LOCUS       DASUGJ010000001        15274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1389392, whole genome shotgun sequence.
ACCESSION   DASUGJ010000001 DASUGJ010000000
VERSION     DASUGJ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15274
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389392"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1346)
                     /gene="coaBC"
                     /locus_tag="VFV93_00005"
     CDS             complement(132..1346)
                     /gene="coaBC"
                     /locus_tag="VFV93_00005"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX5163748.1"
                     /translation="MSILNGKNVVLGVTGGIAAYKAVDLASKLVQAGATVDVIMTRSA
                     TEFLRPLSFEAITKRRVHVDVFEPWSEFEKGHISLADNADLIVVAPATANTIARLAYG
                     MADDMLAVTHLSATPRGVSLVIAPAMEHHMFIHAATQANLATLQARGAWVVGPEHGRL
                     ASGAVGQGRLAAPERIVGVIEQALGASGPLAGKHVVVSAGATREAIDPIRILTNRSTG
                     KMGYAIARSAIQAGARVTLVSAPVHLPPIEGATMVAIESALELRDAVRDHATGADVLI
                     MAAAVADYRPVEVAADKIKKSDDDMAIRLTRNPDILATVETPGTLRIGFAAETRDHEA
                     NARGKLERKQLDLIIANDARLAMASDDNAVTLYYRDGRVETLDRSAKAGIADEIIARV
                     GALLATRRELTS"
     gene            complement(1343..1921)
                     /locus_tag="VFV93_00010"
     CDS             complement(1343..1921)
                     /locus_tag="VFV93_00010"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEX5163749.1"
                     /translation="MIAEPRRAVPLTPRAQAIANRIDAAVLWVVTHWLAVFAGYGTMV
                     VGLALLAPLLESSGHQMAARFIYLPFRLICHQRDDRSFHLAGEKLAFCERDVAIVSAA
                     VLTGLLFAVMRHWRAVPRIGFRAVVACALPMAIDGGTQLFGLRESTAELRVLTGTLFS
                     LGAGWFVLPYLETGFAAIRDEIRGRQAMAGRR"
     gene            complement(1918..2967)
                     /gene="ltaE"
                     /locus_tag="VFV93_00015"
     CDS             complement(1918..2967)
                     /gene="ltaE"
                     /locus_tag="VFV93_00015"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: protein motif:HMM:NF007825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEX5163750.1"
                     /translation="MSLIDLRSDTVTQPTAAMREAMANAVVGDDQYGEDPTVHELEAL
                     AAKMLGKEAAVYVASGTMGNLVSLLAHCGRGDEIIMGDECHIFWYESGGAAALGGMPF
                     NLLPNGPMGELDLERVRKAIRATRPGYPRTGVISIENTQNRCGGTVLPLDYLGELHEL
                     ASSNGVPVHMDGARIFNAAAAIDTPAEEIARHADSVQFCLSKGLAAPVGSVVAGSAEF
                     IQRARAGRKIVGGAMRQSGVIAAAGLVALQTMVDRLPEDHRRARRLAEGLAEIDGISI
                     DLDAVQTNIVIFNVEPNYEQAAFVEAMKRAGIMISNYGTRGVRMVTHYQIDDAAIETA
                     LNAAREVLGAKLAAA"
     gene            3331..6858
                     /locus_tag="VFV93_00020"
     CDS             3331..6858
                     /locus_tag="VFV93_00020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX5163751.1"
                     /translation="MAQRVPTSPSSDDGLPQVVSNTGVNRRSFSSIPTVLEMPNLVQI
                     QINSFEWFKTEGLAELLQEISPITDYNQKMELHFLEHAFDEPRASEEACRERDMTYAA
                     PLRIRVRLVIKETGEIKESDIFLGDFPMMTAHGTFVINGAERVVVSQLVRSPGVYFER
                     EPDPTSGRELVKAKLIPNRGAWLEFETAKTNVLYVKVDRKRKMPVSILLRAIGYEDDE
                     ELHRMFADVDTHPDNHYLQATLDREPTKTREEALVELYKRLRPGDPPTRENASSLLKN
                     LFFDSRRYDLARVGRYKLNKRLELDPTNDTRVLTPDDLVAIIKKMVQLNNMVGEPDDI
                     DHLGNRRIRAVGELIQMHVRTGMQRLERGVKERMTIQDIEQVTPNGLISTTRPVMAAV
                     REFFGGSQLSQFMDQTNPLSEMTHKRRLSALGPGGLSRDRAGFEVRDVHHSHYGRICP
                     IETPEGPNIGLIGSLATYARINQYGFIETPYRRVYSAIRLVTPEGEDVMIRNDDGTES
                     PESDLLIGRELTHDVKAGRKTFAAGTIADADAVAAMKKAGITLARVKPFASDRIEYLS
                     ADREELFTVAQANALLASDGTFLEERVESRRGKGEFPILPPSQVDYMDVSPKQVFSVA
                     TALIPFLEHDDANRALMGANMQKQAVPLLVPNSPIVGTGVEHQAAKDSGQVIVARAPG
                     VVKLVTAREIVIEEDGGEERTYRLQKFVRSNQDTCINQRPSVESGARVEAGQIIADSS
                     STDKGELALGQNVLVAFMPWEGGNFEDAILLSERLVRDDVFTSIHIEKYETEARDTKL
                     GPEEITRDIPNVGEDSLANLDENGIIRIGAEVDPNDILVGKVTPRGETELSAEERLLR
                     AIFGEKAREVKDTSLRVPHGVHGKVIDVKQFRREDNAEHELPAGVNEMVRVMIAQKRK
                     ISEGDKMADRHGNKGVISRILPVEDMPYLPDGTPIDIILNPIGVPSRMNIGQVLETHL
                     GWAAAALGYKIATPVFDGASEEQIEQMLGEAELPLDGQVTLYDGRTGDSFDRPVTVGY
                     IYMLKLAHLVEDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTL
                     QEMLTVKSDDVVGRVKTYEAIVKGDEINEAGVPESFKVLVKELRSLGLSIDVINDDEQ
                     TVDFTEDTSRDLLTNLDRINLSGFERTED"
     gene            6945..11195
                     /gene="rpoC"
                     /locus_tag="VFV93_00025"
                     /pseudo
     CDS             6945..11195
                     /gene="rpoC"
                     /locus_tag="VFV93_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            11749..12174
                     /gene="rpsL"
                     /locus_tag="VFV93_00030"
     CDS             11749..12174
                     /gene="rpsL"
                     /locus_tag="VFV93_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX5163752.1"
                     /translation="MPTINQLVRKGRKPVVKKTKAPALRFTNNAAGRYAGRLRRGKGS
                     PQKRGVCTQVKTMTPKKPNSALRKIARVRLSNGMEVTAYIPGEGHNLQEHSVVLIRGG
                     RVKDLPGVRYHIIRGALDARGVDSRRQGRSKYGAKKPKS"
     gene            12377..12847
                     /gene="rpsG"
                     /locus_tag="VFV93_00035"
     CDS             12377..12847
                     /gene="rpsG"
                     /locus_tag="VFV93_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX5163753.1"
                     /translation="MPRRAKVQRREILPDPKYGSELVTRFVNKVMERGKKGTAERIVY
                     DAFEIVEARMGRNALEVFQGALHNATPIIEVKPRRVGGATYQVPVQIEGGRRLSLAIR
                     WMLASARARNGRTMSEKLAAELMDAFNNTGATIKRREDTHRMAEANKAFAHYRW"
     gene            12955..15066
                     /gene="fusA"
                     /locus_tag="VFV93_00040"
     CDS             12955..15066
                     /gene="fusA"
                     /locus_tag="VFV93_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5163754.1"
                     /translation="MSSTGTMTKGASAGATDLQRTRNIGIIAHIDAGKTTTTERILFY
                     TGRVHRPGETHEGSATMDWMVQERERGITITSAATTCFWLDHRINIIDTPGHVDFTAE
                     VERSLRVLDGGVVVFDAVAGVEPQSETVWRQADKYHVPRIAFVNKMDRTGANYWRTID
                     MMVDRLKAKPAVIQMPIGSEADYLGLIDLFNMVAYIYHDDLGQNIEQTEIPANLMEEA
                     TRRRAELVEAIAESDEDLMLRYLEDEEISVDELMAALRKATISSELVPVLNGSSLKNK
                     GVQRMLDAIIDYLPSPLDVPPVSGIDPKTGEPLTRPTDPDAPFSALAFKVQADPHVGR
                     LTFVRVYSGTMLSGSYAYNSTKGERERVGRLLQMHANHREEIDAVSAGNICAVIGLKS
                     TFTGDSLCDPDHPIILESINFPEPVIEVAIEPKTRQDQDKMGMALARLAEEDPTFRLR
                     TDEETGQTIIMGMGELHLEVIVDRLLREFKVGANVGKPQVAYRETITKPVRIEGRFVR
                     QTGGRGQFGHVWLELEPLEPGAGFVFEDKIVGGVVPREYIPAVGQGVKEAMETGGDAG
                     FPIVDMKVALVDGSFHPVDSSEMAFKIAGSMALKDGVRRASPVVLEPIMKVEVVTPEE
                     FMGDVIGNLNARRGRIEGMEARAGAQVINAFVPLANMFGYTTELRSMTQGRATSTMEF
                     DHYEPLPDALAKELVAKNQRA"
     gene            15138..>15274
                     /locus_tag="VFV93_00045"
     CDS             15138..>15274
                     /locus_tag="VFV93_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5163755.1"
                     /translation="MAKQRFERTKPHINVGTIGHVDHGKTTLTAAITKTLALRGEASF
                     RS"
BASE COUNT         2898 a         4916 c         5005 g         2455 t
ORIGIN      
        1 aggatcgcca gcaggcagcg taggccgaag agcaggacag ccagcgacac gagcgcgatg
       61 atgaccagat cgcgcaccag ccgggtgcgg cgtggcggtc gggtcgcgcg tggtggacga
      121 atcgccatcg atcaggaggt cagttcgcgt cgggtcgcca gcagtgcgcc aaccctggcg
      181 atgatctcgt cggcgatgcc tgccttcgcg ctgcggtcga gtgtctcgac acggccgtcg
      241 cgatagtaga gggtgacggc gttgtcgtcg ctggccatcg ccagccgggc gtcgttagca
      301 ataatcaggt caagctgctt gcgttcgagc ttgccgcgcg cgttcgcctc gtggtcgcgc
      361 gtttcggcgg cgaagccgat ccgcagcgtc cctggtgtct cgaccgtcgc cagaatgtcc
      421 gggttgcgtg ttagccggat cgccatgtcg tcgtcagatt tcttgatctt gtcagccgcc
      481 acctcgaccg gacggtaatc ggcaacggcg gcggccatga tcagtacatc ggcgccggtg
      541 gcgtggtcgc gaaccgcgtc tcgcaactca agcgcggact cgatggcgac catcgtcgcg
      601 ccctcgatcg gcggcaggtg gacgggggcc gaaaccagcg tcacccgcgc gccggcctgg
      661 atcgctgaac gggcgatggc gtagcccatc ttgcccgtcg agcggttggt gaggatgcga
      721 atcgggtcga tcgcttcgcg ggtcgcgccg gcgctgacga cgacgtgctt ccccgccagc
      781 ggtccggatg ctcccagcgc ctgctcgatg acgccaacga tccgctccgg cgccgccagc
      841 cggccctgac cgaccgcgcc gctggccagc cggccatgct ccggcccgac gacccatgcg
      901 ccacgtgcct ggagcgtcgc gaggttggcc tgagtcgcgg cgtggatgaa catgtgatgc
      961 tccatcgccg gcgcgataac gagcgagacg ccgcgcggcg tcgccgagag atgcgtgacg
     1021 gccagcatgt cgtcggccat cccgtaggcc agccgggcga tcgtgttggc cgtcgccggt
     1081 gcgacgacga tcaggtcggc gttgtcggcc agcgagatat gacccttctc gaactcgctc
     1141 cacggctcga agacatcgac gtggacgcgc cgcttggtga tcgcttcaaa tgagagcggg
     1201 cgcagaaact cggtcgccga gcgcgtcatg atgacatcga ccgtcgctcc ggcctggacg
     1261 agcttgctgg ccagatcaac ggccttgtag gcggcgatcc caccggtgac accgaggacg
     1321 acgtttttcc cgttgaggat gctcatcgcc tgccagccat tgcctgccgg ccacggatct
     1381 catcgcggat cgctgcgaag ccggtttcga gataggggag gacgaaccag ccggcaccga
     1441 ggctgaagag cgttccagtc aggacccgca gctccgccgt gctttcgcgc agaccgaaca
     1501 gctgcgtgcc gccgtcgatc gccatcggca gcgcgcaggc gacgaccgcc cggaatccga
     1561 tccgtggcac tgctcgccag tggcgcatga ccgcgaagag caggccagtc aggactgccg
     1621 ccgagacgat tgcgacatcg cgctcgcaga acgccagctt ctcgccggcc agatggaatg
     1681 agcgatcgtc tcgctggtgg cagatcagcc ggaacggcag gtagatgaag cgcgccgcca
     1741 tctgatggcc gctcgattcc agtagcggtg ccagcaacgc cagcccgacc accatcgtcc
     1801 cgtagccggc aaagacggcc agccagtggg tcacgaccca gaggacggcg gcgtcgatcc
     1861 ggttggcgat cgcctgggcg cggggcgtca gcgggacggc gcgacgcggt tcggcgatca
     1921 tgcggcggcg agctttgcgc ccagcacctc gcgggccgcg ttgagcgccg tctcgatcgc
     1981 ggcgtcgtcg atctgatagt gggtcaccat ccggacgcca cgcgtgccgt agttcgagat
     2041 catgataccg gcccgcttca tcgcctcgac gaaggcggcc tgctcgtagt tcggctcgac
     2101 gttgaagatg acgatgttgg tctggacggc gtcgaggtca atcgagatgc cgtcgatctc
     2161 ggccagccct tcggccagcc gccgcgcccg acgatggtcc tccggcagcc ggtccaccat
     2221 cgtctgcagc gcaaccagac cggcggcggc aatcacgcca gactggcgca tcgcgccgcc
     2281 gacgatcttg cggccggccc gcgcccgctg gataaattcg gccgatccgg cgacgaccga
     2341 tccgaccggc gctgccagcc ccttcgagag acagaactgg acgctgtcgg cgtggcgggc
     2401 gatctcttcg gccggcgtgt cgatcgcggc ggcagcgttg aagatgcggg cgccatccat
     2461 gtggaccggg acaccattgc tgctggccag ctcatgcagc tcgcccagat agtcgagcgg
     2521 caggactgtg ccgccgcagc ggttctgggt gttctcgatg ctgatgacgc cggttcgcgg
     2581 gtagcctggc cgagtcgcgc ggatcgcctt ccggacccgt tcaaggtcga gctcgcccat
     2641 cggcccgttc ggcagcaggt tgaacggcat cccgcccagc gccgccgccc cgccggactc
     2701 gtaccagaag atgtggcact catcacccat gatgatctcg tcgccacgcc cgcagtgggc
     2761 cagcagcgag accagattac ccatcgtccc gctggcgacg tagacagccg cttccttgcc
     2821 gagcatcttc gccgccaacg cttcaagctc gtggacggtc ggatcctcgc catactggtc
     2881 gtcgccgacg acggcgttgg ccatcgcctc gcgcatcgcg gccgttggct gggtgacggt
     2941 gtcgcttctc aggtcgatca ggctcacggt ctggctcccg cacattcccg gcggcatctt
     3001 ccgccgcgta acgttttccg gtccggctga ttgtacagat gaagatcgca ggtcgcgaca
     3061 ggttgacaac catcggcctc gggtgtatca tacgaggtcg ctgacgagtg agtgctggcg
     3121 ataacggctg cccttgatat tgatgaaacg cgaaccgata ccagtgtggc ctcgggaggg
     3181 ggaacatctg ccttccggtt ttggcgtttc cgcgtgcagc cgccgacgtc cctcgaacag
     3241 ttcagctgtg cgttctgacc ggtgtctgcc gcatcggtaa cgttcgccgc gcgacccgta
     3301 acacccgtgt ccagtacaaa ggagcaccac atggcccaac gtgttccgac ctcgccgtca
     3361 tccgatgacg gacttcctca ggtcgtctca aataccggcg tcaaccggcg ctccttctcc
     3421 agtatcccga ctgtactgga aatgcccaat ctggtgcaaa tccagatcaa ctccttcgag
     3481 tggttcaaga ccgaaggtct ggccgagctg cttcaggaga tctcgccgat tactgactac
     3541 aaccagaaga tggagctcca cttcctggag catgcgttcg acgagccgcg ggcgtccgag
     3601 gaagcctgcc gcgagcggga catgacctat gccgcgccgc tgcggattcg cgtgcggctg
     3661 gtcatcaagg agacgggtga gatcaaggag agtgacatct tccttggcga cttcccgatg
     3721 atgaccgccc acggcacctt cgttatcaac ggcgccgagc gcgtcgtcgt ctcgcagctg
     3781 gttcgctcgc cgggcgtcta cttcgagcgc gaaccggacc cgacctcagg ccgcgagctg
     3841 gtcaaggcca agctgatccc gaaccggggt gcctggttgg agttcgagac tgccaagacg
     3901 aacgtgctct acgtcaaggt cgaccgcaag cgcaagatgc cggtttcgat cctgctgcgc
     3961 gcgatcggct acgaggacga cgaagagctg caccggatgt tcgctgatgt cgatacccat
     4021 ccggacaacc actacctgca ggcgacgctc gaccgcgagc cgaccaagac ccgcgaagag
     4081 gcgctggtcg agctctacaa gcgcttgcgc cccggcgacc cgcccacacg cgagaacgcg
     4141 tccagcctgc tcaagaacct gttctttgac tcgcgccgct acgatctggc ccgcgtcggc
     4201 cgctacaagc tgaacaagcg actggagctt gacccgacca acgacactcg cgtcctgacg
     4261 ccggacgatc tggtcgccat catcaagaag atggtccagc tcaacaacat ggttggcgag
     4321 ccggacgaca tcgaccacct tggcaaccgg cgcatccgtg ccgtcggcga gctgatccag
     4381 atgcatgtcc ggaccgggat gcagcgcctg gagcgtggcg tcaaggagcg gatgacgatt
     4441 caggacatcg agcaggtcac gccgaacggt ctgatcagca cgacccgacc agtcatggcc
     4501 gccgtccgtg agttcttcgg tggctcgcag ctgtcgcagt tcatggacca gaccaacccg
     4561 ctgtcggaga tgacgcataa gcggcgtctc tcggcgcttg gtcctggtgg tctgagccgc
     4621 gaccgggccg gcttcgaagt ccgcgacgtg caccactcgc actacggccg catctgcccg
     4681 atcgagacgc cggaaggtcc gaacatcggt ctgatcggct cgctggcgac ctacgcgcgg
     4741 atcaatcagt acggcttcat cgagacaccg taccggcgcg tctacagcgc gatccggctt
     4801 gtcacgcccg agggcgaaga cgtcatgatc cgtaacgacg acggcactga gtcgccggag
     4861 tcggacctgc tgatcggccg cgaactgacg cacgacgtca aggctggccg aaagacgttc
     4921 gccgccggaa cgatcgcgga cgccgacgca gtcgcggcaa tgaagaaggc cggcatcacg
     4981 ctggcccgcg tcaagccgtt cgcgtccgac cggatcgagt acctctcggc cgatcgtgag
     5041 gaactcttca cggtcgcgca ggcgaacgcg ttgctggcgt ctgacgggac cttcctcgaa
     5101 gagcgtgtcg agagccggcg cggcaagggc gagttcccga tcctgccgcc gtcgcaggtc
     5161 gactatatgg acgtctcgcc gaagcaggtc ttctcggtgg cgacggcgct gattccgttc
     5221 ctggagcacg acgacgccaa ccgcgcgctg atgggcgcga acatgcagaa gcaggccgtc
     5281 ccgctgctcg tgccgaactc gccgatcgtg ggcaccggcg tcgagcatca ggcagccaag
     5341 gactccggcc aggtcatcgt cgcccgggca ccaggtgtcg tgaagctggt aacggcgcgc
     5401 gagatcgtca tcgaggaaga cggcggcgag gaacgcacct accggttgca gaagttcgtc
     5461 cgttcgaacc aggacacctg catcaaccag cggccgagcg tcgaaagtgg ggcgagggtc
     5521 gaggctgggc agatcatcgc cgactcttcc agcaccgata agggcgagct ggcgctcggc
     5581 cagaacgtgc tggtcgcctt catgccgtgg gagggtggca acttcgagga cgccatcctg
     5641 ctctccgagc ggctggtgcg cgacgatgtc ttcacctcga tccacatcga gaagtacgag
     5701 acggaagcgc gcgacaccaa gctgggtccg gaagagatca ctcgcgacat cccgaacgtt
     5761 ggcgaggaca gcctggccaa ccttgacgag aacggcatca ttcggatcgg cgcggaagtc
     5821 gacccgaacg acatcctggt cggcaaggtc acaccccgtg gtgagacgga gctgtcggcc
     5881 gaggagcgat tgctgcgggc gatcttcggt gagaaggccc gtgaggtgaa ggacacctcg
     5941 ctgcgggtgc cgcacggcgt ccacggcaag gtgatcgacg tcaagcagtt ccgtcgcgag
     6001 gacaacgccg agcacgagct gccggccggt gtcaacgaga tggtccgcgt catgatcgcc
     6061 cagaagcgca agatctccga gggcgacaag atggcagacc gccacggcaa caagggtgtc
     6121 atctcgcgga tcctgccggt cgaggacatg ccctacctgc cggacggcac gccgatcgac
     6181 atcatcctga acccgatcgg tgtcccgtcg cggatgaaca tcggccaggt gctggagacg
     6241 catctcggct gggcggccgc cgcgctcggc tacaagatcg cgacgccggt cttcgacggc
     6301 gcgtccgaag agcagatcga gcagatgctt ggcgaggccg agctgccgct cgacggtcag
     6361 gtcacgctct acgacggccg aaccggcgat tcgttcgacc ggccggtgac ggttggctac
     6421 atctatatgc tcaagctggc gcacctggtc gaggacaaga tccacgcccg ctcgaccgga
     6481 ccgtactcgc tggtgaccca gcagccgctg ggtggcaagg cgcagttcgg cggccagcgc
     6541 ttcggcgaga tggaggtctg ggcgctggaa gcctacggcg cggcccacac cctccaggag
     6601 atgctgacgg tcaagtcgga cgacgttgtc ggccgtgtca agacctacga ggcgatcgtc
     6661 aagggcgacg agatcaacga agccggcgtg ccggagtcgt tcaaggtgct cgtcaaggag
     6721 ctgcggtcgc ttgggctctc gatcgacgtt atcaacgatg acgagcagac ggtcgacttc
     6781 accgaggaca cctcgcgtga cttgctgacc aacctcgatc gcatcaacct gagcggtttc
     6841 gagcgcacag aagactgaac caatcctcgg tgagacggat gggcgcgccc cgacggcgcg
     6901 ggcgcgcctg aagtaaggac cggtgagtaa ccaacatgca gacgatgaga cagacgaacc
     6961 gaggggattc gcggcgttcg ctggacgtga acgatttcga cgcgatccgt atcagtctcg
     7021 cctcgccaca ggatattcgc agctggtcgt acggcgaggt gaccaagccg gagacgatca
     7081 actaccgcac gctcaagccg gagcacggcg gcctgttctg cgaacgcatc ttcggtccga
     7141 cccgcgactg ggagtgctat tgcggcaagt acaagcgcat ccgccacgcc ggcacggtct
     7201 gtgacaagtg cggcgtcgag gtgacgcgct cgaaggtccg ccgcgagcgg atgggccaca
     7261 tcgagctggc cgcgccggtc acgcacatct ggtatgtcaa gggcacgccg agccggctcg
     7321 ggctgctgct cgacgtttcg ccgcgcaacc tggagcgggt gctctacttc gcgtcgtacc
     7381 tcatcaccga ggtcgatatc gaagcgcgcg acgacatggt cgagaagatc aaccaggcgc
     7441 gcgatgaggc gctggccgaa gtcgacgccg agttccagga gcgtgtcgcc gaactgaccg
     7501 gcgcccgcga tgtcgaggtc aagtcactga cgcaaggctc gcagggcgtg accgaggcga
     7561 tcgagaagca gctcgaaacc gacctggccg cgatcgaaga cgaagccaag accgttcgtg
     7621 atctgctgga gaaggctagt ggcgaatcgg ccgaggagga catgctcttc cgcggtcagg
     7681 tcgtcgtgcg cgccggcgag gccgtcaatg acgaccgcgt cgctgccctg aactcgctgc
     7741 gtgacaccgc cgtcgaggag actcgcgcgc gggcagagca cgatatcgag agcgccaacg
     7801 ccctttcggg cgccgaaacc gactcgatca cggccgaggc cagcgagtcg ctgcaccggc
     7861 tggaggctca gtacgacagc cagaagaacg acctgaaggc ggaagccgat cagctcgtca
     7921 agcgcatcaa cggcatcact cagctccagg tcatcaccga gaccgagtac cgagagctga
     7981 tggaggacgc ccccggcgtc ttccgggcca gcatgggcgc cgaggccgtc tacgatgtcg
     8041 tctccaagat cgacatggac gaactggcga caacgctgcg ccaggagatg cagaagatga
     8101 gcggcctgcg gcgcaagaag gcgaccaagc ggctgcgcgt catcgaggcg ctgcgcaaga
     8161 gcggtaaccg gccggagtgg atgatcttca cggccctgcc ggtcatcccg cctgacctgc
     8221 ggccgatggt ccagctcgac ggtggccgct tcgcgacctc cgatctgaac gacctgtatc
     8281 gccgcgtcat caaccgcaac aaccgcctca agcggctgct cgagcttggc gcgccggaca
     8341 tcatcgtccg caatgagaag cgcatgctcc aggaagcggt cgacgcgctg atcgacaacg
     8401 gccggcgcgg ccgggtcgtc tctggctcgg gcaagcacaa gctgaagagc ctctccgaca
     8461 tgctcaaggg gaagcagggg cgcttccgcc agaacctgct aggcaagcgt gtcgactact
     8521 cgggccgttc ggtcatcgtc gtcggcccgg acctgaagct ggacgagtgc gggctgccga
     8581 agcggatggc gctggagctg ttcaagccgt tcgtgatgcg caagctggtc gtccacggcc
     8641 acgcccacaa catcaaggcc gccaagcggc tggtcgagcg cgttgacgag cgggtctggg
     8701 gcgtgctgga agaggtcatt caggactacg tcgtcctcct caaccgcgcc ccaacgctgc
     8761 accggctcgg catccaggct ttcaaggtcc ggctgatcga gggttcggcg atccagattc
     8821 atccgctggt ctgcacggcc ttcaacgccg acttcgacgg cgaccagatg gctgtccacg
     8881 tcccgctctc gcgggctgcc cagcttgagg cgcgcaagcg gatgctctcg accgccaacc
     8941 tgctcgaccc gtccgacggc gacccggtca tcgcgccgac gctggagatc gtgctcggct
     9001 gctactacat gaccaaggag caggagggcg ccaagggcga gggtaaagtc ttcgccagcg
     9061 ctcaggaagc gcagatggcc taccagcttg agctggtgga cctgcacgcg aagatccgcg
     9121 tcttcgaccc gaaggcagcc tcctcgccgc tcgacccgaa ccgcgacggc cgcgtgccgg
     9181 acgggctgat cgagacgacg ctcggccgcg tcctgttcaa tatggagatg ccgtccgagc
     9241 tgcgctactg gaacgagacg atggaccgca agatgctgcg caagctcatc tacgaggcct
     9301 accgtctgtt cggcgccgag gatacggctg agatcgccga ccgcatcaag cagatcggct
     9361 tccgctactc gacgcgtggc ggcctctcga tcggcctcca ggacatcgcg atcccagtcg
     9421 agaagcaggg catcatcgac tcggctgacg agcaggttgc cgtcttcgac cgtcacctgc
     9481 agcgtggcat gatcaccaag caggagcggc acagccaggt cgtcaaggtc tggaacgagg
     9541 cccgcgagga gctgaccaac gccgtctcgc gcgggcttgg cgacgacaac tcgctctata
     9601 tgatgagctt ctcgggcgcc aagggtaatc tgaaccagat cacccagatg gccggcatgc
     9661 gcggtctgat gctggacccg aacgggaaca tcattgacct gccgattcgc tcgaacttcc
     9721 gcgagggtct gtcggtcctc gaatacttca tctcgaccca cggcgcccgc aagggtctgg
     9781 ccgacaccgc cctgcggacg gctgactccg gctacctgac gcgacggctg gtcgacgttt
     9841 cgcaggacgt catcgtcacc gccgacgact gcggcacgac caacggcatc acgatcgacg
     9901 ttgacgaggt gatcgacctc ttcgacgagg agcagttgca ggcacggatc gagggccgga
     9961 tcgcggccga gccgatcgtc actgccgatg gcgagatcct ctgcgaccgc aacgaggaga
    10021 tcaccgaaga ggtctacctc gcgatccggg cggccgacgt caagcagttc cgcatccgca
    10081 cctcgatgga gtgcgagtcg gttcacggcg tctgcgcgat gtgctacggc cgcaaccttg
    10141 ccagtggtga cctggtcggc cacggcgagg cggtcggtat catcgccgcc cagtcgatcg
    10201 gcgagccggg tacacagctg acgatgcgca ccttccacac cggcggcgtc tcgcacgcgg
    10261 acgacatcac gaccggtctg ccccgcgtcg aggagctgtt cgaggcccgc gatccgaaag
    10321 gtaaggcggt cctctccgag atcgaaggaa tcgtcgagat cctcaatgag gaagatgctc
    10381 gcaagatcat catccgcaat cgcgacctga cgaccgagcg ctacgagctt ggcgacgagt
    10441 acgtcccggc tgtcaaggac ggtgacaaga tcgcgcgcgg cgcgacggtt gcgacctcgg
    10501 ccaacggagg tggcatgctc gtctcgtcgc tggacggcct ggctgtcatc gacaccgagt
    10561 cgaacgcgct gctggtccag agcgaggtca tcgatgagaa ggaatacacc gtcccgcaca
    10621 cggcccacgt ccgggttcag gacggggaca gggtcaatcc cggcgaccag ttgatcgacg
    10681 gcaacctcga cccgcaggaa gtgctgacga cacgcggacg agaggccgtc cagcggctgc
    10741 tggtcgatga ggtccagaag gtctacaagt cgcagggtgt tgtcaccaac gacaagcaca
    10801 tcgagacgat cgtccgccag atgctgcgca aggtcagcat cgacgatccg ggcgacacag
    10861 acctgctgcc gggcgaactg gtcgaccgct tcgcgttcaa ccagatgaat gccgagatgc
    10921 tggcccaggg cggcgagccg gcgacggccc aggagatgct gctcggcatc accaaggcat
    10981 cgctggcgac cgattcgttc ctgtcggcgg ccagcttcca ggagacgacc cgtgtcctga
    11041 ccgaagcggc catcaacggc aagatcgact cgctgcgcgg cctcaaggag aacgtcatca
    11101 tcggcaagct gatccccgcc gggaccggct tcgatgtccg gccggacggg accaacgccc
    11161 tgacggctgg cgacgaagcc acgctggctg cactcggcga agagatccgc gaggtcgagc
    11221 cgacctcaat cgaagatgtc gaggagatgc tctcgaccgc caacgccaac gaggcgacca
    11281 acctggcggt tgacaccttc gacgccgacg actcgggcga ggaagtcgct gacgagccgt
    11341 ccgaactcga aggcgccgac gccggcgacc tggccctcac cgacctcttc gccgaagagg
    11401 aagaggacca ggccggcctc gaagccagcg acggcgagga caacgaggtc gacccgacca
    11461 cgaactagga ttcgggtagc agtcgtagaa ggggcgcgga tccgcgcccc ttctgcgtgc
    11521 ccaatcccca ctggaaatgc ctgattgtca tcgaagcgat gggcaaatcg tttgacatag
    11581 aaccgggtcg ttgttacact ttcccattgt ggccggaaaa cgggccgatg tttacgacgc
    11641 tctgcgttct cgatccgtag ctgccgcaac gaccgtgtgg caatcgttga tattgaagca
    11701 ttgccgcgct gtctcaagcg ggctacagcg agggggaaac gggaaaacgt gccgacgatc
    11761 aatcaattgg tccgcaaagg ccggaaaccg gtcgtcaaga agactaaggc gccagccctg
    11821 cgattcacga acaacgcagc cggcaggtac gctggtcggc ttcgccgtgg caaaggttcg
    11881 ccgcagaagc gcggcgtttg cacccaggtg aagacgatga cgccgaagaa gccaaactct
    11941 gcgctccgga agatcgcccg tgtgcggctc tccaatggga tggaagtcac cgcatacatt
    12001 cccggcgagg ggcataacct ccaggagcac tccgtcgtgc tgattcgtgg cggtcgcgtc
    12061 aaggaccttc ctggcgtgcg ctaccacatc atccgcggtg cgctcgatgc gcgtggtgtt
    12121 gatagccgtc gccaggggcg gtcgaagtac ggggccaaaa agccgaagag ctagcgctcg
    12181 ccgtccgagg ggattgcccc tcggataccc gagcggactc tttgtgaaga tcgtacggga
    12241 cgaaaagcgg cctcgcggtg tctgaaacac ctgtcgaccg cttttctgcg tgggtaagag
    12301 aagtcacttc cggggcgata gcgcgatccg gattgcgtac aggacggccg gcggggaccg
    12361 gcgggaggct tgaggaatgc cacgacgcgc caaagtgcaa cgccgcgaga ttctgccgga
    12421 tccgaagtac ggcagtgagc tcgtgactcg cttcgtcaac aaggtgatgg agcgtggcaa
    12481 gaaggggacc gccgagcgga tcgtctacga cgccttcgag atcgtcgaag cgcggatggg
    12541 ccgcaacgcg ctggaggtct tccagggcgc gctccacaac gccacgccaa tcatcgaagt
    12601 gaagccgcgg cgtgttggtg gcgcgaccta ccaggtgccg gtccagatcg agggtggccg
    12661 tcgcctgtcg ctggcgatcc gctggatgct ggcctcggcc cgcgcccgca acggccggac
    12721 gatgtccgag aagctggccg ccgagctgat ggacgccttc aacaacactg gcgcaacgat
    12781 caagcgccgc gaagatacgc accggatggc ggaagcgaac aaggcatttg cgcactatcg
    12841 ctggtaagcc atctgcgata cgccgcaagt gacgacactg tcgccaacga tttgaatcgc
    12901 ctgcgccatg ccggtgcgac gtagcgccgg ccacagggag acgaagtagc aatcatgagt
    12961 agtaccggaa cgatgaccaa gggcgcgtcc gccggcgcga ccgacctcca gaggacgagg
    13021 aatatcggca tcatcgcgca tatcgatgcc ggtaagacga cgacgaccga gcgcatcctc
    13081 ttctataccg gtcgcgtcca ccgtcctggc gagacccacg aaggttcggc gacgatggac
    13141 tggatggtcc aggagcgcga gcgcggcatc acgatcacgt cggccgcgac gacctgcttc
    13201 tggctggatc accgcatcaa catcatcgac acgccaggcc acgtcgactt cacggctgag
    13261 gtcgagcgct cgctgcgcgt tctcgacggt ggtgtcgtcg tcttcgacgc cgttgccggt
    13321 gtcgagccgc agtccgagac cgtctggcgg caggccgaca agtaccacgt tccgcggatt
    13381 gcgttcgtca acaagatgga ccgcaccggc gcgaactact ggcgcacgat cgacatgatg
    13441 gtcgaccgcc tcaaggccaa gccggccgtt atccagatgc cgatcggttc cgaggccgat
    13501 tacctgggcc tgatcgacct gttcaacatg gtcgcctaca tctatcacga tgacctgggc
    13561 cagaacatcg agcagaccga gattccggcc aacctcatgg aagaggcgac ccgccgccgc
    13621 gccgagctgg tcgaggcgat tgccgagtcc gacgaagacc tgatgttgcg ctacctcgaa
    13681 gacgaggaga tctcggtcga cgaactcatg gccgcgctcc gcaaggcgac catctcgtct
    13741 gaactggtcc cggtcctgaa tggctcgtcg ctgaagaaca agggtgtcca gcgcatgctg
    13801 gacgcgatca tcgactatct gccgtcgccg ctggatgtcc cgccggtctc cggcatcgat
    13861 ccgaagactg gggaaccgct gacccgcccg accgatcccg acgcgccgtt ctcggcgctg
    13921 gccttcaagg tccaggccga tccgcacgtc ggccggctga cgttcgtgcg cgtctactcc
    13981 ggcacgatgc tctccggctc gtatgcctac aactcgacca agggcgagcg cgagcgcgtt
    14041 ggccgcctgc tgcagatgca cgcgaatcac cgcgaggaga tcgacgccgt ttcggccggc
    14101 aacatctgcg ccgtgatcgg cctcaagagc acgtttaccg gcgatagcct ttgcgatccc
    14161 gaccatccga tcatcctgga gtcgatcaac ttcccggagc cggtcatcga ggtcgcgatc
    14221 gagcccaaga cccgccagga ccaggacaag atgggcatgg cgctggcccg gctggccgag
    14281 gaggacccga ccttccgcct gcggacggac gaggagaccg gccagaccat catcatgggc
    14341 atgggtgagt tgcacctgga agtcatcgtc gaccggctgc tgcgtgagtt caaggtcggc
    14401 gcgaacgtcg gcaagccgca ggtggcctac cgcgagacga tcaccaagcc ggtccgaatc
    14461 gagggccgct tcgttcgcca gaccggtggt cgcggtcagt tcggccacgt ctggctggag
    14521 ttggagccgc tggagccggg cgccggcttc gtcttcgagg acaagatcgt cggtggcgtc
    14581 gtcccgcgtg agtacatccc ggccgttggc cagggcgtca aggaagcgat ggagaccggt
    14641 ggcgacgccg gcttcccgat cgttgacatg aaggttgccc tcgtcgacgg ctcgttccac
    14701 ccggtcgact cgtccgagat ggcgttcaag atcgccggct cgatggccct caaggacggt
    14761 gtccggcgcg catcgccggt cgtcctcgaa ccgatcatga aggtcgaggt cgtgacgcct
    14821 gaggagttca tgggcgatgt catcggtaac ctgaacgctc gccgtggacg gatcgagggc
    14881 atggaggccc gcgctggcgc gcaggtcatc aacgccttcg tcccgctggc gaacatgttt
    14941 ggctacacga ccgagctgag gtcgatgacg cagggtcggg cgacctcaac gatggagttc
    15001 gaccactacg aaccgctgcc ggacgcgctc gccaaggagc tcgtcgccaa gaaccagcgg
    15061 gcataacgac ggaataatgg tggccggcgg gagtccgccg gcctcggttt gagagttctg
    15121 ggaggcaggg gaacacagtg gcgaagcaaa gattcgagcg gacgaagccg catatcaacg
    15181 tggggacgat cggacacgtc gaccacggca agacgacctt gacggcggcc atcaccaaga
    15241 cgctggcgtt gcggggcgaa gcctccttcc gctc
//
LOCUS       DASUGJ010000002         5478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1389541, whole genome shotgun sequence.
ACCESSION   DASUGJ010000002 DASUGJ010000000
VERSION     DASUGJ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389541"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFV93_00050"
     CDS             <1..345
                     /locus_tag="VFV93_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5163756.1"
                     /translation="MLKDTQADELCRAIKAAAAGQVQLSPEAASRLLREVRAPESPEA
                     LTDRETDVLRLLARGLANKEIAQQLSIGEKTVKTHVSHILSKLGVASRTQAALYAARV
                     GLVPAGEVGERV"
     gene            342..1871
                     /locus_tag="VFV93_00055"
     CDS             342..1871
                     /locus_tag="VFV93_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5163757.1"
                     /translation="MTGSYDTTANAADRLGLDVLASIMDASLDGIVVIDDNRRYLYVN
                     PAACRILGRPASELIGADILINFPAHQHEQVLGVFKQMSLGNGGRNDSTIVLPSGEER
                     QIEFTDTRFMADGRQLRTAIFRDVTESRRQEREARVLAEIAASLTIDQPMEETLDVLT
                     NFVVEETGAKAAVIALIEPETMHLDAFTVRGLPERFMEKMRERWPMSRNSVTVRAIET
                     QQIEVVCNARARMLTNMAYASVHNELVDANWDALAAVPLVYHGESHGALLAYYPNPGP
                     PDGEIALLAAIADQAAIVVENNRLYAEAQGQAAIEERQRLARELHDSVSQALYGIGLG
                     ARTARTLIGQDPARAVAPVEYVLQLAEAGLAEMRALIFELRPESLEAEGLVAAIGKQA
                     ASLFARHRIVVNSVLCDEPAVPLPIKEAVYRIIQEGLHNVVKHSHATTVDLRLECDDT
                     IVRFDIRDNGVGFDPAGEFPGHLGLQSMRERAARLGGVVEIESSPRQGSRIHGFVPCH
                     G"
     gene            2049..2717
                     /locus_tag="VFV93_00060"
     CDS             2049..2717
                     /locus_tag="VFV93_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX5163758.1"
                     /translation="MPEYVGLLGRSFLLGLAIAAPVGPTSMTAIRRGLATGAWAAFWV
                     GMGAALTDFFYIVATYAGFTPLFDRIPGLKTVLYLAGAIVLARIAYGAIKDALTGAGA
                     PVIDAEGVSNIVREPWTALLALGVGVTLINPATITYWVSVGGAFVASSLSGRSVAGAG
                     GMMLAVWAGSAFWFTVLAGIVGVARARIEQLPWLFRAVGCISGFILLAFAIAFAIRGI
                     ERVA"
     gene            2828..3649
                     /locus_tag="VFV93_00065"
     CDS             2828..3649
                     /locus_tag="VFV93_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163759.1"
                     /translation="MRPLSAGDIVDEAIGLYRRNFRLFLSIGAVALVPAGVIQLILAL
                     LADEAGPSLLTISSALWWVLSVVVAGVAYILVFATMAIAASERWLERPMSVSDAYRRM
                     FNRIGALASLVLIYALTIAILTATIIGFPVAIYLAVAWLLSIQILLVEGSGLRNALAR
                     SRRLVAGHWWRVIGIVVLLAIIQGIISAFFTLPATIFGFGSLFSDPTGTDASPLVVAL
                     DAIGSTAGQIVAGPIAYCAYILLYYDLRVRKEGLDIELAARSLGLIDAPAAGSQA"
     gene            3646..4611
                     /locus_tag="VFV93_00070"
     CDS             3646..4611
                     /locus_tag="VFV93_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX5163760.1"
                     /translation="MRLARLAIVVVALALALPLAGSPVTAIDLDTYRAQLEAIDASLS
                     GPNPDIDGALTILSAIEPVTLDDGSNVAPDLSRITTALEADPPDIASAQAGIDAILSA
                     LDLAGTDLASDPDAAVTALDEVLAREEFQPEPVEAESPSLWQRFLGWVNDIITSFFEW
                     LNGLLGGSSGEGSPLSTALALLGVLVIVGIVAFAVRSVRESMTPGVTRLAEGSAEEHY
                     TSAEARDEAERLFAAGDYRAALRMLYLATLIRWEEAGRLRFDRSLTNREVVARVTLHG
                     DATLLAQLSPLVERFDRVWYGGASCTSNDYASFASLAERAWEAAP"
     gene            4608..>5478
                     /locus_tag="VFV93_00075"
     CDS             4608..>5478
                     /locus_tag="VFV93_00075"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX5163761.1"
                     /translation="MTGSRRLPGRGVLVIGGLVVLLVVLAILARSNERKGVSGPPLSM
                     HVTVEDGGRALYIWLADLGYDVRPLEYRADQIDDDADALFILAPLDDLSETESARVLD
                     WVERGGTLLLAAETPNQLLDDLDLSLRSSGVAQTDAVPLQPVFLGPEVQTMRVNATTW
                     LDLPDLSWVPLLGAGDQPVAATAGYGRGRIHVLSSEFLLSNAGIGLAGNAAYPLHVLA
                     GIPRGGTVVFDEYHHGLTEHGTLNQRLVREPWGWAVLWAAAFTFAWLAFSGKRFGKAL
                     RPPPSWARRSSGEY"
BASE COUNT          785 a         1807 c         1886 g         1000 t
ORIGIN      
        1 atgctcaagg acacccaggc cgacgaactc tgccgggcga tcaaggcggc tgccgccggc
       61 caggtccagc tctcgccgga ggccgcctcg cggctgctgc gtgaggttcg cgcgccagag
      121 agcccggagg cgctgaccga ccgcgagacc gacgtgctcc ggctgctggc gcgcgggctg
      181 gccaacaagg agatcgccca gcaactctcg atcggcgaga agacggttaa gacgcacgtc
      241 agccatatcc tctcgaagct cggtgtcgcc tcgcgcaccc aggccgcgct ctacgcagcc
      301 agggtcgggc tcgttcccgc cggggaggtt ggcgaacggg tgtgaccggc tcgtacgaca
      361 ctactgcaaa cgccgctgac cggctcgggc tggatgtcct ggccagcatc atggacgcct
      421 cgctcgacgg catcgtcgtg atcgacgata accggcgcta tctctacgtg aatccggctg
      481 cctgccggat cctcggccgt cccgccagtg agctgatcgg cgcggatatc ctgatcaact
      541 tcccggccca tcagcatgag caggtgctgg gtgtcttcaa gcagatgtca ctcggcaatg
      601 gtgggcggaa cgacagcacg atcgtgttgc cgtccggcga ggagcgccag atcgagttca
      661 ccgacacgcg cttcatggcc gacggccgac aattacggac ggccatcttc cgcgacgtga
      721 ccgaatcgcg ccgccaggag cgcgaggcac gcgtgctggc tgagatcgcc gccagcctga
      781 cgatcgacca gccgatggaa gagacgctcg acgttctgac gaacttcgtc gttgaagaga
      841 ccggtgcgaa ggcggctgtc atcgccctga tcgagccgga gacgatgcac ctcgacgcct
      901 tcaccgtccg tggcctgccg gagcgtttca tggagaagat gcgggaacgc tggccgatgt
      961 cgcgcaactc ggtaacggtt cgtgcgatcg agacacagca gatcgaggtc gtctgcaacg
     1021 cgcgcgcgcg gatgctgacc aacatggcct acgcatccgt ccacaatgag ctggtcgacg
     1081 cgaactggga tgcgctggcc gccgtgccgc tggtctatca cggcgagtcg cacggggcat
     1141 tgcttgccta ctatcccaac cccggcccgc cggacggcga gatcgcgctg ctggctgcca
     1201 tcgccgacca ggccgcgatt gtcgtcgaga acaaccggct ctatgccgag gcgcagggcc
     1261 aggcggcgat cgaggagcgc cagcgactgg cgcgcgagct gcacgactcg gtctcgcagg
     1321 cgctctacgg catcggcctc ggcgcgcgga cggctagaac gctgatcggc caggacccgg
     1381 cccgggccgt cgcgccggtc gagtacgttc tccaactcgc cgaagccggg ctggccgaga
     1441 tgcgggcgct gatcttcgag ttgcgccccg aatcgctcga agcggagggg ctggtggccg
     1501 cgatcggcaa gcaggctgcc tcgctgttcg cccgccaccg catcgtcgtc aactccgtcc
     1561 tctgtgatga gccggccgtc ccactgccga tcaaggaagc cgtctaccgg atcatccagg
     1621 aagggttgca caacgtcgtc aagcactccc acgcgacgac ggtcgatctg cggctggagt
     1681 gcgacgacac gatcgtccgt ttcgacatcc gcgacaacgg cgtcggcttc gacccggccg
     1741 gcgagttccc cggccacctc ggcctccagt cgatgcgcga acgcgccgcc cgcctcggcg
     1801 gcgtcgtcga gatcgagagc agccctcgcc agggctcacg catccacggt ttcgtcccct
     1861 gccacggttg attccatccg gagcagcctg tcatccggta cgggcgaggc actgactcgc
     1921 ccctaccggg tgggctttcg gctctggatt ccatggtgat caacctgagg ccgtatacag
     1981 acgcctgatt caccagtacc atccgcaggc cgtggtggta gtgaaagcgt tgtgggttgg
     2041 acgagttgat gccggagtac gtggggctgc tgggacgttc gttcctgctc gggctggcga
     2101 ttgccgcgcc ggtcgggcca accagcatga cggccatccg gcgcgggctg gcgacgggcg
     2161 cctgggccgc cttctgggtc gggatgggcg cggcgctgac cgacttcttc tacatcgtcg
     2221 ctacctacgc cgggttcacg ccgctgtttg accggattcc cggtctcaag acagtcctct
     2281 accttgccgg agcgatcgtg ctggcgcgga tcgcctacgg cgcgatcaag gatgcgctca
     2341 ccggcgccgg tgcgcccgtc atcgatgccg aaggcgtgtc gaacatcgtg cgcgagccgt
     2401 ggaccgcgtt gctggcgctg ggtgttggcg tgacactcat caacccggcc acgatcacct
     2461 actgggtctc ggtcggtggg gcgttcgtgg cttccagcct gtcgggccgc tcggtcgccg
     2521 gcgccggtgg gatgatgctg gcggtctggg ccgggagtgc cttctggttc accgtcctgg
     2581 ccgggatcgt cggggtcgcc cgtgcgcgga tcgagcagct cccctggctg tttcgcgccg
     2641 tcggttgcat ctccggattc atcctgctcg cctttgcgat cgccttcgcc atccgcggga
     2701 tcgaacgagt ggcgtgagcg gtcgatagac gcggctggac aacgccgggc gtgcgcctgt
     2761 accgttcatg gcaaaccggt gaacgctgac ggctgatcgc tgaagggtag accccgtgga
     2821 gacgaggttg cggccgctgt cggctggcga cattgtggat gaggcgatcg ggctgtatcg
     2881 ccgcaatttc cggctgttcc tgtcgatcgg cgccgtcgcg cttgtgccgg ctggcgtcat
     2941 ccagctcatc ctggcgctgc tggcggacga ggccgggccg agcctcctga cgatctcgtc
     3001 ggcgctctgg tgggtgctgt cggtcgttgt tgcgggagtt gcctacatcc tcgtcttcgc
     3061 gacgatggcg atcgccgcct ccgaacgttg gctcgagcgg ccgatgtcgg tcagcgacgc
     3121 ctaccgccgc atgttcaacc ggatcggcgc gctggctagc ctggtgctga tctacgccct
     3181 gacgatcgcg attctgacgg cgacgatcat tggcttcccg gtcgcgatct atctggcggt
     3241 tgcctggttg ctctcgatcc agatcctgct tgtcgaaggg tcggggctcc gcaacgctct
     3301 cgctcgcagc cgccggctgg ttgccggtca ctggtggcgg gtgatcggga tcgtggtcct
     3361 gctggcgatc attcagggga tcatcagcgc gttctttacg ctgccggcga cgatcttcgg
     3421 cttcggcagc ctcttcagcg acccgaccgg aaccgatgcc tcgccgctgg tcgtcgcgct
     3481 ggatgcgatc ggcagcacgg ccggccagat cgtcgccggc ccgatcgcct actgtgccta
     3541 catcctgctc tactacgacc tgcgcgtgcg caaggagggg ctggacatcg agctggccgc
     3601 ccgctcgctg gggctgatcg acgctcccgc ggctggatcg caggcgtgag gctcgcccgg
     3661 ctggcgatcg tcgtcgtcgc gctggcgctc gcgttgccgc tggccggttc gccggtcacc
     3721 gcgatcgacc tggacaccta tcgcgcccaa ctcgaagcga tcgacgccag cctgagcggt
     3781 cctaatcccg atatcgacgg cgcgctcacc atcctgtcgg cgatcgagcc ggtgacgctc
     3841 gatgatggat cgaatgttgc cccggatctg tcgaggatca cgactgcgtt ggaggcagac
     3901 ccgccggaca tcgccagtgc gcaggcaggg atcgacgcga tactctccgc gcttgatctg
     3961 gcggggaccg atttggcaag cgaccctgac gctgccgtga cggcgctcga cgaggtgctg
     4021 gcgcgggagg agttccagcc agagccggtc gaagcggaat caccgtcgct ctggcagcgc
     4081 ttcctgggct gggtcaacga catcatcacg tcgttcttcg agtggctcaa cgggttgctg
     4141 ggtggcagca gcggcgaggg ctcgccgctt tcgaccgcgc tggcgctgct cggcgtgctc
     4201 gtgatcgttg ggatcgtcgc cttcgccgtt cgcagcgtcc gcgagtcgat gacccctggc
     4261 gtgacccggc tggcggaggg cagtgccgag gagcactaca cctccgccga agcgcgggac
     4321 gaagccgagc ggctctttgc agccggcgac taccgggcgg ccctgcggat gctctacctg
     4381 gcgacattga ttcgctggga ggaggccggc cggctgcgct ttgatcgctc gttgaccaat
     4441 cgcgaggtcg tcgcccgtgt cacgctgcac ggtgacgcga cgttgctggc gcagctctcg
     4501 ccgctggtcg agcgcttcga tcgggtctgg tacggcggcg cttcctgcac gtcgaatgac
     4561 tacgccagct tcgcgtcgct cgccgagcgc gcctgggagg cggcgccatg accggctcgc
     4621 ggcggctgcc gggcaggggt gtgctggtga tcggcgggct ggtcgtcctg ctggtcgtcc
     4681 tggcgatcct cgcccgttcg aacgagcgca aaggcgtcag cgggccgccg ctctcgatgc
     4741 acgtgacggt tgaagacggt ggccgtgcgc tctatatctg gctggccgat cttggctacg
     4801 atgtccggcc gctggagtat cgcgcggatc agatcgatga cgacgccgac gcgcttttca
     4861 tcctcgcgcc gctggacgac ctgagcgaga cggagtcggc gcgcgtcctt gactgggtcg
     4921 aacggggcgg cacgctgctg ctggcggctg agacgccgaa tcagcttctc gatgacctgg
     4981 atctctcgct gcgttcgtcc ggcgtcgctc agaccgacgc cgttccgctt cagccggtct
     5041 tcctcggtcc tgaggtgcaa accatgcggg taaacgcaac gacctggctg gacttgccgg
     5101 atttgtcgtg ggtgccgttg ctcggtgccg gggatcagcc ggtggcggcg acggccggct
     5161 acggtcgcgg ccggatccat gtactgtcga gcgagttcct gctctcgaat gccgggatcg
     5221 gcctcgccgg caacgccgcc tacccgctgc acgtgctggc tggcatcccg cgtggcggca
     5281 cggtcgtctt cgacgagtat caccacggct tgaccgagca tggcacgctc aaccagcgac
     5341 tggtgcggga gccgtggggc tgggccgtcc tctgggcggc ggcgttcacc tttgcctggc
     5401 tggcgttctc cggcaagcgc ttcggcaagg cgttgcgacc gccgccaagc tgggcgcgcc
     5461 ggtcgagcgg cgagtacg
//
LOCUS       DASUGJ010000003         9022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1250627, whole genome shotgun sequence.
ACCESSION   DASUGJ010000003 DASUGJ010000000
VERSION     DASUGJ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250627"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFV93_00080"
     CDS             <1..447
                     /locus_tag="VFV93_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5163762.1"
                     /translation="STGQRADRSDIERAARDAQADGFVRALPEGYETVIGERGVTLSG
                     GQRQRLAIARALLTDPHILILDDSTSAIDSATEDEIQQAIRRLQEGRTTLLITHRLSQ
                     IRWADTVLVLRQGHVIDQGTHDELLARCDLYRRIFAHYDVAVTADA"
     gene            491..2314
                     /locus_tag="VFV93_00085"
     CDS             491..2314
                     /locus_tag="VFV93_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5163763.1"
                     /translation="MGFIMDGLDAEAYDRSYGDKVLVRRVIGYFRPKLPIMLFVASMV
                     VVASVSDAGLNLLLAKGIDQLEGSIEGSTVGLIVAALLVAGGVSWTTNFLRQRYTARA
                     VGDVVLTLRKDAFRAVMARDMSFYDEYPSGKIVSRVTSDTEEFASVVTLTLSLLSQLL
                     LFLLVTGILVYVNFTLSLFVLAMAPFIVAIALLFRRYARLATQRAQRSTARVNANVQE
                     AVSGISVAKAFRQEAAVYDEFQSINRQAYQATLRSGFIYNGIFPILVTVAACGTAVVV
                     YFGGQRVLDGDVSAGDWFLFVQSINLFWFPLTSIASFWSQFQLGLAAAERVFALIDAE
                     PRVVQTGDQPAPRLQGRIEFRDLDFSYSPRQRVLDGFNLTIEAGETVAFVGHTGAGKS
                     TLGRLVSRFYEFEDGQLLIDGHDIRSFNLASYRRQLGVVPQAPFLFSGTIADNIRYAR
                     PDATDAEVETVARQVGSGDWLEALPNGLATEVGEAGRGLSMGQRQLVALARVLLQDPA
                     IVIMDEATASVDPLTEAQIQEGLDTVLAGRTAIVIAHRLSTIREADRIIVLREGRIVE
                     EGSHEMLMLRGGHYADLYNTYFRHQSPDYRPGEGFVPVRVA"
     gene            complement(2596..2850)
                     /locus_tag="VFV93_00090"
     CDS             complement(2596..2850)
                     /locus_tag="VFV93_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX5163764.1"
                     /translation="MSDVTVVQLREGLSDLLGRAQHGGERVRITRNGKPVAAIVSVED
                     LELLRALEDRIDLDAARAALQEAHDTGYLAWETVRSEDGR"
     gene            3118..4032
                     /locus_tag="VFV93_00095"
     CDS             3118..4032
                     /locus_tag="VFV93_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX5163765.1"
                     /translation="MLDLSSRTDGFVWLGDHNAPLQRIGGKASQLSRLAGLHRVPPGF
                     CISADLFGASHQPRLTSELREEIAASYRLLAERAGIDEPRVAVRSSAIDEDGPLASFA
                     GQHETFLNVVGIEAVIEAVERCLASATTVQALAYRQQHGLATEGIGIAVLVQQLVLAD
                     VSAVLFSANPISGNRQEMVLTASWGLGESVVGGTVSPDTWMIDAASHDIIVSRIGSKQ
                     RMTVAIPGGTREVEVPRVLREQPSLTDAQIREIAQLGARLEAALGWPVDIECAFAGGE
                     LYVLQCRPITTLGQLPASERGDALEPVA"
     gene            4126..5808
                     /locus_tag="VFV93_00100"
     CDS             4126..5808
                     /locus_tag="VFV93_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX5163766.1"
                     /translation="MSMTTPTAVPAPMTPAWEQPEDAKLFWRQDRMHFPYPITRMEDD
                     LAQYGLAAGMNLAHELYDIPNRLQARRFWTYNYGSMAPLPLTPEEMQAMAARSEQKMR
                     TIIGRLDEEWRSVWLPAIQQHLEHFRDFDLSGASDAALMEHFENAIERIRALWQLHFE
                     IVVPAYLAMNLFDELYRELFSDASAFRPYQLLQGLPNKTVESGNALWRLSRMAAGSTV
                     IRDIFVLYPPRDVVPALESIPEGQDFLIQFHAFLQEFGKRGESWGLSYPTWIEDPTPV
                     VKTIRDYLNNPAHNPIRDQRELAEEREQLIADSRSVLASYPEAVRGQFEGLLAAAQAG
                     VVLSEDHGYWIDFQGMYEVRRLFMEIGRRFAAAKVLTAAENIFHLSFDEIRDTARYLP
                     RVDRRALVAVRRAEMEHYRSITPPPAVGTPPPDMQEGNDNPGARTASKFFGGPPPVSD
                     DPDVLFGAAGSAGVVRGRARVIHDIADAARLQPGEIMVTATTAPPWTPLFGTAVAVVT
                     DTGGVLSHCAVVAREYRIPAVVGTGFATTAITDGQIIEVDGERGVVRIIRGA"
     gene            5810..7243
                     /locus_tag="VFV93_00105"
     CDS             5810..7243
                     /locus_tag="VFV93_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5163767.1"
                     /translation="MTADRQAMGQQAGRVVLLTATLGTMLAPLNSTMIVVALPEILDD
                     FGRSLAWGSWIVISYLVAMAAVQPLGGSLGDRYGRRRMLLVGFSVFLVATVAAALAPS
                     VEVLLVARTVQALAGAMAIPNGTALVRALVPRERQGRTFGTIGSAIAVAAAAGPPLGG
                     VITDAVGWRWIFVANVLLLVPAIALARRLPADAGNPDGKFDLRGAFLMTGGLVTLALA
                     LTSWRLSGVPVWSAGVFGLLALAAGVGLRRHVRRTPKPILNLGLFQRPGFLPASLTVL
                     FSNLTMYTTLLALPVFLTERQDWRSSQVGLLLAGMSVQMIIFSPLGGRLSDQRGRRFP
                     AVVGTVLTVIGSLPLLALSDSWDWWVLLPPLVVVGVGLGLSTAAVQTAAIEVVSTSES
                     GQAAGLFSTMRYLGSILGSAMLAAILVGETPSLDAFRLLFGVLVVTALAAVVTASRLP
                     GPETQLSTDTLQPLDIAPVAPSVATNQ"
     gene            complement(7245..8096)
                     /locus_tag="VFV93_00110"
     CDS             complement(7245..8096)
                     /locus_tag="VFV93_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5163768.1"
                     /translation="MSDHEWYEVKAIGDGITIVGEPLHEENIKSYLVEGERDVAVIDT
                     GLGVGDFAGLVGRLSAKAPVVLQTHGHWDHIGGSHAFERVLVHESEAYALRRGFSNAM
                     YAPLFSPQRVKKAQLPDGFDRATAAIPPAEPTGTLDTGDTVDLGGRMLEVFHTPGHSQ
                     GGLSFLDRQTGYLFAGDAVNYGGMWLFLPRSDPAAFRETARLLAGLADRVSAVYPAHG
                     KVPLPPDDLRRVHEQYEEIWAGRTPDRHVKWDIGFPERVPVDVHDFGEFTYLMGAGRY
                     GESANER"
     gene            8283..>9022
                     /locus_tag="VFV93_00115"
     CDS             8283..>9022
                     /locus_tag="VFV93_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5163769.1"
                     /translation="MTAGQLTDERINELKQRALAKVDEIASQLIEISDWMYENPELGL
                     QERGAVERLTAMLERHGASVDRQIAGLETAFQATFPAPAGEPKVAILAEYDALPEVGH
                     GCGHNIIATAAIGAGIAVGAIAPELPGAAIVLGCPAEESAVPDAGGKIPLIEHGYFDD
                     VDAAIMIHPGTEDYVPLDTSLVAYGISFEFHGKPAHAAAAPHEGINALDAIIQTFNGI
                     SALRQQLKPDVRIHGVITHGGKAPNVIPP"
BASE COUNT         1422 a         3156 c         2882 g         1562 t
ORIGIN      
        1 agcaccggcc agcgggccga ccgctccgat atcgagcggg ccgcgcgtga cgcccaggcg
       61 gatggcttcg tgcgcgcgct gccagaaggc tacgagacgg tgattggcga gcgcggcgtg
      121 acgctctccg gcggccagcg ccagcggctg gccatcgctc gcgcgctgct gaccgatccc
      181 cacatcctga ttctcgacga ctcgaccagc gcgatcgact cggccaccga ggacgagatc
      241 cagcaggcga tccgtcgctt gcaggagggc aggacgacgc tgctcatcac ccatcggctc
      301 tcgcagatcc gctgggccga caccgttctc gtcctccgcc agggccacgt catcgaccag
      361 ggcacccacg acgaactcct ggcccgctgc gacctctacc gccgcatctt tgcccactac
      421 gacgtggcgg tgacggctga tgcgtaacgc gcctacacga cgtcgtgatc cccggcaccg
      481 gaggtaatca ttgggcttca tcatggacgg cctcgacgcc gaggcgtacg acaggtcata
      541 tggcgacaag gtgctggtgc ggcgcgtcat cggctacttc cggccgaagc tgccgatcat
      601 gctcttcgtc gccagcatgg tcgtcgtcgc gtcggtctcg gatgccgggc tgaacctgct
      661 gctggccaaa ggcatcgacc agcttgaggg cagtatcgag ggcagcacgg tcgggttgat
      721 cgtggccgcg ctgctggtcg ctggcggcgt ctcgtggacg accaatttcc tgcgccagcg
      781 ctacacggcg cgggccgtcg gcgatgtcgt cttgacgctg cgcaaggatg ccttccgggc
      841 cgtcatggcg cgtgacatgt cgttctacga cgagtacccc tccggcaaga tcgtctcgcg
      901 ggtgacctcc gacaccgagg agttcgcctc ggtcgtgacg ttgacgctct cgctgctctc
      961 gcaactgctg ctgttcctgc tggtgaccgg catcctcgtc tacgtcaact tcaccctctc
     1021 gctctttgtc ctggcgatgg cgccgtttat cgtcgcgatc gcgctcctgt tccggcgcta
     1081 cgcgcggctc gcgacccagc gcgcgcagcg ctcgacggcg cgagtgaacg ccaacgttca
     1141 ggaggccgtc tccggtatct ccgtcgccaa ggcgttccgc caggaagcgg ccgtctacga
     1201 cgagtttcag tccatcaatc ggcaggccta tcaggcgacg ttgcgctccg gtttcatcta
     1261 caacggtatc ttcccgatcc tcgtgacggt cgccgcctgc gggacggccg tcgtcgtcta
     1321 cttcggcggc caacgggtgc tggatggcga cgtttcggcc ggcgactggt tcctgttcgt
     1381 ccagtcgatc aacctgttct ggtttccgct gacgagcatc gcgtccttct ggagccagtt
     1441 ccagcttggc ctggcggccg cagagcgcgt cttcgcgctg atcgacgccg agccgcgcgt
     1501 cgtccagacc ggtgaccagc cagccccgcg tctgcaaggc cggatcgagt tccgcgatct
     1561 cgacttctcc tactcgccgc gccagcgcgt gctggacggc tttaacctga cgatcgaggc
     1621 cggtgagacg gtcgccttcg tcggccacac cggcgccggc aagtcgacgc tgggccggct
     1681 ggttagtcgc ttctacgagt tcgaggatgg ccaactgctg atcgacggcc atgacatccg
     1741 ctcgttcaac ctcgcctcat accggcgcca gctgggcgtc gtcccacagg cgccgttcct
     1801 cttctccggc acgatcgccg acaacatccg ctacgcccgc cccgacgcga ccgacgccga
     1861 ggtcgagact gtcgcccgcc aggtcggcag cggcgactgg ctcgaagcgc tgccgaacgg
     1921 gctggcaacc gaggtcggtg aggcgggccg tggcctctcg atggggcagc gccagctcgt
     1981 cgccctcgcg cgggtgctgc tccaggaccc ggcaatcgtc atcatggacg aggcgaccgc
     2041 cagcgtcgat ccgttgaccg aggcccagat ccaggaggga ctcgacaccg tcctcgccgg
     2101 ccggacggcg atcgtcatcg cccatcgcct ctcgaccatc cgcgaggccg accggatcat
     2161 cgtcctccgc gaaggccgga tcgtcgaaga gggcagccac gagatgctga tgctgcgcgg
     2221 cggccactac gccgacctct acaacaccta cttccgccac caatcccccg actaccgccc
     2281 cggcgaaggc ttcgtcccgg tccgcgttgc gtagcgccgc tagaggtccc tcagcgcgtc
     2341 gtagacgtcc cgacgattcc cgatcagcac aacgatgacg gcgaggacat cgtcctgcac
     2401 ttcatagatg atccgctaat cgccgacacg gacgcgaaat agcgcatcgg cgccggcgat
     2461 cttttcgcaa ccgctgggac gcggatcagc accaagccgg tcgatgcgat ttcggatgcg
     2521 ctccttctgg ttcgggggaa gcgttctgaa ctgccgtgca gccgccggcg ttagttcgat
     2581 tcggtaggcc atcgcctagc gaccgtcctc ggatcggacg gtctcccagg caaggtagcc
     2641 ggtgtcgtgc gcctcttgca gcgcggcccg cgccgcgtcc agatcaatgc gatcctcgag
     2701 cgcgcgcaga agctcgaggt cttcaaccga gacgattgcc gcgacaggct tgccgttgcg
     2761 cgtgatccgc acccgttcgc caccgtgctg cgctcggccg agcaagtcac tgagtccctc
     2821 acgaagctga acgacagtaa cgtcggacat gggaacgcct ccattctgta cagaatgtac
     2881 attctgtaca tcaagtcgtc aacggagatg tgtttgtcgc gggagtgcgg cgggatacct
     2941 cgccgctgca acctgtccgg atggacaggc ccggcaatgt cctattgcac cagtgatttc
     3001 tcatccgttg gacgtacgag tgatccagca acctgcttac catcgaattc acatttgtga
     3061 aggccggtgg gcgctcgccg accgtttgcg cgagtgcgat cgctggaggt tgctgacatg
     3121 ctcgatctct cgtccaggac tgacggcttc gtctggcttg gtgaccacaa tgcgccgttg
     3181 cagcgcatcg gcggcaaggc gtcccagttg agccggctgg ccggcctgca tcgcgtcccg
     3241 cccgggttct gcatcagcgc cgacctgttc ggcgccagcc accagccgcg gctgaccagc
     3301 gaattgcgcg aggagatcgc ggcttcctac cgtctgctcg ccgagcgggc agggatcgac
     3361 gagccgcgtg ttgccgttcg ctcgtcggcg atcgacgagg acgggccgct ggcgtcgttc
     3421 gccggtcagc acgagacctt cctgaacgtg gtcggcatcg aggccgtcat cgaggcggtc
     3481 gagcgctgcc tggcgtcggc gacgaccgtc caggcgctcg cctaccgcca gcagcacggt
     3541 ctcgcgacag aggggatcgg gatcgccgtc ctcgtccagc agttggtcct cgccgatgtc
     3601 tcggctgtcc tcttcagcgc gaacccaatc agcggtaacc ggcaggagat ggtcctgacg
     3661 gccagctggg gcctcggcga gagcgtcgtc ggcgggaccg tctcgcccga cacttggatg
     3721 atcgacgccg ccagccacga catcatcgtc agccggatcg gcagcaagca gcggatgacg
     3781 gtcgccatcc ccggcggcac ccgcgaggtc gaggtgccga gggtcctgcg cgagcagcca
     3841 tcgttgacgg acgcccagat tcgcgagatc gcgcaactcg gcgcccgtct tgaagcggcg
     3901 ctcggctggc cggtcgatat cgagtgcgcc ttcgcgggcg gagaactcta cgtgctgcag
     3961 tgccgcccga tcacgacgct cggccagctg ccggcgtcgg agagaggaga tgccctcgaa
     4021 ccggtcgcct gaacgacact gaatgtccac tgagcattga tcccgccggc atcgacgctg
     4081 gcggccgaga gcgacgacgc tctacaggat gaaggacatg tcccaatgtc gatgaccacc
     4141 ccaaccgctg ttcccgcgcc gatgacgccc gcttgggagc agccagagga tgcgaagctg
     4201 ttctggaggc aggatcggat gcacttcccg tatccgatca cccgcatgga ggacgatctg
     4261 gcccagtacg gcctggccgc tgggatgaac ctggcgcacg agctgtacga catcccgaat
     4321 cgactccagg cgcgtcgatt ctggacctac aactatgggt caatggcccc gctgccgctc
     4381 acgcccgagg agatgcaggc gatggcagca cgctcggagc agaagatgcg gaccatcatc
     4441 ggccggctcg acgaagagtg gcggtcggtc tggctgccgg ctatccagca gcacctggag
     4501 cacttccgcg acttcgacct gtccggcgca tccgatgccg cgctgatgga gcacttcgag
     4561 aacgcgatcg agcggattcg agcgctctgg cagctgcact tcgagattgt cgtgccggcc
     4621 tacctggcga tgaatctgtt cgacgagctc tatcgcgagc tgttcagcga tgcttcggca
     4681 ttccgcccgt accagctcct gcaagggcta cccaacaaga cggtcgagtc gggcaacgcg
     4741 ctctggcgac tgagccggat ggcggccgga tcgacggtca tccgcgacat cttcgtactc
     4801 tacccgccgc gcgacgttgt cccggcgctc gagtccatcc cggaggggca agacttcctg
     4861 atccagttcc acgcgtttct gcaggaattc ggcaagcgcg gcgagagctg gggattgtcc
     4921 tatccgacct ggatcgaaga tccgacgccg gtcgtcaaga cgatccgcga ctacctcaac
     4981 aaccccgccc acaatcccat ccgcgatcag cgcgaactgg ccgaggagcg cgagcaactg
     5041 attgccgact cgcggtcagt actcgcgagc tacccggaag cggtgcgagg tcagttcgaa
     5101 ggtctgctgg ctgccgccca ggccggcgtc gtcctctccg aggatcacgg ttactggatt
     5161 gacttccagg ggatgtacga ggtgcgacgc ttgttcatgg agatcgggcg acgcttcgca
     5221 gccgcgaagg tactgacggc agccgaaaac atcttccatc tctcgttcga cgagatccgc
     5281 gatacggccc gctacctgcc gcgagtcgat cgccgcgcgc tggttgctgt ccgtcgggcc
     5341 gagatggagc actaccggtc gatcacgcca cccccggccg tcggcacccc accacccgac
     5401 atgcaggaag gcaacgacaa tcctggtgcg cgaacggcca gcaagttctt cggcgggccg
     5461 ccaccagtct ccgacgatcc ggatgtcctc ttcggtgcgg ccggctcggc tggcgtcgtt
     5521 cgtgggcggg cgcgggtgat ccacgatatc gccgacgcgg cccggctcca gcccggcgag
     5581 atcatggtca ctgcgacgac cgccccgccg tggacgccgc tcttcgggac ggcagtcgct
     5641 gtcgtgaccg acaccggtgg tgtcctgagc cactgcgccg tcgtcgcccg cgagtaccgc
     5701 atcccggctg tcgtcgggac cggcttcgcg acgacggcga tcactgacgg ccagatcatc
     5761 gaggtcgacg gcgagcgtgg cgtcgtccgg atcatccggg gcgcctgaga tgacggctga
     5821 ccggcaggcg atgggccaac aggccgggcg ggtggtcctg ttgaccgcga cgctcggcac
     5881 gatgctggcg ccgctcaact cgacgatgat cgtcgtcgcc ctgccggaga tcctcgacga
     5941 cttcggccgc tcgctggcct ggggctcatg gatcgtcatc tcctacctgg tcgcaatggc
     6001 tgccgtccaa ccgctgggcg gcagcctcgg cgaccgctac gggcggcgac ggatgctgct
     6061 ggtgggcttc agcgtcttcc tggtcgcgac cgtcgccgcc gctctcgcgc cgagtgtcga
     6121 ggttcttctg gtcgcccgga ccgttcaggc gctggccgga gcgatggcga tcccgaacgg
     6181 aacggccctc gtccgcgcgc tggtcccgcg tgaacggcag ggacgcacgt ttgggacgat
     6241 cggctcggcc atcgccgtcg cggctgccgc cggcccgccg ctgggcggcg tcatcaccga
     6301 cgccgtcggc tggcgctgga tcttcgtcgc caatgtcctg ctgctggtcc ccgcaatcgc
     6361 tctcgcccgc cgtctgccgg ccgacgccgg caaccctgac ggcaagttcg acctgcgcgg
     6421 tgccttcctg atgaccggcg ggctggtgac actggcgctg gcgctgacct cgtggcgact
     6481 ctccggtgtg ccggtctgga gcgcgggtgt cttcgggctg ctggcgcttg cagccggcgt
     6541 gggcctgcgc cggcacgtcc ggcgaacgcc gaagccgatc ctcaatctcg gtctcttcca
     6601 gcgccccggc ttcctgccgg cctcgctgac ggtgctgttc agcaacctga cgatgtacac
     6661 gacgctgctg gcgctgccgg tgttcctgac cgagcgtcag gactggcgca gcagccaggt
     6721 cggcctgctg ctggccggga tgtcggtcca gatgatcatc ttctcaccgc tcggcgggcg
     6781 gctttccgac cagcgtggcc ggcgcttccc ggccgtcgtt gggacagtgc tgaccgtcat
     6841 cggtagcctg ccactgctcg ctctcagcga cagctgggac tggtgggtgc tgctgccgcc
     6901 actggtcgtc gtcggtgtcg gcctcggcct ctcgacggcc gccgtccaga cggccgccat
     6961 cgaagtcgtc tcgaccagcg aatccggcca ggcggccggc ctcttctcga cgatgcgcta
     7021 cctcggcagc atcctcggct cggcgatgct ggcggccatc ctcgtcggcg aaacgccgtc
     7081 gctcgacgcg ttccgcctcc tcttcggcgt gctggtcgtg accgcgctcg ccgccgtcgt
     7141 gaccgcctct cgcctgccgg gtcccgagac acagttgtca actgacacgc ttcagccgct
     7201 cgacatcgcg ccggttgcac ccagcgtcgc gacgaaccag tgattcagcg ctcgttggcc
     7261 gattctccgt agcgccctgc acccatcagg taggtgaact cgccgaagtc gtgaacatcg
     7321 accggcacac gctccgggaa accgatgtcc cacttcacat gccggtccgg cgtccgcccg
     7381 gcccagatct cctcgtactg ctcgtggacg cggcgcaggt cgtcgggcgg cagcggcacc
     7441 ttgccatggg ccgggtagac ggcgctgacg cggtcggcca gaccggccag cagccgggcc
     7501 gtctcacgaa acgcggccgg gtccgagcgg ggcaggaaca gccacatccc gccgtagttg
     7561 acggcatccc cggcgaacaa atatcccgtc tgccggtcga ggaacgacag cccgccctgc
     7621 gagtggccgg gagtgtggaa cacctccagc atccggccgc ccagatcgac cgtgtcgccg
     7681 gtgtcgagcg tcccggtcgg ttcggccggc gggatcgccg ccgtcgcccg gtcgaacccg
     7741 tccggcaact gcgctttctt cactcgctgc ggcgaaaaca gcggcgcgta catcgcgttc
     7801 gagaacccgc gccgcagcgc gtacgcctcg ctctcgtgga ccagcacccg ctcaaaggca
     7861 tggctgccgc cgatgtggtc ccaatgccca tgcgtctgca acacaaccgg cgccttcgcg
     7921 gacaagcgcc ccaccagccc ggcgaagtcg ccgacgccca gcccggtgtc gatcacggcg
     7981 acgtcccgct caccctcgac cagatacgac ttgatgttct cctcatgcag cggctcgccg
     8041 acgatcgtga taccatcgcc gattgccttc acctcatacc actcgtgatc ggacacccaa
     8101 cctccttcga cgtacgcctg gacgcgtctg gaatgcacct gtccccaatc ttcgaccacg
     8161 attgtaagcc gcaatcgatc ctgttattcc tattccctaa tcccgcgctt tcggctatgg
     8221 tcgccttccg gcgcggcatg cgaggtcgag tcgccgtgtc gagtggagga tcggagagag
     8281 tgatgacagc cggccagctg actgacgagc gcatcaacga gttgaagcaa cgtgcgctgg
     8341 cgaaggtcga cgagatcgcc agccagttga tcgagatcag cgactggatg tacgagaatc
     8401 cggagctagg cctccaggag cggggcgccg ttgagcggct gacggccatg ctggagcgcc
     8461 acggcgcctc ggtcgaccgg cagatcgccg ggctagagac ggccttccag gcaacgttcc
     8521 cggcgccggc cggcgagccg aaggtcgcca tcctggccga atatgacgcg ctgccggaag
     8581 tcggccacgg ctgcggccac aacatcatcg cgacggccgc gatcggcgct ggaatcgccg
     8641 ttggcgcgat cgcgcctgaa ctgccgggcg ctgccatcgt cctcggctgc ccggccgagg
     8701 aatcggccgt cccggacgcc ggcggcaaga tcccgctgat cgagcacggc tacttcgacg
     8761 acgtcgatgc cgccatcatg atccaccccg gaacggagga ctacgtcccg ctcgacacct
     8821 cgctggtcgc ctacggcatc agcttcgagt tccacggcaa gccggcccat gccgccgccg
     8881 cgccgcacga gggcatcaac gcgctcgacg ccatcatcca gaccttcaac ggcatctccg
     8941 ccctgcgcca gcagctcaag ccggacgttc gtatccacgg cgtgatcacc cacggcggca
     9001 aggcgccgaa cgtcatccct cc
//
LOCUS       DASUGJ010000004         5472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_637, whole genome shotgun sequence.
ACCESSION   DASUGJ010000004 DASUGJ010000000
VERSION     DASUGJ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..775)
                     /locus_tag="VFV93_00120"
     CDS             complement(164..775)
                     /locus_tag="VFV93_00120"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5163770.1"
                     /translation="MNIELGADIYGNDGEKLGVVDSLVVEPGNGEIESIIVRKGLFFP
                     TDKILPATAVTSIDDKGVHINLSKHDVEQLTEYMDAEYVWPPAGYYGNYGYIWPATSV
                     YATDLLIDEEVKERNPNAVILSEGTLVVDSNNEEVGRITEMATDERGRVSGFRVEQGF
                     FRHHEHYIPAHLVKTADGAVVHLSISKDRLESITGPEAERRYA"
     gene            993..1685
                     /locus_tag="VFV93_00125"
     CDS             993..1685
                     /locus_tag="VFV93_00125"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5163771.1"
                     /translation="MPAAPMPLDPRPALVLFDLDRTIADFDGARLARTQYAFEPHFVE
                     PDLLDRAVADAVAHASEGSDHFEQVLAGYGIREPEQIELARQRYIEDRYRGLRLFDDA
                     LEVIAAVSLVAAVGLVTNGPSAIQRPKIELLAIEEHFPLIIVSEEVGVWKPDPGIFAI
                     ALERAGVAAANAIYVGDSAEHDIPGAHAAGLRAIWVNRLGIDWPGALPPDAEIRNLYQ
                     LLPLLGIDREDR"
     gene            1721..2914
                     /locus_tag="VFV93_00130"
     CDS             1721..2914
                     /locus_tag="VFV93_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5163772.1"
                     /translation="MSRGTVVIWAAAAIFLLGAIFFAARDDGDANETGTTPIVSGAGG
                     VASTTTATSSGEQTTGNPNATESATGNATPDSDGSIPPTQQPVTESPSFPPNELGQIP
                     ILEYHHLGPEPEQFVRTPDQFRGDLQWLYEHGFHVVSLHDILTDNINIPAGKRPVALT
                     FDDSPASQFSLIPLSDGRLAIDPNCVVGILEHFFIQHPDFGRGGHFAILPQMLFDWAP
                     VEDRSDQAPYAKEKLEWLVANGYEIGNHTYDHVDLSTLTNDQIMYQLAEDNIAIHELV
                     PDVPVEVVTLPYGMYPAGGDDTLFRGFDYKGQHYAFTAALLVGANPTVSPLSTEYDPY
                     STARIQAFDEELDKWFGVFTDQPGILYVSDGDPNTVTVPKDLHPWLVDTLDMSKIGNR
                     ELVRY"
     gene            3041..3997
                     /gene="ispH"
                     /locus_tag="VFV93_00135"
     CDS             3041..3997
                     /gene="ispH"
                     /locus_tag="VFV93_00135"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5163773.1"
                     /translation="MGVRQIIVAEEMGYCWGVRRALDIIQEAGDPANPVATIGDVIHN
                     PQVVERLRSHGVDTATTIAEAAGRGFTRVAITAHGAGPHMATEAAQHGVELLDTTCPL
                     VTKVQRMATKLAKQGYTVVVYGDSYHPEVKGIMAWSGTTRIFPAKKLADLPWDAPRGS
                     KDPDAKAPPRKVAVVSQTTKNTDEFMKFVNDLTALVSREGGEIRICNTICEPTYERQN
                     ALKQLASQVDLILAIGGKKSSNTARLAEVGNQMGVQSYHIQSAEEIDDSWLEGVENVG
                     ITAGASTPDDVIQQVVDYLTERGLEPPAEGLRKVDLEATPSY"
     gene            4032..4340
                     /locus_tag="VFV93_00140"
     CDS             4032..4340
                     /locus_tag="VFV93_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163774.1"
                     /translation="MARDAVQQVASTDPSGVDVDPASQLFFEFAAPLLMNARNQEEFE
                     TASSLAEFVWAATHFSAAEQALLLDDFITQTQVPEQMIPWLLEVYSELAARKMALVGS
                     "
     gene            complement(4344..4898)
                     /locus_tag="VFV93_00145"
     CDS             complement(4344..4898)
                     /locus_tag="VFV93_00145"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5163775.1"
                     /translation="MATTAARTMSVEEFLQLPDDGCQYELVQGELIEMPGSGFEHSAI
                     NAFLILELGIYNRQHRLGVITSSDGAYVLNRETRTVLVPDVAFTRADRLPPPGERAGV
                     LELVPDLVVEVASPNDSANMISEKVREYLDAGVQLVWVVFPKRRMLQVFTPDQNSRLM
                     YEDDIVDGGDVLPGFELRVGAIFE"
     gene            complement(5071..>5472)
                     /locus_tag="VFV93_00150"
     CDS             complement(5071..>5472)
                     /locus_tag="VFV93_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163776.1"
                     /translation="ADEMIDLATLTGAKVIAIGSESVAVFSNDDDFARRVVAAGTEAG
                     ELFWHMPLWDALKKQIKSEIADMKNTGGRPGGAITAALLLAEFAEGKPWVHLDIAGAA
                     WADSARDYTPKGPTGIGVMALVNYLEAKAAG"
BASE COUNT         1006 a         1787 c         1707 g          972 t
ORIGIN      
        1 acagcgccgt tcgcctgtcc gaaccgccca acgcttcaag gccaagcgat ccttcgctgc
       61 ggctcaggat gacaaggtga gcggctcggt cctcacggac gccgttcgcc tgggaacata
      121 cgccgtaccc caccccccga atcccgaatc ccgaatcccg cccttacgcg taccgccgct
      181 ccgcctccgg gccggtgatc gattcgaggc ggtccttgct gatggagagg tggacgacgg
      241 cgccgtcggc ggtcttgacg aggtgagcgg ggatgtagtg ctcgtggtgg cggaagaagc
      301 cctgctcgac gcggaagccg ctgacccgcc cccgctcatc ggtcgccatc tcggtgatgc
      361 ggccgacttc ctcgttgttg ctatcgacga cgagcgtgcc ttcggagagg atgacggcgt
      421 tggggttccg ctccttgacc tcctcgtcga tcagcaggtc ggtcgcgtag actgacgtgg
      481 ccggccagat gtagccgtag ttgccgtagt agccggccgg cggccagacg tactcggcat
      541 ccatgtactc cgtcagttgc tcgacatcgt gcttgctgag gttgatgtgg acacctttgt
      601 cgtcaatgct ggtgacggcc gtggccggca ggatcttgtc ggtcgggaag aacagcccct
      661 tgcgaacgat gatcgactcg atctccccgt tgcccggctc gacgaccagg ctgtcaacga
      721 caccgagctt ctcgccgtcg ttcccgtaga tatccgcacc aagttcaatg ttcacgttcg
      781 tgtgcccctc tatgcaggtc ggaatgcgac cggacattcg ccggttcgcc agcgtatgtc
      841 aacgcagaac gtatacctcg attgccgcag aacttttgcc agtccccaat atcaacatgt
      901 atcatccgac ctgataccca gcgcatgtgg acctgtccgt gagtccctga gtgcgttttc
      961 gtgataacgg agagatgatc cggcagttga tgttgcctgc ggcacccatg ccgctcgacc
     1021 cccgaccagc gttggtcctg ttcgacctcg accggacgat cgccgacttt gacggcgcac
     1081 ggctggcgcg gacgcagtac gccttcgagc cacacttcgt cgaacccgat ctgctcgacc
     1141 gggccgttgc cgatgctgtc gcccacgcca gcgagggatc ggaccacttc gagcaggtgc
     1201 tggccggata cggcatccgc gagccggagc agatcgagct ggcccgccag cgctacatcg
     1261 aggaccgcta tcgcgggctg cgcctgttcg atgatgcgtt ggaggtcatc gctgccgtta
     1321 gcctggtcgc ggccgtcggt ctggtgacga atggtccgtc ggcgatccag cggccaaaga
     1381 tcgagctgct ggcgatcgag gagcactttc cgctcatcat cgtctccgaa gaagtcggtg
     1441 tctggaaacc agatcccggc atcttcgcga ttgcgcttga gcgggccggg gttgcggcgg
     1501 cgaacgcgat ctacgtcggc gattctgctg agcacgatat tcccggcgcg cacgcagctg
     1561 gactgcgagc catctgggtc aaccggctag ggattgactg gccgggcgcg ctgcccccgg
     1621 atgccgaaat tcggaatctg taccaactgc taccgctcct cggtatcgac cgggaggacc
     1681 gttgatgagt cgccagccac gcgacagaac gtcatccggg atgagcaggg gaacggtcgt
     1741 gatctgggct gcggctgcca tttttctatt gggcgccatt ttctttgcag cccgtgacga
     1801 tggtgatgcc aacgaaacag gcactacccc gatcgtgtct ggcgcgggcg gcgtcgcttc
     1861 aaccacaacg gccaccagct ctggcgagca gacgaccggt aatccaaacg cgaccgagtc
     1921 ggccaccgga aacgcgacgc cggacagcga cggatcgatc ccgcccacgc agcagccggt
     1981 gaccgagtca cccagcttcc ctcccaatga actcggtcag attccgattc tcgaatatca
     2041 ccacctcggc ccggagccgg agcagttcgt gcggaccccg gaccagttcc ggggtgacct
     2101 gcaatggctc tacgagcatg gcttccacgt cgtcagcctg cacgacatcc tgaccgacaa
     2161 catcaacatc ccggccggca agcgcccggt cgcgctgacg ttcgacgaca gcccggccag
     2221 ccagttctcg ctgatcccgc tcagcgacgg ccggctggcg atcgacccca attgcgttgt
     2281 cggcatcctg gagcacttct tcatccagca ccccgatttc ggccggggcg gccattttgc
     2341 catcctgcca cagatgctgt tcgactgggc gccggtcgag gaccgcagcg atcaggctcc
     2401 gtacgccaag gagaagctgg agtggctggt cgcgaacggc tacgagatcg gcaaccacac
     2461 ctacgaccac gtcgacctct cgaccttgac caacgaccag atcatgtacc agctcgccga
     2521 ggacaacatc gccatccacg agctcgttcc cgacgtgccg gtcgaggtcg tgacgctgcc
     2581 gtacggcatg tacccggccg ggggtgacga tacgctgttt cgtggctttg actataaggg
     2641 ccagcactac gccttcacgg ccgcgctgct ggtcggcgcg aacccgaccg tctcgccgct
     2701 ctcgaccgag tacgacccgt attcgaccgc cagaatccag gcgttcgatg aggagctgga
     2761 caaatggttc ggggtcttca ccgaccagcc gggtatactt tatgtcagcg atggcgaccc
     2821 caacacggtc accgtcccca aggatctaca cccctggctc gtcgatacgc tcgacatgtc
     2881 gaagatcggc aaccgggagt tggtgcggta ttgagacgag ttgaaagcgc tgagtgctga
     2941 gtagaaagta cggagagggt gaggctgcgg acgtcgcctc gatgcggacg gcactcagca
     3001 cccagcactc agcactcagc acttgtaact aggagttttg atgggcgtcc gacaaatcat
     3061 cgtggctgaa gagatgggct actgctgggg ggtccggcgg gcgctggaca tcatccagga
     3121 ggcgggcgac ccggccaacc cggtcgcgac gatcggtgat gtgatccaca atccccaggt
     3181 cgtcgaacgg ctgcgcagcc acggcgtcga taccgcgacg acgatcgccg aagcggccgg
     3241 gcgcggcttc acccgcgtgg cgatcaccgc ccacggagcc gggccgcata tggcgaccga
     3301 ggccgcccag catggcgtcg aactgctcga cacgacctgc ccgctcgtca ctaaggtcca
     3361 gcggatggcg accaagctcg ccaagcaggg ctacaccgtc gtcgtctacg gcgattccta
     3421 ccatcccgag gtcaagggca tcatggcctg gtcggggacg acgcgcatct tcccggccaa
     3481 gaagctggcc gacctgccct gggacgcgcc gcgcggcagc aaggaccccg acgcgaaggc
     3541 gccgccccgc aaggtggccg tcgtctcgca gacgaccaag aacaccgacg agttcatgaa
     3601 gttcgtcaat gacctgacgg cgctcgtctc gcgcgagggc ggcgagatcc gcatctgcaa
     3661 cacgatctgc gagccgacct acgagcggca gaacgcgctc aagcagctcg ccagccaggt
     3721 cgacctgatc ctggcgatcg gtggcaagaa gtcgagcaac acggcccggc tggccgaggt
     3781 cggcaaccag atgggtgtcc agagctacca catccagagc gccgaggaga tcgacgacag
     3841 ttggctggaa ggcgtcgaga acgtcggcat cacggccggc gcatcgaccc cggacgatgt
     3901 catccagcaa gtggtcgatt acctgaccga acgggggctg gagccgccgg cagaagggct
     3961 gcgtaaggtt gacctcgaag cgaccccatc gtactgatcg caagctccgc aacgcgcgcg
     4021 actcagtcgc gatggcgcgc gatgccgtcc agcaggtcgc ctcgaccgac ccgagcggcg
     4081 tcgatgtcga tccggccagc cagctcttct tcgagtttgc cgcgccactg ctgatgaacg
     4141 cgcgcaacca ggaggagttc gagacggcct cctcgctggc cgagttcgtc tgggccgcca
     4201 cccacttcag tgccgccgaa caggcgctgc tgctggacga cttcatcacc cagacccagg
     4261 tccccgagca gatgatcccc tggctgctgg aggtctacag cgaactggcc gcccgcaaga
     4321 tggcgctggt tggtagctag cggctactcg aagatcgctc caacgcgcaa ctcgaaaccg
     4381 ggcaggacgt cgccgccgtc gacgatgtca tcctcgtaca tcagcctgct gttctggtcg
     4441 ggggtgaaga cctgcaacat tcggcgcttc ggaaagacga cccaaaccag ttggacgccg
     4501 gcatccaggt attcgcgcac cttctcggag atcatgttcg cgctatcgtt tggagatgcg
     4561 acctcgacaa ccagatcagg cacgagctca aggacgccgg cacgttcgcc gggtggtggc
     4621 agccggtcag cacgcgtgaa ggcgacgtca ggaacgagga ccgtgcgcgt ttctcggttc
     4681 agaacgtacg ctccatcgga actcgtgatg acacccagcc tatgctgtcg gttgtaaatg
     4741 cctagctcca ggatcaagaa ggcgttgata gcactatgct cgaaccccga acccggcatc
     4801 tcgatcaact ctccttgcac aagctcatac tggcagccgt catcggggag ttggagaaac
     4861 tcctcgaccg acatcgtgcg cgctgctgtc gtcgccatcg tcgcaccctc gcttcaggtc
     4921 gtgcgcggta tcgaaccggt tcgagtatca gtgtacgttc ttgacgcaat cgggggcaga
     4981 tgggtctgga tggaggaggc tggtgatttc tcgagcgagt cgggtggggt acagggccgg
     5041 cacaaggcac ggcccctact tctttggcag ctaccccgcc gccttcgcct ccaggtagtt
     5101 gaccagcgcc atgacgccga tgccggttgg gcctttcggg gtgtagtcgc gagcggagtc
     5161 ggcccaggcg gcgccggcga tgtcgaggtg gacccagggc ttgccctcgg cgaactcggc
     5221 cagcagcagc gcggccgtga tcgcgccgcc gggacggccg cccgtgttct tcatgtcggc
     5281 gatctcgctc ttgatctgct tcttgagcgc gtcccagagc ggcatgtgcc agaacagctc
     5341 gccggcctca gtcccggccg cgacgacgcg cctggcgaaa tcgtcgtcgt tggagaagac
     5401 tgccaccgat tcagagccga tcgcgatcac cttcgcgccg gtcagcgtcg ccaggtcgat
     5461 catctcgtcg gc
//
LOCUS       DASUGJ010000005         8636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1667513, whole genome shotgun sequence.
ACCESSION   DASUGJ010000005 DASUGJ010000000
VERSION     DASUGJ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667513"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VFV93_00155"
     CDS             <1..1158
                     /locus_tag="VFV93_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX5163777.1"
                     /translation="ERVRLRELGVSIGSYPTGTWNAITDVAGVEVGQSTVAWGDGSEP
                     LGSGPARTGVTAIWPQREGLLLQPVAAGFFALSGTGEMTARSEIEELGRINTPIVLTN
                     TMSVGLAYDAVCRYLARQDPELGNQHGVLIPVVAECDDEYLNDVRGFHVTHEHVDRAL
                     DAARTGPVDEGCVGSGTGMICFGYKGGIGTSSRRLPEDLGGWTVGVLTMTNFGRRNRL
                     TFAGVLLGRDSGFGIREPGSGMRGETPPNPESRTPNPRDRVDGSCIVIVATDAPLDGR
                     QLARLAKRAALGLGRTGSAGENGSGELLMAFSTTYRPQSGPGTTTQTVIDGESVNDLF
                     AATIDATEEAVLNSLCMATTTTGRYGRVVEALPLDRVREALARAGRIGAGL"
     gene            1240..2079
                     /locus_tag="VFV93_00160"
     CDS             1240..2079
                     /locus_tag="VFV93_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5163778.1"
                     /translation="MIAVTDLTKRYGQVRAVDGISFDVNAGEIFGLLGHNGAGKTTTI
                     RMLTGRARPTSGAATIAGYDVVEDLPRIKPLINLVFEEPNLYTRLSGRDNLRFFCDLY
                     GVPASRADGLLAMVGLDEEAAGRSVKGYSSGMQQRLLIARALINQPRVLFLDEPTRGL
                     DPASARDVRGIILDLARSGTTIFLTTHYMEEADELCDRVAFLAQGKIVALDSPRELKL
                     RYGQRSASVLLADRTTHTLQLDDPADADQLESWMRDGNVLTIHSHEGTLEDVFIALAG
                     RSL"
     gene            2109..3191
                     /locus_tag="VFV93_00165"
     CDS             2109..3191
                     /locus_tag="VFV93_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5163779.1"
                     /translation="MRLSRILTIYRKDVRDAIRDARVLMAIIVPFGLGIFYNLVMDDD
                     NTTTEATIVWAGDSTSQLPDQLVKTTEGVVDVTLDQVVDEAAVREKIANEDADIGLVI
                     PDGFDQQIASGTQPSLTVIRPQDPSIGSDYVAASLAPVLRSMAGQDVPAQLDIQSVEE
                     QDSSNIITRVGLRKYFVLASIIMLVGMIAMLAVPVILADEADKHTLDALVMVASYGEV
                     VTAKALFGITYIVIGVPLLLAITGIMPVDTLLFFGGLAAFSIALVGFGLLLGGLFTAS
                     QLNTWGGLLLLPVLFPAFVTGLPVPRALELIALAIPTSHATRICLNAISGEAIFDQLW
                     LSFVVIALWALAGYGLLLWRLSRREA"
     gene            complement(3199..3822)
                     /locus_tag="VFV93_00170"
     CDS             complement(3199..3822)
                     /locus_tag="VFV93_00170"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX5163780.1"
                     /translation="MSTENSQTLPSRQRTHGDAAAARRWKKWVILIVIVIAIAAIGNA
                     YLVLRRPNLPDEVDGVTVYNNLSNAVVAGPVDYDIHPPVGGPHAALPQLCGFYRVPVA
                     DENIVASLATGAVWIAHQPGLSGDDYETLLDIVHGQYDVLLTPYPGLQHKFVLTAWGR
                     QLALDDVHDSRVDLFVNVYQNRAWAPNVDDSCAEGLGLPLPTNTTGR"
     gene            complement(3906..5672)
                     /locus_tag="VFV93_00175"
     CDS             complement(3906..5672)
                     /locus_tag="VFV93_00175"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5163781.1"
                     /translation="MTTALTDHEQALLEDVSADSLMNSTRAIAQWVRLSGTADEAKAF
                     DWVEQTLQGYGLETTRYQHPGLVSWPESASLVITDAGGMQQTIRCSTHAFATSTPGGG
                     LEGELVAVGSGSAAELGDVDLEGRIALADGIVAPNRHLAIDDSAAVASIWIAGTYLHE
                     RALSPVWGTPTPETAGLLPTKPSVSVTKEGGDQIKAALQRGPVRVRLETEVYRNWNQL
                     PLLVANLRPARDDDDSFVLFSGHVDSWYYGAMDNGTANATMIEVARVLAGHRDEMKRG
                     LRLAFWSGHSHARYAGSAWYADNHWQELHDHCVAHINVDSVGGNGATILSQGNSMAEL
                     REFVSDAIEPVAGQRLDPRRYGRSGDQSFWGHGIPAALMSLSEQPPENADPVLLALHH
                     QISGGASKAGGLGSWWHTPEDTVDKIDPAFLERDAQIYTLLLHRLCTTSILPLDYSAV
                     LDELRQNLAGLAATSRGFELAPVHAQISALASALDRLRDAGSRAGEGGAAVINDALMA
                     VGRALIPVDYTASGMFDHDLAVPTRPLPGLAAATRLAELDPDSDEYHFLHTRLVRERN
                     RLVHGLVEATRAADAGIAALSS"
     gene            5919..7022
                     /locus_tag="VFV93_00180"
     CDS             5919..7022
                     /locus_tag="VFV93_00180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5163782.1"
                     /translation="MALSGGLPSGFLVTRMCRICIVSDDLAGPPDEGVRKFTVAISAA
                     LGRTHDVAVLTTSQASPVAGVRSIPASKTFLSRPLRAAIRQIEPDVLIYATHRSATYF
                     SFIRARILAAYAPSAQIVLLGLQTRRHRWWQRQLIRLIRPDLVIVQSSANQEYLEGIG
                     CRAAVIHSGVDAETFQPVDDERRHALRVQYGITGESPVVLHVGHLQAGRGIAILGERV
                     RSGGCQVLLVASSSTTQEEAVRRQLEQAGVRIVSEYLPRIEELYQLADCYVFPVATTD
                     NAIEVPLSVLEALACDLPVVTTRFGGLPAMFPGPSHPGLVFVATPAELVSETLRLCRQ
                     RVSGLRPLSLPYSWEAAANGLLARAIEAQRRVA"
     gene            7052..7360
                     /locus_tag="VFV93_00185"
     CDS             7052..7360
                     /locus_tag="VFV93_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163783.1"
                     /translation="MTDLLTTAPALVTGRCGLVNVGSGRLTIFLEVARHFATLTDHTP
                     EIPALVDKPVGVQARFADTGRLTRPLDRTPAILLGDGFGRVLRAAEERASVAQRVTVA
                     "
     gene            7364..>8636
                     /locus_tag="VFV93_00190"
     CDS             7364..>8636
                     /locus_tag="VFV93_00190"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5163784.1"
                     /translation="MAAMIADAGPTRIEHPSRPSQPAQLMLDLLRDGEHAPPRPAEQI
                     EAFLDWRRRNKIALLVLAGRCPLWLAADPTFAAALDAEGARYQTQRDEYLRVRDAWQA
                     RGIDCLMIKPAGNPPSFPHTSDNIDILVRPADGLRARETLRQLGYVEIRNVEEPQKFL
                     FRRFHGGRCVLAIHVHERIAWFVGFLDDARVWERSAPAPDDPAVRIPSPEDAILINLA
                     HACYENKELRLVEVLRVRHALTAAGESLDWHYMDEIATGRGWRDGLAFMLLVHAYAEQ
                     SLFGETLIPDTVLTRCEETLAGDRAMLNRLDEIQAAELNDLPLDLSYLFCKRLYYRKI
                     LRDPASDRQRRLRDVAATLIWGVKLKSGLRPQPAMVVSISGPDGSGKTAHAKHLVAAI
                     RLCELRANDVWYRGGSSNLFRLLSHRPRHTSR"
BASE COUNT         1418 a         2979 c         2808 g         1431 t
ORIGIN      
        1 gaacgggtcc gtcttcggga actcggtgtc agcatcggca gctacccgac cggtacgtgg
       61 aacgcgatca ctgatgtggc cggcgtcgag gtcggccagt cgaccgtcgc gtggggcgac
      121 ggcagcgagc cgctcggcag cggcccggcc cggaccggcg tgaccgccat ctggccccag
      181 cgcgaggggc tgctgctcca gccggtcgcg gccggcttct tcgccctctc cggaaccggc
      241 gagatgacgg cccgcagcga gatcgaggag ctcggccgga tcaacacgcc cattgtcctg
      301 accaacacga tgagcgtcgg gctggcctac gacgccgtct gccgttacct cgcccgccag
      361 gacccggagc ttggcaacca gcacggcgtc ctgattccgg tcgtcgccga gtgcgacgac
      421 gagtacctga acgatgtccg cggcttccac gtcacccacg aacacgtcga cagagcgctg
      481 gatgcggcgc ggaccggccc cgttgacgaa ggctgcgttg gcagcggtac cggcatgatc
      541 tgcttcggct acaagggcgg catcggcacc tccagccgca ggctgccgga agacctcggc
      601 ggctggacgg tcggcgtcct gacgatgacc aacttcggcc gccgcaaccg gctgacgttc
      661 gcgggagtac tgttgggacg ggattcgggg ttcgggattc gggaaccggg gtccggaatg
      721 cgaggtgaaa cccctccgaa tcccgaatcc cgaaccccga atccccgcga tcgcgtcgac
      781 ggctcgtgca tcgtgatcgt cgcgaccgac gcgccgctgg atggccggca gctggctcgg
      841 ctggcgaagc gggccgcgct ggggctgggg cggaccggct cggccggcga gaacggcagc
      901 ggcgagctgc tgatggcgtt ctcgacaacc taccggccgc agtccgggcc gggcacgacg
      961 acccagaccg tcatcgacgg agagagcgtc aacgatctct tcgcggcaac catcgacgcg
     1021 accgaggaag ccgtcctcaa cagcctctgc atggcgacga cgacgaccgg ccgttacgga
     1081 cgcgtcgtcg aggcactgcc gctcgaccgg gtccgcgagg cgcttgcacg agccggccgg
     1141 atcggggcgg gcttgtagac ggctggcacg catcttccgc agacttgcac gagacgctcg
     1201 atccgcacga aagggaccgg cttgcaggag actggaacga tgatcgctgt cacggacctg
     1261 accaagcgct atggccaggt ccgggccgtc gacggcatct cgttcgatgt gaacgccggt
     1321 gagatcttcg gactgctcgg gcacaacggg gccggcaaga cgacgacgat ccgaatgctg
     1381 actggccgag cgcgccccac ttccggcgcg gcgacgatcg ccggctacga tgtcgttgag
     1441 gacctaccgc ggatcaagcc gctgatcaac ctcgtcttcg aggaaccgaa cctctatacc
     1501 cgcctctcgg gccgcgataa cctgcgcttc ttctgcgacc tctatggcgt accggccagc
     1561 cgggcggacg ggctgctggc gatggtcggg ctggacgagg aagcggccgg ccgctcggtc
     1621 aagggttatt cgagcggcat gcagcagcgg ctgctgatcg cgcgggcact gatcaaccag
     1681 ccgagagtgc tctttctgga cgagccgaca cgcgggctgg atcccgcctc cgcccgcgac
     1741 gtgcgcggca tcatcctcga tctggcgcgc tccggcacga cgatcttcct gacgacccac
     1801 tacatggaag aggcagacga gctctgcgac cgggtcgcgt tcctcgccca ggggaagatc
     1861 gtcgcgctcg actcgccccg cgagctgaag ctgcgctacg gccagcgatc ggccagcgtc
     1921 ctgctggcgg atcggacgac ccacacgctc cagctcgacg atccggccga cgcggaccag
     1981 cttgagagct ggatgcggga cggcaacgtc ctgacgatcc actcacacga gggcacgctc
     2041 gaagatgtct tcatcgccct cgccgggcgc tcgctgtaac aacgaaggac tggaatggaa
     2101 cccgccggat gcgactgagc cgaattctga cgatctaccg caaggacgtg cgtgacgcga
     2161 tccgcgatgc ccgcgtcctg atggcgatca tcgtcccatt cgggctcggc atcttctaca
     2221 acctcgtcat ggacgacgac aacacgacaa ctgaggcgac gatcgtctgg gccggcgaca
     2281 gcacatcaca gttgcccgac caactcgtca agacgaccga aggcgtcgtc gatgtcacgc
     2341 tcgatcaggt ggtcgacgag gcagccgtcc gcgagaagat cgcgaacgag gacgccgaca
     2401 tcggcctcgt catcccggac ggctttgacc agcagatcgc cagcggcacg cagcccagtc
     2461 tgaccgtcat ccgcccccag gacccatcaa tcggcagcga ctacgtcgcc gcctcgctcg
     2521 cgccggttct gcggtcaatg gccggtcagg acgtaccggc ccaactcgac atccagtcgg
     2581 tcgaggagca ggattcgagc aacatcatca cgcgggtcgg cctgcgcaag tacttcgtgc
     2641 tggcgtcgat catcatgctg gttggcatga tcgcgatgct ggccgtcccg gtcatcctgg
     2701 ccgacgaagc cgacaagcac acgttggacg cgctcgtgat ggtcgcatcc tacggcgagg
     2761 tcgtcaccgc caaggcgctg ttcggcatca cctacatcgt gattggcgtc ccgctgctgc
     2821 tggccattac cggcatcatg ccggtggaca cgctgctctt cttcggcggc ctggcggcgt
     2881 tcagcatcgc gctggtcggc ttcgggctgc tgctcggcgg gctgttcacg gcctcacagc
     2941 tcaacacctg ggggggcctg ctgctgctgc cggttctctt tccggccttc gtcaccggcc
     3001 tgccggtgcc acgggcgctg gaactgatcg cgctggcaat cccgaccagc cacgcgacca
     3061 ggatctgcct caacgccatc tccggcgagg cgatcttcga tcagctctgg ctgtcattcg
     3121 tcgtcatcgc cctctgggcg ctagccggct acggcctgct gctctggcgc ctctcccgcc
     3181 gcgaagcctg atgcggcgct agcgcccggt ggtattcgtc gggagcggca ggccgaggcc
     3241 ctcggcgcat gaatcgtcga cgttcggtgc ccaggcgcgg ttctggtaga cgtttacgaa
     3301 cagatcgacg cgcgaatcgt ggacgtcgtc gagcgccagc tgccggcccc aggccgtcag
     3361 cacgaacttg tgctgcagcc cgggatacgg cgtcagcagc acgtcgtact ggccgtggac
     3421 gatgtcaagc agcgtctcgt agtcgtcacc ggacaggccg ggctggtgtg cgatccagac
     3481 ggcgccggtc gccagcgagg cgacgatgtt ctcgtccgcg accggcacgc ggtagaagcc
     3541 gcacagctgc ggcagggccg cgtgcggccc gcccaccggc gggtggatgt cgtagtcaac
     3601 cggccccgca accaccgcgt tgctgagatt gttgtagacc gtcacgccgt ccacctcatc
     3661 gggaaggttc ggccggcgca acaccaggta agcgttgccg atcgcggcga tggcgatgac
     3721 gatgacgatc agaatgaccc atttcttcca gcggcgggcg gcagcggcgt cgccatgggt
     3781 ccgttggcgg gacggaagcg tctgggagtt ctcggtgctc atgcctgctc gatccgggct
     3841 caccggccgc gccatcgcga ccgcccggaa gcgtaccact ccatctcgtc gtccttcgct
     3901 gcggctcagg acgacagggc cgctatcccc gcatccgccg ccctggtcgc ctcgaccagg
     3961 ccatggacga ggcggttgcg ctcgcggacg aggcgagtgt gcaggaagtg gtactcgtcg
     4021 ctatcggggt cgagctctgc cagccgggtt gcagctgcca gccctggcaa gggccgggtc
     4081 gggacagcca gatcgtgatc gaacatgccg ctggcggtgt agtcgacggg aatcaacgcc
     4141 cggccaaccg ccatcagggc gtcgttgatg actgccgcgc cgccctcacc cgcacggcta
     4201 ccggcatccc gcaggcggtc aagcgccgat gcgagcgcgc tgatctgggc gtggaccggg
     4261 gcgagttcga atccacgact ggtcgccgcc agcccagcca ggttctggcg aagttcgtcg
     4321 aggacggcgc tgtaatcgag cggcaggatc gacgtcgtgc agaggcgatg cagcagcagc
     4381 gtgtagatct gcgcgtcccg ttccaggaag gccggatcga tcttgtcgac cgtgtcctcg
     4441 ggcgtgtgcc accacgagcc aaggccgccg gccttgctgg ccccgccgct gatctggtgg
     4501 tgcagcgcca gcaggaccgg gtcggcattc tccggcggct gctccgagag cgacatcagc
     4561 gcggctggga tgccatggcc ccagaacgac tggtcgccgg agcggccata gcgtcgcggg
     4621 tcgagacgct ggccggcaac cggctcgatc gcgtcgctga cgaactcgcg cagttcggcc
     4681 atgctgttgc cctgcgagag gatcgtcgcg ccattgccgc cgaccgaatc gacgttgatg
     4741 tgagcaacgc agtggtcgtg caactcctgc cagtggttgt cggcatacca ggccgaacca
     4801 gcgtagcggg cgtgcgaatg acccgaccag aatgccagcc gcaacccgcg tttcatctcg
     4861 tcgcggtgcc ccgccaggac gcgggcgacc tcgatcatcg tcgcgttggc cgtcccgttg
     4921 tccatcgcgc cgtagtacca cgaatcgacg tggccgctga agaggacgaa gctgtcatcg
     4981 tcgtcccggg cggggcgcag gttcgcgacc agcagcggaa gctggttcca gttgcggtag
     5041 acctccgtct ccagccgtac cctgaccggc ccccgttgga gtgcggcttt gatctgatcg
     5101 ccgccctctt tggtaacgga gacggatggc ttggtcggca gcagaccagc cgtctccggc
     5161 gtcggcgtcc cccagactgg tgagagcgcc cgctcgtgaa ggtacgtccc ggcgatccag
     5221 atcgacgcga ccgccgccga gtcgtcgatc gccagatgcc ggttcggcgc gacgatcccg
     5281 tcggccagcg cgattcggcc ttcgagatcg acgtccccca actcggcagc cgaaccagag
     5341 ccaactgcga ccagctcgcc ctccagccct ccccccggcg tcgaggtcgc gaacgcgtgc
     5401 gtcgagcagc ggatcgtctg ctgcattccg cccgcgtcag tgataaccag cgatgccgat
     5461 tccggccacg agaccagtcc cggatgctgg tagcgggtcg tctccagccc gtacccctgc
     5521 aacgtctgct cgacccagtc gaacgccttc gcctcgtcgg cggtccctga gagacgcacc
     5581 cattgagcga tcgcccgcgt ggaattcatc agcgagtcgg ccgagacatc ctcaaggagc
     5641 gcctgttcgt gatcggtcaa tgctgtcgtc atcctgcatc tccccacacc ggatcgtcgt
     5701 tggcggccat catcgttccg tcgcgccaga ccgtagatgc gccccgcccc gctgtcaagc
     5761 aagtgatgga agagacccct ccgcgtaggg ggtgcgcggc ccggcctcaa cccattttct
     5821 accgatctgt taccaacccc ggcctcggcg ctccatagac tcggactcga ccaattcgtt
     5881 cgtgaagcca tgtctgccgg ggtcgcggga gatgcccgtt ggcgctgtcc ggcgggctgc
     5941 cgtcagggtt tctggtgacg aggatgtgcc gaatctgcat cgtcagcgac gacctggccg
     6001 gaccgcccga cgagggggtc cgcaagttca cggtcgccat ctcggctgct ctcggacgca
     6061 cgcacgacgt tgccgtgctg acgacgagcc aggcgagtcc cgtcgcgggc gtgcgctcga
     6121 tcccggcctc gaagacgttc ctgagccgtc ccttgcgggc ggcgatccgg cagatcgaac
     6181 cggatgtgct gatctacgcc acccaccgct ccgccaccta cttctcgttc atccgcgcgc
     6241 gaattctggc ggcctacgcg ccgtccgccc agatcgtcct gctggggttg cagacacgcc
     6301 ggcaccgctg gtggcagcgg cagctgatcc ggctgatccg acccgatctg gtgatcgtcc
     6361 agtcatccgc gaaccaggaa tatctggaag ggatcggctg ccgggcagcc gtcatccaca
     6421 gcggcgtcga tgccgagacg ttccagccag tcgatgatga gcgccgtcac gccctgcgag
     6481 tccagtacgg gatcacgggc gagagccccg ttgtcctgca cgtcggtcac ctccaggcgg
     6541 gacgcggaat cgccatcctc ggcgagcggg tacgttccgg cggctgtcag gtgctgctgg
     6601 tcgccagctc cagcacgacg caggaggaag cagtccgccg gcagcttgag caggccggcg
     6661 tcaggatcgt gtccgagtac ctgccgcgca tcgaggagct gtatcagttg gcggattgct
     6721 acgtgtttcc agtcgcgaca accgacaacg caatcgaggt gccgctctcg gtcctggagg
     6781 cgctcgcctg cgacctgccg gtcgtgacga cccgtttcgg cggcctgccg gccatgttcc
     6841 ccggcccgtc gcaccccgga ctcgtcttcg tcgcgacacc cgccgaactg gtcagcgaaa
     6901 cgctgcgcct ctgccggcag cgggtgtccg gtctccggcc gctgtcgctg ccctattcgt
     6961 gggaggcggc cgcgaacggc ctcctcgcga gggcgattga agctcagcgg cgcgtcgcat
     7021 gagccgaagc gtatatccga aggagaatcg catgaccgat ctgctgacga cggctcccgc
     7081 gctcgtgacc ggaagatgcg gcttggtcaa cgtcggttcg ggacgattga caatcttcct
     7141 tgaggtcgca cgccacttcg ctactctgac cgatcacacg ccggagatcc cagcgctggt
     7201 cgacaagcca gtcggtgttc aagctcgctt tgccgacacg ggccggttga cgcgcccgct
     7261 cgaccggacg cccgccatct tgctcggcga tggcttcggc cgtgtcctgc gtgccgcgga
     7321 agaacgtgct tccgttgcgc aacgcgtcac agtggcctga ccgatggctg caatgatcgc
     7381 cgatgccggc ccaacgcgaa tcgaacaccc atcgaggccg agccaacccg cacaactgat
     7441 gctcgatctg ctcagggacg gcgagcacgc gccgccgcgc cctgccgagc agatcgaggc
     7501 gttcctcgac tggcgccgcc gcaacaagat cgcgttgttg gtgctcgccg gccgctgccc
     7561 gctctggctg gcggccgacc ccactttcgc tgccgccctc gacgccgagg gggcccggta
     7621 tcaaacccag cgcgacgaat acctgcgcgt acgggatgcc tggcaggcac gcgggataga
     7681 ctgcctgatg atcaagccag ccggcaaccc gccgtcgttc ccgcacacca gcgacaacat
     7741 cgacattctc gtccgtcccg ccgacggtct ccgcgcccgt gagacgctac ggcaactggg
     7801 atatgtcgag attcgcaacg tcgaggaacc gcagaagttc ctcttccgcc gattccacgg
     7861 cggccggtgc gtcctcgcta tccacgtcca cgagcgcatc gcctggttcg tcggctttct
     7921 ggacgacgcg cgcgtctggg agcgttcggc gccggcgccc gacgatccgg ccgttcggat
     7981 tccctcgccc gaggacgcga tcctgatcaa cctcgcccac gcctgctacg agaacaaaga
     8041 gttgcggctg gtcgaagtgc tacgcgtccg ccatgccctc acagcagccg gcgaaagtct
     8101 cgactggcac tacatggacg agatcgccac ggggcgcggc tggcgcgacg gtctcgcctt
     8161 catgctgctc gtccacgcct acgccgagca atcgctgttc ggtgagacgc tgattccgga
     8221 tacagtgctt acgcgctgcg aggagacgct ggcaggcgac agggcgatgc tcaatcggct
     8281 ggacgagatc caggcagccg aactgaatga tctgccgctc gacctgtcct acctgttctg
     8341 caagcggctc tactaccgca agatcctccg cgatccagcc agcgaccggc aacgccggct
     8401 gcgcgatgtc gcggcgacgt tgatctgggg cgtcaagctg aaatccgggc tacggcctca
     8461 gccagccatg gtcgtctcga tcagcggacc ggatggctcg ggcaagaccg cccacgccaa
     8521 acacctcgtc gccgcgatcc gtctctgcga attgcgcgcc aacgacgtct ggtatcgcgg
     8581 cggctcaagc aacctgtttc gcctgctcag ccatcgaccg cggcacacct ctcgcg
//
LOCUS       DASUGJ010000006         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_139730, whole genome shotgun sequence.
ACCESSION   DASUGJ010000006 DASUGJ010000000
VERSION     DASUGJ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139730"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFV93_00195"
     CDS             complement(<1..575)
                     /locus_tag="VFV93_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX5163785.1"
                     /translation="MYIIEQQDEGLDYRRRFQGLIGVNSKVPIRDRSILRLVYTPGVA
                     EACLEVQRNPESAFELTCRGNTVAIVTDGSDLFGREGGPPEAALPVLEGKSVLFKTFA
                     GIDAFPICLDVHDPATIVETTAALTPTFGAICLDDISAPRSFAIADQLEKACTIPVFS
                     NQHHGTAILVLGALFNALKVTNRTLEDSSVV"
     gene            complement(726..2333)
                     /locus_tag="VFV93_00200"
     CDS             complement(726..2333)
                     /locus_tag="VFV93_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5163786.1"
                     /translation="MDDIVATHQEVMQGQARTAERSQRSDQLLLFGSGALAGLVTVAF
                     MLLLRRVTETDSLLEIVGEALVQLMPMAMFAFLLETFREAAKPLLVVGIVLGMMLAGG
                     GIARMDGGPARAASPGRRFWRGLLVALSIWVPLAIFAVIVVSIGTVAPITNRSVAALA
                     LILLADAIVFVLALYLFFPLLRLALDGQSTRVDSREHVHAPPADLGRRRLIALAATGA
                     VTLASAAWVGRFVTGIRGGSIGGGSNAISEPITPTGNFYLISKNFVDPGVDEGDWRLR
                     LTGLVETPRDIAYADLLAMQRQEQLATLTCISNEIGGELISNSKWTGVRLADILALSG
                     IQPTAAELALYADDGYTESFPLSKALEPTTMLAYLMNDQPLPKRHGFPARLIVPGKYG
                     IKNVKWLTRIDLVSGNFEGYWQQRGWTDDATIQTMSRFDVPGARAIVPFGPVELAGIA
                     FAGDRGISAVEISPGGSTWLPVDTIERVAPLSWVIWRFTWNPPKSGTYTLRVRATDGT
                     GAVQTSDRRDPIPDGATGYHKLDIGVT"
BASE COUNT          432 a          827 c          761 g          409 t
ORIGIN      
        1 atgacgacgc tactgtcctc cagcgtccgg ttggtcacct tgagcgcgtt gaacaacgcc
       61 ccaaggacga gaatcgccgt tccgtggtgc tggttggaga agaccgggat cgtacaggcc
      121 ttttcaagct ggtcagcgat cgcgaacgag cggggcgcgc tgatgtcatc gaggcagatc
      181 gcgccgaagg tcggcgtcaa cgcggctgtc gtttcgacga tcgtcgcggg atcatgcacg
      241 tcgaggcaga tcgggaaggc gtcgatgccg gcgaaggtct tgaagagaac gctcttgcct
      301 tccagaaccg gcagcgccgc ttccggcggc ccgccctcac gcccaaacag gtccgagccg
      361 tcggtgacaa tcgcgaccgt gttgccccgg caggtcagct caaacgcgct ctccgggttg
      421 cgctggacct ccaggcaggc ttcggcgacg ccgggcgtat agaccagccg caggatcgac
      481 cggtcgcgga tgggaacctt gctgttgact ccgatcaatc cctgaaatcg tcgccggtag
      541 tccagccctt cgtcctgttg ttcgatgatg tacacgcttg ctgtgacgtc ccactctgcg
      601 ccctcgcggg cgattcaatc gctactcatc tgtccatctg cgattgtacg tcacgaccgg
      661 tcgcggcgtt gtcggcccaa cgacaccaaa agtgtgaact tggctctcga cgctcaagtt
      721 cgggactacg tcacaccgat gtcgagcttg tggtagccgg tcgcgccatc tgggatcgga
      781 tcgcggcggt cgctggtctg gaccgcgccg gtgccgtcgg tagcgcggac acgcagcgtg
      841 taggtgccgg atttgggcgg gttccacgtg aaacgccaga tcacccaact gagcggagcg
      901 acccgctcga tcgtatcgac cggcagccac gtgctgccgc ctgggctgat ctcaacggcg
      961 ctgatgccgc ggtcgccagc aaaggcgatc ccggccagct cgaccggccc gaacggcacg
     1021 atcgcccgcg cgcccggcac atcgaagcgc gacatcgtct ggatcgttgc gtcgtcggtc
     1081 cagccgcgct gctgccagta gccctcgaag ttgccgctga cgaggtcgat ccgcgtcagc
     1141 cacttgacgt tcttgatgcc atacttcccc ggcacaatca gccgggccgg gaagccgtgc
     1201 cgcttcggga gcggctggtc gttcatcagg taggctagca tcgtcgtcgg ttccaacgcc
     1261 ttcgagagcg ggaaggactc ggtgtagccg tcgtctgcgt agagcgccag ctcggctgcg
     1321 gttggctgaa taccggacag tgccaggatg tcggccagcc gaacgccggt ccacttggag
     1381 ttggagatca actcgccgcc gatctcgttc gagatgcagg tcagcgtcgc cagttgctcc
     1441 tgccgctgca tcgccagcaa atcggcgtat gcgatgtcac gcggcgtctc gaccagcccg
     1501 gtcagccgca gccgccagtc gccctcatcg acgcccggat cgacgaagtt cttcgagatc
     1561 aggtagaagt tgccggtcgg tgtgatcggc tcggagatgg cgttgctgcc gccaccgatc
     1621 gatccgccgc gaatgcccgt cacgaagcgc ccaacccagg cggccgacgc cagcgtgacg
     1681 gcccccgtcg ccgccagcgc gatcaaccgt cgccggccga ggtcggcggg cggcgcgtgg
     1741 acgtgctccc gggagtccac ccgggtggac tgtccgtcga gggccagccg tagcagggga
     1801 aagaacagat agagcgccag cacgaagacg attgcatcag ccagcaggat cagcgcaagg
     1861 gcggcgacgc tgcggttcgt gattggcgcg accgtcccga tcgagacaac aatcacggcg
     1921 aagatcgcca gcggcaccca gatcgagagc gccaccagca gcccgcgcca gaagcgcctg
     1981 ccaggcgagg ccgcccgtgc cggaccgccg tccatccgcg caatgccgcc gccggccagc
     2041 atcatcccca gcacgatccc gacaaccagc agcggcttgg ccgcctcgcg aaacgtctcc
     2101 agcaggaagg caaacatcgc catcggcatc agctgcacca acgcctcgcc gacgatctcc
     2161 agcagcgagt cagtctcggt cacccgccgc agcaacagca tgaaggcaac cgtcaccaga
     2221 ccagccagcg cgcctgatcc gaacagcagc agttgatcgc tgcgctggct tcgctctgcc
     2281 gttcgcgcct gcccttgcat cacttcctga tgggtcgcca cgatgtcgtc catcccgttg
     2341 atcgccggaa ccgtcgttcg tctgccttca acctaccacc ggtcgcttac gcagatatga
     2401 cgctactgtc cggaacgcct tcaccgccg
//
LOCUS       DASUGJ010000007        14900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_973460, whole genome shotgun sequence.
ACCESSION   DASUGJ010000007 DASUGJ010000000
VERSION     DASUGJ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14900
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973460"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..2799)
                     /gene="polA"
                     /locus_tag="VFV93_00205"
     CDS             complement(37..2799)
                     /gene="polA"
                     /locus_tag="VFV93_00205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX5163787.1"
                     /translation="MAGTTVNGQNIANGRKRLVLVDGYGLAFRAFHALPLSMSTAAGE
                     LTNATFGFASMLLDVLRAHDPECILITFDVGKSFRHEQFQDYKAHRAPMAEEMRGQMQ
                     RIREVISSLNIPIYEADGFEADDVIGTLARQASEHGMLAMVVTGDSDLLQLVNDNVIA
                     ILPGTQRFGDYRIYDRQAVIDRYGFGPERLAEYKALVGDKSDNIPGVPGIGEKTAKGL
                     IEKYPSIEEILAHVDEITPTRARNSLAENADLAYQCRELATIVTNVPVELEIERCTVG
                     DYDREKVVDLFRALEFRSLVNKLPDSMHQPAHAEVVASSQQAEPVTVRDRKALEDLAA
                     EIAAAGMISLDVETDGTSPITSTLVGIAIATAEDRAYYIPVMHASEPVADSDDVRATV
                     GPALAAHKNVVTHHGKFDIALMQRHGYPDITIKFDTMLAAYLLGENGIGLKDLAFRYL
                     GWEMTPITDLIGTGKSQTTMDHVAIDEATKYAGADVESTLRLLPVLKPQLVEREQMSL
                     LEEIELPLVPVLIQMELDGIAIDTDVLAEIDADVSQQIAEREREIYEAVGHEFNIAST
                     KQLGEVLFEELKLPTGRKTKTGYSVGQEVLDGLRGAHPAVDAILSHRTLKKLKSTYID
                     ALPLQVNPKTGRIHTNYNQTIASTGRLSSTDPNLQNIPIRTEVGRQIRRAFVADNRPQ
                     YRHFEEESVFLSVDYSQMELRIMAHCSGDPALVSAFQEGLDVHTATAAEVFGVPISEV
                     TSAMRSTAKSVNFGIMYGMQAYGLSRDTGMPRDQARKFIEDYMNKFQGVRDYLDRTLR
                     DAVRLGYAESLYGRRRYLPEITTSGPRRQAAERAAINMPLQGTASDIMKLAMIQVADK
                     LKASKLKGEMLLQVHDELLFETPKSELDDLRALVTETMENVSTLSVPLGTEASWGPNW
                     EDMRDF"
     gene            3155..4162
                     /locus_tag="VFV93_00210"
     CDS             3155..4162
                     /locus_tag="VFV93_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5163788.1"
                     /translation="MESVQHISAAVINNVERVIVGKDREVHLVLVALLCKGHVLIEDV
                     PGVGKTVLAKAIARSVGCTFKRIQFTPDLLPSDVTGVSVFNQQLASFEYRPGPIVAQI
                     VLADEINRATPKTQSALLEAMEESQITVDGDTHMLPKPFVVLATENPIEYEGTFPLPE
                     AQLDRFLVRLSLGYPGRASEIDILNRQQYSHPLEDLEQAVDVSELIDAQAAIKQVHVD
                     ALIKEYIVDLVESTRRHDDIYLGASPRGSLALYNTTRAWAALQGRDYVIPDDVKDLAE
                     PTLAHRMIVSPAARMKGIDSRAVVREILTVVPVPGARHVTNAPQAAGAGGGWRGRRNS
                     Q"
     gene            4159..5427
                     /locus_tag="VFV93_00215"
     CDS             4159..5427
                     /locus_tag="VFV93_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5163789.1"
                     /translation="MNALKLIALAIAVFVLAQLNQWSILDKVFFALLGLLVVAKFWSR
                     FSLGGLRLTRDTKTDRAQVGQPLLERIRIENRSRLAKLWVEIIDHSDMPAHRVSRVVH
                     LGANDATRWRVETICARRGRFRIGPMSLRSGDPFGLFPMEKVIPDTHELLVYPATVDL
                     SAFALPVGELPGGTAIQRRTPFVTPNAAGVRDYFPGDAFNRISWSATARAGKLMVKEF
                     ELDPTADIWIAVDLNASAQAGASGMASLPQFNGGDVPLSFWLDSTEEYAVTIAASLSR
                     HFLNQNRNVGLIGSSEQAISIPTDRGGRQMLKILEQLAVARADGSTPFGEILLAEGTQ
                     FTRNSTVIVVTSSTDEEWVQSLIGIVSRHVQCVAVVIEPSTFGAGESSLFVVSSLASV
                     GVPTYLVKYGDDISRALASHAASPSKAVPV"
     gene            5513..8194
                     /locus_tag="VFV93_00220"
     CDS             5513..8194
                     /locus_tag="VFV93_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX5163790.1"
                     /translation="MQRFAIREGWTTVFLTSMIVFISVWSIQRADWADGLGILNLVAL
                     AGLIAGVAVSKNQRSPTIAAHGLAALAGLLVVLYQMTNYLDDRLGTRRQKLDWLWDRG
                     QRWGEQIWNGERTDDLYLFVFFISILTFVLAYSSMWFVLRARWIWAALVFPGLLLFIN
                     LGYSLRVPNGYVALYIFFSLLLLVRFSLLERETIWRRMRIDYPSTLAWRGLWAASYLA
                     IFVLIFGWAFPASARSGSAHDAWLQVDGPWRTVENQFNDWFVGLRGNTSHGVGGFASF
                     SDSFDLGGPLSLSDAPVVLVTGASRSPYLAAHRYSIYTGRGWESEFAVSDDEEQPRDL
                     PPQVELQPGESVAIDPAIVDGRQSRKYTIQMQRPRGSLIFSPETFSSADVGVNLVLPW
                     QTVTDATVDLTAEEITNVPAELMALVQLLQTTDFTLDVPVSEPADDADATASSGSPTV
                     TTETEEATPEATPSATPTPLRAPEPLEITQAKQALAERGILVNYTVELDQETQTWRAT
                     TMTYSGEFPVYDDVEAVYARNGVATGQSYEVTSLETRALSDDLRIAGGDYPQEIRDRY
                     LQLPTTVTERTRQLARDITAGTQTPYDAAKAIESWLRNNITYQEDIEFPPEGADVVDY
                     VLFQSHAGYCEYYASAFVVMMRSLNIPTRMATGFFPTDRDAAAGGFLYRERNAHAWPE
                     VYFPGEGWVQFEPTANRDEISRDPAPASNVGPPPDDPAISGSIGGGLPDDFELQERGG
                     GAFTGGAVVSQSNDTVSRTEWAIRIVTLGLMGLIVLIAFLWLRGMRGLSPAAQLYAKT
                     ARGASWGGVRYEPHMTPHEYTQEVAKAVPGSRGPATYLADVYVRETYGNRPPAQMDLQ
                     RARQAWQRLRGVLMKHFFLRLRPWRTADTTDSDDDLW"
     gene            8221..9135
                     /locus_tag="VFV93_00225"
     CDS             8221..9135
                     /locus_tag="VFV93_00225"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX5163791.1"
                     /translation="MAGVLVIGSSNTDLVCRTTSLPRPGETVAGRSFATFPGGKGANQ
                     AVAAARAGAAVTFVGAHGDDEFGAARLADLVADGINVSRFKLIEGIASGVAAIVVDER
                     GENQIVYVPGANAEISIADAAAALSEVEHRVISLTFEAPFDVVVHAVQHHPSNSLVVL
                     NAAPFDERVGDLLPDVDVLICNEGEASAILGRPVTTATAIDDAIALRSRGAGAAVITL
                     GADGAVAADPGGSWLTPSPKVSAVDTTGAGDAFCGVLSAWLAAGESLRAAVSAGVAAG
                     ALAVTRDGAQPSLPVRDEIVAMLETTGA"
     gene            9152..10105
                     /gene="corA"
                     /locus_tag="VFV93_00230"
     CDS             9152..10105
                     /gene="corA"
                     /locus_tag="VFV93_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX5163792.1"
                     /translation="MFDTSAGRGFRHVTLDDVSPATEGTVAWVDATGATAAEIDAIGE
                     RFSFHPLAIEDARKRQQRPKIDTYGQHLFVVLYALDPPDDTLRLPARELAMFITPQAI
                     VTIHRHDIEELDEAAVRWTEHCNGSLPETSAMLAYTICDSIVDGYFPCLDEFGDQIED
                     LERAMFDGGDSDSLERIFRMKRQLLEMRRVVAPTRDVFNAFTRRELPVLGEASLAYFQ
                     DVYDHVIRTTDTIDAYRDILSSVIDVHLTLVSNSLNQTVRTLTVVSIILMSLALIAGI
                     YGMNFKHMPELSWHYGYFMALGVMVLLAGGLALLFRRINWW"
     gene            complement(10130..11647)
                     /locus_tag="VFV93_00235"
     CDS             complement(10130..11647)
                     /locus_tag="VFV93_00235"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX5163793.1"
                     /translation="MTSHVSNGANRHLVVVSNRLPVVLSMRAGEWDAQAASGGLITAL
                     APVLRNRGGTWIGWPGTSEIDAIRHALDEASLEAGYRLEPVRLPEEIVDGYYHGFANE
                     ILWPLFHDLTSRCNFEPSHWHAYQEANRVFAETIADRTDPGDYIWVHDYHLMLVAEQL
                     RAMNGSRPIGFFLHIPFPAVDIFLKLPWRFQILRALLEYDIVGFQTVRDTQNFIHCVR
                     TLMTGVSIKGRRSSHVVEQPGRRTQVGSFPIGIDFRSFDERARSQAVEQEVRRAHDRL
                     PNIKMILGIDRLDYSKGLPERIEAFQNALRRYPDLQGNVTLIQVVIPSREDVPEYQIQ
                     RAEIERLVGEINGEFTQPGWVPIHYLYRSLDPIELFAYYRMAEVCLVTPLKDGMNLVS
                     KEYCACNVEGDGVLILSEFAGSAAQLFRGAIMVNPHDVEGTADAIHAAILMPLDERRK
                     RMKRLREIVRREDIFWWTDSFLAAAFSRRLDEFPVHEYFVPQADIRAMTTAELEH"
     gene            complement(11644..12477)
                     /gene="otsB"
                     /locus_tag="VFV93_00240"
     CDS             complement(11644..12477)
                     /gene="otsB"
                     /locus_tag="VFV93_00240"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX5163794.1"
                     /translation="MADQINTPVEHDHVPANALVAVAGVADFWEQVCAAPHISLFLDF
                     DGTLAPFQADRLQAFPLPGTVDALNRIIDERLASVAIVSGRPIAEITLLMGDLETTIV
                     GAHGYEIHNPGGVKHVVSIRPEQAAILDAAYDEAFAIFDPARVERKAASVAVHFRGLD
                     PRATSSIQDKLERQWRAASTTDLMEFRPFNGGLELRAAGRTKGTAIKELLAIAPDDSL
                     PIYIGDDDTDEDAFAVLESCGIGIKVGPQDAVTKAAGRVPDCETVRQILIDWPNGRRC
                     T"
     gene            complement(12643..12732)
                     /locus_tag="VFV93_00245"
     tRNA            complement(12643..12732)
                     /locus_tag="VFV93_00245"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12695..12697),aa:Ser,seq:gga)
     gene            complement(12848..12937)
                     /locus_tag="VFV93_00250"
     tRNA            complement(12848..12937)
                     /locus_tag="VFV93_00250"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12901..12903),aa:Ser,seq:cga)
     gene            complement(13032..13104)
                     /locus_tag="VFV93_00255"
     tRNA            complement(13032..13104)
                     /locus_tag="VFV93_00255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13069..13071),aa:Arg,seq:gcg)
     gene            13610..>14900
                     /gene="ilvB"
                     /locus_tag="VFV93_00260"
     CDS             13610..>14900
                     /gene="ilvB"
                     /locus_tag="VFV93_00260"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX5163795.1"
                     /translation="MVATGKLDEPAAAALRRTEPEQAADRPMIGARVLCHALQRERVD
                     TIFGYPGGVVIPLYDVLPEFDIHHVLVRHEQGAGHAADGYARATGKVGVCLATSGPGA
                     TNLVTPIATAMLDSVPLVAITGQVSQGVIGSDAFQEIDITGITLPITKHNFLVRSVHD
                     IGPTIQKAFHLASSGRPGPVLVDIPKDVLLATGELGEPAKLELQGYKPTYTPHRRQIR
                     LAAQEIRNARKPLILAGHGILISGAENELKTLSERAQIPVGLTLLGIGGFPQSHPLCL
                     GMIGMHGFAHANRAIHEADLIIGVGMRFDDRVTGRLSEFAPNARVVHIDIDPAEIGKN
                     VPADVPVVGDARQALGLLAAELEDQQHEAWLGEIAEWRRIPPYRSSAESQTLLPQFVI
                     ERLHDLTEGKAVVTTDVGQHQMWAAQFYKCDQPNQWIS"
BASE COUNT         2575 a         4756 c         4788 g         2781 t
ORIGIN      
        1 gcccataccg gattgagcat tggtattggg gcacagctag aagtcgcgca tatcctccca
       61 gttcgggccc caggaggctt cggtgccgag cgggaccgat agggtgctga cgttctccat
      121 cgtctcggtg acgagggcgc gcaggtcgtc gagttcagat ttcggtgtct cgaagagcaa
      181 ctcatcatgc acctgcagca gcatttcacc cttcagcttc gacgccttga gcttgtcggc
      241 gacctggatc atcgccagct tcatgatgtc ggaagcggtg ccctgtagcg gcatgttgat
      301 ggcggcccgc tcggcggcct ggcggcgcgg gccgctggtc gtgatctccg gcaggtagcg
      361 acggcggcca tagagcgatt cggcgtagcc gaggcgcact gcgtcgcgga gcgtgcggtc
      421 gagatagtca cgaacgccct gaaacttgtt catgtagtct tcgatgaact tgcgcgcctg
      481 atcgcggggc atgccggtat cgcgcgagag accgtaggcc tgcatcccgt acatgatccc
      541 gaagttgacc gacttggccg ttgagcgcat cgccgacgtg acctcggaga tcggcacacc
      601 gaagacttca gccgctgtcg ccgtgtggac gtccagcccc tcctggaagg cactgaccag
      661 cgccgggtct ccgctgcagt gggccatgat gcgaagttcc atctgcgagt aatcgaccga
      721 gaggaagacc gactcctctt cgaagtggcg gtactgtggc cggttgtcgg cgacgaacgc
      781 gcgccggatc tggcggccga cctcggtccg gatcgggatg ttctgcaggt tggggtcggt
      841 ggatgagagc cggccggtcg aggcgatcgt ctggttgtag ttggtgtgga ttcgaccagt
      901 cttcgggttg acctgcaacg gcagcgcgtc aatgtaggtg gacttgagct tcttcagcgt
      961 gcggtgcgag aggatggcat cgacggccgg gtgggcaccg cgcaggccgt ccagcacctc
     1021 ctggccgacc gagtagcccg tcttcgtctt gcggccggtc ggcagcttca gttcctcaaa
     1081 taacacttcg ccgagctgct tggtcgacgc gatgttgaac tcgtggccga ctgcctcgta
     1141 gatctctcgc tcgcgctcag cgatctgctg cgagacatcg gcatcgatct cggccagcac
     1201 gtcggtgtcg atcgcgatcc cgtccagctc catctggatc aggaccggca ccagcggcag
     1261 ctcgatctcc tccagcaacg acatctgctc gcgttcgacc agttgcggct tgaggaccgg
     1321 cagcagccgc agcgttgact cgacatcggc gcccgcatac ttggtcgcct cgtcaatcgc
     1381 gacgtggtcc atcgtcgtct gggacttgcc ggttccaatc aggtcggtga ttggcgtcat
     1441 ctcccagccc aggtagcgga aggcgagatc cttcagcccg atcccgttct cgccgagcag
     1501 gtaggcggcc agcattgtgt cgaacttgat cgtgatgtcc gggtagccgt ggcgttgcat
     1561 cagcgcgatg tcgaacttgc cgtggtgggt gacgacgttc ttgtgggctg ccagcgccgg
     1621 accgaccgtc gcgcgaacgt cgtcgctgtc ggcgaccggc tcgctggcgt gcatgaccgg
     1681 gatgtagtag gcgcgatcct cggccgttgc gatcgcgatc ccgaccaggg ttgacgtaat
     1741 tgggctggtg ccgtccgtct cgacgtcgag cgagatcatc ccagcggcgg cgatctcagc
     1801 tgccagatcc tcaagtgctt tgcggtcgcg aacggtaacc ggctcggcct gctgcgacga
     1861 ggcgacgacc tcggcgtggg ccggctggtg cattgaatca ggcagcttgt tgacgagtga
     1921 gcggaactcc agcgcgcgga acagatcgac gaccttctcg cggtcgtagt cgccgacggt
     1981 gcagcgctcg atctccagct cgaccgggac gttcgtgacg atcgtcgcca gttcccggca
     2041 ctgatacgcc agatcggcgt tctcggccag cgagttgcgg gcgcgggtcg gcgtgatctc
     2101 atcgacgtgg gccaggatct cctcgatcga cgggtatttc tcgatcaacc ccttggcggt
     2161 cttttcgccg atgccgggta cgccgggaat gttgtcggac ttgtcgccga ccaacgcctt
     2221 gtactccgcc agccgttccg ggccgaagcc gtagcggtcg atcacggcct ggcggtcgta
     2281 gatccggtag tcgccgaagc gctgcgtgcc gggcaggatt gcgatcacgt tgtcgttgac
     2341 aagctggagc aggtcgctgt cgccggtgac gaccatcgcc agcatgccgt gctcggatgc
     2401 ctggcgtgcc agcgtgccga tcacatcgtc ggcctcgaag ccgtcggcct cgtagatcgg
     2461 gatgttgagg gaggagatga cctcgcggat gcgctgcatc tggccgcgca tctcctcggc
     2521 catcggcgcg cgatgcgcct tgtagtcctg gaactgctcg tgccggaacg acttaccgac
     2581 gtcgaaggtg atcagaatgc actccggatc gtgggcgcgc aggacatcca gtagcatcga
     2641 ggcaaagccg aatgtcgcgt tcgtcagttc accggccgcc gtcgacatcg acagcggcaa
     2701 cgcgtggaac gcgcggaagg caaggccgta tccgtcgacc aggacgagcc gcttgcgccc
     2761 attcgcgatg ttctgaccgt tcacggtcgt tccagccacc ggccgtctcc tctccgtaca
     2821 actgttctac tgcattatac gtacgcacca ctatgagggt ggggaactga ttccgcgttt
     2881 catacgtact agttgaaaag gtggtcgtgg atttttgcgg caacgcggca ctgtcgtgtg
     2941 cccgctagtt gctataatat cgggaaacat cgcgctgagc cgtggtggcg tggcctggtg
     3001 aacgatgcca aactccgttc ccgccaggcc gctgtttgtt taccgggaac ccttatcccc
     3061 ggtggaccac ctttcgtatt cgtcgacgta acagggaaga tcgcggaatc cggcggatcg
     3121 gtcttcgcat ctgccgccgc ggaaagggaa cacgatggaa tcggtccagc atatctcagc
     3181 ggcggtcatc aacaacgtcg aaagggtgat cgtcggcaag gaccgagagg tccaccttgt
     3241 cctcgtcgcg ttgctctgta aaggtcatgt cctgatcgaa gacgttcccg gtgtcggcaa
     3301 gacggtgctg gcgaaggcga tcgcacgcag cgtcggctgc accttcaagc ggattcagtt
     3361 tacccccgac ctgctgccga gtgatgtcac cggcgtcagc gtcttcaacc agcagctcgc
     3421 cagtttcgag taccggcccg gcccaatcgt ggcccagatc gtcctcgccg acgagatcaa
     3481 ccgcgccacg ccgaagacgc agtcggcgct gcttgaggcg atggaggagt cacagatcac
     3541 cgtcgacggc gatacccaca tgctgccgaa gccgttcgtc gtcctggcga ccgaaaaccc
     3601 gatcgaatat gaaggcacct tcccgctgcc ggaagcgcag ctcgaccgct tcctggtccg
     3661 gctctcgctc ggttatcccg ggcgggccag cgagatcgac atcctcaatc gccagcagta
     3721 ttcgcacccg ctggaggatc tcgaacaggc cgttgatgtg tcggaactga tcgacgccca
     3781 ggctgccatc aagcaggtgc acgtcgatgc tctgatcaag gaatacatcg tcgacctcgt
     3841 cgaatcgact cgccgccacg acgatatcta cctcggcgcg agcccacgtg gctcgctggc
     3901 gctatacaac acgacccgcg cctgggcggc gctacagggc cgcgactatg tgattcctga
     3961 tgatgtcaag gatctcgccg agccgacgct cgcgcaccgc atgatcgtca gcccggccgc
     4021 gagaatgaag ggcatcgact cacgcgcagt cgtccgggag attctgacgg tcgttccggt
     4081 tcccggcgcg cgacatgtga cgaacgctcc gcaggctgcg ggggcgggtg gcggctggcg
     4141 cggtcgccgg aacagccagt gaacgccctt aagctgatcg cgctggcgat cgccgtcttt
     4201 gtcctggctc aactgaacca gtggagcatt ctggacaagg tcttcttcgc gctgctcggc
     4261 ctgctggtcg tcgccaagtt ctggagccgg ttcagcctcg gtggcctgcg gctgacgcgc
     4321 gacacgaaga ccgatcgtgc gcaggtcggc cagccgctgc tggagcgcat ccgaatcgag
     4381 aaccggagtc ggctggcgaa gctgtgggtt gagatcatcg accactcaga catgccggcc
     4441 caccgcgtct cgcgcgtcgt ccatctcgga gcgaacgacg caacccgctg gcgggttgag
     4501 acgatctgcg cccgtcgtgg ccgtttccgg atcgggccga tgtcgcttcg gagcggcgat
     4561 ccattcggcc tgttcccaat ggagaaggtc atccctgaca cgcacgaact gctcgtctac
     4621 ccggcgaccg tcgatctgag cgccttcgcc ctgccggtcg gtgagctacc gggcggcact
     4681 gccatccagc gtcggacgcc gttcgtgacg ccgaacgcgg ccggggtccg tgactacttt
     4741 cccggtgacg ccttcaaccg catctcgtgg tcggcgacgg cgcgggccgg caagctgatg
     4801 gtcaaggagt tcgagctcga cccgacggcc gacatctgga tcgcggtcga tctgaacgcg
     4861 agtgcacagg ccggagccag cgggatggcg tcgctgccgc agttcaacgg tggcgatgta
     4921 ccgctctcgt tctggcttga ctcgaccgag gaatatgccg tgacgattgc ggcatcgcta
     4981 tcgcggcact tcctcaatca gaaccgcaac gtcggcctga tcggtagctc ggagcaggcg
     5041 atctcgatcc caaccgaccg gggtggccgg cagatgctca agatcctcga acagctcgcc
     5101 gtcgcgcggg cagacggatc gacgccgttc ggtgagattc tgctggctga ggggacgcag
     5161 ttcacgcgca acagtactgt tatcgtcgtc acctcctcga ccgacgagga gtgggtccag
     5221 tcgctgatcg ggatcgtctc gcggcacgtc cagtgtgtcg ctgtcgtcat cgagccgagc
     5281 acgttcgggg ctggtgagtc gagcctgttc gttgtctcaa gcctggcgtc ggtcggtgtg
     5341 ccgacctatc tcgtcaagta cggcgacgat atcagccggg cgcttgcatc gcacgcggca
     5401 tcgccgtcga aggccgtgcc ggtctagccc aacccggcta acgattcagc cgtaacggcg
     5461 tttagcgtgc aagccacgat tctgaccagt tgtctcatga ggtgtaggta ccgtgcaacg
     5521 atttgcaatt cgtgaaggct ggacgaccgt ctttctgacg tcgatgatcg tcttcatctc
     5581 ggtctggtcg attcagcgcg ctgactgggc cgacggcctg ggcatactga acctggtcgc
     5641 cctggccggg ctgatcgccg gcgtggccgt ctcgaagaat cagcgtagcc ccacgatcgc
     5701 cgcccacggg ctggccgcgc tggccggtct gctggtcgtc ctctatcaga tgacgaacta
     5761 cctcgacgat cggctcggga cgcggcgtca gaagctggac tggctctggg atcgcggcca
     5821 gcgctggggc gagcagatct ggaacggcga gcggactgac gatctgtacc tgttcgtctt
     5881 ctttatctcg atcctgacgt tcgtgctggc ctacagctcg atgtggttcg tcctgcgcgc
     5941 gcgctggatc tgggccgcgc tcgtcttccc cggcctgctg ctattcatca accttggcta
     6001 ctcgctgcgg gtgccaaacg gctatgtcgc gctctacatc ttcttctcac tgctgctgct
     6061 ggtccgattc tcgctgctgg aacgcgagac gatctggcgc cgcatgcgga tcgactaccc
     6121 gtcgacactg gcctggcgtg gtctttgggc cgcgtcctac ctggcgatct tcgttctgat
     6181 cttcggctgg gcctttccgg ccagcgcacg tagcggctcg gctcacgacg cctggctgca
     6241 ggtcgatggc ccgtggcgca cggtcgagaa tcagttcaac gactggttcg tcggcttgcg
     6301 cggcaacacc tcccacggcg tcggtggctt cgccagcttc tccgacagct tcgatctggg
     6361 tggaccgctc tcgctgagcg atgcaccggt cgtcctcgtc accggagcat cgcgctcgcc
     6421 gtacctggcg gcccatcgct attcgatcta caccggccgc ggctgggagt ccgagttcgc
     6481 cgtctcggat gacgaagagc agccgcgaga cctgccgccg caggtcgagt tgcagcctgg
     6541 tgagtcggtt gcgatcgatc cggccatcgt cgatggacgg cagtcgcgca agtacacgat
     6601 ccagatgcag cgcccgcgcg gcagcctgat cttctcgccg gagacgttct cgagcgcaga
     6661 cgttggcgtc aacctcgtcc tgccctggca aacggtcacc gacgcaacag tcgacctgac
     6721 cgccgaggag atcaccaacg tcccggctga actgatggcg cttgtccagc tactccagac
     6781 gaccgacttc acgctcgacg ttcccgtctc cgagccggcc gacgacgcgg acgcgacggc
     6841 gtcatcaggc tccccaacag tcacaacgga gaccgaggaa gcaacacccg aagcgacgcc
     6901 gtcggccaca ccaacgccgc tccgtgcgcc cgagccgctg gagatcacgc aggccaagca
     6961 ggcgctcgcc gaacggggca tcctcgtcaa ctacaccgtc gagctcgatc aggagacgca
     7021 aacctggcga gcaacgacga tgacctactc gggtgagttc ccggtctacg acgacgtcga
     7081 agccgtctac gcccgcaatg gcgtcgcgac cgggcagtcg tacgaggtaa catcgctgga
     7141 gacccgcgcc ctcagcgacg acctgcgaat cgccggcggt gactacccgc aggagatccg
     7201 cgaccgctac cttcagctgc cgacgaccgt caccgaacgc actcggcaac tggcgagaga
     7261 catcaccgcc ggcacccaga caccctacga tgccgccaag gcgatcgagt cctggctgcg
     7321 caacaacatc acctatcagg aggacatcga atttcctccc gagggcgcgg atgtcgttga
     7381 ctacgtgctg ttccagtcgc acgcggggta ctgcgaatac tacgcctcgg cattcgtcgt
     7441 tatgatgcgc agtctgaaca tcccgacccg gatggcgacc ggcttcttcc cgaccgaccg
     7501 cgacgccgcg gcgggcggct tcctgtaccg cgagcgcaac gcgcacgcct ggccggaggt
     7561 ctacttcccc ggcgagggtt gggtgcagtt tgagccgacc gcgaaccgcg acgagatcag
     7621 ccgcgatccg gcgccggcca gcaacgtcgg accgccgccc gatgatcccg ctatcagcgg
     7681 ttcgatcggt ggtggattgc cggacgactt cgagctgcag gagcgtggag gtggcgcgtt
     7741 caccggtggc gccgtcgtct cacagtcgaa cgacacggtc tcgcgcaccg agtgggcgat
     7801 ccggatcgtg acgctgggtc tgatggggct gatcgtcttg atcgcgttcc tctggctgcg
     7861 cgggatgcgg ggcctcagcc cggcggcgca actctacgcc aagaccgcac gtggggcgag
     7921 ttggggcggc gttcgttacg agccacatat gacgccgcac gagtacactc aggaggtcgc
     7981 caaagctgtt cccggcagcc gtggtccggc cacctatctt gcggatgtct acgtgcgcga
     8041 aacgtatggg aaccggcccc ccgcccagat ggacttgcag cgggcgcggc aggcgtggca
     8101 gcggctgcgg ggtgtgctga tgaagcactt cttcctgcgg ctgcggccat ggcggacggc
     8161 tgacacgacg gattctgacg acgatctctg gtagcgcgaa cgagagcggc aggcgggttg
     8221 atggcaggcg tcctcgtcat cgggagttcc aatacggacc tcgtctgccg tacgaccagc
     8281 ctcccgcggc cgggtgagac cgtcgccggt cgctcgttcg cgaccttccc gggcggaaag
     8341 ggcgcgaatc aggcagtcgc agccgcccgg gccggcgcgg cggtgacgtt tgttggcgct
     8401 catggcgacg acgagttcgg cgcggcccgg ctggccgatc ttgtcgcgga tgggatcaat
     8461 gtcagccgat tcaagctcat cgaggggatc gcgtccggcg tcgccgccat cgtcgttgac
     8521 gagcgcgggg agaaccagat cgtctacgtt cccggcgcaa atgccgagat cagcatcgct
     8581 gatgcggctg cggcgttgtc cgaggtcgag caccgcgtca tcagcctgac cttcgaagcc
     8641 ccattcgacg tcgtcgtcca tgccgttcaa catcatccga gtaacagtct cgtcgtcctg
     8701 aacgcagcgc cgttcgacga acgggtagga gatctgctgc cggatgtcga tgtcctgatt
     8761 tgcaacgaag gcgaagcgtc ggcgattctt ggcaggccgg tgacgacagc gacagccatc
     8821 gacgatgcga ttgccttgcg ttcgcgcggc gctggcgcgg cggtcataac gctcggtgct
     8881 gacggcgccg tagctgccga ccccggcggt agctggctga cgccttcacc caaggtatct
     8941 gccgttgaca ccaccggcgc gggcgacgcc ttttgcggcg tgctgtctgc ctggctggcg
     9001 gccggtgaat cgctgcgggc ggcggtcagc gccggcgttg ctgccggggc gctggccgtg
     9061 acgcgcgatg gcgcacaacc ctcgctaccg gtgcgtgacg agatcgtcgc gatgcttgag
     9121 acgacgggcg cctgatccga tgagcctgcg catgttcgac acgagcgccg ggcgggggtt
     9181 ccgccacgtg acgctcgatg acgtctcgcc ggctaccgaa gggacggtag cctgggtcga
     9241 tgcgaccggc gcgacggcgg ctgagatcga cgcgatcggc gagcgcttca gcttccaccc
     9301 gctggcgatc gaggatgcac gcaagcgcca gcagcgcccg aagatcgaca cgtatgggca
     9361 gcacctgttc gtcgtcctct acgcgctcga ccctccagac gacacgctac gcctaccggc
     9421 ccgcgagctg gcgatgttca tcacaccgca ggcgatcgtg acgattcacc gtcacgatat
     9481 cgaggaactc gacgaagccg ctgtccgttg gactgagcac tgcaacggca gcctgcccga
     9541 gacgtcagcg atgctcgcct acacgatctg cgactcgatc gtcgatggtt actttccttg
     9601 cctggatgag tttggcgatc agatcgaaga cctcgaacgg gcaatgttcg atggtggcga
     9661 ttctgattcg ctggagcgta tctttcgcat gaagcgccaa ctgctcgaaa tgcgccgggt
     9721 cgtcgcgcca actcgggacg tcttcaatgc cttcacccgt cgagagttgc cggtcctcgg
     9781 cgaggcgtcg ctggcctact tccaggatgt ctacgaccac gtcattcgaa caaccgacac
     9841 gatcgacgcc taccgggaca tcctctcgtc ggtcatcgac gtccatctga cgctcgtctc
     9901 caatagcctc aaccagaccg tcaggacgtt gaccgtcgtc agcatcatcc tgatgtcgct
     9961 ggcactgatc gccggaatct acggcatgaa tttcaagcac atgccggagt taagctggca
    10021 ctacgggtac ttcatggcac tcggcgtgat ggttcttctc gccggggggc tggccctgct
    10081 gttccgccgc atcaactggt ggtgaggccg gcgttcacga ctctgctctt cagtgctcca
    10141 gttcggccgt cgtcatcgcg cggatgtcgg cctgcggcac gaaatactcg tggaccggga
    10201 actcatccag ccggcggctg aacgcggcgg ccaggaacga gtccgtccac cagaagatgt
    10261 cctcgcggcg cacgatctca cgcaggcgct tcatgcgctt gcgacgctcg tcgagcggca
    10321 tcaggatggc cgcgtgaatc gcgtcggcgg ttccttcgac atcatgcggg ttgaccatga
    10381 tcgcgccccg gaagagttgg gccgcgctgc cggcgaactc gctcagaatc agaacgccgt
    10441 cgccctcgac gttgcaggcg caatactcct tcgagacgag gttcatcccg tccttcaacg
    10501 gcgtcacgag gcaaacctcg gccatccggt agtaggcaaa cagctcgatc gggtcgagcg
    10561 agcggtacag gtagtggatc ggcacccagc cgggctgggt gaactcgccg ttgatctcgc
    10621 cgaccagccg ttcgatctcg gcgcgctgga tctggtattc gggaacatcc tcgcgactgg
    10681 ggatgacgac ctggatcagc gtcacgttgc cttgcaggtc cgggtagcgg cgcagcgcgt
    10741 tctggaatgc ctcgatgcgc tccggcagcc ccttgctgta gtccagccgg tcgatgccga
    10801 ggatcatctt gatgttcggc agtcggtcgt gggctcgccg gacctcctgc tcgaccgcct
    10861 gcgacctcgc ccgctcgtcg aagctgcgaa agtcgatccc gatcgggaag ctgccgacct
    10921 gggtgcggcg tcccggctgc tcgacgacgt gcgagcttcg ccggcccttg atcgagacgc
    10981 cggtcatcag cgtcctgacg cagtggatga agttctgcgt gtcccggaca gtctggaagc
    11041 cgacgatgtc gtactccagg agcgcccgta ggatctgaaa gcgccacggc agcttcagga
    11101 agatgtcaac ggccggaaac gggatgtgca ggaagaagcc gatcggccgg ctgccgttca
    11161 tcgcacgcaa ttgctccgcg accagcatca gatggtagtc gtggacccag atgtagtcgc
    11221 cgggatcggt tcgatcggcg atcgtctcgg cgaagacccg gttggcctcc tggtaggcgt
    11281 gccagtggga cggctcgaag ttgcagcgcg aggtcaggtc gtgaaacagc ggccagagga
    11341 tctcgttggc gaagccgtgg tagtagccat cgacgatctc ctccggcagc cggaccggct
    11401 caagccgata accggcttcc agcgacgcct cgtccagcgc atggcggatg gcgtcgattt
    11461 cggacgtgcc gggccagccg atccaggtcc ctccgcgatt gcgcagcacc ggcgccagcg
    11521 cggtgatcaa cccaccggat gcagcctggg cgtcccattc gccggcgcgc atcgagagga
    11581 cgaccggcag acgattcgag acgacgacga ggtgcctgtt cgcgccgttg gaaacgtggg
    11641 acgtcacgtg catctccgtc cgttgggcca atcgatcagg atttgccgga ccgtctcgca
    11701 gtccggtacg cgaccggctg ctttcgtcac ggcgtcctgc ggcccaacct tgatgccgat
    11761 accgcagctc tccaggacgg cgaaggcgtc ttcgtcggtg tcgtcgtcgc cgatatagat
    11821 cgggagtgag tcgtcgggag cgatcgcgag cagttccttg atcgcggtcc ccttcgtccg
    11881 tccggcagca cgcaattcca gtccaccatt gaatgggcgg aactccatca ggtccgttgt
    11941 gctggccgcg cgccactgtc gttcgagctt gtcctggatt gaggaggtcg ctcgcggatc
    12001 gagccctcga aaatggacgg cgacgctggc cgctttacgc tcgacgcggg cagggtcgaa
    12061 gatcgcaaac gcttcgtcgt acgctgcatc caggatcgcg gcctgttcgg gccggatcga
    12121 gacgacatgc ttgacgccgc ccggattgtg gatctcgtaa ccgtgcgcgc caacgatcgt
    12181 cgtttcgaga tctcccatca ggagcgtgat ttcggcaatt ggccggccgg aaacgattgc
    12241 cacactggcg agacgctcgt cgatgatccg gttgagcgca tcgacggtcc ctggcagcgg
    12301 aaaggcctgc aagcggtctg cctggaacgg cgccagcgtc ccgtcgaagt cgaggaagag
    12361 cgagatgtgc ggtgccgcgc agacctgctc ccagaagtca gcaacccccg cgacggcgac
    12421 gagcgcgttc gccggcacgt ggtcatgctc gaccggcgtg ttgatctgat cagccacagg
    12481 caggtccgtt tctgaattcg cggaagtcac tacgtcgtca gtggacgtac ccgcaagaag
    12541 catgcgatgc agcaccggcg cagtggcggc caaagccggt gaatggaaat gaacgctcgc
    12601 cagggagcgc tccagtggga cggatgcgtg cagattccgg ggcggaaggg gagggattcg
    12661 aaccctcgag gcgagaggtc ccgcctacgc gctttccagg cgcgcgcact aggccaacta
    12721 tgcgaccctt cctcgtgatg gcttgcgaat agcaatcatc cctcagtccg gcgttgggcc
    12781 agttcagcgt gagctgtctg tatccgttca cctgaatcgt cgctggggga ggaagactgc
    12841 cagcgcctgg cggagaggga gggattcgaa ccctcggtgg cttttacacc acaccgattt
    12901 tcgagatcgg caccatcaac cgctcggaca cctctccgtc cgttagtata gccgaactgc
    12961 tgctacctgc acaaatccgg ctggacgctg gacaacaaaa aaccacggcc atctgaccgt
    13021 ggttcctgag gtacgcccgg agggatttga acccccgacc ttttggtccg caaccaaacg
    13081 ctctatcccc tgagctacgg gcgcgtgcct gaatccgcgc cgccaccggc gcaccacgaa
    13141 tcatagcaga tttggcggtc cggcaccagc gtttggcgag tgtcgtgacg cgggacagat
    13201 ggatatgcaa ttcgacccgc cgcataacca atccgcgtcc ctcatcccac gtcccgaacc
    13261 actacggatt gtgaaatgtg acacagactg cttgacagcg gcgggattgc cccctatcat
    13321 cgattcagtc ggttttgcat gcacgcgcaa ggtgttcctg tgccgcaaat ggtcgcgccg
    13381 agtcgaatgg ttgaggacgt ggtgcttgat gacaccgcgc ttccggctgc gctcggtgta
    13441 gtagcgatca ttattattga cggcattatt ctccttaccg gggagacata agggagcgca
    13501 cgcgaaaccg gccgatagac agaacctcgc gagcctcccc tcaccgggag gctcgtttcg
    13561 tttctggcgc atggtaggga ctggtcagac agaagggcgg gacatcagga tggtggcgac
    13621 gggaaaactg gacgagcctg cggcagcagc gctcagacga acggagccgg agcaggcggc
    13681 agatcgaccg atgatcgggg cgcgggtgct ctgtcacgcg ctccagcgcg aacgggttga
    13741 cacaatcttc ggctatccgg gcggcgtcgt gatcccgctc tacgatgtcc tgccggagtt
    13801 cgacattcat catgtcctcg ttcgccatga gcagggcgct gggcacgccg ccgatggcta
    13861 cgcccgcgcg accggcaagg tcggcgtctg cctggcgaca tccgggcccg gcgcgacgaa
    13921 cctggtgacg ccaatcgcga ccgcgatgct cgactcggtt ccactcgtcg ccatcaccgg
    13981 ccaagtgtcg cagggcgtca tcggcagcga cgcctttcag gagatcgata tcaccggcat
    14041 cacgctgccg atcacgaagc acaacttcct cgtgcgcagt gtccacgaca ttggtccgac
    14101 gatccagaag gcatttcacc tcgccagcag cggccgcccg ggtcccgtcc tcgtcgatat
    14161 ccccaaagat gtcctgctgg ccaccggcga gcttggcgag ccggccaagc ttgaactgca
    14221 gggctacaag ccgacctaca cgccgcaccg tcgccagatc cggctggcag ctcaggagat
    14281 ccgcaacgcc cgtaagccgc tgatcctggc cggtcacggc atcctgatct ccggcgcgga
    14341 aaatgagctg aagacgctct cagagcgagc gcagatcccg gttgggctga cgttgctggg
    14401 tatcggcggc ttcccgcaat cgcacccgct ctgcctcggc atgatcggca tgcacggctt
    14461 cgcgcacgcc aaccgcgcca tccatgaggc cgacctgatc atcggcgtcg gcatgcgctt
    14521 cgacgaccgg gtgaccggcc ggctgagcga gttcgctccc aacgcccgtg tcgttcacat
    14581 cgacatcgac ccggccgaga tcggcaagaa tgtgcctgcc gacgtgccgg tcgtcggtga
    14641 cgccaggcag gcgctgggcc tgctggctgc tgagctggaa gatcagcagc acgaggcgtg
    14701 gcttggcgag atcgccgaat ggcgcagaat cccgccctac cgctccagcg ccgaatcgca
    14761 gacgttgctg ccgcagttcg tcatcgagcg gctgcacgac ctgaccgagg gcaaggccgt
    14821 cgtgacgacc gacgtcggtc agcaccagat gtgggccgcc cagttctaca agtgcgatca
    14881 gccgaaccag tggatctcct
//
LOCUS       DASUGJ010000008         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_278902, whole genome shotgun sequence.
ACCESSION   DASUGJ010000008 DASUGJ010000000
VERSION     DASUGJ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278902"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..806)
                     /locus_tag="VFV93_00265"
     CDS             complement(81..806)
                     /locus_tag="VFV93_00265"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HEX5163796.1"
                     /translation="MNLGKLKLAAPASLLAAALLAGACADTGETQNVSENVDLDGATS
                     AVVLVRMGAVDLDLAGGARGMLDADFSYNVEEWEPDVNWKVTDGVGRLEIDQSGSGSV
                     NIFDLDEIENDWDLHLNDEIQTTLDVELGAGDSTLKVGSLALTDLDVDTGAGDTTIDL
                     AGDWHNDLDATVEAGAGQVTLRVPADVGVRIETDTGIVDVDHSGLNKDGDVYTNDAYG
                     VSDITLTINIDAGVGKVDLEVVE"
     gene            1098..2639
                     /locus_tag="VFV93_00270"
     CDS             1098..2639
                     /locus_tag="VFV93_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5163797.1"
                     /translation="MGDDENQRQVSAIRPEDLGLGILGSVLDVALDGIIVVGEGRRIV
                     YANPAVGEIAGCAHQTMIGRDLLDFIAPAAREAMRGYSESSLAGHTGRRRSTVLRPDG
                     NEREIEYSNMLLSGPDGPLICAIVRDVTDVRRREREASSLAQIAASLTLDQPMEQTLD
                     SLAAHVVDATRAIATAVLLMDESGKVLRMVGTNGLPEGAGAALEAAWPAAVNRSPVAR
                     AFQTQRVFIMRDARSGERETPEYVPVNTVAWDTIVAVPLIYHGRGLGVLNAYFLPDAH
                     PDGPEVEFLKAIADQAAVAVENARLYHAAQQKAALEERQRLARELHDSVSQALYGIGL
                     GARTARTLLDQSPEQAGEPLDYVLQLAEAGLTEMRSLIFELRPESLEREGIVSALNRQ
                     AKALRARHGLHIEVTTCDEPEARLDIKEAIYRIAQEALHNVVKHAHATSVAIRLDDGP
                     TGLTLTIHDNGVGFDPSGSFPGHLGLSSMRERATRLGGAFTIQSARTRGTTLTATVPV
                     ASAES"
BASE COUNT          472 a          947 c          815 g          474 t
ORIGIN      
        1 tacgagctca agcgcccgcc gattgattga cgccatcccg tcgtccgccg ccctcagggc
       61 ggtggacgac gggatggctg ctattccacg acctccagat cgaccttgcc gacgccggca
      121 tcgatgttga ttgtcagcgt gatgtcgctg acaccgtatg cgtcgttcgt gtagacgtcc
      181 ccatccttgt tcaggccgga gtgatcgaca tcgacaatgc cggtgtccgt ctcgattcgg
      241 acgccaacgt ctgccgggac tcgcaacgtc acctgacccg cgccggcctc gaccgtcgca
      301 tccagatcgt tgtgccagtc gccggccagg tcgatcgtcg tgtcaccagc cccggtgtcg
      361 acatccagat ccgtcagcgc cagtgagccg accttcagcg tgctgtcacc ggcgccaagc
      421 tcgacatcca gcgtcgtctg aatctcgtcg ttcaggtgca gatcccagtc attctcgatc
      481 tcgtcgagat cgaagatatt cacgctgccc gacccagact ggtcgatttc gagccggccg
      541 acaccgtcgg tgaccttcca attgacatcc ggttcccact cctcgacgtt gtaggagaag
      601 tcggcgtcga gcatgccgcg agcccctccg gccagatcaa ggtcgacagc gcccatccgt
      661 accagcacca cggcgctggt cgcaccatca agatcgacat tctccgatac gttctgtgtc
      721 tcgccggtat cagcgcaggc ccctgccagc aacgcggctg ccagcaggct cgccggcgcc
      781 gccagcttca gttttcccag gttcatcgtc gaacccgatc ctctctcagg acagcatcgt
      841 ccagttcggt acacgcgcac gccgaacggc tcggcatccg cgatgcacat cggttcagtt
      901 gtacgttggc tggctgcgac ccgccacgag tggatggaca gtcccagacc agacctgcgt
      961 acgatcgttg gaatccgacc tttggccgat gactacaccg gcaggtctgt ggattgattg
     1021 gatcgtggag tccacccggg tggactgcgg caggcaacta cactggttgg gtggaatcgt
     1081 tcggactggc acgcgagatg ggcgacgacg aaaatcagcg acaggtcagc gcgatccggc
     1141 cggaggatct cggcctcggc attctcggca gtgtcctcga tgtcgcgctc gacgggatta
     1201 tcgttgtcgg agagggacgc cggatcgtgt atgccaatcc ggccgtcgga gagattgccg
     1261 gttgcgcgca tcagacgatg atcggtcgtg acctcctcga tttcatcgcc ccagccgcgc
     1321 gggaagccat gcgcggctac agtgagtcca gcctcgccgg gcataccggc cggcggcgat
     1381 caaccgtcct tcgtcctgac ggcaacgagc gcgagatcga atactccaac atgctgctgt
     1441 ctggtcccga tgggccgctg atctgcgcca tcgtccgcga tgtgaccgac gttcggcggc
     1501 gcgagcgcga ggccagctca ctggcccaga tcgccgcctc gctgacactc gaccagccaa
     1561 tggagcaaac gctcgacagt ctcgccgccc acgtcgtcga cgccacccgc gcgatcgcga
     1621 ccgccgtcct gttgatggac gagagcggca aggtgttgcg gatggttggc accaacggcc
     1681 tgccggaggg cgccggcgcg gccctcgaag ccgcctggcc ggccgccgtg aatcgatccc
     1741 cggtcgcccg cgccttccag actcagcgcg tcttcatcat gcgcgacgcc cgcagtggcg
     1801 agcgtgagac gcctgagtac gtgcccgtca acactgtcgc ctgggataca atcgtcgccg
     1861 taccgctcat ctaccacggc cgtggacttg gggtcctcaa cgcctacttc ctgcccgatg
     1921 cccacccgga tggccccgaa gtcgagtttc tgaaggcgat cgccgaccag gcagccgtcg
     1981 ccgttgagaa cgcccgtctc tatcacgctg cccagcagaa agccgccctc gaggaacgcc
     2041 agcggctggc gcgcgagttg cacgactccg tctcgcaggc cctctacggc atcggcctcg
     2101 gtgcccgaac ggctcgaacg ctgctcgatc agtcgcccga acaggccggt gaaccgctgg
     2161 attatgtcct gcaactcgcg gaagcgggcc tgaccgagat gcgctcgctg atcttcgagc
     2221 tacgcccgga atcgctggaa cgcgagggga tcgtcagcgc actcaaccgg caggcgaagg
     2281 cgctacgcgc ccgccacggc ctgcatatcg aggtcacgac ctgcgacgaa cccgaagccc
     2341 ggctcgacat caaggaagcc atctaccgga tcgcccagga ggcgctgcac aacgtcgtca
     2401 agcacgccca cgccaccagc gtcgctatcc gcctcgacga cggcccgacc ggtctcacgc
     2461 tgacgattca cgacaacggc gtcggcttcg acccctccgg ctcgttccca ggccacctcg
     2521 gtctgagctc catgcgcgag cgcgccaccc gcctcggcgg cgccttcacc atccaaagcg
     2581 cccgcacccg cggcacaacc ctgacggcga cggtgccggt ggcgagtgct gagtcctgag
     2641 tgctgcgtgc tgagttgata gcgttgcgtt acaggtaacc cgggcggacg gccttcacga
     2701 ggattcgc
//
LOCUS       DASUGJ010000009        10793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_418107, whole genome shotgun sequence.
ACCESSION   DASUGJ010000009 DASUGJ010000000
VERSION     DASUGJ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418107"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFV93_00275"
     CDS             complement(<1..528)
                     /locus_tag="VFV93_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163798.1"
                     /translation="MTSRNLAEVIDDLAEDARAGRLSRRGVLKRAMALGLSAPVVAGL
                     LAACGGDDDDDDAAPANTAASGGSEPTSTTAADAEPTDTTASGSEGSATAEGSAEATE
                     APSEPTASEASAPPAQAGGGGLLRILQWQAPTILNPHLSTGYKDYDCSRIAYQPLADF
                     NLAGDGQPFLAAEFPT"
     gene            923..1399
                     /locus_tag="VFV93_00280"
     CDS             923..1399
                     /locus_tag="VFV93_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5163799.1"
                     /translation="MAALDATDLQLIRLLQDQPRASHAELSRLTGISISTVRRRVTRL
                     LDSGAISFSVYPNVWEMGYKAVSLIGINADYSRIEEIANQIREIEEVTLVLITLGRYD
                     LFAGVALHDVEEVYPFLKERIATIEGVKDFETFVSGAVIKEFREWRVPQEDLTGDR"
     gene            1360..1647
                     /locus_tag="VFV93_00285"
     CDS             1360..1647
                     /locus_tag="VFV93_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163800.1"
                     /translation="MARATGGPDWRPLIGFDHFQLNLELLAELVDLSAPETIATATSK
                     AAEAIGSSDFDVLAPGKQADVLAVRGNAVDDIRAPQRPAGAQVRSGHGRPR"
     gene            complement(1644..2873)
                     /locus_tag="VFV93_00290"
     CDS             complement(1644..2873)
                     /locus_tag="VFV93_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX5163801.1"
                     /translation="MQDERSRARVKGMQPASRAVHSGERPEQPQFTPVVTPIYPGSAY
                     VYDDLEIMDAALGGAEGKYVYTRYGNPTTTALETAIADMEGTTSAVSFSSGMAAVHAA
                     IMTSVAPGDAIVASQDLYGATYQMLSTHMREWGCTVEFVDTLDLAAVADAVSRLSPAL
                     IVCEVISNPLMRVTDVPRIVEIAKTVRAAVLVDATFNSPVLYTPAADGAQLIVHSLTK
                     YIAGHGDVTGGIVATTSMRRERLANFAKMAGAILGPFEAWLALRGLKTLPLRMERQSR
                     NAAIVAERLASHPRVANVNYPGLPGHASHSTAKRLFGERGYGGMLSIDIRDAGREQAF
                     AFLEALKMVVPATSLGDVYSLSLYPAMSSHRALTPEERAAIGIGEGLLRISIGIEDPE
                     DILADLDQALERAARIS"
     gene            3096..3329
                     /locus_tag="VFV93_00295"
     CDS             3096..3329
                     /locus_tag="VFV93_00295"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX5163802.1"
                     /translation="MTVRNQGNVAAGHSYVRFNEWPISVAGLAPGAIRTMRFYRKECE
                     VNSTISVDASNEVSEEVSERNESNNRMPRLVLC"
     gene            complement(3532..4056)
                     /locus_tag="VFV93_00300"
     CDS             complement(3532..4056)
                     /locus_tag="VFV93_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5163803.1"
                     /translation="MDGIAVLKSAYAGAHMWYRGTIEGLTSEDANRVPPGIAHPIGQQ
                     IAHILHAEDGMLNMVLRGQPMLWERDGWGARLDLPLQLAQTSDSARAFRCDAQQLAQL
                     GEYADAVFANTDAYLDSLTPDDLDAEADLTAAGMGTMPLGVFLTQFLLGNTYGHTGEI
                     STLKGMLGRTGYPF"
     regulatory      4429..4513
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            4572..5420
                     /locus_tag="VFV93_00305"
     CDS             4572..5420
                     /locus_tag="VFV93_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5163804.1"
                     /translation="MGQGQQRIVIVDDDPVGSRILRYILAEEGYDAITVTRGSQVFEH
                     VIGQDTSLVLLDINLPDVDGFALCRELRARRYNGPLMFLTSRSSITDRLEGFRLGADD
                     YVTKPYDHLELVARIQAIIRRSNRADAQALGTVVHVEDAVLSLGELTYASKVVPPTIL
                     TPTEMRILECLMRNSRIVISRETLIERVWGFDFYGDTNRVDVYIRRVRRKIEVDPTMP
                     EYLHTVRGIGYVFRVEPRCLVQDSNGIVNDEPAASWMTHSDEISGYAAGVLLSEAAGL
                     ALAASQ"
     gene            6106..7101
                     /locus_tag="VFV93_00310"
     CDS             6106..7101
                     /locus_tag="VFV93_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX5163805.1"
                     /translation="MLVTSQPTASDRYLGCLLGLAIGDALGMPAADRDGEQPISNYEP
                     LLDPDGDQLVPAGQFTSHTELALCLVESLISANGFIDPDTAGYRFVQVLHNEHSHFLD
                     ATTRAALEQADATGDYQNGLAGDDPIEPGPAARIAPIGLVHALGQINTEMFVREVMRA
                     TLITHAAPEAVNAALAMAWAVGLAVQHDVVPEMVASEVLAFIDEDETAVKVRIAAEAL
                     VSRRSAARDADILARIGPGETIAEAVARALYLFGIHADDFPAAVLAAVNSGPQGAAIG
                     AMTGALVGAHAGAKALPVPLVEGLDGRMYILMAAPALFRTAQRRAGLFLPLHQRP"
     gene            7098..7775
                     /locus_tag="VFV93_00315"
     CDS             7098..7775
                     /locus_tag="VFV93_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX5163806.1"
                     /translation="MSAKRIQAVVFDLDGLLIDSEPLQAWAWHEYVARFGAVMTPNVL
                     YRMLGLRAVDAVKIPIEMLDLPVDAATALRERDEIFLAAVPGAIQAHAGAHDLIDALK
                     SRNIPLALATSGHRRYVDLALASAGLAGLFDQEVTAELVTRGKPNPDVYLRAAELLNT
                     APNACLALEDAPNGVTAAKAAGMYCFAIPGDHATPASVAHADATLASLGDVMGEMQRR
                     GWVFRDA"
     gene            complement(8094..8582)
                     /locus_tag="VFV93_00320"
     CDS             complement(8094..8582)
                     /locus_tag="VFV93_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX5163807.1"
                     /translation="MADTYPARVEQPLKRILGWDDIMREVDDLVARIGTDYDAMLVIT
                     RGGMVPACLISERLDLRNILVAAVQLYTGIGTTLDDPVFLQFPSDTLLHGKRILVVDD
                     VWDSGRTAMSVRQRIQTAGGIPTLAVLHYKPRASRFPEQQPDFYAEMTDDWLVYPWDD
                     ER"
     gene            8916..10037
                     /gene="dnaJ"
                     /locus_tag="VFV93_00325"
     CDS             8916..10037
                     /gene="dnaJ"
                     /locus_tag="VFV93_00325"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX5163808.1"
                     /translation="MADKRDYYEVLGLSRNASNDDIRKAYRRLARQYHPDINKDDGAE
                     DKFKEINEAHEVLSDADRRAAYDRFGHAAVNGGAGGDPFGFGGRSPFGDLFETFFSGG
                     ATTRQRQRTVQRGGDIRISLDLSFEEAVFGVEREVDVNRYEPCDDCHGTRMRDGQKPP
                     TCPQCNGSGEVRRVQNTILGQFMTATTCDRCGGEGVTISDPCPTCRGRGRVSKTRKIV
                     VSVPPGVDENSTLRLTGQGEQIRDGVPGNLFVALKIKQHKELGRTGKHILYDLPVTVA
                     QAVLGDQIEIPTVDGLSTFELPAGTQPNQQFRMRGKGVPDLRGGDRGDQIVTVHVVIP
                     TQLSPEQRQLFEQLDGTLETATMPKEKGFFSKMKDALGV"
     gene            complement(10168..10692)
                     /locus_tag="VFV93_00330"
     CDS             complement(10168..10692)
                     /locus_tag="VFV93_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163809.1"
                     /translation="MQSLVALTRTLLPQSWLHGMLTHLISLIMLSAAITVLWKFNVNL
                     KRRVAADQHLLVMYAATVVAIGLFSPHFFQYDCVILVLPMLIALELIPLTRQFRLALA
                     AVLILGWATPLRYGLFEHRPWPIPLLNAPWAVLFTAFLLLTLHRYVAGARSAASEQPS
                     RQVQPAPLGATPAS"
BASE COUNT         1911 a         3640 c         3410 g         1832 t
ORIGIN      
        1 ggttgggaac tcggctgcca gaaatggctg accgtcgccg gccagattga agtcggccag
       61 cggctggtag gcgatccgcg agcagtcata gtccttgtag ccggtcgaca ggtgcgggtt
      121 gaggatggtt ggcgcctgcc actggaggat ccgcagtagc ccgccaccgc cggcctgggc
      181 gggcggcgcg gacgcctcgg acgcggtcgg ttcggacggg gcttcggtcg cttcggcaga
      241 gccctcggcc gtcgccgatc cctcgctccc ggacgcggtc gtgtcggtcg gctcggcgtc
      301 ggctgccgtc gtgctggtcg gttcgctgcc gcccgacgca gccgtgttcg ctggcgcggc
      361 gtcgtcatcg tcgtcgtcgc cgccgcaggc cgccagcagc ccggcgacga ccggcgcgct
      421 gagccccagt gccatcgccc gcttcagcac gccgcgccgg ctgagcctcc cggcgcgggc
      481 gtcttcggcc agatcatcaa tcacttccgc caggttccgt gatgtcatcc cactcactcc
      541 tgcatgtatc ccaaccgcgc gccgttgacc acgacgcgcc ggttcgactc gcttccgcct
      601 ccaggcgttc ggatgagcca tcgctcgctt gtcccgccac ctcgctctcc tcttgccccg
      661 cgtacgagcc gtcacggggt acggccaact gccccaccag ccacgtccca gcaaggggag
      721 tagaccgatg tggcccgttg cgctcgtcgc actgccggtg tgcgtctggg aaccgaatgc
      781 agcgtcgcgg tttcgttcac aggcgagttc ggcggatgct agcgccgata ttcgccactg
      841 tcaacaccgg atgtgattga atcgtccaca cttcaaccac acgtgcgtag aatcgttcgc
      901 aatcgatcgg aatgatgaac agatggcggc actggacgcg accgaccttc agttgatccg
      961 gttgttgcag gatcagccgc gcgcctcgca cgccgagctg tcgcgactga ctggcatcag
     1021 catctcgacc gtcaggcggc gggtgacgag attgctcgac tccggcgcga tctcattctc
     1081 ggtctatccc aacgtctggg agatgggtta caaggccgtc agcctgatcg gcatcaacgc
     1141 cgattacagc cggatcgagg agatcgcgaa tcagatccgc gagatcgagg aggtgacgct
     1201 ggtgctgatt accctcggcc gctacgacct gttcgccggc gtcgcgctgc acgatgttga
     1261 ggaggtctac ccgttcctga aggagcgcat cgcaacgatc gagggcgtca aggacttcga
     1321 gacgttcgtc tccggcgcgg tcatcaagga gttcagggag tggcgcgtgc cacaggagga
     1381 cctgactggc gaccgttgat cggcttcgat cacttccagc tcaacctgga actgctggcc
     1441 gagctggtcg acctctcggc gccggagacg atcgcgacgg cgacttcgaa ggctgccgag
     1501 gcgatcggct cgtccgactt cgatgtcctc gcgccgggca agcaggccga cgtgttggcc
     1561 gtgcgcggca acgccgtcga tgacatccgc gctccgcaac gtccagctgg tgctcaagtc
     1621 cggtcaggtc atggtcgacc gcgctagctg atgcgcgccg ctcgctccag cgcctgatcg
     1681 aggtcggcca ggatgtcctc ggggtcctca ataccgatcg agatgcgcag gagcccctca
     1741 ccgataccga tcgccgcgcg ctcctccggg gtcagcgcgc ggtgcgagga catcgccggg
     1801 tagagcgaga gcgagtagac gtctcccaac gacgtggccg ggacgaccat cttgagcgcc
     1861 tccaggaagg caaaggcctg ctcccggccg gcgtcgcgga tgtcgatcga gagcatcccg
     1921 ccgtagccgc gctcgccgaa cagccgtttg gccgtcgagt ggctggcgtg gcccggcagg
     1981 ccagggtagt tgacgttggc aacccgcggg tggctcgcca ggcgttcggc gacgatcgcc
     2041 gcgttgcgtg actgccgctc catgcgcaat ggcagcgtct tgaggccgcg caacgccagc
     2101 caggcctcaa acgggccgag gatcgcgccg gccatcttgg cgaagttagc cagccgctcg
     2161 cgccgcatcg aggtcgtcgc gacgatgccg ccggtcacgt cgccatgccc agcgatgtac
     2221 ttcgtcagcg agtggacgat cagctgtgcg ccatcggcag ccggcgtgta gagcaccggc
     2281 gagttgaagg tcgcgtcaac cagaacggcc gcacggaccg tcttcgcgat ctcaacgatc
     2341 ctcggcacgt cggtcacccg catcagcggg ttggagatca cctcgcagac gatcaacgcc
     2401 ggcgagaggc gactgacggc atcggcgaca gccgccagat cgagcgtgtc aacgaactcg
     2461 accgtgcagc cccactcgcg catgtgggtt gagagcatct gataggtcgc gccgtacaga
     2521 tcctgcgagg cgacgatggc atcgcccggc gcgacactag tcatgatcgc agcatgaaca
     2581 gccgccatcc cggatgagaa cgagacggcc gaggtcgtcc cttccatgtc ggcgatggcc
     2641 gtctccaacg ccgtcgtcgt cgggttgccg tagcgggtgt agacgtactt gccctcggcc
     2701 ccgcccagcg cagcgtccat gatctccaga tcgtcgtaga cgtaggccga gcctggatag
     2761 atcggtgtca cgaccggcgt gaactgcggc tgctccggcc gttcgccgct atggacggcc
     2821 cggctggccg gctgcatccc ctttacccgc gccctgctgc gctcatcctg cacggtatcc
     2881 ctgcctcccc atcgatcgca tgtcactcac cgccatagta atggcgcgcg gtcgatgtcg
     2941 ccagcacccc cgcgacgcag ctggctctat ccaggacaac agcggtcgtg tcggatgcta
     3001 tgctgcccaa ccggaccgca tcggctgtac cagcgacatg gtcgctgtgg accagcgtcg
     3061 tagcggggtg cggccgcgcc ggctggtaca catccgtgac ggttcgcaat cagggcaacg
     3121 tggcagccgg ccactcttac gtccgcttca acgagtggcc catctccgtc gcggggctcg
     3181 ctccgggagc gatcaggacc atgcgcttct accggaagga gtgcgaggtc aatagcacca
     3241 tctcggtcga tgcgtccaac gaggtcagcg aagaggtcag cgaacgcaac gagtccaaca
     3301 accggatgcc ccggttggtc ctctgctaat ggctcgcgct tcacccatgc tgcggatcgg
     3361 ctcgccactg cgtgacgtag cagaggtggg cgtctgccag ccagagtgta tgcagacgtg
     3421 ggcgagtcgt ggccagggct ttgaggccct ttgcgccatg ctcagggcct cacctcaagt
     3481 tgtgcaggag attcccgcac gcagcggcgt gtgcctgtac cgagctggag tctagaaggg
     3541 ataccctgtg cgaccgagca tgcccttgag cgtagagatt tcgccggtgt ggccgtaggt
     3601 atttcccaac aggaattgag tcaggaagac gccaagcggc atcgtaccca tcccggcagc
     3661 ggttagatcg gcctcggcgt cgaggtcgtc cggcgtcagg ctgtccagat aggcatcagt
     3721 gttggcaaaa acggcgtcgg cgtactcgcc caactgggcc agttgctggg catcgcagcg
     3781 gaacgcacgt gccgagtcgg atgtctgcgc caactgcagc ggcagatcca gcctggcacc
     3841 ccagccgtct cgttcccaga gcatcggttg accgcgaagc accatgttca acatgccgtc
     3901 ctcagcatgc agaatatggg cgatctgttg cccgattgga tgggcgatgc ccggcggaac
     3961 gcggttggcg tcctcgctcg tgagcccctc gatggtgcct cgataccaca tgtgcgcgcc
     4021 ggcatacgcg cttttcagca ccgcaatccc gtccactgca ctgccctcca gacgtcatca
     4081 catcgttggc tacccacgtt ggtgcaacca gcggtaatac gccgccgctg ctcacatgga
     4141 tcacgacgcg gatggagcat cggcaatgtc gcccaacctg tgacactcct gagcctccgt
     4201 gttggcgtga gatcgcgaac gggcatgctc tggtcgtcac gtcacatccg gcgtgcgggg
     4261 aatcgccgga cgtcccgtat gccctgagag atgattcgtc aggccatgcc cgcgctccac
     4321 ctctgtcgct cgatcgaagc catttgcccc ttccgatgga aggcgaatcg tcctgccggc
     4381 aatgaggacc ttcggccttg gctggggcat catccacccg atacgatgca caccactgga
     4441 agcgatgacg cgcgggctgt aatctggacg cctcgcccgc gtcgagcaac tggcgtaacc
     4501 ggccgatgac catcgagccg gtttttgtgt gcctgccggc ggcaactgtg gtgcatggaa
     4561 aggttcgccg gatgggacag ggacagcagc ggatcgtcat cgtggatgat gatccagttg
     4621 gatcacggat actccgctac atcctcgccg aggaaggcta cgacgccatc accgtcaccc
     4681 gtggcagcca ggtgttcgaa catgtcatcg gccaggacac gagcctcgtc ctgctcgaca
     4741 tcaatctgcc ggatgtcgat ggcttcgcgc tctgccggga gttacgcgcg cgccgttaca
     4801 acggcccgct catgttcctg accagccgct ccagcatcac cgaccgactg gaagggtttc
     4861 gactgggcgc tgacgactac gtgaccaagc cctacgacca cctggaactg gtggcccgca
     4921 tccaggccat catccgacgg tcgaaccggg ccgacgctca ggcattgggg acggttgtcc
     4981 atgtcgagga cgctgtactc tcgctcggcg agctcaccta cgccagcaag gtcgtcccgc
     5041 caacgattct gacgccaacc gagatgcgca ttctggaatg tctgatgcgc aacagccgga
     5101 tcgtgatcag ccgcgagacg ttgatcgagc gggtctgggg cttcgacttc tatggcgaca
     5161 cgaaccgcgt cgacgtctac attcgccgcg tccggcgcaa gatcgaagtc gatccgacca
     5221 tgccggaata ccttcatacg gtgcgcggca tcggctacgt cttccgcgtg gaaccgcggt
     5281 gtctggtgca agacagcaat gggatcgtca acgacgaacc cgctgcctcc tggatgacgc
     5341 acagcgacga aatatctggc tacgcagctg gagtgctgct cagcgaggct gccgggctgg
     5401 cactcgctgc cagccagtaa cgccagacgg ggcaacgcgt gacctcgaac cgagatcagg
     5461 attgatggcg tcatcgtgat ccttcaggcc gtcccagcag gtacttcctg ctgggacgga
     5521 caatgacgat tggtctattc aagccgtgac gcgactccgc tacgctgatc taactgtcag
     5581 ccggagatca cacggatgga tggaccgcca tggcgatggc cgcgtctcgc gttacggatc
     5641 gacccactgc ccgcgttgcg accgccacag ttgatgagtc cgttacgtgc gggccgctgg
     5701 tgtatcgagg gattgtcttc gccctcctgt ttgaagcgag cgctggcgtc ggactctggg
     5761 ccatctacca tctggtcacc tccctcttct aacccacgct gtgccggaac ccgaatcaga
     5821 acaccactcc tgcgccacac caccatcagc ctgctccaac gggtcggcat gcggctggtc
     5881 gcagcgtctc tccggcgcta cgagccgtgt cccgctgaag ccctcgaacc ggtgctgctc
     5941 ccaacgttga ctcgcgccaa cgccctgtgt gtcagcgcga ggctccctca tgatcattct
     6001 tcaactcacg cttcagcatg accagcgaga cgaattactc ggctatccgc aatgcggtac
     6061 gcttttcgtg agattgtgat tcgcctcacg atggacaggc tgccgatgct cgtgacctcg
     6121 caaccgaccg ccagcgaccg ctacctgggc tgcctgcttg ggctcgctat cggcgacgcg
     6181 ctggggatgc cggccgccga ccgggacggt gagcagccaa tcagcaacta cgagcccctg
     6241 ctggaccccg acggtgacca gctggtgccg gctgggcagt tcacctcgca cactgagctg
     6301 gcgctctgcc tcgtcgaatc gctcatctcc gccaacgggt tcatcgaccc cgacacagcc
     6361 ggttaccgct tcgtccaggt gctgcacaac gagcacagcc acttcctcga cgcgacaacg
     6421 cgggccgccc tcgaacaggc cgacgcgacc ggcgactacc agaacgggct ggccggggat
     6481 gaccccatcg agccgggacc ggcggcacgg atcgcgccga tcgggctggt ccacgcgctg
     6541 ggccagatca acaccgagat gttcgttcgc gaggttatgc gggcgacgct gattacccac
     6601 gccgcaccgg aggctgtcaa cgccgcgctg gcgatggcct gggccgtcgg cctggccgtt
     6661 cagcatgacg ttgtcccgga gatggtcgcc agcgaggtgc tggccttcat cgacgaggac
     6721 gagacggccg tgaaggtccg catcgccgcc gaggcgctcg tcagccgcag atcggctgcc
     6781 cgggacgcgg acatcctggc gaggatcggc cccggagaga cgatcgccga ggcggtcgct
     6841 cgcgcgctct acctgttcgg catccatgca gacgacttcc cagccgcggt tctggcggct
     6901 gtcaacagcg gcccgcaagg cgcggcgatt ggcgcaatga cgggtgcgct ggttggcgcg
     6961 cacgctggcg cgaaggcgct gccggtgcca ctcgtcgagg ggctcgatgg ccggatgtac
     7021 atcctgatgg cagcgccagc cctgttccgg accgcgcaac gccgggcggg gctgttcctc
     7081 ccgctccacc agcggccatg agcgcgaagc ggattcaggc cgtcgtcttc gacctcgacg
     7141 gactgctgat cgactccgag ccgttgcagg cctgggcgtg gcacgaatac gtcgcccgtt
     7201 ttggcgccgt catgacaccg aacgtcctct accggatgct cggcctgcgg gccgtcgacg
     7261 ccgttaagat tccgatcgag atgctggacc tgccggtcga cgcggcgacc gccctgcgcg
     7321 agcgcgacga gatcttcctg gcagccgtcc ccggcgcgat ccaggcccac gctggcgccc
     7381 acgacctgat cgatgccctc aagtcccgca acatcccgct cgcgctggcg acctccggcc
     7441 accgccgcta cgttgacctc gccctggcca gcgccgggct ggccggcctc ttcgaccagg
     7501 aagtcacggc agaactggtc acgcgcggca agcccaaccc ggatgtctac ctgcgcgccg
     7561 ccgaactact gaacaccgca ccgaacgcct gcctggccct cgaagacgcc cccaacggcg
     7621 tcaccgccgc caaggccgcc ggcatgtact gcttcgccat ccccggcgac cacgccactc
     7681 ccgccagcgt cgcccacgcc gacgccacgc tggcgtcctt gggagatgtg atgggtgaga
     7741 tgcaacggcg ggggtgggtt tttcgggacg cgtgacgcgg gacgcgggac gcgtgaacca
     7801 gacggacggc gctggtgagg acgcggccct cttaccctag tcatccaccg cccagagggc
     7861 acccggcagc gaagatcgcc tggcgtgaag ggggcgactg gccgcccagg atgcgagcgt
     7921 ttggctgccg aatcacccac tcgtacaact gacgccgagc gatccttcgc tgcggctcag
     7981 gggctgctcg tactgtcctc accgacgcgg ttcatctggg gcagtagccg cgtcccgcgt
     8041 tccgcgtccc acgtcccacg tcccacgtcc cacgtcccgc gtcccgaaac aatctaccgc
     8101 tcgtcatccc acgggtagac cagccagtcg tcggtcatct cggcgtagaa atcgggctgt
     8161 tgctcgggga agcgtgaggc gcgcggcttg tagtggagga cggccagcgt tgggatgccg
     8221 ccggcggtct ggatccgctg gcggacactc atggccgttc ggccgctgtc ccagacatca
     8281 tcgacgacca ggatgcgctt gccgtgcagc agcgtgtcgc tggggaactg caggaagacc
     8341 ggatcatcca gcgtcgtgcc gatacccgtg tatagctgga cggcagcaac gaggatgttg
     8401 cgcaggtcga gccgctcgga gatcaggcag gccggcacca tcccgccgcg cgtgatgacc
     8461 agcatcgcgt cgtagtctgt cccaatccgc gcgacgagat catcgacctc gcgcatgatg
     8521 tcgtcccagc cgagaatgcg cttcagcggc tgctcgacgc gggccggata cgtgtctgcc
     8581 atcccagatt cccacctttg tatgcaatca tcaaacatcc acgacctgcc gtggattgct
     8641 cagcgtagag tgacgcgatc ggtgcgctgc accaccacaa tgctacccga ttcgggcatg
     8701 cgagccgcag ccggctgtcg tgcgcgcaag gaaaccggcc ggaactggca ctctcgtccc
     8761 gaaagtgcta agacgcgttg acggttgccg cctcgctgtt atcatttgag atactgccac
     8821 ggtctgaacc gtgaccacga agcgatgttg acaacgccgg tccccgcccc gcaggcacaa
     8881 atctgaccgg ctgccctgaa tcaggtgagc aatagatggc cgacaagcgc gattactacg
     8941 aggtgctcgg actcagccgc aacgccagca acgacgacat ccgcaaggcc taccggcggc
     9001 tggcccgtca gtaccacccc gacatcaaca aggacgacgg ggccgaggac aagttcaagg
     9061 agatcaacga agcccacgag gtgctctccg acgccgatcg gcgcgcggcc tacgatcgct
     9121 tcggtcacgc ggccgtcaac ggcggcgccg gcggcgaccc gttcggtttc ggcgggcgct
     9181 ctcccttcgg tgacctgttc gagacgttct tcagcggcgg cgcgacgacc cgccagcggc
     9241 agcgcaccgt ccagcgcggc ggcgacatcc gcatctcgct cgacctcagc ttcgaggagg
     9301 ccgtcttcgg cgtcgagcgc gaggtcgacg tcaaccgcta cgaaccctgc gacgactgcc
     9361 acggtacgcg gatgcgcgac ggccagaaac cgccaacctg cccgcagtgc aacggcagcg
     9421 gcgaggtgcg ccgggtccag aacacgattc tcggccagtt catgacggcg acgacctgcg
     9481 accgctgcgg cggcgagggg gtcacgatca gcgatccctg cccgacctgc cggggccgtg
     9541 gccgggtcag caagacccgc aagatcgtcg tctcggtccc gccgggcgtc gatgagaact
     9601 caacgctgcg gctgaccggc cagggcgagc agatccgcga cggcgtgccg ggcaacctgt
     9661 tcgtcgccct caagatcaag cagcacaaag agctgggccg gaccggcaag cacatcctct
     9721 acgacctgcc ggtcaccgtc gcccaggccg tcctcggcga ccagatcgag atcccgaccg
     9781 tcgatggcct ctcaaccttc gagctgccgg ccggcaccca gccgaaccag cagttccgga
     9841 tgcgcggcaa gggcgtcccg gatctgcgcg gaggcgaccg cggcgaccag atcgtcaccg
     9901 tccacgtcgt catcccgacc cagctctcgc ccgagcaacg ccagctcttc gagcagctcg
     9961 acggcacgct cgagacggca accatgccca aggagaaggg cttcttctcg aagatgaagg
    10021 acgcgctcgg cgtctgacgt tcgtgagtcc ggcgcgcgtt accagcgaag ccgcggaact
    10081 cgaagcaaca gaagatcggt tgaatcagga gttcgcttgt gaggcggtag ccggccaccc
    10141 ggctcgtctc gtcctgctgg atcgacatca actcgcgggc gtcgcaccaa gtggggctgg
    10201 ctggacctgc cgcgatggct gttcgcttgc cgccgaacga gcgcctgcaa cgtaccgatg
    10261 gagcgtcagc aagagaaacg ccgtgaatag aaccgcccat ggtgcattga gcagcgggat
    10321 cggccacgga cgatgctcga acaatccgta gcgaagcggc gtcgcccatc ccaggatgag
    10381 gaccgcagcg agcgcaagcc ggaattgtcg tgtcaatggg atgagttcca gagcgatcag
    10441 catgggaaga acaagtatga cgcagtcgta ctggaagaaa tgcgggctga acagtccgat
    10501 cgcaaccacc gtcgcggcgt acatcacgag tagatgctga tctgccgcca cacgccgctt
    10561 caggttcacg ttgaacttcc acaacacggt gatcgctgcg ctgagcatga tcaggctgat
    10621 gaggtgggtc aacatcccat gcagccagct ctgcggaagc agtgtccgcg tcaacgccac
    10681 gagcgactgc atcttccaat ccatgttggc cgcgacggca accacgtagg cgtccgatcg
    10741 caggaggcca atgtaccctc gcacgccttc taccccaaca gtcgctaccg aaa
//
LOCUS       DASUGJ010000010         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1251488, whole genome shotgun sequence.
ACCESSION   DASUGJ010000010 DASUGJ010000000
VERSION     DASUGJ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251488"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /gene="ribB"
                     /locus_tag="VFV93_00335"
     CDS             complement(<1..690)
                     /gene="ribB"
                     /locus_tag="VFV93_00335"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX5163810.1"
                     /translation="MPANTVPEAIAAFRQGRFVIIVDDEDRENEGDLAIAAGAITPEA
                     ITFMAREASGLICVALHPDEVERFGLPPSVPPRRNGTRYGTAFTVSVEAREGVTTGIS
                     AYDRARTIQVLADPEAGPGDLVQPGHIFPLRCHPAGLFARNGQTEASVELALLAGWGG
                     AAVICEVMDDDGRMARMPSLERFSARHDIPIVTIADLIAYLIEPSRRVDHARRFPGAA
                     SVVQRQIEVPLP"
     gene            complement(806..1444)
                     /locus_tag="VFV93_00340"
     CDS             complement(806..1444)
                     /locus_tag="VFV93_00340"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX5163811.1"
                     /translation="MFSGIVEEIGRIGRVSESSAGLTLMIGCRTVLDDVRLGDSIAVD
                     GVCLTVASFSSTSFTANIQPVTARRSSLGSKRSGAPLNLERSLALGGRIGGHYVQGHV
                     DGVGRIAGRRGDGASAIVEIDLPPDIMRYVVERGFIAVDGASLTVMELRPTGIAVSLV
                     AHTQQHITLPALPTGAPVNIETDVIARYVERLVEPREAAGLTIEALQRAGFA"
     gene            complement(1437..2591)
                     /gene="ribD"
                     /locus_tag="VFV93_00345"
     CDS             complement(1437..2591)
                     /gene="ribD"
                     /locus_tag="VFV93_00345"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX5163812.1"
                     /translation="MSFRNVLTDTALMALALDQARSMRGRVAPNPAVGAVVARDGVVI
                     ATGATQPPPGPHAEVVALDLAGAAARGADLFVTLEPCAHTGRTPPCVGAIIAAGIRRV
                     VLAVIDPNPLVAGRGVAALQASGIQAEVGLLAGEAAEIIAGFSRRMATGRPLVTAKYA
                     MTLDGRIATRTGHARWISGPESRAHSHIIRDQIDAIMAGSGTVIADDPLLTTRAEADQ
                     CGQGGPHHPLRVILDSRARTPPSARMLAAATPGRTLIVTGPHAPTDRVAALIAAGAEV
                     VAVDAPDGRVSIPSVLDLLGEQGINDLLVEGGGRVHGAFFDGWLVDRLHIYISPQLVG
                     GEGAKAPVGGLGVATMDGAARLHDPHVERLGDDLFLRGSVVYPEGGTGDV"
     regulatory      complement(2739..2882)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT          533 a         1122 c         1032 g          478 t
ORIGIN      
        1 cggcagcggg acctcgatct ggcgctggac aacgctggcc gcaccgggga agcggcgggc
       61 gtgatcgacg cggcgcgacg gttcgatcag gtacgcgatc aggtcggcga tcgtgacgat
      121 cgggatgtcg tggcgggccg agaaccgttc gagcgacggc atccgcgcca tccggccgtc
      181 gtcgtccatc acctcgcaga tgacggccgc cccaccccag ccggccagca acgccagctc
      241 gaccgacgcc tcggtctggc cgttgcgggc gaacagaccg gccggatggc agcgcagcgg
      301 gaagatgtgt cccggctgga cgagatcacc cggccccgcc tccggatcgg ccaagacctg
      361 gattgtcctg gcccgatcat aggcagagat gccagtcgtc acgccctcgc gcgcctcgac
      421 ggagacggtg aaggccgtcc cgtagcgggt gccgttgcgg cggggcggca cgctcggcgg
      481 caatccgaag cgctcgacct cgtccggatg gagcgcgacg cagatcagcc cgctcgcctc
      541 acgggccatg aaggtaatcg cctcaggcgt gatcgccccg gccgcgatcg ccagatcgcc
      601 ctcattctcg cggtcctcgt catcaacgat gatgacgaac cgtccctgcc ggaacgccgc
      661 aattgcctct ggaactgtgt tggcaggcat gctgttggtc tcctttcata gcgcgtgaaa
      721 cccctcccca accccctccc ccacggggag agggggcttt ggttttcgtg cggccaaaag
      781 cccctctctt ttcaggggag gggtttcacg caaaccccgc tctctgcagc gcttcgatcg
      841 tcaggcccgc tgcctcgcgc ggttcgacaa gacgctcgac gtatctggcg atgacatcgg
      901 tctcgatgtt gaccggcgcg ccggttggga gcgccggcag cgtgatgtgt tgctgggtgt
      961 gggcaaccag cgagacggcg ataccggtcg gacgcagctc catgacggtc aggctggcgc
     1021 cgtcgacggc gatgaagccg cgctcgacga cgtagcgcat gatgtctggt ggcaggtcga
     1081 tctcgacaat ggcggacgcg ccatccccac gccggccggc gattctgccg acgccgtcaa
     1141 cgtggccctg gacgtagtgg ccgccgatcc gtccgcccag cgcgagcgac cgttcgaggt
     1201 tcagtggcgc gccggaccgc ttcgagccga gcgacgagcg gcgggccgtc actggctgga
     1261 tgttggcggt gaagctcgtg ctggagaacg acgcgaccgt caggcagacg ccgtcgaccg
     1321 cgatcgaatc gcccagccgg acatcgtcca gcaccgtccg gcagccgatc atcagtgtca
     1381 acccggcgga tgactcgctc acccgcccga ttcgaccaat ctcttcgacg atgccactaa
     1441 acatcgcccg tacctccctc cggatacacg actgatccgc gcagaaacag gtcatcgcca
     1501 agccgttcga catgcgggtc gtgcaaccgc gccgcgccat ccatcgtcgc gacaccgagc
     1561 ccaccgaccg gcgccttcgc tccctcgccg ccgacaagct gcgggctgat gtagatgtgc
     1621 agccggtcca ccagccagcc atcgaagaac gcgccgtgga cgcgcccgcc gccttcgacc
     1681 agcaggtcgt tgatgccctg ttcgccgagc aggtcgagaa cggatggaat cgacacgcgg
     1741 ccatccggcg catcaacagc cacgacttca gcacctgctg cgatcagagc tgcgacgcgg
     1801 tctgtcggcg catgtggtcc agtcacgatc agcgtccggc ccggtgtggc agctgccagc
     1861 atccgcgcgg atggcggcgt ccgcgcccgg ctgtcgagga tgacgcgcag cgggtggtgc
     1921 ggcccgccct ggccacactg gtctgcctcg gcgcgggtcg tcagcagcgg atcgtcggca
     1981 atgacggtgc cggaaccggc cattatggcg tcgatctggt cgcggatgat gtgcgagtgc
     2041 gcgcgagact ccggcccgct gatccagcgc gcgtggccgg tccgggtcgc gatccggcca
     2101 tcgagcgtca tcgcgtactt ggcggtgacg agcggccggc cagtcgccat ccgccggctg
     2161 aacccggcga tgatctcggc tgcctcaccc gcaagcagcc cgacctcggc ctgaatcccg
     2221 gacgcctgca acgccgcgac cccacggccc gccacgagcg gattcggatc gatgaccgcc
     2281 agcaccaccc gccggatacc ggccgcgatg atggcgccga cacacggtgg ggtccgtccg
     2341 gtgtgagcgc acggttcgag cgtgacgaac agatccgcgc cgcgtgccgc cgccccagcc
     2401 aggtcgagcg cgacgacctc ggcgtgcggc cccggtggcg gctgcgtcgc tcctgtagcg
     2461 atgaccacac cgtcgcgggc gacgacggcc ccgacagccg gatttggcgc aacccgcccg
     2521 cgcatcgacc gcgcctggtc gagcgccagc gccatcaagg cggtatcggt caggacgttc
     2581 cggaacgaca tcgcgggcag cttccactcc gtcaacacaa aaacacccga aggcgaatgc
     2641 cttcggggtg cgtcaacaga gccggatagc accgacacct cggacgcccc acgccatcac
     2701 gacgtggtcc ggccagggac gggcatgctg cgcctgtcgc gagccttctt tcatccggac
     2761 tataccgtcg gccccggagt ttcaccggat catgcaaggc gcttgtcgct cgcctcgctc
     2821 gtgggctgta ccaccgatcg ggaattgggg atcgctcccc gcaccctgcc ccgaaggacc
     2881 tcgctgagcg cagtatacac cggggcgtcg ggaatcggga atcgggattc ggggctggga
     2941 gacatcgaag gcgacccagg cgaacgccgt tccacacccc cgaatcccga atcccgattc
     3001 cccacctctg gcacgccccc tgcgccgtcg ccgtcaaacg gcaagacgac cagcgcatgg
     3061 aggaaacacg ttgacactga tcggctacca cgcctcgcac gagcagttcc ggccgagtga
     3121 gttgctgaaa tatgtccaac tcgccgagca ggccggtttt acggc
//
LOCUS       DASUGJ010000011         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_556925, whole genome shotgun sequence.
ACCESSION   DASUGJ010000011 DASUGJ010000000
VERSION     DASUGJ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556925"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFV93_00350"
     CDS             complement(<1..956)
                     /locus_tag="VFV93_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5163813.1"
                     /translation="MTDSIGTPVLWVAFTGVVLTLLALDLFVFHRKAHTVTLKEAGLW
                     TVGWVSLALAFNAFIYLWAGSDAGLSFTAGYLVELALSVDNMFVFALIFGMFAVPPAY
                     QHRVLFWGILGALVMRFLFILVGAAMLDAFHWIIYVFGAFLVFTGIKILRQREHTLDL
                     EHNVALRLFRRFVPMTSEYYGQRFSIVQAGRRLATPLLAVLVLVEATDLVFALDSVPA
                     IFAITDDPFIVYTSNVAAILGLRSLYFLLAGIMDRFHYLKLGLGAVLVFVGVKMAGAD
                     VYHLPIAASLGVIVALIGGSVVLSLLRPLEETTPHEHPARLEP"
     gene            1267..1626
                     /locus_tag="VFV93_00355"
     CDS             1267..1626
                     /locus_tag="VFV93_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163814.1"
                     /translation="MLVRRTSPALFWITAALLVILLGFGIANLTGLGYPPGDERWRTF
                     VGLFSVCNALFLLAGLLLSDYRSRFGTALLVLAALVAGFTFAPWPIVFQVHALAIVFL
                     AIRRARQRTRPASIVAA"
     gene            complement(1609..2244)
                     /locus_tag="VFV93_00360"
     CDS             complement(1609..2244)
                     /locus_tag="VFV93_00360"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEX5163815.1"
                     /translation="MDTATTQYYFLAGNHCLDYANTADWHEAEQPVEMLTSYGDLLAW
                     GRQAGVLDSSAAERLLRAAEYEPEAAVAVYERAITFREAVFRIFRLIARHETPSASDL
                     ALLNDELRRAGRHRSVAASPEGYEWDWADQDAALDLVLWHVAQSAADLLVEGERERIR
                     QCAGDPCGWLFYDTSRNRSRRWCNMEGCGNRAKARRYYQRRRGQPASGSHD"
     gene            complement(2544..2972)
                     /locus_tag="VFV93_00365"
     CDS             complement(2544..2972)
                     /locus_tag="VFV93_00365"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5163816.1"
                     /translation="MTSKAVEQVEASWAELLSAIDGLDDARMSTPGVTGEWSVKDLLG
                     HIAYWEGRAIAIVERRRNGEPDPTGGSDFDIVNQQVHAERAGWSVAQMLDEFHGTHER
                     FMATLRQYPQIAPDDIAGNTFEHYDEHAAEIRAWRERVGV"
     gene            complement(3051..>4074)
                     /gene="chrA"
                     /locus_tag="VFV93_00370"
     CDS             complement(3051..>4074)
                     /gene="chrA"
                     /locus_tag="VFV93_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX5163817.1"
                     /translation="QYFLDLLGATNLIPGPNSTELVIHSSYQRAGWRGLIVGGSLFIL
                     PAVLLVLGFAWLYVEYGTTTEGEWLLYGIKPVVIAVVAQAIWGLGRTAIKNVYLAAVG
                     LAVLALYLLGLNEIVLLFGAALLVTLVRNGNRLRSGGTRALGLAPWGVLALAPFLAVS
                     AEAKVPYSDSRMFLTFVKIGAVLYGSGYVLLAFLRNDFVDRLGWLTEDQLLDAVAVGQ
                     FTPGPVFTTATFVGYLGGGLSGALVATIGIFLPAFVFVGLSIPLLPRLRRSPWTAAAL
                     DGVNVAAIGLMAAVAWELGRSAIVDLVTIVLALAAALLLIRYRVNSAWLVLGGGLIGI
                     AYKSLI"
BASE COUNT          749 a         1317 c         1356 g          652 t
ORIGIN      
        1 ggctccagcc gggcgggatg ctcgtgcggc gtggtctcct ccagcggccg gagcagcgac
       61 agaacaacag agccgccgat cagggccacg atcactccca gcgaggcggc gatcggcagg
      121 tggtagacat ccgcgccggc catcttgacg ccgacgaaga cgagcactgc gccgagaccg
      181 agcttcagat aatggaagcg gtccatgatc ccggccagca ggaagtagag cgagcgcagg
      241 ccgagaatcg cggcgacgtt cgacgtgtag acgatgaacg ggtcgtctgt gatcgcgaag
      301 atcgccggga ccgagtcgag cgcgaagacc agatcggtcg cttccaccag taccagcacc
      361 gccagcaacg gtgtcgcgag ccgccggccg gcctggacga tcgagaagcg ctggccgtaa
      421 tactccgacg tcatcggcac gaagcgacgg aagagccgca gcgcgacgtt gtgctccaga
      481 tccagtgtgt gctcgcgctg gcgcaggatc ttgatcccgg tgaagaccag gaacgcgccg
      541 aagacgtaga tgatccagtg gaacgcgtcc agcatcgccg cgccgaccag gatgaacagg
      601 aagcgcatca ccagcgcgcc gagaatgccc cagaagagga cgcggtgctg gtaggccggc
      661 gggacggcga acatcccgaa gatcagcgcg aagacgaaca tgttgtcgac cgagagcgcc
      721 agttcgacca ggtagccggc cgtgaacgac agcccggcgt cgctgccggc ccacaggtag
      781 atgaaggcgt tgaaggccag cgccagcgag acccagccga ccgtccagag gccggcttct
      841 ttgagggtca ccgtgtgggc cttgcgatgg aacacgaaca ggtccagcgc cagcagcgtc
      901 aggacgacgc cggtgaatgc gacccagaga accggtgttc cgatcgaatc ggtcatccgc
      961 tatccaactc caaccgcagc agcggtgtac acaatggggg caagtccccc gcggccagtg
     1021 aacgctcacc agtcgcgaag gtcttgcccc caatcaccat cactgggctg ggcgccgggc
     1081 cggagcatct gctcgaaatg acgaccgcgg cacagagggt tgctctgagg ctactcccct
     1141 tcgtccttct attctatgcg gccattctga agcgtggatg aacgccagat gaacgaacgg
     1201 tgattgtcgc gggctggctc gggccgcgtc ggttaggcga tactggaagg ggaatggagg
     1261 agggtcatgc tcgtccggcg cacgtctccg gcgctgttct ggatcacggc cgcgttgctc
     1321 gtcatcctgc tgggattcgg gatcgccaac ctgaccggcc tcggctatcc gcctggcgac
     1381 gagcgctggc gcacgtttgt tggccttttt tcggtctgca atgcgctctt cctgctggcc
     1441 gggctactgc tgagcgacta tcgctcgcgc ttcggaacgg cgctgctggt gctcgcggcg
     1501 ttggtggcgg gctttacctt cgcgccatgg ccgatcgtct ttcaggtaca cgcgctggcg
     1561 atcgtgttcc tcgcgatccg gcgcgccagg cagcggacac ggccggcgtc aatcgtggct
     1621 gcctgatgcc ggctggcccc gtcgtcgctg gtagtagcgg cgagccttgg cgcggttgcc
     1681 gcatccttcc atgttgcacc aacgccggct gcggttgcgg ctggtgtcgt agaagagcca
     1741 gccacaggga tcaccggcac attgccgaat ccgctcccgt tcgccttcga ccagcaggtc
     1801 ggcagccgac tgcgcgacgt gccagaggac gagatcgagc gcggcgtcct gatccgccca
     1861 gtcccactcg tagccttccg gcgacgcggc gacgctccgg tgccggccgg ctcgccgcag
     1921 ttcgtcgttg agcagcgcca gatcgctcgc cgatggcgtt tcatggcgcg cgatcagccg
     1981 aaagatgcga aagacagcct cccggaacgt aatcgctcgc tcgtagacgg cgaccgccgc
     2041 ctccggctca tactcggcgg cgcgcagcaa ccgctcggcc gcactcgaat cgaggacacc
     2101 ggcctgcctg ccccacgcca gcagatcgcc gtacgaggtc agcatctcga ccggctgctc
     2161 ggcctcgtgc cagtcagccg tgttggcgta gtccagacag tggttcccgg ccaggaagta
     2221 gtattgggtt gtcgcggtgt ccatcgagtt gtcctttgtg cgtgacgcgt gacgcgtgat
     2281 acgtgaacca gacgaccggc gtctaggagg aaccgcgccc cttagactgt catcctgagc
     2341 cgcagcgaag gatcgcctgg cgtgaatgag aagagcggtt cggcagagcc actcacgttc
     2401 taacgaacgg cgcttccgtg cctcgtacgt ttagcgatcc ttcgctgcgg ctcaggatga
     2461 caggggtggc gtgtgcggtc ctcacggtcg ccgtccatct gtgtcaccat tctcagcact
     2521 cagcactcag cacgcagcac tcgtcacact cccacccgct cccgccaggc gcggatttcg
     2581 gcggcgtgct cgtcgtagtg ttcgaaggtg ttgcctgcga tgtcgtccgg ggcgatctgc
     2641 ggatactggc gcagggtggc catgaagcgc tcgtgggtgc catggaactc gtccagcatc
     2701 tgggcaaccg accagcctgc ccgctccgcg tgtacctgct ggttcacgat gtcgaagtcc
     2761 gatccgccgg ttgggtcggg ctcgccgttg cggcggcgct cgacgattgc gatcgcccgt
     2821 ccctcccagt atgcaatatg gccgagcaga tccttgaccg accattcgcc ggtgacgccc
     2881 ggcgtgctca tccgtgcgtc gtcgagtccg tcaatcgcgg acagcagttc cgcccagctc
     2941 gcctcgacct gctcgacagc cttgctcgtc atggtgctcc catccgttca agtctaacca
     3001 gtgtcaggat gatagcaggt cataacagct atgtcgatct ggcgggagcg tcagatcagt
     3061 gacttgtagg cgatgccgat caggccaccg ccgaggacga gccaggcgga gttgacgcgg
     3121 tagcgaatca acaggagggc ggcggccagt gcgagtacga tcgtcaccag gtcgacgatc
     3181 gcggagcggc ccagctccca ggcaacggcg gccatcaggc cgatcgcggc gacgttgacg
     3241 ccgtcgagcg cggcggcggt ccacggcgag cgacgcaggc gcggtaggag cgggatcgag
     3301 aggccgacga agacgaaggc cggcaggaag ataccgatcg tcgcgaccag cgcgccgctg
     3361 agcccaccgc cgaggtagcc gacgaaggtg gccgtcgtga agaccggtcc cggcgtgaac
     3421 tggccgacgg cgacggcgtc cagcagttgg tcctcagtca gccagccgag ccgatcgacg
     3481 aagtcgttgc gcagaaacgc caacagcacg tagccggagc cgtagaggac cgcgccaatc
     3541 ttgacgaagg tcaggaacat ccgcgagtcg gagtacggca ccttcgcctc ggccgacacg
     3601 gccaggaacg gcgccagtgc cagtacaccc cacggcgcga gaccgagggc gcgcgtcccg
     3661 cccgagcgca gccggttgcc gttacggacg agcgtcacca gcagggctgc gccgaacagc
     3721 agcacgatct cgttgaggcc gagcaggtac agtgccagaa ccgccagtcc gaccgctgcc
     3781 aggtagacgt tcttgatggc ggtccggccc agcccccaga tcgcctgggc gacgacggcg
     3841 atgacgaccg gcttgatgcc atacagcagc cattcgccct cggtcgtcgt gccgtactcg
     3901 acgtagagcc aggcgaagcc gagcaccagc aacacggccg gcaggatgaa gagcgagccg
     3961 ccaacgatca gcccccgcca gccggcccgc tggtacgacg aatgaatgac cagttcggtc
     4021 gagtttgggc cgggaatcag gttggtggcg ccgagcaggt cgaggaaata ctgc
//
LOCUS       DASUGJ010000012         6037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1391268, whole genome shotgun sequence.
ACCESSION   DASUGJ010000012 DASUGJ010000000
VERSION     DASUGJ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391268"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="VFV93_00375"
     CDS             complement(<1..1377)
                     /locus_tag="VFV93_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX5163818.1"
                     /translation="MRYLALAVDFDGTLAKSGKVTKKTVAALEKLCESGRRLVMVTGR
                     ELPDLVKRFDRLDLFDYVVAENGGLLYHPKTKAETVLGEEPPAELVEALRARGVRKMS
                     VGRSIVAICQPHETTALEVIQELGLEWQIIFNKGAVMLLPASVNKESGLSAALVQLQL
                     SPHNVAGIGDAENDHAFLSLCEGSAAVANALPAVIKRADIVMEGDHGAGVVEFIEMLL
                     DDDLASVARKRTRHDIQFGTLADTKKDVTLKPYGTSILVAGPSGSGKSTATTGFMERL
                     AEQGYQFCLIDPEGDYEGFAGAITFGNQDLAPNVDEALQLLAQPAENVVVNLLGVRLE
                     DRPVFFSRLLPRLQELRARTGRPHWIIIDEVHHLLPRDWKPTDTTIPRQFGEMLMITV
                     HPDMVAPGVLSEVNTIVAAGADPARTFGGFAKALRIKPPKVADGDLRSGQVLLWQRGS
                     RKPPLMVDV"
     gene            complement(1488..2717)
                     /locus_tag="VFV93_00380"
     CDS             complement(1488..2717)
                     /locus_tag="VFV93_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163819.1"
                     /translation="MFDITMTSPQADTAPTTDANHGNRDLRWIGLGLLPLVLLAGVLA
                     LIVATDAGLGDRNVPLIETLSVQRVTLPEPGRITVNVVNDGPDEITIAQVMVDDAYWQ
                     FELDGPRTLDRLESTTIDIPYPWVEGEAHAIGIVSSTGVIFPVEVPVAIESPQANSAT
                     FARFALLGTYVGVIPVALGLLWFPFMRRLGGSAMRFILALTAGLLVFLAVDMFEEARE
                     VALSAPAAFEGPLLVPIVALLAFLLLMTISSRKRDTPRRGLDVAYQIALGIGLHNLGE
                     GLAIGAAFALGEVALGVFLVVGFTLHNVTEGVGIAAPILREQPALRHFAWLALLAGAP
                     AILGVWIGGFVYSPLWTTIFLAIGIGAIVQVVVEVSRIIMRGSARDGQPWLNWTTFGG
                     VTAGIAIMYATALLVAA"
     gene            complement(2738..3856)
                     /locus_tag="VFV93_00385"
     CDS             complement(2738..3856)
                     /locus_tag="VFV93_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX5163820.1"
                     /translation="MPDWKRFNRRDFLRSGAASAAIAGAAGTLGVLIPESGQAEGNDP
                     HPHTSPAQEQTDMDHGGHSMSSTVGDVGPDAIGVDPMDFLTTFDYGTASTLPNGQTLR
                     EYQIVAFDKEIEIAPGIFFPAWTYNGQVPGPTLRCNEGDRLKIHFTNAGTHPHTLHFH
                     GIHSAYMDGSSGIGRGDIQVGETFTYEFDAAPFGCHLYHCHAAPLKRHIHKGLYGTFI
                     INPDPAKHGDLARSRHPDYDESKEWQEFVMVMNAFDTNFDNENDVYAVNSVAFQYMKH
                     PIRVERGRPVRVYLVNVTEFDPINSFHLHATFFHYYDHGTTLEPTLRTIDTVMQCQGQ
                     RGILEMSFAGYEPGLYMFHAHQSEFPELGWMSAFEVVE"
     gene            4018..4995
                     /locus_tag="VFV93_00390"
     CDS             4018..4995
                     /locus_tag="VFV93_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5163821.1"
                     /translation="MPAIEHDIHDHARSKRLTIALAITATVLLAEIIGGLLTNSLALL
                     ADAGHMATDAAALGLALYAIRLARRPATPEHSFGYLRAEILAALVNGASLVAISLYIF
                     WEAFQRLGSPPEVDSGPMLVVAVAGLAANAASAWVLSRGGGHQHDLNTRGALLHVIGD
                     LLGSIGAIVAALVMLATGWYLADPILSAGIGLLILWNSWTLLRESVDVLLEATPSSID
                     AGEVRATMTSVDGVDGVHDLHIWTVTSGLLALSSHVEVTGSRDWHAVLLDLATLLNER
                     FGIAHVTLQPEQTSALPDAFRGCSFDTPEGRAACLDTRTGISVGSGHHH"
     gene            complement(5032..>6037)
                     /locus_tag="VFV93_00395"
     CDS             complement(5032..>6037)
                     /locus_tag="VFV93_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5163822.1"
                     /translation="HCMVKDVELRDNPPNTEGVQLIHRPQEEFDQQIHLYDYATDALH
                     DVLREMRAVLDEFPERVGIGEIHVFDWPKWATYYGQQLDELHLPFNFGLLGIEWDAAE
                     VRALVAELERVIPAGGWPNYVLGNHDEPRIASRIGPEQARIAMMLLLTLRGTPTIYNG
                     DELGMFDVPVALDAVQDPWGRKSGTMGRDPERSPMLWDASPSAGFTTPEATPWLPIED
                     GYEFLNVEAQLADPGSMLTLTRRLLALRRASPALTIGDYLEVASVPAGCLVYERVSGA
                     DRYLIALNFTAESQTLTLPSHPTGSVVLSTHLDRDGEELLMTFDLRPHEGCVVHIAPT
                     "
BASE COUNT         1041 a         1967 c         2005 g         1024 t
ORIGIN      
        1 cacatcgacc atcaacggcg gcttacggct gccacgttgc cagagcagca cctggccgga
       61 tctgaggtcg ccgtccgcga ccttcggcgg cttgatcctg agcgccttcg cgaagccacc
      121 gaacgtcctg gccgggtccg cgccagcggc gacgatcgtg ttgacctcgg agaggacgcc
      181 cggcgcgacc atgtcagggt ggacggtgat catcagcatc tcgccgaact gacgcgggat
      241 ggttgtgtcg gttggcttcc agtcgcgcgg cagcagatgg tggacctcgt cgatgatgat
      301 ccagtgcggg cggccggttc gcgctcgtag ttcctgaagg cgcggcagca gccgggagaa
      361 gaagaccggg cggtcttcga gccggacacc gagcaggttg acgacgacgt tctcggccgg
      421 ctgggccagc agttgcagtg cctcgtcgac gttcggcgcg aggtcctgat tgccgaaggt
      481 gatcgcaccg gcgaagccct cgtagtcgcc ctccggatcg atcaggcaga actggtaacc
      541 ctgctcggcc agccgttcca tgaagccggt cgtcgccgtc gacttgccgc tgcccgacgg
      601 cccggccacc agaatgctgg tgccgtaggg cttgagggtg acgtctttct tcgtgtctgc
      661 gagcgtcccg aactgaatgt cgtgccgcgt tcgcttgcgc gccacgctgg ccagatcgtc
      721 gtccagcagc atctcgatga actcgacgac gccggccccg tgatcacctt ccatgacgat
      781 atcggcgcgt ttgatgacgg ccggcagcgc gttggcgacg gcggccgatc cttcgcagag
      841 gctcaggaaa gcgtggtcgt tctcggcgtc gccaatcccg gccacgttgt gaggcgagag
      901 ctgcagctgc accagcgccg cgctgaggcc actctctttg ttgaccgagg ccggcagcag
      961 catgacggca cccttgttga agatgatctg ccactccagc ccaagctcct ggatcacctc
     1021 cagcgccgtc gtctcgtgcg gctgacagat cgcgacgatc gagcggccga ccgacatctt
     1081 cctgacgccg cgcgcccgga gcgcctcgac cagttcggcc ggcggttcct cgcccagcac
     1141 tgtctcggcc ttcgtcttcg ggtggtacag cagcccgccg ttctcagcga cgacgtaatc
     1201 aaacaggtcg agccggtcga agcgcttcac caggtccggc agctcgcggc cggtcaccat
     1261 caccagccgc ctgcccgact cgcagagctt ctccagcgcc gcgaccgtct tcttcgtcac
     1321 cttaccgctc ttcgccagcg tcccgtcaaa atcgacggcc agcgcgagat atcgcacgta
     1381 tgtcctcttt ccgagtgcag agaattgagc gcaacgtgcc aatcaagagc acgctacgcc
     1441 agcctcaacg caaccggcgt accgaggact gatgtgatcg ggctatgtca cgcagcgacc
     1501 agcagggcgg tcgcgtacat gatggcgata ccggcggtga cgccgccgaa ggttgtccag
     1561 ttgagccagg gctggccgtc gcgggcgctg ccgcgcatga tgatgcgaga gacctcgacg
     1621 acgacctgga cgatcgcgcc gatgccgatt gcgaggaaaa tcgtcgtcca gagcggcgag
     1681 tagacgaagc cgccgatcca gacgccgagg atggccggag cgcccgccag cagcgccagc
     1741 caggcgaagt ggcgcagcgc tggctgctcg cgcaggatcg gcgcggcgat cccgacgccc
     1801 tcggtcacgt tgtggagcgt gaagccgacg acgaggaaga cgcccagcgc gacctcgccc
     1861 agcgcgaagg cggcgccaat cgccagccct tcgccgaggt tgtggagtcc gatcccgagc
     1921 gcgatctgat aggcgacatc cagcccgcgc cggggcgtgt cacgcttgcg cgaggagatt
     1981 gtcatcagca gcaggaacgc cagcagcgcg acgatcggca ccagcagcgg cccctcgaag
     2041 gcggccggcg ccgaaagagc aacctcgcgc gcctcctcga acatgtcgac ggccaggaag
     2101 acgagcagac cggccgtcag cgccagaatg aagcgcatcg cactaccacc cagccggcgc
     2161 atgaatggga accacagcag ccccagcgcg accgggatga cgccgacata ggtcccaagc
     2221 agcgcgaagc gggcgaaggt cgccgagttg gcctgtggcg attcgatggc gaccggcact
     2281 tcgaccggga agatgacgcc ggtcgacgag acgatcccga tcgcatgcgc ctcgccctcg
     2341 acccacgggt aggggatatc gatcgtcgtc gattcgagcc ggtcgagcgt ccgcgggccg
     2401 tccagctcga actgccagta ggcgtcatcg accatgacct gggcgatcgt gatctcgtcc
     2461 ggcccgtcgt tgacgacgtt gacggtgatc cggcccggct cgggcagcgt gacgcgctgg
     2521 acgctgagcg tctcgatcag cggcacgttc cgatcaccaa gcccggcgtc ggttgccacg
     2581 atcagcgcca gcactccggc cagcagcacg agcggcagca gccccagccc gatccagcgc
     2641 aggtcacggt tgccatggtt cgcgtccgtt gtcggggcgg tatccgcctg tggactggtc
     2701 atggtgatat cgaacatcaa gtgctcctca gccccgtcta ctcgacgacc tcgaacgcgc
     2761 tcatccagcc cagttcgggg aactcggact ggtgggcgtg gaacatgtac agccctggct
     2821 cgtagccggc gaacgacatc tcgagaatgc cgcgctgtcc ctgacattgc atgacggtgt
     2881 cgatcgtccg caacgtcggc tccagcgtcg ttccgtggtc gtaatagtga aagaacgtcg
     2941 cgtgcaggtg aaatgagttg atcggatcga actcggtcac gttgaccaga tacacccgca
     3001 ccggccggcc gcgctcgact cggatcgggt gcttcatata ctgaaaggcg acgctgttga
     3061 cggcgtagac gtcattctcg ttgtcgaagt tcgtgtcgaa agcattcatc accatcacga
     3121 actcctgcca ctccttcgac tcgtcataat cgggatggcg tgagcgggcc agatcaccat
     3181 gtttggcagg gtcggggttg ataatgaacg tgccgtagag tcccttgtgg atgtggcgct
     3241 tgagcggggc ggcgtggcag tgatacaggt gacagccaaa cggggcggcg tcaaactcgt
     3301 aggtgaaggt ctcgccgacc tggatatcgc cacggccgat accggaagat ccgtccatgt
     3361 aggcggagtg aatgccgtga aagtggagcg tgtgtgggtg cgtaccggcg ttggtgaagt
     3421 ggatcttcag cctgtcgccc tcgttacaac gcagggtcgg ccccggcacc tggccgttgt
     3481 aggtccaggc cgggaagaag atgccgggcg cgatctcgat ctccttgtcg aaggcgacga
     3541 tctggtactc gcgcagcgtc tggccgttcg gcagcgtgct ggcggtgccg tagtcgaacg
     3601 tcgtcaggaa gtccatcggg tcgaccccga tcgcgtccgg cccgacatcg ccgaccgtcg
     3661 aagacatcga gtggccgccg tggtccatgt cggtttgttc ctgggcaggg ctggtgtgcg
     3721 ggtggggatc gttcccttcc gcctgaccgg actcggggat caacaccccc agcgtcccgg
     3781 ccgccccggc gatggccgcg ctggccgcgc cagagcgcag gaaatcccgt cggttaaatc
     3841 gcttccagtc cggcattcga ctcccagtgt ccaatcacga caacgatgac ctctcataat
     3901 gatattaggc ttgcctaatt tgtcaatgag ccggtacgcc gtgagcggtg aggcagcgcg
     3961 aagcgcggtc ggccgcggta ctctgcatgc gagaatcgcg agtggatggg agggcaattg
     4021 ccggcaattg agcacgacat ccacgatcac gccagatcaa agcggctgac gatcgcgctc
     4081 gcgatcacgg ccaccgttct gctggcggag atcatcggtg gactcctgac caactcgctg
     4141 gcgttgctgg ccgacgccgg ccacatggcg accgatgcag ccgctttggg actggcgctg
     4201 tacgcgatcc ggctcgcgcg gcggccggcg acgcccgagc actcgttcgg ctacctgcgg
     4261 gcggagatcc tggcggcact cgtcaacggc gcctcgctgg tcgccatctc gctctacatc
     4321 ttctgggagg cattccaacg gctcggctcc ccgccggagg tggacagcgg cccgatgctc
     4381 gtggtggccg tcgccgggct ggccgccaac gcagcctcgg cctgggtcct ctcgcgtggc
     4441 ggcggccacc agcacgatct gaacacacgc ggcgcgctgc tacacgtcat cggcgacctg
     4501 ctcgggtcga tcggtgcgat cgtcgccgcc ctcgtcatgc tggcgaccgg ctggtatctg
     4561 gccgacccga tcctctcagc cgggattggg ctgttgatcc tctggaattc gtggacgctg
     4621 ctgcgcgaat cggtcgatgt gctgctggag gcgacgccat ccagcatcga cgcgggcgaa
     4681 gttcgggcca cgatgacgtc cgtcgatggc gtcgacggag ttcacgacct gcacatctgg
     4741 acggtcacct ccggcctgct cgcgctgagc agccacgtcg aggtgaccgg cagccgcgac
     4801 tggcacgccg tcctgctcga cctcgcgacg ttgctcaacg agcgcttcgg catcgcccac
     4861 gtcacactcc agccggagca aaccagcgcc ctgccggacg catttcgcgg ctgcagcttc
     4921 gacacgccgg aaggaagagc cgcctgcctc gacacgcgca cgggcatatc cgtgggaagc
     4981 gggcaccatc actagcacca gcgcagcaca gccgcccggc gcgacacgca atcacgtcgg
     5041 cgcgatgtgg acgacgcatc cttcgtgcgg acgcagatcg aaggtcatca gcagttcctc
     5101 accgtcgcgg tcgagatggg tgctgaggac gaccgagccg gttggatgag atggaagcgt
     5161 cagcgtctgc gactcggcgg tgaagttgag cgcgatcagg tagcggtcgg cgccagatac
     5221 gcgctcgtaa acgaggcagc cggccgggac gctggcgacc tccaggtagt cgccgatcgt
     5281 cagcgccggg ctggcgcggc ggagggcgag caggcggcgg gtgagcgtca gcatcgaacc
     5341 gggatcggcc agctgcgcct cgacgttcag gaactcatag ccgtcttcga tcgggagcca
     5401 gggagtcgct tccggcgtcg tgaagccggc gctcggtgac gcgtcccaga gcatcggtga
     5461 gcgttcgggg tcgcggccca tcgtgccgga cttgcgcccc cacggatcct gaacggcgtc
     5521 gagggcgacc ggcacgtcga acatgcccag ctcgtcgccg ttgtagatcg tcggcgttcc
     5581 gcgcagggtc aacaacagca tcatcgcgat gcgggcctgt tccgggccga tgcggctggc
     5641 gatacgcggt tcgtcgtgat tgccgaggac gtagttcggc cagccaccgg ccgggatgac
     5701 ccgctccagc tcggcaacca gcgcgcggac ctcggccgcg tcccactcga tcccgagcag
     5761 gccgaagttg aacggcaggt gtaactcgtc gagctgctgg ccgtagtacg ttgcccactt
     5821 cggccagtcg aagacgtgga tctcgccaat cccgacccgt tcggggaact catcgaggac
     5881 tgcgcgcatc tcgcgcagga cgtcatgcag cgcgtccgtc gcgtagtcgt agaggtggat
     5941 ctgctggtca aactcttcct gcgggcggtg gatcaactgg acgccctcgg tgttgggcgg
     6001 gttgtcgcgc agctcgacat ccttgaccat gcagtgg
//
LOCUS       DASUGJ010000013         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1808018, whole genome shotgun sequence.
ACCESSION   DASUGJ010000013 DASUGJ010000000
VERSION     DASUGJ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808018"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VFV93_00400"
     CDS             <1..839
                     /locus_tag="VFV93_00400"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5163823.1"
                     /translation="IARRLLAAGYPLAVNNRSQASVQELAGLGAAALPDAAAIGAACD
                     VVLTALPTPETVHEVYLGERGLLAGASGDTLLIDLSTVSPALSREIAAAARQRGLEFL
                     DAPISGGPEGAVNGTLTIMCGGDGTAFERARPIFDVFGERIYHAGPSGAGCTIKLVNQ
                     LLVSIHAVATAEALALARQAGADSELTFEVVRNAWGNSRIFERSFPLMLAGNDDGGAN
                     ISIVLKDITVILDLARELGLPLGVGSAAGQALTTARDQGLGARDIAALRLLFPGAAAD
                     SD"
     gene            941..2017
                     /gene="ada"
                     /locus_tag="VFV93_00405"
     CDS             941..2017
                     /gene="ada"
                     /locus_tag="VFV93_00405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEX5163824.1"
                     /translation="MMTIDTTAGLMTDEERWLAFEARDTGYDGRFVVAVRSTGIYCRP
                     SCPARRPLRRNVVFYPAGDAAEREGYRPCRRCKPEEVLPQARAIEQASRYIAEHLDRN
                     VTLNELGEAVGVSPYHLQRTFKRVMGISPRAYADALRLGSVKSSLRDGGDVTTALYDA
                     GYGSSSRLYERAPAQLGMTPGAYRRGGQGMSIGYTIVDSPLGRLLVGATEQGVSAVSL
                     GDDDATLEQALRAEYPAAEIARDDSRLGRWTTAIVAHLSGRLPHLDLPLDLQATAFKR
                     RVWEALREIPYGETRGYAEIARQIGRPTAVRAVANACDTNPAAIVIPCHRVVRSDGAP
                     GGYRWGEQRKQALLARESRDAQLG"
     gene            2122..2874
                     /locus_tag="VFV93_00410"
     CDS             2122..2874
                     /locus_tag="VFV93_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5163825.1"
                     /translation="MEWISSPEIWVALLTLTALEIVLGIDNIIFISILVAKLPKEMQQ
                     RARLIGLSAAMIMRILLLFSLSWIIGLEDPWFTILDQEISGRDLILVVGGLFLLGKAT
                     YEIHEKLEGVEGHTSTRVAPSFVSVIIQIMLLDIVFSIDSVITAVGMADDVEVMIAAV
                     IIAIIVMMVAAKSISEFVDAHPTVKMLALSFLLLIGMSLVAESFDQHIPKGYIYFAMG
                     FSVFVEMLNLRLRGGDKQPVHLHDGYTGVGDA"
BASE COUNT          448 a         1016 c          952 g          484 t
ORIGIN      
        1 cgatcgcacg gcggctgctg gcggccggct acccgctggc ggtcaataac cgcagtcagg
       61 cgagcgtcca ggaactggcc ggactcgggg ctgccgcgct accggatgcc gccgcgatcg
      121 gcgcggcttg cgacgtcgtc ctgacggcgc tgccgacgcc ggagaccgtc cacgaggtct
      181 atctcggcga acgcgggctg ctggccggcg cgagtggtga cacgctgctg atcgacctct
      241 cgaccgtcag cccggccctg agccgcgaga tcgcggcagc agcccgccag cgaggactgg
      301 agttcctcga cgcaccaatc tccggtgggc cggagggcgc cgtcaacggt acgctgacga
      361 tcatgtgcgg cggggatggc accgccttcg agcgggcgcg gccgatcttc gacgtcttcg
      421 gcgagaggat ctaccatgcc gggccgtccg gcgcgggctg cacgatcaag ctcgtcaacc
      481 aactactcgt ctcgatccac gccgtcgcga cggccgaggc gcttgccctg gcgcggcagg
      541 ccggcgccga ttcggaattg acgttcgagg tcgtccgcaa tgcctggggc aactctcgca
      601 tcttcgagcg ctcgttcccg ctgatgctgg ccggcaacga cgatggcggc gcgaacatca
      661 gcatcgtcct caaggacatt accgtcattt tggatctggc gcgcgagctt ggcctgccgc
      721 ttggcgtcgg ctcggccgcc ggtcaggcgc tgacgaccgc ccgcgaccag gggctcggcg
      781 ctcgcgatat cgccgcgctc cggctgctgt tccccggcgc tgccgcagat tccgattgag
      841 cgcaatcgcc ggatagccgt gccggccgct ctcccctacg ctagagacag gccagccgaa
      901 ctggccggcg ctcgatcgcg gatagcccaa aggacgcagc atgatgacga ttgacacgac
      961 agcaggcctg atgaccgacg aggaacgctg gctggcgttc gaagcccgcg acaccggcta
     1021 cgacggacgc ttcgtcgtcg ccgtccggag caccggtatc tactgccgtc catcctgccc
     1081 ggcccggcgg ccgctacgcc ggaacgtcgt cttctacccg gccggcgacg ctgccgagcg
     1141 cgaaggctac cggccctgcc gccgctgcaa gcccgaggag gtgctgccgc aggcgcgcgc
     1201 gatcgagcag gccagccgct acatcgccga gcacctggac cgcaacgtga cgctcaacga
     1261 actgggcgag gccgtcggcg tctcgccgta ccacctccag cgcacgttca agcgggtgat
     1321 gggcatctcc ccgcgtgcct atgccgatgc cctccggctc ggctcggtca agtccagcct
     1381 gcgcgatggc ggcgacgtga cgactgccct ctacgatgcc ggctatggct ccagcagccg
     1441 cctctacgag cgcgcccccg cccagctcgg gatgaccccc ggcgcctacc ggcgtggcgg
     1501 gcagggcatg tcgatcggct acacgatcgt cgatagcccg cttggccggc tgctggtcgg
     1561 cgcgacggaa cagggcgtct cggccgtctc gctcggtgac gatgacgcca cgctggaaca
     1621 ggcgctgcgg gccgaatacc cggccgccga gattgcccgc gacgacagcc gcctcggccg
     1681 ctggacgacc gcgatcgtcg cccatctctc cggtcggctg ccccatctcg atctgccgct
     1741 cgacctgcag gcgaccgcct tcaagcgccg cgtctgggag gcgctgcgcg agatcccgta
     1801 cggcgagaca cgcggctacg ccgagatcgc ccgccagatc ggccggccga cggcagtccg
     1861 cgccgtcgcc aacgcctgcg acaccaatcc ggccgcgatc gtcatcccct gccaccgcgt
     1921 cgtccgctcc gacggcgccc ccggcggcta ccgctggggc gagcagcgta agcaggcgtt
     1981 gctggcgcgg gaatcgcggg acgcgcagct cggctaatcc gcgtcccgcg tcccgcgtcc
     2041 cgccctgaca tacaatcaca cgaacgcatc gtcctcggtg gcgatgcgtt cgtgtatctg
     2101 cgtgtcacga gaggggtgcg ggtggagtgg atatcgagtc ctgagatctg ggtcgcgctg
     2161 ctgacattga cggcgttgga gatcgtcctc ggtatcgaca acatcatctt catctcgatc
     2221 ctggtcgcca agctcccgaa ggagatgcag cagcgggcgc ggctgatcgg tctcagcgcg
     2281 gcgatgatca tgcggattct gctgctcttc tcgctgtcat ggatcatcgg gctcgaagat
     2341 ccgtggttca cgatcctgga tcaggagatc tccgggcgcg acctgatcct tgtggtcggc
     2401 ggcctcttcc tgctcggcaa ggcaacgtac gagatccacg agaagctcga aggcgtcgag
     2461 ggccacacca gcacgcgggt cgcgccgtcg ttcgtgagcg tcatcatcca gatcatgctg
     2521 ctcgacatcg tcttctcgat tgactcggtc atcaccgctg tcgggatggc cgacgatgtc
     2581 gaggtcatga tcgcggccgt catcatcgcc atcatcgtca tgatggtcgc cgccaagtcg
     2641 atcagcgagt tcgtggacgc gcacccgact gtcaagatgc tggccctctc gttcttgctg
     2701 ctaatcggca tgtcgctcgt cgccgagtcg ttcgaccagc acatcccgaa gggctacatc
     2761 tacttcgcga tgggcttctc cgtcttcgtc gagatgctca acctgcgcct gcgcggcgga
     2821 gacaagcagc cggtccatct gcatgacggg tatacggggg tgggtgacgc gtgacgcggg
     2881 acgcgttacg aaccccgcat
//
LOCUS       DASUGJ010000014         5327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_418936, whole genome shotgun sequence.
ACCESSION   DASUGJ010000014 DASUGJ010000000
VERSION     DASUGJ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418936"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VFV93_00415"
     CDS             <1..1446
                     /locus_tag="VFV93_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5163826.1"
                     /translation="EEREHGLATIDAITRAGETASKAVLFSGMTVVVSLLGMLIVPWN
                     LMTSVGGAILVVLTSVVMTLTLLPAVLSLLGDRVNRGRIPFIGYRRATTSTSSDSASR
                     FWTSISRLVMRRPVVSLIASAGLLLILGSFTFSLHLGHGTLSNLPENSDSIQAVQIFE
                     NEFPGADYQPATIVVEADDVTTPAIQTAINNLLATLDSDPAFGPATLATSANHDLVRI
                     DVALKVDEEGNQAIDAIKRLRGDYVPALFGSTGASVHVTGETASMIDIIDLARTYLPI
                     VVAFVLAISFLLLLVAFRSIIVPLKAITMNLLSVFASYGLLVLFFQKGVGADLLGFQQ
                     VDRIDAFVLLFLFCILFGLSMDYHVFLLSRIKEQFDITGDNEQAVTAGLRSTGKLITS
                     AALIMVGVFGGFAAADLANLQQFGFGLAAAVFLDATVVRMVLVPASMALLGNRNWYLP
                     AWLNWLPTMHIEGTPAHLPAEQPVFEPVLAD"
     gene            complement(1627..2010)
                     /locus_tag="VFV93_00420"
     CDS             complement(1627..2010)
                     /locus_tag="VFV93_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5163827.1"
                     /translation="MATVNVRYIVNDVDDAIAFYTQHLGFQVDMHPGPGFARISRGDL
                     ALLLNTAGGGGGAGQSMPDGRRPEPGGWNRIQLEVDDLAAVVDPLRQAGAQFRNEIVT
                     GNGGKQILLEDPSGNPIELFEPFAR"
     gene            2211..2744
                     /gene="cpt"
                     /locus_tag="VFV93_00425"
     CDS             2211..2744
                     /gene="cpt"
                     /locus_tag="VFV93_00425"
                     /inference="COORDINATES: protein motif:HMM:NF019544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol phosphotransferase CPT"
                     /protein_id="HEX5163828.1"
                     /translation="MTTQVIVLNGGSSSGKSGIARCLQAILPQPWISVSIDDLVDALP
                     PSMTDSGAGIAFGQAGEVSVGDDFRQIEAAWLTGIAAMARAGARVIIDDVFLSGAASQ
                     QRVRAQLDGLDVLWVGVRCDPNIAAGREIARGDRTVGMAALQAETVHQGVTYDVEVDT
                     SQSESLDCARTIAARVN"
     gene            complement(2852..5002)
                     /locus_tag="VFV93_00430"
     CDS             complement(2852..5002)
                     /locus_tag="VFV93_00430"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5163829.1"
                     /translation="MQQGDSRLLTLNGAASSDHRGGTVRTQAGDDEQGEVAEPLQPWL
                     GPATNGLHESERLLIGRKWLDVEDQNRILEMMTQGASLADGLDLLVHSVETQSANPLR
                     ASILLLDAAGTHLRHGAAPSLPDAYNAAIDGLAIGPNAGSCGTAAFTGVPVVVSDIAT
                     DPLWADYRDLALLHDLRACWSMPILSAAGGILGTFAIYYDEPRSPSDEDKQLIVFAAR
                     TAALLIERRRSDAALAESERRFRAMIDALPAAIYTTDADGWLTHFNPAAVAFSGRVPK
                     LGSDRWCVSWKLYYPDGTPMRHDECPMAVAIKEGRVIQGVEAIAERPDGSRVWFMPYP
                     TPLRDPDGRIVGGINMLIDITERKRAEEMRARLAAIVESSDDAIVSKDLDGVIMSWNG
                     GAERLFGYTPEEAIGQSITILIPPDRMDEEPEILGRIRRGERIDHFETVRQHKDGTLL
                     DISLTVSPMVDGHGRIVGASKIARNITERRALERQKDAFLGIAAHELRTPVTGIKAYT
                     QLLARRMRKAGDASSAETLDKLDAQADRLTGLIGDLLDVTRIQGGMLPLRPAPFDLDT
                     LLHEVIDEAQRSTSQHQIVAELAAPVTIAGDRDRIGQVVMNLLTNAIKYSPDAECVIV
                     RSARDGENVVVAVQDFGIGIPKQDQPRIFERFYRVTSASRAGYAGMGLGLNIASEFVK
                     RHAGTIAVDSEEGEGTTITFSLPIAGPRDEDPAI"
BASE COUNT          893 a         1842 c         1666 g          926 t
ORIGIN      
        1 gaggaacgcg agcacggctt ggccacgatc gacgccatca cccgcgccgg tgagacagcc
       61 agcaaggccg tcctcttctc cggcatgacg gtcgtcgtct cactgctggg catgctgatc
      121 gtgccctgga acctgatgac cagcgtcggc ggcgcgatcc tggtcgtcct gacctcggtc
      181 gtgatgacac tcacgctgct gccggccgtc ctcagcctgc tgggcgaccg ggtcaaccgc
      241 ggccggatcc cgttcatcgg ctaccggcga gcgacgacca gcacgagcag cgattccgcc
      301 tcgcgcttct ggacctcgat ctcgcggctc gtcatgcgcc ggccggtcgt cagcctgatc
      361 gcctcagccg gtctgctgct gatcctcggc agcttcacct tctcgctgca cctcggccac
      421 ggcaccttgt ccaacctgcc agagaactcc gacagcatcc aggccgtcca gatcttcgag
      481 aacgagttcc ccggcgccga ctaccagccg gccacgatcg tcgtcgaggc cgacgacgtc
      541 accaccccgg cgatccagac ggccatcaac aacctgctcg cgacgctcga cagcgacccc
      601 gccttcggcc cggccacgct ggcgacctcc gccaaccacg atctggtgcg catcgacgtc
      661 gcgctgaagg tcgacgaaga gggcaaccag gcgatcgacg cgatcaagcg actgcgcggc
      721 gactacgtcc cggccctgtt cggctcgacc ggcgccagcg tccacgtgac cggcgagacg
      781 gcgtcgatga tcgatatcat cgacctggcc cgcacctacc tgccgatcgt cgtcgccttc
      841 gtcctcgcga tcagcttcct gctgctgctg gtggccttcc gctcgatcat cgtcccgctc
      901 aaggccatca cgatgaacct gctctcggtc ttcgcctcct acggcctgct ggtgctgttc
      961 ttccagaagg gtgtcggcgc ggacctgctc gggttccagc aggttgatcg gatcgatgcc
     1021 ttcgtcctgc tgttcctgtt ctgcatcctg ttcggcctct cgatggacta ccacgtcttc
     1081 ctgctgagcc gcatcaagga gcagttcgac atcactggcg acaacgagca ggccgtcaca
     1141 gccgggctgc gctcgaccgg caagctgatc accagcgcgg ccctgatcat ggtcggtgtc
     1201 ttcggtggct tcgcagctgc cgacctcgcc aacctgcaac agttcggctt cggcctggca
     1261 gccgccgtct tcctcgacgc aacggtcgtc cggatggtgc tcgtccccgc ctcgatggcc
     1321 ctgctcggca accgcaactg gtacctgccg gcctggctga actggctgcc gacgatgcac
     1381 atcgaaggca cgccggccca cctcccggcc gaacagcccg tcttcgaacc agtcctcgcc
     1441 gactgagcga tcgcctcccg catcacgaac gcgatccttc aatgcaaatg agaccagcac
     1501 cgggttggtg ctggtctcgt tcgtcttcgt gccggggaac cgctggactg gacagcgtcc
     1561 tcaacgacag gtctgtgatc cgatctacta tccggcaatc acctggcagc caatccccgc
     1621 ctgcgactag cgagcgaatg gctcgaacag ctcgatcgga ttaccggacg gatcctccag
     1681 cagaatctgc ttgccgccgt tgccggtcac gatctcgttg cggaactgcg ccccagcctg
     1741 tcgtagcggg tccacgaccg cggccagatc gtcgacctcg agctggatcc gattccagcc
     1801 gccaggctcc ggacgtcgtc catccggcat cgactggccg gcgccgccac cacccccggc
     1861 cgtgttcaac aggagcgcga ggtcaccacg ggaaatacgc gcgaagccgg ggccaggatg
     1921 catatcgacc tgaaatccca gatgctgggt atagaacgcg atcgcatcgt cgacgtcgtt
     1981 cacgatgtag cggacattga ccgttgccac gcttccgtct cctttcctgg ggcgacgtta
     2041 cggctcggat cgttccacat cgccagattg ttgaccaccc agccggagga atgtctcagt
     2101 gccatagctc ccgacacggt ggtctggaac actcaggcaa cggcgcggca cgtacgactg
     2161 gttgcatcgc tgagcgagcg aaccagacac cgggaaggca aaccagcagc atgacgacac
     2221 aggtcatcgt cctcaacggc ggttccagct ccggcaagag cgggatcgcc cgctgcctgc
     2281 aggcgatcct gccgcagcca tggatcagcg tcagcatcga cgacctggtc gatgcgctgc
     2341 cgccgtcgat gacggactca ggggccggca ttgcgttcgg acaggcgggc gaggtctcgg
     2401 tcggcgacga cttccgccag atcgaggctg cctggctgac cggcattgcc gcgatggcgc
     2461 gggctggggc gcgcgtcatc atcgacgatg tcttcctcag tggcgcggcc tcccagcagc
     2521 gcgtccgcgc ccaacttgac gggctcgacg tactctgggt cggcgtccgc tgcgacccca
     2581 acatcgcggc cggccgcgag atcgcccgcg gcgatcggac ggtcgggatg gccgccttgc
     2641 aggcggagac agtccatcag ggcgttacct acgacgtcga ggtcgacacc agccagtccg
     2701 aatcactgga ctgcgcccgc actatcgccg cgcgtgtcaa ctgacgcgac ccggtagggg
     2761 cgaggcaccg actcgcccgt cgtgtcccgc ccaaacggct gcggtgggga gccgggcgag
     2821 ttggtgtctc gcccctaccg gtgagaccga atcagatggc ggggtcttcg tcgcgcggac
     2881 cggcgatcgg caacgagaag gtgatggttg taccctcgcc ctcctcgctg tcgactgcga
     2941 tcgtcccggc gtggcgcttg acgaactcgg acgcgatgtt caggccaagt cccatcccgg
     3001 catagccggc gcggctggcg gacgtgacgc ggtagaagcg ctcgaagata cgcggctggt
     3061 cttgcttcgg aatcccgatc ccgaaatcct ggacggccac gacgacgttc tcgccgtcgc
     3121 gcgccgagcg gacgatgaca cattccgcat ccggcgagta cttgatcgcg ttggtcagca
     3181 ggttcatcac gacctgcccg atccggtcgc ggtcgccggc gatcgtgact ggcgcggcta
     3241 gttcggcgac gatctggtgc tggctggtgg atcgctgagc ctcgtcgatg acctcgtgaa
     3301 gcagcgtgtc gaggtcaaac ggagccgggc ggagcgggag catgccaccc tgtatccgcg
     3361 tcacgtcgag caggtccccg atcaatcccg tcagccggtc ggcttgcgcg tcgagcttgt
     3421 cgagcgtctc cgccgaactg gcatcacccg ccttccgcat gcgacgggca agcagttggg
     3481 tgtaggcctt gatgccggtg accggcgtgc gaagctcgtg ggcggcgata ccgaggaagg
     3541 cgtctttctg ccgctccagc gcccgccgct cggtgatgtt gcgggcgatc ttcgaggcgc
     3601 cgacgatccg gccatgccca tccaccatcg gcgagacggt cagcgagatg tcgagcagcg
     3661 tgccatcctt gtgctggcgg acggtttcga agtggtcgat gcgctcaccc cgccggatcc
     3721 gtcccagaat ctccggctct tcgtccatgc ggtcgggcgg aatcagaatc gtgatcgact
     3781 ggccgatcgc ctcttccggc gtgtaaccga acagccgttc tgcaccaccg ttccacgaca
     3841 taatgacgcc gtcgaggtcc ttgctgacga tggcatcatc ggacgactcg acgatcgcgg
     3901 ccagccgggc gcgcatctcc tcggcccgct tgcgttcggt gatgtcgatc agcatgttga
     3961 tgccaccgac gattcggcca tcggggtcgc gtagcggtgt cgggtagggc atgaaccaaa
     4021 cccgcgaacc gtcagggcgc tcggcaatcg cctcgacgcc ctgaatgaca cgaccctcct
     4081 tgatcgcaac cgccatcgga cactcatcgt ggcgcattgg cgtaccatct ggatagtaga
     4141 gcttccagct cacacaccag cgatcgctac ccagtttcgg cacgcggccg gagaatgcga
     4201 cggcggccgg gttgaagtga gtcagccagc cgtcggcgtc ggtcgtgtag atcgcggccg
     4261 gtagcgcgtc gatcatcgcc cggaagcgcc gctcgctctc ggccagggct gcgtcggagc
     4321 gccgccgttc gatcagcagg gcggccgtcc gcgcggcgaa gacgatcaac tgcttgtcct
     4381 catcgctcgg gctgcgcggc tcgtcgtagt agatcgcgaa cgtcccgaga atcccgccgg
     4441 ccgccgacag gatcggcatc gaccagcagg cgcgcaggtc gtgcagcagc gccaggtccc
     4501 tgtagtcggc ccataacgga tcggtggcga tatccgagac gacgaccggc acgccggtaa
     4561 aggccgccgt gccgcaggaa ccggcgttcg gcccgattgc gagaccgtcg atggccgcgt
     4621 tgtaggcgtc gggcaggctc ggcgccgcgc cgtgccggag gtgcgtcccg gcagcatcga
     4681 gcagcaggat cgacgcgcgt aacgggttcg cggactgcgt ctcgacactg tgcaccagca
     4741 ggtcgagccc gtccgccagc gaggccccct gggtcatcat ctccaggatg cggttctggt
     4801 cctcgacgtc cagccacttg cgaccgatca gaagcctctc cgattcatgg agcccgttcg
     4861 tcgccgggcc gagccatggc tgcaacggtt cagccacctc gccctgctca tcatcgccag
     4921 cctgggtacg gacagtgccc ccacggtgat cggacgatgc cgcgccgttg agagtgagaa
     4981 gccgcgagtc tccttgctgc atcgccatgt tactcctgaa tgcgtttatc cgcagcctca
     5041 ctggtcacat caagcgagga cacaaccatc gccacccacc aattgacacc atcgacccat
     5101 gcagatggcg ttcggcaggc gtcacacgcg ccgcccggaa aacacggcaa cctgtgccat
     5161 cagctgggta tcgagatcag aatgcaagaa gcgtgcccgg ggcggatgcc gatcggctca
     5221 acgctgccct cggcagagac actggtgtct cagaatcgct ggaccaataa acggggcagg
     5281 tcagcggcca gctcgttcgc ggtcacggtc gctgccgggc cgcgccg
//
LOCUS       DASUGJ010000015         4089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_279848, whole genome shotgun sequence.
ACCESSION   DASUGJ010000015 DASUGJ010000000
VERSION     DASUGJ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279848"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFV93_00435"
     CDS             <1..912
                     /locus_tag="VFV93_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163830.1"
                     /translation="VIGSTVNPFATGIASGFADVSISDGLWMRVLVLLIGLVIGVFFV
                     MRYAAHVKEDPSKSLIYAMKAENEARWLAGQGDSDDFGTLTGRRKLVLVFFFLAFAVM
                     IYGVIPWEDLGVGIPTLWWWFPEMTASFILFAILIGMIGRLSEKDLTDSFVNGSRDLL
                     GVALIIGIARGITVIMNNGMITDTVLNWTEEAVADLGGAKFVLLLFILFVPLSFLIPS
                     SSGLATVAMPIMAPLALFAGINGELVVSAYQFGSGLVALVTPTSAVVMGGLAIARVGY
                     GTWLKFVWPLLIALTALCALALAIGAA"
     gene            979..1980
                     /gene="argF"
                     /locus_tag="VFV93_00440"
     CDS             979..1980
                     /gene="argF"
                     /locus_tag="VFV93_00440"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5163831.1"
                     /translation="MSIGLKGKSLLDVGELSKEQFVALLDLSSELKAAKRNGTEQPLL
                     KQKEIVLIFEKTSTRTRCAFEVAAYDQGAHVTYLGPEGSQIGHKESMKDTARVLGRMY
                     DAIEYRGFSQELVEILSTHAGVPVYNGLTDEFHPTQMLADMLTMREHSGKPLERIAYA
                     YMGDAHSNMGRSLLETGAILGMDVRIIAPREIWPDEAYIAQHRQRGATSGARITVTDD
                     IAAGVKGVDFIHTDVWVSMGEAHEVWVERIELLRPYQVNMDVIRQTGNPDVKFMHCLP
                     AFHNRETKVGEEIYEKFGLDCMEVTEEVFESPRSIVFDQAENRMHTIKAILVATLAG"
     gene            2041..3573
                     /gene="nhaC"
                     /locus_tag="VFV93_00445"
     CDS             2041..3573
                     /gene="nhaC"
                     /locus_tag="VFV93_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEX5163832.1"
                     /translation="MASDTSSTSTSRPPRPPSMLDAVIPVVALIALISLSVYLYGVDS
                     TNGPLQVSIFTAAVVAGLVAHKNGYAYADLGDAAIGGISTAMGAIFILLAVGALIGTW
                     NMAGTIPTIVDYGIRLLDPAWFYLAVALICAITGAVTGSSWTTAGTLGVAFVGMAHII
                     GVNPEITAGAVISGGYFGDKMSPLSETTILTPSLVGGLTTGQHIRAMAWSTGPAFAIS
                     AVLFLIISLNTDVSSSTAEIDEARAVLNDSFNISIINLLPVALLIAFSVKKFPAFLSI
                     FLTALFSGVLAGFTQRDQVIQFANDPDLSTPLAILKGIYGSMATGYVSSTGNEGVDML
                     FSRGGMSSMLSTVWIILGALAFGAIMEHAGFLNRLVETLLEKATSGGALVASVIGTAI
                     GLNIIAADQYIAIVLPARSFRVEFRRRGYQPQVLSRTVEDSGTVTSVLIPWNSCGAYH
                     TGVLGISTFQYFPYCFLNIINPILSLAYGFTGFRMEKYPPGEVPSDDQVPVLATAGGP
                     AQ"
     gene            3570..>4089
                     /locus_tag="VFV93_00450"
     CDS             3570..>4089
                     /locus_tag="VFV93_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX5163833.1"
                     /translation="MSQAVDKQKTAWHGLSIQDTAKTLGVDPARGLDEAEVAKRVAQY
                     GPNRLAEQKPEPGWHAFMRQYQDMMQIVLLGVAAISIIALQDFATGIFILGLTVFNAI
                     LGLNQEGKAAESVAALRKMLIMKAKVRRNGELVELPAEQLVPGDIVTFEAGDKVPADG
                     RLLTAATLEIEEA"
BASE COUNT          747 a         1299 c         1269 g          774 t
ORIGIN      
        1 gtcatcggct cgaccgtcaa ccccttcgcg accgggatcg cctctggctt cgcggatgtc
       61 tcgatcagtg acggtctctg gatgcgcgtc ctcgtcctgc tcatcgggct cgtgatcggc
      121 gtcttcttcg tgatgcgcta cgcggcccat gtcaaggaag acccctcgaa gtcgctgatt
      181 tacgccatga aggccgagaa cgaggcccgc tggctggccg gtcaggggga ttccgacgac
      241 ttcgggacac tgacagggcg acgcaagctc gtcctcgtct tcttcttcct ggcgttcgcc
      301 gtcatgatct acggtgtcat cccctgggag gacctcggcg tcgggattcc gacgctctgg
      361 tggtggttcc cggagatgac ggcctcgttc atcctgttcg cgatcctgat cgggatgatc
      421 gggcgtctct ccgagaagga cctgaccgac tcgttcgtca acgggtcgcg cgatctgctc
      481 ggcgttgcgc tcatcatcgg catcgcccgc ggcatcaccg tcatcatgaa caacggcatg
      541 atcaccgata ccgtcctgaa ctggaccgag gaagccgtcg ccgacctcgg cggcgccaag
      601 ttcgtcctgc tgttgttcat tctgtttgtc ccactctcat tcctgattcc atcgagttct
      661 ggcctggcga cggttgcgat gccgatcatg gcgccgctgg cgttgttcgc cgggatcaac
      721 ggcgaactcg tcgtgagcgc ctaccagttc ggcagcgggc tggtcgcact cgtcacgccc
      781 acgtcggcgg tcgtcatggg cggcctggcg atcgcgcgag tgggctatgg cacctggttg
      841 aagttcgtct ggccgctact catcgcgctg accgcgctct gcgcgctggc gctcgccatc
      901 ggtgcggcct aggcggtcat gccgcaggcc gcgagggaca cacgggtctg aagggagagt
      961 caactgatga ctgccaccat gtcgatcggc ctgaagggca agagccttct ggacgtcggc
     1021 gaactttcga aggagcaatt cgtcgcgctg ctggacctct ccagcgaact gaaggccgcc
     1081 aagcgcaatg ggaccgaaca gccactgctg aagcagaagg agatcgtgct gatcttcgag
     1141 aagacctcga ctcgcacgcg ctgcgcattc gaggtggccg cctacgacca gggcgcccac
     1201 gtgacctacc tcggacccga gggctcgcag atcggccaca aggaatcgat gaaggacacc
     1261 gcgcgcgtcc tcggccgcat gtacgacgcg atcgagtatc gcggattctc acaggaactt
     1321 gtcgagattc tctcgaccca cgccggcgtg ccggtctaca acggcctgac cgacgagttc
     1381 cacccgaccc agatgctggc cgacatgctg accatgcgcg agcactccgg taagccgctt
     1441 gagcggattg cctacgccta catgggtgac gcacacagca atatgggccg atcgctcctc
     1501 gaaacgggcg cgatcctcgg catggacgtg cggatcatcg cgccccgcga gatctggccg
     1561 gacgaggcgt acatcgcgca acaccggcaa cgcggcgcca catccggcgc gcgaatcacg
     1621 gtcaccgacg atattgcggc cggcgtgaag ggtgtcgact tcatccacac cgatgtctgg
     1681 gtctcgatgg gtgaagccca tgaagtctgg gtcgagcgga tcgagctcct gcggccgtac
     1741 caggtcaaca tggatgttat ccgccagaca ggcaacccgg acgtcaagtt catgcactgc
     1801 ctgccggcgt ttcacaaccg tgagacgaaa gtcggcgagg agatctacga gaagttcgga
     1861 ctcgactgca tggaagtgac cgaagaggtc ttcgagtcac cgcgctcgat cgtcttcgat
     1921 caggcagaga accggatgca cacgatcaag gcgattctcg tcgcgaccct cgctggataa
     1981 ggtcgcgatc gcgcaccgtc gcggcaacgt gtcggtgcgc gatgtgaagg agggcaaccg
     2041 gtggcaagcg acaccagctc aaccagcacc agccgtccac cacgaccgcc aagcatgctc
     2101 gacgcggtca ttccggttgt ggcgctgatc gccctgatct cgttgtcggt ctacctgtac
     2161 ggggtcgatt cgaccaatgg tccgctacag gtctcgatct tcacggctgc ggttgtcgct
     2221 gggctcgtgg cccacaagaa cggctacgcc tacgctgatc tgggcgatgc ggcgatcggc
     2281 ggcatctcaa cggcgatggg cgcgatcttc atcctgctgg cggtcggagc actaatcggc
     2341 acctggaaca tggccggcac gatcccaacc atcgttgact atggcatccg gctgctggac
     2401 ccggcctggt tctatctcgc tgtggcactg atctgtgcca tcaccggtgc cgtgacgggc
     2461 agctcatgga caacggctgg gacgttgggc gtcgcgttcg tcgggatggc gcacatcatc
     2521 ggcgtcaatc cggagatcac ggccggcgcg gtcatctccg gcggctactt tggcgacaag
     2581 atgtcgccgc tctccgagac gacgatcctg acgccgagcc tggttggtgg tctcacgacc
     2641 ggtcagcaca tccgcgcgat ggcatggtca accgggccgg cgttcgccat ctcggcagtg
     2701 ctcttcctta ttatcagcct gaacacggac gtgtcgtcca gcacggctga gatcgacgag
     2761 gcacgagccg tcctgaacga ctcgttcaac atctcgatca tcaacctgct gccggtcgcc
     2821 ctcctcatcg ccttcagcgt gaaaaagttc ccggctttcc tgtccatctt cctgaccgcg
     2881 ctgttctcag gtgtgctggc cggcttcacc cagcgcgacc aggtcattca gtttgcgaac
     2941 gatcccgatc tgagcacgcc attggccatc ctcaaaggca tctacggctc gatggcaacc
     3001 ggctatgtct catcgaccgg caatgaaggc gtcgacatgc tgttctcacg tggcggcatg
     3061 agcagcatgc tctcgacggt ctggatcatc ctcggtgcgc tcgccttcgg ggccatcatg
     3121 gagcacgcgg ggttcctcaa ccggctggtg gaaacgctgc tggagaaagc gacgtcgggc
     3181 ggcgcgctgg tggcgtcggt gatcgggacg gcgattgggc tgaacatcat cgcggccgac
     3241 cagtacatcg cgatcgtcct gccggcccgc tcattccgcg ttgagttccg gcggcgaggc
     3301 tatcagcccc aggtactgtc gcggacggtc gaggattccg gaacggtcac atcggtgctc
     3361 attccctgga acagctgcgg cgcgtaccac accggtgtgc tcggcatctc gacgttccaa
     3421 tactttccct actgcttcct caacatcatc aacccgatcc tctcactcgc gtacggattt
     3481 acgggctttc ggatggagaa gtacccacct ggcgaggtcc cgtcggacga ccaggtaccg
     3541 gtactcgcaa cggcaggagg accagcacaa tgagccaggc agtcgacaag cagaaaacgg
     3601 catggcacgg gctatcaatc caggacaccg ccaagacgct cggagtcgac ccggcgcgag
     3661 gtctcgacga ggccgaggtt gcgaagcggg tcgcgcagta cggaccaaac cggctcgccg
     3721 agcagaaacc tgagccgggc tggcatgcgt tcatgaggca atatcaggat atgatgcaga
     3781 tcgtcctgct cggcgtcgct gcgatcagca tcatcgcgct ccaggacttc gcgaccggca
     3841 tcttcattct cgggttgacc gtgttcaacg cgatcctggg cctgaatcag gaaggcaagg
     3901 cggccgaaag cgtcgcggca ctccgcaaga tgctgatcat gaaagccaag gtgcggcgca
     3961 acggcgaact cgtcgagttg ccagccgagc aactggtgcc cggcgacatc gtcacgttcg
     4021 aggccggcga caaggtgccg gctgatgggc ggctgctgac ggccgccacg ctcgagatcg
     4081 aggaagccg
//
LOCUS       DASUGJ010000016         3725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1668976, whole genome shotgun sequence.
ACCESSION   DASUGJ010000016 DASUGJ010000000
VERSION     DASUGJ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668976"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1386)
                     /locus_tag="VFV93_00455"
     CDS             complement(238..1386)
                     /locus_tag="VFV93_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163834.1"
                     /translation="MDAFARPSTIVVLGVPLRAARGWIAIALVLTAITIDALRPASST
                     WHWYLAALIVTLGAVGSVLLHEIAHVLTARAVGGTVSAIEPAMFGALSDDAYLPRNPR
                     AEAWVAGAGPLASLLLAGGCAVIWRWTFPDESLAAGAAGFLALFNLILFAGNAMPGFP
                     LDGGRIFRAFVWYLTDDLITGTKIAAAYGQAIAVVAFVLGVVLLAVGDTISVWGAWGL
                     IAVWSINRAGREGFLRTIWRETSRGLTIDDVGLGNTRRIEADRSIDDAIDDILQSIGE
                     GPILVRDGDEIVGVITLDQIRRVPRAIWPERTIRDVTLPIDELPRIQYDDELVDLAER
                     FEQTGTQLLIVETRGRITGILERDTTIRRARARVRSIRIEQRRKSKKK"
     gene            complement(1444..1728)
                     /locus_tag="VFV93_00460"
     CDS             complement(1444..1728)
                     /locus_tag="VFV93_00460"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX5163835.1"
                     /translation="MKLIIAVVQNEDADAVVDALLENDFSATRLASTGGFLRRGNTTI
                     MIGVQDEQVDHVLDLIRQEARSRSQAQDSGEVQTAAATVFVLDLEEYQRL"
     gene            complement(1774..2580)
                     /locus_tag="VFV93_00465"
     CDS             complement(1774..2580)
                     /locus_tag="VFV93_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5163836.1"
                     /translation="MTLEHKGRGDPRRSMALLWRGKREPSRGPKPTLAIEQIIAAAVE
                     IADGEGLGAVTMRRLADRLSVGAMTLYTYVPGKAELLDVMLDEVYGETLDSIVSSGNW
                     RERLEAQARRDWELYRRHPWILQISEARSLLGPNEIALYESALESVAGLGLNGREMIS
                     VVSLVTSYVRGAAQVAVDAARVGEQTGVSDEQWWDEPAPILDEYYELTRYPTLSSIDL
                     EGAFDPTTNGVEYHLQLAIDSFEFGLQRVLDGIGAFINQRSVDPTFQPPL"
     gene            2987..>3725
                     /locus_tag="VFV93_00470"
     CDS             2987..>3725
                     /locus_tag="VFV93_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5163837.1"
                     /translation="MNLATVPRAELEMRTRSRSAAVSTASPASAHAITAEGLTKTYKT
                     RSGVVKALDGLDLTVVEGSVLGLLGPNGAGKTTTVRILATLLRPDGGRATVAGYDLAS
                     EADKIRAAIGLSGQYAAVDENLTARENLWMFGRLYQLPKAEARARADELLEQFTLTSD
                     GDRPIKTFSGGMRRRLDLASALIGKPRLLFLDEPTTGLDPRSRLGMWDVIRGLVREGT
                     TLLLTTQYLEEADELADTIAVVDHGRII"
BASE COUNT          670 a         1155 c         1238 g          662 t
ORIGIN      
        1 ggaacgattg aaagcaggca ggagatgacc acgaccaggc aggccgcccg tcgatattgc
       61 aggcgcattg attccccttt ttgtgcgctg tgtctcgcag atcgaccctg ccccgtcagg
      121 gccgacgagc gaatgtgact accccgttag agagctgatt cggcgacgca ggtcaggcgt
      181 tgtctcctgg tcacgagtct accagcaggg gtgtacgtac acaagggggt ggtccggcta
      241 cttcttcttg ctcttgcggc gctgctcgat ccgaatcgag cgaactctgg cgcgcgcgcg
      301 gcggatcgtc gtgtcgcgtt cgaggatacc cgtgatcctg ccgcgcgtct cgacgatcag
      361 caactgggtg cccgtctgct caaaccgctc ggccagatcg accagttcgt cgtcgtattg
      421 tatgcggggc agctcgtcga tcgggagcgt gacatcgcga atggttcgtt ccggccagat
      481 ggcgcgtggg acgcgtcgaa tctggtcgag cgtgatcaca ccgacgatct cgtcgccgtc
      541 tcggacgaga atcggtccct cgccgatact ttggaggatg tcatcgattg cgtcatcgat
      601 cgagcggtct gcctcgatcc ggcgggtgtt gccgaggccg acgtcgtcga tcgtcaagcc
      661 gcgactggtt tcgcgccaga tcgtccgaag gaagccctcc cgcccggccc ggttgatcga
      721 ccagacagcg atcagccccc aggctcccca gactgaaatc gtgtcgccga cagcgagcag
      781 gaccactccc agcacgaagg cgacgacggc gatggcctgg ccgtaggcgg cggcgatctt
      841 cgtcccggta atcagatcgt cggtcaggta ccagacaaag gcacggaaga ttcgcccgcc
      901 gtcgaggggg aagcccggca tggcgttacc ggcgaacagg atcaggttga acagtgccag
      961 gaacccagcc gcgccggccg ccaggctttc gtcgggaaag gtccagcgcc agatcaccgc
     1021 gcagccgcct gccagcaaca ggctcgccag cggtccggcg ccggcaaccc aggcttcggc
     1081 gcgggggttg cgcgggaggt aagcgtcgtc ggagagggcg ccaaacatgg ccggctcgat
     1141 cgcggaaacc gtgccgccga ctgcgcgggc cgtcaggacg tgggcaatct catgaagcag
     1201 caccgaaccg acggcgccga gcgtcacgat caacgcggcg agataccagt gccaggtgct
     1261 gctggccgga cgcagggcgt cgatcgtgat cgccgtcagg acaagcgcga tcgcgatcca
     1321 gccgcgcgcg gcacggagcg gaacgccgag gactacgatc gtggagggac gggcaaaggc
     1381 atccatacag accgttccct ctgccgcgcg acgggatcgt gctggttgtc ggtcggttcg
     1441 cggttagagc cgctggtatt cctcgaggtc gaggacgaag acggttgcgg cggcggtctg
     1501 gacctcgccg ctgtcctgtg cctgcgagcg gctgcgcgct tcctgccgga tcaggtcgag
     1561 gacatggtcg acctgctcgt cctgaacgcc gatcatgatc gtcgtattgc cgcggcggag
     1621 gaagccaccg gtgcttgcca gccgggtcgc gctgaagtcg ttttccagga gagcatcgac
     1681 gacagcgtcg gcgtcctcat tttgcacaac cgcgatgatc agcttcatgg ctcctcattt
     1741 caggacttca ccggcacagg ttgccagaat cgattatagg ggcggctgga aagttgggtc
     1801 aaccgagcgt tggttgataa atgcgccgat gccgtccagc acgcgctgca gcccgaactc
     1861 gaaactgtct atcgcgagct gcaggtgata ttcgacgccg ttcgtggttg ggtcgaatgc
     1921 gccttcgagg tcgatgctcg acagcgtcgg atagcgtgtg agctcgtaat actcgtcgag
     1981 gatcggcgcg ggctcgtccc accactgctc gtcgctgacg ccggtctgct cgccgacgcg
     2041 ggcggcatcg acggcgacct gggctgcgcc ccgcacgtag ctggtcacca gactcacaac
     2101 cgagatcatc tcacgtccgt tcagtcccaa cccggcgacc gattcgagcg cggactcgta
     2161 gagcgcgatt tcgttcggtc cgagcagcga gcgtgcttcg gagatttgca ggatccacgg
     2221 atggcgccga tagagctccc agtcgcgccg ggcctgtgct tcaagtcgct cccgccagtt
     2281 gccgctgctg acgatgctat cgagcgtttc gccgtagacc tcgtcaagca tgacatccag
     2341 gagttcggct ttgccgggca cataggtgta gagcgtcatc gcgccgacgc tgagccggtc
     2401 ggcgaggcgg cgcatcgtga cggctccgag cccctcgcca tctgcgatct cgacggcggc
     2461 ggcgatgatc tgctcgatgg ccagcgtcgg cttcggcccg cgagacggct cgcgcttgcc
     2521 acgccagagc agcgccatgc tgcgtcgtgg gtcgccgcgt cctttgtgct caagcgtcac
     2581 gtccctgggg tctcctcggt cgatcatgaa tctggttggc cacgattcga agtatacgca
     2641 ctggacggcc atgtagcggc acgtgccgcg tacgtacgag gtgtttacaa ctccgtacaa
     2701 cgtacggtat aatcgcgcgg tcgagcattc agatacggtc ggagcgcaga aaggccgtac
     2761 acgatcggtt cggcatcgct gtcgataccc gcacttcagg tcgggaatgc gtactggccg
     2821 gatggacagt gacgtccgac atttgaccga tgttggggac gaccacggcg gctaccatca
     2881 gagaccggcg tcgaggcaga cgccggagca cgggacggtc gccgtcagat caacaggtga
     2941 tgagtgacat cgctgtgctc atcacgccac gacgaaggga tcacgtatga atctagccac
     3001 agtcccacgc gcggaactgg agatgcggac cagatccagg tcggcggcgg tatctaccgc
     3061 ctcgcctgct tccgcacacg cgatcaccgc tgagggcctg acgaagacct acaagacgcg
     3121 ctcgggggtc gtgaaggcgc tagatggact cgatctgacg gtcgtcgaag gcagtgtcct
     3181 cgggctgctg gggccgaacg gggccggcaa gaccacgacg gtgcggattc tggcgacgtt
     3241 gctgcggccg gatggaggtc gagcgaccgt cgccggctac gatctggcga gcgaggctga
     3301 caagatccgg gccgcgatcg gactctccgg ccagtacgcc gccgtcgatg aaaatctgac
     3361 tgcccgcgag aacctctgga tgttcgggcg gctctaccag ctcccgaagg ccgaagcgcg
     3421 ggcgcgagcg gatgagttgc tggagcagtt cacactgacc tccgatggcg accggccgat
     3481 caagacgttc tctggcggca tgcgacgacg actcgacctc gccagcgcgt tgatcggcaa
     3541 accacggcta ctgttcctcg atgagccgac gaccggtctg gatcctcgca gccgcctggg
     3601 gatgtgggat gtcattcgcg gcctcgtccg tgagggaaca acgctgctgt tgacgactca
     3661 gtatctcgaa gaggccgacg aactggccga tacgatcgcc gtcgtcgatc acggccggat
     3721 catcg
//
LOCUS       DASUGJ010000017         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_696633, whole genome shotgun sequence.
ACCESSION   DASUGJ010000017 DASUGJ010000000
VERSION     DASUGJ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696633"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFV93_00475"
     CDS             <1..341
                     /locus_tag="VFV93_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5163838.1"
                     /translation="FRAILAHLLGSEALLAGRATRMLEEDEPLFVSVDPNEIGSDEED
                     TPPTIAGMLEDLTQARHATIARFSALAPAAWQRAGTHPEFGRMTVTQQLAYLVRHEQW
                     HLAELEARGS"
     gene            complement(402..719)
                     /locus_tag="VFV93_00480"
     CDS             complement(402..719)
                     /locus_tag="VFV93_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX5163839.1"
                     /translation="MARFVPVARTDEIAPGSVRQVFVDDDPIAICHVNGEFFAICDIC
                     THDDYYLSGGRLDGYEIECPAHATVFDIRSGEVEIPPAEKPIATYPVRVVDGVIEIGV
                     RDA"
     gene            1013..>3157
                     /locus_tag="VFV93_00485"
     CDS             1013..>3157
                     /locus_tag="VFV93_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163840.1"
                     /translation="MNPLRQCTSRGVLLTIGLLALLAAPLSLPASAQTTDEATQSAAT
                     VAMPQPSPEVTAIWDQTDGALLRGEISGPFVWGEEPLAIATEHYRESTTGLREMVYYD
                     KGRLDILHLPDQPADAWQVVSAALVREMLTGEVQFGAEESVQRGKPPIPVVGDLNQPE
                     ALTYATLAQLAAIDEPAPAPLLPDAKYELEYGAEPSSAVGTPITSLVSARGTVEPDGV
                     TFDDPATAVTVGAYDDVTQRNIAAPFASWVESLPYPDLYLLGHPLTEPYWIDTVVDGV
                     PTRLLFQAFERRVLTYTPGNAEGTRVQSTDVGIHYRLWRNLAQPANFSLVPLSSGIPF
                     GEEIVNAATANLVDPHLLAAISLAASNGDPLATLPNGGKGILGLRPDAAIALGVKPDP
                     QVELSTAAAATGNTAGGAAGAASSTPTATATTTETVPTTTTTEAQQTQVAPTDEASGA
                     AVMPGGIVMAPRWVSDVMADPAHNAAYAAREVARWAPESLDFASIAADYYSGGQPVAD
                     PAAQQAFVDRVVEIRAQLAEQYPPNIMPAMGEVDGQLLGTGHAAYYSPSYDVAWWERT
                     LKLYDSWNVIQPGWVNDPNGYYCVRPGYVPGHRLQLVANGVTVTCTVGDMVADPHLSS
                     WLAHWVIEMNYPLFEALGLDRNNTVEVYHIEDAPVPPVPEPPVEPTPSPTPEPSPTPE
                     PSPTPSPTATPQPSPSPTPSPTPAPTGTPAPSP"
BASE COUNT          553 a         1111 c         1025 g          468 t
ORIGIN      
        1 cgttccgggc gatcctggcc cacctgctcg gctcggaagc gttactggcc ggccgggcta
       61 cccgaatgct tgaggaggac gagccgctgt tcgtctcggt cgatccgaac gagatcggca
      121 gcgacgagga ggacacgccg ccaaccatcg ccgggatgct ggaggacctg acgcaggcgc
      181 gccacgcaac gatcgcccgc ttcagcgcgc tcgcccccgc cgcctggcaa cgggccggca
      241 cccaccccga attcgggcgg atgacggtga cccagcagct cgcctacctc gtacgccacg
      301 agcaatggca tttggcggag ctggaagcgc gggggtcgta acgcgtgacg cgtgatgcgt
      361 gacgcgcagg tgcgaacctc atccgcgtca cgcgttacgc gttacgcgtc acgaacccca
      421 atctcgatca ccccatccac cacccgcacc gggtaggtcg cgattggttt ctcggcgggg
      481 ggtatctcga cctcgccgct gcggatgtcg aagacggtgg cgtgggcggg gcattcgatc
      541 tcatagccgt cgaggcgacc gccggagaga tagtagtcat cgtgggtgca gatatcgcag
      601 atggcgaaga actcgccgtt gacgtggcat atcgcgatcg ggtcatcatc gacgaatacc
      661 tggcgcaccg agccgggcgc gatctcatcg gtccgggcga ccgggacgaa gcgtgccatg
      721 caacctgttc cttgcatcgt tcgttgtcag taatacggcc ggcgggagcc gccgctcggg
      781 tctaaggata cggcacgcgg cttgctgaaa cgcggccgga cgtgagcctg gccttgccag
      841 actgtcatgg gatgacagtc ggcgatgttg gtcagcacat caaacgcggt tacaatggtg
      901 atcccgttgt atggggtccg cgtattggat gtcaacgttc aatgaatgaa tgcaccgggg
      961 aacccgtgtg gccgctgggg tagcacaggc cacgcggagg gaaggaaagc tgttgaatcc
     1021 tctgcgacag tgtacgtcgc gcggcgtact gctgacgatc ggactactcg ccctgctggc
     1081 cgcgccgctc agcctgcctg ccagcgccca gacgactgac gaagccaccc agagcgcagc
     1141 aacggttgcc atgccgcaac cgagtcccga agtcaccgcc atctgggatc agaccgatgg
     1201 cgcgctgctg cgtggggaga tctccggccc gttcgtctgg ggcgaggagc cgctcgcgat
     1261 cgcgaccgag cactaccgcg agagcacgac cggcctgcgc gagatggtct actacgacaa
     1321 aggccggctc gacatcctgc atctgcccga tcagccggcc gatgcctggc aggtcgtcag
     1381 cgccgcgctc gttcgcgaga tgctgaccgg cgaggtccag ttcggcgccg aggagagtgt
     1441 ccagcgcggc aagccaccga tcccggtcgt tggcgacctg aaccagccgg aggcgctgac
     1501 ctatgcgacg ctcgctcaac tggccgcgat cgacgaacca gctccggccc cgctcctgcc
     1561 ggatgcgaag tacgagcttg agtacggcgc cgagccgagc agcgctgttg ggacgccgat
     1621 cacgtcactc gtcagcgccc gtggcacggt cgagccggac ggcgtgacgt tcgatgatcc
     1681 ggcaacggcc gtcaccgttg gcgcctacga cgatgtcacc cagcgcaaca tcgccgcgcc
     1741 gttcgccagc tgggtcgaga gcctgccgta tcccgacctc tacctgctcg gccacccgct
     1801 gaccgagccg tactggatcg acacggtcgt cgatggcgtg ccgacacggc tgctgttcca
     1861 ggccttcgag cgccgcgtcc tgacctacac gccgggtaac gccgagggga cgcgggtcca
     1921 gtctaccgac gtcgggatcc actaccggct ctggcgcaac ctggcccagc cggcgaattt
     1981 ctcgctcgtc ccgctctcca gcggcatccc gttcggcgag gagatcgtca acgccgcaac
     2041 cgccaacctg gttgatccgc acctgctggc cgccatctcg ctggccgcct cgaacggcga
     2101 tccgctggcg acgctgccga acggcggcaa gggtatcctc gggctgcggc cggacgccgc
     2161 catcgcgctc ggcgtcaagc ccgacccgca ggtcgagctc tcgacggctg cggctgccac
     2221 cggcaatacc gccggcggcg cggccggggc ggcatcgtca accccaaccg caaccgcgac
     2281 aacgacagaa accgtcccaa caacgacaac aaccgaggcg cagcagacgc aggtcgcccc
     2341 aacggacgaa gcctccggcg cggccgtcat gccgggcggg atcgtgatgg cgccgcgctg
     2401 ggtcagcgat gtgatggctg acccagccca caacgccgcc tacgccgccc gcgaggtggc
     2461 acgctgggcg ccggaatcac tcgacttcgc cagcatcgcc gccgactact acagcggcgg
     2521 tcagccagtc gccgatccgg ccgcccagca ggcgttcgtc gatcgggtgg ttgagatccg
     2581 cgcccagctg gccgagcagt atccgccgaa tatcatgccg gccatgggcg aggtcgacgg
     2641 ccagctgctc ggaaccggtc acgcagccta ctactcgccc agctacgatg tcgcctggtg
     2701 ggagcggacg ctcaagctct acgatagctg gaatgtgatc cagcccggct gggtgaacga
     2761 cccgaacggc tactactgcg tccgccccgg ctacgtgccc ggccaccggc tgcaactggt
     2821 cgccaacggc gtcaccgtca cctgcacggt gggcgacatg gtcgccgacc cgcacctgtc
     2881 gtcctggctg gcgcactggg tgatcgagat gaactacccg ctcttcgagg cgctcgggct
     2941 ggaccgcaac aacacggtcg aggtctatca catcgaggac gcaccggttc cgccggtgcc
     3001 ggagccgccg gtcgagccga caccatcacc aacaccggag ccgtcaccga cgccggagcc
     3061 gagcccaacg ccaagcccga cggcaacgcc gcagccgagt ccgtcaccga cgccatcgcc
     3121 gacgccggcc ccaaccggca caccggctcc gtcgccg
//
LOCUS       DASUGJ010000018         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_974973, whole genome shotgun sequence.
ACCESSION   DASUGJ010000018 DASUGJ010000000
VERSION     DASUGJ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974973"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFV93_00490"
     CDS             <1..527
                     /locus_tag="VFV93_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163841.1"
                     /translation="KWRLVWLRDTHPDAQIETELVQHQNQMAVFRARVTIPSGASATG
                     WGSEGADDFGDYLEKAETKALGRALAALGFGTQFCPDYDFGAERQRVVDSPVDIRSGR
                     TSGSANGSRQEATPRQVKYLYAVAREVGLSNDELEERSQEGFGCTISELSRRDISTLI
                     EQIQAGRPVHSIAS"
     gene            566..775
                     /locus_tag="VFV93_00495"
     CDS             566..775
                     /locus_tag="VFV93_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163842.1"
                     /translation="MVALQAQPVVVLDDRESMKVEMKRLLFRLEDGYEKIAQAAVNGD
                     DVRRWEDVWLQLLCEYEELYDRMAA"
     gene            913..2097
                     /locus_tag="VFV93_00500"
     CDS             913..2097
                     /locus_tag="VFV93_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5163843.1"
                     /translation="MSAGYDLERARANLPVLRDWTYLNTGTVGVMAEPVLQKHLEQIA
                     DHERFGHATQARAIEGYERARRTLAMLTGSNPSDIALNRNATDGINWVAATFPLGDGD
                     EVVTSTEEHPAMVYPWLAACERAGARLRFIDLHTSPDALRSALETVVSPATRVVALSQ
                     VSCETGKRVPVEIVRDVVGPEVAILVDASQSVGQFPIDIPALRANFVIGNGHKWLAGP
                     KGTGFAWFDADSLDLVPPVYFGDGAVDPRWSRSYYQSDPSPRLHFGGNASRFEYGTRA
                     WHMYEGLADAIEYQAGFGWDAIFDHVDRMSSLAKAVLEEIPGVDVITPATWQESSGIV
                     SFTIMGMAGEDISRELWENERITQRRVEVPSAVRVSCTYFTSEEDVTRLGQAVDRIAR
                     GR"
     gene            complement(2033..>2822)
                     /locus_tag="VFV93_00505"
     CDS             complement(2033..>2822)
                     /locus_tag="VFV93_00505"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5163844.1"
                     /translation="RSRSSRSVHSSIATGRPVGRPFVWKWQVVVTTTLWLVRHGETSS
                     NADGVFQGHLDVGLNQRGEEQAAAVGERLACIDFDAVYASDLERAARTAEIIVNGRRP
                     VVLDPDLREMHYGVLQGVRYHDAPSVLASHGMSEAWNSGEFHRKGLASPGGESLRQFR
                     HRSRRFLDRVDRLHPPDAGHDVLIVAHGGKLSVLMTVLLEIPSISRRSFRFANCGITR
                     VSRSGLRTTLDLHNLVVWDDTLLVSDHAQSGRLLDPGESHLPRT"
BASE COUNT          517 a          918 c          904 g          483 t
ORIGIN      
        1 tcaaatggcg tcttgtctgg ctgcgggaca cgcatcccga cgcccagatc gagaccgaac
       61 tcgtccagca tcagaatcag atggcagtct tccgcgccag agtcacgatc ccgagcggcg
      121 ccagcgcgac cggctggggc agcgagggcg ccgacgactt cggtgactac cttgagaagg
      181 ccgagacgaa ggcgctcggc cgcgctctgg ccgcgttggg cttcggcacg cagttctgcc
      241 cggattacga cttcggcgcc gaacggcagc gcgtggtcga ttcgccggtc gatatccgca
      301 gcggccggac atctggttcc gccaatggct cccgtcagga agccacgccc cgccaggtga
      361 agtacctcta cgcggtcgcg cgggaggttg ggttgtcgaa cgacgaactc gaagagcggt
      421 cgcaggaggg tttcggctgc acgatctcgg agctgagccg ccgcgacatc tcgacactga
      481 tcgagcagat tcaggccggc cggccggtcc acagcatcgc ttcctgagag acgcagtcgc
      541 ctgattcggg caggaggaac cgttaatggt cgcacttcag gcacaaccag tcgtggttct
      601 ggacgatcgc gagtcgatga aggtcgagat gaagcgtctg ctcttcaggc tcgaagatgg
      661 ctatgagaag atcgcccagg cagccgtcaa cggcgacgat gtccgccgct gggaagacgt
      721 ctggctccag cttctctgtg agtacgagga actctacgac cgaatggcgg cgtgacaacc
      781 cgtcacaatc tactctgcac gagcactcag cggccgggtc caaaccccgg ccgctttcgc
      841 atctgttgac gcgtctcgtc agcactgtta ccgtccgcac tcgaacggac atcgcaactg
      901 atggagacaa cgatgagtgc tggatacgac ctggaacgag cacgagcgaa cctgccggta
      961 ctgcgcgact ggacctacct caacacgggc acggtcggcg tcatggccga accggtcctg
     1021 cagaagcacc ttgagcagat cgccgaccac gagcgcttcg gtcatgcgac ccaggcgcgg
     1081 gcaatcgagg gttacgagcg cgcccgtcgc acactcgcga tgctgaccgg ttccaacccc
     1141 tccgacatcg ctctcaatcg caacgcgacc gatggcatca actgggtcgc cgccaccttc
     1201 ccactcggcg acggcgatga ggtcgtcaca tcgactgaag agcatccggc catggtctat
     1261 ccgtggctgg ccgcctgcga gcgggctggc gcgcgcctgc gcttcatcga cctccacacc
     1321 agtccggacg cgctacgatc cgcgctggag acagttgtct cgccggcgac gcgtgtcgtc
     1381 gccctcagtc aggtctcgtg tgagacgggc aagcgcgtcc cggtcgagat cgtccgtgac
     1441 gtcgtcggcc cggaagtggc gatcctcgtc gacgcctcgc aatcggtcgg tcagtttccg
     1501 atcgacattc ccgcgttacg tgccaacttc gtgatcggca acggccacaa gtggctggcg
     1561 gggccgaagg gaaccggctt cgcctggttc gacgcggatt cgctcgacct cgtcccaccg
     1621 gtctacttcg gcgatggcgc cgtcgatccg cgctggtcgc gtagctacta ccagtccgat
     1681 ccgtcgcccc gtctgcactt cggcggcaat gcgagccgct tcgagtacgg cacccgcgcc
     1741 tggcacatgt acgaggggct ggccgacgcg atcgagtatc aggccggctt cggctgggac
     1801 gccatcttcg accatgtcga tcggatgtcg tcgctggcga aggccgtact ggaggagatc
     1861 cccggcgtcg atgtcatcac gcccgcgacg tggcaggaat cgtccggcat tgtctcgttc
     1921 acaatcatgg ggatggccgg cgaggatatc agccgcgagc tttgggagaa tgagcgcatc
     1981 acccagcgac gggttgaggt gccgagcgcc gtccgcgtct cgtgcacgta ctttacgtcc
     2041 gaggaagatg tgactcgcct gggtcaagca gtcgaccgga ttgcgcgtgg tcgctgacca
     2101 gcagggtatc gtcccagacg acgagattgt gcaggtcgag cgtcgtccgc aggccggatc
     2161 gtgacacacg cgtgatgccg caattcgcga agcggaacga gcggcggctg atcgagggaa
     2221 tctccagcag caccgtcatc aggacactca acttgccgcc gtgggcgacg atgaggacat
     2281 catgcccggc atcgggcggg tggagccggt cgacgcgatc gaggaaccga cgcgagcgat
     2341 gccggaactg gcgcaggctc tcgccgcccg gcgatgccag ccccttgcgg tgaaattcgc
     2401 cgctgttcca cgcctcgctc atgccgtgcg atgccagaac cgacggcgca tcgtggtagc
     2461 gaacaccctg caagacgcca tagtgcatct cacgcaggtc ggggtcgagc acgacgggac
     2521 ggcgaccgtt gacgatgatc tcggctgttc gggcggcccg ctcaaggtcg ctggcataga
     2581 cggcgtcgaa gtcgatgcaa gccagccgtt cgccgacggc cgctgcctgc tcctccccac
     2641 gctggttcag accaacgtcg agatgaccct gaaagactcc gtccgcgttc gagctcgtct
     2701 cgccatgccg gacgagccag agtgttgtcg tcaccacgac ctgccatttc cagacgaaag
     2761 ggcggccaac cggccgcccc gttgcgatcg acgaatgaac ggacctactt gaccgtgatc
     2821 tc
//
LOCUS       DASUGJ010000019         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1947521, whole genome shotgun sequence.
ACCESSION   DASUGJ010000019 DASUGJ010000000
VERSION     DASUGJ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947521"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1695
                     /locus_tag="VFV93_00510"
     CDS             166..1695
                     /locus_tag="VFV93_00510"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX5163845.1"
                     /translation="MTPFSNLSLRWKLLGSFALVLIILAGMSIAAYRTTVSNEHADDA
                     ESQTLETIDETNATYAALLDMESGYRGFLLTGQDRFLDPYFNGKRDFENLLSMLMQRS
                     QDSSSELARWRDIAQRAETWQQEIIEPGIQIRRDVNAGAAEIDAVTNWVSTNAGRQQV
                     DDMREIFAEAIDAERAVLDERQQEAADENARLRQTLIFGTLLAVVLGVGMALLLARDV
                     VGGVGRLARSAERIAGGDLTERTGLRRNDEVGDAAAAFDRMADQLQRSVIERDQREQD
                     LARSNAELEQFAYVASHDLQEPLRAVVSYVQLLEKRYKDQLDERASKYIAYAVDGGQR
                     MQTLINDLLTYSRVGRANEEFEPVDMEHVLDRAITSVRVAVEESEAVITHDPLPVVTA
                     DERMLTQLFQNLLSNAIKFSGDRPPRVHVSAARQPDEWLFAVRDEGIGIAPEYAQRIF
                     VIFQRLHGRDEYPGTGIGLAICKKIVDRHGGRLWVESTFGAGATFYFTLLDPDPGDVT
                     A"
     gene            1692..2153
                     /locus_tag="VFV93_00515"
     CDS             1692..2153
                     /locus_tag="VFV93_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5163846.1"
                     /translation="MTITYPSARAEPIDILLVEDSPADVDLTREALEDAKVRNNLHVV
                     GDGVDALAFLRREDPYSDAPRPDLILLDLNLPRKDGREVLAEIKADPALLRIPVVILT
                     TSAAEQDILRSYDLHANCYVTKPVDLEQFITVIKSIENFWLMIVKLPDGLT"
     gene            2164..>2669
                     /locus_tag="VFV93_00520"
     CDS             2164..>2669
                     /locus_tag="VFV93_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5163847.1"
                     /translation="MTDGADQPLKILLIEDNPADADLIRDVLDDLDATAEPGAPTFAL
                     AHADRLSRAQERLADGDIELVLLDLTLPDSHGFETFSTVASRFPDLPIVVLSGLDDET
                     LAMRAVRDGAQDYLVKGRVDADLLGRTLRYSIERRRAASERERLRQEQTRIDTALRAR
                     NELLASIS"
BASE COUNT          499 a          866 c          859 g          445 t
ORIGIN      
        1 ccccctgtcg tcctgagccg cagcgaagga cctctccgcg ttaacatcgt gactggtcaa
       61 agcggagagg tccttcgctg cggctcagga cgacaggggg gcgttcgtgt atgctcccca
      121 ttcggggcga acactgcacc acgcgcggat gggacactgg ccgacgtgac gccattctca
      181 aatctgagcc tgcgatggaa gctgcttggc agcttcgcgc tggtgctgat cattctggcc
      241 ggaatgagca tcgccgccta ccggacgacc gtctcaaatg agcatgccga cgacgccgaa
      301 tcgcagacgc tggagaccat cgacgagacc aacgcgacct acgccgccct gctggacatg
      361 gagtccggct atcgtggctt cctgctgacc ggccaggatc gcttcctcga tccgtatttc
      421 aatggaaagc gtgatttcga gaacctgctg agtatgctca tgcaacgctc acaggattca
      481 tcgtctgagc tggcccgttg gcgggacatc gcgcagcggg ccgagacctg gcagcaggag
      541 atcatcgagc cggggatcca gatccggcgc gacgtcaacg ccggggcggc cgaaatcgac
      601 gccgtaacca actgggtcag cacgaatgcc ggccggcagc aggtcgatga catgcgcgag
      661 atcttcgcgg aggcgatcga cgccgagcgg gccgtcctgg acgagcgcca gcaggaagcc
      721 gccgatgaga acgcgcgcct gcgacaaacg ctgatcttcg ggacgttgct ggcggttgtc
      781 ctcggcgttg ggatggcgct gctgctggcc cgcgatgtcg tgggtggagt tggccggctg
      841 gcccggtctg ctgaacgaat cgccggaggc gatctgaccg aacgaaccgg attgcgccgg
      901 aacgatgagg ttggtgacgc ggcggcggcc ttcgaccgga tggcagacca gctccagcgg
      961 tcagtcatcg aacgtgacca gcgcgagcag gacctggccc gctcgaacgc ggagctcgaa
     1021 cagttcgcct atgtcgcctc gcacgacctg caggagccgt tgcgagcggt cgtcagctat
     1081 gtgcaactct tggagaagcg ttacaaggac cagctcgacg aacgcgccag caagtacatc
     1141 gcctacgcgg tcgatggtgg ccagcgcatg cagacgctga tcaacgacct gctcacctat
     1201 tcgcgtgtcg gacgggcgaa tgaggaattc gagccggtcg atatggaaca cgtgctcgac
     1261 cgggcgatca ccagtgttcg tgtcgccgtt gaggagagcg aggccgtcat cacccacgat
     1321 ccgctgccgg tcgtgacggc cgacgagcgc atgttgacgc agctctttca gaacctgctc
     1381 agcaacgcca tcaagttcag cggggatcgc ccgccgcgcg tgcatgtctc ggcagcacgg
     1441 cagcccgatg agtggctctt cgccgttcgc gacgaaggga tcgggatcgc gccggagtac
     1501 gcgcagcgga tctttgtgat ctttcagcgc ttgcatggcc gcgatgaata ccccggcacc
     1561 ggcatcgggc tggcgatctg caagaagatc gttgatcgac acggtggccg gctctgggta
     1621 gaatcgacgt tcggcgcagg cgcgaccttt tacttcaccc tgctcgatcc agatcctgga
     1681 gacgtcaccg catgacgatt acgtatccca gcgcgcgcgc agagccgatc gatatcctgc
     1741 tggtcgagga cagcccggct gatgtcgacc tgacgcgcga ggcgctggaa gacgcgaagg
     1801 tccgcaacaa cctgcatgtc gtcggcgatg gtgtcgatgc gctggcattc ctgcgccggg
     1861 aagacccgta cagcgatgcg ccccgaccgg acctgatcct gctcgacctc aacctgcccc
     1921 gcaaggacgg ccgcgaggtc ctggccgaga tcaaggccga cccggcgctg ctgcggatcc
     1981 cggtcgtcat cctgacgaca tcggcagccg agcaggatat cctgcgcagt tacgacctgc
     2041 acgccaactg ctacgtcacc aagccggtcg atcttgagca attcatcacc gtcatcaagt
     2101 cgatcgagaa cttctggctg atgatcgtca agctgcccga cggtctgacc taggctgcca
     2161 acgatgaccg acggggcaga ccagccgctg aagatcctgc tgatcgagga caacccagcc
     2221 gatgcggacc tgatccgcga cgtgctcgac gatctcgacg ccaccgccga gccgggcgcg
     2281 ccgaccttcg cgctcgccca cgccgaccgg ctctcccgcg cgcaggaacg gctcgccgac
     2341 ggcgacatcg aactggtact gctggacctg acgctgccgg acagccacgg cttcgagacc
     2401 ttcagcacgg tcgccagccg cttcccggac cttccgatcg tcgtcctcag cggtctcgat
     2461 gacgaaacgc tggcgatgcg cgccgtccgt gacggtgccc aggattacct cgtcaaaggg
     2521 cgggtcgatg ccgacctgct cggccggacg ctgcgctact cgatcgagcg ccgccgggcg
     2581 gccagcgaac gggagcggct ccggcaggaa cagacgcgga tcgataccgc gttgcgcgcc
     2641 cgcaatgagc tgctggcgtc gatctccca
//
LOCUS       DASUGJ010000020         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1530760, whole genome shotgun sequence.
ACCESSION   DASUGJ010000020 DASUGJ010000000
VERSION     DASUGJ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530760"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..2181
                     /locus_tag="VFV93_00525"
     CDS             145..2181
                     /locus_tag="VFV93_00525"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5163848.1"
                     /translation="MSSTVAERQAEIEPDEARERRRPVDTSSDQPGWLDRYSMAVATA
                     LTALFLLAGWLLGRLGLIGENAQVALYGAAYVTGGTRAAWTALRSLWRRHIDVDLLMV
                     LAAIGAAIIGSWLEGGILLFLFSLGNTREHYAMGRTHSAIRALMDLRPEEALVLRDGA
                     ERVVLVEDLVLDDTVIVRPGERIPADGDILRGQSEIDQSAITGESMPVARGAGESVFA
                     GTINGRGVLHLRVTRLANESVLAKIIDIVDKAQAEKSATQRFTDRFEGLYAGGVIAAA
                     ALYAVIPALFFGRDANDAFYQAMILLVVASPCALVISTPASTLSALANAARNGILFKG
                     AAHLEDLGVARVIAFDKTGTLTAGRPRLTDVVPIGDVTADALLALTAAIERHSEHSLA
                     SAVVEAAETRNLSLVTAGAVEAVVGRGIQATVDGALILIGNDTLFAEHGHPLDQRVTS
                     LLNQFRDDGKSTMIVGRAAEEGVEVLGVLAVADTVRPAARTAIEQLRELGIEKTLMLT
                     GDNQRAAGAIARQTGIAEVRSDLLPAEKLDVIAELQHQHGVVVMVGDGVNDAPALAAA
                     SIGVAMGAAGTDVALETADVVLMADDLTRLPYAVALSRRTRRIIRQNLTFALAVITIL
                     VSGTLLGITTLSLGVVGHEGSTIVVVLNGLRLLGGVPSVKRERQLRPVTAGSRA"
     gene            2215..2775
                     /locus_tag="VFV93_00530"
     CDS             2215..2775
                     /locus_tag="VFV93_00530"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5163849.1"
                     /translation="MNDTTDTGLVSVEEYLRIEEVSPIKHESVSGVLYAMMGGTKAHS
                     EILGNIYAAIRPEARRNRCRIFPGDVKAQVDAERIYYPDLIVACDPDDRDPLITRNPC
                     LVVEILSPSTQSIDRREKASTYRTIASLRSYLIVDQDEMRVEHYYRNDDDAWTLEFVT
                     EGAVTLRCPPMELWLDDIYEELPQAE"
BASE COUNT          522 a          932 c          941 g          516 t
ORIGIN      
        1 aacaccttcc caagttgaca cgctattgtg atgctctgag tcagccattg acgggatctc
       61 tacgccttgt gcggtatgat aggtgcgaat gattcgcagg tgcgaatgat tctcgatatt
      121 gaccatgagc gtgagggaga agccgtgagc agcacagttg cagaacggca ggcggagatc
      181 gagcctgacg aagcacgcga gcgtcgccgg ccggtggata ccagcagcga tcaacctggc
      241 tggctcgacc ggtactcaat ggcggttgcc accgcgctga cggcgctctt tctgctggcg
      301 ggctggttgc tggggcggct gggcctgatc ggcgagaatg cccaggtcgc gctgtatggc
      361 gccgcctatg tcactggcgg gacacgggca gcctggacag cgctccggtc gctctggcga
      421 cggcatattg acgtcgatct gctgatggtg ctggccgcga tcggcgcggc gatcatcggc
      481 agctggctgg agggcggcat cctgctgttc ctcttctcgc tcggcaacac gcgtgaacac
      541 tacgcgatgg gccgcaccca cagcgcgatc cgtgcgctga tggacctgcg gccagaagag
      601 gcgcttgtcc tgcgtgatgg cgctgagcgt gtcgtgctgg tcgaggatct ggtgctggat
      661 gacaccgtca tcgtccgtcc cggtgagcgg attccggccg atggcgacat tctgcgtggt
      721 cagtcggaga tcgaccagtc ggccatcacc ggcgagtcga tgccggtcgc ccgtggggcc
      781 ggcgagagcg tcttcgctgg aacgatcaac gggcgcggcg tccttcacct gcgggtgacg
      841 cggctggcga acgagagcgt gctggcgaag atcatcgaca tcgtcgataa ggcacaagcc
      901 gagaagtcgg ctacccagcg cttcaccgat cgcttcgagg ggctctatgc aggtggcgtg
      961 atcgctgccg ccgcgctcta tgctgtgatt cccgcactgt tcttcggccg cgacgccaac
     1021 gacgcgttct atcaggcgat gatcctgctg gtcgtcgcct cgccctgcgc gctggtcatc
     1081 tcgacgccgg cctcaacact gtcggcactc gccaacgcgg cccgcaacgg catcctcttc
     1141 aaaggcgctg ctcacctcga agacctcggt gttgccaggg tgatcgcgtt cgacaagact
     1201 ggcacgctga ccgccggccg gccacgactg accgatgtcg tgccgatcgg cgacgtcacc
     1261 gctgatgcgt tgctggcact aacggcggcg atcgagcgac actcggagca ctcgctggcc
     1321 agcgcagtcg tcgaggcggc cgagacccgc aacctgtcgc tcgtgactgc cggagcagtc
     1381 gaggcggtcg tcggacgcgg catccaggcg acggttgatg gtgcgctgat cctgatcggc
     1441 aacgacacac tcttcgccga gcacggccat ccgctcgatc agcgcgtcac gtcgctgctc
     1501 aaccagttcc gcgacgacgg caagtcgacg atgatcgtcg gccgggcagc tgaggagggc
     1561 gtcgaggtgc tgggcgtcct cgccgtcgcc gacaccgtcc ggccggctgc ccgcacggca
     1621 atcgagcagc tccgcgagct cggcatcgag aagacgctga tgctgaccgg cgacaaccag
     1681 cgcgcggcgg gcgcgatcgc ccgccagacc ggtatcgccg aggttcgcag cgacctgctg
     1741 ccggccgaaa agctggacgt catcgccgag ctgcagcatc agcacggcgt cgtcgtgatg
     1801 gtcggcgacg gcgtcaacga cgcaccggcc ctggcggcag cctcgatcgg cgtcgcgatg
     1861 ggagccgccg gaaccgatgt cgccctcgaa acggccgacg tcgtcctgat ggccgacgac
     1921 ctgacgcgcc tgccctacgc ggtcgcactc tcgcggcgga cccgccgaat cattcgccag
     1981 aatctcacct tcgcgctggc cgtcatcacg attctcgtca gcggaacgct gctgggtatc
     2041 acgacactct cgctcggcgt cgtcggccac gaaggcagca caatcgtcgt cgtcctcaac
     2101 ggcctgcgcc tgcttggcgg cgtcccgagc gtcaagcgcg aacgacagtt gcggcccgtc
     2161 acggctggaa gccgggcgtg atcggcgact ccacgctagc cgtacaatgg gcagatgaac
     2221 gacacaacgg acacgggcct tgtcagcgtc gaagagtatc tccgcatcga ggaggtcagc
     2281 ccgatcaagc atgagtccgt cagtggcgtc ctctacgcga tgatgggtgg tacgaaggcg
     2341 cacagcgaga tactgggcaa tatctacgca gctatccgcc ccgaagcacg tcgcaatcga
     2401 tgtcggattt tcccaggcga cgtgaaagca caggtcgatg ccgaacggat ctactatccg
     2461 gatctgatcg tcgcctgcga tccggatgac cgggacccgc tgattacccg caatccctgt
     2521 ctggtcgttg agattctctc cccgagcacc cagtcgatcg atcggcgcga gaaggccagc
     2581 acctacagaa cgatcgccag cttgcgctcg tacctcatcg tcgatcagga cgagatgcga
     2641 gtcgagcact actaccgcaa tgacgacgat gcatggactc tcgagttcgt gaccgaaggc
     2701 gccgtgacac ttcgttgtcc acctatggaa ctatggttgg acgatattta cgaggaactc
     2761 ccgcaagctg agtagtccac cactctggtc ctgacgagtt gatggcaagg cgaagatgca
     2821 cgaggtccct gtcgtcctga gccgcagcga aggacctccc cgtgttaacc ccgtgactgg
     2881 tcaaagcgga gaggtccttc gctgcggctc a
//
LOCUS       DASUGJ010000021         4159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_696960, whole genome shotgun sequence.
ACCESSION   DASUGJ010000021 DASUGJ010000000
VERSION     DASUGJ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696960"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /gene="recQ"
                     /locus_tag="VFV93_00535"
     CDS             complement(<1..1520)
                     /gene="recQ"
                     /locus_tag="VFV93_00535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX5163850.1"
                     /translation="MQTTPRQPARAGDPAGEHPTAEQIAATLQSTFGYRTFRPLQEEI
                     VNAILDRQDVFVLMPTGGGKSLCYQLPALLLDGLTVVVSPLIALMKDQVDALQALGVP
                     ATFINSSLDAGEAGERQAAVARGDIKLLYVAPERLVLPGFLHLLQHRGVTMFAIDEAH
                     CVSEWGHDFRPEYRELRQLRDRFPEAIFTAFTATATPRVQQDIRDQLGLERARSFQGS
                     FNRPNLYYEVRPKQAAYGQLLEYVHSRKGASGIVYCQTRASTEDLADRLRGDGINAAA
                     YHGGMDGKQRQRVQEAFVRDNVQIIVATIAFGMGIDKPDVRFVVHYDLPKNLEGYYQE
                     SGRAGRDGDPADCILFFTYGDVGKHLHFIDDKPTDALREAARQQLRQMTDWAESTTCR
                     RKALLAYFDEEWTGVQDPCCDVCRNPVDEVDYTVAAQMFLSCVKRTGERFGAAYVIDV
                     LRGSTAERIMRFGHNNLSTWGIGKDRSKEEWNYIARGIVRAGFARQAAEDYNSVKIT"
     gene            1662..2270
                     /locus_tag="VFV93_00540"
     CDS             1662..2270
                     /locus_tag="VFV93_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX5163851.1"
                     /translation="MPRGDTALNHLRAEAASCTRCPLHEIGTQTVFGAGPASARLMFV
                     GEAPGAQEDATGQPFVGPAGKVFDEAIAAAGIDRASVYVTNAVKHRPWKRSESGRQKN
                     RAPKQSEIKACAIWLDGEIEIIQPQLICCLGAVAAKRLLGKDFRLMEQRGQWFSSDDG
                     RDVLATVHPSFVQIQRPEARERWMSTFVDDLRAVKQRLDDAH"
     gene            2381..3382
                     /locus_tag="VFV93_00545"
     CDS             2381..3382
                     /locus_tag="VFV93_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HEX5163852.1"
                     /translation="MLALTAAEIRDLVPMRDAIELMKSVFAANSRGETISPLRTPINM
                     PDGSGVVLFMPAYVPPSSTAPAAAGSKIVSVFAGNRDLGLPTINAIVIMVDPTTGQPL
                     GLLEGATITALRTGAVSGAATDLLARPESTTLAIIGAGAQGVTQAAAVSAVRDIREIR
                     VSDLSEAARANFAERLAVWAPAMAGKVRPAESAQEAVEGVDIICTATTATRAVFEDAW
                     LKPGTHINGIGAYTPQMQEIPDATVGRARIVVDAVEAVLHEAGDLIQPIERGVLTEAD
                     IQVELGHVVDGVAVGREDNEQITFFKSVGNAIQDMIVGAAALKAAGERGVGQQLALE"
     gene            complement(3471..>4159)
                     /locus_tag="VFV93_00550"
     CDS             complement(3471..>4159)
                     /locus_tag="VFV93_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5163853.1"
                     /translation="NTESIANVALVIKNGGDWYASMGTEKSKGTKLITFQGPLKRLGV
                     VEVEMGANLKDLIFNVYGGMKDGYEFKGVQTGGVSAGPLTEDQLDVPVDFDSLTPLGG
                     MLGSGGFVVFDKSVCAVEFARYLQAFNRYESCSKCTPCRLGNPALVEIVDRLRFGTGR
                     PTDLMLIEQASKHVIELSLCGLGQVAPMPLLGMLKTYRDEFEAHLNGVCPTGNCPMHS
                     HVDMALAADG"
BASE COUNT          737 a         1328 c         1373 g          721 t
ORIGIN      
        1 gtgatcttga ccgagttgta gtcctcggcg gcctggcggg caaaaccggc ccggacgatg
       61 ccgcgcgcga tgtaattcca ctcttccttg ctgcggtcct tgccgatccc ccaggtcgag
      121 aggttgttgt gcccgaagcg catgatgcgc tcggcggtcg agccgcgcag gacatcgatg
      181 acgtaggcag cgccgaaccg ctcgcctgtc cgcttgacac aggacaggaa catctgggcc
      241 gcgacggtgt agtcgacctc gtcgaccggg ttgcgacaga catcgcagca agggtcctgg
      301 acgccggtcc attcttcgtc gaagtaggcc agcaacgcct tccggcgaca ggtcgtgctc
      361 tcggcccagt cggtcatttg gcgcagctgc tgtcgggcgg cttcgcggag cgcgtcggtc
      421 ggcttgtcgt cgatgaaatg caggtgcttg ccgacatcgc cgtacgtgaa gaacaggatg
      481 cagtcggccg ggtcgccgtc gcggccggcc cggccgctct cctggtagta gccctccagg
      541 ttcttgggca ggtcatagtg gacgacgaag cgcacgtccg gcttgtcgat ccccatcccg
      601 aacgcgatcg tcgcgacgat gatctgaacg ttgtcgcgga cgaatgcctc ctggacgcgc
      661 tggcgttgct tgccgtccat cccaccgtgg taggcagcgg cgttgatgcc gtcgccgcgc
      721 agacggtcgg cgaggtcctc agtcgaggcg cgcgtctggc agtagacgat gccggacgca
      781 cccttgcgcg agtggacgta ttccagcaac tgcccgtatg ccgcttgctt cggccgcact
      841 tcgtagtaca ggttcggccg gttgaagctg ccctggaagc tgcgcgcccg ctccagcccg
      901 agctggtcgc ggatgtcctg ctggacacgc ggcgtcgctg tcgccgtgaa cgcggtgaag
      961 atcgcctccg gaaatcggtc gcggagctgg cgcagttcgc ggtactccgg ccggaagtcg
     1021 tgcccccact ccgagacgca atgggcctcg tcgatcgcga acatcgtgac gccacggtgc
     1081 tgtagcagat gcaggaatcc gggcaggacc agccgctccg gcgcgacgta caggagcttt
     1141 atgtcacccc gagcgacggc agcctgccgc tcccctgcct cgccggcgtc gagcgagctg
     1201 ttgataaacg tggcaggaac gccgagtgcc tgaagcgcat cgacctggtc cttcatcagc
     1261 gcgatcagcg gcgagacaac taccgtcagc ccatcgagca gcagcgccgg aagctggtag
     1321 cagagcgact tgccgccacc ggtcggcatc aggacgaaga catcctgccg gtcaaggatc
     1381 gcgttgacga tctcttcctg aagcgggcgg aacgtgcggt atccgaacgt cgattgcagc
     1441 gtagcggcga tctgttcggc ggttggatgt tcgccggctg gatcaccggc ccgtgccggc
     1501 tggcgtggtg tcgtctgcat gaagatatct ggctcctgct atggccgccg ccggcggccg
     1561 gggactggcg gtcctcagga ctatcaagga tacgccatct gccaattgtc cgaccgccga
     1621 tccggcatac aaattgcttc gctgcccggc aggaggaatc gatgccacga ggagacacgg
     1681 cactgaacca cctgcgcgca gaggccgcca gttgcacgcg ctgcccgttg cacgagatcg
     1741 gtacccaaac cgtcttcggc gccggaccgg ccagcgccag actgatgttc gtcggcgagg
     1801 cgcccggcgc gcaggaagac gcgaccgggc agccgttcgt cggtccggcc ggcaaagtct
     1861 tcgacgaagc gatcgccgct gctggtatcg accgggcgag cgtctacgtt accaacgcgg
     1921 tcaaacatcg tccgtggaag cgctcagaga gcggccggca gaagaaccgt gcccccaagc
     1981 agagcgagat caaagcctgc gccatctggc tggacggaga gatcgagatc atccagccgc
     2041 agttgatctg ctgcctggga gccgtcgccg ccaagcggct actgggaaaa gacttccggc
     2101 tgatggagca acgtgggcaa tggttctcgt cggacgatgg ccgggacgtt ctggcgaccg
     2161 tccacccctc gttcgtccag atccagcgac ctgaagcccg cgaacgctgg atgtcgacct
     2221 tcgtcgatga cctccgcgcg gtcaagcaac gccttgatga cgctcattag gacaggtcca
     2281 gtgacgcctg aagcggcgcg cacgcagacc aagcgttgcg tgccctcgtg ctagactgcc
     2341 cgtgttgaag tgcgggcgtg gattggcagg agtggcgacg atgctggcgt tgacggcggc
     2401 tgagattcgg gatctggttc cgatgcgaga cgcaatcgag ttgatgaaga gcgtcttcgc
     2461 ggccaactca cgtggcgaaa cgatcagccc gctccgaacg ccgatcaaca tgcccgatgg
     2521 tagcggtgtc gtcctgttca tgcccgccta cgttccgcca tcgtcaaccg cgccggccgc
     2581 cgcgggatcc aagatcgtct cggtcttcgc cgggaaccgt gatctgggac tcccaaccat
     2641 caacgcgatc gtcatcatgg tcgatccaac gaccggccag ccgcttgggc tgctcgaagg
     2701 agcgacgatc accgccctgc gcaccggcgc cgtctcaggg gccgccaccg acctgctggc
     2761 acggccagag agcacgaccc tggcgatcat cggagccggt gcccagggcg tgacgcaggc
     2821 cgccgccgtc tctgccgtgc gcgacattcg cgagatccgc gtgtcggacc tgagcgaggc
     2881 tgcccgcgcc aatttcgccg agcgacttgc cgtttgggcg ccagccatgg ccggcaaggt
     2941 ccgaccggcc gaaagcgccc aggaagccgt cgagggagtt gacatcatct gcacggcgac
     3001 aacggcgacg cgggctgtct tcgaggacgc ctggctgaag cctggcacac acatcaacgg
     3061 cattggcgca tacacgccgc agatgcagga gatcccggac gcgaccgtcg gccgcgcacg
     3121 catcgttgtc gacgccgtcg aggccgtcct ccacgaggcc ggtgatctga tccagccgat
     3181 cgagcgcggg gttctgaccg aagccgatat tcaggttgaa ctggggcacg tcgtcgatgg
     3241 cgtcgcagtc ggccgggaag acaacgagca gatcaccttc ttcaagtcag tcggcaatgc
     3301 gatccaggac atgatcgtcg gcgcggcggc cctcaaggct gccggagagc ggggtgtagg
     3361 gcagcagttg gcactcgaat aggacgcagt cgttcacgta actcgcgtag gggcgatggc
     3421 tgcggccatc gcccctacgc gagtagtccc aatcagacca ggatgttctg ttagccgtcg
     3481 gctgccaggg ccatatcgac gtgcgaatgc atcgggcagt tgccggtcgg gcagacaccg
     3541 ttgaggtgag cctcgaactc gtcccgatac gtcttcagca tgccgagcag cggcatcggg
     3601 gcgacctgac cgaggccgca gagtgatagt tcgatgacat gcttgctggc ctgctcgatc
     3661 agcatcaggt cggtcgggcg gccagtgccg aagcgcaggc ggtcgacgat ctcgaccaga
     3721 gccgggttgc cgagccggca gggcgtgcac ttgctgcatg actcgtagcg gttgaatgcc
     3781 tgcagataac gggcgaactc gaccgcgcag accgatttgt cgaagacgac gaagccaccg
     3841 gatccgagca tgccgccgag cggtgtcagc gagtcgaagt cgaccggcac gtcgagctgg
     3901 tcctcggtca gcgggccagc cgagacacca ccggtctgga cgcccttgaa ctcgtaaccg
     3961 tccttcatgc cgccatagac gttgaagatc aggtccttca ggttggcgcc catctcgact
     4021 tcgacgacgc cgagccgctt gagcggcccc tggaacgtga tcagtttggt gcctttgctc
     4081 ttctcagtgc ccatcgacgc gtaccagtcg ccaccgttct tgatgacgag cgcgacgttg
     4141 gcgatcgact cggtgttgt
//
LOCUS       DASUGJ010000022         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1113727, whole genome shotgun sequence.
ACCESSION   DASUGJ010000022 DASUGJ010000000
VERSION     DASUGJ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113727"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VFV93_00555"
     CDS             complement(<1..996)
                     /locus_tag="VFV93_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5163854.1"
                     /translation="MEHPATPGSQDLAAADGSRTLTLRGATVLGIGSMIGAGIFALLG
                     EAGAVAGAAVWISFLVGGVVALLLGYVCAKLGMRYPSSGGLITYLIKGFGLGRLVGVA
                     SWLGYIAAIVIVTAMVAVSFGGYATSLFVGQNASSGWNNLFITALLLVMLGVNLIGTK
                     VVAAAQSVLVVGVVAVFIIFICVTIPTIDTDLLAFDGYPSYSKIIASVALTFFAYLGF
                     NVITFTGGDLRNPRRDIPRATYLSLGITATIYILIAIGVFGTLTVTQVIDYGETAIAE
                     AARPTLGDAGFYIMAIVALLSTAGATNATLYASSNLTNMLASEGLFPEFFGSRSRF"
     gene            complement(1038..1403)
                     /locus_tag="VFV93_00560"
     CDS             complement(1038..1403)
                     /locus_tag="VFV93_00560"
                     /inference="COORDINATES: protein motif:HMM:NF025475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HEX5163855.1"
                     /translation="MANPSATLSRGAFDDDMQVETSGNFLGRLFDLSFTSFITPMLVR
                     ALFVLGLVVALIIAVGAVLVAFFESGIWAGIWALIWAPVWLLVVAVVLRIWLEICIVL
                     FRVAQASIEIARNTKPPRA"
     gene            complement(1631..2887)
                     /locus_tag="VFV93_00565"
     CDS             complement(1631..2887)
                     /locus_tag="VFV93_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5163856.1"
                     /translation="MATDPSIVSTSPDAFQLAQLKVPLDDADDAFAVRDDSGRIPIVI
                     VPTRPNRIRNDFVAYGLVLLLTTVIASLTLLPGWTSIPGTGLAILLIVLGVFRSFIVR
                     IPEGTNGLLARGGRYSRTVGSGSHIIPPWIAVSHVVTRREIPFDIPVVEATTKDTVRA
                     NVDSLLTFTIDDPYRFVFAISTDDFDHVLQASCQEAMRSLIRSITVSEVFNLTGRDTD
                     ELRATIGPDVAPYGIKIEKIKITYAQPPAEFMRSEESRQLAILQIAEQVERHALDERL
                     QRDADVLARQQQLARIQRHQDELESEREELDHRREIVEFEAQIEAIRLAKLDERLRSF
                     PEAVRYDVSRARLDVALALAGNSRAVLQIGSADDISEVLLVRDMWDETRATSNGQLPA
                     ESPADGARVEVPPILEPVVEAPEDGS"
     gene            complement(2893..>3882)
                     /locus_tag="VFV93_00570"
     CDS             complement(2893..>3882)
                     /locus_tag="VFV93_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5163857.1"
                     /translation="ILILRVLAIELYGTYQSLPTAPEIRERIQDLDQSMNAWPEPIRI
                     FVRTRALDTLLSIRERFVNYVSGVDGLAIDIVMGTVNAIGVALGLLVLPVWLLLALRD
                     QGSAREAINRHLPDWIEADFWAVARILDRTLGTYIRGLGLIALSIGIGTFAGLWTLDA
                     AGAEGIRYPVALGLLVGMLELIPTIGPMISTLAVFLIMLRESPESAIAAVLVVLAVRL
                     LVRRFVTSRIERRVIDAHPAILIIVLVALSQFGIVWAIFAAPLVAIARDLFRYTYGRL
                     SDPPSPTGVLPGEPAPAMIHAVPARRHRPLVYQRASVARAPAPLDPVEQAVGE"
BASE COUNT          769 a         1153 c         1309 g          651 t
ORIGIN      
        1 aaagcgtgac ctggagccga agaactcggg gaagagtccc tcgctcgcga gcatgttggt
       61 caggttcgac gacgcgtaga gggtcgcgtt ggtcgcaccc gcggtcgaga gcagcgcgac
      121 gatcgccatg atgtagaagc cggcgtcccc gagcgtcggc cgcgccgcct cggcgatcgc
      181 cgtctcaccg tagtcgatca cctgggtaac cgtcagcgtc ccgaacacgc caatcgcgat
      241 caggatatag attgtcgcgg tgattccgag cgagagatag gtcgctctgg gaatgtcgcg
      301 gcgcggatta cgcagatccc caccagtgaa ggtgatcacg ttgaagccga ggtaggcgaa
      361 gaacgtgagt gccacgctgg cgatgatctt cgagtacgag gggtagccat caaacgccag
      421 caggtccgtg tcgatggtgg ggatcgtcac gcagatgaag atgatgaaga ctgcgacgac
      481 gccgacgacg aggacggact gcgcggcggc gacgactttg gtgccgatca ggttcacacc
      541 gagcatgacg agcagcaagg ccgtgatgaa gaggttgttc cagccgctgc tcgcattctg
      601 accgacgaag agcgaggtcg cgtagccccc gaacgagacg gcgaccatcg ccgtgacgat
      661 cacgatcgcg gcgatatagc cgagccacga cgcgacaccg accagccgcc ccagcccgaa
      721 gcctttgatc aggtaggtga tcagcccgcc ggaggagggg tagcgcatac ccagcttcgc
      781 gcagacgtag ccgagcagga gcgcgacgac accgccgacc aggaaggaga tccagacggc
      841 agctcccgcg acggctccgg cttctccgag gagagcgaag atccccgcgc cgatcatcga
      901 gccgatcccc aggacggtcg caccgcgtag tgtcagcgtc cgggaaccgt cggcagcagc
      961 gagatcttgc gagccaggcg tcgcgggatg ctccatcagg cacactcctc atgcggcgag
     1021 tgtcgtctgg attgctgtca ggcccgcggg ggcttggtgt tgcgggcgat ctcgatcgag
     1081 gcctgtgcga cgcggaacag gacgatgcag atctcgagcc agattcgcag cacgacagca
     1141 acgaccagca gccataccgg agcccagatc agtgcccaga tgccggccca gatgccactc
     1201 tcgaagaacg cgacgaggac ggcgcctacc gcgatgatca gggcaaccac gaggcccagg
     1261 acgaagagcg cccggacgag cattggcgtg atgaacgacg tgaatgacag gtcgaacagc
     1321 cggcccagga agtttcctga cgtctcgacc tgcatgtcgt cgtcaaaggc tccgcgcgac
     1381 aacgtagcgg atggattggc catctgtaga tccctttcca gcaaacgaaa tcgacgctcg
     1441 agccggtcat gtcactgcgg gaggagcgcg tcaaccgggc gtctactgaa cattgccgcc
     1501 gcaactgatt cgccatccct ggacatgatg tggcgtcatc cgatggtgct agcgtagtct
     1561 ggctgtgcct gtcgtgaatc gtccggtagt ggtaatccag ggtcgtagtc agggagcgct
     1621 tctggcgtga ttacgagccg tcttctggcg cttcaacgac cggttcaagg atgggtggaa
     1681 cctcgacgcg tgctccatcg gcaggcgact cggccggcaa ttgtccattg gaggtcgcac
     1741 gcgtctcgtc ccacatgtcg cgcaccagca ggacctcgct gatgtcgtcg gcactaccga
     1801 tctggaggac tgcacgggag ttgccggcca gcgcgagggc gacatcgagg cgggctctgc
     1861 tgacgtcata ccggacggcc tcggggaacg aacgtagccg ctcgtcgagc ttcgccagcc
     1921 gaatcgcttc gatctgcgct tcgaactcga cgatctcccg gcgatggtcc agttcctccc
     1981 gttcactctc gagctcgtcc tggtggcgct ggatgcgcgc gagttgctgc tggcgggcga
     2041 ggacgtcggc gtcacgttgc agacgctcgt cgagcgcgtg tcgttcgacc tgttcggcga
     2101 tctggaggat cgcgagctgc cggctctctt cggagcgcat gaactcggcc ggaggctggg
     2161 cgtaggtgat cttgatcttc tcgatcttga ttccatacgg agcgacatcc ggaccgatcg
     2221 tggcgcgtag ctcatcagtg tccctgcccg tcaggttgaa tacctcggaa accgtgatgc
     2281 tgcggatgag cgagcgcata gcttcctggc acgaggcctg gagcacgtga tcgaagtcgt
     2341 cggtcgagat ggcgaacacg aatcgatacg ggtcgtcgat cgtgaaggtc agcagcgagt
     2401 cgacgttggc gcggacggtg tctttagttg tcgcctcgac caccggaatg tcgaacggga
     2461 tctctcgtcg ggtcacgacg tgggacacgg cgatccacgg cgggatgatg tgactcccac
     2521 tgccaaccgt ccggctgtag cgtcctccac gtgcgaggag tccgttggtc ccttctggaa
     2581 tgcgaacgat gaacgagcga aagacgccca gtacgatcag gaggatcgcg aggccggtgc
     2641 cggggatcga tgtccagccg gggagcaatg tcaggctggc gatgaccgtg gtgagcagca
     2701 gcaccagtcc gtaggcgacg aagtcgttgc ggatccggtt cgggcgggtc ggcacgatca
     2761 cgattgggat ccggccgctg tcgtcacgga ccgcgaaggc gtcgtcggcg tcgtccaacg
     2821 gcaccttgag ctgtgccagc tgaaacgcgt ccggtgatgt ggaaacgatg ctcggatcgg
     2881 tagccatgtc catcactctc cgacggcttg ctcaaccgga tcgagtggcg caggggctcg
     2941 tgccacgctg gcgcgctggt agacaagtgg gcgatgccgt ctggctggta ccgcgtgtat
     3001 catcgctggt gccggctcgc ctggcaggac gccggtcggg cttggcggat cgctgaggcg
     3061 gccgtatgtg tagcggaaca ggtcgcgcgc gatggcgacc agcggcgccg cgaagatcgc
     3121 ccagacgatg ccgaactgac tcagggcgac gagcacgatg atgagaatcg ccggatgggc
     3181 gtcgatcacg cgacgttcaa tgcgcgacgt aacgaacctg cgcacaagca gccgaacggc
     3241 gagcacgacg agcacggcag caatagcgga ttcgggtgat tctctcagca tgatcaggaa
     3301 gacggccagg gtgctgatca ttggaccgat cgtcggaatc agctccagca tgccaaccag
     3361 gaggcccaat gcgaccgggt agcggatacc ctccgctccg gcagcgtcga gcgtccagag
     3421 gccggcgaat gtgccgatgc cgatcgagag agcgatcagc ccgaggccgc gaatgtaggt
     3481 tccgagcgtc cggtcgagta tgcgggcgac agcccagaag tcagcctcaa tccagtcggg
     3541 cagatgccga ttgatcgcct cgcgagcact gccctggtct cgcagcgcca gcagcagcca
     3601 gaccggcaga acgagcaggc cgagggcaac gccgatcgcg ttgacggttc ccatcacgat
     3661 gtcgatcgcc aggccatcaa caccggagac gtagttgaca aagcgttcgc gaatcgacag
     3721 caacgtgtcc agcgcacgtg tgcgaacaaa gatccggatt ggttccggcc aggcattcat
     3781 cgactggtca aggtcctgga tgcgctcacg gatctccggg gcggtcggca gcgactggta
     3841 ggtgccgtag agctcgatcg ccaggacgcg caggatgaga at
//
LOCUS       DASUGJ010000023         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1669541, whole genome shotgun sequence.
ACCESSION   DASUGJ010000023 DASUGJ010000000
VERSION     DASUGJ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669541"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VFV93_00575"
     CDS             <1..1240
                     /locus_tag="VFV93_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEX5163858.1"
                     /translation="RGIHRHVVRFVNYDGTLYALKELPDRIAEREYRLLRRLADEEIP
                     VVTVIGIVSGRSTGDGVPGTAPRASDEELSGVLITRHLEFSLPYRTLFTGHSIPRLRD
                     RLLDALAELLVRLHLANVFWGDCSLSNTLFRRDAGELEAYLVDAETGEIHPTLSDQMR
                     RYDIDIAEENVAGELMDVQAGYGLPDEIDPVETAGSLRRRYESLWTELTHEETFGPDE
                     RYKIDARLRRLNDLGFDVDEMELITTPEGYRLRMHTQVVEPGHHRRRLMILTGLDVQE
                     NQARRLLYDIDSFRQAIERKEGRPLPESVVAYRWLGEVFEPTIAAIPDEMRGKLQPAE
                     IYHQVLEHRWFMSESEDKDVGLRRAVRSYINDVLRHVPDEAHVLTDEDDDAPDEPNDL
                     TEPAESPPGVLNTQEPADGR"
     gene            1230..1799
                     /locus_tag="VFV93_00580"
     CDS             1230..1799
                     /locus_tag="VFV93_00580"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5163859.1"
                     /translation="MDDDTTTELQCFFAALSDEERLRVAGVLAGRDATVQELAATLKL
                     RPPQIARHLAALTEAGLVRSTPDGALTRWSLDVAALREQRKRLLTRERAPSPADEPGT
                     PEWERSVLASFFDGERLKEIPANLRKRQVILAWLAGRFDPDARYPEREVNEIIKRHHP
                     DASALRRELVDHRYMQRENGVYWRLPSAS"
     gene            complement(1821..>3381)
                     /locus_tag="VFV93_00585"
     CDS             complement(1821..>3381)
                     /locus_tag="VFV93_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5163860.1"
                     /translation="VMLAIQTGKVTLHVDIASEAVARAGLTDARVEVIAALGFRSVIV
                     VPLEARGRRIGALSLMYADSGRRYSHTDLSVALELAHRAATAVDNALLYHEAQQAAAR
                     LRALAEASHAFAEASLDLPAVLDAVARSAGELIGDQCILRLLSDESDLLPAVAVYHPD
                     GERRAQLAEFLAREAQHANEGLVGSVVATGRPVFIPATDLDALRTTITAGHVEDIERV
                     PLHSIIIVLVRVHERPIGTLGLGRERPGEPYTEEDLLLLQELADRAGLAIENARLFRE
                     AQDAIRSREQFLSIASHELKTPLTSVKASAQLLGRRMQRDPIDRERLMPLVAQVQSEI
                     GRLENLVHDLLDATRIQQGRLELRPEPVDLVDLARQALGRFESAAQQAPVHTLRLDAP
                     EPIIVEIDPDRIDQVITNLISNALKYSPDGGDVGVAVRRIDSVAEIAVSDAGIGISQA
                     EQATLFQPFARGDVARQSIGGTGLGLFISAEIVERHGGTIEVESTPGHGSTFTVRLPL
                     DAHDPNSRATT"
BASE COUNT          581 a         1157 c         1124 g          519 t
ORIGIN      
        1 acgggggatt caccgccacg tcgtccgctt cgtcaactat gacgggacgc tctacgcact
       61 caaggagttg cccgaccgga tcgcggagcg cgaataccgg ctgctacggc gcctggccga
      121 cgaggagatc ccggtcgtga cggtgatcgg catcgtctcg ggccgctcga ccggcgacgg
      181 ggtgccgggc acagcgcccc gggccagcga cgaagagctc tccggcgtcc tgatcacccg
      241 ccatctggag ttctcgctgc cctaccggac gctcttcacc ggccacagca tcccgcgcct
      301 gcgtgaccgg ttgctggatg cgctggcgga gctgctggtc cgactccacc tcgccaacgt
      361 cttctggggc gactgctcgc tctccaacac gctctttcgc cgtgatgccg gcgagctgga
      421 ggcgtatctg gtcgacgcgg agaccggcga aatccacccg accctctctg accagatgcg
      481 ccgctacgac atcgacatcg ccgaggagaa cgtggccggg gagttgatgg acgtccaggc
      541 cggctacggt ctgcccgacg agatcgaccc ggtcgagacg gccggcagcc tgcgccgccg
      601 ctacgagagc ctctggacgg aactgaccca cgaagagacc ttcggccccg acgagcgtta
      661 caaaatcgac gcccggttgc ggcggctcaa cgacctcggc ttcgacgtcg atgagatgga
      721 gctgatcacg acgccggagg gctatcggct gcggatgcac acccaggtcg tcgagccggg
      781 ccatcaccgc cggcgattga tgatcttgac tggtcttgat gtgcaggaga accaggcccg
      841 gcggctactc tacgacatcg acagtttccg tcaggcgatc gagcgtaagg aggggcgccc
      901 gctgccagag tcggtcgtcg cctatcgctg gctgggcgag gtcttcgagc cgacaattgc
      961 cgccatcccc gatgaaatgc gcggcaagct ccagccggcc gagatctatc accaggttct
     1021 cgaacaccgc tggttcatgt cagaatccga agacaaggac gttggtctgc ggcgggccgt
     1081 ccgctcgtat atcaacgacg tcctgcgcca cgtcccggac gaggcacacg tcctgaccga
     1141 tgaagacgac gatgcgccag acgagccgaa cgatctgacc gaaccggccg agtcgccgcc
     1201 gggcgtgctg aacacacagg agccggccga tggacgatga cacgacaacc gagctgcaat
     1261 gcttcttcgc cgccctgtcg gacgaggagc gcctgcgcgt ggcgggcgtc ctggccggcc
     1321 gcgacgcaac cgttcaggaa ctggcagcca ccctcaagct gcgcccgccg cagatcgccc
     1381 gccacctggc ggcgctgacc gaagcgggac tcgtccgctc gacaccggac ggcgcgctga
     1441 cgcgctggtc gctggatgtc gccgccctgc gtgagcagcg taagcgactg ctgacacgcg
     1501 aacgggcgcc ttcgccggcc gacgagcccg gtacgccgga gtgggaacga tcggtgctgg
     1561 ccagcttctt cgacggtgag cggctgaagg agattcccgc caacctcagg aagcgtcagg
     1621 tcatcctggc ctggctggcc gggcgattcg accccgacgc gcgctacccc gaacgggagg
     1681 tcaacgagat catcaagcgg caccaccctg acgcgtccgc cctccgccgc gaactcgtcg
     1741 accaccgcta catgcaacgc gaaaacggcg tctactggcg cctgccatcg gcgtcgtaga
     1801 gacaggtctg agacctggcc ctacgtggtg gcgcgcgagt tcggatcatg cgcatcgagc
     1861 ggcagccgga cggtgaaggt gctgccgtgg ccgggggtgc tttcgacctc gatcgtgccg
     1921 ccgtggcgtt ctacgatctc ggccgagatg aacagcccca ggcctgtgcc gccgatgctc
     1981 tggcgggcga cgtcgccgcg ggcgaacggc tgaaagaggg tcgcctgttc ggcctggctg
     2041 atcccgatgc cggcatcgct gaccgcaatc tcggccacgc tgtcgatccg gcggaccgca
     2101 acaccgacat caccgccgtc cggcgaatac ttcaacgcgt tcgagatgag gttggtgatg
     2161 acctgatcga tccgatcggg gtcaatctcg acgatgatcg gttccggagc atccagcctg
     2221 agcgtgtgga ccggggcttg ctgggcagcg gattcgaagc gccccagcgc ctggcgggcg
     2281 aggtcgacca gatcaactgg ttccggccgc aactccagac gcccctgctg aatgcgggtg
     2341 gcgtccagca gatcgtggac gaggttctcc aaccggccga tctccgactg aacctgcgcc
     2401 accagcggca tgagccgctc gcggtcgatg ggatcgcgct gcattcgccg gccgagcaac
     2461 tgggcggacg ccttgaccga ggtcagcggc gtcttgagct cgtgcgaggc gatcgagagg
     2521 aactgctcgc gcgatcggat ggcgtcctgc gcctcacgga agaggcgggc gttctcgatc
     2581 gccagaccgg cccggtcggc cagttcctgt agcaacagga ggtcttcctc ggtgtacggc
     2641 tcgccgggcc gctcgcgacc gagcccgagc gttccgatcg gccgctcgtg gacccggacc
     2701 aggacgatga tgatgctgtg caacggtacc cgctcgatgt cctcgacgtg accggcggtg
     2761 atcgttgtgc gcaacgcatc cagatctgtc gccgggatga agaccggccg gccggtcgca
     2821 acgaccgacc caaccagacc ctcgttggcg tgctgcgcct cgcgggccag gaactcggcc
     2881 agctgcgcgc ggcgttcccc gtccggatga tagacggcca ccgccggcag caggtcgctc
     2941 tcgtccgaca gcaagcgcag gatacattga tcgccgatca gctccccggc tgatcgggca
     3001 acggcgtcaa ggacggccgg cagatccagg ctcgcctcgg cgaaggcatg cgaggcctcg
     3061 gccagcgccc gcagccgggc tgccgcctgc tgggcctcgt gatagagcag cgcgttgtcc
     3121 accgccgttg ccgcgcgatg agcgagttcc agcgcgaccg acagatcggt atgactgtag
     3181 cgccggccgg agtccgcgta catcagcgag agcgcgccga tccgccgtcc acgcgcctcg
     3241 agcgggacga cgatcaccga gcggaacccc agcgcggcga tgacctcgac ccgcgcatcg
     3301 gtcaacccgg cgcgagcgac cgcttcactg gcgatatcca catggagcgt caccttgccg
     3361 gtctggatcg cgagcatgac c
//
LOCUS       DASUGJ010000024         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_836417, whole genome shotgun sequence.
ACCESSION   DASUGJ010000024 DASUGJ010000000
VERSION     DASUGJ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836417"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VFV93_00590"
     CDS             complement(<1..1216)
                     /locus_tag="VFV93_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5163861.1"
                     /translation="MVATSRTQPRPATASLNPFLREPQNIDETGLDLSFISDLIIKVI
                     YFNSMTTAQAIADTLCLPFFNVVDRALTLLKREEMIEVVGSSGFGELAYQYLVTPKGN
                     ARAHEVISRSAYVGPAPVTLEAYKEVVKSQAIGNVKVTPQDVRRATADLIVPDEIIDV
                     LGQAVTTGRSLFLYGEPGNGKTVLAERVTELLGGSILIPHAVTVDGQVIKVLDLHNHI
                     PVGLPGGKTDIDRRWVICKRPYIVVGGELMLQSLDLVWDNANKFYEAPFQMKANGGMF
                     MIDDFGRQQVRPSELLNRWIVPLEKRIDFLTLHTGKKIEIPFDEIIVFSTNLAPKDLV
                     DEAFLRRIQCKVKIDNPTLDNYREIFRRQCDALSVPFDQNGLVYLLREYYVKPKRELR
                     ACHPRDILRTLTG"
     gene            complement(1393..>2285)
                     /locus_tag="VFV93_00595"
     CDS             complement(1393..>2285)
                     /locus_tag="VFV93_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5163862.1"
                     /translation="AVAERGSLDVTIQTVGTLQATGATTVRSIQPGIVDTLGVRAGDI
                     VEQGDIIALLDMEPFDRNVQQAQRELDQSEFALQAAEQQAQANADDPGLRLDVLNAAD
                     RVERATAALDQANEARRNAVIVSPSAGTVVELTVRSGDGVGANQSIARVATRDDLTIL
                     ADVDELDLPNVALGAEARFRLDAFPSTEITGRVVNTAPQARQQGGATVFQTEIEFDPP
                     EDLDLRPGMNADVTIVTASRDNVLLIPEAALRTVGDRSFVLVVTDRDTEEREVELGYR
                     GNGQVEVVSGLTDGEQVVLR"
BASE COUNT          394 a          692 c          746 g          453 t
ORIGIN      
        1 tgccggtcaa cgtccgcagg atgtcgcggg gatgacaggc gcgcagctcc cgctttggtt
       61 tgacgtaata ctcacgaagc agatagacga ggccgttctg gtcgaacggc acgctgagcg
      121 cgtcgcactg ccgccggaag atctcacgat agttgtcgag cgtcgggttg tcaatcttga
      181 ccttgcattg aatgcggcgc aggaatgcct cgtcaacgag gtctttcggc gccaggttgg
      241 tcgagaagac gatgatctcg tcgaagggga tctcgatctt cttaccggta tggagcgtca
      301 ggaagtcgat ccgcttctcc agcgggacga tccagcggtt gagcagctcg ctcggccgca
      361 cctgctgccg gccgaagtcg tcgatcatga acatgccgcc gttggccttc atctggaacg
      421 gcgcctcgta gaacttgttg gcgttgtccc agaccaggtc gaggctctgc agcatcagct
      481 caccgccgac gacgatgtag gggcgcttgc agatgaccca gcgacgatcg atgtcggtct
      541 tgccgcccgg caggccgacc gggatgtggt tgtgcaggtc gagaaccttg atgacctggc
      601 catcgacggt gactgcgtgc gggatcagga ttgagccgcc gagcagctcg gtcacacgct
      661 cggcgagaac cgtcttgccg ttgcccggct cgccatacag aaacagcgac cgtccggtgg
      721 taacggcctg cccaaggacg tcgatgatct cgtcggggac gatcaggtcg gccgtcgccc
      781 ggcgcacgtc ctgcggcgtc actttgacgt tgccgatcgc ctgtgacttg acgacttcct
      841 tgtaggcttc gagcgtgacc ggagcagggc cgacgtaggc gctgcggctg atcacttcgt
      901 gcgcgcgggc gttgcctttc ggcgtcacca ggtactggta cgccagttcg ccgaagccgc
      961 tcgaaccgac gacctcaatc atctcctcac gcttcagcag cgtcagtgcc cggtcaacga
     1021 cattgaaaaa tggtaggcag agtgtgtcgg cgatcgcctg ggcagttgtc atcgaattga
     1081 aatagatgac tttgataatg aggtcggaaa tgaaactgag atcgaggccg gtctcgtcga
     1141 tgttctgcgg ctcccgcaga aatggattga gagaggcggt agctggcctc ggctgagttc
     1201 tggaggttgc gaccatctcc aaccttccca tttccgaact agttgctaat gacacccgtc
     1261 ggcaccgttt tgcgcgacaa cacaagcgta ggcacccacg cgatgaggtg cgatagcaat
     1321 gcagtcctga ttgtcacgaa cggttatcgg gggcccgagt acctggatac tatgttcggt
     1381 tgacgcgaat attcaccgca gcacgacctg ctcgccgtcg gtcagccctg agacgacttc
     1441 gacctgcccg ttgccccgat agccgagttc gacttcgcgc tcctcggtgt cgcggtcggt
     1501 cacgacgagg acaaacgagc gatcgccaac ggtccgcaag gcggcttcgg ggatcagcag
     1561 cacgttgtcg cgcgaggccg tcacgatcgt cacgtccgca ttcatgccgg gacgcaggtc
     1621 gagatcttcc ggtgggtcga actctatctc ggtctggaag acggtcgcgc cgccctgctg
     1681 gcgcgcctgt ggagcggtgt tgacgacacg gccggtgatc tcggtagatg ggaaggcatc
     1741 gagccggaag cgcgcctcgg cgcccagcgc gacgttgggg agatccagct cgtcgacatc
     1801 ggccaggatc gtcaaatcgt cccgcgtcgc tacccgggcg atggactggt tcgcgccgac
     1861 gccgtcgccg ctgcggacgg tcagttcgac gactgttccg gccgacgggc tgacgatcac
     1921 cgcattgcgg cgcgcctcgt tggcctgatc gagcgccgcc gttgcccgct cgacccggtc
     1981 ggccgcgttg aggacatcca gccggagtcc ggggtcatcc gcgtttgcct gtgcctgctg
     2041 ttcggctgcc tggagcgcga attcggactg atctagttcg cgttgggctt gctggacgtt
     2101 gcggtcgaac ggttccatgt cgagcagcgc gatgatgtct ccctgctcga cgatgtcgcc
     2161 ggcccggaca ccgagcgtgt cgacgatgcc tggctggatc gagcgaacgg tcgtcgcgcc
     2221 ggtcgcctgg agcgtgccga ccgtctggat cgtcacatcg agcgacccgc gttcggcaac
     2281 ggccg
//
LOCUS       DASUGJ010000025         3517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1253342, whole genome shotgun sequence.
ACCESSION   DASUGJ010000025 DASUGJ010000000
VERSION     DASUGJ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253342"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFV93_00600"
     CDS             <1..288
                     /locus_tag="VFV93_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163863.1"
                     /translation="VTGIRLRGAMLLLWLLVPLFALAACICVAGPGRLFNKRTHEGRT
                     VVLLTPNDAITTLDLIGLGLAALGILLALALIGWRLHASSLLRSRRFVLRH"
     gene            421..1104
                     /locus_tag="VFV93_00605"
     CDS             421..1104
                     /locus_tag="VFV93_00605"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5163864.1"
                     /translation="MQQVAQVEIPSVELGYSGWYKASKRLFDLVVGSLLILCLLPVFI
                     VCAFLVWRSSPGPIIFRQPRVGVRGREFTFLKFRSMRVDADPAIHREYVAKFINGAAE
                     QQPAGNGSMFKLVEDPRVTRVGRVLRRTSLDELPQLFNVIRGNMSLVGPRPPIPYELE
                     HYRPEHYHRLAVKPGMTGLWQVSGRSHTTFDEMIALDIEYIHRASFLTDLRLLFRTIP
                     VVLGQHGAQ"
     gene            1147..2229
                     /locus_tag="VFV93_00610"
     CDS             1147..2229
                     /locus_tag="VFV93_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5163865.1"
                     /translation="MDRVLPNGPLTIGVVGYGYWGPKLVRNFYEIPDVTLGWVVDKDP
                     GRLSKVSANYPGVRTTDSFDDLLGSDADAVVIATPIRTHYPLAKAALQAGKHVLVEKP
                     LTASSFEADELARLAEDRGLTLMVGHTFEYNAAIRKLREIVASGELGDIYYVDSSRLN
                     LGLFQPDINVLWDLAPHDLSILLYVLGRDPLEVSARGSANVRQGIHDVAYIEVRFPDN
                     ILAHLHLSWLHPCKVRSVTIVGSRKMVVCDDLSDSEKIRIYDKGVDCQYETDQFNDFH
                     LQYRYGSIHIPYVPFEEPLRVQCEHFLGCIQTGTRPQSDGRVGAKVVRILEQADKSLL
                     NGGGRESLGLDFNAALAQGLLQAVAD"
     gene            2247..3422
                     /locus_tag="VFV93_00615"
     CDS             2247..3422
                     /locus_tag="VFV93_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5163866.1"
                     /translation="MATEVILPKLGMNTETATILSWQKQEGETVAVGDVLAEVETDKA
                     VIELEAEAAGVLRRWLAAEGDQVAVNAAIALIGSASEDLTAIEQKLSRATPATVTHVE
                     HIYQTWKAPDQAIAPPTNGHHSGNGSVASADRKPTPEPRLDPNAIRARLAKRGILGDG
                     PATEVSRTRLVIYGSGLGAKQLLEVTRLLEDVEVVGLIDDNPDLLGVTLGGARVLGGF
                     SALAELAARHEIDGVALSFHSEVRKKVHERIRAELDIRILPLVDPRAVVGMDVTIADG
                     ALIEAGAVIGPGTVVGEGTIVDVGVTVAHDCLLGPFSHLSPGCALSGVVCLKGNVLVG
                     VGAALNSTITVGRGVIITPGSAVMNDVPDNVVVSGVPARNIGASRRDRAGQGEPDGA"
BASE COUNT          600 a         1179 c         1127 g          611 t
ORIGIN      
        1 gtcaccggca tccgcctgcg cggagcgatg ttgctgctct ggctgctggt gccgctgttt
       61 gccctcgcgg cctgcatctg cgtggccggt ccagggcggt tgttcaacaa gcgcacccac
      121 gagggacgta cagtcgtcct gctgacaccc aacgacgcca tcacgacgct cgatctgatc
      181 ggcctcggcc tggctgcact cggcatcctg ctcgctctcg cgctgattgg ctggcggctg
      241 cacgcatcca gcctgcttcg atcgcgccgt tttgtcctgc gccactgacc ctccgccggc
      301 tcaaccgcat cttgacagcc agcacaccgg cgcttcacgg gcgctaccta tcgtcagaat
      361 cacggattcc ggtcaccgtc agccggtctg acagcagcgc gtcgctgctg cggagggcgt
      421 atgcagcagg tagcgcaagt tgagattccg tcagtcgagc tgggatacag cggctggtac
      481 aaggcatcca agcggctctt cgacctcgtt gtcgggtcgc tgctgatcct ctgcctgctg
      541 ccggtcttca tcgtctgcgc gttcctcgtt tggcgcagtt cgccggggcc gattatcttt
      601 cgccagccac gcgtcggcgt tcggggacgc gagttcacct ttctgaagtt ccgctcgatg
      661 cgcgtcgacg ccgacccggc catccatcgc gaatacgtcg cgaagttcat caacggcgcc
      721 gccgagcagc agccggccgg caacggctcg atgttcaagc tggtcgagga tccgcgcgtg
      781 acgcgggttg ggcgtgtcct gcggcgcacc agcctcgatg agctgccgca actgttcaac
      841 gtcattcgcg gcaacatgag tctggtcggg ccacggccgc ccattcccta cgagctggag
      901 cattatcgcc ccgagcacta tcaccggctg gccgtcaagc cgggcatgac cgggctctgg
      961 caggtcagcg gccgcagcca taccaccttc gacgagatga tcgcgctgga tatcgagtac
     1021 atccaccgcg cctcattcct gaccgacttg cgcctgctgt tcaggacgat tccggtcgtc
     1081 ctcggccagc acggcgcgca atagcccagg cgagacgctt gcgtccgcta cggagggagt
     1141 ctgtccgtgg atcgtgtcct cccgaatggt ccgttgacga tcggcgtcgt cggctatggg
     1201 tactgggggc caaagcttgt ccgtaatttc tatgagatcc ccgatgttac gctcggctgg
     1261 gtcgttgata aagatccggg ccgcctcagc aaggtcagcg cgaactaccc cggcgtcagg
     1321 acgaccgaca gtttcgacga tcttcttgga tccgacgccg atgccgtcgt gatcgcgacg
     1381 ccaatccgca cccactaccc attggcgaaa gccgccctcc aggccggcaa gcacgtactg
     1441 gttgagaaac cgttgacggc cagtagcttc gaggccgatg agctggcccg gctggccgag
     1501 gatcgcgggc tgacgctgat ggtcggccat acgttcgagt acaacgccgc catccgcaag
     1561 ctgcgcgaga tcgttgccag cggcgagttg ggcgacatct attatgtcga ttcatcccgg
     1621 ctcaacctgg gcctcttcca gccggacatc aacgtcctct gggatctggc gccacacgac
     1681 ctgtcgatcc tgctctacgt cctcggccgc gacccactgg aagtcagcgc gcggggcagc
     1741 gccaacgtcc gccagggaat tcatgacgtc gcctacatcg aggtgcgctt ccccgacaac
     1801 atcctcgccc acctgcacct gagctggctg cacccctgca aggtccgcag cgtcacgatc
     1861 gtcggcagcc gcaagatggt cgtctgcgac gacctctccg acagcgagaa gatccgcatc
     1921 tacgacaagg gcgtcgactg ccagtacgag acggaccagt tcaacgactt ccacctgcaa
     1981 taccgctacg gcagcatcca cattccctac gtccccttcg aggagccgct gcgggtccag
     2041 tgcgagcact tcctgggctg catccagacc gggacgcgtc cgcagagcga cggccgggtg
     2101 ggagccaagg tcgtccggat tctcgaacag gccgacaagt cgctgctgaa cggcggcggg
     2161 cgcgaatcac tcggtctcga cttcaacgcg gcgctggcgc aggggctgct ccaggccgtt
     2221 gcggactgac aggttggata gatagcatgg ctactgaagt catcctgccg aagctgggga
     2281 tgaacaccga aacggcgacg atcctcagtt ggcagaagca ggaaggcgag accgtcgctg
     2341 tcggcgatgt gctggccgag gtcgagaccg acaaggccgt catcgagctc gaggctgaag
     2401 cggccggcgt cctccgtcgc tggctggcgg ccgagggcga ccaggtagcc gtcaacgctg
     2461 ccatcgccct gatcggcagc gccagcgagg acctgaccgc gatcgagcag aagctctccc
     2521 gcgcgacgcc ggcaacggtc acccacgtcg agcacatcta ccagacgtgg aaagcgcctg
     2581 accaggctat tgcgcccccg accaacggcc atcactcagg aaacggaagc gtagcgtcgg
     2641 ccgatcgcaa accaaccccc gaaccccgcc tcgaccccaa cgcgatccgc gcccggctgg
     2701 ccaagcgggg catcctcggc gatggcccgg caaccgaggt cagtcggacc aggctggtca
     2761 tctacggcag tggcctcggc gcgaagcagc tgctggaagt cacgcggctg ctggaggatg
     2821 tcgaggtcgt cgggctgatc gacgacaacc ccgacctgct cggcgtgacg ctgggcggcg
     2881 cgcgggtgct gggcggtttc agcgcgctgg cagagcttgc cgcgcggcat gagatcgacg
     2941 gcgtcgcgtt gtcgttccac tcggaggtcc gcaagaaggt ccacgagcgg atcagggccg
     3001 agctggatat ccgaatcctg ccgctggtcg accctcgggc cgtggtcggc atggacgtga
     3061 cgatcgcgga cggcgcgctg atcgaggccg gggccgtcat cggaccgggg acggtcgttg
     3121 gcgaaggcac gatcgtcgat gtcggcgtca ccgtcgccca cgactgcctg ctgggaccgt
     3181 tttcgcacct gtcgcccggc tgcgcgctct ccggcgtcgt ctgcctgaag ggcaacgtcc
     3241 tcgtcggcgt cggcgcggct ctcaactcaa ccatcacggt cgggcgcggg gtcatcatca
     3301 ctccggggtc ggccgtcatg aacgacgtgc cggacaacgt cgtcgtcagc ggcgttccgg
     3361 cgcggaacat cggggccagc cggcgggatc gcgcagggca aggagaaccc gatggagcat
     3421 gagacccaga cgtatatcga ctactaccgg ctgatgctgc gcatccggcg ctgcgaggaa
     3481 cggatcggct ggctctttgc gcgcggcctg acgatgg
//
LOCUS       DASUGJ010000026         6242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1531409, whole genome shotgun sequence.
ACCESSION   DASUGJ010000026 DASUGJ010000000
VERSION     DASUGJ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531409"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFV93_00620"
     CDS             <1..376
                     /locus_tag="VFV93_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5163867.1"
                     /translation="VIAVQSEGMPVVYRSFHERQLLSLEGGSTWAEGLATRVAFELPY
                     AMMQELVDDVQTVSEEEMRQAMLVLLEQAHIVAEGAGAASLALATRLAPELRGQRVGL
                     IVSGGNVTRETLRRAMSDDGSW"
     gene            457..2547
                     /locus_tag="VFV93_00625"
     CDS             457..2547
                     /locus_tag="VFV93_00625"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5163868.1"
                     /translation="MLEQFSRWLRRYQGTEANPIYRLAVDVATGRMSFERALDAAESH
                     SVNGRLADGDLVDLDQQVESEARVNLQFALILARLNAATARAKGFEKVLVDLYLRIAE
                     LLEDERDEAEREYNYREALHVAKRISYVAGQRRALNRLARMAFERGDTEDARGLLLQQ
                     LEAGREESDTGDEIETAILLADISLADGDVSTAHDLYHRAARSARRIGFHTGAVDALL
                     RQVGILRERGDLHASLQLLRQASESADRTVDTALQAEIAFRTGALMRELNMPEEATER
                     LTIALQRARTLGDMAMEARSLLMLAQLEQRMGRDEQALQHYEAAVGLERGLGNRGEVA
                     RALQAMGEIHLAQDRPSAAISVLTEAREMVLYSDDTELLMQVHGMLGKALADQGRERE
                     ALDALQTALASSRALDDPTEEARWIITVAEVMLRTGETDNAGELAQRAEQLARGSSNN
                     VLRAAVYSLNGQVALVDRRLQDAEDAFASATAASRAAGQSGASLRYLPLLSRLAAELG
                     DMDGAIRYLDEAVAEAQAVGEDAQACMFHGQAARLLGSAGRIRDAEERYQRALALGRS
                     AGDPRLSGRALQGLAAIYDTTGQLELAIEHYYDALDAANRAGDARTIASTHFNLGALL
                     VDEERDDEARTHLERARDAAATLNDYALAERARTLLDILAPPSSIYRSLDEQSTDMPL
                     SEEPSRPRQYPPLH"
     gene            2791..3504
                     /locus_tag="VFV93_00630"
     CDS             2791..3504
                     /locus_tag="VFV93_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163869.1"
                     /translation="MLLRSAAVVEERVSFCLAGGERCQGSPFLFVDKGRTHLDGRAKM
                     LLALADPVERAAGDAAAAQAVIAELREAVDGRDPDEVLGALHATFATLSRRLLAENRA
                     NAGRRKVFLGLTCVVRCGDDLFIAQVPPGQALIRQDGCLFAFPKLRSWLPVYQPSRTY
                     ELPNPLGLREITEPQVYYTRVVEGDLLAVASSSIAARLEPLEDELRDAAGVDDVQDTL
                     ADLAGRHGLVSGEAGVVFV"
     gene            4072..5484
                     /locus_tag="VFV93_00635"
     CDS             4072..5484
                     /locus_tag="VFV93_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163870.1"
                     /translation="MANQFGSWALRGIVVLELLAGLVLSLTAAVVGVWGVSRRDRPLH
                     GPIDDGMLGLPRLQRWDASYRPRRMQRLRSRFPRMEISRVVIIFCLLIIIAGVGAFIY
                     SRSTEKTILAEQTFESKLAKVAQHRTEALSADDLAAAWAMLIEAKAELEQVAALADDE
                     ADNAAIAVERDAITSQIQHLSKEQPVASVQTVGAVPAALEGTTARLVAGDGRVYLLSD
                     GIYQVDVASKSLVLLLKPGDMVDNVPVETLRAAAWRENRLMVIDARRAYIFDPTTGGW
                     TVEQLGTFDSEGFSDVTAGAAFDFNLYLLARGSGQILKFQAGLYENNPEDWTGGLAQE
                     ELTKAADMAVDGDVWVLLENGKILNFFRSRLEATIAPEIVPPLEKASAIFTTAESSFL
                     YVLSATDGRILRLSREGDLVQQLTTGDKQSMIAGADDLVIDEKSGIAYVLAHDTIYTL
                     RIPAAPGGTETNGTATPQSR"
     gene            complement(5575..5820)
                     /locus_tag="VFV93_00640"
     CDS             complement(5575..5820)
                     /locus_tag="VFV93_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX5163871.1"
                     /translation="MTYVISEPCIGVKDASCVEVCPVDCIHTDDNADQYFIDPDECID
                     CGVCAEVCPVEAIFFEDDLPEQWSKFLLTNRQYFENR"
     gene            complement(6010..>6242)
                     /locus_tag="VFV93_00645"
     CDS             complement(6010..>6242)
                     /locus_tag="VFV93_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5163872.1"
                     /translation="FDNPESLDLSRRPNRHIAFAHGIHFCLGAPLARAEGQIAINTLV
                     QRFPTLAPTSDRVEWQRNHVFRALKSLPVTLQ"
BASE COUNT         1081 a         1832 c         2277 g         1052 t
ORIGIN      
        1 ggtgatcgct gttcagtccg agggaatgcc ggtggtgtac cgctcgttcc acgagcggca
       61 gctgctgtcg ctggaaggtg gcagcacctg ggcggaaggg ctggcgacgc gggtcgcgtt
      121 cgagttgccg tatgcgatga tgcaggaact ggtggacgac gtccagacgg tgagcgagga
      181 ggagatgcgc caggcgatgc tggtcctgct ggagcaggca cacatcgtcg ccgagggcgc
      241 cggtgcggca tcgctggcac tggcgacccg gctggccccg gagttgcgag gacaacgtgt
      301 cggcctcatc gttagtggcg gcaacgtgac tcgcgagaca cttcgccggg cgatgtccga
      361 cgacgggagc tggtaggaac aggcatactc gtgcagccgt gccgcggtgg gtgacggacg
      421 gttgcagtct gggaccgggt ggggggcgcg cagcggatgc tggaacagtt cagccgctgg
      481 cttcggcgtt atcaagggac cgaagccaac ccgatctatc gtctggcggt cgatgtcgcg
      541 accgggcgga tgtcgttcga gcgtgccctt gacgcggcgg aatcgcacag cgtcaacggc
      601 cggctggctg acggggacct ggtcgatctg gaccagcagg tcgagagtga agcgcgcgtc
      661 aatctgcaat tcgcactgat cctggctcgg ctgaacgccg cgacggcgcg ggcaaaaggg
      721 ttcgaaaagg ttctcgttga cctctacctg cggatcgccg aactgctgga agacgagcgg
      781 gacgaggccg agcgcgaata caactaccgt gaggcgctgc acgtcgccaa gcggatctcg
      841 tatgtcgcgg gccagcggcg ggcgctgaac cggctggcgc gaatggcgtt cgaacggggc
      901 gacaccgagg acgcgcgcgg cctcctgctg caacagctcg aagcgggccg cgaggagagt
      961 gacaccggcg acgagatcga gaccgcgatc ctgctggccg atatctcgct ggcggacggc
     1021 gacgtgtcga cggcgcacga cctgtatcac cgggcggcgc gctcggcgcg gcggatcgga
     1081 ttccacaccg gcgccgtcga cgcactgctg cgacaagttg ggattctgcg cgagcggggg
     1141 gatctgcacg cgtcgctaca actcctgcga caggcgagcg agtcggccga tcgaacggtc
     1201 gacacggccc tgcaagccga gattgcgttc cggaccggcg cgctgatgcg cgaactcaac
     1261 atgcccgagg aagcgaccga gcggctgacg atcgcgctgc agcgggcgcg aacgctcggc
     1321 gacatggcga tggaagcccg tagcctgctg atgctggccc agctggaaca gcggatggga
     1381 cgcgacgagc aggcgctgca gcactacgag gcagccgtcg ggctggagcg cgggcttggc
     1441 aatcgcggcg aggtggcgcg agcgttgcag gcgatggggg agatacacct ggcgcaggac
     1501 cggccatcag cggccatcag cgtgctcacg gaagcgcgcg agatggtgct gtactcggat
     1561 gacaccgagc tgctgatgca ggtgcatggg atgctgggca aggcgctggc cgaccagggg
     1621 cgcgagcggg aggcactgga cgcgctccag acggcgctgg cgagcagcag ggcgctggac
     1681 gacccgaccg aggaggcgcg ctggatcatc accgtcgccg aggtgatgct gcgcaccgga
     1741 gagaccgaca acgccggcga gcttgcccag cgcgccgagc agctggcacg cggttcgagc
     1801 aacaacgtgc tgcgggcggc ggtctacagc ctgaacgggc aagtggcgct ggtcgaccgg
     1861 cgcttgcagg acgctgagga cgcgttcgcg tcggcgacgg cggcatcacg agctgccggg
     1921 cagagcggcg catcgctacg ctacctgccg ctgctgtcac ggctggcggc cgaactgggc
     1981 gatatggacg gggcgatccg ctacctggac gaggccgttg ccgaggcgca ggcggtcggt
     2041 gaagacgcgc aggcgtgcat gtttcatggc caggccgcgc ggttgctcgg atcggccgga
     2101 cggatccgcg acgccgagga gcgctaccag cgggcactcg cgctcggacg gagtgcggga
     2161 gatccgcgcc tctccggccg ggcgttgcag ggactggcgg cgatctatga cacgaccggc
     2221 cagctcgaac tggcgatcga acactactac gacgcgctgg acgcagctaa ccgggccggc
     2281 gacgcacgaa cgatcgcctc gacgcatttc aacctcggcg cgctgctggt ggacgaggag
     2341 cgcgacgacg aagcccgcac gcacctggag cgggcccgcg atgcggcggc gacgctgaac
     2401 gactatgcat tggccgagcg ggcacggacg ctgctggaca tcctggcgcc gccgtcatcg
     2461 atctaccgca gcctggatga gcaatcaacc gacatgccac tgagcgaaga gccgtcgcgg
     2521 ccacggcagt acccgccact gcattgattc ctccccacct gtcccaatga cccgacatcc
     2581 catgaacctt cgctaaccgg taggggcgag gcagtgcctc gcccgtgtcg gatcgctcgc
     2641 acgtcaagaa gggcgagtca gtgcctcgcc cctaccggtc ctgatacgaa caacatgcag
     2701 cagcccagtg tacgtacttg atgtacgtac actgggctgc tacgctttgc gacgtaaggc
     2761 gtcaacggta cgtgtgccgg tgggggtgct gtgttattgc ggagcgcggc ggttgtcgag
     2821 gagcgggtct cgttctgtct ggcgggcggc gagcgctgcc aagggtcgcc atttctgttc
     2881 gtcgacaagg gtcgtacgca ccttgatggt cgggcgaaga tgctgcttgc gctggcggat
     2941 ccggtggagc gggcggccgg ggatgcggcg gcggcacagg cggtcatcgc ggagttgcgg
     3001 gaggccgtcg acggtcgcga tccggatgag gtgcttgggg cgctgcacgc gacattcgcg
     3061 acgctgagcc gccggctgct ggccgagaat cgagcgaacg ccggccgacg gaaggtgttc
     3121 ctcggcctga cctgcgtcgt gcgttgtggc gacgatctat tcatcgctca ggtgccgccc
     3181 ggtcaggcac tgatccgaca ggatggctgc ctcttcgcat ttccgaagct gcgtagctgg
     3241 ttgccggtct accagccctc gcggacgtac gaactgccga acccgctggg gctgcgcgag
     3301 atcaccgagc cgcaggtgta ttacacccgg gttgtcgagg gcgatctgct ggcggtcgcc
     3361 tcgtcgtcga ttgcggcccg gctggagccg ctggaggacg aactgcgcga cgcggccggt
     3421 gtcgatgacg tccaggacac gctggcagac ctggcgggcc ggcacggact cgtgagcggc
     3481 gaagcgggcg tcgtcttcgt ctgagaaagg cttcgccccc actgtcgtcc tgagccgcag
     3541 cgaaggacct ctccgcgcta accgcccacg ggtctaacgc ggagaggtcc ttcgctgcgg
     3601 ctcaggacga cagtggggtg ccgatcgtca aattgtgtct ttaggtggcg ctggtgtgac
     3661 ctggcggcac ggttggtccg gctcgggtac tggatttcgg ccaattcccc aaattggtgt
     3721 gctttccccc ttgaccagga tgcaccgaag gtgcagactg agagtgttca acggagattg
     3781 catgactggc tcggtccatc gccggggtat cctcggtctg gccctgccat gcgtgcgaaa
     3841 ccggaacctg gggggattgc gtttccattc tgttgaccca gcgatacacg atcggacacc
     3901 accgccgatg ctgagcggcg gactcgtgat gtggtgaatc agcgcctgcc ggccggaaac
     3961 agggctgggc ggcaggttga gtgtattcgt gccggatggg gccgattcat cgggcgcgat
     4021 actgttatgt gatagcaaac agaccagccc gcccggcctg ggatcgcgcg tttggcgaac
     4081 cagttcggat cgtgggcgtt gagagggatc gtcgtgcttg aactgctggc ggggctcgtc
     4141 ctcagcctta cggcggcagt cgttggtgtc tggggtgtca gccggcggga ccgtccgttg
     4201 cacgggccga tcgacgacgg catgctcgga ttgccgagac tacagcgctg ggatgccagc
     4261 tatcgtccgc gccgcatgca gcgtttgcgc agccggttcc cgcggatgga aatcagccgc
     4321 gtcgtcatca tcttctgctt gctgatcatc atcgccggtg tcggggcgtt catctactcg
     4381 cgctcgaccg agaagacgat cctggccgag cagacgttcg agagcaagct ggccaaggtg
     4441 gcgcagcatc ggaccgaggc actctcggcc gacgatctgg cagccgcctg ggcgatgctg
     4501 atcgaggcga aggcggaact ggagcaggtt gcggcgctgg ccgacgatga ggccgacaac
     4561 gcagccatcg ccgtcgaacg ggatgccatt accagccaga tccagcatct gagcaaggaa
     4621 cagccggtcg caagtgtcca gacggtcgga gccgtcccgg cagcgttgga gggaacgacg
     4681 gcgcggcttg tggccggcga cgggcgcgtc tacctgttgt cggacggcat ctatcaggtt
     4741 gatgttgcca gcaagtcgct ggtgctgcta cttaagcctg gcgacatggt cgacaacgtg
     4801 ccggtcgaga cgctgcgggc cgctgcctgg cgcgagaacc ggctgatggt catcgacgcg
     4861 cgccgggcct acatcttcga cccgacgacc ggcggctgga cggtcgagca gcttgggacg
     4921 ttcgacagcg aggggttctc ggatgtcacg gccggggcgg cgttcgattt caacctctac
     4981 ctgctggcgc gcggcagcgg ccagatcctg aagttccagg cggggttgta tgagaacaat
     5041 cccgaggact ggaccggcgg gctggcgcag gaagagttga cgaaggctgc cgacatggcc
     5101 gtcgatggcg atgtctgggt gctgctggag aacggcaaga tcctgaactt ctttcgctcg
     5161 cgactggagg cgacgatcgc accggagatc gtgccgccac ttgagaaggc cagcgcgatc
     5221 ttcacgacgg ccgagagcag tttcctgtat gtcctgagcg cgacggatgg gcggatcctg
     5281 cggctgtcgc gtgagggcga cctggtccag cagctgacga cgggcgacaa gcagtcgatg
     5341 attgccggtg cggacgatct ggtgatcgac gagaagagcg gcatcgccta cgtcttggca
     5401 cacgacacga tctatacctt gcggattcca gcggcgccgg gcgggacgga gacgaacggg
     5461 acggccacgc cgcagtcgcg gtgacgcggg atgcgtgacg cgtgagagaa gcaggagaac
     5521 gccctcgaca agtgaaccgt cgagggcgtt gacagtttgc gtacgtggtg gagatcagcg
     5581 gttctcgaag tactgccggt tggtgagcag gaacttcgac cactgctcgg ggaggtcgtc
     5641 ttcgaagaag atggcttcga cggggcagac ttcagcgcag acgccgcagt cgatgcattc
     5701 gtcgggatcg atgaagtatt ggtcggcgtt gtcatccgtg tggatgcagt cgaccgggca
     5761 gacttcgaca catgaggcgt ccttgacgcc gatacagggt tcgctgatca catacgtcat
     5821 ccagagatcg cctttctcgg agagttgtcg cgaaatcgct ctatttctcg cggtcgcccg
     5881 ggtcttgccc gacgatcctg atctgcatac ctgaagcgca gtatacaacg ggacgccgat
     5941 gctgggacgt gacaatcgtc ctcacaatgt gtagcggccg ggtgcccggc cgctacacat
     6001 tgcgacttgc tattgcaacg tcaccggcag ggatttgagg gcgcggaaga cgtggttacg
     6061 ttgccattcg acgcggtcgc tggtcggagc gagggtcggg aagcgctgga cgagggtgtt
     6121 gatggcgatc tggccttcgg cgcgggcgag tggtgcgccg aggcagaagt ggatgccatg
     6181 cgcgaacgcg atgtggcggt tcggccgccg gctgaggtcg agcgattcgg ggttgtcgaa
     6241 ct
//
LOCUS       DASUGJ010000027        13702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1670019, whole genome shotgun sequence.
ACCESSION   DASUGJ010000027 DASUGJ010000000
VERSION     DASUGJ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13702
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670019"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /gene="rpsC"
                     /locus_tag="VFV93_00650"
     CDS             <1..557
                     /gene="rpsC"
                     /locus_tag="VFV93_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX5163873.1"
                     /translation="QLTRAGISRVEIERAANRIEVSIHTSKPGIVIGKQGANVEKLRQ
                     MLEAKVGKKVHLKIEEIKVPELDARLVAESIAEQLSRRVSYRRAMKHAVGQAMRRGAK
                     GVKIRLGGRLGGAEMSRSVTETDGRVPRHTLRADIDYGVVHAPTTMGRIGVKVWIYRG
                     DILPERKPQEAPAQPVAEPTPAAD"
     gene            594..1049
                     /gene="rplP"
                     /locus_tag="VFV93_00655"
     CDS             594..1049
                     /gene="rplP"
                     /locus_tag="VFV93_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX5163874.1"
                     /translation="MLMPRRTKHRKVFRGRMQGKAIKGSHVVFGEYGLQSLDPAWIDS
                     RQIEAARRAMTNYIRRGGKVWIRIFPDKPVTQKPAETRMGSGKGNPEYWVAVVRPGRI
                     MFELAGVREDVAMEALRRAAHKLPVQCRIVKRDQVGVAAAEEAHAEAEA"
     gene            1053..1286
                     /gene="rpmC"
                     /locus_tag="VFV93_00660"
     CDS             1053..1286
                     /gene="rpmC"
                     /locus_tag="VFV93_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX5163875.1"
                     /translation="MKADEIRGFTDAQIDEMIDALREEWRQLRFDDAVGRLTNTSRIR
                     HIKRDIARLKTIQTERVMNEEIAALAGAERAGR"
     gene            1314..1622
                     /gene="rpsQ"
                     /locus_tag="VFV93_00665"
     CDS             1314..1622
                     /gene="rpsQ"
                     /locus_tag="VFV93_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX5163876.1"
                     /translation="MAEQTSSAPGAGTTAEVKKTVRVQKVGRVVSDKMQKTVVVAVDY
                     PRRHRLYNKVMTRTSRFKAHDENNDCKVGDIVRIEESRPLSKDKRWVVREIIERADIV
                     "
     gene            1646..2014
                     /gene="rplN"
                     /locus_tag="VFV93_00670"
     CDS             1646..2014
                     /gene="rplN"
                     /locus_tag="VFV93_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX5163877.1"
                     /translation="MIQTQTRLRVADNSGAREVMCIRVLGGSGKKYAGVGDIIIGAVK
                     SAQPGAAVKKGDVVRAVVVRTAAEYGRQDGSHIRFDDNAAVLITPQGNPRGTRIFGPV
                     ARELREKQFMRIVSLAPETL"
     gene            2050..2373
                     /gene="rplX"
                     /locus_tag="VFV93_00675"
     CDS             2050..2373
                     /gene="rplX"
                     /locus_tag="VFV93_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX5163878.1"
                     /translation="MAEKIISGDEVIVLRGRNKGERGRVRQNMPREDKMVIEGINIVR
                     KHIARGRARQAGIVEVEAPVHASKVMLICPSCQEATRVGFRRTDSGDKVRFCKKCDAV
                     IARKA"
     gene            2408..2953
                     /gene="rplE"
                     /locus_tag="VFV93_00680"
     CDS             2408..2953
                     /gene="rplE"
                     /locus_tag="VFV93_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX5163879.1"
                     /translation="MAARLKERYLEEIRPQMREEFSYRNDLEVPKVEKIIVNIGVGEA
                     IANNRALDAAIGDLTIITGQKPVVTRAKKSIAAFRLRAGMPIGAMVTLRGDRMYEFMD
                     RLVNIALPRVRDFRGISPKSFDGRGNYTLGLREQLIFPEIEYDKIDKTRGMEVSIVTS
                     AKSDEEGRRLLGLLGMPFREA"
     gene            3087..3272
                     /locus_tag="VFV93_00685"
     CDS             3087..3272
                     /locus_tag="VFV93_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX5163880.1"
                     /translation="MAKKSLIVKANRTPKYTVRGYNRCKLCGRPRAYIRKFGVCRICF
                     RELASNGKLPGVTKSSW"
     gene            3347..3748
                     /gene="rpsH"
                     /locus_tag="VFV93_00690"
     CDS             3347..3748
                     /gene="rpsH"
                     /locus_tag="VFV93_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX5163881.1"
                     /translation="MSVVSDPIADMLTRIRNAGMARRSETTMPSSKILVEIAKILQQE
                     GYIRGWEVQESKPFNHLIVYLKYASDRRHSIRNIRRVSKPGLRVYAGKDEIPRVRNGL
                     GIAIISTPQGVLTGQEARRRGIGGEVICTVY"
     gene            3776..4321
                     /gene="rplF"
                     /locus_tag="VFV93_00695"
     CDS             3776..4321
                     /gene="rplF"
                     /locus_tag="VFV93_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX5163882.1"
                     /translation="MSRIGLQPITIPSGVDIDIKTGLVAVKGPKGQLQQRVHPEMIFS
                     RDNGTLSVARPNDERMFRQLHGLTRTLVNNMVVGVTTGYRKDLEIQGVGYRAALEGKT
                     LVLNVGFSHQVRVDPPAGIEFIVDTPTRLAVTGIDKQLVGEVAAKLRSVRPPEPYKGK
                     GIRYAGEQIRRKAGKAGKVGK"
     gene            4417..4791
                     /gene="rplR"
                     /locus_tag="VFV93_00700"
     CDS             4417..4791
                     /gene="rplR"
                     /locus_tag="VFV93_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX5163883.1"
                     /translation="MRYHYKSQLDARRRRHLRVRAKVSGTPERPRLNVYRSSAHIYAQ
                     LIDDSTAATLAAASDIEADFAGRLSDSTTKSDRAKAVGQLIGERAKAIGVTTVVFDRG
                     GYKYHGRVKAVADGAREAGLDF"
     gene            4836..5399
                     /gene="rpsE"
                     /locus_tag="VFV93_00705"
     CDS             4836..5399
                     /gene="rpsE"
                     /locus_tag="VFV93_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX5163884.1"
                     /translation="MVDRRRGGRANPDEVGDLEERVVQINRVSKVVKGGRRFAFGAVV
                     VVGDGKGNVGAGIGKAGEVPESIRKGVEAAKKNMISVPMAGRTIPHEIEVKYGASRVL
                     LRPASPGTGVIAGGAVRAVVEAAGITDILGKSLGSNNPVNVVRAALKALSELESGQDV
                     SRRRSKRVESLAERRATELQMAGESTH"
     gene            5399..5620
                     /gene="rpmD"
                     /locus_tag="VFV93_00710"
     CDS             5399..5620
                     /gene="rpmD"
                     /locus_tag="VFV93_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX5163885.1"
                     /translation="MATATLKLTWKKSAIGYPQDQRTTIKSLGFTKLNQTIEKPDTPS
                     IRGMVYKVRHLVHVDGEGQINGSSIHSQS"
     gene            5685..6131
                     /gene="rplO"
                     /locus_tag="VFV93_00715"
     CDS             5685..6131
                     /gene="rplO"
                     /locus_tag="VFV93_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX5163886.1"
                     /translation="MRIDDLRSPDGAFHPKKRVGRGHGSGHVKTSGRGTKGQNSRSGG
                     GVRPGFEGGQNPIYRRMPYKRGFTNNFRTDYQILNLDDIERYGLTGNVTPESMHARGA
                     IDDPKKLVKILGNGEVKSALHIRAHKFSESAKAKIEAAGGTAEVID"
     gene            6131..7429
                     /gene="secY"
                     /locus_tag="VFV93_00720"
     CDS             6131..7429
                     /gene="secY"
                     /locus_tag="VFV93_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX5163887.1"
                     /translation="MLEAVINAFKVADLRRKILFTLGILIIFRFIATIATPGVDRQAL
                     DDLLDNNQLLGMLNLFSGSTLSNFSIVAMGVYPYITASIIMQLLTPIIPRLNELSQEG
                     QQGRNRINQYTHWITVPIAFLQAYGQCILMQQSGVLTDFGLFDSDTWLNSLAILITLV
                     TGTILLVWLGELITENGIGNGISIIIFGGIVASLPSTVGNLIYGGSTTDNLIGTLTFV
                     ALGLATIVGIVLINEGMRRIPVHHAKRVRRGRTYGGGSTHIPLKVNSAGMIPLIFAVS
                     IMVFPGTIGNFMTGSERAWVRNLGTDMARFFDATTVLYQVIYFVMVVAFTFFYTMVVF
                     QQQNISENLQRQGAFIPGIRPGLNTKRYLEGVLTRITLVGALFLGVVAVLPFIAAEIT
                     GVQTLFLGSTSLLIVVGVAIDTMRQLEAQLMMRNYEGFIQ"
     gene            7501..8199
                     /locus_tag="VFV93_00725"
     CDS             7501..8199
                     /locus_tag="VFV93_00725"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEX5163888.1"
                     /translation="MSQQPMRHVVFVGPQGAGKGTQAARVAPRLGLIHLATGDLFREL
                     MASESPLASEVRAFYDAGNLVPDELTARVLFAALDRGAQSAGVTGALFDGFPRNSAQA
                     DVLDQQIDSRGETLAAVVHIAVPRVVLMDRLTGRLTCRNCGRTYHVRFNPPAREGVCD
                     ACGGELYVRSDDTEEAVARRLEIYYQQTEPLLNRWGQRGIVHEIDGNRDIDAVTEAII
                     ATLATAGDFNESQG"
     gene            8211..8966
                     /gene="map"
                     /locus_tag="VFV93_00730"
     CDS             8211..8966
                     /gene="map"
                     /locus_tag="VFV93_00730"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX5163889.1"
                     /translation="MLKSPADIERMQAAGAVIASVHERLRGLVQPGITTRELDAAGRE
                     MIEAAGGTPSFLHYRGYPASVCTSVNDEILHGIPGDRVLQEGDIISLDLGVYLDGFHG
                     DAAVTHPVGTVDPRLIQLIETTEECFWVGFRALKLGGRLGDAASVVQELAESRGYGVV
                     REYAGHGVGRRIHEDPSVPNYGTAGSGTLLRRGMTFALEPMLTLGGYQTRLLPDNWTV
                     ATADGSPAAHFEHTIAMTEDGPIILTGMPANVI"
     gene            9025..9246
                     /gene="infA"
                     /locus_tag="VFV93_00735"
     CDS             9025..9246
                     /gene="infA"
                     /locus_tag="VFV93_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX5163890.1"
                     /translation="MPKKDVIEVEGKVTEPLPNAMFRVELDNGHEVLAHISGKLRMNF
                     IRILPGDRVRVELSPYDLTRGRITYRLRT"
     gene            9345..9458
                     /gene="rpmJ"
                     /locus_tag="VFV93_00740"
     CDS             9345..9458
                     /gene="rpmJ"
                     /locus_tag="VFV93_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX5163891.1"
                     /translation="MKVSASVKVRCERCKVIRRRGVVRVICSNPKHKQRQG"
     gene            9470..9847
                     /gene="rpsM"
                     /locus_tag="VFV93_00745"
     CDS             9470..9847
                     /gene="rpsM"
                     /locus_tag="VFV93_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX5163892.1"
                     /translation="MARIAGVDIPRDKRVEYALPYIYGIGLATSQQILARTGIDPSTR
                     VRDLTDDEVGRLRDIIEKEMRVEGDLRREVALNIKRLIDIGSYRGLRHRRGMPLRGQR
                     TKTNARTRRGPKRAIGLKKKATR"
     gene            9996..10406
                     /gene="rpsK"
                     /locus_tag="VFV93_00750"
     CDS             9996..10406
                     /gene="rpsK"
                     /locus_tag="VFV93_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX5163893.1"
                     /translation="MADRRRGAARGKARVRRRDRKNIPRGKAYISSTFNNTIVNLTDP
                     AGNTIAWASAGASGFKGSRKSTPYAAQVTAESAARKAMEHGLRQVEVFVKGPGAGREM
                     AIRALAATGVQVLAINDVTPIPHNGCRPPKRRRV"
     gene            10522..11166
                     /gene="rpsD"
                     /locus_tag="VFV93_00755"
     CDS             10522..11166
                     /gene="rpsD"
                     /locus_tag="VFV93_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX5163894.1"
                     /translation="MARYTGPVCRLCRREGMKLYLKGERCLGPKCPIVQRNPQRNFPP
                     GMHGQKRTRRPSEYGLQWREKQKVRRFYGVMEKQFSKHFEDATRQPGVTGENLLRILE
                     MRLDNVVYRLGFADSRKQARQIVRHGHFYVNGRKTDIPSYQARPGDEISVRPQSRDKE
                     YFKVVAEVLRTRQVPGWLTLDPVAMSGRVVTPPTRDQIDMPPINEQLVIEFYSR"
     gene            11259..12272
                     /locus_tag="VFV93_00760"
     CDS             11259..12272
                     /locus_tag="VFV93_00760"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX5163895.1"
                     /translation="MQELAQFKVDLTTSGHNYGRFTIEPLEPGYGITMGNSLRRILLR
                     SLPGAAIVRARIADVWHEFATIDGVREDVTEIVLNLKRIRIRSVGPLHETRAHLYHEG
                     KGVVTAGDIDWPSDIEVVNPELVIASVDDEKATIEMDLVVAAGRGYLPAEAQDSFAIG
                     EIPLDALFTPIEKVNYVVEHTRVGQMTDFDRLIIEIQTDGTIEPEDALSQAANILVDY
                     AQKIAGFNREDGAGVEEAARTPSEADSRALADLGLSPRVLNALRSRQIEKVGQVMTME
                     RDQLLSIRNFGPRSLRELTDALTAHGYDIPATLIGAGGGDADDADAGESDAGQEMEEE
                     LSS"
     gene            12269..12622
                     /gene="rplQ"
                     /locus_tag="VFV93_00765"
     CDS             12269..12622
                     /gene="rplQ"
                     /locus_tag="VFV93_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX5163896.1"
                     /translation="MRHQVAGKKFGRNSGQRRALFKGLAVSLILNERITTTETKAKTI
                     RPIVEKLVTMAREDTEHHRRLVMARLGNELATTKLFEVIAPRYDGQGGGYTRIYKIGV
                     RRGDAAPVSMIEFVE"
     gene            12661..13485
                     /gene="truA"
                     /locus_tag="VFV93_00770"
     CDS             12661..13485
                     /gene="truA"
                     /locus_tag="VFV93_00770"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX5163897.1"
                     /translation="MRRVRITLGYDGTEFFGSQSQPGRRTVQDELERLLQRLSPDSGR
                     TAFAGRTDRGVHAVGQVVSVDVDWRDSDRALRDALNSLAPVDLAFVDVATVANTFHAR
                     YDARWREYRYRIVVAAVPPVFERRYCWWRHTAIDADLAADGCRRVVGEHAFGTFAGSG
                     KSRSLPAGKLVRTVRDCDWRATDGGSRHELRIVASGFLPQMVRNITGAIVQVGSGRRE
                     PGWIDELIAANDRRVLGDAAPPEGLTLWRVSYPGDDNEQRDEASARQWPGEGYGEV"
     gene            13485..>13702
                     /gene="rplM"
                     /locus_tag="VFV93_00775"
     CDS             13485..>13702
                     /gene="rplM"
                     /locus_tag="VFV93_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX5163898.1"
                     /translation="MAAIRTYVPKATEIERRWYVVDAEGQTLGRLATQIATVLRGKHK
                     PTFTPHLDVGDYVIVINAEKVHVTGNKE"
BASE COUNT         2618 a         4240 c         4593 g         2251 t
ORIGIN      
        1 atcagctgac gcgggcgggc atctcgcgcg tcgagatcga gcgggccgcg aatcggatcg
       61 aagtctcgat tcacacctca aagccgggca tcgtgatcgg caagcagggc gcgaacgtcg
      121 agaagctgcg ccagatgctc gaggcgaagg tcggcaagaa ggtccacctc aagatcgaag
      181 agatcaaggt gccggaactg gatgcccggc tggtggccga gtcgatcgcc gagcagctca
      241 gccgccgcgt gtcctaccgc cgcgcgatga agcacgccgt cggccaggcg atgcggcgcg
      301 gcgccaaggg cgtcaagatt cgcctcggcg gccgcctcgg tggcgccgag atgtcgcgta
      361 gcgtgaccga gacggacggc cgcgtgccgc gtcacacact gcgggccgat atcgattacg
      421 gcgttgtcca cgctccgacg acgatgggcc ggatcggtgt caaggtctgg atctatcgcg
      481 gcgacatcct gccggagcgc aagccgcaag aggcaccggc ccagccggtc gccgagccga
      541 caccggccgc cgactagggt cgccgcaagg cgggtgaggg gagtacgaca accatgttga
      601 tgccacgacg caccaagcac cgcaaggtgt tccggggcag gatgcagggc aaggcgatca
      661 aaggctcgca cgtcgtcttt ggcgagtacg gcctgcaatc gctcgatccg gcctggatcg
      721 acagccgcca gatcgaggcc gcccgccgcg cgatgacgaa ctacattcgt cgtggcggca
      781 aggtctggat ccgcatcttt ccggacaagc cggtgacgca gaagccggcc gagacccgga
      841 tgggctccgg caagggcaac ccggagtact gggtcgccgt cgtccggccg ggccggatca
      901 tgttcgagct cgcgggtgtc cgcgaggatg tcgcgatgga ggcgctgcga cgcgcggcgc
      961 acaagctccc ggtacagtgc cggatcgtca agcgcgatca ggtcggagtc gctgccgccg
     1021 aggaagcaca cgcggaggcg gaggcgtaac agatgaaggc tgacgagatt cgcggcttca
     1081 ccgacgcaca aatcgacgag atgattgacg cgctccgcga ggagtggcgc cagcttcgct
     1141 tcgacgatgc ggttggccgg ctgacgaata cgagccggat caggcatatc aagcgcgata
     1201 tcgcccggct caagacgatt cagaccgagc gggtcatgaa cgaggagatc gcggcgctgg
     1261 ccggcgcaga gcgcgccggt cgctaacggc gacctgacgg aggaacgtag tagatggcag
     1321 aacagacaag ctccgcgcca ggcgccggga cgacggccga ggtcaaaaag accgtccgcg
     1381 tccagaaggt cggccgggtc gtgagcgaca agatgcagaa gacggtcgtc gtcgcggttg
     1441 actacccgcg ccgccaccgg ctgtacaaca aggtgatgac tcggacgagc cgcttcaagg
     1501 cgcacgacga gaacaacgac tgcaaggtcg gcgacatcgt gcggattgaa gagtcccggc
     1561 cgctgtcgaa ggacaagcgc tgggttgtcc gcgagatcat cgagcgcgcg gacatcgttt
     1621 aggaacgtcg gggagggcag aggtcgtgat tcagacacag actcgcttgc gcgtcgccga
     1681 caattccggc gcgcgcgagg tgatgtgcat ccgggtcctg ggtggctcgg gcaagaaata
     1741 cgccggcgtc ggcgacatca tcatcggcgc cgtcaagtcg gcccagccgg gtgcggcagt
     1801 caagaagggc gatgttgtcc gggcggtcgt cgtccgaacg gccgccgaat atggccggca
     1861 ggacggctca cacatccggt tcgacgacaa tgcagctgtc ctgatcacgc cgcagggtaa
     1921 cccgcgcggg acgcgtatct tcggcccggt cgcccgcgaa cttcgcgaga agcagttcat
     1981 gcgcatcgtc tcattggctc cggaaacgct gtagtgacat aatatctgga tcgtacggga
     2041 gcgagtagca tggctgagaa gatcatttcg ggcgatgagg tcatcgtcct gcggggacgc
     2101 aacaagggtg agcgcgggcg agttcggcag aacatgccgc gcgaggacaa gatggtcatt
     2161 gagggcatca acatcgtccg caagcacatc gcacgcggcc gggcgcgcca ggcaggcatc
     2221 gtcgaggtcg aagcgccggt ccacgcgtcg aaggtgatgc tgatctgccc gtcgtgccag
     2281 gaggcgacgc gggtcggctt ccggcgcacc gacagtggcg acaaggtccg cttctgcaag
     2341 aagtgtgacg ccgtcatcgc ccgcaaggcg tagccgcggg gctgcacagg atacgtgagg
     2401 gagtaacgtg gctgcacggt tgaaggaacg ctatcttgag gagatccgcc cgcagatgcg
     2461 ggaggagttc tcgtatcgca acgacctcga ggtgccaaag gtcgagaaga ttatcgtcaa
     2521 catcggcgtc ggcgaggcga tcgcgaacaa ccgtgcgctc gatgctgcca ttggcgacct
     2581 gacgatcatc accggccaga agccggtcgt gacgcgtgcc aagaagtcga tcgccgcctt
     2641 ccgcttgcgg gccggcatgc cgatcggtgc gatggtgacg ctgcgcggtg accggatgta
     2701 cgagttcatg gaccggctgg tcaacatcgc gttgccgcgc gtccgtgact tccgcggcat
     2761 ttcgccgaag tcgttcgatg gccgtggcaa ctacacgctc ggcctgcgcg agcagttgat
     2821 cttcccggag atcgagtacg acaagatcga caagacgcgc ggcatggagg tgtcgatcgt
     2881 cacctcggcc aagtcggatg aagaaggccg ccggctgctc ggcctgcttg gaatgccgtt
     2941 ccgcgaggcc tgatacgttc aggcgacggt gagccacggt ggggcacgtc ccacaccacg
     3001 acgcagtcac aggaatcgag tgggaccgtt gaagcccatc ggcccgtctg ggagttggga
     3061 cggaagagga agcagaaagc aacgagatgg cgaagaagtc actgattgtc aaagcgaacc
     3121 ggacgccgaa gtacaccgtg cgtggctaca accgctgcaa gctgtgtggc cgtccgcgcg
     3181 cctatatccg caagttcggc gtctgccgga tttgcttccg cgaactggcg tcgaacggca
     3241 agctgccggg cgtgacgaag tcaagctggt agttgttgtc actgaggcat accggcacac
     3301 cgtcgcccgg tcggcgacgc gaagaacgtg aggtacggag cattacatga gtgtcgtgag
     3361 cgatccaatc gccgatatgc tgactcgcat ccgcaacgcg ggtatggcgc ggcgctccga
     3421 gacgacgatg ccgtcgtcga agatcctggt cgagattgcc aagatcctcc agcaggaggg
     3481 ctacattcgc ggctgggaag ttcaggagtc gaagccgttt aatcacctga ttgtctacct
     3541 gaagtacgcg agcgatcggc ggcactcaat ccgcaacatc cgccgcgtct cgaagccggg
     3601 cctgcgtgtc tatgccggta aggacgagat tccgcgcgtc cgcaacggtc tcggcatcgc
     3661 gatcatcagc acgccgcaag gtgtgctgac cgggcaggaa gcccgccgcc gtgggatcgg
     3721 tggcgaggtt atctgcaccg tttactagca gacgacggga gaggaggaag ccaccatgtc
     3781 ccgaattggc ctgcaaccga ttacgatccc gtcaggcgtc gatatcgata tcaagactgg
     3841 cctggtcgcc gtcaaggggc cgaagggcca gcttcagcag cgcgtccacc cggagatgat
     3901 cttctcgcgt gacaacggca cgctgtccgt cgcgcggccg aacgacgagc gcatgttccg
     3961 ccagctgcac gggctgacgc ggacgctggt caacaacatg gtcgtcggcg tgacgaccgg
     4021 ctaccgcaag gatctggaga tccagggcgt cggctatcgc gccgcgctgg agggcaagac
     4081 gctcgttctg aacgtcggct tctcgcatca ggtgcgggtc gatccgccgg ccggcatcga
     4141 gttcattgtc gacacgccaa cccgcctggc ggtgaccggc atcgacaagc agctcgtcgg
     4201 cgaggtcgcc gccaagctgc gctcggtccg cccgcccgaa ccgtacaagg gcaagggcat
     4261 ccgctacgcc ggcgagcaga ttcgccgcaa ggccgggaag gccggcaagg tcggcaagta
     4321 gccggcgggc cgcgacatgc ggccaccaat ttggcgttga tgtgctggtg gccgggatag
     4381 aatccggttg cccgaagcac cagggagcag cgaacgatgc gatatcacta caagtcccag
     4441 ctcgacgcgc gacggcgacg tcacttgcgt gtccgggcca aggtgagcgg cacgccggag
     4501 cgaccgcgcc tgaacgtcta ccgcagctcg gcgcatatct acgcccagct tatcgacgac
     4561 agcaccgccg cgacgctggc ggcggcctcc gacatcgagg cggatttcgc cggccggctg
     4621 tcggacagca cgaccaagag cgaccgcgcc aaggcggtcg ggcagttgat cggcgagcgc
     4681 gccaaggcga taggtgttac aactgtcgtc tttgaccggg gcggctacaa gtatcacggt
     4741 cgcgtcaagg cagttgccga tggcgctcgt gaggccgggc tcgacttcta atcgcaggac
     4801 gcggcagccg ccgccggagt gaggtacgaa tactgatggt agatcgacgc aggggaggac
     4861 gggcgaatcc ggacgaggtc ggcgacctcg aggagcgcgt tgtccagatc aatcgcgtgt
     4921 cgaaggtcgt caagggcggc cggcgcttcg cctttggcgc ggtcgtcgtc gtcggcgatg
     4981 gcaagggcaa cgtcggcgcc gggattggca aggccggcga ggtgccggag tcgatccgca
     5041 agggtgtcga ggcggccaag aagaacatga tcagcgtgcc gatggccggc cggacgatcc
     5101 cgcacgagat cgaggtcaag tacggcgcgt cgcgggtgct gctgcggccg gcctcgccag
     5161 ggaccggtgt gatcgccggt ggcgcggtcc gcgccgtcgt cgaggcggcc ggcatcaccg
     5221 acatcctcgg caagtcgctc ggttcgaaca atcctgtcaa cgtcgttcgg gcggcgctca
     5281 aggcgctcag cgaacttgag agcggtcagg atgtgtcgcg ccgtcgcagc aagcgcgtcg
     5341 agtcgctcgc cgagcgccgc gccaccgagc tgcagatggc aggggagtca acgcactgat
     5401 ggctactgca actctgaagc tgacctggaa gaagagcgcc atcggctatc cccaggacca
     5461 gcggacaacg atcaagtcac tcgggttcac gaagctgaac cagacgatcg agaagccgga
     5521 tacgccgtcc attcgcggga tggtctacaa ggtccgccac ctcgtccatg tcgacgggga
     5581 aggccagatc aacggctcct cgatccactc gcagagctag tcgtcgcgga gcgtatcgac
     5641 gaatcagccg ggacactttg ccccggcatg agggacggaa actgatgaga atcgacgatt
     5701 tgcgttcgcc tgatggcgcg ttccacccga agaagcgggt tggacgcggc cacggctcgg
     5761 gacacgtcaa gacatccggc cggggcacca agggtcagaa ctctcgctcg ggcggcggcg
     5821 tccggccggg cttcgagggc ggccagaacc cgatctaccg gcgtatgccg tacaagcgcg
     5881 ggttcaccaa caacttccgc accgactacc agatcctgaa cctggacgac atcgagcgct
     5941 acggcctgac cggtaacgtc acgccggagt cgatgcacgc gcgcggcgcg atcgacgatc
     6001 cgaagaaact cgtcaagatc ctcggcaacg gcgaggtgaa gagcgcgctc catattcggg
     6061 ctcacaagtt ctctgagtcg gccaaagcca agatcgaggc ggccggcggg acggcggagg
     6121 tcatcgactg atgctggaag cggtgatcaa cgcgtttaaa gttgccgacc tgcgccgcaa
     6181 gatcctgttt acgctcggca tcctgatcat cttccgcttc atcgcgacga tcgcgacgcc
     6241 gggagtcgat cgccaggcgc ttgacgacct gctcgacaac aaccagttgc tgggcatgct
     6301 gaacctgttc tccggttcga cgctgtccaa cttctcgatc gtcgcgatgg gggtctaccc
     6361 gtacatcacg gcgtcgatca tcatgcagct gctgacgccg atcatcccgc gactgaacga
     6421 gttgtcgcag gaagggcagc agggacgtaa ccggatcaac cagtacaccc actggatcac
     6481 ggtcccgatc gcgttcctgc aagcctatgg ccagtgcatc ctgatgcagc aatcgggtgt
     6541 gctcaccgac ttcggtctgt tcgactcgga tacctggctg aactcgctgg cgatcctcat
     6601 cacgctcgtg accggcacga ttctgctggt ctggctcggt gagctgatta ccgagaacgg
     6661 catcggcaat ggcatctcga tcatcatctt cggcggtatc gtcgccagcc tgccgagcac
     6721 ggtcgggaac ctgatctatg gcggctcgac gaccgacaac ctgatcggga cgttgacatt
     6781 cgtggcgctg ggcctggcga cgatcgtcgg tatcgtcctc atcaacgagg gcatgcggcg
     6841 catcccggtc caccatgcca agcgggtccg gcgcggacgc acctacggcg gcggctcgac
     6901 gcacattccg ctgaaggtca actcggccgg catgatcccg ctgatcttcg ccgtctcgat
     6961 catggtcttc cccggtacga tcgggaactt catgaccggc tcggaacgag cctgggtgcg
     7021 caatctcggc accgacatgg cgcgcttctt cgatgcgacc acggtgctgt accaggtgat
     7081 ctacttcgtg atggttgtgg cgttcacctt cttctacacg atggtcgtct tccagcagca
     7141 gaacatctcc gagaacctgc aacgccaggg cgcgttcatc cccggtatcc ggcccggctt
     7201 gaacaccaag cgctatctcg aaggtgtcct gacgcgcatc acgctggtcg gcgcgctctt
     7261 tctcggcgtg gtcgcggttc tgccgttcat cgccgccgag atcaccggcg tccagacgtt
     7321 gttcctcggg tcaacctcat tgctgatcgt cgtcggtgtc gccatcgaca cgatgcgcca
     7381 gctcgaagcg cagctgatga tgcgcaacta cgaaggcttc atccagtaac ggatgctgtc
     7441 ctggacgggg cgaggcgctg attgccgcgc cccgttaccc gcaaaggatc gatacacgaa
     7501 gtgagtcagc aaccaatgcg ccatgtcgtc tttgtcggcc cgcagggtgc cggtaagggc
     7561 acgcaggcgg cccgcgtcgc accgcgactg gggctgatcc atctcgcaac cggcgacctg
     7621 ttccgcgagt tgatggccag cgaatcgccg ctcgccagcg aggtgcgtgc gttctacgac
     7681 gccggcaatc tcgtacccga tgagttgacc gcccgcgtcc tgttcgcggc gcttgatcgc
     7741 ggcgcacagt ccgccggcgt gaccggagct ctcttcgacg gcttcccgcg caacagcgcc
     7801 caggccgatg tcctcgacca gcagatcgat tcgcgcggcg agacgctggc ggcggtcgtc
     7861 cacatcgccg tccccagggt ggtcctgatg gatcggctga ccggtcgcct gacctgccgc
     7921 aactgtggcc ggacctacca tgtgcgtttc aatccgcccg ctcgggaggg cgtctgcgac
     7981 gcctgtggcg gcgagctcta cgtccgctcc gacgataccg aggaagcggt ggcgcgtcgg
     8041 ctggagatct actaccagca gaccgaaccg ttgctgaacc gctggggtca gcgcggcatt
     8101 gtgcacgaga tcgacggcaa ccgcgacatc gacgctgtca ccgaagccat catcgcgacg
     8161 ctggcgacgg cgggcgattt caacgagagc cagggatagc gttgccggtt gtgctgaagt
     8221 ctcccgccga tatcgaacgg atgcaggccg ccggagcggt catcgcgtcg gttcatgagc
     8281 ggctgcgcgg actggtccaa cccggcatca cgactcgcga gctcgacgcg gccgggcgcg
     8341 agatgatcga agccgccggt ggcacgccct cgttcctcca ctaccgtggc tacccggcca
     8401 gcgtgtgtac ctccgtcaac gacgagatcc tacacggcat cccgggcgac cgcgtccttc
     8461 aggaaggcga catcatctcg ctcgatctcg gtgtctacct ggatggcttt cacggtgacg
     8521 cagccgtgac acatccggtc gggacggttg atccgcgcct gatccagctg atcgagacga
     8581 ccgaagagtg cttctgggtc ggcttccggg cgctgaagct cggcggaagg ctcggagatg
     8641 ctgcgtcggt cgtccaggag ctggccgaat cgcgtgggta cggcgtcgtg cgtgagtacg
     8701 ccggccatgg cgtcggccgc cggattcacg aggatcccag cgtcccgaac tacggcacgg
     8761 ccggctccgg gacactgctg cggcggggca tgaccttcgc acttgagcca atgctgacgc
     8821 tgggcggcta ccagacgcgg ctgttgccgg ataactggac ggtcgcgacg gcggatggct
     8881 cgccggcggc ccatttcgag catacgatcg cgatgaccga ggacgggccg atcatcttga
     8941 ccggaatgcc cgcgaacgtg atataatcgg tgattgtcgc gcaagcttcg acttgcgcta
     9001 tttgcgtgtg atgacgggga gtctgtgcca aagaaggacg taatcgaagt cgagggcaag
     9061 gtgaccgagc cgcttcccaa cgcgatgttt cgtgtggagc tggacaacgg ccatgaggtt
     9121 ctcgcgcata tttccggcaa attgcggatg aacttcatcc ggattctgcc gggtgatcgt
     9181 gtcagagtgg agctctcgcc gtacgacctg acacgaggac ggatcaccta ccggctacgg
     9241 acgtagtccc tggccgaatt tctggtggtc acgattgtgc tggcgctgac tgtggcgcta
     9301 ctcgcgcttc gttgttgccg gcgggggatg ggagagagga cgagatgaag gtttctgcat
     9361 cagtcaaggt gcgctgtgag cgctgcaagg tcatccggcg gcgcggcgtc gtgcgcgtca
     9421 tctgctcgaa tccgaagcac aagcagcgac agggctagga gcaacaacta tggcacgaat
     9481 cgcgggtgtt gacattccac gcgacaagcg cgtcgagtac gcgctaccgt acatctacgg
     9541 gatcggcctg gcaaccagcc agcagattct ggcgcgaacc gggatcgacc cgagcacccg
     9601 cgttcgtgat ctgaccgacg acgaagttgg ccgcctgcgc gacatcatcg agaaggagat
     9661 gcgcgtcgaa ggtgacctgc gtcgtgaagt tgcactcaac atcaagcgtc tgatcgacat
     9721 cggctcctac cgcggccttc gccaccgacg cggtatgccg ctgcgcggcc agcgcacgaa
     9781 gacgaacgcc cggacccggc gtggtccgaa gcgggcgatt ggcctgaaga agaaggccac
     9841 tcgctagacg tagttgttca tgtcgtccac gctgtcgacc tggctgccgg gtatcctccc
     9901 cggcgcggct cagccagacc ggcgcgagtg gaacgacgtc acggttcgaa ccgacgacag
     9961 acactggtag acggggtaca ggaggaagca gcagcatggc ggatcgacga cggggtgcgg
    10021 cacgcggcaa ggcgcgtgtc cgaaggcgcg atcgaaagaa cattccgcgc gggaaagcct
    10081 atatctcatc gacgttcaac aacacgattg tcaacctgac cgatcccgcc ggtaacacga
    10141 tcgcctgggc aagcgccggc gcgtcgggtt tcaagggatc gcgcaagagc accccgtacg
    10201 ccgcgcaggt gacggccgag agtgccgccc gcaaggcgat ggagcacggt ttgcgccagg
    10261 tcgaggtctt cgtcaagggg ccgggtgccg gccgcgagat ggcgatccgc gcactggccg
    10321 cgaccggtgt ccaggtgctg gcgatcaatg atgtcacacc gattccgcac aacggctgtc
    10381 gcccaccaaa gcgtcgccgc gtctagcagc cgatcactca tattgccggc gcttcattcg
    10441 gagcgccccg tctcagcatc aacgacggtc tgctggcagc gcgtgagccg cgcggcaggc
    10501 tgaggagggt aagcaaggtt catggcgagg tacacgggtc ccgtttgccg gctctgccgc
    10561 cgcgaaggga tgaagctcta tttgaagggt gagcgctgcc tcgggccgaa gtgcccgatc
    10621 gtccagcgca acccgcagcg caacttccca ccgggcatgc acggccagaa gcgcacgcgt
    10681 cggccgtcgg agtacggcct ccagtggcgc gagaagcaga aggtccgccg gttctacggc
    10741 gtgatggaga agcagttcag caagcacttc gaggatgcca cccggcagcc cggcgtgacc
    10801 ggcgaaaatc tgctgcgcat ccttgagatg cggctcgaca acgtcgtcta tcgtctcggc
    10861 ttcgccgatt cgcgcaagca ggcacgccag atcgtgcggc acggccactt ctacgtcaac
    10921 ggccgcaaga ccgacattcc gtcctaccag gcacgcccag gcgacgagat ctccgtccgg
    10981 ccgcagagcc gcgacaagga gtacttcaaa gtggtcgccg aagtgctgcg tacgcggcag
    11041 gttccgggct ggctgaccct agacccggtc gcgatgtccg gtcgggtggt gacgccaccg
    11101 acgcgcgacc agatcgacat gccgccgatc aacgagcagt tggtcatcga gttctacagc
    11161 cggtaggtgc catagcaatt gcattcaacc cagatggcac ggcgctgcca gatgcatcgg
    11221 atcggtgcgc aacggctact cggaggaggc ggatcagctt gcaggaacta gcacaattca
    11281 aggtcgatct tacaaccagc gggcacaact acgggcgatt cacgatcgag ccgcttgagc
    11341 cgggctacgg catcacgatg gggaactccc tgcgtcgtat cctgctgcgc tcgcttcctg
    11401 gcgcggccat cgtccgggcg cggatcgccg atgtctggca cgagtttgcg acgattgatg
    11461 gcgttcgcga ggacgtcacc gagatcgttc tcaacctcaa gcgcatccgc atccgctccg
    11521 tcggcccgct gcacgaaaca cgcgcacacc tctaccatga gggcaagggc gtggtgacgg
    11581 ccggcgacat cgactggccg tccgacatcg aggtggtcaa ccctgaactg gtgatcgcct
    11641 cggttgacga cgagaaggcg acgatcgaga tggacctcgt cgtcgcggcc ggtcgtggct
    11701 atctgccggc tgaggcgcag gactcgttcg cgatcggcga gatcccgctc gacgcgctct
    11761 tcacgccgat cgagaaggtc aactacgtcg tcgagcacac ccgcgtcggc caaatgaccg
    11821 acttcgaccg gctcatcatc gagatccaga ccgacggcac gatcgagccg gaggatgcgc
    11881 tgtcgcaggc agccaatatt ctcgtcgact atgcccagaa gatcgccggc ttcaaccgtg
    11941 aggatggcgc cggcgttgaa gaggctgccc gcacgccgtc cgaggcggat tcgcgcgcgc
    12001 tggccgatct cggcctctcg ccgcgtgtgc tgaacgcgct gcgcagccgt cagatcgaga
    12061 aagtcggtca ggtaatgacg atggagcgcg accagttgct ctccattcgc aacttcggtc
    12121 cccgctcact gcgcgagctg accgacgcgc tgaccgccca cggctacgac atcccggcga
    12181 cgctgatcgg cgccggtggt ggtgatgctg atgacgccga cgccggcgaa tccgatgccg
    12241 gccaggagat ggaagaggag ttgagctcgt gaggcaccag gtagcgggca agaagttcgg
    12301 ccggaatagc ggccagcggc gcgccctgtt caagggcctc gccgtctcac tgattctcaa
    12361 tgagcgcatc acgacgacgg agacgaaggc caagaccatt cgtccgatcg tcgaaaagct
    12421 ggtcacgatg gcgcgggaag acaccgagca ccatcggcgg ctggtgatgg cgcggctcgg
    12481 taacgagctg gcgaccacca agctgttcga ggtgatcgcg ccccgctacg acggccaggg
    12541 tggcggctac acgcggatct acaagatcgg cgtgcggcgc ggcgatgcgg ctccagtctc
    12601 gatgatcgag ttcgtcgagt aggatcgggg acgcgagaca gtggcgggtc gaacaggctc
    12661 atgcggcgcg tgcgaatcac gcttggttac gacgggaccg aattcttcgg ttcgcagtcg
    12721 cagccgggcc gccgcacggt ccaggatgag ctggaacggc ttctccagcg cctctcgccc
    12781 gattcgggac ggacagcgtt tgccggacgg accgaccggg gcgtccacgc cgtcggccag
    12841 gtcgtctcgg tcgatgtcga ctggcgggac agcgatcgcg ccctgcgcga cgcactcaac
    12901 tcgctggccc cggtcgatct ggcgtttgtc gacgtcgcga cggtcgcgaa cacgttccac
    12961 gcgcgctacg atgcgcgctg gcgtgaatat cgctatcgga tcgtcgtcgc ggctgtaccg
    13021 ccggtgtttg agcggcgata ttgctggtgg cggcacacag ccatcgacgc cgatctcgcg
    13081 gctgacggat gccgacgcgt cgttggcgag cacgcgttcg ggacattcgc cggcagcggt
    13141 aagagccgat cgctcccggc cggcaagctc gtccgaacgg ttcgcgactg cgactggcga
    13201 gcaacggacg gcggcagccg gcacgaacta cggatcgtcg cgagcggttt cctgccgcag
    13261 atggtccgca acatcaccgg tgcgatcgtt caggttggtt ccggccggcg tgagccgggc
    13321 tggatcgacg aattgattgc ggcgaacgac cgccgggtgc tgggcgacgc ggctccgccc
    13381 gagggactga cgctctggcg ggtaagctac ccgggcgatg acaacgaaca acgagacgaa
    13441 gcaagtgccc ggcagtggcc tggcgaggga tacggggaag tgtaatggca gcaatcagga
    13501 catacgttcc aaaggcgacc gagatcgagc gccgctggta tgtcgtcgac gccgaggggc
    13561 aaacactcgg ccggctggcg actcagatcg caaccgtgct gcgcggtaag cacaagccga
    13621 cgttcacgcc gcaccttgat gtcggtgact acgtcatcgt catcaacgcc gagaaggtcc
    13681 acgtcacggg caacaaggaa ga
//
LOCUS       DASUGJ010000028         9135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1670090, whole genome shotgun sequence.
ACCESSION   DASUGJ010000028 DASUGJ010000000
VERSION     DASUGJ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670090"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFV93_00780"
     CDS             complement(<1..305)
                     /locus_tag="VFV93_00780"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX5163899.1"
                     /translation="MSQPRRTLTTIDAHAAGEPLRLITSGVPPLRGATILDLRRELLE
                     QHDDIRRALMWEPRGHADMYGAILTPPVTAEADYGILFMHNEGYSTMCGHGIIAVTT"
     gene            complement(364..1560)
                     /locus_tag="VFV93_00785"
     CDS             complement(364..1560)
                     /locus_tag="VFV93_00785"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5163900.1"
                     /translation="MLRVRARVSGALSDSSVLVTTSVVHLTNDACFALLYPLLPFIAE
                     DLNLSYTQTGLLKATFSGASSVLQVPAGIAGGRYGEMLILLLGNLWVGLGLVGMALTG
                     TFVLLLAVAFLTGIGGNAQHPLAASIVSNHVRREQLATSMGTLNFAGDLGKLIGPFVA
                     GILAVQFGWRAAFAGVGIMTACYSLWVLLRQRAESRRGTRPVSRSVADDGTPIRRGFR
                     FVLLAGGLDNATRGAALTFLPFVLVDKGFDAAAISVLYGMIFAAGAAGKFACGWLSDR
                     RGILAVIVATESATAATLLALTWASRWAVIPLVLIFGFALNGTSSALNVAVAQFVPAA
                     KRARGFGIYFTAALISSAGAPLLYGVLADATSITTTFVAMALLTLAVLPAIGPIRGAL
                     VMGDGA"
     gene            2103..5216
                     /locus_tag="VFV93_00790"
     CDS             2103..5216
                     /locus_tag="VFV93_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5163901.1"
                     /translation="MRQALPVIGDFLQADTQILRWRSDRLFTLDILDFERAIAQAGAA
                     EREGDPRALRSALVQAIQTYGGDLLPGCYDEWLLDERERLRLQYDTALERLVDCLEEQ
                     REYALAITYAQRLLRRDPLHEATYRRLMRLHGLNNDRASALRVYQQCVTTLRHELAVE
                     PSPATLDAYERLSRIDDESSPASRNRTATGRPHLVGREREWRKLQAVWDTASRGNARL
                     VLIAGEAGIGKTRLAEELLAWAEAQGLATARARSYAAEGRLAYAPIADWLRSAAIRPG
                     VAHLDAVWRTAIARLLPELLAEDPCLERPPPLTESWQLQHFYAALAQAVATAAQPLLL
                     LIDDLQWSDPGTLEWLHYLLRFDREARLLVAGTVRDEEVGPDHPLHALALELRREELL
                     ADLPLGSLDVAATATLAAGIAGRELDAAAATRLYRETEGHPLFIVETVRAGGEAGAMP
                     GTPAVPDLAPLDTPEDERGSLPPKVQAVIAARLARLSPPAREIVGLAATVGRAFTAAV
                     LNEASVDDDEYVTGALDELWQRQILREQGADAYDFSHDKIREVAYAEINPARRRLLHR
                     RVARALELLYADDLDPVSGQLAAHYERAGELEQAVAYYHRAAEILQEVYANVDAIRLL
                     ERGLTLLARLPGKATRDVQELALLTTYSVSLNALKTFDAPTGDERYGAEMDHVYARVT
                     ELSERLGITVDPRTLRTMALVLITRGQVRRSLALGEQLLDIARHSAADPVLRTEAHYT
                     IGAASHWLGQFRAAREHLDAALTYYDRQRHRIHIVHYAQDPGAICLIRLAYTLLHLGY
                     PEQAKTRADEALALARELQHPQSLAYTLNWFSLIALELRDLQTARERINEAMDVAREH
                     QLGTWQLIGNPTLGLTLALAGDVHGAVDRIRQGISGDYATGQLLHAPQALAQLTDAYR
                     LLGAAGEGLDAVAEAFRLVERTDVRFIEASLHRLKGELLLLQGGNERAAETCFRRALA
                     VARQQEARMLELRAALSLGRLLQQQGGEGEARETLDAIYGWFSEGFDTPDLRDARELL
                     TTLQTLN"
     gene            5294..6715
                     /locus_tag="VFV93_00795"
     CDS             5294..6715
                     /locus_tag="VFV93_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5163902.1"
                     /translation="MTIPRTSRDTTRQLDDSRVKLLRERLRGALLQPEDPGYDAARAV
                     YNAGVERYPALIVQCMTVADIAAGVNFAREHELLLAIRGGGHSVSGLGTCDGGIVLDL
                     SRMHGVDVDPREKTARIEGGATWSQVDHATHAYGLATPGGVVSTTGVAGLTLGGGFGH
                     LSRHYGLSCDNLLSADIITADGQLRTVSDWQEPDLFWAIRGGGGNFGVVTSFTFQLHP
                     VDMIVGGPLFYSPEQAADVLRGYREYMRQAPDEMSAFFGFHLIPPAPFIPAEMHFKPS
                     AVIVVCYSGRDLQRGEHIVEQLRGLGTVIVEHVGPMPYPALNSAFDAIVPPGLHHYWK
                     ADYVSTLTDAAIDVHATYGPKVPSVQSAMHLYPQTGAIQRIGKSDSAYWHRDVEYIHN
                     IVAADTDPGRTGEHMVWMQGYYDALRSLSEAGGYVNFMTDDEGQERIKATYGANYDRL
                     LRVKKIYDPDNLFRVNQNIWPSS"
     gene            6843..8219
                     /locus_tag="VFV93_00800"
     CDS             6843..8219
                     /locus_tag="VFV93_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5163903.1"
                     /translation="MDDREQTVTGRTSLGASRDVLQHRLTRRGLLRGAVGLGGGALAL
                     SATPGVLAGAGYAAPLRTVNQYGPEVALEWFQLMLDIVLTTPRFSPAVANRIFAYLGV
                     TLYEAIVPGIPDGKSLAGRLNELTRVPGPRDHAYHWPTCANSALAQGMRDMFRTTTDA
                     NKAAINDLESSIAADLSHGVPFGITKRSVRRGRTVADHIMAWAATDAANSPTEAYVPP
                     VGPGLWVPTPPAFAGPVDPYGGTLRPIAVGPIENYDSGPPPAFSTDPQSEFYAGEMEV
                     YNTVNNLTDEQRTIALYWSRTAGHNISIAMRAVEQSGCNLGTAALALASSAIGSHDGG
                     IAVFYSKYTYNLLRPITYIREYIDPSWGDPLPLTTPPHPDFPAAHATVMRAFAQGLYD
                     VLGNFAFTDRTWVNVGLAPRSWNSWFEMAEETAISRLYGGIHSRWAIEAGLEQGRRVG
                     EAVTAVLD"
     gene            complement(8415..>9135)
                     /locus_tag="VFV93_00805"
     CDS             complement(8415..>9135)
                     /locus_tag="VFV93_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5163904.1"
                     /translation="NEATLQRYGLRAIPFLDSIGLLDTDLIAVHLSTTTPEEVELVAK
                     RGARMICCSNSIGIIDGVVPPAWGFKQAGGLVGLGSDQAPGNNSHNVFSEMRATAIFA
                     KIKAESPLPLPAWQVLRMATIEGATVLGIGDRVGSLEVGKEADLIMLDLTRPPLAPVL
                     LRPARNLVPNLVYAETGSNVVLSMVAGNVIYRDGEYTNIDRQAVVADLKEASRLLEEA
                     AAADPFVQDLPIVQLTREGRI"
BASE COUNT         1727 a         3017 c         2864 g         1527 t
ORIGIN      
        1 atcgtcgtca cggcgatgat cccgtggccg cacatcgtcg agtagccctc gttgtgcatg
       61 aagaggatcc cgtagtccgc ctcggctgtc accggcggcg tcaggatcgc gccgtacata
      121 tcggcgtgcc cgcgcggctc ccacatcagc gcccggcgga tgtcgtcgtg ctgctccagc
      181 agctcgcgtc gcaggtcgag gatcgttgca ccccgcaacg gcggcacgcc ggaggtaatc
      241 agccgcaacg gctctccggc ggcgtgtgcg tcaatcgtcg tcaacgtccg tcgtggttgg
      301 ctcacggatc acctcaatcc cgggcatagg ggatgagcgg aggctagccg catcccccat
      361 ccctcatgcc ccatccccca tcaccagcgc cccgcgaatc ggcccgatcg caggcaggac
      421 ggccagcgtc agcagggcca tcgccacgaa cgtggtcgtg atgctggtcg cgtcggccag
      481 gacgccatac agcagcggcg caccagccga gctgatcaac gccgccgtga agtagatgcc
      541 aaagccccgt gcccgcttcg cggccggcac gaactgagcg acggccacgt tcagcgccga
      601 cgaggttcca ttcaacgcga aaccgaagat cagcaccagc gggatgactg cccagcgcga
      661 cgcccacgtc agcgccagca gcgttgccgc cgtcgccgac tcggtcgcga caatcacggc
      721 cagaatgccg cgccggtcgc tgagccagcc gcaggcaaac ttcccggccg cgccggccgc
      781 gaagatcatg ccgtacagca cgctgatggc cgccgcgtcg aaccccttgt caacgaggac
      841 gaacggcagg aacgtcagcg ccgcgccacg cgtcgcgttg tccagcccgc cggccagcag
      901 cacgaagcgg aacccgcgcc ggatcggagt accgtcgtca gcgacactcc ggctcaccgg
      961 cctcgtcccc cgtcgcgact cggcccgctg gcgcagcagc acccagagtg agtagcaggc
     1021 cgtcatgatc ccgacgccgg cgaaggctgc ccgccagccg aactggaccg ccaggatacc
     1081 ggccacgaat ggtccgatca gcttgccgag atccccggcg aagttcagcg tccccatcga
     1141 ggtcgccaac tgctcgcgcc ggacgtggtt ggagacgatc gaggccgcca gcgggtgctg
     1201 ggcattgccg ccaatgccgg tcaggaaggc caccgccagc aacaggacaa acgtgccggt
     1261 cagcgccatc ccgaccaggc ccaggcccac ccagaggttg cccagcagca gaatcagcat
     1321 ctcaccgtag cgcccgccgg cgatcccggc cggcacctgc agcacgcttg acgctccgga
     1381 gaaggtcgcc ttcagcaacc cggtctgcgt gtacgagagg ttgagatcct cggcaatgaa
     1441 gggcagcagc gggtagagca gcgcgaagca ggcatcgttc gtcaggtgaa cgacagaggt
     1501 agttaccaga accgacgaat ccgatagcgc cccggagacc cgggcacgca cgcgcagcat
     1561 cttcaaccgt ctccgatcag atcacatccc ctatcgcgcc gctacgctac cacacgaaca
     1621 tcatctgcac gcaccgctga tcccgcgcgt tcccgccata ccgtcgccga ccgccaatct
     1681 gctcgggcgc gatgtgctgg ccctgatgcc ggaacggcaa ccggccgaag cgtgagcacg
     1741 acttgatttc atctctggtg actacgtatg tgcagagcag attagacgca attgcggtta
     1801 caataaacgc gtagccacag gattttacga ccaacgaata gggagattca gcacagcata
     1861 gcggctgctg tgaggaggct gacctgctac cgacactgca catccaactg ctgggagggt
     1921 tccagattcg gcgcggcgat gaaccgattg ccgccatcaa cgcaggacgc cagcaatcac
     1981 tgctcgccta tctgatcctg caccgtgaca ccgtcctctc tcgtcggcag attgccttca
     2041 gcttctggcc ggagtcgacg gaggcgcagg cacgcactaa tctgcgcaat gttgtgcatc
     2101 agttgcgtca agcgctgccg gtgatcggtg acttcctcca ggcggacacg cagatactgc
     2161 gctggaggtc cgatcgattg tttacgctcg acatcctcga cttcgagcgt gccattgctc
     2221 aggcgggagc ggccgagcgc gaaggagatc cacgcgcgtt gcgttccgca ctggtgcagg
     2281 caatccagac atatggcgga gacctattgc ctggctgcta cgatgagtgg ctgctcgacg
     2341 agcgcgaacg actgcgcttg cagtacgaca cggcgctcga acggctggtc gactgtctcg
     2401 aggagcagcg cgagtacgct ctggcgatca catatgccca gcgcctgcta cgccgtgatc
     2461 cgctgcatga ggcgacctac cgccgcctga tgcggttgca tgggctgaac aacgaccgcg
     2521 ccagcgcgct gcgcgtctac cagcagtgcg taacaacctt acgtcacgaa ctggccgtcg
     2581 aaccgagccc tgccaccctc gatgcctacg agcgactatc acggatcgac gacgagtcgt
     2641 ccccagcatc ccgcaacaga acggcgactg gcagaccaca tctggtcgga cgtgagcgag
     2701 agtggcgcaa gctccaggcg gtttgggaca cggcctcgcg cggaaacgca cgcctcgttc
     2761 tgatcgctgg cgaagctggc attggcaaga cgcggctggc cgaggagttg ctcgcctggg
     2821 ctgaggcgca agggctggcc acggcgcggg cacggtcata tgctgcggag ggacgcctgg
     2881 cctacgctcc gattgccgac tggctgcgga gtgcagcgat tcggcctggc gtcgcgcatc
     2941 tggatgcggt ctggcgcacc gcgatcgcgc ggctgctgcc ggaactgctt gcagaggacc
     3001 cctgtctgga gcgcccgcca ccgctgaccg agagctggca gctacagcac ttctacgctg
     3061 ccctggccca ggcggtcgct acggctgccc agccactgct gctgctgatc gacgatctcc
     3121 aatggtctga tccaggcacg ctcgaatggt tgcactacct gctgcgcttt gatcgtgaag
     3181 cacgactgct ggtcgccggc acggtccgcg acgaggaggt cggtcccgat catccgctgc
     3241 acgcactggc gctcgagcta cgccgagaag aattgctggc cgatctgcca ctggggtcac
     3301 tggatgtcgc ggcaaccgcc actctggcag ccgggatcgc cggacgggaa ctcgacgccg
     3361 cggcggcgac gcgattgtat cgggagacag aaggtcaccc actcttcatt gtcgagacag
     3421 tccgtgccgg cggcgaagcg ggcgcgatgc caggcacacc agccgttccg gatctcgcac
     3481 ccctcgacac tccggaggat gaacgcggtt cgctgccgcc gaaagtccag gccgtcatcg
     3541 ctgcccggct ggcgcgattg tcgccgcccg cgcgcgagat cgtcggactg gctgcgaccg
     3601 tcgggcgtgc gtttacggcc gccgtgctga acgaggcaag cgtggatgac gacgagtatg
     3661 taacgggcgc gctcgacgaa ctctggcagc gtcagatact gcgcgagcag ggcgcggacg
     3721 cctacgactt cagtcacgac aagatccgcg aagtggccta cgccgagatc aacccagcgc
     3781 gacgccgcct gctgcatcga cgcgtagcgc gtgccctgga gctcctgtat gccgacgatc
     3841 tggatcccgt cagcggacag ctggcggcgc attacgagcg tgccggagaa ctggagcagg
     3901 cggtagcgta ctatcatcgt gctgctgaga tcctgcagga agtgtatgcc aacgtggacg
     3961 cgatccgctt gctcgagcgc ggattgacgc tgctggcgcg tttacccggg aaagccacgc
     4021 gcgacgtgca ggaacttgct ctgctgacca cctacagcgt ttccctgaac gcgctgaaga
     4081 cgttcgacgc gcccacaggt gatgagcgct atggcgccga gatggaccac gtctacgccc
     4141 gggtaaccga gctcagcgag cggcttggca ttacggtcga tccccgaacc ctgcggacga
     4201 tggcactcgt cctcatcacg cgcggacagg ttcggcggtc tctcgcgttg ggtgagcaac
     4261 ttctggatat cgcccggcat agcgccgcag acccggttct gcgtaccgaa gcgcactata
     4321 cgatcggtgc cgcgtcccac tggctcggcc agttccgagc tgcccgggag cacctggacg
     4381 cggcgttgac gtactacgat cggcaacgcc atcgtatcca cattgtccac tacgcccagg
     4441 atccaggcgc gatctgcctc atccgcctgg cgtacacgtt gctgcacctc ggttaccccg
     4501 agcaagccaa aacgcgcgcc gatgaggcgc tggcgctcgc ccgcgaactg cagcatccgc
     4561 agagtctggc atacaccctg aactggttct cattgatcgc gctggaattg cgggatctgc
     4621 aaacggctcg ggaacggatc aacgaagcga tggacgtggc cagggagcat caacttggaa
     4681 cctggcaatt gatcgggaat cccaccctgg gcctgacgct ggcgctggcc ggagacgtcc
     4741 acggagcagt tgaccggatc cgccagggca tttccggaga ttacgcaact gggcagttgt
     4801 tacacgcgcc tcaggcactg gcacaattga ccgatgccta tcgtttgctg ggagcggctg
     4861 gcgagggact tgacgcggta gccgaagcgt tcaggctcgt ggaacgcacc gacgtgcgct
     4921 tcatcgaggc cagtctgcac cggctgaagg gcgaactttt gctccttcag ggcggcaacg
     4981 agcgtgctgc cgagacctgt ttccgccggg cactggccgt cgcacgccag caggaggctc
     5041 ggatgctgga actgcgtgca gccttgagtc ttggccggct attgcagcag cagggcgggg
     5101 aaggcgaagc gcgcgaaacg cttgacgcga tctacggctg gttcagcgag ggcttcgata
     5161 caccggatct gcgcgacgcc cgggagttgc tgacaacgct tcaaacgctc aactagacgc
     5221 ctttcaaacg catcttcaca taggctgagg cccaggccat caggcaaatg gaagatgcag
     5281 aaaggaggaa ggcgtgacaa tcccgcgtac cagccgggac acgacccgac agcttgatga
     5341 tagccgcgtc aagctgttac gcgagcgatt gcgcggcgcg ttgctgcagc cagaggaccc
     5401 tgggtatgat gctgcccgtg ccgtctacaa cgccggtgtt gagcgctatc cggcgttgat
     5461 cgtgcagtgc atgacggttg ccgatatcgc cgctggggtg aacttcgccc gcgaacatga
     5521 gttgcttctg gccatccgtg ggggcggcca tagcgtctcg ggcctcggca cctgcgacgg
     5581 cggcatcgtc ctcgatctga gccggatgca cggtgttgac gtcgatcccc gcgagaagac
     5641 cgcccgcatc gaaggtggcg caacctggag ccaggtcgat cacgcgacgc acgcatacgg
     5701 tctggcgacg ccgggcggcg tcgtttcgac gaccggggtc gccgggctga cgctcggcgg
     5761 cggcttcggt cacctctcgc ggcactacgg cctgtcgtgc gacaacctgc tctcggccga
     5821 catcatcacc gccgacgggc agctgcgcac cgtcagcgac tggcaggaac cagacttgtt
     5881 ctgggcgatc cgtggaggcg gcggtaactt cggcgtcgtc acctccttca cgtttcagct
     5941 ccacccggtc gatatgatcg tcggcggccc gctgttctac tcgcccgagc aggctgccga
     6001 tgtgctgcgc ggctaccgcg agtatatgcg gcaggcgccg gatgagatga gcgcgttctt
     6061 cggcttccac ctcatcccgc cggcgccatt catcccggca gagatgcact tcaagcccag
     6121 cgccgtcatc gtggtctgct attcgggtcg tgacctgcag cggggtgagc acatcgtcga
     6181 gcagcttcgg ggtctcggga cagtgatcgt tgaacacgtc ggcccgatgc cctatccagc
     6241 gctcaacagc gcattcgacg cgatcgtgcc accggggttg catcactact ggaaggccga
     6301 ctatgtctcc acgttgactg atgcagccat cgacgtgcac gcaacctacg gcccgaaggt
     6361 gccatcggtg cagtctgcga tgcacctcta tccacagacc ggcgcgatcc agcggatcgg
     6421 caagagcgat tccgcctact ggcaccgcga tgtcgagtac atccacaaca tcgtggcggc
     6481 cgacactgat ccaggccgga ccggcgagca catggtctgg atgcagggct actacgacgc
     6541 gctgcgctcg ttgtcagaag cgggcggcta cgtcaacttc atgactgacg atgaaggtca
     6601 ggagcggatc aaggcaacct acggagccaa ctacgaccga ctcctgaggg tcaagaagat
     6661 ctacgaccca gacaacctgt tccgcgtcaa tcagaacatc tggccaagca gctagcgatc
     6721 tgactcgcgt cggaaacagg acacctgact gccgatctat caaccgtcgt ggctcaggcc
     6781 cgtcggaaga ccttacgccc ccaaccgaac gttcgagcca tcaccgtgaa aaggaaagcg
     6841 acatggacga cagagaacag acagtcaccg gacggacgag cctcggcgct tcgcgcgatg
     6901 tgctgcagca ccgactgaca cgacgcggcc tgctacgagg agcggtcggg cttgggggcg
     6961 gagcgctagc gctcagtgcc acgccaggcg tcctggccgg ggccgggtac gcagctccac
     7021 tgagaaccgt caatcaatac ggcccagaag tcgccctcga atggttccag ctcatgctgg
     7081 acatcgtgct gacgacacct cgcttctctc cggccgtggc aaaccgaatc tttgcgtatc
     7141 ttggggtaac gctgtacgag gcgatcgtac ccggtatccc ggatggtaag agcctcgctg
     7201 ggcgtctcaa tgaactaacg cgggtacccg gtccccgcga ccatgcgtat cactggccaa
     7261 catgcgccaa cagcgcactc gctcagggca tgcgcgacat gttccgcacg acgaccgatg
     7321 ccaacaaggc tgcgatcaat gacctggagt cctcgatcgc agcggacctg tcgcacgggg
     7381 tgcccttcgg aatcaccaag cgatcggtgc gtcgaggccg cacggttgcc gatcacatca
     7441 tggcctgggc ggcaaccgac gccgcgaaca gcccgacgga ggcgtacgtg ccgccggtcg
     7501 gtccgggcct ctgggttcct actcccccag ccttcgcagg tcctgtcgac ccgtacggag
     7561 gcacactgag accgatcgcg gtgggtccga tcgagaacta cgattccggt ccgccaccag
     7621 cgttctcgac cgatccgcaa tcggagttct atgccgggga gatggaggtc tacaacacag
     7681 tcaataatct caccgacgaa cagcggacca tcgctcttta ctggtcccgg acggcgggcc
     7741 acaacatctc gattgcgatg cgggccgtcg agcagtcggg gtgtaacctc ggcactgcgg
     7801 cactggcact ggcgagctcg gcgatcggtt cccacgatgg tggtatcgcc gtcttctact
     7861 cgaagtacac ctacaatctg ctccggccga tcacctacat ccgggagtac atcgatccga
     7921 gctggggcga cccactgccg ctgacgacac caccgcatcc ggatttcccc gccgcgcatg
     7981 caacggtgat gcgagcgttc gcccagggac tctatgacgt tctcggtaac ttcgccttca
     8041 ccgaccgcac ctgggtcaat gttggcctgg ctccacgctc gtggaactcc tggttcgaga
     8101 tggccgaaga aaccgcgata tcccggttgt acggaggtat ccacagccgc tgggcgatcg
     8161 aagcgggact cgaacaaggg cgacgggttg gcgaagccgt gaccgccgtc ctcgattgag
     8221 cacacctccg gcaactcaac cagcagatga gtcggaactg acatcgccga gcctgaacga
     8281 tccggccgag aacccgacgc aaaggtgggc ggcagcgcga tgtgtcgctg ccgcccacct
     8341 atctctaacc tcgaaaccag ccttgagacc tcagctgtag ctgcatgacc aggtattcgg
     8401 caaggcacac gcggctagat ccgtccttca cgtgtcaact gaacaatcgg gagatcctgg
     8461 acgaacgggt cggcggcagc ggcctcttcg aggaggcggg aggcttcctt caggtctgca
     8521 acgacggcct ggcggtcgat gttggtgtac tcgccatcgc ggtagatcac gttgccggcc
     8581 accatgctga ggacgacgtt gctgcctgtc tcggcgtaga cgaggttcgg gaccaggttg
     8641 cgcgcgggcc ggagcagcac cggtgccagc ggcggccggg tcaggtcgag catgatcagg
     8701 tcggcctcct tgccgacctc caggctgccg acgcggtcgc cgatcccgag gacagtcgcc
     8761 ccttcgatcg tcgccatccg cagcacctgc caggccggca gcggcagcgg gctctcggcc
     8821 ttgatcttgg caaatatcgc cgtcgcgcgc atctcgctga agacgttgtg tgagttgttg
     8881 cccggtgcct ggtcggagcc gagccccacc aatccaccgg cctgcttgaa tccccaggcc
     8941 ggcgggacaa cgccatcgat gatcccgatc gagttcgagc agcagatcat ccgcgcgccg
     9001 cgcttcgcga ccagctctac ctcctccggt gtcgtcgtcg agaggtgaac ggcgatcaga
     9061 tccgtgtcga gcaggccgat cgagtcgagg aacggaatcg cccgcagccc atagcgctgg
     9121 agcgtcgcct cattc
//
LOCUS       DASUGJ010000029         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_281104, whole genome shotgun sequence.
ACCESSION   DASUGJ010000029 DASUGJ010000000
VERSION     DASUGJ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281104"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1437
                     /locus_tag="VFV93_00810"
     CDS             133..1437
                     /locus_tag="VFV93_00810"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX5163905.1"
                     /translation="MQETQTRIDVDKESQGSHLLRDAIAHVRAAAKRLGLNDGLLQVL
                     ETPEREINVALPVEMDNGTIRVFHGYRVQHSRLLGPAKGGFRYHPDVDIDEVRGLASL
                     MTWKCSLLNLPYGGAKGGVSVDPTVLSTSELARLTRAYAAALAPVIGSRVDVPAPDVN
                     TNDKTMGWFLDEYERVSGNYDPAVVTGKPIALGGSEGRGESTGRGVALAAKLMLTRVG
                     IPLDEARIVVQGYGKVGFDTVRFLADAGCRIIAISDVSGGLYNPEGLDIPRINEHVNR
                     HPKRLLEGYVGDDAETISNADILTLECDALIPAALEGQITGANADHIRARIIVEGANG
                     PTTSEADVILAKRGILVVPDILANAGGVVVSYFEWLQGLQGQRWTLADVREKLDAQMT
                     EAFNSVVVRADQEHITLRQAAFLIALERVATAARLRWHVAAA"
     gene            1493..2050
                     /locus_tag="VFV93_00815"
     CDS             1493..2050
                     /locus_tag="VFV93_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163906.1"
                     /translation="MSFTPRSTVDLAEVTPQMVNAAVAVATGRLRLGNVTETRRRLIA
                     GDADASMYFRFELARQVASLLLSLDSNVIAVYEEQDVPSAEECAPEQPTMFEPISLIV
                     RVELRTSALDAIVNALNDALSDAIGRDLTLTPRGYLDIQVVTERDARQLRPRAFGYRP
                     APLLLAAREERADSDDLANLQRLWR"
BASE COUNT          415 a          777 c          754 g          405 t
ORIGIN      
        1 agtctctcat gacattctca tacctccccc atccgctctc atccggctct cacggccggt
       61 aaggcaggat tgatggtgca aaccgaaacg tgcgccgttg cacgtcacga tagaggatga
      121 gtgagcgtac agatgcagga aacacagacg aggatcgacg ttgacaagga atcgcagggt
      181 tcgcacctgc tgcgtgatgc gatcgcgcat gtgcgggcgg cggcgaagcg gctgggcctg
      241 aatgatggcc tgctgcaggt ccttgagacg ccggagcggg agatcaacgt cgcgctgccg
      301 gtcgagatgg acaacgggac gatccgcgtc ttccacggct atcgcgtaca gcattcgcgc
      361 ctgctggggc cggccaaggg cggcttccgc taccacccgg acgtcgatat cgacgaggtg
      421 cgtggcctcg ccagcctgat gacctggaag tgctcgctgc tcaacctgcc gtacggcggc
      481 gccaagggtg gcgtctcggt cgatccaact gtgctgtcca ccagcgagct cgcccggctg
      541 acgcgtgcct acgccgctgc tctcgcgccc gtcatcggct cgcgggtcga tgtcccggcg
      601 ccggacgtca acacgaatga caagacgatg ggctggttcc tcgacgagta tgagcgcgtc
      661 agcggcaact acgacccggc tgtcgtgaca ggcaagccga tcgcactggg tggcagcgag
      721 ggacgtggcg agtcgaccgg ccgtggcgtc gcgctggcgg cgaagctgat gctcacccgg
      781 gtcggtatcc cgctggacga ggcgcgaatc gttgtccagg gctacggcaa ggtcggattc
      841 gacactgtcc gcttcctggc cgatgctggt tgccggatca tcgccatcag cgatgtcagc
      901 ggtggactct acaaccccga aggtctggac attccacgca tcaacgagca tgtgaaccgg
      961 catccgaagc ggctgctgga aggttacgtc ggcgacgatg ccgagacgat ctcgaacgcc
     1021 gacatcctga cgcttgagtg cgacgcgctg atcccggccg cgctcgaagg acagatcacc
     1081 ggcgcgaacg ccgatcacat ccgggcacgc atcatcgtcg agggcgccaa cggcccgacg
     1141 acgagcgagg cggacgtcat tctcgccaag cggggcatcc tggtcgtgcc ggacattctc
     1201 gctaacgccg gtggcgtcgt cgtctcctac ttcgagtggc tgcagggcct gcaggggcag
     1261 cgctggacgc tggccgatgt gcgcgagaag ctcgatgccc agatgaccga agccttcaac
     1321 agcgtcgttg tccgtgccga ccaggagcac atcacgctcc gccaggcagc gttcctgatc
     1381 gcgctcgaac gggtcgcgac ggccgcccgc ctgcgctggc acgttgccgc agcctgatgt
     1441 ctcgatggcc tacggcctgt tgcctgtggc ctcgaaagag gatccaatga gcatgtcatt
     1501 cacaccacgc tccacggtcg atctggccga ggtgacacca cagatggtca atgcggctgt
     1561 tgccgtcgcg accggccggc tgcggctcgg caacgtcacc gagacgcgcc gtcgcctgat
     1621 cgccggtgac gccgacgcca gcatgtactt ccgcttcgag ctggcccgcc aggtcgcctc
     1681 gctgctgctc tcgctggaca gcaacgtcat cgccgtttac gaggagcagg acgtcccatc
     1741 ggccgaggag tgcgcgccag agcagccgac gatgttcgag ccgatcagcc tgatcgtgcg
     1801 ggtcgagctg cgtacctccg cgctcgacgc gatcgtcaac gcactcaacg acgccctcag
     1861 cgacgcgatc ggccgcgacc tgacgctgac gccacgcggc taccttgata tccaggtcgt
     1921 caccgagcgt gacgcccgcc aactccgccc gcgcgccttc ggataccgcc ccgcgccgtt
     1981 gctcctcgcc gctcgcgagg aacgcgcgga ctccgacgac ctcgcgaacc tccagcgcct
     2041 ctggcgctga tcctcacacg attcctacta ccgccggggg gaggaacgaa gggccgtgtc
     2101 attatgacgc ggcccttcgt ttgtgttggg gtcgtgacgc gtgatgcgtg acgcgaagtt
     2161 cggatcgatg gccttaagac ccgtcacgcg tcacgacccc cgagaccgaa tcactggtca
     2221 gaaatactca tttccacaac cgccgtgtga ttggttgttc acacgcgcaa tgcgcactgg
     2281 taggatgaca gtattcggtg catcaactcg cggagttgga ccgccgccgc ccagccaggc
     2341 ggggcgtgtt t
//
LOCUS       DASUGJ010000030         5988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1809630, whole genome shotgun sequence.
ACCESSION   DASUGJ010000030 DASUGJ010000000
VERSION     DASUGJ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809630"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VFV93_00820"
     CDS             complement(<1..983)
                     /locus_tag="VFV93_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX5163907.1"
                     /translation="MPSLQIVSDLKPTGDQPAAIDQLVAGLEQHQRHQTLLGVTGSGK
                     TFTMANVIERVQRPTLVLAHNKTLAAQLYSEFKEFFPNNAVEYFVSYYDYYQPEAYIA
                     RTDTYIEKDAQINDEIDKLRHAATRALLSREDVIIVASVSCIYGLGSPSDYEEQVVKL
                     KRGQAVRRDRVLRHLIDIQYDRNDMTLVRGTFRVRGDTVEVYPVYEEIAVRIEFFGDE
                     VERIVELDPLTGEILVERTEIDIYPAKHFVTTDEKLKVAIVDIEAELEEQLEFLESRG
                     KVLEAARLKQRTRYDIEMLQQAGYCSGVENYSMHLGRRKPGQTPWTLLDYFP"
     gene            complement(1085..1549)
                     /locus_tag="VFV93_00825"
     CDS             complement(1085..1549)
                     /locus_tag="VFV93_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5163908.1"
                     /translation="MPRIVSDLVDTYLFRKINARPQFLVLRRRSDTPNGDSWQSIHTR
                     VAGGETAIDAARRDVQASTGLVPVRFYTADYIAQFYDHASDSIIFAPTLAAQINPKSS
                     ISIAPEYSDYAWCDLEETTSRLIYSAQRWAVRHIYDVIAMGGEESDIYAIES"
     gene            complement(1632..2111)
                     /locus_tag="VFV93_00830"
     CDS             complement(1632..2111)
                     /locus_tag="VFV93_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163909.1"
                     /translation="MPEKRYTKVEEEVIQILDRLESEKPSGSRPNLRLVESPRSRKQP
                     SRWKSIKPSFNLSRVVAGRPWIWIAASIGLAFVALLVRDTSENLALALALASIVAFFA
                     PIFVNRRGSGSYGSSSSPGEVKQWRGRDISFDPPDGPSPAERLSRWFQDRRRGGPRL"
     gene            2440..2901
                     /locus_tag="VFV93_00835"
     CDS             2440..2901
                     /locus_tag="VFV93_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163910.1"
                     /translation="MTDGDTTTSFAGPGHAIKMLVAACAKGPRTVRELLEQTRPYDEH
                     FATDVLNGLAVFDEHNVRDNTDSIERKLQHQQPDEWPPFRVYNDVTRRACTQPGRAGL
                     IVINLAAKRIIQVQNSYAEIQRQDRGRIRAGGKPTRVLYHYDLPKDWALVP"
     gene            complement(2950..3993)
                     /locus_tag="VFV93_00840"
     CDS             complement(2950..3993)
                     /locus_tag="VFV93_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX5163911.1"
                     /translation="MQKVTIVGLGLIGSSIGLGLRRWATNEGKRSAVLEVTGFDLDLD
                     QQNYSKKLKAVDRAEWDLTRAVRDADLIVLAVPPLAVHDVFESIAPHLKDNVVITDTT
                     STKVQPLEWARELLPKTAHFIGGHPMAGKSQSVEGAEADLFNGATWCIVPSVEADESA
                     VQTVLGMVSALGAEPMFVDAHEHDSMVAGISHLPFVLSIALMRSVSGDPGWRDMKFLT
                     AGGFRDASRLAAGSPEMHRDITATNREAVTRWIDTCVDELQHIRSLIHANTSEADETL
                     LARFREARDARADWATAERREGQLVQDTESELSKASVSEQMSQMFVGGMFRRRPRMNG
                     SSRDRDRPGRDRD"
     gene            complement(4072..4230)
                     /locus_tag="VFV93_00845"
     CDS             complement(4072..4230)
                     /locus_tag="VFV93_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163912.1"
                     /translation="MAISIVICLAAFIWIFVEIEPYMRDFTGSDVVVTPGPDDPFATP
                     VEPIDQPD"
     gene            4493..>5988
                     /locus_tag="VFV93_00850"
     CDS             4493..>5988
                     /locus_tag="VFV93_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5163913.1"
                     /translation="MAQFKNFINGKWVDAKSGQEFDRKNPATGETVGTFAKSEQADVV
                     AAIEAASAAFNRWRLYPAPKRGEILFKVGQLLIERKEQLAREMTEEMGKVIAEARGDV
                     QEGIDMAFYMAGEGRRQWGQTVPAEMPNKWAMSVRKPLGVVAAITPWNFPLAIPTWKI
                     MPALVTGNTVVFKPASYTPRMAVRLVEIFEEAGLPAGVLNLVLGSGPDTGDTLLQHPD
                     VALVSFTGSTETGTHISVEGARQLKRVSLELGGKNAIIVMDDADVDLAVEGILWSAFG
                     TSGQRCTAASRVIAHKGLSDELTGKLAERAGAMRLGYGLEDSTDVGPVVSRSQLERVH
                     SYIAVGQGEGADLAIGGQIAGDGDLGRGHFHQPTVFGNVKPDMRIAQEEIFGPVTAVI
                     PVSDVEEAINVANGVQYGLSASIYTRDVNRAFRAMQDIYTGILYINAGTIGAEIQLPF
                     GGTKATGNGHREAGQAALDVFTEWQSIYVDYSGKLQRAQIDEVEDNGMA"
BASE COUNT         1078 a         1822 c         2020 g         1068 t
ORIGIN      
        1 gggaagtagt cgagcagcgt ccacggcgtc tggcctggct tgcgacgacc gagatgcatc
       61 gagtagttct cgactccgga gcagtagccg gcctgttgca gcatctcgat gtcgtagcgc
      121 gtacgctgct tcaaccgtgc cgcttcgagg accttcccac gcgactccag gaactcgagc
      181 tgctcttcga gctcggcttc gatatcgacg atcgcgacct tcagcttctc gtcggtcgtg
      241 acgaagtgct tggcggggta gatgtcgatc tcggtccgct cgaccaggat ctcgccggtc
      301 agcgggtcga gctcgacgat ccgctcgacc tcgtcgccga agaactcgat ccgaaccgcg
      361 atctcctcat agactggata gacctcgacc gtgtcgccac ggacacggaa cgtcccgcga
      421 accagcgtca tatcgttgcg gtcgtactgg atgtcgatca ggtggcgcag gacgcgatcg
      481 cggcggacgg cctgcccacg tttgagcttg acgacctgct cctcgtagtc ggacggcgag
      541 ccgagaccgt agatgcagga gacggaggcg acgatgatga catcctcgcg agagagcaac
      601 gcgcgggttg ccgcgtggcg cagcttgtcg atctcgtcgt tgatctgggc gtctttctcg
      661 atgtaggtat cggtgcgggc gatgtacgcc tcgggctgat agtagtcata atacgagacg
      721 aaatactcga cggcgttgtt agggaagaac tccttgaact cactgtagag ctgggctgcc
      781 agcgtcttgt tgtgggcgag gacgagcgtc gggcgctgaa cgcgctcgat gacattcgcc
      841 atcgtgaacg tcttgccgga gccggtcaca cccagcagcg tctgatgccg ttgatgctgt
      901 tcgaggccgg cgacaagctg atcgatcgcg gccggctggt caccggttgg cttcaggtcg
      961 gagacgatct gaagggaagg catagcgtgg atctccgttc gtctggcgac aaacgagccg
     1021 gcctcgtgag ggggccggcg gtcgatccta agtataccgt tttccgcgtc cggacgcaga
     1081 gatttcagct ctcgatcgcg tagatgtccg actcctcgcc gcccatcgcg atgacatcgt
     1141 agatgtggcg gacggcccag cgttgggccg aatagatcag ccgcgaggtc gtctcttcga
     1201 gatcgcacca ggcgtagtcg ctgtactcgg gcgcgatgct gatcgatgat ttcgggttga
     1261 tctgggcagc cagcgtcggt gcgaagatga tcgaatccga cgcgtggtcg tagaactggg
     1321 cgatgtagtc ggcggtgtag aagcgaacgg gcacaagccc ggttgaagcc tgaacgtcgc
     1381 gccgggcggc gtcgatcgcc gtttcgccgc cggcgacgcg ggtgtgtatg gactgccagc
     1441 tgtcaccatt tggcgtgtct gaccggcgtc gcaggacgag gaactgcggt cgcgcgttga
     1501 tcttgcggaa cagatacgta tcgaccagat ccgacacgat cctcggcatg acggctacac
     1561 gatcctccca tgtccccggc cggcctcgcg accttcggag gaggatcgta gcacgccagc
     1621 gctgccgcct actagagccg cgggccgccc cgccggcgat cctggaacca gcgtgagagc
     1681 cgctcggccg gtgaaggtcc gtccggcgga tcgaaggaga tgtctcgccc gcgccactgt
     1741 ttgacctcgc caggtgagct actcgatccg tagctgcccg atccgcggcg attgacgaag
     1801 attggcgcga agaacgcgac gatgctggcc agcgccaacg ccagcgccag attctcagag
     1861 gtgtcccgga cgagtaacgc gacgaaggcg agaccgatgg acgctgcgat ccagatccac
     1921 ggccggccgg cgacgacgcg cgacaggttg aacgatggct tgatcgattt ccagcgtgac
     1981 ggctgcttgc gggagcgggg cgactcgacc aaccgcaggt tcgggcgaga tccggacggc
     2041 ttctcactct ccagccggtc gagaatttga atgacctctt cttcaacctt ggtgtagcgc
     2101 ttctcgggca ttctgggttc ctcccgctgc tgcctgtgct ggacgatgta cgagcgcacg
     2161 ggtggcgaca aacggtcaat cggcgcgtgc gtcaacgctc gaggtggcgg cagtgagcaa
     2221 atggcgcgcc gctggcgaag cctgtgccta gtgtacgaca cgcggggtgt gaatgggcct
     2281 cttgtcgaac gctgtcagcg acgccagaat cgccggcggc ctgtgataga gtaatcgact
     2341 tgccgggcgc tcagtccggc cagagtcgag ccagcgctgg cggctggcat gaggcaggat
     2401 ggacccgaga atcccgtggg tagcttgatt cgatttaccg tgactgatgg agatacgacg
     2461 acgagctttg ccggaccagg ccacgcgatc aagatgctgg tcgcagcctg cgcaaaggga
     2521 ccgcgaaccg ttcgagaatt gctcgaacag acccgtcctt acgacgagca ttttgcaacc
     2581 gatgtcctca acggactggc cgtctttgac gagcacaacg tccgcgacaa caccgactcg
     2641 atcgagcgca agctccagca ccagcagccc gacgagtggc cgccgttccg tgtctacaat
     2701 gacgtgactc gacgtgcctg cacccagccg gggcgagccg gattgatcgt catcaatctg
     2761 gcagccaagc gcatcatcca ggtccagaac agctatgctg agatccagcg ccaggatcgc
     2821 ggccggatcc gcgccggcgg caagccaacg cgtgtccttt accactacga tctgcccaag
     2881 gactgggcgc tggtgccgta acgcagcact gtcgtcctga ggctgcagac ccaggacgac
     2941 agtcctgcgc taatcccgat cgcggccggg ccggtcgcgg tcgcggctgg agccgttcat
     3001 acgcggacgg cggcggaaca tgccgccgac gaacatctgg ctcatctgct cgctgacgct
     3061 ggccttcgac aactcgcttt cggtgtcctg caccagctgg ccttcgcggc gctcggctgt
     3121 cgcccagtcg gcgcgggcat cacgggcctc gcggaagcgg gccagcagcg tctcgtcggc
     3181 ctcgctggtg ttcgcgtgga tcagagagcg gatgtgctgc aactcgtcga cgcaggtgtc
     3241 gatccagcga gtgaccgcct cacggttggt cgcggtgatg tcgcggtgca tctccgggct
     3301 gccggctgcc agccgtgagg catcgcggaa gccgccggcc gtcaggaact tcatgtcacg
     3361 ccagcccgga tcgccactga ccgagcgcat cagcgcgatt gaaagcacga acggcaggtg
     3421 gctgataccg gcgaccatgc tgtcgtgctc atgggcgtcg acgaacatcg gttcagcgcc
     3481 gagcgcgctg accatgccga ggacggtctg gacagccgac tcgtcggcct cgaccgacgg
     3541 gacgatgcac caggtcgcgc cattgaagag gtccgcctcg gcgccctcga cgctctgcga
     3601 cttgccggcc atcgggtgcc cgccgatgaa gtgggccgtc ttcggcagca gctccctggc
     3661 ccactccagc ggctgaacct tggtcgaggt ggtgtcggtg atgacgacgt tgtccttgag
     3721 gtgcggcgcg atcgattcga agacatcgtg aaccgccagc ggcgggacgg cgaggacgat
     3781 gaggtcggcg tcgcggacgg cacgggtgag gtcccactct gcgcgatcga cggccttcag
     3841 cttcttgctg tagttctgct ggtcgaggtc gagatcgaag ccggtgacct cgaggacggc
     3901 ggagcgcttg ccctcgttcg tggcccagcg gcgaagcccg agaccgatgg acgacccgat
     3961 caggccgagc ccgacgatcg ttaccttctg catgatgatc ctttcgaggc actggtggcg
     4021 tggttgcgtt gcgtcggtat cgctggtctc aaagtatagc agtggcgtcg attaatccgg
     4081 ctgatcgatg ggttcgaccg gtgtcgcgaa cggatcatcg gggccgggcg tcaccacgac
     4141 atcgctcccg gtgaagtcgc gcatatatgg ctcgatctcg acgaagatcc agatgaaagc
     4201 ggccaggcag atgacgatcg agattgccaa cgcaactgcg atcccgagat cactccgcag
     4261 aaaccggtgt gaagatggat catcgtcgac tggctctcgc atgaccgccc ccaggctgcg
     4321 ccggcgtggt tgaatcaggt agacgcgttg cgtcgcgttt cgcagtatag cagccacttc
     4381 cggggccgtt tgaggcgccc agatgctctg atacactgcg gcgcgagtcc agaacgagcg
     4441 tatgccggtc gccgtttgcc ggcagtagac gggtcggaga gggacggttt ccatggcgca
     4501 gttcaagaac ttcattaatg gcaagtgggt cgacgcgaag agcggtcagg agttcgatcg
     4561 caagaatccg gcgactggcg agacggtcgg gacgtttgcg aagagcgagc aggcagatgt
     4621 cgtcgcggcg atcgaggcgg cctcggcggc cttcaatcgc tggcggctct atccggctcc
     4681 gaagcggggc gagattctct tcaaggtcgg gcaactgctg atcgagcgta aggagcagct
     4741 cgcccgcgag atgaccgagg agatgggcaa ggtcatcgcc gaggcgcgcg gtgatgtcca
     4801 ggagggcatc gacatggcct tctacatggc cggcgagggc cgccgtcagt ggggccagac
     4861 ggtgccggcc gagatgccga acaagtgggc gatgtcggtc cgcaagccgc tcggcgtggt
     4921 ggccgcgatt acgccgtgga atttcccgct ggcgattccg acgtggaaga tcatgccggc
     4981 gctcgtgacc ggtaacacgg tcgtcttcaa gccagcgtcg tatacgcctc ggatggcggt
     5041 ccggctggtt gagatcttcg aggaggccgg gctaccggcc ggtgtcctca acctcgtgct
     5101 cggcagtgga ccggacacgg gcgatacgct gctgcagcac ccggacgttg cgctggtgtc
     5161 gttcaccgga tcgaccgaga ccgggacgca tatcagcgtc gaaggcgccc gccagctgaa
     5221 gcgggtttcg ctggagctgg gtggaaagaa cgcgatcatc gtcatggatg atgccgatgt
     5281 cgatctggcg gtcgaaggca tcctctggag cgcgttcggc acgtccggcc agcgctgcac
     5341 ggctgccagc cgcgtcatcg cccacaaggg cctctccgac gagctgaccg gcaagctggc
     5401 cgagcgggcc ggcgcaatgc ggctcggtta tgggctggag gacagcaccg atgtcgggcc
     5461 ggtcgtcagc cgctcgcagt tggagcgggt ccactcctac atcgccgtcg gtcagggcga
     5521 aggggccgac ctggcgatcg gcggccagat cgccggcgat ggcgacctgg gacgcggcca
     5581 cttccaccag ccgaccgtct tcggcaacgt caagccggat atgcgaatcg cccaggagga
     5641 gatcttcggg ccggtgacag ccgtgatccc cgtttcggat gtcgaggagg cgatcaacgt
     5701 cgccaacggc gtccagtacg ggctgtcggc gtcgatctac acccgcgacg tcaaccgcgc
     5761 cttccgggcg atgcaggaca tctacaccgg catcctctac atcaacgccg gcacgatcgg
     5821 tgcagagatc cagctcccgt tcggcggcac gaaggcaacc ggcaacggcc accgcgaagc
     5881 cggccaggct gcgctggatg tcttcaccga gtggcagtcg atctacgtcg actacagcgg
     5941 caagctgcag cgcgcccaga tcgatgaggt cgaagacaac ggcatggc
//
LOCUS       DASUGJ010000031         6053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_697949, whole genome shotgun sequence.
ACCESSION   DASUGJ010000031 DASUGJ010000000
VERSION     DASUGJ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697949"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1029)
                     /locus_tag="VFV93_00855"
     CDS             complement(88..1029)
                     /locus_tag="VFV93_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5163914.1"
                     /translation="MANQRTVDPSVRPDADGAATLDGGRATPLFEQRKVQRNRFMRRI
                     RAEKKAFLGIIFVVLLGIVALLGPQLAPHDPDNDDFNFLEAPSMSHPMGTDSYGRDLF
                     SRVIIGTRISFTVGILGALVSMVIGVTFGILSGYYLGWIDMVIMRVVDLLWAFPAIIL
                     AMAFVAVYGAGARNVILAIGLGNLVAFARIVRGQVLSLREEDFTLAARAIGVRDWRIM
                     FLHLLPNVAAPIIVQMTLTVGFGILTETGLTFLGLGVDPATPTWGQALNESRSFIQQA
                     WWLAVFPGLAIGFTVLSLNLFGDGLRDALDVRSVGDD"
     gene            complement(1022..1966)
                     /locus_tag="VFV93_00860"
     CDS             complement(1022..1966)
                     /locus_tag="VFV93_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5163915.1"
                     /translation="MTRYIFRRLLYAVFIIWGCATIVFFLLRLVPGDPVSVMLGAEYT
                     PEAAAQLRENLGLNEPVYTQYVKWMGNLLQGDLGKSIATKEPVTHAIQRALPKTLSIT
                     ALGFLIGVVIAVPLGMLAALRRGSIFDFLASSSTFVGISLPTFWFGIVFILIFAVKLN
                     WLPSVGYVSPSDGLWPWFEHLILPGLAAGVGEAAILMRFVRAGLLDVLGSDYIRTARA
                     KGLNERRVILGHAMRTALIPVVTVAGLSLAGLLGGLVITETVFSISGIGRLLTSAIFS
                     KDYPIVQGVILLITMIFVLANLAVDVVYTVLDPRIRYG"
     gene            complement(2059..3852)
                     /locus_tag="VFV93_00865"
     CDS             complement(2059..3852)
                     /locus_tag="VFV93_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5163916.1"
                     /translation="MTQEDAFTLAKLTERVATRRSVLKFAVATAGTLGVTGLLAACGG
                     DDDDDDDDGGTTPAATSGTSSEPTATTAASGGASPETTASAGTSDEPTATAAAGAPTT
                     ATGGGEGKQGGTLTLGFNVQQLLQLDPARISTGRVAGQLSCNLSSALVQFDETLAIQP
                     DLAESWEVSDDGLVYTFHLRDGLTFHNGDPLKAEDFVYTFNRTQDEALASPHANKLAN
                     INKAEAVDELTFEMTFNDPFGPFLAVACSRGPGRALAPIPKRAVDEIGEDEFLLKPVG
                     SGPFMLVPDTMDPQGGFEMVAFENWYGGRPFLDKIVVKFIPEPASQANALAAGDIDMI
                     NEVPAQGWDQLEGADGVVLEQSPGTNWIGLQFNTTRPPWDQIDARLAIAKAINKDEFV
                     EKARFGLADPSHGAIAPAFAWAFVPLDQLEENPQAYDLDEAKSMAEATGVLDAKPKLL
                     TGAADDRNEQQIRNQLQDLGIDVELDKVEDAVYNDRWQNGDYDWAINGSVVDADPDDN
                     DFNFFYPDGPWNTGKWNNDQAKQLLDAERATSDQDARAKAFQDLMYLTLKEAPLAFLY
                     HQFDMVGYRDFVKDYVKIPEMRYLERVWLDK"
     gene            complement(3971..4756)
                     /locus_tag="VFV93_00870"
     CDS             complement(3971..4756)
                     /locus_tag="VFV93_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX5163917.1"
                     /translation="MSDPLAIVPIRRSRISQQIVVQLCQMIRQGQLRPGDRLPPEREL
                     SEQLKVSRASLREALRALEIAGIVAAKQGGGTYIREVFEDEILSPLSLVLDASTDLVG
                     DLWEVRSIFEPAVASRAAIRAQAADIAVLERIVEQQHGFLDRDAANDDWLESDRAFHV
                     AVARASHNEVSVRVIRLINELLHEGRRHFATRSDRREHAYTWHREIAARIRACEPQQA
                     RDAMMQHLREVEEVIVSEVIAPITGDQAELEETMTSPNPTAAS"
     gene            complement(5166..5537)
                     /locus_tag="VFV93_00875"
     CDS             complement(5166..5537)
                     /locus_tag="VFV93_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5163918.1"
                     /translation="MSTSATFGFVVEYVADIEATRRYLVETLGLTVAREAPTFVQFTD
                     SNGASFAIASDESMTGARDPELYWLVDDAEATHRDLATRTDITLPLTQKPFGTVFGVT
                     DPAGQPHYLLELARNRPSRAV"
     gene            complement(5627..>6053)
                     /locus_tag="VFV93_00880"
     CDS             complement(5627..>6053)
                     /locus_tag="VFV93_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX5163919.1"
                     /translation="AGRSVLTKLAGVMGAAPRETAGRLLDRTRVVSGPVGPTGDLAGN
                     IQQALLDGVAVTLAYRDASGVETQRVVEPAGVFATPNGWYLAAWCRLRRAPRAFRLDR
                     IDAVTLSNEPVAPRSLEAMLAELPYELTEPAFAECEAPQ"
BASE COUNT         1063 a         1885 c         2025 g         1080 t
ORIGIN      
        1 tcgaacgtcg cgtcgattca cacgcaaatg atccccgtga ggtgtggcag ggatgcgctg
       61 gtcgggcgca tccctgccac gttgcggcta gtcgtcgccg acgctgcgga cgtcgagggc
      121 gtcgcgaagg ccgtcgccga agagattgag gctgagaacg gtgaagccga tcgcgaggcc
      181 ggggaagacg gccagccacc aggcttgctg gataaagctg cggctctcgt tgagcgcctg
      241 gccccaggtc ggtgtggccg ggtcgacgcc gaggcccagg aacgtcaggc cggtctcggt
      301 caggatgccg aagccgacgg tgagcgtcat ctggacgatg atcggcgcgg caacgttcgg
      361 aagaagatgg aggaacatga tgcgccagtc gcgcacaccg atggcgcggg ccgcgagcgt
      421 gaagtcctct tcacgaaggc tgaggacttg cccacggacg atccgcgcga acgcgaccag
      481 attcccgagg ccgatcgcga gaatgacatt tctagcgccg gcgccgtaga cggcgacgaa
      541 cgccatcgcc aggatgatgg ctggaaacgc ccagaggaga tcgaccacgc gcatgatgac
      601 catatcaatc cagccgagat aatagccgga cagaatgccg aacgtgaccc cgatcaccat
      661 cgagaccagc gcgccgagga tgccgacggt gaacgagata cgcgtcccga tgatgacccg
      721 gctgaacagg tcgcgtccgt aggagtcggt acccatcgga tgactcatgc tcggcgcttc
      781 gagaaagttg aaatcgtcgt tgtcgggatc atgcggcgcg agttgcggcc ccaggagcgc
      841 cacgatgccg agaagcacga cgaagatgat ccccaggaac gccttcttct cggccctgat
      901 gcggcgcatg aagcggttgc gctggacctt gcgctgctcg aacagtggcg tggcgcgccc
      961 gccatccagc gttgcggctc cgtccgcatc gggacgaact gagggatcga cagtgcgctg
     1021 attagccata gcgaatcctc ggatccagga ccgtatagac gacgtcgacg gccaggttag
     1081 ccagaacgaa gatcatcgtg atcagcagga tcactccctg cacgatcgga tagtccttgc
     1141 tgaagatggc cgacgtgagt agccggccga tgccgctgat cgagaagacg gtctcggtga
     1201 tcacgaggcc accgaggagt ccggccagcg acagtccggc gacggtgacg acggggatca
     1261 acgccgtgcg catcgcgtgg cccaggatga cgcgccgttc gttcagaccc tttgcccgcg
     1321 cggtgcggat gtagtcactg ccaagcacgt cgagcaggcc ggcacgcacg aagcgcatga
     1381 ggatggccgc ctcgccgacg ccggccgcca ggcccggcag gatcaggtgc tcgaaccagg
     1441 gccagaggcc atccgacggc gacacgtagc cgaccgacgg cagccagttg agcttgacgg
     1501 cgaagatcag gatgaagacg atcccgaacc agaacgtggg caggctgata ccgacgaacg
     1561 tcgaggaact ggccaggaag tcgaagatgc ttccgcgtct cagcgccgcc agcataccca
     1621 acggcacggc gatgacgacc ccgatcagaa agcccagcgc cgtgatgctc agtgtcttgg
     1681 ggagggcgcg ttggatcgcg tgggtgacag gctccttggt cgcaatcgac ttgccgagat
     1741 ccccctgaag caggttgccc atccacttga cgtactgcgt gtagaccggc tcgttcaacc
     1801 ccaggttctc gcgcagctgc gcggccgctt cgggcgtgta ctcggcgccg agcatgaccg
     1861 agaccgggtc gcccggcacg agccgcagca aaaagaagac gatggtcgcg catccccaga
     1921 tgatgaagac cgcgtacagc agtcggcgga agatgtaccg tgtcacgctg tcctccggtt
     1981 cctggactag agaccggggg agcgagacga cgcatcaggg ctgatgcatc gctcgcgccc
     2041 catccccggc ccccgttgct atttgtccag ccagacgcgt tccaggtagc gcatctccgg
     2101 gatcttgacg tagtccttga cgaagtcccg atagccgacc atatcgaact ggtggtagag
     2161 gaaggccagt ggcgcttcct tcagcgtgag atacatcaga tcctggaacg ccttggcgcg
     2221 cgcgtcctgg tctgacgtag cgcgctcggc gtcaagcagt tgcttggcct ggtcgttatt
     2281 ccacttgccg gtgttccacg ggccatccgg atagaagaag ttgaagtcgt tgtcgtcggg
     2341 gtcggcgtca acgacgctcc cgttgatggc ccagtcgtag tcgccgtttt gccagcgatc
     2401 gttgtagacg gcgtcttcga ccttgtcgag ctcgacatcg atacccagat cctgcaactg
     2461 attgcggatc tgctgctcgt tgcggtcgtc ggcggcgccg gtcagcaact tcggcttcgc
     2521 gtcaaggaca ccggttgcct ccgccatcga cttggcctcg tccaggtcgt acgcctgcgg
     2581 gttctcttca agctgatcga gcggcacgaa cgcccaggcg aaggccggcg cgatcgcgcc
     2641 gtggcttggg tcagcgaggc cgaatcgagc cttctcgacg aactcgtcct tgttgatcgc
     2701 cttggcaatc gccaaccgcg cgtcgatctg gtcccacggc ggtcgggttg tgttgaactg
     2761 gaggccgatc cagttcgttc ctggtgactg ctcgaggacg acgccgtcgg cgccttccag
     2821 ctggtcccag ccctgggcgg gtacctcgtt gatcatgtcg atgtcaccgg ccgccagcgc
     2881 gttggcctgg ctggcaggct cggggatgaa cttgacgacg atcttgtcga gaaacggtcg
     2941 cccgccatac cagttctcga aggcgaccat ctcgaaaccg ccctgcgggt ccatcgtgtc
     3001 ggggacgagc atgaatgggc cggagccgac cggcttgagc aggaactcgt cctcaccgat
     3061 ctcatcgacg gcccgcttgg ggatcggagc cagcgcccga cccgggccac ggctgcaggc
     3121 gacggccagg aatgggccaa atggatcgtt gaaggtcatc tcgaaggtca actcatcgac
     3181 cgcttcggcc ttgttgatgt tcgcaagctt gttggcatgc ggggatgcca gagcctcatc
     3241 ctgggtgcgg ttgaaggtgt agacaaagtc ttctgccttc agcggatcgc cgttgtggaa
     3301 tgtcagccca tcgcgcagat ggaacgtgta gacgaggccg tcgtcggaca cttcccagct
     3361 ctcggccagg tccggctgaa tcgccagtgt ctcgtcgaac tggacgagcg ccgaggacag
     3421 gttgcaactg agctggccgg ccacacggcc ggtgctgatc cgcgccggat cgagttggag
     3481 caactgctgg acgttgaacc ccaacgtcag cgtgccgccc tgcttgccct cgccgccacc
     3541 ggtcgccgtc gtcggcgcgc cggctgcggc tgttgcggtc ggctcgtccg acgtgccggc
     3601 cgaggcagtg gtctccggcg atgccccgcc gctggccgcg gtcgtggctg ttggctcgct
     3661 actggtacca ctggttgcgg ctggtgtcgt gccgccatcg tcatcgtcgt catcatcatc
     3721 gccaccgcag gccgcgagca ggccggtgac gccaagcgtg ccggcagtcg caactgcgaa
     3781 cttgagcacc gagcggcgcg ttgccacccg ctccgtcagt ttagcgaggg taaatgcatc
     3841 ctcctgagtc acgtttctcc tccttatcgg cctgtcgaat acaggtcctg ccgttggcgt
     3901 gacatcccac ccggattctc cccctacgtc gctggcgacc aatcctccct tcttcgtctg
     3961 tcaactacgg ttagctggcc gcagtcggat ttggtgacgt catcgtctcc tccagttcgg
     4021 cctgatcgcc cgttatgggt gcgatgacct cactgacgat gacttcttcg acttcgcgca
     4081 ggtgctgcat catcgcgtca cgcgcctgct gcggttcgca ggcacgtatc cgcgcggcaa
     4141 tctccctgtg ccaggtatac gcatgctcac ggcgatccga ccgtgttgcg aagtgccggc
     4201 gaccctcatg caggagctcg ttgatgaggc ggatgacgcg aaccgagact tcattgtgcg
     4261 aagcgcgggc aaccgcgaca tgaaaggcgc ggtcactctc cagccagtca tcgttggccg
     4321 catcgcgatc gaggaaaccg tgctgctgct cgacaatccg ctccagcacg gcgatgtcgg
     4381 ccgcctgagc acgaatggcc gcgcgcgagg ccaccgccgg ctcgaagatc gagcgaacct
     4441 cccagagatc accgaccaga tcggtgctgg cgtcgaggac gagcgacagc ggtgagagaa
     4501 tctcgtcctc gaacacttcc cgaatgtagg tgccaccacc ctgcttggcg gccacgatgc
     4561 cggcgatctc cagcgcccgc aaagcctccc gcagcgacgc tcgactgacc ttgagctgtt
     4621 cggagagctc gcgctcgggc ggcagccggt cacctgggcg cagttgccct tgccggatca
     4681 tctggcaaag ctggacaacg atctgctgtg aaatccgtga tcgccggatt gggacgatcg
     4741 cgaggggatc gctcactggt caccgcgtaa cgatttgtca gatggtcaga ccaattggct
     4801 atgcgggtac tgtaagggtg gatgggcttg atgtcaatgg ctgaggggtt tcgggacgcg
     4861 ggacgtggga cgtgggacgt gggacgtggg accaggtgaa cagcgtcaac gaggaacagc
     4921 gcccctcacc cctgtcatcc tgagccgcag cgaaggatcg cctggcgtcg atggtgtgag
     4981 cggttcgggc gggccgacgg cggtctgccg attggcgctc gcatgtcttt acggctcagc
     5041 gatccttcgc tgcggctcag gatgacaggg gtgaggggcg ctgttcctca gtgacgccgt
     5101 tcatctggtc ccgcatcccg cgtcccgcgt cccacgtccc gcgtcccgcg tcccgcgccc
     5161 cgtcgtcaca ccgcgcggct ggggcggttg cgggccagtt cgaggaggta gtggggctgg
     5221 ccggccggat cggtgacgcc gaagacggtg ccgaacggct tctgggtcag cgggagggtg
     5281 atgtcggttc gcgtggcgag gtcgcggtgg gtggcttcgg cgtcatccac gagccagtac
     5341 agctcggggt cgcgggcgcc ggtcattgat tcgtcgctgg cgatcgcgaa cgatgcgccg
     5401 ttggagtcgg tgaactggac gaacgttggg gcctcacgcg cgacggtgag gccaagtgtc
     5461 tcgacgagat aacgtctggt tgcctcaata tctgccacgt actcgacgac gaatccgaag
     5521 gttgcgctgg tgctcatgac tggctgctcc tttgcaggtt cccgtggcgg tcactccgcc
     5581 acgatcaaca gcttagtcac aggtggcgac aacagtatgt cgggaattac tggggtgcct
     5641 cgcactcggc gaaggctggt tcggtcagct cgtagggcag ttcggccagc atcgcctcca
     5701 gcgagcgggg cgcgaccggc tcgttactga gtgtgacggc gtcgatgcgg tccagccgga
     5761 aggcacgcgg agcgcggcgc aggcggcacc aggcagccag ataccagccg ttcggggtgg
     5821 cgaagacgcc ggccggctcg acgacgcgct gggtctcgac accgcttgcg tcacggtagg
     5881 cgagcgtgac ggcgacgccg tcgagcaagg cttgctggat gttgccggcc aggtctcccg
     5941 ttggtccaac cggcccgctg acgacgcggg tgcggtcgag cagccggccg gccgtctcac
     6001 gcggcgcggc gcccatcaca ccggccagct tggtgaggac cgagcggccg gcg
//
LOCUS       DASUGJ010000032         3813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1393406, whole genome shotgun sequence.
ACCESSION   DASUGJ010000032 DASUGJ010000000
VERSION     DASUGJ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393406"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VFV93_00885"
     CDS             complement(<1..942)
                     /locus_tag="VFV93_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5163920.1"
                     /translation="MTDRVPRSFSGPVRHQLFGRSREHALLREVLARMLAGDGHLVLV
                     GGEAGIGKSALLRLLAHEAAQQGVVVLTGLCYDVTTPHPYGPWVELARRAGPAGSLAP
                     FPAFLHDIDAVATLGSQERLFREVTACFTTVAAQQPLVLLLEDLHWADQGSLDLLRYV
                     GRDVVGQRLLLVASYRDDELTRRHPLFQLLPTLIRESEATRLVLARLDADAMRALVRA
                     RYALTEPDEHRLHRYIQGLTEGNPFFIHEVLHALEDNGALARVDDSWQLGDLSRIYLP
                     TLVREVIERRLAGIDDDARRYLEMAAVIGQDVPLDLFM"
     gene            1043..1201
                     /locus_tag="VFV93_00890"
     CDS             1043..1201
                     /locus_tag="VFV93_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163921.1"
                     /translation="MRRRRGCLGTAAQLVGDPDLLVTATAEFNERADGFTYKAAIPEG
                     AELPKPHI"
     gene            1428..2531
                     /locus_tag="VFV93_00895"
     CDS             1428..2531
                     /locus_tag="VFV93_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5163922.1"
                     /translation="MIGLLEIRRRKTQFALIASVFVLIAYLVMMINGLGIGLNQLAGS
                     ALLNMDADAIAYSSDAGRSVIRSELSQETLDRIQTAPGVEESAPLVYTAASYERSSGK
                     ISSAALLGYDPGTIAEPPVTAGRSLTEEDHNGVLADATFLDDSGLAIGDTLPLRLRLQ
                     TVELTIVGEIDEGAFFFQPAIYLLRSTLLDLKYGPATGGERPVASIALTQGDDSIRSG
                     DGFELVDKQTAFDNIEGVSGQQATVTSLQAFGYIIGAMIIGVFFYVLTLQKIPQFGVL
                     KALGASNRYIIGQLLTQVLVIAGIGIGLSLLLALVTERGLQSLPDTVPIAFTSRMFII
                     TCVALLVTALIGALFSARQVLRIDPIIALGQQQ"
     gene            2574..3245
                     /locus_tag="VFV93_00900"
     CDS             2574..3245
                     /locus_tag="VFV93_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5163923.1"
                     /translation="MLQIANLTKTYGSSEQAVHAVQDVSLSAQAGEFLAVIGPSGSGK
                     TTLLAMIGGLLTPTSGAISLAGRDIARMSSGQLAAFRRQSVGYVFQANNLLPYLTARE
                     NLLVMEAIGARRGAGKQRADQLLHELGMDARANAVGTELSGGERQRVAIARALMNDPE
                     LVLVDEPTASLDSSRGKQVVQLLIDETKARSKLAIMVTHDLDMAIMADRILEMRDGVL
                     REQAG"
BASE COUNT          729 a         1238 c         1174 g          672 t
ORIGIN      
        1 catgaacagg tcgagcggaa catcctgccc gatcacggcc gccatctcga ggtagcgtcg
       61 cgcgtcatcg tcgatgcctg ccagccggcg ctcgatcacc tcccggacca gcgtcggcag
      121 gtagatacgg ctcaggtcgc caagctgcca gctatcgtcc actcgcgcca gcgcgccgtt
      181 gtcctccaac gcatgcagca cttcatgaat gaagaaagga ttgccctccg tcagcccctg
      241 aatgtaacgg tggagccggt gctcatccgg ctcggtgagc gcgtaccgtg ccctgacaag
      301 cgcacgcatg gcgtcagcat cgagtcgcgc aagaacgagc cgcgtcgctt ccgactcgcg
      361 gatcaacgtc gggagcagct ggaacagcgg atggcgacgg gtgagttcgt catcgcggta
      421 gctggcgacc agcagcagcc gctgccccac gacgtcgcgc ccgacgtacc gcagcagatc
      481 gaggctaccc tgatctgccc agtgcagatc ttcaagcagc agcaccaacg gttgctgggc
      541 ggcgaccgtt gtgaagcagg ccgtgacctc gcggaagagg cgctcctggc tgccaagcgt
      601 ggccacggcg tcaatgtcgt gcagaaacgc gggaaaggga gcgagcgatc ctgccggccc
      661 ggcccggcga gcgagctcaa cccagggacc gtacggatga ggcgtcgtca cgtcgtagca
      721 caatccggtc aggacgacga caccctgctg ggcagcctcg tgcgccagca gccggaggag
      781 agcggacttg ccgatgccgg cctcgccacc gacgagcacc aggtgtccat cgcccgccag
      841 catcctcgcc agcacctcgc gcagcagcgc gtgctcgcgg gaccgaccaa acagctgatg
      901 gcgaaccggt ccagagaagg acctgggcac tcgatcggtc atggacgaca cctcgcgact
      961 gacaccctcg gcatcgtgat cgcgaccagt tcgtctcagt atagcgccgg tcaccgccct
     1021 cgagtgcggc gagaagcgtg gcatgcgccg ccgtcgagga tgtctcggaa ccgccgctca
     1081 actcgtcggt gatcccgacc tcctggtcac ggccacggcc gagttcaacg agcgcgccga
     1141 cggcttcacc tacaaggccg ccatcccgga aggcgccgag ctgccgaagc cgcacatcta
     1201 gtcgtttcac ccccggaatc tcgtcgtgta accgccgtgt cgtggaatgg tgacgcgacg
     1261 agattctcgt atgagaatca acgcgtcacg caatggaacc ggggttgcgt gggtatgatt
     1321 cggcgcaatc tgatgaacat ggccggagcg tacgccgggg aatcgccggg gcacattcga
     1381 cgccatctgc acccacctga accggacggt ccgcgatggg ggataccgtg ataggcctgc
     1441 ttgaaatccg ccgacgcaag acgcagtttg cgctgattgc cagcgtgttt gtcctgattg
     1501 cctacctcgt gatgatgatc aacggtctcg gtatcgggct gaaccagctc gccggcagcg
     1561 cgctcctcaa tatggacgcc gatgcgatcg cctattccag cgacgccgga cgtagcgtca
     1621 tccgctcgga actcagtcag gaaacgctcg atcgcatcca gacagcgcct ggcgtcgaag
     1681 aatccgcccc acttgtgtat accgccgcaa gttatgagcg ctcgtcgggc aagatcagtt
     1741 cggctgcatt gctgggctac gatccgggaa caattgccga gccaccagtg acggctggcc
     1801 gctcgctgac ggaggaggac cacaacggcg tcctcgcaga cgccaccttc ctcgatgact
     1861 caggtctggc catcggagat acgctaccgt tgcgcctgcg ccttcagacg gtcgagttga
     1921 cgatcgtcgg agagatcgac gagggtgcct tcttctttca gccggccatc tacctgttga
     1981 ggtcgacgct gctcgatttg aaatacggtc cggccaccgg tggagagcgg cctgtcgcca
     2041 gcatcgcgct cacccaggga gacgattcca tcaggtccgg cgacggcttc gaactggtcg
     2101 acaagcagac cgccttcgac aacatcgagg gggtctcagg acagcaggcg accgtcacat
     2161 cactacaggc gttcggctac attatcggag ccatgatcat cggcgtcttc ttctatgttt
     2221 tgacgctcca gaagatcccg cagttcgggg tgctcaaagc gctcggggca agcaaccgct
     2281 acatcatcgg ccagctactc acccaggtgc ttgtgattgc cgggatcggc atcggcctgt
     2341 cgctgctgct ggcgctcgtg actgaacggg gcttgcaatc actacccgac acggtcccga
     2401 ttgcgtttac ttccagaatg ttcattatca cctgtgtcgc cctgcttgtc acagccctga
     2461 tcggcgcact cttcagcgcg cgtcaggtgt tgcgtatcga tcccatcatt gccctcgggc
     2521 agcagcagta ggaacggatc cgagcgaggg agtcgcggca cgacatgtcc gagttgttgc
     2581 agatcgccaa tctcacgaag acgtacggca gcagcgagca agccgtccat gccgtccagg
     2641 acgtctcact gtctgctcag gccggtgagt ttctggccgt catcggcccg agcggctcgg
     2701 gcaagaccac cctgctcgcg atgattggcg gcctgctgac cccgaccagc ggcgcgatct
     2761 cactggctgg ccgcgacatc gcgcgcatgt cgtccggcca gcttgccgcg tttcgccgcc
     2821 agtctgttgg gtatgtcttc caggccaaca atctgctgcc atacctgaca gcgcgcgaga
     2881 acctgctcgt gatggaggcg atcggtgcac gccgaggcgc cggcaagcag cgcgccgacc
     2941 agctgttgca cgagcttggc atggatgcgc gtgccaacgc cgtcgggacg gaactgagcg
     3001 gcggcgagcg ccaacgcgtg gcgatcgcgc gcgcactcat gaatgatcct gaactggtgc
     3061 tggtcgatga acctacggcg agcctggact catcccgtgg caaacaagtc gtgcaattgc
     3121 tgatagacga gaccaaggcg cgcagcaagc tcgcgatcat ggtaacgcac gacctcgaca
     3181 tggccattat ggcagaccgt atcctggaaa tgcgcgacgg cgtcctgcgc gagcaggcag
     3241 gctagcggca gcaaggtcat caactcccaa cctttgtgta cggcatcatc gtctcgctgt
     3301 tcctgccctt caacagcttc gccatcaaca tggtgccgca atcgactggc atgtcggtca
     3361 actgctcggc acgctcggcc tcagatcgcg cgatgaggtg acggtcccgt tgatcgagca
     3421 cggaccgatg ccggtcacca cctcgtaagc gctccccagg ctgcctgtcg ctgggaatac
     3481 gacgaagact ccgagaaatc gactccgcga tgatccacct ggaggctgcc tcttccgtca
     3541 tcctgtgcga tgccaatggc tgaccactgt cagcgctggg cggcctgttt cgctgtcgcg
     3601 ctcagtccgg cgacgatgtc gcgcatgatc acccggctcg gctggacgcg aaaaaatgga
     3661 cagtggcagc ccgcgagcgc gatgaatcgg cccgtgctgc ctggtggagc gaaacgctga
     3721 agctgtcggc ggcggagctg gtcttcctca atgagacggg caccaatacg gccatgtccc
     3781 aacgctatgc gcgggcccca cgtggacaac ggg
//
LOCUS       DASUGJ010000033         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1115628, whole genome shotgun sequence.
ACCESSION   DASUGJ010000033 DASUGJ010000000
VERSION     DASUGJ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115628"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1906
                     /locus_tag="VFV93_00905"
     CDS             149..1906
                     /locus_tag="VFV93_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5163924.1"
                     /translation="MQTSNAGTLPLLKRFFGYYRPHRRLFALDFSCAVLSGVLELAFP
                     MAVGLFVDRLLPGENWGLIILYAIGLLTVYLINTGLMVIVGYWGHMLGINIETEMRRK
                     SFDHLQKLSFRFYDNNKTGHLVAKVTKDLEEIGEVAHHGPEDIFVAIMTFIGAFALML
                     WVYPPLALIAAFVVPAAGWITARYGSRMTATWKLLFRRVGEFNARIEDNVGGIRVVQA
                     FANEDHERELFAENNAQYRSTKLEAYRIMTASMSLSYFSMRLAQIIVMIAGAYFVLHD
                     RLTEGEFVTFLLLINVFFRPIEKINQVLESYPKGIAGFRSYTSLLDTEPDIQDAPDAI
                     TVDGLRGDIRFENVTFGYGPEHTVLSDINLRIHAGETVAFVGPSGAGKTTIASLLPRF
                     YEIDAGRITIDGIDIQQMTLASLRREIGVVQQDVFLFTGTIRENIIYGRLDATEEEIA
                     AAVRRARMDTVVAGMPDGLDTVIGERGVKLSGGQKQRLAIARMFVKNPPILILDEATS
                     ALDSETERAIQASLTELAKGRTTLVIAHRLATIQNADRIVVVDETGIAEEGRHQELLK
                     TEGLYQRLHAAQFASYAAS"
BASE COUNT          408 a          730 c          694 g          382 t
ORIGIN      
        1 ggagcagggc cggcacaaga ccggccccta cgtcgtgcgg gcatgacggt acaatgatcg
       61 atggaccgtt tcgagacgga gttcggcaca gcgcgctggc ggtgtgccgc atcagtgtcc
      121 atccactcac gacacagaga aagcacgtat gcagaccagt aacgcgggga cactcccgct
      181 tctgaagcgc ttcttcggct actaccggcc gcaccgcagg ctgttcgcgc tggatttctc
      241 ctgcgcggtc ctctccggtg tgcttgagct ggccttcccg atggcggtcg gactgttcgt
      301 cgaccggctc ctgccgggag agaactgggg attgatcatc ctctacgcga tcggcctgct
      361 gaccgtctac ctgatcaaca ccggcctgat ggtcatcgtc ggctactggg gccatatgct
      421 cgggatcaac atcgagacgg agatgcgccg caagagcttc gaccacctcc agaagctctc
      481 gttccgcttc tacgacaaca acaagaccgg ccacctggtc gccaaggtga cgaaggatct
      541 ggaagagatc ggcgaggtcg cccaccacgg gccggaggac atcttcgtcg cgatcatgac
      601 ctttatcggc gccttcgcgc tgatgctctg ggtctacccg ccactggccc tgatcgcggc
      661 cttcgtcgtc ccggccgccg gctggatcac cgcccgctac ggttcgcgga tgacggcgac
      721 ctggaagctg ctcttccgac gggtcggcga gttcaacgcg cggatcgagg acaacgttgg
      781 cgggatccgc gtcgtccagg cgttcgccaa cgaggaccac gagcgagaac tgttcgccga
      841 gaacaacgcc cagtaccgat cgaccaagct ggaggcctac cggatcatga cggccagcat
      901 gtcgctgagc tacttcagca tgcggctggc ccagatcatc gtcatgatcg ccggcgccta
      961 cttcgtcctg catgaccggc tgacggaggg cgagttcgtc accttcctgc tgctcatcaa
     1021 cgtcttcttt cgcccgatcg agaagatcaa ccaggttctg gagagctacc cgaaggggat
     1081 cgccggcttc cgcagctaca ccagcctgct cgacaccgag ccggatattc aggacgcgcc
     1141 cgacgccatc acggtcgacg gcctgcgcgg cgatatccgc ttcgagaacg tcaccttcgg
     1201 ctacggcccg gagcacacgg tcctcagcga catcaacctg cgcatccacg ccggtgagac
     1261 ggtcgccttc gtcggtccat ccggcgccgg aaagacgacg atcgcctcgc tactgccgcg
     1321 cttctacgag atcgacgccg gccggatcac gatcgacggg atcgatatcc agcagatgac
     1381 gctggcctcg ctgcggcgcg aaatcggcgt cgtccagcag gacgtcttcc tcttcaccgg
     1441 cacgatccgc gagaacatca tctatggccg gctcgacgcg accgaggagg agatcgcggc
     1501 agccgtccgc cgggcgcgga tggacaccgt cgtcgccggc atgccggacg gactcgacac
     1561 cgtcatcggc gagcggggtg tcaagctctc cggcggccag aagcagcggc tggcgatcgc
     1621 gcgaatgttc gtcaagaacc cgccgatcct gatcctcgac gaggcgacct cggcgctgga
     1681 ctccgagacg gaacgagcga tccaggcatc gctgacggag ctggccaagg ggaggacgac
     1741 gctcgtcatc gcccaccgcc tcgccacgat ccagaacgcc gaccggatcg tcgtcgtcga
     1801 cgagaccggc atcgccgaag agggccgcca ccaggaactg ctcaagaccg aggggctgta
     1861 ccaacgcctg catgccgccc agttcgcgtc gtatgcggcg agttgacatg tcgtgatgtt
     1921 tctggatcgc tgaccgaatc cagggtcgcg tgatcgaatc gcatcgtctc cacaggcaac
     1981 aaacgacacc ccccgtgctg gggtgtcgtt gtcgtcgttc gtgcatgtgc gcggtcgagc
     2041 agactgggac tcaggcgctg gcgctcatca tcgatggggc gggaacgaag ccggcgcaac
     2101 cgctgatcgg ggtcaccttc aggtcgagcg cggcgtgctg cgggtgacca cactggacga
     2161 cgaagttctc cggtgcctga tgcctggtgc ggatctggat caactgcggc tgat
//
LOCUS       DASUGJ010000034         3960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1254803, whole genome shotgun sequence.
ACCESSION   DASUGJ010000034 DASUGJ010000000
VERSION     DASUGJ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254803"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..648)
                     /locus_tag="VFV93_00910"
     CDS             complement(211..648)
                     /locus_tag="VFV93_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163925.1"
                     /translation="MQAVQVSEVSVERSSRLMMVAAGAALIGAASFALNIGDGLSGEI
                     PALGRALLGLVGLAGGALLWLRPRLGWNVALLWAIFQIPFIAWNTDGSVTSQLIDFPL
                     SMSSSTTVNGVVTSYSEFGINLIGVALVIVISRMRGSWMLRNR"
     gene            853..2268
                     /locus_tag="VFV93_00915"
     CDS             853..2268
                     /locus_tag="VFV93_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5163926.1"
                     /translation="MAIDANREMDRAILSEIWTSGESYNVLRELCDDIGHRFGGSESE
                     RKGAEFLKQKMQEYGLENVRIEEFKMAGWDRGSAYLKLVEPVEREFPVIAMPYCPSAD
                     ITAEIIDVGEGELADFERLGDQVKGKIVISAAETNKPGERKSHRTDKFGWAIERGALA
                     YIYINQNPGMLRITGSITGRNPGGTTAADREAPIPGVGVSWEAGSAILRLIERTGGKG
                     KIHLKLENRTYESTSRNVIGEIVGTEHPNEVILLGGHFDGHDISQGAGDDGAGTITGL
                     EAGRALAQFKGKLKRTVRIICFGSEEIGLLGAFHHAKTTDPDSYRFVMNLDGAGRGQG
                     GQEQLVLSGWPELGEWVKQWSQDHNYDLTVDDQLNSHSDHYPFALRGIPNGTLNSRDR
                     TAGLVGRGYGHTEADTFDKIHLRGLQMSAIAVACLALAMAQADDFPAKRRSENEVREQ
                     LKKNNLLERAERAGRFPAKQA"
     gene            2372..3781
                     /locus_tag="VFV93_00920"
     CDS             2372..3781
                     /locus_tag="VFV93_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5163927.1"
                     /translation="MSDEMKSIEQQILADIWTTDEPMTVLRELCDDIGHRFGGSESER
                     QGAEFLKQKMLDYGLENVRIEEFSLASWERGGASLKLLSPVERDFSVIAMPYCPAADL
                     TAEIVDVGEGELADYERLGRDAIQGRIVVTDAETNKPGERKSHRIVKYNWAVEYGAAA
                     IIYINQNPGMLHITGALFGHTSIHNPAEEWEVPIPGVGVTWEAGSAIRRLVERSDGEG
                     TVRLKLENRTFASTSRNVIGEIVGSEHPDEVVLFGGHFDGHDIAQGAGDDGAGTITGL
                     EAARVLAAHKGKLKRTARVICFGSEEIGLLGSFHHSQNTNPDSYRFVMNLDGAGRGQG
                     GQEQLTLTDWPELEPWFEKWSREHNYNFDIRQQVSAYSDHYPFALRGVPNGTVNSKDS
                     TAGMIGRGYGHTEADTLDKVHLRGLQMSAALVARLVASIANAEDWPEVERRTDEQVRE
                     MLEKNKFLAAAIKTGYLPG"
BASE COUNT          774 a         1268 c         1316 g          602 t
ORIGIN      
        1 gaccagccgc gctcccgaaa atgctgcaac gtcgtcggga ccgtcgaaag tgtgctgctc
       61 tcgtgcgaga ttccgccgat tgcgatccgc atggattcct cctcatcccc ttcaccaaac
      121 cggccggcgg catgatagcg cgataaatgc cggaaacaga acgacccgct ggcgatacca
      181 gcgggtcgtg tgatcgtcgt ggtggagaaa ctagcggtta cgcagcatcc aggatccgcg
      241 catgcgcgag ataacgatca cgagcgcgac gccgatcagg ttgatgccga actcgctgta
      301 ggaggtgacg acgccgttga cggttgtgct gctcgacatc gagagcggga agtcgatcag
      361 ttggctggtg acgctgccgt cggtgttcca ggcgatgaac gggatctgga agatcgccca
      421 gagcagcgcg acgttccagc cgaggcgtgg tcgcagccag agcagcgcgc cgccggccag
      481 gccgaccagc ccgagcaacg cacggcccag cgccggaatc tcgccgctga gaccgtcgcc
      541 gatgttgagc gcgaagctgg ccgcgccaat caacgccgca ccggcggcga ccatcatcaa
      601 gcgggatgaa cgttcgacgg agacctcgga tacctgaact gcctgcatgg atgagctcct
      661 tcttcgataa ctgcgggtga tcgacaacga tcaccaattc aatgcgcaca ttacagcgga
      721 atccggccaa tacgactgta aaggacgata cagcagccat gttgcgtcgc aggcgttggc
      781 ggctggctgg cgtcgtttcg ggacggtgct atgatggccg cctgtccggt ctgggaggaa
      841 gggagaacga cgatggctat cgacgcgaac cgggagatgg atcgcgcgat tctgagtgag
      901 atctggacca gcggcgagtc gtacaacgtc ctgcgcgagc tctgtgacga catcggccac
      961 cgctttggcg ggtccgagtc cgagcgcaaa ggagctgagt tcctcaagca gaagatgcaa
     1021 gagtacggcc tcgagaacgt ccgcatcgaa gagttcaaga tggccggctg ggatcgcggc
     1081 agcgcctacc tgaagctggt tgagccggtc gagcgcgagt tcccggttat cgcgatgcca
     1141 tattgcccat cggccgacat cacggctgaa atcatcgatg tcggtgaggg cgaactggcc
     1201 gatttcgagc gcctcggcga tcaggtcaag ggcaagatcg tcatctcggc cgccgagacg
     1261 aacaagcccg gcgagcgcaa gagccaccgg accgacaagt tcggctgggc gatcgagcgc
     1321 ggcgcgctgg cctacatcta catcaaccag aaccccggta tgctgcgcat caccggctcg
     1381 atcaccggcc gcaaccccgg cgggacgacc gccgccgacc gggaagcgcc gatccccggt
     1441 gttggcgtct cgtgggaggc cggatcggcc attctccgac tgatcgagcg caccggcggc
     1501 aagggcaaga tccacctcaa gctggagaat cgcacctacg agagcacgag ccgcaacgtg
     1561 atcggcgaaa tcgtcgggac cgagcatccg aacgaagtga tcctgctcgg cggccacttc
     1621 gacggccacg acatctcgca gggcgccggc gatgacggcg ccgggacgat caccggcctc
     1681 gaagccggcc gggcgctcgc ccaattcaag ggcaagctca agcggaccgt tcgcatcatc
     1741 tgcttcggct cagaggagat cggcctgctc ggcgctttcc accacgcgaa gacgaccgat
     1801 cccgactcgt accgcttcgt gatgaacctc gacggcgccg ggcggggtca gggcggccag
     1861 gaacaactgg tcctctccgg ctggccggaa ctgggcgagt gggtcaagca gtggtcgcag
     1921 gatcacaact acgacctgac cgtcgacgac cagctcaatt cgcactccga ccactacccg
     1981 ttcgcgctgc gcggtatccc gaacggcacg ctcaactcgc gcgaccggac ggccggcctg
     2041 gtcggacgcg gctacggcca caccgaagcc gacacgttcg acaagatcca cctgcgcggt
     2101 ctccagatgt ctgccatcgc cgtcgcctgc ctcgcgctgg cgatggctca ggccgacgat
     2161 ttccccgcca agcgccgcag cgagaatgag gtccgcgagc aactgaagaa gaacaacctg
     2221 ctggagcgcg ccgaacgcgc cggccgcttc ccggcgaagc aggcgtgatc gtcgggacgc
     2281 gggatgcggg atgcgggacg cgggacgcgg tagtgatccc aattcgcgtt ccgcgtcccg
     2341 catcccgcgt cccgacaacg aaggagcaac catgtccgac gagatgaaat cgatcgagca
     2401 gcagattctg gctgatatct ggacgactga tgagccgatg acggttctgc gcgagctgtg
     2461 cgacgacatc ggccaccgct tcggtgggtc ggagtcggag cggcaggggg ctgagttcct
     2521 caagcagaag atgcttgact acgggctgga gaacgtccgg atcgaggagt tctcgctggc
     2581 cagctgggag cggggcggcg cctcgctgaa gctgctctcg ccggtcgagc gcgacttctc
     2641 ggtcatcgcg atgccctact gcccggcagc cgatctgacg gccgagatcg ttgacgtcgg
     2701 tgagggtgag ctggccgact acgaacggct cggccgcgac gcgatccagg ggcggatcgt
     2761 cgttaccgac gccgaaacga acaagccggg cgagcgcaag agccaccgga tcgtcaagta
     2821 caactgggcc gtcgaatacg gcgcagcggc catcatctac atcaaccaga accccggcat
     2881 gctgcacatc accggcgccc tcttcggcca cacctcgatc cacaacccgg ccgaggagtg
     2941 ggaagtcccg atccccggcg tcggcgtgac ctgggaggcc ggctcggcca ttcgccggtt
     3001 ggtcgagcgc tcggacggcg agggaacggt ccggctcaag ctggagaacc ggacgttcgc
     3061 cagcacgagc cgcaatgtga tcggtgagat cgtcggctcg gagcatcccg acgaggtcgt
     3121 cctgttcggc ggtcacttcg acggccacga catcgcccag ggtgccggcg acgacggcgc
     3181 cggcacgatc accggcctgg aggcggcccg cgtactggcc gcacacaagg gcaagctgaa
     3241 gcggaccgcc cgtgtcatct gcttcggatc ggaggagatc gggctgctcg gctcgttcca
     3301 ccactctcag aacaccaatc cggactccta ccgcttcgtg atgaacctcg acggcgccgg
     3361 gcggggtcag ggcggtcagg agcagttgac actgaccgac tggccggaac tggagccgtg
     3421 gttcgagaag tggtcccgcg agcacaacta caacttcgac atccgccagc aggtgagcgc
     3481 ctactccgat cattacccgt tcgcgctgcg gggtgtcccg aacggcactg tcaactcgaa
     3541 ggactcgact gccggaatga tcgggcgcgg ctacggccac accgaagccg acacgctcga
     3601 caaggttcac ctgcgcggcc tccagatgtc ggcggcgctg gtggcccggc tggtggcctc
     3661 gatcgccaac gccgaagact ggccggaggt cgagcgccgc accgacgagc aggtgcgtga
     3721 gatgctggag aagaacaagt tcctggctgc cgcgatcaag accggctact tgccgggtta
     3781 gacgggacgc gggatgcggg acgcgggacg cggagggttc gacgcgacgt atctgatcgt
     3841 gtcgggcgct gccaccgcgc ggcgggtgcc tgagctgatc gggcaactcg cgccccgcgt
     3901 cccgaacctg ctgacggtgc tgacaccgaa cgcgcagcag atcgtcagcc ctcgcgaact
//
LOCUS       DASUGJ010000035         7811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_560225, whole genome shotgun sequence.
ACCESSION   DASUGJ010000035 DASUGJ010000000
VERSION     DASUGJ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560225"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VFV93_00925"
     CDS             complement(<1..705)
                     /locus_tag="VFV93_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163928.1"
                     /translation="MLHILSDETWVSGDTRTPLMSADIWFDPATNSGRVEVQPADDSG
                     LQAVTMIVADGSIIAVTFDEYSMEDSGVEDLTGCLGIDQSAVDFVWCGSFLGLISYSF
                     GAAGNELFDGPATPLIEGEFQGLPALIAEMQDPGFPEMDPVRWYIDRTTHLPLGMVAH
                     WSDDPDDTDTWTETVFTYVPDTADFDTDCLDPASLDVDGVHREPPAFGGDLTKVPVSL
                     GESFGPVGNIPQLDVFQ"
     gene            complement(1148..3748)
                     /locus_tag="VFV93_00930"
     CDS             complement(1148..3748)
                     /locus_tag="VFV93_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5163929.1"
                     /translation="MPARTLFHRPGREGRDDAPPMTERVLRASRELIAPTLNSELPAW
                     DRFSPLLLLAMLSTVPLVAVTSGTGSLFVAWLAVAYLLSGYLWVDHAVARADGSHYWR
                     AYVVAGSGLWAIALRLPASAGTTLTAIMGLASAGAIVAAVRLHQPTRREPWYLLAAAL
                     ALTELGHAGAAISIIRDGTTPAAAGLVSLPGMALLPVALWWLLYRRLRDSSRLVLLDS
                     AIVAAGLALLGWIAIAAPAVSDLAPSSIGLAVQLGWPLAGLAPLILTTRFLLTRWPDD
                     LPARQLIGAGTILLLAAIAASTASASGADAWSQHPALSLLWFPAMLAFGAAALHPSMT
                     RLAVAIHDQPAELTWQRWLLLTLALLMSPVGLLVQLQRTDEPVVVVLSGCGLVLGILY
                     ACRLAAVQRARVTSGAAARQLRLAANDLVVGGSQDDIIATTLAAARAIGGDGVTTWLY
                     LCDGTPGELICSGDPASGHSASRLIRERDLPRSARRVLAAGTSAMLRPDEISGLRAQL
                     GLPGDVAVVSIAPLCGNTELRGLLVSAGNRLTPDAARSQETLASLLALALDRLRLASE
                     LGYQPERVAVRARAADGTAFTFVDVDPADAASDEAVDDDPPVTTSLREAIENDELIAR
                     YQPRLDLVSGRITSVEMTAAWDSPLLGPIPRGALSAIAAEDGLSQPLRLAMLLRGCRE
                     AAGWQAAYPDRELEIVVPLDLAEAEAAGLAEDVGLALDETCCDPTRLVLQFTAATLLE
                     ASQPARNELTSIAAIGVQIAVGDAGASWSQLDQLSAIPISVIELEAAIVQRLESDQRA
                     ASIAGHLIARAHYDGARVVGHGVGRRDDWLRLRKLGCDLGQGNLFSAPLNADELATLL
                     NRAKERAATA"
     gene            4009..4269
                     /locus_tag="VFV93_00935"
     CDS             4009..4269
                     /locus_tag="VFV93_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163930.1"
                     /translation="MVETTIRLNDGLAAAVEAYLRDHPGTTGITELAEHLLEEFLASQ
                     GYVTHYVPLRIPIGPADSGEPADSAEHDRLFAEAWMSRRDRE"
     gene            4714..6468
                     /locus_tag="VFV93_00940"
     CDS             4714..6468
                     /locus_tag="VFV93_00940"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5163931.1"
                     /translation="MVADSVDHVASELRSAVSAERLQEHLNAFSTLFRDSGSEDEWKA
                     ARYIRDRMTEYGVRAEILEFDSLISWPREGTLTVLDADGTAGEEIPVRTRSFGTTTPA
                     GGIEAELVFVPFEQPEAGAMIFSHRSVAGDYSGLDVRGKIVLTMDGGPDGIRRAQERG
                     AAGHIHIWPSDEAVIHEMIGTSVWGTPTPESALRLPAIPVLGVTLTDGERLAEHCRAE
                     GVRVRMTSDVLTQWMRVPLVIADVEAGAAIDDRFLLVGAHIDSWYEGVTDNATGDAAL
                     IEMARVLHEQRSGLRHGVRFGWWPGHSTGRYSGSTWYADTRFIELRERCLGYLNIDSP
                     GVRETEVWDCRYNMGEIEHITSSVVRELSGQEPNIRRPLKAGDQSFLGVGLPSMGAFR
                     MLPQDHPDRKAVGGCGGAYWWHSPEDTLDKADANILAEDTRIYVTMAAEMCVPEAHPY
                     NFVPSANDFLTNLTALQEVAGQYLDLTPAIEAAQRYRAAAERLANQGAGSDVARYNAT
                     VLRVTRIVNPALFTIDGPYEMDPALQLPVLPGLASMRELATLDPASSEHRFLLTKLTR
                     QRNRIQDALDQAVRELGG"
     gene            6661..7350
                     /locus_tag="VFV93_00945"
     CDS             6661..7350
                     /locus_tag="VFV93_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5163932.1"
                     /translation="MVSGSVGGHLDEIPLFAELPPGMLAVLASESRVRRYPEGQVLFS
                     EGDPGDALLILEEGRVKVCRFMAGGQEIVLAVQEAPASFGELALVDGAPRSATVIAQT
                     AVVVRMLPRAALMGMIHNEPTVAMALMEGLVGMIRNTNDRLSDLQALDVPGRVAKWLL
                     GWAERSGQQRADGVAIPFEISQSDLAAELGTTRVSINKALRMFESLDVIASDKDEIVI
                     MKPERLQEYVY"
     gene            7397..>7811
                     /locus_tag="VFV93_00950"
     CDS             7397..>7811
                     /locus_tag="VFV93_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5163933.1"
                     /translation="MDSGPVELNGTRLYYEVSGPPDAPAVVLVHGFSLDTRMWAGQVG
                     PLSERYRVVRYDLRGFGRSAIPEAGVHYQHHDDLRALLDQLGIERATVVGLSLGGAVT
                     LDFALHHPQRLTGLVLADAVVPGFDAPDLADLSRQV"
BASE COUNT         1336 a         2465 c         2698 g         1312 t
ORIGIN      
        1 ctggaacacg tcgagttgtg ggatgttacc gaccgggccg aaggactctc cgagcgagac
       61 cggaaccttc gtcaggtcac cgccgaacgc gggtggttcg cgatggacgc catcgacgtc
      121 gagggacgcg ggatccagac aatcggtgtc gaaatccgca gtgtcaggga cgtaagtgaa
      181 caccgtttcc gtccaagtgt ccgtatcgtc tggatcatcc gaccagtggg cgaccatccc
      241 gagcggcaga tgcgttgtcc ggtcgatgta ccatcggact ggatccatct ccgggaagcc
      301 aggatcctgc atctcagcga tgagcgcagg gagtccctgg aattcgcctt caatcagcgg
      361 cgtggccggg ccgtcaaaga gttcgttgcc agcggcgcca aaagaatagc taatcaagcc
      421 cagaaagctg ccgcaccaga cgaagtccac tgcggactga tcgatcccaa ggcagccggt
      481 cagatcctcg acaccgctat cttccatcga atattcgtca aacgtgaccg cgatgataga
      541 cccgtcagcg acgatcatcg ttactgcctg aagtccgctg tcgtcggctg gttgcacttc
      601 aacccgtccg gaattcgtcg ccggatcgaa ccagatatcg gcagacatca gcggtgttcg
      661 ggtgtcgccg gatacccacg tttcatcgga gagaatgtgc agcatacctg gttgctcacg
      721 gagcgctcgc tcctggagtt cgaagaattt actccaaggt gagtcgcccg tagacgctgt
      781 tggagttgtg ctgatcgggc gggtcgccgt tgggctggct gtggcttgca tgagggcgag
      841 ggttggggtc ggttgttctg ttgtcggggt ggttttctcg gacgttggtg tgagatccgg
      901 tgtttgtgtg atgtccacaa ttggtgaggc cgatcctggt gctaactcct tcgcggttgg
      961 ctcgggactg ccgcaggcgg cgaggctcag gatgagcagc atcccggcaa tggtggcccc
     1021 caggagacgg cagcgatggc ggatcatagt acatctctta ctgtcgcgaa acgacctgcg
     1081 caaacaagta ttcgcgatgg ctgtagagcg cgccagcgtc cagtggtcct gttctgtgga
     1141 gcgggagtta agcggtcgcg gcgcgctcct tcgcgcggtt gagcagtgtg gcgagttcgt
     1201 cggcgttaag tggggctgag aagaggttgc cctggccgag gtcgcagccg agcttgcgga
     1261 ggcggagcca gtcgtcgcgg cggccgacgc cgtggccgac gacgcgggcg ccgtcgtagt
     1321 gcgcgcgggc gatcaggtgg ccggcgatcg aggcggcccg ctggtcgctc tcaagccgct
     1381 ggacgatggc ggcttcaagc tcgatgacgc tgatcgggat cgcgctcagt tgatccagtt
     1441 gcgaccagga ggcaccggcg tcgccaacgg cgatctggac gccaatcgcc gcgatcgacg
     1501 tgagttcgtt gcgggctggt tggctggctt ccagcagcgt tgcggccgtg aactggagga
     1561 cgagccgtgt tgggtcgcag cacgtctcgt cgagcgcgag cccgacatct tcggctagcc
     1621 cggccgcttc ggcctctgcc aggtcgagcg ggacgacgat ctccaactcg cggtccggat
     1681 acgctgcctg ccaaccggcc gcctctcgac agccgcgcag gagcatcgcg agccgcaacg
     1741 gctggctgag gccatcctcg gcggcgattg cggagagggc gccacgtggg atcggcccga
     1801 gcagcggcga atcccaggcg gccgtcatct cgacgctggt gatccgaccg ctcaccagat
     1861 cgagccgggg ctggtagcgg gcgatcagtt cgtcgttctc gatggcctca cgcagcgagg
     1921 tcgtgaccgg tgggtcgtca tcgacggcct catcgcttgc cgcgtcggca gggtcaacgt
     1981 cgacgaacgt gaacgcggtg ccatcagccg cccgcgcgcg aacggcgaca cgttccggtt
     2041 gatagccgag ctcgctggcg agccgcagcc ggtccagcgc gagcgccagc agcgaggcga
     2101 gcgtctcctg cgaacgcgca gcatccgggg tgagccggtt gccggcgctg acgagcaggc
     2161 cgcgcagttc tgtgttccca caaagcggcg cgatcgagac gacggcgaca tcgccgggca
     2221 gtcccagctg tgcccgcagc ccggagatct catcgggtct gagcatggcg gatgttccgg
     2281 ccgcgaggac gcggcgggcg ctacggggca ggtcgcgttc gcggatcagc cggctggcgg
     2341 agtggccgga ggcaggatcg ccggaacaga tcaactcgcc gggcgtgccg tcacacagat
     2401 agagccaggt cgtcacgcca tcgccaccga tggcgcgggc agcggcgaga gtcgtcgcga
     2461 tgatgtcgtc ctggctgcca ccgacgacca gatcgttcgc cgccagcctc aattgcctgg
     2521 ctgccgcgcc gctggtgaca cgggcgcgct gaacggccgc cagccggcag gcgtagagga
     2581 tgcccagcac gaggccgcag cccgagagga cgacaacgac cggttcatcg gttcgttgca
     2641 actggaccag cagaccgacc ggcgacatca acagggcgag ggtcagcagc agccagcgtt
     2701 gccaggtcaa ttcggccggc tgatcgtgga tcgccaccgc cagccgtgtc atcgatgggt
     2761 ggagcgcggc ggcgccgaac gccaacatgg ctgggaacca gagcaggctc agcgccggat
     2821 gctggctcca ggcatctgct ccgctcgcgg aggccgtcga ggcggcgatg gctgccagca
     2881 gcaggatcgt gccggcgccg atcagttgcc gggcggggag gtcgtcgggc cagcgggtca
     2941 gcaggaagcg tgtcgtcagg atgagtggcg ccagaccggc cagcggccag ccaagctgga
     3001 ccgccagccc gatgctcgat ggcgccaggt ccgagactgc gggcgcggcg atcgcgatcc
     3061 agcccagcaa cgctaaaccg gcagcgacga tcgccgagtc gagcagcacc agccgcgagc
     3121 tgtcgcgtag ccgccggtag agcaaccacc agagcgcaac cgggagcagc gccattccag
     3181 gcagcgagac cagcccggca gcggccggtg tcgtgccatc gcggatgatg gagattgccg
     3241 cgccggcatg gccgagttct gtcagggcga gcgctgccgc cagcaggtac cacggctctc
     3301 gccgcgtcgg ttgatgcagg cgaacggcgg cgacgatcgc gccggcgctg gccagcccca
     3361 tgatcgcggt gagggtcgtg ccggccgagg ccgggaggcg aagggcgatc gcccatagcc
     3421 cgcttccggc gaccacgtag gcccgccagt agtgcgaccc gtcagcgcgg gcgaccgcgt
     3481 ggtcgaccca cagatagccg ctgagcaggt aggcgacggc cagccaggcg acgaagagcg
     3541 aaccggtgcc ggatgtgacg gccaccaacg gcaccgtcga gagcatcgcc agcagcagca
     3601 gcggcgagaa gcggtcccag gcggggagct cgctgttgag cgttggcgcg ataagttccc
     3661 gcgatgcgcg caatacccgc tcggtcatcg gcggggcgtc gtcgcggcct tcacggcccg
     3721 gacgatggaa gagcgttctg gctggcatcg ttgtcgcgcg ctccacgaag tctggcgtgg
     3781 cctgtcgtgc cttcatgatc ctggcggccc cgcgcccgcc gccggcggga tgtgatcctt
     3841 caccaccaag cgtatgagat cgcctgcagc cgcgcgatgc gccaaacagc acccatttcg
     3901 ccgagaccaa tgtctatcga agggcggacg acagtaccgg tcgtttagga ctgcgatcgg
     3961 tggtgttaca cgcggattca gattggcgcg ttccggcagg agatcacgat ggttgagaca
     4021 actatccgcc tgaatgatgg gttagcagcc gcagtcgagg cgtatcttcg tgaccatccg
     4081 gggacgacag gtatcaccga gctcgccgaa catcttctcg aagagtttct tgcgtcccag
     4141 ggatacgtga cgcactatgt gccgttgcga attcccatcg gaccggccga ctctggcgaa
     4201 ccggccgaca gcgctgaaca cgatcggtta tttgcagaag catggatgtc gcgccgcgac
     4261 cgagaatagt gctggccgat acgacgccgc tctatgccgc gtttactccc agcgaccagt
     4321 atcacgagcg cgctcagcgc gatctactcc agcttgagca tcatcgtctc cccgtgggtg
     4381 tgcttaccac cattctggaa gcgcacacgc ttgtatagtt ccgccccggg gcggcatccg
     4441 cacgtatctg gttcgacgcc atcgcctcga tgacaacgcc aactcatccc acgatggacg
     4501 acttcaaaca ggcgccccag cacatttctt gatttcccga ccagagattg acgatcttcg
     4561 acgcgcacat gtatgtcgtt agcgagcgat tgaacgttcc catctggagt tatgatcacc
     4621 acttgacgtg cttcaggcca agcgttggta ttcgtagagt tgtcgacgtg cagttgcgtc
     4681 ggacgacgtt tgatgatcga tggaggtctt gtgatggttg ctgacagcgt cgatcacgtt
     4741 gcgagtgagc tgcggagtgc cgtttctgcc gaacggttgc aggagcacct gaacgcgttt
     4801 tcgaccctgt tccgcgattc ggggagcgaa gacgagtgga aagcagcccg ctacatccgc
     4861 gaccggatga cggagtacgg cgttcgggcc gagatcctgg agttcgattc actgatctcc
     4921 tggccgcgcg aaggtacgct gaccgttctc gacgccgacg ggaccgccgg cgaggagatt
     4981 cccgttcgga cgcgctcgtt tgggacgacg acaccggccg gcgggatcga ggcggagctg
     5041 gtcttcgtcc cgttcgagca gccggaggcg ggcgcgatga tcttctcgca ccgctcggtc
     5101 gccggtgact acagcgggct ggacgtgcgc ggcaagatcg tgctgacgat ggacggcggg
     5161 ccggacggca tccgccgggc gcaggagcgc ggcgcggccg gccacattca catctggccg
     5221 tcggacgagg ctgtcatcca cgagatgatc ggcaccagcg tctggggaac gccgacgccg
     5281 gaatcggcgc tgcggctgcc ggccatcccc gtcctcggtg tcacgctcac cgacggcgag
     5341 cggctggcgg agcactgtcg ggccgagggc gtccgggtgc ggatgacgtc cgatgtcctg
     5401 acgcaatgga tgcgagtgcc gctggtgatc gccgatgtcg aggccggggc ggcaatcgac
     5461 gaccgcttcc tgctggtcgg cgcccatatc gactcgtggt acgaaggcgt caccgacaac
     5521 gcgaccggcg acgcagcgct gatcgagatg gcgcgggtgt tgcacgagca gcgcagcggc
     5581 ctgcggcatg gcgtgcgctt cggctggtgg ccgggacact cgaccgggcg ctactccggc
     5641 tcgacctggt acgccgacac gcgcttcatc gagctacgcg aacgctgcct gggctacctc
     5701 aacatcgact cgcccggcgt ccgtgagacg gaggtctggg actgccgcta caacatgggc
     5761 gagatcgagc acatcacgtc atccgtcgtc cgcgagctct ccggccagga gccaaacatc
     5821 cgtcgcccgc tgaaggccgg cgaccagtcg ttcctcggcg ttggcctccc gagcatgggc
     5881 gccttccgga tgctgccgca ggaccacccg gatcgcaagg cggtcggcgg ctgtgggggc
     5941 gcctactggt ggcactcgcc ggaggacacg ctcgacaagg ccgacgcgaa catcctcgcc
     6001 gaggacaccc gcatctacgt gacgatggcg gccgagatgt gcgtgccgga agcgcacccg
     6061 tataacttcg tcccgagcgc gaacgacttc ctgacgaacc tgaccgcatt gcaggaggtc
     6121 gccggccagt accttgacct gacaccggcg atcgaggctg cccaacgcta ccgcgccgct
     6181 gcggaacggc tcgccaacca aggtgctggc tcggacgtgg cgcgttacaa cgcaaccgtc
     6241 ctgcgcgtca cgcggatcgt caacccggcc ctcttcacga tcgacggtcc ctacgagatg
     6301 gacccggccc tccagctacc ggtcctgccg ggcctcgcgt caatgcggga gcttgctacg
     6361 ctcgaccccg cttccagcga acaccgcttc ctgctgacga agctcacccg ccagcgcaac
     6421 cggattcagg acgcgttgga tcaggcggtg agggagttgg gtggatgaag cggcgggatt
     6481 cgggacacgg gattcgggat tcgggatttg gggtaagcgt gcgtgcgctg gcccgctgga
     6541 gccgaagcgt tccccagccc cgaatcccgc cgcttgacga cagccccatc agcacgcacg
     6601 atacgcacaa cacattcaac acccagcact cagcactcag cacacaaagg gggtagcggc
     6661 atggtgagcg ggtctgtcgg gggacatctt gatgagattc cgttgttcgc cgagctgccg
     6721 ccggggatgc tggcggtgct ggcgtcggag agccgcgtgc gccgctatcc cgaggggcag
     6781 gtgctgttct ccgagggcga tcccggcgac gcgctgctga ttctcgaaga gggacgtgtc
     6841 aaggtctgcc gcttcatggc cggcgggcag gagatcgtgc tggccgtcca ggaggcgccg
     6901 gcctccttcg gggaactggc gctggtcgat ggcgctcccc gttcggcgac ggtcattgcc
     6961 cagaccgccg tagtcgtccg aatgctgccc cgcgccgcgc tgatggggat gattcacaac
     7021 gagccgaccg tcgcgatggc gctgatggag gggctggtcg ggatgatccg caacaccaac
     7081 gaccggctct cagatctcca ggccctggac gtgccggggc gggtcgcgaa gtggctgctg
     7141 ggctgggccg agcggagtgg ccagcagcgc gccgatggtg tcgcgatccc gttcgagatc
     7201 agccagagcg acctggcggc cgagctcggc acgacccgcg tcagcatcaa caaggcgcta
     7261 cggatgtttg agagcctcga cgtgattgcc tccgacaagg atgagatcgt gatcatgaag
     7321 cctgagcggc tgcaggagta tgtgtattga gtgacgcgtg acgcgtgacg cgtgacgcgt
     7381 cctgatgggg tttggagtgg acagcgggcc tgttgaactg aacgggacgc ggctctacta
     7441 cgaggtctcc ggcccgccgg atgcgccggc cgtcgtcctc gtccacgggt tctcgctgga
     7501 cacgcgcatg tgggccggcc aggtcgggcc gctgtccgag cgctatcgcg tcgtccgtta
     7561 cgacctgcgc ggcttcgggc gctctgccat accggaggcc ggcgtccact accagcatca
     7621 cgacgacctg cgcgcgctgc tggaccagtt ggggatcgag cgagccaccg tcgtcggcct
     7681 ctcgctgggt ggcgccgtca cgctcgattt cgcgctgcat catccacagc gcctgaccgg
     7741 cctggtgctg gccgacgccg tcgtgccggg cttcgacgcg ccggatctcg ccgatctgag
     7801 ccgccaggtc t
//
LOCUS       DASUGJ010000036         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1394399, whole genome shotgun sequence.
ACCESSION   DASUGJ010000036 DASUGJ010000000
VERSION     DASUGJ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394399"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFV93_00955"
     CDS             <1..277
                     /locus_tag="VFV93_00955"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX5163934.1"
                     /translation="GTQWAVFENNGDRLWANIRLTVEDFLFNEWRNGALLGLRPEDAF
                     FVRCDRSTMTQNDLDNGRLVCQIGVAVVKPAEFVIFRIGQFTATSRQ"
     gene            311..769
                     /locus_tag="VFV93_00960"
     CDS             311..769
                     /locus_tag="VFV93_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX5163935.1"
                     /translation="MAVQRDNPYGAFNFRVTVDKFGDADSVQAGFQEISGLGLEVTVA
                     EYRNGNEKENHVRKMSGIYKASDVTLKRGVIGYTDFFDWIKEVRQGDQAVRRTVKIDL
                     MNEENTSEVMTWTLTNAKPMKYTGATLNAKGGTDVAIEELVLSCEKLDVA"
     gene            775..>2889
                     /locus_tag="VFV93_00965"
     CDS             775..>2889
                     /locus_tag="VFV93_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX5163936.1"
                     /translation="MSVVSDRRVPGVYFLPPPVRRGLGLPPLDVAAFVGFAEKGPVDL
                     PVPIGDVNTYREIFGGDLALAREDGERTVYANLPQSVAAFFANGGRRCYVVRVAGVGA
                     TNTRCRLPGLVALGRDGIQLASIRASSPGRWSAGFRLATRLDRVPLPLDAFTHAPETP
                     RTLRWDARAAPAAIQTGDLLRLTFVDEAGNRQQVLVPVSSVTRGADAAEQVTLSTSQI
                     YSVVPPAAIQPSWTVADSRRLTTDASADDVFVFDLRIDDDAILLTADMDDEKALGEGD
                     VLLLTISLDPAHAGTFLFPVTGVRQAGGNSSPPDSSPPEGSVELRATTMLRLDADQTV
                     TGTLAGVERLRFELALRDGDQRRATIGDLAFNAGHPTYWGEVALLDSSPLRPRPAVVG
                     ANVVQSVLETPEAEAARIYNLLQTDQRIDPVRDGVPSPAMLAGLLAPVSGDGLLFLPL
                     DMPGVLTDDRFVAPSTSDVGRDGLDRFDGGSFNWFVDERLTLTPSDPSLRTAELMGNA
                     FDIQYVQNKRLRGLHSLLWIDEVALISVPEAVQRNWSPAAPPPGDVVLPAREPAAPEP
                     CPPSGPFLYCIRPPEIAEIEPATGPLRGGTQVVVRGEGFDPLGATTVSFDGVPASDVV
                     VLSGIELHCTTPTAGVARPVVVDVTTSAGTGTLPSGFRYLPETVEPVLPELAPIGDYD
                     IDAATSPLLPLQRELLRCCVARG"
BASE COUNT          460 a          933 c         1014 g          482 t
ORIGIN      
        1 ggggacgcag tgggccgtct tcgagaacaa cggcgaccgg ctctgggcca acatccggct
       61 gacggtcgag gacttcctct tcaacgagtg gcgtaacggc gccctgctgg ggctgcggcc
      121 ggaggacgcc ttcttcgtcc gctgcgaccg ctcgacgatg acccagaacg acctcgacaa
      181 cgggcggctg gtctgtcaga tcggtgtcgc tgtcgtcaag ccggctgagt ttgtcatctt
      241 ccggatcggg cagttcacgg ccaccagccg tcagtagcgc cagcgcagca ccaggaagtg
      301 aggcatcccg atggcagtcc agcgagacaa cccgtatggc gcgttcaact tccgcgtcac
      361 cgtcgacaag ttcggcgacg ccgactcggt ccaggccggc tttcaggaga tcagcgggct
      421 cgggctggag gtgacggttg ccgagtaccg caacggcaac gagaaggaga accacgtccg
      481 caagatgagc ggcatctaca aggccagcga cgtgacgctc aagcgcggcg tgatcggcta
      541 caccgacttc tttgactgga tcaaggaggt gcgtcagggc gatcaagccg tccgcaggac
      601 ggtcaagatc gacctgatga acgaggagaa cacgtccgaa gtcatgacct ggacgctgac
      661 caacgccaag ccgatgaaat acaccggagc aacgctgaac gccaagggag gcaccgacgt
      721 cgcgatcgaa gagctggtcc tctcgtgcga aaagcttgac gtggcgtaag tccgatgagt
      781 gtcgtcagcg atcgccgcgt gccgggtgtc tacttcctgc cgccgccagt ccggcggggt
      841 ctcggcctgc cgccgctgga cgtagccgcc ttcgtcggct tcgccgagaa gggtccggtc
      901 gatctgccgg tcccgatcgg ggatgtcaac acgtaccggg agatcttcgg cggcgatctg
      961 gcgctggccc gcgaggacgg cgagcggacg gtctacgcca acctgccgca gtcggtcgcg
     1021 gccttcttcg ccaacggcgg acggcgctgc tacgtcgtgc gggtagccgg ggtgggcgcc
     1081 acgaacacac gttgcaggtt gccgggactg gtcgcgctcg ggcgggatgg catccagctc
     1141 gcgtcgatcc gcgcttcgtc gccggggcgc tggtcggccg gcttccggct cgcgacgcgg
     1201 ctggatcggg tgccgctgcc actggatgcc ttcacccacg cgccggagac gccgcgaacg
     1261 ctgcgctggg acgcgcgggc agcgccggcc gcgatccaga ccggcgacct gctgcggctg
     1321 acgttcgttg atgaggcagg caaccggcaa caggtgctcg ttcccgtctc gtcggtgacg
     1381 cggggcgccg acgccgccga acaggtgacg ctctcgacca gccagatcta cagcgtcgtg
     1441 ccgcctgctg ccattcagcc aagctggacg gtcgccgatt cgcgccggtt gacgactgac
     1501 gcatcagcgg acgatgtgtt cgtcttcgat ttgcggatcg acgacgacgc gatcctgctg
     1561 acggccgaca tggacgacga gaaggcgctc ggtgagggcg atgtccttct gctgacgatc
     1621 agcctcgacc ccgcgcacgc tgggacgttc ctgttcccag tcaccggcgt tcgtcaggcc
     1681 ggaggcaaca gttcgccgcc agatagctca ccgccggaag gctcggtcga gctgcgtgcg
     1741 acaacgatgc tgcggctcga cgcggaccag actgtcaccg gcacgctggc cggggtcgag
     1801 cggttgcgct tcgagctggc gctgcgcgat ggcgatcagc ggcgggcgac aatcggcgat
     1861 ctggccttca acgcggggca tccgacctac tggggcgagg ttgcgctgct ggattcgagc
     1921 ccgctacgcc cgcgaccggc ggtggttggc gcaaatgtcg tccagagcgt tctggagaca
     1981 ccggaagccg aggcggcgcg gatttacaac ctgttgcaga ccgaccagcg catcgacccg
     2041 gttcgtgatg gtgtcccgag tccggcgatg ctggccggtt tgctcgcgcc ggtcagtggc
     2101 gacggcctgc tcttcctgcc gctcgacatg ccgggcgtcc tgactgacga tcgcttcgtt
     2161 gctccatcca cgagcgacgt tggccgtgac gggctggacc ggtttgatgg cggctcgttc
     2221 aactggttcg tcgatgagcg cctgacgctg acgcctagcg acccgtcgct gcggacggcc
     2281 gagctgatgg gcaacgcgtt cgacatccag tacgtccaga acaagcggct gcgcgggctg
     2341 cacagcctgc tctggatcga cgaggtcgcg ctgatcagcg ttccggaggc ggtgcagcgc
     2401 aactggtcgc cggccgcgcc gccgcccggc gatgtggtcc tgccggctcg agaaccggca
     2461 gcgccggagc cgtgtccgcc atcagggccg ttcctgtact gcatccgccc gccagagatc
     2521 gccgagatcg agcctgccac cggtccgctg cggggcggga cacaggtagt cgtccgtggc
     2581 gaggggtttg acccgcttgg agcgacgacg gtcagcttcg acggcgttcc ggcgagcgat
     2641 gttgtcgtgc tgtcggggat cgagttgcat tgcacgacac cgacagcggg cgtcgctagg
     2701 ccggtggtag ttgacgtaac gaccagcgcc gggaccggca cgctgccgag cgggttccgc
     2761 tacctgccgg agacggttga gccggtcctg ccggagcttg cgccgatcgg cgattacgac
     2821 atcgacgcgg cgaccagccc gttgctgccg ctgcagcgcg agctgttgcg ctgctgcgtt
     2881 gcgcgtggc
//
LOCUS       DASUGJ010000037         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_282919, whole genome shotgun sequence.
ACCESSION   DASUGJ010000037 DASUGJ010000000
VERSION     DASUGJ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282919"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2240)
                     /locus_tag="VFV93_00970"
     CDS             complement(<1..2240)
                     /locus_tag="VFV93_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5163937.1"
                     /translation="MASQQRVFISYHRSDEVIARQVREQLASHGVASWMDQYDIPVGA
                     YWPDKIDKGLASCGTVIGILSPASVESRNVKNEWDWTIQNGKLLLLVQVSPCVIPHRY
                     VSINYIDGTSGDEQQVIASLFTALGVSPQIAPVTAPDGAGPPVRSNQRRAGPRLLKPL
                     IVGRDTELKLLQQTLDDLRVARGSLVLVSGEAGIGKTTLTTWLLAEAEERGLLPLSGG
                     CYDLTTTPPYGPWAEILAGFPEDDDLHQVPAQLRAGGGLAGIDSQAALFDLTIRFLTS
                     VAEVRPLVLLLEDLHWADPASLDFLRFASRSIQHTSILLLATYRDDEITRDHPLSALL
                     PALVREAPVHRLHLGRLERPAVLDLVRERYELPAGDEARLIAYLLDLAEGNPFFTREL
                     LHSLEEQRLLVLSVGAWALADLADSSVPSLIHQIIEGRLARLDLATRSLLERAAVVGF
                     DTPLDILQTLDGTDDRELDDALHRAIEHHIVTINHGSRSIHFSHALVRQAIYQAIPPL
                     RRQALHRAVGGLLAERPRADRSIVANHFFEARDERALDWLVRSARAAQRLFAPETVIA
                     ECSRAITLSDALGRDISPSAFRLRSWARDSTGDFDGALHDLTEALKQARNADDQPEEW
                     QSLLDLAELWAARDYQRTGEYCRQAVEIARTMGDQAALGHSLNRLGNWHANADLPSAA
                     LRYHDEAQQIFEAIGDQHGLASTLDLLGTTYAVIGDVTQEMRNYELAISLLRQLDDRR
                     TLSSALA"
BASE COUNT          428 a          707 c          808 g          429 t
ORIGIN      
        1 gcgagtgcgg agctgagggt gcgccgatcg tcgagttgac gcaggagcga gatcgccagt
       61 tcatagttgc gcatctcctg tgtcacgtca ccgatcaccg cgtaggtcgt cccgaggaga
      121 tcgagggtgc tggccagtcc atgttggtcg ccgatcgcct cgaagatctg ctgcgcttcg
      181 tcgtgatatc ggagcgccgc tgacggtagg tcggcgtttg cgtgccagtt gccgaggcgg
      241 ttgaggctgt gacccagcgc ggcctgatcg cccatcgtcc gggcgatctc gactgcctgg
      301 cggcagtact cgccggttcg ctggtagtcg cgcgctgccc agagttcggc cagatcaagg
      361 agcgactgcc attcctcggg ctggtcgtcg gcgttgcgag cctgtttcag tgcctcagtg
      421 aggtcgtgca gtgctccgtc gaagtcgccg gtagagtcac gcgcccaact ccgtagccgg
      481 aatgcgctcg gcgagatgtc tcgaccaaga gcgtcagaga gcgtgatggc ccggctgcac
      541 tcggcgatga cggtctctgg cgcgaagagc cgctgggccg ccctggctga ccgaaccagc
      601 caatccagcg cacgttcgtc tctggcctcg aagaagtggt ttgcaacgat cgaccgatcg
      661 gcgcgtggcc gctccgcaag cagcccaccg acagcgcggt gcagcgcctg ccggcgcagc
      721 ggcgggattg cctggtagat cgcctggcgc acgagcgcgt gactgaagtg aatcgatcga
      781 ctgccgtggt tgatcgtgac gatatggtgc tcgatcgctc gatgcaatgc gtcatccagt
      841 tcgcggtcgt cggttccatc cagtgtctgg agaatgtcga gcggtgtatc gaagccgacg
      901 acggcagccc gctcgaggag tgagcgtgtc gcgaggtcga ggcgggccag gcgtccttcg
      961 atgatctggt ggatcagcga cggcacgctc gaatccgcca ggtcagccaa cgcccaagcg
     1021 ccaactgaga ggacgagcag tcgctgttct tcgagactgt ggagcagctc cctggtaaag
     1081 aacgggtttc cttcagccag atcgagcagg tacgcgatca ggcgcgcttc gtcgccggcc
     1141 ggcagttcgt agcgctcacg aaccaggtcc agcacggccg gccgttcgag ccgcccgaga
     1201 tgcaggcggt ggactggagc ttcgcgcacc agcgccggga gcagtgccga aagcggatga
     1261 tcgcgggtga tttcgtcgtc acggtaggtt gcgagcaaca gaatcgacgt gtgctggatc
     1321 gacctggagg cgaagcgcag gaagtcgagg ctggcgggat cggcccaatg cagatcctcc
     1381 agcagcagca ccagcggacg tacttccgcg accgatgtga gaaatcgaat ggtgagatcg
     1441 aagagcgccg cctgtgaatc gatgcctgcc agaccgccac ccgcacggag ttgtgcggga
     1501 acctgatgga ggtcgtcgtc ttccgggaaa ccggcgagga tctcggccca gggaccgtag
     1561 ggaggggtcg ttgtcaggtc gtagcaaccg ccggagagcg gcagaaggcc gcgctcctca
     1621 gcctcagcca gcagccaggt cgtcagcgtc gttttgccga tgccggcctc gccactgacg
     1681 aggacgagcg aaccccgcgc gacgcgcagg tcatccagtg tctgttgcag tagcttcaac
     1741 tcagtgtcgc gcccgacgat cagcggtttg agcaggcgcg gcccggctcg gcgctggttg
     1801 gagcgtaccg gcggaccggc gccatccggg gctgtcaccg gtgcgatctg tggtgataca
     1861 ccgagtgcgg tgaacaacga ggcgatcacc tgctgctcgt caccacttgt cccgtcgatg
     1921 tagttgatcg agacgtatcg gtgaggaatg acgcatggcg agacctggac gagcagcagc
     1981 agcttcccgt tctgaatcgt ccagtcccac tcgttcttga cgttgcgact ctcgaccgac
     2041 gccggtgaca ggatcccgat gactgtcccg caactggcca gccctttgtc gatcttgtcc
     2101 ggccagtagg cgccaaccgg gatgtcgtac tggtccatcc agcttgcgac gccgtgggat
     2161 gcgagttgct cgcggacctg gcgggcaatc acctcgtcgg aacgatggta ggagataaag
     2221 acccgttgct gcgatgccaa cgttgtccgc cagtccgtac gagatgcgac ggaatgcgag
     2281 atgtgttccg gtggagaacg gtcatacgga tcgtataccg acagcataac gaggctgaac
     2341 cgctcaggca agcggtccag ccgggacgaa tt
//
LOCUS       DASUGJ010000038         3568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1811333, whole genome shotgun sequence.
ACCESSION   DASUGJ010000038 DASUGJ010000000
VERSION     DASUGJ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811333"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VFV93_00975"
     CDS             complement(<1..775)
                     /locus_tag="VFV93_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HEX5163938.1"
                     /translation="MVANATTTHPDTMNAASRLDPLRILAVDVGAGTQDILVYESDRP
                     IENCVKLILPSQTQVVGGRIRRATAAGRPIYLTGAVMGGGASSAAVEAHLAAGLPVYS
                     APEAARSLHNDLERVKQMGVVLRDVPPPGAEIIWLRDIDLAALADTLGQFGVEIPSLY
                     AIAVQDHGYLPGSGGREFRYEFLQSLLEGGGTVERMAYHDPPEYMIRMRSVIESLPGA
                     VVMDTGAAAVAGVLGDPVVSQAADGDGAILVNIGNMHTFG"
     gene            complement(839..1786)
                     /locus_tag="VFV93_00980"
     CDS             complement(839..1786)
                     /locus_tag="VFV93_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5163939.1"
                     /translation="MHRRGIVAGLLGVVTLATGVLAYMRRIAPTWLEVVRLRVALAGL
                     PDEWVGVRIAHLSDFHAGGAIPLDFLQRAKAIALADRPDVVALTGDFYDEGVPSDAGD
                     LFTAWPDGLLVVAVLGNHDYRATEDDLAALKRELYTSGVQLLDNRAIKIDLRGRSAWI
                     VGVDDPYSWKMDEAQAFGSLPATEDALIYLAHSPVAAQTLPVGRARLMLAGHTHGGQV
                     RLFPDGRVPFVSFLRRLQGNPQRPDPDIHRGIHWRRGAVLVISNGLGLSKLPWRFRTR
                     PQVILIELVRAPATGPACDDAARYVQRLNPTPFWQRWLS"
     gene            1940..2635
                     /locus_tag="VFV93_00985"
     CDS             1940..2635
                     /locus_tag="VFV93_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX5163940.1"
                     /translation="MVVITAAMEDYLKVIYRLSEDGKRATTQAIAERMNVAAPSVTGM
                     IKRLADLKLVEHERYRSVSLTAAGQKAALEIVRHHRLLELYLAEALGYSWDQVHDEAE
                     RLEHTISEEFEARIDRALGYPTTDPHGDPIPTAAGAIQSVPDDRLSALEPGERATISR
                     VDDTSPDKLRYLGTMGLYPDTEVSVIERQPFGGPLLIKVGAAEHYVGAELADTVHVDR
                     TPVPPIHDESAEG"
     gene            2663..>3568
                     /locus_tag="VFV93_00990"
     CDS             2663..>3568
                     /locus_tag="VFV93_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX5163941.1"
                     /translation="MVTHTRAHAPNVWVEPPPLSAADLAITDAPLLSAILHARGVRSA
                     DAAARFLSPTTIPLNDPLLMPDMERAVARIKAAIAAGRRIVVFGDYDVDGLTSTAMIV
                     RVLRRLGGQPSAVIPHRMDDGYGLSPTVVERLLAAGAELLVTVDCGSSNRAEFEALLQ
                     HGVEIVVVDHHRHAGELPAEVAYVSPRRPDNQYPFTDHAAVGVCYTLVRALLGDDDAE
                     MYLPYVALGTIADVVDLRDENRALVTRGLGMLRRWKLPGLKAICASAGIDQQAVGAFE
                     IGYLIGPRLNAAGRVDSPQAALDLLL"
BASE COUNT          622 a         1243 c         1146 g          557 t
ORIGIN      
        1 cgccgaaggt gtgcatgtta ccgatgttga cgagaatggc accatcaccg tccgccgcct
       61 gactgacgac cgggtcgccg aggacgccgg caaccgccgc cgcgccggtg tccatgacga
      121 ccgcgccggg caggctttcg atgacgctgc gcatccggat catgtattcg ggcgggtcgt
      181 gataggccat ccgttcaacg gtaccgccac cttccagcag cgactgcagg aactcgtagc
      241 ggaactcgcg cccgccggag ccgggcaggt agccgtggtc ctgaacggcg atcgcgtaga
      301 gcgacggaat ctcgaccccg aactgcccca gcgtgtccgc cagcgcagcc agatcgatgt
      361 cacgtagcca gatgatctcc gcccccggcg gcggcacgtc gcgcaggacg acacccatct
      421 gctttactcg ctcgagatcg ttgtgcagtg agcgggctgc ctccggcgcc gagtagaccg
      481 gcaagccggc cgcgagatgc gcctcgaccg ctgccgagga cgcgccgccg cccatcaccg
      541 cgccagtcag gtagatcgga cggcccgccg ccgtcgcccg gcggatgcgg ccgccaacga
      601 cctgcgtctg cgacggcagg atcagcttga cgcagttctc gatcgggcga tcactctcgt
      661 agacgaggat atcctgcgtc ccggccccga catcgaccgc cagtatccgc agcggatcga
      721 gccggctggc ggcgttcatc gtgtccggat gagttgtcgt tgcattggct accatgccct
      781 cgactgtgct tcatctccac caaaccgtca acccgaccgt tcggtcaggt catgccggtc
      841 aggacaacca gcgctgccag aacggggtcg ggttcagacg ctggacgtag cgagcggcat
      901 cgtcgcacgc cggtccggtg gcgggagcac ggacgagctc gatcaggatc acctgtgggc
      961 gcgtccgaaa gcgccagggt agcttcgaga gaccgagccc gttggagatc accaggaccg
     1021 cgccacgccg ccagtggatc ccgcgatgaa tgtcgggatc gggtcgctgc ggatttccct
     1081 gcagtcgtcg caggaacgaa acgaacggca cccgaccgtc tggaaagagt cgcacctgcc
     1141 cgccgtgggt atggccggcg agcatcagcc gcgcccggcc aaccggcagt gtctgcgcgg
     1201 cgaccggcga gtgggccagg tagatcaacg cgtcctccgt cgccggcagc gaaccgaacg
     1261 cctgcgcctc gtccatcttc cacgagtacg gatcgtcgac gccgacgatc caggctgatc
     1321 ggccgcgcag atcgatcttt atcgcccgat tgtcaagcag ttggacacca gacgtgtaca
     1381 gttcgcgctt cagcgctgcc agatcatcct cggtcgcgcg gtagtcgtga ttgccgagga
     1441 cggcgaccac cagcagacca tccggccagg ccgtgaagag gtcacccgcg tcactcggca
     1501 cgccctcatc gtagaagtca cccgtcaacg cgacgacgtc gggccggtcg gccagcgcga
     1561 ttgccttggc gcgttgtaga aagtcgagcg gaatcgcgcc gcctgcatga aagtccgaca
     1621 ggtgcgcgat gcgcaccccc acccactcgt cgggtagacc agccaacgcc acgcgcagac
     1681 ggacgacctc cagccaggtc ggagcgattc ggcgcatgta ggccagtacg ccggtcgcca
     1741 gcgtcacgac cccgagcagg ccagctacaa ttccacgtcg atgcatcgac tccctatcag
     1801 catggcccgt ggcctgttcg tcggacaccc tgtgcgactg ccgtaacggc gcgccagacg
     1861 cgcgacttgc cacggatcgc gtccagagga taccgttaga cacagcatct gtccgttcac
     1921 cctcgatgga gcgtgtgccg tggttgtgat caccgccgcg atggaagact atctcaaggt
     1981 gatctaccgc ctctcggagg acggcaagcg cgcgacgact caggccatcg ccgagcgcat
     2041 gaacgtagct gctccgtcgg tcaccggcat gatcaagcgg cttgcggacc tgaagctggt
     2101 cgagcacgag cgctatcgct cggtctcatt gacggcggcc ggacagaagg ccgcgctgga
     2161 gatcgtgcgc caccaccggc tgctggagct ctatctggcc gaggcgctgg gctactcgtg
     2221 ggaccaggtc cacgacgagg ccgagcggct cgagcacacg atctccgagg agttcgaggc
     2281 gcgcattgac cgggcgctgg gctacccgac gaccgacccg cacggcgacc cgatcccgac
     2341 cgccgccggc gcgattcagt cggttccgga cgatcgcctc tcagccctgg agccgggcga
     2401 gcgggcgacg atcagccgcg tcgacgacac cagccccgac aagctgcgct acctcggtac
     2461 gatggggctc tatccggaca ccgaggtgag cgtgatcgag cgccagccat ttggcgggcc
     2521 actgctgatc aaggtcggcg cagccgagca ctacgtcggc gcagagctgg ccgacacggt
     2581 ccacgtcgat cgaacacccg tccctccgat ccacgatgaa tcggctgaag gatagcgacc
     2641 cgcgaaagga agcagacagg cattggtcac ccacacacgc gctcatgccc cgaacgtctg
     2701 ggtcgagccg ccgccgctgt ctgctgccga tctcgccatc accgatgcac cactgctgag
     2761 cgccatcctc cacgcgcgcg gggttcgatc cgctgacgca gcggcccggt ttctgtcgcc
     2821 aacaacgatc ccgctcaatg acccgctgct gatgcctgac atggaacggg cggtcgcccg
     2881 gatcaaagcc gcgatcgccg ctggtcgcag gatcgtcgtc ttcggcgatt atgacgtcga
     2941 tggactgacc tcgacggcga tgatcgtgcg cgtcctccgc cggctcggcg gccagccatc
     3001 ggccgtcatc ccccaccgga tggacgatgg ctacggcctc agccccacgg tcgtcgagcg
     3061 gctgctggcg gccggggccg agctgctagt caccgtcgac tgcggcagca gcaaccgcgc
     3121 ggagttcgag gctcttcttc agcacggcgt cgagatcgtc gtcgtcgatc atcaccggca
     3181 cgcgggcgag ctgccggccg aagtcgccta cgtctcgcct cgccgtccgg acaaccaata
     3241 cccattcacg gaccacgctg ccgtcggtgt ctgctatacg ctcgttcgcg cgctgctggg
     3301 cgacgacgac gcggagatgt acctgccata cgtcgcgctc gggacgatcg ccgatgtcgt
     3361 cgatctgcgc gatgagaacc gcgcgctggt gacacgcggg ctgggcatgc tacgtcgctg
     3421 gaagctgccc ggcctcaagg cgatctgcgc atcagccggc atcgaccagc aggcggttgg
     3481 cgcgttcgag atcggctacc tcatcggccc gcgcctgaac gccgccggcc gggtcgactc
     3541 gccccaggcg gcgctcgacc tgttgctg
//
LOCUS       DASUGJ010000039         8733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1255396, whole genome shotgun sequence.
ACCESSION   DASUGJ010000039 DASUGJ010000000
VERSION     DASUGJ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255396"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VFV93_00995"
     CDS             complement(<1..1145)
                     /locus_tag="VFV93_00995"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5163942.1"
                     /translation="MTPALHRHALTRQNVAWPVAIALALGLALVAVFVAPIMLAVVPM
                     LVVAGLALLRWPWLGVALLVASVPAQQAGAVSGLTLTRAALVATGAGLVIWWTAGRRP
                     IVATRLIWPFVALIAWMLVTTFVARDLGAASADLFRWSVALFAFFAALQVLIKTSERL
                     LTAIIVAVAIAGAIEAGVGAALGLLGFGPESFVIDDAFARAYGTFGRPNTFAGYLEMA
                     FFPVLWLGLYRLRDLNDRLRGYIVARRAGFTASAAQRRDLLLATGVTTILLGSAAVML
                     AGIAISFSRGGWLGLSAGLVVTVLIAVRRYWRLVLPAIPVVMLLGLTALAAFAPATLT
                     ERVSSISEEARPFDAASIPITPENFAVVERMAHWQAGWHMFEDHPVTG"
     gene            complement(1142..2083)
                     /locus_tag="VFV93_01000"
     CDS             complement(1142..2083)
                     /locus_tag="VFV93_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX5163943.1"
                     /translation="MLVSGGAGFIGSHLCDSLIADGHQVVCVDNFITGRRKNIEHLLG
                     NASFELIEHDIVNELDDSIAVDRVFHLASPASPVGYMRYPIETHLVNSIGTHNMLRLA
                     KRCGATLLYTSTSESYGNPDVHPQPETYFGNVNPVGPRSCYDESKRYGESITMEYVRQ
                     FGLDARIIRIFNTYGPRNDPNDGRVIPNFIRSAERGEPLIIFGDGSQTRSLCFVSDLV
                     RGLRLALESPSTMGQVINLGNPDERTVADLARIITEYCQSSATIEFVDARQDDPERRC
                     PDITKARALLGWEPTIGLEEGLRTTIEHFRVAETLHT"
     gene            complement(2034..3470)
                     /locus_tag="VFV93_01005"
     CDS             complement(2034..3470)
                     /locus_tag="VFV93_01005"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5163944.1"
                     /translation="MSSLATADARPALAVCVASCLAFALAGLTGFSPLALLALIVAVA
                     LACFSPVGAIAAAVASLPYFYRPLDIAGQHVAASELLLFAALLGTGLQVLVAAVESPA
                     GALRKVQRRLLPVLRSPLAIAAVGLVGLGGLLVLMPYDPTHRADSLREWRWTLFEPAL
                     LLGLLIWHRRDPILRELVAIALIAGGTVASLHALGDYALGGGVAVGGVTRIAGPYPHP
                     NALALMTVRVAALAFVWAVVDPRVRRWIIPLLVICAVTVAATFSRGAMLAGLVAVAIV
                     LVNAPPRIRLAGAGSVIAGLALAVAVARDRMLDLFGGGSGSLRIDIWTSALRMIDDRP
                     VRGYGPDQFLYAYLPRYVQPTAWGERFTAHAHNFLLDFWIRLGIIGAAFVGFVVLMCL
                     LALARRLRSPIRSQPLGMAALVALVAIIANGLVDNAFFSHDLAMSCWLLAWLAFGAES
                     DGAGAEGGVEIARAGVGRGWLYRLTPLR"
     gene            complement(3448..4827)
                     /locus_tag="VFV93_01010"
     CDS             complement(3448..4827)
                     /locus_tag="VFV93_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163945.1"
                     /translation="MIPSFDPALWRRASWTVLTLAVAAFVCLGLAEQGSRGGGLLIPP
                     DSPLPFERQRVFGLNLDALSSVDALNWLNAAGNPNLALMVLPVDADVVQSLALEDQRE
                     NGLAGIETLRAAAGGSPLAFCLRRPAETVGGLAIAQAAVESIKARFPDQVIYVRACDP
                     DAVPGWQGNIDEAARPDAEVRASDDTLIPLAGGPLITLDAIDNVRELDPDRLSLNAHD
                     RYTIYSFEASEPLSTDDVERVAGALADTSHTALVFVAPIDGVDPAALISTIAPVALAG
                     DELPEGFSGVKAPAMQINDGWQHSTVGTTQYLRAAASGAALEVEFVGTDISLIAVESP
                     DSGLINVWLDPASQTAPPTAILDLSSTQARDSAIPVATGLPAAHHRLIMQVVTEEGQS
                     VTISGLFVTGKPPTAWTVTMAAAVVLLAAVAALSERCYTAIAAIRRRSGPPRRRSRTG
                     HPRVFARDR"
     gene            4960..5844
                     /gene="mtnP"
                     /locus_tag="VFV93_01015"
     CDS             4960..5844
                     /gene="mtnP"
                     /locus_tag="VFV93_01015"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX5163946.1"
                     /translation="MRQRQPALGIIGGSGLYGLSSLTNVETVEVETPWGTPSEAIRIG
                     EIAGQTVAFLSRHGAGHRISPTEIPYAANVCAMKMLGVSRLVSISAVGSLREDCPPRS
                     FVVPDGTIDRTLTRQRTFFDNGIVAHVSIADPFCPTLSALLASVAETGGLPVHRGGTY
                     IAIEGPQFSTRAESALFRSWGASVIGMTAMPEARLAREAELCYSCLAMVTDYDVWHEE
                     DGPVTVDAVLANLFAMTDAVQTIVTTLAGEALPDCDAGCSDALSNAIATSPDAIDAAT
                     RARLAPIAGRYLDAESAG"
     gene            5844..7145
                     /locus_tag="VFV93_01020"
     CDS             5844..7145
                     /locus_tag="VFV93_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5163947.1"
                     /translation="MLQRIRFTELQLLIVPSAMTIVGLLTIYLASTRDLAWEWRDIWV
                     SLAFMGAVFFISIWLSAIGFQGDQVLFPVVVCISGLGLLVIQRLAPDLNEIDPAAYGN
                     LARNQIIYLTAGLILMLGVCTFVRQIGWLRRYKYTWAFGGIGLMLATMVFGTEYYGAR
                     LWINVGPFSVQPSEIAKVAMVVFLAAYLAENRDLISSNYRIGPFSIPPLPYLMPMLLM
                     WGFSILIVVAQNDLGTALLFFGVFLSMLYLASGRLLYVVTGLVTFAIAVYIAYNMFDR
                     IGVRVAGWLNPWADPLDTGFQPVQSEYAMATGKLFGTGLAQGHPTFITQVHSDYIFAA
                     IGEELGLMGTLVVLLLFLLLVFRGFYIALNARDTFGRLLAAGLTTILALQALIIVGGT
                     IRLIPLTGITLPFISAGGSSLLTNFIIVGLLMRISDPTVTR"
     gene            7145..>8733
                     /locus_tag="VFV93_01025"
     CDS             7145..>8733
                     /locus_tag="VFV93_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5163948.1"
                     /translation="MTTFIRLSAFIGALLVLAYGFGGTEEQADKVWLMLLLAAGILLA
                     IAWWPRGTRTLPVFNRTLLRWSAIILVCFTLVSIQLVRVQIVESSRTTSRIEEAPNGE
                     IASNPRERLRSLEMQRGRIFDREGRVLADTVLRDDGTYQRTWPELSTAPLIGYYSPEM
                     YGSTNLEAAYDEYLSGLEGGNPVEEWLNGILHEQQHGYDLGLTIDLDLQNLAAELMDG
                     RPGAVVLMDADTGEVLTMYGAPSFDPNQLYANLGQQSSEEIDAIENYWAELNADPNAP
                     LVFRPTDGLYNPGSTFKSVTASAVIDSGTSTLETVYRDEGVFEVDGRVIEEANRPDPN
                     KVNWTLEESYAYSLNVVFAQIGLQLGSQELWDYAHKFGFGESIPFDFDTATTQIANSR
                     DDLNNSALLADTGFGQGQILATPLQMAMVVSAVVNDGVMMQPHVVDHIQEQDGDVLET
                     FGADEWRRAISADSAAAVRNLLLATADYGYSQGALIDGLTVGGKTGTAEVGDGEPHSW
                     FTGFAESGDRTLVVSVIVEHGG"
BASE COUNT         1602 a         2768 c         2930 g         1433 t
ORIGIN      
        1 ccggtgacgg gatggtcctc gaacatgtgc cagccggcct gccagtgcgc cattcgctca
       61 acaacggcga agttctccgg cgtgatcggg atcgatgcgg cgtcgaaagg acgcgcctct
      121 tcgctgatgg atgagacgcg ctcggtcaac gtcgccgggg cgaaggccgc cagggccgtc
      181 aggcccagca gcatcacgac gggaatcgcc ggaagcacga gccgccagta acggcggacg
      241 gcgatcaaca ccgtcacaac caggccggcc gacaggccga gccagccacc gcgtgagaag
      301 ctgatggcga taccggccag catgactgct gctgatccaa gcaggatcgt cgtcacgcca
      361 gtcgccagca gcagatcacg gcgctgggca gcgctggccg taaagccggc ccgtcgcgcc
      421 acgatgtagc cgcgtaggcg gtcgttcagg tcgcgcaggc gatagagccc aagccagagg
      481 accgggaaga acgccatctc caggtagccc gcgaacgtgt tcggccggcc gaaggtgcca
      541 taggcgcggg caaacgcgtc atcaatcacg aagctctccg gaccgaagcc cagcagcccg
      601 agcgcggcgc caacgccggc ctcgatcgct cctgcaatcg cgaccgcgac gatgatcgcc
      661 gtcaggaggc gttcagatgt tttgatgagt acctgaaggg cggcgaagaa cgcgaacagc
      721 gcgaccgacc agcgaaacag atcggccgag gccgcgccga ggtcgcgggc gacgaaggtg
      781 gttaccagca tccaggcgat cagggcgacg aagggccaga tcagccgggt agcgacgatc
      841 gggcgccggc cggcggtcca ccagatgacc aggccggcgc cggtcgccac cagcgccgcg
      901 cgggtcagtg tcagaccgct gacggcgccg gcctgctgcg ctgggacgct ggcaaccagc
      961 agcgcgaccc ccagccacgg ccagcgcagc agggccagcc cagcgacgac cagcatagga
     1021 acgactgcca gcatgatcgg cgcgacgaaa acggcgacga gggcgaggcc gagcgccagc
     1081 gcgatggcga cgggccaggc cacgttctga cgggttagcg catgccggtg gagagccggg
     1141 gtcacgtgtg caacgtctcc gcgacgcgaa agtgctcgat cgtcgttcgc agaccttctt
     1201 cgaggccgat cgttggttcc caaccgagca gtgcgcgggc cttcgtgata tcgggacagc
     1261 ggcgctcagg gtcatcctgg cgggcgtcga cgaactcgat cgtcgccgac gattggcagt
     1321 actcagtgat gatccgggcc aggtctgcca ccgtccgctc atcgggattg ccgaggttga
     1381 tgacctgacc catcgtcgac gggctctcga gcgccagccg aagcccgcgc acgagatcgg
     1441 agacgaagca gagcgaacgt gtctggctgc catcgccgaa gatgatcagc ggctcacccc
     1501 gttcggcgga gcgaatgaag ttcggtatga cgcggccatc gtttgggtcg ttgcgaggtc
     1561 cataagtgtt gaaaatacgg atgatgcggg cgtcgagccc gaactggcga acatactcca
     1621 tcgtgatcga ctcgccgtag cgtttgctct cgtcatagca cgagcgtggg ccgactggat
     1681 tgacgttgcc gaagtacgtc tcgggctgtg ggtgaacgtc cgggttgccg tacgactcgc
     1741 tggtcgaggt gtagagcaag gttgcgccgc agcgctttgc gaggcgcagc atgttgtggg
     1801 tgccgatcga gttgacgagg tgcgtctcga tcggatagcg catgtagccg accggactgg
     1861 cggggctggc gaggtggaag acgcggtcga cggcgatcga gtcatcgagt tcgttgacga
     1921 tgtcgtgctc gatcagttcg aacgacgcgt taccgagcag gtgctcgatg ttcttgcggc
     1981 gaccggtgat gaagttgtcg acgcagacga cctggtggcc atcggcgatc aggctatcgc
     2041 agaggtgtga gccgataaag ccagccccgc ccgacaccag cacgcgcaat ttctacccct
     2101 ccttcagcgc cggcaccatc ggattccgct ccgaaagcca gccaggcgag tagccagcag
     2161 ctcatcgcca gatcgtgcga gaaaaacgca ttatcgacca aaccgtttgc gatgatcgcc
     2221 accagggcga ccagcgccgc cattcccagc ggctgggaac ggatcggact gcgcagtcgg
     2281 cgcgccaacg ccagcagaca catgagtacc acaaatccaa cgaaggcggc gccgattata
     2341 cccagcctga tccagaaatc gagcaggaag ttgtgtgcat gggcggtgaa gcgttctccc
     2401 caggcggtcg gctgaacata acgcgggaga taggcgtaga gaaactgatc cggaccgtac
     2461 ccgcgaacgg gccggtcgtc gatcatccgc agcgccgatg tccagatgtc gatccgtagc
     2521 gagccgctac cgccgccgaa tagatcgagc atgcggtccc gcgcgacggc gacggccaag
     2581 gccaggccgg cgatgacaga cccggcaccg gcgagcctga tccgtggcgg tgcgttgacc
     2641 agcacgatcg cgacggccac aagcccggcc agcatcgccc cacgtgagaa ggtcgcggca
     2701 accgtcacgg cgcagatgac gagcaacggg atgatccaac gccgcacacg gggatccaca
     2761 acggcccaga cgaaggcgag agcggcgacc cggaccgtca tcagggcgag ggcattggga
     2821 tgcggatacg ggccggcgat gcgcgtcacg ccgccgaccg cgaccccgcc gccgagcgcg
     2881 tagtcgccga gtgcgtgcag cgacgcgacg gtgccgccgg cgataagggc aatcgcgacg
     2941 agttcccgca ggataggatc gcgccgatgc cagatcagca gacccagcag caacgccggc
     3001 tcgaacagcg tccagcgcca ttcgcgcagg ctgtcggcgc ggtgggtcgg gtcatacggc
     3061 attagaacga gcagtccacc cagcccgacg agtccgactg ccgcgatcgc gagcggcgac
     3121 ctgagcaccg gtaggagtcg ccgctgcacc ttgcgaagtg cacctgctgg tgactcaacg
     3181 gctgcgacca acacctgcaa ccctgtccca agcaacgcgg cgaagagcag cagttcagaa
     3241 gctgctacgt gctgtccggc gatgtcgagc gggcgataga agtagggcag gctggcgaca
     3301 gcggcggcga tcgcaccgac cggggagaag caggccagcg cgacagcgac gatcaaggcc
     3361 aacagcgcca acgggctgaa gccggtcagc ccggccaacg cgaacgccag gcagcttgcg
     3421 acgcagacgg ccagcgcagg ccgggcgtca gcggtcgcga gcgaagacac gggggtgtcc
     3481 tgttctcgac cggcggcgtg gcgggccgga tcggcggcga atggcagcga ttgccgtgta
     3541 gcaacgctcg gagagggcgg cgacagctgc cagcagcacg actgcggccg ccatcgtcac
     3601 cgtccaggcg gtgggcggct tgccggtcac gaacagcccg gagatcgtca cgctttgccc
     3661 ctcctcggtg acaacctgca tgatgagccg gtggtgcgcg gccggcaggc cggtggcaac
     3721 cggtattgcg gaatcgcgcg cctgggtgct ggagagatcg aggatggcgg ttggcggagc
     3781 cgtctgcgat gccggatcga gccagacgtt gatgaggccg ctgtccggcg actcgaccgc
     3841 gatcaacgag atgtcggtgc cgacgaactc gacttcgagc gccgcgccgg aagcagccgc
     3901 gcgcagatac tgcgtcgtcc cgaccgttga atgctgccat ccgtcattga tctgcatggc
     3961 cggagccttg acgccggaga acccttctgg caactcgtca ccggcgaggg cgacgggggc
     4021 gatcgtggaa atcagcgccg ctggatcgac tccgtcgatc ggcgcgacga agaccagtgc
     4081 ggtgtgagac gtatcggcca gcgcaccggc cacccgttcg acgtcgtcgg ttgacagcgg
     4141 ttcggaggct tcgaacgaat agatcgtgta gcgatcgtgc gcgttcaggc tgagccgatc
     4201 tgggtccagt tcgcggacat tgtcgatcgc gtcgagtgtg atcagcggcc caccggcgag
     4261 agggatcagc gtgtcatcgg acgcgcgcac ttctgcatcc ggccgggctg cttcgtcgat
     4321 gttcccctgc cagccgggca cggcgtccgg atcgcaggct cgcacgtaaa tcacctgatc
     4381 aggaaatcgc gccttgatcg actcgacggc agcctgggcg atcgcgaggc caccgaccgt
     4441 ctcggccggg cgacgcaggc agaacgccaa tggcgagccg cccgccgccg cccgcaatgt
     4501 ttcaatgccg gccaggccgt tctcgcgctg atcttcgagt gccagcgact gcacgacatc
     4561 ggcgtcgacc ggtaggacca tcaacgcgag attcgggttg ccggctgcgt tgagccagtt
     4621 cagggcatcg acggaggaga gcgcgtcgag gttgaggccg aagactcgtt gtcgctcgaa
     4681 cggcaacggc gagtcgggcg gtatcagcag tccgccgcct ctggagccct gctcggcgag
     4741 gccgaggcag acgaacgcag cgacagccag tgtcaggacg gtccagctcg cgcgccgcca
     4801 gagggctgga tcgaatgacg gaatcacgcg ttacctcgcg gccggtctgg ttgcgtcggg
     4861 gcgcggcgaa ccgaaacgac ttgcatggtt cgcgattgta ccaatggcag gcatccggcg
     4921 atggcttgag tacacttcgc cagtggattg gagtgggcga tgcgacagcg gcaaccggcg
     4981 ctcggcatta tcggcggcag cggtctttac ggcctgagtt ctctcacaaa cgtcgaaacc
     5041 gtcgaggtcg aaaccccctg gggaacgccg tcggaagcga tccggatcgg tgagatcgcc
     5101 gggcagacgg tcgcgttcct gagccggcac ggcgccggtc atcgcatttc gccgaccgag
     5161 ataccctacg ccgctaacgt ctgcgcgatg aagatgctgg gcgtcagccg tctcgtctcg
     5221 atcagcgccg tcggcagctt gcgcgaggac tgcccgccac gctccttcgt cgtcccagat
     5281 ggaacgatcg accggacgct gacgcggcag cggacgtttt tcgacaacgg catcgtcgcc
     5341 cacgtctcga tcgccgatcc gttctgccca acgctctcgg cactgttggc gagcgtggcc
     5401 gaaacgggcg gcttgccggt ccatcgcggc gggacctaca tcgcgatcga ggggccgcag
     5461 ttctcaacgc gggccgaatc ggcgctgttt cgatcgtggg gcgcgagcgt gatcgggatg
     5521 acggccatgc cggaggcccg gctggcccgc gaagcggagc tctgctactc ctgtctcgcg
     5581 atggtcaccg actacgatgt ctggcatgag gaggacggac cagtcacggt tgatgcggtg
     5641 ctcgccaacc tgttcgcgat gacggacgcg gtccagacga tcgtgacgac gctggcgggc
     5701 gaggctctgc cggattgcga cgcaggctgc agcgacgcgc tcagcaacgc aatcgccacc
     5761 agccccgacg cgatcgacgc tgccacgcgg gcgcggctcg cgccgatcgc cggccgctat
     5821 ctcgacgcgg agagcgcagg ctgatgctcc agcgcatccg ctttacggaa ctccagctgc
     5881 tgatcgtccc atcagcgatg acaatcgttg gcctgctgac gatctacctt gcctcgactc
     5941 gcgacctcgc atgggaatgg cgcgacatct gggtctcgct ggccttcatg ggcgccgtct
     6001 tcttcatctc catctggctc agcgcgatcg gctttcaagg cgatcaggtg ctgttcccgg
     6061 tcgtcgtctg tatttctggg ctcggcctgc tggtcataca gcggctggcg cccgacctga
     6121 acgagatcga tccggccgca tatggcaacc tggcccgtaa tcagatcatc tacctgacgg
     6181 ccgggctgat cctgatgcta ggcgtctgca cgtttgtccg ccagatcggc tggctgcggc
     6241 gctacaagta cacctgggcg ttcggcggca tcggcctgat gctggcgacg atggtgttcg
     6301 gaaccgagta ctacggtgcg cggctctgga tcaacgtcgg cccgttcagc gtccagccgt
     6361 cggagatcgc caaggtcgca atggtcgtct tcctggccgc ctacctggct gaaaatcgtg
     6421 acctgatcag ctccaattac cggatcgggc cgttctcgat cccgccgctg ccctatctga
     6481 tgccgatgct gctgatgtgg ggcttctcga tcctgatcgt cgtcgcccag aacgacctcg
     6541 gaacggcgtt gctcttcttc ggcgttttcc tctcgatgct gtacctcgcc agcggccggc
     6601 tgctgtatgt ggtcaccggc ctggtgacgt tcgcgattgc cgtctacatc gcctacaaca
     6661 tgttcgaccg aatcggcgtc cgagttgccg gctggctaaa tccctgggca gatccactcg
     6721 acaccggctt ccagccggtc cagtccgagt acgcgatggc gaccggcaag ctgttcggaa
     6781 cggggctggc ccaggggcat ccgaccttca tcacccaggt ccacagcgac tatatcttcg
     6841 ccgcaatcgg tgaggaacta gggctgatgg ggacgttggt cgtcctgctg ctgttcctgt
     6901 tactggtgtt tcggggtttc tacatcgcgc tcaatgcgcg ggacacgttc gggcggctgc
     6961 tggcggccgg tctgacgacg attctcgcgc tgcaggcgct gatcatcgtc ggcggcacga
     7021 tccggctgat tccgctgact ggtatcacgc taccgttcat cagcgccgga ggaagttcgc
     7081 tgttgaccaa cttcatcatc gtcggcctgc tgatgcgtat ctccgacccg acggtgacac
     7141 gctgatgacg accttcatcc gtctgtctgc cttcatcggt gcacttctgg ttcttgccta
     7201 cggcttcggc ggcaccgaag agcaggctga caaggtctgg ctgatgctcc tgctggcagc
     7261 cggcatcctg ctggcgatcg cctggtggcc gcgcgggacg cggacgctgc cggttttcaa
     7321 caggacgctg ctgcgctggt cggcgattat cctggtctgc ttcacgctgg tcagcattca
     7381 gctcgtccgg gtccagatcg tagagtcgag ccggacgaca tcgcggatcg aggaagctcc
     7441 caacggcgag atcgccagca accctcgcga acggttgcgt tcactggaga tgcagcgggg
     7501 ccggatcttc gatcgcgaag ggcgggtgct ggccgacacc gtcctacggg acgacggaac
     7561 ctaccagcgc acctggccgg agctttcgac cgcgccgctg ataggctact actcgccgga
     7621 gatgtatggc tcgaccaacc tggaagccgc ctatgacgag tacctgtcgg gtctggaggg
     7681 cggcaatccg gttgaggagt ggctgaacgg catcctgcac gagcagcagc acggttacga
     7741 cctcggcctg acgatcgacc tcgacctcca gaacctggca gcagagctga tggacggccg
     7801 gcccggcgcg gttgtcctga tggacgctga caccggcgag gtactgacca tgtatggcgc
     7861 gccgtcgttc gacccgaatc agctctacgc caacctgggc cagcaatcca gcgaggaaat
     7921 tgatgcgatc gagaactact gggccgagct gaacgccgat ccgaacgccc cgctcgtctt
     7981 ccgaccgacc gacggcctct acaaccccgg ctcgaccttc aagtcggtga cggcgtcggc
     8041 cgtcatcgac tcaggcacat cgacgctgga gacagtctac cgcgacgagg gggtcttcga
     8101 ggtcgatggg cgggtgatcg aagaagccaa ccggcccgac ccgaacaagg tcaactggac
     8161 gctggaagag tcgtacgcct actcactcaa cgtcgtcttc gcccagatcg ggttgcaact
     8221 cggcagtcag gaactctggg actacgcgca caagttcggc ttcggcgagt cgatcccgtt
     8281 cgacttcgac accgcgacaa cgcagatcgc caattcgcgc gacgatctga ataacagtgc
     8341 gctgctggcc gacaccggct tcggtcaggg gcagattctg gcgactccgc tacaaatggc
     8401 gatggtcgtc tcggcggtcg tcaacgacgg ggtcatgatg cagccgcacg tcgtcgacca
     8461 catccaggag caggacggcg acgtgctgga gacgttcggg gccgatgaat ggcgacgggc
     8521 gatctcggcc gacagcgcgg cggccgtccg caatctgctg ctggcgacgg cggactacgg
     8581 ctattcgcag ggcgcattga ttgatgggct gacggtcggc ggcaagacgg gcacggctga
     8641 ggtcggcgac ggtgagccac actcctggtt caccggcttc gctgagagcg gtgatcggac
     8701 gctggtcgtc tcagtcatcg tcgagcacgg cgg
//
LOCUS       DASUGJ010000040         8863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_283304, whole genome shotgun sequence.
ACCESSION   DASUGJ010000040 DASUGJ010000000
VERSION     DASUGJ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283304"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1804)
                     /locus_tag="VFV93_01030"
     CDS             complement(146..1804)
                     /locus_tag="VFV93_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5163949.1"
                     /translation="METRRTFLKQLMALGIAAPLAGSLAEFEAAFGQAGAGASRGLAQ
                     AGLTSRQMAGQRVICSYAGLTVPEGLLEQIRAGEAAGVIFFGENVESSEQIAGVIQQL
                     QQAREQSPVTSPLLMMTDQEGGLVRRLPGGPERSAKEAGQAADPSGAAAQAGREAALT
                     LSGAGMNVNLAPVLGVYREAGDFLNQYDRSFSNNPDIVSACGSAFITAQQQERVAATA
                     KHFPGLGTASADENTDLRPVTLPVSLSTLRDVDESPYPAAIAAGVKLVMLSWAIYPEL
                     DADRPAGLSATIVQDELRGRHGFGGVTITDALEAGALSAFGSWGERAVLAAGAGMDLI
                     LCSARVVGQGQEAVTALADALDNGRLDTSDFSASIQRVAALRESLADSRFFPETGHTV
                     SLGFLAYWDRFGGLPVFGYPITDSYTDPDTGFVTQYFERARFEWQPGTWPERYDVALG
                     LLGVELAERNGLLGTAPFQPVDGHNDANCSFFPETGHRLCFGFRDFWNANGGLAIYGY
                     PISEEYEEGGYTVQYFERQRLEYHPENSPAWQVEGGLLGSLLLP"
     gene            1987..2886
                     /locus_tag="VFV93_01035"
     CDS             1987..2886
                     /locus_tag="VFV93_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163950.1"
                     /translation="MNARNADEQARIVVQDFTSADDAIRAIQGLKSVGFAERDIDVVA
                     HDDALEQSIRDQAFPDEPEGPLSALASAMRSLTQRLIHRGVTTERALEYSGKVEAGHI
                     LVAVTAGSRAAMARDVMREGLAYTGHMHATERRHPYLDSSTGSGAGVATSLGTATGIT
                     PGSGYEGNTGSTAESSAGVLGQYAPGADTNNPNDDFPGHEAHVEGDWRGMTASEANAL
                     DYRAMMGDGDGETPLQDVAPEGSVDAIETGIFNTGSGSTGNPILDMSIGGTAGITPSI
                     DSLIGQEEGEGYTDPEDDDEEEG"
     gene            2989..3129
                     /locus_tag="VFV93_01040"
     CDS             2989..3129
                     /locus_tag="VFV93_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163951.1"
                     /translation="MLDRAARKYLNMSGEEFVRRYRAGDIPDPDRSEVIRLSILMQYA
                     EE"
     gene            complement(3165..3590)
                     /locus_tag="VFV93_01045"
     CDS             complement(3165..3590)
                     /locus_tag="VFV93_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163952.1"
                     /translation="MVTPSIQHEKFQASSDYLELVVAYPLIAIRTDAQLDDAVAMIDS
                     LLDRGALSEGEEQYLDALSVLVEAYESEHITFPAVSGIDVLKHLMEENELRQSDLLDV
                     FETRSVASEVINGKRPLTMNHVRRLSERFRLPAEVFLGS"
     gene            complement(3592..3885)
                     /locus_tag="VFV93_01050"
     CDS             complement(3592..3885)
                     /locus_tag="VFV93_01050"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEX5163953.1"
                     /translation="MHVISRKKLREFATQHADAGDWLDTWFYTVNKARWFSIEDVHEV
                     YASADVVGKWTVFNVRGNHYRLITEINYMKQRVYIRHVLTHAEYDRGRWKRET"
     gene            complement(4021..6909)
                     /locus_tag="VFV93_01055"
     CDS             complement(4021..6909)
                     /locus_tag="VFV93_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5163954.1"
                     /translation="MRLALYGREAEVALLRECLSEALAGRGRLVLIGGEAGIGKTALV
                     EALAGEARERGALVLAGGCYELAMAPPYGPWSEALAGDLPRDRLASIPPLAGTDGTSP
                     ALSQDVLFSQMLEFFRELAGLTPLVVVLEDQHWAEAASLELLRFVSRSLRDLRVLFLV
                     TYRDADLSAQYPLYRYLPQFVREARPLRISLRRIASESIRQLVVDRYDLPAADEARLV
                     QYLDTYAEGNPFFTEELLRSLVHDGLLASSEDGWQLGDLSDLQVPLLIRQIIDARVSW
                     LDSEAQSLLQLAAVIGIEVPLALWREVSGVDDETLHRVTEQALAARLLVELPGGAGLR
                     FDHALIREALYDGMLLTRRLVWHRRIGEALAARPNADPDAVAHHLERAGDSRAARWLV
                     NAGERAGRARAMQDAIQRYEQALRLLERDDSAAGERGWLLCNLAEAYRYTDPHRALGY
                     LEQAAWVTEQVDDNALEAIVIWSRARIRGFLGENTLAELQQAVEAYDALSPDDHERVL
                     AMGRGYAGSQGPLTQWLGHHGRFDDAIRVGEGCIARQQGELSPAHHSEIAHALFGLGL
                     AYTATGRPDEGRATLERASRHFAGLGNLFMAAAALKWVLLEAVLIYEPVDLDARREVL
                     DEYARLWNKTSTFAAFQDGRMLLPLFPEQFARGDWDDAHEAASAYLRTSFLRIDSLAA
                     LAEIERLRGEHDAAWGRIQLGIPRGIGTEPSSPFFVRTLALQRVAAALALDEGMLDRA
                     REWIDAHDRWLDWSGRLTDRAASLLLRARLELAEGRPDRARELAQAALERARSPRQPL
                     EIQAVQRFIGELETAAGNIDLAALALDDARRLADACDAPYEAALTQFSQAELALADDR
                     PAEAAPLLAAAREVFERLGAAPALDRVAATERQLAPRPTSRSAGGLSPREVEVLQLVA
                     KGMTDAEVADALYISPRTVARHLQSVYNKLGVNSRTAATAFAFEHGLV"
     gene            7257..8684
                     /locus_tag="VFV93_01060"
     CDS             7257..8684
                     /locus_tag="VFV93_01060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5163955.1"
                     /translation="MSIAERARSITGEQIVISDEALEALIAGIRGQVIRPGDAGYDET
                     RLVWNGMIDKYPALIVRASGTADVAATVRFACENELLLTVRGNGHNVAGNAIADGALM
                     LDLSTMRAVQVDPAARIARVQPGANWGDLDRETQLHGLATPGGQVSTTGIAGFTLVGG
                     MGYIRRKYGLACDNLIGAEVVLADGSVVYTSETENSDLLWGLRGGGGNFGVVTSFEYR
                     LYPLGPEVAFVSTIYALDDVEQVLRGWRDYSEQAPDEVTPDFYVWGMPAAPDVPAEMV
                     GMPIVFIAGMYAGPADEGERMLKPLRELATPIADQSGIAPYLQVQCASDSLFPNGLLN
                     YWKSVTLKTLDEDGIKAVLRVSHERPSPQTLIALRYLGGAISRVPEEATAYGNRSALY
                     NLSIDATWTDPFRSEEMIGWTRRVWSEIQQETGAGVYLNFAGFAEENSKLAELAYRSN
                     HDRLVALKRAYDPTNLFRTNVNIRP"
     gene            complement(8674..>8863)
                     /locus_tag="VFV93_01065"
     CDS             complement(8674..>8863)
                     /locus_tag="VFV93_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163956.1"
                     /translation="CRCPRKVGAGLVPVLLPNANRRTGQRTSLPNASGIEQGRHETGP
                     YFSACPRRLADWWLDATA"
BASE COUNT         1567 a         3100 c         2823 g         1373 t
ORIGIN      
        1 ctactggaag cgcgcgctcg ccaactccga ccatcatgtg cccctggagt agcggcgtga
       61 cgcgtgatgc gtgatgcgtg acgcgatggg ctcggagaca accccggtcg tgccaacctc
      121 cgcggcacgc gtcacgcatc acgcgtcacg gcaacaacaa gctccccaac aacccgccct
      181 cgacctgcca ggcgggcgag ttctcggggt ggtattccag ccgctggcgc tcgaaatact
      241 ggacggtgta gccgccctcc tcgtactcct cgctgatcgg gtagccgtag atcgccaggc
      301 cgccgttggc attccagaag tcgcgaaaac cgaagcagag ccggtggccg gtctccggaa
      361 agaagctgca gttggcgtca ttgtggccat caaccggctg gaacggtgct gttccgagca
      421 acccgttgcg ctcggccagc tcgacaccca gcagcccgag cgcgacgtcg tagcgctccg
      481 gccaggttcc cggctgccac tcgaagcggg cgcgctcgaa atactgcgtc acgaagccgg
      541 tatcgggatc ggtgtacgag tcggtgatcg ggtaaccaaa gaccggcaga ccgccgaagc
      601 ggtcccagta ggccaggaag ccgaggctga cggtgtggcc cgtctcgggg aagaagcgac
      661 tgtcggccag actctcccgt agcgccgcca cgcgctggat cgacgccgag aagtcgctcg
      721 tgtcgagccg cccgttgtcg agcgcgtcgg ccagcgccgt caccgcctcc tgcccctggc
      781 caacaacccg cgccgagcag aggatcaggt ccatcccggc cccggccgcc aggacggccc
      841 gctcgcccca ggagccgaac gcgctcagcg cccccgcctc cagcgcgtca gtgatcgtca
      901 cgccgccaaa gccatgccgg ccacgtagct catcctggac gatcgtcgcc gacaacccgg
      961 ccggccggtc ggcatccagc tcgggataga tggcccacga gagcatgacc agcttgacgc
     1021 cagccgcgat ggcagccgga tatggagact cgtcaacgtc gcgcagggtc gaaagggaaa
     1081 ccggcagcgt caccggccgg aggtcggtgt tctcgtccgc cgaggccgtc cccagccctg
     1141 ggaagtgctt cgcggtcgcc gcgacccgct cctgctgctg ggcggtgatg aacgccgacc
     1201 cacaggcact gacaatgtct gggttgttgc taaaggagcg gtcatattga ttgaggaagt
     1261 ccccggcctc gcggtagaca cccagcaccg gcgcaaggtt cacgttcatc ccggcgccgg
     1321 agagcgtcaa cgcggcctcg cgtccggctt gcgccgctgc cccggacggg tcagcagcct
     1381 gaccggcctc cttggccgac cgttccggcc cgcccggcaa acgccggacc agcccgccct
     1441 cctggtcggt catcatcagc aatggcgacg tcaccggact ctgctcgcgg gcctgctgca
     1501 actgctggat gacaccggcg atctgctcgc tgctctcgac gttctcgccg aagaagatga
     1561 cgccggccgc ctcgccggcc ctgatctgct ccagcagccc ctccggcacc gtcagccccg
     1621 cgtaggagca gatcacccgc tgcccggcca tctgccgcga cgtcaacccc gcctgcgcca
     1681 gtccacgcga cgccccagcg cccgcttgcc cgaacgctgc ctcgaattcc gccagcgatc
     1741 ccgccagcgg cgccgcaatg cccaacgcca tcagttgctt cagaaacgtc cgccgagtct
     1801 ccatcaaaag ctgcctccag ctgcctgtgc cacgaaaggt ccggtttcaa cgctacgccc
     1861 gctgtcaacc gccggccgag ccggggcgac gtggaactgg cattgcatac cgcacggcag
     1921 cgcacgataa tacgcggacg cagacaagtg ggaatacaca cgacgaacgg acgtaagggg
     1981 gcgggcatga acgcgcgcaa cgcggacgaa caggcgcgaa tcgtcgttca ggacttcaca
     2041 tccgctgacg atgccatccg ggcgatccag ggattgaaga gtgtcggctt cgccgagcgc
     2101 gacatcgatg tcgtcgccca cgatgacgcg ctcgagcagt cgatccgcga ccaggcattt
     2161 cccgacgagc cggaaggccc gctctcggca ctggcgagcg cgatgcgctc actgacgcaa
     2221 cggctcatcc accgcggcgt cacgaccgag cgtgcgctgg agtacagcgg caaggtcgaa
     2281 gcgggacaca tcctcgtcgc ggttaccgcc ggtagccgtg ccgcaatggc ccgcgatgtg
     2341 atgcgcgagg ggctggccta cacgggccac atgcacgcca ccgagcgtcg ccacccgtac
     2401 ctcgactcca gcaccggatc gggcgcaggc gtcgccacca gcctcggcac agcgaccggc
     2461 atcacgcccg gctccggcta cgaaggcaac accggctcga cagccgaatc ctcggccggc
     2521 gtcctcggcc agtacgcgcc gggagccgac accaacaacc caaacgacga cttccccggc
     2581 cacgaagcgc acgtcgaagg cgattggcgc gggatgaccg cctccgaggc gaacgccctc
     2641 gactatcgcg cgatgatggg cgacggtgac ggcgagacgc cgttgcagga tgtcgccccg
     2701 gaaggatcgg tcgacgcgat cgagaccggc atcttcaaca ccggcagcgg ctcaaccggc
     2761 aacccgatcc tcgacatgag catcggcggc acggccggca tcacccccag catcgactcc
     2821 ctgatcggcc aggaagaagg cgaaggctac accgatcccg aagacgacga cgaggaagag
     2881 ggatagcgca gccgcaaccc tcgtggaggc atgacaattc gcagcgggaa aacatccgag
     2941 taccgaattc ccggagttcg ttacgtgtcc cgcgaggaag gcgtagcgat gctcgatcgt
     3001 gctgcccgca agtacctgaa catgtccggc gaagagttcg tccgtcgcta ccgcgctggc
     3061 gacatccccg acccagaccg ctcggaggtc attcgtctat caattctcat gcagtacgca
     3121 gaagaataat cactcggctg gctacgttcc tcaattcgac ccggttatga gccgagaaac
     3181 acttcagcag gcagccgaaa gcgctcactc aaccggcgga cgtgattcat cgtcagcggg
     3241 cgcttcccgt tgatgacttc cgaggcaacc gatcgcgtct caaaaacgtc cagcagatcg
     3301 ctctggcgca gttcgttctc ttccatcaga tgcttcagaa cgtcgatgcc agagacggcc
     3361 ggaaacgtga tgtgctcgct ctcatacgcc tcgacgagca cgctgagggc atcgagatac
     3421 tgctcctcgc cctcggacaa cgcccccctg tccagcaggc tatcgatcat cgcgacggca
     3481 tcatccaact gcgcatcagt ccgaatcgct atgagcggat aggcaacgac aagctccaga
     3541 tagtctgacg atgcctgaaa tttctcgtgc tggattgagg gtgtgaccat cttatgtctc
     3601 gcgtttccat ctgcctcgat cgtactccgc gtgcgtcagc acatgacgga tgtagacgcg
     3661 ttgcttcatg tagttgatct cggtgatgag ccggtaatgg ttgccacgaa cgttgaagac
     3721 tgtccacttg cccaccacgt cagccgaggc atagacctca tgaacatctt cgatgctgaa
     3781 ccaccgagcc ttattgacgg tgtagaacca tgtatccagc caatcaccag catcagcatg
     3841 ttgcgtcgca aattcccgca acttcttcct gctgatcaca tgcacacgct gtactcctct
     3901 tgccctgata ttcgcagatt gcgaacacca tgtcaagtgt cggacgcaat gcgttcggcg
     3961 aacttggcag cgtggccgat tcgctcatct atcatgaaca tacgcctgac gacgcattga
     4021 tcacaccaac ccgtgctcga aggcgaaggc ggtggcggcg gtgcgggagt tgacgccgag
     4081 cttgttgtag acggattgca ggtggcgcgc gacggttcgg ggggagatgt agagcgcgtc
     4141 agcgacctcg gcgtcggtca tccctttggc gaccagttgg aggacctcga cctcgcgggg
     4201 cgagaggccg ccggctgatc tggaggttgg tctgggtgcc aactggcgct cagtcgcggc
     4261 gacccggtcg agcgcgggcg ccgcaccgag gcgctcgaac acctcgcggg cagccgccag
     4321 tagtggtgca gcctcggccg gtcggtcatc cgccagcgcc agttcggcct ggctgaactg
     4381 ggtcagcgcc gcttcgtagg gcgcgtcgca ggcgtccgcc agccggcggg cgtcgtccag
     4441 cgccaaagcc gccaggtcga tgttcccggc cgccgtctcc agttcgccga tgaagcgctg
     4501 gacggcctgt atctccagcg gctgtcgcgg gctgcgggcg cgctccaggg ctgcctgcgc
     4561 cagttcacgc gcccggtccg gccgcccctc ggctagttcc aaccgggcgc gcagcagcag
     4621 gctggcggcg cgatcggtca gccggcccga ccagtcgagc cagcgatcgt gggcgtcgat
     4681 ccactcacgc gcccggtcca gcatgccctc gtcgagcgcc agcgcggcag ccacgcgctg
     4741 caacgccagc gtccggacga agaacgggct gctcggctcg gttccgatcc cacgcgggat
     4801 cccgagctgg atgcgtcccc aggcggcgtc gtgctcccca cgcagtcgct cgatttcggc
     4861 cagtgccgcc aggctgtcga tccgcaggaa gctggtccgc aggtaggcgg aggccgcctc
     4921 gtgggcgtcg tcccagtcgc cacgggcaaa ctgctccggg aagagcggca gcagcatccg
     4981 gccgtcctgg aaggcggcga aggtgctggt cttgttccag aggcgggcgt actcatccag
     5041 cacctcgcgg cgcgcgtcga gatcgaccgg ttcgtagatc aggacggctt cgagcagcac
     5101 ccacttgagg gccgcggctg ccatgaacag gttccccagc ccggcgaagt gccggctggc
     5161 ccgttcgagc gtcgcccgac cctcatccgg ccggccggtc gcggtgtagg ccaggccgag
     5221 cccgaacagc gcatgggcaa tctcgctgtg atgggccggc gatagttccc cctgctggcg
     5281 ggcgatgcag ccctcgccga cgcggatcgc gtcgtcgaag cggccgtggt gccccagcca
     5341 ttgcgtcagc ggcccctgac taccggcata gccgcgcccc atcgcgagca cccgctcgtg
     5401 gtcgtcgggc gagagcgcgt cgtatgcctc gacggcctgc tgcagctcgg ccagcgtgtt
     5461 ctcgccgagg aagccgcgaa tccgcgcccg gctccagatg acgatcgcct ccagcgcgtt
     5521 gtcgtcgacc tgctcggtga cccaggcggc ctgttccaga tagcccagcg cccggtgggg
     5581 atcggtgtag cgatacgcct cggccaggtt gcagagcagc cagccccgct ctcccgccgc
     5641 gctgtcgtct cgctccagca gccgcagcgc ctgctcgtag cgctggatag catcctgcat
     5701 cgcccgcgcc cggccggccc gctcgccggc gttgaccagc cagcgggcgg cgcgtgaatc
     5761 gccggcccgc tcaagatggt gggcgactgc gtctgggtcg gcattcggcc gcgccgccag
     5821 cgcctcgcca atccggcggt gccagaccag ccgccgcgtc agcagcatcc catcgtagag
     5881 cgcctcgcgg atcagcgcat ggtcgaagcg caaccccgcg ccaccgggca gctccaccag
     5941 cagccgcgcc gccaacgcct gctcggtcac gcggtgcaac gtctcatcat cgacgccgga
     6001 cacctcgcgc cagagcgcca gcggcacctc gatcccgatt acagccgcca gctgcaacag
     6061 gctctgcgcc tcgctatcca gccagctgac ccgcgcgtcg atgatctggc ggatcagcag
     6121 ggggacctgg agatccgaca ggtcgccgag ctgccagccg tcctccgagc ttgccagcag
     6181 cccatcatgg accagcgagc gcaacagttc ctcggtgaaa aatgggttac cctcggcgta
     6241 ggtatcgaga tactggacca gccgcgcctc gtctgccgct gggagatcgt agcggtcgac
     6301 gaccagctgc cggatcgact cgctggcgat ccgccgcagg ctgatgcgca gcgggcgcgc
     6361 ctcgcgaacg aattgcggca ggtagcggta gagtgggtac tgcgccgaca gatcggcgtc
     6421 gcggtaggtg accaggaaca gcacccgcag gtcgcgcagc gagcggctga cgaagcgcag
     6481 caactccagg ctggccgcct cggcccagtg ctggtcctcc aggacgacga cgagcggcgt
     6541 caggccggcc agttcgcgga agaactccag catctgcgag aagagcacat cctggctcag
     6601 ggccgggctg gtaccgtccg tgccggcaag tggggggatc gacgccaacc ggtcgcgcgg
     6661 gagatcgccg gccagcgcct cgctccacgg cccatacggc ggcgccatcg ccagctcgta
     6721 gcagccaccg gccagcacca gcgcgccgcg ttcgcgggcc tcgcccgcca gcgcctcgac
     6781 cagcgccgtc ttgccgatgc cggcctcgcc accgatcagc accagtcgcc cccggccggc
     6841 aagcgcctcc gacagacact cgcgcagcag ggcgacctca gcctcccggc catatagcgc
     6901 caggcgcatt ggggaacgtg acacgggcgg ggaatgggaa agtgtcatgg tcgtgacagt
     6961 cccgtccgag agatcctgga tcgtcgcaag atcgccgctg tcgctatcgc agcatggttt
     7021 gactatagca cgcttgtggg gacgcgggac gcggggtggt tccttgcgac cggcgtcccg
     7081 catcccgtgg ttagttctac ccatcccctt ggtgccggcc ctgctccggg cagaatgggt
     7141 gatttctgcg atggcgttgc gttcgatgac gcctacgctg tctgccgttg gcaatggctg
     7201 ccggcgacga gcacccagca ccgcgcactg acgcgcggga tgaaaggaac agatcaatgt
     7261 cgatcgcgga acgcgcgcgc tccatcaccg gcgagcagat cgtcatctcc gacgaggcgc
     7321 tcgaagcact catcgccggg attcgcggcc aggtcatccg ccccggcgac gccggttacg
     7381 acgagacccg gctggtctgg aatgggatga tcgacaagta cccggccctg atcgtccgcg
     7441 ccagcggcac ggccgatgtc gccgcaaccg tccgctttgc ctgtgagaac gagttgctac
     7501 tgaccgtccg gggcaacggg cacaacgtcg ccggcaacgc gatcgccgac ggcgcgctga
     7561 tgctcgatct ctccacgatg cgcgccgttc aggtcgatcc cgccgcccgg atcgcccgcg
     7621 ttcagccggg cgccaactgg ggcgacctcg accgcgagac gcaactgcac ggcctcgcca
     7681 cacccggtgg tcaagtctcg acgaccggca tcgccgggtt cacgctggtg ggcggcatgg
     7741 gctacatccg tcgcaagtac ggactggcct gcgacaacct gatcggcgct gaggtcgtcc
     7801 tggccgatgg ctcggtcgtc tacaccagcg agaccgagaa tagcgacctg ctctggggat
     7861 tgcgtggcgg cggcggtaac tttggcgtcg tgacgtcgtt cgaatatcgc ctctacccac
     7921 tcggcccgga ggtcgccttc gtcagcacga tctatgcgct cgacgatgtc gagcaggtcc
     7981 tgcgcggctg gcgcgactac agcgagcagg cgcccgatga ggtcacaccg gacttctacg
     8041 tctggggcat gccggcggcg ccggacgtcc cggccgagat ggtcggcatg ccgatcgtct
     8101 tcatcgccgg gatgtacgct gggccggcag acgagggcga gcgcatgctc aagccgctgc
     8161 gcgagttggc aacaccgatc gccgaccaga gcggtatcgc gccatacctc caggttcagt
     8221 gcgcctcaga ctcgctcttc ccaaatggcc tgctgaacta ctggaagtcg gtcacgctca
     8281 agacgctcga cgaggacgga atcaaggctg tcctgcgtgt cagccatgag cggccgtcac
     8341 cacagacgct gatcgccctg cgctatctcg gcggcgcgat cagccgcgtc ccggaggagg
     8401 cgaccgccta cggcaaccgg tcggccctct acaacctcag catcgacgcg acctggaccg
     8461 atccgttccg gtcggaggag atgatcggct ggacgcgacg tgtctggtcg gagattcagc
     8521 aggaaaccgg cgccggcgtc tacctcaact tcgccggctt cgccgaggag aacagcaagc
     8581 tcgccgaact ggcctaccgc tccaaccacg accggctcgt cgccctcaag cgcgcatacg
     8641 acccgaccaa cctgttccgc acgaacgtga acatcaggcc gtagcatcta gccaccagtc
     8701 agccaaccgc cgaggacatg ccgagaagta ggggccggtc tcgtgccggc cctgctcaat
     8761 ccccgacgcg ttcggcagcg acgtgcgttg tcccgtacgg cggttcgcgt tggggagcag
     8821 gaccggcacg agaccggccc ctactttccg aggacatcgg cag
//
LOCUS       DASUGJ010000041         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_283309, whole genome shotgun sequence.
ACCESSION   DASUGJ010000041 DASUGJ010000000
VERSION     DASUGJ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283309"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="VFV93_01070"
     CDS             complement(<1..1487)
                     /locus_tag="VFV93_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEX5163957.1"
                     /translation="MTSRSALIFQRDGKLLVTQGEGLDEIIRDIRQCAQLRSIVAGNH
                     VYELSPSALWGLAARGVGANSLLDQLERYAAMPVPAPMAARIAETMGRVGALEVFDGT
                     DGVRIRARTPAILREIGIDSPNGDSPVVRIEPSTLGRIKLAAVEAGWPVIDRRRLLDR
                     ASPIVLNEDVSLRPYQLGALAAFRNGRDGVVLLPCGAGKTLVGVAAAMETGGPVLVLV
                     PSRTVGEQWQAAFRDATDCPPSDIGWFSASDPQVVSTVTYHAATSGHGTGDLTGRRWR
                     LVIFDEVQSLPADVFRLASGIDADRRLGLSATLVREDGREAEVFALVGPALYEASWLE
                     LERDGWIAPARCVEVRVPASTSTVDHWRYKMAVVQRLLERHANVPALVVGTDVASLRA
                     AGRRLGFPVLTGSSSNERRAQVLDAFRAGLIQTIGLSRIGSVGIDLPDASVLIQVSGT
                     FGSRQEEAQRLGRILRPASGKTAHFYTIVAAGTREQHFASRRQRFL"
     gene            1580..>2110
                     /locus_tag="VFV93_01075"
     CDS             1580..>2110
                     /locus_tag="VFV93_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5163958.1"
                     /translation="MEIGNIRPVNIDSEMRRSYLDYAMSVIVQRALPDVRDGLKPVQR
                     RILYGMSEMGLRANTRYRKSAGIVGEVLKSFHPHSDTAVYDALVRMVQDFSLRYPLAQ
                     GQGNFGSVDGDGAAAMRYTETKLSAIAEEMLIDIDKETVDFKPNYDDQTREPKVLPAK
                     LPNLLVNGASGIAVGMA"
BASE COUNT          402 a          711 c          648 g          349 t
ORIGIN      
        1 aggaagcgtt gccgccggct ggcgaagtgc tgttcgcgcg ttccggccgc gacgatcgtg
       61 tagaaatgcg ccgtcttgcc ggatgcaggg cggaggattc gtcccagccg ctgcgcctct
      121 tcctgccgcg acccgaaggt acccgacacc tgaatcaaca cactcgcatc gggcagatcg
      181 atgcctaccg atccgattct cgatagacca atcgtctgta tcaaaccggc acggaacgcg
      241 tcgagcacct gagcacgccg ctcgttgctc gatgatccgg tgaggactgg aaatccgagc
      301 cgccggccgg ctgcgcgcaa gctggcgacg tcggtcccga cgaccaaagc cggcacattc
      361 gcgtgtcgct ccagcaaacg ctggacgacg gccatcttgt agcgccagtg gtcgaccgtc
      421 gatgtcgacg ccgggacgcg gacctcgacg caacgggcgg gagcgatcca gccatcgcgt
      481 tcgagttcca gccaggacgc ctcgtacagc gccggcccga ccagcgcgaa gacctctgcc
      541 tcgcggccat cctcgcgaac caacgtcgcc gagagcccaa gccggcgatc ggcgtcgatg
      601 ccggacgcca gtcgaaacac gtcggccggc agcgactgaa cctcgtcgaa gatcacgagc
      661 cgccagcgtc gtcccgtcag gtcgcctgtg ccgtggcccg atgtcgcggc gtgataggtc
      721 acggttgaga caacctgcgg gtcggaagcg ctgaaccaac cgatgtcgct cggtgggcaa
      781 tcggtcgcgt ccctgaaggc tgcctgccac tgctcaccga ccgttctgga cggcacgagg
      841 accagcaccg gcccgcctgt ctccatagct gccgcgacgc caacaagcgt cttgccggcc
      901 ccgcatggca acaggacaac gccatcccgg ccgttgcgga aggcggccag cgcaccgagt
      961 tgatacggac gcagcgacac gtcctcgttc agcacgattg gagacgcgcg atcaagcagt
     1021 cgtcgacggt ctatgaccgg ccacccggcc tcaaccgccg cgagcttgat cctgcccagg
     1081 gtgctcggct cgatacgcac gacgggagag tcgccattcg gggagtcgat gccaatctcg
     1141 cgcagaatgg ccggcgtccg cgcccgaatg cgaacgccgt cggtgccgtc gaagacttca
     1201 agtgcaccga cgcgacccat cgtctccgcg atccgggctg ccatcggcgc cggaaccggc
     1261 attgcggcgt accgttccaa ctggtcgagt agcgagttgg caccgacgcc tcgcgcggcc
     1321 agtccccaga gagcggacgg cgacaattcg tagacatggt tgccagcgac gatcgagcga
     1381 agttgtgcgc attggcgaat gtcgcgaatg atctcgtcga gtccctcgcc ctgcgtaacg
     1441 agcaacttcc cgtcccgctg gaagatcagc gccgacctgg atgtcatcgc agcccgattc
     1501 ggcgtacgga cgccaaaact ggtataattg tctggtttga agcgcaccgt actcgccctt
     1561 gaaatggggg tccattcacg tggaaatcgg aaatatcagg cccgtcaaca tcgactcgga
     1621 gatgcggcgc tcctacctcg actacgcgat gagcgttatc gtccagcgcg cccttcctga
     1681 cgtccgcgac ggtctcaagc cagtccagcg gcgcatcctc tacggcatgt ccgagatggg
     1741 tttgcgcgct aatacccgct accgcaagag cgccggtatc gtcggcgaag tcctcaagtc
     1801 gttccatcca cactccgaca cggccgtcta cgacgcgctg gtccgtatgg tacaggactt
     1861 ctcgctgcgt tatccgctcg cccagggcca gggcaacttc ggctcggtcg acggcgacgg
     1921 cgctgccgcg atgcgctaca ccgagacgaa gctctccgcc attgccgaag agatgttgat
     1981 tgatatcgac aaggaaacgg tcgatttcaa gccgaactac gacgatcaga cccgcgagcc
     2041 gaaagtgctg ccggccaagt tgccgaatct gcttgtcaac ggggcgtccg gcatcgccgt
     2101 cggcatggcg
//
LOCUS       DASUGJ010000042         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1672542, whole genome shotgun sequence.
ACCESSION   DASUGJ010000042 DASUGJ010000000
VERSION     DASUGJ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672542"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFV93_01080"
     CDS             <1..624
                     /locus_tag="VFV93_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163959.1"
                     /translation="RALQEGMDEPILHTGIDEYSPHVPVKFTAVTGGDSPTISDYFTI
                     VYDKVPRRIVEQTTFGEVVYGDGVVVDAPVGPKGITLLRAMYAGVAAIAAAGVSLVVD
                     EVLYQPSVLRAAVEELSDSNVLFVGLRLPLQVAEQRERDRGDRGLGAARLFYDRVHLH
                     GIYDLELDTSAMSPDEGARAIKNVLERNQQGTAFQMLAHRFAQSSDS"
     gene            complement(665..1045)
                     /locus_tag="VFV93_01085"
     CDS             complement(665..1045)
                     /locus_tag="VFV93_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163960.1"
                     /translation="MELTYDSATNTLRLTPDNQGVARPLGATKTLEGMLDIGEAGQLI
                     GVEFNATPNELVNWMTGSPDSNAVEVDTNSRAYIQIMSDAGGNTRSTALDLIAEYDTD
                     GRIIALAIPRHGHGYEITYPSGNQ"
     gene            1139..>2041
                     /locus_tag="VFV93_01090"
     CDS             1139..>2041
                     /locus_tag="VFV93_01090"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5163961.1"
                     /translation="MARAGVLDTDERAELTDEADRPAHGGRLARVRDARALAAWGYRP
                     FRRYVGVQVLSMAGRALQSTLIGYMVYELSESDLLLGVVSFMQMVPQLLLAPVVGVIV
                     DRFDRRRILAAQFTSQAVGFLVLALLALGDLLTVPAIAVTVVLMGIANAFSYPAHSSL
                     LPSLVPVRTLQSANAINSMMGNLSRVAAPTVSGLIVDGAGVAAALLLASGIYLPAAGL
                     VLLVPLLLGVEGVSATRGPLQPAARPSFRSDIGDAVGYIKTNPLLRSALVNDIVPYLF
                     GMAHIALLPAIASDTLDGGATMLGL"
BASE COUNT          308 a          584 c          745 g          404 t
ORIGIN      
        1 agagcgttgc aggaggggat ggacgagccg atcctgcaca cggggattga tgagtactcg
       61 ccgcatgtgc cggtcaagtt cacggcggtc acgggcggcg attcaccgac gatttctgac
      121 tacttcacga tcgtgtacga caaggtgccg aggcgtatcg tagagcaaac gacgttcggc
      181 gaggtggttt atggcgacgg cgtcgttgtc gacgcgccgg ttggcccaaa ggggatcacg
      241 ctgctgcgtg caatgtacgc cggagtcgcg gcgatcgcgg cagccggcgt tagcctcgtc
      301 gtcgatgaag tcttgtatca gccgagcgtg ctgagagccg cggttgagga gctttcggat
      361 tcgaacgtgc tattcgtcgg tctacggctg ccattgcagg tcgcggagca gcgggaacgg
      421 gatcgcggtg atcgtggtct cggcgcagcg cggttgttct acgatcgggt ccatctgcac
      481 gggatctacg atctcgagct tgatacatcc gccatgagtc ccgacgaggg cgcccgcgca
      541 atcaagaacg tcctggagag aaaccagcaa gggactgcgt ttcagatgct ggcacaccgg
      601 ttcgcccagt cgtccgacag ttgatccgag tctacgcggc gcccaacggg cgccagggga
      661 cccatcactg gttgccgctg ggataggtga tctcgtagcc gtggccgtgc cgggggatgg
      721 cgagggcgat gatccgaccg tcggtgtcgt actcagcgat gaggtcgagt gctgttgagc
      781 gggtgttgcc gccggcgtcg gacatgatct ggatgtaggc gcggctgttg gtgtcgacct
      841 cgactgcgtt ggaatcgggt gaacctgtca tccagttgac gagttcgtta ggggtggcat
      901 tgaactcgac tccgatgagc tggccggctt caccaatgtc gagcatacct tcgagggtct
      961 tcgtcgcgcc gagaggccgc gcaacgccct gattgtcggg agtcaggcgg agcgtgttgg
     1021 tggcactgtc gtacgtcaat tccatgcagt aatccgatgc atcgcgggcg acgggagcaa
     1081 cgccctcgat gctactacag cgccggtcga aggggtgcta tcgttcgcgg gttgagcgat
     1141 ggcgagggct ggcgtgctgg ataccgacga gcgagctgaa ctgacggacg aggcggatcg
     1201 tccagcccac ggcgggcggc tggcgcgggt gcgggatgcg cgtgcgctgg cggcctgggg
     1261 ctaccggccg tttcggcgct atgtcggtgt ccaggtgctg tcgatggcgg ggcgcgcgct
     1321 gcaatcgacg ttgatcggct acatggtcta cgagctgtcg gagagcgacc tgctgctggg
     1381 cgtcgtctcg ttcatgcaga tggtgccgca actgctgctg gctccggtcg tcggcgtgat
     1441 cgtcgaccgc ttcgaccggc ggcgaatcct ggcggcgcag ttcacgtcgc aggcggtcgg
     1501 gtttctggtg ctggcgttgc tggcgctggg tgatctgctg acggtgccgg ccattgcggt
     1561 tacggtcgtg ttgatgggaa tcgccaacgc gttctcgtat ccggcccact cgtcgctgct
     1621 gcccagcctc gtgccggtcc ggacgcttca gagcgccaac gcgatcaact cgatgatggg
     1681 gaacctgtcg cgggtggcgg cgccgacggt gtcagggctg atcgtcgacg gcgcaggggt
     1741 cgcggcggcg ctgctgctgg cgtcggggat ctacctgccg gcggccgggc tggtcttgct
     1801 ggtgccgcta ctgctgggtg tcgagggagt ctcggccacg cgcggaccgc tgcaacctgc
     1861 cgcccggccc tcgttccgca gtgacatcgg cgatgcggtc ggctatatca agaccaatcc
     1921 gctactccgc tcggcgctgg tgaacgacat cgtgccgtac ctgttcggga tggcgcacat
     1981 cgcgctgctg ccggccatcg ccagcgacac gctcgacggc ggcgcgacga tgctggggct
     2041 g
//
LOCUS       DASUGJ010000043         9757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1950954, whole genome shotgun sequence.
ACCESSION   DASUGJ010000043 DASUGJ010000000
VERSION     DASUGJ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950954"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFV93_01095"
     CDS             <1..203
                     /locus_tag="VFV93_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163962.1"
                     /translation="SLGDSEIAVAIHNLSREPCEVTVDFGEWDRRHLADLFGDRQYEP
                     LTTKQGAIRMEGYGYRWLRAGK"
     gene            complement(285..479)
                     /locus_tag="VFV93_01100"
     CDS             complement(285..479)
                     /locus_tag="VFV93_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163963.1"
                     /translation="MSVIDDDVNQTQRGEPANGNDAEARRRAGSTMDPYTAMKSIEYN
                     QQELARLRGARNWVSLFNKK"
     gene            830..5233
                     /locus_tag="VFV93_01105"
     CDS             830..5233
                     /locus_tag="VFV93_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEX5163964.1"
                     /translation="MSNSRFPRPIDPDGQRKLAPTDISQFIRLDQCDRYLRLRLHERA
                     FGQRFMREYDVTPQSIPALLTRSGSVFEMKTESTVANHFPIENYAARDHESGSRQADN
                     ERLIGAIRDLEPDSVFVVFQPRLEVDVDGWMIRGDVDILRLERDVAGRLTITIADMKS
                     SRSAKVEHRVQVAFYHAMLTRLLATHAIETDTIRMAILYRGPSEADDAQLDDEERQRR
                     EAQRAAARELFGVTDALLEIVEDPEHYLRAVEDLVTGKDSRAIRVAEAPFDHVPYHLT
                     YKCDGCIYSEFCMKWSAERDDLSLIPHLTIQQKSSLRRAGITTTSQLAALKQPVADDP
                     NTLQPAPGHEAQTRALAATWPVGPRLDELIHRARRYRKYVKDPIEAISYLPSKGYGSL
                     PYTDASHNPNLVRIYIDAQHDYLNDRVYLLGALLVGCEDGVESPERRKSVVMMSDGPP
                     ATAEDERHLLVGWIGGVLATLEEVAAPNEEGLPKAPIHLIFYDRMQQTMLLDALSRHF
                     ASIVEATPLFDFITQIAAFDSPVATFLEDEIRELKNYPMVCQSLQSVAAYLRFDWNQP
                     EEYRRLFSVRLFDFWGKLEQENGEMAWYTNRARFNSMIPLEYAYAAWGDLDVPADADP
                     GEYRRFGGVDATLIQGFQRRRLEALEHIARDFRGNKQTVKSAFDIPNLAEFDDRSREM
                     ANALREFLTIERHTELTAWKNARLAPPERRVLAGDTLLVRYVEADQEPGVAALNRDNR
                     RRMELREKYRAAYAAEHPDASRITLPKEQRQESDWSQDGIGFWLRVETDGVDCDLEEI
                     LSLTSFRERDSLVLFPRWAVDSRLPEDERYEFTPTPKQLLYGMRVILKQIVIDRPAGG
                     RAKSARVLVEMQNGQGRGQNGFVFGTTPFNNLPLEQGKVYTLDSNPTDWYGSHCVEVV
                     KGLIEGGVNTLFDWLSDTAALAGARHDWPLAAAEGQRRFLAGLDALGAAGALHGFEPG
                     KRDYIAGHGDAPILLVQGPPGTGKSYSTAFAIFARIQGAMAAGIPFRVFLTCKTHAAT
                     DVLLDNVLNVQRKLADWFIEQPEIAEQFFDRRLTQVPLFRIRPRGDVAGLIVPLLDDR
                     NKPAGKKAIDQIMAQEWAIVAAAPAGTRSAIKDKWSKEIFGHEVADCLILDEASQMNL
                     PEAILAGLPLKQDGQLIVVGDHRQMPPIVKHDWSSEPRRTFQEYRSYESLFEALLPVG
                     LPIIRFEESFRLHAEMAEFLRREIYQHDQIPFHSKRTTTLGPIAHNDPFVAAVLDPAY
                     PLVVIVHDEAGSQLQNDCERELITPVLHALATIPLVNGHDNLPRNGLDPYVGLGVVVP
                     HRAQRAAFIEHVPSVTERDKDTGTIRKSAVDTVERFQGDERQVIVYTATESDPQYLVA
                     ASKFLMDPRRLTVALSRAKSKIIVVASRSVFSIFSADEETFANAQLWKNLLRETCIVP
                     LWSGPRTANGVEVGVEVWGNQPGVTQQALTP"
     gene            complement(5208..6134)
                     /locus_tag="VFV93_01110"
     CDS             complement(5208..6134)
                     /locus_tag="VFV93_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5163965.1"
                     /translation="MSEIRTIMAEMRKKRRKHDAELAQITETQMGIQTAFTFETMMTG
                     GQGKTTGAAVRDIFLRRPDHLEEHAIQIEGILRGRFGVERTQAHLIWAANQTAHGDLH
                     AALTGLTNDDLDSREGLPDGEWALRQILEHLIVVERYYTLDIHHALKQFHAGEPHGDL
                     PDDELQVERPGATLAQLIQDHDDVRDEALTALADLTEAELRAPARWGEIAIDVRFLLM
                     RFAHHEREHTDQTLKWRLQRGRQQTEAERLLGLCWRQSGILEGHLIGAPEDILDRDPG
                     DGEWLVRRILAHIGSAETYFRRVILKALEPVG"
     gene            6297..7919
                     /locus_tag="VFV93_01115"
     CDS             6297..7919
                     /locus_tag="VFV93_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5163966.1"
                     /translation="MASADVVFVNGLVRTLDPGNPVAEAVLVRGGRIAAVGDLTEVRG
                     QASGAREVDLGGATLLPGFVESHNHFIDAGQAFAQVDCRGERVGSIAELQQALRERAE
                     VTPAGNYVLGRGYDQTLLAEDRHPTRDDLDAAVPDHPVIIWHISYHGLVANSAALRRA
                     GVDRNTADVDGGEILRDEHGEPSGVFLESPAMSLVTAPLGEPTVATLRAGLAHARDQF
                     VPTGISTVHDALIATRVKIDAYAEAMLAGELPIRVYMMIAPELYAGALEGQAGDLGPS
                     YGLPAGRLVAGPVKLFQDGAIQAYTASLTEPYFDAPDRYGVKIYEPAHLAEMVLEHHL
                     AGRQIAIHGNGDSAIDDILNAYEAALAAKPRADHRHRIEHCQTARQDQLERMARLGVV
                     SSVFANHLWQFGDRHIARFLGPERAARMEPLASMTELGIRWGLHSDCPVTEVNPLRGI
                     WLAVNRRTSSGQPIGERERVSAEQALRGFGPDAAFLGFQEQQLGTIAPGYFADFVVVS
                     FDPVADPEGIKDLDVLATVVGGEIVHQSPIAN"
     gene            8046..>9757
                     /locus_tag="VFV93_01120"
     CDS             8046..>9757
                     /locus_tag="VFV93_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5163967.1"
                     /translation="MSDAGNTVTDAEAVSRQDGERARMQQAVAQLGQRALVSSDLEAL
                     MDAVVTIVADVLAVEFCKVLELLPDGKSLLLRAGTGWNEGVVGQAIIGVEDDSQAGFA
                     LMSSQPVVVNDLRVESRFNGPRLLVEHGVISGMSVIIHGANDPWGVLGAHTARPRTFN
                     DDDVNFIQSVANVLAAAIDRSLAEDALSESEERFRTLVEMAPDVIFSVEVTDGTFTSL
                     SPAFEQLTGWSRSEWLGRSFTEIVHTDDLPLAYHHFQRLLAGQLPPPFELRVTSRDGR
                     EIIGEFRVTPQVEGGQVVALFGIARDVSDRRRAEEELRRSRDELEIILRGVADGITVQ
                     DATGALVYANETAARLIGCATVEELLTTPTTELLRNYEMFDEAGAPFPLDRLPGRLAL
                     LGQPSSEVLMRSRVITTGEERWSVLSATPVPGPDGAPRFAINIFHDVTERMRTERVHD
                     FLARATALLSATLEYEQTLDRVAHIAVPFLADLCVFDIVRPDGSLRRIAAHEDPETER
                     LMHQLINQHPIEPGHPVMLAIQTGKVTLHVDIASEAVARAGLTDARVEVIAALGFRSV
                     IVVPL"
BASE COUNT         1660 a         3113 c         3266 g         1718 t
ORIGIN      
        1 cgagtctcgg cgattcggag atcgccgtcg cgatccacaa tctgtcacgt gaaccatgcg
       61 aggtcacggt cgacttcggc gagtgggacc ggcggcatct cgccgacctg ttcggcgacc
      121 ggcagtacga gccgttgacc acgaagcagg gagcgatccg gatggagggg tacggctatc
      181 gctggttgcg ggcggggaag taggtccagc cgtccagaca cgagtgaggc gagcactgct
      241 gctcgcctca tcagcctgat gtaattgatt gtccagccat tgggttactt cttgttgaag
      301 agggagaccc aattgcgggc gccgcgcagc cgcgcgagct cctgctggtt gtactcaatc
      361 gacttcattg ccgtgtaagg atccatcgtt gatcccgccc ttctgcgtgc ttccgcatca
      421 ttgccgtttg ccggttcgcc tcgttgcgtt tgattgacgt cgtcgtcaat caccgacatc
      481 tgtatcgtag gcgatggcgg gggctgcgtc accggcgatt gggcggacct tgcacctgat
      541 cttcgggaca ggttttgggg tggttcagca gacagcgcca gtcacagcgg ttgtgatact
      601 tctgtgacgc ggcgatgctc gcaaaacgtg cgtcggttgc tacgatgtga ccatcacgat
      661 cgagtcatcg aatgcgggga tccaggggtg ccggccgatc gccgtcagcg gtgatcgttc
      721 gcggtcgccg cgcctggctc cagaaacgcg tgtcctgtga cgcatgcccg gctcaagcgc
      781 ggtatcatac gcgcctgaag caccgactgc cacaaggaca tgcatgcaca tgagcaattc
      841 ccgttttccc cgcccgatcg accccgatgg tcagcgcaag ctcgccccga ccgacatctc
      901 ccagttcatc cgtctcgatc agtgcgaccg ttacctgcgc ctgcgcctcc acgagcgcgc
      961 cttcggccag cgcttcatgc gtgagtacga tgtgacgccg caatcgattc cggcgctgct
     1021 gacacgctcc ggctccgtct tcgagatgaa gaccgagtca accgtcgcca accatttccc
     1081 gatcgagaac tacgcagccc gcgaccatga atccggctcg cgccaggcag acaacgagcg
     1141 gctgatcggg gccatccgcg atctggagcc ggattcggtc ttcgtcgtgt ttcagccacg
     1201 gttggaagtc gatgtcgacg gctggatgat tcggggtgat gtcgatatcc tgcggctgga
     1261 acgggacgtt gctggccggt tgacgatcac gatcgcagac atgaagagct cgcgatccgc
     1321 gaaggtcgag caccgcgtcc aggtggcctt ctaccacgcg atgctgacgc ggctgctggc
     1381 gactcacgcc atcgagaccg acacgatccg gatggcgatt ctctatcgtg gcccctcgga
     1441 ggccgatgac gcgcagctcg acgacgagga gcgacagcgg cgcgaggcac agcgcgcggc
     1501 ggcacgggag ctgttcggcg tcaccgacgc actgctggag atcgtagagg accccgagca
     1561 ctacctgcgg gccgtcgagg acctggtgac cggaaaggac tcgcgggcca ttcgcgtcgc
     1621 tgaggcgccg ttcgatcatg tgccgtatca cctgacctac aagtgtgatg gctgcatcta
     1681 ctccgagttc tgcatgaagt ggagcgccga gcgggatgat ctctcgctga tcccgcacct
     1741 gacgatccag caaaagagct cgctgcgccg ggcggggatc acaaccacta gccaactggc
     1801 ggcgctgaag caaccggtgg cggatgatcc caacaccctc cagccggcgc cgggacacga
     1861 agcccagacg cgagcgctgg cggcgacctg gccggtcgga ccacggctgg acgagctgat
     1921 ccaccgtgcg cgacgctacc gcaagtacgt caaggacccg atcgaggcca tcagctatct
     1981 gccgagcaag ggctacggct cgctgccata tacggacgcg agccacaatc cgaatctggt
     2041 ccggatctac atcgacgccc agcacgacta cctgaacgat cgcgtctacc tgctcggtgc
     2101 gctgctggtc ggctgcgagg acggcgtcga gtcgccagag cggcggaagt cggtcgtcat
     2161 gatgagcgat ggtccgccgg caacggcaga agatgagcgg cacctgctgg tgggctggat
     2221 cggcggcgtc ctggcgaccc tggaagaggt cgccgcccca aatgaagagg gtttgccgaa
     2281 ggcgccgatc cacctgatct tctacgaccg gatgcaacag acgatgttgc tcgacgccct
     2341 ctcgcgccac ttcgccagca tcgtcgaggc gacgcccctc ttcgatttca tcacccagat
     2401 tgccgccttc gactcgccgg tcgcgacttt cctggaggac gagatacggg agctgaagaa
     2461 ctacccgatg gtctgccagt cattgcagtc ggtcgctgcc tacctgcgct tcgactggaa
     2521 ccagcctgag gaataccgtc ggctgttcag cgtccggctg tttgacttct ggggcaagct
     2581 ggagcaggag aatggcgaga tggcgtggta caccaaccgc gcgcgcttca acagcatgat
     2641 cccgctcgaa tatgcctacg cagcctgggg ggatctcgac gtcccggccg atgccgatcc
     2701 cggcgagtac cggcgcttcg ggggcgtgga cgccacgctg attcagggct tccagcggag
     2761 gcggcttgag gcacttgagc acatcgcgag ggacttcagg gggaacaagc agaccgtcaa
     2821 gagcgcgttc gacatcccga acctggctga gttcgatgac cgctcacggg agatggccaa
     2881 cgcgctgcgc gagttcctga cgatcgagcg ccacaccgag ctgaccgcct ggaagaacgc
     2941 gcgtctcgcc ccaccagagc ggcgggtgct ggccggcgac acactgctgg tgcgttacgt
     3001 cgaggccgat caggagccgg gcgttgccgc gctgaaccgc gacaaccgcc gccggatgga
     3061 gctgcgcgag aagtatcgcg ctgcctacgc ggccgagcat ccggacgcgt cgcggatcac
     3121 gctcccgaag gagcagcggc aggagtccga ctggagccag gacgggattg ggttctggct
     3181 acgggtcgag actgacggcg tcgattgcga tctggaagag attctgagcc tgaccagctt
     3241 ccgcgagcgc gactcgctcg tgttgttccc acgctgggcg gttgactcgc ggttgcccga
     3301 ggacgagcgc tacgagttca ccccgacgcc gaagcagttg ttgtatggca tgcgggtcat
     3361 cctgaagcag atcgtgatcg accggccggc gggaggccgt gcgaagtccg ccagggtgct
     3421 ggtcgagatg cagaacggcc agggccgcgg ccagaacggg ttcgtgttcg gcacgacccc
     3481 gttcaacaat ctgccgctcg aacagggcaa ggtctacacg ctcgattcga acccgaccga
     3541 ctggtacggc tcgcattgcg tcgaggtcgt caaggggctg atcgagggtg gcgttaacac
     3601 cctcttcgac tggctgtccg acacggcagc gctggcaggc gcgcgacacg actggcccct
     3661 tgcggcggcc gaggggcagc gccgcttcct ggccgggctg gacgcgctcg gcgcggctgg
     3721 tgcgttgcac ggcttcgagc cgggcaagcg cgactacatc gccggacacg gcgacgcacc
     3781 gatcctgctc gttcagggac cgcccggcac cggcaagagc tactcgaccg cgttcgcgat
     3841 cttcgcgcgc attcagggtg caatggcagc cggcatcccg ttccgcgtct tcctgacctg
     3901 caaaacgcac gcggcgaccg atgtcctgct cgacaatgtc ctcaatgttc agcgcaagct
     3961 ggccgactgg ttcatcgagc agccggagat cgccgagcag ttctttgacc ggcgcttgac
     4021 acaggtgcca ctcttccgga tccgcccgcg cggagacgtt gccggactga tcgtgccgct
     4081 gctggacgat cgcaacaagc cggcggggaa gaaagcgatc gaccagatca tggcccagga
     4141 gtgggccatc gtcgcggcgg cgcccgccgg aacgcgcagc gcgatcaagg ataagtggag
     4201 caaggagatc ttcggccacg aggttgccga ctgcctgatt ctcgacgagg cgtcgcagat
     4261 gaacctgccc gaggcgatcc tcgcagggct gccgctgaag caggatggtc agttgatcgt
     4321 cgtcggcgac catcggcaga tgccgccgat cgtcaagcat gactggtcca gcgagccacg
     4381 acgaacgttt caggagtacc gttcttacga gtcgctcttc gaggcgttac ttccggtcgg
     4441 cctgccgatc atccgcttcg aggagagctt ccggctgcac gccgagatgg ccgaattcct
     4501 gcgccgcgag atctaccagc acgaccagat cccgtttcac tcgaagcgaa cgacgaccct
     4561 cggtccgatt gcgcataacg acccgttcgt cgcggccgtt ctggacccgg cctacccgct
     4621 ggtcgtcatc gtgcatgacg aagccggctc gcagttgcag aacgactgcg agcgcgagct
     4681 gatcacgccc gttctgcacg cactggcgac gatcccgctc gtgaacgggc acgacaacct
     4741 gccgcgcaac ggtctcgacc cgtacgtcgg actcggtgtt gtcgtcccgc accgcgccca
     4801 gcgggccgcg ttcatcgagc atgttccgtc ggtgacggag cgggacaagg acaccggcac
     4861 gatccgcaag agcgccgtcg acacggtcga gcgcttccag ggcgacgagc ggcaggtgat
     4921 cgtctacacg gcaaccgaga gcgacccgca gtacctcgtt gccgccagca agttcctgat
     4981 ggacccgcgc cggctgacgg tcgcgctcag ccgggcgaag tcgaagatca tcgtcgtcgc
     5041 ctcacgctcg gtcttcagca tcttcagcgc cgacgaggag accttcgcca acgcccagct
     5101 ctggaagaac ctgctgcgtg aaacctgcat cgttcccctc tggagcggcc cgcgtacggc
     5161 gaacggtgtc gaggttggtg tcgaggtctg gggcaaccag ccgggcgtca cccaacaggc
     5221 tctaacgcct tgaggatcac gcgccggaag taggtctccg cgctgccgat gtgggcgagg
     5281 atacggcgca ccagccactc gccgtcgccg gggtcgcggt cgaggatgtc ttcgggtgcg
     5341 ccgatcaggt gaccttcgag gatgccgctt tgtcgccagc agaggccgag caggcgctcg
     5401 gcctccgtct gctgccggcc gcgttgcagc cgccacttga gcgtctggtc cgtgtgctcg
     5461 cgctcgtggt gcgcgaaacg catcagcagg aagcgaacgt cgatcgcgat ctcgccccag
     5521 cgtgcgggcg cgcgtagctc cgcttcggtc aaatcggcga gagcggtcag cgcctcatca
     5581 cggacgtcgt catgatcctg gatcagttgg gccagcgtcg caccgggccg ctcgacctgt
     5641 agttcgtcgt ccggcaggtc gccgtgcggc tctccggcgt ggaattgttt gagcgcgtgg
     5701 tggatgtcca gcgtgtagta gcgctcgacg acgatcaggt gttcgaggat ctgccgcagc
     5761 gcccactcgc cgtccggtag cccctctctg ctatccagat cgtcgttggt caggccggtc
     5821 agcgcggcgt ggaggtcgcc atgggcggtc tggttggctg cccagatcag gtgcgcctgg
     5881 gtccgttcga cgccgaagcg gccacgcagg ataccctcga tctggatcgc gtgctcctca
     5941 aggtgatcgg gacggcgcag gaagatatcg cggacggcgg cgccggtcgt cttgccctgc
     6001 ccgccggtca tcatcgtctc gaaggtgaac gccgtctgaa tgcccatctg tgtctcggtg
     6061 atctgcgcca gttccgcgtc gtgcttgcgc cgcttcttgc gcatctcggc catgatcgtt
     6121 cgaatctcgg acacggaacg caccttcccg atgaacccca ccgtcaaatc agatcacatg
     6181 ttcgcatcgt acgttcagga accacgctcg tcaatgactg ccgcactgct cttcacggaa
     6241 tcggccggct ggtggacaat gccgccagcc gggttgcgtg atgaaaggag cgggtgatgg
     6301 ccagcgcgga tgtggttttc gtcaacgggc ttgtgcggac gctcgatccg gggaatccgg
     6361 ttgccgaggc cgtgctggtc cggggtggcc ggatcgccgc cgtcggcgat ctgaccgagg
     6421 tgcgcgggca agccagcggg gcaagggagg ttgacctggg cggcgcgacg ttgctgccgg
     6481 ggttcgtcga gagtcacaac cacttcatcg acgccggcca ggcgttcgcg caggtcgatt
     6541 gccggggcga gcgggtcggc tcgatcgccg aattgcagca ggcgctgcgt gagcgggctg
     6601 aggtgacacc ggcgggcaac tacgtgctcg ggcgcggcta tgaccagacg ctgctggcgg
     6661 aggatcgtca cccgacgagg gacgatctgg acgccgctgt tcccgatcat cccgtcatca
     6721 tctggcacat ttcctatcac gggctggtgg cgaactcggc ggcattgcgg cgggccggtg
     6781 tcgatcgcaa cacggcggat gtcgatggcg gcgagattct gcgcgacgag cacggcgagc
     6841 cgtccggggt cttcctggag agcccggcga tgtcgctggt gacggcgccg ctcggcgagc
     6901 cgacggtcgc cacgctgcgg gccgggctgg cccatgcccg cgaccagttc gttccgaccg
     6961 gcatctcgac cgtccacgat gcgctgatcg cgacccgtgt caagatcgac gcctacgccg
     7021 aggcgatgct ggccggggag ctgccgatcc gcgtctacat gatgatcgca ccggagctct
     7081 acgctggcgc gctcgaaggg caggccggcg atctggggcc gtcctacggc ttgccggccg
     7141 gtcgcctggt ggccgggccg gtcaagctct ttcaggacgg cgcgatccag gcctacacgg
     7201 cctcgctgac ggagccgtac ttcgacgcgc ccgaccgcta cggcgtcaag atctacgagc
     7261 cggcccatct ggccgagatg gtgctggagc atcacctggc cggtcgccag atcgccattc
     7321 atggcaatgg tgacagcgcg atcgacgaca tcctcaatgc ctatgaggcg gctctggctg
     7381 ccaagccacg cgccgaccac cggcaccgga tcgagcattg ccagacggcc cggcaggacc
     7441 agctcgaacg gatggcgcgg ctcggcgtcg tttccagcgt cttcgccaac cacctctggc
     7501 agttcggcga ccgccacatc gcccgtttcc tcgggccgga gcgggcggca cggatggagc
     7561 cgctggccag tatgaccgag cttggcatcc gctgggggct gcactctgac tgcccggtca
     7621 ccgaggtcaa tccgctgcgt gggatctggc tggccgtcaa ccggcggact tccagcggcc
     7681 agccgatcgg cgagcgcgag cgggtgagcg ccgaacaggc attgcgcggc ttcggcccgg
     7741 acgcggcctt cctcggcttc caggagcagc agctgggcac gatcgcgccc ggctatttcg
     7801 ccgatttcgt tgtcgtctcc ttcgacccgg tcgccgatcc ggaggggatc aaggatctgg
     7861 acgtgctggc gacggtcgtc ggaggtgaga tcgtccatca gtcgccgatc gcgaactgat
     7921 ccggactggc ggtacaatcg tgagcatacg atggtgaggt tgattcgcaa caacgcacga
     7981 tcagatcgag gcagtaccgg ggctggcgtt cgatcgatgg ttgaatccac ctgagggagc
     8041 agcgtttgtc cgacgctggc aacaccgtta cagacgcaga ggcagtcagc cggcaggatg
     8101 gcgaacgcgc ccggatgcag caggcggtgg cgcagctggg acagcgtgcg ctcgtcagca
     8161 gcgatctcga agcattgatg gacgcggtcg tcacgatagt tgcggatgtc ctcgcggttg
     8221 agttctgtaa ggttctcgaa ctgctgccgg acggcaagtc actgttgctg cgggccggta
     8281 ccggctggaa cgaaggagtc gtcgggcagg cgatcatcgg cgttgaggac gactcacagg
     8341 ccggcttcgc gttgatgtcc agccagccgg ttgtcgtgaa cgacctgcgg gtcgagtcgc
     8401 gcttcaacgg gccacggctg ctggtcgagc atggcgtcat cagcgggatg agcgtcatca
     8461 ttcacggcgc gaacgatccg tggggtgtgc tgggcgcgca caccgcccgg ccgaggacgt
     8521 tcaacgacga tgatgtcaac ttcatccagt cggtcgccaa cgtgctggcc gcggctatcg
     8581 accgctcgct ggcggaagac gcgctcagcg agagtgaaga gcgtttccgg acgctggttg
     8641 agatggcgcc ggatgtcatc ttcagtgtcg aagtcaccga tggcactttc acatcgctga
     8701 gtcccgcctt cgagcagttg accggctggt cgcggtcgga atggctgggc cgctcgttca
     8761 ccgagatcgt ccacaccgac gatctgccgc tggcctatca ccatttccaa cgcctgcttg
     8821 ccggtcagct gccgccaccg ttcgagttgc gtgtgacgag ccgagacggc cgcgagatca
     8881 tcggcgagtt ccgggtgacg ccgcaggtcg agggcggcca ggtcgtcgcg ctgttcggga
     8941 tcgcccgcga cgtcagtgac cggcgacggg ccgaggagga attgcgccgc tcgcgagacg
     9001 agctggagat catcctgcgc ggcgttgccg atgggatcac cgtccaggac gcgaccggtg
     9061 cgctcgtcta cgcgaacgag acggcggctc ggctgatcgg ctgcgcgacc gtcgaggagt
     9121 tgctgacaac gcccaccacg gaactactcc gcaactatga gatgttcgac gaggctggag
     9181 cgccgttccc gctggaccga ctgccgggcc ggctggcgtt gctcggccag ccgtcatccg
     9241 aagttctgat gcgctcgcgc gtcatcacga ccggcgagga gcgctggtcg gtgctcagcg
     9301 cgacgccggt tccggggccg gatggcgccc cacgcttcgc gatcaatatc ttccatgacg
     9361 tgaccgagcg catgcggacc gagcgggtcc atgactttct ggcccgcgcg acggcgctgc
     9421 tgtcggcgac gcttgaatac gagcagacgt tggaccgtgt cgctcacatc gcggttccat
     9481 tcctggccga cctgtgcgtc ttcgatatcg tccggccaga cggcagcctg cggaggatcg
     9541 cagcccatga ggatcccgag accgagcggc tgatgcacca gctcatcaac cagcatccga
     9601 tcgagccggg ccatccggtc atgctcgcga tccagaccgg caaggtgacg ctccatgtgg
     9661 atatcgccag tgaagcggtc gctcgcgccg ggttgaccga tgcgcgggtc gaggtcatcg
     9721 ccgcgctggg gttccgctcg gtgatcgtcg tcccgct
//
LOCUS       DASUGJ010000044         6885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1534038, whole genome shotgun sequence.
ACCESSION   DASUGJ010000044 DASUGJ010000000
VERSION     DASUGJ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534038"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFV93_01125"
     CDS             complement(<1..272)
                     /locus_tag="VFV93_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5163968.1"
                     /translation="MNASYDTIVVGLGAMGSATVYQLAKRGQRVLGLEMFQPGHDQGS
                     SHGYHRMIRMSAVFADGYTPLAERALDLWHSLEEESGETLLRLLGE"
     gene            584..1474
                     /locus_tag="VFV93_01130"
     CDS             584..1474
                     /locus_tag="VFV93_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5163969.1"
                     /translation="MSQSVTTTNHPVRFGLRSSQNHRSWAQIREAFVAADEWGLESAW
                     VFDHMIPLSDPRTGPNLEGWTLLAGLAEATKNVMLGTMVTGITYRNPSLLLKEATTVD
                     HISNGRALLGVGAAWFEGEHEMFGFEYPSNGERVSRFREALEMFELLLTQEKTTYDGT
                     YYQLKEAIFEPKPIQKKGGKPHLPVVIGGSGDRMLRIIATHADWWDNNFRDDNEYRER
                     LGKIEAACAKLGRDPKEIRRSTTLGVDFTKGSEKEQRQRLDQLRALGITDFLFHAPPD
                     VTQVRGFMEKVVPELRQAWA"
     gene            complement(1591..2280)
                     /locus_tag="VFV93_01135"
     CDS             complement(1591..2280)
                     /locus_tag="VFV93_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5163970.1"
                     /translation="MLADLHDRVVLVTGGTGSLGRGVVAELKAANATVVSTAHRPSPS
                     PIDGVEIEVADLRDPGEAVPLVERIVARHERIDALVCLVGGFTSGTFIQTDHQTWHEM
                     VELNLNTAVIAARAALPGMVERKYGRIVTVGSRPAIDPTPNTAAYSAAKAAVIAMTRS
                     LGRELRGSGVTANCILPSTIDTPQNRESMPRADPSRWVAPEAIGKVVTFLCSDAAGVI
                     RGAAIPVYGDV"
     gene            2369..2908
                     /locus_tag="VFV93_01140"
     CDS             2369..2908
                     /locus_tag="VFV93_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5163971.1"
                     /translation="MSLENGQTTEQAPLGFTLAAAARKLAKFYAAALAGGPVTPSQLF
                     FLRQLWSEDGLSLVTLRERAQLDATSASWLADQLEKAGLIERRRNAPDRRIVRIWLTG
                     DGRSLREDLSPQIERWEGSLRETLAIHHRPDEIDAFERVLKTLIVTLPEGDDLWAELS
                     AAWDTTLDALRHYLEAEEH"
     gene            2952..3392
                     /locus_tag="VFV93_01145"
     CDS             2952..3392
                     /locus_tag="VFV93_01145"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5163972.1"
                     /translation="MSTMSLVGRTVEKELFIAAAPERVYRALTNVDDLKRWFVTDAEV
                     ELQPGGVFNLYWQQENVKGEIVESDPPHRLTFTWDERPAHGITTCTIELTPEADGTLL
                     RLAHTGFLEGDNWDDLYNGVDSGWTLELGNLKTWLETGTVKHWS"
     gene            complement(3487..4275)
                     /locus_tag="VFV93_01150"
     CDS             complement(3487..4275)
                     /locus_tag="VFV93_01150"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX5163973.1"
                     /translation="MREEPRRVGLIGFGAIGQPVVEMAARYPDEIDIIAACTRTGVPR
                     VDGAAIPVVGTLDELLRLRPDVVVEIAGHDALREHGPHVLTAGIDLIAVSVGVLAVPE
                     VEQDLLAAAREGGSQLRVASGAIGALDALASAAIGGVERVTHTTRKPPRTLLGDEGAG
                     LTAEREVFHGPAREAALRFPESVNVTAAVSLAGIGFDRTLVRVIADPAVDRNTHQVEA
                     EGDFGYLRFEIRNVPSDDNPKTGRIVAMSIISTLRQPGLPLSIG"
     gene            4638..5708
                     /locus_tag="VFV93_01155"
     CDS             4638..5708
                     /locus_tag="VFV93_01155"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX5163974.1"
                     /translation="MTRVSCAVIPGDGIGREVVAEGVKLLEAAAEMDGGLSFDFEWFP
                     WSCQYYAEHGEMMPVNGLEILGGYENIFLGAVGFPGVPDHLSLWGLLIPIRRTFQQYI
                     NLRPAKLLAGVESPLRDKAPGSIDFVVVRENSEGEYSNMGGRIHSGTPYEMAIQETVF
                     TRMGTERVIRYAYELASKGSKHLTAATKSNGINITMPYWDEIVGEVGAGFPDVRTDLF
                     HIDALAAYFVTRPESFDVVVGSNLFGDILSDLAAAVMGGIGMAPAANLNPERKFPSMF
                     EPVHGSAPDIAGRGIANPIGQIWTAKMLLDHLGKEELGAALLDSIEAVLAAGIVQTPD
                     LGGRATTSEMGDAILAEFRSRG"
     gene            5803..>6885
                     /locus_tag="VFV93_01160"
     CDS             5803..>6885
                     /locus_tag="VFV93_01160"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5163975.1"
                     /translation="MKRSGRVDGALRVVVVIASLLGLMLAAGVRGTPVVETARAADVI
                     TIRRFETDEKVYALTFDAGSDRGYAVKILDTLKANGIHATFGMTGVWAQANPDLLQRM
                     VTEGHQLLNHSWDHPSFNTLTTAQRTDQLKRTEDIVRQTTGVELRPWFRPPYGDYNDS
                     VLSDLSADGYTYNVLWTTDSLGWNGLDAAGIIQRVVDGAVPGGIGLFHVGAASQDAEA
                     LQGIIDRLRQRGYRFATIAELAGTGERYFPETGHTVAGNFLKYWNQFGGLELFGYPIS
                     DAFEWNGMTVQYFERVRMEHQPGMWPARYDILLGRLGSELTADRVNEQPFQRVTGSAN
                     ANCDYYAETDHYLCYGFRDYWRKHGGL"
BASE COUNT         1248 a         2230 c         2273 g         1134 t
ORIGIN      
        1 atctcaccca gcagccgcag caacgtctca cccgactcct cttcgagcga gtgccagaga
       61 tcgagcgccc gttccgcaag cggcgtgtaa ccgtcggcga agacggccga catccggatc
      121 atgcggtggt agccgtggga cgatccctga tcgtgaccgg gctggaacat ctccagaccc
      181 agcacgcgct ggccccgttt ggcaagctgg tagaccgtcg cgctccccat cgcgcccaag
      241 ccaaccacaa tcgtgtcgta cgaagcattc atggtcgtca ttctttcgac tacgcgagcg
      301 ttcggctgcg agggcgatga tacccggact gcgtaagccc gcaaaacccc tcccccaccc
      361 ccctccccta tgcggagagg gggcttttgg ccgcacggat ctaaagcccc ctctccacac
      421 agggccgggt accctctggg tggcggttgg gggaggggtt ttgcgggctc acacgcttcg
      481 tgaagggctt ttcgccaccc acccactgga cggaaccccc gtcctctgtc acagttgcag
      541 ccggccgcca agccgccatc gctcggagtg aaatgaggga acgatgagcc agtctgtgac
      601 gaccacgaac catccggtgc gcttcggcct gcgctccagc cagaatcacc gcagctgggc
      661 gcagatccgc gaggcgttcg tggccgccga cgagtggggg ctggaatcgg cctgggtctt
      721 cgaccacatg atcccgctct ccgatccacg gaccgggccg aacctggagg gctggacgct
      781 gctggccggg ctggccgagg cgacgaaaaa cgtcatgctc gggacgatgg tgaccggcat
      841 cacctaccgc aacccgtcgc tgctgctcaa ggaggcgacg acggtcgacc acatctcgaa
      901 cggccgcgcc ctgctgggtg tcggcgccgc ctggttcgag ggcgagcacg agatgttcgg
      961 tttcgagtat cccagcaacg gcgagcgggt cagccgcttc cgcgaggcgc tggagatgtt
     1021 cgaactgctg ctgacacagg agaagaccac ctacgacggc acctactacc agctcaagga
     1081 agcgatcttc gagccgaagc cgatccagaa gaagggcggc aagccacacc tgccggtcgt
     1141 catcggcggc tccggcgacc ggatgctacg cattatcgcc acgcacgccg actggtggga
     1201 caacaacttc cgcgacgaca acgagtaccg cgagcgactc ggcaagatcg aagctgcctg
     1261 cgccaagctc ggccgcgacc cgaaagagat ccgccgctca acgacgttgg gcgtcgactt
     1321 caccaagggc agcgagaagg agcagcgcca gcgactcgac caactccgcg cactcggcat
     1381 caccgacttc ctcttccacg ccccgcccga tgtcactcag gtccgcggct tcatggagaa
     1441 ggtcgtgccg gagttgcggc aggcctgggc ctagaagggg agggaatcgg gattcggggt
     1501 tcgggagtac gtgagtgccg atgtcgttgg tgacacggtt ctcggtccag actcgattcc
     1561 cgattcccga ctcccgaatc ccgcccctcg tcacacatcc ccatagaccg ggatcgccgc
     1621 gccgcggatg acgccggcgg cgtcggagca gaggaacgtg acgaccttgc caatcgcttc
     1681 gggagcgacc cagcgggacg ggtcggcgcg cggcatcgac tcacggttct ggggtgtgtc
     1741 gatcgtgctg ggcaggatgc agttggcggt gacgccgctg ccgcgcagct cacggcccag
     1801 cgagcgggtc atcgcgatga cggccgcctt ggcggctgag taagcggctg tgttgggggt
     1861 cgggtcgatg gcgggccgcg agccgaccgt cacgatccgg ccatatttgc gctcgaccat
     1921 gccgggcagg gccgcccggg cggcgatgac agccgtattg aggttcagct cgaccatctc
     1981 atgccaggtc tggtggtccg tctggatgaa cgtcccgctg gtaaagccac ccaccaggca
     2041 gaccagcgcg tcgatccgct cgtggcgggc aacgatccgc tcgacgagcg ggacagcctc
     2101 gcccggatcg cgcagatcgg ccacctcgat ctcgacgccg tcaatcggcg acgggctggg
     2161 ccggtgcgcc gtcgagacaa cggtcgcatt ggccgctttc aactcggcga cgacgccgcg
     2221 accgaggctg ccggtgcctc cagtgacgag gacaacacgg tcatgcaggt ctgccagcat
     2281 ggctactccc tttcagtaca gatgatcgaa aacgtggttg acattgatac tctttctaat
     2341 tagaatgttt ccaacaggag gatggtcgat gtcgctggaa aacggtcaga caacagaaca
     2401 ggcgccgctc gggttcacgc tcgcggcggc ggcccgcaag ctggccaaat tctacgcggc
     2461 agcgctggcc ggtgggccgg tcacgccatc gcaactgttc ttcctgcgcc agctctggag
     2521 cgaggatggc ctctcgctgg tcacgctgcg cgagcgcgcc cagcttgatg cgacctcggc
     2581 cagctggctc gccgaccagc tcgaaaaggc cggcctgatc gagcggcggc gcaacgcccc
     2641 cgaccggcgg atcgtcagaa tctggttgac cggcgacggc cggtcgctac gtgaggacct
     2701 cagcccacag atcgagcgtt gggaaggatc gctgcgcgag acgctggcga tccaccatcg
     2761 tcccgacgag atcgacgcct tcgagcgcgt gctgaaaacg ctgatcgtga cgctcccgga
     2821 aggcgatgac ctctgggccg agttgtccgc cgcctgggac accaccctcg acgctctgcg
     2881 gcactatctg gaggccgaag agcactgatt cgcgcttcac tgaacaggac gaaacctgag
     2941 aggagaccgg catgtccacc atgtcgctgg taggccgcac cgttgagaag gaactgttca
     3001 tcgccgccgc gccggagcgt gtctaccgcg cgctcaccaa cgtggacgat ctcaagcgct
     3061 ggttcgtgac tgacgccgag gtcgagttgc agccaggggg cgtcttcaac ctctactggc
     3121 aacaggagaa cgtcaagggc gagatcgtcg agagcgatcc gccgcaccgg ctgaccttca
     3181 cctgggacga gcgcccggca cacggcatca ccacctgcac gatcgagctg actcccgaag
     3241 cggacggcac gctgctgcgc ctcgcccaca ccggcttcct ggagggcgac aactgggacg
     3301 acctctacaa cggcgttgac tccggctgga ccttggaact cggcaatctc aagacatggc
     3361 ttgagaccgg taccgtgaag cactggtcgt aatcgcctga aatcgggatt cggggcacgg
     3421 gattcggggg aaggacgcag tgtttctctc ctccgaatcc cgtgtcccga atcccacgtc
     3481 ccgaactcac ccaatcgaca acggcaaccc cggttggcgg agcgtgctga tgatgctcat
     3541 cgcgacgatt cggccggtct tggggttgtc gtcggagggg acgttgcgta tctcgaagcg
     3601 caggtagccg aagtcgccct ccgcctcgac ctggtgggtg ttgcggtcga cggccgggtc
     3661 ggcgatgacg cggacaagcg tccggtcgaa gccgatgcca gccaggctga cggcggcggt
     3721 gacattgaca ctctccggga aacgcagggc tgcctcgcgg gccggcccgt ggaagacctc
     3781 gcgctcggcg gtcaacccgg cgccctcgtc gccgaggagc gtgcggggcg gcttgcgggt
     3841 ggtgtgcgtc acccgctcga ccccaccgat cgcagctgac gcgagggcat ccagcgcgcc
     3901 gattgcgccc gatgcgaccc ggagctggct gccaccctcc cgtgctgctg ccagcagatc
     3961 ctgctcgacc tccggcacgg ccagaacgcc gacgctgacg gcgatcaggt cgatcccggc
     4021 cgtcagcacg tgcggcccgt gctcgcggag cgcgtcgtgg ccggcgatct ccacgacgac
     4081 atcgggtctg agcctgagca gttcgtccag cgttccaacg accgggatcg cggcgccatc
     4141 gacgcgcggg acgccggtcc gggtgcaggc ggcgatgatg tcgatctcgt ccggataacg
     4201 cgctgccatt tcgaccaccg gctggccgat cgccccaaac ccaatcagcc cgacacgccg
     4261 tggttcctcg cgcatggttc ctgctcccct cattcacctt ctgcattgcc attgtacggc
     4321 caaaatggtt ctttccagac gcatgagcct attgagggac gacacccctt gccctgtcat
     4381 cctgagccgc agcgaaggat cgcctggcgt aggagtgacg agcgagccgg ctatgacgac
     4441 tcgatctgcc attcggcgcg ctcctgtaag gcacgttgag cgatccttcg ctgcggctca
     4501 ggatgacagg gcaaggggca ctgttactcg tcagatagtc gtctctgaga cgtgtcgctt
     4561 cggcaatcga cgggtcacgc cgatgcggat aggatagggg cgcctgaaat gaagacgagc
     4621 gcatgagggg tggagagatg acacgggtca gttgcgcggt gattccaggg gacgggatcg
     4681 ggcgggaggt ggttgccgag ggggtgaagc tgctagaggc ggcggcggag atggacggag
     4741 ggttgtcgtt cgacttcgag tggttcccgt ggagctgcca gtactacgcc gagcacggcg
     4801 agatgatgcc ggtcaacggg ctggagattc tcggcggcta cgagaacatc ttcctcggcg
     4861 cggttggctt ccccggcgtg ccggaccatc tctcgctctg ggggctgctg atcccgatcc
     4921 ggcgcacatt ccagcagtac atcaacctgc ggccggccaa actgctggcc ggtgtggagt
     4981 ccccgctgcg cgataaggcg ccagggtcga tcgacttcgt cgtcgtccgc gagaactccg
     5041 agggggagta ctcgaacatg ggtgggcgca tccacagcgg gacaccgtat gagatggcga
     5101 tccaggagac ggtcttcacc cgaatgggaa ccgagcgggt catccgctac gcctatgaac
     5161 tggccagcaa gggcagcaag catctgacgg cggcgaccaa atcgaacggg atcaacatca
     5221 cgatgccgta ctgggacgag atcgtcggcg aggtcggcgc gggcttcccc gatgtacgga
     5281 ccgacctgtt ccacatcgac gcgctggcgg cctacttcgt gacccggccg gagtcgttcg
     5341 atgtcgtggt tggctccaac ctgttcggcg acatcctctc tgacctggcg gcagccgtca
     5401 tgggcgggat cgggatggcg ccagccgcca acctgaaccc ggagcggaag ttcccgtcga
     5461 tgttcgagcc ggtccatggc tcggcgcccg acatcgccgg ccggggcatc gccaacccga
     5521 tcggccagat ctggacggcc aagatgctgc tcgaccacct cggcaaagag gaactcggcg
     5581 cggcgctgct cgatagcatc gaagccgttc tggcggccgg gatcgtccag acaccggacc
     5641 tcggcgggcg agcgacgacc agcgagatgg gcgacgcgat cctggccgag ttccgctcaa
     5701 ggggataggt cgcaaccgtc accaattggc ggtcccgcgc gttgttacga ggtaacggga
     5761 cctcaaattc ggcatgttcg acatgaaagc tggagcggca tgatgaagcg ctcgggtcgc
     5821 gtggacggcg cgttgcgggt cgttgtggtt attgccagcc tgctcgggtt gatgctggcg
     5881 gccggagtgc gtggaacacc ggtcgtggag acggcccggg cggcggatgt catcaccatc
     5941 cgccggttcg agacggacga gaaggtctac gcgctgacct tcgacgccgg ctccgatcga
     6001 ggctatgcgg tcaagattct cgacacactc aaggcgaacg gcatccacgc cacgttcggg
     6061 atgaccggtg tctgggcgca ggccaacccg gatctgctcc agcggatggt caccgaggga
     6121 catcagctac tcaaccactc gtgggaccat ccgtcgttta acacgttgac gactgcccag
     6181 cgcaccgacc agctcaagcg gaccgaggac atcgtccgcc agacaaccgg cgtcgagcta
     6241 cggccgtggt tccggccgcc gtacggcgac tacaacgaca gcgtcctgag cgacctctcc
     6301 gccgacgggt acacctacaa cgtcctctgg acgaccgatt cgctcggctg gaacgggctg
     6361 gacgcagccg ggatcatcca gcgcgtcgtc gatggggctg taccgggcgg gatcgggctc
     6421 ttccatgtcg gcgcagcctc gcaggacgcc gaagcgttgc aagggatcat cgaccggctg
     6481 cgccagcgcg gctaccgctt cgcgacgatc gccgagctgg cgggcaccgg cgagcgctac
     6541 ttcccggaaa caggtcacac cgtcgccggc aacttcctca agtactggaa ccagttcggc
     6601 gggctggagc tgttcggcta cccgatctcc gacgcgttcg agtggaacgg gatgacggtc
     6661 cagtacttcg agcgggtgcg gatggagcac cagccgggaa tgtggccagc gcgctacgat
     6721 atcctgcttg gccggctcgg atcggagctg acggctgatc gggtgaatga gcagccgttc
     6781 caacgcgtta ccggcagcgc caacgccaac tgcgactact acgccgagac ggaccattac
     6841 ctatgctacg gcttccgcga ctactggcgc aagcacggcg ggctg
//
LOCUS       DASUGJ010000045         4659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1395045, whole genome shotgun sequence.
ACCESSION   DASUGJ010000045 DASUGJ010000000
VERSION     DASUGJ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395045"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..2193
                     /locus_tag="VFV93_01165"
     CDS             238..2193
                     /locus_tag="VFV93_01165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5163976.1"
                     /translation="MPQPLSPETLVYGFTAADDPQVAPDGNAILYTLATMDKESKKGT
                     ANVWICDIDGGNARQLTWGGKRNAGARWSPDGSQIAFISDRAGEKKSGIYVMPAGSGG
                     DARELTTHNQGVSDLVWSPDGAHLASTTLFDPENPDEEEAPKDAAPKVRVTRRIDYKQ
                     DGRGYLNDKRLQTWIVAVESGERRRLTSEPADHAFPRWSPDGGTIAVQVANRNGMCSQ
                     LALIDVATGGEKRISSEMGTISTFSWSPSGDRIIFSGDVRQTFQSDYFIYTVATGETK
                     RLTDDLQQLPVGGAPGDASPSQPIWLDDRQVLFHAVRAGGSGLYVIDSEKGSVEPVQT
                     WQAQHGGFSTDRNKRYIVQSRTSMTEIGNLHVFDNETNEGEVITNYNEDVLAEHPPAQ
                     WERFDIERGGYTIEAWLLKPVDFDASKKYPAILEIHGGPNGFYGYAFNTIHQLLATNG
                     FITVYSNPRGSTSYGRDFTQQVTLDWGGEDYLDLMAVMDKTLELPYVDAERTGVCGYS
                     YGGYMTSWIIGQTDRFAACVCGAPCFDLESMYGTSDISHTFGELQWGGQPHDSEAWYK
                     AHSPSTYAHNAKTPTLIIHGEADERCPIGQGEQMFVALLKAGCEVEFARYPGGSHGFR
                     RNGPPEHRQDCWERTLGWFKDHLGEAK"
     gene            complement(2286..3236)
                     /locus_tag="VFV93_01170"
     CDS             complement(2286..3236)
                     /locus_tag="VFV93_01170"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEX5163977.1"
                     /translation="MTRSRFAGWNLVFLGVMALGFAAAIYHATSGRWGNAIMAAGASA
                     MGTVVILLMPQQMAVAEAAPRRGPRQAVSDMMSRERVEPERDRWLSGSILAGFAATII
                     MSLLLVIAYTLTGYLGDADGSQLSQWFWNLRNNDLATGALDIPITAFSINLLAGLAWA
                     LVYGGIVEPRLSGPGWLRGMIFSLVPWLLSLIVFFPAIGGGFFGMDLEAGPLPAIGNL
                     VLHLIYGAILGAVFAIPEVSSNPLDERAARSQNNGTAIGLVAGLTVGIAIGATIAALA
                     ASDVTDGINITLAGGGIGILLGGLIGSFAGVDVGTRHEMG"
     gene            3563..>4659
                     /locus_tag="VFV93_01175"
     CDS             3563..>4659
                     /locus_tag="VFV93_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX5163978.1"
                     /translation="MSERIRGIIGAGSAVLSKQEDELRERAVGLLARTEAVARAFPGS
                     DRADEELRLLRQAKQQLETLFLLVIVGEFNAGKSAFINALLGEPVMPEGVTPTTSMIH
                     LLVYGEAGQEDVTSDGVIVHRHPAPFLREINVVDTPGTNAIMREHEAISQRFVPRSDL
                     VLFVTSADRPFTESERAFLEEIRNWGKKIVVVLNKIDLLESDEQLQEVVSFISGNMAR
                     LLGIEPLIFPIAAKLAERGDPRGRFDALREYIITTLDEEERIRLKLLNPLGVAERLLA
                     SYSGIADERLSLLEQDAATIARIEALLTDYEAEMRREFATYITRVENIVHRLNERADR
                     FFDEYIRIGRVFDLMRSDNTRDAFERDVVADT"
BASE COUNT          866 a         1501 c         1555 g          737 t
ORIGIN      
        1 cagccgccgt tgtcgcatgg ttcgatcctg tcatgtgccc tgccttcccg gcgggttccc
       61 tcccgcgtgc gcgttccagc gcgggaacgc ggctcgattg ctgattagag taggcgagga
      121 ttggccgatc gtcaatcacc catcgggtca tttgagtcgt tgccaccggc gcacgatgac
      181 ggtattctcg cgcggcggag gtcaaccgcc cgcggaggga tggagaagga gttcgagatg
      241 ccacagccac tttcgcccga gacactcgtc tacgggttta ccgccgccga cgacccgcag
      301 gttgcgccgg atggcaacgc gattctgtac acgctggcga cgatggataa ggagtcgaag
      361 aaggggaccg cgaacgtctg gatctgcgac atcgacggcg gcaacgcgcg ccaactgacc
      421 tggggcggca agcgcaacgc cggcgctcgc tggtcgccgg atggcagcca gatcgcgttc
      481 atctccgacc gggccggcga gaagaagagc ggcatctacg tcatgccggc cggcagcggc
      541 ggcgacgcgc gcgagctgac gacgcacaac cagggtgtca gcgatctggt ctggtcgccg
      601 gacggcgccc atctcgcctc cacgacgctc ttcgaccccg agaaccccga cgaggaagag
      661 gctcccaagg acgccgcgcc gaaggtgcgc gtcacccgcc ggatcgacta caagcaggac
      721 gggcgcggct acctgaacga caagcggctc cagacctgga tcgtcgccgt cgagtcaggc
      781 gagcgacggc ggctgaccag cgagccggcc gaccacgcct tcccgcgctg gtcgccggat
      841 ggtgggacga tcgccgtcca ggtcgccaat cgcaacggga tgtgctcgca gctggcattg
      901 atcgatgtcg cgaccggtgg cgagaagcgg atcagctcgg agatgggcac gatctcgacc
      961 ttctcgtggt ctccctccgg cgaccggatc atcttcagcg gcgacgtccg gcagacgttc
     1021 cagtccgact acttcatcta caccgtcgcg accggcgaga cgaagcggct gaccgatgac
     1081 ctgcagcagt tgccggtcgg tggtgcgccg ggcgatgcct cgccgtcgca gccgatctgg
     1141 ctggacgacc ggcaggtgct cttccacgcg gttcgggctg gtggcagcgg gctgtatgtg
     1201 atcgacagcg agaagggcag cgtcgagccg gtccagacct ggcaggcgca gcacggcgga
     1261 ttcagcaccg accgcaacaa gcgctacatc gtccagtcac ggacgagcat gactgagatc
     1321 ggcaacctgc acgtcttcga caacgagacg aacgaaggcg aggtcatcac caactacaac
     1381 gaggatgtgc tggccgagca cccgccggcc cagtgggagc gcttcgacat cgagcgtggt
     1441 ggctacacga tcgaggcctg gctgctgaag ccggtcgact tcgacgccag caagaagtac
     1501 ccggccatcc tggagatcca cggcgggccg aacggcttct acggctacgc cttcaacacg
     1561 atccaccagc tactggcgac gaatggcttc atcaccgtct actccaaccc gcgcggctcg
     1621 acgtcgtacg ggcgcgactt cacccagcag gtgacgctcg actggggcgg cgaggactac
     1681 ctcgacctga tggcggtcat ggacaagacg ctggagctac cttacgttga cgccgagcgg
     1741 accggcgtct gcggctactc gtacggcggc tacatgacgt cgtggatcat cggccagacg
     1801 gatagattcg ctgcctgtgt ctgcggcgca ccctgcttcg acctggagtc gatgtatggc
     1861 acgtccgaca tcagccacac gttcggcgag ctccagtggg gcggccagcc gcacgactcc
     1921 gaagcgtggt acaaggcaca ctcgccctcg acctatgccc acaacgcgaa gacaccgacg
     1981 ctgatcatcc acggcgaggc cgacgagcgc tgcccgatcg gtcagggcga gcagatgttc
     2041 gtggcgctgc tgaaggccgg ctgcgaggtc gagttcgccc gctaccccgg cggcagccac
     2101 ggcttccgcc gcaacggccc gcccgaacac cgccaggact gctgggaacg gacactgggc
     2161 tggttcaagg accatctggg ggaggcgaag tagcccccgg cgatcaatcc aataatccaa
     2221 tccggtagag gcgacactgc gatgattgcc cctaccggat tgggattgac atcatcgaat
     2281 tgacgttacc ccatctcgtg ccgtgtcccg acatcgacgc cggcgaagga gccgatcagg
     2341 ccgccgagca ggatgccgat gccgccgccg gcgagggtga tgttgatgcc gtcggtgaca
     2401 tccgaggcgg ccagggcggc gatggttgcg ccgatggcga ttccaaccgt caggccggcg
     2461 acgagaccga tcgcggttcc gttgttctgc gatctggccg cccgctcgtc gagcggattg
     2521 ctgctgactt cagggatggc gaagacggca ccgaggatgg ctccatagat caggtgcagg
     2581 acgagattgc cgatcgcggg cagcggcccg gcctcgaggt ccatcccgaa gaagccgccg
     2641 ccgatcgccg ggaagaagac gatcagtgag agcagccacg ggacgagcga gaagatcatg
     2701 ccgcgcagcc agccggggcc ggacaggcgt ggctcgacga tgccgccgta gacgagcgcc
     2761 caggcgagac cggccagcag gttgatgctg aaggcggtga tcgggatgtc gagcgcgccg
     2821 gtcgccagat cgttgttgcg caggttccag aaccactgcg acagctgact gccgtccgcg
     2881 tcgcccagat agccggtcag ggtataggcg atgacgagca gcagcgacat gatgatcgtc
     2941 gcggcgaagc cggccaggat cgagccgctc agccagcgat cgcgttccgg ctcgacccgc
     3001 tcgcgcgaca tcatgtcgct gacggcctgg cgcggacctc gccgcggcgc agcctcggca
     3061 accgccatct gttgaggcat caacaggatc acgactgttc ccatcgcgct ggcgccggcc
     3121 gccatgatcg cgttgcccca ccgcccactg gtcgcatggt agatcgccgc agcaaagccc
     3181 agcgccatga cgcccaggaa gaccaggttc cagcctgcga atcgtgatcg tgtcaagggg
     3241 agcctccatt cctcgcggtg gtcgcaaccg aggggcggtg cggccggagt cattgacctc
     3301 ataccatagt catctacgcg atttcgtcaa gagtcggttt acgatcacgc aattgtgatg
     3361 gttatcacaa acatcaccac gatagatgat ctatcgggtt gtcggttcac tcgtcgctgt
     3421 acgatgtcaa cgcccgctac tggagatgtt gcagaaggtg tgccaacggc tgaacgtcgg
     3481 cgtttccgac ccggcactcc cggcaggcgg gttgcaatgg caaaatcagg gtgcggctgt
     3541 cgcgtatcac agaggagacg ggatgtccga acggatcagg ggcatcatcg gagccgggag
     3601 cgcggtcctg tcgaagcagg aggacgagct gcgcgagcgg gcggtcggcc tgctggcgcg
     3661 gaccgaggcc gtcgcacggg ccttccccgg ttccgaccgg gccgatgagg agctacggct
     3721 gctgcgccag gcgaagcagc agctggagac gctcttcctg ctggtgatcg tcggcgagtt
     3781 caacgctggc aagtcggcct tcatcaacgc actgctgggt gagccggtca tgccggaggg
     3841 cgtcacgccg acgaccagca tgatccacct gctggtctat ggtgaggcgg gtcaggagga
     3901 tgtcaccagc gacggtgtca tcgtccaccg ccacccggcg cccttcctgc gcgagatcaa
     3961 cgtcgttgac acgcccggca cgaacgcgat catgcgcgag cacgaggcga tttcgcagcg
     4021 cttcgtgccg aggtcggacc tggtcctgtt cgtgacctct gcggaccggc cgttcaccga
     4081 gtcggagcgg gcgttcctcg aagagatccg caactggggc aagaagatcg tcgtcgtcct
     4141 caacaagatc gatctgctgg agagcgacga gcagctccag gaggtggtca gcttcatctc
     4201 cggtaacatg gcgcggctgc tggggatcga gccgctgatc ttcccgatcg cggccaagct
     4261 ggccgaacgg ggcgacccac gcgggcggtt cgacgcgctg cgcgagtaca tcatcacgac
     4321 gctcgacgag gaggagcgga ttcggctcaa gctgctcaac ccgctcggcg tcgcggagcg
     4381 gctgctggcc agctactccg ggatcgccga cgagcggctc tcgctgctgg agcaggacgc
     4441 ggcgacgatc gcccggatcg aggcgctcct gaccgactac gaggcggaga tgcggcgcga
     4501 gttcgcgacc tacatcaccc gggtcgagaa catcgtccac cgtctcaacg agcgtgccga
     4561 ccgcttcttc gacgagtaca tccgcatcgg acgcgtcttc gacctgatgc gctccgacaa
     4621 cacgcgggac gccttcgagc gcgatgtcgt cgccgacac
//
LOCUS       DASUGJ010000046         5235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1256144, whole genome shotgun sequence.
ACCESSION   DASUGJ010000046 DASUGJ010000000
VERSION     DASUGJ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256144"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1630)
                     /locus_tag="VFV93_01180"
     CDS             complement(173..1630)
                     /locus_tag="VFV93_01180"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5163979.1"
                     /translation="MVAETQTRSFQLLIAGKAVDAASGETFTAYNPATNQPVAQVAKA
                     GKEDVDRAVAAARKAFDEGPWPRMSPYERGRIVQKLADRIRERADEIAALESLNTGKP
                     ITRSKGEILGSAMVFDYYAGSGDKFYGETIPMGDKVLDFTLREPVGVVGQIVPWNFPF
                     SMASWKVAPALASGCCLILKPASNTPLSAILLGEICYEAGVPEGVVSVLPGPGGEIGE
                     YLAAHPQVDKIAFTGETITGARILKASADTIKKVSLELGGKSPNIVFADADLKKAAAA
                     AVPAAFGNTGQTCTARTRLFVGQSSYDNFMQELIDQTESYKVGDPFDTATLMGPMIST
                     AQYDRVTSYIDIGKGEGAELVFGGARPSALDEGNFLQPTIFGQVSNDMRIAREEIFGP
                     VLSVIPFADEEDVIRQSNANDYGLAASVWTQDINKALRVAKGLRTGMVAVNSNGGPGV
                     LGPFGGYKKSGLGRELSMHGMELYTEVKNVYIDLS"
     gene            complement(2070..2675)
                     /gene="dcd"
                     /locus_tag="VFV93_01185"
     CDS             complement(2070..2675)
                     /gene="dcd"
                     /locus_tag="VFV93_01185"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX5163980.1"
                     /translation="MILSDRDIRREIENGRIRITPEIDLDKHMGSCSVDFRLGNTFMV
                     FEHSRYSYIDPRQPQSIGEGMRTIVVKDDEPFIMQAGDFALASTIESLELPDDLLGRL
                     EGRSSIARLGITVHSTAAVFEPGWIGTATMELSNLGRMAVALYPGMRICSFTFQQLSS
                     PVLVPYRQKAGNKYAGQNEPRASLLSEEQRALTESSNGRLS"
     gene            3298..5169
                     /locus_tag="VFV93_01190"
     CDS             3298..5169
                     /locus_tag="VFV93_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX5163981.1"
                     /translation="MPVVSPEPGVPATSELVASPPETESDATDDPVTEAEATEADDAP
                     TKIAEADEPPAEPVAAAQPDDQAEAGESDYADYARSVEEAAARSRLEMAPQAAATAAY
                     PAIRPAAASGAAVQRPTVRQTRGRPVVGSRVRSGGGGNGNGGRRRTANGGHERDPRPL
                     LKRPKTWLVITGIVVAVVAIAAVLWAANIARITYNAYNEAHVDTGERDIYTVNAEGTP
                     VIVPTEQVQASLPNWDNKDPFNVLLMGIDDREGDTEPPRSDTMIVMRINPANNSVVMM
                     SIPRDLLVYIPVADRWDKINAAYPIGEEKDENGGPPAVMETIEANFDVRIHYFVTVDF
                     NGFRSIVDTVGGVVIDVPAPVKDDQYPTEDFGITREYFSTGLQKMDGETALRYSRTRH
                     NDNDIARGERQQQMLIALREQAIDLGLVTKAEELISQMGSMVKTDFNFNQLLALANLG
                     RKIDPNAISKVNLWDAGVLSEHAPEDELDPFYFEADWPAVYKLMDQYFNSDGNAPPPP
                     TPTVSVPDEPLASPRTGGSNVQFDTPIIVQNNAGQTALARAVGQIMTDTGFTVVAPET
                     GNGEQTSTIIYDYAERPDTARYIAEQLGLDESSIVYGNGGNGIVVSLGSDLAWILDQ"
BASE COUNT          977 a         1760 c         1651 g          847 t
ORIGIN      
        1 ggtcaaagcg gagaggtcct tcgctgcggc tcaggacgac agacggagat ctctggcccg
       61 tctcacacta ttcgctgtgg atggctcgaa cgtcacgcaa cccgaaatca caatggccgg
      121 agtggataca ccccggccat ctgactcaac actggcgtct gagcgccaga ggttagctca
      181 ggtcgatgta gacgttcttg acttcggtgt agagctccat gccgtgcatc gagagctcgc
      241 ggccaaggcc ggacttcttg tagccgccga acgggccgag cacgcccggg ccgccgttcg
      301 agttgacggc aaccatccct gtccgcaggc ccttcgcgac gcgcagcgcc ttgttgatgt
      361 cctgggtcca gaccgatgca gccagcccgt agtcgttggc gttcgactgg cggatgacat
      421 cttcctcgtc cgcgaacggg atgaccgaga ggaccgggcc gaagatctcc tcgcgggcga
      481 tccgcatgtc gttggagacc tggccgaaga tcgtcggctg gaggaagttg ccctcatcca
      541 gcgccgacgg ccgcgcgccg ccgaagacga gctcggcgcc ctcgcccttg ccgatgtcga
      601 tgtaggaggt gacgcggtcg tactgcgcgg tcgagatcat cggacccatc agcgtcgccg
      661 tgtcgaacgg atcgccgacc ttgtacgact cggtctggtc gatcagctcc tgcatgaagt
      721 tgtcgtacga cgactggcca acgaacagcc gggtgcgggc cgtgcaagtc tggccggtgt
      781 taccgaaggc agccgggacg gcagcggcgg cggccttctt cagatcagcg tcggcgaaga
      841 cgatgttggg gctcttgccg cccagctcca acgagacctt cttgatcgtg tcggccgagg
      901 ccttcaggat ccgcgcaccg gtgatcgtct cgccggtgaa agcgatcttg tcgacctgcg
      961 ggtgggcggc caggtactcg ccgatctcgc cacctggccc cggcaggaca ctgacgacgc
     1021 cttccggcac accggcctcg tagcagatct cacccagcag gatcgctgag agcggcgtgt
     1081 tgctggccgg cttgaggatc aggcagcagc cggaagcgag cgccggcgcg actttccatg
     1141 aggccatcga gaatgggaag ttccacggca cgatctgacc gacgacaccg accggctcgc
     1201 gcagcgtgaa gtcgaggacc ttgtcgccca tcgggatcgt ctcgccgtag aacttgtcgc
     1261 cggacccggc gtagtagtcg aagaccatcg cactgccgag gatctcaccc ttcgagcggg
     1321 taatcggctt gccggtgttg aggctctcca aggcggcgat ctcgtcggcc cgctcgcgaa
     1381 tgcggtcggc cagcttctgg acgatccggc cccgctcgta cgggctcatc cgcggccacg
     1441 gtccctcgtc gaatgccttt cgggcagcgg ccacagcccg gtcgacatcc tccttaccgg
     1501 ccttcgccac ctgggcgaca ggctggttcg tggccgggtt gtaggcggtg aacgtctcgc
     1561 cgctggccgc atccacagcc ttgccggcga tcagcagctg gaagctgcgc gtctgggtct
     1621 cggcgaccat aattccctcc ctctgtcgtc ccgcacccgg ctgactgccg aatcgggcca
     1681 ttccctggcg gcgtatggat gtattctaca gcgcccggga gaaacctcac ccccaccccc
     1741 cctctccgac gcggagaggg gggcttttgg ttcgttgagc atagaacccc tccccgcgtc
     1801 ggggagaggt tttcaacacg cgcagggcga acaaggaagc gacccgccct aatatcgccg
     1861 catcccacac gccacacggc gcatcgggcg gcgttcacag gcggcgcggc gggaccaggc
     1921 gggagtcgcg cgctggcggc ggcggggagc ggtcaacggg gcacgggttg ggcgggagat
     1981 gtgcttcggt cggccaccac gggtggtcag cagtcgagtc gtcgttgcga tccagcgtgt
     2041 ggccatcgag cgtcctccac ccaaacgaac tacgacaaac gtccgttgct tgactcggtc
     2101 agcgcccgct gctcctcgga gaggaggctg gcgcgcggtt cgttctgacc ggcgtacttg
     2161 ttgccggcct tctgccgata cggaaccagc accggcgacg agagctgctg gaaggtgaac
     2221 gagcagatcc gcatgccggg gtagagggcg accgccatcc ggcccagatt ggagagctcc
     2281 atcgtcgccg tcccgatcca gcccggctcg aagacggccg ccgtcgagtg gacggtgatg
     2341 ccgagccgcg cgatcgatga gcgaccctct aggcggccga gcaagtcgtc cggcagctcc
     2401 agcgactcga tcgtcgatgc cagcgcgaag tcacccgcct gcatgatgaa cggctcgtcg
     2461 tccttgacga cgatcgtgcg catgccctcg ccgatcgact gcggctggcg cgggtcgata
     2521 tacgagtagc gcgagtgctc gaagaccatg aaggtgttgc caagccggaa atcgaccgag
     2581 cacgagccca tgtgtttgtc gagatcgatt tccggcgtga tgcggatccg gccgttctcg
     2641 atctcgcggc gaatgtcgcg gtcggagagg atcatctgtc ggtcgtcctt cggctcccga
     2701 tgcctccggc gaggttcacc ctgcctctgc ctggacacct cgaatgatgc ggtcacctgc
     2761 gtcgtgacgg ttcggccccc agttggcttg tcagtgacgc cacgtcggac atcgcaccga
     2821 gcacctcgca ccgggaccga cgtgtacgta cagtatccct gacctgtgcc tcgtcgtcaa
     2881 gggcgacacc gggatacagg caatatgcgg ctcgtccgaa tcgcgcggtg gagcgacagt
     2941 ggcctgctat actttgctga tgcgcccgtt cacgatctca cgacaacaac agacgatcga
     3001 ccgctgtgcg tccggcgtga agcgccagta tcccgttgtg gtgctgccca acgtgtcact
     3061 cgccccacga tggagtcctg gatgtccgag caacgccggc taagcgtttc ctcgcaccac
     3121 ccgcgcaagc gaggcacgcc agcctatcaa ctacggcgaa accaacggcc gccggcgact
     3181 gaagcggacg agtcaagcca tacctcggaa ccaggcgacg aacctgtggc ggcggaacca
     3241 acggcgagtg ccgcgacgac acacgccaac agccgatcga caactggcac ggaagcgatg
     3301 ccagtcgtct caccggagcc aggcgtaccg gccacatcgg aattggtcgc ctcgcctccg
     3361 gaaaccgaat cggacgcaac agacgatccg gtgactgagg ctgaggcgac cgaggcagac
     3421 gacgctccga cgaagatcgc cgaagccgac gaaccacctg ccgaaccggt cgctgcagcc
     3481 cagcctgatg accaggccga ggccggcgaa tccgactatg ccgactacgc tcgatcggtt
     3541 gaggaagccg ctgcccgcag ccggctggag atggccccgc aggcggccgc gacggctgcc
     3601 tatcctgcca tccgaccggc tgccgcgtca ggcgctgccg tccaacgccc gaccgttcgc
     3661 cagacacgcg ggaggccggt cgtcggcagc cgtgtccgat ctggtggcgg cgggaatggc
     3721 aacggcggcc ggcgacgaac ggcgaacggc ggccacgagc gcgatccgcg cccgctgctg
     3781 aagcggccga agacctggct ggtgatcacc ggaattgtcg tcgcagtcgt cgcgattgcg
     3841 gcagtgctct gggctgccaa catcgccaga atcacctaca acgcctacaa cgaggcgcac
     3901 gtcgacaccg gcgagcggga catctacacc gtcaacgcgg aagggacgcc ggtgatcgtc
     3961 ccaaccgagc aggtccaggc ctcgctaccc aactgggaca acaaggaccc gttcaacgtc
     4021 ctgctgatgg gtatcgacga tcgcgagggc gacaccgagc cgccgcgttc cgacacgatg
     4081 atcgtcatgc gcatcaaccc ggccaacaac agcgtcgtga tgatgagcat tccgcgcgat
     4141 ctgctggtct acatcccggt cgccgaccgc tgggacaaga tcaacgccgc ctacccgatc
     4201 ggcgaggaga aggatgaaaa cggcgggcca ccggccgtca tggagacgat cgaagccaac
     4261 ttcgatgtcc ggatccacta cttcgtgacc gtcgatttca acggcttccg ctcgatcgtc
     4321 gataccgtcg gcggcgtcgt catcgacgtc cctgcgccgg tcaaggacga ccagtatccc
     4381 accgaggact tcggaatcac acgggagtac ttctcgaccg gccttcagaa gatggacggc
     4441 gagacggcac tgcgctattc acggacgcga cacaacgaca acgacatcgc ccgcggcgaa
     4501 cggcagcagc agatgctgat cgcgctccgc gagcaggcca tcgacctggg gctggtcacg
     4561 aaagccgagg agctcatcag ccagatgggc agcatggtca agaccgactt caacttcaac
     4621 cagctgctcg cactcgccaa cctcggccgc aagatcgatc cgaacgccat ctcgaaggtc
     4681 aacctctggg atgccggcgt cctcagcgag cacgccccgg aggacgagct tgatccgttc
     4741 tactttgagg ccgactggcc ggccgtctat aagctgatgg atcagtactt caacagcgac
     4801 ggcaacgcgc cgccgccccc gacgccgacc gtctctgttc cggacgaacc cctcgccagt
     4861 ccccggaccg gcgggagcaa cgtccagttc gatacgccaa tcatcgtcca gaacaacgcc
     4921 ggccagacgg cactggcacg cgccgtcggt cagatcatga ccgacaccgg cttcaccgtc
     4981 gtcgcacccg agacaggcaa cggcgagcag acgtccacca tcatctacga ctacgccgaa
     5041 cgccccgata ccgcccgcta cattgccgaa cagctcggcc tcgacgaatc gtcgatcgtc
     5101 tacggtaacg gcggcaacgg catcgtcgtt tcgctcggct cggacctcgc ctggatactg
     5161 gatcagtaat tcggtctcaa gatgacgcta gccgcacgca accggcaggg gcgagtcggt
     5221 gcctcgcccc tgcgg
//
LOCUS       DASUGJ010000047         8084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_422580, whole genome shotgun sequence.
ACCESSION   DASUGJ010000047 DASUGJ010000000
VERSION     DASUGJ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422580"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VFV93_01195"
     CDS             <1..1090
                     /locus_tag="VFV93_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5163982.1"
                     /translation="QRFARFLVRGAKGSGDRLYGQMIAMMPLMAILVPSALTRSAMLI
                     PAYEAIFESHRIERGHRLPRLIMLATATVQIQASTAVLTGGAVPVVAGALLGDLSWAR
                     WFMYMAVPNYAVLFIFSGVLFLLYRPGPMPSPVRTPDVDAAGATAAPPMTAPEWRALA
                     IISGTTLLWLTDGIHGLDPTIPALIGATALFLPLVGVLSWDDLEATSPWTIFLVTGSS
                     LSLAVALNQSGAAQWLADSIVNNLPLADLPLVPLMLSLIAMVAFINVILPNRTAVLGI
                     MIPLLMSLAGPLGLNPLTVGLIVPIIAQTTVYYPVQLATALVTFRTRHYSAGELFRAG
                     ILLTIVSILVIFVIALPWWSLLGEPIRA"
     gene            1298..3337
                     /locus_tag="VFV93_01200"
     CDS             1298..3337
                     /locus_tag="VFV93_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5163983.1"
                     /translation="MNSRQPLAAAVSLLIALALLAPLAATWLPQSAAASVMQPSLGDS
                     ASNGSLPIHPVAQSVWDAADGPVARLEVARAWLLGPAPLAMTVERYDDSPTGTRQLVY
                     FDKGRLDILDMNASPDDYWSAPGGQLVTELLSGAIQLGADRWVTRAPAEIPVVGDPDQ
                     SNGVTYATLRPLATLPRALRVDGDGSAAAESRVGADVTAVLAGDGTIDADGYNSTPVA
                     VGGYDDVTRHNIAAPFQTWGDSQVYERLYVLGHPLTEPYWVDTVVGGTLRRVLVQAFE
                     RRVLSYTPGNSDPWKTESGNVGQHYRAWRGLSDPADPALAPLVTSVSFGEELVSAAQS
                     AGVDPFLLAAVADVSSAGDPFHAIETGRRGLLGVRSELAGTSDDPASNATAGARELAR
                     LLGTTGDERAALAAYHGGASDASAAFVDQVLAARDALKARQAVPVAVDALTQPLAQVT
                     SGAAAVYANGYDSGWWTRNLTWTANWGGAIPGWFADPLGYYCTAPGYAPGDRLRLVAN
                     GLSIDCTIGGESGDPTVPAAGDGVVALNRAAFDALGLAGNNAVRVFHLGPANGQRTAR
                     ATQIDAGAGAFYASGYDVSWWETTMSRYQSWGRAVAGWSVDPNGYYCVHPGYSVGQRL
                     RLVANGVTVDCTIGDTVQQQHLAMWQSRWAIEMSWDTFNALGLPGNNSVEVYAIS"
     gene            3489..4496
                     /locus_tag="VFV93_01205"
     CDS             3489..4496
                     /locus_tag="VFV93_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163984.1"
                     /translation="MFDTPILGLIVAVAAVATLVDTWRDRAALFDARFTADERQRMLR
                     LAMFVLLPLSVLAHEGGHAIFVKLFGGEIVDFGFYMVYGYVAHVGHYTPLELAIIAFS
                     GSAINIILGVGAFAVAWFRPFRQRPAINYLLFVFAALELFNALIFYPVFDALGGVAGD
                     WSTIYTRQTPVFSTLVGLLHAALLIAAAIIWRSPRFQAGYAERTGLRRVPATPGDNEQ
                     QELTGILTVAAALATNGWRHSVQLHSDAQAGGAQVVIRWQSDGFQRALLVHATLSTMP
                     DQHVELHAAIEPDTHELPPYQRTLARIDGQPTAQELVPYIRRFLDFVDSWNGATVTTP
                     N"
     gene            complement(4520..7075)
                     /locus_tag="VFV93_01210"
     CDS             complement(4520..7075)
                     /locus_tag="VFV93_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HEX5163985.1"
                     /translation="MTEQTAITIPERSLVVLIGPSGCGKSTFARQHFLASEVVSSDAC
                     RAMLSDDENDQSATGDAFDLLHYIVGKRLARGKLTVVDATNVQPDARKPLLALAKEHD
                     VLPVAIVLDIPERVCRERNAQRPDRNFGAHVLPRQFAQFRRAVPALRREGFRYIHTLH
                     SPEEIERVVITRQPLWNDRRELTGPFDIIGDIHGCADELGELLTTLGYSVNGDSAIPP
                     EGRTAIFLGDLVDRGPATPDVLRLVMGMVAAGHALCIPGNHDSKLSRALRGRKVTVAH
                     GLQESLDQLANEPPEFRDEVATFLDGLISHYVLDGGKLVVAHAGMKERYQGRSSGRVR
                     DFALYGETTGETDEFGLPVRHNWAAEYRGKATVVYGHTPVPAAEWLNRTINIDTGCVF
                     GGALTALRYPELELLSVPALREYAAPVRPFLPDEAAPQLTAQQQHDELLDLADVSGKR
                     YIQTRSGRTVTIEAENAAAALEVMSRFAADPRWLIYLPPTMSPVETSSEPGLLEHPAE
                     AFAYYQKQGVERVVCEEKHMGSRAIVVLCRDADAARDRFGVTTGEAGIVYTRTGRRFF
                     DDVTLEATLLDRLRAAFDAAGFWDTFETNWALLDCELMPWSAKAQELLQHQYAAVGAA
                     TRHALGDTIAVLERATARGLEVDELLATQRERAELAGLYVDAYRRYCWPVASLDDLKL
                     APFHLLATEGRVQTDRDHAWQMTTLAAICAHDPGLLLATTWHEVDLADSASRDAATAW
                     WTDLTERGGEGMVVKPLQFVAQDRRGPIQPAVKVRGRDYLRIIYGPEYTRPKNLERLR
                     NRGLGAKRSLALREFTLGLEALDRFVRNEPLRRVHECVFGVLALESEPVDPRL"
     gene            complement(7418..>8084)
                     /locus_tag="VFV93_01215"
     CDS             complement(7418..>8084)
                     /locus_tag="VFV93_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5163986.1"
                     /translation="HPAQATEAAEEAAEEETIEQRISLNQQRLDAVLAALRESGAQRV
                     LDLGCGEGRLLQLLLAERQFTEIVGMDVSHRILDSAADRLRLDRLPERQRERIRLIHG
                     SLTYRDERLAGFDAAAVVEVIEHLDPPRLAAFERVIFEQARPNTVVVTTPNAEYNVRW
                     ETLPAGHYRHRDHRFEWTRADFEGWAGQIAARFSYTVRFSPIGPVDPEVGAPTQMAIF
                     ERN"
BASE COUNT         1188 a         2762 c         2667 g         1467 t
ORIGIN      
        1 ccagcgcttc gcccgcttcc tcgtgcgtgg cgcgaagggc agcggcgacc gcctctatgg
       61 gcagatgatc gcgatgatgc cgctgatggc gatcctcgtt ccgtcggcac tgacccgctc
      121 ggcgatgctg attcccgcct acgaggcgat cttcgagagc catcgaatcg agcgcggtca
      181 tcgcctgcca cggctgatca tgctggccac ggcgaccgtc cagattcagg cgtcgacggc
      241 cgtcctgacc ggcggggccg tcccggtcgt ggccggggcg ctgctgggtg atctctcatg
      301 ggcgcgctgg ttcatgtaca tggcggttcc caactacgcg gtcctgttca ttttctccgg
      361 cgttctcttc ctgctctacc ggcccggccc gatgccgtcg cctgtccgga ctcccgacgt
      421 cgatgctgcc ggtgcgactg ctgcgccgcc gatgaccgcg ccggagtggc gggcgctggc
      481 gatcatcagc gggacgacgc tgctctggct gaccgatggc atccacggtc tcgacccgac
      541 catcccggcc ctgatcggcg cgacggcgct cttcctgccg ctggtcgggg tcctcagctg
      601 ggacgatctg gaggcgacct cgccgtggac gatcttcctc gtcaccggca gctcgctgtc
      661 gctggccgtt gcgctcaacc agtccggcgc ggcccagtgg ctggccgatt cgatcgtcaa
      721 caacctgccg ctggccgatc tgccgctggt cccgctgatg ctgtcgctga tcgcaatggt
      781 ggcgttcatc aacgtcattc tcccgaaccg cacggccgtc ctcggcatca tgatcccgct
      841 cttgatgtcg ctggccgggc cgctcggctt gaacccgctg acggtcggct tgatcgttcc
      901 gatcatcgcc cagacgacgg tctactaccc ggtccagctg gccaccgccc tcgtcacctt
      961 ccggacgcgc cactactccg ccggcgagct cttccgcgcc ggcatcctgc tcacgatcgt
     1021 cagcatcctc gttattttcg tgatcgccct cccgtggtgg tcgttgctcg gggagccgat
     1081 acgggcgtga cgcgtcccgc tccccccaat tcgtcccaac aatcgaacgt gcgttcgcaa
     1141 tcatgcgcat acagtgatac aactggcggc ggtcaagttt gtgaagccct tcacgaagtt
     1201 gctgttgatg tcctgcgtgg ttctggaccg cgatccgtga agggtgagcc ggggtgtgcg
     1261 tcgtggggtc tgacgccggt ccggggaagg tttcgaagtg aatagtcgac agccgctcgc
     1321 ggcggccgtg tcgctgctga tcgccctggc gctcctcgcg ccgctggcag cgacctggtt
     1381 gccacagtct gctgccgcct cggtcatgca gccctcgctt ggtgattcag cctccaacgg
     1441 atcgcttcca attcatccag tcgctcagtc agtctgggac gctgccgacg gccccgtcgc
     1501 acggcttgag gtcgcgcgtg cctggctgct cggccctgcc ccgctggcga tgacggtcga
     1561 gcgctacgac gatagcccga ccggcacgcg acagctcgtc tacttcgaca agggccggct
     1621 ggacattctc gacatgaacg cctcgcccga cgactactgg agtgcccctg gtggtcagct
     1681 cgtgactgag ctgctctccg gcgcaatcca gcttggcgcg gaccgctggg tcacgcgcgc
     1741 accggccgag attccggtcg ttggcgatcc ggaccagtcg aacggcgtta cctatgcgac
     1801 gctccgcccg ctggcgacgc tgccacgtgc cctgcgtgtc gatggtgacg gttcagcggc
     1861 ggcagagtcc cgggttggcg ccgatgtcac cgccgttctg gcgggcgacg gcacgatcga
     1921 cgccgatggc tacaacagca cgccggtcgc ggttggtggc tacgatgatg tcacccggca
     1981 caacatcgcc gctccgtttc agacctgggg cgacagccag gtctacgagc ggctctacgt
     2041 cctcggccat ccgctgaccg agccgtactg ggtcgacacc gtcgtcggcg gaacactgcg
     2101 gcgtgtcctt gtccaggcgt tcgagcgccg cgtcctgtcg tacacgccgg gcaattcgga
     2161 cccgtggaag accgaatccg gcaacgtcgg ccagcactac cgcgcctggc gtgggctgag
     2221 cgacccggcc gacccggccc tcgcgccgct ggtcaccagc gtttcgttcg gcgaggaact
     2281 ggtgtcggcc gcgcagagcg ccggcgtcga cccgttcctg ctggcggccg tcgcggatgt
     2341 ctcgtccgct ggcgatccat tccatgcgat cgaaaccggc cggcgcgggc tgcttggtgt
     2401 ccgtagtgag cttgccggca cgagcgacga tcctgcctcg aacgcgacag ccggtgcccg
     2461 cgaactggcc cggttactgg gcacgaccgg tgatgagcgc gccgcgctgg ctgcctacca
     2521 cggtggcgcc agcgacgcat cggcggcatt cgtcgatcag gtcctggctg cccgcgatgc
     2581 gctgaaagcg cgtcaggcgg tcccggtagc ggttgatgca ctcacgcagc cgttggcgca
     2641 ggttacgtcc ggcgcggcgg ccgtctacgc gaacggctac gacagcggct ggtggacccg
     2701 taacctgacc tggactgcca attggggcgg cgctattccc ggctggttcg ccgacccgct
     2761 cggctactac tgcaccgccc ccggctacgc gccgggcgat cggctgcggc tggtcgccaa
     2821 cggcctctcg atcgactgca cgatcggcgg cgagtccggt gatccgaccg tgccggctgc
     2881 cggtgacggc gtcgtcgccc tcaaccgggc tgccttcgac gcgctcgggc tggccggcaa
     2941 caacgcggtt cgcgtcttcc acctcggacc ggccaatggt cagcggaccg cccgtgcgac
     3001 tcagatcgac gctggcgccg gtgccttcta cgcctccggc tacgacgttt cctggtggga
     3061 aacgacgatg tcccgttacc agagctgggg ccgggccgtc gccggctggt cggtcgatcc
     3121 gaacggctac tactgcgtcc acccgggcta ctcggtcggc cagcgcctgc ggctggtcgc
     3181 caacggcgtg accgtcgact gcacgatcgg cgacaccgtc cagcagcaac acctcgccat
     3241 gtggcagtcc cgctgggcca tcgagatgtc ctgggacacc ttcaacgccc tcggcctgcc
     3301 ggggaacaat agtgttgagg tgtatgcgat ctcgtagacg acgggattcg ggattcggga
     3361 ttcgggactc gggggcgttg aacggagata tctccttcga caccacccga atcccgagtc
     3421 ccgaatcccg ttcttagtaa actccacccc aagcgcccgt cgtgggtgca cggatggacc
     3481 gacctgttat gttcgacacg ccaattctgg gtctgatcgt cgccgtcgcg gccgtcgcca
     3541 cgctggttga tacctggcgc gaccgggcgg cgctgttcga cgcgcgcttc acggccgacg
     3601 agcggcaacg gatgctgcgg ctggcgatgt tcgtgctgct gccgttgagc gtgctggccc
     3661 acgagggcgg ccacgcgatc ttcgtcaagc tcttcggcgg cgagatcgtc gacttcggct
     3721 tctacatggt ctatggctac gtcgcccacg ttggccacta cacgccgttg gagctcgcga
     3781 tcatcgcgtt ctccgggtcg gccatcaaca tcattctcgg cgtcggcgcc ttcgccgtgg
     3841 cctggttccg ccccttccgg cagcggccgg ccatcaacta tctgctgttc gtcttcgcgg
     3901 cgctggagct gttcaacgcg ctcatcttct accccgtctt cgacgcgctc ggtggcgtcg
     3961 ctggcgactg gagcacgatc tacacccgcc agacgccggt cttcagcaca ttggtaggct
     4021 tgttgcacgc tgcgctgctg atcgcggcgg cgatcatctg gcgcagcccg cgcttccagg
     4081 ccggctacgc cgaacgaacg ggattgcgac gggtgcccgc aacgccaggc gacaacgagc
     4141 aacaggaact gaccggcatc ctgacggtcg ccgctgcgct ggcgaccaac ggctggcgcc
     4201 actcggtcca gctccactcg gacgcccagg ccggcggcgc gcaggttgtc atccgctggc
     4261 agtcggacgg cttccagcgc gcgctgctgg ttcacgcgac cctctcaacg atgccggacc
     4321 agcacgtcga gctgcacgcc gcgatcgagc cggacaccca cgagctaccc ccctaccagc
     4381 gcaccctcgc ccgcattgac ggccagccta ctgctcagga gctggtcccc tacatccgcc
     4441 gcttcctcga cttcgtcgac tcctggaacg gcgcaacggt cacgacaccg aattagatcc
     4501 aggagcacac ggccgggctc tacagccgcg gatcgaccgg ttcgctctcc agggcaagga
     4561 cgccgaagac gcattcgtgg acgcgacgga ggggttcgtt gcggacgaag cggtcgaggg
     4621 cttcgaggcc gagcgtgaac tcgcgcagtg ccagcgagcg tttggcgccg agaccacggt
     4681 tgcggagccg ctccaggttc tttgggcggg tgtactccgg gccgtagatg atgcgcaggt
     4741 agtcgcggcc acggaccttg acggccggct ggatcgggcc gcgtctatct tgcgcgacga
     4801 actggagcgg cttgacgacc atcccctcgc caccgcgctc ggtcaggtcc gtccaccagg
     4861 cggtcgcggc atcccggctg gcggagtcgg ccaggtccac ctcgtgccag gtggttgcca
     4921 gcagcaggcc ggggtcgtgg gcgcagatag cggccagcgt cgtcatctgc caggcgtggt
     4981 cgcggtcggt ctggacgcgt ccctcggtcg ccagcaggtg gaacggtgcc agcttcaggt
     5041 cgtccaacga ggccaccggc cagcagtagc ggcggtaggc atcgacgtag aggccggcca
     5101 gttccgcgcg ctcgcgttgg gttgccagca gctcgtccac ctccagcccg cgggccgtgg
     5161 cccgttcgag gacggcaatt gtgtcgccga gcgcgtggcg ggtggcagcg ccgacggcgg
     5221 cgtactgatg ctgcaacagc tcctgcgcct tggccgacca cggcatcagc tcgcagtcga
     5281 gcagcgccca gttggtctcg aacgtgtccc aaaagccggc cgcatcgaac gcggcgcgca
     5341 ggcggtcgag gagggttgcc tccagcgtga cgtcatcgaa gaagcgccgg ccggtgcggg
     5401 tgtagacgat gccggcctcg ccggttgtga cgccgaaccg gtcgcgggcg gcgtcggcgt
     5461 cacgacagag gacgacgatc gcccgcgaac ccatgtgctt ctcttcgcag acgacgcgct
     5521 cgacaccctg cttctggtag taggcgaacg cctcagccgg gtgctccagc agccccggct
     5581 cgctgctcgt ctcgaccggc gacatcgtcg gcggcaggta gatcagccag cgcggatcgg
     5641 cggcgaagcg gctcatcacc tccagcgcgg cagccgcgtt ctcggcctcg attgtcacgg
     5701 ttcggccgga tctggtctgg atgtagcgct tgccggagac atcggccagg tcgagcagct
     5761 cgtcatgttg ctgctgggct gtcagttgtg gtgcggcctc atccggcagg aatggcctga
     5821 ccggcgccgc gtactcgcgc agggccggga ctgagagcaa ttccagctcc gggtagcgca
     5881 gcgctgtcag cgcgccgccg aagacgcagc cggtatcgat gttgatcgtc cggttcagcc
     5941 actcggcggc cggcaccggc gtgtggccgt aaacgacggt cgctttgccg cgatactcgg
     6001 ccgcccagtt gtggcggacc ggcaagccga actcgtccgt ctcacccgtc gtctcgccgt
     6061 agagggcgaa atcccgcacc cggcccgagc tgcgcccctg gtagcgctcc ttcattccgg
     6121 catgggcgac gacgagcttg ccaccgtcga ggacgtagtg gctgatcagc ccatcgagga
     6181 aggtcgcgac ctcgtcccgg aactctggcg gctcgttcgc gagctgatcc agcgactcct
     6241 gaagaccgtg cgcgaccgtc accttgcgcc cgcgcagcgc ccgcgacagc ttgctgtcgt
     6301 ggttgcccgg tatgcagagc gcatgaccgg ccgcgaccat ccccatcacc agccgcagga
     6361 catccggcgt cgccggtccg cgatcgacga gatcgccgag gaagatggcg gttcgacctt
     6421 ccggcgggat ggcgctgtcg ccgttgacgg aatagccgag tgtcgttagc agttccccta
     6481 gctcgtcggc gcagccgtga atatcgccga tgatgtcgaa cgggccggtc agctcgcgcc
     6541 ggtcgttcca gagcggctgg cgtgtgatga cgacgcgctc tatctcctcg ggtgagtgca
     6601 gcgtgtggat atagcggaac ccctcacgcc gcagggccgg cacggcccgc cggaactgcg
     6661 cgaactggcg cggcaggacg tgcgcgccga aattgcggtc gggtcgctgg gcgttacgct
     6721 cgcggcagac ccgctcgggg atgtccagca cgatcgcgac cggcaggaca tcgtgctcct
     6781 tcgccagcgc cagcagtggc ttcctcgcgt cgggctggac attcgtcgcg tcaacgaccg
     6841 tcaacttccc gcgtgccagc cgcttcccga cgatgtagtg cagcaggtcg aaggcatcac
     6901 ccgttgccga ctggtcgttc tcgtcgtcgg agagcatcgc ccggcacgcg tccgacgaga
     6961 caacctcgct cgccaggaag tgctgcctcg cgaaggtcga cttcccgcac cccgacggcc
     7021 cgatcaggac gacgagggaa cgttctggaa tagtgatagc cgtttgctcg gtcatgtttg
     7081 tcctatgatg actgaagtgg cccgtatgtg ttcaccgcta tatgccgtca tgctccgaaa
     7141 acggaagcac cgtttcgctc tgattgagtt tccgttagct cgcgcggtgc gaggagaagc
     7201 catcctgacg ggagttgcct agcccgaccg cgtcgtctat ctcctgatct cgttctactc
     7261 gcatctggtg gatgacacct tcagcagctg gcaaggatcg atcgccgagt tcggctcgga
     7321 tcaatcgatg gatttccatc aattgctgcc acccatccgt gtcgtgtcgc tcctcgctgc
     7381 catccggaga gccatcagtg ggtgtgcttc gcatccttca gttcctctca aagatcgcca
     7441 tctgcgtcgg cgcgccaacc tctggatcga ccgggccgat cggggagaag cgaaccgtgt
     7501 agctaaagcg ggcggcaatc tggccggccc agccttcgaa gtcggcgcgg gtccactcga
     7561 agcggtggtc gcggtggcga tagtggccgg ccggcagcgt ctcccagcgg acgttgtact
     7621 cggcgttggg cgtcgtcacg acgaccgtgt tgggacgagc ctgctcgaag atgacgcgct
     7681 cgaaggcggc cagccgtggt gggtcgaggt gctcgatgac ttcgacgaca gcggcagcgt
     7741 cgaagcccgc cagccgctcg tcgcgatagg tcagcgatcc gtggatcagg cgaatccgct
     7801 cgcgttggcg ttccggcagc cggtcgagcc gcaggcggtc ggcggcactg tcgaggatgc
     7861 gatgtgagac atccatcccg acgatctctg tgaactggcg ctcggccagc agcagttgca
     7921 gcagccgtcc ctcgccgcag ccaagatcga ggacgcgctg cgcgccggac tccctgagcg
     7981 cggccaggac ggcgtcgagc cgctgctggt tgagcgagat ccgttgctcg atcgtctcct
     8041 cttcggcagc ttcttcagcg gcttcggttg cctgggcagg gtgc
//
LOCUS       DASUGJ010000048         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1812483, whole genome shotgun sequence.
ACCESSION   DASUGJ010000048 DASUGJ010000000
VERSION     DASUGJ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812483"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /gene="alr"
                     /locus_tag="VFV93_01220"
     CDS             complement(<1..1074)
                     /gene="alr"
                     /locus_tag="VFV93_01220"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5163987.1"
                     /translation="MDSDFQGAIDRFQSGTHALIDLDAYERNIGVLLSIAGERVDLMA
                     VVKADAYGHGAVECGRAAVAAGARMLGVARIDEALYLRQSGVTAPIVLIGPPAIAEIP
                     ATLQHDITLAIGSRQSVDGLLAGMGQGLRARVHVKVDTGMNRYGFRPEDVTAVVEELA
                     SNDRITVEGVFTHFSSADERDPAPTERQIERFWASVAGLEARGLRPEYVHMANSAAIL
                     TGHFEGTNLVRSGIATYGLSPSDEVIVDERFQPVLTLRSIVVRRGTLPVGEGVSYGRT
                     YRAAATEQIAAVPVGYADGLPRRLRNQGWFVVRGERTPIRGSVCMDQTVVSVPEDTVE
                     GDIVRVIGDGSLGEMTFDDIASMI"
     gene            complement(1075..1533)
                     /locus_tag="VFV93_01225"
     CDS             complement(1075..1533)
                     /locus_tag="VFV93_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5163988.1"
                     /translation="MAAEVPVANIMTTSVVSVSRTTEVTEVARLMVEHAVSGLPVVEG
                     DRVVGIVTERDLIAQEIEFDPPAYGTFLDAIFRLPWDKSDEELRRILATRAEQLMSEE
                     VITVQPTATVRDAASLMYKRRVNPLPVVDEQGRMVGIISRSDIVQLLTEM"
     gene            complement(1557..>2622)
                     /locus_tag="VFV93_01230"
     CDS             complement(1557..>2622)
                     /locus_tag="VFV93_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX5163989.1"
                     /translation="AFRADVTLCLGLPKIGLIHEPAATHTGELELLDIGIAPPPGGDR
                     PELIDERTVRSWLPRRNASAHKHQTGTVLVIGGAPTYYGAPRLTAEAAARAGAGLVCV
                     AAPASIIPVIAGQVPELVMLPLDESAAEAARQIMDWIDGRGGHMDACIVGPGLGQTPH
                     ATNLLERLLGDPNSGIVSSRALRGKQPAFLLDADALNWIAKRGSFPAGVPSKTAVMTP
                     HAGELGRLLAEPTDDLLADTIGSARRAARVFDQTLVFKSGYSVVATPEGAAWVSPRSA
                     PELATAGTGDVLAGLIGGILAQGMSPVDAAATALFAGAHAGRMARADLGTQAVMATDV
                     IDRIAPALRRLSEPNWRGDE"
BASE COUNT          466 a          909 c          797 g          450 t
ORIGIN      
        1 gatcatcgac gcgatatcgt cgaaggtcat ctctcccagc gatccgtcgc cgatgacgcg
       61 tacgatgtcg ccttcaaccg tgtcctcggg gaccgagacg accgtctgat ccatacagac
      121 gctgccccgg attggtgtac gttcgccccg tacgacaaac cagccctgat tgcggagccg
      181 tcgtggcagg ccgtccgcgt agccgacggg cacggccgcg atctgctcgg tcgccgccgc
      241 tcgatacgtc cggccgtatg aaacgccctc ccccaccggc agcgtcccac gccgcacgac
      301 gatcgagcgt agcgtcagga cgggctgaaa gcgctcatcg acaattacct cgtccgacgg
      361 cgataggccg taggtcgcga tgccactgcg gacgagattc gtcccctcga agtggccggt
      421 aaggatcgcg gcgctgttcg ccatgtggac atactccggt cgcagtccac gtgcttcgag
      481 cccggcgacg gatgcccaga accgttcgat ttgccgctcg gtcggtgccg ggtcacgctc
      541 gtctgcagac gagaaatggg taaagacgcc ctcgaccgtg atccggtcgt tcgacgccag
      601 ctcctctacg acggccgtga cgtcctcggg acgaaacccg tagcggttca tgccagtgtc
      661 aactttcacg tggacacgtg cccgcagccc ctgccccatt ccggccagga gtccgtcgac
      721 cgactggcgg gaaccgatcg cgagtgttat gtcgtgttgc agcgttgccg gtatctcggc
      781 gatcgcgggc gggccgatca ggacgatcgg agccgtcact cctgactggc gcagatagag
      841 cgcctcatcg atccgggcca cgcccagcat ccgcgcaccg gcagcaacgg ccgcccgccc
      901 acattcgact gcgccgtggc cgtacgcgtc ggctttgacg accgccatca ggtcgacacg
      961 ctcaccggcg atggacagca ggacaccgat gttgcgctca tacgcgtcaa gatcgatcag
     1021 cgcgtgcgtc cccgattgaa agcgatcgat cgcaccctga aagtcgctat ccacctacat
     1081 ctccgtcaac agctgcacga tgtccgagcg cgaaataatg ccgaccatgc gtccctgctc
     1141 atcaacaact ggcagcggat tcactcgccg cttgtacatc aggctagcgg cgtcgcgaac
     1201 cgttgccgtt ggctggacgg tgatgacctc ttccgacatg agctgttcgg cgcgtgtcgc
     1261 aaggatgcgc cgcaactcct cgtcggactt gtcccacgga agccggaaga tcgcatccag
     1321 gaacgtccca tacgccggag gatcgaattc gatctcctgg gcgatcagat ctcgctcggt
     1381 gacgatcccg acaacccggt caccttccac gaccggaagc cccgaaaccg catgctcgac
     1441 catcagtcgg gcaacttcag tgacctcggt cgtccgggag acgctgacga cactcgtcgt
     1501 catgatattc gccactggca cctcagctgc catcacaact cctcacgcgg actgctctat
     1561 tcatcaccac gccagttcgg ctcactgaga cgcctcagcg ctggcgcgat ccggtcgatc
     1621 acatccgtcg ccatcaccgc ctgagttccc agatcggcac gggccattct accggcatgc
     1681 gcgccggcga acagcgccgt tgcggcggca tcgaccggcg acatgccctg cgcgagaatc
     1741 ccgccgatca accctgccag cacgtcgcca gttccggccg tcgcgagctc cggagcggac
     1801 cgcggcgaaa cccaggctgc gccctccggt gtagcgacaa cagagtaacc actcttgaag
     1861 acgagcgtct ggtcgaagac tcgggccgcc cggcgcgcac tcccgatcgt atcggcaagc
     1921 aggtcgtccg tcggttcggc aagcagccgc ccaagctcgc ctgcgtgcgg agtcatcacg
     1981 gcagttttcg atggcactcc ggccgggaac gagccgcgct tggcgatcca gttgagcgcg
     2041 tctgcgtcaa gcaggaatgc tggctgcttg ccacgcagcg cgcgtgatga aacgatcccg
     2101 ctgtttggat cgcccagcag ccgttccagc aggttggtag cgtgcggagt ctggccgaga
     2161 cccgggccga cgatacatgc gtccatgtgc ccgccgcgac catcgatcca gtccatgatc
     2221 tgccgggccg cctcggcagc cgattcgtca agcggcagca ttaccagttc gggaacctgt
     2281 ccggcaataa cggggatgat actggccgga gccgccacgc agacaagccc cgccccggct
     2341 ctggcggctg cttcggcggt caacctcggg gcgccgtaat acgtcggcgc cccgccgatc
     2401 acgaggacgg tgccggtctg atgcttgtgg gcagaggcgt ttcgccgcgg cagccagctc
     2461 cgcaccgtgc gctcgtcgat cagctccggt cgatccccgc ccggcggtgg cgcgatgccg
     2521 atgtcgagca gttccagttc gcccgtatgg gtcgctgccg gttcgtggat cagcccgatc
     2581 ttcggcaagc cgaggcagag ggtcacatcg gcgcggaagg cg
//
LOCUS       DASUGJ010000049         3208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_145395, whole genome shotgun sequence.
ACCESSION   DASUGJ010000049 DASUGJ010000000
VERSION     DASUGJ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145395"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..845
                     /locus_tag="VFV93_01235"
     CDS             321..845
                     /locus_tag="VFV93_01235"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5163990.1"
                     /translation="MTGIEQTGDERVVLPVARPAPSERLDPRALTAWRISGLLSSLLL
                     LALAAAVSWLLWYLEQRLWLVALPLLVALVAGVIATWVAPAVQWRRWRYAVTERDIEL
                     QRGLIVVTRTLIPIVRVQHVDTRQGPILRRFDLAGIAIATAAGTEEIPALAVEVADDL
                     RHRISDLAGLADDV"
     gene            838..2289
                     /locus_tag="VFV93_01240"
     CDS             838..2289
                     /locus_tag="VFV93_01240"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5163991.1"
                     /translation="MSEPHRLHPAAIALMAFQIVRQFAIPALIPIAIRLFNEGLGAVS
                     LETAVVTIVILVALLGLSAAYGVLAWHRFTYRVEAGELRIEHGIVNRQRRFIPLERIQ
                     TFDSSQGLLQRLLGVVSVRVETAGGGGGSPEVSLPGVSQGDSERLRQALAAGRAGAAQ
                     AAPELETARAPAANVWKLSATDLLLAGSTGGRAGVALSIILAAVSLADNLIPYEQIGG
                     LAERVTGAVVVLGLALALAAIVWLLGVAGTILAHAGFTITQQGDHLLIERGLLERRRA
                     TIPIERIQAIRIIEGVLRQPFGLVELRVESAGYGPKAGESTVLCPLLRRRDVQQFLTR
                     MAPSLAVAGTFQPLPRRARRRYALQLPVLIQPLVLIVPVIYFFYPLGLLGLLLLPAVV
                     ALGLWQYHDAGWAVEGDTLLLRWRAIARTTAIVPRRRIQLGSTHATPFQRRVQLSTLT
                     VRVPSGPGGATFGLLHMDHETALRLLAWLGPRR"
     gene            2357..>3208
                     /locus_tag="VFV93_01245"
     CDS             2357..>3208
                     /locus_tag="VFV93_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEX5163992.1"
                     /translation="MAAPDRVYQLVERFDANREQYRRPDYNETQVRVNFINPLFEALG
                     WDVRNRRGEAPSAMEVIHEDDVRVGKAHKAPDYGFYANQERKFFVEAKKPAVNLREDA
                     SPAFQLRRYAWSAKLAVSVLSDFEEFAIYDARYQPTLHDSAAVARTLHLRYDQYVDRW
                     DDIAGLFSKEAVLDGAIEQRFPKDKSIRGTATVDRAFLTEIEHWRELLARNIALRNPD
                     LSGRELNFAVQMTVDRIIFLRMAEDRGIEEYERLLGIAKGEGVYARLRDLFRQADERY
                     NSGLFHFT"
BASE COUNT          439 a         1024 c         1202 g          543 t
ORIGIN      
        1 cgaacatgcc aagctgatag cgggcggtga cggtcgccgg gagtcgggcc agcagccagt
       61 cataggcgat acgcatcgaa tcgatccttc gtcaatgctg aactgctcca gtgtcggagc
      121 atgtattgcc attaaagagg aatgcatgcc acgaaggaac ccgtaagtgt gcgatctggc
      181 gcggttgaga cggttttgtg agagaactgg cgacgatgcg aggctccgtg gcgcgcgaca
      241 ggcggtgatg gcgtaggctt gcgggatggc gccggtccag cgggcattcc ggcgccggcg
      301 acgacgattg agacggtggt gtgacaggga tcgaacagac gggcgatgag cgcgttgtgt
      361 tgccggttgc gcggccggcg ccgtccgagc ggctggaccc acgggcgctg acggcctggc
      421 gcatctccgg gctgctctcg tcgctgctgc tgctggcgct ggcggcggcg gtcagctggc
      481 tgctctggta tctggagcag cgcctgtggc tggtggcgct gccgctgctg gtggcgctgg
      541 tggccggggt gatcgcgacc tgggttgcgc cggcggtcca gtggcggcgc tggcgctacg
      601 ccgtcaccga gcgggacatc gagctgcagc gcgggctgat cgtcgtgacg cggacgctga
      661 tcccgatcgt gcgggtccag catgttgaca cgcggcaggg gccgatcctg cggcgcttcg
      721 acctggccgg gatcgcgatc gcgacggcgg ccgggacgga ggagatcccg gcgctggcgg
      781 tggaagttgc cgacgatctg cgccaccgca tctctgacct ggcgggcctt gccgacgatg
      841 tctgagccgc accgcctgca cccggccgcg atcgcgctga tggcgttcca gatcgtgcgc
      901 cagttcgcga ttccagcgct cattccgatc gcgatccggc tcttcaacga ggggctgggc
      961 gcggtcagcc ttgagacggc tgtcgtcacg atcgtgatcc tcgtggcatt gctgggcctc
     1021 tcggccgcct atggcgtgct ggcctggcac cgcttcacct accgggtcga ggcgggcgag
     1081 ctgcggatcg agcacggcat cgtcaaccgc cagcggcgct tcatcccgct cgaacggatc
     1141 cagacgttcg actccagcca ggggttgctc cagcggctgc tgggcgtcgt cagcgtccgg
     1201 gtcgagacgg ctggtggtgg gggcgggtcg ccggaggtgt cgctgccggg tgtctcgcag
     1261 ggggatagcg agcggctgcg ccaggcgctg gcggccggcc gggctggggc agcccaggcc
     1321 gcgccggagt tggagacggc ccgcgcgccg gcggccaacg tctggaagct gagcgcgacc
     1381 gacctgctgc tggccggcag caccggtggg cgggccgggg tcgcgctctc gatcatcctg
     1441 gcggccgtct cgctggcgga caacctgatc ccgtacgagc agatcggcgg gctggccgag
     1501 cgggtgacgg gcgcggtcgt cgtgctgggg ctggcgctgg cgctggcggc catcgtctgg
     1561 ctgctgggtg tggccgggac gatcctggcg cacgcgggct tcacgatcac ccagcagggc
     1621 gaccacctgc tgatcgagcg cggcttgctg gagcggcggc gggcgacgat cccgatcgag
     1681 cgcattcagg cgatccgcat catcgagggc gtgctgcggc agccgttcgg gctggtcgag
     1741 ctgcgggtcg agagcgccgg ctacggccca aaggcgggcg aatcgacggt gctgtgcccg
     1801 ctgctgcgcc gtcgcgacgt gcagcagttc ctgacgcgga tggcgccgtc gctggcggtt
     1861 gccggcacgt tccagccgtt gccgcgccgg gcccgtcgcc gctatgcgtt gcagctgccg
     1921 gtcctgatcc agccgctggt gctgatcgtg ccggtgatct acttcttcta tccgctgggg
     1981 ctgctgggcc tgctgttgct gccggccgtc gtggcgctgg ggttgtggca gtaccacgac
     2041 gccggctggg cggtggaggg tgacacgctg ctgctgcgct ggcgggcgat cgcgaggacg
     2101 acggcgattg tcccccgccg ccggatccag ctcggctcga cccacgccac cccgttccaa
     2161 cgccgggtgc agctctcgac tctcaccgtc cgggtcccct ccggccccgg cggcgcgacg
     2221 ttcggcctgc tgcacatgga ccacgagacg gccctgcgcc tgctggcctg gcttgggccg
     2281 cggcgctgat cgttgcccgc cgcgccattc gcgttatgat gcgacgcacg aattgtcgcc
     2341 gcggacgggg gtggggatgg cagcgccgga tcgggtctat cagctggtcg agcggtttga
     2401 cgcgaaccgg gagcagtacc ggcggccgga ctataacgag acgcaggtgc gggtcaactt
     2461 catcaacccg ctcttcgagg cgctgggctg ggacgtccgc aaccggcgtg gtgaggcgcc
     2521 gagcgcgatg gaggtcatcc acgaggacga tgtgcgcgtc ggcaaggccc acaaggcgcc
     2581 cgactacggc ttctacgcca atcaggagcg caagttcttt gtcgaggcca agaagccggc
     2641 cgtcaacctg cgcgaggacg cctcgcccgc tttccagttg cgccgctatg cctggtcggc
     2701 caagctggcg gtcagtgtcc tgtcggactt cgaggagttt gcgatctacg acgcgcgcta
     2761 ccagccgacg ctgcacgact cggccgctgt cgcgcggacg ctgcacctgc gctacgacca
     2821 gtacgtcgac aggtgggacg atatcgccgg tctcttctcg aaggaggccg tcctggatgg
     2881 cgcgatcgag cagcgcttcc cgaaggacaa gagcatccgt gggacggcca cggttgaccg
     2941 cgccttcctg accgagatcg agcactggcg cgagctgctg gcgcgcaaca tcgcgctgcg
     3001 taatcccgac ctctccgggc gggagctgaa cttcgccgtc cagatgacgg ttgaccggat
     3061 catcttcctg cggatggccg aggaccgcgg gatcgaggag tacgagcggc tactggggat
     3121 cgcgaagggc gagggagtct acgcccggtt gcgcgacctg ttccggcagg cggacgagcg
     3181 ttacaactcg gggctgttcc acttcacc
//
LOCUS       DASUGJ010000050        10127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1256566, whole genome shotgun sequence.
ACCESSION   DASUGJ010000050 DASUGJ010000000
VERSION     DASUGJ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10127
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256566"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1106
                     /locus_tag="VFV93_01250"
     CDS             216..1106
                     /locus_tag="VFV93_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5163993.1"
                     /translation="MAAVRVENLHFRYPVFDPDATGVGGEEPGYALAGIELEIAEGEL
                     LGISGTTGSGKTTLCLALNGLVPQQTSGTIRGDVWIGDWNTKRVPVPQLATRVGLVFQ
                     DPEANLLGLTVEDEVAFGPENLAVEPREIEERVGWALDVVGMSAFRRRPAALLSGGQK
                     QRVGIAAVLAMLPQVLVLDEPTAELDPIGKREITDVISGLRERERGMTVVMVESDAAT
                     LARLADRLAIMDAGAIVALDEPRALYSHPAPLIERGVPVPDVSEVALLLNRRLGTDWS
                     FVTHDEAMAALANDLRATSR"
     gene            1103..1951
                     /locus_tag="VFV93_01255"
     CDS             1103..1951
                     /locus_tag="VFV93_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5163994.1"
                     /translation="MSDPLVRVESVSFAYPGDAGEPPRIVLHDVSLAIEPGEFIALIG
                     QNGSGKTTLARHLNGLLKPSAGRVLVGGVDSRTVPVGELARQVGYVFQNPDHQLFLPS
                     VEGEIGWGPRQLGLSGAELDERVRSTLERFGLTVWAEHHPAMLGRGLRRLTALAAVSA
                     MGPHVLVLDEPTGGLDRRLKGALMVILRGLTAEGYAVVLITHEMALVAEHAARVVVLH
                     AGRIVADEAPTTLFHRLDLLADAGIEPPPVARLAHELRCAGMPAEVATAASFVDAFVE
                     LREAGR"
     gene            1948..2751
                     /locus_tag="VFV93_01260"
     CDS             1948..2751
                     /locus_tag="VFV93_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX5163995.1"
                     /translation="MSAAAFDFYVAGRSRLHRLDPRVKLALVAAGSVVLFLWLSLPLL
                     AGVIIGTHLWLMASGYPWSKLLAIWRAIAYLLLMIVVIWPVFDKSGDLLFSIGPLDIH
                     DDALLRGVANAARIAAINFIFVLWIGTTDARELVRGFVRLGLPFRWGMALTIGLRFIP
                     TFAATFATVSDAQQARGLVLRGWWGRRVRTMLPIFIAALVSALRASEQLAMTLEARGF
                     GAGRRRTTLRDIRMAPFDWLVLALTLLVSLTLLWLTLTAGFGRELLAVT"
     gene            2807..3502
                     /locus_tag="VFV93_01265"
     CDS             2807..3502
                     /locus_tag="VFV93_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5163996.1"
                     /translation="MTAPHLDLLGPLETVEVRLPESGVCYQITRPTDIDRLIDAMADD
                     PEEVLPHWAEIWPSGIALADAMLAAPEVVRGRRALELGCGLGTTATAALQAGAELTVT
                     DYAEGALALCRVNTRANAGRAPAAMQLNWRRPSDALLELAGVGFPVVLAADVLYEARD
                     IEPLLALTARIVAPGGTLWLAEPGRPIAREFVARAEAGGWRSSQRRHGGPWPDERDGS
                     VVVTVYRLERDTC"
     gene            complement(3558..3833)
                     /locus_tag="VFV93_01270"
     CDS             complement(3558..3833)
                     /locus_tag="VFV93_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX5163997.1"
                     /translation="MSTRVSIVLPDEVLAELDQIAGKRHRSEFIAEAIREKLNLARRT
                     RAVRETVAILPGDEIPVWHSAEEVSAWLRASRQRDVERLTPQEHTSA"
     gene            complement(4019..5416)
                     /locus_tag="VFV93_01275"
     CDS             complement(4019..5416)
                     /locus_tag="VFV93_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163998.1"
                     /translation="MKAAFTTFGLVCLMLLSACSIGGSDSDDPTATVTAAATESASPT
                     ATAEATETPAASPTTTSTRAAASPTETATGESASPEATQAGLDPKLAEEIDEIEQRIE
                     KIRKLDLLKTVPISVISREQLRQNLIDDVNADYSQEEADEDTQELWLLRLIDDPTLDL
                     YQFQIDLLSEQVLGYYDPERDEMFIVSDEDGLSGLAKFTLSHELDHALQDQYYDLQKV
                     RFYDQGDNDRDTAAVALIEGDAELAQTAYAQTMDPGELVDMLSEASSLSSEVVDNAPP
                     YLRESLYFPYDQGLKFVQTLYAVDGDDTLNQAFTDPPRSTEQILHPEKYLDQRDDPQA
                     VELPDLSAALGAGWEQRDTDSLGEFDLSVLLRENGAGDYAEAAAGWDGGRYAYYAGDS
                     GSLVAVKTVWDSPDEATEFYDAMLETLAGPLDGEIGDAGQGRYLGLREVDGAVWFISS
                     TDRATVESALTAIGE"
     gene            complement(5668..5991)
                     /locus_tag="VFV93_01280"
     CDS             complement(5668..5991)
                     /locus_tag="VFV93_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5163999.1"
                     /translation="MREHARNYVIYLRRRVEDFADADRPVPKWSDLDHEAVKVVLDEF
                     ERLQHANQSAGDTLRKHGLAPSRPKISTGDRDLPFDSDTGSRLLLLACGVLLGAFLRE
                     IIAVF"
     gene            complement(6142..7311)
                     /locus_tag="VFV93_01285"
     CDS             complement(6142..7311)
                     /locus_tag="VFV93_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164000.1"
                     /translation="MPGERDQERLGNVLRDAYRDVVPTQDERQRHLTSIEQTLPGMKD
                     IPWRRQRGLHEAIVVITVLALVVGGFAFWESRDTSSGTPEPVVGAPVSVTVTPTAATP
                     VASGCAVTPTNPSPPSRFLSDPYHKEWYGENEFWISPISIDGINPSLPESETRWFTGG
                     TPVLGDWPFQAGDVFSITAKRLDGPADPVDVQQTSDSEINNIVVPFPEAGCWELTAGV
                     RDLSMTIVVKVLPLTDRPDMRQAIERRDAVLPYHAPDSCGPGWGDPVDRLGNYGPAYW
                     MDGTGISIRSTDGLLWANQPATLDWFPDEWGDITIEGHALDSGMKLGYAADNIQQIGV
                     QGNLWRSSLIFGESGCWELTATVGDATATFTVWVYPAECRREPGEPLPPACRPPV"
     gene            complement(7301..7876)
                     /locus_tag="VFV93_01290"
     CDS             complement(7301..7876)
                     /locus_tag="VFV93_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5164001.1"
                     /translation="MKEAVSAEPELSTMSDEQIARLALRQRHAFGELYVRYADRIFRY
                     AAGRTGSRTIADDVLNDTMVGALEGLHRYDPDKGSFASWLFTIASRRIADDARGRVQQ
                     RRFLSRRTLSALNDDLLDALIRSEDHERVRSAVQRLPSRQREIVLLRYVAELSFRDVA
                     RVLGISEGAARMRVNRALHRLAEEMGVIDAG"
     gene            7958..9076
                     /locus_tag="VFV93_01295"
     CDS             7958..9076
                     /locus_tag="VFV93_01295"
                     /inference="COORDINATES: protein motif:HMM:NF040505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsO family NAD(P)H-dependent flavin-containing
                     monooxygenase"
                     /protein_id="HEX5164002.1"
                     /translation="MARSAQQIDLHEASGTMDEKSRDVVVIGGGQTGLAAGYYLRRTG
                     LSFVILDAEVGPGGAWRHCWESLRLFSPARWSSLPGYVMPGGTEYYPDRSEVLAYMAE
                     YERRYRLPIERPVLVGAVRDGGEQVIVETDRGVWQARAVVSATGSWRAPYIPEIEGRD
                     QFRGEQIHSAEYESPMAFVGRRVLVVGGANSGAQIMAELSLVADATWVTRREPVFLPD
                     NVDGRVLFERATERYLAAKEGRQLPPESGSGKFLGDVVMLPPVREARDRGDLSSVRMF
                     SRFTRDGVVWQDGHGERIDAVIWCTGFRTALDHLAPLGVVDASGQVETDGTRSVRDPR
                     LWLLGYGEWTGYASATLIGIGRSARATAEEILGVLSAA"
     gene            9087..>10127
                     /gene="argS"
                     /locus_tag="VFV93_01300"
     CDS             9087..>10127
                     /gene="argS"
                     /locus_tag="VFV93_01300"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5164003.1"
                     /translation="MEYAIDRCERDILAGLTATGLVPEEALTVEEPKAGIAADLTFAA
                     FGVARELGVPPPQLATEIAERLRFEPGSLAGTATAAGPYVNVSVDPERFTGAVLEDIA
                     RGGDRYGHDDVGAGQSIVIDYSSPNIAKRMHAGHIRSTIIGQALVNMLSALGYRVIRD
                     NHIGDWGKSFGVLLAAIEREGYPAGDGELLLAALEDLYALQSALIAQDPEQDARAREW
                     SLRLERGDPEARRIWQEIVDLTLAINQTSYDRLGVSFDHVHGESFYEPMLAEVIDDAL
                     ASGAATRDPSGAVVVDAGEGLPTFLLQRSDGGTLYHTRDVATARYRVHEFQPAKVLYV
                     IGAPQELYLRQLF"
BASE COUNT         1502 a         3181 c         3642 g         1802 t
ORIGIN      
        1 cgctcaccgc ccccgatcca ccgctggcgt atgccgtcag catcgccgct cgacccgtcc
       61 tcaggacccc acgctatgcc aaacgcaagt gccaacgccg ttcatccgat taagttggaa
      121 caggtggtca ccgacattgc ccctacccat tggcggattg gcgggtgcgt taccattccc
      181 ggttggggaa tgggtcgctg gtggtcggag cattgatggc cgctgttcgg gttgagaatc
      241 tgcattttcg gtatcccgtc ttcgatccgg atgcgaccgg ggttgggggc gaggagccgg
      301 ggtatgcgct ggcggggatc gagttggaga ttgccgaggg cgagctgctg gggatcagcg
      361 ggacgaccgg gagcgggaag acgaccctct gcctggcgct gaatgggctg gtgccgcagc
      421 agacgagcgg gacgattcgt ggggatgtct ggatcggtga ctggaacacg aagcgggtac
      481 ctgtgccgca gttggcgacg cgggtggggc tggtctttca ggatcctgag gcgaacctgc
      541 tggggctgac agtcgaagat gaggtcgcct tcgggccgga gaatctggcg gttgagccgc
      601 gtgagatcga ggagcgggtc ggctgggcgc tggacgtcgt cgggatgagc gcgttccggc
      661 ggcggccggc tgcgctgctc tccggcggac agaagcagcg ggttgggatt gcggccgtcc
      721 tggcgatgct gccgcaggtg ctggtgctgg atgagccgac ggccgagctg gacccgatcg
      781 gcaagcgcga gatcaccgat gtcatcagcg gcctgcgcga acgggagcgg gggatgacgg
      841 tcgtgatggt cgagagtgac gcggcgacgc tggcgcggct ggccgatcgg ctggcgatca
      901 tggacgccgg cgcgatcgtc gccctcgacg agccacgggc gctgtactcc caccccgcgc
      961 cgctgatcga gcggggcgtg ccggtgccgg atgtcagcga ggtggcgctg ctgctgaacc
     1021 ggcggctcgg gacggactgg tcgttcgtga cgcacgacga ggcgatggcg gcgctggcga
     1081 acgacctgcg ggcaaccagc cggtgagtga tcctctcgtt cgggtagagt ccgtctcgtt
     1141 cgcctatccg ggcgatgccg gcgagccgcc gaggatcgtc cttcacgatg tgtcgctggc
     1201 gatcgagccg ggagagttca tcgcgctgat cggccagaac ggctccggca agacgacgct
     1261 ggcgcggcat ctgaacgggc tgctgaagcc gtcagccggg cgggtgctgg ttggcggggt
     1321 cgattcacgg actgtgccgg ttggcgagct ggcgcggcag gtcggctacg tcttccagaa
     1381 tcccgatcac cagctcttcc tgccctcggt cgagggggag attggttggg gaccgcgcca
     1441 gctcgggctg tctggcgccg agctggacga gcgggtgcga tcgacgctgg agcggttcgg
     1501 gctgaccgtc tgggcagagc atcacccggc gatgctgggg cgcggcttgc ggcggctgac
     1561 ggcgctggca gccgtctccg cgatggggcc gcatgtgctg gtcctcgacg agccgaccgg
     1621 cggcctcgac cggcggctga agggcgcgct gatggtcatc ctgcgtgggc tgaccgccga
     1681 gggctacgcc gtcgtcctga tcacgcacga gatggcgctg gtcgccgagc atgcagcgcg
     1741 ggtcgttgtc ctgcacgccg gaaggatcgt cgcggacgaa gcaccgacga cgctgttcca
     1801 ccggttggac ctgctggccg acgcgggaat cgagccgccg ccggtcgcgc ggctggcgca
     1861 cgagcttcgt tgcgcgggga tgccggcgga ggtggcgaca gcggcgagct tcgttgacgc
     1921 ctttgtcgaa ctgcgtgagg ccggccggtg agcgcagctg cgttcgactt ctacgtggca
     1981 ggccgctcgc ggctccaccg gctggacccg cgcgtcaaac tggcgctggt ggcggccggc
     2041 agcgtcgtcc tcttcctctg gttgagcctc ccgctgctgg ccggcgtcat catcggaacc
     2101 cacctctggc tgatggcgtc aggctacccg tggagcaagc tgctcgcgat ctggcgtgcg
     2161 atcgcgtacc tgttactcat gatcgtcgtg atctggccgg tcttcgacaa atccggcgac
     2221 ctgctcttct cgatcggccc gctcgacatt cacgacgacg cgctgctgcg gggtgtcgcc
     2281 aacgcggccc ggatcgcggc catcaacttc atcttcgtcc tctggatcgg gacgaccgac
     2341 gcccgcgaac tggtgcgcgg cttcgtccgg ctcgggctgc cgttccgctg ggggatggcg
     2401 ctcacgatcg gcctgcgctt catccccacc ttcgcggcga ccttcgcgac cgtctccgac
     2461 gcccagcagg cgcgtgggct ggtcctgcgc ggctggtggg gccggcgggt acggacgatg
     2521 ctgccgatct tcatcgccgc gctcgtctcc gccctgcgcg ccagcgagca actggcgatg
     2581 acgctcgaag ctcgcggctt cggcgctggc cgccggcgga cgacgttgcg tgacatccgg
     2641 atggcgccgt tcgactggct cgtgctggcg ctgacgctgc tggtctcgct cacactgctc
     2701 tggctgacgc tgacggccgg cttcggccgc gaactgctgg cggtaacgtg agcttcaacg
     2761 gttgaattgt tcttctgtca tccggcatgg atcgcgagga gtgtcgatga ccgcgcctca
     2821 tctggatctg ctgggaccac tggagaccgt cgaggtccgt ctgccggaga gcggcgtctg
     2881 ctaccagatc acgcggccaa ccgacatcga ccggctgatc gacgcgatgg cggacgatcc
     2941 tgaggaggtc ctgccgcact gggccgagat ctggccgagt ggcattgcgc tggcggacgc
     3001 gatgctggct gcgccggagg tcgttcgcgg gcggcgggcg ctggagctcg gttgcgggct
     3061 cgggacgacg gcgacggccg cgctgcaggc cggcgcggag ctgacggtta ccgactatgc
     3121 cgagggtgcg ctggcgctgt gtcgcgtgaa cacccgcgcc aacgccggcc gcgcgccagc
     3181 ggcgatgcaa ctcaactggc gtcgacccag cgatgcattg ctggagctgg ccggagtcgg
     3241 ctttccggtc gttctggcgg ccgatgtgct ctacgaggcg cgcgatatcg agccgctgct
     3301 ggcgttgaca gcgcggatcg tcgcgcccgg cggcacgctc tggctggccg agcccggccg
     3361 gccgatcgcg cgtgagttcg tcgcccgcgc ggaggcgggt ggctggcgca gcagtcaacg
     3421 gcgccacggc ggcccatggc ccgatgagcg ggatgggtcg gtcgtcgtga cggtctatcg
     3481 gcttgagcgt gatacgtgct gagtagcgag catccggcgg gtatgtgcag cgtcgtgcgc
     3541 tcgacactca gcacgtatca ggcgctggtg tgttcctgcg gtgtgaggcg ttcgacatcc
     3601 cgctggcggc tggcgcgcag ccaggccgag acttcttcgg cactgtgcca gacggggatc
     3661 tcgtcgccag gcaggatggc gacggtttcg cggacagctc tggtccgccg tgcgaggttg
     3721 agcttctcgc ggatggcctc cgcgatgaac tcgcttcggt gacgtttccc ggcgatctgg
     3781 tcgagctcgg ccagcacttc gtcgggtagg acaatactca cacgtgtaga catcgtgtcc
     3841 tcccattcgg tcacttacac gatagcacat gcggataacg tcaggtttca cgagatcatt
     3901 ctcgtgggtc tgcggaaccc gctcaaacgg cgtcggtgga gagcagggcg agacagtgcc
     3961 tcacccctgc cggaggggtg gcctccatag atcaagaatg caacgtacgg ttcggtcgtt
     4021 actcgccgat cgcggtcagg gctgattcaa ccgtcgcgcg gtcggtgctg ctgatgaacc
     4081 agacggcgcc gtcgacctcg cgcaggccga ggtagcggcc ctggccggca tcgccgatct
     4141 cgccgtcgag tgggccggcg agcgtctcca gcatggcgtc gtagaactcg gtcgcctcgt
     4201 cggggctgtc ccagacggtc ttgacggcca ccagcgagcc gctgtcaccg gcgtagtagg
     4261 cgtagcgtcc tccatcccag ccggcggcgg cttcggcgta gtcgccggcg ccgttctcgc
     4321 gcagcaggac cgagaggtcg aactcgccga gactgtcagt gtcgcgttgc tcccaaccgg
     4381 cgccaagcgc ggcgctcagg tcgggcagtt cgacggcctg cggatcgtcg cgctggtcga
     4441 ggtacttctc cggatgcagg atctgctcgg tcgagcgcgg cggatcggta aatgcctgat
     4501 tgagcgtgtc atcgccgtcc accgcgtaga gcgtctggac gaacttcaac ccctgatcat
     4561 aggggaaata gaggctctcg cgcaggtagg gtggtgcgtt gtcaacgacc tcggacgaga
     4621 gcgaactggc ttcgctcaac atgtcgacca gctcgccggg gtccatcgtc tgggcgtatg
     4681 ctgtctgggc cagttcggcg tcgccctcga tcaacgcgac ggcggccgtg tcacggtcgt
     4741 tgtcgccctg atcgtagaag cgcaccttct ggagatcgta gtactggtcc tggagggcgt
     4801 ggtccaactc gtggctgagc gtgaacttgg ccagcccgga gaggccgtcc tcatcgctga
     4861 cgatgaacat ctcgtcgcgc tcagggtcgt agtagccgag cacctgttcg ctgagcagat
     4921 cgatctggaa ctggtagagg tcgagcgtcg gatcgtcgat cagccgtaac agccagagct
     4981 cctgagtgtc ctcatcggcc tcctcctgcg agtagtcggc attgacgtcg tcgatcaggt
     5041 tctggcgtaa ctgctcgcgg gagatgacgc tgatcggcac cgttttgagc agatccagct
     5101 tgcggatctt ctcgatccgc tgctcgattt cgtcaatctc ctcagccagc ttgggatcga
     5161 gtccggcctg cgtcgcttcc ggcgacgcgc tctcgccggt tgctgtctcg gtcgggctgg
     5221 ctgccgccct cgtgctggtt gtcgtcggcg acgcggcagg cgtctcggtc gcctcagccg
     5281 tcgccgttgg gctggcagat tccgtcgcgg cggccgtgac ggtcgctgtc gggtcgtccg
     5341 agtcggagcc gccgatgctg caagcgctga gtagcataag acagacgagc ccaaaggttg
     5401 tgaatgcggc cttcatgcgt gtcccgtcca tcagctaccc gaccaccggc atcgcgcaag
     5461 gcggagctgc gacgccgcga caagcataga ggacgcggca aaccgccccc aatcgtccaa
     5521 aggtccgggg tccgatttca cggtcgccgg taggggcgag gcactgactc gccctgttcc
     5581 ccgcccgaca cgttcggtgg ggagccgggc gagtcagtgc ttcgccccta ccaggacgta
     5641 tccgcttgtt tcattggcaa caccgaacta gaacaccgcg atgatctcgc gcaggaacgc
     5701 gccgagcagg acgccgcagg ccagcagcag caaccgagag ccggtatcgg aatcgaaagg
     5761 tagatcgcgg tcgccggtgc tgatcttcgg gcgactggga gcgagaccat gcttccgcag
     5821 tgtgtcgcca gcggactggt tggcgtgctg gagtcgctcg aactcgtcga gcacgacctt
     5881 gaccgcctcg tggtcgaggt cggaccactt cggaaccggc cggtcggcgt cggcgaagtc
     5941 ttcgacccgc cggcgaagat agatgacata attacgagcg tgttcgcgca tgacgtgttg
     6001 ctccctggtt gcgtcgcccg cgatcggcgc attcgttggc cgtcagccag gtgacgaccc
     6061 ttccctccat ggcgttggga tgacagggca agcggggcgg gtccttgttc actgccagcc
     6121 tggcggcgtc atcacgtccc gttagacagg aggacgacag gcgggcggca gtggttcccc
     6181 tggctcgcgg cggcattcgg cggggtagac ccagacggtg aaggtcgccg tggcgtcgcc
     6241 gaccgttgcg gtcagttccc agcagccgga ttcgccgaag atcagcgatg accgccagag
     6301 gttgccctgg acgccgatct gctggatgtt gtcggcagcg tagccgagct tcatgccgct
     6361 gtcgagggcg tggccctcga tcgtgatgtc gccccattcg tcggggaacc agtccagcgt
     6421 ggccggctgg ttggcccaga gcagaccgtc agttgaacgg atgctgatgc cggtgccgtc
     6481 catccagtaa gccgggccgt agtttccgag gcggtcgacc gggtcgcccc agccggggcc
     6541 gcatgagtcc ggcgcatggt acggcagcac tgcgtcgcgc cgctcgatcg cctggcgcat
     6601 gtccgggcgg tccgtcagcg gcaggacctt gacgacaatc gtcatcgaca ggtctcggac
     6661 gccggcggtc agttcccagc agccggcctc ggggaacggc acgacgatgt tgttgatctc
     6721 cgaatccgac gtctgctgga catcgacagg atcggcagga ccgtccaggc gcttggccgt
     6781 gatagagaag acatcaccgg cctggaacgg ccaatccccg agcactggcg tgcctcccgt
     6841 gaaccagcgc gtctcggatt ctggcaggct cgggttgatg ccgtcgatcg agatcgggct
     6901 gatccagaat tcgttctcgc cgtaccattc cttgtggtac gggtccgaca ggaagcggct
     6961 gggaggcgat gggttggtcg gcgttaccgc gcagcccgag gcgaccggcg tcgccgccgt
     7021 tggggtgacg gtgacggaga caggcgcgcc gacgacaggc tcaggcgtgc ccgacgacgt
     7081 atcgcgcgac tcccagaacg cgaagccgcc gacgacgagc gccaggacag tgatgacgac
     7141 gatggcttcg tggaggccgc gttgtcgccg ccaggggatg tccttcatgc ccggcagggt
     7201 ctgctcgatg ctggtcaggt ggcgttgccg ctcgtcctgg gtcgggacga cgtcgcggta
     7261 ggcatcgcgc aggacgttgc cgagccgttc ctggtcgcgt tcacccggca tcgatcactc
     7321 ccatctcttc ggccagccgg tgcagggcgc ggttgacgcg catccgggcg gcgccttccg
     7381 agataccgag cacccgcgcg acatcccgaa acgacagttc ggcgacgtag cgcagcagca
     7441 cgatctcgcg ctggcgactc ggcaagcgct ggacggcgct acggacacgc tcgtggtcct
     7501 cggagcggat cagcgcgtcg agcagatcgt cgttgagcgc gctcagggtg cggcggctca
     7561 ggaagcgccg ttgctggacc cgcccccgcg catcgtctgc gattcgccgg ctcgcgatcg
     7621 tgaagagcca actggcgaag ctgcccttgt cgggatcgta gcggtgcagc ccttcgagcg
     7681 cgccgaccat cgtgtcgttg aggacgtcgt cggcgatcgt ccgtgatccc gttcggccgg
     7741 cggcgtagcg gaagatgcgg tcggcatagc ggacgtagag ctcgccgaag gcgtggcgtt
     7801 gccgcaatgc cagccgagcg atctgctcgt cggacatcgt cgagagttcc ggttccgcgc
     7861 tcacggcttc cttcacggct acccccttac acaatacatc gggggttggc gcggtttgtc
     7921 acgaaatagc gctgccggcg acaacgaaca cgttctgatg gcaagatcag cgcagcagat
     7981 cgaccttcat gaagcgagtg ggacgatgga cgagaagtca cgcgatgtcg ttgtgatcgg
     8041 cggggggcaa acgggactgg cggccggcta ctacctgcgg cggacggggc tgtcgttcgt
     8101 catcctcgat gccgaggtcg ggccgggtgg cgcctggcga cactgctggg agtcgttgcg
     8161 gctgttctca ccggcgcgct ggtcctcgct gccgggctac gtcatgccgg gcggcacgga
     8221 gtactatccc gaccgcagcg aggtgctggc ctacatggcc gagtacgagc ggcgctaccg
     8281 gctgccgatc gagcggcccg tccttgtcgg cgctgttcgg gatggcggtg agcaggtgat
     8341 cgtcgagacc gaccgtggcg tctggcaggc acgggccgtc gtcagcgcaa ccgggagctg
     8401 gcgggcgccg tacatccccg agatcgaggg tcgtgaccag tttcggggcg agcagatcca
     8461 ctcggccgag tacgagtcgc cgatggcgtt cgtgggccgg cgcgtgctgg tggttggcgg
     8521 tgcgaactcc ggtgcgcaga tcatggctga gctctcgctg gtggccgacg caacctgggt
     8581 gacgcggcgc gagccggtct tcctgcccga caacgtcgat ggccgcgtcc tcttcgagcg
     8641 ggcaaccgag cgctacctgg cggcgaagga gggccgccag ttgccgcccg aatccggctc
     8701 cggcaagttc ctcggcgacg tcgtcatgct gccgccggtc cgcgaggcgc gcgaccgcgg
     8761 cgacctctcc agcgttcgca tgttcagccg ctttacccgc gacggcgtcg tctggcagga
     8821 cggacacgga gagcggatcg acgcggtcat ctggtgcact ggcttccgga ccgccctcga
     8881 tcatctggca ccgctgggcg tcgtcgatgc ctcgggacag gtcgagacgg acggcacacg
     8941 ctcggtccgc gatccacgtc tctggctgct gggctacggc gagtggaccg gctacgcctc
     9001 ggcgacgctg atcgggatcg gtcgttcggc gcgggcgacg gcggaggaga ttcttggcgt
     9061 gttgagtgct gcgtgaaggt ggcgcgatgg aatacgcgat agaccggtgc gagcgggata
     9121 tcctggctgg gctgacggcg accggactcg tgccggaaga ggcgctgacg gtcgaggagc
     9181 cgaaggcagg gattgcggcc gatctgacgt ttgctgcctt cggggtggcg cgggagcttg
     9241 gcgtgccgcc gccacagctg gcaactgaga tcgctgaacg gctgcggttc gagccgggtt
     9301 cgctggccgg gacggctaca gccgccgggc cgtacgtcaa tgtcagcgtc gacccggagc
     9361 gctttaccgg agcggtgctg gaggatatcg cgcgcggcgg cgatcgctac ggccacgacg
     9421 acgtcggggc gggtcagtcg atcgtcattg attactcgtc gccgaacatc gccaagcgga
     9481 tgcacgccgg tcatatccgt tcgacgatca tcggccaggc actggtcaac atgctctccg
     9541 cgctcggcta ccgggtcatc cgtgacaacc acatcggcga ctggggcaag agtttcggcg
     9601 tcctgctggc cgcgatcgag cgcgagggct atccggctgg cgacggggag ttgctgctgg
     9661 cggcgcttga ggacctgtat gccctccaga gtgcgctgat cgcccaggac ccggagcagg
     9721 atgcccgagc ccgcgagtgg tcgctgcggc tggagcgggg cgatccggag gcgcggcgca
     9781 tctggcagga gatcgtcgat ctgacgctgg cgatcaacca gaccagctac gaccggctcg
     9841 gtgtctcgtt cgaccacgtc cacggcgaga gcttctacga gccgatgctg gcagaggtca
     9901 tcgacgacgc gctcgccagc ggggccgcca ctcgcgaccc ctccggcgcg gtcgttgtcg
     9961 acgccggtga agggctgccg accttcctgc tgcaacgcag tgacggcggc acgctctacc
    10021 acacccgcga cgtggcgacg gcccgttacc gcgtccacga gttccagccg gccaaggtcc
    10081 tctacgtcat cggcgcgccc caggagctgt atctgcgcca gctcttc
//
LOCUS       DASUGJ010000051         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_700602, whole genome shotgun sequence.
ACCESSION   DASUGJ010000051 DASUGJ010000000
VERSION     DASUGJ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700602"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..710
                     /locus_tag="VFV93_01305"
     CDS             201..710
                     /locus_tag="VFV93_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164004.1"
                     /translation="MVEERWRLMRAFWAALVVSGVMGVAGIVSLVAAMADYNSTLETY
                     TQLSIGYEPESFAWVEPDFGAATTRIAIVNDSPADAVVENLNLYLRFDGEFAGTNYER
                     FAPVEVPKGETRVVTLNLEVSTPSIQPEGGTAQLSLDGWATVHFDGVERELRLKVDEP
                     IGVVPWEGP"
     gene            712..1242
                     /locus_tag="VFV93_01310"
     CDS             712..1242
                     /locus_tag="VFV93_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164005.1"
                     /translation="MRDAPPRSRRRSQISYWLLIAAQWLLLTFAIAGGMVRGSKALRD
                     YVSPSVGDGWLTGGALVAGLLLGLTVISPRVLVPLVISMCVVAAIVFGLIIYTPAWQG
                     TLFPSTRLSNYAQQQALFLGLWTTVPAIIGALIGYLLAGSIRQALETRRDGADTEHLP
                     WWERPSRSAAGDNTNS"
     gene            1402..>2087
                     /locus_tag="VFV93_01315"
     CDS             1402..>2087
                     /locus_tag="VFV93_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX5164006.1"
                     /translation="MLSLMTACGGSASDPEPTGTAQPAATATDPAPTTADTAAPAETA
                     STAPATAASQAVDWTPQPGLALLEPESGAYFGVNLDWGNDSAAAFNERLGVDAAAYVQ
                     FAPFPFDDGWRENLRLYFEQVLAVRGIAVLTLEPTIPLDEITAAQAEDLAQLLAGWNA
                     EGVGVLLRFAHEMNGSWYGWGQQPEAYVAAFRRVADAVHAMAPGTAMLWAPNYGAGYP
                     FSGGAYEAKPA"
BASE COUNT          313 a          654 c          741 g          379 t
ORIGIN      
        1 cacctcacgc ctccaggcgc tcgtcacagc cgtccgcaac ggcatcgtcg agatcgcctg
       61 acaagccgcc cccgtccttg tcatcctgag gcgtgagccg agggatctct cctcggttga
      121 ctgatcggct ggttgaggag agatccttcg gctcacgcat caggatgaca agcgggcagc
      181 tatactgcgg ggcaggccgg ttggttgaag agcggtggcg cttgatgcgg gcgttctggg
      241 cggcgctggt ggtctctggg gtgatggggg tggccgggat cgtctcgctg gtcgcggcga
      301 tggccgacta caactcgacg ctggagacct acacgcagct ctcgatcggc tacgagccgg
      361 agtcgttcgc gtgggtcgag ccggacttcg gcgcggcgac gacccggata gcgatcgtca
      421 acgactcgcc ggccgacgcg gtcgttgaga acctgaacct ctatctgcgc ttcgacggcg
      481 agtttgccgg caccaactac gagcgcttcg cgccagtcga ggtgccgaag ggggagacgc
      541 gggttgtgac gctgaacctg gaggtctcga cgcccagcat ccagccggag ggcgggacgg
      601 cccaactctc gctcgacggc tgggcgacgg tccacttcga cggagtcgag cgcgagctgc
      661 ggctgaaggt cgacgagccc atcggcgtcg tgccgtggga ggggccgtag gatgcgcgac
      721 gcgcctcctc gctcgcggcg acgttcccag atcagttact ggctgttgat cgcggctcag
      781 tggctgttgc tcacgtttgc gatagctggt gggatggtgc gcggctcgaa ggcgctgcgc
      841 gattacgtct caccgagtgt gggcgatggc tggctgacgg gcggcgcgct ggtggcgggg
      901 ctgctgctgg gactgacggt catctcgccg cgcgtgctcg tccccttggt aatttccatg
      961 tgcgtggtcg cggcgatcgt gtttgggttg atcatctaca cgccggcctg gcaggggacg
     1021 ctcttcccca gcacgcggct gtcgaactac gcccagcagc aggcgctgtt cctggggctc
     1081 tggacgactg ttccggccat catcggcgcg ttgatcggct acctgctggc cggcagcatc
     1141 cgccaggccc tcgaaacccg ccgcgacggg gccgacaccg agcatctgcc ctggtgggaa
     1201 cggccgagcc gcagcgccgc cggcgataac acgaattcct gatggttcct gtggcgcaat
     1261 tcgggactga tttctcttgc tgcatgcgtc tcccctgaac gacgatcgag cacctacgtc
     1321 gtgctcgaat cgcgcttgag ggagaccatg tcgcgccaat cacatcgtcg cagatacacc
     1381 gtactcgttg gacttttgat cgtgctgtcg ttgatgacgg cctgcggcgg ctctgcgtcc
     1441 gatccggagc cgaccggtac cgcccagccg gccgcgacgg cgacagatcc ggcgccaacg
     1501 accgcggata cggctgcccc ggccgagacg gcttcgacgg caccagcgac cgccgcaagc
     1561 caggcggtgg actggacgcc gcagccgggc ctggcgctgc tggagcctga gagtggcgcc
     1621 tacttcggcg tcaatctgga ctggggcaac gattcagccg ccgccttcaa cgagcgcctg
     1681 ggcgtcgacg cggcggccta tgtgcagttc gcgccgttcc cgtttgatga cggctggcgc
     1741 gagaacctgc gcctctactt cgagcaggtg ctggcagtac ggggaatcgc cgtcctgacg
     1801 ctcgaaccga cgattccgct ggatgagatc acagccgccc aggctgagga tctggcccag
     1861 ttgctagcgg gctggaacgc cgagggtgtt ggcgttctgt tgcgtttcgc gcacgagatg
     1921 aacgggtcgt ggtacggctg gggccagcag ccggaggcgt acgttgcggc gttccggcgg
     1981 gtagcggatg cggttcatgc gatggcgccg gggacagcga tgctctgggc accgaactat
     2041 ggcgcgggct acccgttctc gggcggcgcc tacgaggcga aaccggc
//
LOCUS       DASUGJ010000052         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_422939, whole genome shotgun sequence.
ACCESSION   DASUGJ010000052 DASUGJ010000000
VERSION     DASUGJ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422939"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFV93_01320"
     CDS             complement(<1..426)
                     /locus_tag="VFV93_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164007.1"
                     /translation="MCDTFDWVKPSPLWQLEAQDFRQAGFFRPQLLEFRTDAFMEDFF
                     AVAGDPKPAAIGAVVTPQPGETAALKLYQPVHGRFYLACASLCCRVPGFPDREVRTAD
                     GESTFFVLRKLLGGEEFGWVVSGTQKGWKPLNGQPRRVLA"
     gene            complement(448..1146)
                     /locus_tag="VFV93_01325"
     CDS             complement(448..1146)
                     /locus_tag="VFV93_01325"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX5164008.1"
                     /translation="MSTVLDQLEIAVERKRLYGAYSAQVTDIRDPDGQGRVKVKLPWS
                     PDLNGGGYEVWARLAVPMAGNNRGTWLVPDPNDEVLIMFEGGDPRLPFVIGALWNGQD
                     KPPETMDGAGKNVKKTIRSRNGVKITLDDTDGQETLTLETPGGQKVTLKDGPGTIEIQ
                     DANGNSARMEAAGVTLTAAAKLTIRAATVEVNAGMVTVNAAISKFSGVVQADTVITNS
                     IISASYSPGAGNIW"
     gene            complement(1152..>2201)
                     /locus_tag="VFV93_01330"
     CDS             complement(1152..>2201)
                     /locus_tag="VFV93_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164009.1"
                     /translation="SRPTIRIDGTIQAALGDVLLQSLLVEETTLGLFRCEANFLNWGP
                     KNNEVGFLFFDRQLLDFGKTFSVEFGPVGSAGPVFAGRISGIEAQYPPHRPPELTILA
                     EDRFQDLRMERRTRSFEDISDADVMRKIASQHGLTAQLDVNGPTHRTLAQVNQSDLAF
                     LRERAAAIDAELWMDDKTLYAQARARRNSGTATFTYGQNLIEFTVLADLAHQRTSVQV
                     NGWNVGAKAPIGEKAGESMIAAELNGGRSGSAVLATALKPRDEYVALAVPLSQPEAKA
                     MAEARYRARARSFLRGSGVVDGNVKLRVGSFIEFGGLGPFFDGRYYVTVARHTFNLRD
                     GYRTTFDVERPGIGG"
BASE COUNT          337 a          789 c          700 g          375 t
ORIGIN      
        1 cgccagcacc ctccgcggct ggccgttcag cggcttccag cccttctgcg ttcccgagac
       61 gacccagccg aactcctcgc cgcccagcag cttgcgcagg acgaagaagg tgctctcgcc
      121 gtcggccgtt cgcacctcgc ggtccgggaa gcccggcacc cggcagcaga gcgaggcgca
      181 ggccagatag aagcgcccgt ggaccggctg gtagagcttg agcgctgccg tctcgcccgg
      241 ctgcggcgtg acgacagccc cgatcgccgc cggcttcggg tcgccggcga cggcgaagaa
      301 gtcctccatg aacgcgtctg tgcggaactc cagcagctgc ggccggaaga agccggcctg
      361 gcggaagtcc tgcgcctcca gctgccagag tggcgatggc ttgacccaat cgaacgtgtc
      421 acacatccaa cggctcgctc tcctgcccta ccagatgttt ccggcgccgg gcgagtagct
      481 ggcgctgata attgagttgg tgatgaccgt gtcagcctgg acgacgccac tgaacttgga
      541 gattgcggcg ttgacggtca ccatcccggc gttgacctcg accgtcgccg cccggatcgt
      601 cagcttggcg gccgccgtca gcgtgacgcc ggccgcctcc atccgggccg aattgccgtt
      661 cgcgtcctga atctcaatcg tgccgggccc gtccttcagc gtcaccttct ggccgccggg
      721 tgtctcaagc gtcagcgtct cctgaccgtc cgtgtcgtcg agcgtgatct tgacgccgtt
      781 gcgcgagcgg atcgtcttct tgacgttctt gccggcgccg tccatcgtct cgggcggctt
      841 gtcctgcccg ttccagagcg cgccgatcac gaacggcaac cgggggtcgc cgccctcgaa
      901 catgatcagc acctcgtcgt tcgggtcggg caccagccac gtcccccggt tgttgccggc
      961 catcgggacc gccagccgcg cccagacctc gtagccgccg ccgttcaggt ccggcgacca
     1021 gggcagcttg accttcactc gcccctgccc gtccgggtcg cggatatcgg tcacctgggc
     1081 gctgtatgcg ccgtagagcc gcttacgctc gacggcgatt tcaagctggt caaggacggt
     1141 gctcatcgcg gctagcctcc gatgcccggg cgttcgacat cgaacgtcgt ccggtagccg
     1201 tcgcgcaaat tgaaggtgtg tcgcgcgacc gtcacgtagt agcggccatc gaagaacgga
     1261 ccgaggcccc cgaactcgat gaatgaaccg acccgcagct tgacgttgcc gtcaacgacg
     1321 ccggagccac gcaggaacga gcgcgcccgc gcccggtagc gcgcctcggc catcgccttc
     1381 gcctccggct gcgagagcgg caccgccagc gcgacgtact cgtcacgggg cttgagggcc
     1441 gtcgccagga cagcgctgcc gctgcggcca ccgttcagct cggcggcgat catgctctcg
     1501 ccagccttct caccgattgg cgccttcgcc ccgacgttcc agccgttgac ctggaccgac
     1561 gtgcgctggt gggccagatc ggcaaggacg gtgaattcga tcaggttctg accgtaggtg
     1621 aaggtcgccg tcccgctgtt gcgccgcgcg cgggcctggg cgtagagcgt cttgtcgtcc
     1681 atccacagct cggcgtcgat ggccgccgcc cgctcgcgga ggaaggccag gtcactctgg
     1741 ttcacctgcg ccagcgtccg gtgggtcggg ccattcacgt caagctgggc cgtcagcccg
     1801 tgttgcgagg cgatcttgcg catcacgtcc gcatcgctga tgtcctcgaa cgaacgggtc
     1861 cgtcgctcca tccgcagatc ctggaagcgg tcctcggcca gaatcgtcag ctccggtggg
     1921 cggtgcggcg ggtactgcgc ctcgatcccg ctgatccggc cggcgaagac cggcccagcc
     1981 gagccgaccg gcccgaactc gaccgagaac gtcttgccga agtcgagcaa ctgccggtcg
     2041 aagaacagga acccgacctc attgttcttc ggtccccagt tcaggaagtt cgcttcgcag
     2101 cggaacaacc ccagcgtcgt ctcctcaacc agcaacgact ggagcagcac atcgcccaac
     2161 gccgcctgga tcgtcccatc gatccggatc gtcggccgcg a
//
LOCUS       DASUGJ010000053         5680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1813212, whole genome shotgun sequence.
ACCESSION   DASUGJ010000053 DASUGJ010000000
VERSION     DASUGJ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813212"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1314
                     /locus_tag="VFV93_01335"
     CDS             82..1314
                     /locus_tag="VFV93_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164010.1"
                     /translation="MSRRAAGWLAWGILAVFGACIGAGLVLQYARDEPPGAFVTYLSL
                     AVAFGLFAVMGLLIVTRRPDNVMGWLYCAIGLGTAYTYVSPSYRLYTVEQPARDFPLV
                     AITSLLESVVWSLNLMLIIVFALLLFPTGRPLSRRWSIVGWIAGIGLTLSVLTTFLTG
                     GQILGIGETIDNPIGIEAFRPVGMLLYDVADILMLVSCPLAIAAIIVRFRRARGVERQ
                     QIKWLAFAATGGFVLAAGNALLGSPLGDVGFAIAIAMIPLMTGLAILRYRLFDIDRLI
                     NRALVYSLLSGVLALVYLAAIGVFDVALRGMSGQSSDLATAASTLLVAGLFRPVRGRI
                     QSTVDQRFYRRAYDAARVLEQFGQRLREEVDLPTLDLALRDAIASTMQPAHVSLWLRP
                     VDTDFRGRSAGHEAEISR"
     gene            complement(1304..1792)
                     /locus_tag="VFV93_01340"
     CDS             complement(1304..1792)
                     /locus_tag="VFV93_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164011.1"
                     /translation="MQASQITPYLPRALVYGAVGAAFAALTIGIPTDVIPNGFFTRMT
                     PVRVQDYVFLIVTAILAGILAASYALPQTRACSIEQGRATLGGLLSFLAIGCPTCNKV
                     IVLLLGTSGALNIFEPLQPLLALASFALLVLAIWIRWRPVVQQAAAVGVGEQARPAGF
                     ND"
     gene            complement(1967..3466)
                     /locus_tag="VFV93_01345"
     CDS             complement(1967..3466)
                     /locus_tag="VFV93_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX5164012.1"
                     /translation="MSDTLTKAQMNRRTLLKQAGIFGLGLPAAAMLINACGTDEDPDA
                     TRTVNTGGLVVTPPAASPVAEHGHAQPAAATAELTITAQDLKFTPTTLSAEAGQLVRL
                     TFVNEGAIEHDWYAEGMPVDGLTAVEQPAGFSARMAGIWDTATGAGNPYAGAGTGEQM
                     VVEFTPSEAGEFQFICAVPGHAQAGMTGTFTVSAAGDTAAATPESTEVDADEMDRMHE
                     AGVKAFPAPTEGLGGQPLEYTLDGDVKVFDLTCSVIEWEVEPGKKYEAWAYNGVVPGP
                     EIRVTEGDKVRINVTNTLPQSTGVHWHGLIVPNSMDGVPFITQPPIKPGETFTYEFQI
                     REGNAGSHMYHSHHNAAFQVTMGLLGAFIVEPKDPSTRPAFDREYTLILNDGPLGGFT
                     INGKGFPATQPLTAKVGEKIMIRYMNEGLMIHPMHLHGMPQLVIAKDGWLQPQPWLCD
                     TLNVAPGERWEVLVDVTEPGVWAFHCHILSHAESDHGMFGMVTALIVEE"
     gene            complement(3513..4130)
                     /locus_tag="VFV93_01350"
     CDS             complement(3513..4130)
                     /locus_tag="VFV93_01350"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5164013.1"
                     /translation="MDGNREKRSGRQADVIQAPPVAQVLFGNSRLAWLWLVARVYLGW
                     LWLDAGRQKLAEDGWMDGGLALRGIWERSIQTPEQEQAPLAYSWYHDMLQFMLDHKWY
                     VWAGPLVAVGETLLGIALILGFFTGLTAFLGSLLNAGIVLTGAAANPFFFVLAIALML
                     AWRTAGLIGLDRWVLPLSGKIWSGDIGPPDRSGANQRGHSARAAG"
     gene            complement(4324..4767)
                     /locus_tag="VFV93_01355"
     CDS             complement(4324..4767)
                     /locus_tag="VFV93_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164014.1"
                     /translation="MDPVSVRLVRASLLWLVLGFTLGALMLSDALVPGDWRAWFAPTH
                     GHILFVGWFLQFAIGVGYWLLPRKRTPERPLGYNERHATIAFGFLNSGLLLRVLAEPP
                     GRVGNGGDLVDITLAVSSMLQLAAIVIIASQIWPRIVPRRVARAE"
     gene            complement(4775..>5680)
                     /locus_tag="VFV93_01360"
     CDS             complement(4775..>5680)
                     /locus_tag="VFV93_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164015.1"
                     /translation="SLIRLFTSEPVTKVAPPLPFAEQAAIDRIGISFTRMAGMYLLLG
                     LAVGLMTTFWTPDRGRWDLVWAHAMLLGWFLQMVAGVCYHVLSRWTGARWQSPQRIRW
                     HLLAVTLGLPAMVAALALDLDWLFAVAGPLQAVALGLFLWNIAPLVLRMPSLSRLGML
                     LAAGFLLVGITIGASAALDPANGVRLRFSHATINLLGFAGLLICGVGYYLFPRLAGQA
                     LRWPRLATIQLAAHASGVALVATAWWYQLAVDDGAQPLITAGALLVAAGFAVFGGIVA
                     ATFQGSGRGVVAGVSLQPRRSPQGR"
BASE COUNT          891 a         1892 c         1920 g          977 t
ORIGIN      
        1 atgtcgcggg cagtcggtcg tcgacctcgc gaggtgtgtc gtgtgcgtgt ctgcggcgcc
       61 agcctggaag gtggggttac gatgagtcgt cgagctgctg ggtggctggc ctggggcatt
      121 cttgcggtgt tcggtgcctg cattggggca ggactggttc tacagtacgc acgtgacgag
      181 ccgccgggtg cgttcgtcac ctacctctcg ctcgcggtcg ccttcggcct gttcgcagtg
      241 atgggtctgc tgatcgtcac acgccggccc gacaacgtca tgggctggtt gtactgcgcg
      301 attgggctcg gcaccgccta cacgtatgtc agccccagct atcgcctcta tacggtggaa
      361 caacccgcgc gtgacttccc gctcgtggcg atcacatcgc tcctcgaaag tgtcgtctgg
      421 tcgctgaacc tgatgctgat catcgtcttc gcgttgctgc tgttcccaac cggccggccg
      481 ttgtcacgac gctggtcgat cgttggctgg atcgccggga ttgggctgac gctgtcggtt
      541 ctcacgacgt tcttgaccgg tggccagatt ctcggtatcg gcgagacgat cgacaacccg
      601 atcgggatcg aagcgttccg gccggtgggc atgctgctgt acgatgtcgc cgacatcctg
      661 atgctggttt cgtgcccgct tgccatcgcc gcgatcatcg tgcgatttcg ccgtgcgcgg
      721 ggtgtcgagc gacagcagat caagtggctc gcgttcgcgg cgaccggcgg gttcgtgctg
      781 gccgccggta acgcgctcct gggatcgccg ctcggcgatg tgggattcgc catcgcgatc
      841 gcgatgattc cgctcatgac cggtctggcg atcctgcgct accggctctt cgatatcgac
      901 cgcctcatca atcgcgcgct ggtctacagt ctgctctccg gtgtcctcgc cctggtctac
      961 ctggctgcga tcggcgtctt cgatgtggcg ttgcgtggca tgtcgggcca gtcgtccgac
     1021 ctggcgacgg ccgcttcgac gctgctcgtc gccggcctgt tccggcccgt tcgcggtcgc
     1081 atccagtcga cggttgacca acgcttctat cgtcgcgcct acgacgcggc ccgggtgctc
     1141 gaacagttcg gtcagcgctt gcgtgaagag gttgacctgc cgacgctcga cctcgccctg
     1201 cgcgacgcca tcgcctcgac gatgcaaccg gcgcacgtct cgctctggct acgtccggtt
     1261 gatacggatt tcagagggcg atcagccggg catgaggcgg agatcagtcg ttgaacccgg
     1321 ccggccttgc ctgttcaccg acgccaacgg ctgccgcctg ctggacgacc ggccgccaac
     1381 ggatccagat cgcgagcacg agcagcgcga acgaggccag cgccagcagc ggctgaagcg
     1441 gctcgaagat gttgagggcg ccgctcgtgc cgagcagcag gacgatgacc ttgttgcagg
     1501 tcgggcagcc gatcgccagg aacgagagca acccgcccag cgtcgcccgg ccctgctcga
     1561 tcgagcaggc acgcgtctgc ggcagcgcat acgacgccgc caggatgccc gccaggatcg
     1621 ccgtcacgat caggaagaca tagtcctgca cgcgcaccgg cgtcatccgg gtgaagaacc
     1681 cgttcgggat gacatcagtc ggaatcccga tcgtcagcgc cgcgaaggca gcaccaaccg
     1741 cgccgtagac gagcgcgcgt ggcagatatg gcgtgatctg gctggcttgc atcaaacgac
     1801 cctccccggc tgcgacaccg cccattgtgt cgcaactggg gagggaatgc cagttaatat
     1861 gggaaccggg attcgggatt cgggggtggg tgacgttcga tgttcctgaa catgcgctcg
     1921 gctctccagc cccgaatccc gaatcccggt tcccgactcc ccgtccctac tcctccacaa
     1981 tcaacgccgt caccatcccg aacatgccgt ggtcagattc ggcgtgggag aggatgtggc
     2041 agtggaaggc ccagacgcct ggctcggtca cgtcgaccag cacctcccag cgctcgccgg
     2101 gggcgacgtt gagcgtgtcg cacagccacg gttgcggctg gagccagccg tccttggcga
     2161 tgaccagttg cggcatcccg tgcaggtgca tcgggtggat catcagcccc tcgttcatgt
     2221 agcggatcat gatcttctcg ccgaccttcg ccgtcaatgg ctgggtggcc gggaagccct
     2281 taccgttgat cgtgaagccg cccagcgggc cgtcgttgag gatcagcgtg tactcgcggt
     2341 cgaaagccgg ccgcgtcgaa ggatccttcg gctcgacgat gaacgcgccc agcagcccca
     2401 tcgtcacctg gaaggcggca ttgtggtgtg agtggtacat gtgcgagccc gcgttgccct
     2461 cgcggatctg gaactcgtag gtgaacgtct cgcccggctt gatcggcggc tgggtgatga
     2521 acggaacacc gtccatgctg ttggggacga tcaggccatg ccagtggacg ccagtgctct
     2581 gcggcagcgt gttggtgacg ttgatgcgga ccttgtcgcc ctcggtgacg cggatctccg
     2641 ggccggggac gacgccgttg taggcccacg cctcgtactt cttgcccggc tcgacctccc
     2701 actcgatcac cgagcaggtc aggtcgaaga ccttgacatc gccgtccagc gtgtattcga
     2761 gcggctggcc gccgagtccc tcggtcggcg ccgggaacgc cttgacgccc gcctcgtgca
     2821 tccggtccat ctcgtcggcg tcgacctcgg tcgattccgg cgtcgcggca gcggtgtcgc
     2881 cggcggcgct gacggtgaag gtgccggtca tcccggcctg ggcgtggccc gggacggcgc
     2941 agatgaactg gaactcaccg gcctcgctcg gcgtgaactc gacgaccatc tgctcgccgg
     3001 tgccggctcc ggcgtagggg ttacctgcgc ccgtcgccgt gtcccagatg ccggccatcc
     3061 gggcgctgaa tccggccggc tgctcgacgg cggtcagacc atcgaccggc atcccttcgg
     3121 cgtaccagtc gtgctcgatc gcgccctcgt tgacgaacgt cagccggacc agctggccgg
     3181 cttccgcgct cagcgtcgtt ggcgtgaact tcaggtcctg agcggtgatg gtcagttccg
     3241 ccgtcgctgc cgctggttgg gcgtggccgt gctcggcgac cggcgatgcg gccggcgggg
     3301 tgacgaccag cccgccggtg ttgacggttc gggttgcgtc ggggtcctca tccgtcccgc
     3361 aggcgttgat cagcatcgcc gctgccggca ggccgaggcc gaagataccg gcctgcttca
     3421 gcagcgtgcg ccggttcatc tgtgctttcg tgagcgtgtc tgacatctga ggttccctgt
     3481 ctccttctct cgatctcgtg acggtcacat gctcagcccg ccgcccgggc agaatggccg
     3541 cgctggttgg cgccggagcg gtcaggcggg ccgatatccc cgctccagat cttcccgctg
     3601 aggggcagga cccagcggtc gagtccgatc aggccggccg ttcgccaggc cagcatcagc
     3661 gcgatggcga ggacgaagaa gaaggggttg gctgccgccc cggtcaggac gatgccggcg
     3721 ttcagaaggc tgccgaggaa ggctgtcagg ccagtgaaga aaccgaggat cagcgcgatc
     3781 cccagcagcg tttcaccgac cgcgaccagc ggcccggccc agacgtacca cttgtggtcg
     3841 agcatgaact ggagcatgtc gtggtaccag ctgtaggcca gcggcgcctg ctcctgctcc
     3901 ggagtctgga tcgaacgctc ccagatgccc cgcagcgcca gcccgccgtc catccagcca
     3961 tcttccgcga gcttctgtcg cccggcatcc agccagagcc agccgaggta gacccgcgcc
     4021 accagccaga gccaggccag ccgcgagttg ccgaacagca cctgcgcgac tggtggtgcc
     4081 tgaatgacgt cggcctgtcg tcccgatcgc ttctccctgt tgccgtccat cgtgtcccgc
     4141 ttttcgctgc gccacgcatg tactggtgta atcgtgcgct ttgcagcctg cagttggaca
     4201 cgaccggcag ggtggttcac tgcctacccg aatggatggt gtgggggctg agtgctgagt
     4261 gctgaggagt tcccgcgtag gggcgagggc cgcagcctcg ccccgcgaac cactcagcac
     4321 tttctactca gcacgcgcca cccgtcttgg cacaatccgc ggccagatct ggctggcgat
     4381 gatgacgatg gcggccagtt ggagcatgga ggagacggcc agcgtgatgt cgacgaggtc
     4441 gcccccattg ccgacgcggc cgggtggttc ggcgaggaca cgcagcagca ggccgctgtt
     4501 gaggaagccg aaagcgatcg tcgcgtgccg ctcgttgtag cccagcggcc gctccggtgt
     4561 tcgcttgcgg ggcagcagcc agtagccgac gccgatcgcg aattgcagga accagccgac
     4621 gaagaggatg tggccgtggg tcggcgcgaa ccatgcccgc cagtcgcccg gcacgagcgc
     4681 gtccgagagc atcagcgcac cgagcgtgaa gcccagcacc agccagagca gcgacgcgcg
     4741 caccagccgg acgctgacag ggtccatctc gccgctatcg tccctgcggg ctgcggcgcg
     4801 gctggaggct gacaccggcg acgacgcccc ggcccgagcc ctggaaggtt gcggcgacga
     4861 tcccgccgaa gacggcgaat ccggccgcga ccagcaatgc gccggccgtg atcagcggct
     4921 gtgcgccgtc atcgacggcc agctggtacc accaggccgt cgccaccagc gcgaccccgc
     4981 tcgcgtgggc cgcaagttgg atcgtcgcca gccggggcca acgtagtgcc tgaccggcca
     5041 ggcgtgggaa gaggtagtag ccgacgccac agatcagcag gccggcgaag cccagcaggt
     5101 tgatcgtcgc gtggctgaag cgcagccgga cgccgttggc cgggtcaagc gccgccgacg
     5161 cgccgatcgt gatcccgacg agcaggaagc cggccgccag cagcatcccc agccgtgaga
     5221 gcgacggcat cctgaggacc agcggcgcga tgttccagag gaacaggccg agcgcgacgg
     5281 cctgcaacgg gccggcgact gcgaagagcc agtcgaggtc gagcgccagc gcggccacca
     5341 tcgccggcag cccaagcgta acggccagca ggtgccagcg gatgcgttgc ggcgactgcc
     5401 agcgggcgcc ggtccagcgc gagaggacgt ggtagcagac gccggcgacc atctgcagga
     5461 accagcccag cagcatcgcg tgcgcccaga ccaggtccca gcggccccgg tcgggcgtcc
     5521 agaacgtcgt catcagcccg acggccagac ccagcagcag atacattccg gccatccggg
     5581 tgaagctgat gccgatcctg tcgatagccg cctgctcggc gaagggcagc ggtggggcga
     5641 ccttcgttac cggctccgac gtgaaaaggc ggatcaggct
//
LOCUS       DASUGJ010000054         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1535638, whole genome shotgun sequence.
ACCESSION   DASUGJ010000054 DASUGJ010000000
VERSION     DASUGJ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535638"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFV93_01365"
     CDS             <1..856
                     /locus_tag="VFV93_01365"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164016.1"
                     /translation="NGTIMAVIQMVRQTLVPALVSREHMANAVSLNSAGSNAMRIIGP
                     SIAGLIIGAFGAGVCFVLQALALVGALMTTTRIEPLENQRTGIAAGGILDGWYEVRKR
                     PALGSLILITAIPSLLVFPYIQMMPVFARDVFDIGAGGLGLLMAASGVGALTGALTAA
                     TMDRVARKGRMVMLLTVVYCLAVCGFAISPHPALAMFGLLISGITGSIFGSLTSTLVL
                     LLADARIRGRVMAIYTLTNGFTPFGALVLGAAAEAFGAPPAVGIACAIAALLTSICVF
                     FMNSLRET"
     gene            892..1509
                     /locus_tag="VFV93_01370"
     CDS             892..1509
                     /locus_tag="VFV93_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164017.1"
                     /translation="MSTQQSGYLPLSAEARRLYQVLLRTVADDGPELSSGAFPDISDT
                     RGGGNLSDFGALVDAGLIAHDVETGLVTAVLPLVAREPATVVYLDDDDQLPRLTVGVV
                     DGFALAVLLDRRVRIIDRCPTCGELVSIMAGPDGIRGRDPRSAVAVRIPADGPRLSSA
                     DTIRLTCSPEHGQAALEQSGNPDAVLQSIEGLAIEARERYAVVLP"
     gene            1552..>2114
                     /locus_tag="VFV93_01375"
     CDS             1552..>2114
                     /locus_tag="VFV93_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5164018.1"
                     /translation="MTATSTDLYYPAAGDGWQRRDPTDVGFDAAKLAEAVAFIEAQGH
                     DLEHEQRVSLDLPDNETVGPIKPKGGVNGLVVRNGYIAAEFGDTPSIEMTYSVAKSYL
                     STVAGLAFDRGMIPDLDQPVGKTLDAPEFQGKHNGKITWRHLLQQTSEWTGTLWGMRD
                     LADRRKGVDREIQEPGTFYEYNDVRVNL"
BASE COUNT          339 a          720 c          692 g          363 t
ORIGIN      
        1 gaacgggacg atcatggccg tcatccagat ggtgcggcag acgctggtgc cggcactcgt
       61 ctcgcgcgag cacatggcaa acgccgtctc gcttaactcg gccggcagca acgcgatgcg
      121 catcatcggc ccctcgatcg ccgggctcat catcggcgcc tttggcgccg gcgtctgctt
      181 cgtccttcag gcgctggcgc tggtcggcgc gctgatgacg acgacgcgga tcgagccgct
      241 cgaaaaccag cgcaccggga tcgcagccgg cggcatcctc gatggctggt acgaggtgcg
      301 caagcggccc gcgctcggct cgctgatcct gatcacggcg atcccgtcgc tgctcgtctt
      361 tccctatatc cagatgatgc cggtcttcgc ccgcgatgtg ttcgacatcg gagccggcgg
      421 gctcgggttg ctgatggcgg ccagcggggt cggcgcgctg accggcgcac tgacggcggc
      481 gacgatggac cgcgtcgcgc gtaagggccg gatggtgatg ctgctgacgg tcgtctactg
      541 cctggccgtc tgcggcttcg cgatcagccc acacccggcc ctggcgatgt tcgggctact
      601 gatttccggc atcaccggct cgatcttcgg ctcgctgacc agcacgctcg tcctgctgct
      661 ggccgatgcc cggatccgcg ggcgggtgat ggcgatttac acgttgacga atggcttcac
      721 cccgttcggc gcattggtgc tcggcgcggc agccgaggcg ttcggcgcgc cacccgccgt
      781 cggcatcgcc tgcgcgatcg cggcgctcct gacgagcatc tgcgtgttct tcatgaactc
      841 gctgcgtgaa acgtgacgat ctacgatccc ttcgacaaag gaggccagtc cgtgtcaact
      901 cagcaatccg gctatctgcc gctctcagcg gaggcgcgcc ggctctacca ggtcctctta
      961 cgaacggtcg ctgacgatgg ccccgagctg tcatccggcg ccttcccgga tatctccgat
     1021 acgcgcggcg gcggcaacct gtccgacttc ggcgcgctcg tcgatgccgg attgatcgcc
     1081 catgatgtcg aaaccggact ggtaacggcg gtcctgccgc tggtggcccg tgaacctgcc
     1141 accgtcgtct acctggatga cgatgaccag ctcccacgct tgacggtcgg cgtcgtcgat
     1201 gggttcgcgc tggcggtgtt gctcgatcga cgtgtacgca tcatcgaccg ttgcccgacc
     1261 tgtggggaac tggtttccat catggctggt cccgacggca ttcgaggtcg tgatccacgc
     1321 agcgccgttg ccgtccgcat tcccgccgat ggtcctcgcc tgagttccgc cgacacgatc
     1381 cggctgacct gttctcctga gcacggacag gcggcgctgg aacagagcgg caaccccgac
     1441 gccgtcctcc agtcgatcga ggggctggcc atcgaggcgc gcgaacgcta cgcggtcgtg
     1501 ctaccctgac ggcagccaat cagtacgaac gcagcgggag gaacgacgac gatgacagcg
     1561 accagcactg acctctacta tccggccgcc ggcgacggct ggcagcggcg ggatccgacc
     1621 gatgtgggat tcgacgccgc gaagctggcc gaggccgtcg ccttcatcga ggcgcagggc
     1681 cacgacctgg agcacgagca gcgcgtctcg ctcgacctgc cggacaacga gacggtcggg
     1741 ccgatcaagc cgaagggtgg cgtcaacggc ctcgtcgtcc gcaacggcta catcgcggcg
     1801 gagttcggcg acacgccctc gatcgagatg acctacagcg tcgccaagag ctacctctcg
     1861 acggtcgccg ggctggcgtt tgaccgcggg atgatccccg atctcgacca gccagtcggc
     1921 aagacgctcg acgcgcccga gttccagggc aagcacaacg ggaagatcac ctggcgacac
     1981 ctgttgcagc agaccagcga gtggaccggc accctctggg gcatgcgcga cctggccgac
     2041 cggcgcaaag gggtcgaccg cgagattcag gagcccggca ccttctacga gtacaacgac
     2101 gtgcgcgtca acct
//
LOCUS       DASUGJ010000055         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_146258, whole genome shotgun sequence.
ACCESSION   DASUGJ010000055 DASUGJ010000000
VERSION     DASUGJ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146258"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..2032
                     /locus_tag="VFV93_01380"
     CDS             392..2032
                     /locus_tag="VFV93_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX5164019.1"
                     /translation="MPRYFDDTGFWVQGPFREYWESHGGLYIFGYPITGVFDDNGLYK
                     QYFERAIFEYHPEHAGTEYEVLLQRLGAIRTEGRTAEEPFQPLDVEPDENCDVHDLTG
                     HRLCFGFRDYWNHNGGLSNFGYAISEEFQERNEPPPAGDGEEHTVQYFERTRFEWHPE
                     YQGTEYEVLLGLLGSEYLAAHGAPAEALARQPKDLPPADPTTGLRYGPHVGYGFNIVW
                     HGDDQSQAFHQQTLDKVNEAGFSWIRFQVGWRNAEPQPGQYRFDHIDRLVDLAKANNV
                     RIMASVLKAPSWATENGSDGLPVDAAAYGEFMRRLAEHFKGRIDAYEIWNEQNLAFET
                     GGMVDVGRYVNVLKAGYTAVKASDPNAIVVFGGLTPTGVNDPTIAIDDANYLSQFYAY
                     NDGEGKQYYDVLGTHPGSACNPPDTSWPDNPGCAEGWRDHNSHYFKRAEDIRRVMVEN
                     GEGAKQIWLTEFGWSSSPTPGPGQEFAAFNSEQEQADYIVRAFEIARNDWPWMGVMFL
                     WNLQFSVDDPSDKKGYWSVLNPDWSPRPAYTALKDMPK"
     gene            2169..>2443
                     /locus_tag="VFV93_01385"
     CDS             2169..>2443
                     /locus_tag="VFV93_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164020.1"
                     /translation="MKRLLRATILVLVLTMIVAACGGGGDDSDADPTATTADSAATTE
                     VPATETTEAAAATEPAGETPSTTETPVATSDQATATSPGAGATATESS"
BASE COUNT          469 a          770 c          782 g          422 t
ORIGIN      
        1 ggatcgtcgt agcgtctgcg caagcgcatc actagcagcg aaagcgtgct actgtctcgc
       61 tcgaatgtgc cgcccccgtt gtcatcctga ggcgtgagcc gaaggatctc ttctcgggta
      121 actgatcggc taacgcagga gagatccttc ggctgacgcc tcaggatgac aaccggggcg
      181 gtgtcgttcg atttggtacg ctcatgtcct caccggccgt gaaccggtcc gacaacatat
      241 ggggtgaacc gttcgaaggg gtcagatgct gtccagaaaa agcaggttgg ttgcgcgtgt
      301 cgcgctgttg cttgcgctca tcgtgagcgc ggccagtccg gcgcttacga gccaggtgaa
      361 cgcgcagtat gcaccgaaca acgatctccg gatgccgcgc tatttcgatg acacagggtt
      421 ctgggttcag ggtccatttc gcgaatactg ggagtcacac ggcgggctgt atatcttcgg
      481 ttacccgatc accggcgtct tcgacgacaa cgggctctac aaacagtatt tcgagcgagc
      541 aatcttcgag taccacccgg aacatgccgg aaccgagtac gaagtgctcc tccagcggct
      601 cggtgcgatt cgcaccgagg gccgcacggc cgaggagccg ttccagccgc tggatgtcga
      661 gcctgatgag aattgcgatg tccacgacct gactggccac cggctctgct tcggcttccg
      721 cgactactgg aaccacaatg gcggcctctc caacttcggc tacgcaatat ccgaggagtt
      781 ccaggagcgt aacgagccgc cgccggccgg cgacggcgag gaacacaccg tccagtattt
      841 cgagcggacc cgcttcgagt ggcatcctga gtatcagggt actgagtatg aagtgttgct
      901 cgggctgctc ggctcagagt acctcgccgc gcatggcgcg ccggccgagg cgctggcgcg
      961 ccagccgaag gatctgccgc cggccgaccc gacgaccggg ctgcgctacg gaccgcacgt
     1021 tggctacggc ttcaacatcg tctggcacgg cgacgaccag tcgcaggcct tccaccagca
     1081 gacgctcgac aaggtcaacg aggccggctt ctcgtggatt cgcttccagg tcggctggcg
     1141 caacgcggag ccgcagccgg gccagtaccg gtttgaccat atcgatcggc tggtcgatct
     1201 ggcgaaggcg aacaacgtgc ggatcatggc cagcgtcctg aaggcgccca gctgggcgac
     1261 cgagaacggt tcggacggcc tgcccgttga cgcggccgcc tatggtgagt tcatgcggcg
     1321 actggcggaa catttcaagg gccggatcga cgcctacgag atctggaacg agcagaatct
     1381 ggcgttcgag acgggcggga tggtcgatgt cggccgctac gtgaatgtgc tgaaggccgg
     1441 ctacacggcc gtcaaggctt ctgacccgaa cgcgatagtc gtcttcggcg gtctaacccc
     1501 gaccggcgtc aacgacccga cgatcgcgat cgacgacgcc aactacctca gtcagttcta
     1561 tgcctacaac gacggcgagg gcaagcagta ttacgacgtg ctcgggacac atcctggctc
     1621 tgcctgcaac ccgcccgaca caagctggcc ggacaacccc ggctgcgcgg aaggctggcg
     1681 cgaccataac tcgcactact tcaagcgggc cgaagacatc cggcgggtga tggtcgagaa
     1741 cggtgagggt gccaagcaga tctggttgac cgagttcggc tggtcctcgt caccaacgcc
     1801 tggacccggc caggagttcg cggccttcaa ttccgaacag gagcaggccg actacatcgt
     1861 ccgcgccttt gagatcgcgc ggaatgactg gccctggatg ggcgtcatgt tcctctggaa
     1921 cctgcagttc tcggtcgatg acccgagcga caagaagggc tactggagcg ttctcaaccc
     1981 cgactggtcg ccgcggccgg cctacacggc gctgaaggac atgccgaagt agcgcaattg
     2041 ctccgcacca cgcacaattg tacgtacacc ggctccgacg tctccgcttg cggggacgcc
     2101 gggccggcat gtcccgcccg gcatcggtgc gaccgttacg tcgccaacgg aggaaacgaa
     2161 gtcccagtat gaagcgtttg ctgagagcta ccattctcgt tctcgtgctg acgatgattg
     2221 ttgctgcctg cggcggcgga ggcgacgatt cggacgccga tcctaccgcg acgacggccg
     2281 attcggcggc gaccaccgag gtaccggcga cggagacgac cgaggctgcc gccgcgactg
     2341 aaccggcggg tgagacgccg tccaccaccg agacgcccgt agccacgagc gatcaggcga
     2401 cggcgacctc gccgggcgct ggcgcaacgg cgaccgaatc gtc
//
LOCUS       DASUGJ010000056         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_841061, whole genome shotgun sequence.
ACCESSION   DASUGJ010000056 DASUGJ010000000
VERSION     DASUGJ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841061"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..598)
                     /locus_tag="VFV93_01390"
     CDS             complement(110..598)
                     /locus_tag="VFV93_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5164021.1"
                     /translation="MEEARQQYIEDFGLLFEEFGGGRMVGRILGLLMVADAPELSAEE
                     IADTLKVSRGSISQSTRILIQLGMVRRTSRPGERRDYFQLRPDAWTEATRRRGHEIER
                     LIAIFERGARLTAGGDPRQRRQLEESLVFMQFWKQTLADMFPAWERERERRFGDTGNR
                     NP"
     gene            complement(702..1583)
                     /locus_tag="VFV93_01395"
     CDS             complement(702..1583)
                     /locus_tag="VFV93_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX5164022.1"
                     /translation="MKIVSLLPSTTEICYALGLGDALVGVTHECDFPVGALAKPKLTA
                     NVLPEGPHSSADIDRMISERVLSGQSIYHLDTGLLTELAPDLILTQELCEVCAVSYTD
                     VLAAAQALPKVPEVAAFEPHTIEEILASIGEVARLAGVRERGEEVVAALRSRLAAVAE
                     RTGDRPAPSVLCLEWLSPPMVGGHWVPEMVRLAGGVDVLGPEGQPSTYVTWEQIAVAD
                     SDVIIVMPCGYDLPATCVAMRELNDIPAWHTLRAVREGRVYPVDGSSYFNRPGPRVVD
                     GVELLARLIHQAAVPAS"
     gene            1904..2134
                     /locus_tag="VFV93_01400"
     CDS             1904..2134
                     /locus_tag="VFV93_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164023.1"
                     /translation="MSDEQRPAEDAPNPELVEAIVAQVRGLIQDERDEAALDAVRRTA
                     ERHLKMAAKLRAYPLRNADEPDFVFRPYRAEG"
     gene            2138..3538
                     /locus_tag="VFV93_01405"
     CDS             2138..3538
                     /locus_tag="VFV93_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5164024.1"
                     /translation="MAGEIYNQTIEDLGRKLRARETTPTELTRLYLERLDSVGRRYNA
                     VVTITEERALREAAQAEAELAAGYDRGPLHGIPYGAKDLLSVPGYPTTWGAVPYKDQS
                     FDEESQVTTKLRDAGAVLVAKLGMVELAGGMGYNQPNASLTGPGITPWNADEHPSWSG
                     GSSSGSGSAVASGAVGFAIGSETWGSITTPAGFCGLSGLRPTYGRVSRRGAMALSWTM
                     DKLGPMTRSAHDCGLVLNAIAGHDPGDPSSSQRPYSYPPAAERTTGFRFGLLSDATTN
                     VQDAVRDNFLASLRVLEEIGTVETVTLPDLPYNEVAGIIIQAELSSAFEELMTSGDVT
                     QLTAPEDRVNGLASLAMPANVYIRALRIRRHICVAMDELTSRFDALISPTLPTVASPV
                     DQSFEAYFSRANRSALGGAANAAGLPGIAIPNGYGERGLPTSLQFTGRAWDENTILAA
                     ARAYQSRTDWHTRWPC"
BASE COUNT          620 a         1259 c         1192 g          571 t
ORIGIN      
        1 cgtcgacttg ccggcgccgt tgggaccaag gaagccgaag atctcgccgc gctcgaccgt
       61 caagttcaca ttgcggattc cacggtcagc accgtaggtc ttggtcaggt catgggttgc
      121 gattgccggt gtcaccgaaa cgcctctccc gttcgcgctc ccaggccgga aacatgtcgg
      181 cgagggtctg tttccagaat tgcatgaaga cgagcgactc ctccagctgg cgtcgctggc
      241 gcggatcgcc gccagccgtg agccgtgcac cacgctcgaa gatggcgatc agacgttcga
      301 tctcgtggcc gcgccggcgg gtcgcctcgg tccaggcgtc gggacgaagc tggaagtagt
      361 cgcggcgctc gccgggccgg ctggtgcggc gcaccatccc gagctgaatc aggatcctcg
      421 tgctctggct gattgagcca cggctgacct tgagcgtgtc cgcaatctcc tccgccgaga
      481 gttcgggcgc atccgcaacc atcagcagcc cgagaatgcg gccaaccatc cggccgccgc
      541 cgaactcctc gaacaagagt ccgaagtcct cgatgtattg ctgacgcgct tcctccaccg
      601 gcgacgctcc cttccgcagt tcagtttaga actgtcagat gttatcgtca atactgaaac
      661 tagtgaagtt cggctgtcgt gcacgactgt cgtcggaggc ttcaactggc cggaacggct
      721 gcctggtgga tgaggcgcgc gagcaattcg acgccatcaa cgacacgcgg gccggggcgg
      781 ttgaagtagc tggagccgtc gaccgggtaa acgcggccct cgcgaacggc gcgcagtgta
      841 tgccaagcgg gaatgtcgtt tagctcgcgc atggcaacgc aggtggcggg caggtcgtag
      901 ccacaaggca tgacgatgat gacatccgaa tcggcaacgg cgatctgctc ccaggtgacg
      961 taggtcgagg gttggccctc cggcccgagc acgtcgacgc caccagccag ccgcaccatc
     1021 tccggcaccc agtgaccgcc aaccatcggc ggcgagagcc actccaggca gagcacagac
     1081 ggggctggcc ggtcgcccgt gcgttcggca accgccgcca gccgtgagcg caacgcggcg
     1141 acaacctctt cgccccgctc ccgcacgccg gccagccggg ccacctcgcc gatcgacgcc
     1201 agtatctcct cgatcgtgtg cggctcgaag gcggccacct ccggcacctt cggcagcgcc
     1261 tgcgccgccg ccagcacatc ggtatacgag acggcgcaga cctcgcacag ttcctgggtc
     1321 aggatcaggt cgggcgccag ctcggtcagc aggccggtgt cgaggtggta gatcgactgc
     1381 ccgctgagca cccgctcgct gatcatccgg tcgatgtcgg cgctgctgtg cggcccctcc
     1441 ggcaggacgt tggccgtcag cttcggcttc gcgagcgcac cgaccgggaa gtcgcactca
     1501 tgcgtcaccc cgacgagggc atcgcccagc cccagcgcgt aacagatctc ggtcgtgctc
     1561 ggcagcagcg aaacgatctt catcgtggcc tccgtggtgc gtgatgcgtc ccaggattga
     1621 actgctaccc aggatcgtac acctcacccc tgtcatcctg aaccgcagtg aaggatcgct
     1681 caaccgtaac agagggtgtg cgccggtcgg aggcacgcgt acggccaacc cgaaccgagt
     1741 tcgtcattca cgccaggcga tccttcactg cggttcagga tgacaggggg aatggtgctg
     1801 gtcctcgtca gtgtcgtacg tctcgaacac atcacatatc acgcgtcgcg ctgctagact
     1861 gcgcgcatcg tgcaatccgg atagcaggga acgagggacg tggatgagcg acgagcagcg
     1921 gccggcggag gatgcgccga acccggaact ggtcgaggcg attgtggcgc aggtgcgcgg
     1981 gttgatccag gacgagcgtg acgaggcggc gctggacgcg gtgcgccgca ccgccgagcg
     2041 gcacctgaag atggccgcca agctgcgcgc ctacccgttg cgcaatgccg acgagccgga
     2101 cttcgtcttc cggccatacc gggcggaggg ctagcagatg gccggcgaga tctacaacca
     2161 gacgatcgag gatctgggcc gcaagctgcg cgcccgggag acgacgccaa ccgagctgac
     2221 gcgcctctac ctggagcggc tcgattcggt cggacggcgc tacaacgcgg tcgtcacgat
     2281 cacggaggag cgggcgctgc gcgaggcggc ccaggccgag gccgagctgg cggccggcta
     2341 cgaccgcggc ccgctgcacg gcatccccta tggcgcgaaa gacctgctca gcgtgccggg
     2401 ctacccgacg acctggggcg cggtgccgta caaggatcag tccttcgacg aggagtcgca
     2461 ggtgacgacg aagctgcgcg acgccggcgc cgtcctggtc gccaagctgg ggatggtcga
     2521 gctggccggt gggatgggct acaaccagcc gaacgcttcg ctgaccggcc ccggcatcac
     2581 gccctggaac gcagacgagc atccgagctg gtcgggcggt tcctccagcg gttcgggttc
     2641 ggccgtcgcg tccggcgcgg tcggcttcgc gatcggctcg gagacgtggg gctcgatcac
     2701 gacaccggcc ggcttctgcg gcctctcggg cctgcggccg acctatggcc gtgtctcccg
     2761 gcgcggcgcg atggcgctct cgtggacgat ggacaagctc ggcccgatga cccgttcggc
     2821 ccacgactgc ggccttgtcc tcaacgcgat cgccggtcac gatcccggcg atccgagcag
     2881 cagccagcgg ccatacagct acccaccggc cgccgagcgg acgaccggtt tccgcttcgg
     2941 cctgctctct gacgcgacca cgaacgtcca ggacgcagtc cgcgacaact tcctcgcctc
     3001 gctgcgggtg ctggaggaga tcggcacggt tgagacagtc acgctgcccg acctgcccta
     3061 caacgaggtg gccgggatca tcatccaggc cgagctgtca tcggcgttcg aggagctgat
     3121 gaccagcggc gacgtgaccc agctgaccgc ccccgaggac cgcgtcaatg gcctggcgtc
     3181 gctggcgatg ccggccaacg tctacatccg cgcgctgcgc attcgccgcc acatctgcgt
     3241 cgcgatggac gagctgacct cgcgcttcga cgcgctgatc tcgccgacgc tgccgaccgt
     3301 cgccagcccg gtcgaccagt cgttcgaggc ctacttctcg cgcgccaacc gctccgcgct
     3361 cggcggggca gccaacgccg ccggcctgcc cggcatcgcc atccccaacg gctacggaga
     3421 acgggggctg ccgacgtcgc tccagttcac cggccgcgcc tgggatgaga acaccatcct
     3481 cgccgccgcc cgcgcctacc agtcccgcac ggactggcat acccgctggc cgtgctaacg
     3541 agttaagagt gctgcgtgct gagtcaaccg gtaggggcga ggcactcagc actcttaact
     3601 cagcactcgt ataaccccct ccccacccgc tgacttgtca tc
//
LOCUS       DASUGJ010000057         3431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1118486, whole genome shotgun sequence.
ACCESSION   DASUGJ010000057 DASUGJ010000000
VERSION     DASUGJ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118486"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1906)
                     /gene="ilvD"
                     /locus_tag="VFV93_01410"
     CDS             complement(209..1906)
                     /gene="ilvD"
                     /locus_tag="VFV93_01410"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5164025.1"
                     /translation="MVETKTEFDMRHKSRVMHDGRDRAAARAMLHAIGFSDDDLKKPV
                     VGVAHSWIGTMPCNFGLRRLAQQVMEGVRAAGGTPMEVNTVAISDGITMGTEGMKASL
                     VSRELIADSIELVARGHMFDAIIILAACDKTLPASAMALIRLDIPGFVLYGGTIYPGR
                     FQNRDVTVVDVFEGVGANAAGRMTDAELNELEHCAIPGAGACGGQFTANTMAMALEVL
                     GLSPTGMATVPQEDPTKQEIGFKAGELVMDQLKRGMRPSDILTRTSFDNMFAGACATG
                     GSTNIVLHSLAMAREAGIPLTIEDFNHISDRTPVIADLMPGGKYTAYDVYKAGGIQLI
                     TKRMIEGGVIDGSQLTPTGETLAEACADATETPGQQVITSVDQPLKPQGGLLVLKGNL
                     ASKGSVIKIAAADRYSHRGPARVFEREEDAMDAVTEGRIVAGDVIVIRYEGPRGGPGM
                     REMLGVTGALVGAGLGETVALLTDGRFSGGTRGFCIGHVAPEAAMGGPIAILRDGDMI
                     AIDVKERSLHVELTDDEISARFADWQPPKPQYTSGVFAKYAALVSTADEGAVTKPAL"
     gene            2122..2952
                     /locus_tag="VFV93_01415"
     CDS             2122..2952
                     /locus_tag="VFV93_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5164026.1"
                     /translation="MHATWNVLAKRSADPLAFLFAISAGGLVIYAVPVAIVLAREGVS
                     PRVVPFLLVSGVLEMAYTLFLAAAYRNGALSLTYPIARGTGVVLVPLLAIPLLDEQPT
                     GIALAGIATILVGFVTISVLGARDRVAEEVAHGRRGMMFALLTGFTIASYSLVDKAGV
                     QHANALIYVYGLIGMQSLLLLPYIVSRRRSAVAAAWATNRKAVIAGGVLHIGTYLIVL
                     AAMKVSGSKIGYIVPLRETSIVFATLLGVLLLNERIGNIRIAGSGLIAAGVLAIALGG
                     "
     gene            complement(3054..>3431)
                     /locus_tag="VFV93_01420"
     CDS             complement(3054..>3431)
                     /locus_tag="VFV93_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX5164027.1"
                     /translation="VSPRELALVPGHRVVESYFDDAILPRPPDGVLLVAPCSFNTLNK
                     LALGAADNLALSITAEAIGRGTPVIVAVSVNDPLWAHPSSQRSAADLRRWGVTVIDPI
                     PDQRGYLTMAPVETIVEHVVQAI"
BASE COUNT          547 a         1166 c         1126 g          592 t
ORIGIN      
        1 aggtcgccgc cggcgtggca cacgctccgg aaccggccac cgaactcgaa ctggagtacg
       61 agccggccgc tgactgaccc gcgaccatcg tctatccagc gaaacgcgtc acactctctg
      121 tgtggcgcgt ttcgtcatgt gctccgtcgt cctgagccgc agcggagagg tccttcgctg
      181 cggctcagga cgacaattgg gcgataacct acagcgcggg cttcgtcacc gcgccttcgt
      241 ctgcagtcga gaccagcgcg gcgtacttgg cgaagacgcc ggaggtgtac tgcggtttcg
      301 gcggctgcca gtcggcgaaa cgggccgaga tctcgtcgtc ggtcagttcg acgtgcagcg
      361 aacgctcctt gacatcaatc gcgatcatgt cgccgtcacg caggatcgcg atcgggccgc
      421 ccatcgccgc ttccggggcg acgtggccga tgcagaagcc gcgcgtgcca ccggagaagc
      481 gaccgtcggt cagcagcgca actgtctcgc ccaaaccagc gccgaccagc gctccggtga
      541 cgcccagcat ctcacgcatg ccgggaccgc cgcgcggacc ctcgtagcgg atgacgatca
      601 cgtcgccggc cacgatccgc ccctcggtaa cggcgtccat cgcgtcctct tcgcgctcga
      661 agacgcgcgc cggtccgcga tgactgtagc ggtcggctgc tgcgatcttg atgacgctgc
      721 ccttcgaggc caggttgccc ttgaggacca gcagcccgcc ctgcggcttg agcggctgat
      781 cgacgctcgt gatgacctgc tggccgggcg tctcggtcgc gtcggcgcag gcctcggcca
      841 gcgtctcgcc ggtcggcgtc agttggctgc cgtcgatgac gccgccttcg atcatccgct
      901 tggtaataag ctggatgcca ccggctttgt agacatcgta ggcggtgtac ttgccgcccg
      961 gcatcaggtc ggcaatgacc ggcgtccggt cggagatatg gttgaagtcc tcgatcgtca
     1021 gcggaatacc ggcctcgcgc gccatcgcca gcgagtgcag gacgatgttg gttgatccgc
     1081 cagtcgcgca ggcgccggcg aacatgttgt cgaagctggt ccgcgtcagg atgtcgctcg
     1141 ggcgcatccc gcgcttgagc tggtccatca ccagctcgcc ggccttgaaa ccaatctcct
     1201 gcttggtcgg gtcctcctgc gggacggtcg ccatcccagt tggcgagagg ccaagcacct
     1261 ccagcgccat cgccatcgtg ttggccgtga actggccgcc acaagcgccc gcaccgggaa
     1321 tcgcgcagtg ctcaagctcg ttcagctcgg cgtccgtcat ccgaccggca gcgttggcac
     1381 cgacaccctc gaagacatcg acaaccgtca catcccggtt ctggaagcgg ccggggtaga
     1441 tcgtgccgcc gtagaggacg aagccgggga tatcgagccg gatcagcgcc attgccgagg
     1501 ccggaagtgt cttgtcgcag gcagccagga tgatgatggc gtcgaacatg tggccgcgcg
     1561 cgaccagctc gatcgagtcg gcgatcagct cgcgggagac cagcgaggcc ttcatcccct
     1621 cggtccccat cgtgatgccg tcggagatgg cgaccgtgtt gacctccatc ggcgtcccgc
     1681 cggccgcccg gacaccctcc atcacctgct gcgccagccg ccgcaggccg aagttacagg
     1741 gcattgtccc gatccacgag tgggcgacgc cgacaaccgg cttcttgagg tcatcgtccg
     1801 agaagccgat cgcgtgcagc atcgcgcggg ctgccgcccg gtcgcgcccg tcgtgcatga
     1861 cgcggctctt gtgccgcatg tcgaattccg tcttcgtctc caccatcacc gtctccatca
     1921 accaattcac cggcgtgcat cggtagcagc acgccgccag cgagaattgc gtacccctcg
     1981 accgagcaat catcccgtgc cacgccacat tcggcaagtg ccatcccgcc ggcgccaaac
     2041 cgattgtcga ttgtcgccac acgccgtacc atgccgcgcg tgggagcagt agcggttggg
     2101 cttgtcctga tatcggcggt gatgcacgcc acctggaacg tgctcgcgaa gcgcagcgcg
     2161 gacccgctgg cgtttctgtt tgccatcagc gccggtggac tcgtcatcta cgccgtgccg
     2221 gtcgcaatcg tcctcgccag ggagggcgtc tccccccgcg tcgtcccatt cctgctggtc
     2281 agcggcgtgc tggagatggc ctacacgctg tttctggcgg cggcgtaccg caacggcgcg
     2341 ttgtcgctga cctacccgat cgcacgcgga accggcgtcg tcctcgtccc gctgctggcg
     2401 attccgctgc tggatgagca gccgacgggc atcgcgctgg ccggtatcgc gacgatcctg
     2461 gtcggcttcg tgacgatcag cgtcctcggc gcgcgggaca gggtcgccga ggaggtcgcc
     2521 cacggccggc gcgggatgat gtttgcgctg ctaaccggct tcacgatcgc gtcctactcg
     2581 ctggtcgata aggccggcgt ccagcacgcc aacgcgctga tctacgtcta cgggttgatc
     2641 gggatgcagt cgctgttgct gctgccctac atcgtcagcc gtcgcagatc ggccgtcgcc
     2701 gccgcctggg caacgaaccg gaaggccgtc atcgccggcg gcgtcctcca catcggcacc
     2761 tacctgatcg tcctggccgc aatgaaagtc tccggcagca agatcggcta tatcgtcccg
     2821 ctacgcgaga cgagcatcgt cttcgcgacg ctgctcggcg tcctgctgct caacgagcgc
     2881 atcggcaaca tccgcatcgc cggctccggc ctgatcgcag ccggcgtgct cgcgattgcg
     2941 ctgggtgggt agtctcggga cgctggacgc ggcgaggtga caacgcacac gaaccagagg
     3001 accggcgctg gcgagtaccg aatcccgatc gccggttccc gagacgagac tcgctagatc
     3061 gcctgcacca cgtgctcgac gatcgtctcg accggggcca tcgtcaggta gccgcgctgg
     3121 tccgggatcg ggtcgatgac ggtgacgccc cagcggcgca ggtcggcggc ggatcgctgg
     3181 gaggaggggt gcgcccagag cggatcgttg acggagacgg cgacgatgac cggcgtgccg
     3241 cgtccgatcg cttcggcggt gatcgagagt gcgagattgt cagctgcgcc caaggcaagc
     3301 ttgttgaggg tattgaaaga gcacggcgcg accagcagga cgccgtcggg tgggcggggc
     3361 aggatcgcgt cgtcgaagta gctctcgacg acgcgatggc ccggaacgag cgccagttcg
     3421 cgagggctga c
//
LOCUS       DASUGJ010000058         4467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_285640, whole genome shotgun sequence.
ACCESSION   DASUGJ010000058 DASUGJ010000000
VERSION     DASUGJ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285640"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFV93_01425"
     CDS             <1..541
                     /locus_tag="VFV93_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164028.1"
                     /translation="VLFISTHQDRLYPEQFGHLDQNGSGEGEGRTVNIPLPAGSGDAA
                     YLAAVEEIIMPVARQFKPELILVSAGQDASSADPLGRMAVTAEGYRRLTAAMIELATE
                     LSDGRLAVVQEGGYSETYAPYCTLAILEALTEYRSNLEEPVNQERADRWPQSQSVGLD
                     ARAALDAVKQQYAGYWDFS"
     gene            complement(645..1343)
                     /gene="rsmG"
                     /locus_tag="VFV93_01430"
     CDS             complement(645..1343)
                     /gene="rsmG"
                     /locus_tag="VFV93_01430"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX5164029.1"
                     /translation="MRLLTEHAGRLGVQLDAEAVERFRHYRDLLLAANERFNLTRITD
                     PNEVELRLFADSLSLLPHLPDDTTRLLDIGSGGGVPGMALAIAHPELPVTLLDATAKK
                     VRFLAETAAALGLEHVTAIQGRAEELARDPHHRELYSAVTARAVARLATLVELALPFL
                     ALGGRAILPKGSAAAEELAEARYAIGLLGGRARPPVTTPLEGTTLVIIDKRKPTPATY
                     PRNTGTPNKSPLRG"
     gene            complement(1353..3227)
                     /gene="dxs"
                     /locus_tag="VFV93_01435"
     CDS             complement(1353..3227)
                     /gene="dxs"
                     /locus_tag="VFV93_01435"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX5164030.1"
                     /translation="MYLERVSGPADLKTMTVPELEGLAREIRDEIYRVTSINGGHFAS
                     NLGSTELTLALHYVFDSPTDKIVWDVGHQGYPHKLVTGRRERFETIRKEGGLSGFLQR
                     EESEHDAFGAGHASTSVSAALGMAMANHLSGTKGRAIAVIGDGALTGGMAYEALNNAG
                     SLGIPFIVVLNDNEMSIAPNVGAVSKYLSRVRTDERYNRAKADFERMLQRLPQGELFV
                     ELGKRMKDSFKEFVYHTMIWEELGFTYVGPVDGHNVGDLIQALRQARHLDGPVFVHCV
                     TAKGKGFDFSEADPFKHHAARVASTGPSAPQPPKYQDVFGETLTELATENERIVAITA
                     AMPDGTGLLPFAKAHPERFFDVGIAEQHAVTFAAGMAAQGMRPVAAIYSTFLQRAYDQ
                     VIHDVCIQKLPVVFAMDRAGLVGDDGRTHHGVFDFAYLRCLPNMVVMSPKDEAELRNM
                     LLTAVEHTSGPIAVRYPRGNGVGVSTSIPPHTLPIGRGELLREGTDVTIVSIGTMAMT
                     AEHTADLLAQEGISAAIINARFVKPLDEALILDWAKRTGAVVTLEEGAAAGGFGAAVL
                     ELLAREDMQIPVKVLGIPDRFFDHASQTSLRKQAGLSIEHVVEAARAVVRQLAPVVTS
                     "
     gene            3617..>4467
                     /locus_tag="VFV93_01440"
     CDS             3617..>4467
                     /locus_tag="VFV93_01440"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5164031.1"
                     /translation="MSRLDVWRNLLIRSRMLVAAALVLGAALSGVPAVPAPTSAQSSL
                     SLVPVTGNLDAPVAMTNAGDGTNRLFVVERSGRIRVIVDGQLQETPFLDLTDRVHTDD
                     SEQGLLALAFHPDYENNGELFVLYTAEDWANTVERFTVSTNDPNRADPASGLILLATP
                     DREPNHNGGSLVFGPDGMLYITTGDEGGGGDPYRNAQNLSSLFGKILRVDVDGGDPYT
                     IPPDNPFVDTENARPEVWVYGLRNPWRISFDHETSDLWVADVGQATWEEVNYVPAGTG
                     AGRNFGW"
BASE COUNT          769 a         1462 c         1464 g          772 t
ORIGIN      
        1 tgtcctcttc atctcaaccc atcaggatcg cctttacccc gagcagttcg gccacctcga
       61 tcagaacggc tccggcgaag gcgaggggcg caccgtcaac atcccgctcc cagccggcag
      121 cggcgacgcg gcctacctgg ccgccgtcga ggagatcatc atgccggtcg cccgtcagtt
      181 caagccggaa ctgatcctcg tctcagccgg tcaggacgca tcctcagccg acccgctcgg
      241 ccggatggcg gtcacggccg aaggctaccg caggctgacg gcggcgatga tcgagctggc
      301 gaccgagctc agtgacggcc ggctggcggt cgtccaggag ggcggctact cggagactta
      361 cgccccttac tgcacgctgg cgatcctcga agcactgacc gaataccgct ccaacctcga
      421 agagccggtc aatcaggaac gggccgaccg gtggccgcag tcccaatcgg tcgggctgga
      481 tgctcgggca gcgctggatg cggtcaagca gcagtatgcg gggtattggg atttcagctg
      541 acgcttcgac ctccacaacc aatggtcaat ccggtacggg cgatattgcg atatcgcccg
      601 taccggattg cattgacgtt tggagcgaac aggaatgatt ggattcatcc ccgcagcgga
      661 ctcttgttcg gcgtgcccgt attccttgga tatgtcgcgg gggttggctt gcgcttatcg
      721 atgatgacca gcgtcgtgcc ttcgagcggg gttgtcacgg gcggacgcgc gcggccaccc
      781 agcaggccga tcgcgtagcg tgcttcggcc agttcctcgg cggcggcgct gccctttggc
      841 agaatcgcgc gtccgccgag cgcgaggaac ggcagggcca gctcgaccag cgttgccagc
      901 cgggcgacgg cccgggcggt aacggcgctg tacagttcgc ggtgatgcgg gtcgcgagcc
      961 agttcctcag cccggccctg gatcgccgtc acgtgctcca gccccagcgc cgcggccgtc
     1021 tcggccagga aacggacctt cttggcggtc gcgtcgagga gcgttactgg tagttcaggg
     1081 tgggcgattg ccagcgccat gcccggcacg ccaccgcccg aaccgatgtc gagcagacgc
     1141 gttgtatcgt ccggcaggtg tggcagcagc gagagtgagt cggcgaagag ccgcaactcg
     1201 acctcgttgg gatcggtgat acgggtgagg ttgaagcgct cgttggcagc cagcagcagg
     1261 tcgcgatagt gccggaaacg ttcgaccgcc tcggcgtcca gctggacgcc gaggcggccg
     1321 gcatgctccg tgaggagtcg catcgtctgt ggctagctgg tcacgacagg cgccagctgg
     1381 cgaacgacag cgcgcgcggc ctcgacgaca tgctcgatcg agaggcccgc ctgcttgcgc
     1441 agcgaggtct gcgaggcgtg gtcaaagaag cggtccggga tgccgagcac tttgaccggt
     1501 atctgcatgt cttcacgagc cagcaactcc aggacggcgg caccgaaccc accagccgca
     1561 gcgccttctt cgagcgtcac gaccgcgccg gtgcgcttgg cccagtcgag gatcagcgcc
     1621 tcgtccagcg gcttgacgaa gcgggcgttg atgatggctg ccgaaatgcc ctcctgcgcc
     1681 agcaggtcgg cggtgtgctc ggccgtcatc gccatcgtcc cgatcgagac gatcgtgacg
     1741 tcggtgcctt cgcgcagcag ctcgcctcgc ccgatcggca gcgtatgcgg cgggatcgag
     1801 gtcgacacgc caacgccgtt gccacgcggg taacgtaccg caatcggacc gctggtgtgc
     1861 tcgacggccg tcagcagcat gttgcgtagc tcggcctcgt ccttcggtga catcacgacc
     1921 atgttcggca ggcagcgcag gtaggcgaag tcgaagacgc cgtggtgggt ccggccatcg
     1981 tcgccgacca gcccggcccg gtccatcgcg aagacgaccg gcagcttctg gatgcagaca
     2041 tcgtggatga cctgatcgta ggcgcgctgg agaaaggtcg agtagatcgc cgcaaccggc
     2101 cgcatcccct gagccgccat cccggccgcg aacgtcacgg catgctgctc ggcgatgccg
     2161 acgtcgaaga agcgctccgg gtgggccttc gcaaagggca acagccccgt cccatccggc
     2221 attgcggcag tgatcgcgac gatccgttca ttttcggtgg cgagttcggt cagcgtctcg
     2281 ccaaagacat cctgatactt gggcggctgc ggcgccgacg ggccggtgga agccacgcgg
     2341 gcggcgtggt gcttgaatgg gtctgcctcg gagaaatcga agcccttgcc cttggctgtc
     2401 acgcaatgga cgaagacagg accgtcgaga tggcgtgcct gcctgagcgc ctgaatcaga
     2461 tcaccgacgt tgtggccgtc gaccgggccg acgtaggtga agcccagttc ctcccagatc
     2521 atcgtgtggt agacgaattc cttgaaggag tccttcatcc gcttgccaag ctcgacgaac
     2581 agttcgccct gtggcaagcg ctgcagcatc cgttcgaaat ctgctttggc gcggttgtag
     2641 cgctcgtcgg tccgcactcg cgagaggtat ttcgagacgg cgccgacgtt cggcgcgatg
     2701 ctcatctcgt tatcgttgag gacgacgatg aacggaatcc caaggctgcc ggcgttgttg
     2761 agcgcctcgt aggccatccc gcccgtcagc gcgccgtcgc cgatgacggc gatcgcccgg
     2821 cccttcgtgc cgctgaggtg gttggccatc gccatcccga gtgcggccga gacgctggtg
     2881 ctggcgtgac cggcgccaaa cgcgtcgtgc tcgctctctt cgcgctggag gaagccggag
     2941 agcccgcctt ccttgcggat cgtctcgaac cgctcgcgcc ggccggtcac cagcttgtgc
     3001 gggtagccct ggtggccgac atcccagacg atcttgtcgg ttggcgaatc gaagacgtag
     3061 tgcagggcaa gcgtcagttc agtcgagccg aggttcgagg cgaaatgacc gccgttgatg
     3121 ctggtgacgc ggtagatctc gtcccggatt tcccgcgcca agccctctag ttcaggcacg
     3181 gtcatcgtct tgaggtcggc gggacccgaa actcgctcaa gatacatgtg ctggttcctc
     3241 caggcgcggt gactcgccca atcccccatc agtcacgggc cgaccgcacc acccttaatg
     3301 tcgcgcacga ggcgtgccgc gtatcgcgat ggtaacaagc cgctgaaatg ggcgtcaaac
     3361 caaggcgtca gcgcacaact cactgccata caacctgctg tcgtcacaat aagccgaatc
     3421 tgaatggtcg cgttacgatg ccgtaacgtg gcgacagggc ggcacggatg cgccgccctg
     3481 tctgctcgcc aagcatcgag caattctacc accggccgcc gtcgctacgg tctgaactgc
     3541 ggcgtcagtc atgcgctgac gctggacaat gcatactggc cgtcggaatc ggacggtgga
     3601 ggacgagggg gagcgagtgt cacgtctcga cgtttggcgg aatctgctta tccggagccg
     3661 gatgctggtg gcggccgcgc tcgtcctcgg cgcggccctg agcggcgttc cggccgtgcc
     3721 agcgccaacc agcgcccaat cgtcgctgtc gctcgtcccg gtcaccggta acctggacgc
     3781 gccggtcgcg atgactaacg ctggcgacgg cacgaacagg ctgttcgtcg tcgagcggtc
     3841 cggccggatc cgcgtcatcg tcgatggcca gttgcaggag acgccgtttc tcgacctgac
     3901 cgaccgcgtc cacaccgacg acagcgaaca gggcctgctg gcactggcgt tccatcccga
     3961 ctacgagaac aacggcgagc tgttcgtcct ctacacggcc gaagattggg ccaacacggt
     4021 cgaacgcttc accgtctcga ccaacgaccc caaccgcgcc gacccggcca gcggcctgat
     4081 cctgctggcg actcccgacc gcgagccgaa ccacaatggc ggctcgctcg tcttcggccc
     4141 ggacggcatg ctctacatca cgaccggtga cgaaggcggc ggtggagatc cgtaccgcaa
     4201 cgcgcagaac ctgtcgtcgc tgttcggcaa gattctgcgg gtcgatgtcg atggcggcga
     4261 cccctacaca atcccgcccg acaatccgtt cgtggatacc gagaacgctc gccccgaagt
     4321 ctgggtctac ggcctccgca acccctggcg catcagcttc gaccacgaga ccagcgatct
     4381 ctgggtcgcg gatgtcggtc aggcgacctg ggaagaggtc aactacgtcc cggccggcac
     4441 cggcgcgggt cgcaacttcg gctggag
//
LOCUS       DASUGJ010000059         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_842004, whole genome shotgun sequence.
ACCESSION   DASUGJ010000059 DASUGJ010000000
VERSION     DASUGJ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842004"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1980
                     /locus_tag="VFV93_01445"
     CDS             <1..1980
                     /locus_tag="VFV93_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor GTPase family protein"
                     /protein_id="HEX5164032.1"
                     /translation="VSWMAIINIGILAHVDAGKTSLTERILFETGVIRTIGSVDKGTT
                     QTDTREIERARGITIWSAVVSFQLNQLKVNLIDTPGHGDFIAEVERSLRVLDGVVLVV
                     SAVEGVQSQTRRLARAIQAAELPLLIFVNKIDRLGARVEPLLEDIQRKLKLRIVTLNT
                     AVNTGDRDACVIACDRDSPAWQDPLLDLLAETSEDVIEEFERTGGDVGRPFLEAELRR
                     QVAAGSVVPVFFGSAMTGAGVGELLGGVEEWLPPACEATDEPATGTIFKIARRPSGEK
                     IVYARLFEGSLAVRQRVVARRRNQFGELVENEERITAIDQYADGVTTHAGEVRAGEIA
                     GLHGLRAARIGDQIGVDDGRHLDLAPAFPAPKLESIVRPVDPGQIARLREALEQLAEQ
                     DPLMSLRQRNEAGEISVRLYGEVQKEVVTDTLLREYGVEAWFGPSQIICVERPVGTGE
                     AFEIIGEGDNPFYGTVGFRVEAAEAGSGIRYVRELGSLPLAFYRAIEETVYETFQQGL
                     AGWEVTDCVITLTHAGFWSPVSTAGDFRKLTPLPLMDALRQAATTVCEPYEELEIDIP
                     EDTFGGVCGALIAARATIGNVYRDGTSQRVVCQIPTAELRAIEQQLPGLTRGEGGWST
                     TFAGYIPVYGEPPTRARIGPNPLNRVQYLGDVARL"
BASE COUNT          374 a          736 c          790 g          344 t
ORIGIN      
        1 gtgtcgtgga tggcgatcat caacatcggg attctggcgc atgtcgacgc cggtaagacg
       61 agcctcaccg aacgaatcct cttcgagacc ggcgtcattc gaacgatcgg cagtgtcgac
      121 aagggcacga cccagaccga cacgcgggag attgagcgcg ctcgcgggat cacgatctgg
      181 tccgcggtcg tctcgttcca gctgaaccag ctcaaggtca acctgatcga cacccccggc
      241 cacggtgact tcatcgccga ggtcgagcgc tcgttgcggg tgctggacgg cgtcgtcctg
      301 gtcgtctcgg ccgtcgaggg tgtccagtcg caaacccggc ggctggcgcg tgccatccag
      361 gcggcggaac tgccgctgct gatcttcgtc aacaagatcg accggctcgg agcgcgggtc
      421 gagccgctgc tggaggacat ccagcgcaag ctgaagttgc ggatcgtgac gctgaacacg
      481 gccgtcaaca cgggagaccg cgacgcctgc gtcatcgcct gcgaccgtga cagtcccgca
      541 tggcaggatc cgctgctcga tctgctggcc gagacaagcg aagacgtgat cgaggagttc
      601 gagcggacgg gcggcgatgt tggccgtccg ttcctggaag ccgaactgcg gcggcaggtc
      661 gcggccggca gcgtcgtgcc ggtcttcttc ggctcggcga tgaccggcgc cggcgtgggg
      721 gagctgctgg gcggcgtcga ggagtggctg ccgccggcct gcgaagcgac cgatgagcca
      781 gcgaccggca cgatcttcaa gatcgcgcgg cggccgtctg gggagaagat cgtctacgca
      841 cgactgttcg agggcagtct ggctgtgcgc caacgggttg tcgcgcggcg caggaaccag
      901 ttcggggagc tggtcgagaa cgaggaacgt atcaccgcca tcgaccagta tgcagacggc
      961 gtgaccaccc acgccggcga ggttcgcgcc ggtgagatcg ccgggctgca cggcctgcgg
     1021 gcggcccgga tcggcgacca gatcggcgtc gatgacgggc gccatctcga tctcgcgccg
     1081 gccttcccag ccccgaagct ggagagcatc gtccgacccg tcgatccggg ccagatcgcc
     1141 cgcctgcgcg aggcgctgga gcaactggcc gagcaggacc cgctgatgtc gctgcggcag
     1201 cgcaacgagg ccggcgagat ctcggtccgg ctctacggcg aggttcagaa ggaggtcgtg
     1261 accgacacac tgctgcgcga gtacggcgtc gaagcatggt tcggccccag ccagatcatc
     1321 tgcgtcgagc ggccggtcgg gacgggcgaa gcgttcgaga tcatcggcga gggcgacaac
     1381 ccgttctacg gcacggtcgg cttccgggtc gaagcggccg aagccggcag tggcatccgc
     1441 tacgtccgcg agcttggctc gctgccgctg gcgttctacc gcgcgatcga ggagacggtc
     1501 tacgagacgt tccagcaggg gttggccggc tgggaagtca ccgactgcgt catcacgctg
     1561 actcacgctg gcttctggtc gccagtcagc acggccggcg acttccgcaa gctgacgccg
     1621 ctgccgctga tggacgcgct ccgccaggcc gcgacaaccg tctgcgagcc gtacgaggag
     1681 ctggagatcg acatccccga ggacacgttc ggcggggtct gcggcgcgct gatcgccgcc
     1741 cgcgcgacga tcggcaacgt ctaccgcgac ggcacgtcgc aacgggtcgt ctgccagatc
     1801 ccgaccgccg aactgcgcgc gatcgagcag cagctaccgg gcctcacccg cggcgagggc
     1861 ggctggtcaa caaccttcgc cggctacatc cccgtctacg gcgaaccgcc aacacgggcg
     1921 cggattggac cgaacccgct caatcgggtg cagtatctcg gcgatgtggc gcggctgtaa
     1981 tgccgcaatc ccaatcccca atccggtacg ggcgatatcg caatatcgcc cctggacttt
     2041 ggcgatgtca gcccgtcggg tcgtcggtcg cgttgtgtgg gcggctcagc cgcgttgggc
     2101 ggccgtgccg gcggtggtca gtgacccgag gtgggcggga atatcccacc agtcgtgcgg
     2161 caaccgcgtg ccagcgggct gaaagtcccc ggcggcccag acttcgtcgc gcatggcctg
     2221 ccacaggtga ccgaacgacc agcg
//
LOCUS       DASUGJ010000060         4476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_285947, whole genome shotgun sequence.
ACCESSION   DASUGJ010000060 DASUGJ010000000
VERSION     DASUGJ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285947"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFV93_01450"
     CDS             complement(<1..270)
                     /locus_tag="VFV93_01450"
                     /inference="COORDINATES: protein motif:HMM:NF036497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5164033.1"
                     /translation="MGVERVLDISQPTRRRRTALAPKRPGEVQHVAPGSLAEEIGIEP
                     GDRVVKVNNQPLRDILDFQFLAAEEEVILEIERAGELHICEVERDL"
     gene            complement(297..1025)
                     /locus_tag="VFV93_01455"
     CDS             complement(297..1025)
                     /locus_tag="VFV93_01455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX5164034.1"
                     /translation="MAVPRFYVSDALEQGATGVLDSNQSRQARSVLRLTTSASLILFN
                     DSGVEADARIVSLANGGVTYEIIAIGRPDREPNLRLTVGLALLKGDRFEVAVQKLTEL
                     GVARIAPMLTERSVVSFDDARGWQKRSERYARIAREAAEQSERVTLPVITAPVTLAEF
                     LRQQPVIVLLERAESAPIATHAPGPEAAIAVGPEGGWSDAEQALIVREAAGTASLGRL
                     ILRAETAAIVAAGTVIQRAWEAQP"
     gene            complement(1030..1953)
                     /gene="prmA"
                     /locus_tag="VFV93_01460"
     CDS             complement(1030..1953)
                     /gene="prmA"
                     /locus_tag="VFV93_01460"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX5164035.1"
                     /translation="MAEEAHGWVEVSVAANAESVEAVSELLARYGYNEGVAIEEPYQQ
                     DEDGDNLEIDPTRPVIVRTWLAVDASLNEQRTAIEQGIWHLSRIGDVGSPEYTERAEE
                     DWANAWKEHFPILKIGRHFVVRPSWRDYDAEPGDLLIHLDPGMAFGTGLHPSTEMCML
                     LMEDLDLTGLDVLDVGAGSGILSIGAIRQGASKAVAVEIDSVAVRSLVENVALNEMQA
                     QIEVIGGDIKQALGAERTFPVVFANLIARILADNAEAIASHVASPGIIIASGIIEERE
                     QLVLDAFSARGFRVTDRRQARDWVALRLERG"
     gene            complement(1946..2944)
                     /locus_tag="VFV93_01465"
     CDS             complement(1946..2944)
                     /locus_tag="VFV93_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5164036.1"
                     /translation="MNVIIPVGGLGTRLRPFTWSRPKPLVSVAGKPLLSHVLDSLQAL
                     TLDRVVFVTGFLGEQIEDFVRKNFDFDAVFVRQDEPLGQSHAILQARGHITGPTMIVF
                     PDMIFEADLPRLETLSSDGAIFVKQVDDPRRFGVVELDGDRVTAFTEKPETPVSNLAI
                     MGIYYVKEIRKLFSAIDRQMGQRLQTQGEYFLADALQMLIDEGDEFTTIPATVWEDCG
                     TIKALLGANRFLLSQMDTPADVPGSVIIPPVYIAPTARVVSSVIGPHASIGDGVAVED
                     AIVRDSIVDSGATIESTVLVSSIVGRNAVVRGEPMRVNVGDSSDVDLRSMQENGRG"
     gene            complement(3181..3254)
                     /locus_tag="VFV93_01470"
     tRNA            complement(3181..3254)
                     /locus_tag="VFV93_01470"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3218..3220),aa:Glu,seq:ttc)
     gene            complement(3305..>4476)
                     /locus_tag="VFV93_01475"
     CDS             complement(3305..>4476)
                     /locus_tag="VFV93_01475"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX5164037.1"
                     /translation="VEFRWQDATGIVRDSTLYMPPAPRVGRGDRVEVVGETLDLNGKR
                     VFADGVRIVKQTGRLETERRRIRTYLTESIQRHVPGTPGSLTLGLLIGDDTALTNAER
                     DDLRHAGLSHLTAVSGWNVTLVTGTVGLLFLRLGLRGWRWTVLQLIALAGYVWIVGLD
                     PPVTRAAIMVVAGLVAIRLGRPAHSLTVLVLSASLMVAVSPASLASLAFQLSVLATLG
                     LIVASRLTAGFEGWRAIALTPVIATAATGVITAPLLASEFGTLSLATVPANILAAPLV
                     PAATIAGIAVIALGPVTPLATAAGWVAWSLCALLLGLARTLASVPHGYHEFAPLSDAQ
                     QAGLYAALLVIVAAVLPEGRLLLRAAVNWARQEPTGAALSASAACVALLAATFTV"
BASE COUNT          788 a         1470 c         1463 g          755 t
ORIGIN      
        1 aaggtcgcgt tcgacctcgc agatatgcag ctcaccggcg cgctcgatct cgaggatcac
       61 ttcctcctcg gccgccagga actggaagtc gaggatgtcg cgcaagggct ggttgttgac
      121 cttgacgacc cggtcgcccg gctcgatgcc gatctcctcg gccagcgaac ccggcgcgac
      181 gtgctgcact tcgcccggtc gcttgggcgc cagcgccgtt cgacgcctgc gggttggttg
      241 ggaaatgtcc agaactcgtt cgacgcccac gacggccgac tgcctccctg cccctgctac
      301 ggttgtgctt cccatgcgcg ctggatcacc gtgccggcgg cgacgatcgc cgccgtctcc
      361 gcccgcagga tcaatcgtcc cagcgaggcg gttccggccg cctcccgcac gatcagcgcc
      421 tgctccgcgt ccgaccagcc gccctccggc ccgaccgcga tggccgcttc cggacccggc
      481 gcgtgcgtcg cgatcggtgc cgactcggcc cgttcgagca gcacgataac cggctgttgg
      541 cgcaggaact cggcgagggt cactggagcc gtgatcaccg gcagcgtcac gcgctcggac
      601 tgttccgcgg cctcgcgggc gatacgcgcg tagcgctcgg agcgcttctg ccagccgcgc
      661 gcatcgtcga aggataccac gctacgttct gtcaacatcg gcgcgattcg cgccacacca
      721 agctccgtca gcttctggac ggcgacctcg aagcggtccc ctttcagcag cgccagcccg
      781 accgtcagcc gcaggttcgg ttcacgatcc ggccgaccga tggcgatgat ttcgtaggtg
      841 acgccgccgt ttgcgaggga cacgattcga gcgtccgctt caacgccgct atcattgaag
      901 aggatcagcg aagcgctggt tgtgagtcgg agaacggatc gtgcctggcg cgactggtta
      961 gagtcgagga cgcccgtggc accttgttcc agcgcatcgg agacgtagaa gcgtggaacg
     1021 gccatcgggt caaccgcgtt cgagccgcag cgcgacccag tcccgtgcct gacggcggtc
     1081 cgtcacacgg aacccgcgcg cgctgaatgc atccagcacg agctgttcgc gttcctcgat
     1141 gatgccactc gcgatgatga tgcccggcga cgcgacgtga ctcgcgattg cttcggcgtt
     1201 gtcggccaga attcgcgcga tcagattggc gaagacgacc gggaaggtgc gttcagcgcc
     1261 gagggcttgc ttgatgtcgc cgccgatgac ttcgatctgc gcctgcattt cgttgagggc
     1321 gacgttctcc accagcgagc gaaccgcgac gctgtcgatt tcgacggcga cggccttcga
     1381 tgcgccctgc cgtatcgcgc cgatcgagag gatgcctgaa ccggcgccga cgtcgaggac
     1441 gtccagcccg gtcaggtcca gatcctccat cagcagcatg cacatctcgg ttgagggatg
     1501 caggccggtc ccgaacgcca tcccgggatc gaggtggatc agcagatcac caggctcggc
     1561 gtcgtagtcg cgccagcttg ggcgaacgac gaagtggcgg ccgatcttga gaatcgggaa
     1621 atgctctttc caggcgttgg cccagtcctc ctcggcccgt tcggtgtatt ccggggagcc
     1681 gacatcgccg atccggctca ggtgccagat gccctgctcg atggccgtgc gctgctcgtt
     1741 caggctggcg tcgaccgcca accaggtacg gacgatgacc gggcgggtcg ggtcaatctc
     1801 gaggttgtcg ccgtcttcgt cctgctggta gggctcctcg atcgcgacgc cctcgttgta
     1861 gccgtagcgc gccagcagtt cgctgacggc ttcgaccgac tcggcgttgg cggcgacgct
     1921 gacctcgacc cagccgtgcg cttcctcagc cacggccgtt ctcctgcatc gagcgcagat
     1981 cgacgtccga cgagtcgccg acgttgacac gcatcggctc gccccggaca acagcgttgc
     2041 ggccgacgat cgatgagact agcacggttg actcgatcgt cgcgccggag tcgacgatcg
     2101 agtcgcgcac gatcgcgtcc tcgacagcca cgccgtcgcc gatgctggcg tgcgggccga
     2161 tgaccgaact gacgaccctg gcggttgggg cgatatagac cggcggaatg atgacggaac
     2221 cgggaacgtc ggcgggcgtg tccatctgcg acagcaggaa gcggttggct cccagcagcg
     2281 ccttgatcgt tccgcaatct tcccagaccg ttgccggaat cgttgtgaac tcgtcgcctt
     2341 catcgatcag catctgcaag gcgtccgcca gaaaatactc gccctgtgtc tgcaagcgct
     2401 gccccatctg ccggtcgatc gcggagaaca gcttgcggat ctccttgacg tagtagatgc
     2461 ccatgatggc caggttggag accggcgtct cgggcttctc ggtgaaggcg gtgacgcggt
     2521 cgccgtccag ttctacgaca ccgaagcggc gcggatcgtc gacctgcttg acgaagatgg
     2581 cgccgtctga actcaacgtt tcgaggcgcg gcagatccgc ttcgaagatc atgtcgggga
     2641 agacgatcat cgtcgggccg gtgatgtggc cgcgtgcctg caggatggcg tgcgactggc
     2701 cgagtggttc atcctggcgg acgaagacgg cgtcgaagtc gaagttcttg cgaacgaaat
     2761 cctcgatctg ctcgccaagg aagccggtca cgaagacgac gcgatccagt gtcagtgcct
     2821 gcaaggagtc gaggacatgg ctgaggaggg gtttgccggc gacgctcacc agtggcttgg
     2881 gccggctcca ggtgaacggt cggaggcgcg ttcccagtcc accgacggga atgatgacgt
     2941 tcattcggcg atccacctct gcttacgttt cgacacagcc ccgtctccgc gcggcaatca
     3001 catcaatgtg acggcctgcg cactcaagag tgtacccgaa cgatgcggcg ccgaagtcac
     3061 gaatccagcg agaatcgatg aagtgcatga cagaaaggtc cggcccaacc gtcgcgggca
     3121 agcgcgacac atttcacgcg atgtcgcgcg gcaagtctgg gcgttactga gagcgggagg
     3181 tacccccaag gggattcgaa cccctgatct cctccttgaa agggaggtgt cctaggcctc
     3241 tagacgatgg gggcatcgtc gccttgcggc gtggctgtag gatacgcagc cgttcggaca
     3301 aatgtcaaac cgtgaacgtt gccgccagga gagcgacgca ggccgcactc gccgagagcg
     3361 ccgcgccggt tggttcttgt cgagcccagt tcacggccgc ccgaagcagc agcctgccct
     3421 ccggcaagac cgccgcgacg atcaccagca gtgctgcata cagcccggcc tgctgggcat
     3481 ccgagagcgg ggcgaactca tgatatccgt gcggcaccga cgcgagcgtc cgggccagcc
     3541 cgagcagcag cgcgcagagt gaccacgcga cccaacctgc ggccgttgcc agcggggtaa
     3601 cgggaccgag cgcgatgacg gcgatccccg cgattgtcgc ggccggtacg agcggggcgg
     3661 caaggatgtt agctggaacg gtcgccagcg acagcgttcc gaactcgctt gccagcagag
     3721 gcgccgtgat gacacccgtc gctgcagttg cgatgaccgg cgtgagcgca atcgctcgcc
     3781 aaccttcgaa tcctgccgtc aatctgctgg cgacgatcag tcccagcgtc gccagcacac
     3841 tcagctggaa cgccagcgac gccagcgacg ccggcgagac ggcgaccatc aggctggccg
     3901 acaggaccag caccgtcaac gagtgggccg gccgtccgag ccgaatcgcg acgagtccag
     3961 caacgaccat gatggccgcg cgtgtcacgg gcggatcgag gcccacgatc cagacgtaac
     4021 cggcaagagc gatcaactgg aggacggtcc agcgccagcc gcgcaggccg agccgcagga
     4081 acaacagccc gacagtgccg gtcaccagcg tcacattcca gccgctgacg gccgtcaggt
     4141 gcgacagacc ggcgtggcga aggtcgtccc gttcggcgtt cgtcagcgcg gtgtcgtcgc
     4201 caatcaacag cccgagcgtc agcgagccgg gcgtgccggg aacgtggcgc tgaatcgatt
     4261 cggtcaggta ggtacggatg cgacggcgct ctgtttcgag ccggccagtc tgcttgacga
     4321 tccgtacccc gtcggcaaac actcgcttgc cgttgagatc cagcgtctcg ccgacgactt
     4381 cgacgcggtc gccacgaccg actctcggtg ccggcggcat atagagcgtg ctgtcgcgga
     4441 cgataccagt tgcgtcctgc catctgaact caacgg
//
LOCUS       DASUGJ010000061         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1814779, whole genome shotgun sequence.
ACCESSION   DASUGJ010000061 DASUGJ010000000
VERSION     DASUGJ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814779"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFV93_01480"
     CDS             complement(<1..1002)
                     /locus_tag="VFV93_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164038.1"
                     /translation="MLTFTRAGQSTLYVEDPIDPASFGILGFLLPENTPHLPTQLTIE
                     ESWYWKNNTLGAGWYIFIAPGVAFPNPKSESQLLSQFVTEMRKSLPSVPAQQSSGFAW
                     VVGDPQQPTAMSVVRLDMVGTGNRNSGNNPSTSRYDQTFTFKQFVITVAEKTEVQIKS
                     DFSGFEILSTLNEPTVHMHIPVAPYVYRPATTVTVPFSGEGRGALQFTLEPLLPTQFG
                     YGALDVSLFYFLRPNGTNGSTAPQLLRYPIIDAPHAEGTFEFQANLYHVDQRSSFFTV
                     KDLEDGTPKRIASYFRTTAGFPVHLRSSDNARLYLRARPNLNPAISDLEQIYYLAPSG
                     "
     gene            1563..2279
                     /locus_tag="VFV93_01485"
     CDS             1563..2279
                     /locus_tag="VFV93_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX5164039.1"
                     /translation="MEPPKPRQQRRRPAQPRSTTREPRPLAAGTITKVTPTQRDPERI
                     SVFIDGTFVFALPDIIVATRGLKRGVELTLEQVRELAGIAEGEKATAAALTFVSYRPR
                     SEREVRDRLRRKAYEPAAIDYAIEKMRGWRYLDDTKFAEFWVESRAEHAPRGKRALQS
                     ELRAKGVDREVVERVLDGTDLDERSGALEIARKRLRSLSSYDEETQRRRLAAFLARRG
                     YGWDVVKPVMAELFGGDGDG"
     gene            2430..>3015
                     /locus_tag="VFV93_01490"
     CDS             2430..>3015
                     /locus_tag="VFV93_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX5164040.1"
                     /translation="MSGRTGGTATEARTTTSGIEIAPVYRADGREPEPDPGQYPYTRG
                     VYPDMYRGRLWTMRQYAGFSSAEETNQRFKLLLARGQTGLSCAFDLPTQLGLDADDPR
                     AEAEVGIVGVSISTLDDMRRLLDGITLDDVTTSMTINAPASILMLMYQIVGEEQGANL
                     ARLGGTIQNDILKEYAARGTYIFPPRQSMRLVTDT"
BASE COUNT          592 a          967 c          929 g          527 t
ORIGIN      
        1 gccgctggga gccaggtagt atatctgctc caagtcgctg atcgccgggt tcaggttcgg
       61 ccgtgcccgc aggtagagcc gagcattgtc gctagagcgg agatgcaccg ggaacccggc
      121 cgtcgtgcgg aagtacgagg caattcgctt cggcgtcccg tcctccaagt ccttgaccgt
      181 gaagaacgat gacctctgat cgacatggta gaggttcgcc tggaactcga acgttccttc
      241 ggcgtgcggc gcatcgataa tcggataacg cagcagctgc ggtgctgtcg agccattcgt
      301 cccgttcggg cggaggaagt aaaacagcga cacatcgagc gcaccatagc cgaactgtgt
      361 cggcagcaat ggctcgagcg tgaattgcag cgcgccgcga ccttctcctg aaaacggcac
      421 cgtgaccgtc gtcgccggcc gatagacata cggcgcgact ggtatgtgca tatggaccgt
      481 cggctcgttg agcgtcgaca aaatctcgaa gccggagaaa tcggatttga tctggacttc
      541 agttttttcg gcgaccgtga tcacaaactg cttaaatgtg aaggtctggt cgtagcggct
      601 cgttgatgga ttgttgcccg aattccgatt gccggttccg accatgtcca accgaacgac
      661 cgacattgcc gttggctgct gcggatcgcc aacgacccat gcaaagccag agctctgctg
      721 cgcgggaacg ctcggaagcg acttgcgcat ctctgtcacg aactggctta ggagttgaga
      781 ttcgctcttt ggattgggaa aagcgacccc tggagcaatg aagatgtacc agccggcgcc
      841 caaggtgttg ttcttccagt accaactctc ttcgatcgtc aattgtgtgg ggaggtgagg
      901 agtgttctcg ggtagtagaa agccgaggat tccgaaactt gcaggatcga ttggatcctc
      961 gacgtataaa gtcgactggc ctgcacgggt aaacgtcagc acgatagcct ccgtgaggcg
     1021 aaaagacgag tgttgtgccg gcaagaatgg cgtaccagca gatagcccgc aggctagcgc
     1081 ctgaggccaa agaaccccgt caaatcgcgt gtattcctca aaccccgcgg tgcaggctgt
     1141 cacagctgtg caggtagcga ccgaagcaca tccccccgga gtgcttagcg tgccagaacc
     1201 tgtttcacac aggctaaatg accggcgcag taacgcgtca acgcgagcaa ctgcgcatct
     1261 ggaggtcgcc gaaccagtgt gagcctggca ggatactacc tgtcggactt tcgcgacagg
     1321 aggacgagaa tttcaccact ggtcacatcg tacggcaggt ttgccaatcg tgcaaccatt
     1381 ccagcaacat tcgtccatct gctcgatgcc ctgtacgttc tcctggcgat tttcggccag
     1441 atgccgcggt atcattgaca cgaacgacga cagtgtttga ctacccgtca ctgctgcgcc
     1501 gccgttctcg cctcagttga taccctcgcc aaccgccgat cccggcctgg atggaactgt
     1561 ccgtggaacc gccgaagccc cgccagcaac gccgccggcc tgcccagccc cgcagcacca
     1621 cccgcgagcc gcgcccgctg gcggccggaa cgatcacgaa ggtcacgccg acccagcgtg
     1681 accccgagcg catcagcgtc ttcatcgacg gcacgttcgt cttcgcgctg ccggacatca
     1741 tcgtcgccac gcgcggcctc aagcgtggcg ttgagctgac gctggagcag gtccgcgagc
     1801 tggcgggcat cgccgagggc gagaaagcga cggcagccgc gctgacgttc gtctcctacc
     1861 gcccgcgctc cgaacgcgag gtgcgcgacc ggctgcgccg caaagcctac gagccggccg
     1921 cgatcgacta cgcgatcgag aagatgcgcg gctggcgcta cctcgacgac accaagttcg
     1981 ccgagttctg ggtcgagagc cgtgccgaac atgctccgcg cggcaaacgc gcgctgcagt
     2041 cggagctacg cgccaaaggc gtcgaccgcg aagtcgtcga acgcgtcctt gacgggaccg
     2101 acctcgacga acgaagcggc gccctcgaga tcgcccgcaa gcgcctgcgc tcgctgtcct
     2161 cctacgacga agagacgcaa cgccgccgcc tggccgcctt cctcgctcgc cgcggctacg
     2221 gatgggatgt cgtgaaacct gttatggccg agctgttcgg cggtgacggg gatgggtgat
     2281 gggtgacgcg tcacgcgtga cgcgggaggg ggatgtcttc cccgttccca aatcccaaac
     2341 cccgaatccc gcgtcacgcg tcacccatcg cggatccaac ccaccaatga tgtatcgttc
     2401 ccggtgatcg cagtggagga gggcgcgcaa tgagtgggcg cactggcggc acggcaaccg
     2461 aggcacgcac aaccacgtcc gggatcgaga tcgcgccggt ctaccgagcc gacggtcgcg
     2521 agccggagcc ggaccccggc cagtatccct acacgcgcgg cgtctacccc gatatgtacc
     2581 gtggccggct ctggacgatg cgccagtatg ccgggttctc ctcggccgag gagaccaacc
     2641 agcgcttcaa gctgctgttg gcgcgcgggc agaccggcct ctcctgcgcc ttcgacctgc
     2701 cgacccagct cgggctggac gccgacgacc ctcgcgccga ggccgaagtc ggcatcgtcg
     2761 gcgtctcgat ctcgacgctg gacgacatgc gccggctgct ggatggcatc acgctcgacg
     2821 acgtcacgac ctcgatgacg atcaacgcgc cggcctctat cctgatgctg atgtaccaga
     2881 tcgtcggcga ggagcagggc gccaacctgg ccaggctcgg cggtacgatc cagaacgaca
     2941 tcctcaagga gtacgccgcg cggggaacct acatcttccc gccgcgccag tcaatgcggc
     3001 tggtgacgga tacct
//
LOCUS       DASUGJ010000062         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_981349, whole genome shotgun sequence.
ACCESSION   DASUGJ010000062 DASUGJ010000000
VERSION     DASUGJ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981349"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="VFV93_01495"
     CDS             complement(<1..1324)
                     /locus_tag="VFV93_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX5164041.1"
                     /translation="MRIVTGGIAQETNTFQWEPTTLTDFEKGSSRIERGQQILDLANT
                     NLIYGGIVSEALAQGVELIPTTYGHAVPGGRVSREAFESMRDEILAGIRAALPVDGVM
                     LGIHGAMALEHNDDGEGPLITAVRELVGPDVPIIAPLDLHTNLSDEMLAEANAFIGYK
                     EYPHIDMRETGQAAMQLLIRTIKGEVTPAMAHVRVPLIAPNQSMVTTWQSALKKVIDR
                     AVEIEAEPGVLSATVLGGFPFSDVPFTGVATIVVADGDKALAQRYANELAQMAWDLRE
                     EFTIHPTPIADAIAEAMAGEPGSVYVLADISDSGASGTAGDGTAVLKGLLEANARSAA
                     VAQIMDRDAVAVCIAAGVGATVTLTVGGKHDGLHGEPVEVTGMIRLIHEGRFPMGGVM
                     GSGTTASRGKTVVLEINGPGGIELQLTELRGHPNDLNFFRAFGIEPTER"
     gene            complement(1410..>2450)
                     /locus_tag="VFV93_01500"
     CDS             complement(1410..>2450)
                     /locus_tag="VFV93_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5164042.1"
                     /translation="GFERMEVGSWLNIAVSLVRAPLAVLLASWFGVEGVALAALLAAA
                     VSAELYRRTATRTGIGQVIWTFSPAHARMLALRSWPLLVNALLVNLFFRVDVFILQAV
                     KGDETLGMYDAAYKLINLVTIVPAYVTLAIFPAMAQRSADPDALDRTLRTASYFLAWI
                     AWGIVAIMTALAGTAIRVLAGSDYLPDAALLLRILIWFAPLSFLNGVIQYALVATEQQ
                     RRIVPAFVAAVTCNLAFNVALVPIYGARAAAVTTVVTELVILVAFAFVARTSALRAVT
                     VSSLLALWKPTLAGLVAAGLALLAVDRQGEAVAAPVAIGVFVLLTLALRIFGDREREL
                     VRRAFQRSAARV"
BASE COUNT          430 a          794 c          833 g          393 t
ORIGIN      
        1 ggcgctcggt cggctcgatg ccgaacgcac ggaagaagtt caggtcgttg ggatggccgc
       61 gcaactcggt cagctggagc tcgatgccgc ccgggccgtt gatctcaagg acgacggtct
      121 tgccacggct ggcggtcgtg ccggagccca tcacgccgcc catcgggaag cggccctcgt
      181 ggatcaggcg aatcatcccg gtcacctcga ccggttcgcc atgcagaccg tcgtgcttac
      241 cacccaccgt cagcgtgacg gtcgcgccga cgccggccgc gatgcagacg gcgacggcgt
      301 cgcggtccat gatctgggcc actgctgcgg aacgggcatt cgcttccagc agccccttca
      361 ggacggcggt gccgtcgccg gctgtgccgc tggcgccgga gtcggagatg tcggccagaa
      421 catagacgct gcctggttca ccggccatcg cctcggcgat cgcgtcggca attggcgtgg
      481 ggtgaatcgt gaactcctcg cgcagatccc aggccatctg cgccagctcg ttggcgtagc
      541 gctgagcgag cgccttgtcc ccatcggcga cgacgatcgt tgcgacaccg gtgaagggca
      601 catcactgaa cgggaagcca ccgaggacgg ttgccgagag gacgcccggc tccgcctcga
      661 tctcgacggc gcggtcgatg accttcttca gcgccgactg ccacgtcgtc accatcgact
      721 ggttgggcgc gatcagcggc acgcggacat gcgccatggc tggggtgacc tcgcccttga
      781 tcgtgcggat caggagctgc atggccgcct ggccggtttc tcgcatgtcg atgtggggat
      841 actccttgta gccgatgaag gcattcgcct cggccagcat ctcgtcggag aggttggtgt
      901 gcaggtcgag cggggcgatg atcggcacgt cggggccgac cagctcgcgg acggccgtga
      961 tgagcggtcc ttcgccgtcg tcattgtgct ccagcgccat tgcgccgtgg atgccgagca
     1021 tgacaccatc gaccggcagc gccgcccgaa tgccggccag gatctcatcg cgcatcgact
     1081 cgaacgcctc acggctgacg cggcccccgg gaacggcatg tccgtaggtg gtgggaatca
     1141 gctcgacgcc ctgggccagc gcttcgctga cgatgccgcc gtagatgaga ttcgtgttgg
     1201 ccaagtcaag gatctgctgc ccccgttcga tccggctcga tcccttctcg aaatcagtca
     1261 gcgtcgtcgg ctcccactgg aacgtgttgg tctcctgggc gatcccaccg gtgacgatcc
     1321 tcatctgtgc cactccctca ctcccgattg cggcttccgg tctgggcagt atcgatgctt
     1381 ggtagaaatc cggcaagatg gggagatgct cagacgcggg cggcggaacg ttggaacgcg
     1441 cggcggacga gttcgcgctc gcggtcgccg aaaatgcgca gcgcaagcgt caggagcacg
     1501 aatacgccga tcgcgaccgg cgccgcgacc gcctcgccct gccggtcgac tgccagcagg
     1561 gcaagcccgg ccgcgaccag gccggccagg gttggtttcc agagcgccag cagtgaggac
     1621 accgtgactg ctctcagcgc cgacgttcgc gcgacgaagg cgaaggccac caggatcact
     1681 agctcggtga cgaccgtcgt aacggcagcg gcgcgtgcac cgtagatcgg caccagcgcg
     1741 acgttgaagg ccaggttgca ggtgacggcg gcgacgaagg ccggaacgat tcggcgctgt
     1801 tgctcggtcg cgaccaatgc gtactggata acaccgttca ggaacgagag tggcgcgaac
     1861 cagatcagaa tacgcagcag gagggcggcg tctggcaggt agtcagagcc ggccagcaca
     1921 cggatggccg tgccggccag cgccgtcatg atggcgacga ttccccaggc gatccaggcg
     1981 aggaagtacg acgcggtcct cagcgtgcgg tctagcgcgt ccggatcggc cgatcgctgg
     2041 gccatcgccg ggaagatcgc cagcgtgacg taggcgggca cgatcgtgac gaggttgatc
     2101 agcttgtaag cggcgtcgta catgcccagc gtctcatctc ccttgacggc ctggagaatg
     2161 aagacatcga ctcggaagaa gaggttgacc agcaatgcat tgaccagcag cggccagcta
     2221 cgcaacgcga gcatccgggc atgtgccgga ctgaacgtcc agatcacctg tccgatgccg
     2281 gttcgagtgg ccgtccggcg gtagagctca gctgagacag ccgccgccag tagcgccgcc
     2341 agcgcgactc cctcgacccc gaaccagctg gcgagcagga cggccagagg cgcgcggacg
     2401 aggctgacgg cgatgttcag ccaggagccg acctccatcc gctcgaagcc
//
LOCUS       DASUGJ010000063         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_148328, whole genome shotgun sequence.
ACCESSION   DASUGJ010000063 DASUGJ010000000
VERSION     DASUGJ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148328"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..798)
                     /locus_tag="VFV93_01505"
     CDS             complement(79..798)
                     /locus_tag="VFV93_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164043.1"
                     /translation="MPQQEESIGGLAKRWLKTQLRFHGDPIKSHRDREEAEAIEQRIE
                     QKAHDDATSAVVNALMPDSWKRKLESLERANEEGRIAREQQRRAEHEARQRVPLTLSL
                     TGSVTGSVSTTVPILIEVPDEPGDPLTVDLEPLEPLEVGGHQFLGLLFAVPAYCGPGT
                     YDLTAIEARLRDDTWDPLWFQLTIDTTDDGLYWSPDYGHATITVTDTSIHLTMPMSNS
                     NGIDVNVDALVELQSGSNQSR"
     gene            complement(998..2089)
                     /locus_tag="VFV93_01510"
     CDS             complement(998..2089)
                     /locus_tag="VFV93_01510"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5164044.1"
                     /translation="MPGFFRVWFVYGSRLALAAIIAAIFIVQPQLRRPAEAATLVDGA
                     VPSVWISATGQTMSGAFLEYWIAHPEIGNPVSGAVVEGELWSQWFEYARLELEPGPFD
                     QISVNQVHRHQIGRSFAIKAGYSDALSAFKPLAEGQDRFFPETGHTLTMGFRSAYEQP
                     GVAERLGLPISEEFDIGTITYQFFEYGAFSWGPSEVAELVPLGKLDAGIQGQLGRWQP
                     QPGDAVDWDSTGLDMMALSELLPGQRTIEVDLSDFMITARVGDKVVLESIASTGVPQS
                     PTVTGHFSIYLKYRIQSLSVIGWDGNLYEAPNTPWVMYFYQDYAFHSSLWRTEYGLMD
                     SQGCVVPPMEVAKALWLWADYGTPVWIHE"
BASE COUNT          414 a          652 c          760 g          446 t
ORIGIN      
        1 agggcgatat tgcgatatcg cccgtaccgg attgaccatt gatattggtt gcatggcgta
       61 gctgtggtta cttcaacttc aacgggattg gtttgaacca gactgtaatt caacgagtgc
      121 atccacattc acatcgatgc cgttgctgtt ggacatcggc atcgtgaggt ggatcgatgt
      181 gtcggtgaca gtgatcgtgg catggccgta gtcaggcgac cagtagagac cgtcgtcggt
      241 cgtgtcgatc gtgagctgga accagagtgg gtcccaggtg tcgtcgcgca ggcgcgcttc
      301 gatcgcggtc aggtcgtacg tgccagggcc acagtaggcc gggacagcga agagcagacc
      361 gagaaactgg tgaccgccga cttcgagtgg ttccagcggt tcgaggtcta ctgtcagggg
      421 gtcgcccggt tcgtccggca cttcgatgag gatcgggacg gttgttgaga ccgaaccggt
      481 gacgctgccg gtgagcgaga gtgtcagtgg aacgcgttga cgcgcttcgt gttcggcgcg
      541 gcgttgctgc tcccgggcga tccggccctc ttcgttggcc cgttcgaggc tctccagctt
      601 gcgcttccac gagtcgggca tcagcgcgtt aacgacggca ctggtcgcgt cgtcgtgcgc
      661 cttctgctcg atgcgctgct cgatggcctc ggcttcctcg cggtcgcggt gggacttgat
      721 cggatcaccg tgaaagcgga gctgggtctt gagccagcgt ttcgcaagcc cgccgatcga
      781 ctcttcctgc tgtggcatct cgacacgcct ccctgctttc ccgacgtcta tcggtcgggg
      841 ttgcggaaac attccgttgg gtgggaagcg tgccgttcag tgtcgccggc atggagcgca
      901 acgattcctg accccgagac aatgatgcga tcgataacag tctggccgca ttgtctcgtg
      961 ggtcaggaat cggctggcga aatggacagg cagcgggcta ctcgtgaatc cagacgggag
     1021 tgccgtagtc ggcccagagc cagagcgcct tggcgacttc catcggtggg acgacacagc
     1081 cctggctgtc catcaggccg tactccgtcc gccagagcga cgagtggaag gcgtagtcct
     1141 gatagaagta catgacccag ggcgtattcg gtgcttcgta caggttaccg tcccagccga
     1201 tgacggacag cgactggatg cggtacttga gatagatcga gaagtggccg gtgacggtcg
     1261 gcgattgcgg cacgccggtc gaggcaatcg actccaggac gaccttgtct ccgacgcggg
     1321 ccgtgatcat gaagtcgctc agatcgacct caatcgtccg ctggccgggc agcagttccg
     1381 agagcgccat catgtcgaga ccggtcgagt cccagtcgac ggcgtcacct ggctggggct
     1441 gccagcgacc gagctggccc tggatgccgg cgtcgagctt gccaagcggc acgagctcgg
     1501 cgacctccga cggtccccag ctgaacgcgc cgtactcgaa gaactgatag gtgatggtgc
     1561 cgatgtcgaa ctcctcggag atcggcaagc caagccgttc agcaactccc ggctgctcgt
     1621 aggcactgcg gaagcccatc gtcagcgtgt ggccggtctc ggggaagaag cggtcctgtc
     1681 cttcggcgag cggcttgaag gcggagagcg cgtcactgta gccggccttg atcgcgaagg
     1741 aacggccgat ctggtgccga tgaacctgat tgacacttat ctggtcgaac ggtccgggct
     1801 ccagctcaag ccgggcgtac tcgaaccact gcgaccatag ctcgccttcg acaacggcgc
     1861 cactgaccgg gttgccgatc tccggatgcg cgatccagta ctccaggaac gcgcccgaca
     1921 tcgtctggcc ggttgcgctg atccagacgc tcgggacggc gccatcgacg agcgtcgcgg
     1981 cctctgcagg tctgcgcagt tgcggctgga cgatgaagat ggcggcgatg atggcggcaa
     2041 gcgcgaggcg tgatccatac acgaaccaga cgcgaaaaaa tccaggcacg ggcgaacgtc
     2101 tccgatttct cgattcgttg ccagcgcacg tcacgacagg cagtaacggc acgctgagat
     2161 gtaattgctg tctccatgat ggtctgcacg gtcggcaaac gaggatggga tgttggtccc
     2221 ggtcgagggt aggaatttca gcggccaggg ttggacgatt tcgcgtgagg ac
//
LOCUS       DASUGJ010000064         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1259653, whole genome shotgun sequence.
ACCESSION   DASUGJ010000064 DASUGJ010000000
VERSION     DASUGJ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259653"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFV93_01515"
     CDS             <1..316
                     /locus_tag="VFV93_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5164045.1"
                     /translation="FASEPVTIRNVAHIRVKETDRIAAVVTELRRMNVDVDELDDGLV
                     IRPSTPTAATIRTYDDHRMAMSFAIAATQVPGTRIADPGCVAKTVPGFWDLLFPLLGK
                     SI"
     gene            complement(345..1430)
                     /gene="aroC"
                     /locus_tag="VFV93_01520"
     CDS             complement(345..1430)
                     /gene="aroC"
                     /locus_tag="VFV93_01520"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX5164046.1"
                     /translation="MGSTFGRAFRITTWGESHGPALGVVIDGCPAGLPISDADIQHDL
                     DRRRVGQSKVTSPRNERDRVQILSGVFEGQTTGAPISLITYNADADSSKYDIIRDMFR
                     PGHADYTYWTKYGIRDHRGGGRSSARETWGRVAAGAIARGILRTAGIDVYAFTTQIGE
                     IKATTFDRCEIDQNLVRAADPDAAEAMEAAILRVKAENDSLGGVVEVHAEGVPPGLGE
                     PTVDKLDALIGQAMLSIPATKGVEIGHGFSAVSMRGSQHNDAFYIGDSGRVRNRTNHA
                     GGTLGGISNGETIIARVAVKPTSSVSQNQETVDIHGNSRTIFVEGRHDPCVVPRAVPV
                     VEAMMCLVLVDLYLLNRMSRVAWDAPV"
     gene            complement(1442..2350)
                     /locus_tag="VFV93_01525"
     CDS             complement(1442..2350)
                     /locus_tag="VFV93_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5164047.1"
                     /translation="MTEPPVTPVPDSPSMGYLAGDVRVLTPEDVRSLRMPWDGRFNER
                     EIRDILIAGPQISVFNRRTGEFLIGGPWRHRTEIATILELAATASAIDLINAFTEASL
                     LAGIKLVVASEQSERRKREFFAGASLDPIEDIIVYEMAKLRPAPPRTSGIRFEPFDPD
                     NEAQFDGLIDLDHQSFPWLWWNSADEFLEYASAPGVQIDVGRDDTGRIVAYTGTTRFR
                     SWGHLDRIAVAPDLQGRGLGKGALDWAVMTLARSGAKRVGLSTQARNTRSRRLYESYG
                     FRRSATHDYRIYARRTDGRALDDKRN"
BASE COUNT          426 a          777 c          817 g          435 t
ORIGIN      
        1 gttcgcgagc gaaccggtca ccatccgcaa tgtcgcccac attcgcgtca aggagactga
       61 ccggatcgcg gccgtcgtga ccgaactgcg ccgcatgaac gtcgatgtcg acgagctgga
      121 cgacggactc gtcatccggc cctcgacccc aacggccgca acgatccgga cctacgacga
      181 ccaccggatg gcgatgagct tcgcgatcgc ggccacccag gttcccggca cccgcatcgc
      241 cgatcccggc tgcgtcgcca agacggtgcc cggattctgg gacctgctgt ttccgttgct
      301 ggggaagagc atctgacgaa gcttcagctg tacagcactc ccgatcagac tggcgcatcc
      361 caggcgacgc ggctcatccg attcagcaga tagagatcga ccaggacgag gcacatcatc
      421 gcttcgacga ccgggacggc gcgcgggacg acacaggggt cgtggcgacc ctcgacgaag
      481 atcgtgcgcg agttgccgtg aatgtcgacc gtctcctgat tctggctgac cgaggatgtc
      541 ggcttgacgg caacgcgggc gatgattgtc tcgccgttcg agatgccgcc gagggtgccg
      601 ccggcgtgat tggtgcggtt gcggacccgg ccggagtcgc cgatatagaa ggcgtcattg
      661 tgctgcgagc cgcgcatcga gacggccgag aatccgtggc cgatctcgac gcccttcgtg
      721 gccggaatgc tcagcatcgc ctgaccgatc agtgcgtcga gcttgtcgac ggttggttcc
      781 ccgaggccgg gcggaacgcc ttcggcgtgg acctcgacga ccccgccgag gctgtcgttc
      841 tcagccttga cacgcagtat cgcagcttcc atcgcttcgg cggcgtcggg gtcggcagcc
      901 cggacgaggt tctggtcgat ctcgcaacgg tcgaatgtcg tcgccttgat ctcgccgatc
      961 tgggtcgtga aggcgtagac gtcgataccg gctgttctca agattccccg cgcgatcgcg
     1021 ccggccgcaa cccgtcccca ggtctcgcgg gcgctcgacc ggccaccacc gcggtggtcg
     1081 cggatgccgt acttggtcca gtaggtgtag tcagcgtggc cggggcggaa catgtcgcgg
     1141 atgatgtcgt acttggacga gtcggcgtcg gcgttatagg tgatcagcga gatcggcgcg
     1201 ccggtcgttt gtccctcgaa gacgccggag aggatctgga cgcggtcacg ttcgttacgc
     1261 ggcgaggtga ctttcgactg gccgacgcgg cggcggtcga ggtcgtgctg aatgtctgcg
     1321 tcactgatcg gcagcccggc cgggcaaccg tcgatgacga caccaagtgc cggaccgtgg
     1381 gattcccccc aggtcgtaat gcgaaacgcg cgtccgaacg tgctgcccat gccctaccct
     1441 gtcaatttcg cttgtcgtca agtgcgcggc cgtcggtccg gcgcgcgtag attcgatagt
     1501 catgcgtcgc cgagcgccgg aacccgtatg actcgtacag ccgacggctg cgcgtattgc
     1561 gcgcctgggt gctgagtccc acccgcttcg cgccggagcg cgccagcgtc atcacggccc
     1621 agtcgagcgc gcctttgccc agcccgcgcc cttgcaagtc gggcgcgacg gcgatccggt
     1681 cgagatgccc ccaactgcga aaacgagtcg tgccagtgta ggcgacgatc cggcctgtgt
     1741 catcccggcc gacgtcgatc tggacgccgg gcgcgctggc gtattcgagg aactcgtcgg
     1801 cactgttcca ccacaaccac ggaaacgact ggtggtcgag gtcgatcagt ccgtcgaatt
     1861 gcgcttcgtt gtccgggtcg aacggctcga agcgtatccc gctcgttctc ggcggcgccg
     1921 gccggagctt ggccatctca tagacgatga tgtcctcgat ggggtcgagc gaggcgccgg
     1981 caaagaactc gcgcttgcgc cgttcggatt gctcggaggc gacgacgagc ttgatgccag
     2041 cgagcaagga cgcctcggtg aaggcgttga tcaggtcgat ggcggacgcg gttgcggcca
     2101 gttccaggat ggtcgcgatc tcggtccgat gacgccacgg tccgccgatc agaaactcgc
     2161 ccgtgcgccg gttaaagacg gagatctgcg gacccgcgat caggatgtcg cggatctcgc
     2221 gctcgttgaa gcggccatcc cagggcattc gcagcgagcg cacatcttcc ggcgtcagga
     2281 cacgaacatc acctgccaga tagcccatcg aaggcgagtc tggaacgggc gtgacgggcg
     2341 gttcagtcat cgatccagat atcttcaagc gagagaattc caatcggtga aacgacgagg
     2401 tcgcggaccc agggctggac gaccgtatac gagacatcat ggtaaagcgg gatca
//
LOCUS       DASUGJ010000065         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_287134, whole genome shotgun sequence.
ACCESSION   DASUGJ010000065 DASUGJ010000000
VERSION     DASUGJ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287134"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1373)
                     /locus_tag="VFV93_01530"
     CDS             complement(180..1373)
                     /locus_tag="VFV93_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5164048.1"
                     /translation="MGNWDHLPIPIDVYRERRQRCRVEASARGLAGLIVFSRDPDRAG
                     NGLYLANHRPIAGSHPSMYAQRGRGYCAIVIPVDREETLLVTSPYYEPDVTIEQVAVD
                     TNLPRGLAGVLAGDSLDRGERWGLVGEEFLPVLLHRDIQAALPHCSFYFADDVLWSMR
                     TIKDELEQSILREAASIADAGAETAMQAYRIGRTENQIVADIDATLRSLGATNTSLTC
                     QSGVFRSGEPLMRPFASDRVLERGDMAQMEIRGSYKGYGFDICRSAVIGEPDETARHM
                     FETVERMLDTIIATAAPGIRAEELQHAIDQIAHDEGFDGYQSMHFGGPSTYCGHGLGV
                     ASDEPPVLFTGDRTILRPGMFLTPEPGLYRHPRGGLRLEDNILITDTGCENLNTTRRW
                     WWSED"
     gene            1440..>2150
                     /locus_tag="VFV93_01535"
     CDS             1440..>2150
                     /locus_tag="VFV93_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5164049.1"
                     /translation="MSERTIPPEWESERDLIELVRDLGRRGLADGLDASFVSGRPVDG
                     AIELMVESGLFALTLPARYGGQGRSYTALAAVSEELGRIDTSHQVSLTVHLGLTSMCI
                     LQWGNETQREQWLPDLARGERIGTFGLTEPGAGSDVGALRMRAREVESGYLLNGEKSW
                     ISSANIANLFLLFATVDPAKRHRGITAFVVPGESLGLTTTRLHGKLGVRAGDTGTVVC
                     NDVFVPRENVLGEVGEGFA"
BASE COUNT          338 a          672 c          754 g          386 t
ORIGIN      
        1 ggtctcggtt cgagcgctgg tagcaggctc gacgttgccg gcgtcgccgg gttccgtcaa
       61 gaatcgctct tcgccggggt ggtcctcgtg ctggctgggc ttctccattt atagcgtacc
      121 ttcagtgctt tccgcgccag gacatcagtc cggatggcgc acacggtgtg ccgctgggct
      181 caatcttccg accaccacca gcgacgggtc gtgttgaggt tctcgcagcc ggtgtcggtg
      241 atgaggatgt tgtcttccag gcgcaggccg ccgcgcgggt ggcggtagag gcccggctca
      301 ggtgtcagga acataccggg gcgcaaaatc gtccggtcgc cggtgaacag caccggaggc
      361 tcgtcactgg cgacgccgag gccgtgcccg cagtaggtgc tcggcccgcc gaagtgcatc
      421 gactggtagc catcgaagcc ctcgtcgtgg gcgatctggt cgatggcgtg ctggagctcc
      481 tcggcacgaa tgccgggcgc ggccgtcgcg atgatcgtgt cgagcatccg ctcgaccgtc
      541 tcgaacatgt ggcgggccgt ctcgtccggc tcgccgatga cggccgagcg gcaaatgtcg
      601 aatccatagc ccttgtacga gccgcgaatc tccatctggg ccatgtcgcc acgttcgagg
      661 acgcggtccg aggcaaacgg acgcatcagc ggctcgccgg agcggaagac gccgctttgg
      721 catgtcagcg atgtgttggt cgctcctagc gagcgtagcg tcgcgtcaat gtcggcgacg
      781 atctgattct ccgtccgacc gatcctgtac gcctgcatcg ccgtttcagc gccggcgtcg
      841 gcgatgcttg ctgcctcgcg gaggatagat tgctccagct cgtccttgat cgtgcgcatc
      901 gaccagagga catcgtcggc gaagtagaac gagcagtgtg gcagggcagc ctggatgtcg
      961 cggtggagca gcaccggcag gaactcctcg cccaccagcc cccagcgctc gccgcgatcg
     1021 aggctgtcgc cggccaggac gcccgccagc ccgcgcggca ggttcgtgtc aacggccacc
     1081 tgctcgatcg ttacgtccgg ctcgtagtag ggcgaggtca ccagcaacgt ctcctcgcga
     1141 tcgacgggga tgacgatcgc gcagtagccg cgcccgcgct gggcgtacat ggaagggtgg
     1201 gacccggcga tcgggcgatg gttggcgagg tagaggccgt tgccggcccg gtcgggatcg
     1261 cgtgagaaga cgatcagccc ggccagaccc cgcgcgcttg cctcgacccg gcaacgttgc
     1321 cggcgctcgc gatagacgtc gatcggtatc ggcagatggt cccagttacc cacgatctca
     1381 tgctccctgc tggcgagtgg ccgtctgaag ttcgtcgtat cgtagcgtag aatccgacca
     1441 tgagtgaacg cacgatcccg cctgagtggg aatccgaacg tgatctgatc gagctggtcc
     1501 gcgatctggg ccggcgtggg ctggccgacg gtcttgacgc gtcattcgtc tccggccggc
     1561 cggtcgatgg cgcgatcgag ctgatggtcg agtccgggct gttcgcgctg acgctgccgg
     1621 cacgctatgg cgggcaggga cgcagctaca cggcattggc tgccgtcagc gaggaactgg
     1681 gccggatcga cacgtcccat caggtctcgc tgaccgttca tctcgggctg acgagcatgt
     1741 gcatcctcca gtgggggaac gagacgcagc gcgagcagtg gttgccggat ctggctcgtg
     1801 gagaacggat cggcacgttc gggctgaccg aaccgggcgc cggctcggat gtcggcgcgc
     1861 tgcggatgcg ggcgcgggaa gtcgagagcg gctacctgct gaacggcgag aagtcgtgga
     1921 tcagctccgc caacatcgcc aacctgttcc tgctcttcgc gaccgtcgat ccggccaaac
     1981 ggcatcgggg catcacggcg ttcgtcgttc ccggtgagtc gctgggcctg acgacaacca
     2041 ggctgcacgg caagctcggc gttcgggcag gcgacaccgg cacggtggtc tgcaacgacg
     2101 tcttcgtgcc gcgcgagaac gtgctgggtg aggttggcga agggttcgca
//
LOCUS       DASUGJ010000066         5438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_9371, whole genome shotgun sequence.
ACCESSION   DASUGJ010000066 DASUGJ010000000
VERSION     DASUGJ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9371"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFV93_01540"
     CDS             <1..592
                     /locus_tag="VFV93_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164050.1"
                     /translation="LGEIAPLDPNVVTNYSLSTNLELKGIPRQADFVMSLLILQSAPD
                     HYYLRSTSGDSGIESWLVDGTTYLTQADGSVASIPQGSDTALFSPSLLVQTIPAISGD
                     VVGIMAGVEEVSGRQATHYTVDGEDLLDKTSWLPGDTADDIDGQVDIWIDNELHIIVR
                     QESNVTWNNRDNTQGSFVGHYEVTNIGTTEPVTAPG"
     gene            complement(589..1356)
                     /locus_tag="VFV93_01545"
     CDS             complement(589..1356)
                     /locus_tag="VFV93_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164051.1"
                     /translation="MSDEQQSAPGEQPEDIAQLETAADEAPRTSRDVLYEQIGLRLMN
                     AGVVSPAATFITDVRAAAGFRVSPREVVVAVEQIAGANEPITVEAVAGLVETMRGGRS
                     ARQQRHADAWRTLGAQLALRGMDGTPEGQRAFIGIAHAVAGRNATDALLLQIALTLAE
                     QGQKLDAETVGKVGKRLAASAADIQPEQLPGLVEREVRGLLRDARSPKTKKSRPQVRT
                     GPMSQAHNPGARKWRPGGRRRRKIQFPPKPKPEPFER"
     gene            complement(1353..2549)
                     /locus_tag="VFV93_01550"
     CDS             complement(1353..2549)
                     /locus_tag="VFV93_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5164052.1"
                     /translation="MTETAGPLSGVRVLDLSRVLAGPYCTMILGDLGADVIKVEQPGK
                     GDDTRAWGPPWAGTESAYYLTVNRNKRGITVDLKSPEGQQIIRDLAAKSDIVVENFKL
                     GTLERMGIGYEQLREVNPRLIWATVSGYGPDGPWADRPGYDFIVQGESGIMAITGEPD
                     GDPMKVGVAIVDVTTGMFTVIGILAALRARDASGAGQRVDTSLLTSAVAWLANVGQSH
                     LVSGKPAKRYGNAHASIVPYQVFKARDQYITIGVGNDRQFASLCSVLGIPEIAHDQRF
                     ATNPLRVENRDELIEILQSRFETGDAEDWLEACYRAGIPSGKINTVEQVFEHPQVLAR
                     DMLVEIEHAQAGMLKMAGIPYQLSGSPASIRLAPPTLGQHTDEVLRELGRSDDEIRSM
                     HESGAI"
     gene            complement(2691..3311)
                     /locus_tag="VFV93_01555"
     CDS             complement(2691..3311)
                     /locus_tag="VFV93_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5164053.1"
                     /translation="MYDYRPAEVPPALTRILTGLLPDDAERLVTAFRFAAAAHDGQTR
                     DEGTPFIEHPLAVADILNEELGCDDVDVLVAALNHDVLEDCGWLGEEAVAAVLGARAM
                     ALVGYVTKEQAPECEKAARDRRYLDHLRTIPREAKLLKLADRIHNLRSIPAANDPAKA
                     RRYLDVSRDEFYPLALTVDPAAAVLVADACDAIEVYLAGIDVETPE"
     gene            3406..4419
                     /locus_tag="VFV93_01560"
     CDS             3406..4419
                     /locus_tag="VFV93_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164054.1"
                     /translation="MRLGNAGQRHAGQARVSSLVRMLVTAVAGLTIFTGCATVRFGSA
                     FTETGKATHTLELTIPRSSMTEQDLARVERQLADAEKNARAGGLTTVRIDNPETIGIR
                     VINTTQDATDAGLALNSILNTLIVDETTGPVAPFQGTFQRVSEPVGGNVFELKMTVDG
                     DILYSAAAKVAPGHPQFSTAEGVREVVTIEYVVTMPGEIVETNGGKQGEGSVVWSIPL
                     QGPTQLEARSTVGKDTPWFWTILAIVASLAVVALTTALIWKLLIARQRAGRPGRRLAL
                     DASASPAMSMPPYSLPELRATLAGLVRRALHGERLNPRPPRQARQQPGSEESSRGADA
                     QGD"
     gene            4394..4702
                     /locus_tag="VFV93_01565"
     CDS             4394..4702
                     /locus_tag="VFV93_01565"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX5164055.1"
                     /translation="MALTPKETDRLLVFAAAELARKRRARGLKLNHPEAVALLTDEVF
                     EEARAGRAYADVIQHACQVLGRADVMEGVPEMIRVLHIDAMFADGTRLVTLYDPIREG
                     "
     gene            4706..5077
                     /gene="ureB"
                     /locus_tag="VFV93_01570"
     CDS             4706..5077
                     /gene="ureB"
                     /locus_tag="VFV93_01570"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX5164056.1"
                     /translation="MIPGQIIPASEPVEINPGLPVTTIRVTNTGDIPVHLTAHFHVFE
                     ANPRLRFDRRRAYGMRLDVPANGSVRVDPGETRQIALVPIGGDRVVRGFNGAVDGRLN
                     IIDVEAALNRLIERGFIHESE"
BASE COUNT          929 a         1835 c         1745 g          929 t
ORIGIN      
        1 gctcggcgag atcgcaccgc tcgacccgaa cgtcgtgacc aactactccc tctcgaccaa
       61 ccttgagttg aagggtattc ccagacaggc cgacttcgtg atgtcgctgc tgatcctgca
      121 atccgcgccg gatcactact acctgcgctc gacatccggc gattcgggga tcgagagctg
      181 gctggtcgat ggaacgacct acctgaccca ggcagacggc tcggtcgcca gcatcccgca
      241 gggcagcgac acggcgctgt tctcaccgtc gctgctggtg cagacgattc cagccatcag
      301 cggtgacgtc gtcggcatca tggccggcgt tgaagaggtc agcggccggc aggccaccca
      361 ctacacggtt gacggcgaag atctgctcga caagacaagc tggcttccag gcgacaccgc
      421 agacgacatc gacggccagg tcgacatctg gatcgacaac gagctgcaca tcatcgttcg
      481 acaggagtcc aacgtcacct ggaataaccg tgacaacacg caggggtcgt tcgtcggcca
      541 ctatgaggtg acgaatatcg ggacaaccga gccggtaacg gcgccgggct agcgctcgaa
      601 cggctccggc ttcggcttcg gcgggaactg gatcttgcgc cgtcgccgcc cgccgggccg
      661 ccacttccgc gcgccaggat tgtgtgcctg gctcatcggt ccggttcgga cctggggtcg
      721 ggacttcttc gtcttcggcg agcgggcatc acgcagcaac ccgcgcacct cacgctcgac
      781 gagacccggc aactgttccg gctggatatc ggcggcactg gctgccagcc gcttgccgac
      841 cttgccgacc gtctcagcat cgagcttctg cccctgctcg gccagcgtca acgcgatctg
      901 caacaacagc gcatcggtcg cgttgcggcc ggcgaccgcg tgggcgatgc cgatgaacgc
      961 gcgctgtccc tcgggcgtgc cgtccatacc ccgcagcgca agttgcgcgc cgagtgttcg
     1021 ccaggcgtcg gcgtgtcgct gctggcgtgc cgaacgcccg ccgcgcatgg tttcgaccag
     1081 gccggcaact gcctcaaccg tgatcggctc gttggcgccc gctatctgct cgacggcaac
     1141 gacaacctcg cgtggcgaga ccctgaaccc ggccgccgcc cgtacgtcgg tgatgaacgt
     1201 tgccgcggga ctgacgacac cggcgttcat cagccgcagc ccgatctgct cgtagagcac
     1261 atcgcgggac gtgcgtggag cctcgtcagc cgccgtctcc agctgcgcga tgtcctcagg
     1321 ctgctctcca ggcgcggact gctgctcgtc gctcaaatgg ctccactctc atgcatgctg
     1381 cgaatctcgt cgtcggaccg gccaagctcg cgcaacacct cgtcggtatg ctgccccagc
     1441 gtcggcgggg ccagccggat cgacgccgga gaaccggata gctggtaggg aatgccggcc
     1501 atcttgagca tccccgcctg ggcatgctcg atctcgacga gcatgtcacg tgccagtacc
     1561 tgcgggtgct cgaagacctg ctcgaccgtg ttgatcttgc cgctgggaat cccggcccgg
     1621 taacacgcct ccagccaatc ctcggcgtca ccggtctcga agcgcgactg gaggatctcg
     1681 atcaactcat cccggttctc gacgcgcagg ggattggttg cgaagcgctg gtcatgcgcg
     1741 atctcgggaa tcccaagcac actgcagagc gaggcaaact ggcggtcgtt cccgacgccg
     1801 atcgtaatgt actggtcgcg cgccttgaag acctgatatg gaacgatgct ggcgtgggca
     1861 ttcccgtagc gtttcgctgg tttgcccgag acgagatgac tctggccgac gttcgccagc
     1921 caggcgacgg ccgatgtcag caggctggta tcgacccgct ggccggctcc gctcgcgtcc
     1981 cgggcacgca gcgcggccag gatgccgatc acggtgaaca taccggttgt cacgtccacg
     2041 atcgcgacgc cgaccttcat cgggtcgccg tccggctcgc cggtgatcgc catgatgccg
     2101 ctctcgccct ggacgatgaa gtcgtagcca ggccggtcgg cccatggccc gtccggtcca
     2161 tagccgctga cagtcgccca gatcaggcgc gggttgacct cgcgcaactg ctcgtagccg
     2221 atgcccatcc gctccaacgt gccgagcttg aagttctcaa cgacgatatc gctcttcgcc
     2281 gccagatcgc ggatgatctg ctgcccttcc ggcgacttga ggtcgaccgt gatgccgcgc
     2341 ttgttgcgat tgacggtcag gtagtatgcg ctctctgtcc cggcccacgg cgggccccag
     2401 gcgcgggtgt cgtcaccctt gccgggctgc tcgaccttga tgacatcggc gcccaggtct
     2461 ccgaggatca tcgtgcaata cggccccgcc agcacgcgcg agaggtccag cacgcgcaca
     2521 ccgctcaagg gcccggcagt ttcggtcatc gtctcgttct cgctcttccc atggctgatt
     2581 gtgaagtctg gtccgtttcc ccgcgatttg cggcgtgaac aacggcaacc tagcctatca
     2641 caggcatctg ccggcagaga cgcgctatcg ccagcctcgt accgatccgg ctattccggc
     2701 gtttcgacat cgatgccggc gagatacacc tcgatcgcgt cgcacgcatc agcgacaagg
     2761 acggcggctg ccggatcgac ggtcagcgcg agcggataga actcgtcgcg gctgacgtcg
     2821 aggtagcggc gcgctttggc cgggtcgttc gccgccggaa tcgagcgcag attgtggatc
     2881 cggtcagcca gcttcagcag cttcgcctcg cgcggaatcg tccgcaggtg gtcgagatag
     2941 cgccggtcgc gcgccgcctt ctcacactcc ggcgcctgtt ctttggtgac atagccgacc
     3001 agcgccatcg cgcgcgcgcc cagtaccgcg gcgacggcct cttcgccgag ccagccgcaa
     3061 tcctccagca cgtcgtggtt gagcgccgcg accagtacat cgacgtcatc gcagccaagc
     3121 tcctcgttga ggatgtcggc gacggctagc ggatgctcga tgaacggcgt cccctcgtcg
     3181 cgcgtctggc cgtcgtgggc ggcggccgca aagcggaagg ctgtcacgag ccgctcggcg
     3241 tcgtcgggca ggaggccggt caggatgcgg gtcaatgccg gcggcacctc ggccgggcgg
     3301 tagtcataca ttgggcatca ccttgctggt cacgaatcgt cctgttcggc gcgggttata
     3361 gtacggtacg cagccgcggg cgtgcgcggc gacgctggag gccggatgag acttggcaac
     3421 gcagggcaac gacatgcagg gcaggcgagg gtcagttcgc tcgtccggat gctcgtgacg
     3481 gccgtcgccg ggctcacgat cttcaccggt tgcgccacgg ttcgcttcgg atcggccttc
     3541 acggaaaccg gaaaagcgac ccacacgctg gaactgacaa ttccgcgcag cagcatgacc
     3601 gagcaggacc ttgcccgcgt cgagcgacag ctcgccgatg ccgagaaaaa tgcccgcgcc
     3661 ggtggtttga cgacggtgag gattgacaac ccggaaacga tcggcatccg cgtcatcaac
     3721 acgacccagg atgcgaccga cgccgggctc gcgctcaaca gcatcttgaa cacgctgatt
     3781 gtcgatgaga cgaccggacc ggtcgccccg ttccaaggca ccttccagcg cgtcagcgag
     3841 ccggttggcg gcaacgtctt cgaactgaag atgaccgttg acggcgatat cctctacagc
     3901 gccgccgcga aggtcgcgcc cggccacccg cagttctcga cggcggaggg agttcgcgaa
     3961 gtcgtcacga tcgaatacgt cgtcacgatg ccgggcgaga tcgtcgagac aaacggcggc
     4021 aagcagggtg aaggcagcgt cgtctggtcg atcccgctcc aggggccgac ccagctcgaa
     4081 gctcgctcga cggtcggcaa ggacacacca tggttctgga cgatcctggc gatcgtcgcg
     4141 tcgctggccg tcgttgcact gacgacggcg ctgatctgga agctgctgat cgcccgccag
     4201 cgggcggggc ggcccggccg gcggctcgcg ctcgacgcct ccgcatcacc tgcgatgtcg
     4261 atgccgccgt actcgctacc ggagctacgg gcaacactgg ccggactggt gcgtcgtgcg
     4321 ctgcatggtg aacgcctgaa tccacgacca ccccggcagg ctcgccagca gcccggatcg
     4381 gaggaatcca gccgtggcgc tgacgcccaa ggagactgac cggctgctcg tgttcgctgc
     4441 cgccgaactg gcgcgcaagc gacgagcacg cgggctgaag ctcaaccacc ccgaagcggt
     4501 cgcgctgctg actgacgagg tcttcgaaga ggcccgcgcc ggccgggcct acgcggacgt
     4561 catccaacat gcctgtcagg tgctaggtcg cgccgatgtc atggaaggcg tgccggagat
     4621 gatccgtgtc ctgcacatcg acgcgatgtt tgctgacggc acccggctgg tgacgctcta
     4681 cgacccgatt cgggagggct gagtgatgat ccccggtcag atcatccctg ccagcgagcc
     4741 ggtcgagatc aaccccggcc tgccggtgac gacgatccgc gttaccaaca ccggcgacat
     4801 tcccgttcat ctgacggcgc atttccatgt cttcgaggcc aacccgcgcc ttcgcttcga
     4861 ccgccgccgc gcctacggga tgcgcctcga cgtccccgcc aacggcagcg tccgcgtcga
     4921 tcccggtgaa acgcggcaga tcgcgctcgt cccgatcggc ggagaccgcg tagtacgcgg
     4981 cttcaacggc gcggtcgacg ggcggctgaa tatcatcgat gtcgaggcag cgctcaaccg
     5041 cctgatcgag cgtgggttca ttcacgagtc agagtagcca ccccgttgtc gtcctgagca
     5101 gtccaccact atggtcctga cgagttatcg ctgcggctca ggacgacagg ggaaggcccc
     5161 ggtgcaaccc gggagcatga cggtggcgga ctacggaggc gtgagccgaa ggatctctcc
     5221 tgcgtcggcc gatcagccgg gataggagag atccttcagc tgccgcctca ggatgacaaa
     5281 agggaagtct cagttctgcc caaactccag cggcacatac gggttctcgt tcggcgggcc
     5341 gacggccacc cacatccggc cttcggaggg ttcggcgatg accgaggcga acgtgatcgt
     5401 gtcgctgtcg ggatcgtcgc ccggcatccg gcagagcg
//
LOCUS       DASUGJ010000067         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1259831, whole genome shotgun sequence.
ACCESSION   DASUGJ010000067 DASUGJ010000000
VERSION     DASUGJ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259831"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..802)
                     /locus_tag="VFV93_01575"
     CDS             complement(119..802)
                     /locus_tag="VFV93_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5164057.1"
                     /translation="MHSYGQFCPVAKAAEIFAERWTPLIMREILLGSTRFNELERGLP
                     RISRSVLSQRLKQLEHEGIIDRRVGLDGRSVEYIPTQIGQELYDVIRLLGEWGARWLN
                     EDIDPQDIDPTLLMWDMHRRIERDRLPDRQIIARFDYTGLRQKSFWLILNRHDVSVCY
                     FDPGFDVDLVATVDTQAMHRIWMGHQTFESALRTGQMRIDGPRELASQLPSWFQLSIF
                     AHVPRARAA"
     gene            1020..>2090
                     /locus_tag="VFV93_01580"
     CDS             1020..>2090
                     /locus_tag="VFV93_01580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5164058.1"
                     /translation="MVMTIERSATAQRPAPIHPGDDRFVPLAAELAAEFATRAAEHDR
                     ENTFVFENYDRMKASGYTRLAVPEELGGLGATMRQVVYAQAELAKGCAATALAVTMHH
                     YNVLAQCFRYRNNVPGAEGVLRRVANDDLILMTSGGSDGIWPSSVATRVDGGYRVNGR
                     KVFCSQAPVANVMSTMARYDDPDDGPVVLLMSVPLRSEGVEMQETWDTLGMRATSSND
                     LVFNDVFVSDAQIVTRRPWGKNDPALRNALVHFAPLGAAVYWGIAAGARDEALRVVTS
                     RPGFDGVSPTADPLVQRRVGMMQAHLQNAWWALLGSVEQLGDDYKPNPEASNIVTIAK
                     AEVVTKAVQVVDLAMETVGGAAF"
BASE COUNT          372 a          685 c          659 g          374 t
ORIGIN      
        1 cggcagcgaa ggacctctcc gcgctaccgt catgtgtggc tgacgcaggg aggtccttcg
       61 ctgccgggtg ccctctgggc ggtggatgac aggggcatgc cgaatctatg aaaggacttc
      121 aggcagcccg tgccctcggg acatgggcga agatgctcag ctggaaccag ctcggcaact
      181 ggctcgccag ctctcgtggg ccgtcgatcc gcatctgccc cgttcgcagg gcggattcga
      241 acgtctgatg ccccatccag atccggtgca ttgcctgcgt gtcgacggtc gcgaccagat
      301 cgacgtcgaa tcccggatcg aaatagcaga ccgagacgtc gtgccggttg aggatcagcc
      361 agaagctctt ctggcgcagg ccagtgtaat cgaagcgggc gatgatctgc cgatccggca
      421 accgatctcg ctcgatcctg cggtgcatgt cccacatcaa cagcgtcgga tcgatgtcct
      481 gcgggtcgat gtcctcattg agccagcgcg ctccccattc gcccagcagc ctgatgacgt
      541 cgtagagctc ctgcccgatc tgggtcggga tgtattcgac cgagcgcccg tcgagcccaa
      601 ctcgccgatc gatgatcccc tcgtgctcga gctgcttcag ccgctgcgag aggaccgagc
      661 gcgagatgcg gggcagcccg cgctccagct cgttgaatcg cgtgctcccc agcagaatct
      721 cgcgcatgat cagcggggtc caacgttcgg caaagatctc ggctgctttt gccaccgggc
      781 agaactgacc gtagctgtgc atgatgtcac ctccggtgcg agcaggtgcc gcgttcaaag
      841 gtcgtgtcac cctggatccg aacactgtcc attatcgctg agaacgcgct gattggcgaa
      901 gttcgattat cgaacttgtc ggggccgttg atcgcgctag gctgagatcg ggccgaaaca
      961 acggccagcg atggtccggc aatgtcgccg gacgcgacga tcgaaaggat cgatatccaa
     1021 tggtaatgac aattgagcga tccgcaacgg cgcaacgacc cgcgccaatc catcccggcg
     1081 acgaccgctt cgtgccgttg gccgccgagt tggccgccga gttcgcgacg cgggctgctg
     1141 agcacgaccg cgagaatacg ttcgtctttg aaaactacga ccggatgaag gcgagcggct
     1201 acacgcgcct cgccgtccca gaggagctcg gtggtcttgg cgcgacgatg cgccaggttg
     1261 tctacgccca ggccgagctg gccaagggct gtgcagcaac ggcgctcgcg gtcacgatgc
     1321 atcactacaa cgtcctcgct cagtgcttcc gttaccgcaa caacgtcccc ggagctgaag
     1381 gcgttctacg ccgcgttgcg aacgacgacc tgatcctgat gaccagcggt ggttccgacg
     1441 ggatctggcc cagctcagtc gccactcgcg tcgatggcgg ctaccgcgtc aacggccgca
     1501 aggtcttctg cagccaggcg ccggtcgcca atgtcatgtc gacgatggcc cgctacgacg
     1561 atcccgacga cgggccggtc gtcctgctga tgagcgtccc gctcaggagc gagggcgtcg
     1621 agatgcagga gacctgggac acgctcggga tgcgcgcgac ctccagcaac gacctggtct
     1681 tcaacgatgt cttcgtctct gacgctcaga tcgtgacgcg ccggccgtgg gggaagaacg
     1741 acccggccct gcgcaacgcg ctcgtccact tcgccccgct tggcgctgcc gtctactggg
     1801 gcatcgccgc cggcgctcgc gacgaagctc tcagggtcgt cacgtcccgc cccggcttcg
     1861 atggtgtctc acccaccgca gacccgctcg tgcagcgccg cgtcggcatg atgcaggccc
     1921 acctgcagaa cgcctggtgg gcgctgctgg ggtccgttga gcaactcggc gatgactaca
     1981 agcccaatcc cgaggcatcc aacatcgtta ccattgccaa ggccgaggtt gtcaccaagg
     2041 cggtccaggt cgtcgatctg gcgatggaga ctgtcggcgg cgccgccttc
//
LOCUS       DASUGJ010000068         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1120685, whole genome shotgun sequence.
ACCESSION   DASUGJ010000068 DASUGJ010000000
VERSION     DASUGJ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120685"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFV93_01585"
     CDS             <1..214
                     /locus_tag="VFV93_01585"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEX5164059.1"
                     /translation="FYFETPYKRDLDGGLKITLDAVCTLLDLDDRYIVSLTLEKRLDP
                     LHPRVELELGPAEGWQFDREYVVLEG"
     gene            complement(346..1527)
                     /locus_tag="VFV93_01590"
     CDS             complement(346..1527)
                     /locus_tag="VFV93_01590"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HEX5164060.1"
                     /translation="MDSGVGLVRRVLGWLGQPDPPLEDESQPLTVRDKSVRSLYLGRS
                     VQAWLRFEGGDPLRAAVLGVPALESFADVAALLGIDERRLVWLCYQPDDHGVDHYHRF
                     VIPKRNGEQRPISAPKADMRTAQRVIQREILAKIPVHDAATAYRPGASIVANAGHHTG
                     QAVVIRLDIADFFPTISRKRVKGMFASFGYSEGVAAVLALLTTDTPRDQTGTTSPLLG
                     RRDRVLYDDADACLPQGACTSPAIANVICGRLDARLTGLARKRGFTYTRYADDMTFSH
                     PAADAPVGRLISSAVHILGAEGFAPNASKARVTRAHQRQIVTGLLVNNGVAVPRADLR
                     RFRAILHSAERDGFEQASQTLGKDVVAYARGYIAYIQMVDPANAARIRERHPWVMQSD
                     Q"
     gene            complement(1720..2457)
                     /locus_tag="VFV93_01595"
     CDS             complement(1720..2457)
                     /locus_tag="VFV93_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5164061.1"
                     /translation="MFSFAGSLEGTIGSDDTVPDAPRPQRTQSGKRGTEEAGQTDATW
                     LSIDAACKLLGVDQSTLRRWSDSGKIPVFRTPGGHRRYNEEDLRALVRGEPRRRRVSR
                     QVLTSMSMSAYEHDYLRQAQSRPWYRAYDQRTLDELRPLGRRMVDLTIRYISSRGDRS
                     DIIAESREIGHEYGCYSARVGLSTAEALEAFLFFRTPVVQAVTHYIEDENVPTRRAAR
                     ITSELTHFLDAVLIETVSAHQEQKGGD"
     gene            2640..2960
                     /locus_tag="VFV93_01600"
     CDS             2640..2960
                     /locus_tag="VFV93_01600"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEX5164062.1"
                     /translation="MNDRQPAPLSALQAEVEQWAERYWGGEYWPPLANLARLTEEVGE
                     IARAINQAHGPKRIKSDEAAAQLAGEMGDALFALLCLANSTNVDLQAAWDATLEKYRG
                     RDEG"
     gene            complement(3103..>3473)
                     /locus_tag="VFV93_01605"
     CDS             complement(3103..>3473)
                     /locus_tag="VFV93_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan hydrolase"
                     /protein_id="HEX5164063.1"
                     /translation="GRLGYEDAQARGLLESRPFQPILHDIVRGDCVWFGETRQMVCTD
                     FMEYWRTHGLDLGDDGVSYRESLALFGYPISQPYVDPDTGLVTQYFERAVFEIHPANP
                     YPYRVLLRRLGAEVLEARGW"
BASE COUNT          592 a         1115 c         1192 g          574 t
ORIGIN      
        1 cttctacttc gagaccccct acaagcgcga cctcgatggc ggcctcaaaa tcaccctcga
       61 cgccgtctgc accctgctcg acctcgacga ccgctacatc gtctccctca ccctcgaaaa
      121 gcgcctcgat cccctccacc cccgcgtcga gctggaactc ggtccggctg aagggtggca
      181 gttcgatcgt gagtatgtgg tgcttgaagg ttgaaggacc acgacaatca atagcccaat
      241 gcgtaggggc gatgtcggtg actcgccctc aatgcaatga accaattggt caattgtgcg
      301 attggtattg tgggcgagtc accgacatcg cccctacgca ttgggctatt gatcagattg
      361 catcacccac ggatgccgct cgcgaatccg cgccgcgttg gccgggtcaa ccatctggat
      421 gtaggcgatg tagccgcgtg cgtaggcgac gacatccttg cccaatgtct ggctggcctg
      481 ctcgaagccg tcgcgttcgg cgctgtggag gatggcgcgg aagcggcgca ggtcggcgcg
      541 cgggacggcg acgccgttgt tgaccagcag gccggtcacg atctggcgct ggtgggcgcg
      601 ggtgacgcgc gccttcgatg cgttcggcgc gaacccctcc gcgccgagga tgtggacggc
      661 actggagatc agccgcccga ccggggcgtc agcggcaggg tgcgagaacg tcatgtcgtc
      721 ggcgtagcgc gtgtaagtga agccacgctt gcgggccagg ccggtcagcc gggcgtcgag
      781 acggccgcag atgacgttcg cgatcgccgg cgaggtgcag gcgccctgcg gcaggcaggc
      841 gtcggcgtcg tcgtacagca ccctgtcgcg gcgacccagc aacggagacg tggtacccgt
      901 ctggtcgcgt ggcgtgtcgg tcgtcagcag ggcgaggacg gccgcgacgc cctcgctgta
      961 gccgaacgag gcgaacatac ccttcacccg cttgcgggag atcgtgggga agaagtcggc
     1021 gatgtcgagc cggatgacga cggcctggcc ggtgtggtgg ccggcgttgg cgacgatcga
     1081 cgcgccgggc cggtaggcgg tcgccgcatc gtgaaccggg atcttcgcca ggatctcgcg
     1141 ctggataacc cgttgcgcgg tgcgcatgtc ggccttcggc gcgctgatcg ggcgttgctc
     1201 gccgttgcgc ttggggatca cgaagcggtg gtagtggtca acaccgtgat cgtccggctg
     1261 gtagcagagc cagaccagcc gccgctcgtc gatgcccaac agcgcggcga catccgcgaa
     1321 cgactccagc gccgggacac ccagcacggc cgcccgcagc gggtccccgc cctcgaagcg
     1381 cagccaggcc tggaccgagc gccccaggta gagggaccga acgctcttgt cgcgtactgt
     1441 gagcggttgc gactcgtctt caagcggagg atcgggctgc ccgagccagc cgagcacccg
     1501 tcgcacgagg cccacgccgc tgtccatcct ctccgttggg caggattcca cgaacggcgg
     1561 aggcgacctg cttcgacgaa tcacagcctg gctagccttg catgccaggt agggatggtt
     1621 agcgtcaccg cagccatggc gtagcaccac agatagattg ccaggtcgcc gcgcccgccg
     1681 tcatggttgg cgcagtctac gcggagcgga cggaacgcgt caatcgccgc ccttctgctc
     1741 ctggtgggcg ctgacggtct cgatcaggac ggcgtcgagg aaatgggtca gctcgctggt
     1801 gatccgtgcc gcgcggcgag tcgggacgtt ctcgtcctcg atgtagtggg tcacagcctg
     1861 gacaaccggc gtccggaaga agaggaacgc ctccagcgcc tcggccgtcg agaggccgac
     1921 ccgtgccgag tagcagccgt actcgtggcc gatctcgcgg ctctcggcga tgatgtcgct
     1981 gcggtcgccc cggctgctga tgtagcggat cgtgaggtcg accatccgcc ggcccagggg
     2041 gcgcagctcg tccagcgtac gctgatcgta ggcgcggtac cacgggcggc tctgcgcctg
     2101 gcgcaggtag tcgtgctcgt aggccgacat cgacatcgag gtcagcacct ggcggctgac
     2161 gcggcggcgg cgcggctcgc cacggaccag tgcccgcagg tcctcctcgt tgtagcgccg
     2221 gtggccgccg ggcgtccgga agaccggtat cttgccactg tcgctccagc ggcgtagcgt
     2281 cgattgatcg acaccgagca gcttgcaggc cgcgtcgatc gacaaccagg tggcgtccgt
     2341 ctggccggcc tcctcggttc cccgtttgcc ggattgtgtc cgttgtggtc gtggcgcatc
     2401 ggggaccgtg tcgtcgctcc ctatcgtccc ttcaagcgat ccggcaaatg agaacatcgg
     2461 cgctccagac tgctgtgtgg cggcacgatc agcgactacc gattccatgg cacgttgcgc
     2521 gggcattctc agggagattg gacagcctgt caaatactgt gggaacaatg cggttccggc
     2581 gggcgctgca tggcatgatg accgggaacc tgaaggcggg acgagacgag gagcgattga
     2641 tgaacgatcg gcagccggcg ccgctgagtg cgctccaggc ggaggtcgag caatgggctg
     2701 agcgctactg gggcggcgaa tactggccgc cgctggcgaa cctggcccgg ctgaccgagg
     2761 aagtcggcga aatcgcccgc gccatcaacc aggcacacgg cccgaagcgg atcaagtccg
     2821 acgaagccgc cgcccagctg gccggcgaga tgggcgacgc cctcttcgcc ctcctctgcc
     2881 tcgccaacag caccaacgtc gacctccagg ccgcctggga cgcgacgctg gagaagtacc
     2941 ggggcagaga tgagggatag acaaccagcc aatcaatgcg taggggcgat gtcggtgacc
     3001 ctcaatctca accgcgcaat tgaccaattg gttcattgca ttgagggcga gtcaccgaca
     3061 tcgcctctac gcattgattg gctgattggt gatgggactg gatcaccaac cccgcgcctc
     3121 cagcacctcc gcgccaagcc ggcgcagcag gacacggtac ggataggggt tcgcgggatg
     3181 gatctcgaag acggcgcgtt cgaagtactg ggtaaccagg ccggtgtcgg gatcgacgta
     3241 gggctggctg atcgggtagc cgaagagtgc cagtgactca cggtagctga cgccgtcgtc
     3301 gccgaggtcg agcccgtgtg tgcgccagta ctccatgaag tcggtacaga ccatctggcg
     3361 cgtctcaccg aaccagacgc agtcgccccg gacaatgtcg tgcaggatcg gctggaacgg
     3421 ccggctctcc agcagccccc gcgcctgagc gtcctcgtag ccgagccggc cga
//
LOCUS       DASUGJ010000069         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_982325, whole genome shotgun sequence.
ACCESSION   DASUGJ010000069 DASUGJ010000000
VERSION     DASUGJ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982325"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..>2531)
                     /locus_tag="VFV93_01610"
     CDS             complement(11..>2531)
                     /locus_tag="VFV93_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HEX5164064.1"
                     /translation="VRMEDRFPIIDILDQTPPIPENAQWAMFLRNHDELTLEMVTDEE
                     RDYMYRSYAQDPAARINLGIRRRLAPLMHNNRRRIELMNGLLFSLPGTPILYYGDEIG
                     MGDNIFLGDRNGVRTPMQWSGDRNAGFSTANSQRLYFPVITDPEYHYSTVNVETQQAN
                     PQSLLWWMKRLIALRKRYRAFGRGSIEFLHPENRKVLAFVRKYEEETILVIANLSRFV
                     QAVELDLSEYRGFSPIEMFGSNEFPSIGDLPYFVTLGPHSFYWFSLEPQRVTGDRISA
                     GDTETQVPATAVSGAWTALFRGDARTRLERMLPRYLADRRWFRGKSRRITRVRINDVV
                     PVSYDESSSAVIVVQVDYSEGDPEQYVLALSFATGDRATDLMTYQQHAILMRLTAGDE
                     EGVVYDALWDQGFNDALLGFISRRRKLRGEHGTISGATTRAWREHAPVPDEGPLNATT
                     VRGEQSNSSVTYADRFILKLFRKLEPGFNPDLEIGRFLTEESDFEQIAPVVGSLEYQP
                     AGGGNGNQPIVLGILQRYVPNEGDAWTYTLDMVEDFIEDSLAGRPDIDAGPLSIGALL
                     DLAESPVPEEAHERIGRYLGDAHLLGQRTAELHMALADSDKADFKPEASTMLYQRSLY
                     QSMRGLTNKTIQALRKRIPAMPEAVAAEAEQIIGWQNAINGRFRDIVNARLTARRTRF
                     HGDYHLGQVLYTGRDFVIIDFEGEPARTINERRQKRSPLRDVAGMLRSFHYAAYTALF
                     DQQERGLIGADDPLPERWVRYWYQWASVSFLRAYFETVDNASFLPAGREEREMLLDIF
                     LLDKAVYELGYELNNRPDWVHIPLRGITDLMSGRVTAEAAG"
BASE COUNT          402 a          826 c          845 g          458 t
ORIGIN      
        1 tcggtgcgcg tcagcctgcc gcctccgccg taactcgccc gctcatcagg tccgtaatgc
       61 cgcgcagggg gatgtggacc cagtcggggc ggttgttgag ttcgtagccg agttcgtaga
      121 ccgccttgtc gagcaggaag atgtcgagca gcatctcccg ctcctcgcgg ccggccggca
      181 ggaagctggc gttgtcgacg gtctcgaagt aggcgcgcag gaagctgacg gacgcccact
      241 ggtaccagta gcgcacccag cgttcgggca gcgggtcgtc ggcgccgatc agtccccgtt
      301 cctgctggtc gaacagcgcc gtgtaggccg cgtagtggaa cgagcgcagc ataccggcga
      361 catcgcgcag gggcgagcgc ttctggcggc gctcgttgat cgtccgggcc ggctcgccct
      421 cgaagtcgat gatgacgaag tcgcggccgg tgtagagcac ctggccgagg tggtagtcgc
      481 cgtggaaacg ggtccgtcgc gccgtcaacc gcgcattgac gatgtcgcgg aatcgaccat
      541 tgatcgcgtt ctgccagcca atgatctgct cggcttcggc cgccaccgcc tccggcatgg
      601 cgggtatccg cttgcggagc gcctgaatcg tcttgttcgt cagtccgcgc atcgactggt
      661 acagcgaacg ctgatagagc atcgtcgaag cttccggctt gaagtccgcc ttgtcgctgt
      721 cggccaacgc catgtgcagt tcggccgttc gctggccgag caggtgcgcg tcgccaagat
      781 agcggccgat tcgttcgtgg gcctcctccg ggactgggct ttcggcaagg tcgagcagcg
      841 cgccgatcga gagtggtccg gcgtcgatgt ccggccgccc ggccagcgag tcttcgatga
      901 aatcctcgac catgtcgagc gtgtaggtcc aggcgtcgcc ctcgttggga acgtaacgct
      961 ggaggatgcc caggacgatc ggctggttgc cgttgccgcc gccggccggc tggtactcca
     1021 gactgccgac gaccggcgcg atctgctcga agtcgctctc ctcggtcagg aagcggccga
     1081 tctccaggtc ggggttgaag cccggctcca gcttgcggaa cagcttgagg atgaagcggt
     1141 cggcatacgt aactgacgaa ttcgactgct cgccccggac ggtcgtcgcg ttgagcggcc
     1201 cctcgtcggg caccggtgcg tgctcgcgcc aggcgcgggt cgtcgcgccg gagatcgtgc
     1261 cgtgctcgcc gcgcagcttg cgccggcggc tgatgaatcc gagcagtgcg tcgttgaagc
     1321 cctggtccca gagcgcgtcg tagacgacac cttcctcgtc gccggccgtc agccgcatca
     1381 ggatcgcgtg ctgctggtag gtcatcaggt cggtcgcccg gtcgccggtc gcgaacgaca
     1441 gcgccaggac gtactgttcc gggtcgccct ccgagtagtc gacctgaacg acgatcacgg
     1501 ccgaactcga ttcgtcgtaa ctgaccggca cgacatcatt gatccgcacc ctcgtgatcc
     1561 gccgcgactt ccctcggaac cagcgccgat cggccagata gcgcggcagc attcgctcca
     1621 gtcgcgtccg ggcatccccg cggaacaacg ccgtccacgc cccgctgacc gccgtggccg
     1681 gcacctgtgt ttcggtgtcg ccggccgaga tgcggtcgcc ggtcacccgc tgcggctcca
     1741 gtgagaacca gtagaacgag tgcgggccga gcgtcacgaa atagggcaga tcaccaatcg
     1801 atgggaactc gttgctgccg aacatctcga tcgggctgaa gccgcgatac tccgatagat
     1861 cgagctcaac ggcctggacg aagcgcgaca ggttggcgat gacgaggatc gtctcctcct
     1921 cgtacttgcg cacgaacgcc aataccttgc ggttctccgg atgcaggaat tcgatgctgc
     1981 cacgcccgaa ggcccggtag cgcttgcgca gcgcgatcag ccgcttcatc caccacagca
     2041 gcgactgcgg gttggcttgc tgcgtctcga cgttgacggt cgagtagtgg tactccgggt
     2101 cggtgatgac cgggaagtag agccgctggg agttggcggt cgagaacccc gcgttgcggt
     2161 cgccgctcca ctgcatcggc gtccggacgc cgttgcggtc gccgaggaag atgttgtcgc
     2221 ccatcccgat ctcatcgccg tagtacagga tcggcgtccc cggcagcgag aagagcaacc
     2281 cgttcatcag ctcaatccgg cgccggttgt tgtgcatcag cggcgccagc cgccgccgga
     2341 tgccgaggtt gatccgcgcg gccgggtcct gcgcgtagga gcggtacatg taatcccgct
     2401 cctcgtcagt caccatctcc agcgtcagct cgtcgtggtt gcgcagaaac atcgcccact
     2461 gcgcgttctc cgggatcggc ggcgtctggt cgaggatgtc gatgatcggg aagcggtcct
     2521 ccatccggac c
//
LOCUS       DASUGJ010000070         2873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1398685, whole genome shotgun sequence.
ACCESSION   DASUGJ010000070 DASUGJ010000000
VERSION     DASUGJ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398685"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VFV93_01615"
     CDS             <1..1585
                     /locus_tag="VFV93_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5164065.1"
                     /translation="LWKPGLGKTERVLYRLPDLEAAPRGARVYVCEGEKDADALAGLG
                     LIATTNFGGAGNWNNDYSQQLLAFDVVILPDNDEPGRAFALDLVDQIATCTPTRIVDL
                     PGLPPKGDVSDWLAAGGTVAELEQLVAQTPLAEIRPPVSAGPRLITRKLSDVAPEQVT
                     WLWEGWLPRGKLSLLGGHPGDGKSTLTTALAATLSTGGRWPDGSQAPVGSTLFLLAED
                     ALGDTVRPRLDQHGADVERIEAVEMIEHEDGREQLFSLSEHLEMLEERIVEMGAALLV
                     IDPLSDFLTSRNRNDDGDIRDVLTPLGKLAERCNVAILGIMHIGKPGAVRKRPLQTFM
                     GSTAFGAVARQALLVHPIPESDRKLLAVIKSNVAVFPRPLEWSRPLDSAIVWHGQSQH
                     SVEALLGGGGVDAPPTQREIAGQFLREVLADGPLLTRSVHELARERGISETTLQRESQ
                     SMGITYRKIGAGRDAPWYTGLPETDWTAFAGRITNRQDGTPGVLCRNPCDTDDGVLGT
                     PSNEWKMMPHHRPGEYPHH"
     gene            complement(1744..1953)
                     /locus_tag="VFV93_01620"
     CDS             complement(1744..1953)
                     /locus_tag="VFV93_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164066.1"
                     /translation="MIFGVILIGVGLYLVLFRKSSAARADRRVASGSWRPRKPFPIIS
                     ERDHIVGGLLLMALGTMGMLILWLD"
     gene            complement(2003..2128)
                     /locus_tag="VFV93_01625"
     CDS             complement(2003..2128)
                     /locus_tag="VFV93_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164067.1"
                     /translation="MTFRELPRWMRIWIVLAFILIVVGLVTCVALGTTSSMDVDY"
     gene            2490..>2873
                     /locus_tag="VFV93_01630"
     CDS             2490..>2873
                     /locus_tag="VFV93_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164068.1"
                     /translation="MTSMQHPKWRVLLLGGVSGTGKSTVARAMIRALGVTWVQVDDLR
                     LTLERSLPALPPGMEPLAFFHQPDVWSRSAEAICDGLIRVGDVMAPSIEAVTENHDDQ
                     GDPAIVEGDGILPTLFARPSIQQRVA"
BASE COUNT          530 a          940 c          925 g          478 t
ORIGIN      
        1 gctctggaag ccggggctgg gcaagaccga gcgggtgctc taccggctgc cggacctgga
       61 ggccgcgccg cgtggcgctc gcgtctacgt ctgcgagggg gagaaggacg ccgacgcgct
      121 cgccggcctg gggctgatcg cgacgaccaa cttcggcggg gccggcaact ggaacaacga
      181 ctacagccag caactactcg cgttcgatgt cgtcatcctg ccggacaacg acgagccggg
      241 ccgcgcgttc gcgctcgatc tggtcgatca gatcgcgacc tgcaccccca cccggatcgt
      301 tgacttgccg gggctaccgc cgaagggcga tgtctccgac tggctggcag cgggtgggac
      361 ggttgccgag ctggagcaac tcgtcgcgca gacaccgctg gccgagatcc ggccgcccgt
      421 ctcagccggc ccacggctga tcacccgcaa gctctcggac gtcgcgcccg aacaggtgac
      481 gtggctctgg gagggctggc tgccgcgcgg caagctctcg ctgctcggcg gccaccccgg
      541 cgacggcaaa tcgacgctga cgacggcgct cgcggcaacc ctctcgaccg gcgggcgctg
      601 gccggacggc agccaggcgc cggttggctc gaccctcttc ctgctggccg aggacgcgct
      661 gggcgacacc gtccgccccc ggctggacca gcacggagcc gatgtcgagc ggatcgaggc
      721 ggtcgagatg atcgagcacg aggacggacg cgagcagctc ttctcgctca gcgagcacct
      781 ggagatgctg gaggaacgca tcgtcgagat gggcgcggcg ttgctggtga tcgacccgct
      841 ctccgacttc ctgacgagcc gcaaccgcaa cgacgacggc gacatccgcg atgtactgac
      901 accgctcggc aagctggccg aacgctgcaa cgtcgccatc ctcgggatca tgcacatcgg
      961 caagccgggg gcggtccgca agcggccgtt gcagacgttc atgggctcga cggccttcgg
     1021 cgcggtcgcc cgccaggcgc tgctcgtcca cccgattccg gagagtgatc gcaagctgct
     1081 ggcagtcatc aagagcaacg tggccgtctt cccgcgcccg ctggagtgga gccgcccgct
     1141 cgacagcgcc atcgtctggc acggccagtc gcaacacagc gtcgaggccc tgctcggtgg
     1201 tggtggcgtt gacgcacccc cgacccagcg agagatcgcc ggccagttcc tgcgggaggt
     1261 gcttgcggac gggccgctgc tgacacgctc ggtccacgaa ctcgcccgag agcgcggcat
     1321 ctcggagacg acgctgcaac gggaaagtca gtcgatgggg atcacctatc gcaagatcgg
     1381 agcgggccgt gacgctccct ggtacaccgg gctgcctgag accgactgga cggctttcgc
     1441 cggcaggatc acgaaccgac aggacggcac ccccggagta ctctgtcgga atccctgtga
     1501 tacagatgac ggagtactcg gaactccctc caacgaatgg aagatgatgc ctcatcatcg
     1561 ccctggcgaa tatccacacc actagccgct gatgatgaga cgaagcttgc cggcgacgaa
     1621 gcgagaacga ggtcgagacg attctttctc tcgctggcac gctctcatcc gtcgaaccgg
     1681 accagtattc agtagtctgt catctgtatc acagctgttc tggcagagta gtcgttcgtt
     1741 gtgtcaatcg agccagagga tcagcattcc catcgtgccg agcgccatca ggagcaatcc
     1801 gccgacgatg tgatcgcgtt cagagatgat cgggaacggc ttacgcggtc gccacgatcc
     1861 gctcgccaca cgccgatctg cacgggcggc actgctcttc cgaaacaaca caagatagag
     1921 tcccacaccg attagtatca ccccaaagat catcagcgca tgctcacaat cgtctgtttt
     1981 cgaaagacag tcagcagccg catcaataat cgacatccat gctcgacgtc gtgccgaggg
     2041 caacacaggt aaccagacca accacgatca ggatgaacgc cagcacgatc cagatacgca
     2101 tccagcgtgg cagctcacgg aacgtcattg ttcagccgtc ctccgatcgt tgcagattac
     2161 acttcaccca tgatgcgcat gtgtcatcga tcggacggga cgtgatgcga ccaccaaatc
     2221 gggagccgtc gtggtttggc tgccagtgta tgggatgtct cgccctgtcg gcagtcgcgg
     2281 cattgctgac cacctgcatg tgcgtgctgc ttgccggtct ggacggcgat ctcgtatccg
     2341 gtcccggtcg aacacctcct gaagaggcgc cggatctcga ctggatatcg tgggtgttct
     2401 ggggagtggc agcaacctgc gccatcgttg gcgcggggct gtgggtcaca tggcggcagc
     2461 gttgacggtc tgctgaaaga gctggaatcg tgacgagcat gcagcacccg aagtggcgcg
     2521 tcctgctcct cggtggagtc tccggcaccg gcaagtcgac ggttgccaga gccatgatcc
     2581 gggccctcgg cgtcacctgg gtccaggtgg acgacctacg gctgacgctc gaacggagcc
     2641 tgccggcgct cccgccgggg atggagccgc tcgccttctt ccaccagccg gatgtctggt
     2701 cgcgatccgc cgaagcgatc tgtgacggct tgatacgtgt cggcgacgtg atggccccat
     2761 cgatcgaggc cgtcaccgag aaccacgacg atcaaggtga cccggcgatc gtcgagggcg
     2821 acggaattct gccgacgctg ttcgcacgac cgtcgatcca gcaacgcgtt gcc
//
LOCUS       DASUGJ010000071         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1260031, whole genome shotgun sequence.
ACCESSION   DASUGJ010000071 DASUGJ010000000
VERSION     DASUGJ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260031"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFV93_01635"
     CDS             <1..366
                     /locus_tag="VFV93_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164069.1"
                     /translation="VAAGQNDRAQAAQLARESLALSDELGDRLMVAEGLERLARLAIV
                     ERATTGQAVQLLGAAAALREQINAPRSPSGRAEYERTLAAARRQIGETAFAARWDAGH
                     HVTLEEAVATALAGNTDSG"
     gene            complement(546..1238)
                     /locus_tag="VFV93_01640"
     CDS             complement(546..1238)
                     /locus_tag="VFV93_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5164070.1"
                     /translation="MSARIMIVEDEPQMRRAVGAGLRANGFAVLAVESGEDALDSVPL
                     TRPDVILLDLTLPGLDGLEIVRQIREWSATPIIVLSAREGEQDKVRALDLGADDYLTK
                     PFGMEELLARIRVALRHAAHQAADSGPVFSDSGLVIDFAGRGVTVDDVEIHLTPTEFD
                     LLRELALNAGKVMTHHHLLGRVWGPASVNDTQYLRTYVNQLRKKIERDPNQPRRIINE
                     PGIGYRYRRGGT"
     gene            complement(1235..2884)
                     /locus_tag="VFV93_01645"
     CDS             complement(1235..2884)
                     /locus_tag="VFV93_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5164071.1"
                     /translation="MGGWAAVLWGYDRRAVWLTDIRAAPARRVVDMGAAVLLLGLATV
                     VMLAVRTHLNILSVALIFLVLVVIIATFADRWVSVAASFVAFVLFDYFFVLPYYSLDV
                     AAADHVLALVVFLGTAAVISQLVYRVRMRTIEALARGRQMEILFDLSQALIADVTLAG
                     MLSAIAERVADVLGTAACAILLVDEQGQPVVHAAVGPPPPIEDRDHQVVALWALEHRR
                     PAGLGQRRSRHVLPHGSRSQTRRSPLPSRDGASLYVPIGVPARVSGLLYVSHVGSRQQ
                     FSSEDQHLLQLFANQIALALERVRLGEEATRAAVLARSDELKSALLSAVSHDLRTPLA
                     SIKASATSLLQPDIDWSPNDQRDLLIAIDEETDRLTRLVRNLLDLTRIEAGALRPQTE
                     WNDPEEVIRETVARAGGIYPRHEIRVQLPAAVPLVMFDYVEIAQVLINLIENAGKFAP
                     PETTIDISCRLVPGAIEFAVADRGPGIPAGEEEAIFGKFYRIGRSGGPQGAGIGLSVC
                     RGIVEAHGGRIWAEPREGGGAVFRFALPRDADRLATCESVP"
BASE COUNT          537 a         1070 c         1002 g          473 t
ORIGIN      
        1 gtcgccgccg gccagaacga ccgggcccaa gccgcccaac tcgctcgcga aagcctcgcc
       61 ctcagtgatg agcttggcga tcggctgatg gtcgcggaag ggctggaacg gctggcccgg
      121 ctggcgatcg tcgagcgagc aacaaccggc caggccgtcc aattgctcgg cgcagcagca
      181 gcactgcgcg aacagatcaa cgccccccgc tcgccctccg gccgggccga gtacgagcgg
      241 acactcgccg cagcccgtcg ccagatcggc gaaacagcat tcgcggccag atgggacgca
      301 ggccaccacg tgacactcga agaggccgtc gccaccgcgc tggcaggcaa tacggattct
      361 ggttgatgac gacagcatcc gcaacgcgaa tgcgcataac aatcaattcc caatcgcgta
      421 ggggcgattt cgcaaaatcg cccttctccg agcgccgggt ggctgaaagg gcgatattgc
      481 gatatcgccc gtaccggatt gactattgtg gacttggatt atgtcacctg tcggccggaa
      541 caggctcatg tgccgccgcg gcggtagcga tagccgatgc ccggttcgtt gatgatccgt
      601 cggggctggt tcgggtcgcg ttcgatcttc ttgcggagct ggttgacgta ggtgcgcagg
      661 tactgggtgt cgttgaccga ggccggcccc cagacgcggc cgagcaggtg gtgatgggtc
      721 atcaccttgc cggcgttgag ggccagctca cgcagcaggt caaactcggt cggcgtcaga
      781 tggatctcga cgtcatcgac cgtcacgccc cggccggcga agtcgatgac gaggccgctg
      841 tcgctgaaga ccggtccgct gtccgccgcc tgatgggccg cgtgccgcag ggcaacccga
      901 atccgcgcca gcagctcctc catcccgaac ggcttggtca ggtagtcgtc cgcgccgagg
      961 tcgagcgccc ggaccttgtc ctgctcccct tctcgcgccg agagaacgat gatcggcgtg
     1021 gccgaccact cgcggatctg gcgcacgatc tccagcccat ccagccccgg cagcgtcaga
     1081 tcgagcagga tgacgtccgg ccgcgtcagc gggaccgagt cgagcgcatc ctcgccggat
     1141 tcgaccgcca gaacggcaaa cccgttcgcg cgtagacccg ccccaaccgc ccggcgcatc
     1201 tgtggctcgt cctcgacgat catgatcctg gcgctcatgg cacgctctcg caggtggcta
     1261 accgatcggc gtctcgtggc agcgcgaagc ggaagacggc cccgccgccc tcccgcggct
     1321 cggcccagat tctgccacca tgcgcctcga cgatcccgcg acagacgctc agaccgatcc
     1381 cggcgccctg tgggcctccg ctccgcccaa tcctgtagaa cttgccgaag atcgcctcct
     1441 cctcgccagc cgggataccc ggcccgcggt cggcaaccgc aaattcgatc gcgccgggaa
     1501 ccagccggca cgagatgtcg attgtcgttt ccggcggggc gaatttcccg gcgttctcga
     1561 tcaggttgat gagcacctgc gcgatttcga cgtagtcgaa catgaccagc gggacggcag
     1621 ccggtagctg gacacgaatc tcgtggcgcg ggtagatccc gccggcccgc gcgaccgtct
     1681 cacgaatgac ctcctcggga tcgttccact cggtttgtgg ccggagcgcg ccggcctcga
     1741 tgcgcgtcag gtcaagcagg ttgcgcacga gccgcgtcag ccggtcggtc tcctcatcga
     1801 tcgcgatcag caggtcgcgc tggtcgttcg gcgaccagtc gatgtccggc tggagcaggc
     1861 tcgtcgccga ggccttgatc gacgccagcg gcgtccgcag atcgtgtgag acggccgaca
     1921 ggagggcaga cttcagctcg tccgagcggg ccaggacggc ggcacgggtg gcctcctcgc
     1981 ccagccggac tcgctccagc gcgagcgcga tctggtttgc gaatagttgc agcaggtgct
     2041 ggtcctcgct ggagaactgt tgccggctcc cgacatggct gacatacagc agcccgctga
     2101 cccgggctgg gacgccgatc gggacgtaga gactggcccc gtcccgcgac ggaagcggtg
     2161 agcggcgcgt ttgcgaccgg ctcccgtgcg gcaggacatg ccggctgcgc cgctgcccaa
     2221 gtccggccgg ccggcgatgc tccagcgccc agagcgcgac gacctgatgg tcgcgatctt
     2281 ctatcggcgg tggcggcccg acggcggcgt ggacgaccgg ctgcccctgc tcatcgacca
     2341 gcaggatcgc gcaggcggcc gtcccgagca cgtcggccac ccgctccgcg atcgcgctga
     2401 gcatcccggc cagcgtcacg tcggcgatca gcgcctgcga gagatcgaag aggatctcca
     2461 tctggcgacc gcgcgcgagc gcctctatcg tccgcatccg gacccgatag accaactggc
     2521 tgatcaccgc ggccgtccca agaaagacga ccagcgccag gacatggtcg gccgccgcga
     2581 catccagact gtaatagggc agcacgaaga agtagtcgaa cagcacaaag gcgacgaacg
     2641 acgcggcaac gctcacccag cggtcggcaa acgtcgcgat gatgacgacc agcaccagga
     2701 agatcagcgc gacactgagg atgttgaggt gggtccggac cgccagcatg acgacggttg
     2761 ccaggccaag cagcagcacc gccgcgccca tgtcgacgac acgcctcgcc ggcgccgcgc
     2821 ggatgtcagt gagccagacc gcccgacgat cgtatcccca gagcaccgct gcccagccgc
     2881 ccatgcgatc gcaatccagc cgtcattcta gcgcggcatt caagaggaca ttcttgtcac
     2941 ctcaccgtgg aggggccgat cctgtatcgg ccagtgccgt gaggcacccg ctaaccacca
     3001 tgtgcgtttc gaacggttcc ccaacgcctg cgcccgtggg gtaaacctgt ctgcgggtgg
     3061 ccgtgcgcct cacggcgctg gc
//
LOCUS       DASUGJ010000072         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1816297, whole genome shotgun sequence.
ACCESSION   DASUGJ010000072 DASUGJ010000000
VERSION     DASUGJ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816297"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFV93_01650"
     CDS             <1..632
                     /locus_tag="VFV93_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164072.1"
                     /translation="FSRILYGARYSLVIGVAAVFVSFAIGAAIGMVAGFYPKLDTPIM
                     RLIDVLMAFPGILLAIAIVAALGPGLRNVIIAIAVNEIPGFARLTRSMVLSLRERDYV
                     LAARVVGGTDLTIIRRHILVNLVSPIIVFASLRVSTSILVAATLSFLGLGIQPPTPEW
                     GAMVSTARQYLSTAPHTFFYPTMAIFITVIAFNLLGDSLRDVLDPTLRE"
     gene            733..1221
                     /locus_tag="VFV93_01655"
     CDS             733..1221
                     /locus_tag="VFV93_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX5164073.1"
                     /translation="MGIVIPFEQLGPLGNDLREQRRRIVFTNGHFDLLHVGHLRYLQA
                     ARGLGDVLVVGVNDDAVTTLRKGRDRPILPEGERAELLAGLMCVDYATIFHEETAELA
                     VRLLRPDVYVKGGDYGPGGAELPEARVVAEYGGETVILPLEPGRSTSAIVAAIRAGES
                     LT"
     gene            1218..>2513
                     /locus_tag="VFV93_01660"
     CDS             1218..>2513
                     /locus_tag="VFV93_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX5164074.1"
                     /translation="MSQPGRPDPDGTGYDIQETQAFDLIERSGRAGDTAPLPIVPAID
                     HAPRRQPRAGQIVRERRPESVQAEPVAEAVAEPVDTEAERGRSTAQALTQGIAFAAAM
                     LMIGNILSRVLGLAREQIAAGFFGTSNDIAAFTIADNIHTMLFDLVISGMMQAALIPV
                     LSEYATPERREEFRRITGALLVLAGVVVGTVVTFLMIFAPSAVGVMTSLGGGEEAHGA
                     ETFALTVELTRWILPAVLMLAFSTILMSSLYALQRFTRPALSLSLRNVAIVGTTLALG
                     WTSFGVRSMALGIVLGAMLLIVVQLPGLRDAMPKPNFHFTHPAIRRIGVLYIPIFIGL
                     FANTAALIVDRNLAWGVDNVHALGAMRYATTLNQMVLGLVAAAISLASLPALSRFWAA
                     GDVENYWITLMRGLRMITVLAIPATLGLAVLAWPVVRLLF"
BASE COUNT          357 a          841 c          879 g          436 t
ORIGIN      
        1 tcttcagccg gatcctctac ggcgcacgct actcgctggt gatcggcgtc gcggccgtct
       61 tcgtcagctt cgcaatcggg gcggcgatcg ggatggtggc cgggttctac ccgaagctcg
      121 acacgccgat catgcgcctg atcgatgtcc tgatggcctt ccctggcatc ctgctggcga
      181 tcgcgatcgt cgccgcgctc ggtccgggct tacgcaacgt catcatcgcg atcgccgtca
      241 acgagatccc cggcttcgcg cggctgacgc gctcgatggt cctctcgctg cgcgagcgcg
      301 actacgtgct ggcggcgcgg gtcgtcggcg ggacggacct gacgatcatc cgccggcaca
      361 ttctggtgaa cctggtctcg ccgatcatcg tcttcgcctc gctgcgggtc tcgacgtcga
      421 tcctggtcgc ggcgacgctc tcgttcctgg gtctcggcat ccagccgccg acgccggaat
      481 ggggcgcgat ggtcagcacg gcccgccagt acctttcgac cgcgccgcac accttcttct
      541 accccacgat ggcgatcttc atcaccgtca tcgccttcaa cctgctcggc gactccctcc
      601 gcgatgtcct cgatcccact ctacgcgagt gatcccgcgg atcaatgtcc gcgggctaaa
      661 cgtaccgaac ggcgactggc gcggcttgat acgatcacag ccggaattgc gcgcggcggg
      721 ggagtcgtcg gagtggggat cgtcataccg ttcgagcaac tggggccgct gggcaacgac
      781 ctgcgtgagc agcggcggcg gatcgtcttc accaacggcc acttcgacct gctgcacgtc
      841 gggcatctgc gctacctgca agcggcccgc gggctcggcg acgtgctggt cgtcggggtg
      901 aacgacgacg ctgtgacgac gctccggaag gggcgggacc ggccgatcct gccggagggc
      961 gagcgagccg agctgctggc cgggctgatg tgcgtcgact acgccaccat cttccacgag
     1021 gaaaccgccg agctggcggt gcgactgcta cggccggatg tctatgtcaa gggcggcgac
     1081 tacgggccgg gcggcgcgga gttgccggag gcgcgggtgg tcgcggagta cggcggcgag
     1141 acggtcatcc tgccgctgga gccgggccgc tcgaccagcg cgatcgtggc ggcgatccgg
     1201 gccggagagt cgctgacgtg agccagccag gccggccgga ccccgatgga accggatacg
     1261 acatccagga gacgcaggca ttcgacctca tcgagcgcag cggacgagcc ggcgacacgg
     1321 cgccattgcc gatcgtgccg gcgatcgacc acgcgccacg ccgccagcca cgggctgggc
     1381 agatcgtccg cgaacgacgg ccggagtcgg tacaggcaga gccggtcgcc gaggcggtcg
     1441 cggagccagt cgacactgaa gcggagcgtg gcaggtcgac ggcacaggca ctcacgcagg
     1501 ggatcgcctt cgcggcggcg atgctgatga tcggcaatat cctctcgcgg gtgctggggc
     1561 tggcgcgtga gcagatcgcg gccggcttct tcggcaccag caacgacatc gccgccttca
     1621 cgatcgccga caacatccac acgatgctgt tcgacctcgt catcagcggc atgatgcagg
     1681 cggcgctgat cccggtgttg agcgagtacg cgacgccgga gcggcgcgag gagttccgca
     1741 ggatcaccgg cgcgctgctg gtgttggcgg gcgtcgttgt tggcacggtc gtcacctttc
     1801 tgatgatctt cgcgccgtcg gcggtcggcg tgatgacctc gctgggtggc ggcgaggagg
     1861 cgcacggggc cgagacgttc gcgctgacgg ttgagctgac ccgctggatc ctgccggccg
     1921 tcctgatgct ggccttctcg acgatcctga tgtcgtcgct ctacgcgctg cagcgcttca
     1981 cgcgaccggc gctgtcgctc tcactgcgca acgtcgcgat cgtcgggaca acgctggcgc
     2041 tgggctggac ctcgttcggc gtgcgctcga tggcgctggg gatcgtcctc ggggcgatgc
     2101 tgctgatcgt cgtccagctg ccggggctgc gcgacgcgat gccgaagccg aacttccact
     2161 tcacccaccc ggccatccgg cgcatcggcg tcctctacat cccgatcttc atcgggctgt
     2221 tcgccaacac ggcagcgctg atcgtcgacc gcaacctggc ctggggcgtc gacaacgtcc
     2281 acgcgctggg cgcgatgcgc tacgcgacga cgctgaacca gatggtgctg gggctggtgg
     2341 cggctgccat ctcgctggcc tcgctgccgg ccctctcgcg cttctgggcg gcgggggatg
     2401 tcgagaacta ctggatcacg ctgatgcgcg ggctgcggat gatcaccgtt ctggcgatcc
     2461 cggcgacgct cgggctggcg gtgttggcat ggccggtcgt ccggttgctg ttc
//
LOCUS       DASUGJ010000073         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_565491, whole genome shotgun sequence.
ACCESSION   DASUGJ010000073 DASUGJ010000000
VERSION     DASUGJ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565491"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFV93_01665"
     CDS             <1..198
                     /locus_tag="VFV93_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX5164075.1"
                     /translation="PRRDPETADEYQPDIATQFTPSTGVPAAIATITDLYRHTRYSGE
                     PATEQEAARVLDAWRVVEQSG"
     gene            264..1445
                     /gene="hemW"
                     /locus_tag="VFV93_01670"
     CDS             264..1445
                     /gene="hemW"
                     /locus_tag="VFV93_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX5164076.1"
                     /translation="MTASSSLPVPLPAGATPGLYVHVPFCRHICPYCDFNTYAGQAGL
                     IPAYVAAVISEMRLAPAGQPPTAAPTLFFGGGTPSLLSSEQVGAIVSAARGLFGLADD
                     AEITLEANPESLDAAYLTGLREAGINRLSIGVQSQQRAGLRVLGRGHTADRAERALAM
                     ARDAGFDNVSLDFIFGWPGQREADWQADLATILAWQPEHVSLYSLIVEPGTPMHAAVD
                     RGILRALDDDLVAGMYERAVAVLAGAGWEHYEIANFAREPHYRSRHNQLYWQAGSYHG
                     FGAGAHGTLDGVRSSNLLLPAQYIAAVGEGRRPVAISETLSNETLMGETMMLGLRLLV
                     DGVSATDFSSRHGQSLTDRYAAQIDRFTSIGMLEWHTPDRLRLTGRGALLANDVCVAF
                     L"
     gene            complement(1516..>2754)
                     /locus_tag="VFV93_01675"
     CDS             complement(1516..>2754)
                     /locus_tag="VFV93_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5164077.1"
                     /translation="EASNSSYGGDLLAKIGAIEVVDPLTVAFTLPQPYADFAVDVATY
                     GIIAEHIWSDVAPADVINDPGSTGDDATRVVGTGPFVFKEWVPNDHAAATANAEYWGG
                     APHLAEYIYKIVPDSTAAIQQLKTGEIDWFSEVPGPSVQELEDADIDVAVSETLGFSF
                     YGYNLDPEHTTLFQEPEVRQALFYALDREAMIDAIEEGYGTVAIGTMPTLSWAFDPDA
                     IENQYAYDPDKARELLDAAGWIEGSDGVREKDGQRLSFSMYADSGSPLSDAYLTSMQE
                     FWAQVGAEMTPQLEPFPSLVERISVTYDFDMFLIGFSWGPSPDQSAMFDCDSYGAGFN
                     VMRYCNEQVDELLEEALAEPDMAKRTELYSDYQNLLLEDLPAAILSFPQAIDGYSPRV
                     HNLYPNAINARFNAETWWVE"
BASE COUNT          442 a          927 c          893 g          492 t
ORIGIN      
        1 ccccgccgcg acccggaaac ggccgatgaa taccagcccg atatcgccac gcaatttacg
       61 ccgtcaacag gagtaccggc tgcaatcgcg acgatcaccg atctgtaccg ccacacgcgc
      121 tacagtggag aaccggccac cgagcaggag gcggcccgcg tcctcgacgc ctggcgcgtg
      181 gtggaacagt ccgggtagcg ttcgcgcgtt tcgtatcatt gagcttgtta cgtctaccac
      241 gtgagcttga acaggaatcc cgcttgaccg catccagttc cctgcctgtg ccgctgccgg
      301 ccggcgcaac tcccggcctg tatgtccatg tcccgttctg ccggcacatc tgcccctact
      361 gcgacttcaa cacctacgcc ggccaggcag ggctgattcc cgcgtacgtg gcggctgtca
      421 tcagcgagat gcggctggca ccagccgggc agccgccaac cgccgcgccg acgctcttct
      481 tcggcggtgg aacaccatcg ttgctgtcgt cggagcaggt tggcgcgatc gtcagcgccg
      541 cgcgaggcct gttcggactg gctgacgacg cagagatcac gctggaggcg aaccctgagt
      601 cgctcgacgc cgcctacctg actggattgc gcgaggccgg catcaaccgg ctctcgatcg
      661 gtgtccagtc gcagcagcgg gcggggctgc gggtgctggg gcgtggtcat accgccgacc
      721 gggccgagcg cgcgcttgcg atggcccgcg acgccggctt cgacaatgtc agcctcgact
      781 tcatcttcgg ctggccgggt cagcgagagg ccgactggca agccgacctg gcgacgattc
      841 tcgcctggca gccggagcac gtctcgctct actcattgat cgtcgaaccg ggaacgccga
      901 tgcacgccgc cgtcgatcgc ggcatcctgc gggcgctgga cgatgatctg gttgccggga
      961 tgtacgagcg ggcggttgcg gtgctggccg gcgccggttg ggagcattac gagatcgcca
     1021 acttcgcccg cgagccgcac taccgctcac gccacaacca gctctactgg caggccggtt
     1081 cgtatcacgg cttcggagcc ggcgcccatg ggacgctcga cggggttcgc tccagcaatc
     1141 tcctcttgcc ggcgcagtac atcgcagccg tcggcgaggg ccgccggccg gtcgccatca
     1201 gcgaaacgct ctcgaacgag acgctgatgg gcgagacgat gatgctcggc ctccggctgc
     1261 tggtcgacgg cgtctccgcc accgacttct ccagccgcca cggccagtca ctcaccgacc
     1321 gctacgctgc ccagatcgat cgcttcacct cgatcggcat gctcgaatgg cacacccccg
     1381 accgcctgcg gctcaccggc cgcggcgcgt tgctggcgaa cgatgtctgc gtggcgtttc
     1441 tgtgacggcc ctcacccacc aaccccctct cccaatcctg gacgaggggc ttggtgggtg
     1501 agggctggtg atggcttact ccacccacca cgtttccgcg ttgaagcggg cgttgatcgc
     1561 gttggggtag aggttgtgga cgcgcgggct gtagccgtcg atggcctgcg ggaagctgag
     1621 gatggcggcc ggcaggtctt cgagcagcag gttctgatag tcgctgtaga gctcggttcg
     1681 cttggccatg tccggctcgg ccagcgcttc ttccagcagc tcgtcgacct gctcgttgca
     1741 gtagcgcatg acgttgaagc cggcgccgta cgagtcgcag tcgaacatcg ccgactggtc
     1801 cggtgacgga ccccagctga agccgatcag gaacatgtcg aagtcgtagg tgacggagat
     1861 gcgctcgacc agcgacggga acggttccaa ctgaggggtc atctctgccc cgacctgtgc
     1921 ccagaactcc tgcatcgacg tcaggtacgc gtcagagagc gggctaccgg agtcggcata
     1981 catcgagaac gacagacgct ggccgtcctt ctcgcggact ccgtccgagc cttcgatcca
     2041 gccggccgcg tcgagcagct cgcgtgcctt gtcgggatcg taggcgtact ggttctcgat
     2101 cgcatccgga tcgaaggccc acgagagcgt cggcatcgtc ccgatcgcga ccgtgccgta
     2161 gccctcctcg atcgcgtcga tcatcgcctc gcggtcgagc gcgtagaaca gcgcctggcg
     2221 cacttccggc tcctggaaga gcgtcgtgtg ttctggatct aggttgtagc cgtagaacga
     2281 gaaacccagc gtttcggaga cggcgacgtc gatgtcggcg tcttccagtt cctggaccga
     2341 cggccccggc acttcgctga accagtcgat ctcgccggtc ttcagctgct ggatagccgc
     2401 cgtcgaatcc gggacgatct tgtagatgta ctcggccaga tgcggcgcgc cgccccagta
     2461 ctcggcatta gccgtcgccg ccgcgtggtc gttggggacc cactccttga agacgaacgg
     2521 cccggtcccg acaacgcgcg tcgcgtcatc gccggtcgaa cctggatcgt tgatgacgtc
     2581 ggccggcgcg acatcgctcc agatgtgttc ggcgatgatg ccgtaggtgg cgacgtcgac
     2641 ggcgaagtcc gcgtacggct gcggcagcgt gaaggcgacc gtcagcgggt caaccacctc
     2701 gatcgcgccg atcttggcca gcaggtcgcc gccgtacgac gagttgctgg cctc
//
LOCUS       DASUGJ010000074        10633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_704379, whole genome shotgun sequence.
ACCESSION   DASUGJ010000074 DASUGJ010000000
VERSION     DASUGJ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704379"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6031)
                     /locus_tag="VFV93_01680"
     CDS             complement(<1..6031)
                     /locus_tag="VFV93_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX5164078.1"
                     /translation="MNRTNVNRINRIATIVTALALLVGLLVPFVSPASVNAQGAAPIL
                     VIVDAASSNKFGSFIEEMLNAEGINDFEVRPLTALNSTLLAGREAVILAESSPTSAQI
                     TALDNYVNAGGGLIAMRPATGLSATLGVSLQSGTTTDGYMRITDTAIGGGLYTDTMQF
                     HGVAKQFQLAGAREVARLYSTRTGATLFPAVTINDRGAGKAAMFAYDLPQSVVLTRQG
                     NPANANVDADGDGRFRTIDLYRNWADLERSRVPQADMQQRLLVRLLEEVGAFPIPRLW
                     YFPNSAPTVIVATGDNHGPNPYYGPLIDSMLAQGVPMTIFGGGATTEETLAWMAQGVD
                     FATHPYVDCGYECGYAEAYSSFENLFGFAPRTVRTHQIRGFGWTDAASIESQFGVELD
                     FNYYQWGSWVTNSEGPALGYIGGSGQPMRFIDANGNLLPIYQQHTNLVDEFLSPGVGV
                     ANLNAEQAEAASRTTIDDLVDLYYEPLVTQYHGDYYHNETRAWDDESTRYALQRGAFA
                     LNADDWLTFIQQRAATAVTNTSWQNNTLTFDMSAGGSNQSTLIPLQIGARLLDTIRIG
                     GSAITYTTRIVHGRAYASIPVSTAHYVITYTSDTTAPTVTGTTPLSGATSIPLTSPIS
                     VTFSEVRQRSVTEAAFSIAPAATGVVAWNSAGTTMTFTPTALLSPTTTYTATVTTGAR
                     DLAGNPLASTTSWSFTTATATPPPTITGVSPISGTIEGGTVVTITGANFTGATAVKFG
                     TVAAASITVNSDTQITATTAAHPAGAVHITITTAAGTSALTANDQFTFIDGSSTVSIL
                     GIDPATGSANGGTVVTITGTGFQTGMSVTVGGVAATQVTVSSTTRLKATTPAHAPGVA
                     NVVVTAPSNASATLLNGFTLVACPARCVTYSSTQYFGAGSFDAGVIASETTDGEVMLA
                     PATGAEFSGNAIPATWSSTAWQTGGQSNVSNGELRADGTMVATNDFFGPGRSIEFVAT
                     FQSGALQQHIGFATNLRDAPWAIFSTRADGAVLYARTNAGNGPLDTQLSSSWLGAPHR
                     FRIDWTTSSIVFSIDGVQVATHNVAITANMRPVISDGTAGGAILPVDWLRVDPYAASG
                     TFDSRIVDAGGSAAWGGMFWSANVPSGTSVSVSVRTGNTPLPDGTWTAFSTLSSSGSS
                     VSATSRYLQYRVSLATTSSAQSPAFRDVTIAYSVSGPLSPTIDDLNPSSGSTHGGTVV
                     TITGSNYAAGATVTFGGVAATSVTVVNSTTITATTPASAAGPVDVVVTNSNGGSGTLP
                     ASFTFVIPPPVVSAIDPTSGTRFGGTQITITGTNFDGATEVTFGTATATFTLESPTTI
                     TAIAPSHAAAVVDVRVRTGGGLSATSIAGQYTYLDIPPPPTLAAIDAGTGPTAGGATV
                     TLTGTNFVSGSTVTFDGIPATSINVVSNTRITLVTPAHAVGSVTVVVDTPDDQSASLS
                     NGYRYVDCSEGCVGQTSADDFRSGASLNTFTDEEADGSVILAPAAGGTFSGSALPAGW
                     SASPWSSGGSGSVSSGSVHVDSALVATGSYYGAGGVLEFVATFKSTPSQYIGFGQTLL
                     SAPWAIFSTGRSGTSLYVTSSSPLASQETNLGSNWLGSPHQFRIEWQANRVIYAIDGT
                     VVATHNVAIGGQMRPVISDWTLDTLTVDVDWMRLSPYQASGSFTSRVLDAGGAATWTS
                     ANWSASTPAGTTLAVSVRSGTTPVPDGSWSSFSTLGSNGAAIANTGRYLQYRLDLTGD
                     GAGITPRLRDILFTYTLSGALGPAVSAVTPASGPTGGGTSVTITGSAFATGATVSIGG
                     VAATAVTVVNATTITATTPAHAAGTADVTVTNPDGQRGILTNGYTFIPPVPAITGLTP
                     SRGALAGGTVVTISGTAFTGATVVTFGGAAATFTVDSDTKITATSPSHAAGIVDVVVT
                     TAGGTSSTAPAARFEYFAQSVVSVTSIDPDLGSAAGGTNITISGSGFQTNATVSLGGT
                     TATNVVVVNATMITATTAAHSAGLVNVAVTNPDGGQGTYSNGYRYVACTDGC"
     gene            6789..8276
                     /locus_tag="VFV93_01685"
     CDS             6789..8276
                     /locus_tag="VFV93_01685"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164079.1"
                     /translation="MVIDVRRQPVVPDLNSVRQFSVLPVVIGGVLFIAYAILYLQLRQ
                     TLLPMMAVCGLCLSVLIVIRPAIGLIAVIACAAVVRVTIGTGAGTDSVIVASLASAFA
                     LVAGWTVHRMLHRRSLILLPRFVAIPGALFIVFTIFSLMWGRITLDPRVNFYPAFLRV
                     QLAAAALSIVSVGIVFVGADLLRHRSTRGALIGFYIAAGFLALPLRSFSLGAIGEQLS
                     FINTRGLFGLWFVAICSAFGVAGGSRPLVVRLALIGGAVGWVMMAFFREGSWVSGWLP
                     AVIAMVITTLLARPKIGVPLLIAALIGVTAYYSVVYDSLVTQQEKEGSLGGEFGRIEL
                     WQRNLEVIGPRVWLGTGPAGYALYYVTLVPNEAMSTHSNYIDVLAQNGAPGLLSLLGL
                     LGGIGIMGVQTLRRVADDDDRALSCAVLGGLPAVMVAIWFGDWLIPFVYNQTIAGFDH
                     SVYSWLMFATLAGLYAQYRTTSEQSGPELRFRVVLHPNVARALPE"
     gene            8573..10039
                     /locus_tag="VFV93_01690"
     CDS             8573..10039
                     /locus_tag="VFV93_01690"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX5164080.1"
                     /translation="MLALLPANRVAASEPMNWSEPEQIGTNLSTSWFPEIFADAAGTI
                     RVVWDAGLERSEGSLATGRSGVMLSQSVDSGWSEPSEILLGQAGYVTRPIIVTDSSYA
                     YLLFRTSRMYVSRAPLTSDLQNAHSWSTPYPLSSDESYWGQIVALPDGTVVTVFSQMA
                     SVQVAGNTERRMALFMRRSVDHGQTWGYEERLSDTSERTARSSLVLAPDGRTLLLAWD
                     EGYDALTGGGDPHAISLLASRDGGATWGSPNTILGEYDQSVLATNGTTSILVYRSTTT
                     DELMFRRSEDLGETWSEQAPIPGVTARPYTSTHHFDKLSLAVDGDGRILLSYIGQPSD
                     NPDQLSVMVATFFAAEWTTPQMVATPEGLPEYPRICVGLGNQVRLVYFVRDSEASDEQ
                     RRTLWAVSATSSAQPATPVPYQAVSVETPVVTSVFAATPVPRPTVPSAPSPVSPGTVR
                     TDSPQTIVIQPVLLLIASILASVVAVAGVGRLWTEIRN"
     gene            10287..>10633
                     /locus_tag="VFV93_01695"
     CDS             10287..>10633
                     /locus_tag="VFV93_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5164081.1"
                     /translation="MQSAEATVARNSLFSVAGQIALKLLSFAFVILVVRQLGETDYGR
                     YSAALAFVGIFSVFADMGVASYAVREIARSRDRAPSLLVNMAVIRVGLAVLVIACTVG
                     GAWLVGYDRSMIGL"
BASE COUNT         1931 a         3192 c         3322 g         2188 t
ORIGIN      
        1 cacagccgtc ggtgcaggcg acatagcggt agccgttgct gtaggtgcct tggccaccgt
       61 ccggattggt gacggcgaca ttgaccaacc cggcactgtg cgcggcggtc gtggcagtga
      121 tcatcgtggc attgacaacg acgacgttcg tagccgtcgt gccgcccagg ctgacggttg
      181 cgttcgtctg gaagcccgag ccgctgatgg tgatgttcgt gccgccggcc gccgatccca
      241 ggtcgggatc gatcgatgtc acgctgacga ctgactgggc gaagtactcg aagcgtgcgg
      301 ccggagccgt gctggaggtt ccacccgccg ttgtcaccac aacatcgacg ataccggccg
      361 catggctcgg gctggtcgcg gtgatcttgg tgtcggagtc aaccgtgaag gtggcggctg
      421 cgccgccgaa cgtgacaacc gtcgcgccag tgaaggccgt gccgctgatc gtgacgaccg
      481 ttcccccagc gagtgcgccc cggctaggtg tcaggccagt gatggccggc accggcggga
      541 tgaaggtgta gccgttggtg aggatgccac gctggccatc cgggttcgtc accgtcacat
      601 ccgccgtgcc ggcggcgtgg gctggggtcg tcgcggtgat cgtcgtggca ttgacgacgg
      661 tgaccgcggt ggccgccacg ccgccgatgg agaccgtcgc gccggtggca aacgccgagc
      721 cggtgatcgt cacactcgtg ccaccgccgg tcggtcccga agccggcgta acggcgctga
      781 cggccgggcc gagcgccccg gacagtgtgt aggtgaacag gatgtcgcgc agtcgcggtg
      841 tgatgcccgc gccatccccg gtcagatcga ggcggtactg gaggtagcga ccggtgttgg
      901 cgatggcggc accgttgcta ccgagggtgc tgaagctgct ccagctgcca tcgggaaccg
      961 gcgtcgtgcc gctccggaca ctgaccgcaa gcgtcgtccc agccggagtg ctggccgacc
     1021 agttggcact cgtccaggtg gcggccccac cggcgtccag gacgcgcgag gtgaagctgc
     1081 cgcttgcctg gtacggactc agccgcatcc agtcgacatc aactgtcagg gtgtcgagtg
     1141 tccagtcact gatgactggc cgcatctgtc cgccgatcgc gacgttgtgc gtcgccacga
     1201 ccgtcccatc aatcgcgtag atgacacggt ttgcttgcca ttcgatcctg aactgatgcg
     1261 ggctaccgag ccagttgcta ccgagattgg tttcctggga ggcaagcgga ctcgacgagg
     1321 tgacgtacag cgacgttccg gatcgtccgg tgctgaagat agcccacggc gccgacaaga
     1381 gagtctggcc gaagccgatg tactgtgagg gagtactctt gaacgttgcg acgaattcga
     1441 gcacgcctcc ggcaccgtag tagctccccg ttgcgaccag tgccgaatcg acatggacgc
     1501 tgccggagct gacgctgccg ctgccaccgc tcgaccatgg acttgccgac catccggcag
     1561 gcagtgcgct tccactgaag gtgccgccgg ccgctggggc gaggatgacc gagccgtcgg
     1621 cctcttcatc ggtaaacgta ttcaaggatg ctccgctgcg gaagtcgtcc gcgctggtct
     1681 ggccgacaca gccctcactg cagtccacat agcggtagcc gttgctgagg ctcgcactct
     1741 gatcatcagg cgtgtcgacc acaactgtga cactgccaac tgcatgcgct ggcgtcacaa
     1801 gcgtgatccg cgtattgctg acaacgttga tcgacgtcgc tggaatgccg tcaaaggtca
     1861 ccgtagaacc actgacgaag ttcgttccag tgagggtaac cgttgcgcca ccggctgttg
     1921 gaccggtccc agcatcgatc gctgccagcg tcggaggtgg tgggatatcc agatacgtgt
     1981 attggccggc gatcgaagtc gctgagagtc cgccgcctgt ccgcacgcgg acgtcaacga
     2041 cagccgcagc atgggacggc gcgatcgcgg tgatcgtggt cggcgattcg agggtgaagg
     2101 tcgccgttgc cgtcccgaat gtcacttcgg ttgcgccatc gaagttggtg ccggtgatgg
     2161 tgatctgcgt cccgccgaat cttgtgccgg acgttgggtc gatggcagac acaaccggcg
     2221 gtgggatgac gaacgtgaag ctcgccggca gggtgccgga gccaccattc gagttcgtga
     2281 caacgacatc gaccggccca gccgcgctgg caggtgttgt ggcagtgatc gtcgtgctat
     2341 tgacgacggt gactgaggtg gctgccacgc cgccgaaggt cacggttgcg ccggccgcgt
     2401 agttcgatcc ggtgatcgtc acgaccgtgc caccgtgggt tgatccggac gacggattca
     2461 gatcgtcgat tgtcggtgac agcgggccgc tgaccgagta ggcgattgtc acatctcgga
     2521 aggcaggtga ttgtgccgac gatgtcgtcg cgagactcac ccgatactgg agatagcgcg
     2581 aggtagcgct gaccgatgaa ccagaactgc tgagtgtgct gaacgctgtc caggttccgt
     2641 caggcagcgg ggtgtttccg gtgcgcacac tgaccgacac gctcgtgccg ctcggcacgt
     2701 tggcggacca gaacatgcct ccccaggctg ccgacccacc cgcgtcaacg atgcgcgagt
     2761 cgaacgtgcc ggatgctgca tatggatcga cccgtaacca gtcgaccggc aggatggcgc
     2821 ctccggcagt gccatcgctg atgaccggac gcatgttggc cgtgatggca acattgtggg
     2881 tcgccacctg aacgccatcg atcgagaaga cgatacttga agtcgtccag tcaatgcgga
     2941 agcgatgcgg agctccgagc cagctgctcg acagttgggt gtccagtggc ccgttgccgg
     3001 cgttggtacg cgcatacaga acagcgccgt ccgcacgggt gctgaagata gcccacggag
     3061 cgtctctcag gtttgtagcg aacccgatgt gctgctgaag tgcaccactc tggaaggtag
     3121 caacgaattc aatcgagcgt ccaggaccaa agaaatcgtt ggttgcgacc atcgtcccgt
     3181 cggcacgtag ttcgccgttc gagacgtttg actgcccacc ggtctgccag gcggtgctgc
     3241 tccatgtggc gggaattgcg ttgccgctga attcagcacc agttgcgggc gcgagcatca
     3301 cctcgccatc cgttgtctcg gaggcgatga cacccgcgtc gaatgatcct gcgccgaagt
     3361 actgggtcga actgtaggtg acgcatctcg ccgggcaggc gacgagtgtg aacccgttca
     3421 gcaatgtcgc cgacgcgttc gacggcgccg tcacgacgac atttgcgacg cccggcgcat
     3481 gtgccggtgt cgttgccttc aggcgcgttg tactggacac ggtgacctgc gtcgccgcga
     3541 ctcccccaac cgtgacgctc atgccggtct ggaatccggt acctgtgatc gtgacgacag
     3601 tgccgccgtt cgccgatcca gtcgccggat cgataccaag gatactgact gtcgatgatc
     3661 cgtcgatgaa cgtgaactgg tcgttcgccg tcaacgccga ggtgccggcg gccgttgtaa
     3721 tggtgatgtg gaccgcaccg gcgggatgag cggctgttgt agcagtgatc tgcgtgtcgg
     3781 aattcaccgt aatggaggcc gcagccaccg tgccgaactt cacggcagtc gcgccggtga
     3841 aattggctcc ggtgattgtc acaaccgtgc caccctcgat cgtgcccgaa atcgggctga
     3901 caccggttat cgtcggaggt ggtgtggccg tcgcagtcgt gaacgaccag cttgtcgtac
     3961 tcgcgagcgg gttccccgcc agatcgcgtg ctcccgtcgt gaccgtcgcc gtgtatgtcg
     4021 tcgtgggtga gagaagtgct gtcggcgtga acgtcatcgt cgtaccagcg ctgttccacg
     4081 cgaccacacc ggttgccgca ggtgcgatgg agaacgctgc ctccgttacc gaccgctgcc
     4141 tgacctccga gaaggtgacg ctgatcggcg acgtgagcgg gatgctcgtc gcgccggaga
     4201 gcggcgttgt cccggtgacg gtcggcgcag tcgtgtccga tgtataggtg atgacgtagt
     4261 gcgccgtcga gactgggatc gacgcgtacg ctcgcccgtg gacgatgcgc gtcgtgtatg
     4321 tgatcgcgga tccgccgatt cggatagtgt ccagcagacg cgcaccaatc tggagcggga
     4381 tcagcgtcga ctgattcgaa ccgccagcgc tcatatcgaa tgtaagcgtg ttgttctgcc
     4441 aacttgtgtt ggtcaccgct gtagcagcgc gctgctggat aaaggtcagc caatcatcgg
     4501 cgttgagcgc gaaggcgcca cgctgcaagg cgtagcgtgt agactcgtcg tcccatgccc
     4561 gggtctcgtt gtggtagtag tcaccatgat attgagttac cagcggctca tagtagaggt
     4621 cgaccagatc atcgatcgtc gtccgtgagg ccgcctccgc ttgctcggca ttcagattgg
     4681 cgactccaac gcccggagac aggaattcat cgaccagatt ggtgtgctgc tggtagatag
     4741 gcagcagatt accgttggca tcgatgaagc gcatcggctg cccgctgccg ccaatgtagc
     4801 cgagagctgg accctcggag ttggtcaccc agcttcccca ttgatagtag ttgaaatcca
     4861 gttcgacgcc gaactgcgac tcgatgctcg ccgcatcggt ccagccaaat ccacggatct
     4921 gatgggtccg gacggtacgc ggagcgaagc cgaagagatt ctcgaacgaa ctgtaggctt
     4981 cggcgtaccc gcattcatag ccgcagtcga catacggatg cgtcgcgaag tcaaccccct
     5041 gtgccatcca ggccagggtt tcctccgtgg ttgcgcctcc accgaagatc gtcatcggca
     5101 caccctgcgc cagcatggaa tcaatgagcg gaccgtagta gggattcggg ccgtgattgt
     5161 cgcccgtcgc gacaatcacc gtcggagcgc tgttcgggaa gtaccagagg cgtgggatcg
     5221 gaaaggctcc gacttcttcg agcaggcgaa caaggagccg ctgctgcata tctgcctgcg
     5281 gtactcgcga acgctccagg tcagcccaat tgcggtagag gtcgatcgtc cgaaagcgtc
     5341 cgtcaccgtc agcatcaaca tttgcgttcg ccggattgcc ctgacgggtc aggacgacgc
     5401 tctgcggcag atcgtaggca aacatcgcgg ccttaccagc accccggtcg ttgatcgtta
     5461 ccgctggaaa gagcgtcgca cccgttcggg tcgagtacag tcgtgcgacc tcacgtgcac
     5521 cagcaagctg gaattgcttc gcgacgccat gaaattgcat cgtgtcggta tacagtccac
     5581 caccgatagc ggtgtccgtg atccgcatgt acccgtcggt ggtcgttcca gactgcaggg
     5641 agacgccaag cgtggctgaa agtccggtcg ccggacgcat cgcaatcagg ccacctccgg
     5701 cgttgacata gttgtccagc gcggtaattt gagcacttgt cggcgacgat tcggccaaga
     5761 tgaccgcttc acgtcccgcg aggagcgtcg agttcagggc agtcaacggg cggacttcga
     5821 aatcgttgat gccctccgcg ttcagcatct cttcaatgaa ggagccgaac ttattgctcg
     5881 atgccgcgtc gacgatgacg aggatcggcg ctgcgccctg cgcattcacc gatgccggcg
     5941 acacaaacgg aacaagcaga ccaaccagaa gtgccagtgc tgtgacgatc gtagcgatcc
     6001 ggttgatgcg gttgacgttg gtccgattca tgtgtctcta ctcctcaaag ctggaaacta
     6061 cggccgtggc gaacgacaca ccagacaatc tgagttctcg aagtgtttgg ggatgaaaat
     6121 gagactggct tcacaggttg acgcaatctg ggcggttgcc atgcgagaag ccatcgcagc
     6181 cagggaacat tgcgtcggtc ttcatatctc gtaccctttc ccaacccacg cgcgcccggg
     6241 gcatgccgct ccaggctggt cttaggctaa tctccgtgca cgccgctcag cccgacctcc
     6301 ggtcgcaggt atctgacact tccttcattg tcgacagcga tggcattcgt atgacgggtt
     6361 tgtcaccagc gaatggaggc ccgtttgggc gttgtcacac gcggcaacga cgatgagttc
     6421 aacgtcgttg atcactgtcg gtaaggggcg gaatgtcggg tacgttgcct gtcgctcgct
     6481 caaccagtgc tgacgcggaa cggcaggcgg tcattgcgca gcgcgatctg gtccacgcac
     6541 catcgattgg cgtaggtccg accgtctcgc tgccagtcgc tgctgtcccg gaactcattg
     6601 gcacgagtct gccagcggct ttcgataacg agggcattgt ggcgagcagt ggactcaagg
     6661 gcatcgccgc tgggtccgac cgcacgtcct cgtcgatgat tcggccgaac gttgggtgca
     6721 atttgtctgt ggtcgccggt aagttctggt tagccaatga gtgaaatacg aaaacagagg
     6781 aagatgacgt ggtaatcgac gtgcgacgcc aacctgtggt gccggacctc aactcagttc
     6841 ggcaattcag cgtgctccca gtcgtgatcg ggggcgtgct cttcatcgcg tatgccattc
     6901 tctatctgca gttgcgccag acgctcctgc cgatgatggc ggtctgtggt ctgtgcctga
     6961 gcgtcctgat tgtgatccgc ccggcgattg gactgatcgc cgtcatcgca tgtgccgctg
     7021 ttgtgcgcgt aacgatcggc accggagctg gcaccgatag cgtcatcgtc gcaagtctcg
     7081 cctccgcctt cgccctcgtt gcgggctgga cggtgcatcg tatgctgcat cgccggtcgc
     7141 tgatcctgct gccccgcttc gtcgcgattc caggcgccct gtttatcgtt ttcaccatct
     7201 tctcgctgat gtggggacgg atcacgcttg atccgcgtgt caacttttat ccagcatttc
     7261 tgagagtgca gctagctgcc gctgcattgt cgatcgtttc ggtcggtatc gttttcgtcg
     7321 gtgccgacct cctccgccat cgctcgacgc gaggcgcgtt gatcggcttc tatatcgccg
     7381 ccggattcct tgctctgccg ctccggtcat tctcgctggg cgcgatcggt gaacagcttt
     7441 cgttcatcaa cacacgaggt ctgttcggcc tctggttcgt tgccatctgt tctgcattcg
     7501 gcgttgctgg gggaagtcgt ccgcttgtgg tgcgactggc gctcataggt ggagccgtcg
     7561 gctgggtcat gatggccttc ttccgtgaag gcagttgggt cagcggctgg ctaccggctg
     7621 tgattgccat ggtgatcaca accctgttgg cccgcccaaa gatcggagtt ccgctgctga
     7681 ttgcagcctt gattggtgtc accgcgtatt actccgtcgt ctatgactcg ctggtcaccc
     7741 aacaagaaaa agaaggtagc ctggggggtg agttcggacg gatcgagttg tggcaacgaa
     7801 atctcgaggt catcggaccg cgtgtctggc ttggtaccgg cccagccggc tacgctctct
     7861 actacgttac gctggttccc aacgaagcca tgtccaccca tagcaactac atcgacgtgc
     7921 tcgcgcagaa tggtgcgccc ggcttgttga gcttgcttgg tctgctcggc ggtatcggga
     7981 tcatgggcgt ccagacgttg cggagggtcg ctgatgacga cgatcgtgcc ctctcttgcg
     8041 cagttctggg tggactgccg gcagtgatgg tagcgatctg gtttggagac tggctgatcc
     8101 cgtttgtgta caaccaaacc atcgctggat tcgaccactc ggtctacagc tggctcatgt
     8161 tcgcgacgtt ggctggactt tatgcgcagt accgtacaac cagcgaacaa agcggacctg
     8221 agctccgctt tcgtgtcgtg ctacacccga acgttgctcg agcgctgccg gaataatcgg
     8281 ctcgcggcaa cacttggacg tgcagttgaa ggcagcgtga aagagtggcc atcgggcctc
     8341 ccagatcgtc ttctgaacgg actgtcacgg accctgcgat atctcacacc tcacgacaga
     8401 ttcgagtcct agactcgaag aaacgcgacg gcgaaaggca agacggggtg cgccagcgcg
     8461 cactgttgtt caccgatagt gatgtcgtag atagacggaa atgaaccacc gctgttttca
     8521 cgcacgccta catagttcct gctacatcac gatcttcatc gcagggatgg tgatgttggc
     8581 cctgctgccc gccaatcgtg ttgcggcaag cgagccaatg aactggtccg agccggaaca
     8641 gatcggcacg aacctgtcaa cctcttggtt ccccgagatc ttcgcggacg ccgccggaac
     8701 gattcgtgtt gtttgggacg ccggactcga gcgttctgaa ggaagcctgg ccacgggcag
     8761 atcgggcgtc atgctctccc aatccgtcga ctcgggctgg agcgagcctt ccgagatcct
     8821 gctcggtcaa gcgggatacg tgacgcgccc gatcatcgtc accgattcat catatgcata
     8881 cctgctcttt cgaacgtcgc gcatgtacgt ttcccgggcg ccgctcacat cggatctgca
     8941 aaacgcgcat tcctggtcca ctccatatcc gctgtcgtcc gatgagtcct attggggcca
     9001 gatcgtcgcc cttccggatg gaacggtcgt gaccgtcttc agccagatgg cgagcgtaca
     9061 ggttgctggc aacacggagc gacgtatggc gctcttcatg cgacggtctg ttgaccatgg
     9121 tcagacatgg gggtacgaag agcgcctctc agacacgtcc gaacgaaccg cacgcagttc
     9181 gctcgtcctt gcgccggacg gcaggacgct tctacttgcg tgggacgaag ggtatgacgc
     9241 gcttaccggt ggtggcgatc cgcacgcgat atcgcttctc gcgtctcgtg atggcggcgc
     9301 aacctggggc tcaccgaaca ccatccttgg cgagtacgat cagagcgtgc ttgccaccaa
     9361 tggaacgaca tcgatcctgg tctaccgatc gacaacgaca gatgaactga tgttccgccg
     9421 cagcgaagac ctgggagaga cctggtccga gcaggcgccg attcctggcg tgactgcgag
     9481 accctatacc agtacgcatc actttgacaa actgtccctc gcggtcgatg gcgatggacg
     9541 catcctgctc tcctatatag gccaaccgtc agacaacccg gatcaactct ccgtcatggt
     9601 cgcgacgttc tttgccgccg aatggactac cccgcagatg gtggcgacac cggagggatt
     9661 gcctgagtat ccgcgaatct gcgttggact tggcaatcag gtgcgcctgg tctatttcgt
     9721 tcgtgacagc gaagcgtctg acgagcaacg gcgcacgctt tgggcagtgt ccgcaacgag
     9781 tagtgcacaa ccagccactc ctgtacccta tcaggctgtc tcggtcgaga cgccggttgt
     9841 tacatccgtc ttcgccgcga cccccgttcc acgtccaacg gtcccttctg ctccgtcgcc
     9901 ggtgtctcct ggcaccgttc gaaccgactc cccgcagacc atcgtcatcc aacctgtgct
     9961 gctccttatc gcgagcatct tggccagcgt cgtcgcggtt gcgggagtcg gacgcctctg
    10021 gaccgagata cgcaactgag cgaaaccgta ggcacatctg tcacctaaca gacttgtcaa
    10081 ccggtgctca ttcccattcc accagacgag cgcctccctc tcctacactg acaacggatc
    10141 gacgagacga cgcgcgcttg gaaccgatcc acgagtgtgc caacgcgaat gccggaagca
    10201 actcgacgtg actcgactgg tatgaggcaa ccacgagatg tgtacagaat gctggcagga
    10261 tcgccagctc ttcggtgaat aggcacatgc aaagcgcaga ggcgaccgtc gctcgcaatt
    10321 cgctgttcag cgtcgccggg cagatcgcgc tcaagctgct ctcatttgcg tttgtcatcc
    10381 tggtcgtgcg ccaactgggc gagaccgact atggccgcta ttccgctgca cttgcgtttg
    10441 tcggcatctt ctctgtcttc gccgacatgg gggtcgccag ctacgctgtg cgggagatcg
    10501 ccagatcgcg cgaccgagca ccgtccctgc ttgtcaacat ggcagtcatc agagttgggc
    10561 tggcggtgct cgtcatcgca tgtaccgtcg gtggtgcgtg gctggttggc tacgatcggt
    10621 cgatgatcgg tct
//
LOCUS       DASUGJ010000075         7857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_149086, whole genome shotgun sequence.
ACCESSION   DASUGJ010000075 DASUGJ010000000
VERSION     DASUGJ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149086"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VFV93_01700"
     CDS             <1..1408
                     /locus_tag="VFV93_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5164082.1"
                     /translation="HAMVTLQLKQRADAIDTEVVEQFRTSLRGALIMPGEAGYDAART
                     IWNGMIDKRPGLVVQPAGVADVIQTVNFARENDLLLAIKGGGHNVAGNALCDNGILLD
                     MSSMRAVHVDPRARTAYVEGGAQWGDFDHETAAFKLTSTGGIVSTTGVAGLTLGGGIG
                     WLNARYGASCDNVLAYDIVLADGSYVRASADEHPDLFWALKGGGGNFGVVTGFLFQLH
                     PFDHQVLAGARFYPLDQSREVLEFYRNLTSATSDNVIVYSAIWLNPATGDHASALAFC
                     HSGGLEEAEREMATARVFGNADLDIISPMPYTAWQQAFNPLVPHGRNYYWKALLFSSL
                     SDAALDVIAQQGAIKPNPTSYAVVEQFGGDYGRHAKDETAFWHRDARYQVVIGGAWDD
                     PAEQERCIRWVRGFHDALAPHAATGRNLNFTVVDQDERTPRIRAASGDNYDRLAQIKG
                     KYDPGNMFRVNNNITPAS"
     gene            1746..2315
                     /locus_tag="VFV93_01705"
     CDS             1746..2315
                     /locus_tag="VFV93_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5164083.1"
                     /translation="MTLVGRYHQSLLRVASLYVSSGAAAEDVVQETWLGVLRGLERFE
                     GRSSLKTWIFRILINRAKTRGERERRSIPFSALDSVENDGDEPAVEPERFLPAEHPRW
                     PHHWASFPRNWDELPEHILLSTETRTILQRAIDALPPTQREVITLRDVEGWTSEDVCH
                     ALVLTETNQRVLLHRARSKVRRALEQYLD"
     gene            2332..2589
                     /locus_tag="VFV93_01710"
     CDS             2332..2589
                     /locus_tag="VFV93_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5164084.1"
                     /translation="MTGTLEDMTCQELVELVTEYLEGSLPPLEFQRFEQHLASCAGCA
                     AYVDQMRRTIQLLGQIVVDDIPADEQSDLIELFRNWRQSSP"
     gene            2791..3585
                     /locus_tag="VFV93_01715"
     CDS             2791..3585
                     /locus_tag="VFV93_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5164085.1"
                     /translation="MTLTTAAGTQVQVNGTSLYVEEHGQGDPLVLVQPGLLAGGVYAG
                     VAALLGEQYRVITFDNRGHGRSTNPSSEMSYELFAEDTVALIEALKLERPFVGGWSDG
                     GQVALEIGVRYPDLARALIVGAASLELQSDASHEMMRSFFQVNVDGVVDFDEFAAGHG
                     QMMLPMLRRFHPHGEEHWQSVLQRSATMWLAYAGLTREQVATIETPALVIQGDRDDVI
                     PVDEGVKIYRWLPNAELAILPGADHMRPIFDPTVFAHVVSDFLQRH"
     gene            complement(3716..5257)
                     /locus_tag="VFV93_01720"
     CDS             complement(3716..5257)
                     /locus_tag="VFV93_01720"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX5164086.1"
                     /translation="MTTTRIMRWMAVPIAALLVLVLVSGVAAQLNFGQPVPGQRVYDR
                     TGALSAEQIASLEQRAGGVEAAGSPVFIYLQARDTDYDATVEDARNLMDVWDVQSATD
                     ARDGVVIFFNLQPDDLAHGDYAVVAGKALIDGNLPQRELDRITDRMQPLLEDGDIAGA
                     IGLALDTIARDLREGPPPPPPPSSIEQFSDDIAGSAFSILNAVAVAIAAVAAWLIARA
                     FPARRSSNAPVSPATAPPDMLQPGLAGALVTGSVSDVHISATILDLAARGALAIEPDG
                     KKKVQIRVLDGALVRPGFEEAVWNAFASRADADGVVAGKQLDAARKDWGDVRSMIQSE
                     LVQRGWFDPIAGKQRTPFYIAAVGLFASGIGAVIVAAIAESPWALVAVGVLATVAVLA
                     FVAAVMIPNTTPDGDAVAAPWRGYQRYLKSAGRNPQVNLDLDTAVPYAVALGASGQLD
                     KRLKSASAEGYLPAWLGGSQAEGVWGAGFYPYWIAFNSSVSPTSTMTASTGASAGSGA
                     SGGSF"
     gene            complement(5478..6356)
                     /locus_tag="VFV93_01725"
     CDS             complement(5478..6356)
                     /locus_tag="VFV93_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX5164087.1"
                     /translation="MTTANTLKTAGLLAALTGLFVVIGGYVGGVGGATVFLVLALVMN
                     GVMFWFSDKLALKMAGAREVSEAEAPELHSMIGHLATLARLPKPRVYLIETETPNAFA
                     TGRSPKHSAVAVTTGIQRLLTRDELAGVIAHELAHIRHRDTLISTIVGSVAGAITWIG
                     TMLQWSAIFGGMGRDDENGGNAFGMLAMAILAPIAATIIQLAISRSREFSADAGGAQI
                     LGDPLPLASALEKLQMGNQRRPLAQGNPAMEHLYIVNPFGGRTVSKLFSTHPPTEERI
                     AKLQQMALQPSRAFAR"
     gene            6550..>7857
                     /locus_tag="VFV93_01730"
     CDS             6550..>7857
                     /locus_tag="VFV93_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164088.1"
                     /translation="MRPGVFPVGLAVVVGLAAMLRFWSIDFGLPLVTHPDEPLIVDAA
                     DQMISGRTLNPGWFRYPALIIDIEAALLSVIYAIDTLVGLSTDTIRSLGYGGGRIVMA
                     GFGVATVALTGLLGRKLARLSLVVDRDDRSPSYDWLADIAGLVAAGMLAVSFIHVKDS
                     HYLKPDIPTGFFTTLTLWFALGAWEKPLPQPAPLRREGALDFVRRRAPSLLRGAGWGR
                     GLVTPWLLAGIAVGLAAATKYTGAVVAIVPAMTLVLAWRSDGGMTLRQAAWIAVWMGL
                     AAITVFLLVNPYILLAPREFLSPVDGIRAEFVHYRTGHDGAEGNDSWRWYLAEIWHHG
                     FGPTLTPLIPLGALLAIQRLVADRRAARNGRSGTSALWLVLIFIPLYYAIIARYPVRF
                     DRQLIPILPYLALLGGFGVAATLALLARWPADRRGRQTATMTIC"
BASE COUNT         1356 a         2512 c         2617 g         1372 t
ORIGIN      
        1 gcacgccatg gtcacgctac aactgaagca gcgcgccgat gcgatcgata ccgaggtcgt
       61 cgagcagttc cgcacgagtc tgcgcggcgc gctgatcatg cctggtgagg caggctacga
      121 tgcggctcgc acgatctgga acgggatgat cgacaagcgc ccgggtctcg tcgtccagcc
      181 ggccggtgtg gcggatgtca tccagaccgt caacttcgct cgtgagaacg atctgctgct
      241 ggccatcaag ggcggcggcc acaacgtcgc cggcaatgcg ctctgcgaca acgggatcct
      301 gctcgacatg tcgagcatgc gcgccgtcca tgtcgatccg cgcgcgcgaa cggcctatgt
      361 cgaaggcggg gcgcagtggg gtgacttcga ccacgaaaca gccgccttca agctgacctc
      421 gaccggcggg atcgtctcga cgaccggtgt ggctgggttg acgctcggcg gcggcatcgg
      481 ctggctcaac gcgaggtacg gcgcgtcctg tgacaacgtc ctggcctacg acatcgttct
      541 ggccgatggc agctacgttc gcgccagtgc cgacgagcat cccgacctgt tctgggccct
      601 caaaggcggc ggcggcaact tcggtgtcgt gaccggtttc ctgtttcaat tgcacccgtt
      661 cgaccaccag gtgctggccg gtgcgcgctt ctatccgctc gatcagtcgc gcgaggtgct
      721 cgagttctac cgcaacctca cctcggcgac ctccgataac gtcatcgtct actcggcgat
      781 ctggctcaat ccggcgaccg gcgatcacgc gtccgcgctg gctttctgcc actcgggcgg
      841 tctcgaagag gccgagcgcg agatggcgac ggcgcgggtg tttggcaacg ccgacctcga
      901 catcatctcg ccgatgccgt atacggcctg gcagcaggcg ttcaacccac tcgtgccgca
      961 cggtcgcaac tactactgga aggcactgct cttcagcagc ctgagtgacg cggcgctgga
     1021 tgtcatcgcc cagcagggcg ccatcaagcc gaatccaacc tcgtacgcgg tcgtcgagca
     1081 gttcggcggc gactatggcc gccacgccaa ggacgagaca gccttctggc atcgggatgc
     1141 gcgctatcag gtcgtcatcg gcggcgcctg ggacgatccg gccgagcagg agcgctgcat
     1201 ccggtgggtg cgtggcttcc acgacgctct ggccccacat gccgcgaccg gccgcaatct
     1261 gaacttcacc gtcgttgacc aggacgagcg cacgccgcgc attcgggccg cttccggcga
     1321 caactacgac cggctggcac agatcaaggg caagtacgat cccggcaaca tgttccgggt
     1381 caacaacaac atcacgccgg cgagctagcc gtaggctcgc cgcgcggcca cggcacaggt
     1441 tcgtaacggt ctgagcagcc ggttcgacgc ccccggcacg gccaagggcg tcgaaccggc
     1501 caaacagatg gtcggctgag acgggacgat cggcgtctcg ctgagacatg atccgaagtg
     1561 gaggcatctg gtctgttgca gcgctggtca tcgccgctgt gcctgctatg atcgtggtgc
     1621 gtgcgtcata gtaagagtgc gacgggatga cacaggaatc tccgatgtcc tctatctcgc
     1681 cgattggacc gactgacgat ctggcgctga tcgaggcgct acggcgtggt gacgaagcgg
     1741 cgttcgtgac acttgtgggc cgctatcacc agtcactgct gcgcgtggcg tcgctctacg
     1801 tttccagcgg ggcggcagct gaagatgtgg tccaagagac gtggctcggt gtgttgcgcg
     1861 ggttggagcg tttcgagggc cgctcatcgc tcaagacctg gatcttccgc atcttgatca
     1921 accgggcgaa gacgcgcggc gagcgcgagc gccgcagcat cccgttctcc gcgctcgaca
     1981 gcgtcgaaaa cgacggtgac gagcccgctg tcgagcccga gcgctttctg ccggccgagc
     2041 atccgcgctg gccacatcac tgggcctcct tcccccgcaa ctgggacgaa ctgcccgagc
     2101 atatattgct ttcgacggaa acccgtacca ttttgcagcg cgcgatcgac gcgctacccc
     2161 cgacgcaacg cgaggtcatc accttgcgag acgtcgaggg ctggacgtcg gaggatgtct
     2221 gtcacgcgct cgtgctcacc gagaccaatc aacgtgtgct gctgcaccgt gcccggtcaa
     2281 aggtgagacg ggcactcgag cagtatctgg attaagagtt ggcggtagca attgaccggg
     2341 acactcgaag acatgacctg ccaggaactc gttgaactgg tcactgagta cctcgaaggc
     2401 agcctgccgc ccttggagtt ccagcgcttc gagcaacacc tcgcctcgtg cgccggctgc
     2461 gccgcctacg tcgatcagat gcggcgcacg atccagctgc tcggccagat cgtcgtcgat
     2521 gacattccgg cggacgaaca gtccgacctg atcgagctgt ttcgcaactg gcgccagtcc
     2581 agcccgtaat cccagagccc gcgcttaagc gacgaccgaa cacgatgaca tcagtcaggc
     2641 gcgcgtgcca actcgtcatg cggagatgta acgtctggat cgttccggaa gactatccga
     2701 aagcagcagt tgtcagcatg cgtctcagcg cagtgtggtg cgatgatcga cgctcgctac
     2761 acctcaccca atacgaaagg gatcacagcc atgacactga cgacggcggc gggaacgcag
     2821 gtccaggtga acggcacaag cctgtatgtt gaggagcatg gccagggcga cccgctggtg
     2881 ctggtgcagc cgggcctgct ggcgggtggc gtctacgcag gtgtggcagc actcctcggc
     2941 gagcagtacc gtgtcatcac gtttgataac cgtggacacg gccgctcgac caaccccagc
     3001 agtgagatga gctacgaact gttcgccgag gacaccgtcg cgctgatcga agcgctgaag
     3061 ctcgagcgtc ctttcgtcgg cggttggagc gatggcggtc aggtggcact ggagatcggc
     3121 gtgcgctatc ccgaccttgc ccgagcgctt atcgtcggcg cagccagcct cgaactccag
     3181 agcgatgcct cgcacgagat gatgcggtcc ttcttccagg tgaacgtcga cggtgtcgtt
     3241 gatttcgatg agttcgccgc cggtcacggc cagatgatgc tcccgatgct gcggcggttc
     3301 caccctcacg gcgaggaaca ttggcaatcg gtcctccagc gctcggcaac gatgtggctg
     3361 gcctatgccg gcctgactcg tgagcaggtc gccacaatcg agacaccggc actggtcatt
     3421 cagggcgacc gcgacgacgt cattcccgtt gacgagggcg tgaagatcta ccgctggctg
     3481 ccgaacgccg agctcgcgat cttgccagga gccgaccaca tgcgcccgat cttcgacccg
     3541 accgtcttcg cgcacgtcgt cagcgatttc cttcagaggc actgaacctt cgttccccct
     3601 gtcgtcctga ggccgcagcc gaaggatctc tccgcctcga cgccatgcaa ggctaacgca
     3661 ggggaggtcc ttcgctgcgg ctcaggacga caggggagct gtgtaccctg gaggctcaga
     3721 agctgccgcc ggaggcgccg ctgccggccg aggcgccggt cgaagcggtc atcgtgctgg
     3781 ttggggagac gctgctgttg aaggcgatcc agtaggggta gaaaccggcg ccccagacgc
     3841 cctcggcctg cgagccgccc agccaggccg gcaggtagcc ttcggcactg gccgacttga
     3901 gccgcttgtc gagttgcccg cttgcgccga gcgcgaccgc gtacggcacg gccgtgtcga
     3961 gatcgagatt gacctgcgga ttcctgccgg ccgatttgag gtagcgctgg tagccgcgcc
     4021 acggggcggc gacagcatcc ccatccggcg tcgtgttggg gatcatgacg gccgcgacga
     4081 aggcgagcac agcgacagtc gccagcacgc cgacggcaac cagcgcccag gggctctcgg
     4141 cgatggcagc gacgatgact gcgccaatcc ctgacgcaaa caggccgaca gcggcgatgt
     4201 agaacggcgt tcgctgcttg ccggcgatcg gatcgaacca gccgcgctgg accagttccg
     4261 actggatcat cgagcggacg tcgccccagt ccttacgggc cgcgtcgagc tgcttgccgg
     4321 cgacaacccc gtcggcatcg gcgcgggacg cgaacgcgtt ccagacggct tcctcgaagc
     4381 caggacgaac cagcgcgcca tccagcacac gtatctggac cttcttcttg ccgtccggct
     4441 cgatcgccag cgcgccgcgt gcggccaggt cgaggattgt cgccgagatg tggacatccg
     4501 agacactacc ggtcaccagc gcgccggcca accccggctg gagcatgtcg ggcggggcgg
     4561 tcgccggcga gaccggcgca ttcgagctcc ggcgggccgg gaaggcgcgc gcgatcagcc
     4621 aggcggcgac ggccgcgatc gcgacggcaa ccgcattgag gatgctgaac gcactgccgg
     4681 cgatgtcgtc ggagaactgt tcgatgctgc tcggcggtgg aggaggcggt gggccttcgc
     4741 gcaggtcacg ggcgatcgta tcgagggcca ggccgatcgc cccggcgatg tcgccatcct
     4801 ccagcagcgg ctgcatccga tcggtgatcc ggtccagctc gcgctgcggg agattgccat
     4861 cgatcagcgc cttgccggcg acgacggcgt agtcgccgtg ggcaaggtcg tcgggctgga
     4921 ggttgaagaa gatgacaacg ccgtcacggg cgtccgtcgc cgattggaca tcccagacgt
     4981 ccatcaggtt gcgggcatcc tcaacggtcg catcgtagtc ggtgtcacgc gcctgaaggt
     5041 agatgaagac gggcgaaccg gccgcctcga caccgccagc gcgctgctcc agactcgcga
     5101 tctgctcggc ggacaacgca cccgttcggt cgtagacccg ctggccgggg accggctggc
     5161 cgaagttcaa ttgggcggcc acaccggaga cgagcacgag caccagcagg gctgcgatcg
     5221 gtacagccat ccagcgcatg attcgtgtgg tggtcatgag gctgtgattc atcgtctctt
     5281 ccagcctggg atcggcgtga tagcgctata acgtgtacca gaccgatgtc accgcaggat
     5341 gatcgccagc gccggggcgt tcgaaccgtc gtatcgaaga ccgcccaatg gcccaggcga
     5401 ccggcgttca cgatgaccgg gcaaggggag cggtcctcgt gaacgccggt cgcctgggcc
     5461 attgtgaaat ccgcaggtta gcgggcaaag gcgcggctgg gctggagggc catctgctgg
     5521 agcttggcga tgcgttcctc ggttgggggg tgggtgctga agagcttgga gacggtacgc
     5581 cccccgaacg ggttgacgat gtagagatgc tccatcgcgg ggttgccctg cgccagcggg
     5641 cggcgctggt tgcccatctg cagcttttcg agcgcgctgg ccagcggcag cgggtcgccg
     5701 aggatctgcg cgccgccggc atcggccgag aactcccgcg agcgggagat ggccagttga
     5761 atgatcgtcg ccgcgatcgg cgcgaggatc gccatcgcca gcatcccgaa ggcgttgccg
     5821 ccgttctcat cgtcgcggcc catgccaccg aagatggccg accactgcaa catcgtcccg
     5881 atccaggtga tcgcgccggc gaccgagccg acgatcgtgc tgatgagggt atcgcgatgg
     5941 cggatatggg ccagctcgtg ggcgatgaca ccggccagct cgtctctggt cagcagtcgc
     6001 tgaatgccgg tcgtcacggc gacggctgaa tgcttcgggc tacgaccggt ggcgaaggcg
     6061 ttgggcgtct cggtctcgat caggtagacg cgcggcttgg gcagccgcgc cagcgtcgcg
     6121 aggtggccga tcatgctgtg cagttcggga gcttcggcct cgctgacctc gcgggcgccg
     6181 gccatcttga gcgccagctt gtcagagaac cagaacatga ccccgttcat gacaagcgcc
     6241 agcacgagga agaccgtcgc gccaccaact ccgccaacat agccgccgat cacgacgaat
     6301 agacccgtta gcgctgccag caacccggcc gtcttcaacg tattcgcagt cgtcattatt
     6361 gtgtgctttc caaatagccg gacaggaaca gaccccggtc aatcagaccg gctgcacata
     6421 ttttggggac tcaggatgaa aactacatga aggggtggtg acttcggggt gacgagacgc
     6481 cccagacggt ataactcctc gatgctggcg acggaggggt ggcgcaaagg acacaacacg
     6541 aggtggcggg tgcggccagg ggtgttcccg gtcgggctgg cggttgtcgt tgggctggcg
     6601 gccatgttgc gcttctggtc gatcgacttc gggctgccgc tcgtgacaca ccccgacgag
     6661 ccgctgatcg ttgacgccgc cgaccagatg atctccggaa ggacactcaa tcccggctgg
     6721 ttccgctatc cggccctgat catcgacatc gaagcggcgc tactgtccgt gatctacgcg
     6781 atcgacacgt tggtcgggct gagtaccgac acgatccgct cgctcggcta cggcggcggg
     6841 cggatcgtga tggccggctt cggggtcgcg acggtcgcgc tgaccggcct gctcggccgg
     6901 aagctggctc gtctttcgct cgtcgttgat cgggacgatc ggtcgccgtc gtacgactgg
     6961 ctggcggaca tcgccgggct ggtcgcggcg gggatgctgg ccgtgtcctt catccacgtc
     7021 aaggactcgc actacctcaa accggacatc ccaaccggct tcttcacgac gctgacgctg
     7081 tggttcgcgc tgggggcgtg ggagaaaccc ctcccccaac ccgctcccct aagaagagag
     7141 ggggccttag actttgtgcg tcgtagagcc ccttctcttc ttaggggagc gggttggggg
     7201 aggggtttgg tgaccccttg gcttctcgcg ggcatcgcag tcgggctggc ggcagcgacc
     7261 aagtacacgg gggcggtcgt tgcaatcgtg ccggcaatga cgctcgtgct ggcctggcgc
     7321 agcgacggtg ggatgacact gcgtcaggca gcgtggatcg cggtctggat ggggctggcg
     7381 gcgatcaccg tctttctgct cgtcaatcca tacatcctgc tcgcaccacg cgagttcctc
     7441 tcgcccgtcg acggcattcg ggccgagttc gtgcattacc ggaccggcca cgacggcgcc
     7501 gaggggaacg actcgtggcg ctggtacctg gctgagatct ggcaccacgg gttcgggcca
     7561 acgctgacgc cacttattcc gctcggcgcg ctgctggcga tccagcggct agtcgcggac
     7621 cggcgagcgg ctcgaaacgg acgttcgggg acttcagcgc tctggctggt actgatcttc
     7681 atcccgctct actacgcgat catcgcgcgc tacccggtcc gcttcgaccg gcagttgatt
     7741 ccgatcctgc cgtatctggc gttgctgggc ggtttcggcg tcgcggcgac actggcgctg
     7801 ctcgcgcggt ggccggcaga tcgccgtgga cgacagacgg caacgatgac catctgc
//
LOCUS       DASUGJ010000076         9320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1539288, whole genome shotgun sequence.
ACCESSION   DASUGJ010000076 DASUGJ010000000
VERSION     DASUGJ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9320
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539288"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..494)
                     /locus_tag="VFV93_01735"
     CDS             complement(3..494)
                     /locus_tag="VFV93_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164089.1"
                     /translation="MTDSNDRAKLIPIWEQHRNRILSYGHALHALLPEGEPPITLHGR
                     ELRPADEAVGFQFQFTLAGDLYNGDVRSACFALQVELVRRMTSVGVKVDAAPLLEYGD
                     GHYEVFSRHGTIAPLGDKLALREYFARSLTPAAERFFVRVRAGQPMDEVIAGFAEPHR
                     RRE"
     gene            680..2266
                     /gene="tig"
                     /locus_tag="VFV93_01740"
     CDS             680..2266
                     /gene="tig"
                     /locus_tag="VFV93_01740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX5164090.1"
                     /translation="MRVTVERMPGSVAEVDIFADDAEFSEAFNKAYRKIAREVAIPGF
                     RKGKAPRHIIEGMIGRDAIVEQAGQDMMDDLFRRAIEQENLIPVGEPRVGILQPEPIG
                     FKVVVEVYPEVKLGDYRSVRVEPREVEIEDSEVEEVVDQIQKSRATFEELETARQPMD
                     GDQVILDYTVFEGEEQFQDPVEDAAFILGESGIFDSLSEAIKMMSPGTTAAVTLAFDE
                     DDESVSPELRGKELSYEITLKEVKRRVLPERDDELATTAGDFETYDAMIEQIRKDLLR
                     NKATQTRNEVVTEVINAIAETSEVEIPSTMVEKEIDDELTQFRSRLAQQRVTLEDYLA
                     AENQSMEDLRDEIRPNAERRVRNTMVLQEVAKAEDLKVTDEDIAAEIDRLVVGMQDPD
                     RMRGIYQSEHFRGLLENEIYDRKLTDLVVDIATEGQGAITGAGAEALKAAMEPPAPAE
                     ATESADDDAAVVDVESMAADTAPVDAGDDATEVSAEAIAEAEEVVEAEPAQPTTNEPP
                     AEPVAAADIDAEETEDVQPA"
     gene            2342..3007
                     /locus_tag="VFV93_01745"
     CDS             2342..3007
                     /locus_tag="VFV93_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX5164091.1"
                     /translation="MHDISYPQNLVPMVVESTNRGERAYDIYSRLLRDRIIMLGTPID
                     DQIANLISAQLIFLDYENPEQDIRLYIDSPGGSVYHGLFIYDTIQALRCDVATYACGL
                     TASMGAVLLSAGTHGKRFALPNSRILIHQGSAGFRGNVPDIEIVARETLLLTTRLVEI
                     MALHTGQPFDVIKRDTERDKYLSAEEAKEYGLVDHVLESPRLEALRALRASENGKAAL
                     PEG"
     gene            3111..4379
                     /gene="clpX"
                     /locus_tag="VFV93_01750"
     CDS             3111..4379
                     /gene="clpX"
                     /locus_tag="VFV93_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX5164092.1"
                     /translation="MTGSRGGRVHYRCSFCGKGQEEVRRLIAGPGAVYICDECVQLCR
                     EIIEEEEAPAQRPEPSFTRIPTPRKLYEQLNQYVIGQERAKKILSVAVYNHYKRVAAV
                     TEDDEVELQKSNILLVGPTGCGKTLLAQTLARILDVPFSIADATALTEAGYVGEDVEN
                     ILLRLIQNAGDVARAQTGIIYIDEIDKIARKSDNPSITRDVSGEGVQQALLKIIEGTV
                     ANVPPQSGRKHPHQEYIQIDTRNILFICGGAFEGMEDTIRKRVGKTAAIGFGAQDSSD
                     ETELDNLMSQVNHDDLLRYGLIPEFVGRLPVVVALDHLTHDDLMRILVEPKNAVVKQY
                     QKFFKLDEVDLQFTPDALQAAANQAERRKTGARGLRTTIEEVLLDVMYEIPSLDNVRK
                     CIVNADVINNRTRPLLMTVNDRPIEYEKTA"
     gene            complement(4503..4937)
                     /locus_tag="VFV93_01755"
     CDS             complement(4503..4937)
                     /locus_tag="VFV93_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEX5164093.1"
                     /translation="MNERLEMLQIASKAWLGLKKLAESIPDAALEQPNTIGIWSGRDM
                     LGHLAGWEAIGIDIVNQLNERGEYTPLGVNRETIDAFNEEMLIPYRAMTTREVRQSLE
                     DTHFALMQLAEASQADIADVVLDVTRDHYNKHLGDLRGIPRR"
     gene            5099..6154
                     /locus_tag="VFV93_01760"
     CDS             5099..6154
                     /locus_tag="VFV93_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164094.1"
                     /translation="MAQAHALSADEVGEGQLGWILAHQVRDRAGKRLFRKGMVLDAEA
                     LARWDEAERGELHLIEPAPDDLHEDEAGRRVAAVVAGDGIWAKGPFQSRYHLISDRKG
                     LLRVDRDLLRRINLVTGVTVFTLLDRQPVLPGKVVAGVKVTPLTIPEASVAEVEAIVA
                     GAGEPPMVVLPFAPKRVAVLATEGLNEKLRERFREVVEKKMAWYGSEVTEIRFLSSDR
                     AEIAATMREFLNSGAEVVMAAGGNTIDPLDPLLLALTDVDAEMVHFGGPSHPGSMFWM
                     AQAGDIPVINLATCSMYSRSTVADLILPMVMTGHQVTSADIIEIGYGGLMEREMAFRF
                     PDYDAEWSDEGDKAADE"
     gene            6252..7643
                     /gene="allB"
                     /locus_tag="VFV93_01765"
     CDS             6252..7643
                     /gene="allB"
                     /locus_tag="VFV93_01765"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEX5164095.1"
                     /translation="MAEPRVVIHGGTIVTSDGPIRADLIVRGEEIAGIVADAGDIDAA
                     ERIDATGLVVVPGGVDVHTHFRDPDPSAVEGFETGTRGAIAGGITTAIEMPQAAPTSS
                     TGEHIRIKRGLLEERSHIDIALWGGIVGQPQDQIQEMIDEGIVALKAFMPASSPGFPK
                     SDDQVLLDTFQFLAMSGSDMPFGIHCENDSLLTAGIARMQAAGRKDPLAHAESRPPLV
                     EVEAIHRALFFAELTGARLYVCHVAAADGLALVKQARARGVWVEAETCPQYLSLDHTD
                     LIRHGPFARCAPALRDREEVERIWGYVTDGTVDVVSSDHCGYTIESKLAGMDDIWQAP
                     LGCSGIQTIYPTLFDEIVNKRSLGLARFAELSATNPARIFGLYPKKGVIQVGSDADLA
                     FYDPSSEWLVRGEDMLHRNKWTPFEGKTVGASVVRTMLRGKTVFDRAAQELVVGTPGD
                     GKFLARGYGLGQA"
     gene            7916..9244
                     /locus_tag="VFV93_01770"
     CDS             7916..9244
                     /locus_tag="VFV93_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX5164096.1"
                     /translation="MPPITTNSPFVAPPRISQATFERVLAEAGSPWAARGGEIYAVIV
                     AAGHDPAVWLAICAEEHSYGTNRNSVLWRNDTRSWTNARTVRDPGISGWAIVHDAVRD
                     SDYVRYADVLDSVRDGCYRISDPTYRYVREGRTSIAAVFAIWTEGDGARYAAAVVERV
                     NGWMLSEEPAPYASVISGLIDIRGRLARRDRRDGVPAGPYETRPMAQKRGVVVHYSGP
                     PVGQRDNPLAVLQSEARYHIGKDWSHPGDPPVRGDGLMYHVAIGDDGTAYLCRDLEAV
                     LWHCGAWPQNAIALSLHVPIGGSQRATAAQLATLRRVVDEWRQATGTPINEVWGHQEL
                     QPTDCPGTLMADFVYPYRAGEGGTPMADGKWFPETGCYVGGAFWTYWRERGGLPIFGY
                     PLTNERDEGGMTVQYFERAVFEWHPANNDPYKVLLRRLGADALARLEVAS"
BASE COUNT         1729 a         2925 c         3134 g         1532 t
ORIGIN      
        1 ttctattcac gtcgccggtg cggttccgca aacccggcga tcacttcgtc cattggttga
       61 ccggcacgga ctcgaacaaa gaagcgctcg gccgccggtg tcagtgatcg cgcgaaatac
      121 tcgcgtagcg cgagcttgtc accgagtggc gcgatcgttc cgtgccggct aaacacctca
      181 tagtgcccat caccgtactc cagcaacggt gcggcgtcga ccttgacgcc gacgctggtc
      241 atgcgccgga ccagttcgac ctggagggcg aagcaggccg atctgacgtc gccattgtat
      301 aggtcaccgg ccagcgtgaa ctggaactga aacccgaccg cctcatcggc cggacgcagt
      361 tcgcggccat gcagggtgat cggtggttcg ccctccggca ggagcgcgtg gagggcgtgt
      421 ccgtagctga ggatacggtt acggtgctgt tcccagattg gaatgagttt cgcgcggtcg
      481 ttgctgtcag tcatgcgggg atgacctgcc cgaatggggg attgccggcc ggacgaattc
      541 ggggatagaa ctcccgaggt gagattgtag cagccggggc ggtccgaacc ggcatcaatg
      601 cgccggaaac ggcggctgaa tcggcccgga tgctatactc cgggcgttga aatcgttgaa
      661 acgtattgag gtagccgctg tgcgagtcac cgtcgaacga atgcccggga gtgttgcgga
      721 agtcgatatc ttcgccgatg acgccgagtt ctcggaggcg ttcaacaagg catatcgcaa
      781 gatcgcacga gaagtcgcga ttcccgggtt ccgcaagggc aaggcgcccc gtcacatcat
      841 cgaaggcatg atcggccgcg atgcgattgt cgaacaggcc ggccaggaca tgatggacga
      901 cttgttccgc cgcgcgatcg agcaggagaa cctgattccg gtcggcgagc cgcgcgtcgg
      961 catcctccag ccggagccga tcggcttcaa ggtcgtcgtc gaggtctacc ccgaggtgaa
     1021 gctcggcgac taccgctcgg tgcgggtcga gccgcgcgag gtcgagatcg aggactccga
     1081 agtcgaagag gtcgtcgatc agatccagaa gtcgcgggca accttcgagg agcttgagac
     1141 agcccgccag ccgatggacg gcgaccaggt cattctcgac tacacggtct tcgagggcga
     1201 ggagcaattc caggacccgg tcgaggacgc ggccttcatt cttggtgaga gcggcatctt
     1261 cgattcgctc tccgaagcga tcaagatgat gtcgcccggc acgacggcgg cggtgacgct
     1321 ggcgttcgac gaggacgacg agtcggtctc gccggagctg cgcggcaagg aactgtcgta
     1381 cgagatcacg ctcaaggaag tcaagcggcg ggtcctgccg gagcgtgacg acgagctggc
     1441 aacaaccgcc ggcgacttcg agacctacga cgcgatgatc gagcagatcc gcaaggacct
     1501 gctgcgcaac aaggcaaccc agacccgcaa cgaggtcgtg accgaggtga tcaacgcgat
     1561 cgccgagacc tccgaagtcg agatcccgtc gacgatggtc gagaaggaga tcgacgacga
     1621 gctgacccag ttccgttcgc ggctggccca gcagcgtgtg acgctggagg actaccttgc
     1681 cgctgagaac cagtcgatgg aggacctgcg cgacgagatc cgcccgaacg ccgagcgtcg
     1741 ggtccgcaac acgatggtgt tgcaggaggt cgccaaggcc gaggacctga aggtcaccga
     1801 cgaggacatc gcggccgaga tcgaccggct ggtggtcggt atgcaggatc ccgaccggat
     1861 gcgcggcatc tatcagtcgg aacatttccg tggactgctt gagaacgaga tctacgatcg
     1921 taagctgacc gatctggtcg tcgacattgc gaccgaaggt cagggagcga tcaccggcgc
     1981 aggcgccgag gcgctcaagg cggcgatgga gccgccggca ccggccgagg cgacggaatc
     2041 cgctgatgac gatgccgccg ttgtcgatgt ggagtcgatg gccgccgaca cagcgccggt
     2101 cgacgccggc gatgatgcca ccgaagtgag cgccgaggcg attgccgagg ccgaagaggt
     2161 agtggaagct gaaccggccc aaccgacgac caacgagccg ccggccgaac cggtcgcggc
     2221 tgccgacatc gacgccgaag agaccgaaga cgttcaaccg gcctgaggcc ggatagcgag
     2281 gccgctacaa cgcaactgac cgcgccgcgc gacgcgtgag cgccggaaag gaccatactt
     2341 gatgcacgat atctcgtacc cgcagaacct ggttccgatg gtggtcgaga gcaccaaccg
     2401 cggcgagcgt gcctacgata tctactcgcg gctgctgcgc gaccggatca tcatgctggg
     2461 gacgccgatc gacgatcaga tcgcgaacct gatcagtgcc caactgatct tcctcgatta
     2521 tgagaacccc gagcaagata ttcgcctgta catcgatagt ccgggcggaa gcgtctacca
     2581 cggactgttc atctacgata cgattcaggc acttcgctgc gatgtcgcga cctacgcctg
     2641 tggcctgacg gccagcatgg gcgcggtgtt gttgtcggcc gggacgcatg gcaagcgctt
     2701 cgcgctgccg aactcgcgga tcctgatcca ccaggggtcg gccggattcc gcggcaacgt
     2761 gccggatatc gagatcgtcg cgcgcgagac gctgttgctg acgacgcggc tggtcgagat
     2821 catggcactg cacacggggc agccgttcga tgtcatcaag cgtgacaccg agcgcgacaa
     2881 gtacctgtcg gcggaggagg cgaaggagta cggtctggtt gaccatgtgc tcgaatcgcc
     2941 acggcttgag gcgttgcgcg cgctgcgagc ctccgaaaac ggcaaggcgg cgctgcccga
     3001 ggggtaggcg ccacggaccg ggacgaactg gaaaccgagg cagtgacttc gaacgaggac
     3061 gaacgaggac acatagcacg ggccgccggc gctcgtgagg ggatgagacg atgacaggtt
     3121 cacgaggcgg gcgagtccac taccgatgct ccttctgcgg caagggccag gaggaagtcc
     3181 gtcgtctgat cgcgggcccc ggtgccgtct acatctgtga cgagtgcgtc cagctctgtc
     3241 gcgagatcat tgaggaagag gaggctcctg cccagcggcc ggagccgtcc tttacccgga
     3301 ttccgacgcc tcgcaagctc tacgaacagc tcaaccagta cgtcatcggc caggagcgcg
     3361 ccaagaagat cctgtcggtc gctgtctaca accactacaa gcgggtagcc gccgtgaccg
     3421 aggacgacga ggtcgagctc cagaaatcca acatcctgct ggtcggcccg accggctgtg
     3481 gcaagacgct gctggcccag acgctggccc ggattctcga tgtgccgttc tccatcgccg
     3541 atgcgacggc gctgaccgag gccggctacg tcggcgagga tgtggagaac attctgctgc
     3601 gcctgatcca gaacgccggc gatgtcgctc gggcgcagac cggcatcatc tacattgacg
     3661 agatcgacaa gatcgcgcgc aagtcggaca atccctcgat cacccgcgac gtctccggcg
     3721 agggcgtcca gcaggcgttg ctgaagatca tcgagggcac tgtcgccaac gtcccgccgc
     3781 aatcgggccg caagcacccc catcaggaat acatccagat cgacacgcgc aacatcctct
     3841 tcatctgcgg tggcgcattc gaggggatgg aagacacgat ccgcaagcgc gtcggcaaga
     3901 cggccgcgat cggcttcggc gcgcaggatt cctctgacga gaccgagctg gacaacctga
     3961 tgagccaggt caaccacgac gatctactca gatacgggct gatcccggag ttcgtcggcc
     4021 ggctccccgt cgtcgtcgcg ctcgaccacc tgacccacga cgatctgatg cgaattctgg
     4081 tcgagccgaa gaacgcggtt gtcaagcagt atcagaagtt cttcaagctc gacgaggtcg
     4141 atctgcagtt cacgccggac gcgctgcaag ccgcggccaa ccaggccgag cggcgtaaga
     4201 ccggtgcacg cggcctgcga acgacgatcg aagaggtcct gctggacgtc atgtacgaga
     4261 tcccgtcgct cgacaacgtc cgcaaatgca tcgtcaacgc cgacgtcatc aacaaccgca
     4321 cccggccgct gctgatgacg gtcaacgacc gcccgatcga gtacgagaag acggcctagc
     4381 aggtaacaat caaacgacac caaccggtag gggcgaggcc ttgactcgcc ctttttgatg
     4441 cccgcgcggt cagacgcaag cgacggcagc ggccatcgcc ccacgcgtgg ttgacgacgc
     4501 ggtcagcggc gcgggatgcc gcgcaggtcg ccgaggtgct tgttgtagtg gtcgcgggtg
     4561 acgtcgagga cgacgtcggc gatatcggcc tgagatgctt cggcgagctg catcagcgcg
     4621 aagtgggtgt cttcgagcga ctggcggacc tcgcgggtgg tcatcgcgcg ataggggatc
     4681 agcatctctt cgttgaaggc gtcgatcgtc tcgcggttga cgcccagcgg tgtgtattca
     4741 ccccgctcgt tgagctggtt gacgatgtcg atcccgatcg cctcccagcc ggccagatgg
     4801 ccgagcatgt cgcgcccgct ccagatgccg atcgtgttgg gctgctcaag cgctgcgtcg
     4861 gggattgact cggccagctt cttcaatccc agccacgcct tcgaggcaat ttgcagcatc
     4921 tcgagtcgtt cgttcatcgt catcggcatg gctggtctgg accctttcgc gctcggttcc
     4981 tctcgaaacc cgtggctgcc atcacgcagg actatacggc gtggtgatag cgatcggtcg
     5041 cgttcatcgt cattggcggt aggatgaagg gcaatagaac aggcgacggg ggagtggtat
     5101 ggcacaggca cacgcgctat cggcggacga ggttggtgaa gggcagcttg gctggattct
     5161 ggcacatcag gtccgtgacc gggccggtaa gcggctgttc cgcaagggga tggtacttga
     5221 tgccgaggca ttggcccgct gggacgaggc cgagcgcggg gagctgcacc tgattgagcc
     5281 tgcccctgac gatctgcacg aggacgaggc cgggcgacgg gttgcggcgg ttgtggcggg
     5341 cgacgggatc tgggccaagg gtccgtttca gagccgctat catctgatct cagatcggaa
     5401 ggggctgctg cgcgtcgatc gcgatctgct gcggcgaatc aacctcgtca cgggcgtgac
     5461 ggtcttcacg ctgcttgacc ggcagccggt gctgccgggc aaggtggtcg ccggcgtcaa
     5521 ggtcacaccg ctgacgattc cggaggccag cgtcgctgag gtcgaggcga tcgtcgccgg
     5581 ggcaggtgag ccgccgatgg tcgtcttgcc gttcgcgccg aagcgggtcg ctgtcctggc
     5641 gaccgagggg ttgaacgaga agctacgcga acgctttcgt gaggtcgttg agaagaagat
     5701 ggcctggtac ggctcagagg taaccgagat tcggttcctc tcgtccgacc gggccgagat
     5761 tgccgcaacg atgcgcgagt tcctcaactc cggtgccgag gtcgtgatgg cggctggcgg
     5821 caacacgatc gatccgctcg atccgctgct gctggcgctg accgacgtcg atgcggagat
     5881 ggtccatttc ggcggaccgt cgcaccccgg cagcatgttc tggatggcgc aggcgggcga
     5941 tatcccggtc atcaacttgg cgacctgctc gatgtattca cgctcgaccg tcgccgacct
     6001 gatcttgccg atggtgatga ccggccacca ggtaaccagc gccgacatca tcgagatcgg
     6061 ctacggtggg ctgatggagc gcgagatggc cttccgattc cccgactacg atgccgaatg
     6121 gagcgacgag ggcgacaagg cggccgacga atagccaacg gacagacatc cgggtgcgtg
     6181 ctacgatggc gcgcgtcgca atcattggcc gggtcgctga ttcgcccggg ctggggaagg
     6241 ggtgagcatt catggcagaa ccgcgtgtcg tcattcacgg cgggacgatt gtcacatcgg
     6301 atgggccgat ccgggcagat ttgatcgtgc gaggcgaaga gatcgcgggg atcgtcgccg
     6361 acgccggcga catcgacgcg gccgagcgga tcgacgcgac cgggctggtt gtcgtgccgg
     6421 gcggggtcga cgtccacact cacttccgcg atcccgaccc gagcgcggtc gagggattcg
     6481 agaccggcac gcgtggcgcg attgccggcg ggatcacgac cgcgatcgag atgccgcagg
     6541 cggcgccaac ctccagcacc ggcgagcaca tcaggatcaa gcgcgggctg ctggaagagc
     6601 gctcgcatat cgacatcgcg ctctggggcg gtatcgtcgg ccagccacag gatcagattc
     6661 aggagatgat cgatgagggc atcgtcgccc tcaaggcgtt catgccggcc tccagtccag
     6721 gcttcccaaa gtcggacgac caggtcttgc tggacacgtt ccagttcctg gcgatgtccg
     6781 gctccgacat gccgttcggc atccactgcg agaacgactc gctgctgacg gccgggatcg
     6841 cgcggatgca ggcggccggg cgcaaggacc cgctggcgca tgccgagagc cggccgccgc
     6901 tggtcgaggt cgaggcgatt catcgggcgc tcttcttcgc cgagttaacc ggcgcgcgac
     6961 tgtatgtgtg ccacgtcgcg gcagccgatg ggctggccct ggtcaagcag gcgcgagcgc
     7021 ggggcgtctg ggtcgaggcc gaaacctgcc cgcagtacct gtcactcgac cacaccgacc
     7081 tgatccggca tggaccgttc gcccgttgcg cgccggccct gcgcgaccgc gaggaggtcg
     7141 agcggatctg gggctacgtc accgacggga cggtcgatgt cgtctcgtcc gaccactgcg
     7201 gctacacgat cgagtcgaag ctggccggga tggacgacat ctggcaggcg ccgctgggct
     7261 gctccggcat tcagacgatc tacccgacac tgttcgacga gatcgtcaac aagcgctcgc
     7321 tggggttggc gcgctttgcg gagctgtcgg cgacgaaccc ggcccgcatc tttggtctct
     7381 acccgaagaa gggcgtcatt caggtcgggt cggacgcaga cctggcgttt tacgacccaa
     7441 gcagcgagtg gctcgtgcgc ggcgaggaca tgctgcaccg caacaagtgg accccgttcg
     7501 aaggcaagac ggtcggcgcc agcgtcgtcc ggacgatgct gcgcgggaag accgtcttcg
     7561 accgagcagc gcaggaactg gtcgtcggca cacccggcga cggcaagttc ctcgcgcgcg
     7621 ggtacgggtt ggggcaggcg tagatacggc gacagcggag tcatccagtc gtcgatcttt
     7681 ccgcgaagac aacgctcctt gccctgtcac cctgagccgc agcgaaggat ctccaggcgt
     7741 aggagagacg agcgatctgg tagcgacgac gcgttctgcc aatcggcgcg ctcctgtcag
     7801 tcacgttaag cgatccttcg ctgcggctca ggatgacagg gggagcgtat acggtcctcg
     7861 tgaacaacgt tcggctggat cactcagcac tcagcactca gaacaggaga cacccatgcc
     7921 ccctatcacc accaactccc ccttcgtcgc cccgccgcgc atcagccagg cgacgtttga
     7981 gcgcgtcctg gcggaagccg gttcgccgtg ggcggcgcgg ggcggcgaga tctacgccgt
     8041 catcgtcgcg gccgggcacg atccggctgt ctggctggcg atctgcgccg aggagcacag
     8101 ctacggcacc aatcgcaact cggtcctctg gcgcaacgac acccgaagct ggaccaacgc
     8161 tcgaaccgtc cgtgatccgg gcatcagcgg ctgggcgatc gtccacgacg ccgttcgcga
     8221 ttccgactac gtccgttacg ccgacgtgct cgactcggtt cgcgacggct gctaccggat
     8281 cagcgacccg acctaccgct acgtccgcga gggtcggacc tcgatcgccg ccgtcttcgc
     8341 gatctggacc gaaggcgatg gcgctcgcta cgcggccgcc gtcgtcgagc gggtcaacgg
     8401 ctggatgctg tcggaggaac cagcaccgta cgcgtccgtc atcagcggcc tgatcgacat
     8461 tcgcgggcgg ctggcccggc gcgaccggcg cgatggcgtc ccggccgggc cgtacgagac
     8521 gcggccgatg gcgcagaagc gcggggtcgt cgtccactac tccggcccgc cggtggggca
     8581 gcgcgacaac ccgctggctg tcctgcagtc cgaggcgcgc taccacatcg gcaaggactg
     8641 gtcgcatccc ggcgacccgc ccgtccgcgg cgacggcctg atgtatcacg tcgcaatcgg
     8701 cgacgacggc acagcctacc tctgtcgcga tctggaggcc gtcctctggc actgcggcgc
     8761 ctggccgcag aacgcgatcg ccctctcgct gcacgtcccg atcggcggca gccagcgggc
     8821 gacggccgcc cagctggcca cgctgcgccg cgtcgtcgac gagtggcggc aggcgaccgg
     8881 tacccccatc aacgaggtct ggggccatca ggaactgcaa cccaccgatt gccccggcac
     8941 gctgatggcc gactttgtct atccataccg tgcaggagaa ggaggcacac caatggcgga
     9001 tggcaaatgg ttcccagaaa ccggctgcta cgtcggcggc gcgttctgga cctactggcg
     9061 cgagcggggc gggctgccga tcttcggcta cccactcacc aacgagcgcg acgagggcgg
     9121 catgaccgtc cagtacttcg agcgggccgt cttcgagtgg cacccggcca acaacgaccc
     9181 ctacaaggtc ctgctgcgcc ggctcggcgc cgacgcgctc gcccggctgg aggtggcgtc
     9241 atgaccggcc tcgacctggt cgtctacagc ttcgcgctgc cgctgacgat cgccatcgtc
     9301 cagatcctca aggacgccgc
//
LOCUS       DASUGJ010000077         4323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_983148, whole genome shotgun sequence.
ACCESSION   DASUGJ010000077 DASUGJ010000000
VERSION     DASUGJ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983148"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFV93_01775"
     CDS             <1..804
                     /locus_tag="VFV93_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5164097.1"
                     /translation="VTISDYPTLDFTFYGLNLDTTKTTLFQDREVRQALLYALDREAM
                     IESIRFGYGEVAVGTMPVLSWAYNPDGIELKYPYDPDKAKQLLDDAGWVEGSDGIREK
                     DGQRLAFTMYTNAGNQVREQYLTVLQEYWSAIGVEMTPQLEPFPALVERITETFDFEA
                     YLIGFSWGVAPDQTTMWACDSAHGNGFNTVAYCNEEVDKLLQEGLVETDREKRIEIYT
                     QMQNIIMEDLPHVILDFPQGFTGVSNRVHNVFPSDVNVRFNMQQWWVDQ"
     gene            970..1938
                     /locus_tag="VFV93_01780"
     CDS             970..1938
                     /locus_tag="VFV93_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164098.1"
                     /translation="MGRFILRRLLQVIPLLIGISIITFALANLVPGSPVSGLEFNPRT
                     TPEDIARIKENLGLNDPVWKRYFIWIGNVAQGDLGISLKNFRPVRETIMDKLPNTLLL
                     TGTALLLALAVAIPIGVYGAVRRNSAFDNITTATSVAGFSVPTFWLGLMLILLFAVKF
                     KEWGLPSFPAGGSYDLRGGGGFFDRLEHLILPAFALAFVQMAAWTRYIRSQMLEVLNQ
                     DYMRTANAKGLRERIVIFRHGLRNAILPLVTLLGIAIPDLFAGSLIIENIFTYPGIGQ
                     LAFNAAINKDYPLIMGITLVAGTLVILGNLIADVVYSLLDPRIRLD"
     gene            2010..2954
                     /locus_tag="VFV93_01785"
     CDS             2010..2954
                     /locus_tag="VFV93_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164099.1"
                     /translation="MSTVQETERSTTAGARPTVDRNTLERIGKPRTYLGMAWRRLRRN
                     KLAMIGAGIMILMILIALGAGLISEFITHKSLSDQSLRDNYMGIGEGGYILGSDDLGR
                     DTLTRLVYGARVSLGVAGLSVLVALTVGTVVGTLAGYYGKWVDSVLMRFVDIMLSIPT
                     LFLLLLVATLWRMGPWKLALVIAAVAWVTLSRLVRGEVMSVKGRDYIDAARVVGVGNR
                     RIMFRHILPNVAPVIIVWASLVVPGLILTEATLSFLGLGVQPPTPSWGNMLTSAQKVW
                     SHSTAMVLLPGFAIYITVFAINLFGNGLRDALDPRLTD"
     gene            3057..3866
                     /locus_tag="VFV93_01790"
     CDS             3057..3866
                     /locus_tag="VFV93_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5164100.1"
                     /translation="MPSIQVNGVDLSYEVTGDGPPVVLTHGSWGDRRGWAAVVPGLAE
                     SFRVVTWDRRGHGESGDLPGRGTRTQDAADLAGLIEGLGIGPVHAVGNSFGGSITLEL
                     ASRRPELLRSIAVHEPPVFGVLAGSEDGGLSVYRERIGPVVERLAAGEMEAGAALFVD
                     SVVGQPGNWGRMSPEAQAAMTRLAPTFLDENLDPGMTELDLDPLKAFQQPTLLSYGDT
                     SPGYFAAVVLRIARVLPDGRVLVFAGAGHVPHRSHPDAYVDALTGFMQALA"
     gene            complement(3871..4071)
                     /locus_tag="VFV93_01795"
     CDS             complement(3871..4071)
                     /locus_tag="VFV93_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164101.1"
                     /translation="MTTSSTTMPRVVTYTELEQQGFSAEQINRLDQLRACYPYMEFTD
                     SVEEWRRLNFLKWRHSTGRVAE"
BASE COUNT          739 a         1329 c         1394 g          861 t
ORIGIN      
        1 gtgacgatca gcgactaccc gacgctcgac ttcaccttct acggcctgaa cctggacacg
       61 accaagacga cgctcttcca ggaccgtgag gttcgccagg cgctcctcta cgcactcgac
      121 cgcgaggcga tgatcgagtc gatccgcttc ggctatggcg aggtcgccgt cggtacgatg
      181 cctgtcctct cgtgggccta caatccggat gggatcgagc tgaagtatcc gtacgatccg
      241 gacaaggcca agcagctgct tgacgatgcc ggctgggtcg agggctccga tggcatccgc
      301 gagaaggatg gccagcggct ggccttcacg atgtacacca acgcaggcaa ccaggtccgc
      361 gaacagtacc tgaccgtcct gcaggagtac tggtctgcaa tcggtgtcga aatgacgccg
      421 cagctcgagc cgttcccggc gctggtcgag cgcatcaccg agacgttcga cttcgaggcc
      481 tacctgatcg gcttcagctg gggtgtcgcg ccggaccaga ccacgatgtg ggcctgcgat
      541 tcggcacacg gcaacggctt caatacggtc gcttactgca acgaggaagt tgacaagctg
      601 ctccaagaag gtctcgtcga gaccgaccgc gagaagcgca tcgagatcta cacgcagatg
      661 cagaacatca tcatggagga tctgcctcac gtgattctcg acttcccgca gggcttcacc
      721 ggcgtctcga accgggtcca caacgtgttc ccgagtgacg tgaacgtccg gttcaacatg
      781 cagcagtggt gggtcgacca gtagacgtca tgacgggggt gggaaccttc ccacccccgt
      841 tgcccgtccc gatgacgatg caataccatg cgctgtatca gtgacttgct ctgctgaaat
      901 attcaggtag catgttggcg ggaagattca cgtcagcgta cctgtcagga caatcgaaag
      961 gccatatcca tgggtcgctt cattctgcga cggctgcttc aggttattcc gctcctgatc
     1021 ggcatctcga tcatcacctt cgcccttgcc aacctggttc ccggcagtcc cgtgagcggc
     1081 ctggaattca acccgcgcac gacgccggaa gatatcgcgc gcatcaagga aaacctcggt
     1141 cttaacgatc cggtctggaa gcgctacttc atctggatcg gcaacgtcgc gcaaggcgac
     1201 ctcggtatct cgctcaagaa cttccgcccg gtccgtgaga caatcatgga caagctgccg
     1261 aatacgttgc tgctgaccgg cacggcgctg ctgctggcac tggcggtcgc gatcccaatc
     1321 ggcgtctatg gcgcagtccg tcgtaattcc gccttcgaca acatcacgac ggccacttcg
     1381 gtcgcaggtt tctcggtgcc aaccttctgg ctcggcctga tgctgatcct gctgtttgcc
     1441 gtgaaattta aagaatgggg cttaccgtcg ttcccggcag gcgggtcgta tgacctgcgc
     1501 ggcggcggcg gctttttcga ccggcttgaa catctgatcc tgcccgcctt cgcgcttgca
     1561 ttcgtgcaga tggcggcctg gacacgctat atccgttccc agatgctcga agtgctcaac
     1621 caggattata tgcggacggc caacgcgaag gggttgcgcg agcggatcgt gatcttccgg
     1681 cacggcctgc gcaacgccat tctgccgctg gtgacgctgc tcggtatcgc aattccggac
     1741 ctgttcgccg ggtcgctgat catcgagaat atcttcacct accctggcat cggccagttg
     1801 gccttcaacg cggcgatcaa caaggactac ccgctgatta tgggaatcac gctggtcgcc
     1861 gggacgttgg tcattctggg caatctgatc gccgacgtcg tctacagcct gctcgatccg
     1921 cgcatccgtc tggactgacc tggctcgatc gacagtccag tgcatgtgcc atcgcgatac
     1981 agagaaagcc ggcaggaaag gaagcaagga tgagtacggt acaggagacg gaacgctcaa
     2041 cgacagcggg cgccaggcca accgtcgatc gcaacacgct ggagcgtatc ggcaagccac
     2101 gcacctatct gggaatggcc tggcgccggt tgcggcgcaa caagctggcg atgatcggcg
     2161 ccgggatcat gatcctgatg attctgatcg cgctcggggc cggactgatc tccgaattca
     2221 tcacccacaa gtcgctctcg gaccagtcgc tacgcgacaa ctacatgggc atcggcgaag
     2281 gcggctacat cctcggctcc gatgacctcg gccgcgatac gctgacacgc ctcgtctatg
     2341 gcgcccgcgt cagcctcggt gttgccgggc tgtcggttct cgtggcgctg acggttggga
     2401 cggtcgttgg cactcttgcc ggctactacg gcaagtgggt cgacagcgtc ctgatgcgct
     2461 tcgtcgacat catgctctcg atcccgacgc tcttcctgct gctgctggtt gcgacgctct
     2521 ggcggatggg gccgtggaag ctggcgctcg tgattgccgc ggtcgcctgg gtgactctct
     2581 cgcggctggt gcgcggcgag gtgatgtcgg tcaagggccg cgactacatc gatgccgcgc
     2641 gggttgtcgg cgtcggcaat cgccggatca tgttccggca tattctgccg aacgtcgcgc
     2701 cggtcatcat cgtctgggcc tcgctggtcg tgcctggcct gatcctgacc gaggcgacgt
     2761 tgtcgttcct cggcctcggc gttcagccgc cgacacctag ttggggcaac atgctgacgt
     2821 cggcccagaa ggtctggagc cactcgacgg caatggtcct gctaccgggg ttcgcgattt
     2881 acatcacggt cttcgccatc aacctgttcg gcaacggcct gcgcgacgcc ctcgacccac
     2941 gcctgaccga ctaaccggcg ggcgatcagc aactggactg gtactctggg agggcagcga
     3001 tgccctcccg cttgttgctg tcgtgacgcg tgccgcagta cacgaaaggt ggcccgatgc
     3061 catcgatcca ggtcaacggc gtcgatctgt cgtacgaggt gacaggcgac gggccgccgg
     3121 tcgtgttgac gcatggttcg tggggcgacc ggcgcggctg ggcagccgtc gtgccggggc
     3181 tggcggagtc gtttcgggtc gtgacctggg atcggcgcgg gcatggggag agcggcgatc
     3241 tgccggggcg ggggacgcgc acgcaggatg cggctgatct ggccgggctg atcgaagggt
     3301 tgggaatcgg gcccgttcac gcggttggga actcgtttgg tggctcaatc acgctggagc
     3361 tggcgtcgcg ccggccggaa ctgttgcgca gtatcgccgt ccacgaaccg ccggtgttcg
     3421 gcgtcctggc cggcagtgaa gacggggggc tgtcggtcta ccgggagcgg atcgggccgg
     3481 tcgtcgaacg gctggccgcc ggcgagatgg aggccggcgc ggcgctgttc gtcgacagtg
     3541 ttgtgggaca gccggggaac tggggacgga tgtcgccgga agcacaggcg gcgatgacgc
     3601 gcctcgcgcc gacgtttctg gacgagaatc tcgaccctgg catgacggaa cttgatctcg
     3661 atccgttgaa ggcgttccag cagccgacac tactctcata cggcgacacg agtccgggct
     3721 atttcgcggc ggttgtcctg cggatcgcca gggtgctgcc ggatggccgg gtgcttgtct
     3781 tcgccggggc cggccatgtg ccgcatcggt cgcacccgga cgcatatgtc gacgcgttga
     3841 ctggattcat gcaggcgctg gcttgacgga ctactcggcg acgcggccgg tcgagtgtcg
     3901 ccacttgagg aagttgagcc ggcgccactc ctcgaccgaa tcggtgaact ccatgtatgg
     3961 gtaacaggcg cgaagctggt cgagccggtt gatctgctcg gcgctgaagc cttgctgctc
     4021 cagctcggtg tatgtcacga cccgtggcat cgttgtcgat gaagtcgtca ttttgccctc
     4081 gtttcgccag tggcggtccg gatctctggt ggcggccacc gtgctcgccg atccaaccgt
     4141 tgcacccagc atatggaaac cggccccgct gcacatcccc gcaacgatgg gttttcggac
     4201 tgtccaaaag tacgaacggc gctcggtctt tggtaccagc ggggccgggt gggtgcacgt
     4261 tttgtggctg gcaaggttcg cttgcctccc cgcgtagggg cgatggccgc agcctcgccc
     4321 ttc
//
LOCUS       DASUGJ010000078         4032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1677476, whole genome shotgun sequence.
ACCESSION   DASUGJ010000078 DASUGJ010000000
VERSION     DASUGJ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677476"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..698)
                     /locus_tag="VFV93_01800"
     CDS             complement(15..698)
                     /locus_tag="VFV93_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5164102.1"
                     /translation="MAFSGYRVKAMVDEQTVEINGIRVHYARIGAGPALVLVHAGGTQ
                     SSWHTWRDNLAALSRHYTVLIPDLPGLGESGPPPAPIDSEDDFFRYYPRFIEAFIRAT
                     APEGAVMIGAASGGGICLTVAARYPEIARALVLVDAEVIGRVIETETALLRCPTLIVW
                     QHDDMLVPPEHATTLAGRIPGAELRLFPGDHTRDEWGGNMPHRLWPSEFDATVLDFLN
                     RVTTQRQAG"
     gene            815..1474
                     /locus_tag="VFV93_01805"
     CDS             815..1474
                     /locus_tag="VFV93_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164103.1"
                     /translation="MPDGSEFRNQVLERYDFTPEAQAILRQIPVEIHDLTHTNGGGGW
                     YPGERRIILNGVQDEACLHELAHAWADETGFYVDPHPDDPDRNGRNFAYRRDVDRAAI
                     ESDPAFQRVAFLAWEYTNGNPRTGFEGMKEIDWERFAGLASGVMGDIRLMPPYLRRWY
                     EPLFGGHPHVSGPSELPSTVPVNWRQGIGLVGTMWPAAAMPGGGVLTLLDRLRRWLTR
                     R"
     gene            complement(1476..>4032)
                     /gene="cphA"
                     /locus_tag="VFV93_01810"
     CDS             complement(1476..>4032)
                     /gene="cphA"
                     /locus_tag="VFV93_01810"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5164104.1"
                     /translation="NTIEGFNDELIGLIPSLYDHYCSLGRPGGFLERLREGTWMGHVL
                     EHIALELQTLAGSEGTRGLTRSTDDRGVYNVVYQYAQHDLGMAAGELGVRLLNNLIYK
                     DDIEFDFSRELERLIRLAERLAYGPSTKALVDEAERRGVPVIRLDPRRSLVQLGYGIY
                     QKRIWATVTSETSNIAVDLAANKRLTNQLLQGVGIPVPRSATVESADEAVRAARSIGY
                     PVVLKPLDGNHGRGVRINLLDEEEVREAFQEAYDQSRNGVVAVESFIRGRDYRILVVG
                     GKLVACSERVPAHVIGDGVHTVQELIDITNSDPRRGIGHEKVLTRISVDAAVLDQLEK
                     CELTLESIPEAERFVQLRLTGNMSTGGISIDRTDEIHPDNIEIAEQAAQVIGLDVAGI
                     DFIVEDITIPVKDQGGAICEVNAGPGFRMHTHPTEGVPRDVARPVLDLLFPRGTPSRI
                     PIVAVTGTNGKTTTSRMISTILKMAGRRVGMTTTDGIYIDGTQILKGDMSGPHSAQMV
                     LQNPTVEHAVLETARGGILRAGLGFDRCDVAVVTNVTADHLGLAGIESLRDLARVKAV
                     VPAATFRDGYVVLNADDHWCVEMTSRSRGEVIFFSMDEENEKVKEHLRSRGKAVLLKR
                     VRDNEVIYLVEGKRETAVLDVRHIPATFEGRARVNIKNALAAVGAAWGSNIPLDAIRN
                     GLRTFTTSFFQAPGRLNLLEVGGYRVIVDYCHNVAGLQELAEFVRRMLPPRTVGMIAV
                     PGDRRDEDIRAFGTIAADMFDQFVIREDTNPRGRARGEVAGILHETLLASGVETERVS
                     VVLNEIEAAGAALDLARPDDLVVLLADKPAEVWETAVERSSRDWSNTAAGIRD"
BASE COUNT          673 a         1325 c         1324 g          710 t
ORIGIN      
        1 gccggctcag gcggctagcc tgcctgtcgc tgcgttgtca cgcggttgag gaagtcaaga
       61 acggtcgcgt cgaactcgga cggccagagg cggtggggca tgttgccgcc ccactcgtcc
      121 cgggtatggt caccggggaa gaggcgcaac tccgcgcctg ggatgcgtcc cgccagtgtc
      181 gtggcatgtt cgggcgggac cagcatgtcg tcgtgttgcc agacgatcag cgtcgggcag
      241 cgcagcaagg cggtctcggt ttcgatcacc cgcccgatca cctcggcatc gacgaggacg
      301 agcgcccgcg cgatctcggg atagcgggcg gcgacagtca ggcagatgcc gccacctgag
      361 gcggcgccga tcatgacggc gccctccggc gcggtcgccc ggatgaacgc ctcgatgaag
      421 cgcgggtagt agcggaagaa gtcgtcctcg gagtcgatcg gggccggtgg cgggccggat
      481 tcgcccagcc cgggcaggtc gggaatcagg acggtgtagt gacgcgacag cgcggccaga
      541 ttgtcccgcc acgtgtgcca cgagctctgc gtgccaccgg cgtggacgag cacgagcgcc
      601 gggccggcgc caatgcgcgc gtagtggacc cgtatgccgt tgatctcaac cgtttgttcg
      661 tcgaccatcg ccttcacccg gtaacccgaa aatgccaacc gtatcttcca ccataccatc
      721 gtacccgtgc gatagtactt tcgcgctgat ccgggcaatt cgcaagcgat cgctgcttgc
      781 ttcggccgcc cagatcgtat tggagagata cgccatgcca gatggcagcg agttccgcaa
      841 tcaggtgctg gaacgctacg atttcacccc agaagctcaa gcgatcctgc gacagattcc
      901 ggtcgagatt cacgacctga cccataccaa cggcggcggt ggctggtatc cgggtgagcg
      961 gcggatcatc ctcaacggcg tccaggatga agcctgcctg cacgagctgg cgcatgcctg
     1021 ggccgatgag actggcttct acgtcgatcc ccatccggat gaccctgacc gcaacggccg
     1081 gaacttcgcc taccgcaggg acgttgaccg ggccgcaatc gagtctgacc ctgcattcca
     1141 acgcgtcgcg ttcctggcct gggagtacac caatggcaac ccacgcaccg gcttcgaggg
     1201 catgaaggag atcgactggg agcgcttcgc cggcctggcg tcgggcgtga tgggcgacat
     1261 ccggctgatg ccgccctacc tgcgtcgctg gtacgagccg ctgttcggcg gtcatccaca
     1321 cgtaagcggt ccctcggaac tgccgtcgac cgtgccggtc aactggcggc agggtatcgg
     1381 tctggtcggg acgatgtggc cggcggcggc catgccgggc ggcggcgtgc tgacgctgct
     1441 cgaccgacta cgccgctggc tcacccgccg ttaaatcagt cgcggatgcc ggccgccgtg
     1501 ttgctccagt cacgcgaact acgttcgacc gccgtttccc agacctcggc cggcttgtcg
     1561 gccagcagga ccaccagatc gtccggacgg gccaggtcca gcgccgcgcc ggccgcctcg
     1621 atctcgttga ggacgaccga gacccgctcg gtctctacgc cactcgccag caacgtctcg
     1681 tgcaggatgc cggccacctc gccgcgcgct cggcctcgag ggttggtgtc ttcccgaatc
     1741 acgaactgat cgaacatgtc ggccgcgatc gtgccaaacg cgcggatgtc ctcgtcgcgc
     1801 cggtcacctg ggacggcgat catcccgacc gtgcgcggcg gcagcatccg ccggacgaac
     1861 tcggccagct cctgcaagcc ggcgacgttg tggcagtagt cgacgatgac gcgatagcct
     1921 ccgacttcga gcagattcag ccgccccggc gcctggaaga atgaggtcgt gaaggtccgc
     1981 agcccgttgc ggatggcgtc gagcgggatg ttcgagcccc aggccgcacc aacggccgcc
     2041 agcgcgttct tgatgttgac ccgtgcccgt ccctcgaagg tggctgggat atggcgtaca
     2101 tcaaggacgg ccgtctcgcg cttgccctcg acaaggtaga tgacttcgtt gtcgcgaacg
     2161 cgcttcaaca ggaccgcctt gccccgcgag cgcagatgct ccttgacctt ctcattctcc
     2221 tcgtccatcg agaagaagat cacctcgcca cgcgagcgcg atgtcatctc gacgcaccag
     2281 tggtcgtcgg cgttgaggac gacgtagccg tccctgaacg tcgcggccgg gacgacggcc
     2341 ttgacacgtg ccagatcgcg caacgattcg atgccggcca gccccagatg atcggcggtg
     2401 acgttcgtga cgaccgcgac atcgcagcgg tcgaagccga ggccggcccg caggatgcct
     2461 ccacgggcgg tttcgagaac ggcgtgctcg acggtcgggt tttgcagcac catctgggcg
     2521 ctgtgcggcc cgctcatgtc gcccttgagg atctgggtac cgtcaatgta gatgccatcg
     2581 gtcgtcgtca ttccgacccg gcgtcctgcc atcttgagga tggttgagat catccggctc
     2641 gtcgtcgtct tgccgttcgt gccggtcacg gccacgatcg ggattcgaga cggcgtcccg
     2701 cgcgggaaga gcagatcgag gacaggtcga gcgacgtcgc gcggaacacc ctcggtcgga
     2761 tgggtgtgca tccggaagcc gggaccggcg ttgacctcgc aaatcgcgcc gccctggtct
     2821 tttaccggaa tcgtgatgtc ctcgacaatg aagtcgatgc cggccacatc gagcccaatc
     2881 acctgggcgg cctgctcagc gatctcgatg ttgtcggggt ggatctcgtc ggtgcggtcg
     2941 atcgagatgc cgccggtcga catgttgccg gtcagccgga gctggacgaa tcgctcggcc
     3001 tcaggaatcg actccagtgt cagttcgcac ttctccagtt gatcgagaac ggccgcgtcg
     3061 acgctgatcc gggtcagcac cttctcgtgg ccgatgccgc gacgtgggtc gctgttggtg
     3121 atgtcgatca attcctggac cgtgtggaca ccatcgccga tgacatgcgc cggcacccgc
     3181 tcggagcagg caaccagctt gccgccgacg accaggatcc ggtagtctcg cccgcgaatg
     3241 aacgactcga cggcgacgac accgttgcgg ctctggtcgt acgcctcctg gaacgcttcg
     3301 cggacctcct cctcgtcaag gaggttgatg cggacgccgc ggccgtggtt gccgtccagc
     3361 ggcttgagga cgaccggata gccgatcgag cgcgccgcac ggactgcctc atcggccgac
     3421 tcgactgtcg cggaacgcgg gactggaatg ccgacgcctt gcagcagttg gttggtcagc
     3481 cgcttgttgg ccgccagatc gacagcgatg ttgctcgtct ccgaggtcac cgtcgcccag
     3541 atgcgcttct ggtagatgcc gtagccaagc tgaaccagcg aacggcgcgg atcgagccgg
     3601 atgacgggca ctccccgccg ctcggcctcg tccaccagcg ccttcgtgct ggggccgtag
     3661 gccagccgtt cggccaggcg aatcaggcgc tcaagctcgc gcgagaagtc gaactcgatg
     3721 tcgtccttgt agatcaggtt gttgagcagc cggacaccaa gctccccggc cgccatcccg
     3781 aggtcgtgct gtgcgtactg gtagacgacg ttgtagacgc cgcgatcgtc ggtcgagcgg
     3841 gtcaggccgc gcgtcccttc agaaccggcc agggtctgca gttccagcgc gatgtgctcc
     3901 aggacgtggc ccatccaggt accctcgcgc agccgttcga ggaatccacc gggccggccg
     3961 agcgagcagt agtgatcgta gagcgacggg atcaagccga tcaactcgtc gttgaagccc
     4021 tcgatcgtgt tc
//
LOCUS       DASUGJ010000079         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1677540, whole genome shotgun sequence.
ACCESSION   DASUGJ010000079 DASUGJ010000000
VERSION     DASUGJ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677540"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..709)
                     /locus_tag="VFV93_01815"
     CDS             complement(272..709)
                     /locus_tag="VFV93_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX5164105.1"
                     /translation="MTDEHGVERTLQILQDAGYRLTGPRRAVVDEIQRADRSFTADDL
                     LQRLTDSQSGVGRATVFRTLDLLVQNGVLDRLHQPDGCHSYLLCGVIDRHHHHLICSD
                     CGAVIEFEDCSVQPLLEELGRRTNFQINGHWLEVFGQCSVCRM"
     gene            783..2066
                     /locus_tag="VFV93_01820"
     CDS             783..2066
                     /locus_tag="VFV93_01820"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164106.1"
                     /translation="MKIEVDETSSPVATQAVEAEQSSETAVRSARHSLRNPQFLRFWI
                     AITLALTGLWVRITVQGYLVYDMTDDKFLLGLVGFLSALPVLLLSPVVGVVVDRFDRR
                     KVLFATQAFMATTLLTLATLDALGILAVYHILIIATLAGAASAFDWPARLSLVPNIVE
                     RDELQSAVAFNSASFNGARIVGPAVGGFLIGIIGTAACFYLSAFAFLPSMVVLMTLTI
                     DRSIPSGSVESPIQNLLSGYRYIWNFPTLRSLLSVDLVPVMFGMSFFALLPALTRDVY
                     GQGSQGLGLLYSADGAGAFAGVMIVATMTGLRHRGKLVLTFVTMFAVFLLLFALAPNI
                     WIGMILICVLGISSALYGTLADTLIQTIVNDQYRGRVMAVYSTFWGLTPIGYLQAGWV
                     ASKWGTQTAIFVNGCVVLAYVIALSIFNADVRKLD"
     gene            2088..2987
                     /locus_tag="VFV93_01825"
     CDS             2088..2987
                     /locus_tag="VFV93_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164107.1"
                     /translation="MIRRTFQRALQATRPPEPRRTINAMELPVATPSVAVSLARSLPS
                     ALGGLQLDLNRPVLLIPDQRDAGMPGSRVTDAVVRMLVAPGSISLGLPALLTTVERRR
                     WRGLAETAGIDVVTLGASGWDRIMLGDRSSILDAVDLPAELYDDVTVVALTTPAGAGA
                     AGVWTEFVHPNTRLRARVVEHGAIELAAAVDARYLMAGQFGTTWYAAIAAPVIVAEVL
                     ARAIERHHERSRGLEAPGPWEDAGVQHLAGLVPTGQNPYHLVLRTSLQDNADASLAQQ
                     ISEMLDWPLSFADDKEDQAGEAR"
BASE COUNT          500 a          960 c         1004 g          588 t
ORIGIN      
        1 ggcggaacgg gtcgaacggc gggagcggat cgactggcgc ttcacgatcg cggatgcgcg
       61 tgccaagatg cagcagtgtt atcccatccc aacgccctgt ccggacacgg cataatcacg
      121 ctggtcgact actgagccgc aacgaaggac ctctccacca tgacgccata cagggctgac
      181 acggagaggt ccttcgctgt agctcaggac gacagggtat tcgtgtacct gttgaccggt
      241 tgatcgccga ttgggacggg aagaaattcc gttacatccg gcagacggag cactggccga
      301 aaacttcgag ccagtggccg ttgatctgga agttggtgcg gcggccaagc tcttcgagca
      361 gcggctggac gctacagtcc tcgaactcga tcacggcgcc gcagtccgag cagatcaggt
      421 ggtggtggtg ccggtcgatg acaccacaca gcaagtatga gtggcagccg tccggctggt
      481 gtaggcgatc gagaacaccg ttctggacga gcaggtcaag cgtccggaag accgtcgcac
      541 ggccaacgcc ggactgcgag tccgtcagcc gttggagcag atcgtcggcg gtgaacgagc
      601 gatcggcgcg ctggatctcg tcgacgacgg cacggcgcgg gccggtcagg cggtagcccg
      661 cgtcctgtag aatctgtagc gtacgttcaa cgccgtgctc gtcggtcatc tgcctctccc
      721 ggctggtctg tcagccgtga gtgtaacatg gcatgtctga ccgatcgctt gcaggagcag
      781 ccgtgaagat tgaggtcgat gaaacgtcgt ccccggttgc aacgcaggcg gtggaggcgg
      841 aacaatccag tgaaactgct gtgcgttcgg cccgccattc cctccgcaat ccgcagtttc
      901 tgcgcttctg gatcgcgatc acgctggcgc tgaccggtct ctgggtgcgc atcaccgtcc
      961 agggctatct cgtctatgac atgaccgacg acaagttcct gctcgggctg gtcggtttcc
     1021 tgagtgcgct accagtcctg ctgctgtcgc ctgtcgttgg cgtcgtcgtt gaccgcttcg
     1081 accgccgcaa ggtgctgttc gcgactcagg cgttcatggc gacgacgttg ctgacgctgg
     1141 cgacgctcga tgcgctgggc attctcgccg tctatcacat cctgatcatc gcgacgctgg
     1201 ccggcgcggc gtcggcgttc gactggccgg cacgcctgtc gctggtgccg aacatcgtcg
     1261 aacgcgatga gctgcagagc gccgtcgcct tcaactcggc ctcgttcaac ggcgcccgga
     1321 tcgtcgggcc ggctgtcggc ggtttcctga tcgggattat cggcacggcc gcctgtttct
     1381 acctgtcagc cttcgccttc ctgccgtcga tggtcgtgct gatgacgttg acgatcgacc
     1441 gctcgatccc gagcggcagc gtcgaatcgc cgattcagaa cctgctttcg ggctatcggt
     1501 acatctggaa cttcccgacc ctgcggagtc tgctgtcggt cgatctcgtg ccggtcatgt
     1561 tcggcatgtc cttcttcgcg ctgctgccgg ctctcactcg cgatgtctac ggtcagggca
     1621 gccaggggct ggggctgctc tacagcgccg acggcgccgg agccttcgca ggagtgatga
     1681 tcgtcgcgac gatgaccggc ctgcgccatc gtggcaagct ggtgctgacg ttcgtgacga
     1741 tgttcgccgt cttcctgcta ctgttcgcgc tggcgcccaa catctggatc ggcatgatcc
     1801 tgatctgcgt tctcggcatc tcttccgcgc tctacgggac actggccgac accctgattc
     1861 agacgatcgt caacgaccag tatcgcggcc gggtgatggc cgtctattcg accttctggg
     1921 ggctgacgcc gatcggctat ctgcaggccg gctgggtcgc cagcaagtgg ggcactcaaa
     1981 cggcgatctt cgtcaacggc tgcgtcgtcc tcgcctacgt catcgccctg tcaatcttca
     2041 acgccgacgt ccgcaagctc gactgagccg gcagtcacgg agcaccgatg atccgcagaa
     2101 cattccaacg cgcgctgcag gccacacggc cgccggaacc acgacgcacg atcaacgcga
     2161 tggagttgcc ggtcgcgact ccgtccgtgg cagtcagcct tgcccggtcg ctgccatcgg
     2221 cgctgggcgg gctacagttg gacctgaatc gaccagtcct cctcattcca gatcagcgcg
     2281 acgccggcat gccggggtcg cgcgtgacgg acgccgttgt gcgcatgctc gtggcaccag
     2341 gctcgatatc gcttggcctc ccggcgttgc tgaccaccgt tgagcgtcgc cggtggagag
     2401 gactggctga gacggcgggt atcgacgttg ttacgcttgg cgcgtcgggc tgggaccgga
     2461 tcatgctggg agaccgatcc agtatcctcg acgcggtcga cctgcccgcc gagctgtacg
     2521 acgatgtgac ggtcgtggca ctcacgacgc cggccggggc gggggctgcc ggggtatgga
     2581 cggagttcgt ccacccgaac actcgcttgc gcgctcgcgt cgtcgaacat ggtgcgatcg
     2641 agcttgcggc tgccgtcgat gcacgctatc tgatggccgg gcagttcgga acgacctggt
     2701 atgcagccat cgccgcgccg gtcatcgtcg ctgaagtgct ggcaagggcc atcgaacggc
     2761 atcacgagcg atcgcgcggg cttgaagcgc cggggccatg ggaagacgcg ggcgtgcagc
     2821 acctcgccgg cctcgtgcca accgggcaga acccctatca tctggtcctg cgcaccagtc
     2881 tgcaagataa tgcggacgcg tcgctggcac agcagatcag cgagatgctg gactggccgc
     2941 tgtcgttcgc ggacgataag gaggatcaag cgggtgaagc acgatgatgg cctggcgccg
     3001 atgcggggcc gagtccagcc agagcgagcg tcggttgcgg cgctgagccg gc
//
LOCUS       DASUGJ010000080         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_566174, whole genome shotgun sequence.
ACCESSION   DASUGJ010000080 DASUGJ010000000
VERSION     DASUGJ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566174"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VFV93_01830"
     CDS             <1..1003
                     /locus_tag="VFV93_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5164108.1"
                     /translation="DLLVQAEEPEIGEPTAFEIATAMALLAFQRAGVDLAVVEVGMGG
                     RLDATNVVTPDVAAISAISYDHTAILGATLEKIAFEKGGIIKPGKPVVVGPQPAEALA
                     ELRRIASERAAPMFLAGRDWETEATDAGATVSGPWGDWREVELALAGAHQVENAGLAL
                     MALWLLDPALLADESRVRAALASVRWPGRFERIATAPDVYVDGAHNVDSIERLAETVR
                     PLIGDGRRLHLILGISRDKDIDGIVAALAPLAPIVTATASHNPRAAGPDQIAAVATAA
                     GLTATTAPSVDAALDAARATAAPTDTILVTGSLYAVAEAREALGLAETPTFERELLY"
     gene            1226..2713
                     /locus_tag="VFV93_01835"
     CDS             1226..2713
                     /locus_tag="VFV93_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX5164109.1"
                     /translation="MSQSSRTMNAPGEPASGGWDAMLQRYFRFREMGSDTRTEIMAGL
                     TTFVVMSYIIFVNPGILSTPGPENSGIAGLDISATTTSTCLVAGVMTILMGLFANKAY
                     AIAPGLGLNAIVAFTLVGQEQLTYAQAMGVIVAEGILITALVLLGVRRYVMDLVPIEL
                     KKAISVGIGLFILFIGLVNGGLVRRTPFEGGTPLEIGDLHGAPVLVTAVGLALTIALI
                     ARGYRAAILLGIVGTTVFAIVLREVFDKAGFVGTQASFPDKVIDTPDFSLLGDFSFKF
                     FSEMGVTVAILTILAIMMADFFDTMGTLVGVGSQAGYLNEKGELPDAQKPLLVDSLAA
                     IAGGAASSSSATTYIESASGVANGGRSGLVSVVTGILFLLAMPFAPLVSIVPTQATAP
                     ALIVVGWMMMSVLSEREEVTGDGRIVRARGIDFADIEVGLPVLATMVMMPLTYNITNG
                     IGAGFVVWTLVKLFRGKASEIHPVMYVISAAFLIYFLRSYLGVDA"
     gene            complement(2809..>3033)
                     /locus_tag="VFV93_01840"
     CDS             complement(2809..>3033)
                     /locus_tag="VFV93_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164110.1"
                     /translation="GTDRREKLFAYRQIETLRSYLIVHADEKVIEQHWIDNGVWRHDI
                     LEDNASVHIPCLDFDLDLADIYEGVTFEES"
BASE COUNT          487 a          952 c         1027 g          567 t
ORIGIN      
        1 cgacctgctc gttcaggccg aggagccgga gatcggtgaa ccgacagcat ttgagatcgc
       61 gacggcgatg gcgttgctgg ccttccagcg ggccggcgtc gacctggccg tcgtcgaggt
      121 cgggatgggc ggtcggctgg acgcgaccaa cgtcgtgacc ccggacgtgg ccgccatcag
      181 cgccatctcc tacgaccaca ctgcgatcct cggcgcgacg ctggagaaga tcgcgttcga
      241 gaagggcggc atcatcaagc cgggcaagcc ggtcgtcgtt ggtccgcagc cggctgaggc
      301 gctggcggag ctgcgccgga tcgcgagcga gcgcgcggcg ccaatgttcc tggctgggcg
      361 cgactgggaa acggaggcaa ccgacgccgg cgcaacagtg tcgggaccgt ggggcgactg
      421 gcgcgaggtg gagctggcgc tggctggggc gcatcaggtc gagaacgccg ggctggcgct
      481 catggcgctc tggctgctcg atccggcgct gctggcggac gagagccgcg tccgggcagc
      541 gctggcgagc gttcgctggc cgggccgatt cgagcggatc gcgaccgcgc cggatgttta
      601 cgtcgacggt gcccacaacg tcgactcgat cgagcgactg gcggagacgg ttcgcccgct
      661 gatcggtgac ggccggcgcc tgcacctcat cctcggtatc agccgcgata aggacatcga
      721 cggcatcgtc gccgccctgg cgccactcgc cccgatcgtc acggcgaccg cctcgcacaa
      781 cccgcgtgcc gcaggaccgg accagatcgc ggccgtcgcc acggcggccg gcctgaccgc
      841 gacgacggca ccgagtgtcg acgctgcgct cgacgccgcc cgcgcgactg ccgctccaac
      901 cgacacgatc ctcgtcactg gctcgctcta cgccgtcgcc gaagcgcgcg aagcgctggg
      961 tttggccgag acgccgacct tcgagcggga gttgctgtat tagtcccgaa aacccctccc
     1021 ccacggagag tggcttttga ttcgtgcagc ctgaagcccc tctccatggg gaggggtgtt
     1081 cgacatgccc cacgccaatt gacgggatgt catcgggata ctacgatgat agcgtacgta
     1141 cgcattggga gcctcgacgg tttgggggcc gggctttcag cgtcgtcgtg cgttgggatg
     1201 aacgtgtgga tgggggtccg cgcctgtgag ccagtcgtcc agaacaatga acgcgcccgg
     1261 tgagccggca tccggtggct gggacgcgat gttgcagcgc tatttccgct tccgcgagat
     1321 gggctcggat acgcggacgg agatcatggc cgggctgacg acgtttgtcg tcatgtcgta
     1381 catcatcttc gtcaaccccg gcatcctctc gacgcccggg ccagagaact cgggcatcgc
     1441 cggattggac atctcggcta cgacaacatc cacctgtctg gtggctgggg tgatgacgat
     1501 cctgatgggg ttgttcgcca acaaggccta tgcgattgcg ccgggcctcg gcctcaacgc
     1561 aatcgtcgca tttacgctcg tcgggcagga gcaactgacc tacgcacagg cgatgggtgt
     1621 gatcgtcgcc gagggcatcc tcatcacggc gctcgtcctg ctgggtgtac ggcgttacgt
     1681 catggacctc gttccgatcg agctcaagaa ggcgatctcg gtggggatcg gcctcttcat
     1741 tctgttcatc ggccttgtca acggtgggct cgttcgacga acgccattcg aaggtggaac
     1801 gccgcttgag atcggtgatc tgcatggcgc gccggtgctg gtgactgccg ttgggctggc
     1861 gctcacgatc gcactgattg cgcgcggcta tcgggccgcg atcctgttgg gcatcgtcgg
     1921 gacgaccgtc ttcgcgatcg tgctgcgcga agtctttgac aaggccgggt tcgtcggaac
     1981 ccaggcgtcg tttcccgaca aggtgatcga cacgccggac ttcagcctgc tgggcgactt
     2041 ctcgttcaag ttcttctccg agatgggcgt cacggtcgcg atcctgacga tcctggcgat
     2101 catgatggcc gacttcttcg acacgatggg gacgctggtc ggggtcggca gccaggccgg
     2161 ctacctgaac gagaagggtg agctgccgga cgcccagaag ccgctgctgg tcgactcgct
     2221 ggcggccatc gccggtggcg cggcatcgtc gtcatcggcg acgacctaca tcgagtcggc
     2281 atccggcgtt gccaacggcg gccggagcgg gctcgtctcg gtcgtgactg ggattctgtt
     2341 cctgctggcg atgccattcg cgccgctggt gtcgatcgtt ccgacccagg cgacggcgcc
     2401 ggccctgatc gtcgtcggct ggatgatgat gagcgtcctc tccgagcgtg aagaggtgac
     2461 cggcgatggc cggatcgtcc gcgcgcgggg catcgacttc gctgacatcg aggttggcct
     2521 gccggtcctg gccacgatgg tgatgatgcc gctgacctac aacatcacca acggcattgg
     2581 cgccggcttc gtcgtctgga cgctggtcaa gctcttccga gggaaggcca gcgagatcca
     2641 cccggtcatg tacgtgatca gcgcggcatt cctgatctac ttcctgcgct cgtatcttgg
     2701 ggtcgatgcg taagtcgcag cctcgcgtcg gggcgatggc cgcagcctcg ccctgcccag
     2761 atcgcacgaa caagggcgag gctgcggcca tcaccccgac gcgaggggtc agctttcttc
     2821 gaaggtgacg ccctcgtaga tatcggcaag atccagatcg aagtcgagac atggaatatg
     2881 gacagaagcg ttgtcttcca ggatgtcatg tcgccagacg ccgttgtcga tccaatgctg
     2941 ctcgatcacc ttttcatccg catggacaat caggtagctg cgcagcgttt cgatctgccg
     3001 atacgcgaag agcttctctc tccgatcagt acc
//
LOCUS       DASUGJ010000081         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1121687, whole genome shotgun sequence.
ACCESSION   DASUGJ010000081 DASUGJ010000000
VERSION     DASUGJ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121687"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="VFV93_01845"
     CDS             complement(<1..1270)
                     /locus_tag="VFV93_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5164111.1"
                     /translation="MTPTSTYRIQFNKDFPFANAEEIVLYLRELGITTLYASPLFKAR
                     SGSAHGYDVTDPGTMNPELGGEDGFAALSDTLRQAGMTLLLDIVPNHMAASPENRWWL
                     HVLENGPSSEYANYFDIVWQTSASGEPLESKVVLPILGSHYGTVLENQELQIVRDGGE
                     FYVAYWDNRLPLDPKTYRVVLEQRYGILRDRLSNHPQAYREYEALIELTEEMPDRLTV
                     DPELVERRRNGTTRLKAELERLCESYPEINEFVEENLRLVNGTPGDPQSFDTLDHLIS
                     AQAYRLAFWRVANAEINYRRFFDIADLVSMRIEDEDVFRARHERLIDLFGNGRLHGLR
                     IDHIDGLHDPQGYLERLQAFLAPARAELATGENESYILVEKILAEHEHLREDWPTVGT
                     TGYDFLNLVTGLLIDGSATEALTEIYQRVSG"
     gene            complement(1368..>2113)
                     /locus_tag="VFV93_01850"
     CDS             complement(1368..>2113)
                     /locus_tag="VFV93_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX5164112.1"
                     /translation="YRQRRHGNSPTQAAARQFVVASQNHDQVGNRAIGEWLAHLVSFE
                     SAKLAAATYLLSPYIPLIFMGEEYAESAPFQYFTSHTDPELAKAVSEGRRTEFSRFEW
                     ADEVPDPQDEATFQRSKLDHTLRESGRHKAINDLYRELLRLRRELPALAHLSKDDQQV
                     IAYEVEQVLYVHRWHAEIEVVLALNFAETPVEIMLPIPAGDWRRVLDTADERWGGEGV
                     ETAESVESNGRVRLRLAERSAVMYQRHDG"
BASE COUNT          339 a          686 c          675 g          413 t
ORIGIN      
        1 tacccgagac gcgctggtag atctcggtta gcgcctcggt cgcagagccg tcgatcaaca
       61 gcccggtgac gaggttgagg aagtcgtagc cggttgtccc gaccgtcggc cagtcctcgc
      121 gcaggtgttc gtgctcagcc aggatcttct cgaccaggat gtaactctcg ttctcgcccg
      181 tcgccagctc cgcccgggcc ggtgccagga aggcttgcaa ccgttcgaga tagccctgcg
      241 ggtcgtggag gccgtcgatg tggtcgatcc gcagcccgtg cagccggcca ttgccgaaca
      301 ggtcaatcag ccgctcgtgc cgggcgcgga agacgtcctc gtcctcgatc cgcattgaga
      361 ccaggtcggc gatgtcgaag aatcggcggt agttgatctc cgcgttggca acccgccaga
      421 aggccagccg gtacgcctgg gcggagatga ggtggtcgag cgtgtcgaac gactgcgggt
      481 cgccgggtgt gccgttgacg agccgcaggt tctcctcgac gaactcattg atctccgggt
      541 agctctcgca gaggcgttcc agctccgcct tcagccgcgt cgtcccgttc cgtcgtcgct
      601 cgaccagttc cggatcgacc gtcaatcggt cgggcatctc ctcggtcagc tcgatcagcg
      661 cctcgtactc ccggtacgcc tgcggatgat tactgagccg gtcgcgtagg atgccgtagc
      721 gctgctccag cacgacccgg taggtcttag ggtcgagtgg cagccggttg tcccagtagg
      781 caacgtagaa ttcgccgccg tcgcggacga tctgcagttc ctggttctcc agcacggtcc
      841 catagtggct accgaggatc ggcaggacga ccttgctctc cagcggctcg ccgctggcgc
      901 tggtctgcca gacgatgtcg aagtagttgg cgtactcgga gctcggcccg ttctccagca
      961 cgtgcagcca ccagcgattc tccgggctgg ccgccatgtg gttcgggacg atgtcgagca
     1021 gcagtgtcat cccagcctgc cgcagcgtgt cagacaatgc cgcgaacccg tcctcgccgc
     1081 ccagctctgg gttcattgtc cccggatcgg tcacgtcgta cccatgcgcg ctacccgacc
     1141 gcgccttgaa caacggcgag gcgtacagag ttgtgatccc aagttcgcgg aggtacagca
     1201 cgatctcttc ggcgttggcg aagggaaagt ccttgttgaa ctggatgcga taggtcgatg
     1261 ttggtgtcat cgaattcgct ggctcccaat ggtcatcgca tcgtactgcg gacctgcaaa
     1321 ctcaagcgat ccaggcgtcc tcagccacat cggacgcctg gatcgcttca gccatcgtgg
     1381 cgctgataca tgaccgccga ccgttccgcc aaccgcaggc gaacacgccc gttcgattcg
     1441 accgattctg ccgtctcgac accctcgccg ccccaacgct cgtctgccgt gtccagcacc
     1501 cgccgccagt cgccggccgg aatcggcaac atgatctcga caggtgtctc ggcgaagttc
     1561 agcgccagca cgacctcaat ctcggcgtgc cagcggtgga cgtacaggac ctgctcgacc
     1621 tcgtaggcga tgacctgctg gtcgtccttc gataggtggg ccagcgctgg tagctctcgc
     1681 cgcagccgga gtagttcgcg gtaaagatcg ttgatcgcct tgtgccggcc ggattcccgc
     1741 aacgtgtggt caagcttcga gcgctggaag gttgcttcgt cctgcgggtc cggcacctcg
     1801 tctgcccact cgaagcggct gaactcggtg cggcggccct cggagacggc cttggcgagt
     1861 tccgggtcgg tgtggctggt gaagtactga aacggcgccg actcggcgta ctcctcgccc
     1921 atgaagatca gcgggatgta cggcgagagc aggtaggtcg ctgccgccag cttggccgac
     1981 tcgaatgaga cgaggtgcgc cagccactca ccgatcgcac ggttgccgac ctggtcgtgg
     2041 ttctggctgg cgacgacgaa ctgccgtgcc gccgcctgcg tcggtgagtt gccgtgccgt
     2101 cgctggcggt act
//
LOCUS       DASUGJ010000082         3578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_149831, whole genome shotgun sequence.
ACCESSION   DASUGJ010000082 DASUGJ010000000
VERSION     DASUGJ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149831"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VFV93_01855"
     CDS             complement(<1..877)
                     /locus_tag="VFV93_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX5164113.1"
                     /translation="MATDDNRLLGVVTDGSFSAGLTVRLDGDAGEDLQVGSFVVLEGN
                     NRRYFSLINDLQLRATDPGVISDPPIDSAFIRDALRGIHTYTAAMVRPSLVMDDAESI
                     SGDARPRAVRTIPTHFARMRQAEERDFDLVFGAEGDDLFAVGSPIAMDGVKVTIDLKK
                     LVERPSGVFGQTGTGKSVLTRLILFGIIRSDIASALVFDMHDEYADEKHDKPGIPGLR
                     DLFDSTRVKVFSLDTSNTKATYHISIGMNQIEPADIELLSDELNLSDTFSSTAFLLRR
                     HFGDQWLSRLLAMDGV"
     gene            1090..2997
                     /locus_tag="VFV93_01860"
     CDS             1090..2997
                     /locus_tag="VFV93_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5164114.1"
                     /translation="MSEKPASPIDALAAMMAAVEQDASTDGGPARRAAHRMGEACAAS
                     DLTIEETFEILEREAGEALTDFNAEVLGSIVRGYVDQVVRVRGLTRRRGTGRLSSHVS
                     RLAALHRINRAATASLRLDEMLSTVVEVIAETIDGDACSVFLADPSAETLMLRATVGL
                     NPEAIGRLAIRSDAGITGLAASSRQTQIAVNASQHPAYFTYPIVGEDRYTSQVSIPLL
                     LREPERLVGVLNIQSLEERIFDQDEITFLETAAGELSIAIENARLYSQTDAALHRRIS
                     ELHQLRSVARSLTSMLRSDELLPLIASQAATIMSGNAATIARVLDDGDTSRIDFLASF
                     PESDGDQQHFPDELIRTVYSSRTAIVMPESGDDETHNVVLGAPLMTGHGVFGAVCVRL
                     DAAENHTDDKLGLFQSYADSAALALENAALYDEARRGYSTASTLLQEMHHRVRNNLQT
                     VAALLSMQARHARYAEWTAPLQEAVARVQSIAAVHSLLSSDNVTSATIASIVKHVVDE
                     ASINVVPPGVSVTFNIEPSDIEASSRQATILALLINECVTNAIEHGMEGRERGKIDVS
                     IHQQDGTIEVVIEDDGNGLTEGFSVEHNSRLGLRIAQTLASSDLGGSYSLQSRPGGGA
                     SSIIRFPAWQR"
     gene            complement(3055..>3578)
                     /locus_tag="VFV93_01865"
     CDS             complement(3055..>3578)
                     /locus_tag="VFV93_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX5164115.1"
                     /translation="DAVLVNAAGCGAAMREYDFLLRNDPAYAEQSRRFSDGVQDVTEF
                     LVAQGLTATPGRLDLTVTYQEPCHLVHAQRVALQPRALLAAIPGLTLVEMPESSLCCG
                     SAGIYNLLQPDMSSTLLERKLTNAASTGAPVIVSANPGCMLQLQAGLRERGVETEVVH
                     IMSLLDRAYAAAE"
BASE COUNT          648 a         1253 c         1085 g          592 t
ORIGIN      
        1 tcacgccatc catcgccagc aaccgcgaga gccactgatc gccgaaatgg cgtctgagca
       61 gaaacgctgt cgatgagaag gtgtcgctca agttcagctc gtcgctcagc agctcgatgt
      121 cagccggctc gatctgattc atgccaatcg agatgtggta ggtcgccttc gtgttgctcg
      181 tatccagcga gaagaccttg acccgcgtcg agtcgaacag gtcgcgcagg cccggaatgc
      241 ccggcttgtc gtgcttctcg tccgcgtact cgtcgtgcat gtcgaagacc agcgccgagg
      301 cgatgtcgct gcggatgatc ccgaacagga tcaagcgtgt caggacgctc ttaccggtcc
      361 cggtctgccc gaagacaccg ctgggccgct cgaccagctt cttcaggtcg atcgtcactt
      421 tcacgccatc catcgcgatc ggcgaaccga ccgcgaagag gtcatcgcct tcggcgccga
      481 agacgaggtc gaagtcccgt tcctccgcct gtcgcatgcg cgcgaagtgg gtcgggatcg
      541 tccgaaccgc gcgcggcctg gcgtcaccgc tgatgctctc cgcgtcatcc atcaccaggc
      601 tgggccggac catcgccgcc gtgtaggtgt ggatgccgcg cagtgcgtcg cggatgaagg
      661 ctgagtcgat cggcgggtcg gagatcacac cgggatcagt cgcgcgcaac tggagatcgt
      721 tgatcaggct gaaatagcga cggttgttgc cctccaggac gacgaagctg ccgacctgca
      781 aatcctcgcc ggcgtcgcca tccagccgca ccgttagccc ggcgctgaag ctgccgtcgg
      841 ttaccacgcc cagcagtcgg ttgtcgtctg ttgccacccg cgaccagctt tccagagtgt
      901 ccgtacgccc ctcgattgta tcatccgctc agatcacgac tcaccgctgc ctgaaggtct
      961 gactgcctgc tgacatcgct gaatgcgtaa catgattggt cggcgcgcga cgacgcccca
     1021 ccgctcaggg tatcctcggg atcggcatcc ggcggaccga gacaggatcg atcgtgagta
     1081 cgacagacga tgagtgagaa accggccagc ccaatcgatg cactggctgc gatgatggcg
     1141 gcggtcgagc aggatgcgtc caccgacggc ggccccgccc ggcgcgcggc gcatcggatg
     1201 ggcgaggcct gcgcggcgtc ggacctgacg attgaggaga ccttcgagat tctcgaacgc
     1261 gaggcgggcg aagccttgac cgacttcaac gccgaggtgc tgggctcgat cgtgcgcggc
     1321 tacgtcgatc aggtcgtccg cgtccgtggc ctcacgcggc gacggggaac cggccgcctc
     1381 tccagccacg tctcgcggct ggccgcgctc caccgcatca accgggcagc gaccgccagc
     1441 ctgcggctcg atgagatgct ctcaaccgtc gtcgaggtca tcgccgagac gatcgatggc
     1501 gacgcctgct cggtgtttct ggccgatcca tcggccgaga cgctgatgct gcgtgcaacc
     1561 gttggactca accccgaggc gatcggtcgc ctggcgatcc gcagcgacgc cggcattacc
     1621 ggcctggccg cgagcagccg ccagacgcag attgccgtca acgccagcca gcaccccgcc
     1681 tacttcacct acccgatcgt cggtgaggac cgctacacgt cgcaggtctc gatcccgctg
     1741 ttgctgcgcg agccggaacg gctggtcggc gtcctcaaca tccagtcact cgaagaacgg
     1801 atcttcgatc aggacgagat caccttcctc gaaaccgccg ccggcgagct ctcaatcgcg
     1861 atcgagaacg cgcgcctcta cagtcaaacc gacgccgccc tccatcgtcg catctccgag
     1921 ctgcaccagc tccgttcagt cgcccgctcg ctgaccagca tgctgcgttc cgacgagttg
     1981 ctgccgctga tcgcctccca ggccgcgacg atcatgtccg gcaacgccgc gacgatcgcc
     2041 cgcgtcctcg acgacggcga cacctccaga atcgactttc tggcgtcatt tccagagtcc
     2101 gacggcgatc agcaacactt ccccgacgaa ctcattcgaa cggtctactc atcgaggacg
     2161 gccatcgtca tgcctgaatc tggcgacgac gagacgcaca acgtcgtcct cggcgcgccg
     2221 ttgatgacgg ggcacggcgt cttcggcgcc gtctgcgtac gcctcgacgc agccgagaac
     2281 cacaccgacg acaagctcgg cctgttccag tcgtacgccg actcggccgc gctggccctc
     2341 gaaaacgcgg cactctacga cgaggcccgg cgcggctact ccaccgcctc gacgttgctt
     2401 caggagatgc accaccgtgt ccgcaacaac ctgcagaccg tggcagccct gctctcgatg
     2461 caggcccgcc acgcgcgcta cgccgagtgg accgccccgc tacaggaagc tgtcgcccgc
     2521 gttcagtcga tcgcagccgt ccacagcctg ctctccagcg acaacgtaac gtcggccacg
     2581 atcgcctcga tcgtcaagca cgtcgttgac gaggccagca tcaacgtcgt cccgcccggc
     2641 gtcagcgtca ccttcaacat cgagccgagc gacatcgagg cgtcctcacg gcaggcaacc
     2701 attctggcgc tgctcatcaa cgagtgcgtc accaacgcaa tcgagcacgg catggaaggc
     2761 cgcgagcgtg ggaaaatcga cgtctcgatc caccagcagg acggcacgat cgaagtcgtc
     2821 atcgaggacg acggcaacgg cctcaccgaa gggttctcgg tcgagcacaa cagccggctg
     2881 ggcctgcgca tcgcccagac tctcgcctcg tcggatctcg gcggctccta cagccttcaa
     2941 tcccgccccg gaggcggagc cagctcgatc atccgcttcc cggcctggca gcgctgacaa
     3001 catccggatc tcggaatcgg ccccgagccg accacgttca ggcgaacggc gggatcattc
     3061 agctgccgcg tacgcccgat ccagcagcga catgatgtgc acgacctcag tctcgacacc
     3121 acgctcgcgc aagcctgcct gcaactgcag catacagccc gggttggccg agacgatcac
     3181 cggcgcacct gtgctcgcgg cattggtgag cttgcgttcc agcagcgtcg aagacatgtc
     3241 cggctggagc aggttgtaga tcccggcgct gccgcagcac agcgacgact caggcatttc
     3301 aaccagtgtc agccccggta tcgccgccag cagcgctcgc ggctggagcg cgacccgttg
     3361 ggcgtggacg agatggcacg gctcctggta tgtcacggtc aggtcgagcc gccccggcgt
     3421 cgccgtcagt ccttgcgcga cgaggaattc ggtcacatcc tggacgccat cggagaaccg
     3481 gcgcgactgc tcggcgtagg ccgggtcgtt gcgcagcagg aagtcgtact ccctcatcgc
     3541 ggcgccgcag ccagcagcgt tgacgagaac ggcgtcga
//
LOCUS       DASUGJ010000083         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1540086, whole genome shotgun sequence.
ACCESSION   DASUGJ010000083 DASUGJ010000000
VERSION     DASUGJ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540086"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..2156
                     /locus_tag="VFV93_01870"
     CDS             177..2156
                     /locus_tag="VFV93_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX5164116.1"
                     /translation="MEDGRRRVVIEGVYPEIDGGRFPIKRTVGEAVVVEADAYTDGHD
                     AISLILLHRRDGDKHWSEVPMTPLVNDRWRGEFQVHDLGRACYTLTGWVDRFKTWTRD
                     LRKRLDAGQDVAVDLLIGAQLIEEAAANAPEADAASMRGVAEALRANQPGASERVFTP
                     ELTWLMDRYAERHFATRYDRELAVTVDPVLARFSTWYELFPRSASLEPGRHGTFADVE
                     AQLPEIAAMGFDVLYLPPIHPIGRSFRKGANNTTTAEPGDPGSPWAIGAEEGGHNAIH
                     PELGTLENFRRLVTSAREHGLELAMDIAFQAAPDHPYVTKHRNWFRERPDGTIQYAEN
                     PPKKYQDIYPFDFESDDWRGMWEELKSVFTYWCEQGVRVFRVDNPHTKAFPFWEWCIA
                     GVKAEYPETIFLSEAFTRPKVMYRLAKAGFTQSYTYFTWRNTAWELREYLTELTQTEV
                     AEYFRPNFWPNTPDILPEYLQYGGRPAFLTRLILAATLTANYGMYGPAFEALDATPLA
                     EGREEYWESEKYQIRHWPIEPRRRMREIITIVNRIRRENPALQSNRTLAFHDIDNDQI
                     LAYSKTTDDGANLILTVVNLDPHHVQSGWLELPLEQLGIEPDQPFQVHDLLGGGRYMW
                     HGHRNYVELDPHAMPGHIFHILRRVKREQDFDYFM"
BASE COUNT          424 a          696 c          724 g          359 t
ORIGIN      
        1 gcggttcatg ccagagatcg tagcaggggc gtcaatcaca gccgtcgcca cggggaatca
       61 ccattcccac aatggcacgg ctcatgcacg ctatccttgt ctggaacgtg caggtacggc
      121 ggatgcttgt cgcccgcgtc attcggacga agcgacgagg cagggaaggg gctggcatgg
      181 aggacggtcg tcggcgagtc gtgattgagg gggtctaccc ggagatcgac ggcggccgct
      241 ttccgatcaa acgaacggtc ggcgaggcgg tcgtagttga agcggacgcc tacaccgacg
      301 gccacgacgc catctcgctc atcctgctgc atcgccgtga cggtgacaag cactggtccg
      361 aagtgccgat gaccccgctc gtcaacgatc gctggcgggg cgagtttcag gtccatgacc
      421 tgggccgcgc ctgctacacg ctcaccggct gggtcgatcg cttcaagact tggacgcgcg
      481 atctgcgcaa gcggctggat gccggtcagg acgtggccgt cgacctgctg atcggcgccc
      541 aactgatcga ggaggcagct gccaacgcgc cggaagccga cgccgcgtcg atgcgggggg
      601 ttgcggaggc gctacgggcc aaccagccgg gagcatccga gcgggtcttc acgccggaat
      661 tgacctggct gatggaccgg tacgccgagc ggcatttcgc gacgcgctat gaccgggagc
      721 tggcagtgac ggtcgatccg gtcctggcgc gcttctcgac ctggtacgag ctgttccccc
      781 gctcggcctc actggagccg gggcggcacg gcacctttgc cgatgtcgaa gcgcaactgc
      841 cggaaatcgc ggcgatggga ttcgatgtcc tctacctgcc gccgatccac ccgatcgggc
      901 gctcgttccg caagggcgcc aacaacacga cgacggccga accgggcgac cccggcagcc
      961 cgtgggcgat cggcgccgag gagggcggcc acaacgcgat ccacccggaa ctcggcacgc
     1021 tggagaactt ccggcggctg gtcacaagcg cccgtgagca cgggctggag ctggcgatgg
     1081 atatcgcgtt ccaggccgcg ccggaccatc cgtacgtgac gaagcatcgc aactggttcc
     1141 gcgagcgacc ggacggcacg atccaatacg ccgagaaccc gccgaagaag tatcaggaca
     1201 tctacccgtt tgacttcgaa tccgacgact ggcgggggat gtgggaagag ctgaagagcg
     1261 tcttcacgta ctggtgcgag cagggtgtgc gcgtcttccg ggttgataat ccgcacacga
     1321 aggcgttccc attctgggag tggtgcatcg ccggcgtcaa ggcagagtat cccgaaacga
     1381 tcttcctgtc cgaggcgttc acgcggccga aggtgatgta ccggctggcg aaggccggct
     1441 tcacgcagtc gtacacctac ttcacctggc gcaacacggc ctgggagctg cgcgagtacc
     1501 tgaccgagct gacgcagacc gaggtcgctg agtatttccg gccgaacttc tggccgaaca
     1561 cgcccgatat cctgccggag tatctgcaat acggcggccg gccggccttt ctgacacggc
     1621 tgatcctggc ggcgacgctg acggccaact acgggatgta cggcccggcc ttcgaggcac
     1681 tggacgcgac accgctggca gaggggcgtg aggaatactg ggaatcggag aagtaccaga
     1741 ttcgccactg gccgatcgag ccacgccgcc gcatgcgcga gatcatcacg atcgtcaacc
     1801 gcatccggcg tgagaacccg gccttgcaat cgaaccggac gctggcgttc cacgacattg
     1861 acaacgacca gatcctcgcc tattccaaga cgaccgatga cggcgcgaac ctgatcctga
     1921 ccgtcgtcaa cctcgacccg caccacgtcc agtccggctg gctggagcta ccgctggaac
     1981 agctcgggat cgaaccagac cagccattcc aggtccacga cctgctgggc ggcgggcgct
     2041 acatgtggca cggccaccgg aactacgtcg agctcgaccc gcacgcgatg ccggggcata
     2101 tctttcacat tctccgacga gtcaagcggg aacaggattt tgattatttc atgtgagcgg
     2161 gaaccgggaa tcgggattcg ggggtaggta acgtcgtaca ttc
//
LOCUS       DASUGJ010000084         3457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1400257, whole genome shotgun sequence.
ACCESSION   DASUGJ010000084 DASUGJ010000000
VERSION     DASUGJ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400257"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="VFV93_01875"
     CDS             <1..1431
                     /locus_tag="VFV93_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5164117.1"
                     /translation="DVLRIAWPSILENLLQSILGVITIFMVSQLGSSEVAAVGASQQL
                     QMLFISAFFALSMGATVIVAHAYGARQHDAIGMAAKQATVATIVLSSIIAVIVFALAE
                     PMLRWMGADSDVIAQGTRFQQISAVGYVFMALMFVLGGALRGVGDTRTPMLVTLGINV
                     INVAVAWPLIFGKLGMPQLGLDGAAIAQNISRFAGFVVLAWLLYRGNRGVSIAGRDGW
                     RPNVTFLKRLADISIPAMAESLLRGGGQILFVVVVFMLGTAVVAGHNIAQQAVFLSMF
                     PGFGFAMAATALTGQSLGAGNPARARAATLTATRACIVWMSLMGVLFFVFAEPIMEFS
                     APDEAGRQQIIDSGVAALRVIAFAQPFQAIGFVLAGALRGGGDTRFPMFSTGISMWIF
                     RIPLAYLFAITADLGLAGVYLAMGTDNAILMALNIWRYRQGKWAENRITVTEEPDHSA
                     EPTTPPPTLLPAPEPDPVAAGGDAAE"
     gene            1428..1943
                     /locus_tag="VFV93_01880"
     CDS             1428..1943
                     /locus_tag="VFV93_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164118.1"
                     /translation="MNDVDALIRALIHGERQPADDDATTILEHIAGSTFYPGRRNIPR
                     PLQELMIRHGYEVPDRGDDLIYHWAKHVLADQQWTVDTTATAYFQFAVRAVTHPASRL
                     LVQFAPSGHRVAVVISAVDDIVPLASQGARSGRNLLVVYSADVGRIVSAHMTASTSRI
                     TSRTGTVWLRR"
     gene            1928..2269
                     /locus_tag="VFV93_01885"
     CDS             1928..2269
                     /locus_tag="VFV93_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164119.1"
                     /translation="MATQVTSKIQEDIDWYMAALEREWKSAVALVANWDSADPFDKDD
                     LAIEWHLTIDFLLRVLKYQECGLMSAEQQQRFTEIRQFMRDHEAEIDAVLGPGEAMVD
                     PPREWYVSLEQ"
     gene            2291..2941
                     /locus_tag="VFV93_01890"
     CDS             2291..2941
                     /locus_tag="VFV93_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein"
                     /protein_id="HEX5164120.1"
                     /translation="MSRIEQPPFALRLLMLIGGLFVFALAMALSLQCNLGAASWTVFH
                     DGISRQTPLSIGMATQLVGLLMLGVSWLAGIRPGFGTVANMILVGLFLDLILWTGVIP
                     EADAYPLRVAMLLAGIVVLGLASALYIKAGFGAGPRDSFMLAVHQRTGLRIGKVRWMM
                     ESGAVVAGIILGGAFGVGTIIFACLVGFSVDFFFNLLGVRTRPKPEAASPTPMAAD"
     gene            3020..>3457
                     /locus_tag="VFV93_01895"
     CDS             3020..>3457
                     /locus_tag="VFV93_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164121.1"
                     /translation="MSGSDQGQHSDPSAGTGTGEVNLETGGGAAVEVEEASAPTALST
                     ETPVARLEAMRARVMPILQSIKTEYTGRTRNGYPTIVDNIQPGGVFGLNLDPGWAIYF
                     MTDGTSLFAELHKTSLRTDTLSMANVEKFAGRPSIDRRDIDETW"
BASE COUNT          562 a         1111 c         1124 g          660 t
ORIGIN      
        1 gatgtcctgc ggatcgcgtg gccctcgatc ctggagaacc tgctccagag catcctcggc
       61 gtcatcacga tcttcatggt cagccagctt ggctcgtccg aggtggccgc cgtcggtgcc
      121 tcccagcagc tgcagatgct gttcatctcg gccttcttcg cgctctcgat gggcgcgacc
      181 gtcatcgtcg cccacgccta cggggcgcga cagcacgacg cgatcgggat ggcggccaag
      241 caggcgactg tcgcgacgat cgttctctcc agcatcatcg cggtgatcgt cttcgcgctg
      301 gccgagccaa tgctgcgctg gatgggcgcc gacagcgacg tgatcgccca ggggacccgc
      361 ttccagcaga tctcggccgt cggctacgtc ttcatggcgc tgatgttcgt cctcggcggc
      421 gcgctacggg gcgtcggcga cacgcgaacg ccgatgctgg tgacgctcgg catcaacgtc
      481 atcaacgtcg ccgtcgcctg gccgctgatc ttcggcaagc tgggaatgcc acagctcggg
      541 ctggacggcg cggcgatcgc ccagaacatc tcccgcttcg ccggcttcgt cgtcctggcc
      601 tggcttctct atcgcggcaa tcgcggtgtc agcatcgccg gccgggacgg ctggcggccg
      661 aacgtgacgt tcctcaagcg gctggccgat atcagcattc cggcgatggc cgagtcgctg
      721 ctgcgcggtg gcgggcagat tctgttcgtc gtcgtcgtct tcatgctcgg gacagcggtt
      781 gtcgccggcc acaacatcgc ccagcaggcg gttttcctct cgatgttccc cggcttcggc
      841 ttcgcgatgg cggcgacggc actgaccggc cagtcactcg gggccgggaa tccggcacgg
      901 gcacgggcgg cgacgttgac ggcgacccgc gcctgcatcg tctggatgtc gctgatgggt
      961 gtcctcttct tcgtcttcgc cgagccgatc atggagttct cggcgccgga cgaggccgga
     1021 cgacagcaga tcatcgactc cggcgtcgca gcgttacgcg ttatcgcctt cgcccagccc
     1081 ttccaggcga tcggcttcgt gctggcgggc gcgctgcgcg gtggcggtga cacgcgcttc
     1141 ccgatgttct cgaccggcat ctcgatgtgg atcttccgca tcccgctggc gtacctgttc
     1201 gcgatcacgg ccgacctcgg cctggccggc gtctacctgg cgatggggac cgacaacgcg
     1261 atcctgatgg cgctcaacat ctggcgctac cggcagggca agtgggcgga gaaccgcatc
     1321 accgtcaccg aggagccgga ccattccgcc gaaccgacca ccccaccgcc gacgctcctc
     1381 cccgccccgg agcctgatcc tgtggccgca ggcggcgacg ccgccgagtg aacgatgtcg
     1441 atgcactcat ccgcgcgctg attcacggtg agcgccaacc agcggacgac gacgcgacca
     1501 ccatcctcga acacatcgcg ggatcaacgt tctatccggg ccgacgcaac atcccccgcc
     1561 cgctgcagga gctgatgatc aggcacggct acgaggtgcc cgatcgaggc gacgatctca
     1621 tctatcactg ggcaaagcat gtccttgcgg atcagcagtg gacagtagac acaacggcaa
     1681 cggcgtattt tcagttcgcc gtgcgtgccg tgacacatcc ggccagccgg ttgcttgttc
     1741 agttcgcacc atctggtcac cgagttgcag tggtcatcag cgccgtcgat gatatcgtcc
     1801 cactcgcatc acaaggggcg cgctcaggtc gcaatctgct cgtcgtctat tctgccgatg
     1861 ttggtaggat agtttcggcg cacatgacgg catcgacatc acggattacg tcgaggacag
     1921 gaacagtatg gctacgcagg taacctccaa gatccaggaa gatattgact ggtacatggc
     1981 tgccctcgaa cgagaatgga aatcggccgt cgcgttagtt gctaactggg acagcgccga
     2041 tcccttcgac aaagatgatc tcgcgatcga gtggcatttg accatcgact ttctcctgcg
     2101 cgtcttgaaa taccaggaat gcggcctcat gtctgccgaa cagcagcagc gctttacgga
     2161 gattcgtcag ttcatgcgcg atcacgaagc ggagatcgac gctgtgctcg gtcccggtga
     2221 ggcgatggtc gatccgcctc gggagtggta tgtctcgctc gaacaataac ctcaacctcc
     2281 agtgccgatg atgagccgga tcgagcagcc gccgttcgcg ctgcggctgc tgatgctgat
     2341 tggcgggttg ttcgtcttcg cgctggcgat ggcgttgtcg ttgcagtgca acctcggcgc
     2401 ggcctcgtgg accgtcttcc acgacggcat cagccgacag acgccgctct cgattgggat
     2461 ggcgacgcaa ctggtcgggc tgctgatgct gggcgtctcc tggctggcag gcatccggcc
     2521 cggctttggg accgtcgcca acatgattct ggtcgggctg tttctggacc tgatcctctg
     2581 gaccggcgtg attccggagg cggacgccta cccgttgcgg gtcgcgatgc tgctggccgg
     2641 gatcgtcgtc ctcgggctgg cgtcggcgct gtatatcaag gccggcttcg gagccgggcc
     2701 gcgcgattcg ttcatgctgg cggtccatca gcgaaccggc ctgcggatcg gcaaggtccg
     2761 ttggatgatg gagtcgggtg ccgtcgtcgc cggtatcatc ctcggtggcg cgttcggcgt
     2821 tgggacgatc atcttcgctt gcctggtcgg cttctcggtc gatttcttct tcaatctgct
     2881 gggagtgcgc acccggccga agcctgaggc ggccagcccg acgccgatgg cggccgactg
     2941 atggtggcag gagcgggccg gtgcttgcca tactacgggg tgtggacgtg cgaaacgatg
     3001 ggcgaggggt gaagtggcga tgagcgggag cgatcaggga cagcatagcg acccgagtgc
     3061 cggaaccgga accggcgagg tcaatcttga gactggcggc ggcgcggccg tcgaggtgga
     3121 agaggccagc gcacctaccg cactcagcac tgagacgccc gtcgcgcggc tggaggcgat
     3181 gcgagcgcgg gtgatgccga tcctgcagtc gatcaagacc gagtacacgg gccggacccg
     3241 caacggctat ccaacgatcg tcgacaacat ccagcccggc ggtgtcttcg gcctcaacct
     3301 cgatcccggc tgggccatct acttcatgac cgacggcaca tcgctgtttg ccgagttgca
     3361 caagacgtcg cttcggaccg acacgctctc gatggcaaac gtcgagaagt tcgccggccg
     3421 gccctcgatc gaccggcgcg acatcgacga gacctgg
//
LOCUS       DASUGJ010000085         5945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_289083, whole genome shotgun sequence.
ACCESSION   DASUGJ010000085 DASUGJ010000000
VERSION     DASUGJ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289083"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..986
                     /gene="panC"
                     /locus_tag="VFV93_01900"
     CDS             132..986
                     /gene="panC"
                     /locus_tag="VFV93_01900"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5164122.1"
                     /translation="MRICTTIEEAREVRAALSDQLGFVPTMGALHAGHRELIRRARAE
                     NSAVAASIFVNPTQFTSSEDFQHYPRTLDTDLEILEAAGVDLALVPDASALYPAGFST
                     TIDVGPITRVFEGAARPGHFQGVATVVAILFNIIAPARAYFGQKDAQQVIVVRTLVND
                     LQLPIEIVSVPTVRDRDGLALSSRNARLTPTQRRAAVSIPRALDAVQQAWAAGERDSS
                     ALNARARAELSSQRDITVEYLKLTDPEMFAAWSGTIDGDALVLIAATLGDVRLIDNCL
                     LETSRPAT"
     gene            1040..3031
                     /locus_tag="VFV93_01905"
     CDS             1040..3031
                     /locus_tag="VFV93_01905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5164123.1"
                     /translation="MATTDRRPLVAEDVTLFNIIDDAQISPDGSTVAYSVRRSILDEN
                     RYASAIYTVSTDGGEPRQLTTGTGRDGSPRWSPDGRTLAFVSDRAGAPQIYLLDLGGG
                     EARQLTTLGRGAGSPVWSPDGSKIAFLSSEGYGIDDETRNKPGGFIRHIKRLDYRFNE
                     ADYIDDRFSQVWIVDIDGGEPRRLTAGDTSVASFAWSPDGETIAFSVNRIDRSAFYSQ
                     LYVLPVSLAGSTGDEDGALRISNGTDVAANPAWSPDGQRIAFIGRTSGTRAGGNGDIF
                     IVAPDGEEQRAITDGWDRSPATASYSDSWSPRDATPLAWTTDGSGVMFTASDKGQVGL
                     YKAGVHGDITLLNGGERSISIVSRSADGGRLAFVAASFTNPCDVYTCRADGSDERRLT
                     QVNAELLDEVIIQTPEHMPFESFDGAFQLDAWIIRPVGVEAGTKAPLVQHIHGGPHSI
                     FGHTFFFDMQLWASQGWNVLFMNPRASQGYGEAFASGAIADWGGVDWREQEQALDLAI
                     ERGGVDPDRLAVTGLSYGGFMTNWIVGQTNRYKVAASENGICNLVSFYGTSDIGWYFL
                     EHEMEKPIWENLDWYMRASPIYYIEQMQTPMLLLQAEADYRCPIEQGEQLFTALLARG
                     VPTEMVRFPGESHGQLAIGKPETRLVRRQVTLEWFKRFL"
     gene            complement(3068..3754)
                     /locus_tag="VFV93_01910"
     CDS             complement(3068..3754)
                     /locus_tag="VFV93_01910"
                     /inference="COORDINATES: protein motif:HMM:NF039470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HEX5164124.1"
                     /translation="MSLVQRDYLLRIVEQFVEVLNHVLHLRRGGSLLEARNYIEQHMG
                     RLVGIDPDDVAAQSAVELAAEIRGRLLRHQQPQLVSDQLVVLAGLLHESAHISDEAGD
                     QPRAMAERLKALDLYIEVLTVDEPGCAPAEAAVDVILDELPDLPAPATMRKLVDAYEA
                     SGRFDEAEDWLFHLIDAASDDNAVLAMGIAFYERLLALDDSTLAAGRLPADEALAGLA
                     ELLERQATNE"
     gene            complement(3842..4948)
                     /locus_tag="VFV93_01915"
     CDS             complement(3842..4948)
                     /locus_tag="VFV93_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5164125.1"
                     /translation="MLSNQRAAYDAMRERCPVAHSDFLGWSLFRHQDIVDVLDDPETF
                     SSASRHRAIPNGIDPPEHTVYRRLLEPYFSSESIMRFEPALRKIATEMTQALLDGNSD
                     TVDLISEFAEPFPLRALCVFLGWRDDNWERLRGWNHGNQQVALSSDRAAGKILAAEFT
                     SYVEQELQAHRAAGQDQLHDITTDLMTREVNGARLSDEDIVSILRNWTAGQGTVASGI
                     GNVIHYLAQDGQLQAQLRANPGLLPAAIDEILRVDGPLVMNRRTATRDVQIGGRQIGT
                     GERLTLIWIAANRDERAFDDPESVRLDRDARNNLVFGMGIHDCVGAPLARLELRLAVE
                     ELLGRTSQIELAEDDPPRAVYPSNGFRSLMVRLR"
     gene            5214..5567
                     /locus_tag="VFV93_01920"
     CDS             5214..5567
                     /locus_tag="VFV93_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5164126.1"
                     /translation="MTTIYGPVQLDDEIERFQPGERDSVSGRRSETLIKTDDLRVVLI
                     TMQAGASLAEHTAPGTITIQVVQGEMTVEVGEASHELAAGGLISLAGGVRHAVHARSD
                     GAFLLTIAWPPRSGR"
     gene            5573..>5945
                     /gene="eutB"
                     /locus_tag="VFV93_01925"
     CDS             5573..>5945
                     /gene="eutB"
                     /locus_tag="VFV93_01925"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF018461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEX5164127.1"
                     /translation="MTYRSSLAHRTYQFRDLRDLLAKATPLRSGDVLAGLAAADAEER
                     VAAQLALADLPLTTFLNECLVPYEDDEVTRLIIDSHDSATFATISQLTVGGFRDWLLS
                     DAATSEVLAALAPGITPEMAAA"
BASE COUNT         1111 a         1952 c         1876 g         1006 t
ORIGIN      
        1 tcttcgaggc tgagatcgcc gacagcttcg gtccaatcag ccacgcgcgc gtgaacatca
       61 tcccggacgg cggcgtcagc aggattcggc tgttcggcga accggagcag cgcgggtaag
      121 ggctgccaac gatgcgcatc tgcacgacga tcgaagaagc gcgtgaagtg cgagcggcac
      181 tgtccgatca gttggggttc gttccgacga tgggcgcgct gcacgccgga catcgggaac
      241 tgattcggcg tgcgcgggcc gagaatagcg ctgtggcagc ctcgatattc gtcaacccaa
      301 cccagttcac atccagcgag gattttcagc actacccacg gacgctcgac accgatctgg
      361 aaatcctcga agcggccggc gtcgatctgg ccctcgtccc ggacgccagc gcgctctatc
      421 cggcaggctt ctcgacaacg atcgacgttg ggccgatcac gcgggtcttc gaaggcgccg
      481 cccggccggg ccatttccag ggcgtcgcaa ccgtcgttgc gatcctcttc aacatcatcg
      541 cgccggcacg ggcctacttc ggccagaagg acgcccaaca ggtcatagtc gtccgcacac
      601 tggtcaacga cctccagcta ccgatcgaga tcgtcagcgt tccaaccgtc cgtgatcgcg
      661 acgggctcgc gctcagcagc cgtaatgcgc gcctgacgcc gacacaacgg cgggcagccg
      721 tatcgattcc acgtgcgctc gacgccgttc agcaggcatg ggcggctggt gagcgtgatt
      781 cgagcgcgct aaatgcacgg gcgagggccg aactcagcag tcagcgggac ataaccgttg
      841 aatatctgaa gctgaccgat ccggagatgt ttgcggcatg gtccggaacg atcgacggcg
      901 acgcgctggt gctgatcgcg gcaacgctcg gagatgtccg tctgatcgac aactgcctgc
      961 tggagacttc acgaccggcg acgtagacgc taggatggcg ccggcatgcg agacagccgt
     1021 agcaggggag gaaacgacga tggcaacgac ggaccggcga ccactggtcg ccgaagacgt
     1081 gacgctgttc aacatcatcg acgacgcgca gatttcgccg gatggatcga cagtcgccta
     1141 cagcgtccgg cgctcgatcc tcgatgagaa ccgctacgca tcggcgatct acacggtttc
     1201 aaccgacggc ggcgagccgc gccagttgac gaccggcacg ggccgcgatg gatctccgcg
     1261 ctggtcgccg gatggacgca cgctggcgtt cgtctccgac cgggctgggg cgccgcagat
     1321 ctacctgctc gacctcggcg gaggcgaggc ccggcagctg acgacgctgg gacgtggagc
     1381 aggcagcccg gtctggtcac cggatgggtc gaagatcgcg ttcctctcca gcgagggcta
     1441 cggcatcgac gacgagacgc gcaacaagcc gggcggcttc atccgccaca tcaagcggct
     1501 cgactaccgc ttcaacgagg ccgactacat cgatgaccgc ttcagccagg tctggatcgt
     1561 cgatatcgac ggcggcgagc cgcgacgact gacggccggc gacaccagcg tcgcctcgtt
     1621 cgcctggtcg ccggacggcg agacgatcgc cttcagcgtc aaccggatcg accgttccgc
     1681 tttctactcg caactgtatg tgctgccggt cagcctggcc gggagcaccg gcgacgagga
     1741 cggagcgctg cggatcagca atggcacgga cgtcgccgcc aacccggcct ggtcgccgga
     1801 tggccagcga atcgccttca tcggccgcac gtcgggcacg cgggcgggag gcaatggcga
     1861 catcttcatc gtcgcgccgg atggcgagga gcagcgggcg atcacggatg gctgggaccg
     1921 cagcccggcg acggcatcct acagcgacag ctggagcccg cgcgacgcga ccccgctggc
     1981 ctggacgacc gacggcagcg gcgtcatgtt cacggccagc gataaggggc aggtcggcct
     2041 ctacaaggcc ggcgtccatg gtgacataac gctgttgaat ggtggcgagc gctcgatctc
     2101 gatcgtctcg cgctcggccg atgggggacg gctggccttc gtcgccgcca gcttcaccaa
     2161 cccgtgcgat gtctacacct gccgcgccga tggctccgac gaacgacggc tgacgcaggt
     2221 caacgccgag ttgctggatg aggtgatcat tcagacgccg gaacacatgc cgttcgagag
     2281 tttcgacggc gcattccagc tcgacgcctg gatcatccgt ccggtcggcg tcgaggccgg
     2341 aacgaaggcg ccactggtcc agcacatcca cggcgggccg cattcgatct tcggccacac
     2401 cttcttcttc gatatgcaac tgtgggccag tcagggctgg aacgtgctgt tcatgaaccc
     2461 gcgcgccagc cagggctacg gcgaagcctt cgcgagcggc gcgatcgctg actggggcgg
     2521 tgtcgactgg cgcgagcagg agcaggcgct ggatctcgcg atcgaacggg gcggcgtcga
     2581 tccggatcgg ctggccgtca ccggcctctc gtacggcggc ttcatgacca actggatcgt
     2641 cggccagacc aaccgttaca aggtcgccgc cagcgagaac ggcatctgca acctggtctc
     2701 gttctacggg acgtccgaca tcggctggta cttcctggag cacgagatgg agaagcccat
     2761 ctgggagaac ctggactggt acatgcgtgc atcgccgatc tactacatcg agcagatgca
     2821 gacaccgatg ctgctgttgc aggccgaggc cgactaccgc tgcccgatcg agcagggcga
     2881 gcaactgttc acggcgctgc tcgcgcgcgg cgtaccgacc gagatggtcc gcttccccgg
     2941 tgagagccac ggccagctcg cgatcggtaa gccggaaacc cggctggtgc gccgccaggt
     3001 cacgctcgag tggttcaagc gcttcctgta agtggtcgcg cccggtattg ggatcgatgc
     3061 cgggcgctta ttcgttcgtc gcctgccgct ccagcaactc agccagacca gccagcgcct
     3121 catccgccgg cagacgacca gcagcgagcg tcgaatcatc cagcgcgagt agccgttcgt
     3181 aaaaggcgat acccattgca aggaccgcgt tatcatctga cgcggcatcg atgagatgaa
     3241 acagccagtc ctcggcctca tcgaaccggc cggatgcctc gtaggcatcg accagcttgc
     3301 gcatcgtcgc cggagcgggg aggtcaggca gttcatcgag gatcacatcg acggccgcct
     3361 cagccggcgc acaacccggc tcatcgacgg tcagcacctc gatatagaga tcaagcgcct
     3421 tgagccgttc tgccatagcg cgcggctgat cgccggcctc gtcggagatg tgcgccgatt
     3481 cgtgcagcaa tccggccagg acgaccagct ggtcgctgac cagttgcggt tgctggtggc
     3541 gtaacaaccg gccgcgaatc tcggccgcaa gctcgacagc actctgggcg gcgacatcgt
     3601 ccggatcaat accaaccagt cgccccatgt gctgctcgat atagttgcgc gcctccagca
     3661 gcgatccacc gcgccgcagg tgcaggacat ggttcagtac ctcaacgaac tgctcgacaa
     3721 tccgcagcag gtaatcgcgc tgaacgaggc tcatctgtgt tcctgtccac ccgtgacgcg
     3781 ccgaacggtt ccgttgatgc cgatgctaac cgatctcgag catgccggtt ccaggcgacg
     3841 atcagcgaag gcgcaccatc agcgaacgga agccgttact ggggtagacg gcgcgaggtg
     3901 gatcgtcttc cgcaagctcg atctggctgg tccgcccgag cagttcctcg accgcgagac
     3961 gaagctcgag ccgcgccaac ggtgcgccga cgcagtcgtg gattcccatt ccgaacacca
     4021 gattattgcg agcatcacga tcaagccgca ccgattcagg atcgtcaaag gcgcgttcat
     4081 cgcgattagc cgctatccag atcagcgtca ggcgctcgcc ggttccgatc tgtcgcccac
     4141 caatctggac atcacgagtg gcagttcgtc ggttcatcac caacgggcca tccacgcgca
     4201 gaatctcgtc aatagcggcc ggcaacaggc cagggttcgc ccgaagctgc gcctgcaact
     4261 gcccgtcctg cgccagataa tggatgacgt tcccaattcc gctggcgacc gtcccttgtc
     4321 ctgcggtcca gttacgcagg atgctgacga tatcctcgtc gctcagccgc gccccgttga
     4381 cttcgcgcgt catcagatcg gtggtgatgt cgtgcaattg gtcctggccg gccgcacgat
     4441 gcgcctgaag ttcctgctcg acgtaactgg tgaactcggc ggccagaatc ttcccggcag
     4501 cgcgatcgct gctgagcgcc acctgctggt taccgtggtt ccagcctcgc agccgttccc
     4561 agttatcatc ccgccatcca agaaagacgc acaacgcgcg taacggaaac ggctcggcaa
     4621 actcggagat cagatcaaca gtgtcactgt tcccatcaag caacgcctgc gtcatctctg
     4681 tggcgatctt ccggagagca ggctcaaacc gcattatcga ttcgctgctg aagtacggct
     4741 ccagcaaccg gcgatagacg gtgtgttccg gcgggtcgat gccattcgga atcgcacggt
     4801 ggcgcgacgc gctgctgaag gtttcgggat cgtccaggac atcgacaatg tcctgatggc
     4861 ggaacagcga ccagcccagg aagtcgctgt gcgcgaccgg gcagcgttcg cgcatggcgt
     4921 catacgccgc gcgctggttg ctcagcacgt gctcgtcaat tggattccag tctgccgatg
     4981 gcctgttact caattcctca ctccaaaccg tctagccatt ccgcacatac tgaccatacc
     5041 accgcgtcgg cagcgtaacc gcaatcttgg ccggatcaca gcctgtacgc cagacgcggc
     5101 tatgtccgcg ccaccacagt ggcttaccgt cgtcaatccg ctgacaacac gacgcggttg
     5161 ccactgtatg attccagcgg ggagtaccgc accgtccagg gaaggaagag cgcatgacga
     5221 cgatttatgg cccggtccag cttgacgacg agatcgaacg gttccagccg ggcgagcgtg
     5281 attccgtctc agggcgacgg tcggagacgt tgatcaagac cgacgacctg cgcgtcgtcc
     5341 tgatcacgat gcaagcgggc gccagcctgg ccgagcacac cgcgccgggt acgatcacga
     5401 tccaggtcgt gcagggggag atgacggtcg aggtcgggga ggcgtcccat gaactcgcgg
     5461 ccggtgggtt gatctcgctg gctggtgggg ttcgtcacgc cgtccacgcc cgctccgacg
     5521 gcgccttcct gctgacgatc gcctggccgc cacgctccgg ccgctgaccc agatgaccta
     5581 ccgctccagc ctggcacacc ggacgtatca atttcgcgac ctgcgtgacc tgctggcgaa
     5641 agcgacaccg ttacgctctg gcgatgtcct ggccgggctg gccgccgccg acgccgagga
     5701 acgggtcgcc gcccagctgg cgttggcaga cctgccgctg acgaccttcc tgaacgagtg
     5761 cctggtgccg tacgaggacg acgaggtcac ccggctcatc atcgacagcc acgacagcgc
     5821 cactttcgcg accatcagcc agttgacggt cggcggcttc cgcgactggc tgctgtctga
     5881 cgcggcaacc agtgaggtgc tggcggcact ggcgcccggc atcacgccgg agatggctgc
     5941 cgccg
//
LOCUS       DASUGJ010000086         4519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1261720, whole genome shotgun sequence.
ACCESSION   DASUGJ010000086 DASUGJ010000000
VERSION     DASUGJ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261720"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFV93_01930"
     CDS             complement(<1..266)
                     /locus_tag="VFV93_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5164128.1"
                     /translation="MTDTLRVAVLGAGRWAQTAHIPGWQRDPRCEIVVICDPAEDRAR
                     DFAHEFGIPAHSRDWQAVVGRDDIDIVDVATPSRTHFELAWAAL"
     gene            complement(298..1377)
                     /locus_tag="VFV93_01935"
     CDS             complement(298..1377)
                     /locus_tag="VFV93_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX5164129.1"
                     /translation="MRAAVCYEFGQPLVIEEIEIDPPQQGEVRVRVTACGICHSDLHR
                     LRGERGGSLPIVAGHEVAGIVEELGPGVSGIAPGDRVIVSLLRSCGRCFFCQTGAPHH
                     CEENFALQHETRLHTRDGVPIVQGVNVGGFAEQVVVDQSQLVTVPDDVPLESVALLAC
                     GVITGFGAVVNTANVPAGSSVVVIGTGGVGMNAIQGARLSGANPIIAVDVLDGKLEDA
                     RGFGATHGVNATRSDAAEAVRAISGARGADYVFVTVGSAQAAELGLRLVRDAGTLVLV
                     GMPPTGVDLPLPVFETVYRSVRILGSKMGGTRLSVDVPRLLDLYQAGRLQLDELITER
                     YPLERINEAVASVERGEALRHVIVM"
     gene            complement(1570..2010)
                     /locus_tag="VFV93_01940"
     CDS             complement(1570..2010)
                     /locus_tag="VFV93_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5164130.1"
                     /translation="MSIKRMDNVLIVVDDLEAAKAFFLDLGLELEGETTVEGPSVDRL
                     IGLQNVRATLTTLRTPDGHGRIELDKFHTPEAIRTGPEHAPVNALGIRRIMFAVDDLD
                     GLVARLLAGGAELVGDIVQYGDTFRLAYVRGPEGIIVGLAEELS"
     gene            2201..3097
                     /locus_tag="VFV93_01945"
     CDS             2201..3097
                     /locus_tag="VFV93_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164131.1"
                     /translation="MTMEPGVILERVKATAVPAEDPYLKEGLVRPNSFQRTYTGMMLA
                     AGMFGTLLPPIVVFGGVLLAREWAFKGSLSSYYHSGMRDVFVGMLCIVGFFLVVYKLF
                     EWKSDNIFSIGAGIGAATLALFPTRLGEGISKDELTPLQSRVGENLTFYIHIGATSLF
                     FTCLVIICFGFAIQERQRNQERKAGKSKYPPQFWFGFHLAAVGVMIGSLVFILTAWFV
                     ERQGWYDGFIITYATLFGESAAIFAFGLSWWTKGSEFPKLRAVDPATGEQVVVNPQVD
                     AAPSAALEAQPPVDVVLKPSDS"
     gene            complement(3206..4399)
                     /locus_tag="VFV93_01950"
     CDS             complement(3206..4399)
                     /locus_tag="VFV93_01950"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5164132.1"
                     /translation="MTLPLDGVRIIALSQFGAGPFGMMQLADLGAEIIKIEDPSTGGD
                     VARSVPPFREGQDSLYYQAFNRNNKSVTLNLRVPEARGVLHDLVAVSDGLFSNLRGDQ
                     PEKLGLTYPQLQHVNPRIVCCSLSGFGMTGPRRAEPGYDYLFQAYAGYMALTGDPATP
                     PAKSGISIIDMSGGLAAALGLMAGILQAQRTGRGSDVDVSLLDTAISMLTYLATFNLN
                     RGYQPERLPDSAHPTLYPSQVFQTADGYIVVTVMKEKFWPLLCAALERSDLTRQPHFV
                     DFDARFEHRDELLAILKDVFRTRTTAAWMDRLAGSVPCAPVNSIEQALDDPQVLARNM
                     IVETQNAAFGPMRHVAGAITFPGQPRQQHDAAPDLGAHTDQILRDELAYSPEQIARLR
                     ACGAI"
BASE COUNT          742 a         1462 c         1510 g          805 t
ORIGIN      
        1 tccagcgccg cccaggccag ctcgaagtgc gtccgcgagg gggtcgcgac gtcgacgatg
       61 tcgatgtcgt cccgcccgac gaccgcctgc cagtcgcggc tgtgcgcggg gatgccgaac
      121 tcatgggcga agtcgcgagc acggtcctcg gccggatcgc agatgacgac gatctcgcaa
      181 cgcgggtcgc gctgccagcc ggggatgtgc gccgtctgcg cccagcgacc ggcgccgagc
      241 accgcgacgc gcaatgtgtc agtcatggat gcaccttcct gccgtcacct tgccgcgcta
      301 catcacgatg acgtgacgca acgcctcgcc gcgttcgacc gacgcgaccg cctcgttgat
      361 gcgctccagc gggtagcgct cggtgatcag ctcgtccagt tgcagccggc cggcctgata
      421 caggtccagc agtcgtggga catcgaccga cagccgcgtc ccgcccatct tgctgccgag
      481 gatccgcacg ctgcgataga ccgtctcgaa gacgggcagc ggcaggtcaa cgccggtcgg
      541 cggcattccg accagcacca gcgtccccgc gtcgcgcacc agcctcaaac caagctcggc
      601 cgcctgggcg ctgccgaccg tgacgaacac atagtctgcg cctctcgcgc cgctgatcgc
      661 gcgcactgcc tcggcagcgt ccgagcgcgt cgcgttgacg ccatgcgtgg cgccaaagcc
      721 acgcgcgtcc tccagcttgc cgtcgaggac atcgaccgcg atgatcgggt ttgcccccga
      781 tagccgcgcg ccctggatcg cgttcatgcc gaccccgccg gtcccgatca cgaccacgct
      841 gctgccggcc gggacgttgg ccgtgttgac gactgcgccg aaaccggtga tgacgccgca
      901 ggccagcagc gccacgctct ccagcggcac gtcgtccgga accgtcacga gctgcgactg
      961 atcgacaacg acctgctcgg cgaagccacc gacgttgacg ccctggacga tcggcacacc
     1021 gtcgcgggtg tgcagccgcg tctcatgctg cagtgcgaag ttctcctcac agtgatgcgg
     1081 tgctccggtc tggcagaaga agcagcgccc gcacgagcgc agcagcgaga cgatgacacg
     1141 gtcgcccggc gcgataccgc tgacacccgg ccccagctcc tcgacgatcc cggccacctc
     1201 gtgtccggca acaatcggca gcgatccgcc gcgctcgcca cgcagccggt gcaggtcgct
     1261 gtggcagatg ccgcaggctg tcacccgcac gcgcacctcg ccctgctggg gcgggtcaat
     1321 ctcaatctcc tcgatgacca ggggttgccc gaactcgtag cagactgcag cacgcatcgt
     1381 tgcccctcca agctgctcgc ttgcgtacgg ttgctgtcgt cggtacggga catttgccgc
     1441 gctagagttg cgatacggct atcatggggc cacagggcgc cagcgtcaac cacacaacgc
     1501 cggggtcggg gatggcgtgg gatcacagca ctgaggacgc tagcagtttc cacgaaggcg
     1561 gtgagcgctc tagctgagtt cctcagccag cccaacgatg atgccctccg ggccgcggac
     1621 gtaggcgagc cgaaaggtat caccgtattg gacgatgtcg ccgaccagtt cggcgccacc
     1681 agcaagcagg cgcgcgacga ggccgtcgag atcgtcgacg gcgaacatga tgcgacggat
     1741 cccgagtgcg ttcaccgggg catgttcggg ccccgttctg atcgcctccg gcgtgtggaa
     1801 tttgtccagc tcgatccgcc cgtggccatc cggagtgcgc agcgtcgtga gggtggcgcg
     1861 gacgttctgc aggccaatca gacgatcaac ggaggggcct tcaacggtcg tctcgccctc
     1921 cagctcaagt ccgaggtcga gaaagaaggc cttcgcggct tcgaggtcgt cgacgacgat
     1981 gaggacgttg tccattctct tgatactcat cgctccagta tagcagcggc cgttggcggc
     2041 gaatcacgcc ggtcgtccac gcgaggtggt ggcgagacct tcggcgatga tgtgtcgcgg
     2101 cctgcttcgg cttgacatag cggaagaatg ggttcatgct ggattgcaca taacgcacgg
     2161 attccagacc ggtcttatcc tccaccgcca ggatcggggg ttgacgatgg aaccaggggt
     2221 tattctcgag cgcgtcaagg ccaccgctgt tccagccgaa gatccgtatc tcaaagaggg
     2281 tctcgtcagg ccaaattcgt tccagcggac gtacacgggg atgatgctgg cggcggggat
     2341 gttcggcacg ctgctgccgc cgatcgtcgt cttcggcgga gtcctgctgg cgcgcgagtg
     2401 ggcgttcaag gggtcgctca gttcgtacta ccactccggc atgcgcgatg tcttcgtcgg
     2461 catgctgtgt atcgtgggct tctttctcgt cgtctacaag ctgttcgagt ggaagtcgga
     2521 caacatcttc agcatcggcg ccggtatcgg tgcagcaacg ctcgcgttgt tcccaacgcg
     2581 gttgggcgag ggtatttcca aggatgagct gacaccgtta cagagccgcg tcggtgagaa
     2641 tctcaccttc tacatccaca tcggcgcgac cagcctcttc ttcacctgcc tggtgatcat
     2701 ctgcttcggg ttcgccatcc aggagcggca gcggaatcag gaacgcaagg cggggaagtc
     2761 gaagtacccg ccgcagttct ggttcggttt tcatctcgct gccgtcggcg tgatgatcgg
     2821 gtcgctggtg ttcattctca cggcctggtt tgtggagcgt cagggctggt acgacggctt
     2881 catcatcacg tacgcgacgc tgttcggcga atcggccgcc atcttcgcgt tcggcctgtc
     2941 gtggtggacc aaggggtcgg aattccccaa gctccgcgcc gtcgatccgg ccacgggcga
     3001 gcaggtagtg gtcaacccgc aagtcgacgc cgctccgtcg gccgcgctgg aggcgcagcc
     3061 gcctgtcgat gtcgttctca agccgagcga ctcctgatct cgttgctact gtttcgggtt
     3121 tgaatcagca caatcccaat gatcaatccg tattgcgacg gaagaacgaa cgacctgaac
     3181 ggtgtcctcc atcgaccagc gacgctcaga tggctccgca cgcgcgcagt cgggcgatct
     3241 gttcggggga gtacgcgagc tcgtcgcgca ggatctggtc ggtgtgggcg ccgaggtcgg
     3301 gtgcggcgtc gtgctgctgg cgaggttggc cggggaacgt gatcgcgccg gcgacatggc
     3361 gcatcgggcc gaaggcggcg ttctgggtct cgacgatcat gttgcgggcg aggacctggg
     3421 ggtcgtccag cgcctgctcg atcgagttca ccggcgcgca ggggacgctg ccggccagcc
     3481 gatccatcca ggcggcggtc gtccgggttc tgaagacgtc cttcaggatc gcgagcagtt
     3541 cgtcgcggtg ctcgaagcgg gcgtcgaagt cgacgaagtg gggctggcgc gtcaggtcgg
     3601 agcgctccag cgcggcgcag agcagcggcc agaacttctc cttcatgacc gtcacgacga
     3661 tgtagccgtc ggcggtctga aagacctgcg aggggtagag cgtcgggtgg gcggagtccg
     3721 gcagccgctc gggctggtag ccccggttca ggttgaaggt cgccaggtag gtgagcatcg
     3781 agatcgcggt gtcgagcagg ctgacatcga catcgctgcc ccggccggtg cgctgggcct
     3841 ggaggatgcc ggccatcagg ccgagcgcgg cggccagccc gccggacatg tcgatgatcg
     3901 agataccgct cttggccggt ggcgtcgccg ggtcgccggt cagcgccatg tagccggcgt
     3961 aggcctggaa caggtagtcg tagcccggct ccgcccggcg cggcccggtc atgccgaagc
     4021 cgctcaacga acagcagacg attcgggggt tgacgtgctg caactgcgga taggtcagcc
     4081 ccagcttctc gggctggtcg ccgcgcaggt tgctgaagag cccgtccgag acagccacga
     4141 gatcgtgcaa gacgccccgc gcttccggta cgcggaggtt gagcgtgacg ctcttgttgt
     4201 tgcggttgaa cgcctgatag tagaggctat cctgcccctc gcggaacggc ggcacggacc
     4261 gggcaacatc gccgccggtg ctcgggtcct cgatcttgat gatctcggcg ccgagatcgg
     4321 ccagttgcat catgccgaac ggccccgcgc cgaactgcga cagggcgatg atccgaacac
     4381 catcgagcgg cagggtcacg cgcgatctcc ctcgttgtct gttccggtcc gacgactcgt
     4441 gcgtcttccc catgtagggg ccgatcctgt atcggccagc gccgtgaggc gccttcgtcg
     4501 gacatgcatg gttgatcca
//
LOCUS       DASUGJ010000087         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1400554, whole genome shotgun sequence.
ACCESSION   DASUGJ010000087 DASUGJ010000000
VERSION     DASUGJ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400554"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFV93_01955"
     CDS             <1..327
                     /locus_tag="VFV93_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164133.1"
                     /translation="WILPLSNSLIVSIAGGLIAGLALGPFNIIATVALQERVPEAMLG
                     RVIGALSMVSLAAPLGVLLAGVGIDAIGIRPLMAIIASGISLIAVVIFIHPDLRKVER
                     LTVGDA"
     gene            407..1411
                     /locus_tag="VFV93_01960"
     CDS             407..1411
                     /locus_tag="VFV93_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5164134.1"
                     /translation="MHDPTQPAITATNLHRVFRAGSGPFRRGTEIVAVEDISFSVPQG
                     TIFGMLGPNGAGKTTTIKMLSTLLVPTSGSATVAGYDVRRDERQVRRRLGVVLGGDRG
                     LYMKISAIENLRYFGNLYGMRKPDIERRAAELLELVNLADRSSQRVEGFSRGMKQRLH
                     LAKALLHNPPILFLDEPTIGLDPASAVDLRRAVAALVPDHTVLLTTHDMHEADLLCRE
                     IAIVDHGRIVAQGTPEELKALVDEGRRVVITTLHPLNGSYGEVAARLSLIPNVRAVQQ
                     DLTSDGGGELTILCNDTANALDEALAIMRGAGASVNGVRVLEPTLEDAFLAIAGGRLQ
                     "
     gene            1408..>2138
                     /locus_tag="VFV93_01965"
     CDS             1408..>2138
                     /locus_tag="VFV93_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164135.1"
                     /translation="MSAVAQPHSELAGWWQVVAATCWTSFKRNSAYFINYIRTPMFPV
                     GMFLGSYFAWKASGATTIDGVNISGFLLAGMVGMICWYSSVWWTGSAIEEERWEGTIA
                     ALFLTPASRVAVVIGHGLGGFIYMIPSFATIVLLALITGARFNVGSLVAVVASALALL
                     VASLAMGFLLASLFILSRRANLMANIIQHPLYLMGGFMIPRDALPDWLHTISNAMPLA
                     HAVDAFRASMLASASLHDVGNSLLAA"
BASE COUNT          338 a          794 c          627 g          379 t
ORIGIN      
        1 tggattctgc cgttgtcgaa ttcgttgatc gtcagcatcg ctggcggcct gatcgccggc
       61 ctcgccctcg gacccttcaa cattatcgcg accgtcgccc tccaggagcg tgtcccggag
      121 gcgatgctcg gccgcgtcat cggcgcgctc tctatggtct cgctggccgc acctctcggc
      181 gtcctgttgg ccggcgtcgg tatcgacgca atcggcatcc gcccgctcat ggccatcatc
      241 gcctcaggta tctctctgat cgccgtcgtc atcttcatcc atcccgacct gcgcaaggtc
      301 gaacgactga cggtaggtga cgcgtgatgc gtgacgcgaa ggctggatcc agaccgcgtc
      361 acgcatcacg cgtcacgcgt cacgaacgaa gaaaggacct ccacccatgc acgacccaac
      421 ccaacccgcc atcactgcca cgaacctgca ccgcgtcttc cgggccggca gcggcccctt
      481 ccggcgcggg accgagatcg tcgctgtcga ggacatctcg ttcagcgtcc cgcagggaac
      541 catcttcggg atgctcggcc cgaacggcgc cggcaagacg acgacgatca agatgctctc
      601 aacgctgctc gtgccaacct ccggcagcgc caccgtcgcc ggctacgacg tgcgccgcga
      661 cgagcgccag gtccgccgcc ggctgggtgt cgtcctcggc ggcgaccgcg gcctctacat
      721 gaagatctcg gcaatcgaga acctgcgcta cttcggcaac ctctacggca tgcgcaagcc
      781 cgacatcgag cgccgcgccg ccgaactgct ggagctcgtc aacctcgccg accggtccag
      841 ccagcgcgtc gagggcttct cgcgcggcat gaagcagcgg ctgcatctcg ccaaggcgct
      901 gctccacaac ccgccgattc tcttcctcga cgagccgacg atcggcctcg atccggcctc
      961 cgccgtcgat ctgcgccggg cggtcgccgc cctcgtcccc gaccacaccg tcctgctcac
     1021 cacccacgac atgcacgagg ccgacctgct ctgtcgtgag atcgcgatcg tcgatcacgg
     1081 ccggatcgtc gcccagggca cgcccgagga gctgaaggcg ctcgtcgacg agggccgccg
     1141 tgtcgtcatc acgaccttgc acccgctcaa cggcagctat ggcgaagtcg ccgcccgcct
     1201 gagcctgatt ccgaacgtgc gcgccgtcca gcaggacctc accagcgacg gcggcggcga
     1261 actgacgata ctctgcaacg acaccgccaa cgcgctcgac gaggcactgg cgatcatgcg
     1321 tggcgccgga gcgtcggtga acggcgtccg cgtcctggag ccgaccctcg aagacgcctt
     1381 cctcgccatt gccggaggga gactacaatg agcgccgtcg cccaaccaca cagcgaactg
     1441 gccggctggt ggcaggtcgt cgcggccacc tgctggacct cgttcaagcg caactctgct
     1501 tactttatca actacattcg gacgccgatg ttcccggtcg gcatgttcct cggctcctac
     1561 ttcgcctgga aggcgtccgg cgccacgacc atcgacggtg tcaacatcag tggcttcctg
     1621 ctggccggaa tggtcggcat gatctgctgg tactcgtcgg tctggtggac cggatcggcc
     1681 atcgaagagg agcgttggga gggcacgatc gccgccctct tcctcacacc agccagccgc
     1741 gtcgccgtcg tcatcggcca cggcctgggc ggcttcatct acatgatccc ctcgttcgcg
     1801 acgatcgtcc tgctcgccct catcaccggc gcgcgtttca acgtcggctc actggtcgct
     1861 gtcgtcgcct cggcgctggc gctgctggtc gcctcgctgg cgatgggatt cctgctggcg
     1921 tcgctcttca tcctcagtcg ccgcgccaat ctgatggcga acatcatcca gcacccgctc
     1981 tatctgatgg gcggcttcat gattccgcgc gacgcgctgc ccgactggct acacaccatc
     2041 tcgaatgcga tgccactcgc gcacgccgtc gacgcgtttc gcgccagcat gctggcgtcc
     2101 gcgtcgctcc acgatgtcgg caactcgctg cttgcagc
//
LOCUS       DASUGJ010000088         6346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_428257, whole genome shotgun sequence.
ACCESSION   DASUGJ010000088 DASUGJ010000000
VERSION     DASUGJ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428257"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFV93_01970"
     CDS             complement(<1..459)
                     /locus_tag="VFV93_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164136.1"
                     /translation="MGYRLDLAVGTSYSLDLLALMTAPVAFALFDRQRGDGSLVDDPI
                     ATLQALREYATRISLFCQAGQISVPPEFRSLLVYLEGSIHPVIPPDPEAIFHPKVWYI
                     RYRGEGDGTTVYRLLCLSRNLTFDRSWDTILRLDGTAGQDSRWPELERFVN"
     gene            complement(503..1753)
                     /locus_tag="VFV93_01975"
     CDS             complement(503..1753)
                     /locus_tag="VFV93_01975"
                     /inference="COORDINATES: protein motif:HMM:NF039739.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6361 family protein"
                     /protein_id="HEX5164137.1"
                     /translation="MASSAFGWLDHDERERRRMMEVVNLFREKGTLDELGIGTIRDTI
                     ADRLFPGTSTIQTRARYFVFIPWIYVQIERDRVPSNRVDVHARKLQWQLVQSLKAGGE
                     GSNAGIIGIEAGENVQRLPSDIYWQGLGRWGIRLAAGSTERYQASIDAVHRHERQASM
                     SDGAELLTSARRSWDGSLPSPPSNFLEETTFALTRDEAEYLSERIQQSCPDSLLAGCM
                     RPSITRKRQSRTPWDLNGLESLSTDLQQDIADARRYSLVMEGAVLLYNLMLAERAVEW
                     AIASDSSRIEHYSNELHRWAGEMAGTQDDLEMWDRSRFWNRMYDRNPRLHPGSRQFSE
                     SWLQLAIVTRGSIREDDAARALIRARERRLKSALARLHNPRALERWSGASGVGQMTYR
                     WGNAQRILRDILDGLDRPAGATNA"
     gene            complement(1918..2658)
                     /locus_tag="VFV93_01980"
     CDS             complement(1918..2658)
                     /locus_tag="VFV93_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5164138.1"
                     /translation="MATFVLVHGGWHGAWCWQRVVPLLTAVGHAVETPTLAGLGERAA
                     ELRPEIDLEQHVAEVTASIDLSGPRDVVLVGHSYSGMIVAAVAARRAARIATLVYLDA
                     FVPEDGQSMFDIAPPGRPDGFRELARLHGDGWRVPIPTAQAMGIVDDAEARWLTERLT
                     PQSIATLEQPVRFGAAEPDPARVPRHFIHCLTDPPSITFLPFAVRARTTPGWTLHELT
                     TGHDAMLTAPDELAQLLLGMAPATTSEA"
     gene            2906..5884
                     /locus_tag="VFV93_01985"
     CDS             2906..5884
                     /locus_tag="VFV93_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HEX5164139.1"
                     /translation="MLVGQAASEPGRSIDASGRDGARSRIVGREREIGALRDQLRDVL
                     DGHGRLVLISGEAGIGKTTLVANLADVAVAHGCLVLWGHAYDLSVTPPYGPWLELLRR
                     YPAATNGLPLLPAVVEDGRSLATVGSQESLFATVATFFRAVANQTPLVLVLDDLHWAD
                     QASLDFLRYLARQISHDRILLLATYRSDEIHRHHPLFDLLPLLVRESGAERLEVHRLD
                     AAGHRALVANRYTLPQVDTDRLVQYLELHAEGNPLFALELLRTLEDEGNLRQTDAGWS
                     LGDAAQVRVPPLLRQVIDRRLARLGDNARSLLQVAAIIGQEVPLDLWQQIVEASDDAL
                     IDAIEQGQVTNLLDEVAGGASYRFQHALLREALYEGVVSLRRRSWHRKVAEALATQPN
                     PVPDVVAYHFQQAGDVRAVAWLLESGRRARMAYATASAIDRFETALALDEQHDGASGL
                     RGWLLAGLATCGALYSNTDERLRTLNEAMDFAAQTNDDVLMGLCEWSSTFLATSGPGS
                     ALQLYKARDRIQALPPEDRGRLYGFIYGSTGVALDPSGPDMTCEIVGLQGQSGQVRET
                     LAAVERIREEYPRLSATAELGVDNALMTSFQALGRPDDSLRHYEHMQAVHRRNHVTDW
                     IAITTWLKLRDLVLVYWPDRIATRLEVASDSIEAIRLAKAEGTLGPDVPDETGAIWLY
                     LVDGRWDDARRVVEVATSAGQWITFLTAAAWMTLTRHQGDPEAALARLPITFPDGPAS
                     QPGRQTFETSVLCMHAAIEIALDAGDLALARAWLECHDRWLDWSGHIPFTTIGHRLWA
                     RYHHVSGDRAAAIERANQALELASDPRQPLTLLGAHRLLGELETDGGNHGNARQHLAE
                     ALALADACQAPFERALTLVALAELALASSDIGGASPHLADARAICEPLGARPTLERIG
                     ALEAQLAAAPRQRHPAGLSAREVEVLRLVAEGLTDAEVAERLFLSRRTVSTHLTSIYT
                     KLGVNSRTAATRFAVEHGIT"
     gene            6055..6264
                     /locus_tag="VFV93_01990"
     CDS             6055..6264
                     /locus_tag="VFV93_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164140.1"
                     /translation="MMNPTDLDVRFLNHTANVSYADRFGSLYPATQPQQHSPRRYVAS
                     VLLRLSARLAPQQTQQAIARDSAGV"
BASE COUNT         1158 a         2090 c         1975 g         1123 t
ORIGIN      
        1 gtttacgaac cgttcgagtt ccggccaacg agagtcctga ccggcggtgc catcgagccg
       61 gagaatggtg tcccacgatc gatcgaacgt caggttacga ctcaggcaca gcagcctata
      121 gacagtcgtc ccatcaccct cgccccggta acggatgtac cagaccttcg gatgaaaaat
      181 cgcctcggga tcaggtggaa tcacggggtg gattgaacct tcgagataca cgaggagtga
      241 ccgaaactcg ggagggaccg agatttgtcc tgcctggcag aacagagata ttcgcgtcgc
      301 gtattcgcgc agtgcctgga gcgtcgcgat cggatcgtca actagtgagc cgtcgccccg
      361 ttgtcgatcg aacagggcaa aagcgaccgg tgcggtcatc agcgccaaca ggtcgagcga
      421 atagctcgtt ccaacagcca ggtccaggcg atatcccaac ggagggcgaa gtgcatcgag
      481 gagcaggtat cgcttctccg gatcaagcat tagtggcccc cgccggacga tcgagaccgt
      541 ccagaatgtc gcgcaggata cgctgtgcat tgccccatcg gtatgtcatc tgaccaacac
      601 ctgacgcgcc agaccaccgc tcaagcgctc tcggattgtg caaacgtgcc agtgcagact
      661 tcagccgtcg ttcacgtgcc cgaatgagtg cccgggcggc gtcatcttct cgaatagagc
      721 cccgagtaac aatcgcgagt tggagccaac tttcgctgaa ctggcgtgaa cctggatgca
      781 gtcgtgggtt ccggtcgtac attcgattcc aaaacctcga ccggtcccac atttccaggt
      841 catcctgagt tccggccatc tcgccagccc atcggtggag ctcattgctg tagtgctcga
      901 ttcgggacga gtcggacgcg atcgcccatt cgacggctcg ctctgccagc atgaggttat
      961 agagcagcac ggctccttcc ataaccagcg agtatcgtct tgcgtcggcg atatcctgtt
     1021 gcaggtcagt gctgagcgac tccaggccat tcagatccca cggtgtacgg gattgtcgct
     1081 tacgtgtgat ggagggtcgc atgcaccctg ccaacaggga gtcgggacac gactgctgga
     1141 ttcgttctga cagatactcg gcctcgtcgc gagtcaacgc gaatgtggtc tcttcaagga
     1201 agttcgatgg tggcgacgga agcgagccat cccaggagcg acgcgcggaa gtgagcagtt
     1261 cggccccgtc ggacatgctg gcttggcgct cgtgtcgatg tacggcatca atcgaagcct
     1321 gataacgctc ggtagacccc gcggcaagtc ggattcccca gcgaccgagt ccttgccaat
     1381 aaatatcact tggcaagcgc tgcacgttct caccagcctc aatcccgatg atgccggcgt
     1441 tagaaccttc accacctgcc ttcaggctct gaacgagttg ccactgaagc ttgcgggcat
     1501 gcacgtccac gcgattcgat ggaacgcggt ctcgttcgat ctgcacatag atccatggga
     1561 taaagacgaa gtagcgagca cgtgtctgga tcgtgctggt tccggggaac agacgatcgg
     1621 caatcgtgtc acggatcgtg ccaatgccga gttcgtcgag tgttcctttc tccctgaaca
     1681 gattcaccac ttccatcatc cgtcggcgct cgcgctcatc gtggtcgagc catccgaagg
     1741 ccgatgaagc catttcacac ctttcgcaat gggcatttca gaagcagtca tttcagaagc
     1801 agttagggcg cacatcattt gctggtcgtc gtcagcgatg atagcccatc tacgaatgtg
     1861 atcgcgatga agcccgcccg caacgagagc acgccggcac gtgcagacga aatggtctca
     1921 tgcctcactg gtggttgccg gagccatgcc gagcagcagt tgcgccagct catccggggc
     1981 ggtgagcatc gcgtcgtggc cagtcgtcag ctcgtgcagg gtccagcctg gagtggtccg
     2041 tgcccggacg gcgaagggaa ggaacgtgat cgacggcggg tcggtcaggc agtggatgaa
     2101 atgccgtggc acgcgtgccg ggtccggctc cgcagcgccg aagcgcaccg gctgttcgag
     2161 cgtcgcaatc gattgcggcg tcaggcgttc cgtcagccag cgcgcctcag cgtcgtcaac
     2221 gatccccatc gcctgtgccg tcgggatcgg cacccgccag ccgtcgccgt gcagccgtgc
     2281 cagctcgcgg aagccatcag gccgcccggg cggcgctatg tcgaacatcg actgaccgtc
     2341 ttccggcacg aaggcgtcga gatagacgag cgtcgcgatt cgcgccgccc ggcgggcagc
     2401 caccgccgcg acgatcatgc cactgtagct gtgaccgacg aggaccacgt cacgcggacc
     2461 gctcagatcg atcgacgccg tcacctcagc gacgtgctgc tcaaggtcga tctctggccg
     2521 gagctccgcc gcccgctcgc ccagcccggc gagggtcggc gtttcgaccg catggccgac
     2581 ggccgtcagg aggggaacga cgcgctgcca gcaccacgcg ccgtgccagc caccatgaac
     2641 gaggacaaag gtcgccatct gtgaacgccc cttcgttcgg ttatccagcg gtggatcgac
     2701 gcgcttgcgt tatcagttgc cgggggaaac atctggtgaa cggggagcac cgatggcgtc
     2761 ccaaactgag acccctgctc ggccgccacc gccggtggac ggtagacaac agcgatcgcc
     2821 gcatcgatcc tcccaacgct ttcggtgtag ctccggttgc tttacactag cgaccctcgt
     2881 gcggtttgtc tacagttgga gaggcgtgtt ggtgggacag gcggccagcg agccgggacg
     2941 gtcgatcgac gccagtggtc gcgacggtgc gcgatctcga atcgtcggtc gtgaacgcga
     3001 gatcggcgca ctgcgtgacc agctacgcga cgtcctcgat ggacatggtc gcctcgtatt
     3061 gatcagtggc gaggccggga tcggcaagac gacgctggtc gccaacctgg ccgatgtcgc
     3121 ggttgcgcac ggttgtctcg tcctctgggg ccatgcctac gatctgtcgg tgacaccgcc
     3181 atatggcccg tggctggagc tgctgcggcg ctatcctgcc gccaccaacg ggttgccact
     3241 cctcccggcc gtcgtcgagg acggccgctc gctggcgacc gtcggcagcc aggaatccct
     3301 cttcgcgacc gtcgccacct tctttcgcgc tgtcgccaac cagacgccgc tcgttcttgt
     3361 cctcgatgat ctgcactggg ccgatcaggc gtcgctcgac ttcctgcgct acctggcgcg
     3421 gcagatcagc cacgaccgga tcctcctcct cgcaacctat cgctccgacg agatccatcg
     3481 ccaccatccg ctcttcgacc tgctgccact cctcgttcgc gaatccggcg ccgagcggct
     3541 ggaggtccat cggctcgacg ctgcgggaca ccgggcgctg gttgccaacc gctacacgct
     3601 gccgcaggtg gacaccgacc ggctcgtcca gtacctcgag ctgcacgcgg agggcaatcc
     3661 gctgtttgcc ctcgaactgt tgcgcacgct cgaagacgaa ggcaatctcc ggcagacgga
     3721 cgccggctgg tcgcttggcg acgccgcgca ggtccgcgtc ccaccgctgc tgcggcaggt
     3781 gatcgaccgg cggctggccc ggctcggcga caacgcccgt tcgctgctac aggtggcggc
     3841 catcatcggt caggaggtgc cgctcgatct ctggcagcag atcgtcgagg cgagcgatga
     3901 cgcgctgatc gacgcaatcg agcaggggca ggtcaccaac ctgctggacg aggtcgcggg
     3961 cggcgcgagc tatcgattcc agcacgcgtt gctacgggaa gccctctacg aaggcgtcgt
     4021 ctcgctgcgc cggcgcagct ggcaccggaa ggtggcggag gcgctggcaa cacaacccaa
     4081 cccggttccc gacgttgttg cctaccactt ccagcaggcg ggcgatgtcc gcgccgttgc
     4141 ctggttgctg gagtccggac ggcgcgcccg gatggcctac gcaaccgcat cggcaatcga
     4201 ccggttcgag acggcgctgg cactcgacga gcagcacgac ggcgccagcg gcctgcgcgg
     4261 ctggctgttg gcgggactgg caacctgtgg tgcgttgtac tcgaacaccg acgagcgcct
     4321 gcggacactc aacgaggcta tggacttcgc agcccagacg aacgatgacg tcctgatggg
     4381 gttgtgcgag tggtcctcga cgttccttgc aacctccggc ccaggttcag cgctacaact
     4441 ctacaaggca cgagatcgca ttcaggctct gccacctgag gatcggggtc gcctctacgg
     4501 cttcatctac ggatcgaccg gcgtggcact cgatccaagt ggtcctgaca tgacctgtga
     4561 aatcgttgga ctccagggac aatcggggca ggtccgagag acactggcgg ctgtcgagcg
     4621 catccgcgag gagtacccca ggctctcggc gactgccgag ctcggggtcg acaacgcact
     4681 gatgacgagc tttcaggcgc tcggtcgtcc cgacgactcc cttcggcatt acgaacacat
     4741 gcaagcggtg caccgccgta accatgtgac ggactggata gcaatcacaa cctggctcaa
     4801 gctacgcgac ctggtgctgg tctactggcc cgaccggatc gccacgcgcc tggaagtcgc
     4861 aagcgattcc atcgaagcaa tccggctggc caaagctgaa gggacactgg ggccagatgt
     4921 cccggatgaa accggagcca tctggctgta cctggttgat ggccgttggg atgatgcccg
     4981 acgggtcgtt gaagtcgcca cgagcgctgg acaatggatc accttcctga ctgcggcggc
     5041 gtggatgacg ctgacacgac accagggaga tccggaggcg gcattagcgc ggctgccgat
     5101 cacgttcccg gatgggccgg cctcgcagcc gggccggcag accttcgaaa cctcggtgct
     5161 gtgcatgcac gccgctatcg agatcgcgct cgacgccggc gatctggcgt tggcgcgcgc
     5221 ctggctggag tgccacgacc gctggctcga ctggagcggc cacattccct tcaccaccat
     5281 cggtcaccga ctctgggcgc gctatcacca cgtcagcggc gaccgtgccg ccgccatcga
     5341 gcgcgccaac caggcgctgg aactggcatc agacccccgc caaccgttga cgctgctggg
     5401 ggcccaccgt ctgctgggcg aactggagac cgacggcggc aaccacggca atgcccgcca
     5461 gcacctcgcc gaagccctcg cgctggccga cgcctgtcag gcgcccttcg aacgggcgct
     5521 gacgctcgtg gcgctggctg aactcgcgct cgccagcagc gatatcggag gcgcgtcccc
     5581 gcatcttgcg gacgctcgcg ccatctgcga accgctcggc gcccgtccga cgctggagcg
     5641 gatcggcgcg ctggaagcac aactcgccgc tgctcccagg caacgtcatc ccgccggcct
     5701 gagcgcccgc gaggttgagg tcctgcggct ggtggccgag ggactgacgg atgccgaggt
     5761 cgctgagcgg ctcttcctca gccggcggac cgtcagcacc cacctgacct cgatctacac
     5821 caagctcggc gtcaactcgc gaacggccgc cacccgcttc gccgttgagc acgggatcac
     5881 ctgacacggc tgttccggga gggttacgta caactacgta gccggggcca catgcaccca
     5941 tcctgcaggg tcgttcagcg attacgtact ccactggatg tgcgagcgtc gcgttactcc
     6001 tattctcgtt ccatcacaac cgatgacgtg aacgacgccg agaggagccc cgacatgatg
     6061 aacccgaccg atctggatgt ccgctttctg aaccacaccg ccaacgtcag ctacgccgac
     6121 cggttcggca gcctctaccc ggcgacacag ccgcagcagc acagcccccg ccgctacgtc
     6181 gccagcgtgc tgctccgcct gtcggcccgg ctcgctccgc agcaaacaca gcaggccatc
     6241 gcgcgggact ccgccggcgt ctgatcgcgt cattttgtca tcctgaggcg tgagccgaag
     6301 gatctctcct acgttggccg gtcaatcacc cgaggagaga tccttc
//
LOCUS       DASUGJ010000089         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_11793, whole genome shotgun sequence.
ACCESSION   DASUGJ010000089 DASUGJ010000000
VERSION     DASUGJ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11793"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFV93_01995"
     CDS             complement(<1..461)
                     /locus_tag="VFV93_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164141.1"
                     /translation="MTHQHTIVCADGDAGYHALIDDEQRAELARQGIRLVWHNGAPAS
                     DAEWLARIGDAEALLLLWTIPEAVMRALPNLRAIAWVGTGVATFVNIPLANELGIVVS
                     NTPGYGSEAVAEHALGVMLALARNTVALDADVRAGRWPRDDLAGVELAGKVA"
     gene            571..2145
                     /locus_tag="VFV93_02000"
     CDS             571..2145
                     /locus_tag="VFV93_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5164142.1"
                     /translation="MKTYDAVIIGGGHNGLVAACYLAKAGYDVCVLERYHTVGGAAIS
                     EEFEDAPGFIASTGSYVLSLAPQKILNELDVWEHGLELIERNPRFFMPFPDGQYLIYW
                     NEQDRMLEEVRKFSKADADNFAWYDDFVERACQVMDQFILRRPPSFAEFAGAFNQKGD
                     AHVFQKLILGSSADVAEYFFESEQIQAGIAASGLIGTFRGPRDAGTGYVKLYHSMGMA
                     TGSRGRWAYVRGAMGGVTQALASVARSEGVEIRTDAEVEQVIVRDGVATGVALASGEE
                     INGKVVLSNADPKRTYLKLVGPDLLPDQYRRDIEAIQIESPVIKINLAMEELPQFTAL
                     DGDGRQQSWGQTGGLFIAPNIEYMQRAVEDARNGRPSDYPFFNIHSQSAIDPTVAPPG
                     KHTLSIFAQYVPYELAEGSWDERRGEIAQHFMRRFAEYAPNVMDAVISMQVLAPPDIE
                     ARFGLTGGHIFQGELVPEQAFDMRPVPGSSSYEGPIRGLYLCGSGAWPGGCVMGAPGH
                     NAAHEVIDNLRAGRHA"
BASE COUNT          379 a          778 c          719 g          384 t
ORIGIN      
        1 gccaccttcc cggccagttc gacaccagcg aggtcgtcgc gcggccagcg gccggcccgt
       61 acgtcggcgt ccagcgcgac cgtgttgcgc gccagcgcca gcatcacacc cagcgcgtgc
      121 tcggcgaccg cctcgctgcc gtagcccggc gtgttcgaga cgacgatacc aagctcgttg
      181 gccagcggaa tgttcacgaa ggtcgcgaca ccggtgccga cccaggcgat cgccctcagg
      241 ttcggcagtg cccgcatgac cgcttcaggg atcgtccaga gcagcagcaa cgcttcggcg
      301 tcgccgattc gcgcgagcca ctccgcgtcg ctggccggag ccccgttgtg ccagaccagc
      361 cggatgccct gccgcgccag ttccgcccgc tgctcgtcgt cgatcagggc atgatagccg
      421 gcatcgccgt ccgcgcagac gatcgtgtgc tgatgcgtca tccgccacct gccaaccgtt
      481 gaccgcgcca tgcttcgatt gcgatagtat gccagcacat gcgaagttga aatccggtaa
      541 ctgtgcgttg acagcaccag ggagcgagcc atgaagacgt acgatgcggt catcatcggt
      601 ggtggacaca acgggctcgt cgcggcctgc tacctggcga aggcgggcta cgacgtctgc
      661 gtcctggagc gctaccacac ggtcggcggc gcggccatct ccgaggagtt cgaggacgcg
      721 cccggcttca tcgcctcgac cggatcgtac gtcctctcgc tggcgccaca gaagatcctc
      781 aacgaactgg acgtctggga gcacggcctg gagctgatcg agcgcaatcc gcgcttcttc
      841 atgcccttcc ccgacggaca gtatctgatc tactggaacg agcaggaccg gatgctggag
      901 gaggtccgca agttctcgaa ggcggatgcc gacaacttcg cctggtacga cgacttcgtc
      961 gagcgtgcct gtcaggtgat ggaccagttc atcctacgtc gcccgcccag cttcgccgag
     1021 ttcgccggcg cgtttaatca gaagggcgac gcacatgtct tccagaagct gatcctcggc
     1081 tcgtcggccg atgtcgccga atacttcttc gagagcgagc agattcaggc cgggatcgcc
     1141 gcctcgggcc tgatcggcac cttccgtggc ccacgcgacg ccggtacggg ttacgtcaag
     1201 ctctatcact caatggggat ggcgaccggc agccggggcc gctgggccta cgtgcgcggc
     1261 gcgatgggcg gtgtcacgca ggcgctggcc agcgtcgccc gctcggaggg cgtcgagatc
     1321 cggaccgacg ccgaggtcga gcaggtgatc gttcgcgacg gtgtcgcaac cggtgtcgcg
     1381 ctggcgagtg gcgaagagat caacggcaag gtcgtgctct cgaacgccga ccccaagcga
     1441 acctacctaa agctggttgg ccccgatctg cttcccgacc agtatcgccg cgacatcgag
     1501 gcgatccaga tcgagtcgcc ggtcatcaag atcaacctgg cgatggagga gctgccgcag
     1561 ttcacggcgc tcgacggcga tggtcgccag cagtcgtggg ggcagaccgg cggcctcttc
     1621 atcgcgccga acatcgagta catgcagcgc gccgtcgagg atgcccgcaa cggccgcccc
     1681 agcgactatc cgttcttcaa cattcactcg cagtcagcga tcgacccgac cgtcgcccca
     1741 ccgggcaagc acacactctc gatcttcgcc cagtatgtgc cctacgaact ggccgagggc
     1801 agctgggacg agcgccgtgg cgagatcgcc cagcatttca tgcgccgctt cgccgagtac
     1861 gccccgaacg tgatggacgc cgtcatcagc atgcaggtgc tggccccgcc cgacatcgaa
     1921 gcccgcttcg gactgaccgg cggccacatc ttccagggcg agctggtccc ggagcaggcg
     1981 ttcgacatgc gccccgtccc cggctcatcc agctacgagg gcccgatccg cgggctctat
     2041 ctctgcggct ccggcgcctg gcccggcggc tgcgtgatgg gcgcccccgg ccacaacgcc
     2101 gcccacgagg tgatcgacaa tctgcgcgcc ggccgacacg cgtagggtgc tgacggcgcg
     2161 ccaatcgata ttggaggttc gttgtgtttg cagtcgtgcg cgttcttggc ccacacctct
     2221 tcagcgtggt cggacgcttc ggcttcttta tcgccgctat
//
LOCUS       DASUGJ010000090         7965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1122817, whole genome shotgun sequence.
ACCESSION   DASUGJ010000090 DASUGJ010000000
VERSION     DASUGJ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122817"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..534)
                     /locus_tag="VFV93_02005"
     CDS             complement(238..534)
                     /locus_tag="VFV93_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX5164143.1"
                     /translation="MTDTTISASEARRAFGKVLDRVSAGGESIVIERRGEPVAAVASI
                     GDYRQWQSQREAARARLGAFFETVSERADIPPHEADAFSLEVGSHIRATRKSHQ"
     gene            complement(863..2668)
                     /locus_tag="VFV93_02010"
     CDS             complement(863..2668)
                     /locus_tag="VFV93_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164144.1"
                     /translation="MIVRAVMLPVVFWVVVLTFVRLAIVPPEACVSATPAEMRAAAVE
                     AAHWIVRNQLADGRYLYEYNRATDTRSADYNIVRHAGVTASLYQAAGQAEAPDLLPAA
                     DSGTLWMIDHLIHHDDWSALADGQSVPLGASALMLVSLAERRLLTGDEQYDDVMRQLG
                     RFLVTMQRPDGNFSAYWDVTNQQPDAESESIYFPGEANWALALVHEALPGEGFDTAAW
                     AALDYIAMHRDQDAGVRFPPQRDHWSAYALGEMGEWGLTDRQIDYAHGNASRLGLAIR
                     WMSQTESRIGQWFVDWRGGEARGAAFGTWVEGSAALWRLAAIDPRMADLRGDIRERAA
                     CGAGILVKQQVPSDADPIEAGAWFTDDVTRMDDQQHVISGLLYTANAIEGDARREPAP
                     LAGDSRPAPPALVEPEPDRRIADRLMAPLYLILIINPLGAAMVATGFGGRTASLRLGR
                     VWWIGATALVLVAAALAGPFTRWLDISLPSWQLAAGVLVIVAVLRLFVRADPFAPLVD
                     PDIPDWLAIGWGIGWLASPATLAVVMAYSLDLGPFIAMVAAALAVTFASIGALLGPAL
                     LGWLGYRRLRECARWSSLFGLITAATLILAGLNGV"
     gene            2822..3832
                     /locus_tag="VFV93_02015"
     CDS             2822..3832
                     /locus_tag="VFV93_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5164145.1"
                     /translation="MGRDNLRALHAIVMVLLVLSGLLVGVRQATTVAAEQPAYSPRDT
                     LSWPGSPDPRDAAMLPLSAFDGRLAAHAYLVREITSIAIYDPASNRVYRGGDAGPIAG
                     ASLSKVLMAVIALRQFEQRGAGEDEIASVESLIYPTIAYSDNDYANEVWALIGRQPAV
                     AEFVAQTGLTGFSAPDPWDWGQISATAPDWAILFAMLGSGELLNQRNTAAILSMMDDV
                     IEEHRWGVLAEREDSVSYGKNGWYMDEDNAWDWRVNSAGFVSVAEPDHAVTPRVVVVM
                     TRYPADEGMSYGVELATRVTDRVVTCTHTQHARTTLNQRSASCTSNRETSMLMDRVHR
                     IW"
     gene            3928..5268
                     /locus_tag="VFV93_02020"
     CDS             3928..5268
                     /locus_tag="VFV93_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5164146.1"
                     /translation="MSAERNPNILIMTCHDMGRYLGCYGIPTVRTPNLDRLAADGVRF
                     EQAYCTAPQCSCSRAAMYTGRYPHSTGVLGLTHGDFAWDMDADERLIGQALQSAGYTT
                     MLLGIHHETQQGPPDKVAARCGMDEVLPPAPAAQLVDWAIERIQAVQQPFYLQIGFHE
                     PHRIKGSNEPETVMGFTGDEIEPDDELGIHIPPYLADDEGTRTELAELQGALHSLDRE
                     IGRLLEHLDQLGLAGNTLVIATTDHGIAMPRAKCTLYDPGIESALIMRLPARGWTGGR
                     TVSAMTSNVDVFPTLIELAGLPPDERVQGRSLRHLLDGEATTFREHVFSELTYHDYYD
                     PRRAVRSTTHKLIVNFSAAPSFMDPTQSWQPRSRPVVPANPAEAYHPPVELYDLRTDP
                     NEWINLADDPAYATTRAELLVQLLTWMRDTSDPLLDGPVLSPSHERAIRALGGT"
     gene            5406..5633
                     /locus_tag="VFV93_02025"
     CDS             5406..5633
                     /locus_tag="VFV93_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164147.1"
                     /translation="METIDQLLEVKTRLTTIKGYAQLLEREVDRTQPHSAKLDAQVEE
                     LNREIARLVNLVGTIEASMTAGAGSPPAADL"
     gene            complement(5646..5963)
                     /locus_tag="VFV93_02030"
     CDS             complement(5646..5963)
                     /locus_tag="VFV93_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164148.1"
                     /translation="MYARSITFRAQPGVTTRQASEIYEELTRLLADAEGFLGSTFMMN
                     PETNHAISLTFWRDRESGAAAGPKVLPLLLVKVHPLVLSPPEISGYDVIGQTFELNDA
                     TGA"
     gene            6071..6874
                     /locus_tag="VFV93_02035"
     CDS             6071..6874
                     /locus_tag="VFV93_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5164149.1"
                     /translation="MATRETVTINGIDISSLRTGDGPPLVMLHGIGGNASQFQHQIDD
                     LSDAWTVASWDAPGYGSSGDPGDDWLMNDYADALAGWLDALGFDSVSLLGQSWGGVLA
                     QTFVGRYPARVRALVLSDTSMGGRAQPDAERLAGLNARLSALETMTPAQMAAARTPAV
                     LGPNPTTAQLREAETMMAQIRPTGYRRAAIALAEADTRPIHAGIAVPTLILAGEFDKI
                     VRSSTAAELQAAIPGARLVTIAGVGHLSGQEDPKTYNAALRSFLADRDS"
     gene            7028..>7965
                     /locus_tag="VFV93_02040"
     CDS             7028..>7965
                     /locus_tag="VFV93_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5164150.1"
                     /translation="MSASAPTTDSHMYQTMHSQPADVQRLLDEGWKPAEEAAGLIANA
                     ERVFLTGIGTSYHAALVGSWLLRAAGCDARAVNSFDMAHYRESMGVRPSDAVILMAHT
                     GVKQISSDALKAAVNADATVISVGNTTAEHPGSQLILRSVRPEQSAAYTSSHVCAMTV
                     LAQIATVLGEQRGATATAGFREALTALPGQIEGVLGRQDEILPISSAATTARVYAAGA
                     GPNEATALESVIKVREAAYGWIDALALEQFMHGPIVAANAGDMAALVNVSGPAEERTG
                     QIAAMLSAVGVKVWLIGKPAPAAPDATVFSLPETDEL"
BASE COUNT         1412 a         2826 c         2507 g         1220 t
ORIGIN      
        1 tcctgatcgg aaacccgctt gtcaatcgcg ctcatcctgc gcctcccgcg cctgttgagg
       61 atgccgcagt cacgttccac ggcacggtcg ctctcggcgg ccactaccgc cgcctcccag
      121 tatcaggatc acacacaacc acctctgtac actactgtac cagccacctg tacagcgcga
      181 tacagacaag ttcgaaaacg ccttccccgc caaggatttc gtgttgagtt cgtcctctca
      241 ttgatgtgat ttccgagtcg ctctaatgtg ggagccgact tcgagtgaaa atgcatcagc
      301 ttcgtgtggc ggtatgtccg cacgctcgga aactgtctcg aaaaatgccc ccagccgagc
      361 acgagccgct tcacgttgcg actgccactg tcgatagtcg ccgatcgaag cgactgcagc
      421 gaccggctcg ccccgccgct cgatgacgat actctctcca ccagccgaca cgcggtcgag
      481 caccttgcca aacgcccgcc gagcttcgga cgcgctgatc gttgtgtcgg tcatgatagc
      541 ttcacccttc tgcggtgcag aaagcgctcc aagtatcatg cactgtcgac acctgtcacc
      601 ggaatcggaa cggtaaaaaa aagccgggac gaccggcaag gacggtcgcc ccggcgagga
      661 ggagaggagg agagagctcc taccctatgt aacgcatgaa catcgccaca gttgcgctgg
      721 gcgggcttcg gtgtaggatg atgaatgaaa gcgctggcag acgtggaatc tctcctcctc
      781 acacgtccgg cagcgctttc cttctcggtg tacgcccgca gccacgcgag tggatgcacc
      841 ggcgacagga ctgtcgcccg tctcagacgc cattcaggcc ggcgaggatc agcgtcgcgg
      901 ccgtgatcag gccgaacagg ctcgaccagc gggcgcattc gcgcaggcgg cggtagccca
      961 gccagccgag cagggccggg ccgagcaagg cgccgatcga ggcgaacgtc accgccagcg
     1021 cggccgcgac catcgcgata aacggcccca gatcgagcga ataggccatc acaaccgcca
     1081 gcgtcgccgg tgacgccagc cagccgatgc cccaaccgat cgccagccag tcggggatgt
     1141 ccggatcgac gagcggcgcg aacggatcgg cccggacgaa caggcgcagg acggcaacga
     1201 tgaccaacac accggccgcc agttgccagc tcggcaggct gatatccagc cagcgcgtga
     1261 acggcccggc cagcgccgcc gcgacgagca cgagcgccgt cgcgccgatc caccagacgc
     1321 gacccagccg caggctggcg gtccgcccgc cgaacccagt tgcgaccatc gccgcgccga
     1381 gcgggttgat gatcaggatc aagtacagcg gcgccatcag ccggtcggca atccgccggt
     1441 caggctccgg ttcgaccagc gccggtggcg ccgggcggct atcgccggcc agcggcgccg
     1501 gctcgcggcg ggcgtcgccc tcgatcgcgt tggccgtata gagcaggccg gagatgacat
     1561 gctgctggtc gtccatcctg gtcacgtcgt cggtgaacca ggcgccggcc tcaatcgggt
     1621 cggcgtcaga cgggacctgc tgcttgacga ggatgccggc gccacaggcg gcccgctcgc
     1681 ggatatcgcc gcgcagatcg gccattcggg gatcaatcgc cgccagccgc cagagagcgg
     1741 cgctgccctc gacccaggtc ccgaacgccg ccccgcgcgc ctcgccgccg cgccagtcga
     1801 cgaaccactg gccgatccgt gattcggtct gcgacatcca gcggatggcc aaccccaacc
     1861 gggacgcgtt gccatgcgcg tagtcgatct gccggtcggt cagcccccac tcacccatct
     1921 cgccaagcgc gtaggccgac cagtggtcgc gctgcggcgg aaaccggacg ccggcatcct
     1981 ggtcgcggtg catggcgatg tagtccaacg ccgcccatgc tgccgtatcg aagccttcgc
     2041 cgggcagcgc ctcgtgcacc agcgccagcg cccagttcgc ctcgccgggg aagtagatcg
     2101 actcggactc ggcatccggc tgctggttcg tgacgtccca gtaggcgctg aagttgccat
     2161 ccggccgctg catcgtgacc aggaagcggc cgagctggcg catgacatcg tcgtattgtt
     2221 cgtctccggt cagcagccgc cgttccgcca gcgagacgag catcagtgct gatgcgccga
     2281 gcggaaccga ctgtccgtct gccagcgccg accagtcgtc gtggtggatg agatggtcga
     2341 tcatccagag tgtcccgctg tcggctgccg gcaacagatc gggcgcctcg gcctgaccgg
     2401 cagcctgata gagcgaggcc gtcacgccgg catggcggac gatgttgtag tcggccgagc
     2461 gggtatctgt cgcgcggttg tactcgtaca gatagcgacc gtccgccagc tgattgcgga
     2521 cgatccagtg ggcagcctcg acagccgctg cccgcatctc ggccggtgtc gcgctcacgc
     2581 aggcttccgg cggcacaatc gccagccgga cgaacgtcag gacaaccacc cagaacacaa
     2641 ccggcagcat caccgctcgt acgatcatcg ccctctgctc tcctgcatcg cgtgaacgga
     2701 tcgtcgcacg gagcggccgt tggcgcaatc cggcacccta atcgtaccga ttggccgtac
     2761 gtacagcttg cgctgcgcgg tcccgcgact aggataggag tctggggaca cctgtggggt
     2821 cttggggagg gacaatctgc gcgcacttca tgccatcgtg atggttctgc tggtgctgtc
     2881 cggcctgctg gtcggcgtca ggcaggcaac caccgtcgcc gccgagcaac cagcctattc
     2941 gccgcgcgac acgctctcct ggccgggatc gcccgatcca cgcgacgccg cgatgctccc
     3001 gctgtcggcc ttcgatggtc gactggccgc ccacgcctac ctcgtccgcg agatcaccag
     3061 catcgccatc tacgacccag cctccaaccg cgtctaccgt ggcggcgacg cgggaccaat
     3121 cgccggtgcg agcctctcga aggtcctgat ggccgtcatc gccctccggc agttcgaaca
     3181 gcgtggagcc ggcgaggacg agatcgcctc ggtcgagtcg ctgatctacc caacgatcgc
     3241 ttactcggac aacgactacg ccaacgaggt ctgggcgctg atcgggcggc aaccggccgt
     3301 cgccgagttc gtcgctcaga ccgggctgac cggcttcagc gcgcccgacc cgtgggactg
     3361 gggccagatc tcggcgaccg cgcccgactg ggccatcctc ttcgcgatgc tcggcagcgg
     3421 cgagctgctc aaccagagaa atacggccgc gatcctctcg atgatggacg acgtcatcga
     3481 agagcaccgc tggggcgtgc tggccgagcg cgaggacagc gtcagctacg gcaagaacgg
     3541 ctggtacatg gacgaggaca acgcttggga ctggcgtgtc aacagcgccg gcttcgtctc
     3601 agtcgccgag ccggaccatg ctgtcacacc ccgcgtcgtc gtcgtcatga cccgctaccc
     3661 tgccgacgag ggcatgtcct acggcgtcga gctggctact cgcgtcaccg accgcgtcgt
     3721 cacctgcacc cacacccagc acgcccgcac aacactcaac cagcgctcgg catcctgcac
     3781 gtccaaccgc gagacctcga tgctgatgga ccgcgttcat cggatctggt aacgcggggg
     3841 gaggggtgcg ggacgcgttc cgcgttccac atcccgcgct agcatgcatc ctgcgtcgag
     3901 ccgatcggag acgagaggat gtcgctgatg agcgcggagc ggaatcccaa tatcctgatc
     3961 atgacctgtc acgacatggg tcgctacctc ggttgctacg gcatcccgac cgtcaggacg
     4021 ccgaacctgg accgattggc cgccgatggc gtccgcttcg agcaggcgta ttgcacggcc
     4081 ccgcagtgca gttgctcgcg ggccgcgatg tacaccggcc gctacccgca ctcgaccggc
     4141 gtcctcggct tgacgcacgg cgacttcgcc tgggacatgg acgccgacga gcgactgatc
     4201 ggccaggcgc tgcaatcggc tggctacacg acgatgcttc tcggtatcca ccatgagacg
     4261 caacaggggc cgcccgacaa agtcgcggcc cgctgcggga tggacgaggt gctaccgccc
     4321 gcgccggcgg cccaactggt cgactgggcg atcgagcgaa tccaggccgt ccagcagccg
     4381 ttctacctcc agatcggctt ccacgagcca caccgcatca agggcagcaa cgagccggaa
     4441 acggtgatgg gcttcaccgg cgatgagatc gagccggacg acgaactggg catccacatc
     4501 ccaccctacc tcgccgatga cgaggggacc cggacggaac tggccgagct acagggcgcc
     4561 ctccatagcc tcgaccggga gatcggccgg ttgctggagc atctcgacca gcttgggcta
     4621 gccggcaaca cgctcgtcat cgcgacgacc gaccacggca tcgcgatgcc gcgcgccaag
     4681 tgcacgctct acgatcctgg catcgagtcg gcgctgatca tgcgcctgcc ggccagaggc
     4741 tggaccggcg gccgaaccgt ctccgcgatg acctccaacg tcgatgtctt cccgacgctg
     4801 atcgaattgg ccggcctgcc gcccgacgag cgtgttcagg ggcgcagcct gcgacacctg
     4861 ctcgatggcg aggccacgac attccgtgag catgtcttca gtgaactgac ctaccacgac
     4921 tactacgatc cgcgccgcgc ggtccgcagc acaacccaca agctgatcgt caacttcagc
     4981 gccgcgccat ccttcatgga cccgacccag tcgtggcagc cacgctcgcg gccggtcgtt
     5041 cctgccaacc ctgccgaggc ctaccacccg ccggtcgagc tctacgactt gcgaaccgac
     5101 cccaacgagt ggatcaacct ggcagacgat cccgcctatg caaccacccg cgccgaactg
     5161 ctcgtccagc tcctgacctg gatgcgcgac accagcgatc cgctgctgga cgggccggtt
     5221 ctctcgccgt cccacgagcg cgcgatccgg gcgctgggcg ggacgtgagg cgctccgtat
     5281 cacccgtcct gctcgacttg gcacagcccc tgcatagtca ccactgtccg gcgacccggc
     5341 cggaccctgt gagcgcccag atcagcgtcc ttgccggcag gaatgagaga tgtcaggggc
     5401 caccagtgga gacaatcgac cagcttctcg aagtcaagac ccggctgacg acgatcaagg
     5461 gctatgccca actcctggag cgtgaggtcg atcggacgca accgcactcc gccaagctcg
     5521 acgcacaggt tgaggagctg aaccgcgaga tcgcgcggct ggtcaatctc gttggaacga
     5581 tcgaagcgtc gatgacagca ggagccgggt cgccgccagc agccgacctc tgagccggta
     5641 gtcgctcagg cgccggtggc gtcgttcaac tcaaacgtct ggccgatcac gtcgtaaccg
     5701 ctgatctccg gcggggacag caccagcggg tggaccttca ccagcagcag cggcagcacc
     5761 ttcgggccgg ctgccgcgcc cgactcccgg tcgcgccaga acgtgagcga gatggcgtgg
     5821 ttcgtctcgg gattcatcat gaaggtcgaa ccaaggaacc cctccgcatc ggcaagtagc
     5881 cgcgtcagct cctcgtagat ctccgacgcc tgccgggtcg tcacgccggg ctgcgcgcgg
     5941 aaggtgatcg atcgtgcgta catgcctgtc ctccctggtg aggcggcgtt cgtgctgcat
     6001 gtctccatct tcgcatactc gtgctaccct gcgccgcaga ggcagccgcc gcaggacagg
     6061 agcaacagcg atggccacgc gcgagacggt caccatcaac ggaatcgaca tcagctccct
     6121 gcggaccggc gatggtccgc cgctcgtcat gctgcacggc atcggcggca acgcgagcca
     6181 gttccagcac cagatcgacg acctctccga cgcctggacc gtcgccagtt gggacgcccc
     6241 cggctacggc agctccggcg atccaggcga cgactggctg atgaacgact acgcggacgc
     6301 gctggccggc tggctcgacg cgctcggctt tgacagcgtc agcctgctcg ggcagtcgtg
     6361 gggcggcgtt ctggcccaga cgttcgttgg ccggtacccg gcgcgggtcc gggcgctggt
     6421 tctctccgac accagcatgg gtggtcgcgc gcagcccgac gccgaacggc tcgccggcct
     6481 caacgcccgc ctcagcgcgc tcgagacgat gaccccggcc cagatggcgg ccgcccgcac
     6541 accggccgtc ctcggtccaa acccaacaac cgcgcaactc cgcgaggcgg agacgatgat
     6601 ggcccagatt cgtcccacgg gctaccgtcg ggccgcgatc gcactcgccg aggccgacac
     6661 gcgaccgatc catgcgggca tcgccgttcc gacgctgatc ctcgccggtg agtttgacaa
     6721 gatcgtccgg tccagcacgg ccgctgagct gcaagccgcc atccccggcg ccaggctcgt
     6781 cacgatcgcg ggcgtcggcc acctcagcgg ccaggaagac ccgaagacct acaacgccgc
     6841 gctacgatcg ttcctggcag atcgtgacag ttgaacacac aggtgatgac gtgttaccgg
     6901 atggttccgc caactcgctg aaagtggccc tccacgtcag agccacattg cgctaccctg
     6961 cccggcagcg tcgcggtacg atcgcgcgca tctgattctg cgaattcacc ggggatgggg
     7021 gctgagcatg agcgcgagcg cgccgacgac cgattctcac atgtatcaga cgatgcactc
     7081 gcagccggct gacgtgcagc ggctgctcga cgagggctgg aaaccggctg aggaagccgc
     7141 cgggctgatc gccaacgccg aacgtgtctt cctgaccggc atcggcacca gctaccacgc
     7201 ggcgctggtc ggctcctggc tgctgcgggc agccggctgc gacgcgcggg ccgtcaactc
     7261 cttcgacatg gcccactatc gcgagtcgat gggcgtccgc ccgagcgatg ccgtcatcct
     7321 gatggcacac accggcgtca agcagatctc gtccgacgcg ctcaaagccg ccgtcaacgc
     7381 agacgcgacc gtcatctcgg tcggcaacac gacggccgag caccccggct cgcaactgat
     7441 cctgcgctcg gtccggccag agcaatcggc cgcctacacc tcttcgcacg tctgcgcgat
     7501 gacggttctc gcccagatcg cgaccgtcct cggagagcag cgcggtgcga cggcgaccgc
     7561 cggattccgt gaggcgttga ccgccctgcc tggccagatc gaaggagttc tcgggcgtca
     7621 ggacgagatc ctgccgatca gcagcgccgc gacaacggcc cgcgtctacg cggccggggc
     7681 cggcccgaac gaggcgacgg cgctcgaatc ggtcatcaag gtccgcgagg ccgcctacgg
     7741 ctggatcgac gcgctggcgc tggagcagtt catgcacggc ccgatcgtgg ccgccaacgc
     7801 cggtgacatg gccgccctcg tcaacgtcag cggccctgcc gaggagcgca ccggccagat
     7861 cgccgcgatg ctctcggctg tcggcgtcaa ggtctggctg atcggcaagc ccgctccggc
     7921 cgcgccggac gcgaccgtct tcagcctgcc tgagaccgac gaact
//
LOCUS       DASUGJ010000091         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1678936, whole genome shotgun sequence.
ACCESSION   DASUGJ010000091 DASUGJ010000000
VERSION     DASUGJ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678936"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="VFV93_02045"
     CDS             complement(<1..1301)
                     /locus_tag="VFV93_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164151.1"
                     /translation="MSQHSTPTRTGTVAPTATTSPPALLPILLGLLERSRRAVGQERV
                     FARLVVLTLGMISALGRHTITQLLVAVGQGEADWSGFYRLFSQGRFQPDAAGVALVGE
                     TLADVPATAPYLVVVDGVQLPRQSQRLPGTSWLKCPRTPPWKPGIHRAQRYSHLAWLT
                     PSSADGDRRAVPLRVIPAPPPKAIRPSDAAQADACGLTAMREWEAGLASLNWLRQTLD
                     AAGRAAQQILAVADGSYSGARMWMGLPARTTLLARCAKNRALYALPTAHPGRGRPRKY
                     GERAPSPADWLSERCGWSRTTVRVRGRSIPVTYRVAGPYLVKPAATQPLYLIVVRGID
                     RRPGHKHRPPAFWLVNAVPTHTDPAGVATEDWQLPYPVTELLAVAWQRWEIEVCHRQA
                     KTSLGVGDAQCWTALGTVRAAQFQWWVYALLVLTAYRAWGHG"
     gene            complement(1453..>2326)
                     /locus_tag="VFV93_02050"
     CDS             complement(1453..>2326)
                     /locus_tag="VFV93_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164152.1"
                     /translation="DDGGSDVPVIGPSFTEDPDWNGPVKVIDTNVADALVCVYAAGDV
                     GQGGNPDLADITEEDCELLLGTGKGGGNAGQGEPDTYFANLGENRTAAIAFDAESQEL
                     DVTIQVDDLLAGSDYSAAIAGFGCDLNFDGDCADDGEDGGTNAVCSPDAGATLPNPYS
                     GDVLVDLGIATADGDGDLALSFTGVLDAFETQQLGDFEDNVVVVMDDTGAIVACGIIE
                     SGSVGLETPEEAAIRLGAVVVGVADGSGDIAFGLPAGDYYAVINADGYQLAVEEFTLD
                     ADETELNEVNLISD"
BASE COUNT          373 a          805 c          776 g          372 t
ORIGIN      
        1 ccatgccccc aggcccgata ggccgtgagc acgagcaacg catagaccca ccactggaac
       61 tgcgccgccc gcaccgtccc cagcgccgtc cagcactgcg cgtcgccgac cccgagactg
      121 gttttcgcct gccggtgaca gacctcgatc tcccagcgct gccaggcgac cgccagcaac
      181 tcggtcaccg gatacggcag ctgccagtct tcagtggcca ctccggcggg atcagtgtgc
      241 gtgggaacgg cgttcaccaa ccagaaggcg ggtgggcggt gcttgtgacc aggccgtcgg
      301 tcgatgccac gcacgacgat gagatacagt ggctgcgtgg cggccggctt gaccagatac
      361 ggccctgcca cgcgataggt gacgggaatc gagcgaccgc gcacgcgcac cgtcgtccgc
      421 gaccagccac agcgctcgct gagccagtcc gctgggctgg gcgcacgctc gccatatttg
      481 cgcggccggc cccgtcctgg gtgcgcggtt gggagcgcgt acagcgcccg attcttcgcg
      541 cagcgcgcca gcagggtcgt gcgcgccggc aaccccatcc acatccgcgc accactgtag
      601 ctgccatcgg cgacggccag aatctgctgc gcggcacgac cagccgcgtc cagcgtctgg
      661 cgcaaccagt tcaggctggc cagcccagcc tcccactcgc gcatggccgt caagccacac
      721 gcgtcagcct gggcggcgtc gctgggacgg atcgccttgg gtggtggtgc cgggatgacc
      781 cgcaacggca ccgcgcgccg gtccccatcc gcggaactgg gcgtcagcca ggccagatga
      841 ctgtaccgct gtgcccggtg gatgcctggt ttccagggtg gggtccgtgg acatttgagc
      901 caactggtcc ccggcaggcg ctggctctgc cgggggagct gcacgccatc gacgacgacg
      961 agatacgggg cagtggcggg cacgtcggcc aaggtctcgc caaccagcgc caccccggcg
     1021 gcgtcgggct ggaagcggcc ctggctgaac agccggtaga agccactcca gtcggcctca
     1081 ccctgcccaa ccgcgaccag cagctgcgtg atggtatgcc gaccgagcgc actgatcatg
     1141 ccgagcgtga gcacgacgag ccgggcaaag acccgctcct ggcccaccgc ccgacggctc
     1201 cgctcgagca gccccaggag gatcggcagc aacgctggcg ggctggtggt cgccgtcggc
     1261 gcgaccgtgc cggtgcgggt gggcgtgcta tgctgggaca tcgggcatcc tttccatgat
     1321 tgccggcgtg tcacaacccg caatctacga ctggatgccc gtgctgccta gtccccctct
     1381 gcaaagcgcc aaagtccagg ggcgatattg cgatatcgcc cgtaccggat tgatgattgg
     1441 gattgtgtcg cgtcagtcgc tgatcaggtt gacctcgttc aactcggttt cgtcggcatc
     1501 cagcgtgaac tcttcgacgg cgagctggta gccgtcggcg ttgatgacgg cgtagtagtc
     1561 gcctgccggc aggccaaagg cgatgtcacc gctgccatct gctacgccaa cgacgacggc
     1621 gccgagccgg atggcggcct cctccggcgt ctcaaggccg accgagccgg actcgatgat
     1681 gccgcaggcg acgatcgcgc cggtgtcgtc catcacaacc acgacgttgt cctcgaaatc
     1741 gcccaactgc tgggtctcga aggcgtccag gacgcctgtg aacgagagcg ccagatcgcc
     1801 gtcgccgtcg gctgtcgcga ttccgagatc gaccagcacg tcgccggagt agggattcgg
     1861 cagcgtcgcg ccggcgtcgg gcgagcagac ggcgttggta ccgccatcct cgccgtcgtc
     1921 ggcgcaatcg ccgtcgaagt tgagatcgca gccgaagccg gcaatggcgg cactgtagtc
     1981 gctaccggcc agcaggtcgt cgacctggat cgtgacatcc agctcctggc tctcagcgtc
     2041 gaaggcgatg gcggccgtcc ggttttcgcc gaggttggcg aagtaggtgt ccggctcgcc
     2101 ctggccggcg ttgccgccgc ccttgccggt gccgagcagc agctcgcagt cctcttcggt
     2161 gatgtctgcc agatccggat tgccgccctg cccgacatcg ccggcggcgt agacgcagac
     2221 cagtgcatcg gcgacgttgg tgtcgatcac tttgaccggg ccgttccagt cgggatcttc
     2281 ggtgaaactg ggaccgatga ccggcacatc gctgccgccg tcatcg
//
LOCUS       DASUGJ010000092         3675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_984977, whole genome shotgun sequence.
ACCESSION   DASUGJ010000092 DASUGJ010000000
VERSION     DASUGJ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984977"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1332
                     /locus_tag="VFV93_02055"
     CDS             274..1332
                     /locus_tag="VFV93_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5164153.1"
                     /translation="MIRPISAIFGLFSRDLGIDLGTANTLVYVRGKGIVISEPSVVAV
                     DTKSKRALAVGVEAKAMVGKTPETIVAVRPLKDGVIADFDTVELMLHHFIRKVHVQRI
                     MAPHPRVVVGIPSGVTEVEKRAAKDAALSAGAREAYTIEEPMAAAIGAGLPINEPVGS
                     MIVDIGGGTTEVAVISLGGIVVNHSIRVAGDEIDESIIHYARRDHNLVIGERSAENAK
                     ISAGSAYPLEEEKRVTLRGRDLLTGLPKSVEVSSVEIRDAISGPVNTIVEIVKTCVEE
                     TPPELVADIMEQGIILAGGGALLLGLDRRLQAELRMPVRRAEDPLTCVARGTGRVAEA
                     LHLYSRALAGGQELRRGM"
     gene            1347..2183
                     /gene="mreC"
                     /locus_tag="VFV93_02060"
     CDS             1347..2183
                     /gene="mreC"
                     /locus_tag="VFV93_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX5164154.1"
                     /translation="MLRSLPFRRSTLVLLAGLVLLALFLMLLDSRNQLDGPKSLVVTI
                     VSPIGETLTDLGDQVSNIGEGGNENLRQQLADVTEERDQLLHENAQLREQGREVEQLR
                     DLLEFRQANPQLALVTAEVVSRDPGSREQYVIINQGSNAGIEVGMPVLSPHFLVGQVT
                     EVEPDRARVLLIIDSSFQTGARIQGLDAEGIVYGRWQFGERVVMRHLPVDVQVSTDAL
                     VVTSGKTDRVPPGLVIGKVMKVDRDEVENETTLDILPLVDFAELSSVTVITGESGETG
                     TP"
     gene            2180..3604
                     /locus_tag="VFV93_02065"
     CDS             2180..3604
                     /locus_tag="VFV93_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEX5164155.1"
                     /translation="MTRDARLLAAMAASKALTTGLRVSRRGGGTALPGLVANRIDPRL
                     LDKLAGRLPSGAIVVAGTNGKTTTTRMLAGMLESAGRSVVHNRSGSNLVRGISSAFAE
                     QMTLFGTGGAEIGVIETDENAFPDVVRRVQPRVILLLNLFRDQLDRYGELETIANHWR
                     GVIRDLTNDVTLVVNADDPNLAALAAESGARVVTFGLDETGYELPELPHASDAAVCRV
                     CESPLVYDALFVSHLGDYHCPNCGNRRPPLDIVAREIAIRGTESLTMVVRDERDGHEA
                     HEVQAEVALPGVYNAYNALAAVAAARVLGVGFRPILETLRTFRAAFGRLERVEYAGRS
                     LLLALAKNPVGFNEVLRMMTASPLTGPLVIGINDLDADGRDVSWLWDVDFEVLAGEQH
                     SAPIFAVGIRGHDLAVRLKYAGVPTARLDACAAAKPLADALDHVINQTDTGDEIFLLL
                     TYTALLQLRQSLADRGAVAEFWEQ"
BASE COUNT          609 a         1236 c         1212 g          618 t
ORIGIN      
        1 tgccgtcagc atcgccgctc gacccgtcct caggacccca cgctatgcca aacgcaagtg
       61 ccaacgccgt tcatccgatt aagttggaac aggtgggctg cggccatcgc ccctacgcga
      121 gaggaccaac cccgccaccc gtaagtgtta gacttcgtgc gcgccagacc agacgggcgg
      181 tgctccattt tcgcggcctg atcgcggatt cgtcacccct gtgtccggtg gaatgggagc
      241 cgtgcgcttg gcaatattga ggatgagacg cggatgatcc gcccgatcag tgcaatcttt
      301 ggactgttca gccgcgacct cgggatcgac ctcggtacgg ccaacacgct cgtctacgtt
      361 cgtggcaagg gcatcgtcat ctcagaaccg tctgtcgtgg ccgtcgacac caagagcaag
      421 cgcgcgctgg cggtcggcgt cgaggccaag gcgatggttg gcaagacgcc ggagacgatc
      481 gtcgccgtcc ggccgctcaa ggacggtgtc atcgctgact tcgacaccgt cgagctgatg
      541 ctgcatcact tcatccgcaa ggtccacgtc cagcgcatca tggcgccgca cccgcgcgtc
      601 gtcgtcggca ttcccagtgg cgtgaccgag gtcgagaaac gggccgccaa ggacgccgcc
      661 ctctcggccg gtgcccgcga ggcctacacg atcgaggagc cgatggcggc ggcgattggc
      721 gccggactgc cgatcaacga gccggtcggc agcatgatcg tcgacatcgg cggcgggacg
      781 accgaggtgg ccgtcatctc gctcggcggc atcgtcgtca accactcgat tcgcgttgcc
      841 ggcgacgaga tcgacgagtc gatcattcac tacgcccggc gcgaccacaa cctcgtcatc
      901 ggcgagcgct cggctgagaa tgccaagatc tcggccggct cggcctaccc gctcgaagaa
      961 gagaagcgcg tcacactgcg gggccgtgac ctgctgactg gcctgccgaa gtcggtcgaa
     1021 gtctccagcg tcgagattcg tgacgcaatc agcgggccgg tcaacacgat cgtcgagatc
     1081 gtcaagacct gcgtcgagga gacgccgccg gagctggtcg ccgacatcat ggagcagggc
     1141 atcatcctgg ccggtggcgg cgcgctcctg ctcgggctcg accggcgctt gcaggctgag
     1201 ctgcggatgc cggtccgccg ggccgaagac ccattgacat gcgtggcgcg cggaaccggc
     1261 cgcgtcgccg aagcgttgca tctctactcg cgtgcactgg ctggcgggca agagctgcga
     1321 cgtggcatgt agccggttga gttgtcatgc tgcgtagcct gccctttcgc cgctcgacgc
     1381 tggtgctgct ggctggtctg gtcctgcttg cgctcttcct gatgctgctc gacagtcgca
     1441 accagctgga tggcccgaag agcctggtcg tgacgatcgt ctcaccgatc ggcgagacgt
     1501 tgaccgacct cggcgatcag gtctccaaca tcggcgaggg cggcaacgag aatctccgcc
     1561 agcaactggc ggacgtcacc gaagagcgcg accagctgtt gcacgagaac gcgcagttgc
     1621 gcgagcaggg ccgagaggtc gagcagctgc gtgacctgct ggagttccgc caggccaacc
     1681 cgcaactcgc gctcgtcacg gctgaggtcg tcagccgcga tccaggcagc cgcgagcagt
     1741 acgtcatcat caatcagggc tcgaatgccg gaattgaggt cgggatgccg gtcctcagcc
     1801 cgcactttct agtgggccag gtcaccgagg tcgaaccgga ccgcgcccgt gtcctgctga
     1861 tcatcgactc cagcttccag accggtgcgc ggatccaggg actcgacgcc gaagggattg
     1921 tctacgggcg ctggcagttc ggcgagcggg tcgtcatgcg ccacctgccg gtcgatgtcc
     1981 aggtcagcac cgacgcgctg gtcgtcactt ccggcaagac tgatcgcgtc ccgcccggac
     2041 tggtaatcgg caaggtgatg aaggtcgatc gtgatgaggt cgagaacgag acgacgctcg
     2101 acatcctgcc gctggttgac ttcgccgagc tctcatcagt gacggtcatc accggcgaga
     2161 gcggcgagac gggcacgcca tgacgcgcga cgctcgcctg ctggccgcga tggccgccag
     2221 taaggcgctg acgactggcc tgcgcgtctc ccgccggggt ggggggacgg cgctgccggg
     2281 gctggtggcc aaccggatcg acccgcgcct gctcgacaag ctggccggac ggctcccatc
     2341 cggcgcaatc gtcgtggccg gcacgaatgg caaaacgacg acgacgcgga tgctggccgg
     2401 gatgctggaa agcgccggca gaagcgtcgt ccacaaccgc tccggctcga atctcgtccg
     2461 tggcatctcg tcggccttcg ccgagcagat gacgctgttc ggtaccggcg gcgccgagat
     2521 cggcgtcatc gagaccgacg agaacgcgtt tccggacgtc gtccgccggg tccagccgcg
     2581 cgtcatcctg ctgctcaacc tgttccgcga ccaactcgac cgctacggcg aattggagac
     2641 gattgccaac cactggcgcg gcgtcattcg cgatctgacc aatgacgtga cgctggtcgt
     2701 caacgccgac gacccgaacc tggccgcgct ggcggccgag tctggcgcgc gcgtcgtgac
     2761 cttcgggctg gatgagactg gctacgagct gcccgaactg ccgcatgcct ccgacgccgc
     2821 cgtctgtcgc gtctgcgagt cgccgctcgt ctacgacgcg ttgtttgtct cccatctcgg
     2881 cgactaccac tgccccaact gtggcaaccg gcgcccgccg ctggacatcg tcgcccgcga
     2941 gatcgcgatt cgagggacgg agagcctgac gatggtcgtt cgcgacgagc gagacggcca
     3001 cgaggcgcac gaggtccagg ccgaggtcgc gttgccgggg gtctacaacg cctataacgc
     3061 gctggcggcc gtcgcggcgg cgcgcgtcct cggcgtcggg ttccggccaa ttctggagac
     3121 gctgcgaacc ttccgggccg ccttcggccg gctcgaacgg gtcgagtacg ccggccgttc
     3181 gctgctgctg gcgctggcca agaacccggt cggcttcaac gaggtgctgc ggatgatgac
     3241 ggccagcccg ctgaccggtc cgctcgtgat cggcatcaac gacctcgacg ccgacggccg
     3301 cgatgtctcc tggctctggg atgttgactt cgaggtgctg gccggcgagc agcacagcgc
     3361 gccgatcttc gcagtcggca tccgcgggca tgacctcgcc gtccggctca agtacgctgg
     3421 tgtcccaacc gcgcgacttg atgcctgcgc ggcagccaaa ccgctggccg acgccctcga
     3481 ccacgtcatc aaccagaccg acaccggcga cgagatcttc ctgctgctga cctacacggc
     3541 gctgctccag ctccgccagt cgcttgccga ccgtggcgcg gtcgccgagt tctgggaaca
     3601 gtagtctcag tcgcacgccg ttgacaacga cgcgggcttg cgggttcact ccctgtcagg
     3661 catcgtcgtt tgtct
//
LOCUS       DASUGJ010000093         4212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_846462, whole genome shotgun sequence.
ACCESSION   DASUGJ010000093 DASUGJ010000000
VERSION     DASUGJ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846462"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /locus_tag="VFV93_02070"
     CDS             complement(<1..1853)
                     /locus_tag="VFV93_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX5164156.1"
                     /translation="MSAAHARPKARAKARPAATHAPAPARSSAGHAPRSAALGPHYAA
                     GPVGGLALPAQTKLAVGQPGDAYEQQANAVANHVTSGSQLAPPSISPVTAGALARETA
                     TGPEKKKPEPTPAQKEAKPEEKKPAQTPVQKAGIPEPEKKEPEQKLAQRETAKTSKPE
                     EKKPEAAVQKEETNKPPEKNPDPRAIQRAETTTPEDREAESESVQTAPEKAGTATPTS
                     AAGQTGSTTPAADRAIGQAGSGRPMNDSTRKTLESRMDADLGDVRVHDDAESQEASRE
                     LNARAFTHGRDIWLGSGETDRDLGLMAHEATHVVQQDAGAGVQRQMIQRQPAAQAAVA
                     EPAAATLDATTAVPDTRVQRSVATASTIGDGAAPPPAAGAGSSSPNTPVVQRDGIKPA
                     PGGGTGAPGGPWDFEDKAGKLKVGSKKMVIPLMRVPTFKSKITKAPFVLKASGEERED
                     TQRATWEKEARGGSGIDSKLTKKIKDENAPALSKSGQPIYFLQLKGKPIYIIGNQTTI
                     KNRSLRPYWDKTGRPLSFQVDHKHELQLGGGEGIDNLWLLEADANMSSGRNIRSEKNE
                     RIQKLIDAATKKKVANVPDLETARKSYEVTVESVRGGLPVDGQPDKHWEIK"
     gene            complement(1899..4034)
                     /locus_tag="VFV93_02075"
     CDS             complement(1899..4034)
                     /locus_tag="VFV93_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5164157.1"
                     /translation="MQSAFADMPDTARNHFRLNVYVALYDLVYYLRHLGPTSGDNLRQ
                     MLERYPFLKRYFEAMFAYMPDDLSWDATREWWEVELKEWEAAADAHLPLVALAKHAGL
                     SFESRIAAVMVGLVEEDSRFGTLFSDIQRPLSERRPGLELIGQAVQERTSGSGLHAWS
                     ICRPLVEAGIVEAINRDAPRSEWVLRVPPLIWDAIRGEASATPAPWLRTIDDSDFPRI
                     SELIFPDSFVAQLRQLPALIENGRVTAVVVRGMQGSDRLQVLGAVARAMGHGVAYVDL
                     TAAQPQSAEPHWPYLGPFCAAARLLPVIGYDLGPGETVELPALTGYSGPVGVLMGTEG
                     GLSGAALERAVTLTTPVAGYDERRRYWQATLPDVPAAELEEITRRFQVPGGYIRQMGP
                     IATTQAALQQHETVQVEDVREASRSLNRQLLDSLAARLDAGGTWDSLVVSTATERKLH
                     ELEQRCRYRERLLDQLGPGFATTGNRGVRALFSGPSGTGKTLATRILASVIGMDLYRV
                     DLGAVVNKYIGETEKNLHRVLSRAEELDVILLLDEGDALLGTRTEVKSANDRYANLET
                     NYLLQRLEQYQGIVIVTTNAGQYIDNAFQRRMDIVVNFVPPQAQERRAIWDLHLPNET
                     ALPDADLAEIAARCALTGGQIRNAALHATLLAAASGDGEVTRWQVEEAIQSEYRKAGA
                     LSPLTDNGRDDEQHGGLDSFLKSLGVETA"
BASE COUNT          561 a         1469 c         1402 g          780 t
ORIGIN      
        1 tccttgatct cccagtgctt gtccggctga ccatcgaccg gcaacccgcc ccggaccgac
       61 tcgaccgtga cctcgtaact cttgcgggcc gtctcgaggt ccggcacgtt ggcgactttt
      121 ttcttcgtag cggcgtcgat cagcttctgg atgcgctcgt tcttctcgga gcggatgttg
      181 cgaccggagc tcatgttggc gtcggcttcg agcagccaga gattgtcgat tccctcgccg
      241 ccgccgagct gaagctcgtg cttgtgatcg acctggaagc tgagcgggcg gccggtcttg
      301 tcccaatagg gccgcagcga gcggttcttg atcgtcgtct ggttgccgat gatgtagatc
      361 ggcttgccct tgagctgcag gaagtagatc ggctggccgc tcttcgacag agccggcgcg
      421 ttctcgtcct tgatcttctt cgtcagcttc gaatcgatgc cggagccgcc gcgcgcttcc
      481 ttctcccagg tcgcccgctg ggtgtcctcg cgctcctcgc ccgacgcctt caggacgaac
      541 ggagccttcg tgatcttcga cttgaaggtc ggcacccgca tcagcgggat gaccatcttc
      601 ttgctgccga ccttgagctt gccggccttg tcctcgaagt cccacggccc gccgggcgca
      661 ccggtaccgc cgcccggggc cggcttgatc ccatcgcgct ggacgaccgg tgtgttcggg
      721 cttgagctgc ctgcccctgc cgctggcggt ggcgccgctc cgtccccaat cgtcgatgcc
      781 gtcgccaccg atcgctgaac gcgcgtatcc ggcacggcag tcgtcgcgtc gagcgtcgcg
      841 gcagccggtt cggcgactgc cgcctgcgct gccggctggc gttggatcat ctgccgctgg
      901 acaccggccc cggcgtcctg ctggacgacg tgggttgcct cgtgtgccat caggcccaga
      961 tcgcggtccg tttcgccgct gccgagccag atgtcgcgcc cgtgggtgaa agcccgcgcg
     1021 ttcagctcgc gggacgcttc ctgcgactcg gcgtcgtcgt ggacgcgcac gtcgcccaaa
     1081 tcggcatcca ttcgcgattc cagcgtcttg cgggtgctgt cgttcatcgg ccggccggag
     1141 ccggcctgcc caatcgcccg gtcagcagcc ggcgtcgtcg agccggtctg gccggcggcg
     1201 ctggttggcg tcgcggttcc cgccttctcc ggcgcggtct gaaccgactc cgactccgcc
     1261 tcgcgatcct ccggcgtcgt cgtttcggcg cgctggatcg cccgtggatc aggattcttt
     1321 tctggcggct tgttcgtctc ttccttctgg acagccgctt ccggcttctt ctcctccggc
     1381 ttggacgtct tcgccgtttc ccgctgcgcc agcttctgct ccggctcctt cttctccggt
     1441 tcggggatgc cggccttctg gaccggcgtc tgggcgggct tcttctcttc cggcttagct
     1501 tccttctggg ccggtgtcgg ctcgggcttc ttcttctctg gaccggtagc cgtctcacgg
     1561 gccagcgcgc cggccgttac cggcgagata ctgggcggtg ccagctgcga gccggaggtg
     1621 acgtggttcg caaccgcgtt ggcctgctgc tcgtaggcgt cgcccggctg accgacggcc
     1681 agcttcgtct gggccggcaa cgccagccca ccgaccggcc cagccgcgta gtgtggcccg
     1741 agcgcagccg aacggggtgc gtggccggcg ctcgaacggg ccggcgcggg tgcgtgggtc
     1801 gccgccggcc gggctttggc tctcgccttt ggtcgtgcgt gcgcggcgct catcgtctgc
     1861 cgtcctgttc tgccgcccgt ggcgtcgctg gtcgcgtgtc aggccgtttc cacaccgagt
     1921 gatttgagga acgagtcaag tcctccgtgc tgctcatcgt cgcgcccgtt gtcggtcagc
     1981 gggctgagcg ccccggcctt gcggtactcg ctctggatcg cctcctcgac ctgccagcgg
     2041 gtcacctccc catcgccgga ggcggccgcc agcagcgtcg cgtgaagggc cgcgttgcga
     2101 atctgaccgc ccgtcagcgc gcagcgggcc gcgatctcgg ccaggtcagc gtccgggagg
     2161 gccgtctcgt tcggcaggtg cagatcccag atcgcgcgcc gttcctgagc ctggggcggc
     2221 acgaagttga cgacgatgtc catccggcgc tggaaggcgt tgtcgatgta ctgaccggcg
     2281 ttggtcgtca cgatcacgat cccctggtac tgctccagcc gttgcagcag gtagttcgtc
     2341 tccaggttgg cgtagcgatc gttggcggat ttcacctcgg tccgcgtgcc gagcagcgcg
     2401 tcgccctcat cgagcagcag aatgacatcc agctcctcag cccgtgagag gacacgatgc
     2461 agattcttct cggtctcgcc gatgtatttg ttgacgacgg cgccgaggtc gactcggtac
     2521 agatccatcc cgatcaccga ggcgaggatg cgggtcgcca gcgtcttgcc ggtcccgctc
     2581 ggtccgctga agagcgcccg gacgccgcga ttgcccgtcg tggcgaagcc ggggccgagc
     2641 tggtccagca ggcgctcgcg gtagcggcag cgctgctcca gctcgtgcag cttgcgctcg
     2701 gtggccgtgc tgacgacgag gctgtcccac gtcccaccgg catccagccg ggccgccagc
     2761 gagtcgagca gctggcgatt gagcgagcgc gacgcctcgc gaacatcctc gacctggacc
     2821 gtctcgtgct gctggagcgc cgcctgggtt gtggcgatcg gccccatctg gcggatgtag
     2881 ccacccggca cctggaagcg gcgtgtgatc tcttccagct cggccgccgg gacatccggc
     2941 aacgtcgcct gccagtagcg ccgccgctcg tcgtagccgg ccaccggcgt cgtcagcgtc
     3001 acagcccgtt cgagcgccgc gccgctcagg ccaccctcgg tccccatcag cacgccaacc
     3061 ggcccgctgt agccggtcaa cgcaggcagc tcaaccgtct cacccggtcc gaggtcgtag
     3121 ccgatgaccg gcagcaaccg cgccgccgcg cagaacgggc cgagataggg ccagtgcggc
     3181 tcggcgctct gcggctgggc tgccgtgaga tcgacgtagg cgacgccatg tcccattgcc
     3241 cgggcgacag cgcccagcac ctgaagccgg tcagaaccct gcatgccccg gacgacgacg
     3301 gccgtcaccc gcccgttctc gatcaacgcc ggcaactggc gcaactgggc gacgaacgag
     3361 tcggggaaga tcaactcgct gatgcgggga aaatcactgt cgtcgatcgt ccgcagccac
     3421 ggcgcgggag tagccgacgc ctcgcctcgg atcgcgtccc agatcagtgg tggcacccgc
     3481 agcacccact cggaccgggg cgcatcgcgg ttgatggcct cgacgatgcc ggcctcgacc
     3541 agcggccggc agatcgacca ggcatgcagc ccgctgccgg acgtgcgctc ctggacggcc
     3601 tgaccgatca gctccagccc cggccggcgc tcgctgagtg ggcgctggat gtcgctgaac
     3661 agcgtcccga agcgggagtc ctcctcgacc agcccgacca tgacggccgc gatccggctc
     3721 tcgaacgaga gaccggcgtg cttcgcgagg gcgaccagcg gcagatgcgc gtctgcagca
     3781 gcctcccact ccttcagttc cacctcccac cactcgcggg tggcgtccca ggagaggtcg
     3841 tcgggcatgt aggcgaacat cgcctcgaag tagcgcttga ggaacgggta gcgctccagc
     3901 atctggcgca ggttgtcgcc actggtcggg ccgaggtggc gcaggtagta gacgaggtca
     3961 tagagcgcga cgtagacgtt cagccggaag tggttccgag ccgtgtcggg catatcggcg
     4021 aaggcgctct gcatgctccc ggccctcctc aggtgaagtg gatcttcacg acgcggccga
     4081 agtcggggag ccagccggga tcgcgatcca gaccagcgat gcggatggga atcgagatag
     4141 catcgagcgg cagtacgaca tcgacgtggg tggccgaaat atacacctgc cccggctggc
     4201 gcaatagctc cg
//
LOCUS       DASUGJ010000094         8006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_290558, whole genome shotgun sequence.
ACCESSION   DASUGJ010000094 DASUGJ010000000
VERSION     DASUGJ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290558"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFV93_02080"
     CDS             <1..513
                     /locus_tag="VFV93_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5164158.1"
                     /translation="GIVGLGGIGKRVAQLCSALGMRVVAWTRDPSPERLRQAGATYLD
                     LHDLLATADVVSLHLAHAPETDRILSSARLALMKPSAFLVNTARAELVDDDALIAALR
                     AGRLAGAALDVFPEEPLAPDSPWRSLPNVILTPHVGFRTPEATRRSLSIALANLAGAL
                     AGEPRNAVDP"
     gene            complement(560..3175)
                     /locus_tag="VFV93_02085"
     CDS             complement(560..3175)
                     /locus_tag="VFV93_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5164159.1"
                     /translation="MARISRVLTIGLIVVLAFAGVATLVQPRAAIAKGGPHGKVPDQY
                     IVRLKPDVDSASKLDKLKRGGNVSSRHLYRHAFNGFAVRMSADKAEALRADPDVLSVV
                     PDFYISATAETVPSGIQRTDADLNAFTNINREAAGVTVDAGVAIVDTGIDLDHPDLNV
                     VNGVNCAIDGTSPDDLNGHGTHVAGTVGALDNDLGVVGIAPGVRLYAVRVLSAAGEGE
                     FSNAICGLEWVLDHAGEIDVVNMSFGGEAPEGDCNDGSLHEVVCDVIAAGISVVVAAG
                     NGGSDMLGDDAAGFAPANFDEVITVSAIVDTDGQPGGDGAGNFFWGPDDSIARFSNWG
                     ADVDIAAPGVDIYSTWPNGYQASDGTSMASPHVAGAAALVHAMNAGISPGEVRDEILA
                     VAWPQASPEGFSGDSDGFAEPLLNVGALGGDPLLPLPPPPPPPSGCTIDTQSGPPGTI
                     ASLACAGFNPGELASITWDTPSGTFLGLFFTDSAGSGSASPRIPSTSPGAHNIIVIGN
                     SSGTLATIPFTVVSSVKVTPDPLTAGKSAQIRLAGFLAGEQVAITWTDGTASVALPVI
                     TAGSNGTASKAVTVPFTPQGTYTVIATGATSGEVASVFVTVAPSLVASPTSARVGSKI
                     TLTLSAFMPDEQVTINWYDADVVTPIGSITASSVGSATISFNAPETIGGDHLVEAVGD
                     QGTVVARNVKITPRVQVSPTGIKVGEPMTVDLTGYGPNEVVTIDWYTTTSEYVQIGAV
                     TVGPNGSGTLTTPVPDATYGSHRVRGASATSGPNIYATASIRANVVLSPVTGSSGDQV
                     TATATGFKAGESVSIRWYRTASAYVDLTSAPVSATGSGTVTFMVPDSQTLGNHKVIGY
                     GGTSRLQAITTFTVQ"
     gene            complement(3389..4483)
                     /locus_tag="VFV93_02090"
     CDS             complement(3389..4483)
                     /locus_tag="VFV93_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX5164160.1"
                     /translation="MTAYRADIVGSMLRPSWLLEAREQHRAGEISDAEFKRVEDRAVD
                     ECIALQEAAGVDVIADGEMRRNVFASQLVQAAEGFEVVSGNTVDWFRLDGSIDTSPVT
                     VGLTGKIRMKRNLSAEEFVYLRARTDKPTKMTIPSPTMYAYYWVPGISDAAYSSTDAY
                     MADVTDILRNEVDQLVALGGTYIQVDAPEFGMLLDPHQQAWFASKGFDPDRMFHDGID
                     MINAVIDGHPGITFGLHVCRGNDAGRYMARGSYAALASEVFRRTHAERLLLEYDDERS
                     GDFSPLADVPDDKIVVLGLITTKRAAMESADDLVARITEASRYVPLDRLALSPQCGFA
                     SVATGNPVPWEIQAPKLQLVADVARRVWND"
     gene            4579..5457
                     /locus_tag="VFV93_02095"
     CDS             4579..5457
                     /locus_tag="VFV93_02095"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5164161.1"
                     /translation="MSDRLHGILPIVYTPFDANGTIDEDDVRRLVNYLLEAGAHGLAA
                     VGDASECHKLSQGEREWLVEVVIDEAAGRVPVIVGVSAADTSDSVTLARHAERFGAVA
                     VFATPPLGGKATEPILRSHYGTLARAVEIPIVVQDASIPVSSSNVIHLRDEFPNICYV
                     KEESPGDSGHRITELKIRLGDRIGVLSGGSYLLDDLARGADGAIPGSVGVADLARAYD
                     LFRAGDLAGARAAYDHFLPVAHWRRQFPILGAKEILRRIGVFKAAYLRKQEDVWLDEH
                     DHRELSALMERMGPPF"
     gene            complement(5465..5929)
                     /locus_tag="VFV93_02100"
     CDS             complement(5465..5929)
                     /locus_tag="VFV93_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164162.1"
                     /translation="MVGASGQGYRESLARVAEHYRDAGAEVVVDEFLQARSPNYRDIL
                     ERALPGSLAQALTDAGTAFEHEVPGLIDWQIDEAEARRIHQPVLSVLGGESETLWPRF
                     GETHRWLLAHLSDVDGCILPGTTHLLQIEDPRGMAEALVAFWSRHPIPASAA"
     gene            complement(5987..6292)
                     /locus_tag="VFV93_02105"
     CDS             complement(5987..6292)
                     /locus_tag="VFV93_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5164163.1"
                     /translation="MATAKIDGAELEYEVAGAGEPVVCIHGAFIADTFRPLLDQPTLA
                     GRHQTLHYHRRGYAGSSRTSGIVSINQQAADCRAVLRQLGINRAHVVGHSSGGSIVL"
     gene            complement(6447..7415)
                     /locus_tag="VFV93_02110"
     CDS             complement(6447..7415)
                     /locus_tag="VFV93_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5164164.1"
                     /translation="MPSAQRPLTRRTLLRAASGLTGLVALSLAGCAGDDDPPRQVVTS
                     TDAYPNPDLLASVSWLADRIDDPNLRLLDCSPIETWRKGHLPHASHVWWQDTIEINNN
                     TYGMLTGAPMRDEIVRETGITPETTVVCYDDRGGQYAARLLWMLHVQSFTNVRLLDGG
                     RQGWRGAGHDLSDDSASPPAGAIEAVQNESVIAHGNDIAARLNDAGYVILDTRTPDER
                     AETWFDHLRRGAIPGSRYLSRTDFLTPDGAALVAPDALRARLTAAGVPADAPEIVVYG
                     LHGTLACLPYVALRALGYPSVRVYDGSWAEWGANPAWPVEPLSDGS"
     gene            7662..>8006
                     /locus_tag="VFV93_02115"
     CDS             7662..>8006
                     /locus_tag="VFV93_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164165.1"
                     /translation="MIPRLASLKTLFLAAVLILGIGMLAGCSIGNSGDDDDTGSAAST
                     PAATIQAPQGQETSNVAASPTRTADSQPTDVSKTASDSSTSDAMKVLDTNNDGKVDRV
                     ELFSLADLVEQVN"
BASE COUNT         1394 a         2534 c         2673 g         1405 t
ORIGIN      
        1 ggcatcgtcg ggctgggcgg gatcggcaaa cgggtcgcgc aactttgtag cgcgctgggg
       61 atgagggtcg tggcctggac gcgcgacccc tcgcccgagc ggctccggca ggccggcgca
      121 acctatctgg acctgcacga cctgctggcg actgccgatg tcgtctcgct ccacctggcg
      181 catgcgccgg agactgacag aatcctttcg tcggcgcggc tggccctgat gaaaccgtcc
      241 gccttcctcg tcaacacggc ccgcgccgaa ctggtggatg acgacgcgct catcgcagcc
      301 ctgcgcgcag gcaggctggc tggtgccgcg ctggatgtct ttcccgagga accgcttgcg
      361 cccgacagcc cctggcgatc gctcccgaac gtgattctca ccccacacgt cggcttccgc
      421 acgcccgaag ccactcgccg ctcactgtcg atcgcgctcg ccaacctggc cggcgcgctg
      481 gctggtgagc cgcgcaatgc cgtggatccc tgagtgaagc cgatcgatcg gctacgtttc
      541 gccgcttacc cgcagcagac tactgcacag tgaaggttgt tattgcctgg agccggctcg
      601 tcccgccata cccgatgacc ttgtgattgc ccagtgtctg gctgtccggc accataaacg
      661 tgacggttcc gctgccggtg gctgacacag gtgccgatgt caagtcaaca taggctgagg
      721 ccgtccgata ccaccgaatc gagacacttt cgcctgcctt gaaccccgtg gcggtggctg
      781 tgacctggtc tccggatgag ccggtgaccg gcgacagaac gacgttagct cgaatcgaag
      841 cggtggcgta gatattcggg ccactcgttg cgcttgcgcc ccgaacccgg tgtgacccgt
      901 atgtggcatc cggcaccggc gtggtgagcg ttcccgagcc gttcggccca accgtgacag
      961 cgccgatctg gacgtactcg ctggtcgtgg tgtaccagtc gatcgtgacg acctcgttcg
     1021 gtccatagcc ggtcaggtca accgtcatcg gttcgccaac tttgatgcct gtcggtgaca
     1081 cctgcactct gggcgtgatc ttgacgttgc gagccacgac cgttccctgg tctccgactg
     1141 cctccacgag atggtcacct ccgatggttt cgggggcatt gaacgaaatc gtggcgctcc
     1201 caaccgagga cgccgtaatc gagccgattg gcgtgacgac atcggcgtcg taccaattga
     1261 tcgtgacttg ctcatccggc atgaaggcgc tcaacgtgag tgtgattttc gagccgacac
     1321 gggctgacgt tggggaagcg accagcgatg gcgcgacggt aacgaagacc gacgcaacct
     1381 cacccgatgt cgcgccggtc gcgatgactg tgtaggtacc ctgtggagtg aacggcacgg
     1441 tcacggcttt ggacgcggtt ccgttcgaac cggccgtgat taccggcagg gcgacggacg
     1501 ccgttccgtc cgtccacgtg atcgccacct gctcgccggc caggaatccg gcgagccgga
     1561 tctgcgcact cttgccggct gtcagcggat cgggtgtgac cttgacgctg gagacgacgg
     1621 tgaacgggat cgtggctagc gtgccgctgg agttgccgat gacgatgatg ttgtgagcgc
     1681 ccggtgacgt cgatggaatg cgtggcgatg cggatccgga gccggctgag tcggtgaaaa
     1741 acagacccag aaatgtgccg gatggggtat cccaggtgat gctcgccagt tcgcctggat
     1801 tgaagccggc acatgcgagg ctggcaatcg tgcctggcgg gccggattgc gtgtcgatcg
     1861 tgcagccgga tggaggcggt ggaggcggtg gtagtggcag caacggatca ccaccgagcg
     1921 cgccaacgtt caacagcggc tccgcgaacc catccgaatc gccgctgaac ccctctggcg
     1981 atgcctgggg ccaggcgact gccagaatct cgtccctgac ctcgcccggc gatatccccg
     2041 cgttcatcgc gtgaaccagc gcggccgccc cggcaacgtg cggtgacgcc atgctggtcc
     2101 catccgacgc ctggtacccg tttggccagg tggagtagat gtcgacccct ggcgcggcga
     2161 tatcaacgtc tgcgccccaa ttgctgaacc gggcaatgga atcgtctggc ccccagaaga
     2221 agtttcccgc gccatcgccg ccaggctggc cgtccgtatc gacgatggca gagacggtga
     2281 tgacctcatc gaagttggcc ggcgcgaagc cggcagcgtc gtctcccagc atgtcggagc
     2341 caccattgcc ggcggcgaca acgacagaga ttccggcggc gatgacatcg cagacgacct
     2401 cgtgcaggct gccgtcattg cagtcaccct caggtgcttc gccgccgaag ctcatattga
     2461 cgacgtcgat ctcgccggcg tggtcgagaa cccattccag gccgcagatc gcgttcgaga
     2521 actcgccttc ccccgccgca ctcagtacgc gcaccgcgta gagtcgaacg cccggcgcga
     2581 tgccaacgac accgagatcg ttgtcgagtg cgccaacggt gccggccaca tgggtaccgt
     2641 ggccgttcag atcgtcgggg ctcgtgccgt cgatggcgca attgacaccg ttgacgacgt
     2701 tcaggtcagg atgatcgagg tcgataccgg tatcaacgat cgcgactccc gcatcgaccg
     2761 tcacgccggc cgcctcgcgg ttgatgttcg tgaaggcgtt taaatccgcg tcggtgcgtt
     2821 ggataccgct tggaaccgtt tccgccgtcg cgctgatgta gaagtccggc acgaccgaga
     2881 gcacatccgg gtctgcgcgc aacgcctctg ccttgtcagc gctcatccgg actgcgaaac
     2941 cgttgaacgc gtgccggtac aggtgcctgg acgagacgtt cccgccgcgc ttgagcttgt
     3001 cgagcttgga cgcagagtcg acgtcaggct tgagccgaac gatgtactga tccggcacct
     3061 tgccgtgtgg cccgcccttg gcgatggccg cccgcggctg aaccagcgtc gccactcccg
     3121 cgaaggcaag gacaacgatc agtccgatcg tcaggactcg agagatgcgt gccatctgaa
     3181 ccccttgtcg gctgcgacga accgtccgga acaacatccg ggcagcggca gagttcacca
     3241 gattatgact ttggcgctat catcccacgg agctatgcac gtggatagca gtcgctggtc
     3301 ctgtttctgg aggcacctgt acgtacagtc aataggggcg gctcgactcg ccgaaccgat
     3361 tggctcgaac ggcgggacgc ctgcgacctc aatcattcca gacgcggcgg gcgacgtcgg
     3421 cgacgagttg gagcttgggg gcctggatct cccaggggac agggttgccg gtcgcgacgg
     3481 aggcgaagcc gcattgcgga gagagggcaa gccggtcgag cgggacgtag cggctggcct
     3541 cggtgatgcg ggcgaccagg tcgtcggcgc tctccatcgc agcccgcttg gtcgtgatca
     3601 ggccgaggac gacgatcttg tcgtcgggca cgtcggccag cggcgagaag tcgccggaac
     3661 gctcgtcgtc gtactccagc agcaaccgct cggcatgcgt gcgacggaag acctcggatg
     3721 cgagcgcggc gtacgagccg cgcgccatgt agcggccggc gtcgtttccg cggcagacgt
     3781 gcaggccgaa ggtgatgcca gggtgaccgt cgatgacggc gttgatcatg tcgataccgt
     3841 cgtggaacat ccggtcggga tcgaacccct tgctcgcgaa ccaggcctgc tggtggggat
     3901 cgagcaacat cccgaactcg ggcgcgtcga cctggatgta ggtcccgccg agcgcgacga
     3961 gctggtcgac ctcgttacgc aggatgtcgg tcacgtcggc catgtaggcg tcggtcgagg
     4021 agtaggcagc gtcggagatg cccggcaccc agtagtaggc atacatcgtc gggctgggga
     4081 tcgtcatctt ggtcggcttg tcggtccggg cacgcaggta gacgaactcc tcggccgaga
     4141 ggttgcgctt catgcggatc ttgccggtca gcccgaccgt gacagggctg gtgtcgatgc
     4201 tgccgtcgag ccggaaccag tcgaccgtgt tgccggagac gacctcgaac ccctcggcgg
     4261 cctggacgag ctggctggcg aagacgttgc gtcgcatctc accgtcagcg atgacatcga
     4321 cgccggccgc ctcctgaagg gcgatgcact cgtcaacggc gcgatcctca acccgcttga
     4381 actcagcgtc gctgatctct ccggcgcggt gctgctcacg cgcctccagc aaccacgacg
     4441 gccgtagcat gctgcccacg atatctgcac gataggctgt cattcggcgt atgctccttt
     4501 cgtctagatg aaactcagca ctgtcaaagc ttccaggtaa gcctcatggt aaccgcagat
     4561 cgcgtgggga ggaacgtcat gtcagatcgg ctgcacggca ttctgccgat cgtctacaca
     4621 ccattcgacg ccaacgggac gatcgacgag gacgatgtac ggcggctggt gaactacctg
     4681 ctggaggccg gggcgcacgg gctggctgcg gtcggcgacg ccagcgagtg tcacaagctc
     4741 agccagggcg agcgggaatg gctggttgag gtcgtgatcg atgaagcggc agggcgcgtg
     4801 ccggtcatcg tcggcgtctc agcggctgac acgagtgata gtgtgacgct ggcgcgccat
     4861 gcggagcgat tcggggccgt cgccgtcttc gcgacaccgc cgctcggcgg aaaggccacc
     4921 gagccgattc tgcgcagcca ttacggcacc ctcgcgaggg cagttgaaat cccaattgtt
     4981 gttcaggacg ccagcatacc ggtttcttca tcgaatgtga ttcacctgcg cgacgagttc
     5041 ccgaacatct gctacgtgaa ggaagagtca ccgggcgact ccggccatcg catcaccgaa
     5101 ctgaagattc ggctcggaga ccggatcggc gtgctctcag gtggatcgta cctgcttgac
     5161 gatttggcgc gtggggcgga tggagcgatt cccggcagcg tgggagtcgc cgatctcgcg
     5221 cgcgcctacg atctgtttcg agccggcgac ctggccggag cgcgcgccgc ctacgaccac
     5281 ttcctgccgg tcgcacactg gcggcggcag ttcccgatcc tcggcgccaa ggagatcctg
     5341 cggaggatcg gcgtcttcaa ggcggcgtat ctgcgcaaac aggaagacgt ctggctggat
     5401 gagcacgacc accgcgagtt atccgccctg atggagcgga tggggccgcc gttctagtgc
     5461 ctgttcacgc agccgatgcc ggtatcggat ggcgcgacca gaacgcgacg agcgcctcgg
     5521 ccatgccgcg tggatcctca atctgcagta ggtgcgtcgt gcctggcaga atgcagccgt
     5581 cgacgtcgga caggtgcgcc agcagccacc gatgcgtctc tccgaagcgg ggccagagcg
     5641 tctcactctc gccaccgagg acggacaaca ccggctggtg gattcgccgc gcttctgcct
     5701 catcaatctg ccagtcgatc agtcccggca cctcatgttc gaacgcagtc ccggcgtcgg
     5761 ttagcgcctg agcgaggcta ccaggcagcg ctcgttcgag gatgtcgcga tagttcggcg
     5821 atcgcgcctg gagaaactca tcgacgacaa cctccgcgcc ggcgtcacgg tagtgctcgg
     5881 cgacccgcgc cagtgactcg cgatatccct gcccactcgc gccaaccatc agcgccggtt
     5941 cgagcagggc cagcgattgg accagatccg gagcatcaag cgccagttac aggacgatcg
     6001 agccgccaga cgaatgtccg acgacgtgcg cccggttgat tcccagctgg cgcagcacgg
     6061 ctcgacaatc ggcggcctgc tggttgatgc tgacgatacc ggacgtgcga ctgctgccgg
     6121 cgtatccacg gcgatggtaa tgaagcgttt gatgccggcc ggccagcgtc ggttggtcta
     6181 gcaacggccg gaacgtgtcc gcgatgaatg ccccatggat acagacgacc ggttcgcctg
     6241 cgccagcaac ctcgtactca agctctgccc cgtcgatctt cgccgtcgcc actgctgtct
     6301 ccttgtcaag atgccatgct tgaatgctcg cgacacgcat cgacgtcagc cgtgatgcgt
     6361 atgcagtccg tggctggtca acgtgcgact cacgcacatc gcgcttacgc ctgcaacgat
     6421 cgtacggtca ggccatcaat agactgtcaa gacccgtccg atagcggctc gaccggccag
     6481 gccgggttcg cgccccactc ggcccaggag ccgtcgtaga cgcggacgct ggggtagccg
     6541 agggcgcgca gggcgacgta ggggaggcag gcgagcgtgc cgtgcaggcc gtagacgacg
     6601 atctcgggcg cgtcggcggg gacgccggcg gcagtcagcc tggcgcgcag ggcgtcgggc
     6661 gcgacgagtg cggcgccgtc gggggtcagg aagtcggtgc gggacaggta gcggctgccg
     6721 gggatcgcgc cacggcggag gtggtcgaac caggtttcgg cgcgttcgtc cggggtgcgg
     6781 gtgtcgagga tgacgtagcc ggcgtcattg aggcgggcgg cgatgtcgtt gccgtgggcg
     6841 atgaccgact cgttctggac cgcttcgatc gcgccggctg gcgggctggc gctgtcgtcg
     6901 ctcaggtcgt ggccggcccc tcgccagccc tggcgaccgc cgtcgagcag ccgaacgttc
     6961 gtgaagctct ggacgtgcag catccagagc aggcgggcgg cgtactggcc gccacggtcg
     7021 tcgtagcaga cgacggtcgt ctcgggcgtg atgccggtct cgcggacgat ctcgtcgcgc
     7081 atcggcgctc cggtcagcat cccgtaggtg ttgttgttga tctcgatcgt gtcctgccac
     7141 cagacgtgtg acgcgtgtgg gagatggcct ttgcgccagg tctcgatcgg cgagcaatcc
     7201 agcagccgca ggttcgggtc gtcgatccga tcggcgagcc agctgacgct cgcgaggagg
     7261 tcaggattcg ggtaggcgtc ggtcgaggtg acgacctggc ggggagggtc gtcgtcgccc
     7321 gcgcaaccgg ccagcgagag ggcgaccagc ccggtcaggc cggaggcggc gcgcagcagc
     7381 gtccggcgcg tcagtggccg ttgggccgat ggcatacgcg atgcaacaac agcgatagac
     7441 cggcgaacgt tgctgggaga ccggggcgcg tggcgatagc gcggcgcact cacgttttga
     7501 ttcagacaac tttcaggttt gcttcacgcg aagctcatat cgtccgttca tactggatac
     7561 acaccggagg acccggccca gtggcggatg cgatcatgcc acagccgcgg ctgactcgtt
     7621 gcggtcgagc cgtccagact cgggaaaggc aagcaggacc catgataccc agactcgctt
     7681 ctttgaagac cctctttctc gcggcggtac tgatcctcgg gatcgggatg ctggccggtt
     7741 gctcgatcgg caacagcggc gatgacgacg ataccggctc ggcggcatcc accccggccg
     7801 caacaataca ggcgccgcag ggtcaggaaa catccaacgt ggcagcatcg ccaaccagga
     7861 cggccgatag ccagccgacc gacgtctcca agaccgccag cgacagcagc accagtgacg
     7921 caatgaaggt tctcgacaca aacaacgacg gcaaggtcga ccgcgtcgag ctgttctcgc
     7981 tggccgatct ggtcgagcaa gtcaac
//
LOCUS       DASUGJ010000095         4485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_152024, whole genome shotgun sequence.
ACCESSION   DASUGJ010000095 DASUGJ010000000
VERSION     DASUGJ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152024"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFV93_02120"
     CDS             complement(<1..394)
                     /locus_tag="VFV93_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164166.1"
                     /translation="MRIIVNHLTRMVPGFICVAGISVETNRHIRPICNRVMLPLRWAA
                     SEGGVFGLGNIVDLGQVRHRGERPEVEDREFREQHLRCIDRLPADDFWSLLDEHHETR
                     LQRIFGPELTLVGQSCTTDQGAGRASLGV"
     gene            complement(404..841)
                     /locus_tag="VFV93_02125"
     CDS             complement(404..841)
                     /locus_tag="VFV93_02125"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX5164167.1"
                     /translation="MEIYTIGFTQKSARQFFDLLKANGIRRLIDIRLSNTGQLAGFTK
                     STDLAYFLEEICGAEYTHQPLLAPTKQLLTDYRNGRTSWAGYERIFESLMTERQIETA
                     ISPSLFNVPSVMLCSEPTADQCHRRLVAEYLQRHWDDVTIHHL"
     gene            complement(781..1410)
                     /locus_tag="VFV93_02130"
     CDS             complement(781..1410)
                     /locus_tag="VFV93_02130"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX5164168.1"
                     /translation="MNCTSQPVAGQVVYTVGHSNQPAEEFLDLLRQHEIEVVVDVRSS
                     PFSRFVPQFNKRELQATVREAGFQYVYLGRELGGRPDPGSDLIDDEGHAIYGLIAETN
                     AFNDGLRRLLEGIGRYRVALMCSEEDPTDCHRRLLVARVLILDHGIDVRHIRGDGRID
                     TEDDLTRMPYDAPIQQSLFGSTDDDARKRAWRSIRSVLPRNLRDSSSTS"
     gene            complement(1562..2065)
                     /locus_tag="VFV93_02135"
     CDS             complement(1562..2065)
                     /locus_tag="VFV93_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164169.1"
                     /translation="MATSYDVSGVLASGGEFHATPSMIEVTYPGTDKPPVAIDLGDVS
                     GVRRRGTEVTLIRGGRETTLRTTELAAAGRLESLVRHYHETRNAEEFEHQLIRLYSQG
                     YQIVALTPKIAQLRKPKKFNFLLAAILALLAVLPALIYVFYYFSQPEQTALVILEDSG
                     EVVVRQV"
     gene            complement(2143..3171)
                     /locus_tag="VFV93_02140"
     CDS             complement(2143..3171)
                     /locus_tag="VFV93_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5164170.1"
                     /translation="MSTGRRGLRVAPFGGEHVEPAARLLADRHQRDRLRAPSLPAAFE
                     TVGATLPMVQQAFLSAETSGVVAYLGEAFAGFLLGRRTVIPAESTIAPYYRPSSILVR
                     YDAHAALSGSTWDIYRELFAAAADRWVSDGLTAHYIQLPAGDDAAIDAWASVGFGRDM
                     GWGLRDTGPIEPAQMIPGLTIRRTTVDDLDVTFQLDTALARYESRSPVFMPYPTAVAE
                     REWRADLSASLADDQLSFWLAELDSRPVGLLNVNPPPAHISPLLTPDTMVNISAASVV
                     PDVRGIGIGGALVRHALDAARSEGHAWCRMSWMTANLYSSRFWSRHGFEPVAWRMSRV
                     IDERAVAW"
     gene            complement(3191..4399)
                     /locus_tag="VFV93_02145"
     CDS             complement(3191..4399)
                     /locus_tag="VFV93_02145"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5164171.1"
                     /translation="MSDNTAQTNTGPLAGIRVVDLTRVLSGPYCTMMLADMGADVIKI
                     EQPGKGDDTRAWGPPWVDGPEGARESAYYLSINRNKRGITLDLKSDFGKEVLWKLIDG
                     ADVLVENFSPGTAGRLGFGPDEVLARRPSLIYASVSGFGQTGPSSQRPAYDLILQGMG
                     GVMSVSGPPGHPTKFGVPIADIASGMFSAYAVACALVARKETGKGQVIDTSMLGGQVA
                     LLSYQAAIFFATQQTPPTTWNAHPIVAPYQTFPSSDGFVNIAVGNDRLWAAFTTAFGM
                     EDVAADERFKDNAGRITNIAALAEIIERVFSDFTTAEIVSKLDAAGVPSGPIYTVPQV
                     FADPQVQHAGLERRIQHPTLGEISVTGFPYKLSETDATLRMPPPLHGQHQAEILRELG
                     YTDEEIAERA"
BASE COUNT          781 a         1381 c         1520 g          803 t
ORIGIN      
        1 tcacgccaag tgacgcgcgg ccggcgcctt ggtccgtcgt gcacgattga ccaaccagcg
       61 tgagttcagg gccgaaaatt cgctgcaggc gggtttcgtg atgttcgtcc agcagcgacc
      121 agaagtcatc ggccggtaac cggtcgatgc agcgcagatg ctgttcgcga aactcacggt
      181 cctcaacctc tgggcgctcg ccgcgatggc ggacctgacc aagatcaacg atgttcccaa
      241 gtccgaagac gccaccttcg gacgcggccc agcgcaacgg caacatgaca cggttgcaga
      301 tcggtcgtat gtgccggttc gtttcgacgc ttatgccggc cacacagatg aatccgggaa
      361 ccatgcgcgt cagatggttg acgatgatcc gcaccggccc acctcacaaa tggtgaatcg
      421 taacgtcgtc ccagtggcgc tggagatatt cggcgacgag tcgccgatga cactggtcgg
      481 cggttggttc gctgcagagc atgacagatg gcacgttgaa gagggatggt gagattgccg
      541 tctcaatctg gcgttcggtc atgagcgatt caaagatgcg ttcgtagccg gcccaactgg
      601 tgcggccgtt gcggtagtca gtgaggagtt gcttggtggg cgccagcagc ggttggtggg
      661 tgtattcggc gccgcaaatc tcttccagaa agtaggccag gtcggtggac ttggtgaagc
      721 cggcgagctg gccggtattg ctgaggcgga tgtcgatcag gcggcgaatg ccgttggcct
      781 tcaggaggtc gaagaactgt ctcgcagatt tctgggtaaa accgatcgta tagatctcca
      841 cgcccgcttc ctcgcgtcgt catcggtgct gccgaacaga gattgctgta tgggcgcatc
      901 atacggcatc cgcgtcaaat cgtcttcagt gtcgatccgg ccatcgccgc gaatgtggcg
      961 cacgtcaatg ccatgatcga ggatcaggac gcgcgcgacg agcaggcggc ggtggcagtc
     1021 ggtcgggtcc tcctcgctgc acatcagggc gaccctgtag cggccgatgc cttcgagcag
     1081 ccggcgcaga ccgtcgttga aggcgttcgt ctcggcgatc aggccgtaga tggcgtggcc
     1141 ctcgtcgtcg atcagatcgc tgccggggtc ggggcgaccg ccaagctcac gcccgaggta
     1201 gacgtactgg aagcccgcct cacggaccgt ggcctgtagc tcgcgcttgt tgaattgcgg
     1261 cacgaagcgc gagaacgggg acgagcgcac atcgacgaca acctcgatct cgtgctggcg
     1321 tagcaggtcg aggaattcct cggccggctg gttggagtgg ccgacggtgt agacaacctg
     1381 gccggcgacg ggctgcgacg tacagttcat ctcagcgaaa ctaccctact tcctggaacg
     1441 tcgttcaccc gacaagtagg ggccggtctt gtgccggccc tgttccatac ccgaggcgtt
     1501 cgggcacccg agcagggcgg gccaagaccg gcccctacat gctgacaggg cgagtgtcat
     1561 ctcacacctg ccggacgacg acttcgccgc tgtcttcgag gatgacgagg gcggtttgtt
     1621 cgggttgaga gaagtagtag aagacgtaga tgagggccgg gagaacggcc agcagcgcga
     1681 gaatggcagc gagtaagaag ttgaacttct tcggcttgcg gagctgggcg atcttcggcg
     1741 tcagcgcgac gatctggtag ccctggctgt agaggcggat gagctggtgc tcgaactcct
     1801 cagcgttgcg cgtctcgtga tagtggcgga cgagcgattc gaggcggccg gccgccgcaa
     1861 gctcggtcgt gcgcagcgtc gtctcccggc cgccgcggat gagggtcact tcggtgccgc
     1921 gccggcggac gccgctgacg tcgccgaggt cgatcgcgac cggcggcttg tcggtcccgg
     1981 gataggtgac ttcgatcatg ctgggtgtgg cgtggaactc gccgccgctc gccagcaccc
     2041 cggatacgtc gtaactcgtc gccatctgtc cgcctcttgt cgcttcactc gatgtcgtgg
     2101 cccagcggcc ggattcgtcg tgattcgtga tggtactgga aatcaccatg ccacggcgcg
     2161 ctcgtcgatc acgcgagaca ttcgccaggc gaccggctca aagccatgcc gcgaccagaa
     2221 acgcgatgag tagaggttgg ccgtcatcca cgacattctg caccacgcat gcccttcgga
     2281 tcgcgcggcg tcaagcgcgt ggcgaacgag cgcgccgcct atgccgattc cgcgaacgtc
     2341 cggcacaacc gacgcggcag agatgttgac catcgtgtcc ggcgtcaaca gcggggagat
     2401 gtgcgctggt ggcggattaa cgttcagcag gcccaccggg cggctatcca gctccgccag
     2461 ccagaaggat aactgatcgt cggcgagcga cgcgctgaga tcggcgcgcc actcccgctc
     2521 agcaactgcg gttggatacg gcatgaagac gggggatcgc gactcatagc gggcaagggc
     2581 cgtgtcgagc tggaaagtaa cgtcgagatc gtccaccgtc gtgcgccgga tcgtgaggcc
     2641 gggaatcatc tgagcaggct cgattgggcc cgtgtcacgc agcccccagc ccatgtcacg
     2701 tccgaatccg accgatgccc aggcgtcgat ggctgcgtca tcacctgccg ggagctggat
     2761 gtagtgagct gtcagcccgt ccgaaaccca gcgatctgcg gctgccgcaa acagttcccg
     2821 gtagatgtcc caggtgctgc cagagagggc ggcatgcgca tcgtaacgga cgaggatgct
     2881 cgacgggcga tagtaaggcg cgatcgtact ttctgctgga atcacggttc gtcgtcccag
     2941 cagaaacccg gcgaacgctt cgccaagata cgcgacgacg ccactcgttt cggcagagag
     3001 gaacgcctgc tggaccatcg ggagtgttgc cccgacggtc tcgaacgcgg ccggcagcga
     3061 cggcgcccgg agtcgatcgc gctggtgacg atcggccagc agccgcgccg ccggctcgac
     3121 gtgctcgccg ccaaacgggg cgactcgtag cccacgccgg ccggtactca tggcatctcc
     3181 gcgttacgca tcacgcccgc tccgcgatct cctcatccgt gtagccaagc tcgcgcagga
     3241 tctcggcctg gtgctggccg tggagtggtg gcggcatccg cagcgtggcg tcggtttccg
     3301 agagcttata ggggaagccc gtcacggaaa tctcgcccag cgtcgggtgc tggatgcgcc
     3361 gttccagtcc ggcgtgctgc acctgcgggt cggcgaagac ctgcgggacg gtgtagatcg
     3421 ggccgctcgg gacaccggcc gcgtccagct tgctgacaat ttcggcggtt gtgaagtcgc
     3481 tgaagacgcg ctcgatgatc tcggccaggg cggcgatgtt ggtgatccgg ccggcattgt
     3541 ccttgaagcg ttcgtcggcg gccacatctt ccatcccgaa ggcggtcgtg aaagcggccc
     3601 agagccggtc gttgccgacg gcgatgttga cgaagccgtc cgagctgggg aacgtctggt
     3661 agggcgcgac gatcgggtgg gcgttccagg tcgtcggcgg cgtctgctgg gtcgcgaaga
     3721 agatggcggc ctgatacgag agcagcgcga cctgaccgcc cagcatcgag gtgtcgatca
     3781 cctgcccctt gccggtttcc ttccgagcga ccagcgcgca ggcgaccgcg taggcgctga
     3841 acatgccact ggcgatgtca gcgatgggaa cgccgaactt ggtcgggtgc cccggcggac
     3901 cgctgacgct catcacgccg cccatccctt gcaggatcag gtcgtaggcc gggcgctgac
     3961 tggacgggcc ggtctggccg aagccggaga cggaggcgta gatcagcgag ggcctgcgcg
     4021 ccagcacctc gtccgggccg aagccgagcc gaccggccgt gccaggcgag aagttctcga
     4081 ccagcacgtc ggcgccgtcg atcagcttcc agagcacttc cttgccgaag tcactcttga
     4141 ggtcgagcgt gatgccgcgt ttgttgcggt tgatcgacag gtagtaggcc gattcgcggg
     4201 caccctccgg cccatcgacc cagggcggcc cccaggcacg ggtgtcgtcg cccttgccgg
     4261 gctgctcgat cttgatgaca tcggcgccca tgtcggccag catcatcgtg cagtacggcc
     4321 cggacagcac ccgcgtcaga tcgacgacgc ggatgccggc cagcgggccg gtgttcgttt
     4381 gtgcggtgtt gtcggacatc gcttttccac ccccgaaaat cgcttccgcc agtgagagtc
     4441 atcgctcccc gcgtaggggc gacggccgca gcctcgccct gcttg
//
LOCUS       DASUGJ010000096         4169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_847514, whole genome shotgun sequence.
ACCESSION   DASUGJ010000096 DASUGJ010000000
VERSION     DASUGJ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847514"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..2356)
                     /locus_tag="VFV93_02150"
     CDS             complement(56..2356)
                     /locus_tag="VFV93_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164172.1"
                     /translation="MAGPRRATRCYRVAVVLSLLLLPLTVAGVAAAKPNGDSPGQAKQ
                     AEAGSWVGKLRGDSALVAIVYDGAQLVAYVCDDGRVGSWFFAAPGPGSSFQLAGAGDS
                     LLEVTLGSVAKGQYSKDGQVYEFNAHKSKDEVLFRADTAPNDIAVVGGWIQDGGQTRG
                     TVAIGSSLIAAPKLAPTVKITLSPDVLATLSPAAMTPDTLSRAIANTTKFVWAAAGDS
                     YASGEGNPERGISDPDDPEDFSGLRWGNDPSITIPIPGVSLGADLTTCHRSDEAAAPK
                     AQRFLEDTYTGMTIRLGFVACGGAETGALTGTYDGPDTSEESLLGYDRVNEQAQLDRI
                     AGFATDEGELNALYMSIGGNNAGFGEIITDCVSPFGPSNCADVWDTVLDARLFNLVKP
                     GGEYEQVNDAIVERFGDDLPVLIQAYPNPLDDGSTGIPPVCFGTDYDAHGEVGFGGYD
                     DALQDNVTHEEADWAYDVPDKLNSAVESAAGTYGWTVVDGHLAAFKGHGICTAEPFDN
                     LNSAGLHRQGRDIPNTGPFLFSSGLMHPNDDGYTQIAQAAIDDLRPLVDNVARADLVK
                     PLNVRVADATKNGSITLRWNDRSKSENAYEVLVLPVRPQDGGVLIRPAGSTPFGAGYR
                     IRLSGSDLQQYIAQFAGGGQFSFQVRACQTGIRFVEGAGAELQCGEWSAPLIGTNVAP
                     ATPTAVQHSTQTVRTGTRFMIVDVVTWSPQADAIEFVVRIETPDGSASETRTTGTRFQ
                     RSVLAGATYKVAACNRIGCSFYVTAP"
     gene            2697..3380
                     /locus_tag="VFV93_02155"
     CDS             2697..3380
                     /locus_tag="VFV93_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164173.1"
                     /translation="MSHHISERARRLLTPALIVALVLAAGTTIVASGNGEDDTYAGCL
                     TAGGALNKVVVNPTEDQTCPGNQTLIVWNEKGQQGDPGPQGAQGPQGPQGAQGIQGEP
                     GPAGPQGNTGPQGPAGPAGDPGPAGPAGAQGPAGLSGFEIVTEFSNDDGDGFVSTHTA
                     TCPTGKAAIAGGWRFFGGGSDNLIVVTNRPDISSDGEQSWEVVVADITPSDAAYSIEV
                     YATCAVVSP"
BASE COUNT          721 a         1334 c         1405 g          709 t
ORIGIN      
        1 gctgccgggt gccctctggg cggtggacga cagggggact gcgcgagtgc ccttatcagg
       61 gagccgttac atagaacgag cagccgatcc gattgcaggc cgccaccttg taggttgcgc
      121 ctgccagcac cgatcgctgg aagcgtgtcc cggtcgtgcg ggtttcgctg gcgctgccat
      181 cgggggtctc gatccggacg acgaactcga tcgcgtcggc ctgcggcgac cacgtcacga
      241 catctacgat catgaagcga gtaccggtcc gtactgtttg tgtactgtgc tggaccgctg
      301 tcggggttgc cggggcgaca ttcgtcccga tcaggggcgc cgaccattcg ccacactgga
      361 gctccgcacc ggcgccttcg acgaagcgga tgccggtctg gcaggcgcgc acctggaacg
      421 agaactgccc gccaccggcg aactgcgcga tgtattgctg caggtccgag ccggagagcc
      481 ggatgcggta gccggctccg aacggtgtcg agccagccgg ccggatcagc acgccgccat
      541 cctggggacg caccgggagg acgaggacct cgtaggcgtt ctccgacttc gaacggtcgt
      601 tccagcgcag cgtgatcgag ccgttcttcg tcgcatcggc aacacgcacg ttgagcggtt
      661 tgacgagatc ggcccgtgcg acgttgtcga ccagtggccg caggtcgtcg atggcggcct
      721 gcgcgatctg ggtgtagccg tcgtcgttcg ggtgcatgag gccgctgctg aacaggaagg
      781 ggcccgtgtt cgggatgtca cggccctggc ggtgcagccc ggccgagttc aggttgtcga
      841 acggctcggc ggtgcagata ccgtgacctt tgaaggccgc caggtggccg tcgacgacgg
      901 tccagccgta ggtgccggcg gcagactcaa ccgctgagtt gagcttgtcc ggaacgtcgt
      961 aggcccagtc cgcctcttca tgggtcacgt tgtcctggag cgcgtcgtcg tagccgccga
     1021 agcccacctc tccgtgcgcg tcgtagtcgg tgccgaagca gacgggtggt atgcccgttg
     1081 agccgtcgtc gagcgggttc gggtaggcct ggatcagcac cggcaggtcg tcgccgaagc
     1141 gttcgacgat tgcgtcgttg acctgctcgt attctccacc cggcttgacc aggttgaaca
     1201 accgggcgtc gaggacggtg tcccagacgt cagcgcagtt ggaggggccg aacgggctga
     1261 cgcagtcggt gatgatctcg ccgaagccgg cgttgttgcc gccgatcgac atgtagaggg
     1321 cgttgagctc gccttcgtcc gtggcgaagc ccgcgatccg gtcaagctgc gcttgctcgt
     1381 tgacccgatc gtagccgagc agcgattcct ccgacgtgtc cggtccgtcg tacgtgcccg
     1441 tcagcgcgcc cgtctccgcg ccgccgcagg cgacgaaccc cagccggatc gtcatcccgg
     1501 tataggtgtc ctccaggaag cgctgcgcct ttggcgcggc cgcctcgtcg ctgcggtggc
     1561 aggtggtgag gtcggcgccg aggctgacac cggggattgg gatcgtgatc gaggggtcat
     1621 taccccagcg caggccgctg aagtcctcgg ggtcgtccgg atcggagata ccgcgctctg
     1681 gattgccctc gccggaagcg taagagtcgc cggcggcggc ccagacgaac ttcgtcgtgt
     1741 tggcgatcgc ccgtgagaga gtgtccggcg tcatcgccgc ggggctgagc gtcgccagca
     1801 cgtcggggga gagggtgatc ttcaccgtcg gcgccagctt cggggccgcg atcagcgatg
     1861 acccgatcgc cacagtcccc cgcgtctgac cgccgtcctg gatccagcca ccgacgacgg
     1921 cgatgtcgtt cggcgcagta tcagcgcgga acagcacctc gtccttcgac ttgtgggcgt
     1981 tgaactcgta gacctgccca tctttcgagt actgtccctt ggcgacggag ccgagggtca
     2041 cctccagcag cgagtcaccg gcgccggcca gctggaagct ggaaccggga ccgggggcgg
     2101 cgaagaacca cgaaccaacg cgtccgtcgt cgcagacgta ggcaacgagc tgggccccgt
     2161 catagacgat ggcgaccagc gcggaatcgc cgcgcagctt cccaacccag ctacccgcct
     2221 cagcctgctt cgcctggccg ggcgaatccc cattcggctt ggccgctgcg acgccggcca
     2281 ccgtcagcgg cagcaacagg agcgagagca ctacggccac gcgatagcaa cgcgtggccc
     2341 tgcgtggacc tgccatcgat tccctcctcg catgtcgatg aacctcatca ccaacaagga
     2401 acggccaggc ggtggcatgt tcgtgacgtc atcgctacgg ccggacatgg atgatgaaat
     2461 gggcttctga ttgcagaccg tagtcgggcg gcgtaaatgc ggcattaatc aggcgcttgg
     2521 gggatgctgt cgtgccgcag atcgaggtca cctggtcgac gcgcggcagg ccgacgcccc
     2581 ggttaatgct ccattaatgg tcaggtcgct accttgcttg caggcaggta gcgcaaccgc
     2641 accgaccggc atgttcgacg agccggcggt ccggcacgta cgcgaaggag caaccgatgt
     2701 ctcatcacat cagcgagcgc gcccggcgcc tgctgacacc ggctctcatc gtcgccctcg
     2761 tgctggcggc cggcacgacg atcgtggcat caggcaatgg cgaggacgac acctacgccg
     2821 gctgcctgac cgccggcggc gccctcaaca aggtcgtcgt caatccgacc gaggatcaga
     2881 cctgtccggg caaccagacc ctcatcgtct ggaacgagaa gggccagcag ggcgatccgg
     2941 ggccgcaggg agcacaggga ccacagggtc ctcaaggggc tcagggcatt cagggtgagc
     3001 cgggcccagc ggggccgcag ggcaacactg gtccgcaggg gccggctggg ccagccggag
     3061 atccaggccc agcgggaccg gcgggtgcgc agggaccagc gggattgagc gggttcgaaa
     3121 tcgtcacgga gttctcgaac gacgacggag atgggtttgt gtccacgcat accgcgacgt
     3181 gtcccaccgg gaaggcagcc atcgctggcg gctggcggtt ctttggtgga ggcagtgaca
     3241 acctcattgt cgtcacgaat cggcccgata tcagcagcga cggcgaacaa tcctgggagg
     3301 tcgtcgttgc ggacatcact ccaagcgacg cagcctacag catcgaggtg tacgccacct
     3361 gcgcggtcgt ctctccgtga ggcgcgcgcc aatcaattga tcgtgggagt gccgaacgtg
     3421 cccggataat gaatgtgacc agtcgttacg aatccgtcag gtgccgtggt caccggcaat
     3481 ccgacaatga ccccggcgac ggcgccgcct gacgacaacc gacactccag cgagacgggg
     3541 gcgctttcgc gcccccgttg ttgtgcccgc cggagcgggg ctggctggac accaccgcgg
     3601 ccggggcggt cgcgacgaga gcgaagcatc agcgcgatgc caacagtacg agacgaacgt
     3661 cctgttgtcg cggcacgaat gtcgtgggtg acgtgcgaac gtacgcggtg atacactgat
     3721 caccgacaac gagtcacaat cgcgtcacag tagccgcttt cgggacgttc ggggggattc
     3781 aatcccgcgc ctgtcggcta caatcggaga tagcgacaga gtcaacacgt ccagccggtg
     3841 ctgatgtcgc ccctgctcgc cgacgagcag gtcgcggtcg gtgaacgacc tttcatccgg
     3901 cactccggct gctcgaaacg tgacgcgcgg tcaccgtatg ctagactccc aatcacttga
     3961 cgatccgcga gcgagttctg gcaaccagtg accaccccgt ccgcagcccg gagacagttc
     4021 ctcgtcgtgc gcggcagcgt cagcacgtgt cctgaccagt gacaggacca gtgaggatgg
     4081 tttcaacccc atgagtcggt acgatttgta cgcagtccgt tatccgctcc ttccactcaa
     4141 gaacgtcgtc atttttcccc gtaatgtcg
//
LOCUS       DASUGJ010000097         8636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1542520, whole genome shotgun sequence.
ACCESSION   DASUGJ010000097 DASUGJ010000000
VERSION     DASUGJ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542520"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1337)
                     /locus_tag="VFV93_02160"
     CDS             complement(138..1337)
                     /locus_tag="VFV93_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5164174.1"
                     /translation="MPITTLTAARLFDGTGNAPIENAFLRIEDGKIKEVGRKEDLGSA
                     AAGARDFGDATLLPGLVNMHTHLTCSASLSVLHDALHDSYETKLMRAVENARASLLSG
                     VTTIRDCGTLNSIVFAIREASKQNLITAPHVWASGEVLTSTAGHCYFFGVECDTIDEA
                     RHAVRRQVKAGADFVKVMATGGNLTPNSNPNREQFSGEDMIAIVEDSARLGRYVAAHC
                     HGTPGIYNAVAANVRTIEHCSFVIPGDVEYREDAAAGVAEKGIYVVPTISTGERNRQR
                     QIDAGTMSPKQLETRHKRLENLRRLNAAGVRFVSGNDAGMALTRFDDFQLDLELLVEH
                     VGFTAAEAIRTATGQAAEAIGSDEFGTLEAGRRADVLAVRGNALNDIGALRNIQMIMK
                     SGQVVVG"
     gene            complement(1453..2589)
                     /locus_tag="VFV93_02165"
     CDS             complement(1453..2589)
                     /locus_tag="VFV93_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5164175.1"
                     /translation="MVDTVEGLRTQQTSIYDRIGVRTIINASGATTAVGGTLMPPEVT
                     AAMAEAAQAFVVVEELNAAVGEVIARVTGAEAGYVTAGSAAGMLLAAAACITGTDRAK
                     VKRLPQTNGMANELVIHRCQRINYDQMFRAAGATLVEIGLPRSTERWELEAAIGERTA
                     AVVYIDSPSTTPGAIDFATVVEVAHSRGVPVIVDAASTLPPIWHLQRWISWAADLVIY
                     SGGKGIRGPQDSGLLAGRKDLIAAAAMNGAPNAAIGRGMKVSKEAMAGLWVALERFMQ
                     HDHEGDFAQHLQQAEYLRANIQERSDARCELVADQSAHPAPILTVYPTTGSWNAREVL
                     QALKDGTPSIHCKVAEGGLEINTHCLLPDEPEQILDRLFAVLDA"
     gene            complement(2606..3439)
                     /locus_tag="VFV93_02170"
     CDS             complement(2606..3439)
                     /locus_tag="VFV93_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5164176.1"
                     /translation="MVSDASVFGAGQREQHGRTVHWQEMWRHELLAALERDPVVIVPV
                     GSVEQHGPHCPMDVDISHTQAMAVETARAIDDFPVIVAPPVWIGLTHYNMGEVGTITA
                     SVETWIALVSEICRSIWANGFHRIILLNGHGGNRDIQRVVSIKLAEEDIWVLPITHWE
                     MAWDVLHNEAETDAGFIGHGGEWETSLQLYLRPTLIDRERMVADPERPGFSPEIQAFT
                     GFPERRREREHGVHGNPLSASAEKGEHLFNAVRDMLIEVCRQYHQQPVRNYREFGSHC
                     P"
     gene            complement(3533..4735)
                     /locus_tag="VFV93_02175"
     CDS             complement(3533..4735)
                     /locus_tag="VFV93_02175"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5164177.1"
                     /translation="MGIYDTLGVQPVINAAGPVTRLSGAPLHPEVAAAMAEAAQHCFR
                     IEDLQAAAGRYLAEVTGAEAGYVTAGAAAGLSLSAAAAIAGLDVAAMDRLPDTTGLRD
                     EIVVQRAHLTAYTHALRLAGAKLVEVGYLGFPGQGITWPWQVEAAVNERTAALAWSVG
                     SAQGVVPLEEMVAIGRRHRLPVIVDAAASLPPVENLQRFIAAGADLVAFSGGKAIGGP
                     QASGILVGRADLIESVALQHQDMDVYPETWTWRERYIASGRLPGPPHHGIGRPLKVGK
                     EEIAGLVVALRRFLVRDHLAERREQEERLAAIIAGLDGIPGVESELLDDSRAPRPYPT
                     LVIRIDETIVEQPVETIVNALADGDPPICVSQNFLHERALGIVATTLKPGDEATIAGR
                     LREVLRGA"
     gene            4914..6107
                     /locus_tag="VFV93_02180"
     CDS             4914..6107
                     /locus_tag="VFV93_02180"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEX5164178.1"
                     /translation="MSRYDLVVRGGRVIDPAQDLDAVCDVAILGSRVAAIGHDLADEG
                     VRNGVVDAAGLIVTPGMVDLHTHVYWGVPPLGIEPDPHCLARGVTTAVDAGSAGAATF
                     PAFRRYVIDVSATRIVAMLHISSIGMARDDRDGGVGELEDIRWARVDRAIDIARSHAD
                     VIKGIKVRLQSEMVGEDPDQCREALRRTQQAAHAIGKPVMVHVGGTTIGLDEILGALN
                     TGDIVTHCYHARGEGVLDENGKIRPSVRRAIERGINFDVGHGRGSFDYGVARAALDQG
                     LLPGTISSDIHAWNIAGPVYDLATTASKFLHLGVSLNDVIRRVTINPAMAIGMDDTIG
                     TLSPGSAADLSLLRLTDGEWPFMDSTGNVEIGRQRLEPVSVIRAGRQYPCTPAVYATS
                     HMHSH"
     gene            6125..6421
                     /locus_tag="VFV93_02185"
     CDS             6125..6421
                     /locus_tag="VFV93_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164179.1"
                     /translation="MDDLAVASREELASFYRDVTDSNARAMLDFLIDHAGERFEARAI
                     MEALQFAQHRDVALAAHAVGEAAARHGIKRPWLEAQLGYTMSPEMAAALREARS"
     gene            complement(6418..7266)
                     /locus_tag="VFV93_02190"
     CDS             complement(6418..7266)
                     /locus_tag="VFV93_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5164180.1"
                     /translation="MRYPHPVRFSIVCGQRAPWPELVARIREAEELGFDVFNVVDHFY
                     GVHNVMDPTHEAWTALGGLAMASERIRLGVMVCGNTYRNPAFLLKQAITVDHISNGRV
                     ELGVGAGWLEREHEAYSFDFPSARERVDRFEEALEIWDLVQQQERTNYDGRYYQLVDA
                     PFEPKAIQQPRLPVLIGASLPRMLGLTARHADIWNARGTPDEARAANDLLDEKCGEVG
                     RDPSQIMRSISNGLHLWDSPEAFSSAITAFKDAGFTDFQIPWPRTAEAHAVMRQVATE
                     MMPTLR"
     gene            complement(7470..>8636)
                     /locus_tag="VFV93_02195"
     CDS             complement(7470..>8636)
                     /locus_tag="VFV93_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEX5164181.1"
                     /translation="VGAVDGPRVRIAGRWVLNFTSSNYLGLSHDPEVRERTAATVRRW
                     GSSLAMPRLLATDSLTAQLERKLAGLVHAEAALLFPSTLHAAHDVLSLLAGDAGLIAI
                     DERAYPISLAAARSVAWPNGEMRRFQHDDPESLARLLAERGQRDAVVICDGVYPANGA
                     PASVRAIGRVAASAGATVYVDDAHGLGVLGAHPGPAQPYGYGGGGTLRYSGAQQGNIA
                     YVSSLSKALGVPLAFAAGPARFIEYLRLTSASVTHSSPPCQLVVAAALATLDRQVACG
                     DALRRRLLYNVRHFRAEANRLGLPLTSNSLFPIQSIAFASPAAAIASGRRLRLHDIWP
                     VLALRPEDHPTGATLRFVITALHTKEDIDEAIDALARTAACQGRFDDPALEWTG"
BASE COUNT         1315 a         2910 c         2920 g         1491 t
ORIGIN      
        1 tgaaactcga tcaatccaat caatccggga ggggcgaatt cgcaaaatcg ccctgctcac
       61 tcgcccgaac gcctcggtgg ggttgccggg cgattttgcg aaatcgcccc taccggattg
      121 attggattga tcgcgattca gcccacgacg acctggccgg acttcatgat catctggatg
      181 ttgcgcaggg cgccgatgtc gttgagcgcg ttgccccgga cggcgaggac atcggcgcgg
      241 cggccggctt cgagggtgcc gaactcgtcg gatccgatcg cttcggcggc ctggccggtg
      301 gcggtccgga tcgcctcggc cgctgtgaag ccgacgtgct cgaccagcag ctccaggtcg
      361 agctggaagt cgtcgaagcg cgtcagggcc attccggcgt cgttgccgga gacgaaacgg
      421 acgccggccg cgttgaggcg gcgcaggttc tccagccgct tgtggcgcgt ctccagctgc
      481 ttcggcgaca ttgtcccggc gtcaatctgg cgctggcggt tgcgctcgcc ggtgctgatc
      541 gtcgggacga cgtagatgcc cttctcggcg acaccggcgg cggcgtcctc cctgtactcg
      601 acgtcgccgg ggatgacgaa cgagcagtgc tcgatcgtcc ggacgttggc ggcgacggcg
      661 ttgtagatgc cgggcgtccc gtggcagtgg gcggccacgt agcgacccag ccgggcggag
      721 tcttcgacga tcgcgatcat gtcctcgccc gagaactgct cgcggttggg attgctgttc
      781 ggcgtcaggt tgccgccggt cgccatcact ttgacaaagt cggccccagc cttgacctgc
      841 cggcgaacgg cgtggcgtgc ctcgtcgatt gtgtcgcact cgacaccgaa gaagtagcag
      901 tgcccggccg tcgaggtcaa cacctcgccg ctggcccaga cgtgcggggc ggtgatcagg
      961 ttctgcttcg acgcctcgcg gatcgcgaag acgatcgagt tgagcgtccc gcagtcgcgg
     1021 atggtcgtga cgccggagag cagcgaggcc cgcgcgttct cgacggcccg catcagcttc
     1081 gtctcgtacg aatcatgcaa cgcgtcgtgc aacacggaaa ggctggcgct acaggtcaga
     1141 tgcgtgtgca tgttgaccag ccccggcagc agcgtcgcgt cgccaaagtc ccgcgccccg
     1201 gccgccgccg accccagatc ctccttccgc ccaacctcct tgatcttccc atcctcaatc
     1261 cgcaggaacg cgttctcaat cggcgcgttc cccgtcccgt cgaacaagcg ggcggcggtt
     1321 aatgtggtga ttggcattgg tttctcccct cgttggcttg tgggtcggga tccgggattc
     1381 gggggtatct gacatccgtg tcctgttcga cacccccgaa tcccgaatcc cgattcacga
     1441 atcccgaccg catcacgcat ccaacaccgc gaacagccgg tccaggatct gctccggctc
     1501 gtcgggtagc aggcagtggg tgttgatttc cagcccgccc tcggccacct tgcagtggat
     1561 cgacggcgtg ccgtccttga gcgcctggag aacctcgcgc gcgttccagc tgccggtcgt
     1621 cgggtagacg gtcaggatcg gcgccgggtg ggcggactgg tcggcaacca gctcgcagcg
     1681 ggcgtccgag cgctcctgaa tattggcgcg caggtactcg gcctgttgca ggtgctgggc
     1741 gaagtcgcct tcgtggtcgt gctgcatgaa ccgttcgagc gccacccaga ggccggccat
     1801 cgcctccttg ctgaccttca tcccccggcc gatggcggcg ttcggcgcgc cgttcatcgc
     1861 tgccgccgcg atcagatcct tccggccggc cagcagcccg ctgtcctgcg gcccgcgaat
     1921 gcccttcccg ccgctgtaga tgacgaggtc ggcggcccag ctgatccagc gctgcaggtg
     1981 ccagatcggc ggcagtgtcg aggcggcgtc gacgatgacc ggcacaccgc gcgagtgggc
     2041 gacctcaacg acggtcgcga agtcgatcgc gccgggcgtc gtcgagggcg agtcgatgta
     2101 gacgacggcg gctgtccgtt cgccaatggc ggcctccagc tcccagcgct cggtcgagcg
     2161 gggcaggccg atctcgacca gcgttgcccc ggccgcccgg aacatctggt cgtagttgat
     2221 gcgctggcag cggtggatga cgagctcgtt cgccatcccg ttcgtctgcg gcaagcgctt
     2281 gaccttcgcc ctgtctgtcc cggtgatgca ggctgccgct gccagcagca tcccggccgc
     2341 cgagccggcc gtcacgtagc ccgcctcggc gccagtcacc cgcgcgatga cctcaccgac
     2401 cgcggcgttg agttcctcga caacaacgaa cgcctgggcg gcctcggcca tcgcggccgt
     2461 tacctccggt ggcatcagcg tcccgccgac ggcagtcgtc gcgccgctgg cgttgatgat
     2521 cgtccggacg ccgatccggt cgtagatgct ggtctgttgc gttcgcagtc cctcgactgt
     2581 gtcaaccaag tcggccctcc cttcgctacg gacagtgcga cccgaactcg cgatagttgc
     2641 gcaccggctg ctggtggtac tgccggcaaa cctcgatcag catgtcgcgg acggcattaa
     2701 agagatgctc gcccttctcg gccgaggctg agagcgggtt gccgtggacg ccgtgttccc
     2761 gctcgcgccg ccgttcaggg aagccggtaa acgcctggat ctccgggctg aagccgggcc
     2821 gctccggatc ggccaccatc cgctcgcggt cgatcagtgt tggccgcagg tagagctgga
     2881 gcgatgtctc ccattcgccg ccgtggccga tgaagccggc atcggtctcg gcctcgttgt
     2941 ggagaacatc ccaggccatc tcccagtggg tgatcggcag gacccagata tcctcctcgg
     3001 ccagcttgat cgagacgacg cgctggatgt cacggttacc gccgtggccg ttgaggagga
     3061 tgatacggtg gaagccgttg gcccagatcg agcggcagat ctccgagacg agcgcgatcc
     3121 aggtctcgac gctggcggtg atcgttccaa cctcgcccat gttgtagtgc gtcagcccga
     3181 tccagaccgg cggcgcgacg atgaccggaa agtcgtcgat cgcccgcgcc gtctcgaccg
     3241 ccatcgcctg ggtgtggctg atatcgacat ccatcggaca gtgcggtcca tgctgctcaa
     3301 ccgacccgac cggcacgatc acgactgggt cacgctccag tgccgccagc agctcgtgac
     3361 gccacatctc ctgccagtgg accgtcctgc cgtgctgctc ccgctgcccc gccccgaaga
     3421 ctgatgcgtc agaaaccatt gtgttccctt tcgtgatgtg tgatgtgtga tgcgtgttgc
     3481 gtgatgcggg gcccgcggcc gccactccgc gtaccgcgta ccgcgtaccg cgttacgcac
     3541 ctcgcaacac ttcgcgcaag cgcccggcga tcgtcgcctc gtcgccaggc ttgagcgtcg
     3601 tcgcgacgat gccgagcgcc cgttcgtgga ggaagttctg cgagacgcag atcggcgggt
     3661 cgccatccgc gagcgcgttg acgatcgtct cgaccggctg ctcgacgatc gtttcgtcga
     3721 tccggatgac gagtgttggg tacgggcgcg gcgcgcggct gtcgtccagc agttccgact
     3781 cgacgccggg gatgccgtcg agcccggcga tgatcgcggc gaggcgctcc tcctgctcgc
     3841 gccgctcggc gagatgatcg cgcaccagga accggcgcag tgcgacgacg aggccggcga
     3901 tctcctcctt gccgaccttc agcggcctgc cgatgccgtg atggggcggg ccgggtagcc
     3961 gcccgctggc gatgtaacgc tcgcgccagg tccacgtctc cgggtagaca tccatgtcct
     4021 ggtgctggag ggcgaccgac tcgatcaggt cggcccggcc aaccaggatg ccggacgcct
     4081 gcggcccgcc gatcgctttt ccgccgctga aggccactag gtcggccccg gccgcgatga
     4141 agcgctgcag gttctcgacc ggcggcagcg acgcggcggc atcgacgatg accggcagcc
     4201 ggtgcctgcg cccgatcgcg accatctctt cgagcgggac gacgccctgc gcgctgccga
     4261 ccgaccaggc cagcgcggcc gttcgctcgt tgacagccgc ctcgacctgc cacggccatg
     4321 tgatgccctg accggggaat ccgaggtagc cgacctcgac cagcttcgcc cccgccagcc
     4381 gcagcgcgtg ggtgtaggcc gtcaggtgtg cccgctggac gacgatctcg tcgcgcaggc
     4441 cggttgtgtc cggcagccga tccatcgccg cgacgtccag cccggcgatg gcggctgccg
     4501 ccgacaggct caacccggca gctgcgccgg ccgtcacgta gcccgcctcg gcgccggtga
     4561 cctcggccag atagcgcccg gccgccgcct gcaagtcctc gatccggaaa cagtgctggg
     4621 ctgcctcggc catcgcggcg gctacttccg gatggagcgg cgcgccgctc agccgcgtca
     4681 ccggcccggc agcgttgatc accggctgga ccccgagtgt gtcatagatg cccatcgcgc
     4741 cccctcatgg tatctggcct cggttggtgg cgctatgcta aacgacgttt cctcaatgtg
     4801 cgctctgaac tcgtgccaag gttggagcac ctcttggcca cggatggcgt ccgtgacata
     4861 ctcccccggt cacttccgat ctgtcacgct gcccaatccg gaggaggaaa ctgatgtcac
     4921 gctacgatct cgtcgtccgt ggcggtcggg tgatcgatcc cgcgcaggac ctcgacgcgg
     4981 tctgcgacgt ggcgatcctg gggagccggg tggcggcgat cggtcacgat ctggcggacg
     5041 agggggttcg caatggtgtg gtagatgcgg ccgggctgat cgtgacgccg gggatggtcg
     5101 atctgcacac gcatgtctac tggggcgtgc cgccgctcgg gattgagcca gacccacatt
     5161 gcctggcacg cggcgtgacg acagccgttg atgccggctc ggccggggcc gcgacgttcc
     5221 cggccttccg tcgctacgtg atcgatgtct cggccacgcg aatcgtcgcg atgttgcata
     5281 tctccagcat cgggatggcg cgcgacgacc gcgacggtgg tgtcggcgag ctggaggaca
     5341 ttcgctgggc gcgggtcgac cgcgcgatcg acatcgcccg cagccacgcg gatgtcatca
     5401 agggcatcaa ggtccgcttg cagtccgaga tggtcggcga ggaccccgac cagtgccgcg
     5461 aggcgctgcg ccggacccag caggctgccc acgcgatcgg taagccggtc atggtccacg
     5521 ttggcgggac gacgatcggg ctggatgaga tcctcggcgc gctcaacaca ggcgacatcg
     5581 tcacccactg ttaccatgcg cgcggcgagg gcgtcctcga cgagaacggg aagatccgcc
     5641 catcggtccg gcgggccatc gaacgcggta tcaactttga cgtgggccac ggccgcggca
     5701 gcttcgacta cggcgtcgcc cgcgcggcgc tcgaccaagg cttgctgccc ggcacgatca
     5761 gctccgacat ccacgcctgg aacatcgctg ggccggtcta cgatctggcg acgaccgcca
     5821 gcaagttcct ccatctcggc gtctcgctga acgacgtcat ccgccgcgtg acgatcaatc
     5881 cggccatggc gatcggtatg gacgacacga tcggcacgct ctcaccggga tctgccgccg
     5941 atctcagcct gctgcgtctc acggacggtg agtggccctt tatggactcg accggcaatg
     6001 tcgagattgg ccgccagcgg cttgagccgg ttagcgtgat ccgggccgga cgtcagtacc
     6061 catgcacgcc ggccgtctac gccacgtcac acatgcattc gcattgaact gaaacagggg
     6121 tgtcatggac gatcttgccg tcgccagccg tgaggaactg gccagcttct accgcgatgt
     6181 caccgattcg aacgcccggg cgatgctcga cttcctgatc gaccacgccg gcgagcggtt
     6241 cgaggcacgc gcgatcatgg aggccctgca gttcgcgcag catcgtgacg tggcgctcgc
     6301 ggcccacgct gtcggtgagg ccgcagcgcg gcatggcatc aagcgcccct ggctggaagc
     6361 gcagcttggc tacacgatgt cgccggagat ggcggcagcg ttgcgggagg cgcgcagcta
     6421 gcgcagcgtc ggcatcatct cagttgccac ctgccgcatg acggcgtgcg cctcggcggt
     6481 tcgcggccag gggatctgga agtcggtgaa gccggcatcc ttgaaggcgg tgatcgcgct
     6541 gctgaaggcc tcgggtgagt cccagaggtg caggccgttg ctgatcgagc gcatgatctg
     6601 gctggggtcg cggccgacct cgccgcactt ctcatccagc aggtcgttgg cggcgcgggc
     6661 ttcgtccggc gtgccgcgcg cgttccagat gtcggcgtgc ctggccgtca gccccagcat
     6721 tcgcggcagg ctggcgccga tcaggaccgg tagccggggc tgctggatcg ccttcggctc
     6781 gaacggggcg tcaacgagct ggtagtagcg gccgtcgtag ttggttcgtt cctgttgctg
     6841 gaccagatcc cagatctcca gtgcttcctc gaagcggtcg acccgctcgc gggccgatgg
     6901 gaagtcgaag ctgtaggcct cgtgttcgcg ttccagccag ccggcgccga cgccgagctc
     6961 gacccggccg ttcgagatgt gatcgacggt gatcgcctgc ttgagcagga aggctgggtt
     7021 gcgataggtg ttgccgcaga ccatcacgcc cagccggatt cgctccgacg ccatcgccag
     7081 tccgcccagc gccgtccagg cctcgtgagt cgggtccatg acattgtgga caccgtagaa
     7141 gtggtcgacg acgttgaaga catcgaagcc cagttcctca gcctccctga tccgcgcgac
     7201 cagctccggc cacggcgctc gctggccgca gacgatcgag aaccgaaccg ggtgcgggta
     7261 tcgcatgaca actcctcatt gcggctgtga acttcgttgg tcacatggtg ccacaggttc
     7321 gcggaggtag atagaccaac cgcgcggtta gacagcgttc ccgacgcccc cctgtcgtcc
     7381 tgagcggcag cgaaggacct ctcctcgtta gccgagcatg gcgtcaacgc aggggaggtc
     7441 cttcgctgcc gctcaggacg acagacgggc tacccggtcc attccaacgc tggatcgtcg
     7501 aagcgcccct ggcaggccgc tgttcgcgcc agcgcgtcaa tcgcctcatc gatatcctcc
     7561 ttggtgtgca gtgcggtgat gacgaagcgg agtgtggcgc cggttgggtg atcctcgggt
     7621 cgcagcgcga ggaccggcca gatgtcgtgg aggcgcaacc gccggccgct ggcgatcgct
     7681 gcggctggcg acgcgaaggc gatcgactgg attgggaaga ggctgttgct ggtcaatggc
     7741 aggcccagac ggtttgcctc agcgcggaaa tgccgaacgt tatagagcaa ccggcgtcgc
     7801 aacgcgtcgc cacaggccac ctggcgatcc aacgtcgcca gtgcggcggc gacgaccagc
     7861 tggcagggcg ggctgctgtg cgtcacgctc gctgatgtca gccgcaggta ctcgatgaag
     7921 cgggccgggc cggcggcgaa ggccagcggc acgcccaacg ccttcgacag gctgctgacg
     7981 taggcgatgt tcccctgttg agcgccgctg tagcggagcg tgccgccccc gccatacccg
     8041 tagggttgag ccgggccggg atgcgcgccg agcacaccca gcccgtgcgc gtcatcgaca
     8101 tacaccgtcg cccccgccga ggcggcgacg cgaccgatcg cgcgcacgga tgcgggcgcg
     8161 ccgttagcgg gatagacccc gtcgcagatg acaacggcat cgcgctgacc gcgttcggcc
     8221 aggagccgcg ccagcgattc aggatcgtcg tgctggaagc ggcgcatctc gccgttgggc
     8281 caggcgacgc tgcgtgctgc tgcgaggctg atcggatagg cgcgctcgtc gatcgcgatc
     8341 agccccgcat ctccggccag cagcgacagc acgtcgtggg cggcgtggag ggtggagggg
     8401 aagagcaggg ctgcctcggc atggacgaga ccggccagct tccgttcgag ttgcgccgtc
     8461 aggctgtcag tcgccagcag gcgtggcatc gccagcgagc tgccccagcg ccggacggtc
     8521 gctgctgttc gctctcggac ctccgggtcg tggctcagcc cgaggtagtt gctcgatgtg
     8581 aagttgagca cccaccggcc ggcgatccgg acccgaggtc catcgaccgc cccgac
//
LOCUS       DASUGJ010000098         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1820164, whole genome shotgun sequence.
ACCESSION   DASUGJ010000098 DASUGJ010000000
VERSION     DASUGJ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820164"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..417
                     /locus_tag="VFV93_02200"
     CDS             94..417
                     /locus_tag="VFV93_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5164182.1"
                     /translation="MGPRIRELRIERGMTLDELARAANISASHLSRLERGQTAPSFKV
                     AADIAAHIGVKPSELATIQREQSDVDQALIEALTARGLSDEIAQHICDKISTVARAAL
                     LEIVR"
     gene            complement(529..1323)
                     /locus_tag="VFV93_02205"
     CDS             complement(529..1323)
                     /locus_tag="VFV93_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164183.1"
                     /translation="MSSNEVTTRVERAEFDAFQGLYRAAAATCRTGWLELEGISTVWS
                     AEDDDPGFSCVMNLVNAADPAATLGRIEAQVRANGATVLGVDAPPALAARLPEAELAR
                     LGYAADCQEHIWARQITADESFPAASDPRITPVTPDERETFARVLNIGYEVSENSVRG
                     RIFASTIGLPGWYHYLVRFDGEPGSASVLYVTEGVAQLFVATTMPDFRGRGAQQSLIQ
                     RRLADAQSAGCNLATSQTITDNASPRNMARHGFERLHTRWIYGKVL"
     gene            1463..2128
                     /locus_tag="VFV93_02210"
     CDS             1463..2128
                     /locus_tag="VFV93_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164184.1"
                     /translation="MTIGDGQAFQNWQIALQTTVADAIEHLQVLIFDDGWQELRAAAG
                     SDEAALRESVASYERELARLFGFVRASGALADAVAWSTLKTDFRRAALDANTEISLRT
                     ALETGLYAAELIPLADHAGYRAWATALMGFLASRAATGPTAPGPDAEADDRLQWAYDR
                     LADLEDHTAFNAAFLNWLNAEYPPSTDRDAIVADQQRLLALPRFDIVLNTSLRWLSSQ
                     PPA"
BASE COUNT          387 a          728 c          695 g          342 t
ORIGIN      
        1 tgcctgtgat acgcaaaccg gtcaaatccg ccacgaaggt gcggggccgg gccagtaagg
       61 agaaaccgag tgcccgccaa aacgcggaat tcaatgggcc cacggatccg cgagctgagg
      121 atcgagcgag ggatgacgct cgacgaactg gctcgcgctg ccaacatctc cgccagccat
      181 ctctcgcggc ttgaacgggg tcagacggca ccatcgttca aggtcgcggc cgacatcgcc
      241 gcccacatcg gcgtcaagcc cagcgagctt gccacgatcc agcgcgagca atcggatgtc
      301 gatcaggcgc tgatcgaggc gctgacggcc cgtggcctga gcgacgagat cgcccagcac
      361 atctgcgaca agatctccac cgtcgcccgc gcggcgttgc tggagattgt gcgttaaaca
      421 agtgctgagt gctgagttga aagtgctgat tcatttccgc gtaggggcga tggccgcagc
      481 ctcgcctggc caaccactca gcactttcaa ctcagcactc agcactcgtt acagcacttt
      541 cccgtagatc cagcgcgtgt ggaggcgctc gaagccgtgg cgggccatgt tgcgggggga
      601 ggcgttgtcg gtgatggtct gtgaggttgc caggttgcag ccggcggact gagcgtcggc
      661 caaccggcgc tggatcagcg attgctgggc gccacggccc cggaaatctg gcatcgtcgt
      721 cgcgacgaag agctgcgcga ccccctctgt cacgtacagc accgatgccg aaccgggctc
      781 gccgtcgaag cggacgaggt agtgatacca gcccggcagg ccgatcgtgc tggcgaaaat
      841 ccggccacgc accgagttct ccgacacctc gtagccaatg ttcagcaccc gcgcgaaggt
      901 ctcgcgttca tcgggcgtca cgggcgtgat gcgcggatca ctcgcggccg ggaacgattc
      961 atcagccgtg atctgacgcg cccagatatg ctcctggcag tcggcggcgt agccgagacg
     1021 ggcgagctcc gcctccggca gacgggctgc cagcgccggc ggcgcgtcaa cgccgaggac
     1081 ggtcgccccg ttggcacgca cctgcgcctc gatccgtccc agggttgccg ctgggtcagc
     1141 cgcattaacc aggttcatga cgcaggagaa gccggggtcg tcgtcctccg cagaccagac
     1201 tgtggagatg ccctccaact ccagccagcc ggttcggcag gtcgcagcag cggcccggta
     1261 gaggccctgg aaggcgtcga actcggcccg ctcgacgcgg gtcgtcacct cgttgctgga
     1321 caccgaactg ccatcctccc gctcagcggt cgtcgcgtgc catcggctag cgtgcccatc
     1381 ataaccgccg cgtgccatac tcgggggcca gaagggacat ggccgtggtc gacatcagta
     1441 gcgacagttt tctgacacga aaatgacaat cggagacgga caggcattcc agaattggca
     1501 gatcgcgctt cagacaaccg tcgccgacgc gatcgagcac ctgcaagtgc tgatattcga
     1561 tgacggctgg caggagttac gggcggctgc cggcagcgat gaagcggcgc tgcgcgagag
     1621 tgtcgccagc tacgagcggg aactggcgcg gttgttcggc ttcgtccgcg cctcgggtgc
     1681 gctggcggac gccgtcgcct ggtcgacact gaagaccgac ttccgccggg cagcgctgga
     1741 tgccaacacc gagatcagcc tgcgcaccgc cctggaaacc ggcctctacg ccgccgaatt
     1801 gatcccgctc gccgaccacg cgggctatcg tgcctgggca accgcgctga tgggctttct
     1861 ggcctcccgg gccgccaccg gcccgaccgc gccggggccg gatgccgagg ccgacgatcg
     1921 cctccagtgg gcctacgacc ggctggcgga ccttgaagac cacacggcct tcaacgccgc
     1981 gttcctcaac tggctcaacg ccgaatatcc acccagtacc gatcgcgacg cgatcgtcgc
     2041 tgaccagcag cggttactag ccctgccgcg cttcgacatc gttctcaaca ccagcctgcg
     2101 ctggctgtca tcgcagccgc ccgcgtaggg gcgacggccg cagcctcgcc ct
//
LOCUS       DASUGJ010000099        10194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1542647, whole genome shotgun sequence.
ACCESSION   DASUGJ010000099 DASUGJ010000000
VERSION     DASUGJ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542647"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFV93_02215"
     CDS             complement(<1..291)
                     /locus_tag="VFV93_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164185.1"
                     /translation="MNVLVISTGQPAIEWLALAISIRGHTPLRAADGPAALTRLTEQP
                     CQLVILDEIEGGDDERLELCRQLRALPEMRTTPIWVTAGADDLAGIVMAIDAG"
     gene            complement(380..1423)
                     /locus_tag="VFV93_02220"
     CDS             complement(380..1423)
                     /locus_tag="VFV93_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5164186.1"
                     /translation="MFQGLPWTRLALGLLISAGAIAIVLMRVNLDDVAEAFRTARYDW
                     LLLAIAASFLSVAIRAQRWRLLCWPAQMSFTRAFGILSVGAALTSLLPLRLGDVVRAY
                     LAGEMERRSKAWALTTVVVERVLDVVALLVIIVMLLPFVSLPKWAAESTRITVVLAVA
                     GVSVICGVWFGRAALERRLGPLAGRLARGPLERVRSMVRHVVDGLAILGQPARLATVI
                     LWTAANWLTTGVVLWATLRAFGVEGSPAIAMLLVVVSAASVAVPISPSAVGVYHAAII
                     ETLLVVTTIDTATATSVAITAHALLFVPPVICGFASFWLVPGIADRLLRFRRAPREVV
                     ATQIVSGGSIPPG"
     gene            complement(1462..2148)
                     /locus_tag="VFV93_02225"
     CDS             complement(1462..2148)
                     /locus_tag="VFV93_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5164187.1"
                     /translation="MTLGMRAYADQVQTAIASIDLAAVERIVDVLEDAFHAGRRVFMM
                     GNGASAALASHMACDLGKGTALDVGQGVADAGLARLKVISLVDNTALLSAISNDVSYQ
                     DVFVEQLKNLLEAGDVVIGISGSGGSPNVLRAMQYARARGATTIGLTGHQPSSRFLCA
                     LSDIVVQVPLTIMEQIEDVHVIMHHMITTALRERIATGRAVKRRVAHGHRLPRLDPLE
                     GALAVQPVSD"
     gene            2455..2736
                     /locus_tag="VFV93_02230"
     CDS             2455..2736
                     /locus_tag="VFV93_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164188.1"
                     /translation="MVADLNPDARGDYESYSAGRAMSGDSLGVRESGSDQLTPSALDP
                     AIFSMLLGRIEQLSRELGRAETQRTLVEQHIARLEQQIERLSRHQETVN"
     gene            2756..4252
                     /locus_tag="VFV93_02235"
     CDS             2756..4252
                     /locus_tag="VFV93_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164189.1"
                     /translation="MSQRRPAAEYELLLDAIERLTFDLGRIEVELADLRHRAARLEAR
                     VVDLNRPVAAQEPLEDTTPEEGVAAESTLDDAAAPETAGAETVVPLLAASEPAVPEAA
                     IDPGIAEPSVVEPAIADPAVAEPAIAELVAEGTSVPEPHALEPELDDQPVFVAAASRH
                     NGVPVFTRIVAEPADERDEPVQSVALVPVLDPVFPVEHEPIWARVPERYRRAAWIAAT
                     LVAVMVVSTWITSADSPSESAPTPHTEAAAGIARTSTPEDAAAFGTATQPATPMSHAG
                     MEMSPTRSASPVATSTGPAAATPTAIPPTPASTEPPSATTPATAAESPTVTAAATNTA
                     PASTVVPTETPAATVAPTVAGQPGQIVPGGVGSTETTIVETFGTADGEQDGRSSYSEG
                     SVLVTYGPGTLAMRVTFLLDTDDLTMSLTDARGLADYYRPPDAQLLSETTNEPGLIRR
                     VYSSTTLGALFAGLDTGGRTADHYVEELRFDPATQRALSIEMALGDRP"
     gene            4547..5662
                     /locus_tag="VFV93_02240"
     CDS             4547..5662
                     /locus_tag="VFV93_02240"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX5164190.1"
                     /translation="MHTYQRNLVEALLARDSGLEYILWADNKAPFDLDKLPAHADLRL
                     LPWSGPLSSVQIDRQLGVAMVRSGADVAHFPANYGFAPSLLPSVITLHDAINIMPLHE
                     IWRGHAKRPKTLAMMTYLHLTTTQALRGRPLVVTVSEHARREILRHSRLDPARVLAIH
                     SAHEPVFRRLDAGAIAEARVRLGLAGRVVLADAIKNPACTLRAWRALPSNIRDSAHLV
                     FFSRREPAPAVRAAAERGEVRLLLRPERDDLVRLYTMADAFVFPSWYEGFGLPVLEAM
                     ACGAPVIASNRGSLPEIAGPAGTIVDAEDHDAIARELTALLTDVDRLAAMRARSLARA
                     AGFSWERTAQRMTDVYTDLALPGRSRLAVRGAPVAAG"
     gene            complement(5728..7947)
                     /locus_tag="VFV93_02245"
     CDS             complement(5728..7947)
                     /locus_tag="VFV93_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164191.1"
                     /translation="MAQSRTPNYRRPAATRLASVVALALLLTGTGFVVWNLDNDTSQP
                     VTAQTSDDTTTPAAPALTSAQAFQRTWQRSDGPVADMTVARTWIWGPASREADQTEAY
                     AESPGGQRAVRYYDKSRMEISDPAGDQQSGWYITNGLLVVELISGRMQTGENRFEQRS
                     PAIENVAGDDGPGNGPTYATLAALLAAPPLPDGSPIVQRIDNSGVVTIDPSLAAYGAT
                     AAFRVTVPGIDHQVASPFWTFMRSSGPIEIDGVTLDGPLFENPFYATGYPLTEPYWTT
                     VKVGGTPHEVLLQCFERRCLTYTPGNDAGWQVESGNVGRHYHDWRYGTDASPTPAPTT
                     IPTPTSTATATSPAPTSTATATSPSSGVAPVVIGPGAVDAWMRSVVRDAEDRVWLVAM
                     NNNAANAGSGPGELRVYRATAPGIPTAFEHVGSALIRATGSSAIPFADAAIDGQNRLH
                     VIWVDYGATGQPLNYRVLDLTSGSWIGPAIMVDDTDLSGFGGFAGQGGVSIAVGFDGQ
                     PRVAYTVAGFQNQIRTRALGPSGWGEASDPLQVSGAWVWHPTLALAGDGTWYLAGYDS
                     TSQTILATHDSGSGWTAAEIVASDVLGPESVDQSPALLVTPAGVPTLTYLDGDSHIRV
                     SQYISTGWTDVPVGGDHHTHAPGLGVYADGTLIIAGHNEGSPPTALNAIQGSDDSWGA
                     WDAVAQIRADGSEVFRWAGAHSTLSADTIDLVFFDEDANDDDVFDDQTLYYLAIPPQ"
     gene            8297..8896
                     /locus_tag="VFV93_02250"
     CDS             8297..8896
                     /locus_tag="VFV93_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX5164192.1"
                     /translation="MATVIQRGIRVARSARVHQSADVAPTAAIGDGAQIWHHAQVREG
                     ATIGDNCIIGKGAYIDADVELGANCKVQNYALVYKGARLGQGVFIGPAAILTNDRYPR
                     AMTPDGELKRADDWECGTIEIEDGASIGAGAVVMTGIRIGAFAMVGAASIVTHDVKPQ
                     ALMIGSPAFHAGWVCKCGRPLVKDLPCFICGDSYEEIVR"
     gene            8893..9999
                     /locus_tag="VFV93_02255"
     CDS             8893..9999
                     /locus_tag="VFV93_02255"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5164193.1"
                     /translation="MIPMARPLVDDAACDAVVAVLRSGMLAQGSVVQQFEERFAELCG
                     VDYAIAASSGTTALHLALLAHDIGPGDEVITTSFSFIASANVALYTGATPVFADIDPA
                     TFQIDPDEVEAKITPRTRAVIAVHLYGTAADLGRLTEICERHNLVLIEDACQAHGATW
                     AGRPVGSFGTGCFSFYPTKNVTSAEGGMVTTNDPEIAERARLLRAHGMPIRYHHDMLG
                     YNFRMTDVHAAIGLAQLDHLRGWTDERVCNAGTLRRLLEPLPVDPQAVYEDASHVYHQ
                     FTIRVHEGRDDVAAYLKSHGVGCEVYYPIPIHQQKVYLDRGYRDFLPHSERAALEVLS
                     LPIHPSLNGEDLCTIARTLADALASVPAMRVAAD"
BASE COUNT         1681 a         3276 c         3496 g         1741 t
ORIGIN      
        1 accggcgtcg atcgccatga cgatgccggc cagatcgtcg gcgccggctg tgacccagat
       61 cggcgtcgtc cgcatctccg gcagggcgcg cagctgacgg cagagttcca gccgctcgtc
      121 gtcgccgcct tcgatctcat cgaggatgac gagctggcag ggttgctcgg tcaggcgtgt
      181 cagcgccgct ggcccgtcag cagcccgcaa cggcgtgtgg ccgcgaatcg agatcgcaag
      241 cgccagccac tcgattgccg gctgaccagt tgagatcacg aggacgttca cgagcgcacc
      301 tgtcgtctga accgaatgat gcgccccctg tcaccgcgtt ccggctcccg gcggccggcg
      361 attggctgcc gccagcctgt caaccgggcg ggattgatcc cccgctgaca atctgcgtgg
      421 caacgacctc gcggggcgcg cggcgaaacc ggagcagccg gtcggcgata cccggcacga
      481 gccagaacga tgcgaacccg cagatgaccg gcgggacgaa cagcagcgca tgggccgtga
      541 tggcgacgct ggtggcggtt gccgtgtcga tcgtggtgac gaccagcagc gtttcgatga
      601 tggcggcgtg gtaaacgcca accgcactcg gcgagattgg caccgcgacg gatgcggccg
      661 aaacgacgac cagcagcatc gcgatcgccg gcgagccttc gacgccaaat gcccgcagcg
      721 tcgcccagag gacgacgccg gtcgtcagcc agttggccgc cgtccagagg atgaccgtcg
      781 ccagccgcgc cggctggccg agaatcgcca gtccatcgac gacatgccgc accatcgagc
      841 gcacccgttc cagcgggccg cgcgccagcc ggccggcgag cggtccgagc cgccgctcaa
      901 gcgctgccct gccgaaccag acgccgcaga taacactgac cccggcgacg gccaggacaa
      961 ccgtgatccg cgtcgattcg gcggcccact tcggcaatga gacgaacggc agcagcatga
     1021 cgatgatgac cagcagggca acgacatcca gcacccgctc gacaacgacg gtcgtcagcg
     1081 cccaggcctt gctgcgacgc tccatctcac cggccaggta ggcccgcacg acatcgccga
     1141 gccgcagcgg gagcaacgag gtcagcgcgg cgccgacact gaggatcccg aacgcacgcg
     1201 tgaaggacat ctgcgcgggc cagcagagca gccgccagcg ctgggcgcga atcgcgaccg
     1261 acaggaacga ggcggcgatc gccagcagca gccagtcgta gcgcgccgtt cggaaggcct
     1321 cggcgacatc gtcgaggttg acccgcatca ggacgatcgc gatcgcgccg gcgctgatga
     1381 gcagccccag tgcgagtcgg gtccatggca gtccctggaa catgggtgca accctttcgc
     1441 gagcgcgctc gctgactgtc gtcagtcgga gaccggttgg accgccagcg caccctcaag
     1501 cggatcgagc cgcggcagcc ggtgcccgtg ggcgacgcgc cgcttgacgg cccggccggt
     1561 cgcgatccgc tcgcggaggg cggtggtgat catgtggtgc atgatgacgt ggacatcctc
     1621 gatctgctcc atgatcgtca gtggcacttg gacgacgatg tcgctcagcg cgcagaggaa
     1681 ccggctgctg ggctggtgac cggtcagacc gatcgttgtc gctccccggg cgcgggcgta
     1741 ctgcatcgcg cgcagtacgt tgggcgaccc gccgctgccg ctgatcccga tcacgacgtc
     1801 accggcctca agcaggttct tgagttgctc aacgaagaca tcctggtagc tgacatcgtt
     1861 gctgatggcc gacagcaggg ccgtgttgtc cacgagactg atgactttca ggcgggccag
     1921 gcccgcgtcg gcgacgccct ggccgacatc gagggccgtg cccttgccga ggtcgcaggc
     1981 catgtgcgac gcgagcgccg cgctggctcc gttgcccatc atgaagacgc gccgtcctgc
     2041 atggaaagcg tcttcgagga catcgacaat ccgttcgacg gcggcgaggt cgatactggc
     2101 aatagcagtc tggacctggt cggcgtatgc gcgcatgccg agcgtcattc gattcgtcct
     2161 cgctttccgt catccgcatg ggcgaccgcc gatatatccc gacggcgcgg cgcgttgaca
     2221 acctgttgca tccctcgcgg ctgcaggtag cgttcagtga atcgtttcga caccttcccg
     2281 tttccatcga tcattcgccg gtgaggtgag agaattgaca ggtagcgtgt tctaccggac
     2341 agagacatcg tggataatgg cgggtacgct acgtcgtacc ggctgccgcg gaggtagtcc
     2401 ggaccgtcgt tccaatcggt aacgcgggtc gcgggaggaa aaggagcctg attgatggtg
     2461 gccgatctca atcctgatgc gcgtggtgac tacgagtcgt atagtgccgg ccgggcgatg
     2521 tcgggtgata gcctgggggt tcgcgagagt ggcagtgatc agcttacacc gtcggcgctc
     2581 gacccggcga tcttctcaat gttgctgggc aggatcgagc agctatcgcg agagcttggc
     2641 cgggccgaga cgcagcgcac gctggtcgaa cagcatattg cccggctgga gcagcagatc
     2701 gagcgactct cgcggcacca ggaaaccgtg aactgacagg atcgggaaat ccgcgatgag
     2761 ccagcgacgg cctgccgctg aatacgaatt gctgctcgat gcgatcgagc ggctgacgtt
     2821 tgacctgggc cggatcgagg ttgagctcgc cgacctgcgc caccgggcag cccggctcga
     2881 ggcgcgcgtc gtcgacctca accggccggt cgcggcccag gagcctctgg aagacaccac
     2941 tccggaagag ggcgtcgccg cggaatccac gctggatgat gccgctgccc cggaaactgc
     3001 cggcgcggag accgttgtcc cgctgcttgc tgcatccgag ccggcggtgc ctgaggctgc
     3061 cattgatccg ggcatcgcgg aacccagcgt cgtcgaacct gccatcgctg atccggctgt
     3121 tgctgagccc gccatcgcgg aactggttgc cgagggaacc agcgtcccgg aaccccacgc
     3181 tttggaacct gaactcgacg atcagccggt gtttgtggca gcagcttcgc gccataacgg
     3241 cgtgccggtc ttcaccagga tcgtcgcgga accagcggat gagcgcgacg agccggtgca
     3301 gtcggtcgcg ctggtgccgg ttctcgatcc ggtgttcccc gtcgagcacg agccgatctg
     3361 ggcgcgcgtg ccggagcgct atcggcgggc ggcctggatc gcggctacgc tggtcgcggt
     3421 gatggtcgtc tcgacctgga tcacatcggc cgactcacca tcggaatccg ctccgacacc
     3481 gcataccgag gctgcagcgg gcatcgcacg aacgtcaaca ccggaagacg ccgcagcgtt
     3541 tggcacggca acgcagccgg cgacgccgat gtcgcacgcc ggcatggaga tgtcgccgac
     3601 cagatccgcc tcgccggtgg cgacatcgac cggccctgct gccgcaacgc caacggcgat
     3661 cccgcccacg ccggccagca ccgaaccgcc gtctgccacg acaccggcga cagccgccga
     3721 gagtccgaca gtgacggcag cggcgacgaa cacggccccg gccagcacgg tggtcccgac
     3781 cgagacgccg gccgcgacgg tggcgccgac ggttgccggc cagccgggcc agatcgtgcc
     3841 gggcggggtt ggcagcaccg agacgacgat tgtggagacg ttcgggacag ccgatggcga
     3901 gcaggacggc cggtcgtcct acagtgaggg cagtgtcctc gtcacctacg ggccgggcac
     3961 gctggcgatg cgggtgacgt tcctgcttga tacggatgat ctgacgatgt cgctgacgga
     4021 cgctcgcggg ctggccgact actaccgccc accggacgcg cagttgctga gcgagacgac
     4081 caacgagccg gggctgattc ggcgggtcta ctccagcacg accctcggcg cgctcttcgc
     4141 cggcctcgac accggcggcc gaacggccga ccactacgtc gaggagctgc gcttcgatcc
     4201 agcgacccag cgcgcgctct cgattgaaat ggcgctcggc gaccgcccct gaccgtagcg
     4261 gctgacgagc gggacgcgaa gacctgagct tcgtcgttgc taagccattg gcttcgcgac
     4321 agtgtgacca ccctctgtac aacctgtcag cgccagcctg cggagttgcc gtctgtcgtc
     4381 cctcgacgat ccggcagact cgctggtgaa ccaggtgagc gtgccacgcg ttcgccgccg
     4441 gcgggtgcag tcgatgccag agcgcgagat gaggtctcca atgtcgttga gatgtcctcg
     4501 aattgcgata gatgttcgct acctttcaca cgggctggtt ggcggggtgc atacctacca
     4561 gcgcaacctg gtcgaggcgt tgctagcgcg tgacagtggc ctggagtaca tcctctgggc
     4621 cgacaacaag gcgcctttcg atctggacaa gcttccggcg catgccgatc tgcggctgct
     4681 gccgtggtcg ggcccgctca gcagtgttca gatcgaccgg caacttgggg tcgcgatggt
     4741 gcggagtggg gccgatgtgg cccacttccc cgccaactac ggatttgcgc cgtcgttact
     4801 gccgtcggtc atcaccttgc acgatgcgat caacatcatg ccgctgcatg agatctggcg
     4861 cgggcacgcc aagcgcccga agacgttggc gatgatgacg tatctgcatc tgacgacgac
     4921 ccaggcgctg cgcgggcgac cgctggtcgt gacagtcagc gagcatgcgc ggcgtgagat
     4981 tctgcggcat tccaggctcg atccagcgcg ggtgttggcc atccattcgg cgcacgagcc
     5041 agtcttcagg cggctggatg ccggcgcgat cgccgaggcg cgagtgcggc ttggtctggc
     5101 cggtcgggtg gtccttgccg acgcgatcaa gaatccagcc tgtaccctgc gggcgtggcg
     5161 cgcgttgccg tcgaacatca gagactcggc gcatctggtc ttcttctccc gtcgcgagcc
     5221 cgctccggct gtgcgcgcgg ctgccgagcg tggcgaggtg cgactgctgc tgcggccaga
     5281 gcgagatgat ctggtgcggc tctataccat ggccgatgcg ttcgtcttcc cgtcctggta
     5341 cgaaggtttc gggctaccag tgctggaggc gatggcgtgt ggagcgccgg tgatcgcctc
     5401 gaaccgtggc tcgttgccgg aaatcgccgg accagccgga acgatcgttg atgccgaaga
     5461 tcacgatgct attgcgaggg agttgacggc gctcttgacc gacgtagacc ggctggcggc
     5521 gatgcgcgca cgctcgctgg cgcgggcagc cgggttctcg tgggagcgga cggcgcaacg
     5581 gatgactgat gtctacacgg atctggcgct gccgggccga tcgcgtctgg ccgtgcgggg
     5641 tgctccggtc gcggccggct gagtcgtacg ggtaatgctg gtgacttcaa cgatcactca
     5701 cgtagacgat tctcgctgac gtcgcggtca ctgcggggga atggcgaggt agtagagggt
     5761 ctggtcatcg aagacatcgt cgtcgttggc gtcttcgtcg aagaagacga gatcgatcgt
     5821 gtcggccgac agggtcgagt gggcgccggc ccagcggaag acctcggagc catcggcccg
     5881 gatctgggcg acggcgtccc aggcgcccca gctgtcatcg ctgccctgga tggcgttgag
     5941 ggcggtcggc ggtgagcctt cgttgtggcc ggcgatgatg agcgtgccat cggcgtagac
     6001 gccgagcccc ggcgcgtggg tgtggtggtc gccgccgact ggcacatcgg tccagccggt
     6061 gctgatgtac tggctgacac ggatgtgtga gtcaccatcg aggtaggtca gcgtcgggac
     6121 gccggccggg gtgacgagca gcgccgggct ctggtcgacg ctctccggcc cgagtacatc
     6181 gctggcgacg atttcggcgg ctgtccagcc gctgccgctg tcgtgggtgg cgaggatcgt
     6241 ctgactcgtc gagtcgtagc cggccaggta ccaggtgccg tctcctgcca gcgcgagtgt
     6301 agggtgccag acccaggcgc cgctgacctg gagcgggtcg ctggcctcgc cccagcccga
     6361 gggaccgagc gccctggtcc ggatctggtt ctggaagccg gcgacggtgt aggcgacgcg
     6421 gggctggccg tcgaatccga ccgcgatgct gacgcctcct tggccggcga atccgccaaa
     6481 cccgctcaga tcggtgtcgt cgaccatgat ggccggcccg atccacgaac cgctggtgag
     6541 atccagcacc cggtagttga gaggctgccc ggtcgcgccg taatcgaccc agatgacgtg
     6601 cagccgattc tgtccgtcga tggcggcgtc agcgaaaggg attgcgctgg aaccagtggc
     6661 ccggataagt gccgatccaa cgtgctcgaa agcggttgga atgcctggtg cggtcgcgcg
     6721 atagacccgt agctcgccgg ggccgcttcc ggcgtttgcc gcgttgttgt tcattgccac
     6781 caaccagacg cgatcttcgg catcgcggac gaccgagcgc atccaggcgt cgaccgcacc
     6841 ggggccgatg acgaccggcg cgacgccact ggatggcgat gtcgcggtcg ctgttgaggt
     6901 tggagcgggc gatgttgcgg ttgccgtcga ggtcggagtg ggtatcgtgg ttggtgccgg
     6961 cgtgggagac gcatcggtgc cgtagcgcca gtcgtggtaa tggcggccga cattgccgga
     7021 ttccacctgc cagccggcgt cattacctgg tgtataggtc agacagcggc gttcgaagca
     7081 ttgcagcagg acctcgtgcg gggtgccgcc gaccttgacg gttgtccagt acggctcggt
     7141 aagtgggtag ccggtggcgt agaacgggtt ttcgaagagc gggccatcga gtgtcacgcc
     7201 gtcgatctcg attggtccgg acgaccgcat gaacgtccag aatggcgatg cgacctggtg
     7261 gtcgatgcct ggcactgtta cccggaaagc ggccgtcgcg ccatatgctg ccagggaagg
     7321 gtctatcgtc accacgcctg agttgtcgat gcgctggacg atcggactgc cgtcgggcag
     7381 cggcggggcg gccagcaggg cagccagcgt cgcgtaggtc gggccgttgc cggggccgtc
     7441 gtcgccggcc acgttctcga tggccgggct gcgctgctcg aagcggttct cgccggtctg
     7501 catccggccg ctgatcagtt cgacgaccaa cagcccgttc gtgatgtacc agcccgattg
     7561 ctggtcgccg gcggggtcgc tgatctccat gcgagacttg tcataatagc ggaccgcgcg
     7621 ctggccgccg ggagattcgg cgtatgcctc agtctggtca gcctcacgtg acgcggggcc
     7681 ccagatccag gttctggcga ccgtcatgtc ggcgaccggg ccgtcactcc gttgccaggt
     7741 gcgttgaaac gcttgcgcgc tggtcaatgc gggtgctgct ggcgtcgtag tgtcgtcact
     7801 ggtctgggcc gtgacgggct gggacgtgtc gttgtcgagg ttccagacga cgaaaccggt
     7861 gccggtcagg agcagcgcca gcgccacgac tgatgcgagc cgcgttgctg ccggccgacg
     7921 atagttcggc gtccttgatt gcgccacgct cacacgctac ttcgataaat caccaaccgg
     7981 gtcttgccgg ttggggtgct atcacgatct gtcagtgtca tggtcatctt gctgacagcc
     8041 tgcgggctgc gcctgccagc ctttgggatg cccgacggac cggttcctga caggttggtc
     8101 agcggggttg ctatctgaca ctcgcacacc gagctgaacg gctgcaaacg ttcgatgtga
     8161 ccgatttgtc actgccgaga ggaccgattg cgcatcagga accggtcgcg gccactaggc
     8221 tggtgacatc cgaatggcgc gttgcgccgt gatagcaccg acaccgatgc aggtggcaga
     8281 accgggagcg atgtggatgg cgactgtcat tcaacgtgga attcgggtgg cgcgcagcgc
     8341 gcgtgtccat cagtcggccg atgtggcccc gacagccgcc attggcgatg gcgcacagat
     8401 ctggcaccac gcccaggttc gcgaaggggc gaccatcggc gacaactgca ttatcggtaa
     8461 aggcgcgtat atcgacgccg atgtcgaact gggcgcgaat tgcaaggtcc agaactatgc
     8521 actggtctac aagggcgcgc ggctcgggca gggtgtgttc atcggtccgg ccgcgattct
     8581 gaccaatgat cgctacccga gggcaatgac acccgacggc gagctcaagc gagccgatga
     8641 ctgggaatgc ggcacgatcg agatcgagga cggcgcgtcc atcggcgccg gcgcggtcgt
     8701 gatgacgggt atccgaatcg gtgccttcgc gatggttggc gcggcctcga tcgtgaccca
     8761 cgatgtcaag ccacaggcgt tgatgatcgg cagccctgcc tttcacgccg gctgggtctg
     8821 caagtgcggc cggccgttgg tgaaggatct gccgtgcttc atctgcggcg atagctatga
     8881 ggagattgtc cgatgattcc gatggcacgt ccgctggttg atgacgcggc ctgtgatgcc
     8941 gtcgttgccg tcctgcgttc gggaatgctg gctcagggat cggtcgttca gcagttcgag
     9001 gagcgcttcg ctgaactgtg tggcgtcgac tacgccatcg cggcctcgtc ggggacgacg
     9061 gcgctgcacc tggcgctgct ggcgcacgac atcggccctg gcgacgaggt catcacgacc
     9121 tcattctcgt tcatcgccag cgcgaatgtc gcgctctaca ccggcgcgac gccggtcttc
     9181 gccgacatcg acccagccac attccagatt gaccccgacg aggttgaggc caagatcacg
     9241 ccccggacgc gggccgtcat cgccgtccat ctctatggca cggcggccga ccttggccgg
     9301 ctgacggaga tctgcgagcg acacaacctg gtcctgatcg aggacgcctg ccaggcgcac
     9361 ggcgcgacct gggccggccg gccggtcggc tcgttcggca ccggctgctt ctcgttctac
     9421 ccgaccaaga acgtcacctc ggccgaaggc gggatggtga cgaccaacga cccggagatc
     9481 gccgaacggg ccagactgct gcgtgcccac gggatgccga tccgctacca tcacgacatg
     9541 ctgggctaca acttccggat gacggacgtc catgccgcga tcgggctggc gcagctcgac
     9601 cacctgcgcg gctggaccga cgagcgtgtc tgcaacgccg gcacactccg gcggctgctg
     9661 gagccgctgc ccgttgaccc gcaggccgtc tacgaggacg ccagccacgt ctatcaccag
     9721 ttcacgatcc gcgtccacga gggccgcgac gatgtcgccg cctacctgaa gtcacacggt
     9781 gtcggctgcg aggtctacta cccgatcccg atccatcagc agaaggtcta cctcgatcgt
     9841 ggctaccggg acttcctgcc gcacagcgag cgagctgccc ttgaggtgct ctcgttgccg
     9901 atccatccgt cactcaacgg cgaggacctc tgcacgatcg ccagaacgct ggctgatgcg
     9961 cttgcgagcg tcccagcgat gcgggtcgcc gccgactgac aaccacttct gattactccc
    10021 acacttcacc ccccgagcga gccggcctca ccacccggct cgctcgctgt ctggcgacct
    10081 gacgccgtcg tcggcggttc cccaaccccg tgtaggggcc gatcctgtat cggcccgcgc
    10141 cgtaaggcgc ccacgtggag cgcgggcggt tgatccacgg gcgcaggcgt tggg
//
LOCUS       DASUGJ010000100         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1402807, whole genome shotgun sequence.
ACCESSION   DASUGJ010000100 DASUGJ010000000
VERSION     DASUGJ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402807"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VFV93_02260"
     CDS             complement(<1..897)
                     /locus_tag="VFV93_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164194.1"
                     /translation="MSERLIAPYGSWESPITSDLIASGSVRLGHVTLDGDSLYWLESR
                     PSEGGRSVLVRRGQDGTVEDVTQPGSNVRTRVHEYGGGSYLISGDAIFYSDFANQLLH
                     RKDKGDDAFPISPEPNIPAGLRYADMRLTPDGRTLVAVQEEHDGTNEAVNSIVSMPAV
                     GTSEPVTIALGRDFYAFPRVSPDGRQIAWIEWDHPNMPWDGTELWVADLASDGNASHP
                     RRIAGGPEESIFQPDWSPEERLHFISDRTNWWNLYRVEADGTITPLAPIEAEIGQPQW
                     VFDLASYVFLSGGRIACIVNENG"
     gene            1283..1882
                     /locus_tag="VFV93_02265"
     CDS             1283..1882
                     /locus_tag="VFV93_02265"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5164195.1"
                     /translation="MIDEQPVLNIIGERVALGPLRRDLIPLYARWRNDFYVQRTFGNL
                     PIPVSIEQRTAQFEKMATSTDAYWFTIYEKETLRPIGTTDLFDVDFRWRVGNFGIMIG
                     EAEARGQGYGTEATRLMLDYAFTALGLNNVMLTVAEYNLAGRRAYEKAGFKEFGTRRQ
                     ADIMAGVVYDEIYMDCIASEFDSPVLGRIFAPDERRDAE"
     gene            1888..>2391
                     /locus_tag="VFV93_02270"
     CDS             1888..>2391
                     /locus_tag="VFV93_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164196.1"
                     /translation="MNSVSASRVPRPASRTPSPLLLVISLIGAASFLYPFFLPALAGV
                     GARTARGGIEVPLVFTALGLACLLVILLEVQGRAGIGDQGASKLVALLGMLVAVDATL
                     RLVPSLLGASPIFLLIVLVGFAYGAEIGFLMGALTLFVSAIITGGLGPWLPYQMLGAG
                     WVGMTAGW"
BASE COUNT          379 a          792 c          794 g          426 t
ORIGIN      
        1 gccgttctcg ttgacgatgc aggcgatccg cccgccggag aggaagacgt acgaggccag
       61 atcgaagacc cactgcggct ggccgatctc cgcctcgatc ggcgccaacg gcgtgatcgt
      121 gccgtcggcc tcgacccggt agaggttcca ccagttggtc cggtcggaga tgaagtgcag
      181 ccgctcctcc ggcgaccagt cgggctggaa gatcgactcc tccggcccac cggcgatccg
      241 gcgcggatgc gaggcgttcc catccgacgc gaggtccgcc acccacagct cggtgccgtc
      301 ccacggcatg ttggggtggt cccactcgat ccaggcgatc tgccggccat cgggactgac
      361 gcgcgggaag gcgtagaagt cgcggccgag cgcgatcgtc accggctcgg acgttccgac
      421 ggccggcatc gacacgatcg agttgacggc ctcgttcgtc ccgtcgtgct cttcctggac
      481 ggccaccaac gtccggccat ccggcgtgag gcgcatgtcg gcgtagcgca ggccggcggg
      541 aatattcggc tccggcgaga tcgggaaggc gtcgtcgcct ttgtccttgc ggtgcagcaa
      601 ctggttcgcg aaatcgctgt agaagatcgc gtcgccgctg atcaggtacg agccaccacc
      661 gtactcgtgg acccgcgtcc ggacgttgga gcccggctgg gtcacgtcct cgaccgtgcc
      721 atcctgccca cgccggacga ggaccgagcg cccgccctcg ctgggccgcg attcgagcca
      781 gtagaggctg tcgccatcca gcgtgacgtg ccccagcctg accgagccgc tcgcgatcag
      841 atcggacgtg atcggcgact cccacgagcc gtacggggcg atcagtcgct cagacatggc
      901 aacactcctc attcccaatt ccctgaccta ccggtcgcgc tacttgacgg tatcgaggtc
      961 cgcgacgaag atcaatggca cacaaaccgt aaccggaagc tgacgtcctt gtgattgtga
     1021 catcccgtgg acgacgcaac agtcaccgat ctcgccacca gtgtaccggc agccagcgtc
     1081 ccatttgaac tcagaacatc cagccgtcaa ccggcgctcg ctgatcgcgc cggcacccgc
     1141 aacgtgcgcg tcgcccccgg tcaagctacc tggggcagcg cgtcatagcg tgccattcgg
     1201 agcctgcgtc accccactcg gattccgccc gttctggtat cgtctcggtt cgaacgagaa
     1261 acggacgagt tcggggagtg gcatgatcga cgagcaacct gttctgaata tcatcggcga
     1321 gcgggtggcg ttggggccgt tgcggcggga tctgatcccg ctgtatgccc gctggcgcaa
     1381 cgacttctac gtccagcgca cgttcggcaa cctgccaatt ccggtctcga tcgagcagcg
     1441 gacggctcag ttcgagaaga tggcgacatc gactgacgcc tactggttca ccatctatga
     1501 aaaggagacg ctgcgcccga tcgggacgac cgatctgttc gacgtcgatt tccgctggcg
     1561 cgtcgggaac ttcgggatca tgatcggtga ggccgaagcg cgcggtcagg gctacgggac
     1621 cgaggccacc cggctgatgc tggactacgc cttcaccgcg ctcggactca acaacgtcat
     1681 gctgaccgtc gccgagtaca acctggctgg ccggcgtgcc tacgagaagg ccggctttaa
     1741 ggagttcggc acgcgccgcc aggccgacat catggccggc gtcgtctacg acgagatcta
     1801 catggactgc atcgccagcg agttcgacag cccggtcctg gggcggatct tcgcgccgga
     1861 cgaacgtcgg gacgcggagt aaacccgatg aacagcgtat ccgcgtcccg cgtcccgcgt
     1921 cccgcgtccc gaacaccttc ccccctcctc ctcgtcatca gcctgatcgg cgccgcgtcg
     1981 ttcctgtatc cgttctttct gccggcgctg gccggggttg gggcgcggac ggcgcggggt
     2041 gggatcgagg tgccgttggt cttcacggcg ctggggctgg cctgcctgct ggtgatcctg
     2101 ctggaggtgc aggggcgggc cgggatcggt gaccaggggg cgtcgaagct ggtcgcgctg
     2161 ctggggatgc tggtcgccgt cgatgcgaca ctgcggctgg tgccgtcact actgggcgcc
     2221 tcgccgatct tcctgctgat cgtgctggtt ggcttcgcct atggcgccga gatcggcttc
     2281 ctgatgggcg cgctgacgtt gttcgtctcg gccatcatca ccggcgggct ggggccgtgg
     2341 ctgccgtacc agatgctggg ggccggctgg gtcgggatga cggccggctg g
//
LOCUS       DASUGJ010000101         5025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1124904, whole genome shotgun sequence.
ACCESSION   DASUGJ010000101 DASUGJ010000000
VERSION     DASUGJ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124904"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1211
                     /locus_tag="VFV93_02275"
     CDS             33..1211
                     /locus_tag="VFV93_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5164197.1"
                     /translation="MSDHVDSSFRQRRLLSRRRFMQLAAITAGGLLVPACMGDDDPEP
                     SPTATTAAPTEEVIVPAVSGYDNPTVWEGRTLTVTSWGGEYEDAQRDAIFEPFERLTG
                     AKIEVAATDIAQLRKMVEDEDTDWDVSDVLTEDVLPFANLGVIETIDYNVVDPDGLFS
                     EARLDHGVASSFYSTVLTYRTDRWPDLPPPSSWRDFWDAERYPGTRGLHRQPQGTLEF
                     ALLADGVALDGLYPLDVTRAFAALDRILPEMTLWWEQGAQPAQMVTSGDLDMVSAWHS
                     RIYRLESEAEKVGISWNGGAISGDSWVISKGAPNLEVAMDFINFATRPEVCAAFSKLV
                     PFGPVNSKTFDLLPPDIVERLPTAPQHKDVQFTINFEWWFQHREAVEAEFEDWMAEHP
                     "
     gene            1488..2690
                     /locus_tag="VFV93_02280"
     CDS             1488..2690
                     /locus_tag="VFV93_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5164198.1"
                     /translation="MVTTTTLRDEIDEWLPELIETRRDFHRNPELGLEEVRTSGIVAE
                     RLRAIGFEDVRTGLAITGVKAVLRGGKPGRTLLLRADMDALPIEEQNDVEYRSTVKGT
                     MHACGHDGHTAILLGAARALYARRDEIPGTIVFCFQPAEEGRGGARRMIEDGVLENPQ
                     VDAAIGLHLISEFQVGTAVAVDGPTLSGADRFNVTIQGSGGHGAMPHKTVDALLIAAE
                     VVTTLQNLVAREVNPQQSAVVSVGTLHAGTTAANIIADSATLSGTVRWFDADVGDLLA
                     CRVPEVIQGIATTLRGSAEVEYRRGGRPTVNDPAVAALVREALAEVVGTDNVHDGPRV
                     MGSEDFSEFTHRLPSTFFFVGCRNESTGIVAEHHHPRFDIDEGSLPLGVEMMTRAALR
                     WLETHAAD"
     gene            2711..3505
                     /locus_tag="VFV93_02285"
     CDS             2711..3505
                     /locus_tag="VFV93_02285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5164199.1"
                     /translation="MSEKPTIDQDLRDRVQRHFGATAQDYVTSARHASGGGLDQLVEL
                     AEPTPESDALDIATGGGHTALALAPHVRHVVASDLTPNMLAAAKAFLTEKGITNASFE
                     IAGAENLPFADDAFDIVTCRVAPHHFSDVQAFCREVARVLRPGGRFILIDSYAPDDDA
                     LDEYINHVEWARDNSHARAWRVPEWEQFIAVTGLTIDHVEPFVRRFEFEEWTARTRMS
                     PEAKAELEHYMLSAPESVRDFFHVTKTPDGHVDGFDDNKVIIRARA"
     gene            complement(3538..3942)
                     /locus_tag="VFV93_02290"
     CDS             complement(3538..3942)
                     /locus_tag="VFV93_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5164200.1"
                     /translation="MVIDQTTQRALEQFGHEWVDVELSGDAGQLANFLTDDFICVGPF
                     GFVINKEQYLHGRRNGDLKHTAFDWSEVQLRLHDDVAIAVGKQTQTSAFQNRDASGTF
                     RATQVLVQNGNGWKIASLHLCAIAPPPNMGGA"
     gene            complement(3957..4841)
                     /locus_tag="VFV93_02295"
     CDS             complement(3957..4841)
                     /locus_tag="VFV93_02295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5164201.1"
                     /translation="MKLGLHINDFGWPIETQQLATTIADIGQAAEEAGFARIAVMDHV
                     WQHPYAGGGPEGPVLEAYSTLAFLAARTNRTDLIALATPVSYRNPAMLVKTVTTLDVL
                     SGGRAWLGIGVGDYEDEATGLGIPYPPTAERYELLDETLQICLRMWSGEHGDDEPFGG
                     KHTRLGRALNVPQSLRRPHPPILIAGSGERKTLPLVARYADACNIRPGPDLSGRLDVL
                     RRLCDVEGRDYDAIEKTCPFYFDVGENGSNVGALIEQLRQFAKLGIQTVYGRVVNDHQ
                     ITPIEIMGREVIPAIADD"
BASE COUNT          902 a         1695 c         1548 g          880 t
ORIGIN      
        1 cccggcgagg acgcggacga ggtttcaacg acgtgagcga tcacgtcgac agttcgttcc
       61 ggcagcggcg attgctcagc cgtcgccggt tcatgcaact ggccgcgatc actgccggcg
      121 gactgctggt gcccgcctgc atgggcgatg acgatccgga accatcacca acggcaacga
      181 cggccgcgcc gactgaggaa gtcatcgtcc cggcggtgtc tggctacgac aacccgaccg
      241 tctgggaggg tcggacacta actgtcacca gttggggtgg cgagtacgag gacgcccagc
      301 gtgacgcgat cttcgagccg ttcgagcgct tgaccggggc gaagatcgag gtcgccgcga
      361 ccgacatcgc ccagctgcgc aagatggtcg aggacgaaga caccgactgg gatgtctcgg
      421 atgtccttac cgaagacgtt ctgccattcg ctaacctcgg cgtcatcgag acgatcgact
      481 acaacgtcgt cgatcctgac ggactcttct cggaagctcg actcgatcac ggcgtcgcct
      541 ccagcttcta ctcaacggtt ctgacctatc gcaccgatcg ctggcccgac ctgccaccgc
      601 cgtcctcgtg gcgagacttc tgggacgctg aacgctaccc cggcacgcgc gggctccacc
      661 gccagccgca aggcacgctg gagtttgcgc tgctggccga cggcgtcgcg ctggacggac
      721 tctacccgct ggacgtcaca cgtgccttcg ccgccctcga tcgcatcctg ccggagatga
      781 cgctctggtg ggagcagggc gcgcagccgg cccagatggt caccagtggc gatctcgaca
      841 tggtcagcgc ctggcacagc cgcatctatc gcttggagag cgaagcggag aaggtgggca
      901 tcagttggaa cggcggcgcg atctctggcg actcgtgggt catctccaag ggcgcgccaa
      961 acctcgaggt cgcgatggat ttcatcaact tcgcgacccg cccggaggtc tgcgcggcct
     1021 tctcgaagct ggtgccgttc ggcccggtca actcaaagac gttcgacctt ctcccacccg
     1081 acatcgtcga acgcctccca accgccccac agcacaagga cgtccagttc acgatcaact
     1141 tcgaatggtg gttccagcat cgcgaagccg tcgaagccga gtttgaagac tggatggcag
     1201 agcacccgta gcagcgttcc cacatgttcg gccgaggctg cggcgctggc ctgggccgct
     1261 ccgcgaggtt gagtctgacc aagcttcgag tctgaagaga atctctcaac atctgcgccc
     1321 gtggatcatc ccaattgagc gtaaccagac gtcacgcgac gcttaccgac gttgcgattg
     1381 tagatgggct cccaacctgt gaaactgccc acacgacagc cccggctcac ctcaattgcg
     1441 ctgctatact gcgcccgagg acgggaaaag gggaggaacc taccgcgatg gtcacgacca
     1501 caacgctgcg agacgagatc gacgaatggt tacctgagct gatcgagacg cggcgtgact
     1561 tccaccgcaa ccccgagttg gggctggagg aagtgcgcac cagcgggatc gttgccgaac
     1621 gcttgcgggc gatcggcttc gaggacgttc ggaccggcct ggccatcacc ggcgtcaaag
     1681 ccgtcctgcg tggaggaaag cccggcagga cgctgctgct gcgggctgac atggacgctc
     1741 tgccgatcga ggagcagaac gacgtcgagt accgctcgac cgtcaagggg acgatgcacg
     1801 cctgtgggca tgacggccat accgccatcc tgctcggcgc ggcccgtgcg ttgtacgctc
     1861 ggcgtgacga gatccccggc acgatcgtct tctgcttcca gccggccgag gagggacgcg
     1921 gcggcgcgcg acggatgatt gaagatggcg tcctcgaaaa ccctcaagtc gacgccgcga
     1981 ttggcctgca cctgatcagc gagtttcagg tcggcacggc tgtcgcagtc gatggtccga
     2041 ccctttcggg tgccgaccgc ttcaacgtca caatccaggg tagtggcggc catggcgcga
     2101 tgccacacaa gacggttgat gcgctgctga tcgcggccga ggtcgtcacg acgctccaga
     2161 acctcgttgc ccgcgaggtc aacccacagc agtcagccgt cgtctcggtc ggcacattgc
     2221 acgccggcac gaccgccgcc aacatcatcg ccgactcagc caccctgagc ggcaccgtcc
     2281 gctggttcga cgccgatgtc ggcgacctgc tggcgtgtcg cgttcccgaa gtcatccagg
     2341 gcatcgccac taccctgcgc ggcagcgccg aggtcgagta ccgccggggc ggtcgcccta
     2401 ccgtcaacga cccggcagtc gcggcgctgg tgcgagaggc attggctgag gtcgtcggca
     2461 ctgacaacgt tcacgacggc ccccgcgtga tgggctcgga ggatttctcc gagttcaccc
     2521 accggctgcc atcgaccttc ttcttcgtcg gctgccgcaa cgagagcacc ggcatcgttg
     2581 ctgaacatca ccacccacgc ttcgacatcg acgagggctc gctaccgctc ggcgtcgaga
     2641 tgatgacccg tgctgcgctg cgctggctcg aaacccacgc cgcagattga tcgtgtcaag
     2701 gagccgaaca atgtccgaga agccaacgat cgaccaggac ctgcgcgacc gagtccagcg
     2761 ccacttcggt gcaacggcgc aggattacgt cacctcagcc cggcatgcct ccggcggcgg
     2821 cctcgaccag ctcgttgaac tggccgagcc aacgcccgag tccgacgcgc tcgacatcgc
     2881 caccggcggc ggccatactg cgctcgcgct cgcgccccat gtccgtcatg tcgtcgccag
     2941 cgacctgacg ccgaacatgc tggcggccgc gaaagcgttc ctgaccgaga agggcatcac
     3001 caacgccagc ttcgagatcg ccggcgccga gaatctgccc tttgctgacg acgcattcga
     3061 catcgtcacc tgccgcgtcg ccccgcacca cttcagcgat gtccaggcct tctgccgcga
     3121 ggttgctcgc gtcctccggc ccggcggacg tttcatcctg atcgactcct acgccccgga
     3181 cgacgatgcc ctcgacgagt acatcaacca cgtcgaatgg gcccgcgaca actcgcacgc
     3241 ccgtgcctgg cgcgtccccg agtgggagca gttcatcgcc gtcaccggcc tgacgatcga
     3301 ccacgtcgaa cccttcgtcc gccgcttcga gttcgaagag tggactgccc gcacgcggat
     3361 gtcgccggag gccaaggcgg aactggaaca ctacatgcta agcgcgccgg agtctgtccg
     3421 cgacttcttc cacgtcacaa agacgccgga tggccacgtc gacggcttcg acgacaacaa
     3481 ggtcatcatc cgcgcccgtg cgtagcggcg ggcatcaata gtaatcgtca atccgctcta
     3541 cgcaccgccc atattcggcg gcggcgcgat cgcgcaaaga tgcaggctgg cgatcttcca
     3601 gccgttcccg ttttggacca gcacctgcgt cgcccggaat gtaccggacg cgtcacggtt
     3661 ctggaaggcg ctcgtctgcg tctgcttccc gactgcgatt gcgacgtcat cgtggagacg
     3721 caactgcacc tcgctccagt cgaaggcggt gtgcttcagg tcgccgttgc ggcggccgtg
     3781 caggtactgc tccttgttga tgacgaatcc gaacggcccg acacagatga agtcgtccgt
     3841 caggaagttc gccagttgcc cggcatcacc ggagagctcg acgtcaaccc actcgtggcc
     3901 gaactgttcc agtgcgcgct gtgtcgtctg gtcgattacc atcctgtgtt gtcctctcag
     3961 tcatcggcga ttgctgggat gacctcgcgt cccatgatct cgatcggagt gatctggtgg
     4021 tcgttgacga cgcggccgta gacggtctga atgcccagtt tggcgaactg gcgcagttgc
     4081 tcgatcaacg cgccgacatt cgagccgttc tcgccgacat cgaagtagaa cggacacgtc
     4141 ttctcgatcg cgtcgtagtc gcggccctca acgtcgcaga gacgccgcag aacatccagc
     4201 cggccggaca agtccggacc ggggcggatg ttgcaggcgt cggcgtagcg cgcgaccagt
     4261 ggcagcgtct tccgctcgcc gctaccggcg atcaagatcg gaggatgtgg tcgtcggagg
     4321 ctttgtggca cgttcaacgc ccggcccagc cgcgtgtgct tcccgccgaa cggctcgtcg
     4381 tcgccgtgct cgccactcca catccgcagg cagatctgca gcgtctcgtc gagtagctcg
     4441 tagcgttcgg ccgtcggagg atacgggatg ccgaggccgg tcgcctcgtc ctcgtagtcg
     4501 ccgacaccga taccaagcca ggcacgaccg ccggagagca cgtccagtgt cgtcaccgtc
     4561 ttgaccagca tggcggggtt gcggtaggac accggcgtcg ccagcgcgat caggtcggtg
     4621 cggttggtcc gggccgccag aaaggccagc gtcgaatagg cttcgagcac cggcccctcc
     4681 ggccccccgc ccgcgtatgg atgttgccag acatggtcca tgaccgcgat acgggcgaaa
     4741 ccggcctctt cggccgcctg cccgatgtcg gcaatcgtcg ttgcaagctg ttgcgtctcg
     4801 attggccagc cgaagtcgtt gatatgcagc cctaacttca agcctgccac ctccttccgt
     4861 gacaaatttc attgtataat acatacaaag aacgttgccc agagccactc cgcccgccgt
     4921 gccagcgacc acctggcggg gccgttcccc tgtcatcttt cgctgccggg tgccctctgg
     4981 gcggtggacg acaggggaac ggcgtcgaga tctgttctgt agaaa
//
LOCUS       DASUGJ010000102         3175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1820663, whole genome shotgun sequence.
ACCESSION   DASUGJ010000102 DASUGJ010000000
VERSION     DASUGJ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820663"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2065
                     /locus_tag="VFV93_02300"
     CDS             <1..2065
                     /locus_tag="VFV93_02300"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5164202.1"
                     /translation="VQTEAATVQSVDSTTEPLLVVNDLQTFFFGEGRVVRAVDGISYS
                     LQPGEVVGIVGESGSGKSVSSLSLMRLIESPPGRIVGGEIRFEGDDLLGMTDAEMRDI
                     RGNKISMIFQEPLTALNPVYTIGDQIMEPLVLHQGLKRPAARDRAIEMLQRVGVPSPA
                     ERIKAYPHQLSGGMRQRAMIAMALSCNPRLLIADEPTTALDVSIQAQILELMRELQRD
                     FGSSMIFITHDLGVVSEMCDRVIVMYAGRIVEQGSTRQIFTNPTHPYTWGLFDCLPKI
                     EERKGIRLTPIFGQPPNLANVPSGCKFHPRCPYAFERCTLEEPPLFEREDGQTVRCWL
                     YDNGADGSKQRVSEVRESSLTERPEQNDVLLDVRDLRKWFPITRGIFQRTVGQVQAVD
                     GLSFQLHRGETLGVVGESGCGKSTAGRVLLRLLDPTSGQVMFDGQDLAKLNHEGLRAM
                     RRRMQIIFQDPYSSLNPRRTVGSIVAQPLTLHKIARGKDVDERVSDILERVGLNPAHA
                     ERYPHEFSGGQRQRIGIARAIAANPDFIVCDEPVSALDVSIQAQIVTLLQDLQSDLSL
                     SYIFIAHDLSVVRSVSDRVAVMYLGKIVEIGANDQIYEDTLHPYTKALLSAVPKTDAS
                     GQNGSRIILRGDVPSPINPPSGCRFHTRCPYAFDACSKVEPPLIDAGNGHRVACHLYS
                     AVGES"
     gene            complement(2069..>3175)
                     /locus_tag="VFV93_02305"
     CDS             complement(2069..>3175)
                     /locus_tag="VFV93_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5164203.1"
                     /translation="NASSSVADAIAVRGSILAPTLNMAAADSDGEIATIGIGRWPDRR
                     EPAGLLDPAAFPPRYIPPSEMPIERNPARGWVASANNPIVGDDERYSLYGFYEPPYRI
                     RRISDILDSRPRHTVADMRALQLDQYSLHAAELTPFLLDLAGDALPAWARDDLTNWDF
                     QTPPESRATLLFQAFYRHWVRLSLAYRLPDAVVAKVMEGLDVGDVPMGFCDRLLRGEL
                     TEWWEGDLVSTVRLTVEESLQWLTEQLGPDHETWQWGNLHTVTWVHPFGQIVGPHQRF
                     VNVGPFAIGGERTTVWPSGFDAAHLFQVTGGPSMRLLADLRRPERTWVTNTLGQNGRP
                     FSRHYRDQTLDFIEGRSHSLWGQPLRTHVIIEPS"
BASE COUNT          557 a         1062 c         1012 g          544 t
ORIGIN      
        1 ggtgcagacc gaagccgcca ctgtccagtc cgtcgacagc acaaccgagc cgctgctggt
       61 tgtcaacgac ttgcagacct tcttcttcgg cgagggtcgg gtcgtccggg cggtcgatgg
      121 catcagctac tcgctgcagc cgggcgaggt cgtcggtatc gtcggcgaat cgggaagcgg
      181 gaagagcgtc agctccctct cgttgatgcg cttgatcgaa agcccgccgg gccggatcgt
      241 cggcggtgag atccgcttcg agggtgacga tctgctgggg atgaccgacg ctgagatgcg
      301 cgacattcgc ggcaacaaga tctcgatgat ctttcaggaa ccgctgacgg ccctgaaccc
      361 ggtctacaca atcggtgatc agatcatgga gccgctggtc ctgcatcagg ggctcaagcg
      421 gccggcggca cgcgaccgcg ccatcgagat gctccagcgc gtcggcgtgc cgtcaccggc
      481 cgaacggatc aaggcctacc cgcatcaact ctccggcggg atgcgccagc gagcgatgat
      541 cgcgatggcg ctctcgtgca acccccggct gctgatcgcc gatgagccca cgacggcgct
      601 cgatgtctcg attcaggcgc agatccttga actgatgcgc gagctccaac gcgatttcgg
      661 ctcgtcgatg atcttcatca cccacgacct cggcgtcgtc agcgagatgt gcgatcgcgt
      721 catcgtcatg tacgccggcc ggatcgtcga acagggctcc acgcgccaga tcttcaccaa
      781 cccgacccat ccatacacct gggggctgtt tgactgcctg ccgaagatcg aagagcgcaa
      841 gggcatccgg ttaacaccga tcttcggcca gccgccgaac ctcgccaacg tccccagcgg
      901 ctgcaagttc cacccgcgct gcccgtacgc gttcgaacgc tgcacgctgg aggaaccgcc
      961 cctcttcgag cgcgaggatg gacagacagt gcggtgctgg ctgtacgaca acggggcaga
     1021 cgggtcgaag cagcgggtca gcgaagtccg cgagtccagc ctgaccgagc gaccagaaca
     1081 aaacgacgtc ctgctcgacg tgcgcgacct gcgcaagtgg ttcccaatta cgcgcggcat
     1141 cttccagcgg acggtcggtc aggtccaggc cgtcgatggt ctcagcttcc agttgcaccg
     1201 gggcgagacg ctcggcgtcg tcggcgagtc cggctgtggc aagagcacgg ccggccgcgt
     1261 tctcctgcgc ttgctcgatc ctaccagcgg ccaggtcatg tttgacggtc aggatctggc
     1321 gaagctgaac cacgagggac tgcgcgcgat gcgcaggcgg atgcagatca tctttcagga
     1381 cccgtactca tcgctcaacc cacggcgtac cgttggctcg atcgtcgccc agccgctaac
     1441 gttgcacaag atcgcgcgcg gcaaggatgt cgacgagcgg gtcagcgaca tcctggagcg
     1501 cgttggcctc aacccggccc acgccgaacg ctatccgcac gagttctccg gcggccagcg
     1561 ccagcgaatc gggatcgccc gggcgattgc cgccaatccc gacttcatcg tctgcgacga
     1621 gccggtttcg gcgttggatg tctcgattca ggcccagatc gtcactctgc tgcaggattt
     1681 gcagagcgac ctgagtctgt cctacatctt catcgcccac gacctgtcgg tcgtccgctc
     1741 ggtcagcgat cgggtggcgg tgatgtacct aggcaaaatc gttgagatcg gcgccaatga
     1801 ccagatctac gaggacacac ttcatccgta caccaaggcg ctcctttcgg ccgtcccgaa
     1861 gacggacgcg agcggccaga acgggagccg gatcatcctg cgcggcgatg tccccagccc
     1921 gatcaacccg ccctccggct gccgcttcca tacccgctgc ccgtacgcat tcgacgcttg
     1981 ctcgaaggtc gagccgccgc tgatcgacgc cggcaacgga caccgtgtcg cctgccacct
     2041 ctacagcgcg gttggcgagt cgtaagcgtc aggatggctc gatgatgaca tgcgtccgca
     2101 ggggctggcc ccagagcgag tgcgaccgtc cctcgatgaa gtccagcgtc tggtcgcgat
     2161 agtggcggct gaacggcctg ccgttctggc cgagcgtgtt ggtcacccag gtcctctccg
     2221 gccggcgcag atcagccagc agccgcatcg acggcccgcc ggtcacctga aacagatggg
     2281 ccgcgtcgaa gccggacggc cagacggtcg tgcgctcgcc gccgatggcg aatggcccga
     2341 cattgacgaa gcgctggtgc gggcccacga tctgcccgaa cggatggacc caggtcaccg
     2401 tatgcaagtt tccccattgc cacgtttcgt ggtccggtcc caactgctcg gtgagccatt
     2461 gtagtgattc ttcgaccgtg agcctgacgg ttgacaccaa atcgccctcc caccactccg
     2521 tcagttctcc cctcaggagc cggtcgcaga atcccatcgg cacatcgcca acatccagcc
     2581 cctccatcac cttcgcaaca acggcgtcgg gcagtcgata cgccagcgac agacgcaccc
     2641 agtgccggta gaacgcctgg aacagcagcg tcgcccggct ctccggtggc gtctggaagt
     2701 cccagttcgt caggtcgtcc cgcgcccagg cgggcagcgc gtctccggcg aggtccagca
     2761 ggaacggcgt cagttcggcc gcatgtagcg agtactggtc gagctgaagc gcccgcatgt
     2821 cggcaaccgt gtgcctgggc cgcgagtcga ggatgtcgct gatgcggcgg atgcgatacg
     2881 gcggctcgta gaagccgtac aggctgtatc gctcgtcatc tccaacgatc gggttattgg
     2941 cgctcgcgac ccagccgcgc gccggattgc gttcgatcgg catctcgctg ggcgggatgt
     3001 agcggggcgg gaaggcggct ggatcgagca gcccggccgg ctcccgccga tcgggccagc
     3061 ggccgatgcc gatcgtggcg atctcaccgt cggagtcggc ggcggccatg ttgagtgtcg
     3121 gtgcgaggat cgacccccgg acggcgatcg cgtccgccac cgaggagctg gcgtt
//
LOCUS       DASUGJ010000103         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_708435, whole genome shotgun sequence.
ACCESSION   DASUGJ010000103 DASUGJ010000000
VERSION     DASUGJ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708435"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1702
                     /locus_tag="VFV93_02310"
     CDS             200..1702
                     /locus_tag="VFV93_02310"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5164204.1"
                     /translation="MTATAIETYQLLIDGQMVDAASGETFETVSPATNRPVGKIAKAG
                     REDVDRAVAAARKAFDDGRWSRMAPAERAKRMRKAADLIRERVDELARLETLNCGKII
                     VESRADVLNTAACLDYYASLTGQMWGETIPMNGPLLDYTLREPVGVCAQIIPWNFPLL
                     MAAWKIGPALAMGNTIVLKPASNTPITAVILGQICLEAGIPDGVLNVLTGPGAKIGSA
                     LCEHPDVDKVAFTGETETGRGIIRQSAETIKKVSLELGGKSPNIVFDDADLDEAVNGS
                     LFAVFTNAGQRCTARTRLFLHESIHDKFMADFVAKTAQIKIGDPLEWDTQMGPVISKT
                     QCERVLSYVERARDGGADLAHGGRRITEDPFVDGNFVTPTVFDRVTNDMTIAQEEVFG
                     PVLAVIPFKDDAEVVQMANNTIYGLAATIWTNDVKRAHTMAANIRAGNVSINYPTVNP
                     PEAPFGGYKQSGTGRELSRHALDLYTQVKNVVVNLNPQPFDWYGNWPSKP"
     gene            complement(1767..2531)
                     /locus_tag="VFV93_02315"
     CDS             complement(1767..2531)
                     /locus_tag="VFV93_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5164205.1"
                     /translation="MQITFLGTGTSNGIPVIGCTCPVCTSPDPRDKRSRTSAVVEVDG
                     RTLLIDTSPELRLQAIANRLTHVDAVLFTHAHADHIAGFDDLRSFNFLSQAPLDVHAD
                     VFTSDLIRERFAYAFEKPLPFFGGKPDLNLHAFDGPFAAAGVEVVPFRVGHGRWTVSG
                     FRFGLLVYLTDAKIVPPAAIDAMRGAEVLVINALRDRPHPVHLSVGEALEIIAEVRPR
                     RAYLTHLSHDVGHAELSARLPDEVQVAYDGLVVEVD"
BASE COUNT          542 a          916 c          868 g          428 t
ORIGIN      
        1 ttccatctcg tcctcttcat cgagccgcgt cgattcagcc tgcgccatgc aggcagcgac
       61 gtccaccacg gaaacatcaa gtatagccgg gaaacagccg ccaccgaagc agggcaggct
      121 gccggtcagg cactatactc acgactgaca ggtaatcgca ggctttgcgc cagcaacgat
      181 gaccatgggg agggactgga tgacggcgac tgcaatcgag acatatcaac tcctgataga
      241 cgggcagatg gtggacgcag ccagcggcga gacgttcgag accgtctcac cagccaccaa
      301 ccgtccggtc gggaagatcg ccaaggccgg gcgagaggac gtggaccgcg ccgtcgcggc
      361 cgcccggaag gcgttcgacg acggacgctg gtcgcggatg gcgccggccg agcgcgcgaa
      421 gcgcatgcgc aaggcggccg acctgatccg cgagcgggtc gatgagctgg cccggcttga
      481 gaccctcaac tgcggcaaga tcatcgtcga gtcgcgggcc gacgtcctca acacggccgc
      541 ctgcctcgac tactacgcca gcctgaccgg tcagatgtgg ggcgagacga tcccgatgaa
      601 tggcccgctg ctggactaca cgctgcgcga gccggtcggc gtctgcgcgc agatcatccc
      661 atggaacttc ccgctgctga tggccgcctg gaaaatcggc ccggccctgg cgatgggcaa
      721 cacgatcgtc ctcaagccgg ccagcaacac gccgatcacg gccgtcatcc tcggccagat
      781 ctgcctcgaa gccggcattc ctgacggcgt cctcaacgtc ctgaccggcc ccggcgcaaa
      841 gatcggctcg gcgctctgcg agcatccgga tgtcgacaag gtcgcgttca ccggcgagac
      901 cgagaccggg cgcggcatca tccgccagtc ggccgagacg atcaagaagg tgtcgctgga
      961 gctgggcggc aagagcccca acatcgtctt cgacgacgcg gacctcgacg aggccgtcaa
     1021 cggctcgctg ttcgccgtct tcaccaacgc cggccagcgc tgcacggccc ggacacgtct
     1081 cttcctccac gagtcgatcc acgacaagtt catggccgac ttcgtcgcca agacggcgca
     1141 gatcaagatc ggcgacccgc tcgagtggga cacccagatg gggccggtca tctcgaagac
     1201 gcagtgtgag cgcgtcctct cctacgtcga gcgtgcccgc gatgggggcg ccgacctggc
     1261 ccacggcggt cgccggatca cggaagatcc gttcgtcgac ggcaacttcg tcacgccgac
     1321 cgttttcgac cgtgtcacca acgacatgac gatcgcccag gaagaggtct tcgggccggt
     1381 cctggccgtc atcccgttca aggatgacgc cgaggtcgtc cagatggcca acaacacgat
     1441 ttacggcctg gccgcgacga tttggaccaa cgacgtcaag cgcgcccaca cgatggccgc
     1501 caacatccgc gccggcaacg tctcgatcaa ctacccgacc gtcaacccgc ccgaagcccc
     1561 cttcggcggc tacaagcagt ccggcacggg ccgcgagcta tcgcgccacg cgctcgacct
     1621 ctacacccag gtcaagaacg tcgtcgtcaa cctcaacccg cagccattcg actggtacgg
     1681 caactggccg tccaagccgt aacagatgac gacctctccg cgtaggggcg atggccgcag
     1741 cctcgccctt tgcgcggaaa tggtgcctag tcgacctcga ccaccagccc atcgtaggcg
     1801 acctggacct catccggcag gcgcgcgctg agttcggcgt ggccgacgtc gtgggagaga
     1861 tgcgtcaggt aggcgcgacg cggccggacc tcggcgatga tctcgagtgc ctccccaacg
     1921 gagagatgga ccgggtgggg acgatcccgc agggcgttga tcaccagcac ctcggcgcct
     1981 cgcatcgcgt cgatcgcggc cggtgggacg atcttcgcgt cggtcaggta gaccaacaga
     2041 ccaaagcgaa accccgacac cgtccaacgc ccatgcccga cccggaacgg cacgacctcg
     2101 acgccggccg ccgcaaacgg gccgtcgaat gcgtgcaggt tcagatccgg cttgccgccg
     2161 aagaacggca gcggcttctc gaaggcgtag gcgaagcgct cgcggatcag atcgcttgtg
     2221 aacacatcgg catggacatc gagcggcgcc tggctgagga agttgaagct gcgcagatcg
     2281 tcgaacccgg cgatatggtc ggcatgcgcg tgagtgaaga ggaccgcatc gacatgcgtg
     2341 agtctgttgg cgattgcctg caggcgcaac tccggcgagg tgtcgatcag gagtgtccga
     2401 ccgtcaacct cgacaactgc cgaggttcgc gaccgcttgt cacgcgggtc aggtgatgta
     2461 cagaccggac atgtgcagcc gataacgggg ataccgttgc tggttccggt gccgagaaac
     2521 gtgatctgca tatgctcctg acagtcattg agccctcgcg tgttcctgca acgcgtccgt
     2581 caagcggcaa tcaagtatac gtttggccgg aaccactcgc ggttggggtc atggttggca
     2641 tgtgattgaa agacggtacc aatagggtac tccgacggca ttgtcgcgcc aatctggctc
     2701 gatatgatga tagtagccaa cagggctgcc aggtcgctcc ggccggccac ctcg
//
LOCUS       DASUGJ010000104         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1403197, whole genome shotgun sequence.
ACCESSION   DASUGJ010000104 DASUGJ010000000
VERSION     DASUGJ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403197"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFV93_02320"
     CDS             complement(<1..576)
                     /locus_tag="VFV93_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5164206.1"
                     /translation="MVTVAQPAGKTRAESLAAPAEAQARVPRVSLGAAGMVFATIAML
                     LTALAMYMPQSRFDVWTINRIQEIDAPYLHAIITVVSTLTSSIYAIAFWAIMLVALSV
                     SRRWLPALAMLVMPAGGVINEFIGEIIVGRTRPDASVVQRTVPDIQAASFPSGHVMGA
                     VMFYGVIFFLARKIQNSVLRLTVQTFSVAIVA"
     gene            932..1633
                     /locus_tag="VFV93_02325"
     CDS             932..1633
                     /locus_tag="VFV93_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5164207.1"
                     /translation="MFRNLRQSLGLTGLLILFIIAVSVALTIQLSIEASETATLSGDV
                     AVSTWVQRTSLPGVGYLVEILNWIGQPIPLALLTGLVAVGLMSRRRYAEALLILPATG
                     THLVNAIIKNVVQSPRPTGQQVRIQDHAAGFGFPSGHTMAIVVFCGVIAYLAWRLIDR
                     KRYQYAVHAVVALAIVGIGFSRIYSGAHWPSDVLGGYLWGAFYTGVLVLLFHRLQPLN
                     AGERVPATRPRPETA"
     gene            complement(1640..>2104)
                     /locus_tag="VFV93_02330"
     CDS             complement(1640..>2104)
                     /locus_tag="VFV93_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5164208.1"
                     /translation="LDHLPDTQLPGDYVIRNLRGEVDVAARVAAHRDAFAPSKMTEEK
                     HRAVMASPTYRPELDLIVEAPDGSVAAYCLVWLDDANAHGVFEPVGCHSAHRRRGLTK
                     EVVFEGMRRLRDRGARTASVVSHTSSVAANRLYASAGFTDADTVREWVKPLR"
BASE COUNT          370 a          703 c          657 g          374 t
ORIGIN      
        1 ggcaacgatc gcgaccgaaa aggtctggac cgtcagccgc aggacgctgt tctggatctt
       61 tcgagccagg aagaagatca cgccgtagaa catgacggca cccatgacgt ggccgctcgg
      121 gaacgaggcc gcctgaatgt cgggaaccgt ccgctggacg acgctggcgt ccggccgagt
      181 gcggccgacg atgatctcgc cgatgaactc gttgatgacg ccgccggccg gcatcaccag
      241 catcgcgagt gccggcagcc agcggcggct gacgctcagc gccaccagca tgatcgccca
      301 gaacgcgatc gcatagatcg agctggtcag cgtgctgacg acggtgatga tcgcgtgcag
      361 gtacggcgcg tcgatctcct ggatacgatt gatcgtccag acatcgaacc gactctgcgg
      421 catgtacatc gcgagcgctg tgaggagcat cgcgatcgtc gcgaacacca tgcctgccgc
      481 acccagcgaa acgcgtggga cgcgagcctg cgcttctgcc ggcgccgcga gggactcagc
      541 cctcgtcttc ccggccggct gtgcaacggt aaccatcgta tctacccccc tttgggtaga
      601 gcgcctcgct gaagccatcc gtgcttccgg cgcccactga acgtcatcgt ccagttattc
      661 gatgataggc agttgccggg atcacaacat cggccaactg attgaattcc gtctccgacg
      721 ggtcagtcgg tcagcctgtc cattggtact ggggttggtc agtctttggc atcacaagac
      781 cgtcacaatc gggacgattc atggcgcccg ccgctcgtag caggggcacg ccgaatgcta
      841 agatgccgtg gctgcggacg cgggtcgcgg cccgattcca gcagcgggac cgtggggatt
      901 tgatcgtctg aacgaaacga gtcatccttc gatgtttcgc aacctgcgcc agtcgctcgg
      961 actgacgggt ctcctgatcc tgttcatcat cgcggtctca gtcgccctca ctatccagct
     1021 ctcgatcgaa gcctcggaaa ccgccacgct ctccggcgat gtcgcggtca gcacctgggt
     1081 gcagcgcact tcgttgcccg gtgtcggcta tctggtcgag atcctgaatt ggatcggtca
     1141 gccgatcccg ctggcgctgc taaccggtct ggtcgccgtc gggctgatgt cacggcggcg
     1201 ctacgcggag gcgctcctca tcctgccggc gaccggcacc cacctggtca acgcgatcat
     1261 caagaacgtc gtccagagtc cccgcccgac cggccagcag gtccgcatcc aggaccacgc
     1321 ggccggcttc ggctttccaa gcgggcatac gatggcgatc gtcgtcttct gcggcgtgat
     1381 cgcctacctc gcctggcggc tgatcgatcg gaagcgctac cagtacgcgg tccacgccgt
     1441 cgtcgcgctg gcaatcgtcg ggatcggctt cagccggatc tactccggcg cccactggcc
     1501 gtcggacgtg ctcggcggct acctctgggg cgcgttctac acgggagtgc tggtgctgct
     1561 cttccatcgc ctgcaaccac tcaacgccgg cgaacgcgtg ccagccaccc gaccacgtcc
     1621 tgagacggca tgatcaccat cagcgcagcg gcttgaccca ttcgcgaacg gtgtcggcgt
     1681 cggtgaatcc ggccgaggcg tacagccggt ttgcggcgac cgaggacgtg tggctgacaa
     1741 cagaggcggt tcgcgcgccg cgatcgcgca agcggcgcat cccctcgaac acgacctcct
     1801 tcgtcagccc gcgccgccgg tgtgccgagt gacatccaac cggctcgaag acgccgtgtg
     1861 cgttcgcgtc atccagccag acgaggcagt aggccgcaac cgagccatcg ggagcctcga
     1921 cgatcaggtc cagctccgga cgataggtcg ggctcgccat caccgcccgg tgcttctcct
     1981 cggtcatctt cgatggagca aacgcatcgc gatgggcggc aacgcgcgcg gcgacatcga
     2041 cctcgccacg caggttgcga atgacataat ctccaggcag ctgagtgtcg ggtagatgat
     2101 cgag
//
LOCUS       DASUGJ010000105         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_153246, whole genome shotgun sequence.
ACCESSION   DASUGJ010000105 DASUGJ010000000
VERSION     DASUGJ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153246"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VFV93_02335"
     CDS             complement(<1..1017)
                     /locus_tag="VFV93_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5164209.1"
                     /translation="MTFTLGFILIGLLLVGMALAGSLLRRLPISAAMIYVVVGLILGP
                     IGIGLIELDPIEDSGFVERVTEIAVIISLFTAGLKLRVSLLDPIWRVSLRLALVAMLA
                     TIGLVAVVATVALDMPIGLAILLGAVLAPTDPVLASDVQVRTPGDRDLLRFSLTSEAG
                     MNDGTAFPFVLLGLGLLHLHELGDSGWRWLTLDVFWAVIVGLGIGTVLGTLVGRFVIY
                     LRRSHSEAIGLDDFLALGLIALSYGVALLVDAYGFLAVFAAGLAVRRIEARSSSSEEV
                     DEILLPASIEDEAPDEASHPETAPAYMTAAVLHFNEQVERIGTTAIVVLVGAMLSTRF
                     LHADA"
     gene            complement(1036..2754)
                     /locus_tag="VFV93_02340"
     CDS             complement(1036..2754)
                     /locus_tag="VFV93_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5164210.1"
                     /translation="MEDSHVILNIAVALAVAFVGGVVARRLRLPVIVGYLIAGLAIGP
                     STPGFDADVESVRVLAELGVAFLMFSLGVEFSLQELLQVRRIALGAGSLQVVLTTLFG
                     MAVASLLGWSWTQAIVFGMTVALSSSVVALKLLGLRGETATRHGRVTAGIAVFQDLSL
                     VPMLIVLPVLANDSGNLLLGVLRSVGIAIVVIGLVIFLGLRIVPRLLEVIAETASREL
                     FLLAVVVIALGTAITTQEAGLSIALGAFLAGMIVSESDFSHQVLADIIPLREAFATLF
                     FVSVGMLLDLSYVSHHLGVVVLITIVVLVGKMLILTGVVRGFGLPLTSAVLVGVLLPQ
                     IGEVSFILAGEGFDRELLDRGGYNLILAVSVGTLLISPMLDAAAPSALRYGRRFSKSL
                     FVELDTSLLEGAPPTRHTIVCGYGRLGVVLVDAMRRRGFSCVVVDNDPASVREAIKSG
                     VPAIYGDAGNPEILSRLGISRARVLVVAISDPLAAEAAVESGRRANQRLDIVARARSR
                     EQMHRMRELGANEVIQPEFEAGLELIRHVMHVHGLDQRQVGAIVQRRREMYYTVEERD
                     LRDREA"
     gene            complement(2810..>3214)
                     /locus_tag="VFV93_02345"
     CDS             complement(2810..>3214)
                     /locus_tag="VFV93_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX5164211.1"
                     /translation="DLELLRSQAFAALALSLPDGALQNTIMWYRVAEGTLRMIAPAAS
                     AKARALRRSPQVAIVVHAPDNSYDYLEIRGHAEVIADDAAARAELREIAKRYVGERAG
                     AYVDGLSDEPRVMIRVTPERVRRHRSAPLPTT"
BASE COUNT          710 a         1090 c          960 g          454 t
ORIGIN      
        1 cgcgtcggca tgaagaaagc gagtcgacaa catcgctccc accagcacaa cgatggcagt
       61 cgtaccaatc cgctcgacct gctcgttgaa gtggaggacg gcggctgtca tgtaggctgg
      121 ggcggtctcc gggtggctgg cttcgtcggg tgcctcgtct tcaatcgatg ccggcaacag
      181 gatctcgtcg acctcctcgc tgctcgacga tcgcgcctca atccgccgca ctgcgaggcc
      241 ggcagcgaag acggccagga aaccataggc gtcgaccagc agcgccacgc catatgacag
      301 cgcgatcagc ccaagcgcta ggaagtcatc gagcccgatc gcctcgctgt gtgagcggcg
      361 caggtaaatc acgaaccggc ccacgagcgt gccgagaact gtgccgatac cgaggccgac
      421 aatgacggcc cagaagacat cgagagtcag ccagcgccag cctgaatcac ccagctcatg
      481 cagatggagc agccccaggc cgagcagcac gaacgggaag gcggtgccgt cgttcatgcc
      541 ggcctcgctc gtcaggctga agcgcagcag atcgcgatct cctggcgtgc gtacctggac
      601 atcggacgcc aggaccgggt cggtcggggc caggactgct ccgagtagaa tcgccaaccc
      661 gattggcata tcgagcgcaa ctgtcgcaac gacagcaacc agtccaatcg tagccagcat
      721 cgcaaccagc gccagccgta gagacacgcg ccagatcgga tcgaggagtg acacgcggag
      781 cttcaggccc gccgtgaaga gcgagatgat gacggcgatt tcagtgactc gctcgacgaa
      841 cccactgtcc tcgatcgggt cgagctcgat cagaccaatt ccgatcggac ccaggatcag
      901 accgacgacg acatagatca tcgccgccga aatcggcaac ctgcgcagta gcgagccggc
      961 cagcgccatc ccaaccagca gcaacccgat caggatgaaa ccgagggtga atgtcatcgg
     1021 cagccaccac gcgcatcacg cttcacgatc gcgtaaatct cgttcctcga cggtgtagta
     1081 catctcccgc cggcgctgga cgatcgcacc aacctgccgc tggtcgaggc cgtggacgtg
     1141 catgacatgc cggatcagtt caagtcccgc ctcgaactcc ggctggatga cctcgttcgc
     1201 gcccagttcg cgcatccggt gcatctgctc gcgactgcgc gcgcgggcga cgatatccag
     1261 ccgctggttc gcccgccggc cggactcaac ggctgcctcc gccgccagcg ggtctgaaat
     1321 ggcaacgacc agcacgcgcg cacgactgat gccaagccgg ctgagaattt ccgggttacc
     1381 ggcgtcccca tagatcgccg gcacgccact cttgatcgcc tcccgaactg acgcggggtc
     1441 gttatcaacg acgacgcacg agaacccgcg tcgtcgcatg gcatcaacca gcacaacacc
     1501 gagccgccca tagccacaga cgatcgtgtg gcgagtcggg ggtgcccctt ccagcagcga
     1561 cgtgtccagc tcgacgaaca acgacttgct gaaacgccgc ccgtagcgca acgcggacgg
     1621 cgcggccgcg tccagcatcg gggaaatcag cagcgtgccg acggagacgg ccagaatcag
     1681 gttgtacccg ccgcggtcga gcaactcgcg atcgaacccc tcaccggcca ggatgaacga
     1741 cacctcaccg atctgcggca gcagcacgcc gacaaggacc gcgctcgtca acggcaaccc
     1801 gaatccacga acgacgccgg tcaggatcag cattttgccg accagcacga cgatggtgat
     1861 gaggacgacg acaccgaggt gatggctgac gtacgagagg tcgagcagca tcccgactga
     1921 gacgaagaac aacgtcgcga acgcctcacg caacgggatg atgtcggcca gcacctgatg
     1981 actgaaatcc gactcggaca cgatcatacc ggccaggaac gctccgagcg cgattgagag
     2041 accagcctcc tgagtcgtga ttgccgtgcc caatgcgatg acaacaaccg ccagcagaaa
     2101 gagctcacgc gaggctgtct cggcaatcac ctccagcaac cgcggcacaa tccgcaagcc
     2161 caggaagatg accagtccaa taacgacgat cgcaatgcca accgaacgga gcacgccgag
     2221 gaggagattg ccgctgtcat tggcaaggac cggcaggacg atcagcatcg gcaccagtga
     2281 caggtcctgg aagacagcga tcccggctgt gacgcgtccg tgcctggtcg ccgtctcgcc
     2341 gcgcagcccc aacaacttca atgcaacgac cgacgaggag agcgcgacgg tcatcccgaa
     2401 cacgatcgcc tgcgtccatg accagcccag cagcgatgct accgccatgc cgaacagcgt
     2461 cgtcagcacg acctggagcg agccggcccc gagcgcaatc cggcgaacct ggagcagctc
     2521 ctgcagggag aactcgaccc cgagtgagaa catcagaaag gcgacgccca gctccgcaag
     2581 cacgcggacc gattccacat cggcgtcgaa ccccggcgtg gatggcccaa tcgccaaccc
     2641 cgcgatcagg tagccaacga tgaccggcaa ccgcaaacgg cgcgcgacga cgccgccgac
     2701 gaaggccaca gccagcgcca cggcgatatt caggatgacg tggctgtcct ccaatggctg
     2761 ggcccttctg tcggccagtc acggatcggt tacgggtaga ggcgagtcgc tatgtcgtcg
     2821 gcagcggcgc tgaccgatgc cttcgcaccc gctcgggcgt cacgcgaatc atcacccttg
     2881 gctcgtccga cagcccatcg acgtacgcgc cggcacgctc gccgacgtag cgcttcgcga
     2941 tttcacgaag ttccgcgcgg gccgccgcat cgtcggcgat aacctcggcg tgtccccgaa
     3001 tttcgaggta gtcatacgag ttgtcgggag catggactac gatcgcaacc tgcggtgatc
     3061 gacgaagggc gcgcgctttg gccgacgccg ccggtgcgat catccgcaga gtaccctctg
     3121 caacacggta ccacataatc gtgttttgca gggcaccgtc cggcaacgac agcgccagtg
     3181 ccgcaaatgc ttgcgaccgc aacagctcca gatc
//
LOCUS       DASUGJ010000106         5282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_153253, whole genome shotgun sequence.
ACCESSION   DASUGJ010000106 DASUGJ010000000
VERSION     DASUGJ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153253"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VFV93_02350"
     CDS             <1..871
                     /locus_tag="VFV93_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164212.1"
                     /translation="ILHLIPGDPARMLLGDMGGGAAAGDTSDEAYENLREELGLNDPY
                     IVQYGRYLTDALQGDFGRSFQTNRDVRDSIFEVLPYTLELTVAGLGFAILFGGVLGII
                     AALNRNTWIDSLTMVFSLFGLAMPSFWLGFILIQFFALRWSIFPSTGSDGWKTLVLPA
                     LTLGLVASGTVARLVRSTMLEVLRQEYVTTARSKGLAERVVILRHALRNALIPVVTIV
                     GLQFGGLLSGSVIIETVFARRGLGRLAVEAVLSRDFPMVQGTILFAAVGYVFVNLLID
                     LSYAWLDPRIKYR"
     gene            883..1785
                     /locus_tag="VFV93_02355"
     CDS             883..1785
                     /locus_tag="VFV93_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164213.1"
                     /translation="MERDLTAGAGAPTLSLGTVKPRSRSREILRGLVRNKGALAGLII
                     LIVMILGAVFAPLITTYDPIKIDPPSRTLPPSRDHWLGTDAFGRDIYTRIVYGGRVSL
                     PVGLIAVAIAASVGALLGLIAGYYGRWADGIIMRLIDILLAFPNIMLALIIVAILGPN
                     IRNVMIAVGVGAIPAYTRLIRGQVLSARELLYVEAAKVIGVPTSKIMYRHILPNVVSP
                     VIVLGTLSVGTAILAAAGLSFLGLGAQPPRPEWGSMLADGRQFLRSHWWVATMPGMAI
                     ALTVLSINMFGDGLRDILDPRIRS"
     gene            1912..3606
                     /gene="ggt"
                     /locus_tag="VFV93_02360"
     CDS             1912..3606
                     /gene="ggt"
                     /locus_tag="VFV93_02360"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5164214.1"
                     /translation="MANQTTLRVTRDLATSRNSMVAAKHPAAVEAALDILDKGGNAVD
                     AAATAAFAVGVVEPWMSGIGGVGFMTIQMAGKEPVVIDYFGRAPGAATPDMYELTGEE
                     RSVVGFGGVKDQANAYGALSCTVPGMVAGLAYAVEHYGKKELAEIVAPAARLADEGFE
                     VNWYNGMLLSSQQKTLQRDAESARIFLNNGTPPAPLFGQPNPRIRQPELAGTLRKIAA
                     QGRDGFYKGEVAEKIASHLQSLGGIMTADDLARYEPVVNKPIVIPYQGYELVLLPFQG
                     GGITLAETFNILDGLNIRATGHNTAQTLHSIDQASKSAFADRFAFIGDPDYVDIDWDK
                     LASKAYADQRRAALDPNRATVPQPGDGIRRGSGLKPEMAAQMDGGCTTHLSVVDKDGN
                     MVSVTQTLTLIFGSVVTIPGVGVIMNDTMNLFEPIPGRANSIASWKRPASNMAHVVAT
                     KDGVPVLAVGAPGGRRIIDTCLQMTLNVLDFDMDIAAACGAPLIDCSGAELLADDRIP
                     QATRDRLRAMGHDVVDAEVSFSPRAFASPTGVTVDPETGLRGGGADPFGIGIAAGR"
     gene            complement(3668..4606)
                     /locus_tag="VFV93_02365"
     CDS             complement(3668..4606)
                     /locus_tag="VFV93_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5164215.1"
                     /translation="MTRQRPFRFACGAFTASSSQAWSEFARRVEALGYHAFLMPDHFG
                     QNLAPAVGLLAAAYATTSLRIGCTVFDNDFRHPAILANETATLDMLSDGRFEFGIGAG
                     WVKREYEQTGIPFDAPGTRVDRMIEAVSIIKRLWSGEEVHHCGAHYTITGLTDCVRPV
                     QQPHPPVFIGGGGKRLLTFAAQEADTVGILARARPAGGLGFGNDETDDAIARKVDWVR
                     SAAGARFERLELAMLIWDVIITDNRRAAAETIASGARGVTADEVLASPYYLLGSVDAI
                     VDQILGLRERFGISHFSVFPEDMEFFAPVVARLRGK"
     gene            4796..>5282
                     /locus_tag="VFV93_02370"
     CDS             4796..>5282
                     /locus_tag="VFV93_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5164216.1"
                     /translation="MSGQPTEYLSMRRIGDAEMTLILEGAGPYPVELNVPEDVWRPAV
                     PDADANGNVLLCSGGAYVRIGDASIVIDPGLDDPGTPTSAATELVFKDWSFTPGFQAA
                     LDELGVDNDAVTHVIITHAHFDHCLGVAVERDGKLVPRYPNARYLLDRADWDLHFTPD
                     GE"
BASE COUNT          905 a         1823 c         1660 g          894 t
ORIGIN      
        1 catcctccat ctgatccctg gtgaccccgc ccggatgctg ctcggtgaca tgggtggcgg
       61 cgctgccgcc ggcgacacgt cagacgaagc ctatgagaac ctgcgcgaag agctcgggct
      121 gaacgatccc tacatcgtgc aatacggcag gtacctgacc gacgcgctgc aaggtgactt
      181 cggccgatcg ttccagacca atcgcgacgt tcgtgactcg atcttcgagg tgctgccgta
      241 taccctggaa ctgacggtcg ccgggcttgg cttcgcgatc ctgttcggcg gcgtgctcgg
      301 catcatcgcc gcactcaacc gcaacacctg gatcgacagc ctgacgatgg tcttctcgct
      361 cttcgggctg gcgatgccca gcttctggtt agggttcatc ctgatccagt tcttcgccct
      421 gcgctggtcg atcttcccga gtaccggttc ggacggctgg aagacgctcg tcctgccggc
      481 cctcacgctc gggctggttg cgtcgggaac cgtcgcgcgg ctcgtccgtt cgacgatgct
      541 cgaagtcctg cgccaggagt acgtcacgac cgcgcgctcg aaggggctcg ccgagcgcgt
      601 cgtcatcctg cggcacgcgc tgcgcaacgc gctgatcccg gtcgtgacga tcgtcggcct
      661 ccagttcggc gggttgctct ccggctccgt catcatcgaa accgtcttcg cgcggcgcgg
      721 cctcggccgg ctggccgtcg aggctgtcct gagccgggac ttcccgatgg tccaggggac
      781 gatcctgttt gccgccgtcg ggtacgtgtt cgtcaacctg ctgatcgacc tgtcgtatgc
      841 gtggctcgat ccgcgtatca agtaccgtta ggagtgcccg ctttggaacg tgaccttaca
      901 gccggcgctg gcgctccgac gctctcgctc ggtacggtca agccacgcag ccgcagccgg
      961 gagatcctgc ggggtctggt gcgcaacaag ggcgcgctgg ccgggctgat catcctgatc
     1021 gtgatgatcc tcggcgccgt cttcgcgccg ctgatcacca cctatgaccc gatcaaaatc
     1081 gacccaccga gccggaccct gccacccagc cgcgaccact ggctcggaac cgacgccttc
     1141 ggccgcgata tctacacgcg catcgtctat ggcgggcggg tatcgctccc ggtcggtctg
     1201 atcgccgtcg cgatcgcggc cagcgtcggc gcgctgctgg gcctgatcgc cggctactac
     1261 ggccgctggg ccgacgggat catcatgcgg ctgatcgaca tcctgctcgc cttcccgaac
     1321 atcatgctgg cgctgatcat cgtcgccatc ctcggtccca acatccgcaa cgtgatgatc
     1381 gcggttggcg tcggcgcgat cccggcctac acccggctga ttcgggggca ggtactgtcc
     1441 gcccgcgagc tgctctacgt cgaagccgcc aaggtcatcg gcgtcccgac atccaagatc
     1501 atgtaccgcc acatcctgcc caatgtcgtc agcccggtca tcgtcctcgg aacgctctcg
     1561 gtcggcacgg cgatcctggc agccgccggc ctctcgttcc tcggcctcgg cgcgcaaccg
     1621 ccccgtcctg agtggggctc gatgctggcc gacggccgcc agttcctgcg cagccactgg
     1681 tgggtggcaa cgatgcccgg catggccatc gcgctgaccg tcctctccat caacatgttc
     1741 ggcgacggcc tgcgggatat tctcgatcca cggattcggt cctaggcgtg acgcgtgatg
     1801 cgtaacgcga aggctgaatg caccgcgtca cgcgtcacgc gaaggctgaa tgcaccgcgt
     1861 cacgcgtcac gcgtcacgaa cacaaacggt aactgagggg agcacacaac gatggccaac
     1921 caaacaaccc tgcgcgtcac gcgcgatctg gcgacatcac gcaactcgat ggtcgcggcc
     1981 aagcatccgg cggcggtcga ggcggcgctg gatatcctcg acaagggcgg caacgcggtt
     2041 gacgcagccg caacggccgc ctttgccgtt ggtgttgtcg agccgtggat gagcggcatc
     2101 ggcggcgtcg gcttcatgac gatccagatg gccggcaagg agccggtcgt aatcgactac
     2161 ttcggccgcg cgccaggggc ggcgacgccc gatatgtacg agctgaccgg cgaggagcgc
     2221 tcggtcgtcg gctttggcgg cgtcaaggat caggccaacg cctacggcgc gctctcctgc
     2281 accgtgccgg ggatggtggc cggcctcgcc tacgccgtcg agcactacgg caagaaggag
     2341 ctggccgaga tcgtcgcccc ggccgcccgg ctggccgacg agggcttcga ggtcaactgg
     2401 tacaacggga tgctgctctc ctcgcagcag aagacgcttc agcgcgacgc tgagtccgcc
     2461 cgcatcttcc tcaacaacgg cacaccgccg gcgccgctct tcggacagcc gaacccgcgc
     2521 atccgccagc cggagctggc cgggacgctg cgcaagatcg cggcgcaggg cagggacggc
     2581 ttctacaagg gcgaggtggc cgagaagatc gccagccacc ttcagagcct cggcggcatc
     2641 atgacggccg acgacctggc ccgctacgag ccggtcgtca acaagccgat cgtcatcccc
     2701 taccagggtt acgaactggt cctgctgccg ttccagggcg gcggcatcac gctggccgag
     2761 accttcaaca tcctcgacgg tctcaatatc cgcgcgaccg gccacaacac ggcccagacg
     2821 ctgcacagca tcgaccaggc cagcaagagc gccttcgccg accgcttcgc cttcatcggc
     2881 gacccggatt atgtcgatat tgattgggac aagctggcct cgaaggccta cgccgaccag
     2941 cgccgggccg cgctcgaccc gaatcgcgcg accgtgccgc agccgggcga cggtatccgc
     3001 cgtggcagcg gcctcaagcc ggagatggcc gcccagatgg acggcggctg cacgacgcat
     3061 ctctcggtcg tcgacaagga cggcaacatg gtctccgtca cccagacgct gacgctgatc
     3121 ttcggctcgg tcgtcacgat ccccggtgtc ggtgtcatca tgaacgacac gatgaacctg
     3181 ttcgagccga tccctggccg ggctaactcg atcgcctcct ggaagcggcc ggcctccaac
     3241 atggcccatg tcgtcgcgac gaaggacggc gtcccggtcc tggccgtcgg cgcgccgggc
     3301 ggccgccgga tcatcgacac ctgcctgcag atgacgctga atgtcctcga cttcgacatg
     3361 gacatcgcgg cagcctgcgg cgcgccactg atcgactgct ccggcgccga gctgctggcc
     3421 gacgaccgca tcccgcaagc aacccgcgac cggctgcggg cgatgggcca cgatgtcgtc
     3481 gacgccgagg tctcgttctc cccgcgcgcc ttcgccagcc cgaccggtgt caccgtcgac
     3541 cccgagaccg gcctgcgcgg cggcggcgcc gacccgttcg gcattggcat cgcggccggc
     3601 cggtagcacg agaaaccatc cgggcttcgt gacgggaata cacccgtcgc gaagcccttc
     3661 cccttctcta tttcccgcgc aatctggcga cgaccggcgc aaagaattcc atgtcctcgg
     3721 gaaagacgct gaagtgggaa atgccaaacc gctcacgcag gccaaggatc tggtcgacga
     3781 tcgcgtcaac gctaccgagc aggtagtacg gcgacgccag cacttcatcg gcggtcacgc
     3841 cacgggcacc gctggcgatc gtttccgctg cggctcggcg gttgtcggtg atgatgacgt
     3901 cccagatgag catggcaagc tcgagccgct cgaaacgggc tccggcagcc gaccgtaccc
     3961 agtcgacctt ccgggcgatc gcgtcatcgg tctcatcatt gccgaatccc agtccgccag
     4021 ccggccgcgc ccgggccagg ataccgacgg tgtcggcctc ctgtgccgcg aacgtcagca
     4081 atcgcttccc gccgccaccg atgaagacgg gcgggtgcgg ttgctggacc ggacggacac
     4141 agtcggtcag gccggtgatc gtgtagtgtg ctccacaatg atgaacttct tcgccggacc
     4201 atagccgctt gatgatcgag accgcctcga tcatacgatc tacgcgggtg ccgggagcgt
     4261 cgaacgggat acctgtctgc tcatactccc gcttgaccca gccggcgccg atcccaaact
     4321 cgaagcggcc atcgctgagc atatccagcg ttgccgtctc gttggccaga atagccggat
     4381 gacgaaagtc gttgtcgaag acggtgcagc caatccgcag cgacgtcgtc gcgtaggccg
     4441 ccgcgagcag acccaccgcc ggggcaaggt tctgtccgaa gtggtcaggc atcaggaacg
     4501 cgtgatagcc aagcgcctcg acccggcgcg cgaattcgga ccacgcctgg ctggacgaag
     4561 cggtaaatgc cccacaggca aaccgaaatg gacgttgcct cgtcatagca gcaacccctc
     4621 tcagattcag ttgtgcgcct cacgcgatcg atgcgcccag catacgctcc atgagcacag
     4681 ccgtataggg ggaagctcgt caccgccttt cggcaacaat gccggtgcgc attcagtgtc
     4741 atcgcccatg cgatactgcg gaccagaacc atatctacgg gaagaggaga tggccgtgtc
     4801 cggacaacca accgagtatc tgtcgatgcg acggatcggt gacgccgaga tgacgttgat
     4861 actggagggg gccgggccgt atccggtcga gctcaatgtg ccggaagatg tctggcggcc
     4921 ggccgtgccg gacgccgatg cgaacgggaa cgtgctgctc tgcagtggcg gcgcgtacgt
     4981 ccgaatcgga gacgcctcga tcgtcatcga tccggggctg gacgatcccg gcacgccaac
     5041 ctcagccgcc accgagctgg tcttcaagga ctggtcattc acgccggggt tccaggcggc
     5101 gctggatgag ttgggtgtcg acaacgatgc cgtcacgcac gtcatcatta cccacgccca
     5161 cttcgaccac tgcctcggcg tcgccgtcga gcgggatggc aagctcgtgc cgcgctaccc
     5221 gaacgcccgc tacctgctcg accgggccga ctgggatctg catttcacac ctgacggcga
     5281 gc
//
LOCUS       DASUGJ010000107         4131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1543270, whole genome shotgun sequence.
ACCESSION   DASUGJ010000107 DASUGJ010000000
VERSION     DASUGJ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543270"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1046)
                     /locus_tag="VFV93_02375"
     CDS             complement(93..1046)
                     /locus_tag="VFV93_02375"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX5164217.1"
                     /translation="MAAGKPTYLWWNGKRMNWDDATVHVTALGWSTVGAIFEGIRAYW
                     NPDHEELYVFRLDEHMDRFARSLKLVRLPETWSMDELKAAIIDLLQANETREDTYIRP
                     LAYRGGEGRGGFSGIGGETEILINTHPNPSHLMSGKAQHANVASWRRISENVMPPRIK
                     NISNYRNSQLANMESRDGGYDIPVILNEQGKVAEGPGACIALIRDGKFVTPDTTQGIL
                     ESITRDALIELARTELGMVVEERAVDRTELYIADEVFMCGTAAEISPIISVDRYEVGN
                     GQIGPVTQQLEALLDDCFRGKTGVRPEWRTPVGVAKPVAAD"
     gene            complement(1284..3188)
                     /locus_tag="VFV93_02380"
     CDS             complement(1284..3188)
                     /locus_tag="VFV93_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5164218.1"
                     /translation="MIPIDKEQVRLLEQRLRGIGVDRRTFLKIAGAAMAAPAAGSLLA
                     ACGGDDDDDDDGGEPTNTTAAAAPTNTTAASGGGEATATTAEQAAATPTTGGGTTASP
                     TTAAPEATATEAMEIDADQTLHTIGTRSEPASHDFNADLYCGGVAQIWMGLSTYDVNL
                     EPAPGWATEWEPNEDASMWTYHIRPDNTGFSNGDPVTATTFAESWRRLLLPETKAPYA
                     SILFDIKGAQEINLEGADPSTLGVRAVDDWTLEVDMVGPRGVFPVIAGYVACVPTHIK
                     SVEAGNYSTDPADGEVISNGPFKLVEWNHNEDCQLEKNENYWDAENIILTNIDWKIVP
                     AEQGMLPYEAGEVDWALVPGADLPRVQSDATMSQELEKWVEPLIWKILPGTNVKPFDD
                     INARHALQHSIDRERINELTNGGGDPAYCLMPPGLFAYFGEEFKEDCAFDLDKANEFL
                     AQSAYADKNWPKVTMIERNEAQLNSTIMSEDVAAQVLENLGLEIELQIMDLQAFRDLQ
                     FQNTYELCWIRWFYDYPDPNNGYFDMFYSNKDSGKRQAWSNPEFDQLTLDGKAEPDPE
                     KRLDIYRQCEQIMHDDVAYIPIVYRNAYYVYKPWMKGVPVANTGFVTPNGNIYLGMYD
                     RVYIQGREDA"
BASE COUNT          703 a         1258 c         1323 g          847 t
ORIGIN      
        1 ccggtgttgt gccggccctg ctcgtgtccc cgaacgcgtt gaggggagaa gggccggcac
       61 aagaccggcc cctacttcct ggcgatggtt ggttagtcgg cagcgaccgg cttggcgacg
      121 ccgaccgggg tgcgccactc ggggcgaacg ccggtcttgc cgcggaagca gtcatcgagc
      181 agcgcttcga gctgctgggt gacggggccg atctgaccgt tgccgacctc gtagcggtcg
      241 accgagatga tcggcgagat ctccgcggcc gtgccgcaca tgaagacctc gtcggcgatg
      301 tagagctcgg tccggtcgac agcgcgctct tcgacaacca tgcccagctc ggtgcgggcg
      361 agttcgatca gcgcgtcgcg agtgatgctc tccaggatgc cctgggtcgt gtccggcgtg
      421 acgaacttgc cgtcgcggat cagcgcgatg caggcgcccg gaccctcggc gaccttgccc
      481 tgctcgttga ggatgaccgg gatatcgtag ccgccgtcgc gcgactccat gttggcaagc
      541 tggctgttgc gatagttgga gatgttcttg atgcgcggcg gcatcacgtt ctccgagatg
      601 cgccgccatg acgcgacgtt ggcgtgctgg gccttgccgc tcatcaggtg ggacgggttc
      661 ggatgcgtgt tgatgaggat ctctgtttcg ccgccgatgc cgctgaagcc gccccggcct
      721 tcgccgcctc ggtaggccag cggccggatg taggtatctt cgcgggtttc gttggcctgg
      781 agcaggtcga tgatggcggc cttgagctcg tccatcgacc aggtttccgg cagccggacc
      841 agcttgagcg agcgcgcgaa gcggtccatg tgctcgtcga gtcggaagac gtacagctcc
      901 tcgtgatcgg gattccagta ggcgcggatg ccctcgaaga tcgcgccgac cgtcgaccag
      961 cccagcgcgg tgacgtggac ggtcgcgtcg tcccagttca tcctcttgcc gttccaccag
     1021 agatacgttg gtttgcctgc tgccaccgtt gtgctctcct cactcagcta tcatgccggg
     1081 ccgcatagga tccccggcga aatcagaata gtggaccagt ttgaaggata gcagagtcac
     1141 cgatgccgca aagtgccaaa tcggatcacg ccagaccatc caatcgctcg gctggaccgg
     1201 ccttgcgagc gccaccggac acggagcggg gggagccaat cggctccccc cgcttgctgt
     1261 ctgatccgat cgatgtgact cgatcaggcg tcttcgcgac cctggatgta gacgcggtcg
     1321 tacatgccaa ggtagatgtt gccgtttggc gtcacgaagc ccgtgttggc aaccggaacg
     1381 cccttcatcc acggcttgta gacatagtag gcgttgcggt acacgatcgg gatataggcg
     1441 acgtcgtcgt gcatgatctg ctcgcactgg cggtagatgt cgagccgctt ctcgggatcc
     1501 ggctcggcct tcccgtcgag ggtgagctgg tcgaactccg gattggacca tgcctggcgc
     1561 ttgccggagt ccttgttcga gtagaacatg tcgaagtagc cgttgttcgg atccgggtag
     1621 tcgtagaacc agcggatcca gcacagttcg taggtattct ggaactgcag gtcgcggaac
     1681 gcctgcaggt ccatgatctg cagctcgatc tcaagtccga ggttctcgag cacctgggcg
     1741 gcaacgtcct ccgacatgat cgtcgagttc agctgggcct cgttgcgctc gatcatcgtg
     1801 accttcggcc agttcttgtc ggcgtaggcc gactgggcca ggaactcatt ggccttgtca
     1861 agatcgaagg cgcagtcttc cttgaactct tcaccgaagt aggcgaacag gcccggcggc
     1921 atcaggcagt aggccggatc gccaccaccg ttggtcagct cgttgatgcg ctcgcggtcg
     1981 atggagtgct ggagggcgtg gcgggcattg atgtcgtcga acggcttgac gttcgtgccc
     2041 ggcaggatct tccagatcag cggctcgacc cacttctcga gctcctggct catggtggca
     2101 tccgactgaa cacgcggcag gtcggcgccg gggacaagcg cccagtcaac ctcacccgcc
     2161 tcgtacggga gcatgccctg ctcggccggg acgatcttcc agtcgatgtt ggtgaggatg
     2221 atgttctcgg cgtcccagta gttctcgttc ttctcgagct ggcagtcctc gttgtgattc
     2281 cactcgacca gcttgaacgg gccgtttgag atcacctcgc cgtctgccgg gtcggtcgag
     2341 tagttgccgg cctcgactga cttgatatgg gtcgggacgc aggcgacgta gccggcgatg
     2401 accgggaaga caccgcgcgg gccgaccatg tcaacttcga gcgtccaatc atcaacagcg
     2461 cgaacgccga gcgtagacgg atcggcacct tcgaggttga tctcctgagc gcccttgatg
     2521 tcgaacagga tcgaagcgta aggggccttc gtttccggca gcaacaggcg ccgccacgat
     2581 tccgcgaagg ttgtcgccgt gaccgggtcg ccgttcgaga aaccggtgtt gtcagggcgg
     2641 atgtggtagg tccacatcga cgcgtcctcg ttcggctccc actcggtcgc ccaacctgga
     2701 gccggctcca ggttgacgtc gtaggtcgag aggcccatcc agatctgggc aacgccgccg
     2761 caatacagat cggcgttgaa gtcgtggctg gctggttcgg agcgggtgcc gattgtatgc
     2821 agtgtctggt cggcgtcgat ctccatcgcc tcggttgcgg tcgcctcagg agccgccgtc
     2881 gttgggctcg ctgtcgttcc gccacctgta gtcggagttg ccgcggcttg ctcggccgtc
     2941 gtcgcggttg cttcgccgcc gccggaggct gcggtggtgt tggttggggc tgccgctgcg
     3001 gtggtgttgg ttggctcgcc gccatcatcg tcatcgtcgt cgtcaccgcc gcaggcagcc
     3061 agcagcgagc ctgccgccgg agccgccatc gccgcgccgg cgatcttcag gaaggtgcgg
     3121 cggtcaacgc caattccacg caggcgttgc tcgagtagtc ggacctgttc tttgtcgatc
     3181 ggaatcatcg agagaactcc tcctcgtatc ggtaaccaac atggctgcct tccaccccgt
     3241 atcacctaac tgaccacggt agccgtggcc ggcgaagaat tcaggcagcg tgacggcaca
     3301 acaacgcagc agagcctgga aagctcctct acggcaatgg atcctgggcg gcccctcctc
     3361 tcgctggacg catggatcgt agcagtgctg tctggtggtg ctacccctgt atacggatgg
     3421 cggtgcgatt ctggtcccat ccagactggc caacatccac actacgacgc gaactcacgt
     3481 tggaagacgg cgcctgctca accccatgcg cctccccatt ggcgcgactg gtgggagtcg
     3541 gtcccttcgg tgaaccaggt acgcacaagc cacctgtgcc gaagcgcgca tgtcttcccg
     3601 tgcgctgtcc gcatgcccgt gtgtgaatga cggtacctat gtagcacagc gaatcaggaa
     3661 tggcaagtca tccgcgaagg aagtgcgttg caggcgaatt gttatcgttc gcagcgtgtt
     3721 cagaccggat agtgtgcata tgcgcataat agaatgcagc ggcttgcggt ggccatcggg
     3781 aggttccgat ccgcaatctc tcaatccccc caatccggta cgggcgatat cgcaatatcg
     3841 ccctgcaacc ccaccgaggc gttcgggcgg gacacgaagg gcgatattgc gatatcgccc
     3901 gtaccggatt gaggcattgg agattggcca gcattggaca cggaacgact tcgatgaccg
     3961 ccgcaagtgc ttgaattctc gtatgcgcat atgctcacta ttcggtctga acgcgctgcg
     4021 aacgataaca gttcgcctgc aacgcacttc cttcgcggat gacttgccat tcctgattcg
     4081 ctgtgctaca taggtaccgt cattcacaca cgggcatgcg gacagcgcac g
//
LOCUS       DASUGJ010000108         4397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1125534, whole genome shotgun sequence.
ACCESSION   DASUGJ010000108 DASUGJ010000000
VERSION     DASUGJ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125534"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VFV93_02385"
     CDS             complement(<1..1184)
                     /locus_tag="VFV93_02385"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX5164219.1"
                     /translation="MPKYIFVTGGVVSSVGKGIATASIGRLLKARGLHVSIMKLDPYL
                     NVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFTDENVNRGSNVTTGQVYSSVIAKE
                     RRGDYLGGTIQVIPHITNEIKDRIHQVATARDVDAVIVEVGGTVGDIESQPFLEAIRQ
                     LRKDEGRRNVLYIHVTLLPQIGSTGEMKTKPTQHSVRDLRATGIQPDVIICRSDYDVL
                     PEVISKIALFTDVDEHAVIPLKTAETIYEVPLMLEEAGLGDYLAQHFSWPATGELRAW
                     RGMVERIKTPRRGLEIALVGKYVDLHDSYMSISEALTHAGLAHDVDVNINWVNSERVT
                     LAELEEQLAHACGILVAPGFGPRGVEGKILASRYARERQIPYLGVCYGLHMAAIDVAR
                     NVL"
     gene            complement(1303..1938)
                     /locus_tag="VFV93_02390"
     CDS             complement(1303..1938)
                     /locus_tag="VFV93_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5164220.1"
                     /translation="MAEIKWFGHACFRLRSRDAMILTDPVPRSFGYKIDKQKVDIITI
                     SHDHPGHTNVDLASTSPKLVNGPGEYEMSDVFITGIRTYHDDKQGAEHGRNTAYLFDL
                     EDLVVCHLGDLGHTLSEGQVETMSIADVVIIPVGGGTVLNAEKAVEVIGQLEPRVIIP
                     MQYQTPMGDKQRDPLERFLKEMGVTEVTRRDKLTVRASDLGDTPEVVVLEP"
     gene            complement(2074..2508)
                     /gene="mce"
                     /locus_tag="VFV93_02395"
     CDS             complement(2074..2508)
                     /gene="mce"
                     /locus_tag="VFV93_02395"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX5164221.1"
                     /translation="MPGSRFTPPGPIHHIGVVVEDIAAAVEQYRLLGFGEGDITRVSE
                     QNVDIAAMRAGESWVEILSPIDRDSGIGRFLASRGPGFHHIAYLVDDLAGTLAALAEA
                     GVELIDQSPRVGLHDWRVAFVHPRACAGVLSELVERSSVGEP"
     gene            complement(2577..>4397)
                     /locus_tag="VFV93_02400"
     CDS             complement(2577..>4397)
                     /locus_tag="VFV93_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5164222.1"
                     /translation="DGPTLLAKITSQGAYLESLRDNLSDAEAEELKASLSDIPGIRVL
                     SELNFALETHASPDLPMVVKQDISREKALELAANVVYLPGVIVDDTTLIRQYAGGPAF
                     SHLLGYVGPISEQEYDAETTPTGGHIYQPDDNVGRGGVEEALEKRMRGEKGIRWIQVD
                     SAGVERLELVDLRRDPTDGLSARLTVVQQFQLAVTQALQEGIEFANSEALKAHPPKPE
                     DANKPVVGAGVAIVINPRNGEVLAMVSLPTFDNQKFIGGISQEDYNAYLNDEFTPLLN
                     RAISGEYPPGSVFKPLLACSALQSKVITPQDTFKCLGSIRVPWTWDETQGNTYPCYDP
                     AGHGDVDLFKGISQSCDVYFYNVGAPKSQTDAGVNVHYYIPNDPNPHEFRGMGIETIE
                     KYIKDVFGFGTPTGIELAGEAEGLVPNPKWLFQSDLHEYWSIGDTINVSIGQGHLLCT
                     PLQLLNGTAAIANGGNLYVPRLIRDLVDESGTVVERFPPKRLRELQIDKQHINDVREG
                     MRRTVTEGTAMGKITINDVEVGAKSGTAEFGEADTEGEYKNSHAWFTAFAPYNDPEIC
                     LAVMIQGGGAGSVYAGPVANKILDAYFHTPGIREAAQSTS"
BASE COUNT          747 a         1400 c         1424 g          826 t
ORIGIN      
        1 agcacgttgc gggcgacgtc gatcgcggcc atgtgcaggc cgtagcagac gcccagatac
       61 ggaatctgcc gctcgcgggc atagcgcgac gccaggatct tgccctcgac accgcgcggc
      121 ccgaagcccg gcgcgaccag gatgccgcag gcgtgcgcca gctgctcttc caactcggcc
      181 agcgtgacgc gctcggagtt gacccagttg atgttgacat cgacatcatg cgccagcccg
      241 gcgtgggtca gcgcctcgga gatgctcatg tacgagtcgt gcagatcgac gtacttgcca
      301 accagcgcga tctccagccc gcgacgcggg gtcttgatgc gctcgaccat cccgcgccag
      361 gcacgcaatt cgccggtggc cggccaggag aagtgctgag cgaggtagtc accgaggccg
      421 gcctcctcca gcatcaacgg cacctcgtag atcgtctcgg ccgtcttgag tgggatgacg
      481 gcatgctcgt cgacatcggt gaagagcgcg atcttggaga taacctccgg cagcacatcg
      541 tagtccgagc ggcagatgat cacatccggc tggataccgg tcgcccgcag gtcgcggacg
      601 gagtgctggg tcggcttggt cttcatctca ccggtcgagc cgatctgcgg caggagcgtg
      661 acgtggatgt agaggacgtt gcggcggccc tcatccttgc gcaactggcg gatcgcctcc
      721 aggaacggct gcgactcgat gtcgccgacc gtcccgccga cctcgacgat gacggcgtcg
      781 acgtcacggg ccgttgcgac ctggtggatg cggtccttga tctcgttggt gatgtgcggg
      841 atgacctgga tcgtgccacc gaggtagtcg ccacggcgct ccttggcgat gacggatgag
      901 tagacctggc cggtcgtgac gttcgagcca cggttgacat tctcgtccgt gaagcgctcg
      961 tagtggccca ggtcgaggtc ggtttcggcg ccgtcctcgg tcacgaacac ctcaccgtgc
     1021 tggtagggcg acatcgtgcc agggtcaacg ttcagatacg ggtcgagctt catgatcgag
     1081 acgtgcaggc cgcgcgcctt gagcaaccgc ccgatcgagg cagttgcgat tcccttgcca
     1141 accgacgaga cgacaccacc cgtcacgaag atgtatttcg gcacccttca gctcctcggt
     1201 cagctaccag ccattagcaa tcagctacca gccatcggcg atccgactgc acgtcgtttg
     1261 cctcggtgag gcggacggca gccggcagat ggctgatagc tgctacggtt cgagtaccac
     1321 cacctccggc gtgtcgccga gatcgctggc gcgaacggtc agcttgtcgc gccgggtcac
     1381 ctcggtgacg cccatctcct tcaggaatcg ctccaacgga tcacgctgct tgtcgcccat
     1441 cggcgtctga tactgcatcg ggatgatgac gcgcggctcg agctgaccga tcacctcaac
     1501 cgccttctcg gcgttgagaa cagtgccgcc gccaaccggg atgatgacga catcggcgat
     1561 gctcatcgtc tcgacctggc cctcgctcag cgtgtgaccg aggtcgccca ggtggcagac
     1621 gacgaggtct tcgaggtcga acagataggc ggtgttgcgc ccatgctcgg ccccctgctt
     1681 gtcatcgtgg taggtgcgga tgccggtgat gaagacgtcg ctcatctcgt actcgccggg
     1741 gccgttgacc agcttcggcg acgtcgatgc cagatcgacg ttggtgtgtc cggggtgatc
     1801 gtgggagatg gtgatgatat cgactttttg cttgtcgatc ttgtagccga acgagcgcgg
     1861 cacgggatcg gtcagaatca tggcatcacg cgaacgcaaa cggaagcacg cgtggccaaa
     1921 ccacttgatc tcagccatgg ttgccttacg ttccagaacg atgttgagat gtacaggtgg
     1981 gctcttccgg aggtttctca gggtgcctag acgatcatat cacgcactga acggacacgc
     2041 taccattttt ggaacgtctg ttcggaacgc ggctcaaggc tcgccgacgc tggagcgctc
     2101 gaccaactcg ctgagcaccc cggcgcaggc gcgcgggtgg acgaacgcga cccgccagtc
     2161 gtgcaacccg acgcgtggcg attggtcgat cagctcgacg ccggcctcgg ccagcgcagc
     2221 cagcgtcccg gccagatcgt caaccagata ggcgatgtgg tggaagcccg gcccgcgtga
     2281 ggcgagaaag cggccgatcc ccgaatcgcg gtcgatcggc gacaggatct cgacccacga
     2341 ctcccctgcc cgcatcgccg cgatatcgac gttctgctcg gacacgcgcg ttatgtcgcc
     2401 ttcaccgaag ccgagcagcc ggtactgctc gacagccgcg gcgatgtcct caacgacgac
     2461 gccgatgtgg tggatcggtc cgggaggggt gaatcttgaa ccaggcacgt accattctca
     2521 catttcgtag ggacaggtct gagacccgtc cttacgggtg tgcgagcgtg tagcaatcac
     2581 gacgttgact gcgccgcctc gcggatgccg ggtgtatgga agtaggcgtc gaggatcttg
     2641 ttggcgaccg ggccggcgta gacgctaccg gcgccgccgc cctggatcat gacggccagg
     2701 cagatctccg ggtcgttgta gggcgcgaag gccgtgaacc aggcgtgcga gttcttgtac
     2761 tcaccctcgg tgtccgcttc gccaaactcg gccgtgccgc tcttggcgcc gacctcgaca
     2821 tcgttgatgg tgatcttgcc catcgccgtg ccctcggtga cggtccgacg catcccctcg
     2881 cgaacgtcgt tgatgtgctg cttgtcgatc tgtagctcgc gcagtcgctt gggcgggaag
     2941 cgctcgacga ccgtgccgga ctcatcgacc agatcgcgga tcaggcgtgg cacgtagaga
     3001 ttgccgccgt tcgcaatcgc ggccgtcccg ttgaggagct ggagcggcgt gcacagcagg
     3061 tggccctgac cgatcgagac gttgatcgtg tcgccgatcg accagtactc gtgcaggtcg
     3121 gactggaaca gccacttcgg attcggcacc agcccctcgg cctcgccggc cagctcgatg
     3181 ccggtcggcg tgccgaagcc gaagacgtcc ttgatgtact tctcgatcgt ctcgatcccc
     3241 atcccacgaa actcgtgcgg gttcgggtcg ttcgggatgt agtagtgcac attcacgccg
     3301 gcgtcggtct gcgacttcgg cgcgccgacg ttgtagaagt agacatcgca cgactgcgag
     3361 atgcctttga agagatcgac atcgccgtga ccggccgggt catagcaggg gtaggtattg
     3421 ccctgcgtct cgtcccaggt ccacggcacg cggatcgacc cgaggcactt gaacgtgtcc
     3481 tgcggggtga tgaccttcga ttgcagcgcc gagcaggcca gcagcggctt gaagacggag
     3541 ccgggcgggt actcgccgct gatggcgcgg ttgagcagcg gcgtgaactc gtcgttcagg
     3601 taggcgttgt aatcctcctg cgagatgccg ccgatgaact tctggttgtc gaaggtcggc
     3661 aacgagacca tcgccaggac ctcgccgttg cgcggattga tgacgatcgc gaccccggcg
     3721 ccgacaacgg gcttgttcgc gtcctccggc ttcggcggat gcgccttgag tgcctcgctg
     3781 ttggcgaact cgatcccctc ctgcagcgcc tgggtaacgg ccagctggaa ctgctggacg
     3841 accgtcagcc gggccgacag gccgtcggtc ggatcacggc gcaggtcgac cagctccagc
     3901 cgctcgacgc cggccgaatc gacctgaatc cagcggatgc ccttctcgcc gcgcatccgc
     3961 ttctcgagcg cttcctcgac gccaccacgc ccgacattgt cgtccggctg gtagatgtgg
     4021 ccgccggtcg gcgtcgtctc ggcgtcgtac tcctgctcgc tgatcgggcc gacgtagccg
     4081 agcaggtggg agaaggccgg cccacccgca tattggcgga tcagggtcgt gtcgtcgacg
     4141 atcacacccg gcaggtagac gacgttggcg gccagctcca gtgccttctc ccggctgatg
     4201 tcctgcttga caaccatcgg cagatcgggc gaagcgtgcg tctcgagcgc gaaattcagc
     4261 tcgctcaaca ccctgatgcc tggaatgtcg gacaggctgg ccttcaactc ctccgcctcg
     4321 gcgtcgctga ggttgtcccg cagcgattcg agataggcgc cctgactggt gatcttcgcc
     4381 agcaaggtcg ggccatc
//
LOCUS       DASUGJ010000109         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_570217, whole genome shotgun sequence.
ACCESSION   DASUGJ010000109 DASUGJ010000000
VERSION     DASUGJ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570217"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1530
                     /locus_tag="VFV93_02405"
     CDS             28..1530
                     /locus_tag="VFV93_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164223.1"
                     /translation="MCDTFDWVKPSPLWQLEAQDFRQAGFFRPQLLEFRSDAFMEDFF
                     AVAGDPKPAAIGAVVTPRPGETAALKLYQPVHGRFYLACASLCCRVPGFPDREVRTAD
                     GESTFFVLRKLLGGEEFGWVVSGTQKGWKPLNGQPRRVLANEERLPLLPAQAGNCRSL
                     LFGYIPVASRETYAVPAATLETDTGGGTTAAPDTRVEEFGSRFNGPLDALDDIPSSVS
                     ASIVLSMSVAMLLDLYEFLDRNLPDVAAALASGAGTDGPFTDKADERAALITALDEQE
                     MGTGVTLASALAKAAANQEALNAPGGGDLGGLGFTSAYSLQGRSIEQGRLSDAVRAAL
                     PDALPDVELPKLESGADVQYTLRCVYERPQCDPPVVVVSQPSLPFQLAPFFDADAPAR
                     TVRIPLPADVSVAGLRKFKQGVTFIMSDAMRKKVSMVTGHEKDLLEDPPSVGPEDGGA
                     FAFVCSFSIQIIFIVAFMLLLIFVFVLNIIFSWIIFFRICLPIPKSWAPK"
     gene            1527..1925
                     /locus_tag="VFV93_02410"
     CDS             1527..1925
                     /locus_tag="VFV93_02410"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX5164224.1"
                     /translation="MSIRKVDIYGQGLSFPPRVGADGRLAWSGDEENVRESIRVILLT
                     EPGERLMRPGFGCGLRRFLFEPNTPTTRALIRERIVRAISDWEPRVRLDAVEVEPDPN
                     EPRTVQVQISFRLVATNAMGRLGMSLQLEG"
     gene            1929..>2245
                     /locus_tag="VFV93_02415"
     CDS             1929..>2245
                     /locus_tag="VFV93_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164225.1"
                     /translation="MPLQAPLLDTTSFEQLLAEARRRIPGHTPEWTNFAGESDPGITI
                     VELFAFLTDSLLYRANRVPERNYIKYLQLLGIPLQPAAAARGIATIGNERGPAAPLPL
                     DRG"
BASE COUNT          343 a          750 c          772 g          380 t
ORIGIN      
        1 tggtagggca ggagagcgag ccgttggatg tgtgacacgt tcgattgggt caagccatcg
       61 ccactctggc aactggaggc gcaggacttc cgccaggccg gcttcttccg gccgcagttg
      121 ctggagttcc gctccgacgc gttcatggag gacttcttcg ccgtcgccgg cgacccgaag
      181 ccggcggcga tcggggctgt cgtcacgccg cggccgggcg agacggcagc gctcaagctc
      241 taccagccgg tccacgggcg tttctacctg gcctgcgcat cgctctgctg ccgggtgccg
      301 ggcttcccgg accgcgaagt acgaacggcc gacggtgaga gcaccttctt cgtcctgcgc
      361 aagctgctgg gcggcgagga gttcggctgg gtcgtctcgg gaacgcagaa gggctggaag
      421 ccgctgaacg gccagccgcg gagggtgctg gcgaacgagg agcggctgcc gctgctcccc
      481 gcgcaagccg gcaactgccg ctcgctgctg tttggctaca tcccggtcgc cagccgcgag
      541 acgtacgccg tcccggccgc gacgctggag acggacacgg gcggtggcac gacggccgcg
      601 ccggacaccc gcgtcgagga gttcggttcg cgcttcaatg gcccgctcga cgcgctggac
      661 gacattccaa gtagcgtcag cgcaagcatc gtcctctcga tgtcggtcgc gatgctgctc
      721 gatctctacg agttcctcga ccgcaacctg ccggatgtcg ccgctgcgct tgcgtctggc
      781 gcgggaaccg atggcccgtt caccgacaag gccgacgaac gggcggcgct catcacggcg
      841 cttgacgaac aggagatggg gaccggcgtg acattggcgt cggcgctggc gaaggcggcg
      901 gccaaccagg aagcgctcaa cgcgcccggt ggcggtgacc ttggcgggct gggcttcacg
      961 agcgcctaca gcctgcaggg ccgcagcatc gagcagggcc ggctcagcga cgcggtccgg
     1021 gcggcgctgc cggatgcgct gccggatgtc gagctgccga agctcgaatc gggagccgac
     1081 gtccagtaca cgctgcgctg cgtctacgag cgtccgcagt gcgacccgcc ggtggttgtc
     1141 gtcagccagc cgtcgctgcc gttccagctg gcgccgttct tcgacgccga cgcgccggcc
     1201 cggacggtcc ggattccgtt gccggccgat gtcagcgtgg ccgggctgcg caagttcaag
     1261 cagggcgtca ccttcatcat gtccgacgcg atgcgcaaga aggtcagcat ggtgacgggg
     1321 catgagaagg acctgctcga agacccgccc tcggtcgggc cggaggatgg tggcgcgttc
     1381 gccttcgtct gctcgttctc gatccagatc atcttcatcg tcgcctttat gctgctgctg
     1441 atctttgttt tcgtattgaa catcattttc tcgtggatca tctttttccg gatctgtctg
     1501 ccgataccaa agagctgggc gccaaaatga gcatacgcaa agtagacatc tacggccagg
     1561 gcctcagttt cccgccgcgc gtcggcgccg atggccggct ggcgtggtcg ggcgacgagg
     1621 agaacgtgcg cgagtccatc cgcgtgatcc tgctcaccga gccgggcgag cgcctgatgc
     1681 ggccgggctt tggctgcggg ctgcgccgct tcctgttcga gccgaacacg ccgaccacgc
     1741 gcgcgctgat ccgcgagcgg atcgtgcgcg cgatcagcga ctgggagccg cgcgtgcgcc
     1801 tggatgcggt cgaggtcgag cccgacccga acgagccgcg cactgtgcag gtgcagatca
     1861 gctttcgcct tgtggctacg aacgcgatgg gccggctcgg gatgagtttg cagctggagg
     1921 gttagccgat gccgctacaa gcaccgcttc tcgatacgac cagcttcgag cagctgctcg
     1981 ccgaggccag gcgccgcatc ccggggcata cgccggagtg gaccaacttc gccggcgaga
     2041 gcgaccccgg gatcacgatc gtcgagctgt ttgcgttcct gaccgacagc ctgctctacc
     2101 gcgccaaccg cgtgcccgag cgcaactata tcaagtattt gcaactgctc ggcatcccgc
     2161 tccagcccgc cgccgccgcc cgcggcatcg cgacgatcgg caacgagcgc ggcccggccg
     2221 cgccgctgcc gctcgaccgc gggat
//
LOCUS       DASUGJ010000110         6369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1543519, whole genome shotgun sequence.
ACCESSION   DASUGJ010000110 DASUGJ010000000
VERSION     DASUGJ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543519"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFV93_02420"
     CDS             complement(<1..188)
                     /locus_tag="VFV93_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164226.1"
                     /translation="MAATKPEGQAVAALVFDALKADTGAGGVNTLVGGRIYRERAPQA
                     AAVPAVVIGGVTWEPANTV"
     gene            complement(188..502)
                     /locus_tag="VFV93_02425"
     CDS             complement(188..502)
                     /locus_tag="VFV93_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HEX5164227.1"
                     /translation="MVSNRLPQVAAKVRPLVSAEVKKAGFDVQAKAQALVPVKTGLLR
                     RSITTQFPTDLSAVVGPSANYGIYVEFGTRHMGARPYMRPAAEAVLPGFAAKVAAALK
                     GL"
     gene            complement(526..873)
                     /locus_tag="VFV93_02430"
     CDS             complement(526..873)
                     /locus_tag="VFV93_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164228.1"
                     /translation="MRYLAILAALLALTIVSPVYADEFIWDAADDAGPPKGVVGYDGI
                     QCVGEATQDGHHVKLTNYVGYSAGQPEARERAWQLSECRNMMRDHMRFVFLMQWGVAF
                     RDSTPIAITTRRL"
     gene            complement(884..1021)
                     /locus_tag="VFV93_02435"
     CDS             complement(884..1021)
                     /locus_tag="VFV93_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164229.1"
                     /translation="MTLGLSGVCYLVAVILFVLAAIPFAEWNGRLSTIGLAFFALGHL
                     F"
     gene            complement(1098..1502)
                     /locus_tag="VFV93_02440"
     CDS             complement(1098..1502)
                     /locus_tag="VFV93_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164230.1"
                     /translation="MSGAASPLEISWTVIAAAGLLFSVWLMAAGWMDLEAVQQGIRAV
                     PPRARIWGPRWWVALSAVVANASLCLVWLGFILIGLIAMRFPPPPPTTEQAISNMWVG
                     WVLIGMEALLATVQGWQLFVRERVNETARGQT"
     gene            complement(1499..1663)
                     /locus_tag="VFV93_02445"
     CDS             complement(1499..1663)
                     /locus_tag="VFV93_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164231.1"
                     /translation="MQWIGTIFGWRDARPDMDDRQAFARKEAELQERLSRLEQEVEVI
                     QRAESEEPGS"
     gene            complement(1663..1860)
                     /locus_tag="VFV93_02450"
     CDS             complement(1663..1860)
                     /locus_tag="VFV93_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164232.1"
                     /translation="MDDVCLSPAALTVITAMVSGLFTALIWQMKDSIREARAQRDRAL
                     DGWEATVGLGEKAVRRERRRA"
     gene            complement(1861..2211)
                     /locus_tag="VFV93_02455"
     CDS             complement(1861..2211)
                     /locus_tag="VFV93_02455"
                     /inference="COORDINATES: protein motif:HMM:NF017343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HEX5164233.1"
                     /translation="MTIPLGSLRNLAAGFRPDTCTIQRNTQTQSGDGQADSWATVATV
                     SCRVSRIGQGGNEQLGGDASITAIGQRRIKLPAGTDVTPADRIVCNGVTYEVADVPKI
                     SNEVERTVIAREVS"
     gene            complement(2208..2411)
                     /locus_tag="VFV93_02460"
     CDS             complement(2208..2411)
                     /locus_tag="VFV93_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164234.1"
                     /translation="MPDIIIEHPKTGAQYGIDSSDYRRGKHYKDPKTGEMVTYEEAGF
                     KIVSMGDGTEYHAPAPRGEHTAP"
     gene            complement(2421..3011)
                     /locus_tag="VFV93_02465"
     CDS             complement(2421..3011)
                     /locus_tag="VFV93_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164235.1"
                     /translation="MTAYTNATNIAAYLGTTLTADQQTQAALVAQAISDWIDRETNQS
                     WQQASPATDELHTLTGNRVYLNHRPVVAVTSVKTRAAAFTAFDWTTLDASQYELLDAA
                     NGLLLIEGWSASSAALVQVSYTYTLAIPSEIALAATILASDLMTVTLHPEAAGVSQIA
                     VGQNDISVKYADASAVPPAVSRALEMVRGRRSFVIA"
     gene            complement(3008..3232)
                     /locus_tag="VFV93_02470"
     CDS             complement(3008..3232)
                     /locus_tag="VFV93_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164236.1"
                     /translation="MPLYRVGGDGIRVINPDGSGYSLPTGTVIDEIPNQYEGTLIEVE
                     GDSLLDRKPFQKRVRNYADKMIRPAEDKSL"
     gene            complement(3236..4606)
                     /locus_tag="VFV93_02475"
     CDS             complement(3236..4606)
                     /locus_tag="VFV93_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX5164237.1"
                     /translation="MSTATADNILTVEKVRNMNLSQCMAESKSRWEQAAAIEKKYPDG
                     VTPDAGEDYTQTKRLLSEIDLIETRTQELEEAGNRAQRIRDNVAKHSRPVNGHQQPQG
                     DAAEAKSNVLTMIGQQFISDPEYKRIIESGVLNNPQSRIDLGVQFKGTLLPTLARKAL
                     VYSASGQGGNLITNDRLPGYQEILQRQLTLLDLIPTTQTTSNMIEYVKEKTFTNNAAE
                     VAEATATTGTTGTKPESVLNFELATSPVQTIAHWIPVTNQMLSDAPQIRGIIDNRLVY
                     GLNQRLETEILNGDGNTPNLAGILGSGIQTLGLAAGATYGGQANVVDAAYAAMVQVQV
                     TGLANPNAFVFDPVDWAAVRLMRESAVTGNVNPGSYLYGPPSVSGPMTLWGRPVVEAI
                     GMVDNTMLVGDFQLGCMLFDREQSTIRVGLINDQFIRNMQTILAEMRAAFVVFRPTAF
                     ARVTGV"
     gene            complement(4603..5340)
                     /locus_tag="VFV93_02480"
     CDS             complement(4603..5340)
                     /locus_tag="VFV93_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEX5164238.1"
                     /translation="MIEYKSIPFEVKEVVPTEGGGWEVAGLASTWGGEPDSYGDVVVR
                     GAFAESIAKRATKFLYEHMEPIGKQIAIEENDEGLFGRWSIVDTRAGEDAYKLVKSGV
                     LDSLSIGYFTLEADYREDGVRLLRKCDLFEVSAVAIPANRNAVITDVKSLGRPFEQHS
                     EAVKATVSEWLSRVKSGSDIRATDGRALSEARKALIAAMSGSLRNAADEVEALLVPPA
                     SPERINLGMELTRRRLARHGILERPAS"
     gene            complement(5581..>6369)
                     /locus_tag="VFV93_02485"
     CDS             complement(5581..>6369)
                     /locus_tag="VFV93_02485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5164239.1"
                     /translation="THRRVELGWTEGKTFEQLEAERFERIWAMQQCDGVTVSTQRLAT
                     IVRSYTSKPVIVAPNAIDLPWFRGIVSATKRNIPGLTIGWAGGRRHDRDVEQMAEAWG
                     RIARRYPEVTFVVQGWHPPVIAQHVPDDRLVTLPWLPLESYPQGLAQIDIACASVANT
                     AFNRAKSQIKVYESAAAGAAVVASSFYAGTVENNLSGFIAETVDEWESGLAALVARPS
                     LRRLMQGRLLKTVEKRHSLAGNLWRWPSAWQQIAESARGRVFVA"
BASE COUNT          972 a         2192 c         2064 g         1141 t
ORIGIN      
        1 acggtgttgg ccggctccca ggtcacgccg ccgatcacca cggccggcac ggcagcggcc
       61 tgcggggcgc gctcccgata gatccgcccg cccacgagcg tgttgacgcc gccggccccg
      121 gtgtcggcct tcagcgcgtc gaagaccagc gcggccacgg cttgcccctc cggcttcgtg
      181 gctgccatta caagcccttc agtgcagcgg cgaccttggc cgcgaacccc ggcagcacgg
      241 cctcagccgc cggccgcatg tacgggcggg cgcccatatg ccgggtgcca aactcgacgt
      301 agatgccgta gttggccgac ggcccgacca cggctgacag gtcagtcgga aactgggtgg
      361 tgatggaccg gcgcagcagg cccgtcttga ccgggaccaa cgcctgcgcc ttggcctgca
      421 cgtcgaagcc ggccttcttg acctcggccg agacgagcgg ccggaccttc gccgccacct
      481 ggggcagtcg gttgctaacc acccggatcg tcgccttcag cacggttaga gccgcctcgt
      541 cgtgatggcg atcggcgtcg agtcccggaa cgccacgccc cactgcatca ggaagacgaa
      601 ccgcatgtgg tcgcgcatca tgttgcggca ttccgagagt tgccatgctc tctccctggc
      661 ttccggttga ccggccgaat agcccacgta gttggtcagc ttgacgtggt ggccgtcctg
      721 ggtcgcctcg ccgacgcact ggataccgtc gtagccgacg acccccttcg gcgggccggc
      781 atcgtcggcc gcgtcccaga tgaactcatc ggcgtagacc ggcgagacga tcgtcagcgc
      841 cagcagtgcg gcaaggatgg cgaggtagcg catggttagc ctcctagaag agatggccga
      901 gcgcgaagaa cgccagcccg atcgtggaca ggcgtccgtt ccattcggcg aacgggatcg
      961 cggccagcac gaacaggatg acggcgacca gatagcagac tccactcaga cccagcgtca
     1021 cggcgtcctc caggttgcgt ataggacgag cacgatcacg cagatcgcgg cagcgatggc
     1081 gaagcgctcg gcgcggctca tgtctgcccc ctcgccgtct cgttgacccg ttcccgcacg
     1141 aacagttgcc agccctggac ggtagctaac agcgcctcca tgccgatcag cacccagccg
     1201 acccacatat tcgagatcgc ctgctcggtg gtcggcggcg gcggcgggaa gcgcatcgcg
     1261 atcaggccga tcaggatgaa gccgagccag accaggcaca gcgaggcgtt cgccaccacg
     1321 gccgagagcg ccacccacca gcgcggcccc cagatacggg cgcgtggtgg tacggcccgg
     1381 attccctgtt gcacggcctc gaggtccatc cagccggcag ccatcagcca cacgctgaac
     1441 agcagcccgg cggcggcgat caccgtccag gagatttcga gcggcgacgc ggccccgctc
     1501 acgatcccgg ctcctcgctc tcggcgcgct ggatcacttc gacttcctgc tctaacctgc
     1561 tgagtcgctc ttgcagctcg gcttccttgc gagcgaatgc ctgccggtcg tccatgtccg
     1621 gccgagcatc acgccacccg aagatcgtgc cgatccactg catcacgctc tccgtcgctc
     1681 gcgccgtacg gccttctcgc ccagcccaac cgtcgcctcc cacccgtcga gcgcccggtc
     1741 tcgctgcgct ctggcctccc ggatggaatc cttcatctgc cagatcagcg ccgtgaacag
     1801 gccagacacc atcgcggtga tgacggtgag cgcggccggc gagagacaga cgtcgtccaa
     1861 tcaactgacc tccctcgcga tcaccgtccg ctcgacctcg ttactgatct tcgggacgtc
     1921 tgcgacttcg taggtcacgc cgttgcagac gatccggtca gccggcgtca cgtcggtccc
     1981 ggccggcagc ttgatccgcc gttgcccgat cgccgtgatc gaggcgtccc cgccaagttg
     2041 ctcattcccg ccctggccga tccgagagac ccggcacgaa accgtcgcga cggtggccca
     2101 ggagtcggcc tgcccgtccc cgctctgggt ctgcgtgttc cgctggatcg tgcaggtatc
     2161 cgggcggaag ccggcggcca ggttgcggag cgacccgagc gggatggtca tggcgcggtg
     2221 tgctcgccac ggggagccgg cgcgtggtac tcggtgccgt cgcccatgct gacgatcttg
     2281 aagccggcct cttcgtacgt gaccatctcg ccggtcttcg ggtctttgta gtgcttgccc
     2341 cggcggtagt cggacgagtc gatgccgtac tgcgcgcccg ttttggggtg ctcgatgatg
     2401 atgtctggca tggtggcctc ctacgcgatg acgaaggatc gacggccgcg caccatctcc
     2461 agcgcgcgac tcacggcggg cggaacggcc gacgcatcgg cgtacttcac ggagatatcg
     2521 ttctgaccga ccgcgatctg cgagacgccg gccgcctccg ggtgcagcgt gaccgtcatc
     2581 aggtcgcttg ccaggatcgt cgcggccagc gcgatctcgg aggggatggc cagcgtgtag
     2641 gtgtaggaga cctgcaccag cgccgctgac gatgcgctcc agccctcgat cagcagcagg
     2701 ccattggccg cgtccagcag ctcgtactga ctcgcgtcga gcgtcgtcca gtcgaatgcc
     2761 gtgaacgccg ccgcccgggt cttcacgctg gtgacggcga ccaccggccg atggttcagg
     2821 tagacccgat tccccgtcag cgtgtggagc tcgtcggtgg ccgggctggc ctgctgccag
     2881 gactgattag tctcccggtc gatccaatcg ctgatggcct gggccacgag cgcggcctgc
     2941 gtctgctggt cggcggtcag cgtcgtcccg agataggcgg cgatgttggt ggcgttggtg
     3001 taggccgtca caggctcttg tcctccgccg gtcggatcat cttgtcggcg tagttgcgga
     3061 cccgcttctg gaagggcttc cggtcgagca ggctgtcccc ctcgacctcg atcagcgtgc
     3121 cctcgtactg attcgggatc tcgtcgatga cggtaccggt ggggagggag tagcccgagc
     3181 catcagggtt gatgactcgg atgccgtccc cgcccacccg gtacagcggc atcgcttaga
     3241 cgcctgtcac acgcgcgaag gcggttggac ggaacacgac aaacgccgcc ctcatttcag
     3301 ccaggattgt ctgcatgtta cgaataaatt gatcattgat cagccccacg cggatggtgg
     3361 actgctcgcg gtcgaacaac atgcacccga gctggaagtc gcccaccagc atggtgttgt
     3421 ccaccatgcc gatcgcctcg acgaccggcc gcccccagag ggtcatcggg ccggacacgc
     3481 tcggcgggcc gtacaggtag ctgccggggt tgacgttgcc cgtcacggcc gactcgcgca
     3541 tcagccgcac ggccgcccag tccacgggat cgaatacgaa ggcgttcggg ttggccagcc
     3601 ccgtgacctg gacctggacc attgcggcgt acgcggcgtc caccacgttg gcctgaccgc
     3661 cgtaggtggc gcccgccgcc aggccgagcg tctggatacc cgagccgagg atgccggcca
     3721 ggttcggggt gttcccgtcg ccgttgagga tctcggtctc aaggcgctgg ttcagcccgt
     3781 agacgagccg gttgtcgatg atgccccgga tctgcggcgc gtccgagagc atctgattcg
     3841 tgaccggaat ccagtgggcg atcgtctgca ccgggctggt cgcaagctcg aagttcagca
     3901 cggactcggg cttggtgccg gtggtgccgg tcgtcgccgt cgcctccgcg acttcggcgg
     3961 cgttgttcgt gaaggtcttc tccttcacgt actcgatcat gttcgacgtg gtctgggtcg
     4021 tcgggatcag gtccaacagc gtcaactggc gctgcaagat ctcctgatac cccggcagcc
     4081 ggtcattcgt gatcaggttg ccgccctgac ccgaggccga gtagaccagc gccttgcgcg
     4141 ccagagtcgg caacagcgtg cccttgaact ggacgcccag atcaatccgg ctctgggggt
     4201 tgttcagcac gcccgactca atgatccgct tgtactcggg gtcgctgatg aactgctggc
     4261 cgatcatggt cagcacgttg gacttcgcct cggcagcgtc gccctggggc tgctggtggc
     4321 cgttcaccgg tcggctgtgc ttggcgacgt tgtcgcggat gcgctgcgcc cggttgccgg
     4381 cctcttcgag ctcctgggtg cgggtctcga tcagatcgat ctcggagagc aggcgcttgg
     4441 tctgggtgta gtcctcgccg gcgtccggcg tgacgccgtc cgggtacttc ttctcgatgg
     4501 cggcggcctg ttcccagcgg ctcttgctct cggccatgca ctgcgagagg ttcatgttgc
     4561 gaaccttctc gacggtcagg atgttgtctg cagttgcggt gctcatgatg cgggtcgctc
     4621 caggattccg tgccgcgcga ggcggcgacg ggtgagctcc atcccgaggt tgatgcgctc
     4681 gggtgaggct ggcggaacga gcagcgcttc gacctcatcg gcagcgtttc ggagcgaccc
     4741 gctcatggct gctatgagtg ccttgcgcgc ttcggagaga gctcggccat cggtggcgcg
     4801 gatgtctgac ccagacttca cgcgcgagag ccactcggag acggtcgcct tgacggcctc
     4861 ggagtgctgc tcgaatggac ggccgagcga cttgacgtca gtgatgacgg cgttgcggtt
     4921 cgccggaatg gcgaccgccg aaacctcaaa gagatcgcac ttccggagca gacggacccc
     4981 gtcctcccgg tagtcggctt cgagggtgaa gtagccaatg ctcagactgt cgaggacgcc
     5041 actcttgacg agcttatagg cgtcctcgcc ggctcgggtg tccacgatcg accagcggcc
     5101 gaagagcccc tcatcgttct cttcgatggc aatctgcttg ccgatcggct ccatgtgctc
     5161 atagaggaat ttggtggcac gcttggcgat ggactcagcg aaggcgccac ggacgacgac
     5221 gtcaccgtag gagtccggct cgccgcccca ggtcgatgcc agtccggcga cttcccagcc
     5281 gccgccctcg gtcgggacga cctctttgac ctcgaagggg atcgacttgt attcgatcat
     5341 gaaaagagcc cccttcccct cgccgtgtcg cgagtggttg ggggctcagt ggcccgtgcg
     5401 tatcaggttg ctatcagtct acgctatcgc ttcctgtcag gcaactgctg cacctgatac
     5461 cgtttgcagc gccggtccgg gcagacggtc tcgacgcgcg cccggtccgt gtcgatcttg
     5521 cagatcagcc gcccgcagtc gcggcagcgg acctcgatca tccgcgagcc cggcggcggc
     5581 tcacgccacg aagacgcggc cacgcgcgct ctccgcgatc tgctgccatg cgctcggcca
     5641 gcgccagagg ttgccggcca ggctgtgccg cttctccacc gtcttcagga gccggccctg
     5701 catcagccgc cgcagcgacg gccgcgccac cagcgccgcc agccccgact cccactcgtc
     5761 cacggtctcc gcgatgaagc cggacaggtt gttctccacc gtccccgcgt agaacgacga
     5821 cgccaccacc gccgcccccg ccgccgccga ctcgtacacc ttgatctggc tcttggcccg
     5881 gttgaacgcc gtgttcgcaa cgctcgcgca cgcgatgtcg atctgggcca agccctgcgg
     5941 gtagctctcc aagggcagcc acggcagcgt caccagacga tcgtctggca catgctgcgc
     6001 gatcaccggc gggtgccagc cctgcaccac gaacgtgacc tcggggtagc gccgggcgat
     6061 ccgtccccac gcctcggcca tctgctcgac gtcgcggtcg tggcgccggc cgccggccca
     6121 gccgatcgtc aggcccggga tatttcgctt tgtggcagat actatccccc ggaaccaggg
     6181 caggtcgatc gcgttgggcg ccacgatcac gggcttgctg gtgtagctcc tgacgatcgt
     6241 cgccagccgc tgcgtcgaga ccgtcacccc gtcgcactgc tgcatggccc agatccgctc
     6301 gaagcgctcg gcctccagtt gctcgaacgt cttgccctcg gtccagccga gctccacccg
     6361 gcggtgggt
//
LOCUS       DASUGJ010000111         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1543932, whole genome shotgun sequence.
ACCESSION   DASUGJ010000111 DASUGJ010000000
VERSION     DASUGJ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543932"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFV93_02490"
     CDS             complement(<1..471)
                     /locus_tag="VFV93_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164240.1"
                     /translation="MNMLGLRHLDRRRHSLARALARSRAHSYAPRQGLATVMLLVAVL
                     LAGLGTAMPAQRVEAASFPVTVKLTIEHIDAIGDLDPGCGQADFYANASVNGVSGTQG
                     PIDGNDHPDPNWVFSAGATYDTGVSVPISIDLKEDDDTTCLGDDDIDINPGGGVG"
     gene            complement(795..867)
                     /locus_tag="VFV93_02495"
     tRNA            complement(795..867)
                     /locus_tag="VFV93_02495"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Thr,seq:cgt)
     gene            1535..1762
                     /locus_tag="VFV93_02500"
     CDS             1535..1762
                     /locus_tag="VFV93_02500"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX5164241.1"
                     /translation="MWHAHDGMGWWMLLGTTFWILFWVSIVWLLVNVIRPHREPVERD
                     DALAITRRRYASGEITHEEFERIRDDLTGID"
     gene            1847..2290
                     /locus_tag="VFV93_02505"
     CDS             1847..2290
                     /locus_tag="VFV93_02505"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX5164242.1"
                     /translation="MLRWTGIGIVVLLVAIQLIPYGRDHSNPPVTQPIAWDSARTEEL
                     AQRACYDCHSNETSWPWYSNIAPVSWLVQSDVDEGRHKLNFSEWGQPPRAAREAAETV
                     QEGEMPPWYYTILHPDAKLTDQEKQDLIDGFHATFGTGSQANDGN"
     gene            2315..2488
                     /locus_tag="VFV93_02510"
     CDS             2315..2488
                     /locus_tag="VFV93_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164243.1"
                     /translation="MGGTATAPEIERYRPKLQAEIDGAALYRVLSDMDGSAELAKVYD
                     RLATTEAGHATYW"
     gene            complement(2796..>3233)
                     /locus_tag="VFV93_02515"
     CDS             complement(2796..>3233)
                     /locus_tag="VFV93_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5164244.1"
                     /translation="LAADAVSTAPPEQLQRHIEEAYAFLAHHLIPHAHAEEEALYPVV
                     AQLMGAPEATATMRRDHVEVGQLTEELNSLRRSLATSAAPTPELEQALRRVLYGLYAV
                     VTLHFAKEEEVYLPLLDARLTPDTARTMFAAMHAAAHHAAALH"
BASE COUNT          633 a          990 c         1005 g          605 t
ORIGIN      
        1 cccaacgcca ccgccggggt tgatgtcgat gtcgtcgtca ccgaggcagg tggtatcgtc
       61 gtcttccttc aagtcgatcg agatcggaac gctcacccct gtatcgtagg tggcgccggc
      121 actgaacacc cagttcggat cgggatgatc gttaccgtct atcggacctt gcgtcccgga
      181 gacgccgttc accgaggcat tagcgtagaa atcggcctgc ccgcaaccag gatcgaggtc
      241 accaatcgcg tcgatgtgct cgatcgtcaa cttcacggtg accgggaagg acgcggcttc
      301 aacccgctgc gccggcatcg ccgtgcccag cccggcaagc aacaccgcaa caagcaacat
      361 cacggtggcc aggccctgcc gtggagcata cgagtgggca cggctgcgag cgagtgccct
      421 cgccagactg tgccgtcgtc gatcgaggtg ccgcaaaccc agcatgttca tcgtccctcc
      481 atagatgccg attggtccac tgcattggtg atagagatcg attgccctcc ggtcgctctc
      541 ccttcctaaa cgacgacggc cccagcacac gcgtacggcg tgcagaggtc gcgctcgtca
      601 ggttcgtcgt gcctggccgc gctgggccct gcacattcga catcagaacg aggcgtgatc
      661 tgattgtagg agggagatat ttgcagaacc tttgcagcgg ttgagcacgc gacggaaacg
      721 atcaatcgcc acccgcgaca cggaaacgcc ggccgctacg gccggcgatc gtatcaacct
      781 ttcgatcttg agggagccga cgaagggatt cgaacccttg acctgctgtt tacgaaacag
      841 ctgctctgcc agctgagcta cgtcggcaca ggtctccgca tcagaatgcg gaaactggta
      901 tcgagttggg ccagtcgtcg cactatttgt ctgcacagtc cgacgctgaa cgcgagcata
      961 gcataccaaa tccacgcgga attccgccac gcgggcaccg aggcggttga aatcgatgcg
     1021 gaaataggtg aatagcgctg acatcaccgc gctgatatct ggctaactca gcgctcaatg
     1081 gtgagaaatc acgcgttatt cgatcggagc ctgcctctca tgacctcact gagacattga
     1141 acgtaggaag ccattctgta gagacgcgcc cgtattcaac gatcgtgacg cggcgggtcg
     1201 tcagagtctt tgtcaaatac tctcgccctc gccactcaat catctgcctg acggcgacct
     1261 gcgacgccta acgcgactgg ctgtcaacgg cttgccagct gcacgcctcc ggtttgcgac
     1321 gctgaaacgg ccctgtggcg catgatgagc acaggcgtta ggaggtcagt ttcgttccgg
     1381 cggcgtcggt cgcgccgcgt acgtcggcat gctggcctgc ccgatcacgc catccgggcc
     1441 gacattgaca tgaagctgca acacggcgac cgaccgcgat gacgcgggtc taacgcctgg
     1501 cagtccagac tggatcgagc gaagggagaa tttaatgtgg cacgcacatg atggcatggg
     1561 ctggtggatg ctgctcggaa caacgttctg gatcctgttc tgggtctcga tcgtctggct
     1621 gctcgtgaac gtcatccgcc cccatcgcga gccggttgaa cgtgacgacg cgctcgcgat
     1681 cacgcgtcgt cgctacgcct ccggcgagat cactcacgag gagttcgagc gcatccgtga
     1741 tgaccttact ggcattgatt gatcgactgg aaaggtccgt ctgatggtcg aggacacgat
     1801 ggtcaaggag ccaactcgag agcgccgcca catggattgg ttccgtgtgc tccggtggac
     1861 aggtattgga atcgtcgtac tgctggtggc gatccagctc attccgtacg ggcgcgatca
     1921 ttccaacccg cccgtgacac aaccgatcgc gtgggatagc gctcgaactg aggagttggc
     1981 ccagcgcgcc tgctacgact gtcatagcaa cgagacgagt tggccatggt acagcaacat
     2041 tgcgccggtc tcgtggcttg tccagagtga tgtcgacgag ggccggcaca agctcaactt
     2101 ctccgagtgg ggccagcctc cacgagcagc acgcgaggcg gccgagacag tacaggaagg
     2161 cgagatgccg ccctggtact acacgattct gcatcccgat gccaagctga cggaccagga
     2221 aaagcaggat ctgatcgacg gcttccacgc aaccttcgga accgggagcc aggctaatga
     2281 cggtaattga catggactat cggggcgttc agtgatgggt gggactgcca cagcacctga
     2341 gatcgaacgg tatcggccga agctccaggc tgagatcgac ggcgccgcgc tctatcgtgt
     2401 cctgtccgat atggatggga gtgccgaact ggcaaaggtc tacgacaggc tcgcgacgac
     2461 tgaggcggga cacgcgacct actggtagga aaagctccgt caatcgggtg cggagcccag
     2521 gaagccgcgg cccgattggc ggacgcgggt gctgatcgcc ctggcccgcc gcttcggcac
     2581 ggcgctgatc ctgccgaccg tcactgtgca cgagcagtcg gatagtgacc gctatctcgg
     2641 tcagccggat gcgagtgcgg ccgggctcgc gtcggaggct ctaatcgctc tccaaggtgt
     2701 gcgcgaactc gtgctcctcc gcttgggaga cagctgctgg ttctgttcgc tgggtgccga
     2761 gctaatccgc gaacaaccag ggaccagcag gtcgcctagt ggagcgcggc agcgtggtgc
     2821 gcggcggcgt gcatcgcggc gaacattgtt cgggcggtat cgggcgtcag ccgggcgtca
     2881 agcagcggca gatagacctc ttcctccttc gcgaagtgca acgtcaccac agcgtacagc
     2941 ccgtagagca cgcggcgtag tgcctgctcc aactccggag tgggcgccgc actggttgcc
     3001 agacttcggc gtagactgtt cagttcctcc gtcagttgac cgacttccac atggtcacgg
     3061 cgcatggtcg ccgttgcctc gggcgctccc atcagctgcg cgacgacagg atagagcgcc
     3121 tcctcctccg catgcgcgtg cggaatcagg tgatgggcca ggaacgcata ggcctcctcg
     3181 atatgccgtt gcagctgctc cggcggagcg gtgctcaccg catccgccgc cag
//
LOCUS       DASUGJ010000112         6151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_709228, whole genome shotgun sequence.
ACCESSION   DASUGJ010000112 DASUGJ010000000
VERSION     DASUGJ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709228"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="VFV93_02520"
     CDS             complement(<1..1467)
                     /locus_tag="VFV93_02520"
                     /inference="COORDINATES: protein motif:HMM:NF004801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5164245.1"
                     /translation="MPDRWRGMTTLLRGRWVIASDGDSHHILEDGEVVYDGNSIIFTG
                     HGYDGPVDETIDAGNAVIGPGFVDLDALFDLDSTILGFDNHPGWAKARVWASSYVERG
                     PRDVYSDEEEDFQHEYALGHLLLNGITTALPIRSILYREWAETYDENARAADAAARLG
                     IRAYLGPSYRTGLPMVYPDTSISMHWDIERGMRGLDEAIRFVRDFDGAHDGLIRGFLQ
                     PDRIEGCTEELIVRSGEAAEELDCPIRLHCCQGELEVQEVHNRWGKSSLTLLHDLGFL
                     SRRALLPHGIYLGGVEPTAEQVEREIGWLAESGATIVHCPLVSARSGNVMDSFSAFRE
                     AGVRIGLGTDTWPSDIVLNMLTGVIATRIREGGPRVTAADYYHAATIGGADALNRPDL
                     GRLMPGALADITVYDLSNPRTGQFIDPIQTMILNGTGRDFTTVIVNGRTVVRDGQLPG
                     VDLDAWHARAQRQYDTLIASYPERAHLHPPVEEIFAPSF"
     gene            complement(1443..2645)
                     /locus_tag="VFV93_02525"
     CDS             complement(1443..2645)
                     /locus_tag="VFV93_02525"
                     /inference="COORDINATES: protein motif:HMM:NF004636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5164246.1"
                     /translation="MADELLITNVRPLGRDTVDIHVVDGKIASLERGTKQPDATAING
                     GGRLLFPGFVDAHTHMDKTLLGMGWYRNEVGPSLVDKIENERRLRREQGIDSHQQSSR
                     HARIAVATGTTHIRTFVDIDTEVELGGFEGVMQTREDFKDALDIQVVAFPQSGMLIRP
                     GTVELMEDALRNGAQVVGGLDPSSIDRDPARHLDVIFELAQKYDADIDIHLHEPGELG
                     AFSVELIAERTKALGMQGRVVISHAICLGGIDDAYLDRLIELLLENRITIMSHGPGGL
                     RPVPSVMRLRKAGVPMCTGNDGIRDAWGPLNMPDMLLRTFIIAYRNNLRRDDEIEEVL
                     DIATYGGARVIGDDNYGLAVGCNADFVLLDGETHVEAVIERPARWLVVKGGQIVAREG
                     ECLIGGAA"
     gene            complement(2680..3321)
                     /locus_tag="VFV93_02530"
     CDS             complement(2680..3321)
                     /locus_tag="VFV93_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5164247.1"
                     /translation="MAIQGMIFDFDGTLGDTLQLCCAAFREVTQRRLGRDFSDAEIMA
                     LFGPSEDGIFQQLAGDGWQDCHADFLAIYERDHATSAELFPGIPEMLAVLRERDIPAA
                     IVTGKSAESAAISLRQLELAAHFDIVEAGSPEGSVKPVAIRRILERWKIDPATVAYVG
                     DAPNDVSEAHAAGVIPLAAAWAATVDRAALEARKPAATFASPADLTTWIRHNA"
     gene            complement(3303..3887)
                     /locus_tag="VFV93_02535"
     CDS             complement(3303..3887)
                     /locus_tag="VFV93_02535"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5164248.1"
                     /translation="MSRPALVIVSGLPASGKTTLARRLAHELRLPLLARDDIKERLFD
                     QLGWSDRDWSRRLGGASWELLYWAIEAQLAAGRSCIAESNFHPERDRRRFRELGQRFM
                     FTPIEIHCYATGSVLVERYLARIANGSRHPGHVDHVTIDEQREQLLAAAPEPLALGGA
                     TLLVDTTDPSTIDYDSLVNEVRSMLTEAEWRSRG"
     gene            complement(3884..4336)
                     /locus_tag="VFV93_02540"
     CDS             complement(3884..4336)
                     /locus_tag="VFV93_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5164249.1"
                     /translation="MSEASHCLIDSNVLIYMHDDKRASHQKQAVDIYRRLVESNRSVL
                     STQCLVEFFNAVTRKLRDPLSIAEAVRRLNQIADTAVVYPVTLDIVKDAAIAAEMYQM
                     SIWDAQIWAVAFRNGVPIVVTEDMQSQPIIAGVRYINPFDPAFDIQSL"
     gene            complement(4333..4680)
                     /locus_tag="VFV93_02545"
     CDS             complement(4333..4680)
                     /locus_tag="VFV93_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164250.1"
                     /translation="MAQMIRKQIYIDADNARFLKERSSELGVTEAQIVRQALADAERK
                     ACAEAEQRAKDEKRLAALDNILTYIREHRMSADPSSDDSQGGWVFNRDELYDERMDRY
                     GPQRDEEPEQYGG"
     gene            4813..5343
                     /locus_tag="VFV93_02550"
     CDS             4813..5343
                     /locus_tag="VFV93_02550"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5164251.1"
                     /translation="MRNTLLALLAEGSGHGYELKQAIEQTFGDAMPQINIGQVYTTLG
                     RLERDGLVASAHVAQAGRPDKRVYELTEMGREEVRRWLAEPVNGPRLKDEFFTKLVLA
                     RLPGVAALNGGTDARALIARQRRDYLQSLRDLNGLLLRQSRDGGPTSAELLIEGAILH
                     LEADLKWLDLCEQRLT"
     gene            5354..6100
                     /locus_tag="VFV93_02555"
     CDS             5354..6100
                     /locus_tag="VFV93_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5164252.1"
                     /translation="MKDNAVVLEARGLTRTFTTGDLPVHALRGVDLALEPGSFTAIMG
                     PSGCGKSTLLHLLGGLDRPTAGEIHLAGSRVDQLSEARWSVLRRREIGFVFQFFNLIG
                     NLTAADNIELPSLLVGTSHAEARRRSQELLGELGIGDKAGAIPARLSGGQQQRVALAR
                     ALVNNPTLLLADEPTGNLDSESTRDVLTLLQRLNAGGQTILLVTHDARVASIAGRVIT
                     MRDGTIVDDTRLSSDEPSAELLAHLVRLEA"
BASE COUNT         1047 a         1941 c         2047 g         1116 t
ORIGIN      
        1 aaacgacggc gcgaagatct cctcgaccgg cggatgcaaa tgcgcgcgtt ccgggtacga
       61 cgcgatcagt gtgtcgtact ggcgttgcgc acgcgcgtgc caggcgtcca ggtcgacgcc
      121 gggtagctgg ccgtcgcgga cgacagtgcg tccgttgacg atgacggtcg tgaagtcgcg
      181 gccggtgccg ttgaggatca tcgtctggat cgggtcgatg aactggccgg tgcgtgggtt
      241 ggagaggtcg tagacggtga tgtcggccag cgcgccgggc atcaggcggc ccaggtcagg
      301 gcggttgagc gcgtcggcgc caccgatcgt ggcggcgtgg tagtagtcgg cggcagtgac
      361 gcgcggcccg ccctcgcgga tgcgggtcgc gatcacgccg gtcagcatgt tgaggacgat
      421 gtcggacggc caggtgtcgg tgccgaggcc gatccggacg ccggcctcgc ggaaggccga
      481 gaacgagtcc atgacgttcc cgctgcgggc cgagacgagc gggcagtgga cgatcgtcgc
      541 gcccgactcg gccagccagc cgatctcgcg ctcgacctgc tccgcagtcg gctcgacccc
      601 gccgaggtag atgccatgcg gcagcagtgc ccggcggctc aggaagccga ggtcgtgcag
      661 cagcgtcagt gacgacttac cccagcggtt gtggacttcc tgcacctcaa gctcgccctg
      721 gcagcagtgg agacggatcg ggcagtccaa ctcttcggcc gcctcgccgg atcggacgat
      781 cagctcctcg gtgcagccct cgatcctgtc cggctggagg aagccgcgga tcaggccgtc
      841 gtgggcgccg tcaaaatcac ggacgaagcg gatcgcttcg tcaaggccgc gcatgccgcg
      901 ctcgatatcc cagtgcatgc tgatgctcgt gtcggggtag accatcggca ggccagtccg
      961 gtaggacggg ccgagatagg cacggatgcc gagccgggcg gcggcatcgg cggcgcgagc
     1021 gttctcgtcg taggtctcgg cccactcgcg gtagaggatc gagcggatcg gcagcgccgt
     1081 cgtgatgcca ttcagtaaca ggtggccgag cgcgtactcg tgctggaagt cctcttcctc
     1141 atcgctgtag acatcgcgcg ggccgcgctc gacgtagctg ctggcccaga cccttgcctt
     1201 ggcccagccg ggatggttgt cgaagccgag gattgtcgag tcgaggtcga agagtgcgtc
     1261 gaggtcgacg aaaccggggc cgatgacagc gttgccggcg tcgatcgtct catcgaccgg
     1321 gccgtcgtag ccgtggccgg tgaagatgat ggagttgccg tcgtagacga cctctccgtc
     1381 ttcaaggatg tggtggctgt cgccgtcgct ggcgatcacc cagcgcccgc gcaggagcgt
     1441 cgtcatgccg cgccaccgat caggcactcg ccctcgcggg cgacgatctg gccgcccttg
     1501 acgaccagcc agcgggccgg ccgctcgatg acggcctcga cgtgcgtttc gccgtcgagc
     1561 aggacgaagt cggcgttgca accgacggcc aggccgtagt tatcgtcgcc gatgacccgc
     1621 gcaccgccgt aggtggcgat atcgagcacc tcctcgatct cgtcgtcgcg gcgcaggttg
     1681 ttgcggtagg cgatgatgaa cgtccgtagc agcatgtctg gcatgttgag cggcccccag
     1741 gcgtcgcgaa tgccatcgtt gccggtgcac atcgggacgc cggccttgcg cagccgcatg
     1801 accgatggga ccggccgcag gccgcccggg ccgtgactca tgatcgtgat ccggttctcc
     1861 agcagcagct cgatcagccg gtcgaggtaa gcgtcgtcga tccctccgag gcagatggcg
     1921 tgggagatga cgacccgtcc ctgcatgccg agcgccttcg tccgttcggc gatcagctcg
     1981 accgagaaag cgcccagctc gcccggctcg tggagatgaa tgtcgatatc ggcgtcgtat
     2041 ttctgcgcca gctcgaagat cacgtcgaga tggcgggccg ggtcacggtc gatcgacgac
     2101 gggtcgagtc cgccgacgac ctgcgcgcca tttcgcagtg catcctccat caactcgacc
     2161 gtgccgggcc ggatcagcat ccccgactgc gggaaagcga cgacctgaat gtcaagcgcg
     2221 tccttgaagt cctcgcgggt ctgcatgacg ccttcgaagc caccgagctc gacctcggtg
     2281 tcgatgtcga cgaaggtgcg aatgtgggtc gtgccggttg cgacggcgat ccgcgcgtgg
     2341 cggctcgact gttggtgcga gtcgatcccc tgctcccgcc gcagtcgccg ctcgttctcg
     2401 atcttgtcga cgagcgatgg cccgacctcg ttgcggtacc agcccatgcc cagcagcgtc
     2461 ttgtccatgt gggtgtgggc gtcgacaaag ccggggaaca ggagtcgccc accgccgtta
     2521 atggcagtcg cgtcaggttg ctttgtgccg cgctccagcg acgcgatctt accatcgaca
     2581 acgtggatgt cgaccgtatc gcgtcccaac ggccggacgt tggtgatcaa cagttcgtca
     2641 gccatcactc atcccctcct cccgagtgtc agcgaacgtc tacgcgttat gtctaatcca
     2701 ggtcgttaga tcagcgggtg aggcgaaggt cgctgccggc ttgcgtgcct ccagtgctgc
     2761 tcgatctacc gtcgccgccc aggcggcggc cagtgggatg acgcctgcgg cgtgcgcctc
     2821 gctgacatcg ttcggggcat cgccaacgta ggcaaccgtc gcaggatcga tcttccagcg
     2881 ttcgaggatc cggcggatgg cgaccggctt gacgctcccc tccggtgagc ccgcttccac
     2941 gatgtcgaag tgtgctgcca gttcgagctg tcggagcgag atggcggccg attcggcgct
     3001 cttgccagtc acgattgcgg ccgggatgtc gcgctcgcgc aggaccgcca gcatctcggg
     3061 gatgccgggg aacagttccg cgctggtggc gtgatcgcgc tcgtagatgg ccaggaagtc
     3121 ggcgtggcag tcctgccagc catcaccggc aagctgctgg aagatgccgt cctcgctcgg
     3181 gccgaagagc gccatgatct cggcgtcgct gaagtcacgc ccgagccgcc gttgcgtcac
     3241 ctcgcggaac gcggcgcagc agagctgcaa cgtatctccc agcgtgccgt cgaaatcgaa
     3301 gatcatcccc tggatcgcca ctcagcctcc gtcaacatcg atcgaacctc gttcacaagc
     3361 gaatcgtagt cgattgtgga cggatcggtc gtgtcaacga gcagcgtggc cccgccaagc
     3421 gcgagcggtt ccggcgctgc cgccagcaat tgctcacgct gctcgtcgat cgtgacgtga
     3481 tcgacgtggc cgggatggcg cgagccgttc gcaatccgag ccaggtagcg ctcgacgagg
     3541 acgctgccgg tggcgtaaca gtggatctcg attggtgtga acatgaagcg ctgccccagt
     3601 tcccggaagc gccggcgatc gcgctcagga tggaagttgc tctccgcaat gcaggaccgc
     3661 ccggcagcca gttgtgcctc gattgcccag tacagcagct cccagcttgc gccgccgagc
     3721 ctgcgagacc agtcccggtc gctccagccg agttggtcga acagccgttc cttgatgtcg
     3781 tcgcgcgcca gcagcggcag gcgcaactcg tgcgccaacc gccgcgccag cgtcgtcttc
     3841 ccgctggccg gcagcccgga gacgatgacg agtgccggac ggctcatagc gactggatgt
     3901 cgaaggcagg atcgaacggg ttgatgtacc ggacaccggc gatgatcggt tgcgattgca
     3961 tatcttcggt aacgacgatc ggaacaccgt tgcggaatgc taccgcccaa atctgcgcgt
     4021 cccagatcga catttggtac atctcggccg ctatcgccgc gtccttgacg atatcgagag
     4081 tcaccgggta tacaaccgca gtatcagcaa tttggtttag cctccgaaca gcttcagcga
     4141 tcgaaagcgg gtctcgaagc ttgcgagtga ccgcgttgaa gaactcaacc aagcattggg
     4201 tactaagcac gctgcgatta gactcgacga gacggcggta gatatcgact gcttgtttct
     4261 ggtgggaggc gcgcttgtca tcgtgcatgt aaatgagaac attgctatca atgaggcaat
     4321 ggctcgcttc gctcatccgc cgtattgctc cggttcttcg tcgcgttgcg ggccgtagcg
     4381 atccatccgt tcatcataga gttcgtcccg attgaagacc cagccgcctt gcgagtcgtc
     4441 ggatgagggg tcagcactca ttcgatgttc tcggatgtac gtaagaatgt tgtcgagagc
     4501 agctaggcgc ttttcatcct tggcccgctg ttcggcctct gcacacgcct tacgctcggc
     4561 gtcggccagc gcctgacgga cgatttgagc ctcagtcacg ccgagttcgg acgatcgctc
     4621 cttgagaaag cgtgcgttgt cggcgtcgat gtaaatctgc ttgcggatca tctgggccat
     4681 ctcgaccacc ttcctataca tcacagtata catcgcggct tctgtgttgt aggggcttga
     4741 caagtcgcgt ctgatcaact acatactgag tatgtataac gagtatgtaa tcgcgcgtga
     4801 agcgacctgg agatgaggaa cacgctgctg gcgctgctgg cggagggctc ggggcatggc
     4861 tatgagctca agcaggccat cgagcagacc ttcggcgacg ccatgcccca gatcaacatc
     4921 ggccaggtct acaccacact cggccggttg gaacgcgacg ggctggtcgc cagcgcccac
     4981 gtcgcccagg ccggccggcc agacaagcgg gtctacgagc tgaccgagat ggggcgggag
     5041 gaggtccgtc gctggcttgc agaaccggtc aacgggccgc gtctgaagga cgagttcttc
     5101 acgaagctgg tcctggcacg gctgccgggc gtagcggcgc tcaatggagg caccgacgct
     5161 cgggcattga tcgcccgcca gcgacgcgac tacctccagt cgttgcgtga tctaaatggg
     5221 ctgctcctca ggcaatcacg cgacggcggc ccgacatcgg cagaactgct gatcgaaggc
     5281 gccattctcc acctcgaagc tgacctcaag tggctcgacc tgtgcgagca gcgactgacc
     5341 taaggagtcc agcatgaaag acaacgccgt cgtcctcgag gctcgtgggt tgacccggac
     5401 cttcacaact ggcgacctgc cggtccacgc gctccgcggc gtcgatctcg cgctggagcc
     5461 tggctccttc acggccatca tgggaccgag cggctgcggc aaatcgacgt tgctgcatct
     5521 gctgggtggg ctggaccggc cgacggccgg cgagattcac ctcgccggtt cgcgcgtcga
     5581 tcaactgagc gaggcgaggt ggtcggttct gcgccggcgt gagatcggct tcgtcttcca
     5641 gttcttcaac ctgatcggca acctgacggc cgccgacaac atcgagctcc cgagcctgct
     5701 ggttggtacc tcgcatgccg aggcccgccg ccgcagtcag gagctgctcg gcgagttagg
     5761 gatcggcgac aaggccggcg cgattccggc gcggctctcg ggcgggcagc agcagcgggt
     5821 cgcgctggcg cgggcgctgg tcaacaatcc aacgctgttg ctggcggatg agccgaccgg
     5881 caacctcgac agcgagagca ctcgcgacgt gctgacgctg ctgcaacggc tgaacgccgg
     5941 cgggcagacg atcctgctcg tgactcacga cgcccgtgtc gccagcatcg ccggacgggt
     6001 catcaccatg cgcgacggca cgatcgtcga tgacacgcgc ttgagttccg atgagccgtc
     6061 ggctgaactg ctggcgcacc tggtcaggct ggaggcgtag gatgtttcgc gcgatgtggc
     6121 gcaaggcgct ggccgacacc cgcagtcacc g
//
LOCUS       DASUGJ010000113         5911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_849094, whole genome shotgun sequence.
ACCESSION   DASUGJ010000113 DASUGJ010000000
VERSION     DASUGJ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849094"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..617)
                     /locus_tag="VFV93_02560"
     CDS             complement(12..617)
                     /locus_tag="VFV93_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5164253.1"
                     /translation="MVVGSTTIEARITDFPFDVTDQMLMQRLLRRDLSALEELINRHR
                     SVAIGLACRIVHDRNHAEDVVQDAFLTLWRQPERYDPARGSVRSWLLAIVHHRSIDKL
                     RRLATNGRLVELTPDIVDHSAVDPSEQAVQAVERDNVRAALDRLPADQRRAIELSFLH
                     GRTHAEIAELMNCPLGTVKGRIRIGLEKLRTMIQPTDLVAA"
     gene            complement(779..2311)
                     /locus_tag="VFV93_02565"
     CDS             complement(779..2311)
                     /locus_tag="VFV93_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5164254.1"
                     /translation="MIINDVSQVDDFQDIPAVTVFGMRAYIGVPIVLADGGIYGTLCA
                     LDRTPQQKTRRDLDMLLILARLLASQLDRQQIGALEERQRIAREIHDTLAQSLASLTL
                     DLSLHTAQLGQVAPHLVAESQRMLDATRDALREVRRSIWNLQPGALQGKSLTEAIGIE
                     LRETLRAGIDASIELRGPSGPLPPPIEATVMRIAQEALANVRKHSRASQVIVTLEYLP
                     DALCLHVDDDGRGIDLAGLAPTSEAGGFGLASMRERARQAGGDLRISLRPGGGTSLFC
                     RIPRLVAAPAIETAPTTLQPPDPTRRQVRVGIIDDHAVVREGLIRLLGSASGITVSWQ
                     APNAETGLELIARAAPEVLLLDLQMPGLGGLGCLERLASTRSPVRTIVLTTFALDEMV
                     FQAIRLGARGYLLKDASPDDLIDAVRTVAGGGTLLAPVAAEQLAQRVNHPETLTPRER
                     EVLALLADGLRNKEIALQLGTSEKTVQFHIANLFGKLGVSSRTEAARVALERGLLASR
                     IA"
     gene            complement(2339..2599)
                     /locus_tag="VFV93_02570"
     CDS             complement(2339..2599)
                     /locus_tag="VFV93_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164255.1"
                     /translation="MAGEREVAADADAPATLAAVSQLAARSFADVGEAIDATLTLMSD
                     LLDMEVRMVNRVEADRHTFTRLQLPDDMPALEGLSTPLNHNF"
     gene            2842..3504
                     /locus_tag="VFV93_02575"
     CDS             2842..3504
                     /locus_tag="VFV93_02575"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX5164256.1"
                     /translation="MDDETTADLTARYAKVYSAAVADVLDKRGYRNQVVSGAIQGLTL
                     ETRVAGPAYTCKGAPATALEPDDWDMRKQFLDNVRPGTVVVVDTSDDTASAHWGELMS
                     TAARGRGATGVVIDGATRDVTQMLAMGFPAFVRYRSPASSILRWRISGYDHPVTIGGV
                     LVRPGDFVVGDADGVVIVPVELAVEVLEEVESLAVAETNMRQELLEGGTFSDVFDRYQ
                     VG"
     gene            3497..3850
                     /locus_tag="VFV93_02580"
     CDS             3497..3850
                     /locus_tag="VFV93_02580"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX5164257.1"
                     /translation="MADTTGSQQRMVAALGYVFTPVVPLITLTSDLKQDPFMKRHAGQ
                     ALLWSAGLVLGFVVIVIAAIWMLRTELLLICLFPALFLLPFLPGAWWGWRVYQGGDPR
                     PPLIAPLAARLFPAR"
     gene            complement(3810..5030)
                     /locus_tag="VFV93_02585"
     CDS             complement(3810..5030)
                     /locus_tag="VFV93_02585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5164258.1"
                     /translation="MSIPGWTAVVVNYNGAGYLDACLRALEQTQPAPVEIVVVDNAST
                     DDSLQELHSFPRVHVLAQPANLGFAGGANVGLTAVETPFALLMNPDVELDPDFGSALL
                     AEFAADEKLGAAGPLLRYPDSDRIQHAGGVIEQPLLATAHLAYEQRIGETQLAETDVD
                     FVTGGAMGLRMSAFQEIGGFDDVFSPVYYEDVDLCVRLRATGWRVRFLPSLRAVHHEG
                     VTLERSDAYHQHLHRNRIRFALKHLSGRQWRTSFVPAEYARLRHELHTLIEDGWPARS
                     GAAAVESLLRGYENREGWDAPAALHAPPPALLELRIDAARELARPRDDAPAGMRSLGL
                     RVLGLVRDVGLRRQVQIALDQQRHFNDAIIHTLEAQDAINREQTAMTLLLALDLLGRL
                     QTNVPEKAAPQAEQ"
     gene            5097..>5911
                     /locus_tag="VFV93_02590"
     CDS             5097..>5911
                     /locus_tag="VFV93_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164259.1"
                     /translation="MDNLRPSVLLIASDTIGERMAGSGIRYWNLARVIGARQPVTLAT
                     PNETTLPAPAGVTLVPYLGSSDERGRQLAELVGAHVVVVAQHLPYLYTDADVLASRAL
                     VVDLYAPWILEKLEYSRVDPERGEIDRKDDVTILNRLLALGDFFICASERQRDFWLGA
                     LAAAGRLDLTHAQAGPDLRTLIDVVPFGLPPDKPVRNGTGPRGTFDGIGDNDIVALWN
                     GGLWNWLDPLPAIRATAQVAAAEPRFRLVFMGTQSPGAQVAEMEIVSTARDLA"
BASE COUNT          946 a         1906 c         2014 g         1045 t
ORIGIN      
        1 ggacatggtg tttacgcagc gacgaggtcg gttggctgga tcatggttcg cagcttttcg
       61 aggccgatcc ggatgcgtcc tttgacggtg ccgagcggac agttcatcaa ttcggcgatc
      121 tcggcgtggg tgcggccgtg caggaacgag agctcgatcg cccggcgctg gtcggctggg
      181 agccggtcga gcgcggcccg gacgttgtcg cgctcgactg cctggactgc ctgttcgctt
      241 ggatcgactg ccgagtggtc gacgatgtcc ggtgttagtt caacgagacg gccgttcgtt
      301 gccaggcggc gtagcttgtc gatgctgcga tggtggacga tcgccagcaa ccagctgcga
      361 acgctcccac gggccggatc gtaccgttcc ggttggcgcc agagtgtcag gaacgcatcc
      421 tggacgacat cttcggcgtg attgcgatcg tggacgatcc ggcacgcgag accgatcgcg
      481 accgatcgat gccggttgat cagttcttcg agggcagaca gatcgcgccg tagcaggcgc
      541 tgcatgagca tttgatcggt gacgtcaaag ggaaaatcag tgatcctcgc ctcgatggtg
      601 gttgaaccga cgaccatcct gctgactcct tatccacgct cgtccagccc tggccggccg
      661 gacgagacaa acggcgtgcg gatcgtgctg tgtgcagtgc ttcgatcgta gataccgctg
      721 cgtcgttgcg acacgggagg agccccgggt ctggacctgg gaaaaaccct gggatcgctc
      781 aggcgatccg tgatgcaagg agcccacgtt cgagcgccac gcgcgccgct tcggtgcggc
      841 tgctgacgcc gagcttgccg aacaggttgg cgatgtggaa ctggaccgtc ttctcgctgg
      901 tcccgagctg gagcgcgatc tccttgttgc gtagtccgtc cgccagcagc gcaagcacct
      961 cgcgctcgcg cggcgtcaac gtctcgggat ggttgacacg ctgcgcaagc tgctcggcag
     1021 cgacaggggc gagcagtgtc ccgccgccgg caacggtcct gacagcgtcg atcaggtcgt
     1081 ccggtgaagc atccttgagc aggtagccgc gcgcgccgag ccgaatcgcc tggaagacca
     1141 tctcgtccag ggcgaatgtc gtcaggacga tcgtcctgac cggcgatctg gtcgacgcca
     1201 gccgctccag gcagccaagc ccgccgagtc ccggcatctg gagatcgagt agcaggactt
     1261 cgggcgctgc gcgggcgatc agttccaggc ccgtttcggc gttgggagcc tgccagctga
     1321 cggtgatgcc gctcgcgcta ccgagcagcc gtatcagccc ctcgcggacg acggcgtggt
     1381 cgtcgatgat gccaactcgc acctgacggc gggttggatc aggcggttgg agcgtggtcg
     1441 gagcggtttc gatcgctggc gctgccacga gccggggaat ccgacagaac agagacgtgc
     1501 cacctcccgg ccggagcgag atccgcagat caccgccggc ctgtcgcgcg cgctcccgca
     1561 tgctggccag gccgaacccg cctgcctcgc tggtcggtgc caggccagcg aggtcgatgc
     1621 cccggccgtc gtcatcgaca tgcaggcaga gggcgtccgg caggtactca agcgtgacga
     1681 tgacctggct cgcccggctg tgctttctga cgttggcgag tgcctcctgc gcgatccgca
     1741 tgacggtggc ttcgatcgga ggcgggagcg gtcccgatgg gccgcgcagc tcgatcgacg
     1801 cgtcgatgcc ggcccgcagc gtttcgcgga gctcaatgcc gatcgcctcg gtcagcgact
     1861 tcccttgcag cgcgccgggt tgcaggttcc agatactgcg gcgcacctcc cgcagggcat
     1921 cgcgggtcgc gtcgagcatc cgctgtgact cggcgacgag gtgcggcgcg acctggccca
     1981 gctgggccgt gtgcaacgac aggtcgagcg tgagcgacgc cagcgactgt gccagcgtgt
     2041 catgaatctc gcgcgcgatg cgctgccgct cttccagcgc gccgatctgc tgccggtcca
     2101 gctgtgatgc cagcaatctg gccaggatca ggagcatgtc gagatcgcgg cgagtcttct
     2161 gctgcggcgt gcggtcgagc gcgcagagag tgccatagat gccgccgtcg gcgaggacga
     2221 ttgggacgcc gatataggcg cgcatgccga agacggtcac ggccggaatg tcctggaagt
     2281 catcaacctg cgagacatcg ttgatgatca tgggctcgac cgagccgatt actcgccgtc
     2341 agaagttgtg gttcagcggg gttgagagtc cttcgagagc cggcatgtcg tccggcagct
     2401 ggaggcgcgt gaaggtgtga cggtcggcct cgacgcggtt gaccatccgg acttccatgt
     2461 cgagcagatc gctcatcagt gtcagcgtcg cgtcgatcgc ttcgccgaca tcggcaaacg
     2521 atctggccgc cagttgcgag actgccgcca acgtcgccgg tgcatcggca tcggctgcca
     2581 cctcacgctc gcctgccatc ggaaaccctt ccagttccgc gactgggacg acgccacgcc
     2641 gaacaactcc ggcctggcac gcgtattcgc aatgcttcgt gagcccattg taacagcgcg
     2701 cgagcagcgt cctcaatctt gagcgatgtt gcacgaaaga ggatggctgc tgcatcgtgc
     2761 cgctggaggc aaggttgccg ggcaagtggc cctgcacgta gactcgcgcg aacggatggt
     2821 tgcccgtggg aggtggattg agtggacgac gagacgacag cggatctgac ggcgcgctac
     2881 gccaaggtgt acagcgctgc ggttgccgat gtgctggaca agcgtggcta tcgcaatcag
     2941 gtcgtcagcg gcgcgataca gggactgacg ctcgagacgc gcgtagcagg acccgcctac
     3001 acctgcaagg gcgcgccggc gaccgcgctt gagccggacg attgggacat gcgcaagcag
     3061 ttcctcgaca acgtccggcc cggtaccgtc gtcgttgtcg acaccagtga cgacacagcc
     3121 tcggcgcact ggggtgagtt gatgagcacg gctgctcgcg gccggggcgc aaccggcgtc
     3181 gtcatcgacg gcgccacccg tgacgtgacc cagatgctgg cgatgggctt ccccgccttc
     3241 gtgcgatacc gctcgccggc cagctcgatc cttcgctggc gcatcagtgg ctacgaccat
     3301 cccgtcacga tcggcggtgt cctcgttcgg cccggcgact ttgtcgtcgg cgacgcggac
     3361 ggcgtcgtga tcgtaccggt cgagttggct gtcgaggtgc tggaagaggt cgaatcgctg
     3421 gcggtcgccg agacgaacat gcgccaggaa ttgctggagg gcggcacgtt cagcgacgtt
     3481 ttcgatcggt atcaggttgg ctgacacgac tggttcgcag caacggatgg ttgcggcgct
     3541 cggctacgtc ttcacgccgg tcgtcccgct gatcacgctg acctctgatc tgaagcagga
     3601 tccgttcatg aagcgccacg ccggtcaggc gctgctctgg tcggccggcc tcgtcctcgg
     3661 tttcgtcgtg atcgtcatcg ccgcgatctg gatgctgcgg accgagttgc tgttgatctg
     3721 tctcttcccg gcgctgttcc tgctgccgtt cctgccgggc gcctggtggg gctggcgcgt
     3781 ttatcagggc ggcgatccca ggccgccgct cattgctccg cttgcggcgc ggctttttcc
     3841 ggcacgttag tctgtagtcg cccgagcagg tcaagcgcca gcagcagcgt catcgccgtc
     3901 tgctcgcggt tgatcgcgtc ctgtgcctcc agcgtatgga tgatcgcgtc gttgaaatgg
     3961 cgctgctggt cgagcgcgat ctgcacctga cggcgaagcc caacatcacg caccagtccc
     4021 agcacccgca gcccaagtga ccgcatcccg gccggtgcgt cgtcacgcgg tcgcgccaac
     4081 tcgcgggcgg catcgatgcg cagctccaga agggcggggg gtggcgcgtg caacgccgcc
     4141 ggcgcatccc agccttcacg gttctcgtag ccccgcagca acgactcgac cgcagccgcg
     4201 ccgctgcggg ccggccagcc gtcctcgatc agcgtgtgta actcatggcg cagccgcgcg
     4261 tactcggccg ggacgaagct ggtgcgccac tgcctcccgc tcaggtgctt caaggcgaag
     4321 cgaatgcggt tgcggtgcag atgctggtga taggcgtcgc tccgttcgag cgtcaccccc
     4381 tcgtggtgaa ccgcccgcag cgacggcaaa aagcgcacgc gccaacctgt cgcccgcagc
     4441 cgaacgcaga gatcgacgtc ttcgtagtag acgggcgaga atacgtcgtc gaatccgccg
     4501 atctcctgaa aggcgctcat tcgtaacccc atcgccccac cggtgacgaa gtcgacgtcc
     4561 gtctcggcta gttgcgtctc gccgatccgc tgttcgtaag cgagatgggc ggtcgccagc
     4621 agcggctgtt cgatgacgcc gccggcgtgc tgaatgcggt cggagtcggg atagcgcagc
     4681 agcgggccgg ccgcgccgag cttctcgtca gcggcaaact cggccagcag ggcgctgccg
     4741 aagtcggggt cgagttctac atccggattc atcagcagcg cgaacggagt ttcgacggca
     4801 gtcagtccga cgttggcgcc gccagcgaat cccaggttgg ccggctgggc gaggacgtgg
     4861 acgcggggga agctgtgcag ttcctgaagc gagtcgtctg tcgaggcgtt gtcgaccacg
     4921 acgatctcga ccggcgccgg ttgcgtctgt tccagcgcgc gcaggcaagc atccagatag
     4981 cccgcgccgt tatagttgac gacgactgcc gtccagccgg gaattgacat cctgccccct
     5041 cacccgtgct ccacgacgcg caatgatagc cgctggcgac cgtgagaggc gcgagcatgg
     5101 acaaccttcg accgagcgtg ctgctgatcg cctccgacac gatcggcgag cgaatggccg
     5161 gttcgggcat ccgctactgg aacctggccc gcgtcatcgg cgcccggcag ccggtcacgc
     5221 tcgcgacacc gaacgagacg acactgcccg cgccggccgg cgtgacgctc gtcccctatc
     5281 tgggcagttc cgacgagcgc gggcggcaac tggctgaact ggttggcgcg cacgtggtcg
     5341 tcgtcgcgca gcacttgccg tacctgtaca ccgacgctga cgtcctcgcc tcgcgggcgc
     5401 tggtggtcga tctctacgcg ccgtggatcc ttgagaagct ggagtactcg cgggtcgacc
     5461 ccgagcgggg cgagatcgat cgcaaggacg atgtcacgat cctcaaccgg ctgctggcgc
     5521 tcggtgattt cttcatctgc gcgtcggagc ggcagcgcga cttctggctc ggcgcgctgg
     5581 cggcggccgg ccggctcgat ctgacgcatg cccaggctgg gcccgatcta cggacgctga
     5641 tcgacgtcgt cccattcggc ctgccgcccg acaagccggt tcggaacgga accggcccgc
     5701 gcggtacgtt cgacgggatt ggcgacaacg acatcgtcgc gctctggaac ggcggcctct
     5761 ggaactggct cgacccactg ccggcgatcc gcgcgacggc gcaggtggcc gccgccgagc
     5821 cgcgcttcag gctggtcttc atggggacgc aaagccccgg cgcgcaagtt gccgagatgg
     5881 aaatcgtatc gacggcacgc gaccttgcca g
//
LOCUS       DASUGJ010000114         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_292796, whole genome shotgun sequence.
ACCESSION   DASUGJ010000114 DASUGJ010000000
VERSION     DASUGJ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292796"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFV93_02595"
     CDS             complement(<1..585)
                     /locus_tag="VFV93_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5164260.1"
                     /translation="MLTRLTERVSFAPGGVNIGVIMADDDRAILIDTGLNDSSVRKVL
                     RELDEQGRRVSDIITTHGHADHFGGNAFTVKRTGARVHAPSWDETLLRYPLFQPLSLF
                     AGADPPDSMRTSFLLAKESPVDVIYDAGGMDIAGVEIEAVSLAGHSGNQMGLLVDGVF
                     FCADVILPERVIERYKMPYLYSVRDHLPSLDRAET"
     gene            646..721
                     /locus_tag="VFV93_02600"
     tRNA            646..721
                     /locus_tag="VFV93_02600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:679..681,aa:Arg,seq:ccg)
     gene            1018..1560
                     /locus_tag="VFV93_02605"
     CDS             1018..1560
                     /locus_tag="VFV93_02605"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5164261.1"
                     /translation="MVFRKESRADSFQRQISALRQQLGTDQEDDEFFDDAEPMPDAPA
                     PVDEGTPVAARPPSSPSPRPSQPRVADASTGVIAADSVWSGTLRAQGSLQIFGEVEGE
                     LAANNEIFVAEGARVNARIAAQVIIVAGTVDGTLECSGRLEVLPSGRVSGEVFAPTLV
                     VHEGATVDGDLKMQVAESAG"
     gene            1604..2152
                     /locus_tag="VFV93_02610"
     CDS             1604..2152
                     /locus_tag="VFV93_02610"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5164262.1"
                     /translation="MSFTSTAGSATLDRSTTGMTIIDANTVIDGTLSTSHDLRIDGHV
                     TGTVMCDGVLHVTEGAEVDATVEAAGIVVAGSLSGAITCHGRLEIRSTGRVRGRVETG
                     RLIVVEGAVYEGQLRMDAEQIEPTPPTPLPTQVVEAEPEAAAQGQATGSYSFLRSFTP
                     GSTPARQDDDDLPASPGDDDPE"
BASE COUNT          460 a          766 c          760 g          414 t
ORIGIN      
        1 cgtctcggcc cgatcgagtg acggcaggtg atcgcgaacc gagtacaggt acggcatctt
       61 gtagcgctcg atgacccgct ccggcaggat cacgtccgcg cagaagaaga cgccgtcgac
      121 cagcaaaccc atctgattgc cggagtgccc agccagcgac accgcctcaa tctcgactcc
      181 cgcgatgtcc atcccacccg cgtcgtagat gacgtccact ggcgactcct tcgccagcag
      241 gaagctggtc cgcatcgaat ccggtggatc agcgccggcg aacaacgaga gcggctggaa
      301 cagcggatag cgcaggagcg tctcgtccca gctgggtgcg tgaacacgcg ctccggtccg
      361 tttgacggtg aatgcgttgc cgccgaaatg gtcggcatgg ccgtgggtgg tgatgatgtc
      421 gctgacgcgt ctgccctgct cgtcgagctc gcgtaagacc ttgcggaccg acgaatcgtt
      481 gagcccggtg tcgatcagga tcgctcgatc gtcatccgcc atgatgacgc cgatgttcac
      541 gccaccgggg gcgaacgaca cacgttctgt caatctggtc agcattggag tcctttcgat
      601 caccacgctc ctgaggtata ctacaggggc gattttcgca accgcgcgcc cgtagctcag
      661 tggatagagc gcttgcctcc ggagcaagag gccacaggtt cgagtcctgt cgggcgtacc
      721 accaccttca tcgtcgccga tccgatcagc caggtgcgct cgaacaccgg ccgagaacct
      781 tgaagacaca tccccgattc gatgccggac atacgctcaa agaccgccgc cccgttgtca
      841 ttctgtcaac tctagacacg cctgtacgta cactctatac tcgtggcgac ggatcgcgga
      901 atgcctgcct ggggcgacgc gacgacagac ctgcccgtag atgacgtaac cgattcctca
      961 gttcgcaggt tgatgcagac agcgaccggg ctccgggaca gcgcagggag ttgacagatg
     1021 gtgtttcgca aggagagcag agccgactcg ttccagcggc agatttcggc gctccgccag
     1081 cagctcggga ccgatcagga agacgacgag ttcttcgacg acgccgaacc aatgcccgat
     1141 gcaccggcac cggtcgacga gggtactccc gtcgcagccc ggcctccatc gtcgccaagc
     1201 ccgcgtccca gccagccacg ggtcgcagac gcctcgacgg gcgtcatcgc cgccgattcg
     1261 gtctggagcg ggacactccg cgcacagggg tcattgcaga tcttcggcga agtcgaaggg
     1321 gaactggcag ccaacaacga gatcttcgtt gccgaaggtg cgcgcgtcaa cgccagaatc
     1381 gccgcacagg ttatcatcgt cgcaggcaca gtcgatggca cactcgaatg cagcggccgt
     1441 ctcgaagtgc ttccgagtgg gcgcgtcagc ggtgaagtgt tcgcgccgac gctcgtcgtc
     1501 cacgagggcg cgaccgtcga tggcgatctc aagatgcagg ttgctgaatc ggccggctga
     1561 cggtgcctct ctcagatcgg gctgtatcaa ggagtgacgg cgaatgagct ttacgagcac
     1621 ggcaggttcc gcgacgctgg acagatcgac gaccggcatg acgatcatcg acgccaacac
     1681 cgtcatcgac ggaacgttgt cgacctcaca cgatctccgg attgatggcc atgtcaccgg
     1741 aaccgtcatg tgtgacgggg tcttgcacgt caccgagggt gccgaggttg acgcgaccgt
     1801 cgaagcggcc ggcatcgtcg tcgccggctc gttgagtggc gccatcacct gtcacggccg
     1861 gctcgagatt cgctcgaccg gcagggtgcg aggtcgagtt gagactggcc ggctcatcgt
     1921 tgtcgagggc gccgtctacg aaggccagtt gcggatggac gccgagcaaa tcgagccaac
     1981 tccgccgacg ccgctgccaa cccaggtggt cgaagcggag ccggaagccg ccgcccaggg
     2041 gcaggcgacc ggatcctact cgttcctgcg cagtttcacg cccggttcga cgccggcgcg
     2101 ccaggacgac gatgacctcc cggcgtcccc cggcgatgat gaccccgaat agccgctgac
     2161 gccacaccga gccgtgttat actgcacccc aaccttgatc tgaccaacag atcgttgatg
     2221 tctctcgttc cgtactggtc tgttggcgct gggcatccgc gaggggaagc ggactgcccg
     2281 tgtggcgtgg tatccggcgc gatggggggc gagggcgatg acagggcgac agtgcggctc
     2341 gtgtaagcac tacgaacccg cgccaatctg gcgcaagggt tggtgccgaa acccgctatt
//
LOCUS       DASUGJ010000115         4046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1682788, whole genome shotgun sequence.
ACCESSION   DASUGJ010000115 DASUGJ010000000
VERSION     DASUGJ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682788"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..475
                     /locus_tag="VFV93_02615"
     CDS             170..475
                     /locus_tag="VFV93_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164263.1"
                     /translation="MKYRTVSGDERDRLLGSRKPSGFNYGPYLEVLDNASDGDVVAVE
                     VAQGGERGEKIRFSRAARQRGKSLNWLTSNNDNEIAFQIAGTKPQRPRGRKAQPVAE"
     gene            complement(548..1552)
                     /locus_tag="VFV93_02620"
     CDS             complement(548..1552)
                     /locus_tag="VFV93_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5164264.1"
                     /translation="MSASGKHQREGAQLRVIEGGGEPPRRPLPRVTTQEREHGIRFVL
                     LVVLALNLAVALAKIGFGLFSGSLAITADGFHSMLDALANVVALVGIVVAARPPDPNH
                     AYGHHRYETLTSLGIAGLMLLALFGLVQGAWSRLQSGSAPDVSSLSFIVMGVTLAVNV
                     GVTLWERREARRLSSTLLMADARHTTSDILVSISVIVSLVVVWLGYGWADAGITLAIA
                     AAIAWGAWSIVRDASLVLTDAVVAEAGQIAEAVRSVPGVQGTHNIRTRGAEGYVWVDL
                     HVQVDPGLPVTDAHDIASAVARRVEAELGNAADVTVHIEPADEQHLRDQRGHDPLGMG
                     "
     gene            complement(1701..2528)
                     /locus_tag="VFV93_02625"
     CDS             complement(1701..2528)
                     /locus_tag="VFV93_02625"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5164265.1"
                     /translation="MADGTNDTIDARDEQLELYILGLLDERETAAIERVLRDDPAARQ
                     RARELREVVSVLALDVDPVEPSSGLKARILDAARADLTPDQVAPQPPISLAERRERGV
                     ARWTPWLVAAALALALAASLVWNAQLRSDLDSQPETAIYAVEGSGPAAGVAGSLIVVG
                     DDDSVLTLAGLAALPSDRAYQVWLIADGAPVPNVTFVPNAAGVATVTVPGRIADYHTL
                     AITVEPQTGSLAPTTDPIITSDLTVVVGVSGAGIRDSGLGDVAHRIRGHVMFVDDGM"
     gene            complement(2521..3117)
                     /locus_tag="VFV93_02630"
     CDS             complement(2521..3117)
                     /locus_tag="VFV93_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5164266.1"
                     /translation="MTQPPNDEGHSDGDLLARIAARDDAALGDLYDRYSGLAFGLAYR
                     VLRDRGQAEDVVQEAFLAVWRRAATFDAARGSARGWLTSIVHNAAIDRRRGRFRHQQD
                     EVDIEDHAWRLADDDVWEDVSRRLDREQVRRALAQIPNEQRETLELAYFGGLTQAEIA
                     AQTGEPLGTVKSRARLGLRRLEGLLRGSGTGDESGSGG"
     gene            complement(3248..>4046)
                     /locus_tag="VFV93_02635"
     CDS             complement(3248..>4046)
                     /locus_tag="VFV93_02635"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5164267.1"
                     /translation="RKSHHRSRLGESIMAGHSKWAQIKRDKASNDSKRGAMFSKLARE
                     VYVATREGGPSPDGNVRLRIALERAKKEGMPSDTVERAIAKASGVAGEGEDYQTVFYE
                     GYGPAGVAVMAVALTDNRNRTAAEVRQAFNRHGGSLGETGTVAWQFDTVGQIVIAADG
                     TDPDEITLQAIDAGATDVAAENGEILITTEPGDLSSVTETLREQGIEIGDAAVTRVPQ
                     NTIDIGGNQAETALKLLEALEDLDDVQEVFTNASFEAMSDQLSSVGN"
BASE COUNT          650 a         1355 c         1382 g          659 t
ORIGIN      
        1 gatcataacc agacacgcga tcgcggggat acatgctggg aactattgcc cgatgcaacg
       61 atctcgatat actattgcaa catgcgtcgt tccggcgtaa cgaattaccg gccgttgtca
      121 gcgcaattgt gacgcgatcc ggaatcaaac cagggagggt gtaagcagaa tgaagtacag
      181 gacggtttct ggcgacgaac gcgatcggct gttggggagt cgcaagcctt cgggattcaa
      241 ctatggtcca tatctcgaag tgctcgacaa tgcatccgat ggcgatgttg tcgcggtcga
      301 ggttgcccag ggtggcgagc ggggcgagaa gatccgcttc agccgtgccg cacgccagcg
      361 tggcaagtcg ctcaactggc tgacatccaa caacgacaac gagatcgcat tccagatcgc
      421 cggcacgaag ccgcagcgcc cgcgcggccg caaggcccag ccggtcgcgg agtaacgccc
      481 gcgcgccgat ccaccccggc gtaggggcaa tggccgcagc ctcgtccttt cctgcctggt
      541 tgggcgatta ccccatcccg agcgggtcgt ggccgcgctg gtcgcgcagg tgctgttcgt
      601 cggcgggttc gatatggacc gtgacgtcgg ccgcgttgcc aagctcggcc tcgacccgcc
      661 gggcgacggc gctggcgatg tcgtgggcgt cggtgaccgg cagcccggga tcgacctgca
      721 cgtgcagatc cacccagaca tagccctcgg cgccgcgtgt gcggatgttg tgcgtcccct
      781 gcacacctgg aaccgagcga acagcctcgg cgatctggcc ggcctcggca acgacggcgt
      841 cggtcagcac cagtgaggcg tcgcggacga tcgaccaggc gccccaggcg atggccgccg
      901 cgatcgccag cgtgatcccg gcatccgccc agccatagcc gagccagacg acaaccagcg
      961 agacgataac cgagatcgag acgaggatgt cgctggtcgt gtggcgcgcg tcggccatca
     1021 gcagtgtgct ggaaaggcgg cgcgcctccc gccgttccca aagcgtgacg ccgacattga
     1081 cggccaacgt cacacccatc acgatgaagc taaggctcga gacgtccggc gcgctgccag
     1141 attgcaggcg cgaccaggcg ccctggacaa ggccaaagag cgcaagcaac atcaggccgg
     1201 cgatccccag ggacgtcagc gtctcgtagc gatggtggcc gtaggcgtgg ttcgggtccg
     1261 gcgggcgggc cgcgacgacg atcccgacca gcgcgacgac gtttgccagc gcgtcgagca
     1321 tcgagtggaa gccgtcggcc gtgatcgcca gcgagccgga gaagaggccg aagccgatct
     1381 tcgccagcgc gacggccagg ttgagcgcca gcacgaccag caggacgaag cggatgccat
     1441 gctcgcgctc ctgggtcgtg acgcgcggca gcggccgacg cggcggctct ccgccaccct
     1501 cgatcacccg cagttgcgcg ccttcacgct ggtgcttgcc gctcgccgac acccgagtcc
     1561 cgaccttcca gtcagcgttg tgctgatcgt gtcacacccc cagtctacgg agtcgcgctc
     1621 aggaagtcac agggcaattt gccgatttgg acgggctaaa tcggggtttc gggagccggg
     1681 attcgggatt cggggctggg ttacatacca tcgtccacga acatgacatg tccccgtatt
     1741 cgatgagcca cgtcacccag ccccgaatcc cgaatcccgg ctcccgaaac cccgacgacc
     1801 accgtcaaat cactcgtgat gatcgggtcg gtggtggggg cgagggagcc ggtctggggc
     1861 tcgacggtga tcgcgagggt gtgatagtcg gcgatgcggc cggggacggt gacggtcgcg
     1921 acgccggcgg cgttcggcac gaaggtgacg ttggggaccg gcgcgccgtc ggcgatcagc
     1981 cagacctggt aggcgcggtc ggacggcaac gcggccaggc cggccagggt gaggacgctg
     2041 tcgtcatcgc cgacgacgat cagcgagccg gccacaccgg cggcggggcc gctgccttcg
     2101 acggcataga tcgccgtctc gggctgggag tcgaggtcgg agcgcagctg cgcgttccag
     2161 acgaggctgg ccgccagcgc cagcgccagc gccgccgcga ccagccacgg cgtccagcgc
     2221 gcgacacccc gctcgcgtcg ttcggccagc gagattggcg gttgcggcgc gacctgatcg
     2281 ggcgtcaggt cggcgcgagc ggcgtccagg atgcgcgcct tgaggccgga ggatggctcg
     2341 accggatcga catccaaggc caggaccgag acgacctcac gcagctcgcg ggcgcgctgg
     2401 cgggcagccg ggtcgtcgcg cagcacgcgc tcgatggcgg ctgtctcacg ctcgtcaagc
     2461 aggccgagga tgtacagctc gagctgctcg tcgcgggcgt cgattgtgtc gttcgtgccg
     2521 tcagccacct gatccgcttt catcacccgt gccgctgccg cgcagcaatc cttcgagccg
     2581 tcgcagcccc agccgggcgc ggctcttgac ggttccgagc ggctcgccgg tctgggcggc
     2641 gatctcggcc tgggtcagcc cgccgaagta ggccagctcc agtgtctcgc gctgctcgtt
     2701 ggggatctgc gccagcgccc ggcgcacctg ctcgcggtca agcctgcgtg agacgtcttc
     2761 ccagacgtcg tcatcggcca gccgccaggc atggtcctcg atatcgactt cgtcctgctg
     2821 gtgccggaag cggccccgcc gccggtcgat cgcggcgttg tggacgatcg aggtcagcca
     2881 gccgcgcgcg cttccccggg cggcgtcgaa ggtcgctgcc cggcgccaga cggcgagaaa
     2941 cgcttcctgc acgacatcct ctgcctgacc gcgatcgcgc agcacacgat aggccagccc
     3001 gaacgcgagg ccgctgtagc gatcgtagag atcgccgagc gccgcgtcat cacgcgcggc
     3061 gatccgagcc agcagatcgc cgtcgctgtg gccctcgtcg ttcggcggct gcgtcaccgg
     3121 cgctcctcac gcgcgttcaa cagactgtac gcttcgtgct catgagtgga tgactggccg
     3181 tcgggccggg atcacgaaag gcgacccgtt gcgctgacgc aacgagccgc cggctggatg
     3241 tcgcgcgcta gttgccgacc gacgagagct gatcgctcat cgcttcgaag gatgcgttgg
     3301 tgaagacctc ctgaacgtca tcgaggtctt cgagagcttc gagcagcttg agcgccgtct
     3361 cggcctggtt gccgccgatg tcgatcgtgt tctgcgggac acgggtgacg gccgcgtcgc
     3421 cgatctcgat cccctgctcg cgcagcgtct cggtcacgct gctgagatcg cccggctcgg
     3481 tcgtgatcag gatctcgccg ttctcggcgg ccacgtcggt cgcgccggcg tcgattgcct
     3541 gcagcgtgat ctcgtccggg tcggtgccgt cggccgcgat gacgatctgg ccaacggtgt
     3601 cgaactgcca ggcgacggtg ccggtctcac ccagcgagcc gccgtgccgg ttgaacgcct
     3661 ggcggacttc ggcggcagtc cggttgcggt tgtcggtcag tgcgacagcc atgacagcca
     3721 cgccggccgg gccgtagccc tcgtagaaga cggtctggta gtcctcgccc tcgccggcga
     3781 cgccgctggc cttggcgatc gcacgctcga ccgtgtcgct cggcatgccc tctttcttgg
     3841 cacgttcgag cgcgatccgc agccggacat tgccgtccgg gcttggaccg ccctcgcggg
     3901 tggcaacata gacctcacgg gcgagcttgg agaacatcgc tccccgcttc gaatcgttgg
     3961 acgccttatc gcgcttgatc tgtgcccact tggaatgtcc ggccataatg ctctccccca
     4021 acctggatcg gtgatgcgat ttgcgg
//
LOCUS       DASUGJ010000116         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1961247, whole genome shotgun sequence.
ACCESSION   DASUGJ010000116 DASUGJ010000000
VERSION     DASUGJ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961247"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VFV93_02640"
     CDS             complement(<1..659)
                     /locus_tag="VFV93_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5164268.1"
                     /translation="MNVDLVIRGGTVVNAASRCQADIGILGERIVQIGGDLDGTREID
                     ARGLLVLPGAIDAHVHLTSDEPDPDAVQWADDFTSGSEAALAGGVTTVGNMTFLRSGE
                     AMLEGLAREERLVAEQAIADVILHPVIGDPRQAIIDEVDELLDAGHADIKYFMSTPTF
                     DANALGFLKATDAAGRNGLLTMIHCEDYPMIRWSVDRLLAEGRGGLENYPASRPVVSE
                     AV"
     gene            802..1974
                     /locus_tag="VFV93_02645"
     CDS             802..1974
                     /locus_tag="VFV93_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5164269.1"
                     /translation="MTVTSQIDTAALRASEFPVTGIWSYLNHATTGPLPQRTIRALDE
                     INRAFAVPHIWEAGDRGAPLCAVREGIARIAGGQPERVAIVASAAHGLSICAAGVDWR
                     DGDEVVIPQSEYPSLAMPFLAQADRGLRVRWAAKSADGRTDLNAIEAAITPRTRAIAL
                     SHVEFADGHRNDLAALGTLCRDRGLLLFVDATQSLGAVPIDVDGWGIHAAVAHGYKWL
                     HAGFGIGVALFSAEGMERVTPTHGGSASVCENPYVAEPRLSWHPTAQRFEPGGQPFTM
                     ISGLQASLSLLEEVGTDQILPHAITLLDQLVAGVTAKGYTIASSLEPAHRSQFLALTS
                     GSADGDLRAHVALSEAGVVTVIRSRGIRVAPSFYNDADDIERLIDALPPLPDSTPH"
     gene            2014..>2254
                     /locus_tag="VFV93_02650"
     CDS             2014..>2254
                     /locus_tag="VFV93_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5164270.1"
                     /translation="MRTTSPLKPTIDDVVTARERLRGIAIRTPLLQLHGSSTVWIKPE
                     CLQPVGSFKMRGIYNAVAALPDDERAGGVSTVSSGN"
BASE COUNT          378 a          745 c          746 g          385 t
ORIGIN      
        1 acagcttccg agacgaccgg ccggctggcg ggatagttct ccagcccgcc gcgtccttcg
       61 gccagcagcc ggtcgaccga ccagcggatc atcgggtagt cctcgcagtg gatcatcgta
      121 agcaggccgt tgcgaccggc cgcgtcggtt gccttgagga agccgagcgc gttggcatcg
      181 aacgtcggtg tcgacatgaa gtacttgatg tcggcatggc cggcgtcgag cagctcatcg
      241 acctcgtcga tgatcgcctg gcgcgggtcg ccgatgaccg gatgcaggat gacatcggca
      301 atggcctgct cggccaccag ccgttcctcg cgggccagcc cttcgagcat cgcctcaccg
      361 gatctgagga aggtcatgtt gccgactgtc gtcacgccac cggccagcgc cgcctcggag
      421 ccgctcgtga aatcgtcggc ccactgaacg gcgtcagggt cgggttcgtc actggtgagg
      481 tggacgtggg cgtcgatcgc gcccggcagg accaacagcc cgcgcgcgtc gatctcgcgc
      541 gtcccgtcga gatcaccgcc gatctggacg atccgctcgc cgagaatccc gatgtcggcc
      601 tgacaacgcg acgcggcgtt gacaaccgta ccgccacgga tgaccagatc aacgttcatt
      661 gccagcatcc tccccggaat cgcgcgacct gccgctggca gcatgctact tgttcatggc
      721 gatcccgtca ctgcctgttg cggcaagcgg ggtcgttggc cgacaatggc tgcctgcctg
      781 atgcgtgaac ggaggcggca aatgaccgtg accagccaga tcgacacggc ggccctgcgt
      841 gccagcgaat ttccggtgac gggcatctgg tcctacctga accacgcgac gaccgggccg
      901 cttccgcagc gaacgatacg cgcgctcgac gagatcaacc gggccttcgc cgtcccacat
      961 atctgggagg caggcgatcg cggcgctccg ctctgcgccg tccgtgaggg aatcgcgcgc
     1021 atcgccggcg gccagcccga gcgcgtcgcg atcgtcgcgt cggcagcgca cgggctcagc
     1081 atctgcgcgg ccggagttga ctggcgtgat ggggatgagg tcgtcatccc gcagtccgag
     1141 tacccgtcgc tggcgatgcc gttcctcgcc caggcggacc gtggcctgcg ggttcggtgg
     1201 gcagcgaagt ccgccgacgg ccggaccgat ctgaacgcaa ttgaggctgc catcacgccc
     1261 cgaacgcgcg cgattgcgct ctcgcacgtc gagttcgccg acggacaccg caacgacctg
     1321 gcggcgctag gcacgctctg ccgcgatcgc gggctgctgt tgttcgttga tgcgacccag
     1381 tcgctcggtg cggtcccgat cgatgtcgat ggttggggca tccacgccgc cgtcgcgcac
     1441 ggctacaagt ggctgcatgc cggattcggc atcggcgtcg ccctcttctc agccgagggg
     1501 atggagcgcg tcacgccaac gcatggcggc agtgcctcgg tttgcgagaa cccgtacgtc
     1561 gcggagccgc gtctgagctg gcacccgacc gctcagcgct tcgagccggg tgggcagcca
     1621 ttcacgatga tcagcggcct gcaggcgtca ttgtcgctgc tggaggaggt tgggacagat
     1681 cagattctgc cgcacgcgat cacgttgctg gatcaactgg tggccggcgt caccgcgaag
     1741 ggctacacga tcgccagcag tctcgaaccg gcccatcgct cgcagttcct ggcgctgacg
     1801 agcggatcgg cagacggcga tcttcgcgcg cacgtcgcgc tgagcgaggc cggcgtcgtg
     1861 acggtcatcc ggtcgcgtgg gatcagggtt gcgccgagct tttacaatga cgccgacgat
     1921 atcgaacgat tgattgacgc cttgcctccg ctaccagact caaccccgca ttgagagggc
     1981 gagtcaccag tcacacgcac aaagggaatc accatgcgta ccacctcccc gctcaagccg
     2041 acgatagacg atgttgtgac ggctcgggag cggctacggg gaatcgcgat ccggacaccg
     2101 ctgctgcaat tgcacgggtc atcgaccgtc tggatcaagc cggaatgtct tcagccggtc
     2161 ggcagcttca agatgcgcgg gatctacaac gcggtggcgg cgctaccgga cgatgaacgc
     2221 gctggtggtg tcagtactgt ctcgtccggc aaca
//
LOCUS       DASUGJ010000117         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1404577, whole genome shotgun sequence.
ACCESSION   DASUGJ010000117 DASUGJ010000000
VERSION     DASUGJ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404577"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2276
                     /locus_tag="VFV93_02655"
     CDS             <1..>2276
                     /locus_tag="VFV93_02655"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX5164271.1"
                     /translation="SVLAETGQRSTQVDIRVERYVLPKYEVRIELERDWALVNEPISG
                     LIAAEYAFGKPVNGEVELVASRYVGAWEKYATVRQPISDGRLVFEVPPVEYVTGSPDG
                     GGLGSVQLEAKVIEQATGYEETTTQLVSIAQGPVVLQVIPDSSSFKPGLQLTLLVVTE
                     SPGRQPVDARVYLNLSWQDDEYGDAGFDNETVETRNGVATLTITPPDDAAILTVDASV
                     DEMTSTYLTLRAGYSPSNTFIHVTQETSGALAVGQTARFQIAATNEARSFFYEVLARG
                     KVVFSAVVTSPEIELTLTPMMAPEARLLVYQILPGGEVAADYLPFSVSGDYPLAVSVD
                     PGVEEAAPGDALAIAVQTDGPARVGLVAVDRSVFILAENRLNLQQVFAELERLSLQPQ
                     AELHEAEGGIEVQPMFDPWGPTTIPGAEETFRDAGVIVLTNQRVPRGKEIQPDMFRFA
                     AGASEDDDDAAAPVPMAATAANVEEAGRAVQTQAQPQRIRQFFPETWIWSDLTTDASG
                     HASQPATAPDSITTWVFRAVALSRELGLGIGETELRVFQSFFVSVDLPFAAIRGEELP
                     AAVALYNYTQAAEEFTVELEPADWFEPTGSLTQTVTVGPNEVGAASFPIRTTALGVHP
                     LRVTARGATVADAIVKEMIVEPEGVAREMLENLVLASGSVREIDLSLPDFTVDGSGRA
                     FLALTGNVLSQTMDGLESLLQMSFGCGEQNMILFAPNVFITRYLDETGQLKPEVRARA
                     ETLMLTGYQREMTYRRSDG"
BASE COUNT          381 a          776 c          758 g          361 t
ORIGIN      
        1 tcggtcctgg ctgagaccgg ccagcgtagc acccaggttg atatccgcgt cgagcgctat
       61 gtcctgccga agtacgaggt gcggatcgag ctggagcgcg actgggcgct cgtcaacgag
      121 ccgatcagcg gcctgatcgc cgccgagtac gcctttggca agccggtcaa cggtgaggtc
      181 gagcttgtcg cctcgcgcta cgtcggcgcc tgggagaagt acgcgaccgt tcgccagccg
      241 atcagcgatg gccggctcgt cttcgaggtg ccgccggtcg agtatgtcac cggatcgccg
      301 gacggcggcg gtctcggcag cgtccagctt gaggccaaag tcatcgagca ggcgaccggc
      361 tatgaggaga cgacgacgca actggtgtca atcgcgcagg ggccggtcgt cttgcaggtc
      421 atccccgata gcagcagctt caagccaggg ctgcagctga cgctgctggt cgtcaccgag
      481 tcgccgggcc ggcagccggt cgatgcgcgc gtctacctca atctcagctg gcaggacgac
      541 gagtacggcg acgccggctt cgacaacgag accgtcgaaa cccgcaatgg agtcgcgacg
      601 ttgaccatca cgccgccgga tgacgccgcg atcctgacgg tggatgcctc ggtcgatgaa
      661 atgacctcta cctatctcac gctgcgggcg ggctactcgc ccagcaacac gttcatccac
      721 gtgacgcagg agacgagcgg ggcgctggcg gtcggccaga cggcgcgctt ccagattgcc
      781 gccacgaacg aggcgcgcag cttcttctat gaggtgctgg cgcgcggcaa ggtcgtcttc
      841 tcggctgtcg tcacctcgcc ggagatcgag ctgacgctga cgccaatgat ggcgcccgaa
      901 gcgcggctgc tggtctacca gatcctgccc ggcggcgagg tcgctgccga ctacctgccg
      961 ttctccgtta gcggcgacta cccgctggcc gtcagcgtcg atccaggagt ggaggaagca
     1021 gcgccaggtg atgcgctggc gatcgccgtc cagactgacg ggccggcgcg ggttgggctt
     1081 gtcgccgtcg atcgatccgt cttcatcctg gccgagaacc ggctcaacct gcagcaggtc
     1141 ttcgccgagt tggaacggct ctcgctccag ccacaggcag agctccacga ggccgaaggc
     1201 gggatcgagg tccagccgat gttcgacccg tggggaccga cgacgatccc cggcgctgaa
     1261 gagaccttcc gcgacgccgg cgtcatcgtc ctgaccaacc agcgggtgcc gaggggcaag
     1321 gagatccagc cggacatgtt ccggttcgca gccggcgctt ccgaggacga tgacgatgcc
     1381 gccgctccgg tgccgatggc agccacagcc gccaatgtcg aggaagccgg cagggccgtc
     1441 cagacccagg cccagccaca gcgcatccgc cagttcttcc ccgagacgtg gatctggtcc
     1501 gacctgacga ccgacgcatc cggccacgcc agccaaccgg cgaccgcgcc cgacagcatc
     1561 acgacctggg tgttccgggc cgttgccctc tcgcgcgagc tggggctcgg catcggcgag
     1621 accgagctgc gcgtcttcca gtccttcttc gtcagcgtcg acctgccctt cgccgccatc
     1681 cgtggcgagg agctgccggc ggccgtcgcc ctctataact acacccaggc cgccgaggag
     1741 ttcacggtcg agctggagcc ggccgactgg ttcgagccga ctggcagcct gacccagacc
     1801 gttacggtcg gcccaaacga ggtcggcgcg gcctccttcc caatccggac gacggcgctc
     1861 ggtgtccacc cgctgcgggt gacagcccgt ggcgcaacgg tcgccgacgc gatcgtcaag
     1921 gagatgatcg tcgagccgga gggtgtcgcc cgcgagatgc tggagaacct cgtcctggcc
     1981 agcggcagcg ttcgtgagat cgacctgagc ctgcccgact tcaccgtcga cggctcgggc
     2041 cgcgccttcc tggcgctgac cggcaacgtc ctcagccaga cgatggacgg gctggaatcg
     2101 ctgttgcaga tgtcgttcgg ttgcggcgag cagaacatga tcctcttcgc gccaaacgtc
     2161 ttcatcaccc gttacctgga cgagaccggc cagctcaaac cggaggttcg cgcccgggcc
     2221 gagacgctga tgctgaccgg ctaccagcgc gagatgacct accgccgctc ggacgg
//
LOCUS       DASUGJ010000118         4092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_432444, whole genome shotgun sequence.
ACCESSION   DASUGJ010000118 DASUGJ010000000
VERSION     DASUGJ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432444"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="VFV93_02660"
     CDS             <1..1256
                     /locus_tag="VFV93_02660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5164272.1"
                     /translation="QPSWSPDGSQLAAIGNWDPASGGAKNDNLWVVPAGGGEARNLSG
                     DFDRSIGDSAMSDVYAGSSNRPLWAPDGASLLMLVSDSGSTHIYRVSLDGGDITRITG
                     GERRVSGVSLSSDGKTIAYVAGTSTNPGDLYVANIDGSDERQLTNLNQDFLGSLTLSE
                     PEEFRVPSQSDGAEIQGWVMKPVGFEAGKKYPMILEIHGGPHAMYSNHMMHEFQLMAA
                     RGYVVVYCNPRGSAGYGEDFTTYTHKAWGEKDMPDVMAAVDHVVSQGYVDANRLGITG
                     GSYGGYMTLWMIGHTDRFKAAVTQRCVSNLYSFYGTSDIGFTFGEYEFGGTAWEQREH
                     FMKYSPITYVDKVTTPLLIVHSEQDYRCPIEQAEQVFISLKKLGREVEFVRFPDENHN
                     LSRTGKPKHRIERLEFIIGWFDSHL"
     gene            complement(1348..2139)
                     /locus_tag="VFV93_02665"
     CDS             complement(1348..2139)
                     /locus_tag="VFV93_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5164273.1"
                     /translation="MVDVSKAQGRLSDRRMLEEMSWPQVRAALDAGQRTVVAAAGSME
                     QHGPQLPLQTDTLLGTTLVAAIIQRLPGVFQGPTIPFGVSEHHMPFAGTITLDKEAFK
                     TVVRQYAESLASHGFETIIVIPSHGGNFGPLREVEEEIGGRAGNARFITYHDLMAFMD
                     PMNQVAARDGITAAVAGAHAGEAETSLVLAARGDLVAMEYAEEGFVGAFDEKAAAAVF
                     EKGMIALTSNGILGDARPATAERGLAYRDAMADFLAEWIRERVPV"
     gene            complement(2132..3394)
                     /locus_tag="VFV93_02670"
     CDS             complement(2132..3394)
                     /locus_tag="VFV93_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEX5164274.1"
                     /translation="MKLLHFSDLHLGVELYGTLDSRTGLSSRVGDFLKVFDIIVEQAI
                     EGGYDAVLFSGDAFKNRDPSPTLQREFARRVFRLAQAGVPIVIIVGNHDLPNVLTRAT
                     PVEIYQVLSIPGVHVCRTIERIDVETKSGKLQVVALPWVTRSMLLAADQYRAMGDVDL
                     EREMSSLIANAVRGLMAELDPTLPAVLMAHVSVQGASLGYEQSIMLGRDVTVGLDDLN
                     SRGFDYVALGHIHKHQVLGTNPPAVYAGSPERIDFGEEREAKGYVRVTIEPDQGRERT
                     TTWEFVELPARVFKTVRIAAFGDDPAETARRDVVRLADEFRGAVTRCFIEVEPGREKS
                     VNAQEIRRLLLERGAGVVTHVVVESETTGRVKDPISADTARDSIKMLQRWVEMRNYDP
                     ALRGRVVERGRELIERRQLEQGRSGSRG"
     gene            complement(3398..>4092)
                     /locus_tag="VFV93_02675"
     CDS             complement(3398..>4092)
                     /locus_tag="VFV93_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5164275.1"
                     /translation="SVDALKRKLAYLDERPYIRKASSEDPVRDIVQHLRQGRHVIIQF
                     GRYHELRDYMLVTNLLTRRIHSEYTEAALKRDSALGLSSDRPVTVVLEEAHKFLSPAA
                     ARQSIFGTIAREMRKFGVSLMVVDQRPSQIDSEVMSQLATRISGLLTDENDIAAVLSG
                     TGDRSALRSMLASLEPSQQCLVVGHAIPMPMIVRTRAYSRALSDSANGKRPDQKLGLS
                     ILMGGRNDGGTD"
BASE COUNT          711 a         1451 c         1230 g          700 t
ORIGIN      
        1 gccagccgag ctggtcgccc gacggcagcc agctggcggc gatcggcaac tgggacccgg
       61 cgtccggtgg cgccaagaac gacaacctct gggtcgtacc ggccggcggc ggcgaagcgc
      121 gcaacctcag cggcgacttc gaccgctcga tcggcgactc ggccatgagc gatgtctacg
      181 ccggctcgtc caaccgcccg ctctgggcgc cggacggcgc cagcctcctg atgctcgtca
      241 gcgactccgg ctcgacccat atctaccgtg tctcgctcga tggcggcgac atcacccgca
      301 tcaccggtgg cgagcggcgc gtatcgggcg tctcgctctc cagcgatggc aagaccatcg
      361 cctacgttgc cggaacgtcg accaaccccg gagacctcta cgtcgccaac atcgacggca
      421 gcgacgagcg ccagctgacg aacctgaacc aggacttcct cggcagcctc acgctctccg
      481 agccggaaga gttccgcgtc ccgtcacagt cggacggcgc cgagatccag ggctgggtca
      541 tgaagccggt cggcttcgaa gcgggcaaga agtaccccat gatcctcgag attcacggcg
      601 gaccgcacgc catgtactcc aaccacatga tgcacgagtt ccagctgatg gcggcccgtg
      661 gctacgtcgt cgtctactgc aacccgcgtg gctccgccgg ctacggcgaa gacttcacga
      721 cctacaccca taaggcctgg ggcgagaagg acatgccgga cgtgatggca gccgtcgacc
      781 acgtcgtcag ccagggctac gtcgatgcca accgcctcgg catcaccggc ggctcctacg
      841 gcggctacat gacgctctgg atgatcggcc acaccgaccg cttcaaggcg gccgtcaccc
      901 agcgctgcgt ctcaaacctg tactcgttct acggcacgag cgacatcggc ttcacgttcg
      961 gggagtacga gttcggcggg accgcctggg aacagcgcga gcacttcatg aagtactcgc
     1021 cgatcaccta cgtcgacaag gtgacgacgc cccttctaat cgtccactcc gagcaggact
     1081 accgctgccc gatcgagcag gccgagcagg tcttcatctc gctcaagaag ctgggccgcg
     1141 aggtcgagtt cgtccgcttc cccgacgaga accacaacct ctcgcggacc ggcaagccca
     1201 agcaccgcat cgaacgcctg gagttcatca tcggctggtt tgattcgcat ctgtagggcg
     1261 taacgcgtga cgcgtaatgc gtgacgcgag gttaaggaaa atcgtcctcg caacctcgcg
     1321 tcacgcatta cgcgtcacga atcctgccta aaccggcacc cgctcccgaa tccactcggc
     1381 caggaaatcg gccatcgcgt cacggtaggc gaggccgcgt tcggcggtgg ccgggcgggc
     1441 gtcgccgagg atgccgttgc tggtcagcgc gatcatgccc ttctcgaaaa cggcggctgc
     1501 cgccttctca tcgaacgcgc cgacgaagcc ttcctcggcg tactccatcg cgacgaggtc
     1561 gccgcgcgct gccagcacca gcgatgtctc tgcctcaccg gcgtgggcac cggccaccgc
     1621 cgctgtaatc ccgtcgcggg ccgcaacctg attcattggg tccatgaagg ccatcaggtc
     1681 gtggtaggtg atgaagcgcg cgttgccggc cctgccccct atctcctcct ccacctcacg
     1741 caacggcccg aagttgccac cgtggctggg gatgacgatg atcgtctcga agccgtgcga
     1801 tgccaggctc tcggcgtact ggcggacaac tgtcttgaac gcctccttgt cgagtgtgat
     1861 cgtcccggcg aacggcatgt ggtgctcgga gacgccaaac gggatcgtcg gcccctggaa
     1921 cactcctggc agacgctgga tgatcgccgc gacgagcgtc gtgccgagca acgtatcagt
     1981 ctgcagtggc agttgtggcc cgtgctgctc catcgagccg gccgccgcga caacagttcg
     2041 ctggccggcg tcgagcgcgg ccctgacctg cggccacgac atctcctcca gcatccggcg
     2101 gtcgctcagg cgcccctgtg ccttgctgac atcaaccacg actcccgctc cttccctgct
     2161 ccagctggcg gcgctcgatc agctcgcgac cccgttcgac gacgcggcca cgcagcgccg
     2221 gatcgtagtt tcgcatctcc acccagcgtt gcagcatctt gatgctgtcg cgggccgtgt
     2281 cagcgctgat cgggtccttc acgcgccccg tcgtctcgct ctcgacgacc acgtgcgtga
     2341 cgactccggc cccacgctcc agcagcagcc gtctgatctc ctgcgcgttg acgctcttct
     2401 cccgtcccgg ttcgacctca atgaagcagc gcgtcaccgc ccctcgaaac tcgtccgcca
     2461 gtcgtacgac gtcgcggcgg gccgtctcag ccgggtcgtc gccgaaggcc gcgatccgca
     2521 ccgtcttgaa gacccgcgca ggcagctcga caaactccca ggtggtcgtt cgctcccgcc
     2581 cctggtccgg ctcgatcgtg acgcgaacgt agcccttcgc ctcccgctcc tcgccgaagt
     2641 cgatccgctc cgggctaccg gcatagacgg ccggcgggtt cgtcccgagc acctggtgct
     2701 tatggatatg tccaagggcg acgtagtcga acccgcgcga gttgagatcg tccagcccaa
     2761 ccgtcacgtc gcggccgagc atgatcgact gctcgtatcc cagcgacgcg ccctggacgc
     2821 tgacatgcgc catcaggacc gcgggcaggg tcgggtcgag ctctgccatc aggcccctga
     2881 cagcgttcgc aatcagcgac gacatctccc gctcgaggtc gacatcgccc atcgcccggt
     2941 actggtcagc cgcaagcagc atcgagcgtg tcacccacgg cagcgcgacg acctggagct
     3001 tgcctgactt cgtttcgacg tcgatccgct cgatcgtccg gcagacatgc acaccgggaa
     3061 ttgagagcac ttgatagatc tcgaccggcg tcgcgcgggt cagcacattc ggcaggtcat
     3121 ggttgccgac aatgatcacg atcggcactc cagcctgcgc caatcggaac acccgccggg
     3181 caaactcccg ttgcagcgtc gggctagggt cgcggttctt gaaggcatcg cctgagaaga
     3241 ggacggcgtc gtagccgccc tcgatcgcct gctcgacgat gatgtcgaac accttcagaa
     3301 agtcgccaac ccggctcgac agtcccgttc gcgagtcgag cgtcccatac agctcgacgc
     3361 ccaggtggag atccgagaaa tgcagcagtt tcaccggcta gtcagtgccc ccatcgttgc
     3421 gcccgcccat caggatgctg agccccagct tctggtcggg ccgcttgcca ttcgcgctgt
     3481 cgctcagcgc ccgtgagtag gcgcgcgtcc tgacgatcat cggcatcggg atcgcgtggc
     3541 cgacgacgag acactgctgg gacggctcca ggctggccag catcgagcgc agcgccgagc
     3601 gatcacccgt cccgctcagg acggccgcga tgtcgttctc gtcggtcagc agcccgctga
     3661 tgcgcgtcgc cagctgcgac atgacctcgc tgtcgatctg gctgggccgc tggtcaacaa
     3721 ccatcagcga gacgccgaac ttgcgcatct cccgcgcgat cgtgccgaag atcgactgcc
     3781 gcgccgccgc cggactgagg aacttatgcg cctcttccag gacgaccgtc accggccggt
     3841 cgctgctcag accaagcgca gaatcccgtt tgagcgccgc ctcggtgtac tcgctgtgaa
     3901 ttcgccgcgt cagcagattc gtcaccagca tgtagtcacg cagctcgtga tagcgaccaa
     3961 attgaatgat aacgtgccgc ccctgacgca ggtgctggac gatatctcgc accgggtctt
     4021 cgctcgaagc cttgcgaatg tacggtcgct cgtccagata ggcgagcttg cgcttgagcg
     4081 catcgacgga gc
//
LOCUS       DASUGJ010000119         4114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1961769, whole genome shotgun sequence.
ACCESSION   DASUGJ010000119 DASUGJ010000000
VERSION     DASUGJ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961769"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFV93_02680"
     CDS             <1..967
                     /locus_tag="VFV93_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX5164276.1"
                     /translation="VGSELEALIFESQLIRRYRPRYNTVQRNAEQYVYVKVDVSNPWP
                     RVTTCRDRAEDGARYFGPFKSSSRARDAVELVNTVLPLRTCRRSFADKRSLGSPCLEL
                     ELKHCLGPCVGRADPDEYRGLVRDVVAFFEGDTTRMIARLHAKLQETVTTLDFERAAR
                     LRDRIRQVERLAHEQHLLDGAGRYQHALLVLPSLETDERQVWYLLKGRRWAQLVVADT
                     ESPATLAERLEPIAQRARQGAAAFVPDHHAVDELSILARWMRRTPEHPALIGLDDADL
                     RDAARLAMAVDVSVPFGDVSLADERDDEDDAQAGAESEMGIAGGS"
     gene            957..1583
                     /locus_tag="VFV93_02685"
     CDS             957..1583
                     /locus_tag="VFV93_02685"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5164277.1"
                     /translation="MGADDRIRILDEVATLVRSCGRCELCRGRTHAVPGHGNIQTEIM
                     FIGEAPGQHEDRQGLPFIGASGQFLNHLLSTIELSRDDVFITNIVKCRPPGNRDPLPD
                     EIAACGSYLDTQIDAIDPLMIVTLGRFSMSKWFPNERISRIHGQPRTFGKRVVVPMYH
                     PAAALHQASLRSTIEADFERLPRILAQAREQLAPAEEQPDQAAQMRLF"
     gene            1734..3992
                     /locus_tag="VFV93_02690"
     CDS             1734..3992
                     /locus_tag="VFV93_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX5164278.1"
                     /translation="MAARAKAKSTQQGTTRRVSVTAPPKPARPSRIGRLARWVGGWFA
                     SIDIPQRLREELIAFAVFTLAVVAALPLVGLAKGSVLDYLGSATRALFGMAAIVVPLG
                     LSVIAVEVWRAGEAGQRVRRIVGGVVTVASIVGLMTLGQTDVDVAQTGGAAGWAVATG
                     LMRLLGEVGAGLTLIALGFAGVFLVTGYTVREISESWQRVRPRRRYAAAGWELDDAQG
                     ESAIESATEPFQRAATQEMSAATVAPVAEAVAAAPVIKVPKPDRPAPVAKPKDDTPEA
                     EPEQRAAPRGKWKLPSINQLALYENSEPDSSDLEAKAKRIEETLSSFKVEAKVREVFP
                     GPAVTLFTVQPGSGVKVRRITELQNDLALALAAPSIRIEAPVPGMARVGIEIPNASIA
                     TVGLRETLESSGFTKSNAKLPLPLGRDVNGRYIVGDLAKMPHLLIAGSTGSGKSVCLN
                     GIIATFLLSKTPDDLKMIMIDPKMVEMAGYEGVPHLKAPVVTEMNKVVSALRAVLHEM
                     ERRYQLFSRLGVRNIDGYELRRSTEPTLEKMPYMVVIIDELADLMMTTPDEVETILVR
                     LAQMARATGIHLIIATQRPTVDVLTGLIKANVPARIAFAVTSVTDSRVILDVAGAERL
                     LGRGDMLFMPADAAKPYRIQGSYIDDQDLQKIVKHWRLLAPETIYEPEWAQLPADDEA
                     DEDSEDPLMEQALNIVRQQGTASASMLQRRLRIGYNRAARLIEQMEEEGIIGPADGSK
                     VRPVISMDDWDE"
BASE COUNT          724 a         1284 c         1394 g          712 t
ORIGIN      
        1 agttggcagc gagctggagg cattgatctt cgaatcgcag ttgatccgtc gctaccggcc
       61 ccgctacaac accgtccagc gcaacgccga gcagtacgtc tacgtcaagg tggatgtctc
      121 gaatccgtgg ccgcgcgtca cgacctgtcg tgatcgggct gaggatggcg cgcgctactt
      181 cgggccgttc aaatcgtcgt cgcgtgcgcg ggacgcggtc gagctggtca acacggttct
      241 gccgctacgg acctgtcgcc gctcgttcgc cgacaagcgc tcgctggggt cgccgtgcct
      301 tgaactggaa ctgaagcact gtctcggccc ctgtgtcggc cgggcggacc cggatgagta
      361 tcgagggctg gtacgcgacg tcgtggcttt cttcgaaggt gacacgaccc ggatgatcgc
      421 caggttgcac gccaagttgc aggagacggt gacgacgctc gatttcgagc gcgcggccag
      481 gctgcgcgac cgcatccgcc aggtcgagcg gctggcccac gaacagcacc tgcttgacgg
      541 cgccggccgc taccagcacg ccctgctggt tctgccgagt ctagagaccg acgaacgaca
      601 ggtctggtat ctgttgaagg ggcgacgttg ggcgcaattg gtggttgccg acactgaatc
      661 accggccaca ctggccgaac gacttgagcc gatcgcccaa cgcgcgcggc agggagcagc
      721 cgcgttcgta cccgaccatc acgcagttga cgagctgtcg atccttgcgc gctggatgcg
      781 ccgaacgcct gagcatccgg cgttgatcgg ccttgacgac gccgatcttc gcgacgcggc
      841 gcgactcgcg atggcggttg acgtctcggt cccgttcggc gatgtgtcgc tggccgacga
      901 gcgggatgac gaagacgatg cgcaggcagg cgcggagagt gagatgggga tagccggtgg
      961 gagctgacga caggattcgg attctcgacg aggtcgcgac gctggtacgt agctgcggac
     1021 gctgcgaact ctgccgcggg cgaacgcacg cggtgccggg ccacggtaac atccagacgg
     1081 agatcatgtt catcggcgaa gcgcccggcc agcacgagga ccgccagggt ctgccgttca
     1141 tcggcgcgtc cgggcagttt ctcaaccacc tgctgtcgac gatcgagctg agccgcgacg
     1201 atgtcttcat caccaacatc gtcaagtgcc gcccaccggg taaccgcgac ccgctgcccg
     1261 acgaaatcgc ggcctgcggc tcgtacctcg atacgcagat cgatgcgatc gacccgctga
     1321 tgatcgtaac gctgggccgc ttctcgatgt cgaagtggtt tcccaacgaa cgtatctcgc
     1381 gcatccacgg ccagccacgc accttcggca agcgggtcgt cgtcccgatg taccatccgg
     1441 ccgcagcgct ccaccaggcg tcgctacgta gcacaatcga agccgacttc gagcgcctgc
     1501 cacgcatcct tgcccaggct cgcgaacaac tcgccccggc cgaggagcaa ccggatcagg
     1561 cagcccagat gcgtctattc taggatccat cattcctcgc acgccgccga cctgcatggt
     1621 gttgcctccc cccgacacgg caaggacaac cgcatcctca gcctgtttcc ttgaccggac
     1681 gtgtcgcagc acgtaaagtg tacgtacact gtgtccgggg atgaggagag accgtggcag
     1741 cgcgagcgaa ggcgaaatca acgcaacagg gaacgacccg gcgggtgagt gtcacggcgc
     1801 caccgaaacc ggcgcggccg tcgcggattg gcaggctggc ccgctgggtt ggtggctggt
     1861 tcgcgagcat cgacattccg cagcggttgc gcgaggagtt gatcgccttt gccgtcttta
     1921 cgctggcggt tgtcgcggcg ttgccgctgg tcggattggc gaaggggagc gtcctcgact
     1981 acctcggcag cgccacgcgg gcgttgttcg ggatggcggc gatcgtggtg ccgctgggcc
     2041 tgtcggtcat tgccgtcgag gtctggagag cgggagaggc gggccagcgc gttcgccgga
     2101 ttgtcggcgg cgtcgtaacg gtcgcctcga tcgtgggtct gatgacgctc ggtcagaccg
     2161 acgtcgatgt cgcgcagaca ggtggcgccg ccggctgggc ggtcgcgacc ggcctgatgc
     2221 ggctgctcgg tgaggtcggc gcgggcctga cgctgatcgc actcggcttc gcgggagtgt
     2281 tcctcgtcac cggctacacc gttcgggaaa tcagcgaatc ctggcagcgg gtgcgcccgc
     2341 gtcgtcgcta tgctgctgcc ggttgggagc ttgacgacgc ccagggtgaa tcagcgatcg
     2401 agtccgcgac cgagccgttc cagcgcgctg ccacccagga aatgtcggcg gctactgtcg
     2461 cgccggtcgc cgaggcggtc gcggcagcgc cggtcatcaa ggttccgaag ccggatcggc
     2521 ccgcaccggt ggcgaagccg aaggacgaca cgcctgaggc cgaaccggag cagcgggcgg
     2581 ccccgcgtgg caagtggaag ctgccgtcga tcaatcagct tgccctgtat gagaactctg
     2641 agccggattc cagcgatttg gaagccaagg cgaagcgaat tgaagagacg ctttcgtcgt
     2701 tcaaggtcga ggcgaaggtc cgcgaggtct ttcccggccc ggccgtgacg ctcttcaccg
     2761 tccagcccgg ttcgggcgtc aaggttcgcc gcatcacgga gttgcagaac gacctggctc
     2821 tggcgctggc agcgccgtcg atccggattg aagcgccggt ccctggcatg gcgcgggtcg
     2881 gaatcgagat tcccaacgcc agcatcgcga ccgtcggtct gcgtgagaca ctggagtcga
     2941 gtggcttcac caagtcgaac gccaagctgc ccctcccgct cgggcgcgac gtcaacggcc
     3001 gctacatcgt cggcgatctc gccaagatgc cgcacctgct gattgccggt tcgaccggct
     3061 caggcaagag cgtctgtctg aacggcatca tcgcgacctt cctgctgagc aagacgccgg
     3121 atgacctcaa gatgatcatg atcgacccga agatggtcga gatggccggt tacgagggcg
     3181 ttccgcacct caaggcgccg gtcgtgaccg agatgaacaa ggtcgtctcg gcgttgcggg
     3241 ctgtcctgca cgagatggaa cgtcgctacc agctcttctc gcggctaggt gttcgtaaca
     3301 tcgacggcta cgaactgcgc cgctcgaccg agccgacgct tgagaagatg ccgtacatgg
     3361 tcgtcatcat cgacgagctg gccgacctga tgatgacgac gccggacgag gtcgagacga
     3421 ttctggtccg gctggcccag atggcgcgcg cgacgggtat tcacctgatc atcgcgaccc
     3481 agcgtccgac cgtcgatgtc ctgacgggcc tgatcaaggc caacgtcccg gcccggatcg
     3541 cgttcgcggt gacgtcggtg acggatagcc gcgtgattct ggatgtcgcc ggcgcggaac
     3601 ggttgcttgg ccgtggcgac atgctgttca tgccggccga cgccgccaag ccgtaccgca
     3661 tccagggttc atacatcgac gatcaggact tgcagaagat cgtcaagcac tggcggctgc
     3721 tcgcgccgga gacgatttac gagccggagt gggcgcagct gccggctgac gacgaggcgg
     3781 atgaggacag cgaggatccg ctgatggagc aggcgctgaa catcgtccgc cagcagggaa
     3841 ccgcttcggc ctcaatgctg caacggcggc tgcggatcgg ctacaaccgc gctgcacgat
     3901 tgatcgagca gatggaggaa gagggcatca tcgggccggc ggatggctcg aaggtccggc
     3961 cggtcatctc gatggacgac tgggacgagt aaatccggtc ggtgaccacc gcaatgtata
     4021 ctttttgccg gatgcagcgg ctggtggagg ctcggattgc ggcaatcgag aattacagca
     4081 gagccagttg ccaggttgat cgacgagctc ggcc
//
LOCUS       DASUGJ010000120         6015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_433052, whole genome shotgun sequence.
ACCESSION   DASUGJ010000120 DASUGJ010000000
VERSION     DASUGJ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433052"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFV93_02695"
     CDS             <1..570
                     /locus_tag="VFV93_02695"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5164279.1"
                     /translation="VCAVDQDVNAFSATPSDGVGSTPVVPGLGLICSGRGSQTWLIPG
                     HASALEGGKRPRLTPNPAMAFRDGKLFMPFGTPGGDMQPQSMMQTFINIVEFGMDVQQ
                     AIEAPRFGTYSHPNSFWPHAYLPGRLSLEARISRTVGDDLAARGHDLEWWPEWTRVAG
                     NVCAIRVDHERETLEAGADARAEAYAAGW"
     gene            662..1933
                     /locus_tag="VFV93_02700"
     CDS             662..1933
                     /locus_tag="VFV93_02700"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX5164280.1"
                     /translation="MGLAETSSSLVPFRGVRFRAGLPGGLAATLGPPDDIDSSDEARA
                     FVQGKPFNAVRLEIEDDSEPLRFQSARALLDRWRQDDVVGADPTPGYYVYEQAFSDHG
                     ERRIRRGIIGLVPLDAPDVCVLPHEETWEENRQRRYQLLCDLEAGISPVFLIYDGPET
                     AQMLEAIVQRPPLAVAEDGIDGHRLWRVSEPDEVARIAASMREQHFIIADGHHRFAAA
                     QRYHQERGTSESALVLACCIEAHDPGIVIRPFHRIVSLPGGCDVNEWLTTLAAWFDVE
                     EAPVGNRSGQDLAASLPAGTLPAAGLILDGGLRFSTLRLRDWDAIESLIPAGVTGPAR
                     RLDVTILSELLIHQTLGISADAEPDRIDYTTDAAEAVAATQRDTGTIAILLRPIALQQ
                     VLAVARAHGTVPAKSTSFIPKVPIGLVLHEY"
     gene            2346..4934
                     /gene="mutS"
                     /locus_tag="VFV93_02705"
     CDS             2346..4934
                     /gene="mutS"
                     /locus_tag="VFV93_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5164281.1"
                     /translation="MTPVRRQYLSIKRQYPEIIVFFRMGDFYETFDGDAELVSSVLGL
                     TLTSREMGRGNRVPMAGIPYHAAEGYLARLLAAGHKVAIAEQTEKPNGKDLVDRRVTS
                     VVTPGTVTDPAFVRGAGNTYVVALVGESGSAGIAYADVTTGEFGTTRLSAENDAGLAE
                     AVRRELLRLGPAEIVTREDEPLLTCLGDDTARSTMPNNAWRFDDACETLLEHFSVGSL
                     EPFGCEGEPLAVRAAGALLAYLQSTQFNSLRQITTLVTYSTSRFMSLDAQTRRNLELV
                     ESSSRGGGPTLFTTLDETRTPLGARLLRQWIGQPLLERAEIERRHDGVGWFVAEPLIR
                     AKVREALRGIADIERVINRVVNSQAGPREMASLGASLARLPGLVSLLDTGDQPEIVGS
                     VPLAERAADEICRAIADDPPTLLANGGAIREGYSAELDGLRRTLARDREYIMNIEAIE
                     RERSGIKGLKVGYNKVFGYYIEVSNANREPIPDDYIRKQTLVNAERYITPDLKEAEQR
                     VIAAEDRITAAEADAYGRLLSTVAAEADTIRAAARAVATIDTLAGLAEIAATRGYVRP
                     ELIDDDSLRIDGGRHPIVERQLERGAFVPNDTTLSDDDGRIAILTGPNMAGKSTYLRQ
                     VALIVLLAQLGSFVPAERARLGVVDRIFTRIGAQDDLASGQSTFMVEMLETAAILNHA
                     SPRSLVVLDEIGRGTSTYDGLAIATAIVEYLHNTPRLNPKTLFATHYHELTALAEILP
                     RLRNYRLDVLEAGENVTFLHRVVPGGADRSYGVYVAQLAGMPRQVVRRAGEVLRELER
                     DSNTPGQREARVAAVTQQPAAPVQLTLFGAPHPAVERLKALEVDSLSPLDALTELYEL
                     KKLADG"
     gene            5072..>6015
                     /locus_tag="VFV93_02710"
     CDS             5072..>6015
                     /locus_tag="VFV93_02710"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HEX5164282.1"
                     /translation="MDAFETLKTLMELPGPTGRERPVMDWLRDAWEPQVERVWSSKVG
                     NLLAHVGGQGPKLLIQGHADELSFVVKFIDDNGFLWLSNGQAPNTDVTHRFPVGQPAL
                     VISRTARIDGLFAAASGHILTRQQRERYQVSTDDVFVDIGASSRDEAVEMGVHVGAGV
                     IWNPPVRRLGTRIYGKAIDDRVALALMTLLLDGLDVSTLTYDLYFAATIQEEIGLGGA
                     LSLRHDVDADLAIALDNGLVGDIPTVATRNMPVVLGGGPTLVYKDAGIHYDVRILDHL
                     SDIAERAGIPVQHAVYEQIGSDGSALIKQGIPTALLGPS"
BASE COUNT         1015 a         2064 c         1992 g          944 t
ORIGIN      
        1 gtctgcgccg tcgatcagga cgtcaacgcc ttctcggcga cgccaagcga tggcgtcggc
       61 agcacgccgg tcgtgccggg gctggggctg atctgctccg gacgcggctc gcagacgtgg
      121 ttgatccccg gccacgcctc ggcgctggag ggcggcaagc gcccgcgact gacaccgaac
      181 ccggcgatgg ccttccgcga cggcaagctg ttcatgccgt tcggtacacc cggcggcgac
      241 atgcagccac agtcgatgat gcagaccttc atcaacatcg tcgagttcgg gatggatgtc
      301 cagcaggcga ttgaggcgcc gcgtttcggc acctacagcc accccaactc gttctggccg
      361 cacgcctacc tgcccggccg gctgagcctc gaagcgcgca tctcccgcac ggtcggcgac
      421 gatctggcgg cgcgcgggca tgacctggag tggtggccgg agtggacccg cgtcgccggc
      481 aacgtctgtg ccatccgcgt cgaccacgag cgggagacgt tggaagccgg cgccgatgcg
      541 cgtgcggaag cgtacgcggc cggttggtag cggtccggga atatccccag cccgtcgcat
      601 gctacgatca agggctgatc gggctgcgac gggtgcgtgg gaggcgctgg cgagagacgg
      661 tatggggtta gcagagacat catcctcgct ggtgccattt cgcggcgtcc gtttccgtgc
      721 cggtctgccg ggcggcctgg cggcgacgct ggggccacct gacgacatcg actcttccga
      781 cgaggcgcgc gccttcgtgc agggcaagcc gttcaacgcc gtccggctcg agatcgagga
      841 cgacagcgag ccgctgcgct ttcagtcggc ccgcgcgctg ctcgatcgct ggcggcagga
      901 cgacgttgtg ggcgccgatc cgacgcccgg ttactacgtc tacgagcagg cgttcagcga
      961 ccacggcgaa cggcgaatac ggcgcggcat catcgggctg gtcccgcttg acgcaccgga
     1021 tgtctgcgtc ctcccccatg aggagacctg ggaagagaac cgccagcggc gctaccaact
     1081 cctctgcgat cttgaggcag gcatcagtcc ggtctttctg atctatgacg gccctgagac
     1141 ggcgcaaatg cttgaggcga ttgtccagcg accgccgctg gccgtcgccg aagacggcat
     1201 cgacggacac cgactctggc gcgtctccga gcccgacgag gtcgcgcgga tcgcggcgtc
     1261 aatgcgggaa cagcatttca tcatcgccga cggccaccac cgcttcgccg ccgcccagcg
     1321 ttaccaccag gaacggggca cgtccgaatc cgccctcgtc ctggcctgct gcatcgaggc
     1381 gcacgacccc ggcatcgtca ttcgcccctt ccaccgcatc gtctccctac cggggggctg
     1441 tgatgtgaac gagtggctga cgacgctcgc ggcgtggttc gatgtcgaag aagcgcccgt
     1501 cggcaatcgc agcggtcaag acctcgccgc ctcgctcccg gccgggacac ttcctgccgc
     1561 cggattgatc ctcgatggcg gcctccgctt ctcgacacta cgccttcgcg attgggacgc
     1621 tatcgagtcg ctgatcccag ccggcgtgac cggacccgcc cgccggctcg acgtcacgat
     1681 cctctcggaa ctgctgatcc accaaacgct gggcatctcc gccgacgccg aacccgaccg
     1741 aatcgactac acgaccgacg ccgccgaggc ggttgcggcg acgcaacgcg acaccggcac
     1801 gatcgcgatc ctgttacgac cgatcgcgct acaacaggtc ctcgcggtag cgcgagccca
     1861 cggcacggtc ccggcaaaat cgacgtcgtt catccccaag gtcccaattg gtctggttct
     1921 gcacgagtat taggatgcgg tcgacatcct ttgcgctgcc aatacgcgaa gcgaaccaca
     1981 cgctggaccg tgccgaggac cgcacacgcc acccctgtca tcctaagccg cagcgaagga
     2041 tcgcctggcg ttggagcgac ggacggctgc ccaggatacg aacggtcggc cgccgaacca
     2101 ccctctcgcg accctcacgc ctggcgatcc ttcgctgcgg ctcaggatga caggggtggc
     2161 gtgttgggtg cctcatcggc gccgtgcgtc tggctcgtgg cactcgccgc gcattgaggt
     2221 cggcacacca cgacatcgcg tgttaggcta cagcctcact gtacgtacgt tgccactgtc
     2281 gaggaaggac agatgagcca gcacgcgcga ctcaggacgc agcacgcatc acgcagcacc
     2341 taccgatgac gcccgttcgc cgccagtacc tgtcgatcaa gcgccagtat ccggagatca
     2401 tcgtcttctt ccggatgggc gacttctacg agacgttcga cggtgacgcc gaactggtgt
     2461 cgagcgtgct cgggctgacg ctgacttcgc gcgagatggg gcgcggcaac cgggtgccaa
     2521 tggcaggtat cccctaccac gcagccgaag ggtatctggc acggctgctg gcggccggtc
     2581 acaaggttgc gatcgccgag cagaccgaga agccgaacgg caaggatctg gttgaccggc
     2641 gggtgacgag cgtcgtcacg cccggcaccg tcacggatcc ggccttcgtc cgcggcgccg
     2701 gcaacaccta cgtcgtcgcg ctggttggtg agtcaggttc ggccgggatc gcctacgccg
     2761 acgtgacgac cggtgagttc gggacgacgc ggctctcggc cgagaacgac gccgggctgg
     2821 ccgaggcggt tcggcgcgag ttgctacggc tcggtccggc cgagatcgtc acgcgggagg
     2881 acgagccatt gctcacctgc ctcggcgatg acacggcccg ctcgacgatg ccgaacaacg
     2941 cctggcggtt cgacgacgcc tgcgagacgt tgctggagca cttcagcgtc ggctcgctgg
     3001 agccgttcgg ctgcgagggc gagccgctgg cggtccgggc ggccggcgcg ctgctggcgt
     3061 acttgcaatc gacccagttc aactcgctcc ggcagatcac gaccctcgtc acctactcga
     3121 cctcgcgctt catgagcctc gatgcccaga cgcggcgcaa tctggaactg gtcgagtcgt
     3181 ccagccgggg cggtgggccg acactcttca cgacgctcga tgagacacga acgccgcttg
     3241 gggcgcgctt gctgcgccag tggatcggcc agccgttgct ggagcgcgcc gagatcgagc
     3301 gacggcacga cggcgtcggc tggttcgtcg ccgagccgct gatccgagcc aaggttcgcg
     3361 aggcgctgcg cgggatcgcc gacatcgagc gggtcatcaa ccgcgtcgtc aacagccagg
     3421 ccgggccgcg cgagatggcc agcctcggcg cgtcgctggc gcggctgccg gggttggtgt
     3481 cgttgctcga tacgggcgac cagccggaga tcgtcggcag cgttccatta gcagaacggg
     3541 cggcggatga gatctgccgc gccatcgccg acgacccgcc gaccctcctc gccaacggcg
     3601 gcgcgatccg cgagggctac tcggccgagc tggacgggct gcggaggacg ctggcgcgtg
     3661 accgcgagta catcatgaac atcgaggcga tcgagcggga gcgcagcggc atcaaggggc
     3721 tgaaggtcgg ctacaacaag gtcttcggct actacatcga ggtctccaac gcgaaccgcg
     3781 agccgatccc cgacgactac atccgcaagc agacactggt caacgccgag cgctacatca
     3841 cgcccgacct caaggaggcc gaacagcgcg tcatcgctgc cgaggaccgg atcaccgctg
     3901 ccgaggccga cgcatacggc cgcctgctga gcacggtcgc cgctgaagcg gacacgatcc
     3961 gggcggcagc gagggcggtc gcgacgatcg acacgctggc cggactggcg gagatcgcgg
     4021 cgacgcgcgg ctatgtccgc ccggagctga tcgacgatga cagcctgcga atcgacggcg
     4081 gccgtcaccc gatcgtcgag cgccaactgg agcgtggcgc gttcgtcccg aacgacacga
     4141 ccctctccga cgacgacggc cggatcgcga tcctgaccgg cccgaacatg gccgggaagt
     4201 ccacgtatct gcggcaggtc gcgctgatcg tgctgctggc gcagctcggc tcgttcgtgc
     4261 cggccgaacg agcgcggctg ggagtagtag atcgcatctt cacgcggatc ggtgcccagg
     4321 acgacctcgc cagcggtcag tcgaccttca tggttgagat gctggagacg gctgcgatcc
     4381 tcaaccacgc atcgccacgc tcgctggtcg tcctcgacga gatcggccgc ggcaccagca
     4441 cctacgacgg gctcgcgatc gcgacagcga tcgtcgagta cctgcacaac acgccgcgcc
     4501 tcaaccccaa gacgctcttc gcgacccact accatgagtt gacggcgctg gccgagatcc
     4561 tgccgcgcct gcgcaactac cggctcgacg tccttgaagc cggcgagaac gtcaccttcc
     4621 tccatcgggt ggtcccgggc ggggccgacc gctcctacgg cgtctacgtc gcccaactgg
     4681 ccggcatgcc ccgccaggtc gtccgccgcg ccggcgaggt gctgcgcgag ctggagcgcg
     4741 acagcaacac ccccggccag cgcgaggccc gcgtcgccgc cgtcacccag cagccggccg
     4801 ccccggtcca gctaaccctg tttggcgcgc cccatccggc cgtcgaacgc ctcaaggcgc
     4861 tggaagtgga cagcctctcg ccactggatg ccctgaccga gttgtatgag ttgaagaagc
     4921 tggcggacgg gtagggaccg atcctgtatc ggcccctacc cggggagaac cccctgcatt
     4981 gcgaattaca gcagttaggc tgcgcggggt gatccgtgaa ttattcgcgc taccattgca
     5041 accgaccgca aatggtggag aaggggagca gatggacgca ttcgagacgc tgaagacgtt
     5101 gatggaactg cccggaccga ccgggcgcga gcggccggtc atggactggc tgcgcgacgc
     5161 ctgggaaccg caggtcgagc gggtctggtc cagcaaggtt ggcaatctgc tggcgcatgt
     5221 cggcgggcag gggccgaaac tgctgattca ggggcacgcc gacgaactct cgttcgtcgt
     5281 caagttcatc gacgacaacg gcttcctctg gctctcgaac ggccaggcgc cgaacaccga
     5341 cgtcacccat cgctttccgg tcggccagcc tgctctcgtc atcagccgga cggccagaat
     5401 cgacggtctt ttcgcggctg ccagcggcca tatcctgacc cgccagcagc gtgagcgcta
     5461 ccaggtcagc accgacgatg tcttcgtcga tatcggcgca tcctcacgcg atgaggctgt
     5521 cgagatgggc gtccatgtcg gcgcgggcgt gatctggaat ccgccggtcc gcaggctcgg
     5581 gacccgcatc tacggcaagg cgatcgacga tcgggtcgcg ctggcgctga tgacgctgct
     5641 gctcgacggg ctggatgtct cgacactgac ttacgatctc tacttcgcgg cgacgatcca
     5701 ggaggagatc ggtctgggcg gggcgctctc gctgcgccac gacgtcgatg ccgatctggc
     5761 aatcgcgctc gacaatggcc tggttggcga tatcccgacc gtcgcgaccc gcaacatgcc
     5821 ggtcgtcctc ggcggcgggc cgacgctggt ctacaaggac gccggcatcc attacgacgt
     5881 gcgtatcctc gaccacctgt ctgacatcgc cgagcgggca ggcatcccgg tccagcacgc
     5941 cgtctacgag cagatcggca gcgacggctc ggccctcatc aagcagggca tcccgacggc
     6001 gctgctcggc ccctc
//
LOCUS       DASUGJ010000121         4832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1266158, whole genome shotgun sequence.
ACCESSION   DASUGJ010000121 DASUGJ010000000
VERSION     DASUGJ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266158"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFV93_02715"
     CDS             <1..441
                     /locus_tag="VFV93_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEX5164283.1"
                     /translation="GQTGLSNLQRRWEGQMVDTTQELEVIQTAKAAAPIGPYSQAIRA
                     NGFIFVAGEKGIDPTTNAIVAGGIPAETRQTLENIRNILEEAGSSMSRVVATTVYLTD
                     INTFGEMNKVYAEYFTSNPPGRTTVGVTSLPAGAQVEITVTALA"
     gene            464..1120
                     /locus_tag="VFV93_02720"
     CDS             464..1120
                     /locus_tag="VFV93_02720"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX5164284.1"
                     /translation="MTVTGSGNVTSETRPLADWTAVALACPGTLDLQIGPNQGITIEA
                     EDNILPLIETRVDGGGLQIRFQPAAQSIRPTQPIRFRAATPAIDGLAVSGSGSIHAPA
                     IEGATLTLGTSGSGSIDVASASTGALDARISGSGSVTVRGDTRDCDVRVSGSGDLNAR
                     DLACHRAHVNVSGSGSVMLRVEREIEGRISGSGSIVYSGQPVTSIRTSGSGRAVQMPG
                     "
     gene            1209..2186
                     /locus_tag="VFV93_02725"
     CDS             1209..2186
                     /locus_tag="VFV93_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5164285.1"
                     /translation="MGWWRAGERRSWGVVLVGIVLASLLAGCGNDGSGGYQGEIEIDG
                     SSTVFPISQAVAEEFFLENRGTKFTVGASGTGGGFEKFCRGDIDISDASRPIKPDEAA
                     LCAGNGIEYSELTVAADGLSVIVHPDNDWADCLTVEQLRQLWMPGSTVKRWSDLDPSW
                     PDKQIDLYGPGTDSGTFDYFTETINGEAGASRADYSASEDDNTLIQGVEGDRYALAYL
                     GYAYYSENADRLKVLAIDGGNGCVAPSPDTVRDGSYAPLSRPVFIYVNNAGLTANDSP
                     DLETPGPSSTLAAFVIYYLTEGIGVIPFVGYVPYDEAFYEEQLRAIGLR"
     gene            complement(2312..2737)
                     /locus_tag="VFV93_02730"
     CDS             complement(2312..2737)
                     /locus_tag="VFV93_02730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX5164286.1"
                     /translation="MAVALDTSTQRGQHVETRLNDDVIAWLTTVRPSGQPDSVPVWFI
                     WQDGRIIVYSRPDQTKLRNLAENPKVTLTIDNTENGADVIRIEGTAEHDSSYPPLHTV
                     PAYVAKYADRIAYIGYGTPENFARTYSAAIVITPTRFRV"
     gene            3029..>4832
                     /locus_tag="VFV93_02735"
     CDS             3029..>4832
                     /locus_tag="VFV93_02735"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5164287.1"
                     /translation="MPSANLIGQIIGSYRIDALLGSGGMGHVYRATHVQLRRPVAIKV
                     LDSDAATDPTFQARFLREARAAASLSHPNIVHVYDFGEQDGRPFLAMELVPDGSIGEL
                     QRSGPLDLVVGLDLVRQAAQGLAAAHEREMVHRDIKPDNLLLAARTPAAPELAPYTVK
                     IADFGLARLADSGPLTQAGVAIGTPAYMSPEQCQGLQVDPRTDIYSLGIVLYEIATGS
                     RPFNVRSLSDAVYQHVHVAPPPPRQVAPDLSADVEQIINRCLAKQPADRYQRAGALVA
                     SLDTAIAREVDRRRTGVLPDIAAGAAPAAGLGMALSAGAGGQSASVLPAAAGAGGLAG
                     APGAGLPGAPVAGGIPGAPAGPGLGGMQSAPAAAGPGAGGMPGLHGTGSVASGPGAGG
                     MPGVHGSGSIASGPGAGGIPGGHGANLAAQGPGAGGFGGSPPPAVGRVNLPANDPGGI
                     TSPAMTGSGTAGNAGLPPASIHSVIGSVPALPPLDQPAHAGRASIGSVIAKVPLAILA
                     VAAVALIAVGAYLTGINPIGGGDDDGNDPPLAVVADASATSTATQAALAAVGTGTSTP
                     TTVEINGPAATATATTAPANATAPPRQIVPTGATA"
BASE COUNT          805 a         1623 c         1645 g          759 t
ORIGIN      
        1 gggcagaccg gactcagcaa cttgcagcga cgatgggaag ggcagatggt cgacacgaca
       61 caggagctcg aagtcatcca gaccgcgaag gcggcagcac cgatcgggcc atactcgcag
      121 gcgatccgcg ccaacggctt catcttcgtc gccggtgaga aggggatcga cccgacgacg
      181 aacgcgatcg tcgcgggcgg tattccagcc gagacacgcc agacgctgga gaacatccgc
      241 aacatcctcg aagaggccgg ttcgtcgatg tcgcgcgtcg tcgcgacgac ggtctacctg
      301 accgacatca acacctttgg cgagatgaac aaggtctacg ctgaatactt caccagcaac
      361 ccgccggggc ggacgaccgt cggtgtcacg tcgctgccgg ccggcgcgca ggtcgagatc
      421 actgtcacgg cgctagcctg acgaacggcg aacgggaggg cgaatgacgg ttaccggctc
      481 cggcaatgtc acgtctgaga cacgaccgct ggcggactgg accgccgtcg ccctcgcctg
      541 ccccggcacg ctcgacctgc agatcgggcc gaaccagggc atcacgatcg aggcggagga
      601 caacatcctg ccgctgatcg agacgcgggt tgatggtggc gggctgcaga ttcgtttcca
      661 gccggccgcg cagtcaatcc ggccgacgca gccgatccgc ttcagggccg cgacgccggc
      721 catcgacggg ctggccgtct ccggctcggg cagcatccac gcaccggcga tcgagggagc
      781 cacgcttacg ttgggaacca gcggctcggg aagcatcgac gtcgcatcgg caagcaccgg
      841 ggccctcgac gcgcgcatct ctggatcggg gagcgtgacg gttcgcggcg acacgcgcga
      901 ctgcgacgtg cgcgtctctg gctctggcga cctgaacgca cgcgacctcg cctgccatcg
      961 cgcccatgtc aacgtctccg gctcgggttc cgtgatgctg cgggtcgagc gggagatcga
     1021 gggccggatc agcggcagtg gctcgatcgt ctacagcggg cagccggtaa cctcgatccg
     1081 cacctccggc tccggccgcg ccgtccagat gcccggttga cccggcccaa catcgtcctg
     1141 accggctgga ttgcttacca ttcgcgcggg atgtccggaa atggcggcgt ttggtgaggc
     1201 ggggtgtcgt gggctggtgg cgtgctgggg agcgccggtc gtggggtgtc gtgcttgtcg
     1261 ggatcgtgct ggccagtctg ctggccggtt gcggcaacga cggcagtggc ggctaccagg
     1321 gcgagataga gatcgacggg tcgagcaccg tcttcccgat ctcacaggcg gtggcagaag
     1381 agttcttcct tgagaaccgc gggacgaagt tcacggttgg cgcatccggt accggcggcg
     1441 gcttcgagaa gttctgccgg ggcgacatcg acatcagcga cgcctcgcgg ccaatcaaac
     1501 cggacgaggc cgccctctgc gccggcaacg ggatcgagta cagcgagctg acggtcgccg
     1561 ccgatggcct ctcggtgatc gtccaccctg acaacgactg ggccgactgc ctgaccgtcg
     1621 agcagctgcg gcaactctgg atgcccggca gcaccgtcaa gcgctggagc gacctcgatc
     1681 cctcgtggcc cgacaagcag atcgacctct acggccccgg caccgactcc ggcacgttcg
     1741 actacttcac ggaaacgatc aacggcgagg ccggcgccag ccgcgccgac tacagcgcca
     1801 gcgaggacga caacacgctg atccagggcg tcgaaggcga ccgctacgcg ctggcctacc
     1861 tcggctacgc ctactacagc gagaacgccg accggctcaa ggtgctggcg atcgatggcg
     1921 gcaacggctg cgtcgcgccg tcgcccgaca ccgtccgcga cggcagctac gcacccctct
     1981 cgcgcccggt cttcatctac gtcaacaacg ccggcctgac cgcgaacgac tcacccgatc
     2041 tggagacacc cggaccgtcc tcgacgctgg ccgccttcgt catctactac ctgaccgaag
     2101 gcatcggcgt catccccttt gtgggctacg tcccgtatga cgaggcgttc tacgaggaac
     2161 aactgcgggc gatagggctg aggtgatggg tgtgacggac gagttggaat cgctgggcgg
     2221 gatacggccg tgatttcacg gcacgctggc cggcgtccac tctatccacc atgacccatc
     2281 aaacggtcat gtggtgaagg cgaagctggt gctatacacg gaaccgcgtc ggggtaatca
     2341 caattgcggc cgagtaggtc cgggcgaagt tctcgggggt gccgtagccg atgtaggcga
     2401 ttcggtcggc atatttcgcg acgtaggcgg gaacggtgtg caggggtggg tagctgctgt
     2461 cgtgctcggc ggtgccctcg atccggatca cgtcggcgcc gttctcggtg ttgtcgatcg
     2521 tcagcgtgac cttcggattc tcggccaggt tgcgtagctt ggtttgatcg ggccggctgt
     2581 agacgatgat ccggccatcc tgccagatga accagaccgg aacgctgtcc ggctgaccgc
     2641 tcggcctgac cgtcgtcagc caggcgatga catcgtcgtt cagtcgtgtc tcgacgtgct
     2701 gtccgcgctg cgtcgaggta tccagcgcta ccgccatctg gtgaggttcc cttcgttcgt
     2761 cgttatcaat cgatctcgcg ccaaccggcc actcgttgca ggtagcgatg gtcaggtgtg
     2821 gtttattccg catcagctgg acgctcgggc cgtctgttaa ccagattact actgatctgt
     2881 cacctggttg gctttcgcat tttgcgacat ggcgctacaa tcgaagcact tcgacgcaag
     2941 gcgatacggg cgcggactgg cctcgctttc cgatggttgc ggcaccacgg cagccgtttc
     3001 tcctgaattg caaccagagg acgattgcat gccctcagcg aacctcatcg gacagatcat
     3061 cggcagctac cggatcgatg cgctgcttgg ctccggcggg atggggcacg tctaccgtgc
     3121 aacccacgtc cagctacgcc ggccggtcgc gatcaaggtg ctcgacagcg acgccgcgac
     3181 tgacccgacc tttcaggcgc gcttcctgcg cgaggctcgg gcggcagcgt cactgagcca
     3241 tcccaacatc gtccatgtct acgacttcgg cgagcaggat ggccggccgt ttctggcgat
     3301 ggagctcgtc ccggatggct cgatcggcga attgcagcgc tccgggccgc tcgatctggt
     3361 cgtggggctc gacctcgttc gccaggcagc gcaggggctg gccgccgccc acgaacgcga
     3421 gatggtccat cgcgatatca agccggacaa cctgctgctg gcagcgcgaa cgccggcagc
     3481 gccggaactg gcgccataca cggtcaagat cgccgacttc gggctggcac gactggccga
     3541 tagcggcccg ctgacccagg caggcgtcgc aatcggcacg ccggcctaca tgtctcccga
     3601 gcagtgccag gggctgcagg tcgacccgcg caccgacatc tactcgctgg ggatcgtcct
     3661 ctacgagatc gcaaccggca gccggccgtt caacgtccgc agtctgtccg atgcggtcta
     3721 ccagcacgtc cacgtcgcgc cgcccccgcc gcgtcaggtc gcgcccgacc tctcggccga
     3781 tgtcgaacag atcatcaacc gctgtctcgc caagcagccg gctgaccgct atcaacgggc
     3841 cggcgcgctg gtcgcctccc tcgacaccgc gatcgcgcgg gaggtcgacc ggcgacggac
     3901 tggagtcctg ccggatatcg cggccggagc ggcaccggcg gccggcctgg ggatggcgct
     3961 gagcgcagga gcaggcgggc aatccgccag tgtcctgcca gccgcggcag gagccggcgg
     4021 gctggccggc gcgccgggcg cgggcttgcc gggagctccc gtcgcgggcg ggataccggg
     4081 cgcaccggct ggaccgggcc tcggtgggat gcagagcgcg ccggcggcgg ctgggcctgg
     4141 agccgggggt atgcccggcc tgcatggcac cggatcagtt gcaagcggac cgggggccgg
     4201 cggaatgcca ggtgttcacg ggtccggatc gatcgcaagc gggccgggcg ccgggggcat
     4261 acccggtggg catggcgcga acctcgccgc ccagggaccg ggcgccggcg gcttcggcgg
     4321 atcaccgccg ccggctgtcg ggcgcgtcaa cctgccggcc aacgatccgg gcgggataac
     4381 gtctccggcg atgactggat caggcacagc cggcaacgcc ggcctgccac cggcctcgat
     4441 ccactcggtg atcggctcgg tcccggcgct gccaccgctt gaccagcccg ctcatgcagg
     4501 gcgggcctcg atcgggagtg tcatcgcgaa ggtgccgctc gccatcctgg cggttgcggc
     4561 cgtggcgctg atcgcggtag gagcctacct gaccggtatc aacccaatcg gcggtgggga
     4621 cgacgacgga aacgacccgc cgctggccgt cgttgcggac gccagcgcga catcgacggc
     4681 aacccaggct gcgctggcag cagtcgggac cggcaccagc acgccaacca ccgtcgagat
     4741 caacggcccg gcggcgacgg ccacggcgac gaccgccccg gccaacgcga cggcgccacc
     4801 gcgccagatc gtgcccaccg gtgcgacggc ga
//
LOCUS       DASUGJ010000122         5205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_155322, whole genome shotgun sequence.
ACCESSION   DASUGJ010000122 DASUGJ010000000
VERSION     DASUGJ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155322"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2391)
                     /gene="priA"
                     /locus_tag="VFV93_02740"
     CDS             complement(<1..2391)
                     /gene="priA"
                     /locus_tag="VFV93_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5164288.1"
                     /translation="MTYRVPAALDEIEVGQLIWVPLRRKLVLAVVVERHQRAPESGFE
                     IKDIHAPVEPSFCLTPIQWQLAVWMAEETICTLFEAASVMLPPGVSSRAVEYLSLRAT
                     PSAAALEALTPAQRRLVEFLEREGEVTLERAQRAQHSSLVSLIPILEEKGLLQKVARV
                     KHRDTQREEVRFQVRLLDGAAAPPERAPKQAEAFAWLSPRLRARSDRSLPIDSVLNIS
                     GIDRPTLRALADRGILAIEPLQAPSGPEKENGNTVRLTEEQRAAWDAIRTIRREEATR
                     TILLHGVTGSGKTELYLRLAADVLADRRSAVVLVPEIALSGQIAARFRERFGDRALVL
                     HSALGDRERYRNWQRAATGEPLVIVGPRSALFAPLADIGAIVLDEEHDGSYKQDSVPR
                     YHARTVAGKLAELHGSLLVLGSATPDVETYRASRRPDWSLLELRERVGQRIADDDGSV
                     RAHPIALPEARIVDMRAELRSGRDSIFSRALTDIISDRLELNQQVILFLNRRGASTFI
                     QCRSCGSVSSCPYCDIPLVYHRTIERLVCHRCGHRTTPPTICATCRSTSIGYYGTGTQ
                     RVETEIQSQFPGARVLRWDQDALRGKVSHESLLAKVMRHEVDIVVGTQMIAKGLDLPD
                     VTAVGVVNADTYLFLPDIRSAERTFQMLTQVAGRAGRRAAGGEVIFQTYSPEHYAVTS
                     AARHDYAAFYTEEIAFRRQHGYPPYKRLARLLIRNSDEAKARQTAEEMAARLEDELAA
                     RPQILGVDLIGPAPAFSTRVRGQYGWQLLIRGDQLISLLANVQFTPAWVVDVDPVNL"
     gene            complement(2609..3610)
                     /locus_tag="VFV93_02745"
     CDS             complement(2609..3610)
                     /locus_tag="VFV93_02745"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEX5164289.1"
                     /translation="MGFTDQPRTRFATLPTPLQFAPRLSEELGGPKIYLKRDDLTGLA
                     LGGNKTRKLEYLVADAQAQGATHLITVGAAQSNHARQTAAAARLAGMECHLVLNASST
                     DPEVQGNLLLDKLLGATVHVVVGGSEERQAKVDEVAADLSDSGAVPYVIPGGGSNGVG
                     SLGYVGAMLELSHQLWEQSVQPKAMYFAAGGGGTHSGIIVGAKLFNLDFAIIGVLVEG
                     KNEAGVERAYRVTGWTAERLGIDNPVAKSDIVCDDGHVGEGYGIPTEEGLDAIKLLAR
                     TEGVLLDPVYTSKAFAGMVADIRAGRYGADDAVVFLHTGGSPALFAQTDILLPVLDE"
     gene            3780..5141
                     /locus_tag="VFV93_02750"
     CDS             3780..5141
                     /locus_tag="VFV93_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5164290.1"
                     /translation="MAQATKTIEQQFHEEFTGSYQRSRHAMEIYPAGVTHDARFVKPF
                     PIYIERAQGSHKWDVDGHDLIDYAVGHGSLILGHNHPKVVEALKEQLDRGTHFGASHD
                     QELEWGEWVQRLVPSVQRLKFTASGTEATMLAIRAARAFSGKDKLLKFEGHFHGWHDT
                     AAPGEKVPFDAKSQPGIPQVTHDLTIVAPNDDIDFVEKALAKGDVAAVILEPSGASWA
                     TIPPREGFLKELREVTKKYDTILIFDEVITGFRWAPGGAQERFDVIPDMTTMAKIVAG
                     GLPGGCFGGRADIMSVFEFRDDPNHKKIVHPGTYNGNPLSAVAGYTCLKMVADPKIQQ
                     HADAMAARLRTGFNTVLVDRGVPGACWGESSVFHVGIGLDVPNLAAGDMRRPEGIDAT
                     TLKTGMGGKIKSSFVAGMMIEGSDITFGGGWLSVKHTPEDIDKTIEGFDRTVARMREE
                     GTV"
BASE COUNT          876 a         1672 c         1806 g          851 t
ORIGIN      
        1 caggttgaca ggatccacat ccacgaccca ggctggggtg aactggacat tggccagcag
       61 gctgatgagc tggtcgccac ggatcagcag ttgccagccg tactgaccgc gcacccgcgt
      121 cgagaaggcc ggggcggggc cgatcaggtc gacgccgaga atctgcgggc gggcggccag
      181 ctcgtcttcg aggcgggcgg ccatctcctc ggcggtctgt cgggccttgg cctcgtcgct
      241 gttgcggatc agcagccggg cgagccgctt gtagggcggg taaccgtgct ggcggcggaa
      301 cgcgatctcc tcggtgtaga aggcagcgta gtcgtggcgt gcggcgctgg tgacagcgta
      361 gtgctccggc gagtaggtct ggaagatgac ctcgccacca gccgcgcggc gaccggcccg
      421 gccggcgacc tgggtcagca tctggaaggt acgctccgcc gagcgaatgt cgggcaggaa
      481 caggtacgta tcggcattga cgacgccgac ggccgtgaca tccggcaggt cgagcccctt
      541 cgcgatcatc tgggtgccga cgacgatgtc gacctcgtga cgcatcacct tcgccagcag
      601 cgactcgtgg ctgaccttgc cgcgcaaggc gtcctgatcc caacgcagta cccgcgcgcc
      661 cgggaactgg ctctggatct ccgtctcgac ccgctgggtg ccggttccgt agtagccgat
      721 gctggtcgag cgacaggtcg cgcagatcgt cggcggcgtc gtccggtggc cgcagcggtg
      781 gcagacgagc cgctcgatcg tgcggtggta gacgagcggg atgtcgcagt acgggcaact
      841 ggagacgctt ccacagctcc ggcactggat gaaggtcgag gcgccgcgcc ggttgaggaa
      901 gaggatgacc tgctggttga gctccagccg gtcggagatg atgtcggtca gggcacggct
      961 gaagatcgaa tcgcggccgg aacggagttc ggcacgcatg tcgacgatcc gcgcttcggg
     1021 cagcgcaatc gggtgggcgc ggacgctgcc gtcgtcgtcc gcgatccgct ggccgacgcg
     1081 ctcacgcagt tccagcagcg accagtccgg ccggcgggac gcgcggtagg tctcgacatc
     1141 cggcgtcgca ctgccgagga cgagtagcga gccatgtagc tctgccagct tcccggcgac
     1201 cgtccgggcg tggtagcgcg gcacgctgtc ctgcttgtac gagccgtcgt gctcctcatc
     1261 aaggacgatc gcgccgatgt ctgccagtgg cgcgaacagg gctgagcggg ggccgacgat
     1321 cacgagcggc tcgccggtcg ctgcccgctg ccagttacgg tagcgctccc ggtcgccgag
     1381 ggcggagtgg agcaccagcg cgcgatcgcc gaaccgttcg cggaagcggg ccgcgatctg
     1441 gccggagagg gcgatctccg gcacgaggac gacggcagag cgcctatcgg ccagcacgtc
     1501 ggccgccagt cgcagataca gttcggtctt cccgctgccg gtgacgccat gcaggaggat
     1561 cgtccgggtt gcctcctcac gccggatcgt ccggatcgcg tcccaggcgg cgcgttgttc
     1621 ctcggtcagc ctgacggtgt tgccgttctc cttctcaggt ccggacggtg cctggagcgg
     1681 ctcgatcgcc agaatgcccc ggtcagccag tgcacgcagg gtcgggcggt cgatgcccga
     1741 gatgttgagg acggagtcga tcggcaagga ccggtcgctc cgggcacgca ggcgcggcga
     1801 gagccaggca aacgcttcgg cctgcttcgg cgctcgctcc ggcggtgctg ccgctccgtc
     1861 cagcaaccgg acctggaagc gtacctcttc acgctgcgtg tcacggtgct tgactcgtgc
     1921 gaccttctgg agcaaccctt tctcttccag gatcgggatc agcgagacca acgatgagtg
     1981 ttgcgcccgc tgggcacgtt cgagcgtcac ctcgccctcg cgctccagga actcgaccag
     2041 ccgccgctgg gcgggcgtca acgcctccag cgcggcagct gatggcgttg cgcgcagcga
     2101 gaggtattcg accgcccgcg agctgactcc cggcggtagc atcactgaag cggcctcgaa
     2161 gagcgtgcag atcgtctctt cggccatcca gactgccaac tgccactgga tcggtgtcag
     2221 gcagaacgac ggctcgacgg gcgcgtggat gtccttaatc tcgaagccgc tctccggcgc
     2281 tcgttggtgc cgctcgacga cgaccgccag caccagcttg cggcgcagcg gcacccagat
     2341 cagctgcccg acctcgatct cgtccagcgc agccggtaca cggtacgtca aggtcgcccg
     2401 ccgatcgctc agccgagcgt ccggcgcgac ctcaacgtac ggacccggtg tacggacaga
     2461 acatgcgttc atgtttagag tgtacaccgg aggtggccat aggcattagg ccttgggtct
     2521 caggagatga gccgtctgga tcgatcttca gcgtcgatgt agtgcgtacc aactcctaat
     2581 acctatggcc tggtgcctat ggcctcactc actcgtcaag caccggcagc agaatgtcag
     2641 tctgggcgaa cagggccgga gagccgccgg tgtggaggaa gacgacggcg tcgtcggcgc
     2701 cgtagcggcc ggcgcggatg tcggcgacca tgccggcgaa cgccttcgag gtgtagaccg
     2761 gatcgagcag gacaccttcg gtgcgcgcca gcagcttgat cgcgtcgaga ccctcctcgg
     2821 tcgggatgcc gtagccctcg ccgacgtggc cgtcgtcgca gacgatgtcc gacttggcga
     2881 ccgggttgtc gatgccgagc cgttctgccg tccagccggt gacgcggtag gcgcgctcaa
     2941 cgccggcctc gttcttgccc tcgacgagga cgccgatgat ggcgaagtcg aggttgaaga
     3001 gcttggctcc gacgatgatg ccgctgtggg tgccgccgcc accggccgcg aagtacatcg
     3061 ccttcggctg gaccgattgc tcccagagct ggtggctcaa ctccagcatc gcgccgacgt
     3121 aaccgagcga gccgacgccg ttcgagccgc cgccgggaat gacgtacggc acggcgccgg
     3181 aatcggacag gtcggcggcc acttcatcga cctttgcctg gcgctcctcg gagccgccaa
     3241 cgacgacatg aaccgtcgcg ccgagtagct tgtccagcag caggttgccc tgcacctccg
     3301 gatcggttga cgacgcgttc aggacaagat ggcactccat tccggccagc cgcgcggcgg
     3361 cggccgtctg gcgtgcgtgg ttggactgcg ccgcaccgac cgtgatcagg tgcgtcgcgc
     3421 cctgtgcctg cgcgtcggcg acgaggtatt cgagcttacg ggtcttgttg ccacccagcg
     3481 ccaggccggt caggtcatcg cgcttgaggt agatcttcgg cccgcccagc tcctcggaca
     3541 gacgtggcgc gaattgcagt ggcgtcggca acgtcgcgaa acgcgttcgc ggttgatcgg
     3601 tgaatcccat cggtgtccct cttcctcccc ggtctgaatc cgattcttgc ggcagatggt
     3661 acccgaagcc ggcgattggc ggtggacagg tggcgaaggg gtgggtctat aatggcgact
     3721 cgacagatga tcgcaatgag gtcgtcgcgg gagtgtatcg gggagggcag agcacagtca
     3781 tggcacaggc cacgaagacg attgagcagc agttccacga ggagttcaca gggtcgtacc
     3841 agcgctcgcg gcacgcgatg gagatctatc cggccggcgt tacccacgac gcgcgcttcg
     3901 tgaagccctt cccgatctat atcgagcggg cgcagggatc gcacaagtgg gatgtcgacg
     3961 ggcatgacct gatcgactac gcagtcggcc acggctcgct gattctcggc cacaaccacc
     4021 ccaaggtcgt cgaggcgctc aaggagcagc tcgaccgggg gacgcacttc ggcgccagcc
     4081 acgatcagga gctggagtgg ggcgagtggg tccagcggct ggtgccgtcg gtccagcggc
     4141 tgaagttcac ggcctccggg accgaggcga cgatgctggc gatccgggcc gcccgtgcat
     4201 tcagcggcaa ggacaagctg ctgaagttcg aaggccattt ccacggctgg catgacacgg
     4261 ccgcccccgg tgagaaagtg ccgttcgacg ccaagtcgca gccgggtatc ccgcaggtga
     4321 cgcacgatct gacgatcgtc gcgccgaacg atgatatcga cttcgtcgag aaggcactgg
     4381 ccaagggcga cgtcgcggcc gtcatcctcg aaccgagtgg cgcatcgtgg gcgacgatcc
     4441 cgccgcgcga ggggttcctg aaggagctgc gcgaggtcac gaagaagtac gacacgatcc
     4501 tgatcttcga cgaagtgatc accggcttcc gctgggcgcc gggcggcgcg caggagcgct
     4561 tcgacgtgat ccccgacatg acgacgatgg cgaagatcgt cgccggcggg ctgccgggcg
     4621 gctgcttcgg cggccgggcc gacatcatgt cggtcttcga gttccgcgac gacccgaacc
     4681 acaagaagat cgtccacccc ggcacctaca acggcaaccc gctctcggcc gttgccggct
     4741 atacctgcct gaagatggtc gccgatccga agatccagca gcacgccgac gcgatggcgg
     4801 cgcggctacg gaccggtttc aacacggtcc tcgtcgatcg cggcgtgcct ggcgcctgct
     4861 ggggtgagtc gtcggtcttc cacgtcggga tcgggctgga cgtgccgaac ctggcggccg
     4921 gcgacatgcg ccgtccggag gggatcgacg ctacgacact caagaccggc atgggcggca
     4981 agatcaagag ctcgttcgtg gccgggatga tgatcgaggg ctcggacatc accttcggcg
     5041 gcggctggct cagcgtcaag cacacgcccg aggacatcga caagacgatc gaaggcttcg
     5101 accggacggt cgctcgcatg cgcgaagagg gcacggttta ggcgggtggc aacccaatcg
     5161 caatccccaa tccggtaggg gcgatatcgc aatatcgccc ggagc
//
LOCUS       DASUGJ010000123         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1127540, whole genome shotgun sequence.
ACCESSION   DASUGJ010000123 DASUGJ010000000
VERSION     DASUGJ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127540"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFV93_02755"
     CDS             <1..968
                     /locus_tag="VFV93_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164291.1"
                     /translation="LAGALIVVIVDREATWGGLAAAPLVVGLLSVVAGTAVGYAGGTV
                     IPSRFAAPLMAVVFATAVLLVGTRSTAVAFLSPLAMDPRGSSPYDIFYQPPTIPLAQT
                     ALWLAGVAASACAASVLWRRRTALTWGTFLGALAVATTGAVLVTQAFVHPPWERIYAG
                     QPLADYELVCVERSIPVCLHPAYESRLEEYADQIDVLVEPLAGVPGGPVRAEQLPSRK
                     GPRLDGTLEIIPSGLIVAQATYDLVRAPGGVFSPAQHAIALWLMSRTGAEPVGARAFF
                     GGQPDATVAAAVARFSALRSDAQHAWLMAHFQDLRAGRIAIEDLP"
     gene            965..1564
                     /locus_tag="VFV93_02760"
     CDS             965..1564
                     /locus_tag="VFV93_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164292.1"
                     /translation="MRLWLLYLRSRFAFPALAGLLVIAGFGWAVGNRWGSQSDLFLIA
                     TVTVPLAAAVVICVTIDSPFGEVERSLSRPLVSMRFSQLAGLLIIAAVALAITATAWP
                     GDNVEWRFVRNLLGLTGVGLLFVRVLGGRLSWASPLGFATLALFQNGAAAGTAPGWAW
                     VVQPAPREAAAVIALVLLVSGLGLAARFGARVADGSDEH"
     gene            1652..2041
                     /locus_tag="VFV93_02765"
     CDS             1652..2041
                     /locus_tag="VFV93_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5164293.1"
                     /translation="MVLTRRSSKATTLYGYTVEEVARFLQYDKRAVRYWLRTGHLEGR
                     YDERLGDWRVTATELVAFLRQTSEPMPTGVRGDFSQPDEVLAEEIVFAAEGVEPITVD
                     LAPAATTRVSEEQPTRVRTRETVLPAC"
     gene            complement(2366..>2758)
                     /locus_tag="VFV93_02770"
     CDS             complement(2366..>2758)
                     /locus_tag="VFV93_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5164294.1"
                     /translation="LKTAAETAGEPPEGEWSAAQVLAHMAGIEQLWLERLNQLLRERE
                     PRLRPGGSGKAAEVQQQLMDGTVADNLDAFNTTRGEIVSLLMGLSLRDWERTGIHDSR
                     GEITIADVAETIIDHDAEHIAQIEALSA"
BASE COUNT          381 a          885 c          973 g          519 t
ORIGIN      
        1 tgctggccgg cgcgttgatc gttgtcatcg tcgatcgcga ggcgacctgg ggcgggctgg
       61 cggccgcgcc gctggtcgtt gggctgctgt cggtcgtggc cgggacggcg gtcggctacg
      121 ccggcggaac tgtcatcccg agccgcttcg ccgcgccgct gatggcggtc gtcttcgcca
      181 cagccgtgct gctggtgggg acgcgctcga cggcggtcgc cttcctctca ccattggcga
      241 tggatccgcg tgggtcaagc ccgtatgaca tcttctacca gccgccgacg attccgttgg
      301 cacagacagc gctctggctg gccggcgtgg ccgcctctgc gtgcgccgcg agtgtgctct
      361 ggcgtcggcg gacggcgctg acgtggggaa cgttccttgg cgcactggcc gttgcaacaa
      421 ccggggcggt cctggtgacc caggcgttcg ttcatccacc gtgggagcgg atctacgctg
      481 ggcagccgct ggcggactac gagctggtct gtgtcgagcg gtccatcccg gtttgtcttc
      541 atccagcata cgagtcccgg cttgaggaat acgccgacca gattgatgtt ctggtcgagc
      601 cgctggccgg cgtacccggt ggcccggttc gcgcggagca actgccgtcg agaaaaggcc
      661 cgcgactcga cgggacgctt gagattatcc cgagtggcct gattgttgcc caggctacct
      721 acgatctcgt gcgtgcgcct ggtggtgtct tcagtccggc acagcacgcc atcgcgctct
      781 ggctaatgtc tcgaactggc gcggagccgg tcggggcgcg tgcgttcttc ggcgggcagc
      841 cggacgcgac agtcgcggca gcagtcgcgc gtttctcggc gctcagatcg gacgcgcagc
      901 atgcatggct gatggcgcac ttccaggacc tgcgcgccgg ccggatcgcg attgaagatc
      961 tgccatgagg ctctggctgc tctacctgcg ttcgcggttc gcctttccgg cgctggccgg
     1021 gctgctggtg atcgcggggt tcggctgggc ggttgggaac cgttggggaa gccagtccga
     1081 tctgttcctg atcgcgaccg tcacggtgcc gctggcggcg gccgtcgtga tctgcgtgac
     1141 gatcgactcg ccgtttggcg aggtggagcg gtcgctcagc cgtccactgg tgtcaatgcg
     1201 cttcagccag ctggcgggct tgctgatcat cgcggccgtt gcgctggcga ttacggcgac
     1261 ggcctggccc ggggacaatg tcgagtggcg gttcgtgcgc aacctgctcg ggctgaccgg
     1321 ggtcgggttg ctgttcgtgc gcgtgctggg cgggcggctg tcgtgggcgt cgccgctcgg
     1381 gttcgcgacg ctggcgctct tccagaacgg cgcggccgcc ggtaccgccc ctggctgggc
     1441 gtgggtcgtt caaccagccc cgcgtgaggc agcggcggtc atcgcgctcg tcctgctagt
     1501 cagcggactc gggctggcgg cccgtttcgg cgcccgcgtt gcagacggct ccgacgaaca
     1561 ctgactcctc ggatccgacg tgacaggtag tcaaccgtgt gctatcctca gtgtacgtac
     1621 actctgaacg aggaacgtgc tgggggtagc gatggttctg acacgacggt cgagcaaggc
     1681 gacaacgctg tatgggtaca cggtcgagga ggtcgcgcgg ttcctgcaat acgacaagcg
     1741 ggccgtgcgt tactggctgc gaaccgggca cctggagggc cgctacgatg agcggctggg
     1801 cgactggcgg gtgacagcga cggagctggt tgccttcctg cgccagacga gcgagccaat
     1861 gccgaccggc gtgcgtggtg atttcagcca gcccgacgag gtgctggccg aggagatcgt
     1921 cttcgccgct gaaggagtcg agccgatcac tgtcgatctg gcgccggcgg ccacaacccg
     1981 cgtttcggaa gagcagccaa cccgcgtacg aacgcgggaa actgtccttc cggcctgttg
     2041 acgcactaga acatctgtgc tactctgagt tcatcgctgg agagggtgat ccgccgctcc
     2101 aggaaaggga gaagggcccg ttcgcgggag ctctttgacg gtcgaccacc ggcaaggttg
     2161 accgatccag gccgacatcg gcacgatccc cgacccggaa tccggcgcct cccttggctg
     2221 gcggtgcccc ttacaccccc ggtcgattcg gccccacacc ggacgaccag cacccccaat
     2281 ccctccccac cccaagcgga ccgtgcctcc ctgcaatatg gcacggtccg cgtcttttcc
     2341 ttcgtgatgc gtgatgcgtg acgcgctacg ccgaaagcgc ctcgatctgg gcgatgtgtt
     2401 cggcgtcgtg gtcgatgatg gtttcggcga cgtcggcgat ggtgatctca ccgcgcgagt
     2461 cgtggatgcc ggtccgttcc cagtcgcgca aagagaggcc catcaggagc gagacgatct
     2521 cgccgcgggt ggtgttgaag gcgtcgaggt tatcggcaac ggtgccgtcc atcagctgct
     2581 gctggacctc agcggccttg cccgacccgc cggggcggag gcgtggctcc cgctcgcgga
     2641 gcaactggtt gagccgttcg agccagagct gctcgatccc ggccatgtgg gccagcacct
     2701 gggcggcgct ccactcaccc tccggcggct cgcccgccgt ctcagcggca gtcttcaa
//
LOCUS       DASUGJ010000124         5481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_294245, whole genome shotgun sequence.
ACCESSION   DASUGJ010000124 DASUGJ010000000
VERSION     DASUGJ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294245"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFV93_02775"
     CDS             <1..967
                     /locus_tag="VFV93_02775"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5164295.1"
                     /translation="VSDANLVLGYLNPDYFLGGSIPLRADLARAAIARLGDELGLSVE
                     ETAWGIHRVVNENMAAAARVHIIERNRDPRALTTIAFGGAGPAHAVAVARILGSPAVI
                     FPPGAGVASAFGALVAPLSFTAGRTLMTRLDRADWDEIGAIYDAMRDEAFHELSQAGV
                     DPATIQTRRWAEMRLEGQYHEIAVDLTDGPLGPASIPNIQTAFDAAYARQYGRVLEGL
                     PIEVLHWRMSATGPEQQVALRRHERQGSDAATAIKAHRDVWFPGGYRQTTIYDRDKLA
                     SGMTFDGPAIIEEREATAVIWPGDRASVDEYLAIIVEIGRQEQPA"
     gene            964..2646
                     /locus_tag="VFV93_02780"
     CDS             964..2646
                     /locus_tag="VFV93_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5164296.1"
                     /translation="MTLTERRPAEMHDQFDPVSLEIMWSRLISVADEMWTTVLRTAVS
                     TIIGAAQDFGCELLDADGNSLAHSYRSMPVFNLIMPELTRKLIEAHPVATMREGDIYT
                     TNDPWLCAGHLDDIAVITPIFRNGRVVAFANTVAHTSSIGGALDGLAVRDLHEEGFFM
                     PLLKLYDEGRPNETLFALIRSNVRQPDMVLTDIESQVTANVVASQRVLAFMDEYQLDT
                     LSTLATAVQTRAEAAMRAAIEAIPDGRYEAEEMVEGTGNPLRLAVRIDVTGSDIHVDY
                     RGTDPQRLSGGINCTFTYTRAHTVYPLKCLLTPNVPNNEGCFRPIHVAAPERSVLNAL
                     PPASVNSRTLTGWHLHTLIFRALAPAMPERVQAGNGLMYTIRAYARDADGSPRNAHVI
                     CGGGRGAGYGHDSTTRNCFPSSAGNVPIEIFESRVPVIVEEDQLEPDSGGSGQWRGAP
                     GQRVTLRRHPQHELPVTLYVHPDRLRYPAPGLAGGGDSLHNNLLLNDQSLVRDGHLTS
                     GEIVLRTATDRFTSIVAGGAGYGDPMDRDPNAAERDCEFGYVERTGSDNLHQ"
     gene            2729..5062
                     /locus_tag="VFV93_02785"
     CDS             2729..5062
                     /locus_tag="VFV93_02785"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5164297.1"
                     /translation="MSRVPHDDRSGRIPRPLTPLIGRETVLDAICDLLRRPDTRLVTL
                     IGPGGIGKTRLALAVAERLRGEWRDGVVYVGLQSISDYEHVASAIAQAFGVRESGDES
                     IADGLWAALEPQQLLLVLDNFEQVLPAATLIGETLERCPQVRFLITSRAMLHLYGEYD
                     VPIPTLELPDLQHLPDVESLSKSEAIRLFVERSQAVSPAFTLSERNGPVVAAICARVD
                     NLPLAIELAAARTRLLSPPDLLSRLERRMPVLTGGPRNQPSRQQTMRDTIAWSYDLLP
                     AEEQRLFRQLAVFAGGWTLEAAEVVCGHDENVLDSLTTLVDHSLVRWDEQANGSTRFG
                     LLETIREFGLGQLEALGESVSVRDRHARWCSALAHEAVTHYSSHTEPEWHRRLDLELH
                     NLRAALMWLLKQGEAEPGLRLVADLGSFWFAGGYLSEGMAQADAFLAMPSAEPRSSAR
                     GRALNAAGFLAVWAGDFERAYDYAVEAVAILEERENLAGLPAALLTRGIVARYQGDVD
                     AAKTYTNRSVALAREVGDTLVLTRALSIVAAIEYQPGDKESRQRAEVALQEGLQVARE
                     SGNPSNAALILIRLARFAQHANEFDQAAEQYREAIALYHQAGYVSMIPESLEGLAVVV
                     AWQGYAPLATRMFGAAAALRSRIRAPVQAILQLDNEPAIAAARAALGEEAFASYWQSG
                     SALTMEDAVAEALASPEPLTASEQAEISTTPGHPLTTRELEVVKGLVDGLSNAEIASV
                     LFISPNTVSNHVSNIMNKLGLDSRTAIATWAVRHGVG"
     gene            5218..>5481
                     /locus_tag="VFV93_02790"
     CDS             5218..>5481
                     /locus_tag="VFV93_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164298.1"
                     /translation="MQRTETARRFEHGRITKRTLVIAAIGMLVLGLAAVALTVELPAR
                     GDTESREDTVPQITSDELYATEAAAAVAQVDGWQPTVGQLTSDE"
BASE COUNT          951 a         1847 c         1777 g          906 t
ORIGIN      
        1 cgtctcggac gccaacctcg tcctcggcta cctgaaccct gactacttcc tcggcggctc
       61 gattccgcta cgcgccgatc tggcgcgagc ggcgattgcc cggctcggtg acgaactggg
      121 cctctcggtc gaagagaccg cctggggcat tcatcgcgtc gtcaacgaga acatggcggc
      181 cgctgcacgg gtccacatca tcgagcgcaa tcgcgacccg cgcgcgttga cgacgatcgc
      241 cttcggtggc gctggtccgg cccacgccgt cgccgtcgcc cggattctcg gcagtccggc
      301 ggtgatcttc ccgcctggcg ccggtgtcgc ttccgccttc ggcgcactgg tcgccccgct
      361 ctcgttcacg gccggccgga cgctgatgac ccgtctcgac cgggccgatt gggacgagat
      421 tggcgccatc tacgacgcga tgcgggacga ggcgttccac gagctgtcgc aggccggcgt
      481 cgatccagcg acgatccaga cccgccgctg ggccgagatg cggctggagg gccagtacca
      541 cgagatcgcc gtcgatctta ccgatggacc gctcggtccc gcttcgatcc caaacatcca
      601 gaccgccttc gacgccgcct acgcacgcca gtatggccgc gtcctcgaag ggctgccgat
      661 cgaggttctc cactggagga tgagcgcgac tggacccgag cagcaggtgg cgctgcgtcg
      721 ccacgagcgg cagggcagcg atgccgccac cgcgatcaag gcccatcgcg atgtctggtt
      781 ccccggcggc tatcgtcaga ccaccatcta cgatcgtgac aagctcgcgt cgggcatgac
      841 cttcgacggc ccggctatca tcgaggagcg cgaggctacc gccgtcatct ggcccggcga
      901 ccgcgccagc gtggacgaat acctcgccat catcgttgag attggcagac aggagcagcc
      961 ggcatgacac tgacagaacg ccgcccggcc gaaatgcatg accagttcga cccagtctcg
     1021 ctggagatca tgtggagccg tctgatcagc gtcgccgacg agatgtggac gaccgtcctc
     1081 cgaacggccg tctcgaccat catcggcgct gcccaggact tcggttgcga actgctcgac
     1141 gcggacggta actcgctggc ccactcctac cgctcgatgc cggtcttcaa cctgatcatg
     1201 ccggaactga cccgcaagct gatcgaagct catccggtcg caacgatgcg cgagggcgac
     1261 atctacacga ccaacgaccc ctggctctgc gccggccatc tggacgacat cgccgtcatc
     1321 acgccgatct tccgcaacgg ccgtgtcgtc gctttcgcca acaccgtcgc gcacacctcg
     1381 tcgatcggcg gcgcgctgga cgggctggcc gttcgcgatc tgcacgagga gggcttcttt
     1441 atgccgctcc tcaagctcta cgatgagggc cggccgaacg agacgctctt cgcgctgatc
     1501 cgttccaacg tccgccagcc ggacatggtc ctgaccgaca tcgaatcgca ggtgacggcc
     1561 aacgtcgtcg cgagtcagcg cgtgctggcg tttatggacg agtaccagct cgacacgctc
     1621 tcgacgctgg cgacagccgt tcagactcgt gccgaggccg ccatgcgggc cgcgattgag
     1681 gcgatccccg acggccgcta cgaggccgag gagatggtcg agggtaccgg caacccactc
     1741 cggctggcgg ttcggatcga tgtcaccgga tccgacatcc acgtcgatta ccgcggcacc
     1801 gacccacagc gcctctcagg cggcatcaac tgcaccttca cctacacccg cgcacatact
     1861 gtctacccgc tgaagtgcct gctgacgccg aacgtcccca acaacgaggg ctgcttccgg
     1921 ccgatccacg tcgccgcacc ggagcgctcg gtcctgaacg ccctgccgcc tgcctcggtc
     1981 aactcacgga cattgaccgg ctggcacctg cacacgctca tcttccgcgc gctcgcgcca
     2041 gccatgcccg aacgggtcca ggccggcaac ggcctgatgt acacgatccg cgcctacgcc
     2101 cgcgacgccg acggcagccc gcgcaacgcg cacgtcatct gcggcggcgg ccggggcgcc
     2161 ggctacggcc acgacagcac gacccgcaat tgctttccgt ccagcgccgg caacgtcccg
     2221 atcgagatct tcgaatcccg cgtcccggtc atcgttgagg aagatcagct cgaaccggat
     2281 tcaggcggca gcggccaatg gcgcggcgcg ccaggccagc gcgtcaccct ccgccgccac
     2341 ccgcagcacg agctgcccgt taccctctac gtccaccccg accggctacg ttaccccgcg
     2401 ccgggcctgg ccggcggcgg cgactcgctc cacaacaacc tgctgctcaa cgaccagtcg
     2461 ctcgtccgcg acggccacct caccagcggc gagatcgttc tccgcaccgc caccgaccga
     2521 ttcacgtcga tcgtcgccgg cggtgcaggc tacggcgatc caatggatcg cgatccgaac
     2581 gccgccgaac gtgactgcga gttcgggtac gtcgagcgta caggctccga caacctccac
     2641 cagtaggtcc ggctcgcggt tgccttgaca acaatcgatt gccctatcat tatcgtgaca
     2701 tcacacataa tcgttggggc aaccggtcat gtccagggtt ccgcacgacg atcgatcagg
     2761 ccgcattccg cgaccactca cgccgctcat cggacgtgag acggtgctcg acgcgatctg
     2821 cgacctgctg cgccggccag acacgcggct ggtgacgctg atcggtccgg gtgggatcgg
     2881 caagacgcgg ctggcgctgg cggtcgccga gcggttgcga ggtgagtggc gtgacggcgt
     2941 cgtctacgtg ggattgcagt cgatcagcga ttacgagcat gtggcgtcgg cgatcgccca
     3001 ggctttcggc gtccgcgaat cgggcgacga atcgatcgcg gatggtctgt gggcggcgct
     3061 tgagccacag cagctgctgc tcgtgctgga caacttcgag caggtgttgc cggccgcgac
     3121 attgatcggc gagacgctgg agcggtgtcc gcaggtgagg ttcctcatca ccagccgggc
     3181 gatgttgcac ctttacggcg agtacgatgt gccgatcccg acactggaac tccccgacct
     3241 acagcatttg cccgatgtcg aaagcctgtc caaatctgag gcaatccgat tgttcgtgga
     3301 gcggtcgcag gccgtgtcgc cggccttcac gctgtcggag aggaatgggc cggttgttgc
     3361 cgcaatctgc gccagggtcg acaacctgcc gctggcgatc gagttggcgg cggcgcggac
     3421 gcgtctgctg tcgccgccag acctgctatc gcggctggag cggcggatgc ccgtcctgac
     3481 cggcggtccg cgcaaccagc cgagtcgtca gcagacgatg cgcgacacga tcgcctggag
     3541 ctacgacctg ctgccggccg aggagcagcg gctgtttcgc cagttggcgg tctttgccgg
     3601 cggctggaca ctggaggccg ccgaggtcgt ctgcggccac gacgagaatg tccttgatag
     3661 tctgacgacg ctcgttgacc acagcctcgt ccggtgggac gaacaagcga atggctcgac
     3721 gcgcttcggc ctgctggaga cgatccgcga gttcggactc ggacaactgg aggcgctcgg
     3781 agagtccgtg tcggttcgcg atcgccatgc gagatggtgt tcggcgcttg cgcacgaagc
     3841 cgtgacgcat tacagctcgc acacggaacc cgaatggcat cgccgcctcg acctggaact
     3901 gcacaacctg cgcgcggcgc tgatgtggct cctcaagcag ggcgaggccg aaccgggact
     3961 gcggctggtc gcggatctgg gcagcttctg gtttgccggc ggatacctgt cggaaggcat
     4021 ggcccaggcg gatgcattcc tggcgatgcc gagcgcggag ccgaggtcgt cggcacgcgg
     4081 tcgcgcgctc aacgctgccg gctttctggc agtctgggcc ggcgacttcg agagggcgta
     4141 cgactatgca gtcgaagctg tcgccatcct tgaggagcgg gagaatctgg cagggcttcc
     4201 tgccgcgctg ctgacgcgcg ggatagtggc gcggtaccag ggggacgttg acgccgcgaa
     4261 gacgtacacc aatcgcagcg tcgctctggc gcgcgaagta ggggacaccc tggtgttgac
     4321 gcgagcgctc agcatcgttg ccgcgatcga gtaccagccg ggggacaagg aaagcaggca
     4381 acgggctgaa gtggcgcttc aggaaggcct tcaggtcgcc cgcgagagcg gcaatccgag
     4441 caacgcagcg ctgatcctga ttcgactggc caggtttgcc cagcacgcga acgagttcga
     4501 ccaggcagcg gagcagtacc gcgaggccat cgcgctctac catcaagccg gatacgtctc
     4561 gatgattccc gaatctctgg aggggctggc tgtcgtcgtc gcctggcaag ggtacgcgcc
     4621 tctcgcgacg cgaatgttcg gggctgcggc cgccctgcgc tcgcggattc gggcgcctgt
     4681 ccaggcgatc ctccaactgg acaacgagcc ggccatcgcg gccgcacgcg ctgccctcgg
     4741 cgaggaggct tttgcgtcgt actggcaatc cggcagcgcg ttgacgatgg aggacgcggt
     4801 tgccgaagca ctggcctccc cggagccact caccgcctcc gagcaggcag agatctccac
     4861 gacgccgggt cacccgctca cgacgcgtga gttggaggtc gtcaaaggcc tcgtcgatgg
     4921 tctgtccaac gccgagatcg ccagcgtgct gttcatcagc ccgaacacag tcagcaacca
     4981 tgtctccaac atcatgaaca agctggggct ggattcgcga acggccatcg cgacctgggc
     5041 ggtccggcac ggcgtcggct gacggcaggc cggcggcata gtaagcaatt actagtcact
     5101 cgcgagacgc gaatagtgaa gccgtagggt gtgcgcgcgc cttcgcaagc acatgctgtg
     5161 gatgtggttg acagtcggag atcagccatc agacagcaga ctcgaaggag cgctgatatg
     5221 caacggactg agacggctcg acgatttgaa cacgggcgga tcacgaagcg gacgctggtc
     5281 atcgcagcca tcggcatgct ggttctcggg ctggctgcgg tcgcgctgac cgtcgagctg
     5341 cctgcgaggg gcgacaccga gtcgcgagag gacactgtcc cgcagattac ctcggatgag
     5401 ctgtacgcga cggaagctgc cgctgcagtc gcgcaggtag atggctggca accgacggtt
     5461 gggcaattga cctcggacga g
//
LOCUS       DASUGJ010000125         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_711030, whole genome shotgun sequence.
ACCESSION   DASUGJ010000125 DASUGJ010000000
VERSION     DASUGJ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711030"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VFV93_02795"
     CDS             complement(<1..1082)
                     /locus_tag="VFV93_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164299.1"
                     /translation="MQAWLGKRIYYGWVVVGVTAFVLLVAAGVRAGSTVLITPLEDDL
                     GWSRSSISFAIMVSLILYGLAGPIGGTLMDRYGPKRLMLAGIVLTALSSIVTALSATL
                     WQFTAVWGVAGGLGTGVVASVLGAAVANRWFVRRRGLAIGIFGASSSAGQMIFVPLLI
                     WVFNLTDWRGGVVVLGLTTATLLAPVLLLMRDAPSDLGLRPLGGTDEPSAPQMARAGS
                     VMRGAIRVPEFWLLSGSFFICGATSIGLISTHFLPYSSDEGIPEATAAILLAIMGGMN
                     FVGTLASGWLTDRYDPRKLLAGYFVIRALSLFMLPFVANTPGLAVFAIFFGLDNIATV
                     PPTVSLTADIFGRRNIGTVFGWIFAA"
     gene            complement(1207..1623)
                     /locus_tag="VFV93_02800"
     CDS             complement(1207..1623)
                     /locus_tag="VFV93_02800"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5164300.1"
                     /translation="MSVEANKALIRRYFETWNANDVDALDDIIAADAIDHMAYEGQQA
                     GRAGYRDFYATWHSAFPGFRAEIEDMIAEGDRVATRWTFRGRHLGEYNGIAPTGREIV
                     YTAVSIQRIADGRVAEEWYVGDTYEFMRQLTDDDRS"
     gene            complement(1688..2794)
                     /locus_tag="VFV93_02805"
     CDS             complement(1688..2794)
                     /locus_tag="VFV93_02805"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5164301.1"
                     /translation="MSEAQQGFDTTGKLAPLQALLDHFITEGQLDGTAVAVAQGGKQV
                     AELYAGNAAPGRPAGADTLWPLASISKLYTAAVVMALVEQGVLTLSMTARSVLPEFDG
                     GGREIVTLRHLLTHTSGVVYESPEMEQRLLDQLTIDEIIDEAYTYELLFTPGTRHSYS
                     DYNYALLGRIAAITAGKPFPDLVRELVLEPGKLGDTFMPPPRSEYQRLAHVVEPLAYR
                     TESDMYNSAYALDLAHPAFGTVATAGDLLRFGLLFAPNGAHRIHSTATIQAMTTDQTG
                     GNAPGTMFGNEADQPRPWGLGFMVKGTTGFGADLLSPATFGHGGASGCVLQVDPVTDI
                     AIAYVSNKHARTGREPFTKRQISVCNVVAAALTR"
     gene            complement(2859..>3099)
                     /locus_tag="VFV93_02810"
     CDS             complement(2859..>3099)
                     /locus_tag="VFV93_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164302.1"
                     /translation="AAYFVAINLIGITILLAGGTVGQTELAISAALTPAALVGTLIGN
                     RLVRRFTAAQFRRLTLGLLLLTGLMGVATAVAALV"
BASE COUNT          549 a         1041 c         1026 g          483 t
ORIGIN      
        1 tgggccgcga agatccagcc gaagaccgtc ccgatgttgc gccggccgaa gatgtcggcc
       61 gtcaggctga ccgtcggcgg gaccgtcgcg atgttgtcga ggccgaagaa gatggcaaac
      121 accgccaggc ccggcgtgtt ggcgacgaac ggcagcatga acagcgacag cgcccggatg
      181 acgaagtagc cggccagcaa cttgcgcggg tcgtagcgat cggtcagcca gccggaggcg
      241 agcgttccga cgaagttcat gccgcccatg atcgccagca ggatcgccgc cgtcgcctcg
      301 ggaatccctt cgtccgatga gtacggcaga aagtgggtgc tgatcaggcc gatcgaggtc
      361 gcgccacaga tgaagaaact cccggatagc agccagaact ccggcacccg gatcgccccg
      421 cgcatgacgc tgccggcgcg ggccatctgc ggcgccgacg gctcgtcggt cccgcccagt
      481 ggccgcaacc cgaggtcgga tggcgcgtcg cgcatcagca gcaggaccgg cgcgagcagc
      541 gtcgccgtcg tcagaccgag cacaaccacc ccgccgcgcc agtcagtcag gttgaagacc
      601 cagatcagca gcgggacgaa gatcatctgc ccggccgaag aggacgcgcc gaagatgccg
      661 atcgccagcc cgcgccgccg cacgaaccag cggttggcga ctgccgcgcc gagcacggac
      721 gcgacaacgc ccgtccccag cccgccggcg acaccccaga cggccgtgaa ctgccagagc
      781 gtcgccgaca gcgccgtcac gatcgacgag agcgccgtca gcacgatccc ggccagcatc
      841 agccgcttcg ggccgtagcg gtccatcagt gtcccgccga tcggcccggc cagaccatac
      901 aggatcaggc tgaccatgat cgcgaacgag atcgacgacc ggctccagcc gagatcatcc
      961 tccagcggcg tgatcagcac ggtagaaccc gcccggacac cggccgcgac cagcagcacg
     1021 aacgccgtca caccgacgac gacccagccg taatagattc gcttcccaag ccacgcctgc
     1081 atgctgctcc agtgatgatg cccaggttcc agatgccggt tcgcagcatg gcatccgaat
     1141 ggccggtcgg caacatccaa tatcgtgctg gccccagagc cacttcaatc gctgaaggag
     1201 cgcgacctac gacctgtcat catcggttag ctgccgcatg aactcgtagg tatcaccgac
     1261 ataccactcc tcggcgacgc gtccatcggc gatgcgctgg atcgagacgg cggtgtagac
     1321 gatctcgcgg ccagttggcg cgatgccgtt gtattcgccg aggtggcgac cacgaaaggt
     1381 ccagcgggtc gcaacacgat ccccctcagc gatcatgtct tcgatctcgg cccggaagcc
     1441 ggggaatgcg ctgtgccagg ttgcgtagaa gtcgcggtaa cccgcgcgcc ccgcctgctg
     1501 gccctcgtac gccatatggt cgatcgcgtc ggcggcgatg atgtcgtcga gcgcatctac
     1561 atcgttggcg ttccacgttt cgaaataccg ccggatcagc gccttattgg cctcgacgga
     1621 catcggggtt cctttctgtt ctgagtgacg cgtaacgcgt cacgcatcac gcgtcactca
     1681 gcacccgtca cctcgtcaat gctgccgcga cgacgttgca gacgctgatc tggcgtttgg
     1741 tgaacggctc gcggccggtg cgggcgtgct tgttggagac gtaggcgatg gcgatgtcgg
     1801 tgacggggtc gacctgcagg acgcagccgc tggcgccgcc gtggccgaag gtggccggcg
     1861 agagcaggtc ggcgccgaag ccggtcgtac ccttgaccat gaagccgagc ccccacggac
     1921 ggggctgatc ggcttcgttg ccgaacatcg tgccgggcgc attgccgccg gtctggtcgg
     1981 tcgtcatcgc ctggatcgtc gctgtcgagt ggatgcggtg agcgccgttt ggcgcgaaga
     2041 gcagaccgaa acgcagcaga tcgccggccg tcgcgacggt gccgaaggcg gggtgggcaa
     2101 ggtcgagcgc gtaggccgag ttgtacatgt cgctctcggt ccggtacgcc agcggctcga
     2161 cgacgtgcgc gagccgctgg tactcgctgc gcggcggcgg catgaaggtg tcgccgagct
     2221 tgcccggctc cagcaccagt tctcggacga ggtcggggaa cggcttgccg gccgtgatcg
     2281 cggcgatccg gcccagcagt gcgtagttgt agtcgctgta cgagtggcgt gtgccgggcg
     2341 tgaagagcaa ttcgtaagtg taggcctcgt cgatgatctc gtcgatcgtc agctgatcga
     2401 gcagccgttg ctccatctcc ggcgactcgt agacaacgcc ggacgtgtgc gtcagcaggt
     2461 ggcgcagcgt gacgatctcg cgaccgccac cgtcgaactc cggcaggacg ctgcgggccg
     2521 tcatcgagag ggtcagcacg ccctgctcga ccagcgccat cacgaccgct gccgtgtaga
     2581 gcttcgagat cgatgccagt ggccagagtg tgtctgcccc ggccggtcgc cccggcgctg
     2641 cattaccggc gtagagctcg gcgacctgtt tgccgccctg ggcgacggca acggccgtcc
     2701 cgtcaagttg cccctcggtg atgaagtggt ccagtagcgc ctgaagcggc gccagcttac
     2761 cggtcgtgtc gaacccctgt tgagcctcgc tcacgtcgta tcctcccttt gcgtcgtgtc
     2821 gctgccggaa ctatcgccgc aacgcgccaa ttcggcaatc acaccagcgc ggctaccgcc
     2881 gtcgcgacac ccatcagccc ggtcagcaac agcaggccga gcgtcagccg gcggaactgg
     2941 gccgccgtaa agcgccggac gagccggttg ccgatcagcg ttccgaccag cgcggccggt
     3001 gtcagtgccg ccgagatggc aagctcggtc tggccgaccg tgccgccggc cagcaggatc
     3061 gtgatcccga tcaggttgat cgcgacgaaa taggccgcg
//
LOCUS       DASUGJ010000126         9545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_16799, whole genome shotgun sequence.
ACCESSION   DASUGJ010000126 DASUGJ010000000
VERSION     DASUGJ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9545
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16799"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1547
                     /locus_tag="VFV93_02815"
     CDS             342..1547
                     /locus_tag="VFV93_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164303.1"
                     /translation="MDRVRNLLQHRALLVFAMGHFTVDMFGGLMPVLYALLSDEFQLS
                     NADIGLIALAYTSASSLSQPLFGYLADRFGSRYFAVASLIWSATMVGLAGLVPSYAML
                     ILVSMMAGLGSGAYHPQGATNAAAVSGEMRRNTAMSLYTVGGTSGYSLGPVLGALIFF
                     IFGRYGTLAVAPFGFAIALLMLRELRQLGLGVREHHATQRATQGKINWKPLLVVLSVV
                     MLRSWVSLSVITFIPVWFKEQGYSATFYSILATLILGCGAVGTLTGGVLADRIGQRAV
                     LSVSLICAAPALILFAAFPGPLSLLFGPLFGFFSDMGLSITLVMAQRLLPGRVGVASG
                     FILGMGFVTGGIGAPVTGAIADDIGTGPAIMLMAILIVAAAAIAARIPRVALQPARAA
                     PVPTPEPAT"
     gene            1544..1912
                     /locus_tag="VFV93_02820"
     CDS             1544..1912
                     /locus_tag="VFV93_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164304.1"
                     /translation="MKFGNVEGLCVPLPGDEPLVRVTVYLETDMGIDIVREIEAEPEP
                     REDGEPDLWWEADQWTQETIATILIPRGWELLGATDPPTRSPDEVPRSPRYALRTQLW
                     VPPPGWDEDEEDTGNVGANR"
     gene            complement(2001..2624)
                     /gene="coaE"
                     /locus_tag="VFV93_02825"
     CDS             complement(2001..2624)
                     /gene="coaE"
                     /locus_tag="VFV93_02825"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX5164305.1"
                     /translation="MRQRKHVIGLTGNIATGKSTVVRMLEELGAEAIDGDALVHRLMG
                     PGSPLAEQIRAEFGEQTVNPDGSINRPELGKVVFSDPAKLAALESITWPPVLAAKREA
                     IDSPGPDVLILDAIKLWESGMADDCDEVWVVSADREQQIARIIARNKVDRAEAERRID
                     AQPPQSEKIAKADVVIDNSGTLDETREQVLREWRRLTIEDDNVGANR"
     gene            complement(2749..4773)
                     /locus_tag="VFV93_02830"
     CDS             complement(2749..4773)
                     /locus_tag="VFV93_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX5164306.1"
                     /translation="MATTILTVQDVSKRFITDVIFSGVTFQVNEREHVALVGTNGAGK
                     STLLKIIAGVEEPTEGGIVYQSGLRVAYQAQEATFTDDRTLYEETIEAFAHVRAIGER
                     MATLEQQMAGVDGDALDAMFEEYARLSTEFETHGGYEMEHRTEAVLHGLGFPEEMFSD
                     PVKQLSGGQKTRVALAKALLSEPDLLMLDEPTNHLDLEAIEWLENFMRGWNRAFIVVS
                     HDRYFLDRVTDRTLDMAFGKLEDYPGGYNRYLKLREERMARRLKEYEEQQEFIARTEE
                     FIRKYKAGQRSKEARGRETRLARLERLERPQEHDQLHLRMRADLRSSRNVLVVKPITV
                     GYKPRPEMGETEQDVFVQTDKELTIERGDRVALLGPNGSGKTTTLRTFVGQLDALKGR
                     VEFGTNVKVAYYQQGHEGLDPKMSALETILHDQNMGEETARNLLGRFLFSGDDAYKPV
                     SALSGGERSRLALARLTLARANFLVLDEPTNHLDISAREVLEDVLDGYDGTMLFVSHD
                     RYFVDRIANRIWAIEDGTIRTYLGNYSDMQRQRARQAAEGAAPPAAPPRIPAATVHTH
                     ETEPNGAVQPVPKPAPVNPGKQTRVNERALRDAQKRLNAAEREVSKLEEKLNTLSDEL
                     TRASIDGNVERVSKLGTDYETMQQQLEAAYTRWNEATAELDALQVEIAGV"
     gene            4984..6252
                     /locus_tag="VFV93_02835"
     CDS             4984..6252
                     /locus_tag="VFV93_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX5164307.1"
                     /translation="MGGTQARGNAGGMGRSDSEGAGVVVACVFIPHFPLRVEILRHPE
                     LDGLPLALTDLAGAGRRRIVECSPEATERGLRVGMPLREAVGTCPQAVILTSDPVAYA
                     NAFADLLRALGEVSPAVEAAEAGVAFVDLRGLDRLYGGSGNITAALLRAVPPALRPRV
                     GLAANKFTAHVAATQASPGGVRMIPAAYAKAFLAGSPVDVLPVPLEMRRRLERFGLYH
                     LRDIARLPLGKLQAQFGPDGRRVWELAHGQDSAPIRPLAQLERFVERLPLPSPTVQIE
                     TVLMALRQACERAYTRKEVRGRGVRQVRLQLLLEEHRSWERTVTLKGTIDDPWTLNTT
                     LRYRLQGLQLEGAVEEVVLELIGLTDIHARQTQLFDELDLRRRQRREIGEAVRQLKQR
                     YGGSPLYHVVGIEPWSRIPERRLGLFAWEA"
     gene            6325..6588
                     /locus_tag="VFV93_02840"
     CDS             6325..6588
                     /locus_tag="VFV93_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HEX5164308.1"
                     /translation="MKTATVELPEDILALLAQTRLGDRTQSDQVRIAVAIHLFQEGII
                     SIGKAAELAGEPRIGFERFLAGIGIAPVQYDEEMYAQDLRGIA"
     gene            complement(6585..7037)
                     /locus_tag="VFV93_02845"
     CDS             complement(6585..7037)
                     /locus_tag="VFV93_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEX5164309.1"
                     /translation="MVDARWRVPLDTNIWISAFINPVGPPAHVLDLYTEGHYQLVVSQ
                     PLLDEISDVLSRPRVRRAVRFRDDWMTLWLRRLQREAVLVAPKGNLQLCRDPKDDIVL
                     ETAILGEADYLVSRDDDIKRDLNLIAHLREHNIAVVTVAQFLKLLGDN"
     gene            complement(7030..7368)
                     /locus_tag="VFV93_02850"
     CDS             complement(7030..7368)
                     /locus_tag="VFV93_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX5164310.1"
                     /translation="MKRTNIYLDENLDRLIRYLAVQQGRSFTDIVREALNDYAKAQGY
                     PDPSRIRMPLPMTKEEREEWRVEMKQLLQEIRESVETNLTPDEIEDLITAASNEARQV
                     RLSEHLTAHG"
     gene            7430..7717
                     /locus_tag="VFV93_02855"
     CDS             7430..7717
                     /locus_tag="VFV93_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164311.1"
                     /translation="MFWKSPEPVNEAETIRLLNQPRPLIVEADDRGVPRRVGAGERQR
                     RVVRIVDAWRIDDEWWRTTISRRYLLVMLEDGVIRTIFQDLIGNGWYEQTY"
     gene            7833..>9545
                     /locus_tag="VFV93_02860"
     CDS             7833..>9545
                     /locus_tag="VFV93_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5164312.1"
                     /translation="MYAELHLHTAFSFHDGASMPAELVGRAVELGYEALAITDHNGLH
                     GAMEFAQAAKTAGIQAIIGAELTLLDGSHLTLLAETAAGYANLSQVITRAHLDSERLD
                     PRLALETLREHTRGLILLTGCRNGELALLVDAGRMDAARSVLDRYRDWFGPENVFVEL
                     QQNMVYGDTRRLATLARLAEEAGLGIVATGNVHYHVRERHRLQDVLVAIRHRLTLDAS
                     HQVRRANSEFFLHPQSEMERRFRHYPEAIRNTLVIANRCAGFDMTRDLRYRFPDYERS
                     LDHSPAATASPPMSPDDQLASDCYALARERYSADAEEYARALTRLEQELGLVKRNNLA
                     WFFLVYRDIMILAKQIAAEVRGTGARGAYALPPGRGRGSSVSSIICYLIGLSHIDPLR
                     HNLSVGRFLNESLASAPDIDIDFPRDIREKLIARVYEVYGNDHAALVCAFPTYHIRSA
                     IRDIGKTLGLPAPDLDRLAKLSERTSSDRLAAEMERMPEFRDRLDAPLWRELIELAGQ
                     ISRFPRHVSQHSGGMVISAQPLTELVPVQPAAMEGRYICQWDKDSCDDASFVKIDFLA
                     LGMLS"
BASE COUNT         1494 a         3134 c         3096 g         1821 t
ORIGIN      
        1 atgcgccgaa gcgaccgctc ccctgcgtcc ggttacggcg ggacgatgcg cgagtcctcg
       61 gttcggccag gtcatagccc ctcctcattc ccctcgctct gctgcattgt ggtgagttgg
      121 atggatgttg cgacgtatgt gcagttctcc ggcggcatac ccgagactgc gactgatttc
      181 aggtgtgaac gcatactaac ggcaaagaat tgacaggtca aggaccgcgc gaaccgcgcg
      241 agcaatcatc ccgtcacgag ttgcgggtgc gcactgtcga atcaccccgt atcatggtgg
      301 atcgttgcgc cggcccggac gggaagcgat agggttcgtc ggtggatcga gttcggaatc
      361 tgctccagca tcgcgccctg ctggtcttcg cgatgggcca cttcacggtc gacatgttcg
      421 gcgggctgat gcccgtcctc tacgcgctgc tctcggacga gttccagctc agtaatgcgg
      481 acatcggcct gatcgcgctg gcctacacct cggcgtcgtc gctgtcgcaa ccgctgttcg
      541 gctacctggc cgatcgcttc ggtagccgct acttcgccgt cgccagcctg atctggtcgg
      601 cgacgatggt tgggctggcc ggactggtgc cgtcctacgc gatgctgatc ctcgtctcga
      661 tgatggccgg cctcggctcg ggcgcgtatc acccgcaggg tgcgacaaac gctgccgccg
      721 tctccggcga gatgcggcgt aacacggcca tgtcgctcta caccgtcggc ggcaccagcg
      781 gctactcgct cggcccggtc ctcggcgcgc tgatcttctt catcttcggt cgctacggca
      841 cgctggccgt cgccccgttc ggcttcgcga tcgcgctgct gatgctgcgg gaactgcgcc
      901 agctcggcct cggcgtccgc gaacatcacg ccacccagcg cgcaacgcag ggcaagatca
      961 actggaagcc gctgctggtc gtcctctcgg tcgtcatgct ccgctcgtgg gtgtcgctct
     1021 cggtcatcac cttcatcccc gtctggttca aggagcaggg ctactcggcc accttctaca
     1081 gcatcctggc gacgctcatc ctcggctgcg gcgcggtcgg gacgctcacc ggcggcgttc
     1141 tcgccgaccg gatcggccag cgtgcggtcc tgtccgtctc gctgatctgc gcggccccgg
     1201 ccctgatctt gttcgccgcc ttccccggtc cgctctcgct gctgttcggc ccgctgttcg
     1261 gcttcttctc cgatatgggt ctctcgatca cgctcgtgat ggctcagcga ctgctgccgg
     1321 gccgggtcgg cgtggcgtcc ggcttcatcc tcggcatggg cttcgttacc ggcgggatcg
     1381 gcgcacccgt gacgggcgcc atcgccgacg acatcggcac cggcccggcc atcatgctga
     1441 tggcgattct gatcgtcgcc gctgccgcga tcgccgcccg catcccccgc gtcgcgctgc
     1501 agccggcccg ggcggccccc gtacctacgc cggagccggc cacatgaagt ttggcaatgt
     1561 cgaaggactc tgcgtcccgc tgcccggcga cgagccgctt gtccgcgtca ccgtctatct
     1621 tgaaaccgac atgggaatcg acatcgttcg cgagatcgaa gccgagccgg aaccgcgcga
     1681 ggacggcgag cccgacctct ggtgggaagc cgaccagtgg acccaggaga cgatcgcaac
     1741 gatcctcatc ccccgcggct gggaactcct cggtgcgacc gacccaccaa cccgctcacc
     1801 cgacgaggtc ccccgctcgc cccgctacgc tctccggacg cagctctggg ttccaccgcc
     1861 cggctgggac gaggacgagg aagacactgg caatgtaggg gcgaatcggt gattcgcccg
     1921 gcaattcgat cggcgttcag ccgatcacaa tcccaattgg cgcattgggg ccgctgaacg
     1981 cggccagatt gtcgggcgaa tcaccgattc gcccctacat tgtcgtcttc gatcgtcaat
     2041 cgacgccact cgcgcagcac ctgctcgcgc gtttcatcca gcgtgccgga gttgtcgatc
     2101 acgacatcgg ctttcgcgat cttctctgac tgcggcggct gggcgtcgat gcggcgttcc
     2161 gcttcggcgc ggtcgacctt gttgcgggcg atgatgcggg cgatctgttg ctcgcggtcg
     2221 gcgctgacga cccagacttc gtcgcaatcg tcggccatcc ccgactccca gagcttgatc
     2281 gcgtccagga tcagcacatc cggtcccggc gagtcgatcg cctcacgctt ggcggccagc
     2341 accggcggcc aggtgatcga ttccagcgct gccagcttgg ctggatcgga gaaaacgacc
     2401 tttcccaact cgggccggtt gatcgaaccg tccggattga cggtctgctc gccgaactcg
     2461 gcccgaatct gctccgccag cggcgatccc ggccccatca gccggtggac cagcgcgtcg
     2521 ccgtcaatcg cctccgcgcc cagttcttca agcatccgaa cgacggtgga cttcccggtc
     2581 gcgatgttgc cggtcaaccc aatgacatgc ttgcgttgtc tcacgaacct caccccccag
     2641 ccccctctcc gcgtcggaga ggggagtcag acagacgaac actagctccc cctctccgcg
     2701 ccggcggggt accctccggg tcgcgggctg gggggtgagg ttccgtcctc acacgcccgc
     2761 gatctcgacc tgtagcgcgt ccagttccgc cgtcgcttcg ttccagcgcg tgtaggcggc
     2821 ttcgagttgt tgctgcattg tctcgtagtc ggtgccgagc ttggagaccc gttcgacgtt
     2881 gccgtcgatg ctggcgcggg tcagttcgtc ggagagggtg ttgagcttct cctccagctt
     2941 gctgacctcg cgttcggccg cgttgagccg cttctgggcg tcgcgcagcg cccgctcgtt
     3001 gacgcgcgtt tgcttgccgg gattaacggg cgccggcttc ggcacgggct ggacagcacc
     3061 gtttggctct gtctcgtgcg tgtgaacggt ggcagccggt atccgcggtg gcgcggccgg
     3121 cggcgctgcc ccctcggcgg cctggcgggc gcgctggcgc tgcatgtcgc tgtagttgcc
     3181 gaggtaggtc ctgatcgtac cgtcctcgat cgcccagatc cggttcgcga tccggtcgac
     3241 gaagtagcgg tcgtgcgaga cgaacagcat cgtcccgtcg tagccgtcca gcacatcctc
     3301 cagcacctcg cgggcgctga tgtcgaggtg gttggtcggc tcgtcgagga cgaggaagtt
     3361 ggccctcgcc agcgtcagcc gcgccagcgc cagccgtgaa cgctccccac cggagagggc
     3421 cgagaccggc ttgtaggcgt cgtcgccgga gaagaggaag cgccccagca ggttgcgcgc
     3481 cgtctcctcg cccatgttct gatcgtggag gatcgtctcc agcgcgctca tcttcgggtc
     3541 gagcccctcg tgcccctgct ggtagtaggc gaccttgacg ttcgtcccga actcgacccg
     3601 ccccttgagg gcgtcgagct ggccgacgaa cgtccgtagc gtcgtcgtct tgccggagcc
     3661 attgggaccg agcagcgcga cccggtcacc ccgctcgatc gtcagttcct tgtcggtctg
     3721 gacgaagaca tcctgctccg tctcgcccat ctccggccgt ggcttgtagc cgaccgtgat
     3781 cggcttgacg accagcacgt tccgcgatga gcgcaggtcg gcccgcatcc gcaggtggag
     3841 ctggtcgtgc tcctgcgggc gttccagccg ttccagccgc gccagccgcg tctcacggcc
     3901 gcgcgcctcc ttcgagcgct gccccgcctt gtacttgcgg atgaactcct cggtccgggc
     3961 gatgaactcc tgctgctcct cgtattcctt cagccgccgc gccatccgct cctcgcgcag
     4021 cttcaggtag cggttgtagc cgccggggta gtcctccagc ttgccgaagg ccatgtcgag
     4081 cgtccggtcc gtcacccggt cgaggaagta gcggtcgtgg gagacgacga tgaaggcccg
     4141 gttccagccg cgcatgaagt tttccagcca ctcgatggct tccagatcga ggtggttggt
     4201 cggctcgtcg agcatcaaga ggtccggctc ggagagcagc gcctttgcca gcgcgacacg
     4261 ggtcttctgg ccgccggaga gttgcttgac cggatcgctg aacatctcct cggggaagcc
     4321 gaggccgtgc aggaccgcct cggtccggtg ctccatctcg tagccgccat gtgtctcgaa
     4381 ctcggtcgag aggcgcgcgt attcctcaaa catcgcgtcg agcgcgtcgc cgtcgacgcc
     4441 ggccatctgc tgctcgagcg tcgccatccg ctcgccgatc gcgcggacat gcgcgaacgc
     4501 ctcgatcgtc tcctcgtaga gcgtcctgtc gtcggtgaag gtcgcttcct gcgcctggta
     4561 ggcgacgcgc aggccactct ggtagacgat gccgccctcg gtcggctcct cgacaccggc
     4621 gatgatcttc agcagcgtgc tcttgccggc cccgttcgtg ccgaccagcg cgacatgctc
     4681 gcgctcgttc acctgaaagg tgacaccgga gaagatcaca tcggtgatga agcgcttgct
     4741 gacatcctgg acggtcagaa ttgtagtcgc catcgttgtc gctgctcctg ttcgactggc
     4801 ccgacgtcgt cgtcggccac tggtgatgct cgttcgtctg cgattggggg cgttgttctg
     4861 ccgcgtctgg ctgggacggc gggtgacttt gagtcacctc ttgtcaaact agaacatgtg
     4921 ttctaatctg tgcgtggacg ggcggcaggc cccatgagac gggagcatct gaggagtagc
     4981 tcgatgggtg gaacgcaggc acgtggaaac gccggaggca tggggcgctc cgactcggaa
     5041 ggtgctggag ttgtcgtcgc ctgtgtcttc attccgcact ttccactccg ggtagagatt
     5101 ctacgacatc cggagctgga tggcctgccg ctggcgctga ccgacctggc cggcgcgggg
     5161 cgacgacgga tcgtcgagtg ctcgccggag gcgaccgagc gcggtctgcg ggtggggatg
     5221 ccgctgcggg aagcggtcgg tacctgcccg caggccgtca tcctgacctc cgacccggtc
     5281 gcctacgcca acgccttcgc cgatctgctg cgggcgctcg gggaagtcag tcccgctgtt
     5341 gaagcagccg aagcgggcgt tgccttcgtc gacctgcgtg gtctcgaccg tctctacggt
     5401 gggtccggga acatcacggc tgcgctgctg cgggccgtgc caccggccct gcggccacgg
     5461 gttgggctgg ctgccaacaa gttcacggcc cacgtcgccg cgacccaggc gtcaccgggt
     5521 ggcgtccgga tgatcccggc cgcctatgcc aaggcgtttc tggccggcag cccggtcgat
     5581 gtcctgccgg tgccgcttga gatgcggcga cggctggagc gcttcggcct ctaccacctg
     5641 cgcgacattg cccggctgcc gctcggcaag ctccaggccc agttcggccc cgacggccgg
     5701 cgtgtctggg agctggccca cggccaggac agcgctccga tccgtccgct ggcccagctg
     5761 gagcgcttcg tcgagcggtt gccgttaccg tcaccgaccg tccagatcga gacggtcctg
     5821 atggcgctgc gtcaggcctg cgagcgagct tatacccgaa aggaggtgcg tggacgcggc
     5881 gtgcgacagg tgcggctgca actgctgctg gaggaacacc gctcctggga gcggacggtg
     5941 acactgaaag gaacgatcga tgatccatgg acgctcaaca cgacattgcg ctaccggttg
     6001 cagggtctgc aactggaagg ggccgtcgag gaggtcgtcc tggaactgat cgggctgacc
     6061 gacatccacg cccgccagac gcagctcttc gacgagctgg acctgcgccg tcgccagcgc
     6121 agggagatcg gcgaagccgt tcgccagctc aagcagcgct acggtggctc cccgctctac
     6181 cacgttgtcg gcatcgagcc ctggtcgcgc attcccgaac gccgcctcgg cctgttcgcc
     6241 tgggaagcgt gagtggtcat tacatgttga cggtatgctg tcatcatgcg tcgcatcgag
     6301 cagacaggtt gaggaacgat cacgatgaag acagccaccg ttgagctacc cgaagatatt
     6361 ctggcgcttc tggcacagac acggctgggc gatcggacgc agtccgacca ggtcaggatc
     6421 gccgtcgcga tccacctctt tcaggaagga attatctcga tcggcaaggc agccgagttg
     6481 gctggggaac caaggatcgg attcgaacgc ttcctggccg gaatcggaat cgcgccagtc
     6541 cagtacgacg aggagatgta tgcccaagat ctgcgcggga tcgcttaatt gtcacctagc
     6601 aacttcagga actgagcgac ggtcacgacc gcgatattgt gctcgcgcag gtgtgcaatc
     6661 aagttgaggt ctcgcttgat gtcgtcgtcg cgggaaacga gatagtcagc ctcgccgaga
     6721 atagcggttt ccaggacgat gtcgtccttc ggatcgcgac agagttgtag attgcctttc
     6781 ggagcgacga ggacggcttc acgctgtaat cgccttaacc agagcgtcat ccaatcatca
     6841 cgaaagcgaa ccgcgcgacg gacacgcggc cgtgagagga catccgagat ctcatccagc
     6901 aagggttgag agacgaccaa ctggtagtgt ccctctgtgt acagatcaag tacgtgagcc
     6961 ggtgggccaa ctgggttgat aaacgctgaa atccagatgt tggtatcgag cggaacacgc
     7021 caacgcgcat caaccatgcg cagtcagatg ttccgataga cgcacctggc gtgcttcgtt
     7081 gctggccgca gtgatgagat cttcgatctc gtcaggcgtt agattagttt cgacactctc
     7141 tcgaatctct tgcagaagct gcttcatctc cacccgccat tcttcgcgct cttcctttgt
     7201 cattggcagt ggcatgcgaa tgcgcgacgg atcggggtag ccctgggctt tggcgtagtc
     7261 gttgagcgcc tcgcggacga tgtcggtgaa cgagcggccc tgctggacgg ccagatagcg
     7321 gatgagccgg tcgaggttct cgtcgaggta gatgttggtg cgcttcatgg gtgcctcctg
     7381 tcaagcgggt tggtgacatc ataacatcat gatgtcagag aggggtgaca tgttctggaa
     7441 gtcgcctgaa ccggtcaatg aggctgagac gatccggttg ctgaatcagc cccggccgct
     7501 gatcgttgag gcggacgatc ggggcgttcc acgccgagtc ggtgccggtg agcgccagcg
     7561 gcgggtcgtc aggatcgtcg atgcctggcg gatcgacgac gagtggtggc ggacgacgat
     7621 cagccggcgc tacctgctgg tcatgcttga ggacggggtg atacgcacga tctttcagga
     7681 tctgatcggc aatggctggt atgagcagac gtactgagtt gcgagtgccg agtgccgaga
     7741 gtcgagtcgc cgccgcatat gacctgcgcg cggatagctg gctggctcta ctcagcactc
     7801 agcactcagc actcagcact ctctgacgtt ccatgtacgc cgaactgcat ctccatactg
     7861 ccttctcgtt ccacgatggc gcgtcgatgc cggccgagtt ggtcggccgc gccgtcgagc
     7921 tgggatatga ggcgctggcg atcaccgacc acaacgggct gcatggggcg atggagtttg
     7981 cccaggcggc gaagactgcc ggtatccagg cgatcatcgg cgcggagctg acgctcctcg
     8041 acggcagcca cctgacgttg ctggccgaga cggccgcggg ctatgccaac ctctcgcagg
     8101 tgatcacacg ggcgcacctc gacagcgagc ggctcgaccc gcgcctggcg ttggagacgc
     8161 tccgcgagca cacgcgcggc ctgatcctgc tgaccggttg ccgcaacggc gagctggcgc
     8221 tgctggtcga cgcgggccgg atggacgcgg cccggagcgt gctggaccgc taccgtgact
     8281 ggttcgggcc ggagaacgtc ttcgtcgagc tccagcagaa catggtctac ggtgacacgc
     8341 gccggctggc gacgctggcc cggctggcgg aggaggcggg gctggggatc gtcgcgaccg
     8401 gcaacgtcca ctaccacgtt cgcgagcggc accggctcca ggatgtcctc gtcgcgatcc
     8461 gccaccgact gacattggac gcctcgcatc aggtgcggcg cgccaactcc gagttcttcc
     8521 tgcacccgca aagtgagatg gagcgccggt tccgtcacta ccctgaggcg atccggaata
     8581 cgctcgtcat cgccaatcgc tgtgccggct tcgacatgac gcgagatctg cgctatcgct
     8641 tcccggacta cgagcggagc ctggatcatt cgccggcagc gacagcctca ccacccatgt
     8701 cccctgacga ccagctggcg agcgactgct atgcgctggc gcgggagcgc tacagcgccg
     8761 acgcggagga atacgctcgg gcgctcacgc ggctggagca ggagctgggg ctggtcaagc
     8821 gcaacaacct ggcctggttc tttctggtct accgcgacat catgatcctg gcgaagcaga
     8881 tcgctgcgga ggtacgtggg acgggcgcgc gtggagccta cgcgctcccg ccggggcgtg
     8941 gacgcggctc gtctgtctcc tcgatcatct gctacctgat cgggctgtca cacatcgacc
     9001 cgctgcgaca caacctgtcg gtcgggcgct tcctgaatga gagcctggcc tccgcgccgg
     9061 atatcgacat cgatttcccg cgtgacattc gcgagaagct gatcgcccgc gtctacgagg
     9121 tctatggtaa cgaccacgcc gccctggtct gcgcgttccc gacctaccat atccgcagcg
     9181 ccatccgcga catcggcaag acacttggcc tgccggcgcc cgacctcgat cgactggcca
     9241 agctcagcga gcgaacctcg tctgaccggc tcgcggccga gatggaacgg atgccggagt
     9301 ttcgcgaccg actcgacgcg ccgctctggc gcgagctgat cgagctggcg ggccagatct
     9361 ccaggttccc acggcatgtc agccagcatt cgggcgggat ggtaatctcc gcgcaaccgc
     9421 tgacggagct cgtgccggtc cagccggccg cgatggaggg gcgctacatc tgccagtggg
     9481 acaaggattc ctgcgacgac gcgagcttcg tcaagatcga cttcctggcg ttggggatgc
     9541 tctcg
//
LOCUS       DASUGJ010000127         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_433697, whole genome shotgun sequence.
ACCESSION   DASUGJ010000127 DASUGJ010000000
VERSION     DASUGJ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433697"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFV93_02865"
     CDS             complement(<1..430)
                     /locus_tag="VFV93_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5164313.1"
                     /translation="MSDSSEIVVRRFVGGPLLTDCYAIIVPNQGAVMIDAPRDAWQAA
                     IEAAEAQNARMLLAIATHGHWDHITDMARVREQGIPVAANLADANLFADPHGQGLTLP
                     FAVDPVTIDRPLNEGDRLAIGNYELHVLHTPGHTQGSVTIW"
     gene            748..1722
                     /locus_tag="VFV93_02870"
     CDS             748..1722
                     /locus_tag="VFV93_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5164314.1"
                     /translation="MSLVMDEMTRTERVMAAVMGDEVDRIPVCFWHHFKPNGSGRRMA
                     EATINFFEADFDLDIIKIMPDLPYPFPRLSLRDVNDWRLVEPIDIERSRFFRQRAVAI
                     QALRDAVGYETPIVMTVFSPLCEALHFTETREMFFEHARQNPAAVHEALATIAENLRA
                     HIQDCIDAGADGVFFALQGCTDAIMTRDLYRELGRPYDLIALQGARDSWLNILHVHGD
                     RDLHFDEVVDYPVQVMNWSDRLAGPSLREARNKTSKCLMGGWHEFGALSNGPVEEIRS
                     EAEDAVRQTGGRKFILANGCSVPDDTDDQWLHAARQIADELEIPAGQG"
     gene            1858..2400
                     /locus_tag="VFV93_02875"
     CDS             1858..2400
                     /locus_tag="VFV93_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164315.1"
                     /translation="MEISAISKWGGMDRLVEQQHSDAGKSILLLVPDVFFAVNVRNAV
                     RRVGYDPHVLKTTDDLIVAAGEEQPALAVVDLAAVDGDAEWADIGDLARAGLPVLVFG
                     SHKDVDGLRAAKSAGVTRVVSNGQFHREMDDLIQRYALASSCAIADEDLDIDEPLGSL
                     PPGMAEHLPDSEPVRSAIDE"
     gene            2452..>3028
                     /locus_tag="VFV93_02880"
     CDS             2452..>3028
                     /locus_tag="VFV93_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX5164316.1"
                     /translation="MRVFISVDIEGIAGVAGREEGSQGNPEYERARRLMTAEACAAVR
                     GIFNADPDAEVTVADAHGTFRNIIPELLDSRARLVRGKPRPLAMIEGVQNGVDAAVFV
                     GYHGRAGTGDSVLSHTFTGTLADVRVNGTSFGEIGLNAAVAGVYGVPLVLVTGDASVD
                     AEVEALLPGTRTAVVKRGIGALAADGLHPERA"
BASE COUNT          523 a          921 c         1018 g          566 t
ORIGIN      
        1 cccagatcgt gacgcttccc tgagtgtgcc ccggcgtgtg caggacgtgc agctcgtaat
       61 tgccgattgc cagccgatca ccctcgttca ggggccggtc gatcgtgacg ggatcaaccg
      121 cgaacggcag cgtcaacccc tgaccgtgcg ggtcggcgaa cagattggcg tcggccagat
      181 ttgccgcgac cgggatgccc tgctcgcgaa cgcgcgccat atcggtgatg tggtcccagt
      241 ggccgtgggt cgcgatcgcc agcagcatcc gcgcgttctg ggcctcggcg gcctcgatgg
      301 cggcctgcca ggcgtcgcgg ggcgcgtcga tcatgacggc gccctggttg ggaacgatga
      361 tcgcgtaaca atcggtcagc agcggcccac cgacgaagcg ccggactact atctcgctgg
      421 aatcactcac ctcgtcgcct cctccactgc tgcacccggt tcggattgta gcgggtcagg
      481 acagacgagg accgccccgg tgttggggta ccggggcggt cgggtgggga agggggtgtg
      541 gggtgtgtcg cggtgtgggg aaccgctgct atatccgaca atccagacgc gtcttgatcg
      601 ttacgcgtca tcctgcgaga ctcaatgctg gactgtcggc aggagatatc catccgtcgc
      661 cgccagtcgg agggccagtg tggtacgttc cgtctcgaca gggcgttggc gtgtggatgc
      721 gatctgggaa gggaagtagg cgagacgatg agcctggtga tggatgagat gaccaggacc
      781 gagcgcgtca tggctgccgt catgggcgac gaggtcgatc ggatcccggt ttgcttctgg
      841 caccatttca agccgaacgg ctcgggccgc aggatggcgg aggcgacgat caacttcttc
      901 gaggccgact tcgacctcga tatcatcaag atcatgccag acctgccgta tccgttcccg
      961 cgcctgtcgc tgcgcgatgt gaacgactgg cggctggtcg agccgatcga catcgagcgc
     1021 tcgcgcttct ttcgccagcg tgcggtcgcg attcaggcgt tgcgcgacgc agtcggctac
     1081 gagacgccaa tcgtcatgac ggtcttctcg ccgctgtgtg aggcgctgca cttcaccgaa
     1141 acgcgtgaga tgttcttcga gcacgcgcgc cagaatccgg cggctgtcca cgaggcgctg
     1201 gcgacgattg ccgagaacct gcgcgcccac atccaggatt gcatcgacgc cggcgctgat
     1261 ggcgtcttct tcgcgctgca gggctgtact gatgcgatca tgacccgcga cctgtaccgt
     1321 gagctgggac ggccctacga cctgatcgcg ctgcaagggg cgcgggacag ctggctgaac
     1381 atcctccacg ttcacggcga ccgtgatctg cactttgacg aggttgtcga ctatcccgtc
     1441 caggtcatga actggtccga ccggttggcc ggaccaagcc tgcgcgaggc gcgaaacaag
     1501 acgagcaagt gtctgatggg tggctggcac gagttcggcg cgctgtcgaa tggaccagtg
     1561 gaagagatcc gctccgaagc cgaagatgcc gtccggcaaa ccggagggcg caagttcatc
     1621 ctcgccaacg gctgctccgt cccggacgat acggacgacc agtggctgca cgccgcccgc
     1681 cagatcgccg acgagctgga gattccggca ggccagggat gatccccgcc ggtcgctgac
     1741 gcgctgacgt tgagtcaaga tgtacataca gtcttgaaac aggctcgcga ttcccctact
     1801 ctagaagggg atcgcgagcc cgttcataca agtacattga attccggcgg gttgttgatg
     1861 gagataagcg cgatctcgaa gtggggcggg atggacagat tggttgagca acagcacagc
     1921 gatgcgggta aatccattct gttgctggtc ccggacgtgt tcttcgcggt caatgtccgt
     1981 aacgctgtcc ggcgtgtcgg ttacgatccg catgtgctca agacgaccga cgacctgatc
     2041 gtcgcggccg gtgaagagca accggcgctg gcggttgtcg acctggcggc cgtcgacggg
     2101 gatgctgagt gggccgacat tggtgatctc gcgagggctg gtctgccggt gctcgtgttc
     2161 gggtcgcaca aggatgtcga cgggttacgc gccgccaagt cggccggcgt cacgcgcgtc
     2221 gtctccaacg gccagttcca ccgcgagatg gacgacctga ttcagcgcta cgcgttggcg
     2281 tcgtcgtgtg cgattgctga cgaagacctt gacatcgacg aaccactggg tagcctgccg
     2341 cctggcatgg ctgaacacct gcccgatagc gagccggtgc ggtccgccat cgacgaatag
     2401 cacgtcatcg attgctgaga gcttgccgtt tcaacggacg gagaataccg cgtgcgtgtc
     2461 tttatttcgg tcgatatcga agggatcgcc ggggtcgccg gtcgcgagga aggcagccag
     2521 ggcaatcctg agtacgagcg agcccgccgg ctgatgaccg ccgaagcctg cgcggctgtc
     2581 cgcgggatct tcaacgccga tcctgacgct gaagtcaccg ttgcggacgc gcacggcacc
     2641 ttccgcaaca tcattcctga actactcgat tcccgtgcgc gactcgtgcg tgggaagccg
     2701 cgaccgctgg cgatgatcga gggcgtccag aacggcgttg acgccgctgt cttcgtcggc
     2761 tatcacggcc gagccggaac cggagattcc gtcctcagcc ataccttcac cggcacgctg
     2821 gccgatgtac gcgtcaacgg gacgtcgttt ggcgagatcg gtctaaacgc cgccgttgca
     2881 ggcgtctacg gcgtcccgct cgtgctcgtt accggtgacg cgtcggttga cgctgaagtc
     2941 gaggcgctgc tgcccggtac gcggaccgcc gtcgtcaagc gtggcatcgg cgcgctggct
     3001 gccgacgggc tgcacccgga gcgcgcct
//
LOCUS       DASUGJ010000128         6029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1823473, whole genome shotgun sequence.
ACCESSION   DASUGJ010000128 DASUGJ010000000
VERSION     DASUGJ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823473"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            700..2043
                     /locus_tag="VFV93_02885"
     CDS             700..2043
                     /locus_tag="VFV93_02885"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX5164317.1"
                     /translation="MLLRQGYGLVAKLYALGATILALALLGASLLTSWPVIDRDILIT
                     AAVLGVISLLLEFIDFPLLIGGATSFSTVAYIAMVFMLPFPLPAIVGAAVVLAADLRT
                     RRPMFAMVFNAANFVLTFGVSSLIWHLTYGSKSIAELPHSPVALLVVLLIIIVFYSIN
                     VFLMNGLIAIVGQRSIKYIWLTNDFDMFLPYICLEVIGTLVALIWTSTPILTPLLIIP
                     AITSYIAFEMIHRLQHQTQEAMIAMADAIDQRDPYTADHSRRVAELAVKIAEVHGVNE
                     RDIERLRIAARMHDIGKIGIGNDLLHKPGKLTDDEWEILRAHPVIGEQLLKPYRQFRH
                     ETRLVRHHHERWDGKGYPDRVRGQAIPLGARIIAVADTFDAMTTSRPYRPALSRQYAI
                     DEIRNGALSQFDPQIVASFLQVMEESSKIRSITGEDLPPSVKELEHAPAWYSSLP"
     gene            2195..2830
                     /locus_tag="VFV93_02890"
     CDS             2195..2830
                     /locus_tag="VFV93_02890"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HEX5164318.1"
                     /translation="MLILILAVVVSLSIGVLTGGKVSNLAQTRFRYLPLLIVALMVQI
                     LIFSELFGREPIIHRIGPYIHIATLATSFGVMVMNRHIPGMKIIMIGAGLNLLVITAN
                     GGFMPISESALRIAGQEEEMVHHTPRHDSEDYLLPNSRLSAGKANLLFLGDAIPFREG
                     WPIATVVSIGDLILAAGAGTAVILGMRRRDSEDEEDESTLVLHESNHTPAH"
     gene            2903..3490
                     /locus_tag="VFV93_02895"
     CDS             2903..3490
                     /locus_tag="VFV93_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5164319.1"
                     /translation="MSEDGQTQTAAAGCLPGEPADDALVLAARHDRQAFSPLYERYAT
                     RVYRYARARTGSETAAEDILSETMLGALEALDRYNPRRGSFASWLFTIATRRIIDRQR
                     RDSRFRTLLERAWEPAGTQEDTLTTLVRSDEAARLRAKLAQLPDRDRELILLRYSAEL
                     TSAEIGQVAGMSAGAVRIRLMRLLDQLANDLGDVR"
     gene            3487..5766
                     /locus_tag="VFV93_02900"
     CDS             3487..5766
                     /locus_tag="VFV93_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164320.1"
                     /translation="MSTEHDSLTVDPRLETLLRGILASDDLQPTQNERVRARLGLLAT
                     EPAGAATLTIPASRAQIATPEPEAAPLAAPPQTRLRRQWLQFALAAVAFIAVGVLLVL
                     LFGSGNDDSSDSRTGTEASPITGMLDDALTVEEARARLSFDLIVPDAFPDALSIAGVF
                     VAENNRSATIWLANDGETTPRITVTEILGQVTTPSAENMQRALLESLDQPPATSLPQP
                     SQTTIIVAGRTILQTVIYGGNGESLYLYSWSQGEIHLDVDSFGGAKEDIEQVVNAFAS
                     IPDIDATPEPATPSADDEDVSTLPTPDEMGMYRDITLDQAQAIVPFEIVVPEQVPDGL
                     DQAFITVIEAPQVSGEPNYRVEMSFGLAGDESPSQSIQFLQRDSPPPMTGIPEGATPL
                     GTLNGIDVFQTNETNAAGDPLITYQWQHNDVGYLIIARTVGDLTPELVDKLLQAIFVS
                     FTPTAEGETARLEQERAEMMQFPIPDTCAASDWAGPDFRVGQQYPTAYYLDGDGLSLW
                     TTHGLLFAGENEITWLADAPLTTTEALSGPETIQAVQAGADGATTEIPIDVTEQIYNV
                     RDTERAWWSTVNFPSEGCWEIEVSLGVHTLSATVYVYPQPPSGEFPQIDGAVMVDAAQ
                     LGSDDLPFAASDVIGTIHVVATNPIDVNSYYSVPTQALRDAGWSGGGFLTQQYASYSG
                     WANGNRIVLFIIIPGGAQDVFRDEVGAIDLGPFPDALDEVGDNEVLIYTRTAQCEEAT
                     PDDCMRKAFEAVNGPSGRP"
BASE COUNT         1152 a         1988 c         1777 g         1112 t
ORIGIN      
        1 tcaggcagag tcgccggtac ttgtttccta gtacttcggc acccgattcg cggtacagat
       61 gtattgcgta tgtcggtact atctgctatc gtatccacgt accgcggaaa cacagacggg
      121 ttcggatcaa aacagggttc acggaccgaa aggggaagag gggacaacat gaagcgcctc
      181 aagggacaga tcgtgtacag cgcgctgtgg ctcaccggca tcatctcact ggtggttgcc
      241 tcgggtgcgc cgcacaagtt caccggttgg ccgtaggtcg gacgaccgat tactggcgga
      301 tttagtactc cggctcgccg tctgacgtga gccgccattg ttgagaaagg tacgagcatg
      361 aagcgcatta agggcaatat cgtttattcc gctctctggc tgaccggcat tatctcgctg
      421 gttatcgcct ccggcgcgcc gcacaagttc actggctggc cgtaggctcc gtttccacgc
      481 cagacaacaa cgacaattca aacaccccgg ccacgtgact tgggtcacga ccacatctag
      541 tactctaggt gggttggccg gggtgtttct tgcgcgcgag aatgcgtacg attcccatcg
      601 gcatggtgca atcgctgtcg tgaacgttgc gtagcggttg tccgtccagg gattgcgtcg
      661 caccgcagcg catgattgag gctgtggatc cggaggtcgt tgttgttgcg ccaggggtac
      721 gggctcgtcg cgaaactcta cgcccttggg gcgacgatcc tcgcgctcgc gctgcttggc
      781 gcctccctgc tgaccagttg gcccgttatc gatcgcgaca ttctcatcac ggctgcggtg
      841 ctcggcgtca tcagcctcct gcttgaattc atcgacttcc cgctgctgat tggcggcgcc
      901 accagcttct cgaccgtcgc ctatatcgcg atggtcttca tgttgccgtt cccgctgccg
      961 gccatcgtcg gcgcggccgt cgtgctggcg gcggaccttc gcaccagacg accgatgttc
     1021 gcgatggtgt tcaacgcggc gaacttcgtc ctgaccttcg gcgtcagtag tctgatctgg
     1081 cacctgacct acggctcgaa atcgattgcc gaattgcccc acagtccggt ggcgctgctc
     1141 gtcgtgctgc tgatcatcat cgtcttctac tcgatcaatg tcttcctcat gaacgggcta
     1201 atcgccatcg tcggccagcg ctcgatcaag tacatctggc tgaccaacga tttcgacatg
     1261 tttctcccgt atatctgcct tgaggtgatc ggaaccctcg tcgccctgat ctggacgtcg
     1321 acaccgatcc tgacgccgct gctcatcatt ccggccatca cctcgtacat cgccttcgag
     1381 atgatccacc gtctccagca ccagacccag gaagcgatga tcgcgatggc cgatgccatc
     1441 gaccagcgcg atccgtacac ggccgaccac tcgcgccggg tggccgagct ggccgtcaag
     1501 atcgccgagg tccacggcgt caacgagcgc gatatcgagc ggctgcggat cgcggcccgc
     1561 atgcacgaca tcggcaagat cggcatcggc aacgatctgc tgcacaagcc cggcaagctg
     1621 accgacgacg agtgggagat cctgcgcgcc cacccggtca tcggcgagca gttgctcaag
     1681 ccctaccgtc agttccgcca cgagacacga ctggttcgcc accatcacga gcgctgggac
     1741 ggcaagggct acccggaccg ggtgcgtggc caggcgatcc cgcttggggc gcgcatcatc
     1801 gccgtcgccg atacgttcga cgcgatgacg acctcgcgac cgtaccggcc ggccctctcg
     1861 cgccagtacg ccatcgacga gattcgcaac ggggcattga gccagttcga cccgcaaatc
     1921 gtcgccagtt tcctccaggt catggaagaa tcgagcaaga tacgctcgat caccggcgag
     1981 gacctcccgc cgagtgtcaa agaactggaa cacgcgccgg cgtggtactc ctcgctgccg
     2041 tagccaccca cccgagcgcc cgtgacgtcc agccaaccgg gcagcgcttg atgtggttgt
     2101 gacgctcttg tgataatgac gccatctgcc gacccacggc gttggatacg atggacgtcg
     2161 tgcatcgccg catcggttcc ggaggagtgg tctcgtgctg attttgatcc ttgccgtcgt
     2221 cgtgagcctg agcatcggag tcctgaccgg cggcaaagtc tctaacctgg ctcagacgag
     2281 gttccgctat ctaccgctgc tcatcgtggc gctgatggtg cagattctga tcttctcgga
     2341 gctgttcggc cgcgagccga tcatccaccg catcggtcca tacattcata tcgcgaccct
     2401 cgccacgtcg ttcggggtga tggtgatgaa ccggcatatc ccgggtatga agatcatcat
     2461 gattggcgcc gggttgaacc tgctcgtcat cacggccaat ggcggcttca tgccgatctc
     2521 cgagtcggcg ttgcgcatcg ccggccagga agaggagatg gtgcatcaca cgccacgaca
     2581 cgatagcgag gactacctcc tccccaacag ccgtctcagc gcaggcaagg ccaacctgct
     2641 cttcctcggc gacgccatcc cgtttcggga agggtggccg atcgcgaccg tcgtctcgat
     2701 cggcgacctg atcctggcgg ccggcgcagg caccgctgtc attctcggca tgcgccggcg
     2761 tgacagcgaa gatgaggagg acgagtcgac gttggtcctg cacgagagca accatacccc
     2821 cgcacactag ccagcgaacc tgctccgcag gtcatctgta acagcgacgc tcctcgcgcg
     2881 aaagcagagt gagggggttc gcatgagcga ggatggccaa acccagactg ccgcagccgg
     2941 ttgcctgccg ggcgagcctg ccgacgacgc gctggtgctg gccgcccgcc acgaccggca
     3001 ggcgttcagc ccgctctacg agcgctacgc cacccgcgtc taccgctacg cacgggcgcg
     3061 aaccggctcg gagacggccg ccgaggacat cctcagcgag acgatgctcg gcgcgctcga
     3121 agcactcgac cgctacaacc cccgccgcgg cagcttcgcc agctggctct tcacgatcgc
     3181 aacccgccgc atcatcgacc gccagcgccg cgacagccgc ttccggacgc tcctcgaacg
     3241 ggcctgggag ccggccggca cccaggaaga cacgctgacg accctcgtcc gcagcgacga
     3301 agcggcccgg ctccgcgcga agctggcgca acttcccgat cgcgaccgcg agttgatcct
     3361 gctgcgctac agcgccgaac tgaccagcgc ggagatcggc caggtcgccg gcatgtcggc
     3421 cggggccgtc cgcatccgcc tgatgcgcct gctcgatcaa ctggccaacg accttggaga
     3481 cgtgcgatga gcaccgagca cgacagcctg actgtcgatc cccgcctgga gacgctgctg
     3541 cgcggtatcc tcgccagcga cgacctgcag cctacccaga acgaacgcgt ccgcgcccgg
     3601 ctcggcctgc tggcaaccga acctgccggt gcggcgacgc tgacaatccc ggcctcccgc
     3661 gctcagatcg ccaccccgga accggaagcc gccccgctgg ccgcgccacc ccagacgaga
     3721 cttcgccgcc aatggctcca gttcgcgctg gccgccgtcg ccttcatcgc cgtcggggtg
     3781 ctgctggtgt tgctgttcgg cagtgggaat gatgactcgt ccgactcgcg caccggaacc
     3841 gaagcatcac cgatcaccgg gatgctcgac gacgcactaa cggttgagga agcgcgggcg
     3901 cgattgtcgt ttgacctcat cgttcccgat gcatttcctg acgcactctc gattgcaggg
     3961 gtgttcgttg ccgagaataa ccgctcagcg acgatctggc tcgcgaacga cggagagaca
     4021 acgcccagaa tcacggtgac agaaatcttg ggacaggtga caaccccgtc tgccgaaaat
     4081 atgcaacgcg ctctgctgga gtcactggat caaccgccag ccacgtcgct cccccagcca
     4141 agccagacga ctatcatcgt tgccggccgc accattctcc agactgtcat ctacggcggg
     4201 aacggtgagt ccctgtatct ctattcatgg agccaaggag aaatccatct cgatgtcgat
     4261 agcttcggcg gcgcaaagga agatatcgaa caggtcgtga atgcatttgc gtcgataccc
     4321 gatatcgatg ccactcctga accggccact ccctcagccg acgatgaaga cgtttcgacc
     4381 ctgccgacac cagacgagat gggaatgtat cgcgatatca ccctcgacca ggcgcaggca
     4441 atcgtcccgt tcgagatcgt cgtcccagaa caggttccgg atggccttga tcaggccttc
     4501 atcacggtca ttgaagcgcc acaggtatcg ggcgagccga actaccgagt ggagatgtcg
     4561 ttcggcctcg caggcgatga gtcaccgtcg caatcaatcc agtttctaca acgcgactct
     4621 ccaccgccga tgaccgggat acccgagggc gcaacgcctc tcgggacgct caacgggatc
     4681 gacgtcttcc agacgaacga gacgaacgca gccggtgacc cattgatcac gtaccaatgg
     4741 cagcataacg atgttggcta tctgatcatc gcccgaacgg ttggcgacct cacccccgaa
     4801 cttgtcgaca agctgctgca agccatcttc gtctcgttca ccccgacggc tgaaggagag
     4861 accgcacggc tagagcagga gcgggcagag atgatgcagt tcccgattcc cgacacctgc
     4921 gcggcgagcg actgggccgg gccggacttc cgggttggcc agcaataccc aaccgcctac
     4981 tacctcgacg gcgacggcct gtcgctctgg acgacccacg gcctgctctt cgcaggtgag
     5041 aacgagatca cctggctcgc cgatgcgccg ctgacaacga ctgaggcgtt gtccggccca
     5101 gagacgatcc aggccgtgca ggccggcgct gatggagcga cgaccgagat tccgatcgat
     5161 gtcaccgagc agatttacaa cgtccgcgat accgaacgcg cctggtggtc aaccgtcaac
     5221 ttcccgtccg aaggctgctg ggagatcgag gtgtcgcttg gcgtccacac actctcagcg
     5281 accgtctacg tctacccgca gccaccctcc ggcgagttcc cccagatcga cggcgcggtc
     5341 atggtcgatg cggcgcaact cggcagcgac gatctcccct tcgccgccag cgacgtcatc
     5401 gggacaatcc atgtcgtcgc gaccaacccg atcgatgtca actcgtatta cagcgtcccg
     5461 acccaggcgt tgcgcgatgc gggctggtcg ggaggcggat tcctgacgca acagtatgcg
     5521 tcctacagcg gctgggccaa cggcaaccgc atcgtcctgt tcatcatcat tcccggtggt
     5581 gcacaagacg tgttccgtga cgaggtcgga gccatcgatc taggcccgtt ccccgatgcg
     5641 ctcgatgagg ttggcgacaa tgaggtgctg atctacacac ggactgcaca atgcgaggag
     5701 gcaacgccgg acgactgcat gcggaaggca ttcgaagcgg tgaacgggcc ttccggtcgg
     5761 ccgtgagcca agaagggatc tatccatgtg gatctggatt gccgctggac ttgtcgtgct
     5821 tggcctaacc acgctgcaga tctatctctc gatccgctgg ggccgtggcg ctcgtggagg
     5881 atcgttccag gaaccattga acgagatcca gaacaccacg ccctggaagg gaccgccgat
     5941 gttctgagtg taattaacca tccgttgatc cgtgcaatgc gccgaacccc gctatatgtc
     6001 acgttcctca gatggcggac atgtgaaga
//
LOCUS       DASUGJ010000129         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_433794, whole genome shotgun sequence.
ACCESSION   DASUGJ010000129 DASUGJ010000000
VERSION     DASUGJ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433794"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..>2355)
                     /locus_tag="VFV93_02905"
     CDS             complement(71..>2355)
                     /locus_tag="VFV93_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164321.1"
                     /translation="PQQAFPYAALVSENQRRSRSDPEYELIDTGVFAEDRYFDTYVEY
                     AKRTPNDILIRLTVVNRGPDPAPLHVLPTLWFRNTWSWGRDDRRPVLMAAAANDHALV
                     HARHPRIGEYWLAAQGTPDLLFTENESNAERLWGTANQSPYVKDGINDYVVHGLRTIN
                     PERVGTKVAVQYHVELAPKASWTQLLRLSRERHPTPFEGGETLIGTRRSEADRFYEPL
                     IQAGAPDDLRRIQRQAFAGLLWSKQHYHFDVEQWLDGDPGQPPPAAARKRGRNRDWRH
                     LNNDEIISMPDTWEYPWYAAWDLSFHCIPLALIDPEFAKQQLILLLREWYMHPNGQLP
                     AYEWDFGDVNPPVHAWAAWRVYTIERRPTGRGDLEFLERVFHKLLLNFTWWVNRKDMG
                     GRNVFQGGFLGLDNIGVFDRNATLLDGGHLDQSDGTAWMGMYCVSMLVIALELAQQKP
                     AYEDVATKFFEHFLYIAAALNNLGGQGIALWDEQDGFFYDCLHLPDDRVFPLKVRSMV
                     GLIPLLAVETIEASRLATLPGFKRRMRWFLVHRPDLASLVARWDAPHVGERQLLALVP
                     GHRLKRVLARMLDPNEFLSDYGIRSLSRSHADAPYTLLVSGTEHRVDYQPAESTTPLF
                     GGNSNWRGPIWFPINYLLIEALQKFHRYYGDDFVVEHPTGSGTYVTLGDLADDLAQRL
                     IAIFTSDASGRRAVFGDNAIMQYDPHWCHSSPFHEYFHGDTGAGLGASHQTGWTALVA
                     KLLEEFQPARAQRGSQSLRS"
BASE COUNT          440 a          706 c          769 g          440 t
ORIGIN      
        1 cttggcaaat attgtgttta ctttgttgac aatcgcctcg tggcaaccgg caggcggcat
       61 tccggccaac tcacgatctc aacgattgcg atccgcgctg agcacgtgct ggttggaact
      121 cttccaacag cttagccacc aatgccgtcc agcccgtctg atggctggcg ccgagccccg
      181 cgcccgtgtc gccgtggaag tactcatgaa acgggctgga gtggcaccag tgtgggtcgt
      241 actgcatgat ggcgttgtcg ccgaagacgg cacgccgccc gctggcgtcg ctagtgaaga
      301 tggcaatcaa gcgttgcgcg aggtcgtcgg ccaggtcccc gagggtgaca tatgtaccgg
      361 agccggtcgg gtgctcaacg acgaagtcgt cgccgtagta gcgatgaaac ttctggagcg
      421 cctcgatcag cagatagttg atcgggaacc agatcggacc tcgccagttg gagttgccgc
      481 caaagagtgg ggtcgttgac tccgccggct ggtaatcgac tcgatgctca gtcccgctga
      541 cgagcagcgt gtagggagcg tctgcgtgag agcggctaag cgagcggatg ccatagtcgc
      601 tgagaaattc gtttggatcg agcatccgcg ccaacacccg cttcaaccga tggccgggga
      661 cgagtgccag caactgtcgc tcgcccacgt gcggcgcgtc ccagcgggcg acaagggatg
      721 ccaggtccgg ccggtggacg aggaaccagc gcatgcgtcg cttgaagcct ggtaacgtcg
      781 ccaggcgcga cgcctcgatc gtctcgactg ccagcaacgg gatgagcccg accatcgagc
      841 gtaccttcag cgggaagact cggtcgtcgg gaagatgcag gcaatcgtag aaaaacccat
      901 cctgctcgtc ccagagcgcg ataccctggc cacccaggtt gttgagcgcg gcggcgatgt
      961 agagaaagtg ctcgaagaac ttcgtcgcga catcctcata ggccggcttc tgctgggcga
     1021 gttcaagggc gatgacgagc atgctcacac agtacatgcc catccaggcc gtcccgtccg
     1081 attggtcgag gtgcccgccg tccagcagcg tcgcgttgcg atcgaagacg ccgatgttgt
     1141 ccagccccag gaagccgccc tggaagacgt tccgcccgcc catatcctta cgattcaccc
     1201 accaggtaaa gttcagcagc agcttgtgga acacccgttc gaggaactcc agatcgccac
     1261 gcccggtcgg ccgccgctcg atcgtgtaga cccgccaggc ggcccaggca tggacaggcg
     1321 gattgacatc gccgaagtcc cactcgtagg ctggtagctg gccgttgggg tgcatgtacc
     1381 attcgcgtaa cagcaggatc agttgctgct tggcgaactc agggtcgatc agcgccagcg
     1441 ggatgcagtg aaagcttaga tcccaggcgg cgtaccaggg atattcccac gtgtccggca
     1501 tcgagatgat ctcatcgttg ttgaggtgcc gccagtcccg gttgcgcccg cgcttgcggg
     1561 cagccgccgg tgggggctgc ccgggatcgc catcgagcca ctgctcaaca tcgaagtgat
     1621 aatgctgctt tgaccacaac agtccggcga atgcctgacg ctggatccgg cgcaggtcgt
     1681 caggggcgcc ggcttggatg agcggttcat agaagcgatc cgcctcggaa cggcgcgttc
     1741 caatcagcgt ttcgccaccc tcgaacggtg ttgggtggcg ttccctggag agacgcagga
     1801 gctgcgtcca ggatgcctta ggcgcgagtt cgacatgata ctggacggcc accttcgtgc
     1861 cgacacgctc cgggttgatt gtccgtagac cgtggacgac gtagtcgttg atgccgtcct
     1921 tgacgtaggg actctgattg gcggttcccc agagccgctc ggcgttgctc tcgttctcgg
     1981 tgaagagcag gtccggcgtt ccctgagccg ctagccagta ctcgccgatg cgcgggtgcc
     2041 gcgcatggac aagtgcgtga tcgttcgcgg cggccgccat cagaactgga cgccggtcgt
     2101 cacgccccca cgaccatgtg ttgcggaacc agagcgtcgg caggacatgc agcggtgcgg
     2161 ggtcagggcc acggttgacg acggttagac ggatgagaat gtcgttcggc gtgcgtttcg
     2221 cgtactcaac gtacgtgtcg aagtagcgat cttcggcgaa gacgccggtg tcgatcaact
     2281 cgtactcggg atcgctacgc gaacgccgct ggttctcgga gacgagcgcg gcgtacggga
     2341 acgcttgctg cggat
//
LOCUS       DASUGJ010000130         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_433858, whole genome shotgun sequence.
ACCESSION   DASUGJ010000130 DASUGJ010000000
VERSION     DASUGJ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433858"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VFV93_02910"
     CDS             complement(<1..835)
                     /locus_tag="VFV93_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164322.1"
                     /translation="MSSLVTADARPALAVSVASCLVFAMAGLTGSSPLALLALIVAVA
                     LACVSPVGAIAAAVASLPYFYCPLDIGGQHVAASELLLFAALLGTGFRVLVAAAESPS
                     GALRNVQRQLGPVLRSPLAIAAAGLVVLGGLLVLMPYDPTHRAESLREWRWTLLEPAL
                     LLGLLIWHRRDPLLRELVAVALIAGGTVASLHALGDYALGGGVTVAGVTRIAGPYPHP
                     NALALMTVRIAALAFVWAVVDRRVRRWILPLLVICAVTVAATFSRGAMLAGLVAVAIV
                     LV"
     gene            complement(813..2192)
                     /locus_tag="VFV93_02915"
     CDS             complement(813..2192)
                     /locus_tag="VFV93_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164323.1"
                     /translation="MIPSFDPALWRRASWTVLTLAVVAFICLGLAEQGSRGGGLLIPP
                     GSPLPFERQRVFGLNLDALSSVDALNWLNAAGNPNLALMVLPVDADVVQSLALEDQRE
                     NGLAAIETLRAAAGGSPLALCLRRPAETVGGLAIAQAAVESIKARFPDQVIYVRACDP
                     DNVPGWQGNIDEAARPDAEVRASDDTLIPLAGGPLITLDAIDNVRELDPDRLSLNAHD
                     RYSIYSFEASEPLSTGDVERVAGALADTSHTALVLVAPIDGVDPAALISTISPVVLAG
                     GELPEGFSGVNAPAMHINDGWQHSTVGTTQYLRAASSGAALEVEFVGTDISLIAVESP
                     DSGLINVWLDPASQTAPPTTILDLSSTQARDSAIPVATGLPAAHHRLIMQVVTEEGQS
                     VTISGLFVTGKPPTAWTVTMAAAVVLLAAVAALSERCYTAIIAIRRRSGPPQRRSRTG
                     HPRVFARDR"
BASE COUNT          497 a          784 c          848 g          350 t
ORIGIN      
        1 tgaccagcac gatcgcgacg gccacaagcc cggccagcat cgccccgcgt gagaaggtcg
       61 cggcaactgt cacggcgcag atgacgagca acggaagaat ccagcgccgg acacggcgat
      121 cgacgacggc ccagacgaag gcgagagcgg cgatccggac cgtcatcaac gcaagggcat
      181 tgggatgcgg atacggaccg gcgatgcgtg tcacgcccgc aaccgtgacc ccgccgccga
      241 gtgcgtagtc gccgagtgcg tgcagcgacg cgacggtacc accggcgata agggcaaccg
      301 cgacgagttc gcgcaggagg ggatcgcgcc gatgccagat cagtagaccc agcagcaacg
      361 ccggctcgag cagcgtccag cgccattcgc gcaagctctc ggcccggtga gtagggtcat
      421 acggcatcag gacgagcagt ccacccagca cgacaagtcc ggctgccgcg atcgcgagcg
      481 gcgacctgag caccggaccg agttgacgct gcacattgcg aagtgcacct gatggtgact
      541 cagcggctgc gaccaacacc cggaaccccg tcccgagcaa ggcggcgaag agcagcagtt
      601 cagaagccgc gacgtgctgc ccaccaatgt caagcgggca atagaagtaa ggcaggctgg
      661 caacggcggc agcgatcgca ccgaccggtg agacgcaggc cagcgcaacg gcgacgatca
      721 aggccaacag cgccaacggg ctggagccgg tcagcccggc catcgcgaac accaggcaac
      781 ttgcgacgct gacggccagc gccggcctgg cgtcagcggt cacgagcgaa gacacgcgga
      841 tgtccagttc tcgaccggcg ttgtggcggg cctgagcggc ggcgaatcgc aatgattgcc
      901 gtgtagcaac gctcggagag ggcggcaacg gctgccagca gcacgactgc ggcggccatc
      961 gttaccgtcc aggctgtcgg cggcttgccg gtcacaaaca gccctgagat cgtcacgctc
     1021 tgcccctcct cggtaacaac ctgcatgatg agccggtggt gcgcggcagg caggccggtg
     1081 gcgaccggga tggcggaatc acgcgcctgg gtgctcgaga gatcaaggat ggtggtcggc
     1141 ggagccgtct gcgaggccgg atcaagccag acgttgatca ggccgctgtc cggcgactcg
     1201 accgcgatca gcgagatgtc ggtaccgacg aactccactt cgagcgccgc tccggaagaa
     1261 gccgcgcgta gatactgcgt cgtcccgacc gtcgaatgct gccatccgtc attgatatgc
     1321 atggccggag cgttgacgcc ggagaaccct tcgggcaact ccccacctgc gaggacgacg
     1381 ggggagatcg tggaaatcag cgctgctgga tcgactccgt cgatcggcgc gacgaggacc
     1441 agagcggtgt gagacgtatc ggccagcgca ccggccaccc gttcgacatc gccggtcgac
     1501 agcggttcgg aggcctcgaa cgaatagatc gagtagcgat cgtgcgcgtt caggctgagc
     1561 cgatcgggat ccagttcgcg aacgttgtcg atcgcgtcga gtgtgatcag gggtccaccg
     1621 gcgagaggga tcagcgtgtc atcggacgcg cgcacttctg catccggccg ggctgcttcg
     1681 tcgatgttcc cctgccaacc aggcacgttg tccggatcgc aggctcgcac gtatatcacc
     1741 tgatcgggga agcgcgcctt aatcgactcg acggcggcct gggcgatcgc aaggccacca
     1801 accgtctcgg ccgggcgacg caggcagagc gccagtggag atccgcccgc cgccgcccgc
     1861 aatgtttcaa tggcggccag gccgttctcg cgctgatctt cgagcgccag cgactgcacg
     1921 acatcggcat cgaccggcag gaccatcagc gcaagattcg ggttaccggc tgcgttgagc
     1981 cagttcagag catcgacgga cgagagcgcg tcgaggttga ggccgaagac acgctgtcgt
     2041 tcgaacggca gcggcgagcc tggtggtatc agcagtccgc cgcctctgga gccctgctcg
     2101 gcgaggccga gacagatgaa cgcaacgaca gccagtgtca ggacggtcca gctcgcgcgc
     2161 cgccagaggg ccggatcgaa tgacggaatc acgcgttacc tcgcggccag tctggttgcg
     2221 tcggggcgcg gcgaaccgaa acgacttgca tggttcgcga ttgtaccaat ggcaggcatc
     2281 caacgatggc ttgagtacac ttcgccagtg gattggagtg ggcgatgcga cagcggcaac
     2341 cggcgctcgg cattatcggc ggcagcggtc tctacggcct gagttctctc acgaacgttg
     2401 aagaggtcga ggtcgaaacc ccgtggggaa agccgtcgga agcgatccgg atcggtgaga
     2461 tcgccggcca gccggtcgc
//
LOCUS       DASUGJ010000131         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_851185, whole genome shotgun sequence.
ACCESSION   DASUGJ010000131 DASUGJ010000000
VERSION     DASUGJ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851185"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFV93_02920"
     CDS             complement(<1..666)
                     /locus_tag="VFV93_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164324.1"
                     /translation="MSDQRALVRTGQSGILLRTDQANPRVVRGLARLDGQPVDRFNLA
                     DPANRYEASFPDDRAIGHLFALDDDGYWKELGEATGTVHLPVRRFLRFENGHLPLASA
                     DLDLIEPLQLLRDVRLTGKEITDDGLDRLSGLRALRVLALLYTATTDAGLATISHLST
                     LHDLDIRASQLTDNGLARLRDLSELQSLALAHAPIGRRGAVQIGDLRQLRRLSLHDVP
                     LGDD"
     gene            complement(666..1511)
                     /locus_tag="VFV93_02925"
     CDS             complement(666..1511)
                     /locus_tag="VFV93_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164325.1"
                     /translation="MRSRQLMPALDPATVEENQLRARIDSLREQLTAAVVELEQLRST
                     LLAFESRYDARIGVLMVEVDRVELEIAILRRRIAALRESIDAWRAVESSIEREFAAER
                     ERVDAEAREASGARRRARELPPPPEPEVAAAIRSQYRKLARKFHPDVAGEEHRERHED
                     VMRRINVAMEMHDLGTLLDLERELPVGDDDVPGPTRGARIAWATTEIGRLEGQLAKRT
                     GELASLRSTSLHQLWQRIEREPALLDRLESDLRSELTTAQMERHALASQYDDLVKQLS
                     LSGRA"
     gene            complement(1637..2395)
                     /locus_tag="VFV93_02930"
     CDS             complement(1637..2395)
                     /locus_tag="VFV93_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5164326.1"
                     /translation="MPSASIVFVAPHPDDIALSCGGTVAQAARTDSPTIVTLFVGQPT
                     DQVSDFARFQHERWGIDGASVAASRRAEDEAAARALGERVSHSWLDHLDAIYRQAAYA
                     SDDALFGTLHDDDLLLIDVLVAELAALDAAKYFVPLAAGNHVDHQLAFRAGRRLAARG
                     IAVWAYADVPYVIDNQRAVTDRLARGTVREARVTWLDDEAFERKCRAVECYPSQLPVL
                     FRDLGDHRAALDRYARQVGGGQRAEVCWRVLPSR"
     gene            complement(2395..>2578)
                     /locus_tag="VFV93_02935"
     CDS             complement(2395..>2578)
                     /locus_tag="VFV93_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEX5164327.1"
                     /translation="QLAEIQGETLRLIDEHGSLTLAQFRDHFGSSRKYAQAVLEYFDQ
                     QRITRRVGDARVRGSA"
BASE COUNT          397 a          835 c          933 g          413 t
ORIGIN      
        1 gtcgtcgccc agcggaacgt catgcaggct gagacggcgc agttgtcgaa gatcgccgat
       61 ctggaccgct ccgcgccggc cgatcggtgc atgcgccagt gccagcgatt gaagctccga
      121 gagatcacgg aggcgggcga ggccgttgtc ggtcagttgg ctggcgcgga tgtcgaggtc
      181 gtgcagcgtc gagaggtgcg agatcgtcgc gagaccggcg tcggtcgtcg ccgtgtagag
      241 cagcgcgagc acccgcagcg cgcgcagccc actgagccgg tcgagaccgt cgtcggtgat
      301 ctccttgccc gtcagccgaa catcgcgcag gagctggagc ggctcgatca ggtcgaggtc
      361 ggccgacgcc agcggcaggt ggccattctc gaagcgcaga aaccgccgaa ctgggagatg
      421 gaccgtcccg gtcgcctcgc cgagttcctt ccagtagccg tcgtcatcga gcgcgaagag
      481 gtggccgatg gcgcggtcgt cggggaagga cgcctcgtag cggttcgcgg gatcggccag
      541 attgaagcga tcgacgggct ggccatccag acgggccagg ccacggacga cgcgcggatt
      601 ggcctggtcg gtccgcagca ggatgccgga ctggccggtt cggacgagcg cgcgttgatc
      661 ggacatcacg ccctgccgct gaggctgagc tgcttgacca ggtcgtcgta ctggctggcg
      721 agggcgtggc gctccatctg ggctgtcgtc agctcggacc gcaggtcgga ttcgagccgg
      781 tcgagcaggg ccggttcgcg ttcgatacgt tgccagagct gatgcaggct ggtcgagcgc
      841 agtgacgcca gctcgccggt ccgtttggcg agctggccct ccagccggcc gatctcggtc
      901 gttgcccagg cgatccgcgc gccacgggtc gggccgggaa cgtcgtcgtc gccaaccggc
      961 agctcccgct ccagatcgag cagcgtcccg aggtcgtgca tctccatcgc gacattgatg
     1021 cgccgcatga catcctcgtg gcgctcgcgg tgctcttccc cggccacatc ggggtggaac
     1081 ttgcgggcca gcttgcggta ctggctccgg atcgcggcgg cgacttcggg ttcgggcgga
     1141 ggcggcaatt cccgggcgcg gcgtcgggcg ccggatgcct cgcgcgcctc ggcgtcgacc
     1201 cgctcacgct cggcggcaaa ctcacgttcg atggacgact cgacagcccg ccaggcgtcg
     1261 atcgactccc ggagggcggc gatccggcgg cgcaggatgg cgatctccag ctcgacgcga
     1321 tcgacctcga ccatcaggac gccgatccgc gcgtcgtagc gcgactcgaa ggccagcagc
     1381 gtcgaacgta gctgctccag ctcgacgacg gccgccgtga gctgctcgcg caggctgtcg
     1441 atccgggcgc gcagctggtt ctcctcgaca gttgccgggt cgagggcagg cattagctgg
     1501 cggctacgca tgtgttgcgc ccgcctctct cacgctgtcg tgctggatcc ccgattcgcc
     1561 cgccgtcatc atcattcgag tatacgtggc acggcgggcg ggattggcta gcccggttgt
     1621 cctgaatgac gaccagtcac ctggatggca gcacccgcca gcagacttcg gcacgctggc
     1681 cgccgccgac ctggcgcgcg tagcggtcga gcgcggcgcg gtggtctcca agatcgcgga
     1741 agaggaccgg caactggctg gggtagcact cgactgcccg gcacttgcgc tcgaacgcct
     1801 cgtcatccag ccaggtcacg cgcgcctcac ggacggtgcc gcgcgccagc cggtcggtga
     1861 cggcccgctg gttgtcgatg acatatggca cgtcggcgta ggcccagaca gcaatgccgc
     1921 gcgcggcaag gcggcggccg gcccggaacg cgagttgatg atcgacgtgg ttcccggcgg
     1981 ctagcgggac gaaatatttg gcagcgtcca gcgcggcaag ttcggcaacc agcacatcaa
     2041 tcagcagcag gtcatcgtcg tgcagtgtgc cgaacagagc atcgtccgaa gcgtacgctg
     2101 cctgacgata gatcgcgtcg agatggtcga gccagctgtg actgacgcgc tcgccaagag
     2161 cacgggcggc tgcctcgtct tccgcccggc gggatgcggc gaccgacgcg ccatcgatcc
     2221 cccagcgctc gtgctggaag cgggcgaagt cgctgacctg atcggtgggc tgaccgacaa
     2281 agagggtgac gatcgtcggg ctatcggttc gggctgcctg ggcgacggtg ccgccacatg
     2341 acagggcgat gtcgtccggg tgcggggcga cgaagacgat cgatgcagac ggcatcaggc
     2401 gctgccccga acgcgggcat cgccgacgcg gcgggtgatg cgctgctggt cgaagtattc
     2461 gagaacggcc tgggcgtact tgcggctgct gccgaagtgg tcgcggaact gcgccagtgt
     2521 caggctgccg tgctcgtcga tcagccgcag cgtctcgccc tggatctcgg ccagttgg
//
LOCUS       DASUGJ010000132         5162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_17421, whole genome shotgun sequence.
ACCESSION   DASUGJ010000132 DASUGJ010000000
VERSION     DASUGJ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17421"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFV93_02940"
     CDS             <1..351
                     /locus_tag="VFV93_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164328.1"
                     /translation="ADLWNVYFSTTRNSPENIPELNAKVDTACRAAGRDPATLGRTVA
                     AYVDFTDTPGGPTSFNGSRSPALTGSPDEIAAGLRAYADVGIEHVQVHVLPMTATSIE
                     RFAPVLETLDRAKC"
     gene            467..1384
                     /locus_tag="VFV93_02945"
     CDS             467..1384
                     /locus_tag="VFV93_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5164329.1"
                     /translation="MPRPLKIGVELPIAENKGREGTPRWSDISLMARRAEEAGFDSVW
                     FEDHLLFRNEGQPDQGVWDGWSLLAALAAVTERVEIGPLVSCVSFRNPALLAKIADTV
                     DEISNGRLILGLGAGWHEPEYRAFGFQFDHRVSRFEEALKIIHGLLKTGAVDFAGRYY
                     SARECELRPRGPRPEGPPILLGTVGERMLGLTAQYADLWNAYFTHTRNQPELVAPLSA
                     KVDAACQAAGRDPASLGRTAGVYVQMPGTAPNSTPPHWEFTPLAGSPEEMAANLRAYA
                     GQGVSHLILWLEPNTLESIEAFAPVLELV"
     gene            1577..2455
                     /locus_tag="VFV93_02950"
     CDS             1577..2455
                     /locus_tag="VFV93_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02587 family membrane protein"
                     /protein_id="HEX5164330.1"
                     /translation="MVLAQETSGWRRELASELDDFAHGAAGGFLFGIPLLYTMEFWFI
                     GPQISMLHALILLGLSIGISLIFIATIGFREKEPPTWRDILAETIDAIGISLIVTVVT
                     LFMLGKIDFGTPLDITTGTIAIELLPVSLGVAIANHILPRQEDRQGNEEDREAGKQAA
                     GNATTRDVVASAGGALLLSLNIAPTDEVAVLAAELSELQLIGLLIFSLLITYIIVFQA
                     GFRNQRTRLESEGAFQHPLTETIVAYLVALVVSGLTLWIVGALTPPVAFGTILSMTVV
                     LAFPGALGAAAGRLAV"
     gene            2457..2903
                     /locus_tag="VFV93_02955"
     CDS             2457..2903
                     /locus_tag="VFV93_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164331.1"
                     /translation="MAQRDAPDRPDPSSEPPADSGRTTPEKITTAISLLLIVLLAGAI
                     LYEGYWKGQADPATLEVTVRTDQIDQRGGSYYVPVEIHNAGDQTVEEVTVSIDVIDGE
                     NVVQEAETVIATLGEAETVTAVVVLTEDPTGLTIEAGVVTYQIAES"
     gene            3258..>5162
                     /locus_tag="VFV93_02960"
     CDS             3258..>5162
                     /locus_tag="VFV93_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5164332.1"
                     /translation="MRDHALQQQLREAEARFRTLVEEMPAIIYINAWDEPHPMLYVSP
                     HVEEMLGYPAMTWLEQPGFVYSIVHPDDREPLRAENARTEAIGARFSLEYRLIAHDGR
                     IFWVRDECVPVYDIDGYPLFYQGIMIDVSDRHRAEEALRESEERFRGAFEDAAVALGI
                     ATLDGRLLRVNEAHCDLLGYRENELVGTDIRRLIHPDDLASTNQHRADLLAGTIRSYK
                     EERRYLHKDGHIVWGLVSLSLTRDQHGNPLTILAQVQDISDRKLAEEALRESEEYFRS
                     TFDDAGIAMGVADLHLRLLRVNQQYCEMLGYSEDELLALQPSQLMHPDCWDAHERWRG
                     DLLTGRSATQQMERRFRHHDGHVVWGLVNLSLVLGGDGHPRYFLSQIQDITDRKRAEA
                     SLRESEALFRGAFASSMVGISLSTPDGRYIRVNQAFCEMLGYSQSQLLDLTYDAITAI
                     DDLAEGDAYRRRMLAGEIDHFQIEKRYRHAAGHNVWVLLSVAIVRDSHGDPLYVIGQA
                     LDISARKALEDRLTHQAFHDSLTGLPNRALLHDRIAHALERASRNGETIAVMFMDLDG
                     FKAVNDKWGHATGDQLLIAVGARLRGWVRGGDTVARLGGDEFAILREDIRTLDEATRI
                     AARIIAGLETP"
BASE COUNT          931 a         1756 c         1641 g          834 t
ORIGIN      
        1 gccgacctct ggaatgtcta cttctcgaca acgcgcaaca gcccggagaa catcccggaa
       61 ctcaacgcca aggtcgatac cgcctgccgg gcggccggcc gcgacccggc cacgctgggc
      121 cggacagttg ccgcctacgt cgatttcacc gacactcccg gcggaccgac ctccttcaac
      181 ggcagtcgct ccccggccct gaccggcagc cccgacgaga tcgccgccgg gctgcgcgcc
      241 tacgccgatg tcggaatcga gcacgtccag gtccacgtcc tgccgatgac ggcaaccagc
      301 atcgaacgct tcgcgccggt gctggagacg ttggatcgag cgaagtgttg agtgctgagt
      361 gctgagtgct gggtgctgag ttgcggaatc ggctcaggcg cttgacttcc aacaacgatc
      421 gacgtttgcc tttgtcagca ctcagcacta acgacaggag atcgccatgc cacgaccact
      481 caagatcggt gtcgagttgc cgattgcgga gaacaagggc cgcgaaggga cgccgcgttg
      541 gagcgacatc agcctgatgg cacgacgggc ggaggaggcc ggcttcgact cggtctggtt
      601 tgaggaccat ctgctgttcc gcaacgaggg tcagcccgat cagggcgtct gggatggctg
      661 gtcgttgctg gccgcgctgg cggctgtcac cgagcgggtc gagatcgggc cgctggtctc
      721 atgtgtttcg ttccgtaacc ctgcgctgct ggccaagatc gcggacacgg ttgacgagat
      781 ctcgaatggc cggctgatac tcggcctcgg cgccggctgg cacgagccgg agtatcgggc
      841 gttcgggttc cagttcgacc atcgcgtctc gcgcttcgag gaggcgctca agatcattca
      901 cggactgctc aagaccggcg ccgtcgactt cgcgggccgc tactactcgg cccgcgagtg
      961 tgagttgcgc ccgcgcgggc cgcgtccgga ggggccgccg attctgctcg ggacggtcgg
     1021 tgagcggatg ctcgggctga cggcccaata cgccgatctc tggaacgcct acttcaccca
     1081 cacgcgcaac cagccggagc tggtcgcacc actcagcgcc aaggtagacg ctgcctgcca
     1141 ggccgccggc cgcgacccgg ccagcctcgg ccggacggct ggcgtctacg tccagatgcc
     1201 cggcacggcg ccgaacagca cgccgcccca ctgggagttc accccgctgg ccggcagccc
     1261 ggaggagatg gctgccaacc tgcgcgccta cgccggccag ggcgtcagcc acctcatcct
     1321 ctggctcgaa cccaacacgc tggagagcat cgaagccttc gcgccggtgc tggaactggt
     1381 gtgaggagac gggggtgaca ggcagtaggt gataggtgtt aggagctaac ctgtgcggag
     1441 agtcctggca tgctgatccc cacggactaa tccctaacgc ctatcaccta tcacctttta
     1501 cctgttacct cgcacccggc atacgtcgtg cgctgtcgct acccggaggt acggctatgg
     1561 gtgggggatt acgctgatgg tactggcgca ggagacgtct ggctggcggc gggaactggc
     1621 gtcggagctg gacgatttcg cgcatggcgc ggcgggtgga ttcctgttcg ggattccgct
     1681 gctgtatacg atggagttct ggttcatcgg gccgcagatc tcgatgctgc acgcgctgat
     1741 cctgctcggg ctctcgatcg gcatctcgct gatcttcatc gcgacgattg gcttccgcga
     1801 gaaagagccg ccaacctggc gcgacatcct cgccgagacg atcgacgcga tcggcatctc
     1861 gctgattgtg acagtcgtga cgctcttcat gctcggtaag atcgactttg gcacgccact
     1921 cgatatcacg accggaacga tcgccatcga gttgctgccg gtgtcactgg gcgtcgccat
     1981 cgccaaccat atcctgcctc gccaggaaga ccgtcagggc aacgaggagg accgcgaggc
     2041 cggcaaacag gcagccggca acgccacgac tcgtgacgtc gtcgccagtg ccggtggcgc
     2101 gctgctgctc tcgctcaaca tcgcgccgac cgacgaggtg gcggtcctgg cggcggaact
     2161 gagcgagctg caactgatcg gcctgctcat cttctcgctg ctgatcacct acatcatcgt
     2221 cttccaggcc ggcttccgta accagcgaac gcggctggag tcggagggag cattccagca
     2281 tcctctgacc gaaacaatcg tcgcctacct ggtcgcgctg gtcgtctcgg gcctgaccct
     2341 ctggatcgtt ggggcactga cgccaccggt cgcgttcggg acgatcctga gcatgacagt
     2401 cgtgctcgcc ttccccggcg cgctcggcgc ggcagccggc cggctggcgg tctgagatgg
     2461 cgcaacgtga cgccccggac cggcccgacc catccagcga gccgccagcg gacagcggcc
     2521 ggacaacgcc agaaaagatc acgacggcga tcagtcttct gctgatcgtg ctgctggccg
     2581 gcgcgattct ctacgagggg tattggaagg gccaggccga tccagctacg ctggaggtta
     2641 ccgttcgcac cgaccagatc gatcagcgcg gcggcagcta ctacgtcccg gtcgagatcc
     2701 acaacgccgg cgaccagacg gtcgaggaag tcaccgtcag catcgacgtc atcgacggcg
     2761 agaacgtcgt ccaggaggcg gagaccgtga tcgccaccct cggtgaggcg gagacggtca
     2821 cggccgtcgt cgtgttgacc gaagatccga ccggcctgac gatcgaggct ggcgtcgtca
     2881 cctaccagat cgccgaaagc tagccgcgcg tcgcatcgtc cggtcgatcg acgctccctc
     2941 tattgcaaaa catgcgaatg cgcctgtacg ttagattgcg tcttggcaac caccgacagt
     3001 gctaccggcc ttgtccggac aggacgaatt ggtgatcgac gcattcggga ggaacgcggc
     3061 accctacaga gaggttcggg cgcggcaacg gacgccgctc tcagcgtaaa agcgagcaca
     3121 acatcgatca ccgccagcag ttgcaggcac taccaacggc aggcaggcat cacgacaacc
     3181 gaggtgaagt tgaacggcgt gagccaacga cggcgacacg ctcgattggg tttcaggcga
     3241 gatagggtat cgacgcggtg agagatcacg cacttcaaca acagctccgc gaggccgaag
     3301 cgcgcttccg gacgctcgtc gaagagatgc cggcgatcat ctacatcaac gcctgggacg
     3361 aacctcatcc aatgctctac gtctccccgc acgtcgaaga gatgctcggc tacccggcca
     3421 tgacgtggct ggaacagccc ggcttcgtct acagcatcgt ccaccctgac gaccgcgagc
     3481 ccctgcgggc cgagaacgcc cggaccgaag cgatcggcgc ccgcttcagc ctcgaatacc
     3541 ggctgatcgc acacgacggc cgcatcttct gggtccgcga tgagtgcgtg ccggtctatg
     3601 atatcgacgg ctacccgctc ttctaccagg gcatcatgat cgatgtcagc gaccgccacc
     3661 gtgccgagga ggcgctgcgc gaaagcgagg agcgcttccg gggcgccttc gaggatgccg
     3721 ccgtcgcgct cggtatcgcc acactggacg gcaggctgct acgtgtcaac gaggcgcatt
     3781 gcgacctgct cggctaccgc gagaatgagc tggtgggcac cgacatccgc cggctgatcc
     3841 acccggacga tctcgccagc accaaccagc atcgggcaga cctgctggcc ggcacgatcc
     3901 gatcctacaa ggaagagcgc cgctacctcc acaaggacgg ccacatcgtc tggggcctcg
     3961 tcagcctctc gctgacgcgt gaccagcacg gcaacccgct gacgatcctg gcccaggtcc
     4021 aggacatcag cgaccgcaag ctggccgagg aggcgctgcg cgagagcgag gagtacttcc
     4081 gcagcacctt cgacgacgcc gggatcgcga tgggcgtcgc cgacctgcac ctgcgcttgc
     4141 tgcgggtcaa ccagcaatac tgcgagatgc tcggctacag cgaggacgaa ctactcgcgc
     4201 tccagcccag ccagctgatg caccccgact gctgggacgc ccacgagcgc tggcgcggcg
     4261 acctgctgac cggacgctcc gccacccagc agatggagcg tcgctttcgg caccacgacg
     4321 gccacgtcgt ctgggggctg gtcaatctct cgctcgtcct cggcggcgac ggccacccgc
     4381 gctacttcct cagccagatc caggacatca ccgaccgcaa gagggccgag gcgtccctgc
     4441 gcgagagcga ggcgctcttc cgtggcgcgt tcgcatcctc gatggtcggg atatccctct
     4501 cgacccccga tggccgctat atccgcgtca accaggcctt ctgcgagatg ctcggctaca
     4561 gccagtcgca gctgctggac ctgacctacg acgcgatcac cgccatcgac gacctcgccg
     4621 aaggcgatgc ctaccggcgc cggatgctgg cgggggagat cgaccacttc cagatcgaga
     4681 agcgctaccg ccacgccgcc ggtcacaacg tctgggtgct gctcagcgtc gcgatcgtcc
     4741 gcgacagcca cggcgacccg ctctacgtca tcggtcaggc gctcgacatc tccgcccgca
     4801 aggcgctcga agaccggctg acccaccagg cgttccacga cagcctgacc ggcctgccga
     4861 accgggcgct gctgcacgac cggatcgccc acgcgctgga acgcgccagc cgcaacggcg
     4921 agacgatcgc cgtcatgttc atggacctcg acggcttcaa ggctgtcaac gacaagtggg
     4981 gccacgcgac cggcgaccag ctcctgatcg cggtcggcgc acgcctgcgc ggctgggtgc
     5041 gtggcggcga caccgttgcc cggctcggcg gcgacgagtt cgccatcctg cgggaggaca
     5101 tccgcaccct cgacgaagct acccggatcg ccgcacgcat catcgccggc ctggagaccc
     5161 cg
//
LOCUS       DASUGJ010000133         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_156350, whole genome shotgun sequence.
ACCESSION   DASUGJ010000133 DASUGJ010000000
VERSION     DASUGJ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156350"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VFV93_02965"
     CDS             <1..703
                     /locus_tag="VFV93_02965"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5164333.1"
                     /translation="SPRVAVFETARTYQPTTLDELPDERRGVAIAMSGVCAPASWYDG
                     DDGELDFFDVKGAIELMLERLGAKSVAFKPVSHPSMHPGRSAAVCVGDLQVGIAGEIH
                     PRVAAGFDIEGRVAVAEIDLEVFAGTLLENWQVSPVSRFQPIRQDFAVVVDEAVAAGD
                     VRAAIQAGAGPLGTAIELFDIYRGPGIDAGKKSLAFAVTLSAPDRQLAEHEVERIRTK
                     IEQNVKKRVGGTLRS"
     gene            787..>2169
                     /locus_tag="VFV93_02970"
     CDS             787..>2169
                     /locus_tag="VFV93_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HEX5164334.1"
                     /translation="MAGTIDQSSPLNGAARARPVERVRGTHDRLAGELAGAEHLRQTL
                     TGNAARFGYQPVDTPVLEHTDLFLRKSGGDRIAQLYAFEHRGRGLALRPEFTAPAVRL
                     FIEHMQGEPLPVRLSFSGPVFRYEKPQSGRSRQFTEFGCELIGASGPVADAEIIGLAL
                     DGLRLAGVAQPKLVLGHIGVVINFLGGLDIDRRAQDWLTWSMERLRKGDDSAHELPPH
                     LQRLEASLDETPDLPDSEDEIDAEAIASILRQAGVEFQGGSREPEDIVRGLFAKRRRR
                     YDAHVLREAAGFVSELTRLVGPPDDVLQPLRDLVAANGLDQTPLDELEKVIELLAATG
                     FDRSDVWLDLGMGRGLHYYTGMLFEIYAGAVDGPQLCGGGRYDDLAQVLGARQPVAAC
                     GFSYGLERVLAAGAVCDLPDQHPAVLVLQGDDPAGALALADGFRAAGWQANVDLRERN
                     QSATRRVAQRQ"
BASE COUNT          346 a          696 c          768 g          359 t
ORIGIN      
        1 cagcccgcgc gtcgcggtat tcgagacggc gcgcacctat cagccgacca ccctggatga
       61 actaccggac gagcgccgtg gtgtcgccat cgcgatgagc ggcgtctgcg cgccagccag
      121 ctggtacgac ggtgatgacg gcgagttgga tttcttcgac gtcaagggcg cgatcgagct
      181 catgctcgaa cggctcggcg cgaagagcgt cgcgttcaag ccggtcagtc atccgtcgat
      241 gcaccctggc cgaagcgccg ccgtctgcgt cggtgatctc caggtgggta ttgccggtga
      301 gattcatcca cgggtggccg ccggtttcga catcgaaggt cgcgtcgcag tcgccgagat
      361 cgaccttgag gtgttcgccg gtacgctgct ggagaactgg caggtgtcgc ccgtctcgcg
      421 cttccaacca atccggcagg acttcgccgt cgtagtcgac gaggcagtcg cggccggtga
      481 cgttcgggct gcgattcagg ccggcgcggg tccgttggga acagccatcg agctgttcga
      541 tatctatcgc ggaccgggga tcgacgccgg gaagaagagc ctggcgttcg ccgtgacgct
      601 ctcggcgccc gaccggcagc ttgccgaaca cgaggtcgag cgcatccgca ccaagatcga
      661 acagaacgtg aagaagcgtg tcggcggcac actgcgttcc tgatatgcga ttctttagtg
      721 gttaggcgag cgcgcctgcg ctgcacgttg cagcgttggc gcgttgtgaa agagataagc
      781 gggttcgtgg caggaacgat cgatcaatcg tcgcccctga acggcgcggc cagagcgcgg
      841 ccggtggaac gcgtgcgcgg gacgcacgac cggctggccg gtgagctcgc gggcgcggag
      901 cacctgcgcc agacgctgac cggcaacgcg gcgcggttcg gctatcagcc ggtcgacaca
      961 cctgtcctgg agcacactga tctgttcctg cgcaagtcgg gcggggaccg gatcgcccag
     1021 ctctacgcgt tcgagcaccg aggtcgcggg ctggcgctgc ggccggagtt cacggccccg
     1081 gccgttcgct tgttcatcga gcacatgcag ggcgagccac tgccggtgcg actgtcgttc
     1141 agcgggccgg tcttccgcta tgagaagccg cagtcgggtc ggtcgcggca gttcactgag
     1201 ttcggctgcg agctgatcgg cgcctccgga ccggtcgccg atgccgagat catcggcctg
     1261 gcgctcgatg gcttgcggct ggctggtgtc gcacagccca agctcgtcct gggccacatc
     1321 ggggtcgtca tcaacttcct cggcgggctg gatatcgacc gtcgcgcgca ggactggctg
     1381 acgtggagta tggagcggct acgcaaaggc gacgacagcg cccacgaact gccgccacac
     1441 ctccagcggc tcgaagcctc gctcgacgag acacccgacc tgccggacag tgaggacgag
     1501 atcgacgccg aagcgatcgc gtcgatcctg cgccaggcgg gagttgagtt tcagggcggc
     1561 tcgcgtgagc cggaagacat cgttcgcgga ctgttcgcca agcgccggcg acgctacgac
     1621 gcccatgtcc tgcgcgaggc ggccggattc gtcagcgagc ttacccggct ggtaggaccg
     1681 ccggacgacg tgttgcagcc gctgcgagac ctcgtcgcgg ccaacggcct ggaccagacg
     1741 ccgctcgatg aactggagaa ggtgatcgag cttctggcgg cgaccgggtt cgaccgttcc
     1801 gatgtctggc tcgacctcgg aatggggcgg ggattgcact actacaccgg catgctgttc
     1861 gagatctacg ccggcgcggt cgacggcccg caactctgcg gtggcggacg ctacgacgac
     1921 ctggctcagg tcctcggcgc gcgccagccg gtcgccgcct gtggcttctc ctatggcctg
     1981 gagcgcgtcc tcgcggccgg cgcggtatgc gatctgcccg accagcatcc ggccgtgctg
     2041 gtgttgcagg gcgacgaccc ggccggcgcg ctggcgctcg cggacggatt cagggcagcc
     2101 ggctggcagg cgaatgtgga cctgcgcgag cgcaaccagt cagcaaccag gcgtgtcgcg
     2161 caacggcag
//
LOCUS       DASUGJ010000134         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_573072, whole genome shotgun sequence.
ACCESSION   DASUGJ010000134 DASUGJ010000000
VERSION     DASUGJ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573072"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFV93_02975"
     CDS             <1..262
                     /locus_tag="VFV93_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5164335.1"
                     /translation="ANGHASEIGTSIMLHLEPEMVVRDRIKDQTPTGSVPYADITQYK
                     GMKARSDSGVMGNPEKGTAEKGAQIASRAVERIVAFVKDELS"
     gene            362..1048
                     /locus_tag="VFV93_02980"
     CDS             362..1048
                     /locus_tag="VFV93_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164336.1"
                     /translation="MAPRPGFKVHKSFERADPALVEQFRGLASANISDIQGRQQTFDA
                     RIKPVYSPMAPLCGPAFTVKARPGDNLLATKAIHMAQPGDVLVISGGFEMNLSVWGGV
                     MSAIAKAKGIAGVITDALVRDVAEIRDVGLVVFATGLTPVGPTKEGVGQINTPIVCGG
                     VIVNPGDIIVGDEDGAVVVRKEEAEAVLQRTHARMELEKNWFEAIARGETILEDSDDY
                     LRERGAEIID"
     gene            1163..1948
                     /locus_tag="VFV93_02985"
     CDS             1163..1948
                     /locus_tag="VFV93_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5164337.1"
                     /translation="MVKTRLAQMTWKEAEEAYKANPVILIPSASIEQHGPATPVGDFR
                     ISEWVGDRIAERTGAIVAPMLPYGYSEVFRRFPGTVTLQGDTVRAVMYDVVVSFAEQG
                     LDHLVFLCGHNGNMPILDQVARRVRREYGIRMGCIEPFRLFSGATMAEAYGKPGVAIG
                     HGSDPIMSFNMFLFPDDCRQDLTEAENLNQYDGMTIRGSTNVALGDVAGHIYFDTLDI
                     APNGVMGDVSNFDAEAGGKLLAHVVDKGVEFVNIFKQHDTRIR"
     gene            1984..>2277
                     /locus_tag="VFV93_02990"
     CDS             1984..>2277
                     /locus_tag="VFV93_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164338.1"
                     /translation="MEERYRPADDEQDLGIKAALGGAVSRRQLLKRAALLGLSAPVIA
                     GLLAACGGDDDDDDDDDGGATSTTASGGNATAPAGQTPSGGGAASPTSGGSTGS"
BASE COUNT          439 a          731 c          750 g          357 t
ORIGIN      
        1 ggccaacggc cacgcctcgg agatcggcac ctcgatcatg ctccatctcg aacccgagat
       61 ggtcgtccgc gaccggatca aggaccagac gccgaccggc agcgtcccct acgccgacat
      121 cacccagtac aagggcatga aagcccgctc cgactccggc gtcatgggca acccggaaaa
      181 aggcaccgcc gagaagggcg ctcagatcgc gtcccgcgct gtcgaacgga tcgtcgcgtt
      241 tgtgaaggat gagttgagct agacgtcgtg acacgtgacg cgtgatgcgt cacgcgagga
      301 ctggaaccaa accgcgtcac gcatcacgcg tcacgcgtca cgaaacacga ggggaacacg
      361 aatggcacca cgaccagggt tcaaggttca caagtcgttc gagcgggctg atccggcgct
      421 ggtcgagcag ttccgcgggc tggcgtcggc caatatctcc gacatccagg gacggcagca
      481 gacgttcgac gcgcggatca agccggtcta ttcgccgatg gcgccgctct gtggcccagc
      541 cttcaccgtc aaggcacggc ccggtgacaa cctgctggcg acgaaggcga tccacatggc
      601 ccagccgggc gacgtgctcg tcatctccgg cggcttcgag atgaacctgt ccgtctgggg
      661 cggcgtcatg agcgcgatcg ccaaggcgaa ggggatcgcc ggcgtcatca ccgatgcgct
      721 cgtccgtgac gtggccgaga tccgcgatgt cggcctggtc gtcttcgcga ccggcctgac
      781 gccggtcggc ccgaccaagg agggtgtcgg ccagatcaac acaccgatcg tctgcggcgg
      841 ggtcatcgtc aaccccggcg acatcatcgt cggcgatgag gacggcgccg tcgtcgtccg
      901 caaggaagag gccgaggccg tcctccagcg cacccacgcc cggatggagc tggagaagaa
      961 ctggttcgaa gccatcgccc gtggcgaaac gatccttgaa gactcagacg actacctgcg
     1021 cgagcgcggc gcggagatta ttgactgatc gggactcggg attcgggatt cgggggcttg
     1081 aaggggcgtg ttctgttcat tgcccccaaa tccccaatcc ccaatcccga cctacatgct
     1141 tcctgaacga gaggagcgga ttgtggtcaa aacccgactc gcccagatga cgtggaagga
     1201 ggcggaggag gcgtacaagg ctaacccggt gatcctgatt ccgagcgcgt caatcgagca
     1261 gcatgggccg gcgacgccgg ttggcgactt ccggatcagc gagtgggtcg gcgaccggat
     1321 cgccgaacgg accggcgcca tcgtcgcgcc gatgctgccg tatggctact cagaagtctt
     1381 ccgccgcttc cccggcaccg tcacgctcca gggcgacacc gtcagggccg tcatgtacga
     1441 cgtggttgtc agcttcgcgg aacaggggct ggatcacctc gtcttcctct gcggccacaa
     1501 cggcaacatg ccgattctgg accaggtcgc gcgtcgggtg cgccgcgagt acgggatccg
     1561 gatgggctgc atcgagccgt tccggctctt ctcgggggcg acgatggccg aggcatacgg
     1621 caagccgggc gtcgcgatcg ggcacggcag cgatccgatc atgtcgttca acatgttcct
     1681 cttccccgac gattgccggc aggatctgac cgaggcggaa aacctgaacc agtacgatgg
     1741 catgacgatt cgtggcagca ccaacgttgc gctgggcgat gtcgcgggcc acatctattt
     1801 cgacacgctc gacatcgcgc cgaacggcgt gatgggcgat gtttccaact tcgatgccga
     1861 ggccggtggc aaactactgg cccacgttgt tgacaaaggc gttgaattcg tcaacatctt
     1921 taagcaacac gatacccgga ttcggtaacg cgatcgatcc cgtagtgagg agaggacatc
     1981 accatggagg aacggtatcg accggctgac gatgaacagg atctgggaat caaggccgcg
     2041 ctcggcggcg cggtctcccg acggcaactg ctgaagcggg cggccctgct cggcctgagc
     2101 gcgccggtca tcgccggcct gctggccgcc tgcggtggcg acgacgacga tgacgacgat
     2161 gacgacggtg gcgcgaccag caccaccgcc agcggcggga acgcgactgc cccggccggc
     2221 cagacgccgt ccggcggtgg cgccgccagt ccgaccagcg gcggcagcac cggcagc
//
LOCUS       DASUGJ010000135         7615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_851667, whole genome shotgun sequence.
ACCESSION   DASUGJ010000135 DASUGJ010000000
VERSION     DASUGJ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851667"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..913)
                     /locus_tag="VFV93_02995"
     CDS             complement(131..913)
                     /locus_tag="VFV93_02995"
                     /inference="COORDINATES: protein motif:HMM:NF009499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5164339.1"
                     /translation="MNDSSTDRRVAIVTGASRRGAIGAAICRRLAEAGVDICFTYWAA
                     YDREMPWGSDDDGPAALEQELTAIGVRAAALEADHARPETATAVLDGATARLGQPSIL
                     VNNAAYSTSEGYEDLDAATLDAHYAVNLRGMALLSAEFARRWPGGPGGRIINLTSGQS
                     QGPMPGELAYVATKGAVDAFTLSLSAGVFAKGITVNAVNPGPTDTGWMSPDLKALLVT
                     KFPTGRIGQPDDVARLVAFLASPEAEWITGQVIHSEGGFLRV"
     gene            1139..1759
                     /locus_tag="VFV93_03000"
     CDS             1139..1759
                     /locus_tag="VFV93_03000"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX5164340.1"
                     /translation="MDRELQRQRAAQFRAQRERYLQTRQQRLQPALISNRKLISTRHR
                     HLPALLVAPFALLAAAGSLDLLFLVTGNGFWTLLSFTMIPAGIVGCVVSAAIGLHDWF
                     IAPPGSRVRGFGIWFTIGAALVVAVFTQSWVARFGIADDAGGLGVVLGFMGAALALLT
                     GLLLGEYLDRLAALTPAEAPEPAVVDQIATPQRVEEIIEQRQVAHA"
     gene            1855..3258
                     /locus_tag="VFV93_03005"
     CDS             1855..3258
                     /locus_tag="VFV93_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5164341.1"
                     /translation="MDRAAFLEHFTSLNEGLDAWHRGSGPFDADGSVAVPAEKLTAVF
                     SELSGRLRDNYPYGHPRYAGQMLKPPHPVAVLAYATAMQINPNNHALDSSMATSRMER
                     EAVAALARMFGYDATHLGHLTGGGTIANLEALWVARSLHPDTAIAVSDQAHYTHARAC
                     ELLGARLVSIPSDERGRIDLAALRDALKSGEIGTVVATTGTTGLGAVDPVHEIVPLAR
                     AAGARVHVDAAYGGFFSLLARRDNPPIDPAPFLAIAESDSVVVDPHKHGLQPYGCGSV
                     LFRDPAIGRFYVHDSPYTYFTSDELHLGEISLECSRAGAAAAALWATLEALPLDPSDG
                     FGAILAQARAAALDWAALIRDSAQLRLVVEPELDIINFYPVPRRPGSVCASQISALTD
                     RVFRILMDDPNDPIFLAKLIVQRDQLAGRHPSIEWDTGSMTVLRSVLMKPEHRAWVPR
                     LHRAVEAALATAEMEST"
     gene            3260..3856
                     /locus_tag="VFV93_03010"
     CDS             3260..3856
                     /locus_tag="VFV93_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164342.1"
                     /translation="MRERIEIGPVVRVQVQLSSLKAGPPKGRYFDPAPLQQAEWLDLT
                     SDGATTEHGGECAIDVHNSTHPATKNRDGINPISIGFTSHYQRMRERFDTHLTNGIAG
                     ENILVETDEMITLGELADGLVIEGGDGRQIELSRVSVAHPCVEFSRFALGDRSAPPLA
                     VSEALRFLDNGLRGFYVAVESSRPLRVAPGDRVFALRR"
     gene            3882..4766
                     /locus_tag="VFV93_03015"
     CDS             3882..4766
                     /locus_tag="VFV93_03015"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5164343.1"
                     /translation="MPRPEERGVPSNWGRWGEEDQRGTANLLSDEGVLAASQLVGRGK
                     VYSLAAPLSPNGPNLPTRRPTWHVVTHGRRASGGDDMSADDVVMMHTHGTTHIDALCH
                     IFVGDTLYNGHPAQTLTPYGAQKCGIDNVGPIVGRGVLLDMATFRDRQRLESGEPITP
                     DDLDACARAEGVEIRPGDIVLVRTGWWQLHSEGVEARKTFYNGEPGPDGDCGNWFQAR
                     DIVALGADNPAVEAVASWRDPLPVHRAVIWGCGGYLLEFLDLEALARDRAYEFMFVAT
                     PLRLEGGIGSPIVPLAIV"
     gene            4813..5769
                     /locus_tag="VFV93_03020"
     CDS             4813..5769
                     /locus_tag="VFV93_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5164344.1"
                     /translation="MVSIADIWAARPIVMEAAIRTPLVRLRVEDAPAEIYLKLENLQP
                     IGSFKLRGAWNAMRHMSARELDRGVLTASAGNMAQGVAFAAQRFSVPCTVIAPDNAPA
                     AKEQAVLRMGAEFRRVPRDDWWQTFQQRSFPGLEGRTFVHAFDDDLVMAGNGTIALEL
                     LEDLPDVDTVVVPFGGGGLASGIATGLRALKPDVKIYAAEVDVAAPLAASLAAGEAQT
                     IEYGSSFVDGIGSTMVFPKMWDLASNLLDGSHTATVAQAEDAVRLLAERQRVVAEGAA
                     ACALAVALSGQAGTGKIACIISGGNIDTDVLTDILNRGREAG"
     gene            5958..6152
                     /locus_tag="VFV93_03025"
     CDS             5958..6152
                     /locus_tag="VFV93_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164345.1"
                     /translation="MQSTVSNKPISIAQLRREGFTASQIRQLKALRAAYPVREHVTSS
                     QDLHRLELLRWMRQNGRLED"
     gene            complement(6165..7046)
                     /locus_tag="VFV93_03030"
     CDS             complement(6165..7046)
                     /locus_tag="VFV93_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5164346.1"
                     /translation="MIAEIHSGGLIANALSDELEGLAARVQAGVVEISDGRGTGAGTN
                     WSSDGLIVTNHHVVPGESARVGFTDGRVLDARVVSSLPERDLTLLKVEAIDLPALPVG
                     DSAVLRPGEIVLAVGHPHGIPSVVTFGIFSRFGPYEGDGRVPKHLREGLLADIELRPG
                     NSGGPLVNANGEVVGINAMVLGPGTAMAVPSATVRRLLAAGERRTLGVQIGVVNAGAT
                     SQQEPHLMVLDVVPASEAARAGLLPGDMLLAIGGQALEEPGDIALALREASAAESLSL
                     TIQRAGRRLDVLVAPAG"
     gene            7260..>7615
                     /locus_tag="VFV93_03035"
     CDS             7260..>7615
                     /locus_tag="VFV93_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164347.1"
                     /translation="MTTREQPRESPATAEHARLAGNREVVAGWRRWGPYVSDRAWGTV
                     REDYSANGDAWNFLSHDHARSKAYRWGEDAIAGLCDRYQILVFAPAFWNGVDPILKER
                     LFGLIPGEGNHGEDVK"
BASE COUNT         1268 a         2716 c         2406 g         1225 t
ORIGIN      
        1 ggatcggttc gtcgaaggca accttgatgg ccagttcgaa tccggccacc tgaccgccac
       61 ttcccgccgt gccgaggctc tgctgcgcga catgggcgac tgaccgcgtc gtgcccccgt
      121 cgtcaaccgt tcagacacgc agaaagccgc cttcggagtg aatgacctgg ccggtgatcc
      181 attcggcttc ggggctggca aggaaggcga ccagccgcgc gacgtcatcg ggttggccga
      241 tccggccggt ggggaacttc gtcaccagca gggctttcag gtccggcgac atccagccgg
      301 tgtcggtcgg gccggggttg acggcgttga cggtgatccc cttcgcgaaa acgccggccg
      361 agagcgagag ggtgaaggcg tcgacggcgc ccttcgtcgc gacgtaggcc aactcgccgg
      421 gcatcggccc ctgcgattgg ccagaggtca ggttgatgat ccggccgccc gggccgcctg
      481 gccagcggcg ggcaaactct gccgagagca gcgccatccc gcgcaggttg accgcgtagt
      541 gcgcgtcgag cgtggccgcg tcgaggtcct cgtagccctc actggtcgaa taggccgcgt
      601 tgttgaccag aattgacggc tggcccagcc gcgcggtcgc cccatcgagg accgccgtcg
      661 ccgtctccgg ccgcgcgtgg tcggcttcga gcgctgcggc ccgcacaccg attgccgtca
      721 gctcctgttc gagcgcggcc gggccatcgt cgtcactgcc ccacggcatc tcacggtcgt
      781 aggccgccca gtaggtgaag cagatgtcga cacctgcctc ggccaacctg cggcagatcg
      841 ccgccccgat cgcgcctcgg cggctggcgc cggtcacgat cgccacgcgc cggtccgtgc
      901 tcgagtcgtt catcgacgtt ctccaattcg atctgcagcc gggaaccagt tcgctggttc
      961 cggcgttact tcttcaacga acgtgcggcg gcgcaattta ctgtggcgtc cgtcttgcga
     1021 agagaaaatg acacattgcc acgctcttat ggagcggggt catccgtcgg cgcacgacat
     1081 cgcggtcgca cttgtcacta cctgcgcgcc ggaagacaac cgaacgggag ggattccgat
     1141 ggaccgcgag cttcaacggc aacgcgccgc ccaattccga gcacaacgcg aacgctacct
     1201 gcaaacgcgc caacaacgat tgcagccggc gctgatctcc aaccgcaagc tcatctccac
     1261 ccgccaccgt cacctgcccg ccctgctggt cgcgccgttc gcgctgctgg ccgccgccgg
     1321 atcgctcgat ctgctcttcc tcgtcaccgg caacggcttc tggacactgc tcagcttcac
     1381 gatgataccg gccgggatcg tcggctgcgt cgtctcggcc gccatcggcc tgcacgactg
     1441 gttcatcgcc ccgcccggct cccgcgtgcg tggcttcggg atctggttca cgatcggcgc
     1501 ggcactggtc gtcgccgtct tcacgcagtc atgggtcgca cgcttcggca tcgccgacga
     1561 tgccggcggc ctcggcgtcg tactcggttt catgggcgcc gctctcgcgc tgttgaccgg
     1621 cctgctgctc ggcgaatacc tcgaccgtct ggccgcgctc acgccagccg aagcacccga
     1681 gccggccgtc gtcgatcaga ttgccacccc acagcgggtc gaagagatca tcgagcagcg
     1741 ccaggtcgcg cacgcctgag acagcgacat ccaccgtcat ctcgcaatcc tgtaccccgt
     1801 cttcggacat gtgcggttac actgtgactc gcgatggacg gagaggggag agcggtggac
     1861 cgcgcggcgt ttctggagca tttcaccagc ctgaacgagg ggctggacgc ctggcaccgt
     1921 gggtcgggac cgtttgacgc cgacggcagc gtcgccgttc cggccgaaaa gctgaccgcc
     1981 gtcttcagcg agctctccgg gcgcctgcgc gacaattatc cctacggcca tccccgctat
     2041 gccgggcaga tgctgaagcc gccacacccg gtcgccgtcc tcgcctacgc cacggcgatg
     2101 cagatcaacc cgaacaatca cgcgctcgac agcagcatgg cgacctcgcg gatggagcgc
     2161 gaagccgttg ctgccctggc gcgcatgttc ggctacgacg ccacacacct cggtcacctg
     2221 accggcggcg ggacgatcgc caacctcgaa gccctctggg tggcccgctc gctccatccg
     2281 gacacggcaa tcgccgtcag cgatcaggcg cactacaccc acgcccgcgc ctgcgagctg
     2341 ctcggcgctc ggctcgtgtc gatcccgtcc gacgagcgcg ggaggatcga cctggccgca
     2401 ctccgcgacg cactcaaatc cggcgagatc gggaccgtcg tcgcgacgac cggcacgacc
     2461 ggcctcggcg cggtcgatcc cgtccacgag atcgtcccgc tggcacgcgc cgccggggcg
     2521 cgggtccacg tcgacgccgc ctacggtggt ttcttcagcc tgctggcccg gcgcgacaac
     2581 ccgccgatcg acccggcccc gttcctggcg atcgccgagt cggatagcgt ggtcgtcgat
     2641 ccacacaagc acggtctcca gccgtacggc tgcggctcgg tcctgttccg cgatcccgcc
     2701 attggccgct tctatgtcca cgattcgccg tatacctact tcacgtccga cgagctgcac
     2761 ctcggcgaga tcagcctgga atgctcgcga gccggcgctg ccgccgccgc cctctgggct
     2821 acgctcgaag cgcttccgct cgatccatcg gacggattcg gcgcgatcct cgctcaggcg
     2881 cgagccgccg ccctcgactg ggccgcgctg attcgcgaca gcgcgcagtt gcggctggtt
     2941 gtcgagccgg agctggacat catcaacttc tatcccgtcc cacggcggcc gggttcagtt
     3001 tgcgccagtc agatcagtgc cctgaccgat cgtgtcttcc gtatcctgat ggatgatccg
     3061 aacgacccga tcttccttgc caagctgatc gtgcaacgtg accagctggc cgggcgacat
     3121 ccgtctatcg agtgggacac cgggtcgatg acggtcctgc gcagcgtcct gatgaagccc
     3181 gagcaccgtg cctgggtgcc tcgcctgcat cgcgccgtcg aagcagcgct cgcaacagcg
     3241 gaaatggagt cgacgtaacg tgcgtgaacg gattgagatc gggccagtcg tccgcgttca
     3301 ggtccagctg agcagcctca aggccgggcc gccgaaggga cgctacttcg acccggcgcc
     3361 gctccagcag gccgagtggc tcgatctgac cagcgacggc gcgacgacgg aacacggcgg
     3421 cgagtgcgcg atcgacgtcc acaactccac ccacccggcg accaagaacc gcgacgggat
     3481 caacccgatc tcgatcggct tcaccagcca ctaccagcgc atgcgcgaac ggttcgacac
     3541 gcatctgacg aatggcatcg ccggtgagaa catcctcgtc gagaccgatg agatgatcac
     3601 gctcggtgaa ctggcagacg gactcgtgat tgagggcggc gacggccgcc agatcgaact
     3661 gtcacgcgtc agcgtcgccc atccatgcgt cgagttcagt cgcttcgcgc tcggcgatcg
     3721 atcagcgccg ccactggctg tctctgaggc gctgcgcttc ctcgacaacg gcctgcgcgg
     3781 cttctacgtc gccgtcgaat cgagccggcc gcttcgcgtc gctcccggcg accgcgtctt
     3841 tgcacttcgt cgttagtcat gagccagaaa ggggccacac catgccacga ccagaagaac
     3901 gcggcgttcc cagcaactgg ggccgctggg gcgaggaaga tcagcgcgga accgccaacc
     3961 tgctgtccga cgagggcgtt ctggccgcct cccaactcgt cggcaggggc aaggtctact
     4021 cgctggccgc gccactctcg ccgaacggcc cgaacctgcc gacccgccgc ccgacctggc
     4081 acgtcgtcac ccacggccgg cgcgcatcag gtggcgatga catgagcgcc gacgatgtcg
     4141 tgatgatgca cacgcacggc acgacccaca tcgacgcact ctgtcacatc ttcgtcggcg
     4201 acacgctcta caacggacat ccagcccaga cgttgacccc gtacggtgcc cagaagtgcg
     4261 gcatcgacaa cgtcggaccg atcgtcggcc gcggcgtcct gctggatatg gcgaccttcc
     4321 gggaccggca acggctcgaa tcaggcgagc cgatcacccc cgacgacctg gacgcctgtg
     4381 ccagggccga gggcgtcgag atccgacccg gcgacatcgt cctcgtccga accggctggt
     4441 ggcagctgca tagcgagggc gtcgaggcac gcaagacgtt ctacaacggc gagccggggc
     4501 cggacggcga ctgtggcaac tggttccagg cacgggacat cgtcgcgctg ggagcggaca
     4561 acccggccgt cgaggcggtc gcgtcctggc gcgatccact gcccgtccat cgcgccgtca
     4621 tctggggctg cggcggctac ctgctcgaat tcctcgatct ggaagcgctc gcccgcgacc
     4681 gtgcctacga gttcatgttc gttgcgacgc cgctacgcct cgaaggtggc atcggcagcc
     4741 cgatcgttcc gctcgcaatc gtctaaccag caggtgttcc ggttctccag ttcagagtga
     4801 gaggcatagt tgatggtatc gatcgcagac atctgggcgg cccggccgat tgtcatggag
     4861 gcggccatcc ggacgccgct ggtacggctg cgtgtcgaag atgcgccggc cgagatctac
     4921 ctcaagctgg agaacttgca gccgatcggc tcgttcaaac tgcgcggcgc ctggaatgcg
     4981 atgcgccaca tgtcggcacg cgaactcgat cgcggcgtcc tgacagcctc ggctggcaac
     5041 atggcccagg gcgtcgcctt cgcggcccag cgcttcagcg tgccatgcac cgtcatcgcg
     5101 ccggacaacg cgccggccgc caaggagcag gctgtcctgc ggatgggcgc tgagttccgc
     5161 cgtgttcctc gggacgactg gtggcagaca ttccagcaac gcagtttccc cggtctggag
     5221 ggacgcacct tcgtccacgc ctttgacgac gatctcgtca tggccggcaa cgggacgatt
     5281 gcgctggagc tgctggagga cctgcctgac gtcgacaccg tcgtcgtgcc gtttggcggc
     5341 ggcgggctcg cctccgggat cgcgaccggc ctgcgggcgc tcaaacccga cgtaaagatt
     5401 tacgccgccg aagtcgatgt cgccgcgcca ctggccgcct cgctagcggc cggcgaagcg
     5461 cagacgatcg agtacggatc gtcattcgta gacggtatcg gcagcacgat ggtcttcccg
     5521 aagatgtggg atctggccag caacctgctc gacgggtcgc atacggccac agtcgcccag
     5581 gccgaggacg cagtacgcct gctggccgag cgccaacgtg tcgtcgccga gggcgccgcc
     5641 gcctgcgcgc tggccgtcgc cctctccggc caggccggca ccggcaagat cgcctgcatc
     5701 atctccggcg gcaacatcga caccgacgtc ctgaccgaca tcctcaatcg cggccgggaa
     5761 gcaggataac cacctgtcgt ccaccgccca gaggcccttc gctgccgggt gccctctggg
     5821 cggtggacga caggtgtcct ctgtatcgct caacacacgt tcagcgtgtc gcgctgcgct
     5881 gacgcgtgcg ccggcttccg tcaggatgga cgcatgatcg atcgcggtca gcagccagcc
     5941 tgagaggagc cgaaccaatg cagtcaaccg tcagcaacaa accgatctca attgctcaac
     6001 tacggcgtga gggtttcaca gccagccaga tccgccagct caaggcgctg cgggcggcct
     6061 acccggtccg cgagcacgtc acctcgagcc aggacctgca ccggctggag ctgctgcgct
     6121 ggatgcgcca gaacggccgg ctcgaagact gaccggcgcc cgcctcaacc ggcaggcgcg
     6181 accaacacgt ccagacgccg gccggctcgc tggatcgtca atgacagcga ctcggccgcc
     6241 gacgcctcgc gcagtgcgag ggcgatgtca cccggctctt cgagcgcctg accgccgatc
     6301 gccagcagca tgtcgccggg cagcagaccc gccctggcag cctcagacgc cgggacgacg
     6361 tccagcacca tcaggtgcgg ttcctgctgc gacgtcgcac ccgcgttcac gacgccgatc
     6421 tgtacaccca gcgtgcgccg ctcaccagcc gccagcaacc gccgaacggt cgcgctcgga
     6481 acggccatcg ccgttcccgg ccccagcacc atcgcgttga tgccgaccac ttcgccgttt
     6541 gcgttgacta gcgggccgcc cgagttgccc ggccgcaact cgatatcggc cagcagcccc
     6601 tcgcgcaggt gcttcggcac ccgtccatcg ccctcgtacg gcccgaaccg gctgaagatc
     6661 ccgaacgtca cgacgctggg gatgccgtgc ggatgcccaa cggcgaggac gatttcgccc
     6721 ggacgcagga cagccgagtc gccaaccggc aacgccggca ggtcaatcgc ctcaaccttc
     6781 agcagggtaa gatcccgctc cggcaggctg ctcaccaccc gtgcatcaag cacccgccca
     6841 tcggtgaagc ccacgcgggc cgactcgccc ggcacgacgt ggtggttcgt gacgatcagg
     6901 ccgtcgctgc tccagttcgt cccggcgccg gtaccccggc cgtcactgat ttcgaccacc
     6961 ccggcctgga ccctggcagc caaaccctca agctcatccg aaagcgcatt cgcgattagc
     7021 ccgccgctgt gaatctctgc tatcactcgt gtctccgtct atgctggcgc gcgcccaggg
     7081 ggcggcacag gtgcgcgaac accggccaat cgtgctaccg tactcaacgt tcggcagtca
     7141 tccgttctcc aatcctgccg caggcacgat cggggtgcat cagtgaacca gtcaggtcgg
     7201 caccagccat ttgggcaggt cgcgcagcca ctacgaccgg cggcgcctgg gcggcgagcg
     7261 tgacgacgcg agaacagccg cgcgaatccc cagcgaccgc tgaacacgcg cggctggctg
     7321 ggaatcggga ggttgtcgcc ggctggcgac gttggggtcc atacgtctct gaccgcgcct
     7381 ggggtaccgt ccgtgaggac tacagcgcca acggcgacgc ctggaacttc ctcagtcacg
     7441 accacgcccg cagcaaggca taccgctggg gcgaggacgc gatcgcgggt ctctgcgacc
     7501 gttatcagat tctggtcttc gcgccggcct tctggaacgg ggtcgatccg atcctgaaag
     7561 agcggctgtt cgggctgata ccgggcgagg gcaaccacgg cgaggacgtc aagga
//
LOCUS       DASUGJ010000136         6011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_17653, whole genome shotgun sequence.
ACCESSION   DASUGJ010000136 DASUGJ010000000
VERSION     DASUGJ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17653"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1105
                     /locus_tag="VFV93_03040"
     CDS             116..1105
                     /locus_tag="VFV93_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5164348.1"
                     /translation="MRFSLRLNNDLPIKEYVRLAQAAEEAGFDQFWVSHDLFLRSSPA
                     ILTAVALATERIELGTCILNPYTLHPAELAMVAATLDEVSEGRFNLGLGAGAAAFMKW
                     VRLPYERPLATTRRAIQQLRAVLDGEQPPGWEPEAYLRFDSGSGRRIPIYLAALSPYM
                     LRLAGELADGVLPLLFPPEHYATVEPLIREGAERAGRSMAEIDLAACVWCSISDDRQA
                     AENVLRDKIAYYGHALSPLIFDRLGLTAGDFRATERALMTERDPLKARALVTDRMLRI
                     GIVGTPADLVARLESLVALGVRHLSFGPPLGPDPLAAVRAIGAQLPAVRDWSS"
     gene            1249..2322
                     /gene="coxB"
                     /locus_tag="VFV93_03045"
     CDS             1249..2322
                     /gene="coxB"
                     /locus_tag="VFV93_03045"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5164349.1"
                     /translation="MDRRFPGRWPGGFRSGRLFRILAVVTLLLLAFTLSACSVTSDPQ
                     STIARDGRANDRIWFVYDILWIGVAVVLILVEGLLIYTLIRFRRRPKTVHGRPVPVHG
                     NTTLEIAWTIIPAVILVAISIPTLQVLADLSDPPDDQPQMQIDVIGHQFYFEFQYPEE
                     GLQTTNTLHIPEDTIVDIDITSGDVIHSLWVPRLNGKTDAVPGRTNDMWIEADNPGTY
                     HGQCAEFCGTGHALMRFTVEVHTKADYDAWVQQQKDRAAGGGELAQRGQQIAQENGCT
                     GCHSIDGSAAVGPTWQGLFGHEVTLSDGSTVTADEAYITESIRDPSAKIVQGFPDIMP
                     KTFADMSQDDIDAIIAYIQTLTE"
     gene            2376..4445
                     /gene="ctaD"
                     /locus_tag="VFV93_03050"
     CDS             2376..4445
                     /gene="ctaD"
                     /locus_tag="VFV93_03050"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX5164350.1"
                     /translation="MATRVEVAPGRTTVVDRPAIAEPSGLWSWITTVDHKRIGIFYFA
                     LGAFYFLVGGIEAMMIRVQLASPNNDFVAADTYNQIFTMHGTTMIFLALMPLSSGFFN
                     FLVPLMIGARDVAFPRLNALSWWILLFGSLLLNTGWLIGQAPNAGWFSYANLTSSAYS
                     PGHNVDFWVLGIQVLGISSVISAINFLVTIINFRAPGMSMMRLPVFVWMTAVTAALII
                     LAFPALTIGITLLTMDRFYSTNFFVPEAGGSPLLWQHLFWVFGHPEVYILILPAFGIV
                     SEILPTFSRKPLFGYSAVVFSGIAIGFLGFGVWAHHMFTVGMGPLPTAVFSITTMLIA
                     LPTGVKIFNWIGTMFGGHLNFKTPLYFVLGFISMFIIGGLSGVMHASPPVDSQHQDSY
                     FVVAHFHYVLFGGSLFGLVAGAYYWFPKLAGKMLSETLGKIHFWLMFIGFNLTFFPMH
                     WVGLQGMPRRIYTYDDGYGWAIWNQIETFGALLTALSFIVFLINILISLTTGDDAPDD
                     PWDAPTLEWGIPSPPPVYNFATIPTVTSRIPLWSQKYPEIYGNDDHGLPEHVHMMDAD
                     AESEAHEQAAPLAQPDVHAHDAHDGHDSIHLPNPSYWPLVCGLGITLALGGFLGTTVS
                     LRNFIEDDMGINLWVFGVIPENLMTFVGLVMLVVSVYAWALEPAVGPGEVHDDDPSYL
                     AAGAVGD"
     gene            4601..5197
                     /locus_tag="VFV93_03055"
     CDS             4601..5197
                     /locus_tag="VFV93_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX5164351.1"
                     /translation="MAADHGVIEHPTSTGLDSRKVGMWVFLASDCMFFGSLISAYMVS
                     KNRSVVGPYPREVINVPFTSVSSFVLLMSSLAMVLALAGIQRGNIRQFRIWTLTTGLL
                     GLIFLGGQIYEFYEFNQEGLTLSRNLFGSSFFTLTGFHGTHVAIGVVWLFSMFLASFK
                     RGMVTQAKSLNVEIAGLYWHFVDIVWIVIFTLVYLLPY"
     gene            5279..5584
                     /locus_tag="VFV93_03060"
     CDS             5279..5584
                     /locus_tag="VFV93_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX5164352.1"
                     /translation="MATPSNEHAEAHAHPPAMQYVLIAAILSIITAVEVATYYIDSLE
                     AILVEILIVLSIMKFSIVVGYYMHLKFDNKLFTWVFGLGLVAGGSVTVALIALFDRF"
     gene            5673..>6011
                     /locus_tag="VFV93_03065"
     CDS             5673..>6011
                     /locus_tag="VFV93_03065"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5164353.1"
                     /translation="MLPSILPLLQANANISIWSWSPDPAVAFGVTAAAWYYYRRAKGR
                     PEDGIEPISRARQISFGLGLALIVVALMSPIGVLADDYLLSVHMIQHVTLTIAVPVLI
                     LLGIPEWMYTP"
BASE COUNT         1012 a         1957 c         1802 g         1240 t
ORIGIN      
        1 cgtccagccg atcccaatgc gccaattgac ctattgtggc cgctgaacgc ggccgaattg
       61 tttgggcgaa tcaccgattc gcccctacat tgtgattgcc gacgggtgaa caacgatgcg
      121 cttctcgctg cgtctcaaca acgatcttcc tataaaggag tatgtgcggc tggcgcaggc
      181 ggccgaggag gccgggttcg accagttctg ggtcagtcat gacctgtttt tacgctcgtc
      241 gccggcgatc ctgacggcag tggcgctggc gaccgagcgg atcgagcttg gcacctgcat
      301 tctgaaccca tacacgctgc atcccgccga actggcgatg gtggcagcga cgctggatga
      361 ggtcagcgag gggcgtttca acctcgggct gggcgccggc gcggccgcgt tcatgaagtg
      421 ggtccggctg ccctacgaac ggccgctggc gacgacgcga cgggccatcc agcaactgcg
      481 ggccgtgctg gatggcgagc agccgcccgg ttgggagccg gaagcgtatc tgcgcttcga
      541 ttccgggtca ggccgccgga ttccaatcta cctggcggcg ctctcgccat acatgctgcg
      601 tctggccggc gagctggccg atggtgtcct gccgctgctg tttccgcccg agcactacgc
      661 gaccgtcgag ccgctgatcc gcgaaggggc cgagcgggcc gggcgcagca tggcggagat
      721 cgacctggcc gcctgtgtct ggtgctcgat ctcggacgac cgccaggcgg ccgagaatgt
      781 cctgcgtgac aagatcgcct actacggcca tgccctctcg ccgctcatct tcgatcggct
      841 cggcctgacc gccggcgact tccgcgcgac cgaacgggcg ctgatgaccg agcgcgaccc
      901 gctcaaggcc cgcgcgctcg ttaccgaccg gatgctgcgg atcggcatcg tcggcacgcc
      961 tgccgacctc gtcgcccgcc tcgaatcgct ggtcgccctc ggcgtccgtc acctcagttt
     1021 cggtccgccg ctcggccccg acccgctcgc tgccgtccgg gccatcggtg cgcaactgcc
     1081 ggccgttcgg gattggtcga gctagcccgt gacgaccgta accaatcgcg gccatcagag
     1141 ttgtggtcga attcacaatc tatgctagac tcactctcag tgtgcgtgcg tcctgcctga
     1201 cattttcatc gagtggtttc tccggtgttg ggagaagggt gaatggggat ggaccgacgc
     1261 ttcccgggcc ggtggccggg gggcttccgt agtggacgcc tgttccggat tctggccgtc
     1321 gtaaccctgc tgttgctggc atttacattg agtgcctgta gcgtcaccag cgatcctcaa
     1381 tcgaccatcg ctcgagacgg tcgcgcgaac gaccgcatct ggtttgtcta tgacatcctc
     1441 tggatcggcg tcgccgtcgt cctcatcctc gttgagggcc tcctcatcta caccctgatc
     1501 cggttccgcc gccggccgaa gacggtccat ggccggccgg ttcccgtcca cggcaacacg
     1561 actctcgaga tcgcctggac gattatcccc gccgtcattc tggttgccat cagcatccca
     1621 acccttcaag tgctcgccga cctctcggac ccgcccgacg atcagccgca gatgcagatc
     1681 gatgtgatcg gtcaccagtt ctacttcgag ttccagtacc cggaagaggg cttgcagacc
     1741 accaacacgc tccacatccc cgaagacacg atcgttgaca tcgacatcac ttccggcgat
     1801 gttattcact cactttgggt gccgaggctg aacgggaaga ccgacgcggt tcccggtcgc
     1861 acgaacgaca tgtggatcga ggctgacaac cccggtacct accacgggca gtgcgccgag
     1921 ttctgcggca ccggccacgc gctgatgcgc ttcacggtcg aggtccatac caaggccgac
     1981 tacgacgcct gggtccagca gcagaaggat cgcgccgctg gagggggcga gcttgcccag
     2041 cgcggccagc agatcgcgca agagaacggc tgcaccggct gccactcgat cgacggctct
     2101 gcggcggtcg gcccgacctg gcaggggctc tttgggcacg aggtaacgct gtcggacggc
     2161 agcacggtga cggccgatga ggcctacatc accgagtcga tccgcgatcc gagcgcgaag
     2221 atcgtccagg ggttcccgga catcatgccg aagaccttcg ctgacatgtc gcaggacgat
     2281 atcgacgcga tcatcgccta tattcagacg ctgacggagt aagggcccaa ccgacaaacg
     2341 ggggttagtc agttgaggag cgaggggaaa tcgagatggc gacacgggta gaggtcgcgc
     2401 caggacgaac cacagttgtc gaccgaccgg cgatagccga gccgagtggg ctgtggagct
     2461 ggatcacgac ggtcgatcac aagcgaatcg gtatcttcta cttcgcgcta ggggcgttct
     2521 acttcctggt tggcggcatc gaggcgatga tgatccgcgt ccagctcgcc agcccgaaca
     2581 acgacttcgt cgcggccgat acctataatc agatcttcac catgcacggc acgacgatga
     2641 tcttcctggc actgatgccg ctttcgtccg gcttcttcaa cttccttgtc cccctcatga
     2701 tcggtgcgcg ggatgtcgcg ttcccgcgcc tgaatgcgct ctcgtggtgg atcctgctgt
     2761 tcggatcgct gctgctgaac accggctggc tgatcggtca ggcgccgaac gccggctggt
     2821 tctcctacgc caacctgacc tccagcgcct actcgccggg gcacaacgtc gacttctggg
     2881 tacttggtat tcaggtgctc ggaatctcct cggtcatctc agcgatcaac ttcctcgtca
     2941 cgatcatcaa cttccgcgcg cccggcatgt cgatgatgcg cctgccggtc ttcgtctgga
     3001 tgacggccgt gacggccgcc ctcattattc tcgccttccc ggccctgaca ataggcatca
     3061 cgctgctgac gatggaccgc ttctacagca ccaacttctt cgtgccggag gccggcggta
     3121 gtccgctgct ctggcagcac ctgttctggg tgttcggtca tcccgaggtg tatatcctga
     3181 tcctgcctgc cttcgggatc gtctccgaga ttctgccgac cttctcgcgc aagccgctct
     3241 tcggctactc cgccgtcgtc ttctccggca tcgcgatcgg cttcctcggc ttcggcgtct
     3301 gggcgcacca catgttcacg gttggcatgg gcccgctccc gacggccgtc ttctcgatca
     3361 cgacgatgct gatcgcgctc ccgaccggtg tgaagatctt caactggatc gggacgatgt
     3421 tcggcggcca cctgaacttc aagacgccgt tatatttcgt gctcggcttc atctcgatgt
     3481 tcatcatcgg cggcctctcg ggcgtcatgc acgcctcgcc gccggttgac tcgcagcatc
     3541 aggattccta cttcgtcgtc gcgcacttcc actatgtcct cttcggtggc tcgctgttcg
     3601 gcctggttgc cggcgcctac tactggttcc cgaagctggc cggcaagatg ctcagcgaga
     3661 ccctcggcaa gattcacttc tggctgatgt tcatcggctt caacctgacc ttcttcccga
     3721 tgcactgggt cggtcttcag ggtatgccgc gccgcatcta cacctacgac gatggctatg
     3781 gctgggcgat ctggaaccag atcgaaacct tcggcgcgct gctgacggcg ctctcgttca
     3841 tcgtcttcct gatcaacatc ctcatcagcc tgacgaccgg cgatgacgcg ccggacgatc
     3901 cgtgggacgc gccgacgctg gagtggggga tcccatcgcc gccgccggtc tacaactttg
     3961 cgaccatccc gactgtcacc agccgcatcc cgctctggtc ccagaagtat ccggagatct
     4021 acggtaacga cgaccacggc ctgccggagc atgttcatat gatggatgcc gacgccgaga
     4081 gcgaggccca cgagcaggca gcgccgctgg cccagccgga cgtccacgcg catgatgccc
     4141 acgacggcca cgattcgatt cacttgccga acccgtcgta ctggccactc gtttgcggcc
     4201 tcggcatcac actcgcgctg ggtggcttcc tcgggacgac ggtgagcctg cgcaacttca
     4261 tcgaagacga catgggcatc aacctctggg tcttcggggt catcccagag aacctgatga
     4321 ccttcgtcgg cctggtgatg ctggtcgtct cggtctacgc gtgggcgctc gagccggcgg
     4381 tcggcccagg cgaggtccac gacgatgacc cgtcgtacct cgcggccggc gctgttggag
     4441 actagatagc gggccgatcg gccagtcagc attcagcgtc agcatgatga ctggtagcga
     4501 gagacgatcg gcaacgatga accggaacgt gcgaacaggc tgatgacaca ctgactggcc
     4561 ggcggagtcg ccggccagcg aaccggaggg aacaacagga atggctgctg atcacggggt
     4621 gatagagcat ccaacaagta ccggactcga cagccgcaag gtggggatgt gggtctttct
     4681 cgcatctgac tgcatgttct tcgggtcgct gatctcggcc tacatggtct cgaagaaccg
     4741 cagtgtcgtt gggccatacc cgcgtgaggt cattaacgtt ccattcacct cggtttcgag
     4801 ctttgtcctg ctgatgagct cgctggcaat ggtgctcgcc cttgccggca ttcaacgtgg
     4861 caacatccgc cagttccgga tctggacact gacgactggc ctgctggggc tgatcttcct
     4921 cggtggtcag atctatgagt tctacgagtt caaccaggag gggctgacgc tctcacgcaa
     4981 cctgtttggg tcgtcgttct tcacgctgac cggattccac ggcacccacg ttgcgatcgg
     5041 tgttgtctgg ctcttctcga tgttcctggc atcattcaag cgcggcatgg ttacccaggc
     5101 gaagagtctc aacgtcgaga tcgccggtct ctactggcac ttcgtcgata tcgtctggat
     5161 cgtcatcttc acactcgtct atctgttgcc ctattaatac gagcgaccgg ggtccggatt
     5221 tcgaacacag gagctgagag ccgcacgcag ccggccgcga gcgacaagga gagaccgaat
     5281 ggcgacaccg agcaacgaac atgctgaggc gcacgctcat cccccggcga tgcagtatgt
     5341 gctgatcgcc gcaattctgt cgatcattac ggccgttgag gttgcgacct actatatcga
     5401 ctcgctcgaa gcgatccttg tcgagatcct gatcgtgctg tcgattatga agttctcgat
     5461 cgtcgtcggc tactacatgc acctcaagtt cgacaacaag ctgtttacct gggtcttcgg
     5521 cctgggcctg gtcgctggtg gctcggtgac ggtcgcgctg atcgccctct tcgaccgctt
     5581 ctagccaatc ttcgccggcg cggctaccag ccgcgccggc gttttcctgc cgcgctccac
     5641 tcgcggccgg acgtgacaca ggtggttctc tgatgctccc gtcgatcctg ccgctgctgc
     5701 aagccaacgc caacatctca atctggagct ggtcgcccga tcccgccgtt gccttcgggg
     5761 tgacggccgc ggcctggtac tactatcgcc gcgccaaagg ccgcccggag gacgggatcg
     5821 agccgatctc ccgtgcccgc cagatcagct tcgggctggg actggcgctg atcgttgtcg
     5881 cgctgatgtc cccgatcggc gtcctggccg acgattacct gctcagcgtt cacatgatcc
     5941 agcacgtcac gctgacgatc gccgtcccgg tcctgatcct gcttgggatt ccggaatgga
     6001 tgtacacgcc g
//
LOCUS       DASUGJ010000137         7659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_990687, whole genome shotgun sequence.
ACCESSION   DASUGJ010000137 DASUGJ010000000
VERSION     DASUGJ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990687"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFV93_03070"
     CDS             <1..253
                     /locus_tag="VFV93_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164354.1"
                     /translation="MLRLRVTTLLENAALEPDSARPQRCRDRLERALDRLTTDHVIGG
                     WRYEADPGNLPARHWLPTWTTMVIQIDPPGREPGIGIRE"
     gene            527..889
                     /locus_tag="VFV93_03075"
     CDS             527..889
                     /locus_tag="VFV93_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5164355.1"
                     /translation="MSHPIVHVEIPAENQEASGKFYSELFGWQYTAHPEFDYAMFQAE
                     GGPGGGFPKVDGESVTPGNVLVYVGTDDIEASLAKAESLGASTVVPKTEIPGTGWFAI
                     FTDPTGNRMALYTPAGEM"
     gene            966..1541
                     /locus_tag="VFV93_03080"
     CDS             966..1541
                     /locus_tag="VFV93_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5164356.1"
                     /translation="MTATRTVTILTPDRLTSLGQAFFAAQKTGEPWRCDAGIDDLAAT
                     ILERRERLRAVVAGAPEASFGPQPDNDQGEQVWTAGQCAEHVCAAQYAVGTPAILSLT
                     TPGGVATFAQPAPPNEVPAPPALSRAAALARLDAATPEFAQLLDRVPRDADLDITLDH
                     RFFGTINLAALLLICAWHEQGHAAQIERLSA"
     gene            complement(1582..1983)
                     /locus_tag="VFV93_03085"
     CDS             complement(1582..1983)
                     /locus_tag="VFV93_03085"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX5164357.1"
                     /translation="MAITTSDRMVMLWRGLTRRCPRCESGGLFRHWVEMVDDCPRCGL
                     IFEREEGYWLGAIYLNTFFTGAVLTLLLAVVGVLSWPHIPMTPMIAIGLPLGGALPIL
                     FYPSCKTFWLAIDLCFLHPLRPWRRRRREQD"
     gene            2408..4648
                     /locus_tag="VFV93_03090"
     CDS             2408..4648
                     /locus_tag="VFV93_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5164358.1"
                     /translation="MKLPTTSGLARTSALHPWRVVGVWVMLLALAGAMLIGVGMDLTT
                     EVEFTNNPDSKQGTTLLEDRLRGEDPVTETIVIRSQNTTVDDPAFKALVDQTKADLLA
                     LDGVVGSVTTYYDTDNAALVSADRNTTIIPVTLIGSFEDAETHTDEYVDAVDANRAEG
                     YEVYTVGDVSIADAFNTISEEDIQTAEGFGMPIALIILVVVFGALVAAGVPILLALVA
                     MAVSLGVTAVVSQAFELSFFVTNMITMIGLAVGIDYSLFIVERYREERRRGIAKIDAI
                     SIAGGTASKAVVFSGITVVLALAGMFIVPTSIFQSLGAGAVIVVIVSVFATLTLIPAM
                     IGLLGDKLDWPRRRKYDAAAIAAQQQYDHETIHKGFWGRITAMVMRRPVVAVVIALVL
                     LIGATIPYFDMRSGTTGVESLPESDVKSAFEILHEDFYVGVLAPVEIVIDGPATDIDV
                     QAGINSLVEDIEANDQFGSPTVETNEAGDLTLVSAPLVTDANSEASADAVTWLRESII
                     PATFNGSGADVYVTGDAAANEDLNAVIEDYTPIVFTFVLGLSFILLTLAFRSIIVPIK
                     AILMNLLSVGAAYGLLVLMFQKGYGEFLGFSETPAIEPWLPIFLFCVLFGLSMDYHVF
                     LLSRIREHYDATGRNRESVAVGLQSTAKIITGAALIMVAVFGGFASGQLVFMQQMGFG
                     LAAAVFIDATLVRSVLVPAAMALLGDRNWYLPRWLHWLPDLRVEGAAAHVEAAPVPVI
                     AGGGDD"
     gene            5046..7424
                     /locus_tag="VFV93_03095"
     CDS             5046..7424
                     /locus_tag="VFV93_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5164359.1"
                     /translation="MKSFFSTEGLARRSARHPWRVIITWVVILVVAGVATSFMNTTTE
                     FNLSSNPESEQGTDLIDQRFNTQDEFTETVIVRSDTLTVDDPAFKAHVDQITGDLTAM
                     PDVVSGASNYYQAADAGVPTAESLVSDDRHSTLIGVQLAGNLEDVTDHADAYLSVIKA
                     DRTDGFEVYSVGDLSSNEEFNSIVEEDLVSAEIIGLPITLIILVIVFGAVVAAGVPLA
                     LALMSIIIATGMAALISQAFELSFFVSNMISMIGLAVGVDYALFTMARYREERSHGVG
                     KDEAIGIAGSTANKAVVFSGMTVVLSLLGMFLIPSSIFRSLATGAILAVVVAVAATMT
                     LVPALMHLLGDRIDWKYTPWFLNSYRSGSIGRKIVATLSLLTLIPLIGLAIEGLKRVF
                     GRGDAKPAAEAGRFDDEVIHSGFWGRVTRIVMARPVVSVVLATSLLVAAAIPYFDLKI
                     GFSGVETLPESDVKTGFEILQRDFYVGAFTPVKIVVDGNVDDPELQAGVDGLLAQMAD
                     QPDQFGQPSVETAPSNDLTLISVPLATDANDDVSYDTITSLRDDWIPAQFGDRQGDVY
                     VTGVTASNEDFNQVIETYTPIVFAFVLGLSFILLMLAFRSIVVPIKAIILNLLSVGAA
                     YGLMVLVFQKGYLTGFLGFQHTPTIDAWIPIFLFCVLFGLSMDYHVFLLSRIREHYDA
                     TGRNRESVAVGLQSTARIITGAALIMVTVFSSFAAGRLVSLQQMGFGLAVAVFVDATI
                     IRSILVPAAMALLGNANWYLPRWLHWLPDLRVEGTVHSEPVSAPVTAAGGND"
BASE COUNT         1315 a         2524 c         2426 g         1394 t
ORIGIN      
        1 gatgctgcgg ctgcgggtca cgacgctgct tgagaacgcc gccctcgaac ccgacagcgc
       61 ccgcccccaa cgctgtcgcg accgtctcga acgggcgctc gaccgcctga cgaccgacca
      121 cgtcatcggc ggctggcgct acgaagccga ccccggcaac cttcccgccc gccactggct
      181 ccccacctgg acgacgatgg tgattcagat cgatccgccc ggtcgggaac cgggaatcgg
      241 gattcgggag tagaagtccc ctcccatcgt ccgtcgggaa catgaggcct cattcgcccc
      301 cgagtcccga ttcccggttc gcgctcctgc tacgccaccc tggcacgatt gctacgctat
      361 cgcggttgaa cacaacgacg ggattggcgc gctgacgccg aacgcgggtg gaaccagcga
      421 catcaatgcg tgcctggttg tcaacgatta ccccttgacg ctgtaggaca tctgttctat
      481 tctctgggga cgcgaaaccg tacgtacaca tggaagggta ccgtcgatga gtcacccaat
      541 cgttcacgtc gagatcccag ccgagaatca ggaggcgagc ggcaagtttt acagcgagct
      601 gttcggctgg cagtacaccg cccaccctga gtttgactac gccatgttcc aggccgaagg
      661 tggcccgggc ggcggcttcc cgaaggtgga tggcgagagc gtcacgccgg gtaacgtcct
      721 cgtctacgtc ggcaccgacg acatcgaggc atcgctggcg aaggccgagt cgctcggcgc
      781 cagcactgtc gttcccaaga cagagatccc cggcacaggc tggttcgcca tcttcaccga
      841 cccgaccggc aaccggatgg cgctctatac ccctgccggc gagatgtagt tgcccggaac
      901 ctgatgggcg ggatgcgatc catcgcatcc cgcagcttga ttcttcgtag agaaagccag
      961 cctgcatgac agcaacacgg acggtaacga tcctgactcc cgaccgcctg acgtcactcg
     1021 gtcaggcatt ttttgcggcg cagaagaccg gcgaaccctg gcggtgtgac gcgggcatcg
     1081 acgatctggc ggcgacgatc cttgagcgcc gcgaacggct acgggcggtc gtcgccggcg
     1141 cgcctgaggc cagctttggc ccgcagccag acaacgacca gggcgagcag gtctggacag
     1201 ccggtcagtg tgcggagcat gtctgcgccg ctcaatacgc tgtcggcacg ccggccattc
     1261 tctcgctgac gacgccaggc ggcgtagcga cgttcgcaca gccggccccg ccgaacgagg
     1321 ttcccgcccc gcccgcgttg tcacgcgctg ccgcgttggc ccggctcgat gcagccacgc
     1381 cggagttcgc gcagttgctg gatcgcgtgc cgcgcgatgc cgatctggac atcacactcg
     1441 atcatcgctt tttcgggacc atcaatctcg ccgcgctgct actcatctgt gcctggcacg
     1501 aacaggggca tgccgcccag atcgagcgtc tctcggcatg acatacgatg cgtgctcatc
     1561 gggtcgcgcc ggagcggcgg atcagtcctg ctcacgtcgc cggcgtcgcc aggggcggag
     1621 tggatgcaga aagcacaggt cgatcgcgag ccagaaggtt ttgcagcttg ggtagaacag
     1681 gattggcagc gcgccgccga gcggcaggcc gatggcgatc atgggcgtca tcgggatgtg
     1741 cggccagctc agaacgccga caacggccag cagtagcgtc agaacggcgc cggtgaagaa
     1801 ggtgttgaga tagatcgcgc cgagccagta gccttcctcg cgctcgaaga tcaggccgca
     1861 gcgggggcag tcatcgacca tctcgaccca gtgccggaag agaccgccgg actcgcagcg
     1921 cgggcagcgc cgcgtcaggc cacgccacag catcaccatt cggtcggatg ttgtgatcgc
     1981 cacctgtcca gttcccccga tcgcagctac cggcccaatc ctacgcccga gcgggtcgga
     2041 actctggcgg agacgcgact gtaccaatgg gcaggcgaga aacccttcat ccgcaggacg
     2101 tgcattcggc gatcgcaccc tatcctctgt atcagcgtcg gaaggcagac aacgacgtcg
     2161 gccaggtcgc ggtgtagcga cccgacgatc gcccgtgagt ggcaggacga cagaagggaa
     2221 acggcccagt gcatgctccg atcgtgatgg agtacatcgc cgaacagacg cagcgggaac
     2281 tggccaggac gcggggacag cgtcactggt catggatgtt ccgccgcgcg tcgtagtcga
     2341 tagatcacgt cccgcgtgtc gatcagtggg gatcggtgcg gagttcaacg gggaaggacg
     2401 aaccaacttg aaattgccaa cgacaagcgg cctcgcacga accagcgcgc tgcacccctg
     2461 gcgggtagtc ggcgtctggg tcatgctgct ggccctggcc ggcgcgatgc tgatcggcgt
     2521 cgggatggac ctgacgaccg aggtcgagtt caccaacaat ccggattcca agcagggcac
     2581 gacgctgctc gaagaccggc tgcgcggcga ggaccccgtc accgaaacga tcgtgatccg
     2641 gtcgcagaac acaaccgttg acgatcctgc cttcaaggcg ctcgtcgatc agacgaaagc
     2701 cgatctgctc gcgctcgacg gtgttgtcgg gtcggttacg acctactacg acaccgacaa
     2761 cgccgcgctc gtctcggccg atcgcaatac gaccatcatc ccggtcacgt tgatcgggtc
     2821 gttcgaggac gccgagacgc acaccgacga gtatgtcgac gcggtcgatg ccaatcgggc
     2881 cgagggctac gaggtctaca cggttggcga cgtctcgatc gccgatgcgt tcaacaccat
     2941 ctctgaagag gacatccaga cggccgaagg cttcgggatg ccgatcgcgc tgatcatcct
     3001 cgtcgttgtc ttcggcgcac tggttgccgc cggggtgccg atcctgctgg cgctggtcgc
     3061 gatggcggtc tcgctgggtg tcacggctgt cgtcagccag gcgttcgagc tatcgttctt
     3121 cgtgacgaac atgattacga tgatcggcct ggccgtcgga atcgactact cgctcttcat
     3181 cgtcgagcgc taccgtgagg aacgccggcg tgggatcgcc aagatcgacg cgatctcgat
     3241 cgcgggcggc accgccagca aggcggtcgt cttctccggt atcaccgtcg tcctggcgct
     3301 ggccggtatg ttcatcgttc cgaccagcat cttccagtcg ctcggcgccg gcgccgtcat
     3361 cgtcgtcatc gtctcggtct tcgcgacctt gacgctgatt ccggccatga tcggcctgct
     3421 cggcgacaag ctcgactggc cgcgccgccg caagtacgac gcagccgcga ttgccgccca
     3481 gcagcagtac gaccacgaga cgatccacaa gggcttctgg ggacggatca cggcgatggt
     3541 catgcgccgc ccggtcgtcg ccgtcgtcat cgcgctggtg ctgctgatcg gcgcgacgat
     3601 cccgtacttc gacatgcgct ccggcacgac cggcgtcgaa tcgctgccgg agtcggacgt
     3661 caagagcgcg ttcgagatcc tgcacgagga cttctacgtc ggcgtcctcg cgccggttga
     3721 gatcgtcatc gacggtccgg cgactgacat cgatgttcag gcggggatca actccctggt
     3781 cgaggacatc gaggccaacg atcagttcgg ctcgccgacg gtcgagacga acgaggctgg
     3841 tgacctgacg ctcgtctcgg ccccgctggt gacggacgcg aactccgagg cgtcggccga
     3901 cgccgtcacc tggctgcgtg agagcatcat cccggcgacc ttcaacggct ccggcgcgga
     3961 tgtctacgtg accggtgacg cggccgccaa cgaagacctg aacgcggtga tcgaggacta
     4021 cacgccaatc gtcttcacct tcgtgctcgg actgagcttc atcctgctga cgctggcctt
     4081 ccgctcgatc atcgtgccga tcaaggcgat cctgatgaac ctgctctcgg ttggcgcggc
     4141 ctacggtctg ctcgtgctga tgttccagaa gggctacggc gagttcctcg gcttctccga
     4201 gacacctgcc atcgagccgt ggctgccgat cttcctgttc tgtgtcctgt tcggcctcag
     4261 catggactac cacgtcttcc tgctgagccg gatccgcgag cattatgacg ccaccggacg
     4321 caaccgtgag tcggtcgcgg tcggcctgca gagcacggcc aagatcatca ccggcgcggc
     4381 gctgatcatg gtcgcggtct tcggcggctt cgcctcgggc cagctggtct tcatgcagca
     4441 gatgggcttc ggcctggcgg cggccgtctt catcgacgcg acgctggtcc gttcggtcct
     4501 cgtcccggct gcgatggcgc tgctcggcga ccgcaactgg tacctgccgc gctggctgca
     4561 ctggctgccc gacctgcgcg tcgaaggcgc cgccgcccac gtcgaagcgg ctccggttcc
     4621 ggtcatagcc ggcggaggcg acgactaacc gaatccgggc gggtccaggc acgcggggcc
     4681 gggaggcaac tcccggcccc gtcctcgttt ccagttgatg tgccgttttc gccgcctgct
     4741 cgctgagtag atagccgaag gatgacaacg ccggggcgga gcaactccgt ccaatggcgt
     4801 acgtgacctg caccgatgga caggtgaaat tcccggcctc tgacggaagt cagcggcgag
     4861 gtcgctacgg tagcgttggg gaggaagggc gtccgacgaa cgaggggagc gtatcgcgga
     4921 caacagctga cgcggaatcc agcacgctac aatgcggtct actgtgcaca acttgacagc
     4981 gtcggcgcag cgttgcgtcg atacccacgg tgaccgcccg gtcgaatgat cgttggagga
     5041 tcacagtgaa gtcattcttc tcaaccgaag gtttggcgcg acgaagcgcc cggcacccat
     5101 ggcgggtcat catcacctgg gtcgttatcc tcgtcgtggc tggtgttgcc accagcttca
     5161 tgaacacgac taccgagttc aacctctcca gcaaccctga atcggaacag ggcaccgacc
     5221 tgatcgatca gcggttcaat acgcaggatg agttcaccga gacggtgatc gtccggtcag
     5281 acacgttaac cgtggatgat ccggcattta aggcgcacgt cgaccagatc accggcgacc
     5341 tgacggcgat gccggatgtc gtatccggcg cctcgaacta ctaccaggca gccgatgccg
     5401 gtgtcccgac cgccgagagc ctcgtctccg acgatcggca ctcgacgctg atcggcgtcc
     5461 agctggccgg caacctggag gacgtgaccg accacgccga cgcctacctg tcggtgatca
     5521 aggccgaccg gaccgacggc ttcgaggtct acagcgttgg cgacctcagc agcaatgagg
     5581 agttcaacag catcgtcgag gaagacctcg tctcggctga gatcatcggt ctgccgatca
     5641 cgctgattat cctcgtgatc gtctttggcg ccgtcgttgc ggccggtgtg ccgctggcgc
     5701 tggccctgat gtcgatcatc atcgcgaccg gcatggcggc cctcatcagc caggcatttg
     5761 agctttcgtt cttcgtcagc aacatgatct cgatgatcgg cctggccgtc ggtgtcgact
     5821 acgcgctctt cacgatggcg cgctaccgcg aggagcgcag tcacggtgtc ggcaaggacg
     5881 aggcgatcgg gatcgccggc agcaccgcga acaaggcggt cgtcttctcc ggcatgacgg
     5941 tcgtcctctc gctgctcggt atgttcctga tcccgtcgtc gatcttccgc agcctcgcga
     6001 ccggcgcgat tctggccgtc gtcgtcgccg tcgccgcgac gatgacgctc gtcccggccc
     6061 tgatgcacct gctcggcgac cggatcgact ggaagtacac gccgtggttc ctgaacagct
     6121 accgctccgg ctcgatcggt cgcaagatcg tcgcgacgct ctcgctgctg acgctgatcc
     6181 cgctgatcgg gcttgccatc gagggcttga agcgggtctt cgggcggggc gacgccaagc
     6241 ccgccgccga ggccggacgg ttcgacgatg aggtgatcca cagtggcttc tggggtcgtg
     6301 tcacccgcat cgtcatggca cgtccggtcg tctcggtcgt gctggcgacg agcctgctgg
     6361 tcgcggctgc gattccgtac ttcgacctga agatcggctt ctccggcgtc gagacgctgc
     6421 cggagagcga cgtcaagacc ggtttcgaga ttctccagcg tgacttctac gtgggagcct
     6481 tcacgccagt taagatcgtc gtcgatggca atgtcgatga tcccgagctc caggccggag
     6541 tcgatggcct gctggcgcag atggcggacc agccggatca gttcggccag ccatcggtcg
     6601 agacggcgcc atcgaatgac ctgacgctga tctcggtgcc gctggcgacc gacgccaacg
     6661 acgatgtctc ctacgatacg atcacgagcc tgcgcgacga ctggattcca gcccagttcg
     6721 gcgaccgtca gggcgatgtc tacgtgaccg gtgtgacggc ctccaacgag gacttcaacc
     6781 aggtcatcga gacctacacg ccaatcgtct tcgccttcgt cctcggcctg agcttcatcc
     6841 tgctgatgct ggccttccgc tcgatcgtcg tgccgatcaa ggcgatcatc ctcaacctgc
     6901 tctcggtcgg cgcggcctac ggcctgatgg tgctggtctt ccagaagggc tatctgaccg
     6961 gattcctcgg cttccagcac acgccgacga tcgacgcctg gattccgatc ttcctgttct
     7021 gcgtcctgtt cggcctgagc atggactacc acgtcttcct gctgagccgg atccgggagc
     7081 actatgacgc caccggccgc aaccgcgagt ccgtcgcggt cggtctccag agcacggccc
     7141 gcatcatcac cggcgcggcg ctgatcatgg tgaccgtctt ctccagcttc gcggccggcc
     7201 ggctcgtctc gctccagcag atgggctttg gcctggcggt ggccgtcttc gttgatgcga
     7261 cgatcatccg ctcgatcctc gtcccggccg cgatggcgct gctcggcaat gccaactggt
     7321 acctgccgcg ctggctccac tggttgcccg acctgcgcgt cgagggtacc gtccacagcg
     7381 agcccgtgtc cgctccggtc acggccgccg gtgggaacga ctaggcgcga cctcagcgac
     7441 ggctcgacgg agcgggaagt gagcttcccg ctccgtcgca cgtttcaagc acacggagga
     7501 tgtcacgacg aactatgacg aggcgatcag cgcggcacga acggcgcttt ccggtcggac
     7561 cgtggcgctg gacgaggttg ttgtcgacta tcccggcgcg gcgctgatca tggtgaccgt
     7621 cttctccagc ttcgcggccg gccggctcgt ctcgctcca
//
LOCUS       DASUGJ010000138         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_852374, whole genome shotgun sequence.
ACCESSION   DASUGJ010000138 DASUGJ010000000
VERSION     DASUGJ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852374"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1104)
                     /locus_tag="VFV93_03100"
     CDS             complement(133..1104)
                     /locus_tag="VFV93_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEX5164360.1"
                     /translation="MATNGKIGYLAGSANARVGFAAGIEVLRQGGTAMDAAIAAVRCV
                     EDNLDDQGVGTGGVPNVLGEVELDATIMDGRTLATGAVGAIKGYPNPIEIARKVMEET
                     PHVMLVGEGAELFAKTHGFKTAELMTPESKELWQARIVGDVETSSNMYEDQYSTYMTT
                     VRNWKELLHDRIFGTTNVMAKDMDGNIACAVSTSGWGFKWPGRLGDSPVIGAGNYADN
                     RYGAAACTGRGEMAIRACSAHSVVTYMRFGMSLEEALRTAMEDLSHLVDPYAERMNVM
                     NIVAMDKDGNAAAASSAEGAAFVYQTTEMDAYEEKPRIVVPVKTRPA"
     gene            complement(1219..>3162)
                     /locus_tag="VFV93_03105"
     CDS             complement(1219..>3162)
                     /locus_tag="VFV93_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX5164361.1"
                     /translation="ITLTEWYEIGGGESGYIAVRPDNPNVILAGAQAGTFTRYDHERK
                     QELDIAVWPESNRGHGSEDYKFRTNWTSPAVFSPHDPSVVYYGSHVVHRSTSDGRQWD
                     VISPDLTRGDPETLHASGGLITKDNSGAEVYATIFTIAPSPLDANVVWAGSDDGLVHV
                     TRDGGTTWDDATPATIPDWALMSIIEASPHDAATAYLAATRYKSDDFQPYLYKTSDYG
                     KTWTKITNGIPNDDFTRVVREDPTQKGLLYAGTETGIYVSFDDGGNWQKLGGNLPVVP
                     IHDLIVKGDELLVCTHGRSFWIMDDLTIVRQLAETGTSPKGSHLFQPTDAIRSRKLTG
                     FGNTEVKGKNYLFVSGIVQTYIPIIDQYGNKRRRFLDAGQNPDDGVAIYYWLADKPGS
                     QIELAILDSAGNEVRSFKSKPENGNNDDKTPTIPAIAGLNRFVWDMRVDEATKLEAKG
                     GDQPSTAGPRVVPGEYRLRLTVGGDTHEQPLRIVPDPRHATTAEEFQQQFDLLLQIRD
                     KLSETNDAINRVRRVRDQLDSWVERAKGSKSEATLNDAASALKDTLSAAEEQLIQVKA
                     NSPKDTLHYPVMLNSKLARVGNAVASADMAPTQQARDAFADLTTRIDAQIAAINAAID
                     KDVPAFNKLVAEANLDTIVIGGA"
BASE COUNT          419 a          974 c         1134 g          635 t
ORIGIN      
        1 tccccaatcg cgtaggggcg atttcgcaaa atcgcccggc taccccaccg acaggttcgg
       61 gcgggagaac cgggcgattt tgcgaaatcg cccctacgcg attggggatt gaattgcgaa
      121 tgaacgcgcc ggttatgccg gccgcgtctt gaccgggacg acgatgcggg gcttctcctc
      181 gtaggcgtcc atctcggtcg tctggtagac gaaggcggcg ccctcggctg atgaggcggc
      241 ggcggcgttg ccgtccttgt ccatcgcgac gatgttcatg acgttcatgc gctcggcgta
      301 gggatcgacg aggtgggaga ggtcctccat cgccgtcctg agggcctctt cgagcgacat
      361 cccgaagcgc atgtaggtga cgacgctgtg ggcggagcag gcgcggattg ccatctcgcc
      421 gcggcccgtg caggcggccg cgccgtagcg gttgtcggcg tagttgccag cgccgatgac
      481 gggcgagtca ccgaggcggc ccggccactt gaagccccag ccggaggtcg agacggcgca
      541 ggcgatgttg ccgtccatgt ccttcgccat cacgttcgtc gtcccgaaga tgcggtcgtg
      601 gagcagctct ttccagttac ggacggtcgt catgtaggtg ctgtactggt cctcgtacat
      661 gttgctgctc gtctcgacat cgccgacgat gcgggcctgc cagagctcct tggactccgg
      721 cgtcatcagc tcggcggtct tgaagccgtg agtcttcgcg aacagctcgg cgccctcgcc
      781 gaccagcatg acgtggggcg tctcttccat gaccttgcgg gcgatctcga tcgggttcgg
      841 gtagcccttg atcgccccga ccgcgccggt cgccagcgtc cggccgtcca tgatcgtcgc
      901 gtccagctcg acctcgccga gcacgttcgg gacgccgccg gtgccgacgc cttgatcgtc
      961 caggttgtcc tcgacgcagc ggacggcggc aatcgccgcg tccatcgccg tcccgccctg
     1021 gcgcaggacc tcgatcccgg ccgcgaagcc gacccgggcg ttcgcgctac cggccaggta
     1081 gccgatcttg ccgttggttg ccatctgtgg tgtattcctc tccctcgtta cggtttcgcg
     1141 cctgagtcat caccggtagg ggcgagtcgg tgcctcgtcc ctaccggggg gtcccaaagt
     1201 gtttggagaa cgtcgcggct aggcgcctcc aatcacgatc gtgtcgaggt tggcctcggc
     1261 gacgagcttg ttgaaggcgg ggacgtcctt gtcgatggcc gcattgatgg cggcgatctg
     1321 ggcgtcgatc cgggttgtga ggtcggcgaa ggcgtcgcgt gcctgctggg tcggggccat
     1381 gtcggcgctg gcgacggcgt taccgacccg cgccagcttg ctgttgagca tgaccgggta
     1441 gtgcagcgtg tccttcgggc tgttggcctt gacctggatc agctgctcct cggcggccga
     1501 gagcgtgtcc ttgagcgcgc tggcggcgtc gttcagcgtc gcttcactct tcgagccctt
     1561 ggcgcgctcg acccagctat cgagctgatc gcggacgcgg cggacgcggt tgatggcgtc
     1621 gttcgtctcg gagagcttgt cgcggatctg caacagcagg tcgaactgtt gctggaactc
     1681 ctcggccgtc gttgcgtggc gcgggtcggg gacgatccgg agtggttgct cgtgggtgtc
     1741 gccgccaacc gtcagccgca gccggtactc gccgggaacg acccgcggtc cggcggttga
     1801 gggctggtcg ccgcccttgg cctccagttt ggtcgcctcg tcgacgcgca tgtcccagac
     1861 gaagcggttg agaccggcga tggccgggat cgtcggggtc ttgtcgtcgt tgttgccgtt
     1921 ctccggcttg ctcttgaacg agcgcacctc gttaccagcg ctatcgagga tcgccagctc
     1981 aatctggctg ccgggcttgt cggccagcca gtagtagatg gcgacgccgt cgtccgggtt
     2041 ctggccggcg tcgaggaagc ggcggcgctt gttgccgtac tggtcgatga tcgggatgta
     2101 ggtctggacg ataccggaca cgaagaggta gttcttgccc tttacctccg tgttgccgaa
     2161 gccggtcagc ttgcgggagc ggatcgcgtc cgtcggctgg aagaggtggc tgccctttgg
     2221 cgacgtgccg gtctcggcga gctggcggac gatcgtcagg tcgtccatga tccagaagct
     2281 gcggccgtgg gtgcagacca gcagctcgtc gcccttgacg atcaggtcgt ggattgggac
     2341 gaccggcagg ttgccgccca gcttctgcca gttgccgccg tcgtcgaagg agacgtagat
     2401 gccggtttcc gtcccagcgt agagcagccc cttctgggtc gggtcctcgc ggacgacgcg
     2461 ggtgaagtcg tcgttgggga tgccgttggt gatcttcgtc caggtcttgc cgtagtcgct
     2521 cgtcttgtag aggtagggct ggaagtcatc gctcttgtaa cgggtcgcgg ccaggtaggc
     2581 ggtcgcggcg tcgtgcggcg aagcctcgat gatgctcatc agcgcccagt cggggatcgt
     2641 cgccggcgtc gcgtcgtccc aggtcgtgcc gccgtcgcgg gtgacgtgga cgaggccgtc
     2701 gtccgacccg gcccagacga cgttggcgtc cagcggtgag ggcgcgatcg tgaagatcgt
     2761 tgcgtagacc tccgcgcccg agttgtcctt cgtgatcaga ccgccggagg cgtgcagcgt
     2821 ctcggggtcg ccacgggtca ggtcgggcga gatgacgtcc cactggcggc cgtcgctggt
     2881 cgagcggtgg acgacgtgcg agccgtagta gacgacgctc gggtcgtgcg gcgagaagac
     2941 ggccggcgag gtccagttgg tgcggaactt gtagtcctca gagccgtggc cgcggttgct
     3001 ctccggccag acggcgatgt ccagctcctg cttgcgctcg tggtcgtagc gggtgaaggt
     3061 gccggcctgg gcgccggcca ggatgacgtt cgggttgtcg ggccggacgg cgatgtagcc
     3121 ggattcgccg ccgccgatct cgtaccactc cgtcagtgtg at
//
LOCUS       DASUGJ010000139         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1964220, whole genome shotgun sequence.
ACCESSION   DASUGJ010000139 DASUGJ010000000
VERSION     DASUGJ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964220"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1393)
                     /locus_tag="VFV93_03110"
     CDS             complement(17..1393)
                     /locus_tag="VFV93_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5164362.1"
                     /translation="MADRSLLRRPLHEVAALVKRREVSPVELTSLSLEAIEQTNPTQN
                     SFRVVMADRALEAARVAEGEIANDAYRGPLHGIPIAVKDLMDVAGETTPAGSIVLADR
                     VATEDSEAVRLLREAGSVIAGKTSMPEFAFSPGSNNQHYGPVPNPWNPEHDSGGSSSG
                     SGAAVASGLVYGATGSDTGGSIRMPATLCGIVGLKPTFGRVSARGAVTLSWSLDHIGP
                     MTRTVRDTAIMLDALAGYDPGDGRTRRVPAGGYADAVEDGVAGLRVALISDDSGGQMG
                     TPAVLDALAAGVGTLEAGGAHVEQVAVPELADFAALYGALLVIEAAAYYEPFLRERPD
                     DLGEFARDRLLLAYAYSPAFFVQGSQARAALRRRIEERLTGYDLVAVPGMPHEAPPLG
                     IVQQNTRYTGPFNALGWPAIVVPVELGENNLPVAIQIAARPWQEPLVLRAGRVVERDG
                     PWQGRLPG"
     gene            complement(1386..1616)
                     /locus_tag="VFV93_03115"
     CDS             complement(1386..1616)
                     /locus_tag="VFV93_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164363.1"
                     /translation="MSADEELIRRWRANAAAAGVRLTDDDITRIEGRGAVARVRTVEA
                     IVERTNAREVVPDYLDLLSTESKAQTGERANG"
     gene            complement(1775..>2089)
                     /locus_tag="VFV93_03120"
     CDS             complement(1775..>2089)
                     /locus_tag="VFV93_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5164364.1"
                     /translation="SWNKPAAGDWTAEQHDERIAGCDVMLPWVGQADFVPLDVRAYSE
                     WSGTQPNPANQREGHIPGAVHIEWREFVNWDNNARLKPAGEIRAMLEAAGVTRDKRVV
                     PY"
BASE COUNT          321 a          750 c          687 g          331 t
ORIGIN      
        1 ctgctgcgca ggcttactag ccgggcagcc gtccctgcca ggggccgtcc ctttcgacga
       61 cccgaccggc gcggaggacg agcggctcct gccagggccg ggcggcgatc tgaatcgcga
      121 ccggcaggtt gttttcgccg agttcgaccg gcacgacgat cgccggccag ccgagcgcgt
      181 tgaatgggcc ggtatagcgc gtgttctgct ggacgatgcc gagcggcggc gcctcgtgcg
      241 gcatgcctgg aacggcgacc agatcgtagc cggtcagccg ttcctcgatc cggcgacgca
      301 aggccgcccg ggcctgagag ccctgcacga agaaggccgg agagtaggcg taggccagca
      361 gcagccggtc acgggcaaac tcgcccaggt catcaggccg ctcgcgcagg aatggctcgt
      421 agtaggcagc cgcctcgatg accagcagcg caccgtagag cgcggcgaag tcggccagct
      481 ccggcaccgc gacctgctcg acgtgcgcgc cgcccgcttc cagtgtgccg acgccggctg
      541 ccagcgcgtc cagcacggcc ggcgtcccca tctgcccgcc gctatcgtcg gagatcagcg
      601 caacacgcag gccggcgacg ccgtcctcga cggcatcagc atagccaccg gccgggacgc
      661 ggcgtgtccg gccatcgcct gggtcgtaac cggccaacgc gtccagcatg atggccgtgt
      721 cgcgaacggt tcgggtcatc gggccgatgt ggtcgagcga ccatgacaac gtcacggcgc
      781 cacgagccga gacgcgtccg aaggttggtt tgaggccgac gatgccgcac agcgtcgccg
      841 gcatccggat cgagccgccg gtgtcggagc cagtcgcgcc gtagacgagg ccgctggcga
      901 cggccgcacc ggagccgctg ctcgagcctc cgctgtcgtg ctccgggttc cacggattcg
      961 ggaccggccc gtagtgctga ttgttcgagc caggcgagaa cgcgaactcc ggcatcgagg
     1021 tcttgccggc gataacggac ccagcctcgc gcaggagacg gacagcctcg ctgtcctcgg
     1081 tcgcgacgcg gtccgccagc acgatggagc cggccggtgt cgtctcaccg gcgacatcca
     1141 tcaggtcctt gacggcgatc ggaatgccat gcagcgggcc acgataggcg tcgttggcga
     1201 tctccccctc ggcaacgcgt gccgcttcca gcgcgcgatc ggccatgacg accctgaacg
     1261 agttctgggt tggattcgtc tgctcaatcg cctcaagcga gagcgaggtc aactcgacgg
     1321 gcgaaacctc gcgtcgtttg acgagtgccg caacctcgtg cagcggccgg cgcagcagcg
     1381 atcggtcagc cattggctcg ctcccccgtc tgcgccttcg actcggtcga gagcaggtcg
     1441 aggtaatccg gcaccacctc gcgcgcgttg gtgcgctcga cgatcgcctc gaccgttcgc
     1501 acccgtgcga ccgcgccgcg tccctcgatc cgggtgatat cgtcatcggt cagccgcacg
     1561 ccggccgccg ccgcgttcgc ccgccagcgt cggatcagtt cctcgtccgc gctcatcctg
     1621 ccctccagcc tgttcccgta acctacgacg gcagcgcaac cggcgcgtct ggccggttgc
     1681 cccactcaga ccaggaacgg tcgtagttcg ccacccgctc gtaccccaac agcgtcagca
     1741 gccagactcc gtgcgccgca cggatgccgc cttgtcagta ggggaccacc cgtttgtcgc
     1801 gcgtcacgcc ggctgcttcc agcatcgccc gaatctcgcc ggccggcttg agccgggcgt
     1861 tgttgtccca gttgacgaat tcacgccact cgatatgaac ggcgcctgga atgtgaccct
     1921 cgcgctggtt ggccggattt ggctgggtcc cggaccactc gctgtaggcg cgcacgtcca
     1981 gcggcacgaa atcggcctgg cccacccacg gcagcatgac atcgcaacct gcgattcgct
     2041 cgtcgtgctg ctcggcggtc cagtcacctg ctgcgggctt gttccaact
//
LOCUS       DASUGJ010000140         9709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1547667, whole genome shotgun sequence.
ACCESSION   DASUGJ010000140 DASUGJ010000000
VERSION     DASUGJ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9709
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547667"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VFV93_03125"
     CDS             <1..1364
                     /locus_tag="VFV93_03125"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEX5164365.1"
                     /translation="AGDAIPLEDQIGQWRNYLRRRQAIHAVDVAELEDHLREQVAVLG
                     EAGLDSDEAFLVAVKRMGNLDALSREFAREHSERLWKQLVVAPAESGETRTWVRTDAV
                     VAFCLAIVAAILIKIPALFGMQIDEDAGFYARNASLFVLPLLTGYFVWKRQRSTSTVR
                     WLIGAFVVAGIFANVYPFETEGSTEILTVLHLPIALWLVVGIAYAGGRWSQVSGRMDF
                     IRFSGELFVYYTLIALGGGVLTGFTAMIFQAIGIDVERFFGSWLLPCGAVGAVLVASW
                     LVEAKQSVIENMAPVLTRLFTPLFAAMLVTFLGTLLWTGSGVDIDRDALIAFDLLLIV
                     VLGLLLYSISARDPEAPPDLFDAMQVVLVVSALLADAFALWAIAARITEFGFSANRVA
                     GLGVNAILLINLAWSTVLYVRFLRGRGPFATLERWQTSYLPIYAAWAAIVVIAFPPLF
                     GFD"
     gene            complement(1452..2315)
                     /locus_tag="VFV93_03130"
     CDS             complement(1452..2315)
                     /locus_tag="VFV93_03130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5164366.1"
                     /translation="MVRLARFTLSVAMIAMMVVALVPTTSAAPLPGIYLALGDSLLAP
                     DGFGASAPDKAAFALFFDYLQQHGAADQVVNLARGGEWSGGFSGQLAHGDRIDPASQL
                     AQALDVIDDRATDVTVISLSLGGNDVGPDSCFPDHTTAQCRSDQAANVAAFAWRLPAI
                     LQELRPALARDPGQERVLVVAPFNPFGGMGDDFSWFFDTGGWESWVDAGMAGSDGAID
                     CATPKPTYAVDDTIACVAPRHGARVVDLWTLFDDQAALYTGMDEAGDTHPNDIGHSLF
                     AAAMIDAYNAK"
     gene            complement(2352..2561)
                     /locus_tag="VFV93_03135"
     CDS             complement(2352..2561)
                     /locus_tag="VFV93_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164367.1"
                     /translation="MSAQRTLQTLVISLVLALSLALPIVEAAGMEPLQAPGFNACGYR
                     QTECVPKSSFAARTSDPHRPHFLEH"
     gene            complement(2650..3810)
                     /locus_tag="VFV93_03140"
     CDS             complement(2650..3810)
                     /locus_tag="VFV93_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164368.1"
                     /translation="MQRRFFLLAACLTLILAGCGGSGKADERDTGSSSMPTAETVQPT
                     VIRIATSADTAADPSPTATTGPSPTATRLPSATATPEEPLLPVTTTIPVGSVPASMAW
                     GAGAIWVGNGEDGTISRIDPATNTVVATIEMHANQWTLDQALNTCCPNLSLAGNDQQI
                     WAGVHRGTPATITDTVEQIDPATNQVVRTIDLPSQPEFMLLDGDTLWITSGHSDQALR
                     VDIPSGTVVATFEIKTAGAMAVADGAAWISGGFPDGSGGALYRIDPQTNTIAATIDLP
                     LGGWPFALNDELWLVEYYGNGIHRIDMATNTIAESTHVSGVLGGFPGAGSIWVNMLKD
                     ANFPGIMARIDPDTMEITARYDAGGYAGWMLFAEDAVWITELQDGLVQRIDP"
     gene            complement(3855..5273)
                     /locus_tag="VFV93_03145"
     CDS             complement(3855..5273)
                     /locus_tag="VFV93_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164369.1"
                     /translation="MLKPTRGARSSQARLLDRFVTTLRRDRRAARPPAELDQDLARMA
                     AYVELEIEPRLPDVRDAFAGTLWRRISATPETTVDLSWNGPAVDPTRLRRPVATENDE
                     SSGSRDAPSVPHPPEPFIVFQRRRWASEVLRAAAALLAFLVVGGLLTLLLRGEQERSA
                     GLGPEATGVATSTITSTPTPPSVSIPVGERPVGIAAGAGAVWITDTVDGLLRRIDPAS
                     NQVVATIEIQPPADPNDLRVGAGMLVAVGGDRVWVTRYDTTNAVTPRVALLQIDPATN
                     EIVSIAPLDGAPLGLAADADHAWIAANQRLYQFDGDGVEPVTTIPPVDGVCACGAGFS
                     VGWDGTSNGELVHIDPATGQTIATIDIGIDAVSIVNSPDAVWVLSADGGQIVRIDPAG
                     NQVVAHVDIGAPVLAIAWDQGSLWALETSGLIAQFDPGTSQVVRQLDFGMQPSTSAQL
                     AAGDGSLWVNDTEQHIIIRVNP"
     gene            complement(5267..5950)
                     /locus_tag="VFV93_03150"
     CDS             complement(5267..5950)
                     /locus_tag="VFV93_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5164370.1"
                     /translation="MVWQRFRSQHGTEATAVVPVSAVSNDGAETLDDAWLVAAALADR
                     NAFRPLYERHLPQVYHFCYLRLGSREAAEDATSEIFIKAINGLRGYHGGAFAGWLFRI
                     AQHAVVDIQRAQRRRPIVSLGVAIEDELMDPTPMPEDRAISQSELDIFRDALGRLPHD
                     QRTLLELQLAGLSTQQIAEAMGRSAGAVRMLRMRAFQQLRSLLEGSGLQPGASFQYQV
                     EPPAGGNRC"
     gene            complement(6077..7303)
                     /locus_tag="VFV93_03155"
     CDS             complement(6077..7303)
                     /locus_tag="VFV93_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5164371.1"
                     /translation="MGISSSDVLIADVVVVGGGVAGSSLAAVLASAGLGVVLVEREAS
                     FRDRVRGESIHPWGVTQLEALGLGDVLRAAGAQELPLSRFYSQRVSGDASRWDSIEPG
                     LPSELAIYHPALQMALIEHAGRCGVRLLRPARVTGFMRGREVRLTVAAQQRELEVRAR
                     LVIGADGRQSAARRWVGAEAIYEPVHHLVGGCLLDGAELADDTFHFGVFEGGNVLLFP
                     QGNGRARLYLLAKELVVGPLRGHERVADYIALCATAFPEGALADARPAGPLAFFPNAD
                     VWSDRLYDDRVALIGDAAGANDPSGGHGLSLCFRDVRDVRDQLLDTTDWDAAMRAYAA
                     RRSAYYAVLRLYMQWMVPLYIDVGPEADARRERRERARQSDPTLGGFALLPAYGPDGL
                     TADEAARRHVLGEDLD"
     gene            complement(7432..8772)
                     /locus_tag="VFV93_03160"
     CDS             complement(7432..8772)
                     /locus_tag="VFV93_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX5164372.1"
                     /translation="MRNVSRDHLVAAFAELIAEDQIQTAVELLVAAEPDEQLLAITES
                     SPDLLHSLFQALDVEDRLEVTRRLSDAARDRLLEILTETAEPEDAVTVTTPSDEVYLA
                     AEDVAEMFPSVLDDGVDAAVVESAEFELLAGRRDFAIVEGRLRESAPSSSSITIYTDP
                     SGPAQRQLLEALAIDEHTLASALDPDEIARLEHDPDDALTVIVWKRPWRETVIHPELL
                     GLTSIGLFFQPDHLTIVTAGETALLRAGDRASSVQDVLLRMMAATVNEFLVELKAVKR
                     TSREIQDELSRSIENREFLRMFQLSEGLVYHINAIDANGGVLRKLRALAPRIGFDEPN
                     LELLEDILIDNNQCSRQGQVFSTVLAGMLDARGNLINNNMNVLLKNLTIVNVIFLPLT
                     VITGIGGMSEYGVMLDDHGIGWGIGYAVFTVALLGFGVLLWMSVRAYINRTWAR"
     gene            9320..>9709
                     /locus_tag="VFV93_03165"
     CDS             9320..>9709
                     /locus_tag="VFV93_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX5164373.1"
                     /translation="MGRYVLRFDEIDQTRVAVVGGKGANLGELARIEGIRVPAGFCVT
                     TDAFARIVAEAPSIDQQLERLAQLEPGDREGIRALSAEIRQTIEAIAIPEDLVAAITG
                     QLDDHAAYAVRARATAEDLPSASYAGQQ"
BASE COUNT         1646 a         3230 c         3189 g         1644 t
ORIGIN      
        1 ccgccggtga cgccatcccg ctcgaagatc agattggcca gtggcgcaac tacctccgcc
       61 gccgccaggc gatccacgcc gtcgatgtcg cggagctgga agaccacctg cgcgagcagg
      121 tcgccgtcct cggcgaggcc gggctcgaca gcgacgaagc gttcctggtg gccgtcaagc
      181 ggatgggcaa cctcgacgcc ctctcgcgcg agttcgcccg cgagcactcg gagcgtctct
      241 ggaagcagct cgtcgtcgcc ccggccgaat cgggcgagac ccgcacctgg gtccgaaccg
      301 atgccgtcgt cgccttctgc ctggcgatcg ttgcggccat cctcatcaag atcccggccc
      361 tgttcgggat gcagattgac gaggacgccg gcttctatgc ccgcaacgcc agcctgttcg
      421 tcctgccgct gctgaccggc tacttcgtct ggaagcggca gcgcagcacc agcaccgtcc
      481 gctggctgat cggcgcgttc gtcgtggccg gcatcttcgc caacgtctat ccgttcgaga
      541 cggaaggctc gaccgagatc ctgaccgtgc tgcacctgcc gatcgcgctc tggctggtgg
      601 tcggcatcgc ctacgccggc ggacgctgga gccaggtcag cggccgaatg gacttcattc
      661 gcttctccgg cgagctgttc gtctactaca cactgatcgc actcggcggc ggcgtcctga
      721 ccggcttcac ggcgatgatc ttccaggcga tcgggatcga cgtcgagcgc ttcttcgggt
      781 cgtggctgct gccctgcggg gcagtcgggg ccgtcctggt cgcctcctgg ctggtcgagg
      841 ccaagcagag cgtgatcgag aacatggcgc cggtcctgac gcgcctcttc acgccgttgt
      901 ttgccgccat gctggtcacc ttcctcggta cgctgctctg gaccggcagc ggcgtcgaca
      961 tcgatcggga cgccttgatc gccttcgacc tgctgctgat cgtcgtcctc ggtctgctgc
     1021 tctattccat ctccgcccgc gaccccgagg cgccgcccga cctcttcgac gccatgcagg
     1081 tcgtcctggt cgtcagtgcg ttgctggccg acgcattcgc gctctgggcg atcgccgccc
     1141 gcatcaccga gttcggcttc agcgccaacc gcgtcgctgg cctcggcgtg aacgcgatcc
     1201 tgctcatcaa cctggcctgg tcaactgtcc tctacgtccg cttcctgcgc ggacgcggtc
     1261 cgttcgcgac tctcgaacgc tggcagacaa gctacctgcc catctacgcc gcctgggccg
     1321 ccatcgtcgt catcgccttc ccaccgctgt ttgggttcga ctgaccggcc actcgtgctc
     1381 gtttgcgtgc gtcgacagcc gacccaaagc cacctgttga gccatggctt tggatcggct
     1441 atcggcttcg gctacttcgc gttgtaggcg tcgatcatcg ctgcggcgaa cagcgagtgc
     1501 ccgatatcgt tcgggtgcgt gtcgccggcc tcgtccatgc cggtgtacag tgctgcctgg
     1561 tcgtcgaaca gcgtccagag gtcgacaaca cgcgcgccgt ggcgcggagc gacgcacgcg
     1621 attgtgtcat cgaccgcata cgtgggcttg ggggtcgcgc aatcgatcgc gccatcgctg
     1681 ccggccatgc ctgcgtcgac ccacgactcc caaccaccgg tatcgaagaa ccagctgaag
     1741 tcatcaccca ttccgccaaa cgggttgaac ggggcgacga ccaggacacg ctcctggccg
     1801 ggatcgcgag ccagcgccgg cctgagctcc tgcaggatcg ccggcagacg ccaggcgaat
     1861 gctgcgacgt tggcggcttg atcggaccga cattgcgccg ttgtatggtc cggaaagcac
     1921 gagtcgggtc cgacgtcatt gccgccaagc gagaggctga tgaccgtcac gtcggtcgcg
     1981 cggtcgtcga tgacgtcaag tgcctgcgcg agctggctcg ccggatcgat gcgatcgccg
     2041 tgcgccagtt gtccactgaa acctccgctc cactcgccgc cccgcgcgag gttgacgacc
     2101 tggtcggccg cgccatgctg ctgcaggtag tcgaagaaga gggcgaaggc cgccttgtcc
     2161 ggggcactgg cgccgaagcc atccggcgca aggagcgagt cgccgagcgc gagatagatc
     2221 ccaggcagcg gcgcggcgct cgtggtgggg accagtgcaa cgaccatcat cgcgatcatc
     2281 gcgaccgaaa gggtgaaccg agcgagacga accatggtat tccgatcctt tcgactcagg
     2341 agcgctgacg atcaatgctc caggaagtgc ggccggtgtg ggtcgctcgt acgagcggcg
     2401 aacgacgact tcggtacgca ttcggtctga cggtagccgc atgcgttgaa gcccggtgcc
     2461 tgtaagggct ccattccagc ggcttcgaca ataggcagcg ccagcgacag agcgaggacg
     2521 agcgagatga ccagggtttg cagggtgcgt tgagctgaca ccgcggttct ccttcgcagt
     2581 gaattcgttc gatgcgtcgt cggttgtcag gcgatcgttg tcggcgctct cctgggcgag
     2641 cgcgttggtt cagggatcga tgcgctggac caagccgtcc tgcaactccg tgatccagac
     2701 ggcgtcctcg gcgaagagca tccagcccgc atagcctcca gcgtcatagc gtgccgtgat
     2761 ctccatcgtg tccgggtcga tccgggccat gatgcccgga aagttggcgt ccttcagcat
     2821 gttgacccag atactccccg caccggggaa gcctccgagg acaccgctga cgtgggtgga
     2881 ttcggcgatc gtgttggttg ccatgtcgat ccgatggatc ccgttgccgt agtactcgac
     2941 gagccatagc tcgtcgttga gggcgaaggg ccagccgccg agcggcagat cgatcgtcgc
     3001 ggcgatcgtg ttcgtctgcg gatcgatgcg atagagggct ccgccggatc catcggggaa
     3061 cccgccgctg atccaggcag cgccatccgc gactgccatc gcgcctgccg tcttgatctc
     3121 gaaagtcgcc acgacggtgc cgcttgggat atcgacccgc agcgcctggt cgctgtggcc
     3181 ggaggtgatc cagagcgtgt cgccgtcgag cagcatgaac tccggttggg acggcaggtc
     3241 gatcgtccgg acgacctgat tggtagccgg gtcgatctgc tcgacggtgt cggtgatggt
     3301 cgcgggcgtt ccgcggtgga cgccggccca gatctgctgg tcgttaccgg ccaggctgag
     3361 attcgggcag caggtgttga gtgcctggtc gagcgtccat tggttggcgt gcatctcgat
     3421 cgtcgcgacg acggtgttgg tggccgggtc gatccgcgag atcgtgccgt cctcgccgtt
     3481 gccgacccag attgctcccg ccccccaggc catcgaggcg ggaacgctgc cgaccgggat
     3541 cgtggtggtg accggcaaga gtggctcttc tggcgtcgcg gtcgcgctgg gcagccgggt
     3601 cgccgtcggc gatggaccag tggttgccgt cggcgatgga tccgccgccg tgtccgcgct
     3661 cgtcgcgatc ctgatgacgg ttggctggac cgtctccgct gttggcatgg acgatgaccc
     3721 ggtgtcacgc tcatcggctt tgccgctgcc gccacacccg gcgagtatca gcgtgaggca
     3781 tgcggccagg aggaagaatc gacgctgcat cgtgtgaccc ccttcgtact ccgcgacact
     3841 gaacgactgc accgctacgg gttgacccgg atgatgatgt gctgctcggt atcgttgacc
     3901 cagagtgacc catctccggc ggcgagttgg gcggaggtgc tcggctgcat cccgaaatcg
     3961 agctgccgga cgacctggct ggtccccggg tcgaactgcg cgatcaggcc gctggtctcg
     4021 agcgcccaga ggctgccctg gtcccaggcg atcgccaaca ccggtgcacc gatgtcgaca
     4081 tgcgccacga cctgattacc tgccggatcg atccggacga tctgcccgcc gtctgcgctc
     4141 aacacccaga ccgcgtccgg gctgttgacg atcgacacag cgtcgatgcc gatgtcgatc
     4201 gtcgcgatcg tctgccccgt cgccggatcg atgtggacga gttcgccgtt gctcgtcccg
     4261 tcccatccga cgctgaagcc ggcgccacag gcacaaactc cgtcgacggg tggaatcgtg
     4321 gtaaccggtt caacgccgtc gccgtcgaac tgatagagac gctggttggc ggcgatccag
     4381 gcatggtccg catcagcggc cagacctagc ggagcgccgt ccagcggcgc aatagagacg
     4441 atctcattgg tggccgggtc gatctgcagg agcgcgaccc gtggtgtgac ggcgttggtc
     4501 gtgtcatatc tcgtcaccca gactcggtcg ccgccgacgg cgaccagcat cccggcgcca
     4561 acccgaaggt cgttcggatc agccggtggt tgaatctcga tcgtcgcgac gacctgattg
     4621 ctggccgggt cgatccggcg caggagaccg tcgaccgtgt cggtgatcca gaccgcgccg
     4681 gcgccggccg cgatcccgac cggacgttca ccaaccggga tcgagacgct gggcggtgtc
     4741 ggtgtactgg tgatcgtcga cgtcgcgaca cccgtcgcct ccggccccaa cccggcgcta
     4801 cgctcctgct cgccgcgtag cagcaacgtc aagagtccgc ctacgacgag gaaagccagc
     4861 aacgccgccg ccgcccgcaa cacctcgctg gcccagcgtc gccgctgaaa aacgatgaac
     4921 ggttcggggg ggtgaggaac ggacggggca tcccgcgaac cggacgactc gtcgttctcg
     4981 gtcgcaactg gacgccgcaa ccgggtcgga tcgaccgccg gtccgttcca ggagagatcg
     5041 accgttgtct caggcgtcgc gctgatacgg cgccagagcg tcccggcgaa agcgtcacgc
     5101 acgtcgggca gccgtggctc gatctcaagc tcgacgtagg ccgccatccg cgccagatcc
     5161 tggtcgagtt cagccggcgg acgggcagcg cgccgatccc gccgcagggt cgtgacaaac
     5221 cggtcgagca ggcgtgcctg gctggagcgc gcaccgcggg tgggtttcag catcggttcc
     5281 ctcccgccgg tggctcaacc tggtactgga agcttgcgcc cggctgcaac ccggagcctt
     5341 caagcaggga gcgtaattgc tggaacgcgc gcatccgcaa catccggacg gctccggcac
     5401 ttcggcccat tgcctcggcg atctgctgag tgctcagccc ggccagctgg agttccagca
     5461 gggtacgctg atcgtgtggc aacctgccaa gggcatcacg gaagatgtcc agttcgctct
     5521 ggctgatggc gcgatcctca ggcatgggtg tggggtccat cagttcatcc tcgatcgcaa
     5581 cgccaaggga aacaatcggg cggcgtcgtt gcgcgcgctg gatgtcgaca accgcgtgct
     5641 gagcgatacg aaacagccag ccggcgaacg ctccaccgtg atagccgcgt aggccgttga
     5701 tggccttgat gaagatctcg ctggtcgcgt cctcagccgc ctcacggcta ccgagacgca
     5761 ggtaacagaa gtggtagacc tgcgggagat gccgctcgta caacggacga aaggcgttgc
     5821 gatccgcaag cgccgccgcg accagccagg catcatcgag tgtctccgcg ccgtcattcg
     5881 acacggcgga caccggcacg acggcggtcg cctcagtccc atgctgactt cggaagcgct
     5941 gccagaccat gcgcgccacg ctctcatctg cgtcgtcagc gtgtgtctct atctacactg
     6001 acgacggcaa tctgaaaagt gtcacggtcg gcacgtccga aatcgccagt ctgccgcaac
     6061 gacagcggta ggttgatcag tcgagatcct cgccaagcac gtggcgccgg gccgcctcgt
     6121 cagcggtcag tccgtccggg ccgtaggccg gcagcagcgc gaagccgccg agcgtcgggt
     6181 cggactgcct ggcccgctcg cggcgctcgc ggcgggcgtc cgcctccgga ccgacatcga
     6241 tgtagagcgg caccatccat tgcatgtaga ggcgcaggac ggcgtagtac gccgaacgcc
     6301 gggcggcata ggcccgcatc gccgcgtccc agtcggtggt gtcgagcagt tggtcgcgca
     6361 cgtcgcgcac atcacggaag cacagcgaca acccgtggcc cccactcgga tcgttcgccc
     6421 cggcggcgtc accgatcagc gccacgcgat cgtcgtagag ccggtcggac cagacgtcgg
     6481 cattcgggaa gaacgcgagc ggcccggccg gacgggcatc ggctaaggct ccctctggaa
     6541 aggccgttgc acagagcgcg atgtagtcgg ccacgcgttc gtgcccgcgc agaggcccga
     6601 ccaccagctc cttggcgagt agatagaggc gggcgcggcc gttcccctgt ggaaacagca
     6661 gaacattgcc accctcgaac acgccgaagt ggaaggtatc gtcagccagc tccgctccgt
     6721 cgaggaggca cccgccgacg aggtggtgga ccggctcgta gatcgcctcg gcgccgaccc
     6781 agcgccgcgc cgccgattgc cgtccgtcag caccgatcac cagccgtgcc cgcacctcaa
     6841 gctcacgctg ttgcgctgcg acggttagac gcacctcccg gccgcgcatg aacccggtca
     6901 cgcgggccgg acgcagcagc cggacaccgc agcggcctgc gtgctcgatg agcgccatct
     6961 gcaacgccgg gtgatagatc gccaattcac tgggcaaccc cggctcgatg ctgtcccagc
     7021 ggctggcatc acccgacacg cgctgggaat agaagcgcga gagtggcagt tcctgtgcac
     7081 cggcggcgcg caggacatcg ccgagaccga gcgcctcaag ctgggtaacg ccccacgggt
     7141 ggatactctc gccccgcacg cggtcccgga agcttgcctc gcgctcgacg agcacaacgc
     7201 cgagtcccgc cgaggccagc accgccgcca gactggaccc ggccacacca ccgccgacaa
     7261 ccacaacatc ggcaatgagc acgtcggagg aggagatgcc cacgttgaca gtctccgttc
     7321 gtgttcgtct ctggatacgc cggctcagtg tacgatcagg agtgccatca tatgctgctt
     7381 caccagaagc gacagtactc tcaggctggc tgcgtcatcg gcgcgccctc ctcagcgggc
     7441 ccaggtacgg ttgatgtagg cacgcacgct catccagagc aggacaccaa agccgagcag
     7501 cgccacggtg aagacggcat acccgatccc ccagccgatc ccatgatcgt cgagcatgac
     7561 accatactcg gacatgccgc cgatgccggt gatgacggtg agcggcagga agatgacgtt
     7621 gacgatcgtc aggttcttga gaagcacgtt catattgttg ttgatcaggt tcccacgcgc
     7681 gtcgagcatt ccggccagga cggtcgagaa gacctgaccc tggcggctac actggttgtt
     7741 gtcgatcagg atgtcctcca gcaactccag gttcggttcg tcgaagccaa tccggggggc
     7801 gagcgcgcgc agcttgcgca acactccgcc gttcgcgtcg atggcgttga tgtggtagac
     7861 gagcccctcg ctgagctgga acatcctgag gaactcgcgg ttctcaatcg aacggctcaa
     7921 ctcgtcctgg atctcgcgtg aggtccgctt gacggccttg agctcgacga gaaactcgtt
     7981 gaccgtcgcg gccatcatcc gtagcagcac gtcctgcacc gatgaagccc ggtctccggc
     8041 gcgtagcagc gccgtctcgc cggccgtgac gatcgtcagg tggtcgggct ggaagaacag
     8101 cccgatcgag gtcagcccga gcagttcagg gtggatgaca gtctcgcgcc acggccgctt
     8161 ccatacgatg acggtcaacg cgtcatcagg atcgtgctcc agccgcgcga tctcgtcggg
     8221 gtcgagggcg gaggcgaggg tgtgctcgtc gatggcaagt gcctccagca gttgccgttg
     8281 ggccggtccg gacggatcgg tgtagatggt gatcgacgag gacgacggcg cggactcccg
     8341 caggcgtccc tcaacgatcg cgaaatcccg ccggccagcg agtagctcga actcggcgga
     8401 ctcgacgacg gcggcatcga ctccgtcgtc gagcacggac gggaacatct ccgcgacgtc
     8461 ctcggccgcg aggtagacct cgtcggatgg ggtggtgacg gtgacagcgt cctccggctc
     8521 ggcggtttca gtcaggatct ccagcaagcg atcgcgcgcc gcgtcggaca gccggcgggt
     8581 aacttccaac cggtcctcga catcgagcgc ctgaaagaga ctgtgcagca gatcggggga
     8641 gctttcggtg atcgcgagca gctgctcgtc gggttcggca gcgaccaaca gctccaccgc
     8701 cgtctgaatc tgatcctcag cgatcagttc cgcgaaggcg gccaccaggt ggtcgcggct
     8761 gacattgcgc attggaacgc ctccatctcc atctgtcgga caacatgaac cgacttccgg
     8821 aaccggcgct ccagcagcag atcatccagg atctggcaat ggctgaggtc acaaacgcat
     8881 cccccggcac cgcggggaag gaggcaaggc gtcaggcagc gaggagtctg cgcgattgcg
     8941 ttccgatcga cccgtggcgc cggcagcatt cgtccgccgg ctcggttgtc tgacggcagg
     9001 tactaccgcc caccaggaac acctccacga ggccacagac ccgcgattcg cacaaatcgc
     9061 ggtgccgtcc gcaggtacgg aacacgagcc ggtcggcatg gccgtccaac ccgtcggcgg
     9121 tggcatccta cacccactgc gggcgcaccg caagatccgg gtagcgggcg ttctagcaag
     9181 gccccctgtt tgtgagacct gcccacgggt cgtttaatca gcggacgcgt ggtgcatgac
     9241 gggcgatgac cgcttctgcg agagagaggc tggtgagacg ctgaggcatt ctcgggcgaa
     9301 cagacaggga ggggagcgga tgggccgcta cgtgttgcga ttcgatgaga tcgaccagac
     9361 ccgggtcgcg gtcgtaggcg gcaagggcgc caacctgggc gaactggcac ggatcgaggg
     9421 catccgcgta ccggccggct tctgcgtgac gaccgacgct ttcgcgcgga tcgtggccga
     9481 agcgccgtcg atcgaccagc agctggagcg gctggcgcag ctggagccgg gcgaccggga
     9541 gggaatccgc gccctcagcg ccgagatccg ccagaccatc gaagcgatcg ccatccctga
     9601 ggatctggta gcagcaatca ccggccagct cgacgatcac gccgcctacg ccgtccgagc
     9661 cagagcgaca gccgaggacc tgccgtcagc ctcctacgcc ggccagcag
//
LOCUS       DASUGJ010000141         4441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_18740, whole genome shotgun sequence.
ACCESSION   DASUGJ010000141 DASUGJ010000000
VERSION     DASUGJ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18740"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="VFV93_03170"
     CDS             <1..1855
                     /locus_tag="VFV93_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5164374.1"
                     /translation="WDYPRWSIPEKEAGRAFFFKNDGLQNQAVLYVTLAPDAEPVELL
                     DPNALSEDGTVAVTAVSASDDGRLLGYALAEAGSDWQRLRVRVVDERDDLPDLIRWCK
                     FSPIAWTRDSAGFFYSRFPEPGTVAAEDANNYNTVYYHRIGDQQAQDTLIYERPDEKE
                     LHFHPTVTHDGSYLVITAVHGTDPRARIYYREIDGDGSVIPLIEEADAKYEFIDNDGP
                     VFLFLTDLDAPRGRVIAIDTRQPAREHWREVIAEGEHPLAFVVAAGERLVVVRMRDAQ
                     HRLALHQRDGSFDREVDLPGIGSIESISGKPSDPELFVAFTSFLYPTTPFRLDLANAG
                     ATLEPLHESGIDFDTSAYETTQVFYASKDGTRVPMFLTHKRGLTLDGSNPTLLYGYGG
                     FNISLTPSFAIHRLAWLEAGGVYALANLRGGEEYGEEWHQAGMLEKKQNVFDDFIAAA
                     EWLIDNGYTSRAKLAINGGSNGGLLTGACLTQRPDLYGAVVCEVPVTDMLRFHKFTVG
                     HYWVPEYGNAETNSEHFRFMLAYSPLHNVREGVAYPPTLITSADTDDRVVPAHAKKFA
                     ATLQAAQSGDNPILIRIETRAGHGLGKPTSKIIAERADVYAFLFDILGIDA"
     gene            2390..2650
                     /locus_tag="VFV93_03175"
     CDS             2390..2650
                     /locus_tag="VFV93_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164375.1"
                     /translation="MAESMVHDLQDVAAEWDQLPEGERAAWSIDWDNEMAGVESLSRL
                     AAGGVLTADLYSRYRQLLAAIHQSTPILDRLRLSLPTSIHNP"
     gene            2668..3333
                     /locus_tag="VFV93_03180"
     CDS             2668..3333
                     /locus_tag="VFV93_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX5164376.1"
                     /translation="MTLPTFITFDCYGTLVDFDLETVTIRTLADRAAGIDLDAFLRDF
                     EAIRFQAVLGPYRPYREVLRQSLARAMEQHGLTYRDMDGDAIIAAVPTFGPFPDVPPV
                     LERLRQHCKLVIVSNTEDDLIADNVRNIGVPFDDVITAEQAGAYKPSPAIFAYVLSRL
                     GCDASDILHVAQGFEYDIMPAHDLGWKRVWINRSGRQGDAAYGPYDELPDLTGLPALL
                     GRQ"
     gene            complement(3350..4273)
                     /locus_tag="VFV93_03185"
     CDS             complement(3350..4273)
                     /locus_tag="VFV93_03185"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5164377.1"
                     /translation="MKEPFLSSEKMHDNEVMTDEALVRRLLAAQFPEWADLPIERVRS
                     AGTDNAIYRLGDELAVRLPRIDWAIEAIEKECRWLPFLAPQLPLPVPVPLAMGDPSEG
                     YPWHWSVCRWLDGENATLDRIADARQFAADLAAFVVALQRIDPVDGPRSGTPGVSRGV
                     PLATRDAGTRAAISELDGIIDTVAATAAWDAALVAPLWEAPPVWLHGDLQSGNLLVND
                     GHLSAVIDFGCLAVGDPACDMMVAWTLLTADSRAAFRAAVAVDDATWARGRGWALSFG
                     VGAFSYYKDTNPVLAGIGRHAIEQALADRDG"
BASE COUNT          809 a         1487 c         1402 g          743 t
ORIGIN      
        1 gtgggactat ccgcgctggt cgatcccgga gaaggaggcc ggccgcgcct tcttcttcaa
       61 gaacgacggc ctccagaacc aggccgtcct ctacgtcacg cttgcaccgg acgccgaacc
      121 ggtcgagttg ctcgacccga acgcgctctc cgaggacggc acggtcgcgg tgacggcggt
      181 ctccgcctcc gacgacgggc ggctgctggg ctacgcgctg gcggaggccg gcagcgactg
      241 gcagcggctg cgggtgcgcg ttgtcgacga gcgcgacgac ctgccggacc tgatccgctg
      301 gtgcaagttc tcgccaatcg cctggacccg cgacagcgcc ggcttcttct actcgcgctt
      361 ccctgagccg gggacggtcg ccgccgagga cgccaacaac tacaacacgg tctactacca
      421 ccggatcggc gaccagcagg cgcaggacac gctcatctac gagcggcccg acgagaagga
      481 gctgcacttc cacccgaccg tcacccacga cggctcctac ctggtcatca cggccgtcca
      541 cggcaccgat ccacgcgcac gcatctacta ccgcgagatc gacggcgacg gcagcgtcat
      601 cccgctgatc gaggaggccg acgccaagta cgagttcatc gacaacgacg gcccggtctt
      661 cctcttcctg accgatctcg acgccccgcg cgggcgggtg atcgcgatcg acacccgcca
      721 accggcccgc gagcactggc gcgaagtgat cgccgaaggg gaacacccgc tggcgttcgt
      781 cgtcgcggcc ggcgagcggt tggtcgtcgt ccgcatgcgc gatgcccagc accggctggc
      841 gctccaccag cgcgacggca gcttcgaccg cgaggtcgat ctgcccggca tcggctcgat
      901 cgagtcaatc tccggcaagc ccagtgaccc ggagctgttc gtcgccttca cctcgttcct
      961 ctacccgacg acacccttcc ggctcgacct ggccaatgct ggcgcgacgc tggaaccgct
     1021 gcacgagtcc ggtatcgact tcgacacgtc ggcctacgag acgacccagg tcttctacgc
     1081 ctcgaaggac ggcacccgcg tcccgatgtt cctgacccac aagcgcgggc tgacactgga
     1141 cggcagcaac ccgacgctcc tctacggtta cggcgggttc aacatctcgc tgacgccgag
     1201 cttcgccatc caccggctgg cctggctgga ggccggcggc gtctacgcgc tggccaatct
     1261 gcggggcggc gaggaatacg gcgaagagtg gcaccaggcc gggatgctgg agaagaagca
     1321 gaacgtcttc gacgacttca tcgccgccgc cgagtggctg atcgacaatg gctacaccag
     1381 tcgcgcgaag ctggcgatca acggcggcag caacggcggg ctgctgaccg gcgcctgcct
     1441 gacgcagcga ccggatctct acggggccgt cgtctgcgaa gtgccggtga ccgacatgct
     1501 gcgcttccac aagttcacgg tcggccacta ctgggtgccg gagtacggca acgccgagac
     1561 gaactccgag catttccgct tcatgctggc ctactcgccg ctccacaacg tccgcgaggg
     1621 cgttgcctac ccgccgacgc tgattaccag cgccgacacc gacgaccgcg tcgtcccggc
     1681 ccacgccaag aagttcgccg ccacgctcca ggccgcccaa tccggcgaca atccgatcct
     1741 gatccgcatc gaaacccgcg ccggccacgg cctcggcaaa cccaccagca aaatcatcgc
     1801 cgaacgcgcc gatgtctacg ccttcctgtt cgacatattg ggaatcgacg cgtgacggag
     1861 gtgtcggttc aggagctgaa agacatcgcg attcagggat ctcgcggcct gaatgctgat
     1921 gagcttcagg tgattctcga gcacatcgcg agtgtcggct tcgatccgca cgcccgtgaa
     1981 cgagtgcggg ggcgcttggc cgggacaacc atccttagag gaaccgacag gcttcctcct
     2041 gccgccgtac attacctctg gcatgtcatt ctgcgtcggg agtggccctc tggaacgacc
     2101 tgcgggacta ccttgccagt gtgcaggagg ttattcggga tccgtcgagc gctgttgtcg
     2161 catttcggta tcaaggcgca ccgcagttcg gcgtgtttcg cgaatctggc agccttcgtg
     2221 ggccgatggg gcacgagtgg gtgttgatac aataccgtgt gtcgatcgac cactgggtaa
     2281 ccgcctttca gcccgtacgt ggtctcaata tcgccaacga cccgcaatgg acggacgttc
     2341 aatggttgcg acaaccgaca cgctcgaacg tgcgatgatg agcctctcga tggctgagtc
     2401 gatggttcac gatctacagg acgttgccgc tgagtgggat caactccctg aaggcgaacg
     2461 cgcagcctgg agcatcgatt gggataacga gatggccggc gttgagagcc tgtctcgact
     2521 tgccgcgggt ggtgtgctga cagcggatct gtattcgcgg tatcgacaac tgctcgccgc
     2581 gatacatcaa tccacaccga ttctcgaccg gctccgattg tcgttaccaa cgtcgatcca
     2641 taatccataa tgaggcgggc tcggaggatg acgcttccga cgttcatcac gttcgactgc
     2701 tacggcacgc tggtcgattt cgaccttgag acggtcacga tccgaacgct ggctgatcgg
     2761 gcagcgggga tcgatctcga cgcgttcctg cgcgacttcg aggcgattcg gttccaggca
     2821 gttctcggcc cgtacaggcc ctatcgcgag gtgctgcgcc agagtctggc gcgggcgatg
     2881 gagcagcatg ggctgacgta tcgggacatg gacggggacg ccatcatcgc ggccgttccg
     2941 accttcgggc cgttcccgga cgtgccgccc gtcctcgaac gcctgcgaca gcactgcaag
     3001 ctcgtgatcg tctcgaacac cgaggacgac ctgatcgccg acaacgttcg caacatcggc
     3061 gtgccgttcg acgacgtcat taccgccgag caggcgggcg cgtacaagcc gtcgccggcg
     3121 atctttgcgt acgtgctgtc gcggctgggg tgtgacgcat ccgacattct ccatgtcgcg
     3181 caggggttcg agtacgacat catgccggcc cacgacctcg gctggaagcg ggtctggatc
     3241 aaccgcagcg ggcggcaggg tgacgctgcc tacgggccat acgacgaact accggacctg
     3301 accggattgc cggcgttact tggccgccag tagcgcaggt tgccgcgact cagccgtcgc
     3361 ggtcggccag cgcctgttcg atggcgtggc gcccgatgcc ggcaaggacc gggttggtgt
     3421 ccttgtagta cgagaaggcc cccacgccaa atgagagcgc ccagccacgg ccccgcgccc
     3481 aggtggcgtc gtcgacagca acggcggcgc ggaaggcggc acgggaatca gcggtaagca
     3541 gcgtccaggc gaccatcatg tcgcaggcag ggtcgccgac tgccaggcaa ccgaaatcga
     3601 tgacggcgct gaggtggcca tcgttgacca gcaggttgcc agattgcaga tcgccgtgca
     3661 gccagaccgg aggggcctcc caaaggggcg cgaccagcgc ggcgtcccag gcggctgttg
     3721 ccgccacggt atcgatgatc ccatcgagct ccgagatcgc ggcgcgggtt ccagcgtcgc
     3781 gggttgccag cggcacaccg cgtgagacgc ctggcgtgcc ggagcggggg ccatcgacgg
     3841 gatcgattcg ctggagggcg acgacgaacg cagccagatc agcggcaaac tgtcgtgcgt
     3901 ctgcgatgcg atccagcgtc gcgttctcgc cgtcgagcca ccgacaaacg gaccagtgcc
     3961 aggggtagcc ctcgctagga tcgcccatcg cgagcggaac cgggaccggc agcggcagct
     4021 gcggcgccag aaatggcagc caccggcact ccttctctat cgcctcgatc gcccaatcga
     4081 tccgcggcag tcgaacggct agctcgtcgc ccagccggta gatcgcgttg tcagtaccag
     4141 ccgaacgaac ccgttcgatc ggcaaatccg cccactcagg gaactgcgcc gccagcaagc
     4201 gtcgcacgag cgcttcgtcg gtcatcacct cattgtcgtg catcttttcc gaggacagga
     4261 atggctcctt catctacagc gcaactacaa atcatcaggc gtacggatcg ggcaggagcg
     4321 tcaggttcgt ctctcgggca acgacgccga aacatcgcaa acgtatccag tgggatcacg
     4381 caggctgcca gttgtcctgt agccaaccct cgacagcggt gccgttgagt tccgtatcac
     4441 c
//
LOCUS       DASUGJ010000142         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_713474, whole genome shotgun sequence.
ACCESSION   DASUGJ010000142 DASUGJ010000000
VERSION     DASUGJ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713474"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2410
                     /locus_tag="VFV93_03190"
     CDS             <1..2410
                     /locus_tag="VFV93_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5164378.1"
                     /translation="KVKIVASLTVSAIMMLLMFWPVWLLGDRPVDEMEDLYKPLFLMA
                     TPIQFWAGWQFYREAWRAARHLTVNMSTLVVMGTTAAWGYSTFVTFRTETIHQSGLMA
                     EVYFDSATIIIGLILFGRFLEARAKAQTTGAIKTLMGLAPKTARVVRDGAELDIPIEQ
                     VVGGDIVRVRPGEKIAVDGIIVDGRSSIDESMLTGESLPVEKTQGDEVIGATINKTGS
                     FTFRATKVGKDTALSQIIKLVSDAQGSKAPIQRLADQISMYFVPAVIVLALATFALWY
                     LVGPEPSFNMALQVMIAILIIACPCAMGLATPTAIMVGTGKGAENGVLIRGGEALEQA
                     HKVRAIVLDKTGTITRGKPSLTDLVPAAGFDERDLLRLAASAEQGSEHPLGEAIVVAA
                     RERGLALADVSDFDAIAGHGISAKIDGRQVLLGNRKLMAQQQIDTDAHQDAARSMADA
                     GRTPMYVAVDGQFAGIIAVADTIKPESPEAIEKLEALGLDVWMLTGDNEATAAAVARQ
                     AGIAPERVLADVLPGEKADKIRELQSRGLAVAMVGDGINDAPALAQADLGIAIGTGTD
                     VAMEASDITLMGGDLRGVVSAIALSNATVKTIKQNLFWAFGYNVILIPVAAGALYPFI
                     GELLSPALAAGAMALSSVSVVTNSLRLRGFKVPENAREIAHPPLRRRVAEASYLILLA
                     VLGLGIGAGYFGYQWWQDASAEEIVIVGSEFNFSRDTITIQEDERYRIVFENEGVVFH
                     DFIIDGVWEAHANAPAGRSGEFTIQIDEPGEYTYYCSVEGHEQAGMSGTLIVTPKGGS
                     ASAD"
BASE COUNT          431 a          834 c          788 g          382 t
ORIGIN      
        1 gaaggtcaag atcgtcgcct cgctgaccgt cagcgcgatc atgatgctgc tgatgttctg
       61 gccggtctgg ctgctgggcg accgaccggt cgatgagatg gaagatctct acaagccact
      121 cttcctgatg gcgacgccga ttcagttctg ggccggctgg cagttctatc gcgaagcatg
      181 gcgcgccgcg cgacacctga cggtcaatat gagcacgctg gtcgtgatgg gcacaaccgc
      241 cgcctggggc tacagcacct tcgtcacctt ccggaccgaa acgatccacc agtccggcct
      301 gatggccgag gtctacttcg acagcgcgac gatcatcatc ggcctgatcc tgttcggccg
      361 cttcctcgaa gcgcgcgcca aggcccagac gaccggcgcg atcaagacgc tgatgggcct
      421 cgcgccgaag acggcccgtg tcgtccgcga cggcgcggaa ctcgacatcc cgatcgagca
      481 ggttgtcggc ggcgacatcg ttcgcgtccg gcccggcgag aagatcgccg tcgacggcat
      541 catcgtcgac ggccgttcaa gcatcgacga gagcatgctg accggcgaga gcctgccggt
      601 cgagaagacg cagggcgacg aggtgatcgg cgcgaccatc aacaagaccg gctcgttcac
      661 cttccgggca accaaggtgg gcaaggacac tgccctttcc cagatcatca agctggtttc
      721 cgacgcgcag ggctcgaagg cgccgatcca gcggctggcc gatcagatct cgatgtactt
      781 cgtcccggcc gtcatcgtcc tggcgctggc gaccttcgcc ctctggtatc tggtcgggcc
      841 ggagccgtcg ttcaacatgg cgctacaggt catgatcgcc atcctgatca tcgcctgccc
      901 ctgcgcgatg gggctggcga caccgaccgc catcatggtc ggcaccggca agggcgccga
      961 gaacggcgtt ctgatccgtg gcggtgaggc acttgagcag gcccacaagg tccgcgcgat
     1021 cgtcctcgac aagaccggca cgatcacacg cggcaagccg agcctgaccg atctcgttcc
     1081 ggccgccggc ttcgatgagc gcgacttgct gcggctggcg gcatcggccg agcagggctc
     1141 cgaacatccg ctcggcgagg caatcgtcgt ggccgcccgt gagcgcgggc tggcactggc
     1201 cgacgtcagc gacttcgacg ccatcgccgg ccacggcatc agcgcgaaga tcgacggccg
     1261 ccaggtgctg ctcggcaacc gcaagctgat ggcgcagcag cagatcgaca ccgacgccca
     1321 ccaggacgcc gcccgctcga tggccgacgc cggccggacg ccgatgtacg tcgctgtcga
     1381 cggtcagttc gccggaatca ttgccgtcgc cgatacgatc aagccggaat cgccggaagc
     1441 aatcgagaaa ctggaagcac tcggcctcga cgtctggatg ctgaccggcg acaacgaggc
     1501 gaccgccgcc gccgtcgccc gccaggccgg catcgcgccg gaacgggtgc tggccgacgt
     1561 cctgcccggc gaaaaggccg acaagatccg cgagctgcaa tcacgcgggc tggctgttgc
     1621 gatggtcggc gacggcatca acgacgcccc ggcgctggcc caggccgacc tcgggatcgc
     1681 gattgggacc ggcaccgacg tcgcgatgga agccagcgac atcacgctga tgggcggcga
     1741 cctgcgcggc gtcgtcagcg cgatcgcgct ctcgaacgcg accgtcaaga cgatcaagca
     1801 gaatctgttc tgggcgttcg gctacaacgt gatcctgatc ccggtcgcgg ccggtgcgct
     1861 ctacccgttc atcggcgagc tgctctcacc ggcgctggcg gccggcgcga tggcactctc
     1921 cagcgtcagc gtcgtcacca attcgctgcg cctgcgcggc ttcaaggtgc cggagaacgc
     1981 acgcgaaatc gcccatccac ccctgcgccg ccgggtcgcc gaagcgtcgt acctgattct
     2041 gctggccgtt ctcggcctcg gcatcggcgc cggctacttc ggctaccagt ggtggcagga
     2101 cgccagcgcc gaagagatcg tcatcgtcgg ctcggagttc aacttcagcc gtgacacgat
     2161 cacgatccag gaggacgaac gctaccggat cgtgttcgag aacgagggcg ttgtcttcca
     2221 cgacttcatc atcgacggcg tctgggaggc gcacgccaac gccccggccg gccgcagcgg
     2281 cgagttcacg atccagatcg acgagccggg cgaatatacc tactactgct ccgtcgaagg
     2341 ccacgagcag gccggcatgt ccggcacgct gatcgtcacc ccgaagggcg gctccgccag
     2401 cgctgattga catggcccaa tctcccggta ggggc
//
LOCUS       DASUGJ010000143         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1547995, whole genome shotgun sequence.
ACCESSION   DASUGJ010000143 DASUGJ010000000
VERSION     DASUGJ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547995"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            538..1494
                     /locus_tag="VFV93_03195"
     CDS             538..1494
                     /locus_tag="VFV93_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5164379.1"
                     /translation="MSLQWFEGPAPHFLISSSEDATSQLSTTAPELNEPLPPWLDIAF
                     LNRLDALNASATAVVGRQAHIAHDVPISESIATFAHDIRTPLATLHASLEILADGTPP
                     DTCDLQHLAGTMQRGVTWITQLVAELARQSETSHGTGCWGTPVTSGPTEVRDWIEQAI
                     EIVQPLAGTREQAILFSCPQPSPVVEGDHVQFGQVIVNLLTNACRYGSWGDTIAVSVS
                     ERLETVTIRVSDHGMGILPDERERIFERRARGTQTGSHLVPGQGLGLYIVRGIVERHG
                     GSVTVESALGQGSTFAVHLPAARPVQPLFLRRAVVREDGAIE"
     gene            1491..2204
                     /locus_tag="VFV93_03200"
     CDS             1491..2204
                     /locus_tag="VFV93_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5164380.1"
                     /translation="MKVLLVDDDIDLLDVTSYALRREGFNVIVATDGLQAVQRWESDR
                     PDLIVCDISMPRMNGLELCQRIRRDATTPVILLTALNSEEQIIQGFQIGADDYVTKPF
                     SPRQLAMRIRAVWRRGSTIGEPEPIRQLRVGELMLDVETHEVIHHGAEIALTPLEFKL
                     LHILAANAGRVVTATRLVDYAWGYDGGDVSLLKTHISHIRKKLRLPQSDIGDIRAVAR
                     VGYRLMQDDPARHQLPQSA"
     gene            2251..2625
                     /locus_tag="VFV93_03205"
     CDS             2251..2625
                     /locus_tag="VFV93_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164381.1"
                     /translation="MAFPNKRHSPRTIARDWRPSKATAGQIDPALLLPEIRAGLYTLE
                     QLLDELGEHEQQLLADAAACYDQLATIAHELDGVRRQLSDATEQAATLREAEYLLVAS
                     RAPARPGEHGDSPRSDRLAVQP"
BASE COUNT          543 a          965 c          809 g          444 t
ORIGIN      
        1 accaaccggc agatcgccgc caacctcggc atggccgaac gaaccgtcga cactcacatc
       61 gggaacatcc gccagaagct gcagctcgcc tcacgggcga agatcgcggt ctggggcgtc
      121 gcgagtggcc tggccagcgc cgagtaaagc agccctacgt catccggctc acgactgccg
      181 gcggcattct cctgcgcaaa ctcggccggc tggagtctcg acaatccacg atatgcagca
      241 cagaccgcgc atgtacgtat acgtcccgat cgaacaatta tcgcgccgag aggtcgaaaa
      301 gtaggtgatt ccacggatac acacgcagca agatgatctg taccatcagc ctgcggacaa
      361 tgaattcgct ggggctaatg aatcaggagt gacgccaccc gacgcacttc agtcataccg
      421 caacaacgac gcgtggaccg cgactgggca cctggtccgt gctaaagcac gtggtgtttc
      481 cggccggcga aaatcgtcag gccggttcct atgttccaat cagggaggga cgaatcaatg
      541 agccttcagt ggttcgaggg accagcgcca catttcctga tctcctcgtc cgaagacgcc
      601 accagccagc tatcaaccac cgcgccagag ctgaacgaac cccttccccc atggctggat
      661 atcgccttcc tgaaccggct cgacgccctc aatgcatccg ctaccgctgt cgttggccgt
      721 caggcacaca tcgcccacga tgtcccgatc tccgagtcca tcgcaacctt cgcgcacgat
      781 atccggacgc cgctggcgac gctccacgcc tcgcttgaga tcctggcaga cggcacgccg
      841 cctgacacat gcgaccttca gcatctggcc ggcacgatgc agcgcggcgt gacgtggatc
      901 acccaactgg tcgccgaact cgcccggcag tcagagacct ctcacggaac cggttgctgg
      961 gggacgccgg tcacgtccgg cccgaccgag gtccgcgact ggatcgagca ggcgattgag
     1021 atcgtccagc cgctcgccgg cactcgcgaa caggcgatcc tgttctcctg cccgcagcca
     1081 tcgccggtcg tcgaaggtga ccacgtccag ttcggacagg tcatcgtcaa cctgctaacc
     1141 aacgcctgcc gctatggcag ctggggagac acgatcgccg tctcggtcag cgagcgcctc
     1201 gaaacggtaa cgatccgtgt ctctgaccac gggatgggca tcctgcccga cgagcgcgag
     1261 aggatcttcg agcgccgcgc ccggggcacc cagaccggca gtcacctcgt tcccggtcag
     1321 gggctcggcc tctacatcgt acggggcatc gtcgagcggc acggcggcag cgtcaccgtc
     1381 gagagcgcgc tcggccaggg atccacattc gccgtccacc tgccggctgc ccgaccggtc
     1441 cagccgttgt tcctgcgccg tgccgtagta cgtgaagatg gtgcgatcga atgaaagtgc
     1501 tgctcgttga cgacgacatc gacctgctgg atgtcacctc gtacgcactc aggcgcgaag
     1561 gcttcaacgt catcgtcgca accgacggcc tccaggccgt tcagcgctgg gaatccgacc
     1621 ggcccgacct catcgtctgt gacatcagca tgccgcggat gaacgggctc gagctctgcc
     1681 agcgcattcg tcgcgacgcg acgacgccgg tcatcctgct gacggcgctg aacagcgaag
     1741 agcagatcat ccagggcttc cagatcggcg ccgacgacta cgtcaccaag ccgttcagcc
     1801 cgcgccagct ggcgatgcgc atccgggccg tctggcgacg cggcagcacc atcggcgagc
     1861 ccgagccgat ccgtcagttg cgcgtcggag agctgatgct cgatgtcgaa acccatgagg
     1921 tcatccacca cggggccgaa atcgccctga ctccgctgga attcaagctg ctgcacatcc
     1981 tggccgccaa cgccggtcgg gtcgtgacgg caacccggct cgtcgactat gcctggggct
     2041 acgacggcgg cgatgtctcg ctcctgaaga cccacatcag ccacattcgc aagaagctgc
     2101 gcctgccaca gagcgacatc ggcgatatcc gcgccgtcgc acgggtcggc taccggctga
     2161 tgcaggacga tcctgcccgg caccaactgc cgcagtccgc ctgagatcgc tgccagtccg
     2221 ccagctagcc gaatgcgagg acaccagcag atggcatttc ccaacaagcg tcattcgccc
     2281 aggaccattg ctcgtgactg gcgaccgtca aaagcgacag ccggccagat cgatcctgcc
     2341 ctgctcctgc cggagattcg agccggcctc tacacgcttg agcagttgct ggacgagttg
     2401 ggagagcacg agcaacaact gctggctgac gccgccgcct gctacgacca gctggcgacc
     2461 atcgcccacg aactcgatgg cgtccgccgt caactcagcg atgcgactga gcaggcagcc
     2521 acactacgcg aggcggagta cctgctggtc gcttcgcgcg cgccagctcg ccccggagag
     2581 catggtgact ccccgcgctc cgacaggctg gccgtccagc cgtagcggtg gacaacgatc
     2641 ccctcgccgg cggtattgca cagccgcgac gccggctgca ggcgctcaac aagacctcaa
     2701 caccaactag actggcaagc cacaccggat ccgtaatctt gccgtgtgag ttccgcttta
     2761 c
//
LOCUS       DASUGJ010000144         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1129917, whole genome shotgun sequence.
ACCESSION   DASUGJ010000144 DASUGJ010000000
VERSION     DASUGJ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129917"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VFV93_03210"
     CDS             <1..847
                     /locus_tag="VFV93_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX5164382.1"
                     /translation="VIVEPMPRGSGPAIGLGVAIAARRDPEAIVGSFAADHIVTRPER
                     FEAAVRAAIETARQGYLVTIGIQPSYPETGYGYICAGEPIGKHHGLTVSSVREFKEKP
                     DVITAARYVESGEYLWNASMFVWQARTLLDEIRRHLPDLADALETIASAWDTPNQPQV
                     MAEVWPSIRDVTIDNGILEKSDRVAVVPGNFGWTDLGDWHGFGTVTCDDPNANSAVNA
                     ELLASDVQGAVVMGNGRLVALLGVRDIVVVDTDDALLICDRSRAQEVRELVEALKQRG
                     STDLI"
     gene            899..3202
                     /gene="topA"
                     /locus_tag="VFV93_03215"
     CDS             899..3202
                     /gene="topA"
                     /locus_tag="VFV93_03215"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX5164383.1"
                     /translation="MTVEKPTRTTSAAKGTRTTKKAGASTTTRKKSTTSKATSRSAPS
                     RRTSSGGKLVIVESPAKARTIERYLGRGYNVRASMGHVRDLPKSKMGIEVDDDFTPTY
                     RVPNDKLKTVKELKDLVKGASEVILATDPDREGEAIAWHLIQATLPEGGNVSRVVFHE
                     ITPDAVRNAMQNPRSIDMDLVDAQQARRVLDRLVGYSISPLLWKKVKRGLSAGRVQSV
                     ALRLVVEREREIQEFVPREYWSLDAELAQQNQQSKKLQSFLAGLSKIRGEKAELPNAW
                     VTDEVVRGLDGAQYVVADVQTKETQRRPAAPFITSTLQQEASRKLNYPVRRTMQIAQE
                     LYEGVDLGPEGTQGLITYMRTDSTNIAASAQAQARTAIARIYGDQFVPERPPVYTRKA
                     KGAQEAHEAIRPTDPNRHPDSLKSVLSAQQLRVYRLIWQRFIASQMRNAIFDSTGVDI
                     DAGPADGEKPYRFRATGSVIKFQGFLAVYREGRDDDEVDEMDKDALPQLTAGEILDLV
                     KLIPEQHFTQPPPRFTEATLVKALEENGIGRPSTYAPTIATLAARNYVTAEQRRLVPT
                     ELGFVVSDILVEHFPSIVDIGFTSEMEEELDEIASGERKWVPVIREFYGPFSESIGKA
                     EQTMERVKVRDEPSDEICDLCGRQMVIKLGKYGRFLACPGFPECRGTKQLLTKIGVTC
                     PKCHEGDIVERRTKKGRVFYGCSRYKTDDPTGCDFSAWSKPTGENCPVCGGAMLVFGK
                     AGTEAKCQDCDHRQPIRPSGVRESIPA"
BASE COUNT          629 a         1052 c         1062 g          488 t
ORIGIN      
        1 ggtcatcgtc gagccgatgc cgcgcgggag cgggcctgcc attgggctcg gcgtcgcgat
       61 cgccgccagg cgcgatccgg aagcgatcgt cggttcgttc gccgccgacc acatcgtcac
      121 ccgtccggaa cggttcgaag cggctgtccg ggccgcgatc gagacggccc gtcaggggta
      181 tctcgtgacg atcggcattc agccgagcta tcccgagacc ggctacggct acatctgcgc
      241 cggggagccg atcggcaagc accacgggct gaccgtctcg tcggttcggg aatttaagga
      301 gaagcccgac gttataactg ctgcacgtta cgtcgagtcg ggggagtatc tctggaacgc
      361 gagcatgttc gtctggcagg cgcggacact gctggacgag attcgccgcc accttcccga
      421 cctcgccgat gcgctcgaaa cgatcgcgtc ggcctgggac acgccgaacc agccgcaggt
      481 gatggccgag gtctggccgt cgatccggga tgtcacgatt gacaatggca tcctggagaa
      541 gtcggatcgt gtcgcggttg tgccgggcaa cttcggctgg accgacctgg gagactggca
      601 cgggtttggc accgtgacct gcgacgaccc gaacgccaac tcggctgtca acgccgaact
      661 gctggccagc gatgtgcagg gcgcggtcgt gatgggcaac ggccggctgg tggccctgct
      721 cggagtgcgc gacatcgtcg tcgtcgacac cgatgacgcg ctgctgatct gcgatcgctc
      781 gcgcgctcag gaggtccgcg aactggtcga ggcgctcaag cagcgcggct cgacagactt
      841 gatatagtga aatgtactgc ctgaaacgtg tacgtacatt ggaaccagtc aggggatcat
      901 gacagtagaa aagccaacca gaacgaccag cgccgccaaa ggcacgagga cgaccaagaa
      961 agccggcgcg tccacaacga cgcgcaagaa gagcacgacg agcaaggcga ccagccgctc
     1021 ggcgccttcg cggcggacat ccagcggcgg caagctcgtc atcgtcgagt cgccggccaa
     1081 ggcgcggacg atcgagcgct atctcgggcg tggctacaac gtgcgtgcgt cgatggggca
     1141 tgtccgcgac ttgccaaaga gcaagatggg tatcgaggtc gacgacgatt tcacgccgac
     1201 ttaccgcgtg cccaacgaca agctcaagac agtcaaggag ctgaaggacc tcgtcaaggg
     1261 cgccagcgag gtcatcctcg cgactgaccc cgaccgcgaa ggcgaggcga tcgcctggca
     1321 cctgatccag gcaacattgc cggaaggcgg caacgtcagc cgcgttgtct tccacgaaat
     1381 cacgcccgac gcggtccgca acgcgatgca gaatccgcgc agcatcgaca tggacctcgt
     1441 cgatgcccag caggcgcgcc gtgtcctcga ccggctggtt ggctactcga tttcgccgct
     1501 gctgtggaag aaggtcaagc gtggcctctc ggccgggcgc gtccagtcgg tcgcgctgcg
     1561 gctggttgtc gagcgcgagc gggagatcca ggagttcgtc ccgcgcgagt actggtcgct
     1621 cgatgccgaa ctggcccagc aaaaccagca gagcaagaag ctgcaatcat tcctggccgg
     1681 cctgtcgaag attcgtggcg agaaggccga gctaccgaat gcatgggtga cagacgaggt
     1741 cgtgcgcggc ctcgacggcg cgcaatatgt cgtcgccgat gtccagacca aggagaccca
     1801 gcgccgcccg gccgcgccgt tcatcaccag cacactccag caggaggcgt cgcgcaagct
     1861 caactatccg gtccgccgga cgatgcagat cgctcaggaa ctctatgaag gtgtcgatct
     1921 cggcccggaa ggaacccagg gtctgatcac ctacatgcga accgactcga ccaatatcgc
     1981 cgcctcggcc caggcacagg cgcggacggc gatcgcgcgc atctacggtg accagttcgt
     2041 gccggaacgg ccgcccgtct atacacgcaa ggcgaaaggc gcccaggagg ctcacgaggc
     2101 gatccggcca accgatccca atcgccaccc ggactcgctc aagagcgtgc tgagtgccca
     2161 gcagttgcgt gtctaccggc tgatctggca gcgcttcatc gccagccaga tgcgcaacgc
     2221 gatcttcgac agcaccggcg tcgacatcga tgccggcccg gccgacggag agaagccgta
     2281 ccgcttccgg gcgaccggct cggtcatcaa gttccagggc ttcctggccg tctaccgcga
     2341 ggggcgcgac gacgacgagg ttgacgagat ggacaaggac gccttgccgc aactgacggc
     2401 cggtgagatc ctcgacctgg tcaagctcat tccggagcag cacttcaccc agccgccgcc
     2461 ccgtttcacc gaggcgacgc tggtcaaggc gctggaggag aacggcatcg gccggccaag
     2521 cacctacgcg ccgacgatcg cgacgctggc cgcccgcaac tacgtcacgg ccgagcagcg
     2581 gcggttggtg ccgaccgagc tgggcttcgt cgtcagcgac atcctcgtcg agcacttccc
     2641 ctcgatcgtc gacatcggct tcacctccga gatggaggag gagctggacg agatcgcctc
     2701 cggcgagcgc aagtgggtgc cggtcatccg cgagttctac gggccgttct ccgagtcgat
     2761 cggcaaggcc gagcagacga tggagcgcgt caaggtgcgc gacgagccga gcgacgagat
     2821 ctgcgatctc tgcggtcgcc agatggtcat caagcttggc aagtacggcc gcttcctggc
     2881 ctgccccggc ttcccggagt gccgtggcac gaagcagctg ctgaccaaga tcggggtcac
     2941 ctgcccgaag tgccacgaag gcgacatcgt cgagcgacgc accaagaaag gccgcgtctt
     3001 ctacggctgc agccgttaca agacggacga cccgaccggc tgcgactttt cggcctggtc
     3061 gaaaccgacc ggtgagaact gcccggtctg cggcggcgcg atgctcgtct tcggcaaggc
     3121 cggcaccgag gcgaaatgcc aggactgcga ccaccgccag ccgatccgtc ccagcggcgt
     3181 ccgcgaatca atcccggcct agaaccagcg ggtaggggcg atggccgcag c
//
LOCUS       DASUGJ010000145         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_853293, whole genome shotgun sequence.
ACCESSION   DASUGJ010000145 DASUGJ010000000
VERSION     DASUGJ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853293"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..2595)
                     /locus_tag="VFV93_03220"
     CDS             complement(193..2595)
                     /locus_tag="VFV93_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX5164384.1"
                     /translation="MRWGAMLEYFQEIAGQSDRVHYEQLGTATEGQPFVLLTISSPEN
                     LGKLDRYREIQARLADPRGLADDEAVRLAAEGRCVVLITCSIHATEVGAVQMTPELVY
                     ELATRDDPEVRRILDEVILLLVPSLNPDGMELVGDWYEQTLDTASEGTPPPSLYHTYT
                     GHDNNRDWFMFTQVETRLAIEKVHNVWHPQIVFDQHQMMADGPRYVLPPFIDPYDPNV
                     DPILQQEVALMGQAMAADLTAAGKAGVATNIIFDAYSPSRAYQHYHGGVRILSEAASV
                     RIATPITLTGEQIRETRGFDPKTASWNHPLPWEGGDWRLRDIVEYNKIAALALLNNAA
                     AYRDRWVRNFWKIAKHTVETDQPQAFVIPSGQRDPVTAAEMLEVLQTGGVEIDEATAS
                     FTADGVEYPAGSYVIPVAQPYGRFAKTMLEGQQYPDLRLYPGGPPKPPYDITAHSLPL
                     QMGVEAFQIEAPFDAALRRVDAVALPDGGVQGTGPCYLIDPRVNASARAVNLLLGIGA
                     RVSRLAASSDGFEAGTFVVEGVDHDGLTRIARETHVTAVAPEGRLTGELRQLRQPRIG
                     LYRSYRPNGIDEGWTRFILERYDFPFTTLRNRDIRQGDLSAQYDVIVLPHQSPRDILD
                     GNPAREYPPEFAGGIGDLGVIQLRRFVEAGGTLIALDAACELAIKQLYLPVTNVLEGV
                     RNDTFYNPGSLLRLLLDPEHPLAWGYEREAAALFVNSPAFDVPNGGNGAQVVGRYPFS
                     NQLLAGWMLGGELIRGRASLVDVPVGKGRVVLFGFRPQFRAQARGTYRLLFNALYYAA
                     ME"
BASE COUNT          435 a          943 c          950 g          468 t
ORIGIN      
        1 cgacatgctg accaaccgga tcacctgtgt acatctctac ccactcgtca tactccacct
       61 gcagggcggg aacgagtcgt acgatccagt cgttcatcgt gtcgtatgtc atggccgaca
      121 tcgaactctc ctacgccagg gatgttcgcc actcgattct gcggttcagg tccattaaca
      181 gaaggcagaa ccttactcca tcgccgcata gtacagcgcg ttgaacagca gccggtacgt
      241 tccccgggcc tgcgcgcgga actgcgggcg gaagccgaac aggacgacgc ggcctttgcc
      301 gaccgggacg tcgaccaggg aggcccggcc gcggatcagc tcgccgccga gcatccagcc
      361 ggccaggagc tggttcgaga atgggtagcg gccgacgacc tgggcgccgt tgccgccgtt
      421 ggggacgtcg aaggcgggcg agttgacgaa cagcgccgcc gcctcgcgct cgtagcccca
      481 ggccagcggg tgctcggggt cgagcagcag ccgcagcagt gagccagggt tgtagaaggt
      541 gtcgttgcgg acaccctcca gcacgttcgt gaccggcagg tagagctgct tgatggccag
      601 ctcgcaggcg gcgtcgagcg cgatcagcgt cccgccggcc tcgacgaagc ggcgtagctg
      661 gatgacgccg aggtcgccga tcccgccggc gaactcgggc gggtactcgc gggccgggtt
      721 gccgtcgagg atgtcgcgcg gcgactggtg cggcaggacg atcacatcgt actgcgcgct
      781 caggtcgccc tggcggatgt cgcggttgcg caacgtcgtg aaggggaaat cgtagcgttc
      841 gaggatgaag cgcgtccagc cctcgtcgat cccgttcggg cggtaggagc ggtagaggcc
      901 gatccgtggc tggcgcaact ggcgcagttc gccggtcagg cggccctccg gcgcaacagc
      961 cgtcacgtgc gtctcgcgcg cgatccgggt gagtccgtcg tggtcgacgc cctcaacgac
     1021 gaacgtcccg gcctcgaagc cgtcgctcga tgcggccagc cgcgagacgc gcgcgccgat
     1081 ccccagcagc aggttgacgg cccgggcgct ggcgttgacg cgcgggtcga tcaggtagca
     1141 cgggccggtg ccctggactc ctccgtccgg cagcgcgacc gcgtcgacac gacgcagcgc
     1201 ggcgtcgaac ggcgcctcga tctggaaggc ctcaaccccc atctggagcg gcaacgagtg
     1261 ggcggtgatg tcgtagggcg gcttcggcgg cccgccgggg tagaggcgca agtcggggta
     1321 ctgctgcccc tccagcatcg tcttggcgaa ccgcccgtac ggctgggcga cagggatcac
     1381 gtacgacccg gccgggtact cgacgccgtc ggccgtgaag gacgccgtcg cctcgtcgat
     1441 ctcgacgcct ccggtctgca gcacctccag catctcagcg gctgtgaccg ggtcgcgctg
     1501 gccggagggg atcacgaacg cctgcggctg gtccgtctcg accgtgtgct tcgcgatctt
     1561 ccagaagttg cgcacccagc ggtcgcggta ggcggcggcg ttgttcagca gcgccagcgc
     1621 cgcgatcttg ttgtattcga cgatgtcgcg cagccgccag tcgccgccct cccacggcag
     1681 cgggtggttc caactggccg tcttcgggtc gaagccgcgc gtctcgcgga tctgctcgcc
     1741 cgtcagcgtg atcggcgtcg cgatccggac gctggccgcc tcggagagaa tccggacgcc
     1801 gccgtggtag tgctggtagg cgcgcgaggg cgagtaggcg tcgaagatga tgttggtggc
     1861 aacgccggcc ttgccggccg ccgtcagatc ggccgccatc gcctgcccca tcagcgccac
     1921 ctcctgctgg aggatcgggt caacgttcgg gtcgtagggg tcgatgaacg gcggcaggac
     1981 gtagcgcggg ccgtcggcca tcatctggtg ctggtcgaag acaatctgcg ggtgccagac
     2041 attgtggacc ttctcgatcg ccagccgtgt ctcgacctgg gtgaacatga accagtcgcg
     2101 gttgttatcg tggccggtgt aggtatggta gagcgacggc ggcggcgtcc cttcggaggc
     2161 cgtatcgagc gtctgctcgt accagtcgcc gaccagttcc atcccgtccg ggttgagcga
     2221 cggcaccagc agcaggatca cctcatcgag gatgcgccgc acctcgggat cgtcccgcgt
     2281 tgccagctcg tagaccagtt ccggcgtcat ctggaccgcc ccgacctcgg tcgcgtggat
     2341 cgagcaggtg atcaggacga cgcagcgccc ctcggccgcc agcctgacgg cctcgtcgtc
     2401 ggccagcccg cgcgggtcgg ccagccgcgc ctgaatctcg cggtagcggt cgagcttgcc
     2461 caggttctca ggcgaggaga tcgtcagcag gacgaacggc tgcccctcgg tcgccgtccc
     2521 cagctgctcg tagtggacgc ggtcgctctg gccggcgatc tcctggaagt actccagcat
     2581 cgccccccag cgcaccaact tccggtctgt ccccatctga aacccaaagc gctcatccgg
     2641 tgtccgcaca accatccccc atccgcgatt ccgagatcct gattccgatc gtacccgatc
     2701 gcgccgtgca atcaagcgcc gacgcgtcgt atcatgcact gcattcagtc tttcccttgg
     2761 ccgaggattg cgccgatgcc gttgtcgaac aactcc
//
LOCUS       DASUGJ010000146         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1548393, whole genome shotgun sequence.
ACCESSION   DASUGJ010000146 DASUGJ010000000
VERSION     DASUGJ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548393"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFV93_03225"
     CDS             <1..412
                     /locus_tag="VFV93_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164385.1"
                     /translation="DVEGIGRPRVEPSFIRTVVDEMIKVPDEASYAAMLFLYKLMAKR
                     AGPSTGTNLWGAFELIADMTAAGERGSIVSLICDSGERYSHTLYNSEWLAGRQIDPGR
                     WQRQLQTFVDSGTWLVRQPRAVREPDASIVGRTL"
     gene            complement(516..>2004)
                     /locus_tag="VFV93_03230"
     CDS             complement(516..>2004)
                     /locus_tag="VFV93_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5164386.1"
                     /translation="FTAQFLLTLTGGVAGVLIAWGGTLLVRQVGGGLERLDLIRGNRM
                     LPRVDEIGIDGSVLLYTAALVAVTAIACSAGAIWRLLRLSPGRILASADAPASTLGRA
                     TPLLLSAQVALTVVLMVSALLLTRSFTNLSHIDPGYDATGVLTFQVVPRDAESAVEFA
                     TWGKRQVEVADRLVARLQQLPGVTAAGFTSNLPFNEGSYQLSIATARPGPATSIDQGR
                     GVVVSPGYFQAMHMPIVAGRGFVADDAQRSTASFVINQTLVRRYFGGADPLGRRVYVW
                     GIAGDIIGIVGDAHATTLDVAAQPQLYLTPYRGQPYLPLLSDGLYFTLRATNAMALVP
                     MIRDAAREIAPDAPVQRMSMLADIVANSLRADQASAWIVSIIASGALALVAIGLYGLV
                     AYLVAQRTREIAIRLALGARHDQVQALVMRGSLTAVVTGVLAGLVMSAVAAPALRGLL
                     FGVGASDPSSFAAASVLVVGIALTACALSARRAAAIEPMTLLRSN"
BASE COUNT          383 a          683 c          652 g          286 t
ORIGIN      
        1 cgatgtcgaa gggattggac ggccgcgggt cgagccatcc ttcatccgga cggtcgtcga
       61 cgagatgatc aaggttccgg acgaggcctc gtacgcggcg atgctctttc tgtacaagtt
      121 gatggcgaaa cgggccggtc cctcgaccgg gacgaacctg tggggcgcct tcgaactgat
      181 cgcggacatg acagctgccg gcgagcgtgg ttcgatcgtc tcgttgatct gcgactcggg
      241 cgagcgctac tcccacacgc tctacaacag cgagtggctg gccggccgcc agatcgaccc
      301 tggccggtgg caacggcaac tgcagacgtt cgtcgacagc ggcacgtggc tcgttcgcca
      361 gccgcgcgcc gtccgagagc ctgacgcctc gatcgtaggg cgaacccttt aagtcgcggg
      421 aaggccgtga tcgtaggcgg accttgtcag gtccccggga cgggcgtgat ggcacggcga
      481 accttgtcag gtctgccagc cgcgcgtgat acacgtcaat tgcttcgcag cagcgtcatc
      541 ggttcgatgg cggccgcccg gcgggccgac aaggcgcacg ccgtcagcgc aattcccacg
      601 acgagaaccg acgccgccgc aaagctggac gggtcggatg ctccgactcc gaacagcaga
      661 cctcgcagcg ccggcgcagc cacggccgac atcacgagtc cggcgagcac gccggtgacg
      721 acggctgtca ggctgcctct catcaccagc gcctgcacct gatcgtgacg agcaccaaga
      781 gccagccgga tggcgatctc gcgcgtccgc tgcgccacca ggtaggcaac cagcccgtac
      841 agcccgatcg cgacgagcgc gagcgcccct gacgcgatga tgctgacgat ccacgcggat
      901 gcctggtcgg cgcgcagcga attggccacg atgtcggcca gcatcgacat gcgctgaacc
      961 ggcgcatcag gagcaatctc gcgggcggcg tcgcgaatca tcggcaccag cgccatcgca
     1021 ttcgtcgcgc gcagcgtgaa gtacagcccg tccgagagca gcggcagata cggctgtccg
     1081 cgatacgggg tcaggtacaa ctgcggctgc gcggcgacgt cgagcgtggt ggcgtgagca
     1141 tcaccaacga tcccgatgat gtcgccggcg attccccaca cgtacacccg ccggcccagg
     1201 ggatccgcac caccgaaata gcggcggaca agagtctggt tgatcacgaa cgatgccgtc
     1261 gaccgctgcg catcatcagc gacgaatccg cgcccggcca cgatcggcat gtgcatcgcc
     1321 tggaagtagc ctggactcac caccacgccg cgtccctgat cgatcgaggt ggcagggccc
     1381 ggcctggccg tggcaatcga cagctggtac gacccctcat tgaacggaag gttgctcgtg
     1441 aacccggcgg ccgtaacgcc tggcagctgc tggaggcgcg ccaccagtcg atcggcgact
     1501 tcgacctgcc gtttccccca ggtggcgaat tcgacggccg actccgcatc gcgcggaacc
     1561 acctggaacg tcaggacgcc ggtggcatcg tacccgggat cgatgtggga gagattcgtg
     1621 aagctccgcg tcaagagcag cgcactcacc atcaacacca cggtcagcgc gacctgcgcc
     1681 gagaggagga gcggggtggc gcgcccgagt gtcgacgccg gcgcatccgc actggcaagg
     1741 atgcggcccg gcgacagtcg caacaatcgc cagatcgctc cggcactaca ggcgatggcg
     1801 gtcaccgcga ccagagcggc ggtatacagc aatacggagc catcgatccc gatctcatcg
     1861 acgcgcggga gcatccggtt cccgcgaatc agatccagtc gctcgagccc accacccact
     1921 tgccgaacca gcagcgtacc gccccaggcg atcagcacac cggccacacc gccggtcagc
     1981 gtcagcagga actgggcggt gaac
//
LOCUS       DASUGJ010000147         7824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1686874, whole genome shotgun sequence.
ACCESSION   DASUGJ010000147 DASUGJ010000000
VERSION     DASUGJ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686874"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2649)
                     /locus_tag="VFV93_03235"
     CDS             complement(<1..2649)
                     /locus_tag="VFV93_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164387.1"
                     /translation="METNAVRERLQSARERGVPWKHWGPYLSERQWGTVREDYSENGD
                     AWSYFPHDHARSRAYRWGEDGLLGISDDKQRLCFALALWNGADPILKERLFGVTGPEG
                     NHGEDVKEYYYYLDATPTHDYLKGLYKYPQDAYPYLDLLETNRRRTRDEMEYELIDTG
                     IFNASRYFDVVVEYAKSSPEDIAVRITAHNRGPEAAPLHLLPTLWFRNTWSWGGEVDR
                     PQLRAGMASGDVQVVAVTHPELGSRWLYCAGSPELLFTENESNAERLWGQPNRTRYVK
                     DGINNAVVHGQTVAVNPERTGTKVAANYALVIPAGEHAHVYLRLSDVGPALIGDPFDD
                     IAGLFNRRLDDADAFYATLTPAGASSDEASVMRQALSGMIWTKQYYGFNLASWLEEHD
                     ASPLSAAPRQIRNREWFHMLNDDIISMPDKWEYPWYAAWDLAFHTVPLVMVDPDFAKQ
                     QLELLLRELYLHPNGQVPAYEWNFSDVNPPVHAWATMFVYLMEQRTRGEGDLTFLRSS
                     FHKLLMNFTWWVNRKDPTGRNVFEGGFLGLDNIGVFDRSAQLPTGGHLEQADGTAWMA
                     FYSQCMLDIALELAAHDSLYEDYALKFVEHFFWIAGAMDRLGDQHDEIWDEEDGFFYD
                     VLRLPDGRATRIKVRSMVGLLPLCATTLFPVSTLERYPALVSRIRQFLDRHPELRKVV
                     PAPLQPGPDGRRILTSLDEDKLRRVLHRMLDEDEFFSPYGIRAISRAHLDEPYVVNVH
                     GEEFRVDYWPAESHNAMFGGNSNWRGPIWFPVNALIYRALLQYYGFYGDDFKIECPTG
                     SGNQMTLFEVANELARRLSAIFLRDSDGRRPVYGGAETFQNDPHWRDHILFYEYFHGD
                     NGAGLGASHQTGWTGLVAPLILLQHVA"
     gene            3069..4307
                     /locus_tag="VFV93_03240"
     CDS             3069..4307
                     /locus_tag="VFV93_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5164388.1"
                     /translation="MSELDATESLIPRLRRLTPAELAVLRRRCEGMTAQETASAFSTT
                     EQTARYLLGNIYDKLGITYDDGVGTSLSRLMEYCPLLADPSISQIGSPASLENEPLPL
                     EPSGRALLLVAQDDQALLAQRTPTPEADSSGNNRRLLYLLGGALAVAVIGGLIYLLMS
                     DDDGDDAAAAATQTATISTTATVAPEATTSTGGSVTEPAATLAPSPTPEPSPTPEPAA
                     TDVPPEATAVPQPLQGEVVYQADWSTGPGDWVLPDGWTAQSGTLAGDGATAGSILAPF
                     QSDQPRYAVEADIVIDGTPSCDALAGVIARAANGDGVLTAGYAGAVCTDEWLILAVRE
                     RSDVPDELASGEIEPITGQHTYRLEVDGERLRFFIDAVFAGETEDVRWADPGSAGLFL
                     DGDLRVTVAGFRVYSLPETP"
     gene            complement(4466..5380)
                     /gene="arcC"
                     /locus_tag="VFV93_03245"
     CDS             complement(4466..5380)
                     /gene="arcC"
                     /locus_tag="VFV93_03245"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010635806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX5164389.1"
                     /translation="MRVVVALGGNALLRRGEPMTADVQRENVRIAAQALAPVAERHEL
                     VISHGNGPQVGLLALQAHAYGDVEPYPLDVLGAETEGMIGYMLELELGNLLPFEVPLA
                     TLLTMIEVDPEDPAFKDPTKFVGPVYDKAHADKLAADKGWAFKQDGEQWRRVVPSPLP
                     RRIFELRPIKWLLEHDTIVICSGGGGIPTAYGPNGARTLRGVEAVIDKDLASELLARE
                     LDADVFVMATDVDGVYLDYGTPRQRMLEQVTASEIAGYGFAAGSMGPKVDAAIKFAVS
                     TGRRSAIGTLRDIEEIVEGSKGTNIVAG"
     gene            complement(5449..>7824)
                     /locus_tag="VFV93_03250"
     CDS             complement(5449..>7824)
                     /locus_tag="VFV93_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5164390.1"
                     /translation="VELPAEQLVPGDIVTFEAGDKVPADGRLLTAATLEIEEAGLTGE
                     STPVLKSTASVTGDDVPLGDRLDMAYMNTTVTRGRGEMITTTTGMSTEMGRISDMLTT
                     VEEEKTPLTRQLDQLTVMITGAAGVALILVVLIGLARDQSFDEMFLLGISLAIAAIPT
                     GMPAVVTTLLSMGTQKLAEQGAIVKRLRSVETLGATSAICSDKTGTLTLNQMTARELI
                     VPGRKYKIEGEGYSTEGKILRVAGDGDPAFEPIMLPMALCSDATVTDGNIVGDPTEAA
                     LVVLAAKGGYDVPETRKRYPRVAEVPFDSDYKFMATFHRIQTDDGSPVVRCFVKGAPD
                     VLLSRSTTGLGAAGTPEDIDPYRARVMEENDRLASEGLRVLAVGYRDFDSTTFDSSAN
                     LLDEIKDLTLVAFVGIVDPPRKEARDAIAHCKTAGIRVRMITGDHVTTAAAIAQQLGI
                     EGRAIMGTEFAAMSDEELNAQVDNIGVVARVAPEDKVRLVEVLKRRGNVVAMTGDGVN
                     DAPALKRSDIGVAMGITGTEVTKEAAEMILNDDNFATIVKAVDSGRGLYDNLMKYVRF
                     QIVALTGFILLFVLAGIFNVAEGIPLTPLQILWVNFAIDVMLAVGLGFDAATPGIMER
                     KPRPADAPILTRALGTRLVTGGLIMAVATLGVVAIGEDRYALPVALTMGLVTLSLLHI
                     VAAVSTRSPERHILSRFTISNSRFLILILASVVLTLLVTEISLLQRMFDTVALTSEQW
                     GICLLATGIVMVVLELSKVLLNRTGWASVAAASVDLEESPTSMVPGAQPAS"
BASE COUNT         1389 a         2434 c         2526 g         1475 t
ORIGIN      
        1 ggcgacgtgc tggagcagga tcagcggcgc gaccaggccg gtccagccgg tctggtggct
       61 ggcgccgagt ccggcaccgt tgtcgccatg gaagtactcg tagaacagga tgtggtcgcg
      121 ccagtgcgga tcattctgga aggtctctgc gccgccgtag accggtcgcc gtccgtcgct
      181 gtcacggagg aagatggcgg acagccgccg cgccagttca ttggcgacct cgaagagcgt
      241 catttgattg ccggagccgg tcgggcactc gatcttgaag tcgtcgccgt agaagccgta
      301 gtactggagc agggcgcggt agatcagagc gttgactggg aaccagatcg ggccacgcca
      361 gttggagttg ccaccgaaca tcgcgttgtg cgattcggcc ggccagtagt cgacgcggaa
      421 ctcctcgccg tgcacgttga cgacgtacgg ctcgtcgaga tgggcgcgtg agatcgcgcg
      481 aatgccatac ggactgaaga actcgtcctc gtccagcatg cggtgcagca cgcgccgtag
      541 cttgtcctcg tccagcgatg tcaggattcg ccgcccgtct ggtccaggct ggagcggggc
      601 agggacgacc ttgcgaagct ccgggtgcct gtcgaggaat tgtcggatgc gcgacaccaa
      661 tgccgggtac cgctccagcg tcgaaaccgg aaacagcgtt gtggcacaga ggggcagcag
      721 gccgaccatc gagcgaactt tgatacgcgt tgctctgccg tcaggcaggc gcaggacgtc
      781 gtagaagaag ccgtcttcct catcccagat ctcgtcgtgc tggtcgccga ggcgatccat
      841 cgcaccggcg atccagaaga agtgctcgac gaacttgagc gcgtagtctt cgtagagaga
      901 gtcgtgcgcg gccagttcga gcgcgatgtc aagcatgcac tggctgtaga acgccatcca
      961 ggcggtgccg tcggcctgct ccagatgccc acccgtcggt agctgtgcgc tgcgatcgaa
     1021 gacaccgatg ttgtccagcc cgaggaagcc accttcgaag acattgcggc cggtcgggtc
     1081 cttccggttg acccaccagg tgaagttcat cagcagcttg tggaacgagc tacggaggaa
     1141 cgtcagatct ccctcaccgc gcgttcgctg ctccatcagg tagacgaaca tcgtcgccca
     1201 ggcgtggacg ggcgggttga cgtcgctgaa gttccactcg taggccggca cctgaccgtt
     1261 gggatgcagg tagagttcgc gcagcagcag ttcaagctgc tgcttggcaa agtccggatc
     1321 gaccatcacg agtggaaccg tgtggaacgc caggtcccag gcggcgtacc acgggtattc
     1381 ccacttgtcc ggcatcgaga tgatgtcgtc gttgagcatg tggaaccact cgcggttgcg
     1441 gatctggcgc ggggccgcgc tgagcgggga ggcatcgtgc tcttccagcc agctggccag
     1501 gttgaagccg tagtattgct tggtccagat catccccgac agcgcctggc gcatcacact
     1561 ggcctcgtcc gagctcgcgc cagcaggagt cagggtcgca tagaacgcgt cggcgtcgtc
     1621 aagacgacgg ttgaacaatc ccgcgatgtc atcgaacgga tcgccgatca gtgccggccc
     1681 gacgtcactc aggcgcagat agacgtgcgc gtgttcgccg gcggggatga cgagcgcgta
     1741 attggccgcg actttcgttc cggttcgctc aggattgaca gcgaccgttt gcccatgaac
     1801 gacggcgttg ttgatgccgt ccttgacgta tcgcgtccga ttgggctggc cccagagccg
     1861 ctccgcattg gactcattct cggtgaacag gagttccggc gagccggcac agtacagcca
     1921 gcggcttcct agttcagggt gcgtgacggc aaccacctga acatcgccgg acgccattcc
     1981 ggcgcggagc tgcgggcgat ccacctctcc gccccatgac caggtgttgc ggaaccagag
     2041 cgtcggcagg aggtgcagcg gggcggcctc cgggccacga ttgtgggcgg tgatgcggac
     2101 ggcgatgtcc tcgggagatg acttggcata ttcaacgacg acatcgaagt agcggctcgc
     2161 gttgaagatc ccggtgtcga tcagctcgta ctccatctcg tcacgtgtgc gacgccgatt
     2221 ggtttcgagg agatcgagat acggataggc gtcctgcggg tatttgtaga gacctttgag
     2281 gtagtcgtgc gtcggcgttg cgtcgaggta gtagtagtat tccttgacat cctcaccgtg
     2341 gttaccttca gggccggtca ccccaaacag gcgttccttg aggatcggat cagctccgtt
     2401 ccagagcgcc agggcgaagc agaggcgttg cttgtcatcg gaaatgccga gcagcccgtc
     2461 ctcgccccag cgataggccc gtgagcgggc atggtcgtgt ggaaagtagc tccaggcgtc
     2521 gccgttttcg gagtagtcct cgcggacggt cccccattgg cgttcggaga gatatggtcc
     2581 ccaatgcttc cagggcaccc cacgctcacg cgcgctctgc aggcgctcgc ggaccgcgtt
     2641 cgtctccatc tgtccctccc tgggttccga cggcgaacct gcctctcggc gcaagcggat
     2701 gcacgctcca gtggctcgcc gtggatacgg gcgattcgta ccaactcgat catccgggac
     2761 gagaacaccg gcagcatggt ccggccagat gtatttgcag ggcgcaatat gaccgggttc
     2821 tcgttggcag atttcgtgac gcccggttcc gcttcagggc gtgattttca ggtcattggg
     2881 tggtagtgct gccgtcgcgt cgcggtccgt cgattacaac cggcgactac atccgagcgg
     2941 cgattccgaa cgttggccgt caacgtacct tgaacgcacg aatgccaatg cccgcccgag
     3001 ggcggctgtc gagtcgaaca ctgtttcggg tcgcgacgtt gccgcacaag tggaaattga
     3061 gaccagccgt gtccgagctt gacgccaccg agagtctgat tccccgtcta cgccggttga
     3121 cgccggccga gcttgccgtg cttcgtcgtc gatgcgaagg gatgaccgct caggaaaccg
     3181 ccagtgcctt cagcacgacg gagcagacag ctcgctatct gctgggcaac atctatgaca
     3241 agctgggcat tacctacgac gacggtgtcg gaacgtcgct gtcacggctc atggagtact
     3301 gcccgctgct tgccgatccg agcatctcgc agatcgggtc gccggcgtct ctggagaacg
     3361 agccgttacc gctcgaacca tcggggcgag cgctcctgct cgtcgcccag gacgatcagg
     3421 cgctgctcgc ccagcggact ccgacgccgg aggctgattc atcgggcaac aaccgccggc
     3481 tcctgtacct gctcggcggt gcgctggctg tcgctgtcat tggcgggctg atctatctgc
     3541 tcatgtcgga tgacgatggc gacgatgccg ctgctgctgc aacccagact gcgacgatat
     3601 cgacgacggc cactgtcgcg cccgaggcga cgacctcaac tggtgggagt gtgaccgagc
     3661 cggctgcaac gctcgcgccc agcccaacgc cggagccatc accgacgccc gagccggcag
     3721 cgaccgatgt accgccggaa gcgacggccg tgccacaacc cctgcaaggc gaggtcgtct
     3781 atcaggcaga ctggtcaacg ggaccgggcg actgggtact ccctgacggc tggacagctc
     3841 aatcgggcac gctcgccggt gacggcgcga ctgccggttc gatcctcgcg ccgttccagt
     3901 ccgatcagcc gcgttatgcg gtcgaggcgg atatcgtgat cgacggcaca ccctcgtgtg
     3961 atgcgctcgc cggcgtgatc gctcgtgcag cgaacggcga cggcgtgctg acggccggat
     4021 acgccggcgc ggtctgcaca gacgaatggc tcatcctggc ggttcgcgag cgcagcgacg
     4081 tacccgacga actggcatcg ggagagatcg agccgatcac tggtcagcac acctaccggc
     4141 tcgaggttga tggcgagcga ttacggttct tcatcgacgc tgtcttcgcc ggcgagacag
     4201 aggacgtccg ctgggccgat ccgggatcag cgggactctt tctcgatggc gatctgcggg
     4261 tgaccgtggc tggcttccgc gtctactcgc tccctgaaac tccgtgagcc catgtgaaat
     4321 gtattctaac cataagagtg gcaagctcag gccgatgttt agagatgcgc gcagaacgtt
     4381 gcccctcatc cgctgggctg atcgtgtgta cgtctcgccg gggtgcgctc ggtcgtctcg
     4441 actggataaa cgctgacatc gaatgtcagc cggcgacgat gttcgtccct ttgctgccct
     4501 caacgatctc ctcgatgtcg cgcagtgtcc cgatcgcgga cctccggccg gtcgagacag
     4561 cgaacttgat cgcagcgtcc acttttggac ccatcgaacc agccgcgaat ccgtagccgg
     4621 cgatttccga agccgtgacc tgttccagca tgcgttgccg gggagtgccg tagtcgagat
     4681 agacgccatc aacatcggtg gccattacga acacatcagc atcgagttcg cgggcgagca
     4741 actcgcttgc cagatcctta tcgatgaccg cctcgacgcc gcggagggtc cgagcaccgt
     4801 ttggaccgta ggccgtcgga ataccgccgc ctccagagca gatcacgatc gtgtcgtgtt
     4861 caagcaacca cttgatcggc cggagctcga agatgcgtct gggaagcggg gagggaacga
     4921 cgcggcgcca ctgctcgccg tcctgcttga atgcccagcc cttgtcggct gcaagtttgt
     4981 cggcgtgtgc cttgtcgtag accgggccaa cgaacttggt cgggtccttg aaggccggat
     5041 cttcgggatc gacttcaatc atcgtcagca acgtcgcgag tggcacctcg aatgggagca
     5101 ggttgccgag ctccagttcg agcatgtagc cgatcatgcc ttcggtttcg gcgccgagga
     5161 catcgagcgg gtagggctcg acgtcgccgt aggcatgggc ctggagcgcc agcagcccaa
     5221 cctgcggacc atttccatgc gagatgacga gttcatgacg ttccgcaacc ggcgcaagcg
     5281 cctgggcggc gatgcgcacg ttctcgcgct gcacgtcggc tgtcatgggc tcaccgcgcc
     5341 ggaggagcgc gttcccgcca agggcgacga ctacccgcat gatcttctcc ttcgctgtaa
     5401 ggaacagcgc gatgaggcat tgccccatcg cgctgacgat cctctctgtt agctggccgg
     5461 ctgcgcccct ggaaccatcg acgttggcga ctcttcgagg tcgacagatg cggcagctac
     5521 cgacgcccat ccggtgcggt tgagcagcac cttgctcagc tccaggacga ccatcacgat
     5581 gccggttgcc aggaggcaga taccccattg ctcgctcgtg agcgcaacgg tgtcgaacat
     5641 ccgttgcagc aggctgatct cggtcacgag aagcgtcagc acgactgacg cgagaatcag
     5701 gatgaggaac cgcgaattcg agatcgtgaa ccggctgagg atgtgccgct caggggagcg
     5761 cgtgctgacg gcagccacga tgtggaggag agacaacgtg accaacccca tcgtcagggc
     5821 taccgggagc gcgtatcgat cctcgccgat cgcgacaacg cccagcgtcg cgacggccat
     5881 gatcagaccg ccggtgacca gccgtgtgcc gagcgctcgt gtcaggatcg gggcgtctgc
     5941 cgggcggggc ttgcgctcca tgatgccagg tgtcgcggcg tcgaagccaa ggccaactgc
     6001 cagcatgacg tcgatcgcga agttcaccca gagaatctgt agcggtgtca gcggaatgcc
     6061 ctcggcgaca ttgaagatgc cggccaggac gaagagcagg atgaacccgg tcagggccac
     6121 gatctggaag cgaacatact tcatgaggtt gtcgtaaagt ccgcgtcccg aatcgaccgc
     6181 tttcacgatc gtcgcgaagt tgtcgtcatt gaggatcatc tcggcggctt ctttggtcac
     6241 ttcggtgccg gtgatgccca tcgcgacacc gatgtccgag cgcttgagcg ccggagcatc
     6301 gttgacgcca tcaccagtca tcgcgacgac gttgccgcgc cgcttgagga cttcaacgag
     6361 ccgcaccttg tcttcgggag cgacgcgggc gacgacgccg atgttgtcga cctgggcgtt
     6421 gagctcttca tcactcatgg ccgcgaactc ggtgcccatg atcgcgcgtc cctcgatgcc
     6481 gagttgttgc gcgatcgcgg cggccgtcgt cacgtggtct ccggtgatca tccggacccg
     6541 aataccggcc gtcttgcagt gcgcgatcgc gtccctcgct tccttgcgag gcgggtcgac
     6601 gatccccaca aacgcgacga gggtcagatc cttgatctcg tccagcagat tcgcactcga
     6661 gtcaaaggta gttgaatcga agtcccggta gccgaccgcc agcactcgca acccttcact
     6721 cgccagccgg tcgttctcct ccatgacgcg cgcgcgatat gggtcgatgt cttctggtgt
     6781 cccggccgcg ccaagaccgg tggtcgagcg cgagagcagt acgtccggcg cgcctttgac
     6841 aaagcagcgc acaaccggcg atccgtcgtc ggtctgaatt cggtggaacg tcgccatgaa
     6901 cttgtagtcg gaatcgaacg gcacctcggc gactcgcggg tatcgcttgc gcgtctccgg
     6961 cacgtcgtat ccgcccttgg cggccaggac gacgagggca gcctcggttg ggtcgccgac
     7021 gatgttcccg tcagtgacgg tcgcgtcgct acagagtgcc atcggcagca tgatcggctc
     7081 gaacgccgga tcgccgtcgc cggcgacgcg caggatcttg ccctcagttg agtagccctc
     7141 gccttcgatc ttgtacttgc ggcccgggac gatcaactcg cgggcggtca tctggttcag
     7201 tgtcagcgtg ccggtcttgt cggagcagat tgccgacgtg gcgccgagcg tctcaaccga
     7261 gcgcaggcgc ttcacgatgg cgccctgctc ggccagcttc tgcgttccca tcgacagtag
     7321 cgtcgtgaca acggccggca tgccggtcgg gatcgcggcg attgcgaggc tgatgccgag
     7381 caggaacatc tcgtcgaacg actggtcacg cgcaaggccg atcaggacga cgaggatcaa
     7441 cgcgacgccg gccgcaccgg tgatcatgac ggtcaattga tccagttggc gggtgagcgg
     7501 cgtcttttcc tcttcgacgg ttgtgagcat gtcggagatg cgacccatct ccgttgacat
     7561 accggtcgtg gtcgtgatca tctccccacg cccgcgggtc accgtcgtgt tcatataggc
     7621 catgtcgagg cggtcaccga gcgggacatc gtcgcccgtt accgatgccg tgctcttgag
     7681 gacaggcgtg ctctctcccg tcaggccggc ttcctcgatc tcgagcgtgg cggccgtcag
     7741 caggcgccca tcggccggca ccttgtcgcc ggcctcaaac gtgacgatgt cgccgggtac
     7801 aagctgctcg gcgggcaact cgac
//
LOCUS       DASUGJ010000148         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1965192, whole genome shotgun sequence.
ACCESSION   DASUGJ010000148 DASUGJ010000000
VERSION     DASUGJ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965192"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFV93_03255"
     CDS             <1..538
                     /locus_tag="VFV93_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164391.1"
                     /translation="LLGVAVPSFFLGTILILYGSRYISFIPTLQYVSFREDPKTHLLT
                     MIYPVVSLGTSLAAVIAENTWSAVNDTRNLEHVRVARAKGLPERRVITGHILRNALIP
                     ITTVTGLQIAFLLGGTIIIETIFALPGLGRLAFSAINLRDYPLMQGIVMFIALMVVLA
                     NLLADLAYALFDPRIRVS"
     gene            548..1462
                     /locus_tag="VFV93_03260"
     CDS             548..1462
                     /locus_tag="VFV93_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164392.1"
                     /translation="MASSQVLSDQQAEQAGQLRGSLAARPSFWRRARRKLNPQLSLGL
                     GLLILILIVTIFAPVFTQHDPNTITMTGSLAPPQSGNILGTDSIGRDVFSRVLYGGRV
                     SLSVAVPSVALALLLGLLLGVPAGYMGGKVDQVIMRVLDVFFAFPGVLLALVIVTILG
                     PSIRNLVLTIGILYAPRMARIVRAPTMSTKSLDFVEAARSVGAHDLRIVLRHVLPNVS
                     SPVIVDVSLALGQVILTETALSFLGMGPPPPAASWGSMLSQSRQFMEFASWTVLAPGI
                     AIVITVSSFLLIGHGLRRMLDPRRQTVG"
     gene            complement(1517..2008)
                     /locus_tag="VFV93_03265"
     CDS             complement(1517..2008)
                     /locus_tag="VFV93_03265"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5164393.1"
                     /translation="MAVDGMTLRNDLRTGDLGWIVERHGVLYADEFGWNIEFEAVVAE
                     IIGHVVTGFDPSRERAWIAERDGRRLGSIFLEAGTGTTAKLRLMLVEPDARGLGLGTW
                     LVDECVQFAREAGYSGITLWTMHVLVAARHIYERAGFQLVAAEPGHYFGNDLVSETWE
                     IGL"
BASE COUNT          315 a          741 c          675 g          403 t
ORIGIN      
        1 cctgctgggc gtcgccgtcc cgagcttctt cctcggcacg atcctgatcc tctacggcag
       61 ccgctacatc agcttcatcc cgaccttgca gtatgtcagc ttccgcgaag acccgaagac
      121 gcacctgctg acgatgatct atcccgtcgt ctcgctcggc acgtctctgg cggccgtcat
      181 cgccgagaac acctggtcgg ccgtcaacga cacccgcaac ctggagcatg tccgcgtcgc
      241 gcgggccaag gggctgccgg agcggcgggt gatcaccggc cacatcctga ggaacgcgct
      301 gatcccgatc acgaccgtga caggcttgca gatcgccttc ctgctgggcg gcacgatcat
      361 catcgagacg atcttcgcct tgccgggact ggggcggcta gccttcagcg cgatcaacct
      421 gcgcgactat ccgttgatgc agggaatcgt catgttcatc gcgctgatgg tcgtcctggc
      481 caacttgttg gccgacctcg cctacgcgct gttcgatccg cgaattcgcg tgagttaggg
      541 gacgcggatg gcaagctcgc aggtgctatc agaccaacaa gctgagcagg ccgggcagtt
      601 gcgcggctcc ctcgctgccc gcccgtcgtt ctggcggcgg gcgcggcgca agctgaaccc
      661 gcagttgtcg ctcggactgg ggctgctgat cctgatcctg atcgtgacga ttttcgcgcc
      721 ggtcttcacc cagcacgacc cgaacacgat cacgatgacc ggctcgctcg cgccgccgca
      781 atccggcaat atcctcggga ccgactcgat cggtcgcgat gtcttctcac gcgtcctcta
      841 cggcgggcgc gtctcgctct cggtcgccgt cccgtcggtc gcgctggcgt tgctgctggg
      901 gttgctgctc ggcgtcccgg ccggctacat gggcggcaag gtcgatcagg tcatcatgcg
      961 ggtgctggat gtcttcttcg ccttccccgg cgtcctgctg gcgctggtga tcgtgacgat
     1021 tctcggtccg agcatccgca acctggttct gacgatcggt attctctacg cgccccggat
     1081 ggcacgcatc gtcagggcgc cgacgatgtc gaccaagtcg ctcgacttcg tcgaagcggc
     1141 ccgctcggtc ggcgcgcatg acctgcggat cgtgttgcgc catgtcctgc cgaacgtctc
     1201 gtcgccggtg atcgtcgatg tcagcctcgc gctcggccag gtgatcctga ccgagacggc
     1261 gctgagcttc ctcgggatgg gcccgccccc gccggccgcg tcgtggggtt cgatgctcag
     1321 ccagagccgc cagttcatgg agttcgcctc ctggacggtg ctggcccccg gcatcgcgat
     1381 cgtcatcacc gtctccagct tcctcctgat cggccacggc ctgcgccgca tgctcgaccc
     1441 ccgccgccag acggttggat gagcgggatt cgggattcgg gattcgggac gcgtcacgca
     1501 ctacgcgtcc cgaacgctac aaccctatct cccacgtctc gctgacaagg tcgttgccga
     1561 agtagtggcc cggttcggcg gcgaccagtt ggaagccggc tcgttcgtag atgtggcggg
     1621 cggcgacgag gacgtgcatc gtccagagcg tgatgccgct gtagccggcc tcgcgggcga
     1681 actggacgca ctcgtcgacc agccacgtgc cgagcccgag gccgcgcgcg tccggctcga
     1741 ccagcatcag tcgcagcttg gcggtcgtcc cggttccggc ttcgagaaag atgctgccga
     1801 gacgccggcc atcccgttcg gcaatccagg cccgttcgcg gctgggatcg aagccggtca
     1861 cgacgtggcc gatgatctcg gcgacgacgg cctcgaactc gatgttccag ccgaactcgt
     1921 ctgcgtagag gacgccgtgc cgctcgacga tccagcccag gtcgccggtt cgcaggtcgt
     1981 tgcgcagcgt catcccgtca actgccattg gatctccttt cgtcatgtga tcaggatcag
     2041 cagccagtag acgaccatcc cgccggcgat gcaggcccag gtccggcgcg tcagccgcga
     2101 gatgacgacc gccagcgccg ccgcgacgat gccc
//
LOCUS       DASUGJ010000149         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1965252, whole genome shotgun sequence.
ACCESSION   DASUGJ010000149 DASUGJ010000000
VERSION     DASUGJ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965252"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..333)
                     /locus_tag="VFV93_03270"
     tRNA            complement(237..333)
                     /locus_tag="VFV93_03270"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(297..299),aa:Sec,seq:tca)
     gene            453..>2398
                     /locus_tag="VFV93_03275"
     CDS             453..>2398
                     /locus_tag="VFV93_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5164394.1"
                     /translation="MAQSPRQLDRARIAQNRRTRASDQRSEISARHIRLLSTLQDVLS
                     AIAGKPDIESELQPLVASISSRFRYANVAIGIIEGEEIVFRGATSSDRRGDFRLPITQ
                     GICGRVARSGVAELVTDLAADPDYVGVINDSTCEACVPIHAGNTVWGVLNVEAARDVT
                     LEAEEFEILMLLAASLGQAIERSRRQRAGLQQHDQLAHLQRLAGRIAGRVDAEQGAED
                     VLTEIRRVFGYSALGLGVVRDGKFDVYHSYAALLDGSQPLRTIPMTGVSGKVARTGEP
                     VFARNVHDEPEYVNTRPDTTQEICVPVRSGDMVVGVLNVEATDERTLDNNDLDLLLTL
                     GDHLGSAISNHLRIAELERRNQQLRLVDRVTSLIAGQITIREALPHVLAEIEGAFGYG
                     SSGIGLIDGDRLVFAAVRDSHEDNIHDYFLDHGVSIETGVTGLVARTGQPVFIKDVSA
                     SPDYLPTSPVIQQEICVPISAHGQVIGVLNVETPSSKPLEQSDFEILTTIANHLGMAF
                     ERSEAYEAERRSRTAMEAIQRVATIVSSTLDSDEALRRIVEMLATVFGYSYVSISLID
                     GEQLDSTAWHGLPDGSVPPPFVVGAGVAGRVAQTGIAELVDLFTADEESSIARADTTS
                     QIVVPIRCSGELAGVISVEGTEQQP"
BASE COUNT          436 a          797 c          744 g          421 t
ORIGIN      
        1 gctgatccag ccagcaggag atacccgccg atcgtaactc agctacgatc ggcaagacgc
       61 ggtctctgtc cgtgcttgca tacgagatga agacgtacgg cgaacctact cgatcgctca
      121 cagacatgat cttgtccctg atattgatga ccggcgtggt gacattgtaa gcgatcatgt
      181 ccgctgtgaa agcatatgtg aggaatactg gaggtccgtt tcggacttcg caagaatggc
      241 gagggcgcat gggagtcgaa cccacctggg aggctcgtca ccccccacaa cggttttgaa
      301 gaccgcacga gccaccgggc ccgatccgcc cccacgttgg gtatggcgtg aaggatagca
      361 tcctgctggt tgttcaggcg gctcatgcta tcgccgttgc gcgtatacta cggcgagaca
      421 cgcgacagga catcgtcagg aaagagggcc gagtggcgca atctccgcgt cagcttgatc
      481 gagcccggat cgcgcagaat cgccgtaccc gtgcatcgga ccagcgctcc gagatcagcg
      541 cgcgccatat tcgcctgctc tcgaccctcc aggacgtcct ctccgccatc gccggcaagc
      601 cggacatcga gtctgagctg cagccgctgg ttgccagtat ctcaagccgc ttccgctacg
      661 cgaacgtcgc gatcggcatc atcgagggtg aagaaatcgt cttccgcggc gctacctcca
      721 gcgatcggcg cggcgatttc cggctgccga tcacgcaggg gatctgtggg cgggttgccc
      781 ggagtggcgt ggccgagctc gtcaccgatc tcgctgccga tcctgactac gtcggcgtca
      841 tcaacgactc cacctgcgag gcgtgtgtgc cgatccacgc tggtaacacc gtctggggtg
      901 tcctcaacgt cgaggcggcc agggatgtca cgctcgaagc cgaagagttc gagatcctga
      961 tgctgctggc cgcctcgctc ggccaggcga tcgaacgctc ccgccggcag cgagccggtc
     1021 tacagcagca cgaccagctc gcacacctgc aacggctcgc cggccggatc gccggtcgtg
     1081 tcgatgccga acaaggcgcg gaagatgtcc tgacggagat caggcgcgtc ttcggctact
     1141 cggcgcttgg acttggcgtc gtccgcgacg gcaaattcga tgtctaccac tcgtacgcag
     1201 ccttgctgga cggctcccag ccgctccgga cgatcccgat gaccggcgtc tcgggcaagg
     1261 tcgcacgcac cggcgagccg gtcttcgctc gcaacgtcca cgacgagccc gagtacgtca
     1321 acacgcggcc cgacacgacc caggagatct gcgtcccggt ccgttccggt gacatggttg
     1381 tcggtgtcct gaacgtcgag gcgaccgatg agcgaacgct ggataacaac gacctcgatc
     1441 tgctgctgac gcttggcgac cacctcggct cggccatctc gaaccacctg cgcatcgccg
     1501 agctggagcg gcgcaaccag cagctgcgac tcgtcgatcg cgtcacctcg ttgatcgccg
     1561 gccagatcac gatccgcgag gcattgccgc acgtcctggc cgagatcgag ggcgccttcg
     1621 gctatggaag ttccggcatc ggactgatcg acggcgaccg gctcgtcttc gcggccgtca
     1681 gagactccca cgaggacaac atccacgact acttcctcga ccacggcgtc tcgatcgaga
     1741 cgggcgtgac tggtctcgtc gcccggaccg gccagccggt cttcatcaag gatgtcagcg
     1801 cctcgcccga ctacctgccg accagcccgg tcatccagca ggagatctgt gttccgatca
     1861 gcgcgcacgg gcaggtgatc ggcgtgctga atgtcgagac gccatccagc aagccgctgg
     1921 agcagagcga cttcgagatc ctgacgacga tcgccaacca cctcgggatg gcattcgagc
     1981 gcagcgaggc ctacgaggcg gagcggcgca gtcgaacggc gatggaagcc atccagcgcg
     2041 tcgcgacgat cgtctccagc accctcgatt ctgacgaagc cctgcgccgg atcgttgaga
     2101 tgctggcgac ggtcttcggc tattcgtatg tgtcgatcag cctgattgat ggcgaacaac
     2161 tcgattcgac cgcctggcat ggcctgcccg atggcagtgt cccgccgccg ttcgtggtcg
     2221 gcgctggtgt ggccggccgc gtcgcccaga ccggcatcgc tgagctggtg gatctcttca
     2281 ccgccgacga ggagtccagc atcgcccgcg cagacacgac ctcccagatc gtcgtcccga
     2341 ttcgctgttc cggcgagctg gccggcgtca tcagcgtcga gggcaccgag cagcaacc
//
LOCUS       DASUGJ010000150         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1130865, whole genome shotgun sequence.
ACCESSION   DASUGJ010000150 DASUGJ010000000
VERSION     DASUGJ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130865"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..849)
                     /locus_tag="VFV93_03280"
     CDS             complement(40..849)
                     /locus_tag="VFV93_03280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5164395.1"
                     /translation="MGMRFTGERVIPEMPEMRVTFLQSLAAYEFAAQLASGKRVLDCG
                     SGEGYGTALLANDAAFVVGLDRDPETVAYASGKYADAERAAFIVANADRLPFADDSFG
                     LAVCFQVIEHLPDPLAFIREVHRLLAPGGTFVLTTPNLLVTGARPNPHHVHDYAPDDL
                     RGLLERVFADVELRGVFAGEHVAAYRARNDRIVRTIMRLDPLGLHRRIPSRLVESVHI
                     RVTRLIRGRLNASNPDLVSSLTTADFPIGSEQIEHAIDLLAACRKRAGLSS"
     gene            complement(849..1670)
                     /locus_tag="VFV93_03285"
     CDS             complement(849..1670)
                     /locus_tag="VFV93_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5164396.1"
                     /translation="MVVERSRRSCLLCGRSFEGRTFLCRDCSDAWRGRPIPAEVRARF
                     YEALDRAYPDRSNTYGNWNVPVMLLREIERLPRELRVLEIGAGGGFFAVELGRRGFSD
                     LTLSDFTATTLAALRERLPEARLVGADGAQLPFRDGSFDVVISTDVIEHIPEADQHIA
                     EVARILAPSGRYYVKTPNRPLAEAFYRLRGMHDAYFWHPSMFSAGELRAAFGRHGLAT
                     RMLAQPRLTGAQLAKLPGPRRLRRLAGALPLGWLPAGVLPHLEAVGAKRAVKEGD"
     gene            complement(1673..2422)
                     /locus_tag="VFV93_03290"
     CDS             complement(1673..2422)
                     /locus_tag="VFV93_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164397.1"
                     /translation="MVLALALLTTACTTRVTGYSSPIVHDYAVVEWHGLGQVVEQRFV
                     AGPGHQAAIANDGKTTPAQFEERLTALPEDARRGALEEMTIGLRLDGEGEEQQFGVWE
                     TGARVQVLGPDGALVFDVAVDGLPREDDWLRIDFPPALVPGQEVTLRIVPGTGDDAGR
                     LQYGVTPDRAPYGGWMATNADGSSAGGALLLRTVYKRDVALGSVVSDGFGNLRDAAGD
                     DPLASALWALAMAGLLGGAGWLWRARPVRGA"
     gene            complement(2466..>3577)
                     /locus_tag="VFV93_03295"
     CDS             complement(2466..>3577)
                     /locus_tag="VFV93_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164398.1"
                     /translation="GFTSQFLIQSFHNLWLLIIRPLLPDSHAIWSESASTLGVGASRA
                     DYERFLAYDHLYRAIFLMKLPYVVADLGCAWTLTKLVPAGKRVAVAAFWLLNPLVIFS
                     SAIYGRHDSIAILLVLLSVVAARRATDDGRLVGLALLGIATLARFFPAIIVPLFLMAF
                     RRTARQLALFLGLLLGLVGLVELAGLATSGKSTILTILNSYEHFQYWFDAGLYLRFDD
                     WIFLFPAVYIVALLWISERGITPEEYLPFSAAAFLLLFSLTFFHPHYAVWLVPFLALT
                     IPLAADSKRLTVYHAIQIVCILIYSAQWGAWTTWDLLEPLLGDRVASLPDPVEAIAGQ
                     IEPRLFVGLFRSILTAVSLWMAWRILRPVVRGERG"
BASE COUNT          641 a         1270 c         1185 g          481 t
ORIGIN      
        1 gccatacgaa gctgacgcag ggaggtcctt cgctgcggct caggacgaca gtcccgcgcg
       61 cttccggcag gccgccagca agtcgatggc gtgctcgatc tgctcgctgc cgatcggaaa
      121 gtcggccgtc gtcaggcttg ataccaaatc cggattgctg gcgttgagcc ggccacgaat
      181 cagccgcgtc accctgatgt ggacggactc gaccagccgg ctgggaatcc gccggtggag
      241 gccgagcggg tcgagccgca tgatcgttcg gacgattcgg tcgttgcggg cgcggtaggc
      301 ggccacatgc tcgccggcga agacaccgcg caattcaaca tcggcgaaga cacgttccag
      361 caatccgcgc aggtcgtccg gcgcgtagtc gtggacgtgg tgcgggttcg gccgcgcgcc
      421 ggtcaccagc aggttcggcg tcgtcagcac gaacgtcccg cccggcgcca gtagccggtg
      481 cacctcgcgg atgaacgcca gcgggtcggg cagatgctcg atcacctgga agcagacggc
      541 cagtccgaag ctgtcgtccg cgaacggtaa ccggtcggcg ttggcgacga tgaaggcggc
      601 ccgttcggca tccgcatact tcccggacgc gtaggcgacc gtctccggat cgcggtcgag
      661 gccaacgaca aaggcggcat cgtttgccag cagcgccgtc ccgtacccct cgccgctgcc
      721 gcagtcgagg acgcgttttc cggaggccag ttgcgcggcg aactcgtagg ccgccaggct
      781 ctggaggaac gtgacgcgca tctcgggcat ctccgggatg acccgttcgc cggtgaagcg
      841 catgcccatc agtcgccctc cttcactgcc cgtttcgcgc cgacggcttc gaggtgcggc
      901 agtacacccg ccggcagcca gccgagcggc agcgcgccag ccagccgccg caaccggcgc
      961 ggccccggca gcttggcgag ctgggcgcca gtcaagcgcg gctgggccag catccgcgtc
     1021 gcgaggccgt gccgcccgaa ggcggcccgc aactccccgg ccgagaacat cgacggatgc
     1081 cagaagtagg cgtcgtgcat cccgcgcagc cggtagaacg cctcggccaa cggccggttc
     1141 ggcgtcttga cgtaatagcg accactcggc gcgagaatcc ttgcgacttc ggcgatgtgc
     1201 tgatcggcct cgggaatatg ctcgatcacg tcggtcgaga tgacgacatc gaagctgccg
     1261 tcgcgaaagg gcagctgcgc gccgtccgcc ccgaccagcc gggcctccgg cagccgctca
     1321 cgcagtgccg ccagcgtcgt cgccgtgaag tcggagagag tcagatcgct gaagccgcgc
     1381 cggcccaatt cgaccgcgaa aaagccgcca cctgccccga tctccagcac acgcagctcg
     1441 cgcggcaggc gctcgatctc ccgcagcagc atgaccggca cgttccagtt gccgtaggtg
     1501 ttggagcggt ccgggtaagc acgatccagc gcctcgtaga agcgggcgcg gacctcagcc
     1561 gggatcggcc tcccacgcca cgcgtccgag cagtcgcgac agaggaacgt ccgcccctcg
     1621 aagctcctgc cgcatagcag gcacgatcgc cgcgaccgct cgacgaccac cgctaggctc
     1681 cccgcaccgg ccgtgcccgc cagagccaac cggccccgcc cagcaacccg gccatcgcca
     1741 gcgcccagag cgcactcgcc agcggatcgt caccagcggc atcccgcagg ttgccgaagc
     1801 cgtcgctcac gaccgagccg agcgccacat cccgcttgta gacggtccgc agcagcaacg
     1861 ccccaccggc actgctgccg tccgcgttgg tcgccatcca gccgccatag ggcgctcgat
     1921 ccggcgtgac gccgtactgc agccgccccg cgtcatcgcc agtccccggc acgatccgca
     1981 acgtcacctc ctgccccggc acgagcgccg gcgggaagtc gatccgaagc cagtcgtctt
     2041 cgcgcggcag gccgtcgacc gccacgtcaa agacgagcgc gccatccggt cccaacacct
     2101 gcacccgcgc gcccgtctcc cagacgccga actgctgctc ctctccctcg ccgtccagcc
     2161 gcagcccgat cgtcatctcc tccagcgcgc cgcgccgtgc gtcctccggc agggccgtca
     2221 gccgctcctc gaactgcgcc ggcgtcgtct tcccatcgtt ggcgatcgcc gcctgatggc
     2281 ccggccccgc cacgaagcgc tgctcgacca cctgccccag cccatgccac tcgacaaccg
     2341 cgtaatcatg gacgatcggg ctggagtagc cggtgacacg ggttgtgcag gcagtggtga
     2401 ggagggcgag ggcgagcacc atatggggga aaaccccctc cccccatctg gcgcgcaaag
     2461 gcaccctagc cacgctcccc cctcacgacc ggccgcagaa tccgccacgc catccagagt
     2521 gatactgccg tcaggatcga gcgaaagagg ccgacgaaga ggcgcggctc gatctggccg
     2581 gcgatcgcct cgaccgggtc ggggagcgaa gcgacccggt caccgagcag cggttcgagc
     2641 aggtcccagg tcgtccaggc gccccattgg gccgagtaga tcaggatgca gacgatctgg
     2701 atcgcgtggt agaccgtcag ccgcttacta tcggctgcca gcgggatcgt cagcgccagg
     2761 aacggcacca gccagacggc gtagtggggg tggaagaacg tcaggctgaa caacaacagg
     2821 aaggcggctg cgctgaatgg caggtattcc tcgggcgtga tgccccgctc gctgatccag
     2881 agcagcgcga cgatgtagac ggccgggaag aggaagatcc agtcatcgaa gcgcaggtag
     2941 aggccggcgt cgaaccagta ctggaagtgc tcgtaggagt tgaggatcgt caggatcgtg
     3001 ctcttgccgg aggtcgccag tccggccagc tcgaccagcc cgaccagccc cagcagcagc
     3061 ccgaggaaga gcgccagctg gcgggccgtc cgccgaaacg ccatcaaaaa gagcgggacg
     3121 atgatcgccg ggaagaagcg tgccagcgtc gcaatcccca gcagcgccag cccgaccagc
     3181 cgtccgtcgt cggtcgcccg gcgagcggcc acgaccgaga gcagcaccag caggatcgcg
     3241 atgctgtcgt gccgcccgta gatggccgac gagaagatca cgagcgggtt gagcagccag
     3301 aaagcggcca ctgccacccg cttgccggcc gggacgagct tcgtcagcgt ccaggcgcag
     3361 cccaggtcgg cgacgacgta gggcagcttc atcaggaaga tggcgcggta gaggtggtcg
     3421 taggccagga agcgctcgta gtccgcccgg ctcgcgccga cgccgagcgt cgatgctgac
     3481 tccgaccaga tcgcgtggct gtcgggcagg agtggccgga tgatcagcag ccagaggttg
     3541 tggaacgact ggatcaggaa ctggctcgtg aagccga
//
LOCUS       DASUGJ010000151         5259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_19927, whole genome shotgun sequence.
ACCESSION   DASUGJ010000151 DASUGJ010000000
VERSION     DASUGJ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19927"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1980
                     /locus_tag="VFV93_03300"
     CDS             <1..1980
                     /locus_tag="VFV93_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX5164399.1"
                     /translation="PDLEADLQRSISERGEVLDSASEALRKIRQELRSAHARLIERVN
                     RYIQGSQYSAAIQDPIVTQRDGRYVIPVRADRRGQIPGVVHDTSASGQTLFLEPMEVV
                     ALNNRWRELQAAERHEIDRILTALSHRIGDAEPELASTLDALAAIDLALAKARYASAM
                     RATRPAVVDHTSVQGRRIALNKARHPLLDQTTVVPIDVVIGDGFRVLVITGPNTGGKT
                     VALKTIGLMAVMAQSGLFLPADEGSEVCVFDGIFADIGDEQSIEQSLSTFSSHMVRVI
                     SMLRDVTSDSLVLLDEMGAGTDPEEGAALARALIEDLLAKGCLAVATTHYSELKAFAY
                     TTAGTENGSVEFDLATLSPTYRLVVGVPGQSNALAIASRLGMPDRVIDRARSFLSPDV
                     ERVDSLLSEIRQRRDEADAARREASRARAEAERLRASAQEQLAEAESIRQAARQDAIG
                     EVERELADARQALRRVRETPERLPREQLQPTVQESRAELEQAAQEVRQYVRRRPPRTT
                     NVQLKVGDTVEITSLGGEGEVLGFSDDGTEADIQMGAFKVRQPIAMLKRIGGPRSVKQ
                     PRYVAPPPRRDVDMELHLRGQRAAGVDQLVDDYLNDAYLSRLPYVRIVHGKGTGALRE
                     VVRQVVARHPLVDRWETPPQNEGGDGVTVVHLRES"
     gene            2002..2274
                     /locus_tag="VFV93_03305"
     CDS             2002..2274
                     /locus_tag="VFV93_03305"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX5164400.1"
                     /translation="MSDVDLPDGSRVVEIAVAPNELTADMWRQALEDDGIIAALKPGG
                     VGFSFGSSALMEHYILVREDQAERARAIISELESSDEDDESWSSSS"
     gene            complement(2193..>5259)
                     /gene="mfd"
                     /locus_tag="VFV93_03310"
     CDS             complement(2193..>5259)
                     /gene="mfd"
                     /locus_tag="VFV93_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX5164401.1"
                     /translation="AAAGYQVEATVEGPGTLSRRGGILDVYTPGADDAIRIEFFGDEI
                     DSIRRFDPGTQRSIERIGEVTILPPIEFDLSDRSRAVASLREHPTASLREEVRDEWER
                     LLALLEAGEVPASIDLLATAFPSNSATLLDYLPEGSVVARIEPESQALQGSQIELQAE
                     DTRQTLEQAGEIPPGFDRPYQRWTDVEQAFGRFDTWEIGAGSEEGIDLPASAAFAPIP
                     AFGGEIDDLANYLRATTGEGGRVVVATDQSERLRDLLEEHEIYPRVLKRGGSVSGPPP
                     AGAVDVVHAPLGAGFALNGVQPLVVLSDLEVFGLRKVVRPAAPQQRRRMTPIKQFVPG
                     SYVVHVEHGVGEFKGLVTLDLSGAEREYLQVDYADGDRLYVPVDQADRLMPYESPAGT
                     PRITRLSSPEWSRIKSRVRKAVREMAFELIQIYAAREVAQGHPFPADSSWDIELAESF
                     PFRETADQQKAIDDVKGDMETPRPMDRLVCGDVGYGKTEVALRAAFKAINDGMQVAVL
                     VPTTILALQHYNTFRERLAAFPVKVEMLSRLRTKPEQTAITEGLASGAIDIVVGTHRL
                     LQRDVNFKNLGLLVVDEEQRFGVSHKEHIKRARAEVDVLTMTATPIPRTLHMALTGVR
                     DLSLISTPPQDRVPIRTFVTPTSDAIVRESILREVARGGQVYVVHNRVQSIYRLSDHL
                     KELVPEASFAVAHGQMDEKDLERIVLAFIRHEFDVLICTTIIESGVDIPNANTIILDN
                     AHALGLTQMYQLRGRVGRGTNRAYAYLLYPPNTPLSSEALERLEAIQEATELGAGFQV
                     ALRDMEIRGAGNILGAEQSGHIAAVGFDLYTRMLAHAVEEVRAGHPIAEPEEVSLDIA
                     VDAGIPEEYISDEQVRLDTYRRIAAASNERALRDLDAELVDRFGPVPAAVVRLFDLVR
                     LRQRASRLGLVGIIERDGDIVIRPVIGSRLDQQQLRRQLGVGVRVTPNQVRLRVADLR
                     VDRWDAIVAVLDAVSARRATSVAQDEDDQDSSSSSEDSSSEMMARARSA"
BASE COUNT          939 a         1692 c         1753 g          875 t
ORIGIN      
        1 cccgacctcg aagcggacct gcaacggtcc attagcgagc gcggcgaggt gctcgacagc
       61 gcgagtgagg cgttgcgcaa gatccggcag gagctccgct cggctcacgc acggctgatc
      121 gaacgagtca accgctatat acaaggatcg caatactcgg ccgccatcca ggatccaatc
      181 gtgacacagc gggatggccg ctatgtcatc ccggttcgcg ctgaccggcg cgggcagatc
      241 cccggcgtcg ttcacgatac gtccgccagt ggacagacct tgttcctgga gccgatggag
      301 gtcgtcgcac tcaacaaccg ctggcgggag ctacaggccg ccgagcgaca cgagatcgat
      361 cggattctga cggcgctctc acaccgcatc ggcgacgccg aaccggagct ggccagcacg
      421 ctcgacgcac tggccgcgat tgatctcgca ctggcgaagg ctcggtacgc ctcagccatg
      481 cgagcgacac ggccggctgt cgtcgatcac acgtcggtgc agggccggcg tatcgcgctc
      541 aacaaggccc ggcacccgtt gctggatcag acgacggttg tcccgatcga tgtcgtgatc
      601 ggtgacggct tccgcgtgtt ggtcatcacc ggtccgaata cgggcggcaa gacagtcgcg
      661 ctcaagacga tcggtctgat ggcggtcatg gcacagtcgg gcctgttcct tcctgccgat
      721 gagggtagtg aggtctgcgt cttcgacggc atcttcgccg acatcggcga tgagcaaagc
      781 attgagcaaa gcctgtcgac gttctcctcg cacatggttc gcgtcatctc gatgctgcgt
      841 gacgtgacca gcgacagcct cgttctgctg gacgagatgg gcgccggaac cgacccggag
      901 gaaggggccg cgctggctcg ggcgctgatc gaagatctgc tggcgaaggg ctgtctcgcg
      961 gtcgcgacga cacactattc cgaattgaag gcattcgcat acacgacggc cgggaccgag
     1021 aacggcagcg tcgagttcga tctggcgacc ctgtctccga cctatcgtct ggtcgtcggc
     1081 gttccagggc aatcgaatgc gctggcgatc gcgagccggt tggggatgcc ggatcgcgtg
     1141 atcgaccggg cacgctcgtt cctcagcccg gatgtcgagc gggtcgactc gctgctcagc
     1201 gaaatccggc agcgccgcga cgaggccgac gcggcccgtc gcgaagcgtc acgggcacga
     1261 gccgaggccg agcgcctgcg tgcctcggca caagagcagc tggccgaggc ggaatcaatc
     1321 cggcaggcag cccggcagga cgcgatcggt gaagttgagc gcgagctcgc tgacgcccgc
     1381 caggcgctcc ggcgcgtcag ggaaacgccg gaacgtctcc cgcgcgagca gttgcagcca
     1441 acggtccagg aatcgcgggc ggagcttgaa caggcagcgc aagaggttcg ccagtatgtc
     1501 cggcgacggc cgccgcgaac gacgaacgtc cagttgaagg ttggcgacac cgttgagatc
     1561 acgtcgctgg gcggcgaggg cgaggtgctc ggcttctctg atgatggaac cgaggctgac
     1621 attcagatgg gggcgttcaa ggttcgccag ccgatcgcga tgctgaagcg catcggaggc
     1681 ccgcgcagtg tcaagcaacc gcgatatgtc gccccgccgc cccgccgcga tgtcgacatg
     1741 gagctgcacc tgcgtgggca acgggcggcc ggcgtcgacc aactggttga cgactacttg
     1801 aacgacgcct acctttcacg cttaccctac gtgcggattg tgcacggcaa aggtaccggg
     1861 gcgttgcgcg aggtcgttcg tcaggtcgtc gccaggcacc cactcgttga ccgctgggag
     1921 accccaccac agaacgaggg cggcgacgga gtcaccgtcg tacatctcag ggaatcctga
     1981 ggaaagatga ttgagggaat catgagtgac gttgatctgc cggacggaag cagggtcgtt
     2041 gagattgccg tcgcgccaaa cgaactgacg gccgatatgt ggcgacaggc gcttgaagac
     2101 gacggcatca ttgcggcgct caagccgggc ggagtcggct tctctttcgg atcgagcgcg
     2161 ctgatggagc attacattct ggtgcgtgag gatcaggccg agcgggcgcg agccatcatc
     2221 tccgaactgg aatcctctga cgaggacgat gaatcctggt cgtcctcgtc ctgagcgacg
     2281 ctggttgccc gtcgcgctga gacggcatcc agaacggcga cgatcgcatc ccagcgatcg
     2341 actcgcaggt cggccacacg caggcgtacc tgattcggcg tgacccgcac gccgacaccg
     2401 agctgacgac gcagttgctg ttggtcgagc cgcgagccga tcacgggccg gatgacgatg
     2461 tctccgtcgc gctcgatgat gccaaccagc ccgagccggc tggcacgctg ccgcaaccgg
     2521 accagatcga acaggcgaac gacggcagcc gggaccggcc caaagcggtc gacgagttca
     2581 gcgtcaaggt cgcgcagggc gcgctcgttg gaagcagccg cgattctccg atacgtgtcg
     2641 agccggacct gctcgtcgct gatgtactcc tcggggatgc cggcatcgac ggcgatgtcg
     2701 agcgatacct cttccggctc agcgatcgga tggccggcac gaacctcctc aacggcatgc
     2761 gcgagcatgc gggtgtagag gtcgaaaccg acggcagcga tatggccgga ctgctccgcg
     2821 ccgaggatat tgccggcgcc acgaatctcc atgtcgcgca gggcgacctg gaagcccgcg
     2881 ccaagttcgg tcgcttcctg gatcgcctcc agccgctcca gcgcctcgct ggagagggga
     2941 gtattcggcg gatagagcag gtaggcgtag gcgcgattgg ttccacgtcc gacccgcccg
     3001 cgcaactggt acatctgcgt cagtcccagc gcgtgggcgt tgtcgagaat gatcgtgttg
     3061 gcgttcggaa tatcgacgcc cgactcgata atcgtcgtgc agatcagtac gtcgaactcg
     3121 tgacggatga aggccaacac gatccgttcc agatccttct cgtccatctg tccgtgggcg
     3181 acggcgaagc ttgcctccgg taccagctcc ttgaggtgat cggagaggcg gtagatactc
     3241 tggacgcggt tgtgaacgac gtaaacctga ccgccacgag caacctcgcg caggatcgac
     3301 tcacggacga tcgcgtccga cgtcggtgtc acgaaggtgc ggatcggcac tcgatcctgc
     3361 ggcggcgtcg agatcagtga gagatcacgg acgccggtca gcgccatgtg caacgtgcgc
     3421 ggaattggcg tcgccgtcat cgtcaggaca tcgacctcag cccgggcgcg cttgatgtgc
     3481 tctttgtgag agacgccgaa gcgctgctcc tcgtcgacga ccagcaggcc gaggttcttg
     3541 aaattcacat cccgctgcaa cagccggtgg gtgccgacga cgatgtcgat cgcgccggag
     3601 gccagtccct cggtgatggc ggtctgctcc ggcttcgtcc gcaggcgcga gagcatctcg
     3661 accttgaccg ggaaggctgc cagccgttcc cggaaggtgt tgtagtgctg gagcgcgagg
     3721 atcgtcgtcg ggacgaggac ggcgacctgc atgccgtcgt tgatcgcctt gaacgcggcc
     3781 cgcagggcaa cctcggtctt gccgtagccg acatcgccgc agaccagccg gtccatcggt
     3841 ctcggcgtct ccatgtcgcc cttgacgtcg tcgatcgcct tctgctggtc agccgtctcg
     3901 cggaacggga acgattcggc cagctcgatg tcccacgacg aatcggccgg gaacgggtgg
     3961 ccctgggcga cctcgcgggc cgcgtagatc tggatcagct caaacgccat ctcacggacg
     4021 gccttgcgga cccgcgactt gatccgtgac cactccggcg acgagagccg cgtgatgcgc
     4081 ggcgtcccgg ccgggctctc atacggcatc agccggtcgg cctgatcgac gggaacgtaa
     4141 agccggtcgc cgtcggcgta gtcgacctgc aggtactcgc gttcagcccc agacaggtcg
     4201 agcgtcacca gtcctttgaa ctcgccgaca ccgtgctcga catgaacgac atacgagccg
     4261 ggcacgaact gcttgatcgg cgtcatccga cggcgctgct gcggggcggc aggacggacg
     4321 accttgcgca gcccgaacac ttccaggtcc gagaggacga ctaacggttg gacaccgttt
     4381 agcgcgaagc cggctcccag cggtgcgtgg accacatcga ccgcgccggc cggaggcggg
     4441 ccgctgaccg agccgccacg cttgagcacc cgcgggtaga tctcgtgttc ctccagcagg
     4501 tcgcgcaagc gctcgctctg atcggtcgcg acgacaacgc gtccgccctc gccggtcgtc
     4561 gcacgcaggt agttcgccag atcgtcgatc tctccgccga aggccgggat cggcgcgaac
     4621 gccgcgctgg ccggcagatc gatgccctcc tcgctgccgg caccgatctc ccaggtatcg
     4681 aaccggccga acgcctgctc gacatccgtc cagcgctggt agggccggtc aaagcccggc
     4741 ggaatctcgc ccgcctgctc aagcgtctgg cgggtgtcct cggcctgaag ctcgatctgc
     4801 gatccctgga gtgcctgtga ctccggctcg atccgggcga cgacactccc ttcgggaaga
     4861 taatcgagca gggtcgcgct attgctgggg aaagccgtcg ccagcaggtc gatcgacgcc
     4921 ggcacctcgc ccgcttcaag cagcgccagt aagcgttccc actcgtcccg aacctcctcg
     4981 cgcaagctgg cggtcggatg ctcgcgcagg ctggcgacgg cacgtgagcg gtccgacagg
     5041 tcgaactcga tcggcggcag gatcgtgacc tcgccgatgc gttcgatcga gcgctgcgtg
     5101 cccggatcga aacgtcggat gctgtcgatc tcgtcgccga agaactcgat ccggatcgcg
     5161 tcatcggcgc cgggagtgta gacatcgaga atgccgccgc gccgactgag ggtgcccggt
     5221 ccctcgacgg ttgcctcgac ctggtagccg gccgcggcg
//
LOCUS       DASUGJ010000152         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_297927, whole genome shotgun sequence.
ACCESSION   DASUGJ010000152 DASUGJ010000000
VERSION     DASUGJ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297927"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..579
                     /locus_tag="VFV93_03315"
     CDS             79..579
                     /locus_tag="VFV93_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5164402.1"
                     /translation="MSIQRWDPLRDIVSLREAMNSLLEESFVRPRAGMVAMTGGMAID
                     LRENDQAYIVESVVAGAKPEDVEISVLGDTLKIAVEVREDADRHDEKWLVRERRFGRF
                     ERTLTLPSQVKADEASADFHEGILTVTLPKADIARPKTIPVRGATTGQQRAEPQEIDV
                     MSEPGE"
     gene            917..1147
                     /locus_tag="VFV93_03320"
     CDS             917..1147
                     /locus_tag="VFV93_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164403.1"
                     /translation="MDIWEIASVIRAFAGTSDTWVDQVTALTSTHPTNVRIAHRYYVE
                     YRDEIDEWIRRNNEHAERAEDAWRRERGLTDP"
     gene            1273..>2678
                     /locus_tag="VFV93_03325"
     CDS             1273..>2678
                     /locus_tag="VFV93_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEX5164404.1"
                     /translation="MSRDEMIERMLASDPADDGAFIVGVRTTGIYCLPSCTPPRKPKP
                     ENVEFYATPEEARAAGLRACKRCRPDDFYLGQHAGEALVEGLVAGVALDPGAYRNLSA
                     LTAAADVGSRKLHDLFRTHYHSTPAEMLARLRVAAARRMLLHGQRPVAEIAFEVGFES
                     LSAFNENFRKYTAMSPARYRQLPDETTFDLSLPDDYPLGRILAYLGRDKNSLTERVEG
                     QTCLIAYWAGEQPALVRIVFEDGIARCTVLLPEQPNPDLMEALHSRVLATLGLTVDPA
                     RFEALVTGSPELAPLIDGQRGLRVPLLADPFDGLVWAIVGQQINLAFAYTLRRRLIEH
                     VSQPLGEGLYAPPSAVAVAAIEPEDLLPLSFSRAKASYLVGAARSVIDGRLPLDALAG
                     SSATRIERTLLAVRGIGPWSAHYLMMRHYGFLDCVPLGDTGLTSGLKQFFALDERPGK
                     LETLALMDRFSPYRSLAT"
BASE COUNT          473 a          824 c          902 g          479 t
ORIGIN      
        1 agacatcggg cgtgtcgagt tcagcaccgg ccacgctcca ccgacacgac agcagagcat
       61 tcagggagga ttgacagcat gagtattcaa cgttgggacc cactacgcga cattgtctcg
      121 ctgcgtgagg cgatgaacag cctgcttgag gagtcgttcg tccggccgag ggccggaatg
      181 gtggcgatga ccggcgggat ggcgatcgac ctgcgcgaga acgaccaggc ctacatcgtc
      241 gagtcggtcg tggccggcgc gaagccggag gatgtcgaga tctcggtcct gggcgacacg
      301 ttgaagatcg ccgtcgaggt tcgcgaggat gccgaccggc acgacgagaa gtggctggtg
      361 cgcgagcgcc gcttcggccg cttcgagcgg acactgacgt tgccgagcca ggtcaaggcc
      421 gacgaggcca gcgccgactt ccacgagggt atcctgacgg tgacattgcc gaaggccgac
      481 atcgccaggc cgaagacgat tccggtccgc ggcgcaacaa ccggccagca gcgcgccgaa
      541 ccacaggaga tcgacgtcat gtcggagccg ggcgagtagc ggctgccagc gaacgaccgt
      601 ccgaacacag ccagattttc ggggtgcctt atgtggcacc ccgtttcttg tgcaggcaac
      661 tgccatatca atcaggatgc tacagtgtga agaggactgc aggtctgcgg aaggaatcat
      721 cgcggcaagc catcaccgat ccatacatgt caataagaag attctggaac gcttggatgc
      781 agaaagtggc cagcttggaa tcactcgctc ggaccttgca acgacgctgc ttgaagaggg
      841 cttgcgaatg ctcgctcatc ccggcattgt ctttcggccc gggccagcgg ggcgtcgccc
      901 aggtttggcc tgtggcatgg atatctggga aattgccagc gtcatacgtg cattcgccgg
      961 cacgagcgat acatgggtcg atcaggtcac cgcattgact tcaactcacc ccacgaatgt
     1021 tcgcatcgct catcggtatt acgtcgagta tcgcgatgag atcgatgagt ggattcgtcg
     1081 caacaacgaa cacgctgagc gtgctgaaga cgcttggcga cgtgagcgtg gactcactga
     1141 tccgtgagcg gcccttcttg atgtgtagtg ttcagtcagt gagagaccgt ggaattgaaa
     1201 cgcaggattc aggtagaaca tctgtccgta tcctgctatt ctgggtgcat gaagactgcg
     1261 acgcgagtga cgttgagccg ggatgagatg atcgagcgga tgctggcgag cgacccggct
     1321 gatgatgggg cgtttatcgt cggcgtccgg acgaccggga tctattgcct gccatcctgt
     1381 accccacccc gcaagccgaa gccggagaat gttgagttct atgcgacgcc cgaggaggcg
     1441 cgggcggccg ggctgcgggc ctgcaagcgc tgccggccgg acgatttcta cctcggtcag
     1501 catgctggcg aggcgctggt cgaggggctg gtggctggcg tcgcgctcga ccccggcgcc
     1561 taccgcaacc tgagcgcgct gacggccgcc gctgatgtcg gcagtcgcaa gctgcacgac
     1621 ctgtttcgca cccactacca ctcgactccg gccgagatgc tggcccgcct gcgggtcgcg
     1681 gcggcgcggc ggatgctgct gcacggccag cggccggttg ccgagattgc cttcgaggtt
     1741 ggcttcgaga gcctgtcggc cttcaacgag aacttccgca agtacaccgc gatgagcccg
     1801 gcccgctatc gacaactgcc ggatgagacc acgttcgatc tgagcctgcc ggatgattat
     1861 ccgctcggca ggatactcgc ctacctcggg cgggacaaga acagcctgac cgagcgggtc
     1921 gaggggcaaa cctgcctgat cgcctactgg gccggtgagc agccggcgct ggtgcggatc
     1981 gtcttcgagg acggcattgc gcgctgcacg gttcttcttc ccgagcagcc caaccctgat
     2041 ctgatggagg cgctacacag ccgggtgctg gcgacgctgg ggctgacggt tgacccggcc
     2101 cgcttcgagg cgctggtgac cggctcgccg gaactggctc cgctgatcga cggtcagcgc
     2161 gggctgcggg tgccgctgct ggcggacccg ttcgacgggc tggtctgggc gatcgttggc
     2221 cagcagatca acctcgcctt cgcctacacg ctgcggcgcc gcctgatcga gcacgtcagc
     2281 cagccgctcg gcgaggggct ctacgcgccg ccgtcggcag tggccgtcgc cgcgatcgag
     2341 ccggaggatc tgctgccgct gagcttttca cgcgccaagg ccagctacct ggtcggcgct
     2401 gcccgctcgg tgatcgacgg gcggctaccg ctcgacgcgc tggccggtag ctcggcgacg
     2461 cggatcgagc ggacgctgct ggccgtccgg gggatcggcc cgtggtcggc ccattacctg
     2521 atgatgcgtc actatggctt tctcgactgc gtgccgttgg gcgacaccgg gctgacgagc
     2581 ggcctcaagc agttcttcgc tctcgacgag cgccccggca agctggagac cttggcgctg
     2641 atggaccgct tcagccccta ccgcagcctg gcgacctt
//
LOCUS       DASUGJ010000153         4687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_715030, whole genome shotgun sequence.
ACCESSION   DASUGJ010000153 DASUGJ010000000
VERSION     DASUGJ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715030"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..2456)
                     /locus_tag="VFV93_03330"
     CDS             complement(243..2456)
                     /locus_tag="VFV93_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX5164405.1"
                     /translation="MVGTIEKPDKRAFKRRVKQAVADESLRTALGRALPEFGRRRINA
                     FADQDFAARRRRVRDLKAGAIEHLPELVERFTKEAEAVGAVVHIASTPEDAQRIITEI
                     ATQRAAKLVVKSKSMVSEEIQLNPALEAAGLQVVETDLGEWIMQLAHEHPSHLIAPAI
                     HKTREQVAALFSKEVGVELPADPVELVKVAREKLRQAFIDADIGISGANVAIADSGTL
                     VIVSNEGNGRLVTTLPPVHIALLGIEKIVPSLEDATAILQVLPRSGTGQKITSYVSFV
                     TGPSRSADIELTLTVGVHGPKELHIVLLDNGRWATREDPDLHEMLHCIRCGACSNVCP
                     PYQIVGGHQFGHIYTGPIGLVMTAAHHGLAAAAGPQSLCVSCNACETVCPAEIPIPRM
                     ILDVRSRVVEKFGLPISKSVGLSQWSKPGVGGFAAGIAARASSFVADGERVIRTIPGM
                     GSLTDGRALTAPATDPLRDRVRRNGALTSETTILPSSKARGMQVAFFPGCLTDRLLPE
                     MGEAVITVLRACGCDVQFPTDQHCCGLVAFNSGDRSHGRVMAEQTIRMLETVEADWVL
                     TNSTSCLAAIVQDYQHIFRDDSEWRERAAIQGKRLIDFTTFVDEVAQLDPADFASGNP
                     GPTVTIHDACQSANALGLGAGARRIVAGVLGLELREMQDSRVCCGFGGSFSVDYPQVS
                     TAILGKKLVNAEDTAADVVISDNPGCIMQLRGGLIARNSSTRVLHLAELIAERLP"
     gene            2663..>4687
                     /locus_tag="VFV93_03335"
     CDS             2663..>4687
                     /locus_tag="VFV93_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX5164406.1"
                     /translation="MESDSHPIQQHRDAFAATWRDLVREISPTYREMGQARLRDRIQE
                     GIDALVAPGDVENEASRHFLNNLESIIDQERLSVEEVISGMLASFDCMDLVIPAEQRY
                     SPPMIHSERVLRSMIARFTESAIGIVTQKLEVEASAQRESRARLLSLQRVGAAVTSSL
                     ELESTLETIVQEAAELMNGATARLRLADEVGEHLRLIASAGDIGEDLPGSVVPVETTL
                     AGLCYRSGRPVISNDVAGDPRSDSELQQLTRTRSLLSVPLLSRGTSIGVLSITNLTDR
                     PFVDADAEMLSLFADHAAVAIENGRLFQQAQSQITEMEILNRVSAVVSASLNLSQVYR
                     AIHAEIARIMVADAFLIFLRGPEGGFDLVYIVDLGQTFSPRHDVPLPQVYKQSMEQGV
                     PFIVEASRDQFLGGWERYGDMGRRVQSLMVAPLMRGTEAIGVVTTQSYAPSSYRQRDA
                     DLLATVANVAAVAIENAKLYEQAHGLAVADERNRLAREIHDTIAQGLVGIILQLEALA
                     GMIGDEPRMKRRVDRAIDLARVNLDEARRSVRDLRAAPLEHMSLAEAVRQLAAEHEEE
                     CDSEIVVNVPDAMPLLDKQIETALFRFVQESLTNCRKHALGVPVWVDVEIAECVTISV
                     RDSGPGFDVEAWRSKAPLHHFGLHGMRERAERLGGALRIESAPGSGTILSM"
BASE COUNT          784 a         1500 c         1570 g          833 t
ORIGIN      
        1 cttccagatc aacggccact ggctcgaagt tttcggccag tgctccgtct gccggatgta
       61 acggaatttc ttcccgtccc aatcggcgat caaccggtca acaggtacac gaataccctg
      121 tcgtcccgag ctgcagcgaa ggacctctcc gtgtcagccc tgtatggcgt catagcgaag
      181 aggtccttcg ctgcggctca ggacgacagg gacaagtggt gagcgtccac ttgagatcgc
      241 aatcacggca atcgttcggc gatcagttcg gcgaggtgga ggacgcgcgt cgaggagttg
      301 cgggcgatca ggccgccgcg cagttgcatg atgcagccgg ggttgtcgga gatgacgaca
      361 tcggcggccg tgtcttcggc gttgaccagc ttcttgccga ggatggctgt cgagacctgc
      421 gggtagtcga ccgagaatga gccgccgaag ccacagcaga cgcgcgagtc ctgcatctcg
      481 cgcaattcca ggccgaggac accggccacg atcctccgtg cgccggcacc cagcccaagc
      541 gcgttggccg actgacaggc gtcgtggatc gtgacagtcg gtcccggatt gccgctcgcg
      601 aagtcggctg ggtcgagttg ggcgacctcg tcgacgaatg tcgtgaagtc gatcagacgc
      661 ttgccctgga tcgccgcccg ttcgcgccac tcggagtcgt cgcggaagat gtgctggtaa
      721 tcctggacga tggcggccag acagctggtg ctgttggtta gcacccagtc ggcctcgacg
      781 gtttccagca tccggatcgt ctgctcggcc atgacgcgtc cgtgcgatcg atcgccggag
      841 ttgaacgcca ccagcccgca gcagtgctgg tcggtcggga actgaacgtc gcagccgcag
      901 gcccgcagga ctgtgatgac ggcctcaccc atctctggca gcagccggtc ggtcaggcag
      961 cctgggaaga acgcaacctg cattcccctg gccttgctgc tcggcaggat cgtcgtctcg
     1021 ctcgtcaacg cgccgttgcg gcgaacgcga tcgcgcagcg gatcggtcgc gggtgccgtc
     1081 agtgccctgc cgtcggtcag cgagcccatg ccggggatgg tacggatgac tcgctcgccg
     1141 tctgccacga acgaggacgc gcgggcggcg atgccagccg cgaagccacc gacgcccggc
     1201 ttactccact ggctcaggcc gaccgacttg ctgatcggca gcccgaactt ctcaaccacc
     1261 cgcgaacgaa catcgaggat catgcgggga atcgggatct cggccgggca gactgtctcg
     1321 caggcgttgc aggagacgca caggctctgc gggccggcgg cagccgcgag gccgtggtgg
     1381 gcggcggtca tgaccagccc gatcggaccg gtgtagatgt ggccgaactg atggccgccg
     1441 acgatctggt acggcgggca gacgttcgag caggctccac agcggatgca gtgcaacatc
     1501 tcatgcaggt caggatcctc gcgtgttgcc cagcggccat tgtcgagcag gacgatatgc
     1561 agttccttcg gaccgtggac accgaccgtc agcgtcagct cgatgtcggc gctgcggctg
     1621 gggccggtca cgaatgagac gtagctggtg atcttctggc cggtgcccga gcgcggcagg
     1681 acctggagaa tcgcggtcgc gtcctccagc gacggaacga tcttctcgat ccccagcagc
     1741 gcgatgtgga ccggcggcag cgtcgtcacg agccggccgt tgccttcgtt cgagacgatg
     1801 acgagcgtgc cgctgtcggc aatggcgacg tttgcgccgg agatgccgat gtcggcgtcg
     1861 atgaacgcct gacgtagttt ttcgcgtgcg accttgacca gctcgaccgg atcggccggc
     1921 aactcgacac cgacttcttt cgagaacagc gcggcaacct gctcgcgggt cttgtggatg
     1981 gctggtgcaa tcaggtgcga tggatgctcg tgggccagct gcatgatcca ctcgccgagg
     2041 tcagtctcga cgacctgcag gccggccgct tcgagggccg ggttgagctg gatctcctca
     2101 ctgaccatcg acttgctctt gacgaccagt ttcgcggccc gctgggtcgc gatctcggtg
     2161 atgatccgct gggcgtcctc cggcgttgat gcgatgtgga cgacagcgcc aacggcttcc
     2221 gcctccttgg tgaagcgctc gaccagttcc ggcaggtgct cgattgcgcc ggccttgaga
     2281 tcacggacgc ggcggcggcg ggcggcgaaa tcctggtcgg cgaacgcgtt gatacggcgg
     2341 cggccaaact ccggcagggc gcggcccaac gccgtccgta gcgattcgtc agcgacggcc
     2401 tgcttgacgc gccgcttgaa ggcacgcttg tccggtttct cgatagttcc aaccatctgt
     2461 cgttccgccc tcccgcaaat cggtccgcgt gacgagtata gccgtagata cctgtgttgc
     2521 ctgtcccgac ggacaggatg tagcattcgc gtgacccgaa gcggttgcag tcgcggcggt
     2581 gatctcctcg ccggggtggt gcggccatct gaggcgcgac ggagaccagg cgatggtgcg
     2641 tgacaccgga agcagagcaa gcgtggagtc cgattcccac ccgattcagc aacatcgcga
     2701 tgccttcgcg gcaacctggc gggatctcgt gcgcgagatc agcccgacct atcgggagat
     2761 ggggcaggca cggctgcgcg accgcatcca ggaggggatc gacgcgctgg tcgcacctgg
     2821 ggatgtcgag aacgaggcca gccgccactt tctcaacaat ctcgaatcga tcatcgatca
     2881 ggaacggctc tcggtcgaag aggtcatctc cgggatgctg gccagttttg actgcatgga
     2941 cctggtcatt cccgccgagc agcgctattc gccgccgatg attcactccg agcgggtgct
     3001 gcgatcgatg atcgcccgct tcaccgaatc tgccattggg atcgtcaccc agaagctcga
     3061 agtcgaggct tctgcccagc gcgagagtcg cgcgaggctc ctgtcgctgc aacgcgtcgg
     3121 tgcggccgtc accagctcgc tggagctgga atcgacgctg gagacgatcg tccaggaggc
     3181 ggctgaactg atgaacggcg cgacggcccg cctgcggctg gcggacgaag ttggcgaaca
     3241 cctgcgcctg atcgccagcg ctggagacat cggcgaggat ctgcccggct cggtcgtacc
     3301 ggtcgagacg actctggccg ggctgtgcta tcgcagtggg cggcctgtca tctcgaacga
     3361 cgtggccggc gatccccgct cggactccga actccaacaa ctgacgcgta ctcgctcgtt
     3421 gctgtcggtt ccgctgctgt cgcgcggcac gtcgatcggt gtcctctcga tcacgaacct
     3481 gaccgaccgg ccgttcgtcg atgccgacgc ggagatgctg agcctgtttg cagaccacgc
     3541 cgcagtcgcg atcgagaacg gccgcctctt tcagcaggcg cagagccaga tcaccgagat
     3601 ggagatcctc aatcgcgtct cggccgtcgt ctctgcctcg ctgaaccttt cgcaggtgta
     3661 ccgggcgatc cacgccgaga tcgcccggat catggtcgcc gatgcgttcc tgatcttcct
     3721 gcgcggcccg gagggcggct tcgacctcgt ctacatcgtc gatctcggcc agaccttctc
     3781 gccgcgtcat gacgtgccgc taccgcaggt ctacaagcag tcgatggagc agggcgtccc
     3841 gttcatcgtc gaggcgtcgc gcgaccagtt cctcggcggc tgggagcgct atggcgacat
     3901 gggccgccgt gtccagtcgc tgatggtcgc gccgttgatg cgcggtaccg aagcgatcgg
     3961 cgtcgtgacg acccagtcgt acgcgccgag ctcctatcgc cagcgcgacg ccgacctcct
     4021 ggcgacggtt gccaacgtcg cggcagtggc gatcgagaac gccaagctct acgagcaggc
     4081 gcacgggctg gccgtcgccg acgagcgcaa ccggctggcc cgtgagatcc acgacacgat
     4141 cgctcagggg ctggtcggga tcatcctcca gctcgaagcg ctggccggga tgatcggtga
     4201 cgagccgcgc atgaagcgcc gggtggaccg ggcgatcgac ctggcacgcg tcaacctcga
     4261 cgaggcgcgc cgctcggttc gggatctgcg ggcggccccg ttggagcaca tgtcgctggc
     4321 cgaggcggta cgccagctgg cggcggagca cgaggaagag tgtgacagcg agatcgtcgt
     4381 caatgtcccc gatgcgatgc ccttgctcga caagcagatc gaaacggcgc tgttccgctt
     4441 cgtccaggaa tcgttgacga actgtcgcaa gcacgcgctg ggcgtcccgg tctgggtcga
     4501 tgtcgagatc gccgaatgtg tcacgatctc agttcgtgac tccggccccg gcttcgatgt
     4561 cgaggcctgg cgtagcaaag cgccgctgca tcacttcggc ctgcacggca tgcgcgaacg
     4621 tgccgaacgt ctcggcggcg cgctccggat cgagagcgca cccggctcag gcaccattct
     4681 gagcatg
//
LOCUS       DASUGJ010000154         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1409780, whole genome shotgun sequence.
ACCESSION   DASUGJ010000154 DASUGJ010000000
VERSION     DASUGJ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409780"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..979)
                     /locus_tag="VFV93_03340"
     CDS             complement(296..979)
                     /locus_tag="VFV93_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX5164407.1"
                     /translation="MQTEVLYAPAYALARLSLAPREAATVEAGAMVSMSSDMEIKTSS
                     QGGFLKALKRSTLGGESFFVNTFTASDRGGELTVAPSLPGDMLVLELQNDEYKVQAGG
                     FIASGQGVNVDTKWGGSRSFFGGDGSFFMLSCSGSGPLILASYGALHKMTLGPGQSYT
                     VDTGHVVAFTHDIGFNTRRVGNWKSTILGGEGLVVDLTGPGTLYLQTRSQPAFIGWLT
                     THLPSSQAG"
     gene            1221..1547
                     /locus_tag="VFV93_03345"
     CDS             1221..1547
                     /locus_tag="VFV93_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164408.1"
                     /translation="MNATAMVELSDGSVAIVDSEDLALVSGFDWSLPSSTSSRYPVGY
                     QGRTGGPSAAVYLHRLIAHAGPEDIVYHRNHDTLDNRRENLVVMARMIAPGYLPLDST
                     DSLMAD"
     gene            complement(1563..1973)
                     /locus_tag="VFV93_03350"
     CDS             complement(1563..1973)
                     /locus_tag="VFV93_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5164409.1"
                     /translation="MGDVMLRVLIVEDEKPIRELLHDFLSDEGYETLLAENGQRGVEI
                     ARAEQPDLVLMDLMLPLLDGFEAMRALKDCAGTRDIPVVVMSANSALLQHIGSQSLAE
                     ETIRKPFDLDEVLNVIQLRIGVGPSGEIASVVRR"
BASE COUNT          417 a          721 c          678 g          414 t
ORIGIN      
        1 cgatctccgc gaactcacgc tcgacgtaat ggacgacatc accgacgcgg ctgccggccg
       61 gaaactcgac ctcccgctcc gactcgccga tccgctcacg catgatggcg aaaaagcgca
      121 acgtaatgcg cacctgttcg accaatggaa tgtctcccgc tctgcttgat caccgggcgg
      181 ctccagccag aatcgtatcg cagtgggcgg gattcgggat tcgggacacg ggagcatcga
      241 aggcatcctc cccgctcaac aaccccgaat cccgtgtccc gattcccgtc cgccgctacc
      301 ccgcctggct ggacggcagg tgggtcgtca gccagccgat gaaggcgggt tggctgcggg
      361 tctggagata gagcgtgccg gggccggtca ggtcaacgac caggccctcg ccgccgagga
      421 tcgttgattt ccagttgccg acacgacggg tgttgaaacc gatgtcgtgg gtgaaggcga
      481 cgacgtgacc ggtgtcgacg gtgtagcttt ggcccggccc gagcgtcatc ttatgaagcg
      541 caccgtaact ggcgaggatc agcggaccgg acccgctgca cgagagcatg aagaatgagc
      601 cgtcgccgcc gaagaacgag cgcgagccgc cccatttggt atcgacgttg accccctggc
      661 cgctggcgat gaagccgccg gcctggacct tgtactcgtc gttctggagc tcaaggacca
      721 gcatgtcgcc gggcagcgag ggcgcgaccg tcagttcgcc gcctcggtcg gaggcggtga
      781 aggtgttgac gaagaacgac tccccgccga gtgtgctgcg cttgagtgcc ttcaggaagc
      841 cgccctgcga ggatgtcttg atctccatgt cactgctcat gctcaccatc gctccggcct
      901 cgaccgtggc cgcctcgcgg ggcgccagcg acaaccgggc cagcgcgtag gccggtgcgt
      961 aaagaacttc cgtctgcatc gtgctgtcct ctcgtcctct cgatatctgc agctgccaga
     1021 tgacaacgaa tcgctatcca gaccgctccc agtgagccgc cggcgatgcg tggaacgcgc
     1081 gttgcgtaat ggccttcagg atacatcgaa acggccggca gcggtgctgt cggcacgaat
     1141 cttgcaggga tcgatgtctc tggatcaggt ggagtcgaat gtatcagggc cgctctcatc
     1201 cgggattgaa tggtgcgcca atgaatgcca cggcaatggt cgaactctcc gacggctcgg
     1261 tcgccatcgt cgacagcgag gatcttgcgc tggtctccgg ttttgactgg tcgttgccgt
     1321 caagcacctc ctcgcgctat cccgtcggct atcaggggcg cacgggcggg ccgtctgcgg
     1381 cggtctacct gcatcgtctg atcgcgcacg ccggtcccga agatatcgtc taccaccgca
     1441 accacgacac actcgacaac cggcgcgaga atctggtcgt catggcgcgg atgatcgccc
     1501 ccggctatct gccgctcgat tcgaccgata gtctgatggc ggactgaagc gccggctggc
     1561 ggttatcgcc gaacgacact ggcaatctca ccggacggac ccacgccaat ccgcaactgg
     1621 atgacgttca gcacctcatc gaggtcaaat ggtttgcgga tcgtttcctc tgccagcgac
     1681 tgacttccaa tatgttgcaa cagggccgag ttcgccgaca tgacgacgac cgggatgtcg
     1741 cgcgtcccgg cacaatcctt caacgcgcgc atcgcctcga agccatccag caacggcagc
     1801 atcagatcca tcaggacgag gtccggctgc tcggcgcggg cgatctcaac gccgcgctgg
     1861 ccattctccg ccagcagcgt ctcgtatccc tcgtccgaca gaaagtcatg gagcaactcg
     1921 cggatcggct tctcgtcctc cacgatcagc acccgcagca tgacatctcc caccgctcgc
     1981 ccctcacccg ctcagggtgt acgtacagcg tatcctaagc gaccggtgcc cggatgtcaa
     2041 acgaggtgat aggtagtagg tgataggtgt taggggttgg tcggtacaga cagagacgac
     2101 acgttcctct tgtaccaaac aactcctgat tcggtttctg gtagatcgcg acaccgttct
     2161 gagcggaccg atccggtagg ggcgaggcac tgactcgccc tgtttcccac cgacgccgct
     2221 tgggcggaat
//
LOCUS       DASUGJ010000155         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_20645, whole genome shotgun sequence.
ACCESSION   DASUGJ010000155 DASUGJ010000000
VERSION     DASUGJ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20645"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            508..1110
                     /locus_tag="VFV93_03355"
     CDS             508..1110
                     /locus_tag="VFV93_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5164410.1"
                     /translation="MPVDDERAAIRRLQQGDIRGLESLMLAWQVPAARVAYLITHDRD
                     LAQDIVQSAFLRVYDRIGQFDASRPFGPWFLRIVANDAARLARGQRRIVRFIDRDETS
                     SDLDPVDADPDPETMLLAAETREEVWRALDRLSPEQRGAVVMHYYLDLPDATTADRLG
                     VPAGTVRSRLSIARTRLRSLLSTPESAADSETSAREAGRR"
     gene            1107..2411
                     /locus_tag="VFV93_03360"
     CDS             1107..2411
                     /locus_tag="VFV93_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164411.1"
                     /translation="MSDQQVRDRVRRTLDAAARDSIPDSLDLRLDVLRILAQRHGASP
                     DRLVHPNGHGQVETDDNDETLNHLRAWIGSRHPHQPRIESWPALRRQPWRREVLKLAA
                     AALIFALVGALLVVTMRGTGEQSGTPRPTPATAPLPVQSVSETIPVPGRPYGLTVAAT
                     GVWVPDTNAGVIRRIDPATDDIDMQIDLGATGWQIPAVCGCSSMFAVSTPDGIWETRS
                     SGTSESPVFEIVRLDPATGEIDLTIPLDVLPDKLAVAAGSVWVTSAFHDVVVRIDSRS
                     GARLATIPLDGPLGMIAATESAVWITSLPNDTVTRIDPTTNTIAATISVASPVAVAVV
                     GDAVWVASLDDGALFKIDPATNQVVATVSLIGPDWLTAAGGSLWVHSATSGEVLRIDP
                     ASGDIRARYATGTGVATMAAGEDVLWIALANMQQVLRIDLER"
     gene            2459..>3387
                     /locus_tag="VFV93_03365"
     CDS             2459..>3387
                     /locus_tag="VFV93_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164412.1"
                     /translation="MTRIRTLFIGAALMLTPVLAACGGSSDDPDPTPTATQPPAATAT
                     IFAVTTTATPQATATEATTANPISTGTDALDRIGAVIDTGTTPWSPAAGFGSIWLSIA
                     VDGVSHVARFDPATGEVIATISLDALPAAPGPHSPRADVVIGAEGVWASGWRGTDATS
                     ADGVLVLIDPETNAIARTLELPIAPRSLAVDDAHGTIWASSNVENRVVCMDTESGEVL
                     AKIDVAVAERIELVPDALWVSADDNYGTLTRIDLATYAVVDRVLNGPVYMPGLTYGAG
                     SLWISAVSSDVVGEVQRLDPVSGTVIARIPMSGP"
BASE COUNT          603 a         1134 c         1101 g          549 t
ORIGIN      
        1 ccagccgcgt cggatcggtc agccagttgg tcagcacgat ccgcgtcgcc gggcccggcc
       61 ccaacaacga cctcaccctc caccaggtca tgcggatcac cgtcgacgcc aacgggaatg
      121 ccaccgtctc acccgaccac gtgaccgctc gctgctcctc gatccagccc gtctgagcat
      181 cccgcgccgg tgctcatcgt ggacggtaat gccgccagtc gggcagcgaa ctcactaccg
      241 cagcacgaca cgacctggat cgacgtttcc ggccaggtcg cgtcgtgctg cggtagaagg
      301 agtatcttcg cggatttgct cgactgtggc gcatccacga ccaacgcgtc gctgctgcgt
      361 tgtgcgcctg cctgcttccc aaccgcagag tctcccgcac agcacacaac acgcggagta
      421 ggattcgtcc ctctcagatt ccgatgaaca ttccaggcag gtcgcggata gtacctgtag
      481 acgcagcagc gacatggaat gcgcaggatg ccggtggacg acgagcgcgc cgccatcagg
      541 aggctccagc agggggacat ccgggggctt gagtccctga tgctcgcctg gcaggtgccg
      601 gccgcgcggg tggcctacct catcacccac gaccgggacc tcgcgcagga catcgtccag
      661 tcggcgttcc tccgcgtcta cgatcggatc gggcagttcg acgcgagccg cccgttcggc
      721 ccctggttcc tgcggatcgt cgccaacgac gcggcccggc tggccagggg gcaacgtcgg
      781 atcgtccgct ttatcgatcg cgacgagacg agcagcgacc tagaccccgt cgatgcggat
      841 ccggacccgg agacgatgct gctggctgcc gagacgcgcg aggaagtctg gcgggcactc
      901 gaccgtctct cgccggagca gcgtggggcg gtcgtcatgc actactacct cgacctgccg
      961 gacgccacga cggctgaccg gctcggcgtc ccggccggga cggttcgctc gcgcctctcg
     1021 atcgcacgga cccggttgcg ctcgctgttg agcacaccgg agtccgccgc tgactcggag
     1081 acatctgcta gagaggctgg acggcgatga gcgaccagca ggtgcgcgac cgcgtccggc
     1141 gcacgctcga cgccgccgcg cgagatagca ttcccgacag cctcgacctg cgactggacg
     1201 tgctgcgcat cctcgcccag cgacacggag ccagcccgga tcggctcgtc catcccaacg
     1261 gccacggcca ggtcgaaaca gacgataacg atgagacgct gaatcacctt cgagcctgga
     1321 tcggcagccg ccacccacac caaccgcgaa tcgaaagctg gccggcactt cgccggcagc
     1381 cgtggcgacg agaggtgttg aagttggctg ccgccgcgct gatcttcgcg ctggttggcg
     1441 cgttgctcgt cgtcaccatg cgcggcactg gcgaacaatc cggaactccg cgaccgacac
     1501 cggcgacggc gccgctgccg gttcagtcgg tctccgagac gatccctgtt cccggacgac
     1561 catatgggct gacggtggcg gcgaccggtg tctgggtgcc ggacaccaac gccggcgtca
     1621 tccgccggat cgatccagcg accgacgata tcgacatgca gattgacctc ggcgcgaccg
     1681 gctggcagat cccggccgtc tgcggctgct cgtcgatgtt cgcggtctcg acaccagacg
     1741 gaatctggga gacacgctca tctggaacga gcgaatcgcc agtcttcgag atcgtccggc
     1801 tcgacccggc gaccggcgag atcgatctga cgatcccgtt ggatgtgttg ccagacaagc
     1861 tggctgtcgc cgccggaagc gtctgggtaa cgtcggcgtt ccacgacgtg gttgtgcgga
     1921 tcgactctcg gagcggggca cggctcgcga cgatccccct cgacgggccg cttgggatga
     1981 tcgcggcaac cgagtcggct gtctggatca cgagcctccc gaacgacacg gtgacgagga
     2041 tcgatccaac gacgaacacg atcgccgcga ccatctcagt cgcctctccg gtcgcggtcg
     2101 cggtcgttgg cgatgccgtc tgggttgcga gtctggacga cggcgcattg ttcaagatcg
     2161 acccagcaac caatcaggtg gtggcgacgg tcagcctcat cggaccggac tggctgacag
     2221 ccgcaggcgg gtctctttgg gtccacagcg cgacaagcgg tgaggtgcta cggatcgacc
     2281 cagccagcgg cgatatacgc gcccgctacg caaccgggac tggggtggcg acgatggcag
     2341 ccggcgaaga cgtgctgtgg atcgcgctgg caaacatgca acaggtgcta cggatcgatc
     2401 tcgagcgata gcgatcgact gtcccgcaac ggggatcacg aacgagagag gtggacagat
     2461 gacacgcatt cgcaccctct tcatcggagc ggcactgatg ctgacaccgg tactggccgc
     2521 ctgtggcgga tcgagcgacg atcccgatcc gacaccgacc gcaacacagc cacccgccgc
     2581 aacagcgacg attttcgccg tcacaaccac ggcaacgccc caggcgacgg cgactgaggc
     2641 gacgacagcg aaccctatct cgacaggaac tgatgctctc gatcgcatcg gagccgtcat
     2701 cgacaccgga accacgccct ggagcccggc cgccggattc ggctcaatct ggctctcgat
     2761 tgcggttgat ggcgtgagtc acgtggcacg gttcgacccc gctaccggtg aggtcatcgc
     2821 aacgatcagc ctcgacgcac tccccgccgc tccgggcccc cactcgccga gggctgacgt
     2881 cgtgatcggc gcggaggggg tctgggccag cggctggcga ggtaccgacg cgacctcggc
     2941 tgatggagtc ctcgtcctga ttgatcccga aacgaacgca atcgcgcgga cgctcgaatt
     3001 gccgatcgca ccacgctcgt tggcggtcga tgatgcgcac ggcacgatct gggcatcctc
     3061 gaacgtcgag aaccgagttg tctgcatgga cacggagagt ggcgaggtgc tggcgaagat
     3121 cgatgttgcc gtcgcggagc ggatcgagct ggttccggac gcgctctggg tctccgccga
     3181 cgacaactac ggcactttga cgcggatcga cctggcgacc tatgcagtgg tggatcgagt
     3241 gctgaacgga cctgtctaca tgccaggact gacctatggt gcgggatcgc tctggatctc
     3301 ggcggtgagt tccgacgtgg ttggtgaggt tcagcggctc gatccggtca gcggcacggt
     3361 tatcgctcgc atcccgatga gcgggcc
//
LOCUS       DASUGJ010000156         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1966601, whole genome shotgun sequence.
ACCESSION   DASUGJ010000156 DASUGJ010000000
VERSION     DASUGJ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966601"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..>3136)
                     /locus_tag="VFV93_03370"
     CDS             complement(6..>3136)
                     /locus_tag="VFV93_03370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5164413.1"
                     /translation="LLDGLKGTILELSAINELSLLGLAALILVALAGAVVIHRGATEY
                     RPGKPAPAAVQFTGQFVSVRSIGFLAGSLLLVLGAFAYQQYLWHVELPIPDDAVGIAL
                     TREAAAASISDELREALFVQGDSDQIVIRELPVKFDAGDVEKARAMGKRIGARAVVIY
                     RSAGDGDQQQYSAYVVFTDPAVGVTVGTAPVSTAESDAVVQQGGIIIQQSVPVPVLRT
                     ASLAQLVDATAGIIAHDEGSYGAAIQLLEDARAADNEGRDVGLIDYYLGSAYWSTSQL
                     EPAAEAYQRAIDGYEKRRAAGRISAQDALILAQAYLDLGRVRLNEDDDGWADAAQAIF
                     ESALDLRDDLLARRSQLERPSDVHVTYAQIYAMLADLYRLRGATDEQQHWETRAREEA
                     AAIGADGGDDSGIAVQEAAARGLAGDCFGALAAVDRALDADPDNLDALVLAATIQMID
                     GRPDLAEAAIERAVTVRPDSVTAHDLAAMSMLMRGIGNRTYVEPSYISDAEDRFRDLL
                     AVDPSNVNAHRWIAELVKMRSDGQLLDSTAVSSGDEINSTKSSALWQADPEHYDAAVD
                     LLGQAIEEYRIIAYELNPDDLGARMVLAKLYVERMNLLYYHLPVLQTAGDTAGIERDS
                     ELIGEDMTRVREATDGLRSGEVPGSRLEVLQAWSSFIRAMEYETTRILFYETDTSDAD
                     TTDDERAQATLDEWTAAIDAGLAVVKAEPLNGPGEKDAASYIYLKKALIHLFNSETDA
                     AQQPQAMFVQLQTEVADYYRERSGHNQTLCADMREVERGDTLTASGSYSQATDAYRQA
                     LSLNADNALALDGLAYALFHNGDVTGAIDAAHQATDIYPDNPRPWARLGLYALVNDDT
                     ATRDAAYGRFLSLTAQRPLQERLALAKEAVTDLQDLVRDEPQRGAAALELLPQLRGFA
                     DGISDAGDAYQYPQLYSELGKFALLAGDTNAAQTLLHRGIELDAHQPIAKVRLAMAVL
                     IDGDRATDEVQAVVDELNDPLWAETEGVEAFGRDDLTELAQAEIGEFVAVRPDDGNMF
                     DSLVQQLNRSVE"
BASE COUNT          481 a         1071 c          995 g          589 t
ORIGIN      
        1 agagcttatt caaccgagcg attcaactgc tgaacgaggg agtcgaacat gttgccgtca
       61 tctggacgca ccgccacgaa ttcgccgatc tccgcctgag ccagttccgt cagatcatcc
      121 cgtccgaacg cctcgacgcc ctccgtttcc gcccaaagcg gatcattcag ttcgtcgacc
      181 accgcctgaa cttcgtctgt cgctctatcg ccatcgatca agaccgccat cgcgagccgg
      241 actttcgcga ttggctgatg ggcgtccagt tcgatcccac ggtgtaggag tgtctgtgca
      301 gcatttgtgt caccggccag cagcgcgaat ttgcccagtt cgctatacag ctgcggatac
      361 tggtacgcgt cgccggcgtc cgagattccg tcggcaaatc cccgcagttg cggcaatagt
      421 tccagcgcgg ctgcgcctcg ttgcggctca tcccgtacca gatcctgtag atcggtgacc
      481 gcctccttcg ccagtgccag ccgctcctgc agcggacgtt gggcggtcaa cgagagaaat
      541 cgcccatacg ccgcgtcgcg ggtggccgtg tcgtcgttca ccagcgcgta tagcccgagt
      601 cgcgcccatg gacggggatt gtccggatag atgtcggtcg cctgatgggc ggcgtcgatc
      661 gcgccggtga cgtcgccatt gtggaagaga gcgtacgcga ggccgtccag cgccagcgcg
      721 ttgtcggcat tcaggcttaa cgcctgccgg taggcgtcgg ttgcctggct gtagctgccg
      781 gatgctgtca gcgtatcgcc gcgctcgacc tcgcgcatgt cggcacagag ggtctggttg
      841 tggcctgagc gctcgcggta gtagtcggcc acctctgtct ggagctggac gaacatcgcc
      901 tgcggctgct gggccgcatc ggtctcgctg ttgaagaggt ggatcaacgc cttctttaga
      961 taaatgtagg aggccgcgtc cttctcgccc gggccattca gtggctcggc tttgacaacc
     1021 gccagtccgg cgtcaatggc ggctgtccac tcatccagcg ttgcctgggc ccgttcgtcg
     1081 tcggtcgtgt cggcgtcgct ggtgtcggtc tcgtagaaga ggatgcgcgt cgtctcgtac
     1141 tccattgctc ggatgaagct actccacgcc tgaaggacct ccagccggct ccccggtacc
     1201 tcgcccgacc gcagcccgtc agtcgcctcg cggacccgcg tcatgtcctc gccaatcagc
     1261 tccgagtccc gctcgattcc ggcggtgtcg cccgctgtct ggaggaccgg caggtggtaa
     1321 tagagcaaat tcatccgctc gacgtagagc tttgccagca ccatccgcgc gccgaggtca
     1381 tcaggattca gttcgtaggc gatgatgcgg tactcctcga tcgcctgccc gagcaggtcg
     1441 acggcggcgt cgtaatgttc cgggtccgct tgccagagtg cgctgctctt ggtcgagttg
     1501 atctcatcgc cgctgctgac ggccgtactg tccagtagct ggccgtcgct gcgcatcttc
     1561 accagctctg ctatccagcg gtgggcgttg acgttggagg gatcgactgc cagcaggtcg
     1621 cgaaagcgat cctcggcgtc actgatgtag cttggctcga cgtacgtccg gttgccgatc
     1681 ccgcgcatca gcatcgacat cgcggccaga tcgtgggccg tcacggagtc gggccggacg
     1741 gtgaccgccc gttcgatcgc cgcttcagcc agatcaggcc gcccgtcgat catctggatc
     1801 gtcgccgcca ggaccagtgc gtcgaggttg tccgggtcgg catccagcgc ccggtcgacg
     1861 gccgccagcg ccccgaaaca gtcgccagcc agcccgcgtg ccgccgcctc ctgaacggcg
     1921 atgccgctgt cgtcgccacc atcggctccg atcgctgctg cttcctcacg tgcccgcgtt
     1981 tcccagtgct gctgctcgtc ggtcgcgccg cgcagccggt agaggtcggc cagcatcgca
     2041 tagatctgcg cgtaggtgac gtggacatcg ctgggccgtt ccagttgcga gcggcgagcg
     2101 agcagatcgt cgcgcaggtc cagcgccgac tcgaagatcg cctgggccgc gtctgcccag
     2161 ccgtcgtcgt cctcgttcag ccggacacgg cccaggtcga gatacgcctg cgccaggatc
     2221 agcgcatctt gggcggaaat cctgcccgcg gcccgccgtt tctcgtagcc gtcgattgcc
     2281 cgctggtacg cctcggccgc cggctcaagc tgcgatgtcg accagtaggc gctgccgagg
     2341 tagtagtcga tcagcccgac atcgcgccct tcgttgtcgg ccgcccgggc gtcctccagc
     2401 aactgaatcg ctgcgccata gctcccctcg tcgtgggcga tgatcccggc cgtggcgtcg
     2461 accagttgcg ccagcgaggc ggttcgcagc accggcaccg gcacgctctg ctggatgatg
     2521 attccgccct gctggacgac cgcgtcgctc tcggcggtac tgaccggagc ggttccgacc
     2581 gtcacgccga cggccggatc ggtgaagacg acgtaggcgg aatactgctg ctggtcgcca
     2641 tcccccgcgc tgcggtagat gacgaccgcc cgcgcgccga tccgcttgcc catcgcgcgg
     2701 gccttctcga cgtcgccggc gtcgaacttg accggtagct cgcggatgac gatctgatcg
     2761 ctgtcgccct ggacgaagag cgcctcgcgc agctcgtcgg agattgacgc ggccgccgcc
     2821 tcgcgcgtca gcgcgatccc gacggcgtcg tccgggatcg gcagctcgac atgccagaga
     2881 tactgctggt aggcgaacgc gcccagcacg agcagcagcg aacctgccag aaagccgatc
     2941 gagcgcacgc tgacgaactg gcccgtgaac tgcactgcgg ccggcgcagg cttgccgggc
     3001 cgatactcgg tcgcgccgcg atggatgaca accgccccgg ccagcgcgac caggatcagc
     3061 gccgccagcc ccagcagcga gagttcgttg atggccgaca gctcgagaat cgtccccttc
     3121 agaccgtcga gcaact
//
LOCUS       DASUGJ010000157         4790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1827399, whole genome shotgun sequence.
ACCESSION   DASUGJ010000157 DASUGJ010000000
VERSION     DASUGJ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827399"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1893)
                     /locus_tag="VFV93_03375"
     CDS             complement(154..1893)
                     /locus_tag="VFV93_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX5164414.1"
                     /translation="MDRREISRRKVLQGGAALAGLAVLHSQLPALASQRRAGLDSIPW
                     PAQVTQDFPTRPGEEILPWLDQPAENPVPDILGTPILWENVDSWITPNPEFFTVKHYT
                     QPEIDPATWQLTISGLVGNPLTLSLADLMARESREVTFTLECSGNHGLPFLTGAIGNA
                     VWTGTPLAPLLQEAGILAEGSEVVFFGTDSGPETVNGLELTEQFARGMSLAEAMDPRN
                     ILCWGMNGEPLPAGHGAPVRLIAPGWYGIANVKWLQRIEILDRPWEGRFMAREYVTIR
                     EEERNGNRLARYTLVGHARLKSAPARVTRLDGQYRIVGAAWGAPIAEVEVQIDGGPWQ
                     AATIEGQDDSHNTWEFWTLDWDQPTPGEHTITTRAIDTDGNIQPAMDDPIIANKLTFW
                     ESNGHVTRRVNTGAQTFPETGHTLSGEFLTFWQQHGGLATFGYPITEAFEEDGRTVQY
                     FERQRFELHPENAAPYNVLLGLLGAEALDGAPHPPVQPGDGSSCDFHAATGHNACGRF
                     RDVWQQGGLMIYGYPITEEFEQDGLTVQYYERARFEHHPGNQAPYDVLLGLLGYEALE
                     SRYGGNLPPGAVE"
     gene            complement(2083..2415)
                     /locus_tag="VFV93_03380"
     CDS             complement(2083..2415)
                     /locus_tag="VFV93_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5164415.1"
                     /translation="MQAIMIDRSEDSRQLALLHGAECQLIDAIATARETIARCEQALA
                     SLSEARSQIAAERLSPPSPAARFTSLADTLFLSPRTIERHIANIYLKIDAHSKAEATA
                     YARRNGIV"
     gene            complement(2664..>4790)
                     /locus_tag="VFV93_03385"
     CDS             complement(2664..>4790)
                     /locus_tag="VFV93_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5164416.1"
                     /translation="ADLGRTQIPALLQQVIEARLARLDETTQRLLEVAAVIGQDVPLQ
                     LWSVVAEADDASLAQAIEDAVAAGLLTVSDGGTILRFTHALLRETIYERQMLLRRRPW
                     HRRAAEALIAAPHPDPDAIAYHLEQVNDPRLVDWLIRSGERAQQRFAWPAAARQFDRA
                     QAMLASDPDRDAERAWLLFRIAVLVRNTDQSGSLTYLDEAEQIATALGDPVLTHLSRA
                     HRGLVLCYGRDVRQGLIDLEAGTRALERLDEEDVHRLRSVTADWEAILGVQGRATLAQ
                     WLANVGRFQQARELLLSWSDSERDAAPDAHRAAGIIASSLGEPELARRWFRRSRAVYE
                     TRNDRFQAGDDAFHELLDFHIPYAADRAGERRALVRTLLDLWTDPTDPDQSTWATQAI
                     EAIECVISGQWSQADEWYDQHRTDWRFPLSPFGFWWLLLARHRGEAEVAWAAIRAALP
                     DGPATEPGGQGYAVLAGLQLMAAELALDASDLPRSRAWLEAHDRLLEWSIAVRGRAEN
                     HLAWARYHQRAGDGIAARDRAERALAQATDPHQPLALIAAQRFFGQLAIADQRYDLAE
                     QRLTASLTLAAACAAPFERALTLAVLAELHAGTGDLDAAQRVIEEARSICEPLRAVPT
                     LAHLNALEAQLETQRGATNLPAGLSVREAEVLRLVAQGLSDAEVAEQLFIARRTVNTH
                     LTSIYTKLGVNSRVAATRFAVEHHLT"
BASE COUNT          770 a         1562 c         1596 g          862 t
ORIGIN      
        1 ccttcaacct gctcggcgac tccctccgcg atgtcctcga cccaacgttg cgcgagtgac
       61 cgcgcttctc tcccggtagg agctggtaag tggtctccgt accagcccct accgggagag
      121 aagcgcgtat cctgacaccg actttcgaaa accttactcg acggcgcccg gcggcaggtt
      181 cccgccgtag cgggattcca acgcctcgta gccgagcagg ccgagcagca cgtcgtacgg
      241 cgcctgattg ccggggtggt gctcgaagcg ggcgcgctcg tagtactgga cggtcagccc
      301 gtcctgctcg aactcctcgg tgatcgggta gccgtagatc atcagcccgc cctgctgcca
      361 gacgtcgcgg aagcgcccgc aggcgttgtg gccggtggcg gcgtggaagt cgcagctgct
      421 gccgtccccc ggctggaccg gtgggtgtgg cgcgccgtcc agcgcctcag cgccgagcag
      481 accgagcagg acgttatacg gggcggcgtt ctccggatgc agctcgaagc gctggcgctc
      541 gaagtactgg acggtccggc catcctcctc gaacgcttcg gtgatcggat agccgaaggt
      601 cgccaacccg ccgtgctgct gccagaacgt caggaactcg cccgacagcg tgtgtcccgt
      661 ctcagggaac gtctgtgcgc cggtgttgac ccgccgtgtg acgtggccgt tgctctccca
      721 gaaggtcagc ttgttagcga tgattggatc gtccatagcc ggctggatgt tgccgtcggt
      781 atcgatcgcg cgtgtggtga tggtgtgctc gccgggcgtg ggctggtccc agtccagcgt
      841 ccagaactcc caggtgttgt ggctgtcgtc ctgcccctcg atcgtggctg cctgccaggg
      901 gccgccgtcg atctggacct cgacctccgc gatcggtgcg ccccaggcgg cgccgacgat
      961 gcggtactgc ccgtcgagcc gcgtgacccg ggccggagcc gacttgagcc gggcgtggcc
     1021 gaccagcgtg taacgcgcca gccggttgcc gttgcgctct tcctcgcgga tcgtgacata
     1081 ctcgcgggcc atgaagcggc cctcccaggg ccggtccagg atctcgatcc gctggagcca
     1141 cttgacgttg gcgatgccgt accagcccgg cgcgatcagt cgaacgggtg cgccgtgtcc
     1201 ggccggtagt ggctcgccgt tcatccccca gcagaggata ttgcgtggat ccatcgcctc
     1261 ggccagcgac atgccgcgcg cgaattgctc ggtcagttcg agaccgttga cggtctcggg
     1321 gccgctgtcg gttccgaaga agacgacctc gctgccctcg gcgaggatgc ccgcttcctg
     1381 aaggagcggt gcgagtggtg tgccggtcca gacggcattg ccgatcgcgc cggtcaggaa
     1441 cggcagcccg tggttgccgg aacactccag cgtgaaggtc acctcacggc tctcgcgcgc
     1501 catcaggtcg gccagcgaga gcgtcaacgg attcccgacc aggccgctga tcgtcagttg
     1561 ccaggtcgcc ggatcgatct ccggctgggt atagtgcttg acggtgaaga actccgggtt
     1621 gggtgtgatc caggaatcga cgttctccca gagaatcggc gtcccgagga tatccggcac
     1681 cggattctcg gccggctggt ccagccatgg caggatctct tcgccgggcc gggtcggaaa
     1741 gtcctgcgtg acctgggccg gccaggggat gctatccagg ccggcacggc gctgcgacgc
     1801 cagcgccggc agctgcgagt ggaggacggc cagtccggcc agcgccgcgc cgccctgcaa
     1861 caccttcctg cgcgatatct cgcggcgatc catgacggaa ctcccttcct tgaacgtgtc
     1921 tgcctgaacc ggaggaatcg ccggatgccg gcgacgattg ccgcaaacgc ccttcgattc
     1981 ccccggcatg catcacagta gggcggacca tacccatgtc gcatccggga aggtatgtag
     2041 tttggggtat gtaattcctg agattgggtg ttgcggctgt cgtcagacga tgccattgcg
     2101 ccgcgcgtag gcggtcgcct cggctttgct gtgcgcgtcg atcttgaggt agatgttggc
     2161 gatgtggcgt tcgatcgtgc gtgggctgag gaagagcgtg tcggcgagtg acgtgaagcg
     2221 cgctgccgga gaaggcggcg acagacgttc ggcagcgatc tgcgagcggg cttcggacag
     2281 actcgcgagt gcctgctcgc agcgcgcgat cgtctcccgt gcggtggcga tcgcgtcgat
     2341 gagttgacat tcggcaccgt gcagcaacgc cagctgccgg ctgtcctcac tgcgatcgat
     2401 catgatggcc tgcatcggca acctcctccc ggcgctgccc gctatcaatc ctgggctggt
     2461 tcgacgatgg gccgatcggc gtggctcacc acacacaact gacacgatgg gtgacatgtt
     2521 gcttcaagat gtcatcgatg cggtcgtgac accgtgcgtc gccgatccaa cagggctatt
     2581 ctgggcgcaa tgccacccga ctcgaatcgt gcgaataatg tgtttcgatc tgaggggcgg
     2641 cacgtgatag tacgtagagg tggtcaggtc agatggtgct cgacggcaaa gcgggtcgcc
     2701 gcgacgcgtg agttgacgcc cagcttggtg tagatcgacg tcaggtgggt gttgaccgtc
     2761 cgacgcgcga taaacaactg ttcggcaact tccgcatcag acagaccctg agcgaccagt
     2821 cgcagcacct cggcttcgcg cacgctcaat ccggcgggga ggttggtggc cccgcgttgc
     2881 gtttcaagct gcgcctccag tgcgttgaga tgtgccagcg tgggtaccgc ccggagcggc
     2941 tcgcagatcg agcgtgcctc ctcgatgacc ctttgagccg catcaagatc cccggtgccg
     3001 gcgtggagct ccgcgagcac cgccaacgtc agagcgcgct cgaacggtgc cgcacaagca
     3061 gcggccagcg tcaacgacgc ggtaagccgt tgctcggcca gatcgtagcg ctgatccgcg
     3121 atggcgagct ggccgaagaa acgctgggcg gcgatcagtg ccagtggctg atgcggatcg
     3181 gttgcctggg ccagcgcccg ctctgcccga tcgcgtgccg cgatcccatc gccggctcgc
     3241 tggtgatagc gcgcccaggc gaggtggttc tcagcccggc cacggacggc gatgctccat
     3301 tcgagcaggc ggtcgtgcgc ttcaagccag gcgcgactgc gcgggaggtc ggatgcatcc
     3361 agcgccagtt ccgcagccat gagctggaga ccggcgagca ctgcgtagcc ttgcccgcca
     3421 ggttcggttg ccgggccatc gggcagcgcc gcccgaatcg ctgcccaggc aacttctgcc
     3481 tcacctcgat gacgcgcaag gagcaaccac cagaaaccga acggtgagag cgggaaccgc
     3541 cagtctgttc ggtgctggtc gtaccactcg tccgcctgtg accattggcc gctgatgacg
     3601 cattcgatcg cctcaatcgc ctgcgtcgcc cacgttgact ggtctggatc ggtcgggtcc
     3661 gtccacagat ccagtagcgt tcgcaccagt gcccggcgct ccccggcccg atccgcagca
     3721 tagggaatgt ggaagtcgag cagttcgtgg aaggcatcgt ctcctgcctg gaaacggtcg
     3781 ttgcgggttt cgtagacggc gcgggaccgt cgaaaccagc gacgcgccaa ctctggctct
     3841 cccaggctgg aggcgatgat gccggctgcc cggtgcgcat caggcgctgc gtcgcgctcg
     3901 ctgtcggacc agctcagcag taactccctg gcttgctgga agcgaccaac gttcgccagc
     3961 cactgcgcca gcgtcgcgcg tccctggact cccagaatcg cttcccagtc ggccgtcaca
     4021 ctccgtagcc tgtgaacgtc ctcttcgtca agccgttcga gcgcccgcgt ccctgcttcg
     4081 aggtcgatca gcccctggcg aacgtcccgt ccgtagcaga gaaccagtcc tctgtgcgct
     4141 cgggagagat gcgtgagaac aggatcaccg agcgccgtcg cgatctgttc tgcctcgtcc
     4201 aggtacgtca ggctcccgga ttgatcggta ttgcgaacca ggacagcgat tcgaaacaac
     4261 agccaggcgc gttcagcgtc gcgatccgga tcactggcga gcatcgcctg cgcccgatcg
     4321 aactggcgcg cggctgccgg ccaggcaaag cgttgttgcg cccgctcgcc tgaacggatg
     4381 agccagtcaa ccagacgcgg gtcgttcacc tgctcgaggt gataggcgat ggcatcagga
     4441 tcgggatgtg gcgcggcgat caacgcctcg gctgcacggc ggtgccaggg gcgccggcgc
     4501 agcagcatct ggcgctcgta gatcgtctcg cgaagcagcg cgtgcgtgaa gcgcaggatc
     4561 gtgccgccgt cggacacagt gagcagccca gccgcgacgg cgtcttcaat cgcctgtgcc
     4621 agactcgcgt catctgcctc cgccacaacg ctccagagct ggagcggtac gtcctgtccg
     4681 atcacggccg caacttcgag caggcgctgc gtcgtctcgt ccaagcgcgc cagtcgagcc
     4741 tcgatgacct gttgcagcag cgccggaatc tgcgtccgtc ccagatcggc
//
LOCUS       DASUGJ010000158         7190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1271212, whole genome shotgun sequence.
ACCESSION   DASUGJ010000158 DASUGJ010000000
VERSION     DASUGJ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271212"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VFV93_03390"
     CDS             <1..820
                     /locus_tag="VFV93_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX5164417.1"
                     /translation="GSNFGVVTSFEFELHPLGPEVMLCAVAYHLDDAPMVLPKWRDLQ
                     TTMPEEVTTLAVLWSVPMGFPPELVGTPVVIIAAVYAGPPEEGERVTMPFRQLATPLL
                     DLSAREQWTAIQSGFDPFFPKGSLYYWKSTYVEAFSDELIDALCRLAAERPSPRTTMD
                     IWPQDGAPMRVGATETAFGRRPPFLIAFESSWDDPADNEANIRWAREAYASMSRFQSS
                     GIYLNFPGFGEEKDDLVRRAYGANYDRLVAIKTAYDPGNLFRMNINIAPDGTRG"
     gene            858..1169
                     /locus_tag="VFV93_03395"
     CDS             858..1169
                     /locus_tag="VFV93_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5164418.1"
                     /translation="MYGTIGQARIKDGKLAEVLTLGETWNRERGPKVPGAIAAYWFQA
                     DNDPNLITIVAIFRDRETYHANANDPGQDAWYQQLRPLLATDTTWNDGEVVFAGMFNG
                     I"
     gene            complement(1268..2326)
                     /locus_tag="VFV93_03400"
     CDS             complement(1268..2326)
                     /locus_tag="VFV93_03400"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX5164419.1"
                     /translation="MLIIDGHLDLAMNALLWNRDLKQTVAATRAHEAGMTQKGRAAGT
                     VAFPELRSGNIGVTLCTVIARVDQPGAVRSGYRTHEIAYGHAQGQLAYYLELQRQGVI
                     RMLDNWPALEAHLADWEANPDTTPLGMILTMEGADPIVEPSQVGPWYEQGLRVISLAH
                     YGPSAYAKGTESVGGLTQMGRDLLREMDNLGLTLDITHLSDDSFWEAVELFKGPVIAT
                     HSNCRALVPGDRQLSDDMLRHLIDRDVVIGAALDAWMLEPNWIRGETTPERVTLETVV
                     DHIDHVCQLAGNARHAAIGTDLDGGYGTEQCPNDLDTIADLQKIPDILRSRGYSEPDI
                     ELIMHGNWTNHFKRVWSA"
     gene            2617..4488
                     /locus_tag="VFV93_03405"
     CDS             2617..4488
                     /locus_tag="VFV93_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5164420.1"
                     /translation="MEDNRNVQKLLDDITRHGLSRRAVLKRAVALGLAAPTISMLLAA
                     CGGEDDDDDDGGDSPTATSASGAATTPAEATEAPEETPEAEATEASAETPAAGETPAG
                     ETPAAGETPAAGETPATSAENDIWADGKAGGRLKIASIGEPATLDEHQTTAGITAEIG
                     YCMYETLFAYDANYLPVPMLAESYDVSDDGTVHTFKLRQGVPFHNGEEMTSADVVASV
                     ERWGRISGVGKNVMAATTSIEATDDYTVTWTTTKPYGTILIALSNNTQGCVIYPKSLL
                     DTQSDDPIASADIVGTGPYKLVEHQPDVHILMERFEDYAALEGGALGSYGGHKYAWVD
                     EIEFVPVPDEASRVAGMQAGDYHIGATIGNDHYDVLKGSGDLLVEILPPSQWDVFFLN
                     WAQPMFADENNRKAFQACLDHLPILQNSRGGGDFVALDPSLMQKPSAFYSTAGGELYN
                     IHDVELAKQYLADSGYDGTPVRFMCTQEYAYMYGAAIIAQQQMEEAGFVVDLQVTDWA
                     TVLERRAKKEEWDIFVTGHGFVPDPTQITYIGQMNIYPGWWNDPTALDLAAQLASETE
                     FEKRYEIYEQIQQLAYEQVPAVKTGDSSTIVVWAGSVGGYGAPIQRGVPYWNVWLDE"
     gene            4545..5495
                     /gene="nikB"
                     /locus_tag="VFV93_03410"
     CDS             4545..5495
                     /gene="nikB"
                     /locus_tag="VFV93_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HEX5164421.1"
                     /translation="MLTYVIRRLILLIPVIAIVGTIVFVLIHLTPGDPAAVILGPDAT
                     PEQIESLRDRLGLNEPLYEQYVHWFAGAVRLDFGDSIFLGQPVTQSLLDRAQPTLLLT
                     LYALTIQVLIAIPFGVISAVKHNSLLDRILMVLSISGAAIPSFFLGIMLILFFAVRLQ
                     WLPSGGFTSITEDPVQHFKQMILPAFALGFTSAGLLARLVRSSMLDVLKEDYIRTAQA
                     KGLPQRMMVVRHALRNALIPAITVIGYSLGGLLGGAVVTETVFTIPGMGRLVVQSVAR
                     RDFPVIQGAILIIAAIYVLANLLVDVLYVYIDPRVRYGND"
     gene            5482..6411
                     /locus_tag="VFV93_03415"
     CDS             5482..6411
                     /locus_tag="VFV93_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164422.1"
                     /translation="MATTEHTETAPLANALSTRPVQRGLLRNITPASRFYWVRVLLTN
                     PMAVASGTILLIIILAAIAAPVLTSHDPKFLDPGIRLQGLTGEHWFGTDGSGRDVFAR
                     VLYGARVSLIVGLSVMAMAASIGAVLGLISGYYQRLDTPIMRVMDGLMSFPSILLAIA
                     IMASLGPSTINVIMALTVVYIPGVARLVRGTTLVTKQQAYVESARAVGVRDHVILRRY
                     IFANTLSPLIVQCTFVVAFAIISEASLSFLGAGVPPETPTWGNMLRDGQQFIQIAWWM
                     AVFPGSALFILVLALNLFGDGLRDALDPRMRRR"
     gene            complement(6623..7051)
                     /locus_tag="VFV93_03420"
     CDS             complement(6623..7051)
                     /locus_tag="VFV93_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164423.1"
                     /translation="MIRLTKRRLLMLAACTLLVWVSFGALAAANTVDPSSADDQTLAF
                     EPPEQEGDCGTITGNNVIMGTSGPDILFGGNGRDCVVGLGGIDILHGGNQSDILLGGP
                     GDDILFGDNSADHLDGGPGFDICYGGNSTDTFVNCEIWVQ"
BASE COUNT         1268 a         2360 c         2316 g         1246 t
ORIGIN      
        1 cggcagcaac ttcggggtcg tgacctcgtt cgagttcgag ctgcacccgc tcggccccga
       61 ggtcatgctc tgcgccgtcg cctaccacct cgacgatgcc ccaatggtgc tgccgaagtg
      121 gcgcgatctg caaacgacga tgccggagga agtcacgaca ctggccgtcc tctggagcgt
      181 gccgatgggc ttcccgccgg aactggtcgg aacaccggtc gtcatcatcg cagccgtcta
      241 cgccggtccg cctgaagaag gcgagcgcgt gacgatgccg ttccgtcaac tggcgacgcc
      301 actgctcgac ctgagcgcac gcgagcagtg gacggcgatc cagtcgggct tcgatccgtt
      361 cttcccgaag ggatcgctct actactggaa atccacctac gtcgaggcgt tcagcgacga
      421 gctgatcgac gcgctctgcc gcctggccgc cgagcggccc tcgccacgca cgacgatgga
      481 catctggccg caggacggcg cgccgatgcg cgttggcgca accgagacgg cgtttggtcg
      541 ccggcccccg ttcctgatcg ccttcgagtc gtcatgggac gatccggccg acaacgaggc
      601 caacatccgc tgggcgcgcg aggcgtacgc ctcgatgtcc cgcttccagt cgagcgggat
      661 ctacctcaac ttccccggct tcggcgagga gaaggacgac ctcgtccgcc gcgcctacgg
      721 agccaactac gaccggctgg tcgccatcaa gacggcctac gaccccggca acctgttccg
      781 gatgaacatc aacatcgcgc cggacggaac gcgcggctag cgcacgacca gcgccaattc
      841 gctgaaagga gcgagacatg tacgggacga tcggacaagc gcgcatcaag gacggcaagc
      901 tggcggaggt gctgacgctc ggtgagacgt ggaaccgcga gcgcgggcca aaagtgccgg
      961 gggccatcgc agcctactgg ttccaggcgg acaacgaccc gaacctgatc acgattgtcg
     1021 ccatcttccg cgaccgcgag acctatcacg ccaacgccaa cgaccccggt caggacgcct
     1081 ggtaccagca actccgcccg ctgctggcga ccgacacgac ctggaacgac ggcgaagtcg
     1141 tcttcgcggg gatgtttaac gggatttaga cgtgaggtga cgggattcgg gattcgggat
     1201 tcggggggtc ttgaagacaa tatgtccgtt cgatgacccc ggttcccgaa tcccgaatcc
     1261 cgacacctca cgcactccaa acccgcttga agtgattcgt ccagttgccg tgcatgatga
     1321 gctcgatgtc gggttcgctg tagccacggc tgcggaggat atcggggatc ttctggaggt
     1381 cggcgatcgt gtcgaggtcg ttggggcact gctcggtgcc gtagccgccg tcgaggtcgg
     1441 tgccgatcgc ggcgtggcgg gcgttgccgg ccagctggca gacgtggtcg atgtgatcga
     1501 cgaccgtctc cagcgtgacc cgctctggtg tcgtctcgcc gcgaatccag ttcggctcca
     1561 gcatccaggc atccagggcg gcgccgatca cgacatcgcg gtcaatcagg tggcgcagca
     1621 tgtcatctga cagctggcgg tcgccgggga cgagcgcgcg gcagttggag tgggtcgcga
     1681 tgacggggcc tttgaacagc tcgacggcct cccagaagct gtcgtcggag agatgggtga
     1741 tatcgagcgt cagcccgagg ttgtccatct cgcgcagcag gtcgcggccc atctgggtca
     1801 gcccgccgac gctctcggtg cccttggcgt aggcgctggg gccgtagtgg gccaggctga
     1861 taacccgtag cccctgctcg taccacggtc cgacctgcga cggctcgacg atcggatcgg
     1921 cgccctccat cgtcaggatc atccccagcg gcgtcgtgtc ggggtttgcc tcccagtcgg
     1981 ccaggtgcgc ctccagcgcc ggccagttgt ccagcatccg gatgacaccc tgccgctgga
     2041 gttccaggta gtacgcgagc tgcccctggg cgtggccata ggcgatctcg tgggtgcggt
     2101 agccgctacg gacggcgccc ggctggtcga cgcgggcgat gacggtgcag agcgtgacgc
     2161 cgatgttgcc ggaacgcagc tccgggaagg cgacggtccc ggccgcgcgg cctttctggg
     2221 tcatgcccgc ctcatgggcg cgggtagccg cgaccgtctg tttcaggtcg cggttccaga
     2281 gtaaggcgtt catcgccagg tccagatgtc cgtcgataat cagcacagtc tcctccttct
     2341 gctctcgctc agactcacat ggccaggtag gcggaacgta cccgcatctg tttcacctgt
     2401 caacaccagt gttgacaggt gatcacagga tgggtagatt ggccgagtac agcaaagcaa
     2461 catcccacgg caacgaactg gccgcgattc cagtttccac ggagaggagg ggccgccaca
     2521 ttcgcgacgt cgttgccgcg tatcagcaat cccgcaacgc gcgaaccgtg cttgtggttc
     2581 gcaccggagt ctggagcctg agggggagaa caacaggtgg aagacaatcg caacgttcag
     2641 aagttactcg acgacattac ccggcacggc ctgagccgcc gcgcggtcct caagcgggcc
     2701 gtcgcgcttg gtctggccgc gcctaccatc tcgatgctgc tggccgcctg tggcggtgaa
     2761 gatgacgacg acgatgatgg cggcgattcg ccaacggcaa cgtcagcatc gggtgcggca
     2821 acgacaccag ccgaggcgac ggaagcgccg gaggaaacgc cggaagccga ggcgaccgaa
     2881 gcgtccgccg agacaccggc ggcaggcgag actccggcag gtgagacgcc ggccgcgggc
     2941 gaaacaccag ctgccggtga gactccggcg acatcggccg agaacgacat ctgggccgat
     3001 ggcaaggcgg gtggcaggct gaagatcgcg tcgatcggcg aaccggccac gctcgacgag
     3061 catcagacga ccgccggcat tacggccgaa atcggctact gcatgtacga gacgctcttc
     3121 gcctacgacg cgaactacct gccagtgccg atgctggctg agtcgtacga tgtcagcgac
     3181 gacggcaccg tccacacgtt caagcttcgc cagggcgtgc cattccacaa cggcgaggag
     3241 atgacctccg ccgatgtcgt cgcttcggtc gagcgctggg gccgaatcag cggcgtcggc
     3301 aagaacgtga tggcggcgac tacgagcatc gaggcgaccg acgattacac cgtcacctgg
     3361 acgacgacca agccatacgg cacgatcctg atcgccttgt cgaacaacac ccagggctgc
     3421 gtgatctacc cgaagtcgct gctggatacg cagtctgatg acccgatcgc gtcggccgac
     3481 atcgtcggga ccggccccta caagctcgtc gagcatcagc cggatgttca catcctgatg
     3541 gagcgcttcg aggattacgc cgcgctggaa ggtggcgcac tcggcagtta cggcggccac
     3601 aagtacgcct gggtcgatga gatcgagttc gtcccggtgc cagatgaggc gtcgcgggtg
     3661 gccggcatgc aggccgggga ttaccacatc ggcgcaacga tcgggaacga ccactacgac
     3721 gtcctcaaag gcagcggaga tctgctggtc gagattctgc caccgagcca gtgggatgtc
     3781 ttcttcctca actgggcgca gccgatgttc gccgacgaga acaaccgcaa ggcgttccag
     3841 gcctgcctcg atcacctgcc gatcctgcag aactcgcgcg gtggtggcga cttcgtcgcg
     3901 ctcgacccca gtctgatgca gaagccgagt gcgttctact cgacggccgg aggcgagctc
     3961 tacaacatcc atgatgtcga gctggccaag cagtacctgg ccgacagcgg ctacgacggt
     4021 acgccggtgc gcttcatgtg cactcaggaa tatgcctata tgtacggcgc ggccatcatc
     4081 gctcagcagc agatggaaga ggccggcttc gtcgtcgacc tgcaggtcac cgactgggcg
     4141 accgtcctcg aacgacgcgc caagaaggaa gagtgggaca tcttcgtcac cggccacggc
     4201 ttcgtgccgg acccgaccca gatcacctac atcggccaga tgaacatcta ccccggctgg
     4261 tggaacgacc caacggcact ggacctcgcc gcccagctgg ccagcgagac ggagttcgag
     4321 aagcgctacg agatctacga acagatccag cagttggcct acgagcaggt gccggccgtc
     4381 aagaccggtg actcaagcac gatcgtggtc tgggcaggca gcgtcggcgg ctacggggct
     4441 ccgatccagc gcggcgtgcc gtactggaac gtctggctcg acgaatagct cacttcggcc
     4501 ggccgtcagc gctcggctga cggccggcgg ccaagggact ctctatgctg acgtatgtca
     4561 ttcgccggct gatcctgctg ataccggtga tcgcgatcgt cgggacgatc gtgtttgtcc
     4621 tgatccacct gacgccgggc gacccggcgg ccgtcatcct cgggccggac gcgacgcccg
     4681 agcagatcga gtcgttgcgc gaccggctcg gcctgaacga gccgctctat gagcagtatg
     4741 tccactggtt cgccggcgcc gtccggctcg actttggcga ttcgatcttc cttggccagc
     4801 cggtgacgca gtcgctgctg gaccgcgccc agccgacact gctgctgacg ctgtatgccc
     4861 tgacgatcca ggtcctgatc gcgatcccgt tcggcgtcat ctcggccgtc aagcacaact
     4921 cgctcctcga ccggatctta atggtgctct cgatcagcgg tgcggcgatc cccagcttct
     4981 tcctggggat catgctgatt ctgttcttcg ccgtccggct gcagtggctg ccgtcgggag
     5041 ggttcacgtc gatcacggaa gatccagttc agcacttcaa gcagatgatc ctgcctgcct
     5101 tcgcgctcgg cttcacgtcg gccgggctgc tggcgcggct ggtacgttcg tcgatgctgg
     5161 atgtcctcaa ggaggactat atccgaacag cgcaggccaa agggctgcca cagcggatga
     5221 tggtcgtccg ccacgcgctg cgcaacgcgc tgattccggc tatcacggtc attggctact
     5281 cgctcggcgg cctgctgggc ggcgcggtcg tgaccgagac ggtcttcacg atccccggta
     5341 tgggccggct ggtcgtccag tcagttgccc ggcgcgactt cccggtcatc cagggggcga
     5401 tcctgatcat cgcggcaatc tacgtcctcg cgaatctgct cgttgacgtg ctctacgtct
     5461 atatcgatcc acgggtgcgc tatggcaacg actgaacaca cggagaccgc tccgctcgca
     5521 aacgccctct cgacgcggcc tgtccagcgc ggcctgctgc gcaatatcac gccggcctca
     5581 cgcttctact gggtacgggt gctgctgacc aacccgatgg cggtcgccag cgggacgatc
     5641 ctgctcatca tcatcctcgc cgcgatcgcc gctccagttc tgacgagcca cgatcccaag
     5701 ttcctcgacc ccggtatccg acttcaaggg ctgactggag agcactggtt tggcaccgac
     5761 ggcagcgggc gcgatgtctt cgcgcgcgtc ctctatggcg cgcgcgtctc gctgatcgtc
     5821 gggctatcgg tgatggcgat ggcggcgtcg atcggcgcgg tcctcgggtt gatctccggc
     5881 tactaccagc ggctcgacac gccgatcatg cgtgtcatgg atggtctgat gtcgttcccc
     5941 agcattctgc tggcgatcgc gatcatggcc agcctcggcc caagcacgat caacgtcatc
     6001 atggcgctga cggtggtcta catccccggc gtcgcccggc tggtgcgcgg gacgacgctg
     6061 gtgacgaagc agcaggcgta cgtcgagtcg gcacgggcag tcggtgtccg cgaccacgtc
     6121 atcctgcggc gctacatctt cgccaacacg ctctcgccgc tgatcgtcca gtgcaccttc
     6181 gtggtcgcgt tcgcgatcat ctccgaggcg tcgctctcgt tcctcggcgc cggcgtccca
     6241 cccgagacac ccacctgggg caacatgctg cgcgacggac agcagttcat ccagatcgcc
     6301 tggtggatgg ccgtcttccc cggcagcgcg ctcttcatcc tcgtcctcgc gctcaacctg
     6361 ttcggcgacg gcctccgcga cgcgctcgat ccgcggatgc ggagacggta ggcgggttcg
     6421 ctggacgcgg gacgcgggac gcgggcagga tagaaccaca tccaaccgga acgccaggaa
     6481 gtaggggccg tgccttgtgc cggccctgtt ccccacccga ggcgttcggg aacccgagca
     6541 gggccggcac aagaccggcc cctgcttcct agcgaacgag cgttccatcc gcgtcacgcg
     6601 ttacgcatca cgcgtcccgg catcactgca cccatatttc gcaattcacg aacgtgtcgg
     6661 tgctgttgcc gccatagcag atatcaaagc ctgggccgcc gtcgaggtgg tcggcactgt
     6721 tatctccaaa gaggatgtcg tcaccagggc cgccaagcag aatgtcactc tggttgccac
     6781 cgtgcagaat gtcaatccca ccaagcccga ccacgcagtc gcggccattg ccgccgaaga
     6841 ggatgtcggg accgcttgtc cccatgatga cgttgttgcc ggtgatcgtg ccgcagtcgc
     6901 cttcctgctc cggtggctcg aaggcgagcg tctggtcgtc ggcagaggat gggtcgaccg
     6961 tgttggcagc ggccagcgcg ccgaacgaga cccagaccag cagcgtgcag gcggccagca
     7021 tcagcagccg ccgcttcgtc aggcggatca ctggaccacc ccgggagccg ccgagtacag
     7081 gtaggtgatg tcggcgcaac ggacgataaa gcgctcgaac ccgttggcgg cgtggcgcag
     7141 gatgacctgg ccatcccgct cggccagccg ggcgaggtcg tcgaacgagc
//
LOCUS       DASUGJ010000159         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1271347, whole genome shotgun sequence.
ACCESSION   DASUGJ010000159 DASUGJ010000000
VERSION     DASUGJ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271347"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2103)
                     /locus_tag="VFV93_03425"
     CDS             complement(<1..2103)
                     /locus_tag="VFV93_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5164424.1"
                     /translation="MTDDKTLAGERLLHRLSTALAREAWPDAVVRVALDALVSEVGAR
                     SAGVFIVDERTSTVSPVYSVNYLPDMLDQIRETSLESPALSNLAIRTGEVQVIPSIDE
                     VPEGVTFTKQLSDRIGVKAVAAVPLLLGGRTLGVLVYGLAEEHQFDDAELRLLREVGE
                     RITTAMERGRLEEALARRADEAELLHSIALAAAGEDDLNRILNAALARLAGLLNFTGG
                     SIALVEGDELVVCAASGVLAGATLNQRVSRDDGTVWQVLATGTPFLSNELPVAQSPLP
                     DEDARRAVRSLLVVPLVWRGAPFGVLQVDSIRPNAFQESDIGLMQRVATLLSGSIELA
                     RRYAAEVQLRRDLDQARGRLAAIIQHAPMGISFFDADDQLVFANQSASETLHLYPEHD
                     GPPGRSWEDMIEQVLRWRWAGDPDTLRQIVAETRESREGILVHDFPLRTPEQMLLRIA
                     APVTESGRFSGHVILLIDVTSERQALAAAEQAIALRDRFISIASHELKTPLTAIKGIA
                     QLLRRAHATDRLDLVRLGDQLTTINIQAERLQELIDDLLDISRIQSGRLELRREPVEL
                     VAIVDGLIAAMPDERRRRVRLDAAQPVHGNWDPMRLEQVAQNLLDNALKYSAPDTTVD
                     VLIRTDGTDALLSFSDCGIGIPAGEIATLFEPFARAANAGLRDDSGLGLGLYITRQIV
                     ERHGGTISVESVERHGGTI"
     gene            complement(2181..3083)
                     /locus_tag="VFV93_03430"
     CDS             complement(2181..3083)
                     /locus_tag="VFV93_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX5164425.1"
                     /translation="MNSVSASRVPRPASRTPSPLLLVISLIGAASFLYPFFLPVLAGV
                     GARTARGGIEVPLVFTALGLACLLVILLEVQGRAGIGDQGASKLVALLGMLVAVDATL
                     RLVPSLLGASPIFLLIVLVGFAYGAEIGFLMGALTLFVSAIITGGLGPWLPYQMLGAG
                     WVGMTAGWLPRSGEPGGRRELVLLALFGGAWGFLYGAILNLMEWPYAAPGLSERAGLY
                     WLPGMGFGETVATYLRFYVTSSLVYDAFRAGANAVLVLALGRPLLRLLDRYRDRFTWE
                     PWVSRATDDISGVTSGGHSNYHAR"
BASE COUNT          525 a         1083 c         1076 g          440 t
ORIGIN      
        1 gatcgtgccg ccgtggcgtt cgacgctctc gacgctgatc gtgccgccgt ggcgttcgac
       61 gatctggcgg gtaatgtaga ggccgaggcc gaggccgctg tcgtcgcgca agccggcgtt
      121 ggcggcgcgg gcgaacggct cgaacagcgt cgcgatctcg ccggccggga tgccgatgcc
      181 gcagtcgctg aaggacagca gcgcgtcggt gccgtcggtc cggatcagga catcgaccgt
      241 cgtatcgggc gcggagtact tgagggcgtt gtcgagcagg ttctgggcga cctgttcgag
      301 gcgcattggg tcccaattgc cgtggacggg ctgggcggcg tcgagacgga cgcggcggcg
      361 gcgctcgtcc ggcatcgccg cgatcagacc gtcgacgatc gcgaccagtt cgaccggctc
      421 gcggcgtagt tccagccggc cggactggat ccgcgagatg tcgagcaggt cgtcgatcag
      481 ttcctggagc cgttcggcct ggatgttgat cgtcgtcagt tggtcgccga gccggacaag
      541 gtcgagccgg tcggtggcat gggcgcggcg cagcagctgg gcgatgccct tgatcgccgt
      601 cagcggcgtc ttgagctcgt gcgaggcgat cgagatgaag cggtcccgca gggcgatcgc
      661 ttgctcggcc gccgccagcg cctgccgttc gctggtgaca tcgatcagca ggatgacatg
      721 gccggagaag cggcccgact cggtcaccgg cgcggcgatc cgcagcagca tctgctccgg
      781 cgtacgcagc gggaagtcgt ggacgaggat gccctcacgc gactcgcgtg tctcggcgac
      841 gatctgccgc agcgtgtccg ggtcacccgc ccagcgccag cgcaggacct gctcgatcat
      901 gtcctcccag gagcggcccg gtggcccgtc gtgctccggg tagaggtgca gcgtctcgga
      961 tgccgactgg ttcgcgaaga ccagttgatc gtcggcatcg aagaaggaga tgcccatcgg
     1021 cgcatgctgg atgatcgccg ccagccgccc gcgcgcctgg tcgaggtcgc gccggagctg
     1081 cacctcggcg gcgtagcgac gcgccagctc gatcgagccg ctcagcaacg tcgccacccg
     1141 ctgcatcagg ccaatgtccg actcctggaa ggcgttcggg cggatcgaat cgacctggag
     1201 gacgccgaac ggcgctcccc gccagaccag cggcacgaca agcagcgagc gaacagcccg
     1261 tcgcgcgtcc tcgtctggca ggggcgactg cgcaaccggc agttcgttgc tgaggaaggg
     1321 agttccggtt gccagcacct gccagacggt gccgtcgtca cgcgatacgc gctggttgag
     1381 ggtggctccg gccaggacac cactggcggc gcagacgaca agctcgtcac cctcgaccag
     1441 cgcgatcgag ccgccggtga agttcagcaa cccggccagc cgggccagcg ccgcgttgag
     1501 gatgcggttg aggtcgtcct cgccggccgc ggccagcgcg atcgagtgca gcagctcggc
     1561 ctcgtcggcg cggcgggcca gcgcctcctc cagccggccc cgctccatcg cggtcgtgat
     1621 ccgctcgccg acctcgcgta gcagccgtag ctcggcatcg tcgaactggt gctcttcggc
     1681 cagcccgtag acgagcaccc ccagtgtcct gccccccagc agcagcggga cggcggccac
     1741 cgccttgaca ccgatccggt cggagagttg cttggtgaag gtgacgccct cgggaacctc
     1801 gtcgatcgac gggatgacct ggacttcgcc ggtccggatc gccaggttgg agagggcggg
     1861 actctcgagc gaggtctcgc gaatctggtc gagcatgtcc ggcaggtagt tgacgctgta
     1921 gacggggcta accgtcgagg tgcgttcatc gacgatgaag acgccggcgc tgcgagcgcc
     1981 aacctcggag acgagcgcgt cgagcgcgac acgcacgacg gcatccggcc aggcctcacg
     2041 cgccagcgcg gtactgagcc ggtgcaacag gcgctcacca gccagtgttt tgtcatcggt
     2101 cacgttgcgt ccgcgctccg tgacacacgg ccaccgttca tcgctctccc aaccggaccc
     2161 ccaccggtcg ctcgcttccc ctaacgagca tgatagttgg aatgaccacc ggatgtcacg
     2221 cctgagatgt cgtctgtcgc ccggctgacc cacggctccc aggtgaagcg gtcacggtag
     2281 cggtcgagca gccgcagtaa tggccggccg agggcgagga cgaggacggc gttcgcgccg
     2341 gcccggaagg cgtcgtagac gagcgaactg gtgacgtaga agcgcagata ggtcgcgacc
     2401 gtctcgccga agcccatccc cggcagccag tagagcccgg cccgttcgct caggcccggc
     2461 gcggcatacg gccactccat cagattcagg atcgcgccgt agaggaaccc ccacgccccg
     2521 ccgaacagcg ccagcagcac cagttcgcgc cgcccgcccg gctcgccgga tcgcggcagc
     2581 cagccggccg tcatcccgac ccagccggcc cccagcatct ggtacggcag ccacggcccc
     2641 agcccgccgg tgatgatggc cgagacaaac aacgtcagcg cgcccatcag gaagccgatc
     2701 tcggcgccat aggcgaagcc aaccagcacg atcagcagga agatcggtga agcgcccagc
     2761 agcgacggca ccagccgcaa ggtcgcatcg acggcgacca gcatccccag cagcgcgacc
     2821 agcttcgacg ccccctggtc gccaatcccg gcccgcccct gcacctccag caggatcacc
     2881 agcaggcagg ccagccccag cgccgtaaag accagcggca cctcgatccc accccgcgcc
     2941 gtccgcgccc caacacccgc cagcaccggc agaaagaacg gatacaggaa cgacgccgcg
     3001 ccgatcaggc tgatgacgag gaggaggggg gaaggtgttc gggacgcggg acgcgggacg
     3061 cgggacgcgg atacgctgtt catcgggttt actccgcgtc ccgacgttcg tccggcgcga
     3121 agat
//
LOCUS       DASUGJ010000160         8346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_855588, whole genome shotgun sequence.
ACCESSION   DASUGJ010000160 DASUGJ010000000
VERSION     DASUGJ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855588"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VFV93_03435"
     CDS             complement(<1..909)
                     /locus_tag="VFV93_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164426.1"
                     /translation="MLNALHVPPEPERATPGDLRDLLMRLDWRQELLSILLVLAETAL
                     IYIFVGFALPEAEPGSAVAPVWIVVGLMLVAHLVPHLLDEWRVWSPNYEIVLASAIAI
                     SALVAIKVASFPDIALWDLDWLRGTVNALALLDNDAARPVWGIVVLSAYAWWRGRTRA
                     EGSIDSAYAMLRTGTLAMALLLILILVAAPDDAQVRDRLSTATIAFFASALAAIGVAR
                     LTLEGFRTSAPLGPRWLATFVGPILAVVAVAIIGAGIFSRQFLDTVLWMLTPVFFVLN
                     LVFQVFVFVLAIIAFIILSPLVWLIGN"
     gene            complement(914..2266)
                     /locus_tag="VFV93_03440"
     CDS             complement(914..2266)
                     /locus_tag="VFV93_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5164427.1"
                     /translation="MVHVQHQPSADDQAASEGSTSQARSLFFNSIWLWGSGLLILLGA
                     ALSERSLALLGLLTLVTAGGAWLWARFALNHIAYTRTLATDRIFPGEQVAMRVSLVNR
                     KLLPLAWLDIEDQIPDRLRIVEQEVIPSGIPGMETLRITTAMRWYERVSWRFHVECPV
                     RGYFTLGPVGLRSGDLFGFFNAREDLESYASIIVYPRVVPLDEIGIPPRHLFGDLRIR
                     RQIITDPSRAVGVREYRPEDSFRYVHWKATARTQELQTKVFEPTTAVQFGVFLNLDTF
                     EHYWEGIDYQRAENAITVAASLAIHALDTRHTVGMYANGVVGGSDQPLRVRPSRSPEQ
                     AHLVLSGLAKLSPIASLNFPRLLRTETLRFPWGSTIVVVTALMSDPLAAAMAELARAG
                     HRLVLVTIDEIATPPIRGLLTFKIDSDQVAGPRPPRRRYARQLHPIHHPVTAAVVERD
                     "
     gene            complement(2292..3257)
                     /locus_tag="VFV93_03445"
     CDS             complement(2292..3257)
                     /locus_tag="VFV93_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5164428.1"
                     /translation="MQTTGSTTGEIAAFSNRIIDNVDRVIVGKRDVTELVLVALLCEG
                     HVLLEDVPGVGKTTMARAVARSIGGEFKRIQFTPDLLPSDISGISYYNQKQGDFLFRA
                     GPIFTNILLADEINRATPRTQSALLEAMEERTVTIEGDTMALPRPFLVLATENPVELE
                     GTFPLPEAQLDRFLLRITIGYPDADQEDEMLVRLQHQHPLGSLEPVVTPDDLLAASQG
                     VRAVHVEPELRRYLASVTRATREHEAVDLGVSPRGTLALFRAPQAIAALRGRDFATPD
                     DTKLVAPSVLNHRMLLSPEARLRGRSVPAILNEILDSLSAPVERV"
     gene            3379..5622
                     /locus_tag="VFV93_03450"
     CDS             3379..5622
                     /locus_tag="VFV93_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5164429.1"
                     /translation="MSTYNGDSKGDGVAERPPRQYSIYHRLLTGLSAITAIVLALVIG
                     LNVAVRTEINHRSTILAATIGSLVILGLALWVTIVLLRALRELQATVGEVTEAVNRGE
                     GERRVPRLTAREFDDLGQSVNQMLDSLSANIAEANLQRQRTAAIVDSASEGIVVVDAA
                     GVVTNINPAAARMFETTIDEALSMPAVDLGFFSEQEIGEIFEQPQDGSVRPVVRRRGD
                     RVLSAAVATLQSNAGEPSSGLVWVLRDVTELAQIDEMKSEFISIVSHELRTPLTAIKG
                     FTDLIIEGEVGEISEQQREFLEIVQSNSDRLVALINDMLDISRIESGRITLNLEGVDV
                     PAAVEHAVVALRPLVEDKQLQIQTELVEDPATIIADRTRLQQILTNLISNACKYTLPG
                     GWITVRSEALDGQIALSVSDTGIGIPPEALPHIFSKFYRVDQPQTRDVGGTGLGLAIT
                     KSLVEMHGGRIQIASRAGLGTTVRFTLPVAGPYDNGGPTPADNSTHSGLVLVVTDDSV
                     EREAWESAIRRVPADVVVARGTSAAAAIGEAELHRPSVIVARCGDGRSTLDLEELIEE
                     LQASVELRQTPVVAVVPNVETCPSPTEGIVLPATASPTEVAEAVSGLLPESAERRPQR
                     GRVLVAEDDGDTANWLRRVLVRNGFEVVLVRDGLAAIVRAVEILPDAIVLDVNMPRMG
                     AGEVLPQLHSNPGTREIPVIVISGTVPDSGPYFIDAGADDFFSKPFNGDLLVRRLIQL
                     TRKRQDG"
     gene            5615..5995
                     /locus_tag="VFV93_03455"
     CDS             5615..5995
                     /locus_tag="VFV93_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5164430.1"
                     /translation="MASVVVIEDEPHIATLVAVKFRNAGHQVEIATDGEAGLDLVLRR
                     EPEVVLLDVMMPKMDGFEVCQAIREHFDETQRPTVVLLSARSQLADRQRGIDAGCDDY
                     IVKPFRPAALLDRVTELLKERESQ"
     gene            complement(6028..6708)
                     /locus_tag="VFV93_03460"
     CDS             complement(6028..6708)
                     /locus_tag="VFV93_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5164431.1"
                     /translation="MEAAGKPITARRLARMRGTLARRQPDLAVVMENIYDGHNVSAML
                     RSCDAVGAATAHLVYDVEEVPEIAGGISVSAHRWLDLRTHASIGDCFAELRAAGIAIY
                     ATTLGEFSHDLYDLDLTQPTAFVFGNEVRGVTTEAAAASDGTVFIPMMGMVESLNVSV
                     ACAVCLYETLRQRQLAGRYGQASWPAEEIDARLRGWLEREGRDPAAAAQAIETTVPRA
                     RNRHEQRG"
     gene            6804..7403
                     /locus_tag="VFV93_03465"
     CDS             6804..7403
                     /locus_tag="VFV93_03465"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5164432.1"
                     /translation="MVRAINTDHRLTFEEYLAFEETSDVRHELVDGHLYAFAGASDRH
                     NLICSNLIGTLWMHARGSSCRVFGSDMKVRVSSRVGYYPDVMVACDPEDNHSLYRSRP
                     CLLVEVLSPSTRLVDRREKLIAYQSIASLRGYLMVYRDEYRVEAYTRNGDDVWVYANL
                     TTDDNVWFPCIDVTVPVMALYEDVDMSPIVPTAEEPDIR"
     gene            7554..7721
                     /gene="rpmH"
                     /locus_tag="VFV93_03470"
     CDS             7554..7721
                     /gene="rpmH"
                     /locus_tag="VFV93_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX5164433.1"
                     /translation="MPKRTYQPKRLHRKKDHGFMKRMSTRAGRAVLAARRRKGRHSLS
                     VSTEYKPTQKK"
     gene            7764..8150
                     /gene="rnpA"
                     /locus_tag="VFV93_03475"
     CDS             7764..8150
                     /gene="rnpA"
                     /locus_tag="VFV93_03475"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX5164434.1"
                     /translation="MGPGIWPELLPMVARELRLRRGSDIDRVRSKGRSWSSRLLVLAI
                     LPNGLEHNRYGFTVGKRVGGSVERNRAKRLMREAIRHCHPTLVQGHDVLLIARNSFRD
                     ETTLAEVAEQLDGLLRRAGIRSAETG"
     gene            8147..>8346
                     /gene="yidD"
                     /locus_tag="VFV93_03480"
     CDS             8147..>8346
                     /gene="yidD"
                     /locus_tag="VFV93_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX5164435.1"
                     /translation="MRRLALLLIRGYQRGISPMLPPSCRFVPTCSDYGYQAIEKYGII
                     RGGAMTVWRIMRCNPFNKGGFDP"
BASE COUNT         1537 a         2657 c         2774 g         1378 t
ORIGIN      
        1 gttgccaatc aaccagacaa gcggcgagag gatgatgaag gcgatgatgg cgagaacgaa
       61 gacgaacacc tgaaagacga ggttgagcac gaagaagacc ggcgtcagca tccagaggac
      121 ggtgtcgagg aactggcgcg agaagatgcc agcgccgatg atcgcgacgg cgacgacggc
      181 caggatcggc ccaacgaagg tcgccagcca gcgcgggcca agcggtgcgc tggttcgaaa
      241 tccctccaac gtcagtcgcg cgacgccgat cgcggccagc gcgctggcga agaaggcgat
      301 cgtcgccgta ctgagccggt cgcgaacctg ggcatcatcg ggcgcggcga ccagaatcag
      361 gatcagcaac agcgccatcg ccagcgtccc ggtccgcagc atggcgtagg cgctgtcgat
      421 cgagccttcg gcccgcgtcc ggccccgcca ccatgcgtag gcggacagga cgacgattcc
      481 ccagaccggt cgagcggcgt cgttgtccag caacgccagc gcgttgacgg tgccgcgcag
      541 ccagtcgaga tcccagagcg cgatgtctgg aaaactggcc actttgatcg ccaccagagc
      601 cgagatcgcg atcgcggagg cgagcacgat ctcgtagttc ggactccaga cgcgccactc
      661 gtcgagcaaa tgcggcacga gatgggccac cagcatcagc ccgacgacga tccagaccgg
      721 cgcgacggct gaccccggct ccgcctccgg cagcgcgaac ccgacgaaga tatagatcag
      781 cgccgtctcc gccagcacca gcaggatcga cagcagctcc tgccgccagt cgagccgcat
      841 cagcaagtcg cgcagatcgc ccggcgtggc gcgctccggt tcgggtggga cgtgcaacgc
      901 gttcagcaac ggctcagtcc ctctccacga cggctgccgt cacagggtgg tggatcgggt
      961 ggagctggcg cgcgtaccgc cgccggggcg ggcgcgggcc ggcgacctgg tcgctgtcga
     1021 tcttaaaggt cagcaggcca cggatcggcg gcgtcgcgat ctcgtcgatc gtgaccagca
     1081 cgagccggtg gccggcacgg gccagttccg ccatcgcggc agcaagcgga tcgctcatca
     1141 gcgctgtgac gacgacgatt gtgctgcccc agggaaagcg cagggtttcg gtgcgcagca
     1201 ggcgcgggaa gttgagcgac gcgatcggcg agagtttcgc cagccccgac aggaccaggt
     1261 gggcctgctc cggactgcgg ctgggccgca cccgcagcgg ctgatcggag ccgccgacga
     1321 cgccgttggc gtacatcccg accgtgtgcc gcgtgtcgag cgcgtgaatc gcgagcgagg
     1381 cggcgaccgt gattgcgttc tctgcccgct ggtagtcgat gccctcccag tagtgctcga
     1441 acgtgtcgag gttgaggaag acgccgaact ggacagccgt cgtcggctca aagaccttgg
     1501 tctgcaactc ctgggtacgg gccgtcgcct tccagtgcac gtaacggaac gaatcttcag
     1561 gccggtactc gcggacaccg accgcccgcg acggatcggt gatgatctgg cgccggatcc
     1621 tcaggtcgcc gaacaggtga cggggcggga tgccgatctc gtccagcggg acgacgcgcg
     1681 ggtagacgat gatcgaggca tagctctcaa ggtcctcgcg ggcgttgaag aagccgaaca
     1741 gatcgcccga gcgcaggcca accggcccga gcgtgaagta gccccggacc gggcattcga
     1801 cgtgaaagcg ccacgagacc cgctcgtacc agcgcatggc ggtcgtgatc cgcagcgtct
     1861 ccatgccggg gatgccactg ggaatcactt cctgttcgac gatccgcagc cggtccggga
     1921 tctgatcctc gatgtcgagc caggccagcg gcagcagctt gcggttgacg aggctgaccc
     1981 gcatggcgac ctgctcgccg gggaagatcc ggtcggtcgc gagcgtgcga gtgtaggcga
     2041 tgtggttgag cgcgaagcgt gcccagagcc acgctccacc ggccgtgacc agcgtcagca
     2101 gcccgagcaa cgccagcgag cgttccgaga gggcagcgcc gagcaggatc agcagcccag
     2161 aaccccagag ccagatgctg ttgaagaaga gtgagcgtgc ctggctggtg ctgccttcgg
     2221 aggcagcctg atcgtcggct gacggctggt gctggacatg aaccatcggc ggccgacctt
     2281 ctccgtgcgg gctagacgcg ctcgaccggg gcgctgaggc tgtccaggat ctcgttcagg
     2341 atggccggga cgctgcggcc ccgcagccgc gcctccggcg agagcagcat ccggtgattg
     2401 agaacggatg gcgccaccag cttggtgtcg tccggcgtcg cgaaatcacg tccgcgaagg
     2461 gcggcgatcg cctgcggtgc ccggaacagc gccagcgttc cgcgcgggct gacgccgaga
     2521 tcgaccgcct cgtgctcgcg cgtggcacgc gtcacgctcg ccaggtagcg ccgcagctcc
     2581 ggctcgacgt ggacggctcg cacgccctgg ctggcggcca gcaggtcgtc cggcgtcacg
     2641 accggttcga ggctgccgag cggatgctga tgctgaagtc ggaccagcat ctcgtcctcc
     2701 tgatcggcgt cgggatagcc aatcgtgatt cgcagcagga agcggtcgag ttgcgcctcc
     2761 ggtagcggga acgtgccttc cagctcgacc gggttttcgg tcgccaggac gaggaaggga
     2821 cgcggcagtg ccatcgtgtc gccctcgatc gtgaccgtgc gctcctccat cgcctccagc
     2881 aacgccgact gcgtgcgcgg cgtcgcccgg ttgatctcat ctgccagcag aatgttggtg
     2941 aagatgggtc cggcccggaa gaggaaatcg ccctgcttct ggttgtagta ggagataccg
     3001 gagatatcgg acggcaagag atcaggcgtg aactggatgc gcttgaactc gccgccgatc
     3061 gagcgggcga cggcccgcgc catcgtcgtc ttgccgacgc cggggacatc ctcaagcagg
     3121 acatgcccct cgcagagcaa cgccaccagc accagttcgg tgacgtcgcg cttgccgacg
     3181 atcacccgat cgacgttgtc gatgattcgg ttactgaacg cggcaatctc gccggtcgtc
     3241 gatccggtcg tctgcatact cgtgactacg ccgatctgct ggaaggcgga tgtatcaatt
     3301 gttgatgacg gttcaaatgc tgcaaaaaac tataccaccg gctatcctga ctgccggtgg
     3361 cgcggcatga aagcggcgat gtccacctac aatggcgact cgaaagggga cggcgtggcc
     3421 gagcggcccc ctcgacaata cagcatctac caccggctcc tgacgggtct cagcgcgatt
     3481 accgcgatcg tgctggcgct ggtgatcggg ctgaacgtcg cggtccggac cgaaatcaac
     3541 catcgatcca cgattctggc agcgacaatt ggcagcctcg tcatcctcgg cctggcactc
     3601 tgggtcacca ttgtcctgct gcgagcgttg cgcgagctac aggcgaccgt cggcgaagtc
     3661 acggaagccg tcaaccgtgg cgagggggag cgacgtgtac cgcggttgac ggccagagag
     3721 ttcgatgacc tcggccagag cgtcaaccag atgctcgatt ccctctcggc caacatcgcc
     3781 gaggcgaacc tccagcggca acgtacggcg gccatcgtcg atagcgccag cgagggcatc
     3841 gtcgtcgtcg acgcggctgg cgtcgtcacc aacatcaatc ccgctgccgc gcggatgttc
     3901 gagaccacca tcgacgaggc gctgagcatg ccggctgtcg atctgggctt cttctcggag
     3961 caggagatcg gcgaaatctt cgagcagccg caggacgggt cggtccggcc agtcgttcgc
     4021 cggcgcgggg atcgtgttct cagcgccgcc gttgcgacgt tacaaagcaa tgccggggaa
     4081 ccgtcgtccg ggctcgtctg ggtgctgcgc gatgtcaccg agctggcgca gatcgatgag
     4141 atgaagagcg agttcatctc gattgtgtca catgagctac ggacaccgct gacggcgatc
     4201 aagggtttca ctgacctgat catcgagggc gaagtcggcg agatcagcga gcagcagcgc
     4261 gagttcctcg agatcgtcca gtccaacagt gaccggctgg tcgcgctgat caacgacatg
     4321 ctcgacatct cccggatcga gtccgggcgc atcacactca atctggaagg cgtcgatgtc
     4381 ccggccgccg tcgagcatgc ggtcgtcgcc ctacggccgc tggtcgagga caagcagcta
     4441 cagattcaga ccgagctggt cgaagatccg gcaacgatca tcgccgatcg aacccgctta
     4501 cagcagatcc tgacgaacct gatttcgaac gcctgtaagt acacgctgcc gggcggctgg
     4561 ataacggtcc gcagcgaggc gttggacggg caaatcgcgc taagtgtctc cgacaccggt
     4621 atcggcatcc cgcccgaggc gctgccacac atcttctcga agttctaccg ggtcgatcag
     4681 ccacagaccc gcgacgttgg cggaaccggc ctgggcctcg cgatcacgaa gtcgctggtc
     4741 gagatgcacg gcggcaggat ccagatcgcc agccgggccg gcctcgggac aactgtccgc
     4801 ttcacgctgc cggtcgccgg gccgtacgac aacggcggtc cgacgccggc tgacaacagc
     4861 acgcacagcg gcctcgtcct ggtcgtgact gacgacagcg tcgaacgtga ggcgtgggaa
     4921 tcggcgattc gccgcgtccc tgccgatgtc gtcgtcgccc gtggcacatc ggccgccgcc
     4981 gcgatcggcg aagcggagct gcatcgcccc agcgtcatcg tggcgcgctg cggcgatgga
     5041 cgcagcacgc tcgacctgga ggaactgatc gaagagctgc aagccagcgt cgagttgcga
     5101 caaacgccgg ttgtcgcggt cgtgccgaac gtcgagacgt gtccatcacc caccgaaggg
     5161 atcgtgcttc ccgcgacggc gtctccgacc gaggtcgccg aggcggtcag cgggttgcta
     5221 cccgaatcgg ccgagcggcg accccagcgg gggcgggtgc tggtcgccga agacgacggc
     5281 gacaccgcaa actggctgcg ccgggtcctc gtccgcaatg ggttcgaggt cgtgctggtg
     5341 cgcgacgggc tggccgccat cgtccgcgca gtcgagatcc tgccggacgc aatcgtgctg
     5401 gacgtcaaca tgccgcgaat gggggccggc gaggtgctgc cacagctgca cagcaacccg
     5461 ggcacccggg agataccggt catcgtcatc tctggcaccg tcccggactc cggtccgtac
     5521 ttcatcgacg ccggggcaga cgacttcttc agcaagccgt tcaacggcga tctgctggtc
     5581 cggcggctga tccagctcac caggaagagg caggatggct agcgtcgtcg tcatcgaaga
     5641 cgagccgcac atcgccacgc tggtcgcggt caagtttcgc aacgccggtc atcaggttga
     5701 aattgcgacg gatggtgaag ccggcctcga tctggttctg cggcgcgaac ccgaggtggt
     5761 cctcctggac gtcatgatgc cgaagatgga tggcttcgag gtctgccagg cgatccgcga
     5821 gcacttcgat gagacgcagc gtccgaccgt cgtcctgctg tcggcccgta gccagctcgc
     5881 cgaccgccag cgcggcatcg acgcgggctg cgacgactac atcgtcaaac cgttccggcc
     5941 ggcagcattg ctggaccgcg tcacggagtt gcttaaggag cgggagtcgc agtgagcgag
     6001 tggggtgccc tggacgacag cagggtatca gccgcgctgc tcgtggcggt tgcgcgctcg
     6061 cgggacggtt gtctcgatcg cctgcgcggc cgccgccgga tcgcggcctt cacgctccag
     6121 ccaaccgcgc aggcgtgcgt cgatctcctc cgcaggccag ctcgcctgcc cgtaccggcc
     6181 ggccagctgg cgttgccgga gcgtctcgta cagacaaacg gcgcaagcga ccgagacatt
     6241 cagcgactcg accatcccca tcattgggat gaagaccgtt ccgtcggatg cggcagccgc
     6301 ctcggtcgtc acaccgcgca cctcgttgcc gaagacgaac gctgtcggct gagtcaggtc
     6361 caggtcgtag aggtcgtggg agaactcgcc aagcgtcgtc gcgtagatcg cgatcccggc
     6421 cgcgcgcagc tcggcgaagc agtcgccgat cgaggcgtgc gtgcgcaggt cgagccagcg
     6481 gtgagcgctg acggaaatac caccggcgat ctccggaact tcctcgacgt cgtagaccag
     6541 atgggccgtc gcagcgccga ccgcatcgca actgcgcagc atcgcgctga cgttgtgccc
     6601 gtcgtagatg ttctccatca cgaccgccag atccggctga cgacgggcca aggtcccgcg
     6661 catccgtgcc aatcgtcgcg ccgtgatcgg cttcccggct gcctccacgt catctcccca
     6721 ttcagaccaa gcagcgtata ctcaagcgaa tggtgcctgt ccggacagga ttgcggcaac
     6781 ccgtatgtga agggtgaatc ctgatggtcc gtgccatcaa taccgatcac cggctcacgt
     6841 tcgaggaata cctcgccttc gaagagacca gtgacgtacg tcacgagttg gtggacggtc
     6901 acttgtatgc attcgctgga gccagcgatc gacataattt gatttgttcc aacttgatcg
     6961 gcacactctg gatgcacgcc cgtgggtcca gttgtcgcgt gttcggtagc gacatgaagg
     7021 ttcgtgtctc gtctcgagtc ggttactatc ccgatgtgat ggtggcgtgc gacccggagg
     7081 acaaccactc actctaccgg agccgcccgt gcctgctggt cgaggtcctc tcaccaagca
     7141 ccagactcgt cgaccgccga gaaaagctga tcgcgtatca gtccatcgca tcgttgcggg
     7201 gatatctgat ggtctatcgc gacgaatacc gcgtcgaggc gtatacacga aatggcgacg
     7261 acgtctgggt ctatgcgaat ctcaccactg acgacaatgt ctggtttccg tgcattgacg
     7321 tgacagttcc cgtcatggcg ctctacgagg atgtcgacat gtcgccgatc gtcccgacgg
     7381 ccgaagaacc cgacattcgc tgattcagcg acctcgacgt gctttgacag tgtctatcag
     7441 agttggatat agttgcggat ggccttcgct cggcgcaggg tgctgtcgcg tgggccgtgt
     7501 tttattgttg gaaacgcact cgatccctgg gagcggcgga agaggagagc ctcgtgccga
     7561 agcgcacata ccagccaaag cgactgcatc gcaagaagga tcatggtttc atgaagcgca
     7621 tgtccacccg cgccgggcgc gccgtgctgg ccgcgcggcg gcgcaagggg cgccattcgc
     7681 tcagcgtgag caccgagtac aagccgacgc aaaagaagta gcgccggccg tgagtcgccc
     7741 gaggacccac cctggccagc cgggtgggtc ctggcatctg gccggagttg ctgccgatgg
     7801 ttgcccgtga gctacgcctc cggcgtggaa gcgatatcga ccgcgtacgc tcaaagggcc
     7861 ggagctggtc gtcgcgattg ctcgtgctgg caatcctgcc gaacgggttg gagcacaacc
     7921 gctacggctt cacggtcggc aagcgggtgg gaggatcagt cgaacgcaac cgtgccaagc
     7981 ggctgatgcg tgaggcgatc cggcactgtc atccgacact ggtgcaggga cacgacgtgc
     8041 tgctgatcgc acgcaatagc ttccgcgatg agacaacgct cgcggaggtg gcggagcaac
     8101 tggatggcct gctgcggcgg gcgggtatcc gatcggcgga aacaggatga gacggctcgc
     8161 acttctgctg atacgcggct accagcgcgg catttcgccg atgctgccac cctcgtgcag
     8221 attcgtgcca acctgctccg attacggcta ccaggcgatc gagaagtacg gtatcatccg
     8281 gggcggcgcg atgacggtct ggcgcatcat gcgctgcaat ccgttcaaca agggtggatt
     8341 cgatcc
//
LOCUS       DASUGJ010000161         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1967335, whole genome shotgun sequence.
ACCESSION   DASUGJ010000161 DASUGJ010000000
VERSION     DASUGJ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967335"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFV93_03485"
     CDS             complement(<1..202)
                     /locus_tag="VFV93_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164436.1"
                     /translation="MVETLITGGHLLTMAGDGVGFVEDGAVAIDGGKIVAVGPRAEVE
                     EQAGNAGQTIDATDRLVMPGLVD"
     gene            complement(409..945)
                     /locus_tag="VFV93_03490"
     CDS             complement(409..945)
                     /locus_tag="VFV93_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164437.1"
                     /translation="MQTDEGKTTMQATAPKTRFEFGRFGGSLAAVGMVAAFAVGIVTG
                     IAGQAIIDETTDATGTASAIVQPKAHYSAGQGEGLLAVGAGVSAVKAYDSEGMGEGRL
                     ANGMSLATLPLAYTMAGQGEGILGGHNTRAVLASPVVAYNAVGMGEGWLAYGRPQTTI
                     RAYPSDGQGEGWVGNGRP"
     gene            complement(1092..2852)
                     /locus_tag="VFV93_03495"
     CDS             complement(1092..2852)
                     /locus_tag="VFV93_03495"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5164438.1"
                     /translation="MKMLTPQMLHDRLEHRLPLLTGGSRDLPERQQTLRDTIAWSYDL
                     LDSVQQALFRRLSVFSGGFTVEAAVALMPEANETDVLDILSTLTDQSLVRRATVRDAS
                     PASTAEVRLVLLETIREYGLERLSADGETERLRARHASFFADLAERAAPELHGPGQLD
                     WLERLDVEHDNLREALRWSLEHSPDIALAMAGALGWYWYIRGSFVEGSQWLQQALRRV
                     GNGHTTVGAQAHCALGMLLRELAEFGEAAEQLDASVRICRELNDGDGLIVALNERAFV
                     GVYQGHVDESLPLFNEGLQLAIARGDRHGEAYYLSALGLLEFHLSGDPQRSLPFSEKS
                     LSLFREVGDLHEIAIELGHVGYAALYRGDYQLAARYLDEGLALARQLGDHLVASFLLV
                     LLGFLALDQNDLILARERFADSIDLCQRLRMRGRMLGCLEGLAGVAAQSGAADRASTL
                     LGAVETWRERLGQPIQPAEQTRHAKIVAETRRRIDPAAYTQSWNEGAELTLEAATALA
                     LESLPEAAAAPIPSPFGLTARELDVLRLVAQGLTDTEVAEQLFIARRTVNTHLTSIYT
                     KLNVNSRAAATRFAVERGLS"
     gene            3165..3503
                     /locus_tag="VFV93_03500"
     CDS             3165..3503
                     /locus_tag="VFV93_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164439.1"
                     /translation="MLEIVQHEQYRPLADDISQADQWRFITLLANTERRGNGRGNPVS
                     VGDRSELNKDNLIRVHVRQSTRNLNCQPGLANSARAGQRHKTDSILAEQIADRIDIRV
                     STDEVRERCR"
     gene            complement(3669..>5075)
                     /locus_tag="VFV93_03505"
     CDS             complement(3669..>5075)
                     /locus_tag="VFV93_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX5164440.1"
                     /translation="LESIAGQVASGSNFLYFVTGNGSITNFPFVPTIKIVTTTGRFAL
                     LEHDMDVNAGAYLDGTPMDELGQDLLARTIAAASGERTAGEQAGHSQVSIWRDWPLTG
                     PGELAAVQALPVPTGEPLLRASDIVLDDNERRLASRTFRAIVSERGPVTDRVGLVLPT
                     SLCSGQIARRIAGWLDQQHAGEGGISRYVALPHTEGCGASSGSSEEIYIRTLIGHLTH
                     PLVAPALLLEHGCEKTHNDYLRHALRQRELDPSRFGWASIQLDGGIDAVERKVSGWFA
                     TACSAAPPLEYANAGLGWLRLGLAAVGEMSPEASRTYAVLARLVVASGGTVVMPESAL
                     PLASPEGWQTAYGLDSVAPSLGYGEVATTHGLHVMETPTSDWGETLTGLGATGVEIVV
                     AHSPGRPLAAHRMIPVIQTTVAGNPAASDFDLLVGDEVDQLLDLVLRVASREYTPQMT
                     ARDNTEFQITRGRLGISM"
BASE COUNT          953 a         1710 c         1535 g          877 t
ORIGIN      
        1 catcgaccag ccccggcatc accagccggt cggtcgcgtc gatcgtctga ccggcgttgc
       61 ccgcctgctc ctcgacctcc gcccgtggcc cgaccgcaac gatcttcccg ccgtcgattg
      121 cgaccgcgcc gtcctcgacg aacccgaccc catcacccgc catcgtcagc aggtgtccac
      181 ccgtaattag cgtttcgacc atgtgcccgc tcctcccagc aattccgtcg gatggtaccg
      241 caaacagaac agcctatcgc ccgcgagacc cggcaccggt cacgtagttg cctgcttcct
      301 cttccacggc ttgagggtgg catgtggggg ttcaacagca cacgcgatga agccccgcca
      361 gtgcggggcc tcagtcgtcg ctcatctgtg gatccggcat tgcaggaact atggtcgtcc
      421 gtttccgacc cagccttcgc cctgaccgtc cgacggatag gcgcgtatcg tcgtctgcgg
      481 acgtccgtaa gccagccagc cttcgcccat cccgacggcg ttgtaggcga cgaccggcga
      541 ggccagcacc gcacgggtgt tgtgtccacc gaggatgcct tcgccctgac cagccatcgt
      601 gtatgccagc ggcagtgttg cgagcgacat gccgttggcc aatcgcccct cgcccatgcc
      661 ctcggagtcg taggccttga cggccgagac acccgcgcca actgccagca gcccctcgcc
      721 ctgaccggcg ctgtagtgcg ccttcggctg gacgatcgcc gacgcagtcc cggtcgcatc
      781 ggttgtctcg tcgatgatcg cctgcccggc gatgcccgtc acgatgccca ccgcaaatgc
      841 cgccaccatg cccaccgccg caagagaacc accgaaccgc ccgaactcga atcgcgtctt
      901 cggagctgtt gcctgcatcg tcgtcttccc ttcatccgtc tgcactcgat ctcgttcgat
      961 ggcatcagat tagggatttc cagttggcag cacatcgttc agacaacgtg tctttggaac
     1021 aggcagcaat ccaacgcgac tacgttatct aacgtatcgc gctatcgaat cagcgcgccg
     1081 gtcgcggccg gtcagctgag gccacgctcg acagcgaacc gtgttgcggc cgcgcgcgag
     1141 ttcacgttga gcttggtgta gatcgaggtc aggtgggtat tgactgtccg acgtgcgatg
     1201 aacagctgct ccgcaacttc agtgtccgtc agaccctgcg cgacgaggcg caacacatca
     1261 agctcacggg cagttagccc aaaaggcgac gggatcggag ccgccgctgc ctcaggcaac
     1321 gactccagcg ccagcgctgt ggcggcttcc agcgtcagtt ccgctccctc gttccaggac
     1381 tgcgtgtagg ccgctggatc gatgcgtctc cgtgtttccg cgacaatctt cgcgtgccgt
     1441 gtttgctcag cgggctggat cggctggccg agtcgctctc gccatgtctc gaccgctcca
     1501 agcagggtcg aagcccggtc ggcagccccc gattgtgccg caacaccggc caatccttcg
     1561 aggcatccca gcatccggcc ccgcatgcgc aatctctggc acaggtcgat tgagtcagcg
     1621 aagcgctcac gggcgagaat cagatcgttc tgatccaatg ccagaaatcc gagcagcaca
     1681 agcaggaacg aggcgacgag gtgatccccc agctgccgcg ccagcgccaa tccctcatcg
     1741 aggtatctcg cggcaagctg gtagtcacct cgatacagtg ccgcatagcc gacatggcca
     1801 agttcaatgg caatctcatg aagatcgccc acctcgcgga acaggctcag gcttttttcg
     1861 gagaacggca gtgagcgttg gggatctccg gagagatgaa attcgagcag accaagggca
     1921 ctcaggtaat acgcttcgcc atgccgatca ccgcgggcga tcgccagttg caaaccttcg
     1981 ttaaagagcg gcagggattc gtcaacgtga ccctgatata ccccgacaaa cgctcgctcg
     2041 ttcaaggcga cgatcaggcc gtcaccatcg ttgagctcgc gacagatgcg aaccgaggca
     2101 tcgagctgtt ctgcggcctc accgaattct gcgagttcac ggagcagcat tccaagcgcg
     2161 caatgggctt gggcacctac cgtggtatgg ccgttgccaa cgcgtctcag tgcctgctgc
     2221 aaccactgag atccttcgac aaagctgcca cggatgtacc agtaccaacc aagcgcaccg
     2281 gccattgcca acgcgatatc gggactgtgc tctagcgacc aacgtaacgc ttcgcgcaga
     2341 ttgtcatgct ccacatcgag gcgttcgagc cagtccaatt ggccggggcc atgcaattcc
     2401 ggcgcggcgc gttctgccag gtctgcgaag aaggatgcat gacgagcgcg taatcgttcg
     2461 gtctcgccgt ctgcgctgag acgttccaac ccgtactcgc ggatcgtttc aagcaggacc
     2521 aaccgtacct cggcggttga cgccggcgat gcgtcgcgca ctgtggctcg tcgaaccagg
     2581 ctctgatccg tcagtgttga gaggatgtcc agaacgtcgg tttcgttcgc ttcgggcatg
     2641 agcgccacgg ccgcctcgac ggtgaacccg ccactgaaga ccgaaagtcg cctgaacaac
     2701 gcctgctgta cggagtcgag caggtcgtaa ctccaggcga tcgtgtcacg cagggtctgc
     2761 tgccgttccg gcaagtcacg cgagccgcca gtcagcagcg gcagacgatg ctcgagtcga
     2821 tcatggagca tctgaggggt cagcatcttc attcgcgctg ccgccaactc gatcgccagt
     2881 ggcaacccat cgagacggcg gcagatgctg gagatgagcg gaccgttctc accagtcaac
     2941 cgaaagtctg gctggatctc ccgtattcgc tcgacaaaga gcgcgatagc accgaatgcg
     3001 tctagcacgt cgccggtcgc tttcaccgca gccgggactt ccagcggcgc taccgcgaac
     3061 tcacgctcac cccggatccg taaccgggcg cgacttgtcg ccagcacccg cagattggaa
     3121 cagccggtca gcagatcggc aacgaacgat gcggcgtctg gtacatgctc gaaattgtcc
     3181 agcacgagca gtatcgaccg ctcgccgatg acatcagtca ggctgaccag tggcgattca
     3241 tcacgctgct cgcgaacacc gaacgccgtg gcaacggccg aggcaacccg gttagcgtcg
     3301 gagatcggag cgagctcaac aaagacaacc tcatccgggt gcacgtccga caatcgacgc
     3361 gcaacctcaa ctgccagcct ggtcttgcca actccgccag ggccggtcaa cgtcacaaga
     3421 cggacagcat cctcgcggag cagatcgcgg atcgcatcga catccgcgtc tcgaccgatg
     3481 aggtgcgtga gaggtgccgg tagcgaccgt gcgcgaccgg gggaacgtgg tgcagtcatc
     3541 ggcaacgccc aacgctcgcg aaggaacgct cacactccca tcatgcgtcc ccaggcaagc
     3601 cccgtcaatc agcggggaac attcgatggt tctcagcaca ccgtcgccgt ttggattatc
     3661 gcgaacgcct acatcgagat gcccagccgc ccgcgcgtga tctggaactc ggtgttgtcg
     3721 cgcgccgtca tctgcggcgt atactcgcgg ctcgcaacgc gtaggacgag atccagcaac
     3781 tgatcgacct catcaccgac cagcagatcg aagtcggagg ctgctgggtt tcctgcaacc
     3841 gttgtctgga tgaccgggat catccggtgg gcagccagcg ggcggcctgg gctgtgggca
     3901 acgacgatct caacgccggt cgcgccgagg ccggtcagcg tctcgcccca gtcgctggtc
     3961 ggggtctcca tcacgtgcag gccgtgggtc gtagcgactt cgccgtagcc gagcgacggt
     4021 gcgacggaat cgagcccgta cgcggtctgc caaccttccg gtgatgccaa cggcaacgcc
     4081 gattccggca tgacgactgt cccgcctgag gcgacgacaa gccgcgccag cacagcatac
     4141 gttcgggaag cctccggcga catctcgccg acagcggcga gtcccaaccg cagccaaccc
     4201 agcccggcat tggcgtattc gagcggtgga gcagccgagc acgccgtcgc gaaccagccg
     4261 gagaccttgc gctcgacagc gtcgatgccg ccgtcgagct ggatgctggc ccagccgaag
     4321 cgggatggat caagttcccg ctggcgcagg gcgtggcgca gatagtcatt gtgggtcttc
     4381 tcgcagccgt gctccagcag cagcgccgga gcgaccagcg gatgggtcag gtggccaatc
     4441 agcgtccgga tgtagatctc ctccgacgag ccggacgacg ccccgcaacc ctcggtgtgc
     4501 ggcaaggcga cgtagcggga gataccgccc tctccggcat gctgctggtc gagccagcct
     4561 gcgatccggc gggcgatctg accagagcag aggctggtcg gcagcaccag cccgacccgg
     4621 tccgtgaccg gaccgcgttc gctcacgatc gcccggaacg ttcgggacgc cagccgccgc
     4681 tcgttgtcgt cgaggacgat gtcgctcgcg cgcagcagcg gctcgccggt tgggacgggc
     4741 agcgcctgaa cagcagccag ctcgccagga ccggtcaacg gccagtcccg ccagatcgag
     4801 acctggctgt ggccggcctg ctcgccggct gtccgttcgc cactggcagc ggcgatcgtc
     4861 cgcgccagca gatcctggcc aagctcgtcc atcggcgtcc cgtcgaggta ggcgccggcg
     4921 ttgacgtcca tgtcgtgttc cagcagcgcg aagcggcccg tcgtcgtcac gatcttgatc
     4981 gtcgggacga acgggaagtt ggtgatcgag ccgttgccgg tcacgaaata caggaaattg
     5041 ctgcccgacg ccacctggcc ggcgatgctc tccag
//
LOCUS       DASUGJ010000162         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1271872, whole genome shotgun sequence.
ACCESSION   DASUGJ010000162 DASUGJ010000000
VERSION     DASUGJ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271872"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="VFV93_03510"
     CDS             <1..1385
                     /locus_tag="VFV93_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5164441.1"
                     /translation="ADVVAAIEAASAAFNRWRLFPAPKRGEILFKVGQLLIERKEQLA
                     REMTEEMGKVIAEARGDVQEGIDMAFYMAGEGRRQWGQTVPAEMPNKWAMSVRKPLGV
                     VAAITPWNFPLAIPTWKIMPALVTGNTVVFKPASYTPRMAVRLVEIFEEAGLPAGVLN
                     LVLGSGPDTGDTLLQHPDVALVSFTGSTETGTHISVEGARQLKRVSLELGGKNAIIVM
                     DDADVDLAVEGILWSAFGTSGQRCTAASRVIAHRGLSDELTGKLAERAGAMRLGYGLE
                     DSTDVGPVVSRSQLERVHSYIAVGQGEGAELAIGGEIVGDGDLGRGHFHQPTVFGNVK
                     PDMRIAQEEIFGPVTAVIPVSDVEEAINVANGVQYGLSASIYTRDVNRAFRAMQDIYT
                     GILYINAGTIGAEIQLPFGGTKATGNGHREAGQAALDVFTEWQSIYVDYSGKLQRAQI
                     DEVEDNGMAP"
     gene            complement(1497..1910)
                     /locus_tag="VFV93_03515"
     CDS             complement(1497..1910)
                     /locus_tag="VFV93_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX5164442.1"
                     /translation="MSATSRSQRELIEDAINKRVWAIVGASENTEKFGNRIYRNLKQA
                     GYEVYGVNPNAKTADGDPVYSTLADLPVKPDVVDVVVPSWVGRRIAEDAARADIPIFW
                     LQPGAESDELIAYATSLGLNVIHDACAMVEKRRWT"
     gene            complement(2005..2097)
                     /locus_tag="VFV93_03520"
     tRNA            complement(2005..2097)
                     /locus_tag="VFV93_03520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2061..2063),aa:Ser,seq:gct)
BASE COUNT          378 a          670 c          736 g          373 t
ORIGIN      
        1 aggcagatgt cgtggcggcg atcgaggcgg cctcggcggc cttcaatcgc tggcggctct
       61 ttcctgcgcc gaagcggggc gagattctct tcaaggtcgg gcagctgctg atcgagcgta
      121 aggagcaact cgcccgcgag atgactgagg agatgggcaa ggtcatcgcc gaagcgcgcg
      181 gcgacgtcca ggaaggcatc gacatggcct tctacatggc cggcgagggt cgccgccagt
      241 ggggccagac ggtgccggcc gagatgccca acaagtgggc gatgtcggtc cgcaagccgc
      301 tcggcgtggt ggccgctatc acgccgtgga acttcccgct ggcgattcca acctggaaga
      361 tcatgccggc gctcgtgacc ggcaacacgg tcgtgttcaa gcccgcgtcg tatacgccac
      421 ggatggcagt ccggctggtt gagatcttcg aggaggccgg gctaccggcc ggcgtcctca
      481 acctcgtgct cggcagtggc ccggacacgg gtgatacgct gctgcagcac ccggatgtcg
      541 cgctggtgtc gttcaccggt tcgactgaga cggggacgca tatcagcgtc gaaggcgccc
      601 gtcagctgaa gcgggtctcg ctggagctgg gtggcaagaa cgcgatcatc gtcatggacg
      661 acgccgatgt cgatctggcg gtcgaaggga tcctctggag cgcgttcggc acgtccggcc
      721 agcgttgcac ggctgccagc cgcgtcatcg ctcaccgggg tttgtcggac gagctgaccg
      781 gcaagctggc cgagcgggct ggggcaatgc ggctcggcta cgggttggag gacagcaccg
      841 acgtcgggcc ggtcgtcagc cgctcgcagc tggagcgggt ccactcctac atcgctgttg
      901 ggcagggcga aggggccgag ctggcgatcg ggggcgagat cgtcggtgac ggcgacctgg
      961 gacgcggcca cttccaccag ccgaccgtct tcggcaacgt caagccggac atgcgaatcg
     1021 cccaggagga gatcttcggg ccggtcacgg ccgtgatccc cgtctcggat gtcgaagaag
     1081 cgatcaacgt cgccaacggc gtgcagtacg gcctgtcggc ctcgatctac actcgcgacg
     1141 tcaaccgcgc cttccgggcg atgcaggata tctacaccgg catcctctac atcaacgccg
     1201 gcacgatcgg cgctgagatc caactcccgt tcggcggcac gaaggctacc ggcaacggcc
     1261 accgcgaggc gggccaggct gcgctggatg tcttcaccga gtggcagtcg atctacgtcg
     1321 actacagcgg caagctgcag cgcgcccaga tcgatgaggt cgaagacaac ggcatggctc
     1381 cgtaaatgtt cgaggcagta ggtgataggt gtcaggggag gcttcgtggg gagggcagtg
     1441 tggtctcgcc cttcacggac cgatccttaa cacctatcac ctactacctc gcgcccctac
     1501 gtccagcgcc gtttttcgac catggcgcag gcgtcgtgga tgacgttgag accgagcgac
     1561 gtggcgtagg cgatcagttc gtcgctctcg gcgccgggct ggagccagaa gatggggatg
     1621 tcggccctgg cggcgtcttc ggcgatgcgg cggccgaccc aggagggcac gacgacatcg
     1681 acgacgtccg gcttgactgg caggtcggcc agtgtcgagt agaccgggtc gccgtcggcc
     1741 gtcttggcat tcgggttgac accgtagacc tcatagccgg cctgcttcaa gttgcggtag
     1801 atgcggttgc cgaacttctc cgtgttctcg gacgcgccaa cgatcgccca gacgcgcttg
     1861 ttaatggcat cttcaatcag ttcacgctgg ctgcggctgg tcgcgctcat ggctcactcc
     1921 tctcatgaat cacaacgcag gggcgttgcc gcgattccgc gaaccgggta tgcaaaagcg
     1981 ccaggcatgt cgcctggcgc ttgctggcgg gaaggcaggg attcgaaccc tgggtagacc
     2041 ttaacggccc acaaccgctt agcaggcggc cccattcgac cactctggca ccgtcccatg
     2101 gggcacgcgt cagatatcgc caccagtcga agccgttgag ccatatcgaa acgcacg
//
LOCUS       DASUGJ010000163        10846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1967452, whole genome shotgun sequence.
ACCESSION   DASUGJ010000163 DASUGJ010000000
VERSION     DASUGJ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10846
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967452"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFV93_03525"
     CDS             <1..185
                     /locus_tag="VFV93_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164443.1"
                     /translation="LVQAGDVAGLKTALCHALADANWRRDVSEAARSFVVAKYDMQVV
                     AARLASVYHEVVGVVG"
     gene            complement(338..1723)
                     /locus_tag="VFV93_03530"
     CDS             complement(338..1723)
                     /locus_tag="VFV93_03530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5164444.1"
                     /translation="MAVAARQSRPQSQNEGDPADWPFVLPEDPLPWDTNAQLRSAFDD
                     AAIGMSIANLNWGLLRVNSAHCRMLGYTEHELLTTDFRTRIHPDDLAPNDDLRRDLFS
                     GVINSYETERRYFHRDGHVVWCLVTVSLIRDADGTPQYFVGQMQDITERKRAEESLQQ
                     SEQRMARVLTSASDGMLIVDQAGIVVMANPAAERITGLLSATLTGAAVRDLPRLVHGH
                     DNPDCAAVTKLFDRALVDGEATQGFEMAFTRADGVTIHGVIDIVPLRGTGGEHDGLLL
                     TVHDVTERKLLEQQLAQLALHDALTGLPNRRLFEDRAEQALAAARRGGWLVGLLLIDL
                     DNFKPVNDRFGHPAGDQVLAEVARRLSGCLRSEDTAARFGGDEFVVLLRSVRGLGGIR
                     EVRERLSRELERPHLLDGEAVTVTASIGTSLTTTADDRLSDLLHHADSSMYAIKREHR
                     RAPDRTSYQLV"
     gene            1987..3828
                     /locus_tag="VFV93_03535"
     CDS             1987..3828
                     /locus_tag="VFV93_03535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5164445.1"
                     /translation="MTVTDSSKPRQRYTFEQFTAVRRYQPALSFSPDGSEVAYSVNIS
                     GQYNLWRQSSAGGYPHQITLYSEQAVRDVQWSPDGEWILYTADHHGDEFYQVYRIPAR
                     GGQPEQLTDAEQVQHYLGQDAWSPDGKAIAYSANDRTPAEQDVLVRDMQTGQVRRVLE
                     GDALYFAAGWSPDGKRLTVVDAKSNTNQDIYLLDPESGKIDHLTPRDGEVKNFPGPWA
                     GDGSGFYVLSDEDREFLGMAFQDVRDGQKRWVETPDWDIEQLAASPDGRYLIWVVNEG
                     GYSRLYARDLSTNQPVTLPEVPGGVIRHLTTSRDGRKIGLLLDRPDHPTEIYVVDIDQ
                     QTTTQITFSFLGGVDPADLIVPEAISYPTHDGREIPAFLYKPQGDGPFAVVLSIHGGP
                     EAQERPLYMYTGLYQYWLSRGIGVLAPNVRGSTGYGSTYQKLIHRDWGGAELKDFEAG
                     VQYLHGLDWVDNQRIGVFGGSFGGFATLSCVSRLPQYFAAGVDIVGPSNLVTFAKAVP
                     PTWRRMMTQWVGDPETEADFLMERSPITYVDQIQAPLFVIQGAQDPRVVKPESDQIVE
                     RLRARGVEVRYDVYEDEGHGFTKRENELRAMHDTALFMETYLSGNEG"
     gene            3906..4331
                     /locus_tag="VFV93_03540"
     CDS             3906..4331
                     /locus_tag="VFV93_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164446.1"
                     /translation="MGRTVSIQFTDEQMTRLEREANRLGQSPVDAAARIIEESLRESE
                     FPRIEFRDSAAGRQAYLRGTRLTAWMIAYIDRSYDGDIEQTSEHLEIPAPEVQSALAY
                     AAAFPDEIAAAISDLDRTPEQLRELIPNLEVFSVNAPAR"
     gene            4405..4677
                     /locus_tag="VFV93_03545"
     CDS             4405..4677
                     /locus_tag="VFV93_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164447.1"
                     /translation="MDGEYRTKPDETLLEIGFETGRVLVSYDVHTIPPLVQEWADAGR
                     QHAGVIFITGRAAQINDVGAILRSLRTLLAEQGDEDWRNRVIYLNP"
     gene            complement(4728..7373)
                     /locus_tag="VFV93_03550"
     CDS             complement(4728..7373)
                     /locus_tag="VFV93_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164448.1"
                     /translation="MNNWKHTKTHLILTILLLSAAIVPFVTPPTSRAAPIANDPFQHT
                     WERTDQPVADGAVSRTWMWGPAPFSDAKLEDYAESPNGQRTVQYFDKSRMELTHPDGD
                     QASVWYVTNGLLVVELMSGRIQVGDNAFIDRAPAQINVAGDPDDPLTYAVLAGVRDAA
                     ALAPGTAVTQRIDAAGVVTDDPSLADYGVTGATHVDETDHTVASPFWAFMTSSGLVDQ
                     DGETTTAPLFDSAYYATGFPITEAYWASVTLGGQPHEVLLQCFERRCLTYTPGNPEGW
                     QVEAGNVGQHYYRWRYPEAPAGPNAQANELAGKVAQAGSDDERYNALLDVMDALHVGV
                     YTGQGEMVLGGAERGAADFYLYDIELRMLAGALGRGQTYGVIDLATQLTEMGILPEGE
                     TLDPDLLRQAILNAAGSAQQTPDDFSGLAILLARQLGLYQLQPYDLFEDVALDDIRLD
                     ALSYFLLLADTSLPFIQDQLPAEGLHSDLIAQTNGVLKPAKASSPCDPSELFKGDAFK
                     DSYGWTKTFAELLKLIPEAVSEVTGFLDAIHGAILAFSINVTELDQRLETHYGPAGHA
                     SEAGQPITFRIKVEMLDKLPQALIDCGWIAGTDFPDVGPIKGVSVLWDWEGLNQYGTL
                     DCGPTCPTAGSNGLAIDATGDDGIAKLVFQPKDEPNPGKGMTIEETGVVTGVALYQSK
                     FTNLLGSYAQFATPKSGATRWFVSHHEEPALKITMTLDYDIVQRLFDDTYVGTGTGTG
                     TAVITIEHPDQAMTDPQTLAPTHYAMSGTYTMEAPIPCQGTWTYDGDGIAVVSPGESP
                     GTVILHLVVDEYDDGKGGWSTDCELGGDTGNLVRLVNYEVALDVGTLQTIPITAAMIA
                     EPWGGADQGVTGPATATFLVEPIVP"
     gene            complement(7739..8653)
                     /gene="merB"
                     /locus_tag="VFV93_03555"
                     /pseudo
     CDS             complement(7739..8653)
                     /gene="merB"
                     /locus_tag="VFV93_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
     gene            complement(8699..9184)
                     /locus_tag="VFV93_03560"
     CDS             complement(8699..9184)
                     /locus_tag="VFV93_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5164449.1"
                     /translation="MATTSRTRELAGEFEAATGELIEVITACNDAQWNLSAANDERSV
                     GVLAHHVAETNGAFAHIVRTLANGETFSPSISMEEVHDLNAQQARDNAKVGKQETLDR
                     LRANSDAILAALGTIDDDFLDRPAGVFGDNELTVNQVIEWIVIGHTAEHLQTIRAAIG
                     G"
     gene            9555..10457
                     /locus_tag="VFV93_03565"
     CDS             9555..10457
                     /locus_tag="VFV93_03565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX5164450.1"
                     /translation="MTDQTAGSAGMTLVERPEPVPAINDVVVQVHASGYVPTELTWPS
                     TWTDRAGRERTPTIPGHELAGVVTALGYGTTGLSVGQRVFGLADWYRDGTLTESIAIE
                     ARNLAPLPGDVDFTVGASLPISGLTAWQGLFLHGRLQAGQRVLAHGAAGAVGSMVTQL
                     AREAGAYVIGTGRAADRQTALDFGALEFVDLEHDALEDAGGVDLVFDVIGGDIGQRSA
                     GLIRAGGTLVSIVGPSETRPADGRAVDFVVVADRAQLGEIVQRVRDGRLRTNIGTVAT
                     LDDAVAAFNPTERIKGKTIIRVRP"
     gene            complement(10485..>10846)
                     /locus_tag="VFV93_03570"
     CDS             complement(10485..>10846)
                     /locus_tag="VFV93_03570"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEX5164451.1"
                     /translation="QDIGPIDALKESGRLFKRTWGEQVVGNAGIGLFTFIIGLPLVIV
                     AIVIAGIGFATGSIPVIVLTLIIAGLLIGLTVAVGSTLSGIYRTVLYRWAITGETTPG
                     FPQEAIRASFVPKGAHI"
BASE COUNT         1883 a         3483 c         3559 g         1921 t
ORIGIN      
        1 tgctcgtgca agccggcgac gttgccggcc tcaaaactgc cctctgtcac gcactggccg
       61 acgcgaactg gaggcgtgac gtatccgaag cggcacggtc gttcgtcgtc gcaaagtacg
      121 acatgcaggt cgtcgcggcg cggctcgcca gtgtctatca tgaggtcgtt ggagttgtgg
      181 ggtaatccgg cgtcaatcca atacccaatg gtcaatccgg tacgggcgat atcgcaatat
      241 cgccctttcg gacgcccggc gcttgggaag ggcgatattg cgatatcgcc cgtaccggag
      301 tggggattgc attggatgaa tggctggcgg ggaacgatca gaccagttgg tagctggttc
      361 gatccggcgc tcgccggtgt tcgcgcttga tggcgtacat gctgctgtcg gcgtgatgca
      421 gcagatcgct gagccggtcg tcggccgtgg tcgtcaggct ggtcccgatg ctggcggtca
      481 ccgtcactgc ctcaccatcg agaagatgcg gccgctccag ctcccgcgag agacgttcgc
      541 ggacctcgcg tatgccgccg agaccacgta ccgaacggag caggacgacg aactcgtcgc
      601 cgccgaagcg ggcggcggtg tcctcagatc gcaggcagcc tgagaggcgc cgcgcaacct
      661 cggccagcac ctgatcgccg gccggatggc cgaagcggtc gttgacgggc ttgaagttgt
      721 ccaggtcgat cagcagcagg ccgaccagcc agccgccgcg ccgcgccgcc gccagcgcct
      781 gctcggcgcg atcctcgaac agccgccggt tcggcagccc cgtcagcgcg tcgtggagcg
      841 ccagttgggc aagctgctgc tccagcagct tgcgttcggt gacatcatga acagtcagca
      901 acaggccatc gtgctcgcca cctgtgccgc gcagcggaac gatgtcgatg acgccgtgaa
      961 tggtcacccc atcagcccgc gtaaacgcca tctcgaagcc ctgcgtcgcc tcgccatcga
     1021 ccaacgctcg gtcgaagagc ttggtaactg cggcgcaatc cggattgtca tgcccatgca
     1081 ccagccgggg tagatcgcga accgccgccc cggtcagcgt tgctgagagc aatccggtga
     1141 tgcgctcggc agccggattg gccatcacga cgatgccggc ctgatcgacg atcagcatcc
     1201 cgtccgacgc ggaggtcagg acacgtgcca tccgctgctc gctctgctgc agcgattcct
     1261 cggcgcgctt gcgctcggtg atgtcctgca tctggccgac gaagtactgc ggcgtgccgt
     1321 cagcatcgcg gatcagcgag accgtcacca gacaccagac aacgtgacca tcgcggtgga
     1381 agtagcgccg ctcggtctcg taggagttga tcacgccgga aaacaggtcg cgacgcaggt
     1441 catcgttggg cgccaggtcg tcggggtgga tccgggttcg gaagtcggtc gtcagcaact
     1501 cgtgctcggt gtagccgagc atccggcaat gggccgagtt gacgcgcagc aacccccagt
     1561 tgagattggc aatcgacatc ccgatcgcgg cgtcgtcaaa cgcgctgcga agctgggcgt
     1621 tggtatccca cggcaatgga tcctccggca gcacgaacgg ccaatcggcc gggtcgcctt
     1681 cattctgaga ctgtggacgt gactgtcgcg cagcgaccgc catggatcgg gctcctgcct
     1741 cggtccgaat cgtgttgacg catcgaccat ctgaccagtt gaccaattat gtacgcaaca
     1801 gaatgccaca gcgccagcgc caggaataga tgcccgaccg acattgggac gaaagtcacc
     1861 acctgccagg ccaggacacc ggcagcactg cggccgggaa cattgcacgc cggaccgcga
     1921 tgcagaacaa tgaccgacaa cgaaccatga cgtgctattt ggtagcaatt cagggagatt
     1981 caggcaatga cagtcaccga ttcgagcaag ccgaggcagc gctacacctt cgagcagttc
     2041 acagccgtcc ggcgctacca gccggcgctc tcgttctcgc cggacggctc ggaagtagcc
     2101 tactcggtca acatctccgg ccagtacaac ctctggcgac agtccagtgc gggcggctat
     2161 ccgcaccaga tcacgcttta cagcgagcag gccgttcgcg atgtgcagtg gtcaccggat
     2221 ggggaatgga tcctctacac ggccgaccac cacggcgacg agttctatca ggtctaccga
     2281 atcccggccc gtggcgggca gccggagcaa ctgaccgacg ccgagcaggt ccaacactac
     2341 ctcggccagg atgcctggtc gccggacggc aaggcgatcg cctacagcgc caacgaccgc
     2401 acgccggccg agcaggatgt cctcgtccgt gacatgcaga ccggccaggt tcgccgcgtc
     2461 ctggagggcg atgcgctcta cttcgcggcc ggctggtcgc cggacggcaa gcggctgacc
     2521 gtcgtcgacg ccaagtcgaa caccaaccag gacatctacc tgctcgaccc ggagtcgggc
     2581 aagatcgacc acctgacacc gcgcgacggc gaggtcaaga actttcccgg cccatgggcc
     2641 ggcgacggct ccggcttcta cgtcctttct gacgaggacc gcgagttcct cgggatggcc
     2701 ttccaggatg tccgcgacgg ccagaagcgc tgggtcgaga cgcccgactg ggacatcgag
     2761 cagctggcgg cctcgccgga cggacgctac ctgatctggg tcgtcaacga gggcggctat
     2821 tcacgccttt acgcccgtga cctgagcacg aaccagccgg tcacgctgcc ggaggtgccc
     2881 ggcggggtga ttcgccacct gacaacctcg cgcgacggcc gcaagatcgg cctgctgctc
     2941 gaccggcccg accacccgac cgagatctac gtcgtcgata tcgaccagca gacgacgacc
     3001 cagatcacgt tctcgttcct cggtggcgtt gacccggccg acctgatcgt gccggaggcg
     3061 atcagctacc cgacccacga cgggcgcgag attcccgcct tcctctataa gccgcagggc
     3121 gacgggccat tcgcggtcgt cctctcgatc cacggcggac ccgaggcgca ggagcggcca
     3181 ctgtatatgt acaccggcct gtatcagtac tggctctcgc gggggatcgg cgtcctcgcg
     3241 ccgaacgtgc gcggttcgac cggctacggc tcgacctacc agaaactgat ccaccgcgat
     3301 tggggcggcg ccgagctgaa ggacttcgag gcgggagtcc agtacctgca tgggctggac
     3361 tgggtcgaca accagcgaat cggcgtgttc ggcggctcct tcggcggttt cgcgaccctc
     3421 tcgtgtgtct cgcggctgcc gcagtacttc gcagccggcg tcgacatcgt cgggccgagc
     3481 aacctcgtga ccttcgcgaa ggccgtcccg ccgacctggc ggcggatgat gacgcagtgg
     3541 gtcggcgatc cggagacgga ggccgacttc ctgatggagc gctcgcccat cacctacgtc
     3601 gaccagattc aagcgccgct gttcgtcatc cagggcgccc aggacccgcg cgtcgtcaag
     3661 cccgagagcg accagatcgt cgagcgcctc cgcgcgcgcg gcgtcgaggt ccgctacgac
     3721 gtctacgaag acgagggcca cggcttcacc aaacgcgaga acgaactacg cgccatgcac
     3781 gacacggcgc tgttcatgga gacgtatttg tcgggaaatg agggatgaag catgcgggat
     3841 gggggatgag attctcatac ctttccctcg tcccgtacac tgatggcgac gaaggagaag
     3901 tcgcgatggg caggacagtc agcatccaat tcacggatga gcagatgact cggcttgagc
     3961 gcgaggcgaa tcggctcggc cagtcgcctg ttgacgccgc cgcacgcatc atcgaagaat
     4021 cacttcgcga gagtgagttc ccgcgtatcg agttccgaga ttctgctgcg ggacggcagg
     4081 catatctgcg agggacgcgg ctaacggcct ggatgatcgc gtacatcgac cgttcctatg
     4141 acggtgacat tgagcaaacg tcggaacatc tcgaaatacc ggcacctgaa gtccagtcag
     4201 cactcgccta tgcagcagcg ttcccagacg agattgcggc ggcgatctcg gatcttgacc
     4261 gcacgcccga gcaacttcgc gagttgatcc cgaacttgga agtgttctcg gtcaatgcgc
     4321 ctgctcgttg aaacacatat acacgccgct gtcacgaagc ggttagctga tgacggcgtc
     4381 gatgcagtgt cataggcaag gtggatggac ggagagtatc gcaccaagcc cgatgagacc
     4441 ctgcttgaaa tcgggttcga gaccggcagg gtgctcgtct cctacgacgt tcacacgatt
     4501 cctccgctgg tacaggaatg ggctgacgcg ggccgccaac atgcgggggt tatctttatc
     4561 accggtcgag cagctcagat caacgatgtt ggagcgatcc ttcgcagtct ccgcaccctg
     4621 ctcgcggaac agggtgacga agattggcgg aatcgcgtca tctacttgaa tccgtagcac
     4681 ggagtcggat cgcggggata ggagatcccc acgatccgac tccagcccta tggaacgatc
     4741 ggctcgacca ggaacgtcgc ggtggctggc ccggtgaccc cctgatcggc tccgccccaa
     4801 ggctcggcga tcatcgcggc tgtgatcggg atcgtttgca acgtgcctac atcgagtgcg
     4861 acctcatagt tcaccaaccg aacgagattt ccagtgtcgc ccccgagctc acagtcggtt
     4921 gaccatccgc ccttaccatc gtcatactcg tcgacgacga gatggaggat gaccgttccg
     4981 ggcgactcac ctggacttac gacggcgatg ccgtcaccgt cgtaggtcca ggtgccctga
     5041 cagggtatgg gcgcctccat cgtgtaggtg ccggacatcg cgtagtgggt tggagccagg
     5101 gtctgtggat ccgtcatcgc ctgatctggg tgctcgatcg tgatgacggc cgtacccgtg
     5161 ccggtccccg tcccgacata cgtatcgtca aacaagcgct ggacgatatc gtagtcgagt
     5221 gtcatcgtga tcttcagggc cggctcctcg tggtgactga cgaaccagcg cgttgcgccc
     5281 gacttgggcg tcgcgaattg cgcgtaggag ccaagcaggt tggtgaactt ggactggtag
     5341 agcgccacgc cggtcacaac gccggtctcc tcaatcgtca tgcccttgcc ggggttcggc
     5401 tcgtcctttg gctggaagac cagcttcgcg atcccgtcgt cgccggtcgc gtcgatggcg
     5461 aggccgttcg agccggcagt cgggcaggtc gggccgcagt cgagcgtgcc gtactggttc
     5521 agcccctccc aatcccagag gaccgagaca cccttgatcg gcccgacatc ggggaagtcg
     5581 gtcccggcga tccagccgca gtcgatcagt gcctgtggca gcttgtcgag catctcaacc
     5641 ttgatgcgga acgtgatcgg ctgaccggcc tcgctggcgt gaccagccgg tccgtagtgc
     5701 gtctcaaggc gttggtccag ctcggtgaca ttgatcgaga aggccaggat cgcgccgtgg
     5761 atcgcatcca ggaacccggt cacctcgctg accgcctcgg ggatgagctt gagcaactcg
     5821 gcgaacgtct tcgtccagcc gtaggagtcc ttgaaggcgt cgcccttgaa cagctccgac
     5881 gggtcgcacg ggctgctggc tttggccggc ttgaggacgc cgttcgtctg cgcaatcaga
     5941 tcggagtgca gcccctcggc cggcagctga tcctggatga agggcagcga cgtgtcagcc
     6001 agcagcagga agtaggacag cgcatcgagg cgaatgtcgt ccagcgcgac atcctcgaac
     6061 aggtcgtacg gctgcaactg atacagcccc agttggcgcg ccagcaggat cgccagcccg
     6121 ctgaagtcgt ccggtgtctg ctgggcggaa ccggccgcgt tcaggatcgc ctgccgcaac
     6181 aggtcgggat cgagcgtctc gccctccggc aggatcccca tctcggtcag ttgcgtggcg
     6241 aggtcgataa ccccgtaggt ctgcccgcgc ccgagtgcgc cggccagcat tcgcaactcg
     6301 atatcgtaca ggtagaagtc ggccgcgcct cgctctgccc cgccgaggac catctcgccc
     6361 tgaccggtgt agaccccgac atgcagcgcg tccatcacgt ccagcaacgc gttgtagcgc
     6421 tcgtcgtcgc taccggcctg ggccaccttg ccggccaact cgttcgcctg cgcgttcgga
     6481 ccagccggcg cctcgggata gcgccagcgg tagtagtgct gcccgacgtt gccggcctcg
     6541 acctgccagc cctccgggtt gccgggtgtg taggtcagac aacgccgctc gaagcactgc
     6601 agcagcacct catgcggctg cccacccagc gtgacactgg cccagtaggc ttcagtgatc
     6661 gggaagccgg tcgcgtagta ggcgctgtca aacagcggcg ccgtcgtcgt ttccccatcc
     6721 tggtcgacca gcccactgct ggtcatgaag gcccagaacg ggctggcgac ggtatggtcc
     6781 gtctcgtcga catgtgtggc gccggtcacc ccatagtccg ccagcgacgg gtcgtcggtg
     6841 accacgcccg cggcatcgat gcgctgggtg acggccgtcc ccggcgccag cgccgccgcg
     6901 tcacggacgc cggccaggac cgcgtaggtc agtggatcgt ccggatcgcc ggccacgttg
     6961 atctgggccg gggcacggtc gatgaacgca ttgtcgccga cctgtatccg gccactcatc
     7021 aactcgacga ccagcagccc gttggtgaca taccagaccg aggcctggtc gccgtcagga
     7081 tgggtgagct ccatccggga cttgtcgaag tactgcaccg tgcgctggcc gttgggcgac
     7141 tcggcgtagt cctcgagctt ggcatcgctg aagggtgccg gtccccacat ccaggtgcgt
     7201 gagacggcgc cgtcggcgac cggctggtcg gttcgttccc aggtgtgctg gaacgggtcg
     7261 ttcgcgatcg gcgcggcccg gctggtcggt ggcgtcacga acggaacaat cgcggcgctg
     7321 agcagcagga tcgtcaggat cagatgcgtc ttcgtgtgtt tccagttgtt cattgaactc
     7381 ttgtcctcaa ggcatcacga gacatgcgcg atcgcgccgg aggtgtgctc cgacgtagcg
     7441 ctcctgaagc gtctgtggcc cattgatgcg cgtctcttca ccggcgcccc ccgcttcgcc
     7501 ggtgagctac agggccgatc ctggcacaca caaaacacca cgccaaaaga tcgccgtggt
     7561 gagtcgccag cagcgaatgc gtggaggatg tctgtgtgcg ctgttagcag atgggggtca
     7621 gtatatatcg ggatacgatg tatcgcaagc gagcggcata cggaaaccaa ggaccagaga
     7681 acaggcgctc attgcggatg ggcgatcccg gttccctgat tctcgcgcgg tgccgttcct
     7741 acacctgcca gaacgccgag gtcagcccgg cccgggcaaa gacggcgcct gcctgctcgg
     7801 cgctgcggcc ccggaagtcg gggtcgattc gattggcata ccagagtttc gagagttccc
     7861 agatctgcga cagcggcacg atctcgccgc gaggttcgcc ggtcgtcgca caccagccgt
     7921 tgatgtcctc ttccgaccgg aagagcatca tcgtcgctca ggtgtggatc tggtcgtcgt
     7981 accagcgggc gaacgggagc gggaagtgga ccagcccatc gtcgccacgc aacttgccac
     8041 cctcgatcgc gtattgggcg ggtgagcgcg agccgtcggg gttggtgtag acggcctcga
     8101 tcgtcgcgtc ggcatgcagc atggccggga cgccgagcga gtcccaggcg cagttggcga
     8161 agtacgttcg accgttgacg gtcacccggt acggcgtctc gacggccgag agcgggttcg
     8221 ccatccgcac ctgggtcgtg ccgggatcga gcaggagggt atgcgcgtcg tgcagccgct
     8281 ggtacacctc gcgcacctcg tcgtcagcca ttccgaacgc cgcagcggtc tcgctgacgc
     8341 tcgggggccg cgtgtgctcg acgaagtacc ggtagatgtg caggcgcacc tgccagtcac
     8401 gttcgctcag ggatgccgcc atgtctgcct cccgtgtcgt ctcatgctgc gtctggccgt
     8461 gcagtctacc gcaggcgggg aggggtgtgg aatctgaccc aggcggacgg cgctggcgag
     8521 gaaccgaccg tgccaccctt gtcatcccga cgcaggaggg accttgttgc tcgttaacgc
     8581 gggcacgaac cgaacgagaa cgagatccct cgctagcgct caggatgaca gggcaagggg
     8641 gtcggcttct catcagcgct gctcgcctgg atcgtctctt tcggctggct tccccgcatc
     8701 aaccgccgat cgccgcgcgg atggtctgca ggtgctcggc ggtgtggccg atcacgatcc
     8761 attcgatgac ctgattgacc gtcagctcgt tgtcgccgaa gacgccggct ggtcggtcga
     8821 ggaagtcgtc gtcgatggtc cccagcgccg ccaggatcgc gtcgctgttc gcgcgcagcc
     8881 ggtcgagcgt ctcctgcttc ccgacctttg cgttatcgcg tgcctgttgt gcgttgaggt
     8941 cgtggacctc ctccatcgag atcgatggcg agaatgtctc cccgttcgcc agggtccgga
     9001 cgatatgggc aaacgctccg ttcgtctcag cgacgtgatg tgccagcacg ccgaccgatc
     9061 gctcgtcgtt cgcagccgac aggttccact gtgcatcgtt gcatgccgtg atgacctcga
     9121 tgagttcgcc ggtcgccgcc tcgaattctc cggcgagttc acgcgtccga gaggtcgtcg
     9181 ccatcctgaa acctcctgat tgatcctggc tgggcaaccg ggccgctgcc gcccgttgcc
     9241 cggctcgctc ctgacatcgg ccaaccgccg atgcatcgta gtcgattggg accgattcga
     9301 atcgacagtt ggcgggagtc gggaacgaga acctggtatc gaggtaggcc ggcaggcgat
     9361 gttccggcgg tacgtggatg cagaatgcca ccgaattggt ccacggcttc cgcgggtggt
     9421 cgcagtattg ctgtggttcg gaagtggtgt gatgagccgc actggctcgt tcgattccct
     9481 ctcgtgcagg tgaatcgacc atgccgtgct ggcgagagtt agagacagag aggtgccgga
     9541 tgaaggcgat tgtggtgacg gatcagaccg cggggtcggc cgggatgacg ctcgtggagc
     9601 ggcccgagcc ggtcccggcg atcaacgacg tcgttgtgca ggtgcacgcg tccggctacg
     9661 tcccgaccga actgacgtgg ccctcgacct ggaccgatcg cgccgggcgt gaacggacgc
     9721 cgacgattcc cggccacgag ctggccggcg tggtcaccgc cctcggctac ggcacgaccg
     9781 ggctgtcggt gggtcagcgg gtgttcggtc tcgccgactg gtatcgcgac ggcaccctga
     9841 ccgagtccat cgccatcgag gcgcgcaatc tcgcgccgct gccgggcgat gtcgatttca
     9901 cggtcggcgc gagcctgccc atctcgggcc tgaccgcctg gcaggggttg ttcctgcatg
     9961 gccgcctgca ggcgggacag cgcgtcctcg cccacggcgc ggccggcgcc gtgggatcga
    10021 tggtgacgca actcgcgcgc gaggccggcg cctatgtcat cggcactggc cgcgctgccg
    10081 accggcagac ggcgctcgac ttcggcgcgt tggagttcgt cgacctcgag cacgatgcgt
    10141 tggaagacgc cggcggcgtc gatctggtgt tcgacgtcat cggtggcgac atcgggcagc
    10201 ggtcggctgg cctgattcga gccggaggaa cgctggtgtc catcgttggg ccgtccgaga
    10261 ctcgacccgc cgatggacgc gcggtcgact tcgtggtcgt ggccgatcgc gcgcaactgg
    10321 gtgagatcgt ccagcgcgtg cgtgacggac ggctgcggac gaatatcggc accgtcgcga
    10381 ccctcgacga tgctgtcgcc gccttcaacc cgaccgagcg gatcaagggg aagacgatca
    10441 tccgcgttcg cccgtaacgc cttggggcca gcgactgcac tctcttagat gtgtgcgccc
    10501 ttcgggacga acgaggcgcg gatggcctcc tgcgggaagc cgggcgtcgt ctcgccggtg
    10561 atcgcccagc ggtagaggac ggttcggtag ataccgctca gggtcgagcc gaccgcgacc
    10621 gtcaggccga tcaggagtcc cgcgatgatc agcgtcagga cgatgaccgg gattgagccg
    10681 gtcgcgaagc cgatgccggc gatgacgatg gcgacgatca ccagcggcag gccgatgatg
    10741 aacgtgaaca ggccgatgcc ggcgttgccg accacctgct cgccccaggt gcgcttgaac
    10801 agccgcccgc tctccttgag ggcgtcgatc gggccgatgt cctgcg
//
LOCUS       DASUGJ010000164         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_160771, whole genome shotgun sequence.
ACCESSION   DASUGJ010000164 DASUGJ010000000
VERSION     DASUGJ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160771"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..598
                     /locus_tag="VFV93_03575"
     CDS             302..598
                     /locus_tag="VFV93_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164452.1"
                     /translation="MNISARSGWAPEVARRGCTLPPGAAWNGFVLPDVPTAEWPASPL
                     FEQGLDRLVRDALATGRSIHDILTSVAALAQSLAMPMSETDLTRRVKAHVIANT"
     gene            complement(658..990)
                     /locus_tag="VFV93_03580"
     CDS             complement(658..990)
                     /locus_tag="VFV93_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5164453.1"
                     /translation="MGTRIRELRIERGLSLSAFAERTGISPGHLSRIERGITAPSFIA
                     GAALANALGVTADALAVESRSQRARNKELVATLVNAGMDPEIADEIQSVISTTAREQL
                     VHVLVGRR"
     gene            complement(1104..1508)
                     /locus_tag="VFV93_03585"
     CDS             complement(1104..1508)
                     /locus_tag="VFV93_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5164454.1"
                     /translation="MAHILIVEDDPVINDLLAFIMEQDNYILTRALDGKAALGLALQE
                     RPDLILMDLRLPTVDGGDAIRILKSNPETREIPIIAVSASSTLRAEAAGLPVDAVISK
                     PFDVDSLLAEVVVQLQRSAAYEATPIPARLDT"
     gene            complement(1769..2185)
                     /locus_tag="VFV93_03590"
     CDS             complement(1769..2185)
                     /locus_tag="VFV93_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX5164455.1"
                     /translation="MREVFSRFAAQTAAAMGHYWAFIISTLACIVWAVSGPFFGFSDT
                     WQLVINTGTTVLTFLMVFLIQNTQNRDARAVQLKLDELLRAVQKASNSLIDIEEASDD
                     VLERLKDKYRQQALQAHPDDGHERIEAAGGKENAAV"
     gene            2915..>3307
                     /locus_tag="VFV93_03595"
     CDS             2915..>3307
                     /locus_tag="VFV93_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5164456.1"
                     /translation="MALRDQWYKNAIVYCLDVETYADADGDGIGDFRGLTQRLDYLSG
                     LGVTCLWLMPFYPTPNRDDGYDVADYTTVDPRFGTMADFTDFMVEAHDRGLRVILDLV
                     PNHTSDQHPWFQEARRDANSPYRNYYVWR"
BASE COUNT          639 a         1043 c          978 g          647 t
ORIGIN      
        1 cgtcgcagac ccgctccccg gatctgctcg attggtccta ccacaggcaa tcgcaccacc
       61 gacaatcagg acgatacaga caacgagcaa ccaatatccg cgcctcatgg gaaacccaag
      121 cgctacctcc tgacagcatg acccatccgc cgtgcacacc ggcgcagaga gcgtgccgcc
      181 gggcagttcc tgctggttct agcacaaatg ggccagatcg acggcgcggc attcaggacc
      241 cgagcgttgg cgggtgacgc tgacacaggg tacggtagta aatatgaaac gtatgatgtc
      301 cgtgaacatc tccgcacgct ccggctgggc acccgaggtc gcccggcgcg gttgtaccct
      361 gccaccgggt gccgcctgga acgggtttgt gcttccggac gtgccaaccg ccgagtggcc
      421 ggcctcgccg ctgttcgagc agggtctgga ccggttggtt cgtgatgccc tcgcgacggg
      481 ccggtcgatt catgacatcc tgaccagcgt cgccgcgctt gcccagtcgc tcgccatgcc
      541 catgtcggag accgacctca cgcgccgtgt caaggcacac gttatcgcca atacataggc
      601 aaccctgttc gggaacatga ggtcgatccg gttgctcctg cggcgagatc gagtgcgcta
      661 tctgcggccg accagtacat gcaccaactg ctcgcgcgcc gtcgttgaga tcacgctttg
      721 aatctcatcg gcaatctcag ggtccattcc ggcattgacc agcgtggcga cgagttcctt
      781 gttccgcgca cgctgcgatc ggctctcgac ggccagagca tcagcggtaa caccgagcgc
      841 gttggcaagc gcagcgcccg cgatgaacga tggggcggtt atgccacgct cgatccggga
      901 caggtggccg ggggagatgc cagtccgctc agcaaaggca ctcaggctca accctcgctc
      961 gattcgcagt tcccgaatcc gtgtgcccat gggattccga tcgcccagtt gcttgcggtt
     1021 tgacattggc accgcacctt cccacgatct ccattcggaa cgcgctgagc actgttgtac
     1081 cgccgacgcc aacaacgaga ccgtcacgtg tcgagacggg caggaattgg cgttgcctcg
     1141 tatgccgctg atcgttggag ctggacgacc acctctgcga ggagagaatc aacatcgaat
     1201 ggcttgctga tgactgcgtc gaccggaagg ccagcggctt cggcacgtag cgtgctgctg
     1261 gccgagacgg cgataatcgg gatctcacgc gtctcgggat ttgatttcaa gattcgaata
     1321 gcatcgccac catcgacggt tggtaatcgc agatccatga ggatcaggtc ggggcgttcc
     1381 tgcagtgcca atcccaacgc tgccttccca tccagggccc gggtcaggat gtagttgtcc
     1441 tgctccatga tgaatgcgag caagtcgttg ataacaggat cgtcctcgac gatcagaatg
     1501 tgcgccatga cgtccccacc ctccccaatc agtcaagaac gccatcggat cgcagcatgg
     1561 cgttcagccg atagtagtgg cgtatgcgta acctcacact aacgtctctg cgcaactggt
     1621 gacgataccg tcagagttgg aggcgtgacg atcacgcgac acgagtttcg tgcaggtatg
     1681 cgaccgagca tcgaccgccg gatccggcgg tcggttgact ccaatggtct gttcgtctgg
     1741 ggcaacgccg cagatcttgt tcgaagagct agaccgccgc gttctccttg ccgccggccg
     1801 cctcgatccg ctcgtgccca tcgtccgggt gagcctggag cgcctgctgg cggtatttgt
     1861 ccttcagccg ctccaggaca tcatcagacg cttcttcgat atcgatcagg gagttgctgg
     1921 ccttctggac cgcgcgtaga agttcgtcga gcttgagctg gaccgcccga gcgtcgcggt
     1981 tctgcgtatt ctgaatcagg aagaccatca ggaacgtcag gacggtcgtg ccagtgttga
     2041 tgacgagttg ccacgtgtcg gaaaaaccga agaacggtcc tgagactgcc cagacgatgc
     2101 aggccagggt agagataatg aacgcccaat agtgccccat tgcggccgca gtctgggcgg
     2161 cgaagcggga gaagacttct ctcatgatgc ccccttggtg ctcgaacgta ccggcgcgct
     2221 ggcaatgctg aatcgcgcga ccgcgaaaaa tcggtcgcga ctgtgcatga tccgttccac
     2281 cccaacactc gcttgaccgg aattggcacg ctgattgcgt ttgggcgagc gaacactttc
     2341 tcgtcctggg cggtgctccc cggaagcgga gcgccacctg gtccacatac agcgaatggg
     2401 cggccggatg ggcccagccg cccgttttcc caccattcgt ttccatagtg cgacgtcgtc
     2461 gaatcgccgc cttcgacgcc aacgttgccg agcccggcgc gtcgtcctgt ccaagtagtt
     2521 gctctcctac acctaaacac agcgtgttat ccatggtccg attgttctga tggaatccat
     2581 cgaagcgcgc gtctcaaccc ccatcggacg tcatcgcgat ctcgagcacg gtcgccagcg
     2641 agaggaccga cgacgtgatg gcagtattct catcatcctg tcgaacgtca tagaagtcgg
     2701 ccctgtcgat cgttggcgtg gataccgcaa cacgcgtggg gcggtacgag atgaacagcc
     2761 gattccgatg tcatcgattg ccttcacacc gacatcgcac gcgacatgca tacgcggccg
     2821 ccaggctgat ggcgtcgccc gtgctgtagg aagcatgcgg cgacggcgtt tgcttccaat
     2881 cagcagacgc gaccgcgaca gcggaggagg tatgatggcg ctgcgcgacc agtggtacaa
     2941 gaatgcgatc gtttactgtc tcgacgtgga gacctatgcc gacgccgatg gcgacggcat
     3001 cggcgacttc cgcggtctaa cccagcggct cgactacctg tccggcctcg gtgtcacctg
     3061 tctctggctg atgccgttct acccgacccc gaaccgggac gacggctatg acgtcgccga
     3121 ctacacaacc gtcgatcccc ggttcggcac gatggcggac ttcactgact tcatggtcga
     3181 ggcacatgac cgtgggctcc gtgtcatcct cgatcttgtt cccaaccata cgtctgacca
     3241 gcacccctgg tttcaggagg cgcgtcggga cgcaaactcg ccgtaccgca actactatgt
     3301 ctggcgc
//
LOCUS       DASUGJ010000165         8953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1690069, whole genome shotgun sequence.
ACCESSION   DASUGJ010000165 DASUGJ010000000
VERSION     DASUGJ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690069"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VFV93_03600"
     CDS             complement(<1..723)
                     /locus_tag="VFV93_03600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX5164457.1"
                     /translation="MTSSLIRERLERLSVALDRQLAPKRIRMFAICVLTFWSIVHVGL
                     AATGDLPYNKFNMPILADLTNRFIGADILLNGDADRLYDLDRQTQSQRELFSQMPSDR
                     HSLFVSPPFVAYIYAPLALLPYTVSAVIWLLVSVALVFLSLRLLWPIVPDVHRHGISL
                     IMLLLFASQPMIELLGSGQDAALSLFVLAAGLRLLLSGREGLAGAVLGLGLFKPQIFV
                     FMPIFFLVTRRWRALAGWLAVAA"
     gene            complement(1259..1972)
                     /locus_tag="VFV93_03605"
     CDS             complement(1259..1972)
                     /locus_tag="VFV93_03605"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX5164458.1"
                     /translation="MSATMVAKDRRQQLLQMLPAMLLALLYASISFNKLVIVADELGR
                     LSGNDRLRHTLLALHATSSAIFFGLIAVIMFTRKQPIRREKRLIGWVLPTVVTVAMAG
                     VGWFEPRGDSTAVLLLATFFVIAGTGFTIYALRHLGRHFGVVSDVRGLVTTGPYRWMR
                     HPLYGGETLTSIGLVLAVANPVAIAAFTLGTALQITRARVEEQSLTAALPEYREYASQ
                     TPMLIPLTKIRFRPTEATA"
     gene            complement(2082..2237)
                     /locus_tag="VFV93_03610"
     CDS             complement(2082..2237)
                     /locus_tag="VFV93_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164459.1"
                     /translation="MHQRIRQSKHQGQGLVEYALILLFVAAVVVVALPAIGSALVSGY
                     ELVGAAL"
     gene            complement(2411..2587)
                     /locus_tag="VFV93_03615"
     CDS             complement(2411..2587)
                     /locus_tag="VFV93_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX5164460.1"
                     /translation="MVQYFIALLDALKTREDEGQGMVEYALILVLVSIAAVVALTAMG
                     DLIGPIFDTVSASL"
     gene            complement(2972..4072)
                     /locus_tag="VFV93_03620"
     CDS             complement(2972..4072)
                     /locus_tag="VFV93_03620"
                     /inference="COORDINATES: protein motif:HMM:NF017221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5164461.1"
                     /translation="MSMVLPPPQTREFVDRLTSLAAECRDELEQSSQSLQEIDLLLNQ
                     TQSEVDRLSQRESQQNARIREMEASLEAYSRADIRSTYLAAHDVSLRLFMMRSQLEAL
                     ESRRESIDTQQQKLRILLNLAEINREQSSDSVATNSRTTTLQGNTSLFGESAIDLAPE
                     LIQARERERLRIARQLTDGPAQVLANLILRTEIVKRAAERAPESVVEEVDDLREMAAS
                     SLLNVRRSIFEMRPLVLDELGLVPTLRRYSADFGRENGASITINGPDRDDSIAGHTRV
                     ALFRLIQQALLALVTPNTGTNVQCDIRFEEAQLAVRMDASSIGPKTVNTVAHFVEDAY
                     TLDAIDLIGGSIQHEQLPTGVRVTMIVPIGLN"
     gene            4384..4454
                     /locus_tag="VFV93_03625"
     tRNA            4384..4454
                     /locus_tag="VFV93_03625"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4416..4418,aa:Gln,seq:ttg)
     gene            4529..5977
                     /gene="glmU"
                     /locus_tag="VFV93_03630"
     CDS             4529..5977
                     /gene="glmU"
                     /locus_tag="VFV93_03630"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX5164462.1"
                     /translation="MDPDSTSQLTEPPDRQPGGVTGYGNIAVVILTAGRGTRMRSAIP
                     KVLHPIAGLPMAAHVVRAAREIAPGQIVVTIGPTSEQLRDVLTDDITFAWQPDPLGTG
                     DAARAALPHLTPAIEWVVVIFGDHPLMSDATLRMLIETTLAADPLLATVAVVLPEPGP
                     YGRYRRDSRGRVIGIVEAHEDTQTYDGPTPVNSGACCVQRDWLETAIGDIPLSPKGEY
                     YLTTLVELAARVTLPDDRDPVLLVTAPEEVALGINDRVELAEAERGIRQRINERHMRA
                     GVTLVDPEQSYIDADVTIGADTRIEPGCQLRGRTTIGAGCRIGPQSVIEDSIIHDNVT
                     IRASWIESSEVGSGVDIGPFSHLRPGATLAAGVHIGNYVELKNASIGAGTQIGHFSYV
                     GDSEVGERVNIGAGSITANYDGVAKYRTTIGNDVFIGSDTILRAPVTVGDGGRTGAGA
                     VVTKNVPAGKTVVGVPARVIGPASTPTDTGNK"
     gene            5989..6927
                     /locus_tag="VFV93_03635"
     CDS             5989..6927
                     /locus_tag="VFV93_03635"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX5164463.1"
                     /translation="MDGRLQVFAGNGNRKLAEDIMATLEAPLGRAIVDCWRNGETRVK
                     LEENVRGSDVFIIQALAEPVNHHLVELLIMIDAVRRSSASRITAVIPYFAYAKQEKKT
                     GGREPISAKLVANLLVTAGVDRVLTLDLHAPAIEGFFDIPVDHLRATPLLASAFRKMA
                     FEDIVAVSPDAGGVGRAEEFRVRSGGSLAIISKKRPAPDEAEMIEMVGDVNGKTAVIV
                     DDMISTGGTLVQAADLLIERGAARVYAAAVHGIFAGEAASLIAHSSIEKVIVTDTLPL
                     HSTAQNGSVQVLTVAPLLAEAITRIHKDLSISALFH"
     gene            6965..8290
                     /locus_tag="VFV93_03640"
     CDS             6965..8290
                     /locus_tag="VFV93_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5164464.1"
                     /translation="MSTTAITELIIAIVAIGVVGVSTWAEMSLSAGTRVDIRRLLDNR
                     LSREDALAIERIQRLRSSLLLIEMLASAVAIWMIAVALDELIEDNGLVYGLVVGLAAL
                     IIAGNLLPRVFADTEQTSEPSREIRVAKVLSFIFAPIIRPVEMFSNLLTRNKRRSGDG
                     DEADDAATDHNGNNYATGDRERGEAHDIEEDEHEMITGVLHLDEAVARDIMVPRIDII
                     AIARDVLIAEAVDVAIQHGHSRIPVYGENIDNILGVVYAKDMLRYVNEPHEGMTVEPL
                     VRPAYIVPESKRIDDLLEELQQAKVHLAVVVDEFGGTAGVVTIEDILEEIVGEIQDEY
                     DTETPPFEIVSEREAIVDGRLSIDDLADEMDMVWPEPASGTLGGYIQRRLGRIPAEGE
                     QVELPGIRLTVLLVEHRRVRQVRLERIDTGDGHAMSDGEARPAPVASKF"
     gene            8297..8869
                     /locus_tag="VFV93_03645"
     CDS             8297..8869
                     /locus_tag="VFV93_03645"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX5164465.1"
                     /translation="MNGPEQPPAGQSPWQIEWHAELPSTMTRARDLAESDAPAGTVVV
                     ADYQSEGRGTHGRIWLAPPDGCLMFTLIARPAIPIETLSDVPLRVSESVADCLRAAYG
                     VECEVKPPNDITIGGKKLAGVLCTSRLVGERLAWLLVGIGLNTRMSVMERPFETATSL
                     AIEGVAVTSHNDLLLRILDSLGWLLEPDLS"
BASE COUNT         1789 a         2786 c         2849 g         1529 t
ORIGIN      
        1 cgccgcgact gcgagccaac cagcaagcgc gcgccatcgt cgggtcacca ggaagaagat
       61 gggcatgaag acaaagatct ggggtttgaa cagaccaaga ccaaggacgg caccagcgag
      121 accctcacgt ccggacagca gcaatcgaag gccggcagcg agaacaaaca gcgagagtgc
      181 tgcgtcctgt ccggatccga gcagctcgat catcggttgg gacgcaaaca gcaggagcat
      241 gatcagactg atgccgtgtc gatgaacgtc gggcacgatc ggccacaata accgcaggct
      301 cagaaagacg agcgccacgg agacgagcag ccagatcacc gcactgaccg tgtagggtag
      361 cagcgcgagc ggcgcgtaga tgtaggcgac aaacggaggc gagacgaaca gggaatgccg
      421 atcgctcggc atttggctga agagttcacg ttgagattga gtctggcgat ctagatcgta
      481 gagacgatcg gcgtcgccat tcaggaggat gtcagcaccg ataaaccgat tcgtgagatc
      541 agccagaata ggcatgttga acttgttgta cggcagatca ccagtggcag ccaagccaac
      601 atgaacgata ctccagaacg ttagtacaca tattgcaaac atccggatgc gcttgggggc
      661 tagttgccgg tccaacgcaa cagacaaccg ctccaaacgc tcgcgaatca acgaactggt
      721 caacactcta tccatcccca aagccttcgg cgtcgcaaga ttccggagcg ttcgtcacct
      781 gttgcgacca atctcatgcc tgcctggaac cgtgggaacg ttcgctcgaa cccaaacgcg
      841 gcgaatgcat gtgacacagt acgcctccca cacgttcgtt cgagatcaag actgccgttg
      901 tactgacaat tcctcaagtg cccataggta ccgtgttggc cagcatgcac tgaggccgat
      961 gaatgtgtag tgaaccctga aagcggagag gtggtgaggt gccgttctct ccaccggcgg
     1021 tagactgcag gaaagaggag caacgcacga tgccaggggg accatcggtc gcgagttcag
     1081 attggaacag gtcgaccaga ttgaggcagg agatctcagt tggcccacgc tccaaaccaa
     1141 ggagtgcagt tcacgggaac ctgggttgtc tcacatcgac gtcgcattcg ctgacaaaag
     1201 acgacgccct ctgtatcgtg ggacaaaggg cgctgtcttt gtatccaact gcagttggtc
     1261 aggcggtcgc ttcagtgggc cggaatcgaa tcttggtcag cggaatgagc atcggtgttt
     1321 gcgaagcata ctcgcggtat tcgggtagcg cggccgtcaa tgactgctcc tccacccgcg
     1381 cgcgagtaat ctggagcgca gtcccaaggg taaatgcggc gatcgcgacc gggttggcaa
     1441 ccgcaagcac gagtccgatc gatgtgagcg tttcgccacc atacagcgga tgacgcatcc
     1501 agcgatacgg ccctgttgtc accaatccgc gcacatccga tacgacgccg aagtgtcgcc
     1561 ccaggtggcg caacgcatag atggtaaagc ccgtgccggc aatcacgaag aaggtagcca
     1621 acagcaacac cgcagtcgaa tcacctcgcg gctcgaacca gccgacaccg gccatcgcca
     1681 cggtgacgac ggtcggcaac acccaaccga tcaaacgctt ctcgcggcga atcggctgct
     1741 tgcgggtaaa catgatgacc gcaatcaggc caaagaagat cgcagacgag gtcgcatgca
     1801 gcgccaagag ggtatgtcga agccggtcat tacctgaaag gcgaccgagt tcatcagcaa
     1861 caatcaccaa tttgttgaag gagatcgacg cgtagagaag cgccagcagc atcgccggca
     1921 gcatctgtag caactgctgt cggcgatcct tggcaaccat tgttgcgctc atcaatcctc
     1981 cagactcgga actgtgttca cttagcgttt gatagactgt cggagagaac caagaataga
     2041 ccaccgaatt aacagtggtc tattcacaaa gaacagcatg actatagcgc ggcaccgacc
     2101 agttcatatc ccgacacgag agccgagcca atcgccggta gcgcgacgac aacgaccgct
     2161 gctacgaaca gcaagataag tgcgtactcg accagcccct ggccttgatg cttcgattgc
     2221 cgtatccgtt gatgcactat gactcccttc attaagaggc agggagggcc ttgctagtag
     2281 tcaactccag tcgcgatctc ctctgttcaa agcggcgtga taccggcgag atcttgacat
     2341 gacctatgca aaaagggtaa cgcgcctcga ctcatgaggc gcgttacccc ttaactgcct
     2401 cggaagtgaa ctacaggctg gcggagaccg tatcgaagat cgggccgatg aggtcaccca
     2461 tggccgtcaa ggcgacgacg gcggcgatcg agacgaggac caggatcagc gcgtactcga
     2521 ccataccctg tccctcatcc tcgcgggtct tgagggcgtc caggagagcg atgaaatact
     2581 gaaccacaat gggctccttc cgaaatgcgg caattactgc cagataatca gacctcagca
     2641 acatactgga cgagaccgcc gggtcagttc gtatctcgtc aggtctttcg ttctccctgt
     2701 acggttttcc gaagacattc agagcatagg gcacattccc tggttctcaa atgggtcaaa
     2761 atccccaacc gcatgcggtt ctgcggtacc ccggtaccat gccgcgagta cccttcagat
     2821 ggtcccaggg catgctgcat ctgaacggca accagcggga gatgccgacc tcgtcggaga
     2881 ggccgatctc ccggcagata ccgcaaacac cgagcggctg ccacacgcga tggcagccgc
     2941 tcggtcggaa agcgtggacg tggtgtgggt gctagttcag gccgatcggg acgatcatcg
     3001 tgacgcggac gccggttggg agctgctcat gctggattga gccaccgatc aggtcgatcg
     3061 cgtcgagcgt ataggcgtcc tcgacgaagt gagcgacggt gttcacggtc ttcgggccga
     3121 tgctggaggc atccatccgg acagccagct gcgcctcttc gaagcggata tcgcactgga
     3181 cgttcgtgcc ggtgttgggc gtcaccagtg ccagcagcgc ctgctggatc agccggaaga
     3241 gggcgacacg ggtgtggccg gcgatcgagt cgtcgcggtc cgggccgttg atcgtgatcg
     3301 aggcgccgtt ctcgcggccg aagtcggccg agtagcgccg cagggtcggg acaagcccca
     3361 gctcgtccag gacgagcggg cgcatctcga agatcgagcg gcggacgttg agtagcgaac
     3421 tggcggccat ctcacgcaga tcgtcgactt cctcgacaac cgactccggc gcgcgttcgg
     3481 cggcgcgctt gacgatctcg gtccgcagga tcaagttggc cagcacctgg gccggaccgt
     3541 cggtgagctg cctggcgatt cgcaaccgct cgcgctcgcg cgcctggatc agttccggcg
     3601 ccaggtcgat ggccgattca ccaaacaggc tcgtgttgcc ctggagtgtc gtcgtccgcg
     3661 agttggtcgc aaccgagtcg gatgactgct cgcggttgat ctcggccagg ttaagcagga
     3721 tgcgaagctt ctgctgctgg gtgtcgatcg actcgcggcg cgattcgagc gcctccagct
     3781 ggctgcgcat catgaacagc cgcaacgaga catcgtgggc ggccaggtag gtgctgcgga
     3841 tgtcagcccg cgaataggct tcgaggctgg cttccatctc acggatgcgc gcgttctgct
     3901 gcgattcccg ctgcgaaagc cggtcgacct cagactgagt ctggttcagt aggagatcga
     3961 tctcctgcaa cgactggctg ctctgctcca gctcgtcgcg acattcggcc gccagcgatg
     4021 tcagccgatc gacgaactcg cgggtctgag gagggggtaa gaccatcgac atactgtatc
     4081 tccatgccct gtccggaact gccggatcga cgacgggcga acgatggcgc actccggcgc
     4141 caattccctc caccgatcat agcctgccgg gaatcgcggg cgtaccctcc caagggtccc
     4201 gaaagtcatg gttttgtgat gggtaacgcc gggattcggg acacgggatt cggggctgaa
     4261 ggaagtttcg gttccaacat ggactacggc tgtgtcccag ccccgaatcc cgagtcccga
     4321 atcccggcac cacggcaacg cattgcccgc tgatgggcac attcgttaca cttgaaactg
     4381 cggtcgcggg tggtgaaatg gtatcacagg ggtctttgga acccttattt caggttcgag
     4441 tcctggcccg cgagcttctc gccaatctgg cgtgaggagc ctcgcgcagg gagccgtaac
     4501 cgtgctgcag tcgtggtgat agggacgctt ggacccggat agtaccagcc aactcactga
     4561 acctcccgac cgccagcccg gcggtgtgac cggctacggc aatatcgccg tcgtcatcct
     4621 cacggccggg cgtggaaccc ggatgcgctc tgcgatcccg aaggtgcttc atccgatcgc
     4681 cggcctgccg atggccgcgc atgtcgttcg cgccgcccgc gagatcgccc ccggccagat
     4741 cgtcgtgacg atcggcccaa cctccgaaca gcttcgcgac gtgctgaccg acgatatcac
     4801 gttcgcctgg cagcccgacc cgctcgggac cggcgacgcg gctcgtgctg ccctgccgca
     4861 cctgacgccc gccattgagt gggtcgtcgt catcttcggt gaccacccgc tgatgtcgga
     4921 cgcaacgctc aggatgctga tcgagacgac gctggcggct gatccgctgc tggcgacggt
     4981 cgcggttgtc ctgccggagc cggggccgta cggtcgctac cggcgcgact cgcgcggccg
     5041 agtgatcggc atcgtcgagg cgcatgagga cacgcagacg tacgatgggc cgacgccggt
     5101 caactccggc gcctgctgcg tccagcgcga ctggctggag acggcgattg gcgacatccc
     5161 gctgtcgccg aagggcgagt actacctgac aacgctggtc gaactggccg ccagggtgac
     5221 actgccggac gatcgcgacc cggtcctgct ggtaacggcg cccgaggaag tcgcgctcgg
     5281 catcaacgac cgtgtcgagc tggccgaggc cgaacggggt atccggcagc ggatcaacga
     5341 acgccacatg cgcgctgggg taacgctggt cgatcccgag cagagctaca tcgacgccga
     5401 tgtcacgatc ggcgccgaca ctcggatcga gccgggctgc cagttgcgcg ggcggacgac
     5461 gatcggcgcc ggctgccgga tcgggccgca gagcgtcatc gaggattcga tcatccacga
     5521 caacgtcact attcgcgcct catggattga gtcctccgag gtcggcagcg gcgtcgatat
     5581 cgggccgttc tcccatctcc ggccaggggc gacgctcgcg gccggggttc acatcggtaa
     5641 ctacgtcgaa ctgaagaacg cgtcgatcgg cgccgggacg cagatcggcc atttctcgta
     5701 cgttggcgac tctgaggtcg gcgagcgggt gaacatcggg gccggctcga tcacggccaa
     5761 ctacgacggc gtcgccaagt accgcacgac aatcggcaac gacgtcttca tcggcagcga
     5821 cacgatcctt cgggcgccgg tgacggtcgg cgacggcggc cgaaccgggg ccggcgcggt
     5881 cgtcacgaag aacgtcccgg ctgggaagac ggttgtcggc gtcccggcgc gggtgatcgg
     5941 cccggcaagc acacccaccg atactggcaa caagtagaga caggacagat ggacggacga
     6001 ctacaggtct tcgcgggaaa cggcaatcgc aagctggccg aggacatcat ggcgacgctc
     6061 gaagcgcccc tcggccgggc gattgtggac tgctggcgca acggcgagac gcgcgtcaag
     6121 ctcgaagaga acgtgcgcgg ctcggatgtc ttcatcattc aggcgctggc cgagccggtc
     6181 aaccaccatc tggtcgagct actgatcatg attgacgccg tccgtcgctc gtcggcgtcg
     6241 cgcatcacgg ctgtcatccc ctacttcgcc tacgccaagc aggagaagaa gacgggcgga
     6301 cgggaaccga tcagcgccaa gctggtcgcg aatctgctgg tcacggccgg cgttgaccgt
     6361 gtcctgacgc tggatctgca cgccccggcc atcgagggct tcttcgatat cccggtcgac
     6421 cacctgcggg cgacgccact gttggcgtcg gcctttcgca agatggcgtt cgaggacatc
     6481 gtcgccgtca gcccggacgc cggcggcgtc ggccgggccg aggagttccg cgtccgctcg
     6541 ggcgggtcgc tggcgatcat ctccaagaag cgaccggcac ccgatgaggc cgagatgatc
     6601 gagatggtcg gcgatgtcaa cggcaagaca gccgtcatcg ttgacgacat gatctcgacc
     6661 ggcgggacac tcgttcaggc cgccgacttg ttgattgagc gcggcgcggc tcgcgtctac
     6721 gccgccgccg tccacggtat cttcgccggc gaggcggcca gcctgatcgc ccactcgtcc
     6781 atcgaaaagg tcatcgtgac cgacacgcta ccgttgcatt cgaccgccca gaacgggagc
     6841 gtccaggtcc tgacggttgc gccgctgctg gccgaggcca tcacgcggat tcacaaggac
     6901 ctctcgatca gcgcgctgtt ccattgagga cgatagcgcg accgtgccga gcctaggagt
     6961 gtgattgagt accaccgcca tcaccgagct gatcatcgcc atcgttgcca tcggggtcgt
     7021 cggggtatcg acctgggcgg aaatgagcct ctcagctggg acccgcgtcg atattcgccg
     7081 actactcgac aatcgactct cacgcgagga cgcgctcgcg atcgagcgga ttcaacggct
     7141 gcgctcgtcg ttgctgctga tcgagatgct ggcatcggcc gtcgcgatct ggatgatcgc
     7201 cgtcgcgctg gacgaattga tcgaggacaa cggcctggtc tacgggctgg tggtcgggct
     7261 ggcggcgttg atcatcgccg gcaacttgct gccgcgcgtc ttcgccgaca ccgagcagac
     7321 cagcgagccg tcacgcgaga tccgcgtcgc gaaagtgctc tcgttcatct ttgcaccgat
     7381 catccggccg gtcgagatgt tcagcaacct gctgacccgc aacaagcgcc gctcgggcga
     7441 cggcgacgag gcggatgatg ccgccaccga ccacaacggt aacaactatg cgaccggcga
     7501 ccgcgagcgg ggcgaagcgc acgacatcga ggaggacgag cacgagatga tcaccggcgt
     7561 cctccatctg gatgaggccg tcgcgcggga catcatggtg ccacggatcg acatcatcgc
     7621 catcgcccgc gacgtgctga tcgcggaagc ggtcgatgtc gccatccagc acggacattc
     7681 gcggataccg gtctacggtg agaacatcga caacatcctc ggtgttgtct acgccaagga
     7741 catgctgcgc tacgtcaatg agccgcacga ggggatgaca gtcgagccgc tggtccggcc
     7801 cgcctacatc gtgccggagt cgaagcggat cgacgacctg cttgaggagc tacagcaggc
     7861 caaggtccac ctggccgtcg tcgtggacga gttcggcggg acagccggcg tcgtgacgat
     7921 cgaggacatc ctcgaagaga tcgtcggcga gattcaggac gagtacgaca ccgagacgcc
     7981 gccgttcgag atcgtctcag agcgggaggc aatcgtcgat ggccgcctca gcatcgacga
     8041 tctggccgac gagatggaca tggtctggcc ggagccggcc agcggcacgc tcggcggcta
     8101 cattcagcgc cggctgggcc ggatcccggc cgagggcgag caggtcgagc tgcccggcat
     8161 ccggctgacg gtcctgctgg tcgagcatcg ccgcgttcgc caggtgcgcc tcgaacggat
     8221 cgacaccggc gacggccacg ccatgtcgga cggcgaggct cgcccggccc cggtcgccag
     8281 caagttctga ccggcgttga acggtcccga acagccgccg gccggccaat cgccctggca
     8341 gatcgaatgg cacgcggaac tgccatcgac gatgacccgc gcccgcgacc tggccgaatc
     8401 cgacgcgccg gccgggaccg tcgtcgtcgc cgattaccag agcgaggggc gcggcaccca
     8461 tggccggatc tggctggccc cgcccgatgg ctgcctgatg ttcacgttga tcgcccgtcc
     8521 cgccatcccc atcgagacat tgagcgacgt tccgctgcga gtcagcgaat cggttgccga
     8581 ttgcctgcgc gcggcgtacg gtgtcgaatg cgaggtcaag ccgcccaacg acatcacgat
     8641 cggcggcaag aagctggccg gcgtcctctg cacctcacgg ctggtcggtg agcggctggc
     8701 atggctgctg gtcgggatcg gcctcaacac gcggatgagc gtgatggagc ggccgttcga
     8761 aaccgccacg agcctggcga tcgaaggcgt cgcggttacc agccacaacg acctgctgct
     8821 gcggatactc gacagcctgg gctggctgct cgaacctgat ctgtcctgac cgctggttgc
     8881 tgcacgcgct ggacggacgg cgactcgcgc tgggcctttg cctggcgatt acctgacaga
     8941 cgattccaat cgt
//
LOCUS       DASUGJ010000166         4504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_161453, whole genome shotgun sequence.
ACCESSION   DASUGJ010000166 DASUGJ010000000
VERSION     DASUGJ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161453"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VFV93_03650"
     CDS             <1..1497
                     /locus_tag="VFV93_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164466.1"
                     /translation="TGRVLGVDAATADQVIQFRSDLSGNSLESMLGELEADRPEVPMV
                     ELPGEPLRLALDIDVELDSLPGDAPAEANQPIAPFVSVVIQDARGMLYRLSGGSLESD
                     AGVQQLVIDLGFDLGESEARPEYPINLVAIEARILIPIQVPRTGRFELVTLQSSASVE
                     GDDWLPVQIASDPARWEFQTSLVTGLQDAPSIEGAAVDGDGSVAFAFTTGSTENANRI
                     LPLVYSVQPAADGEPDAQTPIAAIVSDSFLAATEGRVGDQLQVDIAGSRRPLRIVGAV
                     KAFPTVEPDSGPLILVDFPTYSALQFQTSGRIDAADEWWLATDNDETESIAATLGAAP
                     YSSWRINDRFSQAELLRTDPVALGIIGALSLGFVSAALFAVIGFVVSAAVSAHERLTE
                     FSLLRALGLSPRQLSGWLSLENGLLVLISLIGGTALGLLMAWMVLPFVTLTQDASTVV
                     PGIIVAIPWGSILLLEGITIGALALVVLALAFLLRRVGLGTVLRLGEE"
     gene            1501..4359
                     /locus_tag="VFV93_03655"
     CDS             1501..4359
                     /locus_tag="VFV93_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5164467.1"
                     /translation="MARAAAQPRSTGFPFAPETVRALLRRLRAERDLLAVVVGVVLVT
                     TFVFAAIPRLFNEMADDGLAHAVTTSNPLMRNLQMTQPGRIEVAKSGDDPFALVDQVG
                     QEFQDGLAPSIQQIIGDRKFVIDTPRYRVFQTPGVQEYPFPRYITMRYHQDVDPHLTL
                     IAGHMPQPRSDTLAVSGPLTRDETVDAPIFEVAVSTETARQLAVEVGEQLVMEPFTED
                     RLVRSVPRSQQNYVVMEISGLFEVNDVDEEYWLSIPTIDRATEYDDGNQVHIYATGVM
                     AAGAYQQYLTDTTFPLTYAWRYFVDPASFDAGDLTQLAADVRRLEAEYSTFSGAGIGE
                     TGVTTGLTTIFRRYLSQREVTEAVLSLASIGLLAVAFAVIGLVATLVAERRRQMVALV
                     RGRGGSVGQLAGAQVVEGLLLTIPSALLGLLLASVIVAGRFSIWSIWAVVVIAGVTTL
                     LLVTAALPIIRQGLGAAERSEVPVTRVSLRRVVLDLLIVALALVGVYLLRRRGLTGDS
                     SSDEINEWDPYLAAVPVLLGLATGLVVLRLYPLPVRLFGWAAGLRRDLVPALGFRRLG
                     RQPGSSNLPLLVMLLAVAVGVLSSIMLYTIGSGQTESSWQLVGADYRVDAASGYLVPS
                     IDLSEVSGVEAIATSYMLPDVKFANRSPIIGTTYFHALDATTYEEVTRGTPADPHLPG
                     ELLDEPRGTDFGQPTNPIPAVVSDHFSRNQLRPGDTFALTVFGRETTFVVVESRERFA
                     GIPIGRPFVVTSLELLEASNPDRMFRPTAMFLRAPESAFDAIDTTLTSQSISARLTSR
                     DKEYAKVHDSPLISGVEGGFKIGLVLAGAYSALAVVIALTITAHARARDLAFLRTMGL
                     SREQAIGLTIVEQLPLVVLALVAGTLLGVAVARLIEPGVDLQAFTGEGLPVELRINWV
                     NIGLLGLGLVATVAIAVAVTSSLARRASLGQALRLGDE"
BASE COUNT          659 a         1366 c         1635 g          844 t
ORIGIN      
        1 acgggccggg tgctcggggt ggacgcggcg accgcggatc aggtcatcca gttccgctcg
       61 gatctctcag gcaattcgct ggagtcgatg ctgggcgagc tggaggccga tcggcctgag
      121 gtgccaatgg tggaactgcc cggcgagccg ttgcggctgg cgcttgatat cgacgttgag
      181 ctggactcgt tgccgggaga cgcgccagcg gaggcgaatc agccgattgc tccatttgta
      241 tcggtcgtta ttcaggatgc gcgcgggatg ctctaccggc tctcgggcgg gtcgctggag
      301 tcggacgctg gtgtccagca gctggtgatc gatctggggt tcgatctggg cgagagcgag
      361 gcaaggccgg agtatccgat caacctcgtc gccatcgagg caaggattct gataccgata
      421 caggtgccgc gaacgggccg tttcgagttg gtgacgttgc agtcgagtgc ttcggttgaa
      481 ggggatgact ggctgccggt gcaaatagcg agcgacccgg cacgctggga gttccaaacg
      541 tcgctcgtga ccggtctgca ggacgcgccg tccattgagg gtgcggcggt ggatggtgac
      601 gggtcagtgg cgttcgcctt cacgacgggt tcgacggaga atgccaaccg gatcctgccg
      661 ctcgtctact cggtccagcc ggcggctgac ggcgagccgg atgcgcagac gccgatcgcg
      721 gccatcgtca gcgactcgtt cctggcggca accgaggggc gagttggcga ccagctccag
      781 gtcgacattg ccggatcgcg gcggccgttg cggatcgtcg gggccgtcaa agcgtttccg
      841 acggtcgaac cggattcggg accgctgata ctcgtcgatt tcccaaccta ctcggcgttg
      901 cagttccaga cgagcggccg gattgacgcg gccgacgagt ggtggctggc gaccgacaac
      961 gacgagacgg agtcgatcgc ggcgacgctc ggcgcggcgc cgtattcgag ctggaggatc
     1021 aacgaccgct tctcgcaggc tgaactgctg cggaccgatc cggtcgcgct cggcatcatc
     1081 ggcgcgctgt cgctgggatt cgtctcggcg gcgttgttcg ccgtgatcgg gttcgtcgtc
     1141 agcgcggccg tctcggccca cgagcggctg accgagttct cgctgctgcg ggcactgggc
     1201 ctgtctcccc gccagctctc cggctggctg tcgctggaga acgggctgct ggtgctcatc
     1261 agcctgatcg gcgggacggc gctggggttg ctaatggcgt ggatggtgtt gccgttcgta
     1321 acgctgacgc aggacgcgtc gacggtcgtg ccagggatca tcgtcgcgat cccatggggc
     1381 agcatcctgc tgctcgaagg gatcacgatc ggggcgttgg cgttggtggt gctggcgctg
     1441 gcgttcctgc tgcggcgggt cggacttggg accgttctgc ggctggggga ggagtagccg
     1501 atggcacgcg ctgccgccca gccacgttcg acggggttcc cgttcgcgcc ggagacggtc
     1561 cgggcgctgc tacgccggct gcgggccgag cgcgacctgc tggcggtcgt cgttggggtc
     1621 gtgctggtga cgacctttgt cttcgcggcg attccccgct tgttcaacga gatggccgac
     1681 gacggcctcg cccatgcggt gacgacgtcc aacccgctga tgcgcaacct gcagatgacg
     1741 cagcccggac ggatcgaggt cgccaagtcc ggtgatgatc cgttcgcgct ggttgatcag
     1801 gtggggcagg agttccagga tgggctggct ccctcaatcc agcagatcat cggggatcgc
     1861 aagttcgtga tcgatacacc gcgctaccgg gtgttccaga cgccgggcgt gcaggaatat
     1921 ccgttcccac gctacatcac gatgcgctat caccaggacg ttgacccgca cctgacactg
     1981 atcgccggcc acatgccgca accgcggtcc gatacgctgg cagtcagtgg cccgctgacg
     2041 cgggatgaga cggtcgatgc gccgatcttc gaagttgctg tctcaaccga gaccgcccgg
     2101 caactggcgg tcgaggtcgg ggagcaactg gtgatggagc cgttcacgga agaccgcctg
     2161 gtgcggtcgg tgcctcgtag ccagcagaac tacgtcgtga tggagatcag cggactcttc
     2221 gaggtcaacg acgtcgacga ggagtactgg ctgagcattc cgacgatcga ccgggccacc
     2281 gagtacgacg acggcaatca ggtccacatc tacgcaacgg gcgtaatggc ggccggcgcc
     2341 taccagcagt acttgacgga caccaccttc ccgctaacgt acgcctggcg ctatttcgtc
     2401 gatccggcgt cgttcgatgc cggcgatctg acccagctcg cggccgatgt gcggcggctg
     2461 gaggcggagt actcgacctt cagcggggcc ggtatcggcg agacgggcgt gacgaccggg
     2521 ctgacgacga ttttccggcg ctacctgtcg cagcgcgaag tgacagaagc ggtgttgtca
     2581 ctggcgtcaa tcgggctact ggcggtagcg ttcgccgtaa tcgggctggt ggcgacgctg
     2641 gtggccgaac gcaggcggca gatggtagcc ctcgtgcgcg gccgtggcgg ctcggtcggc
     2701 cagctggccg gggcgcaggt cgtcgagggg ctgctgctga cgatcccgtc cgcactgctg
     2761 gggctgctgt tggcgtctgt catcgttgcc ggccgcttca gcatctggtc gatctgggcc
     2821 gtcgtggtga tcgccggcgt gacgacactg ttgctggtga cggccgcgct gccgatcatc
     2881 cggcaggggc tgggcgcggc cgagcgctcc gaagtgccgg tcacgcgggt gtcgttgcgg
     2941 cgggtggtgc tggatctcct gatcgtcgcg ctggcgctgg tcggcgtcta cctgctgcgc
     3001 cggcgcggac tgaccggcga cagctcgtcg gacgagatca acgagtggga cccgtacctg
     3061 gcggcggtgc cggtcttgct ggggctggcg acgggactgg tcgttctgcg gctgtatcca
     3121 ctgccggtgc ggctgttcgg ctgggcggcg gggttgcggc gcgatctcgt gccggcgctg
     3181 ggcttccggc ggctcgggcg gcagccgggg tcatccaacc tgccgctgct ggtgatgctg
     3241 ctggcggttg cggtcggcgt cctgtcgtcg atcatgctct acacgatcgg cagtgggcag
     3301 accgagtcgt cgtggcaact ggtcggcgcc gactaccggg tcgatgcggc gtcgggctat
     3361 ttggtgccat cgatcgatct gtccgaggtt tccggcgttg aggcgatcgc gacctcgtac
     3421 atgctgccgg atgtcaagtt cgccaaccgc tcgccgatta tcggcacgac ctacttccac
     3481 gcgctcgacg caaccactta cgaagaggtg acgcgcggca cgccggccga tccgcacctg
     3541 ccgggcgagt tgctggacga gccgcgcggg acggacttcg gccagccaac gaacccgatt
     3601 ccggcagtcg tgtccgatca tttctcgcgc aaccagctcc ggccgggcga cacgttcgcg
     3661 ctgaccgtct tcgggcgcga gacgaccttc gtcgtcgtcg agagccgcga gcggtttgcc
     3721 ggcatcccga ttggccggcc gttcgtcgtg acctcgctgg agctgctgga ggcgtcaaat
     3781 ccggaccgga tgttccggcc gacggcgatg ttcctgcgcg cgccggagag cgcgtttgac
     3841 gcgatcgaca cgacgctcac gtcgcaatcc atctcggcac ggctgacgtc gcgcgacaag
     3901 gagtacgcga aggtccacga ctcgccgctg atctccggcg ttgagggagg gttcaagatc
     3961 gggctcgtgc tggccggggc gtactccgcg ctggcggtcg tgatcgcgtt gacgatcacg
     4021 gcccacgccc gcgcacgcga tctggccttc ctgcggacga tggggctatc ccgcgagcag
     4081 gcgattgggc tgacgatcgt cgagcagcta ccgctcgtcg tgctcgcgct ggtggccggg
     4141 acgctgctcg gcgtggccgt cgcgcggctg atcgagccgg gggttgacct acaggcgttt
     4201 accggagaag gtctgccggt cgaactacgc atcaactggg tcaacatcgg cttgctcggg
     4261 ctcggcttgg tcgcaaccgt cgcaatcgcg gtcgccgtca cctcgtcgct ggcacggcgg
     4321 gcgagcctgg ggcaggcgtt gcggttgggg gatgagtgat ggacggcggg attcgggata
     4381 cgggaatcgg ggttggtcaa ccaagcgcta tgattggcag atcgattgtt caactgcccc
     4441 gaatcccgaa tcccgacgat atgcgtacac tgttgtgtta gcggcatggc tacacgaggg
     4501 ggct
//
LOCUS       DASUGJ010000167         7403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1690472, whole genome shotgun sequence.
ACCESSION   DASUGJ010000167 DASUGJ010000000
VERSION     DASUGJ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690472"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1737)
                     /locus_tag="VFV93_03660"
     CDS             complement(61..1737)
                     /locus_tag="VFV93_03660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX5164468.1"
                     /translation="MARPRMRAIPLGGIGEVGKNMMIVEYRGDMVMIDSGTKFPEEEM
                     RGIDLIIPDITYVIDNIQRFRAILITHGHEDHIGSLPYVLPQLERIAPVPIYGSPLAL
                     AFARSRLDEARNAHLADFMPIEPGIVYSIGQHMEAEFIPVTHSIPGSMAVTLKTPVGR
                     MIHTGDFKFDPTPPLGPRTDADRLRELGDEGVLALFSDTVRIERKDATPSESTVSATL
                     DRIIGGATGRVILTSFASNILRLEQAIQVGAKYGRKVGVVGRSMEQSVKVAIDLGYIQ
                     PPIGTMLPTDEVIKLPKDQILILTTGSQGEASAALARMASGDHPLLRIQEGDTVILSA
                     TPVPGNEETVAQTIDNLFKRGASVCYSAIEPSIHVSGHGGADELRQMIELVRPTFCIP
                     IHGEYRHLHLYQQLAIDAGIPPENTMIPESGVPVSFSRNDMRKDPPVPYGAVLVDGLG
                     TDRYRNVVLRHRDHLANTQVVIATVIVDLTRGVLIAGPELTGKGFDDEDSRELIENAE
                     RELKNYLERQLRKGGMSYGYLVSRVKDTVARYIFQKAKLRPMILPVVTEL"
     gene            2005..2205
                     /locus_tag="VFV93_03665"
     CDS             2005..2205
                     /locus_tag="VFV93_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164469.1"
                     /translation="MSQVNVDPGGDRGGSGVNAVAVVAIVILVILAFLAVYFLFLGDD
                     GDADINVDVGSPTSLVRGGFNG"
     gene            2332..2976
                     /locus_tag="VFV93_03670"
     CDS             2332..2976
                     /locus_tag="VFV93_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164470.1"
                     /translation="MSDENDRQPNEQARTEATGDDQFLAERVHADRPAQTSDSEPARQ
                     PWRPGEPLTAKIRETVFKRPGTREQVPATEATGADLSEVNATTVLMNQSGSEGITADR
                     VTMERSGARAIEAKSVQMDQSGVVALSTESAVLLKSSAVQVVAEKARLSNSAALFLSS
                     GEASIDNSRIAIFAGSADGDVQALITAKTAAILAGVLAVFLALLVALPRNRTRD"
     gene            3006..3263
                     /locus_tag="VFV93_03675"
     CDS             3006..3263
                     /locus_tag="VFV93_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164471.1"
                     /translation="MNNGTKLIVGSLLGAGVGYIVSKVMEREVVEPVSFTDTPPEPKE
                     TFKERWQRAKLAGEEARLAKEAELRAYFRTKVNDPNALTTE"
     gene            3495..4184
                     /locus_tag="VFV93_03680"
     CDS             3495..4184
                     /locus_tag="VFV93_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5164472.1"
                     /translation="MENVSSASIGDMLVQSIDTISPDETVTVAKRRMESQTSRSLIVV
                     DDNRPVGVVQWRGLNRREGSELIRDVMQTEFPILRSGMSVSDVRDYLSGVDVDLDHLP
                     VVDESGMLLGEVPRGVITKGETATNAATEAVVAGPDADPRRDSQPQVRLEQGMKVIDA
                     NGKSLGSIDQVELSAEGQIAHFAVKYGMLGRNSKRLPADVISRVEGNEVHVNIGQPEF
                     KMLADVGEVVI"
     gene            complement(4258..4812)
                     /locus_tag="VFV93_03685"
     CDS             complement(4258..4812)
                     /locus_tag="VFV93_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164473.1"
                     /translation="MNTQESGTQHNAIPPLPVEDERRGFRIHPGTIVAVLGVLLIGLI
                     AIGIFVNRESEPGVEATTIAELRADPTGWDNRRVTLTGTAESVRELPILSQYAIYTFR
                     DETGSMLALTRNGAPPSDGQRLQVEAVFHSKVRLDDELKEIVSDQLGPLAGSVVSTLV
                     PGVPLDVVYLGHDSYDVLGTPEGG"
     gene            complement(4914..5414)
                     /locus_tag="VFV93_03690"
     CDS             complement(4914..5414)
                     /locus_tag="VFV93_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5164474.1"
                     /translation="MTSRTMTFSPWADFAYLQHQLDRLAQDAFGLRSQTSITTLPVDV
                     FDKGEQLIVQAFVPGLRAEHVDIQVEDNTVTISGQFPQLYDAEESRGYTWYARELRGG
                     RFGRSINLPYKVDWDNTVATVADGLLTLTFPKAPEARPRRIQIREASSMSDTPELTAT
                     SGGMES"
     gene            complement(5670..6587)
                     /locus_tag="VFV93_03695"
     CDS             complement(5670..6587)
                     /locus_tag="VFV93_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5164475.1"
                     /translation="MPVQISDEFHRRIAGVFGDAGLGWLARLPEIVAECAEQWGLVVE
                     PPFALSYNYVAPARRADGTPVVLKVRCPHEEASAEMEALRAFAGDGICAVLAQDEERN
                     ASLLERVLPGMPLAALVAEDDERATAVAIDLMQRLWRPPPVGHRFPTVADWAAGLARH
                     RERFDGGTGPLPARLFEEAEDAFAWLLATTAEALLLHGDLHHANILSATRQPWLIIDP
                     KGIVGDPGYDLGAFLYNPMPGLLDLPNPGQIITRRVDQLAEGLGMERARVRGWGIAQA
                     VLSACWSIEGDEDWRHTIAVAEYLAALAE"
     gene            complement(6681..6971)
                     /locus_tag="VFV93_03700"
     CDS             complement(6681..6971)
                     /locus_tag="VFV93_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164476.1"
                     /translation="MAQIARSIEIDIDWLLDFLTGEWRNVESVALAWDTLDQEARLDY
                     LNEWPITESYGDVLDDAASHQRLTPDQDIRYQQLQHLIRKLRPTIEHLLAIG"
     gene            complement(6959..>7403)
                     /locus_tag="VFV93_03705"
     CDS             complement(6959..>7403)
                     /locus_tag="VFV93_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164477.1"
                     /translation="DDIDAIIEHVATASFSAKQWSVPWQMRIVFHGVRIGPTAESALY
                     HVLKRVVHDRQWSIGTSLSDYLTDVSSAVRSPGARIAIYRRWNQTTVAFLSETLVTVP
                     SHRLGSAALPFTLVLYSADTGKISTAHMVVSETAARIPGHARWLR"
BASE COUNT         1350 a         2336 c         2406 g         1311 t
ORIGIN      
        1 cgcttgggcg aggaaccagg gccggcacaa ggcacggccc ctacttctgt gcggcgacga
       61 ttacagctcc gtcacgaccg gcaggatcat tgggcgtagc ttggccttct ggaagatgta
      121 gcgggcgacg gtgtccttga cgcgcgagac gaggtagccg taggacatgc cgcccttgcg
      181 gagctggcgt tcgaggtagt tcttcagctc ccgctcggcg ttctcgatca gctcgcggct
      241 gtcctcgtca tcgaagccct tgccggtcag ctccgggccg gcgattagaa cgcctcgtgt
      301 cagatcgacg atcacggtcg cgatgaccac ctgcgtgttg gcgagatggt cgcggtggcg
      361 caggacgacg ttgcggtagc ggtcggtgcc gaggccatcg accaggaccg cgccgtacgg
      421 caccggcggg tctttgcgca tgtcgttgcg cgagaaggag accggcacgc cggattcggg
      481 aatcatcgtg ttctcgggcg ggatgccggc gtcaatcgcc aattgctggt agaggtgcag
      541 gtggcgatac tcgccgtgga tcgggatgca gaacgtcgga cggaccagct cgatcatctg
      601 gcgcagctcg tcggcgccgc cgtgacccga gacatggatg ctcggctcga tcgccgagta
      661 gcagacgctc gcaccgcgct tgaacaggtt gtcgatcgtc tgggcgaccg tctcctcgtt
      721 gcccggcacc ggcgtcgccg agaggatgac ggtgtcgcct tcttgaatcc gcagcagcgg
      781 gtgatcgccg ctggccatcc gcgccagcgc cgccgaggcc tctccctgcg agccggtcgt
      841 caggatcagg atctggtcct tcggcagctt gatgacctcg tcggtcggca gcatcgtacc
      901 gatcggcggc tgaatgtagc cgaggtcgat cgcgaccttg acggattgct ccatgctgcg
      961 gccgacgacg ccgaccttgc ggccatactt cgcgccaacc tggatcgcct gctccagccg
     1021 gaggatgttc gaggcaaacg aggtcaggat gacccggccg gtcgcgccgc cgatgatccg
     1081 atccagcgtc gccgagacgg tggactcgct cggcgtcgcg tccttgcgct cgatccggac
     1141 cgtgtcggag aagagggcca gaacgccctc gtcgccaagc tcgcgcagcc ggtcagcgtc
     1201 ggtacgcggt ccgagcggcg gggtcggatc gaacttgaag tcaccggtgt ggatcatccg
     1261 gccgaccggc gtcttgagcg tcaccgccat cgagccggga atcgagtggg tgaccgggat
     1321 gaactcggcc tccatatgct ggccgatgct gtaaacgatg cctggctcaa tcggcatgaa
     1381 gtcggccaga tgcgcgttgc gagcctcgtc gagccgcgag cgggcgaacg ccagcgccag
     1441 cggtgagccg tagatcggga ccggcgcgat ccgctccagt tgcgggagca catacggaag
     1501 cgagccgata tggtcctcgt gtccgtgggt aatcaggatt gctctgaagc gctggatgtt
     1561 gtcgatcacg taggtgatgt cggggatgat gaggtcgatg ccgcgcatct cttcttctgg
     1621 gaacttggtg ccggagtcga tcatgaccat gtcgccgcgg tattcgacga tcatcatgtt
     1681 cttgccgact tcgccaatac cgcccagcgg gatagccctc attcgcggtc gggccatagt
     1741 ttcgagttcc tccttcacgg cacatcttgg gccgatggtg tcaactgtcg tgtgcgtgcc
     1801 acgtcgtcat gttgcggcaa ggataccgcg ccacgcatag cgcccgttgc gaaccaccac
     1861 gcacggccaa tgccccgccg gccacgcctg agccatgtct tgcgtggcac ggtgtttgcc
     1921 tcccttgcgc caacgacagg ctgtcggcgg cggcgtcgct cgctgatgtc gccggctgcg
     1981 gtcaagcaat aggaggtaca ggacgtgtca caagtcaacg tcgatccagg tggtgatcgt
     2041 ggcggaagtg gtgtcaacgc cgtcgccgtc gtcgcaatcg tgattctggt catccttgca
     2101 ttcctcgccg tctatttcct cttcctcggt gacgacggcg acgctgatat caacgtcgat
     2161 gtcggctcgc caaccagcct ggtgcgcggc ggattcaacg gttagccgcg gcgggcggcg
     2221 ttgacggacg ctgcccggct ccgtaagatg cgatggttca cggggcgtgg accatcgcat
     2281 cgtgctgtcc gggacatgaa cgccgcgaca tccgggactg gggattgagc catgagcgac
     2341 gagaatgacc gccagcccaa cgagcaggcc aggactgagg caactggcga cgatcagttc
     2401 ctcgccgaac gggtccacgc cgatcggccg gcccagacga gcgactccga accggcccgc
     2461 cagccgtggc ggccgggtga gccgctgacg gccaagatcc gcgagacggt cttcaagcgc
     2521 cccggcaccc gcgagcaggt gccggcaacc gaggcgaccg gcgccgacct gagcgaggtc
     2581 aacgcgacga cggtcctgat gaaccagtcc ggttcggagg gcatcaccgc cgaccgcgtc
     2641 acgatggagc gctccggggc acgcgcgatc gaggccaaat cggtccagat ggaccagtcc
     2701 ggtgtcgtcg ccctctcgac tgagagcgcc gttctgctga agtcgagcgc cgtccaggtc
     2761 gtcgccgaga aggcgcgact gtccaatagc gcggcgctgt ttctgtcgag cggtgaggcg
     2821 tcgatcgaca actcacggat cgcgatcttt gccggctcgg ccgatggcga tgtccaggcg
     2881 ctgatcacgg cgaagacggc cgcgatcctg gccggtgtgc tggccgtgtt cctcgccttg
     2941 ctcgtcgcgc tgccgcgcaa tcgcacgcgg gactgactac ccgcgcagaa cacgatggag
     3001 gcgtcatgaa caacgggacg aaactcatcg tcggctcgct gcttggcgcc ggtgtcggct
     3061 acatcgtctc caaagtgatg gagcgcgaag tcgtcgagcc cgtctccttc accgacaccc
     3121 caccggaacc gaaagagacc ttcaaggaac gctggcaacg cgccaagctc gccggcgaag
     3181 aagcccgcct cgccaaggag gccgaactcc gcgcctattt ccgcacaaag gtcaacgatc
     3241 caaacgcgtt gacgacggaa tagggattcg ggaaccggga ttcgggggac gaagccgatc
     3301 cgtggaaacg cgtcacgcgt ctccctttca cggacaatcc tcgaatcccg aatcccggtt
     3361 cccgaatccc tcacttctgg catacccctt gcgttcctcc aaccggccgc atggccgtgg
     3421 agtccatgcg ggtcagattg ccatgccggt atgtctgcca ttccggccag ggccggcggg
     3481 aaaggacgcg agacatggag aacgttagtt ccgcttcaat cggcgatatg ctggtacaga
     3541 gtatcgacac catctcgccc gatgagactg tgaccgtcgc caagcgacgg atggagtcgc
     3601 aaacatcgcg ctcgctgatc gtcgtcgacg acaaccggcc ggttggtgtc gtccagtggc
     3661 gcgggctcaa ccgccgtgag ggcagcgagc tgatccggga tgtcatgcag accgagttcc
     3721 cgattctgcg ctccggcatg tcggtcagcg acgtgcggga ctatctcagc ggcgtcgatg
     3781 ttgacctgga ccacctgccg gtcgtcgatg agagcgggat gctgctcggt gaggttccgc
     3841 gcggggtcat caccaaaggc gagacagcca ccaacgccgc gaccgaagcg gtcgtcgccg
     3901 gaccggatgc cgatccgagg cgcgatagcc agccgcaggt ccggcttgag cagggcatga
     3961 aggtgatcga cgccaacggc aagagcctcg gctcgatcga tcaggtcgag ttgagcgccg
     4021 agggccagat cgcgcacttc gctgtcaagt acggcatgct cggccgcaac tcgaagcgac
     4081 tgccggctga tgtcatcagc cgggtcgagg gtaacgaggt ccacgtcaac atcggccagc
     4141 ccgagttcaa gatgctggcc gacgtcggcg aagtcgtcat ctagcatcaa tcgctggtgt
     4201 cagtcagcgg atacgcttgg taatgatagg cggctgacaa cggctgtggc gctgctgtca
     4261 gccgccttcc ggtgtgccga ggacgtcgta gctgtcgtgg ccgaggtaga cgacgtcgag
     4321 cgggacgccg ggaacaagcg tgctgacgac ggaaccggcc agcgggccga gctgatcgga
     4381 gacgatctcc ttgagctcgt cgtccagccg gaccttcgaa tggaagaccg cctcgacctg
     4441 gagccgctgg ccatcgctgg gcggcgcgcc gttgcgggtc agcgccagca tcgagccggt
     4501 ctcatcgcgg aacgtataga ttgcatactg gctcaggatc ggcagctccc gtacgctctc
     4561 ggccgtgccg gtcagcgtca cgcggcggtt gtcccagccg gtcgggtcgg cgcgcagttc
     4621 ggcgatcgtc gtcgcttcga cgcccggctc ggactcgcgg ttgacgaaaa taccgatggc
     4681 gatcaagcca atcagcagta cgcccagcac agcaacgatc gtccccggat ggatccggaa
     4741 accgcgtcgt tcatcctcga ccggcagcgg agggattgcg ttatgctggg tgcctgattc
     4801 ctgcgtgttc atggtcagcc tagcctacca gagtggcagg cgccgagaca tcggcacaac
     4861 aacgggaccg ccagattggc ggtcccgttc gtggtgaggg ggaggtaagg agatcaggat
     4921 tccatgccgc ccgacgtggc ggtcagctcg ggagtgtcgc tcatcgacga cgcctcccga
     4981 atctgaatcc tgcgtggccg tgcctccggc gccttcggga atgtcaacgt cagcaggcca
     5041 tcggcgactg tcgcgaccgt gttatcccag tcgaccttgt acggcagatt gatgctgcgt
     5101 ccaaagcgcc cgccgcgcaa ctcacgcgcg taccaggtat aaccacggga ctcctcagcg
     5161 tcatagagct gggggaactg gccgctgatg gtgacggtgt tgtcctcaac ctgaatgtcg
     5221 acgtgctcgg cgcgcaggcc aggcacgaac gcctgaacga tcaactgctc gcccttgtcg
     5281 aacacatcga ccggcaaggt cgtgatactc gtctgcgagc gcaggccgaa tgcgtcctgc
     5341 gccagccggt caagctggtg ctgaagatag gcgaaatccg cccacgggga aaaggtcatc
     5401 gtgcggctgg tcattggtgg tgcctccctt cgaaaacctc aagagccgcg aaagaccgct
     5461 acgcttgacg cctccggagc cggggtcggc tctcaggagc ttgtggcaac atcatagact
     5521 atgactcatg atgtgtcaag tctgttactc atcagagtgt atcttgttgc gctcaccatg
     5581 gagcaacgac gcagatgtca tacgagtgag gccgacggtg tttgggcagc gatgggaatc
     5641 gacaatctga cttcgagcag gagagcaact tactccgcga gagcggccag gtactccgcg
     5701 acggcgatcg tgtggcgcca gtcctcgtcg ccctcgatcg accagcaggc ggagaggacg
     5761 gcctgggcga tgccccagcc gcggacgcgg gcgcgctcca tcccgagccc ctcggcgagc
     5821 tggtcgacgc gacgggtgat gatctgacct ggatttggca gatccagcag tcccggcatc
     5881 gggttgtaga ggaacgcgcc cagatcatag cccggatcgc cgacgatccc cttcgggtcg
     5941 atgatcagcc atggttggcg ggtcgccgag aggatgttgg cgtggtgcag gtcgccgtgc
     6001 agcaacagcg cctcggccgt cgtcgccagc agccaggcga aggcgtcttc ggcctcctcg
     6061 aagagccggg cgggtagcgg cccagtgccg ccgtcgaagc gttcgcggtg gcgcgccagc
     6121 cccgccgccc agtccgcgac ggtcggaaac cgatgtccaa ccggaggcgg gcgccagagc
     6181 cgctgcatca ggtcgatcgc gaccgccgtc gcccgctcgt cgtcctcggc aacaagcgct
     6241 gccagcggca tgccgggcag gacacgctcc agcaacgacg cgttccgctc ctcatcctgg
     6301 gccagaacgg cgcagatgcc gtcgccggcg aaggcgcgca acgcctccat ctcggcagac
     6361 gcttcctcat gcgggcaacg gaccttgagg acaaccggcg tgccatcagc ccgcctcgcc
     6421 ggcgcgacgt agttgtacga gagcgcgaac ggcggctcga ccaccagccc ccactgctcg
     6481 gcacactccg cgacgatctc cggcaaacgc gccagccagc ccagcccggc gtcgccgaag
     6541 acaccagcga tccgccggtg aaactcgtcg ctgatctgaa ccggcattcc tggcacggct
     6601 gatcctgctc gcaaccgcta tccggacgat gccagcactg gcgcattccg cgcacttcac
     6661 cgttgaaggc gtacggcacg ctacccgatc gctagcaggt gttcaatcgt cggacgtagc
     6721 tttctgatca ggtgttgcag ttgttgatat ctgatgtcct gatcaggagt cagtctctgg
     6781 tgtgacgctg catcgtccag gacatcgccg tacgactcgg ttatcggcca ctcattgagg
     6841 tagtcaagcc gggcttcttg atccagcgta tcccacgcta atgccacaga ctcgacgttg
     6901 cgccactctc ccgtgaggaa gtccagcagc cagtcgatat caatctcgat cgaacgtgct
     6961 atctgagcca tctggcatgt cctggaatgc gtgcagccgt ctcggaaacc accatatgtg
     7021 ctgtgctgat cttaccagtg tcggctgaat acagaacaag ggtgaatggc agcgccgcgc
     7081 taccgagccg gtgggatggg acggtcacga gcgtctccga gagaaacgca acggttgtct
     7141 gattccagcg acggtagatc gcgattctcg cccctggaga ccgaaccgca gacgaaacgt
     7201 ccgtaagata gtcggacaac gatgtcccga tactccactg gcgatcgtga acaacgcgct
     7261 tcagcacgtg gtacagagcg gattccgccg tcggaccaat tcgaacgcca tgaaagacga
     7321 tgcgcatctg ccagggaacc gaccactgtt tcgcactgaa tgatgccgtc gctacgtgtt
     7381 cgatgatcgc atcgatgtca tct
//
LOCUS       DASUGJ010000168         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1411962, whole genome shotgun sequence.
ACCESSION   DASUGJ010000168 DASUGJ010000000
VERSION     DASUGJ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411962"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFV93_03710"
     CDS             <1..199
                     /locus_tag="VFV93_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164478.1"
                     /translation="RNAPTPNRWAGHMSVPIRATPRNVGATGGSDPVSPESRKRGNHS
                     SPASVYLPIGFEVPDAKGKHE"
     gene            196..951
                     /locus_tag="VFV93_03715"
     CDS             196..951
                     /locus_tag="VFV93_03715"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5164479.1"
                     /translation="MKLGNRVLPVAGALVAALLLMAVAIGVAGRNEPDATAAETDATR
                     TISVNGEGRVSLAPDVVMMSVGVDERDPDLNTAQSAAAEKMDAIIAALQSNGVAEADI
                     QTGNYSISAERDYEKEGQPVTGYVVSHTVTAKIRDIDNAGNVVSAAIDAGANNVGGIW
                     FGLDDPAAAIQQARELAVADAEAKATELARLANATLGPIQVISEGSTPSSPAVPFNGA
                     GAAYDEAVRSSSPTINPGQTEVVLTVYVTYAIS"
     gene            complement(1059..1781)
                     /locus_tag="VFV93_03720"
     CDS             complement(1059..1781)
                     /locus_tag="VFV93_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX5164480.1"
                     /translation="MQTPIHERATLPARVRVLAICAHPDDIESWCGGTLALLVERGCE
                     VRLAVCTAGEKGSSDRSLTPEEIAGTRIAEQRAAAARIGVSAVRFLDGKDGELEDTLN
                     FRAQIVRLLREFQPEVVFTHDPEQPYPPYTSHRDHRVVGRVVLDAAYPAARDHLYFPE
                     QIREGLPTHAVSEVWLFASSVATTAVDISTTLERKIVARLDHRSQTKDPDDLRVAWRD
                     RAANIGAPFGLAAAETFTVVRL"
     gene            complement(1827..>2844)
                     /locus_tag="VFV93_03725"
     CDS             complement(1827..>2844)
                     /locus_tag="VFV93_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5164481.1"
                     /translation="ATAEPTTTPAIPIVPSDGTTSGEPMAELVNRLNPAVVTVINKQL
                     FEGFFSAGADLLPVGSGTGFIVSDDGYIVTNNHVVEGSSAIDVIFQDGTTIEAHLVGT
                     DPFTDIAVVKIEGGVPATVPLGSTANLRPGDTVVAIGSALGSYTNTVTTGVVSGLGRR
                     LEVATGVSSENLIQHDAPINPGNSGGPLFNLSGEVIGVNTYAIHQAADGRYADGLGFA
                     ITSETVQQIMTILIAEGKVSRPYFGISYVELNPLVAAASGLPVEQGILVTNVPSGGPA
                     REVGIVEGDIITAINGQQIDREHPFVNLLYQLHAGDTIDVEVYRRTTDEALTFQLTLQ
                     ARAE"
BASE COUNT          476 a          961 c          948 g          459 t
ORIGIN      
        1 gcgcaacgcg cccacaccca accgctgggc aggacacatg agcgtaccca tccgcgccac
       61 gccgcgcaac gtcggggcga ccggcggcag cgatcccgtg tctcctgaat cccgaaagcg
      121 cgggaaccat tcgtcaccgg ccagcgtcta tcttccaata ggctttgaag tacctgatgc
      181 gaaagggaag cacgaatgaa gctcgggaac agggtcctgc ctgtcgccgg cgcgctggtg
      241 gctgcgctgc tgctgatggc cgtcgcgatc ggtgtcgccg gccgcaacga accggacgcg
      301 accgccgccg agaccgacgc gacgcggacg atcagcgtca acggcgaggg gcgcgtctcg
      361 ctggctccgg atgtcgtgat gatgtcggtc ggcgttgatg agcgcgaccc cgatctgaac
      421 acggctcagt cggcagcagc cgagaagatg gacgccatca tcgccgccct ccagtccaac
      481 ggcgtcgctg aggccgacat ccagactggc aactactcga tctccgccga gcgagactac
      541 gagaaggagg gccagccggt caccggctac gtcgtctcgc acaccgtcac ggccaagatc
      601 cgcgacatcg acaacgccgg caacgtcgtc tcagccgcga tcgacgccgg cgccaacaac
      661 gtcggtggca tctggttcgg tctcgacgac ccggccgccg cgatccagca ggcgcgcgag
      721 ctggccgtcg ccgacgccga agcgaaggcg accgagctgg cccggctggc caacgccacc
      781 ctcggcccga tccaggtcat cagcgaaggc tcgacgccat cgtccccggc cgtcccattc
      841 aacggcgctg gcgcagccta tgacgaagcg gtccgctcct cctccccaac catcaacccc
      901 ggtcaaaccg aagtcgtcct gacggtttat gtgacgtacg cgatcagcta gggcggggaa
      961 ccgggattcg ggattcgggg cttggatacg cacgtgttcc taacggagcc ggtacgtatc
     1021 cccagccccg aatcccgaat cccggttccc gcgtcccatc acaaccgcac caccgtaaat
     1081 gtctccgctg cagccaggcc gaagggggca ccgatgttgg cggcgcgatc gcgccaggcg
     1141 acgcgcaggt cgtcggggtc cttcgtctgc gagcggtggt cgaggcgggc gacgatcttg
     1201 cgctccagcg tcgttgagat gtcgacggcg gtggtcgcca cgctgctggc gaaaagccag
     1261 acctcactga cggcgtgggt cggcagcccc tcgcggatct gctcggggaa gtagaggtgg
     1321 tcccgggcag ccgggtaggc cgcgtcgagg acgacccggc caacgacgcg gtgatcgcga
     1381 tggctggtgt agggcggata cggctgctcg gggtcatgcg tgaagacgac ctccggctgg
     1441 aactcacgta gcagccggac gatctgggcg cggaagttca gggtatcttc cagctcaccg
     1501 tccttgccat ccaggaagcg gacggcgctg acgccgatcc gtgccgccgc cgcccgttgc
     1561 tcggcgatgc gggtcccggc gatctcctcc ggcgtcaggc tccggtcgct gctgcccttc
     1621 tcaccggccg tgcagaccgc cagccgcacc tcgcaacccc gctcgaccag cagcgccagc
     1681 gtcccgccac accacgactc gatgtcgtcc gggtgggcgc agatcgccag tacccgcacc
     1741 cgcgccggca aggttgcccg ttcatggatg ggagtttgca aaggtgctcc cttcgtggct
     1801 cgctggacgg cgacagtcga tccggcctac tcggcccgcg cctgaagcgt caactggaac
     1861 gtcagcgctt cgtctgtcgt ccggcggtaa acctcgacat cgatcgtgtc gccggcgtgc
     1921 aactgataga gcaggttgac gaatgggtgc tcgcggtcga tctgctggcc gttgatggcg
     1981 gtgatgatat cgccctcgac gatgccgacc tcgcgggccg gaccgcccga ggggacgttc
     2041 gtgacgagga taccctgctc gaccggcagt ccggatgccg ccgccacgag cgggttcagc
     2101 tcgacgtacg agatgccgaa gtacggccgg ctgaccttcc cctccgcgat caggatcgtc
     2161 atgatctgct ggacggtctc gctcgtgatg gcgaagccga gaccgtcggc atagcggcca
     2221 tcggcggcct ggtggatcgc gtaggtgttg acgccgatga cctcgccgct cagattgaag
     2281 agcggcccgc ccgaattgcc ggggttgatc ggcgcgtcat gctggatcag gttctccgag
     2341 ctgacgccgg tagcgacctc cagccggcgg ccgagaccgg agacgacacc ggtcgtgacg
     2401 gtgttggtgt agctccccag cgcgctgccg atcgcgacga ccgtgtcacc cggccggagg
     2461 ttcgccgtgc tgcctagcgg aaccgtcgcc gggacgccgc cctcgatctt gacaaccgcg
     2521 atatcggtga acgggtcggt cccgaccaga tgcgcctcga tcgtcgtgcc atcctggaag
     2581 atgacgtcga tcgcgctgct gccctcgacg acgtggttgt tggtgacgat atagccatcg
     2641 tcggagacga tgaagccggt cccgctaccg accggcagca gatccgcccc ggcgctgaag
     2701 aatccctcga agagctgctt gttgatgacg gtcacgacgg ccgggttgag gcgattgacc
     2761 agctccgcca tcggctcgcc ggaggtcgtg ccatctgacg gcacgatcgg gattgctggg
     2821 gtcgtggttg gctcggcggt tgcg
//
LOCUS       DASUGJ010000169         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1968845, whole genome shotgun sequence.
ACCESSION   DASUGJ010000169 DASUGJ010000000
VERSION     DASUGJ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968845"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VFV93_03730"
     CDS             <1..1354
                     /locus_tag="VFV93_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5164482.1"
                     /translation="WACNSPVLCLTGQIFDNQLGKNMGALHEIPDQLGMIGHIAKWTA
                     SVNTPPEAPAAVQEAFRQLTSGRPRPVVIEMPPGVMGEPGDVTLLDPASNGAAPELDE
                     DALDKAAKILGGAKYPIIFAGGGTTEAGEALKQLAETLQAPVIMTPAAKGVLSDRHPL
                     ALSLLGGHKIWDRADAVLAVGTRFDRPLLDWGIEGLQIVRMDIDPEEFDRVTPPAVGI
                     VADAERGLAALANKVERHNGKRDSRNEEIAAVKEQVSEELFEKGQPQASIAEVIRQEL
                     PDDGRFVGDVTQVAAFADIGFPVYEPRTFITSGFQGTLGYGYATSLGVKVGCPDVPVV
                     SINGDGGFLYTMSELATAQQHGIGVVAIVFNDGAYGNVKAIQRTRYGGREIASTLENP
                     DFVTVAKAFNIAGRRAETPEQLREALREGFASNAPMLIDYPTAPMPMVRQLSRGKVRG
                     "
     gene            complement(1649..1801)
                     /locus_tag="VFV93_03735"
     CDS             complement(1649..1801)
                     /locus_tag="VFV93_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164483.1"
                     /translation="MDETTEDHLFTAADNRNFAVRIHEDSDAYAADLRWAAIAAFYAA
                     VHYVNG"
     gene            complement(1804..2112)
                     /locus_tag="VFV93_03740"
     CDS             complement(1804..2112)
                     /locus_tag="VFV93_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164484.1"
                     /translation="MATDVTTNSQVIAREFAAAIRDEPAVEQLWLVEDPGCLQLIAIT
                     SDLDDATELRIDEAFGDLMRRHPAAGIFSRIFSPSLFEEGTDMVSLVPQRARQIPLCD
                     "
BASE COUNT          453 a          760 c          751 g          403 t
ORIGIN      
        1 ctgggcctgc aactcgccgg tcctgtgcct gacgggccag atcttcgaca accagctcgg
       61 caagaacatg ggtgcgctgc atgagatccc cgaccagctc gggatgatcg gccacatcgc
      121 caagtggacc gccagcgtca acacgccacc ggaggcgccg gccgccgtcc aggaagcctt
      181 ccggcagctc accagcggcc ggccccggcc ggtcgtgatc gagatgccgc cgggcgtgat
      241 gggcgagccg ggcgacgtga cgctgctcga cccggccagc aacggcgcag caccggagct
      301 tgacgaggac gcgctcgaca aggcggcgaa gatcctcggc ggggcgaagt acccgatcat
      361 cttcgccggc ggtgggacga ccgaggctgg tgaggcgctg aagcagctgg ccgagacgct
      421 ccaggctccg gtcatcatga cgccggctgc caagggcgtc ctttccgacc gccacccgct
      481 ggcgctctcg ctgctgggcg ggcacaagat ctgggaccgg gccgacgccg tcctggctgt
      541 cggcacccgc ttcgaccggc cgctgctcga ctggggcatc gagggcttgc agatcgtccg
      601 gatggacatc gaccctgagg agttcgaccg cgtcacgcca ccggcggttg gaatcgtcgc
      661 cgacgcggag cgcgggctgg cggcgctggc caacaaggtc gagcgccaca acggcaagcg
      721 cgactcgcgc aatgaggaga tcgcagccgt caaggagcag gtcagcgagg agttgttcga
      781 gaagggtcag ccgcaggcgt cgatcgccga ggtgatccgt caggagctgc cggacgatgg
      841 ccgtttcgtc ggcgacgtca cccaggtggc tgccttcgcc gacatcggct ttccggtcta
      901 cgagccgcgc accttcatca cgtccggctt ccaggggacg ctcggctacg gctacgcgac
      961 ctcgctgggc gtcaaggtcg gctgcccgga tgtgccggtc gtctcgatca acggcgatgg
     1021 tggcttcctc tacacgatgt cggagctggc gacggcccag cagcacggga tcggcgttgt
     1081 cgcgatcgtc ttcaacgacg gcgcttacgg caacgttaag gcgatccagc gcactcgcta
     1141 cggtggccgc gagatcgcca gcacgctcga aaaccccgac ttcgtgacgg tcgccaaggc
     1201 gttcaacatc gccggccgcc gggccgagac gcccgagcag ctgcgcgagg cactccgcga
     1261 aggcttcgcc agcaacgcgc cgatgctgat cgactacccg accgccccga tgccgatggt
     1321 ccgccagctc tcacgcggca aggtccgggg ataaggaaac aggagcacga cacgcgccgg
     1381 gttcacatgc ccggcgcgtc gttgtctacg actcagtgtc gctaagctga atttcgttga
     1441 ggacggcgac acgaaccact tcaagatctg ccagcagctt gccgagcaga ctccgtgaga
     1501 tgcgcgtccg aggcacgtac cgggcgtcgt atccagtatc tctcaggcgg cggtagttgg
     1561 cattcgtact ccgtaacgct cgcacggtcg aaacaaacgt cgaacgttcg ttgtgatccc
     1621 tgggttcgag attcagtcgt tcccatagtt agccgttgac gtagtgaact gcggcataga
     1681 aggcagcaat cgctgcccaa cgaagatcgg cggcgtaagc gtcgctatcc tcatgaatgc
     1741 ggactgcgaa gttccgattg tcggcggcgg tgaagaggtg atcctccgtc gtctcatcca
     1801 ctgctaatcg cacaacggaa tctggcgagc acgctggggc accaacgaca ccatgtccgt
     1861 accttcttcg aaaaggctcg ggctgaatat acgagaaaag atgcccgctg ctggatgccg
     1921 tctcatcaga tcaccaaaag cctcatcgat tcgaagctcc gtggcatcat ccaggtcgct
     1981 tgtgatggcg atcaattgca ggcagcctgg gtcctccacc agccacagtt gctcgaccgc
     2041 cggttcgtcg cggatcgccg ctgcgaactc gcgggcgatc acctgcgagt tggttgttac
     2101 atcagttgcc acaatgtact cctcagatgc gaacgtcctc tatcattatg caacagtcgt
     2161 cagctatcaa ccggcaacgg aactcgttca gactggacgg caatcgcaac gaaaggcgga
     2221 acttccatgg tgcaggttaa ccagcaggac gccgcgaccg cggagacgct ggcggtcatc
     2281 gatcgcttca acgccgcctt caaccggcat gacgtcgatg cggtaatggc gttgatgacc
     2341 gacgatgtca tctttgagaa cacctcg
//
LOCUS       DASUGJ010000170         5119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1968893, whole genome shotgun sequence.
ACCESSION   DASUGJ010000170 DASUGJ010000000
VERSION     DASUGJ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968893"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VFV93_03745"
     CDS             <1..991
                     /locus_tag="VFV93_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5164485.1"
                     /translation="KPYFDSVEMKGGGDAPSAARAVVVTGEADWAWNIQVEPEILQQM
                     EGEGGPGATQTTPGASAERMMVNFADPNAEVNGARSEPSTQHPLFQHKEAREAIAFAI
                     RRDVIADQLYGPGGQATSNVLNAPAKFISPNTTWEFSLDKAAELLANVPGADGYSLLY
                     QTSVNSVRQKTQEIIKQDLEELGFSVELKSVDAAVFFSSDAGNPDTYPHFYTDLEMYT
                     NGPSSPYPITWAERFRTDEIASQANQWAGSNITRYNNPEYDKLHDQARVELDPDTQVE
                     LFVAMNDMAVNDFAEIGIVWRNGVTCVANNLTGYVGTTWASDVYDIKNWRRDE"
     gene            1298..2257
                     /locus_tag="VFV93_03750"
     CDS             1298..2257
                     /locus_tag="VFV93_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164486.1"
                     /translation="MGRYIVRRLITAVPTLILISMVIFSILALAPGDPLSDLALNPSV
                     PPEVRQRIRENMGLEDPIPVQYGKWAKSLFTGDFGYSYRTRAPVIDLIKQRLPTTLYV
                     SGLAFLFSILIAVPIGVLSAVKQYSVFDNVATTIAFIGFSLPTFCTGLLFILLFTIKL
                     DWLPSIYRSTVEGEGLGLLWARTRQAIMPVTVLGLFQAATLIRFVRASMLENIHQDYV
                     RTARAKGLTERSVITRHALRNALIPVVTIVALLVPGVITGAVVTEQIFRVPGMGDLLI
                     SSIRNNDTPVVMTITFLFAILVVIFNLVADVIYGILDPRIKYS"
     gene            2306..3265
                     /locus_tag="VFV93_03755"
     CDS             2306..3265
                     /locus_tag="VFV93_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164487.1"
                     /translation="MAEASEVRSQPGTSASALELGGMRARKSRSLWSDAWRQFRKHRL
                     AVAGVAVLVFLTIATLVGPLLWTKDMALINFAESTQGPSLSHPMGTDDLGRDIFARIL
                     WGGRISLAVGVAAMAVAIVLGTMIGALAGFFGGFVDSLLMRFTDMFISLPSLPLLLLT
                     IYLFRDTLREKIGPNLGPFLLIVFLIGVTNWMSVARLVRASFLSVKEKEFIEAANCIG
                     ATRSSIMFKHIMPNVLGPVIVAATLAVGSAIITESTLSFLGLGFPPDVPTWGQLLNTS
                     VNFMEIAPHMVLFPALMIFLAVLSINYVGDGLRDALDPRKTTS"
     gene            3420..4673
                     /gene="thrC"
                     /locus_tag="VFV93_03760"
     CDS             3420..4673
                     /gene="thrC"
                     /locus_tag="VFV93_03760"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5164488.1"
                     /translation="MKLRCIECGKSYEPRDTRTACECGGLLEVELAPVSVTREKFDGR
                     LGTLDHPLRSGVWRFRELMPDFPDDVIVSKPEGNTNLYHDNRLDDFAGVEVWFKHEGE
                     NPTGSFKDRGMTVGVSHAKWVGASIVACASTGNTSAAVAAYASAAGLPSIVFIPEGKI
                     SAAKLGQTLAYGPTVIQVRGDFDAAMRMVQDATARHGIYLLNSLNPFRLEGQKSIVLE
                     ALQQLRWQMPDWIVIPGGNLGNTSALGKSLREAMAAGLIDSMPRIAVSQAAGAAPFYR
                     AHENGFASYSPVQAQTVATAIQIGNPVNYAKARRVIIEFEGTVTAVDDAAIMQAKASV
                     DGVGIGCEPASAAGLAGIRRLVSEGVIQPGERVLTYLTGHLLKDGDANAAQYLEGGAG
                     DARVIAIDPTADALDRAMERLHAAI"
     gene            4660..>5119
                     /locus_tag="VFV93_03765"
     CDS             4660..>5119
                     /locus_tag="VFV93_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164489.1"
                     /translation="MQPFSVQVAGSSANLGPGFDCLAIALSSWLRVAVTPNDTGAIRD
                     TNASDLLGGANLVIEAMVTTASRLGVVLPGCEVEITSEIPVARGLGSSGAAIVGGILA
                     ATTLAGVKISDDDAIDIGGEMEGHADNVAASLLGGVTAAMRGANGYVARRL"
BASE COUNT          903 a         1620 c         1628 g          968 t
ORIGIN      
        1 caagccgtac ttcgactcgg tcgagatgaa gggcggcggt gacgcaccgt ccgctgcccg
       61 tgctgtcgtc gtcaccggcg aggcggactg ggcctggaac atccaggtcg agccggagat
      121 ccttcagcag atggagggcg aaggcggccc tggcgcgacc cagacgacac ctggcgccag
      181 tgccgagcgg atgatggtca acttcgccga tccgaacgcg gaggtgaatg gagcgcgctc
      241 cgagcccagc acgcagcacc cgctgttcca gcacaaggag gctcgtgagg cgatcgcgtt
      301 cgcgatccgg cgcgatgtca tcgccgatca gctctacgga cccggcgggc aggcgacgtc
      361 gaacgtgctg aacgcgccgg ccaagttcat ctcgcccaac acgacctggg agttcagtct
      421 cgacaaggcg gcggagctgc tggcgaacgt tcccggcgcg gacggctact cgctgctcta
      481 ccagacctcg gtgaactccg tccggcagaa gacacaggag atcatcaagc aggatctgga
      541 ggagctgggc ttcagtgtcg aattgaagag tgtcgatgcg gccgtcttct tctcgtctga
      601 cgccggcaat ccggacacct acccgcactt ctacactgac ctggagatgt acaccaacgg
      661 gccatccagc ccgtacccga tcacctgggc agagcgattc cggaccgacg aaatcgcgtc
      721 acaggcgaac cagtgggccg gctcgaacat cacgcgctac aacaaccccg agtacgacaa
      781 gctccacgat caggcacggg tcgagctcga tccggacacg caggtcgagc tgttcgtcgc
      841 gatgaacgac atggctgtca atgacttcgc cgaaatcggc atcgtctggc gaaacggtgt
      901 aacctgtgtc gccaacaacc tgaccggcta tgtcggaacg acatgggcca gtgatgtcta
      961 cgacatcaag aactggaggc gcgacgaata acgtatgagt tagtatgatt cgccggtcgc
     1021 ccctctcagg ggaggtagcg accggcgact atccagcgac agtgcaggac atgatagaga
     1081 gcggccgggg ccagccaaaa tggttcccga tctatcaggc cagattggct agtgccgcca
     1141 gggactttag tgctatcatc ttctgcattc tcggacgcga acgtgggtgt gcagggtacg
     1201 cctatcttgc cgcgagagcc agagcgtggc gagtcactca tctggtggaa cgatcgatcg
     1261 tcggcagccg agatatggga ggtagggaaa aggcaagatg ggtcgataca ttgttcgccg
     1321 tttgatcacg gcggttccca cgttgatcct catttcgatg gtgatcttct cgattctcgc
     1381 ccttgctcct ggcgatccgt tgtcggacct ggcgctgaac ccgtcggttc cgcctgaggt
     1441 tcgccagcgc atccgcgaga acatgggtct cgaagatccc atccccgtcc agtacggcaa
     1501 gtgggccaag tcgctcttca ccggtgactt cggctactca taccgcacac gcgccccagt
     1561 catcgatctg atcaagcagc gcctgccaac cacgctctac gtcagcggcc tggcgttcct
     1621 gtttagcatc ctgatcgccg taccgatcgg cgtgctctca gcggtgaagc aatactcggt
     1681 cttcgacaac gtggcgacga cgatagcctt cattggcttc tcgttaccca cgttctgtac
     1741 cgggttgtta ttcatcctgc tcttcacaat caagctcgac tggttaccct cgatctatag
     1801 atcgacggtc gaaggcgagg gactcggcct tctctgggcg cgaaccagac aggcgatcat
     1861 gcctgtaact gtgctcggtc tgttccaggc ggcgacgttg attcgcttcg tccgtgcgtc
     1921 gatgcttgag aacatccatc aggactacgt ccgaacggcg cgggccaagg gcctgaccga
     1981 gcgctcggtc atcacccgtc acgcgctgcg caacgcgctg attccggtcg tcacgatcgt
     2041 cgccctgctg gttccgggcg tcatcactgg cgcggtcgtc accgaacaga tcttccgcgt
     2101 gccgggcatg ggagacttgc tgatctcgtc catccgtaat aacgacacac cggtcgtgat
     2161 gacgatcaca ttcctgttcg cgatcctggt cgttatcttc aatctggtcg ctgatgtcat
     2221 ctacggcatc ctggacccac ggatcaagta cagctagccg cgcattcgtc ctggcatcct
     2281 tgacgcgtga agagaggaaa tccgaatggc agaagcctcc gaagttcgct cccagccagg
     2341 gacgagcgcg tccgcgctcg agctgggcgg aatgcgggcc aggaagtccc gttcgctctg
     2401 gagcgacgcc tggcgacagt tccgcaagca ccgactggcc gtcgccggtg tagcggtgct
     2461 cgtcttcctg acgattgcca cgttggtcgg tccgctgctc tggaccaagg acatggcgct
     2521 gatcaacttc gccgagtcaa cgcagggacc gtcgctctcg cacccgatgg gaaccgacga
     2581 cctcggccgt gacatcttcg cccgcatcct ctggggcggc cggatctcgc tggcagttgg
     2641 cgttgcggcc atggcggtcg cgattgtgct tggtacgatg atcggggcgc tggccggttt
     2701 cttcggcggc ttcgtcgact cgttgctgat gcgcttcacc gacatgttca tctcgctgcc
     2761 gtcgctgccg ctgctgctgc tgacgatcta cctgtttcgc gacacgctgc gcgagaagat
     2821 cggcccgaac cttggtccgt tcctgctgat cgtgttcctg atcggtgtca cgaactggat
     2881 gtcggtcgcc cggctcgtgc gcgcctcgtt cctctccgtc aaggagaagg agttcatcga
     2941 ggcggcgaac tgcattgggg cgacccgttc gtcgatcatg ttcaagcaca tcatgccgaa
     3001 cgtgctcggc ccggtcatcg ttgccgcgac gctggcggtc ggctcggcga tcatcaccga
     3061 atcgacgctc tcgttcctcg gcctcggctt cccgccggac gtcccgacct ggggccagct
     3121 actgaacacg tcggtcaact tcatggagat tgcaccgcac atggtgctct tcccggcgct
     3181 gatgatcttc ctggccgtcc tctcgatcaa ctacgttggt gacggtttgc gcgacgcgct
     3241 cgacccgcgc aagaccacca gctaacctgg tcctgatgcc ggaccgccct cgggtggtcc
     3301 ggcttttttt gtgcggtccg ccgtgacggg tgatgcgtga cgcgtgatgg gttatcgtag
     3361 ggaacattcc gcgtcacgcg tcacgcgtca cgcatcacgc atcacgacag gggtaagcga
     3421 tgaagttgcg ctgtattgag tgcggtaaaa gctatgagcc acgcgacacg cgaaccgcgt
     3481 gtgagtgcgg tggactgctg gaagtcgagc tggcgccggt cagcgtgacc cgcgagaagt
     3541 tcgatggccg gctcggcacg ctcgatcatc cgctgcgctc cggcgtctgg cgctttcggg
     3601 aactgatgcc cgacttcccc gacgacgtga tcgtctcgaa gccggagggg aacaccaatc
     3661 tctaccacga caaccggctg gatgacttcg ccggcgtcga ggtttggttc aagcacgaag
     3721 gggagaaccc gaccggctcg ttcaaggatc gcgggatgac ggtcggagtc agccacgcca
     3781 agtgggtcgg tgcgtcgatc gtcgcctgcg cctcgaccgg caacaccagc gcggccgtcg
     3841 ctgcctacgc ctccgcggcc gggctgccgt cgatcgtctt cattcccgag gggaagatct
     3901 cggccgccaa gctgggccag acgctggcct acgggccgac cgtcatccag gtgcgcggtg
     3961 acttcgacgc cgcgatgcgg atggttcagg atgcgaccgc aaggcacggc atctatctgc
     4021 tcaactctct caatccattc cggctggagg ggcagaagtc gatcgtgttg gaggcgctcc
     4081 agcagctacg ctggcagatg cccgactgga tcgtgatccc cggtggcaac ctcggcaaca
     4141 cgagtgcact gggcaaatcg ctgcgcgagg ccatggcagc cggcctgatc gacagcatgc
     4201 cacggatcgc cgtctcccag gcagccggcg cggcgccgtt ctaccgcgcg catgagaacg
     4261 gcttcgcgag ctactccccg gtccaggctc agacggtcgc gacggccatc cagatcggca
     4321 acccggtcaa ctatgccaag gcgcggcgag tgatcatcga attcgagggc actgtcacgg
     4381 ccgtcgatga cgcggcgatc atgcaggcca aggcgagcgt tgacggcgtc gggatcggtt
     4441 gtgaaccggc ctcggcggcc gggctggccg ggattcggcg gctggtctcc gagggcgtca
     4501 tccagccggg cgagcgtgtc ctgacctacc tgaccggcca tctgctcaag gacggcgacg
     4561 cgaacgctgc ccagtatctg gaaggcggag cgggagatgc gcgcgtgatc gcgatcgacc
     4621 cgaccgccga tgcactcgat cgtgcgatgg agcgtctgca tgcagccatt tagcgtccag
     4681 gtagccggat cgagcgcgaa cctcggcccc ggcttcgact gcctcgcgat cgcgctgtcg
     4741 agctggctgc gggtggccgt cacgccgaac gacaccggcg cgatccgtga cacgaacgcg
     4801 tccgacctgc tcggcggcgc gaacctcgtc atcgaggcga tggtgacgac ggcttcgcgg
     4861 ctgggcgtcg ttctgcctgg ttgcgaggtc gagatcacca gtgagatacc ggtcgcgcgc
     4921 ggcctgggct cctccggagc ggctatcgtc ggcggcatcc tggccgcgac gacgctggcc
     4981 ggtgtcaaga tctccgacga cgacgcgatc gacatcggtg gcgagatgga gggacacgcc
     5041 gacaacgtgg ctgcctcgct gctgggcggc gtcacggccg cgatgcgtgg cgccaatggc
     5101 tacgtcgcgc ggcggctgg
//
LOCUS       DASUGJ010000171         3500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_162093, whole genome shotgun sequence.
ACCESSION   DASUGJ010000171 DASUGJ010000000
VERSION     DASUGJ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162093"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFV93_03770"
     CDS             <1..371
                     /locus_tag="VFV93_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5164490.1"
                     /translation="TQPTQLRQVISPESSATMRDMLESVVHYDGSLYSKVPGYRIGAK
                     TGTAQVPSPDGGYIEDATIASIVGFGPVADPRFTVLVKIDQPQKGETGLAVSSPALTK
                     VFEQLFMLYGIPPTEPEEVP"
     gene            368..1756
                     /gene="murF"
                     /locus_tag="VFV93_03775"
     CDS             368..1756
                     /gene="murF"
                     /locus_tag="VFV93_03775"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX5164491.1"
                     /translation="MIRAHDVLTGTGGRLAGDLSRNELFRRVVHDSRDVQPDDLFVAI
                     QGENQDGHRFVPDAYENGATAVLVSEEWYSRYNLGELPAIVVPDTLVALQELATYWRG
                     LFTPHVIGITGSIGKSSTKEVIAAIAGQRFRVTRSAKSFNNEIGLPLSVLEITPDTEV
                     VVLEMGGAYRFGEISELAEIARPTIGVVTNVSHSHLGRMGSLEAIAQTKTELVESLPE
                     SGVAVLNVDDERVRAMAERAKCAVVFYGLDDSADVRATDLESHGLEGISFTLHHRMRR
                     DRVSVPLLGRHSVHMALVGFAVGFQLGLAFEEIVAGFQQPNIQLRLLLVPAINGATLL
                     DDTYNANPASSLAALNLLDELDARRKVAVFGDMLELGEFEREGHRMVGDRASQVVDAL
                     YVMGDRARIIGEQAQQARPSLSVTYLNDKPGLTAALREGLRDGDLVLIKGSRSIQLET
                     VVADLRNERNGE"
     gene            1762..2835
                     /gene="mraY"
                     /locus_tag="VFV93_03780"
     CDS             1762..2835
                     /gene="mraY"
                     /locus_tag="VFV93_03780"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX5164492.1"
                     /translation="MLHLWLPVLNIAGDIRHWFENSSVLPLDPLDNLSISLALSGVAF
                     LITLIIGKPYIEWLKAHGIGKQIRVDGPEGHFSKMGTPTMGGILFTSVVVVLTLIFNV
                     VGREAMLLPLFVLIGCSILGAIDDKLNLVGGTTSGLSEQYKMLPLVLLSLVAALVLYY
                     PLDLQRIYIPFVGQYDIGLLYIPIATVVIAGSANAVNFSDGLDTLSGGTAAVAFTAYG
                     IIAFLQGQVHVVTFCFMMVGALLGFLWYNAHPAQVFMGDTGSLALGGSLATAAFMTGQ
                     WLLLVVVGAVFCAEAGSVILQRYWFKYTRIRYGEGRRIFRIAPLHHHFEAVGWSETQI
                     TMRFWLAGMMSGLLGVALALAPV"
     gene            2832..>3500
                     /locus_tag="VFV93_03785"
     CDS             2832..>3500
                     /locus_tag="VFV93_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX5164493.1"
                     /translation="MSDGTRDVGRGTWIESDLRGQRVLLMGLGNRQGGLGVARYLVDA
                     GADVRVTDLREGDALSATLDDLAGLPIAYTLGRHEEDDFRWAEVVVRNPAVPRESKWL
                     ALARSLGKPIEMEMTLFFRACPAPILGVTGTKGKTTTTTLLAAMLRERWPDAVLAGNM
                     AVSALGQLPSIRPDTPVALELSSFQLEALDEQCLSPHVAVITNISPDHLDRYESFDDY
                     AWVKG"
BASE COUNT          550 a         1169 c         1150 g          631 t
ORIGIN      
        1 ccacccagcc gacgcaactg cgccaggtca tctcgcccga atcgtcggcg acgatgcgtg
       61 acatgctgga gtcggtcgtc cactacgacg gcagcctcta ctcgaaggtg cccggctacc
      121 ggatcggagc caagactggc acggctcagg tgccgtcgcc ggacggcggc tacatcgagg
      181 acgcgacgat cgcctcgatc gtcggcttcg ggccggttgc cgacccgcgc ttcaccgtcc
      241 tcgtcaagat cgaccagccg cagaagggcg aaaccggcct ggccgtcagc agtccggccc
      301 tgacgaaggt gtttgagcag ctcttcatgc tgtacggtat tcctccgaca gaaccggagg
      361 aggtgccatg attcgcgcac atgatgttct gaccggcacc ggcggtcggc ttgccggcga
      421 cctttcccgc aacgagctgt tccggcgggt cgtccatgac tcgcgcgatg tccagccgga
      481 cgatctgttc gtcgccatcc agggtgagaa ccaggacggg catcgattcg tccccgacgc
      541 ctacgagaac ggcgcgacgg ctgtcctcgt cagcgaggag tggtacagcc gctacaacct
      601 gggcgagttg ccggccatcg tcgtccccga cacgctggtc gccctccagg agctggcgac
      661 ctactggcgc gggctcttta cgccgcacgt gatcgggatc accggcagca tcggcaaatc
      721 cagcacgaag gaagtgatcg cggcaatcgc cggccagcgc ttccgcgtca cccgcagcgc
      781 caagagcttc aacaacgaga tcggcctgcc gctctcggtc ctggagatca cgcccgacac
      841 cgaggtcgtc gtccttgaga tgggcggcgc ctaccgcttc ggcgagatca gcgagctggc
      901 cgagatcgcg cggccgacga tcggcgtcgt gaccaacgtc tcccactcgc acctgggccg
      961 gatgggctcg ctggaagcga tcgcccagac caagaccgag ctggtcgagt cgctgccgga
     1021 gagcggcgtc gccgtcctga acgtcgatga cgagcgggtt cgagcgatgg ccgagcgggc
     1081 caagtgcgcc gtcgtgttct acggcctcga cgactcggcc gatgtccgcg cgaccgacct
     1141 ggagagtcac gggctggagg ggatcagctt cacgctgcac catcggatgc ggcgcgaccg
     1201 cgtcagtgtc cccctgctgg ggcggcacag cgtccacatg gcgctggtcg gcttcgcggt
     1261 cggcttccag ctcgggctgg ctttcgagga gatcgtggcc ggcttccagc agccgaacat
     1321 ccagctgcgg ctgttgctcg tcccggccat caacggcgca acgctgctcg acgatacgta
     1381 taacgccaac ccggcctcca gtctggctgc cctcaacctg ctggatgagc tcgacgcgcg
     1441 ccgcaaggtg gccgtcttcg gcgacatgct ggagctgggc gagttcgagc gcgagggcca
     1501 ccggatggtc ggcgaccgcg cctcgcaggt ggtcgacgcg ctctacgtca tgggcgaccg
     1561 ggcgcgcatc atcggcgagc aggcccagca ggcgcgtccg tcgctgtcgg tgacgtacct
     1621 gaacgacaag ccgggcctga cggctgccct gcgtgagggg ctgcgcgatg gcgacctggt
     1681 cctgatcaaa ggctctcgca gcatccagct ggagaccgtc gtggccgacc tgcgcaacga
     1741 gcggaatggg gagtagccgg cttgttgcac ctgtggcttc ctgtcctcaa catcgccggt
     1801 gacattcgcc actggttcga gaactcgtcg gtcctgccgc tcgacccgct cgacaacctc
     1861 tcgatctcgc tggcactctc cggcgtcgcc ttcctgatca cactcatcat cggcaagccc
     1921 tacatcgagt ggctcaaggc acacggcatc ggcaagcaga tccgggttga cggcccggaa
     1981 ggacacttct ccaagatggg gacaccgacg atgggcggca ttctattcac gtcggtcgtc
     2041 gtggtgctga cgctgatctt caacgtcgtc gggcgcgaag cgatgctgct gccgctgttt
     2101 gtcctcatcg gatgctcgat cctcggcgcg atcgacgaca agctcaatct cgtcggcggg
     2161 acgacatccg ggttgagcga gcagtacaag atgctgccgc tggtgctctt atcgcttgtc
     2221 gcggcgctgg tgctttacta ccctctggat ctacagcgca tctacatccc attcgtgggc
     2281 cagtatgaca tcgggctgct ctacatcccg attgcgaccg tcgtcatcgc cggctcggcc
     2341 aacgccgtca acttctccga cgggcttgac acgctgtcgg gcgggacggc cgccgtcgcc
     2401 ttcacggcct acgggatcat cgccttcctg caggggcagg tccacgtcgt caccttctgc
     2461 ttcatgatgg tcggcgcgct gctcggtttc ctctggtaca acgcccaccc ggcccaggtc
     2521 ttcatgggtg acaccgggtc gctcgcgctc ggcgggtcgc tcgcgacagc tgccttcatg
     2581 accggccagt ggctgctgct ggtcgtcgtc ggcgctgtct tctgtgccga ggccggatcg
     2641 gtcatcctcc agcgctactg gttcaagtac acccgcattc gctacggcga ggggcggcgc
     2701 atctttcgca ttgcgccgct gcatcatcac ttcgaggcgg tcggctggtc ggagactcag
     2761 atcacgatgc gcttctggct ggcaggcatg atgagcggcc tgctgggtgt cgcgcttgcg
     2821 ctggcgccgg tatgagcgac gggacgcggg acgtgggacg cgggacgtgg attgagagtg
     2881 acttgcgtgg ccagcgcgtc ctcttgatgg ggcttggcaa ccggcagggt ggcttgggtg
     2941 tggcccgcta cctggttgat gccggagccg atgttcgtgt caccgacctg cgcgaaggag
     3001 atgcgctgag cgcgacgctg gatgacctgg cggggctgcc gattgcctac acgctcggac
     3061 ggcatgaaga ggacgatttc cgctgggccg aggtcgtcgt ccgcaatccg gcggtcccgc
     3121 gcgaatcgaa atggctggcg ctggcgcgga gcctgggcaa gccgatcgag atggagatga
     3181 cgctcttctt ccgcgcctgc ccggccccga tcctcggcgt gaccggcacg aagggcaaga
     3241 cgacgacgac gacgctgctg gccgcgatgc tgcgcgagcg ctggccggat gccgtcctgg
     3301 ccggcaacat ggccgtctcg gcgctcggtc agctcccctc gatccggccc gacacgccgg
     3361 tcgcgctgga actgtcgtcg ttccagctcg aagccctcga cgagcagtgc ttgtcaccgc
     3421 acgtcgccgt catcaccaac atctcgcccg accacctcga ccgctacgag tcgttcgacg
     3481 actacgcctg ggtcaagggc
//
LOCUS       DASUGJ010000172         5783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1413180, whole genome shotgun sequence.
ACCESSION   DASUGJ010000172 DASUGJ010000000
VERSION     DASUGJ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413180"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VFV93_03790"
     CDS             complement(<1..1011)
                     /locus_tag="VFV93_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164494.1"
                     /translation="MNVRHLGPSTLIHVAHSHWTVLLGLLAIILAPLSSVSPAVADDA
                     DEPFVIASIQGVDEGGFVLGTDGRYVVWDAYSRPFPDGPLARAIYAADRDDGAVTLLT
                     PDSWTFDSFQLPSVDAGIVIWGQDTGTTDSGGNPLIAVIASDLATGKQVEVARNALCC
                     AAVQPDGRVTWYANDGIWMRDPRSGAPATLVWPKTESSTQFIGLLGDWAVALQVIETE
                     GKYSYYEHVQLVARQITTGESAVLDDGWKWYDTQSTAGIAGTSIAYPRLYPSPADTDR
                     AGDWLAVKKLDQAAPVYFQRPAACFYLNTRIVGDDHFLLLKCRTEQAIGGADLWVTDL
                     VRS"
     gene            complement(1050..2837)
                     /locus_tag="VFV93_03795"
     CDS             complement(1050..2837)
                     /locus_tag="VFV93_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164495.1"
                     /translation="MNRSLSTSVALVLLLAGLLLPRPTVAQPGTYDQLLAYSPPPVTG
                     SWDDLGPRTDGTYVVWVEHPIDPRPPESRYSVLAWAGIELPQRREIKRSASDAEGGSG
                     FVAYLSPEVEDGTVVWIEQTGADYTGLTTDRFELHTTTISEWQEVTIATAEPGTKICN
                     PGVFGSTVVWVEVGEQTTRVVTRDRKAEQPLTVVLETATIPELHGCYPYAIDDDQLYM
                     IAASDLGTLELMAVPPGGDQEAIPVADASEAIIARGSLTVNDATAYYRVVNDPDDIAS
                     PTVRVRSVELWSGVTRDLILDLPHTSLTNPNVSEGGSTTLLANDQYLFASFNTGDPPG
                     SNCCRVRLFAHDLRHDVTFEMMRDQNLSIDVEGDALVWHSGHGFGGFNYYAGRVSWHL
                     PTTPVAGTGTGDGWFPETHHSMLEGFLAYWRQQGGLQVFGYPLTEGYLERNADSQQWL
                     TTQFTERQRFESHPTLAGTPYAIQLGRLGAELLASQGRNWEELPAGDPASPHYFPETG
                     HGVPDQFYAYWAGHGLDFGDPGVSFRESLALFGYPLSEPMVETNADGDTVLTQYFERA
                     VFEYHPDNPPEWQVLLRRLGAEMLAARGW"
     gene            complement(2861..3037)
                     /locus_tag="VFV93_03800"
     CDS             complement(2861..3037)
                     /locus_tag="VFV93_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164496.1"
                     /translation="MRWATFFFTIVMLLGAVPASATAPGTPEFQRNWDRTDRPVQADR
                     ISRTWMWGRKPTQA"
     gene            complement(3070..4695)
                     /locus_tag="VFV93_03805"
     CDS             complement(3070..4695)
                     /locus_tag="VFV93_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164497.1"
                     /translation="MRFRCATLVVLLAGVVALAGGQPVAAHDPSFIISNDAFYAHSLV
                     TDGEYIAWRWGNTTPSSRSWGLFVARVSDATQIFSTTVASGSHAIGDDKVVWERRFRG
                     VDENNQTIADPGIYGQNLITGEPFTVTTESTYNLAMHGSVVVWVACGTTGHNDCAIRM
                     RDLAGGDTVDVARTQRYYLGGGLHYNGYVVTWQETDSAYGERVYLMGVRQGEQPVQLL
                     SETKIGRYDLNGNLLVWVDANRNLTVRDLSTSAIRVLADVMVDQHLIATDGRYVVWGQ
                     VGDTSDAAVFAWDTATDSRFTVITYPAPDNLVLDVACANGVITWRHWGGNTGPKELRA
                     AHLADVLPTGREPDPGTTDPAWNYYPETGHYLSQGFRDYWEANGGLPVFGYPLTEEYY
                     QHGFPAQFTERQRFEWHAENIGTPYQVLLGRLGAELLVAQGRDWTTFPKADPAAPQYM
                     PETGHAIAPEFWDDWTSHGLDLGDPDVSFRESLALFGYPLSEPMLETNADGDRVLTQY
                     FERAVFEYHPDNPAEYSVLLRRLGAELLDTQGW"
     gene            complement(4738..>5783)
                     /locus_tag="VFV93_03810"
     CDS             complement(4738..>5783)
                     /locus_tag="VFV93_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX5164498.1"
                     /translation="DVLLQCFERRCLTWTPSNPDGWQVETGNVGQHYYRWRYGDWPKP
                     PGELLYIQPHPFDGQCCAEVGGDIHLLAPDSSSNANLTQSPDGESWPVWSPDGSRIAY
                     VNRNQGFDVFAMDSDGTNVTRLTLRPDPDYSPHWSPDGTHIVLTSQSNVNQMGTTFVA
                     TVNADGTGYRQLAEGGHPAWSPDGSMITFEAAGQIMVMRADGTNLGQLTAGAGFPGPG
                     WTPDGEWITFFRNDDGYQYAIRPDGSDLHRLWDFPGMIAEWSPTGGRVAVFRDNDLYV
                     MNADGSHPVNITNGYEMTIEGPVWSPDGRYLAYLGGNWGSIRIASVEHPERPHRTIAH
                     TPGPESHLDWSPD"
BASE COUNT         1054 a         1787 c         1832 g         1110 t
ORIGIN      
        1 ggagcgaacg agatcggtga cccacagatc cgctcccccg atggcctgct cggtgcggca
       61 cttcagcagc aggaagtggt cgtcaccgac aatccgggtg ttcagataga agcaggccgc
      121 tggacgctgg aagtagacag gcgctgcctg gtcgagcttc ttcacggcca gccagtcgcc
      181 agcccgatcg gtatccgctg ggcttgggta cagtcgcggg taggcgatgg atgtgccggc
      241 gattccggca gtgctctgcg tgtcgtacca cttccagccg tcgtccagga ccgcggactc
      301 accggttgtg atctgacgtg ctacgagttg aacgtgctcg taatacgagt acttcccctc
      361 tgtctcgatg acctggagcg cgacggccca gtcccctagc agaccgatga attgtgtcga
      421 tgactcggtt ttcggccaga ccagcgtcgc cggggcaccg ctgcgaggat cgcgcatcca
      481 gatgccgtca ttggcgtacc aggtgactct cccgtccggc tgtaccgcag cgcagcacag
      541 cgcgttgcgc gccacttcga cctgcttccc ggttgccaga tcgctcgcga tgaccgcgat
      601 cagcgggttc ccaccggagt cggtcgtacc ggtgtcctgg ccccagatca cgatgcctgc
      661 gtcgactgag ggcaactgga agctgtcgaa ggtccacgag tcgggtgtca atagcgtgac
      721 cgccccatcg tcacggtcgg ccgcgtagat ggcgcgtgcc agtggtccgt ctggaaatgg
      781 ccgggaatac gcatcccaca cgacatagcg gccgtccgtg ccgaggacga atccaccttc
      841 gtcgacaccc tggatggaag cgatgacgaa tggttcgtca gcgtcgtccg cgactgccgg
      901 agagacgctg gatagtgggg cgaggatgat cgccagcaga ccgagcagca cggtccagtg
      961 actgtgtgcg acatggatca gagtagaagg accgagatgt cgaacgttca tcggagcgat
     1021 cccttcgtac gcgtgtgcgt cgaacacggt taccagcccc gcgctgccag catctcggca
     1081 cccagccggc gcagcagtac ctgccactcg ggcgggttat ccgggtggta ctcgaagacg
     1141 gcccgttcga agtactgggt caggacggtg tcgccgtctg cgtttgtctc gaccatgggt
     1201 tccgacaggg gatacccgaa tagtgccagc gattcgcgga aactgacgcc ggggtcaccg
     1261 aaatcgagtc cgtggccggc ccagtaggca tagaactgat ccggtactcc atgtccggtc
     1321 tcggggaagt aatgcgggct ggccggatcg ccggccggga gttcctccca gttgcgtccc
     1381 tgtgacgcca gcaactcggc acccagcctg ccgagctgaa tcgcgtaggg cgttcccgcc
     1441 agcgtcggat gactctcgaa gcgttgccgc tcggtgaact gggtcgttag ccactgctgg
     1501 ctgtcagcgt tgcgttccag atacccctcg gtcagcgggt agccgaacac ttgcaggcca
     1561 ccttgctgcc gccagtacgc caggaatcct tccagcatgg agtgatgggt ctccgggaac
     1621 cacccgtcgc ccgtcccagt accggcgacc ggagttgtcg ggagatgcca cgagactctg
     1681 ccggcgtagt agttgaagcc accgaaccca tgtccgctat gccagaccag ggcatcgcct
     1741 tcgacgtcaa tgctgaggtt ctgatcgcgc atcatttcga acgtcacgtc atgacggagg
     1801 tcgtgcgcga acagccgaac cctgcagcag ttcgatccgg gtgggtctcc ggtgttgaag
     1861 ctggcgaaga ggtactgatc gttcgccagg agggtcgtgg acccgccctc ggacacgttc
     1921 ggattggtca ggctggtgtg tggaagatcg aggatcagat cacgggtgac gccactccag
     1981 agttcgacgc tgcggacacg aacggtcggt gatgctatat cgtccggatc attgaccaca
     2041 cggtaatacg cggttgcgtc gttgaccgtc aggctcccgc gagcgatgat cgcttccgaa
     2101 gcgtctgcaa caggaatcgc ctcctggtcg ccgccgggcg gcactgccat cagttcgagg
     2161 gttccgaggt cggacgctgc gatcatgtat aactggtcgt cgtcgatcgc gtatggatag
     2221 cagccgtgca attcggggat cgtcgcggtt tcaagcacga ccgtgagtgg ttgctcggct
     2281 ttgcgatcgc gcgtcacgac cctggtcgtc tgttctccca cttcaaccca gacaaccgtc
     2341 gatccgaaga cgccaggatt gcagatcttc gtccctggtt cggctgtcgc aatggtgact
     2401 tcctgccact cggagatcgt ggtcgtgtgc aactcgaacc ggtcggtcgt cagcccggtg
     2461 tagtcagcac cggtttgttc gatccagacg accgtcccat cctcgacctc tggcgacagg
     2521 taggcaacga acccggagcc accctcggca tctgaagcgc tgcgcttaat ctccctgcgc
     2581 tgcggaagct cgatgccggc ccatgccaga acggagtagc ggctttctgg cggcctcggg
     2641 tcgatcgggt gttcgaccca gacgacgtag gtcccgtcag tgcgcgggcc aagatcatcc
     2701 cacgatccag tcacgggtgg cggagagtac gcgagcaact ggtcatacgt ccccggctgg
     2761 gcgacggttg gcctcggcag aagcagcccc gctagcagca aaaccaatgc aactgaggtg
     2821 ctgagcgatc tgttcatcgt gacctcctca tctccgttga tcacgcctga gttggtttcc
     2881 ggccccacat ccaggtgcga ctaatgcgat ccgcctgaac cggccggtcg gtacgatccc
     2941 agttccgctg gaattcgggc gtgccgggcg ctgttgcgct ggccggcacc gcgccgagca
     3001 gcatgacgat cgtgaagaag aacgtggccc accgcatcgg agtcgctcct ttcctgggtg
     3061 ccggtgccat caccagccct gggtgtccag cagctctgcg ccgaggcgcc gcagcaacac
     3121 gctgtactca gccgggttgt ctgggtggta ttcgaagacg gcgcgctcga agtactgggt
     3181 caggaccctg tcaccatctg cattggtttc aagcatcggc tcggacagcg ggtagccgaa
     3241 gagcgccaac gactcacgga aactcacatc cggatcgcca aggtcaagac cgtggctcgt
     3301 ccagtcgtcc cagaactccg gggcaatggc gtgcccggtc tcgggcatgt actgtggcgc
     3361 ggccggatcg gccttcggga aggtcgtcca gtcacggccc tgcgccacca gcagttcggc
     3421 gccgagccgg ccgaggagca cctgatatgg ggtgccgatg ttctctgcgt gccactcgaa
     3481 gcgttggcgt tcggtgaact gcgccgggaa gccgtgctgg taatactcct ccgtcagcgg
     3541 atagccgaag accggcagtc cgccgttcgc ctcccagtaa tcacggaagc cctgactgag
     3601 gtagtgaccg gtctcgggat agtagttcca ggccgggtcg gtcgtgccgg ggtcaggctc
     3661 gcgaccggtc ggcaggacat ccgcgaggtg cgccgcacgg agttccttgg gtcccgtgtt
     3721 gccgccccag tgacgccagg tgatcacccc attggcacac gcgacgtcga ggacgaggtt
     3781 atcgggagcg ggataggtga taaccgtgaa tcggctgtcc gtcgcggtgt cccaggcaaa
     3841 gactgctgca tcagaggtgt cgccaacctg gccccagacg acgtagcggc catcggttgc
     3901 gatcagatgc tggtcgacca tcacatccgc gaggacgcgg atcgccgagg tgctgaggtc
     3961 gcgaacggtg aggttccggt ttgcgtcaac ccacaccagc agatttccgt tgaggtcgta
     4021 cctcccgatc ttcgtctcgc tgagaagctg gacgggctgt tctccttgcc tgacacccat
     4081 cagatacaca cgttcgccgt atgccgaatc ggtttcctgc cacgtcacga cataaccgtt
     4141 gtagtgcagg ccacctccca ggtagtagcg ctgagtacga gcaacatcga cggtatcgcc
     4201 accggccagg tcacgcatcc ggatggcgca gtcgttgtgt ccggtcgtcc cgcacgcaac
     4261 ccagacgacc acagaaccat gcatggccag gttgtatgtg gactcggttg tcaccgtgaa
     4321 cggttcgccg gtgatgaggt tctggccgta gatgccggga tcggcgattg tctggttgtt
     4381 ttcatcgaca ccgcgaaacc gacgctccca gacgaccttg tcgtcaccga tggcgtggga
     4441 gccgctggca acggtggtgg agaagatctg ggttgcgtcc gatacccggg cgacaaacag
     4501 accccatgaa cgggaagacg gtgtcgtgtt gccccatctc catgcaatgt actcaccgtc
     4561 ggtgacgagg ctgtgcgcat agaacgcatc gttcgagatg atgaacgatg ggtcatgcgc
     4621 tgcaactggc tgcccgccag cgagtgccac gacgccggcc agcagcacga ccagcgtggc
     4681 gcatcggaac cgcatggaat ccctcacttc cccgtagatg gcatcgacaa gagccttcta
     4741 gtccggcgac cagtcgagat gcgattccgg ccccggtgta tgtgcgatcg tccgatgcgg
     4801 ccgttccgga tgctcaacgc tggcgatgcg gatcgatccc caattgccgc cgagataggc
     4861 gaggtagcgc ccgtctggcg accagaccgg gccttcgatc gtcatctcat atccattggt
     4921 gatgttgact gggtgactcc cgtcagcatt catgacgtag agatcgttgt ccctgaacac
     4981 ggcgacgcgt ccaccggtgg gtgaccattc agcgatcatc cctgggaagt cccagagtcg
     5041 gtgcaggtcg ctaccatctg gacgaatggc gtactgatag ccatcgtcat tccggaagaa
     5101 cgtgatccac tctccatcgg gcgtccatcc cggacctgga aatccggcac cggccgtgag
     5161 ctgccccagg ttggtgccgt ccgcacgcat caccatgatc tgaccggcag cttcgaacgt
     5221 gatcatcgac ccgtccggcg accacgccgg gtgaccacct tccgcgagtt ggcgatagcc
     5281 ggtcccatcc gcgttgaccg tcgcgacgaa cgttgtcccc atctggttaa cgttgctctg
     5341 cgaggtcagt acgatatggg ttccgtcagg tgaccaatgg ggcgagtagt ccggatcggg
     5401 ccgaagtgtc aaccgcgtca cgttcgtccc atcggaatcc atggcgaaga catcgaatcc
     5461 ctgattgcga ttcacgtagg cgatgcggct gccatccggc gaccagaccg gccaggattc
     5521 accatccggc gactgtgtca ggttggcgtt gcttgagcta tcaggggcca gcagatggat
     5581 gtccccgccg acttcggcgc agcactggcc gtcgaatggg tgcggctgaa tgtaaaggag
     5641 ttcacccggc ggcttgggcc agtcgccgta gcgccagcgg tagtaatgct ggccgacgtt
     5701 gccggtctcg acctgccaac cgtccgggtt cgagggtgtc caggtcaggc agcggcgttc
     5761 gaagcactga agaagcacat cct
//
LOCUS       DASUGJ010000173         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_162967, whole genome shotgun sequence.
ACCESSION   DASUGJ010000173 DASUGJ010000000
VERSION     DASUGJ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162967"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFV93_03815"
     CDS             <1..359
                     /locus_tag="VFV93_03815"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX5164499.1"
                     /translation="EYGHHIQNLQGILSRAQSNQTGPNSPGVRVELQADCFAGLWAAH
                     AAETGFLEPLTDEDIKIALDAASAVGDDRIQQKMQGQVNPETWTHGSSEQRQKWFTTG
                     YQSGDFGSCDTFSTTP"
     gene            complement(456..>2192)
                     /locus_tag="VFV93_03820"
     CDS             complement(456..>2192)
                     /locus_tag="VFV93_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5164500.1"
                     /translation="QVRDPEWAREQGLVAWVTHPLVVRGQIIGLMVMFGRQNFTNDTL
                     DELAAVADSIAQFLERKNAEGALREREDVFRRIFEAAADGLIITDLETAEVLEANPAV
                     RAMHGYSDDEFMHLKRTSMIHPDEVPIWREYRETVLAGKPARVQGRLLRKDGTSFHAA
                     VQGSPILYQGRQAVLGIIRDITHEQEQHELLEQRVEERTRELKTLLDISRTVTLTPEM
                     EPLIELIINQLRRMIDYESVAIALAEDGILTTVAVRRGEEGSQYPTSLGNRFSIESPL
                     PAWNELIRGKTVYTPNVRGDDEVAETFRWRVGDRIQTSYAHIVSFLAAPLMVKNELVG
                     VMFISSTHEDHYTQHDIDLVTATANQVAVAIQNTRLHGQTREMAALEERQRLARELHD
                     SVSQALYGIGLGAQTARTQLDRDHTKAAAPLDYVLQLAEAGMAEMRALIFELRPESLA
                     QEGLVAALEKQAAAVRARHSVPVSLSLTSEPECSLAVKETLYRIAQEAMHNTVKHAQA
                     TAIELRLAHDGSRLALDVIDNGRGFDSDASFPGHLGLVSMRERVARVHGDVSISSTPG
                     SGTRVHVDVPVA"
BASE COUNT          351 a          731 c          714 g          396 t
ORIGIN      
        1 acgagtacgg ccatcacatc cagaacctgc agggcatcct gtcccgcgcg cagagcaacc
       61 agaccggccc gaacagcccc ggcgtcaggg tcgagctaca ggccgactgc tttgccggcc
      121 tctgggctgc tcatgccgcc gaaactggct tccttgagcc gctgaccgac gaggacatca
      181 agatcgcgct cgacgccgcg tccgccgtcg gcgacgaccg catccagcag aagatgcagg
      241 gtcaggtcaa ccccgagacc tggacccacg gctcatccga acagcgccag aagtggttca
      301 caaccggcta ccagagcggc gacttcggca gctgcgacac gtttagtacg acgccatagg
      361 cgataggtgt gaggtgttag gtgttagagg tgctggtccg tgtgggtcag cgggccgtgt
      421 tcctcccgag tgaaccagcc cctaacatct atcacctacg ccaccggcac atccacatgc
      481 acccgcgtgc cactgccggg ggtgctgctg atggagacat cgccgtggac gcgggcgacc
      541 cgttcgcgca ttgagacgag gccgaggtgg ccagggaagg acgcgtcgct gtcgaagccg
      601 cggccgttgt cgatgacatc cagcgccagc cggctgccgt cgtgtgccag ccgtagctcg
      661 atcgccgtcg cctgcgcgtg cttgacggtg ttgtgcatcg cctcctgggc gatccggtag
      721 agtgtctcct tgacggccag cgagcactcc ggctcgcttg tcagtgacag ggagacggga
      781 accgagtgcc gcgcgcgcac ggccgccgcc tgcttttcga gcgccgcgac caatccctcc
      841 tgcgccagcg actcaggccg gagctcgaag atcagcgccc gcatctcggc catcccggct
      901 tcggccagct ggaggacgta gtcgagtggc gcggccgctt tggtgtggtc acggtcgagc
      961 tgcgttcggg ccgtctgggc gccgaggccg atgccgtaaa gggcttgcga gaccgaatcg
     1021 tgcagctcac gcgccagccg ctggcgctcc tccagcgccg ccatctcccg tgtttggccg
     1081 tgcagccgcg tgttctggat cgccaccgcg acctggttag cggtcgccgt cacaaggtcg
     1141 atgtcgtgct gggtgtagtg atcctcgtgc gtcgacgaga tgaacatgac gccgaccagc
     1201 tcgttcttga ccatcagcgg cgccgccagg aacgagacga tgtgggcgta ggaggtctgg
     1261 atccggtcgc cgacccgcca gcggaacgtc tccgccacct cgtcgtcgcc gcggacgttg
     1321 ggcgtatata ccgtcttgcc tcggatcagc tcgttccagg ccggcagcgg cgactcgatg
     1381 ctgaagcggt tgccgagcga ggtcgggtac tgtgacccct cctcgccacg ccggacggca
     1441 accgtcgtca ggatgccgtc ctccgccagc gcgatcgcga ccgactcata gtcgatcatg
     1501 cgccgcagtt gattgatgat cagctcgatc agcggttcca tctccggtgt cagcgtcacc
     1561 gtccgtgaga tgtcgagcag cgtcttgagc tcccgcgtgc gctcctcgac gcgctgttcg
     1621 agcagctcgt gttgctcctg ctcgtgggtg atgtcgcgga tgatgccgag gacggcctgc
     1681 cggccctgat agaggatcgg gctgccctgg acggctgcgt ggaacgaggt gccatccttg
     1741 cggagcaacc ggccctggac gcgtgccggc tttccggcga ggaccgtctc gcgatactcg
     1801 cgccagatcg gaacctcgtc cggatgaatc atggacgtgc gcttcaggtg catgaattcg
     1861 tcatcgctgt agccgtgcat cgcgcggaca gcggggttcg cttccagcac ttcggccgtt
     1921 tcgaggtcgg tgatgatgag cccgtcggcg gcggcctcga agatcctgcg gaagacatct
     1981 tcccgctcgc gcagagcccc ctcggcgttc ttgcgttcca ggaactgcgc gatcgagtcg
     2041 gcaacggccg ccagttcgtc gagcgtgtcg ttcgtgaagt tctgccgccc gaacatgacc
     2101 atcaggccga tgatctgccc acgcacgacc agtggatgtg tcacccaggc gaccagcccc
     2161 tgctccctgg cccattccgg atcgcgaacc tg
//
LOCUS       DASUGJ010000174         8588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_580276, whole genome shotgun sequence.
ACCESSION   DASUGJ010000174 DASUGJ010000000
VERSION     DASUGJ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580276"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VFV93_03825"
     CDS             complement(<1..1283)
                     /locus_tag="VFV93_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5164501.1"
                     /translation="MFKKKRRMELARPGERRRREKRESEPISRRVFLFRGGMGLAFVG
                     LGTKLWSLQIAAGNKYEEAAADNITRSETLKASRGRILDRTGVPLAENRRVWAVKIMG
                     NQLPDDDAERQRVLDTLVQSLELKNVLALDKTLIPVGSEAAVVSLCSQRLDVDGPTLL
                     AKITSQGAYLESLRDNLSDAEAEELKANLSDIPGIRVLSELNFALETHASPDLPMVVK
                     QDISREKALELAANVVYLPGVIVDDTTLIRQYAGGPAFSHLLGYVGPISEQEYDAETT
                     PTGGHIYQPDDNVGRGGVEEALEKRMRGEKGIRWIQVDSAGVERLELVDLRRDPTDGL
                     SARLTVVQQFQLAVTQALQEGIEFANSEALKAHPPKPEDADKPVVGAGVAIVINPRNG
                     EVLAMVSLPTFDNQKFIGGISQEDYNAYLNDEFTPL"
     gene            complement(1276..1818)
                     /gene="mreD"
                     /locus_tag="VFV93_03830"
     CDS             complement(1276..1818)
                     /gene="mreD"
                     /locus_tag="VFV93_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX5164502.1"
                     /translation="MIVRDQRPAVYTPHTTRPLGRILFAILLLLTGLMQATFFPATGY
                     LAIVPDFALVFLLIRSSTHGVTEGLLWATGLGLWIDTLTMDPLGMHVLALLVVALIGG
                     ATRGKLFRSGAVLPIITVLGATLVYSLVLIILSALAGEPVGGVGALRLAVMTSLLNAL
                     LVPLAYGVLLVFDRWIPRRV"
     gene            complement(1891..2637)
                     /locus_tag="VFV93_03835"
     CDS             complement(1891..2637)
                     /locus_tag="VFV93_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5164503.1"
                     /translation="MTTIAFVSDIHGNLPALQAVLADLDAHGPYDEVVGGGDFVSGGA
                     YPERVMAIVRERGWPSVRGNGDEWVIESASAGRVPARGYEQLPPPTMMQVQDWAAKAL
                     SQESLRYLLDLPLAWRTTGPSGKRLVFVHATPGSAHPIVMPDADESVLTAMLDQADTD
                     TLVYGHIHRAYLRQVGERTVACCGAVGFPLDGDPRPCYLTATDTGSGWSLEHHRVNYD
                     RDQYLSDLARSGIPNAAALIEQIRAGGASG"
     gene            complement(2766..3497)
                     /locus_tag="VFV93_03840"
     CDS             complement(2766..3497)
                     /locus_tag="VFV93_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX5164504.1"
                     /translation="MQLRIGWLYGHEMNIYGDRGNVMALARRAEWRGIPATVETIGIG
                     DRLDPTQFDIYFWGGGQDREQIAVSKDLQGEKGASLKAAIEDGVPVLAVCGGYQLLGH
                     SYHPFEGDDLPGIGVMDVTSEAGNQRYIGNVVVDAGDLGTLVGFENHSGKTWLGSGVR
                     PLGRVRVGNGNNGQDGTEGARYLNAIGCYLHGSLLPKNPQLADWLIERALERSYGSAD
                     LAPIDDTLEQQAHDGVVARAVALRQ"
     gene            3635..4246
                     /locus_tag="VFV93_03845"
     CDS             3635..4246
                     /locus_tag="VFV93_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5164505.1"
                     /translation="MKATPRRPRTRPTITEQARQQQIIAAAIETIGELGYTRATFAEI
                     AKRAGLSSTGLISYHFASKKALDQEIAARIYSRLAAHMHAAMANVDGPASALAAYIEG
                     LIAFMKVEPNALRAMAGIFMNGGIEYDADAEREATSGISDILQWGQAEGVFRAFDIAV
                     MATTIQRALDGIPLAQLTNPSLDLDTYARELVELFRLATQQRN"
     gene            4269..5015
                     /locus_tag="VFV93_03850"
     CDS             4269..5015
                     /locus_tag="VFV93_03850"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEX5164506.1"
                     /translation="MQSSTIRQRTRPEDGRRRPFLLRPGTLWFAFDLLAPTALFYVMR
                     WQGASLYLALLVPAAISAVSALISYRRDTNRQSFAPFMLALTLAAFAVALITGSDRFL
                     LAKESILTALVGFWFLHSVWAERPLTYVFTRPLLESGYGRRVGIFGPLSWEQLWERDG
                     RFRHIWRVSSVMWAVATLIDAVLRVVMAYTLPVNSVPAMQLAMFIVTGLVMQVVTNVY
                     YARSGVWQLIREGGSADETTARIAPEAHTN"
     gene            5037..5525
                     /locus_tag="VFV93_03855"
     CDS             5037..5525
                     /locus_tag="VFV93_03855"
                     /inference="COORDINATES: protein motif:HMM:NF039977.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6069 family protein"
                     /protein_id="HEX5164507.1"
                     /translation="MNATINERGRAIETTESNWQTRAIAVVAAIVVNAIILAAGRLIN
                     GDFPIATVGNDQQTIGFAQVIVVTLLVGLIAWGMLAALERTTVRARTIWTTIAVAIFA
                     ISLLGPLGSGDTTSSIVVLTCLHVGAAATIIPLMRKTAGQGGPPPFHVAHPGTPHTTD
                     TR"
     gene            complement(5431..6996)
                     /locus_tag="VFV93_03860"
     CDS             complement(5431..6996)
                     /locus_tag="VFV93_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164508.1"
                     /translation="MHATEALQLMIDRQVSRLRASLHGDRRAAILAVLRAHALQPADS
                     LASSVPDLVSGRRIANLGGNRALQLCVESIASTPTAVVADDAWSAHFLDECGRLAEAE
                     LVLRHAETGFMKLVATGAESFDAWVATKLPPASWQERVALDGWAGWLARHRQQASQPP
                     ATSIVQAMAHQLSYPPDATIGGQTIRLHCDVLERLLGIALAAPDGDIIFSASDLADRL
                     AATLPAGPASIATVLAAFTLDGENAGYHADVPGIASPPLVRLDGDTLALSRFGLTSEP
                     LLFLSRELRRRYSQDYHNSAHLRENVFRADLYELFGDKRFVTSSARIELRRESGNVRT
                     DVDAVVFDRKTGTLGIFEMKSQDPFARSTAELTRQRDNLLYANRQVSGVLAWLQRYGP
                     NELLNRVDSRTARKFRAHKVYPFVLGRYFAHPGDGPAPDRRAAWGTWPQLLRWRDSQP
                     SIATASNPIASLHAYVTKDAPVVQLPPEIGKQTIPLGAARINVYPSYAVYQGERRGTV
                     EGLPGPPSSASTG"
     gene            complement(7088..8512)
                     /locus_tag="VFV93_03865"
     CDS             complement(7088..8512)
                     /locus_tag="VFV93_03865"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEX5164509.1"
                     /translation="MTRDARLLAAMAASKALTTGLRVSRRGGGTALPGLVANRIDPRL
                     LDKLAGRLPSGAIVVAGTNGKTTTTRMLAGMLEGAGRRVVHNRSGSNLVRGISSAFAE
                     QMTLFGTGGAEIGVIEADENAFPDVVRRVQPRVILLLNLFRDQLDRYGELETIANHWR
                     GVIHDLTSDVTLVVNADDPNLAALAVETGARVVTFGLDETGYELPELPHASDAAVCRV
                     CESPLVYDALFVSHLGDYHCPNCGNRRPPLDIVAREIAVRGTESLTMVVRDERDGHEA
                     HEIQAEVALPGVYNAYNALAAVAVARVLGVGFRPILETLRTFRAAFGRLERVEYAGRS
                     LLLALAKNPVGFNEVLRMMTASPLTGPLVIGINDLDADGRDVSWLWDVDFEVLAGEQH
                     SAPIFAVGIRGHDLAVRLKYAGVPTSRLDACAAAKPLADALDHVINQTDTGDEIFLLL
                     TYTALLQLRQSLADRGAVAEFWEQ"
BASE COUNT         1466 a         2783 c         2892 g         1447 t
ORIGIN      
        1 agcggcgtga actcgtcgtt caggtaggcg ttgtaatcct cctgcgagat gccgccgatg
       61 aacttctggt tgtcgaaggt cggcaacgac accatcgcca ggacctcgcc gttgcgcgga
      121 ttgatgacga tcgcgacccc ggcgccgacg accggcttgt ctgcgtcctc cggcttcggc
      181 ggatgcgcct tgagcgcctc gctgttggcg aactcgatcc cctcctgtag cgcctgggtg
      241 acggccagtt ggaactgctg aacgaccgtc agccgggcag acaggccgtc agtcggatca
      301 cggcgcaggt cgaccagctc cagccgctcg acgccggccg aatcgacctg aatccagcgg
      361 atgcccttct cgccgcgcat ccgcttttcg agcgcttcct cgacaccgcc acgcccgacg
      421 ttgtcgtccg gctggtagat gtggccgccg gttggcgtcg tctcggcgtc gtactcctgc
      481 tcgctgatcg ggccgacgta gccgagcagg tgggagaagg ccggcccacc cgcgtactgg
      541 cggatcaggg tcgtgtcgtc gacgatcaca cccggcaggt agacgacgtt ggcggccagc
      601 tccagcgcct tctcgcggct gatgtcctgc ttgacgacca tcggcagatc gggcgaggcg
      661 tgtgtttcga gcgcgaagtt caattcgctg agcaccctga tgccaggaat gtcggacagg
      721 ttcgccttca actcctccgc ctctgcgtca ctgaggttgt cccgcagcga ttccagatag
      781 gcgccctggc tggtgatctt cgccagcagg gtcgggccat cgacgtcgag gcgctgcgag
      841 cagaggctga cgaccgccgc ctccgagccg accgggatga gcgtcttgtc gagcgcaagc
      901 acgttcttga gctccagcga ctggacgagc gtgtccagca cgcgctggcg ctcagcgtcg
      961 tcatccggca actggttacc catgatcttg acggcccaga cgcgtcggtt ctcggccagc
     1021 gggacgccgg tgcggtcgag gatccgcccc cgtgacgcct tgagcgtctc cgagcgggtg
     1081 atgttgtcgg cggcggcttc ctcgtacttg ttgccggcgg cgatctggag cgaccagagc
     1141 ttggtgccga ggccaacgaa ggccagcccc atcccaccac ggaagaggaa gacgcgccgc
     1201 gagatcggct cactctcgcg cttctcccgt cgccgtcgct cgccggggcg agccagttcc
     1261 attcgtcgct tcttcttaaa cacggcgcgg tatccaacgg tcaaacacga gcaagactcc
     1321 gtaggcaagc ggcaccagca aggcgttcag cagcgatgtc atgacggcca gccggagggc
     1381 gccaacaccg ccaaccggtt cgccggccag cgccgacagg atgatcagaa cgagactgta
     1441 gacgagcgtc gcacccagaa cagtgatgat cggcaggacc gcgccggagc gaaagagctt
     1501 gccgcgcgtc gcgccgccga tcagggccac caccagcagg gcgaggacgt gcatcccgag
     1561 cgggtccatc gtcagcgtgt cgatccagag tcccagcccg gtcgcccaga gcaggccctc
     1621 ggtgacccca tgggttgagc tgcggatcag caggaagacg agcgcgaaat cggggacgat
     1681 cgcgaggtag ccggtggccg ggaagaaggt ggcctgcatg agcccggtca gcagcagcag
     1741 gatcgcgaag aggatacggc cgagcggtcg tgttgtatgc ggcgtgtaga ctgcgggtcg
     1801 ctggtcgcgc acgatcatcg cttgcgtccc caccctcgac gatgcctccc ctgaccaacc
     1861 atccggcgat gataccacga tccaatggcg ctaccccgag gcgccgccgg cccgtatctg
     1921 ctcgatcagg gcggccgcgt tcgggatgcc ggagcgggcc aggtcggaca ggtactggtc
     1981 gcggtcgtag ttgacacgat ggtgttcgag cgaccagccg ctgccggtgt cggtcgccgt
     2041 caggtaacag ggacgcggat cgccatccag cgggaagccg accgcgccgc aacaggcaac
     2101 ggttcgctcg cctacctggc ggagataggc gcggtggatg tgcccgtaaa cgagcgtgtc
     2161 ggtgtcggcc tggtcgagca tcgcggtcaa cacgctctcg tcggcatccg gcatgacgat
     2221 cggatgggcc gaccccggcg tggcatggac gaagacgagc cgcttgccgc tcgggccggt
     2281 cgtccgccat gccagcggca gatcgagcag gtaacgcaac gactcctggc tgagtgcctt
     2341 cgcggcccag tcctgaacct gcatcatcgt cggcggcggt agctgctcgt aaccacgggc
     2401 tggcacccgg ccggcactgg ccgactcgat cacccactcg tcgccgttgc cacgcaccga
     2461 cggccagccc cgctcgcgga cgatcgccat cacccgctcc gggtacgcgc caccggagac
     2521 gaagtcgccg ccgccaacga cctcgtcata cggcccgtga gcgtcaagat cggccagaac
     2581 ggcctgtagc gccggcaggt tgccgtgaat gtcggatacg aacgcgatcg ttgtcatctt
     2641 gtctccttgg tcgggattgg ggattcggga accgggagtc ggggttcggt atttgaaggc
     2701 gtctacagga atcgcaccgt tcgacaatcc cggttcccga atccccaatc ccgaatcccg
     2761 tcacatcact gccgcagcgc aacggcccgg gcgacgacgc cgtcgtgagc ctgttgttcg
     2821 agcgtgtcgt cgatcggtgc gaggtcggcg ctgccgtagc tgcgctccag tgcacgctcg
     2881 atcagccagt cggccagctg cgggttcttc ggcagcagcg agccgtgcag gtagcagccg
     2941 atggcgttca ggtaacgtgc gccctcggtg ccgtcctgac cgttgttgcc gttgccgact
     3001 cgaacacggc ccagcggccg aacgccggag ccgagccagg tcttgccgga atggttctca
     3061 aagccgacca gcgtccccag gtcgccggca tcgacgacga cgtttccaat ataccgctgg
     3121 ttcccggcct cgctggtgac gtccatcacc ccgatgccgg gcaggtcgtc accctcgaac
     3181 ggatgatacg agtggcccag caactggtag ccgccgcaga cggcgaggac cggcacaccg
     3241 tcttcgatgg ctgccttgag gctcgcgccc ttctccccct gaagatcctt cgagacggcg
     3301 atctgctcac gatcctgccc accgccccag aagtagatgt cgaattgggt cgggtccagc
     3361 cggtcgccga tgccgatcgt ctcgaccgtc gccgggatgc cgcgccactc cgcccgccgc
     3421 gccagcgcca tcacgttacc ccggtcgccg tagatgttca tctcatggcc gtagagccag
     3481 ccgatccgaa gttgcatggg tgtcctccgt gacgcgggat gcgtgacgcg tcgttgcgaa
     3541 gtgtacaggt gcgacacgct gcgatggatg caggttggcg tccgagagtt gtgcagggcc
     3601 tggacgattt gctagtctgt gctaatattc gctcatgaaa gcaacgcccc gacgtcctcg
     3661 gacgcgcccg accattacag agcaggcacg gcagcagcag atcatcgcag cggcgatcga
     3721 gacgatcggc gagcttggct acacacgtgc gacgttcgcg gagatcgcca agcgggccgg
     3781 cctgagcagc accgggctga tctcttacca cttcgccagc aagaaggcgc ttgatcagga
     3841 gatcgccgcg agaatctaca gccgtctcgc ggcacatatg cacgcagcga tggcgaacgt
     3901 ggacggtccg gcgtcggcgc tggccgccta catcgagggg ctgatcgcct tcatgaaggt
     3961 cgagccgaat gccctgcggg cgatggccgg catcttcatg aacggtggca tcgaatatga
     4021 cgctgacgcc gagcgcgaag cgacatcggg aatcagcgac atcctgcaat ggggacaggc
     4081 cgaaggcgtc ttccgcgcct tcgacattgc ggtgatggcc accacgatcc agcgcgcgct
     4141 ggatgggatt ccactggcgc aattgaccaa tcccagcctc gatcttgata cctacgctcg
     4201 cgagctggtt gagctgttca ggctggcgac gcaacaacgc aactgaccac aacgagatga
     4261 gatttccgat gcagtcatcg acgatcaggc agcgaacgcg gccggaggac ggccgccggc
     4321 ggccattcct cttgaggcca ggcaccctct ggttcgcctt cgacctgctg gcgccgacgg
     4381 cgctgttcta cgtgatgcgc tggcaggggg ccagtctcta tctggcgctg ctggtcccgg
     4441 cggccatctc ggccgtcagc gcgctgatct cgtaccggcg cgacaccaac cgccagagct
     4501 tcgcgccgtt catgctggcg ctgacgctgg ccgcgttcgc cgtcgcgctg atcaccggta
     4561 gtgaccgctt cctgctggcg aaagagagca tcctgacggc gctggtggga ttctggttcc
     4621 tccacagtgt ctgggccgag cgcccgctga cgtacgtgtt cacgcgcccg ttgctggaga
     4681 gtggctatgg ccgtcgggtg gggatcttcg ggccgctctc ctgggagcag ctctgggagc
     4741 gggatggccg ctttcggcac atctggcgcg tgtcgagcgt gatgtgggcg gtcgcgacgt
     4801 tgatcgacgc ggtcctgcgg gtcgtgatgg cctacacact gccggtcaac tcggtgccgg
     4861 cgatgcagct ggcgatgttc atcgtcaccg gactcgtgat gcaggtcgtg accaacgtct
     4921 actacgcccg ctcaggggtc tggcaactga tcagggaagg aggctcagcc gatgaaacca
     4981 cggccaggat agcgcccgag gcgcatacga actgatatat ggaaggtatt gaccctatga
     5041 acgcaacaat caacgaacgc ggtcgagcga ttgagacgac cgaatccaat tggcaaacgc
     5101 gggctattgc agtcgttgca gcgatcgtcg tcaacgccat catcctcgcc gccgggcggc
     5161 tgatcaacgg cgatttcccg atcgcgacag tcggcaacga ccagcagacg atcggattcg
     5221 cgcaggttat cgtcgtcacg ctgctggttg ggctgatcgc ctggggaatg ctggcggctc
     5281 tggagcgaac gaccgtgcga gcacggacga tctggacgac gatcgccgtc gccatcttcg
     5341 ccatctcgct gctcggcccg ctcggcagtg gcgacacgac ctcctctatc gtcgtcctca
     5401 cctgcctgca cgtcggcgcg gcagcgacga tcatcccgtt gatgcggaag acggcgggcc
     5461 agggaggccc tccaccgttc cacgtcgctc accctggtac accgcatacg acggatacac
     5521 gttgattctc gctgcgccaa gcggaatcgt ctgcttccca atctcgggcg gtagctgaac
     5581 aaccggtgcg tctttcgtta cgtaggcgtg cagcgaggcg atcgggttcg aggcagtcgc
     5641 gatcgagggt tggctgtcgc gccagcgcag gagctggggc caggtgcccc aggcggcacg
     5701 gcggtcgggg gcgggaccgt cgccggggtg ggcgaagtag cggcccagca cgaacgggta
     5761 gaccttgtgg gcgcggaact tcctggccgt tcgcgagtcg acccggttga gcagctcatt
     5821 cggaccgtag cgctgtagcc aggcgaggac gccggagacc tgccggttcg cgtagagcag
     5881 gttgtcgcgc tggcgcgtca attcagccgt cgaacgggcg aacgggtcct gcgacttcat
     5941 ctcgaagata ccgagtgtgc ctgtcttgcg gtcgaagacg acggcgtcga cgtcggtccg
     6001 gacgttgcca ctctcgcggc gtagctcgat ccgggcgctg ctggtgacga agcgcttgtc
     6061 accgaacaac tcgtagagat cggcgcggaa gacgttctct cgcaggtgcg cgctgttgtg
     6121 ataatcctga ctgtagcggc gacgtagctc gcgcgacagg aacagcaacg gctcgctggt
     6181 cagcccaaac cgcgagaggg caagcgtgtc gccatcgagc cgcaccagcg gcggcgacgc
     6241 gatgccgggc acgtcagcgt gataacccgc gttttcacca tccagcgtga atgcagccag
     6301 gacagtcgcg atcgaagccg ggccggccgg taatgtcgcg gcgagccggt cggccaaatc
     6361 gctggcggag aagatgatgt ccccgtccgg cgcggctagt gcgataccca gcagccgttc
     6421 gagtacatca cagtggagcc ggatcgtctg accgccgatc gtcgcatccg gcgggtagct
     6481 gagctggtgc gccatcgcct ggacgatgct ggtcgccggt ggctggctcg cctgctgccg
     6541 gtggcgggcc agccaaccgg cccaaccgtc gagtgcgacc cgctcctgcc agctcgcagg
     6601 cggcagcttc gtcgcgaccc aggcgtcgaa gctttcagca cctgtggcga cgagcttcat
     6661 gaagccagtc tcggcatggc gcaacaccag ttctgcttcg gcaaggcggc cgcactcgtc
     6721 gaggaagtgt gcggaccagg cgtcatcagc gacgacggcg gtcggtgtgc tggcgatcga
     6781 ttcgacgcac agttgcagag ctctgttacc gccaaggttg gcgattcgac gaccggagac
     6841 gaggtcggga actgacgacg ccaacgaatc ggccggctgt aacgcatgcg cgcggaggac
     6901 ggccaggatc gcggcccgcc gatccccgtg caggctggcc cgcaagcggc tcacctgccg
     6961 gtcgatcatc aattgcaagg cttcggtcgc atgcatttgg tggtgtcatt tccgtaggac
     7021 aaacgacggt gcctgacagg gagtgaaccc gcaagcccgc gtcgttgtca acggcgtgcg
     7081 actgagacta ctgctcccag aactcggcaa cggcgccacg gtcggcgagc gattggcgga
     7141 gctggagcag agcggtgtac gtcagcagca ggaagatctc gtcgccggtg tcggtctggt
     7201 tgatgacgtg gtcgagcgcg tcagccagcg gtttggcggc ggcgcaggcg tcaagtcgtg
     7261 aagtcgggac accggcgtac ttgagccgga cggccaggtc gtgcccgcga atgccgaccg
     7321 cgaagatcgg ggcgctgtgc tgctcgccgg ccagcacctc gaagtcgacg tcccagagcc
     7381 aggagacatc gcggccgtcg gcgtcgaggt cgttgatgcc gatcaccagc gggccggtca
     7441 gcgggctggc cgtcatcatc cgcagcacct cgttgaagcc gaccgggttc ttggccagcg
     7501 ccagcagcag cgagcggccg gcgtactcga cacgttccag ccggccgaag gcagcccgga
     7561 aggttcgcag cgtctccaga atcggccgga acccaacacc gaggacgcgc gccaccgcaa
     7621 cggccgccag cgcgttgtag gcgttgtaga cccccggcaa cgcgacctcg gcctggatct
     7681 cgtgggcctc gtgaccgtcg cgttcgtcgc ggacgaccat cgtcaggctc tctgtccctc
     7741 gaaccgcaat ctcgcgggcg acaatgtcca gcggcgggcg acggttgccg cagtttgggc
     7801 aatggtagtc gccgagatgg gagacgaaga gcgcgtcata gacgagcggc gactcgcaga
     7861 cccggcagac ggcggcgtcg gaggcatgcg gcagttcggg caattcgtag ccagtctcgt
     7921 ccagcccgaa cgtgaccaca cgcgcgccgg tctcaaccgc cagcgcggcc aggttcggat
     7981 cgtcggcatt gacgaccagc gtcacgtcgc tggtgaggtc gtggatgacg ccgcgccagt
     8041 ggttggcgat cgtctccagc tcgccgtagc ggtcgagctg gtcgcggaac aggttgagca
     8101 gcaggatgac gcgcggctgg acgcggcgga cgacgtccgg aaaggcgttc tcgtcggcct
     8161 cgatgacgcc gatctcggcg ccgccggtgc cgaagagcgt catctgttcg gcgaaggccg
     8221 atgagatgcc acggaccagg ttcgagccgg agcggttgtg gacgactcgc cgaccggctc
     8281 cttcgagcat cccggccagc atccgcgtcg tcgtcgtctt gccgttagtg ccggccacga
     8341 cgatcgcgcc ggatgggagc cggccggcca gtttgtcgag caggcgcggg tcgatccggt
     8401 tggcgaccag ccccggcagc gccgtccctc caccccggcg ggagacgcgc aggccggtcg
     8461 tcagcgcctt gctggcggcc atcgcggcca gcaggcgcgc atcgcgcgtc atggcgtgcc
     8521 cgtctcgccg ctctcgccgg tgatgaccgt cactgatgag agctcggcga agtcaaccag
     8581 cggcagga
//
LOCUS       DASUGJ010000175         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_302098, whole genome shotgun sequence.
ACCESSION   DASUGJ010000175 DASUGJ010000000
VERSION     DASUGJ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302098"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFV93_03870"
     CDS             <1..756
                     /locus_tag="VFV93_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX5164510.1"
                     /translation="LGYLARRVLGQYDLALLGREPIETSGKLYFVQPNIRNTERTLGI
                     PSDQFRLWLALHETTHAFEFEAYPWVREHMNAMLQEYFTFLTEDIEYLKRGVSGLKAF
                     WERARNNNTGDGAAWIELVMSPEQRRLFGQMQATMAVIEGYSNYIMNAVGRDLMPDYT
                     QIARRFEQRQQQKSQAEQFFIKLTGLDVKMEQYRLGESFINEVVRLRGDRFAHRVWEG
                     PELLPTMAELRQPASWIARVESRNVLDAGAASG"
     gene            753..1940
                     /locus_tag="VFV93_03875"
     CDS             753..1940
                     /locus_tag="VFV93_03875"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEX5164511.1"
                     /translation="MTLDLSRVITSISEMVATVDPDHERHRYDALRHLWETLNPDDLD
                     ARLDQAGTTFLTILQTGEMQRRYPLPALPANYAVAATDGSMMTPDRHSPARFYLINVG
                     QVYLRYGDNPTANLTSEPDLRFAEDDLWVNASNRRVPINETILGLRRAVAELRAAVNL
                     LEGEPLPAIAFVDGTLILWMLESLDDEIISTLLDEFMEVLDVLKAYRIPVAGYISSPG
                     ATDMFGTMRVAACDYPTHGRAIDCIDCWRRIINEGHTAACDILPRVTDRYIVEAVAQL
                     QPGERTAAFPSESRILRRYTPQQRVWFCYLHTGTEIARIEIPAWVATDRELLDRVHAI
                     VWDQAQLGRGYPVALQEAHEQAVLSMADRRLVEQTVERQLASQGIIVTRTGKDGSKRG
                     RFV"
     gene            1924..>2541
                     /locus_tag="VFV93_03880"
     CDS             1924..>2541
                     /locus_tag="VFV93_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164512.1"
                     /translation="MAALSESLATARLGEVIETNTTGFAAESDQLHRLPELGALVCVG
                     GLEETRSYYGVVSFGETGGLDTSRKAVRRGGDHLQDEAIYDRHPELDMVLRTIFEVAV
                     IGYGDGPGYRHTLPALPVPLHYSVHGCDRTTTAAFTANPRDLASLLSFHGEIMPEQLL
                     AAHLRWVDRQLNDDQAWLADATRRLARLMKRDYDRLSMILEAVAPD"
BASE COUNT          473 a          894 c          752 g          422 t
ORIGIN      
        1 ctcggatacc tcgcccgccg cgtcctcggc cagtacgacc tcgcgctgct gggccgcgag
       61 ccgattgaga ccagcggcaa gctctacttc gtccagccca acatccgcaa caccgagcgc
      121 acgctaggca tcccgtccga ccagttccgg ctctggctcg cgctgcacga gacgacccac
      181 gccttcgagt tcgaggccta cccgtgggtc cgcgagcaca tgaacgcgat gctgcaggaa
      241 tatttcacct tcctgacgga agacatcgaa tacctcaagc gtggcgtcag cggcctgaag
      301 gcgttctggg agcgtgctcg caacaacaac accggcgacg gcgcggcctg gatcgaactg
      361 gtaatgtccc cggaacaacg ccgcctgttc ggccagatgc aagccacgat ggccgtgatc
      421 gagggctatt cgaactacat catgaacgcc gtcggccgtg acctgatgcc ggactacacc
      481 cagatcgcgc gccgcttcga gcaacgccag cagcagaagt cgcaggccga gcagttcttc
      541 atcaagctga ccgggctgga tgtcaagatg gaacagtacc ggctcggcga gtcgttcatc
      601 aacgaggtcg ttcgcctgcg gggcgaccgc ttcgcacatc gcgtctggga ggggccggaa
      661 ctgctgccga cgatggctga gttgcgccaa cccgccagtt ggatcgcgcg ggtcgagagt
      721 cgcaacgtgc ttgacgctgg agcagcatcc gggtgacgct cgacctctcg cgcgtcatca
      781 ccagcatctc ggagatggtc gcgaccgtcg atcccgacca tgagcgccat cgatatgacg
      841 cgttgcgcca cctctgggag acgctcaatc ccgacgacct ggatgcccgc ctcgatcagg
      901 caggcacgac cttcctgacg atcctccaga ctggcgagat gcaacgccgc tacccgctgc
      961 ccgctttgcc ggccaattac gccgtcgccg ccaccgacgg gtcgatgatg acaccggacc
     1021 gtcatagtcc ggcccgcttc tacctgatca acgtcgggca ggtctacctg cgctatggcg
     1081 acaacccgac cgcgaacctc accagcgaac cggatctgcg cttcgccgag gacgatctct
     1141 gggtgaatgc cagcaaccgc cgcgtgccga tcaacgagac gatactcggt ctgcggcggg
     1201 cggtcgccga gttgcgagcc gccgtcaacc tgctcgaagg cgagccgctt ccggccatcg
     1261 ccttcgttga cggaaccctg atcctctgga tgcttgagtc actcgacgac gagatcatct
     1321 cgacgctcct cgacgagttc atggaagtcc tggatgtcct gaaagcgtac cgaatcccgg
     1381 tagcaggcta tatctcgtcg cccggcgcga ccgatatgtt cggcacgatg cgcgtcgcgg
     1441 cctgcgacta cccaacacat ggtcgcgcca tcgactgcat agattgctgg cggcgcatca
     1501 tcaacgaggg tcacaccgcc gcctgcgaca ttcttccgcg cgtgaccgat cgctacatcg
     1561 tcgaagccgt cgcccagctc cagcctgggg agcggacggc cgcgttccca agcgagtcac
     1621 gcatcctgcg acgctacacg ccgcagcaac gcgtctggtt ctgctatctc cacaccggca
     1681 ccgagatcgc ccggatcgag attcccgcct gggtggcaac tgaccgcgaa ctgctcgacc
     1741 gcgtccacgc catcgtctgg gatcaggcgc agcttggtcg tggctacccc gtcgcgctcc
     1801 aggaagcgca cgagcaagcg gtcctcagca tggcggaccg gcgactggtc gagcagaccg
     1861 tcgaacggca gcttgccagt cagggcatca tcgtcacccg aactggaaag gacggcagca
     1921 agcgtggccg ctttgtctga gtcactcgcc accgcgcgcc tcggcgaagt catcgagacc
     1981 aacacaaccg gtttcgccgc cgagagcgat caactgcatc gcctgccgga actcggcgcg
     2041 ctcgtctgtg tcggcgggct ggaagagact cgctcgtact acggcgtcgt ctcctttggc
     2101 gaaactggag gactggacac cagccgcaag gcagtccgac gcggtggcga tcacctgcag
     2161 gacgaagcga tctatgaccg ccatcctgag ctcgacatgg tgcttcgcac gatcttcgag
     2221 gttgccgtca tcggctacgg cgatggtccc ggctaccgcc acaccttgcc ggccctgccg
     2281 gtgccgctcc actacagcgt ccacggctgc gaccggacga cgacggctgc cttcaccgcc
     2341 aacccgcgcg acctggcgtc gctgctcagt ttccacggcg agatcatgcc cgaacagctg
     2401 ctggcggccc acctgcgctg ggtcgatcgc cagctcaacg acgaccaagc ctggctcgcc
     2461 gacgccaccc gccgccttgc ccgcctcatg aaacgcgact acgaccgcct cagcatgatc
     2521 ctcgaagcag tggctccgga c
//
LOCUS       DASUGJ010000176         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_580464, whole genome shotgun sequence.
ACCESSION   DASUGJ010000176 DASUGJ010000000
VERSION     DASUGJ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580464"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFV93_03885"
     CDS             <1..210
                     /locus_tag="VFV93_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164513.1"
                     /translation="IGTVDAWRVEVTFEANDAFKATSQTGGIIDRSFNLLHEGESWYW
                     FPATGELDSFKEGSCGLPWPGHSPV"
     gene            complement(266..2026)
                     /locus_tag="VFV93_03890"
     CDS             complement(266..2026)
                     /locus_tag="VFV93_03890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5164514.1"
                     /translation="MRRPTVRVDRRSEVLGWAAFTFLLSCALLAFVLPDLGRYPTVSG
                     DDSWVMSASYKLATEGVFGSDLYAGFHGADQHYFVALPVYQLLQALSFSLFGAGIVTA
                     RLVTVASALVVLWATTWLALRWYGALAAGICGLLLAGLRVNLVDLWPGLPLLAVGRTG
                     RYDMTGVAFTMLTVVCLDRAIERGPDRQAWTPWLTGICAGLATLTQFFGAFTVVIIAL
                     TLGWHGRQRVSLGWRIAVGWTAVVAPYLLYVAVHWADFHGQAALKAGRTGFLDPTFYL
                     DNLLHEGDRYRHLIETGLTAPSGALNWLFVLGIVPLIAWLGWRARQPERTGDRLLLTL
                     LLGGAGSLALLDSTKAPLYVIVLWPGCCIAIAAAGAAGIAWARGRFTQDQPAAERWLL
                     VAGCCVALVVAAVIVADGADAYRDDRLRAQAVSNYENVAQQLDAAIPAGTGIIGHERW
                     WWGLHERDYRALNVLQLTWEETSGTPSFAAQFDATGAGALIIDDNARSEIGRYPAALQ
                     AQIAVFLAERAAPPVIISDPTYGRFEIYILRRRQTAGEVARPVRLWKVVTEDGPEEAR
                     RTRGYARGCDVYSRMQATLV"
     gene            complement(2107..>2542)
                     /locus_tag="VFV93_03895"
     CDS             complement(2107..>2542)
                     /locus_tag="VFV93_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164515.1"
                     /translation="ANHNFRHVGQDEAERAGEHRLLALMPHPETARNYRDPDFLALAA
                     REAWLQYGLDMSEYVYISFFNPDYTGEVTLSVRVDGRWYYLHDALGTGITTHGYRRVA
                     IYVGSRPLPDDIEEVRIITRTSRHVPLEVSAVYVFPRPSIES"
BASE COUNT          452 a          853 c          841 g          396 t
ORIGIN      
        1 atcgggacgg tcgatgcctg gcgggttgag gtcaccttcg aagccaatga cgccttcaaa
       61 gcgacttccc agaccggcgg catcatcgat cgctcgttca acttgcttca cgaaggcgaa
      121 agctggtact ggttccccgc cacgggcgag ctggactcct ttaaggaagg cagctgcggc
      181 ctaccctggc ccggccactc tcccgtgtag ggaccgatcc aggatcggct ctacacgggc
      241 gttccccagc atactgcctt gtgcttcaga caagcgtggc ctgcatcctg ctatagacat
      301 cgcaacctcg cgcatatcct ctcgtccttc tcgcttcctc tgggccatct tcagtgacaa
      361 ccttccacag ccggacgggg cgcgcgacct ctccggctgt ctgccgtcgc ctgaggatgt
      421 aaatctcgaa gcgaccgtag gtcgggtcgc tgatgatgac cggcggcgct gcccgttcgg
      481 ccaggaagac cgcgatctgt gcctgcagcg cagccgggta gcgcccgatc tcgctgcgtg
      541 cgttgtcgtc aatgatcagc gcgccggccc cggtcgcatc gaactgggct gcgaaggacg
      601 gcgtgccgct cgtctcctcc cacgtcaact ggagcacgtt gagcgcgcga tagtcgcgct
      661 cgtggagccc ccaccaccag cgctcatgtc cgatgatgcc ggttcccgct ggaatcgcgg
      721 cgtcgagttg ctgtgcgaca ttctcgtaat tcgagacggc ctgcgcccgc agccggtcgt
      781 cgcggtaggc gtcggctccg tcagcgacga tcacggcagc aacgacgagg gcgacgcagc
      841 aaccagcgac cagtagccag cgctcggcgg caggctggtc ttgcgtgaag cggccccgcg
      901 cccaggctat cccagcagcg ccggcggcgg cgatcgcgat acagcagccg ggccagagga
      961 cgatcacgta gagcggcgct ttcgtgctgt cgagcagtgc cagcgatccg gcgccgccga
     1021 gcagcaacgt cagcagcagc cggtcgccgg tccgctccgg ctgccgcgcg cgccagccca
     1081 gccacgcgat cagcgggacg atccccagca cgaacagcca gttgagcgcg ccgctcggag
     1141 ccgtcagtcc ggtctcgatg agatgccggt agcggtcgcc ctcatgcagc aggttgtcga
     1201 gatagaacgt ggggtccaga aagccggtgc gacccgcctt cagagccgcc tgcccgtgaa
     1261 agtcggccca gtggacagcc acgtagagca ggtacggcgc gacaaccgcc gtccagccaa
     1321 ccgcgatgcg ccatcccagc gagactcgct gtcgcccatg ccagcccagc gtgagggcga
     1381 tgatgacgac cgtgaacgcg ccgaagaact gcgtcagtgt cgcgagcccg gcgcagatgc
     1441 cggttagcca cggcgtccat gcctgtcgat ccggtccccg ctcgatcgcg cggtccaggc
     1501 agacgacggt gagcatcgtg aaggccacgc cggtcatatc gtagcggccg gtcctgccga
     1561 ccgccagcag cggcagtccg ggccagagat cgaccaggtt gacccgcagg ccggccagta
     1621 gcagcccgca gatgcccgcc gcgagcgcgc cataccagcg cagcgccagc caggtcgtcg
     1681 cccagaggac gaccagcgcg ctggcgaccg tcaccaatcg ggcggtgacg atcccggccc
     1741 cgaacaggct gaagctcagt gcctggagca actggtagac cggcaacgcg acgaagtagt
     1801 gctggtccgc gccgtggaaa ccggcgtaga ggtccgaccc gaacacgccc tcagtcgcca
     1861 gcttgtacga ggccgacatt acccacgagt cgtcaccact gacggtcggg taccgcccca
     1921 gatcgggcag cacgaaggcc agcagcgcgc aggagagcag gaacgtaaac gctgcccagc
     1981 ctagcacctc ggagcgcctg tcaacgcgga cggtcggacg tctcaacgtt gcggctcctc
     2041 aatctgaccg gttgacgcct gcgtgggctc gtccggcgat tgtagaggaa cgatgccgca
     2101 gtggagtcag ctctcgatcg acggtctcgg gaagacgtag accgccgaga cttcgagtgg
     2161 gacatgccgg gaggtgcggg tgatgatgcg gacttcttcg atatcgtccg gtagcgggcg
     2221 cgagccgacg tagatggcaa cgcgccggta gccgtgggtg gtgatgccgg ttccgagcgc
     2281 gtcgtgcagg tagtaccagc ggccatcgac gcgaaccgag agcgtcacct cgccggtgta
     2341 gtccgggttg aagaacgaga tgtagacgta ctcgctcatg tcgagcccgt actggagcca
     2401 tgcctcgcga gccgccagcg ccagaaagtc gggatcgcgg tagttacggg ccgtctcggg
     2461 atgcggcatc agcgccagca gccggtgttc cccggcccgt tcggcctcgt cctggccgac
     2521 atgccggaag ttgtggttgg cg
//
LOCUS       DASUGJ010000177         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_719331, whole genome shotgun sequence.
ACCESSION   DASUGJ010000177 DASUGJ010000000
VERSION     DASUGJ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719331"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..759)
                     /locus_tag="VFV93_03900"
     CDS             complement(4..759)
                     /locus_tag="VFV93_03900"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5164516.1"
                     /translation="MLQNKVALVTGAASGIGRATAVAFAKAGARAVAVADVNTEGLEE
                     TAAMVRALGAEALAIRTDVSRAEDVERMVADTVAAFRRLDCAFINAGIEGATAPTAEI
                     TRMDWDRTLATNLTGVWLCMKAELPLLLAADGGAIVNCASVAGLVGFQGAAAYVASKH
                     GVVGLTKATALDYAPTSIRINAVCPGVIQTPMIDRVLERDPAMEAALTAMEPAGRLGR
                     ADEIADAVIWLCSDAASFVTGQAIAIDGGFVAR"
     gene            1071..1790
                     /locus_tag="VFV93_03905"
     CDS             1071..1790
                     /locus_tag="VFV93_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5164517.1"
                     /translation="MATYWSRLVVVLLLVVSVISAACGTGDSGTSDDGDVRAVTVTGE
                     GRVSRAPDIVRMTLGVDISRPELGGAQSLAAETMTAVTAMLQERGVAGDDIQTETYAI
                     YLDRDDSKSSQPVAGYHVVHTVTATVRDVAQAGEILTAAIDAGANNVQGISFAREDDA
                     AAIAEARALAVADARAKAEDLARLADVELGPVITLSESVTGSSPYRDAGESAGGGVVP
                     ISPGQAVVGVSVTIEWELTGD"
     gene            1886..>2488
                     /locus_tag="VFV93_03910"
     CDS             1886..>2488
                     /locus_tag="VFV93_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5164518.1"
                     /translation="MIDRIVVPLDRSALSEIAIPFATTLAGMVGARIEYLTVVPEQED
                     LDTGGADRYLLKIAERHPPSTDFRIVVRMGSAADVIVDEADHPGAMIVMATHGRGGLQ
                     RMLVGSIADIVVRTATVPVALIRDGVGMGTVLEPFSHILVPLDGSDRSATSLPLAIEL
                     ARRSGAHLHLLQVVPPVQVNEIGYASDASLLSPDVYADMMD"
BASE COUNT          447 a          819 c          782 g          440 t
ORIGIN      
        1 cgcttatcgc gcgacgaacc caccgtcgat cgcaatcgcc tgcccggtca cgaagctggc
       61 ggcgtccgaa cacagccaga tgaccgcgtc cgcgatctcg tcggctcgcc cgagccggcc
      121 agccggctcc atcgcggtca gcgcagcctc catcgcggga tcgcgctcca ggacgcgatc
      181 gatcatcggc gtctgaatca cacccggaca gaccgcattg atgcgtatgc tcgtcggggc
      241 atagtcgagg gccgtggctt tggtcagccc gaccacgccg tgcttgctcg cgacgtaggc
      301 ggccgctccc tggaatccga ccagccccgc caccgacgcg cagttcacga tcgcgccgcc
      361 gtctgccgcc agcaggaggg gaagttccgc cttcatacac aaccagacgc cagtcaggtt
      421 ggtcgccagt gtccgatccc agtccatcct ggtgatctct gccgttggag ccgtcgcccc
      481 ttcgatcccg gcgttgatga aggcacaatc gagccgtcta aaggcggcga cggtgtctgc
      541 caccatccgc tcgacatcct cggctcgtga gacgtcggtt cggatcgcca gtgcttccgc
      601 cccgagggcg cgaaccatcg cagccgtctc ctccagccct tcagtgttga cgtcagcgac
      661 ggcgacggcc cgcgcgcctg ccttagcgaa agcgacggct gtcgcgcggc caattcccga
      721 cgcggcgcca gtgacgaggg ccaccttgtt ctgaagcatc tggtactcct tgcactcacc
      781 atctgagccg gacagcggcg gcgacaacga gacgccagcg tctccccgga atccacgcca
      841 tcagaggatc actcatctgc cgcgacacag gcgtcacatc gcaacagctt ggcgtaacag
      901 caccatgtca atcggtgcga attcgtcgtt cctggcggtt gcggccggca tgacacggaa
      961 ctgtcacgca gacccagccc gcgttacgtg tttgagacag cgtctgacgt atacggatgc
     1021 tgtagacgca tcgacctgac gagcgtccga cgcgagggtg aggcggcacg atggcgacat
     1081 actggtcccg gctcgttgta gtgttgctgc tggtggtcag cgtcatcagt gcggcatgcg
     1141 gaaccggaga ctccgggacg agtgacgatg gcgacgtacg ggcagtgacg gtcaccggcg
     1201 aaggccgcgt gtcacgagcc cctgacatcg tcaggatgac gctcggcgtc gacatctcac
     1261 ggccggaact gggcggagca cagtccctcg cagccgagac gatgacagcc gtcactgcaa
     1321 tgctgcagga gcgcggtgtc gcgggcgacg atatccagac ggagacctac gcgatctacc
     1381 tcgaccgcga cgactcaaag tccagccaac cggtagccgg ctatcacgtg gttcataccg
     1441 tgaccgcaac cgtccgagat gtcgcccagg ccggcgagat cctgacggcg gccatcgatg
     1501 ctggcgctaa caacgtacag ggaatctcgt tcgcgcgcga ggacgatgcg gcggcgatcg
     1561 ccgaggctcg cgcactcgct gtcgccgacg cccgtgccaa ggccgaagac cttgcacgac
     1621 tggctgatgt cgaactcggc cccgtcatca cgctcagtga gagcgtgaca ggttcctcgc
     1681 catatcgtga cgcgggtgag tcggcaggcg gcggggtcgt ccccattagt cccggccagg
     1741 cggtcgtcgg cgtgagcgtc acgatcgaat gggaactgac cggtgactga tccgtgtcgc
     1801 tcccactccg aggcggccgt cccatgtttc atcaacgagg ggaatggatt cggtgaatcc
     1861 aggtggtgct agagaggaga gagccgtgat cgatcggatc gtcgttccgc tcgaccggtc
     1921 cgcgttgtcg gagatcgcca tcccgtttgc gacaacgctc gcgggaatgg tcggcgcccg
     1981 catcgaatac ctgaccgtcg tccccgagca ggaagacctt gataccggcg gtgcggaccg
     2041 gtacttgttg aagatcgccg agcggcatcc gcccagcacg gactttcgca tcgtcgtgag
     2101 gatgggtagt gcggccgatg tcattgtcga tgaggccgac cacccaggcg cgatgatcgt
     2161 gatggcgacg cacggccgtg gcggcttgca acgaatgctg gtcggtagta tcgccgatat
     2221 cgtcgtcagg acagcgaccg ttccggtcgc gctcatccgc gatggtgtcg gaatggggac
     2281 ggttctcgag ccgttctctc acattcttgt tccgctggac ggttcggacc gctcagcaac
     2341 ctcgctaccg ctcgccatcg aactagctcg ccgtagcggc gcgcacctcc atctgctcca
     2401 ggtcgtcccg ccggtccaag tcaacgagat cggttatgcg tcagatgcat cacttctcag
     2461 tcccgatgtc tacgcagaca tgatggac
//
LOCUS       DASUGJ010000178        10396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_302239, whole genome shotgun sequence.
ACCESSION   DASUGJ010000178 DASUGJ010000000
VERSION     DASUGJ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302239"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VFV93_03915"
     CDS             complement(<1..351)
                     /locus_tag="VFV93_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164519.1"
                     /translation="MVIVRAPMRISFGGGGTDISSYYTQYGGFVVSTAIARYASVMAY
                     PRGDGQIHITSADYGQREAFRRGDIPPVSEPLALPKAAIARFARHGLAATGIDMLLTS
                     QVPPGTGLGSSSAMA"
     gene            complement(385..1122)
                     /locus_tag="VFV93_03920"
     CDS             complement(385..1122)
                     /locus_tag="VFV93_03920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5164520.1"
                     /translation="MEQLTGRVAVITGGGSGLGEATASVFAEAGFAVACLDLNQQNAE
                     RVALLLVEQDYQATAVGCDVSDEQSVAAAIERVAGEFGRIDAVINCAAVDYVVSVNEM
                     HVEQWDRVLDVNLRGPFLMAKAVWPYMQRQGSGHIVNIASTAATRAWGNAAAYHASKW
                     GLVGFSRGLGVEGRDHGIKVTTVIPGGMRTHWFDSFPERGIPMPDEAHLQDPRNVAEV
                     ILFAVQMPAPSSMQEVIVTPVTETSWP"
     gene            complement(1151..2461)
                     /locus_tag="VFV93_03925"
     CDS             complement(1151..2461)
                     /locus_tag="VFV93_03925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5164521.1"
                     /translation="MVMIPAAVHRRVAFLSEHASPLALLGSVDAGGQNVYVDEVSRNL
                     VRHGYAVDVFTRRDDPGNQNVVDWAPGVRVINVDVGPSTFVPKDDLWPLMPAFRDAVE
                     RFAGRTGTRYDLLHGNFWMSGWVAARLGERFGIPVVQIFHATGRTKRRHQQAADTSPA
                     ERIAVEHDVIKSVDRIIAQCPAEETELVADYGADPATVVVIPSAVNIERYRPVDKAEA
                     RRRIGLPNGERVIGYVGRMLPRKDPRNIVRALAILNRELACPATLLLVGGETRDPDPI
                     ATPEIGEIQRLAAELGIADRLRFTGKRQPDELRNYYSASDVAVTTPWYEPFGLTPLEA
                     MACGTPVIGSAVGGITYTIADDETGLLVPPRDPDALARALHAILANADYRARMGRAAR
                     QRVEREFTWDTVASRTAALYDHLLLEQVAGTGVFADTLSRRIAG"
     gene            complement(2452..3444)
                     /locus_tag="VFV93_03930"
     CDS             complement(2452..3444)
                     /locus_tag="VFV93_03930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5164522.1"
                     /translation="MRILIWHIHGSYLNTLGRIDHDWFLPTKPDKPEGYYGRGETFDT
                     PPNMIPVPAERVRDLDLDAIVFQTPRNYTTDAEEILSAEQRRLPRIYLEHNTPKPDAV
                     NQRHPVDDPDVLLVHVTQYNRLMWDNGETRTVVIEHSVGIDPSIRYEGTLEAGIMVAN
                     GIQRRPRIAGYDLFLAMREQIPLDAAGMQTEEFGGLGDIPYRDLHGRVAAYRFLFSPM
                     RYTSLPLAVIEAMTIGMPVVTLATTELPTVIQHGENGFVTCDPDELLDCARLLLADPG
                     EARRIGDNARRTASERFALERFVDGWNQAFATVTSIPIDQFNPKPLRLEGGRWS"
     gene            complement(3441..4532)
                     /locus_tag="VFV93_03935"
     CDS             complement(3441..4532)
                     /locus_tag="VFV93_03935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5164523.1"
                     /translation="MSEVAPRRIAVFRALFLGDMLLSVPALRSLRRRYPAAEITLIGL
                     PWAASFAERFGHLVDRFLPFPGFPGIDEVAYDEQRTTAFMTEQRDYRYDVVIQMHGSG
                     RTSNPFAAALGGKVTVGYYVGDPPVGLDRAICYPDLAHEIGRNLWLAELAGGSAADRA
                     LEFPVSDADRYEADALTGPPSLRPLIVIHPGSKLPTRRWMPERFAAVADAVVERFGAE
                     VVVTGTDGEAAVADAVVSSMRHRAASLAGRTTLGSLAAIVERSRLFITNDTGTAHLAV
                     ALRTPSVVLFGPGDVRRWGPLDRERHRVVQVPTACSPCEHAYCPIDHRCLRAIEPEQV
                     LAEVSRVLVGHLDDWQLWPETEMRAPCAG"
     gene            complement(4529..5653)
                     /locus_tag="VFV93_03940"
     CDS             complement(4529..5653)
                     /locus_tag="VFV93_03940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5164524.1"
                     /translation="MIDARWQEARRVLIVRLDNLGDVLVTTPAIHAIRQALPEAQITL
                     LASPVGAQAGRLNADIDDVIEYQAPWMDPWHDLPLDPEREQAMIARLHDGRFDGAIIF
                     TSFRQSPLPTAYLCYLANIPLRVAASIDGPGSLLTTRHKHPEQMMHEVERGLDLVGAI
                     GMGTDDRRLRLAVPDDARDEALAALALCALDLSRPLVVVHPGCSMPARTYAWERFSEV
                     SQQLVEEEAATVILTGVASERELTEQVRGRVKGTARKLVLSMAGQLSFPGLCAMIEAA
                     DLTITNNTGPMHVSAAVGTPVVALFALTNPPEQWGPWQVPHRLLNHDVPCRICYSRVC
                     PYGHECLSLVTPDQVVAAARELLPMVATVGVPPRLAEVSA"
     gene            complement(5646..7139)
                     /locus_tag="VFV93_03945"
     CDS             complement(5646..7139)
                     /locus_tag="VFV93_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5164525.1"
                     /translation="MPSLVEVVRKFRGLRIVCIGDAMLDIYRFGSAARLCTEGPVPVV
                     VESGREQSPGGAANTAANLSALGAEIRFVGIVGEDDAGTNLRRSLTSYGVSDRWLVEE
                     AGVSTLCKERLIADGQFVARVDTGSTGHCSSRGITRLVENLRTALAGCDAVIVSDYNY
                     GGVPDAALDALRAWRSASDSPLVIDSKNLRRFSGAGANVVTPNLHEARALVADRVYGT
                     TNVAVVGRALLDALGCGHVAITTGADGVLLVGRDGSDVHIPAHPIATPADIGAGDSFA
                     AALTLALASGARIVDAAAIGIDAAGIAVARPGTAIVDHQELLQRVSLRGIAIGEFAAT
                     GEAERLADKLLVDRLGGKTIVFTNGVFDILHAGHVAFLREARALGDVLVVGVNSDASL
                     VRLPAQRRPINAERDRRSLIAALDLVDHAILFDDPTPADMIRLLRPHVHVKGGDYAAE
                     ELPEAEAVREVGGRVVILPLAGEAAEGAMIDRLVSVAASGALGAIHD"
     gene            complement(7123..7785)
                     /locus_tag="VFV93_03950"
     CDS             complement(7123..7785)
                     /locus_tag="VFV93_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5164526.1"
                     /translation="MTMNRAIFLDRDGTLVHARHYPSRPADLILYDGIGPRLRRLHEI
                     GFKLFVVTNQSGIARGYFTEQDLTAMHDHLSRELETLGVSVDGFYHCPHHPDGVIEHL
                     AITCDCRKPQPGMIRRAARDHGIELEWSWFVGDILDDIQAGNTAGCATVLVDLGTESR
                     PTSPIRRPDFVARDTPHALDIILFVEGVGPATSRDYWPSSWLINDDVAPLEPEVADAV
                     AG"
     gene            complement(7782..8465)
                     /locus_tag="VFV93_03955"
     CDS             complement(7782..8465)
                     /locus_tag="VFV93_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5164527.1"
                     /translation="MHEVASQNDHVVAVAPTAAIEDAAVAHQVDVLLHERLHSMAGAV
                     ACLAGRSGSIAAIASALVTTLRTGGKVLVAGNGGSAAEAQHFAGELVGRFLIEREAYA
                     AIALCVDSAVVTAIANDYGYDNVFARQVQGLGRPGDLFLAISTSGSSTNLIRAANVAR
                     DRGLRVAAITGDRANPLEEIANYTLRIPATSTPLVQELQMMVTHILCGIAETEMSSRA
                     DVNGERRRG"
     gene            complement(8613..9854)
                     /locus_tag="VFV93_03960"
     CDS             complement(8613..9854)
                     /locus_tag="VFV93_03960"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5164528.1"
                     /translation="MLNSVSDTPSSGHVALSAGQFENVAETITKRTATLLHADVTVTN
                     ARGIVVASSVQDIVGLQYGSASDGGDATFLRFPIRLDSHQGEVIVARPASQNGLPLEA
                     AQTLIDLLADQAAIGTRLPNQYELKNKFIHDLVMGAFSDTVDLRREGQILGMDLSAPR
                     AVVLVDASEYVLRAAGGRPAGEPSVEHEGSSIRRRAQHVIASVVSFFNLPDDTICGYI
                     GNGEIAVLKASADRDLSDWMSDDDRSRPTASLANLNALKRAGAALLTCIRHDTRLPVN
                     VGIGRYHAGIRGLSHSYQDASLAVQLGKRLHGDDRVHCLDSLGVAAFVGVSDPRTKLD
                     LAEHLLRPLDGEPELLRTLQSFFDEDCCPSSTAARLEIHRNTLAYRLERIATLLNLNP
                     RQFDDAVQLRIALLVRTFQHN"
     gene            complement(9855..10064)
                     /locus_tag="VFV93_03965"
     CDS             complement(9855..10064)
                     /locus_tag="VFV93_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164529.1"
                     /translation="MDNTRDASRHRPGVAPGASMAAKLAGGDPTPEVQLDPVSVSGER
                     VSPPVRVMIGNVIVVLGAAMLAAVI"
BASE COUNT         1815 a         3375 c         3377 g         1829 t
ORIGIN      
        1 ggccatcgcg ctggacgaac ccagtccggt tccgggcggc acctgcgaag tgaggagcat
       61 gtcgatcccg gtcgcggcca gcccgtggcg tgcgaatcga gcgatggcag ctttgggcag
      121 tgcgagtggc tcgctgacgg gcggaatgtc gccgcgtcga aacgcctcgc gctgtccgta
      181 gtcagctgac gtgatatgga tctgcccgtc gccgcgcggg taggccatga ccgacgcgta
      241 acgtgcgatg gccgtgctga caacgaagcc gccgtactgg gtgtaatagc tcgaaatatc
      301 ggtcccgccg ccaccaaagc tgatccgcat cggcgctcga acgatcacca tctgcccggt
      361 tccttccttc gaaacctgcg ccgtttacgg ccaactcgtc tcagtgacag gcgtgacgat
      421 gacctcctgc atcgacgacg gcgccggcat ctggacagca aacaggatga cctccgcgac
      481 gttgcgcggg tcctgaaggt gcgcctcgtc cggcatcggg atgccccgct ccggaaagct
      541 gtcgaaccag tgcgtccgca tcccgccggg gatgacggtc gtgaccttga tgccatggtc
      601 tctgccctcg acgcccagcc cgcggctgaa gccgaccagg ccccacttcg aggcgtggta
      661 ggcagcagca tttccccatg ccctcgtcgc agccgtcgag gcgatgttga cgatgtgtcc
      721 gctgccctgt ctctgcatgt atggccagac ggccttggcc atcagaaatg gtccacgtag
      781 attgacatcg agaacccggt cccactgctc gacgtgcatc tcgttgactg aaacgacgta
      841 gtcgaccgca gcacagttga tgacggcgtc gatccggccg aactcgccgg caactcgctc
      901 gatcgccgca gcgactgact gctcgtcgct gacatcacac ccgaccgctg ttgcctggta
      961 atcctgttcg accagtagca gagcgacccg ttcggcgttc tgctggttga ggtcgaggca
     1021 ggcgacggca aagccggcct cggcgaacac gctcgcggtc gcttcgccga ggccgctccc
     1081 accgccggtg atgaccgcga cgcggccagt tagctgttcc atgactcaac ctcttctctc
     1141 aacgcagaac tcagccggcg atccgtctcg acagggtgtc ggcgaacacg ccggttccag
     1201 ccacctgctc cagcaacagg tggtcgtaga gcgcggcagt tcggcttgcg actgtgtccc
     1261 aagtgaactc acgttcgacg cgctggcgtg cggctcgacc cattcgcgct ctgtagtcgg
     1321 cattggcgag gatcgcgtgc agcgcccgcg cgagcgcgtc cggatcgcgg ggaggaacca
     1381 gcaatccggt ctcgtcatcc gcaatggtgt aggtgatgcc accgacggcc gaaccgatca
     1441 ctggcgtgcc gcaggccatc gcttcgagcg gcgtcaatcc gaacggctcg taccatgggg
     1501 tcgtcacggc aacatcgctg gcgctgtagt aattgcgcaa ctcgtcaggc tggcgtttgc
     1561 cggtaaagcg cagccggtcg gcgatgccta actcggctgc caaccgctgg atctctccaa
     1621 tctcaggcgt tgcgatcgga tcgggatcgc gtgtctcgcc gccgacgagc agcagcgttg
     1681 ccggacacgc cagctctcgg ttgagaatcg cgagcgcgcg gacgatgttg cgagggtcct
     1741 tgcgcggcag catccggccg acatagccga tcacccgttc gccgttgggc aggccgatgc
     1801 gtcgtcgcgc ttctgccttg tcgactggcc ggtagcgttc gatgttgacg gctgagggaa
     1861 tcacgacgac ggtggccggg tcggcgccat agtctgccac cagttcggtc tcctcggcag
     1921 gacactgggc gatgatccgg tccaccgact tgatgacgtc gtgctcgacg gcgatccgct
     1981 cggcggggct ggtgtccgcc gcctgctggt gccggcgttt cgtgcggccg gtcgcgtgga
     2041 agatttggac aacggggatg ccgaatcgct cgccaagccg tgccgcgacc cagccggaca
     2101 tccagaaatt gccatgcagc aggtcgtagc gcgtaccggt ccggccggcg aatcgctcaa
     2161 ctgcgtcgcg gaaagccggc atgagcggcc agagatcatc cttcgggacg aacgtcgatg
     2221 gtccaacatc gacattgatg acgcggaccc caggcgccca atcgacaaca ttctggttgc
     2281 ccggatcgtc gcggcgggtg aagacatcga cggcgtagcc gtggcggacg agattccggc
     2341 tgacctcatc gacgtagacg ttctgcccac cggcatcgac gctgccgagc agggccagcg
     2401 ggctggcgtg ctcgctcagg aatgcgaccc gccggtgaac agcagccggt atcatgacca
     2461 tcgaccaccc tccaggcgca gcggcttcgg gttgaactgg tcgatcggga tgctggtgac
     2521 tgttgcaaat gcctgattcc agccatcgac gaagcgctcc agcgcgaacc tctcgctggc
     2581 cgtccgccgg gcgttgtcac cgatccggcg tgcttcgcca gggtcggcca gcagcagccg
     2641 cgcgcagtcg agcagttcgt ccgggtcgca ggtcacgaac ccgttctcac cgtgctggat
     2701 gaccgtcggc agctcagtcg tcgccagcgt gacgactggc atcccaatcg tcatcgcctc
     2761 gatgacggcc agcggcaggc tggtgtagcg cattggagag aagaggaaac ggtaggcagc
     2821 gactctgccg tgcaggtcgc gataggggat gtcgccgaga ccgccgaact cttcggtctg
     2881 catcccggca gcgtcgagtg gaatctgttc ccgcatcgcg agaaacaagt cgtagccggc
     2941 gattctcggg cggcgttgga tgccgttagc gaccatgatg ccggcttcga gcgttccctc
     3001 gtagcggatc gatgggtcga tcccaaccga gtgctcgatg acgaccgtcc tggtctcccc
     3061 gttgtcccac atcaggcggt tgtactgggt gacgtgaacg agcaggacat ccggatcatc
     3121 gaccgggtgc cgctggttga cggcgtccgg cttcggcgtg ttgtgctcga gatagatgcg
     3181 cgggagccgc cgctgctcgg cggagaggat ctcttccgca tcggtcgtgt agttgcgggg
     3241 cgtctggaag acgatcgcgt cgagatcgag gtcgcgcact cgctcggccg gcactgggat
     3301 catgttgggc ggcgtatcga acgtctcccc acggccatag tacccctcgg gcttgtccgg
     3361 cttcgtgggc aggaaccagt cgtggtcgat tctcccgagt gtgttcaggt agctgccgtg
     3421 aatgtgccaa atcagaatcc tcaacctgcg catggcgccc gcatctcagt ctcaggccag
     3481 agttgccaat catcgagatg gcccaccagc acccggctca cctcggccaa cacctgctcg
     3541 ggctcgatcg cgcgaaggca gcggtggtcg atcgggcagt acgcgtgctc gcacgggctg
     3601 caagccgtcg gcacctgaac gacgcggtgc cgctcccggt cgagcggccc ccagcgtcga
     3661 acgtcacccg ggccgaacaa cacgacgctc ggtgtccgca gggcaaccgc caggtgagct
     3721 gtgccggtgt cgttggtgat gaacagtcgg gatcgctcga cgatcgccgc cagcgaaccg
     3781 agcgtcgtcc tgccggccag cgatgcagct cggtgtcgca tcgacgacac gacggcatcg
     3841 gcgaccgccg cctcaccgtc agtgccggta acgacgacct ccgcgccgaa ccgctcgaca
     3901 acggcatcgg cgactgcggc gaaccgctcg ggcatccagc gtcgtgtcgg gagcttcgag
     3961 ccgggatgaa tgacgatgag gggccggagg ctgggaggac cggtcagcgc atcggcctca
     4021 taccggtcgg catcactgac cgggaactcc agcgcgcggt cagccgccga acctccggcc
     4081 agctccgcca gccagaggtt gcgaccgatc tcgtgggcca ggtcggggta gcagatggcg
     4141 cggtcgaggc caaccggtgg atctccgacg tagtagccga ccgttacctt tccaccgagg
     4201 gcggccgcga acgggttgct cgttctgccg ctcccgtgca tctggatgac gacgtcgtag
     4261 cgatagtcgc gctgctcggt catgaacgct gtcgtgcgct gctcgtcgta cgcgacctca
     4321 tcgataccgg gaaagccggg aaagggtagg aagcggtcga ccaggtggcc gaatcgctcg
     4381 gcaaacgagg ccgcccacgg caagccaatc aacgtgatct cggccgccgg atagcggcgg
     4441 cgcaacgacc gcagcgccgg gaccgacagg agcatgtcgc caagaaagag ggcgcgaaag
     4501 acagcgattc ggcgcggcgc gacttcgctc atgccgaaac ctccgcaagc cgtggtggta
     4561 cgcccaccgt cgccaccatg ggaagcagtt cacgagcggc ggcgacgact tggtccggcg
     4621 tcaccagcga caggcattcg tggccatacg gacagacgcg gctgtagcag atccgacatg
     4681 gaacgtcatg gttgagcagc cgatgtggca cctgccacgg cccccactgc tcgggcgggt
     4741 tggtcagcgc gaagagcgcg acgactggtg tgccgacggc ggctgagaca tgcattggcc
     4801 cggtgttgtt ggtgatcgtc aggtcggctg cctcgatcat cgcgcagagt ccagggaatg
     4861 agagttggcc cgccatcgat aggaccaatt tacgcgccgt gcccttaacc cgtccgcgga
     4921 cttgctcagt caactcccgc tcgcttgcga caccggtcag gatgacagtc gccgcctctt
     4981 cctcgaccag ttgctggctg acctcggaaa atcgctccca cgcgtaggtc cgcgccggca
     5041 tcgagcaacc tgggtgaacg acgaccagcg gtcgggatag atcgagcgcg cacagcgcga
     5101 gagccgccaa tgcctcgtcg cgtgcatcgt ccgggacggc cagccggagc cgccggtcgt
     5161 cggttcccat cccaatcgcg ccaaccaggt caaggccgcg ctccacctcg tgcatcatct
     5221 gctcggggtg cttgtggcgg gttgtcagca acgagccggg gccgtcgatc gaggccgcga
     5281 cgcgcagagg tatgttcgcc aggtagcaga gataggcagt gggcaacggc gactggcgga
     5341 atgaggtgaa gatgatcgcg ccgtcgaaac ggccgtcgtg gagccgggcg atcatcgcct
     5401 gctcccgctc cggatcgagc ggtagatcgt gccacgggtc catccacggc gcctggtact
     5461 cgatgacgtc gtcgatgtcg gcgttgagcc gtcccgcctg ggcgccgacc gggctggcga
     5521 gcagtgtgat ctgcgcctcg gggagtgctt ggcggatggc atggatggcc ggcgtcgtca
     5581 cgagaacatc accgaggttg tccagccgga cgatgagcac ccggcgcgcc tcctgccagc
     5641 gagcgtcaat catggatcgc tccaagcgct ccgctggcag cgaccgagac gaggcggtcg
     5701 atcatcgctc cctcagccgc ctcaccggcc agcggcagga tcacgacgcg gccaccaacc
     5761 tcgcgaacgg cctcagcttc gggaagttcc tcggctgcgt agtcgccacc tttcacatgc
     5821 acgtgggggc gcagcagccg gatcatgtct gccggggtcg ggtcatcaaa caggattgca
     5881 tgatcgacca ggtcgagagc cgcgatcagc gaccgccggt cccgttcggc gttgattggg
     5941 cgtcgttgcg ctggtaatcg caccagcgag gcgtcgctgt tgacaccaac gaccagcacg
     6001 tcacccagag cacgcgcctc gcgcaggaac gcgacatggc cggcatggag gatgtcgaag
     6061 acgccattgg tgaagacgat cgtcttaccg cccagccggt cgacgaggag cttgtcggct
     6121 agccgctccg cctcgccagt tgcagcgaac tctccaatcg ctatcccacg caggctgacc
     6181 cgctgcagca actcctggtg atcgacgatg gccgtgcccg gcctggcgac ggcgatcccg
     6241 gccgcgtcga tgcctatcgc tgccgcgtcg acgatcctgg cgccgctcgc gagtgccagc
     6301 gtcagcgccg ccgcgaacga atcgcccgcg ccgatgtcgg ccggagtggc gatcgggtga
     6361 gcggggatgt gcacgtcgct cccgtcccgt ccgacgagca ggactccgtc ggctccggtc
     6421 gtgatcgcta catgtccaca gcccaatgca tccaggagcg cccggccaac gactgcgacg
     6481 ttggtcgtgc cgtagacgcg atccgcgaca agcgccctgg cttcgtggag gttcggcgtc
     6541 acaacgtttg ccccagcccc ggagaacctg cgcaggttct tcgagtcgat cacgagcggg
     6601 gaatcgctcg ccgatcgcca tgcgcgcaac gcatcgagcg cagcatctgg cactccacca
     6661 tagttgtagt cggagacgat gacggcgtcg cagccggcca gcgccgtccg caggttctcg
     6721 acgagccggg ttatccctcg cgacgagcaa tgaccggtgc tgccggtatc tactcgtgcc
     6781 acgaactggc cgtcggcgat caggcgctcc ttgcacaacg tactgacgcc tgcttcttca
     6841 accagccagc ggtcgctgac gccgtatgat gtcagcgatc gacgcaggtt cgttccggca
     6901 tcgtcctctc cgacgattcc gacgaagcgg atttccgccc cgagcgcgct caggttagca
     6961 gccgtgttcg cggccccgcc cggcgactgc tcgcggcccg attcaacgac gaccgggacc
     7021 ggcccttcgg tgcagagccg tgctgccgaa ccgaaccggt agatgtccag catcgcgtcg
     7081 ccgatacaga cgatgcgcaa ccctcggaac ttgcggacga cctcaaccag cgacggcatc
     7141 ggcaacctcc ggttcgagcg gcgcgacatc gtcgttgatc agccatgacg acggccaata
     7201 gtctcgcgac gtggctggcc cgacaccttc gacgaacagg atgatgtcga gtgcgtgcgg
     7261 cgtatctcgc gcgacgaaat ccggccggcg gatcggtgac gtcggccgcg attccgttcc
     7321 cagatcgacc aggactgtcg cgcagccggc agtgttaccg gcctggatgt cgtcaaggat
     7381 gtcgccgacg aaccaggacc actccagctc gattccgtga tcccgagcgg cgcgccggat
     7441 catgcccggt tggggtttgc ggcagtcgca ggtgatggcc agatgctcga tgacgccatc
     7501 cggatggtgc gggcagtggt agaagccgtc gaccgacacg ccgagtgttt caagctctcg
     7561 cgagaggtga tcgtgcatgg cggtgagatc ctgctcggtg aagtagccgc gggcgatgcc
     7621 ggactggttc gtgacgacga acagcttgaa cccgatctcg tgcaggcgtc gcaatcgtgg
     7681 cccgatcccg tcgtagagga tcaggtcagc cgggcggctc gggtagtgcc tggcgtgaac
     7741 aagcgtgccg tcccgatcga ggaagatggc ccggttcatg gtcatccgcg tcgcctctcg
     7801 ccgttgacat ccgcccgcga cgacatctcc gtctcggcga tgccgcacag gatgtgcgtg
     7861 accatcatct gtagctcctg aaccagcggt gtgctggttg ccgggatccg cagcgtgtag
     7921 ttggcgatct cttcgagcgg gttcgcccga tcgccggtga tcgccgcgac ccgaaggccc
     7981 ctgtcccgcg ccacgttagc cgcccggatc aggttggtgg aagaaccgct tgtgctgatc
     8041 gccagaaaca ggtcacctgg acggccgagc ccctgcacct ggcgcgcaaa gacattgtca
     8101 tagccgtagt cgttcgcgat cgcggtgacg acggcactgt cgacacacag cgcgatcgcg
     8161 gcgtacgcct ctcgctctat caggaagcgg ccaaccagct cgccggcgaa gtgctgcgcc
     8221 tcggcggcac tgccgccgtt cccggcaacc agcaccttgc cacccgtgcg cagcgtcgtg
     8281 acgagcgccg acgcgatggc agcgatcgat ccggagcgcc cggccaggca cgcgaccgcg
     8341 ccagccatcg agtgcaggcg ttcgtgcaac agcacatcga cctgatgtgc cacggcagcg
     8401 tcctcaatcg ccgcggttgg cgcaacggcg acgacgtgat cgttctggct agcgacttcg
     8461 tgcatgggag ctcgtccaga ttccatcgtg gaacgccagc ttgaccggca gaacgcaacg
     8521 tccctcaggt tagtgaactg ggaacgttgc gacattgggc gaaggcacaa gtgcgttcgg
     8581 cggacttcct gagtttttga gcggaagcac agtcagttgt gctggaacgt ccggacgagc
     8641 agcgcgatcc gcaactggac cgcgtcgtcg aactggcgcg ggttgaggtt gagcagtgtc
     8701 gcgatccgtt cgaggcggta cgccaaggta ttccggtgga tctccagcct ggcagcggtt
     8761 gacgacgggc agcaatcctc gtcgaagaac gactgcagcg ttcgcagtaa ctccggctct
     8821 ccatcgagcg ggcgcagcag atgttcggcc agatccagct tggttctcgg gtctgacaca
     8881 ccgacgaacg ccgccacgcc gaggctgtcg agacagtgga cgcggtcgtc tccgtgcaac
     8941 cgcttgccca gttggacggc tagcgatgcg tcctgatacg agtggctcag tccgcgaata
     9001 ccggcgtgat agcgcccgat gccgacgttg accggcaggc gggtgtcgtg tcgaatgcag
     9061 gtgagcagcg cagccccggc gcgtttgagc gcgttcaggt tcgccaacga cgccgttggc
     9121 cggctgcggt catcgtcact catccagtcg gacaggtcgc gatccgcgct ggctttcaga
     9181 actgcaattt cgccattgcc gatgtatcca cagatcgtgt catccggcag gttgaagaag
     9241 ctgacgacac tcgcgatgac atgctgcgct cgtcgtcgaa tactcgatcc ctcgtgctcg
     9301 accgacggtt caccggccgg ccggccgccg gcggcacgca ggacgtactc cgacgcgtcg
     9361 accagcacga ccgcccgtgg tgcggagagg tccatgccga ggatctgccc ctcgcgccgg
     9421 aggtcaaccg tgtccgagaa cgcccccatg acgaggtcat ggatgaactt gttcttcaac
     9481 tcgtactggt tgggcaaccg cgtcccgatc gccgcctgat cagccagcag atcgatcaac
     9541 gtttgcgcgg cttccagcgg gagtccattc tggcttgcgg gacgtgccac gatcacctcg
     9601 ccctggtgac tatccaagcg gatcgggaag cgcagaaatg tcgcgtcgcc cccgtcactt
     9661 gccgagccgt actgcaggcc aacgatgtcc tggaccgaac tggcgacgac gatgccgcgc
     9721 gcattggtga ctgttacgtc tgcatggagc agcgttgccg tccgcttggt gatcgtctcg
     9781 gcaacattct cgaactggcc cgcacttaac gctacgtggc cgctgctggg cgtatcacta
     9841 accgagttca gcatttagat caccgccgcc agcattgcag cgccaaggac aacgatcacg
     9901 ttaccgatca tgacccggac cggcggagat acacgctctc cactgactga gactggatcg
     9961 agctgaactt ccggcgtcgg atcgccccca gccagcttcg cagccatcga agcaccggga
    10021 gcgacaccag ggcgatggcg cgacgcgtct cgggtgttgt ccatcgtcgt gtcatccagc
    10081 gttagatgct gatcgcccgg ccgtgtttcc gtgaggctat cgtccctgat caacaggtcg
    10141 ggtggctggt tcccatttcg cattcgattc gacgtttctc gctctcgcgc gttggactgc
    10201 ttgtcagtca tccgcccgga acgattgtgt tcgtcattga gcattggagt ctccgataga
    10261 catagagtgc cgaccgcaag taactccgtt cactagcggg gttcgctgca ttgaactggc
    10321 ggtcgcgcat cgggaaactg gtaggcccct atgagattca acgtcgtgca gatgcacgat
    10381 cgatgccatg cggtcc
//
LOCUS       DASUGJ010000179         5578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_302331, whole genome shotgun sequence.
ACCESSION   DASUGJ010000179 DASUGJ010000000
VERSION     DASUGJ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302331"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1346
                     /locus_tag="VFV93_03970"
     CDS             198..1346
                     /locus_tag="VFV93_03970"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5164530.1"
                     /translation="MVSIAGARRGRVGSSISQPTEYDPFIVVLVIVLGLFGLVTIWSA
                     SGGGPITAGSQVVRQGFYLFTGLIAMFALSTVHYRFLKSFAAVFYLGSVALLVSLLVI
                     GSTEGGSTRWIMIGPLSLQPSEVAKIAVIIALAAFISERHDEMDRFSNFLISIAIVGL
                     PMGLVFIQPDLGTTGVFAAIWLGMILMSSTRMLYVAGLCLLSLPGAYVAWNFLMHDYQ
                     KDRLLIAFDPYKDYLGEGFNIIQAQVTIGSAGWFGHGLTGGSQSEFQLLKVRETDFIF
                     AHAMSMFGFVGGLALFFTFMLLLWRMLRVVNISPDTFGQQIAAGMTTMIFFQTFVNIG
                     MNLGLLPVTGITLPFISLGGTSLFVTLASFGIIQSIVVNHRRLGFQAG"
     gene            1460..3325
                     /gene="dnaX"
                     /locus_tag="VFV93_03975"
     CDS             1460..3325
                     /gene="dnaX"
                     /locus_tag="VFV93_03975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX5164531.1"
                     /translation="MLPGLGDADRTPGRTQSLYRKYRPTTFEDDELVGQEHIARTLRN
                     AIARGRVAHAYLFCGPRGTGKTSTARLLAKAVNCLNDDPAQRPCNACASCIAINNGSA
                     TDIVEIDAASNRGIDDMRDLRERVNYAPVQLKTKFYIIDEAHQVTKDAFNAFLKTLEE
                     PPPNTTFILATTDPDKLPETIASRCQRFDFRRIPRDGMIAHMRRIADREGIAIDDEVL
                     EIVARRATGSLRDGLSLIDMLATAAGEQAEGRIDIALARRMLGMTDDGWEYELIRALA
                     DRDITAGLAVIGNVVDAGHDMRSFGRRLLELLRVLMLVRAGADPVESNETIRELAPRF
                     ELPQLLAINRQFAEVDFKIRSGSFPQLPLELAFLGSLVEQPAAAAPVVAPVYAPSASA
                     SRPAMQERRPTEFQRRAPTEPRADRPAPAASPYEPEEQPRRQPAPQAQTPPPAPPSTP
                     RTSSGSADDSNLFQRIAAGWERIRTEVKTVDRKVEALLASADPGSVSGATLYVIAAYP
                     FHVGKLNEAKVRGIVEDAVERVVGERLHTSFVLRDELPGASAGAPPPAGPTAPSGWSR
                     QSDPPPAATSNGVYEDAPPFDDDIAPPDDDDFTRTVKATMNAEEVTDPEEIARIP"
     gene            complement(3420..4703)
                     /locus_tag="VFV93_03980"
     CDS             complement(3420..4703)
                     /locus_tag="VFV93_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164532.1"
                     /translation="MSIVCTPRRKPAARLVTLVTLLSILLTACQSGSDSADETATSYS
                     GPTVPTVIQMQATPTAAVTAEPTHSAALDALLAPGPTSPPPDGVFFQFGDAIWYQPGA
                     EPARPVVEGQRIGPWAQTSDGSRIALVRYRDENGQGVEELVLVNNDGSSGDPVYGPTS
                     ITGAGGGPTITALDWSWDGQSLALIRSDDTIWTMRFDPNDPFRTKPPLEPVSLPGSGA
                     PVALAWAPSGAGLAYTIAGESGTALYVTASGDPARTVIAPGGSPSRGVRTFDWLPGRG
                     RLVFVEVAGSQASHLPGSIFTIAPDGTLLELLVSAGQFAPAATISTLNAAPDGRFLAF
                     TVQVPDGLGQLTFQSLWLLTIDSGELRQVPIEAGYRVVDLAWSAGGLIWRGIDRNARV
                     PTDGRAYSGDEPFILGRFDPASGATSVVFQSTLVE"
     gene            4754..>5578
                     /locus_tag="VFV93_03985"
     CDS             4754..>5578
                     /locus_tag="VFV93_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5164533.1"
                     /translation="MSERRSLGRLAREPLYNTAAVVRATGIPAETIRAWERRYGLPRP
                     FRTPTRQRLYSEQDIGVISWLRERTDEGMTISQAIQRLQLEFPDVAGRPAAVQHPAPA
                     SVGDEIADLRRRLIEASAAFDSVAERIIDEALARLSIVTFCTSFVEPLLGELAERRNR
                     NDLNVAAGHVALRTLGRRLSALLPLVTPVRGRGNALVAAPSGVEQDAGSLMLALVLGQ
                     RGWRVVELGANVPADVLLAAADATRPDLICLRATTENAASQALSLAHLLMLQTDDPP"
BASE COUNT          894 a         1919 c         1799 g          966 t
ORIGIN      
        1 atccttcgct gcggctcagg atgacagggg tgagagatcg tgttcctcat cgacgccgtt
       61 catctgagca gcatcccgcc ccatgcacgc cacctgttcc ccacccgcta tactagtcac
      121 tgtcccgcgc ggtgactggg gttcgttggc tccggcgcgg ggtggtattc agccgctaac
      181 ggtggggtcg ttcaagcatg gtttcaatcg ccggagcacg gcgggggcgg gtcggatcga
      241 gcatcagcca gcccacggaa tacgatccgt tcatcgtcgt gctcgtcatc gtcctgggct
      301 tgttcgggct ggtcacgatc tggagcgcga gcggcggcgg gccgattacg gcgggctcgc
      361 aggtcgtgcg gcagggcttc tatctcttca ccggcctgat cgcgatgttc gcgctatcga
      421 cggttcacta ccgctttctg aagtcgttcg cggccgtctt ctatctcggc tcggtcgcgc
      481 tgctggtctc gctgctggtg atcggctcga ccgagggcgg ctcgacgcgc tggatcatga
      541 tcggcccgct ctccctgcaa ccctccgagg tcgccaagat cgcggtcatc atcgcgctgg
      601 ccgcgttcat cagcgagcgg cacgatgaga tggaccgctt ctcaaacttc ctgatctcga
      661 tcgcgatcgt cggcctcccg atgggcctcg tcttcattca gcccgacctc gggacgaccg
      721 gcgtcttcgc ggccatctgg ctcgggatga tcctgatgtc ctcgacgcgg atgctgtacg
      781 tcgccggcct ctgcctgctc tcgctgcccg gcgcttacgt cgcctggaac ttcctgatgc
      841 acgactacca gaaggatcgc ctgctgatcg cgttcgatcc ctacaaggac tacctgggcg
      901 aagggttcaa catcatccag gcccaggtga cgattggctc ggccggctgg tttggccacg
      961 gcctgaccgg cggcagccag tccgagttcc agttgctcaa ggtgcgcgag accgacttca
     1021 tcttcgccca cgcgatgagt atgttcggct tcgtcggcgg gctggcgctc ttcttcacct
     1081 tcatgctgct gctgtggcgg atgttgcgcg tcgtcaacat ctcgcccgac acgttcggcc
     1141 agcagatcgc ggccggaatg acgacgatga tcttcttcca gaccttcgtc aacatcggca
     1201 tgaacctcgg cctcctgccg gtgacgggca tcacgctgcc gttcatctcg ctcggcggca
     1261 cttcgctctt cgtgacgctt gcctcgttcg gcatcatcca gtcgatcgtc gtcaaccacc
     1321 ggcggctcgg gtttcaggcg ggatagccag gcggcagggc gtcgcacgac aggttcacgg
     1381 cccgcccaac acgggcggcg gctgacgggc tatacttgcc ctgaggtgta cggacactga
     1441 ccatggaaat ggaccgccga tgcttcccgg acttggtgat gcggaccgca cgcctggccg
     1501 cacgcagtcg ctctaccgca aatatcgccc gacgacgttc gaagacgacg aactggtcgg
     1561 ccaggaacat atcgcccgga cgctgcgcaa cgccatcgcg cgcggccggg tcgcccacgc
     1621 ctacctgttc tgcggcccgc gcggcaccgg caagacttcg accgcccgcc tgctggcgaa
     1681 ggccgtcaac tgcctgaacg acgacccggc ccagcgaccg tgcaacgcct gcgccagctg
     1741 catcgcgatc aacaacggca gcgcgaccga catcgttgag atcgacgccg cctcgaaccg
     1801 tggtatcgac gacatgcgcg acctgcgcga gcgcgtgaac tacgccccgg tccagctcaa
     1861 gaccaagttc tacatcatcg acgaggccca ccaggtcacc aaggacgcct tcaacgcgtt
     1921 cctcaaaacg ctcgaagagc cgccgcccaa cacgaccttc atcctggcga caaccgaccc
     1981 ggacaagctt ccggagacaa tcgcctcccg ctgccagcgg ttcgacttcc gccgcatccc
     2041 gcgcgacggg atgatcgccc acatgcggcg cattgctgac cgcgagggga tcgcgatcga
     2101 cgacgaggtg ttggagatcg ttgcccggcg ggcgaccggc agcctccgtg atggcctctc
     2161 gctgatcgac atgctggcga cggccgccgg cgagcaggcc gagggccgga tcgacatcgc
     2221 gctggcccgc aggatgctcg ggatgaccga cgatggctgg gaatacgaac tgatccgggc
     2281 gctggccgat cgggatatca ctgccggcct ggccgtgatc ggcaacgtcg tcgatgccgg
     2341 ccacgacatg cgctcgttcg gcaggcggct gctggagctg ctgcgcgtcc tgatgctggt
     2401 ccgggccggg gccgatccgg tcgagtcgaa tgagacgatc cgcgagctcg cgcctcgctt
     2461 cgagcttcct caattgctgg cgatcaaccg gcagttcgcc gaggttgact tcaagatccg
     2521 tagcggcagc tttccgcaac tcccactcga actggcgttc ctcggatcgc tggtcgagca
     2581 gccggccgca gcagcgccgg tcgtggctcc ggtctacgct ccgtccgcca gtgcgtcacg
     2641 tcctgcgatg caggagcgtc gcccgaccga gttccagcgc cgcgcgccga ccgagccgcg
     2701 cgccgaccgg cctgcgccag ccgccagccc gtacgaacca gaggagcagc cacgccggca
     2761 accggcgcct caggcgcaga cgccgccacc tgcaccgccc tcgactccgc gaacgagcag
     2821 cggttcggcg gatgactcga acctcttcca gcggatcgct gccggctggg agcgcatccg
     2881 caccgaagtc aagaccgtcg accgcaaggt cgaggcgctg ctggcgtcgg ccgatcccgg
     2941 ctcggtttct ggcgcgacgc tgtatgtgat cgccgcctac ccgttccacg tcggcaagct
     3001 gaacgaagcc aaggtccgtg gcattgtcga ggacgccgtc gagcgcgtcg ttggcgagcg
     3061 gctgcacacc tcgttcgtac tgcgcgacga gcttcccggt gcttcggctg gtgcgccacc
     3121 gcctgctggc ccaactgctc cgagcggttg gagccgccaa tccgacccac cgcccgctgc
     3181 cacatccaac ggcgtctacg aggacgcgcc cccgttcgat gacgacatcg cgccgccaga
     3241 cgacgacgac ttcacccgca ccgtcaaggc cacgatgaac gccgaagaag tcaccgatcc
     3301 ggaagagatc gcgcggattc cgtaagaagc ggtttctcgc ctcctgtttt gtcaaccttc
     3361 ggctcacgcc tcagtggccg accactcaga cgatatgtag agggggcgtg attgattcct
     3421 cattccacca gcgtcgactg gaaaacgacg ctggtcgcgc cgctggccgg gtcgaagcgg
     3481 ccgaggatga atggttcgtc gccggagtag gctctgccgt cggtcgggac ccgcgcgttg
     3541 cggtcgatcc cgcgccagat cagcccgccg gcgctccagg ccaggtcgac cacccggtag
     3601 ccggcttcga ttggcacctg gcgcagctcg ccgctgtcga tcgtcaacag ccacagcgat
     3661 tgaaatgtca gttgtccgag tccatccggc acctggaccg tgaacgcgag aaagcgacca
     3721 tccggcgcgg cgttgagggt tgagatcgtt gccgcaggcg cgaattgacc agcgctgacg
     3781 agcagttcca gcagcgtccc gtccggcgca atcgtgaaga tcgagccggg caggtggctc
     3841 gcctggcttc cggccacctc gacgaagacg agccggccgc gtcccggcag ccaatcgaac
     3901 gtgcgaacgc cccgcgacgg cgacccgcct ggtgcgatga ccgttcgtgc cgggtcgccg
     3961 ctggcggtga cgtagagcgc cgtgcccgac tcgccggcga tggtataggc gagaccggct
     4021 ccgctcggcg cccaggccag cgcgaccggc gcgccactgc ctggcaggct gaccggctcc
     4081 agtggcggtt tcgttcggaa cgggtcgttg ggatcgaagc gcatcgtcca gatcgtgtcg
     4141 tcgctgcgaa tcagcgccag cgactgcccg tcccacgacc aatccagcgc cgtgatcgtt
     4201 ggcccaccgc cggcaccggt aatcgacgtt gggccgtaga ccgggtcgcc gctgctaccg
     4261 tcattgttga cgaggacgag ttcctcgacg ccctggccgt tctcatcccg gtagcgaacc
     4321 agcgcgatcc gcgacccgtc ggacgtctgc gcccaggggc cgatccgctg tccctcaacg
     4381 accggccggg ccggttccgc gcccggctgg taccagatag catcgccgaa ctggaagaag
     4441 acgccgtccg gtggtgggct ggtcgggccg ggggcgagca gggcgtccag cgcggctgag
     4501 tgcgtcggct cggcggtgac ggcagccgtc ggcgtggcct gcatctgaat cacggttggg
     4561 acggtcggcc cgctgtaact ggtggcagtc tcgtccgccg aatcggaccc agactggcag
     4621 gcagtcagca ggatcgagag gagtgtgacg agtgtgacaa ggcgggcggc tggctttcga
     4681 cgcggtgtgc aaactatcga cacaacgtag acgccctgcc tagaatcggg acaccaaaca
     4741 ggaggaggtg cagttgagcg agcggcgttc actggggcgt cttgcacgcg agccattgta
     4801 caacacagcc gccgtcgtcc gggcgaccgg cattccggcc gagacgatcc gcgcctggga
     4861 gcgtcgctac ggcctgccgc gcccgtttcg gactcccacc cggcagcggc tctacagcga
     4921 gcaggacatt ggtgtcatca gctggctgcg ggagcgcacc gacgagggca tgaccatctc
     4981 acaggcgatc cagcgcctgc aactggagtt tcccgatgtc gccggccgcc ccgcggcggt
     5041 gcagcaccca gcacctgcta gtgtaggtga cgagatcgct gacttgcggc ggcggttgat
     5101 cgaggcgagt gccgccttcg actccgtcgc cgagcgaatc atcgacgagg cactggcgcg
     5161 actctcgatc gtgacgttct gcaccagctt cgttgagccg ctgcttggtg agcttgccga
     5221 gcgacgcaac cgcaacgatt tgaacgtcgc ggccgggcac gtcgcgctgc gcaccctcgg
     5281 acgccggttg tccgcgctgc tcccactcgt cacgccagtc cggggacgtg gaaatgcgct
     5341 cgttgctgcc ccgagcggcg tggagcagga cgccggctct ctcatgctcg cgctcgtgct
     5401 cgggcagcgg ggctggcgcg tcgttgagct gggcgcgaac gttcctgccg atgtgctgct
     5461 tgccgcagcc gacgccaccc gccccgacct tatctgcctg cgtgccacga ctgagaacgc
     5521 tgcctcgcag gcgctttcgc tcgctcattt gttgatgctg caaaccgacg atccgccg
//
LOCUS       DASUGJ010000180         4005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1413784, whole genome shotgun sequence.
ACCESSION   DASUGJ010000180 DASUGJ010000000
VERSION     DASUGJ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413784"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VFV93_03990"
     CDS             <1..933
                     /locus_tag="VFV93_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5164534.1"
                     /translation="LLKEIGRGNLSVGRVYEGHVNAIQLIQTFGTDVQLSRAATDALD
                     GRLFGVWNTEASDGVRITPLDGGRYRLDGAKTFASGAGHVQRPIVPGALPDGGWQMVV
                     VPMERVRVEIDPNWWQPIGMRASASYQVDFAGVEVDASDLIGQPGDYHRQPWFAGGAI
                     RFAAVQLGGAAALLDTMRDYLRGLDRTGDPHQRARAGQAAIAVETGNRWLAGAARQVD
                     LTPDAARSDDTTAAIAYANMTRTAIEQVCLEVMRLVGHSVGARGLLRPHPVERIVRDL
                     TLYLRQPAPDAALAEAGAFALESDERPSRHWCDG"
     gene            926..1519
                     /locus_tag="VFV93_03995"
     CDS             926..1519
                     /locus_tag="VFV93_03995"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009312 - oligosaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009877 - nodulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5164535.1"
                     /translation="MGEHDSLPAGYFERVYAENDDPWGFATRPYEARKYGATLAALPR
                     EHYRSAFEIGCSIGVLTEQLATRCDRLLAVDVAERALALARRRCRHLPHVRFDRMAVP
                     ARFPAESFDLILVSEVGYYWGWNDLRRAQRLIVEHLVPAGQLLLVHWTPYVADYPLTG
                     DQVHEAFLNGAKAGNRLHHLVGSRQQTYRLDLFERDG"
     gene            complement(1463..2509)
                     /locus_tag="VFV93_04000"
     CDS             complement(1463..2509)
                     /locus_tag="VFV93_04000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5164536.1"
                     /translation="MTRGHQSTSPFTPLVAPPPRPALRIAVVIPVRDEQECLPAALAA
                     LAGQRDLDGSPLDRDCYEIILLVNNSVDASAAIACVFARQDPSLALHVVEIDLPDDEA
                     HVGRARRMLMDAAAARLPWNGVIATTDADTRVAPDWVATTLAEVAAGADAVAGRIRVD
                     PTSFADDSGHARRLHLLDVTYRSLVDELEARLTPDPFDPWPRHFQHFGASMAVTVGAY
                     RRVGGIPPLSSLEDVALYDALRAVNARIRHSPAVRVVTSARPVGRTGFGFAVQLQRWS
                     AMGARHEPFLVEPPGAILARLAGQRSPAGPLVPIETAIADLRRLLAPLRREQARSAIT
                     LEQIEPVRLLAAAD"
     gene            complement(2511..3176)
                     /locus_tag="VFV93_04005"
     CDS             complement(2511..3176)
                     /locus_tag="VFV93_04005"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEX5164537.1"
                     /translation="MPRIVRDNALSLAMLVLFIVPLLGQSGTGHHVYNQEQREHGEAE
                     VSYLGYLKTGHFTESVFENWESEFLQMAMYVVMTAVLIQKGSAESKKGDGSDPQDADP
                     RQHQLDPNAPWPVRKGGAVLRVYEHSLALALFTLFILSFILHAAGGAKEYSAEQVAHG
                     GNEVRILEYMTTSQFWFESLQNWQSEFLAVFSIVVLSIFLRQRGSAESKPVHAPHAAT
                     GTD"
     gene            3411..>4005
                     /locus_tag="VFV93_04010"
     CDS             3411..>4005
                     /locus_tag="VFV93_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5164538.1"
                     /translation="MQELVQRLNGLCDEQPFHTGWYLKNLKTGEEADRNGQVILPSAS
                     TRKIAILAAALKAIHEGRLALDQPVEIQAKYQHNNSGTFQHLKPGFVITLQDAMMMMI
                     IISDNTCTGTVADLVGLDQINALCQSIGMTNTMHRFGIPPGNLTRDHAVDATNTTTPG
                     DVGLLLDLFQQATRDEAVAKRLGATPALCQLGIDILSW"
BASE COUNT          714 a         1347 c         1322 g          622 t
ORIGIN      
        1 ctgttgaagg agatcgggcg cggcaacctc tcggttgggc gggtgtacga aggacacgtc
       61 aacgccatcc agctcatcca gacgttcggg acggacgtgc agctgtcacg agcagcgacc
      121 gatgccctcg atggccggtt gttcggtgtc tggaataccg aagcgagtga cggcgttcgg
      181 atcacgccgc tggatggcgg ccgctaccgg ctggatggcg cgaagacgtt cgcctccggc
      241 gctggccatg ttcagcggcc gatcgtgccg ggagcgctgc cggacggcgg ctggcagatg
      301 gtcgtcgtcc cgatggagcg ggtgcgtgtc gagatcgacc ccaactggtg gcagccgatc
      361 ggcatgcgcg cctcggccag ctaccaagtc gacttcgccg gtgtcgaggt cgacgcctcc
      421 gacctgatcg gccaacctgg cgactatcac cgccagccgt ggttcgcggg cggcgcgatc
      481 cgcttcgcgg ctgtccagct cggcggggcc gccgcgctgc tggacaccat gcgcgactac
      541 ctgcgcggcc tggaccgtac cggcgacccg caccaacgtg cgcgggccgg tcaggcagcc
      601 atcgccgtcg agaccggcaa ccgctggctg gccggcgcgg cccgtcaggt cgatctgacg
      661 cccgatgccg cgcgcagcga tgacacaaca gcggccatcg cctacgctaa catgacgcga
      721 acggcgatcg agcaggtctg tctggaggtg atgcggctgg tcgggcactc ggtcggcgca
      781 cggggtttgc tgcgtccaca cccggtcgag cggatcgtgc gcgacctgac gctctacctg
      841 cgccaaccag cccccgacgc ggcactggcg gaagcaggcg ccttcgcgct cgagtcagac
      901 gagcgaccca gcaggcactg gtgcgatggg tgagcacgac tcgctgccgg ccggctactt
      961 cgagcgtgtc tatgccgaga acgatgatcc gtggggcttc gcgacgcggc cctacgaggc
     1021 acgcaagtac ggggcgacgc tggccgccct gccgcgcgaa cactacagat cggccttcga
     1081 gatcggctgc tcgatcggcg tcctgaccga gcaactggca acccgttgcg accggctgct
     1141 ggccgtcgat gtcgccgagc gggcgctggc gctggcgcgc aggcgctgcc gccacctgcc
     1201 acacgtccgt ttcgaccgga tggcggtccc ggccagattt ccggcggaat cgttcgatct
     1261 gatcctcgtc tcagaggtcg gctactactg gggctggaac gatctcaggc gggcgcagcg
     1321 gctgatcgtc gagcacctcg taccagcggg acagcttctg ctcgtccact ggacaccgta
     1381 tgtggcggac tacccactca ccggcgacca ggttcacgag gcattcctga acggagcgaa
     1441 agcgggaaac aggctccacc atctagtcgg cagccgccag caaacgtacc ggctcgatct
     1501 gttcgagcgt gatggctgat cgcgcctgct cgcgtcgcag cggggcgagc aggcggcgca
     1561 gatcggcgat cgccgtctcg atcggcacca gcggcccggc cggtgatcgc tgtcccgcaa
     1621 gtcgcgcgag gatcgcgccc ggcggctcga ccaggaacgg ctcgtgccgc gcccccattg
     1681 ccgaccagcg ctggagctgg accgcgaagc cgaagccggt ccggccgact ggtcgagccg
     1741 atgtcacaac gcgaacagcc ggcgaatggc ggatgcgggc gttgaccgca cgcagcgcgt
     1801 cgtagagcgc gacatcctcc agactcgaca ggggagggat accgccgact cgccggtacg
     1861 cgccgaccgt gacggccatg ctcgcgccga agtgctggaa atggcggggc cacggatcga
     1921 acgggtccgg cgtcagccgc gcttccagtt cgtccaccag ggagcgatag gtcacatcca
     1981 gcaggtgcaa ccggcgcgca tggccgctgt cgtcggcgaa actggtcggg tcgacccgta
     2041 tccggccggc aactgcgtca gccccggccg cgacctccgc cagcgtcgtc gcgacccagt
     2101 ctggcgcgac gcgcgtatcg gcatcggtcg tcgcgatcac gccattccac ggaagccgcg
     2161 ctgccgccgc atccatcagc atccgccggg cgcggccgac gtgcgcctcg tcgtcaggca
     2221 gatcgatctc gacaacgtgc agggcaagcg acggatcctg ccgggcaaac acgcacgcga
     2281 tcgcggccga cgcgtcgacg gagttgttga ccagcaggat gatctcgtag caatcccgat
     2341 cgagcggtga gccgtccaga tcgcgttggc cggccagcgc tgccaaggcc gccggcaagc
     2401 attcctgctc gtcgcgaacc gggatgacaa ccgcgatccg gagcgccggg cgaggcggcg
     2461 gcgcaacgag cggtgtgaat ggcgacgttg attgatggcc tctggtcatg tcaatcggta
     2521 ccggttgcgg cgtgcggcgc gtggaccggc ttcgactctg ccgagccgcg ctggcgcagg
     2581 aagatcgaca ggacgacgat cgaaaaaaca gccaggaatt cgctctgcca gttctgcagc
     2641 gactcgaacc agaattgtga ggtcgtcatg tactcaagga ttcttacttc gttgccgccg
     2701 tgggcgacct gctcggcact gtactccttc gcgccaccag ccgcgtgcag gatgaacgac
     2761 aggatgaaca gtgtgaacag cgccagcgcc agcgagtgct cgtatacacg cagcacggct
     2821 ccgcccttgc gaaccggcca cggcgcgttg ggatcgagtt gatgctggcg cgggtcggcg
     2881 tcctgtgggt cgctcccatc gcccttcttc gactccgctg accccttctg gatcagcact
     2941 gccgtcatga caacgtacat cgccatctgc aggaactcgc tttcccagtt ctcaaagacc
     3001 gactcagtga agtgaccggt cttcagatag ccgagatacg agacctcggc ctcgccatgt
     3061 tcccgttgct cctggttgta gacatggtgt cccgtcccgc tctggcccag cagcggaacg
     3121 atgaacaaga ccagcatcgc cagtgacaag gcattgtcac gcacgatccg cggcatgccg
     3181 accccttcca gcagactgac cgttgttcct ggcgtacgga catacccgtc gccagtagaa
     3241 ccctatgcaa accgtctgcc aacgacgttc gccccctctg tcatcctgaa gcgtgagccg
     3301 aaggatctct cctcgtgacg tcgagcggtg ggggagcgac agaaccacgg aaccacgcta
     3361 cgatggcccg cgccaccgaa tggcgagacg ggaacgaggg gagtacgacg atgcaggaac
     3421 tggtgcagcg gctgaacggg ctctgcgacg agcagccgtt ccataccggc tggtatctga
     3481 agaacctgaa gaccggcgag gaggccgacc gcaacgggca ggtgatcttg ccgtcggcca
     3541 gcacacgcaa gatcgcgatc ctggcggcgg cgctgaaggc gatccacgag ggccggctcg
     3601 cgctcgacca gccggtcgag attcaggcga agtatcagca caataacagc ggtacgttcc
     3661 agcacctgaa gccggggttc gtcatcacgc tccaggacgc gatgatgatg atgatcatca
     3721 tcagcgacaa cacctgcacc ggcaccgtcg ccgacctggt cgggctggat cagatcaacg
     3781 cgctctgcca gtcgatcggg atgacgaaca cgatgcatcg cttcggcatc ccgccgggca
     3841 acctgacccg cgaccacgcc gtcgacgcga ccaacacgac gacgccgggc gacgtcggcc
     3901 tgctgctcga cctgttccag caggcaactc gcgacgaggc cgtcgccaaa cgactcggcg
     3961 cgacgccggc gctctgccag ctcgggatcg acatcctctc gtggc
//
LOCUS       DASUGJ010000181         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1554358, whole genome shotgun sequence.
ACCESSION   DASUGJ010000181 DASUGJ010000000
VERSION     DASUGJ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554358"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3005
                     /locus_tag="VFV93_04015"
     CDS             <1..>3005
                     /locus_tag="VFV93_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEX5164539.1"
                     /translation="NPKSCTALDQCHTPGVCDPSNGVCSASPPKPDGATCDDGAACTQ
                     SETCVAGACQNTNNFPTVLNLPVQDMGSLGLIQSFATDINSSGTVVGWSSTNDVWIGH
                     AWTFSGNGPLVDMTELGLGPPSSADAINDAGTIAGYHTLDGVRHPFRYTVATGFEDLG
                     TYGDASNVLEAGQNIRGARASDVNNGGQVVGYYTLGNVFRGFRYSDGVYEDIESLAGG
                     GTVAAAISDTGRVVGSSWAVAGDSSSSHAIIYDNAEVGIIDLNSLIDPSSGWTLVSAT
                     DINGDFIVGNGIHNGADRAFRLRLPPGALTADVDEISGGWHVGPYATGVNAAGDVVGG
                     GYRTPENAAVSSWSAFVYTDALGFKNLDELIPHDFMWSWRVANAINAAGEIVGWGYHT
                     GEQGPRPFRIKLPAGQSASCQARNMCGGGDSDPVCLFSDGVVQNPDGTFVAVFGYDSA
                     STGNVTPTQNQAQLYNPDGNPQPAPPTLLAPGVHTGAYLPTFAEGQTVSWTVNGETVT
                     ASASSPRLQTVPDGNGGLAVLIGGVRLTIVPDLSPYDDPPAEPTAQDEPTLGPEFNGA
                     LTGELKVTPSGAATYTVPIKMPPGIAGMAPNLSLVYSSQSGNGIAGQGWSVGGLSYAH
                     RCPKNRVEDGEARPIMMNEVGDPDDPFTGDDEVCIDGQRLRGTVRDGMMEGAAHGGFF
                     TKTGEYRSYGSEIRSRVYGPNSSSPDDPNTRQVAIWALDKVSDVWGNYYEIHYNDDLL
                     TFYDSEDVQRRGLIVTRIDYTGNADGGGPDAFAPFHSIHFIYEEPDRPDVRTMRFHDF
                     VVPQRKRLSKIATPIGVYYLTYMPDDDPMLPSRLSSINYCAGTGCPETLPPPEQRRAA
                     GFVEPLEFDWEGGEYHWELEPDRGPDGHEDSYRLPRRIDVSGGYAHQGTQLADLNGDG
                     RPDFVESWFEGSARHAWENNGHGWTPRDGWALPATLIHGTDKEKSSTVMVDFNNDGFL
                     DVVSSQGTPLSIWINRIRANPSCTGATCW"
BASE COUNT          590 a          963 c          965 g          487 t
ORIGIN      
        1 caacccgaag agctgtacgg cgctggatca gtgccacacg ccgggcgtct gcgatccgtc
       61 gaacggcgtc tgctctgcga gcccgccaaa gccggacggc gccacgtgcg acgatggcgc
      121 ggcctgcacg caatcggaga cctgcgtcgc cggcgcgtgc cagaacacga acaactttcc
      181 gaccgtgctc aacctgccgg tccaggacat gggctcgctg ggattgatcc aaagctttgc
      241 gaccgacatc aattcatcag ggacggtcgt cggatggtcg tcgacgaacg acgtctggat
      301 aggtcacgct tggacgttca gcggcaacgg gccgctggtc gacatgacgg agctcgggct
      361 ggggccgccc agctcggcag acgcgatcaa cgacgcggga acgatcgccg gatatcacac
      421 gctggacggc gtgcgacatc ccttccgtta caccgtcgcc accggcttcg aggacctcgg
      481 cacctacggc gacgcctcca acgtgctcga agccggccag aacatacgcg gcgcgcgtgc
      541 aagcgacgtc aacaatggcg gacaggttgt cggctactac actctcggca acgtcttccg
      601 cggatttcga tacagcgatg gcgtgtacga ggacatcgag agcctcgcgg gcggaggcac
      661 cgtcgcagcg gccatctcgg acaccggccg ggtggtgggc tcctcttggg cggtcgcggg
      721 cgattcttcg agctcgcacg ccatcatcta cgacaacgcc gaggtcggaa tcatcgattt
      781 gaacagcctg atcgacccgt cgtccggctg gacgctggtg agcgcgaccg acatcaacgg
      841 cgacttcatc gtcggcaacg gcattcacaa tggggcggat cgcgcctttc ggttgcgact
      901 gccacccggc gccctcaccg ccgacgtcga cgagatctcg ggaggctggc acgtcggccc
      961 gtacgccacc ggcgtgaatg cagccggcga cgtcgtcggc ggcggctacc gcactcccga
     1021 gaacgccgcg gtgagctctt ggtcggcctt cgtctacacg gacgcgctcg gcttcaagaa
     1081 cctcgacgag ctgattcctc acgacttcat gtggagctgg cgcgttgcca acgccatcaa
     1141 cgccgcgggc gaaatcgtcg gctggggcta ccacaccggc gaacagggcc cgcgaccgtt
     1201 ccgcatcaag ttgcccgccg gccagagcgc gagctgtcag gcgcggaaca tgtgcggcgg
     1261 gggcgacagc gatccggtct gcctgttcag cgacggcgtg gtgcaaaacc ccgacggaac
     1321 gttcgtcgcg gtgttcggct acgacagcgc gtcgacgggc aatgtcacgc cgacgcagaa
     1381 ccaggcccag ctttacaacc cggacggaaa tccgcagccg gcaccgccca ccttgctggc
     1441 ccccggcgta cacaccggcg cgtacctacc gacgttcgcc gaaggccaga cagtgtcgtg
     1501 gaccgtcaac ggcgagaccg tcaccgcgtc cgcatcgagt ccgcgcctgc agaccgtgcc
     1561 cgacggcaac ggcggcctgg ccgtgctcat cggcggcgtt cggctgacga tcgtgccgga
     1621 cctgagtcct tacgacgatc cgccagccga gccgaccgcg caggacgaac cgaccctcgg
     1681 gccggaattc aacggcgcgc tgacggggga gctcaaagtg actccgagcg gagccgcgac
     1741 gtatacggtg ccgatcaaga tgccgcccgg tatcgcgggc atggccccga acctgagcct
     1801 cgtctacagc agccagagcg gaaacgggat cgcagggcaa gggtggagcg tcggcgggct
     1861 ctcgtatgcg catcgctgcc cgaagaaccg cgtcgaagat ggggaagccc gaccaatcat
     1921 gatgaatgaa gtgggggatc ccgacgatcc attcactggg gacgatgaag tatgcatcga
     1981 cggtcaacgg ctgaggggga cagtccgcga cgggatgatg gaaggggctg ctcatggcgg
     2041 cttctttacc aagaccggcg agtacaggtc gtacgggtct gagatcagga gtcgggtcta
     2101 cggtcccaac tcatcgtcgc cagacgaccc caatacacgg caggtcgcga tatgggcttt
     2161 ggacaaggtg tcggatgtct ggggcaacta ctatgagatt cactacaacg atgacctctt
     2221 aacgttttat gacagcgagg acgtacagag acgcgggcta atagtcacga ggattgatta
     2281 caccggaaat gctgacggag gcggacccga cgcatttgca ccgttccact cgatacattt
     2341 catctacgag gagcccgatc ggcccgacgt ccgcacgatg cgcttccacg acttcgtcgt
     2401 tccgcagcgc aagcgcctgt caaagatcgc gacacccatt ggtgtctact acctcaccta
     2461 catgcccgac gacgatccga tgttgccgag ccgcctctca agcatcaact actgcgcggg
     2521 cacaggttgt cccgaaaccc tgccgccgcc ggaacagcgg cgtgcagctg gcttcgtcga
     2581 gccgctggaa ttcgattggg agggcggcga gtaccactgg gagttggagc ccgaccgtgg
     2641 gcccgatggc cacgaggaca gctaccgctt gccacgtcgg atcgacgttt cggggggcta
     2701 cgcccatcag gggacgcagc tcgctgatct gaacggcgac ggccggccag actttgtcga
     2761 gagctggttc gaggggtcgg cgcgccatgc atgggagaac aacgggcacg gctggacccc
     2821 tcgtgacggg tgggctctcc cagcaacctt gattcacgga accgacaagg agaagtcgag
     2881 tacggtgatg gtcgacttca acaacgatgg cttcctcgac gttgtgtcta gtcagggcac
     2941 acccctctcc atctggataa accgaatcag agcaaatcct tcgtgcactg gagccacctg
     3001 ttggg
//
LOCUS       DASUGJ010000182         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1136086, whole genome shotgun sequence.
ACCESSION   DASUGJ010000182 DASUGJ010000000
VERSION     DASUGJ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136086"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VFV93_04020"
     CDS             complement(<1..986)
                     /locus_tag="VFV93_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX5164540.1"
                     /translation="MTNASGLLSSLPWRQAGPFRGGRSVAVAGDPSRPSTYYFGACAG
                     GVWKTDDGATTWRNVSDGFFRTASVGALAVADSDPNVVYAGMGESCIRGNVSHGDGVY
                     RSDDGGATWQHRGLENTRHIARVRVHPRDENLVYVAAFGHTFGPHEDRGVFRSRDGGL
                     SWEKVLYRNEHTGACDLIMDAQNPRVLYAALWEAHRSPWELVSGGEGSGIFKTTDGGD
                     TWTELTNNPGLPEGLKGRIGVAVSPANPQRVWAVIESKEGGLFRSDDGGATWTRTSDS
                     PDLRQRPWYYMHLFADPADAETVYVLNLAMWKSTDGGKNFVEIPEPHGDNHDL"
     gene            complement(1240..2799)
                     /locus_tag="VFV93_04025"
     CDS             complement(1240..2799)
                     /locus_tag="VFV93_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5164541.1"
                     /translation="MSTKPGTEPAYFIAQAIPYVNGRPHVGHALEAVLADAYARYHRQ
                     AGDDVFFLSGSDENSLKNVQAAEALGVPTQELVDRNVQVFQELYAALDLSNDDFIRTS
                     VDPRHIAGAKKIWAAMVAAGDVYTKSYSGLYCVGCEQFYTADELVDGRCPEHGTVPEL
                     IEEENTFFRLSRYQQQLHDLIASDRIKIYPTSRKNEVLRFIEAGLEDFSISRSRERAH
                     GWGIEVPDDPGQVMYVWVDALSNYITALEYASDGPAFARYWLGSRDRVQVIGKGILRF
                     HAVYWPAMLLSAGLPVPTHVVVHGYLTINGQKMSKSLGNVVDPIEVAAQYGADPLRYY
                     LLRDISPFNDGDFSVEKLLGRYRADLANDLGNLLNRTVSMIQRYRDGIIPAVQPEASG
                     AIEDELVQAIGVASARVELAMAEYNPQEALTAIWDIVTRANGYVEVTAPWQLAKEERD
                     GSQPPGRLDTTLAALAITLSRIAFLLEPFLPETSQKIAAQLGSPTVGPAPATGQRVAE
                     PQPIFPRFEDA"
BASE COUNT          518 a         1031 c         1086 g          562 t
ORIGIN      
        1 aggtcgtggt tgtcgccgtg cggctccggg atctcgacaa agttcttccc gccgtcggtc
       61 gacttccaca tcgccaggtt gaggacatag accgtttcgg cgtcggccgg gtcggcgaac
      121 aggtgcatgt agtaccacgg ccgctggcgc aggtccgggc tgtcgctcgt ccgcgtccag
      181 gtggcgccgc cgtcgtcgga gcggaacagc ccgccctctt tcgactcgat cacggcccag
      241 acgcgctgcg ggttggccgg cgagactgcg acgccaatcc ggcctttcag cccctccggc
      301 aggccggggt tgttggtcag ctcggtccac gtgtcgccgc catcggtcgt cttgaagatg
      361 ccgctgcctt cgccaccgct gaccagctcc cacggcgaac ggtgtgcctc ccagagggcg
      421 gcgtagagga cgcgcgggtt ttgggcgtcc atgatcaggt cgcaggcgcc ggtgtgctcg
      481 ttgcggtaga gtaccttctc ccacgacagc ccgccgtcgc ggctgcggaa gacgccgcga
      541 tcctcgtgcg ggccgaaggt gtggccgaag gccgccacgt agaccaggtt ctcgtcacgg
      601 ggatggaccc gcacccgcgc gatgtgccgc gtgttctcca gcccgcgatg ctgccaggtc
      661 gcgccgccgt cgtcggaacg gtagacgccg tcgccatgcg agacgttgcc gcgaatgcac
      721 gactcgccca tcccggcgta gacgacgttg gggtcggaat cggcgaccgc cagcgcgccg
      781 accgaggccg tcctgaagaa gccgtccgag acgttgcgcc aggtcgtcgc gccatcgtcg
      841 gtcttccaga cgccgccggc acaggcgccg aagtagtagg tcgacggtcg cgacggatcg
      901 ccggcgacgg cgacactccg cccgccgcgg aacggcccag cctggcgcca gggcaggctg
      961 ctcaacagcc cggatgcgtt ggtcatcgtc tgctccctcc ctattgggag gttgttcatc
     1021 acatccgatg ccacgcgatt tcgcggtgat cctgggggcg atccgggacg gtcaatcgtc
     1081 ggcaatgtag gggcgaatcg gtgattcgcc cgaacaattc ggccgcgttc agcggcccca
     1141 atgcgccaat tggaattggg atcggctgga cgccgatcga attggtaggc gaatcaccga
     1201 ttcgccccta cattgcgacg attggatgca ttgatagcgt caggcatctt caaagcgcgg
     1261 aaagatcggc tgcggctcgg cgacgcgctg gccggttgcc ggagccgggc cgaccgttgg
     1321 gctgccgagc tgggcggcga tcttctggct cgtctccggc aggaacggct ccagcaggaa
     1381 cgcgatccgc gacagcgtga tcgccagcgc cgccagcgtc gtgtcgagcc ggccgggtgg
     1441 ctggctgccg tcgcgctcct ctttggccag ttgccagggg gccgtcacct cgacgtagcc
     1501 gttggcgcgg gtaacgatgt cccagatggc ggtcagcgcc tcctgcgggt tgtactcggc
     1561 catcgccagc tcgacccgcg ccgaggcgac gccgatcgcc tggaccagct cgtcctcgat
     1621 cgcgcccgat gcctccggct ggacggccgg gatgatgcca tcgcggtagc gctggatcat
     1681 actgacggtc cggttgagca ggttgccgag gtcgttggcc agatcggccc ggtagcggcc
     1741 cagcagcttc tcgaccgaaa agtcgccgtc gttgaagggc gagatgtcgc gcaggagata
     1801 gtagcgcagc gggtcggcgc cgtactgcgc cgcgacttcg atcgggtcca ccacgttacc
     1861 gagcgacttg ctcatcttct gaccgttgat cgtcaggtag ccatggacga caacatgggt
     1921 cggcaccggc aaccctgccg agagcagcat ggccggccag tagacggcgt ggaagcgcag
     1981 gatgcccttg ccgatcacct gaacgcggtc gcgcgagccg agccagtagc gcgcgaaggc
     2041 ggggccgtcg ctggcgtact ccagggccgt gatgtagttc gagagggcat cgacccagac
     2101 atacatcacc tggccggggt cgtcgggaac ctcgatgccc cagccgtggg cgcgctcgcg
     2161 cgagcgggag atcgagaagt cctccagccc ggcctcgatg aagcgcagta cctcgttctt
     2221 gcgcgaggtt gggtagatct tgatccggtc actggcgatc aggtcgtgca actgctgctg
     2281 gtagcgcgag aggcggaaga aggtgttctc ctcctcgatc agttctggaa cggtgccatg
     2341 ctccgggcag cggccatcga ccagctcgtc ggctgtgtag aactgctcgc aaccgacgca
     2401 gtagagcccg ctgtagctct tcgtgtagac atcgccggcc gcgaccatcg ccgcccagat
     2461 cttcttcgcc cccgcgatgt ggcgcggatc gacgctggtg cggatgaagt cgtcgttgga
     2521 cagatcgagc gccgcgtaca gctcctgaaa cacctgtacg ttgcgatcga ccagctcctg
     2581 ggtcggcacc ccgagcgcct cggccgcctg gacgttcttg agcgagttct catccgaccc
     2641 ggacaggaag aagacatcgt cccccgcctg ccggtggtag cgcgcgtagg catccgccag
     2701 caccgcctcc agcgcatgcc cgacgtgcgg ccgcccgttc acatacggga tcgcctgggc
     2761 aatgaaatag gccggttccg tgcctggctt tgtgctcatc ctggcctcca gcgggttacg
     2821 acgaatgctc gtcaaaatga gaacgcaggc tgtgcagcct gcgtgaactt ctgattattg
     2881 gctacggatg cctcgccgat atgcataacg ctgcctgcat gtggcagcag tgtagcatgg
     2941 ggtcggtcac tggactcggc ggagtgcaac acaatggctg ccaaccgatt cggcgggcag
     3001 ccatgttcgt ctttcgtggg tttggttagc ggtgatcggc ctcgcggaac gcctggcgcg
     3061 aggacggcag cgcgatcagg gcaagaatca cgaagccgag cggcacgaac gcgatcagtt
     3121 cgccgaggat ccagccgttc ggcggatcga ggaggccggg aatcgcggtc agcgtgttga
     3181 tcgcggtcac cacgaac
//
LOCUS       DASUGJ010000183         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1554664, whole genome shotgun sequence.
ACCESSION   DASUGJ010000183 DASUGJ010000000
VERSION     DASUGJ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554664"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VFV93_04030"
     CDS             <1..574
                     /locus_tag="VFV93_04030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164542.1"
                     /translation="MLLWLIGAFAALNLLLDPIFAVVLPVYAEQHFGSAVRLGLMVAT
                     FGAGTVAGVVAFGVVGTRLPRRPTLLVAIVMAGLPIWPLALVDAFPVVIGALFVMGAA
                     IGPISPLVMTILHERVPAAFRGRTFGAMTAITSATIPLGIVITGFAIELLDLRMTLLV
                     LAAIYVAVAATSFVNPAFRQISQPLEEAAS"
     gene            complement(571..963)
                     /locus_tag="VFV93_04035"
     CDS             complement(571..963)
                     /locus_tag="VFV93_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5164543.1"
                     /translation="MTTRPLIAVVDDDRVYTEMIRDFLDGEGYDTVLLREASTAVEAI
                     VRARPALALLDMRMDVPQSGVAILADLRANPATSSLPVIICTADQQFLRTHAAELQRQ
                     NAAFVAKPFDLDVLLNVIEQTLAGHDGC"
     gene            complement(1107..>2171)
                     /locus_tag="VFV93_04040"
     CDS             complement(1107..>2171)
                     /locus_tag="VFV93_04040"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164544.1"
                     /translation="VVISLFFGGTLVDRLPYRRVSVVADLASGVSVLLIPFLHAFGLL
                     SFPLLLACIFAGALLDLPAGLARTAMLPDLARLSDMRTERAFAISEGATTVLGLATPA
                     LTGIFIAAVGASNVLWLDAASFGISALLMLAFVPETARDASRAMGGYLRELADGVRFV
                     RFEPVLLPLILFFAAMNLVIGPVDALIVPVYASEIFGSAVAFGLMAAASGAGALAGTA
                     LMGWIGHRLPRRAVFAGGFMAVPLALAALALTPALPLTLTVMALLGLALGMTNILEYT
                     VYFERIPEQMRARVMGLAGALGWWTVPLGRIVVGFLLEPLGLSSTLALLAVLFMPLPL
                     AIYLLRAFRDLGPPAATGAG"
BASE COUNT          394 a          715 c          731 g          331 t
ORIGIN      
        1 gatgctgctc tggctgatcg gcgccttcgc cgccctcaac ctgttgctcg atccgatctt
       61 cgccgttgtc ctgcccgtct acgcggaaca gcacttcggc agcgccgtcc ggctgggact
      121 gatggtagcg acgttcggcg ccggaacggt cgctggtgtc gtcgcctttg gggtggtcgg
      181 cacgcgcctg ccgcgtcgcc cgacgctgct ggtcgcgatc gtgatggcag ggctgccgat
      241 ctggccgctg gcactggttg acgcattccc ggtcgtcata ggtgcgttgt tcgtgatggg
      301 cgccgcgatc ggcccgatct ccccgctggt gatgacgatc ctgcatgagc gggtgccggc
      361 cgcgtttcgt ggccggacat tcggagcgat gacggccatc acgagcgcga cgatcccgct
      421 tgggatcgtc atcaccggct tcgcgatcga gttgcttgac ctgcggatga ccctgctggt
      481 tctggcggcg atctacgtcg ccgtcgctgc cacgtcgttt gtgaatccgg cgttccggca
      541 gatcagccag ccgctggaag aggccgcgag ctagcagccg tcatgaccgg ccagcgtctg
      601 ctcgatgaca ttcagcagta catcgagatc gaacggtttg gcgacgaagg cggcgttctg
      661 gcgctggagc tcggcggcgt gagtgcgcag aaactgctgg tcggccgtgc agatgatgac
      721 cggcaggctg ctcgtagccg ggttagcccg caggtcggcc agaatcgcga cgccggattg
      781 agggacatcc atccgcatgt cgagtagcgc cagcgccggc cgggcgcgca cgatggcctc
      841 gacggccgtc gacgcctcac gcagcagcac cgtgtcatag ccttcgccgt cgaggaagtc
      901 acggatcatc tccgtgtaga cccgatcgtc gtcgacaaca gcgatcaacg gtcgcgtcgt
      961 cacctgaaca cgccctttca gacagacgct ccagcggagc ggaccgagtg tcccgtccgt
     1021 cgcgggctgg attgctagcc ggtattccga cttcgttgta gcaggaattg tctgatcacg
     1081 caatcgttgg ccggctgtgc tgaacatcaa cccgccccgg tcgcagccgg tgggcccaga
     1141 tcgcgaaagg cgcgcaggag atagatcgcc aacggcaacg gcatgaagag cacggccagc
     1201 agcgcgagcg tgctggagag tccgagcggc tccagcagga agccaaccac gatccgccca
     1261 agcggcaccg tccaccagcc gagcgctccg gccagcccca tcacccgcgc gcgcatctgc
     1321 tcgggaatgc gttcgaagta gaccgtgtac tccaggatat tggtcatccc cagcgccagc
     1381 ccgagcagcg ccatgacggt gagtgtcagc ggcagggccg gtgtcaacgc cagcgccgcc
     1441 agcgccagcg gcacggccat gaagccgccg gcgaagacgg cccggcgcgg caggcgatga
     1501 ccgatccagc ccatcagggc cgtcccggcc agcgctccgg cgccactggc ggccgccatc
     1561 agcccgaacg ctacggcgct accgaagatc tcgctggcgt agaccgggac gatgagcgca
     1621 tcgaccggac cgatcacgag attcatggcg gcaaagaaca ggatcagtgg taagaggacc
     1681 ggctcaaagc gcacgaaacg aacgccatcc gccaactcgc gcaggtagcc acccatcgcc
     1741 cgcgatgcgt cgcgggctgt ctcgggaacg aacgcgagca tcagcagggc cgagatgccg
     1801 aacgaggccg cgtcgagcca gagcacgttg ctggcgccaa cagctgcgat gaagataccg
     1861 gtcagcgccg gtgtcgccag cccgagaacg gtcgtggcgc cctcgctgat cgcgaacgcg
     1921 cgctcggtac gcatgtcgct gagccttgcg agatccggca gcatcgccgt tcgtgccagg
     1981 ccggccggga ggtcgagcaa tgcgccggcg aagatgcagg ctagcagcag cgggaacgag
     2041 agcagcccga acgcgtgcag gaacggaatc agcagcactg acaccccgct ggccaggtcg
     2101 gcgacgacgc taacgcgccg gtagggcaag cgatcgacga gcgtcccgcc gaagaagagc
     2161 gagatgacga c
//
LOCUS       DASUGJ010000184         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_859652, whole genome shotgun sequence.
ACCESSION   DASUGJ010000184 DASUGJ010000000
VERSION     DASUGJ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859652"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..653
                     /locus_tag="VFV93_04045"
     CDS             81..653
                     /locus_tag="VFV93_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5164545.1"
                     /translation="MTTTPAPSQETIDQFVNFSHGHMAGVQEMLAADPTLINTRSSLN
                     ESPLGAAAHVGNRAMAEYLLGAGAELELPAAAMLGMEDEVRRQVAADPSLANAAGAHG
                     FPILFHAVIGGNYDLARYLVEQGADTGGDKAGAQLHAAVRTGNTDLAAWTISLGADLN
                     SPDYEGKTPLQRAEESGNAAMIALLRGQAT"
     gene            complement(667..1521)
                     /locus_tag="VFV93_04050"
     CDS             complement(667..1521)
                     /locus_tag="VFV93_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5164546.1"
                     /translation="MVSTPQTTIDGPRTHGTTRTISASRGNTFAIYLTHQITRTSRGM
                     LIWLVSLAAYGALIVAAFPSVKGTTDLESYPEALRDAFSLTTLDQIEPYLTGQILSYL
                     PLVIAFIPIMLFSGALAGAEERGSLDVLLGTPLSRRELVLTTFLTGAFNLFVVLLGLA
                     IGLWLTSLAVDAGLSIRKALAGSLVAWPIGLAFGSVALLGSAVMRQRSRVLGLAIGLM
                     FAMYALYVLSKLVDSLDWLKWLSAFHYYGAAVEDGVYWLGVAVLLATTAVLTVLAVLG
                     FERRDIYT"
     gene            complement(1524..>2310)
                     /locus_tag="VFV93_04055"
     CDS             complement(1524..>2310)
                     /locus_tag="VFV93_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5164547.1"
                     /translation="TLLGFMTPTSGSAEILDLDIVTQRDRIHEQVGNLPGEFTLDDRM
                     TGRQLIDLFGRLRNVSSSSYAFELAERLEADLDRPMRRLSRGNKQKIGIVQALFHQPD
                     VVILDEPTGGLDPLAQETFLDLLLDARARGQTIFFSSHNLTEIERVAGRVGIIRDGEL
                     VAVENPQTLTSRTFRHVQMQFADGGALATARRMIEELDGIDAVQDDGATLRFDAHGDI
                     NQVIRAVATTHVESIDIERPSLEEVFLTYYAGEADTPAGRNEA"
BASE COUNT          432 a          748 c          783 g          347 t
ORIGIN      
        1 aaggcagcat cgtcaccggc cagcgctaca tcttcgagaa ggagaacacg ggcgagtagc
       61 cgaggaaagg gcgaccgacg atgacgacga cacccgcgcc cagccaggag acgatcgacc
      121 agttcgtcaa cttctcccac ggtcacatgg ccggcgtcca ggagatgctg gccgccgatc
      181 cgacgttgat caacacccgc tcgtcgctga acgaatcacc actcggcgcg gcggcccacg
      241 tcggcaaccg ggcgatggcg gagtacctgc tcggtgccgg tgcggagctg gagctgccgg
      301 ccgccgcgat gctggggatg gaggatgagg tgcgccgcca ggtcgccgcc gatccgtcgc
      361 tggccaacgc ggccggcgcg cacggcttcc cgatcctctt ccacgccgtc atcggcggca
      421 actacgacct ggcgcgctac ctggtcgagc agggcgccga cacgggcggc gacaaggccg
      481 gcgcgcagct gcacgccgcc gtccgcaccg gcaacaccga cctggccgcc tggacgatct
      541 cgctcggcgc cgacctcaac agccccgact acgagggcaa gacgccgctc caacgggcgg
      601 aggaatccgg caacgcggcg atgatcgcgt tgttgcgggg ccaggcgacg tgagcgactg
      661 accgagctac gtgtagatgt cgcgccgttc gaagccgagg acggccaaca cggtaagaac
      721 ggcggtcgtt gccagcagga cagccacgcc cagccaatag acgccgtcct cgaccgccgc
      781 gccgtaatag tggaacgccg ataaccactt aagccaatcc aggctatcga ccagtttcga
      841 gagcacatag agagcataca ttgcgaacat caggccgatg gcgaggccca gcacccgcga
      901 gcgctgccgc atgacggctg atccgaggag ggcgacgctg ccgaatgcca gaccaatcgg
      961 ccaggcgacg aggctaccgg ccagtgcctt tctgatcgac aacccggcat cgacggccag
     1021 gctcgtcagc cagagaccga tcgcgagtcc cagcaggacg acaaacaggt tgaacgcacc
     1081 ggtcaggaag gtcgtcagca ccaactcgcg gcgggacagt ggggtgccga gcaggacatc
     1141 cagcgagccg cgctcctcgg ccccggccag cgccccggag aagagcatga tcgggatgaa
     1201 cgcgatcacc agcggcaggt agctgaggat ctggccggtc aggtatggct cgatctgatc
     1261 caacgtcgtc agcgagaagg catcgcgtaa cgcctccgga tagctctcga ggtcggtcgt
     1321 gcccttgacg gaggggaacg cagcgacgat cagcgcgcca tatgccgcca gcgagacgag
     1381 ccagatgagc atcccccggc tggtgcgggt gatctgatgc gtgagataga tcgcgaaggt
     1441 gtttccgcgg ctcgcgctga tcgtgcgggt tgtgccatgg gtacgcgggc cgtcgatcgt
     1501 ggtctgcggg gtggaaacca tcgtcacgcc tcgttccggc cagcgggggt atcagcctca
     1561 ccggcgtagt aggtcaggaa cacttcttcg agcgacgggc gctcgatatc gatgctctcc
     1621 acatgcgtcg tcgcaacggc ccggatgacc tgattgatgt cgccgtgggc gtcaaagcgc
     1681 agcgtcgcgc cgtcgtcctg gacggcatca atcccgtcca gctcctcgat catccggcgg
     1741 gccgtggcca gcgccccacc gtcggcgaac tgcatctgga cgtggcgaaa cgtccggctt
     1801 gtcagtgtct gcgggttctc gaccgcaacc agctcgccgt cgcggatgat gccaacccgg
     1861 ccggcgacgc gctcgatctc ggtcaggttg tgtgatgaga agaagatcgt ctgaccacga
     1921 gcgcgggcgt cgaggagcag atcgaggaac gtctcctgcg cgagcggatc gaggccgccg
     1981 gtcggctcgt cgaggatgac gacatccggc tggtggaaga gcgcctggac gatcccgatc
     2041 ttctgcttgt tgccccgcga gagccggcgc atcggccggt cgaggtcggc ctccagccgc
     2101 tcggccaact cgaacgcgta gctgctactg gagacgttgc ggagcctgcc gaacaggtcg
     2161 atcaactggc ggccggtcat ccggtcgtcg agcgtgaatt cgccgggcag gttgccgact
     2221 tgttcgtgaa tccggtcgcg ctgggtcacg atgtcgaggt cgaggatctc ggcgctgccg
     2281 ctggtcggtg tcatgaagcc aagcagcgtt
//
LOCUS       DASUGJ010000185         3857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1692800, whole genome shotgun sequence.
ACCESSION   DASUGJ010000185 DASUGJ010000000
VERSION     DASUGJ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692800"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..1381)
                     /locus_tag="VFV93_04060"
     CDS             complement(320..1381)
                     /locus_tag="VFV93_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5164548.1"
                     /translation="MTRQTIWKRLLMLVAILMLGSLVLAACGDDEDDADPTNTSGTAQ
                     PAATNTTGAAVESPEPSGEAEPVDYSGLSGEINIDGSSTVFPVAQAVSEEFLALAPDV
                     DFAVNASGTGGGFEKFCAGETDISDASRPIENDEIALCEQNGVDYTEFQVGVDGLSVV
                     VNIENDWIECITTDQLALIWGPDSTVKNWSDVDASWPNEPIDLYGPGTDSGTFDYFTG
                     EINGEEGASRADYTASEDDNVLVTGIAGNTYAMGYFGYAYYAENQDTLKVLGVDSGSG
                     CVTPNPETVASNEYAPLSRPLFMYIAHDSIVEKPEVAAFVEFILSDQGQAIVPEVGYV
                     ALNNEQRSAERADLADVVQ"
     gene            complement(1435..2157)
                     /locus_tag="VFV93_04065"
     CDS             complement(1435..2157)
                     /locus_tag="VFV93_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5164549.1"
                     /translation="MVRAGRQTGASSEKQSVILVVEDDEDLRRALALDLERHGYVVEQ
                     AADGETGLRIARESQIDLVVLDLMLPKLNGRHVCRLIRARDAEVPILILTALDREMDV
                     VNGLDDGADDYMTKPFSLRELLARVKALLRRSEPRAADQHVLVAGDLAIFLKEHRAVF
                     SGRELHLPLKEFRLLSALVVRKGEVCTRAELLDTVWGEDVVVDPRNVDVHIRWLRERL
                     EGDPDGSKLIQTVHGVGYRFAG"
     gene            complement(2295..3074)
                     /locus_tag="VFV93_04070"
     CDS             complement(2295..3074)
                     /locus_tag="VFV93_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164550.1"
                     /translation="MSIDDIPTWLLFVITTLLVAGTIELGYLAGKRAHRRSEDEKESP
                     VSAIAGTVLALLAFILAFSFSIVTDRYDARKALVREQAASIRTAYKSSDFLPPELRDE
                     SQELYREFIALVVTAGTTGQAGDELAETVARLRDIQEQLWDLAVENVERGDTTDISSF
                     YVQSVDEMRNVTALRVAVAVQSRMPPRLMIVLYVLVGLGMFAVGYQTAIAASRRTWMM
                     LLLALSFSIVMTLIAVLDNPERGVIGVSQQPLIDLQKQMSQ"
     gene            3438..>3857
                     /locus_tag="VFV93_04075"
     CDS             3438..>3857
                     /locus_tag="VFV93_04075"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEX5164551.1"
                     /translation="MEQFNTRQALLDTIRELRTEIDQVVASAGEDRATQPGSFEDLSL
                     KDVIAHLTGWRVVTAARLEAGLRHQEPTYPWPDHLQDDDIDTGGVDDINHWFYEQSRD
                     KSLAEVMAESNDTFDRVERAIAAMPDDDLFTPDRFAWL"
BASE COUNT          728 a         1289 c         1166 g          674 t
ORIGIN      
        1 ccggcgagac ctcggcaaag aagccggctg tctcaccgat caggacagcc acgatcgcga
       61 aggtggtcgc gaccgagagc aggccacagg cagccagcag cccgcgcacc agccgctcga
      121 cgggacggtt ctcggtcgag ccgcgcagcg ggcgggccgc catcgcggga gcagtcctcc
      181 ccctgagcga tgttgccgtc tctgccgcct tatcggccaa tccgacgtct cccttcccca
      241 cgatcatcga tgggactata caggacactg gcgatggcgg ccttctgccg ccacggccag
      301 tgtccgcgac tggtacaggc tattggacga cgtcagcgag atcggcccgc tcggcgctgc
      361 gctgctcgtt gttcagcgcg acgtagccga cctccgggac gatcgcctgg ccctggtcgg
      421 agaggatgaa ctcgacgaag gcagccactt ccggcttctc gacgatcgag tcgtgggcga
      481 tgtacatgaa cagcggccgg gagagcggcg cgtactcgtt gctggcgacc gtctccgggt
      541 tcggcgtcac gcagccggag ccgctgtcga cacccagcac cttgagcgta tcctggttct
      601 cagcgtagta ggcgtagccg aagtagccca tcgcgtatgt gttgccggcg atgccggtca
      661 cgaggacgtt gtcgtcctcc gaggccgtgt agtcggcgcg gctagcgccc tcttcgccgt
      721 tgatctcgcc ggtgaagtag tcgaacgtgc cggagtcggt gccggggcca tagaggtcga
      781 tcggctcgtt cggccaggac gcatcgacgt ccgaccagtt cttgacggta ctgtccggcc
      841 cccagatcag cgccagctga tcggtcgtga tgcactcgat ccagtcgttc tcgatgttga
      901 cgacgaccga caggccatcg acaccaacct ggaactcggt gtagtcgacg ccattctgct
      961 cgcagagtgc gatctcgtca ttctcgatcg gccgcgaggc gtcggagatg tcggtctcgc
     1021 cggcgcagaa cttctcgaag ccgccaccgg tccccgaagc gttgacggcg aaatcgacat
     1081 ccggcgcgag cgccaggaac tcctccgaaa cggcctgcgc gacggggaag acggtgctgg
     1141 agccgtcgat gttgatctcg ccggagagcc cgctgtaatc caccggctct gcctcgccgg
     1201 acggctcggg cgactcgacg gcagcgccgg tcgtgttcgt cgcggccggc tgggctgtgc
     1261 cgcttgtgtt ggtcgggtcg gcgtcatcct cgtcgtcacc acaggcagcc agcacgagcg
     1321 agccgagcat caggatcgcg accagcatca gcagtcgctt ccagatcgtc tgtcgagtca
     1381 cgtcatctcc tccacgttca acgggtccca ctcgtgacgc ccgccggcac accatcagcc
     1441 ggcgaagcga tatccaacgc catgcaccgt ctggatcaac ttcgatccat caggatctcc
     1501 ctccaaccgc tcgcgcagcc agcgaatatg gacatcgacg ttgcgcggat cgacgacaac
     1561 gtcctcgccc cagaccgtgt cgagcagctc ggcccgcgtg cagacctcgc ccttacggac
     1621 gaccagcgcc gacagcagcc ggaactcctt gagcggcagg tgcagttccc gtcccgaaaa
     1681 gacggcacga tgttccttga ggaagatcgc cagatcccca gcgacgagga catgctggtc
     1741 ggctgcgcgc ggttcggacc gccgcagcag cgccttaact cgcgccagca gctcccgcag
     1801 cgagaacggc ttggtcatgt agtcgtcggc gccgtcatcc agcccattga cgacgtccat
     1861 ctcgcggtcg agcgccgtca ggatcaagat cgggacctca gcgtcgcgcg cccggatcag
     1921 acggcaaacg tgccgaccgt tgagctttgg caacatcagg tcgagaacga cgaggtcgat
     1981 ctgcgactct ctggcgatcc gcagcccagt ctcaccatct gccgcttgct cgaccacgta
     2041 gccgtgtcgc tcgaggtcga gcgccagcgc gcgccgcaga tcctcgtcat cttccacaac
     2101 gagaatgacc gactgcttct cgctgctcgc gcccgtctgt ctacccgccc gaaccaaccg
     2161 acctgccccc tcggtaccaa ccagcacacc agttgtgcca gtcacagctc aaggctaggc
     2221 aggccgtgtt atcggcctgt gaacgaggta ttaagcttct gttagagggg ttcgagctgt
     2281 cctgctcgct gacgttactg actcatctgc ttctggagat cgatcagcgg ttgctgggat
     2341 actccgatca cgccacgctc ggggttatcc aggaccgcga tcagcgtcat cacaatcgag
     2401 aacgacagtg ccagaagcag catcatccag gttcggcgcg aggcggcaat cgcggtctgg
     2461 tagcccaccg cgaacatccc taacccgacc aggacataca gcacgatcat cagcctgggc
     2521 ggcatgcgcg attgcaccgc caccgcgacg cgcaaggccg tgacgttgcg catctcgtcg
     2581 accgactgga catagaaact ggagatgtcg gtcgtgtcgc cgcgctccac attctcgaca
     2641 gccagatccc agagttgctc ctggatgtcg cgcaacctgg ccaccgtctc agcgagctcg
     2701 tcgccggcct ggccggtcgt acctgcggta actaccagag cgatgaactc acggtacaac
     2761 tcctgggatt cgtcgcgcag ctccggaggc aagaagtcgg aactcttgta ggcggtgcga
     2821 atggacgctg cctgctcccg gaccagtgcc ttgcgagcgt cataccgatc cgtcacgatg
     2881 ctgaacgaga acgcaaggat gaaggccagc aacgccagaa ccgttccggc aatcgcggaa
     2941 accggggatt ccttctcatc ttcggaacgg cggtgggcgc gcttgcccgc gagatagcca
     3001 agctcaatcg tccccgcgac cagcagtgtc gtgatcacaa acaacagcca ggtaggaatg
     3061 tcgtcgatag acaccgatcg ctccttcacg gccgtgaggc gtcacgcgtc aggcacgcgg
     3121 gtccgctcga ccccaatcac gggcatgtct cttcagattg agccagtcta ggagtacggt
     3181 cgcgccatca gtacggctat tcggtcgcaa aatggggatg tagttgcgtc gtgattgatc
     3241 gcgctgtcgc agaaacgacg cacgacccgc ctgtcgcggc tgcgacgcgg ccatctcaat
     3301 ggacttccgc ccctcgactt ccggctcgat ccaggtgtca gggccaagat ccagggtgct
     3361 cgcatcgccc cgcgctggtg ctggattttc gggtacgatc tgcacgaacc gacgccgtat
     3421 agtggaggga tcacgcaatg gaacagttca acacccgcca ggcactgctc gacacgattc
     3481 gtgagctccg gacggagatc gaccaggtag tagccagcgc tggcgaagat cgcgcgaccc
     3541 agccaggcag cttcgaggac ctctcgttaa aggacgtcat cgcgcatctg accggctggc
     3601 gggtggtcac cgccgcgcgt cttgaagctg gcctgcgcca ccaggaacca acgtacccct
     3661 ggccagatca cctccaagac gacgacattg acactggcgg cgtggacgat atcaaccact
     3721 ggttctacga acagagtcgc gataagtcgc tcgccgaggt aatggccgag tccaacgaca
     3781 cgttcgaccg cgtcgaacgc gccatcgccg caatgcccga tgatgacctg ttcacccccg
     3841 accgcttcgc gtggctc
//
LOCUS       DASUGJ010000186         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1554972, whole genome shotgun sequence.
ACCESSION   DASUGJ010000186 DASUGJ010000000
VERSION     DASUGJ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554972"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFV93_04080"
     CDS             <1..708
                     /locus_tag="VFV93_04080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5164552.1"
                     /translation="GFAQGNNVGIAHLLKHGCDYVMLLNDDAEVAPDTIRKLVDFAES
                     DPRIGVVGPTICYYGEPRIIWSAGGAVSAHGEPSHLDVGSDLDVAGTEPRDVDYVTGC
                     AIMVKRAVIDTIGVLDDRFFIYFEETEWCARAHKAGFRVVHVPTSVMWHKVTPTARST
                     SPRYLYLMSRNRLLYLKLVGAGAGAILSAGTDMLRTSLSWLLKPQHRAMRGFSLTLVR
                     GVGAFAIGRFGPPPANL"
     gene            905..2131
                     /locus_tag="VFV93_04085"
     CDS             905..2131
                     /locus_tag="VFV93_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5164553.1"
                     /translation="MARWIGYGMALLPVVGATVPFVLGTGTSSGIVAALMLAIALLGL
                     WVLRLIATRNLVIAQSPINLPVVTVILVWLLATVSSNVFLDPRIAVRLGTAFTIVQVA
                     ALMVTMVSLGLLLLGANVGIDRRFVRLSTWAILAVGALAISAYFAGFEPQLSALNTNG
                     LFTLWVVALSYGQALFNQRLSYPARFGLLSIAGAFLVKTAIFQTGWLSGWAPAVTAVG
                     LLTLFRSRSLFFGGVVLAAIVGLGFEGRIYDAVFQSQLDEGSDSRLDIWAQAWDLLSQ
                     YPLLGTGPAGYAAYYMTLYAGSGSSLSTHSNYIDVAAQTGIAGSVAFIWLLVMVGVTA
                     WRACQRWKDGFEGGFVHATAAGFVGVIVAMALGDWLIPFVYNQGIEGFRYTVHSWVFL
                     GFMAGLANARPEQTER"
     gene            2132..>2386
                     /locus_tag="VFV93_04090"
     CDS             2132..>2386
                     /locus_tag="VFV93_04090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5164554.1"
                     /translation="MDLTVVVVSYNTRDLLERCLVTVRDQIACLRAEIVVVDNASSDG
                     SAEMVTQRFPGVTVLGNDENHGFAHAVNQGIRAGSGRYVLL"
BASE COUNT          343 a          808 c          750 g          485 t
ORIGIN      
        1 gggttcgcgc agggcaacaa cgtcggcatc gcccacctgc tcaagcatgg ctgtgactac
       61 gtcatgctcc tgaacgacga cgccgaggtc gcgccggaca caattcggaa gctcgtggac
      121 ttcgcggaga gcgacccgcg tatcggcgtg gtcggcccga cgatctgcta ctacggggag
      181 ccccgcatca tctggtcggc gggcggcgcc gtctccgcgc atggcgagcc gagccacctc
      241 gacgtcggca gcgatctgga tgtcgcggga accgagccgc gtgacgtgga ctacgtgacc
      301 ggctgcgcga tcatggtcaa gcgggccgtc atcgacacca tcggcgtcct ggacgatcgc
      361 ttcttcatct acttcgagga gacagagtgg tgcgcccgcg cccacaaggc cgggttccgc
      421 gtggtccacg tgccgacctc agtgatgtgg cacaaggtca ccccgaccgc acgaagcacg
      481 tcaccgcgct atctctatct gatgtcacgc aaccggctgc tgtacctcaa gctcgtcggc
      541 gccggcgccg gcgcgatctt gtcggccggt actgacatgc tgcggacctc gctgagctgg
      601 ctattgaagc cccagcatcg ggcgatgcgc ggattctcgc tgaccctcgt tcgcggcgtc
      661 ggcgccttcg cgatcggccg gttcgggccg ccgcccgcca atctgtagac acgtccgcca
      721 gtcctgcctg cagaatcggc cgactgcgcg acgctcttgc tctgaatcct cttggagagt
      781 ccgcatgatc ggttcgcaag ccgctccgtc cgaccatatc gcgtcatccg gcggacgcag
      841 cggcgcgcgc gagcccgcgt ggaccaaccc ggcggtgtcg ttgccgctgg agtcaacgtc
      901 ggccgtggca cggtggatcg gctacggtat ggcgctcctg ccggtggttg gcgcaaccgt
      961 tccgttcgtg cttggcaccg gcaccagcag cggtatcgtc gcggctctca tgctcgccat
     1021 tgcactactc ggactctggg ttctacgctt gattgcgact cggaatctcg tcatcgcgca
     1081 gagtccgatc aacctgccgg ttgtgaccgt catcctcgtc tggctgctgg cgacggtgtc
     1141 gtcaaacgtc ttcctcgatc cgcgcatcgc cgtacggctc ggcacggcct tcacgatcgt
     1201 tcaggttgct gccttgatgg tcacgatggt ctcgctcggg ctgctgctgc tcggcgccaa
     1261 cgtcggcata gatcgccggt tcgtccgtct ctccacgtgg gctatcctcg ccgttggcgc
     1321 cctcgctatc tcggcgtatt tcgccggttt tgagccgcag ctctcggcgc tgaacaccaa
     1381 cggccttttc accctctggg tcgttgccct gtcgtatgga caggccctct tcaatcagcg
     1441 cctgtcctat cctgcgcggt tcggcctctt gtcgatcgcc ggcgccttcc tcgtgaagac
     1501 ggccatattt cagacgggtt ggctctccgg ttgggctccg gccgtcacgg cggtgggcct
     1561 gctgaccctc tttcgctcgc gatcgctgtt cttcggcggg gtcgtgctgg cggccattgt
     1621 cggcctcggc ttcgaggggc gtatctacga tgccgtcttc cagtcacagc tcgacgaagg
     1681 ctcagactca cgcctggaca tctgggcgca agcctgggat ctcctgagcc aataccccct
     1741 gctcggaacc gggccggccg gctacgccgc ctactacatg accctctacg ccgggtcggg
     1801 ctcgtcgctc tccacgcact cgaactacat cgacgtggca gcccagaccg ggatcgccgg
     1861 ctccgtcgcg ttcatctggc tgctcgtcat ggtgggcgtg accgcctggc gagcctgtca
     1921 gcgctggaag gacgggttcg aaggcggatt cgtgcacgca acagcagccg gcttcgtggg
     1981 ggtgatcgtc gcgatggccc ttggcgactg gctcattccc tttgtctaca accagggcat
     2041 cgaggggttc cgctacaccg tgcattcgtg ggtcttcctc ggcttcatgg ccggcctcgc
     2101 gaacgcccga cccgagcaga cggagcgctg agtggatctc acagtcgtcg tcgtcagcta
     2161 caacacgcgg gatctgctgg agcgctgtct cgtcacggtt cgcgaccaga tcgcgtgcct
     2221 tcgggccgag atcgtcgtgg tcgacaacgc ctcatccgat ggcagtgcag agatggtgac
     2281 tcagcgcttc ccgggcgtca ccgtgcttgg caacgatgaa aaccacggct tcgcccatgc
     2341 ggtcaaccag ggcatccggg cgggtagcgg acgctacgtc ctgttg
//
LOCUS       DASUGJ010000187         4958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1971084, whole genome shotgun sequence.
ACCESSION   DASUGJ010000187 DASUGJ010000000
VERSION     DASUGJ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971084"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VFV93_04095"
     CDS             complement(<1..1395)
                     /locus_tag="VFV93_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX5164555.1"
                     /translation="MTPRRVLGWLLIVCCLALAACGSEEIDEPGSGDGDGSPTSAASP
                     TSEPTTTLPATPTQSATPASIAINDLKVLEWQLVQIGDEGVIDGSVVTLTMIPHTRNL
                     LNGFAECHSYFVGVEWGESSFQISESGPFAGLEQRERVCQHPEGGPEQDAAFFAALAD
                     ATSYHATDERLTLFGNDGSPRLAFVPRPPVTVDPALTGTTWQLTDLNGDSPLPGTLVT
                     LMFGESFASGNDGCNGYGAQLLTASDGRLVFGNGAEDQALCLTPEGVMEQSDRFGGAL
                     FNSVFYQVDGDQLTLTGHDGSPALTFIRQEAAAFDARLAAHRWGLIEANGQPAIPGSL
                     VTLELAKGSISGSDGCNFYGGEMIYADNGLIDVVSPGLTQTAAGCRTDDLLPQAELVT
                     GILHDASWYQIDGDRLTIGDTAGNRLVFGPQADVLLIGSSWSVPMLYTELPGGEIRGD
                     ALIEGTEITLTFTED"
     gene            complement(1480..2769)
                     /locus_tag="VFV93_04100"
     CDS             complement(1480..2769)
                     /locus_tag="VFV93_04100"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164556.1"
                     /translation="MPERGRSSKLASTLFAGLAYRDNRVFWIGTTLSSVAQASFLVSA
                     GWLAYEQGGGSATGMVAFATMFPFLLSTPIGGVLADRYDRRSIVLATQVFQGVLALLL
                     GGLAVADLLPLPLLIALVLVSSAARTVELPTVGAVLPNLVPPRVLLNNLSLNSLATLG
                     SRFAGPALLAPVLAIGGAGAAFLLIAALYLPALFWTRMTPPMPRVSVSSSGFSAQAGE
                     AIGYIRERGIVALLLVLVVLHCWLTMSFDAVLPLVAEENLGGKGAVYSSLVAAGGIGA
                     IVGSLVLAGLRVRTRRGSLLLLSGLGSGVTAILLAISGIWPVAMLAMTGVGLSQAMFM
                     TLSNTLVQEAVPDAVRGRVTSIFLMSAGGIMSFGNLANGTLADRVGVWPVLVVPGLAY
                     IVILLAVSATRPALRTVYREGILPEFASRGTPVPAGD"
     gene            complement(2784..3218)
                     /locus_tag="VFV93_04105"
     CDS             complement(2784..3218)
                     /locus_tag="VFV93_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5164557.1"
                     /translation="MPESYVIDTDVVSYLFRGDTRAEHYRALLGESVRVVSFVTLAEV
                     NYWAVSRGWGSTRRRELDDYLDQFVVVFSDGDLCEIWARVVHQARRNGRPILPSDAWI
                     AATAISLNVPLITNNLSDYAGVTELQLAHVPVVSDEDTGHHE"
     gene            complement(3211..3546)
                     /locus_tag="VFV93_04110"
     CDS             complement(3211..3546)
                     /locus_tag="VFV93_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164558.1"
                     /translation="MSIKDDLHTLVDRLDDDDAAVALSYLREILETENRVSRCGSMDE
                     QHDSLSDSEKELFADIPRRDPLALAKAQGVLPPSNFDDLLGNFWPDDESVDEFIAEIR
                     DLRRQDGYA"
     gene            complement(3624..>4958)
                     /locus_tag="VFV93_04115"
     CDS             complement(3624..>4958)
                     /locus_tag="VFV93_04115"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5164559.1"
                     /translation="HCLSVTTPPHNSLSSIADAIRAGISTTPEGEWVLVRCSFNLYAR
                     VDEMRLFTRQELDAISERHPIMVMSSLHINMLNTCALRELNLLDGEPPMGIVIHRAED
                     GTPTGVVTEVWDMVPGFTVEQVKAAIRAKGRDLFLAAGITTIHNLPYGTEDVLAVQEL
                     QAAGELPIRFRMYYHVPHQIDLDGIIAMGLKPGFGDDRLRFGGVKIFVDGVGHDGYGK
                     RVFDMKWEPAELTEFICRAHAAGQQLWMHVCSQPAIRLICDGIEEALRRHPAPHRHRI
                     EHAADYLIEDADIERVQRLGLRIITTPAFIYAQGDVLALPNSERWPRQFRLRTLVERG
                     FEVIGSSDSTGSIPDGIPPLYNIACAVTRRTLSGNRYIPEEGLDVEDALRLFTIWPAR
                     GTFEERDKGTITPGKLGDFTVISADPRTVEPDALFDLRAEATIIGGDVVWEG"
BASE COUNT          859 a         1619 c         1648 g          832 t
ORIGIN      
        1 gtcctccgtg aacgtcagcg tgatctcggt gccttcgatg agcgcgtcgc cccggatttc
       61 acctcccggc agttccgtgt agagcatcgg aacgctccag gacgagccga tcagcaggac
      121 gtcagcttgc gggccgaaga cgagccggtt gccggcggta tcgccgatgg tcagccggtc
      181 gccgtctatc tgataccagc tggcgtcgtg caggatgccg gtgacgagtt ccgcctgcgg
      241 caagagatcg tccgtccggc agccggcagc cgtctgcgtg aggcccggcg agacgacatc
      301 tatcaatccg ttatcggcgt agatcatctc gccgccgtag aagttgcagc cgtcgctgcc
      361 gctgatgctg cccttggcca gctccagcgt gacaagcgag ccggggatgg cgggctggcc
      421 gttggcctcg atcagccccc agcggtgggc ggcgagcctg gcgtcgaacg cggcggcctc
      481 ctgccggatg aacgtcagcg ccgggctacc gtcgtgtcct gtcagtgtga gctggtcgcc
      541 gtcgacctga tagaagacac tgttgaacag cgcgccgccg aagcggtcgg actgctccat
      601 gacaccctcc ggtgtcaggc ataacgcctg atcctcggcg ccgttgccga agacgagccg
      661 tccatcgctg gcggtcagca gttgcgctcc atacccgttg caaccatcgt tgccgctggc
      721 aaatgactcg ccaaacatca gcgtgaccaa cgtgccgggg agcgggctgt cgccgttgag
      781 gtctgtcagc tgccaggtcg tgccggtcag cgccggatcg accgtgacgg gtgggcgcgg
      841 gacgaaggcg agccgtgggc tgccgtcgtt gccaaagagc gtcaggcgct cgtcggttgc
      901 gtgatagctg gtggcgtctg ccagcgccgc gaagaaggcc gcgtcctgtt ccggaccgcc
      961 ctcagggtgc tggcagaccc gttcgcgctg ctcgagaccg gcgaacgggc cgctctcgct
     1021 gatctgaaag ctcgactcgc cccactcgac gccgacaaag tagctgtggc actcggcgaa
     1081 gccattgagc aggttgcgcg tgtggggaat catcgtcagc gtcacgaccg agccgtcgat
     1141 cacgccctca tcaccgatct ggaccagttg ccattcgagc accttcagat cgttgatggc
     1201 gatgctcgct ggcgtcgcgc tttgtgtcgg tgttgctggt agcgtggtgg tcggctcgct
     1261 ggtcgggctg gcagcgctcg tcgggctgcc gtcgccgtcg ccgctgcccg gctcgtcgat
     1321 ctcctccgag ccgcacgcgg ccagcgccag acagcatacg atcagtagcc aaccgagcac
     1381 gcgtcgcggt gtcacgccat cccccgtcat catgcggcca tctgtcttga tagacgctgt
     1441 gacgacggtt tcggttccag ccgaaagggc gaggtgagct tagtctccag ccggcaccgg
     1501 tgtgccgcgc gaggcgaatt caggcaggat gccttcgcgg tagacggttc gcagcgccgg
     1561 ccgggtggcg ctgaccgcca gcaggatgac gatataggca aggcccggta cgaccagcac
     1621 cggccagaca ccaacgcggt cggccagtgt gccattggcg aggttgccga acgacatgat
     1681 gccgccggcg ctcatcagga agatgctggt gacccggcca cggacggcgt ccgggacggc
     1741 ttcctggacg agcgtgttcg acagcgtcat gaacatcgcc tgtgagagac caacgccggt
     1801 catcgccagc atcgccaccg gccagatacc ggagatcgcc agcaggatgg ccgtgacacc
     1861 gctccccagc ccactcaaca gcagcagcga gccccgccgc gtccgcaccc gcaagccggc
     1921 cagcacgagc gagccgacga tcgcgccgat cccgccggcc gcgaccagcg acgagtagac
     1981 ggcgcccttg ccgcccaggt tctcctccgc caccagcggc aggacggcgt cgaacgacat
     2041 cgtcagccag cagtggagga cgaccagcac cagcagcaac gcgacgatgc cccgctcgcg
     2101 gatgtagccg atcgcctcgc cggcctgggc gctgaagccg ctgctgctca ccgaaacgcg
     2161 cggcatcggc ggcgtcatcc gcgtccagaa gagcgcaggc aggtagagcg ccgcgatcag
     2221 caggaaggcc gcaccggccc cgccgatcgc cagcaccggc gccagcaggg ccggccctgc
     2281 gaagcgcgag ccaagcgtcg ccagtgagtt gagcgagagg ttgttgagca ggactcgcgg
     2341 cgggacgaga ttcggcagga cggccccgac cgtcggcagc tcgaccgtcc gcgccgcgct
     2401 ggagacgagg accagcgcga tcagcagcgg cagcggcagc aggtcggcga cggccagccc
     2461 acccagcagc agcgccagca cgccttggaa cacctgcgtc gccagcacga tcgagcggcg
     2521 gtcgtagcgg tcggccagga ccccgccgat tggcgtcgag agcaggaagg ggaacatcgt
     2581 cgcgaaggcg accatcccgg tcgcgctgcc gccaccctgc tcgtaagcca gccagccggc
     2641 cgagaccaga aacgaggcct gcgccacgct ggagagcgtc gtcccgatcc agaagacccg
     2701 gttatcccgg taggccagcc ccgcaaacaa cgtgctggcc agcttgctgc tgcgcccccg
     2761 ttcaggcatt gagatcgccg acgctactca tggtgtccag tatcctcgtc gctaacaacc
     2821 ggtacatgcg ccaactgaag ttcggtaact cctgcatagt cgctcagatt atttgtgatg
     2881 agtggaacat tcagactgat cgccgttgct gcgatccatg cgtccgacgg gaggatcggt
     2941 cgtccattcc gtcgtgcctg gtgaacgacc cgagcccaga tctcgcagag gtcgccatca
     3001 gagaagacga cgacaaactg atccagatag tcgtcgagtt cgcgtcggcg agtgctgccc
     3061 caccctcggc taacggccca gtagttcact tccgcgagcg tcacgaaaga cacgactctg
     3121 accgactcac ccagaagcgc gcggtagtgt tctgccctgg tatcgccgcg aaacaggtac
     3181 gacacaacat ccgtgtcgat gacgtagctc tcaggcatag ccgtcctgac gtcgcaagtc
     3241 tcgaatctca gcgatgaact catccacgga ttcgtcgtca ggccagaagt taccaagcag
     3301 atcgtcgaag ttgctgggcg gtagtacccc ttgcgccttc gcaagtgcaa ggggatcccg
     3361 gcgaggaata tctgcgaaga gttccttctc tgaatcgctc agcgaatcgt gttgctcatc
     3421 cattgatccg catcgggaca ccctgttctc ggtctcgaga atttctcgca agtacgagag
     3481 cgcaactgcc gcatcgtcat catcgagtcg gtcgacgagt gtgtgaagat catccttgat
     3541 gctcatggcg cgctgccctc catttcgttc actccattgt tggcattcgc gacatgcatg
     3601 ggattgtacg atagctggat tgcctatcct tcccagacaa cgtcgccgcc gatgatggtg
     3661 gcttcggcgc gcaggtcgaa gagtgcgtcg ggctcgacgg tgcgcgggtc ggcggagatg
     3721 acggtgaagt cgccgagctt gccgggggtg atcgtgccct tgtcgcgctc ctcgaacgtg
     3781 cctcgggccg gccagatcgt gaagagccgc agggcgtcct cgacatccag cccctcttcg
     3841 gggatgtagc ggttgccgga gagggttcgg cgggtgacgg cgcaggcgat gttgtagagc
     3901 ggcgggatgc cgtcggggat cgagccggtc gagtcgctcg atccgatgac ctcgaagccg
     3961 cgctcgacca gcgtccgcag ccggaactgg cgcggccagc gctccgagtt cggcagcgcg
     4021 aggacatcgc cctgggcgta gatgaaggcc ggcgtcgtga tgatgcgcag gccgagccgc
     4081 tggacgcgct cgatatcggc gtcctcgatc agatagtcgg cggcgtgctc gatccggtgc
     4141 cggtgcgggg ccgggtggcg gcgcagcgct tcctcgatgc cgtcgcagat cagccgaatg
     4201 gccggttgcg agcagacgtg catccagagc tgctggccgg cggcgtgcgc gcggcagatg
     4261 aactcggtca gctcagccgg ctcccacttc atgtcgaaga cgcgcttgcc gtagccgtcg
     4321 tggccgacgc cgtcgacgaa gatcttgacg ccgccaaagc gcagccggtc gtcgccgaag
     4381 ccgggcttga gccccatcgc gatgatcccg tcgaggtcga tctggtgcgg gacgtggtag
     4441 tacatccgga agcggatcgg cagctccccg gccgcctgta gctcctgaac ggccagcacg
     4501 tcctcggtcc cgtagggcag gttatggatc gtcgtgatcc ccgccgccag gaacaggtcg
     4561 cgtcccttgg cgcggatggc cgccttgacc tgctcgacgg tgaagcccgg gaccatgtcc
     4621 cagacctcgg tgacgacgcc ggtcggtgtg ccgtcctcgg cgcggtggat gacgatcccc
     4681 atcggcggct cgccgtccag caggttcagc tcccgcagcg cgcaggtgtt gagcatgttg
     4741 atgtgcagtg acgacatgac catgatcggg tgccgctcgg agatcgcgtc cagctcctgg
     4801 cgggtgaaga gccgcatctc gtcaacccgc gcatacaggt tgaacgagca gcggacgagg
     4861 acccactcgc cctccggtgt cgtgctgatc cctgcgcgga tcgcatcggc gatgctgctg
     4921 aggctgttgt gcggcggcgt cgtgaccgag aggcaatg
//
LOCUS       DASUGJ010000188         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1414617, whole genome shotgun sequence.
ACCESSION   DASUGJ010000188 DASUGJ010000000
VERSION     DASUGJ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414617"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1894
                     /locus_tag="VFV93_04120"
     CDS             116..1894
                     /locus_tag="VFV93_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5164560.1"
                     /translation="MNPAWNDAELEQQVLDTISLDEPWTLIEEFSTLVRLSGSAEEAE
                     AVKQITDRLDRWGIDYEVHHPTCLISLPGPATLRTVGDGGRSYTVKTPSFSPHTNGRE
                     VEAELVYIPGHQAAGINELFSEQRTAADVDLRGKIVMTEGLGIAARGFDLAASGAVAA
                     LFINPGNRIHEGITTTTWGSPDLDSLGRVPPVPILTINNPDGKELIAQLQHGPVKLAF
                     SNRTDTGWRPIPVVVSEIRGNRFPDEFVLFHGHLDSWHIGIGDNATGDATLLELARVF
                     KQHQGSLDRTLRVAWWSGHSHGRYAGSTWYADEYALDLARNCVAHVNVDSPGCRWATV
                     YENVAWMSETESFCQSVIRDITGQDSSGEGHVLRAGDCSFNNQGVTTCSMLSSTMPQD
                     LVAEKGYYPVGGCGGNIAWHTEDDTMEIADRDNLLRDMRVYAATLLRILNAPIAPFDY
                     RATLTEIDDAVQRYSASAQSVIDLAPTREAVARLRNALDAFYEQTAGLNARDDAALRA
                     ANATQRAVARVLVNLCYTQDGRFKQDPARAIPAVPDLASALQLPDADDDMRRIITNQL
                     RRGQNHLIWELDGARATIDRAMAARS"
BASE COUNT          386 a          758 c          585 g          324 t
ORIGIN      
        1 gagatccttc gcttgcgctc aggatgacag aggtgagacc ctatgccccg aatctgaccg
       61 acgcccgtac aatgccctcc aactgaccga cacagcacag ggagggaacc agcacatgaa
      121 cccagcctgg aacgacgccg aactcgaaca acaggtcctc gataccatct ccctcgacga
      181 gccatggacg ttgatcgagg agttctcgac actcgttcgt ctgtccgggt cagcggaaga
      241 ggcggaggcg gtcaagcaga tcaccgaccg gctggaccgc tggggcatcg actacgaagt
      301 ccaccaccca acctgcctga tcagcctgcc cggtcccgcc acgctccgaa ccgtcggtga
      361 cggcggcagg agctacaccg tcaagacgcc gtcgttctcg ccgcatacca atggtcggga
      421 ggtcgaagcc gaactcgttt acattcccgg ccaccaggcc gccggcatca acgagctgtt
      481 cagcgagcag cgcacggccg ccgatgtcga cctgcgcggc aagatcgtca tgaccgaggg
      541 gctgggcatc gccgcccgtg gcttcgacct ggctgcctcc ggcgcggtcg ccgccctctt
      601 catcaacccc ggcaaccgca tccacgaggg catcacgacc accacctggg gctcgcccga
      661 tctcgactcg ctcggccgcg tcccgccggt cccgatcctc acgatcaaca atccggacgg
      721 caaggagctg atcgcacagc tccagcacgg cccggtcaag cttgccttct cgaacaggac
      781 cgacaccggc tggcggccga tcccggtcgt cgtctcagag atcaggggta accgcttccc
      841 ggacgagttc gtcctctttc acggccacct cgactcctgg cacatcggca tcggcgacaa
      901 tgccaccggc gacgcgaccc tgctggaact cgcccgcgtc ttcaagcagc accagggttc
      961 gctcgaccgc acgctgcggg ttgcctggtg gtccggccac tcgcatggcc gttacgccgg
     1021 ctcgacctgg tacgccgacg agtacgccct cgacctggcc cgcaactgcg tcgcccacgt
     1081 caacgtcgat tcccccggtt gtcgctgggc aaccgtctac gagaatgtcg cctggatgag
     1141 cgagaccgag tcgttctgcc agtccgtcat tcgcgacatt accggccagg attccagcgg
     1201 cgagggtcac gttctgcgcg ccggcgactg ctcgttcaac aaccagggtg tcacgacctg
     1261 ctccatgctc tcatcgacga tgccgcagga cctggttgcc gagaagggtt actacccggt
     1321 cggcggctgc ggcggcaaca tcgcctggca caccgaggac gacacgatgg agatcgccga
     1381 tcgcgacaac ctgctgcgcg acatgcgcgt ttacgccgcg acgctgctgc gtatcctcaa
     1441 cgcgccaatc gccccgttcg actaccgcgc caccttgacc gagatcgacg acgccgtcca
     1501 gcgctacagc gcctccgccc agtcagtcat cgacctcgcg ccaactcgtg aagccgttgc
     1561 gcggctccgc aacgcgcttg acgcgttcta cgagcagaca gccggcctca atgcccgcga
     1621 cgatgcggcc ctgcgggctg ccaacgccac ccagcgggcc gtcgccagag tcctcgtcaa
     1681 tctctgctac acccaggacg gtcgcttcaa gcaggaccct gcccgcgcca tccccgccgt
     1741 cccggacctc gccagcgcgc tccagcttcc ggacgccgac gacgacatgc gacgtatcat
     1801 caccaaccag ctccgtcgtg gtcagaatca tctaatctgg gaactcgacg gcgcccgtgc
     1861 caccatcgat cgggcgatgg cggcacgaag ctaatggaat gacagcgtgc tggcggtcaa
     1921 gggagtccgg ccagaccgcc agcactcaaa tcaacgctta acgtttatta cggatccgtc
     1981 gcccccatcg ccagcaacgc gtcgcggcag ccgcgcaggt actccaggtg caacccggcg
     2041 atcacgacat cct
//
LOCUS       DASUGJ010000189         9207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_998897, whole genome shotgun sequence.
ACCESSION   DASUGJ010000189 DASUGJ010000000
VERSION     DASUGJ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998897"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFV93_04125"
     CDS             complement(<1..635)
                     /locus_tag="VFV93_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164561.1"
                     /translation="MSEYQYYEFQAIDNPLTPQRMVELRALSTRAAITPTRFQNVYHY
                     GDFKGDPLAVMERYFDAHVYVANWGTHQFMLRPPARLLDMQTAQAYAIGGSLDVYSRG
                     DIVILEFLSTDEDGGGWIDDNESASWLTALLPLRSELAGGDLRALYLGWLAGAEAGML
                     DDDETEQPVPPGLNQLSAALKSLAEFLSISEDTLAIAATRSSKLSDAPSSN"
     gene            complement(807..878)
                     /locus_tag="VFV93_04130"
     tRNA            complement(807..878)
                     /locus_tag="VFV93_04130"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(844..846),aa:Asn,seq:gtt)
     gene            1036..2022
                     /locus_tag="VFV93_04135"
     CDS             1036..2022
                     /locus_tag="VFV93_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5164562.1"
                     /translation="MASSDGQRSDSALAIAVEIGGRGHRVALADADGRIIDSAHAIGH
                     GANAAVTVDTICGLIESIMSRRNVDDDVVQGIGVAFGGPVDANRGITQRSHRVPGFEH
                     FPLAGVIEDRFGIRTVLENDARAAALGEFTLGAGRGARDMVYLHLGFGVGGAIVLDGR
                     LQHGASMTAGEIGHMVVSANGHDGPRCSCGKPGHLEAFASEPSIIERMRLRLIAAEPE
                     VKAEWLASPGITVRRIFDSVETDEHAEAIVNETVQVIGLALANLVTALNPDAVVIGGY
                     VAETGPAMTAAIRAKIRQYAFDAAARRVTVAAAQLGADAGLVGAVVLALRPS"
     gene            2292..4049
                     /locus_tag="VFV93_04140"
     CDS             2292..4049
                     /locus_tag="VFV93_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164563.1"
                     /translation="MQTEQTAPTLDLEGNAKQKELAGRLFRVFEAQGRFFSSNAPIRI
                     TLAQLAEFMGQQESGKSDWTKDINAALSASKKVFAREEQDDEVVFVTTRSGRVPVDMV
                     DIDQAHSLGKRFATPEPKRAIPPTRRLKPSRPSPYYIESSYPTTEPSISRSFAPPADD
                     TYVVVPTTLPTHVEPLAEVEDELIPAPPVIEEDIEELAPAPVAETVSGEAIEIASASD
                     EAIVDAIHQSLGQEMSVARWGDLWMMEDKVQRFSRGDLRRIEDFLREQGGIANDEEIV
                     QDILGVRTNADEYAGTRFALNYRLSRETREFEYVGTDTAGVWSLSNQPSIGTTKRKPS
                     EIGQDYRFLIDYRTPDEGLEEGIIEHILSFYEYVYGVLPLDANLATLLPRAGFADQRA
                     ARVTFESPQTGETIVTELRFPTSNRGGFVAGLEQFFADNLVPGAVLTLERTERPNHFL
                     LEYFQVSGEDRKLLHLDERKGKYAFRSMTYYCATQDDFLLTEGRFPKLADVKPLDDRA
                     RRRPEQVVQVTFERVGENTGSADAPSYSASFSDLLAAANIERPISAEYLRDILTSGTY
                     PEFSADESGEDVYIFQPVS"
     gene            4021..4974
                     /locus_tag="VFV93_04145"
     CDS             4021..4974
                     /locus_tag="VFV93_04145"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5164564.1"
                     /translation="MSTSSSQFPEAGSRAAVERVLADWLASTETTTSVEGISDLSGAG
                     AEYLAERWPEIPVEARRSLVRAMTADAEEQIEHNFNRALLIAIRDQDAQIRLEALEGL
                     AELESQAFLETLLSVIDREPDARVRTAEAMALGRFALQCELETLEPELANRVRTTLLH
                     LIESDESSDVRRRALEAAGYFAEDADVIREIEVAYESGNHALRVSALHAMGRQADTRW
                     LEIVHEELTNDDPELRYEAVVATGSIGDERSVVELIDRLRDEDVEVQLAAIASLGEIG
                     GRLAISALRRLTEDDSPAIVDAAEAALEEAQLTSDPLRPLM"
     gene            5035..6582
                     /locus_tag="VFV93_04150"
     CDS             5035..6582
                     /locus_tag="VFV93_04150"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX5164565.1"
                     /translation="MRLSDLLTGIDACDVTGDATVDIDQIEFDSRLVTPGSLFVALRG
                     GYTDGHLFLQQARDRGAIAAVVEIDATDAEMGGFRAVVRATDNRALLARLATRFYAAP
                     SQAMTVVGVTGTDGKTTTSYFIEAMCRHAGRRTGMIGTVAVRIGADIDLHESRQTTPE
                     SLHIQHYLAEMRDHGVDLAVVEATSHGLELHRLDGCDFDIGVITNVTHEHLDFHGSIE
                     NYRRAKGGLLRRVASARQLGKLGIAVINADDEGARSIEPDAAGCTILRYSTAGAAVAD
                     VQAEQIETDPAGATFVLRTPAGSHPAHIHLPGAYNIANAMAAVSVGVALGIPIETIVG
                     GIASLRAVPGRMETVSAGQPFAVIVDYAHTPDAIRSVLREARKLATGQVLVVFGSAGE
                     RDVAKRAVQGAVAVTDADFAVFTSEDPRFEDPDAIVDEIAAGAVEAGGRRGVDFDCIE
                     DRRQAIAAALDRAGAGDVVVLAGKGHEQSMIYGSEKRPWNEAAVAREILRQLGYQRND
                     DSGGSER"
     gene            6579..7640
                     /locus_tag="VFV93_04155"
     CDS             6579..7640
                     /locus_tag="VFV93_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164566.1"
                     /translation="MTVARRSRTMPVLIVTLMIAASIVLSGCGQPDADLTLNTPVSQT
                     GADPSGRILFVADGQVQMWDGGDIKQITDVGDEMEALSPTWAPDGNRFAYVQANRSKG
                     YSDLYVANLDGETLKQVTNNAPDVDPFSQEFVCNAYWVADPVWDQAGERIIWASDRGG
                     WEFDQCENRLSDPMFLWYSETWDADPYILPATADIGLAQEGPTLSGNGQMAAFVVREE
                     VTDSLHNPQIWTLDMNTAETTILIDHPDGAYDPAWSPDDHNVAYIQRDGNRNDVWIAP
                     LDGSAPYRLTDIGTCVSPTWSPDGRYVAFFRETDGNFEAAYVEVDAGADGKLSASDPQ
                     KLFSAENISTISGMSWSAR"
     gene            complement(7741..>9207)
                     /gene="aspS"
                     /locus_tag="VFV93_04160"
     CDS             complement(7741..>9207)
                     /gene="aspS"
                     /locus_tag="VFV93_04160"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX5164567.1"
                     /translation="TPFEISDDTNVDESLRLEYRYLDLRRPRMQRNLMLRHQIVRTIR
                     RYLDERDFIEIETPILIKSTPEGARDYLVPSRIYPGQFYALPQSPQQMKQILMVAGMD
                     RYYQIARCFRDEDQRADRQPEFTQLDLEMSFIDMEDVIQLTEGLFIELVGLTDFTIQE
                     LPFPRLTYADAMLRYGSDKPDLRYGLEIADVSSLVAGSEFGVFANAVAGGGVVRGIAV
                     PGRGGSSRGQIDELTNFAKQFGARGLAWLAIEEGDDGARSARSPIAKFLSQAELDGIA
                     DATGANTGDLVLLVADSADVAANVLGRLRERFGNELGLADPNVAAFCWVIDFPLFGWD
                     AENQRWDSLHHPFTAPMDEDIALLDSDVGAVRSKAYDIVLNGFEVGGGSIRIHDQQLQ
                     SRMFQLMGYEQSEIDERFGHLLRAFEFGAPPHGGIAPGIDRIAMILAREETLREVMAF
                     PKNQSARDLMFDAPGDVEPKQLDELHIRVNEPVAAKGN"
BASE COUNT         1697 a         2899 c         3006 g         1605 t
ORIGIN      
        1 tcgttcgacg acggcgcgtc tgaaagcttg ctgctgcgcg tggcggcgat cgcgagggtg
       61 tcctcgctga tgcttaggaa ttcggcgagc gatttcagtg ccgccgacag ctggttgagt
      121 cccggcggga ccggctgttc cgtctcatcg tcatcgagca tgcctgcctc agcgccggcc
      181 agccagccga ggtagagggc acgcagatcg ccacctgcga gttcagatcg cagcggcagg
      241 agtgccgtca gccagctcgc ggattcgttg tcgtcgatcc agccgccccc atcttcgtca
      301 gtggagagga actcgagaat cacgatgtca ccacggctgt agacgtccag cgagccaccg
      361 atcgcgtacg cctgagcggt ctgcatgtcc agcaggcgtg ctggcgggcg cagcatgaac
      421 tggtgcgttc cccagttcgc gacgtagacg tgggcgtcga agtagcgctc catcaccgcc
      481 agcggatcgc ccttgaagtc gccatagtgg tagacgttct gaaagcgcgt cggcgtgatg
      541 gcggcgcggg tcgagagtgc acgcaactca accatccgct gcggcgtgag cggattgtcg
      601 atcgcctgga actcgtagta ctggtactcg ctcatcgttg ctacctcgcc acttctcaga
      661 acggtaacgt gaacgagtcg gcgcaacctg gtgttcggtc aatggagtcg ttcgccccgg
      721 aacccagtct gagtcaagga tacggcgcga aagcgagaca ccgcccggtt tagccgggcg
      781 gtgtctcgcg ctgctgaact ttcgggctcc ccgacatgga ctcgaaccat gaaccttacg
      841 gttaacagcc gtctgctcta ccattgagct atcggggatc gatatcgtgc tcaagcgacg
      901 aaaattctag catacccagc caaagcccgg caagcacagg cgagattgtg aagtatggta
      961 caatgtgccc gcttcccgcc aaagcgactc ctctcgcggt caactggtac agggcaggat
     1021 aaggggcaat cggtcgtggc gtcatcggac ggtcaacggt ccgactcagc gttagccatc
     1081 gccgtcgaga ttggcggccg gggccatcgt gtcgcgcttg ccgacgcgga cgggcgcatc
     1141 atcgacagcg cccacgcgat cggccacggc gccaacgcgg ccgtcacggt tgacacgatc
     1201 tgcgggctga tcgagtcgat catgagccgg cgcaatgtcg acgacgatgt cgtccagggg
     1261 atcggcgtcg ccttcggtgg gccggtcgac gcgaatcgtg gcatcaccca gcgctcccat
     1321 cgcgtgccag gcttcgagca tttcccgctg gcaggcgtca tcgaggaccg tttcggcatt
     1381 cgaaccgtgt tggagaacga cgctcgtgcc gccgcgctgg gtgagttcac gctcggcgcc
     1441 ggacgtggcg cgcgggacat ggtctacctg cacctgggtt tcggcgttgg cggcgcgatc
     1501 gttctcgacg gccgcttgca gcacggggcg tcgatgacgg ctggtgagat aggccacatg
     1561 gtcgtgtcgg ccaacggtca cgacgggccg cgctgctcgt gcgggaagcc cggtcatctg
     1621 gaagcgttcg cctccgagcc gtcaatcatc gagcgaatgc gcctgcgcct gatcgcggcc
     1681 gagccggagg tcaaggccga atggctggcg agtcccggca tcaccgttcg gcgcatcttc
     1741 gacagcgtcg agaccgacga acacgccgag gcgatcgtca acgagaccgt ccaggtgatc
     1801 gggttggcgc tggccaacct ggtgacggcg ctgaacccgg atgctgtcgt gatcggcggc
     1861 tacgtcgccg agacgggtcc ggcgatgacg gcggcgatcc gcgccaagat ccggcagtac
     1921 gccttcgacg ccgctgcccg gcgggtgacc gtcgcggcgg cgcaactggg cgctgatgcc
     1981 ggcctggtcg gcgctgtcgt gctcgcgctt cgcccgtcct gatcagccgg cgacgatgta
     2041 cacttacggg ccgcgatatg cgtgagatgg agagtagcaa ccatgacatt gagtgattcg
     2101 gacgtgatgt atctgttgac gttgcttcgc tccagtgagc aaccgatgtc gaccgagcaa
     2161 ctggtacagg cgctcaaaca gcgcgcaaag gcataacccg acggccactc aggccagcac
     2221 gaccactgag gacaggtacg aaccagcgtg gtcgcagacg acgtagcagc gcgagagagg
     2281 gatatcgctc catgcaaacc gagcagacgg ctccaacgct tgaccttgag ggcaacgcaa
     2341 aacagaagga gctggccggc cgcctgttcc gggtatttga ggcacagggc cgatttttca
     2401 gcagcaacgc gccgatccgc atcacactgg cacaactggc cgagttcatg gggcagcagg
     2461 agtccggtaa atccgactgg acgaaggaca tcaacgccgc cttgtcagcc agcaagaagg
     2521 tctttgcgcg cgaggaacag gatgacgaag tcgtcttcgt cacgacgcgc tccggtcgcg
     2581 ttccggttga catggtcgat atcgatcagg cgcactcgct cgggaagcgg tttgccacgc
     2641 cggagccgaa gcgcgcgatc ccgccgactc gccggctgaa gccgagccgc ccgtcgccgt
     2701 actacatcga gtcgtcgtat ccaacaaccg aaccttccat ctcccgctcg ttcgcgccac
     2761 cggcggacga cacctatgtt gtcgtgccaa ccacgctgcc aacccacgtc gaaccgctgg
     2821 cagaggttga agacgagctg atcccggcgc ctccggtgat tgaagaggac atcgaagaac
     2881 tggcaccagc gcccgttgct gagaccgtga gcggtgaagc gattgagatc gcatcggcca
     2941 gcgatgaggc catcgtcgac gctatccacc agtcgctcgg tcaggagatg agcgtcgcgc
     3001 gctggggtga cctctggatg atggaggata aggtccagcg gttctcgcgt ggtgacctgc
     3061 gtcgaatcga ggacttcctg cgcgagcagg gcggtatcgc caacgacgag gagatcgtcc
     3121 aggacatcct cggcgtccgg accaacgccg atgagtacgc cggcacgcgc ttcgccctga
     3181 actaccggct gtcccgcgag acgcgcgagt tcgagtacgt tgggacggac acggccggcg
     3241 tctggtcgct gtccaaccag ccgtcgatcg ggacgaccaa gcgcaagccg agcgagatcg
     3301 gccaggacta tcgcttcctg atcgactacc gcacgccgga cgaaggtctc gaagagggca
     3361 tcatcgagca tatcctgagc ttctacgagt atgtctacgg cgtgttgccg cttgacgcta
     3421 acctggcgac gttgctgccg cgtgccggat tcgccgacca gcgagcggcg cgggtgacct
     3481 ttgagtctcc gcagaccggc gagacgatcg tgaccgagtt gcgcttcccg accagcaatc
     3541 gcggcggttt cgttgctggg ctggagcagt tcttcgctga caatctggtg ccgggcgccg
     3601 tcctgacatt ggagcgcacc gagcgcccga accacttcct gctggagtac ttccaggtct
     3661 ccggcgagga tcgcaagctg ctgcatctgg acgagcgcaa gggcaagtac gccttccgct
     3721 cgatgacgta ttactgcgcg acccaggacg acttcctctt gacggagggc cgcttcccga
     3781 agctcgccga tgtcaagccg ctcgatgacc gcgcacgccg ccggccggag caggttgtcc
     3841 aggtgacgtt cgagcgcgta ggcgagaata ccggatcagc ggacgcgccg agctactcgg
     3901 ccagcttcag cgatctgctg gcggccgcga acatcgaacg gccgatctcg gcggagtacc
     3961 tgcgcgatat tctgacgagt ggtacctatc ccgagttcag tgcagacgaa tcaggtgagg
     4021 atgtctacat cttccagcca gtttcctgag gccggtagtc gggcggccgt cgaacgcgtc
     4081 ctcgcggact ggctcgcctc aaccgagact acgacatccg tcgaggggat ctccgatctg
     4141 agcggggcag gggccgagta tctggccgaa cgctggccgg agataccagt tgaagcccgg
     4201 cggtcactcg tcagggcgat gactgcggac gccgaggagc agatcgagca caacttcaat
     4261 cgtgcgctcc tgatcgcgat ccgcgatcag gacgcgcaga ttcgcctcga agcgctcgag
     4321 gggctggccg agctagaatc gcaggcgttc ctggagacgc tgttgagcgt catagatcgc
     4381 gagccggatg cgcgagtgcg gacagctgag gcgatggcgc ttggtcgctt cgcgctgcaa
     4441 tgcgagctcg aaacgttgga acccgaactg gcgaaccgcg tcaggacgac gctgctgcac
     4501 ctgatcgaga gcgacgagtc atcagatgtc cgacggcggg cgctcgaggc tgcaggctac
     4561 ttcgcggaag atgccgatgt catccgtgag atcgaggttg cctacgaatc ggggaaccat
     4621 gccctgcgcg tcagcgcgct gcatgcgatg gggcggcagg ctgacacccg ctggctcgaa
     4681 atcgtgcacg aggagctcac caacgacgac cccgagttgc gctatgaagc agtcgtcgcg
     4741 actggttcga tcggcgacga gcgctcggtc gtcgaactca tcgaccggct gcgcgacgag
     4801 gatgtggaag tgcaactggc cgcgattgcc tcgcttggag agatcggcgg ccggttggcc
     4861 atcagcgcgc tgcgccggct gaccgaggac gactcgccgg ccatcgttga cgcggccgaa
     4921 gcggccctcg aagaggcgca actcacgtca gacccgctac gcccgttgat gtgatggccg
     4981 aaatcgccat tctccgaatt gcctcgcagt tcccgacgac ggaaaggctc gccagtgcgc
     5041 ctgagtgacc tgctgactgg tattgacgcc tgtgatgtta ccggcgacgc caccgtcgat
     5101 atcgaccaga tcgagtttga cagccggctc gtgacgccgg gcagcctctt cgtcgcgcta
     5161 cggggcggct acacagatgg ccatctcttc cttcagcagg cgcgcgatcg tggcgcgatc
     5221 gcggcagttg tagagatcga cgcgaccgat gccgagatgg gtggatttcg cgctgtcgtg
     5281 cgggctacgg acaaccgcgc gctgctggcg cgacttgcga cacgcttcta cgccgcaccc
     5341 agccaggcga tgaccgtcgt cggcgtgacc ggcaccgacg gcaagacgac gaccagctac
     5401 ttcatcgaag cgatgtgtcg ccatgcgggc cggcgaaccg gcatgatcgg tactgtcgcg
     5461 gtccggatcg gcgccgacat tgacctgcac gagtcgcggc agacgacgcc cgagtcactg
     5521 cacatccagc actacctggc cgagatgcgc gatcacggcg tcgacctggc cgttgtcgaa
     5581 gcgacgtcgc atggccttga gctccaccgg ctggacggct gcgatttcga tatcggtgtg
     5641 attaccaacg tcactcacga gcacctcgat ttccacggct cgatcgagaa ctaccgccga
     5701 gcaaaaggcg ggctgctccg tcgggttgcg tccgccaggc aacttggcaa gctcggtatc
     5761 gccgtcatca acgccgacga cgaaggcgcg cgatcaatcg aaccggatgc cgccggctgc
     5821 acgatcctgc gttactcgac agctggcgcg gccgtagcag atgtgcaggc cgagcagatc
     5881 gagaccgatc cggccggcgc gacgttcgtt cttcggacgc cagcgggcag ccatccggcc
     5941 catatccacc tgccgggtgc gtacaacatc gcgaacgcga tggcggcggt gtcggttggc
     6001 gttgcgctgg gcattccgat cgagacgatc gtaggcggca tcgcgtcact cagggccgtt
     6061 ccgggtcgga tggagacggt cagcgcagga cagccgtttg ctgtcatcgt cgactacgcc
     6121 cacacaccgg acgcgatccg cagcgtcctg cgggaggcac gcaagctggc gaccgggcag
     6181 gtgctggtcg ttttcggctc ggcgggagag cgcgacgtcg ccaagcgggc cgtccaggga
     6241 gcggttgccg taaccgatgc cgactttgcg gtctttacca gcgaagaccc gcgcttcgag
     6301 gaccccgacg cgatcgtgga cgagatcgct gccggtgcgg tcgaggccgg cgggcggcgc
     6361 ggtgtagact ttgactgtat cgaagatcgt cgacaggcca tcgcggcggc gctggaccga
     6421 gccggagcag gcgatgtcgt tgtgctggcc gggaagggtc atgaacagag catgatctac
     6481 ggatccgaga agcgtccgtg gaacgaagcc gctgtcgcac gcgagatcct gcggcagttg
     6541 ggctatcaga gaaacgacga ctccggaggt tcggaacgat gaccgtggcg cgacgctccc
     6601 gaacgatgcc ggtactgatc gtgacactca tgatcgccgc gtcgatcgtc ctcagtggct
     6661 gcggtcagcc cgacgctgac ctgaccctca ataccccggt ctcgcagaca ggcgccgacc
     6721 catctggtcg catcctcttc gttgcggacg gccaggtgca gatgtgggac ggcggcgaca
     6781 tcaagcagat caccgatgtc ggcgacgaga tggaagcgct ctcgccaacc tgggctcccg
     6841 acggcaatcg gttcgcctat gtccaggcaa accgcagcaa gggctattcg gacctctacg
     6901 tcgccaatct tgatggtgag acgttgaagc aagtgacgaa caacgccccc gatgtcgatc
     6961 cgttttcgca agagttcgtc tgcaacgcct actgggtggc cgacccggtc tgggaccagg
     7021 ccggcgagag gatcatctgg gccagcgatc gcggcggctg ggagtttgac cagtgcgaga
     7081 accggctctc cgacccgatg ttcctctggt attcggaaac gtgggacgcc gatccgtata
     7141 tcctgccggc gacggccgat atcggcctcg cccaggaagg cccgacactc tccggcaacg
     7201 gccagatggc cgccttcgtc gtccgtgaag aggtgaccga ctcactgcac aacccgcaga
     7261 tctggacgct cgacatgaac accgctgaga cgacgatcct gatcgatcac cctgacggcg
     7321 cctacgaccc ggcctggtcg cctgacgatc acaacgttgc ctacatccag cgcgatggca
     7381 accgcaacga cgtctggatc gcgccgctgg acggtagcgc cccgtatcgc ctgaccgata
     7441 tcggcacctg tgtctcgccg acctggtcgc ccgatggtcg ctacgtcgcc ttcttccgcg
     7501 aaaccgacgg caacttcgag gctgcctatg tcgaggtcga cgcaggcgcc gacggcaagc
     7561 tcagcgcctc cgacccgcag aagctcttct ccgccgaaaa catcagtacc atttcaggca
     7621 tgtcctggtc agccaggtaa tcccgcgtag gggtggacgc agcctcgccc ttcttaggcc
     7681 gcgcacgggc tggaaaggga gagtccgcgg ccatcgcccc tacgcggttg tgatcctacg
     7741 tcagttgcct ttggcggcta ctggttcgtt cacgcggatg tgaagttcgt cgagctgttt
     7801 cggttcgaca tcgcccggag cgtcaaacat cagatcgcgg gcgctctggt tcttcgggaa
     7861 ggccattacc tcgcggagcg tctcttcgcg ggcgaggatc atcgcgatcc ggtcgatgcc
     7921 gggcgcgatc ccgccgtggg gcggcgcgcc gaactcgaac gcccgcagca ggtgaccgaa
     7981 gcgttcgtca atctctgact gctcgtagcc catcagttgg aacatccgcg actgcagctg
     8041 ctggtcatgg atgcggatcg agccgccgcc gacctcgaag ccgttgagga cgatatcgta
     8101 ggccttcgag cgaacggcac cgacatcgct gtccagcagg gcgatgtcct cgtccatcgg
     8161 cgccgtgaac gggtggtgca gcgagtccca gcgctggttc tccgcgtccc agccaaacag
     8221 cgggaagtca atcacccagc agaaggcagc cacgttagga tctgccagcc ccagttcgtt
     8281 gccgaagcgc tcgcgcaagc gaccgaggac gttggccgcg acatccgcgc tatcggcaac
     8341 cagcaggacc aggtcgccgg tgttggcgcc agtcgcatcg gcgatgccgt ccagctctgc
     8401 ctgcgagagg aacttggcaa tcggtgagcg cgccgagcgg gcgccgtcgt caccttcctc
     8461 gatcgcaagc caggccagcc cgcgcgcccc gaactgcttg gcaaagttgg tcagctcatc
     8521 gatttggcct cggctggagc cgccgcggcc cggaaccgcg atgcctctga caacgccacc
     8581 accggcgacg gcgttggcga agacaccgaa ctcgctgccg gcaaccagac tggagacatc
     8641 agcgatctcc agcccatagc gcaggtcggg cttgtcgctg ccgtagcgca gcatcgcgtc
     8701 ggcgtaggtc aggcgcggga agggcagctc ctgaatggtg aagtcggtga gaccgaccag
     8761 ctcgatgaag agtccctcgg tcagctggat gacgtcttcc atgtcgatga acgacatttc
     8821 gaggtcaagc tgggtgaact ccggctgccg gtcggcgcgc tggtcctcgt cgcggaagca
     8881 gcgcgcgatc tgatagtagc gatccatacc ggccaccatc aggatctgct tcatctgctg
     8941 gggtgattgg ggcagcgcgt agaactggcc cggatagatg cggctcggca ccaggtagtc
     9001 gcgagcgccc tccggcgtgc tcttgatcag gatcggggtt tcgatctcga tgaagtcgcg
     9061 ctcatccaga tagcgccgaa tcgtccggac gatctggtgt cggagcatca ggttccgctg
     9121 catgcgtggc cggcgcagat ccaggtagcg atactccaac cgcagcgatt catcgacgtt
     9181 cgtgtcgtcg ctgatctcga acggcgt
//
LOCUS       DASUGJ010000190         5564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1832501, whole genome shotgun sequence.
ACCESSION   DASUGJ010000190 DASUGJ010000000
VERSION     DASUGJ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832501"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VFV93_04165"
     CDS             <1..1156
                     /locus_tag="VFV93_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5164568.1"
                     /translation="YINQQKHWRPNVQNFVRNWLQDGLKPRPVTRDIEWGIPVPVAGF
                     EHKIMYVWIEAVVGYLSASVEWANNNGQPDAWRAWWTDPDARAYYFIGKDNIPFHAII
                     WPAELMGYSAEMNLPYDIPANEFLNLEGQQFSTSRNWAIWLPDYLASYAADPLRYYLT
                     AVAPETRDSEFTWAGYVERNNNELVATWGNLVNRVISFANRHWEGVVPTPGDLGERDE
                     AILATIAAGFDTVGNLYAKTEFRAALREAIGLAREVNRYLDETSPWFEIKENRERAGT
                     SVYVALRCIDSLKTLLAPVLPHTSQRVHEFLGYERPLFGDLTIEHYAEAEREHDAIVY
                     HPLPEEATEDRWQPSQLEPGRAIQKPTVLFQKLDESVVEEERAKLGVPAS"
     gene            1401..2741
                     /locus_tag="VFV93_04170"
     CDS             1401..2741
                     /locus_tag="VFV93_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5164569.1"
                     /translation="MDDGLPTIPGLLLVAALLLLNGLFVAAEFAFVSVRRSQIQRAAS
                     EGSGSARRVLGALNNLDYYVAAFQLGVTMASIGLGWLGEPVIAHLVEPPIEDLVGGFA
                     PAVAHTVAIAIAFMLITSMHLIFGEATPKSIALQRPTGTALFLSRPMSIFATVFGPAI
                     GLLNKTTRGILRLFGFDPQPITDVPLAAEDLAMSLESSASAGLISRRELSLARNSLRL
                     STLATGDLMVPRNEVDGISYTASREEILHAFARSHHTRYPVYRNGLDDIVGVLNAKEV
                     VLDWTTEGSDWRAHIRAPLVLPETVGIEAALATARSEGATLIVLADEFGGTAGIISVF
                     DIVEFLAGELPDEFEEQQAGVQKLASGAYVFSGLTHVMTLEADLDIALPEVDAHTIGG
                     LVMELLGKVPKAGDELALDGYVARVVSMDGNRVDQVMLTPQPASDRVREAANER"
     gene            2731..4047
                     /locus_tag="VFV93_04175"
     CDS             2731..4047
                     /locus_tag="VFV93_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5164570.1"
                     /translation="MSDDALMTIIALLGTLLLVAGNALFVFHEFSYIVIKPADIRHLQ
                     ERSGRIGRYVAKAAHHLDHYIAVDQLGITATSLAVGWVGQPVVADLFSVPIEAVGAPA
                     GTAAVIGFVLAFVLITALQMIGGELIPKTIALRRTRTVASLVTMPIELISHVLHPIVW
                     VLNGLANLMVRLVGFQPQTEGHNNVLPAEELEVVIQSSAKAGVLKANPNVLRRSLRFS
                     DIQARDVLVPRQDIAAVSLMMTVDEVLETARQFHHTRYPVYDDTIDNIIGLINVKDLL
                     QVRPDGSPAFVERWQRTIRPIPALPVHARIELVLQQLGQEQQQMALLIDEYGGTAGIA
                     TVADVASELFAGSHDIRQAGPRRYLIAGDTSIEAVASELSLTFGDEDHDYDTIAGYVM
                     ASLERVPEVGDEVTDHNTRVRVAAMQGRRVTQVMLDLTEPDEAPDR"
     gene            complement(4172..4570)
                     /locus_tag="VFV93_04180"
     CDS             complement(4172..4570)
                     /locus_tag="VFV93_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX5164571.1"
                     /translation="MASVPQEYRDLLDERPILASLATVQPDGQPQVTPVWIDMEGDLL
                     RVNTAKGRQKHLNMDERPMVTVLSVDPDNVFRYIEVRGKVTRITEEGGDAHIDKLAKK
                     YLDADSYPFRNPAETRLVIYVEPTRVLASG"
     gene            complement(4703..5272)
                     /locus_tag="VFV93_04185"
     CDS             complement(4703..5272)
                     /locus_tag="VFV93_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="HEX5164572.1"
                     /translation="MTIPNGHRIETLEQLRAIIPAPEAGSFAIRKQMEALDERCRAVI
                     AAAPFMLLATAGPDGRCDVSPRGDTPGFVHVVDDRTVVIPDRPGNRRLDSLNNILHNP
                     HAATIFLVPNMEEALRVNGRAWISDDPALLSRMSIQGEPPLVGIVIETEEVCFQFARA
                     LKLSQLWQTDSWRDRESLPAVAQLLGEQP"
BASE COUNT          949 a         1882 c         1763 g          970 t
ORIGIN      
        1 gtacatcaac cagcagaagc actggcgtcc aaacgtccag aacttcgtcc gcaactggct
       61 ccaggacggc ctcaagcccc gtcccgtcac gcgcgacatc gagtggggca tccccgtccc
      121 ggtcgccggc ttcgagcaca agatcatgta tgtctggatc gaagcggtcg tcggctacct
      181 ctcggccagc gtcgagtggg cgaacaacaa cgggcagccg gatgcctggc gcgcgtggtg
      241 gaccgacccg gacgcccgcg cctactactt cattggcaag gacaacatcc cgtttcacgc
      301 catcatctgg ccggccgagc tgatgggcta cagtgctgag atgaacctgc cctacgacat
      361 ccccgccaac gagttcctca acctggaagg acagcagttc agcacctcac gcaactgggc
      421 tatctggctg ccggactacc tggcgagcta cgccgccgac ccgctgcgct actacctgac
      481 ggctgtcgcg ccggaaacgc gcgacagcga gttcacctgg gccggatacg tcgagcgcaa
      541 caacaacgag ctggtggcga cctggggcaa cctcgtcaac cgcgtcatct cgttcgccaa
      601 ccggcactgg gagggcgtcg tcccgacacc cggcgacctc ggtgagcgcg acgaggcgat
      661 cctcgcgacg atcgcggccg gcttcgacac cgtcggcaat ctctacgcga aaaccgagtt
      721 ccgcgccgcc ctgcgcgagg cgatcggcct ggcccgcgag gtcaaccgtt acctggacga
      781 gacctcgccg tggttcgaga tcaaggagaa ccgggagcgg gccggaacat ccgtctatgt
      841 cgcgctgcgc tgcatcgact cgctcaagac actgctggcg ccggtcctgc cgcacacatc
      901 ccagcgcgtc cacgagttcc tcggctacga gcgccccctt ttcggcgatt tgacgatcga
      961 gcactacgcg gaagccgagc gcgaacacga cgcgatcgtc taccacccgt tgcccgagga
     1021 ggcgaccgag gaccgctggc agcccagcca gctcgaaccc ggccgcgcca tccagaagcc
     1081 gaccgtcctc ttccagaagc tcgacgagtc ggttgttgag gaagagcgcg cgaagctggg
     1141 tgtgcccgcg tcttaaatgg agttgtctca cgcgacagcg acatcgcaag tggagatagg
     1201 ttggtcgagg tggtggagcc gggcgagtgc ctggtgcggc gcatagccga ccccactcca
     1261 caaccgagcg acctcaccaa cgggtctgag ggtagattcc cgaacgcaat ggcgcattgc
     1321 aaaaggtggc gtccgtcttg cttgacaggc gactgccagc gccgtacggt gcctcgtgaa
     1381 ggagtgacga cagccagagc atggacgatg gattacctac catacccggc ttgctgctgg
     1441 ttgccgcgct gctgctgctg aacgggcttt tcgtcgcggc tgaattcgca ttcgtctcgg
     1501 tccggcgcag tcagattcag cgggcggcat cggagggcag cggctcggct cggcgcgtgc
     1561 tcggcgcact caacaacctc gattactacg tcgccgcatt tcagctcggc gtcacgatgg
     1621 ccagtatcgg gctcggctgg ctcggtgagc cagtcatcgc ccatctcgtc gagccgccga
     1681 tcgaggatct ggtcggcggg ttcgcgccgg ccgtcgccca cacggtcgcg atcgcgatcg
     1741 ccttcatgct catcacctcg atgcacctca tcttcggcga ggcgacaccg aagtccattg
     1801 cgctgcaacg ccctaccggg acggcgctct tcctctcccg cccgatgtcg atcttcgcca
     1861 cggtcttcgg cccggccatc gggctgctca acaagaccac gcgcggcatc ctgcggctgt
     1921 tcggcttcga tccgcaaccg attaccgacg ttccgctggc ggccgaggac ctggcgatgt
     1981 cgctcgaatc cagcgcctcg gccgggctga tcagccgccg cgagctgagc ctggcgcgca
     2041 actcgctccg gctctcgacg ctggcgaccg gcgatctgat ggtgccgcgc aacgaggtcg
     2101 atggcatctc ttacaccgcc agtcgcgagg agatcctgca cgcgttcgcc cgctcgcatc
     2161 acacgcgcta cccggtctac cgcaacggtc tcgacgacat cgtcggcgtc ctcaacgcca
     2221 aagaggtcgt gctcgactgg acgaccgagg ggagcgactg gcgcgcgcac atccgggcgc
     2281 cgctggtcct gcccgagacg gtcggcatcg aggcggcgct ggcaaccgcc cgctccgagg
     2341 gcgcgacgct gatcgtgctg gccgatgagt tcggcggaac ggccggcatc atcagtgtct
     2401 tcgacatcgt cgagttcctg gccggcgagc taccggacga gttcgaggag cagcaggccg
     2461 gcgtccagaa gctggcgagt ggcgcatacg tcttctccgg tctgacacac gtgatgacgc
     2521 ttgaagccga cctggatatc gcgctgccgg aggtcgacgc ccacacgatc ggcgggctgg
     2581 tcatggagct gctcggcaag gtgccgaagg ccggagatga gctggcgctg gacggctacg
     2641 tcgcgcgggt cgtgtcgatg gacggcaatc gcgtcgatca ggtgatgctg acaccgcaac
     2701 ctgcttctga tcgggtccgg gaggcagcca atgagcgatg atgccctgat gacgatcatc
     2761 gcgctcctcg ggacgctgct gctggtggcc ggcaacgcgc tgttcgtctt ccacgagttc
     2821 tcgtacatcg tcatcaagcc ggccgatatc cggcacctgc aggaacgttc cggccggatc
     2881 gggcggtacg tcgccaaagc cgcccatcac ctcgaccact acatcgccgt cgatcagctg
     2941 ggcatcaccg ccacgagcct ggccgtcggc tgggtcggcc agccggtcgt cgccgatcta
     3001 ttctcggtgc caattgaggc ggtcggcgct ccggccggga cagcggccgt catcggcttc
     3061 gtgctcgcct tcgtcctgat caccgcgctc cagatgatcg gcggcgaatt gatccccaaa
     3121 acgatcgccc tgcgccggac acggacagtg gcgtcactgg taacgatgcc gatcgagctc
     3181 atctcgcacg tcctgcatcc cattgtctgg gtgctgaacg ggctggccaa cttgatggtt
     3241 cggctggtcg gctttcagcc gcagaccgag ggacacaaca acgtgctacc ggccgaggag
     3301 ctggaagtcg tcatccagtc cagcgcaaag gccggcgtcc tgaaggcgaa tcccaacgtc
     3361 ctgcgccgct cgttgcgctt ctccgacatc caggctcgcg atgtcctggt gccacggcag
     3421 gacatcgccg ccgtcagcct gatgatgacg gtcgatgagg tgctggaaac cgcccgccag
     3481 ttccaccaca cccgctaccc cgtctatgac gacacgatcg acaacatcat cgggctgatc
     3541 aacgtgaagg atctgctcca ggttcgcccg gatggctcac cggccttcgt cgagcgctgg
     3601 cagcggacga ttcgcccgat cccggccctg ccggtccatg cgcggatcga gctggtcctc
     3661 cagcagctcg gccaggagca gcagcagatg gcgctgctga tcgatgagta cggcgggacg
     3721 gccgggatcg cgacggttgc tgatgtcgcc tccgagctgt tcgccggctc ccacgatatc
     3781 cgtcaggccg gcccgcgccg ctatctgatt gccggcgaca ccagtatcga agccgtcgcg
     3841 tccgagctct cactgacctt cggcgacgag gaccacgact acgacacgat cgccggctac
     3901 gtgatggcct cgctggaacg agtccccgaa gtcggcgacg aagtcaccga ccacaacacc
     3961 cgcgtgcgcg tcgccgccat gcagggccgc cgcgtcaccc aggtcatgct ggatctgacc
     4021 gagcctgacg aggcgccgga tcggtaggtt catatccagc caaacccctc cccaaccgct
     4081 cccgtgaaac gggaggggct tttgattcgt gcggcatgaa gccccctctc tccgtggggg
     4141 aggtggttgg ggaggggttt ctgagacgtg cctaccccga cgccagcacc cgcgtcggct
     4201 cgacgtagat caccaatcgg gtttcggcgg ggttgcggaa tgggtaggag tcggcgtcaa
     4261 ggtacttctt ggccagcttg tcgatgtggg cgtcgccgcc ttcctcggtg atacgcgtca
     4321 ccttgccgcg cacctcgatg tagcggaaga cgttgtcggg atcgaccgac aggaccgtca
     4381 ccatcggacg ctcgtccatg ttgaggtgct tctgacggcc cttcgcggtg ttgacccgca
     4441 gcaggtcgcc ctccatgtcg atccagaccg gcgtgacctg cggctggccg tccggctgga
     4501 cagttgccag gctggcgagg atgggtcgct cgtcgagcag atcacgatat tcctgtggga
     4561 cagatgccat tcgtcgttcc tccatgagac tcatcgattt cggcggcaac ctccgcgaga
     4621 cacggaaagg ccgccagagg tatcaaccaa ctgtcgaggc aagaaattcc ttgcaacacc
     4681 agggacagaa cctcgcttgt ggctatggct gctcgcccag cagttgggcg acggccggca
     4741 gcgactcacg gtcgcgccag ctatcggtct gccagagttg cgagagcttg agggcgcgtg
     4801 cgaactggaa gcagacttct tcggtctcga tgacgatacc gaccagcggc ggctcaccct
     4861 gaatgctcat ccgcgacaaa agcgccgggt catcgctgat ccaggcgcga ccgttcacgc
     4921 gcagcgcctc ctccatgttc gggacgagga agatcgtcgc cgcgtgcggg ttatggagga
     4981 tgttgttcag cgaatcgagc cggcggttgc ctggccggtc cgggatgacg accgtccgat
     5041 cgtcgacgac atgcacgaat ccgggcgtgt cgccgcgcgg cgagacatcg cagcggccgt
     5101 cggggccggc cgtcgccagc agcatgaacg gcgcggcggc gatcaccgcc cggcagcgct
     5161 cgtccagcgc ctccatctgc ttgcggatcg cgaaggagcc cgcctccggc gccgggatga
     5221 tcgcccgcaa ctgctccagc gtctcgatgc gatgcccgtt cggtatcgtc acaggttggt
     5281 tctccctcac ggaatccaaa gcacgcccta ccggtagggg cgaggcaccg actcgccctt
     5341 cgtgtctctc ccaaacggct cccgtggaac gccaggcgag tcagtgcctt gcccccgctt
     5401 gtgggcagcc tgccgcgtgc aacgcgagcg cgtcctactg gtccggcggt tcctcgtcct
     5461 caccggcttc gcgcatcggt tcgctggcca gctcttcgac cgattcgtgc ttgccttcgc
     5521 tgcgcgcaat cttgcgagcc ggggcacggg tttcgccgtc gatc
//
LOCUS       DASUGJ010000191         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1136882, whole genome shotgun sequence.
ACCESSION   DASUGJ010000191 DASUGJ010000000
VERSION     DASUGJ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136882"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFV93_04190"
     CDS             complement(<1..204)
                     /locus_tag="VFV93_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164573.1"
                     /translation="MADEHSVANRRTLPVDLTELTYAAESHDAEMRTVIDRETGELVL
                     VFETSFQIADEYVSSQSEAIEVSS"
     gene            280..669
                     /locus_tag="VFV93_04195"
     CDS             280..669
                     /locus_tag="VFV93_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5164574.1"
                     /translation="MQIRGIAEIVLNVHDKQAALAFYRDLLGLEVISRPEMPNVFLKA
                     GPGQAGVPQMIVLVPLPDGADTFATPRTLHHLAFEVAPEDFDVVAEHLRVNGYTPRGG
                     QHPVIASRTQYVDDPDGNEVEIICAEA"
     gene            836..>2149
                     /gene="lysA"
                     /locus_tag="VFV93_04200"
     CDS             836..>2149
                     /gene="lysA"
                     /locus_tag="VFV93_04200"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5164575.1"
                     /translation="MTAERAAGGALSVGGVPLADLAREFGTPLYVYDVATIREQCRRY
                     TRAFTDAYPRPRVVYAGKAWMSHALLEIIAAEGLSLDVVSAGELALALACDFPPERIN
                     FHGNNKTPEELRMALDAGIACIIIDNFDEIDLLATLTAGRADPQDVLIRLNPGIDVHT
                     HDYRKTGIPDSKFGLGIATGDAERAVERIVGIDGLRLRGYHAHIGSQIFEIDPFVDTV
                     DTIFAFAAAMRDRFGIVPDEMSPGGGYGIVYEPGDPQPRIEEYAGTVARAALAAAERH
                     DLPDPILTIEPGRSIVGMAGVAVYTLGARKVIPGVRTYVSVDGGMADNIRPALYGAGY
                     AAELVARPTSDDVQMVTIAGKYCESGDVLIDRVGLPPFEPGDLLAVPATGAYCLAMAS
                     NYNLALRPAVVFVEDGRGWIVQRRETYNDLLLRDIVEPVPVSFASG"
BASE COUNT          389 a          639 c          710 g          411 t
ORIGIN      
        1 ggaactgacc tcgatcgctt cggactgtga cgaaacgtac tcgtcggcga tctggaagga
       61 cgtctcaaaa accagaacga gttcgcctgt ctcgcgatcg atgaccgtgc gcatttcagc
      121 atcgtgcgac tcagcagcgt acgtgagttc cgtaagatcg actggcagcg tgcgacggtt
      181 tgcgacactg tgttcatccg ccacgatcga ctcctttcag ctagcatggt gacttcggga
      241 gaactggaag cggtgatgat gacatcgagg agaacggcaa tgcagatacg gggcatcgcg
      301 gagatcgtac taaacgtcca cgacaagcag gcggcgttgg cattctatcg tgatctgctg
      361 gggctggagg ttatctcgcg gccggagatg ccgaatgtgt tcctgaaggc gggaccgggg
      421 caggcgggag tgccgcagat gatcgtgctg gtgccgctgc cggatggcgc agatacgttt
      481 gccacgccga ggacgttgca ccacctggcg ttcgaggttg caccggaaga ctttgacgtg
      541 gtcgccgaac atctccgggt gaatggctac acgccacgtg gcgggcagca cccggtcatt
      601 gcctcgcgga cgcagtatgt ggacgatccc gacggtaacg aggttgagat catctgcgct
      661 gaggcgtgac ataacctgcg cgcgttgacg gcaaagcgga tatacttcgc gcacgctggc
      721 gaaggtggag acggtcacga cggaaacggg aaggccagtt ccgtcgatcg cgcgaccgtc
      781 acggtgtccc agcctggtta gtccggttac cttgggggtc atgatgctct ggccgatgac
      841 ggccgaacgt gccgccggag gcgcgctgtc ggtcggcggt gttccactcg cggacctggc
      901 gcgggagttc gggacgccgc tgtacgtcta cgatgtcgcc acgatccgcg agcagtgtcg
      961 ccgctacacc cgggcgttta ccgatgccta cccacggcca cgcgtcgtct acgccggcaa
     1021 ggcgtggatg tcccacgcac tgctggagat catcgctgcc gaggggctgt cgctggatgt
     1081 cgtctcggct ggcgaactgg cgctggcgct ggcctgcgat ttcccccccg aacggatcaa
     1141 ctttcacggc aacaacaaga cgcccgaaga gctgcgaatg gcgctcgatg ccgggatcgc
     1201 ctgcatcatc atcgacaact tcgacgagat cgacctgctg gcgacgttga cggccggacg
     1261 ggccgacccc caggatgtgc tgatccggct caaccccggt atcgacgtgc acacgcatga
     1321 ctaccgcaag accggcattc cggactccaa gttcggcctt gggattgcga ctggcgatgc
     1381 cgagcgtgcc gtcgagcgga tcgtggggat cgacgggctg cgcctgcgag gctaccacgc
     1441 gcacatcggc tcgcagatct tcgagatcga cccgtttgtc gataccgtcg acacgatctt
     1501 cgccttcgcc gccgcaatgc gcgaccggtt cgggatcgtg ccggacgaga tgagccccgg
     1561 tggcggctac ggaatcgtct acgagccggg cgacccgcag cctcggatcg aggaatacgc
     1621 cggaaccgtc gcgcgagcgg cgctggcggc ggccgagcgg cacgacctgc ctgatccgat
     1681 cctgacaata gagccgggcc gctcgatcgt cgggatggcc ggtgttgccg tctacacgct
     1741 cggagcgcgc aaggtcatcc ccggtgtgcg aacatacgtc tcagtcgatg gcgggatggc
     1801 tgataacatt cggccagcac tctacggtgc cggatatgcc gctgaactgg tcgcgcgccc
     1861 gacgagcgac gatgttcaga tggtcacgat cgccgggaaa tactgcgagt ccggtgatgt
     1921 tcttatcgat cgggttggat tgccgccgtt cgagccggga gatctgctgg ctgttcccgc
     1981 caccggcgcc tattgccttg cgatggcgag caattacaat ctcgctctcc ggccggctgt
     2041 tgtctttgtt gaggatggac gtggctggat cgtccagcgt cgagagacgt acaatgatct
     2101 cctcctgcgg gatatcgtag aaccagtacc tgtttcgttt gcgtcaggc
//
LOCUS       DASUGJ010000192         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1555530, whole genome shotgun sequence.
ACCESSION   DASUGJ010000192 DASUGJ010000000
VERSION     DASUGJ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555530"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /gene="uvrA"
                     /locus_tag="VFV93_04205"
     CDS             <1..1547
                     /gene="uvrA"
                     /locus_tag="VFV93_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5164576.1"
                     /translation="VREAEQFLQSLQLTEKEAAIGDKVLREIQKRLKFLSDVGLDYLT
                     LDRLSSTLSGGESQRINLATSLGSSLVDTLYVLDEPSIGLHSRDNLRLINILRQLRDQ
                     GNTVLVVEHDEDMIRVGDTIVDLGLGAGEQGGRVVFAGPLENLLLDQRSLTSKYLRGD
                     LAIPVPSGRRRGSGQKLRVLGATEHNLKSIDVEIPLNTLTCITGVSGSGKSTLVHDVL
                     YAAVKRAKGGTEKRVGAHRKIEGTEFVSDVVLVDQTPIGRTPRSNPVTYLKAFDPIRE
                     IFAATKDAKSRGLTASHFSFNVPGGRCEACQGEGEVRVEMQFLADVFVPCDQCDGRRF
                     KPQILEVRYRAKNINQVLDLTVREALGFFSTSPKVLRRLQVLDEIGLGYLRLGQPATT
                     LSGGEAQRIKIASHLSSRTGDRILYVLDEPTTGLHFDDIAKLLTAFKKLLEAGHTLVV
                     IEHNLDVLKTADYIIDLGPEGGHEGGRVIATGTPEQIAKAPGSHTGHFLQPVLANGRS
                     HAYALE"
     gene            1549..>2743
                     /locus_tag="VFV93_04210"
     CDS             1549..>2743
                     /locus_tag="VFV93_04210"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5164577.1"
                     /translation="MSAAVALVGCLYVVSGFSRTVLYVVSGFSRTVSYVVSGFSRTVS
                     AQQYSFEEVASGLKHKDADTRLRAIQILKDADYQEAAGPIGDLLADVDDRVQLAALDA
                     ERSLFTLRPVSRKKMVGFVIEQRSTSGSGDVAAEGQLALKARLVPPSVLGGLVIALSD
                     RNPQVRSQAIDLAGLLAPIACPVRPKANRTGCDPVGNALIENINSREAPVRRAAMRTL
                     GRLQYPQAVQALMDQLSYHEKGPDAQAALEGLAGIGHVASVSTLEELLKSANTGVRTL
                     AIEGLARADARDALPALQQMGQSERSAGVLLAMHYASLKLGAPGSTPDHLVAALRNET
                     LKPAAIRYLLDLAPTMAPTLAQWLGDQDPEVRRLIADILGFSGDSTVVPVLAQAARAT
                     DPDLVL"
BASE COUNT          471 a          880 c          913 g          479 t
ORIGIN      
        1 cggttcgcga agccgagcag ttccttcagt cgttgcagct gaccgaaaag gaagcggcca
       61 tcggcgacaa ggtcctcagg gaaatccaga aacggctgaa gttcctgagc gacgtcgggc
      121 tcgactacct gacgctcgac cggctgtcgt cgacgctctc cggcggcgaa tcgcagcgca
      181 tcaatctggc gacgtcgctc ggctcgtcgc tcgtcgacac gctgtacgtg ctcgacgagc
      241 cgtccatcgg tttgcactcg cgcgacaacc tgcgcctcat caacatcctc cggcagcttc
      301 gcgatcaggg caacacggtg ctggtggtcg agcacgacga ggacatgatt cgcgtcggcg
      361 acacgattgt cgatctgggc ctcggggcgg gcgagcaggg cgggcgcgtg gtgttcgccg
      421 gcccgctcga gaacctgctg ctggatcagc gctcgctgac ctcgaagtat ctccgcggcg
      481 atctggcgat cccggtgcct tcgggccggc gtcgcggcag cggccagaaa ctgcgtgtgc
      541 tcggtgcgac ggaacacaat ctgaagagca tcgacgtcga gattcccctg aacacgctga
      601 cctgcattac aggcgtcagc gggtctggca agtcgacgct cgtgcacgac gtgttgtatg
      661 cggccgtcaa gcgggcgaaa ggcggcaccg agaagcgcgt cggtgcgcat cggaagatcg
      721 aagggaccga gtttgtcagc gatgtcgtgc tggtcgatca gacgccgatc ggccgcacgc
      781 cccggtcgaa cccggtgacc tacctgaagg cgttcgatcc catccgcgag attttcgcgg
      841 cgaccaagga cgccaagtcg cgggggctga cggcgagcca cttctcgttc aacgtgccgg
      901 gcggtcgctg cgaggcgtgc cagggcgaag gcgaagtgcg cgtcgagatg cagtttctcg
      961 ccgacgtgtt cgtgccctgc gatcagtgcg acggccggcg cttcaaaccg cagattctcg
     1021 aggtgaggta ccgggccaag aacatcaacc aggtgctcga cctcacggtg cgcgaagcgc
     1081 tcgggttctt cagcacctcg cccaaggtgc tgcgccgcct gcaggtgctc gacgaaatcg
     1141 ggcttggcta tctgcggctc ggtcagccgg cgacgacgct ctcgggtggc gaggcccagc
     1201 gcatcaagat cgcgtcgcac ttgtcgtcgc gaacgggtga ccgcatcctg tacgtgctcg
     1261 acgagccgac gaccggtctc cacttcgacg acatcgccaa gctgttgacg gcgttcaaga
     1321 aacttctgga ggcggggcac acgctggtgg tgatcgagca caacctcgac gtgctgaaga
     1381 ccgcggacta catcatcgat ctcggcccgg aaggtggaca cgaaggcggc cgcgtcatcg
     1441 cgaccggcac gcccgaacag atcgccaagg cgccggggtc gcacacggga catttcctcc
     1501 agccggtcct cgcgaatgga cggtcgcatg cgtatgcgct tgaatagggt gagcgctgcg
     1561 gtcgcgttgg tgggctgcct gtacgtagtg tccggcttca gccggaccgt gttgtacgta
     1621 gtgtccggct ttagccggac cgtgtcgtac gtagtgtccg gctttagccg gaccgtgtcg
     1681 gcccagcagt actccttcga agaggtcgcg tccggcctca agcacaagga cgctgacacg
     1741 cggctgcggg cgatccagat cctgaaggac gccgactacc aggaagccgc cgggccgatt
     1801 ggcgacttgc tggccgacgt tgacgaccgc gtccagctgg cggccctcga cgccgagcgg
     1861 tcgctcttca cgcttcggcc cgtgtcgcgc aagaagatgg tgggcttcgt catcgaacag
     1921 cggagcacct ccggctcggg cgacgtggcg gccgaaggcc agctcgcgct gaaggcccgc
     1981 ctggttccgc catccgtcct cggcggcctg gtgattgccc taagcgatcg caatccgcag
     2041 gtcaggtctc aggccatcga tctggcaggc ttgctcgcac cgattgcctg cccggtccgc
     2101 ccgaaggcga atcgcaccgg ctgcgatccg gtcggtaacg cgctgattga gaacatcaat
     2161 tcccgggaag cacctgtacg gcgtgccgcc atgagaacgc tcggccgcct ccaatatccg
     2221 caggcggtgc aggcgttgat ggaccagttg agctaccacg agaaagggcc cgatgcgcag
     2281 gccgcccttg aaggtctcgc tggcatcggc catgtcgcgt cggtctcgac gttggaagag
     2341 ctgctcaaga gcgcgaatac aggcgtccgt acgctggcca tcgagggtct tgcgcgcgca
     2401 gacgcgcgcg atgcgctgcc ggcgttgcag cagatggggc agagcgagcg ctctgccggc
     2461 gtgctcctcg cgatgcacta tgcgtcgctc aagcttggcg cgccaggaag tacacccgat
     2521 catctcgttg ccgcgcttcg gaacgagacg ctgaaaccgg cggcgatccg ctatctcctc
     2581 gatctggcgc cgacgatggc tccgacactc gctcagtggc tgggtgacca ggatccagag
     2641 gtgcgtcgcc tgatcgccga catcctcggt ttctcgggag attcgaccgt cgttccagtc
     2701 ctcgcccagg cggccagggc caccgatccc gacctggtgc ttg
//
LOCUS       DASUGJ010000193         5791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1832735, whole genome shotgun sequence.
ACCESSION   DASUGJ010000193 DASUGJ010000000
VERSION     DASUGJ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832735"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VFV93_04215"
     CDS             <1..938
                     /locus_tag="VFV93_04215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5164578.1"
                     /translation="GVRLVKLPTIRNKYLDTIVHSTIAAFDAIVRRYDVVYVFGVGNS
                     PVCALLRLGRLPVLLNVDGLDWQRDKWPVAARWCLRLAERIAVRVANRTITDSPNVRE
                     YYRDQRGVDLDYIPYGATPIYTPPNGTLEAHGLDARNYFLYVGRLEPENHVHDLVTAA
                     RSAGGALPVVVVGDAPYADDYKTALRRDAGEHVRFTGAIYGSGYWELNQNAYAYVFPV
                     VASGTHPALIEAMACGNCVLARDTPDNRHVGGDTIRYFRDAEELSALMRWAEASPSDV
                     ARLGELAALRVREVFDWERITNEYLDLTRQVLGRP"
     gene            950..2119
                     /locus_tag="VFV93_04220"
     CDS             950..2119
                     /locus_tag="VFV93_04220"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5164579.1"
                     /translation="MTRTDPALLRAATEYVTALALDGPHDGAWIDQPRGFEALKEASS
                     IHGVGPLLGLRVEAGEIAPPDPLASWLVEQVSRNRTRLTRMRAELLETLAALAERGFV
                     AMPLKGGALLLESVERVVWRTFADLDLLVLDTADRRQDLDLALAHAGYCLGGVSWKHR
                     SYSACAPGPPLVIGDGEHPDNPRDVEVHAAVVEMFRGFRWDLTPLLLREPGERAGRQV
                     PSDAALALHLAIHASVSILEGTAKAINLIDLARAIELVGPMPLYLAARDAGLATHARF
                     LYPAAALTARETHNPNCLALSDMLRPWVTAQMAGWAEAVSLYHISWAGRDDRSALDRY
                     GLWARSRAERARMLATTLLPAPSVLASENLGGTGPISVAKGYGRHYRRLASRIIR"
     gene            2362..2814
                     /locus_tag="VFV93_04225"
     CDS             2362..2814
                     /locus_tag="VFV93_04225"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5164580.1"
                     /translation="MQTSAVPTTVGVGVGPRALAIIIDAVAIGIVSFILQMLAGGDSG
                     AGTLVGCFFAIASLAYYPVMEKVYGASLGKMVMGLKVVTETGAPLDWTAAILRTLLRL
                     IDGLFVYLIGAILVWTSPTNQRLGDKVARTLVVRKDQTVVGSSPTSRF"
     gene            complement(2885..3532)
                     /locus_tag="VFV93_04230"
     CDS             complement(2885..3532)
                     /locus_tag="VFV93_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5164581.1"
                     /translation="MATAQRTKAVVQEQRIVLHNISWKTYECLLADHQDASAPRFTYD
                     RGELEIMSPLREHEQTNRSIASLVEIVAEEQDIEFDNLGSTTFRRQDMERGFEADSCF
                     YIQNESAIRGKRFIDLETDPPPDLVIEVDITRSSLDKQPVFAAIGVNEVWRYDGGSLT
                     ILLLDGDDYRPSEHSRALPGVTATDVNRFVAAIGSSGRTSWLRRVRRWARDLNQR"
     gene            complement(3710..5299)
                     /locus_tag="VFV93_04235"
     CDS             complement(3710..5299)
                     /locus_tag="VFV93_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5164582.1"
                     /translation="MPESTNADVVVRELLGHGVGTIFTVSGNQILPLIDAAGRAGVHL
                     VHMRHETAAVYAAIAAAEVTGGISVALVSAGPGFLASLQGLGVAMSMELPILLLSGAS
                     PAGQRGLGAFQELDQRTIASAVCKGSVELSPHDPIGSVLDAWSMAVSDIPGPVHLSLP
                     SDVLAAPDDRERPNLPDPATWRFDEEITRRLDEMAAALSKAERPLVIARPSIGRPSRD
                     ASLLGLTGALGIGPIVTESPRGLSDLKYSEIFARLRESDCLLTIGPADFSAGFLAEAT
                     IGNPRDVLLIDAPGDPTPRRADHLHVRAPTSQAVRYLAGRVIRHQPVADDWAALWPIP
                     APTPAPPQTWGPMHPLAVSEALRAVLQPEDVVILDGGEFCQWIRYGLRDLPNRLLWNG
                     KLGAIGGSIPMAIGAATVAKAGRVYVLLGDGSAGYHLSEFETAAREHLRFTAIIGNDA
                     RWAAEWHMQIERYGPDSTFSTSLTAARYDLAAHGFGGVGVLVDEPNALRGALETALES
                     PTATCINAIVAAERSPATVTH"
     gene            5512..>5791
                     /locus_tag="VFV93_04240"
     CDS             5512..>5791
                     /locus_tag="VFV93_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX5164583.1"
                     /translation="MTATAATTTANLVRVGRLEEIESPTVVSGGRHGIAVFISEGRPF
                     AVDNRCPHMGFPLHKGSLRDGILTCHWHHARFDLESGGTFDPWADDVRT"
BASE COUNT          947 a         1773 c         2015 g         1056 t
ORIGIN      
        1 ggggtgttcg gctcgtcaag ctgccgacga tccgcaacaa gtacctcgac acgatcgtcc
       61 attcgacgat cgcggctttc gatgcgattg tccggcgcta cgacgtggtc tacgtcttcg
      121 gggttgggaa cagtccggtc tgcgcgctcc tgcgcctcgg caggttgccg gtcctgctga
      181 acgtcgacgg gctggactgg cagcgtgaca agtggccggt ggcggcccgc tggtgcctgc
      241 ggctggccga acggatcgcc gtccgcgtcg cgaaccgaac gatcaccgat tcaccgaacg
      301 tgcgcgagta ctaccgcgat caacgcggcg tcgatctgga ctacatcccg tacggggcga
      361 ccccgatcta cacgccgccg aatgggacac tggaagcaca cgggctggac gcgcggaact
      421 acttcctcta cgtcggccgg ctcgaaccgg agaaccatgt tcacgatctc gtgacggccg
      481 cgcggtcggc gggtggggcg ctgcccgtgg tggtcgttgg ggatgcgcca tacgcggacg
      541 actacaagac cgctctccgc cgcgacgcgg gggagcatgt gcgcttcact ggcgccatct
      601 acggatccgg ctactgggaa ctgaaccaga acgcctacgc gtacgtcttc ccggtcgtcg
      661 cgagcgggac gcatccggcg ttgatcgagg cgatggcgtg cggcaactgc gtcctggcgc
      721 gcgacacgcc ggacaaccgg catgtcggcg gcgacacgat ccgctacttt cgcgatgctg
      781 aggagttgtc ggcgctgatg cgctgggcgg aagcgtctcc ctcggacgtc gcccggctcg
      841 gggaactggc cgcgttgcga gtgcgcgagg tgttcgactg ggaacgcatc acgaacgagt
      901 atcttgacct gacacggcag gtgctggggc ggccgtgagc gacccgcgca tgaccaggac
      961 agacccggcg ctcctgcgcg cagcgacgga gtatgtcacc gcgcttgcgc tcgacggacc
     1021 gcacgatggg gcttggatcg accagccccg agggttcgag gcgctcaagg aggccagctc
     1081 gatccacggg gtcgggccgt tgcttgggct gcgggtcgaa gccggcgaga ttgcgccgcc
     1141 cgatccgctc gcgagctggc tcgtggagca ggtcagccgg aatcgtacgc ggctgacgcg
     1201 gatgcgggcc gagttgctgg aaacgctggc ggcgctcgcc gaacgcgggt ttgtggcgat
     1261 gccgctcaag ggcggggcgc tgctgctcga atcggtcgag cgtgtcgtct ggcgcacctt
     1321 cgcggacctc gacctgctgg tgttggacac agcggatcgt cgtcaggatc tggatctcgc
     1381 gctggcgcac gccggttact gtctcggggg cgtttcctgg aaacaccgca gctactcggc
     1441 gtgtgcgccc ggcccgccgc tggtgatcgg cgatggtgag catccggata acccgcgaga
     1501 cgtcgaagtc cacgcagccg tcgtcgagat gttccggggc ttccgctggg atctgacgcc
     1561 gttgctccta cgagagcctg gggaacgggc aggcaggcag gtgccgtcgg atgccgcgct
     1621 ggcgctccat ctcgcgatcc atgcctcggt ttcgatcctg gagggaaccg cgaaggcgat
     1681 caacctgatc gacctggcgc gggcgattga gttggttggg ccgatgccac tctacctggc
     1741 cgcccgcgat gccggcctgg cgacacacgc gcgcttcctc tatccggccg cggcgctgac
     1801 ggcgcgggaa acgcacaatc ccaactgcct tgcgttgagc gacatgctcc ggccctgggt
     1861 gactgcgcag atggccggct gggccgaggc cgtctcgctg tatcacatct cctgggctgg
     1921 ccgcgacgac cggtcggcgc tggaccggta tggcctttgg gcgcggagcc gtgccgaacg
     1981 ggcacggatg cttgcgacaa cgctcctgcc ggcgccatcc gtgctggcga gcgaaaacct
     2041 gggtgggaca ggtccgatat cggtcgcgaa aggctatggg cggcactacc gccggttggc
     2101 gtcgcggatc atccgataat cagatcgtga ttcaacgcaa tagttgggaa cgcccgaaat
     2161 ccatgacctg cgtcgattga cctgcgaacg tatgccgtgc tacgctcatg gcagacgctt
     2221 tgttgtcagc aagagacgtg agtccacgcg gaattccgtc gaccgagtga acaaccgtgc
     2281 cgcctgttcg gcgcgatttc ggatcaatcg gtagacggtc cgccgggatt tcgcgtatct
     2341 gttggaaggg gttgccgcca gatgcagacg tccgccgtac caacgaccgt gggggtcgga
     2401 gttgggccgc gcgcactcgc gatcatcatt gacgcggttg cgattggaat cgtctcgttc
     2461 atcctgcaaa tgctcgctgg aggtgacagc ggggcgggga cactcgtcgg ctgtttcttc
     2521 gcgatcgctt cgctggccta ctatccggtc atggagaagg tgtacggtgc gtcgctcggg
     2581 aagatggtga tgggcctgaa agtcgtcacc gagactggcg cgccgctcga ctggactgcc
     2641 gctatcctgc ggacgctact acgcctgatc gatggtttgt tcgtctatct gatcggcgcg
     2701 attctggtct ggacttcgcc gacgaatcag cggctgggtg acaaggtcgc caggacgctg
     2761 gtcgtgcgca aggaccagac tgtcgttggt tcatcgccga cgtcacggtt ctagactcca
     2821 gacgccgtta ccgacttctt ttgcgagggt cgccaggcag tcaactgcct ggcgttttcc
     2881 tcgtctaccg ttgattcaga tccctcgccc atctgcgaac ccgtcttagc cagctcgttc
     2941 gacctgacga cccaatcgcc gcgacgaatc ggttcacgtc tgttgcagtg acgccaggta
     3001 gggcccggct gtgttcactc gggcgatagt cgtcaccgtc gaggagaagg atcgtcagac
     3061 tgcctccgtc atatcgccac acttcgttga ctccaatcgc ggcgaagacc ggttgcttgt
     3121 cgagcgacga acgggtaatg tcgacctcaa tcacaagatc ggggggtggg tcagtctcca
     3181 gatcgatgaa gcgcttgcca cggatcgcgg attcgttctg aatgtagaag cacgagtctg
     3241 cttcgaagcc acgctccatg tcctgccggc ggaacgttgt cgagcccaga ttatcgaact
     3301 cgatatcttg ttcctccgct acgatctcaa caagcgaggc gatggatcta tttgtctgct
     3361 catgctcgcg caatgggctc atgatctcca gctccccgcg atcgtaggtg aatcttgggg
     3421 cgctggcgtc ctggtggtcg gcgagcaggc actcatacgt cttccaactg atgttgtgga
     3481 ggacgatccg ttgctcctga acgactgcct tcgtccgctg ggctgttgcc atcgtgccac
     3541 ccgctctgtc cgcttccgtt cgaaccagtc gatcgttgcg ctctattatg aaccaacccg
     3601 ccggtgcggt aacgcgatcg cgctcggcca tccgattggc tgctcggggg cgcacgtcac
     3661 gccgacgctg tcctcaccat cagccctctg gacgttgggg cgagagcgct caatgcgtta
     3721 ccgtcgcggg gctgcgttcg gcggcgacaa tggcgttgat gcaggtcgcc gtcggtgatt
     3781 cgagggccgt ctcgagggcg ccgcgtaggg cgttgggctc atcgacgagg acgccgacgc
     3841 cgccgaagcc gtgggctgcg aggtcgtagc gggcagctgt caggctggtg gagaaggtgc
     3901 tatcgggccc gtagcgctcg atctgcatgt gccactcggc tgcccagcgg gcgtcgttgc
     3961 cgatgatcgc tgtgaaacgc aggtgttcgc gggcggcggt ttcgaattcg gagagatggt
     4021 agccggccga gccgtcgccg agcaggacgt agacgcggcc tgccttcgcg acggtggccg
     4081 cgccgattgc catcgggatg ctgccgccga tcgcgccgag cttgccgttc cagagcaggc
     4141 ggttgggcag gtcgcgcagg ccatagcgaa tccactggca gaactcgccg ccgtcgagga
     4201 tgacgacgtc ctccggctga agcaccgcgc ggagtgcctc gctaacggcc agcgggtgca
     4261 tcggacccca ggtctgtggc ggggctggtg tcggggccgg gatcggccag agtgccgccc
     4321 aatcatcggc gaccggctgg tgtcggataa cgcgaccggc caggtaacgt accgcctggc
     4381 tggtcggcgc gcggacgtgc aggtgatcgg cgcggcgtgg ggtgggatcg cccggggcat
     4441 cgatcagcaa cacgtctcgt ggattgccga tcgtcgcctc ggccaggaag ccggctgaga
     4501 agtcggccgg cccgatcgtc agcaggcagt cggactcgcg tagccgcgcg aagatctcgc
     4561 tgtacttgag gtcgctgagc ccacgcgggg actcggtgac gatcgggccg atgccgagcg
     4621 cgccggtcag gccgaggagc gacgcgtcgc ggcttggccg gccgatcgac gggcgagcga
     4681 tgacgagtgg acgttcagcc ttgctgagtg cggcggccat ctcgtccagc cggcgagtga
     4741 tctcctcgtc gaaccgccac gtggcgggat cgggcaggtt tggcctctca cgatcgtccg
     4801 gggctgccag cacatcggaa ggcagcgaga ggtgaaccgg gccgggaatg tcggagacgg
     4861 ccatgctcca ggcatcgagg acggagccga ttgggtcgtg gggagagagc tcgaccgagc
     4921 ctttgcagac ggctgacgcg atcgtgcgtt gatcgagctc ctggaatgcg ccgagaccgc
     4981 gctggccggc cggcgaggct ccgctgagca gcaggatggg cagttccatg ctcatcgcga
     5041 cgccgagccc ctgaagcgag gcgaggaagc cggggccggc agagacaagc gcgacgctga
     5101 ttccgccagt gacctcggcg gcagcgatcg ctgcgtagac agctgctgtc tcgtggcgca
     5161 tgtggacaag gtgaacaccg gcccggccgg cggcgtcgat cagcggcagg atctggttgc
     5221 cggagacggt gaagatcgtg ccgacaccgt ggccgagcaa ttcgcgaaca acgacatcag
     5281 cattggttga ttccggcatc gtcagatccc ttctcgtgtg acagcgcgat gcacgagcgt
     5341 acgggatgtg ccggtcggcc gcaagtcgtg gtgctccctc caggacccac cgaagagctg
     5401 atgatgtacg acattcttcc gatgactgga tcaggcacgc gtactaggat gacggccatc
     5461 agcgagcagc gtgggcggcc tcggcgtgtc gcgcaggact ggagggaccg gatgacggca
     5521 accgcagcga cgacgacagc gaacctggta cgggttggca ggctggagga aatcgagagc
     5581 ccgaccgtcg tgtcgggagg acggcacggc attgccgtgt tcatcagcga ggggcggccg
     5641 ttcgccgtcg acaatcgctg cccgcacatg ggctttccgc tgcacaaggg cagcctgcgc
     5701 gacggcatcc tgacctgtca ctggcatcat gcccgcttcg accttgagag tggcgggacg
     5761 ttcgatccgt gggccgatga tgtccggacc t
//
LOCUS       DASUGJ010000194         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1971542, whole genome shotgun sequence.
ACCESSION   DASUGJ010000194 DASUGJ010000000
VERSION     DASUGJ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971542"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFV93_04245"
     CDS             complement(<1..415)
                     /locus_tag="VFV93_04245"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009312 - oligosaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009877 - nodulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5164584.1"
                     /translation="MREHDSLPAGYFERVYAENDDPWGFATRPYEARKYAATLATLPR
                     ERYRSAFEIGCSIGVLTEQLAARCDRLLAVDVAERALEQARTRCRHLPQVRFARMAVP
                     AEFPEEVFDLILVSEVGYYWDWDDLRRAQRLIVEHL"
     gene            complement(408..1544)
                     /locus_tag="VFV93_04250"
     CDS             complement(408..1544)
                     /locus_tag="VFV93_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5164585.1"
                     /translation="MTGSLLATAARIADQIAELAAASDDERAFPVEEFRLMGEAGLLV
                     APLPRDLGGCGLDGSPATALELLLLLKEIGRGNLSVGRVYEGHVNAIQLIQTFGTVEQ
                     LTRTAADARDGRLFGVWNTEAQDGVRITPLAGGRYRLAGAKTFASGAGHVQRPIVPGA
                     LPDGGWQMVVVPIERVAVEIDPDWWQPIGMRASASYRVDFSGVEVNASDLIGRPGDYH
                     RQPWFAGGAIRFAAVQLGGAAALLDAMRDYLRGLDRTGDPYQRARAGQAAIAIETGNR
                     WLAGAARQVDLTLDASRSNDTTAAIAYANMTRSAIERVCLEVMRLVEHSVGARGLLRP
                     HPVERIVRDLTLYLRQPAPDAALAEAGAFALESDERPSRHWRDA"
     gene            complement(1541..>2459)
                     /locus_tag="VFV93_04255"
     CDS             complement(1541..>2459)
                     /locus_tag="VFV93_04255"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5164586.1"
                     /translation="GPVQGATHAVFRAGPVGMKLKSLLNGTPIRHRIHPVLVAVPIGA
                     WMMAAVLDAVEQRAGRREQRGLQAAADTSVAIGVVSALPTAISGLADWVDLYDHQRRV
                     GMAHALLNTVALACYGASLALRMGGGNRGLAKALSNAGFGVIAVSGSLGGELVYTLGV
                     NVPHTIYPRPPEDEVDVLASAELVEGTPVVVEVGRVPVLLVRREDTIYAVQEWCPHAG
                     GPLSEGTFDGDVVECPWHQSRFCLADGAPLQGPATVPLRTFEVREAGGRIYVRPSYEA
                     QSWPPAPAPIREQPQQIPLSANGESQLHA"
BASE COUNT          379 a          915 c          811 g          354 t
ORIGIN      
        1 cgaggtgctc gacgatcagc cgttgcgccc gcctgagatc gtcccagtcc cagtagtaac
       61 cgacctccga gacgaggatc agatcgaaca cttcctccgg gaattcggcc gggaccgcca
      121 tccgcgcgaa gcgaacctgt ggcaggtgac ggcagcgcgt gcgcgcctgc tccagcgccc
      181 gctcggcaac atcgaccgcc aatagccggt cgcaacgggc tgccagttgc tcggtcagga
      241 cgccgatcga gcagccgatc tcgaaggccg atctgtaccg ttcgcgcggc agggtggcca
      301 gcgtcgccgc gtacttgcgc gcttcgtagg gccgcgtagc gaagccccac ggatcgtcgt
      361 tctcggcata gacacgctcg aagtagccgg ccggcagcga gtcgtgctca cgcatcgcgc
      421 cagtgcctgc tgggtcgctc gtctgattcg agcgcgaagg cgcctgcctc cgccagggcc
      481 gcgtcggggg ctggctggcg caggtagagc gtcaggtcgc gcacgatccg ctcgaccggg
      541 tggggccgca gcaatccccg cgcaccgacc gagtgctcga ccagccgcat cacctccaga
      601 cagacccgct cgatcgccga tcgtgtcatg ttggcgtagg cgatggccgc tgtcgtgtca
      661 ttgctgcgcg aggcatcgag cgtcagatcg acctggcggg ccgcgccggc cagccagcgg
      721 ttgccggtct cgatagcgat cgcggcctga ccggcccgcg cgcgttggta cgggtcgccg
      781 gtacggtcca ggccgcgcag gtagtcgcgc atggcatcca gcagcgcggc ggccccgccg
      841 agctggacgg ccgcaaaccg gatcgcgccg cccgcgaacc acggctggcg gtgatagtcg
      901 cccggccggc cgatcaggtc gctcgcgttg acctcgacac cggagaagtc gacccggtag
      961 ctggccgagg cgcgcatccc gatcggctgc caccagtccg gatcgatctc gaccgcgacg
     1021 cgctcgatcg ggacgacgac catctgccag ccgccgtccg gcagcgctcc cggcacgatc
     1081 ggccgctgga catggccggc gccggaggcg aacgtcttcg cgcccgccag ccggtagcgg
     1141 ccaccagcca acggtgtgat gcggacgcca tcctgtgcct cggtgttcca gacgccgaac
     1201 aggcggccat cgcgggcatc ggccgctgtc cgtgtcagct gctcgaccgt cccgaacgtc
     1261 tggatgagct ggatggcgtt gacgtgtcct tcgtacaccc gcccaaccga gaggttgccg
     1321 cgcccgatct ccttcaacag cagcaacagt tccagcgcag tcgccggcga gccgtccagc
     1381 ccgcacccgc cgagatcgcg cggcaacggc gccaccagca ggccggcctc gcccatcagc
     1441 cggaactcct cgaccgggaa ggcccgctcg tcgtcgctgg cggcggcaag ctccgcgatc
     1501 tgatcggcga tccgggctgc cgtcgccagc agcgagccag tcatgcgtgc agctggctct
     1561 cgccgtttgc cgacagcggg atctgctgcg gctgctcgcg gatcggcgcc ggcgccggcg
     1621 gccaggattg cgcttcgtag ctgggccgga cgtagatgcg cccgcctgct tcccgaacct
     1681 cgaaggtgcg tagtggcact gtcgccggcc cctgcaacgg cgcgccatcg gccaggcaga
     1741 agcgcgactg gtgccacggg cactcgacga catcaccgtc gaacgtgccc tccgagagcg
     1801 gccctcccgc gtgcggacac cactcctgaa cggcgtagat cgtatcctcg cgccgcacta
     1861 gcaggaccgg cacccggccg acctcgacga cgaccggcgt tccctcgacc agctccgcgc
     1921 tggcgaggac atcgacctcg tcctcgggtg gccgtgggta gatcgtgtgc gggacgttga
     1981 cgcccagcgt gtagaccagc tccccaccca gcgagccact aacggcgatc acgccgaatc
     2041 cggcgttgga gagcgccttg gccagcccgc gatttccacc gcccatccgc agcgcgagtg
     2101 acgcgccgta gcaggccagt gcgaccgtgt tgagcagcgc gtgggccatc ccgacccggc
     2161 gctggtggtc gtagaggtcg acccagtcgg ccaggccgct gatcgccgtt ggcagagcgc
     2221 ttacgacacc gatcgcaacc gacgtatcag ccgctgcctg tagcccgcgc tgctcccgtc
     2281 gccctgcccg ctgctcgacc gcgtccagca cggccgccat catccaggcc ccgatcggaa
     2341 ccgcaaccag caccggatgg atccgatgcc ggatcggcgt cccgttcagc aaactcttca
     2401 gcttcatccc gaccgggccg gcccggaaga ccgcatgcgt cgccccctga accggcccc
//
LOCUS       DASUGJ010000195         5193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1137085, whole genome shotgun sequence.
ACCESSION   DASUGJ010000195 DASUGJ010000000
VERSION     DASUGJ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137085"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFV93_04260"
     CDS             complement(<1..552)
                     /locus_tag="VFV93_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164587.1"
                     /translation="MNKVLPRLSLVMVLMLLVSMLAACGGGGGDDDDKGDDDDAPTVT
                     ATSGSSQADASPTTGGSVTSPVPVAPTPTEAASPEPTATATQGASGEPVAVDALWFAS
                     DGQSVQGGTSRVVVSVNSKDDQDLRVGFFESEVGGSGPQWRAAGWMAVITASLLIGND
                     PSHYEFSFDVAGRIDGPSAGALMT"
     gene            1020..2948
                     /locus_tag="VFV93_04265"
     CDS             1020..2948
                     /locus_tag="VFV93_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5164588.1"
                     /translation="MPRPICFKRDADGYLPIEGYGAIGNGRTVALVGADGSIDWCPFP
                     RIDQPSVFARILDAHVGGYWQIVPDEPWTATRRYLDDTNILVTTYTTENGVVDLVDLM
                     PSIAFGSDLGLSNRLWDGMLLRRVLGVRGTVTMRQEIRPGFNYGREQTRVELIPGRGV
                     VLSGDSVALRVTYPAEMSAVDGGAVATWTVSEGDEGFTSASYHGLGAVVWADLPDRLA
                     RDLYEREVEGWQRWMTRCTYDGPYREFVRRSALALKLLDHLPSGAMAAAATTSLPEDL
                     GGVRNWDYRYAWIRDTSFAIHALSAIGFRDEAEGFLQWVIDATNNDPTSLQIMYRVDG
                     DSKLNEIELEHLEGYKGARPVRVGNGAHSQVQLDRFGEIVDAAWAHRRFGGAVNGALW
                     QYITEVVELIIERWRRVDSGIWEVRSEPRRMTYSNVMCWVGVARAIQLAKIDDRAAPL
                     DRWRAVRNEMHDEIMRLGVTVDGYFSQGFGDDILDAAALAFPTRSFIKVDHPVMRRTI
                     EAVERELTQDGLVGRYVVHKDRDNVDGLPGDEGHFLLCSCWLIDCLTGLGETDRATKL
                     LEKLLDRANDLGLYAEEVDADDGAFLGNFPQAFSHVGLINAIVNLARSQGDRARAPDD
                     DYGDISGAMRAGGRHDSK"
     gene            complement(3168..4064)
                     /locus_tag="VFV93_04270"
     CDS             complement(3168..4064)
                     /locus_tag="VFV93_04270"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEX5164589.1"
                     /translation="MTATSTPTPSPSPPATETPHPDPLVISELVLNDIDNTRLSQLSV
                     SEHPYFGGYTRVHGTLAISGTGNLTDVLLDVIESNAVVTTAGLTPTARDILIQPFGDD
                     GLIAITESTLLFEIPSVELSRIDQTSDGLVSLRVRAQSSSGADAVSSEWPVQKLVRYT
                     KANRYFVGEEAQGGNDWVRPSVRAYVEQLDSVLAGDFSNMNGGPFPPHFSHQTGKDID
                     LWFEGYNSLDTAAAQRLLAIVNQPAHVARIQNIFVAYERVAGNPFWEVLNGVVLADGR
                     PAASVIVPDPGHYDHFHLRVYY"
     gene            complement(4334..4762)
                     /locus_tag="VFV93_04275"
     CDS             complement(4334..4762)
                     /locus_tag="VFV93_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164590.1"
                     /translation="MRRVVPVAQQQVVDDVSLLIYSLEFYETGFVAVVRLSWIGDHGG
                     LPSIRWTAQDDRGTTFPYADMAGSGGGRHPDRFSWRIGCMFGAPVPEAATRLDLTATA
                     VGFLTFAGQDDGGRPRVERSQTIAGPWHFTIPLPTSQTGE"
     gene            complement(4819..>5193)
                     /locus_tag="VFV93_04280"
     CDS             complement(4819..>5193)
                     /locus_tag="VFV93_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164591.1"
                     /translation="ELYEGGFAAILLFEQVTEPPLPDRPDADGPILFRGMPAISASDD
                     HGNRYEGRMRAGYGGGGQTVSQMHEVCNFAPALNPDARTLTLDVSFVTRLGWNQAGKP
                     QWTSDDVLAGPWSATFSLPEGK"
BASE COUNT          885 a         1628 c         1751 g          929 t
ORIGIN      
        1 cgtcatcagc gcgccagccg acgggccgtc gattcgtccg gcgacgtcga acgagaactc
       61 atagtgcgac gggtcgttac cgatcagcag cgaggcggtg atgacggcca tccagccggc
      121 ggcgcgccac tgcgggccgg agccaccgac ttcggactcg aagaagccga cgcgcaggtc
      181 ctggtcatcc ttcgagttga ccgagacgac gacccggctg gtgccgccct ggacggactg
      241 gccgtcgctg gcgaaccaga gcgcgtcaac cgcgaccggc tcgccgctgg cgccctgggt
      301 ggcggtggcg gtcggttccg ggctggcggc ttccgtcggc gtcggggcga ccgggaccgg
      361 cgaggtgacc gagccaccgg tcgtcggcga ggcatcggcc tggctggagc cggaggtcgc
      421 ggttacggtc ggcgcatcgt cgtcatcgcc cttgtcgtcg tcatcaccgc cgccaccacc
      481 acaggcggcc agcatcgaga caagcagcat cagcaccatg accaacgaca ggcgtggcaa
      541 caccttgttc attgctctcc tcaatccgag cgagatgtat ccgtggttcg tgttggatgt
      601 ctgctgtcag tgtagcagtt ccaaccatca gtgcaatatc agcttcgcgc ataccctgcg
      661 cggatgtggt tcccccgaac gacctcctcc accacgtcac acgacccagc ctgcccctgc
      721 aggacaccat gtcgactgaa ccgcgccggc catccccgtc cgggccgatc caggctcctg
      781 cattacccag cgacgccgca gatagcgatg gcgtcacgcg ctcgtctatg cgatccgcat
      841 agctgaacgg tttgagcata cggagcgtta tgttcttgcg tatcgttcga caggtccaat
      901 cttaccctgt ccgttcggac agggtaaagg gggcgcgcac gtaacttgcg aaaatgtcgc
      961 gtcatgctca acgattggta gagccgacca atcgagcaca gcccgacgaa gggggatcga
     1021 tgccacgtcc aatctgcttc aagcgtgacg ctgatggcta cctgccgatt gagggctacg
     1081 gcgcgatcgg caatggccgg acggtggcgc tggtcggcgc ggatggctcg atcgactggt
     1141 gtccgttccc ccgcattgac cagccctcgg tcttcgcacg gatcctcgac gcgcacgtcg
     1201 gcggctactg gcagatcgtt cccgacgagc catggacagc gacgcggcgc tatctggacg
     1261 acaccaacat cctcgtcacg acctacacga ccgagaacgg cgtcgtcgat ctggtcgatc
     1321 tgatgccctc gatcgcgttt ggctccgacc ttggcctctc gaaccggctc tgggacggga
     1381 tgttgctacg gcgggtgttg ggcgtgcggg gaaccgtcac gatgcggcag gagatccggc
     1441 ccggcttcaa ctacggccgc gagcaaaccc gcgtcgagct gatccccggt cggggcgtcg
     1501 tgctctccgg cgactcggtc gcgctgcggg tgacgtatcc ggcggagatg tcggcggttg
     1561 acggtggcgc tgtcgcgacc tggacggtga gcgagggcga cgagggcttc acctcggcca
     1621 gctaccacgg gcttggcgcg gttgtctggg ccgacctgcc cgaccggctg gcgcgcgacc
     1681 tctacgagcg cgaggtcgag ggctggcaac gctggatgac gcgctgtacc tatgatggac
     1741 cgtaccggga gttcgtccgc cgcagcgcgc tggccctcaa gctgctcgat cacctgccga
     1801 gcggcgcgat ggcggccgcc gcgacgacct cgctgcccga ggatctgggc ggtgtgcgca
     1861 actgggatta ccgctacgcc tggatccgcg acacgagctt cgcgatccac gcgctgagcg
     1921 cgatcggctt ccgcgacgag gccgaaggct tcctgcaatg ggtgatcgac gcgaccaaca
     1981 acgatccgac gtcgctccag atcatgtacc gcgtcgatgg tgacagcaag ctgaacgaga
     2041 tcgagctgga gcacctcgaa ggctacaagg gcgcccgtcc ggtccgggtc ggcaacggtg
     2101 ctcacagcca ggtccagctc gatcgcttcg gcgagatcgt cgacgccgcc tgggcgcatc
     2161 gccgtttcgg cggggccgtc aacggcgcgc tctggcagta catcaccgag gtcgtcgagt
     2221 tgatcatcga acgctggcgg cgggtcgatt ccggcatctg ggaggtgcgc tccgagccgc
     2281 gacggatgac ctattccaac gtcatgtgct gggtcggcgt ggcgcgtgcg atccagttgg
     2341 ccaagatcga cgaccgcgcc gcgccgctgg atcgctggcg ggccgtccgc aatgagatgc
     2401 acgacgagat catgcggctg ggtgtcacgg tcgacggcta cttcagccag ggattcggcg
     2461 atgacatcct cgatgccgcc gcgctggcct tcccgacgcg cagcttcatc aaggtcgatc
     2521 accctgtcat gcggcgaacg atcgaggccg tcgagcgcga gctgacgcag gacggtctgg
     2581 tcggccgcta cgtcgtccac aaggacaggg acaacgtcga tggccttccg ggtgacgagg
     2641 gacatttcct gctctgctcc tgctggctga tcgactgcct gaccggcctt ggcgagaccg
     2701 accgcgcgac caaactgctg gagaagctgc tcgaccgcgc caacgacctc ggcctctacg
     2761 ccgaggaggt cgacgccgac gacggtgcct tcctcggcaa cttcccgcag gcgttctcgc
     2821 acgtcggctt gatcaacgcg atcgtcaacc tggcccgcag ccagggcgac cgcgcccggg
     2881 ccccggacga cgactacggc gacatcagcg gcgcaatgcg ggccggcggg aggcacgatt
     2941 cgaagtagcc actgcctgta cgatctcgcg tcagatccat aacaattcaa taatcaatcc
     3001 ggtacgggcg atatcgcaat atcgccccct gctccccacc caagatgttc gggcgggaca
     3061 cgaagggcga tattgcgata tcgcccgtac cggattggga gattgggttt gcctttattg
     3121 tgaaagcgag cccgtctggg actcccaaag ttgctgcagt gtcccaatca atagtagacg
     3181 cgcaaatgga agtggtcgta gtggcccgga tcagggacga tcacgctggc tgccggccgg
     3241 ccgtcggcga ggacgacgcc gttgagtacc tcccagaacg ggttacctgc gacgcgttcg
     3301 taggcgacga agatgttctg aatgcgggca acgtgggccg gctggttgac gattgccagt
     3361 agccgctggg cagcggcggt gtcgaggctg ttgtagccct cgaaccagag gtcgatgtcc
     3421 ttgcccgttt gatgactgaa gtgcggcgga aagggtccgc cgttcatgtt gctgaaatca
     3481 ccggccagca cgctgtccag ctgctcgaca tacgcgcgga cgctcggacg tacccagtcg
     3541 ttgccgccct gcgcttcctc cccaacgaag tagcggttgg ccttggtgta gcgcacgagc
     3601 ttctggacag gccactcgct gctcaccgcg tccgcgccac tgctcgactg ggcgcggacg
     3661 cgcaatgaga cgaggccatc cgatgtttga tcgatccggc tcagttccac tgatggaatc
     3721 tcaaacagca gggtgctctc ggtgatggcg ataagtccgt cgtctccgaa tggctggatg
     3781 aggatgtcgc gtgccgttgg tgtgagaccg gccgtcgtga cgaccgcgtt ggattcgatg
     3841 acgtcgagca gaacgtcggt gaggttgccg gtccccgaga tggccagcgt tccgtgcacg
     3901 cgggtgtagc cgccgaagta ggggtgctcg ctgacgctca gttggctcag gcgggtgttg
     3961 tcgatgtcgt tgaggaccag ttcgctgatg accagcggat cgggatgcgg cgtctccgtt
     4021 gcgggaggac tcggtgatgg agtcggcgtc gatgttgccg tcatcgttgt cgttgcggtc
     4081 agcgtggccg tcgctgtcgg cgccaaggtg gcggttggtg gggcgggtgt gctggcgtgt
     4141 gtcgttgggg tcgtgttgat gatcggtgac ggcgtagacg gtgggtcgga ccagcaggct
     4201 gcggcgacga caacgatgaa actggcggat acaatgtatc caaaaggttc tcgtatacga
     4261 catactgggc caaacgtggc gacagcccgg acgagggtgt cgccaaatcc tctccagcct
     4321 tgccgctttg gcgtcattct ccggtctgtg aagtggggag cgggatcgtg aagtgccacg
     4381 gccccgcgat cgtttgtgag cgttcgacgc gcggtcgacc gccgtcgtcc tgaccggcaa
     4441 acgtcaggaa tccgacggcg gttgcagtca ggtccagccg cgtcgccgct tctgggaccg
     4501 gcgcgccgaa catgcagccg atccgccatg agaagcggtc gggatggcgc ccgccaccgc
     4561 tgccggccat gtccgcgtac gggaacgtcg tgcctcggtc gtcctgggcg gtccatctga
     4621 tgctcggtag cccgccatga tcgccgatcc agctgaggcg gacgacggcg acaaaaccag
     4681 tctcatagaa ttccagcgag tagatgagca gtgaaacatc gtcgacgacc tgctgctggg
     4741 cgaccgggac aacgcgccgc aagttatgcg accccatcga cggggcgacc tggcagctcg
     4801 gtggctcggg atggtcggtc atttgccctc cggcaggcta aaggtagcgc tccacggacc
     4861 tgcgagaacg tcgtcagaag tccactgcgg cttgccggcc tggttccagc cgaggcgggt
     4921 gacgaagctg acatcaagcg tcaaggtccg cgcgtcggga ttgagggcgg gcgcgaagtt
     4981 acagacctcg tgcatctgtg acacggtctg gccgccgccg ccatagccgg cgcgcattct
     5041 gccctcgtag cgattgccgt ggtcgtcgct ggcgctgatg gccggcatcc cgcgaaagag
     5101 aatcgggccg tcggcgtcgg gccggtcggg gagcggcggc tcagtcacct gctcgaacag
     5161 caggatggct gcaaacccgc cctcgtacag ctc
//
LOCUS       DASUGJ010000196         8158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_444388, whole genome shotgun sequence.
ACCESSION   DASUGJ010000196 DASUGJ010000000
VERSION     DASUGJ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444388"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="VFV93_04285"
     CDS             <1..1752
                     /locus_tag="VFV93_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5164592.1"
                     /translation="AIVGGSLLAACGGDDDDDDDGGETPAASGETPAAGGGATATTAS
                     SGGSSSPSTGSPEAEATSSGGSAPVGMTPVAELADEQVMRLPSGEPNTMDPGVSFGGD
                     ELDIMFNIFDGVTGVDQQTGEIVPRVAETYEGNADGSEYTFNLRTGLQWSDGTPMNAN
                     DFVYSWKRVLDPNTKSQYMPALYSIKGAQEAAESGAGFEDIGVEAVDDNTLKVTLVGP
                     TPYFPLLTTTWTFYPVPKHIIDDKGDAWVEAENIVSNGPFILTSWDHNQEMILEQNPN
                     FYGDTPTLTRAEYRLFDDPVSQAYVSFENDELDYAQVTGADVDRAMGDADASKNLLTF
                     SLSNCNFVVCDTSNPPTDQKPFRQALSMAISRETLTGQVLKGQFDPAYTILAPDIKGN
                     NPDASIGEDVDKAKSLLTDAGIDDPGSVEIELVYISTPARNKTVAEYLQATWNKNLGI
                     EVKLTPIESTAYSDWRASRETQKYNTYTGSWGSDFSDASNWFNQNFTHAADHYRNHYN
                     NPDFDSLVEEAAVNTDEAEREQQYKDAEVIIVDDAPIIPLYRSKAFRAIKPYVKNLFL
                     QPILSVVHLRTVQIEKH"
     gene            1828..2754
                     /locus_tag="VFV93_04290"
     CDS             1828..2754
                     /locus_tag="VFV93_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164593.1"
                     /translation="MQQRAYIIRRVLLLFPTLLAIYTLTFLLIHSTPGGPWSQGEKPV
                     PPIVLEQLNKAYGLDQPLWKQYVNYLGNVLQGDFGPSFSQRSRTVSDILKDTFPVSLK
                     LGLVAMGIALITGVTLGVIGAVRHNTIIDYISSFAAIVGISTPSYVTCSLLVLIFASQ
                     LHLVPTGGWDGIFSKTIFIPAFALALFPAAVLARYTRSAMLDVLSSDYVRTARAKGLR
                     ESAVIIRHCIRNAMLPVLTVAGVVLADVITGSFFVETIYSVPGIGRYFVESISKRDYP
                     VILGTVLLFGAVISIMNLIVDLLYPLLDPRIE"
     gene            2789..3766
                     /locus_tag="VFV93_04295"
     CDS             2789..3766
                     /locus_tag="VFV93_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164594.1"
                     /translation="MRSAAEPADSLRTAGAASFNGQQRLNSLWSDAFRRLRRNRLAFA
                     SSIFIIVLLLLAIVFPVLSSTTYSDQNYDAITQSPSRDHLMGTDQLGRDILVRLIFGA
                     RISLAVGIIVQVVILLIGVPAGLLAGYFGGTLDTIIMRIVDVLYAMPNLLFVILIMTF
                     LRGQLGAEHSGFLSTVAEADTRLGGLVGVFIGLGLISWLTVARIVRAQTMSLKQKEFV
                     EAARGLGASHRQIMFRHLLPNSMAPIVVATTLGIPAAILYEAGISFIGLGVIPPMPSW
                     GLMISEAIPNLRAYPYMLIFPAAALSLTVLAFNFLGDGLRDALDPRMKR"
     gene            complement(3851..>8158)
                     /locus_tag="VFV93_04300"
     CDS             complement(3851..>8158)
                     /locus_tag="VFV93_04300"
                     /inference="COORDINATES: protein motif:HMM:NF015387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEX5164595.1"
                     /translation="VRNYLSIDGFHVFSGGNEFRGLAFTHLRRSIWLSGHNAHDNIVA
                     GCFIGTDPAGQPWYEAINNVEGRGSDDGAFGMWIDNGAHHNRIGGTGPADRNVISGNA
                     NDGGGGSDTGTEYNRFIGNLIGLSPDGTRRLRNMSDGLDLNFGMSHTQVGGTAPGERN
                     VIAGNSGEGVEISHGPQTGYNVVAGNYFGVAPDGNTVVNNVHFNSGYSVSIEDSTSNN
                     TIGPGNVMVNSWKGGIHIYGALGSGNHIFGNLIGITPNGTANSLTANRGEGIRIRFGA
                     SDVTIGPDNVITNNTRAGVLVIDNSSQFNTITQNSIYNNGEIGIDLGGNKDTGGDGVT
                     PNDGSWGSGPNQRIDYPVITSATTTSVSGTALTGYRVEIFIADSPAGQYGEGRTFVGA
                     GVANGSGHFTIPVSGVAAGNVLTATSTDSAGNTSEFSVNVAANSGSTNTPPTASITAP
                     AAGSTVNGTVSIAVNANDAEDGVGSLDVDVRIDGGAWQAASWNGGTGRYQYSWNTQAA
                     SNGNYTIEARATDSDAAVATTGPINVSVNNVVDQVYSLPALIQAEDYDSYHDTTSGNS
                     GGRYRSDDVDIETCTDPTTPTGLTCYDVGWTAAGEWLAYRVNVPTAGNYAFALRVATP
                     SSNRTIQLQLDGVNLGAPVAIPDTDGYQNWATATAVRQLPAGQHELRLVFGSTNVNLN
                     SIDITAAANTPPAVAIVTPANGATVNGTTTVALNASDAEDATGTLSVQVRIDSGIWQP
                     AAWNAGTGRYQLSWDTTTASNGNHTIYARATDSSSAGAIEDAVTVNVNNVVAPTFTLP
                     TLIQAEDYTNSHDTTSGNSGGRYRFEDVDIETCTDATTPAGQTCYSIGWTDTGEWLAY
                     DIRVPSAGNYVFALRVSTPSSNRTIQLQLDGVNLGAPVAIPDTDGYQNWTTATAVRSL
                     PAGDHELRLVFGSTNVNLNSVNVTASSNTAPSVGIATPASGATVSGTTTIGVTASDAQ
                     DAAGTLDVAVRIDGGVWQAASWNAGTGRYQYAWNTASLSNGSHTIEARATDSNGADAT
                     DGPRTVSVNNAPVADFVLPTLIQAEDYDSYSDTTSGNTGGRYRFNDVDIQTCTDATTP
                     AGQTCYNVGWTDPGEWLGFDIRVPSAGTYTFAVRVATPSNNRSVQLQLDGANLGAAIS
                     VPNTGGYQTWTTVTAARQLPAGDHQLRLVVSGTNMNVNYVDVTAGGVANRAPAVAIVT
                     PTSGATVSGATTVGLVANDTEDAAGTLDVQARIDDGAWQAAHWNDTSSRYQFSWDTST
                     ASNGGHTIAARATDSDGATGTASPITISVSNQTLVTLPATIQAEDYVDYFDSDAGNVG
                     GVYRNDDVDLGSCSDGSGCVYVGWTMAGEWLAYNVSTSSARDWTFRLRYASPSSGRRV
                     KVEVDGQIISTVSLPASGGWDTWRDAAVGTTTLSAGQHTVRLLIDLDGINVNWMSVS"
BASE COUNT         1290 a         2651 c         2736 g         1481 t
ORIGIN      
        1 gcgatcgtcg gcggctcgct gctggcggcc tgcggtggcg acgatgacga tgatgacgac
       61 ggcggagaga caccggccgc cagcggagag acgccagcag cgggcggcgg agcgaccgcc
      121 acgaccgcca gttccggcgg cagctccagc ccatcgaccg gcagcccgga ggccgaagcg
      181 acctccagcg gtggcagcgc gccggtcggg atgacgccgg tcgcggagct ggccgacgag
      241 caagtcatgc gactgccgag cggcgagccg aacacgatgg acccgggcgt cagcttcggc
      301 ggcgacgagc tggacatcat gttcaacatc ttcgacggcg tcaccggcgt cgaccagcag
      361 accggcgaga tcgtgccgcg cgttgccgag acctacgagg gcaacgccga tggttccgaa
      421 tacacgttca acctgcggac cggcctacaa tggtccgacg ggacgccgat gaacgccaac
      481 gacttcgtct acagctggaa gcgggtgctg gacccgaaca ccaagtcgca atacatgccg
      541 gctctctact cgatcaaggg cgcccaggag gcggccgaga gtggagccgg gtttgaggat
      601 atcggcgtcg aagcggtcga tgacaacacg ctcaaggtga cgctggtcgg cccgacgccg
      661 tacttcccgc tgctgacgac gacctggacg ttctacccgg tcccgaagca catcatcgat
      721 gacaagggcg acgcctgggt cgaggccgag aatatcgtct cgaacggacc gttcatcctg
      781 acatcctggg accacaacca ggagatgatc ctggagcaga accccaactt ctacggcgac
      841 acaccgacgc tgacccgcgc cgagtatcgg ctgttcgacg acccggtctc ccaggcctac
      901 gtctcctttg agaacgacga gctggactac gcgcaggtaa ccggcgccga tgtcgatcgg
      961 gcgatgggcg acgctgatgc gagcaagaac ctgctgacct tctccctctc caactgcaac
     1021 ttcgtcgtct gcgacaccag caacccgccg accgaccaga agccgttccg ccaggcgctc
     1081 tcgatggcga tctcgcgcga gacgctgacc ggccaggtcc tcaagggcca gttcgacccg
     1141 gcctacacga tcctcgcgcc ggacatcaag ggcaacaacc cggacgcgtc gatcggcgag
     1201 gatgtcgaca aggctaagag cctgctgacc gatgccggca tcgacgaccc cggctcggtc
     1261 gagatcgagc tggtctatat cagcacgccg gcccgcaaca agacggtggc cgagtatctg
     1321 caggcgacct ggaataagaa cctggggatc gaggtcaagc tgaccccaat cgagtcgacc
     1381 gcctactccg actggcgcgc ctcccgcgag acgcagaagt acaacaccta caccggctcg
     1441 tggggctccg acttcagcga cgcgtcgaac tggttcaacc agaacttcac ccatgcggcc
     1501 gaccactacc gcaaccacta caacaacccg gacttcgact cgctggtcga ggaagccgcc
     1561 gtcaacaccg acgaggccga gcgggagcag cagtacaagg acgccgaggt gatcatcgtc
     1621 gatgacgccc cgatcatccc gctctaccgc tcgaaggcat tccgggcgat caagccgtac
     1681 gtgaagaacc tgttcctgca gccgattctc tcggtcgtcc acctgcggac ggtccagatc
     1741 gagaagcact aagagcgatg ctggcgcggc cggggtaaca attccccggc cgcgctgccg
     1801 taccgcaatg aaacgagacg agcgagcatg caacaacgtg cctacatcat tcgccgggtg
     1861 ctgctgctct tcccgacgct gctggcgatc tatacgctga cgttcctgct gatccactcg
     1921 acgccgggcg ggccgtggtc gcagggcgag aagccggtgc cgccgatcgt gctggagcag
     1981 ctcaacaagg cgtacggcct cgaccagcca ctctggaagc aatatgtcaa ctacctcggc
     2041 aatgtcctcc agggcgactt cgggccgtcc ttcagccagc gctcgcggac agtctccgac
     2101 atcctgaagg acacgttccc ggtctcgctc aagctcgggc tggtcgcgat gggaatcgcc
     2161 ttgatcaccg gcgtcacgct cggtgtaatc ggggctgtcc ggcacaacac gatcatcgac
     2221 tacatctcca gcttcgccgc gatcgtcggc atctcgacgc cctcgtacgt cacctgctcg
     2281 ctgctggtgc tgatcttcgc cagccagctg catctcgtgc cgaccggcgg ctgggacggg
     2341 atcttcagca agacgatctt catccccgcc ttcgcgctgg cgctcttccc ggcggccgtc
     2401 ctggcgcgct atacccgctc ggcgatgctg gacgtcctct cgtccgacta cgtccggacg
     2461 gcgcgggcca aggggctgcg cgagagcgcc gtcatcatcc gccactgcat ccgtaacgcg
     2521 atgctgccgg tcctgacggt ggccggcgtc gtcctggccg atgtcatcac cggctcattc
     2581 ttcgtcgaga cgatctactc ggtacccggc atcgggcgct acttcgtcga gagcatctcg
     2641 aagcgcgatt acccggtcat cctcgggacg gtcctgctgt tcggcgcggt catttcgatc
     2701 atgaacctga ttgtcgatct actctaccca ctgctcgacc cgcgtatcga gtgatgccgg
     2761 gcagcgggca gcgacaggag caactggtat gcggtcagcg gcggagcctg ccgactctct
     2821 gaggacagcc ggagcggcga gtttcaacgg ccagcaacgg ctcaacagcc tctggagcga
     2881 cgccttccgg cggctgcgcc ggaacaggct ggcgttcgcg tcatcgatct tcatcatcgt
     2941 cctgctgctg ctggcgatcg tcttcccagt cctctcctcg accacctact cggaccagaa
     3001 ctacgacgcg atcacccaat cgcccagccg cgaccacctg atggggaccg accagctcgg
     3061 gcgggacatc ctcgtccggc tcatcttcgg cgccagaatc tcgctggcgg tcgggatcat
     3121 cgtccaggtc gtgatcctgc tgatcggcgt cccggccggg ctgctggccg gctacttcgg
     3181 cggcacgctg gacacgatca tcatgcggat cgtcgatgtg ctctacgcga tgccgaacct
     3241 gctgttcgtg atcctgatca tgaccttcct gcgcggccag ctcggcgcgg agcattcggg
     3301 gttcctcagc accgtcgccg aagcagacac gcggttgggc gggctggtcg gtgtcttcat
     3361 cggactgggg ttgatcagct ggctgacggt cgcgcggatc gtccgggcgc agacgatgtc
     3421 gctcaagcag aaggagttcg tcgaggcggc ccgtggcctg ggcgccagcc accggcagat
     3481 catgttccgc cacctgctgc ccaacagcat ggcgccgatc gtggtcgcga caacgctcgg
     3541 catcccggcc gcgatcctct acgaggccgg catcagcttc atcgggctgg gcgtcatccc
     3601 gccgatgccg agttgggggc tgatgatcag cgaggcgatc cccaacctgc gcgcctaccc
     3661 ctacatgctg atcttcccgg ccgccgccct ctcactgacg gtcctcgcct tcaacttcct
     3721 cggcgatggc ctccgcgacg cccttgatcc gaggatgaag cggtgacggg gcgcgggaat
     3781 cgggatccgg ggctgggaca cttgcaaatt ctggtcgaaa ccgatccgtc acccagcccc
     3841 gaatcccgcg ttacgaaacc gacatccagt tcacattgat cccatcaaga tcaatgagca
     3901 ggcgaaccgt gtgctggccg gcgctgagcg tggttgtgcc gacggcggcg tcgcgccagg
     3961 tgtcccagcc gccgctggct gggaggctga cggttgagat gatctggcca tcgacctcga
     4021 ccttgacgcg gcgtccggag gatggcgagg cgtagcgcag ccggaaggtc cagtcccgtg
     4081 cgctgctggt gctgacgttg taggccagcc actcaccggc catcgtccag ccgacgtaaa
     4141 cgcagccgct accgtccgag caggagccga gatcgacatc gtcgttgcgg tagacgccgc
     4201 cgacgttgcc ggcgtccgag tcgaagtagt cgacgtagtc ctcggcctgg atcgtggccg
     4261 gcagtgtcac caacgtctgg ttgctgacgc tgatggtgat cgggctggcc gtgccggtcg
     4321 cgccgtcact gtcggtggcg cgggccgcga tcgtgtggcc gccgttgctg gccgtcgacg
     4381 tgtcccagct gaactggtag cggctggatg tgtcattcca gtgggccgcc tgccaggcgc
     4441 cgtcgtcgat ccgcgcctgg acatcgagcg tcccggccgc gtcctcggtg tcattggcga
     4501 ccaggccgac cgtcgtcgcc cctgagacgg tcgcgccgga ggtgggcgtc acgatcgcga
     4561 ccgccggagc gcggttggcg acgccaccag cggtgacgtc cacgtagttg acgttcatgt
     4621 tggtcccgct gacgaccagc cgcagctggt ggtcaccggc cggcagctgg cgggcggccg
     4681 tcaccgtcgt ccaggtctgg tagccgccgg tattgggaac gctgatggcc gcgcccaggt
     4741 tggcgccgtc gagctggagt tgaacgctgc ggttgttgct gggcgtcgcg acccggacgg
     4801 cgaaggtgta ggtgccggcc gacggaacgc gaatgtcgaa gccgagccac tcacctggat
     4861 cggtccagcc gacgttgtag caggtctgac cggccggcgt cgtcgcatcc gtgcaggtct
     4921 ggatgtcaac gtcgttgaag cggtagcgtc cgccggtgtt gccactggtc gtgtcggagt
     4981 agctgtcgta atcctcggct tggatcagcg ttggcaggac gaagtccgcc acgggcgcat
     5041 tgttgacgct gacggtccgt gggccgtcag tcgcgtcggc gccattgctg tccgtcgctc
     5101 gggcctcgat cgtgtggctg ccattgctga ggctggccgt gttccaggcg tactggtagc
     5161 ggccggtgcc ggcgttccaa ctagccgcct gccagacgcc gccgtcgatg cggaccgcca
     5221 catcgagcgt cccggccgcg tcctgggcgt cgctggccgt cacgccgatc gtggtcgtgc
     5281 cggagacggt cgcgccgctg gccggcgtcg cgatgccgac tgacggtgct gtgttcgacg
     5341 acgccgtgac gttcacagag ttcaggttca cgttggttga gccgaagacg agccgtagtt
     5401 cgtggtcacc ggccgggagc gagcgaactg ctgtcgccgt cgtccagttc tggtagccgt
     5461 ccgtgtccgg aatggcgacc ggtgcgccca gattcacgcc gtccagctgg agctggatcg
     5521 tccggttgct gctgggggtc gagacccgta gggcgaagac gtagttcccg gctgacggga
     5581 cgcggatatc gtaggccagc cactcgccgg tgtcggtcca gccgatgctg tagcaggtct
     5641 ggccggctgg tgtggtcgca tcggtgcagg tctcgatatc gacatcttcg aaccggtagc
     5701 ggccaccgga gttgccgctg gtcgtgtcgt ggctgttggt gtagtcctcg gcctggatca
     5761 gcgttggcag cgtgaaggtt ggcgcgacga cgttgttgac gttaaccgtc accgcgtctt
     5821 cgattgcgcc ggccgagctg ctgtcggtgg cccgcgcgta gatcgtgtgg ttaccgttgc
     5881 tggccgtcgt cgtgtcccaa ctcagctggt agcggcccgt cccggcgttc caggcggccg
     5941 gctgccagat gccgctgtcg atccggacct gaacgctgag tgtcccggtc gcgtcctcgg
     6001 cgtcactggc gttgagggca accgtcgtcg tgccgttgac ggtcgcgccg ttggccggcg
     6061 tcacgattgc gacggccggc ggcgtgttgg cagcagcggt gatgtcgatc gagttgaggt
     6121 tgacattcgt tgagccgaag accaaccgta gctcgtgctg gcctgccggg agctggcgga
     6181 cggctgtggc ggtggcccag ttctggtagc catccgtgtc cgggatggct accggcgcgc
     6241 cgaggttgac gccgtcgagc tgaagctgga tcgtccggtt gctgctcggc gtcgcgacgc
     6301 gcaacgcgaa ggcgtagttg ccggccgtcg gaacgttgac gcggtaggcc agccactcac
     6361 cggcggccgt ccagccgacg tcgtagcagg tcaggccggt cggcgtcgtc gggtcggtgc
     6421 aggtctcgat atcgacgtca tcgctgcggt agcgcccgcc gctgttgccg ctggtcgtgt
     6481 cgtgatagct gtcgtagtcc tcggcctgga tcagggccgg gagggagtag acctgatcga
     6541 cgacgttgtt gactgagacg ttgatcgggc cggttgtcgc gacggcggcg tcgctgtcgg
     6601 tcgcccgcgc ctcgatcgtg tagttgccgt tgctcgcggc ctgcgtgttc cagctgtact
     6661 ggtagcggcc agtgccgccg ttccagctgg cggcctgcca ggcgccgccg tcgatacgga
     6721 catcgacatc gagcgagccg acgccatcct cggcgtcgtt ggcgttgacg gcgatcgaca
     6781 ccgtcccgtt gacggtcgaa ccagcggccg gggccgtgat gctggccgtc ggcggcgtgt
     6841 tggttgagcc actgttcgcg gcgacgttga ccgagaactc ggacgtgttg ccggccgagt
     6901 cggtcgaggt cgccgtcagc acgttgccgg ccgcgacgcc gctgaccggg atcgtgaagt
     6961 ggccgctacc gttggcgaca ccagcgccga cgaaggtgcg gccctcgcca tactggccgg
     7021 ccggggaatc ggcgatgaag atctcgaccc ggtagccggt cagcgccgtc ccgctgacgc
     7081 tggtcgtcgt cgccgacgtg atgaccgggt agtcgatgcg ctggttcggg ccgctacccc
     7141 aactgccgtc gttgggcgtg acgccgtcgc cgccggtgtc cttgttcccg ccgaggtcga
     7201 tcccgatctc gccattgttg tagatcgagt tctgggtgat cgtgttgaac tgcgacgagt
     7261 tgtcgatcac cagcacaccg gcgcgggtgt tgttggtgat gacgttgtcc ggcccgatcg
     7321 tcacgtccga cgcgccgaag cggatgcgga tcccctcgcc acggttggcg gtcagcgagt
     7381 tggcggtccc gttcggggtg atcccgatca ggttcccgaa gatatggttg ccggagccga
     7441 gcgcaccgta aatgtggata ccgcccttcc agctgttgac catcacattg ccggggccaa
     7501 tcgtgttgtt ggaggtgctg tcctcgatcg agaccgagta gccggagttg aagtgcacgt
     7561 tgttgacgac ggtgttgccg tccggcgcaa cgccgaagta gttgccggcg acgacgttgt
     7621 agccggtctg cgggccgtga gagatctcga caccctcacc ggagttgccg gcgatgacgt
     7681 tgcgctcgcc gggcgccgtg ccaccgacct gggtatgcga catcccgaag ttgagatcca
     7741 gcccatcgct catgttgcgc agacggcggg tgccgtccgg gctgagaccg atcagattgc
     7801 cgatgaaccg gttgtactcg gttccggtgt cagagccgcc gccgccgtcg ttggcgttgc
     7861 cggagatcac gttgcgatcg gccggaccgg tgccgccaat tcggttgtga tgcgcgccgt
     7921 tgtcgatcca catcccgaac gcgccatcat cgctgccgcg cccctcgaca ttgttgatcg
     7981 cctcgtacca cggctgcccg gccgggtcgg tcccgatgaa gcagccggcg acgatgttgt
     8041 cgtgcgcgtt gtggccgctg agccagatcg agcggcgcag gtgggtgaac gccagcccgc
     8101 ggaactcgtt gccgcccgag aagacgtgga agccgtcgat actgaggtag ttgcggac
//
LOCUS       DASUGJ010000197         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_444406, whole genome shotgun sequence.
ACCESSION   DASUGJ010000197 DASUGJ010000000
VERSION     DASUGJ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444406"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1166
                     /locus_tag="VFV93_04305"
     CDS             243..1166
                     /locus_tag="VFV93_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5164596.1"
                     /translation="MDDRTQRIVDALVTCGQRVREVVVRAEYHRLAEVVGLGADGADT
                     THIDQVAEDIALACLEASEPRMNILSEEIGFLDRGSSLTAIVDPIDSTNNAIAVPNFV
                     RQPGASLAELAETPLREGHVFGFPYYAFSVGVVEDGELIAGCVMNLPTGEVFTARRGH
                     GVELDGVPVRAREVTTLAEARVALIRPETDAAWRTLRTITVGARRIRLTGCSALDLAL
                     IACGTLEALVNPNRISPKGYGEKIVDYAGALALLNETGSVLTGFDGVPVTLDLDLTRR
                     TPLVAAATPALHADLIEALHAESWDDVLRGE"
     gene            1238..1573
                     /locus_tag="VFV93_04310"
     CDS             1238..1573
                     /locus_tag="VFV93_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HEX5164597.1"
                     /translation="MTDIETQSDQFELYDLRVTVHAIEGRSVCGMQIGDYFELTESSR
                     VRIPEGKHFCLYALQSVIPLLTPKQRQLDANDWLERDTLVACPDPEERLIMKIQRVRR
                     RTLATDDLT"
     gene            1570..2568
                     /locus_tag="VFV93_04315"
     CDS             1570..2568
                     /locus_tag="VFV93_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5164598.1"
                     /translation="MTGRELSIAFQSDKPPGDYAELARLVEGYGFDTLSIYADLLFQP
                     PVVPLAVAAQATSRVRLGPASLNPFTLHPVEIAGQIAALDLLSGGRAYLGLSRGAWLE
                     DIGVGQERAVSRMREAIDVIEHLLAGRLEPYEGQHLRLSEHHRLHYTVQRERVPLVVG
                     SWGRQTLRLAGQRADEVKVGGSANPDVVPIVRDWIAEGATSVGRDPDSIGICLGAVTV
                     VDEDRDVARALIRREMALYLPVVAGLDPTVSVDPDLLARIEALVARGEAASAGDLIPD
                     DLLDRFAFAGNPDDIIRQSEALLAAAVTRIEFGTPHGVTPARGLKLLGERVLPALR"
     gene            complement(2578..>2835)
                     /locus_tag="VFV93_04320"
     CDS             complement(2578..>2835)
                     /locus_tag="VFV93_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164599.1"
                     /translation="LFLAAGIYLWLRVRASYGALVIAPSLLFGLSGNPQSLNRYLLVL
                     FPVYILLAQIRSEAIRLAIIIAFGLGLAIEGFFFMNEMWAG"
BASE COUNT          463 a          906 c          996 g          470 t
ORIGIN      
        1 agccacttct tcgggcgtga agtagcgttt ccagtagtcg gttgcgagcc ctgcgtcgtg
       61 gcgcgccatt ggcattctcc aggctattgc gaattggcaa ggactggatg cggcttcagg
      121 tggttggttc gcgtatcctc actgtagtgc tgttgccgtc gcccgtcaac ggaagcggca
      181 ggcggggaat gccaccgacc aacaatccgg ccgaatcgca aaggttgacg aactcaatgc
      241 agatggatga caggacgcag cggatcgtgg atgcgttggt gacgtgcggc cagcgcgtgc
      301 gcgaggtcgt cgttcgcgcc gaataccacc ggctggccga ggtcgtcggg ctgggcgccg
      361 atggcgcgga cacgacccac atcgaccagg tcgccgagga catcgccctg gcctgcctcg
      421 aagcgagcga gccgcggatg aacatcctct cggaggagat cggcttcctc gatcgcggct
      481 cgtcgctgac ggcgatcgtc gacccgatcg actcgaccaa caacgcgatc gccgtcccca
      541 acttcgtccg ccagcccgga gccagcctgg ccgagctggc cgagacgccg ttgcgcgagg
      601 ggcacgtctt cgggtttccc tattacgcct tctcggtcgg tgtcgtcgag gacggcgaac
      661 tgatagccgg ctgcgtcatg aacctgccga ccggcgaggt cttcacggcc cggcgtgggc
      721 atggtgtcga gctcgatggc gtcccggttc gcgcccgcga ggtgacgacg ctggccgaag
      781 cgcgagtcgc gctgatccgg ccggagacgg acgcggcctg gcggacgctg cggacgatca
      841 ccgtcggtgc ccggcgcatc cgcctgaccg gttgcagcgc gctcgacctg gcactgatcg
      901 cctgcgggac attggaggca ctggtcaacc cgaaccgcat ctcaccaaag ggctacggcg
      961 agaagatcgt cgactatgcc ggtgcgctgg cgctcctcaa cgagaccggc agcgtcctga
     1021 ccggcttcga cggcgtcccg gtcacgctcg acctcgacct cacccgacgc accccgctgg
     1081 tcgctgccgc gacaccggcc ctgcacgccg acctgattga agcgctccat gccgagtcgt
     1141 gggacgatgt gctgcgagga gagtgatgcg tgatgcgtga cgcggattgg ttcgggcctt
     1201 cgcatcacgc atcacgtcca gacattggag catcgttgtg accgatatcg aaacgcaatc
     1261 cgaccagttc gagctctacg acctgcgcgt caccgtccac gcgatcgagg ggcggtcggt
     1321 ctgcgggatg cagatcggtg attacttcga gctgaccgag tcgagccggg tgcggatccc
     1381 ggaggggaag cacttctgcc tgtacgcgtt gcagtcggtc attccgctgc tgacgccaaa
     1441 gcagcgccag ctcgacgcca acgactggct ggagcgggac acgctggtcg cctgcccgga
     1501 ccctgaggag cggctgatca tgaagatcca gcgtgtccgg cggcggacgc tggcgacgga
     1561 cgacctgacg tgacgggccg cgagctgtcg atcgcgttcc agtcggacaa gccgccgggc
     1621 gactacgccg aactggcgcg gctggtcgag ggctacggat tcgatacgct ctcgatctac
     1681 gccgacctgc tcttccagcc gccggtcgtg ccgctggcgg tcgctgccca ggcgaccagc
     1741 cgggtccggc tcgggcctgc ctcgctgaac ccattcacgc tccatccggt cgagatcgcc
     1801 ggccagatcg ccgcgctcga tctgttgtca ggcgggcgag cctacctggg cctctcgcgc
     1861 ggcgcctggc ttgaggacat cggtgtcggg caggagcgcg ccgtctcgcg gatgcgggaa
     1921 gcaatcgacg tgatcgagca cctgctggcc ggccggctgg agccgtacga agggcagcat
     1981 ctccggttga gcgagcatca ccggctgcac tacaccgtcc agcgcgagcg ggtgccactg
     2041 gtcgtcggga gttggggccg gcagacgcta cggctggccg ggcagcgcgc cgatgaggtg
     2101 aaggtcggcg gctcggcgaa tccggatgtt gtcccgatcg tgcgcgactg gatcgctgag
     2161 ggcgcgacga gcgttggccg cgatccagac tcaatcggca tctgcctcgg agcggtgacg
     2221 gtcgttgatg aggatcgcga cgtcgcgcga gcgctcatcc ggcgcgagat ggcgctctac
     2281 ctgccggtcg tcgccggact ggacccgacc gtcagcgttg atcctgacct gctagcgcgt
     2341 atcgaggcgc tcgtcgcccg tggcgaggct gccagcgctg gtgacctgat cccggatgac
     2401 ctgctcgacc gcttcgcctt cgccggcaac ccggatgaca tcatcaggca gtccgaagcg
     2461 ctccttgcgg ctgcggtgac gcgaatcgaa ttcgggacgc cgcatggcgt cactccagcg
     2521 aggggtttga agctgctggg cgagcgggtg ttgccggcgt tgcgctgagg ggtgacctca
     2581 ccccgcccac atctcattca tgaagaagaa cccctcgatc gccaggccga ggccgaaggc
     2641 gatgatgatg gcgaggcgga tggcttcgga gcggatctgg gccagcagga tgtagaccgg
     2701 gaagaggacg agcaggtagc ggttcaggct ctgcgggttg ccggagaggc cgaacagcag
     2761 cgatggcgcg atgacgagcg cgccgtagct ggcgcggacg cgcagccaga ggtagatgcc
     2821 ggccgccagg aagag
//
LOCUS       DASUGJ010000198         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_27525, whole genome shotgun sequence.
ACCESSION   DASUGJ010000198 DASUGJ010000000
VERSION     DASUGJ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27525"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VFV93_04325"
     CDS             <1..1353
                     /locus_tag="VFV93_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5164600.1"
                     /translation="ALAERKPVVVANALDDPSLNNSMSIVGLSLRSIVCVPLILKEEA
                     IGVLYVDSAARSNVFDQTDLDLLVSFASLAATAIENARLHETLLVRSRELENSRARQE
                     SLFRSLSSGLIAIDNAGVITHWNPAAAEMLGARPDNALGARLTDILPAGLATWIGNLA
                     IQAELDSQTFMMSHVWEGPVGTRERAILAGRVARIRDIDGQAEGTVFILNDRTDVVLL
                     EEARRAESAERDRMRELFRRYLAPSVAERLLNSPEAVQLGGSRQDVTILFADVRGFSG
                     FSERHTPEEVVAMLNHYLALATQEIFNELGTLDKFIGDGVMAIFGAPVPVPGPEMAAV
                     RAALAMRASLDRLRNDTGVRVGFGIGLNAGPAVVGNIGTAQLMNYTAIGDVVNVAARL
                     QAEARSGEILISEAILERVRDSVVFEDLSDIYVKGRSVPVTTFKLLGMRESVSATVRQ
                     "
     gene            complement(1370..1807)
                     /locus_tag="VFV93_04330"
     CDS             complement(1370..1807)
                     /locus_tag="VFV93_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164601.1"
                     /translation="MRENELADLLIRMAADRGIEVDAAAEELASFTLPSFVDSRSFRA
                     AVIQSLEQHLRAQVEERLQSIRRRFDSEPQKQASAPVRQPVAPPVSPPPMMAEPAPRF
                     ADPVQVTDATIADIAAQDPPSDNTAADEELPTDATMVWTTRHG"
     gene            complement(1984..>2520)
                     /locus_tag="VFV93_04335"
     CDS             complement(1984..>2520)
                     /locus_tag="VFV93_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5164602.1"
                     /translation="RPRRQQLKYAGRRLAMLYALNGKLRRLRRAGRLGVYSPLDDAVF
                     DEMRDYLEERVWEAGSVIVRQGDPGRDFYVIVEGDVQIELEDPETGREPSVIAQLGPG
                     QYFGELSLLTDQPRNASVVAVSRCRVLSLSRDAFETYLAESPAARQRLNEISKARIRD
                     DESGYGAGSSHEDDIETS"
BASE COUNT          459 a          824 c          742 g          495 t
ORIGIN      
        1 gcgctggccg agcgaaagcc cgtcgtcgtc gccaacgcac tcgatgaccc atcgctgaac
       61 aactcgatga gcatcgtcgg actctcgctt cgctcgatcg tctgcgtgcc gctgattctg
      121 aaggaagagg cgatcggcgt cctctatgtc gattccgccg cgcgttccaa cgtcttcgat
      181 cagacggacc tcgatctgct cgtctcgttc gcatcgctgg ccgcgacggc catcgaaaat
      241 gcccgcctgc acgaaacgct gctggtccgg tcgcgcgagt tggaaaacag ccgcgcgcgc
      301 caggagtcac tcttccgctc gctcagcagt gggctgattg cgatcgacaa cgcaggcgtc
      361 atcacacact ggaatccggc agcggccgag atgctcggcg cgcgacccga caacgcactt
      421 ggtgcgcgcc tgaccgatat cttgccggct ggactcgcga cctggatcgg caatcttgcc
      481 atccaggccg aactggactc gcagacgttc atgatgtcgc atgtctggga aggaccggtc
      541 ggcacacgcg aacgggcgat cctcgccggg cgcgtcgcgc ggatacgcga catcgacggc
      601 caggccgaag ggaccgtctt catcctcaac gatcgcacgg atgtcgtcct gcttgaagag
      661 gccaggcggg cggaaagtgc cgaacgtgat cgcatgcgcg aactcttccg tcgctacctg
      721 gcgccatcgg tcgcggagcg tctgctgaac tcaccagagg cggttcaact cggtggttcc
      781 cgccaggatg tcacgatcct ctttgccgac gtacggggat tttccggctt ctccgaacga
      841 catactcctg aagaagtcgt tgcgatgctg aatcactacc tcgcgctggc aacccaggag
      901 atcttcaacg agctcggaac gcttgacaag ttcatcggcg atggtgtgat ggcgatcttc
      961 ggcgcacccg tgccggtgcc cggccccgag atggctgcgg tccgggccgc actggcgatg
     1021 cgcgcgagtc tcgatcgact gcgcaacgac accggcgtac gtgttggatt cggcattggc
     1081 ctcaacgccg gcccggcggt cgtcggcaac atcgggacgg cgcaactcat gaactacacc
     1141 gctatcggcg atgtcgtcaa cgttgcagcc cgactgcaag cagaagctcg ctccggcgag
     1201 atcctcatca gcgaagcgat tttggagcgc gtgcgcgact ccgtcgtctt cgaggacttg
     1261 tcggacattt acgtcaaggg ccgctcggtg ccggtgacga cattcaagct gctgggaatg
     1321 cgcgagtcgg tcagcgcgac ggtccgacag tgagcgatgt cgctcgacgc tatccgtgcc
     1381 gcgtcgtcca gaccatcgtc gcatccgtcg ggagctcttc gtcggcagca gtattgtcgg
     1441 acggcggatc ctgcgcagcg atgtctgcga ttgtcgcgtc tgttacttgc acgggatccg
     1501 caaaccgtgg cgccggctcg gccatcatcg gcggcggact gaccggcggc gcgacgggct
     1561 ggcgcaccgg ggcgcttgcc tgcttctgag gttcggagtc gaaccggcgg cgaattgatt
     1621 ggagtcgttc ctccacctga gcgcgcagat gctgctccag cgactgaatc actgcggcac
     1681 gaaatgaacg cgaatcgacg aacgatggca gcgtgaatga cgccagctct tccgctgcgg
     1741 catcaacttc gattcctcga tcggcggcca tcctgatgag cagatcggcc agttcgtttt
     1801 ctcgcatctg attgctcctc tgtatcactc acgcccttcc gggcggcccc tcaatatcga
     1861 catgatacgt acatagtaat acgcttttgc aaatcctgcc cgttccgtga ccggatccat
     1921 agatatatcg acaacgaatc ccggttcccg ccgaacggcg acatccgatc tcacgcgaac
     1981 gtctcacgat gtttcaatgt cgtcttcgtg agaactcccg gccccatatc cgctctcgtc
     2041 gtcgcgaatg cgcgccttcg agatctcgtt cagtctctgc ctggcggccg ggctctcggc
     2101 cagatacgtc tcgaatgcat ctctcgacag tgacagtacc cggcagcggc tgactgcgac
     2161 gactgacgca tttcgaggct gatcggtcag cagcgacaac tcaccgaagt attgaccggg
     2221 tccaagctgc gcgatgacgc ttggctcccg tcccgtctcc ggatcttcaa gctcgatttg
     2281 aacgtcgccc tcgacgatca cgtagaagtc acgaccggga tcaccctggc gaacgatcac
     2341 cgatccagcc tcccatacac gctcctccag gtagtcgcgc atctcgtcga acacggcatc
     2401 gtccagcggc gagtacactc ccagccgtcc cgcacgacgc agcctgcgca acttgccgtt
     2461 tagcgcatac agcatcgcca ggcgtcttcc agcgtacttg agttgctgcc gccgcgggcg
//
LOCUS       DASUGJ010000199         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1694988, whole genome shotgun sequence.
ACCESSION   DASUGJ010000199 DASUGJ010000000
VERSION     DASUGJ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694988"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFV93_04340"
     CDS             complement(<1..773)
                     /locus_tag="VFV93_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5164603.1"
                     /translation="MHVTLFVLTLLFTTAVGALHYLSFISEFGRRPAALTWGSLAEGL
                     WYSLTLLAILGAHEFGHYYFCRRYDVDASLPYFIPFPITLTGTLGAVIKIREPFPNRT
                     VLFDIGVGGPIAGFVVLVPALFIGMAMSNVVPMPTGQGVFYMGEPLLFKLAAWLNFGT
                     IPANQTINIHPIVFAAWFGMLATALNLLPFGQLDGGHITYATLDRWSTQISLATVATA
                     IAMTYNSTSWLFMTAMMVLMLLLLGPRHPRVIYEHEPLAR"
     gene            complement(882..1190)
                     /locus_tag="VFV93_04345"
     CDS             complement(882..1190)
                     /locus_tag="VFV93_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5164604.1"
                     /translation="MIVITPEESLSQMIKVDIVNEVSKIADITKVKAEVAVDAVFDAM
                     RLSMQRGERIELRGFGVFQVKPRKRGIGRNPRTGKEVRIPPGRTIRFKPGKDLQNIGG
                     "
     gene            1264..>2029
                     /gene="der"
                     /locus_tag="VFV93_04350"
     CDS             1264..>2029
                     /gene="der"
                     /locus_tag="VFV93_04350"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX5164605.1"
                     /translation="MPTVVLVGRPNVGKSTLFNRVTRTRRSIVTSIAGTTRDVIAEPA
                     GWLDTPFRLVDTGGMFGASTDPLHELVIAQGRKAVETADVIVFVVDGREGLVPADEEI
                     AASLRKSKAPVLLAVNKTDDKRARGRVLEFHELGFDPVIEIAAEHGEGVGDLLDEVIR
                     RLPARTEREDDTSEETAIAIVGRPNVGKSSLLNRLLKEERSIVSEMPGTTRDTVDAML
                     KWQKRTFRIVDTAGIRRPGRVARSGQLESLSVILARR"
BASE COUNT          411 a          628 c          649 g          341 t
ORIGIN      
        1 cgtgcgagcg gctcgtgctc gtagatgacg cgggggtgcc gtggccccag cagcaacagc
       61 atcaagacca tcatcgccgt catgaacagc cagctggtcg agttgtaggt catcgcgatc
      121 gccgtggcca ccgtcgccag cgaaatctgt gtcgaccagc gatcgagcgt cgcgtaggtg
      181 atatggccgc catccagctg gccgaagggc agaaggttga gcgccgtcgc cagcatcccg
      241 aaccatgccg caaagacgat cggatggatg ttgatcgtct ggttggccgg aatcgtaccg
      301 aagttgagcc aggccgccag cttgaagagc agcggctccc ccatatagaa gacgccctga
      361 ccggtcggca tggggaccac gttcgacatc gccatcccga tgaacagcgc cggcacgagc
      421 acgacgaatc ccgcgatggg cccgccgacg ccgatgtcga acaggacggt acgattggga
      481 aacggctccc tgatcttgat caccgcgccg agcgtgccgg tcagtgtgat gggaaacggg
      541 atgaaatacg gaaggctggc gtcgacgtcg taacggcggc agaaatagta atggccgaac
      601 tcgtgcgcgc caaggatcgc cagcagcgtc aggctgtacc acagcccctc ggccagcgag
      661 ccccaggtga gcgctgccgg gcggcggccg aactccgaga taaacgacag ataatggagc
      721 gctcccacag cggtcgtgaa caggagcgtg aggacgaaca gcgtgacgtg caaccagacc
      781 cggtcgcgga acttgcgggg aggacgccga acgtaatacg tgtcgatcgg aagtggagac
      841 gccgtgaacg ggcgccccgc ctctctgggc ggatacgcca atcagcctcc gatgttctgg
      901 agatccttgc cgggcttgaa tcggatcgtt cgaccgggcg ggatccggac ttccttcccc
      961 gttcggggat tgcgcccgat gccgcgcttg cgtggtttca cctggaacac gccgaaacca
     1021 cgaagctcaa tccgttcgcc tcgctgcatc gagagccgca tcgcgtcgaa caccgcgtca
     1081 acagccacct cggctttgac cttggtaatg tccgcgatct tcgacacctc gttgacgatg
     1141 tcaaccttga tcatttggct tagcgactcc tcaggagtga taactatcat aaatagttgc
     1201 acttacgctg tcaagcatcg tgctaactag actttgaaaa tgccagtcag acgtgccctt
     1261 cagttgccca cggtcgtgct ggtcggacgg ccgaacgtcg gcaagtcgac gttgttcaat
     1321 cgcgtcacgc ggacgcgccg ttcgatcgtc acatcgatcg cggggacgac ccgcgacgtc
     1381 atcgccgagc ccgccggctg gctcgacaca ccattccgcc tcgtggatac cggtggaatg
     1441 ttcggagcga gcaccgatcc gcttcacgag ctcgtgatcg ctcaggggcg caaagccgtc
     1501 gaaacggcgg acgtcatcgt ctttgtcgtg gacggccgcg agggcctcgt cccggcagac
     1561 gaggagattg ccgcctcttt gcggaaatcg aaggctccgg tcctcctggc cgtgaacaaa
     1621 accgacgata aacgggcccg cggccgcgtc ctcgaattcc acgagctcgg attcgacccc
     1681 gtgatcgaga tcgccgccga gcacggcgaa ggagtcggcg acctgctcga cgaagtcatc
     1741 cgtcgattgc cggcgaggac cgagcgggaa gatgacacct ccgaagagac cgcgatcgcc
     1801 atcgtcgggc ggccgaatgt cggcaagtcg tcgctcctca accggctgct caaagaggag
     1861 cggtcgatcg tgagcgagat gccgggaacg acgcgcgata ccgtcgacgc gatgttgaaa
     1921 tggcagaagc ggacgttccg tatcgtggat accgcgggga tccggcgtcc ggggcgcgtc
     1981 gcgcgaagcg ggcagctcga gtcgctgagc gtcatcctcg cccgtcgcg
//
LOCUS       DASUGJ010000200         4186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_305706, whole genome shotgun sequence.
ACCESSION   DASUGJ010000200 DASUGJ010000000
VERSION     DASUGJ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305706"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFV93_04355"
     CDS             <1..395
                     /locus_tag="VFV93_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX5164606.1"
                     /translation="GYLTYVHYNEDALVCTTGGCETVQQSEYSTIGGIPIAIFGLLMF
                     TTVTLLAAARLTGRLPISFETATMLAWTMLLTSLLYYAYLTYLEIFELEAICQWCVSS
                     SIVALLMFVLESILLFRDAPDEQVIGDR"
     gene            complement(502..1707)
                     /locus_tag="VFV93_04360"
     CDS             complement(502..1707)
                     /locus_tag="VFV93_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5164607.1"
                     /translation="MTATTAPGIEELFSDRAKLARPGMVGTNPRAIADVIAFSGGYPD
                     PGSLPIQDIIESTRMALERDGEWALQYAFGSGVPELVEQLLAKLARDQGITAGVENIL
                     ITSGSSQALALIYELFVNPGDVMLAEAPWFMGSIWRARASGADIREIPLDDQGLDVVE
                     LERVLNGLKAEGKRAKFLYLVPTFQNPTGITYTLERRKRIVELAQEHGLPILEDDAYF
                     DLRFEGETLPPLYTLDDQGLVMYCGTFSKIMSAGMRLGWCVASAPILARLAGLKTDSG
                     TNPFASHVAAQFAASGTLTEHIAHLRPLYKHRRDVMLAALEEQMPEGTTWTVPEGGFF
                     IWMTLPEGVTCTEIAPIAQQRGVGIGMGTMFYANGGGENKVRLSYSFNDDDQIREGIG
                     ILADVIRGE"
     gene            1873..2445
                     /locus_tag="VFV93_04365"
     CDS             1873..2445
                     /locus_tag="VFV93_04365"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5164608.1"
                     /translation="MTATASPLTPTITRRLFTVHEFHEMAKAGILGEKERVELIEGEI
                     VQMSPIGSSHVLGISRLVHLLVPLVGDEGLISPQGSFRISEHTELLPDFVIMRNKEYD
                     EIPGPADALLVIEVSDSLPSRDLTSKAAIYAGAGVQEYWVWDVNRHVVHQFAEPSDGQ
                     FTYRRLVKLGETIASVRLQAVSFTVVDISR"
     gene            2453..3049
                     /locus_tag="VFV93_04370"
     CDS             2453..3049
                     /locus_tag="VFV93_04370"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5164609.1"
                     /translation="MRPSMKRGVTMVAVPTRHKFTVDELKQFVAAGVLSEDDRVELIE
                     GDLIDMSPINEPHEDAIDMFNDHLVDQIGRTARVRFGSPVRLSDLSIVQPDMSVVRRQ
                     RYLSSHPRPDDVLLLIEVSDTTLSFDLRTKVPLYARSGVPEVWVVDVNASVIHRFLRP
                     EGSIYQITEQLRAGDTLTIEALPNVSLAVADIFDPSRD"
     gene            3129..>4186
                     /locus_tag="VFV93_04375"
     CDS             3129..>4186
                     /locus_tag="VFV93_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5164610.1"
                     /translation="MSSDELVYRTATDLARSIRQKELSAREVMQAHLAQIARVNPKVN
                     AIVTRISDDEALALADAADEQLARGDEVGVLHGLPIAHKDLEDTAGMRTTYGSPIFAD
                     NVPKHDTLMISRLKAAGALTIGKTNVPEWGAGSQTFNPVFGPTLNPYDTTRTCGGSSG
                     GAAVTLACGMAPIADGSDMGGSLRNPGNFNNVVGLRPSPGRVPVSGSSMPWIPLGVKG
                     PMARTVADVALLLSTIAGYDRHVQLAIEESGDMFRQPLDRDFSGVRIAFSADLGGLPV
                     DPRVRAVLEAGRPVFESLGCSVEEDAPDFSGADDAFRDLRAFSFSTQHVDLIARHREM
                     YKDTVIWNVEEGLKLSGV"
BASE COUNT          766 a         1306 c         1308 g          806 t
ORIGIN      
        1 ccggctacct gacctacgtc cactacaacg aggacgcgct ggtctgcacg accggcggct
       61 gcgagacagt ccagcagagc gaatacagca cgattggcgg catcccgatc gccatcttcg
      121 gcctgctgat gttcacgacc gtgacactcc tggcggcggc ccggctgacg ggccgcctgc
      181 caatctcgtt cgagacggcg acgatgctgg cctggacgat gctactgacc agcctgctct
      241 actacgccta cctcacctac ctcgagatct tcgagcttga ggcgatctgc cagtggtgtg
      301 tgtcctcgtc gatcgtcgcg ctgctgatgt tcgtgctgga gtcgatcttg ctgtttcggg
      361 atgcgccaga cgagcaggtg ataggtgata ggtgataggt gataggtgtt agggatcggt
      421 tctcaggaat cagcatgtcg cattcctccc gcaccgacca aaccctaaca cccatcacct
      481 ggcacctatc acctaacgca cctactcccc gcgaatgacg tcagccagaa tgccgatgcc
      541 ttcgcggatc tggtcgtcgt cgttgaagga gtaggacagg cggaccttgt tctcgccgcc
      601 gccgttggcg tagaacatcg tccccatccc gattccgacg ccgcgttgct gggcgattgg
      661 cgcgatctcg gtgcaggtga ccccctccgg cagcgtcatc cagatgaaga agccgccctc
      721 gggaacggtc caggtcgtgc cctccggcat ctgctcttcg agcgcggcca gcatgacatc
      781 gcggcgatgc ttgtagagtg ggcgcaggtg ggcgatgtgc tcggtcagcg ttccggatgc
      841 cgcgaactgg gctgcgacgt ggctggcaaa cgggttcgtg ccggaatcgg tcttcaagcc
      901 ggccaggcgc gcgagaatcg gcgcactcgc cacgcaccag ccgagccgca tcccggccga
      961 catgatcttc gagaacgtcc cgcagtacat gaccagcccc tggtcatcca gcgtgtagag
     1021 tggcggtagc gtctcgccct cgaagcgcag gtcgaagtag gcgtcgtcct ccaggatcgg
     1081 cagcccgtgc tcctgggcca gctcgacgat ccgcttgcgg cgctccagcg tgtaggtgat
     1141 gccggtcgga ttctggaagg tcgggacgag atagaggaac ttggcccgct tgccctcggc
     1201 cttgagcccg ttcaggacgc gttccagctc gacgacatcc agcccctgat cgtcgagcgg
     1261 gatctcgcgg atatcggcgc cgctggcccg cgcccgccag atcgagccca tgaaccaggg
     1321 cgcttcggcc agcatgacat cgccgggatt gacgaacagc tcgtagatca acgccagcgc
     1381 ctgtgacgag ccgctggtga tgaggatgtt ctcgacgccg gccgtgatcc cctgatcgcg
     1441 ggccagcttg gcgagcaact gttcgaccaa ttccggcacg ccggagccga aggcgtattg
     1501 caacgcccac tcgccgtcgc gctccagcgc catccgcgtt gactcgatga tgtcctgaat
     1561 cggtagcgac cccggatcag gatagccgcc ggagaaagcg atcacatcgg caatcgcccg
     1621 tgggtttgtg ccgaccatcc cgggccgagc cagcttcgcg cggtccgaaa acaactcctc
     1681 gatgcctggc gctgtcgtcg ccgtcatgct cactcccctg ttcaccccgg ctgccgcacg
     1741 accgacaatc gcgcccgcca gtggctctgc tgcttcgcct cagaaaacgt tggcgtatta
     1801 cagcacggac gcgcgaagac gccaaccccg tacgctatgc ttgcagcacg gtcttccgtc
     1861 gaaggaggca ccatgactgc gactgcttcg ccactaacgc caacgatcac acgacgcctg
     1921 ttcaccgtcc atgagttcca cgagatggcg aaggcaggca ttctcggtga aaaagagcgc
     1981 gtcgagctga tcgaaggcga aatcgttcag atgagtccga tcgggagttc tcacgtgctg
     2041 ggcatttcgc gcctggtgca tctgctcgta ccgctcgtcg gtgatgaagg gctgatcagc
     2101 ccacagggtt cattccgtat cagcgagcac accgagttgc ttcccgattt cgtcatcatg
     2161 cgaaacaagg aatacgacga aattccggga cccgccgacg cgctgctcgt cattgaagta
     2221 tcggacagct tgccttctcg cgatctcaca tcgaaggcgg ccatctacgc cggagctggt
     2281 gtgcaggagt attgggtctg ggatgtgaat cgacatgttg tccaccagtt tgcggagccg
     2341 tcggacggcc agttcaccta tcgaagacta gtgaaactag gtgagacgat cgcgtcggtg
     2401 aggttgcagg cggtgtcgtt cacggtcgtc gacatttcgc gctagatatt cggtgcggcc
     2461 atcgatgaaa cgaggagtca ctatggttgc tgttccgaca cgacacaagt tcactgtcga
     2521 tgaactcaag caattcgtcg ccgccggtgt cctttctgag gatgatcggg ttgaactgat
     2581 cgagggagac ctgatcgaca tgagcccgat taacgagccg cacgaagatg caatcgacat
     2641 gttcaacgat catctcgttg atcaaatcgg aagaactgca cgtgtcagat tcggaagtcc
     2701 cgttcgtctg agtgatttgt cgatcgttca gcctgacatg tcggttgtac gccggcaacg
     2761 gtatctctcc agccacccgc gtccggacga cgtgctgctc ctgattgaag tctcggatac
     2821 cacgctgtca ttcgacctcc gaacgaaggt gccactctac gctcgctcgg gtgttccaga
     2881 ggtatgggtt gttgacgtca acgcgagcgt gatccatcga ttcttgcgac ctgagggatc
     2941 gatctaccag attactgagc agcttcgtgc gggcgatacg ctgacgatcg aggcgctacc
     3001 gaacgtctcg ctcgcagtcg ccgatatctt cgacccatcg cgcgattgac cgtttccgtg
     3061 gacaataccc ccacgctgat tgctggcggc tgccggccgt cggtttcgga ctgaggggag
     3121 tgggaacggt gtcgagcgac gaactggtct acaggacggc gacggatctg gcgcgcagta
     3181 tccgtcagaa agagctctcg gcgcgcgagg tgatgcaggc gcatctggcg cagatcgcgc
     3241 gggtgaatcc gaaggtcaac gcgatcgtaa cgcgtatcag cgacgacgag gcgctggcgc
     3301 tggccgacgc cgccgacgag cagctggccc gtggcgacga ggtcggtgtg ctgcacggcc
     3361 tgccaatcgc tcacaaggac ctcgaagaca cggccgggat gcggacgacc tacggctcgc
     3421 cgatctttgc tgacaacgtg cccaagcacg acacgttgat gatctcgcgc ctcaaggcgg
     3481 ccggcgcgct gacgatcgga aagacgaacg tgccggagtg gggcgcgggc tcgcagacgt
     3541 tcaacccggt cttcgggccg acgctcaatc catatgacac aaccaggacc tgcggcgggt
     3601 cgagcggcgg cgcagcggtg acgctggcct gcgggatggc gccgatcgcc gatggcagtg
     3661 acatgggcgg ctcgctgcgc aatcccggca acttcaacaa cgtcgtcggc ctgaggccgt
     3721 cgccgggccg tgtgccggtc tcgggcagct cgatgccgtg gatcccgctc ggcgtcaagg
     3781 gcccgatggc gcggaccgtg gccgacgtgg cgctgctgct ctcgacgatt gccggttacg
     3841 accggcacgt ccagttggcg atcgaggagt ccggcgacat gtttcgccag ccgctcgatc
     3901 gtgacttctc aggcgtccgc atcgccttca gtgccgatct tggcgggttg ccggtcgatc
     3961 cgcgcgtccg tgccgtcctg gaggctggcc gccccgtctt cgagagtctc ggttgctcgg
     4021 tcgaggagga cgcgcccgat ttcagcggcg ccgacgacgc cttccgcgac ctgcgcgctt
     4081 tcagtttctc gacccagcac gttgacctga tagctcgcca ccgcgagatg tacaaggaca
     4141 ccgtcatctg gaacgttgag gagggcttga agctgtcagg cgtcga
//
LOCUS       DASUGJ010000201         6802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1973500, whole genome shotgun sequence.
ACCESSION   DASUGJ010000201 DASUGJ010000000
VERSION     DASUGJ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973500"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /locus_tag="VFV93_04380"
     CDS             complement(<1..1720)
                     /locus_tag="VFV93_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5164611.1"
                     /translation="MAARRWRFVLGGVGLGLVLVQFLSLAVGWRPSTPVAVAAPPADG
                     PIQATWRRTDGPVLSGAAGRTWMWGPAAITDLLSEPYAESPGGMRAVQYFDKSRMEIT
                     LPGADQSSPWYVTNGLLVVELVSGRMQLGHASWEQFAPADVNVAGDPNGRSGPTYAAL
                     AGARELPPLADGALIIQQIDHAGTITTDTALASYGVQAAYRVTVEGIDHQIAAPFWSF
                     MQSEGVVEIDGVLVNAPLFSNPFYATGLPIIEPYWTTVPVGGQPKDVLIQCFERRCLT
                     YTPTNDAGWQVEAGNVGQHYLRWRQQHAPGQVTATATAQTIATATATATSTEASQSAT
                     ATATATGTTPAACDPAYPDFCIPPPPPDLDCDDIGQGGFTVLPPDPHHFDNDGNGIAC
                     EGNPATATKPGGGDPDPTATATATRTNTPTRTPTPTKTPTPTKTATPTKTPTPTQTAT
                     ATFTPTATMTPTPDPNGDAATCLSATEEEFVELLNAYRADLGLQPVVASAKLTVAAYR
                     HSLDMGTRNYLAHDTKEPLPDGQSGPTYQDRIRDAGYTGWTIATESIAGGNQVYASAE
                     ALFDIWL"
     gene            complement(1900..4479)
                     /locus_tag="VFV93_04385"
     CDS             complement(1900..4479)
                     /locus_tag="VFV93_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5164612.1"
                     /translation="MNDWLGGTLGNYSIEESLGTGGMGRVYRAVHIHINRVAAVKVLH
                     EQFQVDPAFQARFLREAQAIAALQHPNIVDVYDFNDDQGVFYLVMEYVPDGSLRSMLK
                     EHAERGTALPLAQGLELIRQAAEALDYAHRSGMVHRDIKPDNLLLRHDPITDTFIVKV
                     ADFGLARLGDSVTLTAEGQAMGTPAYMSPEQCQGTDLDGRSDIYSLGVVLYEVITGAT
                     PFVMRSLTDAIYKHVYVEPPSPLDVRPDLPDELAAIALRCMAKNPVDRFQTAGELAAA
                     LARLTRTDLRDVEQAVTLAEPLPPVDQLRVALDSGEAPLTPGEATALTVTLVNAGRTA
                     VHASISIDGIPEDWISTPPRDVLLAPGAEISGPIRVAVPATATGPGEYPVVVRAAALE
                     NPALRAEATTRLLLRPVQPVGAGAGAGAGSGGGGTLPPGGEAIEDRGRPGWLAGAAVV
                     LLLLVVGAIAYALLRPDGGDDEDTGVVADGTPTAGVATTTATIASTASATDTAAGATA
                     TAVVIVGPGETPTVKPAATATLAAATETPAPAVLPETIVFTAGRNLADSTGTLDIYQM
                     NPDGSDQRPLISQPNDDWLAAISPDGRRVVWVSRQHGNHQLYIAMVDGTGVRRLTTST
                     GDDLYPAWAPDSVHILFSRNEDGDDELYVLNVDNGTLVRLTDNDAYDGFAAWSPDGTR
                     IAGTSSLAGNNDIYVSEGVLRDVSADRLTDDPANDFNPVWSPDGTQLAFVSNRGGDND
                     IYRMQADGSGLIPLTSSPADEFAPAWSPDGSRIAFCRQTGNACDVVVINTDGSGELVL
                     ASNASQHDPDITWSPDSMKLAYLSEESGNYDVFTVALFGSPPARLTDAPGNDANLTWF
                     APK"
     gene            complement(4747..5187)
                     /locus_tag="VFV93_04390"
     CDS             complement(4747..5187)
                     /locus_tag="VFV93_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164613.1"
                     /translation="MQQIVYAMQFHGEGAPADDGTMHAATRAPSMRITSSVGGAGLTG
                     SIDEVDGGEATFTSVVRMTGETSFIETGTITFGDGNSVDFSTVGEGFMGPSPQPGLLH
                     GVISWQIDSGTGQFAGASGLITSNFTFSEQGEVIDNQFGVIWLA"
     gene            5363..5890
                     /locus_tag="VFV93_04395"
     CDS             5363..5890
                     /locus_tag="VFV93_04395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5164614.1"
                     /translation="MADVVRVLIETELGEIEAKIEVERAPITAANFLRYVDAGRYDGG
                     RWHRTVLPDNQPNDAIKIEVIQGGTKPKRWRWNERPIPLERTSVTGLAHHDGTLSMSR
                     FAPDSAVSDIFVCIGDQPDLDFGGLRNPDGQGFAAFGQVTRGMDVVRAIQHAPHDGQR
                     LTPPITIVRIRRIGA"
     gene            5947..6429
                     /locus_tag="VFV93_04400"
     CDS             5947..6429
                     /locus_tag="VFV93_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5164615.1"
                     /translation="MTQIRAARASDYDLVLPLFLGLREFSRAGHPPQADDFGSVLAAS
                     REYLREVLARGPECTTLLALAEDGRLAGYVVVSIHEPNPLTSSGAVRSGSIDELFLDT
                     TSRGQGVGRLLVSAACDWLAELRAERVEVGAYAWNAAAIAFYKREGFAPQTITFTKDI
                     "
     gene            6492..>6802
                     /locus_tag="VFV93_04405"
     CDS             6492..>6802
                     /locus_tag="VFV93_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX5164616.1"
                     /translation="MTTSDAEPETTPGSQAGSLREVAGLMFRLGWTAFGGPAAHIAML
                     RDEVINRRKWMDEQYFLDLLGATNLIPGPNSTELVIHSSYQRAGWRGLIVGGSLFILP
                     AV"
BASE COUNT         1114 a         2274 c         2234 g         1180 t
ORIGIN      
        1 cgagccagat gtcgaacagc gcctcggcgc tggcgtagac ctggttcccg ccagcgatgc
       61 tttccgtcgc gatcgtccag ccagtatagc cggcgtcacg aattcggtcc tgataggtcg
      121 gcccggactg gccgtcaggt aacggctcct tcgtatcatg cgcgaggtaa ttacgcgtgc
      181 ccatgtcgag gctgtgccgg taggccgcga ctgtcagctt cgctgaggcg acgaccggtt
      241 gcagtcccag atcagcccgg taggcattca ggagttcaac gaactcctct tcggtcgcgc
      301 tcaggcaggt cgcggcgtcc ccgttcgggt cgggcgtcgg cgtcatcgtc gccgttggcg
      361 tgaaggtggc cgtcgccgtc tgggttggcg ttggtgtctt cgtcggcgtc gcggttttcg
      421 tgggtgtcgg tgtcttcgtt ggcgtcggag ttcgcgttgg cgtgttcgtg cgcgtcgcag
      481 tcgcggtcgc ggtcggatct gggtcgccgc caccgggctt cgtcgccgtc gccgggttcc
      541 cttcgcaggc gatcccgttg ccgtcgttgt cgaaatggtg cgggtcgggc ggcaggaccg
      601 tgaacccgcc ctgcccgata tcgtcgcagt ccagatccgg cggtggcggc gggatacaga
      661 agtcaggata ggcagggtcg caagccgccg gcgtcgtgcc ggtcgccgtc gcagtcgccg
      721 tggctgactg gctcgcctcg gtgctggtgg cggtcgcggt cgcggtcgcg atcgtttggg
      781 ccgtcgcggt agccgtcacc tgtcccggcg cgtgctgctg ccgccagcgc agatagtgct
      841 ggccgacgtt gccggcctcg acctgccagc cagcgtcgtt ggtcggcgtg taggtcaggc
      901 aacgccgctc gaagcactgg atcaggacat ccttcggctg gcctccgacc ggtaccgtcg
      961 tccagtacgg ctcgatgatc ggcagtcccg ttgcgtagaa cgggttgctg aacagcggcg
     1021 cgtttaccag cacgccatcg atctcgacga cgccttcgga ctgcatgaac gaccagaacg
     1081 gcgccgcgat ctgatggtcg atcccctcga cggtgacgcg ataggcggcc tgaaccccgt
     1141 agctggccag cgccgtgtcg gtcgtgatcg tcccggcgtg gtcgatctgc tggatgatca
     1201 gcgcgccgtc cgccagtggc ggcagttcgc gagcgcctgc cagcgccgcg taggtcgggc
     1261 cgctccggcc gttcggatca ccggcgacgt tgacatcggc cggtgcgaac tgctcccagc
     1321 tggcatgccc gagctgcatc cggccgctga ccagctcgac gaccagcagg ccgttggtga
     1381 cgtaccacgg cgacgactgg tctgcgcccg gcagcgtgat ctccatccgc gacttgtcga
     1441 aatactggac agcgcgcatc ccgccgggcg attccgcata tggctcgctc agaagatcgg
     1501 tgatcgccgc cggaccccac atccaggtcc gcccggccgc tccggagaga accgggccat
     1561 ccgtccgtcg ccaggttgcc tggatcggcc catcagccgg tggcgcggca acggcgaccg
     1621 gcgtcgatgg tcgccagcca actgccagcg acagaaattg gacgagcacc aaaccgaggc
     1681 caacaccacc cagcacgaaa cgccagcgtc gcgccgccac aatccccccg cagatacaca
     1741 ccacacgatt ctcaaacggg agtattgtct gcgggaccgt cacaaattgc aagcgggaga
     1801 cgcaactcgg atcggcgttc caatcaatcc atcaatcgca atctcccaat ccggtacggc
     1861 cgatattgcg attgatggat tgcgaccgcg ccgaaacacc tacttcggcg cgaaccaggt
     1921 caggttcgcg tcgttgcctg gagcgtccgt caaccgggca ggcggggagc cgaagagggc
     1981 gacggtgaag acatcgtagt tgccggactc ctcgctgagg tacgccagct tcatgctgtc
     2041 cggcgaccag gtgatatccg gatcgtgctg gctggcgttg ctggccagca ccagttcacc
     2101 gctcccatcg gtgttgataa cgaccacatc gcaggcgtta ccggtctggc ggcagaacgc
     2161 gatccggctc ccgtccggcg accaggccgg agcgaactcg tcggcaggtg aggatgtcag
     2221 cggtatcagg cccgatccgt cggcctgcat ccggtagatg tcgttgtcgc caccgcgatt
     2281 cgatacgaac gcgagctgtg ttccatccgg cgaccagacc gggttgaagt cgttggccgg
     2341 gtcatccgtc aggcggtcgg cgctcacatc gcgcaggacg ccctcggaga cgtagatgtc
     2401 gttgtttccg gccaggctgc tcgtcccggc gatccgggtg ccgtccggtg accacgccgc
     2461 gaagccgtca taggcatcgt tgtcggtcag ccggacgagt gtgccgttgt cgacgttgag
     2521 tacatacagc tcatcatcgc cgtcctcatt gcgggagaag aggatatgca cgctatccgg
     2581 cgcccaggcg gggtagaggt catcgccggt cgaggtcgtc agccgccgaa cacccgtccc
     2641 gtcgaccatc gcgatataga gttggtggtt gccgtgctgg cgcgagaccc agacgacccg
     2701 ccgcccatcc ggcgagatgg ctgccagcca gtcgtcgttc ggctggctga tcaacggccg
     2761 ctggtccgat ccgtccggat tcatctggta gatgtcgagt gtcccggtag aatccgcgag
     2821 attccggccg gccgtgaaga cgatcgtctc cggcaggacg gctggtgccg gcgtttcggt
     2881 cgcggcggca agcgtcgcgg tcgcggccgg cttcacggtc ggcgtctcac ccggcccgac
     2941 gatgacgaca gccgtcgccg tcgcaccagc ggcagtgtca gttgcgctgg cggtgctggc
     3001 gatcgtggcg gttgtcgtcg ccacaccggc cgttggcgtt ccatcagcaa cgacgccggt
     3061 gtcttcatcg tctccaccat ccggacgcag cagcgcgtag gcgatcgcgc cgacgaccag
     3121 caggagcagc acgacggccg cgcccgccag ccagccgggc cggccgcgat cctcaatcgc
     3181 ctcgccgccg ggtgggagcg tgccgccgcc acccgaacca gcgccagcac cggcacctgc
     3241 gccaaccggc tggaccggtc gcagcagcag tcgcgtcgtc gcctcggctc gcagggcggg
     3301 gttctcgagc gctgcggccc gaacgacgac cggatactcg ccagggccgg tcgccgtcgc
     3361 cggcaccgcg acgcgaatag gcccgctgat ctccgcgccg ggagccagca gcacatcgcg
     3421 cggcggcgtc gagatccagt cctccgggat gccgtcgatg ctgatgctgg cgtgcaccgc
     3481 cgtccggccg gcgttgacga gcgtgacggt cagtgccgtc gcctcacccg gcgtcaacgg
     3541 cgcctcgccg ctgtccagcg cgacccgcaa ctgatcgacc ggcggcagcg gctcggccag
     3601 cgtgacggcc tgctcgacat cgcgcaggtc cgtccgtgtc agtcgcgcca gcgccgccgc
     3661 aagctcacct gccgtctgga agcggtcaac cgggttcttc gccatgcagc gcagggcgat
     3721 cgcggccagc tcatccggca gatcggggcg cacgtcgagc ggcgacggcg gctcgacgta
     3781 gacatgcttg tagatcgcgt cggtcagcga gcgcatcacg aacggcgtcg cgccggtgat
     3841 cacctcatag agaacgacac ccagcgagta gatgtcactg cgcccgtcga ggtcggtccc
     3901 ctggcactgc tccggcgaca tgtaggcagg cgtgcccatc gcctgcccct cggccgtcag
     3961 cgtcacgctg tcgcccagcc gcgccaaccc gaagtcggcg accttaacga tgaaggtatc
     4021 ggtgatcgga tcgtgccgga gcagcaggtt gtccggcttg atgtcgcggt ggaccatccc
     4081 gctgcggtgg gcgtaatcga gtgcctcggc tgcctggcgg atcagttcca gcccttgcgc
     4141 aagcggcagc gccgtacccc gctccgcatg ctccttgagc atcgagcgca gactgccgtc
     4201 cggcacatac tccatgacca gatagaagac gccctgatcg tcgttgaagt catagacatc
     4261 gacgatgttg ggatgctgaa gggcagcgat cgcctgcgcc tcgcggagga aacgcgcctg
     4321 aaaggctgga tcgacctgga actgttcgtg gagcaccttg acggccgcga cccggttgat
     4381 atggatatgg accgcccggt agaccctccc catcccgccg gtgccgagcg attcctcgat
     4441 gctgtaattc ccgagcgtcc cgcccagcca gtcgttcatc ctgccctctg caagcagcgt
     4501 cgcgcgttat cgcacacccc tcgcggggaa tgtctgcatg ctgtcaggat agcccgagcc
     4561 gtcaagcgcc acaccccgat atgtcatcct gagtcctaag ccgaaggatc tctcctgagg
     4621 atgacaaacg ggaggccgtc acattcaccg ccgtggaccg acgagtgcct cgacaggcgg
     4681 agccagcaac accgtcgaat gcaccgattg agttgatgtt ctcgttcaaa gtccagcgtg
     4741 gagcgctcag gcgagccaga tgacaccaaa ctgattgtcg atcacctcgc cctgctccga
     4801 gaacgtgaag ttggaggtaa tcagcccgct ggcgccggcg aactggccgg ttccggagtc
     4861 gatctgccat gagatcacgc cgtgcagcag ccccggctgc gggcttggtc ccatgaaccc
     4921 ctcgccgacc gtgctgaaat cgacgctgtt gccatcgccg aacgtgatcg tgccggtctc
     4981 tatgaagcta gtctcgccgg tcatccggac gaccgaagtg aaggtcgcct cgccgccatc
     5041 aacctcgtca atcgatccag tcaggccggc cccaccgacc gaggaggtga tgcgcatgct
     5101 cggcgcacgc gttgcggcgt gcatcgtgcc gtcgtcggcc ggagcgcctt cgccgtggaa
     5161 ctgcatcgca taaacgatct gctgcatcgc gttcctgctt tcctgagttg tctgctggct
     5221 cctgccgcaa tctagcactg ccacgccccg ttgtcacgcc ccggttcagc ggatcccgga
     5281 cattgagcgt tccggcatgc tggcgtatcc tgtgcgtcct gcgccgaggc gcgttcgcat
     5341 taaccgggag ggggcagccg gcgtggcaga cgtggtccgt gtcctgatcg agacggagct
     5401 cggcgagatc gaggcgaaga tcgaagtcga gcgggcgccc atcaccgccg cgaactttct
     5461 gcgctacgtc gacgccggac gctacgacgg cggtcgctgg catcggacgg tcctgccgga
     5521 caaccagccg aacgatgcga tcaaaatcga ggtcatccag ggcggcacga agccgaagcg
     5581 ctggcgctgg aacgagcgac cgatcccgct cgaacgaacg tccgtcaccg gtctcgccca
     5641 ccacgacggg acactctcga tgtcccgctt cgcgcccgac agcgccgtct ccgacatctt
     5701 cgtctgcatc ggcgaccagc ccgacctcga cttcggcggg ctgcgtaacc ccgacgggca
     5761 ggggttcgcc gccttcggcc aggtaacgcg tgggatggac gtcgtccgcg ccatccagca
     5821 cgcaccccac gacggacaac gcctgacgcc gccgatcacg atcgttcgca ttcgacgtat
     5881 cggcgcctga atgagtgtat gtcgcgcgat acccgatata aagaccagag cgggagctac
     5941 ggcaacatga cgcagatacg ggctgcgcgt gccagtgact atgatctcgt tctgccgttg
     6001 tttctggggc tacgcgaatt cagccgcgca gggcatccgc cgcaagcaga cgacttcggg
     6061 tcggttctgg cggcaagccg cgagtacctg cgtgaggtgc tggcgcgagg cccggaatgc
     6121 acgacgctgc tggcgctggc cgaggatggg cgcctggcag gctacgtcgt cgtcagcatt
     6181 cacgagccga acccgctcac cagttccggc gcggttcgct cgggatcgat cgacgagctg
     6241 tttctggata caacctctcg cggccagggc gtcggccgct tgctggtcag cgccgcctgc
     6301 gactggctgg ccgagctgcg ggccgaacgt gtcgaggttg gagcctacgc ctggaacgca
     6361 gccgcgatcg ccttctacaa gcgtgaaggg ttcgcgccgc agacgatcac attcacgaaa
     6421 gatatttgac agcgctgcct tgatcgacgc aaggtctccg gccagggagc gcgggctacg
     6481 agggaacgcg agtgacgaca tcggacgctg agccagagac aacgccaggc agtcaggccg
     6541 ggtcgctgcg cgaagtcgcc gggctgatgt tccggctggg ctggacggcg ttcggtggac
     6601 cggccgccca catcgcgatg ctacgcgacg aggtgatcaa tcggcgcaaa tggatggacg
     6661 agcagtattt cctcgacctg ctcggcgcca ccaacctgat tcccggccca aactcgaccg
     6721 aactggtcat tcattcgtcg taccagcggg ccggctggcg ggggctgatc gttggcggct
     6781 cgctcttcat cctgccggcc gt
//
LOCUS       DASUGJ010000202         9525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1001545, whole genome shotgun sequence.
ACCESSION   DASUGJ010000202 DASUGJ010000000
VERSION     DASUGJ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001545"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFV93_04410"
     CDS             <1..229
                     /locus_tag="VFV93_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164617.1"
                     /translation="LLTVASAENRVLVSYDTKTLPPLVKDCWERDLHHAGIILIDSRT
                     FRQNDIGGIVRALRTLVENRGVKTGMTGWSS"
     gene            402..1229
                     /locus_tag="VFV93_04415"
     CDS             402..1229
                     /locus_tag="VFV93_04415"
                     /inference="COORDINATES: protein motif:HMM:NF015402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HEX5164618.1"
                     /translation="MSANGQSGVLETLFGRSKPVIAMMHLLPLPGRPLYDRAGGVGAI
                     VESLKRDLDILQEGGVDGLLFCNEGDRPYPLATDRAPIAAMAAAIGELRPQIGLPYGV
                     DLLWEGMAAIAVAQAVQALFIREVFTGVYDSDMGLWQPDAAAALDYRERIGAGNIRLF
                     FNIEPEFARPVSPRPISALARSVVTSSLADALLISGPMAGAEADLTHIGEAKAAVPTT
                     PVLANTGCRVETVAQTLTVADGVIVGTGLKQDGYTWNPVDPDRVRRFMANAREARGD"
     gene            1358..1642
                     /locus_tag="VFV93_04420"
     CDS             1358..1642
                     /locus_tag="VFV93_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164619.1"
                     /translation="MTDWLAERVKRGIEIVVDAAAQASIRLKNISIFGTDYYDDSFRE
                     IFIWVYVDAPHSEAYDYWGAVAGPIHALNQPPPPRAQHADVRLEVSVDSL"
     gene            1639..2031
                     /locus_tag="VFV93_04425"
     CDS             1639..2031
                     /locus_tag="VFV93_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164620.1"
                     /translation="MNESEFDPLHFLRLADGLATDDADEAALRTAIGRAYYAVFLLAR
                     ARTGVQGKWQAHERVRQAISPVNDRLAAILGTIATVRYFADYELHITNANFSDWKRNW
                     QQVRQNVTRVLEELESLPDLTISENIDR"
     gene            2045..3538
                     /locus_tag="VFV93_04430"
     CDS             2045..3538
                     /locus_tag="VFV93_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX5164621.1"
                     /translation="MTARYLLGIDIGTTALKAVLLDPERGVVAQAGGSHTLRSPHPGW
                     AEMDPAEWWATTVAACRKLTRHVPAGEIAAVAVTGMVPAMVLLDAAGNVLRPSIQQND
                     ARSAVQVEELRRAVDLNAFFSITGGTPNQQNIAPRWRWLATHEPAVTSRCATLFGSYD
                     YITWLLTGGRSVELNWAAESGLFDITSRDWHVPYLEQAALSPGMLPGVLRPGERAGVV
                     TESAAAVTGLRAGTPVVCGGADHVGAALAAGMTQPGDLLLKFGGAGDILYVADRPDPD
                     PHFYFDFHDIPGLTLINGCMAASGSFVKWFATELGGGATLTELDHEAASVPPGSAGVV
                     ALPYLLGEKTPIFDPLACGVFAGVMLHHRQAHLYRAVLEAVCYGFRHHLELLQASGRT
                     VGRVLAADGGSRSALWMQIAADVTGQPVQVIAGEAASALGTAFVAGIGASACSGWHEI
                     ERFIERGPRYEPRPDAVAAYDSGYAIYRDLYTRLQPLFPSMARLDTA"
     gene            3634..4428
                     /locus_tag="VFV93_04435"
     CDS             3634..4428
                     /locus_tag="VFV93_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5164622.1"
                     /translation="MELGLNGKIAMVGGASKGIGFNVARLLAKEGVSVSISSRNEAAI
                     QAAKQQIEAETPGAQVLAVAADLSKAGDIDGWLSTTMERFGGVDLLFTNTGGPPGGTF
                     NDFGDTDWQAAFDLLVMSVIRMTRGVYPSMQARGGGAIVMSTSSSVKEPIANLTLSTV
                     LRASVSALAKSLANEWAGDRIRVNQIVPGRILTDRIRSMDQARSEKSGQPVEEIRRQS
                     EATIPLKRIGDPEEYASGAVFLFSDAASYITGATLQVDGGLIRSVT"
     gene            complement(4530..6356)
                     /locus_tag="VFV93_04440"
     CDS             complement(4530..6356)
                     /locus_tag="VFV93_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX5164623.1"
                     /translation="MAFASLHTARTVKMLAVVLAMVMLGLGIMQAFAVAPGTPTFERT
                     WARTDKPVVDGEADRTWMWGPEANTGVIPEAYAESPEGTRQVQYFDKSRMELTHPDAV
                     DDGVWYVTNGLLVNELISGRVQIGDELYQVREPASDINVAGDPNDPNGPTYGTIGKVL
                     DEPPLADGAPVTQRLARDGTISDDPSLADRGVTAAYHAQVTDIDHQIASPFWEFMTSQ
                     GTVYEDGEFVEDLLFQNAFYATGYPIAEPYWASVKLQDVVQDVLIQCFERRCLTYTPG
                     NEPGWQVEAGNVGQHYYRWRYPDIPTPTPTSTATGTTTPTATATETGTATGTTTATSS
                     VTPTPTTPTVPDDELFVANLSGVNASITGTGVTYFFVRGDGSGIDFQLLVTDLVDVTA
                     AHLHSNDGGINPPIIASLFTASGGTSVTPDGLLAEGTIEADDLPADMTLAELTSAIEA
                     GNIYVDVHTLLNTTGAIRGPVQPLARATLRAALKGENEIPPVLTNASGAALLTFDAED
                     RAFGFQVALTAVEGLTSVQIRVGAVNENGPVAATLYAVSTPQQYFNDVLTGTIRVDDI
                     QGMTMERLVYLMLTGDAYVNVHTVFNPDGLIRGQLGPSLGGM"
     gene            complement(6605..>9525)
                     /locus_tag="VFV93_04445"
     CDS             complement(6605..>9525)
                     /locus_tag="VFV93_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5164624.1"
                     /translation="NLVLFSIQDYVASPGGDHGQLCASGVAGQGVTNAPSALDGSYPS
                     YKPEGTSGMVTAAGKDVELINAGDFYSMLGTDAFYFAALGYDGTPGTEDDTQIISNSW
                     GFSDIHNDGWDFMSREIDAIVRFLNPTLLVLGSSGNGAPGFGTTGQPAPAAGMSVGAS
                     TEYGSTGIFDSIVSADQITYGDVVSWSGRGPGARGDAGVSILGNGSVGSGAMPLNEMF
                     SGASAWETWSGTSRSTPVVAGNAALMYQAYMEANGSWPDFETAKAILMSGARDLHYDP
                     FLQGTGSVNASRAVDLASGAAGSYVTPSDWIAGSWHGEEYPAFTNIMKPGETDDAVFE
                     VHNPSDADLHLTVGSRWLLKQGEWTNQFTSAPVSQEPIEFEEDDPVGAATDLNTPHYL
                     WNITDLVPESTDVLVARINVPYDQFDPAGSYDATQINIWRVYAYDWTDVNGDGNLWTD
                     DNGDGVVQDDELDDGEYIRFNYGYNASQDNTITVQSPRERATDGIFLGLIHRQARVEV
                     PQTNVSMLMEFYEQSNWDWLTTYSDELTVPAGGSNRINARMTVPGNAPHGAYEGALWI
                     NDGTWTSTIPVTVNVAGASNIEAGTSDDDTLGVTPYYRNDQVYGAQAWNWRQESGDWR
                     FYFADLSATPGGLGQPDQGQRYFMVDSQWEHMPSDINVHIFGPTLDCFTGAECEMPFD
                     PYDEATYGPYTLERLGGSDEYYIGSGVFLTATTSGANREFVAAPYVPGLNEVMLDNVN
                     FASVEQAESFGMRAGALSVSVAPIDVSRPASDPSYLLQEQVVSTMDLSGLVVQGFGLS
                     RPEVKTDLPIQQDDPNDPFTSSHIEEFTVGNAGLLDIATDGQDGDDIDLYLLYDFNGD
                     GEFSGDELVGSSTTPTADEHIRITLPAAGEYQLLVHGWSIPAGDSTFDLSINIVQGDD
                     ISVSGLPEGAITPGVRYNFAIEFNPEGVAPGTYEGLITLGPPEGPAAILIPVTYEIRE
                     "
BASE COUNT         1629 a         3045 c         3162 g         1689 t
ORIGIN      
        1 ccttctgaca gtcgcctcgg cagagaaccg cgtgctcgta tcctatgaca cgaagaccct
       61 gccaccactg gtgaaagatt gctgggaacg agatctgcat cacgcgggga ttatcctcat
      121 cgatagccgg acgtttcggc agaacgacat cggtgggatc gtgcgcgccc tgcgtacgtt
      181 ggtcgagaat cgcggggtga agactggcat gaccggatgg agttcctgaa ccgctgatct
      241 cgcagccata tgagccagag gaacgaccag ggatattcgc gtccctggtc gtcggcgtgt
      301 cgatggtgtg tgagaggcga gtggcggtat caggcgaaac aggcggtaca ctgtccagcg
      361 attggcattt gaacccgaca agaacaggaa cgatgtgtcg catgtctgca aacggtcaat
      421 ctggtgttct cgaaacgttg ttcggacggt cgaagccggt cattgcgatg atgcacctgc
      481 tgccgctgcc gggccggccg ttgtacgacc gggctggcgg ggtcggggca atcgtcgaga
      541 gcctgaagcg cgatctggac atccttcagg aaggcggcgt cgacggactg ctattctgca
      601 atgagggcga ccgcccctat ccgctggcga ccgaccgggc gccgatcgcc gcgatggccg
      661 ccgcgatcgg cgagctccga ccccagatcg gcctgcccta cggcgtcgat ctcctctggg
      721 aggggatggc cgccatcgcc gtcgcccagg ccgtccaggc gttgttcatc cgcgaggtct
      781 tcaccggcgt ctacgacagc gacatgggtc tctggcagcc ggacgccgcc gccgccctcg
      841 actatcgcga gcggatcggc gccgggaaca ttcgcctgtt cttcaacatc gagccggagt
      901 tcgcccggcc cgtctcacca cgcccgatca gcgcgctggc gcggagcgtc gtcacctcgt
      961 cgctggccga cgcgttgctg atctccggac cgatggccgg cgccgaggcc gacctcaccc
     1021 atatcgggga ggccaaagcc gccgtcccaa ccacgcccgt tctcgccaat accggctgcc
     1081 gggttgagac ggtcgcccag acgctgacgg tcgccgatgg cgtgatcgtc ggcaccggcc
     1141 tcaagcagga cggctacacc tggaacccgg ttgaccccga ccgcgtccgc cgcttcatgg
     1201 ccaacgcccg cgaagcacgc ggggactgag atcgtcacag gctatacttg cttcagtcgg
     1261 cattggagca gcgacgatga aagaacgcag cgtgagccag tcgatcgcac cgccaaccac
     1321 agtcccgatt gacattcagc cgtctgctga ggaactgatg actgactggc tggctgaacg
     1381 cgtgaagcga ggtatcgaga tcgttgtgga cgccgcagct caagccagta tccgcttgaa
     1441 gaacatttcg atcttcggaa ccgactacta cgacgattcc ttcagggaga tcttcatctg
     1501 ggtctacgtc gatgctcctc actccgaggc gtacgactat tggggagctg ttgccggccc
     1561 gattcacgca ctcaaccagc caccgccacc tcgggcacag catgctgatg tcagattaga
     1621 ggtgtcggtt gactcgttgt gaatgagtca gagttcgacc cgctgcactt cctccgcctg
     1681 gcggacggtc tagcgacaga cgacgcggac gaagcagcgt tgcgaacggc tattggccgt
     1741 gcgtattacg ctgtgtttct gctggcacga gccaggacag gcgtacaggg aaagtggcag
     1801 gcacacgagc gagtgcgcca ggcgatcagc cccgtcaacg accggttagc ggccatactc
     1861 gggacaattg ccaccgtccg gtatttcgcc gactacgaac tacacattac gaacgccaat
     1921 ttctccgact ggaaacgcaa ctggcaacag gttcgccaga acgtcacacg tgtactcgag
     1981 gaactcgaat cccttccgga cctcacgata tcagagaaca tcgatcgctg aggaacaacc
     2041 tcgcatgaca gcacgctacc tgctcgggat cgacattggg acgacggcgc tgaaggcggt
     2101 gctgctcgat ccagaacgtg gtgtcgtcgc tcaggctggc gggtcgcata ccttgcgctc
     2161 gccgcacccc ggctgggccg agatggaccc ggccgagtgg tgggcaacga ccgttgccgc
     2221 ctgccgcaag ctgactaggc acgtcccggc cggcgagatc gcggccgtcg cggtgaccgg
     2281 gatggtgccg gcgatggtgc tgctggacgc cgccggcaac gtcctgcggc cgagcatcca
     2341 gcagaacgac gcccgatcgg ccgtgcaggt tgaggaacta cgcagggccg tcgacctcaa
     2401 cgcgttcttc tccatcaccg gtgggacgcc caaccagcag aacatcgccc cacgctggcg
     2461 ctggctggcg acgcacgagc cggcggtgac cagccgctgc gcgacgctgt tcggctccta
     2521 cgactacatc acctggctgc tgaccggcgg gcgctcggtg gagttgaact gggccgccga
     2581 gagcgggctg ttcgacatca ccagccggga ctggcacgtc ccgtatctgg agcaggccgc
     2641 tctctcgcca ggtatgctgc caggcgtgct ccggccgggc gaacgggccg gtgtcgtcac
     2701 cgagtcggcc gccgccgtga ccggcctgcg ggctggaaca ccggtcgtct gcggtggtgc
     2761 cgaccatgtc ggggcggctc tggcggccgg catgacgcag cccggtgact tgttgctcaa
     2821 gttcggcggg gccggtgaca tcctttatgt cgccgatcgg ccggaccccg acccgcactt
     2881 ctacttcgac ttccacgaca tccccggcct gacgctgatc aacggctgca tggcggccag
     2941 tggctccttt gtgaaatggt tcgcaaccga gttgggcggt ggcgcgacac tcacggagtt
     3001 ggaccacgag gcggcatcag tcccgcccgg ttccgccggc gtcgtcgcgc tgccgtacct
     3061 gctgggcgag aagacgccga tcttcgaccc gctggcatgc ggcgtctttg ccggtgtcat
     3121 gctgcaccat cgccaggcac acctctaccg agccgtcctc gaagcggtct gctacggctt
     3181 ccggcatcac ctggaattac tgcaggcatc aggaaggaca gtcggccgtg ttctggcagc
     3241 ggacggtggc agccgcagcg ccctctggat gcagatcgcg gccgacgtga ccggccagcc
     3301 ggttcaggtc atcgccgggg aagcagcctc ggcgctcggc acggccttcg tcgccgggat
     3361 cggggccagc gcatgcagtg gctggcacga gattgaacgc ttcatcgaac gcggcccgcg
     3421 ctacgagccg cgccccgacg ccgtcgctgc ctacgacagc ggctacgcca tctaccgcga
     3481 cctgtatacg cgactccagc ctctcttccc gtccatggcc aggctcgata ccgcctgatc
     3541 catgcgatga tcacgccgga ccgtggacac gctcccactg gcgccaactg gtcggctacc
     3601 gagtcacagc atcgggaaag gagatagtgg tctatggaac tcggactcaa tggcaagatc
     3661 gcgatggttg gtggggcgag caaggggatc gggttcaatg tggcccgcct gctcgcgaag
     3721 gagggcgtta gcgtctcgat ctcgtcgcgc aacgaggcgg cgatccaggc cgcgaagcag
     3781 cagatcgagg cagagacacc cggtgcgcag gtgctggcgg tcgcagctga cctcagtaag
     3841 gccggtgaca tcgacggctg gctcagcacg acgatggagc gcttcggtgg cgtcgacctg
     3901 ctcttcacca acacgggcgg gccgccagga ggcacgttca acgacttcgg cgataccgac
     3961 tggcaggccg cgtttgacct gctggtgatg agcgtcatcc ggatgacgcg aggcgtctac
     4021 ccgtcgatgc aggcgcgtgg cggcggcgcg atcgtcatgt cgacctcgtc gtcggtcaag
     4081 gagccgattg cgaacctgac gctctcgacc gtcctgcgcg ccagcgtctc ggcactggcg
     4141 aagtcgctgg cgaacgagtg ggcgggcgac aggatccgcg tcaaccagat cgtccccggc
     4201 cggatcctga ccgaccgcat tcgctcgatg gatcaggcac ggtccgaaaa gtcagggcaa
     4261 ccggtcgaag agatccgccg ccaatctgag gcaaccatcc cgctcaagcg gatcggcgac
     4321 ccggaggagt acgccagcgg cgccgtcttc ctcttctccg acgccgcctc ctacattacc
     4381 ggcgcgaccc ttcaggtcga tggcgggctg atccgcagcg ttacgtaggt gataggtgat
     4441 aggtgttagg ggtgggttcg cgcaggagga cggcgtctcg ctcacccgca caaacccacc
     4501 cctaacacct accacctatc acctccgcgt cacatgcccc cgaggctcgg gccgagctgg
     4561 ccgcgaatga ggccgtcggg gttgaagacg gtgtggacat tgacgtaggc gtcgccggtc
     4621 agcatgagat agaccagccg ttccatcgtc atgccctgaa tgtcgtcgac ccggatggtg
     4681 ccggtcagga catcgttgaa gtactgctgc ggcgtgctga cggcatagag cgtcgcggca
     4741 accgggccgt tctcgttgac cgctccgact cggatctgga cgctggtcag tccttcgaca
     4801 gccgtcagcg cgacctggaa gccgaacgcg cgatcctcag catcaaaggt aaggagcgcg
     4861 gcgccgctcg cgttggtcag caccggcggg atctcgttct cgcccttgag cgccgcccgg
     4921 agggtcgccc tcgccagcgg ctgaaccggt ccacggatcg cgccggtcgt gttgagcagc
     4981 gtgtgcacat cgacgtagat gttgccggcc tcgatcgcgc tggtcagttc ggcaagcgtc
     5041 atatcggccg gcaggtcgtc ggcctcgatc gttccttcag ccagcagacc atccggcgtc
     5101 accgaggtcc cgccgctggc cgtgaagagc gaggcgatga tcggcgggtt gatgccgcca
     5161 tcgttgctat ggagatgggc ggccgtgacg tcaaccaggt ccgtcacgag caactggaag
     5221 tcgatgccgc tgccgtcgcc acggacgaag aagtaggtca cgccggttcc ggtgatcgag
     5281 gcgttgaccc cgctcagatt ggcgacgaac agctcgtcgt cgggaaccgt cggcgtcgtc
     5341 ggggttggtg tcaccgacga tgtcgccgtg gtggtgccgg tggcagttcc ggtttcagtc
     5401 gcggtcgcgg ttggggtcgt cgtgccggtg gcggtgctgg ttggtgttgg tgtcgggata
     5461 tcgggatagc gccagcggta gtagtgctgg ccgacgttgc cggcctcgac ctgccagccg
     5521 ggctcgttgc cgggggtgta ggtcaggcag cgccgctcga agcactggat caggacgtcc
     5581 tgaacgacat cctgcagctt gacgctggcc cagtagggtt cggcaattgg gtagccggtg
     5641 gcgtagaacg cattctggaa gaggagatcc tcgacgaact cgccgtcctc atagacggtc
     5701 ccctgcgagg tcatgaactc ccagaacggc gacgcgatct ggtggtcgat atcggtgacc
     5761 tgtgcgtggt aggcggctgt gacgccccga tcggcgagcg acggatcatc gctgatcgtc
     5821 ccatcgcggg ccagccgctg ggtgaccggc gcgccatcgg ccagcggcgg ctcatcgaga
     5881 accttgccga ttgtgccgta ggtcgggccg ttgggatcgt tgggatcgcc ggccacgttg
     5941 atatcgctgg ccggctcgcg aacctggtac agctcgtcgc cgatctggac ccggccggag
     6001 atcagctcgt tgaccagcag gccgttggtg acataccaga caccgtcatc gacggcgtcc
     6061 gggtgggtga gctccatccg gcttttgtcg aaatactgaa cctgccgcgt cccctccgga
     6121 gactcggcat aggcttcggg aatgacaccg gtattggctt ccggccccca catccaggtc
     6181 cggtcggctt cgccatcgac gaccggcttg tcggtccgtg cccacgtgcg ctcgaaggtg
     6241 ggagtgccgg gtgcaacggc aaacgcctgc atgatcccca gaccaagcat gaccatcgca
     6301 agcacgacag ccagcatctt gacggtacgc gcggtgtgca gactggcaaa cgccatcgtc
     6361 gccccccttc gctcactcca tgtaccccgg agtcagtatc cgaacccatc ccatcccaca
     6421 cggccggatc acggtcatcc ccttgaccgc gataccttgt gggacgctat ccatcataac
     6481 ccgccagccg gaatgccgcg agatttcgtg acagccagtc acagaacgga gcgaggcgtg
     6541 agacgcgaac ggggagggtc ttgcgaccct ccccgtcgag ccttacagct tgtgtggcgc
     6601 tcgcctattc ccgaatctcg taggtgaccg ggatcaggat cgcggccggg ccttccggcg
     6661 ggccgagcgt gatcaggccc tcgtaggtgc ctggcgcgac gccctccggg ttgaactcga
     6721 tcgcgaagtt gtagcgcacg cccggggtga tcgcgccttc cggcaggccg gagacactga
     6781 tgtcgtcacc ctggacgatg ttgatcgaaa gatcgaacgt cgaatcaccg gccgggatcg
     6841 accagccgtg gaccagcaac tggtattcgc cggccgccgg cagggtgatc cgaatgtgct
     6901 cgtctgccgt cggcgtcgtc gaggagccaa ccagctcgtc gccgctgaac tcgccgtcgc
     6961 cgttgaagtc gtacagcagg tagagatcga tgtcgtcgcc gtcctggcca tcggtcgcga
     7021 tgtccagcag gccggcgttg ccgaccgtga actcctcgat gtggctggac gtgaacggat
     7081 cgttcgggtc atcctgctgg atcggcagat ccgtcttgac ctcgggccgg ctcaggccga
     7141 agccctggac gaccagaccg gagaggtcca tcgtcgagac gacctgttcc tggagcaggt
     7201 agctcgggtc actcgccgga cgcgagacat cgattggcgc gaccgagacg gagagtgcgc
     7261 cggcccgcat gccgaacgac tctgcctgct cgaccgaggc gaagttgacg ttgtcgagca
     7321 tcacttcgtt gaggcccggc acatacgggg ccgccacgaa ctcgcggttc gcgccactgg
     7381 tcgtcgccgt caggaagacg ccgctgccga tgtagtactc gtcgctgccg ccgagccgtt
     7441 cgagcgtgta cggcccgtag gtcgcctcat cgtacgggtc gaatggcatc tcgcattcag
     7501 cgcccgtaaa gcagtccagc gttgggccga agatgtggac attgatatcg ctgggcatgt
     7561 gctcccactg cgaatcaacc atgaagtagc gctggccctg gtccggctgg ccgaggccgc
     7621 cgggggtcgc ggacaggtcg gcgaagtaga agcgccagtc gcccgactcc tgccgccagt
     7681 tccaggcctg cgccccgtag acctgatcgt tgcggtagta cggcgtcaca cccagcgtgt
     7741 catcgtcgga cgtgccggcc tcgatgttgg atgcacccgc gacgttgacc gtcaccggga
     7801 tcgtcgaagt ccaggtgccg tcgttgatcc agagcgcgcc ctcgtaggcg ccgtgcggcg
     7861 cgttgcccgg caccgtcatc cgggcgttga tgcggttcga gccaccggcc ggaaccgtca
     7921 gctcatcgct gtaggtcgtc aaccagtccc agttggactg ctcgtagaac tccatcagca
     7981 tgctgacgtt ggtctgcggg acctccacgc gtgcctgtct gtggatcagg ccaaggaaaa
     8041 taccgtcggt cgcgcgctcg cgggggcttt ggacggtgat cgtgttgtcc tggctggcgt
     8101 tgtagccgta gttgaagcgg atgtactcgc cgtcgtcaag ctcgtcgtcc tgaacgacgc
     8161 cgtcgccgtt gtcgtccgtc cagagattgc cgtcgccgtt gacatccgtc cagtcgtagg
     8221 cgtagacacg ccagatgttg atctgagtcg cgtcgtagga gccggccgga tcgaactggt
     8281 cgtaggggac gttgatccgc gcgaccagca cgtcagtcga ttccggcacc agatcggtga
     8341 tgttccagag gtagtgcggc gtgttgaggt ccgttgccgc cccaaccggg tcgtcctcct
     8401 cgaactcgat cggctcctgg ctgaccggcg ccgaggtgaa ctggttggtc cattcgccct
     8461 gcttgagcag ccagcgcgag ccgaccgtca ggtgcaggtc cgcgtcgctc gggttgtgga
     8521 cctcgaagac ggcgtcgtca gtctcacccg gcttcatgat attggtgaag gccgggtatt
     8581 cctcgccgtg ccacgagccg gcgatccagt cgctcggcgt gacgtaggaa ccggcggcgc
     8641 cggaggcgag atcgaccgca cggctggcgt tgaccgagcc ggtgccctgc aggaacggat
     8701 cgtagtgcag gtcccgcgcg ccgctcatca ggatggcctt ggccgtctcg aagtccggcc
     8761 agctgccgtt ggcttccatg taggcctggt acatcagggc agcgttgccg gcgacaaccg
     8821 gcgtcgaccg gctggtgcca gaccaggtct cccaggcgct ggcgccggag aacatctcgt
     8881 tcagcggcat cgcgcccgag ccgactgagc cattgccgag gatgctgacg ccggcgtcac
     8941 cgcgcgcgcc ggggccacgg ccggaccacg agacgacatc gccgtaggtt atctgatcgg
     9001 cactgacgat cgagtcgaag atgccggtcg agccgtactc ggtcgaggcg ccgaccgaca
     9061 tcccggcagc cggggccggc tggccggttg tgccgaagcc cggcgcgccg ttgccggacg
     9121 agccgaggac cagcagggtc gggttcagga agcgcacgat cgcgtcgatc tcgcgcgaca
     9181 tgaagtccca accgtcattg tggatgtcgg agaagcccca cgagttggag atgatctggg
     9241 tatcgtcctc ggtgccgggc gtgccatcgt agccgagggc ggcgaagtag aaggcatctg
     9301 tgccgagcat cgagtagaag tcgccggcgt tgatcagctc gacgtccttg ccggcggcag
     9361 tgaccatccc gctcgtgcct tcgggcttgt agctcgggta ggagccatcc agcgccgatg
     9421 gcgcgttggt gacaccctga ccggcgacgc ccgaggcgca gagctggccg tggtcgccgc
     9481 cggggctggc gacgtagtcc tgaatcgaga agaggacgag gttgc
//
LOCUS       DASUGJ010000203         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1139961, whole genome shotgun sequence.
ACCESSION   DASUGJ010000203 DASUGJ010000000
VERSION     DASUGJ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139961"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..942
                     /locus_tag="VFV93_04450"
     CDS             268..942
                     /locus_tag="VFV93_04450"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5164625.1"
                     /translation="MFSGLDERVIDIIERFGYAGIGLLIALETIFPPLPSELILPLGG
                     FTASRGDLTLWGVMLAATLGSVAGALVLYGVGHWFGRERLYWLVDRYGRYLMLKQSDL
                     DRTFDVFERYSGKAVMFGRLIPVVRSLVSLPAGLTRMPLPAFILYTAIGSAVWNGILV
                     GAGYILGDQWDKVESVVGYLQYAVILAAIAGAGWFLWNRLRGKKDADLRRSSPRAHST
                     TIEPED"
     gene            1019..1330
                     /locus_tag="VFV93_04455"
     CDS             1019..1330
                     /locus_tag="VFV93_04455"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX5164626.1"
                     /translation="MDNVIEKHIEIIEGYGGPQTRIVGHRINVSHIVMYYKEHGLSPA
                     EIVEQLPTITLADVHAALAYYYDNPDLIAAEEAAEARYIEEIKQQYPSRLHEMLQHRS
                     G"
     gene            1323..1655
                     /locus_tag="VFV93_04460"
     CDS             1323..1655
                     /locus_tag="VFV93_04460"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX5164627.1"
                     /translation="MADIRFQLDEHIEAAVAIQLLQRGVDVLTADDAGLRRAPDTAHF
                     SHSRKTGRVIVTYDHHFAGMHQRGVEHAGIVYFPKGRRSVGEIVEALVLVHGVYTPEE
                     MINRIEFF"
     gene            1935..>3096
                     /locus_tag="VFV93_04465"
     CDS             1935..>3096
                     /locus_tag="VFV93_04465"
                     /inference="COORDINATES: protein motif:HMM:NF021352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2357 domain-containing protein"
                     /protein_id="HEX5164628.1"
                     /translation="MSPIDVGVFQVRFENQLGLASIQPLDEHGPCCSPLVLEVISPKF
                     PSIEQHLAFLTALLDDLFARAARLPFAISAETTRGVVDVPTPPTPLFTLHFLLQHAQS
                     LREAAQAVLSRPHRRLDDTPETVPLASVSEADPDVLLDVLTSPERWVRASGFPLATRL
                     CGHAPAEVWQRRPEETLDTPENRFVKAFLSDLLFAAERLPTQPWWRHVSTERRRVIHE
                     TEAMLRLTLAQPTFAEVGDLRRIPSASRVLLRREGYRELLDSWRLFNLARRPFFGPLQ
                     RAIDLRDIATLYEAWCFFALVEGIAAALGIEPALSLRVSDEHGLNWRSEARFGSTGTL
                     VYNQSVRGYSVGLRPDYLWYRDGKPDVALDAKFRLDRADWNDDTSHATVKNDD"
BASE COUNT          538 a         1012 c          938 g          608 t
ORIGIN      
        1 acggtcagcc atcaacgatc agtcatctgc ctttcagtca gccagccggt caacgatgct
       61 acaggagcct gactcgatgc tggtggggca ccatcatacc cgcctgccac ctgagacgcc
      121 gatggctgat cggccgccaa ctgatacgct ttgcgttcgt gttccccggc agttggtccg
      181 gggcaggcta acagacagac gacagacgac agacggccac cggcagcgcg ccgtcggctg
      241 atagattgca gactgacagg cgacatcatg ttttctggac tggacgaacg ggttatcgac
      301 atcatcgagc ggttcggcta tgccgggatt gggctgctga tcgcgctcga aacgatcttc
      361 ccaccgctgc cgtcggaact gatcctgccg ctgggcgggt tcacggcctc gcggggtgat
      421 ctgacgctct ggggcgtcat gctggcggcg acgctgggtt cggtcgccgg cgcgctggtg
      481 ctgtacggcg tcggccactg gttcgggcgc gagcggctct actggctggt cgatcgctat
      541 ggccgctacc tgatgctgaa gcaatccgac ctcgacagga cattcgacgt cttcgagcgc
      601 tactccggca aggctgtcat gttcggccgg ctgatcccgg tcgttcgcag cctcgtctcg
      661 ctgccggccg ggctgacgcg gatgccactg ccggccttta tcctctatac cgcgatcggc
      721 agcgccgtct ggaacggcat cctggttggc gccggctaca tcctcggcga ccagtgggac
      781 aaggtcgaaa gcgtcgtcgg ctacctgcaa tacgccgtca tcctggccgc catcgccggc
      841 gccggctggt tcctctggaa ccgcctgcgg ggaaagaagg acgctgacct caggcgcagc
      901 agcccgcgcg cccactcgac gacaatcgag ccggaagact gagcgccgat cacgcctcat
      961 ctcctgcttg cgctatctgc atacccttcg tatcatctgt tcagaaggtg gtgttgccat
     1021 ggacaacgtg attgagaagc acattgaaat catagaaggt tatggaggtc cgcagacaag
     1081 aattgttggc caccgaatca atgtgtcaca tatcgtcatg tattataaag aacatgggtt
     1141 gtccccagcg gaaatcgttg agcaattacc gacgatcacg ttggctgatg tccacgctgc
     1201 gctggcgtac tactatgaca atcctgatct gatcgctgct gaagaagccg ctgaagcacg
     1261 ctacatcgaa gagattaagc agcagtaccc cagccgactc cacgaaatgc ttcaacaccg
     1321 tagtggctga catacggttt caacttgatg agcacattga agctgctgtc gcgatccagc
     1381 tcctccagcg tggagtcgat gtgctgactg ccgatgatgc ggggttgcgg cgggccccag
     1441 acacggccca cttttcgcat tcgcgaaaga ctggtcgagt gatcgtcacc tatgaccacc
     1501 atttcgctgg aatgcatcag cgcggcgttg agcacgcggg cattgtgtat ttcccaaagg
     1561 gacgccgaag tgtcggtgag atcgtcgaag cgctcgtatt ggttcacggt gtctacactc
     1621 cggaagagat gatcaatcgc atcgagttct tctgacaggc tccgctgttc ctggattggt
     1681 cgatgatggc ttctcccacg atccaaccga ggcctggcaa tccttgattc tgaaggacat
     1741 cattcatcga cagcgacacc atcgtcccat ttgccgacgc gctcggctgg ctccggcccg
     1801 ttccgctggg tcggccgatc gtcgcgctag ttgacgaccc gcgcctggcg ccggcgacgc
     1861 acgcggtcta cgagtggtgg gactacgtcg ttcactacga cggcgcgacg agcttacggg
     1921 tcagcgatcg gctggtgtcg ccgattgatg ttggcgtgtt tcaggtccgc ttcgagaatc
     1981 agctcgggct ggccagtatc cagccgctcg acgagcacgg cccatgttgc tcgccgctgg
     2041 tgctggaggt gatctcgccc aagttcccga gtatcgagca gcacctcgcc tttttgacgg
     2101 cgctgctgga tgatctcttc gcgcgggccg cccggctgcc gtttgcgatc agcgccgaga
     2161 cgactcgcgg tgtcgttgac gtgccaaccc cgccaacgcc gctcttcaca ctgcacttcc
     2221 tgctccagca cgcgcagtca cttcgcgagg cggcccaggc cgtcctgtcc cgtccacacc
     2281 ggcggctgga cgacacgccg gagaccgtgc cgctggcgtc tgtcagcgag gccgatcccg
     2341 atgtgctgct ggacgtgctg accagcccgg aacgctgggt ccgggccagt ggcttcccac
     2401 tggcgacgcg gctttgcggc cacgcgccgg ccgaggtctg gcagcgccgc cccgaggaga
     2461 cacttgacac cccggagaac cgcttcgtca aggcgttcct gagcgacctg ctcttcgccg
     2521 ccgagcgact gccaactcag ccgtggtggc ggcacgtctc caccgagcgc aggcgagtca
     2581 tccacgagac ggaggcgatg ctgcggctga cgctcgccca gccgacgttc gccgaggttg
     2641 gcgacctgcg ccggatcccg tcggcctcgc gcgtcctcct gcgccgcgag ggctaccgcg
     2701 aactcctcga cagctggcgg ctcttcaacc tggcccgccg tccgttcttc ggcccgctcc
     2761 aacgcgccat cgacctgcgc gacatcgcga cgttgtatga ggcttggtgt ttcttcgcgc
     2821 tggtcgaggg gattgccgca gctctcggca ttgaaccggc gctctcgttg cgcgtatccg
     2881 acgaacacgg cctcaactgg cgctccgaag cccgcttcgg ctcgaccggc acgctcgtct
     2941 acaaccagtc ggtacgcggc tactcggtcg gcctacgccc ggactacctc tggtaccgcg
     3001 acggcaagcc cgacgtcgcc ctcgacgcca aattccgcct cgaccgcgcc gactggaacg
     3061 acgacacgtc gcacgctacc gtcaagaatg atgacc
//
LOCUS       DASUGJ010000204         7359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1835169, whole genome shotgun sequence.
ACCESSION   DASUGJ010000204 DASUGJ010000000
VERSION     DASUGJ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835169"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2396
                     /gene="recG"
                     /locus_tag="VFV93_04470"
     CDS             <1..2396
                     /gene="recG"
                     /locus_tag="VFV93_04470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX5164629.1"
                     /translation="LLGASDDTTRTQVQELDALLADYANLPMGERARSVGDARSIVAG
                     LMQRSPNGRTPVAPPQRQVATPRPARQAPAQASTVSPAATPRPVAATTPTRPAPAKLP
                     TGRISSLSDTVRLLPGVGEARAKLLEQLGVRTVRDLLRLYPRRHIDYSNVQKIGSLLF
                     GHISTIQGTVQSIESSRTRTGKQLFDVVVDDGTGRIHAAFFNPWIERQLRPGVPVSLS
                     GRIEQMRGNLCLSNPEWEVLDHETINTGRMIPVYPLTKGLYQKTLRALVRYALDGAGH
                     LLEDYIPDDLRRQEGVIGLVEATEWIHFPDGETPVEAQRRLDAARGRLAFDEFLVLQL
                     GLLQRKQDWQSQPGTRIEIDREALDRFGASLPFAMTGAQQRALREILGDMAAPRPMTR
                     LLQGDVGSGKTIVAALAALAAVRAGYQVALMAPTELLAEQHSRGLRTVFDSGPEDLRP
                     RLAMLTGSVGGTEREMIYEATADGRLDLIVGTQALIQSGLEFRRLGLAIIDEQHRFGV
                     EQRASLRGKGESPDLLVMTATPIPRTLALTLHGDLDVSTLDELPPGRQPIETYWASEA
                     KRAGAYSFVREQVAQGRQAFIVFPLVEESEAIDARAAVAEHERLAAEVFPDLRLGLLH
                     GRMKPAEKDAVMLAFRDREIDILVSTSVVEVGIDVPNATVMLIDGAERFGLSQLHQFR
                     GRVGRGPEKSYCILLSTDPSIESKQRLQAMVDSQDGFRLAQIDLDLRGPGDFLGKRQS
                     GLPEMELASFADVRDLERARMAAERVLAEDPKLDSAKYALLKARVDAFWQTAIADVS"
     gene            2400..3005
                     /locus_tag="VFV93_04475"
     CDS             2400..3005
                     /locus_tag="VFV93_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEX5164630.1"
                     /translation="MRVVSGEARGRKLRAPKSQGITRPMADKIKEALFSVLASLGVEY
                     DRVLDLYAGSGGVGIEALSRGASWCDFVDRDTHAVRAIRENLEHVGFADRARVHQVPV
                     LSAIHGAHEPYDMVLFDPPYADPDIISTLNALSFSHAVRDGTIVAVGHSPRVDLPERI
                     GRLEKLRGRCHGDSCFSVFDVVLDAGEDAAASVQPASSHEE"
     gene            3016..3558
                     /gene="coaD"
                     /locus_tag="VFV93_04480"
     CDS             3016..3558
                     /gene="coaD"
                     /locus_tag="VFV93_04480"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX5164631.1"
                     /translation="MRVAVYPGTFDPITNGHIDLAKRAARLFDRLIVAVYSGGDRAPK
                     RPLFTTEERVAMARQSLSDIPHVEIDTFENLTVDYARSVGAQTIVRGLRAVSDFEYEF
                     KLAHMYQHLAPELEVVCLMTSSAHSFISSSFIREVASLGGDIDDLVPAHVAEALSMRF
                     DAGQLQSDGNGLLVRTQGGM"
     gene            3600..4070
                     /locus_tag="VFV93_04485"
     CDS             3600..4070
                     /locus_tag="VFV93_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164632.1"
                     /translation="MGYETQTHGRDMMDLLDRLESQVADGRRVLLSNRIMVEAEEFMA
                     LLDQLRDCIPAEIQQARRVLQERQKIIVEAQTEATKIITMAKERSEYLVSQHGVTAEA
                     RYRGEDSLREAREKANRSMSAIDKYARERLETVEKVIRDNLAEIERAKGSLSQV"
     gene            4278..4805
                     /locus_tag="VFV93_04490"
     CDS             4278..4805
                     /locus_tag="VFV93_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX5164633.1"
                     /translation="MDLKNDTVINVAQLMKEDIGAARKVHMSLDWFALDQDLMAKDLS
                     ADLRLTRITNGILASGHVAGIAMVECVRCLELYDQPFSADFDQEYRPTIDVRSGVPLD
                     QPAADEELGTIDASHELDLAEPIRQVAILDLPIKPVCHDDCAGVGQSEFGDEDEGDRR
                     LGVLSQLLEGDDEPS"
     gene            4873..5073
                     /gene="rpmF"
                     /locus_tag="VFV93_04495"
     CDS             4873..5073
                     /gene="rpmF"
                     /locus_tag="VFV93_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX5164634.1"
                     /translation="MGALPKQRTTRAKQGFRRQHDKLTPVHLMTCPQCRQYKRQHHVC
                     PSCGYYAGRQVLEIEVKRRRES"
     gene            5196..6206
                     /locus_tag="VFV93_04500"
     CDS             5196..6206
                     /locus_tag="VFV93_04500"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX5164635.1"
                     /translation="MRHAAVTGWGMAVPDRVLTNHDLSQMVDTSDEWIVSRTGIRERR
                     IVSAGDTATSMSVTAGRAACEKAGVDPAQLDLIIVGTYTADHQMPSVASLVQNELGAR
                     RAGAFDLNAACSGFVYALAVGSQFIQTGVYSKVLVIGTDLASRFLDYTDRGTCILFGD
                     GSGAVVLQPSEANEGVLSIALGSDGSAGHHLTLGDNTGLTNLNGSRTTERPFMKMNGP
                     EVFRFAVKVMGEVAAEAVIKAGLTFGDVDLLVPHQANLRIIATAAKRLELPRERVWVN
                     VDRYGNTSSASVPISLVEAVDAGAIQDGMNVVVVGFGGGLTWAAGVLRWGSSGVAQGD
                     RR"
     gene            6203..7123
                     /gene="fabD"
                     /locus_tag="VFV93_04505"
     CDS             6203..7123
                     /gene="fabD"
                     /locus_tag="VFV93_04505"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5164636.1"
                     /translation="MTAGAAWIFPGQGSQSVGMGRDLAARYPEAQSAFDEADAALGFS
                     VSSLCWDGPDTELTRTANQQPALLTVSIATLRVLLKEDLLPEPDYVAGHSLGEYSALV
                     AAGALSFADALRLVRRRGELMEQYGRGGMVAVIGLDEEAMQLVANESGAEVANFNAPG
                     QTTLSGSDEALEVAEIAAKTRGARRVIKLPVNGAFHSSLMMPVADELYRDIVQTEIVK
                     PRVPLVSNVTGEPIANPDDIRNELVEQICASVRWTRGIETMIELGVTHFYEIGAGNVL
                     TGLVGRIDKSATALASDRLLGNRLKNGGGA"
     gene            7120..>7359
                     /locus_tag="VFV93_04510"
     CDS             7120..>7359
                     /locus_tag="VFV93_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5164637.1"
                     /translation="MSETSERRAAIVTGATRGIGRAIALQLATEGFNIVVNYRGEEAS
                     ATSLVDELQAHGVEARAIRADITSADDVAGLIEGTI"
BASE COUNT         1278 a         2293 c         2540 g         1248 t
ORIGIN      
        1 ggctgctcgg tgcgtcggat gacacgacgc gaacgcaggt ccaggagctt gacgcactgc
       61 tggctgacta cgccaacctg ccgatgggcg agcgggcacg aagcgttggc gacgcacgca
      121 gcatcgtcgc gggtctgatg caacgcagtc cgaacggacg caccccggtc gcaccacccc
      181 agcgtcaggt cgcaacgccg cgtccggcac gccaggctcc ggcacaggca agcacggtat
      241 ctcccgcagc gacgccgcgt ccggtcgcag cgaccactcc gacgcggcca gcgccggcga
      301 agctgccaac gggacggatc agcagcctct cggacacggt caggctgctg ccgggcgtcg
      361 gcgaggcgcg ggcgaagctg ctggagcagc ttggggtgcg gacggttcgc gacctgttgc
      421 ggctgtatcc acgccgtcac atcgactaca gtaatgtcca gaagatcggc agcctgctct
      481 tcggccacat ctcaacgatt cagggaacgg tccagtcgat cgagtcgtcg cggacgcgga
      541 ccggtaagca actcttcgat gtcgtcgtcg atgacggcac cggccggatt catgcggcct
      601 tcttcaatcc gtggatcgag cgacagttgc ggcctggtgt gccggtctcg ctctcgggcc
      661 ggatcgagca gatgcgcggg aacctctgcc tctccaatcc ggagtgggaa gtcctcgatc
      721 acgagacgat caacaccggc cggatgatcc cggtctaccc gctgacgaag gggctgtatc
      781 agaagacgct ccgggcgctg gtgcgctacg cgctggatgg cgccggccac ctgctcgaag
      841 actacatccc cgacgacctg cgccggcagg aaggcgtcat cgggctggtt gaggcgaccg
      901 agtggatcca ttttccggac ggcgagacgc cggtcgaggc ccagcgacgg ctggacgcgg
      961 cccggggccg gctggcgttc gatgagtttc tggtcctcca acttggcctg ttgcagcgca
     1021 agcaggactg gcaatcgcag cccggcaccc gcattgagat cgatcgggag gcgctggacc
     1081 gcttcggcgc gtcgctgccg ttcgcgatga ccggcgctca gcagcgggcg ttgcgcgaga
     1141 ttctgggtga catggccgcg ccgcgaccga tgacgcggct gctccagggc gatgtcggct
     1201 ccggtaagac gatcgtcgcc gcgctggcgg cactggctgc cgtccgggcc ggctaccagg
     1261 tcgcattgat ggcgccgacc gagctgctgg ccgagcagca ctcgcggggg ctacgaacgg
     1321 tcttcgacag cggccccgag gatctgcggc cccggctggc gatgctgacc ggcagcgtcg
     1381 gcggaaccga gcgggagatg atctacgagg cgactgccga tggcagactc gacctgatcg
     1441 tcggcaccca ggccctgatc cagagcgggc tggagtttcg ccggcttggg ctggcgatca
     1501 tcgacgagca gcaccgcttc ggcgtcgagc agcgcgccag cctgcgcggc aagggcgaga
     1561 gtccggacct gctggtgatg acggcgacgc cgattccgcg cacgctggcg ctgacgttgc
     1621 acggcgacct ggatgtctcg acgctcgacg agctgccgcc tggccgccag ccgatcgaga
     1681 cgtactgggc ctcggaggcc aagcgggctg gcgcgtacag tttcgttcgc gagcaggtcg
     1741 cgcaggggcg gcaggcgttt atcgtcttcc cgctggttga ggagtcggag gcgatcgacg
     1801 cccgcgcggc agttgccgag catgagcggc tggcggccga ggtcttcccc gatctgcgcc
     1861 tgggcctgct gcacggccgg atgaagccgg ccgagaaaga cgccgtcatg ctggcctttc
     1921 gcgaccgcga gatcgacatc ctcgtctcga cctccgtcgt cgaagtcggg atcgacgtcc
     1981 cgaacgcaac cgtcatgctg atcgatggcg cagagcggtt cggcctctcg cagttgcacc
     2041 agttccgcgg ccgggttggc cgcggcccag agaagtcgta ttgcatcctg ctctcgaccg
     2101 atcccagcat cgagagcaag cagcgtttgc aggcgatggt cgattcgcag gacggcttcc
     2161 ggctggcgca gatcgacctc gacctgcgcg ggccgggtga cttcctcggc aagcgccaga
     2221 gcggcctgcc ggagatggaa cttgcgtctt tcgccgacgt gcgcgatctc gaacgggcgc
     2281 ggatggcggc cgagcgggtg ctggccgaag atccgaagct tgactcagcc aagtacgcac
     2341 tgctcaaggc gcgcgtcgat gccttctggc agaccgccat cgcggacgtg agctagcgga
     2401 tgcgtgttgt cagcggcgag gcacgtggcc ggaagctgcg cgcgccgaag agccagggga
     2461 tcacccggcc gatggccgac aagatcaagg aggccctctt ctcagtgctg gcctcgcttg
     2521 gcgtcgagta cgaccgggtc ctggacctct acgccgggtc gggcggcgtc gggatcgagg
     2581 cactctcgcg cggggcgagc tggtgtgatt tcgtcgaccg cgacactcat gccgtccgcg
     2641 cgattcgcga gaatctggag catgtcggct tcgccgatcg ggcgcgcgtc catcaggtgc
     2701 cggtcctttc ggcgatccac ggcgcgcacg agccgtatga catggtcctg ttcgatccgc
     2761 catacgctga tccggacatc atttccacgc tgaacgcgct atcattctcc cacgcggtac
     2821 gggatggcac gattgtggcg gtggggcact caccgcgtgt tgacctgccc gagcggatcg
     2881 gcaggttgga gaagttgcgc gggcgctgtc atggcgactc gtgcttctcc gttttcgatg
     2941 tggtgctgga tgcgggggag gacgccgcag cgtccgtcca gccggcatcg tcgcatgagg
     3001 agtaaggcag gacacatgcg cgtcgcggtt tatcccggca cgtttgatcc gatcacgaac
     3061 ggccacatcg atctggcgaa gcgggcggcg cgcctcttcg accggctgat cgtggcggtc
     3121 tattccggcg gcgatcgcgc gccgaagcga ccgctgttca cgaccgagga gcgggtcgcg
     3181 atggcgcgcc agagcttgtc ggatatcccg catgtggaga ttgacacgtt tgagaatttg
     3241 accgtagact acgctcgaag tgtgggtgca caaaccatcg tccggggtct ccgggcagtc
     3301 tcagatttcg agtacgagtt caaactcgcc catatgtatc agcatctggc gccggagctg
     3361 gaggttgtct gcctgatgac cagttcggcc cactcgttca tctcctccag cttcatccgc
     3421 gaagtcgcgt cgctgggagg tgatatcgac gatctcgtcc ctgcccacgt cgcggaggca
     3481 ctcagcatgc gcttcgatgc ggggcagctg caatccgatg gtaacgggtt gctggtgcga
     3541 acccagggcg gcatgtagtg ggcgatagcc cgtattcagc aagacggaac gagcacgaca
     3601 tgggctatga gacgcagacc cacggcaggg atatgatgga cttactcgac cggctcgaat
     3661 cgcaggtggc ggacggccgt cgcgtgttgc tctcaaaccg gatcatggtc gaggcggagg
     3721 agttcatggc gctcctcgac cagttgcgtg attgcattcc ggccgagatc cagcaggcac
     3781 gccgggtgct gcaggagcgc cagaagatca tcgttgaagc tcagacggaa gcaaccaaaa
     3841 tcatcacaat ggcaaaagag cgctcggaat acctcgtcag ccagcatggc gtgacagctg
     3901 aagcgcgcta ccgcggcgag gactcgctgc gcgaggcccg cgagaaggcc aaccgctcga
     3961 tgagcgccat cgacaagtac gcccgcgaac gcctggaaac cgtcgaaaag gtgatccgcg
     4021 acaacctggc cgagatcgag cgcgcgaagg ggagtctgtc gcaggtgtag cgttcgggaa
     4081 tcgggacacg ggattcggga ggtggtgagc ggcgtatccc gcccccgaat cccgtgtccc
     4141 aatttccgaa cgtttgacca ccccatcggc tcacgctata cttcacaggt tgtgcttgag
     4201 cgcagccatg gtgtgccgcc gccggggtca cagagtcgca ccagagtcac gagcaaccgg
     4261 cgagagtaga cattccaatg gatctcaaga acgacaccgt catcaacgtg gcgcagttga
     4321 tgaaggagga catcggagcc gcgcgcaagg tacacatgtc actggactgg ttcgcgctgg
     4381 accaggatct gatggcaaag gacctgagcg ccgatctgcg gctcacgcgc attacgaacg
     4441 gcatcctggc gagcgggcat gtcgcgggta tcgcgatggt tgaatgcgtg cgctgcctgg
     4501 agctgtacga tcagccgttc tcggccgatt tcgatcagga gtaccgcccg acgatcgacg
     4561 ttcggagcgg tgtcccgctc gatcagccgg cggctgacga agagcttggg acgatcgacg
     4621 cgtcgcacga gctggatctg gcggagccta tccggcaggt ggcgatcctc gatctgccga
     4681 tcaagccggt ctgccacgac gattgtgccg gggtgggcca gtccgagttc ggagacgagg
     4741 atgagggcga tcgtcggctg ggcgtcctga gtcagttgct tgagggcgac gacgagccgt
     4801 cgtaaccaat tgatagagtt agattgccgc gcgggcgcgg atgacatggg aactgggtag
     4861 ggaagaggac agatgggcgc acttccaaag caacgcacca ccagggcgaa gcagggcttc
     4921 cgccgccagc acgacaagct gacgccggtc cacctgatga cctgcccgca gtgccgccag
     4981 tacaagcggc agcaccacgt ctgcccgagc tgcggctact acgccggccg gcaggtgctg
     5041 gagatcgagg tcaagcgccg ccgcgagagc tgatccgaac cggtctcagg cgcgcggcgg
     5101 ggtgccgcgc gcctgttttt tgggctatcc ttgcgacagc ctgaaatgac acgactcgat
     5161 cgtctcgcag gtggcatatg ggagggacat cgctgatgcg gcacgccgcg gtcacgggtt
     5221 ggggcatggc tgtccctgac cgggtgttga ccaaccacga cctgtcgcag atggtcgata
     5281 cttccgacga gtggatcgtc agccggaccg gaattcgcga gcgccggatc gtcagtgccg
     5341 gcgatacggc gacgagcatg tccgtcacgg ccggccgggc tgcctgcgag aaagccgggg
     5401 tcgatccggc tcaattagac ctcatcattg tcggcaccta caccgcggat caccagatgc
     5461 cctcggtcgc cagcctcgtc cagaacgaac ttggcgcgcg ccgggccggc gcgttcgacc
     5521 tcaatgcagc ctgctccggc ttcgtatacg cgctggcggt cggttcgcag ttcatccaga
     5581 cgggcgtcta ctcgaaggtg ctcgtgatcg gcaccgacct cgcctcgcgc ttcctcgatt
     5641 acacggaccg tggcacctgt atcctgtttg gcgacggctc cggcgccgtt gtgctccagc
     5701 cgagcgaggc gaatgagggc gtcctctcga tcgcgctcgg ttcggatggt tcggccggcc
     5761 accacctgac gcttggcgac aacacgggct tgacgaacct caacggttcc cggacgaccg
     5821 aacggccctt catgaagatg aacgggccgg aggtgttccg ctttgccgtc aaggtgatgg
     5881 gcgaggtcgc ggccgaggcg gtcatcaagg ccgggctgac ttttggcgac gttgacctgc
     5941 tggtgccgca tcaggcgaat ttgcgcatca ttgcgacggc cgccaagcgg ttggaattgc
     6001 cgcgcgagcg ggtctgggtc aacgtcgatc ggtacgggaa tacgtcgtca gcgtcggtgc
     6061 cgatctcgct ggtggaagcg gtcgacgcgg gcgcgattca ggatgggatg aacgtcgtcg
     6121 tcgttgggtt tggcggggga ctgacatggg cagcgggggt actgcgatgg ggctcgtcgg
     6181 gcgtcgcgca gggggaccgt cggtgacagc cggcgcggca tggatctttc cagggcaggg
     6241 atcgcaatcg gtcggcatgg gccgcgatct cgccgcgcgc tatcccgaag cgcagtcagc
     6301 atttgacgag gccgacgcag cgcttggttt ctcggtttcc agtctctgct gggacggtcc
     6361 cgataccgaa ctgacgcgca ccgccaacca gcaacccgcc ctcctgaccg tcagcatcgc
     6421 gaccctgcgg gtgctgctca aggaagacct cctgccggaa ccggattacg tggccggtca
     6481 ctcgctcggc gaatactcgg cgctggtggc cgccggcgcg ctctcgttcg ccgatgccct
     6541 gcggctcgtc cgccggcgcg gcgaactgat ggagcagtac gggcgtggcg gtatggtcgc
     6601 cgtcatcggg ctcgacgagg aggcgatgca actggtcgcc aacgagagcg gagccgaggt
     6661 cgccaacttc aacgcgccgg gccagacgac gctctccggc tccgacgagg cactcgaggt
     6721 cgctgagatc gccgccaaga cgcgcggcgc gcgacgcgtc atcaagctgc cggtcaacgg
     6781 ggcgttccac tcgtcgctga tgatgccggt cgcggacgag ctctaccgcg acatcgtgca
     6841 gaccgagatc gtcaagccgc gtgtgccact ggtctcgaac gtcaccggcg agccgatcgc
     6901 caacccggac gacattcgca acgaactggt cgagcagatc tgcgcctcgg tccgctggac
     6961 acgcggcatc gagacgatga ttgagctggg tgtaacccac ttctacgaga tcggcgccgg
     7021 caatgtcctg accgggctgg ttggccggat cgacaagtcg gcgacggcgc tggcgtccga
     7081 ccggctgctg ggcaatcgcc tcaagaacgg gggtggcgcg tgagcgagac gagcgaacgg
     7141 cgtgccgcga ttgtcacggg cgcgacccga gggattggcc gggcgatcgc ccttcaactc
     7201 gcaaccgagg gcttcaacat cgtcgtcaac taccggggcg aagaggcgtc ggccacgtcg
     7261 ctggtcgatg agcttcaggc gcacggcgtt gaagctcgcg cgatccgcgc cgacatcacg
     7321 agcgccgacg atgtcgccgg cctgattgag ggaacgatt
//
LOCUS       DASUGJ010000205         5084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1002084, whole genome shotgun sequence.
ACCESSION   DASUGJ010000205 DASUGJ010000000
VERSION     DASUGJ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002084"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFV93_04515"
     CDS             <1..383
                     /locus_tag="VFV93_04515"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEX5164638.1"
                     /translation="LAVLLNAVHGTPGATANRNDLVAIFLTGIPGLNMVADSPRRAVK
                     SDMLRINLAIPNSSFPNGRTLTDDVVDIELSVVGLSDGTFEGLKTSGISDGVNANDLP
                     FQDGFPFVPVPHAGFSFDPDNGAD"
     gene            464..1834
                     /locus_tag="VFV93_04520"
     CDS             464..1834
                     /locus_tag="VFV93_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5164639.1"
                     /translation="MSQTITSQRPPRQHRTPRLYRLLLVPLAILLVIGAFLAGNAIRS
                     GSTALKSDAPPATNRAAATTADQIATLQARLTTNADDIDSATTLAMLYIQQARETGDP
                     RWYGKAETLLNGVLDRQPEHVEALAGQGALALARHDFARGLEWGLKARSIDPEALAVY
                     PVLIDAYVELGRYDEAATAANAFVRMKPNLASYTRVAYLRELFGDLDGAAEALQLALD
                     TVPAWTEPAGWTRVQLANLYLNSGDLDTAEREYQQTLALLPNYAPATAGLGKVAAARG
                     DTARAIELLSDAATRLPLPEYVIALGDLYTASGDRQAAASQYALVQVMTQLQTANGVN
                     TDLELALFAADHPEFANGLTADGIVEQARVALSRRPSIYGHDTLAWALYRAGDYDAAW
                     TQSQQALQLGTRDALLHFHAGMIASARGDTAAAKSELETALAINPHFSILHAATARET
                     LAKLNR"
     gene            1898..3457
                     /locus_tag="VFV93_04525"
     CDS             1898..3457
                     /locus_tag="VFV93_04525"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164640.1"
                     /translation="MTAHLTTRRLGTIGIIAALALTLLSMLLPASADAHPLGNFTINR
                     YARLEFSEDAVTIVYALDFAEIPTFQQMELLDTDDDGNVSDAESERYLNEIVPMLVDG
                     LRVVVGDEVLPLAIVDRRLELLPGEGGLKILRLDARFTSTLPDGWQDDGYGGYTDRNY
                     QDRLGWREVVVQGGPGVAIESSTAPQEDVSNELRAYPDNALTSPLDVAEATFTIVPGD
                     GTPIGRSATAADAAERVERSTNGGRATSRVANLITREQTVPVFTVSLLLAFFWGAVHA
                     LSPGHGKTVVAAYLVGARGTARHAAFLGLTVTITHTAGVFALGMVTLYLSRVILPEDL
                     YPWLSVASGLLVVAIGVALIWSRLRARSDHLHDHSHPHDHQHVHDHAHDHDHPDHYDG
                     ALVHSHGGQTHTHLPPGADGSRVTWRSLLALGVSGGLIPCPSALVLLLGAISLDRTEL
                     GMVLVVAFSFGLAAVLTAIGLLLVYARRYFERTSFTPRIPRLLPVASAFAVSIAGLAI
                     VYQSLHQAGVM"
     gene            3693..>5084
                     /locus_tag="VFV93_04530"
     CDS             3693..>5084
                     /locus_tag="VFV93_04530"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEX5164641.1"
                     /translation="MTSDQDDEHRPETPDDETTSSSPFRRLTPAGEPAWHSPPRPEPV
                     DAFVPGAATIAMPHERSSAPEPTPAPATGSSRARYTAVFGLVGGVLLLMAVAAGAYAA
                     YQALLGHDDRATAAYAPANAWAYVAVNVDPTSHAWLDAWTLAKRAGIDDELSQLPKDG
                     LEESGEDPGVWESLIQPALGRELGFAVWPNAAGIDEEPEVAAIVMIADEDKARDALDT
                     LLPGDPRTETIYRDVTYETNDDGDAVGIIDEALILASSSAAFEAVVDAHADSALNGVD
                     EFTSAADRAADSPLVFVYVDGASIADAAASLQGELPAGPDMMSLMTPDFGDAFDVYAE
                     LGRITLTVKADDDALQTEVLTEGRPASFPMTPAGNAFADQMPASTLFYVASADLYGTI
                     FAPAIGQYEQMLGTAGADGTFGMPSPDDLTAMLGFDLEGGLLEQFDGPYALSVNVEES
                     DGQYGGQFHFFSDV"
BASE COUNT          828 a         1842 c         1641 g          773 t
ORIGIN      
        1 acctggccgt tctgctcaac gccgtccacg gcacgccggg ggcgacggcg aaccgcaacg
       61 acctggtcgc gatcttcctg accggcatcc ccggtctgaa catggtcgcc gacagcccgc
      121 ggcgggccgt caagtccgac atgttgcgca tcaacctggc gatcccgaac tcgtccttcc
      181 cgaatggtcg gaccctgaca gacgacgtcg tcgacatcga gctgtcggtt gtcggcctca
      241 gcgacggcac gttcgaggga ctcaagacca gcggcatcag cgatggtgtc aacgccaacg
      301 atctcccgtt ccaggacggt ttcccgttcg tgccggtccc tcacgccggc ttcagcttcg
      361 acccggacaa cggcgccgac tagcgccgcg atccaccaga cacgggagcc ggtgcgagcc
      421 ggctcccaca accctcacca acgaccgaag ggacggcgcg tcgatgagcc agaccatcac
      481 ttcgcagcgg ccaccacgac agcaccggac gcccaggctc taccggctgc tgctcgtgcc
      541 gctggcgatc ctgctcgtca tcggcgcttt cctggccggg aacgcgatcc gttcgggaag
      601 cactgcgctg aagagcgacg cgccaccggc cacgaaccgc gccgccgcga cgaccgccga
      661 ccagatcgcc acgttacagg cgcggttaac gaccaatgcc gacgacatcg actcggcgac
      721 gacgctggca atgctctaca tccagcaggc gcgcgagacg ggcgacccgc gctggtacgg
      781 caaggctgag acgctgctga acggcgtcct cgaccgccag ccggaacacg tcgaggcgct
      841 ggccggacag ggcgcgctgg cgctggcgcg acatgacttc gcgagggggc tcgagtgggg
      901 cttgaaggca cgctccatcg atccggaggc gctggccgtc tacccggttc tgatcgacgc
      961 ctacgtcgag ctgggccgct acgacgaggc ggccacggcg gccaacgcgt tcgtgcggat
     1021 gaagccgaac ctggcctcct acacgcgcgt cgcctacctg cgcgagctgt tcggcgatct
     1081 cgacggcgcc gccgaggcgc tccaactggc actcgacacg gttcctgcct ggaccgagcc
     1141 ggccggctgg acgcgggtcc aacttgccaa cctgtatctc aacagcggtg acctcgacac
     1201 cgccgagcgc gagtaccagc agacgctggc actgctgccc aactacgccc cggcgacggc
     1261 cggtctcggc aaggtcgcgg cggcacgggg tgacacggca cgggcgatcg agctgctgag
     1321 cgatgccgcg acgcggctgc cgctaccgga gtacgtcatc gcgctcggcg acctctacac
     1381 cgccagcggc gaccggcagg ccgccgccag ccagtacgcg ctggtgcagg tgatgacgca
     1441 actccagacc gccaacggtg tcaacaccga cctggaactg gcactcttcg cggccgacca
     1501 tccggagttc gccaacggcc tgacggcgga tggcatcgtc gagcaggcgc gggtcgcgct
     1561 cagccggcgt cccagcatct acggccacga cacgctcgcc tgggcgctct atcgggccgg
     1621 cgactacgac gccgcctgga cccagagcca gcaggcactc cagctcggca cccgcgatgc
     1681 gctgctgcac ttccacgccg gcatgatcgc cagcgcccgc ggcgacacgg cagcagcgaa
     1741 gtcggaactc gaaaccgcgc tcgccatcaa cccgcacttc tcgatcctgc acgccgcgac
     1801 cgcccgcgag acgctggcga agttgaaccg gtgatccgcg aggatcacga caccgtagaa
     1861 ttgctgaccg gtggaacgac cagggatcat ggggtcaatg actgcacatc tcaccacacg
     1921 caggctgggg acgatcggca tcatcgcggc gctggcgctg acgctgctct cgatgctgct
     1981 accggcgtcg gcggacgcgc acccgctcgg gaacttcacc atcaaccgct acgcgcggct
     2041 ggagttctca gaggacgccg ttaccatcgt ctacgcgctc gacttcgccg agatcccgac
     2101 cttccagcag atggagctgc tcgacaccga cgatgacgga aatgtctccg acgcggaatc
     2161 cgagcgctac ctgaacgaga tcgtcccgat gctggtggat ggcctgcggg tcgtcgtcgg
     2221 cgacgaggtc ctgccgctcg cgatcgtgga tcggcggctg gagctgctgc cgggcgaggg
     2281 cgggctcaag atcctgcggc tcgacgcgcg cttcacatcg acgctgccgg atggctggca
     2341 ggacgacggc tacggcggct acaccgaccg caactatcag gaccggctcg gctggcgcga
     2401 agtcgtcgtc cagggcggtc ccggcgtcgc gatcgagagc tcgaccgccc cgcaggagga
     2461 tgtctccaac gaactgcgcg cctaccccga caacgccctg accagcccgc tggatgtcgc
     2521 cgaggcgacc ttcacgatcg tgccgggtga tggcacaccg atcggccggt ccgcgacggc
     2581 cgcagacgcc gccgagcgcg tcgagcggtc caccaacggc ggtcgggcaa ccagccgcgt
     2641 cgccaacctg atcacccgcg agcagaccgt ccccgtcttc accgtctcgc tgctgctggc
     2701 cttcttctgg ggcgccgtcc acgccctctc gccggggcac ggcaagacgg tcgtcgccgc
     2761 ctacctggtt ggtgcgcggg ggacggccag gcacgccgcc ttcctcggcc tgacggtgac
     2821 gatcacgcat acggccggcg tcttcgcgct ggggatggtg acgctctacc tctcgcgcgt
     2881 catcctgccg gaggacctct acccgtggct cagcgtcgcc tccggcctgc tggtcgtcgc
     2941 gatcggcgtc gcgctgatct ggagccgcct gcgcgcgcgt tccgaccatc tccacgatca
     3001 ttcgcaccca cacgaccacc aacacgttca cgatcacgcc catgaccacg atcatccaga
     3061 ccactacgac ggcgcgctcg tccactccca cggcgggcag acccacactc atctgccgcc
     3121 gggagccgat ggctcgcgcg tcacctggcg cagcctgctg gcgctcggcg tcagcggagg
     3181 gctgattccg tgcccttcgg ccctcgttct gctgctcggc gcgatctcgc tcgaccgaac
     3241 cgagctggga atggtgctgg tcgtcgcctt ctcgttcggg ctggccgctg tcctgaccgc
     3301 gatcggcctg ctgctcgtct acgcccgccg ctacttcgaa cgcacctcgt tcaccccacg
     3361 catcccacgc ctgctccccg tcgccagcgc cttcgccgtc tcaatcgccg gcctcgcgat
     3421 cgtctaccag tcgctgcacc aggcgggagt aatgtaacgg ggtcgggggg cgggattcgg
     3481 gacttggggt tcgtgggcat cgagcaggac acgcctctca aatcaatccc gcgtcccgaa
     3541 tcccgagtcc cggccccgtc tacaaacagg acccttttcc cgtctccaac ggccatcctc
     3601 gctgctagtc tgaagcccaa cgacagcctt cacaggttgg cgtgggcggt tgtgcgtccg
     3661 cgccggccgg cagatagaca aggggcttct cgatgacaag cgaccaggat gacgagcaca
     3721 ggcccgagac gcctgatgac gagacgacat cctccagtcc atttcgccgt ctgacgccgg
     3781 ccggtgagcc ggcctggcac agcccgcccc ggccggagcc ggtcgacgcg ttcgtgccgg
     3841 gcgcggcgac gatcgcgatg ccgcatgaga ggtccagcgc gccagaacct acgccggctc
     3901 ccgccaccgg tagttcgcgg gcacgctaca cagccgtctt cgggctggtc ggcggtgtcc
     3961 tgctgctgat ggccgttgcg gccggagcgt acgccgccta ccaggcgctg ctcggccatg
     4021 acgaccgtgc gactgccgcc tatgccccgg ccaacgcctg ggcgtatgtg gccgtcaatg
     4081 tcgatccgac cagccacgcc tggctggacg cctggacgct ggcgaagcgg gccgggatcg
     4141 acgatgaact ctcgcagttg ccgaaggacg ggctggagga gtccggtgag gaccccggcg
     4201 tctgggagtc gctgatccag ccggcgctcg ggcgcgagct gggcttcgcc gtctggccca
     4261 acgccgccgg gatcgacgag gaacctgagg tcgccgcgat cgtgatgatc gccgatgagg
     4321 ataaggctcg tgacgcgctc gacacactgc tgcccggcga tccgcggacc gagacgatct
     4381 atcgcgatgt gacctacgag acgaatgacg atggtgacgc cgtcggcatc atcgatgagg
     4441 cgctgatcct ggcctcgtcg agcgcggcct tcgaggcggt cgtcgacgct cacgcggata
     4501 gcgcgctcaa cggcgtcgac gagttcacat cggcggcaga ccgcgcggcc gatagcccgc
     4561 tcgtcttcgt ctacgtcgat ggcgcctcga tcgccgacgc ggccgcgagc ctccagggcg
     4621 agctgccggc cggcccggac atgatgtcgc tgatgacgcc ggacttcggc gatgccttcg
     4681 atgtctacgc cgagctgggc cggatcacac tgacggtcaa ggccgacgac gacgccctcc
     4741 agaccgaggt gctgaccgaa ggccgcccgg cgagcttccc gatgacaccg gccggcaacg
     4801 cattcgccga ccagatgccg gcctcgacgc tcttctacgt cgccagcgcc gacctgtacg
     4861 gcacgatctt cgcgccggcg atcggccagt acgagcagat gctcggcacg gccggtgctg
     4921 acggcacgtt tggcatgccc tcccccgacg acctgacggc gatgctcggc ttcgacctcg
     4981 aaggcggcct gctggagcag ttcgatgggc cgtatgcgct gtcggtcaac gtcgaagagt
     5041 ccgacggcca gtacggcggc cagttccact tcttcagcga cgtg
//
LOCUS       DASUGJ010000206         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1974347, whole genome shotgun sequence.
ACCESSION   DASUGJ010000206 DASUGJ010000000
VERSION     DASUGJ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974347"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFV93_04535"
     CDS             <1..226
                     /locus_tag="VFV93_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164642.1"
                     /translation="LLGARVEHGPESMIYEQVSQVTRSLLPIIEQTGIATAEDVDIET
                     LADRLRHEATALDATLVSPEFIGAWTTTPA"
     gene            275..1645
                     /locus_tag="VFV93_04540"
     CDS             275..1645
                     /locus_tag="VFV93_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5164643.1"
                     /translation="MTHHHHAGSAIVIGGSIAGLLAARVLSEHFAQVIILERDDLTGG
                     TEPRKGIPHAQHAHALLAGGLLGIEGLFPGISQRLVALGAVRGQGRFFSGGGYHARRA
                     GGPGGLFVSRPLLEAEIRARVRGLPNVAIVEHVDARGLAADASQTQVTGVCVVRRDGA
                     GDEEVIPADLVIDCSGRGSRASDWLAALGFPAPEVERVDVGMGYSTRLYRREPEHLTG
                     DVMINIAPSIDNRRACGMLAQEGDRWIVTLAGYFGDHPPTDAQGYVSFARTLPVPDVY
                     DVIHAAEPLTDPVAYRFKANQWRHYEWLDRFPQGFLVLGDAIASFTPVYGQGMTVAAL
                     EATALARCLDDTKDDLAARFFAEARAIIAVAWSITVGNDLRLSPQATRPAAMRAIGWY
                     TSRLQLAARYDPVLADAFMQVANFLAPPPSLLRPGIALRVLRGNLHPPQLRRQATTEV
                     PEPARM"
     gene            complement(1681..>2279)
                     /locus_tag="VFV93_04545"
     CDS             complement(1681..>2279)
                     /locus_tag="VFV93_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164644.1"
                     /translation="GRSAAGKTTLADELAPAIRARGRDVLRASIDDFHRPGHKYRSIR
                     DEWTPQTRYDEGCDYDAFVELVLRPLGPGGSRRCRPALFDSYHDVWLPEKWVDVDADT
                     VAIIDGGFLLRPELASHWDYVIWLDIDIETMVDRARRRDVAWTGSQERVVERYLNLWI
                     PTHLLYERLIEPMTKADLVIDNRDVAAPVIHHLSCPPP"
BASE COUNT          367 a          784 c          761 g          367 t
ORIGIN      
        1 gctgctcggc gcccgcgtcg aacacggccc ggagtcaatg atctacgagc aggtgtcgca
       61 agtgacccgc tcgctgctgc cgatcatcga gcaaacgggc atcgccacgg ccgaggatgt
      121 cgatatcgag acgctggccg accgccttcg gcacgaggca acggcgctgg atgccacgct
      181 cgtgtccccc gagttcatcg gcgcctggac gacgacgccg gcctgaccca ccgctgcgta
      241 cacccctggc aacctgaacc gaacggagca gaccatgacg caccaccacc acgccggaag
      301 cgccatcgtc atcggcggca gcatcgccgg attactggcc gcccgggtgc tttcggagca
      361 ttttgcccag gtgatcatcc tcgaacgcga cgatctgacg ggcgggaccg agcctcgcaa
      421 gggcatcccg cacgcccagc acgcccatgc gctgctggcg ggcgggctgc tgggcatcga
      481 aggactcttt cccggcatct cccagcgcct cgtcgcgctt ggggctgtcc gtgggcaggg
      541 ccgattcttc tccggcggcg gttaccatgc gcggcgcgcc ggtggtccgg gcggtctgtt
      601 cgtgagccgg ccattgctgg aggccgagat ccgcgcgcgt gtgcgcggcc tgcccaacgt
      661 cgcgatcgtc gagcacgttg acgcgcgcgg gctggcggca gacgcgagcc agacgcaggt
      721 caccggcgtt tgcgtcgtcc gacgagatgg tgcaggggat gaagaagtga ttccggccga
      781 tctggtcatc gactgcagcg gccggggatc gcgggcgtcc gactggctgg cggcgctcgg
      841 attcccggcg ccggaggtag agcgggtcga cgtcgggatg ggctactcga cgcgcctcta
      901 ccgacgcgag ccggaacatc tgaccggcga cgtgatgatc aacatcgccc cttcgatcga
      961 caaccggcgc gcctgcggca tgctggccca ggagggcgac cgctggatcg tcacgctggc
     1021 cggctacttc ggcgaccatc cgccgaccga cgcgcagggc tacgtgagct tcgcgcgaac
     1081 gctgccggtg ccggatgtct acgacgttat ccacgccgcc gagccgctga cggacccggt
     1141 tgcctaccgc ttcaaggcca accagtggcg ccattacgag tggctggacc gtttcccgca
     1201 aggcttcctc gtcctcggcg acgcaatcgc cagcttcaca ccggtctacg gacagggcat
     1261 gaccgtcgcg gcactggaag caaccgccct ggcccgctgc cttgatgaca cgaaggacga
     1321 cctcgcggca cggtttttcg ccgaggctcg cgcgatcatc gccgttgcct ggagcatcac
     1381 ggtcggcaac gacctgcgct tgtcgccgca ggccacccga ccagcggcca tgcgcgcgat
     1441 cggctggtac acgtcccggc tccagctggc cgcccggtat gaccccgttc tggcagacgc
     1501 cttcatgcag gtagcgaact tcctggcccc acctccgtcg ttgctgcggc cggggatcgc
     1561 gctgcgggtc ctgcgcggca acctccaccc accgcagctt cgccggcaag cgacgacgga
     1621 ggtgccggaa ccggcacgga tgtaatctgc ctgcgtcgtt ccccatcagt ccggtcaaga
     1681 tcacggtggc ggacaactga ggtggtggat cactggggcg gccacatcgc ggttgtcgat
     1741 caccagatcg gctttcgtca tcggttcgat cagccgctcg tagagcaggt gggtcgggat
     1801 ccagaggttg aggtagcgct cgacgacccg ctcttgggaa ccggtccagg ccacgtcgcg
     1861 tcgccgcgcg cgatcgacca tcgtctcgat gtcgatgtcg agccagatca cgtagtccca
     1921 atggctcgcc agctcggggc gcaacagaaa gccgccgtcg atgatggcga ccgtgtcggc
     1981 gtcaacgtca acccatttct cgggaagcca gacatcgtgg tacgagtcga acagcgccgg
     2041 gcggcagcgg cggctaccgc ccggacccag cgggcgcagg acgagctcga cgaacgcgtc
     2101 gtagtcgcag ccctcgtcgt agcgcgtttg cggcgtccac tcgtcacgga tcgatcgata
     2161 cttgtgaccg ggtcggtgga agtcatcgat cgaggcgcgc aagacatccc gcccacgtgc
     2221 gcggatcgcc ggcgcaagct catcggcgag cgtcgtcttg cccgctgctg agcggccgt
//
LOCUS       DASUGJ010000207         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_723589, whole genome shotgun sequence.
ACCESSION   DASUGJ010000207 DASUGJ010000000
VERSION     DASUGJ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723589"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..826
                     /locus_tag="VFV93_04550"
     CDS             86..826
                     /locus_tag="VFV93_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEX5164645.1"
                     /translation="MVDVQEPVVQEQVKKPASKSMPKWESEARDRLRAGIRRFSKPLT
                     DLIARDANEGDTRLLVTDFLCEALGYDKYTDLTTEYQVKGEFADYGVRIDQQIRAFIE
                     VKRVTTKLNARHLRQVEMYAVNEGVEWVILTNGSQWQAYHLTGGLPVAIDLAFEIDLL
                     GDATTTQKVNELFYLTREAFRKNLIDELWKAKRATSPVSLARILCSENVATSIRKELR
                     RETGRNVDEVEIVKLLRDTVIRPEALKA"
     gene            848..1201
                     /locus_tag="VFV93_04555"
     CDS             848..1201
                     /locus_tag="VFV93_04555"
                     /inference="COORDINATES: protein motif:HMM:NF018644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEX5164646.1"
                     /translation="MRRARFSPYWYYALVGGAVGLALTAIGVAVLDLPLYPAWIIGLS
                     ICTFVLYGFDKRRAISGAGRVPEAVLHGLALAGGFPGGWAGRSAFRHKTRHTSFLVVL
                     VLATVLHVALYLWLR"
     gene            complement(1212..1403)
                     /locus_tag="VFV93_04560"
     CDS             complement(1212..1403)
                     /locus_tag="VFV93_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164647.1"
                     /translation="MTLAHLVRHEQNERGEILGIGPQEPLMETKQRSGWMTVMIALSL
                     LVAAVFVLYLIMTLAGILD"
     gene            1664..2143
                     /locus_tag="VFV93_04565"
     CDS             1664..2143
                     /locus_tag="VFV93_04565"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5164648.1"
                     /translation="MTTADPVVSLRDATRKMLQWQHRNLRHVVTSLSAEALNWTPLPE
                     ANSIASLVFHTLGAESELIAAAAGMLIEHDGTESFGFVATSAEDLLAMIDHTEHDING
                     YLDQLRGDHLGRILTRRGWTESGAWWLLHAVGHTREHVGQAQLTRQWWERTHASADG"
     gene            2327..2590
                     /locus_tag="VFV93_04570"
     CDS             2327..2590
                     /locus_tag="VFV93_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEX5164649.1"
                     /translation="MHCLSMGGKHAEAGHIRLLLDCAEICATSANFMLRESDLHAQTC
                     AVCAEVCERCADDCERIGPDDVMMTRCAEMCRRCADSCHRMAA"
     gene            2816..>3888
                     /locus_tag="VFV93_04575"
     CDS             2816..>3888
                     /locus_tag="VFV93_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164650.1"
                     /translation="MSRRAAGWLAWGLLAVFGVSIGAGLVLQYARDEPPGAFVTYLSL
                     AVAFGLFAVMGLLIVTRRPDNVMGWLYCAIGLGTAYTFVSPSYRLYTLERPGRDFPLV
                     AITSLLESVVWSLNLTLIIVFALLLFPTGRPLSRRWSIVGWIAGIGLTLSTLATVLTG
                     GQILGIGETVDNPIGIEAFRPVGMLLYDVADILMLVSCPLAVAAIIVRFHRARGVERQ
                     QIKWLAFAATGGFVLAAGNALLQSPLGDVGFAIAIAMIPLMTGLAILRYRLFDIDRLN
                     NRALVYSLLSGVLALVYLAAIAVFDVTLRGMSGQSSDLATAASTLLVAGLFRPVRGRI
                     QSTVDQRFYRRAYDAARVLEQFG"
BASE COUNT          684 a         1192 c         1219 g          793 t
ORIGIN      
        1 gatggtgatg ccatgcgaga ccggtcagac gagtcgtctg accgattcgt gacgtggcgc
       61 gcatcgcgca atgggggaag ccgtcatggt tgatgttcaa gagccagtcg tccaggagca
      121 ggtcaagaag cccgcgtcta agagcatgcc gaagtgggaa tcagaggcgc gggatcggct
      181 tcgagcggga atcaggcgtt tcagcaagcc gctgacagac ctcattgctc gtgatgccaa
      241 cgagggtgac accaggctgc tcgtgaccga ctttctttgt gaggcccttg gctacgacaa
      301 atacactgat ctgacaacag agtatcaagt caaaggcgag ttcgcggact atggagttcg
      361 cattgaccag caaatccgag cgtttatcga agtcaagcgt gtcacaacca aactgaacgc
      421 tcgccatctt cgacaggtcg agatgtatgc cgtcaatgag ggtgtcgagt gggtcatcct
      481 gacgaatggt agccagtggc aggcctatca tctgacaggc gggttgccgg tcgctattga
      541 tctggccttc gagatcgatc tgctgggcga tgccacgaca actcagaagg tcaacgaact
      601 cttctacctg acgcgtgaag ccttccggaa gaacctgatc gatgagctct ggaaagccaa
      661 gcgagccacg tcgcccgtct cgctggcccg aatcctctgc tctgagaacg tcgcgacgtc
      721 cattcggaaa gaactccgcc gcgagactgg ccgcaatgtc gatgaagtag agatcgtcaa
      781 gctgcttcga gacaccgtca tccggcccga agccctcaag gcgtagaagt acagcagatg
      841 tgatttgatg cgccgcgcca gattctcacc gtattggtac tacgcgctgg tcggtggcgc
      901 agttggcctc gcgttgacgg cgattggtgt cgccgtcctc gatctgccgc tctatccggc
      961 ctggattatc gggctgagca tctgtacgtt cgtgctctac gggtttgaca agcgccgggc
     1021 gatcagcggg gcgggccggg ttcccgaagc cgtcctgcat ggcctcgcgc tggcgggagg
     1081 attccctggt ggctgggccg gacgctcggc gttccgccac aagacacgcc acacgagctt
     1141 tcttgtcgtg ctcgtgctcg cgaccgtgct ccacgtcgcg ctctacttgt ggttgcgctg
     1201 atggttgccg gctaatccag aatcccggcc agcgtcataa tcaggtagag gacgaagacg
     1261 gctgcaacca gcagcgacag ggcgatcatc accgtcatcc agcccgaacg ttgtttcgtc
     1321 tccattagcg gctcctgtgg tccgattccg agaatctctc ctcgctcgtt ctgctcgtga
     1381 cgcaccaggt gtgccagtgt catttcgccg agcgtcgttg gcagcgcgtt tacgggttcg
     1441 gacgacgaac gaccatcatg ggtagatgtg atgagctgtg ttccgtgttc taacgtccga
     1501 gcatgacatg cggatagcac ctgtctcaaa tcacaccgct ggctccggct actgagacac
     1561 cagtgggcga cgatcgctga cgacttgcaa ctcgaccctc agtgtcatag gctgcgacgc
     1621 ttcgagactg gcggcgatct gtgtatccaa aggagcccag aacgtgacga ctgctgatcc
     1681 agtcgtgtcc cttcgggacg cgacgcgcaa gatgttgcag tggcagcacc gcaacctccg
     1741 acacgtcgtc acctcgctga gcgccgaggc cctgaactgg accccgctgc ccgaggcgaa
     1801 ctccattgcc tcgctcgtgt ttcacacgct cggcgctgaa agcgaactca tcgccgcggc
     1861 cgccggcatg ctcatcgagc acgacggcac ggagagcttc gggtttgtcg ccacgagcgc
     1921 tgaggacctc ctggcgatga tcgaccacac ggagcacgac atcaatgggt accttgacca
     1981 actgcgcggg gaccacctcg gcagaatcct gacgcggaga gggtggacag agtctggcgc
     2041 atggtggctg ctgcacgcgg tcggacatac ccgcgaacac gtcggtcagg cccagttgac
     2101 gcgccaatgg tgggaacgca cacacgcatc agccgacggc tgatcaggcg gaccccacac
     2161 gctgcaggtg ctgagctcgg cacgtgttgt gctgctcgat tccagcgggc caatcatcca
     2221 tcgccagtga gagtatcgaa agggagagcc tgtgtcgcat tccgatctga tgcgtgagtg
     2281 catcaacaac tgcaccaact gtcacactat ctgtactgag acggtgatgc actgtctgag
     2341 tatgggcggc aagcatgccg aggcgggcca tattcggctg ctgctcgatt gcgccgagat
     2401 ctgcgcgacg agcgcgaact tcatgctgcg cgaatccgac ctgcacgcac agacctgcgc
     2461 cgtctgtgcc gaggtctgcg aacgctgtgc ggacgattgc gagcggatcg ggccggacga
     2521 cgtgatgatg acccgctgcg ccgagatgtg ccgccgctgc gcggattcct gccaccggat
     2581 ggctgcctag gctcgatcgt ccgtcaccct caccacaaca cgcatcccgc cagaccccgc
     2641 cgcccaaccg ccgcactcac ttgcgtcgtg caggatgatc tcgtaacgat ttcggaacgg
     2701 tctgaggcgt acagtgtgac cgagcgaagg ctcgatgtcg cgggcagtcg gtcgtcgacc
     2761 tcgcgaggtg tgtcgtgtgc gtgtctgcgg cgccagcctg gaaggtgggg ttacgatgag
     2821 tcgtcgagct gctgggtggc tggcctgggg ccttcttgcg gtgttcggtg tctccattgg
     2881 agccgggctg gtcttgcagt acgcgcgtga cgagccgccg ggtgcgttcg tcacctatct
     2941 ctcgctcgcg gtcgccttcg gcctgttcgc ggtgatgggt ctgctgatcg tcacccgccg
     3001 gccggacaac gtcatgggct ggctctattg cgcgatcggg ctcggcaccg cctacacgtt
     3061 tgtcagcccc agctatcgcc tctatacgct ggaacgacct gggcgagact tcccgctggt
     3121 cgcgatcaca tcgctcctcg aaagtgtcgt ctggtcgctg aacctgacgc tgatcatcgt
     3181 cttcgcgttg ctgctgttcc caaccggccg gccgttgtca cgacgctggt cgatcgttgg
     3241 ctggatcgcc gggattgggc tgacgctgtc gactctcgcg acggtgttga ccggcggcca
     3301 gattctggga atcggcgaga cggttgataa cccgatcggg atcgaagcgt tccggccggt
     3361 gggcatgctg ctgtacgatg tcgccgacat cctgatgctg gtttcgtgcc cgctggccgt
     3421 cgccgcgatc atcgtgcggt ttcaccgtgc gcggggcgtc gagcgacagc agatcaagtg
     3481 gctcgcgttc gcggcgaccg gcggcttcgt gctggccgcc ggcaacgcgc tcctgcaatc
     3541 tcccctcggc gatgtggggt ttgccatcgc gatcgcgatg attccactca tgacagggct
     3601 ggcgatcctg cgctaccggc tcttcgatat cgaccgtctc aacaatcgag cgctggtcta
     3661 cagtctgctg tccggtgtcc tcgccctggt ctacctggcc gcgatcgccg tcttcgacgt
     3721 gacgttgcgt ggcatgtcag gccagtcgtc cgatctggca acggccgcct cgacgctgct
     3781 cgtcgccggc ctgttccggc ccgtccgtgg gcgcatccag tcgacggttg accaacgctt
     3841 ctatcgtcgc gcctacgacg cggcccgggt gctcgaacag ttcggtca
//
LOCUS       DASUGJ010000208         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_863559, whole genome shotgun sequence.
ACCESSION   DASUGJ010000208 DASUGJ010000000
VERSION     DASUGJ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863559"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFV93_04580"
     CDS             <1..466
                     /locus_tag="VFV93_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164651.1"
                     /translation="QLDVFQVIPGCHGPHEVTYDQRSNLDPEGIVELFIWNQAEWDAD
                     MTTPIPGLESWTATQLLEHECANSTRIDVPGGYAIATATWRGNTLLDWINAGTTGCPA
                     EPYDYFLARVFRDGLVVTVFQDSPDNWDGDTPFESVDNLLTIVNALQYADVE"
     gene            533..841
                     /locus_tag="VFV93_04585"
     CDS             533..841
                     /locus_tag="VFV93_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164652.1"
                     /translation="MIAALVLFKTNGALSLDEAASRFNSTAPKYRGRDGLHSKAYLYA
                     EDGNALGGFYIFDSREDAEALYSDAWRSRAVELYGVEPTIQYFEIPVLIENAVASLSS
                     "
     gene            1009..1488
                     /locus_tag="VFV93_04590"
     CDS             1009..1488
                     /locus_tag="VFV93_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010174760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5164653.1"
                     /translation="MDQQSVDTRPAVTTVEELKGYIGQEISVSAWQTITQERVDQFAD
                     ATGDHQYIHVDPERAKTTFFGGTIAHGYMTLSMTHPAGEGIKVDLGGKLAVNYGLNRV
                     RFPAPVPVGSRVRQRVVLSNVEEIGNQAVQLTHTRTVEIEGGDKPACVAETLMRIYF"
     gene            1535..2587
                     /locus_tag="VFV93_04595"
     CDS             1535..2587
                     /locus_tag="VFV93_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5164654.1"
                     /translation="MANTNERVEDLTDQADTQDSGPGTQHSDDRYKVFHNKGRWQGLP
                     LRVEDTGEAPHFEPLNPRIPRPTPRVPFPDILAFGVPNHGMMAGQPTNVYLMGYVADE
                     GRSLALVDTGDKSSEDVLLAALAQTGVAIERITQIILTHCHPDHVGNAAALKQATGAT
                     VYAHPLERQQIERFGNDLEVDVWVEPGQSIDCDGFTVETIFTPGHSPGHYCVVEPGSG
                     VLLAGDMISGFGSVGIFPPHGSMRQYIDSLRRLLAAHEARPFSLVGPGHGPAIEDARA
                     KIEEYITHRLAREEEVYDAVVSGASSVDDILPIAYPDVQPHLSFAARSTLQAHLDKLI
                     EDGRIRRVADDRYAVA"
     gene            2684..3646
                     /locus_tag="VFV93_04600"
     CDS             2684..3646
                     /locus_tag="VFV93_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX5164655.1"
                     /translation="MSTNPPLVVAPNGSETFLNAPLVTELAALDAEVAILGVPHGVPY
                     NMRGVAGGASDAPAAIRHQSLRFGHGRFIGHYDFDFDDGDTLGGRPLRIVDCGDVTAD
                     PLDIPGNAARATEAVRSIIARGVLPVILGGDDSIPIPFFRAFDGHGPLTLIQIDAHLD
                     YRDEIDGVHEGFSSPIRRASEMPWIERIVQLGMRGVGSARQRDVNDARANGNLIVRAP
                     ELREAGVASVLDRIPAGPYLITIDADGLDPSVAPGVGAPAPGGLTFDETTSLLRGLAG
                     KGRIAGLDVVEIVPALDRNNITSLVAFQLIMTTLGAAVRAGQFG"
     gene            complement(3743..4042)
                     /locus_tag="VFV93_04605"
     CDS             complement(3743..4042)
                     /locus_tag="VFV93_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX5164656.1"
                     /translation="MLTVITETTVKSGHEADWDRAYQERSTDARSQPGWVDLHLLVPV
                     DDARRRLVVGTWQDRESWERWHTTETFQRTRDQLNDATETHGDDRWYEVVEQRTA"
BASE COUNT          767 a         1448 c         1305 g          733 t
ORIGIN      
        1 acaactagac gtgttccagg tcattcccgg ctgccacggc cctcacgaag tgacgtacga
       61 ccagcgcagc aacctcgatc ctgaaggcat cgttgagttg ttcatctgga atcaggccga
      121 atgggatgca gacatgacga caccgatacc tggtctcgaa tcttggacgg cgacgcaact
      181 gctggagcac gagtgcgcga attcgacgcg aattgacgta ccgggcggct acgcgattgc
      241 gactgcgacg tggagaggta acacgcttct cgattggatc aatgctggca ccaccggctg
      301 tccagcggag ccgtacgact actttctcgc aagggttttt cgcgacggcc ttgtcgttac
      361 cgttttccag gattcgcctg acaactggga cggtgacacc ccgttcgagt ccgtcgacaa
      421 cttactgacg atcgtgaacg cgcttcagta tgcggacgtg gaatagacgc tacccggatg
      481 ctgctatcct gtttggaaga tgacgcagca ctgaccggga aggacgtcat taatgatcgc
      541 cgcactcgtg ctcttcaaga ccaacggcgc gctgtcactc gatgaggcag ccagccgctt
      601 caattcgacc gcccccaagt atcgtgggcg cgacggactc cactcgaagg cgtatctcta
      661 cgccgaggat ggcaacgcgc tgggtggctt ctatatcttc gactcccgcg aggacgccga
      721 ggcgctctac tctgacgcct ggcgatcccg tgccgttgaa ctgtacggcg tcgagccgac
      781 catccagtac ttcgagattc cggtgctgat cgagaacgca gtcgcgtccc tgtcgtcctg
      841 agccgccgcg aaggacctct ccgcgttagc cgagctatgg cgtcaacgca ggggaggtcc
      901 ttcgctgccg ctaaggacga cgggggctgc tatgcgatca ccacacgacg gggtacgcta
      961 gcagcggcat catgtcgtgc gtaccgcatc tcgaatcagg agccgcagat ggaccagcaa
     1021 tccgttgaca cccgcccggc cgtcacgacc gtcgaggagc tgaagggcta catcggccag
     1081 gagatcagcg tcagcgcctg gcagacgatc acgcaggagc gcgtcgatca gttcgcggac
     1141 gccaccggcg accatcagta catccacgtc gatccggagc gtgccaaaac cacgttcttc
     1201 ggcgggacga tcgcgcacgg ctacatgacg ctctcgatga cacatccagc cggtgaagga
     1261 atcaaggtcg atctcggcgg gaagctggcc gtcaactacg ggctgaaccg ggtacgcttt
     1321 ccggcgccgg tcccggtcgg ctcgcgcgtc cgccagcgcg tcgtcctgag caacgtcgag
     1381 gagatcggca accaggccgt ccagctgacg cacacgcgga cggtcgagat cgagggcggt
     1441 gacaagcccg cctgcgtcgc cgagacgctg atgcggatct acttttgagt gctgagtctc
     1501 cggtgctgag tgctgagttg aaagaaacgg attgatggcc aacaccaacg aacgcgtcga
     1561 ggaccttacc gaccaggcag acactcagga ctcgggccca ggcactcagc actccgacga
     1621 ccggtacaag gtttttcaca acaagggccg ctggcagggg ctaccgctcc gcgtcgaaga
     1681 caccggcgaa gcaccacact tcgaaccgct caatccgcgc atcccgcgtc ccacaccccg
     1741 cgtcccattc cccgacatcc tcgccttcgg cgttcccaac cacggcatga tggccggtca
     1801 gccaaccaac gtctatctca tgggatatgt cgcggacgag ggccggtcgc tggcgctggt
     1861 cgacaccggc gacaagagtt ccgaagacgt cctgctggcc gcgctggccc agaccggcgt
     1921 tgcgatcgag cggatcacgc agatcatcct gacccactgc cacccggatc acgtcggcaa
     1981 cgcggccgcc ctcaagcagg cgaccggcgc gacggtctac gcccacccgc tggaacggca
     2041 gcagatcgag cgtttcggca acgacctgga ggtcgatgtc tgggtcgagc cgggccagtc
     2101 gatcgactgc gacggcttca cggtcgagac gattttcacg cccggccact cgcccggcca
     2161 ctactgcgtt gtcgagccag gcagcggtgt tctgctggcc ggcgacatga tctccggctt
     2221 cggcagcgtc ggcatcttcc cgccgcacgg cagcatgcgg cagtacatcg actccctgcg
     2281 ccgcctgctg gctgcccacg aggcgcggcc gttctcgctg gtcggccccg gtcacggccc
     2341 ggcgatcgag gatgcccgcg ccaagatcga ggagtacatc acccaccggc tagcccgcga
     2401 ggaggaggtc tacgacgccg tcgtgtccgg agcgagcagc gtcgacgaca tcctgccgat
     2461 cgcctacccc gacgtccagc cgcacctctc gttcgcggcc cgctcgacgc tccaggccca
     2521 cctcgacaag ctgatcgagg atggtcgaat ccgtagggtc gctgacgatc gctacgcagt
     2581 cgcctgaggg ccgatacgat ggtgaccaac gaccatcgcg gagtggggcg gctgaggtcc
     2641 gtgcgggcgg ttacgtcggt ggcagggaaa ggatgtgaac caggtgtcga ccaatccacc
     2701 gctggtagtc gctccgaatg gctcagagac gttcctgaac gcgccactcg tgacggagtt
     2761 ggccgcgctc gacgctgagg tcgcaatcct cggcgtcccg catggggttc cctacaacat
     2821 gcgcggggta gcaggcgggg cgagcgacgc gccagccgca atccgtcacc agtcactccg
     2881 cttcggtcac ggtcgcttca tcggtcacta cgacttcgac ttcgacgacg gcgacacgct
     2941 cggcggccgg ccactcagga tcgtcgactg cggcgacgtg acggctgacc cgctcgacat
     3001 cccgggcaac gctgcccgcg ctaccgaggc cgtccgctcg atcatcgcgc gcggagtact
     3061 gcctgtcatc ctcggcggag acgactcgat cccgatcccg tttttccgcg ccttcgacgg
     3121 acacggtcca ctgacgttga tccagatcga cgcgcacctc gactaccgtg acgagatcga
     3181 cggcgtccac gagggtttct cctcaccgat ccgacgggct tcagagatgc cgtggatcga
     3241 gcggatcgtc cagcttggga tgcgcggcgt cgggagcgcc cgccagcgtg acgtgaatga
     3301 cgcgcgggcc aacggcaatc tcatcgtccg tgcccccgag ctgcgcgagg cgggcgttgc
     3361 ctccgtcctc gaccggatcc cggccggccc ctacctgatc acgatcgacg ccgacgggct
     3421 cgacccgtcc gtcgcgccgg gcgtcggcgc acccgccccc ggcggtctga cctttgatga
     3481 gacgacgagc ctgctgcgcg gcctggccgg caagggccgg atcgccggcc tcgacgtcgt
     3541 cgagatcgtc ccggctcttg accggaacaa catcaccagc ctcgtcgcct tccagttgat
     3601 catgaccacc ctcggcgcgg ccgtccgcgc cgggcagttt gggtaacagg agcgccaccc
     3661 cgtgcaattc acgggcgtga tctctaccga cacgtcgtca cgcgaatgac gtgcacccgg
     3721 catcgccacc gtcgccgacg cctcacgccg tgcgctgctc gaccacctcg taccaacgat
     3781 cgtcgccgtg tgtctcagtc gcgtcgttga gctggtcacg tgtgcgctgg aacgtttcgg
     3841 tcgtgtgcca gcgttcccaa gattcgcgat cctgccaggt accaacgacc agccggcgtc
     3901 gcgcgtcatc aactggaacg agcaggtgga gatcgaccca gccgggctgg cttcgcgcgt
     3961 cggtcgaacg ctcctggtac gcacggtccc agtccgcctc gtgacccgac ttgaccgtcg
     4021 tctccgtaat caccgttaac atcgccccca cctttcgcat tgaacagcgg tccagcaccc
     4081 gtccccggcc gacgctgcat ctgccgtgcc gcggcgatcg cacggatcgc tgatgcatca
     4141 tcagtggtgg cgaggacgcc cagatcgtcg gatcgttcgc cagtactcgc tactcggtac
     4201 tcgccgctgc ccatcctgtt agaatattca acggttgagt attcgagcga ggg
//
LOCUS       DASUGJ010000209         5757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1974798, whole genome shotgun sequence.
ACCESSION   DASUGJ010000209 DASUGJ010000000
VERSION     DASUGJ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974798"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /gene="pdxH"
                     /locus_tag="VFV93_04610"
     CDS             complement(<1..593)
                     /gene="pdxH"
                     /locus_tag="VFV93_04610"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX5164657.1"
                     /translation="MSESKTEHHGLAIADLRREYSLEGLSEADLDPNPFGQFERWFRQ
                     AMDAHVLETNAMTLATATRDGIPSARVVLLKGMDDEGFVFFTNYESRKGAELTENPRA
                     ALLFYWAELTRQIRIEGDVSRVSIEESAAYFASRSELSQLGAWASHQSMQIPDRAYLD
                     NRLAELIEQYRDQQIPLPPYWGGFRLVPRAFEFWHGRP"
     gene            862..1992
                     /locus_tag="VFV93_04615"
     CDS             862..1992
                     /locus_tag="VFV93_04615"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEX5164658.1"
                     /translation="MTHAWGRGLRWLLAVGVMSGLLAACGGNGEAEPTATTAPVATAT
                     TAAAATTTDLSAMKGYSLDRAAEMKTATAALAEDAASFYADAEAADFDYQAMFDADPE
                     GIAALLAKTKQDWIAASTAYEQNEGIIAGVPSLSYYDVWIDAGPSGEEDATGALDWQL
                     ELPNGETLDKPGNFFHNLTEPAIWGTDDDFVGLRADLDQDGTQELGEVMPEANIFKAA
                     TEGLDAATAEMTGAINDWEPTPEDAFTALVVMIPTMNEYFEQWKLSVFVAGAQETEEV
                     AFVALSRLFDINGILAGLDLTYDQLSTNVTTADSGLDAQIQEGFETLIGHVGDLYQQE
                     ADGKHFTADEADVFGSEAQDQATQLAGQVSQAAALLGVAVAE"
     gene            1998..4406
                     /locus_tag="VFV93_04620"
     CDS             1998..4406
                     /locus_tag="VFV93_04620"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX5164659.1"
                     /translation="MRQGWMRKIARSSEWLLLVVLLAALVPAGLVSAQTTQQPNSPWE
                     TADRLRKELFAGQAALLTDDSTAATAAVERAESIWTGELQPIYVPDANRAADLVTAGF
                     AQAETAAGSSNAAGLVVARATIWSGLLLGAADMTVSAAENGDAEAAQRWLLLRAFVPA
                     TRISRPGAGATLAVQALADGRLQPADAAEAVRADLLHTYQALALSSLDEMLEAGNSGS
                     SLRQAESAALAAGYWRIVAESYGIQHGDESRQALDAQADSLAATALSDPAALPQLADS
                     MRQELRRFQASPMTDEAAARRANQLLRFLSLVPFEYRQGVKDGHVILALEIEEAITFR
                     DGAAAAFNDLASALYAIDAEQAASVDGLLRELDADLQGARSGSEVADSNDVDELTNTL
                     TSELKDLFPSAWSDNDSGTTDFDTISGLLDELEAAVASGKYGQAESLRLEAYAVFDVG
                     PELRLIGLAPGLAARIEGYFWHGHGGTAGLASLIAKRASPGDIQASRQTLDGSLAEAD
                     ARLGAGPPAHGAIVFNSAVIVFREGLEAVLIFASLLASLIGARRVYRRPMVLGAILAG
                     VATLITWVLFGLVLSSLRRYGERLEAVVSLIAVAVLLLILNWFLHDAYWSRWIQRFHG
                     RKRSLVGAAAGQTIGFVLMGFTSIYREGFETTLFLQALVLDAGTVAVLEGVALGLLGT
                     FAIGVLVFKVQAKLPYKRMLILTGLLITAVLMTMVGNTVHLFQAVGWMPINLIDGVQF
                     PYWAGVWLGLYPTWEGIIAQVLSGAFVISSYFIAEELQARERRKAHVAPLATPSATTS
                     GGHD"
     gene            complement(4377..>5757)
                     /locus_tag="VFV93_04625"
     CDS             complement(4377..>5757)
                     /locus_tag="VFV93_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX5164660.1"
                     /translation="AGDDFDAVMAEYGESLERFDRRGGYDLDYRIDIVFAGLRISHLP
                     RQRPFSGLSGGEKARVLLATLLLRSPDVLLLDEPTNHLDFASIAWLEDYLSCYRGGVL
                     VISHDRHFLNGSVTRIVEIDEHTRATKEYTGNYDAYLIERQRERAQWEVDYAEQQDEI
                     KELKRAIRVTAHNVAHNRAPRDPAKMAYDFKGGRVAGTISRNIRSAEERLRRIEADPI
                     PKPPSEMRIAPDFSPDELRSEQIIVAEGIRKQFGPQPVLDGVNVTLGPRDRVVIVGPN
                     GAGKSTLLDILAGRSPADAGTVSHATGARLGYLDQDGRSLDPAGTVLATYRDGLIGYE
                     DELIADLFRHGLFTLDDLDKRVGDLSAGQLRKLQLARLMAERGNVLLLDEPTNHLAFD
                     ILEEFEKALIDFPGPILAVSHDRWFIQRFVAGGGIVWELDEGRLQPALDPERLWRQAA
                     SVVPTAGGR"
BASE COUNT          971 a         1866 c         1955 g          965 t
ORIGIN      
        1 gggcggccgt gccagaactc gaaggcgcgc gggacgagtc ggaacccacc ccagtagggt
       61 gggagcggaa tctgctgatc gcggtactgc tcgatcaact cggcgagccg gttgtccaga
      121 taggcgcgat cgggaatctg catgctctgg tgcgaggccc aggcgccaag ctggctaagt
      181 tccgagcgac tggcgaagta ggcggctgac tcctcgatac tgacccggct gacgtcaccc
      241 tcgatcctga tctgacgagt cagttcggcc cagtagaaca gcaacgcggc gcgcgggttc
      301 tctgtgagtt ccgctccctt gcggctctcg tagttggtga agaagacgaa gccctcgtca
      361 tccatcccct tcagcaggac aaccctggcc gacggaatac cgtcgcgggt ggctgtcgcc
      421 agcgtcatcg cgttcgtctc caggacatgg gcgtccatcg cctgccggaa ccagcgctcg
      481 aactgtccga acgggttggg atcgaggtcg gcttcgctga gcccctcaag gctgtactcc
      541 cggcgcaggt cggcgatcgc caggccgtga tgttctgtct tgctctcact catcggtatc
      601 tacgtccact ccccaccagg catcgacgcg gacccacgcc ggctccgcag ccgggtatgt
      661 cgatgataca cgggacgatg cgcaacgcgt gatgcataac gcgtgatgcg gaacgggcga
      721 tagatccagc atgccccatt ccaccctacg cgtcacgcaa atcccctttg acaaacagca
      781 aggtcagaag tactctaata cccgactaat ttcatcggga atgaatgaga cggatcgctg
      841 actggcaggg agtggggaca gttgacgcac gcgtgggggc gcggactgcg atggctgctg
      901 gctgtcggag tgatgagcgg gctgctggca gcatgtggtg gtaacggcga ggccgaaccg
      961 acggcgacca ccgcgccggt ggcgacggcg acgacagcgg cggctgcgac aacgaccgac
     1021 ctgtcggcga tgaagggcta ttcgctcgac cgcgctgccg agatgaagac cgccacagcc
     1081 gccttggccg aggacgcggc gtcgttctat gcggacgccg aagcggctga tttcgactac
     1141 caggcgatgt tcgacgccga tcctgagggc atcgcggcgc tgctggcgaa gaccaagcag
     1201 gactggatcg ccgccagcac tgcctacgag cagaacgaag gcatcatcgc cggcgttccg
     1261 tcgctctcgt actatgatgt ctggatcgac gccggcccct ccggcgagga ggacgcgacc
     1321 ggcgcgctcg actggcagct tgagctgccg aacggcgaga cgctcgacaa gcccggcaac
     1381 ttcttccaca acctgaccga accggccatc tggggtaccg acgacgactt cgtcggcctg
     1441 cgtgccgatc tcgatcagga cgggacgcag gaactgggcg aggtgatgcc ggaagccaac
     1501 atcttcaagg ccgcgaccga gggcctcgac gccgcgaccg ccgagatgac cggcgcgatc
     1561 aacgactggg agccgacgcc ggaggacgcc ttcacggcgc tggtcgtgat gattccaacc
     1621 atgaacgagt acttcgagca gtggaagctg tcggtcttcg tggctggtgc gcaggagacc
     1681 gaagaggtcg ccttcgttgc gctgagccgc ctgttcgaca tcaacggcat cctggccggg
     1741 ctggacctga cctatgacca gctcagtacg aacgtgacga cggccgacag cgggctggac
     1801 gcccagattc aggaggggtt tgagacgctg atcggacacg tcggggatct ctaccagcag
     1861 gaagctgatg ggaagcactt cacggccgac gaggcggatg tcttcggcag tgaggcgcag
     1921 gatcaggcca cccaactggc tggtcaagtc agccaggcgg cggcgctgct cggcgtcgcc
     1981 gtggcggagt agtgatgatg cggcagggct ggatgcggaa aatcgcccgc tcgtcggaat
     2041 ggctgctgct ggtcgtcctc ctggcggcgc tggttccggc cgggctggtc tcggcgcaga
     2101 cgacacagca accgaactcg ccgtgggaga ccgccgaccg gctgcgcaag gagttgttcg
     2161 ccggccaggc tgccctgctg acggacgact cgaccgctgc gacggctgcc gttgagcggg
     2221 ccgagtcgat ctggacaggc gagctccaac cgatctacgt gcccgatgcg aaccgggcag
     2281 cggacctcgt caccgcaggt ttcgcgcagg cggagacggc tgccggctct tcgaacgcgg
     2341 ccgggctggt cgttgcccgg gcgacgatct ggagcgggtt gctgctcggt gcggccgata
     2401 tgacagtttc ggcggcagaa aatggcgacg ccgaggcggc ccagcgctgg ctgctgttgc
     2461 gcgccttcgt tccagcaaca cgcatctccc ggcccggcgc gggggcgacg ttagcggttc
     2521 aggcgctggc cgacgggcgg ctccagccgg ccgacgcagc cgaggcggtt cgcgccgatc
     2581 tgctgcatac ctatcaggcg ctggcgctgt cctcgctgga cgaaatgctg gaggccggca
     2641 acagcggctc cagcctgcga caggctgagt cggctgcgct ggccgccggt tactggcgaa
     2701 tcgtcgccga gagctacggt attcagcacg gcgacgagag ccggcaggcg ctcgacgcgc
     2761 aggccgacag cctggcagcg acggcgctga gtgacccggc tgcgctgccc cagctagcgg
     2821 actcgatgcg gcaggaactg cggcgcttcc aggcgtcgcc gatgaccgac gaggctgccg
     2881 cgcgacgcgc gaaccagctg ctgcgcttcc tctcgctggt gccgttcgag taccggcagg
     2941 gcgtcaagga cggccacgtc atcctcgctc tcgaaatcga agaagcgatc accttccgcg
     3001 atggtgctgc cgccgccttc aacgacctgg cgtcggcgct ctacgccatc gacgccgagc
     3061 aggcggccag cgtcgatggg ctgctgcgcg agctggatgc cgacctgcaa ggcgcgcgca
     3121 gcggcagcga ggtcgccgac tccaacgatg tcgacgaact gacgaacacg ctgaccagtg
     3181 aactgaagga cctgttcccg tctgcctgga gtgacaacga cagcggcacg accgacttcg
     3241 acacgatcag cggcctgctc gacgaactgg aagcagcagt cgcatccggc aagtacgggc
     3301 aggcggagtc gctgcggctc gaagcctatg ccgtcttcga cgtcggcccg gaactgcggc
     3361 tgatcggcct cgcgcccggg ctggcagcgc ggatcgaggg ctacttctgg cacggccacg
     3421 gcgggaccgc cggcctggcc tcgctgattg ccaaacgggc ctcgccgggc gacatccagg
     3481 catcgcgtca gacgctcgac ggctcgttgg ccgaggccga cgcgcggctg ggagccggtc
     3541 caccagccca cggcgcgatc gtcttcaact cggccgtgat cgtcttccgc gaggggctgg
     3601 aagccgtcct gatcttcgcc tcgctgctgg ccagcctgat cggcgcgcgt cgcgtctacc
     3661 gccggccgat ggtgctgggc gcgattctgg ccggcgtcgc gacgctgatt acctgggtgc
     3721 tgttcgggct ggtcctctcg tcgttgcgcc gctacggcga gcggcttgag gcggtcgtct
     3781 cactgatcgc cgttgccgtc ctgctgctga tcctcaactg gttcctgcac gacgcctact
     3841 ggtcgcgctg gattcagcgt ttccacggtc gcaagcgcag cctggtcggc gctgccgccg
     3901 gtcagacgat cggcttcgtc ctgatgggct tcaccagcat ctaccgcgaa gggttcgaga
     3961 cgacgctctt cctccaggca ctcgtgcttg acgccgggac ggtcgccgtc ctcgaaggcg
     4021 tcgcgctcgg gctgctgggg acgtttgcga tcggtgtcct cgtcttcaag gtccaggcca
     4081 agctgcccta caagcggatg ctgatcctga ccgggctatt gatcacggcc gtcctgatga
     4141 cgatggtagg caacacggtt cacctcttcc aggccgtcgg ctggatgccg atcaacctga
     4201 tcgacggcgt ccagttcccc tactgggccg gcgtctggct cggcctctac ccgacctggg
     4261 aggggatcat cgcccaggtt ctgtcgggtg ccttcgtgat cagtagctac ttcatcgccg
     4321 aggagctcca ggcgcgcgag cggcgcaaag cccacgtcgc gccactggcg acaccgtcag
     4381 cgaccaccag cggtgggcac gactgacgcc gcctgccgcc acaaccgttc gggatcgagg
     4441 gccggctgca accggccctc gtccagttcc cagacgatcc cgccgccggc cacgaaacgc
     4501 tggataaacc agcggtcgtg agagacggcc agaattggac cgggaaagtc gatcagcgcc
     4561 ttctcaaact cctccaggat gtcgaacgcc aggtggttgg tcggctcgtc gagcagcagg
     4621 acgttgccgc gctcggccat cagccgcgcc agttgcagct tgcgcagctg accggccgag
     4681 aggtcgccga cccgcttatc gagatcgtcg agcgtgaaca gcccgtgccg gaacaggtcg
     4741 gcaatcagct cgtcctcgta cccgatcaac ccgtcgcggt aggtggcgag aaccgtcccg
     4801 gcgggatcga ggctgcggcc atcctgatcc agatagccga gccgcgcgcc ggtggcatga
     4861 gagaccgtcc cggcgtcggc cggcgagcgg ccggcaagga tgtcgagaag cgtcgatttc
     4921 ccggccccgt ttggcccgac gataacgacc cggtcacgtg ggccgagtgt aacgttgacg
     4981 ccatccagga ccggctgcgg cccaaactgc ttgcgaatgc cctcggcgac gatgatctgc
     5041 tcgctgcgca gttcgtcggg gctgaaatcg ggcgcgatcc gcatctcgga gggcggcttc
     5101 gggatcgggt cggcctcgat ccggcgcaag cgttcctcgg ccgagcggat gttgcgggaa
     5161 atggtacctg cgacgcgtcc gcccttgaag tcgtacgcca tcttggcagg atcacgcggc
     5221 gctcggttgt gagcaacgtt gtgggccgtg acgcggatcg cccgcttgag ctccttgatc
     5281 tcgtcctgct gctcggcgta gtcaacctcc cactgcgcgc gctcgcgttg ccgctcgatg
     5341 aggtaggcgt cgtagttgcc ggtgtactcc ttcgtcgccc gtgtatgttc gtcgatctcc
     5401 acgatccggg tgacgctgcc attcaggaag tgccggtcgt gggagatgac aaggacaccg
     5461 ccgcggtagc aggagagata gtcctccagc caggcgatgc tggcgaagtc gagatggttg
     5521 gtcggttcgt cgagcagcag cacgtctggc gagcgcaaca gcagtgttgc cagcaggaca
     5581 cgcgccttct cgccgccgga gagaccgctg aacggccgct ggcgggggag atggctgatc
     5641 cgcagtccgg caaagacgat gtcgatccgg tagtcgaggt cgtagccgcc acggcggtcg
     5701 aagcgttcca acgactcgcc gtactcggcc atgacggcat cgaagtcgtc gccggcc
//
LOCUS       DASUGJ010000210         5480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1002660, whole genome shotgun sequence.
ACCESSION   DASUGJ010000210 DASUGJ010000000
VERSION     DASUGJ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002660"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..2566)
                     /locus_tag="VFV93_04630"
     CDS             complement(233..2566)
                     /locus_tag="VFV93_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5164661.1"
                     /translation="MPSVAGRLWSRFPSDLPSRLRFALLAFAAILVATCVPFAIFNSA
                     PIWSRCLLIALYAVVLIRWTAWFRGGYPAPADLAVELLALLATTVVTADPSNAASFLY
                     AGVIIRAYFGSNRQLAWTLGLYNLTFLASVLIAPNTDHSLLAPNVLSQPPTAIVAAAV
                     IAWFSRTVKQHHRLLAEQDLHVALQNDLLAEVRAGQRRFQSLVEALPGAILTSTPSGE
                     VRYVSPQAGRIFGIAAEDLQTSWTALITRFVHPEDQARVFAATGTAIATGEPFSIAFR
                     ATMATGDLVWLQIDSVLVDDGSPDSPLWQSLLFDVTERHTLTEQLTHQAYHDTLTGLP
                     NRRRFHERVDETLKRATRDGIVPAVCFIDLDNFKTVNDTLGHLIGDRLIVEAAKRLRR
                     SVRDVDVVARLGGDEFALLLETPKGQADIIRVVERIVNDLSEPFPIDGQPIVTSASVG
                     IAIADSPDLSRTELIRRADVAMYAAKDDGKARYAIFDPQMNRRTRDRLDLELELQHAL
                     ARDELRFVYQPIVDLDTGTIRAVEALLRWQHPTRGLVSPDAFLSVLEDSGMIVPVGHQ
                     MLARACRQLSQWQQTIPGASDLSLSLNLSPRQIQDSSLVEAVSRALAETGVCPGHLQL
                     EISEQTMLADVEHATQAIAELTALGNRIAIDDFGTGNASLGHLRQLPVQMLKVDRSYI
                     EAYGQSEQTTTMLRAMVTLGKTLGMQVTAEGIETIEQTALRTLGCDFGQGFYFARPLR
                     DTEVESLLTEQIIYPLAEGHPLLSAIPAGAMPHRQAG"
     gene            2867..3298
                     /locus_tag="VFV93_04635"
     CDS             2867..3298
                     /locus_tag="VFV93_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164662.1"
                     /translation="MATDASSVLAAPQIAGTFVNPKGFARQVTATVAGGVVGSAIASA
                     SGPGQGDVPSFGRVAFVAATSSEVAIIKTKSGMLKMKLTDQVLARRARADINAIELKR
                     GALLSGLMIQFNDGGTWSFDVPKANQKSAVHFVEQLGGVVR"
     gene            complement(3400..4284)
                     /locus_tag="VFV93_04640"
     CDS             complement(3400..4284)
                     /locus_tag="VFV93_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5164663.1"
                     /translation="MIDRAANLPFPEVDGVRHRFVSVNGIRFHVAEAGEGEPLLMLHG
                     WPQHWYEWRHLMAALRDDYRVICPDLRGFGWSDVPATGYEKERLVDDILGILDRLGIE
                     RVRLIGHDWGGWLGFLLCLRRPEQVERFLALAILHPWLQFDLTLLRNSWRFGYQGFLT
                     SPLIGAWLVQRPGLIDQLIRLDSANGGQWRKRDLRWYSALLQDPARARASVLLYRTFL
                     LREMLPILRGRYRATRLHVPTHVLTGARDLTITPALLRGYAPYADAMTVEVVPDAGHF
                     LPEECPALVAERARAFFS"
     gene            complement(4281..4964)
                     /locus_tag="VFV93_04645"
     CDS             complement(4281..4964)
                     /locus_tag="VFV93_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164664.1"
                     /translation="MMVWSTAIRAAGATGLYAIVHSALAALPAKRNARRVAGARAADG
                     LYRFAFNGFAVVSFAATLRSIWSLPDQRLYRVRGVPALLMHAGQFACALALLQTNRQN
                     GLGQVTGIRHAREFVTGQPISPTPVVQHPLPEGECLDGWTGAFRLSSHPNNYLVLFLY
                     WLSPVMTVKWASVGAVTAVYMVLGSLHEDHRLLAAYGDRYRCYRTKVPHLAFHSIDHW
                     RAALGSGEA"
     gene            complement(5085..>5480)
                     /locus_tag="VFV93_04650"
     CDS             complement(5085..>5480)
                     /locus_tag="VFV93_04650"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX5164665.1"
                     /translation="ELYDIPGAARPDEDMPAPPRLLGMWDSVLLAYADRSRIIPTEYR
                     KLVIRVNGDTLPALLVDGYVAGIWRAVDGGIEATAFHPLSNDVWAQLGNEARALLALL
                     ADRDPQVYRRYHHWWGKLPAGETRLLSSD"
BASE COUNT          941 a         1740 c         1900 g          899 t
ORIGIN      
        1 cgcagcgaag gatcgcctag cgttcatgac aggaacgcgc tgatcggcag agggcgttcg
       61 acacgttcgc acaactcgtg tctccaacgc caggcgatcc ttcgctgcgg ctcaggatga
      121 caggggtggc gtgtgcgggc cgcaccaacg ccgcccgtct ggggcgcatc acgcgtcacg
      181 cgttagcggt ggcgcgcaac ccgacgtgat gcagcgtctg tcgtacggtg ggctagccgg
      241 cctggcggtg cggcatggct ccagccggga tggcggacag cagggggtgg ccttcggcga
      301 gcgggtagat gatttgctcg gtgaggagcg attccacttc ggtgtcgcgc aggggccgcg
      361 cgaagtagaa gccctggccg aaatcgcagc cgagcgtgcg cagggccgtc tgctcgatcg
      421 tctcgatccc ttcggcggtc acctgcatgc cgagcgtctt gccgagcgtc accatcgccc
      481 gcagcatggt cgtcgtctgc tcggactgtc cgtaggcttc gatgtacgag cggtcgacct
      541 tgagcatctg gaccggtagc tggcgcaggt gacccagcga ggcgttgccg gtgccgaagt
      601 cgtcgatcgc gatccggttg ccgagtgcgg tcagttccgc gatcgcctgg gtggcgtgct
      661 cgacatcggc cagcatcgtc tgctcggaga tctccagctg gaggtggcct gggcagacgc
      721 cggtctcggc caacgcccgc gagacggcct cgaccaggct ggagtcctgg atctggcgcg
      781 gcgagagatt caggctcagg ctaagatcgc tcgcgcctgg gatcgtctgt tgccactggc
      841 tgagttgccg gcaggcccgc gccagcatct ggtggccgac cgggacgatc atcccgctgt
      901 cctccaggac cgacaggaac gcgtccgggc tgacgaggcc gcgcgtcgga tgctgccagc
      961 gcaggagtgc ttcaaccgcc cggatcgtgc ctgtgtcgag gtcgacgatc ggctggtaga
     1021 cgaagcgcag ttcgtcgcgc gcgagcgcgt gctgcagctc cagttcgagg tccagccggt
     1081 cgcgggtgcg ccggttcatc tgcgggtcga agatcgcgta gcgcgccttg ccgtcgtcct
     1141 tggcggcgta catggcgaca tcggcccgcc gaatcagctc cgtgcggctg aggtcggggc
     1201 tgtcggcaat cgcgatcccg acgctcgcgc tggtcacgat cggctgaccg tcgatcggaa
     1261 acggctctga gagatcgttc acgatccgct cgacgacgcg gatgatgtct gcctgcccct
     1321 tcggtgtctc cagcagcagc gcgaactcgt cgccgccgag ccgggcgacg acatcgacgt
     1381 cacggacgct gcgccgcagc cgcttagctg cctcgacgat cagacggtcg ccgatcaggt
     1441 ggccgagcgt gtcgttgacg gtcttgaagt tgtccaggtc gatgaagcag acggctggaa
     1501 cgatgccgtc ccgcgtggcg cgcttcagcg tctcgtcgac gcgctcgtgg aagcgccgcc
     1561 ggttgggcag accggtcagc gtgtcgtgat acgcctggtg ggtcagctgc tcggtcagcg
     1621 tatggcgctc ggtcacgtcg aagagcaacg attgccacag cggcgagtcg gggctgccat
     1681 cgtcgaccag gacactgtcg atctggagcc agaccaggtc gccggtcgcc atcgtcgcgc
     1741 gaaaggcgat cgagaacggc tcaccggttg cgatcgccgt gccggtggca gcgaagacgc
     1801 gcgcctggtc ttccgggtgg acgaaacggg tgatcaaggc ggtccaactg gtctggagat
     1861 cctcggcagc gatgccgaag atcctgccgg cctgggggct gacgtagcgc acctcgccgg
     1921 acggcgtgct ggtcagaatc gcccccggca acgcctcgac cagcgactgg aagcggcgct
     1981 gcccggccct gacctcggcc agcaggtcgt tctggagggc gacgtgcagg tcttgttcgg
     2041 ccagcagccg gtggtgctgc ttcacggtcc ggctgaacca ggcgatgacg gccgccgcga
     2101 cgatcgcggt tggtggctgg ctgaggacgt tcggcgccag caacgagtgg tcggtgttcg
     2161 gcgcaatcag aacgctcgcc agaaacgtga ggttgtagag cccgagcgtc caggctagct
     2221 ggcggttgga gccgaagtag gcgcggatga tcaccccggc atacaggaac gaggctgcgt
     2281 tggacggatc ggcggtgacg acagttgttg ccaacagcgc cagcagctcg acggcgaggt
     2341 cagccggggc cggatagcca ccacgaaacc aggccgtcca gcggatcagg acgaccgcat
     2401 agagcgcgat cagcagacag cggctccaga tcggtgcgct gttgaagatg gcaaacggga
     2461 cgcaggtcgc gaccagaatg gcggcgaacg ccagcagcgc gaaccgtaac cggctcggca
     2521 gatcacttgg aaaccggctc cagaggcgtc cggcgacgga aggcatgcgt gcgaaacctc
     2581 gcgctggcgg cgaactcgtg tcacccgccg ctgggtgaca ccatattgag cgcggagccc
     2641 gcgccatcct gggagggact ttggttggag atttgttcgg gccggcgcaa ttgtgacgca
     2701 tttgtgacga tcggctgggt tcgcgcaccg ctttcgcctg tcggtggacg gggtgtattg
     2761 ccgtttacag cttgactgtg ccccagatga cagccaaccc ggccggcagg cggcatcctt
     2821 ggtgccagcg agatgacaac ccacgttgga tcacgaggag gacagtatgg caaccgatgc
     2881 atcgagcgtg ctcgccgcgc cacagatcgc cggaacgttc gtcaatccga aggggtttgc
     2941 ccgtcaggtg acggcgaccg tcgccggtgg tgttgtcggc agcgcgatcg cgtcggcatc
     3001 cggtccgggc cagggcgatg tccctagctt cggccgggtc gctttcgtcg ccgcgacctc
     3061 gtcggaggtc gcgatcatca agaccaagtc gggcatgctg aagatgaagc tgaccgatca
     3121 ggtgctggcg cgccgggcgc gggccgacat caacgcgatc gagctgaagc gtggtgcgct
     3181 gctctccggc ctgatgatcc agttcaatga cggcggcacc tggtcattcg acgttcccaa
     3241 ggccaaccag aagtcggccg ttcacttcgt cgagcagctt ggcggcgtcg ttcgctaatc
     3301 cccagcaatc cgatctctca tacaattctc caatgcaata cctcaacccg gtacgggtga
     3361 tatcgcccgt accgggttga ggattggttc acgtcgtgct cagctgaaga atgcccgggc
     3421 gcgctcggcg acgagggctg gacactcctc gggaaggaag tggccggcgt ccggcacgac
     3481 ctcgaccgtc atcgcgtccg cgtacggcgc gtagccgcgc aggagcgccg gcgtgatcgt
     3541 caggtcccgc gctccggtca ggacgtgggt tgggacgtgc agccgcgtcg cgcgatagcg
     3601 gccacgcaga atcggcagca tctcgcgcag caggaacgtg cggtagagca ggacgctggc
     3661 ccgtgcacgc gccgggtcct ggagcagggc gctgtaccag cgcagatcac gcttgcgcca
     3721 ctgccctccg ttggcgctgt ccaaccggat gagctggtca atgagccccg gccgctgcac
     3781 cagccaggcg ccaatcagcg ggctggtaag gaagccctga tagccgaagc gccagctgtt
     3841 gcgcagcagc gtcagatcga actgcagcca gggatgcagg attgccagcg ccagaaagcg
     3901 ctcgacctgc tccggccggc ggaggcagag caggaagccc agccagccgc cccagtcgtg
     3961 accgatcagc cgcacccgct cgatccccag cctgtcgagg ataccgagga tgtcatcgac
     4021 cagccgttcc ttctcgtacc ccgtcgccgg cacatcggac cagccgaaac cgcgcaggtc
     4081 tggacagatc acgcgatagt cgtctcgcag ggctgccatc aggtgccgcc actcgtacca
     4141 gtgctgtggc cagccgtgca gcatcagcag cggttcaccc tctcccgcct cagcaacgtg
     4201 aaaccggatg ccgttgacgc taacgaaacg atgccggaca ccatcgacct cgggaaatgg
     4261 gaggttcgct gcccggtcga tcatgcctcg ccgctcccta gcgcggcgcg ccagtgatcg
     4321 atcgagtgga acgccaggtg aggaaccttc gtgcgatagc agcgatagcg gtctccgtag
     4381 gcggccagca accggtggtc ctcgtggagc gagccaagca ccatgtagac ggccgtcacc
     4441 gcgccgaccg aggcccactt caccgtcatg accggcgaga gccagtagag aaacaggacg
     4501 aggtagttgt tcgggtggct ggagagcctg aacgctcccg tccagccgtc gagacactcc
     4561 ccctccggga gcggatgctg gacgacgggc gttgggctga ttggttgacc agtgacgaac
     4621 tcgcgggcat ggcgaatgcc ggtgacctgc cccagcccgt tctgacggtt ggtctgcaac
     4681 agggcgagcg cgcaggcgaa ttgaccggcg tgcatcagca gcgccgggac gccgcgcacc
     4741 cgatacaggc gctggtcagg cagcgaccag atcgagcgca aggtagccgc gaaactgacg
     4801 acggcgaacc cgttgaacgc gaagcggtag agtccgtcgg ccgcgcgcgc tcccgcaacg
     4861 cgtcgcgcgt tgcgcttggc cggcaacgcg gccagcgcgc tatgcacgat cgcgtacagg
     4921 cccgtagcgc cagccgctcg aatggccgtc gaccagacca tcatgtcatg ccctcccagt
     4981 gagccgtaac aaggtcttcg gttgggccgc gaacgaaagg aacccgctga ctggatctac
     5041 ggttacgacc cggagctccc cttgcaactc tgttgccagc cggatcagtc gctggagagg
     5101 agccgggttt cgccggcggg tagcttgccc caccagtggt ggtagcggcg gtagacctgc
     5161 gggtcgcggt cagccaggag tgcgagcagc gcccgtgcct cattcccgag ctgcgcccag
     5221 acgtcgttgg ataacgggtg gaaggcggtc gcttcgattc caccgtcgac ggcgcgccag
     5281 atgccggcga catagccatc gaccagcaat gccggcagtg tgtcgccgtt tacccggatg
     5341 acgagcttgc ggtactcggt cggaatgatg cggctgcggt cggcgtaggc cagcaggacg
     5401 ctgtcccaca tgcctagcag ccggggcggt gcgggcatgt cctcgtccgg tcgcgccgcg
     5461 ccgggaatgt catacagttc
//
LOCUS       DASUGJ010000211         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1140861, whole genome shotgun sequence.
ACCESSION   DASUGJ010000211 DASUGJ010000000
VERSION     DASUGJ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140861"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VFV93_04655"
     CDS             complement(<1..954)
                     /locus_tag="VFV93_04655"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5164666.1"
                     /translation="MTTPIRSTSLTGKIARTSARHPWKTIAGWLVVLAIALTTMVTIG
                     NHFTMNDEFRTDLESRVADDLITQRMNGGVETPAQERVIVSSADLTVDDPAFAAVVAN
                     VAATLSANEAVAGVQTYYDAGQDELVSLDRHRTVVVTTVAGDPDDVVKNAAPILETID
                     ELRTPAPGFEVNTLGQASVIDTVNETAKEGMAKGESIGIVVALLVMAVAFGALVAAGL
                     PIVLAIVSLLVTTGITMAISHLIGVNAAVIQMIGMIGLAVGIDYTLFMVSRYREEREH
                     GLATIDAITRAGETASKAVLFSGMTVVVSLLGMLIVPWNLMT"
     gene            complement(987..1253)
                     /locus_tag="VFV93_04660"
     CDS             complement(987..1253)
                     /locus_tag="VFV93_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164667.1"
                     /translation="MNDPRASTGGLSEPGDYEIRLRGHLEARWSDWFDGLSLRHECDG
                     TTVIHGPVADQSALYGLLRKVRDLGLPLISVTRVESDQPDVPAV"
     gene            complement(1449..>2426)
                     /locus_tag="VFV93_04665"
     CDS             complement(1449..>2426)
                     /locus_tag="VFV93_04665"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5164668.1"
                     /translation="RAEEQGEPGLHGTADFMVGLSELRRERNDLDAATDYLLRAQELA
                     RGPGATLNRSRWNVMMARIKESQGDLDGALAYLNEAERLRMPEFFPNVRPIAALRARV
                     RIRQGQIGDALGWARDQNLSTDDDLSYLREFEHITLARLLLARQSTTEALDVLHRILD
                     AAEAGGRMGSVIELLILQALAQQVTGNTPAALLPLERALTLAEPEGYARVFLDEGPPM
                     TALLATAAQQGIAPNAVRQLLPASDMTENMAPVRQDLIEPLSDREFDVLRLLATTLDG
                     PDIARELNISLNTMRTHTKNIYSKLGVNNRREAVIRAAELDLLSRSRKG"
BASE COUNT          372 a          754 c          854 g          446 t
ORIGIN      
        1 ggtcatcagg ttccagggca cgatcagcat gcccagcagt gagacgacga ccgtcatgcc
       61 ggagaagagg acggccttgc tggctgtctc accggcgcgg gtgatggcgt cgatcgtggc
      121 caagccgtgc tcgcgttcct cacggtagcg cgagaccatg aagagcgtgt agtcgatccc
      181 gaccgccagg ccgatcatgc cgatcatctg gatgacggcg gcgttgacgc cgatcaggtg
      241 cgagatggcc atcgtgatcc cggtcgtgac cagcagggag acaatcgcca gcacgatcgg
      301 cagtccggcc gcgacgagcg ccccgaaggc gaccgccatc accagcagcg caacgacgat
      361 gccgattgat tcgcccttgg ccatgccctc cttggcggtc tcattgacgg tgtcgatgac
      421 gcttgcctgg cccagcgtgt tgacttcgaa gccgggcgcc ggtgttcgca actcgtcaat
      481 cgtttcgagg atcggcgcgg cgttcttgac gacatcgtcg gggtcgccgg ctaccgtcgt
      541 gacgacgact gtccggtggc gatcgagtga gaccagttcg tcctggccgg cgtcgtagta
      601 ggtctggacg ccggcgacgg cctcgttggc gctgagggtg gcggcgacgt ttgcaacgac
      661 ggccgcgaag gccgggtcat cgacggtcag gtcggccgag gagacgatca cgcgctcctg
      721 ggcaggggtt tcgacgccgc cgttcatgcg ctgggtgatg aggtcgtcgg ccacccgcga
      781 ttcgaggtcg gtgcggaact catcgttcat cgtgaagtgg ttgccgatcg tgaccatcgt
      841 cgtcaacgcg atcgcgagga cgacgagcca gccagcgatc gtcttccagg ggtgacgggc
      901 cgaggtgcga gcgatcttac cggtcaggga agtgcttcga atcggagtcg tcatctcagt
      961 gtcctttcgt cgcgtatctc tcgcggctaa acggccggga catccggctg gtcggattcg
     1021 acgcgggtaa cggagatcag gggcaacccc aggtcgcgga ccttgcgcag caggccatac
     1081 agggcggact ggtctgcgac cggaccatgg atgacggtcg tgccgtcgca ctcgtgacgc
     1141 aggctcagcc cgtcgaacca gtcgctccag cgcgcttcca gatgccccct gaggcgaatc
     1201 tcgtaatcgc ccggctcgga caggccgcca gtcgatgctc gcgggtcgtt catggtgctc
     1261 cctcctgaca gtgctgctcc gaatgccgtc gttgcgttcg tttgactgat cagaaggtag
     1321 gcgtcgatct ggtgagcgag catcaccacg tctggtgatt tgggtggtga tttcacggaa
     1381 tcgcgcgacg aactccaccc ccaaccccct ccccgacgcg gggagggggt tgggggtggg
     1441 gtttgcgcct agcccttgcg cgagcgcgag agcaggtcca gttccgcggc gcggatgacg
     1501 gcctcgcggc ggttgttgac gccgagcttg ctgtagatgt ttttggtgtg ggtgcgcatc
     1561 gtgttcagcg agatgttcag ctcgcgggcg atgtcggggc cgtcgagggt ggttgcgagc
     1621 agccggagga catcgaactc gcggtcgctg agtggctcga tcaggtcttg cctgacaggg
     1681 gccatattct ctgtcatgtc ggaggctggc agcagctggc ggacggcgtt cggggcgatc
     1741 ccctgttgcg cggcggttgc caacagggcc gtcatcggcg gaccctcgtc gaggaagacg
     1801 cgggcgtagc cctccggctc ggccagcgtc agcgcccgtt ccaacggcag cagcgcggcc
     1861 ggggtattgc cggtcacctg ctgggcgagt gcttgaagga tgaggagttc gatgacactc
     1921 cccattctgc cgccggcttc ggccgcgtcc aggatgcggt gcagcacgtc gagcgcctcg
     1981 gtcgtggatt ggcgggccag tagcagccgc gccagcgtga tgtgctcgaa ctcgcgcagg
     2041 tagctgaggt cgtcgtcggt cgagaggttc tgatcccgcg cccagccgag ggcgtcgccg
     2101 atctgcccct gcctgatccg gacacgtgcc cgcagcgccg cgatcgggcg cacgttgggg
     2161 aagaactccg gcatccgcag gcgttcagcc tcgttgaggt aggccagcgc gccatccaga
     2221 tcgccttgcg actccttgat gcgggccatc atcacgttcc agcgagaccg gttgagcgtt
     2281 gcgcccgggc cgcgagccaa ctcctgggcg cgcagcaggt agtcggtggc cgcgtccagg
     2341 tcgttgcgct cgcgccggag ctcgctcagc ccgaccatga agtcggccgt cccatgcaga
     2401 cccggctcgc cctgctcctc ggcgcg
//
LOCUS       DASUGJ010000212         4377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1002768, whole genome shotgun sequence.
ACCESSION   DASUGJ010000212 DASUGJ010000000
VERSION     DASUGJ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002768"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..460)
                     /locus_tag="VFV93_04670"
     CDS             complement(83..460)
                     /locus_tag="VFV93_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164669.1"
                     /translation="MLQILRVVAVALFTLVAGSVGYAYASSNSGLTGAGGDGAGQISG
                     YQISDVSYTLNALDPTRIDAVSFDIDAGDSSPDVMVSLVRDGGAWHDCTVSTASAPAR
                     ATCQMRTPVAVVDVDELRVVAAQ"
     gene            982..2805
                     /locus_tag="VFV93_04675"
     CDS             982..2805
                     /locus_tag="VFV93_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5164670.1"
                     /translation="MPGRGDTDLRLDLLRHSLSRRGLLKGSTALAGGFALSGLLAACG
                     SDDDDGDDDGGGTGTTPEATSATGGATSATGGATSATGGATEPAEGSPEATEATDEGE
                     SGGGQPGGTLIIAFDADPESLDPHVTTALLASRVLAFMHDQLVVRDYDGQYVDDLASE
                     WEVSEDGLTYTFTLKPSVSFHSGKAMTSADVKWTFERWLATEASPTSYTIEPIDTIET
                     PDDATVVFHMKEPYNIFFDQLSGGWAVILNQEAVEAAGDTYGVDTVDGTGPFKFDSWT
                     RNQALKMTRFDDYSWGSSMFANQGPAYVEAAEVRVIPEANTAIAEFESGNVHLVASVP
                     PVEVDRLSNSSGVEIVKFDQLQTTYFGMNTTKAPVDSVEVRKAIGYGIDRESIVEGAW
                     FGLGTPALTMLHPDTPSFSQEALDAAPHFDPDQARSILEDAGWVEGDGGVREKDGQKL
                     VVPFWVINDDATVLQAQILEEQMADIGVQLETQQYEQTAWFEAARSGNQTAYTVGVFY
                     STADVLYFYFYSEQTPAPNRFFYNNPDVDGWLNDSRSNPDDAAVAEDYANIQMQLTED
                     YPAAPLIHQIGTLGKLDSVQGVKVHSSRWLYRLLDISLEQD"
     gene            2814..3755
                     /locus_tag="VFV93_04680"
     CDS             2814..3755
                     /locus_tag="VFV93_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164671.1"
                     /translation="MTQYIIRRLLILPIVMFGVSVLVFLVLHLVPGNPAQVIAGPDAP
                     PETVERIEHELGLDRSLPEQYWRYISRVFQGDLGQSLRSKRPVMDDIMDALPNTLQLT
                     FVSMLITPFIAIPLGVIAAAKRGSWIDSALMLISMLGITAPVFAVALGMMWVFGFKLE
                     LLPISGYGGPFWTWAGLKSILMPAITLSVLSIAATARLTRSAMLEVLGEDYIRTARAK
                     GLANSTVLMRHGLKNAMLPVVTVLGLQVAGLLSGAVVTETVFAWPGLGRLAVYAIDSR
                     DFPVVQGTVLVISLIFVLVNLAVDLLYAFVDPRIHYG"
     gene            3812..>4377
                     /locus_tag="VFV93_04685"
     CDS             3812..>4377
                     /locus_tag="VFV93_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164672.1"
                     /translation="MSDQPDSTPSGAAALPGSARLDRVAPQRSEARRLLRAFLRQRLG
                     VVGLAIIVLFTLMAIFASQIAPYSWREQSIRDRFQNASTEHILGTDELGRDIFSRILY
                     GARYSLVIGVSAVFVSFAIGAAIGMVAGFYPKLDTPIMRLIDVLMAFPGILLAIAIVA
                     ALGPGLRNVIIAIAVNEIPGFARLTRSMV"
BASE COUNT          764 a         1429 c         1426 g          758 t
ORIGIN      
        1 cgcagcgaag gacctctccg cgttcagttg gtacggcgct caagcagaga ggtcctttgc
       61 tgcggctcag gacgacaagg ggttactgtg ctgccaccac cctaagttcg tcaacatcga
      121 caacggccac tggggtgcgc atctggcagg tcgcgcgggc cggagcagac gctgtcgaga
      181 cggtgcagtc gtgccaggct ccaccgtcgc gaacgaggct gaccatgacg tccggcgacg
      241 aatcgccggc gtcgatgtcg aagctgacgg cgtcgatccg tgtgggatca agcgcattga
      301 gcgtatagga aacatccgag atctggtagc cggagatctg gccggcgcca tcgccgccgg
      361 ctcccgtcag gccactgttc gaggatgcgt aggcgtagcc gaccgagccg gccacgagcg
      421 tgaagagcgc gaccgcgacg acgcgcagga tctggagcac tgtcggcctc cccaaaggcc
      481 gaccaggggt ggtgagccag gacatccgtg ccaacgccaa cccggcgctg ccgtgtccag
      541 caataccagt ggccggttac gccacttgct cccccaggcg gcatcggcgt ccaaagcacg
      601 ccgccgggat cttcgcatcc actggtcaga ctgattgtta ccgtttgtct tgacaccaag
      661 acatttcctg ccatcaagcg tacgttcaca catgcgaatc gtccataccc cagaagtccg
      721 cgtcaccggc agaccttggc accggcggta ccgacgatcg ggcggccgag cgggccgatg
      781 ggtacccccg aggggcccgt tcggcagcga aaacgctgca atcgaggtcg acacacgacg
      841 gtaatgacgt gaatgttcgt ggcgcgcgac ttgcggaaac gacgcccata tcgagataca
      901 ccggcgcgga gaggattggt gcggctgcct gcgatggcat gcgcttcaag aagatgccgg
      961 tcgaatgagg aggagtagac catgcctggt cgtggtgaca cggatctgcg tctggatctg
     1021 ttgcgacatt cgctgtctcg cagggggttg ttgaagggct cgaccgcgct cgccggcggg
     1081 ttcgcgctgt ccggattgct ggccgcctgc ggcagcgacg acgatgacgg tgacgacgac
     1141 ggtggcggaa cgggcacgac cccggaagca acctcggcca ccggtggcgc tacgtcggca
     1201 acaggcgggg cgacctcggc caccggtgga gcgaccgaac cggccgaagg cagccccgaa
     1261 gcgacggaag ccaccgacga gggcgagagc ggaggcggcc agccgggcgg gacgctgatc
     1321 atcgcgttcg acgccgaccc cgaatcgctt gacccacacg tgacgacggc gttgctcgcc
     1381 tcacgggtgc tagccttcat gcacgatcag ttggtggtgc gtgactacga cgggcagtac
     1441 gtcgatgacc tggcgagcga gtgggaggtc agcgaggacg gcctgaccta caccttcacg
     1501 ctcaagccca gcgtgagctt ccattccggc aaggcgatga cgtcggccga cgtgaagtgg
     1561 acgttcgagc gctggctggc gacggaagcc tcgccgacca gctacacgat cgagccgatc
     1621 gacacgatcg agacaccgga tgatgcaacg gtcgtcttcc atatgaagga gccctacaac
     1681 atcttcttcg accagctctc gggcggctgg gcggtcatcc tcaatcagga agcggtcgaa
     1741 gcggccggcg acacctacgg cgtcgacacc gtcgatggga ccgggccgtt caagttcgac
     1801 tcctggaccc gcaaccaggc gctcaagatg acccgcttcg acgactacag ttggggatcc
     1861 tcgatgttcg caaaccaggg gccggcctac gtcgaggccg ccgaggtgcg cgtcatcccc
     1921 gaggcgaaca cggccatcgc cgagttcgaa tccggcaatg tccatctggt cgcgtcagtc
     1981 ccgccggttg aggttgaccg gctcagcaac tcgtctggcg ttgagatcgt caagttcgac
     2041 cagctccaga cgacctactt cgggatgaac acgaccaagg ctccggtcga ttcagtcgag
     2101 gtgcgcaagg cgattggcta tgggatcgac cgcgagtcga tcgtcgaggg cgcctggttc
     2161 ggcctcggca cgccggccct gacgatgctg caccccgaca ccccgtcatt ctcgcaggag
     2221 gcgctggacg cggccccaca cttcgatccc gaccaggctc gctcgatcct tgaagacgcc
     2281 ggctgggtcg agggcgatgg cggcgtccgc gagaaggacg gccagaagct ggtcgtgccg
     2341 ttctgggtca tcaacgacga cgcgactgtc ctgcaggcgc agatcctgga ggagcagatg
     2401 gcggacatcg gcgtccagct cgaaacgcag cagtacgagc agacggcctg gttcgaggcg
     2461 gcccgctccg gcaaccagac ggcctacacg gtcggcgtct tctactcgac cgccgatgtg
     2521 ctctacttct acttctactc ggagcagaca ccggcgccga accgcttctt ctataacaat
     2581 cccgacgtcg atggctggtt gaacgattcg cgctcgaacc cggacgacgc cgccgtcgcc
     2641 gaggactacg ccaacatcca gatgcagctt actgaggact atccagccgc gccgctgatc
     2701 caccagatcg ggacactcgg caagctggat tcggtccagg gcgtcaaggt ccattcgtcg
     2761 cgctggctct accggctgct ggatatctcg ctcgaacagg actaggccgg cagatgacgc
     2821 agtacatcat ccgtcgcctg ctgatcctgc cgattgtgat gttcggtgtc tcggtgctgg
     2881 tcttcctcgt cctgcatctc gttccgggga atccggccca ggtcatcgcc gggccggacg
     2941 ccccgcctga gacggtcgag cggatcgagc acgagctggg gctcgaccgc tcgctgccgg
     3001 agcagtactg gcgctacatc tcgcgcgtct ttcagggaga cctgggccag tcgctgcgca
     3061 gcaagcggcc ggtgatggac gacatcatgg acgcgctgcc gaacacgctg caactgacgt
     3121 tcgtctcgat gctgatcaca cccttcatcg cgatcccgct cggcgtgatc gcggctgcca
     3181 agcgcggcag ttggatcgac agcgcgctga tgctcatctc gatgctgggc atcacggcac
     3241 cggtcttcgc cgtcgcgctc ggcatgatgt gggtctttgg gttcaagctg gagctgttac
     3301 cgatcagcgg ctacggcggg ccgttctgga cctgggcggg actcaagagc atcctgatgc
     3361 cggccatcac cctctcggtc ctctcgatcg cggcgacggc ccggctgaca cgctcggcga
     3421 tgctggaggt gctgggcgag gactacatcc ggacggcccg cgccaagggg ctggccaact
     3481 cgaccgtcct gatgcggcac ggcctcaaga acgcgatgct gccggtcgtg accgtcctcg
     3541 ggctgcaagt cgccggattg ctgagcggcg cggtcgtgac cgagacggtc ttcgcctggc
     3601 cggggctggg gaggctggcc gtctacgcga tcgactcgcg cgacttcccg gtcgtccagg
     3661 ggacggtcct cgtcatctca ctgatcttcg tgctcgtcaa tctggctgtc gatttgctct
     3721 acgcgttcgt cgatccacgg attcactatg gctagatgga tggccgtccg gctcgcaagt
     3781 gccggccggc gccctgacag gaacagcaac catgagtgac cagccagaca gcacgccgtc
     3841 cggcgctgcc gcgctgccgg gttcggcccg gctcgatcgg gtcgcgccac agcgctccga
     3901 agcgcgccgg ctgctgcggg ccttcctgcg ccagcggctc ggcgtcgtcg ggctggcgat
     3961 catcgttctc ttcacgctga tggcgatctt tgccagccag attgcgccct acagctggcg
     4021 cgagcagagc atccgcgacc gcttccagaa cgcgtcgacc gaacacatcc tcggcaccga
     4081 cgagctgggc cgcgacatct tcagccggat cctctacggc gcgcgctact cgctggtgat
     4141 cggcgtctcg gccgtcttcg tcagcttcgc gatcggggct gcgatcggga tggtcgccgg
     4201 gttttacccg aagcttgaca caccaatcat gcgcctgatc gacgtcctga tggccttccc
     4261 cggcatcctg ctggcgatcg cgatcgtggc cgcgctcggt ccgggcttac gcaacgtcat
     4321 catcgcgatc gccgtcaacg agatccccgg cttcgcgcgg ctgacgcgct cgatggt
//
LOCUS       DASUGJ010000213         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1975242, whole genome shotgun sequence.
ACCESSION   DASUGJ010000213 DASUGJ010000000
VERSION     DASUGJ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975242"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="VFV93_04690"
     CDS             complement(<1..1249)
                     /locus_tag="VFV93_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5164673.1"
                     /translation="MSLSDTSLRAIIDSRLPETGGPRKRVIVVGAGMAGLVAAYELQR
                     AGHEPVVLEAQQRVGGRVYTLREPFSDGLYAEAGAMRIPRSHDLTMGYIAHFGLPIAD
                     FTMDNMNGWVYLHGQKWRISDVAADPAQLGFEVADTERGRTHSDLWNAALKPLADRLA
                     ADPVTAWQEIVSEYDRYSTREFLEANNWSEAAIEMFGLLADQEALMNSSFLELLREET
                     GRYYTDMVEVVGGSDHLPRAFLPALRDRIRFGARMIAIEQSPEDVTVHYQTAGQRFSV
                     SGDELIVTVPFPVLRHIEVLPEFSRGKQRAIRQLHYDASAKILLQFRRRFWEEIDGIY
                     GGGCFSDLPIRVTYYPDHGAETGRGVVLASYTWSEDAQRWGSLAPADRISQALENLAR
                     IHPEAIESFEVGASKMWHDDEFAG"
     gene            complement(1277..2557)
                     /locus_tag="VFV93_04695"
     CDS             complement(1277..2557)
                     /locus_tag="VFV93_04695"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164674.1"
                     /translation="MAFTGRVVDSARTGFIQAFASLAVPQYRLLWLGMLFSIGAMQID
                     LVARAWLAYDLSDSGLTLGLVTAARGLPQLLLSPYSGVAADRYDKRKLLVWSQSAVFV
                     LAAINAVLVHTGVIQIWQLALLGLAEGIVSPFTLPTRTALIPDLVEERQIPNALALES
                     TGRNINRIVAPAFAGVCLSISPALAFYAIAVFYGLAVLTLYQLPRGLRGEGGQKGAMG
                     EMMEGFRYIWERPSLFALLALAYVPILLGMPFQSLLPVFQKDVLGISESALGLMYTAI
                     GIGAIIGSVIIAAIAESPHKNRIQIISGIIFGIVLFLFALSSNYTLSLVLLLLVGFFS
                     TGYLTINRMLVGLQTERGMYGRVMATYGMTWSLMPISLLPFGALVDIFGVSATVAVSG
                     LVVAVFISVIAIVFARLFLGTPEPSRYEGAGIRD"
BASE COUNT          518 a          942 c          954 g          449 t
ORIGIN      
        1 cgccggcgaa ctcgtcgtcg tgccacatct tcgacgcccc gacctcgaag gattcgatcg
       61 cctccgggtg gatccgggcg aggttctcca gtgcctggct gatccggtcg gccggcgcca
      121 gcgagcccca gcgctgggca tcctccgacc aggtgtagct ggcgaggacg acgccgcggc
      181 cagtctcggc gccgtggtcg gggtagtagg tgacgcggat cggcagatcg ctgaagcagc
      241 ctccaccgta gatgccgtcg atctcctccc agaagcgacg gcggaactgg agcaggatct
      301 tggccgaggc gtcgtagtgc agctggcgga tagcgcgctg cttgccgcgc gagaactccg
      361 gcagcacctc gatatgccgc aggaccggga acggcaccgt cacgatcaac tcgtcgccgc
      421 tgacgctgaa gcgctgcccg gccgtctggt agtggacggt gacgtcctcc ggcgattgct
      481 cgatggcgat catccgcgcg ccgaagcgga tcctgtcacg cagggcaggc aggaaggcgc
      541 gcggcagatg gtctgacccg ccgacgacct cgaccatgtc ggtgtagtag cggcccgtct
      601 cctcgcgcag cagctccagg aacgaggagt tcatcagcgc ttcctgatcg gccagcagcc
      661 cgaacatctc gatcgcggct tcagaccagt tgttcgcctc caggaactcg cgcgtcgagt
      721 agcgatcgta ctcactgacg atctcctgcc aggccgtcac cggatcggcc gccagccggt
      781 ccgccagcgg cttcagcgcc gcgttccaga ggtcgctatg cgtccgcccc cgctctgtgt
      841 cggcgacctc gaagccgagc tgggccgggt cggcagcgac gtcgctgatg cgccacttct
      901 ggccgtggag gtagacccag ccgttcatgt tgtccatcgt gaaatcggca atcggcagcc
      961 cgaagtgggc gatgtagccc atcgtcaggt cgtgcgagcg gggaatccgc atcgccccgg
     1021 cctcggcgta gagcccgtcg ctgaacggct cccgcagcgt gtagacgcgc ccgccgaccc
     1081 gctgttgcgc ttccagcaca accggctcat gcccggctcg ctgcaactcg taggcagcca
     1141 ccagccccgc catcccggcc ccgacgacga tcacccgctt gcgcggcccg cctgtctccg
     1201 gcaacctgct gtcgatgatc gctcgcaggc tcgtgtcgct cagtgacatg tcgttccctt
     1261 ctggcggtct attgggtcag tcgcggatgc cggcgccctc gtatctggaa ggctccggcg
     1321 tgccgaggaa gagtcgggca aagacgatgg cgatgacgct gatgaagacg gcgacgacga
     1381 ggcccgagac ggcgacggtt gccgagacgc cgaagatgtc gaccagcgcg ccgaagggca
     1441 gcagcgagat cggcatcagc gaccaggtca tgccgtaggt cgccatcacc cggccgtaca
     1501 tgccgcgctc ggtctgcagg ccgaccagca tccggttgat cgtcaggtag ccggtgctga
     1561 agaagccgac cagcagcagc aggaccagcg agagggtgta gttcgacgag agcgcgaaca
     1621 ggaacaggac gatcccgaag atgatgccgg agatgatctg gatgcggttc ttgtgcggtg
     1681 attcggcgat ggcggcgatg atgacggagc cgatgatcgc gccgatgccg atcgccgtgt
     1741 acatgagccc cagcgccgac tcgctgatcc ccagcacgtc tttctggaag accggcagca
     1801 gcgactggaa cggcatcccc agcaggatcg ggacatacgc cagcgctagc agcgcgaaga
     1861 gcgatggccg ctcccagatg tagcggaagc cctccatcat ctcccccatc gcgcccttct
     1921 ggccgccctc gccgcgcagg ccacgcggca gctggtagag cgtcaggacg gccagaccgt
     1981 agaagacagc gatggcgtag aaggccagcg cggggctgat cgagaggcag acaccggcga
     2041 aggccggcgc gacgatgcgg ttgatattgc ggccggtcga ttcgagcgcc agcgcgttgg
     2101 ggatctgccg ctcctcgacc aggtcgggaa tcaacgctgt ccgcgtcggc agcgtgaatg
     2161 ggctgacgat cccttcggcc agcccgagca gcgccagttg ccagatctgg atgacgccgg
     2221 tgtggaccag gacggcgttg atcgccgcca gcacgaagac ggccgattgc gaccagacca
     2281 ggagtttgcg cttgtcgtag cggtcggccg caacaccgct gtacggcgag aggagcagtt
     2341 gcggcaagcc ccgcgcggcc gtcaccagtc ccagcgtcag gccggagtca gagagatcgt
     2401 aggcaagcca ggcacgggcg acgaggtcga tttgcatcgc gccgatcgag aagagcatcc
     2461 ccagccagag cagccggtac tgcggcaccg ccagcgaggc gaacgcctga atgaagccgg
     2521 ttcgcgcgct gtcgacgacc ctgccggtga atgccacaca acctcctgcg tctgtctgtc
     2581 gcgccctatt atgcgccgag gcggcaaaca tcgctggaac gccgcagccg atcatgcttc
     2641 gctggcgacg acgcggagtc ggttgcgata ccctgtatgt caggcggcaa gcagttccga
     2701 aaggtggccc gatcatgcag aaaaccaccg tctacctgcc tgccaacctg gttgggtggc
     2761 ttcgcgaagt gtcgcggcaa acgggtcgat cccaagccga actgttgcgt gaggcgctcg
     2821 aaatctatgc cgcgcaacaa ccgcgactgc tgccgatctc gat
//
LOCUS       DASUGJ010000214         3768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1418703, whole genome shotgun sequence.
ACCESSION   DASUGJ010000214 DASUGJ010000000
VERSION     DASUGJ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418703"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VFV93_04700"
     CDS             <1..1277
                     /locus_tag="VFV93_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164675.1"
                     /translation="PRSLPIRAAQQRQSRIRYAVAAIVAVALLCGSVLSGTFWLGSSG
                     EVSAQEIIARASVPALASGDADIQSFVITESRTINTDDGRRKVQMTRWYDARDHWRIE
                     SEETVTTETGSSVRHFGAVADGQLIWDWTDDSARVREIPADQQDLLDLAAFGGGWSDL
                     SDALQSASECATPERQPDATVAGRAVYVIDLGESTCPSASMPATNGRRVVSIDKDTFF
                     VLKVVQYDGRPDHAGQVYSTTEVTSVDYNVALEADLFTYSPPAGLPVDDQRVTATATA
                     PATVTPQPEPTSQPSSLDALREQVSYPIFVPTIIPDGLTATEPTGGQAADSFVEIEYQ
                     TADGQPGLSVLVGPAGCCLAQAGKDGYAVTLSNGLAAHNVASQPDSPAGILWWNQDGA
                     YIALSGTNLTFDDLLQIAASMSSSAELDGTGS"
     gene            complement(1308..2177)
                     /gene="sucD"
                     /locus_tag="VFV93_04705"
     CDS             complement(1308..2177)
                     /gene="sucD"
                     /locus_tag="VFV93_04705"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5164676.1"
                     /translation="MSILVGKDTRLLVQGITGREATFHTGQMVEYGTNVVGGVTPGRG
                     GKVVHGAPVFDTVAEAVERTGANTSIIFVPAAFAPDAILEAVDNGIGLVICISENVPA
                     LDMVKVYAHVKARGARLIGPNCPGLITPGEAKVGIMPGYIHERGRVGLVSRSGTLTYE
                     VVWALTQAGLGQTSAVGIGGDPIIGTSFIDVLELFENDPETEGIVLLGEIGGTDEDQA
                     AAYIKEHVTKPVAGFIAGRTAPPGKRMGHAGAIISGGAGTAAEKIAALNDAGVDVADT
                     PAGVADLMKARLS"
     gene            complement(2268..3407)
                     /gene="sucC"
                     /locus_tag="VFV93_04710"
     CDS             complement(2268..3407)
                     /gene="sucC"
                     /locus_tag="VFV93_04710"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX5164677.1"
                     /translation="MNIHEYQAAELLAGFGIPVNPGEMVTDAAAAGKAAARLAGPGGK
                     VALKAQVHSGGRGKAGGIKLASSPDEATELAGQILGMDIRGHTVRRLLVVPAVDIARE
                     YYLGIILDRAARGVTLMASAEGGVEIEEVARETPEKIVKITADPLLGLLDYQVRQVAF
                     GLGIEPALMSGFVAIAKSLYTAFVSSDASLAEINPLILTKDERWLALDSKVSLDDSAL
                     PRHPQFEQLRDMDEENATELRAKANGISFVKLDGNIGCVVNGAGLAMATMDACKLYGG
                     EPANFLDVGGGASAEQVTLALELILEDPAVKAIFFNIFGGITRCDVVARGILAALDQI
                     KVDVPMVIRLVGTNQEEGRQILAEAGITALDTMAEAAQAAVAAAK"
BASE COUNT          596 a         1313 c         1251 g          608 t
ORIGIN      
        1 cgccgcgttc gctgccgatc cgggcagcgc agcagcgaca gtcgcgcatc agatatgcag
       61 tcgcggccat cgtcgccgtt gcgctgctgt gcggctccgt cctctccggc acgttctggc
      121 tggggtcgtc cggcgaggtc agcgcccagg agatcatcgc ccgggcgtcg gtccccgcgc
      181 tcgcgtccgg cgatgccgat atccagagct tcgtcatcac cgagagccgg acgatcaaca
      241 ccgatgatgg ccggcgcaag gtgcagatga cgcgctggta cgacgcccgc gatcactggc
      301 ggatcgaatc cgaggaaacc gtcacgaccg agacgggcag cagcgtcagg catttcggcg
      361 ccgttgccga cggtcagctg atctgggact ggaccgacga ctcggcccgc gtccgtgaga
      421 tcccggccga ccagcaggac ctgcttgacc tggccgcctt cggcgggggc tggagcgacc
      481 tcagcgatgc actccagtca gcgagcgagt gcgcgacacc cgaacgccag ccggatgcca
      541 cggttgccgg gcgggcggtc tacgtgatcg acctcggtga gtcaacctgc ccgtcggcct
      601 cgatgccggc gacgaatggc cggcgcgtcg tctcgatcga caaggacacg ttctttgtgc
      661 tcaaagtggt gcagtacgac gggcggccgg accacgccgg ccaggtctat tcgacgaccg
      721 aggtcacgag cgttgactac aacgtcgcgc tggaagctga tctgttcacg tactccccac
      781 cagccggcct gccggtcgat gaccagcgcg tgacggcgac ggcgaccgcg ccggcgaccg
      841 tgacaccaca gccggagccg acctcgcagc catcgagcct ggacgcactg cgcgagcagg
      901 tgtcgtaccc gatctttgtc ccgaccatta tcccggatgg gctgactgcg acggagccaa
      961 ccggcgggca ggcagccgat tcgttcgtgg agatcgagta ccagacggct gacggtcagc
     1021 ctggcttgag cgtgctggtc ggtccggccg gctgctgcct ggcgcaggcc ggcaaggatg
     1081 gctacgcagt gacgctgtcg aacgggttgg ccgcccacaa cgtcgccagc cagccggaca
     1141 gccccgctgg aatcctctgg tggaaccagg acggcgccta catcgcgctc tccggcacga
     1201 acctgacctt tgacgacctg ctccagatcg ccgcctcaat gtcgagtagc gccgagctgg
     1261 acgggaccgg atcataagct ccaacgacgc ggcaacggag ccgcctgtca ggagaggcgg
     1321 gccttcatca ggtccgccac cccggccggg gtatccgcca cgtcgacacc ggcgtcgttg
     1381 agggcagcga tcttctcggc cgccgtgccg gcgccaccgg agatgatggc gccggcatgg
     1441 cccatccgct tgcctggcgg ggccgttcgg ccggcgatga agcctgcgac cggcttggtg
     1501 acgtgctcct tgatgtacgc cgctgcctgg tcctcatcgg tgccgccgat ctcgccgagc
     1561 aggacaatcc cctcggtctc ggggtcattc tcgaagagct ccagcacatc gatgaacgag
     1621 gtcccgatga tcgggtcgcc gccgatgccg acggccgagg tctggcccag cccggcctgg
     1681 gtcagcgccc agacgacctc gtaagtcagc gtgccggagc gggagacgag gccaacccgc
     1741 ccgcgctcgt gaatgtagcc gggcatgatg ccgaccttgg cctcgccggg cgtgatcagg
     1801 cccgggcagt tcgggccgat cagccgcgcg cccctggcct tgacgtgcgc gtagaccttg
     1861 accatgtcga gagccgggac gttctcggag atgcagatga ccagcccgat cccattgtcg
     1921 accgcctcaa gaatcgcgtc cggcgcgaag gcggccggaa cgaagatgat cgacgtgttc
     1981 gcgcccgtcc gctcgaccgc ctcagcgacc gtgtcgaaga ccggcgcacc gtggacgacc
     2041 ttgccgcccc gccccggcgt cacgccaccg acgacgttgg ttccatactc aaccatctgg
     2101 ccggtgtgaa acgtcgcctc acgccccgtg atgccctgca ccagcagccg tgtgtcctta
     2161 ccaacgagga tgctcactct ttcccccttc gtaacgcgtg acgcgtgatg cgtgacgcga
     2221 tggtccgaac cagacccgcg tcccgcgccc catgtcccgc gtcccgccta cttcgccgct
     2281 gccacggctg cctgggccgc ttcggccatc gtgtcgagcg ccgtaatgcc ggcctcggcg
     2341 aggatctggc gtccctcttc ctgattggtg ccgaccagcc ggatcaccat cgggacatcg
     2401 accttgatct ggtcgagcgc cgccaggatg ccgcgcgcga cgacatcgca gcgggtgata
     2461 ccgccgaaga tgttgaagaa gatggctttg acggccgggt cttcgaggat cagctccagc
     2521 gccagcgtca cctgctccgc gctggcgccg ccgccgacat cgaggaagtt ggccggctcg
     2581 ccgccgtaga gcttgcaggc gtccatcgtc gccatcgcca gcccggcgcc gttgaccacg
     2641 cagccgatat tgccgtccag cttgacgaac gagatgccgt tcgccttggc gcgcagctcg
     2701 gtcgcgttct cctcgtccat atcgcgtagc tgctcgaact gcgggtggcg ggggagggcg
     2761 ctgtcgtcga ggctgacctt cgagtcgagc gccagccagc gctcgtcctt cgtcaggatc
     2821 agcggattga tctcggccag cgaggcgtcg gaggagacga aggcggtgta gagcgatttc
     2881 gcgatcgcga cgaagccgga catcagcgcc ggctcgatcc ccagcccgaa ggcgacctgg
     2941 cgcacctggt agtccagcag ccccagcaag gggtcggccg tgatcttgac aatcttctcg
     3001 ggcgtctcgc gggcgacctc ttcgatctcg acgccgccct cggcgctggc catcagtgtc
     3061 accccacggg cagcgcggtc gaggatgatg ccgaggtagt actcgcgggc gatatcgacg
     3121 gccgggacga ccagcagccg ccggacggtg tggccgcgta tgtccatccc gaggatctgg
     3181 ccggccagct cggtcgcctc atccggcgag gatgccagct tgatgccgcc ggccttgccg
     3241 cgcccaccgc tgtggacctg cgccttgagg gcgaccttcc cgcccggccc ggccagcctg
     3301 gcggcggcct tcccggcagc ggcggcgtcc gtcaccatct cgccggggtt gacgggaatc
     3361 ccgaaaccgg cgagcagttc ggcggcctga tactcgtgga tgttcattgg cgtgcgtccc
     3421 tccggagcgg agctgccagc cttcagccgt cagcctgctg gttgcgggtg aatgtgctcg
     3481 tcacccggcc aacgacatga gctgatgtcc agcagtgacg cgtccgggcc acccaatcga
     3541 ggatgatagc acgcacgccg gatgccgcgt gctgacagct tcagcacgcg gtatccacgc
     3601 tgctggctgc tactggcagc cctcagccat ccgtgcggca gcgtcggcgt cgctcgacac
     3661 ttggctgacg tcagccgagg cgatcgccag cggcgagtag ggcggctcaa cctgcagctc
     3721 cggccgggcc agatgcagca cgtagcagcc ggcgaagatc tgggcgcc
//
LOCUS       DASUGJ010000215        14538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1418913, whole genome shotgun sequence.
ACCESSION   DASUGJ010000215 DASUGJ010000000
VERSION     DASUGJ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14538
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418913"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..684
                     /locus_tag="VFV93_04715"
     CDS             235..684
                     /locus_tag="VFV93_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164678.1"
                     /translation="MAKRTYLWCDNCRRSFDFPDAPGGLCPICSTQMRELGWLNAFVR
                     GVMAQELVASGLPSRHRTLIRMIWTANGMGERYYKALAPPVTYSKFEGRVTEYVCAAA
                     SEGWVRFVLPPSPIGAADDAYRMEIDDEERFISEMAALFADDESGTG"
     gene            complement(670..1794)
                     /locus_tag="VFV93_04720"
     CDS             complement(670..1794)
                     /locus_tag="VFV93_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5164679.1"
                     /translation="MPVETTDRAERLRQYRTMLVIGVILVIAWLVWSSRGALLPFAIG
                     LIFSYVIAPLVNRVQMAIPNRGWLGRARRGIAVMIVYLSVLGLIVIAVATVGPRLVNE
                     TFDLVEGLPQYAETVREESNYWNRWYEETVPENVRVEIEAHLDEIGTVVAGAARSAIM
                     ATFGTIQRVFGFLAGLLLLPLWTFYILKDQRRGMDFFYNMFPPNIRPDVRNVVGIADR
                     ILGAYIRGQLLLGLAVGIVTFIGLYVLDVQYAVPLAVLAGIFEMVPILGPWISGTVAA
                     IVVLATDPGKIWAIIILFVMIQQVENTFLVPKIQGDAVDLNPAIIMILLVVGGAVFGL
                     LGVVVIVPAAAIARDVFIYVYGRLSEESNRIDATESSAGP"
     gene            complement(1912..3705)
                     /gene="thrS"
                     /locus_tag="VFV93_04725"
     CDS             complement(1912..3705)
                     /gene="thrS"
                     /locus_tag="VFV93_04725"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX5164680.1"
                     /translation="MVSQAAAPDEMQVQPENEAEFDLARMRHSAAHVMAEAVLELFPD
                     AKLAIGPAIENGFYYDFDLPRSLSPEDLEQIEANMRTHIKAREVFERREVSRDEALSI
                     FADQPYKLELIENLPADEVISTYQNGPFLDLCRGPHVRDTSNIKAFKLLNIAGAYWRG
                     DEKRPMLQRIYGTAWPSKDDLDAYLNQLEEARKRDHRRVGRELGLFTLSEDIGQGIPI
                     FFPKGEMVRYLMESYVRDVQTRYGYQHVWTGHLVKEELFARSGHLEHYSDVMFPVMED
                     EGVRMRLKPMNCPSHMTLYNQQLHSYRELPLRYAEFATLYRYEKSGELSGLTRVRALT
                     QDDCHIFCTEEQIGEEFGRALDLIREILDTYGFNDYYIQLSLRGDEGKYVKDDEKWSR
                     AEQALRGAMDSKGVAYVPATGEAAFYGPKADFMARDVLGREWQLSTIQVDFIQPERLG
                     CEYIGEDGQPHTPVVIHRAVTGSTERFLGVLIEHFDGAFPVWLAPVQAVIIPIADRHA
                     EYALQAGDRLRAAGLRVEVDTGGDRMQNKIRQAQNQKVPFMLVVGDKEIEADAVAVRL
                     RSGENLGAVSVADFIDRANRVVASRSLDLQL"
     gene            complement(4112..4188)
                     /locus_tag="VFV93_04730"
     tRNA            complement(4112..4188)
                     /locus_tag="VFV93_04730"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4152..4154),aa:Val,seq:gac)
     gene            complement(4267..4893)
                     /locus_tag="VFV93_04735"
     CDS             complement(4267..4893)
                     /locus_tag="VFV93_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164681.1"
                     /translation="MKWIVAGHWTALRIAAFVVPQATLFFRRDRNRPTRADAYRDLFS
                     SLESLYESAARLRPEWRDLWRDRRTQPLPPKYESGDEMTTNAVHDMRYATWLLQRYRW
                     KSVVKPLFEDIDEPAWSRFSATASDISPSLLALSDAHLERLREDEIDWINTAVEQFDE
                     ATRRRRRLTDTDAPRAQQVAEGVYQPIYIAIHLSDRLIERLRYEASQR"
     gene            complement(4890..5399)
                     /locus_tag="VFV93_04740"
     CDS             complement(4890..5399)
                     /locus_tag="VFV93_04740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5164682.1"
                     /translation="MNTSFLSPAVAVGDSFPPFELRSCRDRPVAIDPRSALIIFYRGH
                     WCSHCRGQFADLARQHDSFRSLNFSIIAISADDFVGANDMTVDTGGAIDFLSDPSAAF
                     IEQLGLVDRDDSVDHVIARPAVFIIDADGIVRYRYISRSPADRPTSALLLLAIESLAH
                     PARKESTTR"
     gene            complement(5396..5845)
                     /locus_tag="VFV93_04745"
     CDS             complement(5396..5845)
                     /locus_tag="VFV93_04745"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX5164683.1"
                     /translation="MTGVSRRPTPPTRSAPDADVTLVFDGGSLGNPGKGYGSFVYTGV
                     IETERPVRLDYPGQTTNNEAEYQTMLHGLRTILRELERDARDPATMRVAVLSDSKLVV
                     EQVNGRWKVRHAPLRPFREEALALLSRFGSWHLVWQPRIESVRLLGH"
     gene            complement(5908..7986)
                     /gene="fusA"
                     /locus_tag="VFV93_04750"
     CDS             complement(5908..7986)
                     /gene="fusA"
                     /locus_tag="VFV93_04750"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5164684.1"
                     /translation="MKTYPVESIRNIGLFSHGGAGKTSLTEAMLFTGKAISRLGRVEE
                     GNTVSDWDPDEIKRTISISATPAPVEWKDHKINIIDAPGYADFVGEVKAAIRVVDTAV
                     ILLDASAGVEVGSEYAWRFAAERNLPAIVIINKMDRENANFAASLASAQAAFGQKVVP
                     LYIPIGSESALRGVVDLLGMRAWMFTESRDGTVTEADVPAEMLDEVETYRLQLVEKIA
                     ENDEELMLRYLEDDEISADELRAGLRAGIASGEIVPVLSGAASSNRGTSVILDAIIDL
                     APAPGPTAAKTPEGGEVTLTPDPNGPIAAFIWKTIADPFVGKLTYFRVYSGTLKSDSH
                     IWNVTRNKDERAGQLYLLRGKEQIAVNEVCAGDIGAVAKLVETTTGDTLADPQHKVLI
                     DGIEFPQPLFTAAVAPKTKSDLDKMGTALQRVADEDPTLHIGRDAATGETVVSGLGES
                     HVQIAMERMARKFGVNVDIGLPVVPYRETIQKAVQHIEYKHKKQTGGHGQYGHVFIDV
                     EPAEGEFQFEEKIFGGSVPRQYIPAVEKGLREAMEEGILAGFPVVNVKVTLTDGSYHN
                     VDSSEMAFKLAASQAFKKAAQSANPVILEPVLNVSVTVPDAYTGDIMGDLTVKRAHVS
                     GMTPSGDGYTTIEATVPASEVQRYATDLRSLTQGRGTFTTEFSHYQQVPSHLTDSIIA
                     AHRARQEANA"
     gene            8214..9320
                     /locus_tag="VFV93_04755"
     CDS             8214..9320
                     /locus_tag="VFV93_04755"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5164685.1"
                     /translation="MVERDAMRQRIKDLLHDLSGLDGVSGHEHAVVQRLVELLSPHAE
                     TTVDPYGNVFARVNDAGSGPSLMVAAHSDEIGALVKSIEPNGMIRFERVGGVIETLLI
                     GRHVRIRGLRGIVGVKAGHVQSADERGRAPAIRELYIDMGFDSAAEVEALGIRVGDAI
                     AYDEPMEELANPVRVSGKALDNRVSCALVVLLAERLRGVTLGCTLHVVVTVQEEVGLR
                     GARIATHRLNPDAAIVVDTVPSGGTPDVDFHKDLRMRIGGGPVLGLASSGGSAGHHIN
                     PAMRDFLRRTAADAGITLQEAVFYGGTSDASAVHLVHDGIPTGVINIARRYSHSPVET
                     LDLNDAVDTLLLLEAVARAFNAGTDFGFLAAEWG"
     gene            complement(9325..10101)
                     /locus_tag="VFV93_04760"
     CDS             complement(9325..10101)
                     /locus_tag="VFV93_04760"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5164686.1"
                     /translation="MLLLVDIGNTNVKFGIDIDGELHSRWRVHTDRTSMPDEWWIVLN
                     TLAVADGIDLTAIDGAVVSSSVPVVTPWLTTMIRDRIRIEPVIVGPTTDLGITVEIDN
                     PTEVGPDRLVDAAAAFDRFGGPVVVLDFGTATTLNIVNERGAFIGGAIAPDLRSAHDA
                     LVGKAAKLTSVDLVFPASVIGHNTVAAMQAGIMLGYVGLINGLIAQIDEELGEQPTVV
                     STGGFGGVFKDRCPRISTFAPDLTIDGLRLVWRRHLQSAR"
     gene            complement(10143..11330)
                     /gene="queG"
                     /locus_tag="VFV93_04765"
     CDS             complement(10143..11330)
                     /gene="queG"
                     /locus_tag="VFV93_04765"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX5164687.1"
                     /translation="MTTFDLTRTDLTTLATAANLTITGVTDGAPFRDVGAYVVDHVER
                     GHMHGMDWFTVERVRQSADPRTLHDSVRTIVSVGVPFWSGQANPPGDGVLRGRIARYA
                     WGRDYHNTLKRRMRDLVAAIEQRVGRTVEARVLVDTARVVDRAVAARSGAGWYGKNSM
                     IIVPGHGSWVMLGEIMLDITIPSDVPMAKDCGRCQICIDRCPTGAIVEPYRIDAPRCI
                     SYLTIEHRGVIPHHLRPLMGNFVFGCDICQDVCPYTSAARHVDDADFAPRSVDNAFPS
                     LEFLATMSEEQFRATYSGTPVTRAKRAGMARNAAIALGNSRDPGAEPILIAMLLNHDE
                     PLARGHAAGALRQLVGEDAKRTLRAAWSRERESYVREEIERGLEPKFAVAPAETAVDL
                     HPR"
     gene            complement(11327..12271)
                     /locus_tag="VFV93_04770"
     CDS             complement(11327..12271)
                     /locus_tag="VFV93_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX5164688.1"
                     /translation="MRRGVAAIGLCSWDRFVVTDDYPGPGEYAVVRHQLEQAGGTTGN
                     TCHALAKVGVNVLLVSVVGDDPEGAALIDSLRDAGCDTGAIAIREGARSDTGIIIVSG
                     PPSQRDRTIYWIQGVKPLMGDPLPVDEMLEHEWVHIDIDDPRLRSFFLDLPAHRSPRT
                     KLFGTMTYLVEMPPGAGWQHALRFDAVTGNERELRTLLGAESLDAAIDQVQRDLVASA
                     CQVVFLSRGPYGAIAIRESGVELAPAVAIEVIDTTGAGDAFAAGCLWGLLDRLDDADI
                     LRRANALGGLACRALGARAGLPTRAEVERVLSGHELPG"
     gene            12391..13080
                     /locus_tag="VFV93_04775"
     CDS             12391..13080
                     /locus_tag="VFV93_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5164689.1"
                     /translation="MADASLIVVEDEPALADALRFNLERQGYDVSVITDGHAAVREIG
                     RSQPDLVILDVMLPGLDGFEVCRRVRLHSTVPILMLTARGEEVDRVLGLEIGADDYLT
                     KPFSLRELQARVRALLRRSSAQQPRERSEVRQVGNLRISDAERRAWLDEQELQLRPRE
                     FDLLSFLVRNQSLALTRQQILDGAWERDFAGDERTVDVHVKALRDLIEDDPRQPVRIV
                     TVRGVGYRFEG"
     gene            13085..14452
                     /locus_tag="VFV93_04780"
     CDS             13085..14452
                     /locus_tag="VFV93_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5164690.1"
                     /translation="MRGLSIQFMLPDFVVLAALVIGWLAGSTGWGVGVGGSLALVVAI
                     GLARRAERRLEHLERMVRPDAGLDLADAATIDVHDQIDHAIRTVAQQALHQRTALDAM
                     ELNLTQQAQVLDQMNDGLMRVALDGRVTYANVAAGSLFGGRNPTGRTFMSVTHDHELN
                     RAVRACLESGNVQHHTLEIPGEGRLVDAVAIRLSERPIEVLVMLRDITEVARLQNLRR
                     DFVSNVSHELRTPLSTIKILTETLLDIQAGDSEASGFLQQIDGEVDSMTALVKDLLDL
                     TRLETTGSALALRDVDAALLVTDAVDRMRPLARRHDVTLDVDIGDGAGHLAADERRLL
                     QAMINLISNAIVHTESCGTVTAGARREPGGVRFFVRDTGWGIPPDDLPRIWERFFKAD
                     RARTGPGTGLGLAIVKHIVQAHGGSVAATSELGHGSEFSFEIPDNLEPVAKLPPAVEL
                     PVAPN"
BASE COUNT         2526 a         4364 c         4981 g         2667 t
ORIGIN      
        1 agcgcccaga gcgcgagccc gcccgataga atcaggaggg tggtatagag ggccgagtgg
       61 atctccagca cgtgttcgtc ccttccgggt tatcgctgac gtggcgcacg gatgcgaccg
      121 ttcgatcggc tgcctggcag aatcgtgtgg gtggggtttt cagccgtcaa cacgttatca
      181 acttcggcag gctgcgacaa ggcacgcagc cggaaatggg atgcgatcgt aggaatggcg
      241 aagcgaacct acctctggtg tgacaattgc cgccgctcgt ttgacttccc ggatgcgccc
      301 ggcgggctgt gtccgatctg ctcgacgcag atgcgtgagc tcggctggct gaatgcgttc
      361 gtgcgcgggg tgatggccca ggagttggtc gcgtctgggt tgccgtcgcg ccaccggacg
      421 ctgatccgga tgatctggac cgcgaacggt atgggcgagc gctattacaa ggcgctggcc
      481 ccgccggtga cgtacagcaa gttcgaggga cgcgtgacgg aatatgtctg cgcggcggcc
      541 agcgaggggt gggtgcggtt cgtcctgccg cccagcccga ttggcgctgc cgacgatgcc
      601 tatcggatgg agatcgacga cgaggagcgc ttcatcagtg aaatggcggc gttgttcgcc
      661 gacgatgagt cagggaccgg ctgacgactc ggtcgcatcg atgcgattgc tctcctccga
      721 gagccggcca tacacgtaga tgaagacgtc gcgggcgatg gctgcggccg ggacgatcac
      781 gacgacgccc agcagcccga agacggcgcc gccgacgacc agcaggatca tgatgatggc
      841 cgggttcaga tcgacggcgt cgccctggat cttgggaacg aggaaggtgt tctcgacctg
      901 ctggatcatc acgaacagga tgatgatcgc ccagatcttg ccggggtcgg tcgccaggac
      961 gacgatggca gcgaccgtcc cggagatcca cggacccaga atcggcacca tctcgaagat
     1021 cccggccagg accgccagcg gaacggcata ctggacatcc agcacgtaga gaccgatgaa
     1081 cgtcacgatg ccgacggcaa ggccgagcag cagctggccg cggatgtagg ctcctagaat
     1141 acgatcggcg ataccgacga cgttgcgaac gtcggggcgg atgttcggcg ggaacatgtt
     1201 gtagaagaag tccatcccgc gtcgttggtc cttcagaatg tagaaggtcc agagcggcag
     1261 cagcagcagt ccggccagaa atccgaagac gcgctggatc gtgccgaagg tcgccatgat
     1321 cgcggaccgg gcggcaccgg cgacgaccgt gccaatctca tccagatgcg cctcgatctc
     1381 aacccggacg ttctcaggga cggtttcctc gtaccagcgg ttccagtagt tgctctcctc
     1441 gcgcacagtt tcagcgtact gcggtaagcc ttcaaccagg tcaaacgttt cattgaccaa
     1501 tcgcggaccg acagttgcga cggcgatgac gatcagcccc agcacggaga gatagacgat
     1561 catgacggcg ataccccgcc gtgctcggcc cagccagcca cggttgggga tcgccatctg
     1621 gacgcggttg accagtggtg cgatgacgta ggagaagatc aggccgatcg cgaacggcaa
     1681 cagcgcgccg cgcgacgacc agacgagcca ggcgatgacg aggatgacac cgatgacgag
     1741 catcgtccgg tattggcgca gccgttcggc tcggtctgtc gtctcaaccg gcaagaagct
     1801 gtctcctccc cgctatcgct ggccgcagga cgatacccga ttgtcggatt cgaggcaaca
     1861 gccccacgct ggatcgcgcg gggctgtcgc ttccgggttc aggcgacaat tctagagctg
     1921 caggtccagt gaccgtgacg caacgacgcg gttggcgcga tcgatgaaat cagccacgct
     1981 gacggctccg aggttctcac cggagcgcag gcgaacagcg acagcgtccg cctcgatctc
     2041 tttgtcacca accaccagca tgaacggcac tttctggttc tgagcctggc gaatcttgtt
     2101 ctgcatcctg tctcctccgg tgtcgacctc gacccgcagg ccggccgcgc gcagccgatc
     2161 tcccgcctgg agtgcgtatt cggcatgccg gtccgcgatc gggatgatga cggcctgaac
     2221 cggcgccagc cagaccggga acgcgccgtc gaagtgctcg atcagcacgc cgaggaagcg
     2281 ctccgtgctg cccgtcacgg cccggtgaat gacgaccggt gtgtgcggct ggccatcttc
     2341 gccgatgtac tcgcagccga ggcgctcggg ctggatgaag tcgacctgga tcgtcgagag
     2401 ctgccattcg cggccgagga catcgcgggc catgaagtcg gccttgggtc cgtagaaggc
     2461 ggcttcgccc gtcgccggca cgtaggcgac gcccttggag tccatcgcgc cacgcagcgc
     2521 ctgttcggcc cgcgaccact tctcgtcgtc cttgacgtat ttgccctcgt caccccgcag
     2581 tgagagctgg atgtagtagt cgttgaaccc gtacgtgtcg aggatctcgc ggatgagatc
     2641 gagcgcgcgg ccgaactcct cgccgatctg ctcctccgtg cagaagatgt ggcagtcgtc
     2701 ctgtgtcagc gcgcgaacgc gtgtcaggcc gctcagctcg ccgctcttct cgtaccggta
     2761 aagtgtggcg aactcagcgt agcgtagcgg aagctctcga tacgagtgca gctgctggtt
     2821 gtagagcgtc atgtgcgacg ggcagttcat cggcttcagg cgcatgcgaa cgccttcatc
     2881 ctccatgacc gggaacatga catccgagta gtgctcgagg tggcccgaac gcgcgaacag
     2941 ctcctccttg acgagatggc ctgtccagac gtgctgatag ccgtagcgtg tctgcacatc
     3001 tcggacgtat gactccatca ggtagcggac catctcgcct ttcgggaaga agatcgggat
     3061 gccctgaccg atgtcctcag agagcgtgaa cagcccaagc tcccggccaa cgcggcgatg
     3121 gtcgcgtttg cgggcttcct caagctgatt gaggtaggcg tcgaggtcgt ccttcgatgg
     3181 ccaggccgtg ccgtagatgc gctgcagcat cggtcgcttc tcgtcaccgc gccagtaggc
     3241 gccggcgatg ttgagcagct tgaacgcctt gatgttcgat gtgtcgcgga cgtgcgggcc
     3301 acgacagagg tcgaggaacg ggccgttctg gtaggtgctg ataacctcgt cggccggcag
     3361 attctcgatc agctccagct tatacggctg gtcggcgaag atcgaaagtg cttcgtcacg
     3421 cgagacctcg cggcgctcga acacctcgcg cgccttgatg tgcgtgcgca tgtttgcctc
     3481 gatctgttcg aggtcttcgg gcgaaagcga gcgcggcagg tcgaagtcat agtagaagcc
     3541 gttctcgatc gccggaccga tcgcgagctt ggcgtccggg aagagttcga gaaccgcttc
     3601 ggccataacg tgggcggctg agtggcgcat cctggcgaga tcgaactcag cctcgttctc
     3661 gggctggacc tgcatttcgt cgggcgcggc tgcctggcta accattagtg agctcctctg
     3721 gatgattcgt tgccaatagg tcgtcgtagg atcggtaggc aactggtagt tgctgcgccg
     3781 cgactgtacg tgcctggcat ccgaccctcc ttcccgtcga ttcctctcgc agtcgccgca
     3841 aatgaaagag cgcctgccgc cacaagggac ggcaggcgct gtcgcggcct ggcgtggttc
     3901 caccctcctt ccggcgcaga tcgcgccgct cagggttgcc cgctaacggg ggcaaggcgg
     3961 agacggctag tgacgcattg gcgcgttcgc cgctcggctc gcgggtggtt ttcccgaagg
     4021 tcgtgctcgc ggggcttcca gtcgctggcc ctacgtctct ggtgcacgag gattcggtac
     4081 tcgtcccggt cttcgccatc atgcgggaat ctggtgggcg gtactggact cgaaccagtg
     4141 acctctacga tgtcaacgta gcgctctaac cagctgagct aaccgcccgc gtcgaagatg
     4201 ataccaccgg gatggtggtg gtggcaattg gttttgcggg tcgcgtgccg cgtcccacgt
     4261 cccgttctac cgctgcgaag cttcgtagcg gaggcgttcg atgagtcggt cagataggtg
     4321 gatggcgatg tagatgggtt ggtagacgcc ctcggcgacc tgctgggcgc gcggcgcgtc
     4381 ggtgtcggtc aggcgacggc ggcggcgggt tgcttcgtcg aactgctcga cggccgtgtt
     4441 gatccagtcg atctcatcct cgcgcaggcg ttcgaggtgg gcatcggaaa gcgccagcag
     4501 cgatggcgag atgtcgctgg cggtggctga gaaacgtgac caggcgggtt catcgatatc
     4561 ttcgaagagg ggcttgacga ccgatttcca gcggtagcgc tggagtagcc atgtggcgta
     4621 gcgcatgtca tgcacggcgt tggttgtcat ctcgtcgcca ctctcgtact tcggtgggag
     4681 tggttgggtg cggcgatcgc gccagaggtc gcgccattcg ggccggagcc gggcggcgga
     4741 ttcgtagagt gattcaaggc tactgaagag gtcacgatag gcatccgcac gtgtgggccg
     4801 gttgcggtcg cggcggaaga acagcgtcgc ctgcgggacg acgaatgcgg caatcctgag
     4861 tgcggtccag tgtccggcaa cgatccattt catcgcgttg tcgactcctt tcgcgccggg
     4921 tgagccagcg actcgatggc gagcaggagc agggcactgg tgggccggtc cgccggcgat
     4981 cggctgatgt aacgatagcg aacgattccg tcggcgtcga taatgaagac ggccggccgg
     5041 gcgatgacgt gatcgacgct gtcgtcacga tcgacgagtc cgagttgctc gatgaacgcc
     5101 gccgagggat cggacaggaa gtcgatcgcg ccgcccgtgt ccaccgtcat gtcgtttgcg
     5161 ccgacgaagt catcggcgct gatcgcgatg atgctgaagt ttagcgagcg gaacgagtcg
     5221 tgctgcctgg cgaggtcggc gaactgtcca cgacagtggc tgcaccagtg tccacggtag
     5281 aagatgatga gtgcggagcg agggtcgatc gcgactggac gatcccggca gctacgcagt
     5341 tcgaaaggag ggaacgaatc tccgaccgcg acggcggggc tgaggaacga ggtgttcaat
     5401 gcccaagcag cctgacggat tcgatgcgcg gctgccagac gaggtgccaa ctcccgaaac
     5461 gtgagaggag tgctaacgcc tcttcgcgaa acggtcgcag cggcgcatgc cgaaccttcc
     5521 agcggccgtt gacctgttcg acgacgagct tcgagtcgga cagcacggcg acccgcatag
     5581 ttgccggatc gcgcgcgtcg cgttccagtt cgcgcaggat cgtgcggagg ccatggagca
     5641 tcgtctgata ctcagcttcg ttgttggtcg tctggcccgg gtagtcgagg cgaaccggcc
     5701 tttccgtctc gatgacgccg gtatagacga agctgccgta gcccttgccg ggatttccca
     5761 gcgatccgcc gtcgaagacg agcgtcacat cggcgtcggg ggcggatcgt gtgggcggcg
     5821 tcgggcggcg tgaaacccca gtcattcgat tcctctcacg gacaacaacg agtcccgcca
     5881 gatcggcggg actcgttctc gagtgtatca ggcgtttgct tcctggcggg ctcggtgggc
     5941 ggcaataatc gaatcagtca ggtgggacgg cacctgctgg tagtggctga actcggtcgt
     6001 gaacgttcca cgcccctgcg tcagcgagcg caggtcggtc gcgtagcgct ggacctcgga
     6061 ggcagggacg gtcgcctcga tggtcgtata gccatcgccg gacggcgtca tgccgctgac
     6121 atgggcgcgc ttgacggtca ggtcgcccat gatgtcgccg gtgtaggcat ccggtacggt
     6181 caccgagacg ttgagtaccg gctcgaggat gacggggttg gcggactggg ccgccttctt
     6241 gaacgcctgc gacgcggcca gcttgaaggc catctctgac gagtcgacgt tatggtacga
     6301 accgtccgtc agcgtcacct tgacgttgac gaccgggaac ccggccagga tgccctcctc
     6361 catcgcctcg cggaggccct tctcgacggc cgggatgtac tgacgcggga cagagccgcc
     6421 gaagatcttc tcctcgaact ggaactcgcc ctcggccggc tcgacgtcga tgaagacgtg
     6481 cccatactgc ccgtggccgc cggtctgctt cttgtgcttg tactcgatgt gctggacggc
     6541 cttctggatc gtctcgcggt agggtacaac cggcaggccg atatcgacgt tgacgccgaa
     6601 cttgcgcgcc atccgctcca tcgcgatctg gacgtgtgac tcgccgaggc cgctgacgac
     6661 cgtctcgcca gtggccgcgt cgcggccgat gtggagcgtt gggtcctcgt cggcgacgcg
     6721 ctggagggcg gtgcccatct tgtcgaggtc ggacttggtc ttcggtgcga cagcggccgt
     6781 gaaaagcggc tgcgggaact cgatgccgtc gatgagcacc ttgtgttgcg ggtcggccag
     6841 cgtgtcgccg gtggttgtct cgaccagctt cgcgacggcg ccgatgtcac cggcgcaaac
     6901 ctcgttgacg gcgatctgtt ccttgccgcg caggagatag agctgaccgg cgcgctcgtc
     6961 cttgttgcgg gtgacattcc agatgtgcga gtcggacttg agcgtgccgg agtagacgcg
     7021 gaagtaagtc agcttgccga cgaacgggtc ggcgatcgtc ttccagatga aggcggcgat
     7081 cggtccgttc ggatccgggg tgagcgtcac ctcgccgcct tcaggcgtct tcgccgcagt
     7141 cggaccgggt gcgggcgcga ggtcgatgat cgcatcgagg atgaccgagg tgccgcggtt
     7201 ggaggatgcc gcgccggaga ggaccgggac gatctcgccg gaggcgattc cggcgcggag
     7261 gccggcgcga agctcgtcag ctgagatctc gtcgtcttcc agatagcgga gcatcaactc
     7321 ttcgtcgttc tcggcgattt tctcgaccag ctggaggcgg taggtttcga cctcatcgag
     7381 catctcagcc ggaacgtcgg cttcggtgac ggtgccatcg cggctctcgg tgaacatcca
     7441 cgcgcgcatc ccgaggagat ctacgacgcc gcgcagcgcg gattcgctgc caatcgggat
     7501 gtagagcgga acgactttct ggccgaaggc cgcctgggcc gaggcgaggc tagcggcgaa
     7561 gttggcgttc tcgcggtcca tcttgttgat gatgacgatc gccggcaggt ttcgttcggc
     7621 ggcgaagcgc caggcgtact cggagccgac ttcgacgccg gcagacgcgt ccagcaggat
     7681 gacggctgtg tcaacgacac ggatggcagc cttgacctcg ccgacgaaat cggcatagcc
     7741 cggcgcgtcg atgatgttga tcttgtggtc tttccactcg acgggggcgg gtgtcgcgct
     7801 gatcgagatc gtgcgcttga tctcgtcggg atcccagtct gagacggtgt tgccttcttc
     7861 gacgcgcccg aggcgactga ttgccttacc tgtgaagagc atcgcttcgg tcaatgacgt
     7921 ctttccggcg ccaccatgcg agaacaaccc aatgttgcgg atgctttcga cggggtatgt
     7981 cttcacaggc cacctcccgt agaccgaact gaaacagggt gcgacgatga gtctggagcg
     8041 tccgagcatc gggctccctt gtcatggccg cgtcgtttca ggtcggaatc tcattgaatc
     8101 atcggtgttc cggtattcga tttcgaccgg atccgtcagg caccacctta ttatagcctt
     8161 cacggcagca caacacggca gcgtgaattt gaggaacgct acgggggaga gaaatggtcg
     8221 aacgggacgc gatgcggcag cgcatcaagg atctgctgca cgatctgtcg ggcctggatg
     8281 gggtttcagg tcacgagcat gccgtcgtgc agcgactggt ggagttgcta tcgccgcatg
     8341 ccgagacgac ggtcgatccg tacggcaacg tcttcgcccg ggtcaacgac gccggcagcg
     8401 gtccgtcgct gatggtggcg gctcattcgg acgagatcgg cgcactggtc aagtcgatcg
     8461 agccaaacgg gatgatccgc ttcgagcgcg ttggcggcgt gatcgagacg ctgctgatcg
     8521 gccggcatgt acgcatccgt gggttgcgcg ggatcgttgg ggtcaaggca ggccatgtcc
     8581 agtcggcgga cgagcgtggc cgcgcaccgg cgatccgcga actgtacatc gacatggggt
     8641 tcgactctgc tgccgaggtt gaggcgctgg gaatccgcgt cggcgacgcg atcgcttacg
     8701 acgagccgat ggaggaactg gcgaacccgg ttcgagtcag cggcaaggcg ctcgacaacc
     8761 gggtgtcatg cgcgctggtg gtgctgctcg ccgaacggct gcgcggtgtg acgctcggct
     8821 gcacgctgca cgtcgtcgta acggtccagg aagaagtggg tctgcgcggg gcgcgaatcg
     8881 cgacgcaccg gctcaacccc gacgcggcaa tcgtcgtcga cacggtgccg tcgggcggga
     8941 caccggatgt cgacttccac aaggatctgc ggatgcggat tggcggcggg ccggtgctgg
     9001 ggctggcgag ttccggtggt tcggccggcc atcacatcaa ccctgccatg cgcgatttcc
     9061 tgcgccggac ggcggcagat gccggaatca cattgcagga agcagtcttc tatggcggca
     9121 cgtccgacgc gtcagccgtc catctggtgc acgatgggat tccgaccggc gtcatcaata
     9181 tcgcccggcg gtactcacat tcgccggtcg agacgttgga cctgaacgac gctgtcgata
     9241 cactgctgct gctcgaagcg gtggcgcgcg cgttcaacgc cgggaccgac ttcggctttc
     9301 tggctgcgga gtggggatag ctgtctaacg cgcggattgc agatggcgac gccagacgag
     9361 gcgcagtcca tcgatcgtga gatccggcgc aaacgttgag attcgcgggc agcggtcctt
     9421 gaagacgccg ccgaagccgc cggtcgagac gacggtcggc tgctcgccca actcctcatc
     9481 aatttgcgcg atcaggccgt tgatgaggcc gacatacccc aacatgatgc cggcctgcat
     9541 ggcggcgacc gtgttgtggc cgatcaccga ggccgggaag acgagatcga cgctggtgag
     9601 cttggcggct ttgccgacca gtgcgtcgtg ggccgagcgt aggtcgggcg cgatcgcgcc
     9661 accgatgaag gcgccacgct cgttgacgat gttgagcgtc gttgccgtcc cgaagtcgag
     9721 gacaacgact gggccgccga agcgatcgaa tgcggcggcg gcgtcgacaa gccggtcggg
     9781 tccgacctcg gtcgggttgt cgatctcgac agtgatgccg aggtcggttg tcgggccgac
     9841 gatgaccggc tcgatccgga tccggtcgcg aatcatcgtc gtgagccaag gagtgacgac
     9901 ggggacgctg ctgctgacga cggcgccgtc gatcgcggtc aggtcgatcc cgtcggcgac
     9961 ggctagcgtg ttgaggacaa tccaccactc gtcgggcatg ctggtgcggt cggtgtggac
    10021 ccgccagcgg gagtggagct caccgtcgat gtcgatgccg aacttgacgt tggtgttccc
    10081 gatatcgacc agcagcagca tgactgtccc ttctacggtt ccggattcga tgccggaacg
    10141 attcaacgcg gatgaagatc gacagccgtc tccgccgggg ccactgcgaa cttgggttcg
    10201 agcccgcgtt cgatctcctc acggacatac gattcccgct cgcgggacca ggctgcacgc
    10261 aacgtccgtt tcgcatcctc gccgacgagt tgacgtaacg caccagcagc atgtccacgc
    10321 gccagcggct cgtcatggtt caagagcatt gcgatgagga tcggttcagc ccctggatcg
    10381 cggctgttgc cgagtgcgat ggcggcgttg cgcgccatcc cggcccgctt ggcgcgggtg
    10441 acaggcgtgc cgctataggt ggcgcgaaac tgctcctcgc tcatcgtggc caggaattcg
    10501 agcgagggaa acgcgttgtc aaccgatcga ggtgcgaagt ctgcgtcatc aacatggcgc
    10561 gcggcgctgg tgtaaggaca gacatcctgg caaatgtcac agccgaagac gaagttgccc
    10621 atcaaaggcc ggagatggtg cggaataacg ccacggtgct caatcgtcag gtaggagatg
    10681 cagcgcgggg cgtcgatgcg gtacggctcg acgatggcgc cggtcggaca gcggtcgatg
    10741 cagatctggc accggccgca gtccttcgcc atcggcacgt ccgacgggat cgtgatatcg
    10801 agcatgatct cgccgagcat gacccacgat ccgtgtccgg gaacgatgat catcgagttc
    10861 ttgccgtacc agccggcgcc cgagcgagcg gcgacagcgc ggtcgacgac ccgggcggta
    10921 tcgacgagca cacgcgcttc gaccgtgcgg ccgacgcgct gctcaatggc cgcgacgagg
    10981 tcgcgcatac gccgtttcag cgtgttgtga tagtcacggc cccaggcata gcgggcgatc
    11041 cggccgcgca ggacgccgtc gcccggtggg ttcgcctggc cgctccagaa cgggacgccg
    11101 accgagacaa tcgtccggac cgagtcgtgg agcgttcggg ggtcggcgga ttggcgcacg
    11161 cgctcgaccg tgaaccagtc catgccatgc atgtgcccgc gttcaacatg gtcgacgacg
    11221 taggctccga catccctgaa tggcgcgccg tccgtgacac cggtgatggt caggttggcg
    11281 gcggtcgcca gcgtggtgag gtctgtccga gtgagatcga aggtcgtcat ccgggcaact
    11341 cgtggccgct gagtaccctc tcgacctcgg cgcgggttgg cagaccggcg cgggcaccaa
    11401 gggcgcgaca ggcgagacct ccaagggcgt tggcgcgccg caggatgtcc gcgtcatcca
    11461 gccgatcgag cagcccccag aggcagccgg ctgcgaaggc gtcgccagcc ccggtcgtgt
    11521 cgatgacctc gatggcaacg gccggcgcca gctcgacgcc ggactcgcgg atggcgatcg
    11581 cgccgtacgg accgcgactg aggaagacga cctggcaggc gctggcgacc aggtcgcgct
    11641 ggacctggtc tatcgccgcg tccagcgatt ccgcgcccag caacgttcgc agctcgcgtt
    11701 cgttgccggt gacggcatcg aaccggagtg catgctgcca gcctgcccca ggcggcatct
    11761 cgaccagata cgtcatcgtg ccgaacagct tcgttcgggg gctgcgatgg gccggcaggt
    11821 cgaggaagaa cgagcgcagg cggggatcgt cgatgtcgat gtggacccac tcgtgttcca
    11881 gcatctcgtc gaccggcaac gggtcaccca tcagcggttt gacaccctgg atccagtaga
    11941 tcgtgcggtc gcgctggctg ggcggacctg agacgatgat gatgccggtg tcggaacgag
    12001 cgccttcgcg gatcgcgatc gcgccggtgt cacagcctgc atcgcgcagg gagtcgatca
    12061 acgcggctcc ctccggatcg tcgccaacga cagagacgag caatacattg acgccgacct
    12121 tcgcgagcgc gtggcaggtg ttgccggttg tcccgccggc ctgttcgagt tgatggcgga
    12181 cgacggcgta ctcgccgggg ccagggtagt cgtcggtgac gacaaatcgg tcccacgaac
    12241 agagtccaat ggcggccaca ccgcgacgca tcggctggat cctcccttcg cgcgccaacc
    12301 cggacgcgtc agcgtcgaac gatagcatag cgggcggcca gggcaggcgg ctggcgcggg
    12361 tacaatcagc gacggcacgc agggggagcg atggctgacg cgtcgttgat cgtggtcgaa
    12421 gatgaaccgg cgttggctga cgctctgcgc ttcaatctgg agcgacaggg ctacgacgtc
    12481 agcgtcatca ccgacggcca cgccgccgtg cgcgagatcg ggcgctcaca accggatctg
    12541 gtgattcttg acgtgatgtt gccggggctg gacggctttg aggtctgtcg tcgggtgcgg
    12601 ctgcactcga cggtgccgat tctgatgctg acagcgcgtg gcgaggaagt ggacagggtg
    12661 ctcgggctgg agatcggcgc tgacgactac ctgaccaagc cattcagcct gcgtgagcta
    12721 caggcacgag tgcgggcgtt gctgcgccgt tcgtcggcac agcagccgcg agaacgcagc
    12781 gaggtccggc aggtggggaa tctacgtatc tcggacgccg agcggcgtgc ctggctcgat
    12841 gaacaggagc tacagctgcg accacgcgag ttcgatttgc tgtcgttcct cgtccgcaat
    12901 cagtcgctgg cgctcaccag gcagcagatt ctggacggcg cctgggagcg tgacttcgcc
    12961 ggtgacgaac gaacggtcga cgtccatgtt aaggcgcttc gagacctgat agaggatgac
    13021 cctcgccagc ctgtgcggat cgtgaccgtt cggggcgtcg gctatcgatt cgaagggtaa
    13081 ccggatgcgt ggtctgtcta tccagttcat gctgccggac ttcgtcgttc tggcggcgct
    13141 agtcatcggc tggcttgccg gttcgacagg ctggggcgtc ggcgttggcg gcagcctggc
    13201 gctcgtcgtc gcgatcgggc tggcacggcg tgctgaacgc cggctggagc atctggagcg
    13261 gatggttcgg ccggatgccg ggctggatct cgcggatgcg gcgacgatcg acgtccacga
    13321 tcagatcgac cacgccattc gaacggtcgc gcagcaggcg ctgcaccagc gcaccgcgct
    13381 ggacgcaatg gagttgaacc tcacgcagca agcgcaggtg ctcgaccaga tgaacgatgg
    13441 gttgatgcgc gttgcgctcg atggccgcgt gacctacgcg aacgtcgcgg ccggatcgct
    13501 ctttggtggc cgcaacccga ccggccgcac gttcatgagc gtgacccatg accatgagtt
    13561 gaaccgggcc gtacgagctt gtctggagtc gggcaatgtc cagcaccaca cgctcgaaat
    13621 acccggtgaa gggcggctcg tcgacgctgt cgcgattcga ctgagcgagc gaccgatcga
    13681 ggtactggtg atgctacgcg acatcaccga ggttgcgcgg ctgcagaacc tgcgacgcga
    13741 cttcgtttcg aacgtatcgc atgagttgcg gacaccactc tcgacgatca agatcctgac
    13801 cgagacgttg ctggacatcc aggccggcga tagcgaggcg agcggctttc tacaacagat
    13861 cgatggcgag gtcgattcga tgacagcgct cgtcaaggat ctgctcgatc tcacgcggct
    13921 ggagacgacc ggttcggcgc tggccttgcg ggatgtcgac gctgcgctgc tcgtaactga
    13981 cgccgtcgac cgcatgcgcc cgttggcgcg gcgtcacgat gtcacgctcg atgtcgatat
    14041 cggcgatggc gccggtcatc tggcagctga tgagcggcgg ttactgcagg cgatgatcaa
    14101 cctgatctcc aacgcgatcg tccacaccga gtcctgcggg acggtgactg ccggagcgcg
    14161 acgggagccg ggcggagtgc gctttttcgt gcgcgacacc ggctggggca tccctcccga
    14221 cgacctgccg cgtatctggg agcggttctt caaggccgac cgtgcccgaa ccggacctgg
    14281 gacgggactc ggtctggcga tcgtcaagca catcgttcag gcacatgggg gaagcgtcgc
    14341 cgcgaccagc gagctcggtc acggctcgga gttttcgttc gagattcccg acaacctgga
    14401 gccggtggcg aagctaccac cggcggtaga gcttcctgtc gcaccgaact agcgcgtccc
    14461 ctgtcgtcct gagccgcagc gaaggacctc tctgcttgaa cactcatgtg gctaaaacgg
    14521 ggaggtcctt cgctgcgg
//
LOCUS       DASUGJ010000216         5688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_585290, whole genome shotgun sequence.
ACCESSION   DASUGJ010000216 DASUGJ010000000
VERSION     DASUGJ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585290"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..794)
                     /locus_tag="VFV93_04785"
     CDS             complement(42..794)
                     /locus_tag="VFV93_04785"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX5164691.1"
                     /translation="MSPWLIVNADDFGRSPGINRGIVEAHQRGIVSSTTLMVNLPAST
                     AAAALAIITPDLGIGLHLNYCYGPPLASGVDSLLGPDGLLDRDLHRLRQRATVDDLTR
                     EARAQLHLFRELLGRDPTHLDSHQHVHSWPVAIGPVVALARELGVPIRACTAEHRATL
                     RMFGVRCPDTFIIDFYGTDKIDTGTLAERLSTLPPGVTELMCHPGYVDAEIADSSYRT
                     EREEELATLCSPAIGRLVSELQIELVTFASLG"
     gene            complement(796..1206)
                     /locus_tag="VFV93_04790"
     CDS             complement(796..1206)
                     /locus_tag="VFV93_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5164692.1"
                     /translation="MTERPMILVVEDDDAVAMMIQDYLSGHDFRTARAADGREAVDMV
                     REQHPDLIVMDLMMPRLTGGEAATALRRDPLTEKIPIVAISAVADVTSIAEMLPLDEV
                     LPKPFDLDDLVAAIERLLPVDTGQPLDQAEPASP"
     gene            complement(1392..2501)
                     /locus_tag="VFV93_04795"
     CDS             complement(1392..2501)
                     /locus_tag="VFV93_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX5164693.1"
                     /translation="MGVARAIQVGMPVEQLETPVLTIDIDAMQRNLDKMMGILAGSKV
                     RLRPHLKTAKSPALAHMMVRAGAVGVCAAKLSEAEVLANAGITDILLTSEVAGAGKIE
                     RLVALASRLPDFKAVVDNGPIVEQIARLARERGVTVKLLIELDVHTGRSGVVTPEGVL
                     ALANLIRETDGVEFVGVHGYAGHAQVRPEAERIERNGPAMEILSETVELLKEHNHDVR
                     YVTGGGTGTCAMDVENGVLNEVQAGSFLLMDVLYRNAGVPFENALRCQTTIISRPTPE
                     RAVCDAGGKTMSHDGGPPEVENHPGLRYLRGSDEHGSMAVDPAELEGELKVGDIVSLL
                     PSHICTTINLHDVLVGVRGGVVEAIWPVEGRGRVW"
     gene            complement(2633..2959)
                     /locus_tag="VFV93_04800"
     CDS             complement(2633..2959)
                     /locus_tag="VFV93_04800"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5164694.1"
                     /translation="MDAFSLDDLARTTRQSGERFHEFLRVDSLSCGMYILPAGGEDDQ
                     VPHREDEIYVVSKGTGRIVVDGETQPVGPGSVIFVAANVEHRFIDFSEDLEILVLFAP
                     AYSGRG"
     gene            3199..4176
                     /locus_tag="VFV93_04805"
     CDS             3199..4176
                     /locus_tag="VFV93_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HEX5164695.1"
                     /translation="MLETIVKRLTVAAAAGMFLVLIQGTLVTNTGSADGCGNSWPLCH
                     GKFVPQYTVATAIEYSHRLVVSVESALIIAASLGVLWLWRQRKEFRIYVPFMIFFLFL
                     QAGLGAASVMWPQAEEIKALHFGVSLVAFASTLLTAVLAYEQHGRDKLRDRPIPRWLI
                     WTVWGLAGYTYLVVYLGAYTRHTDSSLACTDWPLCNGSAFPGFSGPVGIVFSHRIAAL
                     VLVIAMAALLSYTVRMRDYRPDLYWGSIIALVLVIAQAIGGAAVVWSRMDVFATLSHG
                     TIVSLFFGTLAYLCIHVLPRPESARLTAPAPERGERFSPAPSPAGSTGD"
     gene            complement(4190..4729)
                     /locus_tag="VFV93_04810"
     CDS             complement(4190..4729)
                     /locus_tag="VFV93_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164696.1"
                     /translation="MIDRDELQRDPYDHDYQNVDEETIPDRRDMIRIVFWIVSALIAI
                     GMVAWPVIRVIDWGDDDSDGPSAASQARAFVAERFASDALVRFSASDAARWAVPSVRR
                     DIDAIVDDLRQRPATDFQGAAVTVARVSCPDQTEPDSECFHAWIRQPGAKDLLRLLLV
                     VSIVNGDARVTEIERINVV"
     gene            complement(4754..>5688)
                     /locus_tag="VFV93_04815"
     CDS             complement(4754..>5688)
                     /locus_tag="VFV93_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5164697.1"
                     /translation="AIRQVLDANPDVVSYATSMNFNAVVPGMGIEHAFDLRALGADVQ
                     HSGYPLIEGRMPDGPGEAIVGRVLFDQLGLHVGDQVSLEAIRGPFGEQQPVPVTLTIV
                     GSYVDPEEDGEVMLFTAETAEQLLPGIQPDTYEVMMQNDSGWDELLVDVQAATNFGVH
                     VDLRERGTPGEITTIRGIMYGLCSVLLIIGVANMLTTLLLNVRERVRDFGIFKTLGMS
                     PRQVVSSVASGTGLLTLLAIVVGIPLGLVLFRILFVVVGENMAGADPKLYASPSWAGI
                     VLIVVGAIIFAGLCTILPARRAANVRAVEVLRYE"
BASE COUNT          985 a         1891 c         1808 g         1004 t
ORIGIN      
        1 gggcgatttt gcgaaatcgc ccctaccgga ttgggattga ttcatccaag tgaggcgaac
       61 gtcaccaact cgatctgtaa ttcactgacc agcctgccga tagccggtga gcagagcgtc
      121 gccaattcct cttcgcgctc cgttcggtag gagctgtcgg cgatttcggc gtccacgtag
      181 ccggggtggc acatcagctc ggtcacgcca ggcggcagcg tcgagagtcg ttcggccaac
      241 gtgccggtgt cgatcttgtc ggtcccgtag aagtcgatga tgaacgtgtc cgggcaacga
      301 accccgaaca tccgcagcgt ggcgcggtgc tcggccgtgc aggcgcggat cgggacgcca
      361 agctcccgcg ccagcgcgac gaccggtccg atcgcgaccg gccaggagtg gacgtgctgg
      421 tggctgtcga gatgggtcgg gtcgcggccc agcagctcgc ggaaaaggtg gagctgcgca
      481 cgcgcttcac gagtgagatc gtcgaccgtc gcgcgctggc gcaggcggtg aaggtcgcgg
      541 tccagcaagc cgtccgggcc gaggagcgaa tcgacgccgc ttgccagcgg tggaccgtag
      601 cagtagttca gatggagacc gattccgagg tcaggagtga tgatggccag cgcggcggca
      661 gcggttgagg cgggcaggtt gaccatcagc gtcgtgctgc tgacgatccc gcgctggtgg
      721 gcctcgacga tgccacgatt gatgccgggc gaacggccaa agtcgtccgc gttgacgatc
      781 agccagggcg acaccttacg gcgaggccgg ctcggcctga tcgagtggct gtccggtatc
      841 aaccggcagc aagcgctcaa tggcagctac caggtcatcg aggtcgaacg gcttcggcaa
      901 cacttcgtcc agcgggagca tctcggcgat cgaggtgacg tcagcgacgg ctgaaatcgc
      961 gacgatcgga atcttctccg tcagcgggtc acggcgcaat gcggtggccg cctcgccgcc
     1021 cgtcagccgc ggcatcatca gatccatcac gatcagatcg ggatgctgct cccggaccat
     1081 gtcgaccgcc tcgcggccgt ccgccgcacg ggccgtccgg aagtcgtggc cgctgagata
     1141 gtcctggatc atcatcgcga ccgcgtcgtc atcctcaacg accagaatca tcggccgttc
     1201 ggtcattggt ctgtccttcg cttgaggtca ggcaggtatc ccgtccatca gcaacgagga
     1261 tgccagccgc tggccgtgct gtggatgacg ttaccagatg gacgggtgtg actcaatgtg
     1321 accgatgtcg tggttctccc ggcatcagat gacccgaatg tcctcgcctg accgccccga
     1381 attcggacgg cttaccagac gcgcccgcga ccctcgaccg gccagatcgc ctcgacgacc
     1441 ccgccgcgaa cgccgacgag tacatcgtgc aggttgatgg tggtgcaaat gtggctgggc
     1501 agtagcgaaa cgatgtcgcc gaccttcaac tcgccttcga gttcggcggg atcgacagcc
     1561 atcgagccgt gctcgtccga gccacgcagg tagcgcaggc cggggtggtt ctcgacctcc
     1621 ggcgggccgc cgtcgtggga catcgtcttg ccgccggcgt cgcagacggc ccgctcgggc
     1681 gtcggccggc tgatgatcgt cgtctggcag cgtagcgcgt tctcgaacgg cacgccggcg
     1741 ttgcgataca gtacgtccat cagcaggaac gacccggcct ggacctcgtt gaggacgccg
     1801 ttctcaacat ccatcgcgca ggtgccggtg ccgccgccag tgacgtagcg tacgtcgtgg
     1861 ttgtgctcct tgagcagttc aaccgtctcg gagaggatct ccatcgccgg cccgttgcgt
     1921 tcgatccgct ccgcctccgg ccgcacctgg gcatgcccgg cgtagccgtg gacaccgacg
     1981 aactctacac cgtcggtctc gcggatcagg ttggcgagcg ccagcacccc ctcaggcgtg
     2041 acgacgccgc tccggccggt gtggacatcc agctcgatca gcagcttgac ggtgactcca
     2101 cgctcgcggg ccagtcgcgc aatctgctcg acgatcggcc cgttatcgac gacggccttg
     2161 aaatccggca gccgggaggc caaagcgacc agccgctcga tcttgccggc gccggccacc
     2221 tcgctcgtca gcaggatgtc ggtgataccg gcgttcgcca gcacctcagc ctcggagagc
     2281 ttggcggcgc agacgccgac cgccccggcc cgcaccatca tgtgcgccag cgccggcgac
     2341 ttggccgtct tcagatgcgg ccggagccga actttcgagc cggccagaat acccatcatc
     2401 ttgtccagat tgcgctgcat cgcgtcgatg tcgatcgtca ggactggagt ctccagctgc
     2461 tcgaccggca tcccgacctg tatcgcgcgt gctacgccca tctcaaccta cctccactca
     2521 caaacaaccc ctcaccgcgt cgcgcacatg ctaccagcga atcgccgatt ccgcgcgtgt
     2581 cgtacccagg cttgtctgcc gtcgatgaga tattgattgg tttggctttc tgtcagcccc
     2641 gcccggaata ggccggggcg aacagcacca ggatttcgag atcctcgctg aagtcgatga
     2701 agcgatgctc gacgttggcg gcgacgaaga tgacgctgcc ggggccgacc ggttgggtct
     2761 cgccgtcgac gacgatccgg ccggtccctt tcgagacaac gtagatctcg tcctcgcggt
     2821 gcggcacctg gtcgtcctcg ccaccggccg gcaggatgta cataccgcaa ctcagggaat
     2881 cgacgcgcag gaactcgtgg aagcgctcgc cggattggcg cgtggtccgg gccagatcat
     2941 ccaggctgaa ggcatccatc agatgacgtc ctctctgtca ggtgactgtg acggggtcta
     3001 cggtattcga ttcagcttca caccgacaag caccacctcg aacgactgca cgccgctggc
     3061 tggtccgcga aagtcgccgg gcaagcggac tcgtcgtatc cttagcgacg tgcaggcgat
     3121 ggcaggggac gcatcgccgc agcgactact cggcgccggc agcctgattg ggcgcggcgc
     3181 gcgtgcggga ctggaccaat gctggaaacc atcgtcaagc ggctgacggt tgccgccgca
     3241 gccggcatgt ttctcgtgct gattcagggc acgctcgtca ccaacaccgg ctcggccgac
     3301 ggatgcggca actcgtggcc gctctgccac ggcaagttcg tcccgcagta cacggtcgcg
     3361 acggcaattg agtattccca ccggctggtc gtcagcgtcg agagcgcgtt gatcatcgcg
     3421 gcctcgctgg gtgtcctttg gctctggcgg cagcgcaagg aattccgcat ctacgtcccc
     3481 ttcatgatct tcttcctgtt cctgcaagcc ggcctcggcg ctgcgtcggt catgtggccg
     3541 caggccgaag agatcaaggc gctgcacttc ggtgtctccc tggtcgcctt cgccagtacg
     3601 ttgctgacgg cggtcctcgc ctacgaacag cacggccgcg acaaattgcg cgaccgacca
     3661 atcccacgct ggctgatctg gaccgtctgg ggactggccg gctacaccta cctggtcgtc
     3721 tacctcggcg cctacacgcg ccacaccgac tccagcctgg cctgcaccga ctggcccctc
     3781 tgcaatggca gcgccttccc cggcttctcc ggcccggtcg ggatcgtctt cagtcaccgg
     3841 atcgccgcac tcgtgctggt catcgcgatg gcggcgttgc tttcatacac ggtcaggatg
     3901 cgcgactacc ggcccgacct ctactggggc agcatcatcg cgctcgtgct ggtgatcgcg
     3961 caggcgatcg gcggcgcggc tgtcgtctgg tcgcggatgg acgtcttcgc gaccctctcg
     4021 cacggcacga tcgtctcgct cttcttcggc acgcttgcgt atctgtgcat ccacgtcctg
     4081 ccacgtcccg agagcgccag gctcaccgcc ccggcgcctg aacgcggcga acgcttcagc
     4141 ccggcaccat cgccggccgg ttcgacgggc gactagaatc accccgtgat cagacgacgt
     4201 tgatccgctc gatttccgtg acccgcgcat cgccgttgac gatgctgacg acgagcaata
     4261 agcggagtag atccttcgcg cccggctggc ggatccaggc gtggaagcat tcgctgtccg
     4321 gctcggtctg gtccgggcag gacacgcggg cgacggtgac ggcagcgccc tgaaagtcgg
     4381 ttgctgggcg ctggcgcagg tcgtcgacga tcgcgtcgat gtcgcgccgg acagacggga
     4441 cggcccagcg cgcagcgtcg ctggccgaga agcgcaccag cgcgtcggag gcgaaacgtt
     4501 cggcgacaaa cgcgcgtgcc tgactggcgg ccgagggacc gtcgctgtca tcatcgcccc
     4561 agtcgatgac gcggatcacc ggccaggcga ccatgccgat agcgatcaat gccgagacga
     4621 tccagaacac gatgcgaatc atgtcgcgcc ggtctggaat cgtctcctca tcgacgttct
     4681 ggtagtcatg gtcgtatgga tcgcgttgaa gctcgtctcg atcaatcaca gcgtcattct
     4741 acgcggcgaa gccctattcg tagcgcaaca cctcgaccgc ccgaacgttg gcggcgcgcc
     4801 gggccggcag gatcgtacac aacccggcga agatgatcgc tccaaccacg atcaacacga
     4861 tcccggccca ggacggggac gcgtacagct tcgggtcggc gccggccatg ttctcgccaa
     4921 cgacgacgaa caggatccgg aagaggacga gccccaacgg aataccgacg acgatggcca
     4981 gcaacgtcag caggccggtt cccgatgcga cactgctcac cacctgacgc ggcgacatcc
     5041 ccagcgtctt gaagatgccg aagtcgcgga cgcgttcgcg aacgttgagc agcaacgtcg
     5101 tcagcatgtt ggcgacaccg atgatgagca ggacgctgca gagtccgtac atgatgccgc
     5161 gaatggtcgt gatctcgccc ggtgtcccac gctcgcgcag atcgacgtgc acgccgaagt
     5221 tcgtggccgc ctgaacatcg accagcagct cgtcccagcc ggaatcgttc tgcatcatga
     5281 cctcgtaggt gtctggctgg attcccggga gcagttgctc ggccgtctcg gcagtaaaca
     5341 gcatcacctc gccgtcttct tccggatcga cgtagctgcc gacgatcgtc agcgtgaccg
     5401 gcactggttg ttgttcgcca aacggtccac gaatcgcttc gagcgacacc tggtcgccga
     5461 cgtgcaggcc aagctggtcg aacagcaccc ggccgacgat cgcttcgccg gggccgtccg
     5521 gcatccgtcc ctcgatcagt gggtagccag aatgctgaac atcggcgccg agcgcccgca
     5581 gatcgaaggc atgctcgatt cccatgccgg ggacaacggc gttgaagttc atcgacgtgg
     5641 catacgacac gacgtcgggg ttcgcatcca gcacctggcg tatggcgg
//
LOCUS       DASUGJ010000217         3153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1141883, whole genome shotgun sequence.
ACCESSION   DASUGJ010000217 DASUGJ010000000
VERSION     DASUGJ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141883"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1100)
                     /locus_tag="VFV93_04820"
     CDS             complement(120..1100)
                     /locus_tag="VFV93_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5164698.1"
                     /translation="MQSTLPELLERLGGKRVLVVADLYLDRYIFGSPSRISREAPIMV
                     LDEERQEDHLGGGAAPALQLAALGYEVAVAGVVGDDRAGQQVVALLAEAGIDPGAVLI
                     DPTRPTTTKTRVVAEGFFLFPQQVVRIDRQERAPVGADVTQALAAAVNATSPDAILVS
                     DYRSGVVTGELVDAIREAQALHGLLTTVDSQGDLDKFAGFDLVKCNQAEAEAVIGHPL
                     GDRASRETALLALRERIGARCLVVTRGGEGAALAGIDGYAEAPASNRSEIFDVTGAGD
                     TVVAVMTAARLAGADPLLAASLAQAAAGVVVRKWGNQQATREEIAAEIGR"
     gene            complement(1111..1500)
                     /locus_tag="VFV93_04825"
     CDS             complement(1111..1500)
                     /locus_tag="VFV93_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164699.1"
                     /translation="MIYQASRTGIAAGVVVGIVAATLAGIGWGLWPSWEFYWVMILGF
                     GVSESISWATKYKRGTELMVVAMACVVLGICVSRGVMAWDSPVLTLDMLLNHSDDPFV
                     RMAFQLEFIPDVLFMAIPFVINYIRFK"
     gene            complement(1689..2480)
                     /locus_tag="VFV93_04830"
     CDS             complement(1689..2480)
                     /locus_tag="VFV93_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX5164700.1"
                     /translation="MTTNVPRQRQSILGAPVDPVNLDDALALIAGWLQPSEPPSLHHV
                     VTVNPEFVIAARRDPAFANVLRAAALATADGVGISLAGRLLGVPIGERVTGVDLVEGL
                     AGLSAGDERCRLFLLGAGPGVAREAAERLRERFPGLRVAGTFSGSSHDAGFAEIDAQL
                     AGSGATVLLVAFGHPTQDLWIARQREALAAHGILISAGVGGSFDYLSGRIPRAPGLVR
                     RLGLEWLYRLVRQPWRWRRQLALPLFVLLVLRERLRTLRPTRTTL"
     gene            2541..>3153
                     /locus_tag="VFV93_04835"
     CDS             2541..>3153
                     /locus_tag="VFV93_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164701.1"
                     /translation="MTAFPPAPDAQLASRLLDVLVRHRQVPDQTGWRAEVAPGGSTDR
                     TFRILDPTGAPRLTARLARPGLTEHLRHEQRTLLELAGEATPSTTCASPRDVVLIEDD
                     GLAEQMLLVHAHMPGNPHPLGDVSSDARERLGSCLAWVHGHQREGYTIWPSLEGNVGT
                     RADLYHDRLATLRRYQSADQLTDAGTLLDRLTRADLPASAGWHE"
BASE COUNT          520 a         1104 c         1063 g          466 t
ORIGIN      
        1 ggtaatgcag ggcagatcgc cagaaatcgg ccatcctcat cctgctcaat cacgacagtg
       61 aactcaaatt ctctcacggc atgatcgcct ttctttcagg aggagtgtct cacaaccaat
      121 cagcgcccga tctccgccgc aatctcctcc cgcgtcgcct gctggttgcc ccatttgcgg
      181 acgacgacgc ctgcggcggc ctgggcgaga gaggcggcca ggagcgggtc ggcgccggcc
      241 aggcgggcgg cggtcatgac ggcgacgacc gtgtcaccgg cgccggtgac gtcaaagatc
      301 tccgagcggt tgctggcggg tgcctcagcg tagccgtcga tgccggccag cgcggcgccc
      361 tcgcctccgc gcgtgacgac caggcagcgg gcgccgatcc gttcgcggag tgccagcaga
      421 gctgtctcgc ggctggcgcg gtcgccgagc gggtggccga tgaccgcctc ggcctcggct
      481 tggttgcatt tcacgaggtc gaatccggcg aacttgtcca ggtcgccctg cgaatcgacc
      541 gttgtcagca ggccgtgcag tgcctgcgcc tcgcggatcg cgtcgaccag ttcgccagtc
      601 acgacgccgg agcggtagtc ggagacgagg atcgcgtcgg gactggtcgc gttgacggcg
      661 gcggccagcg cctgggtcac gtcggcgccg acgggcgcgc gttcctgccg gtcgatccgg
      721 accacctgct gggggaagag gaagaacccc tcagcgacga cacgcgtctt ggtcgtcgtt
      781 ggccgggtcg ggtcgatcag gactgcgccg gggtcgatgc cggcctcggc cagcagcgcg
      841 acgacctgtt gcccggcccg gtcatcgccg acgacgccgg cgacggcaac ctcgtagccg
      901 agcgcggcca attgcagggc cggggccgcg ccgccgccga gatggtcctc ttgtcgctcc
      961 tcgtcgagga ccatgatcgg cgcctcgcgg gagatgcggc tcgggctgcc gaagatgtag
     1021 cggtcgagat agagatcggc gacgacaagg acgcgcttgc cccccagccg ttcgagcagt
     1081 tcaggcaacg tcgactgcat cggccgcacc ctacttgaag cggatgtagt tgatgacgaa
     1141 cgggatcgcc atgaagagca cgtcggggat gaactcgagc tggaacgcca tccgcacgaa
     1201 cggatcgtcc gagtggttga gcagcatatc gagtgtcagg accgggctat cccaggccat
     1261 cacaccccgc gagacgcaga tgccgaggac gacgcaggcc atcgcgacga ccatcagctc
     1321 tgtcccgcgc ttgtacttcg tcgcccacga gatcgattcg gagacgccga agccgaggat
     1381 catgacccag tagaactccc agcttggcca gagcccccag ccgatcccgg ccaacgtcgc
     1441 ggcgacgatg ccaacgacga caccggccgc gattcccgtt cgacttgcct ggtagatcaa
     1501 gggctttggt cctcgcgcgc aatccggaca gcggtagccg atcggggttg cgaccatgca
     1561 gcgcgggcag atcggcttct cacagcgcga gcatcgcagg cgggttgaga cattcgggtg
     1621 gtacgtgcac ttcatatcga agtcgtcgga tgtcggcacg gtgatagctc agcccgccag
     1681 attcctcgtc acagcgtcgt tctcgtcggt cggagagtac gcagccgctc gcggaggacc
     1741 agtagcacaa acagcggcaa cgcaagctgc cggcgccagc gccacggctg cctgacgagc
     1801 cggtagagcc actccagccc cagccgtcgc accagccccg gcgcacgcgg gattcgcccc
     1861 gatagatagt caaatgaccc gccgacacca gcggaaatca gtatgccatg cgccgcaagt
     1921 gcttcccgct ggcgcgcgat ccagaggtcc tgcgttgggt ggccgaaggc gaccagcaac
     1981 actgtcgccc cgcttcctgc cagttgcgcg tcgatctccg cgaagccggc gtcgtggctc
     2041 gatcccgaga acgtcccggc cacccgcagg ccggggaagc gctcacgcag ccgctcggcc
     2101 gcctcgcggg cgacgcccgg cccggccccc agcaggaaca gtcggcaacg ctcgtccccc
     2161 gcgctgaggc cggccagccc ctcgacgagg tcaacgccgg tcacccgctc gccgatcggg
     2221 acgccgagca gtcgtcctgc caggctgatc ccgacgccgt cggccgtcgc cagcgcggct
     2281 gcccgcagga cgttggcgaa ggccggatcg cgacgggcgg cgatcacgaa ctcggggttg
     2341 acggtcacga cgtggtggag gctgggcggt tcagagggtt ggagccagcc ggcgatcagg
     2401 gccagcgcgt cgtccaggtt gaccgggtca accggcgctc cgagaatgga ctggcgctgg
     2461 cgcggcacat tcgtcgtcat cgccgcgtat gctaccgtac cgggttggta attggcccgc
     2521 atgagaggac tgcccgcggc atgacggcct tcccgcccgc tcccgacgcc cagctcgcca
     2581 gccggttgct ggacgtgctg gttcgccacc ggcaggtgcc agaccagacc ggctggcggg
     2641 ccgaggtcgc tcccggcggc tcgaccgacc gcaccttccg catcctcgac ccgaccggcg
     2701 ccccgcgcct gacggcccga ctggcccggc ccggtctcac cgagcacctg cgccacgagc
     2761 agcgcacgct gctggaactg gccggcgagg cgacgccgtc aaccacctgc gcctcgcccc
     2821 gcgatgtcgt cctgatcgaa gacgacgggc tggccgagca gatgctgctc gttcacgcgc
     2881 acatgcctgg gaatccacat ccactgggcg acgtctcgtc cgatgcgcgc gagcggctgg
     2941 ggagttgtct ggcctgggtt cacggccacc aacgcgaggg ctacacgatc tggccgtcgc
     3001 tggagggcaa cgtcggtacc cgcgccgatc tctaccacga ccggctggcg accttgcgcc
     3061 ggtaccagtc tgcagaccaa ctcacggacg ccggaacgct gctggacagg ctgacacgtg
     3121 ccgacctgcc ggccagcgcc ggctggcacg aac
//
LOCUS       DASUGJ010000218         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_169406, whole genome shotgun sequence.
ACCESSION   DASUGJ010000218 DASUGJ010000000
VERSION     DASUGJ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169406"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="rlmN"
                     /locus_tag="VFV93_04840"
     CDS             complement(<1..722)
                     /gene="rlmN"
                     /locus_tag="VFV93_04840"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5164702.1"
                     /translation="MKTIATETTTTTNDMIRPTGRARFRPHSVFEYTLDELADWFTER
                     GEPAYRAKQLYNWVFRQLATDYGQVTVFPAGMRARLAVDLPMQALTPIHEIATDDGET
                     LKVLYRTVDGQTLETVLMFYPDRSTVCVSCQVGCAVGCSFCATGLMGLHRNLSAGEMV
                     AQVVDMARRSSEQGRPLTNIVMMGMGEPFHNYDNVMKMVAILNQPEGMHFGSRRITVS
                     TSGVVPFIDKLAGEPYQVNLAIS"
     gene            827..1636
                     /locus_tag="VFV93_04845"
     CDS             827..1636
                     /locus_tag="VFV93_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5164703.1"
                     /translation="MAIDWSEVEKIVAGAEERGTVGVTIVAPSGERWSHNGSRQFKAA
                     SCVKIPLMVQIYRQIDRGERKLDDTFVLRDSEKAVGSGVMLHLHDGIEMTVNDLLYLM
                     ISISDNTATNVLIDMAGMDDVNAVMRELEMTGSNLGRKMKGRPAIEGETENLATSDDY
                     VAVVEAILDGRAASAESCEAMTAMLEKQSNTRRISRYLPEDDAIRWGTKTGSIKGVTN
                     DAGFVMTDSGRLVIAVFCENLPDQHTGEQVIGDITRAALKATGLVEPLYTS"
     gene            complement(1663..2103)
                     /locus_tag="VFV93_04850"
     CDS             complement(1663..2103)
                     /locus_tag="VFV93_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164704.1"
                     /translation="MRNIVAGLALVLLLAGCGIGGNDEPASDATSRLIDAFSKSDYAT
                     AYDMLHSAQRKLIDEQVFIDCDKQREMSSPAKVDSYEITGSVKRSRDVARIGDKEVTE
                     VGANLTQGTNVSYRTWDMLKDGGEWSWVLSADDLNAFQEGRCPG"
     gene            2335..2910
                     /locus_tag="VFV93_04855"
     CDS             2335..2910
                     /locus_tag="VFV93_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5164705.1"
                     /translation="MPQFGRLFRGRRSSRQTYRSLLHAFSSLVALSVIVIVLAACAGV
                     NDSGTHPDFVQTQAAGHADEGGENGEGSPSPGNEATAPAEGSPSEGEGGGEGDAANGQ
                     ALATSNGCTGCHSIDGSATVGPSWKGLYGHEVKLSDGSTVTADDAYITESIHDPGAKI
                     VDGFQNIMPPTFANMSESDVNDIIAYIKTLE"
BASE COUNT          570 a          958 c          943 g          512 t
ORIGIN      
        1 gagatcgcca ggttgacctg atacggctca ccggccagct tgtcgatgaa cgggacaacg
       61 ccggaggtcg agacggtgat ccgccgcgag ccgaagtgca tgccctccgg ctggttcagg
      121 atcgcgacca tcttcatgac gttgtcgtag ttgtggaatg gctcgcccat ccccatcatg
      181 acaatgttgg tcagtggccg gccctgctcg ctggaacggc gggccatgtc gacgacctgt
      241 gccaccatct cgccggcgct caggttccgg tgtagcccca tcaggcccgt cgcgcagaac
      301 gagcagccga cggcgcagcc cacctgacac gagacgcaga cggtcgagcg gtcggggtag
      361 aacatcagga ctgtttcgag ggtctggcca tcgacggtcc ggtacagtac cttcagcgtc
      421 tcgccgtcgt cggtcgcgat ctcgtgaatc ggcgtcagtg cctgcatcgg cagatcaacg
      481 gccaaccgcg cccgcatgcc ggccgggaag accgtcacct gcccatagtc ggtcgcgagc
      541 tgtcggaaga cccagttgta gagctgcttc gcccgatatg ccggctcgcc ccgctccgtg
      601 aaccagtccg ccaactcgtc gagcgtgtac tcgaagacgc tgtgtggtcg gaagcgtgcc
      661 cgtccggtgg ggcggatcat gtcgttggtt gtcgtggttg tctcggttgc gatcgtcttc
      721 atcgcccaag tataccgcag ttggcgctcg ttatgttgag tgacgcggat tcgattgcga
      781 gtatgatgct gccggctgga atcggatttt ggagagggag gaaccgatgg caatcgactg
      841 gagcgaggtc gagaaaattg tcgctggcgc tgaagaacgt gggacagtcg gcgtgacgat
      901 cgtcgcgcca agtggcgagc gctggagcca caacggcagc cggcaattca aggcggccag
      961 ttgcgttaag atcccgctga tggtccagat ctaccggcag atcgaccgcg gtgagcgcaa
     1021 gctggacgac acgttcgtgc tgcgcgacag cgagaaggca gtcggcagcg gtgtcatgct
     1081 ccacctgcac gacggcatcg agatgaccgt caacgacctg ctctacctga tgatctccat
     1141 ctcggacaac acagcgacga acgtgctgat cgacatggcc gggatggacg acgtcaacgc
     1201 cgtcatgcgc gaactggaga tgaccggctc gaacctcggc cgcaagatga agggacgccc
     1261 ggcgatcgaa ggcgagacgg agaacctcgc cacctccgac gactatgtcg ccgtcgtcga
     1321 ggccatcctg gatggcagag cggcgtcagc ggaatcgtgc gaggcgatga ccgccatgct
     1381 ggagaagcag agcaacaccc gccgcatctc ccgctacctg cccgaggatg acgcaatccg
     1441 ctggggcacg aagaccggca gcatcaaggg cgtcaccaac gacgccggat tcgtgatgac
     1501 cgattcaggc cggctggtga tcgccgtctt ctgtgagaac ctgcccgacc agcacaccgg
     1561 cgagcaggtg atcggcgaca tcacgcgcgc cgcgctgaag gcaaccggcc tcgtcgagcc
     1621 gctctacacg agctgaagca ccagccggcg agggacgctg cctcagccgg ggcagcgtcc
     1681 ttcctggaac gcgttcagat cgtcagcgct gagcacccag gaccattcgc cgccgtcctt
     1741 gagcatgtcc caggtgcggt acgagacgtt cgtaccctgg gtgaggtttg cgccgacctc
     1801 ggtcacttcc ttgtcaccga tgcgcgcgac atcgcgagag cgcttgaccg acccggtgat
     1861 ctcgtacgag tcgaccttgg cggggctgct catctcgcgc tgcttgtcgc agtcgatgaa
     1921 cacctgttcg tcgatgagct tgcgctgcgc cgagtggagc atgtcgtagg cggtcgcgta
     1981 gtcactcttg ctgaaggcgt caatcagcct gctggtcgcg tcgctggcgg gctcatcatt
     2041 gccaccaatg ccgcagccgg cgagcaacag caccagcgcc agacctgcca caatattccg
     2101 cacgacgaca tctctcccgg ctacacacct tcgacagtgg ctcagctgcc gcccctgccc
     2161 gacaatcgtc cgatttttcc acacccgacc ggcaacgtgt cccgcaggac cgcgcgcacc
     2221 gaacgacatg caagcgcgcc gaatttgtga tacgttacgc tgtggttggg ggacgttggg
     2281 aatcggcgca gcacgccgcc ggttcgtgag taatcgagac aaggaaagag acgtatgccg
     2341 cagttcggaa ggctgtttcg cggccggcgc tccagccggc agacgtaccg ttcattgctg
     2401 catgcgttct catcactggt cgcactctcc gtcatcgtca tcgtcctggc cgcttgcgcc
     2461 ggcgtcaacg acagcggcac acaccctgat ttcgtgcaga cacaggccgc cggccatgca
     2521 gatgagggcg gcgagaacgg cgaaggttcg ccctcgcccg gtaatgaggc aaccgctccg
     2581 gccgaaggct caccaagcga gggtgaaggc ggcggcgagg gcgacgctgc caacgggcag
     2641 gcgctcgcga cctcgaacgg ctgcactggc tgccactcga tcgacggctc tgcgacggtc
     2701 ggaccgagct ggaagggcct ctacggccac gaggtgaagc tgtccgatgg ctcgactgtc
     2761 acggccgacg acgcctacat caccgagtcg attcacgacc ccggcgcgaa gatcgtcgac
     2821 ggcttccaga acatcatgcc gcccaccttc gccaacatgt ccgaatccga tgtcaacgac
     2881 atcatcgcct acatcaagac gctcgaatag cgcccctgaa cccgatcccc gcgtaggggc
     2941 gatggccgca gtctcgccat gcccggatcg cacgacgaag ggc
//
LOCUS       DASUGJ010000219         5778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1976334, whole genome shotgun sequence.
ACCESSION   DASUGJ010000219 DASUGJ010000000
VERSION     DASUGJ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976334"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFV93_04860"
     CDS             complement(<1..674)
                     /locus_tag="VFV93_04860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5164706.1"
                     /translation="MQDQSATVAAVVPRVMDNYLTFWRTLAGCYDGAVEEADGLVMIA
                     TGLPIPFFNPAAILRWPADPVAVVERVRQFAGRHGCQSMLATWGEIATRFEPVARQLG
                     LVDDGAAPEMILLPTDQRPLAALEGLTIEPVTTAAGQRAFADVVAGTYAMPRELASGF
                     EAPALLTAPGIICYVGSVDGRPVATSMLLVTSDVAGVHVVGTLPEYRRRSIGAAMTQR
                     CVDDGW"
     gene            complement(955..2607)
                     /locus_tag="VFV93_04865"
     CDS             complement(955..2607)
                     /locus_tag="VFV93_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164707.1"
                     /translation="MSGYYNLGPHTRTISTTSPDAQLWFDRGLNWTYGFNHEEAVRCF
                     ERAVEADPECPMAYWGIAYAAGPNYNKSWEMFDESELSETVAKTYDAVQRAAALLDRA
                     NPAERALISALQKRYQAAEPVEDFSRWTDDYADAMRFVYRDYGHDLDVATLAAEALMN
                     QTPWQLWNLPARTPADGSSTVEARQILETALENPASRVHPGVLHMYIHLMEMSPWPEK
                     ALRAGDWLRGLVPDAGHLEHMPTHIDVLCGHYSTVVASNHKAILADNKYLEREGAMNF
                     YTLYRVHNFHFKLYGAMFLGQSEVALQTADEVVATIPPALVEVEVPPMADWLEGFLPV
                     KMHVLIRFGKWQEIIDTPLPENRSLYCVTTAMIHYARGVAYAATGRIDEAERQRDLFR
                     AAVGRVPDTRYLFNNACLDILAIASEMLDGELEYRKGNYDAAFAHLRRSIELDDGLTY
                     DEPWGWMQPTRHAYGALLLEQGHVAEAEAVYRADLGLDDSLARAFQHPENVWSLHGYH
                     ECLIRQGKHELAAMIKQRLDLAIARADVPIQSSCYCRMTHAA"
     gene            complement(2736..4397)
                     /locus_tag="VFV93_04870"
     CDS             complement(2736..4397)
                     /locus_tag="VFV93_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164708.1"
                     /translation="MSDYYNLGPHTRTISTASPDAQLWFDRGLNWTYAFNHEEAVRCF
                     EAAVAADPNCALAHWGIAYAAGPNYNKPWEYFDEVDLAQTIARCHDVTQHALTLLDDA
                     SPAERALVEALAARYQSPAPPGSVEDLVPWTDADAAAMRVVYRDYSADLDIASLAAEA
                     LMNRTPWRLWDQPTGQPVDGADTLEAKEILDRALQNPASRVHPGVLHMYIHLMEMSPA
                     PEAALRPADFLRGLVPDGGHLQHMSTHIDILCGHYHDVIEWNQRAMDVDETYFAHEGA
                     MNLYTMYRVHNVHFKLYGAMFLGQSDVALEAADRMIAMIPEDLLRVEIPPMAWWLEPF
                     VPMKMHALIRFGKWQEIIELTLPADQQLYCVTTAMIHYAKSVAFAATGRIAEAEDEQR
                     LFDAAVSRVPEQYQFQNTPCRDIFALAGEMLAGELEYRRGNHAVAFDHLRKSVELEDN
                     LPYAEPWAWMQPTRHALGALLLEQGHVEEAEAVYRADLGLDDTLPRSCQHPENVWALH
                     GYHECLVRLGKLDLATMIRQRLDLAVARADIPIEASCFCRMSQAA"
     gene            complement(4492..>5778)
                     /locus_tag="VFV93_04875"
     CDS             complement(4492..>5778)
                     /locus_tag="VFV93_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164709.1"
                     /translation="PDLQVWSDDYADAMRKVYRDHGHHLDIAALFAEALMNRTPWRLW
                     DLPARGPAEGSSTLEAKEVLERALENPASRQHPGVLHMYIHLMEMSPWPERALKVADW
                     LRELVPDAGHLRHMSTHIDVLCGHYQTVVDCNQAAIDADRKFLERQGAMNVYTLYRTH
                     NVHFKLYGAMFLGQSEVALEAADEMTATIPVELLQVQVPPMADRLEGRFAIKPHALIR
                     FGRWQEIIEMSLPEDQQLYSVTTAMLHYAKGVAYSATGRIAEAEEQQALFEAAVGRVP
                     ETRFFQNNSCLDILGVASAMLAGELEYRKGNYEAAFAHLRESIDRSDNLVYSEPWAWM
                     QPPRHAYGALLLEQGHVAEAEGVYRADLGLDDTLPRAHQHPENVWSLHGYHECLQRQG
                     KHELAAMIRQRLDLAIARADVPIEASCYCRASCCAA"
BASE COUNT          964 a         1875 c         1916 g         1023 t
ORIGIN      
        1 ctccaaccgt catcgacaca gcgctgagtc atcgccgcgc cgatactgcg gcggcggtac
       61 tcgggcagcg tcccaacgac gtggaccccg gcaacatcgc tcgtcaccag cagcatcgag
      121 gtcgcaaccg gccggccgtc caccgacccc acgtagcaga tgatgccggg agccgtcagc
      181 agtgccggcg cttcgaaccc gctggcaagc tctcgtggca tcgcgtaggt gcccgccacg
      241 acatcggcga aggcacgctg cccggccgcc gtcgtgacag gctcgatggt gagtccttcg
      301 agcgctgcaa gcggccgctg atccgtcgga agcaggatca tctcgggcgc cgcgccatcg
      361 tctaccagtc caagctgccg ggctactggt tcgaatcgtg tcgcaatctc accccaggtc
      421 gccagcatcg actgacagcc gtgccgcccg gcgaactggc gcacccgctc gacaaccgcg
      481 accggatcag cgggccacct gaggatcgcg gccggattaa agaagggtat cggcaggccg
      541 gtggcgatca tcaccagtcc gtctgcttcc tcgaccgcac cgtcgtagca gccagccaaa
      601 gtccgccaga acgtcaggta gttgtccatc actcgcggca cgacggccgc aactgtcgct
      661 gactggtcct gcattccatc tccgatcgtg atgcgtgacg cgtggcgcgg agcagacaaa
      721 cggtcttcga gaggaccagg gcatcttccc tgtcatcctg agcggcagcg aaggatcgcc
      781 tgacgttggg gcaatgcgtg gccgcctagg atgcgaacgc tcgacgacca agtaagctgc
      841 tcgcgtctct tacgccaggc gatccttcgc tgtcgctcag gatgacatgg caagtgggtg
      901 cggtcctcgt gagagctatt cgcctgatcc acaatacgca tcacgcatca cacatcacgc
      961 cgcatgcgtc atccggcagt aacaggatga ctggatcggg acgtcggcgc gggcgatggc
     1021 gaggtcgagg cgttgcttga tcatcgccgc cagctcgtgc ttgccctggc ggatcaggca
     1081 ctcgtggtag ccgtgcaggc tccagacgtt ttccgggtgc tggaaggcac gcgccaggct
     1141 gtcgtccagc cccagatcag cgcggtagac ggcctcggcc tccgcgacgt ggccctgctc
     1201 cagcaggagc gcgccgtagg cgtggcgggt cggctgcatc cagccccacg gctcgtcgta
     1261 ggtcaggccg tcgtccagct cgatcgaccg acggagatgg gcgaaggctg cgtcgtagtt
     1321 gcccttgcgg tactccagct cgccgtccag catctcggac gcgatcgcga ggatgtccag
     1381 acacgcgttg ttgaacaggt agcgcgtatc aggcacgcgg ccgacggcgg cccggaacag
     1441 gtcacgctgc cgctcggcct cgtcgatccg gccggttgca gcgtacgcga cgcccctggc
     1501 gtagtggatc atcgccgtcg tgacgcagta gagcgaccgg ttctccggca acggcgtgtc
     1561 gatgatctcc tgccacttgc cgaagcggat caggacgtgc atcttgaccg gtaggaatcc
     1621 ttccagccag tcggccatcg gcggcacctc gacctcgacc agcgccggcg ggatcgtcgc
     1681 gacgacctcg tcagcggtct ggagtgcgac ttctgactgg ccgaggaaca tcgccccgta
     1741 gagcttgaag tggaaattgt ggacgcggta gagcgtgtag aagttcatcg ccccttcgcg
     1801 ctccaggtat ttgttgtcgg ccaggatcgc cttgtggttg gaggcgacga cagtgctgta
     1861 atggccgcag aggacgtcga tgtgggtcgg catgtgctcc agatggccgg cgtccggcac
     1921 cagcccgcgc agccagtcgc cggctcgcag cgccttctcc ggccacggcg acatctccat
     1981 cagatggatg tacatgtgca gcacaccggg gtggacgcgg ctggccgggt tctccagcgc
     2041 tgtctcaagg atctggcgcg cctcgaccgt actggagcca tcggccggcg tccgggccgg
     2101 caggttccag agttgccagg gcgtctggtt catcagtgcc tcggcggcga gcgtcgcgac
     2161 atccagatcg tggccgtagt cgcggtagac gaagcgcatt gcgtcggcgt agtcatcggt
     2221 ccagcgcgag aagtcctcga ccggctcggc agcctgatag cgtttctgga gggcgctgat
     2281 cagtgcccgc tcggccggat tggccctgtc gagcaaggct gcggcacgct ggaccgcgtc
     2341 atacgtcttc gcaaccgtct ctgacagctc ggactcgtcg aacatctccc acgacttgtt
     2401 gtagttcggc ccggcggcgt aggcgatacc ccagtacgcc atcggacact cagggtcagc
     2461 ctcgaccgcc cgctcgaaac agcgcaccgc ttcctcgtgg ttgaacccat atgtccagtt
     2521 caatccccgg tcgaaccaga gctgtgcgtc cggagaggtc gttgaaatcg tcctggtatg
     2581 tgggccgaga ttgtaatagc cggacatgtg gtttctcctt cgtgtcggga ttcgggattc
     2641 tggacttggg attcgggatt cggagatgtt acagggacat gccccttccg atacccccga
     2701 atcccgagtc ccgagtcccg aatcccggtc tacgctcagg ccgcctgact catccggcag
     2761 aagcacgacg cttcaatcgg gatgtcggcg cgggcgacgg cgagatcgag gcgctggcgg
     2821 atcatcgtcg ccaggtcgag cttgccgagc cggacgaggc actcgtggta gccgtggagc
     2881 gcccagacgt tctccgggtg ctggcaggaa cggggcagcg tgtcatcgag cccgagatcg
     2941 gcgcgataga ccgcctcggc ctcttcgaca tggccctgct ccagcagcag cgcgccgagg
     3001 gcgtggcggg tcggctgcat ccaggcccac ggctccgcgt acgggaggtt gtcctccagc
     3061 tcaaccgact tccgcaggtg atcgaaggcg acggcatggt tgcccctgcg gtactccagc
     3121 tcgccggcca gcatctcgcc cgcgagcgcg aagatgtcgc gacacggagt gttctggaac
     3181 tgatactgct caggcacgcg actgacggcc gcgtcgaaca gccgctgctc gtcctcagcc
     3241 tcggcgatcc ggcccgtcgc cgcgaaggcc acgctcttcg cgtagtggat catcgcggtc
     3301 gtgacgcagt agagctgctg atcggccggc agtgttagct cgatgatctc ctgccacttc
     3361 ccgaagcgga tcagcgcgtg catcttcatc ggcacgaacg gctccagcca ccaggccatc
     3421 ggcggaatct cgacccgcag cagatcctcg gggatcatcg cgatcatccg atcggcagcc
     3481 tccagcgcga cgtcggattg gccgaggaac atcgcaccgt agagcttgaa gtggacgttg
     3541 tggacgcgat acatcgtgta gaggttcatg gcgccctcgt gcgcgaagta ggtctcatcg
     3601 acatccatcg cccgctggtt ccactcgatc acgtcgtggt agtgcccgca gaggatgtcg
     3661 atgtgggtcg acatgtgctg gaggtggccg ccgtccggca cgagcccgcg caggaagtcg
     3721 gctggccgca gcgctgcttc cggcgccggt gacatctcca tcagatggat gtacatgtgc
     3781 agcacgccgg gatggacgcg gctggccggg ttctggaggg ctcggtcgag gatctccttc
     3841 gcctcaagcg tgtcagcgcc atcgaccggc tggccggtcg gctggtccca gagccgccac
     3901 ggagtccggt tcatcagcgc ctcggcggcc agcgaggcga tgtcgaggtc ggcgctgtaa
     3961 tcgcggtaga caacgcgcat cgcagcagca tcggcatcgg tccacggcac gagatcctcg
     4021 accgagccgg gaggcgccgg cgactgatag cgcgcggcga gcgcctcgac cagcgcccgc
     4081 tcggccgggc tggcgtcgtc cagcaacgtc agcgcgtgct gggtgacatc gtgacagcgc
     4141 gcgatcgtct gggccagatc aacctcgtcg aagtactccc acggcttgtt gtagttcggc
     4201 ccggccgcgt aggcgatacc ccaatgggcg agcgcgcagt tggggtcggc ggcgaccgcc
     4261 gcctcgaagc agcgcaccgc ctcctcatga ttgaaggcgt aggtccagtt cagaccccga
     4321 tcgaaccaga gctgtgcgtc aggagaagcg gttgagattg tccgggtgtg tggaccgagg
     4381 ttgtagtagt cggacatcgg gttctccctt cgggacggga ttcgggattt ggggatgttg
     4441 aaggggtatg aaccgtccga cacccccgaa tcccgaatct cgaatcccaa cctacgccgc
     4501 gcaacaactc gcgcggcaat aacatgacgc ctcgatcggg acatcggcgc gagcgatggc
     4561 gaggtcgagg cgctggcgga tcatcgcggc cagttcatgc ttgccctggc gttgcaggca
     4621 ctcgtggtag ccgtgcaggc tccagacatt ctccgggtgc tggtgggcac gggggagcgt
     4681 gtcgtccagg ccgaggtcgg cgcggtagac gccttcggct tcggcgacgt ggccctgttc
     4741 gagcaggagc gccccgtagg cgtgacgcgg cggctgcatc caggcccacg gctcggagta
     4801 gaccaggttg tcggagcggt cgatcgactc acgcaggtgg gcgaaggcgg cctcgtagtt
     4861 gcccttgcgg tactccagct cgccggccag catcgccgag gccacgccga ggatatcgag
     4921 acagctgttg ttctggaaga agcgcgtctc cggcactcgc ccaaccgccg cttcgaacaa
     4981 cgcctgctgt tcctcggctt cggcgatccg gcctgtagcg gagtaggcca cgcccttggc
     5041 gtaatgcagc atcgccgtcg tgacgctgta gagttgctga tcctccggca gcgacatctc
     5101 gatgatctcc tgccagcggc cgaagcggat cagcgcgtgc ggcttgatcg cgaagcgccc
     5161 ttcgagccgg tcggccattg gcggcacctg gacctggagc agctcgaccg gaatcgtcgc
     5221 cgtcatctcg tcggccgctt cgagcgcgac ttcggactgg ccgaggaaca tcgccccgta
     5281 cagcttgaag tggacgttgt gggtccggta gagcgtgtaa acgttcatcg cgccctggcg
     5341 ctccaggaac ttccggtcgg cgtcgatcgc cgcctggttg caatcgacga ccgtctggta
     5401 gtggccgcag aggacatcga tgtgcgtcga catgtggcgc aggtggccgg cgtccggcac
     5461 cagctcgcgc agccagtcgg ccaccttcaa cgcccgctcc ggccagggcg acatctccat
     5521 caggtggatg tacatgtgca acacaccggg atgctgccgg ctggcggggt tctccagcgc
     5581 ccgctccagt acctcctttg cttcgagcgt gctggaaccc tcagccgggc cacgcgcagg
     5641 cagatcccag agccgccacg gggtccggtt catcagcgct tccgcgaaga gcgccgcgat
     5701 gtcgagatgg tggccgtggt cgcggtagac cttccgcatc gcatccgcgt agtcgtcgct
     5761 ccagacctgg agatcggg
//
LOCUS       DASUGJ010000220         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_725717, whole genome shotgun sequence.
ACCESSION   DASUGJ010000220 DASUGJ010000000
VERSION     DASUGJ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725717"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VFV93_04880"
     CDS             <1..1074
                     /locus_tag="VFV93_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164710.1"
                     /translation="VMLISSSLVTITHIPEEAFEEGGEANGRALAFIAHEQLGDLFGS
                     VYDVSTILILWFAGASAMAGLLNIVPRYLPRYGMAPDWARATRPLVLILMSICFAVTL
                     AFRADVDAQAGAYATGVLAVITSATIAVTLAARSAGQRNATLFFGVIALIFIFTTAVN
                     VIERPEGLGIAIIFIVAIVGTSLVSRVFRATELRVNEVVLDETAEKLLAEAAQSGSVR
                     IIANHPDERDTREYLLKLREQREDHHIPGGSPVLFFEVSIADASEFAPALEIRGEHIG
                     EYRVLQARAASVPNAIAAFALYARDRLGVIPHVYFGWTEGNPIKYLARFVLFGEGDIA
                     PVTHEILRKAEPDRERRPAIHVG"
     gene            complement(1062..1871)
                     /gene="eutC"
                     /locus_tag="VFV93_04885"
     CDS             complement(1062..1871)
                     /gene="eutC"
                     /locus_tag="VFV93_04885"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HEX5164711.1"
                     /translation="MADEASGPGVSEDPWQTLRAVTPARIALGRSGISQPTRHHLAFQ
                     LAHARARDAVHSELDITRLRQDLVEADLESLVLSSAATDRATYLRRPDLGRRLDQASA
                     DLLAARGRAASDVVFVIADGLSALAVQRHAAPLLRRILAQIDRDGWRSGPLCIVRQGR
                     VAIGDDIGQRLGAALAVILIGERPGLSSPDSLGVYLTWNPRLDATDAERNCISNIHAA
                     GLTYDEAAQTLLYLMTEARRRRLSGVLLKDETQVTGGTTPALDEASDGQPT"
BASE COUNT          313 a          683 c          680 g          357 t
ORIGIN      
        1 gtcatgctca tctccagcag cctcgtgacg atcacgcata tccccgagga agcgttcgag
       61 gagggtggcg aggccaacgg gcgcgcgctg gccttcatcg cccacgagca gcttggcgac
      121 ctgttcggct cggtctatga cgtcagcacg atcctgatcc tctggttcgc cggtgcctcg
      181 gcaatggccg ggttgctcaa catcgtccca cgctacctgc cacgctacgg gatggcgccc
      241 gactgggccc gggcgacccg accgctggtg ctgatcctga tgtcgatctg cttcgcggtg
      301 acgctggcct tcagggcgga cgtcgacgcc caggccggcg cctacgcgac cggcgtcctg
      361 gccgtaatca cgtcggcgac gatcgcagtg acgctggctg cccgcagcgc cggtcagcgc
      421 aacgcaacgc tcttctttgg tgtcatcgcg ctcatcttca tcttcacaac ggccgtaaac
      481 gtcatcgaac ggccggaggg gttggggatc gcgattatct tcatcgtggc aatcgtcggg
      541 acatcgctgg tctcgcgcgt cttccgcgcg acggagctgc gcgtcaacga ggtcgtcctc
      601 gatgagacgg ctgagaagct gctggccgag gcggcccaga gcggcagcgt gcggatcatc
      661 gccaaccacc cggacgagcg cgatacgcgc gagtacctgc tgaagctccg cgagcagcgc
      721 gaggatcacc acatccccgg tggcagcccg gtcctcttct tcgaggtctc aatcgccgac
      781 gcgtcggagt tcgcgccggc actggagatt cgcggcgaac acatcggcga gtaccgcgtc
      841 cttcaggcgc gggcggcctc agtcccgaac gcgatcgccg cgtttgccct ctatgcccgc
      901 gaccggctgg gtgtcatccc acacgtgtac ttcggctgga ctgagggcaa tccgatcaag
      961 tatctggccc gcttcgtcct cttcggcgag ggcgatatcg cgccggtgac gcatgagatc
     1021 ctgcgcaaag ccgagccgga tcgcgagcga cggccagcca ttcacgtcgg ctgaccgtcg
     1081 cttgcctcat ctaacgcggg tgtcgtgcct ccggtgacct gtgtctcgtc cttgaggagg
     1141 actccagaaa gccggcgtcg ccgggcttca gtcatcaggt aaagcagcgt ctgggccgcc
     1201 tcgtcgtagg tcagaccggc ggcgtggatg ttcgagatgc agttgcgctc ggcgtcggtc
     1261 gcgtcgagcc ggggattcca ggtcaggtag acgccgaggc tgtccggtga actgagtccg
     1321 ggccgctcgc caatcaggat gaccgccagt gccgccccga gtcgctggcc gatgtcgtcg
     1381 ccgatcgcca cccgtccctg gcggacgatg cagagcggac cagaccgcca gccatcgcgg
     1441 tcgatctgcg cgaggattct cctcagcagg ggcgcggcgt gccgctggac ggccagcgcc
     1501 gagaggccgt cagcgatgac gaagacgacg tcactggctg cccgacctcg cgctgccagt
     1561 agatcggctg atgcctgatc cagccgccgg ccgagatctg gccggcgcag gtaggtcgcg
     1621 cgatccgtcg ccgcgctcga aagcacgagt gattcgaggt ccgcttctac cagatcttgc
     1681 cgtagacgcg tgatatcgag ttcgctgtgg acggcatcgc gggcgcgggc gtgggcaagc
     1741 tggaacgcca ggtgatgacg tgtcggctgg ctgatgccgg atcgtccgag cgcgatccgg
     1801 gccggtgtga cggctcgcag cgtttgccat gggtcctcgc tgacgcccgg cccgctcgcc
     1861 tcgtcagcca tccgatgcgg ctcctgcttc cagccgcgcc agcgttccgg cgaacggctc
     1921 cggaagctgc ccggccagtt ccggccggct gagccggtcg gaaatgccca tctcatgcag
     1981 ccagcgctcg aactccggcg cgggcttgag gccgagtacc tgccgtgcat aga
//
LOCUS       DASUGJ010000221         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_448571, whole genome shotgun sequence.
ACCESSION   DASUGJ010000221 DASUGJ010000000
VERSION     DASUGJ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448571"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..917)
                     /gene="paaC"
                     /locus_tag="VFV93_04890"
     CDS             complement(126..917)
                     /gene="paaC"
                     /locus_tag="VFV93_04890"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX5164712.1"
                     /translation="MIAHHESRTPNPKLSTQHSALSTLLLTLADDELIIGYWDTEWTG
                     IAPVLEEDVAMSSIAQDEIGHALAFYQLLAEQTGVAPDEYAYARQPEEYRFARLLGRA
                     RGDWAITIARRFLYETADAVRLASLERSSYRPLADLVAKMRREEQYHLMHVQTWYERF
                     LALPGQPRDRFSAALSDLYSDALDVFAPLDGEAELLADGTLPEPMAELRARWQAMLQP
                     SLDRAGVVVSGDAPTQPRRGGTHPDFAWLHNEMTMVYRSQPGAEW"
     gene            complement(914..1222)
                     /locus_tag="VFV93_04895"
     CDS             complement(914..1222)
                     /locus_tag="VFV93_04895"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HEX5164713.1"
                     /translation="MAIYEVFQQAREGDPMTHVGSLNAPDDELAMQYARDVFSRRYEA
                     LRLWVVPRTSVQQIDDLDLLRPPFDRSHRRPDGYNVVPRLRAIKARVAGTTQDAEAQS
                     "
     gene            complement(1249..>2012)
                     /gene="paaA"
                     /locus_tag="VFV93_04900"
     CDS             complement(1249..>2012)
                     /gene="paaA"
                     /locus_tag="VFV93_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX5164714.1"
                     /translation="RDWILRAPTLKRKLALTSKIQDEVGHAQILYRVAEDLGKPREEM
                     LADLIAGKTKFHNVFHYPTQSWADVGIIAWLVDAAAIVAQQALRDSSYAPYARAMKRI
                     CAEESVHIIHGRDVVLAMMSGTDEQQAMVQEALDRWWGPLMQMHGPPSNPEKDKDLRW
                     KIKSKHNEQLRQEFLNTYVPRLWEIGLTLPDPALRLDEETGRWHYTEPDWDELRNVVT
                     GHGPASQSRLDFRRMSIEDTAWVRAAVLGNGRVAA"
BASE COUNT          301 a          621 c          714 g          376 t
ORIGIN      
        1 tggagcgcgt cccagatatc gtgttcggag atggcggtcg atgttgtcat gatgttcagc
       61 cctcgtggta cttcgatcgg cagcgatccg tttaccttgt catcccgacg caggacgaca
      121 gggagtcacc attcggctcc cggttgcgag cggtagacca tcgtcatctc gttgtggagc
      181 caggcgaagt cggggtgcgt gccgccgcgg cgcggttggg ttggcgcgtc gccggacacg
      241 accacgccgg cgcggtcgag cgagggttgc agcattgcct gccagcgggc gcgcagttcg
      301 gccatcggct ccggcaaggt tccgtcagcg agcagttcgg cttcgccgtc gagcggggcg
      361 aacacatcga gtgcatcgga atagaggtcg ctgagggcgg cgctgaagcg gtcgcgcggc
      421 tggccgggaa gggcgaggaa gcgctcatac caggtctgga cgtgcatcag gtggtactgc
      481 tcctcgcggc gcatcttggc gaccaggtcg gccagcggcc ggtagctgga acgttcaagc
      541 gacgccagcc ggacggcgtc ggccgtctcg tacaggaagc ggcgcgcgat cgtgatcgcc
      601 cagtcgccgc gggcgcggcc gagtagccgc gcgaagcggt actcctccgg ctggcgggcg
      661 taggcgtact catcgggtgc gacgcccgtc tgctcggcga ggagctggta gaacgccagc
      721 gcatgtccga tctcgtcctg agcgatgctt gacatcgcca cgtcttcttc gagaactgga
      781 gcaatcccgg tccactcggt gtcccagtac ccgatgatga gctcgtcgtc ggccagcgtc
      841 agcaacaacg tgctgagtgc tgagtgctga gtgctgagtt taggattcgg ggttcgggat
      901 tcgtggtggg cgatcatgac tgcgcctccg cgtcttgcgt ggttccggca acgcgggctt
      961 tgatggcgcg cagcctcggg acgacgttgt agccgtcggg gcggcggtgg gagcggtcga
     1021 agggcggccg gagcaggtcg aggtcgtcga tctgctggac gctggtgcgc gggacgaccc
     1081 agagccgtag cgcctcgtag cggcggctga agacatcccg ggcatattgc atcgccaact
     1141 cgtcgtccgg cgcgttcagc gacccgacat gcgtcatcgg atcaccctcg cgcgcctgtt
     1201 gaaacacctc gtagatcgcc atcggccctc gacactcagc actcaacgtt atgccgccac
     1261 ccgcccattc ccaagtacgg cagcccggac ccaggcggtg tcctcgatcg acatgcggcg
     1321 gaagtcgagg cgggactgcg aggcggggcc gtggccggtg acgacgtttc gcagttcgtc
     1381 ccagtccggt tcggtgtaat gccagcggcc ggtctcctca tcgagacgca aggccgggtc
     1441 gggcagcgtc aggccgatct cccagaggcg tgggacgtag gtgttgagga actcctgccg
     1501 cagctgctcg ttgtgcttgc tcttgatctt ccagcgcagg tccttgtcct tctcgggatt
     1561 acttggcggg ccgtgcatct gcatcagcgg tccccaccag cggtcgagcg cctcctggac
     1621 catcgcctgc tgctcgtcgg tgccggacat catcgccagg acaacgtcgc ggccgtggat
     1681 gatgtggacc gactcctcgg cgcagatacg cttcatcgcg cgggcgtagg gggcgtagct
     1741 ggagtcacgt agcgcctgct gggcgacgat cgcggcggca tcgaccagcc aggcgatgat
     1801 gccgacgtcg gcccacgact gcgtcgggta gtggaagacg ttgtggaact tggttttgcc
     1861 ggcgatcagg tcggccagca tctcctcgcg cggcttgccg aggtcttcgg cgacccggta
     1921 gaggatctgg gcgtgcccga cctcgtcctg gatcttgctg gtcagcgcca gcttgcgctt
     1981 cagggtcggt gcccgcagga tccagtcgcg ct
//
LOCUS       DASUGJ010000222         4857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_170431, whole genome shotgun sequence.
ACCESSION   DASUGJ010000222 DASUGJ010000000
VERSION     DASUGJ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170431"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFV93_04905"
     CDS             <1..616
                     /locus_tag="VFV93_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX5164715.1"
                     /translation="DGPFGYIVETAEIRTLVEETQRLMGSVASDAERVRLLEPAFARL
                     LAAEGWLPEEYAAPDETSGMGGGIGQYALYRAEDGSLCLFSLVVPAGATTPVHDHLAW
                     GLIGLYRGRQSETVYRRLDDGSAEGQAQLEVASEQAVERGEFFALLPPDGDIHRVSTI
                     SDEPSVSIHLLANDTACVWRHSFDPDAGTVTAFRSGYSNAACVE"
     gene            complement(809..943)
                     /locus_tag="VFV93_04910"
     CDS             complement(809..943)
                     /locus_tag="VFV93_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164716.1"
                     /translation="MKRIRHTVVTILRDIEVAVIRLSGRPRPALARVPSRRPAPRERR
                     "
     gene            complement(1074..2459)
                     /locus_tag="VFV93_04915"
     CDS             complement(1074..2459)
                     /locus_tag="VFV93_04915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5164717.1"
                     /translation="MNVLVVETETSLRRSLEFALAMRGHLPESVDDAGEALAAASATH
                     YPLVIVGRVADGPESDSHAARQQLCHDLRQLPGYQHSSILVAASREEPDRIIDALDAG
                     ASGYLSRPIDAAQLRARIEDAERLAGAAPAEAPTAVASTDSVESRLMELAVDLALVLE
                     PSGRVMRVVYENPRALGSRAVPLVEVSIYALCHPEDASLAMEFLAATLDQPPGSPPNQ
                     TELRFLHQDGTWRAMDVSAANLSDDPAIGGIALVARDITHRRLAEQQIRREALYDPLT
                     SLPNRALFFTYLEHALARADRRSEPVVVMFMDIDDFAAVNASHGRETGDAILTGIGDR
                     LRTALRATDTAARMGDDEFTILLEDVGHLDEARVVAERIAEALRIPFEIGGHVIAAPA
                     SIGVAFSHPASRLPNIPSEMRLGELLRQADAALYRAKSSGKDRWVLYDATTTSRPPSS
                     LPSGSDLTRSR"
     gene            complement(2742..3098)
                     /locus_tag="VFV93_04920"
     CDS             complement(2742..3098)
                     /locus_tag="VFV93_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5164718.1"
                     /translation="MIRSARQPRYRLLLLVLVIPLVLSACGGGSGSSNDDDAGDSDQT
                     NAARDLFAAKGCADCHGPDGEGTDTAPNPLAGTRMIIQQFQTRVRNGRGSAMPAYTPD
                     QISDEEIRMLYDWLRE"
     gene            3497..4141
                     /locus_tag="VFV93_04925"
     CDS             3497..4141
                     /locus_tag="VFV93_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164719.1"
                     /translation="MDAWDEDAVAGPVEAFDATRNWSGQSVAAGRRRRSRRRPHGCLI
                     AFGVLSIVVVGLLVAALLASNYIVKPMVRDAAVADLRTGVRDEVNAQIADQIADAPEG
                     DIIITDDEINQRLAAGDLGPIDDVTITIAPDVMTVDLSAYGLHGTYQSVIRAENGTVV
                     LDSGSLDGPLAYVIPDGELTQATNAEIAAALAAAGYQVESATMQDGAIALSVVR"
     gene            4315..>4857
                     /locus_tag="VFV93_04930"
     CDS             4315..>4857
                     /locus_tag="VFV93_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164720.1"
                     /translation="MADQADGRAMISWQRNLYALWVAQMLTIIGFSLRSPFLPFYIED
                     LGASSLESQALWAGIINAGGALMMAVSAPIWGIVADRYGRKPMVLRAMFAGSVTIGLM
                     SLATSPWHLLILRFIEGGVTGTVTASMTLVASTTPKERMGFGLGMMQMAVFSGGSVGP
                     LAGGILADQIGYRATFVVAGS"
BASE COUNT          787 a         1539 c         1760 g          771 t
ORIGIN      
        1 ggacgggccg tttgggtaca tcgttgagac ggccgagatt cggacgctgg tcgaggagac
       61 gcagcggctg atggggtcag tcgcgtcgga cgcggagcgg gtgcggctgc tggaaccggc
      121 cttcgcgcgg ttgctggcgg ctgagggctg gctgccggag gagtacgccg cgccggatga
      181 gacgagcggg atgggcggcg ggatcggcca gtacgcgctc taccgggccg aggacggctc
      241 gctctgtctc ttctcgctgg tcgtcccggc cggcgcgacg acgccggtcc acgaccacct
      301 cgcctgggga ctgatcgggc tgtatcgcgg gcggcaatcg gagacggtct accgccggct
      361 cgacgacggc agtgccgaag gacaggcgca actcgaagtc gccagcgaac aggcagtcga
      421 gcggggcgag ttcttcgcgc tgttgccgcc ggatggcgat atccaccgcg tctcgaccat
      481 ctcagacgag ccatcggtct cgatccacct gctggccaac gacaccgcct gcgtctggcg
      541 ccacagcttc gaccccgacg ccggcaccgt gacagcgttc cgctccggct actcgaacgc
      601 ggcctgcgtg gagtaggtga taggtgatag gccataggtg ttaggggcta gtacatgcgg
      661 gaggaacgcg tcgcgcggac ccacacggac gaatccttaa aacctgaaac ctacggccta
      721 tcacctacat aaaaaagagg cgggtgggga gcccgcctca aaggggatag ggatggggat
      781 aggggaaggg ataggatggt tcggggtgtc agcgccgctc gcgtggggcg ggtcgccggc
      841 tggggacccg tgccagcgcc gggcgagggc ggccggagag ccggatgacg gcgacttcga
      901 tgtcgcgcag gatcgtcacg acggtgtggc gaattcgttt catccaggta cctcctctgt
      961 gatccgtcga gccggggtgt tccgcctcgt cgatctcatg tttcgagaat agatggttgc
     1021 gcgtgttccg gctgccagtc gggatacagc cggggtgtaa tctgggctac aggtcaacgt
     1081 gaacgggtca ggtcggaacc cgacggcagc gacgatggtg gccggctggt cgtcgtcgcg
     1141 tcgtagagca cccagcgatc cttgccggac gacttggcgc ggtagagcgc ggcgtcggcc
     1201 tggcgcagca actcgccgag gcgcatctca ctgggaatgt tcggcagccg cgaggccgga
     1261 tggctgaaag cgacgccgat gctggccggg gcggcgatga cgtgaccgcc gatctcgaac
     1321 gggatccgca gcgcctcggc gatccgctcg gcgacgacgc gcgcctcgtc gagatggccg
     1381 acatcctcca gcaggatcgt gaactcgtcg tctcccatcc gggcggccgt gtcggttgcg
     1441 cgcagggcgg tccgaagccg atcgccgatg ccggtcagga tggcgtcgcc ggtctcgcgg
     1501 ccgtggctgg cgttgacggc cgcgaagtcg tcgatatcca tgaacatgac gacgaccggc
     1561 tcgctgcggc ggtcggcgcg ggccagcgcg tgctccaggt aggtgaagaa cagcgcgcgg
     1621 ttcggcaggc tggtcagcgg atcgtagagc gcctcacggc ggatctgctg ctcggccagg
     1681 cggcgatgag tgatgtcgcg ggcgacgagg gcgatcccac cgatggcagg gtcgtcgctg
     1741 aggtttgcgg cgctgacatc catcgcccgc caggtgccgt cctgatgcag gaaacgcagc
     1801 tcggtctgat tcggcgggct gccaggcggc tggtccagcg tcgcggccag gaactccatc
     1861 gcgagggagg cgtcctcggg gtggcagagg gcgtagatgc tgacctcgac cagcgggacg
     1921 gcccgcgagc cgagcgcacg cggattctcg tagacgactc gcatgacccg accggacggc
     1981 tccaggacca gcgcgaggtc gacagccagc tccatcagcc gcgactcaac cgagtccgtc
     2041 gatgcgaccg cggtcggtgc ctcggccggg gcggctccgg ccagtcgctc ggcgtcttcg
     2101 atccgggcac gcagttgggc ggcgtcgatc gggcggctca gatagccgct ggcgccggcg
     2161 tcgagcgcgt cgatgatacg atccggctcc tcacgcgagg cggcgacgag gatgctgctg
     2221 tgctgatagc cggggagttg gcgcaggtcg tgacataatt gctggcgggc ggcgtgactg
     2281 tccgactccg ggccatcagc gacgcggccg acgatgacga gcgggtagtg ggtggccgac
     2341 gcggcggcca gggcctcgcc ggcgtcgtcg acgctctcgg ggaggtggcc gcgcatcgcc
     2401 agggcgaact caagcgagcg ccgcagcgat gtttcggttt cgacgaccag gacattcatc
     2461 cgtcacctcg acgccggaca cgtctgacaa cgcgggcagc cgccatggct ccagctgatg
     2521 ttgctcgtcg atcccagccg gcacacctga acccggggtg caacccccac accagtccac
     2581 ccccaacttt ggtggcacgc taccagtcta cgcacggccg tcccaaccgg ctatagccag
     2641 atggcacaga cgcggcggta cggacggatc gggacgtggg acgcgggatt cgggacgcgg
     2701 gacgcggcgg ccagacggac ggcatcccgc gtcccgaatc cctactcccg caaccagtcg
     2761 tagagcatgc ggatctcttc gtcgctgatc tggtcggggg tgtaggcggg catcgcgcta
     2821 ccgcggccgt tgcggacgcg ggtctggaac tgctggatga tcatccgggt gccggccagt
     2881 gggtttgggg ccgtgtcggt gccctcgcca tcgggaccgt ggcaatccgc gcaacctttg
     2941 gcggcgaaga ggtcgcgagc cgcgttggtc tgatcgctgt caccggcgtc atcgtcgttc
     3001 gaggagccgg agccgccgcc acaggccgag aggacgagcg gaatgacgag cacgagcagc
     3061 aggagccggt agcggggctg gcgggcagaa cggatcacga atccctcctc aaccatcagc
     3121 gatagcggct cgaccacccg agccgatgac gcgcgtgacc ggatggcggc tcatgagtta
     3181 tggcattcaa gcgctgccag cctagcagcg cgactcggcg gctgtcaaac tgaccagtct
     3241 ggcgcgcctg taagcgggtc ggagacgctg ggccagcggt gcgatacaat caacgccgtc
     3301 ctgttgacga ccaggcagcg ctccggctgc cggtgattcc cccggatggg ccatgatctg
     3361 cccccagtgc gcaacgcgaa atgagcttgg cagcaggcgc tgtcgtgcct gccgccggcc
     3421 cttcactcac gaggcctacg agtcggcagc gcctggccgc cggtcgcagc cgcgtcgcgc
     3481 ggcgtcgcca gttgcggtgg acgcgtggga tgaggacgct gttgccgggc cggtcgaggc
     3541 attcgacgcg acccgtaact ggtcggggca gtccgttgcg gccggccgca ggcggcgatc
     3601 ccgccgacgc ccgcacggct gcctgatcgc gtttggtgtg ttgagcatcg tcgtcgttgg
     3661 gctgctcgtg gcggcgctgc tggcaagcaa ctacatcgtc aagccgatgg tgcgcgacgc
     3721 ggccgtggcg gacctgcgaa ccggcgttcg cgacgaggtg aacgcgcaga tcgccgacca
     3781 gatcgcggat gctcccgaag gcgacatcat catcaccgac gacgagatca accagcggct
     3841 ggcggccggt gacctcggcc caatcgacga cgtcacgatc acgatcgcgc cggatgtcat
     3901 gacggtcgat ctcagcgcct acgggctgca tggcacctac cagtcggtca tccgggccga
     3961 gaatggaacg gtcgtcctcg acagcggctc gctggacgga ccgctggcct acgtcatccc
     4021 cgatggcgaa ttgacgcaag ccaccaacgc cgaaatcgcc gctgccctgg ccgccgccgg
     4081 ctaccaggtc gagagcgcga cgatgcagga cggcgccatc gcactcagcg tcgttcgcta
     4141 accaccgccc gcactcgtat ttggaaaagt cagagtatca tctcccccgg gatgaggggt
     4201 tggtggtccg attcacgcca gacgacggca ggcgacacaa cgtccagcga gacagccggc
     4261 acgacgagcg atgcggcctg aggcatcggc gagacgtgac ggccgggtcg cgtcgtggcg
     4321 gaccaggccg acgggcgtgc gatgatctcc tggcaacgca acctctacgc gctctgggtg
     4381 gcgcagatgc tgacgatcat cggcttcagc ctgcgttcgc cctttttgcc cttctatata
     4441 gaagacctcg gcgcgtcatc gctggaatcg caggcgctct gggccggcat catcaatgcc
     4501 ggcggggcgc tgatgatggc ggtctcggcg ccgatctggg gcatcgtcgc agaccgctac
     4561 ggacgcaagc cgatggtgct gcgggcgatg ttcgccggct cggtcacgat cgggctgatg
     4621 agcctggcga ccagtccctg gcatctgctg atcctgcgct tcatcgaggg cggcgtgacg
     4681 gggacggtaa cggcctcgat gacgctggtc gccagcacga cgccgaagga gcggatgggc
     4741 ttcgggttgg ggatgatgca gatggccgtc ttctccggcg ggtcggtcgg tccgctggcc
     4801 ggcggcatcc tggccgacca gatcggctac cgggcgacgt tcgtcgttgc cggctcg
//
LOCUS       DASUGJ010000223         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_586973, whole genome shotgun sequence.
ACCESSION   DASUGJ010000223 DASUGJ010000000
VERSION     DASUGJ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586973"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /gene="gltX"
                     /locus_tag="VFV93_04935"
     CDS             <1..1414
                     /gene="gltX"
                     /locus_tag="VFV93_04935"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5164721.1"
                     /translation="SWLYARRHGGQFILRIEDTDQRRYKEDSVTSITNALRWVGLDWD
                     EGPDIGGPNAPYYQSERLPLYQEHARTLLESGAAYECFCTSERLDALRAEQIAKKQPP
                     GYDRRCRNLSDAERGALRAEGNPPVIRFAVPLDGTTVVTDGLRGDISYENRTLEDAVL
                     LKSDGFPTYHLAVVVDDHLMGITMVTRGEEWIPSFPLHALIYEAFGWEMPPFYHMPLI
                     LNPDGKGKLSKRQGAASALAYRAEGYLPEALVNYLLLLGWSYDDKTEIFSREEMIEKF
                     DITRVNPSPARYNYEKLSWFNQYYINHIIELDDLTRRCLPFLQAAGLIGDAPDGSAAY
                     KHARDAIALIKDKMKLLTEAPQLTSFFFSDADAYEADLLIPKKTDPPVVADALQRVRN
                     IVASEGVADEAALETHLRELAGTLDLKAGQLFMPIRVAVTGRTQSPGLFETLRVIGQD
                     RVVERLDAAIAKLAGAYRDA"
     gene            complement(1426..>2244)
                     /locus_tag="VFV93_04940"
     CDS             complement(1426..>2244)
                     /locus_tag="VFV93_04940"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HEX5164722.1"
                     /translation="ERATGAIIWELGAPPLAQQHAPNMLENGNVVLLCITRLPDDIAS
                     RVIGGQPGSEHEGAIYGDYVVEMTKTGEVVWEWRAWEHLDPATHPITAAQDLRHEWTH
                     ANTVFELPDGNLMLSFRNISTVVIVERATGAIIWELGAPPLAQQHAPNMLENGNILIF
                     DNGTHRLDHPVPHSRVIEVDRATKEIVWSYQEHYPFDFFSPYISGAQRLPNGNTLICE
                     GSFGRIFEVTPEREVVWEYVNPYFVTPPLPEGSPPANRVFRAYRYPAELVMRNA"
BASE COUNT          391 a          757 c          734 g          362 t
ORIGIN      
        1 ctcctggctg tatgcccggc gccacggcgg ccagttcatc ctgcgcatcg aagacaccga
       61 ccagcgccgc tacaaagagg acagcgtcac cagcatcacc aacgccctgc gctgggtcgg
      121 gctggactgg gacgagggtc ccgacatcgg cggaccgaac gcgccgtatt accagagcga
      181 gcggctgccg ctctatcagg agcatgcccg gaccttgctc gaatccggcg ccgcctatga
      241 gtgcttctgc acgtctgagc gactggatgc cctccgcgcc gagcagatcg cgaagaagca
      301 gccgcccggc tatgaccgcc gctgccgcaa cctgagcgac gccgagcgcg gggcgctgcg
      361 ggccgagggg aacccgcccg tcatccgctt cgccgtcccg ctcgatggca caacggtcgt
      421 taccgatggc ctgcgtggcg acatcagtta cgagaaccgg acgctggagg acgctgtcct
      481 gctcaaatcg gacggcttcc cgacctacca tctggccgtc gtcgtcgatg accacctgat
      541 ggggatcacg atggtgaccc gtggcgagga gtggattccg tcgttcccgc tgcatgcgct
      601 catctatgag gcgttcggct gggagatgcc acccttctat cacatgccgt tgatcctgaa
      661 tccggacggc aagggcaagc tctccaagcg gcagggcgcc gccagcgcac tggcctaccg
      721 ggccgagggc tacttgccgg aggcgctggt caactacctg ctgctgctcg gctggtccta
      781 cgacgacaag accgagatct tctcgcgcga ggagatgatc gagaagttcg acatcacccg
      841 cgtcaacccc agcccggccc gctacaacta cgagaagctg agctggttca accagtacta
      901 catcaaccac atcatcgagc tggacgacct gacccgccgc tgcctgccgt tcctccaggc
      961 ggccggcctg atcggcgacg ccccggacgg ctcggctgcg tacaaacacg cccgcgacgc
     1021 gatcgcgctg atcaaggaca agatgaagct gctgaccgag gcgccgcaac tgaccagctt
     1081 cttcttcagc gacgccgacg cgtacgaggc cgacctgctg atcccgaaaa agaccgaccc
     1141 gccggtcgtc gccgacgccc tccagcgcgt ccgcaacatc gtcgcgagcg agggcgtcgc
     1201 cgacgaggcc gcgctggaga cgcacctgcg cgagctggcc ggcacgctcg acctcaaggc
     1261 cggccagctc ttcatgccaa tccgcgtcgc cgtcaccggc cggacccagt cccccggcct
     1321 gttcgaaacg ctgcgcgtca tcggccagga ccgcgttgtg gaacgactcg acgctgcgat
     1381 cgcgaagctg gcgggggcat atcgtgatgc gtgatgcgtg acgcgtcacg cgttacgcat
     1441 caccaactcc gccggatacc gatacgcgcg gaagacccga ttggcaggcg gcgagccttc
     1501 tgggagcggc ggggtgacga agtacgggtt gacgtactcc cagacgacct cgcgctcggg
     1561 cgtgacctcg aagatgcggc cgaaggagcc ttcgcagatg agggtgttgc cgttcggtag
     1621 ccgctgggcg ccggagatgt agggcgagaa gaagtcaaac gggtagtgct cctggtacga
     1681 ccagacgatc tccttcgtgg cccggtcgac ctcgatcacc cgtgagtgcg ggaccggatg
     1741 gtcgagccgg tgggtgccgt tgtcgaagat caggatgttg ccgttctcca gcatgttcgg
     1801 cgcatgctgc tgggccagcg gcggcgcgcc aagctcccag atgatcgcgc cggtcgcgcg
     1861 ctcgacgatg acgaccgtcg agatgttgcg gaacgagagc atcaggttgc cgtccggcaa
     1921 ctcgaagacg gtgttcgcgt gcgtccactc gtggcgcagg tcctgagcgg ccgtgatcgg
     1981 gtgggtggcc gggtcgagat gctcccaggc gcgccactcc cagacaacct cgccagtctt
     2041 cgtcatctcg acgacgtagt cgccgtagat ggcgccttcg tgctcgctgc ccggctggcc
     2101 gccgatgacg cggctggcaa tgtcatccgg gagccgcgtg atgcagagca ggacgacgtt
     2161 gccgttctcc agcatgttcg gcgcatgctg ctgggccagc ggcggcgcgc caagctccca
     2221 gatgatcgcg ccggtcgcgc gctc
//
LOCUS       DASUGJ010000224         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1420891, whole genome shotgun sequence.
ACCESSION   DASUGJ010000224 DASUGJ010000000
VERSION     DASUGJ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420891"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /locus_tag="VFV93_04945"
     CDS             <1..1716
                     /locus_tag="VFV93_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEX5164723.1"
                     /translation="DVPGHERFIKNMLAGVGGIDAALLVIASDEGPMPQTDEHLAILD
                     LLDVSRGLVVLTKRDLVDDEWLELVTEETRERLEGTALADAPIIAVSSTTGDGLPELK
                     QQLAALLSELPERAEHGKPRLPIDRAFTVAGFGTVVTGTLLGGALDVGQEIELLPDGL
                     RARVRGIQSHGRKTDRGLPGARTAVNISGVERDAISRGDVLTTPGWLEPTTLLDVRLR
                     LVRDALGPLAQNDPVDLFIGATEAPGNVTLLDSERLLPGEEGWVQIRLAQAVSAVAGD
                     RFIIRQPSPSLTIGGGRVVDPHPRRHRRFRNEVIAALETKATGTPAERLLEVLARGPA
                     ELRAVGDHLELDLAATRSLAEEAIADGTVVALGPDSTGPLAPNRLLAHAGWVERGGEG
                     IVRLLTDFHRRFPLRRGMAREEVKSRASLTGRMADAFVQTLVARGVVDDLGSLLSLPG
                     YRIELTDAQQSAADRFVAALAANPNSPPAPDGFGIAPDLLAAMADLGQIVRLPDNIVF
                     GAAQLAEIQRETMRLIDEHGSLTLAQFRDHFGSSRKYAQAVLEYFDQQRITRRVGDAR
                     VRGSA"
     gene            1716..>2188
                     /locus_tag="VFV93_04950"
     CDS             1716..>2188
                     /locus_tag="VFV93_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5164724.1"
                     /translation="MPSASSVFVAPHPDDIALSCGGTVAQAARTGHPTIVTLFAGQPP
                     EQVSDFARFQHERWGIDGASVAASRRAEDEAAARALGDSVSQTWLDHLDAIYRQAAYA
                     SDDALFGTLHDDDLPLIDVLVAELAALDAAEYVVPLAAGNHVDHQLAFRAGRRLAT"
BASE COUNT          325 a          785 c          745 g          333 t
ORIGIN      
        1 gatgtaccgg gtcacgagcg cttcatcaag aacatgctgg ccggcgtcgg cgggatcgac
       61 gccgccctgc tggtgatcgc ctccgacgag ggtccgatgc cgcagaccga cgagcatctg
      121 gcgatcctcg acctgctgga cgtgtcgcgc ggcctggtcg tcctgaccaa gcgcgatctg
      181 gtcgacgatg agtggctgga actggtcacc gaagaaacgc gcgagcggct ggaagggacg
      241 gcgctggccg acgctccgat catcgccgtc tcgtcgacga ccggcgacgg gctccccgag
      301 ctgaagcagc agctggcggc gttgctgtcc gaactgcccg aacgggccga gcacggcaag
      361 ccgcggctgc cgatcgaccg cgccttcact gtcgccggct tcgggacggt cgtgaccggg
      421 acattgctgg gcggtgcgct agacgttggc caggagatcg agctgctgcc ggacggcctg
      481 cgcgcccgcg tgcgtggcat ccagagtcac ggccgcaaga ccgaccgcgg cctgcccggc
      541 gcgcgaacgg ccgtcaatat ctccggtgtc gaacgcgacg cgatctcgcg cggcgatgtc
      601 ctgacgacgc cgggctggct ggagccgacg acgctgctcg atgtccgtct gcggctcgtg
      661 cgcgacgcgc tgggtccgct ggcgcagaac gacccggttg acctcttcat cggcgccacc
      721 gaggcacccg gtaacgtcac gctgctggat tcagaacggt tgttgcccgg cgaagagggc
      781 tgggtccaga tccggctcgc ccaggcggtg tccgccgtcg ctggcgaccg cttcattatc
      841 cgccagccct cgccgagcct gacgatcggt ggcgggcgtg tcgtcgatcc ccatccgcgc
      901 cgccaccgcc gcttccgcaa tgaggtgatc gccgcgctgg agacgaaggc gaccgggacg
      961 cccgccgaac ggctgctgga ggtgctggcg cgcggcccgg ccgaactccg cgccgtcggc
     1021 gaccatctgg aactggatct ggccgcgacc cgtagcctgg ccgaggaggc tattgcggac
     1081 gggacggtcg tcgcgctcgg tcccgattcc accggtccgc tcgcgccgaa ccgcctgctg
     1141 gcgcatgccg gctgggtgga gcgcggcggg gagggcatcg tcaggctgct gaccgacttc
     1201 caccgccgct tcccgctccg gcgcggaatg gcccgcgagg aggtcaagag ccgcgccagt
     1261 ctgacaggca ggatggcgga cgcgttcgtc cagacgctgg tggcacgtgg tgtcgtggac
     1321 gacctcggca gcctgctgtc actgcccggc taccggatcg agctgaccga cgcgcaacag
     1381 tcagccgctg accgcttcgt ggccgcgctg gctgccaacc cgaactcccc gcccgcgccg
     1441 gatggcttcg gcatcgcgcc agatctgctg gccgcgatgg ccgacctggg ccagatcgtg
     1501 cggctgccgg acaacatcgt cttcggcgcg gctcaactgg ccgagatcca gcgcgagacg
     1561 atgcggctga tcgacgagca cggcagcctg acgctggcgc agttccgcga tcacttcggc
     1621 agcagccgta agtacgccca ggccgtcctc gaatacttcg accagcagcg catcacccgc
     1681 cgcgtcggcg atgcccgcgt tcggggcagc gcctgatgcc gtctgcgtcg agcgtcttcg
     1741 tcgccccgca cccggacgac atcgccctgt catgtggcgg caccgtcgcc caggccgccc
     1801 gaactggaca tcccacgatc gtcaccctct tcgccggtca gccgcccgaa caggtcagcg
     1861 acttcgcccg cttccagcac gagcgctggg gcatcgatgg tgcgtcggtc gccgcatccc
     1921 gccgcgcgga agacgaggca gccgcccgcg cactcggcga cagcgtcagt caaacctggc
     1981 tcgaccacct cgacgcgatc taccgccagg cagcgtacgc atcggacgat gccctcttcg
     2041 gcacattgca cgacgacgac ctgccgttga tcgacgtgct ggtagcggaa ctggccgcat
     2101 tggacgctgc cgagtacgtt gtccctttgg ccgccgggaa ccacgtcgat caccagctcg
     2161 cgttccgggc cggacgtcgc cttgccac
//
LOCUS       DASUGJ010000225         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_727014, whole genome shotgun sequence.
ACCESSION   DASUGJ010000225 DASUGJ010000000
VERSION     DASUGJ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727014"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..900
                     /locus_tag="VFV93_04955"
     CDS             169..900
                     /locus_tag="VFV93_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX5164725.1"
                     /translation="MLDFSQYRVLTFDCYGTLIDWERGILNALRPVLDRHGVTLTDDE
                     ALEHFGELESAAERGEYLPYREVLTVVLAGFAERFGFTLADDERLALASSVGDWPPFP
                     DTVAGLQALARHYRLVILSNVDDDLFALSARHLKTEFADVITAQQVGSYKPNPRNFEV
                     ALERVGVPTEQVLHVAQSLFHDMEPANAIGLTTVWVNRRHNRPGFGATPPASAQPDLE
                     VPDLQTLARLVDEQHDGTGKAGDNA"
     gene            897..2075
                     /locus_tag="VFV93_04960"
     CDS             897..2075
                     /locus_tag="VFV93_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5164726.1"
                     /translation="MTDAKGPQTNDWDLDTLLIHGGGLDAETKGVAPAIHPSATFVRE
                     RIDDDARYRYARGDNPTRAQLEAILAELEHGAACSAYSSGMAAVTAALQLLSGGDHAV
                     VGYDCYLSTYNLFANDLPRYGVEADFVDLTDLEAVQAALKPTTRMLWIETPTNPLLDI
                     VDLAAIAEIAQSAGAITVVDNTFASPYCQTPIDQGIDLVVHSTTKYLGGHSDLTGGAV
                     ISRTPELAARLKDRQYYLGAVPSVFDCWLLMRGIRTLGVRMRAHMANAQAIAEALRDH
                     SKVTRVLYPGLPEHPGHEVAKRQMTGGYSGMVSFEVEGGSAAARRVSERTRVFRLATS
                     LGSVESLIYPPTAWLETDPELMAQIPGSPWAQNPGLIRLSVGIESTRDLIADLEQALG
                     "
BASE COUNT          418 a          811 c          769 g          386 t
ORIGIN      
        1 caccgtgtag gggccggtac aggacgcggc ccctacacgg tgacgacatc gcgtcacgtg
       61 tcccgcatca cgcatcacgc gtcccacgtc acgcgtcacc aatccctgcg ccccgcctca
      121 cagtatgatc ttccccagca agctcgtttg atcggagagg gcaactgaat gctcgatttc
      181 agccagtacc gcgttctgac cttcgactgt tacggcacgt tgatcgactg ggaacggggc
      241 attctcaacg ccctgcggcc ggtgcttgac cggcacggtg tcacgctgac ggatgatgag
      301 gcgctggaac actttggcga gctggaatcg gcggctgagc gcggcgagta cctgccctac
      361 cgcgaggtgc tgacggtcgt cctggccggg ttcgcggagc ggtttggctt cacgctcgcg
      421 gacgatgagc ggctggcact ggcaagctcg gtcggcgact ggccgccatt ccccgatacg
      481 gtcgccgggt tgcaggcgct ggcgcgtcac taccggctcg tcatcctctc gaacgtcgat
      541 gacgatctgt tcgccctttc ggcgcggcac ctcaagaccg aattcgcgga tgtcatcacc
      601 gcccagcagg tcggtagcta caagccgaac ccgcgcaact tcgaggtcgc gctggagcgg
      661 gtcggcgtcc caacagagca ggtgctgcac gtcgcccaga gcctgttcca cgacatggaa
      721 ccggccaacg cgatcgggct aacgaccgtc tgggtcaacc gccgccacaa ccggccgggc
      781 ttcggcgcga cgccccccgc cagcgcccag ccggaccttg aggtgccgga tctccagacg
      841 ctcgcccggc tggttgacga gcagcatgac ggaacaggga aggctggaga caacgcatga
      901 ccgacgcaaa ggggccgcag accaacgatt gggatctcga cacgctgctg atccacggcg
      961 gcgggctgga cgctgagacg aagggcgtcg ccccggctat tcaccccagc gcgaccttcg
     1021 tccgcgagcg catcgacgac gacgcccgct atcgctatgc gcgcggcgac aacccgaccc
     1081 gcgcgcaact ggaagcgatt ctcgccgaac tggagcacgg cgcggcctgc tcggcctata
     1141 gttccgggat ggcggccgtc acggccgcgc tgcaactgct ctccggcggc gaccatgcag
     1201 tcgtcggcta cgactgttat ctgagcacct acaacctgtt cgccaacgac ctgccgcgtt
     1261 acggcgttga ggccgatttc gtcgacctga ccgacctcga agccgtccag gcggccctga
     1321 agccgacgac gcggatgctc tggatcgaga cgccgaccaa cccgctgctg gatatcgttg
     1381 acctggccgc gattgccgag atcgcgcagt cggccggggc catcaccgtt gtcgataaca
     1441 ccttcgcctc gccctactgc cagacgccga tcgaccaggg tatcgacctc gtcgtccaca
     1501 gcacgaccaa gtatctcggc ggccacagcg acctgacggg aggagccgtc atctcgcgga
     1561 cacctgagct ggcggccagg cttaaggacc gccagtacta cctcggcgcc gtcccgagcg
     1621 tcttcgactg ctggctgctg atgcgcggca tccgcacgct tggcgtcagg atgcgggcac
     1681 acatggccaa cgcccaggcc atcgccgaag cgctgcgcga ccactcgaag gtgacgcgcg
     1741 tcctctaccc tggcctaccg gagcatcccg gccacgaggt ggcgaagcga cagatgaccg
     1801 gcggctacag cgggatggtc tcgttcgagg tcgagggcgg ctcggcggca gcccggcgcg
     1861 tctccgagcg gacgcgcgtc ttccggctcg ccaccagcct cggcagcgtc gagtcgctga
     1921 tctacccgcc gaccgcctgg ctcgaaaccg acccggagct gatggcccag atccccggct
     1981 ccccctgggc acagaacccc ggtctgatcc gcctctcggt cggcatcgag agcacccgcg
     2041 acctgattgc cgacctggag caggcgctgg ggtaacgcga gatccaacat ggctggttcg
     2101 acgctccgct cacgaccggc tatcgtggca tggcgcgagt gggtggccag gaatgtcgag
     2161 aatcggtcgc cggcttcgtc tcaggagtac gagcggcgac gatgggccgc acgacgacga
     2221 aggagagaca gcatgaccgt tctgcggatg gacaacgtcc tgatcgttgt caacgatctt
     2281 gaggcagcta tcgcgttctt taccgagctc ggtatggagc tggagggcaa gacgaccgtt
     2341 gagggggatt gggctggtcg tgtgatcggg ctcaacgacg tccg
//
LOCUS       DASUGJ010000226         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_32143, whole genome shotgun sequence.
ACCESSION   DASUGJ010000226 DASUGJ010000000
VERSION     DASUGJ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32143"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VFV93_04965"
     CDS             <1..1318
                     /locus_tag="VFV93_04965"
                     /inference="COORDINATES: protein motif:HMM:NF027993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164727.1"
                     /translation="DYGVTAAHLVTLNGLDHQIASPFWEFMNSHGTVQEDGQLVDDAL
                     FVDPFYATGYPLAEPYWATVALGGVSTDVLIQCFERRCLTYTPGNEPGWQVETGNVGR
                     HYEQWRYVDHAATPTATTTASATTAAETATSTPETPGATVTSTATATRTATPTRTATP
                     TRTPTVEGGDDGATPTPADGEYSESGRARKVPWSGHWFPFQNSASLNLYDDNGPLEKY
                     DEYVEETRGHNPGAKSWEAANHSGGLGWWGHCHAWAAASIMEPEPHEVTKEGIKFRQD
                     DMEGLIIETYYSPSNRIWGTECRDCADGSPAYMDVTPAVFDQIMREEMGNKRNNIIMD
                     LDPGAAVWNYPSYAYQRTSVMSRGVETVTMKVTISVPRTNVTGNDPDTVTYTYTLRDG
                     TNGQWTGGSVDDHPDFIWVVTGRTKSGGSGQANPEVDYQIVKEIQR"
     gene            1318..1965
                     /locus_tag="VFV93_04970"
     CDS             1318..1965
                     /locus_tag="VFV93_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164728.1"
                     /translation="MRHPLGRRLFAGLLLACLLLAACGGDDDSGPSSTRKGTAVPTLP
                     PTQVPATALPSPSASNPFRGDSRVNDAGGDVSGSGEAPPGIDLTGVRVVPSEDQLVII
                     WYTAQRAERTVPDGATASWTVALSNGDIPAYDITFKVSGKEWDILVLDHATGEETKHR
                     IGSIYNDRLDVPYPASKLDNLQPTFTWTAASSYTDATGATWTDTVPDQGSASFPG"
BASE COUNT          427 a          781 c          738 g          326 t
ORIGIN      
        1 cgactacggc gtgacggccg cccacctggt cacgctgaac gggctggacc accagatcgc
       61 gtcgccgttc tgggagttca tgaactccca cgggacggtc caggaggacg gtcaactggt
      121 tgatgacgcg ctgttcgtcg acccgttcta cgcgaccggc tacccgctgg ccgaaccgta
      181 ctgggcgaca gtggcgctgg gtggcgtctc gaccgacgtg ctgatccagt gcttcgagcg
      241 gcgctgcctg acgtacacgc ccggcaacga acccggctgg caggtcgaaa ccggcaacgt
      301 cgggcggcac tacgagcagt ggcgctacgt cgaccacgcc gcaacaccaa ccgcgacaac
      361 cacggcatcg gcgacgacag cggccgagac ggccaccagc acaccggaga cgccgggtgc
      421 gacggtgaca tcgacggcaa cggcgacccg gacggcgaca ccgacccgga ccgcgacgcc
      481 aacccggacg ccgaccgtcg agggcggcga cgacggcgcg acaccgaccc cggccgacgg
      541 cgagtattcc gaatcgggcc gggcgcgcaa ggtgccgtgg agcggccact ggttcccatt
      601 ccagaactcg gcctcgctga acctgtacga cgacaacggc ccgcttgaga agtacgacga
      661 gtacgtcgag gaaacccgcg gccacaaccc cggcgcgaag tcgtgggagg cggccaacca
      721 ctccggcggc ctcggctggt ggggccactg ccacgcctgg gcggctgcct cgatcatgga
      781 accggagccg cacgaggtca ccaaggaagg gatcaagttc cgccaggacg acatggaggg
      841 gctgatcatc gagacctact actcgccgag caatcgcatc tggggcacgg agtgccgcga
      901 ttgtgcggat ggcagcccgg cctacatgga cgtcacaccg gccgtcttcg accagatcat
      961 gcgcgaggag atgggcaaca agcgcaacaa catcatcatg gacctcgacc ccggcgcggc
     1021 ggtctggaac tacccctcct acgcctacca gcgcacatcc gttatgagcc ggggcgtcga
     1081 gacggtgacg atgaaagtca cgatctcggt cccgcgcacg aacgtcaccg gcaacgaccc
     1141 cgacacggtg acctacacct acacgctgcg cgacggtacc aacggccagt ggaccggcgg
     1201 cagcgtcgat gaccatccgg acttcatctg ggtcgtgacg ggccggacga agtcgggcgg
     1261 cagcggccag gcgaatccgg aggtcgacta ccagatcgtc aaggagatcc agcgctgatg
     1321 cgacacccgc tcggccggcg actattcgcc ggcctgctgc tggcctgcct gctgctcgcc
     1381 gcctgcggcg gggacgacga cagcggccca tcctcgaccc gcaagggcac ggccgtcccg
     1441 acgttgccgc cgacgcaggt gccggcgacg gcgctgccgt cgccgagcgc atccaacccc
     1501 ttccggggtg actcgcgtgt caatgacgcc ggaggcgatg tcagcggcag cggcgaggcg
     1561 ccgccgggca tcgacctcac cggcgttcgc gtcgttccct cggaggacca gctcgtcatc
     1621 atctggtaca cggcccagcg ggcggaacgg accgtcccgg acggcgcgac ggcgtcgtgg
     1681 acagtcgcct tgtcgaacgg cgatattccc gcctacgaca tcaccttcaa ggtctccggc
     1741 aaggagtggg acatcctcgt ccttgaccac gcgaccggcg aagagaccaa gcaccggatc
     1801 ggtagcatct acaacgaccg gctggatgtc ccctacccgg cctccaagct ggacaacctg
     1861 caaccaacct tcacctggac agccgccagc agctacaccg acgccaccgg cgccacctgg
     1921 accgacaccg tccccgatca aggttccgcg tcgtttcccg gctaaacgtc gtgatgcgtg
     1981 atgcgtcacg cgtcacgcgt ccaggacgta gccagatcgg gctagccgat ctggtatccc
     2041 gttctgtaac aggattgtga ccctggtcgt ttcctcttga ggccagaatt cggtaggctt
     2101 tcgaaccgcg cgttcgaatc tgtgattcgg ggcgcttgga tggggaacga tccccgcatg
     2161 ttgagcaatc tgccggcgct gtcctgcgcg ggccgggata cagggagaga tgttcatgac
     2221 tctggcgaag acacgcggcc ggatcggcgc gtcgctgctg gcgttgatgc tg
//
LOCUS       DASUGJ010000227         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1282679, whole genome shotgun sequence.
ACCESSION   DASUGJ010000227 DASUGJ010000000
VERSION     DASUGJ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282679"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..397
                     /locus_tag="VFV93_04975"
     CDS             50..397
                     /locus_tag="VFV93_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5164729.1"
                     /translation="MRQAINYAANVDGVIEAIVLGNANRVSTIIGPWIFGFDPTLAPY
                     PHDPDKAKELLTEAGYPDGIDIQIWHIQGRYPKDKEVAEAMAQEMSKAGSGLLVLTLT
                     VMSSTTSTPSMML"
     gene            complement(358..1056)
                     /locus_tag="VFV93_04980"
                     /pseudo
     CDS             complement(358..1056)
                     /locus_tag="VFV93_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1150..2199)
                     /locus_tag="VFV93_04985"
     CDS             complement(1150..2199)
                     /locus_tag="VFV93_04985"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEX5164730.1"
                     /translation="MTATPSTQAISREELAERVGTLPRVRIAHLPTPLEEAPRLRAAL
                     GPDAPRILIKRDDATGLALGGNKVRHLEFRLGDIQAKGADTLIVTNVAQSNHARLHTA
                     VAARFGLTNYIIKIPSQKDDPVNGNLLLDHIMGAQIIDAPSADPDVIDQELAALVSRL
                     EAEGHVVYNVPKDSFSKIAGACAYLLGALELLGQLDERGVKADHIFLASGTSSAGLAL
                     AGKLLGESYRVHPVSVGPSRSEIEAYVCAAANGIAELLGLDARLSADDFSVHDEYAGE
                     AYGIPTEAGMAALRLVGRSEGLILDPVYTSKAMAALVDQISQGTIDSNATVVFIHTGG
                     LPITFAYNEEVMRSI"
     gene            complement(2260..>3110)
                     /locus_tag="VFV93_04990"
     CDS             complement(2260..>3110)
                     /locus_tag="VFV93_04990"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5164731.1"
                     /translation="YLITLNIVEGFDMAGMGANSADYLHHLAEAMKLAVADRTTYAPN
                     PASPIEALLSKDYAATRRDLIDPARAGYSGGERFTSHKLPEEILQGSFESIMRESTTH
                     FATADADGWAVSCTQTLGGGFGSGVVHGNTGLSLNNFAHWFDDDPESPNVIAPNKQVE
                     MCLAPSQTWRDGRLFVTVGTPGSFGIMETTPQMLLNVLDHGYSIQAAIEAPRFRTITG
                     RELIIEGRVPEAVLDDLRRRGHEVQVLDPWTAMVGGGQGIMVDPDSGAFMAGADPRRD
                     GYALAI"
BASE COUNT          477 a         1038 c         1004 g          591 t
ORIGIN      
        1 cccgttctac ttcatcacga ctgaggtcga gccattcgac aacccgctcg tgcgccaggc
       61 gatcaactac gcggccaacg tcgatggcgt catcgaagcg atcgtgctcg ggaacgcgaa
      121 tcgcgtctcg accatcatcg gtccgtggat cttcggcttc gacccgacgc tggcgccgta
      181 tccgcacgac cctgacaagg ccaaggaact gctgaccgag gccggctatc cggacggcat
      241 cgacatccag atctggcaca tccagggccg ctacccgaag gacaaggaag tcgccgaggc
      301 gatggcgcag gagatgagca aggccgggtc cggcttgctg gtcttgacgt tgacggtcat
      361 gtcatcgacg acgtcgacgc cctcgatgat gctgtaggtg ctgcgcgcgt cgcccggcgt
      421 ctcgtcgctc agcgggcgca tgacgctgta ggcgaccgcc tcagcgttga acggctcacc
      481 gttgtggaac gtcacgcctt cgcgcaactg gaactggacc gtcaggtcgt cgacccgctc
      541 ccaggccgtc gccaagcagg gcgagatcgt cgtcaggtcg cgctcgaact tgaccagcgg
      601 ctcgtacatg tggcgcagct gcgtctcggc gatcgtaccg acgtgcagtt gcgggtcgat
      661 gccgtgtgtc tggaactgcg aggtgcccca ggcgaggatc agctcgtccg gcttgttgcc
      721 ttccggcgtg ccggccggtg ggttgtcgaa ctccttgaca tccgtcccgc gaacctcttc
      781 accttcgcca tcttcggtcg cggacgccgt ctcaccggcg gctgcttcgg ttggctcggt
      841 gctggtcgtt tcggtgccgg tcgtttcggt cgaggtctgg ccggatgtgc cgctggttga
      901 ggtcggcgca tcgtcatcgt cgtcatcgtc gtcgtcaccg ccgcaggccg ccagcaatga
      961 tgcggcgaac gtgccggcca tggcgcccag gaagagccgt ctgctcagcg tgaagttggg
     1021 cagtctgtct cgtaggttgt actccgtgtc agccatgcga gttcctcctc gtaacgacgt
     1081 tgcgaacgat gctcactctc ccccgctgat ccggcccgac aacctccttt caggacgtgc
     1141 agcctgacgt tagatgctgc gcataacctc ctcgttgtat gcgaaggtaa tgggaagccc
     1201 gcccgtgtgg atgaagacga cggtagcgtt gctgtcgatc gttccctgcg atatctgatc
     1261 tacgagcgcc gccatcgctt tggacgtgta gaccgggtcg aggatcagcc cttcggaccg
     1321 gcccaccagc cggagcgcag ccatgccagc ctcagtcggg atgccgtatg cctcgccggc
     1381 gtactcgtcg tggacgctga agtcatcggc gctgaggcgc gcgtcgaggc ccagcaattc
     1441 ggcgatccca ttcgccgccg cgcagacgta ggcttcgatc tcggaccggc tcggcccgac
     1501 gctgaccgga tgcacccgat acgactcgcc gagcagcttg ccggccagtg ccagccctgc
     1561 cgacgacgtt cctgacgcca ggaagatgtg atcggccttc acgccgcgct catcgagttg
     1621 gcccagcagc tcaagtgcgc cgagcaaata ggcacaggcg ccggcgatct tcgaaaagct
     1681 gtccttcggc acgttgtaga cgacgtgccc ctccgcctcc agccgggaga cgagggcggc
     1741 caattcctgg tcaatgacat ccggatcggc gctcggcgcg tcgatgatct gcgcacccat
     1801 gatgtggtcg agcaggagat tcccgttgac agggtcgtcc ttctggctgg ggatcttgat
     1861 gatgtagttc gtcagcccga acctggccgc gacggccgtg tgcaaccggg cgtggttgga
     1921 ctgggcgacg ttggtgacga tcagcgtgtc ggcgcctttc gcctggatgt caccgagccg
     1981 gaactccaga tgacggacct tgttaccgcc cagcgccagc ccggttgcgt cgtcgcgctt
     2041 gatcaggatt cgtggcgcgt ccggcccgag tgccgcgcgc agccgtggcg cctcttcaag
     2101 cggcgtcggc agatgcgcga tccgcacgcg cggcagcgtc ccgacccgct ccgcgagctc
     2161 ctcgcggctg atcgcctgcg tcgatggcgt cgctgtcatt cctcgccccc tcgtccgtcg
     2221 tcaccactcg accggtgcga ttcggttgtg gatgttcagc tagattgcga gcgcgtaccc
     2281 atcgcgacgc ggatcggcgc cggccatgaa cgcgccgctg tcgggatcga ccatgatccc
     2341 ctgcccgccg ccgaccatcg ccgtccacgg gtcgagcacc tgcacctcat ggccgcgccg
     2401 gcgcagatca tccagcacag cctcagggac gcggccctca atgatcagct cgcggccggt
     2461 gatcgtccgg aagcgaggcg cttcgatcgc ggcctggatg ctgtagccgt ggtcgaggac
     2521 gttgagcagc atctgcggcg tcgtctccat gatgccgaac gaccccggtg tgccgactgt
     2581 cacgaacagc cgcccgtcgc gccaggtctg cgacggcgcc aggcacatct cgacctgctt
     2641 gttcggtgcg atcacgttcg ggctctccgg gtcgtcatcg aaccagtggg cgaagttgtt
     2701 gagcgacagc ccggtgttgc cgtggacgac gcccgagccg aagccgccgc ccagcgtctg
     2761 cgtgcaggag acggcccagc cgtcggcgtc ggccgttgcg aagtgggtcg tgctctcgcg
     2821 catgatgctc tcgaagctgc cttgcaggat ctcctccggc agcttgtgcg aggtaaaccg
     2881 ctcgccgccg ctgtagccgg cccgcgcggg atcgatcaga tcgcgccgtg ttgccgcgta
     2941 gtccttcgac agcagtgcct cgatcggcga agccggattc ggtgcgtagg tcgtccggtc
     3001 cgccaccgcc agcttcatcg cctcggccag gtggtgcagg tagtcggcgc tgttcgctcc
     3061 catcccggcc atgtcgaagc cctcgacgat attgagcgtg atcagatact
//
LOCUS       DASUGJ010000228         9707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_727468, whole genome shotgun sequence.
ACCESSION   DASUGJ010000228 DASUGJ010000000
VERSION     DASUGJ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727468"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1500)
                     /locus_tag="VFV93_04995"
     CDS             complement(184..1500)
                     /locus_tag="VFV93_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164732.1"
                     /translation="MLKALSVLFVTLLLGTSLVACGGDDDDDPTATSAPANTTSATAT
                     PAAATATAGSSPTATQPAGDAATASPSGSPAAGEFTDDEAALIDMLLPADALPGDWNQ
                     QSLEAPELDQSPGVCDSPPFPRAVERIAEVEVQYQSADGASFVLEDITRFPEDVAVEA
                     MAYVRDTATCTEWTDDTGTVFRLSPADAPDLGDEAFSLHVAFDVADAGTLEGNFVFVR
                     LEGYVAIVTTLKLGEYDQEFANQIAEAATKRIDALIGTGKNVTDAEEALMGGLLTLDD
                     LDEDWDQVQPAHRSEPESWTGLCGADLFPQTAEATARVAIEFYEGFEPDSATLMQLLV
                     AYPDGISEAAYDFEVEAASCGTFTGGDTDVTLETDSAFPALGDESVAVRFSFTNDGNE
                     VEGYWIVARAGSALTTLIYTDPDQLDTATVQAIATTAVEHIQAIQQ"
     gene            1765..2997
                     /locus_tag="VFV93_05000"
     CDS             1765..2997
                     /locus_tag="VFV93_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5164733.1"
                     /translation="MVSQLPRPLPVSPIAADEVEPTRRPTERARTLPGRVYHDPDVFD
                     WERETWFSREWLFVGREEDAPETGSFFRCTVAGENVVVVRGNDGQLRAFHNVCRHRGA
                     RIVEEQSGRLVRFQCPYHAWIYDLEGNLRPPRHTETLEEFSCAAFSLFPVRCETWAGF
                     VFLNLDSAAPPLSAHLGEFPTHFDRFPLASLRRATRMEYEVRANWKALMENYAECYHC
                     PGVHPLLNHITPYNLGGYLPGAGEWAGSWMELAGDFETLSTDGYRHGRDAIPGMTQDD
                     LKRVYYAWIWPNLLFSLHPDYLMTHQVWPRDAENSSVICELYFHPETMSRPAFDASGP
                     AEFWDLTNRQDWHVCELQQAGTASRVYVPGRYAAIERMVHSFDARVADRYAGDGVRTD
                     VARPAKKEWGSGRARRSA"
     gene            complement(3063..3401)
                     /locus_tag="VFV93_05005"
     CDS             complement(3063..3401)
                     /locus_tag="VFV93_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164734.1"
                     /translation="MFDIHRGIGEGLFLVYLIIMGIIYFMSRGGREAPSWLSAISHGL
                     LALQVALGVILLADGHSVVWYHPVLGIAAMLALGLTPTLRERFGRVNGTIALMGIIAV
                     LTLAARLAVL"
     gene            3550..5217
                     /gene="polX"
                     /locus_tag="VFV93_05010"
     CDS             3550..5217
                     /gene="polX"
                     /locus_tag="VFV93_05010"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX5164735.1"
                     /translation="MALTNAEIAEMLRANADLMEIAGENSFRVNAFRRAADAVRDHDR
                     ALTAEADLTDIPGVGAGIAAVLREILATGTFGSFEELQEQLPGSLLALLDIPGVGAKT
                     AARLYRELNVTSLTDLEEALRSGRLATMKGMGPKAQARIAEGLVFVQRRSGRTSIGMA
                     LPLAERLAGRLVDITGQPVYVAGSVRRMCVTVGNLDLVAVGEDVEAVLNAAGGLPDVA
                     RETSREDDFATFELHQGVMLRVRATTAERAGTALIEMTGSAAHLQQLRQHRELPTAAT
                     EEKCYAALGLPFIAPELREDRGELQAAREGRLPRLIEVADLRGDLHLHTTWSDGGATP
                     LEMALAAGARGYEYLGITDHSGGLGIAGGLRPERLAEQRIEIDSLRDRSPVRLLAGSE
                     VEVHRDGRLDFDDAILAGLDIVVASLHSGLRQPSVEFTDRLCSTIRNSNVDIIAHPTG
                     RLVERRQGADIDWPRVFTAALETATVLEINGDPARLDMDDEPARAAFEAGCLISIDSD
                     AHHTESLGLVRYGVGVARRAWIEPQRVVNTWPLDDLLAWLRDRTIKA"
     gene            5495..5749
                     /locus_tag="VFV93_05015"
     CDS             5495..5749
                     /locus_tag="VFV93_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164736.1"
                     /translation="MVQTDLPAWCERRSGTMPDGRYIVACYYTRDGHPVTRARAERIE
                     LVEYAPDGTPLRFQEGVVDGAFDLHTDEVSFSAAGFQFSR"
     gene            6145..7125
                     /locus_tag="VFV93_05020"
     CDS             6145..7125
                     /locus_tag="VFV93_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5164737.1"
                     /translation="MRQSSVLHDFPSSPGTVRDQPIISIRGLVKRYADFIAVNNVDLS
                     VQRNEIFGILGPNGAGKTTTLEMVEGLRTPDAGEIQVAGIDVVSDPVAVKHVIGVQLQ
                     TTALFDHLSVRELIELFAALSGGDTSSRWSSSLIGLVQLEEKTGAQVNQLSGGQQQRL
                     SIALAMVNDPTVIFLDEPTTGLDPQARRNLWDLVRQLRDSGKTVVLTTHYMEEAEYLC
                     DRVAVMDAGKIIACDTPLALIQSLGESATVRARVGRDLNAEQLASLPGATDASMVDGQ
                     LEIHTEDVPATLTALLQLSERLGVRLDNLATTSATLEDVFLSYTGRSLRE"
     gene            7122..8201
                     /locus_tag="VFV93_05025"
     CDS             7122..8201
                     /locus_tag="VFV93_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164738.1"
                     /translation="MKALRYMIVANLKMSVRNRTALFWNLAFPLIFIVLFQFLFSGDS
                     VSIDVGIVGEDTSPVSQQVVARMREAEGFNVSTGTQESELNKLDDGDRSVVVVFTPGS
                     NGSEVNAQLYWDQSDPQLGQVALSAVNTFLLDANAELATGAPVIAVQTTAVESSAIDY
                     MDFLIPGILAMSIMNSGMIGLASAFVTYRERGILRRIRATPFPLSSFILARILSQLVI
                     SVAQAAVLIVAGLLIADLDINGNLLYVFIMVTLGSLAFLSLGFVVAAFARNQETADSL
                     ANAFTFPMLFLGGVFFPVDSAPDWLQPIMRLIPLRYLADGLRNMMIHNASLPDEWLNL
                     LVMLATAAVGFALALRFFRWESNKA"
     gene            8222..8995
                     /locus_tag="VFV93_05030"
     CDS             8222..8995
                     /locus_tag="VFV93_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5164739.1"
                     /translation="MASRATTPEVRLERLSGQAVADGGFDKAILPIGATEYHGPAFPY
                     GTDSLTAETLAEAFARELGQTLVLPTLSYGVSHHHLAWPWTLSLRPDTLALVVRDIGE
                     SLLHHDLHRLLIVSAHDGNPPAATVAARILSQEHDMSVAIFSGWQGKARTALAGTREI
                     DEDHAGQSEMSMVLYAAPETARLELATHQPNQFMDYPVDLIGGFDGTVPLGYSGNAAA
                     ATADEGGAIVGALTALVVPFLRDLDSHGWKRGTWMSQIE"
     gene            9288..>9707
                     /locus_tag="VFV93_05035"
     CDS             9288..>9707
                     /locus_tag="VFV93_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX5164740.1"
                     /translation="MNRTFNNRRFSAVALALLLMLSFAAFAPAQSSAQETYVVEWGDT
                     LSGIAERYGISMEQLIALNGIDDPDLIITGQFLQMTKGSGEILQGGPQPADEVADDAV
                     DDSTELEAVAAEDDDAAETWALVNSGELPPAFDREQVR"
BASE COUNT         1621 a         3123 c         3191 g         1772 t
ORIGIN      
        1 gaacgggacg gctcctggca gggacggctg ccgggctagt aagcctgcgc agcagcgaag
       61 ggccgttgcc gaacgccccc tttgtcatcc tgaggcggca gccgaaggat ctcacctcag
      121 ccaactgatc tgccgagata agagagatcc ttcggctgcc gcctcaggat gacaaagggg
      181 gagctactgc tggatcgcct ggatgtgttc gacggccgtt gtcgcgatcg cctgaacagt
      241 cgccgtgtcg agttggtctg ggtcggtgta gatcagcgtc gttagcgcgc taccggctcg
      301 ggcgacgatc cagtagccct cgacctcgtt gccatcgttc gtgaacgaga agcggacggc
      361 gaccgactca tcgccgagcg cggggaatgc actgtcggtt tcgagcgtga cgtcggtgtc
      421 gccgcctgtg aaggtgccgc acgacgccgc ctcgacctcg aagtcgtagg ccgcctcgct
      481 gatgccgtcc ggataggcaa ccaacagttg catcagcgtc gcgctatccg gctcgaagcc
      541 ttcgtagaac tcgatcgcca cccgtgcggt cgcctcagcc gtctgtggga acagatccgc
      601 tccacagagc ccggtccagc tctccggctc cgagcggtgg gcgggctgga cctggtccca
      661 gtcttcatcg aggtcgtcga gcgtcagcag cccgcccatc agcgcttctt ccgcgtcggt
      721 gacattcttg ccggtcccga tcagcgcgtc gatccgcttc gtcgccgctt ctgctatctg
      781 gttggcgaac tcctgatcgt attcacccag cttgagcgtc gtcacgatcg ccacgtagcc
      841 ttcgagccgg acgaagacga agttgccctc cagcgtcccg gcgtcggcga catcgaacgc
      901 gacatgcagc gagaacgcct catcgccgag gtcgggcgca tcggccgggc tgagtctgaa
      961 gacggtgccg gtgtcgtcgg tccactcggt gcaggtcgcc gtatcgcgaa cgtaggccat
     1021 cgcctcaacc gcgacatcct cggggaaccg cgtgatgtct tcgaggacga agctggcgcc
     1081 gtcggctgac tggtattgaa cctcgacctc ggcgatccgc tcgacggcgc gcgggaacgg
     1141 cggcgagtcg cagacacccg gcgactggtc gagctccggc gcttcgaggc tctgctggtt
     1201 ccagtcgccc ggcagtgcgt cggccggtag cagcatgtcg atcagcgccg cctcgtcgtc
     1261 ggtgaactcg ccggcggccg ggctaccgga cgggctggcc gtcgctgcgt cgccagcagg
     1321 ctgggtagca gtaggcgacg atcctgccgt ggctgttgcg gcggccggtg tcgctgtcgc
     1381 ggatgtggtg tttgccggcg ctgatgtcgc cgtcggatcg tcgtcatcat ctccgccgca
     1441 ggcgaccagc gaggtgccaa gtagtagcgt gacaaacagt acggaaagtg ccttcagcat
     1501 gaactcgtcg tactcctgaa ctcaacagtg tctgcaagtg gacgttcaca acgaacatcg
     1561 tcgacggttt tccaatcaag taagccacga gaggcggttc agtgaccgcc gtcggcacga
     1621 atgccatgcc gccccaattc cgtgtagggc gtcgcctccg tgcgttgacg cgcgttttcg
     1681 tggacggtag gctacgcgga cgatactccg atcacaacgc agatgcgtta acgatagtgc
     1741 ctagccaggg agaaccgggt cacaatggtt agccagttgc cgcgcccgct tccggtgtcg
     1801 ccgattgccg ccgacgaggt cgaaccgacg cgccggccga ccgagcgggc gcggacgttg
     1861 ccgggccgcg tctaccacga tccagatgtg ttcgactggg agcgcgagac gtggttcagc
     1921 cgagagtggc tgttcgtcgg ccgcgaggag gacgctccgg agaccggcag cttcttccgc
     1981 tgcacggtgg ccggcgagaa cgtcgttgtc gtgcgtggca acgatgggca gttgcgcgcg
     2041 tttcacaacg tctgccgcca ccgcggtgcg agaatcgttg aggagcagag cgggcggctg
     2101 gttcgctttc agtgtccgta tcacgcctgg atctacgatc tggaggggaa cctgcggcct
     2161 ccccgccaca ccgagacgct ggaggagttc tcctgcgcgg cgttcagtct ctttccggtc
     2221 cgctgcgaaa cctgggccgg cttcgtcttc ctgaatctcg actccgccgc gccgccgctg
     2281 agcgcccacc tcggcgagtt cccgactcac ttcgaccgct tcccgctggc gagcctccgg
     2341 cgcgcgacgc ggatggagta cgaggtgcgc gccaactgga aggcgctgat ggagaactac
     2401 gcagagtgct accactgccc cggcgtccat ccgctgctca accacatcac gccctacaac
     2461 ctcggcggtt atttgcccgg cgccggcgag tgggccggga gctggatgga gctggccggg
     2521 gacttcgaga cgctctcgac cgacggctac cggcatggcc gggatgccat tcctggcatg
     2581 acgcaggacg atctcaagcg tgtctactac gcctggatct ggccgaacct gctcttcagc
     2641 ctgcatcccg actacctgat gacccatcag gtctggccgc gagacgccga gaactcgtcg
     2701 gtgatctgcg agctgtattt tcaccccgag acgatgtcgc ggccggcgtt cgacgccagc
     2761 gggccggccg agttctggga cctcaccaac cgccaggact ggcatgtctg cgaactccag
     2821 caggccggga cggcctcgcg cgtctacgtc ccgggtcgct acgcggcgat cgagcggatg
     2881 gtgcactcgt tcgatgcgcg cgttgccgac cgctacgccg gcgacggcgt ccggaccgat
     2941 gtcgcccgcc cggcgaagaa agagtgggga tcgggccgcg ctcggcgaag tgcgtgacac
     3001 cgacgagcgc tgccgcgcgg tcgttattct gcggcagcgc tcgtgcgatc gcggagtctc
     3061 ggttacagga cggccagccg ggccgcgagc gtcaagacgg cgatgatgcc catcagcgcg
     3121 atcgtgccgt tgacgcgtcc gaagcgttcg cgcagggttg gcgtcagccc gagcgccagc
     3181 atcgcggcga tgccgaggac cgggtggtac cagacgacgc tgtggccatc ggccagcagg
     3241 atgacgccga gcgcgacctg aagcgccagc aggccgtggg agatcgcgct gagccaggaa
     3301 ggcgcttcgc gcccgccgcg actcatgaaa tagatgatcc ccatgatgat gagatagacg
     3361 aggaacaacc cctccccaat cccgcgatga atgtcgaaca ctcgtcctgc tccttcccca
     3421 cctcgctccg ggcaggcgcc cggccggtat catgcgtgaa gcgtactcga cgcgatgcgt
     3481 cgcggacacg aagatgcata cgcggcgttg attctggcga acgaagacgg atacgaggcg
     3541 gagtgacgga tggctctaac gaacgctgag attgccgaga tgctgcgcgc gaatgccgac
     3601 ctgatggaga tcgccggcga gaactcgttt cgcgtcaatg ctttccggcg tgcggcagac
     3661 gccgtccgtg accacgaccg ggcactgacg gccgaggcgg atctaaccga catccccggc
     3721 gtcggcgccg gtatcgccgc cgtcttgcgg gagattctgg cgaccgggac gttcggctcg
     3781 ttcgaggagc tgcaggagca gcttcccggt tcgctgctgg cgttgctcga tatcccaggt
     3841 gtcggcgcga agacggcggc acggctctac cgcgagctga acgtgacctc gctgaccgac
     3901 ctcgaagagg ccctgcgcag cgggcggctg gcgacgatga aggggatggg gccgaaggcg
     3961 caggcccgaa tcgccgaggg gctcgtcttc gttcagcgcc gatcgggccg gacctcaatc
     4021 gggatggcgc tgccgctggc cgaacgcctc gccggccgtc ttgtcgacat taccggccag
     4081 ccggtctatg tcgccggttc ggtgcggcgg atgtgcgtga cggtcggcaa cctcgatctc
     4141 gtggcggttg gcgaagatgt cgaggccgtt ctcaacgcgg cgggtggcct gccggatgtc
     4201 gcccgcgaga cgagtcgtga ggatgatttc gccaccttcg agttgcatca gggcgtcatg
     4261 ctcagggttc gagcgacgac ggcagagcgg gctggaacgg cgctgatcga gatgaccggc
     4321 tcggccgcac acctgcaaca gctcaggcag catcgggaac taccgacggc cgcgaccgag
     4381 gagaagtgct acgccgcgct ggggctgccg ttcatcgcgc cggagttgcg tgaggaccgt
     4441 ggtgagttgc aggccgcccg cgaaggtcgc ctcccgcggc tgatcgaggt ggcggatctg
     4501 cggggcgatc tccacctgca cacgacctgg tcggacggtg gcgcgacgcc gctggagatg
     4561 gcgctggcgg ctggtgcgcg tggttacgag tatctgggaa tcaccgacca ctccggcggg
     4621 ctcgggatcg ccggtggcct gcgaccggaa cggctggccg agcagcggat cgagatcgac
     4681 agcctgcgcg accgttctcc agtccggctg ctggccgggt cagaggtcga ggtgcaccgc
     4741 gacggccggc tggatttcga cgacgcgatc ctggccggcc tcgatatcgt cgtcgcctcg
     4801 ctccactcgg ggctgcgcca gccaagcgtc gagttcaccg accggctctg ttcgacgatc
     4861 cggaacagca acgtcgacat cattgcccac ccgacgggcc ggctggtcga gcggcgtcag
     4921 ggagccgata tcgactggcc gcgggtcttt acagccgcgc tggaaacagc gaccgtcctt
     4981 gagatcaacg gcgacccggc ccggctcgac atggacgatg agccggcgcg ggcggcattc
     5041 gaagccggct gcctgatctc gatcgactca gacgcccatc acaccgaatc gctcgggctg
     5101 gtgcgctacg gcgtcggtgt tgcccgccgg gcgtggatcg agccgcaacg ggttgtcaac
     5161 acctggccgc tcgacgacct gctcgcctgg ctgcgcgatc ggacaatcaa ggcataggtg
     5221 gtgggcgcgg cgtcgtactg gtcgccagtc agcacgagtt gcaattgact cacccaaatc
     5281 acctatcgct ttttcggccg cgaaggagta ttctgtcaga accgctgccg gaggggtccg
     5341 gcggcgggag gtaatgcatc agcacgacag actcaaccca tcgtctctga tggaccgccg
     5401 cgtgctcggt gaagccgcac cgacatcgcg gtgtctttgt gtccgcacct gagattgcga
     5461 ccgtatctcg cggtcacaga gggaggtggt tcccatggtt cagacagact tgccggcgtg
     5521 gtgcgagcgc cgcagcggca cgatgcccga cgggcgctac atcgtcgcct gctattacac
     5581 gcgtgatggg catccggtta cccgtgcccg cgccgaacgg atcgagctcg ttgagtacgc
     5641 accggacgga acaccgctgc gcttccagga aggtgtcgtc gacggcgcct ttgacctgca
     5701 taccgacgag gtttccttct cggccgccgg cttccagttc agccgttgat ggccgactgg
     5761 acgcttgtcc gagcactcct tgtcgccggc tccgacgacc ggcgtcttac cggctgatat
     5821 ccttcccgcc agccggcagg gactcctgtc cctattgacg aactccaaca tcccctccaa
     5881 cagcatcaac cactatccat ctcccttgcg gctgatctca ttgatgttcc gacgcctgat
     5941 agttgtgcgt ttttcgccat cgacgtcctg aatgttcaac aggcgtagaa tgaagtgttt
     6001 gtgccgcgtg cggagcgacg gatgttcgcg gccgcgtggc agtacgggta ccgaattctc
     6061 acgcccacgc aaccggcgtc gccggttgcg tgggcgacag gatcaaggtg gcgccagcca
     6121 tctggacagg aatcgttacg gcctatgcgt cagtcctctg tgttgcacga cttcccctcc
     6181 tcgccaggaa cggtacgtga tcagccgatc atctcgattc gcgggcttgt gaaacgctac
     6241 gcggatttca tcgccgtcaa caacgtcgac ctctccgtcc agcgcaatga gatcttcggc
     6301 atcctcggcc cgaacggcgc cggcaagacg acgacgttgg agatggtcga ggggctacgg
     6361 acgccggatg ccggcgaaat ccaggtcgcc ggcatcgacg tcgtcagcga tccggtagcc
     6421 gtcaagcacg tcatcggggt ccagttgcag acgacggcgc tgttcgacca cctctctgtc
     6481 cgtgagctga tagagctgtt cgcggccctc tccggcgggg acacatcgtc gcgctggtcc
     6541 tcatctctga tcggccttgt ccagttggag gagaagaccg gcgcgcaggt caaccagctc
     6601 tccggcggtc agcagcagcg tctctcgatc gcattggcga tggtgaacga cccgactgtc
     6661 atcttcctgg acgagccgac gaccggcctc gacccgcagg ctcgtcggaa cctctgggac
     6721 ctcgttcgtc aactgcgcga ttcgggcaag acggtggtgc tgactactca ctacatggag
     6781 gaagccgagt acctctgcga ccgggtggcc gtcatggacg ccggcaagat catcgcctgc
     6841 gacaccccgc tggcactgat ccagtcgttg ggcgagagcg caacggttcg ggcgcgggta
     6901 gggcgcgatc tgaacgccga gcaactcgcg tcgctcccgg gcgcgaccga cgcgagcatg
     6961 gttgacgggc agcttgagat tcacaccgag gacgttccgg cgacgctgac ggcgctgctg
     7021 caactctcag agcggctcgg cgtgcggctt gacaacctcg cgacgaccag tgcgacgctt
     7081 gaagatgtct tcctctccta caccggacgg agtctgcgcg aatgaaagcg ctgcgctaca
     7141 tgatcgtggc caacctgaag atgagcgtcc gtaaccggac cgccctcttc tggaacctgg
     7201 ccttcccact gatcttcatc gtcctgttcc agttcctctt ttccggcgac agcgtctcga
     7261 tcgatgtcgg catcgtcggt gaagacacct cgccggtctc acagcaggtt gtcgcgcgaa
     7321 tgcgggaggc cgaggggttc aacgtctcga ccggcacgca ggagtcggaa ctgaacaagc
     7381 tcgacgatgg cgaccgctcg gtggtcgtcg tcttcacgcc cggcagcaac gggagcgagg
     7441 tcaacgccca gctctactgg gaccagagcg acccccaact cggtcaggtg gcgctcagcg
     7501 ccgtcaacac cttcctgctg gacgccaacg cggagttggc gaccggcgcg cccgtcatcg
     7561 ccgttcagac gacggcggtc gagtcgtccg cgatcgacta catggacttc ctgatccccg
     7621 gaattctggc gatgtcgatc atgaactccg gcatgatcgg gttggcgtcg gcgttcgtga
     7681 cctatcgcga gcgcggcatc ctgcgccgga ttcgcgcgac gccgttcccg ctctcgtcgt
     7741 tcattctggc gcgcatcctc agccagctcg tcatcagcgt cgcccaggca gctgtgctga
     7801 tcgtcgccgg gctgctgatc gccgacctgg acatcaacgg caacctgctc tacgtcttca
     7861 tcatggtcac gctcggctcg ctggcgttcc tctcgctggg gttcgtcgtg gcggccttcg
     7921 cccgcaatca ggagacggcc gattcgctgg ccaatgcctt cacgttcccg atgcttttcc
     7981 tcggcggggt cttcttcccg gtcgactcgg cgcccgactg gctgcagccg atcatgcggc
     8041 tgatcccgct gcgctatctt gctgatggcc tgcgcaatat gatgatccat aacgcctcgc
     8101 tgccggatga gtggctgaac ctgctcgtca tgctggcgac ggcggcggtc ggattcgcgc
     8161 tggcgttacg cttcttccgc tgggagtcca acaaggcgta gctgagagac aggggtgaac
     8221 gatggcatca cgagcgacga cgccggaggt ccggctggaa cggttgagtg ggcaggccgt
     8281 tgctgacggc gggttcgaca aggcgatcct gccaatcggc gcaactgagt accacggccc
     8341 ggcctttccg tacgggaccg actcgctgac agccgaaacg ctggccgagg cgttcgcccg
     8401 tgagttgggg cagacgctgg tcctgccgac gctctcttac ggggtcagcc accatcacct
     8461 cgcgtggccg tggacgctct cgctgcggcc tgacacgctg gcgctcgtcg tgcgcgacat
     8521 cggcgagtca ttgctgcacc acgatctcca ccggctgctg atcgtctcgg cccacgacgg
     8581 caacccgccc gcggcgaccg ttgcggcccg gatcctgtcg caggagcacg acatgagcgt
     8641 tgccatcttc tccggttggc agggcaaggc gcggacggcg ctggccggta cgcgggagat
     8701 cgacgaagat cacgccggcc agtcggagat gtcgatggtg ctctacgccg caccggaaac
     8761 ggcccggctc gaactggcga cgcatcagcc caaccagttc atggactacc ccgtcgacct
     8821 gatcggcggc ttcgacggaa ccgtgccgct cggctactcc ggcaacgctg ccgccgcgac
     8881 tgccgatgag ggcggggcca tcgtcggtgc gctaaccgcg cttgtcgtcc cctttctccg
     8941 cgatctggac agccacggct ggaaacgcgg cacctggatg agccagatcg agtaacgcat
     9001 cgaagtaatc acctcgtcat tcgagcatcc gccggtaagg gcgagtcggt gcctcgcccc
     9061 taccggtggt ggcattcatt ccctgcccca catcagaaca cggccagttc ccaccaaggt
     9121 gtacgtactg tggtatggtc cccgcctgcc ggttgagccg gcgttcgtgg gtcggtgctc
     9181 cacgtctgga gcagtgtctc gttgtgacaa tgagaccaac gttgacttcc taagcggggt
     9241 gtaggtacgc tgtgtacgta cggctttgga ggtctggagg aatgggaatg aatcgaacgt
     9301 tcaacaaccg ccgcttttct gcggttgcgc tggcgttgct gttgatgctg tcgttcgccg
     9361 cctttgcgcc ggcacagtcc agcgcgcagg aaacctatgt cgtcgagtgg ggcgatacgc
     9421 tgagcgggat cgccgagcgc tatggcatct caatggaaca gctcatcgcg ctgaacggga
     9481 ttgacgatcc tgacctgatt atcaccggtc agtttctgca gatgacaaag ggatccggtg
     9541 agatcctgca aggcggtccg cagccagccg atgaagtggc tgatgacgcc gtcgacgatt
     9601 cgaccgagct tgaagcagtc gctgcggaag atgacgatgc ggcagagacg tgggcactgg
     9661 tgaactcggg tgagttgccg ccggccttcg atcgcgagca ggttcgc
//
LOCUS       DASUGJ010000229         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1562216, whole genome shotgun sequence.
ACCESSION   DASUGJ010000229 DASUGJ010000000
VERSION     DASUGJ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562216"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VFV93_05040"
     CDS             <1..1008
                     /locus_tag="VFV93_05040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5164741.1"
                     /translation="VYEQTLDVGPFALRGTPPPIDGVGAGYCPRRTVLDQILVNAAVD
                     AGAELREHFTVKDLLFDGDRVVGIRGHARRGTLVVERAKIVIGADGLHSLVARAAGAA
                     VYNDRPSLACAYYSYWSGAPLSGAEIYPRADRAIITAPTNDGQAVVIAYWPAAMFHEV
                     RADIEGHFMRALDLAPGLAERVRGGTRTERFRGTVDLPNFYRKPFGPGWALVGDAGYH
                     KDPITAQGISDAFRDAELLADAIDDGLAGRKSLADALAGYEARRNEATAAIYEMTVQM
                     AMLQLPTAEQQQLFAALRHNQDASDRFIGTLAGSVPLEAFFAPENIGQIIGAAEPIQA
                     A"
     gene            1036..1560
                     /locus_tag="VFV93_05045"
     CDS             1036..1560
                     /locus_tag="VFV93_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5164742.1"
                     /translation="MSTTIGSNQMTGDLTPLTVGDPQLIAHPVAQELLQATIPAHLGY
                     VAPDGAPRVVPLLFHWTGSEVVVTSWPDDPKVAAIQATPTVSVTIDTSAFPFRVLSLR
                     GEATVTIVDGVAPECLPTFSRYFGPDEGRGWVERMRMMSDQQARIAIRPDWVNVVDFQ
                     TRFPSGMTKRMRQV"
     gene            complement(1567..1971)
                     /locus_tag="VFV93_05050"
     CDS             complement(1567..1971)
                     /locus_tag="VFV93_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX5164743.1"
                     /translation="MPAPRWVARANRYGLNPIFRRLAPHLPGFGVIVHRGRKSGREYR
                     TPVNVFRRTGGFVVAMTYGREAEWVRNVLASGGCTLVTRGRSFTLSNPRLFHDEDRRA
                     VPAPVRVMLGFMNVNDFLDLTLVETGQREAEG"
     gene            2175..3077
                     /locus_tag="VFV93_05055"
     CDS             2175..3077
                     /locus_tag="VFV93_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HEX5164744.1"
                     /translation="MPITTVGLLSPGDMGHSIGAVLHNHGLRVLTCLTGRSERSRALA
                     AEAGIEDVPSLEELVEEADMFLSVLVPAVALETATKVANALRTTGTNLLYIDCNAIAP
                     RTVREVGEVVSAAGGRVADIGIVGAPPRRPGTRFYASGPGASEAAGLADYGLDVRVIG
                     AEMGQASGLKMCYGALTKGLQALGVELLVAARALGLEEALRAEQQHSVPDVLSWLERS
                     VPTMPPKAYRWVGEMEEIATTFADVGLTPLILQGAADLYRFVADTPIGQESPENRDQS
                     RGLDGVVAALADALATPATAGSAD"
     gene            complement(3106..3363)
                     /locus_tag="VFV93_05060"
     CDS             complement(3106..3363)
                     /locus_tag="VFV93_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164745.1"
                     /translation="MAGNHKRHDVDDGQHHRRGTAKTRTAQDCGETALARATATASPV
                     STRAALAANGEAAHGASEAVFVDDVVTEARVYITTIRNLLA"
     gene            complement(3338..>3797)
                     /locus_tag="VFV93_05065"
     CDS             complement(3338..>3797)
                     /locus_tag="VFV93_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5164746.1"
                     /translation="LGREPDLNEIEPATRRAWETGRRITAADYLLAVDDIQQFSRVLA
                     QFLTGVDLWLTPTLGEPPIPVGTLSDTFTAYPAWVANVTGNPAMSVPLYWDADGLPIG
                     VSFLARFGDEATLIRLASQLEAARPWAGRTPPIFAVEEQLSYDGRQPQTP"
BASE COUNT          649 a         1319 c         1243 g          586 t
ORIGIN      
        1 gtctacgagc agacgctcga cgtcggcccg ttcgcgctgc gcggtacgcc cccgccgatc
       61 gacggcgtcg gagccggcta ctgcccgcgt cgaaccgtcc tcgaccagat cctcgtcaac
      121 gcggccgtcg atgccggagc cgagctgcgc gagcacttca ccgtcaagga tctgctcttc
      181 gacggcgacc gcgtcgtcgg cattcgcggc catgcgcggc gcgggacact ggtcgtcgag
      241 cgggccaaaa tcgtcatcgg cgccgacggg ctgcactcgc tggtcgcccg tgccgccggt
      301 gctgccgtct acaacgaccg cccgtcgctg gcctgcgcct actacagcta ctggtcggga
      361 gcgcccctca gcggcgctga gatctacccg cgggccgacc gcgccatcat caccgcgccg
      421 accaacgacg gccaggcggt cgtcatcgcc tactggcccg ccgccatgtt ccatgaggtg
      481 cgcgccgaca tcgaaggcca cttcatgcgg gcgctcgacc tggcacccgg cctcgccgag
      541 cgtgttcgcg gcgggacccg caccgagcgc ttccggggaa cggttgatct cccgaacttc
      601 taccgcaagc cattcggacc cggctgggcg ctggtcggcg acgccggtta ccacaaggac
      661 ccgatcacag cccagggcat cagcgacgca ttccgcgacg ccgagttgct ggccgacgcg
      721 atcgatgacg gtctcgccgg tcggaagtcc ctcgcggatg ccctcgctgg ctacgaggcg
      781 cggcgcaacg aggcgacggc cgcgatctac gagatgacgg tgcagatggc gatgctgcaa
      841 ctgccaaccg ccgaacagca gcagctcttc gccgccctgc gccacaacca ggacgccagt
      901 gaccgcttca tcggcacgct ggccgggtcg gtcccgctcg aggcgttctt cgcgccggag
      961 aacatcggcc agatcatcgg tgccgccgaa ccgatccagg ccgcgtagtt gaaacaccca
     1021 gatcggagca acccgatgtc aaccacaatc ggttccaacc agatgacagg tgacctgacg
     1081 cccctgacgg tcggcgaccc gcaactgatc gcccaccccg tcgcgcagga gcttctccag
     1141 gcgaccatcc ctgcccatct cggctacgtc gcgccggacg gcgcaccacg cgtcgtgccg
     1201 ctcctcttcc actggaccgg ctccgaggtc gtggtgacgt cgtggcccga cgatcccaaa
     1261 gtcgccgcga tccaggcgac cccaacggtc tcggtgacga tcgacaccag tgcgttcccg
     1321 ttcagggtgc tctcgctccg tggcgaggcg accgtgacga tcgtcgatgg cgtcgcgccc
     1381 gagtgtctgc cgaccttctc gcgctacttc ggccccgacg agggccgggg ctgggtcgag
     1441 cggatgcgga tgatgagcga ccagcaggct cgaatcgcaa tccgccccga ctgggtcaac
     1501 gtcgtcgatt tccagacgcg cttcccgagc ggaatgacga agcgcatgcg ccaggtctga
     1561 cggacgttac ccctcggcct cacgctggcc agtctcaacc agcgtgaggt cgaggaagtc
     1621 gttgacgttc atgaagccaa gcatgacacg aacgggcgcc ggaacagcgc gacggtcctc
     1681 atcgtgaaac agacgcgggt tggagagggt aaaggagcgc ccgcgcgtca ccagcgtaca
     1741 gccgccgctg gcgaggacgt tgcggaccca ctccgcttcg cgcccatacg tcatcgcgac
     1801 gacaaagccg cccgtgcgcc gaaagacatt gaccggcgtc cggtactccc tgcctgactt
     1861 ccgcccccga tggacaatga cgccgaagcc cggcagatgt ggcgccaggc ggcggaagat
     1921 cgggttcagc ccgtaccgat tcgcgcgagc cacccaacgc ggagccggca tcgggaccac
     1981 ctccagacac gaacgctgcg tcacttcgaa gcgattcgtt cggcgatccc atcttacgct
     2041 actcagatgt atcttcatgg ggtgtggcgt gccgaaatgg ccgaggacgg ccggattcac
     2101 agtcgggccg agcgctggag cgtatcatga aagtgagacg cccgttccat tggggcatca
     2161 cgaaaggatc caccatgccc atcacgacgg tcggcctgct cagccctggc gacatgggac
     2221 actcgatcgg cgcggtgctg cacaaccacg gcctgcgcgt gctgacctgc ctgaccgggc
     2281 ggagcgagcg ctcgcgggcg ctggccgccg aagcgggcat cgaggacgtt cccagcctcg
     2341 aagaactggt cgaagaggcg gacatgttcc tctccgtcct ggtgccggcc gtcgcgttgg
     2401 agacggcgac aaaggtggcg aacgcgctac gcacgaccgg gacgaatctg ctgtacatcg
     2461 actgcaacgc gatcgcgccg aggacggtcc gggaggtcgg cgaggtcgtc agcgcggccg
     2521 gcgggcgcgt ggccgacatc ggcattgtcg gtgcgccacc gcgccgcccc ggtacgcgtt
     2581 tctacgcctc cggacccggc gcgagcgagg cagcggggct ggccgactat gggctggacg
     2641 tgcgcgtcat cggcgctgag atgggccagg cgtccggcct gaagatgtgt tacggcgcgc
     2701 tgaccaaggg gctccaggcg ctcggcgtcg agctgctggt cgccgcccgc gccctcggac
     2761 tcgaggaggc gctgcgtgcc gagcagcagc acagcgttcc cgatgtcctc agctggctgg
     2821 agcgatccgt cccgacgatg ccgcccaaag cctaccgctg ggtcggcgag atggaggaga
     2881 tcgcgacgac cttcgccgat gtcggcctga ccccgctcat cctgcaaggc gccgccgacc
     2941 tctaccgctt cgtcgctgac accccgatcg gtcaggagtc tccggaaaac cgcgaccaga
     3001 gccgggggct cgacggcgtc gtcgccgcgc tggcagatgc gctggcaacg ccggcaacgg
     3061 caggatcggc ggactagcac ccggcgttcg ggcactgcga gaggctcacg ccagcaggtt
     3121 gcgaatggtc gtgatataca cccgtgcctc ggtgacgacg tcgtccacga agacagcctc
     3181 gctcgcgccg tgagcggcct cgccatttgc cgccaaagcc gcccgtgtcg ataccggcga
     3241 agcggttgcg gtggcgcgtg cgagtgccgt ctctccacaa tcctgcgcgg tacgcgtttt
     3301 tgccgttcct cgtcgatgat gctgaccatc gtcgacgtca tggcgtttgt ggttgcctgc
     3361 catcgtagga cagttgttcc tcaacagcga agatcggcgg agtgcgacct gcccacggac
     3421 gggcagcctc aagttgggac gccagccgga tgagcgtcgc ctcgtcaccg aagcgagcga
     3481 gaaagctgac accgatcgga agcccatcgg catcccagta gagcggcacc gacatggccg
     3541 ggttgccggt cacgttcgcc acccaggcag gataggccgt gaacgtatca ctcagtgttc
     3601 cgaccggaat cggcggctcg ccgagtgtcg gcgtcagcca gagatcgacg ccagtcagga
     3661 actgcgcgag gacgcgactg aactgctgga tatcgtcgac tgcaagaaga taatcggcgg
     3721 cggtgatcct ccgtccggtc tcccaggccc gacgggtcgc cggctcaatc tcgttcagat
     3781 ccggttcgcg ccccagc
//
LOCUS       DASUGJ010000230         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_310870, whole genome shotgun sequence.
ACCESSION   DASUGJ010000230 DASUGJ010000000
VERSION     DASUGJ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310870"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..>2640
                     /locus_tag="VFV93_05070"
     CDS             256..>2640
                     /locus_tag="VFV93_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX5164747.1"
                     /translation="MDRAALLQQLQFRSIGPTRGGRVVAVAGHPSQPATFYFGSTGGG
                     VWKTTDAGQLWENVSDGYFKRASVGGVAVAASDPNVIYVGMGESTIRGNVSHGDGVYK
                     STDGAKSWKHLGLEKTRNIGKVRVDPRDPERVYVAAFGHAHGPNAERGLYRSLDGGAT
                     WDNVLFISERAGVNDLSIDPRNPRILFAGSWEAERGPHFLSSGGEGSGLWRSRDGGDS
                     WERLDDKPGLPKGIKGKIGVAVSPARSDRVYAIIEHNDGGVYRTDDGGESWIRLSDNR
                     DLRQRAWYYSHIYADPQDADTVWVLNVEMWKSVDAGKTFFQVPAPHGDNHDLWIDPNN
                     PQRMILGNDGGGTITHNGGGSWSTLYNQPTGEFYHVTVDSRVPYRVYGAQQDNTTMSV
                     PSQSNYDAIIFPDWLEIGGGESGYIAVRPDNPDVIYAGSMQGYLTRLDNTAGQLRNVT
                     VWPESYMGWGAQDMKYRFNWTSPTVLSPHDPNVLLTGANVIFKTTDEGMSWEAISPDL
                     TRGDPETLGLSGGPITKDNTGAEAYGTVFTIAPSPLDANVIWSGSDDGLIHVTRDGGA
                     NWEATTPPTLPDWALISLIDASPHDPATAYVAATRYKLDDFAPYLFRTNDYGTTWTQI
                     TNGIPGDDFTRVVREDPAQQGLLYAGTETGIYVSFDDGANWQKLGGNLPVVPIHDLIV
                     HNDGLVIGTHGRSFWILDDLTPIRELAGDAASKAAHLFQPRDTIRYGRLHGFGHSPVE
                     GTNFHFAGGFIPTYRYEIDEAGNPKITFIDAGNNPPDGVAIAYTLAEKPTGDISL"
BASE COUNT          474 a          882 c          871 g          413 t
ORIGIN      
        1 gtccggaacg agcgggttga tgtttgcccc tgatcattgg tggagtggac atacagcgcc
       61 tcggcataca gcacaaacgt cacgacgccg gccgggtgcc agtcggacag ctgacctcca
      121 ggtaccttca atcacgttcc cgcttgtcca gacaggcccg tacggatgcg ccagcgcgct
      181 cgttcatcgt agactctggc gcaacgcagg tggcccgtct ggtgggttgc cggttgggag
      241 gacggggagc gacgagtgga tcgagcagcg ttgttgcagc aactacagtt tcggtcgatt
      301 gggccgaccc ggggtgggcg tgtcgtggcg gtggcggggc atcccagcca gccggcgacg
      361 ttctatttcg gctcgaccgg tggtggggtc tggaagacga ccgacgccgg ccagctctgg
      421 gaaaacgtct cggacgggta tttcaagcgc gcgtcggtcg gtggggtcgc tgtcgcggct
      481 tcggacccga acgtgatcta cgtcgggatg ggcgagagca cgatccgtgg caacgtcagc
      541 cacggtgacg gtgtctacaa gtcgaccgac ggtgccaaga gctggaagca cctcgggctg
      601 gagaagacgc gcaacatcgg caaggtgcgg gtcgatccgc gcgatccgga gcgggtctac
      661 gtggcagcct tcggccatgc ccacgggccg aacgccgagc gagggctgta ccgctcgctg
      721 gatggcggtg cgacctggga caacgtgctg ttcatctccg agcgggccgg cgtcaacgat
      781 ctctcaatcg atccgcgcaa cccgaggatc ctcttcgccg gttcgtggga ggccgagcgc
      841 gggccgcact tcctttcctc cggcggcgag ggtagcggcc tctggcgctc gcgcgatggc
      901 ggcgattcgt gggagcggct ggacgacaag cccggcctgc caaaggggat caaaggcaag
      961 atcggcgtgg ccgtttcgcc ggcccggtct gaccgcgtct acgcgatcat cgagcacaat
     1021 gacggcggcg tctaccgcac cgacgacggc ggcgagtcgt ggatccggct ctcggataac
     1081 cgcgacctgc gccagcgcgc ctggtactac tcgcacatct acgccgaccc gcaggacgcc
     1141 gacacggtct gggtgctgaa tgtcgagatg tggaagtcgg tcgatgccgg caagaccttc
     1201 ttccaggtcc cggcaccgca tggcgacaac cacgatctct ggatcgaccc gaacaacccg
     1261 cagcggatga tcctcggcaa cgacggcggc gggacgatca cccacaacgg tggtgggagc
     1321 tggtcgacgc tctacaacca gccgaccggc gagttctatc acgtgaccgt cgatagccgg
     1381 gtgccgtatc gcgtctacgg cgctcagcag gacaacacga cgatgtcggt cccgagccag
     1441 tcgaactacg acgcgatcat cttccccgac tggctggaga tcggtggtgg cgagtcgggc
     1501 tacatcgccg tccggcccga caacccggat gtcatctatg ccggcagcat gcagggctac
     1561 ctgacccgcc tggacaacac ggccggccag ctgcgcaacg tgaccgtctg gcccgagtcg
     1621 tacatgggct ggggcgcgca ggacatgaag taccgcttca actggaccag cccgaccgtc
     1681 ctctcgccgc atgacccgaa cgtgctgctg accggcgcga acgtgatctt caagacgacc
     1741 gatgagggga tgagctggga ggcgatctcg cccgacctga ctcgtggcga ccctgagacg
     1801 ctgggcctct cgggcgggcc gatcacgaag gacaacaccg gcgccgaggc gtacgggacc
     1861 gtcttcacga tcgcgccatc gccgctggac gccaacgtga tctggtccgg ttcggacgac
     1921 gggctgatcc acgtcacccg cgacggcggc gccaactggg aggcgaccac cccgccgacg
     1981 ctgcccgact gggcgctgat ctcgctgatc gacgccagcc cgcacgaccc ggccaccgcc
     2041 tacgtcgccg cgacccgcta caagctggac gacttcgcgc cctacctgtt cagaaccaac
     2101 gactacggca cgacctggac acagatcacc aacggcatcc ccggcgacga cttcacccgc
     2161 gtcgtccgcg aggacccggc gcagcagggc ttgctctacg ccggaacgga gacgggcatc
     2221 tacgtctcgt tcgacgatgg cgcgaactgg cagaagctgg gcgggaatct ccccgtcgtc
     2281 ccgatccacg atctgatcgt ccacaacgac ggcctcgtga tcggcacgca tggccgcagc
     2341 ttctggattc tggatgacct gacgccgatt cgggagctgg cgggcgatgc ggctagcaag
     2401 gctgcccacc tgttccagcc gcgcgacacg atccgctacg gacggctgca cggcttcggc
     2461 cacagcccgg tcgaggggac caacttccac ttcgccggtg gcttcatccc gacctaccgc
     2521 tacgagatcg acgaggccgg caacccgaag atcaccttca tcgacgccgg caacaacccg
     2581 cccgatggcg tcgcgatcgc ctacacgctg gccgagaagc cgaccggcga catctcgctg
//
LOCUS       DASUGJ010000231         3380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_310934, whole genome shotgun sequence.
ACCESSION   DASUGJ010000231 DASUGJ010000000
VERSION     DASUGJ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310934"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..2890
                     /locus_tag="VFV93_05075"
     CDS             173..2890
                     /locus_tag="VFV93_05075"
                     /inference="COORDINATES: protein motif:HMM:NF039776.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HEX5164748.1"
                     /translation="MDAAGNFLISWQSQHQDGFSWGIYGREYDASGNTLIGEFPINHR
                     VEGPQTSPAVASNASGQTLVAWLGNSSTHEPSVLAHRYAIPNVEPDFSVGGELSLTTY
                     AGLEEMPPAAAMNAAREAVVAWVSYAEDGSGLGVFAQLLDKYGAPIGDRIAVNSFTAG
                     NQGAPAVARAPDGQFVIVWQSEDQEESGDGYGIYAQRYSATGVPEGLTFLVNTTVAGD
                     QKAPAVAMGTDGRFVIAWESVSDDGSLDIMAQRYLPDGQADGGEFRVNNLTGLDQKDP
                     AIAMNAAGQFVIAWVSDHPALEPEAVDTEKSIFVQWYDSNGQATGDEVLVHKYVKDAQ
                     EAPAVGIDGTGRFVVAWQSINQDGNSWGVFARRFLADKTPVERREFVVNETRMGPQRY
                     VGLGMDEYGRFVITWQSNTRAELADGGSGGGGGGQNPNTPEGSSWDLFGRQYGFDGQR
                     EGGELPVNQWQMGPQIRPVVAQAAGGDFGIFWIGQGPDHIEGVHGRLYQNLFDLGDAA
                     DPAYPTLLSSNGARHLPWSDLYLGSSVDVEVEGQPTADATGDDLDVNGDDEDGVVLPI
                     VMIPGMAATATVTASVAGKLDAWIDFNQNGVFEPAERIASSLDVVAGENAVTLVVPPA
                     ALPGTSTARFRISTVGGLAPDGVAVDGEVEDHRVEISAQNSARLVDDPANPGQKVLVV
                     VGSTGKNFLTIAPDAAGNLMVREGKKVKSSTVLGVFSSAEVSRIVAYGLANNDTIRVD
                     SRISTPSELHGNAGKDALYGGAGDDLLYGDEGNDTLNGDSGNDVLVGGLDKDKLTGGH
                     GRDLLIGGAGADGLDGGDDDDILIGGSTTHDSQPSALSAIMAVWRSAAGFLERIASLG
                     GLLNSSTVIGDGIREKVTGGRGRDWMIDFESQDQFKDFDSNPTTGDHQN"
     gene            complement(2897..>3380)
                     /locus_tag="VFV93_05080"
     CDS             complement(2897..>3380)
                     /locus_tag="VFV93_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164749.1"
                     /translation="AIRDQLPRWSGTPLDRDNVGGYLEEQTHVCSLAPTEKFQAVLVI
                     DQGDREDIAVGTEVRLKIEELPDVVLQGGVTDLSHRHFEFVPPALSNKYGGPLSTVSD
                     PRGREKATSHTYEATVALDVDPRLLRDGMRGEARLIVARRSAAGWLWRWVRTTFKFRL
                     "
BASE COUNT          633 a         1054 c         1150 g          543 t
ORIGIN      
        1 cgtggcgatg gatgcggacg gcgacgccgt cgttgtctgg caggtggaag gacaggaagg
       61 gagtggatcg gacgtctacg gccggcgcat gaactccagc gggacgctgc tcggcgacga
      121 tttccgcgtc aatgaaacga cccagcgtcc gcagcggctc ccggcggtcg cgatggacgc
      181 cgccggcaac ttcctgatct cctggcagag ccagcatcag gacgggttca gttgggggat
      241 ttacggccgc gagtacgatg caagcggaaa cacgctcatc ggcgaattcc cgatcaacca
      301 tcgggtggaa ggtccgcaga ccagtccggc cgtcgccagc aacgcctcgg ggcagacgct
      361 ggtcgcctgg ctcggcaaca gttcgacgca cgagccgtcg gtcctcgccc accgctacgc
      421 gatacccaac gtggagccgg acttctcggt cggcggcgag ctatcgttga ccacgtacgc
      481 ggggctggaa gaaatgccgc cggcggccgc aatgaacgcc gcccgcgaag cggtggtggc
      541 ctgggtcagc tatgccgaag acggcagtgg attgggagtg tttgcgcagc ttctcgataa
      601 atacggcgca ccgattggtg atcggattgc agtcaactcg tttacggccg ggaaccaggg
      661 cgcgccggcc gtggcgcggg cgcccgacgg gcagttcgtc attgtctggc agagcgagga
      721 ccaggaggag agcggcgacg gttacggcat ctatgcccag cgttattcgg ccaccggcgt
      781 cccggaagga ctgacgttcc tggtcaacac gaccgtcgcg ggtgaccaga aggcgcccgc
      841 cgtggcgatg gggaccgatg ggcgatttgt catcgcctgg gagagcgtca gtgacgacgg
      901 cagtctcgac atcatggccc agcgctacct gcccgacggt caggccgacg gcggcgagtt
      961 ccgcgtgaac aacttgaccg gtctggatca gaaggatccg gcgattgcca tgaatgctgc
     1021 cgggcagttc gtcatcgctt gggtcagcga ccatccggcc ctggaaccgg aagccgtcga
     1081 tacggaaaag agcatcttcg tccagtggta cgacagcaac ggacaggcca ccggcgatga
     1141 agtgctggtc cacaagtacg tcaaggacgc tcaggaagcg ccggcggtcg gcatcgacgg
     1201 cacgggacgg ttcgtcgtgg cgtggcagag catcaaccag gacgggaact cctggggtgt
     1261 cttcgcgcgg cggttcctgg ccgacaagac cccggtcgag cgacgtgagt tcgtcgtcaa
     1321 cgaaacgcgg atggggccgc agcgatacgt cggtctcggc atggacgagt acggccgctt
     1381 tgtcatcacg tggcagtcga acacccgcgc cgagttggcc gatggaggca gcggcggtgg
     1441 gggcggcgga cagaacccga acactccgga agggagcagt tgggacctgt tcggccgcca
     1501 gtatgggttc gacggtcagc gggaaggggg cgaactgccc gttaatcagt ggcagatggg
     1561 gccgcagatt cgtcccgtcg tcgcccaggc agccggcggt gacttcggca tcttctggat
     1621 cgggcagggg cccgaccata tcgaaggggt ccacggccga ctctaccaga acctgttcga
     1681 tttgggtgac gcggccgatc cggcgtatcc gaccctgctg tcgagcaacg gggcacggca
     1741 cctgccgtgg tccgacctct atctgggcag cagtgtcgac gtggaagtgg aaggccaacc
     1801 gacggccgat gcaaccggcg acgatttgga cgtgaacggc gacgacgaag atggcgtcgt
     1861 cctgccaatc gtgatgatcc cgggcatggc cgccacggcc actgtcacgg cgtcggttgc
     1921 cggcaagctc gatgcctgga tcgacttcaa ccagaacggc gtgttcgagc cggccgagcg
     1981 catcgcctcc agcctggatg tcgttgccgg tgagaatgcc gtaacgctcg tggttccgcc
     2041 cgccgcactt ccgggaacga gcactgcccg cttccgcatc agcactgtcg gtggtctcgc
     2101 gccggacggc gtcgccgtcg atggcgaagt ggaggatcat cgcgtggaga tcagcgcgca
     2161 gaactccgcc cggctggtcg acgatcccgc gaaccccgga cagaaggtcc tggtcgtcgt
     2221 ggggtccacc ggcaagaact tcctgaccat cgcacccgac gcagccggca acctgatggt
     2281 ccgcgaaggg aagaaggtga aatcgagcac cgtgctgggc gtcttctcgt cggctgaagt
     2341 gagccggatt gtggcctatg gtctcgccaa caacgacacg atccgcgtgg attcgcggat
     2401 ctcgacgccc tccgaactgc acggcaacgc cggtaaggat gcgctctacg gcggtgccgg
     2461 cgacgacctg ctctacggcg acgaaggcaa cgacacgctc aacggagaca gcggcaacga
     2521 cgttctggtg ggcggcctcg acaaggacaa gctgaccggc ggccacggtc gcgacctgct
     2581 gatcggcggc gcgggggccg acggtcttga tggtggcgac gacgacgaca tcctgatcgg
     2641 cggcagcacc acgcacgaca gtcagccgtc ggccctgtcg gccatcatgg ccgtctggcg
     2701 gtccgcagca ggcttcctgg agcggatcgc cagcctcggc ggattgctga attccagcac
     2761 cgtcatcggc gacgggattc gcgagaaggt gaccggcggc cgaggccgcg actggatgat
     2821 cgacttcgag tcgcaagacc agttcaagga tttcgacagc aacccgacga cgggcgatca
     2881 tcaaaactga tggcggttac agccggaact tgaacgtcgt ccgcacccac cgccagagcc
     2941 agccggcggc agagcgccgg gcgacgatca gtcgcgcctc cccccgcatg ccgtcgcgaa
     3001 gcaaccgggg atcgacgtcc agcgcaacgg ttgcctcgta tgtatggctt gtcgcttttt
     3061 cccgtccgcg cggatcagaa accgtggaga gcgggccgcc gtatttgttc gacaaggccg
     3121 gcggcacgaa ctcaaaatgc cggtgcgaaa gatcggtcac acccccctgc agcacgacgt
     3181 ctggaagttc ctcgatcttg agccgcactt ccgtcccgac cgcaatgtcc tcccgatccc
     3241 cctggtcaat caccagcacc gcctggaatt tctccgttgg agcgaggctg cagacgtgcg
     3301 tctgttcctc gaggtaaccg ccgacgttat cgcggtccag tggcgttcca gaccagcgcg
     3361 gcaactgatc acggatggca
//
LOCUS       DASUGJ010000232         5779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_867341, whole genome shotgun sequence.
ACCESSION   DASUGJ010000232 DASUGJ010000000
VERSION     DASUGJ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867341"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VFV93_05085"
     CDS             complement(<1..1260)
                     /locus_tag="VFV93_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5164750.1"
                     /translation="MKKTSPEPDLAPAAVELLALSEAEAARRAARGESNAAPVATSRS
                     YGRVIFQNTVSFINILLFSIVIFLAILGLYSDAAMTAVLVVANVLVGTVQEIRAKRKL
                     DQIALLTRPTATVIRDKKERTIDPAQVVKGDLLVLKPGDQVLVDGVVVQDGSLSVDES
                     LLTGESDLVQKKKGEEVYSGSFCMTGSGIYRAEKVGGESLANQMTGQARAYRNVITPL
                     QREIILIIRVMIVLMVVLGAQVADTFRSLYNDVPLTESVRAAAVIVALVPQGLIFMIT
                     VTYAMAAVRMSGKGALIQRMNAVESTSQIDILCMDKTGTLTTNNLALHALHPSGRDEP
                     ELRRLLGLYAVATPTANRTIDALRTAIPTGHRAPCREIPFSSARKWSALAFDGDDGGT
                     YVLGAPEMLRPVLQEDATRDAQIEEWAD"
     gene            1532..2719
                     /locus_tag="VFV93_05090"
     CDS             1532..2719
                     /locus_tag="VFV93_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5164751.1"
                     /translation="MITDRSRSGSGVDTCKRERRWPFRLLLGVTVLVLATGTLSAWGY
                     SQGWDRYIHYRHLPDWNRSHEKALLATLGATATANSAAATPSPRTAMISPNASPAAAS
                     SPTVTSTPTVDMAGLSDGNVTSKSIDSPTLGKPFGYRIYLPPGYDDKRFATTHYPVIF
                     LLHGAPGGKDDWIDGGLANQTADALIKTATIRPVIIVMPDGSLGDPHHDTQWGNSPVT
                     GERVEDALVDDLIPAIDATYRTIPGPDTRAIAGLSSGGYGALNIALHHPDTFHVVVSL
                     SGYFKAAQTYDGHDIWGSDQARAENSPANNVGSRDQPLHIELIDGLDEGQGVDDTKAF
                     DAQLIDAKIDHQTLFFPGGHAWEFWKDHLLEALEYCDSHLAHHASAPGGFARGGSWPA
                     ATT"
     gene            2731..5172
                     /locus_tag="VFV93_05095"
     CDS             2731..5172
                     /locus_tag="VFV93_05095"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX5164752.1"
                     /translation="MDTRHNAPASAIPAIDLNQLRRVLRHRGWRVPFTVSLLAIMVIS
                     NWAEGSLTHSVASNGLLEHVGFGVPSLRDGQFDTLLTSIPFALYPWMLATIAFLVLIG
                     VAPFEILAGWKRTAIVFFVSQTGGYLLAALLVSWPLATLGVNWGQHLANARDVGPSAG
                     AFGCLAALVWFLPERWRSLGGGALFAYLLGFLLLTHRVWDVEHLIGGLLGLGAGWLLI
                     ERRELIAGAFDALRTLPRPDARDIVAALVALAGLMNIVSALVTTASSRVASWDTDVPF
                     AILHGTRTYVTLSGFALILLGRSLSQGRRVGWVATITLLAGSVISHVVKGLDIEEASV
                     ELLLIVALVVRHNDYQARPDVVSVTRALRLSAVALAALPVYIAAGFWLMRDDFDRPLR
                     TGLAARESLARLLFTTTHQLDGASFEANWFLDSMTWIWAATLLFCSVALLRPVLRPTI
                     ETSSDRRDAIDLLHHYGGSPIGQMTTWPGNTLLLNHHRNAYLAYRLIGNVALVLGDPI
                     GAPEGRSAGIQEFVQLCHRNGWTPCFYGVGRSNLDAYSRAGLSSIQVGEDAYIDLSDF
                     HLHGRQWQDARTAINKARKMGIEFHLADLGGVDDGTLRQLHEISDSWLHSRRLPEMGF
                     TLGSLTAKPDPEAKVATAIDQRGHVHAFVTWLPIYGTCGWVIDIMRRRPDAFNGVIEF
                     LIVESALAFRTEGAAVVSLSTAPLARVARDGDAADTFEQAITRLVGLIEPFYQTRTLF
                     EFKRKFSPRWEPVYIAFPGATCLPRIGYAILRAYLPTLSIEDIRDLLAPARAGRPKPR
                     EGVPDHQSDTESTTA"
     gene            complement(5196..5702)
                     /gene="paaD"
                     /locus_tag="VFV93_05100"
     CDS             complement(5196..5702)
                     /gene="paaD"
                     /locus_tag="VFV93_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX5164753.1"
                     /translation="MTTSTAITEQQIWDALQDVPDPEIPAISVVDLGIIRSVEIDGGR
                     VSVEIMPTFIGCPAIGIMCADVEQRLRQIADEVEVRVTYTEQWTSDRITERGRENLRA
                     AGYAPPVTPAPSLGDNLFPLALHPVVPCPFCGGRHTRMENPFGPTLCRAIYYCPDCKQ
                     PFEHFKSV"
BASE COUNT         1005 a         1925 c         1791 g         1058 t
ORIGIN      
        1 gtcagcccac tcctcaatct gagcgtcgcg tgtcgcgtcc tcctggagga caggccgcag
       61 catctccggc gcaccgagga cgtaggtgcc gccatcgtca ccatcgaagg ccagcgcgct
      121 ccacttgcgc gccgacgaga acggaatctc acggcacggc gcgcggtgtc cggtcgggat
      181 cgccgtccgc agcgcatcga tcgtccggtt cgcggtcggc gtggcgacgg cgtagagccc
      241 gagcagccgc cgtagctccg gctcgtcgcg accggagggg tggagggcat gcagggcgag
      301 gttgttggtc gtcaacgtcc cggtcttgtc catgcagagg atatcgatct ggctggtcga
      361 ctcaacggcg ttcatgcgct ggatcaacgc gcccttgccg gacatccgga cggccgccat
      421 cgcgtacgtc accgtgatca tgaagatcag cccctgtggg acgagcgcga cgatgacggc
      481 ggctgcccgg actgattcgg tcagcggcac gtcgttgtag agcgagcgaa acgtgtcggc
      541 gacctgagct ccgagcacga ccatcaggac gatcatgacc cgaatgatca ggatgatctc
      601 gcgctgcaat ggcgtgatca cgttgcggta ggcccgcgcc tggccggtca tctggttggc
      661 gaggctctcg ccgccgacct tctcggcccg gtagatgccg ctgccggtca tgcagaacga
      721 gccggagtag acctcctcgc cctttttctt ctggaccaga tcggactcgc cggtcagcag
      781 cgactcatcg acgctgaggc tgccgtcctg gacgaccacg ccatcgacca gcacctgatc
      841 accgggcttg aggaccagca gatcgccctt gacgacctgg gccgggtcga tcgtgcgttc
      901 cttcttgtcg cgaatcacgg tcgccgttgg ccgcgtcagc agcgcgatct ggtcgagctt
      961 gcgcttggcg cggatctcct gaaccgtccc caccaggacg ttggcgacga ccaggacggc
     1021 cgtcatcgcg gcgtcactgt agagcccgag gatggccagg aagatgacga ttgagaagag
     1081 caggatgttg atgaacgaga cggtgttctg gaagatgacc ctgccatagg aacgactggt
     1141 cgcaaccggc gcggcgttac tctcgccccg ggcggcgcgc cgggctgcct ccgcctcgct
     1201 cagcgccagt agctcgacgg ccgccggggc aagatcaggt tccggcgatg tctttttcac
     1261 gaccggaagc ttcgctgtta tcgccgtact atctgccatc acgcgtaccg ttgcgagccg
     1321 acccacagca tcaccgtgaa cgatcccatc agggtaacag aatccgctct gtatggaaca
     1381 cgttacaccc ggcccaatgc atggatactc cagccttcag cccattttca ggtccgtttc
     1441 aagcccgctt catcgtcgcg cgggacaatt cgaacgcttg cggacccgcg tgcgggtccg
     1501 acgtttgccg gcttccgcat ggaaccacgc gatgatcaca gaccgttcgc gttcaggctc
     1561 aggcgtcgat acatgcaaac gtgagcgccg atggccgttc cggctgctcc ttggtgttac
     1621 cgttctggtg ctggccaccg ggacgctgag cgcctggggc tacagccagg gctgggaccg
     1681 ctacatccac taccgtcacc tccccgactg gaaccggtcg cacgagaaag cgttgctggc
     1741 gacactcggg gcgacggcga cggccaacag cgccgccgcg accccgtcgc cgcgcacagc
     1801 catgatcagc ccgaacgcat cgccggcagc ggcatcgtcg ccgaccgtca cctccacgcc
     1861 aacggttgac atggccggct tgagtgatgg caacgtcacg agcaagagca tcgacagtcc
     1921 gacactggga aagccgtttg gctaccgtat ctatttgccg cccggttacg acgataaacg
     1981 gttcgcgacg acacactacc cggtcatctt cctgctccac ggcgcaccag gcggaaagga
     2041 tgactggatc gacggtggac tcgccaatca gacggccgac gcattgatca agacggcgac
     2101 gatccggccg gtcattatcg tgatgccgga cggctcgctc ggcgatccac atcacgacac
     2161 gcagtggggc aactcgccag tgactggcga gcgcgtcgag gacgcgctag tggacgatct
     2221 gatcccagcc atcgacgcga cctaccgtac gatcccaggg ccggacacac gcgcgatcgc
     2281 gggcctgtca tccgggggat acggagcgct caacatcgcg ctccatcacc ccgacacatt
     2341 ccacgttgtt gtctcgctgt ccgggtactt caaggcagcg cagacctacg atggccacga
     2401 catctggggc agcgatcagg cgcgcgccga gaacagcccg gccaacaacg ttggctcgcg
     2461 cgaccagccg ctccacatcg agctgatcga cggacttgac gagggccagg gggtcgacga
     2521 caccaaggcg ttcgacgcac agctcatcga cgcaaagatc gaccatcaga cgttgttctt
     2581 tccaggcggt cacgcctggg agttctggaa ggatcatctc ctcgaagcgc ttgagtactg
     2641 cgacagccac cttgcacacc acgcgtccgc tccaggcgga ttcgcgcgtg gcggtagctg
     2701 gccggcggcc acgacatgag agggacggcg atggacacgc ggcacaacgc ccctgcatcg
     2761 gcgatccccg ccattgacct caaccagctc cgccgcgtcc ttcgccatcg tggctggcgc
     2821 gtcccattca ccgtcagcct gctcgccatc atggtgatct ccaactgggc cgaaggatcg
     2881 ctgacgcaca gcgtcgccag caacgggctg ctcgagcacg tcggcttcgg ggtgccatcg
     2941 ctccgggatg gtcaattcga cacactgctg accagcattc cattcgcgct gtatccgtgg
     3001 atgctggcga cgatcgcgtt ccttgtcctg atcggtgtcg cgccgttcga gatactggcc
     3061 ggctggaaac gtacggccat cgtcttcttc gtgtcacaga ccggtggcta cctgctcgcg
     3121 gcgctactgg tctcctggcc gctcgcgacg ctgggcgtca actggggcca gcatctggcg
     3181 aatgcccgtg atgtcggtcc ctcggctggc gcgtttggct gtctggcggc gctggtctgg
     3241 ttccttccag agcgatggcg gtctctcggc ggcggcgcgc tctttgcata cctgctggga
     3301 ttcctgctac tgacgcaccg tgtctgggat gtcgagcacc tgattggcgg gctactcggc
     3361 ctcggcgccg gatggctgct catcgaacgg cgagaattga tcgctggagc attcgacgca
     3421 ctccggacac ttccccggcc ggacgcgcgc gacatcgtcg ctgctctggt tgccctggcc
     3481 ggcctcatga acatcgtttc ggcgcttgtg acgacggcgt ctagccgcgt cgccagttgg
     3541 gacacggacg taccatttgc gatcctgcat ggcactcgca cctacgtcac actctccggc
     3601 ttcgcactga tcctgctggg ccgcagcctg agccagggcc gccgtgtcgg ctgggttgcg
     3661 acgatcaccc tgctggccgg ttccgtcatc tcccacgtcg tcaaggggct cgacatcgaa
     3721 gaagcgagtg tcgagctgct attgatcgtc gcgctcgtcg tacgtcataa cgattaccag
     3781 gcccggcctg atgtcgtttc tgtgacccgc gcactcagac tgtccgccgt ggcgctcgct
     3841 gctctaccgg tctacatcgc tgccggcttc tggctgatgc gcgatgactt cgaccgcccg
     3901 ctccgcaccg gcctcgcggc gcgcgagtcg ctcgcccgac tgctctttac aacgacccac
     3961 cagctcgacg gagcgtcgtt cgaagccaac tggttcctcg actccatgac gtggatctgg
     4021 gccgcgacgc tgctgttttg cagcgtcgcg ctgctgcgcc cggtgctgcg cccgacgatc
     4081 gagacatcca gtgaccggcg cgacgccatc gacttgctac accattacgg cggatctcca
     4141 atcggacaga tgacgacctg gcccggcaac acgctgttgc tcaaccatca ccgcaacgcc
     4201 tatctcgcct accggctgat cggcaatgtc gcgctcgtgc tgggcgatcc gatcggcgcc
     4261 cccgagggac ggagcgccgg gattcaggag tttgtccagc tctgccaccg caacggctgg
     4321 accccgtgct tctatggtgt gggacgttcc aacctcgatg cgtattctcg cgcaggactc
     4381 tcgagcatcc aggtcggcga agacgcctat atcgacctct cagatttcca tctgcatggg
     4441 cgacagtggc aggatgcacg gaccgcaatc aacaaggcgc gcaagatggg gatcgagttt
     4501 catttggccg atctcggggg agtcgacgat ggaaccctgc gtcagctcca cgagatttcg
     4561 gacagctggc tgcactcgcg tcgcctgccg gagatgggct tcacgctcgg tagcctcacc
     4621 gcgaagcccg accctgaggc caaagtcgcg accgcgatcg accagcgggg ccacgtccac
     4681 gccttcgtga cctggctgcc gatctacggc acctgtggct gggtgatcga catcatgcgt
     4741 cgccgaccag acgcattcaa cggagtcatc gagttcctga tcgtcgagtc agcgctggca
     4801 tttcggaccg agggtgcggc ggtcgtcagc ctctcaacgg caccactggc tcgggtggcg
     4861 cgcgacggcg acgcagcgga tactttcgaa caggcgatta ccaggctggt gggcctgatc
     4921 gagccgttct accagacgcg aacgctcttc gagttcaagc gcaagttctc gccccgctgg
     4981 gagccggtct acatcgcgtt ccccggcgcg acctgcctgc ccagaattgg ctatgcgatc
     5041 ctgcgcgcct acctgccgac cctgagcatc gaagatatcc gggacctgct agctccagca
     5101 cgtgccggcc gcccgaaacc gcgcgaaggc gtgcccgatc accagtccga caccgaatca
     5161 acaacggcct gatctcgacg acggttagac cgaggttaga ctgatttgaa gtgctcgaac
     5221 ggctgcttgc agtcggggca gtagtagatc gcgcgacaga gggtcgggcc gaaggggttc
     5281 tccatccgcg tgtggcgccc gccgcagaac gggcacggca cgaccgggtg gagcgcgagc
     5341 ggaaagaggt tatcgccgag gctcggggca ggcgtcaccg gcggcgcata gccggccgcg
     5401 cgcaggttct cgcggccccg ctcggtgatc cggtcgctgg tccactgttc ggtataggta
     5461 acgcgaacct cgacctcgtc ggcgatctgc cgcagccgct gctcgacatc ggcacacatg
     5521 atcccgatcg cagggcagcc gatgaaggtc ggcatgatct cgaccgagac acgcccgccg
     5581 tcaatctcta ccgagcgaat gatccccaaa tcaacgaccg agatagccgg tatctccgga
     5641 tcaggcacat cctggagcgc gtcccaaatc tgttgttcgg tgatggcggt cgatgttgtc
     5701 atgatgctca gtcctcgttg ttgctagctc caccacaggc tatttcgcgt aggggcgacg
     5761 gccgcagcct cgccctgct
//
LOCUS       DASUGJ010000233        12231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1006432, whole genome shotgun sequence.
ACCESSION   DASUGJ010000233 DASUGJ010000000
VERSION     DASUGJ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12231
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006432"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFV93_05105"
     CDS             complement(<1..405)
                     /locus_tag="VFV93_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEX5164754.1"
                     /translation="MATKDAQSGSYPTLGVRPRLPSRVTRHASRITIIALIITASLLL
                     AGCKDQNGSIGSPIPTATKPAATQSASPLASGVATEPPTVTATAFATGTATATTSASP
                     TEPVAQGEPPVYGYRIVNSYPHDPAAFTQGLDI"
     gene            554..2833
                     /gene="metE"
                     /locus_tag="VFV93_05110"
     CDS             554..2833
                     /gene="metE"
                     /locus_tag="VFV93_05110"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX5164755.1"
                     /translation="MAQTSNLGYPRIGKQRELKRVIERYWSGKAGEDEVHAVAKEIRR
                     AAWAVQQQAGIDQIPCNDFTYYDHILDTVCLWGMVPERFDHPAGEPVSLATYSAMARG
                     GNHVAALEMTKWFDTNYHYLVPEFDGPAPRRAGNGPVDAYNEAKSQVGKATRPVIVGP
                     LTFVLLGKHHTKSIAEHVNELVPLYRDLLADLAAAGAEWVQIDEPCLVQDRTSDELAL
                     YSSVYTELAGSASRPKIMLQTYFGSLGTNWDTVMSLPVDGIGLDLVRDGGNLSDLQRK
                     GFPQDKALGAGVLNGRAVWKADLNKALGLLESIAETVDKDRIIVGPSASLLFLPHDVR
                     LESSHDASVLANLSFAEQRLDELVILAKGLNEGRDAIAAELKADAERLANLEASVNTT
                     TRERMASLSDADFRRSAPFSERIAAQQQHIDLPPYPTTTIGSFPQSPDVRSMRLKVRS
                     GKITAEEYEAWVHGKMDELVAQQEAVGLDVLVHGEFERSDMVEYFGEHLGGFITTEHG
                     WVQSYGSRCVRPPVIVGDVYRDHPMTVNEVVYAQSKTKKPMKGMLTGPVTILNWSFVR
                     DDMPRSEVCNQIALALRDEVADLEAAGIRIIQIDEPALREGLPLRHAQWADYLDWAVK
                     AFRLSAGGAAPGTQVHTHMCYSEFNDIIDSINAMDADVISIENSRSNEELLRAFTNNK
                     YERMIGPGVYDVHSPAVPATDEIVARLTHAAGVLGSDILWVNPDCGLKTRKDEEVWPS
                     LEHMVAAAKAMRETVAAGK"
     gene            complement(3276..4286)
                     /locus_tag="VFV93_05115"
     CDS             complement(3276..4286)
                     /locus_tag="VFV93_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164756.1"
                     /translation="MSSYPRFRLIMVIFVLGLLALPGTVSAKPKPGAYTITDLGTLGG
                     TDSFAQWINEHGQIVGQGQIVSGEFHAALWENGVPTDLGTLGGPNSFAERINNKGQIS
                     GWSDLADGRFHAVLWEKKGTMIDLGTLGGDMSAATVVNDRGQVVGSADTADGVPHTFL
                     WTRRDGMIDLGTLGGPTSYANGINNKGQVVGNSDTTGGEFHAYLWDNGVMTDLGTLGG
                     PNSLATEIDDQGRVVGFADTADGETHAFLWQDGVMTDLGDFGGTFSLAAATNPRGQVV
                     GGADTAPGGPPHAFIWQNGVMTDIGTLGGDFSVAWGINNKGQIVGQSQGTNGEFHAVL
                     WQ"
     gene            4516..7632
                     /locus_tag="VFV93_05120"
     CDS             4516..7632
                     /locus_tag="VFV93_05120"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX5164757.1"
                     /translation="MLATDTRSLQPAGDYQLRLQLMGHFRVTLGEREIDASVWRLRKA
                     AAIVKLLALDPSRQLHHEQLMDVLWPDVDPAAAANSLRRTLHVARRILIQRDDAASPW
                     LRRHGDVLSLGPPDLVATDVDAFEAAVRRVRNTGDVALYRQAIDLYSGDLLPEDRYEE
                     WAEQRRSSLRASYLALLARLAIIYEQDGQLDEAIEPLQRLIALDPAYEEAHNALMRIY
                     SMTGQRRRALSQYQHLRDALQRELDVEPAIESQQLYQQILSGQSIARPPAPAAPLVAL
                     DSRRHSQPPTNLPIQLTSFIGRTRQVEEITRLLSHEEPVDLAERPAGRPRVVTLTGPG
                     GSGKSRLAIEVAARLRREFAGGVWMVELAPLHDPTLLALHLLAGIPPLHEEPGRLAID
                     TLIDWFDKRRSLLVLDNCEHLIDACARLVERLAHACPELTILATSREALRIPGEALWP
                     VPPLAVPSADQPDSPERLRDLDGPRLFVERAIAANPRFTITDANAPVVAEISRKLDGL
                     PPAIELAATRVRVLTLEEIDSRLDDALRLLTDGSRTAPARHHSLRATLDWSYDLLTPE
                     EQVLLRRLSVFAGGWMLDAAEAICAGDDLAGDSLLNLHAHLVDKSLIEVDETGPQARY
                     RLLEPVRQYAAERLGLAGELDLVRDRHARWCKELGEASFREFGISSEAIRYWATRLEQ
                     EIDNVRSALRWLLRDEDPSLRTPAGVDAALDLALSLHWLWQDGHTVEAQRWFQRLLSE
                     ANPDTTSFRLYVRAIDNVGFHARRSGSFALATTMHERGLALARQHGDRFVESMSLWGL
                     AAIAREQADYDLANDRYRQGLSIIRELGIQNNIAQTLVDWGDALVRQGSIDAADACLG
                     EVLTIVEQHPNLVHMRGQALAELTGVALARGELATARAWADETLAFSRGINRTWAIAN
                     ALAKLGRVAFAQDDEIVATAAWCESLERFHAQRDMRFIPPGLEGLAHIANARNQHDHA
                     TVLIGAASALREKIGTPLPPIDRPAMDQAVAALHAALGDSFDRLYAEGAALSLDEAVA
                     YALRTVED"
     gene            7806..9035
                     /locus_tag="VFV93_05125"
     CDS             7806..9035
                     /locus_tag="VFV93_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5164758.1"
                     /translation="MFRRRWITVVAAVSIASAALALPAAADLDLIAGDWAEVAYTAGE
                     GVNVREAPDPDAAVVAGLPEGFGLTIEDGPVEAWDGSVWYAVWAELEDGGVYGWVSAA
                     YLSGWVDGDADVVEGPVIDSTAPSGVPLYVNTGGEWLNVRSEPSLAAGIVTSIPDGAE
                     VEILAPVIFDDEGIGWTLVGYEGIIGYSVSSYLEGYPAGRERLGDGLLVVSGTDGDGL
                     SLRAWPAADGELLAVLDEGSQAWLLDGPVTGDDGESWYQIEVDGLVGWSHGTYLGATG
                     GEMELAEASTTGAAIVEAALGYLGVPYVWGGTTPEGFDCSGFTWFIVSNVVDTSFPRP
                     NEDQVVSGVEIGQGDLVPGDLIFFQNTYQWGLSHVGIYLGDGQFVSASGEHDGVGISS
                     LSDPYWATRYLTARRVT"
     gene            complement(9053..9442)
                     /locus_tag="VFV93_05130"
     CDS             complement(9053..9442)
                     /locus_tag="VFV93_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX5164759.1"
                     /translation="MSKVPSHRDLIVWQKAMDLVVLVYELATMLPPGERYGMKSQMTR
                     AAVSVPANIAEGKGRGTQREYVHFLRIARGSLMELDTYIELVDRLGYADSIVTAPIRA
                     LIVQIGKMLSAVRTRLSIPLPSDPPNT"
     gene            complement(9494..10999)
                     /locus_tag="VFV93_05135"
     CDS             complement(9494..10999)
                     /locus_tag="VFV93_05135"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX5164760.1"
                     /translation="MRVAIGGIAHESSTFATVPTTLDDFLRRGYQEDAAMIEELSGTK
                     SAIGGFIDAATTMGFEVVPTIMASAQPGGTVTAEATETLVGRLVERIRAAHAEGPLDG
                     VLLALHGAMVSELDDDGESYILRAVREAIPPETPILVTLDLHGNITQEMVDLTTIPIA
                     YDEYPHTDPYERAYEAGLLLAKIVRGGAKPTSAVVKIPMLAGGQRQYTHAEPMLSVKH
                     LAHDIESVRGVLNVSYLPCFPWADIEPTMFTVIVTTDNNPQQAEAAAKRLARYIWQHR
                     AEFIVRPTPVDDAVRRAMAVPEGPIVLADIGDNPGGGTPADGTVLLEALLRLGATRTV
                     ITPMADPAVVQQAIAAGEGATFETQIGGKVDTYHGAPLNVTARVIRLTDGSWVNKGLM
                     ATGVHQSVGPTAVLEIAGQHGGSVIAITTTYRQQPTDLEMLRSQGIEPTAQQILVVKS
                     SVHYRSAFTPIAKEIIEVDTPGLSSPHIDTLVFHKLQRPIYPFDVDMDWEP"
     gene            11151..11984
                     /locus_tag="VFV93_05140"
     CDS             11151..11984
                     /locus_tag="VFV93_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164761.1"
                     /translation="MSQRFDPRQHLRELHDGTAYLDVKWRLHWLRSEHPEAVIETQLV
                     SLDGDTAVCKATVRIPDGGSATGHASAARGAGASADHIEHAETRAIGRALAALGYGAQ
                     FTEDDALRGRPMAADRPVERPVSLVTPPVTTQPQPPTRMRPVRDDDDDEPEPTPPPTP
                     RPEPREPVDVRDSRGPRESRPVREVREMRDEDESDKPEPTPLKRGSDLRQRDQPTGDS
                     APAREDVSWTRFWEWAKRHGYRDAAHLRDLLGIDVNALTPGEVRAHIKRYELDHPPGE
                     E"
     gene            complement(12027..>12231)
                     /locus_tag="VFV93_05145"
     CDS             complement(12027..>12231)
                     /locus_tag="VFV93_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164762.1"
                     /translation="RATGDHARLGEGHAANDGSFDIPLDPAPWPLESITGDAYKTLNT
                     TLECRAGAGPWTQPLRPPRVGIN"
BASE COUNT         2046 a         3935 c         4130 g         2120 t
ORIGIN      
        1 gatgtccagc ccctgggtga acgcggccgg gtcatgtgga tagctgttca cgatccggta
       61 gccatagact ggcggttcgc cctgggctac tggttcggtc gggcttgcgg atgtcgtcgc
      121 cgttgccgtc ccggtcgcga aggccgtcgc ggtgacggtc ggtggctcgg tcgcgacgcc
      181 cgatgcgagc ggcgatgcgc tctgcgtcgc cgccggtttg gttgcagtgg gaattggcga
      241 cccaatgctg ccgttttgat ccttgcagcc ggcgaggagc agcgaagcgg tgatgatgag
      301 ggcgatgatc gtgatgcgtg atgcgtgacg cgtgacgcgc gaaggaaggc ggggacggac
      361 gccgagcgtg ggataagaac cggattgggc gtccttcgtt gccacgcatc tctcctgacg
      421 caaatcctac gtcacgaatc tcgcgtcacg caccacgcgt cacgcgtcac gattactccg
      481 tcccgagtcc cgccactatg ccatacttca tcgtcgcaat ttcgtccact ggtggaggga
      541 taagagggat tctgtggcac agacgagcaa cctgggatac ccgcgcatcg ggaagcagcg
      601 cgagctgaag cgggtcatcg agcgctactg gtccggcaag gcaggcgagg acgaggtcca
      661 cgccgtcgcg aaggagattc gccgggcggc ctgggccgtt cagcagcagg ccgggatcga
      721 tcagattccc tgcaatgact tcacgtacta cgaccacatc ctcgataccg tctgcctctg
      781 ggggatggtt ccggagcgct tcgaccaccc agccggcgag ccggtctcgc tggcgaccta
      841 ctcggcgatg gcacgtggcg ggaaccacgt cgccgcgctt gagatgacca agtggttcga
      901 caccaattac cactacctcg tgccggagtt cgacggcccg gccccgcggc gcgccggcaa
      961 cggcccggtc gatgcctaca atgaggcgaa gtcccaggtc ggcaaggcaa cccggccagt
     1021 catcgttggc ccgctgacgt tcgtcctgct cggcaagcac cacacgaagt cgatcgccga
     1081 gcacgtcaac gagctggtgc cgctctaccg cgacctgctg gccgacctgg ccgccgccgg
     1141 cgccgagtgg gttcagatcg acgagccgtg cctcgtccag gaccgcacct ccgacgagct
     1201 ggcgctctac agcagtgtct ataccgagct tgccggatcg gcatcgcggc cgaagatcat
     1261 gttgcagacc tacttcggct cgctcgggac gaactgggac acggtcatgt cgctgccggt
     1321 cgatggcatc ggcctcgacc tcgttcgcga cggcggcaac ctgagcgacc tgcagcgcaa
     1381 gggcttccca caggacaagg cgctcggggc tggtgtcctg aacggtcgcg ccgtctggaa
     1441 ggccgacctc aacaaggcgc tcggcctgct ggaatcgatc gccgagacgg tcgataagga
     1501 ccggatcatc gttggcccgt ccgcctcgct gctcttcctg ccgcacgatg tccggctgga
     1561 gtcgagccac gacgcgtcgg tcctggcgaa cctctccttt gccgagcagc ggctggacga
     1621 gctggtgatc ctggcgaagg gcctcaacga gggccgcgac gccatcgccg ccgagctgaa
     1681 ggcggacgcc gagcggctgg cgaacctcga agcgtccgtc aacacgacga cccgcgagcg
     1741 gatggcgtcg ctgagcgatg ccgacttccg ccgctcggcg ccgttcagcg agcggatcgc
     1801 cgcccagcag cagcacatcg acctgccgcc ctacccaacg acgacgatcg gctccttccc
     1861 gcagtcgcca gacgtgcgct cgatgcggct caaggtccgc tccggcaaga tcacggcgga
     1921 ggagtacgag gcctgggtcc acggcaagat ggacgaactg gtcgcccagc aggaggcggt
     1981 cgggctggac gtgctggtgc atggcgagtt cgagcgttcc gacatggtcg agtacttcgg
     2041 cgagcacctg ggcggcttca tcacgaccga gcacggctgg gtccagtcat acggctcgcg
     2101 ctgcgtccgc ccgccggtga ttgtcggcga cgtctaccgc gatcatccga tgacggtcaa
     2161 cgaggtcgtc tacgcgcagt cgaagacgaa gaagccgatg aaggggatgc tgaccggtcc
     2221 ggtcacgatc ctcaactggt cgttcgtccg tgacgacatg ccccgctcgg aggtctgcaa
     2281 ccagatcgcg ctggcgctgc gggacgaggt cgccgatctc gaagcggccg ggatccgcat
     2341 catccagatc gatgagccgg cgctccgcga gggcttgccg ctacggcacg cgcagtgggc
     2401 cgactacctc gactgggccg tcaaggcgtt ccggctctcg gccggtggcg ctgcgcccgg
     2461 cacgcaggtc cacacgcaca tgtgctactc ggagttcaac gacatcatcg acagcatcaa
     2521 cgcgatggat gccgacgtga tctcgatcga gaactcacgc tcgaacgagg agctgctgcg
     2581 tgccttcacc aacaacaagt acgagcggat gatcggcccc ggcgtctacg acgtccactc
     2641 cccggccgtt ccggcgacag atgagatcgt cgcccggctg acccacgcgg ccggcgtcct
     2701 cggcagcgac atcctctggg tcaaccccga ctgcggcctc aagacccgca aggacgagga
     2761 agtctggccc agcctggagc acatggtcgc cgccgcgaag gcgatgcgcg agacggtcgc
     2821 ggccggcaag tagtctcgtc catcacgagc gcccgaccac tggaggtcgg gcgcttgtcg
     2881 tgcatgcgag ccgacgccag caacactcag ccgaaagcag accgttccgg tcgctgggtc
     2941 agccgacacg cgcgcgtacc caaacccgtc gcgatctccg ggcggcaact cggagtcacc
     3001 ttggagttgt gcatgcaacg agatcgccgt cgtcggcgtg aggtccccag cctggacggt
     3061 cgcgattgag atgaacatca ggatcgcgac gcttgccgta gtcacgatag ccctgtaggg
     3121 gatcatacgg ctctccgttc aaaatctgcg tggtcgggtg aagaatgtcc cgctccgccg
     3181 cctccgctag agacgcttgt ggtgttgagc agggacggag ccgcgggcac tctggatgat
     3241 ggagtgcctg cggctacgac gttacgcaac tagtgttatt gccagaggac ggcgtggaac
     3301 tcgccgttcg tcccttgcga ttgaccgacg atctggccct tgttgttgat cccccaggcg
     3361 acgctgaagt cgccgcccag cgttccgatg tcggtcatga cgccgttctg ccagatgaac
     3421 gcgtgcggtg ggccgcctgg tgcagtgtcg gcgccaccga cgacctgccc gcgtgggttc
     3481 gtggccgcgg cgaggctgaa cgttccgccg aagtccccaa ggtcagtcat gacgccgtct
     3541 tgccagagga aggcgtgcgt ctcgccgtcg gcggtgtcgg caaagccgac gacccggccc
     3601 tgatcgtcga tctcggtcgc gaggctgttc ggtccaccca gtgtgccaag atctgtcatg
     3661 acgccgttgt cccagaggta ggcgtggaac tcgccgcccg tcgtgtcgct atttcctacc
     3721 acctggccct tgttgttgat tccgttcgcg tagctcgttg ggccgccgag tgtcccgagg
     3781 tcgatcatgc catcacgacg tgtccagagg aacgtatgtg gcacgccgtc ggcggtatcg
     3841 gcggagccga cgacctgccc ccggtcgttg acaaccgtcg cggcgctcat gtcgcctccc
     3901 agcgtcccga ggtcgatcat cgtccccttc ttctcccaga ggacagcgtg gaatctgccg
     3961 tccgccaggt ccgaccagcc cgagatctgg cctttgttgt tgatccgctc ggcgaagctg
     4021 tttggcccgc cgagagttcc gaggtcagtc ggtacgccgt tttcccagag cgccgcatgg
     4081 aactcgccac tgacgatctg gccctgtcca acgatctggc catgctcgtt aatccactgg
     4141 gcgaagctat cggtgccacc gagtgtcccc agatcggtga tggtataggc cccaggcttc
     4201 ggtttggccg acactgtgcc tggcagcgcc agcaatccca acacgaaaat gaccatgatg
     4261 aggcggaatc gaggatagga cgacatgtgg cctcccttat gggtcgcgtt cgcccggccg
     4321 agcggccgag cggttggcga gtggaatgtt gataaaccgt agcgggatga tgaccctcca
     4381 gcattacctg cgcgttactt gacatgaacc tgaattgcca gatactgaca gcatcgagcg
     4441 gaagtgaatc ggattgcgtg gccgcgactg tcgagtttgc acgccactgc ttccacgtcc
     4501 gtgtcggagg gccaggtgct ggcgacggat acgcgctcgc tccaaccggc aggcgattac
     4561 cagcttcgac tgcaactgat ggggcacttc cgcgtcacgc ttggagagcg ggagatcgac
     4621 gcctcggtct ggcggctgcg caaggcggcg gcgatcgtca agctgctggc gctcgacccg
     4681 tcgcggcagt tgcaccacga gcagttgatg gacgtcctct ggccggacgt cgatcccgcc
     4741 gccgccgcca acagcctgcg ccggacgctg catgttgccc ggaggattct gattcaacgt
     4801 gacgacgctg cgtcaccgtg gctgcggcgc cacggcgatg tcctctcgct gggaccgccc
     4861 gatctcgtcg cgactgatgt ggatgcgttc gaggcggcag tccggcgcgt ccgtaacacg
     4921 ggcgacgttg ccctctaccg gcaggcgatc gatctttatt ccggcgattt gctgcccgaa
     4981 gatcgctacg aggagtgggc ggagcagcgg cgttcgagct tgcgtgccag ctacctggcg
     5041 ctgctggcgc ggctggcaat catctacgag caggacgggc agctggatga ggcgattgag
     5101 ccgctgcaac ggctgatcgc gcttgaccct gcctacgagg aagcccacaa cgcgctgatg
     5161 cggatctact cgatgaccgg ccagcgacgg cgcgcgctca gtcagtatca gcatcttcgc
     5221 gatgcgctcc agcgcgaact ggatgtggaa ccggcgatcg agagccagca gctgtaccag
     5281 cagatcctga gtggacaatc catcgctcgt ccgccggcac ctgccgcgcc gttggtggca
     5341 ctcgacagcc gtcgccattc gcaaccgccg accaacctgc caatacagtt gacgagcttc
     5401 atcggccgca cacggcaggt cgaagagatc acacggctgt tgagtcacga ggagccggtc
     5461 gacctggcgg aacggccggc tggccggccg cgtgtcgtga ccttgaccgg tcctggcggc
     5521 agtggcaagt cgcggctggc gatcgaggtc gccgcacggc tgcgccggga gttcgcgggc
     5581 ggcgtctgga tggtggaact cgcgccgctg cacgacccga cgctgctggc gctgcacctg
     5641 ctggccggca tcccgccgct gcacgaggag cctggccgtc tggcgatcga tacgctgatc
     5701 gactggttcg acaagcgacg atcgttgctg gtgctggaca actgcgagca tctgatcgac
     5761 gcatgcgcgc gactcgtcga gcgattggca cacgcctgtc cggagttgac gatcctggcg
     5821 actagtcgcg aggcgctgcg gatccccggc gaggcgctct ggccggtgcc gccgctggca
     5881 gttcccagcg cagatcaacc ggactcgccg gaacgtttgc gggacctgga tggccctcga
     5941 ctgttcgtcg aacgggccat cgcggcgaac ccgcgtttca cgatcaccga tgcgaacgcg
     6001 ccggttgtgg ccgagatcag tcgcaagctg gacggcctgc ctccggcgat cgagctggcc
     6061 gcgacgcgcg tccgtgtcct cacgctggag gagatcgata gtcgcctcga tgatgcgttg
     6121 cggctgttga ccgatggttc acggacagcg ccggcgcgcc accacagcct gcgcgcgacg
     6181 ctcgactgga gctacgacct gctgaccccg gaagagcagg tgttgctgcg gcggctctct
     6241 gtcttcgccg gtggctggat gcttgacgcg gccgaagcca tctgcgccgg cgacgatctg
     6301 gccggggaca gtctgctcaa cctgcacgcg cacctggtcg acaagtcact gatcgaggtc
     6361 gacgagaccg gcccacaggc ccgctaccgg ttgctggagc cggtccggca gtatgcggcc
     6421 gagcgacttg gcctggcagg tgaactggat ctcgttcgcg accggcacgc caggtggtgc
     6481 aaggaactgg gcgaggcgtc gtttcgggag ttcggtatct cgtccgaggc gatccgctac
     6541 tgggccacac gactagagca ggagatcgac aacgtgcgat cggcgctgcg ctggttgctg
     6601 cgcgacgaag atccgtcgct gaggacgccg gctggagtcg atgcggcgct cgatctggcg
     6661 ctgagcctcc attggctctg gcaggacgga cacacagttg aagcgcagcg ctggttccag
     6721 cggctgttgt ccgaggcgaa tccggacacg acctcgtttc gcctctacgt ccgggcgatc
     6781 gacaacgtcg gctttcacgc gcgccgatcc ggcagcttcg cactggcgac gacgatgcac
     6841 gagcgcgggc tcgcgctggc ccgccagcat ggcgaccggt tcgttgagtc aatgtcgctc
     6901 tggggactgg cagcgatcgc tcgggagcag gccgactacg acctggcgaa cgaccgctac
     6961 cggcagggtc tgtcgatcat ccgggagctg ggcatccaga acaacatcgc ccagacgctt
     7021 gtggactggg gagacgcgct cgtgcgccag ggatccatcg acgcggccga cgcatgcctc
     7081 ggcgaggtgc tgacgatcgt cgagcagcat cccaacctcg tccacatgcg cggtcaggct
     7141 ctggcggaac tgacaggcgt ggcgctcgcg cgcggagagc tggcgaccgc gcgcgcctgg
     7201 gccgatgaga cgctggcgtt cagtcgaggc atcaaccgta cctgggcgat cgcaaacgct
     7261 ctggccaagc ttggcagggt cgccttcgcc caggacgatg aaatcgtcgc aaccgcagcc
     7321 tggtgcgaga gcttggagcg attccatgct cagcgcgata tgcgcttcat cccgcccggc
     7381 ctcgaagggc tggcccacat cgccaatgct cggaatcaac atgaccatgc gaccgttctg
     7441 atcggcgcgg caagcgcgct ccgcgagaag attgggacgc cactaccacc catcgatcgc
     7501 ccagcgatgg atcaagcggt tgccgcgtta catgccgcgc tgggcgacag cttcgaccgg
     7561 ctctacgccg aaggggcggc gctctcgctt gacgaagcgg ttgcgtacgc cctccgaacc
     7621 gtcgaagact aacactagcc ccacaattct cagctcccgt tcaggcaggg cttgccgggg
     7681 cggggtagac tgtacgtaca gcctgtgtct ggtaggcaca gccggactac gtggtgcggt
     7741 gctgccgtta ctgcgtggcg ggacgcggcg tggggccgtg gacgaaaggg gagctgctgg
     7801 tggggttgtt tcgacgacga tggataacgg tcgtcgcggc tgtgtcgatc gcgagtgctg
     7861 cgctcgcgtt gccggcggct gccgatctgg acctgatcgc gggtgactgg gctgaggtcg
     7921 cctatacagc gggtgaaggt gtcaacgtcc gtgaggcgcc ggacccggac gcggccgtcg
     7981 tcgccggctt gccggagggg ttcgggctga cgatcgagga cggcccggtc gaggcctggg
     8041 acggctcggt ctggtacgcc gtctgggccg agctggaaga tggtggcgtc tacggctggg
     8101 tcagcgcggc ctacctgagt ggctgggtcg atggtgacgc ggacgtggtc gaaggcccgg
     8161 tcatcgacag cacggcgccc agcggtgtcc cgctctacgt caacaccggc ggtgagtggc
     8221 tcaacgtccg cagcgagccg tcgctggcgg ccgggatcgt cacctcgata ccagatggcg
     8281 ccgaggtcga aattctggcg ccagtcatct tcgatgacga gggtatcggc tggacgctgg
     8341 tcggctacga gggcatcatc ggctacagtg tctcgtccta cctggagggc tatccggccg
     8401 gccgtgaacg tctcggcgat ggcctgctgg tcgtcagcgg gacggatggc gacgggctca
     8461 gcctgcgcgc ctggccggcg gccgatggcg agctgctggc ggtcctcgac gagggcagcc
     8521 aggcctggct gctggacggt ccggtgacgg gtgacgacgg cgagagctgg tatcagatcg
     8581 aggtcgatgg gctggttggc tggtcgcatg ggacttacct cggcgcgacc ggcggcgaaa
     8641 tggagctggc cgaggcatcg acgaccggcg cagccatcgt cgaggcggcg ctcggctacc
     8701 tcggcgttcc gtacgtctgg ggcgggacga cgccggaggg cttcgactgc tccggcttca
     8761 cctggttcat cgtcagcaac gtcgtcgaca cgagcttccc gcgcccgaac gaggaccagg
     8821 tcgtctccgg cgtcgagatc ggccagggcg atctggtgcc cggcgacctg atcttcttcc
     8881 agaacaccta ccagtggggc ctctcgcacg tcggcatcta tctcggcgac ggccagttcg
     8941 tcagcgccag cggcgagcac gacggcgtcg gcatcagcag cctctccgac ccgtactggg
     9001 cgactcgcta tctgacggcg cggcgcgtga cgtaggttct aggtgatagg tatcaggtgt
     9061 tagggggatc tgacgggagc ggtatcgaca agcgggttcg cacagcagaa agcatcttgc
     9121 cgatctggac gatgagagcc ctgatcggcg cggtcacgat ggagtcggca tagccgaggc
     9181 gatcgacgag ttcgatgtac gtgtcaagct ccatcagtga tccgcgcgca atccgcagga
     9241 aatggacgta ctcacgctgc gtcccgcgcc ctttcccttc ggcgatgttg gcggggacgg
     9301 atacggctgc acgggtcatc tgagatttca ttccgtatcg ttcccctggc ggcagcatcg
     9361 ttgcaagctc gtagaccagt acaacgaggt ccatagcttt ctgccacacg ataagatcac
     9421 gatgcgacgg caccttgctc acgtctcttc ctcaacccct cgaacctaat acctatcacc
     9481 taccacctat tacctacggc tcccagtcca tatccacatc aaacggatag atcgggcgct
     9541 ggagcttgtg gaacacgagg gtgtcgatgt gggggctgga gaggccgggg gtgtcgactt
     9601 cgatgatctc cttggcgatc ggggtgaagg cgctgcggta gtggacggat gacttgacga
     9661 cgaggatctg ctgcgcggtc ggctcgatcc cctgggagcg cagcatctcc aggtcagttg
     9721 gttgctgtcg gtaggtggtg gtgatcgcga tcacgctgcc gccatgctgg ccggcaatct
     9781 cgaggacggc ggttgggccg acgctctggt gaacgccagt cgccatcagc cccttattga
     9841 cccagctgcc gtctgtcagg cggatgacac gggcggtgac gttcagcggt gcgccgtggt
     9901 acgtatcaac cttgccacca atctgtgtct cgaaggttgc cccttcgccg gcggcgatcg
     9961 cctgctggac gacggcggga tcggccatcg gcgtgatgac ggtcctcgtc gcgccgaggc
    10021 gcagcagcgc ttcgagcagg acggtaccgt cggccggtgt gccgccgccc ggattgtcgc
    10081 cgatgtcggc cagcacgatc gggccctcgg gcaccgccat tgcccgccgg acggcgtcat
    10141 ccaccggcgt cgggcggacg atgaactcgg cacggtgctg ccagatgtag cgtgcgagcc
    10201 gtttcgcggc cgcttctgcc tgctgggggt tgttgtcggt tgtgacgatg acggtgaaca
    10261 tcgtcggctc gatatccgcc cacggaaagc acggcaggta cgagacgttc aggacgcccc
    10321 gcacgctctc gatatcgtgg gcgagatgct tgacgctcag catcggttcg gcgtgcgtat
    10381 attgtcgctg cccacccgcc agcatcggga tcttgacgac ggcgctggtc ggcttcgcgc
    10441 cgccccgcac gatcttcgcc agcaacagac cggcctcata tgcgcgctcg tacggatcgg
    10501 tatgcggata ctcgtcatac gcgatcggga tcgtcgtgag gtcgaccatc tcctgggtga
    10561 tgttgccgtg caggtcgagc gtgacgagga taggcgtctc cggtgggatt gcctcgcgga
    10621 cagcgcgcag gatatagctc tcgccgtcat cgtcaagttc gctgaccatc gcgccgtgga
    10681 gtgccagcag cacgccatca agcgggcctt cagcatgggc ggcgcggatg cgctcgacca
    10741 gccgaccgac gagcgtctcg gtcgcctcgg ccgtgacggt gccacccggc tgggcggagg
    10801 ccatgatcgt cgggacgacc tcgaagccca tcgtcgttgc cgcgtcgatg aagccgccga
    10861 tggcagactt ggtgccgctc aactcctcga tcattgcggc gtcttcctga tagccgcgtc
    10921 gaagaaagtc gtccagcgtc gtgggtaccg tcgcgaaggt gctgctctca tgggcgatcc
    10981 cgccgatggc gacacgcatg tttcccgtcc tctcatcatg cgaatgacct gacgggcggc
    11041 agtctaacgc gattaacacg atggcacgag cggcacgctg gctataatga cggtgtcgaa
    11101 cgatgccgcg tcctggccgg cgcgcgatac ggatggagcc tgggcaagcg atgagccagc
    11161 gattcgaccc acgccagcac ctgcgcgaat tgcatgacgg tactgcctac ctcgatgtga
    11221 agtggcggct ccactggctg cgcagcgagc atcccgaagc ggtcatcgaa acccagctcg
    11281 tctcactcga tggcgacacg gccgtctgca aggcgacggt ccggatacca gatggcggct
    11341 cggccaccgg ccacgccagc gccgcgcgtg gggccggagc gagcgccgac cacatcgagc
    11401 atgccgagac ccgcgcgatc ggccgggcac tggcggcgct cggctacggc gcacagttca
    11461 ctgaagatga cgccctccgg ggccgcccga tggcagccga ccggccggtc gagcgccccg
    11521 tctcgctggt gacgccgccg gtgacgaccc aaccccagcc gccgacccga atgcgccccg
    11581 tccgggacga cgatgacgac gagccggaac caacgccgcc cccaacgcca cgtccggagc
    11641 ctcgtgagcc ggtcgatgtc cgtgattcgc gcggcccgcg tgagtcacgt cccgtccgcg
    11701 aagtacgtga gatgcgcgac gaagacgaat ccgacaagcc cgaaccaacg ccgctcaagc
    11761 gcggctccga cctgcgccag cgcgaccagc ccaccggaga cagcgctccg gcccgcgagg
    11821 acgtctcctg gacgcgcttc tgggaatggg ccaagcgcca cggctaccgc gacgccgccc
    11881 acctgcgcga cctgctcggc atcgacgtca acgccctcac ccccggcgaa gtccgcgccc
    11941 acatcaagcg ctacgaactg gaccacccgc cgggcgaaga gtgacgggtg ctgcgtgctg
    12001 aatactcagc acgcagcact gtctactcag ttaatcccaa cccgtggcgg gcggaggggt
    12061 tgggtccagg ggccggcgcc ggcgcggcat tcgagggtgg tgttgagcgt cttgtaggcg
    12121 tcgccagtga ttgattccag cggccagggg gcggggtcga gtgggatgtc gaaggagccg
    12181 tcgttggcgg cgtgaccctc gccgagcctg gcgtgatcgc cggtcgcgcg g
//
LOCUS       DASUGJ010000234         5934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1839252, whole genome shotgun sequence.
ACCESSION   DASUGJ010000234 DASUGJ010000000
VERSION     DASUGJ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839252"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..223
                     /locus_tag="VFV93_05150"
     CDS             26..223
                     /locus_tag="VFV93_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164763.1"
                     /translation="MDWGSALLWGFVGTIFLTGIMASSQGAHLTRMSLPFMLGTIFTR
                     DRDRARLVGCWSTSSTAGSSR"
     gene            184..498
                     /locus_tag="VFV93_05155"
     CDS             184..498
                     /locus_tag="VFV93_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164764.1"
                     /translation="MLVHFVNGWIFALIYVAAFEAWGRAEWWIGAGIGLVQAAFVLTA
                     GMSFLPGMHPRMAGETRGPTPTRQLEPPGFMALNYGVRTPVVIVIAHLVYGAILGAFY
                     AL"
     gene            complement(486..1487)
                     /locus_tag="VFV93_05160"
     CDS             complement(486..1487)
                     /locus_tag="VFV93_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5164765.1"
                     /translation="MEWVGDAPHPWVVERRDRVTFGIQSMARPDDPRPSERVLAAGRL
                     ADTLGLDAVYVSDHPARTTECWTHLAALAVSTERVRLGSVVNCVLYRHPIMTARLATD
                     LDHLSGGRVMLGLGIGWDVPEFGELGIPFPPTPERQGALVEALEVIAGVWGDAPFSYS
                     GTHYQVTNARITPGPTEQPHPPLMIAGAGERVSLRQVARYADACNFGPSPQIGGVLTP
                     DDVRRKYDVLKRHCEEIGRPYENILRTHFTPWLMIAETEAKAQAKLARYYPNGLTEMQ
                     QYSRIYGSPEQVAAYYQSMAEAGVQHFVCQSQDAADTETIQLLAEEVSPRVRSVQSA"
     gene            1933..3729
                     /locus_tag="VFV93_05165"
     CDS             1933..3729
                     /locus_tag="VFV93_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5164766.1"
                     /translation="MNERPYVSAPIDASAGSGLLGRDRFQHAFFAAVIGMAIVTTDGN
                     FLQVNPSLCEMLGYSEDELLGESHQSITHPADVSADEEQIQRLLAGESDSYQIEKRYL
                     HRDGHEIWAELSVSLVRNENGRPRYCIAQMVNITERKLVEQQLAHQAFHDGLTGLPNR
                     ALFMDRLEHALASRRRADEMIAVIFLDLDNFKVINDSLGHRAGDQLLLTMAARLQTCV
                     REGDTVARLGGDEFTVLLEQITDLSDVIRVAQRIAEQSLTLYNIGDREVSVTASIGIA
                     LPGDDNETADDLLRNADIAMYEAKRRGKAQYAVFSAPMNVRARERLELEIELQRAIER
                     GEFVLHYQPVVDLRTGRIAAVEALIRWRHPLRGLLPPADFIAIADETGSILDIGGWVL
                     RRACRKLQQWQEEFPAPAGQRPLMMSVNLSTRQFLQASLVETVREVLDETGIEPRTLK
                     LEVTERVIMEDAPSAIATLRDLRELGITLAIDDFGIGYSSLTDLKQFAVDLLKIDRTF
                     VDGISQDMDDTSIVTAIIAIGKSLGIRTVAEGVETPAQLAFLTTRGCDSGQGHLFSPP
                     LPPEEIATLLRNQTNYQIPRPITSRPLTFYNS"
     gene            complement(3734..4177)
                     /locus_tag="VFV93_05170"
     CDS             complement(3734..4177)
                     /locus_tag="VFV93_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5164767.1"
                     /translation="MIRRFPQYPRLVFVDTSALFAMVDRDDDFHSSVVAATEEIVAAG
                     GQLVVTNFVFAELHALLLSRINRHVARTALTRLRDSHDVSLVRVSVDDEQRAWEIIDR
                     FTDKDFSFADASSFAVMECLGITHALSLDAHFVQYGWVMLPVDRE"
     gene            complement(4174..4452)
                     /locus_tag="VFV93_05175"
     CDS             complement(4174..4452)
                     /locus_tag="VFV93_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164768.1"
                     /translation="MAAERTMIHAGVDSELAEALRTASEARKPIFINTGDVVYEVHVH
                     AIEPIERFNRDKPHSILDIAGLGASGSSGSIADHKDEYIADAYDVKRR"
     gene            complement(4610..4876)
                     /locus_tag="VFV93_05180"
     CDS             complement(4610..4876)
                     /locus_tag="VFV93_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164769.1"
                     /translation="MAVYINSESELAQTLRKAAASGESIIIETDDAQYEIDIRVRWDA
                     ENADASDEPDAILELIGIVESREPSNIAEFKDQYIADAIDPRLR"
     gene            complement(4957..>5934)
                     /locus_tag="VFV93_05185"
     CDS             complement(4957..>5934)
                     /locus_tag="VFV93_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164770.1"
                     /translation="LSDRPVYFVSSNLHSIANILSGTARRRRDELIAFIEDSQHPDLL
                     PELHKLQSGDTRSSWDNFLYFAARLYYTANGPDRPSRNREEEERGITTIEQDSALDVG
                     IQIIELDRLDAADLDPRILKVCGGPPDTDAIIVNINYPLGLAAYHILCQVSAEVAQLR
                     GVYVLGKAATLNARIGDVMISDVVYDEHSDNTYWFANSFSSVDLEPFLVYGSTLDHQR
                     AVTVKGTYLQNRGYLDFYYRENYTVVEMEAGPFLSALYEDSYLTRHPQSESVNLSQVP
                     IDIGIIHYASDTPYSHAHTLGARGMSYRGMDSTYAATLAIVRRVFESCR"
BASE COUNT         1074 a         1874 c         1920 g         1066 t
ORIGIN      
        1 gcaaccacgg taggaggtca tccacgtgga ctgggggagc gccctcttgt ggggcttcgt
       61 cgggacaatc ttcctgaccg gcatcatggc cagtagtcag ggcgcgcacc tgacgcggat
      121 gagcctgccg ttcatgctcg ggacgatctt cactcgcgac cgcgaccgtg ccaggctggt
      181 gggatgctgg tccacttcgt caacggctgg atcttcgcgc tgatctacgt cgctgccttc
      241 gaggcgtggg gacgggccga atggtggatc ggcgccggaa tcgggctggt ccaggccgcg
      301 ttcgtcctga cggccgggat gtcgttcctg cccggcatgc acccccggat ggccggcgag
      361 acgcgcggcc caacgccgac gcgccagctc gaaccgcccg ggttcatggc cctcaactac
      421 ggcgtgcgca ctcctgtcgt gatcgtcatc gctcacctgg tctacggcgc catcctcggc
      481 gcgttctacg cgctctgaac tgatctcacg cggggggaga cctcctcggc gaggagctgg
      541 atcgtctccg tgtcggcagc gtcctgggac tggcagacga agtgctggac gccggcctcg
      601 gccatcgact ggtagtaggc ggccacctgt tccggcgagc cgtagatgcg gctgtattgc
      661 tgcatctcag tcagcccgtt ggggtagtag cgcgccagct tggcctgcgc cttcgcttcg
      721 gtttcggcga tcatcagcca aggggtgaag tgcgtccgca ggatgttctc atacggccgg
      781 ccgatctcct cgcaatgccg cttgaggacg tcgtatttgc gccggacatc gtcgggtgtc
      841 agcacgccgc cgatctgtgg tgaagggccg aagttgcagg cgtcggcata gcgggcgacc
      901 tgccgcaacg agacgcgctc gccggctccg gcgatcatca gcggtgggtg cggctgttcg
      961 gtcgggccgg gtgtgatgcg cgcgttcgtc acctggtagt gcgtgccgct gtagctgaac
     1021 ggcgcgtcgc cccagacacc ggcgatgacc tccaatgcct cgaccagcgc gccctggcgc
     1081 tccggcgtcg gtggaaacgg gatgcccaac tcgccgaact ctggcacatc ccagccgatc
     1141 ccgagcccga gcatcacccg gccgccgctg agatggtcca ggtcggtcgc cagccgggcc
     1201 gtcatgatcg ggtggcgata gaggacgcag ttgacgaccg atcccagccg aacccgctcg
     1261 gtcgagacgg ccagcgccgc caggtgagtc cagcactcag tcgtccgggc cgggtgatcg
     1321 ctgacgtaga cggcgtcgag ccccaacgtg tcggccagcc ggccagcggc gaggacccgc
     1381 tcgctcggcc ggggatcgtc ggggcgggcc atcgactgga tgccgaaggt cactctgtca
     1441 cggcgctcga caacccacgg atgcggtgcg tcgcctaccc attccatctg gacggtcctt
     1501 tcacatctgg cggcaccggt cggtgggaat cgtcgatccg ctccggtcag aggcgatctt
     1561 gcgctggcgc gcccggatcg gcaagtacca gcctccatcc gatagatggg accacaggag
     1621 tgattagccg aaagataaca atcgccgttc cgctcggcgc gacgtatcat ggatggcgtc
     1681 gcgcaattcc acccgtcgtc caggcagatg ctttgcaggc aattgccggc gatcagacgg
     1741 tcatgcgggc gcgggaaccc atggcggcag gacggcaacg tctttgcccc tcccaggcag
     1801 tcagtgtcag agagagcgtt ggatggacgc gagcgttccg gcccaccctc aatcccgccg
     1861 tgccactcct gcaacgtcct tgcctgttca tggccggcca acgcaggtgc gatcacgcat
     1921 cacggccgtc ccgtgaacga gcgcccgtac gtctccgcac cgatcgacgc aagcgccgga
     1981 agtggcctgc tggggcgcga ccggttccag catgcgttct tcgcggccgt gatcgggatg
     2041 gcgatcgtca caaccgacgg caacttcctc caggtcaacc catccctctg cgagatgctc
     2101 ggctactccg aggacgagtt gctcggcgag tcgcatcagt cgatcaccca tccggccgat
     2161 gtcagcgcgg acgaggagca gatccagcgc ttgctggccg gtgagagcga tagctatcag
     2221 atcgagaagc gctacctgca ccgcgatggc cacgagatct gggccgagct ctccgtctcg
     2281 ctggtccgca acgagaatgg ccggccgcgc tactgcatcg cccagatggt caacatcacc
     2341 gagcgcaagc tggtcgagca gcagctcgcc caccaggcat tccacgacgg gctgaccggc
     2401 ctgcccaacc gggcactgtt catggaccgg ctggaacacg cactcgccag ccgccgccgc
     2461 gcggacgaga tgatcgccgt catcttcctc gacctggaca acttcaaggt catcaacgac
     2521 agcctcggcc accgcgccgg cgaccagcta ctgctgacga tggctgcacg gcttcagacc
     2581 tgcgtccgtg agggcgacac cgtcgcccgg ctgggcggcg acgagttcac ggttctgctg
     2641 gagcagatca ccgacctcag cgacgtgatc cgggtcgccc agcgcattgc cgagcagtcg
     2701 ctgacgctct acaacatcgg cgatcgcgag gtctcggtga cagccagcat cggcatcgcg
     2761 ctgcccggtg acgacaacga gacggccgac gacctgctgc gtaacgccga catcgcgatg
     2821 tacgaggcca agcgacgcgg caaggcgcag tacgccgtct tctcggcgcc gatgaacgtc
     2881 cgcgcccgcg agcggctgga gcttgagatc gagctgcaac gggccatcga gcggggtgag
     2941 ttcgtcctcc actaccagcc tgtcgtcgat ctacggacgg gccggatcgc ggccgtcgag
     3001 gcgctgatcc gctggcggca cccgctgcgc ggactgctgc cgccggctga cttcatcgcg
     3061 atcgccgatg agaccggctc gatcctcgat atcggcggct gggtgctgcg acgggcctgc
     3121 cgcaagttgc agcagtggca ggaggagttc ccggccccgg ccggtcagcg acccttgatg
     3181 atgagcgtca acctctcgac gcgtcagttc ctgcaagcgt cactggtcga gacggtccgg
     3241 gaggtgctgg acgagaccgg gatcgagccg cgcacgctca agctcgaagt caccgagcgg
     3301 gtcatcatgg aggacgcgcc gtcggcgatc gccaccctgc gcgacctgcg cgagctcggc
     3361 atcacgctgg cgatcgacga tttcgggatc ggctactcat cgctgaccga cctgaagcag
     3421 ttcgccgtcg acctgctcaa gatcgaccgc acgttcgtcg atggcatcag ccaggacatg
     3481 gacgacacct cgatcgtcac cgccatcatc gcgatcggca agtcactcgg catccgcacc
     3541 gtcgccgagg gcgtcgagac acccgcccag ctcgcgttcc tgacgacacg cggctgcgac
     3601 agcggccagg ggcacctctt ctcgccacca ctcccaccgg aggagatcgc caccctgctc
     3661 cgcaaccaga ccaactacca gatcccccgc ccgatcacga gccgaccgct gacgttctac
     3721 aactcatagt catctattca cgatcaacgg gaagcatcac ccagccgtac tggacgaagt
     3781 gagcgtcaag gctgagtgcg tgtgtgattc caaggcactc catgaccgcg aaactggacg
     3841 catccgcgaa cgagaagtcc ttgtcggtga atcgatcgat gatctcccag gcacgctgct
     3901 cgtcatcgac actcacacgc acgaggctaa catcgtggct atcgcgaagg cgcgtgagtg
     3961 ccgtccgggc aacatgcctg ttgatgcggg aaagaagcag tgcgtgcaac tcggcgaaga
     4021 cgaagttagt cacgacgagc tgcccgccgg ctgcgacgat ttcctcagtt gctgcgacta
     4081 cggaactgtg aaaatcgtca tcgcgatcga ccatcgcgaa caacgccgag gtgtcaacaa
     4141 agaccaaacg gggatactga ggaaatctgc ggatcatcgg cgcttaacgt catatgcatc
     4201 agcaatgtac tcatctttat gatccgcgat actgcctgat gatcccgatg caccgagtcc
     4261 ggcgatatcc agaatcgaat gcggcttgtc acgattgaag cgttcaatgg gctcgattgc
     4321 atgcacgtga acttcgtaga caacgtcgcc ggtgttgatg aatatcggct tccgcgcttc
     4381 gctcgctgta cggagcgctt ccgccagttc gctgtcgacc cccgcatgaa tcatcgtgcg
     4441 ctctgcggcc atcgtgcgtg cctttctgca agtctcgaat cggctccgcc agataccagc
     4501 cagcggagaa tttctcgtgc tgcagtcgag tcctggtccc gtcgatcggg gatcgcgtag
     4561 taggcagagg tatcaacgaa gacgagtcga ggaacttcga tcgagcgagt tatcgaaggc
     4621 ggggatcgat cgcatcagca atatactggt ccttgaactc ggcaatattg gatggttcgc
     4681 gggattcgac gataccgatc aattcaagga tcgcgtcagg ctcatcagac gcgtccgcgt
     4741 tctcagcatc ccaccggaca cggatgtcga tctcatactg cgcatcgtca gtttcgatga
     4801 tgatggactc accacttgct gcggcctttc ggagcgtctg ggcgagttca ctttcagagt
     4861 tgatgtagac ggccattcgt gagatcgcca tgacggtcct ccgctgagta cacgtgcaag
     4921 ttcacacttc agtctgccat gtggcgaacc gtgctgctac cggcaactct cgaagacgcg
     4981 gcggacgatg gcgagggtgg cggcgtaggt ggagtccatg cctcggtagg acatgccacg
     5041 cgcgccaagg gtgtgggcgt gggagtaggg cgtgtccgac gcgtagtgga tgattccgat
     5101 gtcgatcggc acctggctca ggttgaccga ctcgctctgc gggtggcgcg tcaggtacga
     5161 gtcctcgtag agggcggaga ggaacgggcc ggcctccatc tcaacgacgg tgtagttctc
     5221 ccgatagtag aaatcgaggt agccccggtt ctgcaggtag gtgcctttga cggtcacggc
     5281 gcgctggtgg tcgagcgtcg agccgtagac gaggaacggc tccagatcga cgctggagaa
     5341 cgagttggcg aaccagtagg tgttgtcgga atgctcgtcg tagacgacat cggagatcat
     5401 cacgtcgccg atcctggcgt tcagggtcgc ggccttgccg aggacgtaga cgccgcgtag
     5461 ctgagcgact tcggccgaga cctggcagag gatgtggtag gccgccagcc cgagcgggta
     5521 gttgatgttg acgatgatgg cgtctgtgtc gggcggaccg ccgcagacct tgaggatgcg
     5581 cgggtcgaga tcagcggcgt cgagccggtc cagctcgatg atctggatgc cgacatcaag
     5641 cgcgctgtcc tgctcgatcg tcgtgatgcc acgctcttcc tcctcgcgat tgcggctggg
     5701 gcggtccgga ccgttggcgg tgtagtagag ccgggcggcg aagtagagga aattgtccca
     5761 cgacgagcgc gtgtcgccgg attgcagctt atgcagctcc ggcagcaggt ccgggtgctg
     5821 gctgtcctcg atgaaggcga tcagctcgtc gcgacggcga cgggcagtgc cgctcaggat
     5881 gttggcgatt gagtgcagat tcgaggagac gaaatagacg gggcgatcgg acaa
//
LOCUS       DASUGJ010000235         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_867591, whole genome shotgun sequence.
ACCESSION   DASUGJ010000235 DASUGJ010000000
VERSION     DASUGJ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867591"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..879)
                     /locus_tag="VFV93_05190"
     CDS             complement(328..879)
                     /locus_tag="VFV93_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5164771.1"
                     /translation="MRKIVAGLFIALDGVAESPDKWHFPYWSDEMGAAVGGQMANTDT
                     MLLGRVTYEEFAGYWPQQDADAEPFAAYMNNTPKLVVSNTLDTLDWQNSTLVSGDVYG
                     KLRELKAQPGKDISITGSISLTRSLLREGLLDELNLLVHPIVVGSGKRLWEGDTGNVP
                     LELAESKTLPNGVLHLIYRRAAG"
     gene            complement(964..1380)
                     /locus_tag="VFV93_05195"
     CDS             complement(964..1380)
                     /locus_tag="VFV93_05195"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5164772.1"
                     /translation="MRFMVLVKANEESEGGQMPTTEMLAEMGKYNEELVKAGVMLAGE
                     GLHPSSKGARVKFSGSNRTVTDGPFAETKELLAGYWIWQTGSLQEAIDWLKRAPFDGG
                     AEIEIRQIFEMEDFGEAMTPELREAEERMRRQTEGA"
     gene            complement(1523..1972)
                     /locus_tag="VFV93_05200"
     CDS             complement(1523..1972)
                     /locus_tag="VFV93_05200"
                     /inference="COORDINATES: protein motif:HMM:NF039977.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6069 family protein"
                     /protein_id="HEX5164773.1"
                     /translation="MSSIATYTSAPSVSRSTGRGRFALIGLATVVAAVLANVLFYYIG
                     NVFVTYKPDFVILANLGGTITMTAAAGIIAVLVYAALLRFSNNPVRVYAIVAAVVLVV
                     SIIPDFTMIPSEAGASTGQAVILALMHVVAAAVIVPMLTTLARPETR"
BASE COUNT          354 a          662 c          641 g          427 t
ORIGIN      
        1 cgcttctcgt cggatgtcag gatgaacgcg cccttgcgtg tgccgaccag gactcgtact
       61 ccgctcatcg ttttcgttgc tccatcctgc atgtcaagcg ctgcccgtag tccgggcatc
      121 cgccacatcg atcggcagaa ctgcgctgaa cgtctgttct accgaattgc acaagtgtac
      181 gtacactgtg tccgtctggc aactcactgt ctgagacgcg ctgtcgatct ctaccgatgg
      241 tgatgccccg tttcaggacg gcgatcgcgc atctcgccct gcgatcgtcg tcctgaaacg
      301 gggtaattcg ctggtggtgt cagtgtccta gccggctgcc ctgcggtaga tgaggtgcag
      361 gacgccgttg ggcagtgtct tcgattcggc gagctcgagc ggcacgttgc cggtgtcacc
      421 ctcccagagg cgcttgccgc tcccaacgac gatcgggtgg accagcaggt tcagctcatc
      481 cagcagcccc tcgcggagga gcgagcgggt gaggctgatg ctgccggtga ttgagatgtc
      541 cttgcctggc tgcgccttca attcgcggag cttcccgtag acatcgcctg agacgagcgt
      601 cgagttctgc cagtcgagcg tgtcgagcgt gttggagacg accagcttgg gcgtgttgtt
      661 catgtaggcg gcaaacggct cggcgtcggc atcctgctgc ggccagtaac cggcgaactc
      721 ctcgtaggtg acccggccga gtagcatcgt gtcggtgttg gccatttgcc cgccgacggc
      781 tgcgcccatc tcgtcgctcc agtacgggaa gtgccacttg tccggcgatt ccgcgacacc
      841 atcgagcgcg atgaacaatc cggcgacgat ctttctcatc tcggttcctt ccttcgttcc
      901 gtatcgtcgt cacaacgctg tgacagcagt gtgtcgatcg ccgtcgatcg acacgccccg
      961 ctcctacgcg ccctcggtct gcctgcgcat gcgctcctcg gcctcacgca gttccggcgt
     1021 catcgcctca ccgaagtcct ccatctcgaa gatctggcgg atctcgatct cggccccgcc
     1081 gtcgaacggc gcgcgcttga gccagtcaat cgcttcctgg agcgatccgg tctgccagat
     1141 ccagtagccg gccagcagct ccttcgtctc ggcgaacggg ccgtcggtca cagtccggtt
     1201 cgagccggag aacttgacgc gagcgccctt cgagctgggg tgcagccctt caccggcgag
     1261 catgacgccg gccttgacga gctcttcgtt gtatttgccc atctccgcca gcatctcggt
     1321 ggttggcatc tgcccgcctt cggactcctc gttcgccttg accagaacca tgaatcgcat
     1381 ctgtttgtcc cttctacctg atcgtgttcg gcaaccggac gacggtggcc ggctgccgag
     1441 ctcaacgtcc actcgcgata tagtcgatcg gtggggccgg ttttcgacaa ccggtcccac
     1501 cgaattcgaa cccatcgatc gattagcgcg tctccggccg cgccagcgtc gtcagcatcg
     1561 gaacgatgac ggcagcggcg acgacgtgca tcagcgcgag gatcacggcc tgaccggtgc
     1621 tggcgccggc ttccgatggg atcatcgtga agtccgggat gatcgagacg accaggacga
     1681 cggccgcgac gatcgcgtag acgcgaaccg ggttgttgct gaagcgcagc agcgcggcgt
     1741 agacgaggac ggcgatgatg ccggcggcgg ccgtcattgt gatcgtcccg ccaagattgg
     1801 cgaggatgac gaaatcgggc ttgtaggtga cgaacacgtt cccgatgtaa tagaagagaa
     1861 cgttcgcgag aactgccgcg acgacggtcg cgaggccgat gagagcgaag cgtccccggc
     1921 cggtcgagcg ggaaaccgaa ggcgctgacg tgtaggtagc gatcgacgac attgtggacc
     1981 atccctttcg tgcatcttgc acctgctccg gtaaccagcc acttggctgt tacaccaggt
     2041 agtcgtctgg cgatgcgcga aatcgacaac tgctccacac gaat
//
LOCUS       DASUGJ010000236         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_588417, whole genome shotgun sequence.
ACCESSION   DASUGJ010000236 DASUGJ010000000
VERSION     DASUGJ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588417"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1488)
                     /locus_tag="VFV93_05205"
     CDS             complement(166..1488)
                     /locus_tag="VFV93_05205"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5164774.1"
                     /translation="MVRRTETTQQQREEIEGVLPGETATARAAHASLTNRRRGFAKIL
                     PFLGPAFVASVAYVDPGNFATNVSAGAQFGYLLLWVVLSANLMAMLVQAMSAKLGIAT
                     GMNLAEVCREQFSPMTSRLLWVQAEIVAIATDLAEFVGAALALNLLFGIPLFLAGLLT
                     AVATFATLEMQRRGFRPLEAVISSMVGVIVFAFALQVFYAKPEVDKVLAGMFTPQFSG
                     TESVLLATGILGATVMPHVIYLHSALTQGRIVGRTDDEKRRIFRFEMIDVVIAMGIAG
                     LVNMSMLSIAAALFHSRGLLHIEDIDEVFNQLKLTEGNGMAILFGVALLASGLSSSSV
                     GTMAGQVVMQGFIQRHIPIFLRRAVTITPALIVLGAGINPTDALIISQVVLSFGIPFA
                     LIPLLMFCRNRDIMGSLVNHRLTTSVAILVVTLIIGLNGFLLYETILG"
     gene            complement(1628..2845)
                     /locus_tag="VFV93_05210"
     CDS             complement(1628..2845)
                     /locus_tag="VFV93_05210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164775.1"
                     /translation="MESEHSRTAQSARRALLLSVYVPTALLSLAEGILLPTLPLYALD
                     FDVSFAVASLVLSAAGIGTLIADVPAGMLLGRMGLKPTMLLGAGMVIFGTFMIGLAGS
                     IEQIIAFRLISGVGTALWGLSRHAFITESIDPRQRGRAISVFGGINRIGMFGGPAVGG
                     VIGTAFGLSASFFVAAGLGMVGLVVSYFYVKDTRVVVHSDRGMRWGLVRNMIRTNWRD
                     LGAASIAQTFAQLIRSGRQTIIPFYGASVLGLGVAEVGLIQTASSSVDMLLFIPAGLL
                     MDHFGRKVAAVPSFAIMGVGMVLIPFATSYIMLMGAAVLIGIGNGLGSGSMMTLGADL
                     APSGATGEFLGLWRLIGDSGRATGPLAVGSLTDSLGFEYTAYILAGFGVGAALILAAL
                     VKETRVVEVRSTG"
     gene            3061..>3244
                     /locus_tag="VFV93_05215"
     CDS             3061..>3244
                     /locus_tag="VFV93_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5164776.1"
                     /translation="MAAIELEHVGIPAPGASFDPTVQFYAKVFGMQIIREVNAGQHLA
                     FLSDGKGGRIEVLDTEG"
BASE COUNT          615 a         1082 c         1051 g          496 t
ORIGIN      
        1 gacctgctga ttgttgatgg tgatcccctg gcagacatct cgattctcga tcggcaggaa
       61 tgcatccagc tggtgatgaa gaacggccgg acctaccgga atacgctgcc tgcgccgtcg
      121 cccgccgaga tcgcggcggc atcctgaggg agtgttgacc tgcgactacc ccaggatcgt
      181 ctcgtagagc aggaacccgt tcaggccgat gatcagcgtg acgaccagga tggcgacgga
      241 cgtggtcagg cggtggttga cgagtgagcc catgatgtcg cggttgcgac agaacatcag
      301 cagcgggatc agcgcgaagg ggatgccgaa cgagaggacg acctggctga tgatcagcgc
      361 atcggtcggg ttgatgccgg cgccgaggac gatcagggcc ggcgtgatcg tcaccgcccg
      421 ccgcaggaag atcgggatgt gccgctggat gaagccctgc atgacgacct gaccggccat
      481 cgtcccgacc gacgagctgg agaggccgct ggccagcaac gcgacgccaa acaggatcgc
      541 catcccgttg ccctcggtca gcttgagctg gttgaagacc tcgtcgatgt cctcgatgtg
      601 gagcagcccg cgcgagtgga acagcgccgc cgcgatgctg agcatgctca tgttgaccag
      661 cccggcgatg cccatcgcga tcacgacatc gatcatctcg aagcggaaga tgcgccgctt
      721 ctcatcatcg gtccggccga cgatccgtcc ctgtgtcagg gcggagtgca ggtagatcac
      781 atgcggcatc accgtcgcgc cgaggatacc ggtcgccagc aggacgctct cggtgccgga
      841 gaactgcggc gtgaacatcc cggccaggac cttgtcaacc tccggcttgg cgtagaacac
      901 ctgcaacgcg aaggcgaaga cgatcacacc gaccatcgat gagatgacgg cctccagcgg
      961 tcggaagccg cgccgttgca tctccagcgt cgcgaaggtc gccaccgccg tcagcaggcc
     1021 ggccagaaag agcgggatgc cgaagagcag gttcagcgcc agcgccgccc cgacgaactc
     1081 ggccaggtcg gtcgcgatcg cgacgatctc ggcctggacc cacagcagcc gtgaggtcat
     1141 cggcgagaac tgctcgcggc agacctcggc cagattcatg ccggtcgcga tgccaagctt
     1201 ggccgacatc gcctggacca gcatcgccat caggttcgcg ctgaggacaa cccacagcag
     1261 cagatacccg aactgcgccc cggccgagac gttcgtcgcg aagttgccgg ggtccacata
     1321 ggcgaccgac gccacaaatg ccggccccag aaacggcaga atcttcgcga acccccgccg
     1381 ccggttcgtc aacgaggcat gcgccgcccg tgccgttgcc gtctcccccg gtaatacccc
     1441 ttctatctcc tcgcgttgct gctgcgtcgt ctctgtgcgt ctcaccacgc caagcccaat
     1501 ccactcttta gcccagccta atcctaagac cggacgtgtg agaatgcaat tgggtgttgc
     1561 gggacgcggc atcgttccgg tgtaggggcc gtggctccgt cccacgtccc gcgtcccgcg
     1621 tcccgattca ccccgtactc cgcacctcca ccacgcgggt ctctttgacg agcgctgcca
     1681 ggatgagggc ggcgcccacg ccgaagccgg ccaggatgta ggcggtgtac tcgaagccga
     1741 ggctgtcggt caggctgccg acggcgagtg gaccggttgc ccggccagag tcgccgatca
     1801 gcctccagag gccgaggaac tcgcccgtcg cgccggatgg ggccaggtcg gcgccgagcg
     1861 tcatcatcga gccgctgccg aggccgttgc caatcccgat caggacggcc gcgcccatca
     1921 gcatgatgta gctggtggcg aacgggatca gcaccatccc gacgcccatg atcgcgaacg
     1981 acgggacggc ggccactttg cggccgaagt ggtccatcag caggccggcc gggatgaaca
     2041 gcagcatatc gaccgacgac gaggccgtct ggatcagtcc aacctcggcg acccccagcc
     2101 cgaggaccga tgcgccgtag aacgggatga tcgtctgccg gccggagcgg atcagttgcg
     2161 cgaaggtctg ggcgatgctg gccgcgccga ggtcgcgcca gttggtgcgg atcatgttgc
     2221 ggaccagccc ccagcgcatc ccacgatcgc tgtggacgac gacccgcgtg tccttgacgt
     2281 agaagtacga gacgaccagc ccgaccatcc ccagcccggc cgcgacaaag aacgaggccg
     2341 acaggccaaa ggccgtgccg atcacgccgc caacggccgg cccgccgaac atgccgatcc
     2401 ggttgatgcc gccgaagacg gagatcgccc ggccgcgctg gcgcgggtcg atcgactcgg
     2461 tgatgaaggc atgccgcgag agtccccaga gggcggtccc gacgccggag atgagccgga
     2521 aggcgatgat ctgctcgatg ctgccggcca gcccgatcat gaaggtgccg aagatcacca
     2581 ttccggcgcc gagcagcatc gtcggcttca gccccatccg ccccagcagc atcccggccg
     2641 gcacgtcggc gatcagcgtg ccgattccgg ccgccgagag gacgaggctg gcgacggcga
     2701 aggagacatc gaagtcgagc gcgtagagcg gcaacgtcgg cagcaggatg ccttccgcca
     2761 gcgacaggag cgcggttggc acgtagacag acagcagcag cgcccgcctg gccgactgtg
     2821 ctgtacgact atgttcgctt tccatcagcc tcgttttgtt tcgggattcg ggattcgggg
     2881 agatcgcacg tctcatcttc cgatcgacgt gctcggcgcc ctgcgcccaa atcccaaatc
     2941 ccgcatcccg gttcccgccc ccaacgtccc ggaaccagcg ccggtcatcc taacacgctc
     3001 gtcacatggc gggtatcatg cgcgggtcga atgatgcgtg acgctgaatg gaggagcaag
     3061 atggcagcga tcgagcttga gcacgttggt atcccggcgc cgggcgccag cttcgaccca
     3121 accgtccagt tctacgccaa agtcttcggg atgcagatca tccgcgaggt gaacgccggc
     3181 cagcacctgg ccttcctttc ggacggcaag ggcggccgga ttgaggtgct ggacaccgaa
     3241 ggct
//
LOCUS       DASUGJ010000237         5268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1283890, whole genome shotgun sequence.
ACCESSION   DASUGJ010000237 DASUGJ010000000
VERSION     DASUGJ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283890"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFV93_05220"
     CDS             <1..352
                     /locus_tag="VFV93_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5164777.1"
                     /translation="AAYLLPRVVGQRRALELLLFGDAIDAETAANYGIANRVLPHDDL
                     LPAAYEWARRLADGPSLAIAMTKRMVNNEWNMDMVSALEAEAQAQALLMMGDDHRIFH
                     EAFREKTQPKFSGR"
     gene            517..1047
                     /locus_tag="VFV93_05225"
     CDS             517..1047
                     /locus_tag="VFV93_05225"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX5164778.1"
                     /translation="MAGDPHNEVAVLDYQPGWVAVFDAERERIAAALALVVPRPSAIE
                     HVGSTAVAGMAAKPIVDIMVTYARMPAVDEVAAALAAIAYELVDKPEFTESLFFRRGE
                     TLATTIHLHVTTETSAFGRQMLRFRDALRASPRLAAEYAAFKRELAARFPDDRPSYTQ
                     AKGPFISGIIGEPPSS"
     gene            1118..2272
                     /locus_tag="VFV93_05230"
     CDS             1118..2272
                     /locus_tag="VFV93_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5164779.1"
                     /translation="MHFELTDQQRAIQARARAFADDEVAPLARAADETGRFPLELVPR
                     MGDLGFLAGPLAAEYGGSGMDNLSFALVSEELGRVDSSVRGFVSVHAGLVSLCIEHWG
                     SEEQKRRYLPQLATGALIGCYCLTEPNAGSDAAAMESTCHEDADGWVLNGEKIWITNA
                     PDAHLAIVFATMDRSLRHRGICAFLVPTDTPGFRREPMPGHELGHRAASHSHVYLENC
                     RVPKDALLGEPGQGFRVAMSALDDGRLGVAAGAVGVAQACLDASIAFTRERHAFGQRV
                     ADFQMIQATLADMAADVEAARLLVYRAAWVKDQGRPSTKETSIAKLFATEAAVKIASD
                     AVLLHGGRGYSSAYSVERYYRDIKGLQIYEGTSHIQRIVIARELVGRPER"
     gene            2395..2856
                     /locus_tag="VFV93_05235"
     CDS             2395..2856
                     /locus_tag="VFV93_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164780.1"
                     /translation="MTQRLKTAGLALAILVLGIGLTVAVTEYAAGADTTRPSLLTSSD
                     VVQALAEQGVEVGSESLPAHHPLLEIAGTAYTAGSATIEVYVYPSVESRVADEQLIQR
                     QLMHLQAFEGGGDQLPRVTSARNVLLLFNADPHGSATPIFAAARALISGAD"
     gene            2972..3310
                     /locus_tag="VFV93_05240"
     CDS             2972..3310
                     /locus_tag="VFV93_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5164781.1"
                     /translation="MSEWLFLTNQARVLLYIAQNPASTARKIAQAVGITERATQRLIR
                     DLDDAGYITRTRVGRHNEYVIHRDQPVSGEVIGDVPVSALIDCLMCEAPSSALRRPMP
                     DARSEASVSD"
     gene            complement(3307..4482)
                     /locus_tag="VFV93_05245"
     CDS             complement(3307..4482)
                     /locus_tag="VFV93_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5164782.1"
                     /translation="MRRFILIIIATAVLAAAIFYVDFSTLWSALAGLSTTTIVLLAVL
                     LLAGALVKSLRWAFFLRAADLDISWRDGMTTYLAGMAAGAIPGGSWLPARLAQEHGSV
                     RMRQAVSGLFVGFVADMIALAILAGTMILVMGEPRPRLLIPVAAIALAILLIAMGRSE
                     RLWYFIDRLLARSRFTSGLLPKEADIHQKISAVMRTTVVGRGVGFSLMTTTVSVCILY
                     LLVNGLTFTGISVRQAYYVHSFSESAATVIPSPGGIGVIDSSMAGLLNSLSIGWVRAT
                     FVVLTIRSIDLLFKTVFGSVMLVVFYHRLLRDVLQFKRRARRVGLGAWRAWRFSWRFA
                     TRQNGTPSHSATTVQETRQTPPPSSRDNFQTTGVISANTTTHPANRVHPSAHHGSPD"
     gene            complement(4577..5074)
                     /locus_tag="VFV93_05250"
     CDS             complement(4577..5074)
                     /locus_tag="VFV93_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164783.1"
                     /translation="MPGFILTHPALSAIVMASLGILGNWAIVRNVANWPPWMLFPSMI
                     VAVVCLLTMVIGLGILTVRLLEPALALMDLNWDAEPLAQLGIPLSLQRKCERLGYWTA
                     EDLVRDIDTGKFPWTLIEYDERMQLERAAARWNAQPGTGKRRRGLLSRRQPAANDLPA
                     NDRGR"
BASE COUNT          955 a         1693 c         1737 g          883 t
ORIGIN      
        1 agctgcctac ctgttgccac gggtcgtcgg ccagcgccgg gcgctggaac tgttgctgtt
       61 cggggatgcc atcgacgctg agacggcggc gaactacggc atcgcgaacc gcgtcctgcc
      121 gcacgacgat ctgctgccgg ccgcctacga gtgggccaga cggctggcag atgggccgtc
      181 gctggcgatc gcaatgacga agcgcatggt caacaacgag tggaacatgg acatggtctc
      241 ggcgctggaa gccgaagcgc aggcccaggc gctgctgatg atgggcgatg accaccgcat
      301 cttccacgag gcattccgcg agaagacgca gccgaagttc tccggacgtt gacatacccg
      361 ttgacagatg gcggttcgca ccctaccgta tcgctatcct gacatccggg ttcgccagca
      421 ttgggagagg aatccgccgt ggaacggcct gcccacacgc tccggattgg cgcgtatcgt
      481 cgccaactgc cggccacggg cggcaggtag gtcgcgatgg ctggcgatcc gcacaacgag
      541 gtggcggtcc tcgactacca accgggctgg gtagccgtct tcgatgcgga gcgcgagcgt
      601 atcgctgctg cgctggcgct ggttgttcca cggccgtccg cgatcgagca tgtcggcagc
      661 acggccgtcg ccgggatggc ggccaagccg atcgtcgaca tcatggtcac ctacgcgcgc
      721 atgcctgccg tggacgaggt cgcggcggcg ctggctgcga tcgcctatga gctcgtcgac
      781 aagccggagt tcacggagag tctcttcttc cggcggggtg agacgctggc aacaactatc
      841 cacctgcatg tgacgaccga gaccagtgcg tttggacgcc agatgctgcg ctttcgggat
      901 gcgctgcgcg cctcgccgag gcttgcggcc gagtacgccg ccttcaaacg ggagcttgcc
      961 gcgcgcttcc ccgacgaccg gccctcgtat acacaagcga agggtccctt catctccggc
     1021 atcatcggcg aaccgccatc gtcctgacca ccgtcgatgg cgcgactaca atcctgatgc
     1081 cggtgagtgg ttccgctgaa cgacgaggga gagactgatg cactttgagt tgaccgacca
     1141 gcagcgtgcg attcaagcgc gggcacgggc gttcgccgac gacgaagtcg ctccgctggc
     1201 gcgtgctgcg gatgagaccg gccgcttccc gctggagctg gttccgcgca tgggcgacct
     1261 cggtttcctg gccgggccgt tggcggctga gtatggcggc tcggggatgg acaatctctc
     1321 gttcgcgctc gtctccgagg agcttgggcg ggtcgactcg tcggtgcgtg gcttcgtctc
     1381 ggtccacgcc gggctcgtct cgctctgcat cgagcactgg ggcagcgaag agcagaagcg
     1441 tcgctacctg ccgcagctgg cgaccggcgc gctgatcggc tgctactgcc tgaccgagcc
     1501 gaacgccggg tccgacgccg ccgcgatgga gtcgacctgc catgaggatg ccgacggctg
     1561 ggtgctgaac ggcgagaaga tctggatcac gaatgcgccc gatgcccatc tcgccatcgt
     1621 cttcgcgacg atggaccgct cgctgcgtca tcgcggcatc tgcgccttcc tcgtcccgac
     1681 cgacacaccg ggcttccgcc gcgaaccgat gccgggtcac gagctcggac accgcgccgc
     1741 cagccactct cacgtctacc ttgagaactg ccgggttccg aaggacgcgc tgctcggcga
     1801 gccggggcag ggcttccggg tcgccatgtc agcgctggat gacggccggc tcggcgtcgc
     1861 agccggcgcg gttggcgtcg cccaggcctg cctggatgcc agcatcgcct tcacccgcga
     1921 gcggcacgcg ttcggccagc gggtggcgga tttccagatg atccaggcga cgctagccga
     1981 tatggcggcc gatgtcgaag cggcccggct gctcgtctac cgtgctgcct gggtcaagga
     2041 tcaggggcgg ccatcgacca aggaaacgtc tatcgccaag ctgttcgcga ccgaggcagc
     2101 ggtcaagatc gcgtccgacg ccgtcctgct ccatggcgga cggggctact ccagcgccta
     2161 ctcggtcgaa cgctactacc gcgatatcaa ggggctccag atctacgagg gcaccagcca
     2221 cattcagcgg atcgtcatcg cccgcgagct ggtcggccgg cccgagcgct gacccgatct
     2281 gcacctctca cggaacaatc acgacgcctc cgacgttgag tcaacagcgc ggacaacgtc
     2341 gtccgcgccg ccggctcaat ggggagaccc ggcaggggac gagaggcgat agtgatgact
     2401 cagcgactca aaacggccgg gctggcgctg gcgattctgg tgctcgggat cgggctgacg
     2461 gtggccgtga cggaatatgc ggccggcgcg gacacgaccc ggccgagcct gctgacgagc
     2521 agcgacgtcg ttcaggcgct ggccgaacaa ggcgtcgagg tcggttctga gagcctcccc
     2581 gcccaccatc cgctgctgga gatcgccggc actgcctaca cggccggttc ggcgacgatc
     2641 gaagtctatg tctatccctc ggtcgagagc cgggtcgccg acgaacagct gatccaacgc
     2701 cagttgatgc acctacaggc gttcgaaggc ggcggtgacc agctaccccg cgtcacctcg
     2761 gctcgcaacg tgctattgct gttcaacgcg gaccctcacg gctctgcaac ccccatcttc
     2821 gcggctgccc gcgcactcat ttcaggggct gattgagcgc cagttcgtat cgtgattcgt
     2881 ggaacagagt ttgcgtttgt ccagcatggc cgaccgacac gcgccgggct ggcgctggtg
     2941 gctgacgcag agaggcggga gacaaactgc tgtgagcgaa tggctattcc taactaatca
     3001 ggcgcgcgtg ctcttgtaca tcgcccagaa tccggccagt acggcccgca agatcgccca
     3061 ggcggtcggg attaccgaac gcgcgacgca acggctcatc cgggacctgg atgacgccgg
     3121 ctacatcacg cgaacgcggg tcggccggca taacgagtat gtcattcatc gcgatcagcc
     3181 ggtcagcggt gaggtcattg gtgatgtgcc ggtgtcggcc ttgatcgact gcctgatgtg
     3241 cgaagcacca tcaagcgcgc tacggcggcc gatgccagac gcacgttccg aagccagcgt
     3301 ttcggactag tcaggcgatc cgtgatgggc agagggatgc acccggttgg ccgggtgcgt
     3361 cgtcgtgttt gccgagatca cgcccgttgt ctggaagttg tcccggctgg acggcggcgg
     3421 ggtctgtcgg gtttcctgaa cggttgtcgc cgagtgtgag ggtgtaccgt tctgtcgtgt
     3481 tgcaaaccgc cagctgaatc gccacgcccg ccaggcgccg agtcccacgc gcctggctcg
     3541 ccgcttgaat tgaaggacat cacgcagcag gcggtgatag aagaccacca gcatcaccga
     3601 accgaagacg gtcttgaaaa gcaggtcgat cgagcggatc gtcagtacga cgaacgttgc
     3661 ccggacccag ccaatcgaga gcgagttcag tagccccgcc atcgacgagt cgatgacgcc
     3721 gatcccgcct ggcgagggga tcaccgtcgc ggccgactcg gagaacgagt ggacgtagta
     3781 ggcctgccga acgctgatgc cggtgaacgt caggccgttg accagcagat acagaatgca
     3841 gacggacacg gtcgtcgtca tcaggctgaa tccgacgccc cggccgacga cggtcgtgcg
     3901 catgacggcc gagatcttct ggtgaatatc ggcttctttc ggcaacaggc cactggtgaa
     3961 gcgcgagcgg gccagcaggc gatcgatgaa gtaccagagc cgttcggagc gccccatcgc
     4021 gatcagcagg atcgccagcg cgatcgcggc gacggggatc aacaggcggg gccggggctc
     4081 gcccatcacg aggatcatcg tcccggccag gatcgccagc gcgatcatgt cggcgacgaa
     4141 gccgacgaac aggccggaga cggcctgacg catcctgacg ctgccgtgct cctgtgccag
     4201 ccgcgccggc aaccatgagc cgcccggaat cgctcccgca gccataccgg ccagataggt
     4261 tgtcatccca tcgcgccagc tgatgtccag atcggctgcc cgtaggaaga atgcccagcg
     4321 aagcgacttg accagtgcgc cagccagcag cagaacagcc agcagcacga tcgtcgtcgt
     4381 cgaaagaccg gcaagggcag accacagcgt cgagaaatcg acgtagaaaa tcgcggcggc
     4441 aagcaccgcg gttgcaatga tgatcagaat gaaccggcgc accagcgtct ggcctcttgc
     4501 aaatccggag cggcggcgtc cctgccgagc tgcctcatcc ggacatacct gacttgctaa
     4561 tctagattat gacacgttag cgtcctcgat cgttggcggg caggtcattg gcagccggtt
     4621 gccgacgaga caggaggccg cgacggcgct tgcccgtgcc cggctgggcg ttccaacgtg
     4681 cggcggcgcg ctcaagctgc atccgctcgt catattcgat taacgtccag ggaaacttgc
     4741 ccgtgtcgat gtcccgaaca aggtcttcgg ccgtccagta gcccagccgc tcacacttgc
     4801 gctgcagcga caacggtatc ccaagttgcg cgagcggttc ggcgtcccag ttgaggtcca
     4861 tcaacgccag cgccggttcc agcaggcgaa cggtcagaat gccgagtccg atcaccatcg
     4921 tcagcaggca cacgacagcc acgatcatgc tcggaaagag catccacggc ggccagttgg
     4981 ccacgttgcg gacgatggcc cagttgccga ggatgccaag gcttgccatg acgatcgcag
     5041 acagcgccgg gtgggtcagg atgaagccgg gcacgcgcgg cgtaaatggc tcgtggcgcc
     5101 ggtcgcggtc gagcttgttg tgatcgagcc agtgctgggg tccctgaaac cagtgcacag
     5161 tcgcggtgcc tccttgcacg cgggcagcta cgagcgtcct gtcgacgtgg ttgcatggat
     5221 cgtaccgtgc ctcgacagtg tagcaacctg ttcctgaata caacacac
//
LOCUS       DASUGJ010000238         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_728236, whole genome shotgun sequence.
ACCESSION   DASUGJ010000238 DASUGJ010000000
VERSION     DASUGJ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728236"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..779)
                     /gene="tatC"
                     /locus_tag="VFV93_05255"
     CDS             complement(33..779)
                     /gene="tatC"
                     /locus_tag="VFV93_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX5164784.1"
                     /translation="MSPDLDEDVFVEMTLADHLEELRSRILKSAIAVGVCLIAAFWLS
                     WRVLDAIADSANVSNDQMALIGPTEGFVTWFKVVFYIAIALAMPVLIYQLMAFLVPGL
                     TRRERRYVIRAVPFVFIMFCAGAAFAFFVLAPRMLQWLSTFGIDYFRWDPRASEIVQF
                     YMTLMLGMGLIFELPVVMYALALIGIMSAKRYGRFRKYALILGMIAAAIITPTPDPFN
                     MMLVAVPTYALYEIGILLSRTVRKSLRSQE"
     gene            complement(1134..1373)
                     /gene="acpP"
                     /locus_tag="VFV93_05260"
     CDS             complement(1134..1373)
                     /gene="acpP"
                     /locus_tag="VFV93_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5164785.1"
                     /translation="MSAVYDRLQAIVAEQLGVEPEKITPDAEFIQDLNADSLDMVELV
                     MSLEEEFGVEISDEEVEKIVKVSDAVEYIEENANA"
     gene            complement(1665..2114)
                     /gene="nusB"
                     /locus_tag="VFV93_05265"
     CDS             complement(1665..2114)
                     /gene="nusB"
                     /locus_tag="VFV93_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5164786.1"
                     /translation="MSSRKIRAAENHQARTLALQILFETDLTGHAIEGVLRRYSEDMA
                     LPEPVRHYTERLVNGVNARLIEIDDRIGVAAPAFPVGQLPAVDRNILRVAIYELAHEP
                     EIPFKAAINEAVELAKLFGGDNSSRFVNGVLGTIAAQQKRDSPESDV"
     gene            complement(2159..>2702)
                     /locus_tag="VFV93_05270"
     CDS             complement(2159..>2702)
                     /locus_tag="VFV93_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase"
                     /protein_id="HEX5164787.1"
                     /translation="FIEANTRVQVEHPVTEAITGIDIIGWQIRVAAGEPLPFSQGDVK
                     LNGHAIECRVNAENAANGFSPSTGIIEGYVAPGGPGIRVDSHLYSGYTVPPNYDSLVG
                     KIIAWGPTREEAVARMRRALSETLIVGIETTIPFYSYVMAQPEYTSGKLDTSMIADFV
                     ERMGASLTTGSEPVLANAGS"
BASE COUNT          512 a          878 c          817 g          495 t
ORIGIN      
        1 caccgtggtg gaccactcag gacgacaggg ggttactcct gcgagcgcaa tgacttgcgg
       61 acggttcgcg agagcaggat gccgatttcg tagagtgcgt acgttgggac agcaaccagc
      121 atcatgttga agggatccgg cgtcggcgtg atgattgcgg ctgcgatcat gccgagaatc
      181 agcgcgtact tgcggaagcg cccgtagcgc ttggcggaca tgatgccgat caacgccagc
      241 gcgtacatga cgaccggcag ctcgaagatc aggcccattc cgagcatcaa cgtcatgtag
      301 aactgcacga tctcggatgc gcgtgggtcc caacggaaat agtcgatgcc gaacgtcgac
      361 agccactgga gcatacgggg ggcgaggacg aagaatgcga acgcggcgcc ggcgcagaac
      421 atgatgaaga cgaacggaac cgcgcgtatc acgtagcgcc gttcgcgcct cgtcaggccc
      481 ggcaccagaa atgccatcaa ctggtagatc agcaccggca tcgccagcgc gatggcgatg
      541 tagaagacga ccttgaacca ggtaacgaag ccctcggtcg ggccgatcag cgccatctga
      601 tcgttcgaga cgtttgccga gtcggcgatc gcgtccagga cgcgccagga gagccagaag
      661 gccgcgatca gacagacccc gacggcgatc gccgacttga ggatgcggct gcgcagttcc
      721 tcaagatggt cggccagcgt catctcaacg aagacatcct cgtcgagatc cggggacatg
      781 gggtcgtcag cggtcggggg ttgcgcctga ttggcgcccg agcggcgccc acgtatcggg
      841 gacgcgacgc ggttgatgat gcttgccaat cgccgtctca cgttctcaga taacgatggc
      901 cgctggccgg agaatcgcgg tcctcggaca gcaggccggc gcgcacatcg cgcctgctcg
      961 cgacagccgg gcattcagtc acagggatcg tcagcatgcc gcgtgccgtg tgattgtatc
     1021 gctcgccgcc ggaacggcgc aaaaatcgga gcgccgggcg cgcaacgacg ctcccggcgc
     1081 tctccctgcg tcttcatgta cggccggaac gctacaaccg gccctatcgc cgatcaggcg
     1141 ttggcgttct cttcgatgta ctcgaccgcg tcgctgacct tgacaatctt ctcgacctcc
     1201 tcgtcggaga tctcgacgcc gaactcttct tccagcgaca tgaccagttc gaccatgtcg
     1261 agcgagtcgg cgtttagatc ctggatgaac tccgcgtccg gcgtgatctt ctccggctcg
     1321 acgccaagct gctcggcaac gatggcctgc aaacgatcgt agactgctga cactacgggt
     1381 acctccccct tcgatcaaaa cgtcgtccac agggacgtgc gccgccgccg atcatcgtcg
     1441 cgtctcgcgc gccccaagcg ggatctggca atcgcgccag ttacccattc ctcggccggc
     1501 gcccccaccg gcacgtcacc tgtgttcttc gccgcgttcg gctcactata cgtcaaaacg
     1561 ctacccggcg cgacgccctc tgcggcgttc ggtgccgcca ggtcagtcgt cgttcgcctg
     1621 ccgcgtccgg cgaattatgc atgacaccca tgcacgccac aaaactatac gtcgctctcc
     1681 ggcgaatcgc gcttctgctg agcggcgatc gttcccagga cgccgttgac gaaccggctc
     1741 gaattgtcgc cgccaaacag cttcgccagc tcgaccgcct cgttgatggc cgccttgaac
     1801 gggatctccg gctcgtgggc aagttcgtag atggcaaccc gcaggatgtt gcgatcgacg
     1861 gccggcaact gcccaaccgg aaacgcgggc gcggcaacgc cgattcgatc gtcgatctcg
     1921 atcagccggg cgttgacacc gttcaccagc cgctcggtgt agtggcggac aggctccggc
     1981 agcgccatat cctcggagta gcgccgcagc accccttcga tggcgtgacc ggtcaagtcc
     2041 gtctcgaaca ggatctgcaa tgccagtgtg cgtgcctgat ggttctcggc cgcgcggatc
     2101 ttacgtgaag acattcgtct gctttcagcc ttcgtcactt catcgtctgc tgaaccaact
     2161 acgagccggc gttcgccagc actggctcgg acccggtcgt caggctcgcg cccatccgct
     2221 cgacgaagtc ggcgatcatc gacgtatcga gcttgccgct cgtgtattcg ggctgggcca
     2281 tcacatagct atagaacggg atcgtcgtct caatcccgac gatcagcgtt tcagacagcg
     2341 cccgacgcat ccgcgcgacg gcctcctcgc gggtcggccc ccaggcgatg atcttgccca
     2401 ccagcgaatc gtagttcggc gggacggtgt agccggaata gagatgcgaa tcgacacgaa
     2461 tacccggccc tcccggcgcg acatagcctt cgatgatgcc ggttgatggt gaaaagccgt
     2521 tggcagcgtt ttcggcgttg acgcggcact cgatcgcgtg tccgttcagc ttgacgtctc
     2581 cctggctgaa cggcaacggc tcaccggccg caacccgaat ctgccagcca atgatgtcga
     2641 tccccgtaat cgcttccgtc accggatgct caacttgaac acgcgtgttg gcttcgatga
     2701 ag
//
LOCUS       DASUGJ010000239         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_450912, whole genome shotgun sequence.
ACCESSION   DASUGJ010000239 DASUGJ010000000
VERSION     DASUGJ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450912"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2052)
                     /locus_tag="VFV93_05275"
     CDS             complement(<1..>2052)
                     /locus_tag="VFV93_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5164788.1"
                     /translation="HDNSLYSIAWGAPIGERLHLLLSGEYYQADGVDSYAERSWYQGN
                     SYINNPDTSNATPRYLLRPDVVSAVATNGGLINSGVPTTSALYRRFFQPDGSLAPFVM
                     GEGTPTHAHSITNGGSGDDTTGDLLALSPEAERYSAFAYLSIDATPDLNFYVQALGGQ
                     SMTDQPDHGGRFAAIAGLDTRITIFRENAFLPAAVRQIMVDEGLNSFQMNYVGDRDGL
                     GRTSRLKQDNDTTSGTAGFKWDLGDDWQMNGYVQSGKADNRGYQQGVLLDRMMAAVDA
                     VVDPSGNIVCRAATINPGKWGDCVPLNLFGRGNASDAAISYVTNFTPGQQITSPLFFQ
                     PDEYESGQTISYTSGVGKVYNTETRQKVIDLSASGRLWDGWAGPIVAAFGAGWRREEL
                     EQIVWDPSNPASDPNIFPASAALAPELRAVPSNIATRSSMIQNSTVANVHGHYVVKEA
                     FTEWQVPLLRDKGWAEDLSLLAAARAAEYTGSGNVWSWKWGLDWRLNSALRFRGTVSQ
                     DVRAATLLERFNQTGGVGTVTRDPMFPNDGAQAISTRTGGNPDLDPETSHTTTYGFVY
                     QPTRLENFSLSVDYWDVDIDGAIGNLGIQRIVDDCFSGASNVCGLVTRDPVTQRLAQV
                     RNITQNISAAAGRGVDVEVGYRWQNLGARLFWSKMKENSTMTDRSNPTTYFDSAGQTG
                     IG"
BASE COUNT          342 a          634 c          680 g          396 t
ORIGIN      
        1 gccgatgcca gtctgaccgg ccgagtcgaa atacgtcgtg gggttgctgc ggtcggtcat
       61 cgtggaattc tccttcatct tcgaccagaa caaccgcgcg cccaggttct gccaccggta
      121 gccgacttcc acgtccactc cgcgcccggc tgcggccgag atgttctgcg tgatgttcct
      181 gacctgtgcg agacgctgtg tcaccgggtc gcgcgtgacg agcccacaca cgttcgacgc
      241 gccgctgaag caatcgtcga cgatgcgttg gattccgagg ttcccgatgg cgccgtcgat
      301 atcgacgtcc caatagtcga cggagagaga gaagttctcg agccgggtcg gctgatatac
      361 gaaaccgtag gtggtggtgt gggatgtctc cgggtcgagg tctggattgc cgccggtgcg
      421 cgtcgagatc gcctgtgccc cgtcgttggg gaacatcgga tcccgcgtga ccgtcccgac
      481 tccgccggtt tgattgaaac gctcgaggag cgttgcggcc cggacgtctt gcgataccgt
      541 gccgcggaag cgcagcgcag agttcagccg ccagtccagc ccccacttcc aggaccagac
      601 gttaccgctg ccggtgtact cggcagcgcg ggccgccgcc agcaggctca agtcctcggc
      661 ccacccctta tcgcgcaata acggtacctg ccactcggta aatgcttctt taacgacgta
      721 atggccatgg acgttcgcga ccgtagagtt ttggatcatc gagctacgtg tggcgatgtt
      781 cgaaggaaca gcgcgcagct cgggagccag cgcggccgac gccgggaaaa tgttggggtc
      841 gctggcgggg ttgctcggat cccaaacgat ctgctcgagc tcctctctgc gccaacctgc
      901 accgaacgcc gcaacgatgg ggccggccca cccatcccag agtcggccgc tggcggatag
      961 atcgatcact ttctgccgcg tctcggtgtt gtagaccttc ccgacaccgc tggtatagga
     1021 gatcgtctgg ccgctctcat actcgtccgg ctggaagaac aacggcgagg tgatctgctg
     1081 accgggggtg aagttggtga cgtaagagat ggctgcgtcg gaggcattgc cgcggccgaa
     1141 gagattcagc ggcacacaat cgccccactt gccggggttg atcgtggccg cgcggcacac
     1201 gatattgccg gacgggtcga ctacggcgtc cactgccgcc atcatgcgat ccagcagcac
     1261 gccttgttgg tagcctcggt tgtcggcctt gcccgactga acgtagccgt tcatctgcca
     1321 gtcgtcgccg aggtcccact tgaagccggc agtgccggag gtggtgtcgt tgtcttgctt
     1381 gaggcggctg gtgcgaccca aaccgtcgcg gtcgccgacg taattcattt ggaaggagtt
     1441 cagaccctcg tcgaccatga tctggcggac agccgccggc aagaaggcgt tctcccgaaa
     1501 gatcgtgatg cgcgtgtcga ggcccgcgat cgccgcgaag cggccgccat gatcgggctg
     1561 gtcggtcatg ctctggccgc ctagggcctg cacgtagaag ttcaagtcgg gcgttgcgtc
     1621 gatgctcagg tacgcgaacg cgctgtagcg ctcggcctcg ggcgacaggg ctagcaggtc
     1681 gcccgtcgtg tcgtctccgc tgccgccgtt ggtgatcgaa tgcgcatgtg tgggtgttcc
     1741 ttcgcccatg acgaatggcg cgagcgagcc gtcgggctgg aagaaccggc ggtacagagc
     1801 ggacgtggta ggcacgcccg agttgatgag gccgccgtta gtcgcgaccg ccgacacgac
     1861 gtccggccgg agcaggtagc gcggcgtcgc gttcgaggtg tcggggttgt tgatgtagct
     1921 gttgccctga taccagctgc gctcggcgta gctgtccacg ccgtccgcct ggtagtactc
     1981 gccactcaga agcagatgca ggcgctcccc gatcggcgct ccccacgcga tcgagtacag
     2041 cgagttgtcg tg
//
LOCUS       DASUGJ010000240         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1701289, whole genome shotgun sequence.
ACCESSION   DASUGJ010000240 DASUGJ010000000
VERSION     DASUGJ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701289"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VFV93_05280"
     CDS             complement(<1..1203)
                     /locus_tag="VFV93_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5164789.1"
                     /translation="MNLPATIDILICGGGTSGAALAGILARDTDANVVLLEAGPDYGP
                     LDSGRWPADLLDARRIPRTHGWGYAGSAHSAHVEETPFERAKVIGGCSSHNGCVALLG
                     HRRDYDAWAELGNDGWDWYSVAPAFERALRTLRVATPAESDVTPFHQSFIDGAVQFGI
                     PMSADMNDPDETAGVGASPVNVVDGTRWNTSLAYLDPVRRKGNLTVVGDALVDRIEVS
                     GGRAVAAHVVIDGTPQRIAAGRIVLAAGAYGSPAVLLRSGIGPTAETSAIGIAPVHDL
                     PGVGRALADHPAVNLRYRSSAQLDREMEEFARGHWSPDEQSLAKARSSICREAFDIHI
                     YAVAGWRTDDGDYVYGVAVSSVHPKSTGAITLRAADPTTPPRIDHNYLSDPDGEDLRV
                     LLDGVEMTR"
     gene            1349..2227
                     /locus_tag="VFV93_05285"
     CDS             1349..2227
                     /locus_tag="VFV93_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase subunit LigB"
                     /protein_id="HEX5164790.1"
                     /translation="MPLVVGAVAPHGFPIIPDISEDAEGALKTRESMLEMGRRFAEAQ
                     PEVVFIGGPHGIRVNGFISLADCARGAGTLHYQGRTVEMNVPIDLEFTDKVVALAKER
                     GVPVAQVGYGGSNRNQSILPMDWGVMTPLWFAGHDTNMAGKGHVLATLFDGVPESSGP
                     KAVIANPSRMLPRESNVELGKIVAEVAEADPRRIGFIASCDWAHRHDPDGPNGFHPDA
                     PKLDAEVVEAIKANDIMRLIDLDETYVSNAAIDGLWQILMLAGAMQVVPMDVTFQSYE
                     APTYYGMIVATYERAS"
     gene            complement(2288..2788)
                     /locus_tag="VFV93_05290"
     CDS             complement(2288..2788)
                     /locus_tag="VFV93_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5164791.1"
                     /translation="MTVQNQHNLTGATSILPTPRRYSLFGTILTILAERAGTAGHYDL
                     IEGETPAGLQTPPHRHTRYSELLYVIDGELTIWTADRNVILHTGESFSIDTGVPHVVA
                     ATGQRTARGLVIASPSGFAALIEQAGIPESNGGFREPTPADLERFNQLATEFGDEILG
                     QPGDLP"
     gene            complement(2920..>3260)
                     /locus_tag="VFV93_05295"
     CDS             complement(2920..>3260)
                     /locus_tag="VFV93_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164792.1"
                     /translation="GYDYIAATVRNMLPMILKTRIASADEIDIATLADRLRIATTAAD
                     SVVKMPDFVSAWSRKPFVTDSSKEETTCEKPASADEPATSAIATPSSLNGEDEPSAVH
                     AAATWTHSTG"
BASE COUNT          550 a          942 c         1182 g          586 t
ORIGIN      
        1 gcgagtcatc tcgacgccgt cgaggaggac gcgcagatcc tcgccgtcgg ggtcggagag
       61 gtagttgtga tcgatgcgcg gtggagtggt cgggtcggcc gcgcggagcg tgatcgcgcc
      121 ggttgacttc gggtggaccg aggagacggc gacgccgtag acataatctc catcgtccgt
      181 gcgccagccg gcgacggcgt agatgtggat gtcgaatgcc tcgcggcaga tgctgctgcg
      241 ggctttggcg agggattgct catcgggcga ccagtggccg cgagcgaact cctccatctc
      301 gcggtcgagt tgggccgagg agcggtagcg caggttgacg gccggatggt ccgccaacgc
      361 gcgaccaacg ccgggcaggt cgtggaccgg tgcgatgccg atggcagatg tttcagcagt
      421 cgggccgatg ccggagcgga ggaggacggc gggcgaaccg taggcaccgg cggcgaggac
      481 gatccgcccg gcggcgatgc gctgtggtgt gccgtcgatg acgacatgtg cagcgacggc
      541 gcggccgccg ctgacctcga ttcgatccac gagcgcatcg ccgacgacgg tcagattgcc
      601 cttgcgacga acggggtcga ggtaggcgag ggaggtgttc cagcgggtgc cgtcgacgac
      661 gttgaccggc gatgcgccga caccggcggt ttcgtcggga tcgttcatgt ccgctgacat
      721 cgggatgccg aattggacag cgccgtcgat gaacgactgg tggaacgggg tgacatccga
      781 ctcagcgggc gtcgcgactc gcaacgtccg gagcgcgcgt tcgaaggccg gagcgaccga
      841 gtaccagtcc cagccgtcat tgcccaactc ggcccaggcg tcgtagtcgc gacggtggcc
      901 gagcagggcg acacagccgt tgtgcgacga gcagccgccg atgacttttg cccgctcgaa
      961 tggtgtctcc tcgacgtggg cgctgtgcgc cgatccggcg tagccccagc cgtgggtccg
     1021 cgggatgcgg cgtgcgtcca gcagatcggc cggccagcgt ccgctgtcaa gtggtccgta
     1081 gtcggggccg gcttcgagca gaacgacatt ggcgtcggtg tctcgggcca ggatgccggc
     1141 cagggcagcg ccgctcgttc cgccgccgca aatcaggata tcgattgtcg ccgggagatt
     1201 catctgattg agtcctgtct ttcgcgcagc gatgtcacat gccaatcgtc cgtgaactca
     1261 ggcgatggtg caagcccatc gcgcgtgact gtcgtaccat ggaaccgaca tcgaggttgg
     1321 gcatggatgg aatcgcaggg agggcagaat gccgcttgtc gttggcgcgg tcgcgccgca
     1381 tggattcccg atcattccgg atatcagcga ggacgccgaa ggggcgctga agacgcgcga
     1441 gtcgatgctg gagatgggcc ggcgcttcgc ggaggcgcaa ccggaggtcg tcttcatcgg
     1501 cgggccgcat gggatcaggg tcaacggatt catctcgctg gcggattgtg ctcggggcgc
     1561 gggaacgttg cactatcagg ggcgaacggt cgagatgaac gtgccgatcg accttgagtt
     1621 caccgacaag gtcgtcgcgc tggcgaagga acgtggcgtg ccggtcgcgc aggtcgggta
     1681 cggcggctcg aaccgcaacc agtcgatcct gccgatggac tggggcgtga tgacgccgct
     1741 ctggttcgcc ggtcacgaca cgaacatggc cggcaagggg catgtcctgg cgacgttgtt
     1801 cgacggcgtg ccggaatcga gcgggccgaa ggcagtcatc gccaacccgt cgcggatgct
     1861 gccgcgcgag tcgaacgtcg agcttgggaa gatcgtcgcc gaggtcgccg aagccgaccc
     1921 gcgccggatc ggcttcatcg cgtcgtgtga ctgggcgcac aggcacgatc ccgacgggcc
     1981 gaacggcttc caccccgacg cgccgaagct ggacgccgag gtggtcgagg cgatcaaggc
     2041 caacgacatc atgcgcttga tcgatctgga cgagacgtac gtgagcaacg cggccatcga
     2101 cggtctctgg cagatcctga tgctggccgg agcgatgcag gtcgtgccaa tggacgtgac
     2161 gttccaatcg tacgaagcgc cgacctacta cgggatgatc gtcgcgacct acgaacgggc
     2221 cagctagacg acgaaatgag cggagtaatc ggttactccg ctcgtcatct tacttcacgc
     2281 gcttcgatca gggtagatcg cccggctggc cgaggatctc gtcgccgaat tcggtggcaa
     2341 gctggttgaa acgttcaaga tcggctggtg tcggttcccg gaagccgccg ttgctctccg
     2401 ggataccggc ctgttcgatc aaagcggcga agccgcttgg ggaggcgatg acgagacccc
     2461 gggcggtgcg ctggccggtt gcggcaacga cgtgcgggac gccggtgtcg atgctgaacg
     2521 actcgccggt gtgcaggatg acgttccgat cggcggtcca gatcgtcagt tcgccgtcga
     2581 tgacgtagag cagctcgctg tagcgggtgt gccggtgtgg cggcgtttga agaccggctg
     2641 gcgtttcgcc ttcgatcaga tcgtagtgtc ccgctgttcc cgcccgttcg gccagaatcg
     2701 tgagtatcgt gccaaagagc gagtaccggc gcggcgtcgg aagtatgctg gttgctccgg
     2761 tcaggttgtg ctggttttgg actgtcaaga tgcaggtcct tcctacagga ggatcggatg
     2821 ctttcgaccg gttctgcctt ggcaatcaga acgcgcgaac cgggacgacc tcgcgagtac
     2881 gcgagcagcg gttgaagagc cggttgcgca ggtcaggggc tagccagtcg agtgtgtcca
     2941 ggtggccgca gcgtggacag cggagggctc gtcctccccg ttcaaagagg acggcgtcgc
     3001 gatcgctgat gtcgccggtt cgtccgcaga agcaggtttc tcgcatgtcg tttcctcctt
     3061 gctggaatca gtcacgaatg gcttgcgcga ccaggcgctg acgaaatcgg gcatcttgac
     3121 gacgctgtcg gcggcggtcg ttgcgatcct cagccggtca gcgagcgtcg caatgtcgat
     3181 ctcgtctgcg ctggcgatgc gggtcttgag gatcattggc agcatgtttc gaacggtcgc
     3241 ggcgatgtag tcgtacccgg
//
LOCUS       DASUGJ010000241         3616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_312626, whole genome shotgun sequence.
ACCESSION   DASUGJ010000241 DASUGJ010000000
VERSION     DASUGJ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312626"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1705)
                     /locus_tag="VFV93_05300"
     CDS             complement(116..1705)
                     /locus_tag="VFV93_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX5164793.1"
                     /translation="MNLIKSLVLGSAAGLVAMSGAQAADLPVKAKAVEYVRICSLYGA
                     GFFYIPGTDTCIKLGGYLRVDTTFNGGIYGQPAYSGDNGQRNRYRDYFASRSRMAVTI
                     DTRTATEYGVVRTFGQGDFQFNNFGSSNPNTLTSFPGGASNALNVPGGGYVAVEMVFI
                     QFAGFTFGKSASAFATPWNGYPGNNNSFLMGGPDYVTGVNNIQYTAQFGNGVSATIGL
                     DDPTVFGRTSVLNLGVAATTAGLALNASPTAAGANAYGGTHVPDIVGNVRVDQAWGLF
                     QIAGALHNVNASYNILGAGGVPTALSEISGHPEDKWGGAVTAALQIKNIPTGPGDDIK
                     FDATYAKGMTKAVISTSGASPSFAMFGGTNRLGAYQSIGFGQTSDAVYLPGVFTGGLT
                     GDLKLTDAWGIRGAYNHNWDPYWSTSLWGSYAQVRYGGGALDILSAKGQFCASFNSGK
                     VVSADYSCNPDFNIAQVGVVTRWTPVKNLTFSAEVGAFFLDQKFSGAIAATAPAPKPT
                     TVYEFKDQSTVYLNVRAQRNF"
     gene            2091..2630
                     /locus_tag="VFV93_05305"
     CDS             2091..2630
                     /locus_tag="VFV93_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX5164794.1"
                     /translation="MGQEPRKGSDLASRSKRGGSNEPGGKNHDIARQFAWEIAAINVY
                     LQEIRYFWAKSLGVSGPQWMILMALADLDQGEGVSVKVVSKMLHVDPSFVTTQSKMLE
                     KKGFVRRKTSGDDARVVQMSLTDKTYKFIAGLASQQEELNHFIFGEFSERELSEFAGK
                     LAALKSRLEKARMKVEMGI"
     gene            complement(2643..>3616)
                     /locus_tag="VFV93_05310"
     CDS             complement(2643..>3616)
                     /locus_tag="VFV93_05310"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5164795.1"
                     /translation="IDPNDVVGSWYREWKGIAGVSRERGDAAFARGHVLTAQNNWLRA
                     INYYQASTFAFDAADEKRQDAVEAMRACARRYIAHLAPAGEVVEIPWLEGYALEGYFL
                     PAANASDRTPVVVCMGDPGHRKEEYLFKVARHARERGVSLLAADLLGPGVGSEFDAVI
                     GRPDLETSVHSVMDYLTMRDDVDEQRVAIVGDGAGSSFVARGVALDHRFAAAVCDGGI
                     WDMHEHALLMDRLSPDGAVDGGLERSWPAHRFHCPVLVTVGGQGWLESGTVADLFEQL
                     KARRSAISLKIFESSETAAAQGHSDNPTLANEFIFDWVADRLESIPA"
BASE COUNT          772 a         1080 c         1115 g          649 t
ORIGIN      
        1 agtctcctgt gcacgatgga ttccggggcg gcgagagaga aggcgaacat caaaaaatag
       61 gcccccagcg atttccgctg agggcccgtg ttgcagagtt gccagatgtt cgggatcaga
      121 agttacgctg agcgcgaacg ttcaggtaga cagtgctctg gtccttgaac tcgtacacgg
      181 tcgtcggctt gggagccgga gccgtagcag cgatagcgcc cgagaacttc tggtcgagga
      241 agaacgcgcc gacttcagcc gagaacgtca ggttcttgac gggagtccag cgggtgacga
      301 caccgacctg ggcaatgttg aagtcggggt tgcagctata gtctgcagac acaaccttgc
      361 cagagttgaa gctcgcacag aactgaccct tggcggacag gatgtcgagg gcgccaccac
      421 cgtagcgaac ctgggcatag ctgccccaga ggctggtcga ccagtaggga tcccagttgt
      481 ggttgtacgc accacggata ccccacgcgt ccgtcagctt caggtcgccg gtaagaccgc
      541 cggtaaacac cccgggcagg taaaccgcat cagacgtttg gccaaagcca atgctctgat
      601 aggcacccag gcggttggtt ccaccgaaca tcgcgaagct cggcgaagca ccggaggtcg
      661 agatcacggc cttcgtcata cccttggcgt aggtggcgtc gaacttgatg tcgtcgcccg
      721 ggccggtcgg gatgttcttg atctgcaacg cagccgtcac agctccgccc cacttgtctt
      781 cggggtgacc ggagatttcc gacagagcgg tcggaacgcc accggcgccg aggatgttgt
      841 aggaagcgtt gacgttatgc aatgcgcccg caatctggaa cagaccccag gcctgatcga
      901 cacggacgtt accgacgatg tcgggcacat gcgtgccgcc ataggcgttg gcaccagcgg
      961 ccgtcggcga ggcgttaagc gcaaggcctg cggtcgtggc ggctacaccc aggttgagca
     1021 cggaggtgcg gccgaacacg gtcggatcat cgaggccgat ggtggccgac acgccgttgc
     1081 cgaactgcgc ggtgtactgg atgttgttga cgccggtgac gtagtcggga ccgcccatca
     1141 agaacgagtt gttgttgccc ggatagccgt tccacggcgt cgcgaaggcc gaagccgact
     1201 taccgaaggt gaaaccggcg aactggatga acaccatttc aaccgcgaca tagccgccgc
     1261 cggggacgtt gagcgcgttg gaagcgccgc ccgggaagga cgtgagcgtg ttcgggttcg
     1321 aactgccgaa gttgttgaac tggaagtcgc cctgaccgaa ggtacggaca acgccgtatt
     1381 ccgtcgcggt gcgggtatcg atcgtcaccg ccatacggga acgggaagcg aagtagtcgc
     1441 gatagcgatt gcgctgaccg ttgtcgcccg aatacgccgg ctgaccgtaa ataccaccgt
     1501 tgaacgtcgt atcaacgcgc aggtaaccac ccagcttgat gcaggtgtcg gtgcccggga
     1561 tatagaagaa acccgcacca tacagggagc agatcctcac atactcgacc gctttggcct
     1621 tgacgggaag atcggcagcc tgtgccccgc tcatggcgac gagacccgcc gctgagccga
     1681 gaacaaggct cttgatcaag ttcatgttaa acctccaagt tgctctggca agacgaggac
     1741 ggaccttgtg gctgttcccc cagagaaagg tccgtcctca tcccttgaaa accgctcagt
     1801 cgtttcgcgt cttcggacac acccgcatga acgcgaggga cttgagcgaa cttctaaaac
     1861 ggaacgatcg aggacccccc tcgccgtcac cagaactatg cctgtgcgct ttaattaatc
     1921 aaaatacttt atgaactcag gtaacttgtt cgggagcgac tgtgtcgccg cggcaacacc
     1981 ggcgacgacg aactttttgc gtcgatagtt cgatcgtgaa ctcccaattg gatgaatttg
     2041 ttattaaatc agctaagttg ggcctcgcag actaagctgg aaaaagagtc gtgggacaag
     2101 aaccgcgcaa gggatcggac ctggcgagcc gcagcaaacg cggcggctcg aatgagccag
     2161 gcgggaagaa tcacgacatc gcccggcagt tcgcctggga gatcgccgcc ataaacgtgt
     2221 atctgcagga gattcgctat ttctgggcga aatcgctcgg cgtcagcgga ccgcaatgga
     2281 tgattctgat ggcgctcgcc gatctggatc agggagaagg cgtgtccgtg aaggtcgtgt
     2341 caaagatgct ccatgtcgac ccgtccttcg tgaccaccca gtcgaagatg ctggagaaga
     2401 agggcttcgt gcgccggaag acgtcgggcg acgacgcccg cgtcgtgcag atgtccctca
     2461 cggacaagac atataagttc atcgccggcc tcgcctccca gcaggaggag ctcaaccatt
     2521 tcatcttcgg cgaattcagc gaacgcgaac tcagcgagtt cgccggcaag ctcgcagcgc
     2581 tgaagagccg gctcgagaag gcccgcatga aggtcgagat gggtatctga tcgcttgcgg
     2641 cctcaggccg ggatcgattc gaggcggtcg gcgacccaat cgaagatgaa ttcgttggcc
     2701 agcgtcgggt tgtcgctatg gccctgggct gcggccgttt ccgagctttc gaaaatcttc
     2761 agtgaaattg cggaacgacg ggccttaagc tgttcgaaca gatcggccac cgtgccgctt
     2821 tcgagccagc cctgcccgcc cacggtgacc aggacgggac aatgaaaccg gtgcgcgggc
     2881 cagcttcgtt caagtcctcc atccaccgca ccatccggcg agaggcgatc cataaggagg
     2941 gcatgctcat gcatgtccca gatcccgccg tcacagaccg cggcggcgaa acgatggtcg
     3001 agcgcgacgc cacgcgccac aaaggacgac cctgcgccat cgccgacgat ggctactcgt
     3061 tgctcgtcga catcgtcccg catcgtcagg taatccatca cggagtggac cgacgtttcc
     3121 agatcgggac ggccgatcac cgcgtcgaat tcactgccga cgccgggacc aagaagatcc
     3181 gcggccagca acgacacacc gcgctcgcga gcgtgacgcg cgaccttgaa gagatactct
     3241 tccttgcgat gtccgggatc gcccatgcaa acgacgacgg gcgttcggtc cgatgcgttc
     3301 gccgcgggca ggaaatagcc ctccagcgcg tatccttcca gccagggaat ctcgaccacc
     3361 tcaccggccg gcgccagatg cgcgatgtag cggcgcgcgc aggcgcgcat ggcctcgacc
     3421 gcatcctgcc gcttctcgtc cgcagcgtcg aatgcaaatg tcgatgcctg ataataattg
     3481 atggcgcgaa gccagttatt ttgcgcagtc agcacatgcc ctcgtgcgaa ggcggcgtcg
     3541 ccgcgctcgc ggctgacgcc ggcgatccct ttccactccc gataccagga gccgacaacg
     3601 tcgttcgggt cgatcc
//
LOCUS       DASUGJ010000242         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1008109, whole genome shotgun sequence.
ACCESSION   DASUGJ010000242 DASUGJ010000000
VERSION     DASUGJ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008109"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..511
                     /locus_tag="VFV93_05315"
     CDS             350..511
                     /locus_tag="VFV93_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164796.1"
                     /translation="MSAEHVNQEPLDPNDPNYWIYLMHDIEIIAEPIDQPTNAGLAFY
                     GKRPTRKAS"
     gene            complement(515..1456)
                     /locus_tag="VFV93_05320"
                     /pseudo
     CDS             complement(515..1456)
                     /locus_tag="VFV93_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
     gene            complement(1589..3007)
                     /locus_tag="VFV93_05325"
     CDS             complement(1589..3007)
                     /locus_tag="VFV93_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5164797.1"
                     /translation="MVSSQTTTSATPAIDTAAIDAFAATLRGQLIRPGDADYEIARRV
                     HNGMIDKHPALIARCVDVADVIAGVNFAREHGLLLAIRGGGHNGPGFGTCDGGLVLDL
                     SPLKGIRVDPVARTARVEGGCTLGDVDHATHAFGLATPLGIASTTGVAGLTLGGGMGH
                     LARHYGLAIDNLLEVDIVLADGSFVTASEQQHPDLFWAIRGGGGNFGVVTSFLFRLHP
                     VSTVYAGPMLWHMDQAEDVLRWYNEFQPNSPDELNGSLMIISVPPGEPFPEALWNETM
                     CAIVWCYSGSLDQAEDLFLPVRTRFGTPALDWVGPMPYPVLQTMFDAVFPPGIQTYWK
                     ADFVNELSAESIAIRLEQARRKPVGPSLIGFHPIDGATSRVSNDATAWSYRDARWAEV
                     MAGVTHDPAENEAIIGWARETWDALHPFSAGGAYVNFMMEEGVDRIQATYRGSYDRLA
                     RVKAIYDPGNLFRVNQNIAPKS"
BASE COUNT          581 a         1142 c         1052 g          564 t
ORIGIN      
        1 tccattgcgc gcatatcgtc gccgattcag gcgcgtccgg caacttgcga tccatccgcg
       61 cccgattagc agcgaagtac cgtgccggca tccctgatcg gcaggaccaa ccacgcggca
      121 ccaatcggct accggcagtc aggacttccg ggccgtgtcc ggcaggcatc gttttgcaac
      181 aatgacatca atgaccagtt gaaacgagga tcggagcgaa cggcaattgt cctaccgcgc
      241 tcaatgccct gatggcacct gtgtcccgtc ccgctcgggc ggcttctgcc tgctctgcgg
      301 ccgggctctc cgctccgcgc gcacgacgga cgacgaattg caggcagcct tgagcgcgga
      361 gcacgttaac caagagccgc tcgatcccaa cgaccccaac tactggatct acctcatgca
      421 cgacatcgaa atcatcgccg aaccgatcga ccaacccacc aacgccggcc tcgccttcta
      481 cggcaagcga ccgacccgca aggcaagctg accgctacgg ctcgtcccgt ttaaggaatc
      541 cgctcataca gtggatgccg ccgccggctt tgatcagttc ggagacatcg acttcgacgg
      601 tttcgactcc ggcttcgtgg tagcgctcgc gcatgcgctc gcccccggtt ggcatcagga
      661 tcttgccggg agccagcgcg acgaagttga gcgggagctg ctcgtgcgcc tcgacgtcgt
      721 cgatgacttc gaggatccgg aagccgcgct gctggagcgt tcagacgatc gggaacggcg
      781 tccggcgcgg ccagacgacg gccaggtcgc ggtcggcgaa gttgagcatg ccgtcgaggt
      841 gcatcgcgcc ccatggcagg tggacgcgaa cgacctcgcg gacgccaatc tcacgcagca
      901 tccgctcgac ctggtccgca ccctcggtgt tggtgcgcag tccctcggcc aggaagcaca
      961 ggtccttgtc gacccacatc acgtcggcgc cctcgaaggt gccgttgccg tggaccgaca
     1021 tcaggatcgg gacgcccagc cgccccagcg cctcggcgac gaaacgttcc tcgccggcgc
     1081 ggacggtcga ggccggccgg gtgatgatcg cgccttccgg tgtcatcagc atcaggtcgc
     1141 ggatgaagaa cgagttgggc ttgtccagcc ggccgttctc gccgtagtgg acgctgacgc
     1201 cgtgtgcctc gtaggcgtcg gccatcgcgt cgtgctcgcg gcgggcgacc tccggcagca
     1261 gatcggagcg catctggacg gcgtcgaagt cctcgatggc gtccagctcc gggccgggcc
     1321 gccgcagcag gacggcatgc agccgcccgc actccgaact ggagccccag ttaccccacc
     1381 actccggcat gtcctcgcgc atcgacgccg tgcgcggcga ccacttcgcg cccccatacg
     1441 cagccgtcag gctcatcgca acaccctccc cacttcgtca acccacgttg cagtgggcat
     1501 tgtaggcgag gtgaggggcc acgggcgatc agaggggctg gcctgacgcg gcggcacgag
     1561 tacgcgatcg cgccgccgcg actggtacct atgacttcgg cgcaatgttc tggttgacgc
     1621 ggaagaggtt gccgggatcg tagatcgcct tgacgcgcgc cagccggtcg tagctgccgc
     1681 ggtaggtcgc ctggatgcgg tcgacgccct cttccatcat gaagttgacg tacgcgccgc
     1741 cggccgagaa aggatgcagc gcgtcccagg tctcgcgcgc ccagccgatg atcgcctcgt
     1801 tctcagccgg atcgtgcgtc actccggcca tcacctcagc ccacctggcg tcgcggtagc
     1861 tccaggctgt cgcatcgttg ctgaccctgc ttgtcgctcc gtcgatcggg tggaagccta
     1921 tcagcgacgg tccaaccggc ttgcgcctgg cttgctcgag acggatcgcg atcgactcgg
     1981 cggacagttc attgacgaag tccgccttcc agtacgtctg gatgccgggc gggaagactg
     2041 cgtcgaacat cgtctgcagg acaggatacg gcatcgggcc aacccagtcg agcgcgggcg
     2101 tgccgaagcg ggtacgaacc ggtaggaaca gatcctcagc ctgatcgagc gaacccgagt
     2161 agcaccagac gatcgcgcac atcgtctcgt tccagagcgc ctccgggaag ggttcgccgg
     2221 gtggcacgga gatgatcatc aacgagccgt tgagttcgtc cggcgaattc ggctggaact
     2281 cgttgtacca gcgcagcacg tcttcggcct ggtccatgtg ccagagcatc gggccggcat
     2341 agaccgtgct gaccggatga agccggaaca ggaacgaggt aaccaccccg aagttgccgc
     2401 cgccgccacg gatcgcccag aacaggtcgg gatgttgctg ctcgctggcg gtcacgaagc
     2461 tgccgtcggc cagcacgatg tcgacttcca gcaggttgtc gatcgccagc ccatagtgac
     2521 gagcgagatg acccatcccg ccgcccagcg tcagccccgc gacaccggtc gtcgaggcga
     2581 tcccgagcgg ggttgccagt ccgaacgcgt gggtcgcgtg gtccacgtcg ccgagcgtgc
     2641 agcctccctc gacgcgggcc gtccgggcga ccggatcgac ccggatcccc ttcagcggtg
     2701 agaggtcgag caccagcccg ccgtcgcagg tgccgaagcc gggaccgttg tgtccaccgc
     2761 cacggatcgc cagcagcaga ccatgctcgc gcgcgaagtt cactccggcg atgacatcag
     2821 cgacatccac acagcgagcg atcagcgccg gatgcttgtc gatcatccca ttgtggaccc
     2881 ggcgggcgat ctcgtagtcc gcatcgcccg gccggatgag ctgtccccgc aacgtcgcgg
     2941 cgaaggcgtc gatcgcagcc gtgtcgatcg cgggcgtcgc gctggttgtc gtctgactgg
     3001 acaccatctg cacaccacct ctctccggcg ccgaaagcgc cagaattcgg gtcgcgcgaa
     3061 cccagcctcc catcgggccg gatccgcgca atcactcctt gcatcacgtg tcttcacagt
     3121 actgccgaac cgaaggcggc acatcatcag gtctgatgat ctttttgcgg tctggtcgat
     3181 gagttcgcgt gcgaaggctc acccctcgtt cagcaggccc atttcaacag cccagatcgc
     3241 cacttgcgtg cgggagttgt aaccgagctt gctgaggacg tggctgacat gggtcgcgac
     3301 cgtcggctcg ctgacgatca gccgttcggc gatcatcct
//
LOCUS       DASUGJ010000243         5105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_173678, whole genome shotgun sequence.
ACCESSION   DASUGJ010000243 DASUGJ010000000
VERSION     DASUGJ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173678"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /gene="hemB"
                     /locus_tag="VFV93_05330"
     CDS             complement(<1..964)
                     /gene="hemB"
                     /locus_tag="VFV93_05330"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5164798.1"
                     /translation="MTGSVDTQWPPFRRFRRLRQSDGLRRFARETVLLPSDFVYPLFV
                     TYGHGVRREVPSMPDVYQISVDQLAREADDLRSLGIGSVLLFGIPEHKDPLASDAYAD
                     NGIIQQAIRELKRVDPGLVVIGDVCCCEYTDHGHCGILNGEIVDNDQTLTLLARTALA
                     QAEAGVDIVAPSDMMDGRVLVLRQALDGAGYSNLPILSYAAKYASGFYGPFRDAAGST
                     PSFGDRRSHQMDPGNAREALAEIETDLAEGADAVIVKPALPYLDVIAAARQRFDVPIA
                     AYNVSGEYAMVRAAGLAGWIDERRVTMETLTSIRRAGANQIITYH"
     gene            complement(961..1764)
                     /locus_tag="VFV93_05335"
     CDS             complement(961..1764)
                     /locus_tag="VFV93_05335"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX5164799.1"
                     /translation="MTLATPAGRPLTGQRVLVTRARPQAADLTARLKALGASVVALPA
                     IEIVPVDPAPLDAALRQISSYDWLVLTSSNGVAAVIQRLDALGLRPSVLNETQVAAIG
                     KATAASLRRYYLSVDLVPDHFVGESVVAALVARGVAGRRILLPQAEIARETVAEGLRQ
                     AGAEVDAIVAYRTALPDADAVEDVRQMLAGVDIATFASPSAVRNTLTLAGGTLTGPRV
                     VCIGPVTAEAACGAGLRVDAVADEYSIDGLIAAVVRLAANDLKIEGGSR"
     gene            complement(1761..2699)
                     /gene="hemC"
                     /locus_tag="VFV93_05340"
     CDS             complement(1761..2699)
                     /gene="hemC"
                     /locus_tag="VFV93_05340"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX5164800.1"
                     /translation="MSSTIDPAPPATLRIGTRGSRLALLQTERVVAFLRQQYPDLVVE
                     TVVISTQGDRDKQTPLSAIGGQAVFAKDLQQSVLSGEVDCGVHSAKDLTSTMPEGLTL
                     AAILDRADPRDVLISRHPGGLAGLPAGARVGTSSRRRMAQLRLARPDLKPIELRGNID
                     TRLVKVLDTPDGPYDAAILAAAGVLRMGWEERISEYLDVETFTPAPGQGALGVDCRES
                     DRATRRLLAAVADPDATAEVEAERAFLRTVGGGCTSPLAAHARVEGEILRMWCMYADE
                     SMDRIATAEDEADVEDGAALGDRLARMLQDRVEAEQ"
     gene            3074..3388
                     /gene="rplU"
                     /locus_tag="VFV93_05345"
     CDS             3074..3388
                     /gene="rplU"
                     /locus_tag="VFV93_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX5164801.1"
                     /translation="MYAVIETGGKQYRVAAGDTIDVERLPDDAGAQVEIERVLMVAGD
                     EGITIGAPVVAGAAVVATVTEHLRGPKIVVFKFKPKKRYRRKTGHRQELTRLKIDSIR
                     TA"
     gene            3425..3730
                     /gene="rpmA"
                     /locus_tag="VFV93_05350"
     CDS             3425..3730
                     /gene="rpmA"
                     /locus_tag="VFV93_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX5164802.1"
                     /translation="MAHKKGMGSSRNGRDSKAKRLGVKLYDGNAVRGGGIIVRQKGTK
                     FYPGNNVGMGRDYTLFSLVDGLVKFEQVRRDRKQVSVYPFEVVDAAAAAEEQAVVAD"
     gene            3795..3980
                     /gene="rpmE"
                     /locus_tag="VFV93_05355"
                     /pseudo
     CDS             3795..3980
                     /gene="rpmE"
                     /locus_tag="VFV93_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            4380..4586
                     /locus_tag="VFV93_05360"
     CDS             4380..4586
                     /locus_tag="VFV93_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164803.1"
                     /translation="MGAASLSPRTTEVTVDDLLAVGFTDDQIERLQALKNAYPFIEYV
                     DTAKQWRRLEFLKWRYLQGDLQRA"
     gene            4738..>5105
                     /locus_tag="VFV93_05365"
     CDS             4738..>5105
                     /locus_tag="VFV93_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5164804.1"
                     /translation="MAAQERTPEVVIVGGGIIGCSLAWELAKAGVTSRVIERREIARE
                     ASWASAGIISAPGPRHGARAGLALDAFRRYPELIAEIEDITGRSVGYVRSGELLLADN
                     ETLDAARETFSWQQVNGVAV"
BASE COUNT          939 a         1665 c         1687 g          814 t
ORIGIN      
        1 cgtggtaggt gatgatctgg ttcgcgccgg cccgtcggat cgaggtcagc gtctccatcg
       61 tcacgcgccg ctcgtcgatc cagccggcca gaccggctgc ccggaccatc gcgtactcgc
      121 cactgacgtt gtaggcggca atcggtacgt cgaagcgctg tcgggcggcc gcgatgacat
      181 ccaggtatgg cagggcaggc ttgacgatga cggcatcggc cccttcggcc agatcggtct
      241 cgatctcggc cagcgcctca cgggcgttgc cggggtccat ctggtggctg cgccggtcgc
      301 cgaagctcgg cgtcgatccg gcagcgtcgc ggaacggccc gtagaagcct gaggcgtact
      361 tggcggcgta ggacagaatc ggcaggttgg agtagccggc tccgtcgagc gcctgccgta
      421 ggaccagcac gcggccatcc atcatgtcgg acggcgcgac gatatcgacg ccggcctcgg
      481 cctgagccag tgccgtccgc gccagcagcg tcagcgtctg atcgttgtcg acaatctcgc
      541 cgttgaggat gccgcagtgg ccgtggtcgg tgtactcgca gcagcagaca tcgccgatca
      601 cgaccaggcc gggatcaacc cgcttcagtt cccgaatcgc ctgctggatg atgccgttgt
      661 cggcataggc gtcggacgcc agcggatcct tgtgctcggg gatgccaaac agcaggaccg
      721 aaccgatccc cagcgaacgc aggtcgtcgg cctcccgcgc gagctgatcg accgaaatct
      781 ggtagacatc cggcatcgac ggcacctcgc gacggacgcc gtgcccataa gtaacgaata
      841 gcgggtagac gaagtcggac ggcagcagga ccgtctcccg cgcgaagcgg cgcaacccgt
      901 cgctctgccg caggcgacgg aaccggcgga acggaggcca ctgtgtgtca acggagccgg
      961 tcatcgggaa cctccttcaa tcttcagatc gttcgctgcg agccgcacaa cggcggcaat
     1021 cagtccatcg atcgagtatt cgtccgcaac agcatcgacg cgcaaacctg cgccgcaggc
     1081 cgcctcggcg gtgaccggcc cgatacagac gacgcgcggc ccggtcaacg ttccgcctgc
     1141 cagcgtcagc gtattcctga ccgccgaggg cgacgcaaag gtcgcgatat cgacgcccgc
     1201 aagcatctgc cgcacgtcct cgacggcatc agcatccggg agcgccgtcc gataggcgac
     1261 gatggcgtcg acctccgcgc ctgcctgtcg aagtccctcg gcgacagtct cgcgcgcgat
     1321 ctccgcctgc ggcagtagaa tgcgccggcc ggcaacgccg cgtgcaacca gcgcagcgac
     1381 gactgactca ccgacgaagt ggtccggaac gaggtcgaca ctgaggtagt aacggcgcaa
     1441 gctggccgcc gttgccttgc cgatcgccgc gacctgcgtc tcgttcaaca ccgaaggacg
     1501 cagtccaagc gcgtcgagtc gctggatgac ggccgcgacg ccgttcgagc tggtcaggac
     1561 gagccagtcg tagcttgaga tctgccgcag tgcagcatcc agcggagcgg gatcgaccgg
     1621 aacgatctcg atggcgggaa gcgcgacgac cgacgcgccg agcgccttca gccgcgccgt
     1681 caggtcggcc gcctgcgggc gggcgcgcgt caccagcacc cgctgccccg tcagcggccg
     1741 gccggccggc gtcgccaacg tcattgctcg gcctcgaccc gatcctgaag catccgggcc
     1801 agccggtcgc ccagtgcggc gccatcctca acgtcggcct cgtcctcggc cgtcgcgatc
     1861 ctgtccatcg actcgtcggc gtacatgcac cacatccgca ggatctcgcc ttccacccgc
     1921 gcgtgagcgg ccagcggcga cgtgcagcca ccgccgacgg tgcgcagaaa ggcccgctcg
     1981 gcctcaacct cggccgttgc gtcgggatcg gccactgccg ccagcaaccg gcgggtcgcc
     2041 cggtcggact cgcggcaatc gacgccgagc gcgccctggc ccggcgcggg cgtaaacgtc
     2101 tcgacatcga gatactcgct gatgcgctct tcccagccca tccggaggac accggccgcc
     2161 gccagaatcg cggcgtcgta cgggccgtcc ggcgtgtcga gcaccttcac gaggcgcgta
     2221 tcgatgttgc cgcgtagctc gatcggtttc aggtcggggc gggccagccg gagttgcgcc
     2281 atccgtcgcc ggctgctggt gccaacccgc gctccggccg gcaaaccggc caacccgccc
     2341 ggatgacggg agatcagcac gtctctgggg tcggcccggt cgaggatcgc cgccagcgtc
     2401 agcccttccg gcatcgtcga ggtcaaatcc ttggcgctgt ggacgccgca atccacttcg
     2461 ccgctcagga ccgactgctg gagatccttg gcgaagacgg cctggccgcc gatcgccgag
     2521 aggggagtct gcttgtcgcg gtcgccctgg gtggagatga cgaccgtctc tacgaccaga
     2581 tcgggatact gttggcgcag gaacgcgacg acgcgctcgg tctggagcaa cgccaaccgg
     2641 ctgccgcgcg tgccgatccg caacgtcgcc ggcggcgcgg gatcgatcgt cgaactcatg
     2701 actgtgctcc gtctccggtc ggttgcggcc gttcgtgtgc gctcagagga gaatacggaa
     2761 cggtcagtcg cctggaatcg cccgaaaatc tgggcaaata tgactacata aaaagtgtag
     2821 gagtcgcgtc gatccattgt caaggagttt gtgaagtcgc tcacgaacag tatgatggaa
     2881 tgcatcggcg cacttccggg acgatcggac gatgcgacag gcccgaattg acgatcacgt
     2941 gggcatacga tacacttacg ggccggcagg tcgcgtctcg gcgctgacct gttttcatgt
     3001 tgtgcaatgc atcaggatca accggcacgc tgatgatgcc ggacatgcca gcgccaaacg
     3061 gggaggggac acggtgtacg cggtcattga aacaggcggt aagcagtacc gcgttgctgc
     3121 cggagacacg atcgacgtcg agcgcctgcc ggacgatgcc ggggcgcagg tcgagatcga
     3181 gcgcgtcctg atggtcgctg gcgacgaggg catcacgatc ggcgcgccgg tcgtggccgg
     3241 tgcggcggtc gtcgcgaccg tcaccgagca cctgcgcggc ccgaagatcg tcgtcttcaa
     3301 gttcaagccg aagaagcgtt accgccgcaa gacgggccat cgccaggagc tgacccggct
     3361 gaagatcgat tcgatccgca cggcctgagg gcgcgcgacc acgtctgaga cgggagttta
     3421 agagatggca cacaaaaagg gcatgggtag ttcccgcaac ggccgcgaca gcaaggccaa
     3481 gcggctgggc gtcaagctct acgacggcaa tgcggttcgc ggcggcggca tcatcgtccg
     3541 ccagaagggg accaagttct acccaggcaa caacgtggga atgggacgcg actatacgtt
     3601 gttctcatta gtagacggcc ttgtcaagtt cgagcaggtc aggcgcgatc gcaagcaggt
     3661 cagcgtctat ccgtttgagg tcgtcgacgc ggcggctgcc gccgaggagc aggccgtcgt
     3721 cgcggactag ttccatcgca ttggctgcac ggggcacagc gcccgcgcgc agcaggatcg
     3781 ggagattcat cactgtgaag cagggcattc acccaaacta tcaggccgca acggtcacct
     3841 gtgcctgcgg caacacctgg gaaaccaggt cgaccagacc gaacctgcgc gtcgatctgt
     3901 gcaacaagtg ccatcccttt tacactggcg agcagcgcat cgttgacacg gccggacagg
     3961 tcgagcgttt catgcgccgc ctcgaaggag cgcagggcgc gccgcgccgc aagaaggccg
     4021 agcgtcgcca ggcgaagatc gatcagcgca agaggatcga aggccgcgac cagcagttgc
     4081 tggtagccgg catcgaggac gaggaaccaa ccagctagct cgaccggagc tcatttctca
     4141 gcacatccat cccgccccgg tccataactg gacgtcgggg gcgggattcc tatatctgga
     4201 ggattccacc acggcccgct accgtggcaa ggggtttgca taggagacga cgagacgaat
     4261 tgagggcgcg gaaccatccg cggggccact ctcgtcgtag aacacagtga gtggtgtccg
     4321 catccatgcg gactgttgac cggcagagac gctggtcctg gaaagaagga gcgaccgcaa
     4381 tgggtgcagc atcgctctcc ccccgaacga cagaagtgac cgtcgacgat ctgctcgcgg
     4441 tcggattcac ggacgatcag atcgaacggc ttcaggcgct caagaacgcc tatccgttta
     4501 tcgagtatgt cgacacggcc aagcaatggc gtcgtctcga atttctcaag tggcgctacc
     4561 tgcagggaga tctgcagagg gcgtaattcc tcttccgcat cccggcccgc gttcctcggc
     4621 cactcggaca gtccatcgtt cctgtttcct ggaaccatcc aacgtgtgga cagcgcgcga
     4681 cgcgcgtggt atcagcctgc ttgatagacc gtccggttcg tgtgggtgga ggaaacggtg
     4741 gcagcgcagg aacgcactcc agaggttgtg atcgtcggcg gcggcatcat cggctgctcg
     4801 ctggcgtggg aactggcgaa agcgggcgtg acatcccgtg tcatcgagcg gcgcgagatc
     4861 gcccgcgagg ccagctgggc gtcagcgggg atcatctccg ctcccggccc gcgacatggt
     4921 gcgcgcgcag gcctcgcgct cgacgcgttt cgccgctatc cggaactcat cgccgagatt
     4981 gaggacatca cgggccgctc ggtcggctac gtccgctccg gcgagctgct gctggcagac
     5041 aacgagactc tcgacgccgc ccgcgagacg ttcagttggc aacaggtcaa cggggtcgca
     5101 gtcga
//
LOCUS       DASUGJ010000244         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1980371, whole genome shotgun sequence.
ACCESSION   DASUGJ010000244 DASUGJ010000000
VERSION     DASUGJ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980371"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VFV93_05370"
     CDS             complement(<1..1071)
                     /locus_tag="VFV93_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HEX5164805.1"
                     /translation="MLLTITTTHQPATDLGYLLHKHPERVQSFALNYGQAHVFYPEAT
                     AERCTAALLLEIDPVGLARGRGRGGHALEHYVNDRPYVASSFLSVAIGDVFGTALAGR
                     CNARPELVEQRLPLTARLSALPCRGGEPFLRRLFEPLGYQVVAEHHPLDERFPEWGAS
                     RYFTVELSGTARLSDLLAHLTVLVPVLDDAKHYYVGTDEVEKLLRRGAGWLPEHPERE
                     EITRRYLRRRGSLVRDALERLIAEEHPAQATEAAEEAAEEETIEQRISLNQQRLDAVL
                     AALRESGAQRVLDLGCGEGRLLRLLLAERQFTEIVGMDVSHRILDSAADRLRLDRRPE
                     HQRERIRLIHGSLTYRDERLAGF"
     gene            1246..1560
                     /locus_tag="VFV93_05375"
     CDS             1246..1560
                     /locus_tag="VFV93_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164806.1"
                     /translation="MLVMVKCMIFEGSGELPAAGTAAPRITHVREGQVLGAWRATEGR
                     VGYALLDITSMAMVATLLKHAFPTARHIDVDEILAVEPSPAAGAQRRDRSSSGDSRQT
                     GT"
     gene            complement(1598..2164)
                     /locus_tag="VFV93_05380"
     CDS             complement(1598..2164)
                     /locus_tag="VFV93_05380"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5164807.1"
                     /translation="MTATRLMTAEEFWTMPEVPGKQYELVNGELVEMPTASHYHNLIV
                     FLIGKLVDAFASQHDLGYTFVDGVSFVLRRNPDDVRGPDVSFVATARVTSRDMLLKPW
                     EGAPDLAVEIISPSDRATDIDEKIKAYLSAGTRLIWVVWPDTQSVTTYELGGAITDLR
                     STDTLGGGDVLPGFAVSVATLFEVGPAR"
     gene            2353..>2563
                     /locus_tag="VFV93_05385"
     CDS             2353..>2563
                     /locus_tag="VFV93_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164808.1"
                     /translation="MATATPTRPTPSLESAPSSVRDAAQRLLRAADTPGKTAFVARAL
                     SALATIANELDERTLADAGGATSDVL"
BASE COUNT          436 a          857 c          828 g          442 t
ORIGIN      
        1 gaagcccgcc agccgctcgt cgcggtaggt cagcgagccg tggatcagac gaatccgctc
       61 gcgctggtgt tccggccgcc ggtcgagccg caggcggtcg gcggcactgt cgaggatgcg
      121 gtgcgagacg tccatcccga cgatctcggt gaactggcgt tcggccagca gcaggcgtag
      181 caatcggcct tcaccgcagc cgagatcgag gacgcgctgc gcgccggact ccctgagcgc
      241 ggccaggacg gcgtcaagcc gctgctggtt gagcgagatc cgttgctcga tcgtctcctc
      301 ttcggcagct tcttcagcgg cttcggttgc ctgggcaggg tgctcctcgg cgatcagtcg
      361 ctccaacgcg tcgcgaacga ggctgccgcg ccggcgcagg tagcggcggg tgatctcctc
      421 ccgctcgggg tgctccggca accaaccggc gccgcgccgc agtagcttct cgacctcgtc
      481 ggtgccgacg tagtagtgct tggcgtcgtc gaggaccggc acgagcaccg tcaggtgcgc
      541 cagcagatcg ctgagccggg ccgtcccgga cagctcgacg gtgaagtagc ggctggcgcc
      601 ccactccggg aatcgttcgt cgagtggatg gtgctcggcg acgacctgat accccaacgg
      661 ctcgaacagt cgccgcagga acggctcacc accccggcag ggcagggccg agagccgcgc
      721 cgtcaacggc agccgctgtt ccaccaactc cggccgcgcg ttgcaacggc cggcgagtgc
      781 cgtcccaaac acatcgccga tcgcgacgct caggaacgac gacgcgacgt agggacgatc
      841 gttgacgtag tgctccagcg catgtccgcc ccgtccacgg ccgcgcgcca gaccgaccgg
      901 gtcgatctcc agcaacagcg ccgctgtgca gcgctcggcc gtcgcctccg ggtagaagac
      961 gtgcgcctgc ccatagttga gtgcgaacga ttggacccgc tcgggatgct tgtgcagcag
     1021 gtagcccagg tccgtcgccg gctgatgggt cgtcgtgatc gtcaacagca tcgtgatcgc
     1081 tcccagcgcg tcgctcagat gctaccccgg ccgacagcgc ggctgccatc ggcttggcca
     1141 tgacgaccaa atgtagtact tccggggctg tcgcctggtc ggcgcgacat gcagactcag
     1201 acacagaagc gtgcgccgcc tggacgcgat gggacatcga agcagatgct ggtaatggtc
     1261 aagtgcatga ttttcgaagg gagcggcgag ctacccgctg ctggtactgc cgcgccacgc
     1321 atcacgcacg tccgtgaggg gcaggtgctc ggcgcctggc gggcaaccga gggtcgggtc
     1381 ggctacgcgc tgctcgatat cacctcgatg gcgatggtcg cgacactgct gaagcatgcc
     1441 ttcccgaccg cgcgtcacat cgacgtggac gagatcctcg ccgtcgaacc gtccccagct
     1501 gctggcgccc agcggcgcga ccggtcgtcg tcaggcgact cccgccagac gggtacgtag
     1561 cctgggtgtc ccggcggcgc catcgcacca agtacgtcta cctggctggc ccaacctcga
     1621 acagcgtcgc gactgagaca gcgaatcccg gcaggacatc gccgccacca agcgtgtctg
     1681 tggacctgag gtcggtaatg gccccaccga gttcgtaggt cgtcaccgac tgcgtgtcgg
     1741 gccaaacaac ccagatgagt ctcgtccctg ctgacaagta cgccttgatc ttctcgtcga
     1801 tatcggttgc gcggtcgctc ggcgaaatga tctcgaccgc gagatccgga gcaccttccc
     1861 acggcttcag gagcatgtca cggctcgtga cacgggccgt tgccacaaac gacacgtcgg
     1921 ggcctcgcac atcatccggg ttacgtcgca gcacgaacga aacaccatct acgaacgtgt
     1981 aaccaagatc gtgctggctt gcgaacgcat cgaccagctt tccgattaga aagacgatca
     2041 ggttgtggta gtggctcgcg gtcggcatct cgacgagttc tccgttcacg agctcatact
     2101 gcttgccggg cacctccggc atggtccaga actcttcagc ggtcatcagc ctggtggcgg
     2161 tcatgcgagc cttccgttcc ggatcgtcgt gcggtctgcg gtcctacatt cgccgcgatc
     2221 ataccaactt cgttgtccgc gactgatgtt ggagggcgtg caagcgcttc aacctggcac
     2281 gggcgtagga ggggctgagt tgcccaagtt gcggtggtgg actacactgt actctgacaa
     2341 caggaggtcg ctatggcaac tgcaacgccg accaggccca cgccatcact cgaatccgcg
     2401 ccgtcgtcag tccgtgacgc ggcccagcgg ctgctgcgcg ccgccgatac gccgggcaag
     2461 actgccttcg tcgcccgtgc cttgtctgcc ctcgccacga tcgccaacga gcttgatgaa
     2521 cggacactgg ctgacgctgg cggtgcgacg tccgatgttc tgg
//
LOCUS       DASUGJ010000245         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1424244, whole genome shotgun sequence.
ACCESSION   DASUGJ010000245 DASUGJ010000000
VERSION     DASUGJ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424244"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1423
                     /locus_tag="VFV93_05390"
     CDS             395..1423
                     /locus_tag="VFV93_05390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5164809.1"
                     /translation="MVSDGRLPESTDDPIATDSLWVTVLMPVYNGERFVSQAIDSILA
                     QTYPWFEFLIINDGSTDSTASILNDYASRDSRIRVIHQSNIDQPATLNRGLREASHDW
                     VAIIDHDDISRPQRLERQVLAVQQSPEVRAVGTYGYEITADGTFLGEMAFGPTTVREY
                     AARRSRNAWMSFIHASVLMHRPTILTLGGYREAFGSAADSDLWSRVADRHPIIVVPEY
                     LVDYRVHLNSMSFKRFFEQQRTVRWISACQEARRSGNPEPTLEELAIAERGPLYIGRF
                     NLQRYDWMMFCLRRRRLLRREGKAVRAAAYFMLAAVLDPVRATRRILGRMSRHDELPL
                     RQTTPAPR"
     gene            complement(1425..>2549)
                     /locus_tag="VFV93_05395"
     CDS             complement(1425..>2549)
                     /locus_tag="VFV93_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164810.1"
                     /translation="LERCFHKLLLNFTWWVNKVDRDGSNVFEGGFLGLDNIAVLDRSE
                     PLPEGAILEEADATGWMGMFCLNLMRIALELAKDNPVYEGLAVKFFEHFIYIGAAMKQ
                     MHRDGQPLWNAEDGFFYDLLRQADGSVIPFRVRSLVGLIPLYAVERLERDWMRPFDEF
                     RESVDWFLRNRLDLVEECVTTVTHDGDDVHVLSIVNQQQLRRILARIWNPDEFLSPYG
                     MRSLSKTHEHEPFVFGDRVVRYEPAEAITKIKGGNSNWRGPVWFPTTFMMIEAIRKLA
                     TAFGEDIDLGLLTADGRQVTFDDISSGLANRLISIFTKDDHGRRPVHGRHEIFQRDPD
                     WHDLLLFYEFFDGDTGVGLGASHQTGWTGLVASLIDEWRR"
BASE COUNT          455 a          828 c          802 g          464 t
ORIGIN      
        1 cagcgcggat tcggaacgac tcatgcgtgt ccttctcgct catcactcga tggccgaacg
       61 ccaccagtga gcaggaagcg atatcgttgt cggtgcctga ttcattgcca cgagccagac
      121 agcagcaact gccggactcc cttgcggccg ttgccgtcgc aactatagtg tccagctacc
      181 actggcgaca cctgactttc gtcgtaacgt ttaggcgagc ccgaatagat gggccagcca
      241 cagcaacttt tccataggct acactacatg tgtgcacagt ttgccgagcc gccggtagcg
      301 tcaaccgcgt gcggatcgag cgtgtggatg gacggtcgcc gcccgggcgc agcctgcacg
      361 acgacgcgag acgacgaaag gaccagaggg ccacatggtc tcggatggca ggctgccgga
      421 atcaacggac gacccgattg cgacggactc gctctgggtg acggtcctga tgccggtcta
      481 caacggcgag cggttcgtca gccaggcaat agacagcatt ctggcgcaaa cctatccgtg
      541 gtttgagttt ctgatcatca acgacggctc gaccgactcg accgcatcca ttctgaacga
      601 ctacgccagc cgggactcgc gcatccgcgt catccaccag agcaacatcg accagccggc
      661 gacgctcaac cggggactgc gcgaggccag tcacgactgg gtcgccatca tcgatcacga
      721 cgacatctct cgcccgcagc ggctggagcg gcaggtgctg gcggttcagc aatcacccga
      781 ggtccgcgcg gtcggcacct atggctacga gatcaccgcc gatggcacct tcctcggcga
      841 gatggcattc ggtccgacga ccgttcgcga atacgcggcc cgccgtagcc ggaacgcctg
      901 gatgtcgttc atccatgcct cggtgctgat gcaccgcccg acgattctga cgctcggtgg
      961 ctatcgtgag gcgttcggtt cggcggccga ctcggacctc tggagccgtg ttgcggaccg
     1021 gcatcccatc atcgtcgttc ccgagtatct ggttgactat cgcgtccatc tcaacagcat
     1081 gtcgttcaag cgcttcttcg agcagcagcg cacggtgcgt tggatcagcg cctgtcagga
     1141 ggcgcgccgg agcgggaatc ccgagccgac gctggaggaa ctggcgatcg ccgagcgtgg
     1201 gccgctgtac attggccgct tcaacctgca gcgctacgac tggatgatgt tctgcctgcg
     1261 ccggcgtcgc ctgctgcgcc gggagggcaa ggccgttcgg gctgccgcct atttcatgct
     1321 ggcagccgtc ctcgatccgg ttcgcgcgac gcgccgcatc ctgggccgca tgagccggca
     1381 cgacgaactc ccgctccgac agacgacacc cgcgccacgc tgatctatcg ccgccactcg
     1441 tcgatcagcg aggcgacgag gcccgtccag ccggtctggt ggcttgcgcc gagaccgaca
     1501 ccggtatcgc cgtcgaagaa ctcgtagaac agcagcaggt cgtgccagtc ggggtcgcgc
     1561 tgaaagatct cgtgtcggcc gtggacgggc cgacggccgt ggtcgtcctt cgtgaagatc
     1621 gaaatcagcc ggttggccag cccgcttgag atgtcgtcga acgtcacctg gcggccatcg
     1681 gccgtgagca ggccgaggtc gatgtcctcg ccgaacgccg tcgcgagctt gcggatcgct
     1741 tcgatcatca tgaacgtcgt cgggaaccag accgggccgc gccagttcga gttgcccccc
     1801 ttgatcttcg tgatcgcttc tgccggctcg tagcggacga cgcggtcgcc gaagacgaat
     1861 ggctcgtgct cgtgcgtctt cgacaggctg cgcatgccgt atggcgagag gaactcgtcg
     1921 ggattccaga tgcgggccag gatccggcgc agttgctgct ggttcacgat cgacagcacg
     1981 tgcacgtcat cgccgtcgtg cgtcacggtc gtgacacact cctccactag gtccagccgg
     2041 ttgcgcagga accagtcgac gctctcgcgg aactcgtcga acggccgcat ccagtcgcgc
     2101 tccagccgtt cgacggcgta gagcgggatc agcccgacca gtgagcggac gcggaaggga
     2161 atgacgctcc cgtccgcctg gcggagcagg tcgtagaaga aaccatcctc cgcgttccag
     2221 agtggctggc cgtcgcggtg catctgcttc atggctgcgc cgatgtagat gaagtgctcg
     2281 aagaacttga cggccagccc ttcgtagacc gggttgtcct tcgccagctc cagcgcgatc
     2341 cgcatcaggt tgagacagaa catccccatc cagccggtgg cgtccgcctc ttcgaggatc
     2401 gcgccttccg gcagcggctc gctgcggtcg agaacggcga tgttgtcgag accgaggaac
     2461 ccgccttcga agacgttgct accgtcgcgg tcgaccttgt tcacccacca ggtgaaattc
     2521 agtagtagct tgtggaagca gcgttccag
//
LOCUS       DASUGJ010000246         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_35732, whole genome shotgun sequence.
ACCESSION   DASUGJ010000246 DASUGJ010000000
VERSION     DASUGJ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35732"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /gene="extP"
                     /locus_tag="VFV93_05400"
     CDS             <1..712
                     /gene="extP"
                     /locus_tag="VFV93_05400"
                     /inference="COORDINATES: protein motif:HMM:NF040969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HEX5164811.1"
                     /translation="IADRVTSSALWRSIVRHGYPDTQRNQALIVASNVFLHLHPVKIK
                     RYATKVTYTFCLGGLSFFMFLILVMTGVMLMFYYVPATDQAYQNMQDIEASVTFGQLM
                     RNMHRYAAHGMVIAVFLHMCRVFYTGSYKPPREFNWVVGVILLFLTFLLSFTGYLLPW
                     DQLAYWAITVGTTMAEAAPLIGPKVRFLLVGDIEIGQSTLIRFYTLHVIFLPLAAAVM
                     MAVHFWRIRKDGGISGPL"
     gene            764..1543
                     /locus_tag="VFV93_05405"
     CDS             764..1543
                     /locus_tag="VFV93_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164812.1"
                     /translation="MAEAVRRPGGGVQTGNISEEKRQRLLELVRGRTAARPTDLAEDE
                     VMVWPTLVVVEALSAVIFLFLLLIASIAINPPLVDVANPTVTPNPSKAPWYFLNLQEL
                     LLHMHPSLAGVIVPTVALLLIAVIPYVDRSPLGVGILFTSDKGKSITIFSAIFTTVIL
                     IGLILFDQYVGVTKSLTGKVDPLIYEQIIPVAIMVGFSVILAVLVSLRYKPTRRELII
                     ALFTGFVTSYLVLTISGTFFRGYGMELQLYWPWTDLHAAAH"
     gene            1608..2183
                     /locus_tag="VFV93_05410"
     CDS             1608..2183
                     /locus_tag="VFV93_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5164813.1"
                     /translation="MFALARTLVGMLLASIIGKLVLLLSRFHQTTPQRLVSRRSFLRN
                     AVLGSVGVSLIGIAGGFVTFFWNNKTGAFGGEIPVPLDLVPAVGAAPYRNQAGKFYII
                     NNEDGALALYWKCVHLGCTVPWNEGEGDFHCPCHGSVYNRFGERIAGPAPRPLDLFPM
                     KLDGSNVIVDTDPAGAIVRHSYEPSQALKLT"
     gene            2264..3049
                     /locus_tag="VFV93_05415"
     CDS             2264..3049
                     /locus_tag="VFV93_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5164814.1"
                     /translation="MGASQRLATVVAIGLVIVATFSVVYLFNEPDRRDTAAADKLHES
                     ALIGVETYVANCLVCHGPDGKAEGRQGIPLNTDQNQVTGANWDLREPVIRRTIERGRG
                     QVMPAWAQSEDGPLNDQQITGLVNMIHLGYWDEVEKEAKAANGGQLPTPPPLPTAAGG
                     TPEDPAAAAGQQLATDNGCVACHTVDGTDATGPTWKGLFGHEVKLADGSTVTADEAYI
                     TESIHDPSANVVEGFSDIMPKTYANMSQEDVDAIIAYIKSLSE"
     gene            complement(3419..3781)
                     /locus_tag="VFV93_05420"
     CDS             complement(3419..3781)
                     /locus_tag="VFV93_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164815.1"
                     /translation="MLEIHSALYTTLLILSGGLALWALFLMATGRPIDGAYRSTYVLT
                     IGVSVAQAAVGLMLYLDDHRPAESFHYLYGISLMVFTGAGYAFASRGPSNREPLVFGI
                     ASAAAVGLIWRAWYTAHG"
BASE COUNT          723 a         1132 c         1229 g          767 t
ORIGIN      
        1 gatcgccgat cgagtcacga gcagcgcgct gtggcgatcg atcgttcgac atggctaccc
       61 tgatacgcag cgcaaccagg cgctgattgt cgcatccaac gtcttcctcc acctgcaccc
      121 agtcaagatc aagcgctacg caacgaaggt gacctacacc ttctgcctcg gcgggctgtc
      181 tttcttcatg ttcctgattc tggtcatgac gggcgtcatg ctgatgttct actacgtgcc
      241 ggcgaccgac caggcgtatc agaacatgca ggatatcgaa gcatcggtca cgttcggcca
      301 actgatgcgg aacatgcatc gctacgctgc ccacggcatg gtgattgccg tcttcctgca
      361 tatgtgccgc gtgttctaca cggggtcgta caagccgccg cgggagttca actgggtagt
      421 gggcgtcatt ctgttgttcc tgaccttcct tctctcgttt actggctacc ttctgccgtg
      481 ggaccagctc gcgtactggg cgatcaccgt cggcacgacg atggccgagg cggctccgct
      541 gattggacca aaggttcgct tcctgctggt cggcgacatc gagatcgggc agagtacgct
      601 gattcggttc tataccctgc acgtgatctt cctgccactt gcggcggcgg tcatgatggc
      661 ggtccacttc tggcgcatcc gcaaggatgg tgggatctcc ggcccgctct agaagcggta
      721 accgtagcgg ggcgtgtgat tcgcggcgaa agagggtagg ccagtggcag aggcagtacg
      781 acgaccaggt ggtggcgtcc agacaggcaa tatcagcgag gaaaagcggc aacggctgct
      841 ggaactcgtg cgtgggcgaa ctgctgcccg cccgaccgac ctcgccgaag atgaggtgat
      901 ggtctggccg acgctggtcg tcgtcgaggc actcagcgcg gtgatcttcc tgttcctgct
      961 gctgatcgcc tcgatcgcca ttaaccctcc cctggtcgat gtcgccaacc cgaccgtcac
     1021 accgaaccca tccaaggcgc cgtggtactt cctgaacctg caggagctgt tgctgcacat
     1081 gcacccgtcg ctggcaggtg tgatcgtgcc gacggtggcg ctgctgctga ttgcggttat
     1141 cccctatgtt gatcgctcgc cgttgggtgt cggcatcttg ttcacgtccg acaagggcaa
     1201 gtcgatcacg atcttctcgg cgattttcac gaccgtgatc ctgatcggtc tgatcctgtt
     1261 cgatcagtac gtcggcgtaa cgaagagcct gaccggcaag gtcgatccgc tcatctacga
     1321 gcagatcatt ccagttgcga tcatggtcgg gttctcggtg attctggcgg tgctcgtctc
     1381 gctgcgctac aagccgacgc ggcgcgaatt gatcatcgcg ctcttcacgg gcttcgtgac
     1441 ctcgtatctc gtcctgacga tttcgggtac gttcttccgt ggctatggga tggaactgca
     1501 gctctactgg ccgtggaccg atctacacgc agccgcgcac tagtgattga gcggcccgat
     1561 tgggccggcg cggggcgtcc cgcgcgtact gtgaatgagg gagttgaatg ttcgccctgg
     1621 ctcggacgtt agtgggcatg ctgctggcgt cgatcattgg aaaactggtc ctgctgttga
     1681 gtcgcttcca ccagacgacg ccgcagcggc tcgtctcacg gagatcgttc ctgcgcaacg
     1741 cggtgctcgg ctccgtcggc gtctccctga tcgggatcgc cggtggtttc gtcacgttct
     1801 tctggaacaa caagacgggc gcattcggcg gtgagattcc agtgccgctg gacctggttc
     1861 ctgcggttgg cgcagcgccg taccgcaatc aggccggcaa gttctacatc atcaacaacg
     1921 aggacggcgc gcttgcgctt tactggaagt gcgtccacct gggatgcacc gtcccgtgga
     1981 acgagggcga aggcgacttc cactgtccat gtcatgggtc ggtttacaat cgcttcggcg
     2041 agcggatcgc cggtcccgcg ccacgaccgc tcgacctttt cccaatgaag ctcgatggca
     2101 gcaatgtcat cgtcgacaca gatccggccg gggcgatcgt gcgccattca tatgagccgt
     2161 cacaggcgct caagctgacg tagacgatag atgtttcgta acccgcgagg ttaaccgacg
     2221 gttggccggc aggccgtagg ctggtaggaa gagacgagga cgagtgggag cgtcgcagag
     2281 attagcgact gtagtcgcaa ttggactggt catcgtcgcg acattcagcg tcgtctatct
     2341 tttcaacgag ccggaccgac gagacaccgc tgctgcagac aagctccatg agtcggcgct
     2401 gatcggtgtc gaaacgtacg tggccaactg tctggtgtgc cacgggccgg atggcaaagc
     2461 ggagggccgc cagggtattc cgctcaacac cgaccagaac caggtgactg gggcgaactg
     2521 ggacctgcgc gagccggtga tccggcgcac gatcgagcgc ggtcgcggtc aagtcatgcc
     2581 cgcctgggcg caaagcgaag atggccccct gaatgaccag cagatcactg gcctggttaa
     2641 catgattcac ctcgggtact gggacgaggt cgagaaggag gcgaaggcag ccaacggtgg
     2701 ccagcttccg accccgccgc cgctgccgac tgccgctggc ggaacgccgg aggacccggc
     2761 cgccgcagct ggacagcaac tcgcaacgga caatggttgt gtcgcctgcc acaccgtcga
     2821 cggtactgat gcgaccgggc cgacctggaa ggggctgttt ggccacgagg tgaagctggc
     2881 ggacggcagc acggtgacgg cggacgaagc ttacatcact gagtcgatcc acgatccgag
     2941 tgcgaatgtc gttgaaggat tctcggacat catgccgaag acgtatgcca acatgtccca
     3001 ggaagacgtc gatgcgatta tcgcctacat caagtcgttg tcggaatagt aaccggctgg
     3061 gaggacgcgg accatgtttt accgggcgac cgaggcgatc gagttgttgc ttgtcttccc
     3121 gctgatgatc atcggcatcc tgctgacaca cctgatccaa cgctgggcgg acaagagcgc
     3181 tggcgaagag taagcgccgg gaatcgagaa tcgaaacgac ggggccgata ttcggccccg
     3241 tcgttgtgcg actgacatga ccctctcgga ggcagcgagt ccaatcctca atccggtacg
     3301 ggccatatcg caataccgcc ctttggttcc acccgaacgc gtcggacggg tagcacggcg
     3361 atattgcgat atggcccgta ccggattgtg aaattgtgga attgctgggc gatcgggact
     3421 acccgtgggc ggtgtaccag gcgcgccaga tgagtccgac ggcggcggcc gaggcgatgc
     3481 cgaagacgag tggctcgcgg ttagatggac ctcgcgaggc gaaggcgtag cccgcgccgg
     3541 tgaagaccat caggctgata ccgtacaggt agtgaaagct ttctgccggc cggtgatcgt
     3601 ccaggtacag catgaggccg acggccgcct gtgcgacaga gacgccaatc gtcagtacat
     3661 aggtcgagcg ataggcgccg tcgatcggcc ggcccgttgc catcaggaac agcgcccaga
     3721 gcgcgagccc gcccgataga atcaggaggg tggtatagag ggccgagtgg atctccagca
     3781 cgtgttcgtc ccttccgggt tatcgctgac gtggcgcacg gatgcgaccg ttcgatcggc
     3841 tgcctggcag a
//
LOCUS       DASUGJ010000247         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1565507, whole genome shotgun sequence.
ACCESSION   DASUGJ010000247 DASUGJ010000000
VERSION     DASUGJ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565507"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFV93_05425"
     CDS             complement(<1..666)
                     /locus_tag="VFV93_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5164816.1"
                     /translation="MLVIALLAVPLVTGISCRLLWKRQRLIEALNVVGALLTLALGGA
                     VVVEVARDGWFTALRGTLYVDALSAVMVGSIVVVGAAAALVSVNYMRHDLAERIVPEG
                     RRGLGWYYLGLHAFIFTMLATVLVDNLGLLWVGVEATTLASALLVGFYRTKAAIEAAW
                     KYVILCTVGITFALFGVLLTYYASVQSGGGRGLDWSELSLRAGQLDGDMMRLAFVFIL
                     IGFG"
     gene            complement(725..1411)
                     /locus_tag="VFV93_05430"
     CDS             complement(725..1411)
                     /locus_tag="VFV93_05430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HEX5164817.1"
                     /translation="MDAFISVEHAGQIIDAAAIGLLALAVVGVVVRRLELAIILLAGQ
                     GVLLGVASGAAALAEMEWRPWAAFSVALAVKAVAIPVILWFMLERVALRHEAETVVPL
                     KLAFPIALGLALLAYWVAEPFTGPALAAEHTFAARNALPAALGLLLLGLFTMASRKKA
                     ITQVIGLVTMENGIYLAAVAATRGLPFAVEFGIALDVLTGVALMALVTHEINRLFSTI
                     NTDRLRSLRG"
     gene            complement(1420..2337)
                     /locus_tag="VFV93_05435"
     CDS             complement(1420..2337)
                     /locus_tag="VFV93_05435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX5164818.1"
                     /translation="MLLLINVLIMLFLAPLLQGVIKTTKARMQGRRGPGILQPYRDIL
                     KWLGRESVVSEHASWVFRLAPMVYAAAILLAALLVPTVAERSPLPGVADAIVFVGLLA
                     LARFWLAIAAIDTASNFGGMGASREVAFAALVEPALLLVLFAVALPIGSTSLTALVGD
                     GSPGVGDLLALGALLVVVVAETGRIPIDNPDTHLELTMIHEGMLLEYAGRPLGVLHWA
                     TQVKQLAFLSLLAALFFPWGMADGGSTDVAALGLGLASWMLKVAVLGLLLAAIETAYA
                     KLRIFRAPDLIGVASVLGVLAVIATYVVE"
     gene            complement(2395..>3888)
                     /locus_tag="VFV93_05440"
     CDS             complement(2395..>3888)
                     /locus_tag="VFV93_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5164819.1"
                     /translation="TGFLLLAFLGLVRHTGSFDFSAFRAAAASLGGWERDVIFLLALL
                     GFGTKAGLIPLHIWLPRAHPAAPSHVSALMSGVMVKTAIYGLARIVWEFAGPGPSWWG
                     WLLIAAGAGSAVLGILYALMERDIKRVLAYSTVEQVGIITVGLGVATLATANGHPAVA
                     ALAITAALLHLLNHSVFKGLLFLGAGAIQVGTGTRDLERLGGLVRRMPWTAGLFLTGA
                     LAIASLPPLNGFAGEWLLFQSLLGLGSADGTASVATIVALAVGAVALTAGLSVACFVR
                     AFGIGFLAQPRSDAARDAHEVSRSMLAGMALLAGCAIGLGLFPIAIVRLLRPVTTDLV
                     GMTASPSLGNGDVLDATQLGGAYAPWLVVAGLIAVGIVPWLLARLVGGRGRERVAPTW
                     VCGGTLEPRMQYSATGFAKPIRLIFQALIRPVRTVVLGRPVSEHFVASVRYEESVLPV
                     YERLFYERGVGLLVAASHRVRALQSGSIRAYLAYLLVTLVVVLFVAR"
BASE COUNT          767 a         1446 c         1226 g          449 t
ORIGIN      
        1 cccaaacccg atcaggatga agacgaacgc cagccgcatc atgtcgccgt cgagctggcc
       61 ggcgcgcagg ctcagctcgc tccagtccag accacgcccg ccaccggact ggaccgaggc
      121 gtagtaggtc agcaggacac cgaacagcgc gaaggtgatc ccaaccgtac agaggatgac
      181 gtacttccag gccgcctcga tggccgcctt cgtccggtag aagccgacca gcagggccga
      241 agccagcgtc gtcgcctcga cgccgaccca gagcaatccc aggttatcga ccagcaccgt
      301 cgccagcatc gtgaagatga acgcgtgcag gccgagatag taccagccca gcccgcgacg
      361 tccctccgga acgatccgct cggccaggtc atgccgcatg tagttgaccg acaccagcgc
      421 cgctgccgcg ccaacgacga cgatcgagcc gaccatcacg gccgagagcg cgtcgacgta
      481 gagcgtcccg cgcagcgccg tgaaccaccc gtcgcgcgcg acctcgacga cgacggcccc
      541 gcccagtgcc agtgtcagca gcgccccgac gacattcagc gcctcaatca gccgctgccg
      601 cttccagagc aggcggcagc tgatgccggt cacgagtggc actgccagta acgcgatcac
      661 cagcatggcg ctccccgatc gtcgggagca acgggcgccc cgatctgctg ccttatcatc
      721 cctctcaccc tcgtagcgac cgcaggcggt cggtgttgat cgtgctgaac agccggttga
      781 tctcgtgggt cacgagcgcc atcagcgcca ccccggtcag cacatccagc gcgatcccga
      841 actcgacagc gaagggcagc ccgcgtgttg cggcgaccgc cgccaggtag atgccgttct
      901 ccatcgtcac caggccgatc acctgggtga tcgccttctt gcgtgatgcc atcgtgaaca
      961 ggccaagcag cagcagcccc agcgccgccg gcagcgcatt gcgcgccgcg aaggtgtgct
     1021 cggcagccag ggccggcccg gtgaacggct cggccaccca gtaggccagc agcgccagcc
     1081 ccagcgcgat cgggaaggcc agcttgagcg gcacgaccgt ctcggcctcg tgccgcaacg
     1141 cgacccgctc cagcatgaac cagaggatga ccgggatggc aacggccttg accgccagcg
     1201 cgacactgaa ggcggcccac ggtcgccact ccatctccgc cagcgccgcc gcgccggatg
     1261 cgactcccag caacaccccc tgcccggcca gcaggatgat cgccagttcc agccgcctca
     1321 cgacaacgcc aacgaccgcc agcgccagca gcccgatcgc cgccgcgtcg atgatctgcc
     1381 ctgcatgttc gaccgagatg aacgcgtcca tcgtctccgc tactccacga cgtaggtcgc
     1441 gatgactgcc agcaccccga ggacgctggc aacgccgatc aggtccgggg cgcggaagat
     1501 gcgcaacttg gcgtaggccg tctcgatcgc cgccagcagc aggccgagca ccgcgacctt
     1561 cagcatccag ctcgccagcc caagccccag agccgcgacg tcagtgctgc cgccgtcggc
     1621 catcccccat gggaagaaga gcgccgccag cagtgataga aaggcgagtt gcttgacctg
     1681 ggtcgcccag tgcaggacgc cgagcggccg gccggcatac tccagcaaca tgccttcgtg
     1741 gatcatcgtc aattccaggt gggtgtccgg gttgtcgatc gggatccggc cagtctcagc
     1801 gacgacgacg accagcagcg cacccagcgc cagcaaatcc ccgacgcccg ggctgccgtc
     1861 accgaccagc gccgtcagcg aggtcgagcc gatcggcagg gcaaccgcga acagcaccag
     1921 cagcaacgca ggctcgacga gcgccgcgaa ggcaacctcg cggctggcac ccatcccgcc
     1981 gaagttgctg gccgtgtcga tggcggcgat cgccagccag aagcgcgcca gcgccagcaa
     2041 cccgacgaag acgatcgcat ccgcgacacc cggcagcggc gagcgctcgg cgacggtcgg
     2101 cacgagcaat gccgccagca ggatcgccgc cgcatagacc atcggcgcca ggcgaaatac
     2161 ccaggacgcg tgctccgaga cgacgctctc gcgtccaagc cacttcagga tgtcgcggta
     2221 cggctgcagg atgcccggac cgcgccggcc ctgcatccgc gccttcgtcg tcttgatgac
     2281 gccctggagc agtggcgcca ggaacagcat gatcaggaca ttgatgagca gcagcaacgg
     2341 atccatctgc taccggcccg cctgccggtc gaacgctacc tgcatcccac ccacctatcg
     2401 cgccacaaac agaacgacga ccagcgtcac aagcaggtag gcgaggtagg cgcgaatcga
     2461 gccgctctgc aacgcccgca cgcggtgcga ggccgcgacc agcagcccga cgccgcgctc
     2521 gtagaacagc cgctcgtaga ccggcaggac actctcctca tagcgaaccg acgcgacgaa
     2581 gtgctcggag accggccgcc cgaggacaac cgtccgaaca ggacgaatca gcgcctgaaa
     2641 gatcagccgg atcggcttgg cgaaacccgt cgccgagtac tgcatgcgtg gctccagcgt
     2701 gccgccacag acccaggttg gcgcgacccg ctcgcgcccc cggccgccga ccagtcgcgc
     2761 cagcagccac ggcacgatcc cgacggcgat cagtccggca acgaccagcc acggcgcgta
     2821 ggcgccaccg agctgggtgg catccagcac gtcgccgttg ccgagcgagg gcgacgccgt
     2881 catgccgacc agatcggtcg tgaccggccg cagcagccgg acgatagcga tcgggaacag
     2941 ccccagaccg atggcacagc cggccagcag cgccatccca gccagcatcg agcggctgac
     3001 ctcgtgggca tcccgcgccg cgtcactgcg cggttgggcc agaaagccga tcccgaacgc
     3061 gcgtacgaag caggcgaccg acagcccggc cgtcaacgcg accgccccga cggccagcgc
     3121 gacgatcgtc gcgacactgg cggtcccgtc agccgagccg agaccgagca gcgactgaaa
     3181 caggagccat tcgccggcga agccgttcag cggcggaagc gaggcgatcg ccagcgcgcc
     3241 ggtcaggaac aggccggccg tccacggcat ccgccgcacc agcccgccca gccgttcgag
     3301 atcgcgcgtg ccggtcccca cctgaatggc gccggcgccg aggaagagca ggcccttgaa
     3361 gacgctgtgg ttgagcaggt gcagcagcgc ggccgtgatc gccagcgccg caaccgccgg
     3421 atgcccgttg gccgtcgcca gcgtcgcgac accgaggcca accgtgatga tcccgacctg
     3481 ctcgacggtc gaataggcca gcacgcgctt gatgtcgcgc tccatcagcg cgtagaggat
     3541 gccgagcacg gcagatccgg cgccggcggc gatcaggagc cagccccacc acgacggccc
     3601 cgggccggcg aactcccaga cgatgcgcgc cagcccgtag atcgccgtct tgaccatgac
     3661 gcccgacatc agcgccgaga catgactcgg cgcagccggg tgtgcacgcg gcagccagat
     3721 gtggagcgga atcagcccgg ccttcgtccc gaagccaagc agcgccagca ggaagatgac
     3781 atcccgctcc cagccgccca gcgaggccgc tgcagcgcgg aaggctgaga agtcgaagga
     3841 gccggtgtga cggaccagtc ccaggaacgc cagcagcaga aacccggt
//
LOCUS       DASUGJ010000248         3778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1981386, whole genome shotgun sequence.
ACCESSION   DASUGJ010000248 DASUGJ010000000
VERSION     DASUGJ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981386"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1009)
                     /locus_tag="VFV93_05445"
     CDS             complement(44..1009)
                     /locus_tag="VFV93_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164820.1"
                     /translation="MTASANRHRGARLIVALLLLGSALLAGILPAAAQRPNGAGIVIN
                     FGDGSILYAYVQFDEGSITSEELLYRSGLAVVVAPYSGLGAGVCMIRDVGCPSDNCFC
                     KSYTTPAYFWHFYGYDGGWVELLQGVSSRSVGDGDIDGFAWTAGDSGLPDVTIDEIAQ
                     LNGVDRNPSAPTPTETPTLAPTDTPPPPTSTPPPTATQPASTSTPNIAATATSPAVPT
                     ATATISATSTATAAIGQVAVTPSPTREAAPTRPPAAAPSPTLTLVASATTLAVIVQPD
                     GTAVPVEVIQTDDGSSMTNLLLFGGLVVVAAGAGAVVWLRNRRVV"
     gene            complement(990..2540)
                     /locus_tag="VFV93_05450"
     CDS             complement(990..2540)
                     /locus_tag="VFV93_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5164821.1"
                     /translation="MVNRYGRSLPVALLLVVLLALSAAPALSQRAPAATPQPDAANAA
                     LDWLRTQQQPDGGFSAFGGASDPSVTADVVYAFAAAGINAGSLFAPSGAFTEDYLLHA
                     AQSVASDPGKAAKLVLVFHILGEDPNNAGGAGLVEAISQSLNAQTGWYGISFYGHSLA
                     VLALASQDVDIPDAAIDAILTAQTPEGSWGFNGDPTAGTGDSNSTAIAIQALVTVDGD
                     RDAINRGLDYLRSLQNAGTGAIAYDTASVNDPGGDANSTALAIQAFVAAGEDPSTLEH
                     GDLLTALLTFQNESGAFQFQPAFPDDSLLATAQAVPALLLKAFPYEPLPELSPLSRAT
                     APAAPDARCAYQDVTQHNLCEPFAAYWHANGGLANFGYPLTEAFDDNGRTFQYFERAI
                     FELHPENAGTPYEVLLTRVGWDAYLLAHGGAMTGADPIGGECLYFNETTHNLCGGFGA
                     FWEQFGGLAVFGYPISEEMQEDGMTVQYFERARFEWHPGDWPERYDVLLGRLAADALD
                     RELDRVGQ"
     regulatory      complement(2671..2808)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2896..3309
                     /locus_tag="VFV93_05455"
     CDS             2896..3309
                     /locus_tag="VFV93_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164822.1"
                     /translation="MVQFIDDKPSRRLRRPHRRHFLVHVSRASKPKARNTVIYPWVDV
                     TGDVRDINEGLGVRIGNTVSVNGRTYGIEPSTTLYPIAGPGFAFLDRGSYKALGYYNE
                     IGITDLAEEAMDRTLISSQSREHARSVLEIGDRDE"
     gene            3306..3761
                     /locus_tag="VFV93_05460"
     CDS             3306..3761
                     /locus_tag="VFV93_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164823.1"
                     /translation="MIWQDFLITRDVPEQEIAAALATVFNVAQDRVLVVEDLMTYPGV
                     LTDEIGIRCERQSATGDFVLSLTIYLLNKDFEQFVEAHDDIDLIGRMCRIWDCAALVS
                     DDSLNPYSWLLVRGPDDVQPAHVDAESLDDDIWVLTAVPEAAGASPPMD"
BASE COUNT          662 a         1214 c         1268 g          634 t
ORIGIN      
        1 ccagcattgg gagagggggg ctgggggggt gagggctgcc ctgtcatacg actctccggt
       61 tgcgcagcca gacaaccgcc ccagccccgg ccgccacaac gaccaacccg ccaaatagca
      121 gcaggttggt catcgacgag ccgtcgtcgg tctggatcac ttcgaccggg acggcggttc
      181 cgtccggctg gacgatgacg gccagcgtcg tcgcgctggc aaccagcgtc agcgtcggcg
      241 acggagcggc ggccggcggc ctggtcggcg cggcctcgcg cgtgggcgag ggcgtcacgg
      301 ccacctgacc gatcgcagcg gtcgcggtcg atgttgcgga gatcgtcgcc gtcgcggttg
      361 gcacggcggg tgaggtcgcg gtcgcggcga tgttcggcgt gctggtcgag gctggctggg
      421 ttgcggtcgg tggtggcgtg ctggttggcg gtggtggggt gtcggtcggt gccagcgtcg
      481 gcgtctcggt tggtgtcggc gccgacgggt tgcggtcgac gccgttgagt tgcgcgatct
      541 cgtcgatcgt gacatccggc aggccggagt cgccggccgt ccaggcgaag ccgtcgatgt
      601 cgccatctcc gacgctgcgg ctcgagacgc cctggagcag ttcgacccag ccgccatcgt
      661 agccgtagaa gtgccagaag taggctgggg tcgtgtacga cttgcagaag cagttgtcgg
      721 acgggcagcc gacgtcgcgg atcatgcaga ctccagcgcc gagcccgctg tagggcgcga
      781 cgacgacggc cagccccgag cggtagagca gctcctcgct ggtgatcgag ccctcgtcga
      841 actggacata ggcgtacagg atcgagccgt cgccgaagtt gatgacgatc ccagcgccgt
      901 tgggacgctg ggccgccgcc ggcaggatgc ccgccagtag cgccgagccg agcagcagga
      961 gcgcgacgat cagccgcgct ccccgatgtc tattggccga cgcggtcaag ttcacggtcc
     1021 aacgcatccg ccgccagccg gccgagcagc acgtcgtagc gctccggcca gtcgccgggg
     1081 tgccactcga agcgcgcccg ctcgaagtac tggacggtca tcccatcttc ctgcatctcc
     1141 tcgctgatcg gatagccgaa gacggccagc ccgccgaact gctcccagaa tgcgccgaag
     1201 ccaccgcaga ggttgtgggt cgtctcattg aagtagagac actccccgcc gatcgggtcg
     1261 gctcccgtca tcgcgccccc gtgggccagc aggtaggcat cccagccgac gcgcgtcagc
     1321 aggacctcgt acggcgtccc ggcgttctcc gggtgcagct cgaagatcgc ccgctcgaag
     1381 tactggaagg tccgaccatt gtcatcgaac gcttccgtca gcggatagcc gaagttcgcc
     1441 agcccgccgt tcgcgtgcca gtaggccgcg aacggctcgc agaggttatg ttgcgtgaca
     1501 tcctgatacg cgcagcgagc atcgggcgca gccggagccg tggcgcgaga gagcgggctc
     1561 aactcgggca gtggctcgta cgggaacgcc ttcagcagca gagccgggac ggcctgcgcc
     1621 gtcgccagca gcgagtcgtc ggggaacgcc ggctggaact ggaacgcgcc gctctcattc
     1681 tggaacgtca gcagcgctgt caacaggtcg ccatgctcca gcgtcgatgg gtcttcgccg
     1741 gccgcgacga atgcctgaat cgccagcgcc gtcgagttgg cgtcgccacc gggatcgtta
     1801 accgaggcgg tgtcgtaggc gatcgcgcca gttcccgcgt tctgcaacga gcgcaggtaa
     1861 tcgaggcccc ggttgatggc gtcgcggtcg ccatccactg taaccagcgc ctggatcgct
     1921 atcgccgtcg agttcgagtc accggttccg gccgtggggt cgccgttgaa gccccacgag
     1981 ccttccggcg tctgggcggt cagtatggca tcgatcgccg catcgggaat gtccacgtcc
     2041 tgcgaggcca gcgccagcac cgccagcgag tgaccgtaga acgagatgcc gtaccagccg
     2101 gtctgggcgt tcaggctctg gctgatcgct tcaaccagcc cagccccacc ggcgttattc
     2161 ggatcttccc caaggatgtg gaacaccagg acaagcttgg cagccttgcc gggatcagag
     2221 gcgactgact gcgcggcgtg cagcaggtaa tcctccgtga aggcaccact cggggcgaag
     2281 aggctgccgg cgttgatacc ggctgccgcg aaagcgtaga cgacatcggc cgtgacgctc
     2341 gggtcgctgg cgccgccgaa agccgagaag ccgccgtccg gctgctgctg ggtccgtagc
     2401 cagtcgagag cggcgttggc ggcgtcgggt tgcggcgtgg cggccggcgc ccgttgggac
     2461 agggccggtg ctgcggagag cgccaacagc acgacgagga gcagggcgac gggcaacgag
     2521 cgcccgtaac ggttgaccac ggggtccccc tctcgatccg gccgcgccct ggtccggggc
     2581 gaggcggcat ccaacaaaaa cccgccttcg atcgcgcggg tgagagaggg aggtcggttt
     2641 gctgaccatc ccagcccact cgtgcgcgaa gcgaaccgga acggcaacgg caggtgacct
     2701 ggctccgcgc atgacgcgcg ttacagttgc ggcacagcac cggtctcgca ccggtttccc
     2761 cctgtgaccc cgcccgtcag ggcgagtacc cgctgccgga ttctgggttg tcacgccggc
     2821 tcaccggcgc ttggggtagt ctgtcatgca cgcgaacgcg cgtcaactcc gaatagtcga
     2881 taaaggaacc tgctcgtggt gcagttcatc gatgacaaac cctcgcgacg gttacgtcgc
     2941 ccgcatcgcc ggcacttcct cgtccatgtc agccgtgcga gcaagccaaa ggcgcgcaac
     3001 accgtgatct acccatgggt cgatgtgact ggggacgttc gtgacatcaa cgaagggctg
     3061 ggcgttcgga ttggaaacac cgtcagcgtc aacgggagaa catacggcat cgaaccgtcc
     3121 acgacgctgt acccgatagc ggggccgggc ttcgcgttcc ttgatcgagg ctcctacaag
     3181 gcactgggat actataatga aatcggtatc actgatcttg cggaagaagc aatggaccga
     3241 actctcattt catcccagag ccgcgagcac gcaagatccg tcctggaaat cggagatcgt
     3301 gacgaatgat ctggcaagac tttctcatca cacgcgacgt acctgaacaa gagattgcgg
     3361 cggcgctggc gacggtgttc aacgtcgcgc aagaccgtgt gctggttgtc gaggacttga
     3421 tgacctaccc cggcgtactg accgacgaga tcggaattcg ctgcgaacga caatcagcca
     3481 ctggagattt cgtcctgtcg ctgaccatct atctcctcaa taaggatttc gagcagttcg
     3541 tggaagcaca cgacgacatc gatcttatcg gtcgcatgtg ccgcatctgg gactgcgcgg
     3601 cgctcgtcag cgacgattcc cttaacccct actcatggct cctcgttcgt ggaccggacg
     3661 atgttcagcc cgctcatgtt gacgcagaat cgctcgacga cgacatctgg gttctcaccg
     3721 cagtcccgga agctgccggg gcgtccccac caatggactg aacctgctcc atgacacc
//
LOCUS       DASUGJ010000249         6080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1286829, whole genome shotgun sequence.
ACCESSION   DASUGJ010000249 DASUGJ010000000
VERSION     DASUGJ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286829"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFV93_05465"
     CDS             complement(<1..866)
                     /locus_tag="VFV93_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164824.1"
                     /translation="MSAMLATSESDITFLTTEQVVLPEPPARRAEPVAAPALSGWLAD
                     MIREERDGYALLSSAGQTVVLLYAGGRLVETAQTRGGDAVGAQALHDLLDGEQGEWFV
                     VSHPVDEGLARCMAGLFSPPSSLSPLTLAGGSLDTILARLRRERFSGALRLTVTEGYA
                     VVVLVADGAPLASYSTDERILKADLADITFLAAFDDLSIAVHPAPVRDLASILDAARI
                     AQPADAGSEGRDALIVESLLIEAFSTLEYEISVAELGAPTLEHLSVALAGVYGLLADA
                     QIVIGRPSAEQAP"
     gene            complement(866..1270)
                     /locus_tag="VFV93_05470"
     CDS             complement(866..1270)
                     /locus_tag="VFV93_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5164825.1"
                     /translation="MQDRAVGDITVLAVDDSASIRKLVELTLRREGYSVVTADSGLKA
                     LAELARQRPDLLLLDVMLVALDGFQLCRIIRDHPEYGDLPIVILSGRETVADREAGLR
                     AGVSAYLTKPFRPEQLLEAVRTQLAAQPEVVA"
     gene            1516..2367
                     /locus_tag="VFV93_05475"
     CDS             1516..2367
                     /locus_tag="VFV93_05475"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5164826.1"
                     /translation="MQDKTSPATAFRDLTDETRAIWDAKADFWDDRMGEGNPFQQTLI
                     GPATERLLALREGERVLEIACGNGQMARRMATLGATVVATDFSRRFVELATARTEAAG
                     LSDRVTFQQVDATDEAALLAFGEGGYDAVVCNMALMDMTDVDPLFRAVARLLAPGGRF
                     VFTIPHPCFNSNAVRFTLEEEDRGGELVETAAVKVDDYLNIPPGKGAGMPGEPAPHWY
                     FHRPLSTLLPLAFATGLVMDGIEEPAFPPGTPSTRALSWFRFSNIPPVLAVRMRVLPR
                     ASSPLAM"
     gene            2653..3903
                     /locus_tag="VFV93_05480"
     CDS             2653..3903
                     /locus_tag="VFV93_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5164827.1"
                     /translation="MEDIAIVAALIAVGVLASEWLARHSVSLPIIFLLGGWILGQHVL
                     SWVDLSLDTEVVKELTELTLALLLFADASTLRRRRVQKEAGLEARLLAIGLLLTIALG
                     GLLAYLLFPDDDLGIVLLIGAALAPTDAALGLPIFTNRNIPLRIRQSLNVESGLNDGI
                     VTPFVTLFIALAVSEAPTAEGGWLVDAASELLLGVTVGVVVGVVGGVLIRQALSRNFT
                     SPETAQIAFFALSITAFFGSRELEGNGFIAAFVAGLFCGSMMKQWATEAAEFTETAGT
                     GASLFVWAVFGASLVPLALDEALSWQVVVFAILALTVMRMVPVAIAMIGTGFRRDTIA
                     IMGWFGPRGLASVVFLVTAIVALEDDGLPVTLIGAMTWTIGLSVILHGISAGPLARWY
                     GNRIERANDEIPERTTLDSNVLVT"
     gene            complement(3942..5690)
                     /locus_tag="VFV93_05485"
     CDS             complement(3942..5690)
                     /locus_tag="VFV93_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5164828.1"
                     /translation="MVQSRTARVKKYFTILDWLPGYKTEWLRPDIIAALSVWSLLVPQ
                     GIAYSSIAGVPAQYGLYAALGALLGYAVFGSSGQVCTGPSAAIAAVSASVVAQWADSG
                     SSEWIAYTSTLAVFAGLVYVVLGVLRMGWISHFLSGAVLAGFVFGFGFGLIVDQTPKI
                     LGIPKESGSYFQVLVGVVKDVPDTSMLTLTVGVLSILMLLLFRRFLPALPRTITVVVI
                     GILVSNVLNLDDHGVSVVGDIPTGLPSLVRPELPPVDLISVVLGSLAIIFIGYSESLA
                     TAKEAGRKHGYEIDASQELTAQGMANVGSGLLGGYSVEGSLSKTTVADMAGQKTQLAS
                     LLTAGLILLTILLLTGFFTSLPDAVLGAVVIDAGISLIKFREYRHYRLSNRDFAAFIA
                     TALVVFFIGVLAGVVVGVAIALLLLIASASNSPTRQMAFDPEDRAYVYVEHHPDAELI
                     PGIVIVGIHGPLFFADAENFRTSVEEIVATANPHTVVIDFGAVVMMDMDGVRALEMVV
                     EALRQKQIDVLLVSVHHDNMKLMRNLGTLKEVGGDNVFHTIRAAVASAQERTSPPVGS
                     TSGSQGPAQNRRTDAS"
BASE COUNT         1111 a         1943 c         1935 g         1091 t
ORIGIN      
        1 ggcgcctgct cggcgctggg ccggccaatc acgatctggg catcggccag caacccgtag
       61 acaccggcca gcgccaccga gaggtgctca agcgtcggcg cgccaagctc ggcgacgctg
      121 atctcatatt ccagcgtcga gaaggcttcg atcagcagcg actcgacgat cagcgcgtcg
      181 cgtccctcac tgccggcgtc ggcaggctgc gcgatccgtg cggcatccag gatcgaggcc
      241 aggtcacgga ccggcgcggg gtggaccgca atcgacagat cgtcgaacgc ggccaggaag
      301 gtgatgtcgg ccagatctgc cttcaaaatc cgctcgtcgg tgctgtagga cgccagcggg
      361 gcgccatccg caaccagaac gacgacggca tacccttcgg tcaccgtcag ccgcagcgcg
      421 ccgctgaagc gctcgcgccg cagccgggcg aggatcgtgt cgagcgagcc gccggccagt
      481 gtcaacgggc tgaggctgga cggcggcgag aagagaccgg ccatgcagcg ggccagcccc
      541 tcatcgaccg gatggctgac gacgaaccat tcgccctgct cgccatcgag cagatcgtgc
      601 agcgcctgcg cgccaaccgc gtcgccgccg cgcgtctggg cggtctcgac cagcctgccg
      661 cccgcgtaga gcagcacgac ggtctggccg gcgctggaca gcagcgcgta gccatcgcgc
      721 tcctcgcgga tcatgtcggc cagccagccg ctcaatgcag gcgcggcgac cggctcggca
      781 cgcctggcag gcggctccgg caggacgacc tgttcggtcg tcagaaatgt gatatcgctc
      841 tcagacgtgg caagcatggc cgacatcagg caaccacctc cggctgcgcg gccagttgcg
      901 tgcggacggc ctccagcagt tgctccggcc ggaatggttt ggtcaggtag gcgctgacgc
      961 cggcccgcag gccggcctca cggtcggcga ccgtctcgcg gccggagagg atcacgatcg
     1021 gcaagtcgcc gtactcagga tggtcgcgga tgatgcggca gagctggaag ccatccagcg
     1081 cgaccagcat gacgtccagc agtagcagat cgggccgctg gcgggccagt tcggccaggg
     1141 ctttcaaccc cgaatcggcc gtgacgacgc tgtagccttc gcggcgcagc gtcaactcga
     1201 ccagcttgcg aatgctcgcg ctgtcgtcga cggccaggac ggtgatgtcg cctactgctc
     1261 ggtcttgcat ggattggaca ctcctgcgtg attgaacttc tatctatagt aggcgtgtga
     1321 cgcccggtca tcactgcgtc gtcggtcccc aatttcggct ggaaaccggg gcgatactcc
     1381 tagtacggga gggctgagac aatcttcccg aaaccccctc ccctgaaaag ggagggggtt
     1441 tcgggaagat tgctacgcct cacattcggt atcatcctca cccgcatccg atagcaagcg
     1501 agggagagcg cgcgcatgca ggacaagacg tcgcccgcca cggcatttcg tgacctgacc
     1561 gacgagacgc gcgcgatctg ggacgccaaa gctgacttct gggacgaccg gatgggcgag
     1621 ggtaacccgt tccagcaaac gctgatcggc ccggcgaccg agcggctact cgcgctccgc
     1681 gagggcgagc gcgtgctgga gatcgcctgc ggcaacggcc agatggcgcg ccggatggcg
     1741 acactcggcg ccaccgtcgt cgcgaccgac ttcagccggc gcttcgtcga gctggcgacg
     1801 gcccggaccg aggcggccgg actcagcgac cgggtcacgt tccagcaggt cgacgcgacc
     1861 gacgaggcgg cgttgctggc gttcggcgag ggcggctacg acgctgtcgt ctgcaacatg
     1921 gcgctgatgg acatgaccga tgtcgatccg ctcttccggg ccgtcgcccg gctgcttgcg
     1981 ccggggggcc gcttcgtctt cacgatccca catccctgtt tcaactcgaa cgcggtccgc
     2041 ttcacgctcg aagaggagga ccgtggcggt gagctcgtcg agacggcggc tgtcaaggtc
     2101 gacgattacc tcaacatccc gcccggcaag ggcgccggga tgcccggcga accggctccg
     2161 cactggtact tccaccggcc gctctcgacc ctgctgccgc tcgccttcgc gaccggtctc
     2221 gtcatggacg gcatcgagga gccagccttc ccacccggca cgccgagcac gcgcgccctg
     2281 agctggttcc ggttcagcaa catcccgccg gtcctggccg tccgaatgcg cgttctgcca
     2341 cgagcgtcca gcccactcgc aatgtagttg cgagtcggtg actcgcggct acgtattggg
     2401 tgggctgttg cgcggaatgg ctccatcgcc atgtccaggc aggtagctcc tactcccctt
     2461 tgcgccccaa cggttgactg aacgcaggcg gcaaactggg acaattgcca tgtctcgggt
     2521 cgattgagcg ttcgccgaca cgcgccacga cctggcccgc ccggctccct ccttcgacca
     2581 acccaacacg tggcaaccct ttcgacgatt gctatggaac ggtgatgccc gttcatatcg
     2641 gcaggaactt tcgtggaaga catcgcgatc gttgcagccc tcatcgctgt cggtgttctc
     2701 gcgtcggaat ggttggcgcg tcattcagtg tcgctgccga tcatcttcct gctgggtggc
     2761 tggatcctcg gccagcacgt cctatcctgg gtggatctgt cacttgacac cgaggtcgtc
     2821 aaagagctca cggaactgac gctcgcgctc ctgctctttg ccgatgcctc gacgttacgc
     2881 aggcgtcgcg tccagaagga agctggtctg gaggctcgcc tgctggcgat tggactcctg
     2941 ctgacgatcg cgctcggtgg gctgctggcg tatctgctct tccctgacga cgatctgggg
     3001 atcgtgctgc tcatcggtgc ggcgctggct ccaaccgacg ccgcacttgg cctgccgatc
     3061 ttcacgaacc gcaacattcc actccgtatc cgccaatccc tgaacgtcga gagtggcctg
     3121 aacgacggga tcgtcacccc gtttgtcacg ctgttcattg cgctggcggt ctcggaagcc
     3181 ccgaccgcag agggcggctg gcttgtcgat gcagcgtccg agttgttgct cggagtcacc
     3241 gttggcgttg tggtcggtgt tgtcgggggc gtgctgatcc gccaggcgct gtcgcggaac
     3301 ttcaccagcc cggagacagc ccagatcgcg ttctttgcac tgagtatcac ggcgttcttc
     3361 ggctcacgcg agttggaagg aaacggcttc atcgccgcct tcgtggctgg tttgttctgc
     3421 gggtcgatga tgaagcaatg ggcgacggag gcggccgagt tcaccgagac ggccgggacg
     3481 ggcgcctcgc tgttcgtctg ggccgtcttc ggggcgtcgc tcgtgccgct ggcgctcgat
     3541 gaggcactct catggcaggt ggtcgtgttc gccatcctcg cgctgacggt catgcgaatg
     3601 gtcccggtcg cgatcgcgat gatcggaacc ggattccgcc gtgacacgat cgcgatcatg
     3661 ggctggtttg gtccgcgagg tctggcgtcg gtcgtctttc tcgtaactgc catcgtcgcg
     3721 cttgaagacg atgggctgcc ggtcacgctg atcggcgcga tgacatggac gatcggcctg
     3781 agcgtgatcc tgcacggcat ctcggccggc ccactggcca ggtggtatgg aaaccggatt
     3841 gagcgcgcga atgacgagat ccccgaacgg acgacgctgg attcgaacgt cctggtcacc
     3901 tagagcggcg cgcagcaacg ttttcccatt gtgcagatcg gtcatgacgc gtcggtccgc
     3961 cggttctggg ccggcccctg cgagcccgag gttgaaccga ccggcggact ggtccgctcc
     4021 tgtgcgctcg ccaccgcagc acgtatcgtg tgaaagacgt tgtcgcctcc aacctctttc
     4081 aacgttccca gattgcgcat gagtttcata ttgtcgtggt ggacactcac gagcaggaca
     4141 tcgatctgct tctggcgcaa tgcctcgaca accatctcaa gcgctctgac gccatccata
     4201 tccatcatca cgactgcacc aaagtcgatc acaacagtat gggggttggc cgtggccacg
     4261 atctcttcta ccgacgtgcg aaagttctcc gcgtccgcga agaacagcgg accatggatc
     4321 ccgacgatga caataccggg gatcaactca gcgtcggggt gatgttcgac gtacacgtag
     4381 gcgcgatcct cagggtcaaa cgccatctgg cgggtcggag aattcgaggc agaggcgatc
     4441 aggagaagga gagcgatggc gacaccaacc acgactcccg caagcacgcc gatgaagaag
     4501 accaccagcg cggttgcgat gaaggctgca aagtcgcggt tgctgagtcg atagtgccgg
     4561 tactcacgga atttgatcag gctgattccc gcgtcgataa cgactgcgcc gagcacggca
     4621 tccggcagtg acgtgaagaa accagtcaac agcaggatcg tgagcaggat caagcctgcc
     4681 gtgaggagag acgcgagttg tgtcttctgg cctgccatat cggcgacggt cgtcttggag
     4741 aggcttccct cgacggagta tccgcccagc aatccggaac cgacattcgc catcccctgt
     4801 gcggtcagct cctgactcgc gtcgatctcg tagccgtgct ttcttcctgc ctcctttgcc
     4861 gtagccagag actcgctgta cccgatgaag atgatcgcca atgatcccag gacgactgag
     4921 atcaggtcga ccggcggcag ctcaggccgg accagcgaag gcagaccggt tggaatgtcg
     4981 ccaacgacgg agaccccgtg atcatccagg ttcagcacgt ttgaaaccag gatcccaatg
     5041 acgacaacag tgatggtacg cggcaacgcc ggcaagaatc ggcgaaacag cagcaacatc
     5101 aggatgctca aaacgccgac cgtcagcgtc aacatgctgg tatcaggtac gtctttgacg
     5161 accccgacca gcacctgaaa atagctcccg ctctccttgg gtatgccgag aatcttcggc
     5221 gtttgatcga cgatgagtcc gaagccgaag ccgaacacga atccggccag gaccgcaccg
     5281 gagaggaagt gtgagatcca gcccatccga agcacgccaa gcacgacgta gaccagcccg
     5341 gcgaataccg ccagcgtgct cgtataggcg atccattcgc tcgaacccga atcggcccat
     5401 tgggccacga cggatgcgga cacggcggcg atcgccgcac tggggccggt gcaaacttga
     5461 cctgatgaac cgaagactgc atagccgagc agcgcgccca gggcggcata caggccgtac
     5521 tgggccggca cgccggcaat ggatgagtag gcgatgccct gtggcacgag cagtgaccac
     5581 actgacagcg cggcaatgat gtcaggtcgc agccactcgg ttttgtaccc tggtagccag
     5641 tcaagaatgg tgaagtattt cttgacgcgc gcagttcgcg attggaccat gtcggcagat
     5701 cctttgtttg atctgacgat cgatcgaggt gccgctgagg gcccatctcg atgagcagag
     5761 gcctatccgt gtcctacggt cccgagcggg cgagcatccc tcccgcgtaa cggtcttcgc
     5821 cgtgttgcag tcgcgcaggg cacggttgtg ccagcgcggc gggtgctgag cgatacccat
     5881 tcgtacacgg ccggctcaga acgataggcg acggctctca tcataagatc ggatattgcc
     5941 cagtggcatg ctacgggagg gtgtagttcc acggtgcagg cgcaaggcgg cccacatgcc
     6001 gggccgggtt tcaccagatt ccatccacga aaaccgcctc atctgctccc ggccagggcg
     6061 gcatcgtcag catgatgaac
//
LOCUS       DASUGJ010000250         3026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_314633, whole genome shotgun sequence.
ACCESSION   DASUGJ010000250 DASUGJ010000000
VERSION     DASUGJ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314633"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..802)
                     /gene="rplA"
                     /locus_tag="VFV93_05490"
     CDS             complement(77..802)
                     /gene="rplA"
                     /locus_tag="VFV93_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX5164829.1"
                     /translation="MPKHGKKYEAALAQVEPDKLYTPEEAVALLKKIKFSNFDETVEV
                     HVRLGIDPRQADQAVRSTVVLPAGTGKEVRVLVFAVGDAARIAQESGADYVGSDDLVK
                     QINDGWLEFDATVAMADQMGKVGGLGRILGRRGLMPNPRSGTVVRTPEDLPDVLNELK
                     GGRVEFRNDRTGLLHVAIGKVSFDEQRILQNLNALVDAVQRNKPSAQKGVYLRSITLT
                     STMTPGIPLDVTAAVAASSQAAA"
     gene            complement(866..1288)
                     /gene="rplK"
                     /locus_tag="VFV93_05495"
     CDS             complement(866..1288)
                     /gene="rplK"
                     /locus_tag="VFV93_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX5164830.1"
                     /translation="MAKKIRAYVKLQIPAGKATPAPPIGPALGQHGVAIMNFCKEYNE
                     RTAGQAGQVIPVLITVYEDRSFTFVTKTPPAADLLRRAAGIEKGAGNVKEMAGQVTRA
                     QVREIAELKMRDLNAIDVEGAMKQVEGTARSMGIEVAG"
     gene            complement(1394..2014)
                     /gene="nusG"
                     /locus_tag="VFV93_05500"
     CDS             complement(1394..2014)
                     /gene="nusG"
                     /locus_tag="VFV93_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX5164831.1"
                     /translation="MTRSIRERLASQREGYEESQGAPEGEQAHWYVIHTYSGYENKVR
                     QNLMHRVETMDVADRIFEVIVPTQDEIEIKNSQRHTVQRKVFPGYVLVKMIMDNETWG
                     VVRNTPGVTSFVGAGMVSGATQKPNPLTEDEVQSIMKGMTAEAPKVKVSLSMGDAVRI
                     MDGPFQDFRGVIDEINQEKGKIRVLVSFFGREVPVELDFLQVEREA"
     gene            complement(2070..2315)
                     /gene="secE"
                     /locus_tag="VFV93_05505"
     CDS             complement(2070..2315)
                     /gene="secE"
                     /locus_tag="VFV93_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX5164832.1"
                     /translation="MSTRTSRTTSKTSERPGNPGNFGKFQNLVRDTYSEIKKVTWPDQ
                     ETTRNLTILVILMSAALGILLGLVDYGFRQLWDWIPA"
     gene            complement(2521..2593)
                     /locus_tag="VFV93_05510"
     tRNA            complement(2521..2593)
                     /locus_tag="VFV93_05510"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2558..2560),aa:Trp,seq:cca)
     gene            complement(2610..2780)
                     /gene="rpmG"
                     /locus_tag="VFV93_05515"
     CDS             complement(2610..2780)
                     /gene="rpmG"
                     /locus_tag="VFV93_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX5164833.1"
                     /translation="MAKKSKADRIVITLECTECRERNYTSEKNRRNDSARLELRKFCP
                     RCRCHRSHRETR"
     gene            complement(2861..>3026)
                     /gene="tuf"
                     /locus_tag="VFV93_05520"
     CDS             complement(2861..>3026)
                     /gene="tuf"
                     /locus_tag="VFV93_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452162.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX5164834.1"
                     /translation="AIQLPEGVEMVMPGDNIQMQVELIQPVAIEEGLRFAIREGGRTV
                     GAGVVTSINK"
BASE COUNT          517 a         1005 c          871 g          633 t
ORIGIN      
        1 ggccggatga ttaagcaacg tgacgttgcc ccggctgtct tcggcgcgtc gtgattcgtc
       61 tgttggtccc gcgatgctag gcggctgcct ggctcgacgc cgcgacggcc gcggtgacat
      121 ccagcgggat accgggcgtc attgtactgg tcaatgtaat cgaccgcaaa tagacaccct
      181 tctgcgcgct cggcttgtta cgttggacgg catcgacgag cgcgttcagg ttctgcagaa
      241 tccgctgctc atcgaatgac accttgccga tcgcgacgtg tagcaggcca gtccggtcgt
      301 tacggaactc gacgcggccc cccttgagct cattcagcac gtcaggcagg tcttccggcg
      361 tccggacgac ggtgccggag cgcgggttcg gcatcagacc acggcgtccg agaatacggc
      421 caagaccacc gactttgccc atctgatcgg ccatcgcgac ggtcgcgtcg aactcgagcc
      481 agccgtcgtt gatctgcttg accaggtcgt cactcccgac gtaatcggcg ccggactcct
      541 gcgcgatccg ggcggcatcg ccgacggcga agaccaggac gcggacttcc ttgccggtgc
      601 cggccggcag gacaaccgtc gagcgcacag cctgatcggc ctgacgggga tcgatcccga
      661 gccggacatg gacctcgacc gtctcatcga aattggagaa cttgatcttc ttgaggagcg
      721 cgacagcttc ctctggggtg tagagcttat ccggctctac ctgcgcaagc gcagcctcgt
      781 acttcttgcc gtgcttcggc atggggctac ctcctgtggt gcgagcgggg cgcctgcccc
      841 tcccacgcga ctactctggc cggtactagc cggcgacctc gatgcccatc gagcgggcgg
      901 tgccttcgac ctgcttcatc gcgccttcga cgtcgatggc gttcagatca cgcatcttca
      961 gttcggcgat ctcacgcacc tgagcacgtg tgacctggcc ggccatctcc ttgacgttgc
     1021 cggcgccctt ctcgatcccg gccgcgcggc gtagcaggtc ggcagcaggc ggggtcttgg
     1081 tcacgaacgt gaacgatcgg tcctcataga cggtgatgag aaccggaatg acctgtccag
     1141 cctggccggc ggtgcgctcg ttgtactcct tgcagaagtt catgatcgcg acaccatgct
     1201 gaccgagcgc cggaccgatc ggcggagccg gtgtggcttt gccggccgga atctgtagct
     1261 tgacgtatgc cctgatcttc ttggccaacc ctgacgaaac tcccttcccg gacccggtcc
     1321 cagttcccag gtcgttgcgc gtatccagac atgacgacgc cggcagccat ctgccggcga
     1381 ccgatcgggc gaattatgcc tctcgctcga cctgcaggaa atcgagctcg accggcacct
     1441 cgcgtccgaa gaatgagacg agcaccctga tcttgccctt ttcctggttg atctcatcga
     1501 tgacgccgcg gaagtcctgg aacggaccat ccatgatgcg aaccgcgtca cccatcgaca
     1561 gcgacacctt gaccttcggc gcctcggccg tcatgccctt catgatcgac tgaacctcgt
     1621 cctcggtcag cgggtttggc ttctgcgttg cgccggacac catcccagcg ccgacgaagc
     1681 tggtaacgcc cggcgtgttg cgaacgacgc cccaggtctc gttgtccatg atcatcttga
     1741 ccagcacgta tccgggaaag accttgcgtt ggacagtgtg ccgctggctg ttcttgatct
     1801 cgatctcatc ctgcgtcggg acgatgactt cgaagatccg atcggcgaca tccatcgtct
     1861 cgacgcggtg catcaggttc tgacgcacct tgttctcgta gcccgagtac gtgtggatga
     1921 cgtaccagtg tgcctgctcg ccttccggcg cgccctgact ctcttcatat ccttcgcgct
     1981 ggctcgccag ccgctctcgg attgacctgg tcaccgttcc tcgactctcc aatgacatcg
     2041 accgcctgcc tgatggcgac ggcgaccgac taggccggaa tccagtccca aagctggcgg
     2101 aaaccgtagt ccaccagacc gagcaggata ccgagcgccg cagacatcag gatcacgagg
     2161 atcgtcagat tgcgggtcgt ctcctggtcc ggccaggtca ccttcttgat ttcgctatag
     2221 gtatcccgga cgaggttctg aaactttccg aagttcccag gattcccggg ccgctccgat
     2281 gttttgctcg tcgtcctgga agtcctggtc gacatctcgc cttccttgcc cgtcctgtga
     2341 acaatgcccg acagagtccg gtcccagcgc aggtcgtcgt cggctcagac ccggcctgtc
     2401 gctctctttg atatcaccac gttcgtgaca gatcgtcaac acaaagacgg ggcgtgccag
     2461 ttatgctccg ccccacgcat gcgcttcatc tgatcgtctc ccttccggtg agggaagggg
     2521 cagggccggc aggaatcgaa cccgcaacct gcggttttgg agaccgctgc tctaccaatt
     2581 gagctacgac cctatgaacc agcgaatgct taccgcgtct cccgatggct gcggtggcaa
     2641 cgacagcgtg ggcagaactt ccgcaactca agccgggctg aatcgtttcg acgattcttt
     2701 tctgacgtgt aattccgctc gcggcactcg gtgcattcga gcgtaatcac gatccggtca
     2761 gccttgctct tcttcgccat tatacatcat ctcgctttcg gggtgggagc ggctggactg
     2821 ttccagccgc ctcacccaca acgtctcgcc tggctaaccg ttacttgttg atcgaggtga
     2881 cgacaccagc gccgacggtc cggccacctt cgcgaatggc gaagcgcagc ccctcctcga
     2941 tcgccaccgg ctggatcagc tccacctgca tctggatgtt gtcgcccggc atcaccatct
     3001 cgaccccctc gggcaactgg atggcc
//
LOCUS       DASUGJ010000251         6298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1147962, whole genome shotgun sequence.
ACCESSION   DASUGJ010000251 DASUGJ010000000
VERSION     DASUGJ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147962"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFV93_05525"
     CDS             complement(<1..305)
                     /locus_tag="VFV93_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5164835.1"
                     /translation="MDNSRPIPAAPFDSVAAGYDDAFTSHQLGHWLREMVWQRMAASF
                     APGQHVLELGCGTGEDALWLAQRGVHVTATDASPAMLAETRRKVERAGLGEFVTVAG"
     gene            complement(295..1695)
                     /locus_tag="VFV93_05530"
     CDS             complement(295..1695)
                     /locus_tag="VFV93_05530"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5164836.1"
                     /translation="MDILLTHGYCMYEDPHEMEVRKPYPPLGILYISAHLKAHGFDTK
                     VFDMTFKSLDAFAEYVKRERPPVVGIYCNLMTRPRIIKMIKHCKEAGSLVVAGGPDSA
                     NWSEEYLERGADVVVIGEGELTLEELLPHLARYGPNNMEHILGIVYRDGDGNIVRTPE
                     RPYIDDLTAQPFPDRAAIDIPEYVRVWKDNHGMGSVSLITARGCPYTCKWCSHAVYGY
                     THRRRSPENVADELQLIIDTYNPDMVWYADDVFTIHRGWFLKYVEELKKRGIKIPFET
                     ISREDRLKEEIVAGLAEIGCFRIWVGAESGSQRILDSMKRKTNAEGVIEVTHRLQRHG
                     IEAGMFIMLGYDGEEMEDLEASVDLLKRSNPDVFLTTVAYPIKGTPYYKDVADRVIAL
                     KSWEEGSDRDFTVAGRHSRRFYEHATRWMVNEVKLHKQRQSPDRDPVALAKAAINVQR
                     GRAGMLLTQHEVESGQ"
     gene            complement(1725..3137)
                     /locus_tag="VFV93_05535"
     CDS             complement(1725..3137)
                     /locus_tag="VFV93_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5164837.1"
                     /translation="MTRQKVLLYNPRAVFHTMPLALVALGSCLDLERFDVRIIDGRLE
                     ADPLGAVLAEAGDALCLGVTVLTGAPIRDALEVTRGVKARFPNLTTIWGGWHPSLFPT
                     DTLAEPSVDITVQAQGEVTFSEIVEGLAAGELPSAVRGTSSRVDGVATQHPPRPMAKM
                     DSFPSHNYNLIPVERYFTLKGERQLDYISSTGCFFRCSFCADPFVYNRRWVGLAPERI
                     AEEADQLWQRYHFSDFAFQDETFFTYPKRVVEIAERFIENGSRFTWTATMRADQGHRL
                     PDDVYELCVRSGLRRVMIGVESGSQEMLDWMTKDVTIEEIIYSAEKCVQHGIGAIFPF
                     IVGFPDETDASVRASMAMIKRLRSMSPEFETPVFYFKPYPGSRITEAVVRNGYKLPDS
                     LDGWAEFDFIGSSGPWVTDDKHQLIERFKFYNRFAWGPERLPRRPLQLLARWRCAHDF
                     YQLPVEKALVERLKPLPQLS"
     gene            complement(3134..4705)
                     /locus_tag="VFV93_05540"
     CDS             complement(3134..4705)
                     /locus_tag="VFV93_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5164838.1"
                     /translation="MSEVLLGQSYYLRFDPKLWEAMQPYPPLGTLYAASYIRERGYDV
                     ALFDAMLAESEAEWEQALQREQPRFAVIFEDNFNYLSKMCLLRMREAAYTMIRMARET
                     GATVIAAGADASDNGESYLASGADYVLYGEGDATLAELLDHLTGRSTTPLADIQGLIY
                     RDPEQGLVQTARRPDLKDLDALPFPAWELVDIPRYQAIWREHHGYYSMNMVTTRGCPF
                     HCNWCAKPIWGQRYNARSPENVAQELKWLKERFQPDHLWFVDDIMGLKPGWLERFATR
                     VEELDAKVPFKSLNRVDLLLRGDAVNALKRAGAKTIWMGAESGSQKILDAMEKGTRVE
                     QIYEAASRLHAAGIEVGFFLQFGYPGETREDIEMTLQMVRDCKPDDVGMSVSYPLPGT
                     KFHDWVRDQLGVKQNWLDSNDLAMLYKGTYTTDFYRQLHRVLHKEFRARKNRESLTRT
                     LRRPKQVRPQHLRQVAAMTAQLASLPIERRKLNRLEHQPRTGSPQIQIPLVLDPAAAS
                     RPSAQPARPEGEQPR"
     gene            complement(4702..5637)
                     /locus_tag="VFV93_05545"
     CDS             complement(4702..5637)
                     /locus_tag="VFV93_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164839.1"
                     /translation="MQEADWISRDDRRVIDISTELEQAIVAAVAYSDIFDYPLSPAEI
                     HRYLIEQPASREAVEAALAAPCIAEGPLSVRDGYVTLAGREASVATRQARLPDSERLW
                     QAALGYGRVIAGLPFVRMVAITGELAMDNVQPGSDIDYFIVTQPGRVWLCRLLTIGVV
                     RVAALRGVTICPNYLLAEHALTLHERNLYAAHELAQMVPIAGHAIYQRLRAENGWIED
                     YLPNAGGPPRKLGVRPWLAPLRRLAERALAGRVGDRLERWERERKIRRLSRIAGENGE
                     ASFSPDWCKGHVSGHERRILAAFDDRRRVLEEGVR"
     gene            5845..>6298
                     /locus_tag="VFV93_05550"
     CDS             5845..>6298
                     /locus_tag="VFV93_05550"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5164840.1"
                     /translation="MRGLVMVLRVMSYNIQDGGRLRLSLIATIIRRQQPDVVALLEAT
                     NRLAVAALARGLGMRLVFGPANNPFHVAWLSKLPIQRSANHRLPGLAKTLLEIEVGWA
                     GRPLQLFATHLAAGDDRLHPAEEAPRIIERIRAVAPGPHLLVGDFNALH"
BASE COUNT          979 a         1965 c         2235 g         1119 t
ORIGIN      
        1 aaccccgcca ccgtcacgaa ctcccccagc cctgcccgtt cgacctttcg ccgcgtctcg
       61 gccagcatcg ccggcgacgc gtcggtcgcg gtgacgtgga cgccgcgttg ggcgagccag
      121 agtgcgtctt cgccggtgcc gcagccgagc tcgaggacgt gctggccggg ggcgaaggac
      181 gcggccatgc gttgccagac catctcgcgt agccagtggc cgagctggtg gctggtgaag
      241 gcgtcgtcgt agccggcggc gacgctgtcg aagggggcgg ccggaatggg ccggctattg
      301 tccactttcg acctcgtgct gggtcagcag catgccggcc cggccgcgct ggacgttgat
      361 ggcggctttg gccagtgcga cggggtcgcg gtcggggctc tggcgttgct tgtggagctt
      421 gacctcgttg accatccagc gggtcgcgtg ctcgtagaag cggcgcgagt ggcggccggc
      481 gacggtgaag tcgcggtcgg agccttcctc ccaggatttc agcgcgatga cccggtcggc
      541 gacgtccttg tagtagggcg tgcccttgat cgggtaggcg acggtcgtca ggaagacgtc
      601 ggggttggag cgcttgagca ggtcgacgga ggcctcaagg tcttccatct cctcaccgtc
      661 gtagccgagc atgatgaaca tgccggcctc gatgccgtgg cgctggaggc ggtgggtcac
      721 ttcgatgacg ccctccgcgt tcgtcttgcg cttcatcgag tcgaggatgc gctgcgagcc
      781 ggactcggcg ccgacccaga tccggaagca gccgatctcg gccagaccgg caacgatctc
      841 ctccttgagc cggtcctcgc gcgagatggt ctcgaacggg atcttgatgc cgcgcttctt
      901 cagctcctcg acgtacttga ggaaccagcc gcgatggatc gtgaagacat cgtcggcgta
      961 ccagaccatg tcggggttgt aggtgtcgat gatcagctgc agctcgtcgg ccacgttctc
     1021 cggcgagcgg cggcggtggg tgtaaccgta gacggcgtgg ctgcaccact tgcaggtgta
     1081 ggggcagccg cgggcggtga tgagcgagac ggagcccatc ccgtgattgt ctttccagac
     1141 gcgaacatac tcggggatgt caatcgcggc ccggtcgggg aagggctggg cggtcaggtc
     1201 gtcgatgtag gggcgttcgg gtgtccggac gatgttgccg tcgccgtcgc gatagacgat
     1261 gccgaggatg tgctccatgt tgttcgggcc gtagcgcgcc aggtgcggca gcagctcttc
     1321 cagcgtcagc tcgccctcgc cgatgacgac gacgtcggcg ccgcgctcca ggtactcctc
     1381 cgaccagttg gccgagtcgg gcccgccggc gacgacgagg ctgccggcct ccttgcagtg
     1441 cttgatcatc ttgatgatgc gcggtcgagt catcaggttg cagtagatgc cgacgaccgg
     1501 cgggcgctcg cgcttgacgt actccgcgaa ggcatcgagg ctcttgaagg tcatgtcgaa
     1561 gaccttcgtg tcgaagccgt gcgccttgag gtgggctgag atatagagga tgccgagcgg
     1621 tgggtagggc ttgcgcacct ccatctcgtg cgggtcttcg tacatgcagt agccgtgcgt
     1681 caatagaata tccattgatt gttccctccc agatccccgg cggcctacga gagctgcggc
     1741 agcggcttga gtcgttcgac gagcgccttc tcgaccggca actggtagaa gtcgtgggcg
     1801 cagcgccagc gggccagcaa ctggagcggg cggcgcggca accgttccgg accccaggcg
     1861 aagcggttgt agaacttgaa gcgctcgatc agctggtgct tgtcgtcggt cacccacgga
     1921 ccggacgagc cgatgaagtc gaactcggcc cagccgtcca gcgaatccgg cagcttgtag
     1981 ccgttgcgga cgacggcctc ggtgatgcgc gagccggggt agggcttgaa gtagaagacg
     2041 ggcgtctcga actccgggct catcgaacgc agccgcttga tcatcgccat cgaggcgcgg
     2101 acgctggcgt cggtctcgtc cgggaagccg acgatgaacg ggaagatcgc gccgatgccg
     2161 tgctggacgc acttctcggc cgagtagatg atctcctcga tcgtgacgtc cttcgtcatc
     2221 cagtcgagca tctcctgcga gccggactcg acgccgatca tcacccgccg caggccggag
     2281 cggacgcaga gctcgtagac gtcgtcgggc agccggtgtc cctggtcggc ccgcatcgtg
     2341 gctgtccagg tgaagcgcga gccgttctcg atgaagcgtt cggcaatctc gacgacccgc
     2401 ttcgggtagg tgaagaaggt ctcgtcctgg aaggcgaagt cgctgaagtg gtagcgctgc
     2461 cagagctggt cagcttcttc ggcgatccgt tccggcgcga ggccgaccca gcggcggttg
     2521 tagacgaacg ggtcggcgca gaaggagcag cggaagaagc agccggtcga ggagatgtag
     2581 tcgagctgcc gttcgccttt cagcgtgaag tagcgctcga ccgggatcag gttgtagttg
     2641 tgcgacggga agctgtccat cttcgccatc gggcgcggcg ggtgctgggt cgcgacgcca
     2701 tcgacgcggg aggatgtgcc gcgaacggcg gatggcagct caccggccgc gagcccctcg
     2761 acgatttcgc tgaaggtgac ctcgccctga gcttggacgg tgatgtcgac cgacggctcg
     2821 gccagcgtgt cggtcgggaa gagcgagggg tgccagccgc cccagatcgt cgtcaggttc
     2881 gggaagcgcg ccttgacgcc acgggtgact tcgagcgcgt cgcggatcgg cgcgccggtc
     2941 aggaccgtca cgccgaggca gagggcatca ccggcttcgg cgaggacggc ccccagcgga
     3001 tcagcttcga ggcggccgtc gatgatgcgg acatcgaagc gttccaggtc gaggcacgag
     3061 ccgagcgcga ccagcgccag cggcattgtg tggaagaccg ctcgtgggtt gtagagcaga
     3121 accttctggc gggtcatcgt ggttgctccc cttctggacg ggctggctgg gcgcttggcc
     3181 ggctggcggc ggccgggtcg agcaccaacg gtatctggat ctgcgggctg ccggttcgtg
     3241 gctgatgctc cagccggttg agcttgcggc gttcgatcgg caggctggcg agctgggccg
     3301 tcatcgcggc cacctggcgc aggtgctgcg ggcgaacctg cttcggccgg cgcagcgtcc
     3361 gggtcagtga ctcgcggttc ttgcgggcgc ggaactcctt gtgcagcacg cggtggagct
     3421 ggcggtagaa gtcggtcgtg taggtgcctt tgtagagcat cgccaggtcg ttcgagtcga
     3481 gccagttctg cttgacgccg agctggtcgc ggacccagtc gtggaacttc gtccctggca
     3541 acgggtaaga gactgacatg ccgacatcgt ccggcttgca gtcgcgcacc atctggagtg
     3601 tcatctcgat gtcctcgcgg gtctcgccgg ggtagccgaa ctggaggaag aagccgacct
     3661 cgatcccggc cgcatgcagc cggctggcgg cctcgtagat ctgctcgacg cgggtgccct
     3721 tctccattgc gtcgaggatc ttctgcgagc cggactcggc gcccatccag atcgtcttcg
     3781 ccccggcccg cttgagggcg ttgacggcgt cgccgcgcag cagcagatcg acgcggttga
     3841 gcgacttgaa cggcaccttg gcgtcaagct cctcaacgcg ggtggcgaaa cgttccagcc
     3901 agccgggctt gaggcccatg atgtcgtcga cgaaccagag gtggtcgggc tggaagcgtt
     3961 ccttgagcca cttgagctcc tgggcgacgt tctccggcga gcgggcgttg tagcgctggc
     4021 cccagatcgg cttcgcgcac cagttgcagt ggaacgggca gccacgggtc gtgaccatgt
     4081 tcattgagta gtagccgtgg tgctcgcgcc agatcgcctg gtagcggggg atatcgacca
     4141 gctcccaggc cgggaacggc agcgcgtcga gatccttgag gtcggggcgg cgggctgtct
     4201 ggaccagtcc ctgctcggga tcgcgataga tcagcccctg gatatcggcc agcggggtcg
     4261 tgctcctgcc ggtcaggtgg tcgagcagct cggccagcgt cgcgtcaccc tcgccgtaga
     4321 gcacgtagtc ggcgccggac gccaggtagg actcgccgtt gtcggaggca tcggctccgg
     4381 cggcgatgac ggtcgcgccc gtctcgcggg ccatccggat catcgtgtac gccgcctcgc
     4441 gcatgcgcag caggcacatc ttcgagaggt agttgaagtt gtcttcgaag atgacggcga
     4501 agcgcggctg ctcgcgctgg agcgcctgct cccactccgc ctccgactcg gccagcatcg
     4561 catcgaagag ggcgacgtcg tagccgcgct cgcggatgta gctggcggcg tagagggtcc
     4621 cgagcggcgg gtagggctgc atcgcctccc agagcttggg atcgaagcgt agatagtatg
     4681 actggccgag gagcacttcg ctcatcgcac cccctcctcc aacacgcgcc ggcggtcatc
     4741 gaacgcggcc aggatgcgcc gctcgtgacc ggagacgtgt cccttgcacc agtccggcga
     4801 gaacgacgct tccccgttct caccggcgat ccgcgatagg cggcgaatct tgcgctcccg
     4861 ctcccagcgt tcgagacggt cgccaacgcg gccagcgagc gcccgctcgg ccagccgccg
     4921 gagtggcgcc agccagggcc ggacaccgag cttgcggggc ggcccgcccg cattcggcag
     4981 gtagtcttcg atccagccgt tctccgcgcg gagccgctgg tagatggcgt gaccggcaat
     5041 cggcaccatc tgtgccagtt catgggcagc gtagaggttc cgctcgtgca acgtcaaggc
     5101 gtgttccgcc agcaggtagt tcgggcagat cgtgacaccg cgcagggcgg cgacccgaac
     5161 gacgccgatc gtcagcagcc ggcagagcca gacccgccct ggttgggtga cgatgaagta
     5221 gtcgatatcg gacccgggct ggacattgtc catcgccagc tcgccggtga tggcgaccat
     5281 tcgcacgaac ggcaggccgg cgatgacgcg gccatagccc agcgccgcct gccagagccg
     5341 ctcggaatcg ggcagccttg cctgacgggt agcgacgctc gcctcgcggc cggccagcgt
     5401 gacgtagcca tcgcgcacgc tgagagggcc ctcagcgatg catggcgcgg ccagggccgc
     5461 ctcgaccgct tcacggctgg cgggttgctc gatcaggtag cgatggattt cggcagggct
     5521 gagcggatag tcgaagatgt cggagtaggc gacagccgcg acgattgcct gttccagttc
     5581 agttgagatg tcgatcaccc gccgatcgtc ccgggatatc caatcggctt cctgcatctg
     5641 gcgcccccga acgaacgcgc cgcgcgactt ccccatagaa ggtgcgcctg acgccagtct
     5701 tccgcgacac tgtactcgat tcgcggcacg ttcgtcgttc tattcggtca cggcggcatc
     5761 gtgtaccttg aaggcaggta cgtactggcg tcagtttgcg gattgtgcgt ggaatgtgca
     5821 acctgtcctt gggtggtgat gacgttgaga gggctggtga tggtgctgcg ggtgatgagc
     5881 tacaacattc aggatggcgg gcgtttgcga ctatcgctga tcgcgaccat catccgccgc
     5941 cagcagccgg atgtcgtcgc gctgctggag gcaaccaacc ggctggcggt ggcggcgctg
     6001 gcgcgtgggc tggggatgcg gctggttttc ggcccggcca acaacccgtt tcacgtcgcc
     6061 tggctcagca agctgccgat ccagcgcagt gccaaccacc ggctgcccgg cctggccaag
     6121 acgctgctgg agatcgaggt cggctgggcc ggccggccgc tgcaactgtt cgcgacccac
     6181 ctggcggccg gcgacgaccg gctccacccg gccgaggagg cgccacggat catcgagcgg
     6241 attcgtgccg tcgcacccgg cccgcacctg ctggtgggcg atttcaacgc gctccacc
//
LOCUS       DASUGJ010000252         4320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_37576, whole genome shotgun sequence.
ACCESSION   DASUGJ010000252 DASUGJ010000000
VERSION     DASUGJ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37576"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2144
                     /locus_tag="VFV93_05555"
     CDS             <1..2144
                     /locus_tag="VFV93_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX5164841.1"
                     /translation="HTDERHSYAQHDHHHRQQMAAEHVAHGAHGAMIASAMAPDHAAH
                     AGHGAHGMHDMSDPAMATGMERDMRNRFLVALLLTIPIILYSPAAENLFGIDLPGPAN
                     WIMLVLTTPIVLWAGWIFIEGAYYSLKARALNMSVLIATGVLAAYGFSVGLTLFTNDE
                     TFFEAAAMLVTFVLFGHWMEMKSRRGTTDALRALFDLVPPTATVVRDGVEVEIPSSEV
                     VVGDIVRLRPGDKVPVDGEIVSGTTSIDESLVTGESVPVEKAAGDSVIGGSINRGGSV
                     TFRATRVGSETALAQIVALVERAQSSKAPGQRLADWAAQWLVLLAVGSGIVTFVVWYV
                     FGSETAITALTFAISAVVIACPDALGLATPTVVAVGTGIGARHNILIKDAATLENVSG
                     VDTVVFDKTGTLTEGRPGLTDLVPAPGIDANDLLRLAADAERGSEHPLGEAIVRAADE
                     RGLMTGEATHFEAISGEGLAATVDGREVLIGNARLMANHAVEIAPLSDEIDRLAGAGR
                     TPMIVALDGELAGIIAVADTVRESARVTVRKLHESGVEVVMLTGDNKRTAEAVARELG
                     IDRVMAEVLPADKANHVANLQAEGRKVAMVGDGVNDAPALATADIGIAIGAGTDVAVE
                     TADVVLMKSDPLDVTRAIALSKATVRKMKQNLAWASVYNVLATPIAAGILYPSWGIML
                     RPEWSALLMSVSSIIVAINAVLLKRAEAELV"
     gene            complement(2411..2707)
                     /locus_tag="VFV93_05560"
     CDS             complement(2411..2707)
                     /locus_tag="VFV93_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164842.1"
                     /translation="MKLGVAVSALAVVVAVGLIVLTGGDSTAPTDQQSGAAAPATWTA
                     GEFSGGPRLAVDRATYDQGAVGYGEAVHAAFQLRNVGDEPLTLGEMSVNTLEGC"
     gene            complement(2802..3227)
                     /locus_tag="VFV93_05565"
     CDS             complement(2802..3227)
                     /locus_tag="VFV93_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HEX5164843.1"
                     /translation="MRRWSRVVAIAIMVALSAGAIASAPADDPGPLHATMLASVGTLP
                     EPVHQADHASVAAYQAAIEHPSVLASVPCLCGCIQSLGHTSNLDCYIESSSRGMTIYS
                     THGLNCLICQRITEDALAGAARGMSPAQLRMLIEEKYGG"
     gene            complement(3449..3832)
                     /locus_tag="VFV93_05570"
     CDS             complement(3449..3832)
                     /locus_tag="VFV93_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164844.1"
                     /translation="MNLSTLRELRGMERFFVFLVPIAIPVAIFHFVSGRIGNGIMATG
                     AILLGSVVLLISIRTRGEAAEVVVDNDDDDDWFGWLEDEEPADVLDRPTDEPLPAPFQ
                     SALDEIFSKRIPGSGDDDSHIHQPR"
     gene            4114..>4320
                     /locus_tag="VFV93_05575"
     CDS             4114..>4320
                     /locus_tag="VFV93_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5164845.1"
                     /translation="MLRPFRFAVINETVLPANDWLAHARRVEELGYSTFLIRDHFVPD
                     FFGDQLAPFSALTAAAMVTTRLRVG"
BASE COUNT          730 a         1404 c         1413 g          773 t
ORIGIN      
        1 aacacaccga tgaacgacac agctacgccc agcacgatca ccatcaccgg cagcagatgg
       61 cagccgagca tgttgctcat ggagcgcatg gcgcgatgat cgcatcggcg atggcgccgg
      121 accatgccgc gcacgctgga catggcgcgc acggcatgca cgacatgagt gatccggcga
      181 tggcgaccgg gatggagcgg gatatgcgca accgcttcct cgttgcgctc ctgctgacga
      241 tcccgatcat cctctactca ccggcggcgg agaacctgtt tggaatcgac ctgccggggc
      301 cggccaactg gatcatgctg gtgctgacga caccgatcgt tctctgggcc ggctggatct
      361 tcatcgaggg cgcgtattac tcgctgaaag cccgcgcgct gaatatgagt gtgctgatcg
      421 cgaccggcgt cctggccgcc tacggcttct cagtcgggct gacgctcttc accaacgacg
      481 agacgttctt cgaagcggcc gcgatgctgg tgacgttcgt cctcttcggc cactggatgg
      541 agatgaagag ccggcgcggg acgaccgacg ccctgcgggc gctgtttgat ctcgtcccgc
      601 cgacggcgac cgtcgtccgc gacggcgtcg aggtcgagat cccctcgtcg gaggtcgtcg
      661 tcggtgacat cgtccggctg cggcccggcg acaaggtgcc ggtcgacggc gagatcgtca
      721 gcggtacgac cagcatcgac gagtcactgg tcaccggcga gagcgtgccg gtcgagaagg
      781 cggccggcga cagtgtcatc ggcggctcga ttaaccgggg cggcagcgtc accttccgcg
      841 ccacccgtgt cggcagcgag acggcgctgg cccagatcgt cgcgctcgtc gagcgggcgc
      901 agtcgagcaa ggcgcccggt cagcgactgg ccgactgggc ggctcagtgg cttgtcctgc
      961 tggcggtcgg ctcggggatc gtcaccttcg tcgtctggta cgtcttcggc agtgagacgg
     1021 ccatcacggc gctgacgttt gcgatctcag ctgtcgtgat cgcctgcccc gacgcgctcg
     1081 gtcttgcgac gccgacggtt gttgcggtcg gaaccgggat cggtgcgcgg cacaacatcc
     1141 tgatcaagga cgccgcgacg ctggagaatg tctcaggtgt cgatacggtc gtcttcgaca
     1201 agaccggcac gctgaccgaa ggtcgtccgg gactgaccga tcttgtgccg gcgccgggca
     1261 tcgatgccaa cgatctgctg cgcctcgcag ccgacgccga acggggcagc gagcacccgc
     1321 ttggcgaggc aatcgtccgt gccgccgatg agcgcgggct gatgaccggc gaggcgacgc
     1381 acttcgaggc gatttccggt gaagggctgg ccgcgaccgt cgatggtcgc gaggtcctga
     1441 tcggcaacgc ccggttgatg gccaaccacg cggtcgagat cgctccgttg agtgacgaga
     1501 tcgaccggct ggccggcgcc ggccggacgc cgatgatcgt cgcgctcgat ggcgaattgg
     1561 ccggcatcat cgccgtcgcc gacacggtcc gcgagagcgc gcgggtgacg gtccgcaagt
     1621 tgcacgagtc gggcgtcgag gttgtcatgc tgaccggcga caacaagcga acggccgagg
     1681 ctgttgcccg cgagctgggc atcgatcgtg tcatggctga ggtgttgccg gccgacaagg
     1741 ccaatcacgt cgcaaacctt caggctgagg gccgtaaggt agcgatggtc ggcgacggcg
     1801 tcaacgacgc gccggccctg gcgacggccg atatcgggat cgcgatcggc gccgggaccg
     1861 acgttgccgt cgagacggcg gatgtcgtcc tgatgaagag cgatccgctg gatgtcaccc
     1921 gcgccatcgc gctctcgaag gcgaccgtcc gcaagatgaa gcagaacctg gcctgggcca
     1981 gcgtctacaa cgtcctggcg accccgatcg cggccggcat cctctatccg agttggggca
     2041 tcatgctccg cccggagtgg tcggcgctgc tgatgagcgt ctcgtcgatc atcgttgcta
     2101 tcaatgcggt gttgttgaag cgggcggaag cggaactggt ctgatcggga ttcgggacac
     2161 gggattcgtg gaaggtttac gccgttcatt gctcccgaat cccgtgttcc gattcccgcc
     2221 gccgatgcta cggcttcgga tactcccctg cgacggtcag cgacaccgaa ggctggacac
     2281 cgtcattgct gtcgaggacg atctcgaact ggtgcgggcc gcccattccc tcgtgcatcg
     2341 tgaactcgta gtgcagcgtc gtctcttcgc ccggcttgag ggtcgtctga ccgacgaccg
     2401 gttcggctgg tcagcacccc tccagggtgt tgaccgacat ctcacccagc gtcagtggct
     2461 cgtcgccgac gttccgcaac tggaaggctg cgtgaactgc ctctccatac ccgaccgccc
     2521 cctgatcgta tgtcgcccgg tcgaccgcca gcctcggccc tccgctgaac tcgccggccg
     2581 tccaggtcgc cggggctgct gcccccgact gctggtcggt cggcgctgtc gaatcaccgc
     2641 ctgtcaggac gatgagcccg acagccacaa cgactgccag tgcggagacc gccactccaa
     2701 gcttcatcaa ccgtcgctgc tgccgccgtt tccgggagaa ttcccgccgt ccggattgat
     2761 gtccatgcgc catcttgcgc cttctcctgc cccctgcggc cctagccgcc gtacttctct
     2821 tcaatcaaca tgcggagttg ggcgggactc atgccccgcg ctgcgccggc cagcgcatcc
     2881 tcggtaatcc gttggcagat caggcagttc aacccgtggg tggagtagat cgtcatgcca
     2941 cggctgctcg actcgatgta gcagtccaga ttgctggtgt gcccgagcga ttgaatgcag
     3001 ccacagagac atggcaccga cgcgaggacc gacggatgct ctatcgccgc ctgataggcc
     3061 gcgacgctcg cgtgatctgc ctgatggaca ggctccggaa gtgttcccac cgaggcgagc
     3121 atcgtcgcat gcagcggccc agggtcgtcc gccggtgcag atgcgatcgc gccagcgctg
     3181 agcgcgacca tgatcgcgat tgcgacgact ctactccatc tacgcaccca gctacctccg
     3241 tcatcagcga cgcactgcct gcgataacga taggcggcag catctgacag acgtatgccg
     3301 gcccgcagcg gcggcgttac aagcgcgtgt caattgggag cgaacaccag gagaggctgg
     3361 gaggattgga gtaaccggga tatgggtagg tcgggcagag cgcatcccca gacgcaccct
     3421 gatcccgatt cccgaacctg tggcctgctt atcgcggctg gtggatgtga ctgtcgtcgt
     3481 cgccgctgcc ggggattcgc ttgctgaaga tctcgtcgag ggcggactgg aatggggcgg
     3541 gtagcggctc gtcagtgggc cggtcgagta cgtcggccgg ttcttcgtct tccagccagc
     3601 cgaaccagtc atcatcgtcg tcgttgtcga caacgacctc ggccgcctca ccacgcgtgc
     3661 ggatgctgat cagcagcacg accgagccga gcaggatcgc gccggtcgcc atgatcccgt
     3721 tgccgatcct gccggacacg aagtgaaaga tcgcgaccgg aatagcgatc ggcacgagga
     3781 aaacgaaaaa tcgttccatg ccacgcaact cgcgtagcgt gctgaggttc aactcagact
     3841 cccgcccagg ccccgagacc tgtgctgttc atgctgcgga taaccgagtc cgtgtgtgcg
     3901 tctggtcagt cgataagcac ccaccaccca cgtagcacga agggtaccgc gactttgact
     3961 cgccgtcgat gcccctggcc caaattggcc atctcggcca ctgcccagcg aacgccactg
     4021 ccggtttgag cgcaggttcc ggatagaacc ggcgggagtg atcagtcatg ctggttgccg
     4081 ggcctgtgtt gcccccaagc gaagggacca gagatgctac gaccgttccg attcgccgtc
     4141 atcaacgaga ctgtcctgcc ggccaatgac tggctggccc atgcccgccg cgtcgaggag
     4201 ctgggttact cgaccttcct gattcgcgat cacttcgtgc cggacttctt cggcgatcag
     4261 ctcgcgccgt tcagcgcact gacggccgcc gcgatggtga cgacccggct gcgggtcggg
//
LOCUS       DASUGJ010000253         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1983368, whole genome shotgun sequence.
ACCESSION   DASUGJ010000253 DASUGJ010000000
VERSION     DASUGJ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983368"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VFV93_05580"
     CDS             <1..841
                     /locus_tag="VFV93_05580"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX5164846.1"
                     /translation="EPRGHADMYGAILTPPVTAEADYGILFMHNEGYSTMCGHGIIAV
                     TTILVETGQVAATGSSAMIGYDSPAGFVRATATVAPGGRVIEVAFENVASFVHAEAVP
                     VETQFGALTVPVVFGGAFYALAPVPAGLEVVPENADALIAFGMAVKHAVEAQLDVVHP
                     LDPGLHGIYGTIITGVPSDGCDGRNVTIFADGEVDRSPCGTGTAARLAWLHAQGDLPS
                     GQPYVHESIIGSRFTGRVLRETKVGSDAAIVPEIAGSAHITGYHTFVVEPDDSIGAGF
                     LIR"
     gene            complement(850..1587)
                     /locus_tag="VFV93_05585"
     CDS             complement(850..1587)
                     /locus_tag="VFV93_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5164847.1"
                     /translation="MDIQRIGIIGAGVMGSQIAQVLATGGHTVHLFDISNGQLETAMQ
                     RIEHGRFGLRGGVERGKLTEQELHATLERIIPVTELEAVCADADLIIEAVPENIKLKI
                     RVFRRLDAACPDRTILTSNTAGLSIAALAGATDRPEQVLGWHWFQPCAVMKLAELVTH
                     DETSDAALDAVKACAERSDRVVVTVKDQQLTWGFVGNRINRAVRLEAARIVDEGLATR
                     EQVDTIMREGFRWPMGPFELQGGGSLS"
     gene            complement(1599..>2118)
                     /locus_tag="VFV93_05590"
     CDS             complement(1599..>2118)
                     /locus_tag="VFV93_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5164848.1"
                     /translation="ILDEAFNQLPTGEVGEIFMRPFNPGEPTYYYIGSAPATSTPDGF
                     VSVGDMGYVDDEGYLFLADRRVDLIISGGANIYPAEVEAPLSQHPEIVDVVVIGIPDD
                     DWGKRVHAIVHPRDLDNPPSAASLDEWMRRHLASYKIPKTYEFVAELPRDGAGKIRRA
                     ALVAERSDAARK"
BASE COUNT          321 a          671 c          746 g          380 t
ORIGIN      
        1 ggagccgcgc gggcacgccg atatgtacgg cgcgatcctg acgccgccgg tgacagccga
       61 ggcggactac gggatcctct tcatgcacaa cgagggctac tcgacgatgt gcggccacgg
      121 gatcatcgcc gtgacgacga ttctcgtcga gacgggccag gtggctgcga ccgggtcgag
      181 tgcgatgatc ggctacgact cgccggccgg cttcgtccgc gcgacggcga cggtcgcgcc
      241 cggaggacgg gtgatagagg tggccttcga gaacgtggcg tcgttcgtcc atgcggaggc
      301 ggtgccggtt gagacccaat tcggcgcgct gacagtgccg gtcgtcttcg gcggagcgtt
      361 ctacgcgctg gccccggtac cggccgggct ggaggtcgtg ccggagaacg cggacgcgct
      421 gatcgcgttc gggatggcgg tcaagcacgc cgtcgaggcg cagctggatg tggtccatcc
      481 gctcgatcca ggcttacacg gcatctacgg cacgatcatc accggtgtcc cgtccgacgg
      541 ctgcgatggc cgcaacgtga cgatcttcgc cgatggcgag gtcgatcgat cgccgtgcgg
      601 gaccggaacg gccgcccggc tggcctggct ccacgctcaa ggcgacctgc cgagtggtca
      661 accgtacgtg catgagagca tcatcggcag ccgcttcacc gggcgggtgc tgcgtgagac
      721 gaaggttggc agcgacgcgg ccatcgtgcc tgagatcgcc ggctcggcac atatcaccgg
      781 ctaccacacg ttcgtcgtcg agccggacga ttcgattggt gcagggttcc tgattcgcta
      841 gccggtgcgt tacgacagtg agccgccgcc ttgcagttcg aacggtccca tcggccagcg
      901 gaacccctcg cgcatgatcg tatcgacctg ctcacgagtc gcgagtccct cgtcgacgat
      961 ccgggcggct tcgagtcgaa cggctcggtt gatccggttg ccgacgaagc cccaggtcag
     1021 ctgctgatcc ttgacggtga cgaccacccg gtcgctccgt tcggcgcaag ccttgacggc
     1081 gtccagtgcg gcatcgctcg tttcgtcgtg ggtcaccagc tcggccagct tcatgaccgc
     1141 gcacggctgg aaccagtgcc atcccagcac ctgctccggc cggtccgttg cgcccgccag
     1201 cgctgcgatc gagaggccgg ccgtgttgga tgtcaggatc gtgcggtcgg gacaggcggc
     1261 atccagccgc cggaagacgc ggatcttgag cttgatgttc tcagggacgg cctcaatgat
     1321 cagatcggcg tcagcgcaga cggcctccag ttcggtaacg gggatgatgc gctccagggt
     1381 cgcgtgcagt tcctgctcgg tcagcttgcc gcgctcaacg ccgccgcgca ggccgaatcg
     1441 gccatgttcg atccgctgca tggcggtctc gagctggccg ttgctgatgt cgaagaggtg
     1501 gacggtgtga ccgccggttg cgaggacctg ggcgatctgg gagcccatca cgccggcgcc
     1561 gatgattccg atgcgctgga tgtccacggg tgttcctttc atttgcgtgc cgcgtccgag
     1621 cgctcggcga cgagggccgc ccggcggatc ttgccggcgc catcgcgtgg cagctcggcg
     1681 acgaactcgt acgtctttgg gatcttgtag gacgccaggt ggcggcgcat ccactcgtcg
     1741 aggctggcgg ccgacggtgg gttgtcgagg tcgcgcgggt ggacgatggc gtggacgcgc
     1801 ttgccccagt cgtcgtccgg gatgccgata accaccacat cgacgatctc cgggtgttgc
     1861 gagagcggtg cttcgacctc ggccgggtag atgttggcgc cgccgctgat gatgaggtcg
     1921 acgcgccggt ccgccaggaa caggtagccc tcatcgtcga cgtagcccat gtcaccgacc
     1981 gagacgaagc cgtccggcgt tgatgtcgcc ggcgctgagc caatgtagta gtacgtcggc
     2041 tcgccggggt tgaacgggcg catgaagatc tcaccgacct cgccggttgg cagctggttg
     2101 aacgcctcgt cgaggatc
//
LOCUS       DASUGJ010000254         6881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1149681, whole genome shotgun sequence.
ACCESSION   DASUGJ010000254 DASUGJ010000000
VERSION     DASUGJ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149681"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFV93_05595"
     CDS             complement(<1..322)
                     /locus_tag="VFV93_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164849.1"
                     /translation="MNDAPAIMTPAPPASPAWLRRLVMTSPALLAVISAPVLITVGLL
                     VPDLGLILLFGFLGVAGGVVSVLRPGIGLFLFALMMYSRASEVLTTSLGIPSIAPLFT
                     IWLLT"
     gene            complement(325..1008)
                     /locus_tag="VFV93_05600"
     CDS             complement(325..1008)
                     /locus_tag="VFV93_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX5164850.1"
                     /translation="MTWNDVNAIILRVLRRWWIVLGIVAIVVGATFWGLSSSLDRYQS
                     TTLMVVGPNVDMEPSEVLRVADLLGSNMVMSTYADVMSSPKVVASGMVEVDPARENWS
                     DFEVRVVQEPDSNVLRMIVEGPDAATTASLAAAVQSNGQEALAELFPIYSISPLDAGA
                     PQAYLISLPWVRTIGISVVIGLGLGVLLALWFDSLLAYRQSSLGVINRADRASSAAVE
                     SRTAAYTRR"
     gene            complement(1045..2358)
                     /locus_tag="VFV93_05605"
     CDS             complement(1045..2358)
                     /locus_tag="VFV93_05605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5164851.1"
                     /translation="MSAPAEQAATEAMDSQSVAYIVLRFPTLSETFIVREIHALRSRG
                     WQVAIYPLWRDTPEHVHPDVPELEPSVRWTHPLSFAALRATLGMLARRPSRYLSALGL
                     VLSELPRRPKRAVISLGLFPVIVWMAGDLERRGIRHVHAHFASYPTLAAHIIQRLTGI
                     SYSFTAHAFDLYVDPSHLANKVSGATFIVTISEYNRERLRPLAGGVTPVHVIHCGVRV
                     PATLPERWRLGREVVCVARLEEKKGQTYLIDACRLLHERGASLHCTIVGGGPEMNRLR
                     AQIAAAGLEDVVTLAGPQTSERVQQYLAGASVFALPSVVTRSGNAEGIPVALMEAMAA
                     GVPVISTSTTGIPELVIDGETGLLVPPRDAVALADAIERLLDDSALGDELRLAAFQKV
                     RDEFELDANAREIERHLAGAIQDQLPLHGWSPACDDWHTERTANH"
     gene            2446..3588
                     /locus_tag="VFV93_05610"
     CDS             2446..3588
                     /locus_tag="VFV93_05610"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5164852.1"
                     /translation="MRQRLLMLLIGCAGYAYVGYPILLLLMNRIRDKPVAKGPAQPTF
                     SIIIPAHNEAQILPRKVASILNQDYPPDRVEVLVASDGSTDDSASVVAGNDRVRFLDY
                     PRGGKAATLNRAAAEALHEIIIFTDANAILAPGTLHAIAANFADPQVGGVSANEQRDP
                     SVTASPAGLGERLYWEYDKWVKQAESRIGSIVSASGSLYAIRRELFRPISDPSATDDF
                     AISTQVVRGGYRLVFEPEAVTWEPPVAKDEIEFNRKVRIVTRGLRSVWGIRELLLPWR
                     GGFYSVQLWSHKIVRRLVGFLATGIFLTNLSLVRRPFWRLVLAGQVAFYGLATLGWLG
                     SGRRWARKPWFYLPYYFCLSNIAATLGIIQFLRQRRVTIWEPRRDS"
     gene            3578..4738
                     /locus_tag="VFV93_05615"
     CDS             3578..4738
                     /locus_tag="VFV93_05615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5164853.1"
                     /translation="MTASHDSGRRLRVLMVMPSYYPVVGGMEIQIARQIPYLREHGID
                     AAILTRRPAGSPDFERHEGVAIRRISVPGGPGLRSISFTALGTLDIVKHRHEIDLIHA
                     HSIMSPTTIAALSGTLLRKPRLVTLHQSYEPEHLLGKPFGKQRLDLFRRIIPRFISIS
                     SDIEQLLLAHDVPAARIVSIPNGIDTTTFKPAPVTERTELRRQLHLPEGVPIAVFVGR
                     LQPVKQVDVLLEAWSSLTSGRLVILGDGDQRASLEAQATRLNLTDRVEFRGMVPNAVD
                     FLRAADIFVLPSASEGLSVALLEAMASGLVPIATAIGGTTDLIRDGQTGLLVQSSDVA
                     GLNAALCHALADADWRREVSEAARTFVVANYDMRVITAQLAGVYRDVVDATG"
     gene            complement(4833..6026)
                     /locus_tag="VFV93_05620"
     CDS             complement(4833..6026)
                     /locus_tag="VFV93_05620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5164854.1"
                     /translation="MLEAMKDAISDSTQVTGCDPLRIAVALDGDPLATTAAGTKFGLF
                     IEALRSHREVHLVGVGDSTLRGAPRAVAVAAAWRPNRRRWKEHYRKNPLTFQLRSRQS
                     RRWVAGLQPQPELLFQVAAISRPQSRTNIPYALYLDFTFALTQREWPERAPMSRLELP
                     LWLRLETQTYHGAAVIFCRSRYVANSLFSDYGVPPDRVHVVGAGVNLTPPDLAAIPGR
                     DHPRVLFIGSDFKRKGGDIVLAAWRDVVRRIPEAELMLIGPVEGRLPPNARTNAGRWD
                     PATIVRELQRASLFAMPSRCETWGDVFLEAMAYGLPCIGSSNDAMPEIIQDGETGYIV
                     PPDDPAALADRICDLLLNPGRARAFGLAGRRRVEERYLWPDVIDQMLPVLASIPDNKP
                     RNPLA"
     gene            complement(6229..>6881)
                     /locus_tag="VFV93_05625"
     CDS             complement(6229..>6881)
                     /locus_tag="VFV93_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164855.1"
                     /translation="LKERMEAAGWETASVWTGGRPYVSRPLVRLVKRRLRAPVQRSDG
                     SFEARTAGSEASEAFGEYLSKSNAMFRKHWILRRGWTDVSLIEHLIEATLAVQPHLRA
                     GRAVICDRYVYKSVVNLAILLDLPLTELPGLLRHPAVRLAPKPDLYFLMDLPASVAAE
                     RKTDLPSLDYVARRVPVYRALAAYANMPVVDATQPPDAVACDIWEDVSRVLPARSS"
BASE COUNT         1204 a         2330 c         2239 g         1108 t
ORIGIN      
        1 cggtcagcag ccagatcgtg aacagcggcg cgatcgacgg gattcccagc gaggtcgtca
       61 ggacctctga ggcgcgtgag tacatcatca gtgcgaagag aaacaggccg atgccaggcc
      121 ggagcacgct gacgaccccg ccggcaaccc ccaggaagcc gaacagcagg atcagcccca
      181 ggtcgggcac cagcagaccg accgtaatca gcaccggcgc cgagataacc gccagcaacg
      241 ccgggctggt catcaccagt cgccgcaacc atgctggaga cgccggtggc gccggcgtca
      301 tgattgccgg ggcgtcgttc atcctcatcg ccgcgtatat gccgccgtcc gcgactcgac
      361 agcagcggaa gatgctcggt cagcgcgatt gatgacgccg aggctgctct gccggtaggc
      421 cagcagcgag tcgaaccaga gcgccagcag gacgccgagc ccaagcccga ttacgaccga
      481 gatcccgatc gtccggaccc acggcaggct gatcagatac gcctgcggcg cgccggcatc
      541 cagcggcgag atcgagtaga tcgggaagag ttcggccagc gcctcctggc cgttcgactg
      601 gaccgccgcc gccaggctgg ccgtcgtggc ggcatccggc ccctcgacga tcatccgcag
      661 cacgttcgaa tccggctcct gaacgacccg tacctcgaag tcggaccagt tctcgcgtgc
      721 cggatcgacc tcaaccattc cgctggcgac gaccttcggg ctggacatga catccgcgta
      781 ggtggacatg accatgttgc tgccgagcag gtcggccacc cgcagcactt ccgacggctc
      841 catgtcaacg ttgggaccga cgaccatcag cgtcgtcgac tggtagcggt cgaggctcga
      901 cgacaatccc cagaacgtgg cgccgacgac gatcgcgacg atcccgagga cgatccacca
      961 gcgtcgcagc acccgcagga tgatcgcgtt gacatcattc caggtcacgt tgcaaatcct
     1021 tgtcgtcagc accgtcccgg cgactcagtg gttcgccgtg cgttcggtgt gccaatcgtc
     1081 acatgccggg gaccatccgt gaagcggtag ctggtcctga atcgcgccag caagatgccg
     1141 ctcgatctcg cgagcattgg catccagctc gaactcatcg cgtacctttt gaaacgcggc
     1201 cagccgcagt tcgtcaccga gcgccgagtc gtccagcaag cgctcgatcg cgtctgccag
     1261 cgcaaccgcg tcgcggggcg ggaccagcaa ccccgtctcg ccgtcgatca ccagctccgg
     1321 gatgccggtc gtcgaggtcg agatgaccgg cacgccggcc gccatcgcct ccatcagcgc
     1381 gaccgggatg ccctcagcat tgcccgagcg ggttacgacg ctcggcagcg caaaaacgct
     1441 cgccccggcc agatactgct ggacacgctc cgacgtctgc ggccctgcca gcgtcaccac
     1501 atcctcaagg ccggcggctg cgatctgcgc ccggagccgg ttcatttcag gtccgccgcc
     1561 gacaatcgta cagtgcagcg aagccccacg ttcatgcaac aggcggcagg cgtcgatcag
     1621 gtacgtctgc cccttcttct cttccagccg cgcgacacag acgacctcgc gacccagccg
     1681 ccagcgctcc ggcaacgttg ctggaacgcg cacgccacaa tgaatgacat ggaccggcgt
     1741 gacacctcca gccagggggc gaagtcgctc acgattgtac tcggagatcg tgacgataaa
     1801 agttgcgcct gataccttat ttgccagatg cgaaggatcg acatacaggt cgaaggcgtg
     1861 ggcggtgaat gagtaggata tgcctgtcag ccgctggatg atgtgcgccg ccagcgtcgg
     1921 gtagctcgcg aaatgggcgt ggacatgccg gattccgcgc cgttcgaggt cgccggccat
     1981 ccagacgatg accggaaaga gccccagcga gataactgcc cgcttcggcc ggcgcggcaa
     2041 ctcgctgagg accagcccga gcgccgacag gtaccgactc ggccggcggg cgagcatccc
     2101 aagcgtcgcc cgcaacgccg cgaaggacag cgggtgcgtc cagcggacgg aaggctccag
     2161 ctccggcacg tcgggatgga catgctcggg cgtgtcgcgc cagagcggat agatggcgac
     2221 ttgccagccg cgcgagcgca gcgcgtggat ttcgcggacg atgaacgtct cggagagtgt
     2281 cgggaagcgc aggacaatgt acgctactga ttgcgagtcc attgcctctg ttgccgcctg
     2341 ttcagccggc gcgctcaagc ctgctccctt cgctgattcg caccgatcgt ccagcagcgg
     2401 gtccgcaccc ggccgccccg cccacgattc ccgaaacgag gtggcatgcg ccagcgactc
     2461 ctgatgctgc tgatcggctg cgccggctac gcgtacgtgg gctaccccat cctgctgctg
     2521 ctgatgaacc gcatccggga caaaccggtt gcgaaagggc ccgcccagcc aaccttctca
     2581 atcatcattc ctgcccataa tgaagcacag attctccccc gaaaggtagc cagcatcctg
     2641 aatcaggact atccgcctga tcgggtcgag gtgctggtcg cgtcggacgg ctcgaccgac
     2701 gacagcgcct cggtcgtcgc cggcaatgat cgcgtccgct tcctggacta cccccgtggc
     2761 ggcaaggcgg cgacgctgaa ccgggccgcc gccgaagcac tgcatgagat catcatcttc
     2821 accgacgcca acgcgatcct cgcccccgga acgctgcacg ccatcgccgc caacttcgcc
     2881 gatccgcagg tcggaggcgt cagcgccaac gagcagcgcg acccgagcgt gaccgcctcg
     2941 ccggccgggc ttggcgaacg gctctattgg gaatatgaca agtgggtcaa gcaggccgag
     3001 agccggatcg gctcgatcgt ctcggcctcc ggctcgctct acgccatccg ccgcgagctg
     3061 ttccggccga tcagcgaccc atcggcgacc gacgacttcg ccatctcaac ccaggtcgtc
     3121 cggggcggct accggctcgt cttcgagccg gaggccgtca cctgggagcc gccggtcgcc
     3181 aaggacgaga tcgagttcaa ccgcaaggtc cggatcgtca cgcgcggcct gcgcagcgtc
     3241 tggggcatcc gcgagctgct gctaccctgg cgcggcggct tctacagcgt ccagctctgg
     3301 tcgcacaaga tcgtccggcg cctggtcgga ttcctggcca ccggcatctt cctcacgaat
     3361 ctctcgctgg tgcgacggcc gttctggcga ctggtgctgg ccggtcaggt cgcgttctac
     3421 gggctggcga cgctgggctg gctgggcagc gggcgacgct gggcgcgcaa gccgtggttc
     3481 tacctgccct actacttctg cctctcgaac atcgccgcga cgctcggcat catccagttc
     3541 ctgcgccagc gcagggtgac gatctgggag ccccgccgtg acagctagcc acgacagcgg
     3601 gcggcggctg cgggtgctga tggtgatgcc ctcgtactac ccggtcgtcg gcggaatgga
     3661 gatccagatc gcgcgccaga ttccctacct gcgcgaacat ggcatcgacg ccgccatcct
     3721 cacccgccgc ccggccggct caccggactt cgagcggcac gaaggcgtcg ccatccgccg
     3781 catcagcgtg ccgggcggtc cgggccttcg atcaatctcg ttcacggcac tcggaacgct
     3841 cgatatcgtc aaacatcggc acgagatcga cctgatccac gcccacagca ttatgtcacc
     3901 aacgacgatc gccgccttgt cgggaacgtt gctacgtaag ccgcgtctcg ttacgttgca
     3961 ccagagctat gaacctgagc acctgctggg caagccgttt gggaagcaac gtctggatct
     4021 gttccgccgc atcatccccc gtttcatcag catcagcagc gacatcgagc aactgctgct
     4081 ggcccatgac gttccggccg ctcggatcgt ctcaattcca aatggcatcg acacgaccac
     4141 gttcaaacct gcaccggtca ccgagcgaac cgaacttcgc cgtcagttgc acctgcccga
     4201 aggcgtaccg atcgccgtct tcgtcgggcg gctccagccc gtcaagcagg tcgatgtgtt
     4261 gctggaagca tggtcgagtc tgacatccgg ccggctcgtc atcctcggcg acggcgatca
     4321 gcgcgccagc ctggaagccc aggcgacgcg gctgaacctc accgaccggg tcgaatttcg
     4381 cgggatggtt cccaacgccg ttgacttcct gcgggccgcc gacatcttcg tcctgccatc
     4441 cgccagcgag ggactctccg tcgccctcct cgaagcgatg gcatccggcc tcgtcccgat
     4501 cgcgaccgca attggcggca cgaccgacct gatccgggat ggccaaaccg gcctgctcgt
     4561 gcaatccagt gatgtcgcgg gcctcaacgc cgcgctctgt cacgcgctgg ccgacgcgga
     4621 ctggcggcgt gaggtatccg aagcggcacg gacgttcgtc gtcgccaact acgacatgcg
     4681 ggtgatcacg gcgcagctcg ctggtgtcta tcgtgacgtg gttgatgcta ccggctaacg
     4741 ggaggaaatc ctcgcgtagg ggcgatggcc gcagcctcgc ccttcgtcgg tcgctcgagc
     4801 gttgggcaag ggcgaggctg cggccatcgc ctctacgcga gggggtttcg cggtttgttg
     4861 tcggggatcg acgccagaac cggcaacatc tggtcaatca cgtccggcca gaggtagcgt
     4921 tcctcgacgc gccggcggcc cgccaggccg aaggcacgcg cgcggccggg attcagcagc
     4981 agatcgcaga tgcggtcggc cagagctgcg gggtcgtcgg gcgggacgat gtagccggtt
     5041 tcgccgtcct ggatgatctc gggcatcgcg tcgttggacg agccgatgca gggcaggccg
     5101 taggccatcg cttcgaggaa cacatctccc caggtctcgc agcgggaggg catcgcgaag
     5161 agcgaggcgc gctggagctc gcggacgatt gtcgccgggt cccagcggcc cgcgttggtt
     5221 cgggcattgg ggggcaatcg cccctcgact gggccgatca gcatcagttc cgcctcgggg
     5281 atacggcgga cgacgtcccg ccaggcggcg aggacgatgt cgccgccctt gcgcttgaag
     5341 tcgctgccga tgaacagcac acgcggatgg tcgcgacctg ggatcgccgc caggtcgggt
     5401 ggcgtcaggt tgacgccggc gccgacgacg tgaaccctgt cgggcgggac accgtagtcg
     5461 ctgaacagtg agttggcgac gtaccggctg cgacagaaga tgaccgccgc gccgtggtag
     5521 gtctgcgttt caagccgcag ccagaggggc aattccagcc gtgacatcgg cgcccgctcg
     5581 ggccactcgc gctgcgtgag cgcgaaggtg aagtcaagat agagcgcgta ggggatgttg
     5641 gttcgcgatt ggggacggga gatggccgcg acctggaaga ggagctccgg ctgcggttgc
     5701 aggccggcga cccagcggcg cgactggcgg ctgcgcagct ggaacgtcaa cgggttctta
     5761 cgatagtgct ctttccagcg ccgtcggttc ggtcgccagg cggctgcgac cgccaccgcg
     5821 cgcggagcgc cacgcagggt cgaatcgccg acgcccacca ggtggacttc acgatgcgac
     5881 ctgagcgcct cgatgaacag gccgaacttg gtcccggcag cagtcgtcgc cagtgggtcg
     5941 ccatccagcg cgacggcgat ccgcagcggg tcgcagccgg tcacctgcgt gctgtccgag
     6001 atcgcgtcct tcatcgcctc aagcacgctg cgtcgctcca ctgtgtcctg tcaatgtttt
     6061 cacccgtcgc taataaggct gccacgccac atcggctgct tcaaggcgtg cgtggatgaa
     6121 caattcgcct gagacgatgc accgaacccg ctgtcgtcct gagtccgcag ccgaaggacc
     6181 tccccaactg aatcggggtg aatgcggaga ggtccttcgg ctgcggcctc aggacgaccg
     6241 ggctggcagg acgcgggaga catcctccca gatatcgcag gcgacggcgt cgggcggctg
     6301 ggttgcatcg acgaccggca tgttggcgta ggcggccagt gcgcggtaga ccggcacgcg
     6361 gcgcgcgacg taatccagcg acggcagatc agtcttccgc tccgccgcga ccgaggcggg
     6421 caggtccatc aggaaataca ggtccggctt cggcgccagc ctgacggccg gatggcgcaa
     6481 caggccgggt agctcggtca gcggcaggtc gagcaggatg gcgaggttga cgaccgactt
     6541 gtacacgtag cgatcgcaga tgacggcccg gccggcgcgc aggtgcggct ggacagcgag
     6601 cgtggcctcg atcaaatgct cgatcagcga gacgtcggtc cagccacgcc gcaggatcca
     6661 gtgcttgcgg aacatcgcgt tcgacttgct caggtactcg ccgaacgctt cggacgcctc
     6721 gctgccggcc gtgcgtgcct cgaagctgcc gtcggagcgt tggaccggcg cgcgcagccg
     6781 gcgcttgacg agtcggacca gcgggcgcga gacgtagggg cggccgcccg tccagacgct
     6841 ggcggtttcc cagccggccg cttccatccg ttctttcagg a
//
LOCUS       DASUGJ010000255        10544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_177313, whole genome shotgun sequence.
ACCESSION   DASUGJ010000255 DASUGJ010000000
VERSION     DASUGJ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10544
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177313"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFV93_05630"
     CDS             <1..732
                     /locus_tag="VFV93_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5164856.1"
                     /translation="HDDTIDMSQLRRGQPTLNSMFDSGTVILIGDYLFAQSAILAAET
                     MNPRVVAVFAASLADICDGQLREVFTAHRLDQTRDDYERRIYGKTASLFAGAAEMGAI
                     IGGAPEEDIQNLRSFGGEVGMAFQIVDDVLDLRSTTDAIGKPANLDLRQGTVTLPTML
                     LLSRLDGNPAAREIERVVNGVDVSDADYAAVASMIEESGAIDESVASARTFIERAIDR
                     LNMIEDAAVADQLASFANLALERTS"
     gene            complement(767..1360)
                     /locus_tag="VFV93_05635"
     CDS             complement(767..1360)
                     /locus_tag="VFV93_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164857.1"
                     /translation="MFDGAHLIEQVPLQDVDLLQRLNNYFATLELRLRQGQGWLIYNA
                     SGNRAGRINRLLLDRLSATEFPFSHYFLPWRDFALTSYLLQGELQQAPGKREEMSERE
                     RTEFAIATRVSRQTLARLATADLLILSGLTPQHPFEVNYLDETIARRHKSRLATIILT
                     PEQPHELALDVVRLGGDGGEAWERMSTTLYETNLVAV"
     gene            complement(1443..2357)
                     /locus_tag="VFV93_05640"
     CDS             complement(1443..2357)
                     /locus_tag="VFV93_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX5164858.1"
                     /translation="MARPMLLDVDTGVDDAVAIALASRLESHELVAITTVAGNVPVEY
                     STANTQTVVAWMGLDVPIYRGMDEPLVRPLFDAREHHGFDGLGGWKPDVRRADIQLET
                     APEAIVRLARQHRGELTAVFVGPLTNLAVALSLEPRIVEWIDRLVIMGGAFFVPGNTT
                     AAAEFNIYADPEAAAKVARSAFRPIWVPLDVTHRTGVTRSEWDRLANSDEPGYVLVRE
                     VMRQALTERGRPRFHLHDPLAVAVAVDPTIVSTRSGRVLVNVGPFERGRTILTDAIGT
                     EPQGSVAVEVDHDRFETLLKRLVPLGTR"
     gene            complement(2383..4086)
                     /locus_tag="VFV93_05645"
     CDS             complement(2383..4086)
                     /locus_tag="VFV93_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5164859.1"
                     /translation="MNVSEAIVAILAESGVEHIFGLPGDTGMDFYDALYRNEGRIQHI
                     LTRDERSASFMADAYARVTGKLGVCEGPSGGGATYIVPGVAEAHGSCLPLLCLTSDTP
                     VREEGRGVLTELNQPALFAPITKWSARLNTAEMSADIVRRAVRLATTDRPGAVALSMP
                     ADVLKAEVDDANVYGTPQFMAAPASRTRPDPESVNKATDLIRAAKRPVIIAGGGVLIS
                     RAWDALTAFAEAGSMPVGTSINGKGSIAETNRLSLGIVGGNGARPYANDIVAEADLVL
                     LIGTRTDSTTTLNWTLPSKRQPPTVIHIDVDAWQIGNNYRTAVGLIGDARATLEDLLS
                     AFGRQPADGDRTLWLDQIAALKAEYFATVAEEAASDAQPIKPQRVIATLKRLVDAETV
                     LVADPGTPTPFIGAQYELAQAGRWTVIPRAHGGLGYALPAVVGAYHGRPNARTVGLMG
                     DGSFGMSVGELETISRLNLPIVLLHFNNGCFGWIKELQHLYHERRYFSVDFNPVDYSA
                     IARGFGLRAWQVTDPADLEGAMRDALESGAPCFIDMVTEAAMTETPPVHAWLEAAAAQ
                     Q"
     gene            complement(4161..5420)
                     /locus_tag="VFV93_05650"
     CDS             complement(4161..5420)
                     /locus_tag="VFV93_05650"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX5164860.1"
                     /translation="MSSSLRRDIEQIYREALEPVRAETAIRANLSWDSESSLLIGGQS
                     VMIGDEGVYAIAIGKASVAMISAASGVLGGRFTAGVAVTKAEKSPQDSRITVIQGSHP
                     VPDERSLAAGDAVLGFADLIPDGALVLGLISGGGSALVESLRQGVGLDRLRELTSGLL
                     RAGASIHELNAVRSQLSRIKAGGLLDALARTRVHNLIVSDVLGDDVRTIASGPTVPPG
                     STDAASVMRKYGMTGSIPDHATSNERPMPPTTIVASLSMALDAAAEHAVVLGYQPYVL
                     TRSIDSEAREAGRLLATILADSVVSRTSFGPRSCLLAGGETTVTVRGDGVGGRNTEAA
                     LAAAIRLAGVETAAIGFLATDGDDGISGAAGGIVDGGTVTHDVRDAARHALDSNDSYT
                     FLNARGAVLAGGETGTNVNDLVIGLIG"
     gene            complement(5417..6343)
                     /locus_tag="VFV93_05655"
     CDS             complement(5417..6343)
                     /locus_tag="VFV93_05655"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5164861.1"
                     /translation="MSAAETFVVRLEVAPDAASERLDRFITDRMPDTSRSFVQRLIES
                     GGVQVNGALPRPSQRVNAGDSIEVRVPKPAEPVEITPAHVMVPIVFEDDDVIVFDKPA
                     GLVVHPAPGHEHGTLVNVLRWIRPEIVMPGTERPGIVHRLDKDTSGLIVVAKNERARL
                     SLLRQWQKRDVIKDYTALVVGTLADDAATVDAPIGRDPHNRKRMAVVREGRPAISHLT
                     VAARYSGYTLLDVRIETGRTHQIRVHCAFIKHPVAGDSLYGGATRDLELDRQFLHARR
                     LRFALPGGQPVDVEASLPSDLSNVLADLESRT"
     gene            complement(6340..6852)
                     /gene="lspA"
                     /locus_tag="VFV93_05660"
     CDS             complement(6340..6852)
                     /gene="lspA"
                     /locus_tag="VFV93_05660"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5164862.1"
                     /translation="MLVGALALIVTVALDQISKWIVLETIGPNGSRGMVEIIPRVLEF
                     DFVRNTGSAFGMFQGNSDILKILALGAVVALFVYYVRSAKHDWIVSLALGLQLGGALG
                     NILDRFRHGYVVDFIEFPRFPTFNVADSAITVGVFLLMYAVLFRDGHQRDAAERQQAI
                     QHQSVAGDDA"
     gene            6960..8147
                     /gene="murG"
                     /locus_tag="VFV93_05665"
     CDS             6960..8147
                     /gene="murG"
                     /locus_tag="VFV93_05665"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX5164863.1"
                     /translation="MTSESTIGTADRPLRVVIAGGGTGGHVSPGVAVIDELRARLVVE
                     PHWIGSRGGFEHETAERMGIPFHPIQVGKLRRYPSINTLIDAGRIPVGVIQAWRHLRA
                     IRPDLTFATGGYVSTPVVIAGRSLGIPSMTHEQTAHVGLATRINARFVDVVALSYERS
                     LAALGKTRAAFVVTGNPVRELTCNGDAQAAFDRFCFAPDMPLVYVTGGALGAHAINVA
                     VSQALPDLLKCVQILHQCGPQTANSDFTTLTDLAAGLPEDLRRRYHVVERVSDEIGDV
                     FAAASLVVGRAGAGTIAELAAAGLPAILIPLPGAEEQRQNALYLADAGAALVIDQRNL
                     TPALLVERITELIASPQRRSEMRAAGLRSVPPDPAARIVDELLKLAPAASPSTDVTGT
                     RSR"
     gene            complement(8173..9546)
                     /gene="radA"
                     /locus_tag="VFV93_05670"
     CDS             complement(8173..9546)
                     /gene="radA"
                     /locus_tag="VFV93_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX5164864.1"
                     /translation="MAKTRTKYVCQQCGLESPAYYGRCPDCGAWGSLVETIEPKAAPA
                     PRRGGRIAGVAKPEPLSSIKSADITRRPVPIQEFSRVLGGGVVPGSLVLVGGDPGIGK
                     STLLLQAAAGLAGETEPVLYVTAEESAQQVKMRADRLSVALDGLYVLPETNIDTVLAS
                     AEELRPSTIVIDSIQTVYTDEITSAAGSVSQVRECTARLMAFAKPRDVAIFIVGHVTK
                     EGAIAGPRVLEHMVDAVLYLEGERFHQYRILRGVKNRFGSTDEVGVFEMVEAGMREVR
                     NPSEAFLQERAGNAAGSTVTVTMEGTRPILVEVQGLTSTSAYGTARRTANGFDLNRLQ
                     MLIAVLTKRVGIGLADQDIYVNVVGGLRLSEPAVDLGVALAVASSFRETRVSSHLVAL
                     GEVGLSGELRSVGQLERRLQEAASLGFKQALVPATLGRGMVRPPDGLLLTRVSSLREA
                     IEEALGR"
     gene            complement(9624..>10544)
                     /locus_tag="VFV93_05675"
     CDS             complement(9624..>10544)
                     /locus_tag="VFV93_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5164865.1"
                     /translation="QDEAISTMAKAVRRARAGLKDPRRPIGSFIFLGPTGVGKTLLAR
                     ALAEFMFGSEDALIKIDMSEFMERHTISRLVGAPPGYVGYEEGGQLTEAVRRKSYSVI
                     LLDEIEKAHPEAFNMLLQILEDGNLADAKGRRVDFRNTIIIMTSNVGAEQITSDKQFG
                     FQTASEGEKKRQQDYEQMKDRVLGQLKQTFRPEFLNRIDGTIVFHALTNEEIRNIVNL
                     ELVRIRTQLVEQEITLEVTEAAMDLLGERGYDRQYGARPLRRIIQNLIEDPMAEALLE
                     GRYKAGQTIVVDVEDDLLRIIPAETLVTVP"
BASE COUNT         1859 a         3567 c         3256 g         1862 t
ORIGIN      
        1 cacgatgaca cgatcgacat gtcccagctg cggcgcggcc aaccgacgct gaactcgatg
       61 ttcgattccg gaacggtcat cctgatcggc gactacctct tcgcacagtc ggcgatcctc
      121 gccgccgaga cgatgaaccc acgcgtcgtg gccgtatttg ccgcgtcgct ggcagacatc
      181 tgcgatgggc agttgcgcga ggtgttcact gctcaccgtc tcgatcagac ccgcgacgac
      241 tacgagcgcc gcatctacgg taagacggcg tcgctcttcg ccggggcggc tgagatgggc
      301 gcgatcatcg gcggcgcccc cgaagaagat atccagaacc tgcgcagctt cggcggcgag
      361 gtcgggatgg ccttccagat cgtcgacgac gtgctcgatt tgcgctccac aaccgacgcg
      421 atcgggaaac ctgccaacct ggatcttcgc caggggacgg tcaccctgcc gacgatgctg
      481 ctgctatccc ggctggacgg caacccggcc gcccgtgaga tcgagcgagt cgtcaacggt
      541 gtcgatgtct cggacgccga ctacgcggcc gtcgcatcga tgatcgagga gtcaggggcc
      601 atcgacgagt ccgtcgcttc ggcgcgcacc tttatcgagc gggctatcga ccggcttaat
      661 atgatcgagg acgcagcggt tgccgatcag ctggcatcgt tcgcgaacct ggcgttggaa
      721 cgtacctcgt agtaccggcc agagtgcgaa cgctcacagc agcggttcag accgcgacga
      781 ggttggtttc gtagagcgtc gtcgacatcc gctcccaagc ctccccgcca tcgccgccga
      841 gccgaacgac atcgagcgcc agctcgtgtg gttgctccgg cgtcaggatg atcgtcgcca
      901 gcctgctctt gtgccggcgc gcgatcgtct cgtcgaggta attgacctcg aacgggtgct
      961 gcggtgtcag gccgctaaga atcagcagat cggccgtcgc cagcctcgcc agcgtctgcc
     1021 gtgagacgcg cgtcgcaatc gcgaactccg ttcgctcgcg ctcgctcatc tcctcgcgct
     1081 tgcctggcgc ctgctggagc tcgccctgga gcagatacga tgtcagcgcg aagtctctcc
     1141 acggcaggaa gtagtggctg aatgggaact cagtcgcgct aagccggtcg agcagcaggc
     1201 gattgatcct accggcgcgg ttgccgctgg cgttgtagat gagccagccc tgtccctgcc
     1261 gaagccgcag ttcaagcgtg gcgaagtagt tgttcagacg ctggagcaga tcgacatcct
     1321 gtaacggtac ctgctcgatg aggtgagctc cgtcaaacac gtcgcacact cccgttcacc
     1381 tgaatcgtcc cgtcgaccca ttgtgtacgt acgtacggtc cgcgtcaagc agccggatgt
     1441 ccttatcgcg tcccgagtgg cacgagtcgc ttcaggagcg tctcaaaccg gtcgtggtca
     1501 acctcgaccg cgaccgaccc ctgaggctcc gtgccgatcg cgtcggttag tatcgtacgg
     1561 ccgcgctcga acggaccgac gttaaccagc accctgccgg atcgagtaga gacgatcgtc
     1621 gggtcgactg cgactgcgac cgccagtgga tcgtgcaggt ggaagcgcgg gcgaccacgc
     1681 tcggtcagcg cctgccgcat cacctctcgc acaaggacgt accccggctc gtcactgttc
     1741 gccagccgat cccattcgga acgggtaaca ccggtccgat gtgtgacatc gagcggtacc
     1801 cagattggac ggaacgcaga tcgcgccacc ttggcggcgg cctccggatc ggcgtagata
     1861 ttgaactccg cagcagccgt cgtgttgccg ggaacgaaga acgcacctcc catgatgacg
     1921 agccggtcga tccactcgac gatccgaggc tccagcgaca acgccaccgc caggttcgtc
     1981 aacgggccga cgaacactgc cgtcagctcg ccccgatgct gtcgagcgag ccggacaatc
     2041 gcctccggtg cagtctccaa ctggatgtcg gctcggcgaa catcgggctt ccagccgccg
     2101 agcccatcga acccgtggtg ctcgcgggcg tcgaacagcg gccggaccaa tggctcgtcc
     2161 ataccacggt agatcggaac gtcgagtccc atccaggcga caaccgtctg ggtgttggcg
     2221 gtcgaatact cgactggaac gtttccggcc acggtcgtga tcgccaccaa ctcgtgcgac
     2281 tcgagccgcg aggccagcgc gatcgcgacc gcgtcgtcga ctccggtatc gacatccagc
     2341 agcatgggac gggccatcaa cgtcctccgt cagttccgat cgctactgct gcgcagcggc
     2401 cgcttcgagc caggcatgga ccggcggtgt ctcggtcatg gcggcctcgg tcaccatgtc
     2461 gatgaagcat ggcgcgccgg actccagcgc gtcacgcatg gcgccttcga ggtcggcggg
     2521 atcggtcacc tgccaggcac gcaggccgaa gccgcgtgcg atcgcggagt agtcgaccgg
     2581 attgaagtca actgagaagt agcgccgctc gtgatacagg tgctgaagct ccttgatcca
     2641 gccgaagcag ccgttattga agtgcagcag cacgatcggc aggtttagcc gcgagatcgt
     2701 ctccagttcg ccaaccgaca tgccgaacga gccatcgccc atcagcccga ctgtgcgggc
     2761 attcggccgg ccgtggtagg cgccgacgac agccggcagc gcatagccta gcccgccgtg
     2821 cgcgcgaggg atgaccgtcc agcgccctgc ctgcgccaac tcgtattgtg cgccgataaa
     2881 tggcgtcggt gtgccggggt ccgccaccag caccgtctcg gcatcgacca gccgcttcag
     2941 ggtggcgatc acccgctgtg gcttgatcgg ttgggcgtcc gatgccgctt cttccgcgac
     3001 cgtcgcgaaa tactcggctt tcagcgccgc gatctgatca agccagaggg tgcggtcgcc
     3061 gtcggcgggt tgacggccaa acgcagacag gagatcttcg agcgtcgccc gtgcatcgcc
     3121 gatcagtccg accgccgtcc ggtagttgtt cccgatctgc cacgcgtcaa cgtcgatgtg
     3181 gatgacggtc ggtggctgcc gtttggaggg cagagtccag ttgagggtcg tcgtcgaatc
     3241 agtccgcgtc ccgatcagca ggaccagatc ggcctcggcg acgatgtcat tcgcatatgg
     3301 ccgcgccccg ttgccgccga cgatgccgag gctgagccgg ttcgtctcgg cgatgctgcc
     3361 cttgccgttg atcgaagtcc cgaccggcat cgagcccgct tcggcgaatg ccgtgagagc
     3421 atcccaggcc cgcgagatca gtacgccgcc gccggcgatg atgactggcc gcttcgccgc
     3481 gcggatcaga tcggttgcct tgttgaccga ctctgggtcg ggacgcgttc gcgacgccgg
     3541 cgccgccatg aactgcggcg tgccatacac gttcgcatcg tccacttcgg ccttgagcac
     3601 gtcggccggc attgagagcg cgacggctcc cggtcgatcg gtcgtcgcca gccggacggc
     3661 ccggcgcacg atgtcggccg acatctcagc cgtgttgagc cgtgccgacc atttggtgat
     3721 cggcgcgaag agcgcaggct ggttcagctc ggtcagaacg ccgcgcccct cctcgcggac
     3781 cggcgtgtcg gaggtcaggc atagcagcgg caggcaggaa ccgtgcgcct cggcgacacc
     3841 cggcacgatg taggtcgcgc cgccaccgct cgggccttca cagacgccga gcttgccggt
     3901 cacgcgggcg taggcgtcgg ccatgaacga cgcagagcgt tcgtcacggg tcaggatgtg
     3961 ctggatgcgt ccctcgttgc ggtagagtgc gtcgtaaaag tccataccag tatcgcccgg
     4021 caggccaaag atatgctcga cgccactctc ggcgaggatc gcgacgatcg cctcgctgac
     4081 attcataccc gctcatccct tccgcagtcc ggcctttgcc ggcgtactat agcagaccca
     4141 ctcacgcggg ttgatcgtcg ctagccgatc agtccgatca cgagatcgtt gacgttcgtc
     4201 ccggtctcac cacctgcgag gacggcgccg cgcgcgttca gaaacgtgta ggaatcgttg
     4261 ctgtccagcg catggcgcgc agcgtcgcga acgtcgtgcg tcactgttcc tccgtcgacg
     4321 atcccaccgg cagcgcccga aatcccatca tcgccatcgg tcgccaggaa cccgatggcc
     4381 gcagtctcga caccggccag ccggatcgcc gccgccaacg ccgcctcggt attgcgcccg
     4441 ccgacaccat ctccacgaac cgtcaccgtc gtctcgcccc ctgccagcag gcacgagcgc
     4501 gggccgaacg acgtccggct caccacacta tctgccagga tggttgccag caatcgcccc
     4561 gcttcgcgcg cttcgctgtc gatcgatcga gtcaggacat atggctggta gccaagcacc
     4621 accgcgtgtt cagccgcagc gtcgagcgcc atcgacaggc ttgcgacgat cgtcgttggt
     4681 ggcatcggcc gctcgttcga agtcgcgtga tccgggatgg aaccggtcat cccatacttc
     4741 cgcatcaccg acgcagcgtc agtcgaacca ggagggacgg tcggcccact cgcgatcgtt
     4801 cgaacgtcgt ctccaagcac atcggagacg atcagattgt gaacacgcgt ccgtgcgagc
     4861 gcgtcaagca gtccacccgc cttgatccgg ctcagctgcg accgaaccgc gttgagttcg
     4921 tgaatggagg caccagctcg tagcagtccg ctcgtcaact cccgcagccg gtcaagaccg
     4981 acgccttgcc gcaagctctc caccagcgcc gatccgccgc cggagatcag gccgagcacc
     5041 aacgcaccgt ccggtatcaa gtccgcgaaa cccagcaccg catcaccagc cgccagcgac
     5101 cgctcatctg gtaccggatg tgaaccctgg atcacggtga tccggctgtc ttgtggcgac
     5161 ttctctgcct ttgtcacggc aacaccggcc gtgaaccgac ctccaagcac gccggaagcg
     5221 gcggagatca tcgctacgga agccttgcca attgcgatgg catagacccc ttcgtcacca
     5281 atcatgacgc tttgtccgcc gatcaacaga gaggattcgg agtcccagga caggttcgcg
     5341 cgaatggcag tttcagcccg tacgggctcc agcgcttccc gatagatctg ttcgatgtct
     5401 cggcgcagtg aactactcat gtgcgcgatt cgaggtctgc cagcacgttc gataggtccg
     5461 agggcaggga cgcctcgaca tcgaccggtt gaccgcccgg gagtgcgaag cgcagccgtc
     5521 gcgcgtgcag aaattggcgg tccaactcca gatcgcgggt cgcgccgcca tagagcgagt
     5581 ccccggcgac cggatgcttg atgaacgcgc agtgcacgcg gatctgatgc gtgcggccgg
     5641 tttcgatccg gacgtccagg agtgtgtagc cagaataccg cgctgcaacc gtcagatggc
     5701 tgatggctgg gcgcccttca cggacgactg ccatccgctt ccggttgtgt ggatcccgtc
     5761 cgatgggtgc atcgaccgtc gccgcatcat ctgcgagcgt gccaaccacc aatgccgtgt
     5821 agtccttgat tacgtcgcgc ttctgccact gccgcagcag cgacagccgc gcccgctcgt
     5881 tcttggcgac gacgatcagg ccagaggtgt ccttgtcgag gcggtggacg ataccgggcc
     5941 gctccgttcc cggcatgacg atctctggcc ggatccagcg caggacattg acgagcgtac
     6001 catgctcgtg gcccggcgcc ggatgaacga ccaacccggc cggcttgtcg aagacgatca
     6061 cgtcgtcgtc ctcgaacacg atcgggacca tgacatgtgc cggcgtgatc tcgaccggtt
     6121 cagccggctt cggcacccgc acctcgattg agtcaccggc gttcacccgc tggctgggac
     6181 gcggcaacgc gccgttgacc tgcactccgc ctgactcgat cagccgctga acaaacgacc
     6241 gcgacgtgtc gggcatccgg tcggtgatga agcggtccaa ccgttcggag gcggcgtctg
     6301 gagcgacttc gagccgcacg acgaacgttt ctgccgcgct cacgcgtcgt cccccgcaac
     6361 gctttggtgc tggattgcct gctgtcgctc cgcggcgtcg cgttggtgac cgtcgcgaaa
     6421 cagcacggcg tacatcaaca ggaacacgcc gaccgtgatg gccgagtcag cgacgttgaa
     6481 ggtcgggaat ctggggaact caatgaaatc aacgacgtag ccgtgcctga accggtcaag
     6541 gatgttcccg agcgcgccgc cgagttgcag gccgagcgcc agcgatacga tccagtcatg
     6601 tttggcagag cgcacatagt aaacgaacaa cgcgacgacg gcacccagtg ccagaatctt
     6661 gaggatgtcc gaattgccct ggaacatgcc aaacgctgag ccggtgttgc ggacaaagtc
     6721 gaactcgagc acgcgcggaa tgatctcgac catcccgcgc gagccgtttg gcccgatcgt
     6781 ttcaagaacg atccatttcg agatctggtc aagcgcgacc gtcacgatga gtgccagcgc
     6841 tccgaccagc aacgaccgtc gcgttgtgac gctttgagtg gacagcctga ttatgtgaca
     6901 ctcctagatg cgagtttgga ggtgcgcccg gcgcgcaccc gagcaggagg cgcctacccg
     6961 tgacttcgga gtctaccatc ggtaccgccg accggccgct gcgcgtcgtc atcgccggcg
     7021 gcggcacagg aggccatgta tcaccgggcg tcgccgtcat cgatgaactg cgcgcgcgcc
     7081 tcgtcgtcga accgcactgg atcggttcac gcggtggatt cgagcacgaa accgccgagc
     7141 ggatgggcat tccatttcat ccgattcagg ttggcaaact tcgtcgttat ccgtcgatca
     7201 acaccctcat cgacgcaggt cggatcccag tcggcgtcat ccaggcctgg cgacacctgc
     7261 gcgcgatccg gccggacctg accttcgcga ccggcggcta cgtcagcacg ccagtcgtca
     7321 tcgccgggag gtcgctcggt atcccctcga tgacacacga acagaccgcg cacgtcgggc
     7381 ttgctacgcg catcaacgca cgcttcgtcg atgtcgtcgc gctgtcgtac gagcgctcgc
     7441 tggccgcgct tggaaaaact cgcgcagcct tcgtcgtcac cggcaatccg gtccgcgagc
     7501 tgacctgcaa cggcgacgcc caggcagcgt tcgaccggtt ctgcttcgcg cccgacatgc
     7561 cgctcgtcta cgtcactggc ggcgcactcg gagcccacgc catcaacgtc gccgtttctc
     7621 aagcattgcc ggatttgctg aagtgcgtcc agatccttca ccaatgcgga ccgcaaaccg
     7681 ccaacagcga cttcacgacg ctgacagacc tcgcggccgg cttgcccgag gacctacgcc
     7741 gacgctatca cgtcgtcgag cgcgtcagcg acgagatcgg agacgtgttc gcggccgcgt
     7801 cgctggtcgt cggccgtgcc ggagccggca caatcgccga actggccgcc gccggcctgc
     7861 ccgccatcct gatcccctta cccggcgctg aagagcaacg ccagaacgcc ctctacctcg
     7921 ctgacgccgg ggccgcgctc gtgatcgacc aacgcaacct gacacccgcg ctgctggtcg
     7981 aacgaatcac cgagctgatc gcctcgccgc aacgacgctc ggagatgcgt gccgccggac
     8041 tccgaagcgt accgcctgat ccggctgcac gcatcgtcga tgaactgctg aaactcgcac
     8101 ccgctgcatc accatctacc gatgtaactg gcacgcggtc gagatagccc tctgggcgtt
     8161 ggaaaacagg ggctagcgcc ctagcgcctc ctcgatcgcc tcgcggagcg acgacacgcg
     8221 cgtcagcaac agcccgtccg gtggacgcac catcccgcgc ccgagcgtcg ccgggacgag
     8281 cgcttgtttg aatccgaggc tggccgcctc ctgtaagcgc cgttcaagtt ggccgaccga
     8341 acggagttcg cccgagagcc caacttcacc gagggcgacg agatgtgaac tgacacgcgt
     8401 ttcgcggaag ctggacgcta ctgccagcgc gacacccaga tcgacggccg gctcggagag
     8461 ccgcagaccg ccgacgacgt tgacgtagat gtcctggtca gccagaccga tccccacccg
     8521 cttggtcaga acagcgatca gcatctgcag ccggttgagg tcgaagccgt tcgcggttcg
     8581 cctggccgtc ccgtatgcac tggtcgatgt cagcccctgg acttcgacca gtatcggccg
     8641 ggtgccctcc attgtcacgg tgacggtcga accggccgcg ttgccggctc gctcttgcag
     8701 gaacgcttcg ctgggattgc gtacctcacg catgccggcc tcgaccatct cgaagacgcc
     8761 gacttcgtcg gtcgagccaa agcggttctt gacgccgcgc agaatgcggt actggtggaa
     8821 gcgttcgcct tcgagataga ggacggcgtc gaccatgtgt tccagcacgc gcggtccggc
     8881 gatcgcgccc tccttggtga cgtggccgac gatgaagatc gccacgtcac gtggtttcgc
     8941 aaacgccatc agccgcgccg tgcattcgcg cacctggctg acgctgccgg ctgccgaggt
     9001 gatctcgtcc gtatagacgg tctggatcga gtcgatcacg atcgttgagg gccgtagctc
     9061 ctccgccgac gccagaaccg tgtcgatgtt cgtctccggc aggacgtaga gcccgtcgag
     9121 cgcgacgctg agccggtcgg ctcgcatctt gacctgctgg gccgactcct ccgccgtcac
     9181 atacagcacc ggctccgtct cgcctgccag cccggcggca gcctggagca gcagcgtcga
     9241 cttgccgatg ccaggatcac ctccgaccag cacgagcgaa ccgggtacga caccgccgcc
     9301 gagaacccgg gagaactcct ggatcggcac cggccggcgg gtaatgtccg cactcttgat
     9361 cgacgacagc ggctcaggct tggcgacgcc ggctatccgc ccgccacgcc ggggcgcagg
     9421 tgctgccttc ggctcgatcg tctcgaccag cgatccccac gccccacagt cggggcagcg
     9481 accgtaatag gccggactct cgaggccaca ttgctggcaa acgtatttgg tccgtgtctt
     9541 ggccacgtcg atgctttctg gatcaaacga atgacgggga cgcctgcgcg tccccgtccg
     9601 ggtcaatcat ctggaagcgc gagttacggt accgtcacca gcgtctcggc cgggatgatc
     9661 cgcagcaggt catcctcaac gtcgacgacg atcgtctggc cggccttgta gcgtccttcg
     9721 agcaacgctt cggccatcgg atcctcgatc aggttctgga tgatgcgccg cagcgggcgg
     9781 gcaccgtact gccggtcgta gccgcgctcg ccaagcaggt ccatcgccgc ctcggtcact
     9841 tcgagcgtga tctcctgctc aaccagctgg gttcgaatcc ggaccagttc cagattgacg
     9901 atgttgcgga tctcttcgtt cgtcagcgca tggaagacga tcgtgccgtc gatccggttc
     9961 aggaactcgg gccggaacgt ctgcttgagc tggccgagca cacgatcctt catctgctcg
    10021 tagtcctgct gccgcttctt ctcaccttcg ctggccgtct ggaaaccaaa ctgcttgtcg
    10081 ctggtgatct gctcggcgcc gacgttcgag gtcatgatga tgatcgtgtt gcggaagtcc
    10141 acgcgccggc ccttggcgtc cgccaggtta ccgtcctcaa ggatctgcaa cagcatgttg
    10201 aacgcttcgg ggtgcgcctt ctcgatctcg tcgagcagga tgacagagta gctcttgcgc
    10261 cgaacggcct cagtcaactg gccgccctct tcgtagccaa cgtagcccgg aggcgcgccg
    10321 accagccgcg agatggtgtg gcgctccatg aactcgctca tgtcgatctt gatcagcgcg
    10381 tcttcggagc cgaacatgaa ctcggccagc gcccgcgcca gcagcgtctt gccgacaccg
    10441 gtcgggccga ggaagatgaa cgagccgatc ggccggcgcg ggtccttcag gccggcgcgt
    10501 gcgcgacgga cggccttcgc catcgtcgag atcgcctcgt cctg
//
LOCUS       DASUGJ010000256         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1983806, whole genome shotgun sequence.
ACCESSION   DASUGJ010000256 DASUGJ010000000
VERSION     DASUGJ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983806"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VFV93_05680"
     CDS             <1..853
                     /locus_tag="VFV93_05680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5164866.1"
                     /translation="TAPSTATATTPPVETPTQPAAPATAPTATLEPTATTSPTAISEP
                     TATPPPTSTPEPTVTPTPTVPAPTATQAASDAFITIQTADTDEKIVALTFDAGADRGY
                     AEEILDTLQAEGIHATFGMTANWAEANPDLILRMVNEGHVLINHTETHRSWTGFSTGA
                     PPLTSAERADELHATEAVVESIAGYDLRPYFRPPYGDYDDTVVADLKANGYSVNVMWT
                     VDSLGWNGLSAPDITARVINGTVPGAIHLFHVGAASQDAAALPDIIAGLRDLGYRFVT
                     IKEMVGR"
     gene            complement(865..1104)
                     /locus_tag="VFV93_05685"
     CDS             complement(865..1104)
                     /locus_tag="VFV93_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164867.1"
                     /translation="MGVSRVWLGFDVGVGVVIAVALAVEGRTAQGAVIALAVVPVLML
                     FSALRTIQMRYIDRKAQTIMDGVPPRERARHGDQV"
     gene            1305..>2638
                     /locus_tag="VFV93_05690"
     CDS             1305..>2638
                     /locus_tag="VFV93_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164868.1"
                     /translation="MPDSRRPIEIDDLYNLIFVADPNISPDGARVAWVATTVDRNKNG
                     YRSSIWIAPSDGSEAPYRFTGGTGKDTSPRWSPDGSQIAFLSDRSGKNQVWVIPVDGG
                     EAWQVTDGPNAASEIGWMPDGASIYYTTKVEPEEGDERKSDTRVITTLRYKADGEGFL
                     DNKRRHIFAASVDGGESRPLTSGDFDCSQPAVSPDGRYLACMSNRSDDNRNNSLSDIW
                     VTDLHNNESRRVTPEDGNYSTPAWSTSGEVAFVGHPIVEPYGPSTLDNLYTWSPDTDE
                     INGLLVGIDREPGNGAIADARYGVPTQPPIWSSDGSQVYTLLSDRGSVDIYTCPASGN
                     PSSLAGGQRDIQSFTVANDGTISFAASTMQLPTEVFVRRPDGSETQLSHHNDDFINGI
                     ELGSIEEVTFESDPGVEIHGWLLRPPAFNADAKYPAILEIHGGPHGMYGVGF"
BASE COUNT          538 a          997 c          705 g          398 t
ORIGIN      
        1 aacggcacca tcgacagcga ccgccacaac cccaccggta gagacgccga cgcaaccagc
       61 tgctccagcc accgcaccga ccgcgacgtt ggagccgaca gcaacgacgt ccccaaccgc
      121 gatcagcgag ccgactgcta cgccgcctcc taccagcacg cccgagccga ccgtcactcc
      181 aacgccaacg gtgcctgcgc cgacagccac ccaggcagcg tcggacgcct tcatcactat
      241 ccagactgcc gacaccgacg agaagatcgt cgccctcacg ttcgacgccg gcgccgaccg
      301 tggatatgct gaagagattc tcgacacgct ccaggcagaa ggcatccacg caaccttcgg
      361 gatgaccgcc aactgggccg aagccaaccc cgatctgatc ctgcggatgg tcaacgaggg
      421 ccacgtcctc atcaaccaca ccgagaccca ccgctcctgg accggcttct cgaccggggc
      481 accaccactg accagcgccg agcgtgccga tgagctgcac gcgaccgaag ctgtcgtcga
      541 atcgatcgcc ggctacgacc tgcgtccgta ctttcgcccg ccctacggtg actacgatga
      601 cacggtcgtc gccgacctca aggccaacgg ctattccgtc aatgtgatgt ggaccgtcga
      661 ttcgctcggc tggaatggcc tcagcgcgcc ggacattacc gcccgcgtca tcaacggcac
      721 tgtcccgggt gcgatccacc tcttccacgt cggcgctgcc tcgcaggacg ccgccgcgct
      781 gccagacatc atcgccggcc tgcgcgacct cggctaccgc ttcgtcacga tcaaagagat
      841 ggtgggccgc tgactcgagt tgccctaaac ctggtctcca tgtcgcgccc gctcgcgcgg
      901 cggcacgcca tccataatcg tctgcgcctt gcggtcgatg tagcgcatct gaatcgttcg
      961 caacgcgctg aacagcatca gcaccggcac gaccgccagc gcgatgacag caccctgcgc
     1021 tgttcgaccc tcgaccgcca gcgcaacagc gatcacgacg ccaacgccga catcgaaccc
     1081 cagccagacg cgcgacacgc ccactcggcg gtccgtgagg tacgccgcca gccacaacac
     1141 cggtattgcg attgccagta gggtgtacac catcgtcgcc cgaccatcca ttccacccag
     1201 ctcgattcct atgcatcata tccgcgtgcg agaaaaccgg cgacagattc gatttcccgg
     1261 ctagaatgcc ccggtaatgc aatcgaccga gggaggatgc ccagatgccc gacagccgca
     1321 gaccgatcga aatcgacgac ctctacaacc tgatcttcgt cgccgatccc aacatctcac
     1381 ccgacggagc ccgcgtcgcc tgggtcgcga caaccgtcga ccgcaacaag aacggctatc
     1441 gttccagcat ctggatcgcg ccgagcgatg gctccgaggc gccctaccgc tttaccggtg
     1501 gcacgggcaa agacaccagc ccgcgctggt cccctgacgg cagccaaatc gccttcctct
     1561 ccgaccgctc cggcaagaac caggtctggg tcatcccggt cgatggcggc gaggcctggc
     1621 aagtcaccga tggtccgaac gccgccagcg agatcggctg gatgcctgac ggcgcctcga
     1681 tctactacac caccaaggtc gaacctgaag aaggcgacga gcgcaaaagc gacacgcgcg
     1741 tcatcaccac cttgcgctac aaggccgacg gcgaagggtt tctcgacaac aagcgtcgcc
     1801 acatcttcgc cgccagcgtc gatggtggcg aatcccgtcc actcaccagc ggcgacttcg
     1861 attgtagcca gccggccgtc tcgccggatg gccgctacct cgcctgcatg tccaaccgca
     1921 gcgacgacaa ccgcaacaac tctctcagcg atatctgggt caccgacctg cacaacaacg
     1981 agtcgcgccg cgtcaccccc gaggacggta actacagcac cccagcctgg tcgacgtccg
     2041 gcgaggttgc cttcgtcggc cacccgatcg tcgagccata cggcccatca acgctcgaca
     2101 acctctacac ctggagtcct gacaccgacg agatcaacgg cctgctggtc ggcatcgacc
     2161 gcgagccggg caacggcgcc atcgccgacg cacgctacgg cgtcccgacc cagccgccaa
     2221 tctggtccag cgacggctca caggtctaca ccctcctctc cgatcgtggc agcgtcgata
     2281 tctacacctg ccctgccagc ggcaacccat cgtcactcgc tggcggccag cgcgacatcc
     2341 agtccttcac ggtcgccaac gacggtacga tctcgtttgc cgccagtacg atgcagctcc
     2401 cgactgaagt cttcgttcgc cgaccggacg gcagcgagac ccaactctcc caccacaatg
     2461 acgacttcat caacggcatc gaactgggca gcatcgaaga agtcaccttc gagagcgatc
     2521 cgggcgtcga gattcacggt tggctcctgc gtccgcccgc cttcaacgcc gacgccaagt
     2581 acccggccat cctcgagatc cacggcgggc cgcatggcat gtacggcgtc ggcttctt
//
LOCUS       DASUGJ010000257         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_38546, whole genome shotgun sequence.
ACCESSION   DASUGJ010000257 DASUGJ010000000
VERSION     DASUGJ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38546"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFV93_05695"
     CDS             <1..764
                     /locus_tag="VFV93_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX5164869.1"
                     /translation="TAGYLVELALSVDNMFVFALIFGMFAVPPAYQHRVLFWGILGAL
                     VMRFLFILVGAAMLDAFHWIIYVFGAFLVFTGIKILRQREHTLDLEHNVALRLFRRFV
                     PMTSEYHGQRFSIVHAGRRLATPLLAVLVLVEATDLVFALDSVPAIFAITDDPFIVYT
                     SNVAAILGLRSLYFLLAGIMDRFHYLKLGLGAVLVFVGVKMAGADIYHLPIAASLGVI
                     VALIGGSVVLSLLRPLEETTPHEHPARLEPTMRPE"
     gene            1056..1748
                     /locus_tag="VFV93_05700"
     CDS             1056..1748
                     /locus_tag="VFV93_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX5164870.1"
                     /translation="MFMFDPLYIILMLPTIGLMLWAQNRVKKTSTKYSQVANQRRISG
                     AEAARLVLDANGLYDVPVERVPGELSDHYDPRTRTLRLSDPVYGSASVAALGIAAHEA
                     GHAIQHAEGYAPLKARSALVPAASIGSNLGWIMVLAGILVGLTGLAWLGVALFAAGTV
                     FALVTLPVEFNASSRALAQLTTLGIVDRTEYDQNKQVLNAAAWTYIAGFAAAAVQLLY
                     YIMLVSGMSRRD"
     gene            1865..2179
                     /locus_tag="VFV93_05705"
     CDS             1865..2179
                     /locus_tag="VFV93_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX5164871.1"
                     /translation="MSTMTDLLCPKCQAPMRVMDRNGVTVERCTECGGIFLDRGELER
                     LMRAEGEYNARTYTDYRRDDDDDDDDDDRYARGYRDDSSHQGRPRKKKKRNFLEDIFD
                     FG"
     gene            2460..>2998
                     /locus_tag="VFV93_05710"
     CDS             2460..>2998
                     /locus_tag="VFV93_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164872.1"
                     /translation="MVGSGSGASPGASVAAAGAVASPAPVSVGAGVASVCGGVVASAG
                     AVGSGDGVAAGGDGVTVGGTTVAVGGTGVAVGSGVGGGGSVAFDSSASSGFAFGVFVG
                     FGVFVGFFSFSGSVGAAVLVGSAVAVAVAVGVSPGAAAGAMMAVSVATSVAASTLAFG
                     PFDPPPPPPFPPNDRAWYPP"
BASE COUNT          428 a         1032 c          965 g          573 t
ORIGIN      
        1 tcacggccgg ctacctggtc gaactggcgc tctcggtcga caacatgttc gtcttcgcgc
       61 tgatcttcgg gatgttcgcc gtcccgccgg cctaccagca ccgcgtcctc ttctggggca
      121 ttctcggtgc gctggtgatg cgcttcctgt tcatcctggt cggcgcggcg atgctggacg
      181 cgttccactg gatcatctac gtcttcggcg cgttcctggt cttcaccgga atcaagatcc
      241 tgcgccagcg cgagcacacg ctggatctgg agcacaacgt cgcgctgcgg ctcttccgtc
      301 gcttcgtgcc gatgacgtcg gagtatcacg gccagcgctt ctcgatcgtc catgccggcc
      361 ggaggctcgc gacgccgttg ctggcggtgc tagtgctcgt cgaagcgacc gatctcgtct
      421 tcgcgctcga ctcggtcccg gcgatcttcg cgatcaccga cgacccgttc atcgtctaca
      481 cctcgaacgt cgccgcgatt ctcggcctgc gctcgctgta cttcctgctg gccgggatca
      541 tggatcgctt ccattatctg aagctcggtc tcggcgcggt actcgtcttc gtcggcgtca
      601 agatggccgg cgcggatatc taccacctgc cgatcgccgc ctcgctcggc gtgatcgttg
      661 cgttgatcgg cggctccgtc gttctctcgc tgctccggcc gctggaggag accacgccgc
      721 acgagcatcc cgcccggctg gagccgacga tgcgccctga gtagcgaccg acacgcgaca
      781 cgtactggct tacagagtga aaggcgcttc cttgccggac cttgaagacc tcggatgttt
      841 cgtgattggt gggttcgctg ttgtcacgat cctgctgctg gcagcctgcg tgatcgggct
      901 ggtaatcgtc ggctcggttc tcatctagtc cccatcccgg aggggcacac cgcaacggcg
      961 cggtgatccg ggaaacgttc cggcgcgaca ggtcgtcgcg ccggctggcg aatcgtcaac
     1021 agtgtcccgg ctcgcggggc gcggaaggaa caacgatgtt catgttcgat ccgctctaca
     1081 tcatcctgat gctgcctacg atcgggctga tgctctgggc acagaatcgc gtcaagaaga
     1141 cgtctacgaa gtactcacag gtggcaaacc agcgccgcat cagcggggcc gaggcagccc
     1201 ggctcgtgct ggacgccaac ggcctctacg acgtgccggt cgagcgcgtg ccgggcgagt
     1261 tgagcgacca ctacgaccca cgaacgcgga cgctgcgcct ctccgacccg gtctacggct
     1321 cggcctcggt cgcggcgctc ggcattgccg cccatgaagc gggccacgcc atccagcacg
     1381 cagagggcta tgcgccgctg aaagcacgca gcgcgctcgt cccggccgcc agtatcggct
     1441 cgaatctggg ctggatcatg gtcctggccg gtatcctggt cggcctcacc ggcctggcct
     1501 ggctcggtgt cgcgctcttc gcggccggca ccgtcttcgc gctggtgacg ctgccggtcg
     1561 agttcaacgc ctcgtcgcgt gcgctggcac agctgacgac gctcggcatc gttgaccgga
     1621 ccgagtacga ccagaacaag caggtcctca acgccgccgc ctggacctac atcgccggct
     1681 tcgctgccgc cgctgtccag ctgctctact acatcatgct cgtctcgggc atgtcgcgcc
     1741 gcgactaacc aaccagagcg ccatcggatc ggtgggacga tggcgctcaa ccaggggacg
     1801 cttcccaccg acaacgacgc ccggatcggc tgaaaccggc cgggcacaca aggagggata
     1861 cacgatgagc acgatgaccg atctgctttg cccgaagtgc caggcgccga tgcgggtgat
     1921 ggaccgcaac ggcgtcaccg tcgagcgctg caccgagtgc ggcggcatct tcctcgatcg
     1981 tggcgagctg gagcggctga tgcgcgccga aggcgagtac aacgcgcgca cctacaccga
     2041 ctaccgccgc gacgacgatg acgacgatga cgacgacgac cgctacgcac gcggctaccg
     2101 ggacgactcg tcccaccagg gccgcccgcg caagaagaag aagcgcaact tcctcgaaga
     2161 catcttcgac ttcggctaac ccgaccgcca cacggcacaa cgttcaggcg gggcaatctg
     2221 ccccgcctgt ctgtttctcc ctgccatccc aggttgtaga ccacaccgat gttgacgcgt
     2281 tggtagctcc ccacgcgacg actcctcacc ctgtcatccc gacgcaggag ggatctcgtg
     2341 aatcgttcga tgcctgacgc gtctgcgagg ccgagatccc tcctgcgtcg ggatgacagc
     2401 gggattacta tccctccggc gtcgcgctcg gttgtggttc gttgctgggc tgggagttgg
     2461 tggtcggttc cggttcaggc gcgtcgcccg gtgcctccgt cgctgcagcc ggcgctgtcg
     2521 cctccccggc acctgtctcg gtcggcgcag gtgtcgcgtc ggtttgcggt ggcgtcgttg
     2581 cctcggcagg cgcggtcggc tcgggcgacg gtgtcgcggc aggcggcgac ggcgtgacag
     2641 tcggcggcac gacggtcgca gtcggcggga ccggcgtggc ggtcggctcc ggcgtcggtg
     2701 gcggaggctc ggttgccttc gattcctcag cgtcgtctgg cttcgccttc ggcgtcttcg
     2761 tcggctttgg cgtcttcgtc ggcttcttct ctttctccgg ctcggtcggt gcggcggtgt
     2821 tggtgggctc ggccgtggca gtcgcggtcg cagtcggcgt ctcacccggc gcggcggccg
     2881 gggcaatgat ggccgtctca gtcgccacct cggtcgccgc ctcgacgctg gcgttcggcc
     2941 cgttcgatcc gcctccgccg ccaccattcc cgccaaacga cagagcctgg tacccgcc
//
LOCUS       DASUGJ010000258         9513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1150334, whole genome shotgun sequence.
ACCESSION   DASUGJ010000258 DASUGJ010000000
VERSION     DASUGJ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150334"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1649)
                     /locus_tag="VFV93_05715"
     CDS             complement(3..1649)
                     /locus_tag="VFV93_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164873.1"
                     /translation="MSMSSSLAGRVNENNHKWFTLIAACFALFMAILDNLVVNVALPT
                     ISRDLEASTTQLQWIVSAYILVFASLQITAGGLGDRLGRKRWFMLGIAFFTGSSIFAA
                     LSQNVEMLILARALQGLGAAFILPLSLSLVSNAFPPEERGKALGIWSAVSVSGLALGP
                     VVGGLLVQYVSWHWIFLINVPIGILAIIVAKAVVTESKDTTGEVATDIPGTFLITGAI
                     ASLTWALIEAGNRGWNDSLILGAFGLAAILASAFLFVESRTEKPMVPLRFFKSSTFTG
                     ANMDAFAISFLIAGLAFYGTLYLQNVLGFSPVRAGMALIPMVIVMMFGAPLSGSLVNR
                     VGARGLISFGMIVAGAGTLLYTRADVNGSYWDLLPSYLVMGLGMSFIFAPMTTAVLNS
                     VESERSGVASAVNGAIREIGNAFGIAFLGTIMNRTYQDAFNADTSEHALRSDPNSSAL
                     SPVFDLVSSGMNYGGRVIQDAHIFPGVQQFPDVVATIRLASGNAFMEGMDRAIFVSAF
                     TIMAVAVVSFFLIKNEVAAGVAHTSEPATELELEYEPAAD"
     gene            2074..3216
                     /locus_tag="VFV93_05720"
     CDS             2074..3216
                     /locus_tag="VFV93_05720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164874.1"
                     /translation="MAAFAHFSHDLYPSFIGPLVPAIQDKLNISLAMASLMIPAQQMP
                     SILQPFIGLLADRTSRRWFVVISPATAAISLSSLGLAPNIAVVLLLLLVSGLSSATFH
                     APTVALVGEYGGNKMGRAMAIFMAGGEVARTIGPILITGAIALLTLEGSFVVMIVGIV
                     ASVVLYLTLDTTVTDARRQAGAKVDFKPLLRARRGPISGLLGYSILTGVATTPFHYFL
                     VKLLVEKGHGEWYGGFALSTLFAAGVVGGLVGGSLSDRVGRRAMLVGFTAATPPLLYL
                     YLWAENGTWWVLGLLAVAGAITMAPRSVTLAVGAELLPEARGPMAGLLLALGFVSQSL
                     AALAFGAFADQQGIIDAYWFIPAIWFLSLPFILLLPRRGAPLAMPA"
     gene            3244..3855
                     /locus_tag="VFV93_05725"
     CDS             3244..3855
                     /locus_tag="VFV93_05725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5164875.1"
                     /translation="MEPRELVDLKLAMEFGVAPEGETLPPARLNADGLPRLLVSRFAG
                     GEAIYFRFDVDRRVRDELRALGTERLMTDESQVRSILDRTALVEKLYRVRWYTIERLP
                     QPVEYPDATFQDGRYVVLVDGKVAAWAQTDAESPVAAEVSIETLEAYQRRGFARQVTA
                     AWAAGVLSAGKVAYYSHLLHNTASQAVAASLGLTHLSDEVEYI"
     gene            3935..4168
                     /locus_tag="VFV93_05730"
     CDS             3935..4168
                     /locus_tag="VFV93_05730"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX5164876.1"
                     /translation="MIDTSQIKQHMPVVCSEDGQFATVDHMDRNDSIKLTNDDRGQHQ
                     WIPADWVTRVDDKVHVDRPGKQAMREWYTDVPQ"
     gene            complement(4263..5675)
                     /locus_tag="VFV93_05735"
     CDS             complement(4263..5675)
                     /locus_tag="VFV93_05735"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5164877.1"
                     /translation="MDEQDICYLSAMELRERFRRRELSPLEVTGQILERIGRLNPSLN
                     AFLTVTVERALDDARAAEHAYANGDETGPLCGIPISIKDLTPTAGIRTTMGSLLYEDW
                     VPDEDGPFVERVNAAGAVMLGKTNTPEFGWKGDSGNRLIGPTQNPWKQGATAGGSSGG
                     AAAAVASGLGPLAQGSDGAGSIRIPSGFCGIFGIKPTLGLVAYYPPSSVEVLSHLGPM
                     TRTVRDAALLLNVVGRQDDRDRNSYPANTDFVAACDGGIAGLRVAWSPDLGYAQVDPE
                     VREITARAARRLSDLGAHVEEVDPGIPDPWATLDTIWLAAQAGRHKDDFDRVRELIDP
                     GRIELVERGRQLSAVDLQLALTQRTTIYQQWRRFFEDGNWDLLVTPQLPVTAFPAGDD
                     HPGTINGQPVNYLGWTAFTYPFNLTGQPAATVPCGFAPNGLPVCLQFVGRWRDDATVL
                     RAAAAYEEMAPWAQHRPPVD"
     gene            complement(5790..6656)
                     /locus_tag="VFV93_05740"
     CDS             complement(5790..6656)
                     /locus_tag="VFV93_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5164878.1"
                     /translation="MAESELVILGSAQDGGLPHAGCLCPNCRAARADHTRRRLPASAG
                     IVSGDQLALIDATSAFEEQYHMLWERSARSKDFSGERYGAPEIVVLTHAHTGHYTGLW
                     QLDRSVLAARGTRVLSPPKMYAFLAAQEPWAGMQREGFIVLEPLPLDTAFELLPGITL
                     TAVEVPHRAEWETDTVALLIAGPNAITLYLPDIDRWDEWEHDIASIVSTVDAAFLDGC
                     FWNAPTSRNVPHPPVVETLERLQPLANAGKRIAFTHLNHSNPLCDPSNIAHRQVLARG
                     YEVAREGDGARL"
     gene            complement(6678..7478)
                     /locus_tag="VFV93_05745"
     CDS             complement(6678..7478)
                     /locus_tag="VFV93_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX5164879.1"
                     /translation="MTSLSTVKLAPEQIQQLEELAGVIRTRPPLVAFTGAGISTESGI
                     PDYRGPNGLWTTGSAKPVTYQEFISSEEARLAWWHALPGRVAQNTRPVYNAGHLALAA
                     LERAGALEAIVTQNIDGLHVESGSEPERVIELHGNTRTIRCTNCGRIYPIENFIERAA
                     SLTAPPPCPNCDGILKSGTVAFGQPMPQAELLRALTIAQQAGVMLVVGSTLLVNPAAR
                     VPAVAKASGAYLAIVNIGETALDDEADLRIEAPAGPALTVLAEMLLTT"
     gene            complement(7527..8636)
                     /locus_tag="VFV93_05750"
     CDS             complement(7527..8636)
                     /locus_tag="VFV93_05750"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5164880.1"
                     /translation="MTTIKHEPIIDCDLHQTTRSPKDLYPYLPRAYQERIDLFGTGLS
                     QRVGYPNGGDRGSRADAWPEGGGPAGSDLDLLRSQHLDRYDVEYGILLGQEYRPIPTL
                     PDTDYANALARAYNDWMIEHWIERDERLRGCAIIATQEPKLAAQEIHRIGTHEGIVGI
                     LVPNGARFPYGQRFYDPIFEACVELDLPFVLHTGSEGAGMNGQPTPVGYPSYYIELRQ
                     GRALAYQSHLASMVFEGLFERYPTLTVVFVEGGFTWLPTYLWRLDADWKALGRETPWV
                     KRPPSEYVYEHCRYTTQPMESPDNKQDLLKVLEWGRAEQTLLFASDYPHFDFDSPETS
                     LPPMPETMRRRVFSENAREVFKLPVRQRSVELTAD"
     gene            complement(8651..9064)
                     /locus_tag="VFV93_05755"
     CDS             complement(8651..9064)
                     /locus_tag="VFV93_05755"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX5164881.1"
                     /translation="MSRHVIARLREFPPGSRRIVDVAGRSIGVFNIRGELFALRSSCP
                     HQGAPLCLGIILGRTDASRPFEVTYRDDQDVIKCPWHGWEFDIRTGRSVFNPHRVRVR
                     SYDVTVERDVSPDDADPSVETFDVTVEDGFVVLHA"
     gene            complement(9126..>9513)
                     /locus_tag="VFV93_05760"
     CDS             complement(9126..>9513)
                     /locus_tag="VFV93_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5164882.1"
                     /translation="LALIVLPAPVVVSLAYATRRISGSMAWPLESAHVGGLLPASARA
                     HAFGMRSASWNIGYAFAAFISGQIIARTGSYTPAYVSLGVFCVLSVVVYIVAYGGRLP
                     ADEPAEPKPATVSEALTRSETPAATG"
BASE COUNT         1629 a         3055 c         3170 g         1659 t
ORIGIN      
        1 ggtcagtcag cggccggctc gtactccagt tcgagttcgg tggccggttc cgaggtgtgt
       61 gccacgccgg cggcgacctc gttcttgatc aggaagaacg agacgacggc gacggccatg
      121 atcgtgaagg ccgagacgaa gatcgcgcgg tccatgcctt ccatgaaggc gttgccgctg
      181 gcaagacgga tcgtcgcgac cacgtcaggg aactgctgga cgcccgggaa gatgtgcgcg
      241 tcctggatta cccggccccc gtagttcatg cctgagctga ccaggtcgaa gaccggcgac
      301 agcgccgacg agttcgggtc ggaccgcagt gcatgctccg acgtgtcagc gttgaaagcg
      361 tcctgatagg ttcggttcat gatcgttccg aggaaggcaa tcccgaaggc gttgccgatc
      421 tcgcggatgg caccgttgac ggccgaggcg acgccgctgc gctctgattc gacactgttc
      481 agaaccgccg ttgtcatcgg cgcgaagatg aacgacatcc cgaggcccat caccaggtag
      541 gaggggagca ggtcccagta gctgccgttg acgtcggccc gtgtgtagag cagcgtcccg
      601 gctccggcga cgatcatccc gaaggagatc aggccgcggg cgccgacccg gttcaccaac
      661 gagccggaga gcggcgcgcc aaacatcatc acgatcacca tcggaatcag tgccattccg
      721 gcccggaccg gcgagaagcc gagcacgttc tggagataca gcgtgccgta gaaggccaga
      781 ccggcgatca ggaacgagat cgcgaaggcg tccatgttcg cgccggtgaa ggtcgacgac
      841 ttgaagaagc gcagcgggac catcggcttc tcggttcgtg actcgacgaa gaggaacgcc
      901 gaggcgagga tggccgccag gccgaacgcc ccgaggatca gcgaatcgtt ccagccacgg
      961 ttgccggcct cgatcagcgc ccacgtcaac gaggcgatcg caccagtaat cagaaacgtc
     1021 ccgggaatgt cggtcgcgac ctcaccggtc gtgtccttcg attccgtcac gactgccttg
     1081 gcgacgatga tcgccagaat gccgatcggg acgttgatca ggaagatcca gtgccacgag
     1141 acgtattgga cgagcaaccc gccgacgacc gggccgagcg ccaggccgga gacggagacg
     1201 gcagaccaga tgccgagcgc cttgccacgc tcttccggcg ggaatgcgtt cgacaccagc
     1261 gagagcgaca gcggcaggat gaaggccgcg ccgaggccct ggagcgcgcg cgccagaatc
     1321 agcatctcga cgttctgcga cagcgccgcg aagatcgacg agccggtaaa gaaggcgatg
     1381 cccagcatga accaccgctt gcgtccgagc cggtcgccga ggccgccggc tgtgatctgg
     1441 agcgaggcga agaccaggat gtaggccgaa acgatccact gtagttgcgt tgtcgacgct
     1501 tcgaggtcgc gcgagatggt tggcagcgcg acgttgacga ccagattgtc aagaatggcc
     1561 atgaacagcg cgaagcacgc tgcgatcaac gtgaaccact tgtggttgtt ctcgttgacg
     1621 cgaccggcca gtgacgagct catactcatc ggaattcccc aatcgcttcg atgcgcggaa
     1681 gcggtctcgc ttccgttggc gacctccccg tcgccgtaga ccgcacggtt catcgtgctt
     1741 gtgagcgcaa caacaaacgg ttcaggagaa caaggcacac cggtctgacg gacggcacat
     1801 cgctgcgccc gacgggctcc tggcgtctgc ttacgtacag gctgattcta ggttcgtgtc
     1861 tggtcggtgt gactgtccat tgggataggg aaggacaggt atttcggagt ccgtgacaac
     1921 ctgtacctat gacggataag atgccccctg ttatcgggaa ggggatcgaa gacgaagaat
     1981 ccagcgatcg agacaactgc cggcgagccg gatctgctcg tagtccctgc gccggagccg
     2041 gatgaggata cctacgacca gcgtggcgtc gcggtggcgg cgttcgccca tttctcgcac
     2101 gacctgtatc cgtcgttcat cgggccgctc gtgccggcca ttcaggacaa gctcaacatc
     2161 tcgctggcga tggcgagcct gatgatcccc gcccagcaga tgccgagcat ccttcagccg
     2221 ttcatcggcc tgctggccga ccgcaccagc cggcgctggt tcgtcgtcat ctcgccggcg
     2281 acggccgcca tctcactctc gtcgcttggg ctggcgccga atatcgccgt tgttctgttg
     2341 ctgctgctcg tttccgggct ctcgtcggcg accttccatg cgccgaccgt cgcgctggtc
     2401 ggcgagtacg gcggcaacaa gatgggccgc gcgatggcga tcttcatggc cggcggcgag
     2461 gttgcgagga cgatcgggcc aatcctgatc accggcgcga tcgcccttct gacgctcgaa
     2521 ggctctttcg tcgtgatgat cgtcggcatc gtcgcttcgg tcgtgctcta cctcacgttg
     2581 gacacaaccg tgaccgacgc gcggcggcag gccggagcca aggtcgattt caagccgctc
     2641 ctgcgggcaa ggcgcggtcc aatcagtggt ctgctgggct actcgatcct cactggcgtc
     2701 gcgacgacgc cgttccacta tttcctcgtc aaactgctgg tcgagaaggg ccacggcgag
     2761 tggtacggcg gcttcgcact ctcgacattg ttcgctgccg gcgtcgttgg cggcctggtc
     2821 ggcggcagcc tgtcggaccg cgtcggacga cgagcgatgc tggtcggttt caccgccgca
     2881 acgccgcccc tgctctacct ctatctctgg gccgaaaacg gtacatggtg ggtattggga
     2941 ctgctggcag tcgccggcgc gatcacgatg gcgccccgct cggtgacgct ggcggtcggc
     3001 gcggagttgt tgccggaagc gcgcggcccg atggccggcc tgctgctggc gctcggcttc
     3061 gtctcgcaga gcctcgccgc gctggccttc ggcgcgttcg ccgaccagca aggcatcatc
     3121 gacgcctact ggttcatccc ggccatctgg ttccttagcc tcccgttcat cctgctgctc
     3181 ccccgccgcg gcgcaccgct ggcgatgcct gcctgacacc gcgcgaaggt gagaggtcgg
     3241 aacatggaac cgcgcgaact cgtcgacctg aagctcgcga tggagtttgg cgtcgcacca
     3301 gaaggtgaaa cgcttccacc agctcgcctc aacgcggatg ggctgccacg attgctcgtc
     3361 agccgatttg cgggtggcga ggcgatctac ttccgctttg atgtcgatcg ccgcgtgcgg
     3421 gacgagctac gtgcgctcgg cacggagcgt ttgatgaccg acgagagcca ggtacggtcg
     3481 atcctcgacc gtacagcgct ggtggagaag ctctatcgcg tccgctggta caccatcgag
     3541 cggctccccc aaccggtcga atatcccgat gcaacctttc aagatggacg ttatgtcgtg
     3601 ctggttgacg gcaaggttgc cgcatgggcg caaactgatg ccgagagtcc ggtggccgcc
     3661 gaggtgtcga tcgagacgct ggaagcgtat cagcgccgcg gcttcgcacg gcaggtcacg
     3721 gctgcctggg ctgccggggt cctctcggcc ggcaaggtcg cctactacag ccatctgctg
     3781 cacaacactg cgtcgcaggc ggtcgccgcc agcctggggt tgacccatct gtctgacgaa
     3841 gtcgagtaca tctgacggat tgcggcggct ggtggcatcc gtgctgcgga actccactgg
     3901 gtgattcgac ccagactgtg aggagcacgc ctcgatgata gacaccagcc agatcaagca
     3961 acatatgccg gtcgtctgct cggaggacgg gcagttcgcg acggtcgatc atatggacag
     4021 gaacgactcg atcaagctga ccaacgacga cagaggccag caccaatgga tcccggccga
     4081 ctgggtgacg cgggtcgatg acaaggtcca cgtcgaccgc ccaggcaagc aggccatgcg
     4141 cgagtggtac accgacgttc cgcagtagcc actcccgcga tcaagacgat tgttgccggc
     4201 cccgcgccat gcgcggggcc ggtttccccg tcgtcctaag ccgcagcgaa ggacgacaac
     4261 cttcagtcca caggcggccg gtgttgtgcc cagggcgcca tctcctcgta ggcggctgcc
     4321 gctcggagga ccgttgcgtc gtcgcgccag cggccgacga actggaggca gaccggcaga
     4381 ccgttggggg cgaaaccgca gggaacggtc gcggccggct ggccggtcag gttgaacggg
     4441 taggtgaagg ctgtccagcc gaggtagttc accggctggc cgttgatcgt tcccgggtgg
     4501 tcgtctccgg ccgggaatgc cgtcaccggc agttgtggcg taacaagtag atcccagttg
     4561 ccgtcctcga agaagcgccg ccactgctga tagatggtcg tccgctgggt cagggcgagc
     4621 tgcagatcga ccgccgacaa ctgccggcca cgctcgacca gctcgatccg gcccgggtcg
     4681 attagctctc gcaccctgtc gaaatcgtcc ttatgtcggc cggcctgggc tgccagccag
     4741 atcgtgtcca gcgttgccca aggatcgggg atacctggat cgacctcctc gacgtgcgcg
     4801 cctaggtcgc tcaggcgccg ggcggctctg gccgtgatct cgcgcacttc cggatcgacc
     4861 tgcgcgtagc cgaggtccgg cgaccacgcc accctcaacc ccgcgatgcc cccgtcgcag
     4921 gcggcgacga agtcggtgtt ggccggatac gagttgcggt cgcggtcgtc ctggcggccg
     4981 acgacgttca gcagcagcgc tgcgtcacga acggtgcgcg tcatcgggcc gaggtgcgag
     5041 agaacttcga ccgagctggg cgggtaatag gcgaccagcc cgagcgtcgg cttgataccg
     5101 aagataccgc agaacccgga gggtatccgg atcgagccag ccccgtcgct gccctgcgcc
     5161 agcgggccga gaccggacgc gacggctgcc gccgcgccgc cgcttgaccc gccggccgtc
     5221 gcgccctgct tccacgggtt ctgggtcggc ccgatcagcc ggttgccgga atcacccttc
     5281 cagccgaact ctggtgtgtt cgtcttgccc agcatgactg ccccggccgc gttgacgcgc
     5341 tcgacgaacg gcccgtcctc gtccggtacc caatcctcgt agagcagcga ccccatcgtc
     5401 gtccggatgc ccgccgtcgg tgtcagatcc ttgatcgaga tcgggatccc gcacagcggt
     5461 cccgtctcat cgccattagc atatgcatgc tcggccgccc gcgcgtcatc cagcgcccgt
     5521 tcgacggtca cggtcagaaa cgcattcagc gacggattga gccgcccgat tcgctccagg
     5581 atctgaccgg tcacctccag cggcgacagt tcccgacgcc tgaaccgctc gcgcaactcc
     5641 atcgcggaca ggtaacagat atcctgctcg tccatgatcg acctccgacc gcgttcatcc
     5701 tcaccgctcg tgtcccgcca cgatagcacg gagcgttcaa cctgcgaacc agttggctcg
     5761 catcacgcac gatcagcgca agggccggtc tacaaccgcg ccccatcccc ctcccgcgcg
     5821 acttcgtagc ctcgtgcgag cacctggcgg tgtgcgatgt tggacggatc gcagagcggg
     5881 ttggagtggt tcaggtgggt gaaggcgatg cgcttgccgg cgttggccag tggctggagg
     5941 cgttccagcg tctcgacgac aggcgggtgg gggacgttgc ggctggtggg cgcgttccag
     6001 aagcagccgt cgaggaaggc tgcgtcgacg gtcgagacga tgctggcgat gtcgtgttcc
     6061 cactcgtccc agcgatcgat gtccggtaga tagagtgtga tcgcgttcgg gccggcgatc
     6121 agcagtgcga ccgtgtcggt ctcccactcg gcgcggtgcg gaacctcaac cgccgtcagc
     6181 gtgatgccgg gcagcagttc gaaggctgtg tcgagcggta gcggttcgag gacgatgaag
     6241 ccctcacgct gcatccctgc ccacggctcc tgtgccgcga ggaacgcgta catcttcggg
     6301 ggcgacagca cgcgggtgcc acgcgcggcg agaacggagc ggtcgagttg ccagagaccg
     6361 gtgtaatggc cggtgtgggc gtgggtcagg acgacgatct ccggcgcgcc gtagcgttcg
     6421 ccggagaagt ccttgctacg tgccgagcgc tcccagagca tatggtactg ctcctcgaac
     6481 gcgctggtcg cgtcgatcag ggccagttgg tcgcccgaga cgatcccggc gctggctggg
     6541 agtcgtcgcc gggtgtggtc ggctcgtgcc gcgcgacagt tcgggcagag gcagcccgcg
     6601 tgcggcaggc cgccgtcctg ggcggaaccg agaatcacca gctccgattc agccacgcgg
     6661 ctggcctttc tgttgtttca tgtcgtgagg agcatctcgg ccagaacggt caacgccggg
     6721 ccggctggcg cttcgatccg cagatcggcc tcgtcgtcga gggccgtctc accgatgttg
     6781 acaatcgcca ggtaggcgcc ggacgctttc gcgacagctg gaacccgcgc ggccggattg
     6841 acgagcagcg tcgaaccaac gacgagcatg acgccggcct gctgggcgat cgtgagcgcc
     6901 cgcagtagct ccgcttgcgg cattggttgg ccgaaggcga ccgtgcccga cttcaggatg
     6961 ccgtcgcagt tggggcaggg tggcggcgcc gtcaggctcg cggcacgttc gatgaagttc
     7021 tcgatcggat agatccggcc gcagttcgtg cagcggatcg tgcgcgtatt gccgtgcaac
     7081 tcgatcaccc gctcgggttc cgagccggat tcgacatgca gcccgtcgat gttctgggtg
     7141 acgattgctt cgagcgcgcc ggcgcgctcc agcgccgcca gcgccaggtg accggcatta
     7201 tagaccgggc gagtgttctg agcgacacgc ccaggcagcg cgtgccacca ggccagccgc
     7261 gcctcctcgc tgctgatgaa ctcctgatag gtgacgggct tggcgctgcc ggtcgtccag
     7321 agtccattgg gaccgcgata gtcggggatg cccgactcgg tcgagatacc ggcaccggtg
     7381 aacgccacca gcggcgggcg cgtccggatg actccggcca gttcttccag ttgctggatc
     7441 tgttccggtg cgagcttgac agtagacagg gacgtcattg gatgatgcag cttcccgtta
     7501 ccgttcggtg atgatgcgac ggaatgtcag tctgccgtca attctaccga tcgttgtcga
     7561 accggtagct tgaacacctc gcgagcgttc tcgctgaaca ctcggcgccg catcgtctcc
     7621 ggcatcggcg gcagcgaggt ttccggcgaa tcgaagtcga agtgcgggta gtcggacgcg
     7681 aacagcagcg tctgctcggc gcggccccat tcgaggacct tcagcaggtc ctgcttgttg
     7741 tctggcgact ccatcggctg ggtcgtatag cggcagtgct cgtagacgta ctcgctcggc
     7801 ggacgcttca cccacggcgt ctcgcgaccg agcgctttcc agtcggcgtc gagccgccag
     7861 aggtacgtcg gcagccaggt gaaaccaccc tcgacgaaga cgaccgtcag tgtcgggtaa
     7921 cgctcgaaga gtccctcgaa gaccatgctg gcgaggtggc tctggtacgc cagtgcgcgg
     7981 ccctgacgca attcgatgta atacgacggg tatccgaccg gcgttggctg gccgttcata
     8041 ccagcgccct cgctgccggt atgcagcacg aacggcaggt caagctcaac gcaggcttcg
     8101 aagattgggt cgtagaagcg ttgaccgtac ggaaagcgcg ctccgttcgg aacgaggatg
     8161 ccgacgattc cctcgtgcgt accgatccgg tgaatctcct gggccgccag cttcggttcc
     8221 tgggttgcga tgatggcgca acccctgagg cgctcgtcgc gctcgatcca gtgctcgatc
     8281 atccagtcgt tgtaggcgcg cgccagtgcg ttcgcgtagt cggtgtccgg caacgtcggg
     8341 atcggccggt actcctgtcc gagcaggatg ccgtattcga cgtcgtaacg gtcgaggtgc
     8401 tggcttcgca gcaggtcgag gtccgacccg gccggcccgc ccccttccgg ccaggcgtcg
     8461 gcgcggctgc cacggtcacc gccgttcggg tagccgacgc gctgcgacag gccggtcccg
     8521 aacaggtcga ttcgctcctg ataggcacgc ggcagatacg ggtagaggtc cttgggactg
     8581 cgcgtcgtct gatggaggtc gcagtcgatg atcggctcgt gcttgatggt cgtcatatgg
     8641 ctctcccatc tcaggcgtgc aggacgacga agccgtcctc gaccgtcaca tcgaacgttt
     8701 caacagatgg gtctgcgtcg tcgggcgaga catcgcgctc gacagtcacg tcgtagctgc
     8761 ggacgcggac gcggtgggga ttgaagaccg acctgcccgt gcggatgtca aactcccagc
     8821 cgtgccaggg gcacttgatg acatcctgat cgtcgcgata cgtcacctca aatggccgtg
     8881 aggcgtccgt gcggccgagg atgatgccga ggcagagcgg cgcgccctgg tgcgggcaac
     8941 tgctgcgcag cgcgaacagc tcgccacgaa tgttaaagac gccgatcgag cggcccgcca
     9001 cgtcgacgat ccggcgtgaa cccggtggaa attcgcgcag ccgcgcgatg acatgcctgc
     9061 tcatccggcc ccacctccct ggccggcctg agtctaaccc tgtcctgccg cgtgggcaac
     9121 gcaccttagc cggtcgcggc cggcgtttcc gaacgcgtca gcgcctccga gacagtggcc
     9181 ggcttcggct cggccggctc gtcggccggc agcctgccgc cgtaggcgac gatgtagacg
     9241 acgaccgaga ggacgcagaa tacgcccaac gagacgtagg ccggcgtgta gctgccggtc
     9301 cgggcgatga tctggccgga gatgaaggcc gcgaacgcgt aaccgatgtt ccacgatgcc
     9361 gagcgcatcc cgaatgcgtg cgcccgcgcc gaggccggca gcagaccgcc gacgtgcgcg
     9421 gactccagcg gccaggccat cgatccggag atcctgcgtg tcgcgtaggc gagtgagacg
     9481 acgaccggcg ccggcaagac gatcagcgcc agg
//
LOCUS       DASUGJ010000259         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1706519, whole genome shotgun sequence.
ACCESSION   DASUGJ010000259 DASUGJ010000000
VERSION     DASUGJ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706519"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /gene="rpsB"
                     /locus_tag="VFV93_05765"
     CDS             complement(<1..809)
                     /gene="rpsB"
                     /locus_tag="VFV93_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX5164883.1"
                     /translation="MVSTMSREDTQVTMKALLEAGVHFGHQTKRWNPKMRPYIFGERN
                     GIHVIDLHQTARLLGEAQDFVADIAARNGKIVFVGTKKQAQATVEQEASRCGMFFVNR
                     RWLGGTLTNFVTIRSRLQYLRNLERQSMSGELNLLPKQEATAKEQELEKLQRSLGGLK
                     ELTQRPAAIVVVDPRREELAVKEASRLGIPIIALVDTNCDPDPIDYVIPANDDAIRSV
                     RLIVSRLADAIIEGRTRHEVAISEQEETPTAADYGYPDEESDGDEDSNGQD"
     gene            complement(964..1770)
                     /locus_tag="VFV93_05770"
     CDS             complement(964..1770)
                     /locus_tag="VFV93_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5164884.1"
                     /translation="MGNYWEASQTSGPVPYIPLKPVGGNSESATLNASGDSGFRAGLT
                     TPGGQLIFPVSGSSGYPGDPNDEDIPPPDSRSPEQRGRERQLDYYTEERYWTDYVRIG
                     LPILGVILILLVALLWIIPFLNGDDDDDGTLGAGGTATTTIPVIQPSVTATNQASGSE
                     TPRITLTTQPGATTPPNTGGAPTPTTPSEAPSGEIYEGAVVAVANTGGTGVNMRATAS
                     VDGEIVTVVLDGTEFTTTGPAEEADGYVWWPVTGEAGTGYIVADFLTLVQ"
     gene            complement(2077..2625)
                     /locus_tag="VFV93_05775"
     CDS             complement(2077..2625)
                     /locus_tag="VFV93_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5164885.1"
                     /translation="MSFTSTAGSATMDRSATGMTIIDANTVIDGTLSTSHDLRIDGHV
                     TGTVMCDGVLHVTEGAEVDATVEAAGIVVAGSLSGAITCHGRLEIRSTGMVRGRVETG
                     RLIVVEGAVYEGQLRMDAEQVEPTPLTPLPTQVAEAEPEAAAPGQATGSYSFLRSFTP
                     GSTPAREDDDDLPASPGDEDPE"
     gene            complement(2669..>2931)
                     /locus_tag="VFV93_05780"
     CDS             complement(2669..>2931)
                     /locus_tag="VFV93_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5164886.1"
                     /translation="IFGEVEGELAANNEIFVAEGARVNARIAAQVIIVAGTVDGTLEC
                     SGRLEVLPSGRVSGEVFAPTLVVHEGATVDGDLKMQAAESAG"
BASE COUNT          496 a          920 c          938 g          577 t
ORIGIN      
        1 ttgtcctgcc cgttgctgtc ctcgtcgccg tctgactctt cgtcggggta accgtagtcg
       61 gccgccgtcg gagtctcctc ctgctcggag atggcgactt cgtggcgagt gcggccctcg
      121 atgatggcgt cggcaagccg agagacaatc aaccgaactg atcgaatcgc atcatcgttg
      181 gctgggatca cgtagtcgat cgggtccgga tcacagttgg tgtcaaccag cgcgatgatt
      241 gggatgccga gccggctggc ttccttgacc gcgagctctt cgcggcgcgg atcaacgacg
      301 acgatcgcgg ccgggcgctg ggtcagctct ttgaggccgc cgagcgagcg ctggagcttc
      361 tccagttcct gctccttggc agtcgcctcc tgcttcggaa gcaagttaag ttcgccggac
      421 atcgactgcc gttcgaggtt gcgcagatac tgcaagcgcg agcggatcgt gacaaagttc
      481 gtgagtgtac cgccgagcca gcggcggttg acaaagaaca tgccgcagcg gctggcttcc
      541 tgctcgaccg tcgcctgcgc ctgcttcttg gtaccgacga agacgatctt gccgttgcgc
      601 gcggcgatgt cagcgacgaa gtcctgggct tcgccgagca gccgggcggt ctgatggaga
      661 tcgatgacgt gaatgccgtt gcgctccccg aagatgtagg gacgcatttt gggattccag
      721 cgcttggtct ggtgcccgaa atggacacct gcctccagca gcgccttcat cgtgacctgg
      781 gtgtcttcgc gagacatcgt cgagaccaat gggttcctcc cgtgtttaca cttccgcgtg
      841 attcaacccg cactgccacc cgccggggcg ggaacacgga gcgacggtcc acacgcgtgc
      901 tgatgcatgc ccgcgatcgc gggccatcgg ggagtatacc atggccgcga gacggcctcg
      961 gtgttactga acgagagtca ggaaatcggc gacgatatag ccggtaccgg cctcgccggt
     1021 gaccggccac cagacgtaac cgtcggcttc ttctgccgga ccggtcgtcg tgaactcggt
     1081 tccgtcgagg acgaccgtta cgatctcacc atcgaccgac gcagtggcac gcatattcac
     1141 gcccgtcccg ccggtgttgg caaccgcgac gacggcgcct tcgtagatct cgcctgacgg
     1201 agcctcactt ggcgtcgtcg gcgtcggggc gccgcccgtg tttggcggag ttgtcgcgcc
     1261 aggctgggtc gtcaaggtaa tgcgcggcgt ttcggatccg ctggcctgat tcgtggcggt
     1321 gacggacggc tggatgactg ggatcgtcgt cgtagcggtc ccgcccgcgc cgagtgtgcc
     1381 gtcgtcatcg tcatcgccgt tcagaaaggg gatgatccag agcaacgcca ccagcagaat
     1441 caggatgacg ccgaggatcg gcaaaccgat ccggacataa tcggtccaat agcgctcttc
     1501 ggtgtagtaa tcgagctgtc gctcgcggcc acgttgctcg ggcgaacggc tgtcgggtgg
     1561 cgggatgtct tcgtcgttgg gatcgccagg atagccggac gaaccagaga ccggaaagat
     1621 aagctgacct ccaggtgtcg tgagcccggc gcggaaaccg ctgtcgccgg atgcgttcag
     1681 tgttgctgat tcagagttgc cgcccaccgg cttcaacggg atgtatggga cagggccgga
     1741 tgtctggctg gcttcccagt agttgcccat gccgcgttcg caatcgaggt cttcttcacc
     1801 gacgaggtgg ctttgctggg gcgaatacaa tagcgggttt cggcaccaac ccttgcgcca
     1861 gattggcgcg ggttcgtagt gcttacacga gccgcacttt cgccctgtca tcgccctcgc
     1921 cccccatcgc gcctgatacc acgccacacg ggcagtccgc ttccccttgc ggaagcccag
     1981 cgccaacaga ccagtacgga acgagagaca tcaacgatct gttggtcaga tcaaggttgg
     2041 ggtgcagtat aacacggctc ggcgtggcgt cagcggctat tcggggtctt cgtcgccggg
     2101 ggacgcaggg aggtcatcgt cgtcctcgcg cgccggcgtc gaaccgggcg tgaaactgcg
     2161 caggaacgag taagatccgg tcgcctgccc cggggcggcg gcttccggct ccgcttcggc
     2221 cacctgggtt ggcagcgggg tcagcggagt tggctcgact tgttcggcgt ccatccgcag
     2281 ttggccttcg tagacggcac cttcgacaac gatgagccgg ccggtctcaa cccgacctcg
     2341 caccatgcca gtcgagcgaa tctctagccg gccatgacag gtgatggcgc cgctcaacga
     2401 gccggcaacg acgatccccg ccgcttcgac ggtcgcgtca acctcggcgc cctcagtgac
     2461 atgcaggacc ccgtcacaca tgaccgttcc ggtgacgtgg ccatcaatcc ggagatcgtg
     2521 tgaggtcgac aacgttccgt cgatgacggt gttggcgtcg atgatcgtca tgccggtcgc
     2581 cgatctgtcc atcgtcgcgg aacccgccgt gctcgtgaag ctcattcgcc gtcactcctt
     2641 gatacagccc gatctgagag aggcactgtc agccggccga ttcagcagcc tgcatcttga
     2701 gatcgccatc gacggtcgct ccctcgtgga cgacgagcgt cggcgcgaat acttcaccgc
     2761 tgacgcgccc gctcggaagc acttcgagac ggccgctgca ttcgagtgtg ccatcgactg
     2821 tgcctgcgac gatgataacc tgcgcggcga ttctggcgtt gacgcgcgca ccttcggcaa
     2881 cgaagatctc gttgttggct gccagctccc cttcgacttc gccgaagatc t
//
LOCUS       DASUGJ010000260         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1568763, whole genome shotgun sequence.
ACCESSION   DASUGJ010000260 DASUGJ010000000
VERSION     DASUGJ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568763"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..2646)
                     /locus_tag="VFV93_05785"
     CDS             complement(76..2646)
                     /locus_tag="VFV93_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEX5164887.1"
                     /translation="MIPLQLKLKNFLSYRAETTLDLAPIRIACLAGDNGAGKSALLDA
                     MTWAIWGKTRASNDREVICIGETEMEVTFTFRLGERDYRIFRRRSFARGGSSGQHTLE
                     FYIAAPGGEDWLPISGDNVRETQEGIVGVLNMDYETFVNSAFILQGKADSFTQKPPSE
                     RKKILGDILNLQEYDELARLARDEEKSVRSRLDHDRTRINVLDEQLSERRSLVTNLEI
                     VSAQVTETSQKLDLAQDLAKSLALQLSAWERIRELLDGVRARAERERAALERLDRQLA
                     EAEQQRVAINDLLVQAGEIEHGAAEHLRWRELAAAFSATLVKVQQQVEIRNNAEREIA
                     AELGALQRGREGHVHAIRTAEQQLRGIEQDEARLVTLRREQADAIELLKQMPATRAKL
                     EEFRHEKSTLHADNGLLKSQMHEIKERLAQLQAGDATCPVCRTPLAPADRERIHAEWT
                     SEGTILGDRYRANKARMEEIDKALPILTEEEQRLIDADRQLAQLRGIITQIESGLTQR
                     EACLRQLDFASAEVERIDQQIATEAFAADARARLAAAQALLAELAYDANAHKEATANE
                     QQFAPFEARKRELDTARTRLEGVERERASIAGQQAERREAIETAEQEIAGFEAQLSTD
                     TDLRQRANDASDEVERLTRERNQHSGELAGIQRRLEHLDALQKERDDLDKETSALALD
                     HGALRELIDAFGRNGIQAMIVENVLPELEDEANALLRRMASSQLHVTFRSQRQALSSD
                     NVIETLDILIRDEYGERPYALYSGGEAFRVNFAVRVALSKLLARRAGANINMLVIDEG
                     FGTQDTSGRDGLIEALHSVEQDFQTILVITHIGEIRELFPTRIDIVKTETGSKIAVNA
                     "
BASE COUNT          395 a          899 c          945 g          546 t
ORIGIN      
        1 gatgctagcc gaccggtgac aggacggcaa attcacgctg gtcgactact caggacgaca
       61 aggggaaaga ctccactagg cattcacagc gatctttgag ccggtttcgg tcttgacgat
      121 atcgatccgg gttgggaaga gctcgcggat ctcgccgatg tgggtgatga cgaggatcgt
      181 ctggaaatcc tgctcgaccg agtgaagggc ttcgatcagg ccgtcccggc cgctggtgtc
      241 ctgggtgccg aagccctcgt cgatgacgag catgttgatg ttcgcgccgg cccgccgtgc
      301 cagcagcttc gagagggcga cccggacggc gaagttgacc cggaacgcct cgccgccgga
      361 gtagagcgcg tagggccgct caccatactc gtcgcggatc aggatgtcga gcgtctcaat
      421 cacgttgtcg ctggacaggg cctgtcgctg cgagcggaag gtgacgtgta actggctgga
      481 cgccatccgc cgtagcagcg cgttggcttc gtcctcgagt tccggcagga cgttctccac
      541 gatcatcgcc tggatgccgt tgcgtccgaa ggcgtcgatc agctcacgca gcgcgccgtg
      601 gtcgagcgcc agcgcgctgg tctccttgtc gaggtcgtcg cgctccttct gaagtgcgtc
      661 gaggtgttcc agccggcgct ggataccggc cagctcgccg gagtgttgat tgcgctcgcg
      721 cgtcagccgc tcgacttcgt cggatgcgtc gttggcccgc tgccggaggt ccgtatcggt
      781 tgatagttgt gcctcgaagc cggcgatctc ctgctcggcg gtctcgatcg cctcgcgccg
      841 ttcggcctgc tgaccggcga tcgacgcacg ttcccgctcg acgccttcca gccgggtgcg
      901 ggccgtgtcc agctcccgct tgcgtgcctc gaacggggcg aactgctgct cgttggcggt
      961 tgcttcctta tgggcgttcg cgtcataggc cagttcggcc agcagcgcct gggccgctgc
     1021 cagccgcgcg cgggcatcgg cggcgaacgc ctcggtcgca atctgctggt cgatgcgttc
     1081 gacctcggcg ctggcgaagt ccagttgtcg caggcacgcc tcccgctggg tcagcccgga
     1141 ctcgatctgg gtgatgatgc cgcgtaactg cgccagttgg cggtcggcgt cgatcagccg
     1201 ctgctcttcc tcggtgagga tcggtagtgc cttgtcgatc tcctccatgc gggccttgtt
     1261 ggcacggtag cggtcgccga ggatcgtccc ttcggaggtc cattcggcgt gtattcgctc
     1321 gcggtcggcc ggcgccagcg gtgtccggca gaccgggcag gtcgcgtcgc cggcctgaag
     1381 ctgcgccaac cgctccttga tctcgtgcat ctgcgacttc agtagcccgt tgtcggcgtg
     1441 cagcgtcgac ttctcgtggc ggaactcttc gagcttggcg cgggtggccg gcatttgctt
     1501 cagcagctcg atggcgtcgg cctgctcgcg gcggagcgtg acgagtcggg cctcgtcctg
     1561 ctcgatgccg cgcagctgct gctcggccgt ccgtatcgcg tgcacgtgtc cctcgcgtcc
     1621 acgctggagc gcgcccaact cggctgcgat ctcccgttcg gcgttgttgc ggatctcgac
     1681 ctgctgctgg accttgacga gcgttgccga gaacgcggcg gccagctcgc gccagcgcag
     1741 atgctcagcc gcgccgtgtt cgatctctcc ggcctgcacc agcagatcgt tgatggcaac
     1801 gcgctgctgc tccgcctcgg ccagttgccg gtcgagccgt tcgagggcgg cccgctcacg
     1861 ctcggcacgg gcgcggacgc cgtccagtag ctcgcggatg cgctcccagg cgctgagctg
     1921 gagcgccagc gacttcgcca gatcctgggc cagatcgagc ttctggctcg tctcggtcac
     1981 ctgggcgctg acgatctcga ggttggtgac cagcgagcgg cgctcggaga gttgctcgtc
     2041 gaggacgttg atgcgggtcc ggtcgtggtc gagccggctg cggacggact tctcctcgtc
     2101 acgggccagg cgcgccagct cgtcgtactc ctgcaggttg aggatgtcgc cgaggatctt
     2161 cttgcgctcg ctgggcggct tctgagtgaa ggagtcggcc ttcccctgca ggatgaaggc
     2221 cgagttgacg aaggtctcgt agtccatgtt gaggaccccg acgatcccct cctgcgtctc
     2281 gcggacgttg tcgccggaga tcggcagcca gtcttccccg cccggcgcgg cgatatagaa
     2341 ctccagcgtg tgctggccgc ttgagccacc ccgcgcgaac gagcggcgcc ggaagatgcg
     2401 atagtcgcgc tcgccgagcc ggaaagtgaa ggtgacttcc atctccgtct caccgatgca
     2461 gatcacctcg cggtcgttgc tcgcgcgggt cttgccccag atcgcccagg tcatcgcgtc
     2521 cagcagggcc gacttcccgg cgccgttgtc accggccagg caggcgatcc ggattggcgc
     2581 gaggtcgagc gtcgtctccg cccggtagga gaggaaattc ttgagcttga gttggagagg
     2641 aatcatgccg cacaatccag actggccccg cgcgagacac gcggcagcag aacacgcatc
     2701 attgcgccgg accccaagta tactgcgtca gaggccgcat tcgttcgcgg aaagacatct
     2761 gtattcagag gtaagaggca tcgga
//
LOCUS       DASUGJ010000261         7345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_734153, whole genome shotgun sequence.
ACCESSION   DASUGJ010000261 DASUGJ010000000
VERSION     DASUGJ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734153"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1457)
                     /locus_tag="VFV93_05790"
     CDS             complement(42..1457)
                     /locus_tag="VFV93_05790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX5164888.1"
                     /translation="MSGATKQGTTITMPKLGESVTEGTLGAWLKRVGDAVDKYEPMVE
                     VVTDKVTAEIPAPVSGTIVEIIGGEGDVIPVGGLICVIDEGNGASQTVSPPVDQQPQA
                     SMPEADSTTPTVDTLPTSGPSGSGTVIDLTEARSRHDEAELLRARSSPIVRRLAEEHE
                     IDISAMHGSGIGGRVTKADMLAEIDARASQPQHPAAAAPQLQPVAAAAPAPAPTVAPP
                     MAAPSIAQPMPAQQAAVVQLMPGDEVIEASVMRRQIADHMVRSAYTAPHVTVWMEADM
                     SNIVRVRDRYKEEFKTRESASLTFVPFVLRVLTQALREHPNINAAWDNGRIIRRKAIN
                     IGIAVAMEDGLIVPVIHNADEKNIVGLARSVNDLANRARENRLQSDEVTGGTFTLNNP
                     GTLGSLFSTPIIVQPQAAILSMEAVVKKPVVIDDAIAIRPMMNLSLSLDHRILDGLSA
                     TRFLATVKAGLETFPADASIY"
     gene            complement(1569..2555)
                     /locus_tag="VFV93_05795"
     CDS             complement(1569..2555)
                     /locus_tag="VFV93_05795"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5164889.1"
                     /translation="MATKLLIEAIREGLTQEMEADERVFVLGEDVGKRGGVFRVTEGL
                     LDKFGEMRVIDSPLAESSIVGVAIGAAANGMLPVAEIQFLDFIHPAMDQIMNEAAKFR
                     YRSGGDFGCPIVIRSPYGGGIHGALYHSQSIEALFTHTPGLKVLAPVDPYDAKGLLRS
                     AIHDPDPVLFLEHKRAYRAVRAEVPDEPYDVPIGKARVVREGSDMTIISWGWYLQESL
                     QAAEAYAQEQNASIEVIDLRSLRPLDRDTILESVMKTGKVLIVHEANKFGGLGGEISA
                     IIAEEAFAYLDGPIVRLAGPEVPAMPYAQPLEKAFLHDKDDIRAAIEKLAQY"
     gene            complement(2651..3685)
                     /locus_tag="VFV93_05800"
     CDS             complement(2651..3685)
                     /locus_tag="VFV93_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX5164890.1"
                     /translation="MGRTEPGGTATATRDGVEGLSHDDMRQMYETMLLARQLDERMWL
                     MNRAGRAPFVISVQGHEGAQAGVGFALDTSKDWLLPYYRDLALVLHFGMTPRDMMMSF
                     LARKGEPNSRAKQMPGHYGSRRHRIITSSSPVGTQFAQAPGVALAARMRGEDTVVVTS
                     IGEGGTAQGDVHEGLNWASIYKLGVIFFVQNNHYAISVPQHKEMAIKNVSERAAAYGM
                     AGVTVDGADAIDVYVKAREAVERARRGDGPTLFEAKVNRMTPHSSDDDDRFYRSREEV
                     ASYRERDPIVVTGQRLRDLGALTDEQEQEIRNRVKAAVNDATDFAEAAPPPDPEEAFT
                     DVYAPKGARV"
     gene            complement(3915..5309)
                     /gene="lpdA"
                     /locus_tag="VFV93_05805"
     CDS             complement(3915..5309)
                     /gene="lpdA"
                     /locus_tag="VFV93_05805"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5164891.1"
                     /translation="MAQEQGYDVAFLGGGTGGYVAAIRASQLGLRAVVIEKDLVGGTC
                     VHRGCIPAKALLKSASVATLVKEAKDFGVTPGDISLDYTTAMSRARKVVDQNYKGIQF
                     LFKKHKIDVVEGWGTITGPGAISVEKDGKTTEVRAGSIVIDTGSTPRPIDGIEIDGKR
                     VFTSDHATTQPYLPKRVIVRGGGATGLEFASVYREFDCEVTLVGRVAPNEDEEVQKDL
                     ARSFQRQGIRVLANVRPTADDFTISDKGVTLKVSVDGKDETIEADSLLVAIGRYGNTE
                     GFGLEKIGVKVEKGYIVVDDHMRTNVENVYAIGDVTGLQQLAHTAMHQGVVAVETIAG
                     HKPEVLDYNKIPWVTYCHPEVGSVGLTEKKARDAGHNVKTGKFPLSANGKARVENDTT
                     GFAKMVVDADTDQILGIHLLGGHATELISVGGLAMLFEATAWELGITTFPHPTISEVL
                     HEAALDVDGAALHF"
     gene            5644..5931
                     /locus_tag="VFV93_05810"
     CDS             5644..5931
                     /locus_tag="VFV93_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164892.1"
                     /translation="MTITRIGTVTPLETWRRMAAKAGKEQLRAYRINGDPRYWVVSSS
                     SDPTVAYEVTAHDGDLICSCRASEFLPYCKHRALVMRELGALETWTEAIAA"
     gene            complement(6148..>7345)
                     /locus_tag="VFV93_05815"
     CDS             complement(6148..>7345)
                     /locus_tag="VFV93_05815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX5164893.1"
                     /translation="LRVDGLFEHVGMDYLTSYTAAEIIRDGDIKDLYDTRVQWEYQRP
                     VIADHGVEWGDRIMHPYIAPPLLGVLSIPLLALGPAVAYAAWALLNTAAVALGIWLVA
                     RRFAIEWTAPLTVLAGSLPLFMVLMLGQVEGLLFLAFVVFLIEIRDGREVRAGLALAV
                     FAIKPPLMLAPMLYLVVTGRRRATWTTIGATAAQAIGSMLLVGPGGVRDYIALSQRLS
                     GPDGTTVTNVWGMVNLRSIVVRAVPADEPMLTNLMIVLLTGITLAVACWFWRRAATEP
                     VMLPSLALLAVTTVLTSYHALYHTATLALLGVILLIAYAHSQREPSRAEWLTIGSWAM
                     FSLLPLLAFIVVQTSQVPAAIGVAGVLVFWAVAAGTVAQQVAATEAATRPSRAELQPW
                     YVIERD"
BASE COUNT         1236 a         2412 c         2372 g         1325 t
ORIGIN      
        1 cttcgctgcg gctcaggacg acaaggggcc gcgaattgcc cctagtagat cgaggcgtcg
       61 gccgggaacg tctccaatcc tgccttgacc gtcgcgagga agcgggtggc cgagaggccg
      121 tcgaggatgc gatggtcgag cgagagtgag agattcatca tcggccggat cgcgatcgcg
      181 tcgtcaatga caaccggctt cttgacgacg gcctccatcg acaggatcgc cgcctgcggc
      241 tggacgatga tcggcgtcga gaagagcgag ccgagcgtgc cggggttgtt gagtgtgaac
      301 gtcccgccgg tcacctcgtc cgactggagc cggttctcac gcgcgcgatt ggcgaggtcg
      361 ttgaccgagc gcgccaggcc gacgatgttc ttctcatcgg cgttgtgaat caccggaacg
      421 atcagcccgt cttccatggc gacggcgatg ccgatgttga tcgccttgcg gcggatgatc
      481 cggccgttgt cccaggcggc gttgatgttg ggatgttcgc gtagtgcctg ggtcagcact
      541 cgcaggacga acggcacgaa ggtcaggctg gcgctctcgc gggtcttgaa ctcttccttg
      601 taacgatccc ggacacggac gatgttgctc atgtccgctt ccatccagac cgtcacgtgc
      661 ggagccgtgt aggccgaacg gaccatgtgg tcggcgatct gccgccgcat gacgctcgcc
      721 tcgatcacct cgtcgccagg catcagctgg actaccgccg cctgctgtgc cggcatcggc
      781 tgggcgatgc tcggtgcggc catcggtggc gcgacggtcg gggccggcgc tggtgcggcc
      841 gccgcgacag gctgtagctg cggcgcagct gcggccggat gctgcggctg gctcgcgcgc
      901 gcgtcgattt cggccagcat gtcggccttg gtgacgcggc cgccgatgcc actgccgtgc
      961 atcgcgctga tgtcgatctc gtgttcttcg gccagcctgc ggacgatcgg cgacgatcga
     1021 gcgcgcagca actcggcctc gtcatgccgc gagcgcgctt cggtcagatc aatgaccgtg
     1081 cctgatccgc tcggaccgct ggtcggaagc gtatctaccg tcggcgtcgt cgagtcggct
     1141 tccggcatcg acgcctgcgg ctgctgatcg acaggcggcg agacggtctg cgacgcgccg
     1201 ttgccttcgt caatcacgca gatcagcccg ccaaccggga tcacgtcgcc ctcgccgccg
     1261 atgatctcga cgatcgtccc ggacaccggc gccgggatct cggccgtgac cttgtcagtg
     1321 acgacctcca ccatcggctc gtacttgtca accgcgtcac caactcgctt cagccaggcg
     1381 cccagcgtcc cctcggtaac gctctcgccc agcttgggca tcgtgatcgt cgtgccctgc
     1441 ttcgttgctc cagacatgct gcctcctgtg atcagtgctg agtgatgggt tctgagggct
     1501 gagtctcgat cgcgtaggcg cgatggccgc agcctcgccc ggtatcgtca ggactcagca
     1561 ctcagcactc aatactgtgc gagcttctcg atcgcggcgc ggatgtcgtc cttgtcgtgc
     1621 aggaatgcct tctcgagtgg ttgcgcgtag ggcatggccg gcacttcggg acctgccagc
     1681 cggacgatcg ggccgtcaag ataggcgaac gcctcctcgg cgatgatggc cgagatctca
     1741 ccgcccaacc ctccgaactt gttggcctcg tggacgatca gcaccttgcc ggtcttcatg
     1801 accgactcga ggatcgtgtc cctgtcgagt ggccgcaacg agcgcaggtc gatgacctcg
     1861 atcgaagcgt tctgctcttg cgcgtaggct tcggccgcct ggagcgactc ctgcaggtac
     1921 caaccccacg agatgatcgt catgtcgctg ccctcgcgga cgacccgcgc cttgccgatc
     1981 ggcacgtcgt atggctcgtc cggcacctcg gcacggacgg cgcggtaggc gcgcttgtgt
     2041 tccaggaaga ggaccggatc ggggtcgtgg atcgcgctgc gcaacaaccc cttggcgtcg
     2101 tacggatcga ccggcgctag caccttcagg cctggcgtgt gagtgaatag tgcctcgatc
     2161 gactgcgagt ggtagagcgc gccgtggata ccgccgccgt acggtgagcg gatgacgatc
     2221 gggcagccga aatcgccgcc ggagcgatag cgaaacttgg cagcctcgtt catgatctgg
     2281 tccatggccg ggtggatgaa gtcgaggaat tgaatctcag cgaccggcag catcccgttg
     2341 gccgccgcgc cgatcgcaac gccgacgatc gatgactcgg ccagcggcga gtcgatgacg
     2401 cgcatctcgc cgaacttgtc cagcagccct tcggtcacgc ggaagacgcc gccgcgcttg
     2461 ccaacatcct cccccaggac gaagacacgc tcgtcggcct ccatctcctg ggtcaatccc
     2521 tcgcggatcg cctcaatcag cagcttcgtt gccacggatg gttcctttcg tgtcgtgatg
     2581 cgtgacgcgt gacgcggagt ggcttggtcg ttgagtcgcg tcacgcatca cgcgtcacgc
     2641 gtcacgaacc tcagactcgc gcgcccttgg gagcgtagac gtcggtgaag gcttcctcgg
     2701 gatctggcgg aggagcggct tccgcgaaat cggtggcgtc gttgacggcc gccttgacac
     2761 ggttgcggat ctcctgctcc tgctcgtcag tcagcgcgcc gaggtcgcgc aggcgctggc
     2821 cggtgacgac gatcggatcg cgctcgcggt aggaggcgac ctcctcgcgg ctgcggtaga
     2881 agcggtcatc gtcatcggag gagtgcggcg tcatccggtt gaccttggcc tcgaagaggg
     2941 tcggtccgtc gccacgacga gctcgttcga ccgcctcgcg ggccttgacg tagacgtcga
     3001 tcgcgtcggc gccatcgacc gtcacgccgg ccatcccgta ggcggctgct cgctcggaga
     3061 cgttcttgat cgccatttcc ttatgctggg gaaccgagat cgcgtagtgg ttattctgga
     3121 cgaagaagat cacaccgagc ttgtagatgc tggcccagtt cagcccctcg tggacgtcgc
     3181 cctgggcggt cccaccttcg ccaatgctgg tgacgacgac cgtatcctcg ccacgcatcc
     3241 gcgccgccag cgccacaccg ggcgcctggg cgaactgggt gccgaccggc gaggaactgg
     3301 tgatgatccg gtgacggcgc gaaccgtagt ggcccggcat ctgcttcgcg cggctgttcg
     3361 gctcgccttt gcgggccagg aacgacatca tcatgtcgcg cggggtcatc ccgaaatgga
     3421 ggacgagggc gagatcgcga tagtagggga gcagccagtc cttgctggtg tcgagcgcaa
     3481 agccaacgcc ggcctgggcg ccctcgtggc cctggacgga gataacgaac ggcgctcgac
     3541 cggcccggtt catcagccac atccgctcgt cgagctggcg agcgagcagc atcgtctcgt
     3601 acatctgccg catgtcgtcg tgcgagaggc cctctacgcc gtcgcgagta gccgtcgccg
     3661 ttccacctgg ttcagttcgc cccatctggc gcctccttcg ccgtcccggc ccgtcgttgg
     3721 accggtccat cgtctgagcg tgagcacggt gcggggacat ctcgtcatgc gcgtgacgcg
     3781 atgcgcaatg gcggtccgcc atcattgtcc tgcgaagcgg tcccgactca gaaccgcctc
     3841 gcgttacccg tcatcgcctc cttcgtttcc atcctcgggc gcacaacctg taccgcccac
     3901 tacctgatac gcaattagaa atgcagcgcg gcgccgtcga catcgagggc ggcctcgtgc
     3961 aggacctcgg agatcgtcgg atgcgggaag gtcgtgattc ccagctccca ggcagtcgcc
     4021 tcgaagagca tcgccaaccc accgacgctg atcagctccg tcgcatggcc acccagcagg
     4081 tgaatgccga ggatctggtc ggtgtcggca tcgacgacca tcttggcgaa gccggtcgtg
     4141 tcgttctcga cgcgggcctt gccgttggcg gagagcggga acttgccggt cttgacattg
     4201 tggccggcgt cgcgggcctt cttctcggtc agtccgaccg agccgacctc cgggtggcag
     4261 taggtcaccc acggaatctt gttgtagtcc agcacctccg gcttgtggcc ggcgatcgtc
     4321 tcgacggcga cgacgccctg gtgcatggcc gtgtgcgcca gctgctggag gccggtcaca
     4381 tcgccgatgg cgtagacgtt ctcgacgttg gtgcgcatgt ggtcatcaac gacgatgtag
     4441 cccttctcga ccttgacgcc gatcttctcc agcccgaacc cttcagtgtt gccgtagcgg
     4501 ccaatcgcga ccagcagcga gtcggcttcg atcgtctcgt ccttgccgtc gacactgacc
     4561 ttgagcgtca cgcccttgtc gctgatcgtg aagtcgtccg ccgtcggccg gacgttggcc
     4621 agcacgcgta taccctgccg ctggaacgag cgggcgaggt ctttctggac ttcctcatcc
     4681 tcgttcggcg cgacacggcc caccaatgtg acctcgcagt cgaactcgcg gtagacgctg
     4741 gcgaactcca gcccggtcgc gccgccgccg cgcacgatca cgcgcttcgg caggtacggc
     4801 tgggtcgtcg catggtcgga cgtgaagacg cgcttgccat cgatctcgat cccgtcgatc
     4861 ggacgcggcg tcgagccggt gtcgatgacg atgctgccgg cgcgcacctc ggtcgtcttg
     4921 ccgtccttct cgacgctgat cgcgccgggg ccagtaatcg tgccccagcc ctcgacgaca
     4981 tcgatcttgt gcttcttgaa gaggaactgg atgcccttgt agttctgatc gacgaccttg
     5041 cgggcgcggg acatcgccgt cgtgtagtca agcgagatgt cgccaggcgt gacgccaaag
     5101 tctttcgctt ctttgacgag cgtcgcgacc gatgcagatt tgaggagcgc cttggcgggg
     5161 atacagccgc gatggacaca ggtgccgccg acgagatcct tttcgatgac gaccgccctc
     5221 agtccgagct gcgacgccct gatcgcggcg acgtatccgc cggtgccgcc accgaggaac
     5281 gcgacatcat acccttgctc ctgcgccata ccgccttgac tctcccgtcc cagcttgcgg
     5341 ctgtccattg ccgcgacaat tggctccgct cacaacgcgg cccaacccag actgtagccc
     5401 cggttgacgg cgcaagacgc gacgcggcgc tttcagaatc gagccgcaaa cagtctcgca
     5461 gcacgcatgc cagagccata ttcactgaca aacagtatat cagggcatac gacatcaccg
     5521 acgcagtttc acggattgac gcgacgattt tccgcgtcag aatcgcgttt ctgacttgct
     5581 gtcacagccg gtgagcggta taatagaaca gatgtacgac gttgtacacg aaagcgggat
     5641 gagatgacga taacgcgaat tgggacggtg accccgctcg aaacctggcg acggatggcg
     5701 gccaaggccg gtaaggagca actgcgcgcc taccggatca acggcgaccc gcgctactgg
     5761 gtcgtctcga gcagcagcga cccgacggtg gcctacgagg tcaccgccca cgatggcgac
     5821 ctgatctgct cgtgccgcgc gagcgagttc cttccctact gcaaacaccg cgcgctggtc
     5881 atgcgtgaac tcggcgcgct cgaaacctgg accgaggcga ttgccgcctg aggcgacccg
     5941 cggtgaaccc gccacacgtg taggggccgt gcctccgtgc cggcccgcgc cgtgaggcgc
     6001 ccggtttacg gcaagtgcgt tccccagcac gtgcgctagt gtattcaccc gtacgcgggt
     6061 acgaaggcgc cgcccgccca tcgcgggccg gcacggaagc acggccccta ccgaggacgg
     6121 cttttccacg cgtgcactgt atgtgcttca gtcacgttcg atgacgtacc agggctgtag
     6181 ctcggcgcgc gatggtcggg tggcggcttc ggtcgcggcc acctgctggg caactgtgcc
     6241 ggccgcgacg gcccagaaga cgaggacgcc agcgacgccg atggcggccg ggacctggga
     6301 tgtctggacg acgatgaagg ccagcaaagg caggagcgag aacatcgccc agctaccgat
     6361 cgtcagccat tcggcccgtg acggctcgcg ctgactatgc gcgtacgcga tcagaaggat
     6421 cacccccagc agcgccagtg tcgccgtgtg atatagcgcg tggtacgaag tcaagacggt
     6481 cgtcaccgct agcagcgcca ggctcggcag catgaccggc tcggttgctg cccgtcgcca
     6541 gaaccagcag gcaaccgcca gcgtgatgcc agtcagcagg acgatcatca ggttcgtcag
     6601 catcggctcg tcggccggaa cggctcgcac gacgatcgag cgcaggttga ccatgcccca
     6661 gacattggtg acggtcgtcc cgtccgggcc agagagacgc tgcgaaagcg cgatgtagtc
     6721 acggacgcca cctggcccaa ccagcagcat cgagccaatc gcctgggcgg ccgtcgcccc
     6781 aattgtcgtc caggtcgcac ggcgccggcc tgtcacgacc aggtagagca tcggcgccag
     6841 catcagcggc ggcttgatcg cgaagacagc gagggccagc ccggcacgaa cctcgcggcc
     6901 gtcccgaatc tcgatcagga agacgacgaa cgcgagaaac agcagccctt cgacctgacc
     6961 gagcatcaga accatgaaga gcggtagtga gccggccaga accgtcaacg gagctgtcca
     7021 ttcgatcgcg aaccgcctgg cgaccagcca gataccgagc gcgacggccg ctgtgttcag
     7081 caacgcccag gcggcatacg ctactgccgg cccgagtgcc aggagcggga tcgagaggac
     7141 accgagcagc ggcggcgcga tgtaggggtg catgatcctg tccccccact cgacgccgtg
     7201 gtcggcgatg acgggccgct gatattccca ttggacgcgg gtgtcgtaga gatccttgat
     7261 atcgccgtca cggatgatct cggcggcggt gtagctggtg aggtaatcca tcccgacgtg
     7321 ctcgaagaga ccatcgacac gcaac
//
LOCUS       DASUGJ010000262         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_456482, whole genome shotgun sequence.
ACCESSION   DASUGJ010000262 DASUGJ010000000
VERSION     DASUGJ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456482"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /gene="dnaK"
                     /locus_tag="VFV93_05820"
     CDS             complement(<1..1341)
                     /gene="dnaK"
                     /locus_tag="VFV93_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX5164894.1"
                     /translation="MAKVIGMDLGTTNSVVAVMEGGEPTVIPNAEGNRITPSVVAVSR
                     SGERMVGQVAKRQAITNPENTIYSIKRLMGRKFDDPEVQKIISLVPYKIVRASNGDAH
                     VELGGRVYSPPEISAMILQKLKSDAEAYLGEKVNEVVVTVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKKGDEEIAVYDLGGGTFDISILELGEGTFQVKSTNG
                     DTLLGGDDFDQRVIEWILDEFKKNEGVDLRADRAAMQRLKEAAERAKIELSSVQQTEI
                     NLPYVTADASGPKHLLLTLTRSKLEQLVMDLVEKTMGPCRQALQDAGISGSQVDEVVL
                     VGGQTRMPLVQEKVKQFFGKEPHKGVNPDEVVAIGAAIQAGVLKGEVKDVLLLDVTPL
                     TLGIETLGGVMTPLIPRNTTIPTSKSQTFSTATDSQPSVEIHVLQGERTMAPDNK"
     gene            complement(1434..1994)
                     /locus_tag="VFV93_05825"
     CDS             complement(1434..1994)
                     /locus_tag="VFV93_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5164895.1"
                     /translation="MSKDQHGRDGAEETDVTSGHPDPAVQTEEPAEAIRAQLEEAQAE
                     ARENFNKYQRALADFQNYKRRTEEQRADYRRDANAALVINILPAIDDLDRALASVDAK
                     LAGLQWIEGIRQIQRKFQGALTALDVTEIEADGVDFDPRLHEAVGHVPGEGGKVVQVL
                     QKGYTVGDRVVRPAMVMVGNGETDGQ"
     gene            complement(2083..>2510)
                     /locus_tag="VFV93_05830"
     CDS             complement(2083..>2510)
                     /locus_tag="VFV93_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HEX5164896.1"
                     /translation="ADSTALDRSIATVVADILAEEDAAAFAEARVEGLRNVLSQPEFS
                     RSEKLLDLMEAVDERNLPKAIPAESVADEGVTVIIGTENPNDSMRECSIVITSYGTPE
                     GHKGAIAVLGPTRMHYPRAIATVRYMGRVMTDVLTKIYS"
BASE COUNT          351 a          860 c          763 g          536 t
ORIGIN      
        1 cttgttgtcc ggcgccatcg tccgctcgcc ctggaggacg tggatctcga cgctcggctg
       61 gctgtccgtc gccgtgctga aggtctggct cttcgaggtc gggatcgtcg tgttgcgcgg
      121 gatcagcggc gtcatcacgc cacccagcgt ctcgatgccg agcgtcagtg gcgtcacatc
      181 gagcagcagg acatccttca cctcgccctt gagcacgccc gcctggatcg cggcgccgat
      241 cgcgacgacc tcatccgggt tgacgccctt gtgcggctcc ttgccgaaga attgcttcac
      301 cttctcctgc acgagcggca tccgcgtctg gccgccgacg aggacgacct cgtcaacctg
      361 gctgccggag atgcccgcgt cctgcaacgc ctgtcgacat ggacccatcg tcttctcgac
      421 gaggtccatc accaattgct ccagcttgct ccgtgtcaac gtcagcagca ggtgcttcgg
      481 tccgctggcg tccgccgtga cgtacggcag gttgatctcg gtctgctgga cgctggagag
      541 ctcgatcttg gcgcgctccg ccgcctcttt cagccgctgc atcgccgccc ggtcggcgcg
      601 caggtcaacg ccctcgttct tcttgaattc gtcgaggatc cactcgatca cgcgctggtc
      661 gaagtcgtcg ccgcccagca gcgtgtcgcc gttagtcgac ttcacctgga aggtgccctc
      721 gcccagctcg aggatcgaga tgtcgaaggt gccgccgccg aggtcgtaga ccgcgatctc
      781 ctcgtcgccc ttcttgtcga ggccgtaggc cagcgccgcc gccgtcggct cgttgatgat
      841 gcgcagcact tcgaggccgg cgatcttgcc cgcgtccttt gtcgcctgtc gctgcgagtc
      901 gttgaaataa gcggggacgg tgacgacgac ttcgttcacc ttctcgccca gatacgcctc
      961 ggcgtcggac ttcagcttct gcaggatcat cgccgagatc tcgggcggcg aatagacgcg
     1021 gccgcccaat tcgacgtgcg cgtcgccgtt cgacgcccgc acgattttgt acggtaccaa
     1081 cgagatgatc ttttgcacct cggggtcgtc gaacttgcgg cccatcagcc gcttgatcga
     1141 atagatcgtg ttctccgggt tggtgatcgc ctggcgcttt gcgacctggc cgaccatgcg
     1201 ctcgccggaa cgtgacacgg cgacgacgga cggtgtgatc cggttgccct cggcattcgg
     1261 gatcacggtc ggctcgccac cctccatcac agcgacaacg ctgttggtcg tgcccaggtc
     1321 catgccaatt actttagcca ttggtgttgt gctcctcgct tctctcgtcg ccggcagtgg
     1381 gcaatcggca gtagacagtg ggggacgaac cccgattgcc cattacccac cgcctactgc
     1441 ccgtctgtct ccccgttgcc gaccatgacc atcgccgggc gcaccacgcg gtcgccgacg
     1501 gtgtagccct tttgcagcac ttggacgact ttgccccctt cgccggggac gtgaccgacc
     1561 gcctcatgca ggcgcgggtc gaagtcgacg ccgtcagcct ctatctccgt tacgtcgagc
     1621 gccgtcagtg cgccctggaa tttgcgttga atctggcgga tcccctcgat ccactgcagc
     1681 ccggccagct tggcatcgac gctggcaagc gcgcggtcga ggtcgtcgat cgccgggagg
     1741 atgttgatca ccagcgccgc gttggcatcg cgccggtagt cggcccgctg ctcctccgtg
     1801 cgccgcttat agttctggaa gtcggccagc gcgcgctggt atttgttaaa gttctcccgc
     1861 gcttcggcct gtgcttcctc gagctgagcg cgaatcgcct cggccggctc ttcggtctgg
     1921 acggccggat caggatgacc cgaagtgaca tccgtctctt cggcgccatc gcgcccgtgc
     1981 tggtctttgc tcattcgctt tcctcgccgt tctgcccctg ttgccatact ctgcccctcg
     2041 gctgttgatg tagcgttgtg tccgccgccg tcgtgtccgg cgttagctgt agatcttcgt
     2101 cagaacatcg gtcatcaccc ggcccatgta gcgcaccgtc gcgatcgcgc ggggatagtg
     2161 catccgcgtc ggtccgagca cggcgatcgc gcccttatga ccctccggcg tgccgtagct
     2221 ggtgatgacg atgctgcatt cgcgcatcga atcgttcgga ttttctgtgc cgatgatgac
     2281 ggtgacgccc tcgtcggcga cgctctccgc cgggatcgcc ttgggcagat tgcgctcgtc
     2341 caccgcctcc atcaggtcca gcagcttttc ggagcgactg aactccggct gggagagcac
     2401 attccgcagt ccttcaaccc gggcctcggc aaacgcggcc gcgtcctcct cggcgaggat
     2461 gtcggcgacg accgtcgcga tcgagcgatc gagcgcggtc gaatcggccg
//
LOCUS       DASUGJ010000263         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1984681, whole genome shotgun sequence.
ACCESSION   DASUGJ010000263 DASUGJ010000000
VERSION     DASUGJ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984681"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFV93_05835"
     CDS             complement(<1..700)
                     /locus_tag="VFV93_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5164897.1"
                     /translation="MTTPGTKQNAIKRVMLLMSPATYRAAAFYEAAKGLGIETVSVVD
                     MPDHLQEYWGQSHGVDFSDAGRATTTIVELAKSDRVDALLSVDDSATELAALAGSLLG
                     VPHNAPDAAVAARDKFKMRSMLAAGGVRCPGFRTFPAGADPRTYANEVTYPSVVKPTR
                     LSGSRGVIRANDADELIAAVNRLRAILTSDGTDLNTAGILIEDYMPGEEVAVEGLLTD
                     GELHVLTIFDKPDPL"
     gene            1187..>4014
                     /gene="ileS"
                     /locus_tag="VFV93_05840"
     CDS             1187..>4014
                     /gene="ileS"
                     /locus_tag="VFV93_05840"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX5164898.1"
                     /translation="MAKALKAFREVDTRADFPAMERERLRHWYEDGIVERYLTKNDDS
                     ETRWSFLDGPITANNPMGVHHAWGRTYKDLYIRYHTMLGHKQRYQNGFDCQGLWVEVE
                     VEKELGLATKRDIEEFGIDRFVELCKERVLKYAGIQTEQTKRLGNFMDWDNSYYTMSD
                     VNNYTIWDFLKTCNEKGWIYKGDDVMTWCPRCGTGISEMELNEGYQDRTHRSVYAYFP
                     LEDEDASLLVWTTTPWTLTSNVAAAVHPELTYLKVRQGEHVFYLAKDAAQHALKGEFD
                     TLAEVTGADLVGRRYRGPFDELPANADVEHRVIAWDDVAADEGTGIVHIAPGCGKEDY
                     KLSKEQDLQVVAPIDELGNYVAGFDWLSGMYVHEVAMPIVRNLREKGLLYRDHEYTHR
                     YPTCWRCKTDLVFRLVDEWYIGVDGMRERMMELTREIRWIPEFGEEREIDWLRNMDDW
                     MISKKRYWGLALPIYECPDCGTFEVIGSKEELGERAVEGWEEFEGHTPHRPWIDSVKI
                     QCKECGQIVSRIKDVGNPWLDAGIVPFSTMGYTEDREEWRKWFPADFITESFPGQFRN
                     WFYSMLAQSTALTGEAPYRTVLGFALLRDEHGKEMHKSAGNAIWFDDAADVMGSDVMR
                     WLYCQHNPAQNLNFGYHIGDEMRRRFIIPLWNSYAFFSNYAALDGFDPTDPSNDVPLA
                     ERTMLDRWIISRLQGLIGDVRAALDDYDQVTATRAFERFVVEELSNWYIRRNRRRFWK
                     TEADRDKAAAYLTLYEVLVTLSGLLAPFVPFLADEMYDNLVLSVEPDAAPSVHLTDYP
                     VADGAKVDAALDRDMAALLQAVNLGRAARNKANVKVRQPLPEVRVWARDEATLAAVER
                     MQDQLLDELNVKGLGRIDDPAQYATYVIRPNLSLLGPKYGKRLGELRGLLAGADPATV
                     AATIRSGDELALGEFRLTPDEVLVDVKER"
BASE COUNT          741 a         1166 c         1409 g          698 t
ORIGIN      
        1 ccagcggatc cggcttgtcg aagatcgtca ggacgtgcag ctcgccatcg gtcagcaacc
       61 cttcgacggc gacctcttct cccggcatgt agtcctcgat gaggatcccg gccgtgttca
      121 ggtcggtgcc gtcgctggtc aggatggctc ggagacggtt gacggctgcg atgagttcgt
      181 ccgcgtcgtt ggcgcggatg acgccgcgac ttccagacaa ccgcgttggc ttcacgaccg
      241 atgggtaggt gacctcattc gcgtaggtgc gcggatcggc gccggccggg aaggttcgga
      301 agccgggaca gcggacgccg ccggcagcca gcatcgagcg catcttgaat ttgtcgcggg
      361 cggcgacggc ggcgtccggc gcgttgtgcg gcacgccgag caggctcccg gccagcgccg
      421 ccagttcggt ggcgctgtcg tcgaccgaca gcagcgcatc gacgcggtcg gacttggcca
      481 gttcaacgat tgttgttgtt gcccggccgg cgtcgctgaa gtcgacgccg tgcgactggc
      541 cccagtactc ctggaggtgg tccggcatat cgacgaccga caccgtctcg atgccgagtc
      601 ccttggcggc ctcatagaag gcggccgccc ggtaggtggc cggggacatc agcaacatga
      661 cccgcttgat tgcattctgc ttcgtgcctg gcgtcgtcat ccctcaacca cctgtgtgtc
      721 tgcgttcgaa tcctgctctc agtctatcca agtacgcggc gcgtgtctgt attcccggtc
      781 gcgatcggcg attgacggga ttgatatgat tggagatgca caattgagac aggcgttgac
      841 ggaaacgagt aggcggatca acgctcgcca gcgagttcgg ggtggtggga gccggacggg
      901 agtcgttgcc gccgaagatc attccagagc cgccacccga acgggcgaaa ctggcgaagt
      961 tggtcgccca agtagacgtg gccgggcttg ccacccgtta tcacagtggc actgacgaac
     1021 gagcaacgtc gtcagagtga gcgggtcgac gacatcgttc gccggccaag cggggtggta
     1081 ccgcgggcgt tgagatttca gcccgtcctc gatgattcga gggcgggttt ttgtattgct
     1141 gaatacatgt ttgcagccgg cgacggaaca ggtgaagggg acaacgatgg cgaaagcact
     1201 gaaggcgttt cgagaggtcg acacgcgggc cgattttccg gcgatggagc gtgagcggct
     1261 caggcactgg tacgaagacg ggatcgtcga gcgctacctg acgaagaacg acgactccga
     1321 gacgcgctgg tcgttcctgg atgggccgat cacggccaac aacccgatgg gcgtccatca
     1381 cgcctgggga cggacgtaca aggatctgta catccgttac cacacgatgc ttgggcacaa
     1441 gcagcgctac cagaacgggt tcgactgcca gggactctgg gtcgaggtcg aggtcgagaa
     1501 ggaacttggg ctggcgacca agcgcgacat cgaggagttc gggatcgacc gcttcgtcga
     1561 gctgtgcaag gagcgcgtgc tcaagtacgc ggggatccag accgagcaga ccaaacggct
     1621 cggcaacttc atggactggg acaactcgta ctacacgatg tccgacgtca acaactacac
     1681 gatctgggat tttctgaaga cctgcaacga gaagggctgg atctacaagg gcgacgacgt
     1741 gatgacctgg tgcccgcgct gcgggaccgg catctcggag atggaactca acgagggtta
     1801 tcaggaccgg acgcaccgct cggtctatgc ctacttcccg ctggaagacg aggacgcctc
     1861 gctactggtc tggacgacga cgccgtggac cttgacgagc aacgttgcgg cggcggttca
     1921 tccggagctg acctacctga aggtccgaca aggcgagcat gtcttctatc tggcgaagga
     1981 cgctgcccag cacgcgttga agggcgagtt cgatacgctg gcggaggtga cgggcgcaga
     2041 cctggtcggg cgacgctatc gggggccgtt tgacgaactg ccggccaacg ccgatgtcga
     2101 gcatcgggtg attgcctggg acgatgtcgc ggctgacgag gggaccggga tcgtccatat
     2161 cgcgcctggt tgcggtaagg aagactacaa gctctcgaag gagcaggacc tgcaggtcgt
     2221 cgcgccgatc gacgagctgg gcaactacgt tgccggcttc gactggctga gcggcatgta
     2281 cgtccatgag gtcgcgatgc cgatcgtgcg caacctgcgc gagaaagggc tgctctaccg
     2341 cgatcacgag tacacgcacc gctacccgac ctgctggcgc tgcaagaccg acctggtctt
     2401 ccggctggtc gacgagtggt acatcggcgt cgatggcatg cgcgagcgga tgatggagct
     2461 gacgcgcgag attcgctgga tccccgagtt cggcgaggag cgcgagatcg actggctgcg
     2521 caacatggac gactggatga tctccaagaa gcgctactgg ggactggcgt tgccgatcta
     2581 cgagtgcccg gactgcggca cgtttgaggt gatcgggagc aaggaggagc tgggcgagcg
     2641 ggctgtcgag ggctgggagg agttcgaggg acacacgccg caccggccgt ggatcgacag
     2701 cgtcaagatt cagtgcaagg agtgcggcca gatcgtctcg cggatcaagg atgtcggcaa
     2761 cccgtggctg gacgccggga tcgtgccgtt ctcgacgatg ggctacaccg aggaccgcga
     2821 ggagtggcgc aagtggtttc cggccgactt catcaccgag tcgtttcctg gccagttccg
     2881 caactggttc tattcgatgc tggcgcagtc gacggcgttg acgggcgagg cgccataccg
     2941 gacggtgctg ggcttcgcgc tgctgcgaga cgagcacggc aaggagatgc acaagagcgc
     3001 gggcaacgcg atctggttcg atgatgccgc tgacgtgatg gggtccgacg tgatgcgctg
     3061 gctctactgc cagcacaacc ccgcccagaa cctgaacttc ggctatcaca tcggcgacga
     3121 gatgcggcga cgcttcatca tcccgctctg gaactcgtat gcgttcttca gcaactacgc
     3181 ggcgctggac gggttcgatc cgaccgaccc gtccaacgac gtgccgctgg cggaacggac
     3241 gatgctcgac cgctggatca tctcgcgctt gcaagggctg atcggtgatg tccgggcggc
     3301 gctggacgac tacgatcagg taacggcgac gcgagcgttc gagcgcttcg tcgtcgagga
     3361 gctgtccaac tggtacatcc ggcgtaaccg gcggcgcttc tggaagacgg aggcggaccg
     3421 ggacaaggcg gcagcctacc tgacgctcta cgaggtgctg gtgacgctgt ccgggttgct
     3481 ggcgccgttc gtgccgttcc tggcggacga gatgtacgac aacctggtcc tgtcggtcga
     3541 gccggatgcg gcgccgtcgg tccacctgac ggactatccg gtcgcggacg gcgcgaaggt
     3601 cgatgctgca ctggatcgcg acatggcggc gttgctgcag gccgtcaacc tcggccgggc
     3661 ggcgcgcaac aaggcgaatg tcaaggtgcg gcagccgctg ccggaggtac gcgtctgggc
     3721 gcgcgacgag gcgacgctgg cggcggtcga gcggatgcag gaccagctgc tcgacgagct
     3781 gaacgtcaag ggactcggac ggatcgacga tccggcgcag tacgcgacct acgtgatcag
     3841 gccgaacctg tcgctgctgg gtccgaagta cggcaagcgg ctcggcgagc ttcgcgggct
     3901 gctggcgggg gccgacccag caacagtggc ggcgacgatc cgctccggcg acgagctggc
     3961 tctcggcgag ttccggctca cccctgacga ggtgctggtc gacgtgaaag agcg
//
LOCUS       DASUGJ010000264         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_594384, whole genome shotgun sequence.
ACCESSION   DASUGJ010000264 DASUGJ010000000
VERSION     DASUGJ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594384"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1967
                     /locus_tag="VFV93_05845"
     CDS             225..1967
                     /locus_tag="VFV93_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5164899.1"
                     /translation="MASEWNARRGDWKRAGLARNRRGAALSRRSLLAGTAAGAAAGAF
                     GRFAPGGIGTPTARAQDVQPGGTLTYGLSFDFDGTLDPQVTNYDSSIRVMLNVCEPLL
                     WMATATDFYPALAESWEVSDDGLTYTFHLKKDVKFHDGTPFNADAVKFTYDRVVEGRN
                     LTAQGKEVDPKKVIVPGQSFNQIDAYDHAEIVDDYTIKLILSRPFGGFLTGLNGYLGI
                     VSPTAVEKMGLADFGRKPVGTGPFMIQEWVDADHVTLVKNPDYNWGSSFFKHSGAAYL
                     DQIIYKIIPDNAVRTGTLLSGESQYVDNVDALQLADLQENPDLAIIQQGQPGSGDILL
                     FNLARTDSPQADIAVRRALSYAIDKDAFNQAAFGGIYSPAASPLMKPTFGYEPKTEEL
                     YGYDPAKAASMLDEAGWTLNGEIREKAGKKLAFYWPIQDRPDDKAMATFIQGAFRAVG
                     ADVTVEPMEHGAFEEKTRTAGDYDLAFMWFSYADPDTLRTIFYSGNIGNFNVGKYNDP
                     EMDKMLLDAAASNDPEERQALYSKIQLKVLDEAVTIPLDDTITYNGKQASLEGDFLDF
                     LASYVWMNDAHFTQ"
BASE COUNT          404 a          731 c          663 g          353 t
ORIGIN      
        1 tcccgcagcg gtcaatcata gctctctgca atgcgacacc gcgggcattg acacgctcgg
       61 caggcggcga taccctgccc cacgccttgc caatgagggg ccccggctcg actgacgatc
      121 gccgtcacgg cgacgctggc cgcgtgggat gagccgcgga gactcttcgc tcggcgcttc
      181 gactgtcgcg cgcgtcagcg tcgccgttta caaggaggag cacaatggca tcggaatgga
      241 atgcccgccg gggagactgg aagcgcgcag ggttggcgcg gaaccgccgt ggcgcggccc
      301 taagccgtcg ctccctgctc gccgggacgg ccgcgggcgc cgccgcgggt gcattcggac
      361 ggttcgctcc cggcggcatc ggcacgccca cagcgcgagc gcaggacgtg cagccgggcg
      421 ggacgctgac ctacggcctg agcttcgatt tcgacggcac actcgatccg caggtcacca
      481 actacgactc ctcgatccgg gtcatgctca acgtctgcga gccgttgctc tggatggcga
      541 cggcgaccga tttctatccc gcgttggcgg aatcctggga ggtctcggac gacggactga
      601 cctacacttt ccatctcaag aaggacgtca aattccacga cggcacgccg ttcaacgccg
      661 acgccgtcaa gttcacctac gatcgcgtcg tcgaggggcg caacctcacc gcgcagggca
      721 aggaggtcga cccgaagaag gtcatcgtcc ctgggcagtc gttcaaccag atcgacgcct
      781 acgaccacgc cgagatcgtc gatgactaca ccatcaagct catcctgagc cgccccttcg
      841 gcggcttcct gaccgggctc aacggctatc tcggcatcgt ctcaccgacc gcggtggaga
      901 agatggggct cgccgatttc gggcgcaagc cggtcggcac cgggcccttc atgatccagg
      961 agtgggtcga cgccgatcac gtcacgctcg tgaagaaccc ggattacaac tggggttcct
     1021 ccttcttcaa gcactccggt gctgcctacc tcgaccagat catctacaag atcatccccg
     1081 acaacgccgt gcgcaccggc acgctcctct ccggcgaatc gcaatacgtc gacaacgtcg
     1141 acgcgctgca gctcgccgat ctgcaagaaa atccggatct cgccatcatt cagcaggggc
     1201 agcccggctc gggcgacatt ctcctcttca atctggcgcg caccgacagc ccgcaggctg
     1261 atatcgccgt gcgccgtgcc ttgtcgtacg cgatcgacaa ggacgcgttc aaccaggcgg
     1321 cattcggcgg catctactcc cccgccgcga gcccgctgat gaagccgacc ttcggctacg
     1381 agccgaagac ggaagagctg tacggctacg atccggcgaa agcggcgtcc atgctcgacg
     1441 aggctggctg gacgctcaac ggcgagatcc gggagaaggc tggcaagaag ctcgccttct
     1501 attggccgat ccaggaccga cccgacgaca aggcgatggc gacgttcatt cagggcgcct
     1561 tccgcgccgt cggcgccgat gtgacggtcg agccgatgga gcatggcgcc ttcgaggaga
     1621 agacgcggac cgcgggagac tacgacctcg ccttcatgtg gttctcctac gccgacccgg
     1681 acaccctgcg caccatcttc tactcgggca acatcggcaa cttcaacgtt ggcaagtaca
     1741 acgatcccga gatggacaag atgctcctcg acgccgcggc gagcaacgac ccggaggaac
     1801 ggcaggcgct ctactccaag atccagctca aggtgctgga cgaggcggtg acaatcccgc
     1861 tggatgatac gatcacctac aacggcaagc aggccagcct ggagggcgat ttcctcgact
     1921 tcctggcatc gtacgtctgg atgaacgacg cgcatttcac gcagtgaggc gcggctgacg
     1981 cacggtcatc gcctgccagg tgcgttgtcc aacgcgcctg atcccggcct cgcggcaact
     2041 gcggccgcga ggacggtggc ggctgatggc cgagagccgt taggcttcgc cctgccatga
     2101 cgcgctacat cctgcgccgg ttcgtgcaag cgatcccggt cctgttcggc a
//
LOCUS       DASUGJ010000265         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1290236, whole genome shotgun sequence.
ACCESSION   DASUGJ010000265 DASUGJ010000000
VERSION     DASUGJ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290236"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VFV93_05850"
     CDS             complement(<1..1289)
                     /locus_tag="VFV93_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164900.1"
                     /translation="MSRDAMLERNEPWDEDDTPRLSMLDRGIEIPLVAGRWLAATAAI
                     LIAILLRFLGLDRFALSAHEADIALAARNLVHGDSVPSDLYGMPFAIDWTALFFFGGG
                     SADSVGRIAMATAGVLVVIGLLWCGHWIGGRTATAAAFLAAFSPTLVAASRRLDGGIL
                     LVALSVLIVASVLFGRGRDSLAWPAIAGAATGLLVVSHPLGIPAAMLAWFGNYLLDRP
                     RHIARREAMLAGLAAGIGAAALATTALLSRPGSFSASIGEVLSLLWDDHLSEIGSRWY
                     MPTFNLILNEPILIALALIAGFASHERIVVRSIATWFFTALIIVSLLGNVGMPGYAIV
                     TLPLVLLAGIGVSYLIERLPWHAVRTGPATLYAGAVLLMAIAVVSLLGLITSGVSDDT
                     GDWLLRFALIVIVAILPLSFAITSIGSRLPGDRLMLVL"
     gene            complement(1315..>2685)
                     /locus_tag="VFV93_05855"
     CDS             complement(1315..>2685)
                     /locus_tag="VFV93_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HEX5164901.1"
                     /translation="RDRRSVWIGIALGLMLFAMFFTSMYTNMFGLGSGTVGALGYWLG
                     QHDVQRADQPWFYYLLMLPQYELVAVVLFPIGAFVTLRRLIPAIRRGEPVGRRNYMRG
                     LFIYWAIVNLAIFSWAGEKMPWLTVHMALPMILLAASMIGGGIEAIEAAIRREQLPRR
                     MIWLAGAGIPLISAGWFALWSWGSAGAWEERDGFLQRSLRPEVADYPWLLYLPLLALI
                     GLVAFVIWKLGSRLALPLLGLAMVAVILAGQIHTSLRLTYFDGDVPVDMLIYVQTSPD
                     VTRVVEEIGLMSREQTGGLDIPIAYDSGTSWPFQWYLRDYTHRRFFGSTLNEPPDEPI
                     VLISNEHVFDGDNEEMLAGYTYTEYVMRWWFPEDETYRRFAIAPELNKTDRQNYQTDE
                     PGPYSALDVAESVWRSLWGLREPQQQAKMFRLASYRELWAPIGSYNFRVYVRNDLLQT
                     WDDLRY"
BASE COUNT          534 a          855 c          925 g          371 t
ORIGIN      
        1 agcaccagca tcagccggtc gccgggcagc cgcgagccga ttgacgttat tgcaaacgag
       61 agcggcagga tcgcgacgat cacaatcagc gcgaagcgca gcagccagtc gccggtatcg
      121 tcagagacgc cggaggtgat caggcccaac aggctgacga cagcgattgc catcagcagg
      181 acggcgccgg catagagtgt ggccgggccg gttcgaaccg cgtgccacgg caggcgctcg
      241 atcaggtacg agacgccgat gccggccagc aggacgagcg gcagcgtcac gattgcgtag
      301 ccgggcatgc cgacgttgcc gagcaacgag acgatgatca gggccgtgaa gaaccaggtc
      361 gcgatcgaac ggacgacgat ccgctcgtgc gacgcgaacc cggcaatcag ggcaagggcg
      421 atcaggatcg gctcgttgag gatcaggttg aaggttggca tataccagcg tgagccaatc
      481 tcgctgagat gatcgtccca cagcaagctg agaacttcgc caatcgaggc cgaaaaggaa
      541 ccggggcggg agagcagcgc cgtcgtcgcc agcgcggcgg cgccgattcc tgctgccagt
      601 ccggccagca tcgcctcgcg gcgggcgatg tgacgtggcc ggtcgagcag gtagttgccg
      661 aaccaggcca gcatcgcggc cgggatgccg agcgggtgac tgacgaccag cagcccggtc
      721 gcggccccgg cgatagccgg ccaggcgagt gagtcccgcc cccggccgaa gaggaccgag
      781 gcgacgatca ggactgaaag cgcaaccagc aggatgccgc cgtcgagccg ccgtgatgcg
      841 gccaccagcg ttggcgagaa cgcggccagg aacgcggcgg cggtcgcggt ccggccaccg
      901 atccaatggc cgcaccagag cagcccgatc acgaccagga cgccggccgt cgccatcgcg
      961 atccggccaa cactgtcggc cgagccgccg ccgaagaaga agagggcggt ccagtcgatc
     1021 gcaaacggca tcccgtacag gtcggacggc accgaatcgc cgtggacgag attgcgggct
     1081 gccagcgcga tgtcagcctc gtgggcgctg agtgcgaagc gatccagccc gaggaagcgc
     1141 agcaaaatcg cgatcaggat ggcagccgtc gcggccagcc agcggccggc taccagcggg
     1201 atctcgatgc cccggtcgag catgctcaga cggggcgtgt cgtcctcgtc ccatggctca
     1261 ttgcgctcga gcattgcgtc acggctcaag tgatcggtgt cctttcggtc gttctcagta
     1321 gcggagatca tcccaggttt gcagcaggtc gttgcggacg tagacacgga agttgtacga
     1381 gccaatcgga gcccagagct cgcggtagga cgccagccgg aacatcttgg cctgttgttg
     1441 cggctcgcgc agcccccaga gcgagcgcca gacactctcg gcaacgtcga gcgcgctata
     1501 cggccccggc tcgtcggtct ggtagttctg acggtcggtc ttgttcagct cgggcgcgat
     1561 cgcgaagcgg cggtaggttt cgtcctccgg gaaccaccag cgcatgacgt attcggtgta
     1621 ggtgtagccg gccagcatct cctcgttgtc accgtcgaag acgtgctcgt tggagatcag
     1681 gacgatcggc tcgtctggcg gctcgttgag ggtcgacccg aagaagcgcc ggtgcgtgta
     1741 gtcgcgcagg taccactgga acggccagga ggtgccggag tcgtaggcga ttgggatgtc
     1801 gagcccgccc gtctgctcgc gggacatcag cccgatctcc tcgacgacac gggtgacgtc
     1861 cggcgaggtc tgaacataaa tcagcatgtc aaccggcacg tcgccgtcga agtaggtcag
     1921 ccgcagggag gtgtgaatct gcccggccag gatgacggcg accatcgcca gccccagcag
     1981 cggcaaggct aaccggctgc cgagcttcca gatgacgaag gcgaccagtc cgatcaaggc
     2041 cagcagcggc aggtagagca gccaggggta gtcagcgact tcggggcgga gcgagcgctg
     2101 caggaacccg tcgcgttctt cccaggcgcc ggcgctgccc cacgaccaga gcgcgaacca
     2161 gccggcggag atgagcggaa taccggcgcc cgccagccag atcatccgac gaggaagctg
     2221 ctctcgacgg attgcggcct cgatcgcctc gatgccaccg ccgatcatcg acgcggccag
     2281 caggatcatc ggcaaggcca tgtggaccgt cagccacggc atcttctcgc cggcccagga
     2341 gaagatcgcc agattgacga tcgcccagta gatgaacagc ccgcgcatgt agttgcgccg
     2401 gccgacgggc tcgccgcgcc ggatcgccgg aatcagccgc cggagcgtga cgaatgcgcc
     2461 gatcgggaag aggacgacgg ccaccagctc gtactgcggc agcatcagca ggtaatagaa
     2521 ccagggctga tccgcgcgct ggacatcgtg ctggccgagc cagtagccga gcgcgccaac
     2581 ggtgccgctg cccaggccga acatgttggt gtacatgctg gtgaagaaca tcgcgaacag
     2641 catcagcccc agcgcgatcc cgatccagac cgagcgccgg tcgcg
//
LOCUS       DASUGJ010000266         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1985199, whole genome shotgun sequence.
ACCESSION   DASUGJ010000266 DASUGJ010000000
VERSION     DASUGJ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985199"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /gene="aroA"
                     /locus_tag="VFV93_05860"
     CDS             complement(<1..1121)
                     /gene="aroA"
                     /locus_tag="VFV93_05860"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5164902.1"
                     /translation="MRLPDPLEVPVAPGPISGRVTLPGSKSYTNRALPIAALADGLST
                     ISGALDSDDTRYMVDALSQLGIQVEADWTTAEIRVSGQGGIVPAERAELYLGNSGTSM
                     RFLTALATLAHGDIRLDGTERMRERPLAPLIDGLAELGVNIRSEADNGCPPVNVRSAG
                     LPGGTLRMRGDISSQYFSAVSMILPYAQAPLMIEVLGELVSKPYIDMTAGTMAAFGVT
                     LQHDSYQRLWVEPGQHYHARRYVVEPDASAASYFFALAAVTGGTITVEHLPPSSRQGD
                     LAFVDVLEQMGCTVERRADDVTVTGAASLGGIEIDMNAISDTVPTLAAIAPFASEPVT
                     IRNVAHIRVKETDRIAAVVTELRRMNVDVDELDDGLVIRP"
     gene            complement(1118..1588)
                     /locus_tag="VFV93_05865"
     CDS             complement(1118..1588)
                     /locus_tag="VFV93_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX5164903.1"
                     /translation="MVSRQRIEGVEIKRLTTHADERGSLTELLRRDDEIFDGFGQCYV
                     SVSYPGVIRAWHYHKKQTDYFTCITGMIKVPLYDMREGSPTYGVVNEFFMGEDNRIVV
                     KIPIGVLHGFKNVGVTPCYLINFPTQPYDPADPDEYRIPYDSPDIPYNWDIVFT"
     gene            complement(1600..2394)
                     /locus_tag="VFV93_05870"
     CDS             complement(1600..2394)
                     /locus_tag="VFV93_05870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5164904.1"
                     /translation="MSDEHSPDDPYLRFAGYYDLEYATHTADLDFYREFAYQAGGPIL
                     ELGCGSGRVLTALVETGLELTGLDNSPAMLTLARESLDSDVRLVECSMDAVAECAELR
                     PDHYWMAYSAINTFLHLPDTTAQLRALEGLRRVVVQGGLLLLDLMTPDPHYLAGLDGR
                     VVHELSATLPDGNRLDKWAVRTHDFATQTIDTTVFFDTTDATGTVSRVSGRYLTRYIH
                     VFELEHLLERAGWRLISLYGSYDLSPFSSDSERMIALATWGSIETE"
     gene            complement(2433..>2634)
                     /locus_tag="VFV93_05875"
     CDS             complement(2433..>2634)
                     /locus_tag="VFV93_05875"
                     /inference="COORDINATES: protein motif:HMM:NF028211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5164905.1"
                     /translation="AVERERAVTIGDVLLRRTMLGLRPDLGMSLLEPALAVARHHLSW
                     SEARLAGERRRYLAEIDRLRVH"
BASE COUNT          467 a          848 c          848 g          471 t
ORIGIN      
        1 ggccggatga cgagtccgtc gtccagctcg tcgacatcga cgttcatgcg gcgcagttcg
       61 gtcacgacgg ccgcgatccg gtcagtctcc ttgacgcgaa tgtgggcgac attgcggatg
      121 gtgaccggtt cgctcgcgaa cggtgcgata gccgccagcg tcggcaccgt gtctgagatc
      181 gcgttcatgt cgatctcgat tccgccgagc gacgctgcgc cggtgacggt cacgtcgtct
      241 gcacgccgtt cgaccgtgca gcccatctgc tccagtacgt cgacaaacgc cagatcgccc
      301 tgtcgcgatg acggcggcaa atgttcgacg gtgatcgtcc cgccggtgac ggccgccagg
      361 gcgaagaagt agctcgcagc cgaggcgtca ggttccacga cataacggcg agcatgatag
      421 tgctgccccg gctcaaccca gagacgttgg taactgtcgt gctgcagcgt gacaccgaac
      481 gcggccatcg tcccggccgt catgtcgatg tacggcttcg agacgagttc gccgagcacc
      541 tcgatcatca acggtgcctg cgcgtagggc aggatcatcg atacggcact gaaatactgg
      601 ctggagatgt cgccacgcat ccgaagcgtt ccgcccggca ggccggccga ccggacgttg
      661 accggcgggc agccgttgtc cgcttccgag cggatgttga cgcccaactc ggccaggccg
      721 tcgatcagcg gtgcaagcgg ccgctcacgc atgcgctcgg tgccgtccag ccggatgtcg
      781 ccgtgagcca gggtcgccag cgccgtcagg aagcgcatgg aggtgccgga gttccccaga
      841 tacagttcgg cccgttcggc cgggacgatc ccgccctgac cgctgacgcg aatctccgcc
      901 gtagtccagt ctgcctcgac ctgaatgccg agttggctga gcgcatcgac catgtagcgc
      961 gtgtcatcgc tgtcgagcgc gccgctgatc gtcgagagac cgtcggccag cgccgcgatc
     1021 gggagcgccc ggttggtgta gctcttcgag ccgggcagcg tcacccggcc cgagattggc
     1081 ccaggcgcga cgggaacttc cagcgggtcc ggcaatctca tgtgaagacg atgtcccagt
     1141 tgtaggggat atccggcgag tcgtacggga tgcggtattc atcgggatcg gcagggtcgt
     1201 atggttgggt cgggaagttg atcaggtagc agggggtgac gccgacgttc ttgaagccgt
     1261 gcagcacccc gatggggatc ttgacgacga tccgattgtc ctcgcccatg aaaaactcgt
     1321 tgacgacgcc gtaggtcggc gagccctcgc gcatgtcgta gagaggaacc ttgatcatcc
     1381 ccgtgatgca ggtgaagtag tcggtctgct tcttgtgata gtgccaggcg cgaatgacgc
     1441 cggggtagga gacggacacg tagcattgcc cgaacccgtc gaagatctcg tcgtcgcggc
     1501 gcagcagctc ggtcagcgat ccgcgctcgt cggcatgggt cgtcaggcgc ttgatttcaa
     1561 cgccctcgat tcgctgtcgt gagaccacag ttcatccctt cactcagttt cgatgctacc
     1621 ccaggtcgcc agcgcgatca tccgctcact gtccgacgag aatggtgaca aatcgtatga
     1681 cccatacaac gagatcagcc gccagccggc ccgttccagc aggtgctcaa gctcgaagac
     1741 gtgaatatac cgcgtcagat accggccgct gacgcggctg accgtgccgg tggcgtcagt
     1801 cgtgtcgaag aagacggtcg tgtcgatcgt ctgcgtcgcg aagtcgtgcg ttcggaccgc
     1861 ccacttgtcg agccggttac cgtccgggag cgtcgccgac agctcgtgga cgacgcgacc
     1921 atccagcccg gccaggtagt gtgggtccgg cgtcatcaga tcgagcagca gaagcccgcc
     1981 ctgaacgaca acccggcgca gtccctcaag cgcccgcaac tgtgctgtcg tgtctggcag
     2041 gtgcaggaac gtgttgatgg ccgagtacgc catccagtag tggtccggtc gaagctcggc
     2101 gcactctgca accgcgtcca tgctgcactc aaccagccgc acgtcgctgt ccagcgattc
     2161 gcgcgccagc gtcagcatcg ccggcgagtt gtcaagtccc gtcaactcga gacccgtctc
     2221 taccaacgcc gtcagcaccc ggcccgatcc gcagccgagt tccaggatcg gcccgcccgc
     2281 ctgataagcg aactcacggt agaagtcgag gtcggctgta tgtgtggcgt attccaggtc
     2341 gtagtagccc gcgaagcgca aatatggatc atcgggcgaa tgctcgtcac tcatgccgcg
     2401 gattgtacgc tgccggcccg gtaggcatgc ggtcagtgca cccgcaaccg atcgatctcg
     2461 gccaggtagc gacggcgctc gccggccagc cgcgcctcgc tccaactgag gtgatgacgt
     2521 gcgacggcca gggccggctc cagcaggctc atgcccagat ccggccgcag tcccagcatc
     2581 gttcgccgta gcaggacatc gccgatcgtg acggcgcgct ctcgctcgac tgcg
//
LOCUS       DASUGJ010000267        11289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_594865, whole genome shotgun sequence.
ACCESSION   DASUGJ010000267 DASUGJ010000000
VERSION     DASUGJ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11289
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594865"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VFV93_05880"
     CDS             complement(<1..161)
                     /locus_tag="VFV93_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5164906.1"
                     /translation="MNPTATTDAGSGTLYLVAVPIGNIGDITYRAVETLRSVDVIAAE
                     DTRDFREIQR"
     gene            263..625
                     /locus_tag="VFV93_05885"
     CDS             263..625
                     /locus_tag="VFV93_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX5164907.1"
                     /translation="MRDDDQQAARVIHCPNCGASNPAGAGYCASCGSRLPDDTTADEE
                     TLIDVSSGQPRVLDEEPAGAFGSGRVQGGFTTVRFGQGRVVVSEGNRRTCLLVAVVAL
                     LVFCCACWVIWSIPGRLF"
     gene            complement(700..945)
                     /locus_tag="VFV93_05890"
     CDS             complement(700..945)
                     /locus_tag="VFV93_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164908.1"
                     /translation="MNGLTILLAIYALLYLPLTGYLYGRQRRWLGMLGWDLFVLGLLL
                     AFGGAGDAFAWAGLLWTAVAAAGLLLIVSDVVGMRRG"
     gene            1105..1458
                     /locus_tag="VFV93_05895"
     CDS             1105..1458
                     /locus_tag="VFV93_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164909.1"
                     /translation="MDAIMWLIIGLVSAWLAYSVTASERRSRSWGPVTAVGVYLALFG
                     AATGGLNWVYGLMRPKLVDDFPSENSLLFIGLVVLAGLVVLALDLLGTIDIMSQIQKS
                     INSGRPNPGGPSGDD"
     gene            complement(1502..1864)
                     /locus_tag="VFV93_05900"
     CDS             complement(1502..1864)
                     /locus_tag="VFV93_05900"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEX5164910.1"
                     /translation="MSIAYHLVPADWYRAQPPDRGYLPEPFETEGFVHLTHGLEDVLA
                     VGNLFYRADLRPYLLLTIDLERITAEVRYDDDSGRYPHVYGPLDRPAIVAIQRVEREP
                     DGTFTGFAPADDATPTRR"
     gene            2526..2693
                     /locus_tag="VFV93_05905"
     CDS             2526..2693
                     /locus_tag="VFV93_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164911.1"
                     /translation="MKKMMLEMRDRMKSRVGIYGTGWQSSSVMGLLLPLISVLLLSLV
                     GYMFIQTLHSI"
     gene            2806..3288
                     /gene="moaC"
                     /locus_tag="VFV93_05910"
     CDS             2806..3288
                     /gene="moaC"
                     /locus_tag="VFV93_05910"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX5164912.1"
                     /translation="MSRLTHFDEAGQARMVDVGDKAETHRVAIAGGRVRMQPETLRVI
                     VEGRASKGDVLGVAQVAAIMGAKRTHELVPMCHPLALTAINVALTPDVATSSVLIEAQ
                     VETRGKTGVEMEALTAVSVAALTIYDMVKAIDRGMQIEDIRLLHKRGGKSGDWQRGED
                     "
     gene            3498..3860
                     /locus_tag="VFV93_05915"
     CDS             3498..3860
                     /locus_tag="VFV93_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164913.1"
                     /translation="MLETVTLDPLARQLTATSADDVARVARTMRERRMLAEQAGRDAL
                     ATWYDAMLISVSSCGLMFRETRQIALAALPSRDLAALIVDFGREMAQIGPASGQRFEI
                     AATMLAELRNEMRRRRLA"
     gene            3970..4374
                     /locus_tag="VFV93_05920"
     CDS             3970..4374
                     /locus_tag="VFV93_05920"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX5164914.1"
                     /translation="MVETQVSKLTILLRGLTKRCPRCGEHDLFRRWTEMVDACPRCGL
                     HFEQEDGYWVGALTVNTIVSMVLFGLVISLTVALTWPDIPVYETMLFGSIAGIAFPYF
                     YYPFSKTNWVAIDLAFFHPELMQPGTGLHPRR"
     gene            complement(4477..5760)
                     /locus_tag="VFV93_05925"
     CDS             complement(4477..5760)
                     /locus_tag="VFV93_05925"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX5164915.1"
                     /translation="MRSVLLRSAAVLAALVLLLTSFSTTLAQSDNPAFRATWTRTDQP
                     VADDAVDRTWIWGPLTTAWGETEKYAEGVNGQRNVLYFDKARMEITHPDGNTDDLWYV
                     TNGLLVMEMISGQMQMGDDAFVDHGPADVPVAGDPDDTNGPTYATFTDLLDVSVDDPV
                     DVITRTVDRDGTVGDDASYATYAIGAHEYIAATDHWIADPFWTFMTSSGLVWEDGKLV
                     TDKLFEDPVYGTGYPVTEAYWATVAVGGTEMDVLMQCFERRCLTYTPGNDEGWQVEAG
                     NVGQHYYHWRYGHAMPGAETATAFMVALNDNGEIGIPVGCEDSLVEVTIPIWETGSLD
                     EKIAATLTVLLSYHDVDFGESGLRNALYQNDATVESVTVEDGVATVNLSGTIPSGGEC
                     DDPRIAGQLEETVKAFDGVDEAVILLNGEPLFPAP"
     gene            complement(5950..7038)
                     /locus_tag="VFV93_05930"
     CDS             complement(5950..7038)
                     /locus_tag="VFV93_05930"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX5164916.1"
                     /translation="MSATEILTRRLFGQRLAGTPFEQPEDVVRWLGCVQSQDPIGARW
                     SIGQRVAGCTDADVAQALDEGRILRTHILRPTWHYVMPEDIRWILQVTAPRVHALNAS
                     MYRQTELDDELLARSHALFTAALAGGGQLTRAELGSVLAEGGIVAEKLRLGLIVMHAE
                     LEGLICSGAVRGKQHTYALLVERAPAAVLLDPDEALARLARRYVSGHGPATDRDFAWW
                     ASLSLSQARRGLEMVKSNLRLLEHDGQQFWFSVDAQPVGSLPPVAWLLPEYDEAIWFR
                     SLAFPDVERTRDTSSWGDRFARPIIIGTRRAGLWRRTIARTSIALDALLFAPLTAAEQ
                     TAFDAAVDRYSTYIGLPVTVTYASNAIR"
     gene            complement(7052..7915)
                     /locus_tag="VFV93_05935"
     CDS             complement(7052..7915)
                     /locus_tag="VFV93_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5164917.1"
                     /translation="MPAIDRAGATLSWEEFGSGPAALFVHGGLFDPMTGERFWATPGV
                     AGDLAAACRVLVPDRRCSPGRTTAGFSVHSWEREAEDLAAILDAAGIERAHLVAGSNG
                     CSAIARLALSHPERVASLVCCWPAAPTTAALLASFERSAAAVERDGTAGYLDTLRREG
                     VPRPGEERAGFPFGFALLHDRQMAASFLECSGTDAARIFRETGAALLPGEPIRGLSGE
                     EAARIGTGGFPVYVMPAEPDDPYHPRAVALALRDVIPGAILTAGYPVTPSRFFADARA
                     GFAATLRGIVG"
     gene            8366..9403
                     /gene="pdhA"
                     /locus_tag="VFV93_05940"
     CDS             8366..9403
                     /gene="pdhA"
                     /locus_tag="VFV93_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX5164918.1"
                     /translation="MAKAEPAVAALQSMERDELLDMYRQMYLIRRFEQRSEEQYAYGK
                     IGGFLHVYIGEEAVAVGAIKALGPRDHLITHYRDHGYALAIGSDPGAVMAELFGKATG
                     TTGGRGGSMHLTHVERHFWGGYAIVGGHIPIATGLGLALQYQGIDGVAMCIFGDGSTN
                     AGAFHEALNMASVWKVPVIFLCENNLYAMGTAWEYVSAVQQMSTKAKAYDIPAEIVDG
                     QDVIAMYEATQRAVEHCKAGKGPYFLEAMTYRFRGHSMADPETYRDKEEIEKRRSDDP
                     LLLFSQKLLDAGIAQQSDLDAIHDEVEQVVEQAIEFADNSPVPDPNTIYDHIYANGSR
                     DAGTGTGGQGT"
     gene            9472..10521
                     /locus_tag="VFV93_05945"
     CDS             9472..10521
                     /locus_tag="VFV93_05945"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5164919.1"
                     /translation="MASTLEELQDMQRQAEEAAKRGKVEGDREVTYRDALREAIVEEM
                     ERNENVFLIGEDIGAYGGSYVVTRGFQETYGRKRVIDAPIAELGIVGTAVGAAMGGLR
                     PIVELMTINFSLLAIDQIVNHAAKIHHMFNGQFSAPVVIRTASGWGQLGATHSQTFEG
                     WFAHVPGLRVVMPSTPKDAKGFLKQALRGNDPVMFIEHSLIYRNRGIIPDGDYTLPLE
                     GAEVRREGTDITLVSWSRGMYLALGAAEELAEQGISAEVIDMRVLRPLDIETVVKSVE
                     KTNRLVIVEESWRTMGLGAEIAAAIQEHAFDYLDAPIGRVGSLEVPMPYAKNLERLII
                     PGKDEVVTAVKEALA"
     gene            10618..>11289
                     /locus_tag="VFV93_05950"
     CDS             10618..>11289
                     /locus_tag="VFV93_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5164920.1"
                     /translation="MAKTVVMPQMGYDMDAGTLLRWLKNVGDQVERGDAIAEIETDKV
                     NIEIEAFDAGVLRKTLITEGQSVPVGDPIAIIGTADEPIEGDTGAAEAAPQEQEAAQP
                     VAAAPASEAAAVNAESPVAAAQQGASQQGAVPPPTQQQPPAQPQAPSQVGTNGVQPQV
                     VDREPGERIRASPLVRRIAAEHEIDLALVNGTGPHGRIVKRDVEPFMTGAQPKPAAAP
                     QAQPQV"
BASE COUNT         1927 a         3691 c         3804 g         1867 t
ORIGIN      
        1 cgctggatct cacggaagtc gcgggtgtcc tcggccgcga tcacatcgac actccgcagc
       61 gtctcgaccg cccggtaggt gatgtcgccg atgttgccga tcgggacggc gaccaggtag
      121 agcgtcccgc ttccggcatc ggttgtcgct gtcgggttca gttgcgtccc ctgtccgtgt
      181 cacggctgga cagccggccc gtcctggatg ctattgttcc accaatcgcg ggtggaacga
      241 cgctgatcgg aagaggacga gcgtgcgaga tgacgaccag caagcggcac gggtgatcca
      301 ctgcccgaac tgcggcgcat cgaacccggc cggagccggc tactgcgcct cctgcggctc
      361 gcggctaccc gacgacacga ccgccgacga ggagacgctg atcgatgtct ccagcggcca
      421 gccgcgcgtc ctcgacgagg aaccggccgg agcatttggc agcggccgcg tccagggcgg
      481 cttcacgacc gtccgcttcg gacagggccg cgtcgtcgtc tccgaaggca accgccgcac
      541 ctgcctgctg gtcgccgttg tcgccctgct cgtcttctgc tgcgcctgct gggtcatctg
      601 gtcgataccg gggcggctgt tctgagcgtt acgggagtcg ggattcggga ttcggggttt
      661 gggacggcaa tgcgtcccgc gtcccgaatc ccgtaacgct caccccctcc gcatcccaac
      721 gacatcgctg acgatcagga gcaggccggc ggcggcgacg gcggtccaga gcaggccggc
      781 ccaggcgaag gcgtcgccgg cgccgccgaa ggcgagcagc aggccgagca cgaacagatc
      841 ccagccgagc atccccagcc agcgccgctg acggccgtag aggtagccgg tcagcggcag
      901 gtagagcagc gcgtagattg ccagcaggat cgttaacccg ttcacccaat ctcccactcg
      961 gtccggggca tcgccggcgg gcgtcccgcc ggcaaggcta cccgatttcg cgctaccggc
     1021 cgccgtccat cttcacaccg cgcgggttcc ctgctagagt ggacgcagtc accgtcaagc
     1081 gatatcagag tggaggcgac cgccgtggac gcaatcatgt ggttgattat cggcctcgtc
     1141 agtgcgtggc tggcctattc cgtaacggcg agcgagcgcc gaagccggag ttgggggcct
     1201 gtgacggctg ttggcgtcta cctggcgctc ttcggcgcgg cgacaggtgg cctcaactgg
     1261 gtctacggcc tgatgcggcc gaagctggtc gatgacttcc ccagcgagaa ctcgctgctg
     1321 ttcatcggcc tggtcgtgct ggcgggattg gtcgtgctgg cgctcgatct gctcggcacg
     1381 atcgacatca tgagccagat tcagaagagc atcaattcgg gccggccgaa cccgggcggc
     1441 ccctccggcg acgactaacc ctcgtcgtca cgcgcgaaga cgcgaggcgg tagaccctgc
     1501 gctaccgcct cgttggcgtg gcatcgtctg ccggtgcgaa cccggtgaag gtgccgtccg
     1561 gttctcgctc gacgcgctga atcgcgacga tcgccggccg gtcgagcggg ccgtagacgt
     1621 gcgggtaccg tccgctgtcg tcgtcgtagc ggacctcggc cgtgatccgc tccaggtcga
     1681 tcgtcagcag caggtatggc cggaggtcgg cccggtagaa cagattaccg accgccagca
     1741 catcctccag cccgtgtgtc agatggacga agccctccgt ctcgaatggt tccggcagat
     1801 agccgcgatc cggcggctgc gcgcggtacc agtcggccgg aaccaggtga tacgcaatcg
     1861 acatcagtgt ggtcctctcg tagagtctgc tgtctcggca gattcgctgg atgggcgacg
     1921 gtgtggcgag ggtgacccgc ccggcttccc gacctgaccg gtcaccctcg ccacaccgtc
     1981 gccgtacccc atcacgactg tgggcaggta gagccagaca cagcaacgcg gcgccggaat
     2041 accgacgccg cgttcaatgg gcaaaaggat aggccccgac gagccttgag tggttccgac
     2101 gctcaggtca tgaaattcct tggcccagat ccccggttgc ccggtcgtct gattcaggtg
     2161 atctcagaca cgtgctgggg acaagccccg cacaagattc caaacctggc cttcgtcgtt
     2221 tccgcgatgg tgattgaact gacgcccact cacgaccgcg atccggttcg acgcttcggt
     2281 tggatacctt cgtcgttcga tgaatcgatt gactccggtg cgtcggtcct tcgtaactcc
     2341 tcgggacctg tacatacact agcatgcgtt tgacaggctg tcaatacttt tttcgcagat
     2401 gcgtgtcgat tccctttcgg cgcgcacggt tgggcctctc ggtgctagaa tcgatgctcg
     2461 gatggtggcg cgcatcgccc ctcccgtagt ggctggtacg agcgtgtcga aggtggtggg
     2521 tagcaatgaa gaagatgatg ttggagatgc gcgatcggat gaaatcgcga gtcgggatct
     2581 acggcaccgg ttggcaatca tcttcggtca tgggcttgct actgcccttg atcagcgtcc
     2641 tcctgctgtc gctcgtcggc tacatgttca tccagacgct gcacagcatc tagcgttcgc
     2701 ccagcgcatc gcgacgatgc cgcctgccgg ctctggccgg ttgccggcgg ctcgtcattc
     2761 tcctcagccc gcagtgaccc gcccgcacca ctcgtaagga acccgatgag caggctcaca
     2821 cacttcgatg aggccggcca ggcgcggatg gttgacgtcg gcgacaaggc cgagacgcac
     2881 cgggtcgcga ttgccggcgg ccgggtgcgg atgcagccgg agacgttgcg ggtgatcgtc
     2941 gaagggcgcg ccagcaaggg cgatgtgctc ggcgtggccc aggtcgcggc gatcatgggc
     3001 gccaagcgca cccacgagct ggtgccgatg tgccacccgc tggcgctgac ggccatcaac
     3061 gtcgcgctga cgcccgacgt ggcgacctca tcggtcctga tcgaggctca ggtcgagacg
     3121 cgcgggaaga ccggcgttga gatggaggcg ctgacggccg tctcggtcgc ggctctgacc
     3181 atctacgaca tggtcaaggc aatcgatcgc ggcatgcaga tcgaggacat ccgcctgctt
     3241 cacaagcgcg gcggcaagag cggcgattgg cagcgcgggg aggactgagt ccctgctgcg
     3301 actgatacgc cggagcatcc ctcccggcgt tgcacgcgct tgtgacggct ttgtaacggc
     3361 cagatcgggt atttccgcgt atgctggccg tagcccgctg agtgattgtt gcgatgcgcg
     3421 gcaggcaagt tgcgagcaga ctgacacagt gggccatcaa tggagatggc aggatcgcgg
     3481 agaggcgacg acacccgatg cttgagacag ttaccctcga ccccctggca cgtcagttaa
     3541 cggcgacgag tgccgacgat gttgcgcggg tcgcgcggac aatgcgcgag cggcggatgc
     3601 tggccgagca ggccggccgt gacgcgctgg ccacctggta cgacgcgatg ttgattagcg
     3661 tcagctcctg cgggctgatg ttccgtgaga cgcgccagat cgcgctggcc gcgctgccct
     3721 cgcgggatct ggccgcgctg atcgtcgact tcgggcggga gatggcccag attgggccgg
     3781 ccagcggcca gcggttcgag attgccgcga cgatgctggc ggagctgcgc aacgagatgc
     3841 ggcgccggcg tctggcctga catctgattc ggtttcttgc gaatcggtgg agtatggttg
     3901 gatctggctt cggtaccgtc accggcgggc gtgtctgacc cccgggcggg tttctccagg
     3961 gcaggttcca tggtcgagac gcaagttagc aagttgacga ttctgctgcg tgggttgacg
     4021 aagcgttgcc cccgctgtgg agagcatgat ctcttcaggc gctggaccga gatggtggac
     4081 gcctgcccgc gctgcggtct gcacttcgag caggaagacg gctactgggt tggcgcgctg
     4141 acggtgaaca cgatcgtcag catggtcctg ttcgggctcg tcatcagcct gaccgtcgcc
     4201 ctcacctggc cggatatccc ggtctacgag acgatgctgt tcggctcgat cgccgggatc
     4261 gccttcccgt acttctacta cccgttctcg aagaccaact gggtcgcgat cgacctggcc
     4321 ttcttccacc ccgaactgat gcagcccggc accggcctgc acccccgccg ctaggcgcga
     4381 tccgccccgt gttgtcatcc ttcggctcac gcttcaggat gacaacacgg ggtgggcatc
     4441 cagccgatcg gatgcccgcc ttgtcgttcc cgagggctac ggcgccggga agagtggctc
     4501 gccgttgagc aggatgacgg cttcgtcgac gccgtcgaat gccttgacgg tttcctcaag
     4561 ctggcccgcg atgcgtgggt cgtcgcattc gccgccggag gggatcgtcc cgctcaagtt
     4621 gacggtcgcg acgccgtcct cgaccgtcac gctctcgacc gtcgcgtcgt tctggtacag
     4681 cgcgttgcgc aggccggact cgccgaagtc gacatcgtgg taagagagca gcaccgtcag
     4741 cgtcgcggcg atcttctcat cgaggctgcc ggtctcccag atcgggatcg tgacctcaac
     4801 caggctgtcc tcgcagccga ccgggatgcc gatttcgccg ttgtcgttga gggcgaccat
     4861 gaaggccgtt gccgtctcgg caccaggcat cgcgtggccg tagcgccagt ggtagtagtg
     4921 ctgaccgacg ttgccggcct cgacctgcca accctcgtcg ttgcccggtg tgtaggtcag
     4981 gcagcgtcgc tcgaagcact gcatcaggac atccatctcg gtgccgccga ctgcgaccgt
     5041 cgcccagtag gcctcggtga ccgggtagcc ggtgccgtag acgggatcct cgaagagctt
     5101 gtcggtgacc agcttgccat cctcccagac caggccggag gaggtcatga acgtccagaa
     5161 cggatcggca atccagtggt cggtggctgc gatgtactcg tgcgcgccga tcgcgtaggt
     5221 cgcgtagctc gcgtcgtctc cgaccgtacc gtcgcgatcg accgtccggg tgatgacatc
     5281 gaccgggtca tcgaccgaga cgtcgagcag atcggtgaaa gtcgcgtagg tcgggccgtt
     5341 ggtgtcgtcc ggatcgccgg cgaccggcac atcggccggg ccgtgatcga cgaacgcgtc
     5401 gtcacccatc tgcatctggc cgctgatcat ttccatcacc agcaggccgt tggtgacgta
     5461 ccagaggtcg tcggtgttgc cgtccgggtg ggtgatctcc atccgtgcct tgtcgaagta
     5521 caggacattg cgctggccgt tgactccctc ggcgtacttc tccgtctcgc cccaggcggt
     5581 cgtcagcggc ccccagatcc aggttcgatc gacggcgtcg tcggcgaccg gctggtctgt
     5641 ccgggtccag gttgcacgga aggccgggtt gtccgattgc gccaatgtgg tggagaagga
     5701 tgtcagcagg agcaccagcg cggccagcac tgccgcggaa cgcaaaagca ccgatcgcac
     5761 gaaacacctc gtcctttcgt tcggtcgtcg tgtccgattc tcaggggcat ctgaaccggc
     5821 cggttctctt tgtggaaaga cagaacctcc aaaagaggta cgtccctgcc ttcttccggt
     5881 acgggcgagg caccgactcg cccgtcgtgt tccgcccaaa cgcgtcaggt ggcgagccgg
     5941 gcgaggcccc taccggatgg cgtttgacgc gtaggtgaca gttaccggca gccctatgta
     6001 ggtgctgtag cggtcgacag ccgcgtcgaa ggcggtctgt tcggcggccg tgagcggggc
     6061 gaagagcagc gcatcgagcg cgatcgaggt gcgggcgatc gttctgcgcc agaggccggc
     6121 cctacgcgtg ccgatgatga tcggacgggc gaatcggtcg ccccagctgc tggtgtcgcg
     6181 ggtgcgctcc acgtcgggga aggccaggct gcggaaccag atcgcttcgt cgtattccgg
     6241 cagcagccag gcgaccggcg gcagcgagcc gaccggttgg gcgtcgacgc tgaaccagaa
     6301 ctgctggccg tcgtgctcca gcagccggag attagacttg accatctcca gtccccgccg
     6361 cgcctgcgac agcgacagcg acgcccacca ggcgaagtcg cgatcggtgg cggggccgtg
     6421 gccgctgacg tagcgccggg caagccgcgc cagcgcctcg tccggatcga gcagcactgc
     6481 cgccggcgcc cgttcgacca gcagtgcgta ggtgtgctgc ttgccccgga cggcgccgct
     6541 acagatcagc ccttccagct cggcgtgcat cacgatcaga cccagccgca gcttctcggc
     6601 gacgattccg ccctctgcca ggacggaccc gagttcggcc cgggtgagtt gcccgccgcc
     6661 cgcaagcgcg gccgtgaaca gcgcgtggct gcgggcgagg agctcgtcgt ccagctcggt
     6721 ctgccggtac atcgacgcgt tgagggcgtg gacgcgcggg gccgtgacct gtaggatcca
     6781 gcggatgtcc tccggcatga catagtgcca ggttggacgg agaatatgcg tgcgcaggat
     6841 gcggccctcg tcgagcgcct gcgcaacatc agcgtcggtg cagccggcga cccgctggcc
     6901 gatcgaccag cgcgctccga tcgggtcctg cgactggacg cagcccagcc agcgcacgac
     6961 atcttccggc tgctcgaacg gcgtcccggc cagtcgctgg ccaaacagtc gccgggtcag
     7021 gatctcggtc gcactcatgc ggcgcagtct atcatccgac gatgccacgc aaggtcgccg
     7081 cgaatcccgc ccgggcgtcg gcgaagaagc gtgacggcgt gaccgggtag ccggcggtca
     7141 gtatcgcgcc agggatcacg tcacgcaggg caagggcgac agcgcgggga tgatacgggt
     7201 cgtccggctc ggccggcatg acgtagacgg ggaacccacc tgtgccgatc cgggcggcct
     7261 cttcgccgga caggccacgt atcggctcgc cgggcagcag ggccgcgccg gtctcgcgga
     7321 agatgcgggc tgcatcagtg ccgctgcact ccaggaacga agccgccatc tgccggtcgt
     7381 gcagcagcgc gaagccgaag gggaagccgg cccgctcctc gccgggacgc ggcacgccct
     7441 cgcgccggag tgtgtcgagg tagccggctg tgccgtcccg ctcgacagcg gcggccgacc
     7501 gctcgaacga ggccagcagt gccgctgtcg ttggcgcggc cggccagcag cacaccagcg
     7561 aggccacccg ctccggatgc gacagcgcca gccgggcaat cgccgagcag ccgttcgacc
     7621 cggcgacgag gtgcgcccgc tcgatcccgg cggcgtcgag gatcgcagcc agatcctctg
     7681 cctcgcgctc ccagctatgg acgctgaagc cggccgtcgt ccggcccggt gagcagcgcc
     7741 ggtcgggaac cagcacccgg caggcagccg ccagatcccc agcgacaccc ggagttgccc
     7801 agaagcgctc gcccgtcatc ggatcgaaca gtccgccgtg gacgaacagg gcagccgggc
     7861 cgctgccgaa ttcctcccag gacagcgtgg caccggcgcg atcgatggct ggcatggagg
     7921 atcctttcgg ttggcgttcg tgacgcgtga cgcgggtgaa gttcgggccg ccgcgtcacg
     7981 cgtcacgcat cacgcatcac gaacgccgct tccgcggaat cgtgcggaaa accggccatc
     8041 cctacggcca accatagtac aattctgcga atacctgcgc ggtggcggtt tcagggaggc
     8101 gcctgccgcg cgcgtgagcc tgggctcggg caatcagagg gcgcgccacg gcggcccggc
     8161 tcattcagac aatgctcgtg tttgctgcga atgtcgacgc gcgcgccctg cgcgagcctc
     8221 ctggaaggct attccaagcg tgccaactgc tgatgacagt ccacgcgcac cctcaaggtc
     8281 gaggagccgc gcgaaagctg aacagaacac ggccacgaac acgacccgta ccggaacaac
     8341 ccgcaagaag ggacaaggac gcgcaatggc gaaggcagag cctgccgtag cggcgttgca
     8401 gtcgatggag cgcgacgagc tcctcgacat gtaccgccag atgtatctca tccgccgctt
     8461 cgaacagcgg tcggaggagc agtacgccta cggcaagatc ggcggcttcc tgcatgtata
     8521 catcggggag gaggcggttg cggtcggcgc gatcaaggcg ctcggcccca gggaccacct
     8581 catcacccac tatcgtgacc acggctacgc gctggcgatc ggctccgatc ccggcgcggt
     8641 gatggccgag ctgttcggca aggcgaccgg aacaaccggc ggacgcggcg gctcgatgca
     8701 cctgacgcat gtcgagcggc acttctgggg cggctacgcg atcgtcggcg gtcacattcc
     8761 gatcgcgacc ggcctcggcc tggcgctgca ataccagggg atcgacggcg ttgcgatgtg
     8821 catcttcggc gacggctcga ccaacgccgg cgcgttccac gaagccctca atatggcgtc
     8881 cgtctggaag gtgcccgtca tcttcctctg tgagaacaac ctctacgcga tgggtaccgc
     8941 ctgggagtac gtttcggctg tccagcagat gtcgaccaag gccaaggcct acgacattcc
     9001 ggccgagatc gtcgatggcc aggacgtgat cgccatgtac gaggcgaccc agcgggccgt
     9061 cgagcactgc aaggccggca aggggccgta cttccttgag gcgatgacct accgcttccg
     9121 gggccactcg atggccgacc cggagaccta ccgcgacaag gaagagatcg agaagcgccg
     9181 cagcgacgac ccactcctgc tgttcagcca gaagctcctg gacgccggaa tcgcccagca
     9241 atctgacctc gacgccatcc acgacgaggt cgagcaggtg gtcgagcaag cgatcgagtt
     9301 cgccgacaac agccctgtcc cagatccgaa cacgatctat gaccatatct atgcgaacgg
     9361 gagtcgggat gcgggaaccg ggactggggg acaaggaacg taagccagga cgagcgatca
     9421 atcctgaacc cgagtcccga gtcccgagtc ccgagtgaac ggagtgagca gttggctagc
     9481 acgttagaag aactacagga catgcagcgg caggccgagg aggccgccaa gcgcgggaag
     9541 gtcgagggtg accgcgaggt gacctaccgc gacgcgctgc gtgaggcgat cgtcgaggag
     9601 atggagcgca acgagaatgt cttcctcatc ggggaagaca tcggcgcgta tggcggctcc
     9661 tacgtcgtca cgcgtggctt ccaggagact tatggccgca agcgcgtgat cgacgcgccg
     9721 atcgccgagc ttggcatcgt cggcacggca gttggcgcgg cgatgggtgg cctgcgcccg
     9781 attgtcgaac tgatgacgat caacttctcc ctgctggcga tcgaccagat cgtcaaccac
     9841 gccgccaaga tccaccacat gttcaacggg cagttttcag cgccggtcgt catccgcact
     9901 gcctccggct gggggcagct tggcgcgacc cactcgcaga ccttcgaggg ctggtttgcc
     9961 cacgttcccg gcctgcgggt cgtcatgccg agcacgccga aggacgccaa gggcttcctc
    10021 aagcaggcgc tgcgcggcaa cgacccggtc atgttcatcg agcactcgct gatctaccgc
    10081 aaccgcggca tcatccccga tggcgactat acgctgccgc tcgaaggcgc cgaggtgcgc
    10141 cgcgagggga ccgatatcac gctcgtctcg tggtcccgtg ggatgtacct ggcgctcggc
    10201 gcggccgagg aactggccga gcagggtatc tcggctgagg tgatcgacat gcgcgtcctg
    10261 cgcccgctcg acatcgagac ggtcgtcaag tcagtcgaga agaccaaccg gctcgtgatc
    10321 gtcgaggaat cctggcggac gatgggcctc ggcgcggaga tcgccgccgc catccaggag
    10381 cacgccttcg actacctcga cgcgccaatc ggccgcgtcg gtagcctcga agtcccgatg
    10441 ccctacgcca agaacctcga acgactcatc atccccggca aggacgaggt cgtgacggcg
    10501 gtgaaggaag cgttggcgtg agccgtgacg cgtgacgcgt gacgcgtgac gcgctacggc
    10561 cggaggagaa tcccacgcgt cacgcatcac gcgtcacgcg tcacggaagg atgacagatg
    10621 gccaaaacag tcgtgatgcc ccagatgggc tacgacatgg acgcggggac gttgctgcgc
    10681 tggttgaaga acgtcggcga ccaggtcgag cggggtgacg caatcgctga gatcgagacc
    10741 gacaaggtca acatcgagat cgaggcgttc gatgccggtg tcctgcgcaa gacgctgatt
    10801 accgaggggc agtcggtccc ggtcggtgat ccgatcgcga tcatcggcac ggccgacgag
    10861 ccgatcgagg gcgatactgg cgctgccgaa gccgcgccgc aggagcagga agcggcccag
    10921 ccggtagccg ccgcgccggc cagtgaagcc gccgccgtca acgcagagtc gccggtggcc
    10981 gccgctcagc agggcgccag ccagcagggc gcggtcccgc cgcctaccca gcaacagccg
    11041 ccggcgcagc cgcaggcgcc gtcgcaggtt ggcacgaacg gcgtccagcc gcaggtcgtc
    11101 gaccgtgagc cgggcgagcg catccgcgcc tcgccgctcg tgcgccggat cgccgccgag
    11161 catgagattg acctcgcgct cgtcaacggc accggcccgc atggccggat cgtcaagcgc
    11221 gacgttgagc cattcatgac tggtgcccag ccgaagccag ccgccgcgcc acaagcgcag
    11281 ccgcaggtc
//
LOCUS       DASUGJ010000268         4939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1985307, whole genome shotgun sequence.
ACCESSION   DASUGJ010000268 DASUGJ010000000
VERSION     DASUGJ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985307"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFV93_05955"
     CDS             <1..996
                     /locus_tag="VFV93_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5164921.1"
                     /translation="LLRRRVTIDLRGQVVLITGGSRGLGLALAEAFVAEGAVPVICAR
                     NPDDLEDARQRLLRQGAADVLAVPCDVRVREQVERLIATTLEHFGRLDIVVNNAGTIM
                     VGPLESQTLADFEDGMATMFWGTVYPALAALPQMRRQGGGRIVNITSIGGKVSVPHLL
                     SYSAAKFAAVGFSEGLRAEVAKDGIDVLTVVPGLMRTGSHVNAIFKGRHEQEYTWFGL
                     GASMPGLTISAEHAAHEIVAAIRRGDAEVTLGLPAQLLAGFHGLFPGVTSDLMGLVNR
                     LLPSPGGDRDRHLGRDSETAVTESFLTTLGQRAAERYQPDEPRDRVLRETPRPVS"
     gene            1061..2563
                     /locus_tag="VFV93_05960"
     CDS             1061..2563
                     /locus_tag="VFV93_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164922.1"
                     /translation="MGRCRWTIAVATLLLVFPLLAACGDAESDGALADVAATPSEPVS
                     SPTETVATTASQTAPSASPTIVASASPDDEMTPVDFFELIDAALAGTDGILHTRVTYT
                     TFYQGQEAPIWTDESWIDAGRGVARREFQLAPDSSLDMSENMTYIVVGDTSYSINNDL
                     ATDGSYPDVDTTSVQTECATIETQVLVAFLVCGLYPFQPSEPQLSVEPNVEYEGQAAL
                     ALLYEMATAPSSDPAPRATPEPRPERVEARLFIARETSLPLAWVVDWNDAGGETLGSQ
                     VARFASEPVAAESLPADFFDPTSIGYSDDPMAPIDWADLDIPILWLGERFEPGGDLPL
                     LVLQYAEPQAKGQPDWASAHLQYYSADYSDDHLADTFVDMLLWTPEAWQRYSDELPGW
                     MPFKSICAERRELPVESGGTAVIILTHEMEESSVPQPQSTPVEPTPVADRPGCPDAPY
                     DRFAAIVTYDDVVVTVNVLDCLYCGPRPYKDDAYDTVPGLEAIVNGLRRY"
     gene            complement(2620..4245)
                     /locus_tag="VFV93_05965"
     CDS             complement(2620..4245)
                     /locus_tag="VFV93_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX5164923.1"
                     /translation="MTDLWYKNAIFYGVDIKTFCDSNGDGFGDLAGLTGKLDYFSDLG
                     VDCLWLLPFYGSPLADNGYDISDHCAINPRFGSFADFDHLIEEASRRGLRVLIDLVTN
                     HTSDQHPWFQRARRDPSSRYRDYYVWSNEPVELPGTKPIFPGAEDSVWEYDDMAGQYY
                     LHRFYCFEPDLDTFNPDVCAEILQIMEFWLRRGVDGFRIDAAPYLVRPKGARPAPNGD
                     RFQLFYEMRGLLASYRPDGFLLGEADVNLQKIDEMFGDDGDRLTGIFNFLLTNETFLA
                     LARQQAAPLERLCRNLPAVPPNCQWAGFLRNLDELDLERLTEAERDEVYEIFAPDEQM
                     RIYKRGIRRRLAPLLDGDRDRIVSAFSLLCALPGTPLIVYGDEIGMGENLRLEQRNSV
                     RTPMQWDASPNAGFSTADPDDLILPVIDDGPFRYQVVNIASQCSDPNSLLNQIKSIFA
                     IRKACPEIGCGTYDLLSGSHPHVLMTRFAHQGRSVITAHNLSAEPQCVPLDIGAPVMS
                     LLSDPESGYANGRVQLGRYGYLWLRERTNVATS"
     gene            complement(4328..4486)
                     /locus_tag="VFV93_05970"
     CDS             complement(4328..4486)
                     /locus_tag="VFV93_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164924.1"
                     /translation="MAILLWLAGAAVVLTVVGALLSRLFGRGASKESFKDPLNGIQGT
                     TSWWNWGP"
     gene            complement(4614..>4939)
                     /locus_tag="VFV93_05975"
     CDS             complement(4614..>4939)
                     /locus_tag="VFV93_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164925.1"
                     /translation="GMEWLSLTNGDGVSITAIVYPSRGSLDMRPMQRRAARNGNQTIA
                     TGLIAGGDISWISEETNGWPIHASWDNVRYRIELSIGNVTSRDDWNEDDLVALIYALA
                     GEAPE"
BASE COUNT          836 a         1579 c         1686 g          838 t
ORIGIN      
        1 ctgctgcggc gacgggtgac gatcgacctg cgcgggcagg ttgtcctgat caccggcggg
       61 tcgcgcgggc tggggctggc gctggccgag gcgttcgtcg ccgaaggcgc cgtgccggtc
      121 atctgcgccc gcaatccgga cgatctggag gacgcccgcc agcggctgct gcggcagggc
      181 gcggccgatg tgctggctgt gccgtgcgac gtgcgcgtcc gcgagcaggt cgagcggctg
      241 atcgcgacga cgctggagca cttcggccgg ctggacatcg tcgtcaacaa cgctgggacg
      301 atcatggtcg gcccgcttga gtcgcagacg ctggccgatt tcgaggatgg catggcgacg
      361 atgttctggg gcaccgtcta cccggcgctg gcggccctgc cgcagatgcg ccggcagggt
      421 ggcgggcgga tcgtcaacat cacctcgatc ggcggcaagg tcagcgtccc gcacctgctg
      481 tcgtattcag ccgccaagtt cgcggccgtc ggcttctcgg aagggttgcg ggccgaggtg
      541 gcgaaggacg gaatcgacgt cctgacggtc gtgccgggcc tgatgcggac cggctcacac
      601 gtcaacgcga tcttcaaggg gcggcacgaa caggagtaca cctggttcgg cctcggcgcg
      661 agcatgccgg gcttgacgat cagcgccgaa catgcggccc acgagatcgt cgccgcgatc
      721 aggcgcggtg atgccgaggt cacgctcggg ctgccggccc agttgctggc cgggttccac
      781 ggcctcttcc ccggcgtcac cagcgacctc atggggctcg tcaaccggct gctgccgtcg
      841 ccgggcggcg accgcgatcg ccacctcggc cgtgacagcg agacggccgt gaccgagtca
      901 tttctgacga ccctcggcca gcgcgccgcc gaacgctatc agccggacga gccgcgcgac
      961 cgcgtcctgc gcgagacgcc ccggccggtg tcataaacca cgccaaccat ctgctggtat
     1021 cctgttcaga ccgagccgga acggcctgag aggggagcat gtgggcaggt gtcgctggac
     1081 aatcgcggtt gccacgctgc tgctcgtgtt cccgttgctg gccgcctgcg gcgacgcgga
     1141 atccgacggc gcgctggcgg acgtggccgc gacaccgtct gaaccggtta gctcgccgac
     1201 tgagaccgtc gccacgactg ccagccaaac ggcgccgagc gccagcccga cgattgtcgc
     1261 atcggccagt cctgacgatg agatgacgcc ggtcgacttc ttcgagttga tcgacgcggc
     1321 gctcgcgggg accgacggca ttctccacac ccgcgtgacc tacacgacct tctaccaggg
     1381 acaggaagcg ccgatctgga ccgacgagag ctggatcgac gctggtcgcg gtgtcgcccg
     1441 gcgcgagttt cagctcgcgc ccgactccag cctcgatatg tctgagaaca tgacctacat
     1501 cgtcgtcgga gacaccagct actcgatcaa caacgatctc gccaccgacg gcagctaccc
     1561 ggacgtcgac acaacctcgg tccagaccga gtgcgcgacg atcgagaccc aggtgctggt
     1621 cgcgttcctc gtctgcggcc tctacccatt ccagccgagt gagccgcaac tgtcggttga
     1681 gccgaacgtg gagtacgagg gacaagcggc cctcgcgctg ctctacgaga tggcgactgc
     1741 gccaagttcc gacccggcgc caagggcaac gccggagcca cggccggagc gggtcgaggc
     1801 gcggctcttc atcgcccgcg agacatcact gccgctggcc tgggtcgtcg actggaacga
     1861 cgccggcggc gagacgctgg ggagccaggt cgcccgcttt gcgtcggagc cggtcgccgc
     1921 cgagtcgctg ccggccgatt tcttcgatcc gacctcgatc ggctacagcg acgatccgat
     1981 ggcgccgatc gactgggcag acctcgacat cccgatcctc tggctgggtg agcggttcga
     2041 gccgggtggc gacctgccgc tgctcgtgtt gcagtatgcc gagccgcagg ccaagggtca
     2101 gcccgactgg gccagcgcgc acttgcagta ctactcggcc gactattccg acgaccacct
     2161 ggccgacacc ttcgtcgaca tgctgctctg gacgccggag gcctggcagc gctactccga
     2221 cgagctgccc ggctggatgc cgttcaagtc catctgcgcc gaacgacgcg agctaccagt
     2281 cgaaagcggc gggacggccg tcatcatcct gacgcacgag atggaggagt cgtcggtacc
     2341 ccagccgcaa tcgacgccgg tcgagccaac gccggtggct gaccgccccg gctgccccga
     2401 cgcgccatac gatcgtttcg ccgcgatcgt cacctacgac gatgtcgtcg taaccgtcaa
     2461 cgtcctcgac tgcctctatt gcggcccgcg cccatacaag gacgacgcct acgacaccgt
     2521 ccccggcctc gaagcgatcg tcaacggcct ccgccgctac tgaccgattg tggaagctcg
     2581 acattaccat ccccgtaggg actggtctca gaccggtccc tacgaggttg cgacgttggt
     2641 tctctcccgc agccagaggt atccgtagcg accgagctgg accctgccgt tggcgtagcc
     2701 ggattcggga tcgctgagga gcgacatgac cggtgcgccg atgtcgagcg ggacgcactg
     2761 tggctcggcg gagaggttgt gggcggtgat gacggagcgt ccctggtggg cgaagcgggt
     2821 catcaggacg tgtgggtggc tgccgctgag caggtcgtag gtgccgcagc cgatctccgg
     2881 gcaggctttg cggatggcga agatcgattt gatttgattg aggagcgagt tggggtcact
     2941 gcactggctg gcgatgttga ctacctgata gcggaacggg ccgtcgtcga tgaccggcag
     3001 gatcaggtcg tcggggtcgg cggtcgagaa gccggcgttg ggggaggcgt cccactgcat
     3061 cggggtgcgg acgctgttgc gctgctccag ccgcaggttc tcgcccatcc cgatctcatc
     3121 gccgtagacg atcagcggcg tgccggggag cgcgcagagc aggctgaagg ccgacacgat
     3181 tcggtcccgg tcgccgtcga gcagcggcgc cagccggcgg cggatgcctc gcttgtagat
     3241 gcgcatctgc tcgtcgggcg cgaagatctc gtagacctcg tcgcgctcag cctccgtcaa
     3301 acgttcgagg tccagctcgt cgaggttgcg caggaagccg gcccactggc aattcggcgg
     3361 gacggccggc aggttccggc agagacgttc gagcggggcg gcctgctgac gggcgagggc
     3421 gaggaaggtc tcgttggtga gcaggaagtt gaagatgccg gtcagccggt cgccgtcgtc
     3481 gccgaacatc tcatcgatct tctggaggtt gacatccgcc tcgccgagca gaaagccatc
     3541 cggccgatag ctggcgagca gaccgcgcat ctcgtagaag agttggaagc ggtcgccgtt
     3601 cggcgctggc cgggcgccct tcggccggac tagataggga gccgcgtcga tgcggaaccc
     3661 gtccacaccc cgccgcagcc agaactccat gatctgcagg atctcggcgc agacgtccgg
     3721 gttgaacgtg tcgagatcgg gctcgaagca gtagaagcgg tgcagatagt attggccggc
     3781 catgtcgtcg tattcccaga cactgtcttc agcgccgggg aagatcggct tcgtgccggg
     3841 caactcgacc ggctcattgg accagacata atagtcacga tagcgggagg atgggtcacg
     3901 tcgcgcccgc tggaaccacg ggtgctggtc ggacgtgtgg ttggtgacca ggtcgatcag
     3961 cacccggaga ccgcgccggc tggcctcctc gatcaggtga tcgaagtcgg cgaagctgcc
     4021 gaagcgggga ttgatggcgc agtggtcgct gatgtcgtag ccgttgtcgg ccagcggcga
     4081 gccgtagaag ggaagtagcc agaggcagtc aacaccgagg tcggagaaat agtccagctt
     4141 gccggtcagc ccggccagat cgccgaagcc atcaccattc gagtcgcaga acgtcttgat
     4201 atccacgcca tagaagatgg cgttcttgta ccacaggtcg gtcatggcac ctcgtgcaca
     4261 gcactccacg cgagttggcc ctggagatgg ggattgcacg ttcggtacca gagaggttgc
     4321 ttcggcgcta cggtccccag ttccaccacg aggtggtgcc ttggatcccg ttgagcgggt
     4381 ccttgaagga ttccttgctc gcgccgcgac cgaagagacg tgagagcagc gcgccaacga
     4441 cagtcaggac gacggccgca cctgccagcc acagcagaat cgccattgaa gtctcccgtc
     4501 gcactgtgac aagtattgca cgacagccgc acactcccaa tccaatgcaa tccggtacgg
     4561 gcgatatcgc aatatcgccc gtaccggatt gcattggatt gggagtgtgc agatcactcc
     4621 ggcgcctcgc cggccagcgc gtagatgagg gcgacgaggt cgtcctcgtt ccagtcgtcg
     4681 cggctggtga cgttgccgat gctgagttcg atgcggtagc ggacgttgtc ccagcttgcg
     4741 tggatcggcc aaccgttggt ttcctcggat atccaggaga tgtcgccgcc agcgatcaag
     4801 ccggtcgcga tggtctgatt accgttgcgt gccgctcgcc gctgcattgg gcgcatgtcg
     4861 agtgatccgc gcgaggggta gacgatggcg gtgatgctga cgccatcgcc gttggtgagg
     4921 ctgagccatt ccatcccac
//
LOCUS       DASUGJ010000269         6027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1151586, whole genome shotgun sequence.
ACCESSION   DASUGJ010000269 DASUGJ010000000
VERSION     DASUGJ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151586"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFV93_05980"
     CDS             <1..674
                     /locus_tag="VFV93_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5164926.1"
                     /translation="WADPSSWQQVVDQLHRDGFQTVTPRLDLLSVEGDVSIVRAALDA
                     TPGEKILVAHSYGGFVASNASAGRSDIRALVYTAAFAPEQGETALGLFEGYEPSAALD
                     HLVFTGEPWNSPAYLDPAFFHEIFCADVSDAEAAVMNAAQQPTHPALLVTPSGPVGWH
                     DLPLYYAVSGQDRIIDPNLERFMAARSGATTIEFKKASHTGGFTAHAHQFTNLIERAV
                     RETSQ"
     gene            766..1752
                     /locus_tag="VFV93_05985"
     CDS             766..1752
                     /locus_tag="VFV93_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5164927.1"
                     /translation="MSTTSLSTDLIPAVVLEPAAQAVADALAAGGGPPLYTLSPKAAR
                     AVLDQAQAGDIPMAPATIEERTLPVGPSGQVSITIVRPAGADGMLPVVIYIHGGGWVL
                     GNFATHERLVRDLAQQTGAAYVFVNYTPSPEAHYPVAIEEAHAAMTWVAEHGAELGLD
                     GTCMAVAGDSVGGNMTAAVTLLAKERGGPALRYQAMFYPVTHATFDTDSYEQFAEGPW
                     LTRKAMQWFWDQYAPDVSRRTEPTASPLLASLDQLAGLPPTLLITDEADVLRDEGEAY
                     SRKLRQAGVDVTSVRYEGIFHDFMMLNALAETNAVRAAISEAAQALKAALIC"
     gene            1946..2824
                     /locus_tag="VFV93_05990"
     CDS             1946..2824
                     /locus_tag="VFV93_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5164928.1"
                     /translation="MATTSTAIGKSITASISERERQEIEAANASGYTPVVFIHGLWLL
                     PSSWANWADFFTQAGYAPLTPDWPDDPQTVEGARAEPEILARKTLKQVADHTTEIITA
                     LDRKPAIIGHSTGGLMAQMLAGQGLSAATVAIDPGIFRGVLPLPVSVLRGVGPFLANP
                     LTRGRAITLTFDQFKYSWANALDEREAKELYDTYHVAGSGISLAQMGNANLNPWTEAM
                     VNTSNPDRGPLLIIDGEKDHTVPWAIANAAYKRQRRNPAVTEIVKIPNRGHSLTIDHG
                     WREVAQTALDFIKRFA"
     gene            2897..3466
                     /locus_tag="VFV93_05995"
     CDS             2897..3466
                     /locus_tag="VFV93_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5164929.1"
                     /translation="MNTATTTTAGSTAVGTSWTIDSAHSSIEFSVRHMMIATVKGAFP
                     DVDGSIHWDGEHVENAAVEVRIDTRSITTHNALRDDDLRSEHFFAVEQWPTMTFTSTH
                     VEPTGRDQFTVTGDLTIRDQTRPVVLAVEYQGQITDTGGKQRAAFSATTSLSRKDFGI
                     SWNERLDGGGVVVGDTIKVMLDIAAVRQG"
     gene            complement(3586..4188)
                     /locus_tag="VFV93_06000"
     CDS             complement(3586..4188)
                     /locus_tag="VFV93_06000"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5164930.1"
                     /translation="MSEPDAGNPIVMIRGDKVGLGPIRRDLITTYQRWFNELRVTRTL
                     DLPSRPITFEDETSWFESAATGATVFFTIYELATMRPIGNLDLRNIDHYNGTAEYGIA
                     IGEIDAWGKGYGTEATRLMLAYAFDVLGLHNVQLAVYTTNPGGQRAYERAGFKRSGIR
                     RGGIRVGRERYDIIYMDATADDIQPGELHAIMHPGIAGAD"
     gene            complement(4311..5957)
                     /locus_tag="VFV93_06005"
     CDS             complement(4311..5957)
                     /locus_tag="VFV93_06005"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX5164931.1"
                     /translation="MHPHMIHHTGDPATFDRDAAGLPVAGPTRVVDLADGDHVALEAA
                     PLRKTIGTAELRLLGYNGSIPGPTLRVRQGSQVSVTFANRTDLENTVHWHGLRVENRF
                     DGTHQTQAPVPVGDSFTYSVRFPDPGVYWYHPHIREDYGQELGLYGAVLVTPTEADYW
                     PAVHREVVFTLDDILIEDDQVAPFHRAETTHAAMGRFGNVLLVNGEPNPSFSAQRGEV
                     VRYYLINTANTRVFNVGFTAARMKLVGGDSGRVEREAFVESVLLAPSERIVVDVLFDT
                     PGPVVLEQRTPERSTALATIAVAAEAAQPFLEGLFNELRTNPELVAARERMTPFIAAA
                     PDKTLAFVAELQLVSEEGASSYACPMHPEIVSATPGRCPICGMTLLPASLVATPAAHA
                     THHDMSAHAGHHGHVQDTQPPTDGIEWEDDMIEVNRQTTSANTRWKLVDRATGDEGHA
                     IDWQFKVGDQVKLRLVNELDSDHPMHHPFHIHGAGRFLVLSRDGSIEPNLVWKDTVLV
                     RKGETVDILLDITNPGIWMAHCHIAEHHESGMMLSFRVTE"
BASE COUNT         1064 a         1980 c         1927 g         1056 t
ORIGIN      
        1 cctgggccga tccgtcgagt tggcagcagg tcgtcgatca gcttcaccgg gatggctttc
       61 agacggtgac cccgcgactc gacttgctct cggtcgaggg ggatgtatcc atcgtccggg
      121 ccgcgctgga cgccactcct ggtgagaaga tcctcgtcgc ccactcctat ggcgggttcg
      181 tcgcctcgaa cgcatcggcc ggacgctccg acatccgtgc cctcgtctac acggcggcct
      241 tcgctccgga gcaaggtgag acggccctgg ggctcttcga gggctatgag ccatcggcgg
      301 cactcgacca cctcgtcttc accggtgagc cgtggaactc acccgcgtac ctcgatccgg
      361 ccttcttcca tgagatcttc tgtgccgacg tctcggatgc cgaagcagcg gtcatgaacg
      421 ccgcccagca gcccacgcac ccggcgctgc tggtcacgcc gtctggaccc gttggctggc
      481 acgatctccc gctctactac gcggtctccg gccaggatcg tatcatcgac ccgaaccttg
      541 agcgctttat ggccgcgcgc tccggcgcga cgacgatcga gttcaagaag gcgagccata
      601 ccggcggctt caccgcccac gcgcaccagt tcaccaacct catcgaacgg gccgttcgcg
      661 agacgagcca gtgaccttca tgtgtccgac cggctcttcg cacctttggc ggggccggtc
      721 actcaccagc cattgaaacg cattacccag aagaaggaga tcaccatgag caccacgagt
      781 ctgtcgactg atctgattcc ggccgtcgtt ctggagcctg ccgcgcaggc cgtcgccgat
      841 gccctggcgg ccggcggagg tccaccgttg tacaccctct cccccaaagc tgcccgcgcc
      901 gtccttgacc aggcgcaagc tggtgacatc ccgatggctc cggccacgat cgaggaacgc
      961 acgctcccgg tcggccccag tggtcaggtg tcgatcacca tcgtccggcc cgccggcgca
     1021 gacgggatgc tgcccgtggt catctacatc cacggtggag gctgggtgct gggcaacttc
     1081 gccacgcacg agcgcctcgt ccgcgacctg gcccagcaga cgggtgcagc ctacgtcttc
     1141 gtcaattaca ccccgtcacc cgaggcgcac taccccgtcg ccatcgaaga agcacacgcc
     1201 gcgatgacgt gggtggccga gcatggggcc gagttggggc tcgacgggac ctgcatggct
     1261 gtcgctggcg acagcgtcgg cggcaacatg actgcggctg tgacactgct ggccaaggaa
     1321 cgtggcggcc cggcgctgcg ctatcaggcg atgttctacc ccgtcaccca tgccaccttc
     1381 gacacggaca gctacgagca gtttgccgag ggaccgtggc tcacccgcaa ggcgatgcaa
     1441 tggttctggg atcagtacgc ccctgacgtg tcgcgtcgca ctgaaccgac cgcgtcgccg
     1501 ttgctcgcgt cactcgatca actggcggga ctgccgccca cactcctgat caccgacgaa
     1561 gcggacgtgt tgcgcgacga gggcgaagcc tacagtcgca agttgcgaca ggcgggcgtt
     1621 gacgtgacct cggtgcgcta cgaaggcatc ttccacgact tcatgatgct caacgcgctg
     1681 gcagagacca acgcggtccg cgccgccatc agcgaggccg cacaggcact gaaagccgcg
     1741 ttgatctgct agttcccgcc gccggcgatc caacagcggc ctcatcatcg cggatcgagg
     1801 ctgctgttgg aagggtacgt cagacgtact ccggtccggc ggatcgtcga gcttcatgtc
     1861 gccacgcgcg cccgagggaa acatgtcgat cggaacagac agagacggtg tcgatacttc
     1921 aatcactcat gagaggataa cgaccatggc cacaacaagc actgcgatcg ggaagtccat
     1981 cacggcgtcg atttccgagc gagagcgtca ggagatcgag gcggctaacg cctccggcta
     2041 caccccggtc gtcttcatcc acggtctctg gctgctgccg agcagttggg ccaactgggc
     2101 cgatttcttc acgcaggccg gctacgcgcc gttgacgcca gactggccgg acgatccgca
     2161 aacggttgaa ggggcccgcg cagagccgga gatcctcgcc aggaagaccc tgaagcaggt
     2221 cgccgatcac accaccgaga tcatcactgc tctggaccgg aagccagcca tcatcgggca
     2281 ctcgaccggc ggcttgatgg cgcagatgct cgcgggccag ggcctctcgg cagccacggt
     2341 ggcgatcgac cccgggatct tccgcggcgt tctgccgctg ccggtctcgg ttctcagggg
     2401 cgttggcccg ttcctggcca atccgctcac gcgcggccgc gcgatcacgc tcacgttcga
     2461 ccagttcaag tacagctggg cgaatgcgct ggacgagcgg gaggcaaagg aactctacga
     2521 cacgtaccac gtcgccggtt cggggatctc gctcgcgcag atgggcaacg cgaacctcaa
     2581 cccgtggacg gaggcgatgg tgaacacgtc gaatcccgat cgtggtccac tgttgattat
     2641 cgacggcgag aaggaccaca cggtgccgtg ggcgatcgcg aatgccgcct acaagcggca
     2701 gcggcgcaat cccgccgtga cagagattgt gaagatcccc aaccgaggcc actccctcac
     2761 gatcgaccac ggctggcgcg aggtcgccca gacggcgctc gacttcatca agcggttcgc
     2821 ctgagcgatc gggtgcggtg cctcgacaga cagcgaaaga atcgatccat cgttcaccaa
     2881 ggaagggaca gccaccatga acacggcaac cacgacgacc gctggcagca ccgcggtcgg
     2941 cacgtcctgg acgatcgaca gcgcccactc aagcatcgag ttctcagtcc gacacatgat
     3001 gatcgcgact gtgaagggcg ccttcccgga cgtcgacggt tcgatccact gggatggcga
     3061 gcacgtcgag aacgcggccg ttgaggtccg gatcgacacg cgtagcatca cgacccacaa
     3121 cgcgttgcgc gacgacgatc tacgctccga gcacttcttc gcggtcgagc agtggccgac
     3181 gatgaccttc accagcacgc acgtcgagcc aaccggccgg gaccagttca cggtcacggg
     3241 agacctcacc atccgcgacc agacgcgacc ggtcgtcctg gcggtcgagt atcagggaca
     3301 gatcaccgat accggaggga agcagcgcgc ggccttctcc gcgacgacca gcctgagccg
     3361 caaggacttc ggcatcagct ggaatgagcg gctggatggc gggggcgtcg tcgtcggcga
     3421 caccatcaag gtcatgctgg acatcgcggc cgtccgccag gggtgaccga ctcgggcaga
     3481 ttgctggctg cctgcggcga cgctgatcac ccgactcctc ccccacgcag gagccgggtg
     3541 atcagcatct cccgatgcag atgccgctgc tggcagcgcc ggtcatcagt ccgcgccagc
     3601 gatgcctgga tgcatgatgg cgtgtagctc acctggctgg atatcgtcag cggtggcatc
     3661 catgtagatg atgtcgtagc gttcgcggcc gactcggata ccgccccgac ggatgccaga
     3721 gcgcttgaag ccggcgcgct cgtaggcgcg ctggccgccg gggttggtgg tgtagaccgc
     3781 cagctggacg ttgtggaggc cgagcacgtc gaaggcgtag gcgagcatga gccgggtcgc
     3841 ttccgtgccg tagcccttgc cccaggcgtc gatttcgcca atcgcgatcc cgtactcggc
     3901 cgtgccgttg tagtggtcga tgttacgcag atcgaggttg ccgatcggac gcatcgtggc
     3961 cagctcgtag atggtgaaga acaccgtcgc tccggtcgcc gcgctctcaa accagctggt
     4021 ctcgtcttca aacgtgatcg gacgactcgg caggtccagc gtgcgcgtca cccgcagctc
     4081 gttgaaccag cgctgatagg tggtaatcag atcgcgccga atgggaccga gtccgacctt
     4141 gtcaccgcgg atcatcacga tcgggttgcc ggcgtctggc tcggacacct ggagcctcct
     4201 tcgcatgggt agcatagaga tcatgccgga gcgggatgcc tcgctgtgaa gcgagaggat
     4261 gcgggatcat ggtagccgag gcagcaggtg actgctgcct cgggggtgcg ttactcggtc
     4321 acccggaatg agagcatcat gccgctctcg tgatgctcgg cgatgtggca gtgggccatc
     4381 cagatgccgg ggttggtgat atccagcagg atgtcgaccg tctccccctt gcgcaccagt
     4441 accgtgtcct tccagaccag gttcggctcg atgctgccgt cccggctgag caccagaaat
     4501 cgaccggcac cgtgaatatg gaaggggtga tgcatggggt ggtcggagtc cagctcgttc
     4561 accagccgca gcttgacctg atcgcccacc ttgaactgcc aatcgatcgc gtgcccttcg
     4621 tcacccgtcg cgcggtcaac gagcttccag cgcgtgttgg ccgacgttgt ctggcggttg
     4681 acctcgatca tgtcgtcttc ccactcgatt ccatctgtgg gcggctgggt gtcctggacg
     4741 tggccgtggt gtccagcatg cgcgctcata tcgtggtgag tggcgtgcgc tgccggggtg
     4801 gcgaccagcg atgccggcag gagcgtcatg ccgcagatcg ggcagcgccc gggtgtcgcg
     4861 ctgacgatct ccgggtgcat cgggcaagcg tagctggatg caccttcctc agataccagc
     4921 tgcaactcgg cgacgaaggc cagcgtctta tccggcgcgg cggcgatgaa cggcgtcatt
     4981 cgctctctgg cggctacgag ctcaggattc gtgcgcagct cgttgaacag tccttccagg
     5041 aagggttgcg ccgcttccgc agcgactgca atcgtggcga gcgccgtcga gcgctccggc
     5101 gtgcgctgct cgaggacgac cggaccgggt gtgtcaaaca gcacatcgac gacgattcgt
     5161 tcggacggcg ccagcagcac gctctcgacg aatgcctcgc gctccactcg cccgctatcg
     5221 ccgccgacca gcttcatgcg ggcggcggtg aagcccacgt tgaacacccg ggtgttggcg
     5281 gtgttgatca ggtagtagcg caccacctct ccgcgctgtg cgctgaacga tgggttcggc
     5341 tctccattga ccagcagcac gttgccgaag cggcccattg ccgcgtgcgt cgtctccgca
     5401 cggtggaatg gtgcgacctg gtcatcttcg atcaggatgt catccagcgt gaacacgacc
     5461 tcgcggtgca cagccggcca gtagtcagcc tctgtcgggg taaccagcac ggcgccgtac
     5521 agcccgagct cctggccgta gtcttcacgg atatgcgggt ggtaccagta gacaccaggg
     5581 tcggggaatc gcacgctgta cgtgaagctg tcgcccaccg gcaccggggc ctgcgtctgg
     5641 tgggtcccgt caaatcggtt ttccacccgt aggccatgcc agtgcaccgt attctccaga
     5701 tcggtccgat tggcaaaggt gaccgacact tgcgagccct gacgaacgcg caacgttggg
     5761 cccgggatgg agccgttata gcccagcagc cgcagctcgg cagtgccgat cgttttgcgg
     5821 agcggggcgg cctccagcgc aacgtgatcg ccgtcggcga ggtcgaccac ccgagttgga
     5881 ccggcaaccg gcagcccagc ggcatcccga tcgaaggtcg ccggatcgcc agtgtggtgg
     5941 atcatgtgtg ggtgcatctg gtgcctcctt tgcgttctct gacgccagcg tcgctgcctg
     6001 tcgagcgacg catgatacct gtctgac
//
LOCUS       DASUGJ010000270         4222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1013720, whole genome shotgun sequence.
ACCESSION   DASUGJ010000270 DASUGJ010000000
VERSION     DASUGJ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013720"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2191
                     /gene="ppk1"
                     /locus_tag="VFV93_06010"
     CDS             <1..2191
                     /gene="ppk1"
                     /locus_tag="VFV93_06010"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX5164932.1"
                     /translation="RKQTYKGNDVPQKSKHRRTSRRERVGSGPLLAPELYLNRELSWL
                     EFNRRVLEEALDTRNPLLERVKFLSIFSSNLDEFFMVRVAGIVGQVRAGDDSVGRDGT
                     RPSEQVAAIKLLVSDLSACQRECWNTIIKPALRDEGILIEEYDGLSSRDRRGLTRYFE
                     RQIFPVLTPLAVDAGHPFPHISNLSLNMLILIEDERGEHIARLKIPSTLPRFIQIPEP
                     DGTITVAPRRKLRYVVLEDVIEANVDQLFPGKQIKSTHLFRVTRDADIEVVTGSGDSL
                     LKTIEDELEQRLFGYVVRLTVEPSMPEDTRQWLADKLNAAPDNVYVLSRPHGLSDLMQ
                     LYSIDQPALKDKPFIPRYPSVLRESDSIFSAIRNGDVLLYHPYDSFTPVVDFVRQAAA
                     DTDVVALKQTLYRVGNHSPIVDALLEARDDDTQIAVLLELRARFDEESNINWARALES
                     EGVHVAYGLAGIKTHCKIAMAVRREGDTLRRYVHLGTGNYNVATSRTYTDIGVMTCDE
                     DIAADVSDVFNYLTGYSAQSEYRKLLVAPVNLRSEMLNLIEREMSYGEKGRIIMKMNS
                     LSDYQMAEALYRAAQAGVKVDLIVRGICVLRPGVEGISENIRVVSIVGRFLEHVRLFY
                     FKHGRGDNGEAMYAGSADLMRRNLDYRVEVLFPVEDARIRGWLRDGILELQLRDNVRA
                     RLLQRDGSYVRLTPKDGQEPIDSQEFHLSYEPSAAAQAFIDARREPA"
     gene            2188..2376
                     /locus_tag="VFV93_06015"
     CDS             2188..2376
                     /locus_tag="VFV93_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164933.1"
                     /translation="MTDDEQIQRSRLALRVSQLWGEERATLNAGLIESAAAALARIDA
                     LRPEATIQLDGDEEAIDA"
     gene            2369..3658
                     /locus_tag="VFV93_06020"
     CDS             2369..3658
                     /locus_tag="VFV93_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5164934.1"
                     /translation="MPDVIAAVERSLDAIQRSESRVRAWVHVDPDQALAQATGIDLRV
                     ADGKAPPLAGLTVGVKDIIDVAGMPTVAGFEPFADQIAETDSAIAARLRTGGATIIGK
                     TTTTQFAVADPTDTRNPWNLDRTPAGSSSGSAAAVAAGHVDLAIGSQTAGSTLRPAAF
                     CGIVGFKPSFGWIPRTGMIPLADTLDTVGLMARSVVQIARLFDALADEGLDGAGDCQP
                     EAPARIGLWADATELATEGVRQLVDASLTRAIEAGAVVEEARSPAAYRDLLAMHHITM
                     LVDGAASHQKLISRHPDAYGPRVRSFVETGMAIPAHSYVRAQILRREARDRALTAWGE
                     FDLIALPTAETPAPGLESTGSPALQAVVTMFGLPSVSLPVGLSPDGLPVGLQLVAPTR
                     LDNARLLRIARWLEGLLEPLPAIPTGIADAADGTNRR"
     gene            3859..>4222
                     /locus_tag="VFV93_06025"
     CDS             3859..>4222
                     /locus_tag="VFV93_06025"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX5164935.1"
                     /translation="MLELSIKEARRLMLGAQLLAGKPPKRPTKQTMRDTIRHLGALQI
                     DSISVVARSHHIVLWSRLGNHPPEWLDQLLGQDRSLFEYWAHAAAFVPIEHYPFFRRM
                     MLSFHDGSGTRWSERSREW"
BASE COUNT          772 a         1413 c         1332 g          705 t
ORIGIN      
        1 acgcaaacag acctacaaag gtaacgacgt gccacagaaa tcgaagcacc gccggacatc
       61 ccgtcgcgaa cgagtgggct ctggcccgtt gctggcgccg gaactctatc tgaaccgtga
      121 gctcagctgg cttgagttca accggcgcgt gctcgaagag gcgctggata ctcgcaaccc
      181 gctgcttgag cgggtcaagt tcctctcgat cttctcgtcg aacctcgatg agttcttcat
      241 ggtgcgggta gccgggatcg tcggtcaggt ccgggccggt gacgacagcg tcggacgcga
      301 cggcacgcgg ccgtccgagc aggtagcggc catcaagctg ctggtcagtg atctgtcggc
      361 gtgccagcgc gagtgctgga acaccatcat caagcccgcg ctacgtgatg agggcatcct
      421 catcgaggag tacgacggcc tgtcgtcccg cgaccgtcgg ggattgacgc gctacttcga
      481 gcgccagatc ttcccggtcc tgacgccgct ggccgttgac gccggccacc cattcccgca
      541 catctccaac ctgagcctca acatgctgat cttgatcgag gatgaacgcg gcgagcacat
      601 cgcacggttg aagatcccat cgacactgcc acgtttcatc cagatccccg aacccgatgg
      661 aacgatcacc gtcgcaccgc gccgcaagtt gcgatatgtc gtgctagaag atgtcatcga
      721 ggcgaacgtc gatcagctct ttcccggcaa gcagatcaag agcacgcacc tgttccgcgt
      781 tacccgcgat gccgatatcg aggtcgtgac cggcagcggc gacagtttgc tcaagacgat
      841 cgaggacgag ctggagcagc ggctgttcgg ctatgtcgtc cggctaacgg tcgagccgtc
      901 gatgccggaa gatacccgcc agtggctcgc cgacaagctg aatgccgcgc cggacaacgt
      961 ttacgtcctc tcgcgcccac acggcctgtc cgacttgatg caattgtatt ctatcgatca
     1021 gccggcgctg aaagacaagc cgttcatccc gcgctaccca tccgtcctgc gcgagagcga
     1081 ttcgatcttc tcggcgattc ggaacggcga tgtcctgctc tatcacccgt acgactcgtt
     1141 tacgccggtg gtcgacttcg tccgtcaggc ggccgctgac acggatgtcg tcgccctcaa
     1201 gcagacgctc tatcgggtcg gtaatcactc gccgatcgtc gacgcgctac tcgaagcccg
     1261 cgatgacgac acccagatcg ccgtcctgct ggagctgcgg gcgcggttcg acgaggagag
     1321 caacatcaac tgggcgaggg cgttggaaag cgagggcgtc catgtcgcct acggcctggc
     1381 cggtatcaag acacactgca agatcgcgat ggcggttcgt cgcgagggcg acacactgcg
     1441 acggtacgtc catctcggga ccggcaacta caacgtcgcc acctcgcgca catacaccga
     1501 tatcggcgtg atgacctgcg acgaggacat cgccgccgat gtctcggatg tcttcaacta
     1561 cctgaccggc tactcggcgc agtcggagta tcgcaagctg ctggtcgcgc cggtcaacct
     1621 gcgctctgag atgctcaacc tgatcgagcg cgagatgtcc tacggcgaga agggcaggat
     1681 catcatgaag atgaactccc tctccgacta ccagatggcc gaggcgctct accgggctgc
     1741 tcaggccggt gtcaaggtcg atctgatcgt gcgcggcatc tgcgtcctgc ggcccggtgt
     1801 cgagggcatc agcgagaaca tccgcgttgt cagcatcgtc ggccgctttc tggagcatgt
     1861 gcgcctgttt tacttcaagc acgggcgcgg cgataatggc gaagcgatgt acgcagggag
     1921 cgccgacctg atgcgccgca acctcgatta ccgtgtcgag gtgctcttcc cggtcgagga
     1981 cgcgcgcatc cgtggctggc tgcgcgacgg catcctcgaa ctacaactgc gcgataacgt
     2041 ccgcgcccga ctcctgcaac gcgacggatc gtacgtccgg ttgacgccca aggacgggca
     2101 ggaaccgatc gactcgcagg agttccacct cagttacgaa cccagcgccg ccgcacaggc
     2161 attcatcgat gcacgacggg agccggcatg accgacgacg agcagatcca acgatcccgg
     2221 cttgccctgc gggtcagcca gctctggggc gaggagcggg caacgctcaa tgccgggctg
     2281 atcgaatccg cggcagctgc cctggcccgc atcgacgcct tgcggccaga ggcgaccatc
     2341 caactcgacg gcgacgagga agcgatcgat gcctgacgta atcgcggcgg tagaacgctc
     2401 gctcgacgcc atccagcgta gcgaatcgcg cgttcgggcc tgggtacatg tcgatcccga
     2461 tcaggcactg gcacaggcga ccggcatcga tctgcgcgtc gcggatggca aggctccgcc
     2521 gctggcgggg ctgacggtgg gcgtcaagga catcatcgat gtggcgggaa tgccgacggt
     2581 agccggtttc gagccgttcg ccgaccagat cgcagagacc gactcggcaa tcgcggccag
     2641 actgcgaaca ggcggcgcaa cgattatcgg caagacgacg acaacccagt tcgccgtcgc
     2701 cgacccgacc gacacgcgca acccgtggaa cctcgatcga acgccggccg gctcgagctc
     2761 cgggtcagcg gcagccgtcg cggccgggca cgtcgatctg gcgatcggct cgcagacggc
     2821 aggttcgacg ctgcggccag ccgcgttctg cggcatcgtc ggcttcaagc cctcgttcgg
     2881 ctggatcccg cgaaccggga tgatcccgct ggccgacacg ctcgacaccg tcggcctgat
     2941 ggcgcgcagc gtcgtgcaga tcgcgcggct gttcgacgcg ctggccgatg aggggctgga
     3001 cggcgccggc gactgccagc cggaggcgcc ggcgcggatc ggactctggg cagacgcaac
     3061 agagttggcg acggaaggcg tgcgtcagct ggtcgacgcc tccctgaccc gcgcgatcga
     3121 ggccggcgcc gtcgtcgagg aggcgcgatc accggccgcc taccgcgatt tgctggcaat
     3181 gcatcacatc acgatgctgg tcgacggggc cgcgtcccac cagaagctca tctcccgaca
     3241 tccggacgcc tacggccccc gcgtgcgctc gttcgtcgag accgggatgg cgattcccgc
     3301 ccacagctat gtgcgcgccc agatcctgcg acgcgaggcc cgcgaccgcg cgctgacggc
     3361 ctggggcgag ttcgacctga tcgcgctacc aaccgccgag acaccggcgc ccggtctcga
     3421 atcgaccggc agcccggcgc tccaggcggt cgtgaccatg ttcgggcttc catccgtctc
     3481 gctgccggtc ggtctatcgc ctgacggcct gccggtcggc ctccaactgg tcgccccgac
     3541 ccgactcgac aacgcccgcc tgctgcggat cgcgcgctgg ctcgaagggc ttctcgaacc
     3601 attgccggcc atcccgaccg ggatcgcgga cgcagctgac ggcaccaacc gccggtaacg
     3661 cgtgacgcgt gatgcgcccc agagggacgt cccgcattac gtaaccggtg gggccgggac
     3721 ggtgccacgg cccccaaccg aaagaagtca ccatcgcttc cagcggctgc agatgccaac
     3781 cgcacggatg ttctactctc tgctacaatc agccttctgc atggtggtgt acggacatga
     3841 tcgggaggtc acaggacgat gctcgaactg tcgatcaaag aggctcgccg gttgatgctc
     3901 ggggcacagt tgctggccgg caagccgccg aaacggccga ccaagcagac gatgcgcgac
     3961 acgatccggc atctcggcgc actccagatc gactcgatca gcgtcgtcgc ccgtagtcac
     4021 cacatcgtcc tctggagccg gttgggcaac cacccgcccg aatggctcga ccagctgctc
     4081 ggccaggatc ggtcgctgtt cgagtactgg gcgcatgcgg cagccttcgt cccgatcgaa
     4141 cactacccgt tctttcggcg gatgatgttg agcttccacg atggctccgg cacgcgctgg
     4201 tcggagcgct ctcgcgaatg ga
//
LOCUS       DASUGJ010000271         3818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1708076, whole genome shotgun sequence.
ACCESSION   DASUGJ010000271 DASUGJ010000000
VERSION     DASUGJ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708076"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2026
                     /locus_tag="VFV93_06030"
     CDS             <1..2026
                     /locus_tag="VFV93_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5164936.1"
                     /translation="MSPQIEQMLGYPPEAWTTGDIWDEIIHPDDLEWVTRLDKETDAT
                     LAPYDAEFRVFASDGRLVWLRNYSVAVCDEDGHPRYWQGYLIDITAQKVAEEAIRFQA
                     QLLDTVGQAIIAADIDGKITYWNRAAEALYGWTSDEVRGRNVFEVTPVEQDYDWSGLM
                     ETLQDGEIWTGEFSARRKDGSIFPAFVTSSPVCDDDGTLIGIIGVSTDISERKQLEEQ
                     LLHQAFHDALTGLPNRNLFLDRTRHALSLARRHDELVAVLFLDLDNFKIVNDSLGHDI
                     GDQLLVTIAGRILGCLREGDTAARLGGDEFAILLENLGNSEDVAVVAQRLLDALRTPL
                     LLDGHEMFVTPSIGIAVSDTSADLAEDLLRDADVAMYIAKRNGRARYEIFEPSMGSMA
                     RQRLELEHDLRRALDLNELALHFQPVVNLGDGSLAGFEALLRWYHPKRGWIAPDVFVP
                     VAEETGLIVPVGSWALREACRQMRAWQDVWPEAARLSISVNLSGRQFKEPGLADDVAR
                     VLRETGLTPSCLNLEITETIMMADEEGTSAVLRQLEQLGVTLAIDDFGTGYSSLGTLR
                     HFPVNTLKIDRSFVDGLGAESDDSVIVSGVIGLAHGLGLRVVAEGVETTDQLARLHEL
                     GCDLGQGYYFSRPLPPDEVFAVRYRLTAGWPVRVEMAHYPLTDHALTPGS"
     gene            2064..2633
                     /locus_tag="VFV93_06035"
     CDS             2064..2633
                     /locus_tag="VFV93_06035"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5164937.1"
                     /translation="MVLPDDFRFEIPRLETPRLVLRRIVPADAEDVFAYASDPEVAAW
                     TTWEAHRTLDDSREFIGRVQIWYADGFGGPWGLELKETGQLIGTCGMAITPHHGRAEL
                     GYALGRRWWGQGLTTEAVVEAIRYGFDDLGLNRIEARCIPSNLGSARVMEKAGMTCEG
                     TLRQQFYAKGSFVDLKMYSILRHEWLPAR"
     gene            2711..3082
                     /locus_tag="VFV93_06040"
     CDS             2711..3082
                     /locus_tag="VFV93_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164938.1"
                     /translation="MLSPEEVVAEYGASLIEPDEARRLVILAGVWTDDCEVILPEVRL
                     VGREAINAHITTIRRAVGRATPILSGPVDAHNGFLRFEWRMIDAAGEVVAAGVNIGEQ
                     AADGRLRRVVLFRGVRPGVYL"
     gene            3103..>3818
                     /locus_tag="VFV93_06045"
     CDS             3103..>3818
                     /locus_tag="VFV93_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5164939.1"
                     /translation="MAETQRPAKIGMLLPHLEGSYLGGTARWADLAAMAKTAEDVGFD
                     SLWVADHLLYRFPDVPEFGVWECWSLMAGLAAVTNRVELGTIVSVTPWRNPALFAKMV
                     DTVEEISGGRVILGLGAGSHQPEFPAFGYDSWDHKVSRFEEEIGIIHTLLRTGQIDHE
                     GTYHTLRDCELRPRGPRPEGPPIMVGAMGPRMLRILATYADEWNIPWRQTVEDLLEVN
                     PRADEACAKVGRDPATLRRSA"
BASE COUNT          627 a         1225 c         1321 g          645 t
ORIGIN      
        1 catgagcccg cagatcgagc agatgctggg ctacccgccc gaagcgtgga ccaccggcga
       61 catctgggat gagatcatcc atcccgatga cctggagtgg gtgacccggc tcgacaaaga
      121 gacggatgcg acgctggcgc cgtacgacgc cgagttccgc gtcttcgcca gcgatggccg
      181 gctggtctgg ctgcgtaact actcggtcgc cgtctgcgat gaggacggcc acccgcgcta
      241 ctggcagggc tatctgatcg acatcaccgc ccagaaggtt gccgaggaag cgatccgctt
      301 ccaggcccag ttgctcgaca cggttggtca ggccatcatc gcggccgaca tcgacgggaa
      361 gatcacctac tggaaccgcg ccgccgaagc cctgtatggc tggacgtccg acgaagtgcg
      421 cggtcgcaat gtctttgagg tcacgccggt cgagcaggac tacgactggt ccgggctgat
      481 ggagacgttg caggacggcg aaatctggac cggcgagttc tcggcgcggc gtaaggacgg
      541 cagcatcttc ccagccttcg tcaccagttc gccggtctgc gacgacgacg gcacgctgat
      601 cgggatcatc ggcgtctcga ccgacatctc ggagcgcaag cagcttgagg agcagcttct
      661 ccatcaggca ttccacgacg cgttgaccgg gctgccgaac cgcaacctgt tcctcgaccg
      721 tacccggcac gcgctctcgc tggcgcggcg gcacgacgag ctggtggcgg tcctcttcct
      781 cgatcttgac aacttcaaga tcgtcaacga cagcctcggc cacgatatcg gcgatcagtt
      841 gctggtgacg atcgccgggc ggattctcgg ctgcctgcgc gagggcgaca cggccgcccg
      901 gctcggcggc gacgagttcg cgatcctgct cgaaaatctc ggcaactccg aggatgtcgc
      961 cgtcgtcgcc caacgattgc tggatgcgct gcggacgccg ctcctgctgg acggtcacga
     1021 gatgttcgtc acgcccagca tcgggatcgc cgtcagcgac accagcgccg atctggccga
     1081 ggatctgttg cgcgatgcag atgtcgcaat gtacatcgcc aagcgcaacg gacgggcacg
     1141 ctacgagatc ttcgagccgt cgatgggctc gatggcccgc cagcggcttg aactggagca
     1201 cgacctgcgg cgggcgctcg atctcaacga actggcgttg cacttccagc cggtcgtgaa
     1261 tctcggcgac ggctcgctgg ccggcttcga ggcgttgctg cgctggtacc atccaaagcg
     1321 gggctggatt gcgccggacg tgttcgtgcc ggtcgccgaa gagacgggcc tgatcgtgcc
     1381 ggtcggctcc tgggcgctgc gcgaggcttg ccggcagatg cgcgcctggc aggacgtgtg
     1441 gccggaggcc gcgcggctca gcatcagcgt caacctgtca ggacggcagt tcaaggagcc
     1501 ggggctggcg gacgacgtcg cgcgggtgct gcgcgagacc ggcctgacgc cgtcgtgcct
     1561 gaatctggag atcaccgaga cgatcatgat ggctgacgaa gagggcacca gcgccgttct
     1621 gcgccagctg gagcagctcg gtgtgacgct ggcgatcgac gacttcggta ccggctactc
     1681 gtcgctggga acgctgcgcc actttccggt caacacgctc aagatcgacc gctcgttcgt
     1741 cgatggcctc ggcgccgagt cggacgacag cgtcatcgtc tcgggcgtga tcgggctggc
     1801 tcacggcctc ggtctgcggg tcgtcgccga aggcgtcgag acgaccgacc agctcgctcg
     1861 cctgcatgag ctcggctgcg acctcggcca gggctactac ttctcgcggc cacttccgcc
     1921 ggacgaggtg ttcgccgtcc gctaccgcct gaccgccggc tggccggtgc gggtcgagat
     1981 ggcacactac ccactgaccg accacgcctt gacgcccggt tcctgacgcc tgaccacctg
     2041 ctcgtcacca atggagatgc gcgatggtgc tgcccgacga cttccggttc gagattccgc
     2101 gactcgaaac gccccgactc gtgctgcgac ggatcgtgcc ggccgatgcg gaggacgttt
     2161 tcgcctacgc ctccgacccg gaggtggcgg cctggacgac ctgggaagcg caccgcacgc
     2221 tcgatgactc gcgtgagttc ataggacgcg tccagatctg gtatgccgac ggcttcggcg
     2281 gtccctgggg tttggaactg aaggagaccg gccagttgat cggcacctgt gggatggcga
     2341 tcacgccgca ccacggccgc gccgaactgg gctacgcgct cggccgccgc tggtgggggc
     2401 aggggctgac gaccgaggcg gtcgttgagg cgatccgcta cggcttcgac gacctcggcc
     2461 tcaaccggat cgaagcccgc tgtattccgt ccaacctcgg ttcggcccgc gttatggaga
     2521 aggccggcat gacctgtgaa ggcacgctcc gccagcagtt ctacgccaaa ggcagcttcg
     2581 tcgatctgaa gatgtactcg atcctgcggc acgagtggct accggcccgc taacagaagc
     2641 gtaagcagcc cgcgagacgg tagactgcac cgggagtggg atgttgcgtg gctgaagggg
     2701 agcgggtggg gtgctgtcgc cggaggaggt cgttgccgaa tacggggcca gcctgatcga
     2761 gccggacgag gctcggcggc tggtgattct tgccggtgtc tggacggatg actgcgaggt
     2821 gatcctgccg gaagtgcggc tggttggccg cgaggcgatc aacgcccaca tcacgacgat
     2881 ccggcgtgct gttggccggg cgacgccgat cctgtccggg ccggtcgatg cccacaatgg
     2941 attcctgcgc ttcgagtggc ggatgatcga cgcggccggc gaggttgtcg cggccggcgt
     3001 caatatcggt gagcaggcag ccgatggccg gttgcgccgc gttgtcctct ttcgcggcgt
     3061 ccggccaggc gtctatctgt agtgacgaac aggggagaat gaatggcgga aacgcaacga
     3121 ccggccaaga tcggaatgct cctgccgcat ctcgaaggct cgtacctggg tggcaccgcc
     3181 cgctgggcgg acctggcggc gatggcgaag acagccgaag acgtcggctt cgattcgctc
     3241 tgggtggctg accacctgct ctatcgcttt ccggacgttc ctgagttcgg ggtctgggag
     3301 tgctggtcgc tgatggccgg gctggcggcc gtcaccaacc gggtcgagct tggcacgatc
     3361 gtgtcggtga cgccctggcg caacccggcc ctgttcgcca agatggtcga caccgtcgag
     3421 gagatcagcg gcgggcgggt gattctcggg ctgggggccg gctcgcacca gccggagttc
     3481 cccgccttcg gctacgacag ctgggaccac aaggtcagcc gcttcgagga agagatcggc
     3541 atcatccata cgctgctgcg gaccggccag atcgaccacg aaggaacgta ccacacgctg
     3601 cgcgactgcg aactgcgccc gcgcggcccg cgtccggagg ggccgccgat catggtcggc
     3661 gcgatggggc cacgcatgct gcggattctg gcgacctacg ccgatgagtg gaacatcccc
     3721 tggcggcaga cggtcgagga tctgctggag gtcaacccgc gcgccgacga ggcctgcgcc
     3781 aaggtcggtc gcgacccggc cacgctacgg cgctcggc
//
LOCUS       DASUGJ010000272         7409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_735313, whole genome shotgun sequence.
ACCESSION   DASUGJ010000272 DASUGJ010000000
VERSION     DASUGJ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735313"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFV93_06050"
     CDS             complement(<1..801)
                     /locus_tag="VFV93_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5164940.1"
                     /translation="MATGPGHSLDDLTKAAAKATQAFETRLATLAATAEQIKHDLDAT
                     RAQIQRDREEMDIEYSLETGSSYVVESPDSGDLRERERLLTEQTIRASSLQRQLAEFT
                     TLVAVSGRQLGSDGQFPGADAQTQAAIRSAGIEAQESERHRLAREIHDGPAQALANAI
                     IALEFVERAIRAGGEGSAERAQEEVERIKSTLREGLTEIRRFIFDLRPTMLQDRGLVA
                     TVEHYIATYQSLFPMAIEFHATDALSRLTVDQELTAFRVIQEAIQNARK"
     gene            993..1688
                     /locus_tag="VFV93_06055"
     CDS             993..1688
                     /locus_tag="VFV93_06055"
                     /inference="COORDINATES: protein motif:HMM:NF018952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HEX5164941.1"
                     /translation="MLGLVTIGQSPREDVVASMFSSPVQADVVEAGALDELDRQQIAA
                     LSPGPDEPPLVTRLRDGSEVVIGKKQVLPLMERAVGRLEADGCTTICVLCTGEFPRLG
                     QSALVIYPDRLLLHVVEALVPEGVLGVLMPHVGQRRGMLAKWTTPARSAVTGVASPYS
                     GSDAIASEVRRLVEAGAEAIVLDCMGFDRRMLADARSATSKPVILANGLVGGVLREVV
                     GVSAGFLDGVQRA"
     gene            complement(1653..2339)
                     /locus_tag="VFV93_06060"
     CDS             complement(1653..2339)
                     /locus_tag="VFV93_06060"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5164942.1"
                     /translation="MQGSLSEFTLAQLLQFFALAERSGTVVVKSAGRESRLLVEGDRI
                     IGWGQDDYDVREPLLTCEMISPAELQAITDVQPRSDTPGLSFVVRNLIQPERWEFFAQ
                     RQLEQDIYPLLNAEEGDFEIQVLRNPPAPLRLSIPINSMILDGSRWESEMEAAALEGY
                     QLESTWQRQHRQQASPAVQLSGLEWMVWAILTEPLSIAAIARRLCMPDLATIGAIKRL
                     RSLNTIEEAG"
     gene            complement(2391..3233)
                     /locus_tag="VFV93_06065"
     CDS             complement(2391..3233)
                     /locus_tag="VFV93_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEX5164943.1"
                     /translation="MTDERPRKLGAGDLTACPATSSGADTTLDDDQRPELTIVVPAYN
                     ESARLPRTLSDIDQFIATRPFSVELLVVDDGSDDDTCRLAQQHPSTAASYQVLRLPHR
                     GKASAVRAGVLAASGRHVLFTDADLSTPIDYATKLTDALDAGADVAIGSREGIGATRV
                     GEPSYRHVMGRVFNAVVRLLAVPGINDTQCGFKAFQREAAQAIFTRTILHAGDGVVDG
                     PRVTGFDVEVLYVARRLGYTIAEVPVYWEHVVGSKVQPLPDSFRMFADVLRVRWNAIR
                     GRYH"
     gene            complement(3226..4218)
                     /locus_tag="VFV93_06070"
     CDS             complement(3226..4218)
                     /locus_tag="VFV93_06070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5164944.1"
                     /translation="MTRVDLIIPTLNGSDWLARCLESLQRSTFTDFTLTIFDDGSTED
                     IASVVQPRFPAARIIRSEHNAGLARAFNTAITTGASEYVVLLNNDTEVEPTWLAELVA
                     CADRHPSAGSVASKLRLLSDRTRLHSAGDSWSVRGMPGNRGVWLDDFGQYDSESDVFS
                     ACGGAALYRRQALEDLLQRDGHIFDELLFMYCEDVDLGWRMQTAGWPCVFAPGAVVYH
                     ALSATGGGTLASYYVSRNVWFVMARSVPSMFLRSYRRRIAAYHLGRAWRALRHLREPA
                     ARASLRGMCAGLAAARTLRKHVRELPNTEQQRILGLLDGVDRPLPSPGHCYTHD"
     gene            complement(4215..5057)
                     /gene="rfbD"
                     /locus_tag="VFV93_06075"
     CDS             complement(4215..5057)
                     /gene="rfbD"
                     /locus_tag="VFV93_06075"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX5164945.1"
                     /translation="MASKRRVVITGTTGQLGGALAAAQRDAWEIHPLNRTDTNLLDWQ
                     AVRDRIASFAPDLVIHCAAATDVDGCERDPQVAYLGNAVATRHVARAAAACDAQLVYV
                     STNFVFDGTKPDPYHECDDPAPISVYGHSKLAGEREVLAATNRSFVVRTAMVYAATGR
                     NFVLTMRRLMADRDRLTVVNDQFGNPTYAPDLAAGIVQLVERAPFGVYHMTNAGTASW
                     YEWAREIAAIDGATTTIEPIPAAEYRRDATPPANGVLTSLSLPLLGIELPDWHDALRR
                     CLAQ"
     gene            5198..5521
                     /locus_tag="VFV93_06080"
                     /pseudo
     CDS             5198..5521
                     /locus_tag="VFV93_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
     gene            5528..6430
                     /locus_tag="VFV93_06085"
     CDS             5528..6430
                     /locus_tag="VFV93_06085"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX5164946.1"
                     /translation="MLKLGAHMSIAGGFDKAVQRAHSVESDALQIFTKNQKQWKAKPI
                     LDEDVTLFRQHCLDCVIDPVVAHDSYLINLASPDDEMWEKSIDAFRIELERCEQMGIP
                     FLVTHPGAHMKSGTEAGVARVVAALDRIHVDLPGYKVKTLLETTAGQGTTLGATFEEL
                     AALIAGVAAPERVAVCYDTCHTFVAGYDIRTPEAYAETMSKFDRIIGVERIEAFHFND
                     ALTEIGSKRDRHAHIGAGYIGAEGFRNFMNDPRFAGKPALLETEKGEDLAEDREALIL
                     LRSLVDGAGGGSAAAAPAVATPAG"
     gene            complement(6414..>7409)
                     /locus_tag="VFV93_06090"
     CDS             complement(6414..>7409)
                     /locus_tag="VFV93_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5164947.1"
                     /translation="PIAGISPFNFPLNLALHKVAPAIASGNPIVLKPPTLDPLTMLTV
                     AEMIDQLGLPKGAVSIMPMDRTVGDRLVTDERFKLLSFTGSPDVGWDMKSRAGKKKVV
                     LELGGNAGVAIDRDADIDFAVSRVRMGAFAYAGQVCISVQRVFVHQDVYNEFCEKLTA
                     AVATIKTGDPLDTETELGPMIDDKAARRIDEWVQASVKEGGRVLTGGHANGRFYEPTV
                     LVDVARESFVCSREAFAPVVTVFPTKDWDETLSKINDSVFGLQAGVFTANLEHALQAF
                     ERLDVGGVILNDVPVYRIDHMPYGGVKDSGLSREGLRYAIEDMTETRLLVVNRLA"
BASE COUNT         1291 a         2230 c         2555 g         1333 t
ORIGIN      
        1 cttgcgtgcg ttctggattg cttcctggat gacccggaac gccgtcagtt cctggtcgac
       61 ggtcagccgc gagagggcat cggttgcgtg aaactcgatc gccatcggga agagcgactg
      121 gtaggttgcg atgtagtgct cgaccgtcgc aaccaacccg cgatcctgaa gcatcgtcgg
      181 ccgtagatcg aagatgaagc gccggatctc ggtcagcccc tcgcggagcg tggacttgat
      241 gcgctcgact tcctcctggg cccgctcggc agaaccttcg ccgccagccc ggatggcccg
      301 ttcgacgaat tccagcgcga tgatcgcgtt ggccagcgcc tgggcggggc cgtcgtggat
      361 ctcgcgggcg agccggtggc gctcgctttc ctgagcctcg atgccggccg agcgaatggc
      421 cgcctgggtc tgtgcatcag cgccggggaa ctggccgtcg gagccgagct gccggccgga
      481 aacggcgacg agggtcgtga actcggccag ttgtcgctgg agcgaggacg cgcgaatcgt
      541 ctgttcggtc agcaggcgtt cgcgctcgcg cagatcgccg ctgtccggcg attcgacgac
      601 gtacgaggag ccggtttcga gtgagtactc gatatccatc tcttcgcggt cgcgctgaat
      661 ctgggcgcgg gtggcatcca ggtcatgctt gatctgctcg gctgttgcgg cgagagtcgc
      721 gaggcgggtt tcgaacgcct gagtggcctt cgccgcggcc ttggtcaggt catcgagact
      781 gtgacccggt ccggtcgcca cgaactactt cccatcgtct gccttgccgg ccaacttgca
      841 tgcgctcacg cgagcagcct tcgttgtacg acttgctgcc ccagatatca tcgaactcag
      901 gcgtcattgc gttcccatgt cactcttgcg agtataacag acgcggtttc acccaagaca
      961 gccgcgagcg atgccgctgg gaggttaacg tgatgctcgg tttggtcacg atcggtcagt
     1021 cgccccgcga agatgtggtc gcctccatgt tcagttcgcc cgtgcaggca gatgttgtcg
     1081 aagcgggagc gctggacgag ctcgacagac aacagatcgc cgcgctctca ccggggccgg
     1141 atgagccgcc gctggtgacg cgtctgcgcg acggcagcga agtggtgatc ggcaagaagc
     1201 aggtgctgcc gttgatggag cgggcggtcg ggagactcga agcggacggc tgcacgacga
     1261 tctgcgttct ctgcacgggc gagttccccc ggttggggca atcggcgctc gtcatctatc
     1321 ccgaccggct cctgttgcac gtcgtcgagg cgctcgtacc tgaaggcgta ctcggcgtgc
     1381 tgatgccaca cgtggggcaa cggcgcggga tgctggccaa gtggacgaca ccggcccggt
     1441 cggcggtaac gggggtcgcc tcgccgtata gcggatcgga cgcgattgcg agcgaggttc
     1501 ggaggctggt cgaggcagga gcggaggcga ttgtcctgga ttgcatggga ttcgaccggc
     1561 gaatgctggc tgacgcccgg tcggcgacct cgaagccggt tatcctggcc aacgggctgg
     1621 tcggcggcgt gctgcgtgag gtggtcggtg tgtcagccgg cttcctcgat ggtgttcaac
     1681 gagcgtagtc gcttgatggc gccgatcgtc gccagatccg gcatgcagag ccgccgggcg
     1741 atcgcggcga tcgagagtgg ttccgtcaag atcgcccaga ccatccactc cagcccgctc
     1801 aactggactg ccggagaggc ttgctgacga tgctggcgct gccaggtgct ctcaagctgg
     1861 tagccctcca gggctgcggc ctccatctcg gattcccagc gtgatccgtc gaggatcatc
     1921 gagttgatgg ggatcgagag gcgtagcggc gccggtggat tgcgcagcac ctgaatctcg
     1981 aaatctccct cctcggcgtt caggagcggg tagatatcct gctcaagctg tcgctgggcg
     2041 aagaactccc agcgctcggg ctggatcagg ttgcggacga cgaacgatag tcccggtgta
     2101 tccgaccggg gctggacatc ggtgatggcc tgcaactcag ccgggctgat catctcgcag
     2161 gtcagcaatg gttcgcgaac gtcgtagtcg tcctgtcccc atccgatgat ccggtcaccc
     2221 tcaaccagca gacgcgattc ccggccggcg gacttcacga cgacagttcc ggaccgttcg
     2281 gcgagcgcga agaactggag gagctgggcg agtgtgaact ccgagaggct tccctgcatc
     2341 agcgacacct tcgtctgaca accagctgac gccgacaagc gtcgctcagg ctagtggtag
     2401 cggccgcgga tcgcgttcca gcggacgcgc agcacgtctg caaacatccg aaacgagtcc
     2461 ggcaacggct ggaccttgct tccgaccaca tgctcccaat atacaggcac ttccgcgatg
     2521 gtgtaaccca gcctgcgcgc gacgtagagg acttcgacat cgaagccggt cacgcgcgga
     2581 ccgtccacaa cgccatcacc ggcatggagg atcgtccgcg tgaagatcgc ctgggcggcc
     2641 tcgcgctgga atgccttgaa tccgcattgg gtgtcgttga taccgggcac cgccaggagc
     2701 cggacgaccg cattgaagac gcgccccatc acgtgcctgt acgacggttc tccgacccgc
     2761 gtcgcgccga tgccctcgcg ggagccaatg gcgacgtccg cgccagcatc gagcgcatcg
     2821 gtcaacttcg tggcgtagtc gatcggcgtt gagaggtcgg catcggtgaa gaggacgtgt
     2881 cgcccgctgg cggccagcac accggcgcga acggcactcg ccttgccgcg atggggaagg
     2941 cgcaggacct ggtagctggc ggcggtcgat ggatgttgtt gagcgagacg gcaggtatcg
     3001 tcatcgctgc cgtcatcgac gacgaggagc tcgacgctga acggtcgggt tgcgatgaat
     3061 tggtcgatgt cgctcaacgt ccggggcagg cgcgctgact cgttgtaggc cggaacgacg
     3121 atcgtcagtt ccggtcgctg gtcgtcgtcc aacgtcgtgt cagctccaga cgaggtcgcc
     3181 gggcaggcgg tcaggtcgcc ggcgccgagc ttccgcggtc gctcatcagt catgagtgta
     3241 gcaatgtcct gggctgggaa gaggtcggtc tacgccgtca agcaggccca ggatacgttg
     3301 ttgctcggta ttagggagct cgcggacgtg tttcctgagc gttcgagctg cggccagtcc
     3361 cgcgcacatt ccacggagtg acgccctggc ggctggctcc cggagatgcc tgagtgcgcg
     3421 ccacgcgcga cccagatgat aggcggcgat gcgccggcga tacgaccgca ggaacatgct
     3481 cggaacgctt cgggccatca cgaaccagac gttgcgggag acgtagtaac tggcgagagt
     3541 gccgccgccg gtggccgaca gcgcgtggta gacgacggcg cctggggcga acacgcaggg
     3601 ccagccggcg gtctgcatgc gccagccgag gtcgacatcc tcgcagtaca tgaagaggag
     3661 ctcgtcgaag atatgtccgt cgcgctggag cagatcttcg agcgcttgcc gtcggtacag
     3721 cgcagcgccg ccgcaggccg aaaagacgtc actctcgctg tcgtactgcc cgaagtcgtc
     3781 cagccagacg ccgcggttgc cgggcatgcc gcgcaccgac cagctgtcgc cagccgagtg
     3841 aagccgcgtt cgatcggaga ggagccgcaa cttggaggca acgctgccgg ccgaagggtg
     3901 ccggtcggcg caggcgacca gctccgccag ccaggttggc tcgacctcgg tgtcgttgtt
     3961 gaggaggacg acatactcgc tcgcaccggt cgtgatggca gtgttgaacg ctctcgcaag
     4021 tccggcgttg tgctcggagc ggatgatccg ggcggcagga aagcgcggct ggacgacgct
     4081 ggcgatgtct tcggtcgatc cgtcatcgaa gatcgtcaat gtgaagtcgg tgaacgtcga
     4141 gcgttgcaga gactccaggc aacgggcaag ccagtcggag ccgttgaggg ttgggatgat
     4201 caggtcaacg cgcgtcattg ggcgagacac cgccgcagcg cgtcgtgcca gtccggcagt
     4261 tcgatgccga gtagggggag tgatagcgag gtgagaacgc cgttggccgg tggcgttgcg
     4321 tcgcggcggt attcggcggc cggaatcggc tcgatcgtcg ttgtcgcacc gtcgatggcg
     4381 gcgatctcgc gtgcccattc gtaccacgac gcggttccgg cgttggtcat gtggtagacg
     4441 ccgaacggcg cgcgctcgac gagctggacg atgccggcgg cgaggtccgg tgcataggtc
     4501 gggttaccga actggtcatt gacgaccgtc agccggtcgc gatcggccat caagcggcgc
     4561 atcgtcagca caaagttacg gccggtcgca gcgtagacca tcgcagtcct gacgacgaag
     4621 gagcggtttg tcgctgccag aacctcgcgt tctccggcca gcttggaatg gccgtagacg
     4681 ctgatcggcg ccgggtcatc gcattcgtga tagggatcgg gcttcgttcc gtcgaagacg
     4741 aaattggtcg agacgtagac gagttgcgcg tcgcacgcgg cggcggcccg cgcgacgtgt
     4801 cgggtagcga cggcgttgcc cagataggcc acctgcgggt cccgctcgca gccgtccacg
     4861 tctgtggcgg cggcgcaatg gatcacgaga tcgggcgcga aggaggcaat gcggtcgcgg
     4921 acggcctgcc agtcgagcag gttcgtgtcg gtccggttaa gtggatggat ctcccacgcg
     4981 tcccgctggg cagcggccag ggcaccgccc agctggccag tggtcccagt gatgacgact
     5041 cgtcgcttgc tcgccatgtc gtgaacgcgc ctttcacgtc ccggttgccc ggaatgacag
     5101 gcatgatgat acaatccccg aacagatgta cgtacacgtt gcgagggaga tgatgtcgat
     5161 tgttgcctac gtccatccaa cctgaacgag ttgcaaaaag actgaggagt tcctcggcga
     5221 gcaggctatc gaattcaaac gtcgcgattt cttccgagag cagttcagcg ttgatgagct
     5281 tcgggagacg ctggcgtcgg tcggacttgg tccgttcgac gtcctctcga agcggtcgaa
     5341 ggcgtaccga aatcgacccg acgagatcga tgcgatgacc gacgacgaat tgctggcggc
     5401 gatggtcgcg gaaccgacgt tgctgcgccg tccactggtg attgccggcg ggcagcttgt
     5461 cgtcggtttc gaccgggcag ggttgaaggc actcaccggc cggcacgaca agagggagta
     5521 acaactgatg ttgaagcttg gtgcacatat gtcgatcgcg ggcgggttcg acaaagctgt
     5581 ccagcgcgca catagtgtcg aatcggacgc gctccagatc ttcaccaaga accagaagca
     5641 atggaaggcg aagccgatcc tcgacgagga tgtgacgttg ttccgccagc actgcctgga
     5701 ttgcgtgatc gatccggtgg ttgcccacga ctcgtacctg atcaacctgg cttcgccgga
     5761 cgatgagatg tgggagaagt cgatcgacgc gtttcggatc gagctggagc gctgcgagca
     5821 gatgggcatt ccgtttctgg taacgcatcc cggcgcgcac atgaagtcgg gcactgaggc
     5881 aggcgttgcc cgcgttgtcg ccgcgctgga tcgcattcac gtcgacctgc cgggctacaa
     5941 ggtcaagacg ctgctggaga caacggcagg tcagggcacg acgctgggcg cgacgttcga
     6001 ggagcttgcc gcgctgatcg ccggagtcgc cgcaccggag cgggtggcgg tctgctacga
     6061 cacgtgccac acatttgtgg ccggctacga tatccggaca ccggaggcgt acgccgagac
     6121 gatgtcgaag ttcgaccgca tcatcggcgt cgagcggatc gaggcgttcc atttcaacga
     6181 cgcgctgacc gagatcggct cgaagcgcga ccggcatgct catatcgggg ccggatatat
     6241 cggcgctgag ggcttccgca actttatgaa cgatccacgt ttcgccggaa aacccgccct
     6301 gctggagacg gagaagggcg aggacctggc cgaagatcgc gaggcgctca ttctgctgcg
     6361 ctcgctggtg gacggagcag ggggcggcag cgcggccgcc gcccctgctg tcgctacgcc
     6421 agccggttga cgaccagcaa gcgcgtctcg gtcatatcct cgatcgcgta gcgcaacccc
     6481 tcgcggctga gtccggagtc cttgactccg ccgtagggca tgtggtcgat ccggtagacc
     6541 ggcacgtcgt tgaggatgac gccgccgaca tccagccgct cgaacgcctg gagcgcgtgt
     6601 tcgaggttag cggtgaagac gccggcctgg aggccgaaga cggagtcgtt gatcttcgac
     6661 agcgtctcgt cccagtcctt tgtcgggaag acggtcacga ccggtgcgaa cgcctcgcgc
     6721 gagcagacga acgactcgcg ggcgacgtcg acgaggacgg ttggctcgta gaagcggccg
     6781 ttggcgtgcc cgccggtcag aacgcgtcca ccttccttga cggatgcctg gacccactcg
     6841 tcgatccggc gggccgcctt gtcatcgatc atcggtccca actcggtctc cgtgtcgagc
     6901 ggatctcccg tcttaatcgt cgcgacggcc gccgtcaact tctcgcagaa ctcgttgtag
     6961 acgtcctggt ggacgaagac gcgctggacg gagatgcaga cctgacccgc gtaggcgaac
     7021 gcgcccatcc ggacacgcga gacggcgaag tcgatgtcgg catcgcggtc gatcgcgacg
     7081 cctgcgttgc cgcccagttc caggacgacc ttcttcttgc cggcccgcga cttcatgtcc
     7141 cagccgacat cgggcgagcc ggtgaacgag agcagcttga agcgctcgtc ggtgacgagc
     7201 ctgtcgccga cagtgcgatc cattggcatg atcgagacgg cgcctttcgg cagcccaagc
     7261 tggtcgatca tctcggcgac ggtcagcatc gtcagcgggt cgagtgtggg cggcttgagg
     7321 acgatcgggt tgccggaggc gatagccggg gcgaccttgt gcagcgcgag gttgagcggg
     7381 aagttgaacg gtgagatgcc ggcgatcgg
//
LOCUS       DASUGJ010000273         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1846582, whole genome shotgun sequence.
ACCESSION   DASUGJ010000273 DASUGJ010000000
VERSION     DASUGJ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846582"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFV93_06095"
     CDS             <1..385
                     /locus_tag="VFV93_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5164948.1"
                     /translation="IDSSSITTFNEMRDNHLRTNDFFNAEQWPDMTFTSTRIEPKDDE
                     NFKIHGDLTIRDVTKPIVLDAEFEGLMEKDAMGKRRAAFTATTELNRREFGVNWNGTI
                     EAGGVVVGDKVKVTLNIALVQQDAA"
     gene            complement(408..1367)
                     /locus_tag="VFV93_06100"
     CDS             complement(408..1367)
                     /locus_tag="VFV93_06100"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5164949.1"
                     /translation="MASLSMRMITRDYYQMEPLANGTVTPEGIDLTIDRSVPMGVFLA
                     DERVPTGEMSFAQYVRRRAVGNCEVVGLPVFAMRGFRQRCFFARRGSGLQSFEDLAGR
                     RVGTNGWPDSGNTWSRSLVRRAGVELDQVSWSVGPIDDPAGKRPAHSDLPKGVRPLVG
                     GQTLVGMLTAGELDAIMIPWPPRAFYEPNSPIVRLLPDFRAAELDYARAMGFYPGLHI
                     VAVRSAYLAQQPWVAPSLFDAFEAAWRWAEDRLWSWTDSTPWLLDDLEQIRSIVGPDW
                     QAHGIEPNRAMIETFCNELAAQKLIERPVTVDELFGDFTRLAG"
     gene            complement(1501..4146)
                     /locus_tag="VFV93_06105"
     CDS             complement(1501..4146)
                     /locus_tag="VFV93_06105"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HEX5164950.1"
                     /translation="MNMQGFRLLNRRRGSLARVLARGHSRSLVPRQGLSAVMLLLAVV
                     LAGLGTAMPAQRVEAAAFPVTVKLTIEHIDAIGDLDGACGQADFYANVSVNGVSGTQG
                     PIDGNDHPDPNWAFSADATYDTGVSVPISIDLKEDDDTTCLGDDDIDINPGGGVGLSI
                     NVNLGSVPCTISGDTTGTCSTSITVQGNGGGDGDASLRFRVDVTNSQPDADGDGLSDD
                     WETNGVTFNGQFIDLPAMGADPNKADVFIQIDWMQNTSKDQRLSDNAIQRVVDAFANS
                     PYTNPNGSKGINLHVDQGKDSDLHRGSQPTTTWGDLSRAKAITFQNNLGTVDASGNYI
                     WTAFDAIKQANFVPTGRGPIFHYVIAAFLQEPPPSGGTQSTSSGISRNPDGTKFFDGA
                     SDFIISLGGTGGAGTEQQQAGTLMHEFGHNLGLGHGGNSATNYKPNYPSIMNYMFQMR
                     GLDVGADSGVLNYSSGTLPQLNEDNLDEDDGLGSPAAGMGTGHLCSLTTAATLDRRFI
                     ANANGPINWTCEKPAVIGDKVTFDANGNGMIDTGVTGLTDYNDWANIKFKGGQISALG
                     ESFLPSVTAAEPEVPLDTVPPTVTALETPPANANGWHNGSVTVTLSAVDNPGGEGVWD
                     ITYSATGAGALPQTTVAGDTASFVVSAEGITTVTFSARDRSGNVSTAATATIRIDVTK
                     PVVTLTCPLTVELNASGVTATWTASDALSGLVTPASGTVALDASAPGAHTATTPVAKD
                     AADNTNTASCTYYVIYKFTGFFAPVDNLPVLNSANGGQSIPVKWRITDANDVPIDAST
                     SFVNVSVVSLSCGSGTTSDQIEEYAAGNSGLQYQGDGYWQFNWKTPKSYRNSCKTMKL
                     NLDDTIGMTDAQLASIGRTVEFQFR"
BASE COUNT          768 a         1432 c         1354 g          842 t
ORIGIN      
        1 gatcgactcc tcttccatca cgacgttcaa tgagatgcgc gacaaccacc tgcgcaccaa
       61 cgacttcttc aacgccgagc agtggccgga catgaccttc accagcacgc ggatcgagcc
      121 gaaggacgac gagaacttca agattcacgg cgacctgacc attcgtgatg tcaccaagcc
      181 gatcgttctc gacgccgaat tcgagggcct gatggagaag gatgcgatgg gcaagcgccg
      241 ggccgccttc actgccacga ccgagctcaa ccgccgcgag ttcggcgtga actggaacgg
      301 cacgatcgaa gccggcggcg tcgttgtcgg cgacaaggtc aaagtcaccc tcaacatcgc
      361 ccttgtccag caggacgcgg cctgagtagc cgtcccgccc gcggacgcta tcccgccaga
      421 cgggtgaagt cgccgaagag ctcatctacc gtcactggcc gctcgatcaa cttctgggcg
      481 gccagttcgt tacagaacgt ctcgatcatc gcgcggttcg gctcgatgcc gtgcgcctgc
      541 cagtccggtc caacgatcga gcggatctgc tccagatcat ccagtagcca cggcgtcgag
      601 tcggtccacg accagagccg gtcctcggcc cagcgccagg ctgcctcgaa cgcatcgaac
      661 aggctcggtg cgacccacgg ctgctgcgcc aggtacgccg atcgaacggc aacgatgtgc
      721 agtcccggat agaagcccat cgcacgcgcg tagtccagct ccgccgcccg gaaatccggc
      781 agtagccgca cgatcgggct gttcggttcg tagaaagccc tcggcggcca cgggatcata
      841 atcgcgtcca actcgccggc cgttagcatc ccaaccagtg tctgcccgcc cacaagcggc
      901 ctgacaccct tcggcaggtc ggaatgcgcc ggccgctttc cggcgggatc atcgattggc
      961 ccaaccgacc aactgacctg gtccagctcc accccggccc ggcggaccag cgaccggctc
     1021 caggtgttgc ccgaatccgg ccagccgttg gtgccgacac gccgccctgc cagatcttca
     1081 aaggactgta gccccgaacc tcgccgcgcg aagaagcagc gttggcggaa tccacgcatc
     1141 gcgaagaccg gcagtccaac cacctcgcaa ttgcccaccg cccgccgccg cacatactgc
     1201 gcgaacgaca tctctccggt cgggacccgc tcatcggcca gaaacacacc catcggcacc
     1261 gagcgatcga tcgtcaggtc gatcccctcg ggcgtcacag tgccgttcgc cagcggctcc
     1321 atctgatagt aatcacgggt gatcatccgc atcgagagac tcgccactcg tcgcctccgc
     1381 ttccctccat gtcatcacgc cgggcaaccg gcgctcaaca cagtgtagcg ccgggtggcc
     1441 cgatgccacc cggcgctcca tcacggacgc ttgacagtag gtgcggctac gagctcgcag
     1501 ttatcggaat tggaactcca ccgtccgacc gatcgatgcc agttgggcgt ctgtcattcc
     1561 gatcgtgtca tcgaggttga gcttcatcgt cttgcacgag ttgcggtagc tcttcggcgt
     1621 cttccagttg aattgccagt agccatcgcc ctggtattgc aagcccgaat tacccgccgc
     1681 gtactcctcg atctggtcgg acgtcgtacc cgaaccgcac gagagtgaga cgacagacac
     1741 gttcacgaaa ctggtcgacg cgtcgatcgg aacgtcgttc gcgtcggtga tccgccactt
     1801 cactgggata ctctggccgc cgtttgccga attgagtacc ggcaggttgt cgaccggcgc
     1861 gaagaagccc gtgaacttgt agatcacata gtaggtgcag ctcgcggtgt tggtgttgtc
     1921 ggccgcgtcc ttcgcgaccg gtgtcgttgc cgtgtgtgcg cccggagcac ttgcgtcgag
     1981 cgcgaccgtc ccgctggccg gtgtcaccag ccccgagagt gcatctgatg cagtccacgt
     2041 tgcggtaacg ccggacgcat tgagctcgac ggttaacgga cacgtgagcg tgacaacggg
     2101 cttggtcaca tcgatgcgga tcgtcgctgt cgccgccgtg ctgacgttac cggaccggtc
     2161 tcgcgcgctg aaggtgacgg tcgtgatgcc ctcggcgctg acaacgaagc tggccgtatc
     2221 gcctgcaacg gtcgtctgcg gcagcgctcc tgcgccggtt gcgctgtacg tgatgtccca
     2281 gacgccttcg ccgccgggat tgtcgactgc actcagcgtg acggtcacgg acccgttgtg
     2341 ccagccgttc gcgttggcag gcggagtttc caaggcagtg actgtcggcg gaaccgtgtc
     2401 cagcggaacc tccggctcag cggctgtcac cgacggtagg aacgactcgc ccagcgcgct
     2461 gatctgccca cccttgaact tgatgttggc ccagtcgttg tagtcggtca ggccggtcac
     2521 accggtatcg atcattccat tgccgttggc gtcgaaggtg accttgtcac cgataaccgc
     2581 cggcttctcg caggtccagt tgatcggacc gttggcgttg gcgatgaagc ggcgatcgag
     2641 tgtcgcagcc gtcgtcaggc tgcagaggtg gccggtgccc atccctgctg cgggcgatcc
     2701 taggccatcg tcctcgtcga gattgtcctc gttcagctgt ggcagcgtcc cactcgagta
     2761 attgagcaca ccgctgtcgg cgccgacatc gaggccacgc atctggaaca tgtagttcat
     2821 gatgctgggg taattcggct tgtagttggt ggcggagttc ccaccgtggc ccaggccgag
     2881 attgtgccca aactcgtgca tcagcgtgcc ggcctgctgc tgctcggtgc cggcgccgcc
     2941 ggtaccgccg aggctgatga tgaagtcact ggcgccatcg aagaacttgg ttccgtcggg
     3001 gttgcgcgag atgccactgg acgtgctctg cgttccaccc gatggcggtg gttcctgcag
     3061 gaaggcggcg atcacgtagt ggaagatcgg gccgcggcca gttggaacga agttcgcctg
     3121 cttgatggcg tcgaaggcgg tccagatgta gttgccggaa gcgtcgaccg tcccgagatt
     3181 gttctggaac gtgatcgcct tcgcccggct caggtcaccc caggtggtcg tcggctggct
     3241 gccacgatgg agatcgctgt ccttgccctg gtcgacgtgc aggttgatcc ccttcgagcc
     3301 gttcggattg gtgtacggcg aattggcgaa ggcatccacc acccgttgga ttgcgttgtc
     3361 gctcaggcgc tggtccttgc tcgtgttctg catccagtcg atctggatga agacatccgc
     3421 cttgttcggg tcggcgccca tcgccggcag gtcgatgaac tgcccgttga acgtgacccc
     3481 attggtttcc cagtcgtcgg acagaccgtc accatcggcg tccggctggg aattggtcac
     3541 gtcaacgcgg aacctcaggc tcgcgtcgcc gtcgccgcca ccgttgccct ggacggtgat
     3601 cgacgtgctg caggtgccgg tcgtgtcgcc actgatggtg cacggtacgc tgcccaggtt
     3661 gacgttgatc gagagcccaa cgccgccacc ggggttgatg tcgatgtcat cgtcgccgag
     3721 gcaggtcgtg tcgtcgtctt ccttcaggtc gatcgagatc gggacgctca ccccggtatc
     3781 gtaggtggcg tcggcactga acgcccagtt gggatcgggg tggtcgttgc cgtcgatcgg
     3841 accctgtgtc ccggagacac cgttcaccga tacattggcg tagaaatcgg cctgcccgca
     3901 agcgccatcg aggtcaccaa tcgcgtcgat gtgctcgatc gtcagcttca cggtgaccgg
     3961 gaaggccgcg gcttcaaccc gctgcgccgg catcgccgtg cccagaccgg ccagcactac
     4021 cgccagcagc aacatcacgg cggacaagcc ctgccgtggt acgagagaac gggaatggcc
     4081 acgggcgagt accctcgcca gactgccacg ccgtcgattg aggagccgga atccctgcat
     4141 gttcatcgtc cctctcattg acgccgattg atccaccgca ttgttgatag agattgatta
     4201 ccttccggtc gctctccctt ccccaacgac gacggcccca gcacacgcgt gcggcgtgca
     4261 gaggtcgcgc tcgtcgcgtt cgtcgtgcct ggccgtgggg ccaggcacat tcgatatcga
     4321 acgaggcgtg atgtgattgt aggagggaga tatttgcaga acctttgcag cgcttgagcg
     4381 cgcgacgaag acgatc
//
LOCUS       DASUGJ010000274         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1429218, whole genome shotgun sequence.
ACCESSION   DASUGJ010000274 DASUGJ010000000
VERSION     DASUGJ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429218"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1145)
                     /locus_tag="VFV93_06110"
     CDS             complement(204..1145)
                     /locus_tag="VFV93_06110"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX5164951.1"
                     /translation="MDHINVQLLGRFAIRRNGHSVPHTDGLKLQELFSYLVLNRARSH
                     NRETLAGVLWGGGRSDQARKYLRQTLWQLQSSLAGEAPGEQHELLVVDSGWVHLNLQA
                     DISVDVMQLEQAFDLVRDVPGQAFTAEIAGAIMAAAGLYQGDLLEGWYQDWCLIERER
                     LQVIYLILLDKLMGYCEQQQEFERGIAYGHQILLIDRAREQTHRRLMRLHALAGDRTS
                     AIRQFERCQAMLAEDLDIDPSQRTVDLFARIRADRFPVAGTAASGEDQQLSLVDVSTH
                     LKQIRATLAGIDQQLQHDIQVVDQTMLDRADPAALDA"
     gene            complement(1375..2052)
                     /locus_tag="VFV93_06115"
     CDS             complement(1375..2052)
                     /locus_tag="VFV93_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5164952.1"
                     /translation="MTNSERAIHPNLAAERLGISVDCVYERIRRGELAARKEGGRWLV
                     LLDAATDVPAIELPPSEHPVVTAPSEPPAVVTAQPTESQSARAEELDEIRAQLAALEA
                     EIRALSSVVQRTQLGPVPVVTSPRELRPARLAFVRQRPRIVLLLGIIALVLGGSLIQA
                     SSAQRADDLTVAFVFACGAVTGAIVAWLALDKREPATPRNRRDPGKVRRPLVRKAGDG
                     DMRRRVP"
     gene            2205..2423
                     /locus_tag="VFV93_06120"
     CDS             2205..2423
                     /locus_tag="VFV93_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164953.1"
                     /translation="MNRDIVLDQHLELRARVAICRVYARAIENHLLNTDGDAQATEFA
                     ARIADHQAAIERLAGSLICQPEPVELPV"
     gene            complement(3115..>3647)
                     /locus_tag="VFV93_06125"
     CDS             complement(3115..>3647)
                     /locus_tag="VFV93_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5164954.1"
                     /translation="RGFNAGADDYVTKPADLLELLARANNLVRRFQQLDEQNTASVRV
                     GNAELSLGTLTYFSDDVGAQLLTPTEMRLIEYLMRRAGQTVSRADLIQHVWGIDVGDD
                     TNRIDVYVRRLRHKIERDPVSPRYLRTVRGAGYVFQDPALAGTSGEANVTPPVYRLDP
                     LDLTDDGEPRKPPSGI"
BASE COUNT          632 a         1204 c         1189 g          622 t
ORIGIN      
        1 cggtccgttg gtcctcggtc aagcgcacgc gatgggtgat ggacacggtg atctcctttc
       61 atcctgaccg cgatgctagc cgaccggtga caggacggca aattcacgct ggtcgactac
      121 tgagccgcag cgaaggacct ctccgctttg accacatagt cgttgagcga ggagaggtct
      181 tcgctgcggc tcaggacgac aggtcaggcg tccagcgccg ccggatcggc ccggtcgagc
      241 atcgtctggt caacgacctg gatgtcgtgt tgcagttgct ggtcgatacc ggccagcgtt
      301 gcccggatct gcttgaggtg cgtcgagacg tcgaccagcg agagctgctg atcctcgccg
      361 gaggctgccg tgccggctac cgggaaacgg tcggcccgga tccgcgcgaa cagatcgacc
      421 gttcgctggg atgggtcgat gtccagatcc tcggccagca tcgcctggca gcgctcgaac
      481 tgacggatcg ccgaggtgcg atcgccggcc agcgcatgca gccgcatcaa ccgccggtgc
      541 gtctgttccc gagcccggtc gatcagcagg atctggtggc cgtaggcgat cccgcgctcg
      601 aactcctgct gctgctcgca atagcccatc agcttgtcga gcaggatcag gtagatcacc
      661 tgcaaccgct cgcgctcgat caggcaccag tcctggtacc agccctccag cagatcgccc
      721 tgatagaggc cggctgccgc catgatcgcg ccggcaatct cggccgtgaa cgcctgaccc
      781 ggcacatcgc gaaccagatc gaacgcctgc tccaactgca tgacatcaac cgagatgtcg
      841 gcctggaggt tcagatgcac ccagccggaa tcgacgacca gcagctcgtg ctgctcgccc
      901 ggcgcttctc ccgccagcga cgattgcagt tgccagagcg tctggcgcag atacttgcgc
      961 gcctggtcag accggccgcc gccccagagg acgccggcca gcgtctcacg gttgtgcgag
     1021 cgcgcgcggt tgagcaccag atagctgaac agttcctgaa gcttgagccc gtcagtgtgg
     1081 gggacggaat gtccgttcct gcgaatcgca aaccggccga gaagctggac gttgatgtga
     1141 tccacgagcg tggccccttt cccgattcac cagatcggtc aggttcaccc ggcgcgatgg
     1201 cgcccgccgc cggtgtcgtg cctgcccatg cgcagggcga aacgacgcca cggcagcgag
     1261 aggatatgat tcgtttatgc ctccatagta gtcgagaagt gcctgtgtgt cactacatca
     1321 gcaaatattg cggggcatcg ttacgcgctc cgcaaatccg gcgcggggga ctcgctacgg
     1381 cacacggcgc cgcatatcgc cgtcgccagc cttccgaacg agtggccggc gtaccttgcc
     1441 tgggtcgcgc cggttgcggg gcgtggccgg ttcgcgtttg tcgagcgcca gccaggcaac
     1501 gatcgccccg gtcacggccc cgcaggcgaa cacgaacgcg accgtgaggt catccgctcg
     1561 ctgggcagag ctggcctgga tcaggctgcc gcccagcacc agcgcgatga tccccagcag
     1621 cagcacgatt cgcggtcgtt ggcggacgaa ggccaggcgc gccggacgga gctcgcgtgg
     1681 cgatgttacc accggaacag ggccgagctg cgtgcgctgg acgaccgacg acaacgctcg
     1741 aatctccgct tccagcgcgg cgagctgggc gcgaatctcg tcaagctctt cagcacgggc
     1801 actctgcgat tcggttggtt gagcggtgac gacagccggc ggctcactgg gagcggtcac
     1861 aaccggatgt tccgatggag ggagttcgat ggcaggaacg tccgtcgcgg catccagcag
     1921 cacgagccag cgtccgcctt ccttgcgggc agccagctcc ccgcgccgga tgcgctcgta
     1981 gacgcagtca accgaaatcc ccagccgctc ggccgcgaga ttggggtgga ttgcgcgctc
     2041 gctgttcgtc attggccgta tcctgacctg ccgccgctgt cgccatgaac tatagcggct
     2101 ccagcgccgt ttgccgtggc acgcaggatg ctgatagcgg gtctgtactc aacgcgcaac
     2161 aggggccacg atccgctcct acccgatggc agcagggagc ttcgatgaac agggacatcg
     2221 tactcgacca gcacctcgag cttcgcgccc gcgtagcgat ctgccgggta tacgcgcgag
     2281 caatcgagaa tcacttgctg aacacggacg gggatgccca ggcaaccgag tttgccgcgc
     2341 gcatcgccga tcaccaggca gcaatcgagc gcctcgctgg ctccctgatt tgccagccgg
     2401 agccggtgga gttgccggtc taggccagtt gcgcagcacc catcacgcga tcccacgcgc
     2461 aggggcgaga tatcggctcg ccctcctccc gggccggttt cggcgcgagc atgtacaatg
     2521 cgccccacga ccgatcggga tacatgagtg ggacgaccgc aagcatcgtg acgcatgtga
     2581 cgctgatatg gaaacgcctc atgcgtcggc cggaattgct cgccggtgtc gtgctggccg
     2641 ttgactggat ccttgccagg cacaagccgg gcctgctgat cgccggcacg ctcgatgaaa
     2701 cggcccattt cgcgacgacg atgctgatcg gcgggccgtt cccagaacgc gacgatatat
     2761 ccgttgtcgc cggggccctg atcggctcgg tcctgatcga cgcggaccac gtcccgctgg
     2821 tcctcgccaa acatccgctc gaatcggccc aggaccggcc cgcgacacac tcgctgttga
     2881 cgcctgttgc gctcggcggg ctgggccatg tcacaaacgg gacacctcga aagctcctgc
     2941 tggggatcac ggccgggaca acctgccact tcctgcgcga cctggcgact ggcgggatac
     3001 cgctcatctg gccagccagc cggcggctgg tgtcggttcc ctatgctgcc tacgcggggg
     3061 tgctggccgc gctggttgtg cgggcgaggt tcgcgaacgg cgctagcgcc gggctcagat
     3121 gccggacggc ggcttgcgtg gctcaccgtc gtctgtcaga tcgagcggat cgaggcgata
     3181 gaccggcggc gtcacgttgg cttcgccact cgtgccggcc agcgccggat cctgaaagac
     3241 ataccccgcg ccgcgaaccg tcctgagata gcgcggcgag acagggtcgc gctcgatctt
     3301 gtgccgcaag cgacggacgt aaacatcaat ccggttcgtg tcgtcgccaa cgtcgatgcc
     3361 ccagacgtgc tgaatcaggt cagcccgaga gaccgtctgg ccagcgcggc gcatcagata
     3421 ctcgatcagg cgcatctcgg tcggggtcag cagctgcgcg ccgacgtcgt cactgaagta
     3481 ggtcagggtg cccagcgaca actccgcgtt gccgacacgg acgctggccg tgttctgttc
     3541 gtcaagctgc tgaaaccgtc ggacgaggtt attcgcgcgg gccaggagct ccagcagatc
     3601 ggccggcttc gtcacatagt cgtcggcgcc ggcgttgaag ccacgga
//
LOCUS       DASUGJ010000275         3348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1014459, whole genome shotgun sequence.
ACCESSION   DASUGJ010000275 DASUGJ010000000
VERSION     DASUGJ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014459"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFV93_06130"
     CDS             <1..239
                     /locus_tag="VFV93_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164955.1"
                     /translation="APLFAAFERSRLVEECGPIGELMIEEFRTNRIQVHAFVVWRNRH
                     HPGEPFSDELLRAFQQADRWEYTEPAYHVNRDVS"
     gene            complement(220..534)
                     /locus_tag="VFV93_06135"
     CDS             complement(220..534)
                     /locus_tag="VFV93_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164956.1"
                     /translation="MTFAYRELWTLIITFGPVAGFIAVGPEVGAGVLAVAEQFLAGAG
                     GDDERTADSQLRTWLDGVAAGQRDAVQATVAALDWTGLRWVGFRRHGAPASCCGVNSR
                     HD"
     gene            575..886
                     /locus_tag="VFV93_06140"
     CDS             575..886
                     /locus_tag="VFV93_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5164957.1"
                     /translation="MNLDEYIAKRLADDPEFRAAYEASRPEFEFRRQLIRARLNARLT
                     LEQLAERIGTKRSSISRLEGRDAEPSFDMLRQLSAALNVSFEILPTSAIVIHEFDPVH
                     A"
     gene            complement(943..1335)
                     /locus_tag="VFV93_06145"
     CDS             complement(943..1335)
                     /locus_tag="VFV93_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164958.1"
                     /translation="MPTSPEQVDIEDDLYRRIVPDNVTNGELNAGAFSLRPGEDALSV
                     DIARLTSVEECLARAGRPDVGLSVLSVGDVVALGLTVEHDPVVGDAASGIPANYAHAR
                     ITGENSRAIRSKLAKAAVILVSPERRPS"
     gene            complement(1335..1790)
                     /locus_tag="VFV93_06150"
     CDS             complement(1335..1790)
                     /locus_tag="VFV93_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164959.1"
                     /translation="MVHEQHSDGEAMASNSIVNAAPSVELSQDIERVTSLTRLEDDWD
                     TYGGAPVTSSAAASVTNVLRSFAEAHDGSNYTAVRPHSITPLASGGVNVEWHGPFGEL
                     TLEVYPDGRLGCYIVESRDGQTTERDEEEPALQTVMTSLRRILGVQQRA"
     gene            1921..>3348
                     /locus_tag="VFV93_06155"
     CDS             1921..>3348
                     /locus_tag="VFV93_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX5164960.1"
                     /translation="MMQRVSRRTFLKVGGATAAVVASGALVGSMLTDDNDDPDPVDPF
                     NPFNPADPQAHVTPTVGPRIDEEVGGYLAIAPRVLRAGQLQTVSLALFAGQRIASSTV
                     SVELIKGDATIASGSAWVAGRGTVPLQLPDIAAGDYRLKVSTRGFEEMADVQIEDGTL
                     VFVETDKPIYKPGQTVHIRVLTLDPALRPVTGDVSVEVLDAQGIKIFRRTVSSDEYGM
                     AALDLPLSTEPNLGVWTVLAETGQRSTQVDIRVERYVLPKYEVRIELERDWSLVNEPI
                     SGLIAAEYAFGKPVNGEVEIVASRYVGAWEQYATVRRPISDGRLVFEIPAVEYVTGSP
                     DGGGLGSVQLEAKVIEQATGYEETTTQLVSIAQGPVVLQIIPDSSSFKPGLPLTLLVV
                     TETPGRQPVDARVYLNLSWQDDGYGDAGFDNETVDTSNGVATLTITPPNDAAILTMDA
                     SVDEMTSTYLTLRAGYSPSNTFIHVT"
BASE COUNT          623 a         1099 c         1039 g          587 t
ORIGIN      
        1 acgcgcctct cttcgcggcc ttcgagcgct cgcggctggt cgaggagtgc ggcccgatcg
       61 gcgagctgat gatcgaggag ttccgtacca accgcatcca ggtccacgcc ttcgtcgtct
      121 ggcgcaaccg ccaccatccc ggtgagccgt tctccgacga gctgctgcgc gcgttccagc
      181 aggccgaccg ttgggagtac actgaacccg cgtatcacgt caatcgtgac gtgagttgac
      241 gccgcagcag gaagcaggcg ccccgtggcg acggaatccg acccatcgca gcccggtcca
      301 gtcgagcgcg gctactgtcg cctgcaccgc gtcgcgctgc ccagccgcta ccccgtccag
      361 ccacgtccgg agctggctat ccgccgtccg ctcgtcgtcg ccacccgccc cggccaggaa
      421 ctgctctgca acggcgagca ccccggcccc cacctctggc ccgacggcga tgaatccggc
      481 gactggcccg aaggtgatga tcaacgtcca caactcgcga taggcgaacg tcatggaacg
      541 gtgctagaag tcaacggaca gaggggtccg gcatatgaat ctggatgaat acatcgccaa
      601 gcgcctcgcc gatgatccag aatttcgcgc cgcatatgag gcatcacgac ctgaattcga
      661 gttccgtcgc cagttgatcc gcgcacggtt gaacgcccgc ctgacactag agcaactcgc
      721 ggaacggatc gggacgaaac gatcgtcaat ctcgcggctt gaaggaaggg acgccgagcc
      781 gagcttcgac atgttgcgcc agctgtctgc cgccctcaac gttagctttg aaattctgcc
      841 gacgtccgcg atcgtgatcc acgaattcga tcctgtgcac gcttgacact ggcatgccgg
      901 tggtcgcgca cgctggttga tcatgtcagg ttgctctcgc ggtcagcttg ggcgtcgttc
      961 tggactgacg agaatcactg ccgctttggc cagtttcgaa cgaatcgcgc gcgagttctc
     1021 gccggtaatc cgtgcgtgtg cgtaattagc ggggatgccc gaggccgcat ctccaacaac
     1081 cggatcatgt tctacggtca gtccaagcgc gaccacatcg cccacactga ggacggacag
     1141 tccaacatcc ggacggcccg ctcgggcgag gcactcctca accgatgtca ggcgagcgat
     1201 atcaaccgag agcgcgtcct cgccggggcg gaggctgaat gcaccagcgt tgagttctcc
     1261 attggtgacg ttgtcgggaa cgatacgtcg atacaggtcg tcttcgatat cgacctgttc
     1321 aggcgatgtt ggcatcaggc gcgttgctga acaccgagga tacgtcgcag cgaggtcatc
     1381 accgtctgca acgcgggttc ctcctcgtca cgctcggtgg tctggccatc gcgtgactcg
     1441 acgatgtagc aacccaggcg accatccgga tagacctcaa gcgtcaactc accgaacggg
     1501 ccgtgccatt cgacgttgac gcctccggat gcgagcggcg tgatgctgtg aggccgaacg
     1561 gcggtgtagt tcgacccatc atgtgcttcg gcaaatgatc gcaacacgtt cgtgacgctg
     1621 gctgcggcac tcgacgttac cggcgcgccg ccgtaggtgt cccagtcgtc ttcgagcctc
     1681 gtcaacgatg tgacccgctc gatgtcctga ctcagttcga cagacggggc cgcattcacg
     1741 atcgagttgg atgccatcgc ttcgccgtcc gaatgctgtt cgtgaaccaa cgtgacaaac
     1801 ctcccatgat aactccatca tacgccagga catcccaggc gggtagccca acggacatca
     1861 ccgggaactt tccgcggccg gccggcgtat tccctccaac gcacagaatg gaggccggca
     1921 atgatgcagc gtgtgtcgag gcggacgttt ctcaaggtag gcggcgcgac ggcggcggtg
     1981 gtcgcttccg gcgcgctggt cgggtcgatg ttgaccgatg acaacgatga tcctgatcca
     2041 gttgacccct tcaatccatt caatccagcc gacccgcagg cgcacgtcac gccgacggtc
     2101 ggcccacgca tcgacgagga ggtcgggggc tacctggcga tcgcgccgcg tgtcctgcgg
     2161 gccggtcaac ttcaaaccgt ctcgctggcg ctgtttgccg ggcaacggat cgccagcagc
     2221 accgtctcgg tcgaactcat caaaggtgac gcgaccatcg ccagcggctc ggcctgggtg
     2281 gcagggcgcg ggacggtccc gctccagctc cccgacatcg cggccggcga ttaccggctg
     2341 aaggtctcga cgcggggctt tgaagagatg gctgacgtcc agatcgagga cggcacactc
     2401 gtcttcgttg agacggacaa gccgatctac aagccggggc agaccgtcca catccgcgtc
     2461 ctgacgctcg atccggcgct ccgacccgtc accggcgacg tctcagtcga ggtgctggac
     2521 gcccagggaa tcaagatctt ccgccgcacc gtcagcagcg acgagtacgg gatggcagcg
     2581 ctcgacctgc cgctctcgac agagccgaac ctcggcgtct ggacggtact ggccgagacc
     2641 ggccagcgca gcacccaagt tgatatccgc gtcgagcgct atgtcctgcc gaagtacgag
     2701 gtgcggatcg agctggagcg cgactggtcg ctcgtcaacg agccgatcag cggcctgatc
     2761 gctgccgagt acgccttcgg caagccggta aacggtgagg ttgagatcgt cgcctcgcgc
     2821 tacgtcggcg cctgggagca gtacgcgacc gttcgccggc cgatcagcga tggccggctc
     2881 gtcttcgaga tccccgctgt cgagtacgtg accggctcgc cggacggcgg cggcctcggc
     2941 agcgtccagc ttgaggcgaa agtcatcgag caggcgaccg gctacgagga gacgacgacg
     3001 cagctcgtgt cgatcgcgca ggggccggtc gtcctgcaga tcattcccga cagcagcagc
     3061 ttcaagccgg ggttgccgct gacgctgctg gtcgttaccg agacgccagg caggcagccg
     3121 gtcgatgcgc gcgtctacct caatctcagt tggcaggacg acggatacgg cgacgccggc
     3181 ttcgacaacg agaccgtcga taccagcaat ggcgtcgcga cgttaaccat cacgccgccg
     3241 aacgacgcgg cgatcctgac gatggatgcc tcggtcgatg aaatgacctc tacctatctc
     3301 acgctgcggg cgggctactc gcccagcaac acgttcatcc atgtgacg
//
LOCUS       DASUGJ010000276         7854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1291694, whole genome shotgun sequence.
ACCESSION   DASUGJ010000276 DASUGJ010000000
VERSION     DASUGJ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291694"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VFV93_06160"
     CDS             <1..1086
                     /locus_tag="VFV93_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX5164961.1"
                     /translation="SSIYTLGQDARAGLDWARGTLAGLLGCQPREITMTSGATESNNL
                     AVKGVAWWHRFNDPARKNHIVVSAIEHHAVLHAAEALIPHGFEVSIVQPDTDGIVRPE
                     AVEAALGPDTCLVSVMYANNEIGTIQPIHKIAALAHERGIPVHTDAVQAAGALPLDVD
                     DLGVDLLSLSAHKFYGPKGVGLLYVRRRTPIEWQQQGGGQEGGHRGGTENVPLIVGMA
                     AALALATDELDERAARMRDLRDQLIEGILERVPDARLNGHPTHRLPNNVNFSFAGVEG
                     ETLLLNLDMLGIAASSGSACSTGSTEPSHVLTALGLGPEIARGSLRLTLGKDTTEAEI
                     ERTVAVVAETVARVRTMGEAPIEAGRV"
     gene            complement(1087..1644)
                     /gene="scpB"
                     /locus_tag="VFV93_06165"
     CDS             complement(1087..1644)
                     /gene="scpB"
                     /locus_tag="VFV93_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5164962.1"
                     /translation="MSHSQHDEQAMLEISVESAEDAAAAIEALLFASGSAEEPSTLAA
                     ALGWSQADVRRGVDELERQLRENRRGMVLQRDGSTVQLVSAPRFGKPIARLLGMERSA
                     KLSSAALETLALVAYRQPVTRSEIEAVRGVDSSGVLATLVARELIDPRGRRSTPGNPV
                     EYGTTAAFLQFFGLTSLDELPPLSD"
     gene            complement(1641..2375)
                     /locus_tag="VFV93_06170"
     CDS             complement(1641..2375)
                     /locus_tag="VFV93_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX5164963.1"
                     /translation="MPTLANYQLRLPSFEGPLDVLLGLIERERLEISDLSLVTVTDGF
                     LGYIESLDDAPPALLAEFAGVAARLLVLKSRALLPRPETVEVEPDVDDLAQQLREYQR
                     AKQVAESFRALEQAGRRTFVRPPMSDLPAPRIVLVPPPVGHLRRALVRTLARVRAEPE
                     VTALRKVVTIGEMLDRLRHRIARVRGRARFHEMIGSNARDDTVVGFIALLALWRRGEV
                     HVEQDGLFEDIHVMPMSGAPVGGGDQ"
     gene            complement(2403..2969)
                     /locus_tag="VFV93_06175"
     CDS             complement(2403..2969)
                     /locus_tag="VFV93_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5164964.1"
                     /translation="MRMTIASSPLEPRRGWLHGAIWRVRQFGHAVRSRPDPAVDSELR
                     RLLASDAQWELLARLTPFDRAHHLRVYRLLADAGQDDPDLLRAALLHDSGKAAERGRV
                     GVVHRAVYVLVGRVSPALLDRLASHDDRFRHGIWLSLRHAEIGAELVRDAGGSERCCD
                     LIRRHNDRSASDDPLLAALIADDHAAIR"
     gene            complement(2969..3628)
                     /locus_tag="VFV93_06180"
     CDS             complement(2969..3628)
                     /locus_tag="VFV93_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5164965.1"
                     /translation="MFGSSLDSDVIFARIVTLIVAVTIHEFAHAWSSYQLGDPTAARL
                     GRITLNPAAHFDPIGFLGMMMIAIGWPAFGWGKPVPVNPNLVRGHKAGMAITAAAGPL
                     SNLIMATLVVIPIRFGGVEPTGFGAVLVEQFIFVNLLLAAFNMIPLPPLDGSKILAGF
                     LPDFWYPYIARLEQYAFAGLLVLIIINRQFADGGRDIIGTMYFPVFDFLRSTIVGSTL
                     F"
     gene            complement(3749..5254)
                     /locus_tag="VFV93_06185"
     CDS             complement(3749..5254)
                     /locus_tag="VFV93_06185"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX5164966.1"
                     /translation="MSTTTASTTSTNGKVQEEILLDVKNLVMHFPLTQGIIFQRKVGA
                     VQAVDDISFHVRRGETLGMVGESGCGKSTTGRAILQLYKPTSGEVVFNGRDLTKVDGG
                     EMRKMRRYLQMIFQDPYASLNPRMTVGSIISEPMQIHNLVPRDQRNRRVQELLETVGL
                     NPYFANRYPHEFSGGQRQRIGVARALAASPDFIVCDEPVSALDVSIQAQIVNLLEDLQ
                     EQFNLTYLFIAHDLSVVRHISDRIAVMYLGKLVELADRDALYEDPLHPYTKALLSAVP
                     IPDPVVEKRRERIILTGDVPSPINPPSGCHFHTRCPYVMPVCKEIDPIFVDQGGGHFV
                     ACHLYPGSGADGSTTINRTSTPVAAAPAAASAAASASTVTAEPPQAQTQAFVAAAAAV
                     ATTGSVRISKVLGDPKEEAGATHEYVELTNDDAADVQLDGWRVLDKDDNTYTFPAFVL
                     TSGSTVRLHIAEGEDSSSDLYWGMSGPMLNNKGDRIRLYDSTGAMVQAAES"
     gene            complement(5258..6253)
                     /locus_tag="VFV93_06190"
     CDS             complement(5258..6253)
                     /locus_tag="VFV93_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5164967.1"
                     /translation="MEPLLDVKNLKTQFFTQDGVVRAVDDVTFHIMPGETLGVVGESG
                     SGKSITAMSIMRLIPTPPGRIVNGSVNFEGDDILAMSDEEMRSVRGNKIAMIFQDPMT
                     SLNPVLNINRQIGETLELHLGMSKSQSRKRAIELLSMVGIPNAERRVDQYPHQFSGGM
                     RQRVMIAMALSCNPTLLIADEPTTALDVTIQAQILDLMRSLQVEHNTALMLITHDLGV
                     VAGMTDRIQVMYAGHIVETAPTEELFANPRHPYTVGLLNSIPRLDASAKTKLDPIRGM
                     PPDLIDLPDMCPFLPRCDYAREKCEQQNPPLLDVNAEHRSACWFWEEVSKEGPKV"
     gene            complement(6541..7479)
                     /locus_tag="VFV93_06195"
     CDS             complement(6541..7479)
                     /locus_tag="VFV93_06195"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX5164968.1"
                     /translation="MAEIPLQPYAYFEGQIIPSDEAKVSIATHALQYGTGAFGGIRGY
                     LDVSEESINIFRLPDHTRRLMNSSRILRSSLKLDADGLATVIQDLVAQNNPRSNIYIR
                     PFIYKAGLELTPRLNGIRDDIAIYIISFGDYFAMDKPLRVLVSSWVRIADSIIPSRGK
                     ITGGYINSSLAKDQAESAGFDDAIMLNISGKVAEGSGANLFIVRNGTLITSPITGDIL
                     EGITRRSVVRFARDAGIPVEEREIDRSELYVADEAFFCGTGAQVASIGDIDGRPVGNG
                     ERGEITKTIQDIFFACVRGQDAKYGHLLTKVPVRPT"
BASE COUNT         1439 a         2540 c         2518 g         1357 t
ORIGIN      
        1 tccagcatct acacactggg acaggacgcc cgcgccgggc tcgactgggc gcgcggcacg
       61 ctggccgggc tgctcggctg ccagccgcgc gagatcacga tgaccagcgg cgcgaccgag
      121 tcgaacaacc tcgccgtcaa gggtgtcgcc tggtggcacc gcttcaacga tccagcccgc
      181 aaaaaccaca tcgtcgtctc cgcaatcgag catcatgccg tcctgcatgc ggccgaggcg
      241 ttgatcccgc acggcttcga ggtcagcatc gttcagccgg acaccgacgg catcgtccgg
      301 cccgaagctg ttgaagcggc actcggtccc gacacctgtc tcgtctcggt catgtacgcc
      361 aacaacgaga tcggcacgat ccagcccatt cacaagatcg cggcgctggc ccacgagcgg
      421 ggcatccccg tccacacgga tgccgtccag gcggccggcg cgctcccgct cgacgttgac
      481 gatctgggcg tcgatctgct ctccctctcg gcccacaagt tttacggacc gaagggcgtc
      541 ggcctgctct atgtccgccg gcgaacgccg atcgagtggc agcagcaggg tggcggccag
      601 gagggcggcc atcgtggagg gaccgagaac gtgccgctga tcgtcgggat ggcggcggca
      661 ctcgcgctgg cgaccgacga actggatgag cgcgcggcac gtatgcgcga cctgcgtgac
      721 caactgatcg aaggcatcct cgaacgagtc cctgacgccc ggctcaacgg acatccgaca
      781 catcggctgc ccaacaacgt caatttctcg ttcgccggtg tcgaaggcga gacgttgctg
      841 ctgaacctgg acatgctcgg catcgccgcc agcagcggct cggcctgctc gaccggctca
      901 accgagccat cgcacgtcct gacggcactc ggcctcggcc ccgagatcgc gcgcggctcg
      961 ctgcggctga cgctcggcaa ggacacgact gaggccgaga tcgagcgaac ggtcgccgtc
     1021 gtcgctgaga ccgtcgcacg agttcggacg atgggtgaag cgccgatcga agcagggcgg
     1081 gtctgatcag tcgctgagcg gcggcaactc gtcgagcgac gtcaggccga agaactgcag
     1141 gaacgcggcg gtcgtaccgt attcaaccgg gttgccgggc gtcgagcgcc ggccacgtgg
     1201 atcgatcagc tcgcgggcaa ccagcgttgc cagcacacca ctcgaatcga ccccacggac
     1261 ggcctcgatc tcggagcggg taacaggttg gcggtaggcc accaacgcca gcgtctcgag
     1321 cgcggcgctt gagagcttgg cggagcgttc cattcccagc agccgggcga ttggcttgcc
     1381 gaagcgtggt gcgctgacaa gctggacggt ggagccgtcc cgttgcagta ccatcccgcg
     1441 ccggttctcg cgcagttgcc gctcaagctc atccacgccg cgccggacgt cggcctggct
     1501 ccatcccagc gcagccgcca gcgtcgacgg ctcctcggcg ctgcccgaag cgaacagcag
     1561 cgcctcaatc gcggcagcgg cgtcttcggc cgattcgaca ctgatctcca gcatcgcctg
     1621 ctcgtcgtgc tgggagtgac tcactggtca cccccaccga ccggggctcc gctcatcggc
     1681 atgacatgga tgtcctcaaa caggccatcc tgctcgacat gcacctcccc acgccgccag
     1741 agggccagca gcgcgatgaa gccgacaaca gtgtcgtcgc gggcgttcga gccgatcatc
     1801 tcgtggaagc gcgcccggcc gcggacccgc gcgatccgat gacgcaggcg atccagcatc
     1861 tcgccgatcg tgacaacctt gcgaagagcg gtgacctccg gctcggcccg gacgcgtgcc
     1921 agcgtgcgta ccagcgcacg tcgcaggtgt cccaccggcg gcggcaccag cacgatgcgc
     1981 ggggcgggca ggtcggacat gggtggccgg acgaacgttc gccggccagc ctgttccagt
     2041 gcacggaacg actcggcgac ctgcttggcg cgctggtatt cgcggagttg ctgggcgagg
     2101 tcgtcgacat ccggctcgac ctcaaccgtc tccggccgcg gcaggagcgc acgcgacttg
     2161 agcaccaaca agcgggcggc gacgccggca aactccgcca gcagggccgg cggcgcgtca
     2221 tcgagcgatt cgatgtagcc gaggaagccg tccgtgacgg tcaccagcga cagatcggag
     2281 atctccagcc gctcgcgttc gatcaggccg agcaggacat cgagcggccc ctcgaagctc
     2341 ggcaggcgca actggtaatt ggccaatgtc ggcacggcac cgttcgtcgt cacggctccc
     2401 tctcagcgga tagcggcatg gtcatcggcg atcaacgccg ccagcagcgg atcgtccgat
     2461 gccgagcgat cattgtgtcg ccggatcagg tcgcagcagc gttctgaacc accggcgtcc
     2521 cgcaccagtt ccgcgccgat ctcggcatgg cggagactca accatatccc atgccgaaac
     2581 cggtcatcgt gtgacgcaag tcgatcgagc agcgccggcg agacacgccc gaccaggacg
     2641 tagacggccc ggtgcaccac gccgacccga ccacgctcgg ctgccttacc ggagtcatgg
     2701 agcaacgctg cacgcagcag atcaggatca tcctggccgg catcagccag caaacgatac
     2761 acacgcaggt gatgggcgcg gtcgaatggc gtcaggcgtg ccagcaattc ccactgtgcg
     2821 tcggaggcca gcaagcggcg cagttcgctg tcaacggctg gatcggggcg actgcgaacc
     2881 gcgtgcccga actggcgcac ccgccaaatc gcaccgtgca gccagccccg cctcggttcc
     2941 agtgggctgg acgcgatcgt catgcgcatc agaacaacgt cgacccgacg atcgtcgagc
     3001 gcaggaagtc gaagaccgga aagtacatcg ttccgatgat gtcccgaccg ccgtcagcaa
     3061 actgtcggtt gatgatgatc agcaccagca gccctgcgaa cgcgtactgc tccaaccggg
     3121 cgatgtaggg gtaccagaag tcaggcagga agccggccag gatcttcgag ccatccagcg
     3181 gcggtagcgg gatcatgttg aaggccgcca gcagcaggtt gacgaagatg aactgctcga
     3241 ccagaacagc cccgaatccg gtcggctcga cccctccgaa gcgaatcggg atcaccacca
     3301 gcgttgccat gatcaggttc gacagcggcc cggcagcggc cgtaatcgcc attccggctt
     3361 tgtgaccacg gacgagattc gggttgaccg gcaccggctt gccccagccg aacgccggcc
     3421 agccgatggc gatcatcatc atgccgagaa agccgatcgg gtcgaagtgc gcggccgggt
     3481 tgagcgtgat ccgtcccaac cgggcggccg tcgggtcgcc gagctggtag gacgaccatg
     3541 cgtgcgcgaa ctcatggatg gtgacggcca cgatcagcgt gacgattctg gcgaagatga
     3601 catcgctgtc gagagaacta ccgaacatgc acgtcaatcg tctttcgggc gagaaccggt
     3661 tcagtcatct gagtaaacac taccagcacc gaaacgggga gggcgattgc cctccccgtg
     3721 atccgattcc ttgatagtcg ctcgacgatc aggactcagc agcctggacc atcgcgccgg
     3781 tgctgtcata cagccggatt cgatcgccct tgttgttcag catcgggccg gacatgcccc
     3841 agtagagatc gctggaggag tcctcgccct cggcgatgtg cagccggacg gtcgatcccg
     3901 acgtcaacac gaacgccggg aaggtgtagg tgttgtcgtc cttatcgagt acgcgccagc
     3961 cgtccagttg gacatcggca gcgtcgtcat tggtcagctc cacgtactcg tgggtggcgc
     4021 cagcctcttc cttcgggtcg cccaggacct tcgagatgcg cacgctacca gtcgtcgcaa
     4081 cggcagcggc ggctgccacg aacgcctggg tctgagcctg cggcggctcg gcggtgaccg
     4141 tcgatgcgga tgcagccgcc gaagcggcgg ccggagcggc ggccactggc gtcgacgtgc
     4201 ggttgatcgt cgtcgaaccg tcggcgccgg agcctgggta caggtgacag gcaacgaagt
     4261 gaccgccgcc ctgatccacg aagatcgggt cgatctcctt gcagaccggc atcacgtacg
     4321 ggcagcgcgt gtggaagtga cagccggacg gcgggttgat cgggctgggc acgtcgccgg
     4381 tgagaatgat gcgctcgcgc cgcttctcaa cgaccgggtc cggaatcggc acagccgaga
     4441 ggagcgcctt ggtgtacgga tggagcggat cttcgtagag cgcatcgcgg tccgcaagct
     4501 cgaccagctt gccgaggtac atcacggcga tccggtcaga gatgtggcgc acgaccgaca
     4561 ggtcgtgggc gatgaacaga tacgtcaggt tgaactgctc ctgaagatct tcgagcaagt
     4621 tgacgatctg cgcctgaatc gacacgtcca gcgccgagac cggctcgtcg cagacgatga
     4681 agtccgggct ggccgccagc gcacgggcaa caccgatgcg ttgccgctgg ccgccggaga
     4741 actcgtgcgg gtagcggttg gcgaagtacg ggttgagacc caccgtctcc agcagttcct
     4801 gtacgcgccg gttgcgctga tcgcgcggga ccaggttgtg gatctgcatc ggctcggaga
     4861 tgatcgagcc gaccgtcata cgcggattca gcgaggcgta tgggtcctgg aagatcatct
     4921 gcaggtagcg gcgcatcttg cgcatctcgc cgccatcaac cttggtcagg tcgcgcccgt
     4981 tgaagacgac ctcgccggag gtcggcttgt agagttggag gatcgcgcgg ccggtcgtgc
     5041 tcttgccgca accggactcg ccgaccatcc ccagcgtctc accgcgccga acgtggaacg
     5101 agatgtcgtc aacggcctgg accgcgccga ccttgcgctg gaagatgatg ccctgggtca
     5161 gcgggaagtg catgaccagg ttcttgacat ccagcaggat ctcttcctgg accttgccgt
     5221 tggtggacgt cgtgctcgca gtggttgttg acatgtgcta aaccttcggc ccttctttgc
     5281 tgacttcctc ccagaaccag catgccgatc ggtgttccgc gttcacgtcg agcaacggcg
     5341 ggttctgctg ctcacacttc tcgcgcgcgt agtcgcagcg cgggaggaac gggcacatgt
     5401 ccggcaggtc gatgaggtcg ggtggcatgc cacggattgg atcgagcttg gtcttggcgg
     5461 acgcgtcaag tcgagggatc gagttgagca gtccgacggt gtacgggtgg cgcgggttgg
     5521 cgaacagctc ttccgtcggc gccgtctcga cgatatgccc ggcgtacatg acctgaatgc
     5581 ggtcggtcat gccggccacg acaccgaggt cgtgcgtgat cagcatcagg gccgtgttgt
     5641 gctcgacctg gagactgcgc atcaggtcaa ggatctgggc ctgaatcgtc acgtcgagcg
     5701 ccgtcgtcgg ctcgtcggcg atcagcaacg tcgggttgca cgagagcgcc atcgcaatca
     5761 tcacgcgctg gcgcataccg ccggagaact ggtgcggata ctgatcaaca cgccgctcgg
     5821 catttggaat accgaccatg ctgagcagct cgatcgcacg cttgcgcgac tgcgacttgc
     5881 tcatccccag gtgcagctcg agcgtctcgc cgatctgccg gttgatgttg aggaccggat
     5941 tgagcgaggt catcgggtcc tggaagatca tcgcgatctt gttgccccgg acgctgcgca
     6001 tctcctcgtc gctcatcgca aggatatcgt cgccctcgaa gttgaccgaa ccgttgacga
     6061 tgcggcccgg cggcgtcggg atcaacctca tgatcgacat ggcggtgatg ctcttgccgg
     6121 atcctgattc gcccacgaca ccgagcgttt cgccgggcat gatgtggaac gtcacatcat
     6181 caaccgcccg gacgacccca tcttgtgtga agaactgagt cttcagattc ttcacgtcaa
     6241 gtagcggttc catccctgct ccttcgcctc ccgcatcaat acccccggga tcgttatcgt
     6301 gccccacaac accgatccgg aatccccaaa acgctcacgc ggcaccgccc cctgttacgg
     6361 tatgcgcgtg tccggcccac gggccggcgt tgattgttca ttcaatctac gtctgggctg
     6421 ccgaatctgg tctggcgtgg cgtcgaatcg agtatagcac ggtccaaaaa atctggtcaa
     6481 acgtatcgtt tccatgctcc gtgaacgatc acggagcagg atactatcag atttcgacgc
     6541 ctaggttggc cgaaccggca ctttcgtcag cagatgcccg tacttggcgt cctgaccgcg
     6601 cacgcaggcg aagaagatgt cctggatcgt cttcgtgatc tccccgcgct cgccattccc
     6661 gaccggccgg ccgtcgatgt cgccgatcga tgccacctga gcgccggtcc cgcagaagaa
     6721 cgcctcatca gcgacataca gctccgagcg gtcgatctca cgttcctcga ccggaatgcc
     6781 ggcgtcccgc gcgaagcgga cgaccgaacg gcgggtgatt ccttcgagga tgtcgccggt
     6841 gatcgggctg gtgatcagcg tcccgttacg gacgatgaac aggttcgcgc cgctgccctc
     6901 ggccaccttg ccggagatgt tcagcataat cgcgtcgtcg aagccggccg attccgcctg
     6961 atctttcgcc aggctggagt tgatgtagcc gccggtgatc ttgccccggc tcgggatgat
     7021 cgagtcggcg atacgcaccc aggacgagac cagcacacgc agcggcttgt ccatcgcgaa
     7081 gtagtcgccg aacgagatga tgtagatcgc gatgtcgtcg cggataccgt tcaggcgcgg
     7141 cgtcagctcc agcccggcct tatagatgaa tgggcggatg tagatgttcg agcgcgggtt
     7201 gttctgcgca accaggtcct ggatgacggt ggcgagccca tccgcatcga gcttgagcga
     7261 cgagcgcagg atccggctgc tgttcatcag ccgccgggtg tggtccggca ggcggaagat
     7321 gttgatcgac tcctcggaca catccaggta gccacgaatg ccaccgaacg caccggtccc
     7381 gtactggagc gcgtgggtcg cgatgctgac cttcgcctcg tcggagggaa tgatctgccc
     7441 ctcgaaatac gcgtacggct gaagtggaat ctcggccacg accctctccc tctgctgggc
     7501 ttcgctgtac cccgatcctc gcgacgattc gaagacgggc gcattctagc acagcacttt
     7561 tacatcccct gtcgtcctga ggccacagcc gaagaacctc ttcgcctgac cgcaacacgg
     7621 ggttgacgcg gagaggttct tcggctgtga cctcaggacg acagcgcgac tcgttgacgc
     7681 gtacgatcac gtctccgtat aactgcccgg ctcatggaaa ggatgacggg tgggggacga
     7741 tcgacgattc tgggtcagct ttcatcggat ttccggcgtc ggctcggccc ggatcagccg
     7801 tctgctggaa tacttcgaca cgctcgaaga tgcctggaat gccggcgacc ggga
//
LOCUS       DASUGJ010000277         4593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_180433, whole genome shotgun sequence.
ACCESSION   DASUGJ010000277 DASUGJ010000000
VERSION     DASUGJ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180433"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="VFV93_06200"
     CDS             <1..1301
                     /locus_tag="VFV93_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5164969.1"
                     /translation="GSIGVSLLGNFDVTSPPQVMLDAFARLVHTRAPQIDVTAASDFI
                     DLIGLANLCGHGDVLPTSCPGEYTRPLLPEIRGQIAGTGPVELPPPIRREWLDLLDCQ
                     IGPATVYQGNLLEVRVTVQNAGIIPLETSGAPPGYIYDESQNYETAGYPKVSDAYRFA
                     LDFEGNSGLVNPFRWGFGSTLKPGEEREVVGYVRMKNLHSRTFSVSIVKEFVQYLTEN
                     AFEKRVQIVSPPVTRVPASSESGARYFSETGHNVPPTFDAYWSAYGGLRRFGYPLTEA
                     FEEVSETDGGVYLTQYFERARFELHPEYAGTEYEVQLGLVGVETTTERTAEDAFRPIA
                     AVASTADRFYFPETRHSLGGFFKRAWEDRGGLKIFGYPISEEFQELSETDGVVHIVQY
                     FERNRFEYHPDYAGTDDEVMLGHLAREILIRRGWLNGALT"
     gene            1394..2098
                     /locus_tag="VFV93_06205"
     CDS             1394..2098
                     /locus_tag="VFV93_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5164970.1"
                     /translation="MHERARQATVPPPAARLRSRVFLRVAIGAFLAFFGLLLVVRRNP
                     RMERDVVATVRVQRLHHPLLTGAMRVVSWFGFRPQSLVLPAATVSGTWLIGFRREARY
                     LCFAWLGSMLSYTTKRFVRRPRPSGEGIIVQDADLRDSSFPSGHTLHYTAFWGFFAYL
                     CFTEIRGRWLRWLPVSSIGSLIGAVGPSRIYLGHHWLTDVLASYSLGVSYLATLIGLH
                     RRKLGDQTPWREVNEG"
     gene            complement(2181..3941)
                     /locus_tag="VFV93_06210"
     CDS             complement(2181..3941)
                     /locus_tag="VFV93_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5164971.1"
                     /translation="MRRILAAILVASFMLTGLGQWSSPVAAVQEHDPEIVETLSMINT
                     YRSWLGIPPLTIDPSLQKAAEAHIEYYRLNFGDPSLAGMGLHYETPGKPGFTGADFQD
                     RADAAGYDGWVNENAGLSGSMVWSTEWFIGTVGHRLTLLDPRYSHIGLAAIDQGDIKF
                     EIIDLGTPKWVEDTTPEWAAWPPAGTTGVGLSFDGEAPNPFPSASYPIGYPISLKYFG
                     PGELTFSVATISTAGRAINSFAEIGTGWLSRETVLLCASEPLEMDTTYDVHIEGTANG
                     QPFVKDWSFRTTNGDDELANSAQTVAPPPATPSPSPTPTPEPTPAPGAPVELLPAGLK
                     ATDPLVQGLWWETDGPVAQLQSQRSWLWGPDTWKAAGERYAEEADGSRQVYYFDKARM
                     EVNERSGANKMVTAGLLVRDMIYGKAQVGDDQFVDAEPANVPLTGDPLEFNPDAPTYA
                     SLNGIASIETDRSVPARPGQPIVEVLWKDGSVGTNPSLGSIQYGSYEPTLGHNIAAVF
                     DTYLQSLATDWHMSVGLPLAEPYWVRTNLAGQPTWVLVQAFERRILTYTPINRPEWRV
                     EMGNVGRHYFTWRYGEEPPK"
     gene            4174..>4593
                     /locus_tag="VFV93_06215"
     CDS             4174..>4593
                     /locus_tag="VFV93_06215"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5164972.1"
                     /translation="MAQRHGDHPGAAQAGHDHGAGFWVQPEEARQADELAFQPAFYDI
                     DKLPTLAPVAGIKMSVMVGGAMMANWVRIEPGAAVPTHAHEHEQIGLVLEGEILMTIG
                     DETRALVPGHAYTIPGNLPHAAVAGPQGCLVLDIFSPP"
BASE COUNT          765 a         1472 c         1526 g          830 t
ORIGIN      
        1 acggctcgat cggcgtctcg ctgctgggta acttcgacgt gaccagcccg ccgcaggtga
       61 tgctcgacgc gttcgccagg ctggtgcata cccgcgcgcc gcagatcgac gtgacggcgg
      121 cgtctgactt catcgacctg atcggactgg cgaatctctg tgggcacggc gacgtcctgc
      181 cgacctcgtg tccgggcgag tacacacggc cgctgctgcc cgagattcgc gggcagatcg
      241 ccggaaccgg gccggtcgaa ctgccaccgc cgatccggcg cgagtggctc gacctgctgg
      301 actgtcagat cggcccggcg acggtctacc agggcaacct gcttgaggtt cgtgtgaccg
      361 tccaaaacgc cggcattatt ccgctggaga catccggcgc gcctcccggc tacatctacg
      421 atgagtcgca gaactacgag acggccggct acccgaaggt cagcgacgcc taccgctttg
      481 cgctggattt cgaagggaac agcgggctcg tgaacccgtt ccgctgggga ttcggctcga
      541 cgctcaagcc gggcgaggaa cgggaagtcg taggctacgt ccggatgaag aatctccatt
      601 cacgaacgtt ctcggtcagc atcgtcaaag agttcgttca gtacctgact gagaacgcct
      661 tcgagaagcg ggtccagatc gtctcgccgc cggtcacgcg cgttccggca tcgtccgagt
      721 cgggggcgcg ttatttctcc gaaaccggac ataatgtccc gccgacattc gatgcgtact
      781 ggtcggcgta tggcggctta cgccggttcg gctatccgct gaccgaagca tttgaggaag
      841 tctcggagac cgacggtggc gtctacctga cgcaatactt tgagcgggca cgtttcgagc
      901 tgcacccgga gtacgccggg accgagtacg aggtccagct tggcctggtc ggggtcgaga
      961 cgacgaccga acgcaccgct gaggatgcct tccggcctat tgcggcggtc gcgtcgacgg
     1021 ctgaccgctt ctacttcccc gagacgcggc acagcctcgg cggcttcttc aagcgcgcct
     1081 gggaggatcg aggcgggttg aagatcttcg gctatccgat ctcggaagag ttccaggagc
     1141 tgtcggagac ggatggtgtc gtccacatcg tccagtactt cgagcgcaac cgatttgagt
     1201 accaccccga ctatgccggc accgacgacg aggtcatgct cggtcatctg gcgcgcgaga
     1261 ttctgatccg gcgtggctgg ctgaacggtg cgctgacctg acaccgcccg cacgggtaca
     1321 atcccgtgcc gggagggatg cagtgagaca cgccagccgg tcggcggcca gagccgaaac
     1381 acttacggaa cagttgcacg agcgagcacg gcaggcgacc gtcccgccgc cggccgcgcg
     1441 attacgcagc cgcgtcttcc tgcgggtcgc gatcggggcc tttctggcct tcttcgggct
     1501 gttgctggtc gtcaggcgca atccccggat ggagcgtgac gtcgtcgcga ccgtccgcgt
     1561 ccaacggctg caccacccgc tgctgaccgg cgcgatgcgg gtcgtctcgt ggttcggctt
     1621 ccgcccgcaa tcgctggtcc tgccggcggc gaccgtgtcg ggcacctggc tgatcggctt
     1681 ccggcgcgag gcgcgctatc tctgcttcgc ctggctcggc tcgatgctct cgtacacgac
     1741 caaacgcttc gtgcgtcgcc cgcgtccctc cggcgagggc atcatcgtcc aggacgccga
     1801 cctgcgcgac tccagcttcc ccagcggcca tacgctgcac tacacggcct tttggggctt
     1861 cttcgcctac ctttgcttca cggagatacg cgggcgctgg ctgcgctggc tgccagtctc
     1921 gtcgatcggc tcgctgatcg gcgcggttgg gccgtcgcgg atctacctcg gccaccactg
     1981 gctgacggac gtgctggcca gctactcgct cggcgtcagc tacctggcga cgctgatcgg
     2041 actccaccgc cgaaagctcg gcgaccagac accatggcgt gaggtcaacg agggctgaca
     2101 cagaattcat ttcaaatcgg cagaacacgg catcacggaa gaggccgtga tgccgtgttc
     2161 tgcaaaggcc gaaacagtaa ctacttcggc ggttcctcgc cgtagcgcca ggtgaagtag
     2221 tgccggccga cgttgcccat ctcgacgcgc cactcggggc ggttgatcgg cgtgtaggtc
     2281 aggatgcggc gctcgaaggc ctggacgagc acccaggtcg gttgaccggc cagattcgtg
     2341 cgcacccagt acggctcggc cagcggcagg ccgacgctca tgtgccagtc ggttgccagt
     2401 gactggaggt aggtgtcgaa gacggccgcg atgttgtgac ccagcgtcgg ttcgtagctg
     2461 ccgtactgga tcgagccgag cgacgggttc gtcccgaccg agccgtcctt ccagagcacc
     2521 tcgacgatcg gctggccggg ccgggccggg actgagcgat cggtctcgat cgaggcgatg
     2581 ccgttcaggc tggcgtaggt cggcgcgtcg gggttgaact ccagcggatc gccggtcagt
     2641 gggacgttgg ccggctcggc gtcgacgaac tgatcgtcac cgacttgagc cttgccgtag
     2701 atcatgtcgc gaacgagcag accggccgtg accatcttgt tcgcgccgct gcgctcgttg
     2761 acttccatgc gggctttgtc aaagtagtag acctgtcgcg aaccgtcggc ttcctcggcg
     2821 tagcgttcgc ccgctgcctt ccaggtgtcc ggtccccaca gccacgagcg ttgggactgt
     2881 agctgcgcga ctggaccgtc cgtctcccac cagaggccct gaacgagcgg atcggtcgcc
     2941 ttcaggcccg ccggaagcag ctcgaccggc gcgccgggcg cgggcgttgg ttcaggcgtc
     3001 ggagtcggcg atggcgatgg tgttgccggc ggtggcgcga cggtctgcgc gctgttggcc
     3061 agttcgtcgt cgccgttggt cgtccggaac gaccagtctt tgacgaatgg ctggccgttg
     3121 gcggttccct cgatgtggac atcgtaggtc gtgtccatct ccagcggctc cgacgcgcag
     3181 agcaggaccg tctcgcgcga gagccagccc gtgccgatct ccgcgaaaga attgatggcg
     3241 cggccagcgg tcgagatggt tgcgacgctg aacgtcagct caccggggcc gaaatacttg
     3301 agcgagattg gataaccgat cgggtagctg gccgatggga acgggtttgg tgcctcgccg
     3361 tcgaagctca agccgacacc ggtcgtcccg gccggcggcc acgcggccca ctcgggcgtc
     3421 gtgtcctcaa cccacttcgg cgtgccgagg tcgatgatct cgaacttgat gtcgccctga
     3481 tcgatggccg ccagcccgat gtggctgtag cgcgggtcga ggagcgtcag ccggtggccg
     3541 accgtcccga tgaaccactc agttgaccag accatcgagc cggagagacc ggcgttctcg
     3601 ttgacccagc cgtcgtaacc ggcggcgtct gcgcggtcct ggaagtcggc gccagtgaag
     3661 ccgggcttgc cgggcgtctc gtagtgcagc cccatgccgg ccagcgacgg atcgccgaag
     3721 ttcagccggt agtattcgat gtgcgcctcg gctgctttct ggagggaagg gtcgatcgtc
     3781 agtggcggaa tgcccagcca gctgcgatag gtgttgatca tcgacagtgt ctcgacgatc
     3841 tcgggatcgt gttcctggac tgcggctaca ggcgaagacc actgaccaag gccggtcagc
     3901 atgaatgagg caaccagaat agccgccagg attcgacgca ttctcacagt gtgcaccttc
     3961 tcggtgtccg aaccgtaccg ggtgtaactg actggtgcga tatcccgggc agccacatcg
     4021 taacccaggc gaccgacgcg caacctgtgc ggatagtcct gaacatgtga taggaccggc
     4081 ctatcagcgg ccatgatcgt tcgcgctccg cgattgaatc gcctatgatg ccgcaagaca
     4141 gccgccagtg cacgaaggaa ggatggggct tcaatggcac agcgacacgg tgatcatccc
     4201 ggcgcggcgc aggccggcca tgaccacggg gccggtttct gggtccagcc ggaggaagcg
     4261 cgacaggccg atgagctcgc gtttcaaccc gcgttctacg acatcgacaa actgccaact
     4321 ctcgcgccgg tcgcaggcat caagatgagc gtcatggtcg gtggcgcgat gatggccaac
     4381 tgggtccgga tcgagccggg cgcggctgtg ccgacccacg cccacgagca cgagcagatc
     4441 ggcctcgtcc tcgaaggcga gatcctgatg acgatcggtg atgagacgcg cgcgctcgtg
     4501 ccggggcatg cctacacgat ccccggcaac ctgccacacg cggcggtggc cggcccgcag
     4561 ggctgcctgg tcctcgatat cttcagcccg cca
//
LOCUS       DASUGJ010000278         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1986926, whole genome shotgun sequence.
ACCESSION   DASUGJ010000278 DASUGJ010000000
VERSION     DASUGJ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986926"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VFV93_06220"
     CDS             <1..1152
                     /locus_tag="VFV93_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5164973.1"
                     /translation="DWLISRQRYWGTPIPIIYCDNCGMVPVPYEDLPVVLPEDSQFTP
                     SGKSPLQDDETFLNVACPSCGGPGRREADTMDTFMDSSWYWYRYLNPHYEEGPFDPAM
                     KPWVPVDQYTGGIEHATMHLLYARFFTKAMRDIGMVDFDEPFLRLFNQGIILGEDSEK
                     MSKSRGNVIDPDDLVHEYGADTVRLFLMFLGPWDQGGPWNSRGIAGPQRFLERAFTVI
                     TETAGNPAEARDDDATRALRRVTHQTIGGVTADIEVFAFNTAIAKLMEFVNELMKAKD
                     TPVASTAAWREAAESLALMLAPFTPHLAEELWQRLGKPFSVHTQTWPTWDEELAAAET
                     IEVAVQVNGKVRDRIELPAETTDADALAAARASARVQEYLTGKQVVKEI"
     gene            complement(1267..1800)
                     /locus_tag="VFV93_06225"
     CDS             complement(1267..1800)
                     /locus_tag="VFV93_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5164974.1"
                     /translation="MPDRTPIDVTNLDGYGNPPLEWSRAHALLSAPPDPDNPAGDSTI
                     FLGTIRPDGRPHSAGIGALWHDGDYYIVSGPETQKSRNLAANPACTISLSLKGMDLIL
                     EGDALRVTDTPTLETLAARYRDAGWPAQAEGDAFTAPYSAPSAGPPPWYLYRFTIHTA
                     YGVAGAEPPGATRWRFE"
     gene            complement(1908..2384)
                     /locus_tag="VFV93_06230"
     CDS             complement(1908..2384)
                     /locus_tag="VFV93_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5164975.1"
                     /translation="MGKTQITAEPGTPFLQITREFDAPRELVFRAYTEPDLLVQWLGP
                     RGIEMEVDRYDIRDGGSWRYIHRDAAGNEYGFHGVFHGTPSVDGIVQTFEFEGVPGHV
                     MLEWLTFEEHNGKTTIRGNSVAQSVEDRDAMVASGMESGVVEGYERLDELLERLAS"
     gene            complement(2420..2746)
                     /locus_tag="VFV93_06235"
     CDS             complement(2420..2746)
                     /locus_tag="VFV93_06235"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5164976.1"
                     /translation="MQSDQLTAVFGALADPTRRAILARLTEGDATVAELAAPFSVSQP
                     AISKHLKVLERAGLISRQRRATARISHLEAEPLRVATAWLAGYREYWDESFDRLDELL
                     AKLQQN"
BASE COUNT          485 a          920 c          978 g          479 t
ORIGIN      
        1 gactggctga tctcgcgcca gcgctactgg gggacgccga tcccgatcat ctactgcgac
       61 aactgcggga tggtcccggt tccgtacgag gatctgccgg tcgtcctgcc ggaggactcc
      121 cagttcacgc cgagcggcaa gtcgccgctg caggacgacg agacgttcct gaacgtcgcc
      181 tgccccagct gcggcgggcc gggccggcgc gaggcggata cgatggacac cttcatggac
      241 tcgtcgtggt actggtatcg ctacctgaac ccccactacg aggagggacc gttcgacccg
      301 gccatgaagc cgtgggtgcc ggtggaccag tacaccggtg ggatcgaaca cgcgacgatg
      361 cacctgctct acgcacgctt cttcacgaag gcgatgcggg acatcggcat ggtcgacttc
      421 gacgagccgt tcctgcggct gttcaaccag gggatcatcc tgggcgagga ctctgaaaag
      481 atgtccaagt cgcgcgggaa cgtgatcgac ccggacgatc tggtccacga gtacggcgcc
      541 gacacggtcc ggctgttcct gatgttcctg ggtccgtggg accagggcgg tccgtggaac
      601 tcacgcggca tcgccggtcc gcagcgcttc ctggagcgcg ccttcaccgt catcaccgag
      661 acggccggca atcccgccga ggcccgcgac gacgacgcaa cccgcgccct gcgccgcgtc
      721 acgcaccaga cgatcggcgg cgtgacggcc gacatcgagg tcttcgcctt caacacggcc
      781 atcgccaagc tgatggagtt cgtcaacgag ctgatgaagg ccaaggacac gccggtcgcc
      841 agcaccgcgg cctggcgcga agcggccgag tcgctggcgc tgatgctggc gcccttcacg
      901 ccgcacctgg ccgaggagct gtggcaacgg ctgggcaagc cgttctcggt ccacacccag
      961 acatggccaa cctgggacga agagctggca gccgccgaga cgatcgaggt ggcggttcag
     1021 gtcaacggca aggtccgcga ccgcatcgag ctgccggccg aaacaaccga cgccgacgcg
     1081 ctggccgccg cccgcgccag cgccagggtc caggagtacc tgaccggcaa gcaggtcgtg
     1141 aaggagatct aggttccggg caggctgatc agcttcgtcg tgcggtagtt cgggattcgg
     1201 gaaccgggac tcgggggaga gcaaagccgt tcgctcctcc cgaatcccgt gtcccgaatc
     1261 ccgatctcac tcaaagcgcc agcgcgtcgc gccaggcggt tcggcgccgg cgacgccgta
     1321 ggcggtgtgg atggtgaagc ggtagaggta ccaggggggt gggccagcgc ttggggcact
     1381 gtagggggcg gtgaaggcgt cgccctcggc ctgggcgggc cagccggcgt cgcggtagcg
     1441 ggcggccagc gtctcaagcg taggtgtgtc ggtgactctc agcgcatcgc cttcgaggat
     1501 caggtccatc cctttgaggc tgagcgagat cgtgcaggcc ggatttgcgg ccaggttgcg
     1561 tgacttctgt gtctccgggc cgctgacgat gtagtagtcg ccatcatgcc agagagcgcc
     1621 gatcccggcc gagtgcgggc ggccgtcggg ccggatcgtt ccgagaaaga tggtgctgtc
     1681 gccggccggg ttgtcggggt ccggcggggc ggacagcagg gcgtgcgccc ggctccactc
     1741 cagcggggga ttgccgtagc cgtcgaggtt cgtgacgtca atgggtgtgc gatcgggcat
     1801 cggtgtcacc tctgcttccc aattgcgagc gacgttgccg tgaaacagga gtatcgtctc
     1861 gctggcccca gcacgtagcg ggccagcgag aggatatgcg atctgcgcta gctggccagc
     1921 cgttcgagca gctcgtccag ccgttcgtag ccctcgacga cgccggattc catccccgag
     1981 gcgaccatcg cgtcgcgatc ctcgaccgac tgggcgaccg agttgccgcg aatggtcgtc
     2041 ttgccgttgt gctcctcgaa ggtcagccac tccagcatga cgtgtcccgg cacaccctcg
     2101 aactcgaagg tctggacgat cccgtcgacc gacggcgtcc cgtggaagac gccatggaag
     2161 ccgtactcgt tgccggcggc gtcgcggtgg atgtagcgcc agctgccgcc gtcgcggatg
     2221 tcgtagcgat cgacctccat ctcgatcccg cgcgggccaa gccactggac gagcaggtcc
     2281 ggctcggtgt aggcgcgaaa gaccagctcg cgcggagcgt caaactcgcg ggtgatctgc
     2341 aggaagggtg tgcctggctc ggcggtgatc tgcgtcttcc ccattgaagc atcctttcgt
     2401 cgtcgaaccg tcaacgcggt caattctgct gcaacttcgc cagcagctcg tcgaggcggt
     2461 cgaagctctc atcccagtac tcccgatagc cggccagcca ggcagtcgcc acgcgcaacg
     2521 gctcggcttc caggtgactg atccgtgccg tcgcccggcg ttgacgcgag atgagcccgg
     2581 ccctttccag caccttgaga tgcttcgaga tggccggctg tgagacgctg aacggcgcgg
     2641 caagttccgc gacggtcgcg tccccctcgg tcaggcgcgc cagaatcgcc cggcgggtcg
     2701 gatcggcaag cgccccaaag acggcagtga gctggtcgga ttgcattcgt aacgacccca
     2761 ttccaaaacc aataggttat agaataggat gtagggatgc gtttgtcaac aggtgagcag
     2821 ctacaggaaa gtaggggccg tgccttgtgc cggccctgct tc
//
LOCUS       DASUGJ010000279         6532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1847512, whole genome shotgun sequence.
ACCESSION   DASUGJ010000279 DASUGJ010000000
VERSION     DASUGJ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847512"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFV93_06240"
     CDS             complement(<1..482)
                     /locus_tag="VFV93_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164977.1"
                     /translation="MQDDNQERTDQPARRQQSRVWAEERGDGQVLLTWQRADPPAEDA
                     DAAADARAVAATIGVLDGVSATSAAEGGVLVTFDPKAISRQQLAGGVRAALNLDADLK
                     TRGNELMKRVPAYLGLAKALALDERVSPVPEAARQAAAARGTSMRAAGALPVATRFIP
                     G"
     gene            623..1324
                     /gene="rpiA"
                     /locus_tag="VFV93_06245"
     CDS             623..1324
                     /gene="rpiA"
                     /locus_tag="VFV93_06245"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX5164978.1"
                     /translation="MSSVESGEEQKRRAAERAVELVENGMALGLGSGTTAELVVRALA
                     PRVAGGLRLRCVATSGRTEQLARDLGITLHELDGELDLALDGADAIERGTLAAIKGRG
                     GALTREKIVALAARRFVLVGDASKPVDHLRDLLPAMPVPVEILPFGWQLTRTRLAILG
                     DPIVRTRSGSPFVTDNGNYILDLYNAALDRPADLAAAIEAVPGVVEHGLFLDIATLAL
                     IGGPDGVVELRRGGA"
     gene            complement(1328..2779)
                     /locus_tag="VFV93_06250"
     CDS             complement(1328..2779)
                     /locus_tag="VFV93_06250"
                     /inference="COORDINATES: protein motif:HMM:NF004801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase family protein"
                     /protein_id="HEX5164979.1"
                     /translation="MTSAPVNRERIRIDGGVIVAFQDGGHRILTDGVVVVEGNEVVHV
                     GKTFDGQADRVIDARNAIVTPGLINTHAHLTESPLDKSFIEDQGRRQFYYSGLFEMLP
                     ARSAGIDDDGRRACVDYSMGELLRTGTTTIMEIGGFGDYTAEAAEKAGLRAYIANSYR
                     SGRWYTPDGRQVAYRWDEETGRQGFRNAVEFIERVDGAANGRIKGYLSPAQVDTCTEE
                     LLRMSREASDSMQVPLALHTSQSTNEFQEMIHRNGLTPVEWLNEIGFLSEWNILGHVI
                     IIAGSSWAQFSGNDLRLLADAGSSVAHCVWVFARRGIAMESFGGYLKAGVNMCLGTDT
                     APQSMIEALRWTAVIGKIMARETSQSTAADVFNAATLNAARMLHRDDLGRIAPGAKAD
                     ILIWQRDSLFMTPLRDALKNIVYNAQTEDLRTVMIDGAIVLDDGQPLYVDQAAANQAV
                     QHAGERMWSEMQAGDWARRSVEELSPMTFPAWK"
     gene            2923..3240
                     /locus_tag="VFV93_06255"
     CDS             2923..3240
                     /locus_tag="VFV93_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164980.1"
                     /translation="MAETPAAQPFDTGRNEALRHVRFEDLLRAVGSYIDQHGFTDVLV
                     TQLPEGILLKGTVIDRTKRSANERIAAVLFTNDDVLKLLDASARRRGSTDQLRSRWMQ
                     QQS"
     gene            3335..4189
                     /locus_tag="VFV93_06260"
     CDS             3335..4189
                     /locus_tag="VFV93_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5164981.1"
                     /translation="MAISHPLRVGVVSGARKPYPELVQRWKDAESLGFDTVWVTDHFI
                     TGNEPENDAEPLLEAWTVIAGLAMATERIRFGVMVTGNTYRNPALLAKQAVTVDHISN
                     GRLELGFGAGWWVREHEAYGYPMPGNRELVDRFGEALTIIDGLQKNERFDFHGQYYFV
                     TDAPFEPKPVQQPNIPMLVGASGPRMLRLTAKFANIWNTRGPVDVAAERNQLLTEKCH
                     EVGRDPGEIRRSIWPYEHPWESIDNVKQVIESYREVGYTDFVFGWPPDEYTGVMVEFA
                     RNVMPGLR"
     gene            4265..4918
                     /locus_tag="VFV93_06265"
     CDS             4265..4918
                     /locus_tag="VFV93_06265"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5164982.1"
                     /translation="MTSTHKIIDISIPIQPGMPQWPGDDPVTERPLSRTPEDVANVTQ
                     LVLTTHTGTHVDPPRHFVHDGATIDEVPLERWIGRCWVADLTGSRPEVEVAELEAAGI
                     PDGTQRLLLKTTNTDLWRSRPAAFVEQYVALSLPAAGWVVERGIRLIGIDYLSIGGMR
                     TTNVETHQLLLGNDVIVVEGLDLVDVEPGPYELLCMPLKIRAGDGAPARVALRGPLG"
     gene            complement(4922..6028)
                     /locus_tag="VFV93_06270"
     CDS             complement(4922..6028)
                     /locus_tag="VFV93_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX5164983.1"
                     /translation="MREMRAAVFYEYNQPMVIETIQIDPPQAGEVLVQIAASGVCRSD
                     LHVLKQEWTAPLPIVLGHEASGRVVEVGAGVTRVATGDPVVLSFAPNCGRCDYCASGR
                     GHLCSTMRREPSGLLPGGTSRLHMDDQPINIFARMGSFAEYAVVHESSVVPISPDVPL
                     DLAALIGCSVTTGIGAVLNTARVPAGSTVVVIGCGGVGLNVIQGARLVSARRIIAVDI
                     SLEKLELAQRCGATDLIDARDIEVAKHIRDLTDDGVDFAFEALGSSPTIQAAYDVVRP
                     GGTAVVAGMAPYGEMVPINAFLLAYQEKTLKGSYYGSARTSLDMPLLVHLAQTGQIEL
                     DSLVARRYPLEEINEAYRDLDQGESGRGLIVFDD"
     gene            complement(6123..>6532)
                     /locus_tag="VFV93_06275"
     CDS             complement(6123..>6532)
                     /locus_tag="VFV93_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5164984.1"
                     /translation="VHQFSTFATATPFQEAMAVALETAEERGYYRQLRDEYSQRRDRL
                     RAVLADVGLPPLDCQGAYFLLSDVTPHGFDNDIDFCRYLVTEAGVAAIPPSAFYLDPT
                     TAPPLARFCFAKRFETIDAAAERLGRLRQPGSV"
BASE COUNT         1090 a         2125 c         2198 g         1119 t
ORIGIN      
        1 cctgggatga atctggtcgc gaccggcaac gccccagccg ctcgcatcga tgtcccgcgg
       61 gcggcggcgg cttgccgggc tgcttcgggg accgggctaa cgcgctcgtc gagagccagc
      121 gccttcgcca ggccgagata ggccgggacg cgcttcatca gctcgttgcc gcgtgtcttc
      181 agatcggcgt cgagattcaa cgcggcccgg acgccgccgg cgagttgctg gcggctgatc
      241 gccttgggat cgaacgtgac cagcacgccg ccctcggcag cgctcgtggc gctgactcca
      301 tccagaacgc caatcgtcgc ggcgacggcg cgggcgtcgg ctgccgcatc ggcgtcctcc
      361 gccggcgggt cggcacgttg ccaggtcagc aggacctggc cgtcgccacg ctcctcggcc
      421 cagacgcgtg actgttgccg gcgtgcgggc tggtccgttc gctcctgatt gtcgtcctgc
      481 accttgcacc ctcgattcca gcgtcgtccc gtcaaatggt acccgccgat ggcaccagct
      541 tgcgatggcg cgcgcaatca gcaactattc ggagcgcgtc cgggaatggc cgggcagcgg
      601 gatgtcgaaa gggaacgacc aggtgtcgag cgtggagagc ggcgaagagc agaagcggcg
      661 cgccgctgaa cgggcggtcg agctggtcga aaacggcatg gcgctcggtc tcggcagcgg
      721 tacgacggca gaactcgtcg tacgcgcgct ggcgccccgt gtcgctggag ggctgcggct
      781 gcgttgcgtg gcgacgtcgg gccgcacgga gcagcttgcc cgcgacctcg gcatcacatt
      841 gcacgagctt gacggcgagc tcgatctggc gctggacggc gctgacgcga tcgaacgcgg
      901 cacgctggca gccatcaagg ggcggggcgg ggcgttgacg cgcgagaaga tcgtcgcgct
      961 ggcggcccgg cgcttcgtcc tggtaggcga tgccagcaag ccggtcgacc acctgcgcga
     1021 tctcctgccg gcaatgccgg tgccggtcga gatcctgccc ttcggctggc agctgacccg
     1081 gacgcggctg gcgatcctcg gtgacccgat tgtccgcacc cgctccggaa gcccgttcgt
     1141 gaccgacaac ggcaactaca tcctcgacct ctacaacgct gcgcttgatc gcccggccga
     1201 cctcgcagcg gcgattgagg ccgtgcccgg tgtcgtcgag cacggcctgt ttctcgacat
     1261 cgccacgctg gccctcattg gcgggccaga cggcgttgtc gagttgcgtc gtggcggtgc
     1321 ctgatcgcta cttccaggcc gggaatgtca tcggcgacag ctcctcgacc gatcgacgcg
     1381 cccagtcgcc ggcctgcatc tccgaccaca tccgctctcc ggcgtgctgg acagcctggt
     1441 tggcggctgc ctgatcgacg tagagtggct gaccgtcgtc gaggacgatg gcgccgtcga
     1501 tcatgaccgt tcgcagatcc tcggtctgcg cgttgtagac gatattcttg agggcgtcgc
     1561 gcagcggcgt cataaagagc gagtcgcgct gccagatcag gatatcggcc ttggcgcccg
     1621 gcgcgatcct tcccagatcg tcgcggtgca gcattctggc agcattgagc gtcgcggcgt
     1681 tgaacacatc agccgccgtg gactggctcg tctcgcgggc catgatcttg ccgatgacgg
     1741 ccgtccagcg caacgcttcg atcatcgact ggggagcagt gtcggtgccg aggcacatgt
     1801 tgacgccggc tttcagatag ccgccgaacg actccatcgc gatcccgcgc cgcgcgaaga
     1861 cccagacgca gtgtgcgacg ctggacccgg catccgccag caaacgcagg tcgttgccgc
     1921 tgaattgtgc ccacgacgat cccgcgatga tgatgacgtg cccgaggatg ttccactcgc
     1981 tgaggaaacc gatctcgttc agccactcga ccggcgtcaa cccgttgcga tggatcatct
     2041 cctgaaactc gttcgtggat tggctcgtat gcagcgccag cggcacctgc atgctgtcgg
     2101 acgcctcgcg gctcatccgc aacagctctt cggtgcaggt gtcgacctgg gctggcgaga
     2161 ggtagccctt gatgcggccg ttggcggcgc cgtcgacccg ctcgatgaac tcgactgcgt
     2221 tgcggaagcc ctgcctgccg gtttcctcat cccagcggta agcgacctgc cgcccatccg
     2281 gcgtgtacca gcggcccgag cggtagctgt tggcgatgta ggcgcgtagg ccggccttct
     2341 cggctgcctc agccgtgtag tcgccgaagc cgccgatctc catgatggtc gtcgttccgg
     2401 tccgcaacag ctcgcccatc gagtaatcga cgcaggcgcg ccggccgtcg tcgtcgatac
     2461 cggccgagcg ggccggcagc atctcgaaca ggccggagta gtagaactgg cgccggccct
     2521 ggtcctcgat gaacgacttg tcgagcggcg actccgtcag gtgggcgtgg gtgttgatca
     2581 ggccgggtgt gacgatcgcg ttgcgtgcgt cgataacgcg gtcggcctgc ccgtcgaagg
     2641 tcttgccgac gtggacgacc tcgttgccct ccacgacgac gactccgtcg gtcaggatgc
     2701 gatgcccgcc gtcctgaaac gcgacgatga cgccgccgtc gatcctgatc cgctcccggt
     2761 taactggcgc actggtcatg aatgctctcc cctcgtcctc acgattccga atgcgccgag
     2821 agggtagcac agcgattgct gccggccggc aacgtttggc aaccggcgtg cgagcgcgta
     2881 cgatagcgcg aatccgtgcg tgaatgcgat gggagccgtg gtatggccga gacgccagca
     2941 gcgcagccgt tcgatactgg ccggaacgag gcactgcgcc acgtccgttt tgaagatctg
     3001 ctgcgcgccg tcggcagcta tatcgatcag catgggttca ccgatgtgct ggtgacgcag
     3061 cttccggaag ggattctcct caagggaact gtcatcgacc gcacgaagcg atcggctaat
     3121 gaacggatcg cggccgtcct gttcacgaac gatgatgtcc tgaagttgct cgacgccagc
     3181 gccaggcggc gcggctccac tgaccagctc cgatcgcgct ggatgcagca gcaatcctga
     3241 gtcggtaaca gacgtttcga gagaatacgc ggcagccagg tcgggttgcc gtagaatcgg
     3301 cgcggcgagt tgcgccgaca agagggagga atctgtggcg atcagccatc cgttgcgtgt
     3361 tggggtcgtg tccggcgcgc gaaagcctta tccggagctt gtccagcgct ggaaggacgc
     3421 cgagtcgctg ggattcgaca cggtctgggt gaccgaccac ttcatcaccg gcaacgagcc
     3481 ggagaacgac gccgagccgc ttctcgaagc atggacggtg atcgccgggc tggcgatggc
     3541 gaccgaacgc attcgcttcg gcgtgatggt gaccggcaac acctatcgca acccagccct
     3601 gctggcaaag caggcagtga cggtcgacca catctcgaac ggacgactgg agctgggctt
     3661 tggcgccggc tggtgggtgc gcgaacacga ggcgtatggc tacccgatgc cgggcaatcg
     3721 cgaactggtc gaccgattcg gcgaggcact gacgatcatc gacggcctcc agaagaacga
     3781 gcgcttcgat ttccacggcc agtattactt cgtcaccgat gcgccgttcg agccgaagcc
     3841 tgtccagcag ccgaacatcc cgatgctcgt cggcgcgtcc gggccccgga tgctgcggct
     3901 gacggcgaaa ttcgccaaca tctggaacac gcgcggaccg gtcgatgtcg ccgccgagcg
     3961 caaccagttg ctgaccgaga agtgtcacga ggtcggccgc gaccccggcg agatccgccg
     4021 ctcgatctgg ccctacgagc acccgtggga gtcgatcgac aacgtcaagc aggtcatcga
     4081 gtcgtaccgc gaggttggct acaccgactt cgtcttcggc tggccgcctg acgagtacac
     4141 cggcgtcatg gtcgagttcg cgcggaacgt catgccgggg ttgcgatagc tgccctcaac
     4201 cccgaacccc tcgcccaatc ttcggagtgg gggttggggt gagggtggtc caggagcgtc
     4261 accaatgacg agcactcaca aaatcatcga catcagcatc ccgatccagc cggggatgcc
     4321 gcagtggccg ggagacgatc cggtgaccga gcggccgttg tctcggacgc ccgaggatgt
     4381 cgcgaatgtg acgcaactgg tgctgaccac acacaccgga acgcacgtcg atccgccacg
     4441 ccacttcgtc cacgacggcg cgacgatcga cgaggtgccg ctggagcgct ggattgggcg
     4501 gtgctgggtt gcggacctga ccggctcgcg gccggaagtc gaggtggccg agctggaggc
     4561 agccggcatc ccggatggca cccagcgctt gctgctgaag acgaccaaca cggacctctg
     4621 gcgctcccgc ccggccgcat tcgtcgagca gtacgtcgcg ctctcactgc cggcggccgg
     4681 ctgggttgtc gagcgcggca tccggttgat cgggatcgac tacctctcga tcggcgggat
     4741 gcgcacgacc aacgttgaga cgcatcagct cctgctcggc aacgatgtca tcgtcgtcga
     4801 agggctggat ctggtcgatg tcgagccggg tccatatgaa ctgctgtgca tgccgctcaa
     4861 gatccgggcc ggcgacggcg cgccggcccg cgttgcgctg cgtgggccgc ttggttgatc
     4921 gctagtcgtc gaagacgatc aggccacggc ctgactcgcc ctggtcgagg tcgcggtacg
     4981 cctcgttgat ctcttccagt gggtagcggc gcgcgacaag cgaatccagc tcgatctggc
     5041 cggtctgggc gaggtggaca agcagcggca tgtcgagact tgttcgcgcc gagccgtagt
     5101 agctgccttt gagcgtcttc tcctggtacg ccagcaggaa cgcgttgatc ggcaccatct
     5161 ccccgtaagg ggccatcccg gcgacgacgg ccgtgccgcc cggtcgaacc acgtcgtagg
     5221 ccgcttgaat cgtcgggctg ctgccaagtg cttcgaatgc gaagtcgaca ccgtcgtcgg
     5281 tcaggtcgcg gatgtgtttc gccacctcga tgtcgcgggc atcgatcaga tcggtcgcgc
     5341 cgcagcgctg ggccagctcc agcttctcca ggctgatatc gacggcgatg atgcgccggg
     5401 ccgagacgag ccgcgcaccc tggatgacgt tgagcccgac accgccgcag ccgatgacga
     5461 caaccgtgct gccggccggc accctggctg tgttgaggac ggcgccgatt ccagtcgtga
     5521 ccgaacagcc gatcagggcc gccagatcga gcggaacgtc cggcgagatc gggacgaccg
     5581 agctctcgtg gacgactgcg tactcggcga acgagcccat tcgggcgaag atattgatcg
     5641 gttggtcgtc catgtgcagc cgcgatgtgc cacccggcag cagcccgctt ggctcgcggc
     5701 gcatcgtcga acagaggtgg ccccggccgg acgcgcagta gtcgcatctg ccgcagttcg
     5761 gcgcgaacga gaggacgacc ggatcgccgg tcgccacgcg cgtcacgccg gcgccgacct
     5821 cgacgacgcg ccctgacgcc tcgtgtccaa gcacgatcgg cagcggcgcg gtccactcct
     5881 gcttgagcac gtgcagatcg ctgcgacaga cgccgctggc ggcgatctgg accagcacct
     5941 cgcctgcttg cggcgggtcg atctggatgg tctcgatcac catcggctgg ttgtactcgt
     6001 agaagacggc agcacgcatc tcgcgcatgg gttcgctccc tccaacggtc tgaccgcggt
     6061 cgtagatggg aactcgcgaa cgatgcggtt atgtcgcgcg aattgtcgcc gttgtcaagt
     6121 aactagacgg aaccgggttg gcgcagcctg ccgagccgct cggcggcagc gtcgatcgtc
     6181 tcgaaccgct tggcgaagca gaagcgcgcc agcggcggcg ccgtcgtcgg atcgaggtag
     6241 aacgccgatg gcgggatcgc tgcaacgcct gcctcagtca cgagatagcg acagaagtcg
     6301 atgtcgttgt caaagccgtg cggcgtcacg tcgctgagca ggaagtaggc accctggcaa
     6361 tcgagcggcg gcaacccgac gtcggccaga acagcccgca gccggtcccg gcgctgcgaa
     6421 tactcatctc ggagctgccg gtaatagccg cgctcttcgg ccgtttcgag cgcgacggcc
     6481 attgcctcct gaaacggcgt cgccgtcgcg aaggtcgaga actggtggac ag
//
LOCUS       DASUGJ010000280         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1292755, whole genome shotgun sequence.
ACCESSION   DASUGJ010000280 DASUGJ010000000
VERSION     DASUGJ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292755"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..>2708
                     /locus_tag="VFV93_06280"
     CDS             328..>2708
                     /locus_tag="VFV93_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5164985.1"
                     /translation="MISVFAAAWRVILKRTGADWLILAAATLIILLSTTLLAAGPIYA
                     GAVTLSGLHRTLADAQVIEADVEISSRVRPENYRAMNDRVASEAGRAFAPTGATVERL
                     GRSDSFALPDQAQADVTDLAVFNFFENLQEHATLVDGTWPDGASTGPVGAVISDSTAG
                     LLDLSVGDELLLANRREGNFTVQTQIIGIYTVNDPSDPYWFQEELELAGVEVGQSFTT
                     YGPFVVSPEDFFGRTTYVSGEVFWRIFPTFTNLSVAEVGPFRQNIDRLGDGLNAGVDS
                     GQRFTIQTELNNILRKAERSLLVTRTGVMILTVQLAVLAGYALVLTAGLLIEQRRVET
                     ALLRSRGASNSQIAAMALMEGLLLALPAAIAGPWIAAFSLRLLNVIGPLTSIELHLDP
                     KVTTGSYLLSAISAIACVIALVLPAYLAARSFVAARAARGRQMMSGIAQRGGIDLALL
                     AVAILGYWQLRRYGAPITETVQGRLGLDPFLVAAPAIGLLAGAVVALRVIPLLAKLIE
                     RSVANSQSVVSSLGAWQVARRPLRYTRSALLLMLALAIGLFAVAYTRTWTDSQRDQAD
                     FQVGADMRLTPDRRTGRAIPQYTLVDAYRQIDGVQQPMPVMRNTAVISRSAGTGRVLG
                     VDAATADQVIQFRSDLSGNSLESMLGELEADRPELPMVELPGEPLRLALDIDVALDPL
                     PGDAPAEANQPIAPFVSVVIQDARGMLYRLTGGSLESDAGVQQLVIDLGFNLGESEAR
                     PEYPINLVAVEARILIPIQVPRTGRFELVTLQASDSVEGDDWLPVQLASDPAR"
BASE COUNT          423 a          810 c          983 g          492 t
ORIGIN      
        1 cctgccggcc catcgtgctg gtcatacgcg tgacatgcta ccagaagata cgcacagcca
       61 acccgcgctg gttgcaccct ctgtcgtcct gagccgcagc gaaggacctc tccgccccaa
      121 cgccatgtgc ggctgacggg agaggtcctt cgctgcggcc caggacgaca ggggggggtg
      181 caaccagcgc gggttggctg tgcgtatctt ctggtagcat gtcacgcgta tgaccagcac
      241 gatgggccgg cagggggagg cgaggtcggg atcagccacg gggaggctga cggctgatcg
      301 ctgacggctg atggctaaag ggctctcatg atctcggttt tcgccgctgc ctggcgggtg
      361 atcctcaagc gcaccggcgc tgactggctg atactggcag cggcgacgct gattatcctg
      421 ctttcgacga cattgctggc ggctggtccg atctacgccg gggcggtgac gctctcggga
      481 ttgcaccgga cgctggccga cgcacaggtc atcgaggccg acgtcgagat ctcctcgcgg
      541 gtgcgaccgg aaaattaccg ggcgatgaac gaccgcgtcg ccagcgaggc aggccgcgcg
      601 ttcgcgccaa ctggcgcgac ggtcgagcgg ctggggcgct ccgactcatt cgcgttgccg
      661 gaccaggccc aggccgacgt caccgatctg gcggtgttca acttctttga gaacctgcag
      721 gaacacgcga cgttggttga cggcacctgg ccggacggcg cgtcgaccgg gccggtcggg
      781 gcggtgatct ccgattcgac ggcggggctg ctggacctgt cggtcggcga tgagctgctg
      841 ctggcaaatc gccgtgaggg aaacttcacg gtccagacac agatcatcgg catctacacg
      901 gtcaacgatc cgtccgaccc gtactggttc caggaggagc tggaactggc cggcgtcgag
      961 gtcgggcagt cgttcacgac ctacgggccg ttcgtcgtct cgccggagga cttcttcggg
     1021 cggacgacct acgtctccgg cgaggtgttc tggcggatct tcccgacgtt cacgaacctg
     1081 agtgtggccg aagtcgggcc gtttcggcag aatatcgacc ggcttgggga tgggctgaac
     1141 gcgggtgtcg atagcgggca gcgcttcacg atccagaccg aactgaacaa catcctgcgc
     1201 aaggcggaac ggtcgctgct ggtgacgcgg acaggcgtca tgatcctgac cgtccagctg
     1261 gcggtgctgg ccggttacgc gctggtgctg acggccgggc tgctgatcga gcagcgacgg
     1321 gttgagacgg cgctgttgcg ttcgcggggg gctagcaaca gccagatcgc ggcgatggcg
     1381 ttgatggagg ggctgctgct ggcactgccg gcggcgatcg ccggaccgtg gatcgcggcc
     1441 ttctcgctgc ggctgttgaa cgtcatcgga ccgctgacat cgatcgaatt gcatctggac
     1501 ccgaaggtga cgaccggtag ctacctgctg tcggcgattt cggcaatcgc ctgtgtcatc
     1561 gcgctggtgc taccagccta cctggcggcg cgctcgttcg ttgcggcccg cgcggcgagg
     1621 ggccggcaga tgatgagcgg gattgcccag cggggcggga tcgacctggc gctgctggcg
     1681 gtagcgatcc tgggctactg gcagcttcgg cgctatggcg cgccgattac agagacggtc
     1741 caagggcggc tggggctcga cccgttcctg gtggcagcgc cggcgatcgg gttgctggcc
     1801 ggtgcggtcg tggcgctgcg ggtcatcccg ctgctggcca agctgatcga gcgatctgtg
     1861 gcgaacagcc agtcggtcgt ctcgtcgctg ggcgcctggc aggtggcacg gcggccgttg
     1921 cgctacactc gctcggcgtt gctgctgatg ctggcgctcg cgatcggatt gttcgccgtc
     1981 gcgtacaccc ggacgtggac cgattcacag cgcgatcagg ctgactttca ggttggcgcg
     2041 gacatgcggc tcacgccgga ccggcgaacg gggcgggcga tcccgcagta cacgctggtc
     2101 gacgcctacc ggcagataga cggcgtccag cagccaatgc cggtcatgcg caacaccgcc
     2161 gtgatctccc gttcggcagg cacgggccgg gtgctcgggg tggacgcggc gacggcggat
     2221 caggtcatcc agttccgctc ggatctctca ggcaattcgc tggagtcgat gctgggcgag
     2281 ctggaggccg atcgacccga gctgccaatg gtggagctgc caggcgagcc gttgcggctg
     2341 gcgctggata ttgacgttgc gctggacccg ttgccgggag acgcgccagc cgaggcgaat
     2401 cagccgattg ctccatttgt atcggtcgtc attcaggatg cgcgcgggat gctctaccgg
     2461 ctcacgggcg ggtcgctgga gtcggacgct ggtgtccagc agctggtgat cgatctgggg
     2521 ttcaatctgg gcgagagcga ggcaagaccg gagtatccga tcaacctcgt cgccgtcgag
     2581 gcaaggattc tgataccgat acaggtgccg cgaacgggcc gtttcgagtt ggtaacgttg
     2641 caggcgagtg attcggttga aggggatgac tggctgccgg tgcaacttgc gagcgaccca
     2701 gcacgctg
//
LOCUS       DASUGJ010000281         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_876816, whole genome shotgun sequence.
ACCESSION   DASUGJ010000281 DASUGJ010000000
VERSION     DASUGJ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876816"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..924)
                     /locus_tag="VFV93_06285"
     CDS             complement(28..924)
                     /locus_tag="VFV93_06285"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HEX5164986.1"
                     /translation="MTTPELLCQKFTVTPHDLELVRAAGAVLLPELSALIERWYEWLG
                     KQPEMTTFFDDASSIARVKHLQAAHWTAFFDGTIDESYIAHRRHVGAVHANIDLPNDS
                     YFAGIAVMLELFTVTLENKIGDRNQLTAISQAISKVIFLDAYLAVDEIARIQKERIAE
                     HSRAVMEMSTPVTPIWEGILLLPLVGVIDSMRTADIMNKSLTSIAEMRAKVFVLDISG
                     VGAVDTAVANQLIKITKATRLLGCEPIISGLSPSIARTMVELGVNVGEIRTTATLRDG
                     FEIALKMMGMLGGLTGQQKVAD"
     gene            complement(1256..>2833)
                     /locus_tag="VFV93_06290"
     CDS             complement(1256..>2833)
                     /locus_tag="VFV93_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5164987.1"
                     /translation="RPGSRYYRNQEWVHMVNDDIISMPDKWEYPWYAAWDLAFHTVAL
                     AVVDPDFAKKQLDLLLNANYLHPNGQIPAYEWAFGDVNPPVHAWASLFLTRIYQARGG
                     SLDTEFLAAAFHKLSLNFAWWANRKDRSGKNLFDGGFLGLDNISVFDRSAPLPTGGYL
                     EQADGTAWMAFFCQNMAELAIELAAHDGQVMYQGLAAKYVDRFLWIAAAMNRPGDGGM
                     WDEEDGFYYDVLRLPDGSATRLKVRSLVGLLPLCATTVIEPEQREAAQVAVAALQARV
                     TQLPELLNTIHLTGPGHRGVGDRGIIALLNPERLRRVLTRMLDENEFLSPHGIRSISR
                     FHADHPYTPPLGGSDYRVDYLPAESNTGMFGGNSNWRGPIWFPINTLIIRSLLSFYLY
                     YGDDFKVECPTGSGRWMNLFEVARDLAERLTGIFLRAPSEGGGRRPVYGWTEKFQTDP
                     HWRDLILFYEYFHGDNGAGVGASHQTGWTGLVAPLIQLFGSLDGQELLRTGKQGMFAR
                     AGVQPQPTGARSSVVAG"
BASE COUNT          497 a          915 c          886 g          535 t
ORIGIN      
        1 gtcatggcaa tccgtgcttc ccgttggtta gtcggccacc ttctgctggc cggtcagtcc
       61 gcccagcatc cccatcatct tgagcgcgat ctcgaagccg tcccgcaagg tagccgtcgt
      121 ccggatttcg ccgacattga ctcccagctc gaccatggtt cgcgcgatcg acggagatag
      181 tccggaaatg atgggttcgc accccaggag gcgcgttgcc ttcgtgatct tgatcaactg
      241 attagccacc gccgtgtcga cggcgccaac accactgata tcaagtacga agaccttggc
      301 gcgcatctcg gcgatcgagg tgagcgattt attcataatg tcggcggtac gcatagaatc
      361 gatgactccg accaggggaa ggagcaagat accctcccag atcggcgtta ccggcgtcga
      421 catctccatc accgcccggc tgtgttccgc aatacgctcc ttctggattc tcgcaatctc
      481 atcgaccgcc agatacgcgt cgagaaagat caccttcgat atggcctggg aaatcgccgt
      541 gagctgattc cgatcgccga ttttgttctc caacgtcacc gtgaaaagtt caagcattac
      601 cgcgatgcca gcgaagtaac tgtcgttcgg caagtcaata tttgcatgca ctgctcccac
      661 gtgccggcgg tgcgcaatgt agctctcatc gatcgtacca tcgaagaatg cagtccagtg
      721 cgccgcctgc agatgcttga ctcgtgcgat gctgcttgca tcgtcgaaga atgttgtcat
      781 ctctggctgc ttgcctagcc actcgtacca ccgttcgatc aaagcagata gctccggcag
      841 gagcaccgct cctgctgcgc gcacgagctc caggtcatgt ggggtaacgg tgaatttttg
      901 gcaaagcagc tctggtgtcg tcatggtgcg gggtccattc agcatctccg tatacctcag
      961 tggtacctac tggccgacgc ctgccgcgcc gggggtgggc actgcgtgag tcggcgccga
     1021 gcctcgcctt cgatcagtct cccgggctct ccgacgagca gtccgtgata gcgcccggta
     1081 ttcaccccgc acacggcgcc gctggcatag ccgtcgagac cgttggtagt gagccattcg
     1141 tgtgtatcga gatctcccat ggtgacactc cctgccccgg ccttagtctg ccatcctccc
     1201 ggcgctccag ggctgtcatc cgacagcgcc aggctgccgt tccacggcct cgcggtcagc
     1261 cggccaccac ggaactacgc gccccggttg gctgaggctg aacgccggcc cgggcgaaca
     1321 tcccttgctt gcccgtcctg aggagctctt ggccgtccag ggagccgaag agctggatca
     1381 gcggcgccac caagccggtc cagcccgtct gatggctggc gccgacgccc gcgccgttgt
     1441 cgccgtggaa gtactcgtag aagaggatca gatctctcca gtgcgggtcc gtctggaact
     1501 tctcggtcca gccgtagacc ggccgtcgcc cgccgccttc ggagggcgct cgcaagaaga
     1561 tcccggtgag ccgctcggcg agatccctgg ccacctcgaa caggttcatc cagcgcccgg
     1621 agccggtggg gcactcgacc ttgaagtcgt cgccgtagta caggtagaag ctcaggagcg
     1681 accggatgat caacgtgttg atcgggaacc agatcggccc gcgccagttg gagttgccgc
     1741 cgaacatccc ggtgttcgat tcggccggca gatagtcaac ccgatagtcc gagcccccca
     1801 gcggcggggt gtacggatgg tcggcatgga acctggagat cgagcggatc ccgtgggggc
     1861 tgaggaactc gttctcgtcg agcatccgcg tcagcacacg gcgcagccgt tccgggttga
     1921 ggagcgcgat gatgccgcgg tcgccgacgc cgcgatggcc tggcccggtt agatggatcg
     1981 tgttgagcag ctccggtaac tgcgtcacgc gggcctgcag cgcggcgacc gcgacctgag
     2041 ccgcctcgcg ctgctcgggc tcgatgacgg tggtggcgca cagcggcagc agcccgacca
     2101 gcgagcggac cttcagccgc gtggcgctgc cgtccggcag acggaggacg tcgtagtaga
     2161 aaccgtcctc ttcgtcccac atcccgccat cgccgggccg gttcatggcc gccgcgatcc
     2221 agaggaagcg gtcgacatac ttcgcggcca gcccctggta catgacctgg ccgtcatggg
     2281 cggccagctc gatggcgagc tccgccatgt tctggcagaa gaacgccatc caggccgtgc
     2341 cgtcggcctg ctccaggtag ccgccggtcg gcagcggggc gctgcggtcg aacacgctga
     2401 tgttgtcgag cccgaggaag ccgccgtcga acaggttctt gcccgagcgg tccttgcgat
     2461 tggcccacca ggcgaaattc aacgacagct tgtgaaacgc ggctgccagg aactccgtgt
     2521 ccagcgatcc gccccgggcc tggtagattc gggtcaggaa gagcgatgcc caggcgtgga
     2581 ccggcggatt cacgtcgccg aaagcccact cgtaggccgg gatctggccg ttcgggtgca
     2641 ggtaattggc gttgagcagc aggtcgagct gcttcttggc aaagtccggg tccacgacgg
     2701 ccagcgcgac ggtgtggaac gcgaggtccc acgcggcgta ccagggatac tcccacttgt
     2761 ccggcatcga gatgatgtcg tcgttgacca tgtggaccca ttcctggttc cgatagtagc
     2821 ggctccccgg ccg
//
LOCUS       DASUGJ010000282         3155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1987867, whole genome shotgun sequence.
ACCESSION   DASUGJ010000282 DASUGJ010000000
VERSION     DASUGJ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1987867"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2312)
                     /locus_tag="VFV93_06295"
     CDS             complement(<1..2312)
                     /locus_tag="VFV93_06295"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5164988.1"
                     /translation="MMTELLATKLRIPPPTQHVVRRTRLVDALDAAIPRHKLTLISAP
                     AGYGKTTLLADWARASRLPVAWLTLGEEDNDLARFFRYLFTAWERIEPGVADSALGVL
                     LGAMEPEPDAVLTAFVNLASELDDPTVFVLDDYQVIEQAAIHVALTFLLDHLPPSTHV
                     VLACRDEPPLPLARYRARQELVELDAGDLRFSVAETDLFLNRQLGLDLADDTIASLHA
                     QLEGWIAGTQMAALTLRRHDQPPEDLIVSGRHRFIADYLSQDVLAHLPGEQRRFLLQT
                     SILDRLSGPLADAVSGGSDGQTMLETLERENLFLVPLDDNREWYRYHRLFADFLREEL
                     RRQDPDEIRDLHRKAARWYFAHDLAEQAFQHALASQDADLVFEVLDRYVLIKLHGGDL
                     KGLETWLDAIPAAWYDEHPQFGLARATLLAGFGAFEACLQSIKEVEALLTPIDTQEAR
                     WQMGRVMAFHCFVACVQNDLDRAERLAEDARQTLRDDDVGFRASIFHALGDTYSRNGR
                     WQEAHAAYLGVLAHGRHYAARMQAAHVYGALADLELRQGHLQVAAGYWRKALAAINEP
                     SNWGRLPLPVIGWVYIRMGELLYEWNQPLDAWEHVMRGLERAEAGGDVRSMLAGYLLA
                     GRIRLTAGDTETAANYLELARPLVEDAPIPEWTSRFERLQLELWLAQDRLRAAVDWSD
                     AMLAGDTLRDRPEPEVARLTVARALIVKGDSRSVDMALALLAPLIRTAEAAGRTGIRI
                     EALALQALATWQRGDRPGALTLIEHALRLA"
     gene            complement(2382..>3155)
                     /locus_tag="VFV93_06300"
     CDS             complement(2382..>3155)
                     /locus_tag="VFV93_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5164989.1"
                     /translation="GGYYTDMVEIVGGLDLLPRAFYPALRERIRFGARMVAVEQSPEA
                     VTVHFQTGGGRRSITGDAAILTVPFPVLRHVEVLPELSRGKQRAIRQLHYDASAKILL
                     QFRRRFWEEVDGIYGGGSFSDLPIRVTYYPDHGAEMGRGVVLASYTWSEDAQRWGSLS
                     PADRISQALENLARIHPEAIESFEVGASKMWHDDEFAGGAFALFEPGQQTLLHSLIVQ
                     REGRIHFAGEHTSLHHAWIQGAIESGLRVARDIHQPARA"
BASE COUNT          508 a         1032 c         1101 g          514 t
ORIGIN      
        1 tctgcgaggc ggagcgcgtg ttcgatcagc gtgagcgcgc cgggccggtc gccacgttgc
       61 caggttgcca gtgcctggag cgccagcgcc tcgatccgga taccggttcg ccccgctgct
      121 tcggccgtcc ggatgagcgg cgcgagcaac gccagcgcca tgtcgacgga tcgtgagtcg
      181 cccttgacga tcagcgcgcg ggcgacggtc agtcgggcga cttccggctc aggtcgatcg
      241 cggagcgtgt cgccggccag catcgcgtcg gaccagtcga cggctgcccg gagccgatcc
      301 tgcgccagcc agagttccag ttgtagacgc tcgaagcggc tggtccactc aggaatcggg
      361 gcgtcctcga ccagcggacg cgccagctcc agatagttgg cggctgtctc agtgtcgccg
      421 gcggtcagcc ggatgcggcc ggcgagcagg tagccggcga gcatcgagcg aacatccccg
      481 cccgcctcgg cgcgctccag cccgcgcatg acgtgctccc aggcgtccag cggctggttc
      541 cattcgtaga gcaactcgcc catccgaatg tagacccagc cgatgaccgg cagcggtagc
      601 cggccccagt tggacggctc attgatcgct gccagcgcct tgcgccagta gccggccgcc
      661 acctggaggt gtccctgtcg aagctccaga tcggccagcg cgccgtagac gtgggccgcc
      721 tgcatccgtg cggcatagtg gcgaccgtgg gccagcacac ccaggtaggc ggcgtgcgcc
      781 tcctgccagc ggccgttgcg gctgtaggtg tcgccgaggg catggaagat cgatgcgcgg
      841 aagccgacat cgtcgtcgcg gagtgtctgt cgcgcatctt cggccagtcg ctcggcccgg
      901 tcgaggtcgt tctggacgca ggcgacgaag cagtggaaag ccatgacgcg ccccatctgc
      961 cagcgcgctt cctgggtgtc aatcggcgtc aggagcgctt cgacttcctt gatcgattgg
     1021 agacacgcct caaacgcgcc gaatccggcc agcagggtcg cccgtgcgag accaaattgc
     1081 gggtgttcgt cgtaccaggc cgccggaatc gcatccagcc acgtctcaag tcctttgagg
     1141 tctccgccgt gcagcttgat gaggacatac cggtcgagga cctcgaagac gaggtcggcg
     1201 tcctgacttg ccagcgcatg ttgaaacgcc tgctcagcca gatcgtgggc gaaataccat
     1261 cgcgctgcct tgcggtggag atccctgatc tcgtccgggt cctgtctccg cagctcctcg
     1321 cgcaggaaat cggcgaacag ccggtggtag cggtaccact cgcggttgtc atccagcggg
     1381 acgaggaaca gattctcgcg ttccagcgtc tccagcatcg tctgcccgtc cgatccgccg
     1441 gagacggcat cagccagcgg gccggacagg cggtcgagga tgctggtctg gagcaggaac
     1501 cggcgttgct cgccgggcag gtgggccagc acgtcctgac tcaggtagtc ggcgatgaag
     1561 cggtgccggc cgctgacgat cagatcctcc ggtggctggt cgtgacgccg cagggtgagt
     1621 gccgccatct gtgtcccagc gatccagccc tccagttgcg cgtgcagcga ggcgatggtg
     1681 tcgtcggcca gatcgagccc tagctggcga ttgaggaaca gatcggtttc tgcgacgctg
     1741 aagcgcaggt cgccggcgtc gagctcgacc aactcctgac gtgcccggta gcgggccagc
     1801 ggcagtggcg gctcgtcgcg gcaggccagg acgacgtggg ttgacggcgg tagatgatcg
     1861 agcaggaagg tcagcgccac gtggatggcg gcctgctcga tcacctgata gtcgtcgagg
     1921 acgaacaccg tcggatcatc cagctcgctg gcgagattga cgaaggcagt cagaacggcg
     1981 tcaggctccg gctccatcgc gccgagcagg acgccgagtg cgctgtcggc gacccccggc
     2041 tcgatacgct cccaagcggt gaacaggtag cggaagaatc gagcgaggtc gttgtcctct
     2101 tccccaagcg tcagccaggc gaccggcagg cggctcgctc gtgcccaatc ggccagcagt
     2161 gtcgtcttgc cgtaaccggc tggggccgag atcagcgtca gcttgtggcg cgggatggcg
     2221 gcgtcgaggg catcgaccag ccgggtacgg cgcacgacgt gttgcgtcgg agggggtatc
     2281 cgcagcttgg ttgccaggag ttcggtcatc atccggccac cgccatctca ggttgtggct
     2341 tcacatgtat ccactatacg cgccggggta aagtcccacc ctcacgcgcg tgccggctgg
     2401 tgaatatccc gcgcgacgcg caggccggac tcgattgcgc cctggatcca ggcgtggtgg
     2461 agcgaggtgt gctcgccggc gaagtggatc cggccctcgc gctggacgat caggctgtgc
     2521 aacagcgtct gctggcccgg ctcgaagagc gcgaaggcgc cgccggcgaa ctcgtcgtcg
     2581 tgccacatct tcgacgcccc gacctcgaag gattcgatcg cctccgggtg gatccgggcg
     2641 aggttctcca gtgcctggct gatccggtcg gccggcgaga gcgagcccca acgctgggcg
     2701 tcctccgacc aggtgtagct ggcgaggacg acgccgcggc ccatctcggc gccgtggtcg
     2761 gggtagtagg tgacgcggat cggcagatcg ctgaagctac cgccaccgta gatgccgtcg
     2821 acctcttccc agaagcgacg gcggaactgg agcaggatct tggctgaggc gtcgtagtgc
     2881 agctggcgga tggcgcgctg cttgccgcgc gagagctccg gcaacacctc gacgtggcgc
     2941 agcaccggga agggcacggt caggatcgcc gcatcgccgg tgatcgaccg ccggccgcct
     3001 cctgtctgga agtgcaccgt caccgcctcg ggcgactgct ccaccgccac catgcgcgcg
     3061 ccgaaacgga tgcgttcgcg caacgccggg tagaaggcgc gcgggaggag gtccagcccg
     3121 ccgacgatct cgaccatgtc ggtgtagtag ccccc
//
LOCUS       DASUGJ010000283         5566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1848118, whole genome shotgun sequence.
ACCESSION   DASUGJ010000283 DASUGJ010000000
VERSION     DASUGJ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848118"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VFV93_06305"
     CDS             <1..897
                     /locus_tag="VFV93_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5164990.1"
                     /translation="RPEVKHRLGLAPDDLWPLNPRLVYGSISGFGQDGPYASRPGFDQ
                     IAQGMGGLMSITGEPGRGPLRVGIPIADLCAGMFCAQGVLLALLEREVSGKGQWVTSS
                     LLEAQIQMLDFQAARYLKDGEVPVSAGNDHPTSIPTGVFPTSDGHINIAASGEAIYQR
                     FCEAISAPHLITDERFKSATLRSHNRAEMNAAISAITCQEPSQYWIDLLNEAGCPCGP
                     IYSMDQVFADAQVRHLGMAVPVEHPRLGMFEVVNQAIKMSRTPSAIRTATPEQGEHTD
                     EVLAELGYDDERIRQFRTNGVV"
     gene            916..1719
                     /locus_tag="VFV93_06310"
     CDS             916..1719
                     /locus_tag="VFV93_06310"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX5164991.1"
                     /translation="MKQLELSTERMLAHVEDGIGWITFNQPEKRHAVSYDMWLAIPEI
                     IASFTANDDVRVVVMKGAGDRAFVSGADISEFEDKRGSADAVTTYGEASGAANAALRS
                     LEKPLIAMIRGYCIGGGLGIALAADLRISAEDGQFAIPAAKLGLGYGYGGIKELVNIV
                     GPSFAKEIFFTARRFTAQEAKDMGLVNRVVPVADLERTTTELAGLIADNAPLTVKAAR
                     AAINEATKDPAQRDIAKVDAMVAQCFDSQDYIEGRRAFMEKRKPQFAGR"
     gene            2133..2537
                     /locus_tag="VFV93_06315"
     CDS             2133..2537
                     /locus_tag="VFV93_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5164992.1"
                     /translation="MFRDPQINYYVRDVDASVRFYCDLFGFEETFRTPDSGTPEHVEL
                     RLGGLILGLASTESARRTHGLTTGGGNPRAEVCLWADDVDRSYADLVARGVPSLSAPH
                     NFLGGRLRAAWVADPDGNPVEIVAEQQVVSSP"
     gene            2575..3267
                     /locus_tag="VFV93_06320"
     CDS             2575..3267
                     /locus_tag="VFV93_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX5164993.1"
                     /translation="MQTEDDVPQLTPEQLTALRRIDSPTIANAIETFEVRPRVSGYVG
                     YDIRCIFPDLPPMVGYAVTCTVDSTTEGRVGIGFQRLYEILETAPKPAVVVMQDVGQD
                     RLHSCHAGEVMSTTMKRLGAVGILTDGGLRDVREVAALGGFHYFCAGLVVSHGNPLCI
                     SVNDEVTISGMVVRPGDLLHGDQNGVVLIPDQCAAEVADAAHRIWAREDQTLRQIAEP
                     GYTVRGKDELKH"
     gene            complement(3352..>5566)
                     /locus_tag="VFV93_06325"
     CDS             complement(3352..>5566)
                     /locus_tag="VFV93_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5164994.1"
                     /translation="SLVSYIAISNRGGIEAGVEHTDAVVAAANEALEGSPYRALPFKR
                     NGLDDAEVQGDMFLQMFLIFGLFSVAVGVLLIFLIFVMLAAERKPEMGMARAVGLKRR
                     QVTEMFLAEGIAYDLGAALVGAVLGIGVAFLMIQVLAGIFGDQLEIRPYASWRSLLIS
                     YTLGVVVTFVTILVSSWKVSKINITAAIRDVAEPVHKRESRRWLVFGVVGLVVGVLGI
                     WSGASSGNAFALSMGISLVPLSLAAVVRRFGVPARPVFSVAAAIVLVYWLLPASVSDR
                     FLPETEGGIEMFFLSGIMMVASSTVLIIWNADLITDGMGQLGRVFSRWLPAVKTAIAY
                     PLANRVRTGMTVAMFSLVVFSLVMMAAIMINVEEVFSSEDAGGGWAIQAVQAPTNPIT
                     DFRQALIDNNVDDSAVVETSRVVSMPIFRTQVREPDADRWSTYTINGVDANFFDHSQI
                     TFQTRATGYDSDAAIMAALRADPSLAIVDSFVLPSVGFGADENQLQFSDISTGDTSMP
                     PMPVEIADPATGQTRTVTVIGIMDSRMFTLFGIFLSESTFTDVYEQPEAITYYVQLAP
                     GTDAKALAASMESGLIGYGVQAQSIREIVDEQMSAQTSFLRLFEGFMGLGLVVGIAAV
                     GVIAFRSVVERRQQIGMLRAIGYQRGMVAVSFMLESLLVTLLGVISGTILGLLLAWNL
                     VTSDYFLDVGRSRFIVPWLDVVMFIVVALVAAVLMAYVPARRAARVPIAQALRYE"
BASE COUNT         1081 a         1808 c         1805 g          872 t
ORIGIN      
        1 cgacccgagg tgaagcatcg cctgggcctg gcgcccgacg acctgtggcc gctcaacccc
       61 cgtctggtct acggcagcat ctccggcttc ggccaggacg gcccgtacgc gtcccggcct
      121 gggttcgacc agattgccca gggcatgggc gggctcatgt ccatcaccgg agagccgggc
      181 cgcgggccgc tgcgcgtcgg aatcccgatt gccgacctct gcgccgggat gttttgcgca
      241 cagggcgtcc tgctggccct gcttgagcgg gaagtctccg gcaaagggca gtgggtcacg
      301 tcgtcgctgc ttgaagccca gatccagatg ctcgacttcc aggctgcgcg ctacctgaag
      361 gatggcgagg tgccggtcag cgccggcaac gaccatccga cgagcatccc gaccggtgtg
      421 ttcccgacgt cggacggcca catcaacatc gccgcatccg gtgaagcgat ctaccagcgc
      481 ttctgtgagg cgatcagcgc tccccatctg atcactgatg agcgtttcaa gtcggcgaca
      541 ctgcgttcgc acaaccgggc cgagatgaac gcggcaatct cggcgatcac ctgccaggaa
      601 ccaagccagt attggatcga cctcctgaat gaggcgggtt gcccgtgcgg gccgatctac
      661 tcgatggatc aggtgtttgc agacgctcag gtgcggcatc ttgggatggc tgtcccggtt
      721 gaacacccgc ggcttggcat gtttgaggtc gtcaaccagg cgatcaagat gagccgcacc
      781 ccgtccgcta tccgcaccgc aacacctgag cagggcgaac acacggacga ggttctcgct
      841 gaactggggt atgacgacga gcgcatccgg caattccgca ccaacggcgt cgtctgagga
      901 cgcaggagca agaagatgaa gcaactcgag ctatcgaccg agcgcatgct ggcgcatgtc
      961 gaggacggca ttggctggat cacgttcaac cagccggaga agcgccacgc cgtgtcatat
     1021 gacatgtggc tggcgatacc ggagatcatc gcctcgttta ctgccaacga tgacgtccgt
     1081 gtggtggtca tgaaaggcgc cggcgaccgg gcgttcgtct ccggagccga tatttcggag
     1141 ttcgaggaca agcgcggctc tgccgacgcc gtgacgacgt acggagaggc gagcggagcc
     1201 gcgaacgcgg cgctcaggtc gctggaaaag ccgttgatcg cgatgatccg cggctactgc
     1261 atcggcgggg ggctcggcat cgcactcgct gcagacctgc gcatttccgc agaggacggc
     1321 cagttcgcca tcccggccgc aaagctcggc ctcggctacg gatacggcgg catcaaggag
     1381 ctggtgaaca tcgtcggccc gtcgtttgcc aaagagatct tcttcaccgc acgacggttc
     1441 actgcgcagg aagcgaagga catgggactg gtgaaccgcg tggtgccggt cgccgacctt
     1501 gagcgcacga caacggagct tgcgggactg atcgcggaca acgcgccgct gaccgtgaag
     1561 gcggcaagag cagccatcaa cgaagcaacc aaggacccgg cacagcgcga tatcgcgaaa
     1621 gtggacgcga tggtcgcgca gtgcttcgac agccaagact acatcgaagg ccgccgtgcg
     1681 ttcatggaaa aacgcaaacc acagtttgca ggtcggtaag gacgaacggt tacaccggcg
     1741 acgcaatgcc tgcgaacatg ctgagcgagt accgtccggc ttccgcaacg gctcggtgaa
     1801 ctccgtggtg attgatccac agagcggcac gctgggcagc gtcaacaatg gcgcctgcca
     1861 tgccggccac gcgcaggacg aggagtgggg cttcgcctgg agcacaccgc gcccggtgga
     1921 ggacgtcgag accgggcggg agcggtacgc cagtcgcgca ctggtgggcc ggcagccgtc
     1981 tgatgtggta cgagccgtac tggtcggacg cgctcacgcg actccgcgac ctggcggaag
     2041 gacacgaacc gcccgaatag gtgcaaccac cgtgttcgtg tcgcgtaggg gcgatggact
     2101 catgagtgag acacaataga tggaggatac cgttgttccg cgatccgcag atcaactact
     2161 acgtccgcga tgtcgacgcg tcggtccgct tctactgcga tctgttcggc ttcgaagaga
     2221 cgtttcgcac ccccgactcc ggcacgcccg agcatgtcga gctgcggctc ggcgggctga
     2281 tcctcggact ggcgtcgacc gagtcggctc ggcggacgca tggcctgacg accggcggcg
     2341 gtaacccgcg agctgaagtc tgcctgtggg ccgacgatgt cgatcgatcc tacgccgatc
     2401 tcgtcgctcg cggcgtcccc tcgctgagcg ccccgcacaa cttcctcggc ggtcgcttgc
     2461 gggcggcctg ggtcgccgac ccggacggta atccggtcga gatcgtcgcc gagcagcagg
     2521 tggtatcttc cccataggct ccgactgacc ggtggagccg gcgaccgata cgacatgcag
     2581 acggaggacg acgtgccgca actgacgcct gagcaactga ccgcgctccg acggatcgac
     2641 agcccgacga tcgccaacgc gattgagacg ttcgaagtgc gtccacgggt cagcggctac
     2701 gtcggctacg acatccgctg catcttcccg gacctgccgc cgatggtcgg ctacgccgtg
     2761 acctgcaccg tcgacagcac gaccgaaggc cgggtcggga tcggcttcca gcgactctac
     2821 gagatcctgg agacggcgcc caagccggcc gtcgtcgtga tgcaggatgt cgggcaggac
     2881 cggctgcaca gttgccacgc cggtgaggtg atgagcacga cgatgaagcg gctcggcgcg
     2941 gttgggattc tgaccgacgg cggcctgcgc gacgtccgcg aggttgcggc gctgggaggc
     3001 ttccactact tctgcgccgg gctggtcgtc tcgcacggca acccgctctg catcagcgtc
     3061 aacgacgagg tcacgatctc cgggatggtc gtccgccccg gagatctgct gcacggcgac
     3121 cagaacggcg tcgtcctgat ccccgaccag tgcgccgccg aggtcgccga cgccgcccac
     3181 cgcatctggg ccagggagga ccagaccctg cgccagatcg cggaacccgg ttacaccgtt
     3241 cgcgggaaag acgaactcaa gcactgacct gcctcgccat cggtaacctg accgatgcga
     3301 acggcgccgt ctgcagacgg cgccgttggc ccagggccgg ggtacccgcg cctactcgta
     3361 gcgtagagcc tgggcgatcg gtaccctggc tgcccgtctg gccgggacgt aggccatcag
     3421 caccgccgct acaagcgcga cgacgatgaa catcaccacg tcgagccacg gcacgatgaa
     3481 ccgacttcgg ccgacatcga ggaagtagtc gctcgtgact agattccagg ccagcagcag
     3541 gccgaggatg gtacctgaga tgacacccag tagcgtcacc agcagcgact ccagcatgaa
     3601 gctgacggcg accatcccac gctggtagcc gatcgcccgc aacatgccga tctgttgccg
     3661 gcgctcgaca accgagcgga atgcgatcac gccgacggcc gcgatgccaa cgaccagacc
     3721 cagccccatg aacccctcga agaggcgcag gaagctggtc tgggcgctca tctgctcgtc
     3781 aacgatctcg cggatcgact gggcctggac gccgtagccg atcaggcctg actccatcga
     3841 ggccgccagc gccttcgcgt cggtccccgg cgcgagctgc acgtagtagg tgatcgcctc
     3901 tggctgctcg taaacatcgg tgaaggtcga ctcggacagg aagatcccga acaacgtaaa
     3961 catccgcgag tccatgatgc cgatcaccgt caccgtccgc gtctggcctg tcgcgggatc
     4021 ggcgatctcg accggcatag gcggcatcga ggtatcgccc gtggagatgt cactgaactg
     4081 gagttggttc tcgtcggcgc cgaagccgac cgacggcagc acgaacgagt cgacgatggc
     4141 cagcgaggga tcggcccgca gcgcggccat gatcgccgca tcggagtcgt aaccggtcgc
     4201 tcgcgtctgg aacgtgatct ggctgtggtc gaagaagttc gcgtcaacgc cgttgatcgt
     4261 gtacgtgctc catctgtcgg cgtcgggttc gcgaacctgg gtacggaata tcggcatcga
     4321 gacgacccgc gaggtctcga cgaccgcgct gtcatcgaca ttgttatcga tcagcgcctg
     4381 gcgaaagtcg gtgatcgggt tggtcggcgc ctggacggcc tggatcgccc atccgccacc
     4441 agcgtcctca ctggagaaga cctcctcgac gttgatcatg atggcggcca tcatcaccag
     4501 cgagaagacg accaggctga acatcgcgac cgtcattccc gttcggaccc ggttggcaag
     4561 cgggtaggcg atcgccgtct tgacggccgg cagccagcgg ctgaagaccc ggccgagctg
     4621 gcccatcccg tcggtgatca ggtcggcgtt ccagatgatc agcaccgtcg acgacgcgac
     4681 catcatgatg cccgacagga agaacatctc gatcccaccc tcggtctcag gcaggaatcg
     4741 gtcggagacc gaggccggca ggagccagta gacgaggacg atcgccgccg cgaccgagaa
     4801 gacgggccgg gccggaacgc cgaatcgtcg cacgaccgcc gccagactga gcggcacgag
     4861 cgagatgccc atcgacaggg cgaaggcgtt gccggacgac gcaccggacc agatccccag
     4921 cacgccaacc acgaggccga cgacgccgaa gaccagccag cgccggctct cccgcttgtg
     4981 aaccggttcg gcgacatcgc ggatcgcggc cgtgatgttg atcttactga ctttccacga
     5041 cgagacgagg atcgtcacga aggtgacgac gacgccaagt gtgtaggaga tcagcaggct
     5101 gcgccacgac gcgtaggggc ggatctcaag ctggtcgcca aaaattccgg ccaacacctg
     5161 gatcatcagg aaggcgacac cgataccgag gacggccccg accagcgccg ccccgaggtc
     5221 gtaggcaatc ccctcggcga gaaacatctc cgtcacttga cggcgcttga ggccgacggc
     5281 ccgcgccatc cccatctcgg gcttgcgctc ggcagccagc atgacgaaga tcaggaagat
     5341 cagcagcaca ccgacagcca ccgagaagag cccaaagatc aggaacatct gcaggaacat
     5401 gtcgccctgc acttcggcgt catcgagacc gttgcgcttg aacggcagcg ctcggtaggg
     5461 agatccctcc agcgcctcgt tggcggctgc aacgactgcg tcggtatgct cgacgcctgc
     5521 ttcgatcccg ccccggttcg atatcgcgat gtaactcacg agcgag
//
LOCUS       DASUGJ010000284         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1848397, whole genome shotgun sequence.
ACCESSION   DASUGJ010000284 DASUGJ010000000
VERSION     DASUGJ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848397"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1946)
                     /locus_tag="VFV93_06330"
     CDS             complement(78..1946)
                     /locus_tag="VFV93_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5164995.1"
                     /translation="MVPIDKEQIRLLEQRLRGAGVDRRTFLKIAGAAMAAPAAGSLLA
                     ACGGDNDDDDTPSATSTAAGTGGESAATATTGETGSEASPSTDSGAATAATGETGAGV
                     PDAVQFLNDTLQNGDPASHDFNADLYCNGVPQIWAGLLSFDQDFNPSPDWAESWEPNE
                     DATMWTFHIRPDNTGFSNGDPVTAETFAYSWKRMLDPDTLAPYASILFDIKGAEDINL
                     NGADPDTLGVRVVDDWTLEVDMIAPRGMFPTIVAYYACVPTHPESAEKGGNFTDPGEI
                     GGPVISNGPFVLTEWEHSQRCTVEKNPNYWNASAITLEKINNLIVPTEQGILPYEAGE
                     VDWIVVPGSELPRVQADPTMSAEVQKWVEPLIWKLLPQVTVAPFDDIKVRRALSHSID
                     RDRINELTNGGGDPAHCLVPPGLFGYFGSDFADIMAFDPEKALAELEGTKYEGGENWP
                     KVTMILRNEAALNSNIMAEDLAAQIQESIGMEIEIQVMDFQAFRDLMFTNTSQLVFVR
                     WYYDYPDPNNGYFDMFYSNKESGKRQAWSNKEFDELVVKGKEAADPDERLEYYRQAEQ
                     IMQDEVAYIPIVYRNAYDVYKPWVKGVPVNMQGFALPNGNIYVGMWNTVYTEGRPA"
     gene            complement(2203..>2484)
                     /locus_tag="VFV93_06335"
     CDS             complement(2203..>2484)
                     /locus_tag="VFV93_06335"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5164996.1"
                     /translation="AGTMSVLAIFVGYILWYRGLRRLQPSQVAVYVYLVPFFGVLSAW
                     LLLGETITAWLLLGGATILTGVVVTNSGRRPAAEPLPEGGTASHNPQAL"
BASE COUNT          385 a          770 c          854 g          475 t
ORIGIN      
        1 gtaagccctc accccccgac tccctctccc aaagctggga gagggggtcg gggggtgagg
       61 gcagcgggtg agggccgcta cgccggtcgc ccctcggtat agaccgtgtt ccacatgccg
      121 acgtagatgt tgccgttcgg gagggcgaag ccctgcatgt tgaccggcac acccttgacc
      181 cacggcttgt agacgtcgta ggcgttgcgg tagacgatcg ggatgtaggc gacttcgtcc
      241 tgcatgatct gctcggcctg gcggtagtac tccagccgct cgtcggggtc ggccgcttcc
      301 ttgcccttga cgaccagctc gtcgaactcc ttgttcgacc acgcctggcg cttgccggac
      361 tccttgttgg agtagaacat gtcgaagtag ccattgttcg ggtccggata gtcgtagtac
      421 cagcggacga agaccagctg cgaggtgttg gtgaacatca ggtcgcggaa ggcctggaag
      481 tccatcacct ggatctcgat ctccattccg atcgattcct ggatctgggc agcgaggtcc
      541 tcggccatga tgttggagtt gagggccgcc tcgttacgca ggatcatcgt gaccttgggc
      601 cagttttcgc cgccctcgta cttggtgcct tccagctcgg ccagcgcctt ctcgggatcg
      661 aaggccatga tgtcggcgaa gtcgctgccg aaatagccga acaggcccgg cgggacgagg
      721 cagtgggccg ggtcgccgcc gccgttggtc agctcgttga tgcggtcacg gtcgatcgag
      781 tgcgacagcg cccggcggac cttgatgtca tcgaacggcg cgaccgtcac ctgcggcagc
      841 agcttccaga tcagcggctc gacccacttc tggacctcgg cgctcatggt cgggtcggcc
      901 tggacgcgcg gcagctctga gccgggaacg acgatccagt cgacctcgcc ggcctcgtag
      961 ggcaggatgc cctgctcggt cgggacgatc aggttgttga tcttctccag cgtgatcgcg
     1021 ctggcgttcc agtagttcgg gttcttctcg accgtgcagc gctggctgtg ctcccactcg
     1081 gtcagcacga acggcccgtt ggagatgacc ggcccgccga tctcgccggg gtcggtgaag
     1141 ttgccgccct tttcggccga ctcggggtgg gtcgggacgc aggcgtagta ggcgacgatc
     1201 gtcgggaaca tgccgcgcgg agcgatcatg tcgacttcga gcgtccagtc atcgacgacc
     1261 cggacaccga gggtatcggg atcggcgccg ttgaggttga tgtcctcggc gcctttgata
     1321 tcgaacagga tcgaggcgta cggcgccagc gtatccggat cgagcatccg cttccaggag
     1381 taggcgaatg tctcggccgt gaccgggtca ccattcgaga agccggtgtt gtcggggcgg
     1441 atgtggaagg tccacatcgt cgcgtcctcg ttcggctccc acgactcggc ccagtctggg
     1501 ctggggttga agtcctggtc gaagctgagt agtccggccc agatctgcgg gacaccgttg
     1561 cagtacaggt cggcgttgaa gtcgtggctg gccgggtcgc cgttctgaag cgtgtcgttg
     1621 aggaactgaa cggcgtccgg gacgccggcg cctgtttcgc cggtcgccgc cgttgctgcg
     1681 ccgctgtcgg ttgatggcga cgcctcgcta ccggtctcgc cggtcgtcgc ggtcgcggca
     1741 ctctcgccgc cggtgccggc cgctgtgctg gtcgcggacg gcgtgtcgtc atcatcgttg
     1801 tcgccaccgc aggccgccag tagcgagcct gccgccgggg ccgccatcgc cgcgccggcg
     1861 atcttcagga acgtgcggcg atcgacgcct gcgccgcgca gccgctgttc gagcagacgg
     1921 atctgctcct tgtcaatcgg aaccactcga tctcctcctg gacatttcgc gcagccctgg
     1981 ccagggccgc gtcgtctctc atgccggccc gacggacgcc gggccggctg tctactgaac
     2041 ccaaagggca atatatcaga ttccacatat gtgcaagcct gaattatggt gagttgagcg
     2101 tgtggttgcg acccattcgg cgtgatgcaa cgcgtcacgc gaaccaatcg gcgccgcgag
     2161 acgcattgac gctggaaagg gttggttgat cgcgttgcgg cactacaacg cctgtggatt
     2221 gtgggacgct gtgccgccct ctggcaacgg ctccgccgct gggcggcggc cggagttggt
     2281 gacgacgacg ccggtcagga tcgttgcgcc gccgagcagg agccaggcgg tgatcgtctc
     2341 gcccagcagc agccaggccg agaggacgcc gaagaacggg acgaggtaga cgtagaccgc
     2401 cacctggctg ggttggaggc ggcgcaggcc gcggtaccag aggatgtagc cgacgaagat
     2461 cgccaggacg ctcatcgttc cggc
//
LOCUS       DASUGJ010000285         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1710619, whole genome shotgun sequence.
ACCESSION   DASUGJ010000285 DASUGJ010000000
VERSION     DASUGJ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710619"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFV93_06340"
     CDS             complement(<1..307)
                     /locus_tag="VFV93_06340"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HEX5164997.1"
                     /translation="MATIDNTATQAPQVDGPSEVTPDILFQIAGGFMTAKHLFIANEV
                     GLFEHLASGPASLYDLAQRTGIARRRLRIVADAMVALGLLECQDGQYQNGPIAATFLI
                     "
     gene            complement(475..>2294)
                     /locus_tag="VFV93_06345"
     CDS             complement(475..>2294)
                     /locus_tag="VFV93_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5164998.1"
                     /translation="ICARLDGLPLAIELAAARIKLLPPAALLARLDHRLQLLTGGPRD
                     LPARQQTLRNTLDWSYRLLNEDEQRLLRCLAVFSGGCTLDAAEAVYIMPGDSSQDAGP
                     IDVLDGVAALIDNSFVRQVEQGGNEPRLMLLETIREYGLERLSASGELEIVARQHAGY
                     FLALAETAEPKLMGAEQGDWLNRLAIERDNLRAALRWALEHGETETALRLGGALWRFW
                     FMHGDLSEGRRWLDEGLAGGAVAPAVRAKALSGSGVLAHYQGDLNRAAALCGESLALS
                     RQIGDTVGIADALNGLALVPRSGGNYAAARAMYTESLALLRESGDRWRVAYTLIYLGV
                     IRFVQHDVVGGRHSVEEGLALFRGVGDRWGIARALNILGEMALAAGDASAALALMTEA
                     QARYGNIGDRLGATRSLSSLGDVALVQNDLPVARRCYEQALVLLAEVGDRMMVAHCLA
                     GLATVVASGTDVARAARLSGAAAALLDAAGGIEHSVTPVNVARGLEIARAALSEEAFA
                     TAWAEGQAMSLEQAIACALEPERTEPSASLPDPGVRLSKREIEVVRLLVEGKSNQQIA
                     EALSISYHTAGNHVAHILNKLNLESRTAVAAWAVRHGIG"
BASE COUNT          366 a          806 c          757 g          365 t
ORIGIN      
        1 cgatcaggaa ggtcgccgcg atcgggccgt tctgatactg accgtcctgg cactcaagca
       61 ggccgagggc caccatggcg tcggccacga tgcgcaaccg gcggcgggcg atccccgtgc
      121 gttgcgcgag gtcatagagg ctggccggcc cgctggccag gtgctcgaac aggccgactt
      181 cattggcgat gaacagatgc ttggcggtca tgaagccgcc ggcgatctgg aagaggatat
      241 cgggggtcac ctcgctgggc ccatcgacct gtggcgcctg agtagctgtg ttgtcgattg
      301 ttgccattgg gagctccttg ccagtgaccg atttgcgtcg gtgagcgcat ggatgcggcc
      361 accccggcca tcatatggac agtatgggct gcacgctggg agcacgcatc gtgcacattc
      421 gtcatccagg ctccaacggg acatgacgaa tcttggctat agaacggcga ggcactagcc
      481 gatcccgtgt cgcacggccc aggcagctac cgctgttcgc gactcgaggt tcagcttgtt
      541 caggatgtgg gcgacatgat tgccggccgt gtggtagctg atcgacaacg cctcggcgat
      601 ctgctggttg gacttgccct cgacgagcag ccgcacgacc tcgatctcgc gcttcgacag
      661 gcgaacgccg ggatcgggta gcgacgcaga tggttcggtt cgctccggct ccagcgcaca
      721 ggcgatcgcc tgctccagcg acatcgcctg cccctcagcc caggctgtgg cgaacgcctc
      781 ctcgctgagc gcggcgcgtg cgatctccag cccgcgtgcg acgttgaccg gggtgacaga
      841 gtgctcgatg cctccggccg cgtctagcag tgccgcggcc gccccggaca gccgcgcggc
      901 gcgggcgacg tccgtgccgg atgcgacgac cgtcgccaga ccggccagac agtgcgccac
      961 catcatccga tcgcccacct cggccagcag caccagggcc tgctcatagc agcggcgcgc
     1021 cactggcaga tcgttctgaa cgagcgcgac gtcgcccagc gaggagagcg agcgggtagc
     1081 gccgagccgg tcgccgatat tcccgtagcg tgcttgcgcc tccgtcatca gcgccagcgc
     1141 ggcgctggcg tcgccggcag cgagcgccat ttctcccagg atgttgagcg ccctggcaat
     1201 cccccaccgg tcgccgactc ctcggaacag cgccaagccc tcctcgacgg agtggcgtcc
     1261 gccgacaaca tcatgctgga cgaagcggat caccccgagg tagataaggg tgtaggcgac
     1321 gcgccagcga tcgcccgact cgcgcagcaa cgccagactc tccgtgtaca ttgcgcgcgc
     1381 cgcagcgtag ttgcctccgg aacgagggac cagggccagg ccgttcagcg cgtcggcgat
     1441 tccgaccgtg tctcctatct gccgggacag cgccaggctc tcgccgcaca gtgctgccgc
     1501 gcggttcagg tcgccctggt agtgcgcgag cacgccacta ccgctgagcg ccttcgcccg
     1561 gacggccggc gcgacggcac caccagccag tccctcgtcc agccagcggc gaccttcgct
     1621 caagtcgccg tgcatgaacc agaagcgcca gagcgcccca ccgaggcgca gcgccgtctc
     1681 tgtctctcca tgctccagcg cccagcgcag cgccgcccgc aggttgtcgc gctcgatcgc
     1741 gagccggttc agccagtcgc cctgttcagc gcccatcagc ttcggctctg ccgtctcggc
     1801 cagcgccagg aagtagcctg cgtgctgtcg cgcgacgatt tccagctccc cgctggccga
     1861 cagacgctcc agcccgtact cgcggatcgt ctccagcaac atgagtcgtg gctcattgcc
     1921 gccctgctcc acctggcgca cgaagctgtt gtcgatgagc gccgcgacac catccagcac
     1981 gtcgatcggc ccggcgtcct gggaagagtc gccgggcatg atgtagaccg cctcggccgc
     2041 gtcgagcgtg cagccgccgc tgaagacggc caggcagcgg agcagccgtt gctcgtcctc
     2101 attgaggagg cgatagctcc agtcgagcgt gttgcgcagc gtctgctgcc gagcggggag
     2161 gtcgcgcggc ccgcccgtca gcagctggag ccggtgatcg agccgcgcca gcagtgcggc
     2221 tggcggtagc agcttgatgc gcgccgccgc cagctcgatg gccagtggca agccgtccag
     2281 ccgggcacag atct
//
LOCUS       DASUGJ010000286         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_460140, whole genome shotgun sequence.
ACCESSION   DASUGJ010000286 DASUGJ010000000
VERSION     DASUGJ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460140"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..685)
                     /locus_tag="VFV93_06350"
     CDS             complement(83..685)
                     /locus_tag="VFV93_06350"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5164999.1"
                     /translation="MAVFEIECLLQSPDATWSVERWEKLPNDGNRYEVIDGVLYMSTT
                     PTVFHQWIVGNVVELVGISFHNREVAYALAGPVGVFISDRDLVQPDFLAVRRDRSHII
                     ANGRINGAPDLIVEVISQSDTHHDTNTKRVAYARADVPEYWIVRPATRDIMVYWQPEP
                     DFGDFAQSRLVAADGILESPTLPVSQRIADFFEGSPDSTL"
     gene            867..1862
                     /locus_tag="VFV93_06355"
     CDS             867..1862
                     /locus_tag="VFV93_06355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5165000.1"
                     /translation="MKLGLTLPNRGVLFGVTTPEEMLRMSEIADQSGAFASIWVGDSL
                     LGKPRMESMMLLAGIAARTKRVRLGPACMASFPLRDPVLLAYQWASLDLLAEGRTVLV
                     ACTGIVPQAGGRIEGQLYGLEPRDRVKRLIEWIALVKRLWTEDDVSFDGEQYRCEGIT
                     IEPKPAAKPRPPIWIASNAGRGVQMTNPTTDRALVERTLRRAVRHADGWETSLSNPDD
                     LRWRLEYIREQLAREGRDPSTFETHLYHNINVNEDQSAALDESKRFLDIYYTENYAPE
                     FVASWTATGSPEQCVAHLKVYQEMGFDEVTVRITGWDQFGQLDRVINEVVPLMGA"
     gene            complement(1968..2732)
                     /locus_tag="VFV93_06360"
     CDS             complement(1968..2732)
                     /locus_tag="VFV93_06360"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5165001.1"
                     /translation="MKIARYQANGGAQIGIVEDGVVYAASGDMFGTLTKGERVGALDD
                     VRLLTPVEPGKIVAVGLNYALHVTENDPTREVPTEPVIFMKPTSALLPHGGVIQMPPA
                     DRIDYEAELCIVIGKEAFRVSQADALSYVLGYTCGNDVSHRDYQRKDGQWVRAKGFNT
                     FCPLGPVIETELDPGNVAVQSRLNGELRQNSHTKHLIFNIPFLLEFITNVMTLNPGDV
                     IMTGTPEGVGPMQPGDTIEVEVEGIGVLRNTTAARE"
     gene            2840..>3195
                     /locus_tag="VFV93_06365"
     CDS             2840..>3195
                     /locus_tag="VFV93_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5165002.1"
                     /translation="MADQRLLRVGLELPVAERRARDDVPGWRDIREMALRAEAVGFDS
                     VWVEDHLLLRPVGQEPQGLWECWSVVAALAAVTRRVEIGTYVACTAFRNPALLAKMAD
                     TVDEISGGRLILGLGAG"
BASE COUNT          569 a          985 c         1042 g          599 t
ORIGIN      
        1 tctccgcttt ggtctcgtat ggcgttcaag cagggaggtc cttcgctgcg gctcaggacg
       61 acagggggag ccgccatcgg cattacaggg ttgagtcagg cgagccttca aagaagtcag
      121 cgatccgctg gctaaccggc agtgtcggcg attccagaat gccgtctgct gcaacgagcc
      181 tcgactgcgc gaaatcaccg aagtctggct ccggttgcca gtagaccatg atgtcgcgag
      241 ttgccggccg gacgatccag tattccggaa catccgctcg tgcgtaggca acccgtttcg
      301 tattcgtatc gtgatgagtg tcagattgtg agatgacttc gacgatcagg tccggcgcgc
      361 cgttgattcg accgttcgcg atgatgtgcg aacggtccct ccgaaccgcc agaaagtcag
      421 gttggacgag atcgcgatcg gagatgaata ccccgaccgg ccctgccagc gcgtatgcca
      481 cttccctgtt gtgaaaagat atcccaacaa gctctacgac gttgccgaca atccactgat
      541 ggaagactgt cggtgtcgtc gacatgtaca acacaccatc gatcacctca tagcgattgc
      601 cgtcgttcgg tagcttctcc catcgctcga ctgaccaggt tgcatcaggc gactggagta
      661 gacactcgat ttcgaacacc gccatgtcgc atctcctgac acagcgcgtc agcccgacgt
      721 tagcacagat caatcgtagc aaggacgagt cttagcgcca tcagacttgc agcgacggct
      781 ccgcagcgcg aagatgcaca gcgctaacac aagctgtcca actcgattca aactgcaagc
      841 tggtagacac gaaagcgagt gtggtcatga agctcggact cacccttccg aaccggggcg
      901 tgctgttcgg cgtcacgacg ccggaagaga tgttgcgcat gtcggagatc gccgatcagt
      961 cgggcgcgtt cgcgtcgatc tgggttggcg actcgctgct gggcaagccg cggatggagt
     1021 cgatgatgtt gctggccggg atagcggccc gcacgaaacg tgtccggctc ggaccagcct
     1081 gtatggccag ctttccgttg cgcgatcccg tcctgctcgc ctaccagtgg gccagcctcg
     1141 acctgctggc cgagggtcgg acagttctgg ttgcctgtac cggtatcgtt ccgcaggcag
     1201 gcgggcgcat cgagggccag ctctacgggc tggagccgcg cgaccgggtc aagcggctga
     1261 tcgagtggat cgcgctcgtg aaacggctct ggaccgaaga cgatgtcagc ttcgacggtg
     1321 agcagtaccg ctgcgagggc atcacgatcg agccgaaacc ggccgcgaag ccgcgcccgc
     1381 cgatctggat cgccagcaac gccggacgtg gcgtccagat gaccaacccg acgaccgacc
     1441 gcgcactggt cgaacggacc ttgcgccggg ccgtccgcca cgccgacggc tgggaaacct
     1501 cgctctccaa cccggacgat ctgcgctggc ggctcgagta catccgcgaa cagctggcga
     1561 gagagggccg cgatccgtcg acgttcgaga cgcacctcta ccacaacatc aacgtcaacg
     1621 aggaccagtc agcggccctc gacgagtcga agcgcttcct ggatatctac tacacggaga
     1681 actacgcccc cgagttcgtc gcaagctgga ccgccaccgg ctcacccgaa caatgcgtcg
     1741 cccacctgaa ggtctaccag gagatgggct tcgacgaggt gaccgtccgc atcaccggct
     1801 gggaccagtt cggccagctc gaccgggtga tcaacgaggt ggtgccgttg atgggggcgt
     1861 gatggtttgg gactcgggaa ccgggattcg ggaacacggt cgtttgggaa tctaacaggc
     1921 gtcatccggc cccgaatccc ggttcccgaa tcccaaaccc cgcgtcccta ctcccgcgcc
     1981 gccgttgtgt tgcgcaggac gccgatgccc tcgacctcga cctcgatcgt gtcgccgggt
     2041 tgcatcgggc caacgccttc cggggtgccg gtcatgatga catcgccggg gttgagggtc
     2101 atcacgttgg tgatgaactc aagcaggaac gggatgttga agatcaggtg cttcgtgtgg
     2161 ctgttctggc gcagctcgcc gttcagccgc gactggacgg cgacgttgcc cgggtcgagc
     2221 tcggtttcga tgactgggcc gagcgggcag aacgtgttga agcccttggc gcgcacccat
     2281 tggccgtcct tgcgctggta gtcgcggtgg ctgacgtcgt tgccgcaggt gtagccgagc
     2341 acgtaggaga gggcatcggc ttgcgagacg cggaatgctt ccttgccaat cacgatgcag
     2401 agttcggcct cgtagtcgat ccggtcggcc ggcggcatct ggatcacgcc gccgtgcggc
     2461 agtagcgcgc tggtcggctt catgaagatg accggctcgg tcggcacctc tcgcgttggg
     2521 tcgttctcgg tgacatgcag cgcgtagttg agaccgacag cgacgatctt gcccggctcg
     2581 accggcgtca gtagcctgac gtcgtccagc gcgccgactc gctcgccctt cgtcaatgtt
     2641 ccgaacatgt cgccgctggc ggcgtagacg acgccgtcct cgacgattcc tatctgcgct
     2701 ccgccgttgg cctgatagcg tgcgatcttc actcgacatc ctcccgtatc ctgttcgagg
     2761 gaaccagcgc ccccgctgtc gctggcaact ggcggcgatt gtaaccggga atcgcgacag
     2821 gacatgggag gagtacgtgg tggcggatca gcgactgctc agggtcggtc tggaactgcc
     2881 ggtcgccgaa cgccgggcgc gtgacgacgt tcccggctgg cgcgacattc gggagatggc
     2941 gttgcgtgcc gaggcggttg gcttcgactc cgtctgggtc gaggatcacc tgctgctgcg
     3001 gccggtgggg caggaaccgc aagggctctg ggagtgctgg tcggtcgtgg ccgcgctggc
     3061 ggcggtgacg cgccgggtcg agatcggcac gtatgtcgcc tgtacggcgt tccgcaatcc
     3121 cgccctgctg gcgaagatgg ccgacacggt cgatgagatc agcggcgggc ggttgattct
     3181 cggtctcggc gccgg
//
LOCUS       DASUGJ010000287         5009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1572701, whole genome shotgun sequence.
ACCESSION   DASUGJ010000287 DASUGJ010000000
VERSION     DASUGJ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572701"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VFV93_06370"
     CDS             complement(<1..1188)
                     /locus_tag="VFV93_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HEX5165003.1"
                     /translation="MNQSPRQRSDRHATRNARRLPPVSGAAVAVAAGTTSSAIGVEPA
                     RRAPIQLRFNVEFILYSVIAGLAVLTRFWDLSSRAEHHDESLHAYYSWLFYVGEGYIH
                     DPLMHGPTLFHTNAFAYLLFGDNDYTSRLWPALLGVILVLMPAMLRGPRLLGRWGALT
                     CSALLLFSPSLLYQSRYIRHDPLVLVMTMAIVIAALRYLERPERKWIVTVAVMAGLLF
                     ATMEVSFIIAFVLVSFVAVISLWQISRRALALMIATGIGVLAVLKIMPALGAPAMPTI
                     PWEHPTSSNIRAFSQELVTHPIVLASLGVILLGVVLILGALDRVRDRSQPNWIESVFV
                     NAPDDTTSRAFYNLVRDRRSVWIGIALGLMLFAMFFTSMYTNMFGLGSGTVGALGYWL
                     GQHD"
     gene            1419..2342
                     /locus_tag="VFV93_06375"
     CDS             1419..2342
                     /locus_tag="VFV93_06375"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX5165004.1"
                     /translation="MDAGPLSADRRPGIPGRTSTLFLVSDGGGSTAEAVLDAATVQFP
                     DVSFSIIRRPGVRTREQVLAVVREAAAVQGIIVHTIVIQEIRHILVRECRQRIIPHFD
                     LIGPLIGHISQQVGLRPILRPGASRGIDVDYFKRIDAIQFTVQHDDGQSIATLDEADV
                     VLVGVSRSSKTPLSIYLSMRGWRVANIPIVLGVPPPRKLHEIDQRRIVAVTIDPPYLL
                     EIRRNRLQALGQTPDGEYTDPDKIEEELSYFRRTVRTGYPWPIVNMTGKSVEEAAKEV
                     IAVIAGQQADGLADGLKELDEVPPRYYEEQP"
     gene            complement(2454..2684)
                     /locus_tag="VFV93_06380"
     CDS             complement(2454..2684)
                     /locus_tag="VFV93_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165005.1"
                     /translation="MDDLVQQLQVKAGLSPEQAQKAAGVVADFLSNNVDADQLRSIAG
                     NIPGLSNYSDKIPDNIGDTLGNAARGLFGKKD"
     gene            complement(2782..3465)
                     /locus_tag="VFV93_06385"
     CDS             complement(2782..3465)
                     /locus_tag="VFV93_06385"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5165006.1"
                     /translation="MRIDLGTDVFGTDGEKVGAVDKIVIDPGTKQIHQFVVHRGFLTR
                     ENKLVDIAMVSGQDDQGLRLDLTSDQVNELPDYIEERFVQVPEHDVDAIPFILPTAMG
                     AGGYLYGAPYAGRGYEGRQDSFFDAAPSVAPVIEDESNIPETDVMISEGTDVYGADGD
                     KVGSVGEVLVSENGAIQGFVVSKGVIFKKDVRVPIDWVESADEAQIRLNVTSAEAEAG
                     AFNIEDSTL"
     gene            3659..4828
                     /locus_tag="VFV93_06390"
     CDS             3659..4828
                     /locus_tag="VFV93_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5165007.1"
                     /translation="MSAQESGSDDSRPSGRGLPVSSDIHQMAIRQALPIYYGLLFALL
                     TAGAILLLWWLKDVLLLLFISVLFAAAVSRPSAYLERIGIPRGFAAIAVYLASFAILV
                     AVGWFVVPTAVSQVATLADNAPAYADRYDNLRASYDKLAEKYPTLGTFDEQVGSIQER
                     IINSVAARLLDLPMRLFSIFLDTLAVFVISMLVVTSRERLLAFILAMVHPDHRETTQD
                     VLVKMWQRLGHYLRAKLIVMAIVGAITYVVLLLIGVPYPLMLAIIVAFGELIPRVGPW
                     IARIPLLAIALLDGWVTFGLTFAASIIIENLKGMVISPFVEGDQLDIPPLLVFVAVLV
                     GAALLGPAGAFVAVPAAAMVQVVFEEVIIPWRRRQLAPAEEALATNAGPVKTDDM"
BASE COUNT          870 a         1550 c         1646 g          943 t
ORIGIN      
        1 atcgtgctgg ccgagccagt agccgagcgc gccaacggtg ccgctgccca ggccgaacat
       61 gttggtgtac atgctggtga agaacatcgc gaacagcatc agccccagcg cgatcccgat
      121 ccagaccgag cgccggtcgc gaacgaggtt gtagaacgcg cgcgaggtcg tgtcgtcggg
      181 tgcgttgacg aagacgctct cgatccagtt cggctggctc cggtcgcgaa cgcggtcgag
      241 tgcgccgagg atcaggacga caccgagcag gatgacaccc agcgaggcca gcacgatcgg
      301 atgcgtgacg agttcctgcg agaaggcgcg gatgttggag ctggtcgggt gctcccacgg
      361 gatcgtcggc atggccggtg cgccgagcgc cggcatgatc ttcaggacgg ccaggacacc
      421 gattccggtc gcgatcatca acgccagtgc ccgacggctg atctgccaga gtgagatgac
      481 ggccacgaac gagaccagaa cgaaggcgat gatgaaggaa acctccatcg tcgcgaacag
      541 cagcccggcc atgacggcga cggtcacgat ccacttgcgc tcgggtcgct ccagatagcg
      601 cagtgccgcg atcacgatcg ccatcgtcat caccagcacg agcgggtcgt gccggatgta
      661 gcgcgactgg tagagcaatg atggcgagaa gagcagcagc gccgagcagg tcagcgcgcc
      721 ccaacgcccc agcaggcgtg gtccgcgcag catggccggc atcagaacca ggatgacacc
      781 gagcagcgcc ggccagaggc gtgacgtgta gtcgttgtcg ccgaagagca ggtaggcgaa
      841 cgcgttggtg tggaacagcg tcggcccgtg catgagcggg tcgtgaatat agccctcgcc
      901 gacatagaag agccacgagt agtaggcgtg caggctctcg tcgtggtgct cggcgcgcga
      961 ggacaggtcc cagaagcggg tcaggacggc gaggccggca atcacgctgt agaggatgaa
     1021 ctcgacgttg aagcgcagct ggattggcgc gcgccgtgcc ggctcaacgc cgattgcgga
     1081 ggacgtcgtc ccggccgcga cagcgacggc agcgccgctg actggcggca gccggcgagc
     1141 gtttcgagtg gcgtgccgat cggatcgctg gcgcggagac tggttcatcg gttgaagatc
     1201 accccgtggc gaacggccgg caggacaacg tcggcatcaa gaatcgtgcg gcgccgcgga
     1261 cgcagtgaac catggatcac cgaagcggcc aaccggccgt gaaagtgtac acgcgtccga
     1321 aatccccgtc atactgcgcg gcaaggctcg acaatgctcg ttacaatgcc cgccaggcgg
     1381 aatggctacg ctggacaacg tttcacggag gttgccgatt ggatgcagga ccgctttcgg
     1441 ccgatcgccg gcccggcata cccgggcgga cctcgaccct ctttctggta tcggacggtg
     1501 gcggctcgac cgccgaggcc gtcctggacg ccgcgacggt ccagtttccg gacgtctcct
     1561 tctcgatcat ccgacggccc ggcgttcgta cccgcgagca ggtgctggcg gtggtacgcg
     1621 aggccgcggc cgttcagggc atcatcgtcc acacgatcgt cattcaggag atacgccaca
     1681 tcctcgtccg cgagtgccgc cagcgcatca ttccgcactt cgatctgatc gggccgctga
     1741 tcggccacat ctcccagcag gtgggcctgc ggccgatcct gcggcccggt gcgtcgcgcg
     1801 ggatcgatgt cgactatttc aagcggatcg acgcaatcca gttcaccgtt cagcatgacg
     1861 acgggcagag catcgcgacg ctggatgagg cggatgtcgt gctggtcggc gtctcgcgct
     1921 cgtcgaagac accgctctcg atctacctct cgatgcgggg ctggcgggtt gccaacattc
     1981 cgatcgtgct gggtgtcccg ccgccgcgca agctgcacga gatcgaccag cgccggatcg
     2041 ttgccgtgac gatcgatccg ccctacctgc tggagattcg ccgcaaccgg ctccaggcac
     2101 ttgggcagac gccggatggg gaatacaccg atccggacaa gattgaggaa gagctatcct
     2161 atttccggcg aacggtgcgg actggctatc cgtggccgat cgtcaacatg accggcaagt
     2221 cagtcgagga ggccgccaaa gaggtcatcg ctgtcattgc cggccagcag gccgacggac
     2281 tggcggacgg gctgaaggaa ctcgacgaag tcccaccccg ttactatgaa gaacagccct
     2341 gatacgcatt ccggtagggg cgaggcattg actcgcccgg ctccccgccc gacgtgttta
     2401 ggcgggacac aagggtgagt cggtgcctcg cccctacaga ggaaaggggt gcgtcagtcc
     2461 ttcttgccga agagacctcg cgccgcgttg ccgagcgtgt cgccgatgtt gtcggggatc
     2521 ttgtcgctgt agttgctgag gccggggatg ttgccggcga tcgagcgcaa ctgatccgcg
     2581 tcgacgttgt tcgagaggaa atcagcgacg acgccggctg ccttctgggc ctgctccggt
     2641 gacagtcctg ccttgacctg tagctgctga accagatcgt ccatgatgcg tccctccctg
     2701 acagtgcgat gcgaatcgac attcagcccc gcagttggcg gggctgctgc cagtctaccg
     2761 gatcggcgct ggatttcgtc gtcagagggt gctgtcttcg atgttgaacg cgcccgcttc
     2821 ggcctcggcc gaggtgacgt tgagccggat ctgcgcttca tcggctgact cgacccagtc
     2881 gatcgggacg cgcacgtcct tcttgaagat gacgcctttg ctgacgacga atccctggat
     2941 cgcgccgttc tcgctgacca gtacttcgcc aacgctgccg accttgtctc cgtctgcgcc
     3001 gtagacgtct gttccctcag aaatcatcac atcagtctcc ggtatgttcg actcatcctc
     3061 gatgaccggc gcgacactcg gcgccgcgtc gaagaagctg tcctgccggc cttcgtagcc
     3121 acggccggcg tagggcgcgc catagaggta gccgccggcg cccatcgccg tcgggaggat
     3181 gaaggggatc gcgtccacgt catgctcagg gacctggacg aagcgctctt cgatgtagtc
     3241 cggaagctcg ttcacctggt ccgacgtcag gtcaagccgc agcccttgat cgtcctgacc
     3301 gctgaccatt gcgatgtcga cgagtttgtt ctcgcgcgtc aggaaccctc ggtgaacgac
     3361 gaactggtgg atctgctttg tgccgggatc gatgacgatc ttgtcgacgg cgccgacttt
     3421 ttcgccgtcg gtgccgaaca cgtccgtccc cagatcgatc ctcatttcag cctccatatc
     3481 acgtggttcg tatgcagatt tcaggtctga ctggcgcaat ctatatgcca tgcatttcat
     3541 gatggatcga tcgggtgaga gccatcgttg ctgggtgggg tgggggaacg cgcggcatgc
     3601 ctcttgatca gaggcggcat cgatccccat gttggggaag ggcaagaaag tgggcgtcat
     3661 gtcggcacag gaatcaggtt cggacgattc gcgaccgtcc ggtaggggtc tcccggtttc
     3721 ctccgatatc caccagatgg cgattcgcca ggcattgccg atctactatg ggctgctatt
     3781 cgcgctgctg acggcgggag cgatactact gctctggtgg ctgaaggacg tgctgctgct
     3841 gctcttcatc agcgtcctgt tcgccgctgc cgtctcgcgg cccagcgcct atctggagcg
     3901 gatcggcatc ccgcgcgggt tcgcggcgat cgcggtctat ctggcgtcgt tcgcgatcct
     3961 ggttgccgtc ggctggtttg tcgtgccgac agccgtctcg caagtggcga cgctggccga
     4021 caacgctccg gcatacgcgg atcgctacga caatctgcga gcgtcgtacg acaagctggc
     4081 cgagaagtac ccgacgctcg ggacgtttga cgagcaggtc ggctcgattc aggagcggat
     4141 catcaacagc gtagcagctc gacttctcga cttgccaatg cggctgttct cgatcttcct
     4201 cgacacgctg gcggtcttcg tgatctcgat gctggtcgtg acgtcgcggg agcggctgct
     4261 ggcgttcatc ctcgcgatgg tgcaccctga ccaccgcgag acaacgcagg acgtgctcgt
     4321 caagatgtgg cagcggctgg gtcattacct gcgggcgaag ctgatcgtga tggcgatcgt
     4381 tggcgcgatc acctacgtcg tgctgctgct gatcggtgtg ccgtacccgc tgatgctggc
     4441 gatcatcgtc gcctttggcg agctgatccc gcgtgttggg ccgtggatcg cccgcatccc
     4501 gttgcttgcg atcgcgctgc tggatggctg ggtgacgttc ggcctgacgt tcgccgcctc
     4561 gattatcatc gagaacctga agggcatggt gatctcgccg tttgtcgagg gagatcagct
     4621 cgacatcccg ccgctgctcg tcttcgttgc tgtcctggtc ggcgcggccc tgctgggccc
     4681 ggccggcgcg ttcgttgccg tcccggccgc cgcgatggtc caggtcgtct tcgaagaggt
     4741 catcattccc tggcggcggc gacagctcgc gccggccgag gaggcgctgg caaccaacgc
     4801 tggtcccgtg aagacggacg atatgtaaac ctgtcgtcct gagtagtcga cccgcgtgaa
     4861 tttgccgtcc tgacaccggt cggctagcat cgcggtcagg atgaaaggag atcaccgtgt
     4921 ccatcaccca tcgcgtgcgc ttgaccgagg accaacggac cgacctgatc gcgctgaccc
     4981 gtcgcggcga gtcgcacgct cgacgcatc
//
LOCUS       DASUGJ010000288         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_877892, whole genome shotgun sequence.
ACCESSION   DASUGJ010000288 DASUGJ010000000
VERSION     DASUGJ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877892"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFV93_06395"
     CDS             <1..675
                     /locus_tag="VFV93_06395"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5165008.1"
                     /translation="YLLSVHMFQHLLFTLVAPPLLLLGTPGWMLRPLIQNRHVRSVAY
                     AITRPFPALAIFNGVVLITHLTPVVDLALQIHLFHFFVHVLLVATAILMWFPVLSPLP
                     ELPRIGPFAQLIYLFVQQLVPGVVASFLVFSDHVVYGFYATVPVRYWGLSVLDDQRWA
                     AVVMKLLGGTLLWGVMAVIFFRWFGEEDRATIARTRASTPAPNSPDVRWPEVEAELER
                     MGLTKR"
     gene            722..1342
                     /locus_tag="VFV93_06400"
     CDS             722..1342
                     /locus_tag="VFV93_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX5165009.1"
                     /translation="MRDRLGLPVLLPLLVVGSILLTIFSFGYVLLEIEETRQAWPIAI
                     TVAAGILLIAAFINSQPSVRGLPLYLISALPVAVILGVGLFYLIRPAPEGAEVPVAAV
                     IAPPGPLEEVATDNRFSQGEFTIVAGQQYTMNFSNQGSAIHNWMVLGVQGTNGQPIQT
                     QLLPGGQSESVNFTIADPGTYQFHCSVHPTEMVGELTVVSEAEAGA"
     gene            1400..1666
                     /locus_tag="VFV93_06405"
     CDS             1400..1666
                     /locus_tag="VFV93_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX5165010.1"
                     /translation="MATDNKFAPTQFNAKAGEQTSLTLENDGSAIHNLHVQNVTGANG
                     QPIATKLLPAGQSETIQFTIAQPGTYNIICDVHPAEMTAKLAVQ"
     gene            1682..2305
                     /locus_tag="VFV93_06410"
     CDS             1682..2305
                     /locus_tag="VFV93_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165011.1"
                     /translation="MAPNDRGVLVVSPTLSRMALVLGIAVAAVGAWGRRIWTIESDFE
                     TLGVIVLMSGGLFVALLGLYGTVREPRSADPAIAKRQDILARIGWRATFPPVVAGATM
                     IGFFALTDRWHELANPVQSQVALFAGVLGGLVGMLADRITRWDLIVVPVGLIVALLLW
                     GDQLPFDSPTTTTGEIVALLIIAILIIGITVNIPQVVRGRRPVQAPS"
     gene            2411..>2622
                     /locus_tag="VFV93_06415"
     CDS             2411..>2622
                     /locus_tag="VFV93_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165012.1"
                     /translation="MSTQTAAPESTVTALQAAKQVFEENKTLTLISRNGDRAWGGKVY
                     FAEHEGDLYIALEQGRNFENVRANPKV"
BASE COUNT          459 a          840 c          802 g          521 t
ORIGIN      
        1 tatctgctca gcgtccacat gtttcagcac ctgctcttca cgctggtggc gccgccgttg
       61 ctgctgctcg gcacaccggg atggatgctg cggccgctga tccagaaccg tcacgttcga
      121 agcgtcgcct acgcgatcac ccgtccgttc ccggcgctcg ccatctttaa cggggtggtc
      181 ttgatcacgc acctcacgcc ggtggtggac ctggcgctgc agatccacct gtttcatttc
      241 tttgtgcatg tgcttctcgt cgcgacagcc attctgatgt ggttccctgt gctcagcccg
      301 ctgccggaac tgccgcgcat cggacccttc gcacagctga tctacctgtt cgtgcagcaa
      361 cttgtgcccg gtgtggtcgc atcattcctc gttttctccg accacgttgt gtatgggttc
      421 tacgccacgg tgccggttcg atactggggt ctctccgtgc tcgacgatca gcgctgggcg
      481 gcggttgtaa tgaagctgct gggtggcacg cttctttggg gcgtgatggc agtcatcttc
      541 tttcgctggt ttggcgaaga ggatcgggcg accatcgctc gaactcgagc ctcgacgccg
      601 gcgccgaatt ctcccgacgt gcgatggccg gaggtcgagg cggaattgga acgaatgggg
      661 ctaacgaaac ggtaggcggc gcggaggaac cgcgcgcacc gttgttgctg gagacgacgc
      721 aatgagagat cgtttggggc tgccggtgct gcttcccctg ctggtcgtag ggtcaatcct
      781 gctgaccatc tttagctttg gctacgtcct cctcgagatc gaggagacac gacaagcctg
      841 gccgatagca atcaccgtcg cagcagggat cttgctcatc gcggcattta tcaacagcca
      901 gccatccgtc cgtggattgc cgctctactt gatctccgcg ctgccggttg ccgtcatcct
      961 cggcgtcggc ctcttctatc tgatccgccc tgccccagag ggagcggaag ttcccgtcgc
     1021 ggccgtaata gcgccgcccg gcccactcga ggaggtcgcg acggacaatc ggttctcgca
     1081 gggtgagttc accatcgtcg ccggccagca atacacgatg aacttcagca accagggcag
     1141 cgcaatccac aattggatgg tccttggcgt gcagggtacc aacggccagc cgattcagac
     1201 acagctgctc cccggcgggc agtccgagtc ggtcaacttc accatcgccg atccgggcac
     1261 ctatcagttc cactgtagcg tccacccaac ggaaatggtt ggcgagctga ccgtggtctc
     1321 cgaggcagaa gccggcgcgt aggccggagc ggccactgga tcgggcggcg gcaaccctgg
     1381 tcctggcacg atcgtcgctg tggcaacgga caataagttc gcaccgaccc agttcaacgc
     1441 aaaggccggc gagcagacct cgctgaccct ggagaacgat ggttcggcga ttcacaacct
     1501 gcacgttcag aatgtgaccg gtgcgaacgg tcagccgatc gcgacgaagc tcctgcctgc
     1561 tggtcagtct gagacgattc agttcacgat cgcccaaccg gggacatata acatcatctg
     1621 cgatgtccat ccggccgaaa tgaccgccaa actcgcggtg caatagatgg aacccggagc
     1681 cgtggctccg aatgatcggg gtgtcctcgt ggtgtctccg accctcagcc gtatggcact
     1741 tgtcctcgga atcgcagtcg ccgcggtcgg cgcctgggga cgacggatct ggacgatcga
     1801 gagcgatttt gaaacgctcg gcgtgatcgt gctcatgtcc ggcggactgt tcgttgcgct
     1861 cctcggtctg tacggcaccg tccgagagcc gaggtccgcc gatcctgcca tcgccaagcg
     1921 ccaggacatc ctcgcgcgga tcggctggcg cgcgaccttt cccccggtgg ttgccggcgc
     1981 gacgatgatc ggcttcttcg ccctcaccga ccgatggcac gagctcgcca atccggtcca
     2041 aagccaggtc gccctgttcg ccggcgttct cggcgggctg gtaggcatgc tggccgaccg
     2101 catcacccgt tgggacctga tcgtcgtgcc ggtgggtttg atcgtcgcac tgctgctatg
     2161 gggcgaccag ttgccgttcg acagcccgac gacaacgacc ggtgagattg tcgcgctcct
     2221 catcatcgcc atcctcatta tcggcattac cgtgaatatt ccccaggtgg ttcgcggccg
     2281 gcggccggtc caagcgccgt cgtaacccgc ccgagtcccc accgacgccc ccatcaggcg
     2341 ggactcgggc gcatcgactc tgtgtcctgt tcggcaacta tcatggaacc aaccgcacga
     2401 gaggcgcgag atgtcgacac aaacggcggc accggagagc acggtcacag cgctgcaggc
     2461 ggcgaagcag gtcttcgagg aaaacaagac cctgacgttg atcagccgca atggcgatcg
     2521 ggcctggggc ggcaaggtct attttgccga gcacgagggc gatctctaca tcgcgctcga
     2581 acagggccgc aacttcgaga acgtccgagc gaacccgaag gt
//
LOCUS       DASUGJ010000289         4202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1572991, whole genome shotgun sequence.
ACCESSION   DASUGJ010000289 DASUGJ010000000
VERSION     DASUGJ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572991"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VFV93_06420"
     CDS             <1..1091
                     /locus_tag="VFV93_06420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5165013.1"
                     /translation="EGIDAHYAKGGQLEIARATYDLPMLEDLYDEYRAIGLEDHYQLL
                     DANQTEERIRVAGAVGSFWNKEGAAIQPARLTRGLAHAVERHGGVIYEQTEVLDYVPG
                     PLPRLDTARGNISARAIVLAGEGYLSRLPKLRRRIVPATSHIVITEPLSDDLWQQIGW
                     ERREVVGGFGTTGAYLNHTADGRIAFGPYRGKVPFNSRITDALDRPEDVFEHGRKSAL
                     EWFPMLRDAGVRFTHSWGGVFGVPRDHMPIMRFDRQTGVAMGYGYSGEGVATANLSGR
                     VLADLITERETELTDLPTTRHLPVNWEPEPFRWAGYKIVRRARYRADEEAERTGRHRE
                     KPSLGQRLWSFDPPRAWRSVMRMDGGND"
     gene            1123..2361
                     /locus_tag="VFV93_06425"
     CDS             1123..2361
                     /locus_tag="VFV93_06425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5165014.1"
                     /translation="MTETPPTAAALGLMDGYPAPPDKRVTRENLMQPPYNRWGFQHMR
                     ELMPTAEVYRGPGPVRELPRRPLDLSDLQFVAADGSQRTVKEMLDLSYADAFVVLHNG
                     ALVYEQYPNGMGPASQHLMMSVTKSLVGTLALQLAHEGTIDPSRMVADYLPELTGSAW
                     EDASVQNALDMTTGIRFDEVYDFADGALGDLARFAIATGFSAMPEGYDGPRTVPELLP
                     QFPKAGNHGEAFHYVTPNTDVIGWIIARVTGQPFSRIFGERIWSRLGAERDAYMIRDA
                     IGMQMAGAGFNATARDLARFGQLLLDDGVCDGQQLIAPEVIAVVRDGGDREAFARGTE
                     EYGPLWQGWSYRAFWWVTHNSHGAFTGIGINGQWLYIDPTARVVIVQQSSYPTSDQPE
                     ADDVCLPGFHAIAQHLMDRQ"
     gene            2384..3334
                     /locus_tag="VFV93_06430"
     CDS             2384..3334
                     /locus_tag="VFV93_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5165015.1"
                     /translation="MTKPQMHPDMQVLLQAREQTNAKLGPRGDAPEDARRWWNTYAAI
                     LAQPHPEDMHVYDRSIPTEEHDVPVRIYRPNGVSDAAPCIVYMHGGGFMMGDLDSSDS
                     NAWGYAEEVGAVVVSVDYRLTPEHPYPAAFNDCYGVLCWLAANPGELGIDAGRIAVAG
                     DSAGGNLGAALALAARDRGGPQIAAQALIYGSFGLDQDSGSYLEFAEGLGLTLASTQK
                     FRRLYLPGNLDSDDPYARPIRARDFSGLPPALVHSAEMDPIRDDGRAYAARLALAGNL
                     VTYREARGMVHGFLRARFTGATARVEFEAACAFLREQLAG"
     gene            3366..>4202
                     /locus_tag="VFV93_06435"
     CDS             3366..>4202
                     /locus_tag="VFV93_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5165016.1"
                     /translation="MEVLVQRLNALCDEQPFQTGWFLKDARTGAEADRAGDVVGPSAS
                     TRKIAVLMTALAEVNAGRLDLDQLVTIQERYQHNNSGVFQHLTAGTLLPLRDALAMMI
                     IVSDNTCTGMIVDMLGLDTINAFSRSAGMVGTTHRDNIPSGSLSWDHPVEATNATTPN
                     DVGRLLDQILRGSQDEAVARRLGCTPELCRYAIEIMTWQQITNRLPLLMPPPPTATMA
                     HKTGRGVRNFSDAGIIFRRDEPLYVLTVYTDQVPVVLPDGGAGRGAAYLLTAQLCREC
                     WDY"
BASE COUNT          731 a         1403 c         1423 g          645 t
ORIGIN      
        1 tggagggaat cgacgcgcac tacgcgaagg gcggtcaact ggagatcgcc cgcgcgacct
       61 atgacctgcc gatgctggag gacttgtacg acgagtaccg cgcgatcggg ctggaggacc
      121 actaccagct gctcgacgcc aaccagaccg aggaacgcat ccgcgtggcc ggcgcggtcg
      181 gctcgttctg gaacaaggag ggcgcggcga ttcaaccggc ccgactgacg cgtggcctgg
      241 cgcacgccgt cgagcgccac ggcggcgtga tctacgagca gaccgaagtg ctcgactacg
      301 taccggggcc gctgccgcgt ctcgacaccg cgcgcggcaa catctccgcc agggcgatcg
      361 tgctggccgg tgagggctat ctgtcacggt tgccgaagct ccgccggcgg atcgtcccgg
      421 ccacctcgca tatcgtcatc accgagccgc tgtcggacga cctctggcaa cagatcggct
      481 gggaacgccg cgaggttgtc ggtgggttcg gcacgaccgg cgcctatctc aaccacacgg
      541 ccgacgggcg gatcgcgttt ggtccctatc ggggcaaggt gccgttcaac tcgcgcatta
      601 ccgacgcgct ggaccggccg gaggacgtct tcgagcacgg gcgcaagtcg gcgctggagt
      661 ggtttccgat gctgcgcgac gccggggtgc gcttcaccca ttcctggggc ggtgtcttcg
      721 gcgtcccacg cgaccacatg ccgatcatgc gtttcgaccg gcagaccggc gtggcgatgg
      781 gctacggcta ctcgggcgag ggtgtcgcaa cagcgaacct ctccggccgg gtgctggcgg
      841 atctgatcac tgagcgcgag acggagttga ccgatctgcc gacgacacgc catctaccgg
      901 tcaattggga gccggagcca ttccgctggg ccggctacaa gatcgtgcgc cgtgcccgct
      961 accgggctga cgaggaagcg gagcggaccg gccggcaccg cgagaaaccg agcctcggac
     1021 agcgcctgtg gagcttcgat ccgccccgcg cctggcggag cgtgatgcgg atggatggag
     1081 gaaacgactg atgcgtactg aaacgacaag aggacgagca cgatgacgga gacgccaccg
     1141 acggcggcag cgctcgggct gatggatggc tacccggcgc cgccggacaa gcgggtcacg
     1201 cgtgagaacc tgatgcagcc gccctacaac cgctggggct ttcagcatat gcgcgagctg
     1261 atgccgacgg ccgaggtcta tcgagggccg gggccggtac gcgaactgcc gcgccgccct
     1321 ctcgacctca gtgacctgca gttcgtcgcg gcagacggca gccagcgaac ggtcaaggag
     1381 atgctcgacc tgagctacgc cgatgcgttc gtcgtcctgc acaacggggc gctggtctac
     1441 gagcaatacc cgaatgggat ggggccggcc agccagcatc tcatgatgtc ggttaccaag
     1501 tcgctggttg ggacgctggc gctccaactg gcccacgagg gaacgatcga cccgtcccgg
     1561 atggtcgcgg actatctccc ggagctgacc ggcagcgctt gggaggacgc gagcgtccaa
     1621 aatgcgctcg acatgacgac cggcatccgc ttcgacgagg tctacgactt cgcagacggc
     1681 gcgctcggcg atctggcccg tttcgcgatc gcgaccggct tcagcgccat gccggagggc
     1741 tacgacgggc cgcgcaccgt cccggagctg ctgccgcagt ttcccaaggc cggcaaccac
     1801 ggcgaggcgt ttcactacgt cacccccaac accgatgtca ttggctggat catcgcccgc
     1861 gtgaccggcc agccgttctc gcggatcttc ggtgagcgga tctggtcgcg gctcggcgca
     1921 gaacgggacg cgtacatgat ccgcgacgcg atcggcatgc agatggccgg cgccggcttc
     1981 aacgcgaccg cgcgcgatct ggcccgcttc ggtcaactgc tgctggatga cggcgtctgc
     2041 gacggtcagc agctcatcgc gccggaggtc atcgcggtcg tccgcgacgg cggcgaccgc
     2101 gaggcgttcg cgcgcggcac cgaggagtac gggccgctct ggcaggggtg gtcgtaccgc
     2161 gctttctggt gggtcaccca caatagccac ggcgcgttca ccgggatcgg gatcaacggt
     2221 cagtggctgt acatcgaccc gaccgcccga gtcgtcatcg tccagcagtc gagctatcca
     2281 acctccgacc agcccgaggc cgatgacgtc tgcctgccgg gcttccacgc gatcgcgcaa
     2341 catctgatgg accggcagtg accaccgcag ggaggagagc caggtgacga agccgcagat
     2401 gcacccagac atgcaggtgc tgctccaggc acgggagcag acgaacgcca agcttggccc
     2461 gcgcggtgat gcgccggagg acgctcgccg ctggtggaac acctacgctg ccattctggc
     2521 gcaaccgcat ccggaagaca tgcacgtcta cgaccggtcg atcccgaccg aggagcatga
     2581 tgttccggtc cgtatctacc gtccgaacgg ggtgagcgat gcggcgccat gcatcgtcta
     2641 catgcacggc ggcgggttca tgatgggcga tctcgacagc tcagacagta atgcctgggg
     2701 ctacgcagag gaggtcggcg cggtcgtcgt ctcggttgac tatcgcctga cgccggaaca
     2761 tccctatccg gccgccttca acgactgcta cggcgtcctc tgctggctgg ccgccaatcc
     2821 gggcgaactt gggatcgacg cgggacggat cgcagtcgcc ggcgacagtg ccggcgggaa
     2881 cctgggcgcg gcgctcgcac tcgcggcacg cgatcgtgga gggccgcaga ttgccgccca
     2941 ggcgctgatc tacggctcgt tcggactcga ccaggactcg ggatcgtacc ttgaattcgc
     3001 cgaggggttg gggttgacgc tggccagtac gcagaagttc cgccggctct acctgcccgg
     3061 caacctggac agcgatgatc catacgcgcg gccgatcagg gcgcgggact tctccggtct
     3121 gcctccggcg ctggtccact cagccgagat ggacccgatc cgcgatgacg gacgcgccta
     3181 cgcggccagg ctcgcgctgg ccggcaacct ggttacctac cgcgaggcac gcgggatggt
     3241 tcacgggttc ctccgcgcgc gattcaccgg tgcaacggcc cgcgtcgagt tcgaagccgc
     3301 ctgcgcgttc ctgcgcgaac aactggcagg atgatgctgc gagagcagca cgagaggagc
     3361 agccgatgga ggtgttggta cagcgactga acgcactctg cgatgagcag ccgtttcaga
     3421 ctgggtggtt cctgaaggac gcgcggaccg gcgccgaggc ggaccgggcc ggtgacgtgg
     3481 tcgggccatc ggccagcacg cgcaagatcg cggtgttgat gaccgccctg gccgaggtca
     3541 acgccggccg gctcgacctc gaccagctgg taacgatcca ggaacgctac cagcacaaca
     3601 actcaggcgt cttccagcac ctgacggccg ggacgctgct gccgttgagg gacgcgctgg
     3661 cgatgatgat catcgtctcc gacaacacct gtaccggcat gatcgtcgac atgctcgggc
     3721 tggacacgat caacgccttc tcgcgctcgg ccgggatggt cggcacgacc caccgggaca
     3781 acatcccatc cggctcgctc agttgggacc atccggtcga ggcgaccaac gcaacgacgc
     3841 cgaacgacgt cgggcggctg ctcgaccaga tcctgcgcgg ctcacaggac gaggctgtcg
     3901 cacgcaggct cggctgcaca ccggagctgt gccgctacgc gatcgagatc atgacctggc
     3961 agcagatcac caatcgcctg ccgctgctga tgccaccacc gccgacggcg acgatggccc
     4021 acaagaccgg ccgcggcgtg cgtaacttca gtgacgccgg catcatcttc cggcgcgacg
     4081 aaccgctcta cgtcctgacc gtctacacgg accaggtccc ggtcgtcctg cccgacggcg
     4141 gcgccggccg cggcgctgcc tacctgctga ccgcccagct ctgtcgcgaa tgctgggact
     4201 at
//
LOCUS       DASUGJ010000290         4304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_43685, whole genome shotgun sequence.
ACCESSION   DASUGJ010000290 DASUGJ010000000
VERSION     DASUGJ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43685"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFV93_06440"
     CDS             <1..423
                     /locus_tag="VFV93_06440"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165017.1"
                     /translation="ILTAQRDATQRTSSPTIDVLHDIKACAREMIGLVERGQLDDIGY
                     LLDESWRRKKALAAGISTPDIDRWYQEALDAGASGGKITGAGGGGFLMLYVDNDHREP
                     VIQRMSELGLVWVDTAFDTEGASVLLHEASMDAIASVR"
     gene            complement(504..1436)
                     /locus_tag="VFV93_06445"
     CDS             complement(504..1436)
                     /locus_tag="VFV93_06445"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5165018.1"
                     /translation="MRVIVTGGAGFIGSHVAERLLVAGHDVAIIDDLSTGHRSNVPEA
                     ATFFECDIRDERIAAIFAEFQPEAVCHLAAQMSVRISVEEPRRDASINVEGAVNLLEA
                     AVSTSRPKVIYASTGGAMYGEPRWLPCDEQHPVVPLSQYGISKHTVEHYLELYSGLYG
                     LNYTALRLPNVYGPRQDPHGEAGVVSIFAGQMLAGSGITIFGDGAQERDFVYVSDVAR
                     GFEIALTRGSRMSINLGSGRGTSVLEIRDELAALIGRPIETRFQPARPGEVYRIFLTG
                     DLAADQLGWQPEVSLAEGLSQTLDWVRESTVVAV"
     gene            complement(1488..2981)
                     /locus_tag="VFV93_06450"
     CDS             complement(1488..2981)
                     /locus_tag="VFV93_06450"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5165019.1"
                     /translation="MDSVTVDLSKGRLPQLTGSFEQDFAAVLSRRRLQRGLNVATLLL
                     TDTLSILLGLAIAYVIRFQTRWGLFYEHDVSPVHRYVLLALAALPLFLFVLACYQLYS
                     ERRIFDGAKEYPRIISAVTMAVMIVILFSFMFESGLVIARGWIVLSWISLIVTVAVGR
                     FLARRLIYRLHSAGRLGQRVLVIGSGDDLLGLADRVRQVPAAGLQIADVVDCSYLAGS
                     SDDASPSMAALIAATRADALLISAASVPQPILSRIVREVSRLPTALHLVPGMYEILTT
                     GVQAGEIHGLPVVTMNKVRITGLDLILKRMLDYIVALGVLLLTSPMLLLAALLVRTTS
                     RGPVLYGRPVVGQQGRIFVAWKFRTMRVDGDEILRSRPDLREELERHGKLVDDPRVTP
                     VGSFLRRWSIDELPQLLNVLAGQMSLVGPRMITEPELNHFGRWRENFSTVKPGLTGLW
                     QISGRSDLGYEDRVRLDMHYIRNYSIWIDIEILLRTVPAVLDGRGAY"
     gene            complement(2969..4165)
                     /locus_tag="VFV93_06455"
     CDS             complement(2969..4165)
                     /locus_tag="VFV93_06455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5165020.1"
                     /translation="MRILFILPYGPSETRVRSRMLLAELARRHAITLVGLCWNESDRQ
                     ALDDWAARGLEVHAIPHGLRSWVGGAYRAVRQPLQHVVSTSPALAGEVRRLIAEAQAA
                     GQPFDVVHVEHLRGAAAADLLAGFGVWTVYDAVDCISELARLTWKLNPAPITRLVAWR
                     EQQPTRRLERAIALASDCTTVVAERDAEALRELTPVARIEVIPNGVTCLPVPVELTCQ
                     PTVIFTGKLSYHANQAAIRWFLAEAWPWVTQAVPDAQLVVAGADPPGWLLDRADGVAI
                     TVIANPPEMASLIGEARVSIAPMPYSVGIQNKILEAMAAGVPVVATSAARSGIGPTGA
                     DALIHADDPREFAAEVIRVLTEDRLARQLGRLGYDYVRANHSWEAAASHFEALYAPER
                     VMQWTA"
BASE COUNT          820 a         1475 c         1361 g          648 t
ORIGIN      
        1 atcctgacgg cccagcgcga cgcgacccag cggaccagct ccccaacaat cgacgtcctg
       61 cacgacatca aagcctgcgc ccgcgagatg atcgggctgg tcgaacgcgg ccagctggat
      121 gacatcggct acctgctgga cgaatcctgg cggcgcaaga aggcgctggc ggccggcatc
      181 tcgacgccgg acatcgaccg ctggtaccag gaagccctcg acgccggcgc gtcgggcggc
      241 aagatcaccg gcgccggcgg cggcgggttt ctgatgctct acgtcgacaa cgaccaccgc
      301 gagccggtca tccagcgcat gagcgagctg ggcctcgtct gggtcgacac ggccttcgac
      361 accgaaggcg cgtcggtcct gctgcacgag gcaagcatgg atgcgatcgc aagcgttcgc
      421 tgatcgcgag tcattccaat aatccaatgc tcaatccggc cgggtacccg gccggattgg
      481 atattgagga ttgtgagtgt caatcagacc gcaaccaccg tcgattcccg gacccagtcc
      541 agcgtctgcg acagtccctc cgcgaggctg acctccggct gccagccgag ctggtcggcc
      601 gccagatcgc ctgtcaggaa gatgcggtag acctcgcctg ggcgtgccgg ttggaagcgc
      661 gtctcgatcg gcctgccgat gagtgccgcc agctcgtcgc gaatctccag cacgctggtt
      721 ccccgccctg agccgaggtt gatcgacatc cgtgatcccc tggtcagcgc gatctcgaat
      781 cccctcgcca cgtcgctgac gtagacgaag tcgcgctcct gggcgccgtc accgaagatg
      841 gtgatccccg atccggcaag catctggccg gcgaagatcg agacgacacc ggcttcgcca
      901 tgcgggtcct gacgcggccc gtagacgttc ggcagccgca gcgcggtgta gttgaggccg
      961 tacaggccgc tgtagagctc caggtagtgc tcaaccgtgt gcttcgagat cccgtactgc
     1021 gagagcggca cgaccgggtg ctgctcgtcg cacggcagcc agcgcggctc gccgtacatc
     1081 gccccgccgg tcgaagcgta gatcaccttc ggccgcgacg tgctgactgc cgcctccagc
     1141 aggttgacgg ccccttcgac gttgatcgag gcgtcacgcc gcggctcctc gaccgagata
     1201 cgaacgctca tctgggccgc caggtgacag accgcttccg gctggaactc ggcgaagatg
     1261 gcggcgatcc gctcgtcgcg gatgtcgcac tcaaaaaagg ttgccgcctc cggcacgttc
     1321 gagcggtggc cggtcgagag gtcgtcgatg atcgcgacgt cgtgaccggc caccagcagc
     1381 cgctccgcga cgtgcgaccc aatgaacccc gcgccaccag taacaatgac ccgcatgata
     1441 cccacgtccc ttcagagaca atccgctgat cgcaccgtca gcgacagcta gtaggcgcca
     1501 cggccatcca gaacggccgg gacagttcgc agcagaattt cgatatcgat ccagatcgag
     1561 tagttgcgga tgtagtgcat gtccagccgg acacggtcct cgtagccgag gtcactccgg
     1621 ccactgatct gccagaggcc ggtcaggcca ggcttgacgg tcgagaagtt ctcgcgccag
     1681 cggccgaagt ggttcagctc cggctcggta atcatccgcg gcccgaccag gctcatctgc
     1741 ccggccagga cgttgagcag ttgcggcaac tcgtcgatgc tccagcggcg caggaagctg
     1801 ccaaccggcg tgacgcgcgg atcgtcaacc aacttgccgt gccgctccag ttcctcgcgc
     1861 agatccggcc gggagcgcag gatctcgtcg ccatcgaccc gcatcgtgcg gaacttccag
     1921 gcgacgaaga tccgcccctg ctggccgacg accggccgcc catagagcac cggcccccgt
     1981 gaggtcgtcc ggaccagcag cgccgccagc aacagcatcg ggctggtcag cagcagcaca
     2041 cccaaggcca cgatgtagtc gagcattcgc ttcaggatca gatcgagacc ggtaatccgc
     2101 accttgttca tcgtcacgac cggcagaccg tggatctcgc cggcctggac gccggtcgtc
     2161 aggatctcgt acatacccgg caccaggtgc aacgccgtcg gcagtcgcga cacctcacgc
     2221 acgatccgcg agaggatcgg ctgcggcacc gaggccgcgc tgatcagcag cgcgtcggcg
     2281 cgggtcgccg cgatcaaggc tgccatcgag gggctggcat cgtcggacga tccggccaga
     2341 tacgagcagt cgacgacatc ggcgatctgt agcccggcgg ccggcacctg gcgaacccga
     2401 tcggcaaggc caagcaggtc gtcgccgctg ccgatcacca gcacacgctg accgagacgt
     2461 ccagcgctgt gcagccggta gatcagccgg cgcgccagaa accgaccgac ggctaccgtc
     2521 acgatcagcg atatccacga cagcacgatc cagccacggg cgatgacgag ccccgattca
     2581 aacatgaagc tgaacaggat gacgatcatc accgccatcg tcacggccga gatgatgcgc
     2641 gggtactcct tggcgccgtc gaagatgcgc cgctcgctgt agagctggta acaggccagc
     2701 acaaacagga acagcggcag cgccgccagc gccagcagga cgtagcggtg gactggagag
     2761 acgtcgtgct cgtagaacaa tccccaacgg gtctggaagc ggatcacata ggcgatcgcg
     2821 agcccgagca ggatgctcaa ggtatcagtc agcagcaacg tcgcgacatt cagcccgcgt
     2881 tgcagccggc ggcgcgagag gacggccgcg aaatcctgct cgaacgagcc ggtcaactgc
     2941 ggcaaccgac ctttagagag atcgaccgtt acgctgtcca ttgcattacc cgctccggcg
     3001 cgtagagcgc ctcaaaatgc gacgcggccg cttcccagga gtgattcgcc cgtacgtagt
     3061 cgtaaccgag ccggccaagc tgccgggcca gccgatcttc agtcaggacc ctgatcacct
     3121 cggccgcgaa ctcgcgcggg tcgtccgcgt gtatcagggc gtctgccccg gtcgggccga
     3181 tgccgctccg ggcagccgac gtcgcgacga ccgggacacc agccgccatc gcctccagga
     3241 tcttgttctg gataccaacg ctgtacggca tcggcgcaat cgacacgcgc gcctcgccga
     3301 tcaggctggc catctctggc gggttggcga tgactgttat tgccacgcca tcggcccggt
     3361 ccagtaacca gccaggcgga tcggcgccgg cgaccacgag ctgtgcatct gggacggcct
     3421 gcgtcaccca cggccaggcc tcggccagga accagcgaat cgccgcctgg ttggcgtggt
     3481 aggagagctt gccggtgaag atgacggttg gctggcaggt cagctcgacc ggcaccggca
     3541 ggcaggtcac gccgttcggg atcacctcga tccgcgccac tggggtgagc tcccgcagcg
     3601 cctccgcatc ccgttcggca acaacggtcg tgcaatcgga cgccagcgca atcgcccgtt
     3661 caagccggcg ggtgggttgc tgctcgcgcc aggcaaccag ccgcgtgatc ggcgccgggt
     3721 tgagcttcca ggtcagccgc gccagttctg agatgcaatc aacggcgtca tagaccgtcc
     3781 agacgccaaa cccggccagc agatcggcgg cagccgcccc acgcagatgc tcgacatgaa
     3841 cgacatcgaa cggctggccg gctgcctgcg cctcggcgat cagccgccgc acctcaccag
     3901 ccaacgccgg tgaggtcgag acgacatgct gcaatggctg gcgaacagcg cgatacgcgc
     3961 cgccgaccca cgagcgcagc ccatgtggga tggcgtgaac ctccagtcca cgggcggccc
     4021 agtcgtccag cgcctggcgg tcgctctcgt tccagcagag acccaccagc gtgatcgcat
     4081 gtcgtcgtgc gagttctgcc agcagcatcc gcgagcgcac acgcgtctcg ctcggcccat
     4141 acggcaagat gaagaggatt cgcaaaccac gcctccaacg ggcgaagccg aagcgtcgat
     4201 catgcatcca gcctagtggt gaggggcgtt ctgatgggtt atccagccgt caatcgaggg
     4261 tgacaatcac gtcacatcgc gggcagacgc gatcgaaaag ccca
//
LOCUS       DASUGJ010000291         4364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_738700, whole genome shotgun sequence.
ACCESSION   DASUGJ010000291 DASUGJ010000000
VERSION     DASUGJ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738700"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFV93_06460"
     CDS             complement(<1..523)
                     /locus_tag="VFV93_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165021.1"
                     /translation="MRTRALAIVLIVVLACGSLVVWSRPSLAQPSATFTVTNTIDAVD
                     SAPGDGLCDIGDDSCTLRAAIQEANALAGADTIIVPAGLYALTISGTFEDLAATGDLD
                     IAGDLLIQGAGAVSTIVDGGQLDQVFQIIGTSVVTFSGLTIQNGNAAGWVDSSGNRHG
                     PMGGAILNPAGSIT"
     gene            complement(730..987)
                     /locus_tag="VFV93_06465"
     CDS             complement(730..987)
                     /locus_tag="VFV93_06465"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX5165022.1"
                     /translation="MQVVTKVTVVGPYVLDVTFRNGEIRQVDLEDSLWGPVFEPLRDP
                     AVFALAYVDQELGTVVWPNGADIAPEYLRSHGTSLTPEAAF"
     gene            complement(1079..1312)
                     /locus_tag="VFV93_06470"
     CDS             complement(1079..1312)
                     /locus_tag="VFV93_06470"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEX5165023.1"
                     /translation="MTLDPFIESVKATTLPELSRFRGIVVAMYYLDHDPPHVHAAHGG
                     KRTRVTISPPAIMDEQDRIDPRGAVFARVDSTP"
     gene            complement(1409..1558)
                     /locus_tag="VFV93_06475"
     CDS             complement(1409..1558)
                     /locus_tag="VFV93_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165024.1"
                     /translation="MMFKERLLFVVLAAAIAASLFIGLSVIIDNMNAAPLHPAVIVGR
                     SSIFA"
     gene            complement(1723..3327)
                     /gene="ggt"
                     /locus_tag="VFV93_06480"
     CDS             complement(1723..3327)
                     /gene="ggt"
                     /locus_tag="VFV93_06480"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5165025.1"
                     /translation="MGGMVVAPQVPAVETGVDVLRNGGNAVDAAVTAAFVQMVVDPQM
                     CGIGGFGAATVRTAAGEEVTIDFNGTAGSKATPEMWQDIVIEQDWTGYGYHLEGKVND
                     VGYGSIMTPGTVAGMAELLDRFGTISWKDALQAAIRISDAGYVVTPELWRIWNTPGYG
                     LHISFGDRLQATAPSRAIYFKPDGSGYKPGERIMNPDHTRTLQRLADGGPREFYEGDL
                     AKKLAADLAANGSFITADDLASYKVRVSEPIRIDYRGQTILTNQPAGGGICMAQTLKI
                     IEHEDIAALGLNSVEYIDLVGHAMKAAYHDWYAHVADPAFVDVPVGMLLSDEHADKWY
                     ARIKAGEQFSVPRYPEAPTTTNITVVDDAGNAIALTHSLGSSSGVVPAGYGFTWNNIM
                     NAANPQPGLPNSIAPGKSRITGMCPTIVLRDGQVVLALGAPGGTRIITGVLQTLLNVL
                     DHGLSPVEAVSAPRFDCQSDTLDCEARIPSWTKAALAERGFTIWPNSAPYGSFALVQA
                     ITRDPATGTLRGGADPRGGGAAMSTN"
     gene            3511..4230
                     /locus_tag="VFV93_06485"
     CDS             3511..4230
                     /locus_tag="VFV93_06485"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEX5165026.1"
                     /translation="MHRIASLLLIALLLVACGGGDDDANDSSSNSAPTATIASSTTVA
                     GSTAAPAQPTTQGAAATMPTESVSTPTVSTGGGGEDVEGAPTNASADFPDVRVPLEAG
                     SSGEVGSMDPDPFAATDTLDENSKVKVTINEVLDPATVDGTIFSPEEGNRWFAINVTM
                     EATGSEVANTGEWTLSTTDGAEYTNSIVLGSTPDITYGSIEASTSTEGIVVFEIPEGA
                     EVAWILMSPTIFVGGNLVFVN"
BASE COUNT          804 a         1356 c         1423 g          781 t
ORIGIN      
        1 ctgtgataga cccggctgga ttgagaatgg cgccgcccat cggcccatgt cgattcccgg
       61 atgaatcgac ccagccggcc gcgttgccgt tctggatcgt caggccggag aaggtcacaa
      121 cgctcgtgcc gatgatctga aacacctgat cgagctgccc gccatcgacg atcgtggaca
      181 ctgcgcccgc cccctggatg agcaggtcgc cggcaatatc gaggtcgccg gtcgcggcga
      241 ggtcctcgaa cgtgccggag atcgtcagcg catagagccc ggccgggacg atgatggtgt
      301 ccgcgccggc cagcgcgttc gcttcctgga tggcagcgcg gagggtacag ctatcgtcgc
      361 cgatgtcgca gagcccatcg ccgggcgcgc tatcgaccgc gtcgatcgtg ttggtgacgg
      421 tgaatgtcgc cgagggctgg gcgagtgacg gacggctcca gacgaccaac gagccgcagg
      481 ccagaacaac gatcaggacg atcgcgagcg cacgcgtacg catgattcgg ctcccccggc
      541 cgagcgttgg tctcctctat gtggcgccag cactggccct gaacgcgtcg aagggacatt
      601 cgtggggcag acaagcgacg ttccacaaca cgggtgaatg cggaacgtgt cgtcgccgga
      661 cctctgccag agtagccgtt cctaccctac tctacggcgt gcattcagtg acaaagtggt
      721 tgagatcgat cagaacgcgg cttctggtgt caatgacgta ccgtgcgagc gcagatattc
      781 gggagcgata tcggcgccgt taggccagac gacggttcct agctcttggt cgacgtacgc
      841 cagtgcgaag accgctggat cgcgcaatgg ctcgaacact ggtccccaca ggctatcttc
      901 caggtcaacc tggcggattt ctccgttgcg gaacgtgaca tccagcacat acggcccgac
      961 taccgtaacc ttggttacta cctgcatgcc ggctaccttg ccatcctatg gcagcggtgg
     1021 aatccgctcc agcggatcat gtctccgcgc acgttcccag tttcgcagga ggtcgtgctc
     1081 atggagtgct atccactcta gcaaaaaccg ctccgcgcgg atcgatacga tcctgttcat
     1141 ccataatcgc cggtggagag atggtgactc tggtccgctt gccgccatgg gcagcatgta
     1201 catgcggagg atcatgatcc agatagtaca tggcgacgac gattccccga aatcgagaca
     1261 attcgggcaa ggttgttgcc ttcactgatt cgatgaatgg atcgagcgtc atcgtcccga
     1321 agctcacgga ataatccggt ggcatcacat gacactgcag gccagcaaca cgatatcgaa
     1381 acgaagcgaa ctgtgccgat aggaatcctc atgcaaagat ggacgatctg ccgacgatga
     1441 cggccgggtg gagcggggcg gcgttcatat tgtcgatgat gacggacagg ccgatgaaca
     1501 ggctggcggc gatggcggcg gcgaggacga cgaagagcag gcgttccttg aacatcatgg
     1561 ttgattcccc ttctggtgtg acgtgcttca acaacacgac cagcatagcc agtcggggag
     1621 atagcgcctg ccagcgctga cacccacttg ctgtgttccg cgcaacacac cggccggctc
     1681 cgtcatggag ccggccggtt ccctgcggtc gaggattgcg acctagttcg tgctcatcgc
     1741 agcaccacca ccgcgcgggt cggcgccgcc gcgcagtgtg ccggtcgccg ggtcgcgggt
     1801 gatggcctgg acgagcgcga agctgccgta cggggccgag ttcggccaga tcgtgaagcc
     1861 gcgctcggcc agcgccgcct tcgtccagct ggggatgcgc gcttcgcagt cgagcgtgtc
     1921 gctctggcag tcgaagcgcg gggccgagac ggcttcgacc ggcgataggc cgtgatcgag
     1981 gacattcagc agcgtctgga ggacgccggt gatgatccgc gtcccgcccg gcgcgccgag
     2041 ggcaagcacg acctgtccat cgcggaggac gatcgttggg cacatcccgg tgatgcgtga
     2101 cttgcccggc gcgatcgagt tgggcagacc cggctgcggg ttggcggcgt tcatgatgtt
     2161 gttccaggtg aagccgtagc cggccggaac gacgccgctg ctggagccga gcgagtgggt
     2221 cagtgcgatc gcgttacccg catcgtccac gacggtgatg ttggtcgtcg tcggagcctc
     2281 ggggtagcgt gggacgctga attgctcacc agccttgatc cgcgcgtacc acttgtcggc
     2341 gtgctcgtcg gagagcagca tcccaaccgg gacatcgacg aaggcggggt cggcgacgtg
     2401 cgcataccag tcgtggtagg cagccttcat tgcgtgcccg acgaggtcga tgtactcgac
     2461 actgttgaga ccgagggcgg cgatgtcttc gtgctcgatg atcttgagcg tctgcgccat
     2521 gcagatgccg ccgccagccg gctggttggt caggatcgtc tggccgcgat agtcgatccg
     2581 gatcggctcg ctgacccgga ccttgtagct ggccaggtcg tcggcagtga tgaacgagcc
     2641 gttggctgcc aggtcggccg ccagcttctt cgcaagatcg ccctcgtaga actcgcgcgg
     2701 gccgccatcg gccagccgct ggagtgtacg cgtgtggtcg gggttcatga tgcgctcgcc
     2761 gggcttgtag ccggagccgt cgggcttgaa gtagattgcg cggctcggcg cggtcgcctg
     2821 caaccgatca ccgaacgaga tatgcaaccc gtagccgggc gtgttccaga ttcgccacag
     2881 ctccggcgtc acgacgtagc cggcgtcgga gatgcggatc gccgcctgga gggcatcctt
     2941 ccacgagatc gtgccgaagc ggtcgagcag ctcggccatt ccagcgacgg tgcctggcgt
     3001 catgatcgaa ccgtagccga cgtcgttgac cttgccttcg aggtggtagc cgtagccggt
     3061 ccagtcctgc tcgatgacga tgtcctgcca catctcgggc gtcgccttcg agccggccgt
     3121 tccgttgaag tcgatcgtca cttcctcacc ggcggcggtc cggacggtgg ccgcgccgaa
     3181 accaccgatc ccgcacatct gcgggtcgac gaccatctga acgaaggcgg ccgtaacggc
     3241 cgcgtcgacg gcattgccac cgttgcgcag gacatcgaca ccagtctcga ccgccggcac
     3301 ctgtggcgcc acgaccatcc cacccatgac cgtctcctct cgctgttgcc tgaactcgcg
     3361 ggatcgcgtc tgcggatcat cgccggatgc gcgtcgttcg gcaagtccca cgctactgat
     3421 gcgtcgaaac gcacaagagc acacgcacgc caggggactc gcctgtatga ttcgctacct
     3481 tcaattcgaa cgacgaagaa tcggggcaca atgcaccgga ttgccagcct gctgctgatc
     3541 gcgcttctcc tggtcgcctg tggcggcggc gatgacgacg cgaacgattc gtcgtctaac
     3601 tcggcaccga ccgcgacgat cgcaagcagc acgaccgtcg ccggatcgac ggcggcgcct
     3661 gcccagccga cgacgcaggg ggcagcggca acaatgccga ctgagagcgt ctcaacgcct
     3721 acggtttcga cgggtggggg cggtgaggat gtcgaaggtg ctccgaccaa tgccagcgcc
     3781 gatttccccg acgtgcgcgt gccgctggaa gcgggcagca gcggcgaggt cggctcgatg
     3841 gaccccgatc cgttcgctgc gactgacacg ctcgacgaga actcgaaggt gaaggtcacg
     3901 atcaacgagg tgctcgaccc ggcgaccgtc gatggaacga tcttctcgcc ggaggagggt
     3961 aatcgctggt tcgcgatcaa cgtcacgatg gaggcgaccg gcagcgaggt cgccaacacc
     4021 ggtgagtgga cactgtccac gaccgacggc gccgaataca ccaactcgat cgtcctcggc
     4081 agcacaccag acatcaccta tggcagcatc gaagccagca catcaaccga aggaatagtc
     4141 gtcttcgaga ttcctgaggg cgccgaggtc gcctggattc tgatgagccc gacgatcttc
     4201 gtcggcggaa acctggtctt cgtgaactga ccagagaaaa ccccggcggg tggaagcctg
     4261 ccggggtcga cacattggat agacaacccg cctatcctct gcgtgcctgt caagtgtgcc
     4321 tgatggcgtg cgtgctgtca cctggccggt gcgggatcga tcag
//
LOCUS       DASUGJ010000292         5770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_598617, whole genome shotgun sequence.
ACCESSION   DASUGJ010000292 DASUGJ010000000
VERSION     DASUGJ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598617"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VFV93_06490"
     CDS             complement(<1..913)
                     /locus_tag="VFV93_06490"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5165027.1"
                     /translation="MNTATRVSAIITTYNYGEFIVEAIESVLNQTRPPDEVFVVDDGS
                     TDQTSELVAPYVERGQVVYIQQANSGPSEARNNGFERSTGDLIAFLDADDTWLPDKLE
                     RQLAWLAAHPRASMVSGPMIWWHVPRDERWVVAFKAMPAARFRREVTIRNLVGNPSMA
                     LIRRTALERAGLFDTTLRWGQDWEIFVRLARVGEVGFVADPVIVYRWHRGNLSHERRM
                     EQLSMNHSISRRAIATHLPGWQRPILRLRSWSVIEFDRARIHHTEGGPRVRTFRHAAL
                     ALLSWPLEDTREKAKLLGRSLVGEATYQ"
     gene            complement(934..2403)
                     /locus_tag="VFV93_06495"
     CDS             complement(934..2403)
                     /locus_tag="VFV93_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX5165028.1"
                     /translation="MTAASTTQRADLSKQAVQSTVWAYGSFVSGKLLVFVSTIILARL
                     LLPSEFGLMGYCLIGMQYLDILNGFGVGHALIARRDKVEEAANAALIIGLVSGMLLFA
                     VSWFGAPYLAAFFDEERITTLFRALAIVIPISAVGAVPSSLIQRSLRFKAKFVPDIGR
                     SIAKGVISVVLAWQGYGVWSLVIGQIAGEVVATAVFFALAGWRPTRLFDRQVTREVMG
                     FGSHIISIGIAGAVISNFDYLLVGKVLGATALGFYTFAYRIPDLIIRNTNVVIGKVAF
                     PLLAQVQSDAEELRLAYGTMLRYVSLFTIPAGIGMALIAPRFVSAFFPDRWEPATGVM
                     QLIALALAIASVGHLPGIIYKAVNRPEILNWTSFLKLAVTAGVLWYCVRWDITGVAAG
                     QVGLAVFFVVIDMVVVWRVLDFGGRALLRAIAPSLLCTLAMTIVVVLLNRTMPAGGIA
                     GLMAIVAVAVVVFIVTLATVSAETVASARNVLRSALSRA"
     gene            complement(2400..3560)
                     /locus_tag="VFV93_06500"
     CDS             complement(2400..3560)
                     /locus_tag="VFV93_06500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5165029.1"
                     /translation="MTDRITVLTVIDSLGVGGAQALLPTFLRHVDRERFDIRILALTA
                     APSNHIQDAILAEGAPLEQWAGNGLLKMRRIRALANEIRRQQVDLVHSHLLYSNVQAG
                     IAARLTHRPLVATLHNVQQYSPYLKRTIEAGVLRATRARALAVSDGVRRSFRGRPGLS
                     ERQLAVVPNAIELQRFTDLTPESVALARQEALAGAPGPLIVAVGRLTPQKGFAILAEA
                     AAIVRTQCPDARFAIAGREADGAAELRAAIQRHNLADVVQLMGQRSDVAQLLAAADIY
                     VSPSLSEGAPVTHLEAMAAGAPVVATRVGGVPEIIRDGDNGLLVPAGQPVPLADALLR
                     LLGDPVLARRMANLGRASVQEFGAEAWARRIECEYLRALGCHVSECREEMRR"
     gene            complement(3553..>5770)
                     /locus_tag="VFV93_06505"
     CDS             complement(3553..>5770)
                     /locus_tag="VFV93_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5165030.1"
                     /translation="FALMMYSRASEVLTTSLGIPSIAPLFTIWLLTGIVMHYGLGGVF
                     HARPTGWQALAVYGLVLLASALVAADAWRSTYRVVEYTRELVYIALIVTCVRQLSDLR
                     IVWRALLIGGTIPALITIYQTISGSTNWFLGFGRYSAQIVVPGEIVEVPRPAGMVGDP
                     NMYAMALIALIPLAFLGARWEKHWLARTLWLLSAVALMLASIMTYSRGGYVSLAVVIA
                     CLAVTGFIKFRSLLMVGLIVLVLMPVLPDSYTSRISSLTSIPMSLVSDEPPKPGEATD
                     SSVDGRVAEMMAGLHMFMDHPILGVGTNNYQVHFQEYARPLGINRRADRAAHSLYVEI
                     AAETGIVGLLSFGALVVTLFVSLRRVWTTNNKGNELHDLALIVAISLISLLVSCIFLH
                     FAYPRFFMVFAGLAMAVASVQNWRERPASRLRFARLQPSQRLLTPAQHGRAVVWGGAG
                     AIGSVALVAAIMMTMVVKPGGLTLLPEDKAAAIGPQIAGAPAPGNDGTSQSVPPVAGQ
                     TPTAGNVEPTAVPPTKTPAPEPTPEVAIATQADLLRAALAPVGTRPDCRYDADAKHNI
                     CDHFLEFYKANGGEAAFGRPLSEQYIIAGTLVQYYERARFEWHAGENGQADSVLLSRL
                     GAQQRLRTTGHEIDPTALPEEDEGCIYEDVTGHNVCGEFAFYWITNGGASLLGYPISE
                     RTIANGVSTQYFERARLEIHLSAAGTPEVVAANLGTEEVQRILTEAGADDGTGPGD"
BASE COUNT         1111 a         1931 c         1860 g          868 t
ORIGIN      
        1 attggtacgt cgcctcgccg accagcgagc ggccgaggag cttcgccttc tcgcgggtat
       61 cctccagcgg ccacgacagc agcgcgaggg cggcgtggcg gaacgtcctc acccgcggtc
      121 cgccttcggt gtggtggata cgcgcacgat cgaactcgat cacgctccac gagcgcaaac
      181 gcagaatcgg gcgctgccag cctggcaggt gagtcgcaat cgcccggcgc gagatcgagt
      241 gattcatcga gagctgctcc atccgccgct cgtgcgacag gttcccccga tgccagcggt
      301 aaacgatgac cggatcagca acgaaaccga cctcaccgac gcgcgccagc cgaacgaaga
      361 tctcccagtc ctgcccccag cgcagggtcg tgtcgaacag accggcccgt tcgagcgccg
      421 tcctgcgaat gagcgccatc gacgggttgc cgaccaggtt acgaatcgtc acctcgcgcc
      481 ggaagcgggc ggcaggcatc gccttgaacg ctacgaccca acgctcatca cgcgggacat
      541 gccaccagat catcggcccg ctgaccatcg acgcgcgtgg gtgcgccgcc agccaggcca
      601 gctgtcgctc cagcttgtcg ggaagccagg tgtcgtccgc gtcgagaaag gcaatcagat
      661 cgccggtcga gcgctcaaac ccattgttgc gcgcttcact tggaccgctg ttcgcctgct
      721 ggatgtagac gacctgtccg cgctcgacgt atggcgctac cagttcgctc gtctgatcgg
      781 tcgagccgtc gtcaacgacg aagacctcgt ccggcggccg cgtctggttg aggacgctct
      841 caatcgcttc gacgatgaac tcgccgtagt tgtaggttgt gatgatggcg ctgactctgg
      901 tcgctgtgtt catcacaggt cactccggct ccgctacgcg cgcgacaacg ccgaacgcag
      961 gacatttcgg gcactggcaa ccgtctcggc cgagacagtt gcaagggtaa cgatgaagac
     1021 aacgacggca accgccacga tcgccatcag gcccgcgata ccaccggccg gcatcgttcg
     1081 gttcagcagg acgacaacga tcgtcattgc cagcgtacag agcagcgaag gggcgatcgc
     1141 tcgcagcagc gcgcggccgc cgaagtcgag cacgcgccag acgacaacca tatcaatcac
     1201 gacgaagaag accgccagcc caacctgccc agccgcgacg ccggtgatgt cccagcgcac
     1261 gcaataccag aggacgccgg ccgttaccgc cagcttcagg aacgaggtcc agttgaggat
     1321 ctccggccgg ttgactgcct tgtagatgat acccggcagg tggccgaccg acgcgatcgc
     1381 cagtgccagc gcgatcaact gcatcacgcc cgtggccggt tcccagcgat ccgggaagaa
     1441 cgcgctgacg aagcgcggcg cgatcaaggc catcccgatt ccagccggaa tcgtgaagag
     1501 cgagacatag cgcaacatcg tcccatacgc cagccgcagc tcttcggcgt ctgactggac
     1561 ctgcgcgagc aatgggaagg caaccttgcc gatgacgacg ttcgtgttgc gaatgatcag
     1621 gtccgggatg cggtaggcga atgtgtagaa gcccagcgcc gtcgcgccga gaaccttgcc
     1681 aaccagcagg tagtcgaagt tcgagataac ggccccggcg atcccgattg agatgatgtg
     1741 cgacccgaaa cccatcacct cgcgggttac ctgccggtcg aacaggcgcg tcggacgcca
     1801 gccggccagc gcgaagaaga ccgccgtcgc gacgacctcg ccggcaatct gcccgatgac
     1861 gaggctccag acgccgtagc cctgccaggc aagcacgacc gagatgacgc ccttggcgat
     1921 gctgcgcccg atatccggca cgaacttcgc cttgaagcgc aagctgcgct ggatcagcga
     1981 ggacggcacg gcgccgacgg cgctgatcgg gatcacgatc gccagcgcgc ggaacagcgt
     2041 tgtgatccgc tcttcgtcaa agaacgcggc cagatagggc gctccaaacc acgagacggc
     2101 gaacagcagc atccccgaca caagcccgat gatcagcgcc gcgttggcgg cctcttcgac
     2161 cttgtcgcgg cgggcgatca gcgcgtgacc gacgccgaac ccattcagaa tgtcgaggta
     2221 ctgcatgccg atcaggcagt agcccatcaa cccgaactcg gacggcaaca gcaagcgcgc
     2281 caggatgatc gtgctgacga agacgagcag cttgccggag acgaacgagc cgtaggccca
     2341 gaccgtgctc tggaccgcct gcttcgagag gtcggcgcgt tgcgtggtcg atgcagccgt
     2401 catcggcgca tctcctcgcg acactcactg acgtgacacc ccagcgctcg caggtactcg
     2461 cattcgatcc tgcgtgccca cgcttcagca ccaaactcct ggacggaggc gcgaccgagg
     2521 ttcgccatcc ggcgcgccag cactggatca ccgagcaggc gcagcagtgc gtcggctagc
     2581 ggcaccggct gaccagccgg aaccagcagg ccgttatcgc cgtcgcggat gatctccggc
     2641 acgccgccaa cgcgggtcgc gacgaccggc gcgccagccg ccatcgcttc caggtgcgtc
     2701 accggcgccc cttcgctcag cgacggcgag acgtagatgt cggcggccgc cagcagttgg
     2761 gcaacgtcac tgcgctgccc catcagttga acgacatcag caaggttgtg gcgctgaatc
     2821 gccgctctca gttcggcggc tccatccgcc tcgcggccgg cgatcgcgaa gcgtgcatcg
     2881 ggacactgcg tccggacgat cgcggcagcc tcggcgagga tcgcgaagcc cttttggggc
     2941 gtaagccggc caacggcaac gatcaacggc cccggcgcac cggccagcgc ctcttgccgg
     3001 gccagcgcga ccgactcagg cgtcagatcg gtgaaacgct ggagctcgat cgcgttgggg
     3061 acgaccgcca gttgccgttc agatagcccc ggtcggccac ggaacgagcg acgcacgccg
     3121 tccgacacgg ccagcgccct tgcacgtgtc gcccgcagga caccggcctc gatcgtccgt
     3181 ttcaggtagg ggctgtactg ctggacgttg tgcagcgtcg ccaccagcgg gcggtgggtg
     3241 aggcgggcgg cgatgcccgc ctggacgttg ctgtagagca ggtgcgaatg gacgagatca
     3301 acctgctgcc ggcggatctc gttcgcgagc gcgcggatac ggcgcatctt gagcagaccg
     3361 tttccggccc actgctccag tggcgcgccc tcggcaagaa tcgcatcctg aatgtggttg
     3421 gaaggcgcag ccgtcaacgc caggatgcga atatcgaatc gctcgcggtc gacgtgtcgc
     3481 aggaaggtcg gcaacaacgc ctgggcgccg ccgacgccga ggctgtcgat cacggtcagg
     3541 acggtgatgc gatcagtcac cggggcccgt cccgtcatca gcgccggctt ccgtcaggat
     3601 gcgctgcacc tcctcggtgc cgaggttcgc ggccaccact tccggcgtgc cggcagccga
     3661 caaatggatc tccagacgcg cccgttcgaa gtattgggtg ctgacaccgt tcgcgattgt
     3721 ccgttcggag attggatagc cgagcagcga tgcgccgcca ttggtgatcc agtagaaggc
     3781 gaactcaccg cagacgttgt ggccggtcac gtcctcgtag atgcagcctt cgtcctcctc
     3841 cggcagcgcc gtcggatcaa tctcgtggcc ggtcgtccgc aagcgctgct gggcgccgag
     3901 ccgcgagagc aggacagagt ccgcctggcc gttctcacct gcatgccact cgaaacgggc
     3961 acgctcgtag tactgaacga gcgtcccggc gatgatgtac tgctccgata gcggacggcc
     4021 gaaggccgct tcaccgccgt tagccttgta aaactctaga aaatggtcgc agatgttgtg
     4081 tttcgcatct gcgtcgtagc gacagtcagg ccgcgtcccg accggcgcca gcgccgcgcg
     4141 cagcaggtcg gcctgggtag cgatcgcgac ttccggcgtt ggctccggtg ccggcgtctt
     4201 cgtcggcggg accgcagttg gctcgacgtt gccggccgtt ggcgtctggc cggcgacggg
     4261 cggaacggac tggctggtcc cgtcgttgcc gggagcaggc gcaccggcga tctgcggccc
     4321 gatggcggcg gccttgtcct ccggtagcag cgtcaaccct ccgggcttga cgaccattgt
     4381 catcatgatt gcggcaacca gcgcgaccga gccgatcgct ccggccccac cccagacgac
     4441 cgcccggccg tgctgggcgg gggtcagcag ccgctgggac ggctgcaagc gggcaaagcg
     4501 cagccgcgac gccggccgct cacgccaatt ctggacactg gcaaccgcca tcgccagccc
     4561 ggcgaaaacc atgaagaacc gtggataggc gaaatgcaga aagatgcagg aaacgagcag
     4621 cgagatcagc gagatcgcca cgatcagcgc caggtcgtgc agctcgttgc ccttgttgtt
     4681 ggtcgtccag acccggcgca gcgagacgaa cagcgtcacg accagcgccc cgaacgacag
     4741 caggccgacg atgccggttt cggcggcgat ctcgacgtag aggctgtggg cggcccgatc
     4801 ggcccgacgg ttgatcccca gcgggcgggc gtactcctgg aagtggacct ggtagttgtt
     4861 ggtgccaaca ccgagaatcg ggtggtccat gaacatatgc agcccggcca tcatctcagc
     4921 gacccgacca tcgaccgagg aatcggtcgc ctcgcccggc ttgggcggct catcggacac
     4981 caggctcatc gggatcgatg tcagactcga gatgcggctc gtgtaactgt ccggcaggac
     5041 cggcatcaag accagcacga tcagcccgac catcaacagg ctgcggaatt tgatgaaccc
     5101 cgtcaccgcc aggcaggcga tgacgaccgc cagcgagaca tagccgccgc gtgagtaggt
     5161 catgatcgac gccagcatca gcgccacagc actcagcagc cagagcgtcc gcgccagcca
     5221 gtgcttctcc cagcgtgcgc cgaggaaggc gagtgggatc agcgcaatca gcgccatcgc
     5281 gtacatgttg ggatctccga ccatcccggc cgggcgggga acctcaacaa tctcaccggg
     5341 aacgacgatc tgggccgaat agcgcccgaa gccgaggaac cagttggtcg atcctgagat
     5401 cgtctggtag atcgtgatca gcgccggaat cgtcccgccg atcagcagcg cccgccagac
     5461 gatacgcaga tctgaaagct ggcgaacgca ggtgacgatc agcgcgatgt acaccagctc
     5521 acgggtgtat tcgacgacgc ggtaggtgga ccgccaggcg tcggccgcca ccagcgccga
     5581 ggccagcagc accagaccgt agacggccag cgcctgccag ccggtcggcc gggcgtggaa
     5641 gacgccacca agcccgtagt gcatgacgat gccggtcagc agccagatcg tgaacaacgg
     5701 cgcgatcgac gggatgccga gcgaggtcgt caggacctcc gacgcgcgcg agtacatcat
     5761 caacgcgaag
//
LOCUS       DASUGJ010000293         3026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_739095, whole genome shotgun sequence.
ACCESSION   DASUGJ010000293 DASUGJ010000000
VERSION     DASUGJ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739095"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VFV93_06510"
     CDS             <1..1179
                     /locus_tag="VFV93_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165031.1"
                     /translation="KPEAAPKVKPDHRVDGSWLEKGWYDHINIVDGLLGWPWQEPLPE
                     IDINPLDDPRPEEVDGWLTEWAGWIKEQYPGAPVDPGAVTIYLDAAHTPESTPSQAYA
                     YAVFGEGGAYAPIVLTPAGRSLDQQVALDKGAVAGVDRGVAQRLEALGVTSVDALSSS
                     WSGLVAGALGIDDGAATALIADARAQIPALQSSLLGFSGVDSQLDATLKGTAFNTPVA
                     LANARPDDLVQAVVQQGTMTEAFAERLISEARRTVPADQWSLTSSQLNLKQEEITRLN
                     QLGVTTLKQFTDLAATQAGAGSIAGVLNVTPAQVTSLSDQVTIKDAAALATDRIAVGA
                     VTGVLGVNSETGRSLAGLGVATVGALATADPDALAAAFGGQRDSALAAINNARLRLGN
                     "
     gene            1181..>3026
                     /locus_tag="VFV93_06515"
     CDS             1181..>3026
                     /locus_tag="VFV93_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165032.1"
                     /translation="MGTSSFADVEIGQVRLRCRGEVAGETALRLRVDHLLGSADIQPV
                     GMSPSAVLIVRRLADPLPGRLAPHEPRVRVDRSWEQATRSALASVWQAAARPVAGVVP
                     AGAEAILFDDEASLLALLAADVCAGRANERWWWQALPRSLVPPPGRLDQLLLRQPRLI
                     PAIIHQLAAFGELGPVVAALTPRQAGSIVVAMLSAYGMTIPTSRPTEDHLERASQSAA
                     DVGPIWDAWTPPHLDMPDAGREHQCLVGLGLGLFQAPAMIRGAAFQQRFHTWWSASAG
                     RDDPSSAATAPEVVSDASAERTRTADMPRTAIPSSEGESSQPSGAQHVTVASGSGDST
                     RQPELVDVTALEGPGGAIHPVETSPISRLKENDITTNNLDPDPVENDSGTVAEAFGAG
                     IDAEAVRDDILDLDQPELDTSVVTTTRDAEPFGLAFAGGFRTELGGFAYLINLMCRLD
                     LPDCFEDDWRLATAVGPWGTLELLARALAGSSVADDALWSALAALAGRPEGTPPGLEL
                     VQPTLTRLPETWLPADAASGEWQPSPLTDGLGEAAVEWLRLVMPVVRYFLSLRLEGDD
                     PGAELLRQPGQVYISATHVDVVLPLDAISIPIRIAGLDRDPGWLPDFGR"
BASE COUNT          491 a          997 c         1053 g          485 t
ORIGIN      
        1 aagccggagg cagcgccgaa ggtgaagcct gaccaccgcg tcgacggttc ctggctcgaa
       61 aagggctggt acgaccacat caatatcgtc gatgggctgc tgggctggcc gtggcaggag
      121 ccgctcccag aaatagacat caacccgctc gacgatccgc gtccggagga ggtcgacggc
      181 tggctgaccg agtgggccgg ctggatcaaa gagcagtatc ccggggcacc ggtcgatcct
      241 ggggcggtca cgatctatct cgacgccgcc cacacgccgg agtcgacgcc gtcccaagcc
      301 tacgcctacg ccgtcttcgg cgagggcggc gcctatgcgc cgatcgtcct gacgccggcc
      361 ggccggtcgc tggaccagca ggtcgcactc gacaagggcg ccgtcgctgg cgtcgatcgc
      421 ggcgttgccc aacggctgga agcgctcggt gttaccagtg tcgatgcgct ctcgtcgagc
      481 tggagcgggc tggtggccgg tgcgctgggg atcgacgacg gggcggcaac ggcgttgatc
      541 gccgacgccc gcgcgcagat cccggccctc cagagcagcc tgctgggctt cagcggcgtt
      601 gatagccagc tcgacgcgac gctgaagggg acggctttca acacgccggt cgcgctcgcc
      661 aacgccaggc cggacgacct cgttcaggca gtggtccagc aggggacgat gacggaggca
      721 tttgccgagc ggctgatctc cgaagcgcgc cggaccgtcc cagccgacca gtggtcgctg
      781 acctcgtcgc agctcaatct caagcaggag gagatcaccc gcctgaacca gctcggtgtg
      841 acgacgctca agcaattcac cgacctggcc gcaacgcagg ctggggccgg ctcgattgcc
      901 ggtgtcctca acgtgacacc ggcccaggtg accagcctga gcgatcaggt cacgatcaag
      961 gacgccgccg cgctggcaac cgaccggatc gcggtcgggg ccgtcacggg cgtgctgggc
     1021 gtcaacagcg agacgggccg ctcgctggcc ggcctcggcg tcgcaaccgt cggcgcgctg
     1081 gccactgccg acccagacgc gttggcggcg gccttcggcg gtcaacggga cagcgcgctg
     1141 gcggccatca acaacgccag actccggctc ggcaactgac atgggaacat cgtcgttcgc
     1201 cgacgttgaa atcggacagg tcaggctgcg ctgccggggc gaggtggccg gcgagacggc
     1261 gctgcgcttg cgggtcgatc acctgctggg tagcgccgat atccaaccgg tcgggatgtc
     1321 accgtcggcc gtgctgatcg tgcgccggct ggccgacccg ctgccgggca ggctggcgcc
     1381 ccacgaacca cgcgtgcgcg tggatcgcag ctgggagcag gcgacgcgct cggccctcgc
     1441 tagcgtctgg caggccgccg cgcggcccgt agcgggtgtc gtcccggccg gtgcggaagc
     1501 gatcctgttc gatgacgaag ccagcttact ggcgctcctt gcggccgatg tctgtgcggg
     1561 acgcgccaac gaacgctggt ggtggcaggc gttgccgcgc tcgctcgtgc cgccgcccgg
     1621 caggctggac cagctcctcc tgcgccagcc gcgactcatt ccggcgatca tccaccagct
     1681 cgcggcgttc ggcgagctgg gacctgtcgt ggctgccctc actccccgcc aggcaggctc
     1741 gatcgtcgtc gcgatgctgt cggcatatgg catgacgatt ccaacctccc gcccaaccga
     1801 agatcatctg gaacgggcga gccaatcggc agcggacgtc gggccgatct gggatgcctg
     1861 gacgccgccg cacctcgaca tgcctgacgc aggccgggag catcagtgtc tggtcgggtt
     1921 ggggctgggg ctgttccagg caccggcaat gatccgcggc gcggcgttcc agcaacggtt
     1981 tcacacgtgg tggtcggcat cggctggtcg cgacgatcca tcctccgctg ccaccgcacc
     2041 cgaagtggtc agtgacgcat cggcggagcg tacccgaaca gcggacatgc cccgaaccgc
     2101 aattccgagc agcgaaggag agtcgagcca accgagcggc gcgcagcatg tcactgtcgc
     2161 ctcaggtagc ggggattcga cacggcagcc ggaactcgtc gatgtgacag cgctcgaagg
     2221 accgggcggg gcgattcacc cggttgagac atcccccata tctcgactca aagagaacga
     2281 cataacaacg aacaatctcg atcctgaccc agtagagaac gactccggca cggtggcaga
     2341 agcgttcggt gcaggcatcg atgctgaggc agtgcgagac gacatcctcg atctcgatca
     2401 gccggagctg gacacgtccg ttgtgacgac cacgcgtgat gccgagccct tcggactggc
     2461 gttcgctggc gggttccgga ccgagttggg tgggttcgcg tatctgatca acctgatgtg
     2521 ccggctcgat ctaccggact gtttcgagga tgactggcgg ctggcgacgg cggttggtcc
     2581 atggggaacg ctggaacttc tggcgcgggc gttggccggt tcatcggtag ccgatgacgc
     2641 gctctggtcc gcgctggctg cgcttgccgg acggccagaa gggacgccgc cggggctgga
     2701 actcgtccag ccgacgctca cacgcctgcc ggagacatgg ctgcctgctg acgcagcatc
     2761 cggggagtgg cagccgtctc cgctgacgga tggactgggc gaggctgcag tcgagtggtt
     2821 gcggctcgtc atgccggtcg tgcggtactt cctgagtctc cggctggagg gtgatgatcc
     2881 cggagcggag ctattgcgcc agccggggca ggtgtatatt tcggccaccc acgtcgatgt
     2941 cgtactgccg ctcgatgcta tctcgattcc catccgcatc gctggtctgg atcgcgatcc
     3001 cggctggctc cccgacttcg gccgcg
//
LOCUS       DASUGJ010000294         4706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_322929, whole genome shotgun sequence.
ACCESSION   DASUGJ010000294 DASUGJ010000000
VERSION     DASUGJ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322929"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFV93_06520"
     CDS             complement(<1..435)
                     /locus_tag="VFV93_06520"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX5165033.1"
                     /translation="MIDTGELRKGIIIEYEGTLQRIIEYQHVKQGRGSAFVRLTMRNL
                     RTGSTTQQTFQAGSKFPLVRLERQRAQYLYAEDESYNFMDLDTFEQITLDKETLGDAL
                     NYIKEQDTIDLLTYQSEPIDIEIPITVNLAVTRTDPGLRGDTA"
     gene            complement(432..1562)
                     /locus_tag="VFV93_06525"
     CDS             complement(432..1562)
                     /locus_tag="VFV93_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5165034.1"
                     /translation="MSESSQRVERVRALMAEKQLDCLLITHPSNRVYLTGFTGDDIAP
                     NESSGHLFITATDAVLVTGGVNVTQAEAQAPHVRVIKREGGWPQADAAVLTDLGVHKI
                     GYEAQAMLVGVFHGISEQLNEKKHDHEWVVADGLVEELRIVKSESEIALLRKAFQITN
                     EAFDRVAPTIQAGQTEAEVAWKIHQAFVELGAEGPSFPTIVAAGTNAARPHHEPGERV
                     IQEGEPIVIDMGARYHGYCADLTRTVWVGEPDEKLREVYPIVAAAVEEVIERSQIGTT
                     GADMDAAARQYIDARGYGEAFNHGLGHGVGVRVHEGPSASKESKDVLAAGHVITVEPG
                     IYLPEWGGVRVEDVILITESGIEVLTSASKMRIENGDGSKRS"
     gene            complement(1555..2034)
                     /gene="aroQ"
                     /locus_tag="VFV93_06530"
     CDS             complement(1555..2034)
                     /gene="aroQ"
                     /locus_tag="VFV93_06530"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX5165035.1"
                     /translation="MSESALDGRTVLVIHGPNLNLLGRRQPEIYGTTTLDEIDEALQQ
                     AADERGFQLVVLQSNYEGALIDQIQRYGWVASGIIINPGALTHYSIALRDALAAVPAP
                     AIEVHLSNIAAREEFRHHSVISAIAHGTIAGLGASGYVLALHYLMDLVELKEAQAGE"
     gene            complement(2031..3665)
                     /locus_tag="VFV93_06535"
     CDS             complement(2031..3665)
                     /locus_tag="VFV93_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX5165036.1"
                     /translation="MMQRRAAIPIVAGLVLLSVFSALAIRNGDNVADASAVLRWGYYV
                     NYDEASRASLDANLSHLDVVSPYWYHITPSGETKSFAQPTVTAMLQANGVKVLPLVQT
                     EPQWDEFHKTFETPEKRDAIVARLVTVVESNGYDGIQIDFEGINASDDDILTDFMKRL
                     HAAFQPRGWIVSQAVIARTSDAANYWGGAYDYSELAKYNDYIAIMAYDYGYAGRPDPI
                     AVAPIWWVDDVAKYAVSQIPREKIILGVPFYGYDWNTTKGPPATSVSYDSAMKLAERK
                     DADLTYDKTNAANRLRYKDDAGDNHEVWFENADSFDAKLKIVTDYRLAGFAGWRLGHE
                     DPATWTVIASLQTPATRIPPFEQTSDRIYFPETGHSLAYGFLDYWLANGGLARFGYPR
                     TEEFTETDPMVGISYTVQYFERARFEYHPEFAGTEFAVLIGHTGRWALAQRGMDPWET
                     ATGPKSGARYFEETGHNLGGAFQRYWEANGGLMTFGYPITEEFTEVNPEDGQSYVVQY
                     FERARFEYHPEYAGTDSEVLLGLLGNEMLRERGWIR"
     gene            complement(3670..>4706)
                     /locus_tag="VFV93_06540"
     CDS             complement(3670..>4706)
                     /locus_tag="VFV93_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165037.1"
                     /translation="PPTSTTPPDTATTSRPTATTEQVAPRSPTEDIATDEPTPTEEVA
                     EIEPTATTVIEEPEPTEEPPAIGDFGTLPPAQIVSGGLSRTLDLDYELAVSLSDSPSS
                     APVFLLEWPAWTEDDVATIAGNLGLEGAVEGGPGNFQVIGATSEIYFNGSTVQYVYTS
                     SLPDLPLGTDANVIESARSWIYANGFISDDLNGGAVIGRDDDAGRAVVLFKPAQVSPV
                     LSFVPSATVTVGPGGTVVEANIRWPAGYVDSEYGLWSGDALWNKVLAGEASVEADLSS
                     VGGSGALSGTMTVSGITLAYSYAGSPGGDEYLVPLIVFSGEATINETGDVVPVSVYVA
                     AIAGQATPQG"
BASE COUNT          744 a         1448 c         1600 g          914 t
ORIGIN      
        1 cgcggtgtcg ccgcgcagac cgggatcggt cctcgtgacg gccaggttga cggtgatcgg
       61 gatctcgatg tcgatcggct cgctctgata ggtcagcagg tcgatcgtgt cctgctcctt
      121 gatgtagttg agtgcgtcgc cgagcgtctc tttgtcgagt gtgatctgct cgaaggtgtc
      181 gaggtccatg aagttgtagg actcgtcttc ggcatacagg tactgtgctc gctgccgttc
      241 gagtctgacg agcgggaact tgctgccggc ctggaacgtc tgctgggtcg tcgacccggt
      301 cctcaggttg cgcatcgtca gccggacgaa ggcgctgccg cggccctgct tgacatgctg
      361 gtactcgatg atgcgctgca aggtgccttc gtattcgatg atgatgccct tgcgcagttc
      421 gccggtgtcg atcatgaacg cttgctcccg tccccgttct cgattcgcat cttggatgcg
      481 cttgtcagaa cttcaatgcc gctctcggtg atcagaataa cgtcctcgac ccggacgccg
      541 ccccactccg gcaggtagat gcccggctcg accgtgatga cgtgaccggc cgccaggacg
      601 tccttcgact ctttcgaggc cgatggcccc tcgtggacgc ggacgccgac gccgtggccg
      661 aggccgtggt tgaaggcctc gccgtagccg cgcgcgtcga tgtactgacg cgctgccgcg
      721 tccatgtcgg cgccggtcgt gccgatctgg gatcgttcga tgacctcctc gacggccgct
      781 gcgacgatcg ggtagacctc acgcagcttc tcgtccggct caccgaccca gacggttcgg
      841 gtcagatcgg cgcagtagcc gtgatagcgc gcccccatgt cgatgacgat cggttcgccc
      901 tcctgaatga cccgctcgcc cggttcgtgg tgcgggcggg cggcgttcgt tccggccgcg
      961 acgatggttg ggaaggacgg cccctcggca cccagctcga cgaatgcctg atggatcttc
     1021 caggcgacct cagcttcggt ctggccggct tgaatcgtgg gcgcgacgcg gtcgaacgcc
     1081 tcgttggtga tctggaacgc cttgcggagc agcgcgatct ccgactcgga cttgacgatc
     1141 cgtagttcct cgacgaggcc gtccgcgacg acccactcgt ggtcgtgctt cttctcgttg
     1201 agctgctcgc tgataccgtg gaagacacca accagcatcg cctgggcttc gtagccgatc
     1261 ttgtggacgc cgaggtccgt caggacggcg gcgtcggcct gcggccagcc gccttcgcgc
     1321 ttgatgacgc gaacatgcgg cgcctgggct tcggcctgcg tcacgttgac gccaccagtg
     1381 acgaggacgg cgtcggtggc ggtgatgaac aggtggccgg acgactcgtt cggcgcgatg
     1441 tcgtcgccgg tgaagccggt caagtagacg cggttggagg ggtgggtgat gagcaggcaa
     1501 tcaagctgct tctcggccat cagggcgcga acgcgctcga cgcgctggct cgattcactc
     1561 accggcttgt gcctccttca gttcgaccag atccatcagg tagtggagcg ccaggacgta
     1621 cccgctggcg ccgagcccgg cgatcgtgcc gtgcgcgatg gccgagatga ccgaatggtg
     1681 ccggaactcc tcgcgggcgg cgatgttgga gaggtgcacc tcgatcgcgg gcgccggcac
     1741 ggctgcgagc gcgtcgcgca gcgcgatact gtaatgcgtc agcgcccccg gattgatgat
     1801 gataccagag gctacccagc cgtagcgctg aatctggtcg atcagcgcgc cttcgtagtt
     1861 cgattgcaat accacaagct ggaatccgcg ctcgtctgcg gcctgctgca atgcctcgtc
     1921 gatctcgtcc agcgtcgtcg tcccgtagat ctccggctgg cgccggccga gcaggttcag
     1981 gttcgggccg tggatgacca gaactgttcg accgtcaagt gcggattcgc tcatcgtatc
     2041 cagccccgtt cacgtagcat ctcgttgcca agcagaccca gcagcacttc ggagtcggtc
     2101 ccagcgtatt ccgggtggta ttcgaagcgg gcgcgctcga aatactggac gacgtacgac
     2161 tgaccgtcct ccgggttgac ctccgtgaac tcctcggtga tcggatagcc gaacgtcatc
     2221 agcccgccgt tggcttccca gtaacgctgg aaggcgccgc cgaggttatg cccggtctcc
     2281 tcgaaatacc gggcgccgga ttttgggccg gtcgcggtct cccacgggtc cataccgcgc
     2341 tgggcgagcg cccagcggcc ggtgtgaccg atcaggacgg cgaactcggt gccggcgaac
     2401 tcgggatgat actcgaagcg ggcgcgctca aaatactgaa ccgtgtagga gatgccgacc
     2461 atcgggtcgg tctcggtgaa ctcctcggtt cgggggtagc cgaagcgggc caggccgccg
     2521 ttcgccagcc agtagtcgag gaagccgtag gccagcgagt ggccggtctc gggaaagtag
     2581 atgcggtcgg acgtctgctc gaacggcggg atgcgggtag cgggcgtctg gaggctggcg
     2641 atgacggtcc aggttgccgg gtcttcgtgg ccgagccgcc agccggcgaa cccggccagc
     2701 cggtagtcgg tgacgatctt gagcttggcg tcgaagctgt cggcgttctc gaaccagact
     2761 tcgtggttgt caccggcgtc gtccttgtag cgcaggcgat tggcggcgtt ggtcttgtcg
     2821 taggtcagat cggcgtcctt acgctcggcc agcttcatcg cgctgtcgta gctgaccgac
     2881 gtggccggcg ggcctttggt cgtgttccag tcgtaaccgt agaacgggac accgaggatg
     2941 atcttctcgc gcgggatctg ggagacggcg tacttcgcga cgtcgtcaac ccaccagatc
     3001 ggcgcgacgg cgatcggatc gggccggcca gcgtagccgt agtcgtaggc catgatcgcg
     3061 atgtagtcgt tgtacttggc cagctcgctg tagtcgtagg cgccgcccca gtagttggcc
     3121 gcatcgctgg tacgggcaat gacagcctgg ctgacgatcc agccgcgtgg ctggaaggcg
     3181 gcatgcagcc gcttcatgaa gtcggtcagg atgtcatcgt cactggcgtt gatcccttcg
     3241 aagtcgatct ggatgccgtc gtagccgttg gactcaacga cggtgaccag cctggcgacg
     3301 atcgcgtcac gcttctccgg tgtctcgaac gtcttgtgga actcgtccca ctgcggctcg
     3361 gtctgaacga gcggcaggac cttgacgccg tttgcctgca acatggctgt caccgtcggc
     3421 tgggcaaacg acttggtctc gccggacggc gtgatgtgat accagtaggg cgagacgaca
     3481 tcgagatgcg acaggttggc gtcgagcgac gcccgcgacg cttcgtcgta gttgacatag
     3541 tatccccagc gcaggacggc gctggcgtca gcgacgttgt cgccgttgcg gatcgcgagt
     3601 gcactgaaga cactcagcag gaccaatccg gcgacgatcg ggatcgcggc gcggcgctgc
     3661 atcatcgact catccttgcg gtgttgcctg accggcgatc gccgcgacgt agaccgagac
     3721 tggcacgacg tcgccggttt cattgatcgt ggcttctcca ctgaagacaa tcaacggaac
     3781 cagatattcg tcaccaccgg ggctaccggc ataggagtag gcgagcgtga tgccggagac
     3841 ggtcatcgtg ccggagagtg caccactgcc accaacgctg ctcaagtcgg cttcgacgga
     3901 tgcctcgccg gcgaggacct tgttccagag cgcgtcgccg gaccagaggc cgtactcgga
     3961 gtcgacatag ccggccggcc agcggatgtt ggcctcgacg accgtcccac ctggtccgac
     4021 cgtgacggtc gccgaaggga cgaacgagag gaccggcgag acctgggccg gcttgaagag
     4081 gacgacggcg cggccggcat cgtcgtcacg gccgatgacg gcgccgccgt tcaggtcatc
     4141 gctgatgaac ccgttggcgt agatccacga ccgggccgac tcgatgacgt tcgcgtcggt
     4201 gcccaacggc agatcaggca ggctcgacgt gtagacatac tggacggtag atccgttgaa
     4261 gtagatctcg ctggttgcgc cgatcacctg gaagttgccg ggaccacctt cgactgctcc
     4321 ttcgaggccg aggttgccgg cgatcgtggc gacgtcgtcc tcggtccagg ccggccactc
     4381 aagcaggaag accggcgccg atgagggcga gtctgagagc gagacggcca gctcgtagtc
     4441 gaggtcgagc gtgcgcgaca gcccgccgct gacgatctgg gccggcggaa gcgttccgaa
     4501 gtcgccgatg gcgggcggct cctcggttgg ctccggctcc tcgatgacgg tcgtcgcggt
     4561 cggttctatc tcagccacct cctcggtcgg agttggctcg tcggtcgcga tgtcttcggt
     4621 cggtgacctc ggcgcgacct gctcggtcgt ggccgtcgga cgcgacgtcg tggccgtgtc
     4681 gggtggtgtc gtagatgttg gtggtt
//
LOCUS       DASUGJ010000295         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_184138, whole genome shotgun sequence.
ACCESSION   DASUGJ010000295 DASUGJ010000000
VERSION     DASUGJ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184138"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFV93_06545"
     CDS             complement(<1..644)
                     /locus_tag="VFV93_06545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5165038.1"
                     /translation="MTTDRTTQTRWVQENVQTWTDEHLVRWYSKWSREWAGFVAQTTP
                     PLVEQLGIAAGQRVLDLASGPGEPAITIARMVGPTGQVTATDLSAGMLECAAANAREA
                     GVTNLVTQVAPADDLPFPDGAFDRVTCRFGVMYFPDVRKALSEARRVLVPGGQAGFAV
                     WPPDDEHPYADHLALFARYFPSPEPEPGQPASDRFSDGESLGEELRAAGFVDVIV"
     gene            complement(779..1372)
                     /locus_tag="VFV93_06550"
     CDS             complement(779..1372)
                     /locus_tag="VFV93_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165039.1"
                     /translation="MRPFSQRRLIFVIVMVVAFLMPVGVAGAAGFTPPIPSDATDAHC
                     SSSPQGTICHYTRVRAGSNLPNWNDVVCDGFTISYTFTATGGYRDVYDQAGNLMKEVV
                     QIGFTGTLMNASDPSKTVPYAGHWTHTIDMRAGTDTITGIHQHVVLPGQGLVSANIGR
                     IVFTLDPTVLTPEFVAGQWDAFPGVPMDTEALCHALA"
     gene            complement(1407..>2606)
                     /locus_tag="VFV93_06555"
     CDS             complement(1407..>2606)
                     /locus_tag="VFV93_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165040.1"
                     /translation="RVDPATNTLATTFEVAQASELVSVAAGAGSIWVGDPVKATLYRL
                     DATTGDLLAATELGPPLIGITFTEGSLWAALEDSGVVARVNLPTAQPSPTSTLPEFGT
                     IAATVEVGESPRQFAAGAGALWVTNADDGTVSRIDLTTNEVVASIVVVPPEEFVTGVN
                     GATQVDAIAVDDSAVWVSAINSSQLMQIDPTTNQVVERYRLPAGVHGLTAGGGALWGR
                     SWTSNQILRIDPATGSVVASVPVEMPASAVYLADALWVQRPGEVVKIDPATNTIVARV
                     TYADTAGDPTAPRRMMVAAAGRLWVSSDSWHEVLEIDPATATVAGTVPLPFAIDTIVV
                     AGSDANLWACPCFGNNNEQVWQLDLAEDEWNAVQTVPFVLGIDFDGGTLWGLGSHGEV
                     IRIDLLP"
BASE COUNT          454 a          831 c          853 g          468 t
ORIGIN      
        1 acaatgacgt cgacgaaccc ggcggcgcgt aactcctcgc cgagcgactc cccatcgctg
       61 aagcgatcgg atgctggctg tcctggttct ggctcgggcg aggggaaata cctcgcgaag
      121 agggcgagat ggtcggcgta ggggtgttcg tcgtccggtg gccaaacggc gaacccggcc
      181 tgtccaccgg ggaccagcac tcgccgcgct tcggacagcg cctttcggac atcagggaag
      241 tacatcactc caaaccggca ggtgacccga tcgaaggcgc cgtccgggaa tggcagatca
      301 tctgccggcg cgacctgggt gacgagattc gtgaccccgg cctcccgcgc attggcggcc
      361 gcgcactcga gcatgccggc cgagagatcg gtcgcggtca cctgaccggt cgggccgacc
      421 atgcgggcga tcgtgatggc cggctcgccg gggccgctgg cgaggtcgag cacccgttgc
      481 cctgcggcaa tcccaagttg ctcaacgagg ggtggcgtcg tctgagcaac gaatccggcc
      541 cactcgcgac tccacttcga ataccagcgg acgaggtgct cgtccgtcca cgtttggaca
      601 ttctcctgta cccagcgtgt ttgcgtcgtg cggtcggttg tcatggcgtc gtgctccttt
      661 gcgtcagatc atcactgata cacgtcactg cacgtgggtc tacgatgtga caacgttgcg
      721 cctgccggta tgaaaccgga cggggtcgag accccgtccg gtctggctcg ccatgtggct
      781 atgccagcgc gtgacacagc gcctcggtgt ccatcggcac acccggaaag gcgtcccatt
      841 gaccggcgac gaactcgggt gtgagcaccg tcgggtcgag cgtgaagacg atgcgaccga
      901 tgttggcaga gacgagccct tgccccggta gcactacgtg ctggtgaatg ccggtgatgg
      961 tgtcggttcc agcacgcatg tctatcgtgt gggtccagtg accggcgtat ggcaccgtct
     1021 tcgacgggtc acttgcgttc atcagtgttc cggtgaaccc gatctggaca acttccttca
     1081 tgaggttgcc cgcctgatcg tagacgtccc ggtaccctcc ggttgccgtg aacgtgtacg
     1141 agatggtgaa gccgtcacag acaacgtcgt tccagttcgg cagattggag ccggcgcgga
     1201 cgcgtgtata gtggcagatg gttccctgcg gactactact gcagtgcgca tcggtcgcgt
     1261 ctgacggtat cggaggagtg aagccggcgg ccccggcaac cccgaccggc atgaggaatg
     1321 caacaaccat cacgatgacg aagatgaggc ggcgctgaga gaacggtcgc atctgacact
     1381 cctttcacgt tgctggttca gtgtggctac ggcaacaggt cgatccggat gacttcaccg
     1441 tggctgccga gcccccagag ggtgccgccg tcgaagtcga ttccgagtac gaacggcacc
     1501 gtctggacgg cgttccactc gtcttcggcc agatcgagct gccagacctg ctcgttgttg
     1561 ttgccgaagc atgggcaggc ccacagattc gcgtcgctcc cggcgacgac gatcgtgtca
     1621 atggcgaacg gcagtggcac tgtcccggcg accgtggcag ttgctggatc gatctcgagt
     1681 acctcgtgcc agctgtcaga cgacacccac aggcgtccag cggcagcgac catcatgcgt
     1741 cgcggcgctg ttgggtcgcc cgccgtgtcc gcgtaggtga cgcgcgcgac gatggtattc
     1801 gtggccgggt cgatcttgac gacctcgcct gggcgctgca cccagagtgc gtcggccaga
     1861 tagaccgccg aggccggcat ctcgaccggg accgaggcga cgacgctgcc cgtcgccgga
     1921 tcgatccgca ggatctggtt gctggtccag gatcgacccc agagcgcacc accgccggcg
     1981 gtcagcccat ggacgccggc cggcagccgg tagcgctcga ccacctgatt cgttgttggg
     2041 tcgatttgca tgagttgaga actgttgatc gctgagaccc agacggcact gtcgtcaacc
     2101 gcaatcgcgt caacctgggt cgccccattg acaccggtga cgaactcttc cggcggaacg
     2161 accacgatcg aggcgacgac ctcattcgtc gtgaggtcga tgcggctgac cgtgccgtca
     2221 tcggcgttcg tcacccagag cgctcccgca ccagcggcga actgacgcgg gctctctccg
     2281 acctcgaccg tcgcggcgat cgtgccgaac tccggcagcg tgctggtcgg gctcggctgg
     2341 gccgttggaa gattgacgcg cgccacaacg ccggagtctt ccagcgccgc ccagagtgat
     2401 ccctccgtga aggtgatgcc gatgagcggc ggcccaagct ccgtcgcagc gaggagatcg
     2461 cccgtcgtag catcgagacg atacaacgtt gccttgacag gatcgcctac ccagatcgag
     2521 ccggcgccag ccgcgacaga gacgagttcc gaggcctgag ccacctcgaa cgtcgtcgcg
     2581 agtgtattag tcgccggatc gacgcg
//
LOCUS       DASUGJ010000296         2970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1712594, whole genome shotgun sequence.
ACCESSION   DASUGJ010000296 DASUGJ010000000
VERSION     DASUGJ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712594"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /gene="larB"
                     /locus_tag="VFV93_06560"
     CDS             complement(<1..639)
                     /gene="larB"
                     /locus_tag="VFV93_06560"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX5165041.1"
                     /translation="MRDPFSAVEAALSGGLDDTGDESVRIDFDRQQRTGIPEVIYGDS
                     KSDAQVLMSCTKMLERRPRTIVSRIAIERAESIASSIGLDIRVEQPFAGQTVVLSRGD
                     ARPPMPSGFVGIITAGTSDLPAAGEAATVISEIGCRVELVADVGVAGLHRLVQPLRRI
                     INAGADVIIVAAGMDGALPSVVSGLVDIPVIGLPTSVGYGVAAGGQAALMTML"
     gene            complement(632..1432)
                     /locus_tag="VFV93_06565"
     CDS             complement(632..1432)
                     /locus_tag="VFV93_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX5165042.1"
                     /translation="MPSRKVRADELVVQQGLAESRSKAKALIVAGDVRSGDRLIQKPA
                     EMLDVDLPLELRAKLPFVSRGGLKLDHALTEFEIDVTGAVAADLGASTGGFTDVLLQR
                     GARRVYAIDVGYGQLDYRLRSDERVVVMERVNARYLEALPEPAGFVCVDVSFISLEHI
                     LPVAERLLEPDGNLVTLIKPQFEAGREAVGRGGVVRDDRTRKAVVEHVVDLAAQQRFA
                     LQGLTTSPITGPAGNVEYLAWFRRDAVASSGAVLIERLFRRKETNQHA"
     gene            complement(1435..1932)
                     /gene="ruvC"
                     /locus_tag="VFV93_06570"
     CDS             complement(1435..1932)
                     /gene="ruvC"
                     /locus_tag="VFV93_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX5165043.1"
                     /translation="MMTLGVDPGTALLGFGLVRGDDDPELVTFGVVETTKSEPMPERL
                     KRLYDAVWELIREHEPDVLAIEQLFFSRNVTTALAVGQARGVVLLAAAQHGMEVYEYK
                     PSEVKQTIAGHGNADKPQMQEMVRMLLGLPQIPKPDDAADALAVALCHVQMSRLSRRF
                     DIASR"
     gene            2197..2343
                     /locus_tag="VFV93_06575"
                     /pseudo
     CDS             2197..2343
                     /locus_tag="VFV93_06575"
                     /inference="COORDINATES: protein motif:HMM:NF015912.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            2467..2970
                     /locus_tag="VFV93_06580"
                     /pseudo
     CDS             2467..2970
                     /locus_tag="VFV93_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          583 a          983 c          898 g          506 t
ORIGIN      
        1 cagcatcgtc atcaacgccg cctgcccacc ggccgccacg ccatagccga cggacgttgg
       61 taggccgatg accggaatgt ccaccagccc tgagacgacc gacggcaacg ccccatccat
      121 cccggccgcg acgatgatga catccgcgcc ggcgttgatg atccgtcgta gcggctggac
      181 gagccggtgc agaccggcaa cgccgacatc ggcaacgagc tccacccggc agccgatctc
      241 gctgataacg gtggctgcct cgccggccgc cggcaggtcg gacgttccgg ccgtgatgat
      301 gccgacgaac ccagacggca tcggcggacg agcatcgcca cggctgagga cgaccgtttg
      361 tcccgcgaag ggttgctcga cacgtatgtc gaggccgatc gagctggcga tcgattcggc
      421 ccgctcgatc gcgatccgcg agacgatggt gcgcggccgg cgctccagca tcttcgtaca
      481 cgacatgagc acctgtgcat ccgacttgct gtcgccatag atcacttccg ggatgccggt
      541 tcgctgctgc cggtcgaagt cgatccggac actctcgtcg ccggtgtcat cgagacctcc
      601 cgacagcgca gcttcaaccg ccgagaacgg gtcacgcatg ctggttcgtt tcctttcgcc
      661 tgaagaggcg ttcaatcaag actgcaccac tcgatgcgac ggcatcccgg cgaaaccagg
      721 ccaggtattc gacgttgccg gccggaccgg tgatcggcga tgtggtcaga ccctggagcg
      781 caaaccgttg ctgagcggcc aggtccacga cgtgctcgac gaccgccttg cgggtacgat
      841 cgtcgcgaac gacacctccg cgcccaaccg cttcacgccc ggcctcgaac tgcggcttga
      901 tcaacgtcac gagattgccg tcgggctcca gcaggcgttc agcaaccggc aagatgtgct
      961 ccagcgagat gaacgagacg tcaacacaga cgaaccccgc cggctccggc aatgcttcca
     1021 gatagcgggc gttgacccgc tccatgacga cgacacgctc gtcggaacgc agccggtagt
     1081 cgagctgtcc gtagccgaca tcgatggcgt agacccgtct ggcgccacgc tggagcagaa
     1141 cgtcggtaaa tccgccggtc gacgcaccca gatcggccgc aaccgcgccg gtcacatcga
     1201 tctcgaactc ggtcagcgcg tggtcgagct tgaggccacc acggctaacg aacggcagtt
     1261 tggcgcgcag ttccaagggc aagtccacat ccaacatctc ggccggcttc tggatcaggc
     1321 ggtcgcccga acgaacatca ccggcgacga tcagcgcctt cgccttcgat cgagattcag
     1381 ccaggccctg ctggacgacc agctcatctg cgcgcacctt gcgggacggc atcgtcagcg
     1441 gctcgcgatg tcgaagcgac gcgagagccg gctcatctgg acatgacaga gggcgacggc
     1501 cagcgcgtcg gcggcgtcgt cgggctttgg aatttgcggc agcccgagca gcatccgcac
     1561 catctcctgc atctgcggct tgtcggcgtt gccgtgtcca gcaatcgtct gcttgacctc
     1621 agacggtttg tattcgtaga cctccatccc gtgctgagcc gctgccagca ggacgacgcc
     1681 gcgcgcctga ccaacggcca gcgccgtcgt cacgttccgc gagaagaaga gctgctcgat
     1741 cgccagcaca tccggttcgt gctcgcgaat caactcccag acggcgtcgt agaggcgctt
     1801 caatcgctcc ggcatcggct cggatttggt cgtctctaca acgccaaatg tcaccagttc
     1861 ggggtcatcg tcaccacgta caagcccaaa gccgagcagc gccgtccccg ggtcaacacc
     1921 aagcgtcatc atgccgaaac tgccccaatc tgccggttcg gcaagttcgc agaccggtct
     1981 cgtacgaaag gctgttgaaa tatgggtaaa cctgccgtat actgctcgaa acacatgtta
     2041 cagacgcccc ccgtaacgga tcgacgagaa agtcaacgct cgcacactca accggcgtag
     2101 ccgtgatcct gtcgtgcccg ccggcctcct cagttgagcg ctaccctcgc accgttgcag
     2161 cgtctgtaga ctactgggcg ggagggaaac agcagcgtgg ttgcgagcct gattgccaag
     2221 ggtaaggatc aggggttcct actttctgag gaagttctcg aagcgtttcc gcgaatagaa
     2281 gaggacatcg ccgccgtcga cgagttctgg tcgtcacttc tggagaacgg agtcgatgtc
     2341 atcgaccagc cgccagtgcc gacgaccctc aacagtcgtc atcagtttac tcgccaccgg
     2401 gcggttgcgc cgacaaccga cgtcattccc gatccattcg caaaaccaga gggccgtgac
     2461 gagtcgaccg acacgctctc gcagggtgtc gccgattcgg tccgcctcta tctacaggag
     2521 atcggcgaga ccgatctgct gacgatgcac gaagaagtct ggctggcgaa gaggatggta
     2581 cgcggcctgg cagctagcca gcggttggct gccggtgagt tcgcaagcga ggacgagcgc
     2641 cgcgagctca tcgaggaccg cgaagatggg gaacgggcac gcgcccacct cattcaggcg
     2701 aacctgaggc tggtcgtctc ggtcgccaag aagtacgtcg ggcgtggcct gtcgttcctg
     2761 gacctgatcc aggagggcaa catcggcctg atgaaggcca ccgacaagtt cgactacaac
     2821 cgcggcttca agttctcaac ttacgcgacg tggtggatcc ggcaggcgat cacgcgtgcg
     2881 atcgccgacc aggcgcgcac gatccgcatc ccagttcaca tggtcgagac gatcaaccgc
     2941 ctgattcgcg tgtcgcgtgg cctgctccag
//
LOCUS       DASUGJ010000297         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1433215, whole genome shotgun sequence.
ACCESSION   DASUGJ010000297 DASUGJ010000000
VERSION     DASUGJ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433215"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFV93_06585"
     CDS             <1..350
                     /locus_tag="VFV93_06585"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX5165044.1"
                     /translation="IGLNLLLVPQFGVAAAAVNTTIGYGALFVGVSWYMRRVCDPAIS
                     YDGASILRAILITGLPAIAAAILLPSDTPVGLLARVGAMLFVAILLGAGPLRPESGSL
                     MRLVRHVPTGGPA"
     gene            347..1327
                     /locus_tag="VFV93_06590"
     CDS             347..1327
                     /locus_tag="VFV93_06590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX5165045.1"
                     /translation="MSDSTVSLVIRCFNEEAHLGRLLRGVARQTRRPDQIVIVDSGST
                     DSTLEIAAAFDTEIHHVDPARFSFGRSLNIGCRASKGDLIVIASAHVYPLYDSWLAEL
                     TAPFDDREVVLSYGRQEGGSDTRYSEKRVMARWFPPTSAARQDHPFCNNANAAVRRSA
                     WEQQPYDEELTGLEDLDWAKRQMDAGRAIAYVATAPVVHAHDETWSQLVNRYRREAIG
                     HKRIFHERGMSGFEAMRLAAGNVASDYVHATREGELLSNIGAIPSFRIAQFWGTYLGF
                     RHRGDASAALRRRFYYPHGWRRQPTSEQPHGAQRIDYEDRDHRGVDVRAD"
     gene            1311..1946
                     /locus_tag="VFV93_06595"
     CDS             1311..1946
                     /locus_tag="VFV93_06595"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5165046.1"
                     /translation="MSEPIDISWPLRPGMPAWPNSVGYQRRLVAAIADGAQANVSTIQ
                     MDVHCGTHVEGPLHFIEDGASVDAFGLGPFIGPSRVVYIPDADEIGPSHLEECIGADH
                     VDRLLIRTRNSDRVAGGGEFATDFAALGLEGATWLANRGVLLVGIDYLSIQLFGGDPE
                     THRVLMRAGVAILEGLKLHDVAPGDYRLTCLPLALQDAEAAPARAVLEPME"
     gene            1943..>2435
                     /locus_tag="VFV93_06600"
     CDS             1943..>2435
                     /locus_tag="VFV93_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165047.1"
                     /translation="MTYAVAALVPMRHESERVPGKNYRLLAGKPLFHYILQSLLASPR
                     VSDVVIDTDSELIRDDVAVAFPSVRVIERPPHLRDGKISMNAVLLNDVEQIGADIYLQ
                     THSTNPFLKSGTVTAAVDAFLGNPGCDSLFSVTRRQTRLWSADGRPLNHDPEQLLRTQ
                     DLDP"
BASE COUNT          449 a          786 c          744 g          456 t
ORIGIN      
        1 atattggcct aaaccttctc cttgtcccac agttcggcgt cgccgcagct gcggtcaaca
       61 cgacgattgg ctacggagca ctgttcgtcg gagtcagttg gtacatgcgg cgggtgtgcg
      121 accctgcgat cagctatgac ggcgcgagca tcctcagagc catcctcatc acagggctgc
      181 cggccatcgc ggccgcaatc ctgttgccgt ccgacacccc cgtgggtctc ttggctcgag
      241 tcggcgcgat gctgttcgtt gcgatcctgc ttggcgccgg gcccctgcgg ccagaaagtg
      301 gctcattgat gagattggtg cgtcacgtcc caaccggggg accagcatga gcgactcgac
      361 ggtctcgctg gtcattcgct gcttcaacga ggaagcacac ctcggtcgcc tgctgagggg
      421 cgttgcccgg cagacccgca gacctgatca gatcgtgatc gtggattctg gatcgaccga
      481 ctccaccctc gagatcgcag ctgcattcga taccgagatc caccacgttg acccggcgcg
      541 cttctcgttt ggtcgatcgt tgaacatcgg ttgtcgggca tcgaagggcg acctgatcgt
      601 gatagccagc gcacacgttt acccgctcta tgacagctgg ctggccgagc tcaccgcgcc
      661 gttcgacgac cgtgaggtcg tcctgtccta cggccgccag gaaggcggca gtgacacgag
      721 atactccgag aagcgagtga tggcccgttg gtttccaccc acgtccgccg ctcggcaaga
      781 tcacccgttc tgcaataacg cgaacgcggc agtccggcga tcggcgtggg agcagcaacc
      841 gtatgacgag gaactgacgg gcctcgagga cctcgattgg gcgaagcggc agatggacgc
      901 agggcgggca atcgcgtatg tcgccaccgc tccagttgta cacgctcacg acgagacctg
      961 gtcgcagctc gtgaaccgct atcgccgcga agcgatcgga cacaagcgca tctttcacga
     1021 gcgtggcatg agcggcttcg aggcaatgcg gctcgctgct ggaaacgtag cgagcgatta
     1081 cgtccatgcc acccgagaag gcgagctgct ctcgaatatc ggtgccattc cgtcgttccg
     1141 catcgcccag ttctggggca cctatctcgg cttccgacac cgcggtgatg catccgccgc
     1201 gttaaggagg cgcttctact atccacatgg gtggcgccgc caaccaacct ccgaacagcc
     1261 gcacggtgcg caacgcatcg actatgagga tcgagaccat cgaggggtcg atgtccgagc
     1321 cgattgacat ctcctggccg ctccgaccag ggatgccggc ttggcctaac agcgtcggct
     1381 accagcgtcg actggtggcg gcgatagcgg atggggccca ggccaacgtc agcacgatcc
     1441 agatggacgt tcactgcgga acgcacgtcg aagggccact ccatttcata gaggacggcg
     1501 catccgtgga cgccttcggc ctgggaccat tcattgggcc gtcacgagtc gtgtacatcc
     1561 ccgacgccga cgagattggt ccatcccatc tcgaggagtg catcggagcc gatcacgtcg
     1621 accgtctact gattcgaact cggaactcgg acagagtcgc cggcggcggt gagtttgcta
     1681 ccgacttcgc tgcgctcggg ctggaaggcg ccacgtggtt ggccaaccgg ggagtcctgc
     1741 tcgtcggtat cgactacctg tcgatccagc tattcggtgg cgacccggag acccatcggg
     1801 tgctcatgcg ggccggggtc gcaatcctgg agggattgaa actgcacgat gttgcacccg
     1861 gtgactaccg cctcacctgt ttgccactag cgctccagga cgctgaggcg gcgcccgcac
     1921 gagcggtcct ggagccaatg gagtgaccta cgccgtcgcg gcgctcgtgc cgatgcggca
     1981 tgagagcgag cgcgttccgg gcaagaacta tcggctgctc gccggcaagc cactcttcca
     2041 ctacatcctc caatcgttgc tggcgagccc tcgcgtatcc gacgtcgtga ttgacaccga
     2101 cagcgagctc attcgcgatg acgtggctgt cgcattccct tcggtgcgcg tgatcgaacg
     2161 gccgccacac ctgcgggacg ggaagatctc aatgaatgcc gtcctgctca acgatgtcga
     2221 gcagatcggg gccgacatct accttcagac gcacagcacg aatccatttc tgaaatccgg
     2281 gaccgtgacc gccgccgttg atgcattcct cggcaacccc ggctgtgatt cgcttttcag
     2341 cgtcacgcgt cgccagaccc ggctctggag cgccgacggt cggccgctaa accatgatcc
     2401 agaacagctg ctccgaactc aggatctcga ccccg
//
LOCUS       DASUGJ010000298         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_739923, whole genome shotgun sequence.
ACCESSION   DASUGJ010000298 DASUGJ010000000
VERSION     DASUGJ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739923"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFV93_06605"
     CDS             complement(<1..575)
                     /locus_tag="VFV93_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5165048.1"
                     /translation="MAVEKSVVAQQLAAIVGEAHVVADQAGLSVYQYDASDEAIAGIH
                     RPLLAVLPATAEEVRDVVRVALANDLPVVARGAGTGLAGGAIACRGGIMIVMTRMTRI
                     LEVNEIDRYAIVEPGLINLDLSLAIADRGYFFAPDPASQRACTIGGNIANNSGGPHCL
                     KYGVTSNHILGLEVVLPSGELTWVGGASAETA"
     gene            658..1146
                     /locus_tag="VFV93_06610"
     CDS             658..1146
                     /locus_tag="VFV93_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX5165049.1"
                     /translation="MPTESSQDTHTNWIIVGSPDNFAATRSLDFTIQGIKSRHRKKAE
                     TMRPGDRIVWYITGQKAFAGYATITSDYYEDHTPIWKSRDPKKDAEDYPFRVKIQAEV
                     VLDDDAFIPAEPIARQMTYVAKWPAANWTLAFQGNVHKIDDADFDLIAGALREATAVA
                     AG"
     gene            1176..2153
                     /locus_tag="VFV93_06615"
     CDS             1176..2153
                     /locus_tag="VFV93_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5165050.1"
                     /translation="MADRPTLDDIHAAADRLRGVAVRTPLVPLHAYDGKSDIFLKLEN
                     LQPIGSFKLRGIFNAVAALTPERRASGISTTSSGNTAQALAWAGRHFGVTARSLMPEN
                     APVSKIEAMRSYGGTPVLVTREHLFSYMADHGWDEEPYAFIHPWIDPVVAAGNGTIGL
                     EILEDVPDVETIFVPVGGGGLSSGIGTAIKAVKPEVRIVAVEPEGCCALSQSLEAGQP
                     MSVACNTICDGVAVPLVVPEVFEILRDVIDDVVLVSDDDVKKTIKRLALRNKLVTEGA
                     GALATTAALQTPSASRGRSVCIVSGGSIDPDFLAAILTDPSLEEDKRRS"
BASE COUNT          434 a          806 c          644 g          349 t
ORIGIN      
        1 gccgtctcgg ccgatgcccc gccaacccag gtaagctccc cgctcggcag gacaacttcg
       61 agaccgagaa tatggttgct ggtgacgccg tatttcaggc aatgcggccc gcccgagttg
      121 ttggcgatgt tgccaccgat cgtgcaggcc cgctggctgg caggatccgg tgcgaagaag
      181 tagccccggt cggcaatcgc cagcgacaga tcgaggttga tcaggcccgg ctcgacgatc
      241 gcgtaacggt cgatctcgtt gacctcgaga atccgcgtca tccgcgtcat cacgatcatg
      301 atcccgccgc ggcaggcaat cgccccacca gccagtcccg tccccgcccc tcgcgcgacg
      361 accggcagat cgttcgccag cgccacccgg acgacatcgc gaacctcctc ggccgtcgcc
      421 ggcaggacag ccagcagcgg ccggtggatt ccggcaatcg cttcgtcgga cgcgtcatac
      481 tgatagaccg acaggccggc ctggtcggcg acgacatgcg cctcgccaac gatcgccgcc
      541 agttgctgcg caacgacgct cttctcgact gccatctctc gcctcaccca ctagaccgaa
      601 cgtacacgcg ctggcatact ggccgcgacc gccctgacca acgacgggag taacgcgatg
      661 cccaccgaaa gcagccagga cacgcacacg aactggatca tcgtcggttc gccagataac
      721 ttcgccgcta cccgcagtct cgacttcacg atccagggga tcaagagccg ccaccgcaag
      781 aaggccgaaa cgatgcgacc cggcgaccgg atcgtctggt acattaccgg ccagaaggcc
      841 tttgccggct acgcgacgat caccagtgac tactatgaag atcatacccc gatctggaag
      901 agccgcgacc cgaaaaagga tgctgaggac tatccatttc gcgtcaagat ccaggccgag
      961 gtcgttctcg acgacgacgc gttcatcccg gccgagccga tcgcccgcca gatgacctac
     1021 gtggcgaagt ggccggccgc caactggacg cttgcgttcc agggcaacgt ccacaagatc
     1081 gacgacgccg acttcgacct gatcgccggc gcgctgcggg aagcaacggc cgtcgcggcc
     1141 ggctagcaac ggcgggcacg accaaggggg attgagtggc agatcgaccg acgcttgacg
     1201 atattcacgc cgccgccgac cgcctgcgcg gcgtcgccgt gcggacgccg ctcgtcccgc
     1261 tccacgccta cgacggcaag agcgacatct tcctcaagct cgaaaacctc cagccgatcg
     1321 gttcgttcaa gctacgcggc atcttcaacg ccgtcgccgc cctgacaccc gagcgccgag
     1381 catccggcat cagcacgacc agttccggca acaccgccca ggcactggcc tgggcaggcc
     1441 gtcacttcgg cgtcaccgcc cgcagcctga tgcctgagaa cgcgcccgtc tccaagatcg
     1501 aagcaatgcg ctcgtacggc gggacgccgg tcctcgtcac ccgcgagcat ctctttagct
     1561 atatggccga tcacggctgg gacgaagagc cgtatgcctt catccacccg tggatcgacc
     1621 cagtcgtggc agccggcaac ggcacgatcg gactcgagat cctcgaagac gttcccgacg
     1681 ttgagacgat cttcgttccc gtcggcggcg gcggtctcag cagcggcatc ggcactgcca
     1741 tcaaggccgt caagccagag gttcgcatcg tcgctgtcga acccgagggc tgctgcgccc
     1801 tctctcagag cctcgaagcc ggccagccga tgtccgtcgc ctgcaacacc atctgcgacg
     1861 gcgtcgccgt cccgttggtc gtcccggagg tcttcgagat cctgcgcgac gtgatcgacg
     1921 atgtcgtcct cgtttcggac gacgacgtca agaagacgat caagcgcctc gccctgcgca
     1981 acaagctcgt caccgaaggc gccggcgcgc tggcaaccac tgccgccctc cagaccccat
     2041 ccgcctcacg cggcagatcc gtctgcatcg tctccggcgg cagcatcgat cccgacttcc
     2101 tcgccgcgat cctgaccgat ccgtcgctcg aggaagacaa acgccgctcc taatccgcaa
     2161 tagaacaatc cggtaggggc gatatcgcaa tatcgcccac tgtttccacc gcaacgcgtc
     2221 gggcagggaa ctg
//
LOCUS       DASUGJ010000299         3556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1850855, whole genome shotgun sequence.
ACCESSION   DASUGJ010000299 DASUGJ010000000
VERSION     DASUGJ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850855"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFV93_06620"
     CDS             <1..498
                     /locus_tag="VFV93_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165051.1"
                     /translation="GLHLTIATPPGYEPDETLFAKSWGIAKKMGGSVNIMHEPADAVK
                     GADAVYTDAWLSMGDSELTRAERFAALTPYRVTPQMMAQANPDAVFMHCLPAHRGEEV
                     TAEVIDGPQSIVFDQAENRLHTGQAVLYALITGKLTGTSTTIPVTTTANGKVKSSIPS
                     AVHAD"
     gene            724..1002
                     /locus_tag="VFV93_06625"
                     /pseudo
     CDS             724..1002
                     /locus_tag="VFV93_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(1372..>3556)
                     /locus_tag="VFV93_06630"
     CDS             complement(1372..>3556)
                     /locus_tag="VFV93_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX5165052.1"
                     /translation="AGAKIAVMDPRLSNTASMANYWMAPAPGTDTIVLLALANQLIQH
                     DWFDSEFVRRWTNWDEFMAVMHPGVEPTFANFVAILKETYAPYTPEMAEAASGVAAGT
                     IVEVAREIGRAGSAFSAHTWRAASAGHLGGWTVPRALFFLNVLTGSVGTEGGTMPNVW
                     NKWVPRPFAEPAKQKVWNELTWPKEYPLTHHEMSILLPHFLKEGRGKLAVYFTRVYNP
                     LWTNPDGFSWMESLKDENQIGLHAALTPTWSESAWFADYVLPMGHGPERHDSLSYETH
                     NAQWVGFRQPVVRVAMERMGKPVALTRDANPGEVWEENEFWIELSWKIDPDGSLGIRK
                     LYESPYRPGEKLTVDEYYQWMFENSVPGLPEAAAAEGLTPLEYMRRYSAFEIRKAPER
                     QFNDVVPAAELEGAVEQPNGVIVSPSPRPHAPNVVPMPGMPPGSMDGRAVGVVVDGVP
                     QRGFPTPSGKLEFFSRTLYEWGWPELAIPCHVESQVAPNEIDASRGEMVLLPNYRIPT
                     LVHTRTNNSKWLNEISHSNPLWLHPSDAARIGVATGDLVRIETEIGHFVDKVWVTEGM
                     RPGVAACSHHLGRWRLQDGEGNERWSSALVDIQQTEPGVYKMQQIHGVGPFESDDPDS
                     SKIWWQDAGVHQNLTFPVQPDPISGAHAWHQKVRVVKAGADDRYGDIVVDTTKSHEAY
                     KRWLAMTRPAPGPGNLRRPLWLLRPYKPDPSAFAVDGPVGRRERN"
BASE COUNT          610 a         1157 c         1178 g          611 t
ORIGIN      
        1 ggcctgcacc tgacgatcgc gacgcctccc ggctacgagc cggacgagac gctgttcgcc
       61 aagtcgtggg gcattgccaa gaagatgggc ggctcggtca acatcatgca cgagccggcc
      121 gacgccgtga agggcgccga cgccgtctac accgacgcct ggctctcgat gggtgactcc
      181 gagctgactc gcgccgagcg cttcgccgcc ctgacgccct accgggtgac gccgcagatg
      241 atggcccagg ccaaccccga cgccgtcttc atgcactgcc tgccggccca ccggggcgag
      301 gaggtcaccg ccgaggtcat cgacggcccg cagtcgatcg tcttcgacca ggccgagaac
      361 cggctccaca ccggccaggc cgtcctctac gccctcatca ccggcaagct gaccggcacc
      421 agcacgacga tcccggtcac gacgaccgcc aacggcaagg tgaagtcgtc aatcccgtcg
      481 gccgttcacg ccgactgacc gacgccaata ccactccacc ctgttctggc caatggaggg
      541 tgggaacaca aagacggctc tccggagccg tctttgtgct cgttggggga agtccgttct
      601 acaatgggct aaccagagtt cgactgccga cgaggagacg atcccttgcc ctgtcatcct
      661 gagccgcagc gaaggatcgc ttaacctcaa ccacaggagc gcgccacgaa gaagtactac
      721 gcctacatca tggccagcac agctggtcgg accgccacgc tctacaccgg cgtgaccaat
      781 gatctcgaac gccgtgcctc ggaacatcgg cagcgcaaca aggacacctt cgcgggcaag
      841 tacggcaccg tccgcgtcgt ctggtatcag gagttcaatc aggtcctcga cgcgatcgat
      901 gccgagaagc gcatcaaaag ctggcggcgt tcgaagaaga tcgagttgat cgagtcggag
      961 aatcccgagt ggcgggatct ggtggctggt gaagaacgtt agttgggtgc tgccgatcgg
     1021 cgcgctcctg ccacctgcgc taagcgatcc ttcgctgcgg ctcaggatga cagggcaagg
     1081 ggttcggccc tcaactacat tgcgactctg gtgacctgtg aaacgtgtgg atgaagcgag
     1141 catggacgac caagacatcg acacgatgat gcgtccgaag gaaccaatcc caaagttcat
     1201 cctatgaaga ggaagctgaa ttctaggata cccatgacgt gaccgcctat tgggacgact
     1261 tcgcgccaac gcagatgtcc tccaagggag accttatgga gggtttgttc gttcggctcg
     1321 acgctgggac gttgacgcgt atccgagagg cagcagatga ctgcgaacgg ctcaattccg
     1381 ctcccgcctc ccaaccggtc catctaccgc gaaggcgctc ggatccggct tgtaggggcg
     1441 cagcagccac agcgggcgtc gcaggttgcc ggggccgggg gctgggcggg tcatcgccag
     1501 ccagcgtttg taggcttcgt gggacttggt tgtgtctacg acaatgtcgc cgtagcggtc
     1561 gtcggcgccg gctttgacga cgcggacttt ctggtgccag gcgtgggcgc cggagatcgg
     1621 atcgggctgg accgggaagg tcaggttctg gtggacgccg gcgtcctgcc accagatctt
     1681 gctggagtcg gggtcatcgc tctcgaacgg gccgacgccg tggatctgct gcatcttgta
     1741 gacgcccggc tcggtctgct ggatatcgac cagcgccgag gaccagcgct cgttgccctc
     1801 gccatcctgc aaccgccagc gtccgaggtg atgcgagcag gcagccacgc cggggcgcat
     1861 cccttccgtc acccagacct tgtcgacgaa gtggccgatc tcggtctcga tccggaccag
     1921 gtcgccggtt gcgacgccga tccgggccgc gtcggacgga tgcagccaga gcgggttcga
     1981 gtggctgatc tcgttgagcc acttgctgtt gttggtccgc gtgtggacca atgtcgggat
     2041 gcggtagttc ggcagtagga ccatctcgcc cctgctggcg tcgatctcgt tcggcgcgac
     2101 ctggctctcg acgtggcagg ggatcgccag ttccggccag ccccactcgt agagggtccg
     2161 tgagaagaac tccagcttac cggaaggcgt tgggaagccc cgctgcggga cgccatcgac
     2221 aacgacaccg acggcgcggc catccatcga gccgggtggc atgccgggca tcgggacgac
     2281 gttcggggcg tgcgggcggg gcgacgggct gacgatgacg ccgttcggct gctcgactgc
     2341 cccctccagc tcggcggccg ggacgacgtc gttgaactgg cgctcgggcg ccttgcggat
     2401 ctcgaaggcg ctgtagcggc gcatgtactc cagcggcgtc agcccctcgg cggcggccgc
     2461 ttccggaaga ccgggcaccg agttctcgaa catccactgg taatactcat cgaccgtcag
     2521 cttctccccg gggcgatagg gcgattcgta gagcttgcgg atgccgagcg agccgtccgg
     2581 gtcgatcttc caggagagct cgatccagaa ctcgttctcc tcccagacct cgccggggtt
     2641 ggcgtcgcgg gtcagcgcca ccggcttgcc catccgctcc atggcgacgc ggacgaccgg
     2701 ctggcggaag ccgacccact gggcgttgtg cgtctcgtac gagaggctgt cgtgccgctc
     2761 cggtccgtga cccatcggca ggacgtagtc ggcgaaccag gcgctctccg accaggtcgg
     2821 cgtcagggcc gcgtgcaggc cgatctgatt ttcgtccttg agcgactcca tccacgagaa
     2881 gccgtccggg ttggtccaga gcgggttgta gacgcgggtg aagtagacgg ccagcttgcc
     2941 gcgcccctcc ttgaggaagt gcggcagcag aatgctcatc tcgtggtggg tcagcgggta
     3001 ctccttcggc caggtcagct cgttccagac cttctgcttg gccggctcgg cgaacggccg
     3061 cggcacccac ttgttccaga cgttcggcat cgtcccgccc tcggtgccga ccgagccggt
     3121 cagcacgttg aggaagaaga gcgcgcgggg taccgtccag ccgccgaggt gtcccgccga
     3181 ggctgcccgc caggtgtggg ccgagaaagc cgagcctgcc cggccgatct cccgcgcgac
     3241 ctcgacgatc gtcccggccg cgacgccgct ggccgcctcg gccatctctg gcgtgtaggg
     3301 ggcgtaggtc tccttgagga tcgcaacgaa gttcgcgaag gtcggctcga cgccgggatg
     3361 catcaccgcc ataaactcgt cccagttcgt ccagcggcgg acgaactcgc tgtcaaacca
     3421 gtcgtgctgg atgagctggt tggccagcgc cagcaggacg atcgtgtcgg tgccgggcgc
     3481 cggcgccatc cagtagttcg ccatcgaggc cgtgttggag aggcgcgggt ccatgacagc
     3541 gatcttcgcg ccggcc
//
LOCUS       DASUGJ010000300         3998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1990805, whole genome shotgun sequence.
ACCESSION   DASUGJ010000300 DASUGJ010000000
VERSION     DASUGJ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990805"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFV93_06635"
     CDS             <1..400
                     /locus_tag="VFV93_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5165053.1"
                     /translation="AALKKAVEEMRPYFHQCHRCGNWVCRDVCWNEQAGLCTSCSPKI
                     EQELGALQAEERTRQLQEALTSIDLTAGIDVGRAVVARCPACMAETGGAKFCPDCGTS
                     LAPKTTCSGCGAEARAGTRFCPECGLPMGA"
     gene            546..2417
                     /locus_tag="VFV93_06640"
     CDS             546..2417
                     /locus_tag="VFV93_06640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX5165054.1"
                     /translation="MARRQTAKSTRVDDFRYDATRKNNPPIGLASYEPSLQEPETREY
                     AYDPHLSPQLIWAGKPGLRSIEVEDAAGVEVETVSLHVHERVSSRAIIDAVRRPEPKQ
                     LGLFADPQLPLTEAVEFYEHDVDWTNRLILGDSLYVMNSLAERESLAGKVQRIYVDPP
                     YGVDYKSNFQPRINQRDVKEDDASLSREPEMIKAYRDTWTLGVHSYLTYLRDRLRVAR
                     ELLTDSGSIFVQISDDNVHRVRALLDEVFGAENIIGQVVFQKTGGFGQTAFPSIADFL
                     LWYAKSADHVKVRRLLKPLDDPPANDPNYASLEFPDGTTRRMTREERRRETSLPSDAR
                     VFQYGPLTSDGRSNSPQDFKFEGRPYRPGPNQHWKTSLQGMERLARAGYILRSGENNV
                     RFKLYWDSFAAQRLSNVWLDTQSGGFNDPKIYVVQTTNKVIERCLLMTTDAGDLVLDP
                     TCGSGTSAYVAEEWGRRWITIDTSRVALSLARQRLMTATFPYYQLADREAGVDGGFVY
                     KTVPHITLKSIAQNTRIDPVAERYQQQVDALLAGGQILTRRMCARCGWRRSAKSTPSS
                     PRTPSRRRSTTSRTRSAASFVFPAHSRSRPSRPPSSRSRLSHRLAGSRNRITRLMAS"
     gene            3252..>3998
                     /locus_tag="VFV93_06645"
     CDS             3252..>3998
                     /locus_tag="VFV93_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165055.1"
                     /translation="MSQGSQFMFSRLRRWLLGDDISGETVITDDVPKYVSVRDFVYLD
                     IERVKSLLAQLEQGLLTDRTTTRGSTDSLVGHAGLSVPTIGTVGSSAEYVWQHHASET
                     RTLHDHIFNQTEALLVRHGKVTRFPEDIQDGRLSLEVFRSQISPVGYVIVKGRPTISD
                     YNSLSTMLRDMNQFAGLFAEVQFAKQFSTAASEVERAKVEQQKEASAQDMQQKTGFFQ
                     PFASVIEGFYHDRLILRVFPFDEASVRFAAP"
BASE COUNT          819 a         1296 c         1144 g          739 t
ORIGIN      
        1 cgccgctctg aagaaggccg tcgaggagat gcggccgtat ttccaccagt gtcaccgctg
       61 cggcaactgg gtctgtcgcg atgtctgctg gaacgagcag gccggcctct gcaccagttg
      121 ctcgccgaag atcgagcagg aactgggcgc gctccaggcc gaggagcgta cgcgccagct
      181 ccaggaggcg ctgacgtcga tcgacctcac ggccggcatc gacgtcggcc gcgccgtcgt
      241 cgcccgctgc ccggcctgca tggccgagac cggcggcgcg aaattctgcc ccgactgcgg
      301 cacctcgctc gcgcccaaaa ccacctgctc tggctgcggc gccgaagcac gggccggcac
      361 cagattctgc cccgaatgcg gcctgccgat gggcgcctga accgacaacc cagctcgtag
      421 gggcgagtcg gtgactcgcc tcaatatcaa taatcaatcg cccaatcccg cccccgtcct
      481 tgagcaactc ctgccactgc gttaccatcc cttcgtcaca accggacggc gatggggagg
      541 tgcagatggc tcggcggcag accgcgaagt caacacgcgt ggacgatttc cgctacgacg
      601 ccacgcgcaa gaacaaccct cccatcggcc tcgccagcta cgagccgtcg ctccaggaac
      661 ccgagacgcg cgaatacgcc tacgatccgc acctctcgcc gcaactgatc tgggccggca
      721 agcccggcct gcgctcgatc gaggtcgagg acgcggccgg tgtcgaggtc gagaccgtct
      781 cactccatgt ccacgagcgc gtctcatccc gcgccatcat cgacgccgtc cgccggccgg
      841 agccgaagca gctcggcctc ttcgccgatc cacagctgcc gctgaccgag gccgtcgagt
      901 tctacgagca cgatgtcgac tggaccaacc gcctcatcct cggcgatagc ctctatgtca
      961 tgaactcgct ggccgagcgc gagagcctgg ccggcaaggt ccagaggatc tacgttgatc
     1021 ccccatatgg agtggactac aagagtaact tccagccgag gatcaaccag cgcgatgtca
     1081 aggaggacga cgcctccctc tcgcgcgagc cggagatgat caaggcctac cgcgacacct
     1141 ggacgctcgg cgtccactcc tacctgacct acctgcgcga ccgcctccgc gtcgcccgcg
     1201 aactgctgac cgactccggt agcatcttcg tccagattag cgacgacaat gtgcatcgcg
     1261 ttcgagcgct gttggatgaa gttttcggcg ctgagaacat catcggacaa gtcgtctttc
     1321 aaaagactgg cgggtttgga caaacggcat ttccaagcat tgcagacttt ctcctgtggt
     1381 acgccaagtc agccgatcac gtaaaggttc gtcgcctgct gaaaccactt gatgatcctc
     1441 ctgctaacga tccaaactat gcgagccttg agtttcccga tggtacgacg cgtcggatga
     1501 ccagagaaga acggcggcga gagacatcct tgccatctga cgctcgggtg tttcagtacg
     1561 gcccacttac ttcggatgga cgatccaatt cacctcagga cttcaagttt gagggtcgtc
     1621 cgtatcgccc ggggccgaac cagcattgga aaacgtctct ccaagggatg gagcgtttag
     1681 ccagagcggg gtacattctt cgctcgggag agaacaacgt tcgcttcaag ttgtactggg
     1741 acagctttgc cgcccaaaga ttgagtaacg tctggctaga cacacagtca ggtgggttca
     1801 acgacccaaa aatctatgtg gtccaaacaa caaacaaagt catcgagcgt tgcctcttga
     1861 tgacaacaga tgcaggcgat ctggtgttgg atcctacgtg tggtagcgga acttctgcct
     1921 atgtcgccga agagtggggg cggcgctgga tcacgatcga cacgagccgg gtggcgctct
     1981 cgctggcgcg gcagcggctg atgacggcca ccttcccgta ttaccagctt gccgaccgcg
     2041 aggccggcgt cgatggcggc ttcgtctaca agaccgttcc acacatcacg ctcaagtcga
     2101 ttgcccagaa cacgcgcatc gacccggtcg ccgagcgcta ccaacaacag gttgatgcac
     2161 ttcttgcggg ggggcagatc ctgactcgga ggatgtgcgc gcgttgcggc tggcgcagaa
     2221 gcgcgaaatc gacgccatca tcgccgagga cgccgagcag gagacgctct acgaccagcc
     2281 gcacgaggag cgcggcgtcg ttcgtgtttc cggcccattc acggtcgagg ccatcccgcc
     2341 cgccgtcctc tcgatccagg ctgagtcacc gattggcggg gagccggaac cggatcacga
     2401 ggctgatggc atcgtaaccg ccgccgtcga ggccgacgcc aacgccgacg cggccgtcat
     2461 caaccgcctg atcggcctga tgcggaccga cggcatcacc ttccccaaca acaagcgtct
     2521 gaccttcgac gacctgcgcc ccgtctcggc cggcgtcatc cacgccgagg gcgaggccgc
     2581 cggagacagc acgctcggac gggtcgccat ctcgttcggc ccgcagaacg gcgctgtctc
     2641 ggttcgccag gtcgaggacg gcctctacgc cgcccgccgt gggggctacg acgcgatcgt
     2701 cttcgccggg ttcgccttcc aggccgaggc ccagcaggcc atcagcgaca tgcgccaccc
     2761 gaacctgacc gcccacatgt cccacatccg ccccgacgtc atcatgaccg acagcaaggg
     2821 cgacagcctc ctcaagacga ccgctaactc ccagctcttt gctgtctttg gcgagccgga
     2881 tgtccagttg cacgccgccg ccgatggcaa ctacaccgtc gagctgcacg gcgtcgatgt
     2941 ctacaacccg ctcgacggaa ccgtcgtctc ggcccgcgcc ggccagatcg ccgcctggtt
     3001 cctcgacacc gactacgacg gccgcacctt tgccatctgc caggcgttct tccccgacag
     3061 caaggcctgg gacaagctcg aacgcgccct caaaggcacc ctcgacgccg accgcttcga
     3121 ccaactgacc ggcaccacct cgctcccctt cgtcgccggc caacaccgcc gcgtcgcgac
     3181 gaaagtcatc gaccagcgcg ggaacgaggt catgcgggtg ctggaactcc attcgtgatt
     3241 ggatcaaggg gatgagccaa ggttcacaat ttatgttcag ccgtctaaga cgttggcttc
     3301 tgggcgatga catcagcggc gaaaccgtga taactgatga cgttcctaaa tacgtgtctg
     3361 tccgcgactt tgtttactta gatatcgaac gtgtgaagtc actattagcg caactggagc
     3421 aaggacttct gacagatcga acgaccacgc gaggttcgac ggacagtctt gttggccatg
     3481 ctggattgtc cgttcctacg ataggaacgg ttgggagttc tgcggagtac gtttggcaac
     3541 atcatgcatc cgagacacgt accttacacg accacatctt caatcagact gaggctcttc
     3601 tcgttcggca tggaaaagtg acgaggtttc ccgaagacat ccaggacggt cgactgtcgt
     3661 tagaagtgtt tcgatcacag atttccccgg ttgggtatgt gatcgtaaag ggcaggccga
     3721 ccataagtga ctacaactct ctttctacaa tgctccgcga tatgaaccaa ttcgctggct
     3781 tatttgcaga agttcagttt gctaagcaat tcagtacagc tgcgagcgaa gtggagcgtg
     3841 caaaggttga gcaacaaaag gaagcaagcg cacaagacat gcagcaaaag actggattct
     3901 tccaaccgtt cgcctcggtt atcgagggtt tttatcacga ccggcttatt ctgcgtgtct
     3961 ttccatttga cgaggccagt gtaaggtttg ccgcccca
//
LOCUS       DASUGJ010000301         4288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_600188, whole genome shotgun sequence.
ACCESSION   DASUGJ010000301 DASUGJ010000000
VERSION     DASUGJ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600188"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFV93_06650"
     CDS             <1..659
                     /locus_tag="VFV93_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165056.1"
                     /translation="ATPTRPAPSLDSAPSSVRDAAQRLLRAADTPGKTAFIARALSAL
                     ATIADELDERTLADAGGATSDFLVVVKALQQPEVAAIFQEDDPLLPARLRGLEARQQL
                     LEAEGGTMTVSEVAALLGITRQAVEKRRKAGKLIALSLGRRGYAYPRWQFTSDGKLLP
                     GLENVLCALDEHSAMARTIFMLAPTLRLDGMRPLDAIRQGRIDDARRAALTYLEHGAA
                     "
     gene            700..1260
                     /locus_tag="VFV93_06655"
     CDS             700..1260
                     /locus_tag="VFV93_06655"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX5165057.1"
                     /translation="MERYELPIREVANDWFRVHQTRRGPVYFGTSGNNRFDAPDAEFG
                     VMYVGLDAHCAIVETVGFSAFGRLIAASSVAHLSLARIETARLLRLVDLTADGLIRLG
                     ADARLTTGQYTVSQRWSRAIWHHPERPDGVLYRSRHDPSRLSAALFDRVADSVVLTRS
                     DAFTSASMQQSLRDVFATYGYVVVED"
     gene            complement(1266..2774)
                     /locus_tag="VFV93_06660"
     CDS             complement(1266..2774)
                     /locus_tag="VFV93_06660"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX5165058.1"
                     /translation="MRFFMGGVNHETNTFSPIPASGQRFRDMRYERGAELITRNTGVR
                     NVIGGFIDAARELGIELAPTIHSFAMPSGAVVREEFEAQMADTLDDLEAAMAAGPLDG
                     VLLGLHGAMVIEGIDDGEGEYLRRMREAVGPDMPIVTELDLHANISRESTEIADLIIG
                     YDTYPHIDTYERAVELTHLLARLAKHEIRPVVAFRQIPILANLPAQFTGRKPMSDWVA
                     LCHEIEARPGVLTATVAAGFPYADIPDTSMSVYVATDGNQALADRCADELAQFAWDNR
                     EGFQATPMAIDEAVEYAMTNPGPILLADVADNTGAGASGDGTEVLRSLIAHNARRAVV
                     ALMYDPETVEQAIAAGVGATIDATIGGKVDDKHGAPVQATAYVRAITDGFFYNAGPMS
                     TGEQSSMGKTVVLEIGGRGGIEVVCTQFRRAANDANTLRSAGVEPTQRQIVVIKSSVH
                     YRADFTPLVKEIIEVDAPGLAASNWSHFTFQRLRRPIYPLDGDFDWSPSERE"
     gene            complement(2869..3252)
                     /locus_tag="VFV93_06665"
     CDS             complement(2869..3252)
                     /locus_tag="VFV93_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5165059.1"
                     /translation="MPQKHIAVVNDDTTFLELMEALLSDEGYQTSLMKVTGEAFERIR
                     AIQPDLVIIDIWMQRADNGWEVLNLIRLDPSTAQIPAIVSSTDVRDLEAKAELLRSLN
                     CDVLPKPFNLQNLLDKVNEAIGPPV"
     gene            3417..3635
                     /locus_tag="VFV93_06670"
     CDS             3417..3635
                     /locus_tag="VFV93_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165060.1"
                     /translation="MSERDLIDRLVHQSHYTPDELSHLLGIGRHIIQHAVYAGDLKAY
                     VVDHHCLDIRREDALIWLSTRGLESMPA"
     gene            3968..>4288
                     /locus_tag="VFV93_06675"
     CDS             3968..>4288
                     /locus_tag="VFV93_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165061.1"
                     /translation="MRPIPGGHQRQRVPLLATIAADTISRTGNLLTAIAIPWFVLETT
                     GSPARTGVAVFAGALPVVISLFFGGTLVDRLPYRRVSVVADLASGVSVLLIPFLHAFG
                     LLSFP"
BASE COUNT          706 a         1396 c         1370 g          816 t
ORIGIN      
        1 ctgcaacgcc gaccagaccc gcgccgtcac tcgactccgc gccgtcgtca gtccgtgacg
       61 cggcacagcg gctgctgcgc gccgccgata cgccgggcaa gactgccttc atcgcccgtg
      121 ccttgtccgc cctcgccacg atcgccgatg agttggatga acggacgctg gctgacgccg
      181 gcggtgcgac gtccgatttt ctggtcgtcg tcaaggcgct ccagcagccc gaagtggccg
      241 cgatcttcca ggaggatgat ccattgctac cggcccggct gcgcggcctt gaagctcgcc
      301 agcaactgct cgaagccgag ggtggcacga tgaccgtcag tgaggtcgca gcgctgctcg
      361 gcatcacccg ccaggccgtc gagaagcgcc gcaaggccgg caaactgatc gccctctcgc
      421 tcggccggcg cggctacgcc tacccgcgct ggcagttcac ctccgatggc aagttgctac
      481 cgggactcga gaatgtgctc tgcgccctgg acgaacattc cgcgatggcg cggacaatct
      541 tcatgctggc gccaacactt cggctcgacg gtatgcgacc actggatgcg attcgtcagg
      601 gcagaattga tgatgccagg cgggcggcgt tgacgtatct tgagcatgga gcagcgtagc
      661 gacgagaggc tgggagacca tcctgagcct cctgcggact tggaacgcta cgaactgcca
      721 attcgcgaag tcgccaacga ctggtttcgt gtgcatcaaa cccgccgcgg gcctgtctat
      781 ttcggaacat caggcaacaa tagattcgat gctccagatg ccgaattcgg cgtcatgtat
      841 gttggcttgg acgcgcactg cgcgattgtc gaaaccgttg ggttctcagc gttcgggcgt
      901 ttgattgctg cgtcgtccgt ggcacacctt tcgctcgctc gcatcgaaac ggcccggcta
      961 cttcgcctgg ttgatctgac ggcggatggt ctcattcgac ttggggcgga cgcgcggctg
     1021 acgaccggtc agtacacggt ctcacagcgc tggtcacgcg cgatctggca ccacccggaa
     1081 cgaccagatg gcgtgctcta ccgatctcgt cacgaccctt cccgactgag tgctgcgctc
     1141 ttcgatcgcg tcgctgattc cgtcgtgctg actcgatccg acgcattcac ctcagcatcg
     1201 atgcagcagt cgctgcggga cgtcttcgct acgtatggct acgtcgtggt cgaggactga
     1261 attctttact ctcgctcgct ggggctccag tcgaagtcgc cgtccagcgg gtagatcggg
     1321 cggcgcaggc gctggaaggt gaagtggctc cagttggagg cagccaggcc gggagcatcg
     1381 acctcgatga tctccttgac gaggggcgtg aaatcggcgc gatagtggac cgatgacttg
     1441 atgacgacga tctggcgctg ggttggctcg acgccggctg agcgcagcgt gttggcgtcg
     1501 ttggccgccc gccggaactg tgtgcagacg acttcgatcc cgccacgacc gccgatttcc
     1561 agcacgaccg tcttccccat gctcgactgc tcgccagtgc tcatcggtcc agcgttgtag
     1621 aagaagccgt cggtgatggc ccgcacgtag gccgtcgcct ggaccggcgc gccgtgcttg
     1681 tcgtcgacct tgccaccgat cgtcgcatcg atcgtcgcgc cgacgccggc tgcgatggcc
     1741 tgctcgaccg tctccgggtc gtacatcagc gcgacgacgg cccggcgggc gttgtgcgcg
     1801 atcagcgagc gcagcacctc ggtgccgtcg ccggacgcgc cggcgccggt gttgtcggcc
     1861 acatcggcca gcaggatcgg gccgggattc gtcatcgcgt actcgactgc ttcgtcgatc
     1921 gccatcggtg tcgcctggaa tccttcccgg ttgtcccagg cgaactgcgc cagctcgtcg
     1981 gcgcagcggt cggccagcgc ctgattgccg tcggtcgcga cgtagacgct catgctggtg
     2041 tcgggaatgt cggcgtaggg gaaaccggcc gcgacggttg ccgtcaggac gccgggccgc
     2101 gcctcgatct cgtggcagag cgcgacccag tcggacatcg gcttgcggcc ggtgaactgg
     2161 gccggcagat tggccaggat cgggatctgg cggaaggcga cgaccggtcg gatctcgtgt
     2221 ttcgccagcc gtgccagcag gtgagttagc tcgacggccc gttcgtaggt gtcgatgtgc
     2281 gggtaggtat cgtagccgat gatgaggtcg gcgatctcgg tcgattcgcg ggagatgttg
     2341 gcgtgcaggt cgagctccgt cacgatcggc atgtccggcc cgaccgcctc gcgcatccgc
     2401 cgcaggtact cgccctcgcc gtcatcgatc ccttcgatca ccatcgcgcc gtgcagcccg
     2461 agcaggacgc cgtcgagcgg gccggccgcc atcgctgcct ccagatcgtc cagcgtgtcg
     2521 gccatctgcg cctcgaattc ctcgcggacg acggcaccgg acggcatcgc gaacgagtgg
     2581 atcgtcggcg ccagctcgat ccccagttcg cgggcggcgt cgatgaaacc gccgatcacg
     2641 ttgcggacgc cggtgttcct cgtgatcagc tctgcgccgc gctcgtagcg catgtcgcga
     2701 aagcgctgac cgctggctgg gatcggcgaa aatgtgttgg tttcgtgatt gacaccaccc
     2761 atgaagaatc gcatacctgc cccgcctctc acccatcgct ccaccgtcgt cctggcgcgg
     2821 cgatggtacg tggacacggc ggacgcggca agccccggcg gtgtcgcgct acaccggcgg
     2881 gccgatggct tcgttgactt tatcgagcag gttctggaga ttgaacggct tcggcaagac
     2941 gtcgcagttg agcgagcgca ggagttccgc tttcgcttcc agatcgcgaa cgtcggttga
     3001 tgacacgatc gccggaatct gcgccgtcga tgggtcgagc cggatcaggt tcagaacctc
     3061 ccagccgttg tcggcgcgct gcatccagat gtcgatgata accaggtccg gctggatcgc
     3121 cctgatccgc tcgaatgcct cacctgtcac cttcatcagt gacgtctgat agccctcgtc
     3181 cgagagaagc gcttccatca gttccagaaa ggtggtgtcg tcattgacga ccgcgatatg
     3241 tttctgcggc atatgttccc cttccaggct ggaaaacgca caatacaggc cgcgcccgcc
     3301 ggaagcggca cggttgccct tgcgcccgat ggcattcggc gtagaatgaa gttatgtcgt
     3361 gccgaatcgc atcagaccga caggagggct gcaagatgga tgaacgaggg cagccgttgt
     3421 ccgaacgtga tctgatcgac cgtctcgtgc accagagcca ctacaccccg gatgaactgt
     3481 ctcatctgct gggaattggc aggcacatca ttcagcacgc cgtctatgcc ggcgatctga
     3541 aggcgtacgt cgtcgatcac cactgtctcg atatccgccg cgaagacgcg ctcatctggc
     3601 tcagcacacg cggtctcgaa agcatgcccg cataggccag acgccgttcc caacatgccc
     3661 caacgtcaac gacgcgcacc tgttcgccgt cagccattgc tgtgccaatg ccggttgacc
     3721 cgcgcgcatt cgaccacgga taatccagtg acaaatcagg ccgcgtgtct gcactccacc
     3781 ctggtagcag ggtgatcaga gctaccggaa accgcaagga gaccggccgg cgagaccgat
     3841 cggcgcggtg accaccgaac caactgtgga cggtggagga agtctctccc ttggcggtag
     3901 ggcgagcggc ttccttcgcc gtcccctgtt tctgacgccg gatcggaggc cgcaacagga
     3961 ggcattgatg cggccaattc ccggcggaca ccagcggcaa cgagtgccgc tactcgcgac
     4021 gatcgccgcc gataccatct cgcggactgg gaacctgctg acggcgatcg cgatcccctg
     4081 gttcgtgctg gagacgactg gcagccccgc gcggaccggc gtggccgtct tcgccggggc
     4141 gctgccggtc gtcatctcgc tcttcttcgg cgggacgctc gtcgatcgct tgccctaccg
     4201 gcgcgttagc gtcgtcgccg acctggccag cggggtgtca gtgctgctga ttccgttcct
     4261 gcacgcgttc gggctgctct cgttcccg
//
LOCUS       DASUGJ010000302         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_879946, whole genome shotgun sequence.
ACCESSION   DASUGJ010000302 DASUGJ010000000
VERSION     DASUGJ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879946"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..>2942
                     /locus_tag="VFV93_06680"
     CDS             299..>2942
                     /locus_tag="VFV93_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX5165062.1"
                     /translation="MTRWVVDRNGDGFPDDCAVRLVVDAGDQIERDVVSALLDLAARV
                     GLETHALPFPLVVGDVAAVPAGVDSVVIHERGDIPAISGSDAQPGGSIPAVSDPPAGC
                     LTHLFTLDGLLEDRDGDLLPDASRVAFDLPEALPAGLAVALANLAARIGLESGGLTFP
                     LVRDQGASFVVRPGSGSAILRALDGGWLAEGDAGDLARLIERVAATWPHIDAPQTGGA
                     GSALARLRRWLAGDGPEPNEPGEVIFERDWTADWEGDRLVSHVEEVLAQMADEVDSDY
                     RDPDDLHEPLIVFACEPPEQRRQIKAAIEQVVAASPFTGLDITVLSAFKTGLSWLREV
                     VIPELTGLPLSRVRVEAMAHTGEYLEDDETVLDMQIRWLQELFPGNELISEATGLAPD
                     AIEFVVSDDALATFVAEAFDEADELLGRWECEIPSQYQPFIAALPDAGLVRVTTAGTT
                     WYRDGEYDATLIVPTDLDTFWTFWQDEIIPAIFAHIDAAGGPSAAGQPFFGELLAEVW
                     ISAPNERLGIREENDSAAEALAEDIYFTTLDAIELYGRQQTGERCNAPGAVIPIVHVT
                     PGQPPRARVTLRAAPTRRELPRPDLRVAGLRLDGDELVAEVKAAVNGDTGPTIARLSE
                     LAGLPASAGRSLAARVTLAGTTVDMRLPLPAVLRAEASSPAPPPMTENIHGDAVIEQA
                     ARLTAFPEVTAWIEDVSYQGRPLVALALAAPTPGRLVSTMKSVIFKPTHLIIARHHAN
                     EVSSTNAAFQLAWLTATDPDWRSYLDRVNVVLLPYENPDGAALHVRLAAYPEARTWKH
                     HPARYNALGYEFGEAHFDPDTRIGEARARTALWQRWPADVVVDNHGVPSHEWVQPFAG
                     FGSPPRFPMSYWIVQALLYGIVRYV"
BASE COUNT          435 a          936 c         1038 g          533 t
ORIGIN      
        1 ggtcagtgct gccccgctcc accctcagtc tcacagagag tcatcctgag ctgcagcgaa
       61 ggatcgctca acgtgggaca ttaggagcgc gccaatcggt agcagagcgc atcggtcctc
      121 gttggccgcg tacgtcgtta cggttcagcg atccttcgct gcggctcagg atgacagcgg
      181 tggcgcgtgc ggtccttgcc ggcgccgtac ggctggatcg cgtcacgcgt cacgcatctc
      241 gcgggtaaga tgcacggatt ggttgtcgtg cggcaggcgg aggtggacgg tggatgacgt
      301 gacgcggtgg gtcgtcgatc ggaatggcga tggatttccg gacgactgtg ctgtccggct
      361 ggttgtcgac gctggcgatc agatcgagcg cgatgtcgtc tccgcgttgc tcgacctcgc
      421 ggcccgggtt ggtcttgaga cgcacgcgct gccattccca ttggttgttg gcgatgtcgc
      481 ggcggttccg gccggcgtcg attcagtcgt catccacgag cgtggcgata ttccggcgat
      541 ttccggaagc gacgcccagc ctggcgggag tatcccggca gtcagcgatc cgccagccgg
      601 ttgcctgacg cacctgttca cgctggatgg tctgctggag gatcgcgatg gcgacctgct
      661 gccggatgcg agccgggttg cgttcgatct gccggaggcg ctaccggccg ggctggcggt
      721 ggcgctggcg aatctggcgg cgcggatcgg gctggaatcg ggtggcctga ccttcccgct
      781 ggtgcgcgat cagggcgcgt cgttcgtcgt ccgtcccggc agtgggtccg cgatactgcg
      841 ggccctcgac ggcggctggc tggctgaagg ggatgcgggc gatctggcgc ggttgatcga
      901 gcgggtggcg gcgacctggc cgcacatcga cgcaccgcag acgggcgggg caggctcggc
      961 gctggcgagg ctgcgccgct ggctggccgg tgacggcccg gagccgaatg agccgggcga
     1021 ggtgatcttc gagcgcgact ggaccgccga ctgggagggc gaccggctcg tcagccacgt
     1081 cgaggaggtc ctcgcgcaga tggcggacga ggtcgattcc gactatcgcg atccggacga
     1141 tctgcacgag ccactgatcg tcttcgcctg cgagccgccg gagcagcgcc ggcagatcaa
     1201 ggctgccatc gagcaggtgg tggctgcctc accgtttacg ggtctcgaca tcaccgtcct
     1261 ctctgcgttc aagaccgggc tgtcttggtt gcgcgaggtc gtgattccgg aattgaccgg
     1321 cctgccgctc agccgggtgc gcgtcgaggc gatggcccat acaggcgagt atctggaaga
     1381 tgatgagacc gtcctcgaca tgcagatccg ctggctccag gaactgtttc ctggcaacga
     1441 actgatctcc gaagcgaccg gactggcgcc ggatgcgatc gagttcgtgg tttcggacga
     1501 tgcgctcgcg acatttgtcg ccgaagcgtt cgatgaggcc gacgagctgc tgggacgctg
     1561 ggaatgtgag attccgagcc agtaccagcc gtttattgcc gcgctgccgg atgccgggct
     1621 ggtccgggtg acgacggccg gtacgacctg gtatcgagat ggcgaatacg acgcaacgct
     1681 gatcgtgccg accgacctgg acacgttctg gacgttctgg caggatgaga tcatcccggc
     1741 catcttcgcc cacatcgacg cggcaggtgg gccatccgct gccggtcagc cgttcttcgg
     1801 cgaactgctg gctgaggtct ggatcagcgc gccgaacgag cggctcggca tccgcgagga
     1861 gaacgactcg gccgccgaag cgctggctga ggatatctac ttcacgaccc tcgacgcgat
     1921 cgagctttac ggccgccagc agaccggcga gcgctgcaac gcgcccggcg ccgtgatccc
     1981 gatcgtccac gtcacgcccg gtcagcctcc acgcgcgcgg gtgacgctcc gcgccgcccc
     2041 gacccgccgc gagcttccac ggccggacct gcgcgttgcc ggattgcgtc tcgatggcga
     2101 tgagttggtt gcggaagtta aagcagccgt taacggcgat acgggcccga cgatcgcgcg
     2161 tctttccgag ttggctggtt taccggcgtc ggccggtcga tcgctcgcgg cccgcgtgac
     2221 gctggccggc acgacggttg atatgcgtct tccgctgccg gccgtgctcc gtgcagaggc
     2281 gtcgtcaccg gcaccgccgc cgatgacgga gaacatccac ggtgatgcgg tcatcgagca
     2341 ggcggcgcgg ctgaccgcct tcccggaggt gacggcctgg atcgaggatg tgtcgtatca
     2401 ggggcgaccg ctggttgcgc tggcgctggc cgcgccgacg ccggggcggc tggtgtcgac
     2461 gatgaagtcg gtcatcttca agccgacgca cctgatcatc gcgcgccacc atgccaacga
     2521 ggtctccagc acgaacgccg ccttccagct ggcctggctg accgcgaccg atcccgactg
     2581 gcggagttat ctcgaccgcg tcaacgtcgt cctgctgccg tatgagaatc cggacggcgc
     2641 tgcgctgcat gtccggttgg cagcgtatcc cgaggcgaga acgtggaagc accatccggc
     2701 ccgctacaac gcgctcggct acgagttcgg cgaggcgcac ttcgatcccg acacgcgcat
     2761 cggcgaggcg cgggcacgaa cggccctctg gcagcgctgg ccggccgatg tcgtcgtcga
     2821 taaccacggc gtgccgtcgc atgagtgggt ccagccgttc gccggtttcg gctcgcctcc
     2881 ccgcttcccg atgtcctact ggatcgtcca ggcgctcctc tacgggatcg ttcgctatgt
     2941 cg
//
LOCUS       DASUGJ010000303         6384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1433931, whole genome shotgun sequence.
ACCESSION   DASUGJ010000303 DASUGJ010000000
VERSION     DASUGJ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433931"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..2097
                     /locus_tag="VFV93_06685"
     CDS             376..2097
                     /locus_tag="VFV93_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX5165063.1"
                     /translation="MSWWYSLILAVPLIVLFAMALTSGSDDDDGLTGVVRDAFTGDPV
                     SGAVVSTANATATTNGDGAFTVADLTATSLFISREDYASTQVAVTSPDIEYDVSLRPT
                     TVHGTVKNEKNGDPIAGVTVTATAPDGTSITTQTNEDGEYTLENVPDGAEVTVTFEGF
                     TVATLPLGSDANLDFKIRPDVLTGLVTDEEGKPVAGASVSVGTATTTTNADGTYRVAG
                     IGEDAKITIRKPGFLDVTADVPKSLTYDTTMTRFLVKAIYSTAQYAASDELWGSLIDI
                     ADTTEVNAMVLDLKDSSGNVFYDTKVPLATQIGAKKPMYDINERLQEMKDHNVYSIAR
                     IVVFEDPILAGARNELAIHDVTTGGLWTTWDGLAWVNAHQREVWQYNIDLAVEAAELG
                     FDEIQLDYIRFPSDGMLENADYGSQFANETRVDAITGFLTQMQSALKPTSSLLAVDIF
                     GITLLEDDDAGIGQHFASIVPLVDVICPMIYPSHFYPGEMGFDIPNNHPYDVIMQSLQ
                     RGVEMVPDSRDKLRPWLQDFSYGEGIKYGAPEVAAQIQATVDFGTAGWLIWAPDNVYS
                     VDAFSPE"
     gene            2157..3074
                     /locus_tag="VFV93_06690"
     CDS             2157..3074
                     /locus_tag="VFV93_06690"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEX5165064.1"
                     /translation="MTIDRQPLAPARLVDRSGLWPYLAAMRPKQWTKNAVVFAALVFD
                     LKLFDLSRFATVVGAFFCFCLASSAVYIVNDLRDAESDRQHPKKRLRPIASGAISSQS
                     AWLLVVLLLVVILPVAVMLRPAFAGVLVGYLALMTVYTLVLKHLVIVDVFAIAAGFVL
                     RAAGGAVVLDVPISPWLYVCTVLLSLFIGFGKRRHELLLLDTDAAAHRRNLDEYSAEL
                     LDQFITITAAATMMAYSLYTFEADTLPDNSTMMLTIPFVIYAIMRYLFLVHRRDGGGS
                     PEQALLTDLPLLSCIALWGIVAIVILYAG"
     gene            3230..3685
                     /locus_tag="VFV93_06695"
     CDS             3230..3685
                     /locus_tag="VFV93_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5165065.1"
                     /translation="MYVVRKGGNRNIERIQHEMEDVFNALMSSGRPIRVRIAPGSTPA
                     WRPPIEVYETDDALVALIEIAGMSEDEIEVVLDDSVMTVRGNRRPAICEDRRTVHAMG
                     ILYGPFAAEVYLPFSVDPEGVAATYEAGILRVRLPRAAVTRISIGSNGR"
     gene            3752..6268
                     /gene="lon"
                     /locus_tag="VFV93_06700"
     CDS             3752..6268
                     /gene="lon"
                     /locus_tag="VFV93_06700"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5165066.1"
                     /translation="MADENTNVNEEQTLAPDIETGSESPANETATDTVTSASLPVLPL
                     RATVVFPMTVVPLAAAQPRSIRLIDAVASGDRTVVLVLQNDAEQEGAGPGETAMIGTL
                     ATIHQMMRVPDGSVRLAVQGLKRVRIDRWVSDDPYLTAEVTEIPETEEDDIETKALMR
                     STVELFQRLVSLVSNLPDELVTAALNIDEPLQLVYLIATNLRMEPEERQQLLELNSVR
                     EKLEQISRFMAKELDLLELGKKIQGEVQEEVGRNQREYYLREQLKAIQKELGESDGQE
                     AEVTALREQIENAGMPEEALREANRELDRLSKLPPAAAEYGVIKTYLDWLVSMPWNVS
                     TESDIDVKAAREILDRDHYNMEKVKDRILEYLAVHRLRNERIAQGETIEGREPILCFV
                     GPPGVGKTSLAQSIADAIGRKFTRMSLGGVRDEAEIRGHRRTYIGAMPGRIIQAIRRA
                     GANDPVFVLDEVDKLGADWRGDPSSALLEVLDPEQNDTFRDHYLDVPFDLSKVMFITT
                     ANLLDTIPAPLRDRMEIIQLSGYTDEEKLQIAKRYLVPRQLRANAIPDLPIEWDDESL
                     LNIIQGYTRESGVRNLEREIGTVARKLATKQAAGDTLPERVTPDVVREYLGRPRFFYE
                     ELETRTSLPGVAIGVGVTAVGGDIMFIETSKSEGRGNVTITGQLGDVMRESAQAAMSF
                     VRSRASDLGIDSSVFKDVDIHVHVPAGATPKDGPSAGVALTTALVSLLTGIPVREDVA
                     MTGEVTLRGQVLPVGGIKEKSLAAHRAGVTTFILPKRNEADLDDLPANLKDALRFVPV
                     ETIDDALAVSLPDEFHMRRQTPDDFAARQSGERIAARASA"
BASE COUNT         1242 a         1915 c         2025 g         1202 t
ORIGIN      
        1 tccaccgttg tgcgtgtcct gtccgtttgg gttcccgatc tgtcgcccgg gcgcgatgca
       61 ggtgaacggt ggaagaatcc gccagagatt ccccacatgg gcgtgaagta tacgaaggtg
      121 gctaccagcg ggcaatttac cccgccatcc ggcggtttcg tcgccgcctg agtcgcgttg
      181 tacactagcg gacgttgggg gcgtcgaaac acgctggcct gataccggta tgacatgggc
      241 agtccagaat cccgttgcgc ggatgcgccg cgatatgcga ttgcgctgta aggcgcacag
      301 gatccggttg gctcaggagg ggcttggttg attccagatc gcaacattcg tgaacggcgc
      361 cggccatggg atcaagtgag ctggtggtat agcctgatcc tggcagtccc actcatcgtc
      421 ctcttcgcga tggcgttgac gtccggttcc gacgacgatg acggtctgac tggagtcgtg
      481 cgcgacgcat ttaccggcga tccggtttct ggcgcggttg tgtcgacagc gaacgctacc
      541 gcgacgacca acggtgatgg cgcatttacc gtcgccgatc tgacggccac ctcactcttc
      601 attagtcgcg aagactacgc cagtacccaa gttgccgtta cctccccaga catcgaatat
      661 gacgtctcgc tgcggccgac gacggtccat ggcacggtca agaacgagaa gaacggtgat
      721 ccgatcgcgg gcgtcacggt gacggccacc gctccagacg ggacgtcgat cacaacccag
      781 acaaacgagg atggcgagta cacgcttgag aacgtgccgg atggcgctga agtaaccgtc
      841 accttcgaag gattcacggt cgcgactttg ccgctgggtt cagacgctaa tctggatttc
      901 aagatccggc ccgacgtgct gactggactc gttaccgatg aagaaggaaa acctgtcgcc
      961 ggtgcttccg tctcggtcgg aaccgcgacg acgacgacga acgccgacgg cacgtatcgc
     1021 gtcgccggca ttggcgaaga cgcgaagatc acgatccgga agcccggctt tctggatgtc
     1081 acggccgacg taccgaagtc gctcacgtac gacacgacga tgacccgctt ccttgtcaag
     1141 gcaatctact cgaccgcgca gtacgcagcg tcggatgagc tatggggcag tctcatcgac
     1201 atcgccgaca cgaccgaggt gaacgcgatg gtccttgacc tgaaggactc gagcggcaac
     1261 gtcttctacg acacgaaggt gccgctggct acccagatcg gcgcgaagaa gccgatgtac
     1321 gatatcaacg agcgactcca agagatgaag gaccacaacg tctactcaat tgcccggatc
     1381 gtcgtgttcg aggatcctat tctggctggt gcgcgtaacg agttggcgat tcatgacgtg
     1441 acgaccggcg ggctttggac gacctgggac gggcttgcct gggtcaacgc acatcagcgt
     1501 gaggtctggc aatataacat tgacctggcg gtagaggcag ccgaacttgg attcgatgag
     1561 atccagctcg actacatacg ctttccatcc gacggcatgc tcgagaacgc cgactacggc
     1621 tcgcagttcg ctaacgagac acgcgtcgac gcgatcacag gcttcctgac gcagatgcag
     1681 tccgcgctca agccgaccag ttcattgctg gccgtcgata tcttcggcat cacactgctc
     1741 gaagatgatg acgcgggcat cgggcagcac ttcgcgtcaa tcgtgccgct ggtggatgtg
     1801 atctgtccga tgatctaccc gtcgcacttc taccccggcg agatgggctt cgatatcccg
     1861 aacaaccatc cgtacgacgt catcatgcag agcctgcaac ggggtgtgga gatggtgccc
     1921 gattcgcgcg ataagctgcg cccctggttg caggacttct cctatggcga aggcatcaag
     1981 tacggcgcgc ccgaggttgc cgcccagatc caggcgactg tcgactttgg caccgccggc
     2041 tggttgatct gggcgccgga caacgtctac tcagttgatg cgttctctcc ggagtaataa
     2101 cgaccgtcga cggccgggcg ctgccgaacc gagccacatg tgacagcgag gagtttgtga
     2161 ccatcgatcg ccagccgctc gctccggcca gactcgtcga caggtcggga ctctggccgt
     2221 atctggctgc gatgcgtccg aagcaatgga cgaagaacgc cgtcgtcttc gcggcgttgg
     2281 tcttcgacct caagctcttc gatctgagcc gctttgcgac cgtcgtcggg gcgtttttct
     2341 gcttctgcct cgccagtagt gctgtctaca tcgtcaacga cctccgcgac gccgagtcgg
     2401 atcggcagca tccaaagaag cggctgcgtc cgattgcttc gggcgccatc agcagccagt
     2461 cggcctggct gctggtcgtt ctgctgctgg tcgtgatcct tccggtggcc gtgatgttgc
     2521 gaccagcgtt tgcgggcgtg ctggtcggct acctggcgct gatgacggtt tacacgttgg
     2581 tgttgaagca tctcgtcatc gttgatgtct tcgcgatcgc ggccggattc gtcctgcgtg
     2641 ctgctggtgg ggcggtcgtc ctcgacgtcc cgatcagccc gtggctgtac gtctgcaccg
     2701 tcttgttgtc gttgttcatc gggttcggca agcggcgtca cgagttgctg ctgctggaca
     2761 cagacgcggc ggcgcaccgg cgcaatctgg acgagtattc ggccgaactg ctcgaccagt
     2821 tcatcacgat cacggcggcg gcgacgatga tggcctattc actgtatacg tttgaggcag
     2881 acacgctgcc ggacaacagc acgatgatgc tgacgatccc gttcgtgatc tacgcgatca
     2941 tgcgctacct gttcctcgtc catcggcgcg atggtggcgg ctcgcccgaa caggcgctgc
     3001 tgactgacct gccgctgctc agttgcatcg ccctctgggg tatcgtcgct atcgtcatcc
     3061 tctacgccgg atgaactgat acatgtagct tcggcaacct tgagtcgatc tttgtcaact
     3121 ttgtacgcac atgctagaat agagcatgct tccgcaaacg gcggaggtcg atggccggac
     3181 atcttgcccg caggcccgaa ccgggcccaa cacactgagg acaatcacca tgtatgtagt
     3241 tcgcaagggc gggaacagga acatcgaacg catccagcac gaaatggaag acgtcttcaa
     3301 tgcgttgatg tcatcgggac ggccgatccg ggttcggatc gcgccggggt cgacgcccgc
     3361 ctggcgtccg ccgattgaag tctatgagac cgacgacgcg ctggtcgcgc tgatcgaaat
     3421 agcgggtatg tccgaagacg agatcgaagt cgtcctcgat gactcggtca tgaccgttcg
     3481 aggcaaccgg aggccggcga tctgcgagga tcggcgcacg gtccatgcga tgggcatcct
     3541 gtatggtcca ttcgcggccg aggtctacct gccgttcagc gtcgatcccg aaggtgttgc
     3601 agcgacgtat gaggccggga ttctgcgcgt gcgactacca cgcgcagcag taaccagaat
     3661 ctcgatcggg agcaatgggc ggtaaccagt tacctttcgg ccctgagcag tgccccacca
     3721 gacccggtat tcgatgaaga aggagccagt agtggctgac gaaaacacaa acgtgaacga
     3781 agagcagacg ctcgcacccg atatcgagac cggcagcgag tcaccagcga acgagaccgc
     3841 gactgacacc gtgacctcgg catcattgcc ggtgctgccg ttgcgggcaa ccgtcgtctt
     3901 ccctatgacc gtcgtgccgc tggcggccgc ccagccccgg tcgatccggc tgatcgatgc
     3961 tgtcgcttct ggcgaccgca cggtcgtgct cgtgctgcaa aacgacgccg agcaggaggg
     4021 cgccggaccg ggcgaaacgg cgatgatcgg cacgctcgcg actatccacc agatgatgcg
     4081 tgttccggac ggcagcgttc ggctggccgt ccagggcctg aagcgcgtcc gtattgaccg
     4141 atgggtgtct gacgacccgt atctgacggc ggaagtgacc gagattccag agaccgagga
     4201 agacgacatc gaaaccaagg cgctgatgcg ctcgacagtc gagctcttcc agcggttggt
     4261 gagcctcgtc tcgaatctgc cggacgaact cgtgactgcg gcgctcaaca tcgatgagcc
     4321 gcttcagctg gtctacctga tcgcgaccaa cctccgaatg gagccggaag agcgccagca
     4381 gttgctggag ctgaactcgg ttcgagagaa gctcgagcag atctcgcgct tcatggccaa
     4441 ggaactcgac ctgctcgaac tgggcaagaa gatccagggc gaggtccagg aagaggtcgg
     4501 ccgcaaccag cgcgagtact acctgcgcga gcagttgaag gccatccaga aggagctcgg
     4561 cgaatcggac ggccaggagg ctgaggttac ggcgctgcgc gagcagatcg agaacgctgg
     4621 catgccagag gaggcgctgc gcgaggcgaa ccgggagctc gatcggctct cgaagcttcc
     4681 accggctgcc gctgagtacg gtgtcatcaa gacctatctc gactggctcg tctcgatgcc
     4741 gtggaatgtc tcgaccgaat cggacatcga cgtgaaggct gcccgcgaga tcctggaccg
     4801 cgaccactac aacatggaga aggtcaagga ccgcatcctt gagtacctag ctgtccatcg
     4861 gctgcgcaac gagcgcatcg cgcagggaga gacgattgaa gggcgtgagc cgatcctctg
     4921 cttcgtcgga cctccgggcg tcggcaagac ctcgctggcg cagtcgatcg cagacgcgat
     4981 cggccgtaag ttcacccgga tgtcgctggg tggtgtgcgc gatgaggcgg agatccgcgg
     5041 tcaccggcgc acctacatcg gcgcgatgcc gggtcggatc atccaggcaa tccgccgggc
     5101 aggcgcgaac gatccagtct tcgtcctgga tgaggtcgac aagctcggcg ccgactggcg
     5161 cggcgatcca tcctcggcgt tgcttgaggt gctcgatccg gagcagaacg acaccttccg
     5221 cgatcactat ctcgacgtgc cgttcgatct gagcaaggtg atgttcatca cgaccgccaa
     5281 cctgctggac acgattccgg cgccgttgcg cgaccggatg gagatcatcc agctctcggg
     5341 ttacaccgac gaagagaagc tgcagatcgc caagcgctac ctggtgccgc gacaattgcg
     5401 cgccaacgcg attcccgatc tgccgatcga atgggacgac gagtcgctgt tgaacatcat
     5461 tcagggctac acgcgcgaga gtggtgtgcg taacctggag cgtgagatcg gaacggttgc
     5521 tcgcaagctg gcgacgaagc aggctgccgg cgacacgttg cccgaacggg tgacgccgga
     5581 tgtcgttcgc gaatatctgg gccggccgcg ctttttctac gaggagcttg aaacacgcac
     5641 gtcgctgcct ggcgtcgcga tcggcgtcgg cgtgacggcg gttggcggcg acatcatgtt
     5701 catcgagacc tcgaagagcg agggccgcgg taacgtgacg atcaccggtc agcttgggga
     5761 cgtgatgcgc gagtcggcgc aagcggcaat gtcgttcgtt cgctcgaggg caagtgatct
     5821 gggtatcgac tccagcgtct tcaaggacgt ggatatccat gtccacgttc cggccggcgc
     5881 gactcctaag gacggcccat cggccggcgt cgcactgacg acggcgctgg tgtcactgct
     5941 gaccggcatc ccggttcgcg aggacgtcgc gatgaccggt gaggtgacgc tacgcgggca
     6001 ggtgctaccg gtcggtggga tcaaggagaa gtcgctggct gcccatcgag cgggtgtgac
     6061 gacgttcatc ctgcccaagc gcaacgaggc cgacctcgat gacctgccgg cgaacctgaa
     6121 ggacgcgctc cgcttcgtcc cagtcgagac gattgacgac gcactcgcag tttcgctccc
     6181 agacgagttc cacatgcgcc ggcagacacc ggacgatttc gccgcccgcc agagcggtga
     6241 gcggatcgca gcccgggcca gcgcataatg tgacaggcac agacgtacac gacgacggcg
     6301 gccgaactcg gccgccgtcg ttgtgtggtc ggtgaagtcc atcggcgatc gcacaatacg
     6361 tccagccggt aggggcgagg cact
//
LOCUS       DASUGJ010000304         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_324053, whole genome shotgun sequence.
ACCESSION   DASUGJ010000304 DASUGJ010000000
VERSION     DASUGJ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324053"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VFV93_06705"
     CDS             <1..1207
                     /locus_tag="VFV93_06705"
                     /inference="COORDINATES: protein motif:HMM:NF004936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX5165067.1"
                     /translation="YQTDFARNWSKENKHISAMFNEAVQGALQATKIDPKDVEVGHVG
                     NFAAELYTMQGHLGAFLLEADPAFSGLPTGRHEAACASGSIAILAASAEIEAERYDLA
                     MVLGVEQMKTVDSGRGGDFLGTAAWYDREAQGISYPFPKLFGRLGDEYDKRFGLKDEH
                     LARISAINYGNAKKNPWAQTRGWYMSEDHANSQDRFNQIIGGRIKVSDCSQVTDGAIC
                     IFLASKRYAEKYAKAHDLDLNRIPQILGWGHHTAPLGFDEKVAESKDNPFVLPHTRQA
                     IVDALRRAKIADVWDLDAIETHDCFTTSEYMAIDHFGLTPPGKSWQAIEEGVIDLDGQ
                     LPINPSGGLIGAGHPVGGTGVRQVLDAYRQVTDTAGGYQVSGAKKVATLNIGGSGTTS
                     VSFVVGTPA"
     gene            1668..>2047
                     /locus_tag="VFV93_06710"
     CDS             1668..>2047
                     /locus_tag="VFV93_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165068.1"
                     /translation="MRLLVVEGIELRVLDEVAQDVATEALDPEIAELLVDDIGRQMRL
                     LLESGVAHAMDPRPLVRPSFVLAARHGITLYDAQTVILAETTRLPLLVADRDQYGVLQ
                     RIATGRTMLRLLWLPQLLDDLDLS"
BASE COUNT          369 a          656 c          666 g          356 t
ORIGIN      
        1 ataccagacg gattttgctc ggaactggag caaagagaat aagcacatct ccgcgatgtt
       61 caacgaggct gtgcagggcg cgttgcaggc gaccaagatc gacccaaagg atgtcgaggt
      121 cgggcacgtc ggcaacttcg ccgccgagct gtacacgatg caggggcatc tcggcgcgtt
      181 cctgctggag gcggacccgg cgttctccgg cctgccgacc ggccggcatg aggcggcctg
      241 cgcctccggc tcgattgcga tcctggctgc ctcggcggag atcgaggccg aacgctacga
      301 cctggcgatg gtgctcggcg tcgagcagat gaagacggtc gattccggcc gcggcggcga
      361 cttcctcggc acggccgcct ggtacgaccg cgaggcgcag gggatctcct atcccttccc
      421 gaagctgttc ggccggctcg gcgatgagta cgacaagcgg ttcggcctga aggacgagca
      481 cctggcccgc atctccgcga tcaactacgg caacgcgaag aagaatccgt gggcgcagac
      541 gcgcggctgg tacatgagcg aggatcacgc caacagtcag gatcggttca accagatcat
      601 cggcggccgg atcaaggtct ccgactgctc ccaggtgacc gacggcgcga tctgcatctt
      661 cctcgcctcg aagcggtacg ccgagaagta cgccaaggcg cacgacctcg acctgaaccg
      721 gatcccgcag atcctcggtt ggggccacca caccgcgccg cttggcttcg acgagaaggt
      781 ggccgagagc aaggacaacc cgttcgttct gccgcacacc cgccaggcga tcgtcgacgc
      841 gctccgccgc gccaagatcg ccgatgtgtg ggacctcgac gcgatcgaga cgcacgactg
      901 cttcacgacc tcggagtaca tggcgatcga ccacttcggc ctgaccccgc cgggcaagtc
      961 ctggcaggcg atcgaagagg gcgtgatcga cctcgacggc cagctgccga tcaacccgag
     1021 cggcggcctg attggcgcgg gccacccggt cggtggcacc ggcgtccgcc aggtactcga
     1081 tgcctaccgt caggtgacgg acacggcggg cggctaccag gtgagcggcg ccaagaaggt
     1141 cgctacgctc aacatcggcg gctccggcac caccagcgtg tcgtttgtgg tgggcacacc
     1201 ggcgtaacga aaccctcatg cgtgggccgg cgcaccgttc aacggcgggc gccggccgcg
     1261 agggaagcag cgatggcggc agatcgcagg ccgacccgcc gtgcggacgg tatagccgaa
     1321 ggtgacgctt cgaagtcgac cattgaggac gctactcaag cgtatcttga tcgggagatt
     1381 gcacgccttc gccgaacggt agtggtacct tcgttcgtcc cacttacgga tacggacttg
     1441 gcggcgctcc attccggcat cgacgaacat caacgtgtgg tcgcgttggt ggagcagcac
     1501 gtcgcggaac agcgacgtgt cggatttcca cctagagaag gcggttgact gggccgtatg
     1561 cggtggccgt cctgcatccg agcgtggcgg ttcgttggtg gctccgtcat cttccggtgg
     1621 ccgcggagtc aaccaggttt ctccttaatg tgcttgcctc ggggagcatg cggctcctcg
     1681 ttgtcgaggg gatcgagttg cgagtcctgg atgaggtggc acaagacgtc gcgactgaag
     1741 ccctcgaccc ggagattgct gaactactcg ttgacgatat cggccggcag atgcggttac
     1801 tgttggagtc tggcgtagca cacgcaatgg acccgcgccc gctcgtccgg ccatcgttcg
     1861 ttctggctgc ccgtcatggc atcactctct acgatgccca aacggtcatt ctggcagaga
     1921 cgacacgtct acccctgctc gttgccgacc gcgatcagta cggcgtgttg cagcgcatcg
     1981 ctaccggacg caccatgctc cgactcctct ggctgcctca acttctcgat gacctcgatc
     2041 tctcgtg
//
LOCUS       DASUGJ010000305         3877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_45813, whole genome shotgun sequence.
ACCESSION   DASUGJ010000305 DASUGJ010000000
VERSION     DASUGJ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45813"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFV93_06715"
     CDS             complement(<1..262)
                     /locus_tag="VFV93_06715"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX5165069.1"
                     /translation="MDDPIAGEIRLAAFDQIPAGWASCDGQLLQIADYRALYDVIGTR
                     YGGDGRRTFALPDLRGRLALHADANFPLGATGGRASHALTVNE"
     gene            complement(296..>3877)
                     /locus_tag="VFV93_06720"
     CDS             complement(296..>3877)
                     /locus_tag="VFV93_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165070.1"
                     /translation="LRSLAGVTPPDEKYYGDLALNDIFASLRLLWEASVVNAPGFFLE
                     YDGTEGLPDDAFAGTETADLSLVIVFSSASGMPSYANGLVYSGRPASEERIYVESLDD
                     AVKTAVPTAVPGAVAFYAGRDVVADANASDSVRQTATLFNLVAFRIKDAGSHAGNWAV
                     SGESWSMPYGPLKLDEAHLPDSIKNSEWKYEILVPWTKFLTGGRTQSPLFATTGAQSP
                     YDLAGGDVQLEIAALDTFGNQFPRTVDATFNVRYTDPLVSIPQWPELKSAYHVNTDGQ
                     IVLILEFSADRLSQDSDATRARARALRAQYETIWFQLKDPNTTITLHTTLKSGNGEIG
                     LADGTTINATLLGWVEGVLTYLNAKVGGTAATAPAMKVITTSAIDRATRDAQTENIHP
                     LRVSVQMSRPESMVDADAAARIQAIGSITSDFKADTELLPDLPDLGDGLTGLQSDSMV
                     GFAQKFEAAFPELRVATGDGEGNDQSLWTIRMGHGLGDSGPGIRVNVQPTQRSYFGTA
                     PLSTQFESCVITDPDDASATVQFDNVDMDKLGRRFVALIDDMLSEAKVVKLRRADATN
                     YVKVLDAKQTLAAGVPNGMLSIFTESDNEDRRTASREVFRQSLLKSLSASYDVDTVIS
                     YDVTVSNSEAVSGTPPNLFGRIISLDPVDENEKEQAFVVGTAKVRLGNDANGNAPKLS
                     FTFSTDRDSERAQLPARLAYQITYVEHDIQTDKPAWAPPSGTYEYRGSNWLKLILPDN
                     PRLLNDSRLPNDPIQLSPDGPTNVPIPLREIPTTPVVLRQLGTPEVAAGDSVTLDKAA
                     AWEYEYQIERPRAAQDRIYLHIAFNKKQGNLSLLADEETLLHALCRFDLMYGRLIGAD
                     KPVAEQTQPEPSELLSLLPYIEDVAAKWGSWVPSVPLVQYTWVKDDWAYYMSETVNSS
                     DPDHPSILITFKPLLIKEPVAGKSYGDFPEIRVPVPLGNGSQELTHMPAGAAPGDDVS
                     YSFIWSGNVPDNQTRSFVFSDLNVLKTENARGRMWLTRNENLGEQDGLTTNERFIYRS
                     GASEFTEPVQPFIDRTVPVDIIASVPAPSSQTLAGYLEALFTEIFSIAIDNGYPWPLT
                     KINCRYGYDPRNAVSSPTGPDPNGQFVVYMPVLEHVPFTPTSASAFANDIQSAIQTWE
                     QHVDIPNSPTRGTGFYEFDLSVFSTLNRTNTSMPMLRLRRLWIKRLG"
BASE COUNT          606 a         1230 c         1317 g          724 t
ORIGIN      
        1 gctcgttgac cgtcagcgcg tggcttgccc ttccgcccgt cgcgccgagc ggaaagttgg
       61 cgtccgcgtg gagcgctagc cggccgcgca ggtcaggcag cgcgaacgtc cgccggccat
      121 cgccgccgta gcgggtgccg atcacgtcgt agagcgcccg gtagtcggcg atttgcagca
      181 actggccgtc gcaactcgcc cagccggctg gtatctgatc gaacgcggcg aggcgaatct
      241 cgccggcgat tgggtcatcc attgcggtcc atccgccttt cagcatccgt gcgtgctagc
      301 ccaaacgctt gatccagagt cgtcgcagcc gcagcatcgg catgctcgtg ttggtgcggt
      361 tcagcgtgct gaagacgctg aggtcgaact cgtagaagcc agtgcctctc gtcgggctgt
      421 tcgggatatc gacgtgctgc tcccaggtct ggatcgccga ctggatgtcg tttgcgaacg
      481 cgctcgcgct ggtcggcgtg aacggcacgt gctccagcac cggcatgtag acgacgaact
      541 ggccgttcgg gtccggtccg gtcggcgacg agaccgcgtt gcgcgggtca tagccgtagc
      601 ggcagttgat cttggtcagc ggccacggat agccgttgtc gatcgcgatg ctgaagatct
      661 cggtgaacag cgcttcgaga tagccggcca gggtctgcga cgacggcgcc ggcacgctgg
      721 cgatgatgtc gaccggcact gtccggtcga tgaacggctg gaccggctcg gtgaactcgc
      781 tcgcgccact gcgatagatg aagcgctcgt tggtcgtcaa gccgtcctgc tcgcccaggt
      841 tctcgttgcg ggtcagccac atccggccgc gcgcgttctc cgtcttgagg acgttgaggt
      901 ccgagaagac gaacgagcgc gtctgattgt ccggaacgtt gccgctccag atgaaggagt
      961 agctgacatc atcacccggc gccgcaccgg ccggcatatg ggtcagctcc tgcgagccgt
     1021 tgccgagcgg aaccggaacg cggatctccg ggaagtcgcc gtaggacttc ccggccaccg
     1081 gttccttgat cagcagcggc ttgaacgtga tcaggatcga cgggtgatcg ggatccgacg
     1141 agttgaccgt ctcggacatg tagtaggccc agtcgtcctt gacccaggtg tattgcacga
     1201 gtgggacgga cggcacccag ctgccccact tggccgcgac atcctcgatg tacggcagca
     1261 ggctgagcag ctcggacggt tccggctggg tctgctcggc gaccggcttg tcggcgccga
     1321 tcagccggcc atacatcaga tcgaaccggc agagtgcgtg caacagcgtc tcttcgtcgg
     1381 ccagcagcga gaggttcccc tgcttcttat tgaatgcgat gtgcaggtag atgcggtcct
     1441 gggcggcacg tggccgctcg atctgatact cgtactccca ggcagccgcc ttgtcgagcg
     1501 tcaccgaatc accggcggcc acctccggcg tcccgagctg gcgcaggaca accggcgtcg
     1561 tcgggatctc gcgcagcgga atcgggacgt tggtcggccc atccggcgag agctggatcg
     1621 ggtcgttcgg cagtctcgaa tcgttgagca gtcgcgggtt atccggcagg atcagcttga
     1681 gccagttcga gccgcgatac tcgtaggttc cgctcggcgg cgcccaggcc ggcttgtcgg
     1741 tctggatgtc gtgctcgacg taggtgatct gataggccag ccgggccggc agttgcgccc
     1801 gctcggagtc ccggtcggtc gagaaggtga aggagagctt cggcgcgttg ccgttggcgt
     1861 cgttgcccag ccggaccttg gccgtcccaa ccacgaacgc ctgttccttc tcgttctcat
     1921 cgaccggatc gagcgagatg atccggccga acaggttcgg tggcgtcccg ctgaccgcct
     1981 ccgagttgga gaccgtcacg tcgtagctga tgacggtgtc gacgtcgtac gacgccgaga
     2041 gcgacttcag cagcgattgg cggaagacct cgcgcgaggc ggttcgccga tcttcgttgt
     2101 cgctttcagt gaagatcgaa agcatcccgt tgggcacgcc ggctgccagc gtctgcttgg
     2161 cgtccagcac cttgacgtag ttcgttgcgt cggcacgccg cagcttgacg accttcgcct
     2221 ccgagagcat gtcgtcgatg agggcgacga agcgccggcc gagcttgtcc atgtcgacgt
     2281 tgtcgaactg gaccgtcgcc gaggcgtcgt ccgggtcggt gatgacgcag ctttcgaact
     2341 gggtcgagag cggtgccgtg ccgaagtacg accgctgcgt tggctggacg ttgacgcgga
     2401 tgcccggccc ggaatcgccc aggccgtgtc ccatccggat cgtccagagc gactggtcgt
     2461 tgccctcgcc gtcaccggtg gcaacccgca gctcggggaa ggctgcttcg aacttctggg
     2521 cgaaaccgac catcgagtcg gactgcagtc cggtcaagcc gtcgcccagg tccggcagat
     2581 cgggcaaaag ttccgtgtcg gccttgaagt ccgatgtaat tgagccgatc gcctggatcc
     2641 gcgcggcggc gtcggcgtcg accatgctct ccggccgcga catctggacc gagacccgca
     2701 gcgggtggat gttctccgtc tgcgcgtcgc gggtggcgcg gtcgatcgcg ctggtcgtga
     2761 tgaccttcat ggccggcgcc gtcgcggctg tgccgccaac cttggcgttc aggtaggtca
     2821 ggacgccctc gacccagccc agcagggtcg cgttgatcgt cgtgccatcc gccaacccga
     2881 tttcgccgtt gccggacttg agcgtcgtgt ggagcgtgat cgtcgtgttc gggtctttga
     2941 gctggaacca gatcgtctcg tactgcgccc gcaacgcgcg tgcacgggcg cgtgtggcgt
     3001 ccgaatcctg gctgagccgg tcggcgctga actccaggat caggacgatc tggccgtccg
     3061 tgttgacatg gtaggcggac ttgagctccg gccactgtgg gatcgagacc agcgggtcgg
     3121 tgtagcgtac gttgaacgtc gcgtcgaccg tccgtgggaa ctggttgccg aacgtgtcca
     3181 gcgccgcgat ttcgagctgc acatcgccac cggccaggtc gtacggactc tgcgcaccag
     3241 tcgtcgcgaa cagcggcgat tgtgtccggc cgccggtcag gaacttcgtc cacgggacga
     3301 ggatctcgta cttccactca gagttcttga tcgaatccgg caggtgcgcc tcgtccagct
     3361 tgagcgggcc gtacggcatc gaccagctct cgccgctgac cgcccagttg cccgcgtgcg
     3421 agccggcatc cttgatccgg aaggcgacca ggttgaacag cgtcgccgtc tggcggaccg
     3481 aatcggacgc gttggcgtcg gctacgacat cgcggccggc gtagaaggcc accgcgccgg
     3541 gaacggccgt cggaacggcc gtcttgaccg cgtcgtcgag gctctcgacg tagatgcgct
     3601 cctcggacgc cggccggccg gagtaaacca ggccgttggc gtacgacggc atgccgctcg
     3661 cgctcgagaa gacgatgacc agcgacagat cggccgtctc ggtgccggcg aatgcgtcgt
     3721 ccggcagtcc ttccgtgccg tcgtattcca ggaagaagcc cggcgcgttg acgacactgg
     3781 cctcccagag cagccgcagg ctggcgaaga tatcgttgag cgccagatcg ccgtagtact
     3841 tctcgtccgg cggcgtcaca cccgccaggc tgcgcag
//
LOCUS       DASUGJ010000306         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1434424, whole genome shotgun sequence.
ACCESSION   DASUGJ010000306 DASUGJ010000000
VERSION     DASUGJ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434424"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="VFV93_06725"
     CDS             complement(<1..1359)
                     /locus_tag="VFV93_06725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5165071.1"
                     /translation="MTVESTTGALPGRVAGEQRFTVERDQLPAVAAAVVEELDARLAL
                     MVGEDDRAASGAYRLSYVFAPRTGGWVTLETAVYPIDPSFPSVTPLVPAAHWYEREVR
                     DMFGLEPIGHPDPRRLVLHDDWPEDVHPLRKDFDPSEPVPRETVHRHEFHRLHGEGFV
                     EIPVGPIHAGVIEPGHFRFAAVGEAIFHLETRLFYTHRGIEKLAEGKTPQHLLQIAER
                     ICGACSLSHGVAYSQAVEAIAEVELPERAAALRTIYLELERLYNHIGDLGNICAGVGF
                     AVGTTYGARLKEELQRLNERLTGNRFLHGVNRIGGVRCDLTAAGVSDIQATLQQTAID
                     FQRFVDLLLDSDTLIERLTGTGVLPATTARALGAVGVAARASGIDTDSRRDHPHAYYD
                     RLPQGHVPVVVRHEGDVLARALVRVDETLASLAIVRGLLDNLPAGPAFVPVGDIPGGR
                     AAF"
     gene            complement(1377..>2213)
                     /locus_tag="VFV93_06730"
     CDS             complement(1377..>2213)
                     /locus_tag="VFV93_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5165072.1"
                     /translation="APLHTWLADAHSQAPSPVSGVLSGVLLACALYAILRFHTLTTAA
                     TASNFSGNLLLAFGVVSVAVAAPFVLVQRDLKRLLAYSSVEHIGLMAVAFGIGGALGV
                     YAGVLHLVNHAATKSLLFFSAGDVVQRFGTRRISAIHGALRVAPLSGWLLLLGVFAIT
                     GGPPSGIFISEVAIAGAGFGGDRWRVAASVVVVLLLGAIFAGILAQTLRLAYGTPPAA
                     LVSGGGTGHRWWSDGPRLAAVAPLVAIVVLFGIYVPGPVSDLLHDVATMVAPVSGTAA
                     RP"
BASE COUNT          381 a          777 c          765 g          290 t
ORIGIN      
        1 gaaggcggcc cggccaccgg ggatgtcgcc gaccggcacg aacgccggcc cggccggaag
       61 gttgtcgagc aatccgcgca cgatcgccag gctggccagc gtctcatcga cgcgaaccag
      121 tgcccgggcc aggacatccc cctcgtgtct gacgacaacc ggcacatgtc cctgggggag
      181 ccggtcgtag taggcgtgcg ggtggtcgcg gcggctatcg gtgtcgatgc cgctggcccg
      241 cgcggcgaca ccgaccgcgc cgagcgcccg agccgtcgtg gccggcagga cacccgtccc
      301 ggtcaggcgc tcgatcagcg tgtcgctgtc gagcagcagg tcgacgaagc gctggaaatc
      361 gatggcggtc tgctgcaacg tcgcctgaat gtcagacacg ccggccgccg tcagatcgca
      421 gcggacgccg ccgatccggt tgacgccgtg caggaagcgg ttgccggtca gacgctcatt
      481 gaggcgctgt agctcctcct tgagccgcgc gccataggtc gttccgaccg cgaagccgac
      541 accggcgcag atgttgccga ggtcgccgat gtggttgtag agccgctcca gctccaggta
      601 gatcgtccgc agggccgccg cccgctccgg cagctcaacc tcagcaatcg cctcgaccgc
      661 ctgcgaatac gccacgccat gcgacagcga gcaggcgcca cagatgcgct cggcgatctg
      721 caacaggtgc tgcggcgtct tgccctccgc cagcttctcg atgccccggt gggtgtagaa
      781 gagtcgggtc tccaggtgga agatcgcttc gccgacggcc gcgaagcgaa agtggcccgg
      841 ctcgatcacg ccggcgtgga tcggcccgac cggaatctcg acgaatcctt cgccgtgcaa
      901 gcggtggaac tcatggcggt ggaccgtctc acgcggaacc ggctcactcg ggtcgaagtc
      961 cttgcgcagc gggtggacat cttcaggcca gtcgtcgtgc agcacgagcc ggcgcgggtc
     1021 cgggtggccg atcggctcga gcccgaacat gtcgcgcacc tcacgctcgt accagtgggc
     1081 ggctggaacg agcggcgtca ccgacgggaa cgacgggtca atcgggtaga cggccgtctc
     1141 aagcgtcacc cagccgccgg ttcgcggcgc gaagacatag gagagccggt acgcgcctga
     1201 cgctgcccgg tcatcttcgc caaccatcag cgccagccgc gcgtccagct cctcgacgac
     1261 ggccgcagcg acggccggga gctggtcccg ctcgaccgtg aagcgctgct ctcccgccac
     1321 acgtccgggc aacgcgccgg tcgtgctctc aaccgtcatc tgcacgtctg cctcattcat
     1381 ggtcgcgccg ccgttccgga cacgggcgcg accatcgtcg ccacatcgtg cagcaggtcg
     1441 gataccggcc ctggtacgta gattccgaac aacacgacaa tcgccacgag cggcgcgact
     1501 gccgccagcc gggggccgtc cgaccaccag cggtgacccg ttccgccacc actcacgagc
     1561 gcagccggtg gcgttccata cgcgagccgc agcgtctggg ccaggatgcc ggcgaagatg
     1621 gcgcccagca gcaggacgac gacgacgctg gcggcgaccc gccagcggtc gccgccgaag
     1681 ccggccccgg cgatcgccac ctcggagatg aagatccccg acggtggccc gccggtaatg
     1741 gcgaagacgc cgagcagcag gagccagccg gacagcggcg cgacgcgcaa cgcgccgtgg
     1801 atcgcgctga tccgccgcgt tccgaagcgc tggacgacat cgccggcgga gaagaacagc
     1861 agcgacttgg tcgccgcgtg gttgacgagg tgcaggacgc ccgcgtagac ccccagcgcg
     1921 ccaccgatcc cgaaggcgac cgccatcagc ccgatgtgct cgacgctgga atacgccagc
     1981 agccgtttca ggtcgcgctg gacgagcacg aacggcgctg cgaccgccac cgagacgacg
     2041 ccgaacgcca gcagcaggtt gccggagaag ttgctggcgg tggccgccgt cgtcagggtg
     2101 tgaaagcgca ggatcgcgta gagcgcgcag gccagcagca ctccggacag gacgcccgag
     2161 accggcgagg gagcttgcga gtgggcgtcg gccaaccagg tgtgcaaggg cgc
//
LOCUS       DASUGJ010000307         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_463641, whole genome shotgun sequence.
ACCESSION   DASUGJ010000307 DASUGJ010000000
VERSION     DASUGJ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463641"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /gene="purM"
                     /locus_tag="VFV93_06735"
     CDS             complement(<1..1035)
                     /gene="purM"
                     /locus_tag="VFV93_06735"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5165073.1"
                     /translation="MLSYRGAGVDIDAADAVVAGIRQLAARTHGDRVPKGLGHFGGFY
                     RIGPTGAGMTLVASIDGVGTKLKIATLAGQHDGIGADIVNHCLNDIAACGATPLFFLD
                     YFGTGRLNPERAVTVIGGIARACEEAGVALIGGETAEMPGVYSGEDYDLVGTIVGMVD
                     AGAIVDGSAVEIGHVLVGLPSRGFHTNGYSLVRAALGLNESEAARERLAGPAPFDSAR
                     SLASALLEPHHSYLPVVRNLLAGAHVAGMAHITGGGLPGNVSRIIPEGATARIETSRW
                     RIPELFSFVAEAGNVSVAECFRAFNMGIGYVVICPPDDVPNVTSIAQDAVVIGEVVAA
                     GDERRVVLTQD"
     gene            complement(1112..1477)
                     /locus_tag="VFV93_06740"
     CDS             complement(1112..1477)
                     /locus_tag="VFV93_06740"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX5165074.1"
                     /translation="MAASLADVLQELQSSSGLEMAAVVSSDGLVIDSASSGEVDVESL
                     CSVASNGVLVMDALGQELGENASEMMTVEYGRHIVVMSQVDPENLLVLLGGPGINLGR
                     MRIILRRRMDALTEALENE"
     gene            complement(1600..2943)
                     /gene="hflX"
                     /locus_tag="VFV93_06745"
     CDS             complement(1600..2943)
                     /gene="hflX"
                     /locus_tag="VFV93_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX5165075.1"
                     /translation="MERSDPINERGPYPTQSPQQRAFLVSVDYRRDDWTADASLEELA
                     RLSETAGLDVVGSATQKLEHPHPKTYVGKGKLEEIVDAVAEIRADVVIFDDELTPAQL
                     RNVEDRLETMVIDRTALILDIFAKRAVTHEGRLQVELAQLEYRLPRLTRMWTHLERQG
                     VGGVGLRGPGETQLETDRRLARARISHIKSELADVHRHRELYRTRRRRNNVPVVAIVG
                     YTNAGKSTLLNRLTDAGVLSEDQLFATLDPTTRRITLPGGRDVLLTDTVGFINHLPTT
                     LIAAFRATLEEIEEASVLLHVLDISHPNAPEQAETVREVLAELDVVGKPMVTALNKID
                     MLDEDADPAAVAFDLNLPDDFVPISAMQNLGIDTLLLRIEEALGLAAGVTRIDVCIPY
                     DRAELVNLFHRAGKVESVTHGDDGTHLTGTLPRRLRERFEPFVQAGQTVVAADGS"
     gene            complement(2999..>3296)
                     /locus_tag="VFV93_06750"
     CDS             complement(2999..>3296)
                     /locus_tag="VFV93_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165076.1"
                     /translation="EVIDGIALKLRGVEVGIEPETLAKVLSPKRFIERRDHPGGPAPS
                     AVRASLERETFASNRARGWVNATREALAAATTVLNTRASEIARDPETALRRLKG"
BASE COUNT          561 a         1136 c         1016 g          583 t
ORIGIN      
        1 gtcctgggtc agtacgacgc gccgctcgtc gccggccgcg acaacctcgc caatcacgac
       61 cgcgtcctgc gcgatgctgg tgacattcgg cacatcgtcc ggcggacaga tcacgacgta
      121 tccgattccc atgttgaatg ctcgaaagca ctcggctact gacacgttgc ccgcctcggc
      181 aacaaacgag aacagttccg gtatccgcca tcggctcgtc tcaatccgcg ccgttgcccc
      241 ttcggggatg atgcgcgaga cgttgcccgg caggccgcca cccgtgatat gcgccatccc
      301 ggccacgtgt gcgccggcta gcagattccg aacgaccggc agatacgaat gatgcggctc
      361 cagcaaggca ctcgccaggc tacgggctga atcgaacggc gctggcccgg ccagtcgctc
      421 ccgtgccgcc tcactctcat taagccctaa agcggcccgg acgagggagt acccattggt
      481 atgaaatcca cggctgggaa ggccgactaa cacatgaccg atctctacgg ccgacccgtc
      541 gacaatcgcc ccggcgtcta ccatgccgac gatcgtcccg acgagatcgt agtcctcacc
      601 gctgtaaacg cccggcatct cggccgtctc gccgccgatc agcgcgacgc cggcctcctc
      661 gcaggcgcgg gcgatgccac cgataacggt cacggccctc tcggggttga gccggccggt
      721 cccgaaatag tcgagaaaaa acaagggtgt cgcgccgcag gcagcaatgt cgttgaggca
      781 gtggttcacg atgtcggcgc cgatgccatc gtgctgaccg gccagcgtgg cgatcttcag
      841 cttcgtcccg acgccgtcga tgctggcgac cagcgtcatg cccgcgcccg ttggaccgat
      901 ccggtaaaag ccgccgaagt gcccgagccc cttcggaacg cggtcgccat gagtccgagc
      961 cgccagctgg cgaatcccgg cgacgaccgc gtcggcggcg tcgatgtcga cccctgcccc
     1021 gcggtagctc agcatcccgt cggcttgatc atcaggctgc tggatgttcg cccccactcg
     1081 tctgacgcga ccgaccgacc ggcccaccgg cttattcgtt ctccagcgcc tcggtcagtg
     1141 cgtccatccg ccggcgcagg atgatgcgca tgcgcccgag gttgatgccc ggcccgccca
     1201 gcagaacgag caggttctcc ggatcgacct gtgacatcac gacgatatgc cgaccgtact
     1261 cgaccgtcat catctccgag gcgttctcgc caagctcctg cccgagcgcg tccatcacga
     1321 gcacgccgtt ggatgccacc gaacacagcg actcgacatc gacttcgccc gatgacgcag
     1381 agtcgatgac cagcccatcg gaactgacga ccgcggccat ctctagtcca ctcgaagact
     1441 gcaattcctg aagcacgtct gccagactgg ccgccatccg tcgtccccct cttcgcccta
     1501 gcgtcatctc gacctcggga ttatagccgc gcaggagtcg tacacgcggc ccgcaatttg
     1561 gacgcgggtg gattcatact tttgtactac gggaggttct cagctgccgt cagcggcaac
     1621 caccgtctgg cccgcctgga cgaacggctc gaatcgctcg cgcagccgac gcggcagcgt
     1681 tcccgtcaga tgggtcccgt catcgccgtg cgtgacggac tcgaccttgc cggcgcggtg
     1741 gaacaagttg accagttcgg cgcggtcgta agggatacag acatcgatcc gggtaactcc
     1801 ggctgccagc ccgagcgcct cctcgatccg tagcagcaac gtgtcgatcc ctagattctg
     1861 catcgcggag atcggcacga agtcgtccgg caggttcagg tcgaacgcga cagcggccgg
     1921 gtcggcatcc tcgtcgagca tgtcgatctt gttgagggct gtcaccatcg gcttgccgac
     1981 gacatccagc tccgccagca cctcgcggac cgtctcggcc tgctccggcg cgttcggatg
     2041 cgagatatcg aggacgtgca gcaggaccga cgcctcctcg atctcctcaa gtgtcgcccg
     2101 aaaggccgcg atcagtgtcg tcggcaggtg attgatgaag ccgaccgtgt cagtcagtaa
     2161 cacgtcgcgt ccacccggca gcgtgatccg ccgcgtcgtc gggtcaagcg tcgcgaagag
     2221 ctggtcctcc gacaggacgc ccgcatcggt cagccggttg agcagggtcg acttgccggc
     2281 gttggtgtag ccgacgatcg cgacgaccgg cacgttgttg cgccggcggc gtgtccgata
     2341 cagctcgcga tgccggtgga cgtcggccag ttcggacttg atgtgcgaga tgcgcgcccg
     2401 cgccagccgc cggtccgttt cgagctgtgt ctcaccaggg ccgcgcaggc cgacgccacc
     2461 gacgccctgc cgctccaggt gtgtccacat ccgcgtcagc cggggcagcc gatattcgag
     2521 ctgcgccaat tcgacctgca agcggccctc atgagtcacg gcccgcttgg cgaagatgtc
     2581 cagaatgagc gccgtccggt cgatgaccat cgtctccagc cggtcttcga cgttgcgtag
     2641 ctgggccggg gttaactcgt cgtcgaagat cacgacatcg gcacggatct ccgcgacggc
     2701 gtcgacgatc tcttccagct tccccttgcc gacgtaagtc ttggggtgcg gatgctccag
     2761 cttctgggtc gcagagccaa cgacatcaag cccggccgtc tcagagaggc gggcaagctc
     2821 ttcaagcgac gcgtcggcgg tccagtcgtc gcgccggtaa tcgacgctga cgaggaaggc
     2881 gcgttgctgt ggggactgtg ttgggtatgg tccgcgttcg ttgatggggt cgctccgttc
     2941 aataggatgc ggaatgatcc ggaaatggta ccattcggcg ctcccatcac tcgcacgctc
     3001 agcccttcaa tcggcgcagc gcggtctccg gatcgcgggc gatctcggag gcgcgtgtgt
     3061 taagaaccgt cgtcgccgca gcgagtgcct cgcgtgtggc gttcacccag cctcgtgcgc
     3121 gattggacgc gaacgtctcg cgctccagtg atgcccggac ggcgctcggc gccggcccgc
     3181 ccggatgatc ccgccgctct atgaagcgct tcggcgacag aaccttcgcg agcgtctccg
     3241 gctcgatgcc gacctcgacg ccgcgcagct tgagcgcgat cccgtcgatg acttcg
//
LOCUS       DASUGJ010000308         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_880877, whole genome shotgun sequence.
ACCESSION   DASUGJ010000308 DASUGJ010000000
VERSION     DASUGJ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880877"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VFV93_06755"
     CDS             complement(<1..857)
                     /locus_tag="VFV93_06755"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEX5165077.1"
                     /translation="MPLLITPSYDAKTWGGRRLEQLGKILPGGAIGESLESGPAAVVN
                     GGPFDGVTLGELARTHAAALLGKRGQAAAAAFADFPLLVKLIDAHQDLSVQVHPDDAR
                     APAGKRGKTEAWYILDAEPGARIITGVTGLIQVDRIGEQLVETVVRPGEVYFVPAGTV
                     HAIGAGVLLYEIQQASDVTWRLFDWGRPREIHVEAALAAADPDRQATRIESCRITQDR
                     EMLVACRYFALERWTIDDRTALPAFPLAFRVVTVLEGELVAGNVRVPHGATVVLPADL
                     PESELTGRG"
     gene            complement(881..2068)
                     /locus_tag="VFV93_06760"
     CDS             complement(881..2068)
                     /locus_tag="VFV93_06760"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5165078.1"
                     /translation="MRESDVAVVGAGPAGIGAALAAARNGVSVVLIDENSAVGGHLRW
                     TLAPQTGFEPALDGLTGIEIAGWAAGALADAGVGVLTDSVAWGLFEENVLGVATPDAS
                     FRLRARAVVVATGSTDSVWPFPGWQLPGVMTATAALRAMHLDRVLPGFRCVVLGAGSL
                     AEVVASDLTSCGAAVVLRAGNVDGLVAGGDGAVAWVESDGQRTACDAVILAFGRQPDA
                     ELALQAQAALAYSPNDGVFVPWRDETLATRLAGVYVAGDAGGVGTAARSYVEGLVAGA
                     AASGGDGLTDALERLREIAADQVPDSPLPAIADATLVCRCEEVSAASIRTAINTGAIS
                     LNDIKRRTRAGMGICQGIYCHRTVAQMLSTETGIPLDTIVPMTARPPARLLSMAAMAD
                     LET"
     gene            complement(2068..2406)
                     /locus_tag="VFV93_06765"
     CDS             complement(2068..2406)
                     /locus_tag="VFV93_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5165079.1"
                     /translation="MAQPATSRRIRVHPVLGEDLRRDVVAITVDGQPLPAFAGEPIAA
                     ALLAHGITAGRTMPETDAPRGYFCGVGRCPDCSMTVGGELNVRTCVTPVRDGMVIETQ
                     RGLGIWKVGG"
BASE COUNT          432 a          892 c          801 g          368 t
ORIGIN      
        1 ataccccgtc cggtcagttc gctctcggga agatctgccg gcagaacgac ggttgctccg
       61 tgtggcacgc gaacgttgcc ggcgaccagt tccccctcca gcacggtgac gactctgaac
      121 gccagcggaa aggccggcaa ggcggtgcgg tcatcgatcg tccagcgctc aagcgcgaag
      181 taccgacaag cgaccagcat ctcacgatcc tgagtgatgc ggcacgactc gatacgcgtg
      241 gcctgccggt cgggatcggc agctgcgagc gcggcttcga cgtggatctc gcgcggtcgg
      301 ccccagtcga acaaccgcca tgtgacatca ctcgcctgct gaatctcgta gagcagcacc
      361 ccggcgccaa tcgcgtggac ggtaccagcc ggcacgaaat agacctcgcc gggtcgaacg
      421 accgtctcga cgagttgctc gccgatgcga tcgacctgga tcagcccggt cacgccagtg
      481 atgatccgcg cgcccggctc ggcgtcgagg atgtaccagg cttcggtctt gccccgcttg
      541 ccggccgggg cgcgggcgtc gtccgggtga acctggacgg agaggtcctg atgggcatcg
      601 atcagcttga ccagcaacgg gaagtcagca aacgcagcgg cggccgcctg gccacgcttg
      661 ccgagcagcg cggcggcatg tgttcgcgcc agctcgccga gggtcacgcc atcgaacgga
      721 ccgccgttca cgacggcagc cgggccgctt tcgagcgatt caccgatcgc gccgcccggc
      781 aggatcttgc cgagttgctc cagccgccgc ccgccccagg tcttcgcgtc gtagctgggc
      841 gtaatgagca atggcacggc gatcctgccg gaactggcgt tcatgtttcc agatcggcca
      901 ttgccgccat cgagagcagc cgggccggag gacgcgccgt catcggcacg atcgtgtcca
      961 gcggaatacc ggtctccgtc gagagcatct gggcgacggt ccggtggcaa tagatcccct
     1021 ggcagatgcc cattccggca cgcgttcgac gcttgatgtc gttcaggctg atcgcgccag
     1081 tgttgatcgc cgttcggatc gatgcagcgc tgacttcctc gcagcggcag accagcgtcg
     1141 catcggcgat ggccggcagc ggtgaatccg gcacctggtc ggctgcgatc tcgcgaagcc
     1201 gttcgagcgc gtcagtcaga ccatcgcctc ctgacgcagc cgcccccgca accagtcctt
     1261 cgacatacga ccgagccgcc gtacctactc cgcctgcgtc gccggcaacg tagacaccgg
     1321 cgagccgggt ggcaagcgtc tcgtcgcgcc atggaacgaa gacgccatcg ttcggcgagt
     1381 atgcgagcgc cgcctgcgcc tggagcgcca gttccgcgtc cggctggcgg ccgaaggcaa
     1441 ggatcaccgc gtcgcaggca gtgcgttgcc cgtccgactc gacccatgcg acggcgccgt
     1501 ccccgccggc aaccagaccg tcgacgttgc cggctcgcag gacgacagca gcgccacaac
     1561 tcgtcagatc ggacgcgacg acctcggcaa gcgacccagc gccgagcaca acgcagcgga
     1621 agcccggcag cacccgatcg aggtgcatcg ctcgaagtgc ggccgttgcc gtcatcacac
     1681 cgggtagttg ccagccggga aacggccaga cgctgtcggt cgagccggtc gcgacgacga
     1741 cggcacgcgc ccgcaaccgg aacgacgcgt ccggtgttgc gacgccgagg acgttctcct
     1801 cgaagagccc ccaggcgaca gagtccgtca gcaccccgac accggcatcc gccagcgccc
     1861 cagcggccca gccggcgatc tcgatcccag tcagaccatc gagcgcgggt tcgaagccgg
     1921 tctggggcgc caacgtccag cgaagatggc cgccaacggc gctgttctcg tcgatcagga
     1981 cgacgctcac gccgttgcgg gcggccgcca gcgccgcgcc gatcccggcc ggaccagccc
     2041 cgacgacggc gacatccgac tcgcgcatca gccgcccacc ttccagatgc caagtccgcg
     2101 ctgtgtctcg attaccatcc cgtcgcgcac tggcgtgaca caggttcgga cattcagctc
     2161 accgccaacg gtcatcgagc agtccgggca acggccaacg ccgcagaaat agccgcgcgg
     2221 cgcgtccgtt tcaggcatcg ttcggccggc cgtgatgccg tgtgccagca gcgccgccgc
     2281 gatcggttcg ccggcaaagg caggcagagg ctgaccatcg accgtgatcg cgacaacatc
     2341 gcgtcgcaga tcctcaccga ggaccggatg gacacggatg cgtctcgacg ttgctggctg
     2401 cgccatgatt cccctctccc gcgacccgcc gaccggctgg gatgatcttt ccagccgctg
     2461 gccgagccgt caaccgtcca gagcagccgt tgt
//
LOCUS       DASUGJ010000309         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1434604, whole genome shotgun sequence.
ACCESSION   DASUGJ010000309 DASUGJ010000000
VERSION     DASUGJ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434604"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..559
                     /locus_tag="VFV93_06770"
     CDS             152..559
                     /locus_tag="VFV93_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5165080.1"
                     /translation="MQARLQSVPVSVNAPLVGRIVSGMAALFLAIDGVVKLATPEPVV
                     RAMDELGYPDRMAVGIGALLLACLAVYLYPRTSALGAVLLTGYLGGAIASQVRIEADL
                     FPVVFPAIVGTLIWTGLYLRDARLRALLAPRGE"
     gene            617..1096
                     /locus_tag="VFV93_06775"
     CDS             617..1096
                     /locus_tag="VFV93_06775"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5165081.1"
                     /translation="MRYLCLVYQDERQLAAMTSSDYDALVDEVRAYIEEATLSGHYLA
                     SNALEWVESATSIRVRDDRLSITDGPFVETKEQLGGYLLIEARDLNEAIRVAARMPSA
                     RFGGIEIRPIKEVAAGRAAKQAGPVDAPSPSLERPGQGDAQVVASEPASEWTQVRVS"
     gene            1093..1662
                     /locus_tag="VFV93_06780"
     CDS             1093..1662
                     /locus_tag="VFV93_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5165082.1"
                     /translation="MRHVVYSLNMSLDGFMARPDGDLDWVIVTEELHAFVNERERNID
                     THLYGRRMYETMAAYWPTADRNPDAHPTEVEYALIWQGIEKVVFSTTLDRVEGNSRLV
                     RDDIAGEVAALKAQPGKNIGVGGATLAASLLQLGLIDTYEIYLQPVILGEGIPMFPSP
                     AATSSLRLVDSRTFDSGVVFLHYERSGEE"
     gene            1667..1861
                     /locus_tag="VFV93_06785"
     CDS             1667..1861
                     /locus_tag="VFV93_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEX5165083.1"
                     /translation="METNDLPKIPNPARRALANAGIDRLDQLTTRREAELLTLHGMGP
                     KAIAELHEALAARGLAFADD"
     gene            1959..3239
                     /locus_tag="VFV93_06790"
     CDS             1959..3239
                     /locus_tag="VFV93_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165084.1"
                     /translation="MFAILRNRDFGLLWLAGLISLAGTRAFIIALPIHVYRLTDSTLA
                     TAGAFAASFLPGILFGSLAGVFVDRWDRKTTMVVSNVVQALLLLPILAAPDSLTLLYT
                     IAAVQGTVGLFFSPAESAFLPTLVGKDKLVAANAMNSLNDNLALLIGPATGAMLYAQI
                     GFAGAAMFDAASYAIAAVLIRLIAASGKPERMPETPTTAPVRTMLDDWKGGMRVIRND
                     TSLKVLFAAFSFNGVAEGVFLSLGLAPLVLDVLGGTAAQVGWLATIQAVGGIVAGIII
                     VRIGHRFSLRWLIGGGMIGLGLADFCFFNSYRLASPGNASVAVAMVFMALAGLPAVAG
                     ASGRQTLVQEQTTDAFRGRVFGALRSAQGLTMLVGLAIGGVFGDSIGLVPVLSASAIV
                     RMLGGVLAFALLPSPSAARQHLATSPQAASAKPD"
BASE COUNT          514 a         1114 c         1043 g          578 t
ORIGIN      
        1 cgattttgcg aaatcgcccc taccggattg ggggatttaa gaatgaacca ccttcagccc
       61 aattgtcgat ttcgcccctc cccaaacgac tacttgatgt catgggtatt caccgtgacc
      121 gtcgaacaca tgaaagagag aggtgccagt catgcaggca cggttgcagt cggttccggt
      181 atcagtcaat gcaccgctgg ttgggcggat cgtgagcggg atggcggcct tgtttctggc
      241 tatcgacggc gtggtgaagc tggcaacgcc ggagccggtc gtcagggcga tggacgagct
      301 tggctatccg gaccggatgg cggtcgggat tggcgcgctg ctgctggcct gcctggccgt
      361 ctacctctac ccgcgaacct ccgccctcgg cgctgtcctg ctgaccggct atctcggcgg
      421 cgcgatcgcg agtcaggtcc gaatcgaggc cgatctcttc ccggtcgtct tccccgccat
      481 cgtcggcacg ctgatctgga ccggcctcta cctgcgcgac gcccggctgc gggcgctgct
      541 cgccccgcgc ggcgagtgat cgtcgcaaca gggtgcgccg gcggggccac cgcgagtgac
      601 caggttggag ggagccatga gatatctatg ccttgtctat caggatgagc gacagttggc
      661 cgccatgaca tcgagcgact acgacgcgct cgtcgatgag gtacgcgcct acatcgagga
      721 ggcgacgctg agcggccact acctcgcctc gaacgcgctg gagtgggtcg aatcggccac
      781 ctcgatccgc gtccgcgacg acagactctc gatcaccgac ggcccgttcg tcgagacgaa
      841 ggagcagctc ggcggctacc tgctgatcga ggccagggat ctcaacgaag cgatccgcgt
      901 cgccgccagg atgccgtccg cgcgattcgg cgggatcgag atccgcccca tcaaggaggt
      961 cgccgccggg cgagcggcga agcaggccgg gccggtggac gcaccgtcgc catctctcga
     1021 acggccagga cagggcgacg ctcaggtcgt cgccagcgag ccggcaagcg aatggacaca
     1081 ggtgagagtt tcatgagaca cgtggtgtat tcactgaaca tgtcgctgga tggtttcatg
     1141 gcgcgacccg atggcgacct cgactgggtc atcgtcaccg aagagctgca tgcgttcgtc
     1201 aacgagcgcg agcgcaacat cgacacgcac ttgtatggcc ggcgcatgta cgagacgatg
     1261 gctgcctact ggccgacggc ggaccggaac cccgacgcgc accccaccga ggtcgaatac
     1321 gccctcatct ggcaagggat cgagaaagtc gtcttctcaa cgacgctcga ccgggtcgag
     1381 ggcaactcgc ggctggtccg cgacgacatc gccggcgagg tcgccgcgct gaaggcccag
     1441 cccggcaaga acatcggcgt cggcggcgcg acgctcgccg cgagcctgtt gcagcttggg
     1501 ctgatcgaca cgtacgagat ctacctccag ccggtcatcc tcggcgaggg cattccgatg
     1561 ttcccctcgc ccgctgccac cagcagcctc cggctggttg actcgcggac gttcgactcc
     1621 ggcgtcgtct tcctccacta cgagcgatcc ggcgaggaat aacaggatgg agacgaacga
     1681 cctgccgaag atccccaacc ctgcccgccg ggcgctcgcc aacgcgggaa tcgaccggct
     1741 cgaccagctg acgacccgtc gcgaggcgga gctgctcacc ctgcatggga tggggccgaa
     1801 ggcgatcgcc gagctgcacg aggcgctggc agcgcgcggg ctggccttcg ccgacgactg
     1861 acgtgagagc gagagtggtt agcgtccgaa cccggcgatc tggcataatc ggcggccttg
     1921 cctgactgtc ccgccgccat ccagcctgcc ggaggaccat gttcgccatc ctgcgcaacc
     1981 gtgatttcgg cctgctctgg ctggccggcc tgatctcgct ggccggcacc cgcgcgttca
     2041 tcatcgcgct gccgatccac gtctaccggc tgaccgattc gacgcttgcg acggccggcg
     2101 ccttcgcggc cagcttcctg cccggcattc tgttcgggtc gctggcgggt gtcttcgtcg
     2161 atcgctggga tcgcaagacg acgatggtcg tctcgaacgt cgtccaggcg ctgctgctgc
     2221 tgccaatctt ggccgcgccc gacagcctga cgctgctcta tacgatcgcc gccgtccagg
     2281 gaacggtcgg gctgttcttc tcgccggcgg agagcgcctt cctgccgacg ctggtcggca
     2341 aggacaagct ggtcgccgcc aacgcgatga actcgctcaa cgacaacctc gcgctgctga
     2401 tcggcccggc caccggcgcg atgctctacg cccagatcgg cttcgccggc gccgccatgt
     2461 tcgacgccgc ctcctacgcc atcgccgccg tcctgatccg cctgatcgcc gcctccggca
     2521 agcccgagcg aatgccggag acacccacaa ctgcacccgt ccggacgatg ctggacgact
     2581 ggaagggcgg gatgcgggtc atccgcaacg acacgtcgct gaaggtgctc ttcgcggcct
     2641 tctcgttcaa cggcgtcgcc gaaggcgtct tcttgtcgct cggtctcgcg ccgctggtgc
     2701 tggatgtcct cggcgggaca gcggcccagg tcggctggct ggcgacaatc caggcggtcg
     2761 gcgggatcgt tgccggtatc atcatcgtcc ggatcggcca tcgcttctca ttgcgctggc
     2821 tgatcggcgg cgggatgatc ggtctcggtc tggccgattt ctgcttcttc aattcgtacc
     2881 ggctcgcttc ccccggcaac gcctccgtcg ccgtcgcgat ggtcttcatg gccctggctg
     2941 gtcttccggc cgtcgccggc gcgtccggca gacaaacgct cgtccaggaa cagacgaccg
     3001 atgcgttccg cgggcgcgtc ttcggcgcgc tccgttccgc ccagggactc acgatgctgg
     3061 tcggcctcgc catcggcggc gtcttcggcg attcaatcgg cctcgtaccg gtcctcagtg
     3121 ccagcgcgat cgtccggatg ctgggcggcg tccttgcgtt cgcactcctg ccgagtccat
     3181 cggcggcccg ccaacacctc gccactagcc cgcaagccgc ctcggctaaa cctgattaga
     3241 ttccctgtc
//
LOCUS       DASUGJ010000310        16581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_185713, whole genome shotgun sequence.
ACCESSION   DASUGJ010000310 DASUGJ010000000
VERSION     DASUGJ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 16581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16581
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185713"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2624
                     /locus_tag="VFV93_06795"
     CDS             <1..2624
                     /locus_tag="VFV93_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165085.1"
                     /translation="DLQRRVEELQREQTEFTEDAERHSNAAVEVRARLSDIEAQLADH
                     ARMAGAARQRRADLRAAIEAAEARLVTIRRELAGIEGTQTSIAERIATVAADRERVST
                     QTANEREQFDRLQHSVADLDAQIARVAERETAIQASIAEHLASATTAADGLAERRAAL
                     QTIEREVTNLQTRLDALERAHASGEGLYAGVRAILRAIDRSQLLLPGLVGTLAETVEV
                     PAELETAIEVALGGHLQDLIVQTWSDAQEAIAFLKRTNAGRATFQPLDTVRPVRRPAL
                     SASDGDLRGVAADLVTFPEQVAPVVEQLLGRTLVVADLDATRRILKSASGWTIVTVGG
                     EIARPSGSVTGGGRAAESGILARERERRALPATLAARNATRAEVQATIERAVAEVEKR
                     ETARRTLQSELESVSRQLRELRADREQQSRSRDATLAALQQADSRAETLEQREQGLRQ
                     ESATLESRTAELEAEQRGAREERARVEALLAAEPEDVDAAAAGLRVELAADQERLHTA
                     ELSARKARERAESAQRSANERTAEIERLREAETDDAQRLRKFDAQILEFEQLVATGQA
                     ALPPIAQQRDEVVELLATAERTLSREAAGLREAERERDRHSLNLARVQDEQVFLVERI
                     RSDLDLDDPSDLEAIEDDAPVPDEGEINRLRDRLRRMSNVGEDVLEQHDAESQRLTYL
                     SGQLADIDEASDGLRRVLSELNSKMATRFTETFREVAVAFEHTFTRLFGGGSARLVMS
                     ANEDGAAGIDIVAQPPGKRLQNLNALSGGERALTAVALLIAIQRVNPSPFCLLDEVDA
                     ALDESNVVRFRDELRDLAGNTQYVVITHNRGTIEGADTLYGVTMGDDGVSRVLSLRLE
                     EAIQAVEAYESAQPAGT"
     gene            2702..3970
                     /locus_tag="VFV93_06800"
     CDS             2702..3970
                     /locus_tag="VFV93_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX5165086.1"
                     /translation="MSEAFVAVVALLTIVAVLTRPRGINEGIWALGGGLLLAGTGRLS
                     LDSVGDVLRDLTGVLIFLIGLFWVTLAARRAGLFERAAHSVVTVSGGSGPWLMLAIFA
                     FGTLTTALLSNDATVVLVTPVVLSACLRLRIAPLPYLFACSFVADTASSLLPVSNPIN
                     LLYAERFDITFGSHLLLLGLPTIVAVIVNAGVFLLLFRSQLRPGYDLAAVHDWQIAPM
                     TTADQSIVAGLVLVGIGYIVAGLVGIQPSLVTFAGGVVLAAIAMAGSRVVPADLVQVQ
                     PPSLYAFVIGLALVVKAADVNGLLDTLGSAVSRANDAGGLSGLLAITFGTALGTNIVN
                     NWTMALAVTGPLARSDAGDLFVAGSMLGADIGPNLSVVGSLATLIWLTEVRRGGLAVS
                     GRTYLRLGLLTTIPTILAATITLYLVGRYA"
     gene            4000..4185
                     /locus_tag="VFV93_06805"
     CDS             4000..4185
                     /locus_tag="VFV93_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165087.1"
                     /translation="MDSATIIMIILVLVSLAAGLVTFGVLRERGRSSLVAGCGAIVIG
                     LIIVSCFVLFQIVRILT"
     gene            complement(4188..5807)
                     /locus_tag="VFV93_06810"
     CDS             complement(4188..5807)
                     /locus_tag="VFV93_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5165088.1"
                     /translation="MESRATTPHSPGVMLSALAGLATGAIVLASWWVLYSVNLLTFPP
                     FDLGDWFIRQSPGAFATWAIETLGDAAQSAALAGGVGIMLLAWALLGVGSGRLGDGWP
                     ARLLPLALLPLALALAWWTDDAGVGTAIWYTLVFGGTLLAGGAGLSHWLDRMWLATQS
                     AAPSSDYWLDRPGDYERRALMRQLAIATVALAGGGTILGRLLKGSSVGDVVTAQAMPL
                     ADARVALATPAALPTPQPAPPLPDGFSAPAGVRQRQTDNDEFYVVDISTRDPNLPEDG
                     WALRIHGLVERGLLISWPELLEMPPVELDGTLMCISYEHDNGLISTTRWTGVPLRDVL
                     QQAGVRDGAVELICRGANGYSDSIPLQKALEQTTLLAYGMNGTTLPRSHGFPCRLYVP
                     GLYGEKNVKWLEEIELVEYDYLGYWQERGWSDTATVNTVSIIDTPRGTVQRDNSGSVP
                     IGGIAFAGQRGISAVQVRVDDGQWQDAQLEANEPPLIWQRWRFDWQPDLGSYRLTVRA
                     VDGDGNPQDEQERPPHPDGMTGLHTVEVDVV"
     gene            complement(5853..7151)
                     /locus_tag="VFV93_06815"
     CDS             complement(5853..7151)
                     /locus_tag="VFV93_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5165089.1"
                     /translation="MRTDPRPVSKTAQRIVLGLVVFSFVFFAFAGGMLFQRYVIASDA
                     EAATGGAPATFDQVWDLVQTRFVDPKAINDDKMLEGAIDGMLATLKDEGHTRFLTAEE
                     SRSETESLQGEYVGVGIQVNERDDQIVVVAPIDGSPAQEAGVMAGDILISVDGVDVTG
                     QSVDQVVEKVRGEAGSSVTLRFLRDGQEAPLSYTLTRRKIDVSSVAWTMLDGNIALIR
                     LSQFSSGAGDDLARALEEAKADGATGVILDLRNNPGGYISEALQIGSMFVPEGDTIFI
                     SQVRDGTQEKHVATAQPQHIGDLPLVVLINEGSASSSEIVAGAVKANNPHASVIGETT
                     FGTGTVLSNFGLDDGSSLLLGTELWLTPDGSLIKNQGIRPDVVVGLPEGQFPFTPVDN
                     ADNDTSDIQDHQLDWAIHIITSGQAGDDHPTNGMPPTRAQ"
     gene            7705..8244
                     /locus_tag="VFV93_06820"
     CDS             7705..8244
                     /locus_tag="VFV93_06820"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX5165090.1"
                     /translation="MPFYTRTGDEGYTDLLGGRVPKYDTRPETYGTLDEATSNLGLAR
                     ALAQSDRTKELLVDVQRDLYLMMAELAFAPDMQQGKFHITAAHVERIEAATDALEADV
                     PLPPHFILPGDSVAGAALDVARAVVRRAERLAVKLAHDGEVTNDQILAYLNRLSSLLF
                     ILARFEDQVAGVTPTKAKG"
     gene            complement(8382..8735)
                     /locus_tag="VFV93_06825"
     CDS             complement(8382..8735)
                     /locus_tag="VFV93_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX5165091.1"
                     /translation="MDQYSDITLQCRDCGTDFIFTAGEQSFYAEKGFTNRPSRCASCR
                     AANKSRRDSSLSYSSGGNGGYGGGMRAERQMFPAVCDECGRETMVPFQPSGNKPVYCS
                     DCFASRRQQSAYPRY"
     gene            9037..9753
                     /locus_tag="VFV93_06830"
     CDS             9037..9753
                     /locus_tag="VFV93_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX5165092.1"
                     /translation="MQTARFGGFAAIVFDAYGTLFDVDGLDQRCAGVTDRPGDLIRIW
                     RMKQLEYAVLRTVMDRYADWGQITSDALDYAATALKLDLGPAARRTLTRAWLELPAFP
                     DVPPALDRLAGSDLRLLILSNGTRQMLDPLIAHNGLTGQFSSILTSESVQTFKPDPSI
                     YYQVTEIVHARINEILFVSSNGFDVAGAKSVGFTVCRVDRRGDPLDPLGFEPDLHVRD
                     LGELAHQLLGNDNNDGRKEG"
     gene            9759..10577
                     /locus_tag="VFV93_06835"
     CDS             9759..10577
                     /locus_tag="VFV93_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5165093.1"
                     /translation="MEWRDLGLSGLRVPVIGMGTWQTFDVYGKEDQASRRALVGDALD
                     HSIRFFDSSPMYGESERVLGTTLTGRRERAIVATKVWSHDLREGHGQIDRALLYFEGR
                     VDLYQVHNLVRWQDYLPVFERMKADGTIRASGITHYQPSAFRELVSIAQSEPIDTIQI
                     PYNPFERSVERAVLPVAAERQLGVIVMRPFGEGALLRKTPPAAALRPFRAFGCETWSQ
                     VLLKWILSDQRISVVIPATRDAAHLHANAVAGDGPWFGPAERSEVVELALRYCR"
     gene            complement(10978..11748)
                     /locus_tag="VFV93_06840"
     CDS             complement(10978..11748)
                     /locus_tag="VFV93_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165094.1"
                     /translation="MRWLMALLAFVLLSACSASSESGDSAPQRSAADLATVRAQAGIE
                     PPTPTMRPPSPTPTTPRPTATARTQQRNTGYATDESLYVALMSPDDISPMWTLGNDEG
                     IGLVELCGAAAIEDEFEPVGWAYGSYSASGGEWAEQWVIHLLEPDARAAMDYARASLT
                     CDEETFAIGSGGNDSILWEYSTLNLPEAGDDLYALGVTMTYSNPVYTPMNGSIVFVRQ
                     GEYVVVLLHYGFGIDLSVSAQMAQVAVARLDMIQDSSV"
     gene            complement(12320..12392)
                     /locus_tag="VFV93_06845"
     tRNA            complement(12320..12392)
                     /locus_tag="VFV93_06845"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12357..12359),aa:Lys,seq:ctt)
     gene            12569..13636
                     /gene="hemE"
                     /locus_tag="VFV93_06850"
     CDS             12569..13636
                     /gene="hemE"
                     /locus_tag="VFV93_06850"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX5165095.1"
                     /translation="MERTERFLRACRREPVDRTPVWFMRQAGRYMAEYRAIRAKHTLL
                     DIIANAELAAEVTLQPVEALGVDAAIIFADILPPLVPMGMDLEYAAGEGPVLRNPLRD
                     RAAIEALRSFDPETALTSTLDAISIVRRELDDKVALIGFAGGPFTLASYAIEGGSSRN
                     YQLTKSLMYRDPETWALLMDKLATMTAEYLNAQVRAGAQAVQIFDSWAGALAPDDYRR
                     YVLPATTQIVDALRPSGVPVILFGTNTSGMLDVIAEAGSSVVGVDWRIPLDRAWQIVG
                     HDRAVQGNLDPLVLFAPHNEVERHVQGILEQADGRPGHIFNLGHGILPQTPVETVQAV
                     VEMVHGVAVSGEFAAIGQRKR"
     gene            13715..14659
                     /gene="hemH"
                     /locus_tag="VFV93_06855"
     CDS             13715..14659
                     /gene="hemH"
                     /locus_tag="VFV93_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5165096.1"
                     /translation="MRTIGVMVMAYGGPGSLDEVGPYLADIRGGRPTSEELLHEITDR
                     YRQIGGRSPILELTTAQAQGIERALNDTAAAEAGIRYRVYVGMRHWHPYIDEVAGEML
                     ADGVDEVVGVVMAPHFSRMSIGAYMGKLDKALSDRGAQIPVHKVESWKDEPSFIEATA
                     ERVRQALLKFPEDIRAEVPILFTAHSLPARILESNDPYADELQESVRLVVKKLQPKDH
                     RWAFQSQGATAEPWLGPTVEDTLRQLADEGHRNVLLVPIGFVCDHVEVLFDVDIEHRH
                     QAEELGIRLERIEMLNDDPGLVNAVAAAVRRAAQTEAA"
     gene            14665..16080
                     /gene="hemG"
                     /locus_tag="VFV93_06860"
     CDS             14665..16080
                     /gene="hemG"
                     /locus_tag="VFV93_06860"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX5165097.1"
                     /translation="MTRIAVIGGGIAGLSAAWTLRQRDVDVTLFEAADRLGGKLQTER
                     IDDLLIDSGPDSFLSSKPAALQLIDALGLSSHVINTLADGGGTFVLRDGRLLPLPEGI
                     TLLVPAEFKSIARTPLLSPFGKARMVMDYVIPKRKDDADESIGAFVRRRVGKQAFERL
                     AEPLLSGIYAGDADQLSLLSTFPRLRETEQVHGSVIKGAIAQKRQARSTAPNAGPRRT
                     PFVSLSGGLSELIDGLADAIGREHIQTGAHVSSIVELENEVAVELASGAREVFDGVVI
                     AAPASETADMLAQLDAGLTAELRRIPYVSSATVSLAFDIADVADKQFGRGFVIPRAEG
                     RTLTAVTWTSNKFGGRVPDGVALLRGFVGRAGNQRPAHLADDELIPLVRAELQAILGI
                     DAEPLLARVYRWPRALPQYNLGHPDLLASLAERLASHPGIALTGAAYRGVGIPDCISD
                     ATTQANALADRIGASVAAGTA"
     gene            16319..16504
                     /locus_tag="VFV93_06865"
     CDS             16319..16504
                     /locus_tag="VFV93_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165098.1"
                     /translation="MQSALPNACDIDSRSEVNDSQCRQIDEETSTIRRWINDALDWLD
                     RYWDDPELRSGFWHSGW"
BASE COUNT         2860 a         5555 c         5394 g         2772 t
ORIGIN      
        1 ccgacctgca acgccgcgtc gaggagctcc agcgcgagca gacggagttc accgaggatg
       61 cggagcggca cagcaacgcg gctgtcgagg tccgggcgcg cctcagcgat atcgaagcac
      121 agctcgccga tcacgccaga atggccggcg ccgctcgcca gcgccgcgcc gacctgcggg
      181 cagccatcga ggctgccgag gcccgactgg ttacgattcg ccgtgagctg gcgggaatcg
      241 agggcaccca gacctcgatc gccgaacgta tcgcgacagt cgccgccgac cgcgaacgcg
      301 tcagcacaca gactgccaac gaacgggaac agttcgaccg gttgcagcac agcgttgccg
      361 atctggatgc acagatcgcc cgggtcgcag agcgcgagac ggcgatccag gcgtcgatcg
      421 cggagcatct ggcgagtgcc acaacggccg cggacggact ggctgagcgg cgcgccgcgc
      481 tccagacgat cgagcgtgag gtgacaaacc ttcagacgcg gctcgacgcg ctggaacgag
      541 cgcatgccag cggcgagggg ttgtacgccg gtgtccgggc catcctgcgg gcaatcgatc
      601 gcagccagtt gttactgcct gggctggtcg gcacgctggc ggagaccgtc gaggtgccgg
      661 ccgaattgga aacggcgatc gaggtcgccc tcggcggtca ccttcaagac ctgatcgtcc
      721 agacgtggtc ggacgcgcag gaggcgatcg cgtttctgaa acggacgaac gccggccgtg
      781 ccacgttcca gccgctcgat accgtccgtc ccgtccggcg accggcgctg agtgcgagcg
      841 acggcgacct gcgcggtgtc gctgccgacc tggtcacgtt ccctgaacag gtcgcgccgg
      901 tcgtggagca gttgcttggt cgcacgctgg tagtagccga tctggatgcc acccggcgaa
      961 tcctgaaatc agcctcaggc tggacgattg tgacggtcgg cggcgagatc gctcgtccgt
     1021 ccgggagtgt gaccggcggc gggcgcgcgg ccgaatcggg catcctcgcc cgcgagcgcg
     1081 agcgccgtgc gctgccggcg acgctcgcgg cccgcaacgc gacccgcgcc gaggtccaag
     1141 ctaccatcga gcgcgccgtc gccgaggtcg agaaacgcga gaccgcccgg cgaacgctgc
     1201 aatcggagct tgaatcggtc agcaggcagt tacgagaatt gcgggcagat cgggagcaac
     1261 agtctcgaag ccgcgatgcg actctggccg cgctccagca ggcagatagt cgcgctgaaa
     1321 ccctcgaaca gcgcgaacag ggcttgcggc aggaatccgc aaccctggag tcccgcacgg
     1381 cggaactgga agctgagcag cgcggcgccc gtgaggaacg ggcccgcgtc gaggcgctgc
     1441 tcgctgccga acccgaggat gtcgatgcgg cggctgccgg gctgcgtgtc gaactggcgg
     1501 cggatcagga acgcctgcat acggccgagt tatcagcccg gaaagcccgc gagcgcgccg
     1561 aatccgccca gcgttcggca aacgaacgca ccgccgagat cgaacggctc agggaagcgg
     1621 aaaccgacga tgcgcaacgg ctgcgtaagt tcgacgcgca gatcctcgaa ttcgagcaac
     1681 tcgtcgccac cggccaggcg gcgctcccac cgatcgccca gcaacgggat gaggtcgtcg
     1741 agttgctggc gacggccgag cgtacgctca gccgcgaggc cgctggcctg cgcgaggcgg
     1801 agcgagagcg cgaccggcac agcctcaacc tggcgcgcgt ccaggacgag caggtctttc
     1861 tcgtcgagcg catccgcagc gacctggatc tggacgatcc gtccgatctg gaagcgattg
     1921 aagacgacgc gccggttccc gacgaggggg agatcaaccg gctccgtgat cggctgcgcc
     1981 gcatgagcaa cgtcggcgag gatgtcctcg aacagcacga cgccgaatca cagcgtctca
     2041 cctacctgtc cggccagctg gccgacatcg acgaggcgtc ggacgggctg cggcgcgtgc
     2101 tgtccgagct gaacagcaag atggcgacgc gcttcaccga gacgttccgc gaggtggcgg
     2161 tcgccttcga gcataccttc acgcggctgt ttggcggagg ctcggcccgg ctcgtgatga
     2221 gcgccaatga ggacggcgcg gccggtatcg acatcgtcgc ccaaccaccc ggcaagcggc
     2281 tgcagaacct gaacgctctt tcgggcggtg agcgggcatt gacggccgtc gcgctgctga
     2341 tcgcgatcca gcgcgtcaac cccagcccgt tctgcctgct cgatgaggtc gacgccgcgc
     2401 tcgacgagtc caatgtcgtc cgcttccgcg acgagttgcg cgacctggcc ggcaacaccc
     2461 agtacgtcgt tatcacgcac aatcgcggaa cgattgaggg cgccgacacg ctctacggcg
     2521 tcacgatggg tgacgacggc gtctcacgcg ttctctcgct ccgactcgaa gaggcgatcc
     2581 aggccgtcga agcgtacgag agcgcccagc ctgccggcac ctgatcggcg ggcgaccatg
     2641 ctagcatcgc ggctgccggc ggactggccg gcgttgtcga cagctgcgtg gacgagtggc
     2701 cgtgtcggaa gcgtttgttg cagtcgttgc gctcctgacg atcgtcgccg tcctgacacg
     2761 tccgcgtggc atcaacgagg gcatctgggc gctcggcggc gggctgctgc tggcgggaac
     2821 aggtcggctg tctctcgatt ccgtcggtga cgttctgcgc gatctgaccg gcgtgctgat
     2881 ctttctgatc ggtctgttct gggtcacgct ggcggcccgg cgggccggtc tgttcgagcg
     2941 agccgcgcac agcgtcgtga cagtctcggg cggcagcggt ccttggctga tgctggccat
     3001 cttcgcgttc ggcacattga cgaccgcgct gctctccaac gacgccaccg tcgtcctcgt
     3061 cacgcccgtc gtgctgagtg cctgcctgcg gctcagaatc gcgccactgc cctacctgtt
     3121 cgcctgctcg ttcgtggccg acaccgcgtc cagcctgctg ccggtctcca acccgatcaa
     3181 cctgctctac gccgaacggt tcgacatcac cttcggctcg catctgctct tgctgggctt
     3241 gccaaccatc gttgccgtca tcgtcaatgc cggtgtcttc ctgttgctgt ttcgctcgca
     3301 gttgcggcca ggctacgacc tggcagccgt ccacgactgg cagatcgcgc cgatgacaac
     3361 cgccgaccaa tcgattgtgg cggggcttgt cctggtcggg attggttaca tcgtcgcggg
     3421 tctggtcggg attcagccgt cgctggtgac gttcgccggg ggcgtcgtcc tggcggccat
     3481 cgcgatggca gggagccggg tcgttccggc cgatttggtg caggtccagc cgccgtcgct
     3541 gtatgcgttc gtgatcggcc tggcgctggt cgtcaaagcc gccgatgtca acgggctgct
     3601 cgacacactg ggtagcgccg tcagccgtgc gaacgacgcc ggcgggctca gcggtctgct
     3661 ggcgatcacg tttgggacgg cgctcggcac gaacatcgtc aacaactgga cgatggcgct
     3721 ggcggtcact ggaccgctgg cgcgctcgga tgccggtgat ctgttcgtcg cagggtcgat
     3781 gctgggagcc gacatcgggc caaacctgtc ggtcgtcgga tcgctggcaa cgctgatctg
     3841 gctgaccgaa gtgcggcgtg gcggactggc cgtctccggc cgcacttatc tccggctggg
     3901 gttgctgacg acgatcccga cgatcctggc cgcgaccatc acgctctacc tggtcgggcg
     3961 atatgcatga cggtggcggg cgtgcaatga tatgcgggta tggatagcgc gacaatcatc
     4021 atgatcatcc tggtcctcgt ctcgctggcc gccgggctgg tgacgtttgg cgtcctgcgt
     4081 gagcgcgggc gcagttcgct ggtggccggt tgcggggcaa ttgtgatcgg cctgatcatc
     4141 gtcagttgct tcgtgctgtt tcagatcgtc cggattctca cctgacctca gacgacatcg
     4201 acttcgacgg tgtgtagacc cgtcatccca tccgggtgcg gcggtcgctc ctgctcgtcc
     4261 tgggggttgc cgtctccgtc aacggcccgt accgtcagcc gatagctgcc cagatccggt
     4321 tgccagtcga agcgccagcg ctgccagatc agcggcggct cgttcgcttc caactgcgcg
     4381 tcctgccact gcccgtcatc gacgcgcacc tggacggcgc tgatgccacg ctgaccggcg
     4441 aacgcgatcc cgcctatcgg gacgctccca ctgttgtcgc gctggacagt cccgcgtggc
     4501 gtgtcgatga tcgagaccgt gttgacggtg gccgtgtcgc tccagccgcg ctcctgccag
     4561 tagccgaggt agtcgtactc gaccagctcg atctcctcca gccacttgac gttcttctcg
     4621 ccatacaacc ccggcacgta gagcctgcac gggaagccgt gcgagcgcgg caaggtcgtg
     4681 ccgttcatgc cgtaggcgag caacgtcgtc tgctccagcg ccttctggag tggaatcgag
     4741 tccgagtagc cgttcgcgcc acggcagatg agctcgaccg ccccatcccg tacacctgcc
     4801 tgctggagga catcgcgcag cggcacacct gtccagcgcg tcgtgctgat gagtccgttg
     4861 tcgtgctcgt acgagatgca catcaacgtt ccgtccagct cgactggtgg catctccagc
     4921 agctccggcc agctgatcag cagcccgcgc tcgacgagtc cgtggatccg caatgcccag
     4981 ccatcttcgg gcaggttcgg atcgcgggtc gagatgtcga cgacgtagaa ctcgtcgttg
     5041 tccgtctgtc gttgccggac gccggccgga gcgctgaagc cgtccggcaa gggcggcgcc
     5101 ggctgcggcg tcggcaaggc agccggtgtc gccagcgcga cccgcgcgtc cgccagcggc
     5161 atcgcctggg cggtcaccac atcaccaacg ctgctgccct tcagcagccg gccaaggatc
     5221 gtcccgccgc cggccagcgc gaccgtcgcg atcgcgagtt gccgcatcag tgcccgccgt
     5281 tcgtagtcac cgggccgatc gagccagtag tccgaagatg gcgcggctga ttgcgtcgcc
     5341 agccacatcc ggtccagcca gtgcgacaac ccagcgccac cggccagcag cgtgcctccg
     5401 aagaccagcg tgtaccagat cgccgtcccg acaccggcgt catccgtcca ccacgcgagg
     5461 gccagcgcga gcggtagcag cgccagcggc aacagcctgg ccggccagcc atcgccgagc
     5521 cggcctgacc cgacgccgag cagagcccag gcgagcagca tgatgccaac cccgccggcc
     5581 agcgcggccg attgggcggc atcgccaagc gtctcgatgg cccaggttgc aaacgcgcca
     5641 ggcgactggc ggatgaacca gtcgccgagg tcgaacggcg ggaacgtcag caggttgacg
     5701 ctgtacagca cccaccagct cgccaggacg attgcgccgg tcgccagtcc ggccagcgcc
     5761 gagagcatga cgccaggcga atgtggtgtg gtcgcgcgcg actccatcgt tgcggatcct
     5821 ctggccgatg gacgcccagc ccggaacgcc ccttactgcg cccgggtcgg cggcatcccg
     5881 ttcgtcggat ggtcgtcgcc ggcctggccg ctggtgatga tgtggatggc ccagtcgagc
     5941 tggtggtcct gaatgtcgct cgtgtcgttg tcggcgttgt cgaccggcgt gaaggggaat
     6001 tgtccttcgg gcaggccgac gacgacatct ggccggatcc cctggttctt gatcagcgag
     6061 ccatccggcg tcagccagag ttcggtgccg agcaacaggc ttgacccgtc atcgaggccg
     6121 aagttgctga ggacagttcc agtcccaaac gtcgtctcgc cgatgacgct cgcgtgcgga
     6181 ttgttggcct tgacagcgcc cgccacgatc tccgacgacg acgccgagcc ctcgttgatc
     6241 agcacgacca gcggcagatc gccgatgtgc tgtggctggg ccgtcgcgac atgcttctcc
     6301 tgcgtgccgt cgcgcacctg cgagatgaag atcgtgtcgc cctcagggac gaacatcgag
     6361 ccgatctgca gcgcctcgct gatgtagcca cccggattgt tacgcaggtc gaggatgaca
     6421 cccgttgcgc catccgcctt ggcctcctcc agcgcccgtg ccagatcgtc accggcgccg
     6481 gacgagaact gcgacaggcg gatcagggcg atgttgccgt ccaacatcgt ccaggcgaca
     6541 gacgagacat cgatcttgcg ccgcgtcagt gtgtacgaga gcggcgcctc ctgaccgtca
     6601 cgcaggaagc gcagagtgac ggagctgccg gcctcgccgc gtaccttctc gacaacctga
     6661 tcgaccgact ggccggtgac gtcgacccca tcgaccgaga tcaggatatc gccggccatc
     6721 acgccggcct cctgcgccgg cgatccgtcg atcggcgcca cgacaacgat ctggtcgtcc
     6781 cgttcattga cctggatacc gacgccaacg tactcgccct ggagactctc cgtttccgag
     6841 cgcgactcct cagccgtcag gaagcgggta tgtccctcgt ccttgagggt cgcgagcatg
     6901 ccgtcgatcg cgccttcgag catcttgtcg tcattgattg ccttcgggtc aacgaagcgg
     6961 gtctggacga gatcccagac ctgatcgaat gtcgccggag cgccacccgt cgccgcttcg
     7021 gcgtcgctgg cgatgacgta acgctggaag agcatcccac ccgcgaatgc aaagaagacg
     7081 aagctgaaaa cgaccagccc gaggacgatc cgctgggccg tcttcgagac ggggcgggga
     7141 tcggttcgca cggcagttcg tgcctcccgc tcttcgccag cggcgggaag cggttcgacg
     7201 ggcggtgttg cagaacgttc tcgcggaaat tgaccgccgc tggccggcgt ctcggttgaa
     7261 tccatgaggg catcatttcc atgcatcagg tgaaggcgcc cggcggcagg cggaactgcc
     7321 gcctgggtat ggacgctgtt gagaatggac agtgtatggc cccggcagta cagcccggcc
     7381 cacgctttca aaagtatacg tgtgccgctg aaagcgcgat gacgtggggc gcgatgcgtt
     7441 ttcaggagga tttcaggttt ctgtcacacg gcgctcaggt tcagggccaa tactgctatc
     7501 agcgtcacaa ggacgcttcg cacgaacacc acccccaaca cccccaacac gctgatcccg
     7561 cgtgtatcag ggatcccacc ccccaacgac ggcccgccgg aagcgcaacc ggcgggccgc
     7621 cctttttccg cggccattgc ggcaggaaca cgcagccggt acggtttcag cgaggcagtt
     7681 gcgagcaagc gctgggagca ccggatgccg ttctacaccc gcacaggcga cgagggatac
     7741 accgatctac tgggcggccg cgtgccgaag tacgacaccc ggcccgagac gtatgggacg
     7801 ctcgacgaag cgacgtccaa tctcgggctg gcgcgggcgc tggcgcagtc cgaccgcaca
     7861 aaggagttgc tggtcgatgt ccagcgcgac ctgtatctga tgatggccga gctggccttc
     7921 gcgccggaca tgcagcaggg gaaattccac atcacggccg cccacgtcga acggatcgag
     7981 gccgccaccg acgccctcga agccgacgta ccgctgccgc cgcacttcat cctgccgggc
     8041 gactcggtcg ccggcgcagc gctggatgtt gcgcgggccg tcgtccgccg cgccgagcgg
     8101 ctggccgtca agctcgcgca cgacggcgag gtcaccaacg accagatcct cgcttacctc
     8161 aaccggctct catcgctgct cttcatcctg gcgcgcttcg aggatcaggt cgccggcgtg
     8221 acgccgacga aggcgaaggg gtgattttcg ggacgcggga cgcgggacgc gggacgcggg
     8281 acgtgatgga tgaaatgtct ccgcgtcacg cgtcacgcaa aacacaaacg gggccggcgc
     8341 aatcggcccc gttcgtatgc ttgtcgaggt tgtgacgcgg cttagtagcg cgggtatgcg
     8401 gactgctggc gccgggaggc gaagcagtca gagcagtaga ccggcttgtt gccgctcggc
     8461 tggaacggca ccatcgtctc gcgaccgcac tcgtcgcaga cagccgggaa catctggcgc
     8521 tcggcgcgca tgccgccacc atagccacca ttgccgccgg acgagtagct cagcgatgaa
     8581 tcgcggcgcg acttgttggc cgcgcggcac gacgcgcacc ggctcgggcg gttcgtgaac
     8641 cccttctcgg cgtagaagct ttgctctcca gccgtgaaga tgaagtccgt tccgcagtca
     8701 cggcactgaa gcgtgatgtc ggaatactgg tccatgtgat aagaactcct tggcgcacgt
     8761 cgtcatccgc tgacgacccc caacgtaccc atcgtcgaac tgacatccat gcgccgcgag
     8821 gctgaggcga gggatactcg gacacttcca cctatggcga ttgcagttga cgtgtgccta
     8881 taaataacat actttgcgcg gtagcgctag ccccgtccga aaatcgcccg catttcgacc
     8941 acaacgacgg ctgccgcgac catccatcgt ccctgtccag tgcacgctcg cccagatcag
     9001 ggcctgccct gcctttcgcg aagtggaagt tgacccatgc aaaccgcacg cttcggtggg
     9061 ttcgcggcca tcgtcttcga cgcctatggg acgctcttcg acgtcgatgg tctcgaccag
     9121 cgttgcgccg gcgtgaccga tcggccgggc gacctcattc ggatctggag gatgaagcaa
     9181 ctcgaatatg ccgtcctgcg gaccgtcatg gatcgctacg ctgactgggg ccagatcacc
     9241 tcggacgccc tcgactacgc cgcgacagcc ctgaagctcg acctcggtcc ggccgcccgg
     9301 cgcacgttga cccgcgcgtg gctggagcta ccggcgttcc cggatgtgcc gccggccctt
     9361 gaccgcctgg ccggctccga tctgcggctg ctgatcctct ccaacggcac caggcagatg
     9421 ctggacccgc tgatcgccca caacggactg accggccagt tctcgtccat cctgaccagt
     9481 gaatcggtcc agaccttcaa gcccgaccca agtatctact atcaggtgac agagatcgtc
     9541 cacgcccgaa tcaacgagat cctgttcgtc agctcgaatg gcttcgacgt ggccggcgca
     9601 aagtcggtcg gcttcacggt ctgccgggtc gatcggcgtg gcgacccgct cgatccgctc
     9661 gggttcgagc cggatctgca tgtgcgcgac ctgggcgagc tggcccacca actgctgggc
     9721 aacgacaata acgacgggcg aaaggaaggc tgatcgagtt ggaatggcgc gacctcggat
     9781 tgagcgggtt gcgagtgccc gtgatcggta tgggtacgtg gcagacgttc gatgtctacg
     9841 gcaaggaaga tcaggccagc cggagggcgc tcgtcggtga cgcgctcgac cactcgatcc
     9901 gtttcttcga ctcatctccg atgtatggcg aatccgaacg ggtcctcggg acaacgttaa
     9961 ccggccggcg cgagcgcgcg atcgtcgcca ccaaggtctg gtcccacgat ctgcgcgaag
    10021 gtcacggcca gatcgaccgg gcgctgctct acttcgaggg ccgcgtcgac ctctatcagg
    10081 tccacaacct cgtccgctgg caggactacc tgccggtctt cgagcggatg aaagcggacg
    10141 gcacgatccg cgcgtccggc atcactcact accagccgtc cgcgttccgt gagctcgtct
    10201 cgatcgccca aagcgaaccg atcgacacga tccagattcc ctacaacccc ttcgaacgct
    10261 cggtcgagcg agcggtcctg ccggtcgcag ccgagcggca gctcggggtg atcgtcatgc
    10321 ggcctttcgg cgagggagcg ctgctccgta agacgcctcc ggcagcagcg ctccgcccgt
    10381 tccgggcctt cggctgtgag acctggtcac aggtgctgtt gaagtggatt ctgagcgacc
    10441 agcgcatctc ggtcgtcatc cccgcgaccc gggacgccgc ccacctgcac gccaatgccg
    10501 tcgccggcga tggtccgtgg tttgggccgg ccgaacgctc cgaggtcgtc gagttggccc
    10561 tgcgctactg tcgctgacgg ctgcgacctg actccggcaa agggccggga cgcccgctgg
    10621 tcattcgtac aggctcaaat caggccctac taacagcgcg gacagcacgg cgcttcgcta
    10681 tcctgatgca tgtcggaggt cggcaatggg gccggccaga ccgcaacgat gcgggccggc
    10741 gagagcaaga cgtcgacgac acgacgccgt gaaggagggc caacgatgag tccatatctg
    10801 atggagaaga tcgtcgagca caaccagaac gagctggccc gcgcacggca gaagcgcgaa
    10861 tggcgctcac tgttccgcaa gtagcgcgga acagcccaac tcgatcacta ctcccatcca
    10921 tccctttctc cggccgggcg agcaatcgtc cggtcactct tgtgtctggg agtcgagtta
    10981 aacgctgctg tcctgaatca tgtcgaggcg cgcaacggcc acctgcgcca tctgggccga
    11041 taccgacaga tcaattccaa acccatagtg cagcagcacg accacgtact cgccctgccg
    11101 gacaaacacg atcgagccgt tcatcggcgt gtagaccggg ttggagtagg tcatcgtgac
    11161 gccgagcgcg tagagatcgt cgccggcctc cggcaggttc agcgtgctgt actcccacag
    11221 gatgctgtcg ttgccacctg agccgattgc gaacgtctcc tcgtcgcagg tcagtgacgc
    11281 tcgggcgtag tccatcgccg cgcgagcatc cggttccagc aggtgaatca cccactgttc
    11341 cgcccactcg ccgccagagg cgctgtacga gccatacgcc cagccaaccg gctcgaactc
    11401 gtcctcgatc gcggcggcgc cgcacaactc gacgaggccg atcccctcat cgttgcccaa
    11461 cgtccacatc ggcgagatgt catccggcga catcaacgcg acatacaggc tctcgtcagt
    11521 cgcgtagccg gtgttgcgct gctgcgttcg ggccgttgcg gtcggccggg gcgtcgtcgg
    11581 cgtcgggctg ggaggacgca tggttggcgt cggcggctcg atcccggcct gtgcccgcac
    11641 cgtcgcgaga tcggccgcac tccgctgcgg tgcggagtcg ccgctttcag aactcgcgga
    11701 acaggccgag aggaggacga acgcaaggag tgccattaac cagcgcatac gagttcatgc
    11761 tccagatcca ccgagaccgg tcgagacgca tgcacaaacg atgacacccg aaccagcgtc
    11821 cgtccagatc agacgcgtgg tatttcagtc atgaatccag cccccgttgt cgtccttcgg
    11881 ctgccgcctc aggatgacaa cgggcagggg gcctacacat catcacgaca tggatagcaa
    11941 cgacatcttg attgaggtcg gtcatgctgg aaaccgcgcg actgcaacgc acgatcgggc
    12001 ggagcggtcg cacgcaagcc cccgagggtg cccagccctc ggggcttgct ccaccatttc
    12061 tcaaccagtc cgcgacccct tcgcgaccgg tccgggcgac gatcaggtat ccgaaaccca
    12121 agaatcccgt gcagtcagat cgaggccccc accttcgatt gccgggacac gccccacccc
    12181 tcctcggctg aaccggctcg tcaccctggc cggttcagcc acctcgccac atcccgatcc
    12241 ggacagcgac cgaaccgcca gacaacaaga caggcggtct cgcgaccgcc cggacatcac
    12301 catcctgtgg ttacctgtgt gagccgccag ggactcgaac cccgaacccg ctgattaaga
    12361 gtcagctgct ctgccaattg agctagcggc ccgcgctgtt tccggcgcga aggccatttt
    12421 agcgtagatg cgctcagatt tccagtcttg tgccgaaatt cacgatcccg cgttttgcgc
    12481 ggccgggatt gataagattc gccgtggcga cgtcgtccgg caggcatcgc cgcctgcggg
    12541 actgccacag cgaagggatg acgaggtctt ggaacgtacc gagcgctttc tgcgcgcctg
    12601 ccgtcgtgaa ccggtcgacc ggacgcccgt ctggttcatg cgacaggccg ggcgctacat
    12661 ggccgagtat cgcgccatcc gcgccaagca cacgttgctc gacatcatcg ccaacgccga
    12721 gcttgccgcc gaggtgacgc tccaaccggt tgaggcgctc ggtgtcgatg ccgcaatcat
    12781 cttcgccgac atcctgccgc cgctggtgcc gatgggcatg gacctggagt acgcggccgg
    12841 tgaaggcccc gttctgcgca acccactgcg tgaccgggca gcgatcgagg cgttgcggtc
    12901 attcgatccg gaaacggcgc tgacgtcgac gctggatgcg atctcgatcg tccggcgcga
    12961 actggacgac aaagtcgcgc tgatcgggtt cgccggcggg ccgttcacgc tcgccagcta
    13021 cgcgatcgag ggcgggtcgt cgcgcaacta ccagctgacc aagtcattga tgtaccgcga
    13081 cccggaaacc tgggcgctgc tgatggacaa gctggcgacg atgacggccg agtacctcaa
    13141 cgcacaggta cgggccggcg cacaggcagt ccagatcttc gattcctggg ctggcgcgct
    13201 ggcgccggat gactaccgcc gctacgtcct gccggccacg actcagattg tcgatgcgct
    13261 gcggcccagc ggcgtgccgg tcatcctgtt tggcaccaac accagcggaa tgctggatgt
    13321 catcgccgag gctggaagca gcgtcgtcgg cgtcgactgg cggattccgc tcgaccgggc
    13381 ctggcagatc gtcggccacg accgggccgt ccagggcaac ctcgacccgc tcgtcctctt
    13441 cgcgccgcat aacgaggtcg agcggcacgt gcagggcatc ctggaacagg ccgacggccg
    13501 gccggggcac atcttcaacc tcggccacgg catcctgccg cagacaccag tagagacggt
    13561 ccaggccgtt gtcgagatgg tgcatggggt agctgtcagc ggcgagttcg cagctatcgg
    13621 ccagcgaaaa cgatgatgga agtacgcgtg gatgtcgaca gctcggcggc aggagaccgt
    13681 tggctgccgg ccgcaagcta accgggagat acgcatgcgg acaatcggtg tgatggtgat
    13741 ggcgtacggc ggaccgggat cgctcgacga ggtcgggccg tatctggccg acattcgcgg
    13801 cggccggccg acgtccgagg agctgctgca cgagatcacc gaccgttacc gccagatcgg
    13861 cgggagatca ccgatcctgg agctgaccac cgcccaggcg cagggaatcg aacgcgccct
    13921 gaatgacacg gccgcggccg aagccggtat ccgctaccgc gtctacgtcg gcatgcgcca
    13981 ctggcatcca tacatcgatg aggtcgccgg cgagatgctg gcggacggcg ttgacgaggt
    14041 cgtcggcgtc gtgatggcgc cgcacttcag ccggatgtcg atcggcgcct acatgggcaa
    14101 gctcgacaag gcgctgtccg accgtggcgc gcagatcccc gtccacaagg tcgagagctg
    14161 gaaggacgag ccgagcttca ttgaggcaac ggcggagcgg gtgcgccagg cgctgctgaa
    14221 gttccccgaa gacatccgcg ccgaggtgcc gattctcttc acggcccact cgctgccggc
    14281 ccgcatcctc gaatcgaacg atccgtacgc cgatgaattg caggagtcgg tccggctcgt
    14341 cgtcaagaag ctccaaccga aggaccaccg ttgggcgttc cagagccagg gcgcgaccgc
    14401 cgagccgtgg ctgggaccga ccgtcgaaga cacgctccgg caactggccg acgagggaca
    14461 caggaatgtc ctgctggtgc cgatcggctt cgtctgcgac cacgtcgagg tgctgttcga
    14521 tgtcgacatc gagcatcgcc accaggccga ggagttgggg atccggctgg agcgcatcga
    14581 gatgctcaac gacgatcccg gcctcgtcaa tgccgtcgcc gccgccgtcc gccgcgcggc
    14641 ccagaccgaa gcggcctgat cgagatgacg cggatcgctg tcatcggtgg cgggattgcc
    14701 ggcctgagcg ctgcctggac gctgcggcag cgcgacgtcg atgtcaccct cttcgaggcc
    14761 gctgaccggc tcggcggcaa gctccagacc gaacggatcg acgatctgct gatcgacagc
    14821 ggcccggact cgttcctctc atccaaaccg gctgcgttgc agttgatcga cgccctcggg
    14881 ctgagttcac acgtcatcaa cacgctcgcc gatggtggcg gaacctttgt cctgcgcgat
    14941 ggccggctgt tgccgttgcc ggagggaatt acgctgctgg tgccggccga gttcaaatca
    15001 atcgcccgaa caccactcct ctcaccattc ggcaaagcgc gaatggtgat ggattatgtc
    15061 attccgaagc ggaaggatga cgccgacgag tcgatcggcg cgttcgtccg tcgccgggtc
    15121 ggcaagcagg cgttcgagcg gctggccgag ccgctactgt ccggtatcta cgccggcgac
    15181 gctgaccagt tgagtctgct ctcgaccttc ccccgcctgc gcgagaccga acaggtgcat
    15241 ggtagcgtca tcaagggcgc gatcgcccag aagcggcagg cgcgctcgac cgcaccgaat
    15301 gccggaccgc ggcggacgcc gttcgtctcg ctgagcggcg ggttgagcga gttgatcgac
    15361 gggctggccg acgcgattgg ccgcgagcac atccagacgg gcgcccacgt ctcatcaatc
    15421 gtcgagctgg agaacgaggt ggccgttgag ctggccagcg gcgcgcgcga ggtcttcgat
    15481 ggtgtcgtca tcgccgcccc ggccagcgag acggccgata tgctggccca gctcgacgcc
    15541 gggctgacgg ccgagctgcg tcgaatcccg tacgtctcca gtgcaacggt ctcgctggcc
    15601 ttcgacattg ccgatgtcgc cgacaagcag ttcggacggg ggttcgtcat cccgcgcgcc
    15661 gaggggcgga cgctgacggc cgtcacctgg accagcaata agttcggcgg gcgcgtgccg
    15721 gatggtgtcg cgctgctgcg tggcttcgtc ggtcgtgccg gcaaccagcg cccggcccac
    15781 ctggcagacg acgagttgat cccgctcgtc cgggccgagc tacaggcgat cctcggcatt
    15841 gacgccgagc cgctgctggc gcgcgtctac cgctggcccc gcgcattacc gcagtacaat
    15901 ctcggccatc ctgacctgct ggcctcgctg gcggagcggc tggcgtcaca tcccggcatc
    15961 gcgctgacgg gcgccgccta ccgcggcgtc ggcatccccg actgcatcag cgacgcgacg
    16021 actcaggcca atgcactggc cgatcggatc ggtgcctccg tcgcggccgg aacggcttga
    16081 cgatgcgtgg cgcattcact aggctgcgca gcatgagcac acgtgcgacg accgaccgct
    16141 atccagcctc tcgaccggca gcacggacgt tcctcgtcta catgccgtcc ggtggccgct
    16201 ggccgcgcat ctgcgcctgt acctgctcct gatccacccc tgacaagcaa tcgtcgtcac
    16261 gcatcgtcgc gcgatcagtg cgcccggtcg cgttgacgcc gccggacagg agcaatccat
    16321 gcaatccgcg ctaccgaacg cgtgcgacat cgacagtcgc agcgaagtga atgacagcca
    16381 atgccgacag atcgacgagg agaccagtac catccgtcgt tggatcaacg acgcgctcga
    16441 ctggctcgac cgctactggg acgatccgga gctacgctcc ggcttctggc attcaggctg
    16501 gtagcgaaga aacgttccac cccacgtagg ggcgacggcc gcagcctcgc catgcccggt
    16561 gcgtgcgaaa cgcgggcgag g
//
LOCUS       DASUGJ010000311         6997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1296851, whole genome shotgun sequence.
ACCESSION   DASUGJ010000311 DASUGJ010000000
VERSION     DASUGJ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296851"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VFV93_06870"
     CDS             complement(<1..524)
                     /locus_tag="VFV93_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5165099.1"
                     /translation="MSQRPAWESYLDEHHDQHLEELFELLRIPSISALPDHRDDVRKA
                     AEWVADALRKVGVPKVEVMPTNGNPVVYGEWIVDASKPTALIYGHYDVQPPDPLDLWE
                     TPPFEPTIRDGMIYARGASDDKGNLFMPIKAIEALVQTQGAPPINLKFTIEGEEEIGS
                     PNLPLFIREQKELLA"
     gene            925..1458
                     /locus_tag="VFV93_06875"
     CDS             925..1458
                     /locus_tag="VFV93_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5165100.1"
                     /translation="MSESSMLGVVIRQRRIELGWTQEELAERISSDGEYVRQSEISRI
                     ESGKVGLPRHERLVRLADVLDLPLGELLARSGWTGADQHFHVADGDPTATNHTDELVS
                     TVPMEGMPARKRRQPVNETDASAMPALRRAVSTMRDESERLNRNCERSLELVEQVRRS
                     GSIRIRTHDDYLDPSAT"
     gene            complement(1434..2522)
                     /locus_tag="VFV93_06880"
     CDS             complement(1434..2522)
                     /locus_tag="VFV93_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX5165101.1"
                     /translation="MARRDIVTIGASAGGVEALMTIARHLPADLPAAVFVVLHIPPDG
                     TSALPTLLSRAGQLPAEHPDDGEPVRHGRIYVAPPDHHLLLHDGRVRVVHGPRENRHR
                     PSVDPLMRSAAQFYGPRAIGVILSGSLDDGTAGLLALKRQGGVAIVQDPAEAAFPGMP
                     SSAMHVVKVDYCLRVARIGPTITRLVTEPIEETEAAAMSSRGDDRMKYENSISEVDQS
                     ALENDDRPGIPSPFACPECGGVLWEVENAQMLRFRCRVGHAYSVESLLASQNEGMEAA
                     LWSAMRALEEKASLTRRLADRAEEQSQHGAAERFAEQSKAAEEHANTIRAVLLSENAT
                     VPASTRSHHPRVNARKQDASTQVAEGSR"
     gene            2801..6112
                     /locus_tag="VFV93_06885"
     CDS             2801..6112
                     /locus_tag="VFV93_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX5165102.1"
                     /translation="MAEQRQGDSIVVIGASAGGIDALSTLVTTIPANLPAPIVVAQHL
                     DPRRPSRLNDLLSRRSTVPIRTVEDRAALEPGVVYLVPENSDVEIADHQVMVRRDGQR
                     TPLPSIDLLFTTAAASYGENTIAVILTGTGTDGAAGARDVKASGGTIVVQNPETASFP
                     GMPLSLAPSIVDIVANLESIGALIGDLLSGTYPISRPDEDLHLHGLLEQLRERSGIDF
                     NAYKAPTITRRLQRRMAATGASSLSDYMRYLQRNPDEYQRLINSFLIKVTEFFRDPEL
                     FEYLREQVLPAVVEEGRERGELRLWSAGCATGEEAYSLAILMAEQLGDELDSFNARIF
                     ATDLDADAVGFARRGIYPSTALATLPPEMVERYFTRTDGAYEVRKSVRSLVVFGQHDL
                     GQRAPFPRIDVTLCRNVLIYFTADLQKRALQLVAFSLRDQGYLILGKAETVSPLPEYF
                     SLEQPRLKVYRRVGERVLIPTARIRDSVPTLPIRVTPPRIHGADGAALRLRREQAAAT
                     PSAERAEAILLRMPVGVVVVNQRHDIQMINVAARRLLGIHSEAIGEDLIRLVQYVPLL
                     PLRNAIDDAFRGEPATLSFSVDALQTATGDALDLELRCYPEPPRSTGSTSATVTLIVF
                     DVSAFESERRSLDGEASLLRGEVQRMTVQVQQLSEANHALVTANDELATINADLRNAN
                     EELLVANEEVQSATEEVETLNEELQATNEELETLNEELQATVEELNTTNDDLQARSIE
                     LQDVAIALEEQRRQVEAERDRIQAILDNMSDAVLVVDAQGQPILSNGAFQEMFGTDGV
                     SFKPEDVAGHPLDESRSLQQRVATGEAFTTEFQVTETDGTRHWYEASAQPIENAAGER
                     LGVVVVRDITDRSLRRLQDEFLALASHELRTPLTSLMGSLQLLQRRISGSVDEQANRQ
                     LEVARRQADHLTTLIHDLVDIVRLQTGRLQLHREPVDLVEVVRDASDAAQGITERQSI
                     EPDLPDSPVIAEVDGNRLAQVLMNLLNNAIIYAPETDLIQIRLRQDGDVAEIRVHDDG
                     PGVPAEDRERIFARFSQIEPAGSERQQSDGLGLGLFIAREIVTAHGGTLAVEETAEPG
                     VTFIVRVPLTTGEPS"
     gene            complement(6107..>6997)
                     /locus_tag="VFV93_06890"
     CDS             complement(6107..>6997)
                     /locus_tag="VFV93_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5165103.1"
                     /translation="QPHLRPWSSVLRVPASDGLVYCKACMAGLEDETRLTAALAEWRP
                     DLMPEVLAIDGRRGWMLTQDAGPMLRTITRAEPPNLAPWLGILPRYAELQIELAPRAG
                     ELLALGAPDHRPGLLADLLTLMLDDDEALRIGPPEGLTDDEVEHLRALVPGVAALSAE
                     LAGYGIADSLDHGDFHDGNVFIGGGRLTLADWGDCRVTHPFFTLGITLRAAAHFAGIA
                     DDAPPILAMRDAYLEPWQAVAPRADLVRAFALAYQLSHISSARNWHRLIVRLTGEQRA
                     QEADTVPYKLRQFLLAAGQL"
BASE COUNT         1178 a         2299 c         2320 g         1200 t
ORIGIN      
        1 gccagcagct ccttctgctc gcgaatgaac agcggcaggt tcggcgagcc gatctcctcc
       61 tcgccctcga tcgtgaactt gaggttgatc ggcggcgcgc cctgggtctg gaccagcgct
      121 tcgatcgcct tgatcggcat gaacaggttg cccttgtcgt cgctggcgcc acgggcgtag
      181 atcatgccgt cgcggatggt cggctcgaag ggtggcgtct cccagaggtc gagtgggtcg
      241 ggcggctgga cgtcgtagtg gccgtagatc agcgccgttg gcttgctcgc gtcgacgatc
      301 cactcgccgt agacgaccgg gttgccgttg gtcggcatga cctcgacctt cgggacgccg
      361 accttgcgca gcgcgtcggc gacccactcg gccgccttgc ggacatcgtc gcgatggtcc
      421 ggcagggcgc tgatgctcgg aatccgcagc agttcgaaca gctcctccag atgctggtcg
      481 tgatgttcat ccagatagct ctcccaggct ggccgttgac tcatgctctc gtttccctcc
      541 catgtccgtg aatcggcttt ggagggatga tacccgtaga ccgccctgcc tgttcagcag
      601 ttggactgac cgacggttgc gaggcacggc agtgacatgc gttgcgtgca tatgcaggca
      661 aactgataga cacaacccgc gctggaatct agagtgaaaa cagcaaaggc cgggtggtca
      721 agtatctctg tgtatcgatg gccccgtaca cagggatatc agcgcctggc tctttgcgtg
      781 ccctgttggc acttcccggc atgccgtatg ctatgagtca cgcaggcatg tcgggtgatg
      841 tggtccatgg attaccggtg cacgtcgccc gggcgtgtcg gttggactcg ctgaagtgat
      901 gacaggtttc cggaggggcc tccgatgtct gaatcatcca tgctgggtgt ggttatcagg
      961 caacgtcgca ttgagttggg ttggacccag gaagaactcg ccgagcggat cagcagcgac
     1021 ggcgagtatg tgcgtcaatc cgagatctcg cggatcgaga gcgggaaggt cgggctgccg
     1081 cgccatgagc gactcgtccg tttggcggac gtgctcgatc tgccgctcgg cgaactgctg
     1141 gctcgttccg gctggaccgg cgcggaccag cactttcacg tcgctgacgg cgacccgacc
     1201 gcaacgaatc acaccgacga actggtgtcg accgtaccga tggagggcat gcccgcgagg
     1261 aagcgtcggc aaccggtcaa cgaaaccgac gccagtgcga tgccggcgct acgccgagcg
     1321 gtgtcaacga tgcgagatga gtcggagcgt ctgaatcgca attgcgagcg gtcgctggag
     1381 ctcgtcgagc aggtgaggcg atcaggtagc attcgcattc gcacgcatga tgactatctc
     1441 gacccctcgg caacctgagt tgacgcgtcc tgttttcgtg cgttgacgcg gggatgatgg
     1501 gaccgagtcg aagctggcac ggtcgcgttc tcgctcaaca gaacggcccg gatcgtgttg
     1561 gcgtgttcct cggccgcttt cgattgctcg gcgaaacgtt ctgccgcgcc atgttgtgat
     1621 tgctcctcgg cgcggtcggc cagccggcgc gttagtgatg ccttctcctc cagcgcgcgc
     1681 atggcggacc agagcgcggc ttccattccc tcgttctggc tggccagcag gctctcgaca
     1741 ctgtaggcat gcccgacccg gcagcggaag cgtagcatct gggcattctc gacctcccag
     1801 aggacaccac cacactccgg gcaggcgaac ggcgagggaa tgccgggccg gtcgtcgttc
     1861 tccagcgcgc tctggtccac ttctgaaatg ctgttctcgt atttcatgcg atcatcaccc
     1921 cgactggaca ttgcggccgc ctccgtctct tcgatgggtt ccgtcacgag ccgggtgatt
     1981 gtcgggccga tcctggcgac ccgcaggcag tagtcgacct tgacgacatg catcgcgctg
     2041 ctcggcatgc ctggaaaggc tgcctcggcg gggtcctgga cgatggcgac gccgccctga
     2101 cgcttcagcg ccagcaggcc ggccgtcccg tcgtcgagcg agccggagag gatgacgccg
     2161 attgcgcgtg ggccgtaaaa ctgcgcggcg ctgcgcatca gcggatcgac cgaggggcga
     2221 tggcggttct cgcgcgggcc atggacgacc cgaacccggc cgtcgtgcag caacaggtgg
     2281 tggtccggcg gcgcgacgta gatccggccg tgccggaccg gttccccatc gtccggatgc
     2341 tcggccggta gttgcccggc ccggctgagg agcgtcggca gggcgctggt cccatccggc
     2401 gggatgtgca ggacgacgaa gacggccgcc gggaggtcgg ctggaaggtg cctggcaatt
     2461 gtcatcagcg cttcgacacc gcctgccgaa gctccgatcg tcacgatgtc gcgcctggcc
     2521 atgctgggac gctcctcgga ccacggtcgc gtgtgcgtat cagtgcccgg ccgtggatgg
     2581 atggccgcga tggcctcgac gcaggccgtc gagtgtgcaa ccatctggtg tacgtcacac
     2641 aatctgtgcc atcgtccgaa ggaaggttca gccgtgtcat cgccggttat cgtacgctga
     2701 gagctgagtt cgacacaatc aggtggagcg cggatgatcc cgaccaccga attgcgtcgt
     2761 catcggccgt gtcgtcggac gcgaaagggg ctggacgatc atggcggagc agcgacaagg
     2821 tgattcgatc gtcgtcatcg gcgcgtcggc cggcgggatc gacgcgctgt cgacgctggt
     2881 gacgaccatc ccggccaatc tgccggcgcc gatcgtggtt gcccagcatc tcgacccgcg
     2941 ccgccccagc cggctcaatg atctgctgtc gaggcgcagc accgttccga tccggacggt
     3001 tgaggatcgc gcggcgctgg agcctggtgt cgtctatctc gtccccgaga acagcgacgt
     3061 cgagattgcc gaccatcagg tgatggtgcg ccgtgacggc cagcgcacgc cgctgccctc
     3121 gattgatctg ctcttcacga cggccgccgc gtcgtacggc gagaacacga tcgccgttat
     3181 cctgaccggc accggcaccg acggcgcggc cggtgcgcgg gatgtcaaag cctccggagg
     3241 gacgatcgtc gtccagaacc cggagaccgc cagcttcccc ggtatgccgc tctcgctggc
     3301 gccgtcgatc gtcgatatcg ttgccaatct ggaatcgatt ggcgcgctga tcggcgacct
     3361 gctctccggc acctacccga tcagccggcc cgacgaggac ctgcacttgc atggcctgct
     3421 cgaacagctt cgcgagcgga gcgggatcga cttcaacgcc tacaaggccc cgacgatcac
     3481 gcgccgcctc cagcgccgga tggccgccac cggcgcatcc agcctgagcg attacatgcg
     3541 ctacctgcag cgcaaccccg acgagtacca gcggctgatc aacagcttcc tgatcaaggt
     3601 gaccgagttc ttccgcgatc cggaactgtt cgagtacctg cgcgagcagg tgttgccggc
     3661 cgtggtcgag gaggggcgcg agcgcggcga gctgcgcctc tggtcggccg gttgcgcgac
     3721 cggcgaggag gcctactcgc tggcgatcct gatggccgaa cagcttggcg acgaactcga
     3781 ttcgttcaac gcccgcatct tcgcgaccga cctggacgct gacgcggtcg gtttcgcccg
     3841 gcgtggcatc tacccatcga cggcgctggc gacgctcccg ccggagatgg tagagcgcta
     3901 tttcacgcgc actgacggcg cttacgaggt ccgtaagagc gttcgctcgc tggtcgtgtt
     3961 cggccagcac gatctcggtc agcgcgctcc gttcccccgg atcgacgtca ccctctgccg
     4021 caatgtgctg atctacttta cggccgatct gcagaagcgc gcgctccagc tggtcgcgtt
     4081 ctcgctgcgc gaccagggct acctgatcct cggcaaggcc gagacggtca gcccgctgcc
     4141 tgagtacttc tcactcgaac agccgcggtt gaaggtctat cgtcgcgtcg gtgagcgggt
     4201 gctgatcccg acggccagga tccgggactc ggtgccgacg ctgccgatcc gcgtgacgcc
     4261 gcctcgcata cacggcgctg acggcgcggc gctgcggctg cgtcgcgagc aggcggcggc
     4321 cacgccgtcg gccgagcgag ccgaggcgat cctgttgcgg atgccggtcg gggtcgttgt
     4381 cgtaaaccag cgccacgaca ttcagatgat caacgtcgcg gcccggcggc tgcttggaat
     4441 ccactccgaa gcgattggcg aggatctgat ccgcctcgtc cagtatgtgc cgctgctgcc
     4501 gttgcgcaac gcgatcgatg acgccttccg tggcgagccg gcgacgctct cgttcagcgt
     4561 tgatgccctc cagaccgcga cgggggacgc gctggatctg gagctgcgct gctatcctga
     4621 gccgccccgc agcaccggca gcaccagcgc gactgtcacg ttgattgtct tcgatgtctc
     4681 ggcattcgag agcgagcgcc ggagccttga tggtgaggcc agcctcctgc gcggagaggt
     4741 ccagcggatg accgtgcagg ttcagcaact gtccgaagcc aaccacgcgc tggtgacggc
     4801 caacgacgaa ctggcgacga tcaacgccga cctccgtaac gccaacgagg agttgctggt
     4861 cgccaacgag gaagtccagt cggcgaccga agaggtcgag acgctcaacg aggaactcca
     4921 ggcgacgaat gaggagctgg aaacgctcaa cgaggagctg caggcaaccg tcgaagaact
     4981 caacacgacc aacgacgatc tccaggcgcg tagcatcgag ctgcaggatg tcgccatcgc
     5041 gctcgaagag cagcggcggc aggtcgaggc cgagcgcgac cgtatccagg cgattctcga
     5101 caacatgagc gacgccgtcc tcgtcgtcga tgcccagggg caaccaatcc tctcgaatgg
     5161 cgccttccag gagatgttcg ggaccgacgg cgtatcgttc aagccggaag atgttgccgg
     5221 ccatccgctg gacgagagcc ggtcgctcca gcaacgggtc gccaccggcg aggcattcac
     5281 gaccgagttc caggtgacgg aaacggacgg cacccgccac tggtacgaag ccagcgcaca
     5341 gccgatcgag aatgcagccg gcgagcggct gggggtcgtc gtcgtccgcg atattaccga
     5401 ccgcagcctg cgtcgcctcc aggatgagtt cctggcgctc gccagccacg agctacgcac
     5461 gccgctgacc tcgctgatgg gcagcctgca actgctgcaa cgccggatca gcggcagtgt
     5521 cgacgagcag gccaaccgtc agcttgaggt cgcccgtcgc caggccgatc atctgacgac
     5581 actgatccac gatctggtcg atatcgtgcg cttgcagact ggccgcttgc aactccaccg
     5641 ggagccggtc gatctggtcg aggtcgttcg cgacgccagc gacgccgccc aggggatcac
     5701 cgagcgccaa tcgatcgagc cggatctgcc ggatagcccg gtcattgctg aagtcgacgg
     5761 caaccggctg gcgcaagtgc tcatgaacct gctcaataac gcgattatct acgcgccaga
     5821 gaccgatctg atccagatca ggctgcgtca ggacggcgat gtcgccgaga ttcgcgtcca
     5881 cgacgacggc cccggcgtcc cggcagaaga tcgagagcgc atcttcgcgc gcttcagtca
     5941 gatcgaacca gccggctcag agcgccagca gagcgacggc ctcgggctcg gcctcttcat
     6001 cgcgcgcgag atcgtcaccg cccacggcgg gacgctcgcg gtcgaagaga cggctgaacc
     6061 cggcgtgacc ttcattgtcc gtgtgccgct gacaaccggc gagccgtcat agctgcccgg
     6121 cagccagcag gaactggcgt agcttgtacg ggacagtatc agcctcctgc gcacgctgct
     6181 cgccggtcag cctgacgatc aatctgtgcc agttgcgcgc gctggagatg tgcgacaact
     6241 ggtatgcgag cgcgaaggcg cgcaccagat cggcgcgtgg agcgacggcc tgccacggct
     6301 ccagataggc gtcgcgcatg gcgaggatcg gcggcgcatc gtcggcgata ccggcgaagt
     6361 gcgcggcagc acgcagcgtg atccccagcg tgaagaacgg atgggtgacg cggcagtcgc
     6421 cccagtcggc cagcgtcagc cggccgccgc cgatgaagac gttgccgtcg tggaagtcgc
     6481 cgtggtcgag cgagtcggcg atcccgtagc cggccagttc ggcagacagg gcggcgacgc
     6541 ccgggacgag cgcgcgcagg tgttcgacct cgtcgtcggt caacccctcg ggcgggccga
     6601 tccggagcgc ctcgtcatcg tccagcatca gcgtcaacag gtccgctagc agccccggcc
     6661 ggtggtcggg cgcgccgagc gccagcagct cgccggcgcg cggcgccagc tcgatctgca
     6721 attccgcgta gcgcggcagg atccccagcc acggcgccag gttcggcggc tcggcccgcg
     6781 tgatcgtccg tagcatcggt ccggcgtcct gcgtcagcat ccagccccgc ctgccgtcga
     6841 tcgccagcac ctccggcatc aggtccggcc gccactccgc cagcgccgcc gtcagccgcg
     6901 tctcgtcctc caacccggcc atgcacgcct tgcagtagac cagcccgtcg ctggccggca
     6961 cccgcagcac gctcgaccac ggccgcaggt gcggctg
//
LOCUS       DASUGJ010000312         7047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1575769, whole genome shotgun sequence.
ACCESSION   DASUGJ010000312 DASUGJ010000000
VERSION     DASUGJ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575769"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..495
                     /locus_tag="VFV93_06895"
     CDS             208..495
                     /locus_tag="VFV93_06895"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX5165104.1"
                     /translation="MEDPLGDTNQRDFQAVGAASGLGCTVVVSLLLCIGGGVLLDNWL
                     GTSPVFVLIGVALGLAAAGYALYELAVLGQPDRGRIKLKRSRVKNGPDKPG"
     gene            527..1450
                     /gene="atpB"
                     /locus_tag="VFV93_06900"
     CDS             527..1450
                     /gene="atpB"
                     /locus_tag="VFV93_06900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5165105.1"
                     /translation="MEIHVSVKPETLWSVGPLDITNSFFTMIIVMVVLIVAGAIIARS
                     ASIDRPGKVQSAAEVVVEFLQNLVYSTAGRRAGQRIFPLVGGLFFFIIVSNYAGLLPG
                     VGTIGLWDEHEEEATSEESRVASAAQAGGPEVVYAAQEEEAEHDEKVLSPFLRSPSAD
                     LNMTLAMALLTFVIVQFAGVAAHGIGGRIKHMADPWWIFPIELISEVSRIISLSFRLF
                     GNIFAGEVLVAVMIAMSRAMLVKFYVFPLIALPTVFLFLEVLFGFIQALVFALLTLIY
                     IALAAAGHDDHEEEHAHGDLQTAPAAGSAGD"
     gene            1617..1859
                     /gene="atpE"
                     /locus_tag="VFV93_06905"
     CDS             1617..1859
                     /gene="atpE"
                     /locus_tag="VFV93_06905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX5165106.1"
                     /translation="MTGPEGLDAAKAIGAALAIGLGAIGPGLGIGLAVKGAMEALGRN
                     PEAEGAIRTTMIIGAALAEAVAIYAFVIAIIVAFVV"
     gene            2075..2587
                     /gene="atpF"
                     /locus_tag="VFV93_06910"
     CDS             2075..2587
                     /gene="atpF"
                     /locus_tag="VFV93_06910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX5165107.1"
                     /translation="MGDLGINGWSFIVQLLAFILFIVLFWRYALGPISRTLEARQDRI
                     KESIEAAQRMQQELAATQARNEEILREAQVESQAIIARAREVSEQNIARSREQAQVQA
                     DDMIAKAREAIAAESAQARTELRREVADLAVMAASKIVRANLDRQAQARLIEETLAEA
                     TSGSTTISPN"
     gene            2606..3139
                     /locus_tag="VFV93_06915"
     CDS             2606..3139
                     /locus_tag="VFV93_06915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX5165108.1"
                     /translation="MAARGVAKRYAQATFDIARESGTEQQWLEDLTTLANAAGDPSLG
                     DYFTSPNIAEQAKLDALGQILPAERQQLARNLASMLVQRRRFDVLPELLEVYRDLLLQ
                     AQGVAIAEVTTAVELSPAEQQAVQAQLAQAVGQQIEMRMRVDPSLVGGLVARIGDWLI
                     DGSVESQLRNLRASLAR"
     gene            3306..4835
                     /gene="atpA"
                     /locus_tag="VFV93_06920"
     CDS             3306..4835
                     /gene="atpA"
                     /locus_tag="VFV93_06920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5165109.1"
                     /translation="MAVRATEIRDIIKNQIQSFDAVATQTNVGNVIEVGDGIATVYGL
                     SDVMANELVQFSNGIMGMAFNLQEDSVGVIIFGDFTGIEEGDEVRSTGRIAEVPVGRA
                     LVGRVVNALGEPVDGKGPITTDSFRAVERIAPGVIERQNVDTPLQTGIKAIDSMIPIG
                     RGQRELIIGDRQTGKSAIAIDTIINQRESGVICIYVAIGQKKQQVAQLVATLEKYGAM
                     DHTIVVVASAAEPAPMQFIAPYAGTAMGEEIMETGGDALIIYDDLSKHAWAYRQVSLL
                     MRRPPGREAYPGDVFYLHSRLLERSARLSDERGGGSLTSLPIIETQANDVSAYIPTNV
                     ISITDGQIFLESDLFYAGVRPALNVGISVSRVGGAAQVRAMRQVAGSLRLDLAQFRSL
                     AAFAQFASDLDPATKRQIDRGLRLTEILKQPQYQPMPVEQQVAVIWAATNGFVDEIPA
                     DRVRSWENQYVEYLTTAGSDLLATIKEKRALDDDSTAALRTLTEQFARGLSVDQLAAV
                     V"
     gene            4937..5812
                     /locus_tag="VFV93_06925"
     CDS             4937..5812
                     /locus_tag="VFV93_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX5165110.1"
                     /translation="MATPREIRRRIRSVRNTSQITRAMEMVSAAKMRRAQERVRAARP
                     YAERIRLMIAGLSALGASVDQSAFPLLQQRPVNRVGVVLVTADRGLAGAFNTNVIRRA
                     VRFIREEAGHPAELITVGRKGRDFFARTTQEIEAEFTALGDAPSMDTLRPIIDISIQD
                     FIDGKVDAVYLIYTKFVNTLTQQPEVLQILPITAPEDASAEEQVQDFIFEPDPVTVLQ
                     ALLPRYIETQVYQALLESVASEHSARMVAMRAASDNANEFINELTLTLNKARQAQITR
                     EVSEIAAGAAALGGR"
     gene            5882..>7047
                     /gene="atpD"
                     /locus_tag="VFV93_06930"
     CDS             5882..>7047
                     /gene="atpD"
                     /locus_tag="VFV93_06930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5165111.1"
                     /translation="MATTTGGAVGEVVQIQGAVVDVEFPPDQLPEIYNALEIPMDNGS
                     TLTLEVQQHLGNDWIRAVAMSSTDGLRRGAKATDIGAPIQVPVGPATLGRIFNVVGEP
                     VDGNPTVNAEQTYGIHRPAPEFDEQSTTIEIFETGLKVVDLIAPFTKGGKTGIFGGAG
                     VGKTVVIMELIRNIAAEHGGYSVFCGVGERTREGTQLYGEMHESGVIDKTAMVFGQMN
                     EPPGARLRVALSGLTMAEYFRDEGRDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQ
                     PTLATEMGQLQERITSTKRGSITSVQAIYVPADDYTDPAPATAFAHLDSTVSLERSIA
                     EQGLYPAVDPLSSTSRALDPLIVGEEHYRTGREVQRILQRYRDLQDIIAILGIE"
BASE COUNT         1235 a         2269 c         2327 g         1216 t
ORIGIN      
        1 gtagtgagtg ctgaaaattc cgcgaaaacc cctccccaac ccctcccctg gaaagggagg
       61 ggctttgcgc cgcacggaat ctgacgcccc tcgccgcgca ggggaggggt tggggagggg
      121 ttttcgcgga attttcagca ctcactaccc agcactgcca aaagattgtg atacaatgca
      181 caacagcgtg agaggcgggg gcctggtatg gaagacccgc tgggggacac gaatcagcgc
      241 gattttcagg cggtaggtgc cgcgtcgggg cttggctgca cggttgtggt cagcctcttg
      301 ctgtgtatag gcggcggtgt cctgctggat aactggctgg ggacatcgcc ggtctttgta
      361 ctgatcggcg tggcgctggg acttgcggca gccgggtatg cgctctacga gctggcggtc
      421 ctgggccagc cggatcgcgg ccgaatcaag ctgaagcgct cgcgcgtgaa gaatggtccg
      481 gacaagccgg gctgacgggc agagggaacg gagttgggaa aactagatgg agatccacgt
      541 ttcagtcaag cctgagacgc tgtggtcggt agggccactc gatatcacca actccttttt
      601 caccatgatc atcgtgatgg tcgtcctgat cgtcgccggc gccatcatcg cccgcagtgc
      661 ttcgatcgac cgccccggca aggtgcagtc ggcagccgaa gtcgttgtcg aattcctgca
      721 aaatcttgtc tactcaaccg ccggccggcg tgcaggccag cgcattttcc cgctggttgg
      781 cggtctgttc ttcttcatca ttgtttcaaa ctacgccggg ctgttgcccg gtgtcggcac
      841 aatcggcctc tgggacgagc acgaggaaga agcgaccagc gaagaatcgc gcgtcgcctc
      901 agctgcccag gccggcggcc cggaagtcgt ctacgccgct caggaggagg aagccgagca
      961 cgacgagaag gtgctgtctc cgttcctccg ctcgcctagc gccgacctca acatgacgct
     1021 ggcgatggcg ctgctgacct tcgtgatcgt ccagttcgcc ggtgttgccg cccacggtat
     1081 cggtggccgc atcaagcaca tggccgatcc gtggtggatc ttcccgatcg agctcatctc
     1141 cgaagtctcg cgcatcatct cgctctcgtt ccgtctcttc ggcaacatct tcgccggcga
     1201 ggtgctggtg gcggtcatga tcgccatgtc acgcgcgatg ctggtcaagt tctatgtctt
     1261 cccgctgatc gccttgccga ctgtcttcct cttcctggaa gtgctgttcg gcttcatcca
     1321 ggcgctcgtc ttcgcgctgc tgacgctgat ctacatcgca ctcgcggcag ccggacatga
     1381 tgaccacgag gaagaacacg cgcacggcga cctgcagacg gcgccggcgg caggcagcgc
     1441 cggcgattag gccggcgttg cccctgattc ttgcgatcgc ggggcggtat tgcccccgat
     1501 gggtatatgt gcagcatggg cgcttcgtgg ggagtgtccg aggcaaagtg ggatgttggt
     1561 cgcggccatc tgggaccgcc tgaccagcga agggagacga tagcgtgttt ggtggcatga
     1621 ctggccctga gggtctcgac gccgcgaagg caatcggcgc tgcgctggcc attggcctcg
     1681 gcgcgatcgg tccgggcctg ggtattggcc tggcggtcaa gggcgcgatg gaggctctcg
     1741 gccgcaatcc ggaggctgaa ggcgcgatcc gcaccacgat gatcatcggt gcggcgctgg
     1801 ccgaagccgt cgcgatttat gcgttcgtta tcgcgatcat cgtcgccttc gtcgtttagt
     1861 tccgggcgct atcgatcgtc ggacgcggtt gatcgtcatg cgagccgtgc tgctgacctg
     1921 gctgtgatgg gacgggcgac aatccgtccc gtcgggccgt ccgggtcgag ccggtgatcc
     1981 agccggacgc ggcgttgaga cagcgttgca ctccgcacgt ggagtgcgga ttgaaacaac
     2041 cgatcaagca cacggaagca gggcgaataa gcagatgggt gatctaggaa tcaacggctg
     2101 gagctttatc gtccagctcc ttgccttcat cctcttcatc gtactcttct ggaggtacgc
     2161 cctcgggccg atcagccgga cgctcgaagc ccggcaggat cgcatcaagg agagcatcga
     2221 ggccgcgcag cggatgcagc aggagctggc cgcgacgcag gcacgcaacg aggagatcct
     2281 gcgcgaggcg caggtcgagt cgcaggcgat catcgcccgc gcccgcgagg tcagcgagca
     2341 gaacatcgcc cgctcgcgcg agcaggcaca ggtgcaggcc gatgacatga tcgccaaggc
     2401 ccgcgaggcg attgccgccg aatcggccca ggcgcggacc gagctgcgcc gcgaggtcgc
     2461 cgatctggct gtgatggctg cctcgaagat cgtgcgcgcc aacctcgacc gccaggctca
     2521 ggcacggctg atcgaggaga cgctggccga ggcaacttcg ggcagcacga cgatctcgcc
     2581 gaactaggcg gggaggggag agcgagtggc tgcacgaggc gtcgcaaagc gatatgccca
     2641 ggcgaccttt gacatcgcgc gcgagtccgg caccgagcag caatggctcg aagacctgac
     2701 gacgctggcc aacgccgccg gcgatccgtc gctcggcgac tacttcacca gtccgaacat
     2761 tgccgagcag gccaagctcg acgcgctcgg ccagattttg ccggccgagc gccagcagct
     2821 tgcccgcaac ctcgcctcga tgctggttca gcggcggcgt tttgatgtgc ttccagaact
     2881 gctggaggtc tatcgcgacc tgttgcttca ggcacagggc gtcgcgatcg ccgaggtgac
     2941 gaccgctgtc gagttgtcgc cggccgagca gcaggctgtc caggcgcaac tcgcgcaggc
     3001 ggtcgggcaa cagatcgaga tgcgcatgcg ggtcgatccg tcgctggttg gcgggttggt
     3061 ggcgcgaatt ggtgattggc ttattgacgg cagcgtcgag tcgcagttgc gtaacctgcg
     3121 cgcgtcgctg gcccgctaat gtcccggtag ggcgtggggt tgggggtgtc ccccaacgct
     3181 caaacccgat ccggacacgt taaagtggcc atcgcggacg agggtgtccg cattggagat
     3241 gcacgaacag gtacgttttc agcaaccagg gacgcgccgc gaggcgcctg gcatgggagg
     3301 ccgacatggc tgtacgcgcg accgagattc gcgacataat caagaaccag atccaatcct
     3361 tcgacgccgt tgcgacacag accaacgtcg gcaacgtcat cgaggtcggc gacggtatcg
     3421 caaccgtcta cggcctgagc gacgtgatgg ccaacgaact ggtccagttc tcaaacggca
     3481 tcatggggat ggccttcaac ctccaggagg actcggtcgg cgtcatcatc ttcggtgact
     3541 tcaccggcat cgaggaaggc gacgaggtcc gctcaaccgg tcgcatcgcc gaggtgccgg
     3601 tcggccgcgc gctggtgggc cgtgtcgtca acgcgcttgg cgagccggtc gacggtaagg
     3661 ggccgatcac aaccgacagc ttccgcgctg tcgagcggat cgccccaggc gtcatcgagc
     3721 gccagaacgt cgacactccg ctccagaccg gcatcaaggc gatcgactcg atgattccga
     3781 tcggccgcgg tcagcgcgag ctgatcatcg gcgaccggca gaccggtaag tcggccatcg
     3841 cgatcgacac gatcatcaac cagcgcgaaa gcggcgtcat ctgcatctac gtcgcgatcg
     3901 gccagaagaa gcagcaggtc gcccaactgg tcgcgacgct ggagaagtac ggcgcgatgg
     3961 atcacacgat cgtcgtcgtc gcctcggctg ccgagccggc cccgatgcag ttcatcgccc
     4021 cctacgccgg cacggccatg ggcgaggaga tcatggagac gggcggcgac gccctcatca
     4081 tctacgacga tctctccaag cacgcctggg cctaccgcca ggtttcgctg ctgatgcgcc
     4141 gcccgccggg ccgcgaggcg tatccgggcg acgtcttcta cctgcactcg cggctgctgg
     4201 agcgctcggc gcgcctgtcg gacgagcgcg gcggcggctc gctgacctct ctgccgatca
     4261 tcgagacgca ggccaacgac gtctcggcct acatcccgac caacgtcatc tcgatcaccg
     4321 acggccagat cttcctggag agcgacctgt tctacgccgg tgtccggccg gccctgaacg
     4381 tcggtatctc cgtctcgcgc gtcggtggcg cggcccaggt gcgcgccatg cggcaggtgg
     4441 ccggttcgct ccggctcgac ctggcccagt tccgctcgct ggccgcgttc gcgcagttcg
     4501 cgtccgacct cgacccggcc accaagcgcc agatcgaccg cggtctgcgc ctgaccgaga
     4561 ttctcaagca gccgcagtac cagccgatgc cggtcgagca gcaggtcgcc gtcatctggg
     4621 cggcaaccaa tggcttcgtc gacgagattc cggccgatcg cgttcgctcg tgggagaacc
     4681 agtacgtcga atacctcacg acggccggga gcgacctgct ggcgacgatc aaggagaagc
     4741 gggcgctcga cgacgactcg acggccgcgc tgcgcacgct gactgagcaa ttcgcgcgcg
     4801 gcctgtcggt cgatcagctg gcagcggtcg tttaggaaca tcgagttgcc tgcgctcggt
     4861 ctggtgttgt agctcagatg accagaccac gctgagcgcg gagcggccta cggagagcta
     4921 gaggaggcac caggcggtgg cgacaccacg agagatcagg cggcgcatcc gctcggttcg
     4981 gaatacatcg cagatcacgc gcgcgatgga gatggtctcg gcggccaaga tgcggcgcgc
     5041 tcaggagcgc gtgcgtgccg cccggcccta cgccgagcgt atccggctga tgatcgccgg
     5101 tctctcggcg ctgggtgcgt cagtcgatca gtcggcgttc ccgctcctgc agcagcgacc
     5161 agtcaaccgg gtcggtgtcg tgctggtgac ggcggaccgt ggcctggccg gcgcgttcaa
     5221 caccaacgtc atccgtcgcg cggttcgctt catccgcgaa gaggccggcc acccggccga
     5281 gttgatcacg gtcggccgca agggtcgcga cttcttcgcc cggacgacgc aggagatcga
     5341 ggctgagttc acggccctcg gtgacgcacc gtcgatggac acgctccggc cgatcatcga
     5401 catctccatt caggatttca tcgacggtaa ggtcgacgcc gtctacctga tctacaccaa
     5461 gtttgttaac acgctgacgc agcagcctga ggtcttgcag atcctgccga tcaccgcgcc
     5521 ggaagacgcg agtgccgagg agcaggtcca ggacttcatc ttcgagccgg acccggtcac
     5581 cgttctccag gcgctgctgc cgcgctacat cgagacgcag gtctaccagg cgttgctcga
     5641 atcggtcgcc tccgagcact cggcgcggat ggtcgcgatg cgggccgcca gcgataacgc
     5701 caacgagttc atcaacgagc tgacgctgac gctcaacaag gcgcgtcagg cccagatcac
     5761 gcgcgaggtg tccgagatcg cggccggcgc ggctgccctc ggtggacgat agagacggca
     5821 catcgttcca ggcgcgctca tggcgcggga gacatgcagg gacaggaagt aggagacaac
     5881 cgtggcgaca acaacaggtg gagccgtcgg cgaggtcgtg cagattcagg gcgccgtcgt
     5941 tgacgtcgaa ttcccgcccg accagctgcc ggagatctac aatgcgctcg agattccgat
     6001 ggacaacggc tcgacgctga cgcttgaggt ccagcagcac ctcggcaatg actggattcg
     6061 cgcggtcgcg atgtcctcga ccgacggact gcgccgtggc gcgaaggcga ccgacatcgg
     6121 cgcgccgatt caggtgccgg ttggcccggc gacgctgggc cgcatcttca acgtcgttgg
     6181 cgagccggtc gacggcaacc cgaccgtcaa cgccgagcag acctacggca tccatcgccc
     6241 ggcgccggag ttcgacgagc agtcaacgac gatcgagatc ttcgagaccg gtctgaaggt
     6301 cgttgacctg atcgcgccgt tcacgaaggg cggcaagacc ggcatcttcg gcggcgccgg
     6361 tgtcggcaag acggtcgtca tcatggagct gatccgcaac atcgccgccg agcacggtgg
     6421 ctactccgtc ttctgcggcg tcggcgagcg cacccgcgag ggcacccagc tctacggcga
     6481 gatgcacgag tcgggcgtta tcgacaagac ggcgatggtc ttcggccaga tgaacgagcc
     6541 gccgggcgcc cgcctgcgcg tcgccctctc cggcctgacg atggccgagt acttccgcga
     6601 cgagggccgc gacgtgctgc tcttcatcga caacatcttc cgcttcgtcc aagccggttc
     6661 cgaagtgtcg gcgctcctcg gccggatgcc gagcgccgtc ggttaccagc cgacgctggc
     6721 gaccgagatg ggccagctcc aggagcggat cacctcgacc aagcgcggct cgatcacgtc
     6781 cgtccaggcc atctacgtcc cggccgacga ctacaccgac ccggctccgg cgacggcctt
     6841 cgcccacctc gactcgaccg tctcgcttga gcgctcgatc gccgagcagg gcctctaccc
     6901 ggctgtcgac ccgctctcct cgacctcccg cgccctcgac ccgctgattg ttggcgaaga
     6961 gcattaccgc accggccgtg aggtgcagcg catcctccag cgctaccgcg acttgcagga
     7021 catcatcgcg attcttggta ttgaaga
//
LOCUS       DASUGJ010000313        15594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_46417, whole genome shotgun sequence.
ACCESSION   DASUGJ010000313 DASUGJ010000000
VERSION     DASUGJ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15594
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46417"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFV93_06935"
     CDS             complement(<1..248)
                     /locus_tag="VFV93_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165112.1"
                     /translation="MSSGGTIDRMLDSIPGYAGYRDKERRRDSDRVIRENLAREYGQL
                     ADRLGRIATSLADERKILLIPTVDKPFKRLTSFIDRVRT"
     gene            complement(288..818)
                     /locus_tag="VFV93_06940"
     CDS             complement(288..818)
                     /locus_tag="VFV93_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165113.1"
                     /translation="MTLTGRTAGIIMVVAGVLVGAVAVAWLASSLADDESGLRLSGAV
                     FGGALLFIVLVLPLLAGGIFLYIRGGAEARQFANAERQRKMLGIVESAGEISIADLAL
                     QIQGTRDSVRSDLYDLVSKGLFAGYVDWDRGILFARQASELRGKENCPNCGGQLSLAG
                     KGLVRCPYCGAEIFLP"
     gene            complement(815..1888)
                     /locus_tag="VFV93_06945"
     CDS             complement(815..1888)
                     /locus_tag="VFV93_06945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5165114.1"
                     /translation="MGARKPERILAIKLADLGDLLLCEPAFRSLRAAFPEARIDVLTT
                     PSSAALLPLIGHGLRPIEFPKQLFDSLAGLTRPSAQVRAVRLALTLRSARYDLVVLLH
                     HLTTAFGARKFAALAQATGCRSVVGLDNGRGTFLSVRVIDEGFGARHEATYMRDVAVA
                     AGGISVDATPCIAVPQARPPAGTPSTPYATIFPVTGQYSRAREWSVQRFGAVATRLAA
                     QGIIPLVVGAQDASEAAATIRATEPSTIDLTGRTSLEELAVVLAGAQVAVGGDTFIGH
                     LAAAVGTPIVSIFGPSNARAWRPIGRSGTVEIVCAELPCQPCIYTGFRLGRPAGCPDR
                     TCLKLVTTNNVASAVEYVLGGIG"
     gene            complement(1875..2981)
                     /locus_tag="VFV93_06950"
     CDS             complement(1875..2981)
                     /locus_tag="VFV93_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX5165115.1"
                     /translation="MHADEIAVDASRAGVAARTGTEWYSFELIRAMIAVEDRPPLRLY
                     HRGAPPPELDGPAVSHQHIQQPRLWTHLGLSRAMRHDRPAALYVPSHVLPLVHPAVSV
                     VTVHDLGYLAEPDAHPASTRRMLDLTTRWNARTAREIIAISGQTRDDLVRHYRVDPAK
                     ISVIHSGVDHQRFRPLDVDTVQAVISRLGINQPYLFFLSTVQPRKNVERLVEAFESLE
                     PGLTLVIAGSSGWLSEPIEARIAGSSASDRIRRLGVVADADVPALYNGAEAFVLPSLF
                     EGFGMGVLEAMACGCPVVTSSRSSLPEVAGGAAILIDPFDIRSIRDGIVAAIKPQRRA
                     ELVAAGIERARNFTWQQTATATLDVIRKAIDGRS"
     gene            complement(2971..4104)
                     /locus_tag="VFV93_06955"
     CDS             complement(2971..4104)
                     /locus_tag="VFV93_06955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5165116.1"
                     /translation="MQRLTAAVRDRLLPAAAHLRPSRWEPTEDTVLIIQPDHLGDILL
                     SQLAVRHLRLRFPDSRLVAVVGPWSREIAEMSWPVDEILTVEFPGFTRERSMSMTAPY
                     RQLTAESQRLRALHPRSAYVLRPDGWWAAWLASLIAPEVVTAADPRTSAFATRTAVVH
                     DGDHASVRAFRIASNGDDARVPTVLSDPLHLTPSPDAVALATRLLRSHRVNGGYVVIH
                     PGSGASVKEWPAQRWRHVASALADAGYSIVLTGSMPERVITDGIARDGTGAVNLAGQT
                     TVPVLTEILRGASLVLGPDCGPLHLAVAADTPSVHLFGPSDPVRYGPWGDPVRHRVIS
                     AGWRCPRCGDLSAGRGSGCGCMLAIQPNEVAAVARALLANHAR"
     gene            complement(4092..5105)
                     /locus_tag="VFV93_06960"
     CDS             complement(4092..5105)
                     /locus_tag="VFV93_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5165117.1"
                     /translation="MTPERVAILKPCCLGDCVMALPAIDTVLAAWPASEVDVFVGAHS
                     RSAFAFRSRLHLRRVRDSLDVPSALQLSRVLRQGQYDLVVVLDRSRLLNGASRLARAR
                     KSVAIHTRAPELRHETEVYLDVMRQLGISTPVTTPSITPSVVARQAAKGLLPDQSAPL
                     SILHPGGARNPGANMLEKRWPADRFTQLASELKRDGISVLLSGGPDDVALASQIARDA
                     GLEDWSILAGRTDIGTLAAILEQAAVYVGPDTGVSHIAAAVGTPTVAIFGPTNPRRYR
                     PLGPDVHVLAPAASWSIVDRDLRRRTDEPGPISTNLVTFDPVLRAAREAIDRHQDRRR
                     CND"
     gene            complement(5117..5557)
                     /gene="fabZ"
                     /locus_tag="VFV93_06965"
     CDS             complement(5117..5557)
                     /gene="fabZ"
                     /locus_tag="VFV93_06965"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX5165118.1"
                     /translation="MLDAQQIQAIIPHRYPFLLVDRIVEIEYGVAAVGIKNVTANEPF
                     FQGHFPDYPVMPGVLIVEALAQVGAVAILGMEEFKGKMPFFAGIDGVRFKRQVKPGDT
                     LRLEVTLGKMRRGVGMGTATATVDGELAVRGELMFAVADISTPS"
     gene            5625..6668
                     /locus_tag="VFV93_06970"
     CDS             5625..6668
                     /locus_tag="VFV93_06970"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX5165119.1"
                     /translation="MSINAAPESAGGERTVVIGAGAWGTTLANLIASTGRDVTLVAHS
                     QAHAEQVRLARENERYLPAIRLSDRLLVTDDPTSVVSGASMVALVVPSQQMRASASKV
                     APFLSPEAVLVSCAKGLELGTLLRMTEVVADASGVATNRICALSGPNLAREIALGLPA
                     SAVVAGSDQRVSEMAQLALGAPSFRLYTSADVVGVELGGALKNIVALGAGVADGLQLG
                     HNAKAAFITRGLAEMIRLGVAAGANPLTFGGLSGLGDLIATCESPLSRNRSFGERLGR
                     GMSIEEAAAGTPHVVEGLTTARAALTLAEHHRVEMPIAATVVSVLDGRMGVRDAIGSL
                     MSRGARPELDAFR"
     gene            complement(6665..7951)
                     /locus_tag="VFV93_06975"
     CDS             complement(6665..7951)
                     /locus_tag="VFV93_06975"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX5165120.1"
                     /translation="MPVSIVLGGQWGDEGKGKIIDALANSADVVIRANGGANAGHTVV
                     TDKGVFKFHLIPSGILHPDCVCIIGAGVALNPKTLLEELDRLDERGIDSSNLRISSRC
                     HLIMPYHAVIDQLEESSRGVDMIGTTQTGNGPCYADKAARRGIRLADITDPEHLNRML
                     APVLAEKNAMLQAVYKHEPFDLNQIAEEYAAYGERLAPYLAEVESDVQDAIENGANVI
                     VEGAQAAMLDIDYGTYPFVTSSSPTAAGVCQGAGIAPTQVGRVIGVYKAYLTRVGAGG
                     FPTELLDATGDHLRERGVEFGTTTGRPRRTGWFDAVQARFTARLNGISDIALTKFDVL
                     DGMESVKICTSYELGGEPIAAPPARISDYERVRPVYEELPGWDEDTSDAAALSDLPAN
                     ARAYVQRIEELIGVPIRFVGVGPHRRQLLVSPIAAG"
     gene            complement(8032..9063)
                     /locus_tag="VFV93_06980"
     CDS             complement(8032..9063)
                     /locus_tag="VFV93_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX5165121.1"
                     /translation="MQFQDYYSVLGVSRSASEGDIKKAYREKARKLHPDVNKNADAEE
                     QFKAINEAHQVLSDPEKRARYDRFGSDWERYQSAADSGGASDFSQWFSGQAAGNPNVR
                     FDYRTTGGEGFSDFFETLFGSGGRSSRTQRRAPRRGDDHEYPIDVSLHDAFTGTTRAF
                     EMQTPEVCPDCGGTGIIQGSICFTCEGSGSTTKRSRIEVTIPAGIRDGQRVRVAGKGS
                     PGRDGGPAGDVYLKVRIKPDPAFTLEGSDLKTEVTVPLYTAILGGEALVRTMTGRVAL
                     TIPPETQNGRIFRLRKLGWPAAVGSTERGDLLVRVNVVLPTDLSERERELFEQLQDAR
                     QGAGVTMNA"
     gene            complement(9144..9716)
                     /locus_tag="VFV93_06985"
     CDS             complement(9144..9716)
                     /locus_tag="VFV93_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX5165122.1"
                     /translation="MFELLTGLGLAMPAGLNAWIPLLAVALADRYTGLIKLAEPYDML
                     SSPIAIAIIAVFLGIELLADKIPIVDHVNDLIQSAIRPSAGAILLMASTDTVDSINPI
                     IAMILGLLVAGGIHTTKTSFRPVVTATTGGIGNPIVSAVEDGTAIIVTVIALIAPILI
                     AIILILLLALIFTLFRRRRRRRQLIEAGSA"
     gene            complement(9756..10397)
                     /locus_tag="VFV93_06990"
     CDS             complement(9756..10397)
                     /locus_tag="VFV93_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HEX5165123.1"
                     /translation="MDIGRAYTFITSDPKWIAKILIGAAVSLVPLLNLALYGYALDVL
                     KRVYQGNELPLPEWDDFGDYFIRGLLVSIGIVIWELPLFLLACPIVALAIIDNSGVAV
                     LLGLACLIPLGIVYAAITCQIVAARYALSGEFSSMFEFGAVFAEARRGAGPLLLCALI
                     YAAASVAGMVAGVVTCFVGLFIVGPWLTLAQAHLLGQAYRGVRNLPPTTATAF"
     gene            complement(10492..11922)
                     /locus_tag="VFV93_06995"
     CDS             complement(10492..11922)
                     /locus_tag="VFV93_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165124.1"
                     /translation="MTRSDRRWLFAVIAIVAVAQIALAIRVLDRLDPLTGDEPFYVMT
                     ALSIMNDRDLDESNNYADRDYDEFYPPEPLPAGWDGWPAFPRTLPPHPATSDRNGLHT
                     KHGLGLSFLIATPYEIAGRPGAVAVVLICSLFVAANMFLLAHDVNASPAWAAAIAIML
                     AVSMPLAPYSSLLFPEIPAALLLIYAIRRLATPSNALYVWVLTGMAIGFLPWLHQRFA
                     PTAVVLTGIVLYRFWKSREYSGAIAALTPVAVGGLSLVGYNLWLYGSPFQSTADHAGF
                     NGPEGTLNATFGLLLDAQWGLLIAAPLYAVVLAGIPFWLKSSPTARMAALAIVPYLVV
                     VATYRVWWGEWGPPARYLVPVAPLAAGVLATLVVHVSTPVRAAIVGLWCAGMVLVAAG
                     AINPQRFYHQPDGINNLYMVLDGWFGTDIAGHLIAFQPYAISPLDQRVVASLMLCTII
                     ALMIVVAWPVDRQRAAQPTEEISAGD"
     gene            complement(11919..12302)
                     /locus_tag="VFV93_07000"
     CDS             complement(11919..12302)
                     /locus_tag="VFV93_07000"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX5165125.1"
                     /translation="MKQLIVRIIPAVAWMAFIFFMSSQEQFPSTLGVSIMLLTVVGHM
                     VLYGVLGLLLLFAFAGTARPSRTTMAAAVLGAALYGVTDEFHQSFVPGRDASVFDLFV
                     NTVGATLAVISSTFARSTLTSASSR"
     gene            complement(12299..13543)
                     /locus_tag="VFV93_07005"
     CDS             complement(12299..13543)
                     /locus_tag="VFV93_07005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5165126.1"
                     /translation="MRMCPARFASDVATARARPVPSPAMQRRAESVDIVMAGTFAAWR
                     LGTLQARALPLARELTSRGIRSAIVTVPWDMPGEAGVVDVIDGVSLTNTSATGVTSFP
                     AAVLQQARAIRALRPQAVHVFKPKGYGGLAGKLLSGSTPLLVDSDDWEGDGGWNQAGN
                     YSVLQRRLFDWQERNLLRDAVAVTAASQLLACRAIALRARTPHQVTWVPNGLADRWAK
                     QLFEARKPMPVAVGLPPTIVLYSRFAEFAHDWLPRYLASLSAFLSADTSVVVRIIGGE
                     PARDIGKANLVLDQMGYVAWNRIPELLGSSDIAIYPYADSLIARSKNSVKLLELMAAG
                     CAVVASEVGDVPAVAGGCAVLLHRSDPEEFAESTVRLLRDPDRISRLSEAARARVLQQ
                     FSVQASADRLVTAYRQAGVTAS"
     gene            complement(13581..14222)
                     /gene="lexA"
                     /locus_tag="VFV93_07010"
     CDS             complement(13581..14222)
                     /gene="lexA"
                     /locus_tag="VFV93_07010"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX5165127.1"
                     /translation="MKQLSRRQQDILGYIESFSDEHSYPPTIREIQEGLAISSTSVVD
                     YNLNVLEQRNLIRRNRNISRGIELVGRPLARRGIISIPVVGRIAAGEPIPVPDDLATQ
                     EYLDTIEISSDVLGPRTEGLFALRVKGHSMVDALINDGDVVVLRHQVVCENGDTVAVW
                     LKGEKETTLKRFYHDNGQVRLQPANVTMDPIYTTADNVEIQGKLVTVIRPVIQ"
     gene            14454..15029
                     /locus_tag="VFV93_07015"
     CDS             14454..15029
                     /locus_tag="VFV93_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX5165128.1"
                     /translation="MNVLDFGIVLVFAILIMVGFFGGVGRVTAGFFAVYFASIVSAAF
                     YEGLGDIFIDRVSDMREATAYLLSFMLLFVSLSGAFFWAIGATIKAVELRRGRFAIMD
                     NVGGATMALVVGAVAVAMTLSVSVIILGAFNQSAVVNGDENLGTLGKQIRGSELVPMF
                     LKLQEPILVVYRPWFPEGLPPILKTPPQSVT"
     gene            15047..>15594
                     /locus_tag="VFV93_07020"
     CDS             15047..>15594
                     /locus_tag="VFV93_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX5165129.1"
                     /translation="MATDILTTLEFDRVRENLQRHCQFSLSVERAGELGPSSDPTRVR
                     YLLDVTGEAYMLIEAYPAFGVRGARDIRRHLQRAELGGMLQPTDLLEALDMITASRTT
                     RRTFLRMEDAVERFPSIDEFVSFIVELPDLEAELQRSISERGEVLDTASEALRKIRQE
                     LRSAHARLIERVTRYIQGSQYSA"
BASE COUNT         2934 a         4783 c         5084 g         2793 t
ORIGIN      
        1 gtcctgaccc ggtcgatgaa tgaggtcagg cgcttgaacg gcttgtcgac tgtcgggatc
       61 agcagaatct tgcgctcgtc cgccagcgac gtggcgattc ggccgagtcg gtccgccagt
      121 tgaccgtact cgcgcgccag attctcgcga atcacgcggt cggagtcgcg tcggcgctcc
      181 ttgtcgcgat agccggcata gccggggatg ctgtcgagca ttctgtcgat cgttccgccc
      241 gaggacatga ctccacctcc aaccagacgt tcgtgacggc gcaccactca gggcaggaag
      301 atctcggcac cgcaatatgg gcagcggacg aggcccttgc cagccagcga gagttgccca
      361 ccgcagttcg ggcagttctc tttccctcgc aactcggatg cctgccgtgc gaacaggatg
      421 ccacgatccc agtcgacgta cccggcgaac agtcccttcg agacgagatc gtacagatcg
      481 gaccggacgc tgtcgcgtgt tccctggatc tggagcgcca gatccgcgat gctgatctca
      541 ccggcgctct caacgatccc gagcatcttg cgctggcgct cggcgttggc gaactgcctg
      601 gcctcggcgc caccgcgaat gtacagaaag atacccccgg ccagcaacgg caggacgagg
      661 acgataaaga gcagcgcgcc gccaaagacc gccccggaga gccgcagacc tgactcgtcg
      721 tccgccaggc tggatgcaag ccaggcgacg gccacggcgc cgacgaggac gcccgcgacg
      781 accatgatga taccggcggt ccggccggtc aatgtcatcc gattcctcca agaacgtatt
      841 ccaccgcgct cgcgacgttg ttcgtcgtga cgagcttgag gcaggtgcga tcggggcagc
      901 ccgctggccg gccgagccga aatcctgtgt agatacatgg ctggcagggg agttcggcac
      961 agacgatctc caccgtaccg gatcttccga tcgggcgcca ggcgcgggcg ttcgacgggc
     1021 cgaagatcga gacaatcggc gttcccacgg cagcggcgag atggccgata aacgtgtcac
     1081 cgccgaccgc gacctgcgct ccggccagta cgacggccag ctcctccagt gaggttcttc
     1141 ccgttaggtc gatcgtcgac ggctcggtgg cccgaatcgt cgcggctgcc tcgctcgcgt
     1201 cctgagcgcc gaccacgagc gggataatgc cttgagccgc gagtctcgtc gcaaccgcac
     1261 cgaatcgctg gacgctccac tcacgcgccc gcgaatattg cccggtgacc ggaaagatcg
     1321 tggcgtacgg cgtcgacggt gtaccggctg gtggtcgagc ctgcggaacg gcgatgcacg
     1381 gcgttgcgtc aacgctgatg ccgccagcgg cgaccgcgac gtcgcgcatg taggtcgctt
     1441 cgtggcgggc gccaaagcct tcgtcgatga ctcgaacgct cagaaacgta ccccggccgt
     1501 tgtccaggcc gacgaccgat cgacagccgg ttgcctgggc gagggcggcg aactttcggg
     1561 caccgaacgc ggtcgtcagg tggtgaagga ggacgacgag atcatagcgc gctgatcgca
     1621 acgtcagcgc cagccggacc gcccgtactt gtgccgacgg gcgagtcaat ccggcgagcg
     1681 agtcgaacag ttgtttcgga aactcgattg gccgcaggcc atggccgatc agcgggagca
     1741 gtgcagctga tgatggcgtt gtcaggacgt cgatccgtgc ttcgggaaag gcggcccgta
     1801 gcgaccggaa ggccggctcg cacagcagga gatcaccgag atcggcgagc ttgatcgcga
     1861 gaatccgttc cggcttacga gcgcccatcg atcgccttcc ggatgacgtc caacgtggca
     1921 gtcgccgttt gctgccatgt gaaatttcga gcccgctcga ttccggccgc gactagttcg
     1981 gcacgccgtt gaggtttgat cgcagcaacg atcccgtcgc gaatcgaccg gatgtcgaat
     2041 ggatcgatca gaatggccgc accaccggcg acttccggca ggctcgatcg gctgctggta
     2101 acgaccggac agccacaggc catcgcttcc agcaccccca ttccgaaacc ttcgaatagc
     2161 gacggcagaa cgaacgcctc ggcgccatta tagagggcag gaacgtcagc gtcggcgacg
     2221 acgccgaggc gccggatgcg atcgctggcg ctgctgcctg cgatgcgggc ctctatcggc
     2281 tcgctgagcc agccgctcga accggcgatg accagtgtca gtccaggctc cagtgactcg
     2341 aatgcttcga cgagccgctc gacgttcttg cgcggctgga cggtgctgag gaagaacaga
     2401 tacggttgat tgatgcccag tcgggagatg accgcctgaa cggtgtcgac gtccagcggt
     2461 cgaaatcgtt ggtggtcaac gccggagtga atgacgctga tcttggccgg atcgacgcga
     2521 tagtgacgga ccagatcgtc gcgcgtctgg ccggagattg cgatgatctc ccgcgcggta
     2581 cgggcgttcc agcgagttgt caggtccagc attctgcgcg tgctggccgg gtgcgcgtcc
     2641 ggctcggcga gatagccgag gtcgtggacc gtaacgaccg agactgcggg atggacaagc
     2701 ggcagaacgt gcgacggcac gtagagcgcc gccgggcggt catgacgcat cgcacgggag
     2761 aggcccagat gggtccagag gcggggctgc tggatatgct ggtggctgac ggccggcccg
     2821 tcaagctcgg gcggtggcgc tccacgatgg tagagcctca gtggcggccg atcctcgact
     2881 gcgatcatgg cgcgaatgag ttcgaacgag taccactcgg ttcccgttcg cgccgcgact
     2941 cccgcgcggc tggcatcgac cgcgatctcg tcagcgtgca tggttggcca gcagcgctcg
     3001 ggcgacggca gcgacctcgt tcggctggat ggccagcatg cagccacagc cggaaccgcg
     3061 cccggccgag aggtcgccgc agcgcgggca gcgccaaccg gcgctgatga cgcggtggcg
     3121 gacggggtcg ccccacggac cgtaacgtac cggatcgctg gggccgaaga ggtggacgct
     3181 tggcgtgtcg gccgcgaccg cgagatggag cggaccacag tctggcccca gcacgagtga
     3241 cgctccgcgc aggatctcgg tcagaactgg cacggtcgtc tggccggcca gattgacggc
     3301 tcccgttccg tctcgggcga tcccatcagt gatgacacgt tccggcatcg aacctgtcaa
     3361 gacgattgaa tatccagcgt cggccagcgc gctcgcgacg tggcgccagc gttgagcggg
     3421 ccactccttg acggatgcgc ccgatccggg gtggatgacg acgtagccgc cgttgactcg
     3481 atgcgaccgc agcaatcggg tcgccagtgc cacagcgtcg ggggatggcg tgagatggag
     3541 cgggtcgctc agaaccgtcg ggacgcgcgc gtcatcgccg ttcgacgcga tccgaaatgc
     3601 gcggacgctg gcgtgatctc catcgtgcac gacggcagtt cgggtcgcga atgcgctggt
     3661 gcgcggatcg gctgccgtca ccacctctgg cgctatcagc gacgccagcc aggccgccca
     3721 ccagccatcg ggccgaagga cgtaggccga cctgggatgc agcgcacgca gtcgttgact
     3781 ctcggctgtc agctgccggt atggggccgt catcgacatc gatcgctcac gtgtgaagcc
     3841 agggaactcg accgtcagga tctcatcgac cggccatgac atttcggcga tctcgcgcga
     3901 ccacggtccc acgactgcga cgagacgact atctggaaag cgcagtcgga gatgtcgaac
     3961 cgccagctgg gacagaagga tatccccgag atggtcaggc tggatgatca ggaccgtgtc
     4021 ctcggtcggc tcccagcggc tcggacgcag gtgtgcagcg gccggcagca ggcgatcgcg
     4081 aaccgcagcg gtcagtcgtt gcatcgacgc cggtcctggt ggcgatcgat tgcttcacgt
     4141 gcagccctca gcactggatc gaacgtcacc aggttggttg agatcgggcc tggttcgtcg
     4201 gttctccggc gcaggtcgcg atcgacgatc gaccacgacg cagccggcgc gagtacgtgg
     4261 acgtccgggc cgagtggccg ataccggcgc gggttggttg gcccgaagat ggccactgtt
     4321 ggtgtgccga cggcggcggc gatgtgactg actccggtgt ccgggccgac gtagacggca
     4381 gcttgttcca gtatcgccgc gagcgtgccg atatcggttc tcccggcaag gatcgaccaa
     4441 tcctccagac cggcatcacg tgcgatttgg ctggctagcg caacatcgtc gggcccgccg
     4501 ctcaggagga ctgatatacc atcgcgcttc agttcgctgg cgagctgcgt gaacctatcg
     4561 gccggccagc gcttctcaag catgttcgct cccggattcc tcgcgccacc gggatgcagg
     4621 atggacagtg gggcagattg gtccggcaac aggcccttcg ctgcttgccg tgcaaccacg
     4681 gacggcgtga tgctgggagt tgttacgggt gttgaaatcc cgagttgacg catgacgtct
     4741 agatagacct ctgtctcgtg gcgtagttcc ggtgcccgcg tgtgaatcgc gaccgacttc
     4801 cgcgcgcgtg cgagacgcga ggcgccattt agcagccggg agcggtcgag gacgaccacg
     4861 aggtcgtatt gtccctgacg tagcacgcgc gatagctgta gcgcagaggg aacgtcgagc
     4921 gagtcgcgga cacgccggag atgcaatcgc gatcggaagg cgaacgcgct tcgggagtgt
     4981 gcgccgacga acacgtcgac ctcgcttgcc ggccaggccg ccagaaccgt gtcaatagct
     5041 ggcagcgcca tgacgcaatc gccgaggcag cacggcttga ggatggcgac gcgctctggt
     5101 gtcattcggc cgaagctcac gatggcgtgg agatgtctgc cacagcgaac atcaactcgc
     5161 ctcgaaccgc cagttcccca tcgacggtcg ccgtcgccgt acccatgccg acgccacgtc
     5221 gcatcttgcc gagcgtcact tcgagacgta acgtgtcgcc gggcttgacc tgacgcttga
     5281 agcgaacgcc gtcgatgccg gcgaaaaatg gcatctttcc cttgaactcc tccatcccga
     5341 ggatcgcgac ggcgccgacc tgagccaggg cttcgacgat gagaacgccg ggcatgaccg
     5401 ggtagtccgg gaagtgcccc tggaagaacg gttcgttggc cgtgacgttc ttgattccga
     5461 cggcggcaac gccgtactcg atctcgacga tccgatcgac gaggagaaac gggtaccgat
     5521 ggggaataat cgcctgaatc tgctgtgcat ccagcatggg atgtggatcc cttcacttgc
     5581 aacggtcaat gccgctctgg gagtctagga acgaggcgcc gaaattgtca atcaatgccg
     5641 ctcctgaatc agccggcggc gagcgaacgg tcgtcattgg ggctggtgcc tggggcacga
     5701 cgctggcgaa cctgatcgca tcgaccggcc gcgatgtgac actggtagca cactcccagg
     5761 cacatgccga acaggtccga ttggcacgcg agaacgagcg ctatctcccg gcgatccggc
     5821 tcagtgatcg tctgcttgtg actgatgatc ctacctcggt ggtttccggt gccagcatgg
     5881 ttgcgctggt cgtcccatcg cagcaaatgc gagcgagcgc ctcgaaggtc gcgccatttc
     5941 tcagcccgga agcagtttta gtgagctgtg ccaaggggct cgaactggga acgctactgc
     6001 ggatgactga ggtcgtcgcc gatgcaagtg gcgtcgcaac caaccgtatt tgcgcgctct
     6061 ctggtccgaa cctggcgcgc gagatcgcgc tcgggttgcc ggcatcggcg gtcgtggccg
     6121 gatctgatca gcgtgtttcg gagatggccc agctggcgtt gggggctccg tcgtttcggc
     6181 tttacaccag cgctgatgtc gtcggggtcg agctgggtgg agcgctgaag aacatcgtcg
     6241 cgcttggcgc gggggttgcc gacggactgc agctgggtca taacgccaag gccgccttca
     6301 tcacgcgtgg cctggcggag atgatccggc tcggcgtcgc tgccggagcg aatcccctaa
     6361 cctttggtgg actgagcggg ctcggtgacc tgatcgcgac ttgcgagagt cctctcagcc
     6421 gcaaccgcag ctttggcgag cggctgggtc gaggtatgtc gatcgaggaa gctgctgcgg
     6481 gaactccgca tgtcgttgaa ggcttgacga cggcacgcgc cgcgctgacg ctggcggaac
     6541 atcatcgagt cgaaatgccg attgcggcca ctgtcgtcag cgttcttgat ggacgaatgg
     6601 gggttcggga cgcgatcggc tcgttgatgt cgcgtggcgc ccgcccggaa ctggacgcgt
     6661 ttcgctagcc ggctgcgatc ggagaaacca acagttggcg acgatgtggc ccgacgccga
     6721 cgaagcggat cgggacgccg attagctcct cgatgcgctg gacgtacgct cgggcgttgg
     6781 cgggcaggtc gctgagtgcg gccgcgtcgg aggtgtcctc gtcccagccc ggtagttcct
     6841 cgtagaccgg tcgtacccgc tcgtagtcgc tgatacgggc cggtggcgcg gcgatcggtt
     6901 caccgccgag ctcgtaggac gtgcagatct tgaccgactc catcccgtcg agcacgtcga
     6961 actttgtcag tgcgatgtcg gagatgccgt tcagacgtgc ggtgaagcgc gcctgaacgg
     7021 cgtcgaacca gccggtccgg cgtggccggc cggtggtcgt gccgaactcg acgccccgct
     7081 cgcgcaggtg atcgccggtc gcgtcgagca gttctgtcgg gaagcctccg gcgccaacgc
     7141 gcgtcaggta cgccttgtag actccgatca cgcgtccgac ctgggttggc gcgatgccgg
     7201 ctccctggca gacgccagcc gcggttggtg atgaagacgt cacaaacggg taggtgccgt
     7261 agtcgatgtc gagcatcgcc gcttgcgcgc cttcgacgat gacatttgcg ccgttctcaa
     7321 tcgcgtcctg gacgtcagac tcgacttcgg cgaggtacgg cgcgagccgc tcgccgtagg
     7381 ccgcgtactc ttcggcgatc tggttgagat cgaacggctc gtgcttgtag accgcctgga
     7441 gcatggcgtt cttctccgcc agaaccggcg ccagcatccg gttgaggtgt tccggatcgg
     7501 tgatgtcggc cagccggatg ccgcgccggg cggccttgtc cgcatagcag ggaccattgc
     7561 ccgtctgggt cgtgccaatc atgtcgacac cccggctcga ttcttcaagc tgatcgatga
     7621 cggcgtggta cggcatgatc aggtggcaac ggctggagat acgcaggttg gacgagtcga
     7681 tgccgcgctc atccagacga tcgagctctt cgagcagcgt cttgggattc aaggcaacgc
     7741 ctgccccgat gatacagacg caatcagggt ggaggatgcc tgacgggatc aggtggaact
     7801 tgaagacgcc cttgtcagta acgacggtgt gcccagcgtt cgcgccgccg ttggcacgga
     7861 tgacgacatc ggcgctattg gcaagggcgt cgatgatctt gcccttgccc tcgtcgcccc
     7921 actggccgcc aagaacgatc gaaaccggca tcagaaccgt cccccggtga acacgaatcg
     7981 gtggcggggt ggtgcgagtc gtacccggct gaccgccaac caccgattct atcacgcgtt
     8041 catggtcact ccggctccct ggcgggcgtc ctggagctgt tcgaagagct cgcgttcgcg
     8101 ctccgagaga tcggtcggta gaacaacgtt gacacgcacg agcaggtccc cacgctcggt
     8161 cgagccgaca gcggctggcc agccgagctt gcgcaggcgg aagatgcgcc cgttctgcgt
     8221 ttcgggcggg atcgtcaacg ccacgcgccc tgtcatcgtc cgaaccagtg cttcgccgcc
     8281 gaggatggcc gtgtaaagcg gcaccgtcac ctcggtcttg agatcgctgc cttcgagcgt
     8341 gaacgctgga tccggtttga tgcgcacctt caggtagaca tcgccggccg gcccgccgtc
     8401 gcgcccgggc gagcccttgc cggccacgcg aacgcgctgg ccatcgcgga tccccgccgg
     8461 gatcgtaact tcaatccggc tacgcttcgt cgtgctgccc gacccctcac aggtgaaaca
     8521 gatcgaaccc tggatgatgc cggtcccgcc acagtccggg cagacttcag gcgtctgcat
     8581 ctcgaacgcg cgcgtcgtgc cggtgaacgc gtcgtgcagc gacacgtcga tcgggtactc
     8641 gtgatcgtcg ccccgacgag gagcgcggcg ctgcgtgcgc gacgaacggc cgcccgagcc
     8701 gaataacgtc tcgaagaagt ccgagaatcc ttcgccgccg gtcgtccggt agtcgaatcg
     8761 gacattcggg ttgccggccg cctgcccgct gaaccactgg gaaaagtcag atgctccacc
     8821 gctgtctgcc gcagactgat agcgctccca gtcggaaccg aagcggtcgt agcgagcacg
     8881 cttctcgggg tctgacagta cctgatgggc ctcgttgata gctttgaact gttcttcggc
     8941 atcggcattc ttgttcacgt ccgggtggag cttgcgagcc ttttcgcggt acgccttctt
     9001 gatgtcacct tcactcgcgg accgtgacac gccgagcact gagtaatagt cttgaaattg
     9061 catgatcggt tgctccagcg tcgtttacca tagtttcact tgtagtataa accatgagtc
     9121 atatcaacgc aaggtgacgc atgttacgcg ctgccagcct cgatgagttg gcgccgtcga
     9181 cggcggcgcc tgaagagcgt gaagatcagc gcgagcagca gaatgaggat gatcgcgatc
     9241 agaatcgggg cgatcagggc gatcaccgtc acgataatcg cggtgccgtc ctcgacggca
     9301 ctgacgatcg ggttcccgat tccgccggtc gtcgcggtga cgactggcct gaacgacgtc
     9361 ttggtcgtat ggatgccgcc ggccaccagc agaccaagga tcatcgcgat gatcggattg
     9421 atcgagtcga ctgtgtcggt gctggccatc agcaggattg cgccggccga cgggcggatg
     9481 gccgactgga tcagatcgtt gacatggtcg acgatcggga tcttgtcggc cagcagctca
     9541 atcccgagga agacagcgat gatggcgatc gcgatcggcg atgagagcat gtcgtacggt
     9601 tcggcgagct tgatcaaccc ggtgtaccga tcggcgagcg cgaccgccag caagggaatc
     9661 caggcattca ggccggccgg catcgccagc ccaagtccag tcagcaactc aaacactgcg
     9721 acggccgatc attggttgca tctgcgcgac agcaactaga acgccgtcgc ggtcgtcggc
     9781 ggaaggttcc tcacgccccg gtaagcctgc cctagcaggt gcgcctgcgc cagtgtcagc
     9841 cagggaccga cgatgaacag gccaacgaag caggtgacga caccggccac cattccggcg
     9901 actgacgccg ctgcatagat gagcgcacag agcaggagcg ggccggcgcc acgccgtgct
     9961 tcggcgaaga cagcaccgaa ctcaaacatc gacgagaact cgccgctcag cgcgtaacgc
    10021 gccgcgacga tctggcaagt aatggcggcg tagacgattc caagcgggat gaggcaggcg
    10081 aggccgagca ggaccgccac acccgagttg tcgatgatcg cgagagcgac gatcggacag
    10141 gcgagcagga agagcggcag ctcccagatg acgatgccga tgctcaccaa caggccgcgg
    10201 atgaagtaat cgccgaagtc gtcccactcg ggaagcggca gttcgttgcc ctggtagacg
    10261 cgcttgagga catcgagtgc gtatccatac agcgcgaggt tgagaagcgg caccagcgag
    10321 acggccgcgc cgatcagaat cttggcgatc cactttggat cggaggtaat gaatgtgtac
    10381 gcgcggccga tatccacgtt cttcctcacc ttgtgcgcgt tcggcaatca tggaagggat
    10441 gttcggttga atcccgacga tggcgcagcc tagcacccgt tggaaccgcg gtcaatcgcc
    10501 agcgctgatc tcttccgtcg gttgtgctgc gcgttgtctg tcaaccggcc acgcgacgac
    10561 gatcatcaac gcgatgatcg tgcagagcat caggctggca acgacccgct ggtcgagcgg
    10621 cgagatcgca tatggctgaa acgcgatcag gtgtccggcg atgtcggtgc cgaaccagcc
    10681 gtcgaggacc atatagagat tgttgatgcc gtcgggctgg tgatagaagc gctgtggatt
    10741 gatcgctccc gccgccacca ggaccatacc ggcacaccat agaccaacga tcgctgctcg
    10801 aacgggcgtg gagacgtgaa cgacgagggt tgccaggaca ccggccgcca gcggtgcaac
    10861 cggcaccagg tagcgcgccg gtggtcccca ttcgccccac cagacgcgat aggttgcgac
    10921 gacgacgaga taggggacga tcgccagagc tgccattcgc gcggttggcg atgacttgag
    10981 ccagaacgga atgcccgcca ggacaacggc gtagagcgga gctgcgatca ggagtcccca
    11041 ttgcgcgtcg agcagcaggc cgaatgtcgc gttgagcgtg ccttcaggtc cgttgaagcc
    11101 ggcgtgatcg gccgtactct gaaacgggct gccgtagagc cagaggttgt agccgaccag
    11161 gctcaaaccg ccgacggcta ctggagtcag cgccgcgatt gcgccggaat actcgcggct
    11221 cttccagaat ctgtagagca cgatgccggt caggacaacg gcagtcggag cgaatcgctg
    11281 gtgcagccat ggcaggaaac cgatggccat gccggtcagc acccagacgt agagcgcgtt
    11341 cgatggcgtc gccagccggc gtatcgcata gatcagcaac agtgccgccg gtatctccgg
    11401 gaagagtagc gacgagtacg gcgcgagcgg catcgagacc gccaacatga tcgcgatcgc
    11461 tgctgcccaa gcgggcgatg cattcacgtc atgcgccagc aggaacatgt tcgctgcgac
    11521 gaacaacgag cagatgagca cgacggcaac ggcgcccggt ctaccggcga tctcgtaggg
    11581 agtcgcgatc aggaatgaca ggccgagtcc atgtttagtg tgcaggccgt ttcgatcgga
    11641 cgttgcgggg tgcggcggta gcgttctcgg aaatgccggc cagccgtccc aaccagcagg
    11701 cagcggctca ggtgggtaga actcgtcata atcacgatcg gcgtagttgt tggattcatc
    11761 gagatctcga tcgttcatga tgctgagggc cgtcatcacg tagaacggct cgtcgcccgt
    11821 gagtggatcg agccgatcga gcactcggat ggcgagtgcg atctgggcga cagcaacgat
    11881 ggcgatgacc gcgaacagcc agcgccggtc ggatcgggtc accgggatga agccgaggtt
    11941 agcgttgaac gggcgaaggt actgctgatc accgcgagcg tcgccccaac cgtgttgacg
    12001 aacaggtcga acacgctggc gtcccgtccg ggcacgaatg actggtgaaa ctcgtcggtg
    12061 acgccgtaca gggcagcgcc aagaaccgct gccgccatcg tcgtgcggga tggccgagcc
    12121 gtcccggcaa acgcgaacag cagtagcagg cccaagacgc catagagcac catgtggccg
    12181 acaacggtca ggagcatgat cgagacgccg agggtacttg gaaactgttc ctgggacgac
    12241 atgaagaaga tgaacgccat ccaggcgact gccgggatga ttcgcacgat caattgtttc
    12301 aagatgccgt cactccagcc tgacgatacg cggtgacgag ccggtcggcg ctcgcctgaa
    12361 cgctgaactg ttgcaggaca cgcgcacggg ccgcttctga aagccgcgag atgcgatccg
    12421 ggtcacgcag gaggcgaacg gtcgactcgg cgaactcctc cgggtcagat cgatgcagca
    12481 atacagcaca gccgcctgca actgccggca cgtcgccaac ctccgaggcg acgaccgcac
    12541 agccagccgc cattagctcc aacagcttga ccgagttctt cgaccgtgcg atcagcgaat
    12601 cggcataggg atagatggcg atgtcggacg atccaagtag ttcaggaatg cggttccaag
    12661 cgacatatcc catttgatcc aaaactagat tcgcctttcc aatatcgcga gctggttcac
    12721 cgccgatgat tcgaacgacg accgacgtgt cggcggaaag gaaggcggag agcgatgcaa
    12781 gatatcgtgg caaccaatcg tgagcgaact ccgcaaaacg cgagtacaac acgatggttg
    12841 gcggtaggcc gactgccacg ggcatcggtt tccgagcctc aaataactgc ttcgcccacc
    12901 gatcggccaa accgtttggc acccacgtca cttgatgcgg cgtccgcgcc cgaagagcga
    12961 ttgcgcgaca cgcgagcaac tggcttgcag cagtgacggc cactgcgtcg cgcaacaggt
    13021 tgcgctcctg ccagtcgaac aagcgtcgct gaagcacgga gtagttgcct gcctggttcc
    13081 agccgccatc gccttcccag tcgtcgctgt caaccagcaa cggcgtcgat ccggagagta
    13141 acttcccggc gaggccgccg tagcccttcg gcttgaagac atgcactgct tgtggacgga
    13201 gtgccctgat tgcccgcgcc tgctgcagga ccgctgctgg aaatgaggtc acaccggtcg
    13261 cggaagtatt tgtcaacgaa actccgtcga tgacatcgac gacaccagct tcgccaggca
    13321 tatcccaggg gacggtcacg attgcgctgc gaatcccgcg tgacgtcagt tcgcgggcaa
    13381 gcggtagtgc acgcgcctgg agcgtcccga gccgccaggc cgcgaaggtt ccggccatta
    13441 cgatgtcaac ggattccgca cgccgctgca tggcaggcga tggtaccgga cgagcgcggg
    13501 ccgttgccac gtcagacgcg aagcgggcgg gacacatacg catcccaccc gctcaatcgc
    13561 tcctgctatg tgagccgctg ttactgaatg accggccgga tgacggtcac gagcttgccc
    13621 tgaatctcga cgttgtcggc cgtcgtgtag atcgggtcca tcgtgacgtt ggccggctgg
    13681 aggcggacct ggccgttgtc gtgatagaag cgcttgaggg tcgtctcctt ctcgcccttc
    13741 agccagacag cgacggtgtc accgttctca cagaccacct gatgacgtaa gacaacaacg
    13801 tcgccatcgt tgatgagcgc atcgaccatc gagtgcccct tgacgcgcag ggcgaagagc
    13861 ccctcggtgc gcgggccgag cacatcggaa ctgatctcga tcgtgtcgag atactcctgg
    13921 gtggcgagat cgtctggaac aggaatcggc tcaccggcgg cgattcggcc aacgactggg
    13981 atcgagataa tgccacgccg ggccagcggg cgaccgacga gttcaatgcc gcgcgagatg
    14041 ttgcggttac gccggatcag gttgcgctgc tcgaggacgt tgaggttgta atcaacgacg
    14101 ctcgtcgagg agatggcaag cccctcctga atctcgcgaa tcgtcggcgg gtaggagtgt
    14161 tcatcgctga aactctcgat gtacccaaga atatcctgct gccgtcgtga gagctgcttc
    14221 ataacctcgt tacccccggt tggacgatcc gcgtggaccc ggaccactcc cgggtcgtcc
    14281 cgtttccgcc agtataatct cgaacatctg ttcgtgtcaa tcatgtatcc cgatcaagcg
    14341 atggtgcttt ttggctattc gctcgggacg ttggattctc tacactggaa atcgatccgc
    14401 aagtgacgtt gtcaccagcg tgcgtcatgc acgacatcat cggcaggtag gacatgaacg
    14461 ttctggactt tgggatcgtc ctcgttttcg caatactgat catggtcggc tttttcggcg
    14521 gcgtcggtag ggtgactgcc gggttctttg ccgtctattt cgcttcgatc gtttctgcgg
    14581 ccttctatga ggggctgggc gacatcttca tcgacagggt cagtgacatg cgcgaagcga
    14641 ccgcctacct gctgtcgttc atgctgctgt tcgtctcctt atcgggcgcg ttcttctggg
    14701 ccatcggcgc aaccatcaaa gctgtcgaac tacggcgtgg caggttcgcg atcatggata
    14761 atgttggtgg cgcgacgatg gcactagtcg tcggcgcggt ggccgtcgcg atgacactct
    14821 ccgtgtcggt cattatcctt ggcgcattca accagtccgc tgtggtgaac ggtgatgaga
    14881 atcttggcac gctgggcaag cagatacgcg gctccgaact ggttccgatg ttcctgaagc
    14941 ttcaggaacc catcctcgtc gtctaccgcc catggtttcc cgagggcctt ccgccgattc
    15001 tcaaaacccc tccccagtct gtgacctaga cgaccggaag ggtagtttgg caaccgacat
    15061 cctgacgacg ttggagttcg accgtgttcg ggagaacctc cagcgtcatt gtcagttttc
    15121 gctcagtgtc gagcgtgcgg gcgaacttgg tccgagttcg gacccgaccc gcgtccgcta
    15181 cctgctggat gtcaccggcg aggcgtacat gctgatcgaa gcctatccgg cattcggtgt
    15241 gcgcggcgct cgcgatatcc gccgccacct gcaacgcgcc gagcttggcg gcatgctaca
    15301 accgactgat ctactggaag cgctcgacat gatcacggca tcgcggacga cacggcggac
    15361 cttcctgcgt atggaagacg cggtcgaacg ctttccatcg atcgacgagt tcgtgagctt
    15421 catcgtcgag cttcctgacc tcgaagcgga gctgcaacgc tccatcagcg aacgcggcga
    15481 ggtgcttgac actgccagtg aagcgttgag gaagatccgg caggagctcc gctcggccca
    15541 cgcacgactg atcgaacgcg tcacccgcta catccaggga tcgcaatact cggc
//
LOCUS       DASUGJ010000314         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_186016, whole genome shotgun sequence.
ACCESSION   DASUGJ010000314 DASUGJ010000000
VERSION     DASUGJ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186016"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..>2044
                     /locus_tag="VFV93_07025"
     CDS             54..>2044
                     /locus_tag="VFV93_07025"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX5165130.1"
                     /translation="MAKNPLQRLADYGQSVWYDNLSRDLIESGDLQKLIDEAGVVGVT
                     SNPTIFDKAISGSSDYDEQIREFAEAGRTTDEVFEELVIRDIQEAADVLAPVWERRSK
                     TLADGFVSLEVSPTVAHDTEKTMADARRLFARVDRPNVMIKIPGTVEGVPAIEQMLYE
                     GVNINITLLFALDAYRQVMEAYLRALERRVEEGKAIDNIHSVASFFVSRVDTEADKRL
                     EAIIKDEAGSERAEKAKALRGKLAIANAKLAYLAFSEIFESERFQRLADMGARLQRPL
                     WASTSTKNPEYSDTLYVDELIGPDTVQTLAPASIAAFRDHGTLGNTLQSNLDDARKVF
                     AEFEELGVSVDDITDTLVREGVASFAKSFEGLLAGLDEKRERFKAEAREARMAALDGF
                     APKVQQALDELASQDVAARLQDQDPSLWSDDSKQQNSIAQRLGWLPVVHAMVSEARSG
                     FFEEFAAEVKQRGYEHAVLLGMGGSSLAPEVFARVYGRQPGFPELTVVDTTNPDTIKR
                     VADRVRGRKTLFIVSTKSGTTVETMSLYHYFLKEQGGDGSNFVAVTDKGTPLEAEAKK
                     AGFWRVFCNPSDIGGRYSALSYFGLVAAAVAGVNIDKLLHRASIMLPIHDNDHPGIWL
                     GAVMGAAQREGRDKLTLIASPGWEPFGDWLEQLIAESTG"
BASE COUNT          394 a          632 c          694 g          324 t
ORIGIN      
        1 ctggcacgcc agacgcggga atagcgatag cagcatgctg aaaggaaccc cagatggcga
       61 agaacccact gcaacgacta gcggactacg gccagagcgt ctggtacgac aatctcagcc
      121 gcgatctgat tgaatccggc gacctccaga agctgatcga cgaggccggc gtcgtcggcg
      181 tgacctcgaa cccgacgatc ttcgacaagg ccatcagcgg ctcgtccgac tacgacgagc
      241 agatccgtga gttcgccgag gccggccgga caactgacga agtcttcgag gagttggtca
      301 tccgcgacat tcaggaggcg gcggacgtgc tggctccggt ctgggaacgc cggtcgaaga
      361 cgctggcgga cggcttcgtc tcgctggaag tctcgccgac cgtcgcgcac gacaccgaga
      421 agacgatggc cgacgcgcgg cggctgttcg cgcgggttga ccggcccaac gtgatgatca
      481 agatccccgg cacggtcgag ggcgtcccgg cgatcgagca gatgctctac gagggcgtca
      541 acatcaacat cacgctgctc ttcgcgctgg acgcctaccg gcaggtgatg gaagcctacc
      601 tgcgagcgct ggagcggcgc gtcgaggagg gcaaggcgat cgacaacatc cactcggtcg
      661 cgtcgttttt cgtcagccgg gtcgacaccg aggccgacaa gcggctggaa gcgatcatca
      721 aggacgaggc cggtagcgaa cgggccgaga aggcaaaggc gctgcgcgga aagctggcga
      781 tcgcaaacgc gaagctcgcc tacctcgcgt tcagcgagat cttcgagtca gagcgcttcc
      841 agaggctggc cgacatgggc gcgcggctgc aacgcccact ctgggccagc accagcacca
      901 agaaccctga atattccgac acgctctacg tcgatgagtt gatcggcccg gacacggtcc
      961 agacgctggc gccggcgtcg atcgcggcct tcagagacca cggcacactc ggcaacacgc
     1021 tccagtcgaa tctggatgac gcgcggaagg tgttcgccga gttcgaggag cttggtgtct
     1081 cggttgacga catcaccgac acgctcgttc gcgagggcgt tgcttcgttc gccaagtcgt
     1141 tcgaggggct gctggccggg ctggacgaga agcgcgagcg gttcaaggcc gaggcccgcg
     1201 aggcgcggat ggctgcgctg gacgggttcg cgccgaaggt ccagcaggcg ctcgacgagc
     1261 tcgcatcaca ggatgtcgct gcgcgattgc aggatcagga cccgtcactc tggtccgacg
     1321 acagcaagca acagaactcg attgcccagc gcctcggctg gctgccggtc gtccacgcga
     1381 tggtgtcgga ggcgcgcagc gggttcttcg aggagttcgc cgctgaagtg aagcaacgcg
     1441 gctacgagca cgccgtcctg ctcgggatgg gcggctcgtc gctggcgccg gaagtattcg
     1501 ccagggtcta cggccggcag ccgggcttcc ccgagttgac cgtcgttgac acgaccaacc
     1561 cggacacgat caagcgggtt gcggaccgcg tgcgaggaag gaagacgctc ttcatcgttt
     1621 caacgaagtc cggcacgacg gttgagacga tgtcgctgta ccactacttc ctgaaggagc
     1681 agggcggcga cggctccaac ttcgtcgccg tcaccgacaa gggcacgccg ctggaggccg
     1741 aagcgaagaa ggctggcttc tggcgcgtct tctgcaatcc gtccgacatc ggcggtcgct
     1801 actcggcgct gtcgtacttc ggactggtcg cggcggccgt tgccggcgtc aacatcgaca
     1861 agttgctgca ccgggcgtcg atcatgttgc cgattcatga caacgaccac cccggcatct
     1921 ggctgggggc ggtgatgggt gccgctcagc gagaaggccg cgacaagctg acgctgatcg
     1981 ccagtcccgg ctgggaaccg ttcggtgact ggctggagca gttgatcgct gagagcaccg
     2041 gcaa
//
LOCUS       DASUGJ010000315         7458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1853189, whole genome shotgun sequence.
ACCESSION   DASUGJ010000315 DASUGJ010000000
VERSION     DASUGJ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853189"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFV93_07030"
     CDS             complement(<1..719)
                     /locus_tag="VFV93_07030"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5165131.1"
                     /translation="MIPIGDDNRGSRRPPIVVIILIAINVLVFVYQLTLDSSGSEGPA
                     CEVMVAEGRFGQGTQLECFVYRYGVRAYELSHVEDVAPTIALPIFATVLTSMFMHGGW
                     LHIGGNMLYLWIFGDNVEDAMGHLRFLIFYLLTGIGAVGLQVAIDRNALVPMVGASGA
                     IAGVLAAYLALFPSGRVRVLVFLGFFVTVLALPALIVIGFWIVLQFFSGFASLGARTQ
                     DSGGGVAYFAHIGGFLAGLALV"
     gene            complement(831..956)
                     /locus_tag="VFV93_07035"
     CDS             complement(831..956)
                     /locus_tag="VFV93_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX5165132.1"
                     /translation="MLTRARTATKFFTYGLLIGVLFAPGSGEETRQRLKSWITRR"
     gene            complement(1069..1686)
                     /locus_tag="VFV93_07040"
     CDS             complement(1069..1686)
                     /locus_tag="VFV93_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX5165133.1"
                     /translation="MTAHERFQFPGTFEIWEGHMALSRSDESRHDFAFIAGAVIGAIA
                     GALVTLALTPMSGADTREKLRSRAGELGPIRERAMEAAAPVREKAGSIASTAAEKGKT
                     LAATGKERAVDLAAKAPIGRTHDGVELNDMSAEEAGSMLFGSQPGAQPGGTVTPGALA
                     DAGADAIDASMPETSGDEPTAHSQDPAEGARTAAGETGNGRISSS"
     gene            1956..3101
                     /locus_tag="VFV93_07045"
     CDS             1956..3101
                     /locus_tag="VFV93_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5165134.1"
                     /translation="MAQAMDVQTFREEFPVTQDWSYLNHAAYGPFPQRTVRAVTGWAE
                     GLAQAPTFFGPEREKIPGEAAEYVAGLAGATGDMVAWVPSLADGMNLLAHGVDWQDGD
                     NVLIPVDEFPSVVYPFLNLQSKGVELRYVAKNTEGRTDPNLIEAAIDDRTRAVALSYV
                     EFMDGYRNDLKALGSLCKDRGVELFVDVTQALGAQPIDLPQTGVSAVAAHGYKWLMAG
                     FGVGCVIFNPDAIERIKVTYAGRLSVKGGFEDSEYRLDWRDGAARYQTGGLNVLGLTA
                     LHASMTLVTQAGPEWTREHTLGLLDKLVEGVQGMGYEVASDLTRERRSQIMAFTSGDH
                     ARDNELVEQLTRSNVAVTLRGRGVRVSPYFYNTDDDIERLLEAVPPR"
     gene            3509..4705
                     /locus_tag="VFV93_07050"
     CDS             3509..4705
                     /locus_tag="VFV93_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5165135.1"
                     /translation="MQTDAPSIVIIPTYNEHETLVPLVQQVLDAAPGTDVLIVDDASP
                     DGTGALADSLAAVEERVDVIHREGKQGLASAYREGFQHALDRGYELITQMDADFSHRP
                     EDLPRLLEGATDADVVVGSRNVAGGRTEGWPWLRKLVSRGGSVYTRTLLRLPIHDCTS
                     GFKCIRRRALASIDINDVSSAGFGFQVEMNFLFHRAGMQLAEVPIVFPNRTAGESKMS
                     RDIFMEAMTLVWRLRWANRPRAGQAAQRVSNRSRRLLARHGSRFLRFAAVGGAGVAVN
                     SGLLYIFVSQAGLAPLIAAGVATEVSILSNFALNDAWTFRGVRGNVSRIASAARYNLN
                     ALGGFGLSLAVLAFLTQTTGIHYMLANLFAIGSATLWNYVANVRWTWPTLQTTNQSAR
                     RGAGRG"
     gene            4698..6011
                     /locus_tag="VFV93_07055"
     CDS             4698..6011
                     /locus_tag="VFV93_07055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5165136.1"
                     /translation="MAENLLVLAILLFSLALTAQAVFTLMLMLYTWALPDRLRQASSP
                     EAYAPPQLSFTAILPARHEQDVIAQTIQRVWETRYPHELIEVVVVCEAGDRATIAEAQ
                     RAIDAIGHPNLRVVTFDGQPINKPRGLNVAFHETRNDVVTIFDAEDDVHPEMFWIVNT
                     IMSNEDAGIVQAGVQLMDFRSRWFSVHNVLEYFFWFKSRLHFHARIGMVPLGGNTVFV
                     RRPLLQAVGGWDEHCLTEDADIGLRLSALNNRIAVTYDARHTTREETPPTLGSFVRQR
                     TRWNQGFLQILRKGTWHDLPTPGQRLLAIYTLAFPFIQAVVGVLWLPALLAMFFLEMP
                     VVVAMLSLLPLYALGFQYITNVVGLFEFASTYGMRVTLRDLAILTAGFLPYQVLLSAG
                     AVRAVYRELRGLKNWEKTAHTGAHRGARDPLPARTNRTRPALRGD"
     gene            6014..>7458
                     /locus_tag="VFV93_07060"
     CDS             6014..>7458
                     /locus_tag="VFV93_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165137.1"
                     /translation="MLRQPTRYERAQPRPVVRFGPTATAAGDAIRNWLRHDSIAKLAL
                     AFVLLTAGLFTGINIFNYPQFESDEGTYIGSAWAMMVKDQLSYYTYTYDHPPLGWLQL
                     GVWARLTGGFDTFGMSVNSGRVLMLGVAILSTLLIFQIVRNATERVSAALFGSILFAV
                     SPLAVSLHRQVWLDNLATLWLLVSLYLLVTARERMGRIVLSSLTFGVMFWTKEVFIAF
                     LPGMLLLAWAAAHPVQRRFVTVLWGSLAAASVSLFVVLAMLKNELLPPGVLGSNSTPH
                     VSLVDTYRYQASRGGGGSLLSADADFWTFMGQWRDAAPFLIVGGLLVTLLGLLLWRRQ
                     PLSFSLSVLVLTYVLFLGRGGVVLYYYIIPLIALLSLAIGVATGMLLNQIPRHSAPQR
                     VMATLVVAIALLTAQQSVGANHNNFHVDSTSSQRVAASWIADNLPRDNTIIMDSYLWT
                     DLRLPSFTDGRPFPNAHYYWPALQDPAIRDAV"
BASE COUNT         1243 a         2555 c         2361 g         1299 t
ORIGIN      
        1 acgagggcga ggccggccag gaagccgccg atgtgggcga agtaggcgac gccgcctccc
       61 gaatcctgcg tgcgggcgcc gagcgaggcg aagccgctga agaattgcag gacgatccag
      121 aacccgatca cgatcagggc cggtaacgcc agcaccgtca cgaagaagcc caggaagacg
      181 agcacccgga cccgcccgct ggggaagagc gccagatagg ccgccagcac gccggcaatc
      241 gccccgctgg cgccaaccat cggcacgagc gcattccggt cgatcgccac ctggaggccg
      301 acggcgccga tccccgtcag caggtagaag atcagaaagc gcagatgccc catcgcgtcc
      361 tcgacgttgt cgccgaagat ccataaatag agcatgttgc cgccgatgtg cagccagccg
      421 ccgtgcatga acatcgatgt caggaccgtc gcgaagatcg gcagtgcgat cgtcggcgcg
      481 acatcctcga cgtgcgacag ctcgtaggcg cggacgccgt agcggtagac gaagcactcc
      541 agctgggtgc cctgcccgaa gcgcccctcg gcgaccatca cctcgcaggc cggcccctcg
      601 gagccgctgc tgtccaacgt caactggtag acgaaaacga ggacgttgat cgcgatcagg
      661 atgatcacga caatcggcgg ccggcgcgaa ccccgattgt cgtcaccgat cggaatcacg
      721 cgtgaagcct gtggtaatag gtatcaggtg ataggtgata ggagctttcg ctcccatacc
      781 ccaccagcat cactcgactc caactcctat tacctatcac ctgatacctg ttacctccgt
      841 gtgatccacg acttcagccg ctggcgcgtc tcttcgccgc tgccgggggc aaagaggacg
      901 ccgatcagca ggccgtaggt gaagaacttg gttgccgtgc gcgcgcgggt cagcacgtgc
      961 gccttccttc ctggtcgtgt ctggatatca gccggcagcg atcagcaggc cggagcggat
     1021 acattcgatc cgctccaacc ccaactgaac gtcgctaccg tccggttgct agctgctgct
     1081 gatgcgtccg ttaccggtct cgccggcggc agtgcgtgcg ccctcggccg gatcctggga
     1141 atgggccgtt ggctcgtcgc cactcgtttc cggcatgctg gcgtcgatgg catccgcgcc
     1201 ggcatcggcc agcgccccgg gcgtgaccgt cccacctggc tgcgcgcccg gctggctgcc
     1261 gaacagcatc gatcctgcct cctcggcgga catgtcgttc aactcaacac cgtcgtgtgt
     1321 tcgcccgatc ggcgccttgg cggccaggtc aaccgcgcgc tccttgccgg tcgccgccag
     1381 cgtcttgccc ttctcagccg ccgtcgatgc gatcgagccg gccttctccc gaaccggtgc
     1441 ggcagcctcc atggcccgct cgcgaatcgg gccaagctcg cccgcccggc tgcgcagctt
     1501 ctcgcgcgtg tctgcgccgg acatcggcgt cagcgccaat gtcaccaacg cccctgcgat
     1561 tgcaccaatc accgcaccgg cgatgaaggc gaaatcgtgc cggctctcat cggaccgtga
     1621 tagtgccata tggccctccc agatctcgaa cgtgccgggg aactgaaaac gctcgtgcgc
     1681 cgtcacggat atcgtgacat ccccgacacc cccagtatcg catgcgtctg gttcactggg
     1741 acgtctgtat caagattgat gccgccgcgc gggattcggg attggggatt cgggactggg
     1801 aggcggcggc atgccttccc ggatatgaca cgatcgtgca gcaaagagac gaggccgtct
     1861 cccagccccg aatccccaat cccgaatcac gcaacttcgt cgtatcatcc ccttcatcga
     1921 ctcgaagact cgcacgacag cggaggagtg ggagagtggc gcaggcgatg gatgtgcaga
     1981 cgtttcgcga ggagttcccg gtcacgcagg actggtcgta tctgaaccac gccgcctacg
     2041 ggccgttccc gcagcggacg gttcgggcgg tgacgggctg ggccgagggg ctggcccagg
     2101 cgccgacctt cttcggaccg gagcgggaga agattccggg cgaagcggct gagtacgtgg
     2161 ccgggctggc tggggcgacc ggcgacatgg tcgcctgggt gccatcgctg gcggacggca
     2221 tgaacctgct ggctcacggc gtcgactggc aagacggcga caacgtcctg atcccggtcg
     2281 atgagttccc ctcggtcgtc tacccattcc tcaacctgca atcgaagggc gtcgagctac
     2341 gctacgtcgc gaagaacact gaggggcgga ccgaccccaa cctgattgag gcggccatcg
     2401 atgaccgaac ccgggccgtc gccctctcgt atgtcgagtt catggacggc taccgcaacg
     2461 acctgaaggc gctcggctcg ctctgcaagg atcgcggcgt cgaactgttc gtcgacgtca
     2521 cccaggcact cggcgcccag ccgatcgacc tgccacagac cggcgtcagc gccgtcgctg
     2581 cccacggtta caaatggctg atggccggct tcggcgtcgg ctgcgtcatc ttcaaccccg
     2641 acgcgatcga gcggatcaag gtgacgtacg ccggccggct ctcggtcaag ggcggcttcg
     2701 aggattccga ataccggctc gactggcgcg acggcgccgc ccgctaccag accggcgggc
     2761 tgaacgtgct cgggctgacg gcgctgcacg cctcgatgac gctcgtcacc caggccgggc
     2821 cggagtggac ccgcgagcac acgctcggcc tgctcgacaa gctggtcgaa ggtgtccagg
     2881 gcatgggcta cgaggtagcc tccgacctga cccgcgagcg ccgctcgcag atcatggcct
     2941 tcaccagcgg cgaccacgcc cgcgacaacg agctggtcga gcagctgaca cgctccaacg
     3001 tcgccgtcac cttgcgtggc cggggcgttc gcgtctcgcc ctacttctac aacaccgacg
     3061 acgacatcga acggctgctg gaggctgtgc caccgcgcta aacgccccct gaccgaccct
     3121 gacagcgacc caatccgacc cggctgagcg taaccgtcag ccgggtcgat cgttacttgt
     3181 cacatgtgcc ggtggatggg ttgccacctg ccgaatgtta tgtctttacg acatcgggat
     3241 catgattcgc aacttttacg aggcatgtgc attgcgacct gcgaacactt tggtcctggc
     3301 cttggggagc gctgccggaa tcagacatcg gcggcggtga ggccgggacg cagcaagggg
     3361 ccacggggag gaacctggca agggaaggta tccgggcacg caaccacgcg ctgcccgatc
     3421 acatcacgac accacgcgaa gcacgaggag cgacgacgga tgctgagggt cagcatccgg
     3481 atctgatctg acctggagca atgtgtcaat gcagacagac gcaccatcca tcgtgatcat
     3541 tccaacctac aacgaacacg aaacactcgt accgctcgtc cagcaggttc tcgacgccgc
     3601 gcccggcacg gatgtcctga tcgtcgacga cgcatcgccc gacggcaccg gcgccctcgc
     3661 cgacagcctc gccgccgtcg aagaacgggt cgatgtgatc caccgcgagg gcaagcaggg
     3721 gctggccagc gcctaccggg aagggtttca gcacgcgctc gatcgcggct acgagctgat
     3781 cacacagatg gacgccgatt tctcgcatcg cccggaggat cttccacgcc tgctcgaagg
     3841 cgcgacggac gccgatgtcg tcgtcggttc gcgcaacgtg gctggtggcc gtaccgaggg
     3901 ctggccctgg ctgcgcaagc tggtcagtcg tggcggcagc gtctacacgc ggacgctgct
     3961 gcggctgccg atccacgact gcacgagcgg attcaagtgc atccgccgtc gggcgctggc
     4021 gagcatcgac atcaacgatg tctcgtcggc cgggttcggc tttcaggtcg agatgaactt
     4081 cctctttcac cgcgccggga tgcagcttgc ggaggtgccg atcgtcttcc ccaaccgcac
     4141 agcgggagag tcaaagatgt cccgcgacat cttcatggag gcgatgacgc tcgtctggcg
     4201 gctgcgctgg gccaaccgcc cgcgcgccgg ccaggcagca caacgcgtca gcaacaggag
     4261 ccgccggctg ctggcgcggc acggtagccg attcctgcgc ttcgcggctg tcggcggcgc
     4321 cggtgtcgcc gtcaactcgg gcttgctgta catcttcgtc agccaggccg ggctcgcacc
     4381 gctgatcgcc gccggagttg cgaccgaggt ctcgatcctg agcaacttcg cgctgaacga
     4441 tgcctggacg tttcggggcg ttcggggcaa cgtgtcgcgc atcgccagcg cggcccgcta
     4501 caacctgaac gcgctcggcg ggttcggtct gtcgctggcc gtgctcgcct tcctgacgca
     4561 gacgacgggc atccactaca tgctcgccaa tctgttcgcg atcggctccg cgacgctctg
     4621 gaactacgtc gccaacgtgc gctggacctg gccaaccctc cagacgacga accagagcgc
     4681 gcggagggga gccgggcgtg gctgagaacc tgctcgtcct ggcgatcctg ctcttctcgc
     4741 tggcgctgac ggcacaggcc gtcttcacgc tgatgctgat gctctacacc tgggcgctgc
     4801 ccgaccggct gcgccaggca tccagccccg aggcgtacgc cccgccgcaa ctgagcttca
     4861 cggcgattct gccggcccgc cacgagcagg atgtcatcgc tcagacgatc cagcgcgtct
     4921 gggagacacg ctacccacac gagctgatcg aggtcgtcgt cgtctgcgag gccggcgacc
     4981 gcgcgacgat cgccgaggcg cagcgggcga tcgacgcgat cggccatccg aacctgcggg
     5041 tggtgacctt cgacggccag ccgatcaaca agccgcgcgg gctgaatgtc gccttccacg
     5101 agacgcgcaa cgatgtcgtc acgatcttcg acgccgagga cgacgttcac ccggagatgt
     5161 tctggatcgt gaacacgatc atgtcgaacg aggacgccgg catcgtccag gccggtgtgc
     5221 agttgatgga cttccgctcg cgctggttct cggttcacaa cgtgctggag tacttcttct
     5281 ggttcaagtc gcgcctccac ttccatgcgc ggatcgggat ggtcccgctt ggcggcaaca
     5341 ccgtcttcgt ccgccggccg ctcctccagg cggttggtgg ctgggacgag cattgcctga
     5401 ccgaggacgc cgacatcggc ctgcgcctct cggcgttgaa caaccggatc gccgtcacct
     5461 acgacgcgcg ccacacgacc cgtgaggaga cgccaccgac gctgggcagc ttcgtgcgcc
     5521 agcgaacgcg ctggaaccag ggattcctgc agatcctgcg caagggcacc tggcacgatc
     5581 tgccgacgcc gggccagcgc ctgctggcca tctacacgct ggcattcccg ttcatccagg
     5641 cggtcgtcgg cgtcctctgg ctgccggccc tgctggcgat gttctttctt gagatgccgg
     5701 tcgtcgtcgc gatgctgtcg ctgctgccgc tctacgcgct cggcttccag tacatcacca
     5761 acgtcgtcgg gctgttcgag ttcgccagca cctacggcat gcgcgtcacg ctgcgcgatc
     5821 tggcgatcct gactgccgga ttcctgccct atcaggtgct gttgagcgcc ggtgccgtcc
     5881 gcgccgtcta ccgggagcta cgcgggctga agaattggga gaagacggcc cataccggcg
     5941 cccatcgcgg tgcccgcgac ccgcttccgg ccagaacgaa ccgaacccga ccggcactac
     6001 gaggggacta atcatgttgc gtcaaccgac ccgctacgaa cgggcgcagc cgcgccctgt
     6061 cgttcgcttc ggcccgaccg cgacggccgc cggtgatgcg atccgcaact ggcttcgtca
     6121 cgacagcatc gcaaagctcg cgctggcgtt cgtgctgctg acggccgggc tgttcaccgg
     6181 catcaacatc ttcaactacc cgcagttcga gtccgacgag ggcacctaca tcggctcggc
     6241 ctgggcgatg atggtcaagg atcagctctc gtactacacc tacacctacg accacccgcc
     6301 gctcggctgg ctccagctcg gtgtctgggc gcggctgacc ggcggcttcg acacgttcgg
     6361 gatgtcggtc aactccgggc gggtgctgat gctgggcgtc gcgatcctct cgacgctgct
     6421 gatcttccag atcgtgcgca acgcgaccga gcgggtatcg gccgcgctgt tcggttcgat
     6481 cctcttcgca gtctcgccgc tggccgtcag cctccatcgt caggtctggc tcgacaacct
     6541 ggcgacgctc tggctgctgg tgtcgctcta cctgctggtg acggctcgcg agcgaatggg
     6601 ccgcatcgtc ctcagtagcc tgacgttcgg cgtgatgttc tggacgaagg aggtcttcat
     6661 cgcgttcctg ccggggatgc tgctgctggc ctgggcagcc gcccatcctg tccagcgccg
     6721 cttcgtgacg gtgctgtggg gatcgctggc ggccgcgtct gtctcgctct tcgtcgtcct
     6781 cgcgatgctc aagaacgagc tgctaccgcc gggcgtgctc ggcagcaact cgacgccgca
     6841 cgtcagcctc gtcgatacct accgctacca ggcgtcgcgt ggcggtggcg gctcgttgct
     6901 ctccgccgac gccgacttct ggacgttcat ggggcagtgg cgggacgcgg cgccgttcct
     6961 gatcgttggc gggctgctgg tgacgctgct ggggctgctg ctctggcggc ggcagccgtt
     7021 gtcgttcagc ctgtcagtcc ttgtcctgac ctacgtgctc ttcctgggac gtggcggcgt
     7081 tgtcctctac tactacatca tcccgttgat cgcgctgctg tcgctcgcga tcggcgtcgc
     7141 tactggcatg ctgctgaacc agatcccgcg tcactccgcg ccacagcgtg tcatggcgac
     7201 gctcgttgtc gcgatcgcgc tgctgacggc ccagcaatcg gtcggagcga accacaacaa
     7261 cttccatgtc gactcaacca gctcccagcg ggtggccgcg agctggatcg cggacaacct
     7321 gccgcgcgac aacacgatca tcatggactc gtatctgtgg accgacctgc ggctgccgtc
     7381 cttcaccgac ggccggccat tcccgaacgc ccactactac tggccggcgc tccaggaccc
     7441 cgccatccgt gacgcggt
//
LOCUS       DASUGJ010000316        11390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1993170, whole genome shotgun sequence.
ACCESSION   DASUGJ010000316 DASUGJ010000000
VERSION     DASUGJ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993170"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /gene="tuf"
                     /locus_tag="VFV93_07065"
     CDS             complement(<1..1175)
                     /gene="tuf"
                     /locus_tag="VFV93_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX5165138.1"
                     /translation="MAKQRFERTKPHINVGTIGHVDHGKTTLTAAITKTLALRGEASF
                     RSFDSIDNAPEERERGITIAISHVEYETDNRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAPDGPMPQTREHILLARQVEVPAMVVFLNKVDMMDDEELLELVELEVRELLSN
                     YNFPGDEIPIIRGSALAALESTSTDPSAPEYAPIWELMQAVDDYIPTPERAVDQPFLM
                     PVEDVFGIKGRGTVVTGRVERGRVKTGETIEIIGIKDTRSVVVTGVEMFQKTLDEGVA
                     GDNVGCLLRGIDREEVERGQVLAKPGSIKPHTTFAAEVYVLGKDEGGRHTPFFPGYRP
                     QFYIRTTDVTGAIQLPEGVEMVMPGDNIQMQVELIQPVAIEEGLRFAIREGGRTVGAG
                     "
     gene            complement(1309..1390)
                     /locus_tag="VFV93_07070"
     tRNA            complement(1309..1390)
                     /locus_tag="VFV93_07070"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1354..1356),aa:Tyr,seq:gta)
     gene            complement(1392..1467)
                     /locus_tag="VFV93_07075"
     tRNA            complement(1392..1467)
                     /locus_tag="VFV93_07075"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1432..1434),aa:Thr,seq:tgt)
     gene            1688..1957
                     /gene="rpsO"
                     /locus_tag="VFV93_07080"
     CDS             1688..1957
                     /gene="rpsO"
                     /locus_tag="VFV93_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX5165139.1"
                     /translation="MVLQKSQKDDIVESYRTHDTDTGSPEVQIALLTERINGLIEHLR
                     EHAHDHHSRRGLLKLVGRRRRLLAYLSEKDTDRYRALIARLGLRR"
     gene            2308..4656
                     /locus_tag="VFV93_07085"
     CDS             2308..4656
                     /locus_tag="VFV93_07085"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5165140.1"
                     /translation="MELRPNIIEERVTIAGRDLIFETGRIAGLADGAVTVRYGDSLVL
                     STAEGEAEPREGMDFFPLTVDYEERMYAAGKIPGGFIKREGRPSEHAILTARVTDRPL
                     RPLFPSGYRNEVQVMTVVLSTDQQNDPDILSINGASAALTISQVPFQGPVGAVRIGYV
                     NGELVVNPTQADLEESLMDVVVAGTDDAIMMVEGECKEVSEDVLVDGIALAHEQIKLL
                     VEAQNRLQQRVGKPKREVTVAPKDEAYATAMSDYLGARLNEAVFHADKETRIEATASL
                     KRETVAHFVDQATAAGQDAGAAEKKAKATFESLVKAAVRSAIIERGDRPDGRTPEEIR
                     PIWCEVGYLPRAHGSAIFTRGQTQALTVATLGSTKEDQMLDGLGLETSKRYMHHYNFP
                     PFSVGEVRRLRGPSRRDIGHGALAERALLAVLPEQTEFPYVLRVVSDIMASNGSSSMA
                     SVCGSTMALMDAGVPIKAPVAGVAMGLVTDESGRYRVLTDIQGLEDALGDMDFKVAGT
                     RTGVTAIQMDIKVRGITTQIMREALAQALTGRLFILDKMAETIAAPRDTQSEFAPSIV
                     TVKIAPDQIGAVIGPGGKVIRAIQESSNTKIDIEDDGTVYISGVGQEATDAAADQIRR
                     ITYTPSVGDRITGPVKTIIAVGAFVEIAPGKDAFVHISQLGTERVEKVEDAVQVGDEI
                     EVVVTAVRNDGKIDASRRAAITGEMPAPRQPREGGFGGPRRDGDRDRGPRRDGDRDRA
                     PRNDGPRNDGPRSDGQRQPERAMTDGRPSDSRRPGFIRRADN"
     gene            complement(4814..5335)
                     /locus_tag="VFV93_07090"
     CDS             complement(4814..5335)
                     /locus_tag="VFV93_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX5165141.1"
                     /translation="MSAQPDQSRRCPTCGNTVPAVARYCPNCGTRLNPAASYTADEFE
                     DALADVLDAPIEEPEAAPADPVVVPEPPSVTPEPEPPVTITPAVEPVEWTASASDWSD
                     PTSQPGTWSAQPPIATAPAKPRGNRTLWIILAIFGFLVFCCCGFIFVALLVDGTTSAV
                     EPDLSVIRSLFSA"
     gene            complement(5332..6381)
                     /locus_tag="VFV93_07095"
     CDS             complement(5332..6381)
                     /locus_tag="VFV93_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5165142.1"
                     /translation="MLEQLRRRMMIGILLGVVVVAGIVILSDAGALSRALREFDWRLL
                     PLVLTLTVFNYTLRFVKWQYYLRLLGVTTLPRRDSLLIFLAGFTMVMTPGKIGELLKS
                     YLVRLRSDVPMTRTAPVIFAERLSDGFALLVLSGFGLLVFRHGWQLLLIGAIASLIGL
                     VVIQRESWIHAILGRLERTRLGHGRGTAMIELYDSTRTLLQPKPLLTAIGIGVSSWLG
                     ECVAFFLVLVGLGIGASWNVLLASIFVFAASTWIGGASMLPGGLGAAELSVAGLLLLT
                     IDDPAMTSTLAGTATLSIRFATLWFGVLIGIVALSRVSGWTSTLQAPESEPPGPAHLT
                     MNGDRPQPHSTNGVR"
     gene            6659..7495
                     /gene="larE"
                     /locus_tag="VFV93_07100"
     CDS             6659..7495
                     /gene="larE"
                     /locus_tag="VFV93_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX5165143.1"
                     /translation="MSPETTQKYDELRRIIGEMESALVAYSAGVDSTLVLKVAHDMLG
                     DKVLAATGLSDTYPAEEMDEARALAAEIGAEHLHVNTMELTDPRYASNNSQRCFFCKT
                     ELYSKLVAVAKDRDFQWVLDGTNADDLGDHRPGLRAARDWNVRSPLVEAGMCKKEIRE
                     ISNELGLRTWDKPAFACLSSRFLYGDPITVEKLQKVAKAETAMRRLGFKGFRVRHHDN
                     VARIELEPDDMQRALDLREEINAGIIEAGYAFVTLDLGGYRAGNLNKLLKPKVIAAST
                     HQ"
     gene            complement(7568..8644)
                     /locus_tag="VFV93_07105"
     CDS             complement(7568..8644)
                     /locus_tag="VFV93_07105"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX5165144.1"
                     /translation="MSSTKFPVIFDGHNDTVLSLTGTGRSFFEKSAEGHIDLPRAKEG
                     GLGGGFFAVYIRDPQNFDDVTDPDEAAKQAMARYGDVSTMPSPMGLDHAQPEAMKLAA
                     ALYRVEKESQGQVKIVRTAAELQHCLDNGIFAMLLHFEGAEPIDTDLDALEVFYQAGF
                     RSLGLTWSRKNAFATGVPFKFPSSPDLGPGLTEAGKNLVRMCNELGVMVDLSHITEQG
                     FWDVQAISTKPLVATHSNAHVVSQSPRNLTDKQLDAVRDSGGVVGLNYHVGFLRPDGQ
                     HSDDTPLSVMVDHVDHLVEKLGIDGVALGSDFDGATMPAELKDAAGLPKLMAAMRDRG
                     YDDDSLRKIAHGNWVRVLKETWGE"
     gene            complement(8711..9886)
                     /locus_tag="VFV93_07110"
     CDS             complement(8711..9886)
                     /locus_tag="VFV93_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5165145.1"
                     /translation="MSIDIERARRETPGCETVLHFNNAGSSLMPAAVLDAQIEHLQLE
                     ARIGGYESADAAHDKVERMYDAAATLIGAGRDEIAAIENATRAWDMAFYSIPFESGDR
                     ILTAKAEYASNYIAYLQVAEKYGATVEVVPNDEHGQLSIEHLQAMIDDRVKLIAVTHV
                     PTNGGLVNPAAEIGEVARQAGVLYLLDACQSVGQLPVDVEAFGCDMLSTTGRKYLRGP
                     RGTGFLYVRRSILEQLEPVMLDLHAAQWTSRNTFTIRDDARRFENWETNYAAKIGLGV
                     ALDYALDWGIDSIWGRIQLLAGQLRQNLIAIPGVTVRDIGEVQGGIVTFTIEGRDLQE
                     VKDALSEQAINVTVSRTPSTRLDMEERELREVVRASVHYYNSEDEVSRFCAAIEAQA"
     gene            complement(9989..10585)
                     /locus_tag="VFV93_07115"
     CDS             complement(9989..10585)
                     /locus_tag="VFV93_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165146.1"
                     /translation="MRGKEVIFALLFTLVGAALATVGYFLLGDRVSSVVYYAALIIGL
                     GAALVTVRIGRIGPFEGELGSLRLEMDLRDRIMREADRAMRYDRDLSIIAIRQPGAEE
                     RWRSTVRNADDIIAFRKGLIILLLPETSRGGALMLLRRSMIESPAPIQAALVSAPLDG
                     RTGDELLDALLALVRESSQQGKIVMLGTDNIEALPIGA"
     gene            10762..>11390
                     /locus_tag="VFV93_07120"
     CDS             10762..>11390
                     /locus_tag="VFV93_07120"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HEX5165147.1"
                     /translation="MANEQGGNLNIDLLKRICETPGVAGREDAVRALVREEMAGLVDE
                     ISVDALGNVVGVRKGQGGPRVLVMAHLDEIGFLVKHIGDKGFIRIQPVGGFDPRVLPA
                     QRVIVHGFGGEQLRGALQISAKPIHLLDAGDIKPPKIDELFVDLGLSGDEVKARVEVG
                     DMVTLDRTVERVGNNVMGKSLDDRICVFQMIEALRRLSGPTQAEIVAVAT"
BASE COUNT         2132 a         3710 c         3600 g         1948 t
ORIGIN      
        1 ccagcgccga cggtccggcc accttcgcga atggcgaagc gcagcccctc ctcgatcgcc
       61 accggctgga tcagctccac ctgcatctgg atgttgtcgc ccggcatcac catctcgacc
      121 ccctcgggca actggatggc cccggtcaca tcggtcgtgc ggatgtagaa ctgcggccgg
      181 tagcccggga agaacggcgt gtgccggccg ccctcgtcct tgcccagcac gtacacctcg
      241 gccgcaaacg tcgtgtgcgg cttgatgctg cccggcttgg ccagcacctg cccgcgctcg
      301 acctcttccc ggtcgatccc gcgcaagagg cacccgacgt tgtccccggc caccccctcg
      361 tcgagcgtct tctggaacat ctcaacaccg gtcacgacga cactccgcgt gtccttgatg
      421 ccgatgatct cgatcgtctc accggtcttg acccggcccc gctcgacccg gcccgtcacg
      481 accgtcccac gccccttgat gccgaacacg tcctcgaccg gcatcaggaa cggctggtcg
      541 accgctcgct ccggcgtcgg gatgtagtca tcgaccgcct gcatcagctc ccagatcggg
      601 gcgtactccg gcgcactcgg gtcggtcgag gtgctctcca gcgccgccag cgcactgccg
      661 cggatgatgg ggatctcatc acccgggaag ttgtagttgc tgagcagctc gcgcacttcc
      721 agctcgacca gctcgagcag ttcctcgtcg tccatcatgt cgaccttgtt gaggaagacg
      781 accatggccg gcacttcgac ctggcgggcc agcaggatgt gctcacgcgt ctgcggcatc
      841 gggccgtccg gcgcactgac gaccaggatg gcgccgtcca tctgggccgc accggtgatc
      901 atgttcttga tgtagtcggc gtgccccggg cagtcgacgt gcgcgtagtg ccggttgtcg
      961 gtctcgtact cgacgtgcga gatggcgatc gtgatgccgc gctcacgctc ttccggcgcg
     1021 ttgtcgatcg agtcgaacga gcggaaggag gcttcgcccc gcaacgccag cgtcttggtg
     1081 atggccgccg tcaaggtcgt cttgccgtgg tcgacgtgtc cgatcgtccc cacgttgata
     1141 tgcggcttcg tccgctcgaa tctttgcttc gccacgctca accctcctgc gtacccaaga
     1201 tcccgaagtt accgatgccc tggcgcgatg ccttgtgatc ccaacgtgca cgtcggcgat
     1261 cgaccagaat tcaacaaaag ggcccatcta acgcaataga cgggccggtg gggagagagg
     1321 gattcgaacc cccgaagcat gacgcagctg gtttacagcc agcccccttt ggccacttgg
     1381 gtatctcccc ctggagccgg cggtgggact cgaacccgca acctgctgtt tacaaaacag
     1441 ctgctctgcc aattgagcta cgccggcaca cgcattcgaa ccccgggccg aaacccagcg
     1501 ctgaagtgta tagtctagcc ggttcgctgt caatccgttt tggcgcgggc ttttcgccgg
     1561 caagccgctg ccaatgacag attggcaatt gcggctatcg gccatttctg ttatggttat
     1621 gaggtgaatg cgtaacgtta cggcggggta gtttcaggcc gtagcgatga gggaggcaac
     1681 gcaccgtatg gtactgcaga agtcacagaa ggacgatatc gtcgagtcgt acaggaccca
     1741 tgataccgac acaggctcgc cggaagtcca gatcgcgctg ctgaccgagc gcatcaacgg
     1801 tctgatcgag catcttcgcg aacacgccca cgatcatcac tcgcgccgtg gcctgctgaa
     1861 gctggtcggc cgccggcgcc gcctgctcgc ctacctgagc gagaaggaca cggaccgcta
     1921 tcgtgccctg atcgcacggc tcggtctgcg ccgctaagca tcgccgcaag aagacgggtt
     1981 tggacatcat ccaacacgcc atcgccagga cagtccaacc aatcggctcg cttccctcag
     2041 gcccagggac agtttccctg ggcctctctg tgtccgcgtc ggacactgca tactcgggaa
     2101 ggagtcgcct gacgaacaat cgccattgat ccatcgcaat cagttgccgc cgggctgggg
     2161 gttgggaact gaagtgtgcc gacgcatgac ggcgtcacac tccagtccct gactctcaca
     2221 gcggcaccaa atctgcgcga cacgagtcgt cgcagccaga cgggacgcgc ccgcgtgtcc
     2281 caccgcatgt gaggaataca cacaccaatg gaactacgac ccaacatcat cgaagaacgc
     2341 gtcaccatcg cggggcgcga cttgatcttc gagaccggca ggatcgccgg cctcgccgac
     2401 ggcgccgtca ccgtcaggta cggcgatagc ctcgtcctct cgacggccga aggcgaagcc
     2461 gagccgcgtg agggcatgga cttcttcccg ctgacggtcg actacgagga gcgcatgtac
     2521 gccgccggca agatccccgg tggcttcatc aagcgcgaag gccgcccgag cgagcacgcg
     2581 atcctgactg cgcgcgtcac cgaccgcccg ctgcggccgc tcttcccgtc cggctaccgc
     2641 aacgaggtcc aggtcatgac ggtcgttctc tcgaccgatc agcagaacga cccggacatt
     2701 ctctcgatca atggcgcttc agccgcgctg acgatctccc aagtaccgtt ccaggggcca
     2761 gtcggcgccg ttcgcatcgg ctacgtcaac ggcgagctgg tcgtcaaccc gacccaggct
     2821 gacctcgaag agagcttgat ggatgtcgtc gtcgccggga cagacgacgc catcatgatg
     2881 gtcgagggcg agtgcaagga agtctccgag gacgtgctcg tcgacggcat cgcgctggca
     2941 cacgagcaga tcaagctgct ggtcgaggcg cagaatcgcc tccagcagcg cgtcggcaag
     3001 ccgaagcgcg aggtgacggt ggcgccgaag gacgaggcgt acgcaaccgc gatgagcgac
     3061 tacctcggcg ctcgcctcaa cgaggcagtc ttccacgcgg acaaggaaac ccgcatcgaa
     3121 gcgaccgcga gcctcaagcg cgaaacggtg gcccacttcg tcgatcaggc caccgcggct
     3181 ggtcaggacg cgggcgcggc cgagaagaag gccaaggcga cgttcgagtc actcgtcaag
     3241 gcagccgttc gcagcgcgat catcgaacgc ggcgaccgtc ccgatggccg gacgccggag
     3301 gagattcgcc cgatctggtg cgaggttggc tacctgccac gcgcgcacgg ttcggcgatc
     3361 ttcacccgtg gtcagacaca ggcgctgacg gtcgcgacgc tcggttcgac caaggaagat
     3421 cagatgctcg acggcctcgg ccttgagaca tccaagcgct atatgcacca ttacaacttc
     3481 ccgccgttca gcgtcggcga ggtgcggcgg ctgcgcggtc cgtcccggcg cgacatcggc
     3541 cacggcgcac tcgccgagcg agcgttgctg gcagtcctgc cggaacagac cgagttcccg
     3601 tacgtcctgc gggtcgtgtc agacatcatg gcgtcgaacg gttcgtcctc gatggccagc
     3661 gtctgcggta gcacgatggc gctgatggac gcgggggtgc cgatcaaggc gccggttgcc
     3721 ggcgtcgcga tggggctggt caccgacgag agcggccgct accgcgtcct gaccgacatt
     3781 cagggccttg aggatgcgct cggtgatatg gacttcaagg tcgccggtac gcgcacgggc
     3841 gtgaccgcga tccagatgga catcaaggtc cgtgggatca cgacccagat catgcgcgag
     3901 gcgctggcgc aggcgctgac tggccggctg ttcatcctcg acaaaatggc cgagacgatc
     3961 gccgcgccgc gcgacacaca gtcagagttc gcaccgagca tcgtcaccgt caagatcgcg
     4021 ccggatcaga tcggcgccgt catcggcccg ggtggcaagg tcatccgcgc catccaggag
     4081 tcgtccaaca ccaagatcga catcgaggac gacggcaccg tttacatcag cggtgtcggg
     4141 caggaggcga ctgacgcagc cgccgaccag atccgccgca tcacctacac gccgtctgtc
     4201 ggcgatcgca tcaccggccc ggtcaagacg atcatcgccg tcggcgcgtt cgtcgagatc
     4261 gcaccgggca aggacgcctt cgtccacatc tcgcaactcg gcaccgagcg ggtcgagaag
     4321 gtcgaggatg ccgtccaggt tggcgatgag atcgaggtcg tcgtgacggc cgtccgaaac
     4381 gacggcaaga tcgacgcatc ccgccgggca gccatcaccg gcgagatgcc ggccccgcgc
     4441 cagccgcgcg agggtggctt cggcggcccg cgccgcgacg gcgaccgcga tcgcggccca
     4501 cgtcgcgacg gcgaccggga ccgtgctccg cgcaacgacg gcccacgcaa cgacggccca
     4561 cgcagcgacg gccagcgcca gcctgagcga gcgatgaccg acggccggcc tagtgactcc
     4621 cgccgccccg gcttcatccg ccgcgccgac aactaaccgc cgcgagacac agatgaacca
     4681 aggcaccctg gacgagattg ttcagggtgc cttgtcgttc tgagccgccg cgaaggatct
     4741 ctccgagtaa gcctcgtgta gcgtctatgc ggtgagttcc ttcgcggcgg ctcagaacga
     4801 caggagacct gctctacgcg gagaatagtg atcgaatgac tgatagatca ggctcgacag
     4861 cgcttgttgt gccatcgacc aacagcgcaa cgaagatgaa tccgcagcag cagaagacca
     4921 gaaatccaaa gattgcgagg atgatccaga gagtcctgtt tccccgtggc ttagccggcg
     4981 cggttgcgat cggcggttgc gcgctccatg ttcccggctg gctggtcgga tcgctccagt
     5041 cgctggcgct ggcggtccat tcaactggtt caacggctgg tgtgattgtg accggtggtt
     5101 caggctccgg cgtcacggag ggtggttcag gaacgacgac gggatcagcc ggggcagcct
     5161 ctggctcttc gattggagcg tcgaggacgt cggcaagcgc gtcttcgaac tcgtcagcgg
     5221 tgtaggaggc agcgggattg aggcgtgtcc cgcagttcgg gcagtagcga gccacggcgg
     5281 gaaccgtgtt gccacacgtc gggcatcgtc gtgactgatc cggttgcgcg ctcatcgtac
     5341 cccgttcgtc gaatgtggtt gcggccggtc gccgttcatc gttaggtggg ctgggccggg
     5401 cggctcggac tctggcgcct gaagggtact cgtccagccg gaaacgcgcg agagggcaac
     5461 gatcccgatc aggacgccga accagagcgt cgcaaagcga atcgacaggg tcgcggttcc
     5521 ggcgagcgtc gaggtcatcg ctggatcgtc gatcgtgagc agcagcaggc cggcgaccga
     5581 gagctcggcg gcgccaagcc cacccggcag catcgaggcg ccgccgatcc aggtcgaggc
     5641 cgcgaagacg aagatcgacg ccagcagaac gttccaactg gcaccgatgc cgagcccgac
     5701 cagcaccagg aagaaggcga cgcactcacc cagccacgaa ctgaccccga ttccaatcgc
     5761 tgtcagtagc ggcttcggtt gcaggagggt acgagtgctg tcgtacagct cgatcatcgc
     5821 cgtaccacga ccgtggccga gcctcgtccg ttccagacgg cccaggattg cgtggatcca
     5881 cgactcgcgc tggatgacga caagcccgat caggctcgcg atcgcgccga tcagcagcag
     5941 ttgccagccg tgacggaaca cgaggagtcc aaatcccgaa aggaccagca gcgcgaagcc
     6001 gtccgagagg cgttcggcga agatgaccgg cgccgtccgc gtcattggca catcggaccg
     6061 caggcggacg agatatgact tcagcagctc gccgatctta cccggcgtca ttaccatcgt
     6121 aaaaccggca aggaagatca ataaagaatc ccgccggggg agcgtcgtga cgccgagcaa
     6181 gcgcaggtag tactgccact tgacgaagcg cagggtgtag ttgaagaccg tcaacgtcag
     6241 cacgagcggc aacaagcgcc agtcaaattc gcgcagcgcc cgcgagagcg ctccggcgtc
     6301 gctcagaatg acgatgccgg caacgacgac gacgcccagc aggataccga tcatcattcg
     6361 ccggcggagt tgttcgagca ttccagacgc tttccatcgc tacgggtgta ttggtcggac
     6421 gcaggcggca gcggctgaga ctatgccaaa cgaatgccac caccggccgc gtcagcgctt
     6481 cgtcccgtgc aatctgtctc cagcgaccgt ttgacctgct tgtggtctcg cggctctccc
     6541 tgtcaggaca acggttcaca tcgcatgtgg ttatgttgtg tcgttgacat tgctgccgac
     6601 gctccggtag catcccagat ggctgccagc cggtatcgac cattgaaagg cgtgacccgt
     6661 gtctcccgag actacccaga aatatgacga actgcgccgg atcatcggcg agatggaatc
     6721 ggcgctggtc gcctacagtg ccggagtcga ctcgacgctg gtgctcaagg tcgcccacga
     6781 catgctcggc gacaaagtcc tggccgcaac cggcctgtcg gacacctacc ccgccgaaga
     6841 gatggacgag gcccgcgcgc tggcagccga gatcggagcc gaacacctgc atgtcaacac
     6901 gatggagctg accgatccac gctacgccag caacaacagc cagcgctgct tcttctgcaa
     6961 gacggagctg tattcgaagc tggtcgccgt cgccaaggat cgcgacttcc agtgggtgct
     7021 cgacggcacc aacgccgacg acctgggcga ccaccggccc ggcctgcggg ctgcccgcga
     7081 ctggaacgtc cgtagcccgc tcgtcgaagc cggcatgtgc aagaaggaga tccgcgagat
     7141 ctcgaacgag cttgggttgc gcacctggga caagcccgcc ttcgcctgcc tctcctcacg
     7201 ctttctctac ggcgatccga tcactgtcga gaaactccag aaggtcgcca aggccgaaac
     7261 ggcgatgcgc cggcttggtt tcaagggctt ccgcgtgcga catcatgaca acgtcgcccg
     7321 gatcgagctc gaacctgacg acatgcaacg cgcactcgac ctgcgcgagg agatcaacgc
     7381 cggcatcatc gaagccggct acgccttcgt caccctcgac ctcggcggct accgggccgg
     7441 caacctcaac aagctcctca agccgaaggt catcgcggca tcgactcatc agtagttcgc
     7501 acgtcgaccg caactggcag gggcgaggca gtgcctcgcc cctaccggtg gtgtatgccg
     7561 acgcgagtta ttccccccag gtctccttca agacccgcac ccagttgccg tgggcgatct
     7621 tgcggagtga gtcgtcgtcg tagccgcggt cgcgcatggc ggccatcagc ttcggcaggc
     7681 cggcggcgtc cttgagctcg gccggcatcg tcgcgccatc gaagtctgag ccaagcgcaa
     7741 cgccgtcgat gccaagcttc tcgaccaggt gatcgacatg atcgaccatg accgacagcg
     7801 gcgtgtcgtc gctgtgctgg ccatccggcc gcaggaagcc gacgtgatag ttgagaccga
     7861 cgacgccgcc gctatcgcga acggcgtcga gctgcttgtc ggtcaggttg cgcgggctct
     7921 ggctgacgac gtgcgcgttc gagtgcgttg ccaccagcgg cttggtcgag atcgcctgga
     7981 cgtcccagaa cccctgctcg gtgatgtgcg acagatcgac catgacgcca agttcgttgc
     8041 acatgcgaac gaggttcttg cccgcctcgg tcaggccggg gccgagatcg ggcgagctgg
     8101 ggaacttgaa cggcacgccg gtcgcgaagg cgttcttgcg gctccaggtc agacccagcg
     8161 aacggaagcc ggcctggtag aagacctcca gcgcgtccag atcggtgtcg atcggctcgg
     8221 cgccctcgaa gtggagcagc atcgcgaaga tgccgttatc gaggcagtgc tgcaactcgg
     8281 ccgccgtccg aacgatcttg acctgaccct gcgactcctt ctcgaccctg tagagcgccg
     8341 ctgccagctt catcgcttcc ggctgggcat ggtcgaggcc catcggtgat ggcatcgtgc
     8401 tgacgtcgcc gtagcgggcc atcgcctgct tggcggcttc gtcgggatcg gtcacgtcgt
     8461 caaagttctg cggatcgcgg atgtagacgg cgaagaaacc gccgccgagc ccgccctctt
     8521 tggcgcgcgg caggtcgatg tgaccctcgg ccgatttttc gaagaacgag cggccggtgc
     8581 cggtcagact caggaccgtg tcgttatgac cgtcgaagat aactgggaac ttcgtgctgc
     8641 tcaccgtggt cttcctctcc ctcgtacgca tgtcacgtcg cgccattgtc gcgcgaggtg
     8701 gcgccgggta tcaagcctgc gcttcgatcg cggcgcagaa gcggctcacc tcatcttccg
     8761 agttgtagta gtggaccgag gcgcgcacga cttcgcgcag ctcccgttct tccatgtcca
     8821 gccgagtcga tggtgttcgc gaaacggtga cgttgatggc ctgttccgac agcgcatctt
     8881 tgacctcctg caggtcacga ccctcgatcg tgaaggtgac gatgccgccc tgcacctcgc
     8941 cgatatcgcg aacggtcacg ccgggaattg cgatcaggtt ctgccggagc tggccggcga
     9001 gtagttgaat ccgcccccag atcgagtcga tgccccaatc gagcgcgtag tccaaagcaa
     9061 cgccgaggcc gatcttggcg gcatagttcg tctcccagtt ctcaaaccga cgggcgtcgt
     9121 cgcggatcgt gaacgtgttg cgggaggtcc actgagccgc atgcagatcg agcatgaccg
     9181 gttcaagctg ttcgaggatg ctgcggcgga cgtagaggaa gccggtccca cgtgggccgc
     9241 gcagatactt gcgccccgtc gtcgagagca tgtcgcagcc aaatgcttcg acatcgaccg
     9301 gtaactgacc gaccgattgg caggcgtcca gcaggtagag gacaccggcc tgcctggcga
     9361 cctcgccgat ctcggcggcc ggattgacca gcccgccgtt agtcggaacg tgggtgaccg
     9421 cgatcagctt gacgcgatcg tcgatcatcg cctgcagatg ctcgatcgag agttgcccgt
     9481 gctcgtcgtt gggaacgacc tcgaccgtcg caccgtactt ctcggcgacc tgcaggtagg
     9541 cgatgtagtt gctggcgtac tccgccttgg ccgttagtat cctgtctccg gactcgaacg
     9601 gaatcgaata gaacgccatg tcccaggcac gggtcgcgtt ctcaatcgcg gcgatctcgt
     9661 cccgtccggc gccgatcagc gtggccgccg cgtcgtacat gcgctcgacc ttgtcgtggg
     9721 ccgcgtcagc cgattcgtaa ccgccgatcc gcgcttcgag ctgcaagtgc tcgatctggg
     9781 cgtcgagaac ggcggcaggc atcaacgacg agccggcgtt gttgaagtgc agcaccgtct
     9841 cacagcccgg cgtctcgcgt cgcgcacgct cgatatcgat cgacatcgtc aattcccctc
     9901 tgtcgcagcc acggaagtgc atcgatgccc ggcaggattc gatcctgccg ggcatcgcgc
     9961 attctagccg tgattgtgca aaggtcgatc aggctccgat tgggagcgcc tcaatgttgt
    10021 cggtgccaag catgacgatc ttgccctgtt gtgaggattc gcgaaccagc gccaggaggg
    10081 catcgagcag ttcgtcgccg gtccggccgt ccagcggggc gctgacgagc gcggcctgga
    10141 tcggagcggg cgactcgatc atcgagcggc gcagtaacat cagagcgcca ccgcgtgacg
    10201 tttccggcag aagcagaatg atcaggccct tgcggaaggc gatgatgtca tcggcgttgc
    10261 ggacggttga gcgccagcgc tcttccgcgc caggttgacg gatcgcgatg atactgaggt
    10321 cacggtcgta acgcatcgcg cgatccgcct cgcgcataat gcggtcgcgc agatccatct
    10381 ccagccgcag cgatcccaat tcgccctcga atggaccgat ccgcccgatg cggaccgtca
    10441 ccagtgccgc gccaagtcca ataatcagag ccgcataata gacaacacta ctgactcgat
    10501 cgccaagcag aaaatagccg acagtcgcca acgcagcgcc gaccagcgta aacagcagcg
    10561 cgaaaatgac ctctttgcct cgcatccgca gcaccccctg cacccggaga tcggtgactc
    10621 gtcgcagccc aactgacttt gaccgtctct ggagtccctg ctagagtctt gcagcattgt
    10681 gggaggctga aaagagaccg aaagtaccgc aagtgaggcg tactatcagg caaatcgtat
    10741 acgaaagggt ggggacacga ggtggccaac gaacagggcg gcaatctgaa catcgatctg
    10801 ctcaagcgaa tctgcgagac acctggtgtg gcaggccgcg aggatgccgt ccgggcgctg
    10861 gtgcgcgagg agatggccgg gctggtcgac gagatctcgg tcgatgcgct cggaaacgtc
    10921 gtcggggtcc gcaaggggca gggcgggccg cgcgtcctcg tgatggcgca cctggacgag
    10981 atcggatttc tcgtcaagca tatcggcgac aagggcttca tccgcatcca gccggtcggc
    11041 gggttcgatc cgcgcgtgct gccggcccag cgcgtcatcg tccacggctt cggcggcgag
    11101 caactgcggg gcgcgctgca gatcagcgcc aagccgatcc atctgcttga cgccggcgac
    11161 atcaagccac ccaagatcga cgagttgttt gtcgacctcg ggctgagcgg tgatgaggtc
    11221 aaggctcgcg tggaggtcgg cgacatggtc acgctcgatc gaaccgtcga gcgcgtcggc
    11281 aacaacgtga tgggcaagtc gctggacgat cgcatctgcg tcttccagat gatcgaggcg
    11341 ctgcgacgcc tctccggccc gactcaggct gagatcgtcg ccgtcgcaac
//
LOCUS       DASUGJ010000317         6979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_602558, whole genome shotgun sequence.
ACCESSION   DASUGJ010000317 DASUGJ010000000
VERSION     DASUGJ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602558"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..2225)
                     /locus_tag="VFV93_07125"
     CDS             complement(120..2225)
                     /locus_tag="VFV93_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5165148.1"
                     /translation="MLPDTASSAHPPRLADRLAAARRGRFVGRHAELDTFRSALLAPA
                     PPFAVLYLYGPGGIGKSSLLREYARIASEVDKPAFRIDGRNVDPSPPGFLRAVRHALQ
                     LDDGAPSVIEDWPPGSVLLIDTYEMLESLDEWLREVFLQQLPAETIVVIAGRNPPAAA
                     WLADLDWSALTRTLPVRNLLPEESETYLATRGVPGDQHGSVLAFTHGHPLALALVADV
                     LQRGNHLDGFDPKDEPDLLRLLLEHLVQDVPSPLHRRALEVSVMAWSTTEELLARVLD
                     VDDAHELFEWMRRLSFIEHGAFGLFPHDLAREVLDEDLRWRSPTGRRELSIRLATCLH
                     DWFHQSRGAEQQRVWFDLLYLYRHNPAFRPYFDWSAIGTAYAEPALPADHPAIEAMVL
                     HHYGVESAAIARHWLARQPEAFLVYRDAGGDLFGFMAHLELQRATAEDIAADPATTAA
                     LEYVQRHRPARPDEVIAYLRFWMSREAEQGISPALNLTAINASIYWTTRQKLAWNFIA
                     LADPAFLQPHFTSIHMWRVPEADFEVGGRRFGIFAHDWRVETPAEWLQIKASLASDTA
                     VATDARPSAPASVILSDEQHREAVRQALRDFCRPDLLAGNPLVGTWLVHGAAHDGAQG
                     AALQSLLREAVETLASPKDLKFHRAIWHTYIEPAPTQELAAELLNLPFNTYRYHLARG
                     IERIDAWLRQRGALGGDTI"
     gene            complement(2468..5071)
                     /locus_tag="VFV93_07130"
     CDS             complement(2468..5071)
                     /locus_tag="VFV93_07130"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX5165149.1"
                     /translation="MNVEQVATSDGGDEQPYVFISYASVDRERVLPLVDRLERAGIRT
                     WIDREGILGGTNYAREIVEALRGSAALLIMCSRASFASQNVRQEIALAWKHQRPYLPL
                     LLEPVSIPADLEYWLEGTQWIEVVDDGADRWLERSVAAIGRIVSGTRSAAPIAEVDAD
                     PALRPVGLPEAPTRLLGRETDLAPLLDLVRRADVRLVTLTGAGGIGKTRLALEVGRLL
                     TEEFPDGVAFVDLASITDTRQVGPAIATGLGLRPVGNQPPEALLSTALGSLRILLILD
                     NFEQLLDATPLVATVLGSTRSLKILVTSRAPLHLRAEHEYPVSPLQLPPDEEIPLADL
                     ADNGAVALFTDRAEAAKPGFLLDEQNAQFIAEICRRLDGIPLAIELAAARIRMLPPPA
                     LLRRLEHRLPLLAGGARDLPARQQTLRATIDWSYGLLSPEQQRLFRYLAVFAGGSTLE
                     AAEFVAGEPGGQDVLDLLTVLIDQSLVQQRDGADDEPHFTMLETIREYALEQLADSDD
                     VTVAGSRHADYFAQLADRDPRRLRVDEKNLWIDQLDRDAANLSAALGWALDQHDDAPA
                     VRISLALAELQWARARVSEARNQVAAVLERVEIDEGSDAHTRLLEWAISFARIAGDLE
                     QARAYCMQALEVARRIGEPAHTALMLQNLGVLSWRAGDLLTAQQLLEEALAMQQAQGD
                     DRASADTLMELAMTEYLRGDHDHALTHVEQSIEMQRSYDPSAQFAATLQPYAWILIDQ
                     GKLAMAAECLREGMSVFRLVGMRTGLIWTTGGVARLAAANGDATVAARLAGAAATLRS
                     KMHVPLPNVVQQNLEEHLRISRDQIGETRYQECWDDGAHLSIDEMSAAALAYLDSASA
                     SYTFTPSTSER"
     gene            complement(5164..5943)
                     /locus_tag="VFV93_07135"
     CDS             complement(5164..5943)
                     /locus_tag="VFV93_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5165150.1"
                     /translation="MAQEERRDLVLANLSWPEVAEIKDQVELVLQPVGSNEQHGPNLA
                     LCMDIASSFEFCRRVSERLYPKVLVAPPVPWGVSFHHMNFPGTITLSNETFVDVLTEI
                     VASLHHHGFKRFMFMNGHGGNIPALGVATVRVKEEIDPAFIGAGSYFHFMDREIHKTF
                     GATPYTGHACEAETARAMELIPQHVKYDTIAKGDLTDLAYEFNQTLKKYSVTVPYRFD
                     EITRNGALGDARIATPEYGKALVDSALNNFTAFVEELIANS"
     gene            complement(6013..6867)
                     /locus_tag="VFV93_07140"
     CDS             complement(6013..6867)
                     /locus_tag="VFV93_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5165151.1"
                     /translation="MLIDINGTNVHVVREGSGTPLVIPSLAGTPIYERTFSPRLREYL
                     TLVFVELRANRADPGEPDLTLDRVLDDLDAVRATLGYDRISVLGHSAHSILALAYAAQ
                     YPSHTDRVVVVGGPASTRPSTEMDMLHEAYWDTVASPERKQFQADLEAAYDPTLPRTP
                     SEALIAQYVTNGPRTWYDPSFDCTPLWEGHDNLDLDLIMRFWGPDGMFQRFDSEVGLP
                     GISCPVLIAMGAFDFLCPPILWAGLHQRLAHHTFHTFERSGHLPHVDEQDHFDEVLLK
                     WMGVDPAG"
BASE COUNT         1159 a         2191 c         2407 g         1222 t
ORIGIN      
        1 ggacctcccc tgcgttagcc acacgggact gtaaagcagg agaggtcctt cgctgccggg
       61 tgccctctgg gcggtggacg acagggagac tgcccttttc ttattggaga gtaggatgtt
      121 cagatcgtgt ccccacccag cgcgccccgc tgtcggagcc aggcgtcgat gcgctcgatg
      181 ccgcgggcga ggtggtatcg ataggtgttg aagggcaggt tgagcagctc ggcggcgagt
      241 tcctgggtcg gggcgggctc gatgtaggtg tgccagatgg cgcggtggaa cttcaggtcc
      301 ttcggtgacg ccagtgtctc aacggcctcg cggagcagcg actggagtgc agcaccctgc
      361 gcaccgtcgt gcgctgcgcc gtggaccagc catgtcccga ccagcgggtt gccggcgagc
      421 aggtcgggac ggcagaaatc gcgcagcgct tgccggacgg cctctctgtg ttgttcgtcg
      481 gagaggatca cgctcgcagg cgcggatggt cgggcgtcag tggcgacggc ggtgtccgag
      541 gcgagcgacg ccttgatctg cagccactcc gccggcgttt ccacccgcca gtcgtgcgcg
      601 aagatcccga agcgccgccc gccgacctcg aagtcagcct ccggaactct ccacatgtgg
      661 atgctcgtga agtgcggctg caggaaagcc gggtcggcaa gcgcgatgaa gttccaggcc
      721 agcttctggc gcgtcgtcca gtagattgat gcgttgatcg ccgtcaggtt gagtgccggc
      781 gagatcccct gttccgcctc ccggctcatc cagaagcgca ggtaggcgat cacctcatcg
      841 ggccgcgccg gccgatggcg ctggacgtac tccagcgcgg ccgtcgtcgc cgggtcggcc
      901 gcgatgtctt cggcggtcgc gcgctgcaac tccaggtggg ccatgaagcc gaacagatcg
      961 ccgccggcgt cgcggtagac gaggaatgcc tccggctggc gcgcgagcca gtggcgcgcg
     1021 atggcggccg attcgacgcc gtagtggtgc agcaccatcg cctcgatcgc gggatggtcg
     1081 gcgggcagcg ccggttccgc gtaggcagtg ccgatcgctg accagtcgaa gtagggccgg
     1141 aaggcgggat tgtggcgata gaggtagagc agatcgaacc agacgcgctg ctgttcggcg
     1201 ccgcgcgact gatgaaacca gtcgtgcagg caggtcgcga ggcgaatcga cagctcgcgg
     1261 cggccggtcg ggctgcgcca gcgcaggtcc tcatcgagca cctcgcgcgc cagatcgtgc
     1321 gggaagaggc cgaacgcccc atgctcgatg aacgacagac gccgcatcca ctcgaacagc
     1381 tcgtgggcgt cgtcgacatc gagcacccgc gccagcagct cttcggttgt cgaccaggcc
     1441 atgaccgaca cctccagcgc gcggcgatgg agcgggctcg gtacgtcctg gacgagatgc
     1501 tccagcagca gccgcaggag atccggctcg tccttcgggt cgaacccgtc gaggtgattg
     1561 ccacgctgga ggacatcggc gaccagcgcc agcgccaagg ggtgaccgtg ggtgaaggcc
     1621 agcacgctgc cgtgctggtc gcccggcacg ccgcgcgtgg cgaggtaggt ctcgctctcc
     1681 tcgggcagca ggttgcggac cggcagtgtt cgcgtcagcg ctgaccagtc gaggtcagcc
     1741 agccaggccg ccgccggcgg attacggccg gcgatgacga cgatcgtctc ggccggcagt
     1801 tgttgcagga acacctcgcg cagccactcg tccagcgact caagcatctc gtaggtgtcg
     1861 atcagcagca cgctgccggg cggccagtcc tcaatgacgg agggtgcgcc gtcgtccagt
     1921 tgcagggcat gccgcaccgc gcgcaggaag ccgggcggcg agggatcgac gttgcggccg
     1981 tcgatgcgga aggccggctt gtcaacctcg ctcgcgatcc gcgcgtactc gcgcagcagg
     2041 ctggatttcc cgatcccacc cgggccgtag aggtacagga cagcgaatgg tggtgcaggc
     2101 gccagcagcg cggagcggaa ggtgtccagc tctgcatgcc ggccaacgaa gcgaccgcgt
     2161 cgcgcggcgg ccagtcgatc ggcgaggcga ggtgggtgtg cggatgacgc agtatctggg
     2221 agcatcggca tcgacctctg gcgtcagcga caatgcaacc gattcgagtg ctcgcgaaca
     2281 cagccgacaa tgtgatcggc tcatctgcgt tcaggtgtgc agtgctgcta ccccacgacc
     2341 agcgatgccg cattgtcgca catggagccg ttcgccgtct ggttccctgt agcatgggcc
     2401 ggttcgacgg acctgatcca tgcgactgcc ggcagtcgcg gtcgtgacga gtgcatgacc
     2461 ggactgctca tcgctccgac gtagacggtg tgaacgtgta gctggcactg gcactgtcga
     2521 gataggccaa cgccgctgcc gacatttcat cgatcgacag gtgcgctcca tcatcccagc
     2581 actcctgata gcgtgtttcc ccgatctggt cgcgactgat cctgagatgc tcttccaggt
     2641 tttgctggac cacattcggc agcggcacat gcatcttcga gcgcagggtg gcggccgcgc
     2701 cggccaggcg agccgcgacc gtcgcgtcgc cgttggcggc ggccagccgg gcgacgcctc
     2761 ccgtcgtcca gatgaggccc gttcgcatgc cgacgaggcg aaagacagac atcccctctc
     2821 gcaggcactc cgcggccatc gcgagctttc cctggtcgat gagaatccag gcatacggtt
     2881 ggagggttgc cgcgaattgc gcgctcgggt cgtacgatcg ttgcatctca atgctctgct
     2941 cgacatgcgt gagcgcatga tcgtggtcgc cgcgtagata ttcggtcatc gccaactcca
     3001 tcagcgtgtc agcactggcc cgatcgtcac cctgcgcttg ctgcatcgcg agcgcctctt
     3061 cgagcagctg ctgcgccgtt agcagatctc cggcgcgcca actgagaacg ccgaggtttt
     3121 gcagcatcaa cgcagtgtgt gccggctcac cgatccgccg cgcgacctcg agcgcttgca
     3181 tgcagtacgc gcgtgcctgc tcaagatcgc cggcgatcct cgcgaacgag atggcccatt
     3241 cgaggagccg ggtatgcgca tcggaacctt cgtcgatctc gacacgttca aggacggccg
     3301 cgacctgatt gcgcgcctcg ctgacccgcg cacgggccca ttgcagctcc gcaagggcga
     3361 gtgagatccg aaccgccggg gcatcatcat gctggtcgag cgcccagccc aacgcggcgg
     3421 acaggttcgc ggcgtcccgg tcgagttggt cgatccagag gttcttttcg tcaacgcgga
     3481 gacgtctggg gtcgcggtcg gcgagttgcg cgaagtagtc ggcgtggcgg ctgccggcca
     3541 cggtcacgtc gtcactgtcg gcgagctgct ccagcgcgta ctcacggatc gtttcgagca
     3601 ttgtgaaatg cggttcgtcg tcggcgccgt cacgttgctg gacgaggctc tggtcgatca
     3661 gcaccgtcag cagatccagg acatcctggc cacccggctc gcccgcgacg aactcggcag
     3721 cctccagcgt cgaaccgccg gcgaagacgg cgagatagcg gaacagtcgc tgctgctcgg
     3781 gggagagcag gccgtagctc cagtcgatcg tggcgcgcag ggtctgctgc cgggcaggca
     3841 ggtcacgcgc gccgccggcg agcagcggca ggcgatgttc gagccggcgc aggagtgctg
     3901 gcgggggcag catccggatg cgtgccgccg cgagctcgat cgcgagcggg atgccgtcca
     3961 accggcggca gatctcggca atgaactggg cattctgctc atcgagcagg aaccctggct
     4021 tcgctgcctc ggcacggtcg gtgaagagcg cgacggcgcc gttgtctgcc agatccgcga
     4081 gcggtatctc ctcgtctggt gggagttgta gcggtgacac cggatactcg tgctcggctc
     4141 ggagatggag cggggcacgg ctcgtcacga ggatcttcag cgatcgtgtc gacccaagca
     4201 cggttgcgac gaggggtgtg gcatccagga gttgctcgaa gttgtcgagg atgagcagga
     4261 tccgcaggct cccgagcgcc gttgagagca gcgcttccgg cggctgatta ccgactggcc
     4321 gcagtccgag cccagtcgcg atggcgggtc cgacctgccg ggtgtcggtg atcgacgcca
     4381 gatcgacgaa ggcgacgccg tccggaaact cctcagtcag gagccggcca acttcgaggg
     4441 ccaggcgcgt cttgccaatt cccccggcgc cggtcaacgt gacgagccgg acgtctgccc
     4501 ggcgaacgag gtccagcagt ggcgccaggt cagtctcccg gccgaggagt cgcgtcggcg
     4561 cttctgggag cccgacgggg cgcagtgccg gatcggcgtc gacctcagcg atgggggcag
     4621 ccgagcgcgt gccggagacg atgcgtccga tcgccgcgac gctgcgctcg agccatcgat
     4681 cggcgccgtc gtcgacgacc tcgatccatt gcgtgccttc gagccagtac tccaggtcgg
     4741 cagggatgct gaccggttcc agcagcagcg gcaggtaggg ccgttgatgc ttccaggcga
     4801 gcgcgatctc ctgcctgaca ttctgggacg cgaacgacgc tcgcgagcac atgatgagca
     4861 gggccgccga gcccctcagg gcctcgacga tctcgcgggc gtagttggtt ccgccgagaa
     4921 tcccctcgcg gtcgatccac gtccggatgc cggcgcgttc aagtcggtcg accagcggca
     4981 gcacccgctc ccgatccacg ctggcatagc tgatgaagac gtacggctgc tcgtcgcctc
     5041 cgtcggatgt cgctacttgt tcgacgttca tggatgctca ccaccgctag cagcatacga
     5101 gcatcgcgac gcatgcgcaa gcgttgcctg gcgcccggcc gacgaacgga ttgtgtgcgt
     5161 gtctcagcta ttcgcgatca gctcctcgac gaaggcggtg aagttgttga gtgcgctgtc
     5221 gacgagcgcc ttgccgtact caggcgtggc gatccgggcg tcgccgaggg cgccattgcg
     5281 ggtgatctcg tcgaagcggt aggggactgt cacgctgtac ttcttcaagg tctggttgaa
     5341 ctcgtaggcc aggtcggtca ggtcgccctt ggcgatcgtg tcgtacttga cgtgctgcgg
     5401 gatcagctcc atcgcgcggg ccgtctcggc ctcgcaggcg tggccggtgt agggcgtcgc
     5461 gccgaacgtc ttgtggatct cgcggtccat gaagtggaag tagctgccgg cgccgatgaa
     5521 ggccgggtcg atctcctcct tgacgcggac ggtcgcgacg ccgagggccg ggatgttgcc
     5581 gccgtggccg ttcatgaaca tgaagcgctt gaagccgtgg tggtggagcg aggccacgat
     5641 ctcggtcagg acgtcgacga acgtctcgtt cgagagcgtg atcgtgccgg ggaagttcat
     5701 gtggtggaac gagacgcccc acggcaccgg cggcgcgacg agcaccttcg gatagagccg
     5761 ttcgctgacg cggcggcaga actcgaacga ggaggcgatg tccatgcaga gcgccagatt
     5821 cgggccatgc tgctcgttcg atccgaccgg ctgcaggacg agctcgacct ggtccttgat
     5881 ctcggccacc tccggccacg acagattcgc cagcacgaga tcgcgtcgtt cttcctgtgc
     5941 catgtgcttc ctccctctcg atgactcact gaccggcctg gatgctcgcg accgggctgg
     6001 cgcgacgctg gatcagccag ctggatccac gcccatccac tttagcagca cctcatcgaa
     6061 gtggtcctgc tcgtcaacgt gcgggagatg tccgctccgc tcgaaggtgt ggaaggtgtg
     6121 gtgtgccagt cgctgatgca gccctgccca caggatgggc gggcagagaa agtcgaacgc
     6181 gcccatcgcg atgaggacgg ggcagctgat gcccggcaag ccgacttccg aatcaaagcg
     6241 ctggaacatg ccgtccggcc cccagaaccg catgatgaga tccagatcga ggttgtcgtg
     6301 cccttcccag agcggcgtgc aatcgaagga tgggtcgtac caggtgcgtg gaccattcgt
     6361 gacgtactgc gcgatcagcg cttcactcgg cgtgcgcggc agtgtcgggt cataggccgc
     6421 ctcgagatcg gcctggaact gcttgcgctc cggtgatgcg acggtgtccc agtaggcctc
     6481 gtgaagcatg tccatctcag tcgacggccg ggttgatgct ggtccaccaa cgaccacgac
     6541 tcgatccgta tgcgacgggt actgtgcggc gtaggcgagg gccagaatgc tgtgggccga
     6601 atgccccaga acggagatac ggtcgtatcc gagcgttgcg cgaacggcgt ccagatcatc
     6661 gagcacgcga tccagcgtca gatcaggttc tcctggatcg gcgcgattgg cgcgcaattc
     6721 gacgaagacc agtgtgaggt actcgcgtag acgcggcgag aacgttcgct cgtagatcgg
     6781 tgtgccagcc aggctgggaa tgaccaaagg tgttccgctg ccttcgcgta cgacgtgaac
     6841 gttggtaccg ttgatgtcga tcagcactgg gtctaccctt tcgatcaatt gactcgactt
     6901 tgactgttac ggcgccatgc cgctgacgac gatgacagga cctgtttgct cgatacgccc
     6961 attctgggcg atgctgctg
//
LOCUS       DASUGJ010000318         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1853796, whole genome shotgun sequence.
ACCESSION   DASUGJ010000318 DASUGJ010000000
VERSION     DASUGJ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853796"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VFV93_07145"
     CDS             <1..1154
                     /locus_tag="VFV93_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165152.1"
                     /translation="RNFGWSQMEGQHCFAADCDASLYTLPIAEYSHDDGCSVTGGYVY
                     RGSDYPALAGRYFFGDYCSGKIWSLTPDGDGWTMTPELDTDSWITSFGEDEAGELYLV
                     DLAGVLYRVTTSELQSPSIDNPAMLRTWQRTDKPVADLTVDRTWMWGPEPFTNAIPEP
                     YAESPDGSRMVQYFDKARMEITQPDAEDDGIWYVTNGLLVVEMMSGQIQTGDNRFESQ
                     QPATENVAGDPGVESGPTYATLAPLRGAPPAADSATLTQRIAPDGTVSDDPTMASFGI
                     TAAQRVQVPHIDHQIASVFWEFMNSTATVYEDGELVNDLLFVNPYYATGYPITEAYWT
                     TILLDSNETDVLLQCFERRCLTYTPGNEPGWQVEAGNVGQHYYRWRYGS"
     gene            complement(1399..1872)
                     /gene="purE"
                     /locus_tag="VFV93_07150"
     CDS             complement(1399..1872)
                     /gene="purE"
                     /locus_tag="VFV93_07150"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX5165153.1"
                     /translation="MSSSDKPRVVIVMGSESDRPFMTEAEKMLDELGIPSTTVVMSAH
                     RTPAKVQEMASTARDRGVEVIIAGAGWAAALPGTIAAWTTLPVIGVPLATSDLNGLDA
                     LLAIVQMPPGIPVATVAIGKGGARNAAILAAQIIATGDEKVRASVEEHRQRLAGK"
     gene            complement(1865..>2404)
                     /gene="larC"
                     /locus_tag="VFV93_07155"
     CDS             complement(1865..>2404)
                     /gene="larC"
                     /locus_tag="VFV93_07155"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HEX5165154.1"
                     /translation="IGELDAQVADSAFQIAPPATELLLETNIDDMNPEYYELLIERLF
                     EAGALDVFLTPIVMKRGRPATKLSAIVAATDRARVEQTLIENSSTFGVRALAIERTKA
                     GRDWETVATRWGDVRLKLKIWRGRVLEAVPEYADCVAVARAADAPLRLVYGEAQRIGD
                     VFVGQRRSGIDGAGKGNDE"
BASE COUNT          428 a          835 c          743 g          398 t
ORIGIN      
        1 gccgcaactt cggctggagc cagatggagg ggcagcactg cttcgccgcg gactgcgacg
       61 cgagcctcta cacgctcccg atcgcggaat acagccacga cgacggctgt tccgtcaccg
      121 gcggctacgt ctaccggggc agcgactacc ctgcgttggc cggtcgctac ttcttcggcg
      181 actactgtag cggcaagatc tggtcgctga caccggatgg cgacggctgg acaatgacgc
      241 cggaactgga caccgactcg tggattacct ccttcggcga ggacgaagcc ggcgagctct
      301 acctggtcga cctggcaggc gtcctctatc gcgtcacgac ttccgaactg caatcgccca
      361 gcatcgacaa tccagccatg ctgcggacct ggcaacggac cgacaagccc gttgccgacc
      421 tgaccgtcga tcgcacctgg atgtgggggc cggagccatt cacgaacgcg attcccgaac
      481 cgtacgccga atcgccggat ggctcgcgaa tggtccagta cttcgacaag gcgcggatgg
      541 agatcaccca gcccgacgcc gaggatgacg gcatctggta cgtcacgaac ggcctgttgg
      601 tcgtcgagat gatgagcggc cagatccaga ctggcgacaa ccggttcgaa tcacagcagc
      661 cggccaccga aaacgtcgcc ggcgaccccg gcgtcgagtc aggaccaacc tacgcgaccc
      721 tcgcgccact ccgcggcgca ccacccgccg cagacagcgc gacgctgaca cagcggatcg
      781 caccggatgg caccgtctcc gacgacccga cgatggcgtc gtttggcatc acggccgccc
      841 aacgggtgca ggtgccgcac atcgaccacc agatcgcctc ggtcttctgg gagttcatga
      901 actcaaccgc gaccgtctac gaggacggcg aactggtcaa cgacctgctg ttcgtcaacc
      961 cctactacgc caccggctac ccgatcaccg aggcgtactg gacgacgatc ctgctggaca
     1021 gcaacgagac cgacgtcctg ctgcaatgct tcgaacgccg ctgcctgacc tacacgcccg
     1081 gcaatgaacc gggctggcag gttgaagccg gcaacgtcgg gcaacactac taccgctggc
     1141 ggtatgggag ctgagcgcat cgggacgtgg gacgcgggac gcgagccagc cgtccggcgt
     1201 tggtgtagac acgaacgcgc accctgtcat cccgacgaag gagggatctc gttctcgttg
     1261 acgcgggcat agaccgaacg aggacgagat ccttcgctga cgctcaggat gacagggcac
     1321 ggggtgcggt cctcgtggga gccgtccacc tgggtcgcat cccgcgtccc gcgtcccacg
     1381 tcccgcgtcc cgatgcgatc acttcccggc gaggcgctgg cggtgttcct cgacggaggc
     1441 gcggaccttc tcgtcgccgg tggcgatgat ctgggcggcc aagatggcag cgttgcgggc
     1501 gccgcccttg ccgatcgcga cggttgcgac cgggatgcct ggcggcatct ggacgatggc
     1561 cagcagcgcg tcgagcccgt tcaggtcgct ggtcgccagc gggacgccga tgaccggcag
     1621 cgtcgtccag gcagcgatcg tgcccggcag ggcggccgcc cagccggcgc cggcgatgat
     1681 gacctcgacg ccccgatcgc gggcggttga cgccatctcc tgaaccttgg cgggtgtccg
     1741 gtgggcggac atcacgaccg tcgtgctggg gatgcccaat tcgtccagca tcttctccgc
     1801 ctcggtcatg aacgggcggt cgctctcgct gcccatcacg atcaccaccc gcggcttgtc
     1861 gctactactc atcgtttccc tttccagctc catcgatacc tgaccgccgt tgcccaacga
     1921 agacgtcgcc gattcgttgg gcctcaccat agaccagccg taacggtgca tcggctgccc
     1981 gggcaactgc tacgcaatct gcgtactcag gcactgcttc cagcacccgc ccacgccaga
     2041 tctttaattt gagccggaca tcgccccagc gcgtcgcgac cgtctcccag tcgcgcccag
     2101 ccttggtccg ctcgatcgcc agcgcgcgaa cgccgaacgt cgatgagttc tcgatcagcg
     2161 tctgctcgac ccgcgcgcga tcggttgccg cgacgatcgc gctcagcttc gtcgccggcc
     2221 gcccacgctt catgacgatc ggtgtcagga agacatccag cgccccagcc tcgaacaacc
     2281 gctcgatcaa caactcgtag tactcgggat tcatgtcgtc gatgttggtt tcgaggagga
     2341 gttcggttgc cggaggtgcg atctggaacg cgctatcggc gacctgcgcg tcaagctccc
     2401 caat
//
LOCUS       DASUGJ010000319         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1716389, whole genome shotgun sequence.
ACCESSION   DASUGJ010000319 DASUGJ010000000
VERSION     DASUGJ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716389"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..1269)
                     /gene="glpX"
                     /locus_tag="VFV93_07160"
     CDS             complement(271..1269)
                     /gene="glpX"
                     /locus_tag="VFV93_07160"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX5165155.1"
                     /translation="MSGNQPTQPDRNLAMELVRATEVAALAAARWMGRGDKIAADQAA
                     VDGLRTALATIEMDGIVVIGEGEKDEAPMLYIGEEVGKGKEPRVDIAVDPVEGTRLLS
                     NGMPNAISVVALSERGTMHYPAKIAYMEKLATGPEAAGAIDIERPLKENLERVAKAKG
                     MGVRDLTVVVLDRPRHAEIVEEIRAAGARIKFITDGDVAGSLMAAMPDTGLDLLVGIG
                     GATEGTISACALKCMGGNLQSRIWPRNDEERQLIQEMNLDVTKVYGMDDLVSSNNVFF
                     AATGITDGELVAGVHYSGTGVTTESLVMRSKSGTIRRVSATHRLDKLGLFAATRFD"
     gene            complement(1266..>2936)
                     /locus_tag="VFV93_07165"
     CDS             complement(1266..>2936)
                     /locus_tag="VFV93_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX5165156.1"
                     /translation="RLAEEEGSCIPGQCVFHRRNQCFLFRARQEAEAAHIVIVNHSLL
                     ISDLLARHSVIPTYRQLVIDEAHHLESEATDRVGYSISRGQASDLIHRVVTESEPLGI
                     GGALGLTFRSVAGQGGEHSRTVAAELQTRLVEAHANAKGCSDGIDRFFNALGDFMERY
                     EHESTSYDRQLRLTSAARRDPGWSQLEIEWDDLMQPLGRLIEDLRFFDRAIDGFSDDE
                     IPTRPEMKTEFEVLQQDIDGLRERMTEFVSTPSAEMIYWLSRRLATDETSGHAAPLHV
                     GGILNERLFNRCESVTLTSATLTTDGSFDYIRDRLSLEDADELRVPSPFDYARSALLA
                     VTDDIPEPGQPGHQKKLQESIIEICSASGGRAMVLFTSHSALQSTYRAIKRTLEARNI
                     LVLGQRIDGSPRQLIDRLKSNPRTILLGTNSFWEGVDIVGDALSLLVITKLPFPVPSD
                     PVFAARSEQFEEPFAQYAVPQAILRFKQGFGRLIRSAEDRGACAVLDRRIISRRYGDA
                     FMNSLPSCEVTIGTTDRVAMSIHDFLDRRMELTTGRLPLEDERQEDVS"
BASE COUNT          504 a         1007 c          865 g          560 t
ORIGIN      
        1 ctgttcgctc acgacatcgc cgctctgctc ttgggactcc attactcacc gtactgttaa
       61 cagcgcgccg gcgcggcacg cacacggccc ggacgaaccc gggcaatcga acgctgggct
      121 ccgtctccgt caaatgagac acgtatggat acaggcgcgg aaggatcagt cacacggatg
      181 ggtcttcaat cgattgtgtt gctgatatac acccgaggct ggagggtggg atgcccaccg
      241 ctcgcggcca ctatatcagc atttccgcaa ctagtcaaac cgcgtcgccg cgaacagccc
      301 gagcttgtcg agccggtgcg tcgcgctgac gcggcggatc gtacccgact tcgagcgcat
      361 caccagcgac tcggtcgtca caccggttcc tgagtagtga actccggcca ccagctcgcc
      421 gtcggtgatg ccagtcgccg caaagaacac gttattggag ctgaccaggt cgtccatccc
      481 gtagaccttg gtgacgtcca ggttcatctc ctgaatcagc tggcgttcct cgtcattgcg
      541 cggccagatc cggctctgca ggttgccgcc catacacttc agcgcgcagg ccgagatcgt
      601 cccctcggtg gcgccgccga tgccgaccag cagatccagg ccggtgtctg gcatggccgc
      661 catcagcgag ccggccacat cgccatcggt gatgaacttg atccgcgctc cggccgcccg
      721 gatctcctcg acgatctctg cgtgacgtgg ccggtcaagg acgacgaccg tcagatcgcg
      781 gacgcccatc cccttcgcct tggcgacgcg ctccagattc tccttcagtg ggcgctcgat
      841 gtcgatcgcg ccggccgctt ccggcccggt cgccagcttc tccatgtagg caatcttggc
      901 tgggtagtgc atcgtcccgc gctccgagag cgcgacgacc gagatcgcgt ttggcatgcc
      961 attcgaaagc agccgggtgc cttcaactgg atcgacagcg atgtcgacgc gcggctcttt
     1021 gcccttgccg acttcctcgc cgatgtagag catcggcgcc tcgtcctttt cgccctcgcc
     1081 gatgacgacg atgccgtcca tctcgatcgt cgccagcgcc gtccgcaggc catcgacggc
     1141 tgcctggtcg gcggcgatct tgtcaccacg ccccatccag cgcgcggctg ccagagctgc
     1201 gacctcagtc gcccgtacca gttccatcgc caggttgcga tccggctgcg tcggttgatt
     1261 tccgctcatg acacgtcctc ctgtcgctcg tcctccagtg gaaggcgtcc tgtcgtcagt
     1321 tccatgcgcc ggtcaagaaa gtcgtggata ctcatcgcga cccgatcggt cgtcccgatc
     1381 gtcacttcac agctcggcaa cgagttcatg aacgcatcac catagcgccg gctgatgatg
     1441 cgccggtcca gcaccgcgca agcgccgcga tcctcagcgc tgcggatcaa ccggccgaaa
     1501 ccctgcttga agcgcaggat cgcctgcggg accgcgtact gcgcgaacgg ctcctcgaac
     1561 tgctcgctgc gggccgcgaa gactggatcc gacggaaccg gaaacggcag cttcgtgatc
     1621 accagcaggc tcaacgcgtc gccgacgatg tcgacgccct cccagaacga gttggtgccc
     1681 agcagaatcg tgcgcgggtt cgatttgagc cggtcgatca actgccgtgg cgaaccgtcg
     1741 atcctttgcc ccagaacgag gatgttacgc gcttccagcg tccgtttaat ggcccggtac
     1801 gtcgattgta gcgcgctgtg cgaggtgaac agcaccatcg cccgtccgcc ggaggcgcta
     1861 cagatctcga tgatcgactc ctgcagtttc ttctgatgcc ccggctggcc cggctccggg
     1921 atgtcgtcgg tcaccgccag cagcgccgac cttgcatagt cgaagggcga cggcacccgt
     1981 aattcgtcgg catcctccag tgagagccgg tcacggatgt agtcgaatga tccgtccgtc
     2041 gtcagcgtcg ccgatgtgag cgtgacactc tcgcagcggt tgaacagccg ctcgttcagg
     2101 atcccgccga cgtgcagcgg cgcggcgtgg ccgctcgtct cgtcggtcgc cagtcgacgc
     2161 gagagccagt agatcatctc ggcacttggt gtcgagacga actcggtcat ccgctcccgc
     2221 agcccatcga tgtcctgttg cagcacctcg aactcggtct tcatctccgg ccgcgtcgga
     2281 atctcgtcgt cgctgaagcc gtcgatcgcg cggtcaaaga aacgcagatc ttcgatcaac
     2341 cgcccgagcg gctgcatcag atcgtcccac tcgatctcca gctgcgacca gcctggatcg
     2401 cgtcgtgccg ccgaggtcag tcgcagctgc cggtcatagc tggtgctctc gtgctcgtaa
     2461 cgctccatga aatcaccgag cgcgttgaag aagcggtcga tcccgtcgct gcaacccttc
     2521 gcgttcgcgt gcgcctcgac cagccgcgtt tgcaactccg ccgcgacggt ccggctgtgc
     2581 tcaccaccct gaccagccac cgaccggaat gtcagcccga gtgccccgcc gatccccagc
     2641 ggttccgact ctgttacgac acgatggatc agatcgctgg cctgcccacg cgagatgctg
     2701 tagccgaccc gatcggtcgc ctcagactcc aggtgatggg cttcgtcgat taccaattgc
     2761 cggtaggtcg ggatgaccga gtgccttgcg agcaaatccg agatcagcag cgagtggttg
     2821 acgataacga tgtgggctgc ttcagcctcc tgccgcgcac ggaacaggaa acactggttg
     2881 cgacggtgga agacgcactg gcccgggatg catgagccct cttcctcggc cagcct
//
LOCUS       DASUGJ010000320         3670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1854658, whole genome shotgun sequence.
ACCESSION   DASUGJ010000320 DASUGJ010000000
VERSION     DASUGJ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854658"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..220
                     /locus_tag="VFV93_07170"
     tRNA            146..220
                     /locus_tag="VFV93_07170"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:178..180,aa:Ile,seq:cat)
     gene            284..631
                     /gene="rsfS"
                     /locus_tag="VFV93_07175"
     CDS             284..631
                     /gene="rsfS"
                     /locus_tag="VFV93_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX5165157.1"
                     /translation="MTDPIEIAREVAEIASDTLATNITVLDISQISSFADVFVVCSAD
                     NVRQLNALREAIVTKLNEQGVRTRRAEGTAETGWVLLDYGDVIVHLFTDEQRMFYRLE
                     DVWTEAQKVLVIQ"
     gene            674..838
                     /locus_tag="VFV93_07180"
     CDS             674..838
                     /locus_tag="VFV93_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165158.1"
                     /translation="MARTRSVVIISLAATLAVVTAILVAARMSSSSWGALMRLFRDDN
                     DHPHWELLAQ"
     gene            complement(843..1298)
                     /locus_tag="VFV93_07185"
     CDS             complement(843..1298)
                     /locus_tag="VFV93_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5165159.1"
                     /translation="MPIYEYRCRACGRMASLFFRSVATVDPDPACPHCGSHELTRRMS
                     RVWSWRSRDDIPQPTFEDEGVPFYGPDPFSEPYGDFDDFGGDGDSEENIAAVAREARA
                     MAEMMGEPLDAEFDAALRHVEQGADPDDVFGELDAVESTRESQPENGQT"
     gene            complement(1285..2376)
                     /locus_tag="VFV93_07190"
     CDS             complement(1285..2376)
                     /locus_tag="VFV93_07190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX5165160.1"
                     /translation="MSHALIAAIVGLLAAASAAVTVPPIRDIARRLELLDQPEARRVH
                     TIPIPRVGGLAIFFGFLVAVGVSFVLPVERFGDEIERILLLMIGATMIVGVMLFDDVV
                     GIPPLPKLAVQLAAAGLVVLPRLRGSSHGIVIEQFNAPYVGTVELPLLIAIGFTLFWI
                     VGMMNAINWSDGIDGLAASITLVATVILFLHTYFRPAGDPQFTISLLAIALAGAIVGF
                     LIYNWHPASIIMGDTGAMFIGFALATISIIGGAKIATALLALGVPILDTAWVILYRVM
                     HGRSPLGADRGHLHHRLLDAGWSQRQIVAGVAGITALFGALGLALPTREAKLGAMIVM
                     GLVLLSLIAWLARRGQSDPGRVRVPHADL"
     gene            complement(2389..3648)
                     /gene="glyA"
                     /locus_tag="VFV93_07195"
     CDS             complement(2389..3648)
                     /gene="glyA"
                     /locus_tag="VFV93_07195"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5165161.1"
                     /translation="MEALAQGDRQIYDIVRAEERRQLDTIELIASENFTSAAVMEAVG
                     TVLTNKYAEGLPGKRYYGGCEFVDQVETLAIERAKALFGANNVNVQPHSGASANVAVF
                     TALLEPGDKILGMNLSQGGHLTHGSPVNFSGKFYEAHFYGVDPDTETIDYDAVLSTAK
                     EVRPKLIICGASAYSRTIDFARFRDIADEVDALLMADIAHIAGIVAVGLHPNSIGNAH
                     ITTTTTHKTLRGPRGGMIMTGDEHAAAINKSVFPGMQGGPLMHVIAGKAVAFGEALQP
                     SFKTYIERVLANAAALAESVQEHGLRVLSGGTDNHLMLVDLTEAGISGRKAERALERA
                     GITANKNAIPNDPRPPVQTSGIRLGSPAMTTRGFGVDEMRQVGAWIGEIVRSPDDEEL
                     IARIRGQVTEMCAGFPLPGVERGSAVR"
BASE COUNT          652 a         1177 c         1193 g          648 t
ORIGIN      
        1 gagagacgcc gttgcggtgg ccgaaatccg gcctgacgta tacttaggcg gtcccgcagg
       61 ggccggacgt ggatggctga gcacgaaagt tgcgacagtc gcggggctct cgccgttggc
      121 ccagcgcgcg acgaccggcg gacgcgggcc tgtagctcag tggcagagca aggggctcat
      181 aacctcatgg tcgcaggttc gaaccctgcc aggcccacca tcgtcctatt gcaaccgcga
      241 acgttcgcag gtatgatgac tgtacgtaca gttcaggagt atgatgaccg acccaatcga
      301 gatcgcccgt gaagttgctg aaattgcgtc cgacacactc gcgaccaata tcaccgttct
      361 ggacatcagc caaatctcat cgttcgcaga tgtcttcgtc gtctgctcgg ccgacaatgt
      421 ccggcaactc aatgcgctac gcgaggcgat cgtcacgaag ttgaatgagc agggtgtacg
      481 gactcgccgc gccgaaggca ccgccgaaac cggttgggtc ctgctcgact acggcgacgt
      541 gatcgtccac ctattcacgg atgaacagcg catgttctat cggctggaag acgtctggac
      601 cgaagcgcag aaggtgctcg tcatccagta gcgcacgaca agggtcgatg cggacggagg
      661 aggcgtgagt ctcgtggcgc gtactcgttc ggtggttatc atcagcctgg ccgcgacgct
      721 cgcggtcgtc acggcgatcc tcgtcgccgc ccgcatgagc agttccagct ggggcgcgct
      781 catgcgcctg ttccgtgacg acaacgatca cccgcactgg gaattgctgg cacagtagcg
      841 cgttacgtct ggccgttctc cggttggctc tcgcgtgtgc tttcgaccgc atccagttcc
      901 ccgaagacat cgtcggggtc ggcgccttgc tcgacgtggc gcagggcggc gtcaaactcg
      961 gcgtcgagcg gctcgcccat catctctgcc atcgctcgcg cctcgcgcgc cacggccgcg
     1021 atgttctcct cgctgtcgcc gtcaccgcca aaatcgtcga aatcgccgta tggctcgctg
     1081 aacgggtctg ggccgtagaa cgggacgccc tcgtcttcaa acgtcggttg cgggatgtcg
     1141 tcccttgagc gccacgacca gacgcgcgac attctgcgtg tcagttcatg tgaaccgcag
     1201 tgcgggcagg cggggtcagg gtcgacggtc gccaccgagc ggaagaagag actcgccatc
     1261 cggccacagg cacgacagcg gtactcatag atcggcatgt gggaccctca ctcgaccagg
     1321 atcggattgt ccacgacgcg ccagccaggc gatcaacgag agcagcacca accccatcac
     1381 gatcatcgcg ccgagctttg cctcgcgcgt cggcagcgcc agcccgagcg cgccgaagag
     1441 ggcggtgatg ccggcgacgc cggccacgat ctgacgctgg ctccagccgg cgtccagcag
     1501 ccggtggtgc aggtggccac ggtcggcgcc cagcggcgac cgaccatgca tgactcggta
     1561 gagaatgacc caggccgtgt caaggatcgg gacgcccaat gccagcagtg ccgtcgcgat
     1621 cttggctccg ccgatgatgc tgatcgtcgc cagcgcgaag ccgatgaaca tcgcgccggt
     1681 atcgcccatg atgatgctgg ctgggtgcca gttgtagatc aggaagccga cgatcgcgcc
     1741 ggccagcgcg atcgccagca gcgagatcgt gaactgcggg tcgccggccg gccggaaata
     1801 ggtgtgtaga aacaggatga cggttgccac cagcgtgatg ctggccgcca ggccgtcgat
     1861 gccgtcgctc cagttgatcg cgttcatcat cccgacgatc cagaacagcg tgaagccgat
     1921 cgcgatcagc agcggcaatt cgaccgttcc gacgtagggt gcgttgaact gctcgatgac
     1981 gatcccgtgg ctgctgccgc gcaggcgtgg caggacgacc agcccggccg ctgccaactg
     2041 gacggccagc ttcggtagcg gcggtatgcc gacgacgtcg tcgaacagca tcacgccgac
     2101 gatcatcgtt gcgccgatca tcaagagcag gatgcgctcg atctcgtcac cgaaccgctc
     2161 gaccggcagg acgaacgata cgccgacggc caccaggaag ccgaagaaga tggctagccc
     2221 gccgacacgc ggaatcggaa tcgtgtggac gcgccgcgcc tcgggttggt cgagcagttc
     2281 gagccgtcgc gcgatgtcgc ggatgggcgg cactgtcaca gcagcggacg cagcagccag
     2341 gagcccgacg atggcggcga tcagcgcgtg tgacatcgct gcgcccggct agcggacagc
     2401 ggagccacgc tcgacgccgg gcagcgggaa gccagcacac atctccgtca cctggccacg
     2461 gattcttgca atcagctcct cgtcgtcggg cgagcggacg atctcaccga tccaggcccc
     2521 cacctggcgc atctcatcaa ccccgaaccc gcgagtcgtc atggccggcg agccaagccg
     2581 gattccgctg gtctgcaccg gaggacgcgg gtcgttcggg atcgcgttct tgttggcggt
     2641 gatgccggcc cgttcgagcg cccgctcggc cttgcggccg ctgattccgg cctcggtcag
     2701 gtcaaccagc atcaggtggt tgtcggtccc gcccgaaagc acccgtagtc catgctcctg
     2761 gaccgattca gccagcgcgg cggcgttggc cagcacgcgc tcgatgtagg tcttgaacga
     2821 gggctgcaac gcttcgccga acgcgacggc tttgccagcg atgacgtgca tcagcggccc
     2881 gccctgcatg ccggggaaga cggacttgtt gatcgcggcg gcgtgctcgt cgccggtcat
     2941 gatcatcccg ccacgcggcc cgcgcagcgt cttgtgggtt gtcgttgtcg tgatgtgcgc
     3001 gttgccgatc gagttcggat gcagtccaac ggcgacgata ccagcgatat gggcgatgtc
     3061 tgccatcaag agcgcgtcga cctcatcggc gatgtcgcgg aagcgggcga agtcgatcgt
     3121 tcgcgaatag gcgctggcac cgcagatgat cagctttggc cgcacctctt tggccgttga
     3181 caggacggcg tcgtagtcga tcgtttcagt gtctggatcg acgccgtaga aatgtgcctc
     3241 gtagaacttg ccggagaagt tgaccggcga gccgtgggtg agatgaccgc cctgagagag
     3301 gttcatcccc aggatcttgt cgccaggctc cagcagcgcg gtgaagaccg cgacgtttgc
     3361 ggacgcgccg gagtgcggct gaacgttgac gttgttcgcg ccgaagagtg ccttcgcccg
     3421 ctcgatcgcc agcgtctcga cctggtcgac gaactcacag ccgccatagt agcgcttgcc
     3481 cggcagccct tcggcgtact tgttcgtgag gaccgtcccg accgcctcca tgacggcggc
     3541 gctcgtgaag ttctccgacg caatcagttc gatggtgtcg agttgccggc gctcttcagc
     3601 gcggacgatg tcgtatatct gtcggtcccc ttgtgccagt gcttccacgc tacccccgct
     3661 gtctcaggct
//
LOCUS       DASUGJ010000321         6420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_48889, whole genome shotgun sequence.
ACCESSION   DASUGJ010000321 DASUGJ010000000
VERSION     DASUGJ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48889"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VFV93_07200"
     CDS             complement(<1..690)
                     /locus_tag="VFV93_07200"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5165162.1"
                     /translation="MSSSPDSSVDLMREALAYVDAWLDYRVWKLRTPGAQVAVWFDGG
                     VQFSKAYGLSNLDTGEALTTSHLFRIASHSKTFTATAVMQLVEAGKLRLDDTAGTWIP
                     ALAEGDSPLAEVTVRELVSHSAGVIRDGVDATYWAHSRPFPNEDELLALALREGVVRQ
                     PEATFKYTNIGFSLLGMIVGKAAGSSYNEYTKTAIVEKLGLRNTGPELDEARLSEYAG
                     GHSGLSSWTARQ"
     gene            885..1883
                     /locus_tag="VFV93_07205"
     CDS             885..1883
                     /locus_tag="VFV93_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5165163.1"
                     /translation="MAKNISMTSIAFEHVVKRFPGSSAPAVDDVTFTVPAGAICMLLG
                     TSGSGKTTLLRMVNRLSDPTSGRVAIDDVPTVAQDPIALRRRIGYVIQQVGLLPHLTV
                     AENVGIVPAITGDRSTTIQRRVDELLELVGLPPGEYRKRYPAQLSGGQAQRVGLARSL
                     AANPSVLLMDEPFGALDAITRQRMQDELRQIQREMRKTILFVTHDVDEAFRLGDCIAV
                     LSAGRLEQFGTPVDLLTAPATPFVNQLVGADNILRQFEYLPVTAALEPAIQIDDATER
                     IPAGETLLGAMLRLLESGHPALAVEADGQIQGTISLTSISRAVRGQAVLAPELGRR"
     gene            1880..2542
                     /locus_tag="VFV93_07210"
     CDS             1880..2542
                     /locus_tag="VFV93_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5165164.1"
                     /translation="MNYILDSRNWELADPTSIGRLLIDHLVLTGMSMLIALAIAFPLA
                     IVSARYPRVYLPVITVAGILYTLPSLAFMAALIPFTGLTTTTVVIPLVLYAQVVLIRN
                     IVAAIHAVDPGLIDIGRAMGMSARQLQRYVVLPLALPVIVAGLRIATVTTIGIATIAP
                     LFGVRNLGYLIFQGFNTSYHEQVLAGVIMVSALAIAADLLLLLIQRSLDRGQRAAVAG
                     RA"
     gene            2558..3220
                     /locus_tag="VFV93_07215"
     CDS             2558..3220
                     /locus_tag="VFV93_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5165165.1"
                     /translation="MGDVLDFLRDPQNHFVRQTTTYLRLCAVPIAFSILIGLVLGVLV
                     ARRSFAAFVVVNLSGLVRAIPTLAFLAVVLPYLGIGFTPAVVALTALGVPPVLLNTIA
                     GLRGIDPAVIDSARGMGMTPRQVLTRVEVPLVLPVVAAGIRTAAVQIVATAPLAALIG
                     AGGYGDYILAGVNLLQRTPLLVGAGSVALLALAVEVALAGVERAVTPAGLRVRGPTSS
                     GA"
     gene            3272..4183
                     /locus_tag="VFV93_07220"
     CDS             3272..4183
                     /locus_tag="VFV93_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5165166.1"
                     /translation="MFSRNAWRLPVVVLVSIALLGLAACGDDDDSGSDKASLTIGSKL
                     DVDGQLNAQMYALLLEAKGYDVSTKLGLGQTPVLDQAIKSGDIDIYPEFTGTALTTLN
                     LEPTQDAQQAYEQVKAAYEEQFELTMLEPAYELNDSYAICTSQDVAAEHGLQSLDDLA
                     PVAGDLKIAAQQDGIDAAINPVQTAYGFEFGEVVQLSAQLSFDAVKGGDVDLNVCYTT
                     DPAIVVNDFVVLEDTKNVFPIYNPAPLVRDDVLKESPDIAETLNALQPHLTTEVILDL
                     IKQVSVDHESVGDVARAFLEQMGLIEK"
     gene            4365..>6420
                     /locus_tag="VFV93_07225"
     CDS             4365..>6420
                     /locus_tag="VFV93_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5165167.1"
                     /translation="MEGSASEAETGVDAGRDRHVRARPVGRARERGNLRGVLRQMLDG
                     HGRLVLVSGEAGIGKTTLVADLADDAEAQGCLAMWGHAYDLSVTPPYGPWLELLRRYP
                     GQDDGFPPLPPFVDDARMLATVTSQQALFSAVTAFFQDVARRMPLVLVLDDLHWADQA
                     SLDFLRFLAREIGSHRILIVATYRSDEIDQGHPLYALLPPLVRESGVERLDVRGLDTE
                     DHLALVASRYAMRGEDTDRLAQHLETHAEGNPLFALELLRSLEEEHILHFTDGGWVLD
                     DIAQTRVPPLLRQVIDRRLSRLDNDARALLQVAAIIGQQASLDLWQQVTEATDEALIL
                     AIEQSAAAALLEEVESGASYRFQHALFREALYAEVAAPRRRTWHRRVAEALAARPHPD
                     PDAVAFHFQQAGDARATDWLLQAARRAKQSYARRTAASRLEAAARMLQQQRGADPREY
                     YLLLYEIGSILRYTSSPDVTSYLAEASAAARQAGDAAVAAIADAELGYTHLLQGDVQR
                     GIGEIERALAECRRLLRADPTVFDHAAVEFSLNAEAITLADRLATRTAILLLAGAFVG
                     SLDQTLPAVEQFGTTVDEVFQRDATVPYALTANGIPYQLADLTWAIAMADALLGRVAD
                     AQRRFSYAEQIFSNDGEHYLVMGVNFWSLTRVELPYLADNPAATHARADAARAALSRS
                     LNV"
BASE COUNT         1148 a         2102 c         2010 g         1160 t
ORIGIN      
        1 ctggcgggcg gtccagctgc tcagcccgct gtgaccgccc gcgtactcgg acaggcgcgc
       61 ctcgtccagt tccggccccg tgttgcgcag gccgagcttc tcgacgatgg ccgtcttggt
      121 gtactcgttg tagctgctac ctgccgcctt gccgacgatc atgccgagca atgagaagcc
      181 gatgttggtg tacttgaacg tcgcctccgg ctggcggacg acgccctcgc gaagcgcgag
      241 agccagcaac tcgtcttcgt tcgggaatgg ccggctgtgc gcccaatagg tggcatcgac
      301 accgtcgcga atcacaccgg ccgagtgtga gacgagttcg cgcacggtca cctccgccag
      361 cggcgaatcg ccctccgcca gcgccggaat ccacgtcccg gccgtgtcgt ccagccgcag
      421 cttgccggcc tcgaccagct gcatgacggc cgtcgccgtg aacgtcttcg agtgcgaggc
      481 aatccggaac agatgcgacg tcgtcaacgc ctcacccgtg tcgaggtttg agagaccgta
      541 ggctttgctg aactggacgc cgccgtcgaa ccagaccgcc acctgcgcgc ctggtgtgcg
      601 aagcttccag acgcggtagt cgagccaggc atcgacatag gcaagcgcct cacgcatgag
      661 atcgaccgaa gaatctggcg aactggacat gcactcactc catcacctgc acgtagagaa
      721 gacacacgaa cgcgttcgcc cgatgggacg aacgccaacc ggtgcaggct atcggagttg
      781 ccgcagcggt gcaatggggg agatatcgtc gagtatcgtg tcgtggccct tctcttgcag
      841 cacgtcacat tgcttacaga gaagcgatgc cggggcagtg atgggtggcg aagaacatta
      901 gcatgaccag catcgcgttt gaacacgtcg tgaagcgctt ccccggctcc agcgcaccag
      961 cggtcgatga tgtcacgttc accgtaccag ccggcgcgat ctgcatgctg cttggcacgt
     1021 caggttcggg caagacgacg ttgctccgga tggtcaaccg gctgagcgac ccgacctctg
     1081 gccgggtagc aatcgacgac gtgccgaccg tggcgcaaga cccgatcgcg cttcggcgac
     1141 ggatcggcta tgtcatccag caggtcgggc tgttgccaca tctgacggtc gcagagaacg
     1201 tcgggatcgt cccggcgatc accggcgacc gctccacgac gatccagcgc cgcgtcgacg
     1261 agttgctgga actggtcggc ctgccgcccg gcgagtatcg caagcgatac ccggcacaac
     1321 tgagcggcgg tcaggcacag cgcgtggggc tcgcgcgttc actggctgcc aacccgtccg
     1381 tgctcctgat ggacgaaccg ttcggcgcgc tggacgcgat tacgcgtcag cgcatgcagg
     1441 acgagttgcg gcagatccaa cgcgagatgc gcaagaccat cctcttcgtg acgcacgacg
     1501 tcgacgaagc cttcaggctc ggtgactgca tcgcggttct gtcggctgga cggttggaac
     1561 agtttggaac cccggtcgac ctgctgaccg caccagccac accgttcgtg aatcagttgg
     1621 tcggtgctga caatatcctg cgccagttcg agtacctgcc ggtgacggcg gcacttgagc
     1681 cggccatcca gatcgacgat gcgaccgagc gcatccccgc cggcgagacg ctcctggggg
     1741 cgatgttgcg gctcctggag tcaggccacc ctgcgctggc ggtcgaagcg gacggacaga
     1801 tccagggaac aatcagcctg accagcatct cgcgagcggt acgcggccag gcggttctcg
     1861 ctccggaatt gggacgccga tgaactacat cctcgactcc cgaaactggg agctggccga
     1921 cccaaccagc atcggccggt tgctgatcga ccacctcgtc ctgaccggaa tgagcatgct
     1981 gatcgcactg gcgatcgcct ttccactggc catcgtctcg gcccgttacc cgcgcgtgta
     2041 cctgccggtc atcaccgtgg caggcattct ctacaccctg ccgagcctcg ccttcatggc
     2101 ggcgctgatc ccgttcaccg gtctcacgac gacgactgtc gttatcccgc tggtgctcta
     2161 cgctcaggtg gtgctgatcc ggaacatcgt cgccgccatt cacgccgttg accccggcct
     2221 gatcgacatc ggacgcgcga tggggatgag cgcgcggcag ttgcagcgct acgtggtgtt
     2281 gccgctcgcc ctgccggtca tcgtcgccgg actgcgcatc gcgacggtga cgacgatcgg
     2341 catcgcgacc atcgcgccgc tgttcggcgt gcgcaacctg ggctacctga tcttccaggg
     2401 cttcaacacc agctaccacg agcaggtgct ggccggcgtc atcatggtgt cggcgttggc
     2461 gatcgccgcc gatctgctgc tgctcctgat ccagcgatcg ctggatcgtg gccagcgcgc
     2521 cgctgttgca ggtcgagcgt aaggggaaga ggccagattg ggcgacgtcc tggattttct
     2581 ccgcgacccg caaaatcact tcgtcagaca aacgacgacc tatctgcgcc tgtgtgccgt
     2641 gccgatcgcg ttctccattc tgatcggcct ggtgcttggg gtgctggtcg cccgccgctc
     2701 gttcgccgcg ttcgtggtgg tcaacctcag tgggcttgtg cgcgccattc cgacgctcgc
     2761 cttcctggcg gtcgtcctgc cctacctggg catcggcttc acacccgcgg tcgttgccct
     2821 caccgcgctc ggcgtcccac cggtgttgct gaacacgatc gccggtctgc gtggcatcga
     2881 tccagccgtc atcgacagtg cacgtgggat ggggatgaca ccacgccagg tgctcacacg
     2941 cgtcgaggtc ccgctggtgc tcccggtcgt cgcagccggc atccgaaccg ccgccgtgca
     3001 gatcgtcgcc accgcgccgc tggcagcctt gatcggcgcc ggcggctacg gagactacat
     3061 tctggcaggc gtgaatctcc tgcaacgcac tccgctactg gtaggagccg gcagtgtcgc
     3121 gctgctggcg ctcgccgtcg aagtcgcgct cgccggtgta gagcgggccg tcacgcccgc
     3181 tggcctgcgg gtgcgtgggc ctacaagttc tggagcatag ttggttaccc tggactcaag
     3241 gtgcgtgttg ctgtgacatg gaaggaactc aatgttcagt cgtaatgcgt ggagactgcc
     3301 agtcgtcgtt ctcgtcagca tcgcgctcct cgggctcgcg gcctgtggag acgatgatga
     3361 ctcaggctcc gacaaagcca gcctcacgat cgggagcaag ctcgacgtgg acgggcaact
     3421 caacgcccag atgtatgcgc tgcttctcga agcgaagggg tacgacgtct cgacaaagct
     3481 ggggctgggg cagacaccag tcctcgatca ggcgatcaag agtggcgaca tcgacatcta
     3541 tccggagttc accgggacag ccctcaccac cctgaacctc gagccgaccc aggatgcaca
     3601 gcaggcctac gagcaggtga aggccgccta cgaggagcag tttgaactca ccatgcttga
     3661 accagcctac gagttgaacg atagctacgc aatctgcacg tcgcaggatg tggccgcaga
     3721 gcacggcctt cagtcactcg atgatctcgc tccggttgcc ggcgatctga aaatcgcagc
     3781 ccagcaggat ggcatcgacg cggccatcaa ccccgtccag acagcgtacg ggttcgagtt
     3841 tggtgaagtc gtgcagctca gcgcgcaatt gtcgttcgat gccgtcaagg gtggcgacgt
     3901 cgacctcaat gtctgctaca cgaccgatcc cgcaatcgtc gtgaacgact tcgtcgtgct
     3961 tgaggacacg aagaacgtct tcccaatcta taacccggcg ccactggtcc gcgatgacgt
     4021 gctcaaggag tcaccggaca tcgccgaaac gctcaacgcc ctccagccgc acctgacgac
     4081 cgaggtcatt ctcgacctca tcaagcaggt aagcgtcgat cacgagtcgg tcggagatgt
     4141 ggcccgcgcg ttcctggagc agatgggact gatcgagaag taacctgcca gcgcgataga
     4201 ccatgcggtc cgccgcgcga tgcataacga cgtattctcg atgaccgagc gtatggcagc
     4261 aaggctggtg aaacgctgcg acaatcttta aagaacggat gacttgcgca agcggagcat
     4321 cgcgcatacg atccgctcac agacattgag cggaggagtg agttgtggaa ggcagcgcca
     4381 gcgaggcaga gaccggtgtc gatgctggcc gcgacagaca tgtacgcgcc cggcccgtcg
     4441 ggcgtgcgcg ggagaggggc aatctccgcg gcgtccttcg acaaatgctc gacggccacg
     4501 gccggctcgt cttggtcagt ggcgaagccg ggatcggcaa gacgacactc gtcgcggacc
     4561 tggccgatga tgcggaggca cagggttgtc tcgccatgtg ggggcatgcc tacgacttgt
     4621 cggtcacccc gccatacggc ccctggctgg agctgctgcg gcgctatccc ggccaggatg
     4681 atggtttccc accactgcct ccgttcgtcg acgacgcccg catgctggca accgtcacga
     4741 gccagcaagc gctcttctcg gccgttaccg catttttcca ggatgtcgct cgccggatgc
     4801 cgttagtcct cgtcctcgac gatctccatt gggccgacca ggcgtcgctc gactttctcc
     4861 gctttctggc tcgcgagatc ggctcccacc ggatcctcat cgttgcaacc taccgatccg
     4921 atgagatcga ccagggacat ccgctgtacg cactgcttcc gcctctggta cgtgagtccg
     4981 gcgtcgagcg gctcgatgtg cgcggactcg acaccgagga ccatctggct ctggtcgcca
     5041 gccgctacgc catgcgcggc gaggacaccg accggctcgc gcagcacctc gagacccacg
     5101 cggaggggaa cccgctcttc gcgcttgaac tcctgcgcag tctcgaggag gagcacatcc
     5161 tccatttcac tgacggcggg tgggtactcg atgacattgc acagactcgc gttccacccc
     5221 tgctccggca ggtgatcgat cggcgattga gccggttgga caacgacgca cgggcactcc
     5281 tgcaggtagc ggccatcatc ggccaacagg cgtcgctcga tctctggcaa caggtcacgg
     5341 aggcaaccga cgaggcattg attcttgcta ttgagcaaag tgcggcggca gcattgctgg
     5401 aggaagtaga gagcggcgca agctatcgct tccagcatgc gctcttccga gaagcgctgt
     5461 acgccgaggt cgccgcgccc cgtcgccgaa cctggcaccg tcgggtggca gaggcgcttg
     5521 ccgcccggcc acatccagat ccggatgccg tcgcctttca ctttcagcag gctggcgacg
     5581 cacgagcaac cgactggctc ctgcaggccg cgcgccgtgc caagcagagc tatgcgcgac
     5641 ggacggcggc cagtcgcctg gaagcggccg ccaggatgct tcaacagcag cgcggtgctg
     5701 atcccaggga gtattacctg ctcctctacg agatcggatc gatcctgcgt tacacgtcct
     5761 cgccggatgt cacgtcctac ctggcggaag cttctgcggc agcgcggcaa gcgggagacg
     5821 cggccgtcgc agcgatcgcg gatgcggaac tgggctacac ccacctgtta caaggcgacg
     5881 tccaacgcgg cattggtgag attgagcggg cgcttgcgga atgccggcga ctcctgcgtg
     5941 ccgacccgac cgtcttcgat cacgccgcgg ttgagttcag cctgaacgcc gaggcgatca
     6001 cgttagcgga cagactggct acccgaacgg cgatcctcct gctcgcgggc gccttcgtgg
     6061 gttccctgga ccagactctt cctgcggtag agcagtttgg cacaacggtt gacgaggtct
     6121 ttcagcgcga tgccaccgta ccatatgcgc tgacggcgaa cgggataccg taccaattgg
     6181 ccgatctgac ctgggccatc gcgatggctg acgcgctgct cggccgggtt gccgacgccc
     6241 agcgccgctt cagctatgcg gagcagatct tcagcaacga cggggagcac tacctggtca
     6301 tgggcgtcaa tttctggtca ctgaccagag tcgaactccc ctatcttgcc gacaatccgg
     6361 cggcaaccca cgcacgcgcc gacgcggcca gggccgcgct tagccggtcg ctcaacgtcg
//
LOCUS       DASUGJ010000322         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1855010, whole genome shotgun sequence.
ACCESSION   DASUGJ010000322 DASUGJ010000000
VERSION     DASUGJ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855010"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFV93_07230"
     CDS             complement(<1..612)
                     /locus_tag="VFV93_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5165168.1"
                     /translation="MIEIKRGALIEAIAELVRTPSINPSLVPGAEGERAIAEQIAERL
                     RRTHGIEVELQDSGDGRPNVIASAGSGSGRTLMMNGHIDTVGVAGMAEPYSGRVEGNR
                     LYGRGALDMKGAMGGALVLLEAVAGDPSFPGRLIVTFVTDEEHSSIGTQAVCREIERL
                     RPDAAIVVEPSGLNGTIAHKGFAWAEIVTHGFAAHGSDWKHGVD"
     gene            complement(804..1337)
                     /gene="mug"
                     /locus_tag="VFV93_07235"
     CDS             complement(804..1337)
                     /gene="mug"
                     /locus_tag="VFV93_07235"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEX5165169.1"
                     /translation="MSGDGTAVRDLLRPGLSLVICGYNPSLRSAETGHNYAHPGNRFW
                     RVLHAAGITDRLYAPSEGRVLLEQGIGFTNIVQRPTRRADELSREEIRAGAAALREKL
                     ERYRPRAVAYTGIGVYKWLRGTTNVTWGIQPDSAVPGVTDIVVPSPSGLNRMTFDELV
                     EHYRVLADFLGRPPASK"
     gene            complement(1337..2209)
                     /locus_tag="VFV93_07240"
     CDS             complement(1337..2209)
                     /locus_tag="VFV93_07240"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5165170.1"
                     /translation="MGARLTAIRQMFLLEAVIAAPLMAGGFLVAGSRGVVGGLLVALL
                     IETVAWFCSRPILVRICSATPVADGEYPQLVAAVCRIADLAGITTPQVAVSTLPIPNA
                     MAAVTPGGGLVCVTEGLLGMLSPAELEAVLAHEIAHLTEPGRAGATIAALFASIPGAI
                     TAAAGSDLYFELPYRRAQMRVWGGGRMHPLRDLIARLTAPIAAILVRISTSPASEYAA
                     DERAARIIDDPAALALALRKLQSLAGRIVWPVNPAIAHLLVIHPFGCPKLGQLFNTHP
                     ATPDRLATLIDSTR"
     gene            complement(2209..>3402)
                     /locus_tag="VFV93_07245"
     CDS             complement(2209..>3402)
                     /locus_tag="VFV93_07245"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEX5165171.1"
                     /translation="KIGATTLLVAQSGGPTVVINASLAGVIEAARGSTEIGRILGARR
                     GVEGILTDDFVDLSELDDDRLERLRRTPSAALGTSRHRQSDAEVEQTLDCLQERNITI
                     FTPIGGNDTAETASRLASRAQARGQDLRIVAIPKTIDNDLPEMDHCPGYGSIARFIAL
                     AVRDATLDTLAMQQLYPVKIIEVMGRNAGWLAAAGSLAFDVQPELPRPVLCLPERPFE
                     SIEQLTALVTERIARDGFVVLVVPETMKWAGGQSAAGATPDWIDPFGHKYFSGVGNAL
                     ARELSARLGVRARYDKPGTIARMAMHAASELDLIEAAETGREAVRRALAGETGVMVTI
                     NRRADTPYYHVEYGTTPLERIASVERLLDDDMIDATGHDVTSRFSDWARPLVGAPLPV
                     YEVLS"
BASE COUNT          560 a         1186 c         1113 g          543 t
ORIGIN      
        1 gtcgacaccg tgcttccagt cgctgccgtg cgccgcgaag ccgtgcgtca cgatctcggc
       61 ccaggcgaat cccttgtggg cgatcgtccc gttcagtccc gatggctcga cgacgatcgc
      121 cgcgtcgggc cggagacgct cgatctcccg gcagacggcc tgcgtgccga tgctggagtg
      181 ctcctcgtcg gtcacgaacg tcacgatcag ccggccggga aagctcggat cgccggcaac
      241 cgcctccaac agcaccagcg cgccgcccat cgcgcccttc atgtcgagcg ccccgcgacc
      301 gtagagccgg ttgccctcga cccgtcccga gtacggctcg gccatcccgg ccacgccgac
      361 cgtgtcgatg tggccgttca tcatcaaggt tcggcccgac ccgctcccgg ccgaagcgat
      421 cacgttcggc cggccgtcgc cggagtcctg cagctcgacc tcgatcccat gcgtccgccg
      481 caaccgctcg gcgatctgct cggcaatcgc ccgctcgccc tccgcccccg gcaccagtga
      541 cggattgatc gacggcgtcc gcaccaattc cgcaatcgcc tcaatcaacg ccccgcgctt
      601 gatctctatc attcgttgtc ctcccctacc gtcggtcgat tgaacgacac caacgatgcc
      661 gccccaccgc aaccgcgtca acggccattc gacggggatc gatcatgttc caccatcaat
      721 ccactctcca aaccgagcag ggcgatattg cgatatcgcc cgtaccggat tggagagtgg
      781 attgagcgag tcacacgctt cgatcatttc gaagcaggag ggcgaccaag aaagtcagcc
      841 agcacgcggt agtgctcgac cagttcgtcg aaggtcatcc ggttgaggcc ggatggtgag
      901 gggacgacga tgtcggtgac gccgggaacg gccgagtcgg gctggatgcc ccaggtaacg
      961 ttcgtcgtgc cgcgcagcca cttgtagacg ccgatgccgg tgtaggcgac ggcccgcggt
     1021 cggtagcgtt ccagcttttc gcggagggcg gcggcgccgg cgcggatctc ctcgcgggac
     1081 agctcgtcgg cacgacgagt tggccgttgg acgatgttgg tgaaaccgat tccctgctcc
     1141 aacagcaccc tgccctcgct gggcgcgtag agcctgtcgg tgatgccggc cgcgtgcagg
     1201 acacgccaga aacggttgcc gggatgagcg tagttatgtc cagtctcggc cgagcggagg
     1261 gatgggttgt agccgcagat gaccagcgac aggcccggcc gtagcagatc acgaacggcg
     1321 gtgccgtcgc cggacatcag cgggttgagt cgatcagcgt tgccaggcgg tcgggcgtcg
     1381 ccgggtgtgt attgaagagc tgaccgagct tcgggcaacc gaacgggtgg atcaccagca
     1441 ggtgggcgat ggcggggttg acgggccaga cgatccggcc ggccagcgat tgcagtttgc
     1501 gcagcgcgag cgccagcgcc gccgggtcat caatgatccg cgccgcccgc tcgtcggccg
     1561 cgtactcgct ggccggtgag gtcgagatcc ggaccaggat ggcggctatc ggcgccgtta
     1621 gccgggcgat cagatcgcgc agcgggtgca ttctgccgcc gccccagacg cgcatctgcg
     1681 cccgccggta cggcagctcg aaatagaggt cggacccggc cgccgctgtg atcgcgccag
     1741 gaatggaggc gaacagcgcc gcgatcgtcg ctccggcccg gcccggctcg gtcaggtggg
     1801 cgatctcgtg cgccagaacc gcttccagct cggctggcga gagcatcccc agcagcccct
     1861 cggtgacaca gaccagcccg ccgcccggcg tcacggccgc catcgcgttc gggatcggca
     1921 gcgtcgagac ggccacctgc ggcgtcgtaa ttccggccag gtcggcgatc cgacaaacgg
     1981 ccgcgaccag ttgcgggtac tcgccatcag cgaccggcgt cgccgaacag atgcggacca
     2041 ggatcggccg cgagcagaac caggcgaccg tctcaatcag cagcgccacc agcagcccac
     2101 ctaccacacc ccggctgccc gccacgagga agccgccggc catcaatggc gccgcgatga
     2161 cagcttcaag caggaacatc tgccggatag cggtcagccg cgcccccatc acgacagcac
     2221 ctcatagacc ggcagcggcg cgccgaccag cggtcgcgcc cagtcagaga atcgtgatgt
     2281 cacatcgtgg ccagttgcat cgatcatatc gtcatccagc agccgctcaa cgcttgcgat
     2341 ccgttccagc ggcgtcgttc catattccac gtggtagtag ggcgtatcgg cccggcggtt
     2401 gatcgtcacc atcacgcccg tctcgccggc cagcgcacgc cggaccgcct cgcggcccgt
     2461 ttcggcggcc tcgatgaggt cgagctcaga tgcggcgtgc atggccatcc gggcgatcgt
     2521 gccgggcttg tcgtagcggg cgcgtacgcc aagtcgcgcc gacagctccc gcgcaagtgc
     2581 gttgccaacg ccggagaagt atttgtgtcc aaagggatcg atccagtcgg gcgtcgcgcc
     2641 ggcagccgac tgtccgccgg cccacttcat cgtctcgggc acgaccagca cgacgaagcc
     2701 atcgcgggcg atccgctcag tgacgagcgc cgtcagttgc tcgatactct cgaatggccg
     2761 ctccggcagg cagaggacgg gccgtggcaa ctccggctgg acatcgaagg ccaggcttcc
     2821 ggccgccgcc agccagccgg cgttgcggcc catcacctcg atgatcttga cgggatagag
     2881 ctgctgcatc gccagcgtgt cgagggtcgc gtcgcggacg gccagcgcga tgaaacgggc
     2941 gatcgagccg tagccggggc agtggtccat ctccggcaga tcgttgtcga tcgtcttcgg
     3001 gatcgcgacg atccgcaggt cctgcccgcg cgcctgtgcg cgggacgcca gccgcgatgc
     3061 cgtctcggcc gtgtcgttgc cgccgatcgg tgtgaagatc gtgatgttgc gttcctgaag
     3121 gcagtcgagc gtctgctcga cctcggcgtc gctctgccgg tggcggctcg tcccgagcgc
     3181 cgccgacggc gtccgccgca gtcgttcgag acggtcgtca tccagttcgg aaagatcgac
     3241 gaaatcgtcg gtcaggatcc cctcgacgcc cctccgcgcc ccaaggatcc tgccgatctc
     3301 agtcgagcct cgtgcggcct caatgacgcc agccaggctg gcattgatga ccacggtcgg
     3361 cccgcccgac tgggcgacca gcagcgtcgt ggctccgatc tt
//
LOCUS       DASUGJ010000323         3409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_604187, whole genome shotgun sequence.
ACCESSION   DASUGJ010000323 DASUGJ010000000
VERSION     DASUGJ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604187"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VFV93_07250"
     CDS             <1..1126
                     /locus_tag="VFV93_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5165172.1"
                     /translation="SVPESTATAVQRLEDAGAVILGKTNMMEFAYGYPHPDYGETRNP
                     WDTTRTAGGSSGGSAAAVTAGLAYGALGSDTGGSIRSPAAYCGIVGLKPTYGRVSRDG
                     VVPLSWSLDHCGPLTRTARDCAILFDAIAGYDPGDPASARQPLTLTLPDATGDVRGLR
                     VGVVEEFFQRHVEPEVGDIARKAVRVLEGLGVELEPVVFSFIPLIGPTIMPIIQAEAT
                     SYHWKTLLERPEDFGPDIRENLRLGALVLAKDYLDAQRMRRLMAEEVTAALARYDALV
                     FPTQPIVAPPLNAYQVADASDADVLDVEIGHTGLANLTGHPAISVPCGMTSAGLPVGI
                     QFTGRPFDEATIIRLAHTWEQLHDNTSHPPSPVLHPEQET"
     gene            1130..2116
                     /locus_tag="VFV93_07255"
     CDS             1130..2116
                     /locus_tag="VFV93_07255"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX5165173.1"
                     /translation="MNSTDDLSAFPILGPRSRGQRQQHQVPLPYPALADDTWTVTSLT
                     GSEPGPTIFINAGVHGAEYPAIQTIIELGGLLDPARLRGTVVLMPVVNLPGFRQRSMF
                     VCPVDGKNPNRMFPGTPDGSYSQQLAHALMTHFITQAGAHIDLHGGDMVEALIPFSIC
                     QAGESSAAQTSFELATGFGLPYLLVVDRPIQPASGTTTCAAAAAAGIPSFIAEAGGIG
                     LLEPEPVRLLRDGVLRVLGQLGMIDDAPTPAPEPVVLSSFKWLYTPVEGLFYPTVAVG
                     EDVSEGSIVGTVGTLTGEQIAEITAPVSGKVLFLTTSPAMQENGLLMGIGVG"
     gene            2239..>3409
                     /locus_tag="VFV93_07260"
     CDS             2239..>3409
                     /locus_tag="VFV93_07260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165174.1"
                     /translation="MTRPIAFLPETATADARVLVAARTVRAFGDGFVSVLLAAWLKEI
                     GFPSWQFGAITTTTLIGSAALTLAIGLLAWRWPRRRLLLSAALLMALTGLGFSAIHDF
                     WPLLLVAFAGTLNPSSGDVSIFLPLEQSLLPQTVADRERTSLFARYGFAATMSAACGA
                     LFAGVPGFVAERSDLTLKQALMGMFVLYTLLALVALLLYRQLSPAIEPSEAQPAAPLD
                     ESKGTVYRLAALFSIDSFGSGFVVQSLLALWLFERFDISVAAVGTIFFWTGLLSAGSY
                     FIAVRLAQRIGLINTMVFSHLPANLFLMLTPLMPSLPLAIGLLLLRSLLSQMDVPTRN
                     SYVMAVVTPAERPAAASVTAVPRSLATATSPLISGWLLGLTTFGWPLIIGGGLKIA"
BASE COUNT          497 a         1228 c         1095 g          589 t
ORIGIN      
        1 ctcggttccg gagtcgacgg cgaccgccgt ccagcggctg gaggatgccg gcgccgtcat
       61 cctcggcaag accaacatga tggagttcgc ctacggctac ccgcaccccg actacggcga
      121 gacgcgcaac ccgtgggaca cgacccggac ggccggcggc tccagcggcg gctcggcggc
      181 agccgtgaca gccgggctgg cctacggcgc gctcggcagc gataccggcg gcagcatccg
      241 ctcgcccgcc gcctactgcg ggatcgtcgg gctgaagccg acctatgggc gagtcagccg
      301 cgacggcgtc gtcccgctgt cgtggtcgct cgaccactgc ggcccgctga cccggaccgc
      361 ccgcgattgt gcgatcctct tcgacgcgat cgccggctac gatccaggcg acccggcctc
      421 ggcccggcag cccctcacac taacgctgcc cgatgccacc ggcgacgttc gcggcctgcg
      481 ggtcggcgtt gtcgaggagt tcttccagcg tcacgtcgag ccggaggttg gcgatatagc
      541 ccggaaggcc gttagggtgc tggaggggct tggcgtcgag ctggagccgg tcgtgttctc
      601 attcatcccg ctgatcggcc cgacgatcat gccgatcatc caggccgagg cgaccagcta
      661 ccattggaag acactgctgg aacggccgga ggacttcggg ccggacattc gcgagaatct
      721 gcggcttggc gcgctggtgc tggcgaagga ctatctcgac gcccagcgca tgcgccggct
      781 gatggcagag gaggtcacgg cggcgctggc gcgctacgac gcgctcgtct tcccgaccca
      841 gccgatcgtc gcgccgccac tcaatgccta ccaggtcgcc gacgcgtccg acgccgatgt
      901 gctcgatgtc gagatcggcc acaccggcct cgccaacctg accggccacc cggctatctc
      961 ggtgccctgc ggcatgacat cagcgggctt gccggtcggc atccagttca ccggccggcc
     1021 gttcgacgaa gcaacgatca ttcggctggc ccatacctgg gagcagctgc atgacaacac
     1081 ttcccatccc ccatctcccg tgctccatcc cgaacaggag acatgaacga tgaacagcac
     1141 tgacgacctg agcgcgttcc cgattctcgg cccccgctcg cgcggccaac gccagcagca
     1201 tcaggtgccg ctgccctacc ccgcgctggc tgacgacaca tggacggtga catcgctgac
     1261 cggtagcgag ccggggccaa ccatcttcat caacgccggt gtccacggcg cagagtatcc
     1321 agccattcag accatcatcg agcttggcgg tctgctcgac ccggcccggc tacgtgggac
     1381 ggtcgttctg atgccggtcg tcaacctgcc gggcttccgc cagcgcagca tgttcgtctg
     1441 cccggtcgac ggcaagaacc cgaaccggat gttccccggc acgccggacg gcagctacag
     1501 ccagcagctg gcccacgcgc tgatgactca tttcatcacc caggccggcg cgcatatcga
     1561 cctgcatggc ggcgacatgg tcgaggcgct tatcccgttc tcgatctgcc aggccggcga
     1621 gtcgagcgcc gcccagacgt cgttcgagct ggcgaccggc ttcgggctgc cctacctgct
     1681 ggtggttgac cgcccgatcc agccggcttc cggaacgacg acctgcgccg ccgctgccgc
     1741 ggccgggatt cccagcttca tcgccgaggc cggcgggatc ggcctgctgg agccagagcc
     1801 ggtcaggctg ctgcgcgacg gcgttctgcg cgtcctcggc caactcggga tgatcgacga
     1861 cgcgcccacc cctgcccctg aaccggtcgt cctctcctcg ttcaagtggc tctacacgcc
     1921 ggtcgaggga ctcttctacc ccaccgtcgc cgttggcgag gacgtgagcg aaggcagcat
     1981 cgtcgggacg gtcgggacgc tgaccggcga gcagatcgcc gagatcacgg cgcccgtctc
     2041 cggcaaggtg ctcttcctga ccaccagccc ggccatgcag gagaacggcc tgctgatggg
     2101 catcggcgtt gggtgatggg gataggggat gggcggaggg gcatgaggca tgagggtaga
     2161 gaatggctcg gcacgtcttc tcatcactca tcccccatgt atcatgcccc tccgcccatc
     2221 ccccattccc cgccactcat gacccgacca atcgcgttcc tgccggagac ggcaactgct
     2281 gacgcgcggg tcctggtcgc ggcccggacg gtgcgcgcct tcggcgatgg cttcgtctcg
     2341 gtcctgctgg cagcctggct gaaagagatc ggctttcctt cctggcagtt cggcgcgatc
     2401 acgacgacga cgctgatcgg ttctgcggcg ctgacgctcg cgatcggcct gctcgcctgg
     2461 cgctggccgc gccggcggct gctgctcagc gcggccctgc tgatggcgct gaccggactc
     2521 ggattctcgg ctatccacga cttctggccg ctcttgctgg tcgcctttgc cgggacgctc
     2581 aacccgtcgt cgggcgatgt cagcatcttc ctgccgctgg agcagagcct gttgccgcag
     2641 acggtcgctg accgcgagcg cacgtcgctg ttcgcccgct atggcttcgc cgcgacgatg
     2701 tcggctgcct gcggcgcgct tttcgcaggc gtacccggct tcgtcgccga gcggagcgat
     2761 ctgacgctca agcaggcgct gatggggatg ttcgtcctct acacgctgct ggcactggta
     2821 gcgctgctgc tctaccggca actctcgccc gccatcgagc cgagcgaggc ccagccggcc
     2881 gcaccgctgg atgaatcgaa gggaaccgtc taccggctgg cggcactgtt cagcatcgat
     2941 tcattcggca gcggattcgt cgtccagtcg ctgctggcgc tctggctctt cgagcgcttc
     3001 gacatctcgg tcgcggccgt cgggacgatc ttcttctgga ccggcctgct ctcagccggc
     3061 tcgtacttca tcgccgtccg gctggcgcag cggatcggcc tgatcaacac gatggtcttc
     3121 tcgcacctgc cggccaacct gttcctgatg ctgacgccgc tgatgccgag cctgccgctg
     3181 gcgatcgggc tgctgctgct gcgcagcctg ctctcgcaga tggatgtccc aacccgcaac
     3241 tcgtacgtga tggccgtcgt cacgcccgcc gaacggccgg ccgctgccag cgtcacggcc
     3301 gtcccgcgca gcctggcgac ggccaccagc ccgctgatct ccggctggct gctgggcctg
     3361 acgaccttcg gctggccgct catcatcggc ggcggcctca agatcgcct
//
LOCUS       DASUGJ010000324         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_327716, whole genome shotgun sequence.
ACCESSION   DASUGJ010000324 DASUGJ010000000
VERSION     DASUGJ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327716"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            667..1914
                     /gene="rho"
                     /locus_tag="VFV93_07265"
     CDS             667..1914
                     /gene="rho"
                     /locus_tag="VFV93_07265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5165175.1"
                     /translation="MSVSDLDAKQFEELHTIARDLDIANYQRYKKIDLIGRILQAQSE
                     AAGYKYGEGVLDIIEDGFGFLRGERYLPGSDDIYVSQSQIRRFGLRTGDKISGQIRPP
                     KESEKFHSLLRVEAVNGVDPEVARRRPNFDTLTPIFPLELIDLETQPHIISTRLLNLV
                     APIGRGQRGLIVSPPKAGKTIILKAIANGVTSNYQDIHLMVCLIGERPEEVTDMKRSV
                     DGEVVSSTFDEPVEDHTKVAEMVLERAKRLVEGGRDVVILLDSITRLARAYNLAVPPS
                     GRTLSGGIDPIALYPPKRFFGAARNIEGGGSLTIVATCLVDTGSRMDDVIYEEFKGTG
                     NMELHLDRKLAERRIYPSIDIQRSGTRREDLLLDEQSLRQVFTMRRMVSMLGGTEGTE
                     LVLARLAKTPSNAEFLLTLTKDM"
BASE COUNT          392 a          670 c          649 g          297 t
ORIGIN      
        1 tgtctcattt gacggagacg gagcccagcg ttcgattgcc cgggttcgtc cgggccgtgt
       61 gcgtgccgcg ccggcgcgct gttaacagta cggtgagtaa tggagtccca agagcagagc
      121 ggcgatgtcg tgagcgaaca gccggaggcg gcggagaagc cgaagccccg gactcgtgcc
      181 ccacgcaagc gcgcgacgaa ggccacagcc gtggctgaac aaccaactga aacaacagag
      241 tcaacaccgg attcgacggc tggacccatc gagagcgccg ttccggcgat tgaggcaccg
      301 gctgtcgctg caccggaggt gtctgctgca gcggtgagcg cgccgagcgc gcccgccgac
      361 gtggccgaac cagcacccca ggtcgtatcc aacggcgtcg cgaccgagcc ggaggtgccg
      421 gccgaagcgg ctgaagcgcc agtcaaggcg gctgagccgc agcaacgagc gcaacaacca
      481 cagcagcctc gccaggaccg ccagcgtgac cggcgcgacg gcgggcagca tcgtggcgat
      541 tccaatggcg ccagcgatca cccgcgccat caggaccagg gccgagggtt ccgcaaccgc
      601 cagcagcgcg accgggaccg ggaccgcgag cgcaactcgg acctgatcct gcgcgacgcg
      661 ccgctgttga gcgtgagcga cctcgacgcc aagcagttcg aagagctgca cacgatcgcc
      721 cgcgatctgg atattgccaa ctaccagcgc tacaagaaga tcgacctgat cggcaggatc
      781 ctccaggcgc agtccgaggc ggccggctac aagtacggcg aaggcgtgct cgacatcatc
      841 gaggacggct tcgggttcct gcgcggcgag cgctacctgc ccggttccga cgacatctac
      901 gtctcgcagt cgcaaatccg ccgcttcggg ctaaggaccg gcgacaagat ctccggccag
      961 atccgcccgc cgaaagagag cgagaagttc cactcgctgc tgcgcgtcga ggcggtcaat
     1021 ggcgtcgacc ctgaggtcgc gcgccgccgg ccgaacttcg acacgctgac gccgatcttc
     1081 ccgttggaac tgatcgatct cgagacgcag ccgcacatca tctcgacgcg cctgctcaac
     1141 ctcgtcgcgc caatcggccg cggccagcgc gggctgatcg tctcgccgcc gaaggccggc
     1201 aagacgatca tcctcaaggc gatcgccaac ggtgtcacgt cgaactacca ggacatccac
     1261 ctgatggtct gcctgatcgg tgagcggccg gaagaagtca ccgacatgaa gcgctcggtc
     1321 gacggtgagg tcgtctcctc gaccttcgac gagccggtcg aggaccacac gaaggtggcc
     1381 gagatggtgc tggagcgcgc caagcggctg gtcgaaggcg ggcgtgatgt cgtcatcctg
     1441 ctcgactcga tcacgcggct ggcccgcgcc tacaacctgg ccgtcccgcc gagtggccgg
     1501 acactctccg gtggtatcga cccgatcgcg ctctacccgc cgaagcgctt cttcggcgcg
     1561 gcccgcaaca tcgaaggtgg cggcagcctg acgatcgttg ccacctgcct ggtcgacacc
     1621 ggctcccgga tggatgatgt catctacgag gagttcaagg gcaccggcaa catggagctg
     1681 cacctcgacc gcaagctggc cgagcgtcgc atctacccgt cgatcgacat ccagcgctcc
     1741 ggcacccgcc gcgaggatct gctgcttgat gagcagtcgc tacgccaggt cttcacgatg
     1801 cggcggatgg tctcgatgct gggcggcacg gaaggcactg aactggtgct ggcacggctg
     1861 gccaagacac caagcaacgc cgagttcctc ttgacgctga cgaaggacat gtaggtcatt
     1921 cggcgaacaa tcgattcgaa gatgacagga gcggcctcgt gccgctcctg tttgcttgct
     1981 gcgagatcaa tggcaatgca cacaatcc
//
LOCUS       DASUGJ010000325         5712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1300220, whole genome shotgun sequence.
ACCESSION   DASUGJ010000325 DASUGJ010000000
VERSION     DASUGJ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300220"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1441)
                     /locus_tag="VFV93_07270"
     CDS             complement(260..1441)
                     /locus_tag="VFV93_07270"
                     /inference="COORDINATES: protein motif:HMM:NF006569.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEX5165176.1"
                     /translation="MSGRKTADRIGGFGTSVFTEMSRLAVEHQAINLGQGFPDFAGPD
                     FVKEAAAAAIAANMNQYAPSHGTPRLRRAIAADWAARFGREIDVDSEVTVTTGATEAI
                     FVAMLAFLNPGDEIIFFEPFYDAYIADVAIAGASARVVRLHAPNWHFDVDELAAAFSE
                     RTKLLLLNTPHNPTGKVFTRPELEQIAGLCREHDVTVITDEVYDRILFDEHEHVPIAT
                     LPGMWERTLTVNSTGKTFSMTGWKIGFTVGPADLNAAIRAVHQFSTFATATPFQEAMA
                     VALETAEERGYYRQLRDEYSQRRDRLRAVLADAGLPPLDCQGAYFLLSDVTPHGFDND
                     IDFCRYLVTEAGVAAIPPSAFYLDPATAPPLARFCFAKRFETIDAAAERLGRLRQPDS
                     A"
     gene            complement(1556..2143)
                     /locus_tag="VFV93_07275"
     CDS             complement(1556..2143)
                     /locus_tag="VFV93_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX5165177.1"
                     /translation="MAAREPYDQERIIRSSEIHDQPTEPIRRTDVVRRETAPVVDGPV
                     YASDQVVREDVVERPVSRYAYDESAVSENLTIDHVLARRALLDRISSVIWFVAGILEI
                     GIGLRVAFKLLEANGANGFVNFIYDVTEPFVRPFQGIFTDPTSGGAVLDTAALIAMVI
                     WAIVAWAIVRAIWLLLDRPESGVRRSVHEAHRDHA"
     gene            complement(2312..2452)
                     /locus_tag="VFV93_07280"
     CDS             complement(2312..2452)
                     /locus_tag="VFV93_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165178.1"
                     /translation="MSSLAVGLWLLNRDDPFLLTLWGVGLSMLGIVAIVLGLGLLLRI
                     WR"
     gene            complement(2706..4313)
                     /locus_tag="VFV93_07285"
     CDS             complement(2706..4313)
                     /locus_tag="VFV93_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5165179.1"
                     /translation="MSEQVTGGAAAIAALEAAGVEVVFGIPGVHTLALYDALHDRGIR
                     HILARHEQGAGFMADGYARATGKPGVALIITGPGITNVSTPVGEAFADSSPVVVISSQ
                     VERPYAGKMRGNLHDIRDQSGLMGVVTKRSTPVAEFDRIAETVFGAVREATEGRPRPV
                     HVELPLDLLAETGSFEAPSLPSGYRPAPTREQVDQAVELLANATKPLIYVGGGASAAG
                     AAVTELAELLGAPVLCSIMGKGVVAEDHPYGLGHAWNPWGSENPVDDLLTACDVVLVL
                     GSKLGAQETNYWKMPLPATMIRVDIDPGELNLNYPNPTLGIAADAAATADAVLEALRQ
                     HPGVSPRTGIDDVEAFRQRIATERVDEIYYPYIVALREALPRDGIIVQDMTMMSYRMN
                     DAFPSYEPRSYLFPSSYGTLGFSVPAAIGAKIGRPDTPVVAVVGDGGFQFTMQELATA
                     IQFDVTLPIVIFNDSTYTAVEQAMKYEFSGRTMATDLVNPDYVKLADAYGIPGVRAES
                     PTALAEAVQSAFERRGPTIIDVPIPRM"
     gene            complement(4407..5522)
                     /locus_tag="VFV93_07290"
     CDS             complement(4407..5522)
                     /locus_tag="VFV93_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5165180.1"
                     /translation="MAGWTYRCEHCGHQEDLDSRAWRCPRCGGAFGLVGPNTLSPASI
                     TNDPSLWRYESILPARKADGRSLGEGMTPLIAGTLADRPVWFKLDNLLPTGSFKDRGA
                     AVFASHLRRLGLRRLIVDSSGNAAAAFAGYCAANDLDCTVYAPATTSPGKLVQSRAFG
                     AHVHLVEGNREAVASAAQNAAEADPTAFYASHNWHAVFVEGVKTWAIEVWEQLGRRVP
                     AAAFVPTGGGSAFVGGRRGFEAIGKLPALVAAQPAACAPVAAAWNASLNEIPAITPGA
                     TIAEGTKIGAPARSGQILAALRDSGGWAEAVDDALIEATLCDLFRQGMYVEPTAAVSA
                     ATFVSSVKRGAAIPDGDIVILLTGNGLKATDTITSLV"
BASE COUNT          973 a         1822 c         1926 g          991 t
ORIGIN      
        1 cggcgcggtc cactcctgct tgagcacgtg cagatcgctg cgacaaacgc cgctggctgc
       61 gatctggacc agcacctcgc ctgctcgcgg cgggtcgagc tggatagtct cgatcaccat
      121 cggctggttg tactcgtaga agacggcagc acgcatctcg cgcatgggtt cgctccctcc
      181 aatgatctgc cagaggtcgt agatgggaac tcgcgaacga tccggttatg tcgcgcgaat
      241 tgtcgccgtt gtcaagtggc taggcggaat cgggttgtcg cagcctgccg agtcgttcgg
      301 cggcagcgtc gatcgtctcg aaccgcttgg cgaaacagaa gcgcgccagt ggcggcgccg
      361 tcgccggatc gaggtagaac gccgacggcg gaatcgcggc gacgcccgcc tcggtgacga
      421 gatagcgaca gaagtcgatg tcgttgtcga agccgtgcgg cgtcacgtcg ctgagcagga
      481 agtaggcacc ctggcaatcg agcggcggca acccggcgtc ggccagaaca gcccgcagcc
      541 ggtcccggcg ctgcgaatac tcatcgcgga gctgccggta atagccgcgc tcttcggccg
      601 tttcgagcgc gacggccatt gcctcctgaa acggcgtcgc cgtcgcaaag gtcgagaact
      661 ggtggacagc ccgaatcgcg gcgttgaggt cggctggccc gaccgtgaag ccgatcttcc
      721 agccggtcat cgagaacgtc ttgccggtcg agttgacggt caacgttcgc tcccacatgc
      781 cgggcagcgt tgcgatcgga acgtgctcgt gctcgtcgaa gaggatccgg tcgtagacct
      841 cgtcggtgat gacggtaaca tcgtgttccc gacacaggcc ggcgatctgc tccagctcgg
      901 gacgcgtgaa gaccttgccg gttgggttgt gcggcgtgtt caacagcagc agcttcgtgc
      961 gctcgctgaa ggctgcggcc agctcgtcca cgtcgaagtg ccagttgggg gcatggagcc
     1021 ggaccactcg tgccgacgcg ccggcgatcg cgacgtcggc gatgtaggcg tcgtagaacg
     1081 gctcgaagaa aatgatctcg tcgccggggt tcaggaaggc cagcatcgcg acgaagatcg
     1141 cctcggtcgc gccggtcgtg accgtcacct cgctgtcgac gtcgatctcg cggccgaagc
     1201 gcgccgccca gtcggcggcg atcgcccggc gcaagcgcgg cgtgccgtgc gaaggtgcgt
     1261 actggttcat gttggccgcg atcgcggctg ccgccgcttc cttgacgaag tccggcccgg
     1321 cgaaatccgg gaatccctgg ccgaggttga tcgcctggtg ctcgacggcc agccggctca
     1381 tctcggtgaa gaccgacgtt ccaaatccac caatgcgatc cgctgtcttt cgcccgctca
     1441 tgacctgccc ttcccacttc tgacggggca gtatagcgcg caaaagcggg acggctcgat
     1501 ggggagccgt cccgggaaag tggggaaatg tgagagtgcg ttccgtgtga gtagatcagg
     1561 cgtggtcgcg gtgtgcctcg tgaaccgacc ggcggacgcc gctttcgggc cggtccagca
     1621 gtagccagat cgcgcgaacg atggcccagg cgacgattgc ccagatcacc atcgcgatca
     1681 gtgcggcggt gtcaaggacg gcgccgccgg atgtcgggtc ggtaaagatg ccctggaacg
     1741 ggcggacgaa cggctcggtc acatcgtaga tgaagttgac gaagccgttg gcgccgttgg
     1801 cttcgagcag cttgaacgcg acccgcaggc cgatcccgat ctcgaggatg ccggccacga
     1861 accagatcac gctgctgatc cggtcgagca acgctcgccg tgccagaacg tggtcgatcg
     1921 tcagattctc gctgacggcc gactcatcgt aggcgtagcg cgagacgggt cgttcgacga
     1981 cgtcctcacg gacgacctga tcgctggcgt agaccgggcc atcgacgacg ggtgccgtct
     2041 cgcgacggac aacgtcggtg cgacgaatcg gctcggtggg ttggtcgtga atctcgctgg
     2101 atcgtatgat ccgttcctgg tcgtacggct ctcgtgctgc cattgcgcta cctccttcgt
     2161 acaggtgaga tcacctgtcc ctgcggcagc agtaaggcaa gcagcgtgcc acgttcgcgc
     2221 gatacatata tcgcgacacg ctaacgaaca cacagcggct ggacgtaccg gacgggcgtc
     2281 cgccgtcagc tggagcgctc ggtcgatcag ctcagcgcca gatacgcagc agcaggccga
     2341 ggccgaggac gatggcgacg attccaagca tcgagaggcc gacgccccag agcgtcagca
     2401 ggaagggatc gtcgcgattc agaagccaga gaccaactgc cagggatgac atccccgtcg
     2461 cgatccaggt gtccgcgcga ggtatgccgg ctgattcccg aacaggattg tcatccatcg
     2521 gattacgtca cttcccagtc gtgcggagcg cctgcacatg tccgccccgc cgtgacgtgc
     2581 cctgcgagat gtctgattca attctacagg attgcacaga ttgcgcaatc gcgcccgatt
     2641 ggaatagcgc gaactgggcg cgacaactgt cgcgcccagc gaacggttgg ggccgttgac
     2701 gatggttaca tgcgcgggat cggcacgtcg atgatcgtcg ggccgcgccg ctcgaacgct
     2761 gactggaccg cctcggccag cgcggtcggt gattcggcgc gaacgcccgg gatgccgtag
     2821 gcgtcggcca gcttgacgta gtcggggttg accaggtcgg tcgccattgt tcggccgctg
     2881 aactcgtact tcatcgcctg ctcgaccgcc gtgtaggttg agtcgttgaa gatcacgatc
     2941 ggcagcgtca cgtcgaactg gatcgccgtt gccagctcct gcatcgtgaa ctggaagccg
     3001 ccatcaccga cgacggcgac gactggagta tccggccggc cgatcttggc cccgatcgcg
     3061 gccgggaccg agaatcccag cgttccgtag ctggacggga acaggtagct gcgtggctcg
     3121 tacgacggga acgcgtcgtt catccggtag ctcatcatcg tcatgtcctg gacgatgatg
     3181 ccgtcgcgcg gcagcgcctc gcgcagggcg acgatgtacg ggtagtagat ctcatccacc
     3241 cgttcggtgg cgatccgctg gcggaatgcc tcgacatcgt cgattcccgt tcgcgggctg
     3301 actccgggat gctgccggag tgcttccagc acggcgtcgg cggtggcagc cgcgtcggcc
     3361 gcaatgccga gcgtcgggtt cggataattg aggttcagtt cgccggggtc gatatcgacg
     3421 cggatcatcg ttgccggcag tggcatcttc cagtagttgg tctcttgcgc gccgagcttg
     3481 ctgcccagga cgaggacgac gtcgcaggcg gtcagcagat cgtcgaccgg gttttcgctc
     3541 ccccacgggt tccaggcatg gccgaggccg tagggatgat cctcggcgac gacgcccttt
     3601 cccatgatcg agcacagcac gggcgcgccg agcagttcag ctaactcggt aacggccgcg
     3661 ccggcagcac tggcgccgcc accaacgtag atgagtggct tggtggcgtt tgccagcaac
     3721 tcgacggcct gatcgacctg ctcgcgggtc ggcgcgggcc ggtagccaga cggcagcgat
     3781 ggcgcttcga atgaaccagt ctcggccagc aggtcgagcg gcagctcgac gtggaccggc
     3841 cgagggcgtc cctcggtcgc ctcgcgaacg gcgccgaaga cggtctcggc gatccggtcg
     3901 aactcggcga ccggcgtcga ccgcttcgtg acgacaccca tcaggcccga ctggtcgcgg
     3961 atgtcgtgca ggttgccgcg cattttgccg gcgtagggcc gctcgacctg cgaggagatg
     4021 acgacgaccg gcgacgagtc cgcgaacgcc tcaccaaccg gcgttgagac gttcgtgatg
     4081 cccggtccgg tgatgatcag cgcgacgccc ggcttgccgg ttgcccgtgc gtagccatca
     4141 gccatgaagc cggcgccctg ctcgtggcgg gccaggatgt ggcggatgcc gcgatcgtgg
     4201 agcgcatcgt agagcgccag tgtgtggaca cccgggattc caaagacaac ctcgactccg
     4261 gcggcttcga gggcggcgat cgccgctgct ccgccggtta cctgttccga cattccctac
     4321 tccctatcgt cattgtgccg gccgcgatga cgcgggccgt cggtgtgcag cgcaaaggat
     4381 acggtagtgg cgcatctgct tgcccgttac accaacgacg tgatcgtatc ggttgccttc
     4441 aggccgttcc cggtcaacag gatgacgatg tcaccgtccg ggatggccgc tccgcgtttg
     4501 acggacgaga cgaacgtggc ggcactgacg gctgccgtcg gttcgacgta cataccctgg
     4561 cggaacaggt cgcacagcgt cgcctcgatc agcgcgtcgt cgaccgcttc agcccaaccg
     4621 ccggaatcgc gcagggccgc cagaatctgg ccgctacggg ctggggcgcc gatcttcgtg
     4681 ccttcggcaa tcgtcgcgcc gggtgtgatg gctgggatct cgttcagcga ggcgttccag
     4741 gccgcggcga ccggcgcgca ggccgccggc tgggccgcga ccagtgccgg cagtttgccg
     4801 atcgcttcga atccgcgccg cccgccgacg aacgcgctgc cgccaccggt cggaacaaag
     4861 gcggcagccg gtacgcgccg accgagttgt tcccagacct cgatcgccca cgtcttgacg
     4921 ccctcgacga agacggcgtg ccagttgtgg ctggcgtaga acgcggtcgg atcggcctcc
     4981 gcagcgtttt gggcggcaga ggccactgct tcgcgattgc cctcgacgag gtggacgtgc
     5041 gcgccgaacg cccgcgattg cacgagcttg cctggtgagg tcgtcgccgg ggcatagacg
     5101 gtgcaatcca gatcgttggc ggcgcaatag ccggcgaagg cggcagcggc gttgcccgat
     5161 gagtcgacga tcagccggcg cagaccgagt cgccgcaggt gcgacgcgaa gacggcagcg
     5221 ccgcggtcct tgaacgagcc ggttggcagc agattatcga gcttgaacca gaccggccga
     5281 tccgccagcg taccggcgat caacggcgtc atgccctcgc cgaggctgcg gccatcggcc
     5341 ttgcgggccg gcagaatcga ctcgtagcgc caaagcgagg gatcgttggt gatcgacgcc
     5401 ggcgacagag tgttcggccc aaccagcccg aacgctccgc cgcagcgcgg gcaacgccac
     5461 gcgcgagaat cgagatcctc ctggtgaccg cagtgctcac agcgataggt ccagccagcc
     5521 aacgtcatct ccccgcgccc tccaacctcg tcctgtcttt cgtcgccgct tgggagtcta
     5581 gcaggcatcg gctgcacatc acctgcacgc gcggccgttt ccgggtacgc ttctgcctgt
     5641 gactgcgcta acggtgacga tcgaaacccg gttgaaagag ttcatgctgt cactctcgtt
     5701 cgagatggac ga
//
LOCUS       DASUGJ010000326         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_328249, whole genome shotgun sequence.
ACCESSION   DASUGJ010000326 DASUGJ010000000
VERSION     DASUGJ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328249"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VFV93_07295"
     CDS             <1..572
                     /locus_tag="VFV93_07295"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004419 - hydroxymethylglutaryl-CoA lyase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX5165181.1"
                     /translation="DAIALFAAASEAFSQANIRVSIDESFERFNPVAEIATREGIWMR
                     GYVSTAFHCPYSGPVAPAQAIEVAERLFALGCDEVAIADTIGRATEDDTSRLLELAIK
                     RLPVAKLAFHLHDTTGLALANIRLALAAGITIFDGSAGGLGGCPFAPGAPGNLSTEKL
                     VRMLDGMGIETGVDADAVAQAAVEMRELL"
     gene            complement(569..967)
                     /locus_tag="VFV93_07300"
     CDS             complement(569..967)
                     /locus_tag="VFV93_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5165182.1"
                     /translation="MSGTSGRSRPLVLIVEDDRDNAHLIARVLTGEKYDFLVTSVGRD
                     ALHHARTIQPDAITLDLRLPDIDGLDLLAQLKATAETSQIPVIIVSILADERDPRLAQ
                     AFATLTKPIDRERLRETVVSALQSRANCAS"
     gene            1254..2162
                     /locus_tag="VFV93_07305"
     CDS             1254..2162
                     /locus_tag="VFV93_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX5165183.1"
                     /translation="MVATAAKGLHVVPVRYGGSAPNAPDREVVDTYAASADYNVFGES
                     TIREPRFPEDQRDEEPTINLGLIGGEIAKAVAEGRQAGQPVLVVGGNCAITPGVVGGL
                     QQAHGPDAKIGLVWFDAHGDFNTPRTTLSGMLGGMPVAVTAGLAWPRWRELSLQPAPL
                     PTDRIVMVDVRNLDPAEETLVRATDVVIAAPAPGFPGVDLQEAIADLAARCDMIYFHI
                     DSDILDERFVPNHGTKEPNGPDMEQVLAAIDTVMATGKVVAYALVSVWPNGEGGDVAV
                     ASGVEMLKGGLEAWKKHGYPEVAGQG"
BASE COUNT          408 a          755 c          799 g          396 t
ORIGIN      
        1 tcgacgcaat cgcactcttc gcagcggcct ccgaagcctt ctcgcaggcc aacatccgcg
       61 tctcgatcga cgagtcgttc gagcgcttca atccggtcgc cgagatcgca acgcgcgagg
      121 gcatctggat gcgcggctac gtttcgaccg cgttccattg cccctattcc ggaccggttg
      181 cgcctgccca ggccatcgag gtcgccgagc ggctcttcgc gctcggttgc gacgaggtcg
      241 ccatcgccga cacgatcggc cgcgcgacgg aagacgacac cagccggctg ctggagctgg
      301 cgatcaagcg cctgccggtc gcgaagctgg ccttccacct gcacgacacg accgggctgg
      361 cgctggccaa catcaggctg gcgctcgcag ccgggatcac catcttcgac ggctccgccg
      421 gcggcctcgg cggctgccca ttcgcgcccg gcgcccccgg caacctgtca accgagaagc
      481 tcgtcaggat gctcgacggg atgggaatcg agaccggcgt cgacgccgac gccgtcgcgc
      541 aggcggccgt cgagatgcgg gagctgctct aggacgcgca gttcgctcgg ctctgcaagg
      601 cggagacgac cgtctctcgc agtcgctcgc ggtcgattgg tttggtgagc gtcgcgaagg
      661 cttgtgcgag gcgggggtcg cgctcgtcgg cgaggatcga gacgatgatg accgggatct
      721 ggctggtttc cgccgtcgcc ttcagttgcg cgagcaagtc caacccatcg atgtccggca
      781 ggcgcaggtc gagcgtgatg gcgtcgggct ggatcgttcg cgcgtgatgt agcgcgtccc
      841 gaccgactga tgtcacaagg aagtcgtact tctcaccggt caggacgcgc gcgatcaggt
      901 gcgcgttgtc gcggtcgtcc tcgacgatga ggacgagcgg gcgcgagcgg ccggaggtgc
      961 cggacattga cattgaagga ggatcagaag cgccattcga ttgcatagct tcatcgcccg
     1021 gttactgttg aacatcacaa ccggaccgat cccggtgttg tgtcgcaagc gtagtccacc
     1081 gatactgtcg aatggcagcg attccgggtg gttgtgacgc ttctgtgaca ggccggattg
     1141 tcaggtgaat gagtaagacc agccgttttt caggtagcct cacacgaacg atctgccggg
     1201 tgaagcgatg gtacgatggg acggacgttt ccggggaagg taagggagat tcgatggttg
     1261 cgactgccgc aaaggggctc catgtcgttc cggtccgcta cggcggcagc gcgccgaacg
     1321 cgcccgaccg tgaagttgtc gacacctacg ccgcctcggc tgactacaac gtcttcggcg
     1381 agtcgaccat ccgcgagcca cgcttccccg aggaccagcg tgacgaggaa ccaacgatca
     1441 acctcggcct gatcggcggc gagatcgcca aggccgttgc cgagggtcgc caggccggcc
     1501 agccggtgct ggtcgtcggc ggcaattgcg cgatcacacc cggcgtcgtc ggcggcctgc
     1561 aacaggcgca cggtccggac gcgaagatcg gtctggtctg gttcgacgcc cacggcgact
     1621 tcaatacgcc gcggacgacg ctgtcgggca tgcttggggg gatgccggtg gcagtgacgg
     1681 ccggcctcgc ctggccgcgc tggcgcgaac tctcgcttca gccggcgcca ctgccgaccg
     1741 accggatcgt gatggtcgat gtgcgcaacc tcgatccggc cgaggagacg ctggtgcgcg
     1801 cgaccgatgt cgtcatcgct gcgccggccc ctggcttccc cggcgtcgac ctgcaggagg
     1861 cgatcgctga cctggccgcc cgctgcgaca tgatctactt ccacatcgac tccgatatcc
     1921 tcgacgagcg cttcgtcccg aaccacggca cgaaggagcc gaatggcccc gacatggagc
     1981 aggtgctggc agccatcgac acggtgatgg caaccggcaa ggtcgttgct tacgcgctgg
     2041 tctcggtctg gccgaacggc gaaggtggcg atgtcgccgt cgcctcgggc gtcgagatgc
     2101 tcaagggtgg cctggaagcc tggaagaagc acggctaccc ggaggttgcc ggccagggtt
     2161 gatccggcga cggctgggga tacatagcac ttctagatac aatctggcga gaactgacag
     2221 cgactggcct gaacgacgac cgaaagggaa cgaccatagc tgcgatgaca gcgaccgccg
     2281 accagcccat cgtcacgacg tcggccctga ccaagcggta cggcagtgtc gtcgcgctcg
     2341 acaacgtcag cgtgaccg
//
LOCUS       DASUGJ010000327         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1718364, whole genome shotgun sequence.
ACCESSION   DASUGJ010000327 DASUGJ010000000
VERSION     DASUGJ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718364"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFV93_07310"
     CDS             complement(<1..617)
                     /locus_tag="VFV93_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5165184.1"
                     /translation="MAVAAGSDYAVEFRDVSRRFVLHHERRSSFQDWFIGLIKPSGTA
                     EEFWALRDVSFGIRRGETIGIVGRNGAGKSTLLKLVTRILEPTSGKVIVNGRTYAMLE
                     LGAGFHPDLSGRDNIYLNGSLYGFNRKEMARRYEEIVRFAELERFVDTPVKHYSSGMY
                     ARLGFGIAVHMDPEILVIDEVLAVGDANFQQKCFQQLEVLKARGTT"
     gene            complement(619..1398)
                     /locus_tag="VFV93_07315"
     CDS             complement(619..1398)
                     /locus_tag="VFV93_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5165185.1"
                     /translation="MVAQAVELVRYRSLIKNLVAKDLKVRYKSSVLGFFWSLLNPLLM
                     MLVFTFVFTQLLNVTIEHFPVFVLIGLLSWNWTATSVTEGTTSLIDNASLINKVYFPR
                     MLLPISVVASNMANYLLAFPIIFLAMAVTGMAFTPWLLYLPVIVLVQATFLSGLVLIL
                     SAIHVYFRDTTVLVSVGLTAWFFMTPIFFRVEDVRPDLVQWWYWLNPMASIIAELHTI
                     LYHGGVPDPLFMARTLVTSLVLLAIGLLLFGRVSKHLGEHL"
     gene            complement(1690..>3094)
                     /locus_tag="VFV93_07320"
     CDS             complement(1690..>3094)
                     /locus_tag="VFV93_07320"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX5165186.1"
                     /translation="KPLFQLAIVIFLLGSVLSGLSQSMLQLILFRALQGLGAGGLMAL
                     AQAIIGDILSPRERGRYQGYIGSVFAFSSVVGPLLGGLFVDHLSWRWVFYVNVPIGAI
                     ALVVTSIVLKLPVRRRQHSIDYIGSALLVLGIGALLLMTSWGGSEYAWDSPQILALGI
                     GGALAILAFLWQETRAAEPVLPLRLFRERTFAVGSGLGMVVGLAMFGSIAFLPVYFQV
                     VRNASATESGLRMVPMMFGIVSMSIISGRLITKTGKYRIFPIVGTAVMAVGMLLITRM
                     GTDAGQLQTAVSLLVLGVGVGMVMQVIVLAVQNAAPPGDLGIATAGVNLFRSMGSAFG
                     VAIFGAILANRLDVELAKHVPADALQGIDAATLTSSPERLAALPPAVHDGVILAFSNS
                     LHVVFLCALPVVLIGFGLTWLLREQPLRTGPLQGGPTTGEEKQPAQVGAPAALGRPVE
                     AEPRFGDGTPEPVPSLH"
BASE COUNT          651 a         1070 c          912 g          461 t
ORIGIN      
        1 gtcgtcccac gcgccttgag cacttcgagt tgctggaaac acttctgctg gaagttcgca
       61 tcaccgaccg ccagcacctc gtcgatcacc aggatctccg ggtccatatg gacggcaatg
      121 ccgaagccca accgggcgta catccccgac gagtagtgct tgaccggcgt atcgacgaag
      181 cgctccagct cggcaaagcg cacgatctcc tcgtagcgcc gcgccatctc cttccggttg
      241 aacccgtaga ggctgccatt caggtagatg ttgtcgcgcc cggacagatc agggtggaag
      301 ccggccccaa gctccagcat cgcgtaggtg cggccgttga cgatcacctt gccgctggtc
      361 ggctccagaa tgcgcgtcac cagcttcagc agcgtgctct tgccggcccc gttccggccg
      421 acgatgccga tcgtctcacc gcgccggatg ccgaacgaga catcgcgtag cgcccagaac
      481 tcctcggccg tcccgcttgg cttgatcagc ccgatgaacc agtcctggaa ggacgagcgc
      541 cgctcgtggt gcaggacgaa gcgccgcgac acatcgcgga actcgacggc gtagtccgaa
      601 ccagccgcga ctgccatcct acagatgctc cccgaggtgc ttgctcaccc gcccaaacag
      661 cagcagcccg atcgccagca gcaccagcga tgtcaccagt gtccgcgcca tgaacaacgg
      721 gtccggcacg ccgccgtgat acagaatcgt gtgcagttcc gcgatgatcg acgccatcgg
      781 gttcagccag taccaccact ggaccaggtc cggccggaca tcctcgaccc ggaagaagat
      841 cggcgtcatg aagaaccagg ccgtcagccc gacgctcacc agcacggtcg tgtcgcggaa
      901 atagacgtgg atggccgaca ggatcagcac caaccccgac aggaacgttg cctggaccag
      961 cacgatcact ggcaggtaca gcagccacgg cgtgaaggcc atccctgtta ccgccatcgc
     1021 caggaagatg atcgggaacg ccaacaggta gttcgccatg ttcgacgcca cgaccgagat
     1081 aggcagcagc atcctgggga agtagacctt gttgatcagc gatgcgttgt cgattagcga
     1141 ggtcgttcct tcagtaacac tcgtcgccgt ccagttccac gacagtagcc cgatcaggac
     1201 gaacaccgga aaatgctcga tcgtcacgtt cagcagctgc gtgaagacga acgtgaagac
     1261 cagcatcatc aggagcgggt tcagcaacga ccagaagaag cccagcaccg aactcttgta
     1321 gcgaaccttc aggtctttcg ctaccagatt cttgatgagg ctccgatagc ggacgagttc
     1381 gacggcctgc gcgaccaagc tctggatccc ccaacattcc ccgcgcggcc agccccaccg
     1441 gcgccccgcg cgacattgca gtatgaccca ccgcgcccct caacgccaag tacgcagcat
     1501 cacttcgatt gggacgatgc actattcccc ggtaggggcg aggcactgac tcgcccggct
     1561 ccccactcga cgcgtttggg cgggacacga agggcgaggc actgactcgc ccctaccggt
     1621 aacgttgaga atgcaacggc cgggcgtctc gcccggccgt tgtcatcaat catttccgtg
     1681 tgcaccgtct caatgtaacg acggcaccgg ctccggcgtg ccgtcaccaa agcgcggctc
     1741 ggcttcgacc ggccggccca gcgctgccgg cgcgccgacc tgcgccggtt gcttttcctc
     1801 accggtcgtc gggccaccct gtagcggtcc tgtccgtagc ggctgctcac gcagcagcca
     1861 ggtcaggccg aagccgatca gcacgaccgg cagcgcgcag aggaagacga cgtgcagcga
     1921 gttcgagaag gcgaggatca cgccatcgtg gacggccggc ggcaaggccg ccagccgctc
     1981 cgggctggag gtcagcgtcg ccgcgtcgat cccctgcaag gcatccgccg gcacatgctt
     2041 cgcaagctcg acatccagcc ggttcgccag gatcgcgccg aagatcgcca ccccgaatgc
     2101 gctgcccatg gagcggaaca ggttcacgcc ggccgtcgcg atgcccagat cgccgggagg
     2161 cgccgcgttc tggacggcca ggacgatgac ctgcatcacc atcccgacgc cgacacccag
     2221 caccagcaat gagacggccg tctgcaactg gcccgcgtcg gttcccatcc gtgtgatgag
     2281 cagcatccca accgccatca cggctgtccc gacgatcggg aagatgcggt acttgcctgt
     2341 cttggtgatt agccgtccgg agatgatcga catcgagacg attccaaaca tcatcggcac
     2401 catccgcagg ccggactccg tcgccgaggc attgcgcacg acctggaagt agaccggcag
     2461 gaaggcgatc gagccgaaca tcgccagccc gacgaccatc cccaacccgg agccgaccgc
     2521 gaacgtccgc tcacggaaga gccgtagcgg caggaccggt tcggccgcgc gcgtttcctg
     2581 ccagaggaag gccaggatcg ccagcgcgcc gccaattccc agcgcgagaa tctgcggcga
     2641 atcccaggcg tactcgctgc cgccccagct ggtcatcagc agcaaggcac cgatacccag
     2701 caccagcaag gctgaaccga tgtagtcaat cgagtgctgc cgtcgccgca ccggcagctt
     2761 cagcacgatc gatgtcacga ccagcgcgat tgccccgatc ggcacattga catagaacac
     2821 ccagcgccaa ctgagatggt caacgaacag accgcccagc agcgggccaa ctacgctcga
     2881 aaaggcgaag accgagccaa tgtacccctg gtaccggccg cgttcgcgtg gcgagagaat
     2941 gtcgccgatg atcgcctgcg ccagcgccat caggccgccg gcgccgagac cctgcaacgc
     3001 ccggaacaga atcagttgca acatcgactg cgacagaccc gagagaaccg agccgagcag
     3061 aaagatgacg atcgcaagct ggaacagcgg cttg
//
LOCUS       DASUGJ010000328         4988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_467937, whole genome shotgun sequence.
ACCESSION   DASUGJ010000328 DASUGJ010000000
VERSION     DASUGJ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467937"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFV93_07325"
     CDS             complement(<1..496)
                     /locus_tag="VFV93_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5165187.1"
                     /translation="MSRGRKSRAGERGESNTADQQPKRRGSRNAPATDEAAGQRLRTS
                     VQPDELDLQIIALLRVDGRRSNREVARRLGVPEATVRYRVRRLTESGVLKIAASVDPE
                     HLGYALTAVISLEVDPRRFIEASDTIAAMEQVMWLGITTGASDIILTASFKHQDEMFS
                     FVADE"
     gene            complement(730..1218)
                     /locus_tag="VFV93_07330"
     CDS             complement(730..1218)
                     /locus_tag="VFV93_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5165188.1"
                     /translation="MSTSRPRPRFDTSINYYQVLDVSPGASGEEITRAYRGLMRLTHP
                     DNFSEPDQRAKAEERTKLINAAYTVLSKPDIRREYDNQMRATIVSDTLMQRYTGNTPG
                     RRAPAQPGRRPPSAQTARAQRNANRSATLQILFVTGGFVACLIIVIVSVVVTYNALTS
                     LV"
     gene            complement(1320..1904)
                     /locus_tag="VFV93_07335"
     CDS             complement(1320..1904)
                     /locus_tag="VFV93_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165189.1"
                     /translation="MTGHRPAGLGGADLSLLRGRVREVLSLIAEAIPSSELVVVSPLA
                     EGADRLIAREAIDAGYLLDCVLPFPRDDYATDFSTVMARSEYYALLQRASDVIELHGS
                     RDTPELTDAAYLAAGEHVVMHAHLMIAIWDGAGARGSGGTGDVVALALACSLPILWIS
                     ASQPHPLRLVTHDGSGTVEQPLASLAQLVGQYRS"
     gene            2081..4186
                     /locus_tag="VFV93_07340"
     CDS             2081..4186
                     /locus_tag="VFV93_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5165190.1"
                     /translation="MDDQVTVDHRQHPAQPSTPDSILVAGDATVDWMLAVPTVIAPVA
                     LQASYQWESRAAVRIAAQPGGSALVLAVLTACAALEPAGSLQVTGTAVPESALADPAT
                     ENLPRTFTIWQPYPIGAQRRDLTWRMHQFLGVRPAEDGAAVQPSDAAASCLVIDDANL
                     GFRDDPPAWPHCLIDDAAAPGNVILKMSGPLGAGQLWDLLVERFADRLTLYISVGDLR
                     KEFAPIGQPLSWERTASDVTRAVRERSDLALAQRVIVSLGLSGALLIERDGPSRLIFD
                     PLHQEGDWEGARPGTPTGLGTCIAAALALAASRSDDPDWPAAVCSGLAAGRALHEGRL
                     VGPERPDDDGLGFPAAMVARILRDGADSCYRTVAVPENPDWHIFASAIAESHRELAER
                     IVIEGDLAACRDLPVERIGAWSSIGRTEIESMRSVRNIIREFLQERRSRPLSLAVFGP
                     PGSGKSFAIKQMAREWMQGGTQIAVLEFNLSQFADSADLPAALQRVRDCAVEGMLPLV
                     FWDEFDSSLAGRELGWLAQFLAPMQDGAFIEGGIVRPIGPAIFIFAGGTHATMASFKA
                     RAVEIPGAKATDFLSRLRGYVDILGPNPRNADDRTFVLRRALLLRALLQLRAPGLLRG
                     GRLDIDPGVLQAFLDVTTYVHGARSMESIVDMSTLSGRLRYERSALPPRHQLGLHVDP
                     DQFLTLVDARNWQPHPVRP"
     gene            complement(4176..>4988)
                     /locus_tag="VFV93_07345"
     CDS             complement(4176..>4988)
                     /locus_tag="VFV93_07345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5165191.1"
                     /translation="PLLAKAMRRVARKVSAIAPRATSETATPVDESQPPAVVSERFPV
                     LMYHRVSPTGAAHTRRYRVTPEMFESQLAHLREAGYASVSADELLESIELRRSLPGKR
                     VVLTFDDAYVDFAEFAWPLLQRYGFGAFLFVVSGCTGGTNAWDRRYGEELPLLGWDAL
                     RELQAAGVTIGAHTVSHPALTGLSPREIAAEMAESRRTLQEQLGTPIETIAYPYGDQD
                     EIVEHIAGAAGFRLGFTCRLYPLHPHDPPLSLPRVEVEGTDSLETFIAKLTV"
BASE COUNT          760 a         1696 c         1695 g          837 t
ORIGIN      
        1 gctcgtcggc gacgaaggag aacatctcgt cctggtgctt gaacgaggcc gtcagaatga
       61 tgtcgctggc gccggtcgtg atccccagcc acatgacctg ctccatcgcg gcgatcgtgt
      121 cggatgcctc gatgaagcgg cgcggatcga cttcgagcga tatgacggct gtcagcgcgt
      181 agccgaggtg ttcggggtcg accgaggccg cgatcttgag aacgccgctc tcggtcagcc
      241 ggcgcacccg gtagcgaacg gtcgcctcgg gaacccccag ccggcgggcc acctcccggt
      301 tggagcgtcg cccgtctaca cgcaatagcg cgatgatctg caaatccagc tcgtccggct
      361 ggaccgacgt ccgaagtcgc tggccggcgg cctcgtccgt tgccggtgcg tttcgcgatc
      421 cgcgccgttt cggctgctga tcggcagtat ttgattcacc acgctcaccg gcgcggctct
      481 tccgccctct gctcatgccc ctactgtccc gttctgtcgt ggatcgtcag gcgcggccac
      541 ccaatcacgc gccttcctgc atattatccc cgcatttgcg aaagatatcc atccccgtct
      601 ggataatagc atcgtctcac caaaatactt gtcccaggca gtcgtagtgc acacaattcc
      661 ttgcgccgag ttccgcgttt gcggatacca cgtccggcac gaaccggcac cgccagcggg
      721 gatctgcgct cagaccagcg acgtgagcgc gttgtaggtc acgacgacgg agacgatgac
      781 gatgatcagg caggcgacga aaccgccggt gacgaacagg atctggagcg ttgccgatcg
      841 gttagcgttg cgctgggcgc gggcggtctg cgcggatggt ggccggcgac ctggctgggc
      901 cggcgcgcgg cgtccggggg tattgccggt gtagcgctgc atcagcgtgt cggagacgat
      961 cgtcgcccgc atctggttgt catactcgcg ccggatgtcc ggtttcgaga ggacagtgta
     1021 ggcggcgttg atgagcttgg tgcgctcctc ggctttcgcg cgctggtccg gctcgctgaa
     1081 attgtcgggg tgggtcagcc gcatcagccc gcgataggcg cgtgtgatct cctcgcccga
     1141 cgcacccggc gagacatcca gcacctgata gtagttgatc gacgtatcaa accgcggccg
     1201 tgggcgggat gtggacaacg ctcggagtcc tcacccgtgc gcgcaggatt gcgccgtctg
     1261 aaccagcccg cacccatcgg cgggccatcg ggaagtgtag cctatcgcca gaacggctcc
     1321 tacgacctgt actgcccgac cagttgcgcc agcgatgcca gcggctgctc gaccgtgccg
     1381 gatccgtcgt gggtcaccag ccgaagcggg tgcggctggc tggcggagat ccagaggatc
     1441 gggagcgagc aggccagcgc cagcgcgacg acatcgccgg ttccgccgct gccgcgtgcg
     1501 ccggcgccgt cccagatggc gatcatcagg tgcgcgtgca tcacgacatg ctcgccggcg
     1561 gccaggtagg cggcgtcggt cagttccggt gtgtcgcgcg agccgtgcag ctcgatcacg
     1621 tcactcgcgc gctggagcag cgcgtagtat tccgagcgcg ccatcacggt gctgaagtcg
     1681 gtcgcgtagt cgtcacgcgg gaagggcagc acgcagtcga gcaggtagcc tgcatcgatc
     1741 gcctcgcggg cgatcagccg gtcggctccc tccgccagcg gcgagacgac gaccaactcg
     1801 gacgagggga tcgcctccgc gatcagcgac aacacctcgc ggacccggcc gcgcaacagg
     1861 ctgaggtcgg cccctcccag cccggcggga cgatggccgg tcacacctgc ccgaattgac
     1921 ctgggatgcg ttatcgtatc ggtatcagtt ccgtcggttg gctgcggccc cgtcaaggca
     1981 ggctcacttt cgcgattggc cgatggtgca tggcgacgca tgagtcgttg tcgaattgtt
     2041 gcacgacatc gggcagatcc agtgatatcg aggggtaagc gtggacgacc aagtgactgt
     2101 tgaccatcgg cagcatcccg cgcagccgtc gaccccggac agcatcctcg tcgcgggtga
     2161 tgcgactgta gactggatgc tggcggtccc gaccgtgatc gcgccggttg cgctgcaggc
     2221 cagctatcag tgggagtcgc gcgctgctgt ccggatcgcg gcccagccgg gcggctcggc
     2281 gctcgtcctg gccgtcctca ctgcctgtgc cgcgctggag ccggcggggt cgctccaggt
     2341 taccggcacg gcggttccgg agagtgcgct ggctgatccg gcgactgaga acctcccgcg
     2401 caccttcacg atctggcagc catacccgat cggggcccag cggcgtgacc tcacctggcg
     2461 catgcaccag ttcctcggcg tccgacccgc cgaagatggc gccgccgtcc agccatcgga
     2521 cgcagcggcc agctgcctcg tgatcgacga tgccaacctt ggcttccgcg acgacccgcc
     2581 agcgtggccg cactgcctga ttgacgacgc ggctgcgccg ggcaacgtca tcctcaagat
     2641 gtccggtcca ctcggcgcgg gacaactctg ggatctgctc gtcgagcgtt tcgcagacag
     2701 gctgacgctc tacatctcgg ttggcgatct gcgtaaggag ttcgcgccga tcggccagcc
     2761 gctgtcgtgg gaacggacgg cgagcgacgt gacacgggct gttcgcgagc ggtccgatct
     2821 ggccctggcc cagcgggtga tcgtctcgct cggcctgagt ggcgcgctgc tgatcgagcg
     2881 cgacggcccc agccggctga tcttcgaccc gctccaccag gaaggcgact gggaaggcgc
     2941 gcggcccgga acgccaaccg gactgggcac ctgcatcgcc gcagcgctgg cgctggcggc
     3001 ctcccggtcg gatgatcccg actggccggc tgccgtgtgc agcggcctgg cagccgggcg
     3061 tgccctccac gaggggcgtc tcgtcggacc ggagcgcccc gacgacgatg ggctcggctt
     3121 cccggccgcg atggttgccc ggatcctgcg ggacggcgcg gacagctgct accggacggt
     3181 cgcggtgccg gagaaccccg actggcatat cttcgcgtcg gccatcgccg agagtcatcg
     3241 cgaactggcc gaacgaatcg tcatcgaggg cgacctggcc gcctgccgag acctcccggt
     3301 cgagcggatc ggcgcctggt cgtcgatcgg gcgaaccgag atcgagagca tgcgctctgt
     3361 ccgcaacatc atccgcgagt ttctccagga acgtcggagc cggcccctct cgctggcggt
     3421 cttcggcccg ccgggatcgg gcaagtcgtt cgcgatcaag cagatggcgc gcgagtggat
     3481 gcagggcggg acgcagattg ccgtccttga gttcaacctg tcgcagttcg ccgactccgc
     3541 cgatctgccg gccgccctcc aacgggtacg cgattgcgcc gttgagggga tgttgccgct
     3601 cgtcttctgg gacgagttcg acagcagcct ggccggccgc gagcttggct ggctggcgca
     3661 gttcctggcg ccgatgcagg atggcgcctt catcgagggc gggatcgttc gcccgatcgg
     3721 cccggccatc ttcatcttcg ccggcggcac ccacgcgacg atggcgagct tcaaggcgcg
     3781 cgccgtcgag atccccggcg cgaaagcgac cgactttctc agtcgcctgc gtggctatgt
     3841 cgatatcctc ggcccgaacc cgcgcaacgc ggacgaccgc accttcgtcc tgcgccgggc
     3901 gctgctgctg cgggcgctgc tgcaactgcg cgcgccgggg ttgctgcgcg gcggccggct
     3961 cgacatcgac cccggcgtgt tgcaagcctt tctggatgtc accacctacg tgcatggcgc
     4021 gcggtcgatg gagtcgatcg tcgacatgag cacgctctcg ggccggctgc gctacgagcg
     4081 ctcggccctg ccgccccgcc accagctcgg cctgcacgtc gatcccgacc agttcctgac
     4141 cctcgttgat gcccgcaact ggcaaccgca ccccgttaga ccgtgagctt ggcgatgaac
     4201 gtctccaggg agtcggtgcc ttccacctcg acccgtggaa gcgagagcgg cgggtcgtga
     4261 ggatggagcg ggtacagccg gcaggtgaag ccgagccgga acccggcagc tccggcgatg
     4321 tgctcgacga tctcgtcctg atcgccatac gggtaggcga tcgtctcgat cggcgtaccg
     4381 agctgctcct gcaacgtccg gcgggactct gccatctcgg ccgcgatctc gcggggcgag
     4441 aggccggtca gcgccgggtg gctgacggtg tgggcgccga tcgtcacgcc ggccgcctgt
     4501 agctcgcgca gcgcgtccca gccgagcagc ggcagctcct cgccatagcg gcggtcccac
     4561 gcgttcgtcc cgccggtgca gccgctgacg acgaacagga acgcgccgaa gccataacgc
     4621 tgcaacagcg gccaggcgaa ttccgcgaaa tcgacatagg cgtcgtcgaa cgtcagcacg
     4681 acccgcttcc ccggcagcga ccggcgcagt tcgatcgact caagcagctc gtccgcgctg
     4741 acggacgcgt agcccgcctc gcgcaaatga gccagctggc tttcgaacat ctccggcgtg
     4801 acccggtagc gcctggtgtg cgcagcgccg gtcggcgaga cccggtgata catcaggacc
     4861 gggaagcgct cgctgacgac agcgggaggc tggctctcat cgaccggcgt ggctgtctcg
     4921 ctcgtggcgc gcggcgcgat tgccgacacc ttgcgcgcga cgcgccgcat ggccttcgcc
     4981 agcagcgg
//
LOCUS       DASUGJ010000329         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_50399, whole genome shotgun sequence.
ACCESSION   DASUGJ010000329 DASUGJ010000000
VERSION     DASUGJ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50399"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VFV93_07350"
     CDS             <1..710
                     /locus_tag="VFV93_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5165192.1"
                     /translation="RRVEQIARLFDALADDGLDRAGDCEPEAPARIGLWADATLLATE
                     DVRRLVDAALTRAIETGAVVEEAQSPAVYRDLLAIHHITMLVDGAAAHQKLITRYPDA
                     YGPRVRSFVETGMAIPAHSYVRAQILRREARDRALTDWGEFDVIALPTAETPAPGLES
                     TGSPALQAVVTMFGLPSVSLPVGLSPDGLPVGLQLVAPTRLGNARLLRIARWLEGLLE
                     PLPAIPTGIADTVGAPY"
     gene            808..2088
                     /locus_tag="VFV93_07355"
     CDS             808..2088
                     /locus_tag="VFV93_07355"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX5165193.1"
                     /translation="MLELSIKEARRLMLGAQLLAGRPPKRPTKQMMRDTIRHLGALQI
                     DSISVVARSHHIVLWSRLGNHPPEWLDELLGKDRALFEYWAHAAAFVPIEHYPFFRRM
                     MLSFHDGSGTRWSERTREWIEKNRELVDGVIDHIREHGAVSTSTFAAPEGAERAAPWA
                     WYGNKPTNLALEMLWTNGTLMVDRREKFQRWYDLTERVHPTWDDRLIPSVEEERNTLA
                     AVALAALGVSTVRWLPDYFRKNQNDGALRGTTTRNVLEHLAETGFATRARIKGIDDDA
                     YVQTALLDEKIPLSRTTLLSPFDSLVWDRRRAAELFSFPLMLEAYTPAPKRQYGYFSL
                     PILYRDQLVGRLDPKAERKSGQFIVKALHLEPWFAGKDDERFYVSLAANLRDFATFNG
                     CMEFAIERSDPGHAGERLADALGLNVSNDEAAGL"
     gene            complement(2127..>2495)
                     /locus_tag="VFV93_07360"
     CDS             complement(2127..>2495)
                     /locus_tag="VFV93_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX5165194.1"
                     /translation="QSYAPLKIRSAIVPVANAGNLLGPIMVIAGVLIGLTGLAWLGVI
                     FFAAGTLFALVTLPVEFNASSRAMVQLTNIGAVDRTEYGQARKVLNAAALTYVAGFAA
                     ALLNLLYYVMIVTGMGRRND"
BASE COUNT          449 a          839 c          802 g          405 t
ORIGIN      
        1 cgcggcgcgt cgagcagatc gcgcggctgt tcgatgccct ggccgatgac ggactggatc
       61 gcgccggcga ctgcgagccg gaggcaccgg cccggatcgg actctgggcg gacgccaccc
      121 tattggcgac cgaagacgtg cgccgcctgg tcgatgccgc cctgacccgc gcgatcgaga
      181 ccggcgccgt cgtcgaggag gcgcaatcac cggccgtcta ccgcgatctg ctggcaatcc
      241 atcacatcac gatgctggtc gacggggcag ccgcccacca gaagctcatc acccgatatc
      301 cggacgccta cggcccacgc gtgcgctcgt tcgtcgagac cggcatggcg attcccgcac
      361 acagctatgt gcgcgcccag atcctgcgcc gcgaggcccg cgaccgcgcg ctgacggact
      421 ggggcgagtt cgacgtgatc gcgctaccaa ccgccgagac accggcgccc ggtctcgaat
      481 cgacgggcag cccggcgctc caggcggtcg tgaccatgtt cgggcttcca tccgtctcgc
      541 tgccggtcgg tctctcgcct gacggcctgc cggtcggcct ccaactggtc gccccgaccc
      601 gactcggcaa cgcccgcctg ctgcggatcg cgcgctggct cgaagggctt ctcgaaccat
      661 tgccggccat cccgaccggg atcgccgata ctgtcggtgc gccctactga ttcctgcctc
      721 gctcccaacc gcacgaatgt tctactctct gctacaatca gcctcctgca tggtggtgta
      781 cggacacgat cgggaggtca caggacgatg ctcgaactgt cgatcaaaga ggctcgccgg
      841 ttgatgctcg gggcgcagtt gctggccggc aggccgccaa aacgaccgac caagcagatg
      901 atgcgcgaca cgatccggca tctcggtgcg ctccagatcg actcgatcag tgtcgtcgcc
      961 cgcagccatc acatcgtcct ctggagccgg ttggggaacc acccgccaga gtggctcgac
     1021 gaactgctcg gcaaggaccg ggcgctgttc gagtactggg cgcatgcggc cgccttcgtc
     1081 ccgatcgagc actacccgtt ctttcggcgg atgatgctga gcttccacga tggatccggc
     1141 acacgctggt cggaacgcac tcgcgagtgg atcgagaaga accgggaact ggtcgacggg
     1201 gtgatcgacc atatccgcga gcacggcgcc gtctcgacat cgacgttcgc ggcaccggag
     1261 ggcgccgaac gggctgcccc ctgggcgtgg tacggcaaca agccgaccaa cctcgcactg
     1321 gagatgctct ggacgaacgg aacgttgatg gtcgaccggc gcgagaagtt tcagcgctgg
     1381 tatgacctga ctgagcgcgt ccacccgacc tgggacgacc gcttgatccc atccgtcgag
     1441 gaggagcgca acacgctggc ggctgtcgcg ctggccgcct tgggagtctc aacagtgcga
     1501 tggttgccgg attacttccg caagaaccag aacgacggcg ccttacgggg cacgacgacc
     1561 cgcaacgtcc tcgaacatct cgccgagacc ggcttcgcaa ccagggcgcg gatcaagggt
     1621 atcgacgacg acgcttacgt ccagacggcg ctgctcgacg agaagattcc tctcagccgg
     1681 acaacgctgc tctcgccgtt cgacagtctc gtttgggatc ggagacgtgc cgccgagctg
     1741 ttcagcttcc cgttgatgct ggaggcctac acgcctgctc ccaagcgcca gtacggttac
     1801 ttcagcctgc cgatcctgta ccgcgaccag ctcgtcggac ggctcgaccc gaaggcggag
     1861 cgcaagagtg ggcaattcat cgtcaaggcg ctgcacctgg agccatggtt cgccggcaag
     1921 gacgatgagc gcttctacgt gtcgctggca gcgaacctgc gcgatttcgc aaccttcaac
     1981 ggttgcatgg agttcgcgat cgagcgatcg gacccgggcc acgccggcga gcggctcgct
     2041 gatgcgctcg gcctgaacgt cagcaacgac gaagcggcgg ggctgtaagc ctcgccgctt
     2101 cgtcatcacg ttagtagcga tgcacgtcag tcgttgcggc ggcccatgcc ggtcacgatc
     2161 atgacgtaat agagcaggtt gagcagcgcg gccgcgaaac cggccacgta ggtcaaggca
     2221 gcagcgttga gcaccttgcg ggcctggccg tactcggtcc ggtcgacggc gccgatgtta
     2281 gtcagctgca ccatcgcccg cgacgaggcg ttgaactcga ccggcagcgt caccagcgcg
     2341 aacagcgtac cggccgcgaa gaagatcaca cccagccagg cgagcccggt caggccgatc
     2401 aggacaccgg cgataaccat gatcgggccg agcaggttgc cggcgttagc aactggcacg
     2461 atcgccgagc gaatcttgag tggtgcgtat gactg
//
LOCUS       DASUGJ010000330         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1996070, whole genome shotgun sequence.
ACCESSION   DASUGJ010000330 DASUGJ010000000
VERSION     DASUGJ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996070"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /locus_tag="VFV93_07365"
     CDS             <1..1806
                     /locus_tag="VFV93_07365"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165195.1"
                     /translation="IDPCADRPDSDYLIAQAQVNALGAELTQQIVAVDEAQYGPIGEA
                     EPGDPASKSLVMLVYNVLDSFTYDCAATTYTAGYFAPGYIDQYGMNMIVVDALDWASN
                     IGEENGSAIEGIIAHELEHLLMNYSDPGELSWVDEGLADFAAFLNGYYGALTSHLVYH
                     QVFHRDTSLTRWGGTLQNYGASYTFFQYLWTQAGGNGDGNFAQDFDYDGVAGDLLIKL
                     IFEEQADGMDGVQTAIDEFNAQTRADLPSAVELFENWVVAVYLDDENSDTFDINEVDF
                     GSADSLGWTIDIGNEVFYDGRGIYKGAMPDAKWRNFKRVPAQNALPFGTSYEVFRNPG
                     PTFSLTLDGSDTTGVSPHSGDLHWYGGYESQSDNVLNVDAAVAGGNSFDFWSWYFIEE
                     GWDYGFVEALVDGDWVTVPLYDDAGNEVTTNDNPQGNNTEGNGLTGTSGGAYFVDEPQ
                     YIHLNGTVPDGATDLRFRYSTDAAYLDTGWFVDDVSINGVAVDENSDLTTEEGNWVQT
                     NGQQDNNWVAQIIAPCDLTPGATTDGEIVDNAGNYVYRFEGDDISVSGFSTKCLNGPG
                     KKTITVAISNMPGGLINFLDAPYTFRVTNTGNKKK"
     gene            complement(1892..2998)
                     /locus_tag="VFV93_07370"
     CDS             complement(1892..2998)
                     /locus_tag="VFV93_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5165196.1"
                     /translation="MWDVIVVGARCAGATVALRFARSGRSVLLLDKSRAGSDTLSSHV
                     LMPPAIAALDELGLLVDVEALGAPRVHTMLVEFGDRAYPGPVGGQHGFLMSVRRTTLD
                     PLLVDHARRAGATYLAETRVDELVWGDGRVTGVGGRGPGGRLIDERARLVIGADGRHS
                     VVARKVAAEEYNVLVCESGAFYAYLQGLGPTVAGEDAVQFASGPECDILCCPCNEDAH
                     MILLSVSGDEFTDLLAAGSAGFDARIQTVPTLAPRLSGARRVSKVYSATPRELRGFFR
                     HPYGPGWALAGDAGYYAHPASANGIADAFRASRYIHELVERAWVEGSPSETYLDEYRA
                     TRDEESIGPYHFSYRLGKVNPFSDPEIAAAVTGQ"
     gene            complement(3281..>4769)
                     /locus_tag="VFV93_07375"
     CDS             complement(3281..>4769)
                     /locus_tag="VFV93_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5165197.1"
                     /translation="IRVYNTRHEQATSYMADGYARVTGRVGACLVVPGPGLMNALAGL
                     STAYATNSPVLCIAGQIDSQQIGAGRGMLHEIPDQLTMVRSATKWAGRAMSPQEVPAL
                     VRDAFTQLQTGRPRPVEIEIPPNVLQATANVELLDPVAPDRPAGDPDLIERAAQALGQ
                     AQQPLIMAGGGVAGANASATLHELAELLQAPVVVSMGGKGAIDDRSYLAQSMVTGKEL
                     MPSADVILVVGTRFSFPPMAPWGPKPHQTVIQLDVDPEEIGRNYPPQIGIVADANLGL
                     RALVERVPRHNRARTSREQELEELRSRVVDRLFEVQPQASFAGVIRDEMPEDGVVIGG
                     MTQVGYWSVAGFPVYGPRTYVSAGYQGTLGFEYATALGAQVGVPHTRVIAVCGDGGFM
                     YNVQELSTAVAHGINAIAIVFNDGAFGNVRRIQKQQFGGRTIGSDLVNPDFVQLAESF
                     GVDGRRAEGPEALRHQLREALAVDRPALIEVPVGEMPDPWNMLFG"
BASE COUNT          936 a         1555 c         1472 g          806 t
ORIGIN      
        1 atcgatccct gcgcggatcg gcccgacagt gactacctga ttgcccaggc tcaggtcaac
       61 gcgctcggcg cagaactgac acagcagatc gtagccgtcg atgaggcgca gtacggcccg
      121 atcggcgagg cggagccagg cgatcccgcc agcaagtcgc tggtgatgct cgtctacaat
      181 gtgttggatt cgttcaccta cgactgcgcg gcgacgacct acacggccgg ctacttcgcg
      241 cctggctaca tcgaccagta cggcatgaac atgatcgtcg tcgacgcgct cgactgggcg
      301 tcgaacatcg gcgaggagaa cggctcggcg atcgagggca tcatcgctca cgagctcgag
      361 cacctgctga tgaactactc cgatccgggc gagctgtcgt gggtcgatga gggtttggcc
      421 gacttcgccg ccttcctgaa cggctactac ggcgcgctga cctcccacct cgtctaccat
      481 caggtcttcc accgcgacac gtcgctgacc cgctggggcg gcacgctcca gaactacggt
      541 gcctcgtaca ccttcttcca gtatctctgg acgcaggcag gtggcaatgg cgacggcaac
      601 ttcgcccagg acttcgacta cgacggcgtc gccggcgacc ttctcatcaa gctgatcttc
      661 gaggagcagg ctgacggcat ggacggtgtt cagaccgcga tcgacgagtt caacgcgcaa
      721 acgcgcgccg atctgccgtc ggccgttgag ctgttcgaga actgggtggt cgccgtctac
      781 ctcgatgacg agaactccga cacgttcgac atcaacgagg tcgacttcgg ctcggccgac
      841 tcgctgggct ggacgatcga catcggcaac gaggtcttct acgacggacg cggcatctac
      901 aagggcgcga tgccggacgc gaagtggcgc aacttcaagc gcgtcccggc tcagaacgca
      961 ctgccgttcg gcacgtccta cgaagtcttc cgcaaccccg ggccgacctt cagcctgacg
     1021 ctcgacggct ccgacacgac cggtgtttcg ccccacagcg gcgatctgca ctggtacggc
     1081 ggctacgaga gccagtcgga caacgtcctg aacgtcgatg cagccgtcgc cggtggcaat
     1141 agcttcgact tctggtcgtg gtacttcatc gaagagggct gggactacgg attcgtcgag
     1201 gctctggtcg acggcgactg ggtgaccgtt ccgctctacg acgatgccgg caacgaggtc
     1261 acgacgaacg acaacccaca gggcaacaac accgagggga acggcttgac cggcacctcc
     1321 ggcggcgcct acttcgtcga tgagccacag tacatccatc tgaacggcac cgtgccggac
     1381 ggcgcaactg atctgcggtt ccgttactcg accgacgccg cctacctcga caccggctgg
     1441 tttgtcgacg atgtcagcat caacggcgtc gcggtcgatg agaacagcga cctgaccacc
     1501 gaagagggca attgggttca gaccaatggc cagcaggaca acaactgggt tgcccagatc
     1561 atcgccccgt gcgacctaac gccaggcgcg acgacggatg gcgagatcgt ggacaatgcc
     1621 ggcaactacg tctatcggtt cgagggtgac gacatctcgg tgagcggctt ctcaaccaag
     1681 tgcctcaacg gtcctggcaa gaagacgatc acggtcgcaa tctcgaacat gccgggcggg
     1741 ctgatcaact tcctagacgc cccgtacacg ttccgcgtga cgaacacggg caacaagaag
     1801 aagtaacggt ccaggacgaa cgacactgcc ggcccaagtc gggaccggca gtgtcgtcat
     1861 ttgctgaagt ggaggccgac aacgcgaacg gctattggcc cgtcaccgct gcggctattt
     1921 cggggtccga gaacgggttg accttaccca gtcggtagct gaagtggtat ggaccgatgc
     1981 tttcttcgtc gcgagtggcc cgatactcgt cgagataggt ctcggaaggc gatccttcga
     2041 cccaagcgcg ttccaccagt tcgtggatgt acctggatgc acggaacgcg tcggcaatcc
     2101 cgttcgccga ggccggatgc gcgtagtatc cagcgtcgcc ggccagcgcc caaccgggac
     2161 catacggatg ccggaaaaag cctcgtagct ctcgcggcgt ggcggaatag accttgctca
     2221 cccgccgcgc accgctgagg cgcggcgcca gggttggaac tgtctggatg cgcgcgtcaa
     2281 agcccgcgga accagccgcg aggaggtcgg tgaattcatc gccggaaacg ctgagcagga
     2341 tcatgtgtgc gtcttcatta cacggacagc acaagatgtc gcactccggg ccggacgcga
     2401 actggactgc gtcttcgcca gcgacggtcg ggccaagacc ttgtagatag gcgtagaacg
     2461 cgccactctc acagacgagc acgttgtatt cctcagcggc gaccttccgg gccaccaccg
     2521 aatgacggcc gtcagccccg ataaccagcc tggcacgttc atcgatcagc cgaccaccag
     2581 ggccacggcc tccgacgccg gttacgcgac cgtctcccca gaccagttcg tcaacgcgag
     2641 tctctgctag atacgtagcc cctgcgcgcc gcgcgtgatc caccagcaac ggatccagcg
     2701 tcgtccgtct cacggacatg aggaagccat gctgccctcc aaccggaccg ggatacgctc
     2761 tgtcaccgaa ttcgacaagc atggtgtgga cgcgcggagc gccgagtgcc tctacgtcca
     2821 cgagcaaacc caactcatcc aacgccgcga tcgctggggg catgaggacg tgagacgaca
     2881 acgtgtcact tccagcccgc gacttgtcga gcagcaacac tgatcgaccg gaacgggcga
     2941 aacgcaacgc gacggtcgcg cctgcacagc gcgcacccac aacgatgaca tcccacatca
     3001 gcccgcctcc atcatcagat gacgtcgccg ggttgccact ggaagctctc gatccaccag
     3061 cctgaacaac ggcgtgcgac gagatgcgat tccactacct ttgcgttacg gtgaagttct
     3121 gcgcgattat cacacatcgg ggaacagaaa ggggaactgg ttgagcagtg aacgcccgac
     3181 cagttccccg tcgctcttgc gcttccccga aacgctgcct ctgctcgcat cacatttgtc
     3241 gcggagaggc tgatcgctgc cagccgtccg ggatcagcca tcagccaaag agcatgttcc
     3301 atgggtcggg catttcgccg acgggaactt cgatgagtgc ggggcgatcg accgcgaggg
     3361 cttcacggag ctggtggcgc agggcctcgg gcccctcggc gcgccggcca tcgacgccga
     3421 acgattcagc aagctggacg aagtcagggt tgacgaggtc ggagccgatc gtccgtccgc
     3481 cgaactgctg cttctggatg cgccggacat taccgaatgc gccgtcgttg aaaacgatcg
     3541 caatcgcgtt gatgccgtgc gcgacggcgg ttgaaagctc ctggacgttg tacatgaagc
     3601 cgccgtcgcc acagacggcg atcacgcggg tgtgcggcac gccgacctgc gcaccgaggg
     3661 ccgtcgcgta ctcaaagccg agcgtaccct gataccctgc cgagacgtag gttcgtgggc
     3721 cgtaaacggg aaaaccggcg acactccagt agccaacctg agtcatgcca ccgatgacga
     3781 cgccgtcctc cggcatctcg tcccggatca ctccggcaaa cgacgcctgc ggctggacct
     3841 cgaagaggcg atcgacgacc cggctacgga gctcctcaag ctcctgttcg cgcgaggtcc
     3901 gtgcacgatt gtgacgcggt actcgttcga ccagcgcgcg caggccgagg ttggcgtcgg
     3961 caacgatccc gatctgcggc gggtagttcc ggccgatctc ttccggatcg acgtccaact
     4021 ggatgacggt ctggtgtggt ttcggtcccc agggagccat cggcggaaac gagaagcgcg
     4081 tgccaacgac gaggatgacg tcggcggacg gcatcagttc cttgcctgtc accatgctct
     4141 gcgcgaggta gctccgatcg tcgatcgcgc ccttgccgcc catcgagacc acgaccggtg
     4201 cctgcagcag ctcagccaac tcatgcaacg tcgcggatgc gtttgcgccg gcaacgccgc
     4261 caccggccat gatcagcggc tgctgggcct gacccagcgc ctgggcagcc cgttcgatga
     4321 gatccggatc gccggcgggt cgatcggggg caaccggatc gagaagttcg acatttgccg
     4381 tcgcctggag cacattgggc gggatctcga tctcgaccgg acgcgggcga ccggtttgaa
     4441 gctgcgtgaa cgcgtcgcga accagcgccg gcacctcttg cggagacatc gcccggcccg
     4501 cccacttcgt tgccgagcgc accatcgtga gttgatcggg aatctcgtgc agcatgccgc
     4561 gacctgcgcc gatctgctgc gagtcaatct gaccggcaat gcagaggacc ggcgagttgg
     4621 tcgcatacgc cgtcgacaat ccggccagcg cattcattag tcccggcccc ggcacgacaa
     4681 ggcaggcacc aacccgtccc gtgacgcgtg cgtacccgtc agccatgtac gaggtcgcct
     4741 gctcgtggcg ggtgttgtag acgcggatg
//
LOCUS       DASUGJ010000331         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_50928, whole genome shotgun sequence.
ACCESSION   DASUGJ010000331 DASUGJ010000000
VERSION     DASUGJ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50928"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFV93_07380"
     CDS             <1..240
                     /locus_tag="VFV93_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165198.1"
                     /translation="KLSDWDRTGDLGQIEVPTLVIGAQHDTMDPEHMAWMAAALPRGR
                     HLHCPNGSHMAMYDDQEIYVQGLIDFIHDVNAGRF"
     gene            complement(252..1232)
                     /locus_tag="VFV93_07385"
     CDS             complement(252..1232)
                     /locus_tag="VFV93_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX5165199.1"
                     /translation="MKVVFTDPVPADWIQRIQAALPEGASLQAVASYSDEDFGRLAAD
                     ADVLLTMTGRIDAARLAMAPRLRLVQQLGTGYDNIDVEAVRAAGVPAAHNPGANAVAV
                     AEHTIMLMLVLLRQFPACERVTRAGAFARPEGFRTTIELSTATVGLVGLGAIGEAVAE
                     RLAPFGPRLLYTTRHRRDAATESRLRLEYSELPALLAASNVVSIHARLTDATYHLLGD
                     AELARMPAGSYLINTARGGIVDEEALRRAIERGQISGAAFDVLEHEREHVNTFADLPQ
                     VVVTGHMAGAGNASFERMIERSVANVVRAMQGEPVQNLLPELQPGDHLEP"
     gene            1512..>2205
                     /locus_tag="VFV93_07390"
     CDS             1512..>2205
                     /locus_tag="VFV93_07390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165200.1"
                     /translation="MRETDPTMTSTPAVAPPAESPTSALSAEDARRFMLTVCLASVTG
                     PMNFSMLSVALPSIADDLHVSVATGAWLFVVPSLAQSSLQSIGGRLGDLFGYRRMFVL
                     GALGYVIASLLAAAAPVFWALVAGRVLMMVGSSAIQPNTGALVRVHMPAARRASAFGT
                     LVASISLAFAAGPLLGGVLVATIGWRAIFVVAIPVTLTAVLLSQRWVPPDPPGSGNRR
                     SLDVVGAVLWSLT"
BASE COUNT          325 a          782 c          728 g          370 t
ORIGIN      
        1 aagctctccg actgggaccg caccggcgac ctcggccaga tcgaggtgcc aacgctggtc
       61 atcggcgccc agcacgatac gatggacccg gagcatatgg cctggatggc cgcagccctg
      121 ccgcgtggac gtcatctgca ctgccccaac ggcagccaca tggcgatgta cgacgaccag
      181 gagatctacg tccaggggct gatcgatttc atccacgatg tgaacgcggg ccgcttctag
      241 ccgccgcgag gtcagggttc cagatggtcg cccggctgca attccggtag caggttctgg
      301 accggctcac cctgcatcgc acggacgacg ttggcgacgg agcgctcgat catccgctcg
      361 aagctggcgt tgccggcgcc ggccatgtgg ccggtcacga cgacctgggg cagatcggcg
      421 aacgtgttga cgtgctcgcg ctcgtgctcc agcacatcaa aggccgcccc ggaaatctgc
      481 ccccgctcga tggcccgtcg cagcgcctcc tcatcgacga tacctccgcg ggccgtgttg
      541 atcaggtaac tgccggccgg cattcgcgcc agttcagcat cgccgagcaa gtggtaggtc
      601 gcgtcggtca gccgggcgtg aatgctgacc acgttcgagg ccgccagcag tgccggcaac
      661 tcgctgtatt ccagacgcag gcgtgactcg gtcgcggcgt cgcggcggtg acgggtggtg
      721 tacagcaggc gcggcccgaa cggcgccagc cgctcggcga cggcctcgcc gatcgcgccc
      781 agcccgacca gcccaaccgt cgcggtcgat agctcgatcg tcgtccggaa tccctcagga
      841 cgggcaaatg cgccggcgcg cgtcacccgc tcgcaggccg gaaactggcg cagcagcacc
      901 agcatcagca tgatcgtgtg ctccgcgacc gccaccgcat tcgcgccggg attatgggct
      961 gccgggacgc cggccgcccg gacggcttca acatcgatgt tgtcgtagcc ggtgccgagc
     1021 tgctggacca gccgcagacg cggcgccatc gccagccggg ccgcgtcgat ccggcctgtc
     1081 atcgtcagca gcacgtcggc gtcggcagcc agccgtccga agtcctcgtc gctgtagctg
     1141 gcgacggcct gcaacgatgc cccctccggc aacgccgcct gaatgcgctg gatccagtcc
     1201 gccggaaccg gatcggtgaa gacaaccttc acgagccata cccttcatca atatcccgct
     1261 ggacattctc tacgacagtg taaacagatg ccgcggttga tgtgccatgt cgtcctgcgc
     1321 ccagagggca cccgcagcga aggacctctc cgctttgcag tcccttgtgg ctaaggaagg
     1381 ggaggtcctt cgctgcggct caggacgaca gtctcaggtc gcaaggggtt gtgtcacaca
     1441 cccgagtcgg cgatgctgcg ccgtgatccg ttgcctgcca gcgcgactgg caggcggagc
     1501 agtcacgaag cgtgagggag accgacccga cgatgacctc gactccggct gtcgcgccgc
     1561 cggccgaatc accaaccagc gcgctgtcgg cggaggacgc gcgccgcttc atgctgacgg
     1621 tctgtctggc gtcggtcacc gggccgatga acttctcgat gctctcggtc gcgctgccgt
     1681 cgatcgccga cgacctgcat gtctcggtcg cgaccggcgc ctggttgttc gtcgtcccga
     1741 gcctggcgca gtcttccctg caatcgatcg gcggccggct gggcgacctc ttcggctacc
     1801 ggcggatgtt cgtcctcggc gcgctcggct acgtcatcgc ctcgctgctg gcagccgccg
     1861 cgccggtctt ctgggcgctc gtcgccgggc gggtgctgat gatggtcggc agcagcgcca
     1921 tccagccgaa caccggcgcg ctcgtgcggg tgcacatgcc ggccgcccgg cgtgccagcg
     1981 ccttcgggac gctggtcgcc tcgatctcgc tggccttcgc ggccgggccg ctgctcggcg
     2041 gcgtgctggt cgcgacgatc ggctggcgcg cgatcttcgt cgtcgccatc ccggtcacgc
     2101 tgaccgccgt cctgctctcg caacgctggg tgccgcccga cccgccaggc tccggtaacc
     2161 ggcgctcgct ggatgtcgtc ggtgccgtcc tctggtcact gactg
//
LOCUS       DASUGJ010000332         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_885683, whole genome shotgun sequence.
ACCESSION   DASUGJ010000332 DASUGJ010000000
VERSION     DASUGJ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885683"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..860)
                     /locus_tag="VFV93_07395"
     CDS             complement(171..860)
                     /locus_tag="VFV93_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX5165201.1"
                     /translation="MSDDLVWRPERVTVIGLGRFGTSLSRNLHDLGYEVTAIDTSERR
                     VADMANFVSLAAQGDGTDEELLRSLHVDRSDVAIVAQGENLEASVLTTLNLKRLNVAW
                     VVAKARTLQHGDLLARIGADQVIYPEAEAGRRLAHSLAVRHISDYISLTQNSGVANVT
                     APDHFTGHTIAELGTSNYKSLNLLLIKRRRQLLIAPSYDERIEPGDELLLVGPDSDLS
                     AFMDPSRKLDR"
     gene            complement(1059..1718)
                     /locus_tag="VFV93_07400"
     CDS             complement(1059..1718)
                     /locus_tag="VFV93_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5165202.1"
                     /translation="MARAISVLLADDHVLFRQALRHLLEMEFDIKVVAEASDGAAAVA
                     MVEEHRPDVVLLDISMPAMDGIVATARLREEFPDIGIIILTMFAEDTHIIRAIRAGAN
                     GYLLKNTESSRVIESIRAVSRGQSILEPSLVAKLLDEFRKMSDVRAPTNVSGLTDREL
                     ELLRLVARGLSNKEIASTLELAESTVKNRLSILFEKLDVKDRTQAAIYAMSHGLISQP
                     V"
     gene            1980..2666
                     /locus_tag="VFV93_07405"
     CDS             1980..2666
                     /locus_tag="VFV93_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5165203.1"
                     /translation="MLFLFSLRLRLSGGRGEDQHEGEPDAALIERARGGDLPAFNQLV
                     ERHERAVYNVALRYMRSRELAEDVTQEAFLRAWRSLESFRNDAGEGFRAWLLRIASNR
                     ALDVLRAKARRPADSLDRRLDDDESTWEPEGDDEAPLDFAVRDDLGTRLEHALGRLHP
                     DQRLVVILSDIHGHPYDEIAEIAGVPVGTVKSRINRGRARLRDLLLADDGSRELLGAR
                     ARLESGDGRD"
     gene            2729..>3045
                     /locus_tag="VFV93_07410"
     CDS             2729..>3045
                     /locus_tag="VFV93_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5165204.1"
                     /translation="MGDDMRKRSGEVARDASAPPNGSDGNQTMIQPDGHLDAEMVSAW
                     LDAPDDFSEQDQLAIETHLADCSQCRQVAAELTAIVRAFQALPLVETPRPFALADDVA
                     GMRT"
BASE COUNT          523 a          955 c         1042 g          525 t
ORIGIN      
        1 gaacgacgcg gccatgatga cgacgatcag ccccagcgtg aactgcttgg catgtcgccg
       61 tgggttcggg accgactgcc gttcgagggc cggtggcagg ttgatcgtcc gcgccccttt
      121 gacgcggcgg cggaccacgc gatcccccgg cttccggacg ggggactggc tcatcgatcg
      181 agctttcgcg atgggtccat gaaggccgac aggtcgctgt ccgggccgac cagcagcaac
      241 tcgtcgcccg gctcgatccg ctcgtcgtag ctgggggcga tcaacagttg ccggcggcgc
      301 ttgatcaaca gcaggttgag actcttgtag ttcgaggtgc ccagctcggc gatcgtgtgg
      361 ccggtgaagt gatcgggcgc cgtgacgttg gcgacgccgg agttctgggt cagcgagatg
      421 tagtcgctga tatggcgaac cgccagcgag tgcgccagcc gccggccggc ttccgcctcg
      481 gggtagatca cctgatcggc gccgatccgc gccagcaggt cgccatgctg gagggtgcgc
      541 gctttggcga ccacccaggc aacgttgagc cgcttgagat tcagcgtcgt caggacgctg
      601 gcttcgagat tctcgccctg ggcgacgatc gcgacgtccg agcggtcgac gtgcagcgag
      661 cgcaacagct cctcgtcggt gccgtcaccc tgggccgcca gcgagacgaa gttggccatg
      721 tcggcgaccc gtcgctccga ggtgtcgatg gcggtgacct cgtagccgag atcgtgcagg
      781 ttgcgcgaca ggctggtgcc gaagcgcccc aacccgatta ccgtcacccg ctccggccgc
      841 caaaccagat catcgctcac acggtcagcc cttcagcatc gctgcgagtc ggtcgtcaac
      901 acctgcacag tgtacgacgg acgttgcgga gatgctggac gcactataga agtaggggcc
      961 ggtcttgtgc cggccctgct ccacacccga cgcgctcagg gacacgagca gggccggcac
     1021 aaggcacggc ccctacttcc tgggctgatt gctgagtgtt agacaggctg ggagatcagg
     1081 ccgtgggaca tggcgtaaat ggcggcctgg gtgcggtcct tgacgtcgag cttttcgaag
     1141 aggatcgaga ggcggttctt gacggtgctc tcggccagct ccagcgtgct cgcgatctcc
     1201 ttgttggaga gaccgcgcgc cacgagccgc aacagttcca gttcgcggtc ggtcaggcca
     1261 gagacgttgg ttggagcgcg cacgtcggac atcttgcgga actcgtcgag cagcttggcg
     1321 accagcgacg gctccaggat cgactggccg cgcgatacgg cccggatcga ctcgatcacg
     1381 cgggaagact cggtgttctt gagcagatag ccgttagctc cggcccggat ggcgcggatg
     1441 atatgcgtgt cttcggcgaa catcgtcagg atgatgatgc cgatgtcggg aaactcctcg
     1501 cgcagccgcg cggtcgcgac gatgccatcc atggccggca tcgagatatc gagcaggacg
     1561 acatcggggc ggtgttcctc gaccatcgca acagctgccg cgccatcgct ggcttcggcg
     1621 acgaccttga tgtcgaactc catctccagc agatgccgga gtgcctgccg gaagaggaca
     1681 tggtcgtcag cgagtaagac ggagattgcc cgagccatgc tgcctgctac tcctctaagg
     1741 tcctaccggc aatggaaata atgatgctac ctattgcccg tgccgaatcg tttctctgag
     1801 tattgtaatc ggctgggtgg gttgtcgagc atcacacgaa agtcccgaac caacgcagca
     1861 gcgggcgtga cgccgctacg gccgcgcgcc gtcggctata cgttcacctc tataatggtg
     1921 ctattgcgct cgccggggat cggcgtttcg aaggtctcgg gggatacgag gtaggggcag
     1981 tgctgttcct gttcagcctt cgcctgcggc tcagtggtgg ccgtggcgag gaccagcacg
     2041 agggtgagcc ggacgcagcg ctgatcgagc gcgcgcgtgg tggcgatctg cccgccttca
     2101 accagctcgt ggaacggcac gaacgggccg tctacaacgt cgcgctgcgc tatatgcgct
     2161 cgcgcgagct ggccgaggat gtcacccagg aggcattcct gcgcgcctgg cgctcgctgg
     2221 agagcttccg caacgacgcc ggcgagggct ttcgcgcctg gctgctgcga atcgcctcga
     2281 accgggcgct ggatgtcctg cgggcgaagg cacgccggcc ggccgactcg ctcgaccggc
     2341 ggctggacga cgacgagagc acctgggagc cggaggggga tgacgaagcc ccgctcgatt
     2401 tcgccgtccg tgacgacctc gggacacggc tggagcacgc gctcggccgg ctgcatcccg
     2461 accagcggct ggtcgtcatc ctcagcgaca tccacggcca tccatacgat gagatcgccg
     2521 agatcgccgg tgtgccggtc ggaacggtga agtcacgcat caatcgtggc cgcgcccgct
     2581 tgcgcgatct gctactggcg gacgacggca gccgggaact tctgggcgcc cgcgcgcgtc
     2641 tagagagtgg tgacggccgt gactgagccg gcgccacggc caacgatggc ccacggattc
     2701 gatgctgaat tgggagagga gggagtcgat gggcgatgac atgcgcaagc gttcgggcga
     2761 agttgcccgc gacgcctccg ctccgcccaa cggctcggac gggaaccaga ccatgatcca
     2821 acccgacggt catctcgacg cagagatggt ctcggcctgg ctggatgcgc cagacgactt
     2881 cagcgagcag gaccagctcg cgatcgagac ccacctcgca gattgcagcc agtgccggca
     2941 ggttgcggca gagttgacgg cgatcgttcg cgccttccag gcgttgccgc tcgtcgaaac
     3001 accccgaccg ttcgcgctgg cagacgacgt ggcgggaatg cgtac
//
LOCUS       DASUGJ010000333         4551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1163037, whole genome shotgun sequence.
ACCESSION   DASUGJ010000333 DASUGJ010000000
VERSION     DASUGJ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163037"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..2411)
                     /locus_tag="VFV93_07415"
     CDS             complement(357..2411)
                     /locus_tag="VFV93_07415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5165205.1"
                     /translation="MAERIRAIAGWRATLLVVLTTGLVAALTRLAWLAGSPPGFSLAE
                     AQTALAARDLPASDLLRPSPGHEPLFDGLLWLTGTLTGWDVSGARLPAALLGIVTAVA
                     CALWYRRALGPLAGILGGVLAATTFPWLVLSRQAQPAIGAAALAALGLWCLWEGLGPD
                     ADAAGRDRSRRRGWWVVAAGLLVGAGLYTHPTFLPVLVLAPACAALLVWRADPAATPL
                     PSRSWLIAGLACLLLLATPIVVHDLTNPDSFRQRFEQDWDGDGRPEQTANPSAVFSGA
                     GETLASLGWNGHDAATLGTPGRALLDPIMLILAVAGLVVALRHPLAPLHGLTLLWLAG
                     MSVPAALLDPGNPALLMPLTPALFLLPILGLRALVRLAAGKGERPGRLALALAVGAVV
                     ASTGWSLLAYRDWSQADTTYFAFDADLRDALSAIDQPGPGSIPVYIATSPTNAPIISY
                     LHPPGSRPGRTERTIDSRSTIVIPADGIGYLATTTSDPLPDGLTLLLDGQTPADAGST
                     SAGTPAWQIDIAGQTARDRLPWTLPVLGFPDGFALVGFDIRPDLGDVATTGRLPDPPR
                     VLVTLVWNAPRGATPHKARVRLLPVDPAQADTSQAATTAEEWLTTSPPIAAGNRGREL
                     VVIQLSVPVPESPDLIVEVQAGLLRSDGSIQPPTDAGPNAAGDYVLLNRVQYVPDSTT
                     TP"
     gene            complement(2738..2881)
                     /locus_tag="VFV93_07420"
     CDS             complement(2738..2881)
                     /locus_tag="VFV93_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165206.1"
                     /translation="MTASAVCISLDYIRAYPDEIEPAITANAAVEDALVASLTTDGVV
                     TIP"
     gene            complement(2981..3148)
                     /locus_tag="VFV93_07425"
     CDS             complement(2981..3148)
                     /locus_tag="VFV93_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165207.1"
                     /translation="MSRIEIEMDDRRLERLRQVARERGQTPDATAAMFIEEGLRRREF
                     PGIRFRDTGIG"
     gene            3238..3831
                     /locus_tag="VFV93_07430"
     CDS             3238..3831
                     /locus_tag="VFV93_07430"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5165208.1"
                     /translation="MAVEIIYETHSITTDNEAGIATGWLPGQLSEEGRRLAVELGERH
                     RAGDIAAVFVSDLTRAVETATIAFGQTAIPIYQDARLRECNYGTLNGAPVAALAAQRW
                     RRIETPFPGGQSYRQVIDATAEFLADLLAEWDGRRIVIIAHSANRWALECLLGGRAIE
                     DLVDAPFNWQPGWRYTLPAGWKPLRNGDAGPHHPASP"
     gene            3862..>4551
                     /locus_tag="VFV93_07435"
     CDS             3862..>4551
                     /locus_tag="VFV93_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5165209.1"
                     /translation="MELLERGLHLAEFQRLLDGLPEDQGRLVFLGGEAGVGKTALVDA
                     LCGTVRPPVRVLRGGCDPLSTPRPLGPLVDIAGGLGDAVPPLLAEHQPRAFAFRAVLD
                     GLAGRDPALVIFEDVHWADEATLDLLRYLGRRIGPTRALLIATYRDDEVGSNHPLRVV
                     LGDLATAPAIGRRSLAPLSLESVRELAAGSDLDPVRLHRQTAGNPFFVTEVLAAGEIG
                     IPPTVRDAVMAR"
BASE COUNT          734 a         1442 c         1649 g          726 t
ORIGIN      
        1 tcgagttggt cgcgacgacc cgcccctact tgtcgcgtac cctcctcgac aacggcgctt
       61 ggcccttcgc agtagtcagc gatgccgtgg cgtagcggca ggggcgggtc gtcatagcca
      121 gcttgacgca ccgaacgtta agagacccgc ctggcttccc accaccaaac gtgcccatcg
      181 ccacgacgcg catccacgga tggctcgcca ttcccgccat cctcgccctc caacgcagat
      241 tctcaactct ggagacaaca tccatgatga atgaggcgaa ctgaagtcac ccatcagcat
      301 gtgacatatc ccggaaggcg cgcgaaggta gacgagggca cgggttggtg gttggtttat
      361 ggagtggtgg tgctgtcggg gacgtactgg acgcggttga gcaggacata gtccccggcg
      421 gcgtttggtc cggcgtcggt tggcggctgg atgctgccgt cgctacggag caggccggcc
      481 tgaacctcga cgatcaggtc ggggctttcc ggaaccggga cgctcagctg gatcacaacc
      541 aactcgcggc cacggttgcc ggccgcgatc ggtgggctgg tcgttaacca ttcctcggct
      601 gtcgtcgcgg cctggctcgt gtccgcctgg gctggatcga ccggcagcag acggacgcgg
      661 gctttgtgag gcgttgcgcc gcgtggggcg ttccagacga gcgtgacgag cacgcgcggt
      721 ggatcgggga gccgccccgt tgtcgcgacg tcgcccaggt cgggccggat gtcgaagccg
      781 accagcgcga agccgtccgg gaagccgagc accggtagcg tccacggcag ccgatcgcgg
      841 gcggtttgcc cggcgatgtc gatctgccag gctggtgtgc cggcgctggt cgagccggca
      901 tcggccgggg tctgcccgtc gagcagcagt gtgaggccgt ccggcaacgg gtctgaggtg
      961 gttgtcgcca gatagccaat tccgtcggcc gggatcacga tcgttgatcg gctgtcgata
     1021 gtccgctcgg ttctgccggg gcggctgccg ggcgggtgga gatagctgat gatcggggcg
     1081 ttcgtgggcg atgtcgcgat gtagacgggg atcgagccag gcccaggttg gtcgatggcg
     1141 gagagggcgt cgcgcaggtc ggcatcgaag gcgaaatagg tggtatccgc ctggctccag
     1201 tcgcgatagg ccagcagcga ccagccggtg ctggcgacga cggcgccgac cgccagcgcg
     1261 agcgccagcc ggccggggcg ctcgcccttc ccggcagcga ggcggacgag tgcccgcagt
     1321 ccgaggatcg gcagcaggaa gagggccggc gtcagcggca tcagcagggc ggggttgccg
     1381 ggatcgagca acgcggccgg cacgctcatg ccggcgagcc agagcagcgt caggccatgc
     1441 agcggcgcga gcggatgcct gagcgcgacg acgaggccgg cgacggcgag gatcagcatg
     1501 atcgggtcga gcagcgcacg gcccggggtg ccaagggtcg ctgcgtcgtg gccgttccag
     1561 ccgagcgaag ccagtgtctc gcctgcgcca ctgaacacag ccgatgggtt ggcagtctgc
     1621 tccggccggc catcgccgtc ccagtcctgc tcgaagcgtt gccggaaact gtccgggttg
     1681 gtgagatcgt ggacgacgat cggcgtggcg agcagcagca ggcaggccag cccggcgatc
     1741 agccagctcc gtgacggtaa cggcgtggcg gcggggtcgg cccgccagac gagcagtgcg
     1801 gcgcaagccg gtgcgaggac gagcaccggc aggaacgtcg ggtgggtata gaggccggcg
     1861 ccaaccagca agccggccgc gacgacccac cagccccgtc gtctggaacg gtcccggccg
     1921 gctgcgtcag cgtcggggcc gagtccttcc cagaggcacc agaggccgag cgccgccagg
     1981 gcggccgctc cgatcgcggg ctgcgcctgc cggctcagga ccagccacgg gaaggtcgtc
     2041 gcggccagga caccgccgag gatgccggcc agtggaccga gtgcgcgccg gtaccagagg
     2101 gcgcaggcga cggccgtcac gattcccagc agcgcggccg gcaggcgtgc accgctgaca
     2161 tcccaaccgg tcagggtgcc agttaaccag agcagtccat caaagagcgg ttcgtggccg
     2221 ggggaaggac gcagaaggtc gctggcgggc aggtcgcggg cggcgagggc cgtttgcgct
     2281 tcggccagcg agaagcccgg cggggagcct gccagccatg ccagtcgggt cagcgctgcc
     2341 accaggccag tcgtgagcac gaccagcagc gtcgcccgcc agccggcgat cgcgcggatt
     2401 cgttcagcca ttggaactgg ttggtgggat caatccgtcc acggtctgga ggatagacga
     2461 acagaggtcg ccggacaatg ccgcatcggt tggtgcggtg cgaagacacc gggatgatcc
     2521 cgtccgtgtc gaacccaccg gttcgtgagg gctggcacgg tgctggcgtc gcgcgtgatg
     2581 aagacacgac cggctgcagt cgcggctgcc agtatcgttt cgtcactggc agttcgcaga
     2641 tcgccccagt gggcgacgtg aacggcgtcg acgccacttc gtgtcaatgc atcggtgagc
     2701 gcgtgtggca gatgcacatc gagcgcgaca ggcggcatca tggaatagtg acgaccccat
     2761 cggtcgttag cgatgccacg agcgcatctt caacagcggc attggccgtg atcgcaggct
     2821 cgatctcgtc gggatacgcg cggatgtagt cgagtgagat acagactgcg ctagcggtca
     2881 tactgaagtg ctccgcgatc gcatcgatat cgtcgtctac ctcgcgggca agcatggcca
     2941 gtacatagac tggtagtcgt aaacccggca cgaacgcttg tcatccgatg ccagtgtcgc
     3001 ggaaccggat accggggaac tcgcgccgcc gcaggccctc ctcgatgaac atggctgcgg
     3061 ttgcatctgg cgtctggcca cgctcgcggg cgacctggcg caggcgttcc aggcggcggt
     3121 cgtccatctc tatctcaatc ctgctcatgt cgcctcgctt gcgtgccgga tgttgaacgg
     3181 gacgtatcat ggattgtaga cgagtcccac agcatcactg gagaaccgag gtggccgatg
     3241 gcggttgaga tcatctacga gacgcattcg atcacgaccg acaacgaagc cgggatcgcg
     3301 accggttggc tgccgggcca gttgtcggag gaaggtagac ggctggcggt ggagctcggc
     3361 gagcgacatc gcgctgggga catcgccgcc gtcttcgtct ccgatctgac gcgcgccgtc
     3421 gagacggcga cgatcgcgtt tgggcagacc gcgataccga tctaccagga cgcccgcctg
     3481 cgggagtgca actacgggac gctcaacggc gcgccggtcg cggcgctggc cgcccaacgg
     3541 tggcgccgaa ttgagacgcc attcccggga gggcagagct accggcaggt gatcgacgcg
     3601 accgctgaat tcctcgccga tctgcttgcg gagtgggatg gccgccggat cgtcatcatc
     3661 gctcattcgg ccaaccgctg ggcgctggag tgcctgctgg gtggccgcgc cattgaggat
     3721 ctggttgatg cgccgttcaa ctggcagcca ggctggcgct atacgctgcc ggccggctgg
     3781 aagccgctgc gtaacggcga cgccgggccg caccacccag cctcgccgta gccggttgat
     3841 tgccccctct atactggcga tatggaactg ctcgaacgtg gattgcatct ggctgagttc
     3901 cagcgactcc tggacggtct gcccgaagat caggggcggc tcgtcttcct gggcggcgag
     3961 gcgggagtcg gcaagaccgc gctggtcgat gctctatgcg gcaccgtccg tccgccggtg
     4021 cgggtgttgc ggggcggctg cgatccactc tcgacgcccc ggccacttgg gccgctggtc
     4081 gacatcgctg gcgggctggg cgatgcggtc cccccgctgc tggcggagca tcagccgcgc
     4141 gcgttcgcct tccgggccgt cctggatggc ctggccgggc gggatccagc actggtcatc
     4201 ttcgaggatg tccactgggc ggacgaagcg accctcgatc tgttgcgcta tctgggtcgc
     4261 cggatcggcc caacccgcgc cctgctgatc gccacctatc gtgacgacga ggtcgggtcg
     4321 aaccacccgt tgcgggtggt cctcggcgat ctggcgacgg cgccagcaat cggcaggcgc
     4381 agcctggcgc cgctctcgct ggagtctgtc cgcgagctgg cggccgggag cgacctggat
     4441 ccggttcggt tgcatcgcca gacagccggg aaccccttct tcgtcaccga ggtgctggcg
     4501 gcgggtgaga tcggcatccc gccgacggtc cgcgacgcag tcatggcacg g
//
LOCUS       DASUGJ010000334         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1163181, whole genome shotgun sequence.
ACCESSION   DASUGJ010000334 DASUGJ010000000
VERSION     DASUGJ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163181"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VFV93_07440"
     CDS             complement(<1..1010)
                     /locus_tag="VFV93_07440"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX5165210.1"
                     /translation="MDDRSYSLHRDAVVVDAHSDVFCDVVRRRLLGETQVLERLHLPA
                     WRTGGVDVVVTTLYTEEEHKPDRALRRAVTMLGAALNDIAETPDVVLCKTMAEIEAAN
                     AAFKIAFVLAMEGGEPVQDGIESLRVFYELGVRIFGFTWNQRNLLAEGVGEERAGGGL
                     TELGREMVAEANRLGILLDVSHLSVKSFWDMIEVSDAPIIASHSNAKALCGHRRNLDD
                     DQIRALADKGGVIGINGVAAFISDDPKQASLERMLDHLDYIAKLAGVEQVALGPDFVD
                     FLLVGPGAQLHEPIDYPKGFENITTMPAVTAALLDRGYTEDQVRGILGGNMLRLLGTV
                     MG"
     gene            complement(1061..1567)
                     /locus_tag="VFV93_07445"
     CDS             complement(1061..1567)
                     /locus_tag="VFV93_07445"
                     /inference="COORDINATES: protein motif:HMM:NF022167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165211.1"
                     /translation="MMTGADVVKIVSLFEDAGIAFRVDGGWGIDALVGEPTRPHDDLD
                     LVIDRQQNEDAIALLANQGFSMHVDQRPVSFILRAADDRRVDIHPVRFDTDGAGWQAL
                     PDGSEFSYTPEGLTGTGTIAGHTVRCLTAELQVACHLGYEPDGSDIHDMRLLRNRFGV
                     SLPSLYNR"
     gene            1693..>2226
                     /locus_tag="VFV93_07450"
     CDS             1693..>2226
                     /locus_tag="VFV93_07450"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX5165212.1"
                     /translation="MPARTHHIQIDPWTPLGQQEGVGHNRWHEDVPPVLEVDEGDRVV
                     IDTMDAFDTQVAREMSHAGAGQLDLGPVHPLTGPVYVKGAEPGDLLEVRLLDIEADPW
                     GNHGYTAEVPGFGFLRDIFTEPYLVHWDISGDYAESPELPGVRLRYGAHPGVLGLAPS
                     KELRERILAREGALLERG"
BASE COUNT          379 a          756 c          701 g          390 t
ORIGIN      
        1 taccccatca ccgtcccaag cagccgcagc atgttcccgc cgaggatgcc gcgtacctgg
       61 tcctccgtat agccgcgatc cagcagcgcg gcggtgacgg ccggcatcgt cgtgatgttc
      121 tcgaaaccct tcgggtagtc gatcggttca tgcagctgcg cgccgggacc aaccagcagg
      181 aaatcgacga agtcgggacc gagcgcgacc tgctcgacgc ctgccagctt ggcgatgtag
      241 tcgaggtgat ccagcatccg ctccaggctg gcctgcttcg gatcgtcgct gatgaaggcc
      301 gcgaccccgt tgatgccgat cacaccgccc ttgtcggcca gtgcccggat ctggtcgtcg
      361 tccaggttgc ggcgatgccc acagagcgcc ttcgcgttcg agtgcgaggc gatgatcggt
      421 gcatctgata cctcgatcat gtcccagaac gacttgactg agaggtgtga gacatccagc
      481 aggataccga gccggttggc ctcggcaacc atctcccgtc ccagctcggt caacccgcca
      541 ccggcccgct cctcgccgac cccttcggcc agcagattgc gctggttcca ggtgaagccg
      601 aagatgcgca cgccaagctc gtagaagacg cgcaacgact cgatcccgtc ctggaccggc
      661 tcgccgccct ccatcgccag cacgaacgcg atcttgaagg ccgcgttggc ggcttcgatc
      721 tccgccatcg tcttgcagag cacgacatcc ggcgtctcgg cgatgtcgtt gagggccgcc
      781 cccagcatcg tgaccgcccg gcgcagcgcc cggtccggct tgtgctcctc ctcggtgtag
      841 agcgtcgtca cgacaacatc gacgccaccc gtccgccacg ccggcaggtg cagccgttcg
      901 agcacctgcg tctcgcccag caaccgccgc cggacgacat cgcagaacac atccgagtgc
      961 gcgtcaacga ccaccgcgtc ccgatgcagt gaatacgacc gatcatccat cccgccgctc
     1021 cttccaacgc gctgccgatt gcagttcgca ggatagcgcg ctaccggttg tatagcgacg
     1081 gcaggctgac gccgaatcgg ttgcgcagca gacgcatgtc gtggatgtcg ctgccatccg
     1141 gttcgtagcc cagatggcag gcaacctgga gttcggcggt gaggcagcga acggtgtgtc
     1201 cggcgatcgt gccggttcca gtcagccctt caggggtgta gctgaactcg ctgccgtccg
     1261 ggagtgcctg ccagccggcg ccgtcggtgt cgaatcggac cgggtgaatg tcgactcggc
     1321 ggtcatcggc ggcccgcagg atgaagctga ccgggcgctg gtcgacgtgc atcgagaacc
     1381 cctggttggc cagcagtgcg attgcgtcct cgttctgctg tcggtcgatg accagatcga
     1441 ggtcatcgtg cgggcgcgtc ggttcgccaa cgagtgcgtc aattccccag ccgccatcga
     1501 cccggaaggc gatgcctgcg tcttcgaaca ggctgacgat cttgacgaca tcagcgccgg
     1561 tcatcatcgc gtcaccacat cgccatgatc cagaaacggg taaccccatt cgctacccca
     1621 tgaaccatcc atctcgaacg tgcgctacac tgcccgcaac cgactacagc acattggcga
     1681 agggagctgg tcatgcctgc caggacgcat catatccaga tcgacccgtg gacgccgctg
     1741 gggcagcagg agggtgttgg ccataaccgc tggcacgagg atgttccgcc cgtgctggag
     1801 gtcgatgagg gtgaccgcgt cgtcatcgac acgatggacg ccttcgatac ccaggttgcg
     1861 cgcgagatgt cgcacgccgg cgccggacag ctcgatctcg gtccggtcca tccgctgaca
     1921 ggcccggtct acgtcaaggg cgccgagccc ggcgatctgc tggaggtccg gctgctcgat
     1981 atcgaggccg atccgtgggg caaccacggc tacacggccg aggtgcccgg cttcggcttc
     2041 ctgcgcgaca tcttcaccga gccgtatctc gtgcattggg acatcagcgg cgactacgcc
     2101 gaatcacccg agttgcccgg cgtgcgcctg cgctacgggg cgcatccggg cgtgctcggg
     2161 ctggcgccgt cgaaggagtt gcgtgagcgg attctcgccc gcgagggtgc cttgctggag
     2221 cgtggc
//
LOCUS       DASUGJ010000335         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_746280, whole genome shotgun sequence.
ACCESSION   DASUGJ010000335 DASUGJ010000000
VERSION     DASUGJ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746280"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFV93_07455"
     CDS             <1..346
                     /locus_tag="VFV93_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165213.1"
                     /translation="DALAIGTTSTTTTYNFDVLSAGEILDNLSALTEELVTNPGAERA
                     LLAALTRAQTFADAGNPFMAYISMLQYVMLLNRFDRTGIVSDDAAQQLYALALDATRA
                     ILTPPAPPSTPK"
     gene            446..1390
                     /locus_tag="VFV93_07460"
     CDS             446..1390
                     /locus_tag="VFV93_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX5165214.1"
                     /translation="MTNAATTAIVWSETYQDHDTDSHPESPERIVALGRALRAAGMFD
                     DRSVLTPEPATINAILAVHAPQIVDLVQRAAQRGGAWLDPDTYVSPDSYEVALLAAGG
                     ACRAVDAVMTGEAQRAFAVVRPPGHHAEPDRSMGFCLFNNIAVAAEHALNAYGLERVA
                     IVDWDVHHGNGTQAAFYRRPDVLFVSLHQYPFYPGSGAAAERGAGAGEGYTINIPLPA
                     GSGDEVYATRFSDTVLPALHAFAPELILVSAGFDAHIDDPLAMMRVSTDAFAGWARHI
                     KQAAATLCDGCLALILEGGYNLRALGESVVAVLRALDE"
     gene            1572..>2098
                     /gene="hisH"
                     /locus_tag="VFV93_07465"
     CDS             1572..>2098
                     /gene="hisH"
                     /locus_tag="VFV93_07465"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX5165215.1"
                     /translation="MNPENKIVVVDYGAGNVQSVVNALDALGIEAELTTDASTIRQAD
                     GIIVPGVGAAQDTIGNLERRGLVEPVLEIIERDMPYLGICMGMQALMSSSDENGGQPC
                     LDVIPGVGRRFETTLPVPHMGWNQVQRTPAGSRHPIFDDIPDGAEFYYVHSYYCAPTD
                     PGWVLAETDYDGRFA"
BASE COUNT          343 a          744 c          661 g          350 t
ORIGIN      
        1 cgatgcgctg gcgatcggca caaccagcac gacgacgacc tacaacttcg atgtgctctc
       61 ggcgggcgag atcctcgaca acctcagcgc attgacggaa gaactggtga ccaacccggg
      121 cgccgagcgt gccctgctgg ccgcgctcac ccgggcccag acatttgctg acgccggcaa
      181 cccgttcatg gcctacatct cgatgctgca gtacgtcatg ctgctcaacc gcttcgaccg
      241 gaccggtatc gtcagcgacg atgccgccca gcagctctat gcgctggccc tcgatgcgac
      301 gcgcgcgatc ctgacgccgc ctgcgccgcc cagcacgcct aagtaagttt cgcgccttct
      361 ggcatctcac gccggcgacc ggcggcccca ccgtaacggg gccgctggtc atttccgggc
      421 gaccggtggc gggtatactc gctccatgac gaatgcagca acgacggcca tcgtctggtc
      481 tgagacctac caggatcatg acaccgactc gcatccggaa tcgccggagc ggatcgttgc
      541 ccttggccgc gcgctgcggg cggccgggat gttcgatgac cgcagcgtcc tgacgccgga
      601 gccggccacg atcaacgcga ttctggccgt ccacgctcca cagatcgtcg atctcgtcca
      661 gcgggcggcc cagcggggcg gcgcctggct cgaccctgac acctacgtct cgccggattc
      721 gtacgaggtc gcgctgctgg cagccggtgg cgcctgccgg gccgtcgatg ccgtcatgac
      781 cggcgaggcc cagcgcgcct ttgccgtcgt ccgcccgccg gggcaccacg ccgagccgga
      841 ccggtcgatg ggcttctgcc tgttcaacaa catcgccgtc gccgccgagc acgcactcaa
      901 cgcctacggc ctcgaacggg tcgccatcgt cgattgggat gtccaccacg gcaacggcac
      961 ccaggccgcc ttctaccgcc ggccggatgt gctcttcgtc tcgctgcacc agtacccgtt
     1021 ctaccccggc agcggcgcgg ccgccgagcg gggcgccgga gccggcgagg gctacacgat
     1081 caacatcccg ctcccggccg gcagtggcga cgaggtctac gcgacccgct tcagcgacac
     1141 cgtcctccca gccctgcacg ccttcgcccc ggagttgatc ctcgtctcgg ccggcttcga
     1201 cgcccacatc gacgacccgc tcgcgatgat gcgcgtctcg accgacgcct tcgccggctg
     1261 ggcacgccac atcaagcagg ccgccgccac cctctgcgac ggctgcctcg ccctcatcct
     1321 cgaaggcggc tacaacctgc gggcgctggg ggagagcgtg gtcgcggtgt tgcgggcgtt
     1381 ggatgagtga cgaaagcggg aaacgggatt cggtattcgg gggtatccaa ctttggatat
     1441 ccacgaagaa catgccaagt ctctcagccc cgaatcccga atcccgtttc ccgaccagcc
     1501 gtcccgcatg attcaattac gcccatacac ggtcgccgcg tgttgtggaa ccattggacg
     1561 gctgaacctc gatgaaccca gagaacaaga tcgtcgttgt tgattacggc gccgggaatg
     1621 tccagagtgt cgtcaatgcg cttgatgcgc tcggcattga ggcggagctg acgaccgacg
     1681 cctccacgat ccggcaggcg gatgggatca tcgtgccggg tgtcggggcc gctcaggaca
     1741 cgatcggcaa cctggagcgg cgcgggctgg tcgagccggt gctggagatc atcgagcgcg
     1801 atatgcccta cctcggcatc tgtatgggca tgcaggcgct gatgtcgtcg tccgatgaga
     1861 acggcggcca gccctgcctg gacgtgatcc ccggtgttgg gcggcgtttc gagacgacgc
     1921 tgccggtgcc gcacatgggc tggaaccagg tccagcggac gccggccggc agccggcatc
     1981 cgatcttcga cgatatcccc gacggcgccg agttctacta cgtccactcc tactactgcg
     2041 cgccaaccga ccccggttgg gtgctggctg agaccgacta cgatggccgc ttcgcctg
//
LOCUS       DASUGJ010000336         4268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1163333, whole genome shotgun sequence.
ACCESSION   DASUGJ010000336 DASUGJ010000000
VERSION     DASUGJ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163333"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFV93_07470"
     CDS             complement(<1..917)
                     /locus_tag="VFV93_07470"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX5165216.1"
                     /translation="MSTIQATSAVTTGRTFPFYRFNGTHRQIGEQFGEACRELIHLHR
                     ETVLYRLGSRNGLTRERALDKAMGYRHYVSQYAAFFDDEIQGLADAAGLSLPEAYLLQ
                     LRAEAATPVIDDETQECTTFAVQPEATANGIGLVGQNADLPAFYRDIAITAEIRADDQ
                     PAVLMVLPAGQVSYIGINDRGVGVFANFVTCDGWRKGFPRYFLSRLALTEDSVDGAID
                     RVRSVRRASSRNLIMLDANGTAADLETTPTRDARLEPEDGLLAHSNHYVHESLHDEER
                     SPEKNVANSKIRLQQMRDLLAENRGQLDA"
     gene            1007..2701
                     /locus_tag="VFV93_07475"
     CDS             1007..2701
                     /locus_tag="VFV93_07475"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX5165217.1"
                     /translation="MPITMERSEYNRRFGATTGDRVRLADTNLFARIERDDSLPGFEP
                     VAGFGRPLRDGMLFGRSSLSQLDLVITNVIVIDPVLGIFKSNIGIKDGRIEGIGRAGN
                     PDVTDNIDVVIGSATGIIPGDGLIATPGGVDSHVHLSSTSLIPTALYSGITTLVAQGS
                     GGVWDLGVNPINNMRHIFEAFEAIPINLAILARGSSDREQLAAHFEAGASGLKIHEDV
                     GAFPAVIDACLTVADQAGGQVAIHTDGLNEAGALSETIAAIDGRSIHAYHVEGSGGGH
                     APNLIEVCSVSNVIGSSTNPTLPYSVNSVAEQLDMVIAVHRLDRQNPEDMAAARDRVR
                     AGTIAAEDVLHDLGAIAIVSSDSQGMGRIGQVISRTWQVAHRMKELRGGGFDEATGEG
                     DDNPRIIQYVAKTTINPAIAHGLDHEVGSIEPGKLADIILWHPAFFGVKPQTIVKGGF
                     VVASQGSDGDGSTRTSQPLIYRPMFGSYGLAPARLAVNFVSSQAANGIPNSTHLIRRS
                     VHIRNVRTTFKRDMLHNTASPDVRVDPRTAEVYVDDELVANPPAETLPLTQRYFLG"
     gene            2742..3098
                     /locus_tag="VFV93_07480"
     CDS             2742..3098
                     /locus_tag="VFV93_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165218.1"
                     /translation="MTSGKTPELVTPLLELAHSASQDGPAWSQTTDDLNVNLIVLRAG
                     HSIAEHINAEVDVLLVGIEGEGSVTIDGSHHDFASGQSVIIPKGAARSITPRGARFAY
                     LTCHKRRAGLWPKPRT"
     gene            complement(3312..>4268)
                     /locus_tag="VFV93_07485"
     CDS             complement(3312..>4268)
                     /locus_tag="VFV93_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5165219.1"
                     /translation="ELGRIDTSHQVSLTVHLGLASMCILQWGSEAQREQWLPALARGE
                     RIGTFGLTEPGAGSDVGALRMRAREVEGGYLLNGEKSWISSANVANLFLLFATVDPAK
                     RHRGITAFVIPGESPGLTTTKLHGKLGVRAGDTGTVVCNDVFVPRENVLGEVGEGFAV
                     ALSALGNGLFTVGCGALGIAAACREWTADYLRALGNTGSGWEAAELAEMVAREQTSRS
                     LLAQAAALKDAGQPNTRQTGNAKWTASEAGYANAQAALAIYNAHEAGDNLTLARHWAN
                     AKGAVIYGGTSEIHQTMQAAYVLGDRIERPFRIPNPAAADLA"
BASE COUNT          746 a         1442 c         1371 g          709 t
ORIGIN      
        1 tcggcgtcga gctggccgcg attctcggcc agcaaatcgc gcatctgctg gagccggatc
       61 tttgagttgg cgacgttctt ctccggagag cgctcctcgt cgtgcagcga ctcgtggacg
      121 tagtggttgg aatgcgccaa caggccatcc tccggttcga gccgcgcgtc gcgggttggt
      181 gtcgtctcca gatcagcggc cgtgccattg gcatcgagca tgatcaggtt gcgcgaggat
      241 gcacggcgga cggagcggac ccgatcgatc gcaccatcga cgctgtcctc ggtcagcgcc
      301 agccgcgaga ggaaatagcg cgggaagccc ttgcgccagc catcgcaggt cacgaagttg
      361 gcgaacacgc ccactccgcg gtcgttgatg ccgatgtacg agacctgtcc cgccggcagc
      421 accatcagga cggccggttg atcgtcggca cggatctcgg ccgtgatcgc gatgtcgcgg
      481 tagaaggccg gcaggtccgc gttctgtcca acgagcccga taccgttggc ggtcgcttcc
      541 ggctgaacgg cgaacgttgt gcattcctgc gtctcgtcgt cgatcacggg cgtcgccgct
      601 tcggcgcgca gttgcagcag gtacgcctcc ggcaagctca ggccggcggc atccgccaag
      661 ccctgaatct catcatcgaa gaaggcggcg tactgactga catagtggcg gtagcccatc
      721 gccttgtcga gcgcccgctc gcgcgtcaga ccgttccggc taccaaggcg gtagaggacc
      781 gtctcacggt gcaggtggat cagctcgcga caggcttcgc cgaactgctc gccgatctgc
      841 cggtgcgtgc cgttgaagcg gtagaacgga aaggttctgc cggtagtcac tgccgatgtc
      901 gcctggatcg tgctcatctg tctcccccga tcgcgtctgc tggctgcaca ttgccgccag
      961 cgtatcatgg cttgcggtaa cctgagcggt aacgaaggga cgccggatgc cgattacgat
     1021 ggagcgctcc gaatacaacc gccgcttcgg cgcgacgacc ggcgaccgcg tccggctggc
     1081 cgacaccaac ctcttcgcgc ggatcgagcg cgacgacagc ctgccaggct tcgagcctgt
     1141 cgccggtttc ggcaggccgc tacgcgacgg gatgctgttc ggccgctcca gtctcagcca
     1201 gctcgacctc gtcatcacca acgtgatcgt catcgatccc gtcctcggca tcttcaagtc
     1261 caacatcggc atcaaggatg gccggatcga gggcatcggc cgggccggca acccggatgt
     1321 gactgacaac atcgacgtcg tcatcggcag cgcgaccggc atcatccccg gcgacggcct
     1381 gatcgcaaca ccgggcggcg tcgacagcca cgtccacctg tcatcgacca gcctgatccc
     1441 gactgccctc tactccggca tcacgacgct ggtcgcgcag gggagcggag gtgtctggga
     1501 cctcggtgtc aacccgatca acaacatgcg ccacatcttc gaggcgttcg aggcaatccc
     1561 aatcaacctg gcgatcctcg cgcgcggctc cagcgaccgc gagcaactcg ccgctcattt
     1621 cgaggccggc gcgtcggggc tgaagatcca tgaggatgtc ggcgcgttcc cggccgtgat
     1681 cgacgcctgc ctgacggtcg ccgatcaggc aggcgggcag gtcgccattc acaccgacgg
     1741 cctgaacgag gccggggcgc tgtccgagac gatcgcggca atcgacgggc gctcgatcca
     1801 cgcctaccac gtcgaaggct ccggtggcgg ccacgcaccg aacctgatcg aggtctgctc
     1861 ggtttcgaac gtgattggct cgtcgaccaa cccgaccctg ccctacagcg tcaactcggt
     1921 cgcggaacag ctcgacatgg tcatcgccgt ccaccggctc gaccggcaga atcccgaaga
     1981 catggcagcc gcccgcgacc gcgtccgggc cggaacgatc gccgccgaag atgtactgca
     2041 cgacctcggc gcgatcgcga tcgtctcttc cgactcgcag gggatgggca ggatcgggca
     2101 ggtcatctcg cgcacctggc aggtcgccca ccgcatgaag gagctgcgcg gcggcggctt
     2161 cgacgaagcg acaggcgaag gcgacgacaa cccgcgcatc atccagtacg tcgccaagac
     2221 gacgatcaac ccggccatcg cccacgggct cgaccacgag gtcggcagca tcgaaccggg
     2281 caagctggcc gacatcattc tctggcatcc agccttcttc ggcgtcaagc cgcagacgat
     2341 cgtcaagggc ggattcgtcg tcgcctcgca gggcagcgat ggagacggtt cgacgcgcac
     2401 gtcgcaaccg ctgatctacc ggccgatgtt cggcagctac ggtctcgccc cggcccggct
     2461 ggccgtcaac ttcgtctcgt cgcaggccgc caacggcatt cctaacagca cgcatctgat
     2521 ccgccggtcg gtacacatcc gcaacgtccg gacgacgttc aagcgcgaca tgctgcacaa
     2581 cacggccagc cctgacgtgc gggtcgatcc ccgaacggcc gaggtgtatg tcgatgacga
     2641 gctggtcgcc aatccgccgg ccgagacgtt gccgcttacg caacgctatt tcctgggttg
     2701 aatcatgatg cacgcgagtt ctcgattgaa gtggggttga tatgacaagt ggcaagactc
     2761 cggaactggt cacgccattg ctggagctgg cgcattcggc ctcgcaggac ggaccggcct
     2821 ggtcgcagac gactgatgac ctgaacgtga acctgatcgt cctgcgggca gggcactcaa
     2881 tcgccgagca catcaacgct gaggtcgatg tcctgctggt cggtatcgaa ggcgagggca
     2941 gcgtcacgat cgacggcagc catcatgact ttgcatctgg acagtcggtc atcatcccga
     3001 agggagcagc ccgctcgatc actccgcgcg gcgcccgctt cgcctacctg acctgccaca
     3061 aacgccgtgc tggcctctgg cccaagccac ggacgtgatg agcctcggca accagccgcg
     3121 ctgccctcga cactgaggcg gcgtgaagag gatgagcagt tcccctgtcg tcctgagcca
     3181 cagcgaagga cctctctttc cgaaccgccc accatgttga cgcggagagg tccttcgctg
     3241 tggctcagga cgacagggga aggggttcat cgcggggaac acagccggcg cgaatacgtg
     3301 atttagaacg ctcaggccaa atcagccgcc gcggggttcg gaatgcggaa cgggcgctcg
     3361 atgcggtcgc cgaggacgta ggcggcctgc atcgtctggt ggatctcgga cgttccgccg
     3421 tagatgacgg cgcccttcgc gttggcccag tggcgcgcca gcgtcaggtt gtcgccggct
     3481 tcgtgggcat tgtagatcgc cagcgcagcc tgggcgttgg catagccggc ctcggaggcg
     3541 gtccacttgg cgttaccggt ctgacgcgta ttgggctggc cggcgtcctt gagggcggcg
     3601 gcctgcgcca gcagcgaccg ggaggtctgc tcgcgggcga ccatctcggc cagttcggca
     3661 gcctcccaac cggagccggt gttgccgagc gcccgcaggt aatcggcggt ccactcccgg
     3721 caggcagccg cgatccccag cgcgccgcag ccgacggtga agagcccgtt gcccagcgcc
     3781 gagagggcaa ctgcgaaccc ttcgccaacc tcacccagca cgttttcgcg tggcacgaag
     3841 acgtcgttgc agacgaccgt gccggtgtca ccagcccgaa cgccgagctt gccgtgcagc
     3901 ttggtcgtcg tcagccccgg tgattcaccg ggaatgacaa acgcggtgat gccgcgatgc
     3961 cgtttggccg gatcgacggt cgcgaagagc aggaacaggt tggcgacgtt ggcggagctg
     4021 atccacgact tctcgccgtt gagcaggtag ccgccctcga cttcccgcgc ccgcatccgc
     4081 agcgcgccga catccgagcc ggcgcccggc tcggtcagcc cgaacgtgcc gatccgttcg
     4141 ccacgagcca gcgccggcaa ccactgctcg cgctgggcct cgctccccca ctggaggatg
     4201 cacatgctcg ccagcccgag atgaacggtc agcgagacct gatgggacgt gtcgatccgg
     4261 cccagttc
//
LOCUS       DASUGJ010000337         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1439849, whole genome shotgun sequence.
ACCESSION   DASUGJ010000337 DASUGJ010000000
VERSION     DASUGJ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439849"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFV93_07490"
     CDS             complement(<1..619)
                     /locus_tag="VFV93_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5165220.1"
                     /translation="MTGTIQEFSNGLADAVERAGGYTVRVNARRRYGSSGIVWADGIV
                     LTADHTIERDEDISITLPDGSDLPATIAGRDPGSDLAVLRVTGLTLAAAPRAEERRVG
                     SLVLAIGRLHSESGLGASLGIISHAGTPMRGRRRRRGGGMAVESVIRPDLTMYPGFSG
                     GPLIDTSGAMVGINTSGFRGQPLAIPHTAAADVVEQLLAHGRLRRG"
     gene            843..986
                     /locus_tag="VFV93_07495"
     CDS             843..986
                     /locus_tag="VFV93_07495"
                     /inference="COORDINATES: protein motif:HMM:NF039193.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX5165221.1"
                     /translation="MEGYCVKCREKREIKDAQRVEFDNGRKAMQGVCPVCGTKIVRFV
                     KDA"
     gene            complement(1060..1191)
                     /locus_tag="VFV93_07500"
     CDS             complement(1060..1191)
                     /locus_tag="VFV93_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165222.1"
                     /translation="MRNAAICAAIAITLVGAIVVTGFQENVVLLWAAITTIVATSVR"
     gene            1547..>2314
                     /locus_tag="VFV93_07505"
     CDS             1547..>2314
                     /locus_tag="VFV93_07505"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX5165223.1"
                     /translation="MARFREKAQLDPSQVQDQRGASGGRGMAIGGVGGGLGLIILIIT
                     LLLGGNPLDGSTTQSPGATNGGNQSAQDSNLTEECQTGADANQSQDCRIVGFVNSIQD
                     FWTDEYARNNAQYVQASTVLFTDGYNTGCGYATSQVGPFYCPADQHVYLDLGFFDQLQ
                     TDFGAQGGPFAEAYVLAHEYGHHIQNLQGILSRAQSNQTGPNSPGVRVELQADCFAGL
                     WAAHAADTGFLEPLTDEDIKIALDAASAVGDDRIQQTM"
BASE COUNT          412 a          772 c          747 g          383 t
ORIGIN      
        1 agccgcgtcg caggcggccg tgggcgagca gttgctcgac cacgtcggcg gcggcagtgt
       61 gggggatcgc cagtggctgg ccccggaagc cggacgtgtt gatgccgacc atcgcgccgc
      121 tcgtgtcgat cagcgggccg cccgagaaac cggggtacat cgtcaggtcg ggccggatga
      181 ccgactcaac cgccatcccg ccgccgcgcc gtcgccgtcg cccgcgcatc ggcgtgccgg
      241 cgtgggagat gatccccagt gacgcgccaa ggccgctctc gctgtgcagg cgcccaatcg
      301 ccaggacaag gctgccgacg cgtcgctctt ccgcccgtgg cgctgccgcg agggtcagcc
      361 ccgtgacgcg caggacggcc agatcgctgc cgggatcgcg gcccgcgatc gtcgccggca
      421 ggtcgctgcc gtccggaagg gtaatgctga tgtcctcgtc ccgctcgatc gtgtgatcgg
      481 cggtcaggac gatgccgtcc gcccagacga tgccgctgga gccgtagcgc ctgcgtgcgt
      541 tgacgcgaac cgtgtagccg ccggcgcgtt cgaccgcgtc ggcaaggccg ttgctgaact
      601 cttgaatcgt acctgtcatc gtatctcttt ctcccgcatg ttcgggtcgc tcttgcatgt
      661 cggcgggacg ttcccaaccg ctaccctatc atcgtcgatg gcgcgccggc aggcatccgc
      721 gcttctggta gcaggcgaac tgtccgaatg ggtagggctg gcagcggcac catgcttgcg
      781 caggtggtcg cggcgacatg ccacgaaccg gcgcccggcc ggccatgaaa ggatggatat
      841 caatggaagg ctactgcgtc aagtgtcgcg agaagcgcga gatcaaggac gcccagcggg
      901 tcgagttcga caacggccgc aaagcgatgc agggcgtctg cccagtgtgc ggcaccaaga
      961 tcgtccgctt cgtcaaggac gcgtagagat tcgggattcg gggttgaaga tcggctctga
     1021 cccgtgaggg ctatccaccg aatcccaaat cccgaatccc taccgaaccg acgtcgcgac
     1081 gatcgttgtg atggcggccc agagcagaac gacgttttcc tggaagccgg tcacgacgat
     1141 ggctcccacc agcgtaatgg cgattgctgc gcagatggcg gcgttgcgca tcccgcgacc
     1201 ctcccggttc ctgttccggc gcgaccgcat cgcggcgccg tcccaccccg catcatcgcg
     1261 gactggccga caacgttgtc ggccgatggt tgcaccagca tatccgggcg ctggcggagg
     1321 cgatattgcc tgctggaacg gaattgcgac tgtccggttg gacatcgcca cgctgcctgg
     1381 ttgttcacat gtaacagacc tcccagaaac cccgttagaa tacgcagcgt gattcaggat
     1441 acgttcgacg cgtcggggca taggtcatgc ttttgacgtc cctgcacgcc gccggcgtct
     1501 agcccggcgt ctgttagata tgacgcaacg aaggaactga gacgagatgg cacgcttcag
     1561 ggagaaggca caactcgatc catcccaggt gcaggaccag cggggagcca gcggcggacg
     1621 tggaatggcg atcggcggtg tcggtggtgg gctcggactc atcattctga tcatcacgct
     1681 cctgctgggc ggaaacccgc tcgacggcag caccacccag tcaccgggcg cgaccaacgg
     1741 cggcaaccaa tcggcgcagg acagcaacct gaccgaggag tgccagaccg gcgcggacgc
     1801 gaaccagtcg caggattgcc ggatcgtcgg cttcgtcaac agtatccagg atttctggac
     1861 ggacgagtac gcgcgcaaca acgcgcagta tgtccaggca tcgaccgtcc tcttcaccga
     1921 cggctataac accggctgcg gctacgccac gtcccaggtc ggcccgttct actgtccggc
     1981 cgatcagcac gtctacctgg atctcggctt cttcgaccag ctacagaccg acttcggagc
     2041 gcagggcggt ccgttcgccg aggcgtatgt tctcgcccac gagtacggcc atcacatcca
     2101 gaacctgcag ggcatcctgt cccgcgcgca gagcaaccag accggtccaa acagccccgg
     2161 cgtccgggtc gagctacagg ccgactgctt cgccggcctc tgggctgccc atgctgccga
     2221 caccggcttc ctggaaccgc tgaccgacga ggacatcaag atcgcgctcg acgccgcctc
     2281 ggccgtcgga gacgaccgca tccagcagac catg
//
LOCUS       DASUGJ010000338         8187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1581191, whole genome shotgun sequence.
ACCESSION   DASUGJ010000338 DASUGJ010000000
VERSION     DASUGJ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8187
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581191"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1398
                     /gene="trpS"
                     /locus_tag="VFV93_07510"
     CDS             367..1398
                     /gene="trpS"
                     /locus_tag="VFV93_07510"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5165224.1"
                     /translation="MVATETRPQTITSTASKRRVFSGIQPSGSTTLGNYLGAIKRWAI
                     QQHQHQSIHSIVNLHAMTVPYDAADLRQRTLDMAAVLIACGIDPEVALLFAQGDVPEH
                     TELTWTMATQSMFGELNRMTQWKDKGRGDERSGAGLFFYPVLMAADILLYDTNTVPVG
                     EDQRQHVELTRDVAMRFNAHFGETFVIPEADIETEGARIMSLDDPTKKMSKSGAPGSF
                     ISFQDSPDDIRRKINRAVTDSGSEIVARADKPAMTNLLTIYSLMTDTPISELERRYDG
                     SGYGTFKREMGDALVEKMAPIQQRLAELSDDPGELMRILARGRDRAREFATPTMERVR
                     IATGIRLNV"
     gene            complement(1534..2103)
                     /locus_tag="VFV93_07515"
     CDS             complement(1534..2103)
                     /locus_tag="VFV93_07515"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5165225.1"
                     /translation="MAKLPDISDAIRFDENLREVPRWPDRIAYALRRARKQLAEAREA
                     GYDTQILRTLGYLTDASRVAGDLDAAVTYGQEALERARASGNSNAIVANLIRLGEAHT
                     YRDEPAVAEPLLREALALSSQGDSGPLQDYALQHLGKLLLETGSYDESITYLEQALAL
                     RQAKGNQPLIESTQQALDLVRARRETRDA"
     gene            complement(2114..2563)
                     /locus_tag="VFV93_07520"
     CDS             complement(2114..2563)
                     /locus_tag="VFV93_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165226.1"
                     /translation="MATLEQLAFAEHLRAVAQWRRDKMQEFDPDPRNLRSAAGLEELA
                     DYWLVLDERDDRVLEFTSLTFLGQMFRPGQRVEWEIPRFRFYYEESTVEGFLDHLLDL
                     ARQDAAETDPIGLPMVPGDNPWEHRPTRIYIVEPDDDGSRSDDTLER"
     gene            complement(2681..5005)
                     /locus_tag="VFV93_07525"
     CDS             complement(2681..5005)
                     /locus_tag="VFV93_07525"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5165227.1"
                     /translation="MAQPGPEAFAAALPDVTSTLTLAGLDQPVEIIRDHYGIPHIKAA
                     TLHDAFFAQGFVHAQDRLWQMEFDRRRAEGRWAECAGAPAIEQDRFMRRARIVDSAKA
                     DYDAFNDETKAMCDAYTAGVNAFIDTTKTLPVEYSLLDLQPRQWEPWISGGIFKVRHI
                     LMGLWGQKLWRARILKEHGPEGVAKLGVRGNEDGIVIVPPGEEYQAALADLDELIPGV
                     AALTETPEFGGSNNWAVHGSRTRSGKPLVAGDPHRFLDVPSVYYQCQITCPDFDVVGY
                     NFIGIPGFPHFAHNAHVAWCITHASADYQDLYVERFRPAGDCYQYEFQGEWQTATCAN
                     ETIQVRGGQPVAVQAVVTGHGPVIIGDPASGHAIALRYSAMDRPNPGMTCFLPQLRAA
                     NLDELDEAMRNWVDPCNSMTMADVHGNIGYLHRGRVPVRNRANGWLPVPGWTGEHEWQ
                     GDIPFEELPRLRNPETGFVATANNRIVGPEYPHYLSLDYAPPHRARRVVARLSDLRDA
                     TIADMSAIHNDRISLPSRDFVAALGTLAPDDERTAAALDRLKSWDGDMDKSKVAPAIY
                     AATRDQLARLVATRPYLSVLRENPFAGDPAPMGVEARLWASMMGMIQDNDTTLLADGE
                     TWEQLLGEALGNAVALLTEQLGENMDGWQWGKLHTTNPIHTLSGVFPELASTLNPPAV
                     SIGGDGDTVQAAGTFPGQNFHVNGTSVTRYAFDLGDWDSSAWVVPLGASGHPGSPHFA
                     DQAVDWSEVRLNPMLYSWDKVAANAETTQRLQPA"
     gene            5216..6706
                     /locus_tag="VFV93_07530"
     CDS             5216..6706
                     /locus_tag="VFV93_07530"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HEX5165228.1"
                     /translation="MRGLRLLLGLTLAGILLAGCAGGGDDNDLTATATSDASPAGTVV
                     TGLTATTPASPTTEPTRPASPTARTASPTSTPRPTVTAPIRPIATKAPTPTVTTSSDS
                     AEPVTLELVQQGFSEVQSYDEVAYGFIVQNPDSVNAVINSEYHVTVYQGDTELESDSG
                     YLDYVLPGQRIGVGGSVFIAAGSDADSIVVELTAGETAEPEIDPGFGVSGITYYKESL
                     FPSATGVVSTTYAKPLQDFEVFAVAYDAAGTIVGGGYTFVSFITAGGTTGVEVNVSSA
                     DEPASVELYPVVTSLTIYGISDTTSPDAVPLGIVAEGFGPSAYENELGWGFIVENPNE
                     NLAAETTAYQVTAYSADGIVLGVYSSYISLLLPGERLGFGGSFYLPAGTTASRLDFEV
                     LGRYFSETTLRADFLTTSNVTFVPDEFSPRVTGTIQNGLDRDLTDVEVYAVAYNAAGQ
                     IIGGGFTFVDQIAAEGQADADVTIAVGEEPAAVELYATVGSLSDIE"
     gene            6750..7025
                     /locus_tag="VFV93_07535"
     CDS             6750..7025
                     /locus_tag="VFV93_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HEX5165229.1"
                     /translation="MHGEYKTPGGKLVVADFDVRDDAFRDVVVSGDFFLYPEEALDAI
                     VGAIDGLPVTSGESAIARAVEAAIPDETEMLGFSPEAVAIALRRGLG"
     gene            7022..7831
                     /locus_tag="VFV93_07540"
     CDS             7022..7831
                     /locus_tag="VFV93_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEX5165230.1"
                     /translation="MTTEHEALYPAEVEARWQEYEWELIPAIPEVPPMNLALDEVLTK
                     SVGRGERRPTLRFWGWSAPCVVLGRFQSVRNEVQIDHAREDGIQLVRRISGGGAMFIE
                     PPLAITYSIHVPPAFVEGLSFPQSYAFFDSWVIEALRELGIDAWYAPLNDITSAGGKI
                     GGAAQARRFGGVLHHTTMAYDMNIPAMMRTLRIGQEKLSDKGLRSADKRVGPLRQQTN
                     LPRETIIERMSASFTRRTNAAAGAITVDERSEAEELVTSKFATEEWTYLLP"
     gene            7928..8080
                     /locus_tag="VFV93_07545"
     CDS             7928..8080
                     /locus_tag="VFV93_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165231.1"
                     /translation="MDDNATRRVEPTDDERQRNREREMMGFNLGLWGVAAVVVILIVL
                     GIWLVL"
BASE COUNT         1345 a         2539 c         2881 g         1422 t
ORIGIN      
        1 gccggtgaca tggcagttgc ggaatggacc cgggagttgt gcgctgaacg gtcgtctgcc
       61 tgatcgctga caggcttagt attccgcacc gggcactccc ccgttacagg gagtcgaggt
      121 ggccgcgatg tgtcctgtct gtgatggcag gaggcggcaa ccagggtggt accgcgggag
      181 cgcacttgcg ccctcgtccc tgttgggacg ggggtttttt gttgcccgtg ttacaccgtg
      241 tgagagtggt aaggaaggcc ggcatggttc attcacgacg acgctcgccg gcggagttca
      301 gccgcgcgag ctggcgcaga cagtatcgat cacgatgcat gcgttcatga gacaggagca
      361 aacttgatgg tagcgaccga aaccaggcca cagacaatca cgagcaccgc atcgaaacgg
      421 cgcgtcttct ccggtatcca gccgagcggg tcgacgacgc tgggcaacta cctgggggcg
      481 atcaagcgct gggcgatcca gcagcatcag caccagagca tccactcgat cgtcaacctg
      541 cacgcgatga cggttccgta cgacgccgcc gacctgcgcc agcgaacgct cgacatggcg
      601 gccgtcctga tcgcctgcgg gatcgacccg gaagtcgcgc tgctcttcgc tcagggtgac
      661 gtgccggagc ataccgagct gacctggacg atggcgacgc agtcgatgtt cggcgagctg
      721 aaccggatga cgcagtggaa ggacaagggg cgcggtgacg agcggtccgg ggccggactg
      781 ttcttctacc cagttctgat ggcagccgac atcctgctct atgacaccaa caccgtcccg
      841 gtcggcgagg atcagcgcca gcacgtcgag ttgacgcgcg atgtggcgat gcgcttcaac
      901 gcgcacttcg gcgagacgtt tgtgatcccg gaggcggaca tcgagaccga gggtgcgcgg
      961 atcatgagcc tggacgatcc gacgaagaag atgagcaagt ccggcgcgcc gggcagcttc
     1021 atctcgttcc aggacagccc ggacgacatc cggcgcaaga tcaatcgggc cgtcaccgac
     1081 tcgggcagcg agatagtcgc ccgtgccgac aagccggcca tgacgaacct gctgacgatc
     1141 tactcgttga tgacggatac gccgatctcc gagctggaac gccgctacga tggttccggc
     1201 tacgggacgt tcaagcgcga gatgggcgat gcgctcgtcg agaaaatggc gccgatccag
     1261 caacggctgg ccgaactgag cgacgacccc ggcgagctga tgcgtattct cgcccgtggc
     1321 cgcgaccgcg cccgcgaatt cgcgacgccg acgatggaac gcgtccggat cgcgaccggc
     1381 atcaggctta acgtctgatc attggcacgg ttggaaagca ggggccggtc ttgtgccggc
     1441 cctgctcgtg ctcccgaacg cgtcgggtgg ggagcagggc cggcacaagg cacggcccct
     1501 acttttggcg tggcgccgct cggctgggtc gcgtcacgcg tcccgtgtct cgcggcgggc
     1561 gcgtacgagg tcgagtgcct gttgcgttga ctcgatcaac ggctggttac ctttggcctg
     1621 gcgcagggcg agcgcctgtt ccagatacgt gatcgactcg tcgtaactgc ctgtttcaag
     1681 caggagcttg ccgaggtgct gtaatgcgta gtcctggagc gggccagaat cgccctggga
     1741 ggagagggcg agtgcctcgc ggagcagtgg ttcggcgacg gcgggttcgt cgcggtacgt
     1801 gtgggcttcg ccgaggcgga tcaggttggc gacgatggcg ttgctgttgc cggatgcgcg
     1861 cgcgcgttcc agcgcctcct gcccgtaggt gacggcggcg tcgaggtcgc cggcgacgcg
     1921 tgaggcgtcg gtgagatagc cgagagtgcg caggatctgg gtgtcgtagc cggcctcgcg
     1981 ggcctcggcc agttgcttgc gggcgcggcg aagtgcgtag gcgatccggt cgggccagcg
     2041 gggaacctcg cggagattct catcgaatcg gatcgcatcg ctgatgtcgg ggagcttggc
     2101 catgactggg aggctacctt tcgagcgtgt cgtcagatcg tgagccgtcg tcgtccggtt
     2161 cgacgatgta gatgcgggtc gggcggtgct cccacggatt gtcgccgggg accatcggca
     2221 ggccgatcgg gtcggtctcg gcggcgtcct ggcgggccag gtccagcagg tggtcgagga
     2281 agccctcgac ggtcgattcc tcgtagtaga agcggaagcg cggaatctcc cactcgacgc
     2341 gctggcccgg ccggaacatc tggccgagga acgtcaggga cgtgaactcg aggacgcggt
     2401 cgtcgcgctc gtccagcact agccagtagt ctgccagttc ttccagcccg gctgccgagc
     2461 gcaggttgcg cgggtcggga tcgaactcct gcatcttgtc ccgccgccac tgagcgaccg
     2521 cgcgcaggtg ttcggcaaac gccagttgct caagtgtcgc catgtcgggc ctccggccgg
     2581 ttataggtgg taggtgatag gtgttagggg ttggtccgtt cggatcggcg tgcaacgttg
     2641 ctacccgcgg gccaacccct aacccctaac acctatcacc tcaagcaggc tgtagtcgct
     2701 gcgtcgtctc ggcgttggct gccaccttgt cccaggagta gagcatcggg ttgaggcgta
     2761 cctcgctcca gtcgacggcc tggtcggcga agtgcgggct gccgggatgg cccgatgcgc
     2821 cgagcgggac gacccaggcc gagctgtccc agtcacccag gtcgaaggcg tagcgggtga
     2881 ccgaggtgcc gttgacgtgg aagttctggc cggggaaggt tcctgccgcc tgaacggtgt
     2941 cgccatcacc gccgatcgag acggccggcg ggttcagcgt cgacgccagt tccgggaaga
     3001 caccggacag ggtgtggatc gggttggtgg tgtgcagctt accccactgc cagccgtcca
     3061 tgttctcgcc gagctgttcg gtcagcagcg cgacggcgtt gccaagtgct tcgccgagta
     3121 gctgctccca ggtctcaccg tcagccagca gtgtcgtgtc gttgtcctgg atcatcccca
     3181 tcatcgaggc ccagaggcgc gcctcgacgc ccatcggcgc tggatcgccg gcgaacgggt
     3241 tttcgcgcag gacagacagg tacgggcgag tcgcgacgag gcgagccagc tggtcgcggg
     3301 tggcggcgta gatcgccggt gcgaccttgc tcttgtccat gtcgccgtcc cagctcttga
     3361 ggcggtcgag ggcggcagcg gtgcgctcgt cgtcgggcgc gagcgtgccg agggcagcga
     3421 cgaagtcccg gctggggagc gagatgcggt cgttgtggat cgcgctcatg tcggcgatcg
     3481 ttgcgtcgcg gaggtcgctg agtcgggcga cgacgcgcct ggcccggtgg ggcggagcgt
     3541 agtcgagcga gaggtagtgc gggtactccg gcccgacgat ccggttgttg gcggttgcga
     3601 cgaagccggt ctcggggttg cgcaggcgag gaagctcctc gaaggggatg tcgccctgcc
     3661 actcgtgctc gccggtccag cccggcaccg gtagccagcc attggcgcgg ttgcggaccg
     3721 gcacccggcc gcgatgcagg tagccgatgt tgccgtggac atcggccatc gtcatgctgt
     3781 tgcagggatc gacccagttg cgcatcgcct catcgagctc gtcgaggttc gcggcgcgca
     3841 gttgcggcag gaagcaggtc atgccggggt tggggcgatc catcgccgag tagcgcaggg
     3901 cgatcgcgtg gccgctggcc gggtcgccga tgatgaccgg gccgtggccc gtgacgacgg
     3961 cctggacggc gaccggctgg ccgccgcgta cctggatcgt ctcgttcgcg caggtcgccg
     4021 tctgccactc gccctggaac tcatactggt agcagtcgcc ggccggccga aagcgctcga
     4081 cgtacagatc ctggtagtcg gccgaggcgt gggtgatgca ccaggcgacg tgcgcgttgt
     4141 gggcgaagtg ggggaagccg ggaatgccga tgaagttgta gccgacgacg tcgaaatccg
     4201 ggcaggtgat ctggcactgg tagtagacgc tggggacatc gaggaagcgg tgcgggtcgc
     4261 cagcgacgag cggcttgccg gagcgggtcc ggctgccgtg gacggcccag ttgttggagc
     4321 cgccgaactc gggtgtttcg gtcagggccg cgacgccggg gatcagctca tcgagatccg
     4381 ccagcgcggc ctggtactcc tcaccgggcg ggacgatgac gatgccgtcc tcgttgccac
     4441 ggacgccgag cttggcgacg ccctccgggc cgtgctcctt gaggatgcgt gctcgccaga
     4501 gcttctggcc ccagagcccc atcaggatat ggcgtacctt gaagattccg ccggagatcc
     4561 acggttccca ctggcgcggc tggagatcga gcaggctgta ctcgaccggc agcgtcttcg
     4621 tggtgtcgat gaaggcgttg acgccggctg tgtaggcgtc acacatcgcc tttgtctcgt
     4681 cgttgaacgc gtcgtagtcg gccttggccg agtcgacgat ccgggcacgc cgcatgaagc
     4741 ggtcctgctc gatagcgggc gcgccggcgc actcggccca gcggccctca gcccggcggc
     4801 ggtcgaactc catctgccag agccggtcct gggcgtggac gaacccctgc gcgaagaagg
     4861 cgtcgtgcaa ggtggctgcc ttgatatggg gtatgccata gtggtcgcgg atgatctcga
     4921 ccggctgatc gagtccggcc agcgtcaacg tactggtcac gtccggaagg gctgccgcga
     4981 aggcctcggg tccaggttgc gccacgttcg ttctcctcac ctctcctgtc tgacaggctg
     5041 actccgcgca tgataaccgc attcaactcc ccccggtttg tgatccttcg gctcacgcct
     5101 gaggatgaca agggcgacat ggtccggacg tggtgacata tactcgcgcg cgacgatcgg
     5161 cagcgggagt gatgccgatc ggcacaagtg gttgcggacg aaaagggtgt agcgaatgcg
     5221 tggactgcgt ctcctgctcg gactgacgct ggccggaatc ctgctggccg gctgtgcggg
     5281 cggcggcgac gacaacgatc tgacggcgac ggcgacgagc gatgcctcgc cggccggaac
     5341 cgtcgtcacc ggactgacgg ccacgacccc ggcctcgcca acgacggagc cgaccagacc
     5401 cgcctcgccg acggcgcgga ccgcgtcgcc gacttcgaca ccacgcccga ccgtgacggc
     5461 gccgatccga ccgattgcga ccaaggcacc gacgccgacc gttaccacca gcagcgactc
     5521 ggctgagccg gtgacgctgg agctggttca gcaggggttc agcgaggtcc agtcgtacga
     5581 cgaggtcgcc tacggcttca tcgtccagaa tccggacagc gtcaacgccg tcatcaactc
     5641 ggagtaccac gtcaccgttt accagggcga caccgagctg gaatcggact ccggatacct
     5701 cgactacgtc ctgcctggcc agcggatcgg cgtcggcggc tcggtcttca tcgcggccgg
     5761 cagcgatgcc gattcgatcg tggtcgaatt gacggccggc gaaacggccg agccggagat
     5821 cgatcccggc tttggggtca gcggcatcac gtactacaag gagagcctgt tcccgtcggc
     5881 gaccggcgtt gtcagcacga cctacgcgaa gccgttacag gacttcgagg tcttcgcggt
     5941 tgcctacgat gcggccggca cgatcgtcgg cggcggctat accttcgtct cgttcatcac
     6001 ggccggcggg acgacaggtg tcgaagtgaa cgtctcgtcg gcggacgaac cggccagcgt
     6061 cgagttgtat ccggtcgtca ccagcctgac catctacggt atctccgaca cgacctcgcc
     6121 cgatgccgtg ccgctgggga tcgtcgccga agggttcggc ccatcggcct acgagaacga
     6181 gcttggctgg ggcttcatcg tcgagaaccc gaacgagaac ctggcggctg agacgactgc
     6241 ctatcaggtg acggcctact ccgccgatgg gatcgtgctg ggggtctatt cgtcgtacat
     6301 ctcgctgctg ctgccgggcg agcggctggg cttcggaggt tcgttctacc tgccggccgg
     6361 tacaaccgcc agccggctcg atttcgaggt gctggggcgc tacttcagcg aaacgacgct
     6421 gcgggccgac ttcctgacga ccagcaacgt caccttcgtc ccggacgagt tcagcccgcg
     6481 cgtgactggt accatccaga acggcctcga ccgcgatctg accgatgtcg aggtctacgc
     6541 ggtcgcctac aacgcggccg gccagatcat tggcggcggc ttcaccttcg tcgaccagat
     6601 cgccgctgag ggccaggccg acgccgatgt cacaatcgcc gtgggcgaag aacctgccgc
     6661 cgtcgagctg tacgcgacgg tcggcagcct gtcggatatc gagtaggcgg accgcattcc
     6721 cagcctgcga accagaggag tgattggtca tgcacggtga gtacaagaca cccggcggga
     6781 agctggtcgt agccgatttt gacgtgcgcg atgatgcgtt ccgtgacgtg gtcgtctcgg
     6841 gagacttctt cctctatccg gaggaggcgc tggacgcgat tgtcggcgcc atcgacgggc
     6901 tgccggtgac gagcggcgag agcgcaatcg cgcgggcggt cgaggcggcc atcccggatg
     6961 agacggagat gctcggcttc tcgccggagg ccgtcgcgat cgcgctgcga agggggctgg
     7021 gatgacgacg gagcacgagg cgctctatcc ggccgaggtc gaagcgcgct ggcaggagta
     7081 cgagtgggag ctgatcccgg ccatccccga agtgccaccg atgaacctgg cgctggatga
     7141 ggtgctgacg aaatcggttg ggcggggcga gcgccggccg acgctgcgct tctggggctg
     7201 gagcgcgcca tgtgtcgtgc tgggccgctt tcagtcggtc cgcaacgagg tgcagatcga
     7261 ccacgcccgc gaagacggta tccagctcgt ccgccggatc agcggcggcg gagcgatgtt
     7321 catcgagccg ccgctggcga tcacctactc gatccacgtc ccgccggcct tcgtcgaggg
     7381 gttgtcgttt ccacagtcgt acgcattctt cgactcgtgg gtgatcgagg cgttgcgcga
     7441 gctagggatc gatgcctggt atgcgccact caacgacatc acctcggccg gcggcaagat
     7501 cggcggcgcg gcccaggcgc ggcgcttcgg cggcgtcctg caccacacga cgatggccta
     7561 cgacatgaac atcccggcca tgatgcggac gctgcggatc ggccaggaga aactgtccga
     7621 caaggggctg cgtagcgccg ataagcgggt cggcccgcta cggcagcaga ccaacctgcc
     7681 gcgcgagacg atcattgagc gcatgtcggc ctcgttcacg cgccgcacga acgcggcggc
     7741 cggcgcgatc accgtcgatg aacggtccga agcggaggag ctggtcacgt ccaagttcgc
     7801 aaccgaggaa tggacctacc tgctgccgta agtgccgcgt gggctagctt gtcgtggcac
     7861 attccctgcg cctgtcctcg caacgatccc gtcgctttcg cggccgggcg attgaggaga
     7921 ggacaccatg gacgacaacg ctacccgacg agttgagccg accgacgacg aacgccagcg
     7981 caaccgcgag cgggagatga tgggcttcaa cctcggcctc tggggagtgg cggccgttgt
     8041 cgtgatactg atcgttctcg gcatctggct ggtgttgtag cacgcgctcg gtttcgatca
     8101 cattgaaagg ttccccctgt cgtcctgagc cgcagcgaag gacctccccg cttcgacgcc
     8161 gcacgcggtc aagtcaagag aggtcct
//
LOCUS       DASUGJ010000339         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1025630, whole genome shotgun sequence.
ACCESSION   DASUGJ010000339 DASUGJ010000000
VERSION     DASUGJ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025630"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFV93_07550"
     CDS             complement(<1..287)
                     /locus_tag="VFV93_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165232.1"
                     /translation="MRLMSPTGHLGFTPIERGSFEIGLRQKPDAIVADSGSCDIGPYP
                     LGSDQAHSPREWQLHDIELIVRGARELGIPAIIGSASDTGTDRGVNDYVDMV"
     gene            593..1276
                     /locus_tag="VFV93_07555"
     CDS             593..1276
                     /locus_tag="VFV93_07555"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX5165233.1"
                     /translation="MADRLDLTFRPATAPRSGPDDDAEPLSSGEMYHLMNAAIAPRPV
                     AWVSTVSVDGVLNLAPHSYTTIFATDPPVVGFVSVGRKDSLNNAEATREFVLNVAGQS
                     LLNQLNLTAANFPPDEDEFQWTGLTPVASDIVTPPRVGEAPVSFETTVHDIIPVGNCH
                     LILGEVARVHVARDVWHDGRIDPQLLDPVLRLAGSMFAELGETFNLRRPTYQDVLDGK
                     LDRREKGTP"
     gene            1273..2253
                     /locus_tag="VFV93_07560"
     CDS             1273..2253
                     /locus_tag="VFV93_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5165234.1"
                     /translation="MSAQPLQPLAGYHHMTMVTKDAPTNARFYRDTLRLRRVKKTVNF
                     DMPYTSHLYYGDTTGAPGTLLTFFEWPNAEPGRRGWGGTHHIALSVASGAVLAWWRGH
                     LEREGVEVQGPFDDHGYPTIRFRDPDGLLLELSAPAGEDVPDGAPALWAEGLAIGALH
                     HVNIQATNRETAIIYYRDLLGFELTGEGTNPDHPLLTDLLFTLAGKVEEPTMRLIVTL
                     VDREQTEHAAEGPGQTHHIAFAALDDAVELDWQERLASAGIPVSEVRDRNYFHSIYFN
                     DPDGHLLEIATNNPGFAVDEPLESLGEALMLPAQYEERREQLERTLTRID"
     gene            complement(2304..>2984)
                     /locus_tag="VFV93_07565"
     CDS             complement(2304..>2984)
                     /locus_tag="VFV93_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5165235.1"
                     /translation="GDSATFQMEDMRNTRLSVVGVFSNSLGTADMLMARDDLLPHLRE
                     PLSNLLLVRLADGTDPQTIALQIDALSGQGYPTQVLTHDEYVAGVEDASTSDDWAIFL
                     IIGGAALFGALGVVNTLTMSTLDRSREFALLRLIGATPQQVMRMLAQEALLISALGVT
                     LGWGIGLLSTLSVSFGFVGDASAFTVPLPPVLAVGALATLLVFVATLLPGALALRRAP
                     VTEIGARE"
BASE COUNT          535 a          991 c          956 g          502 t
ORIGIN      
        1 accatgtcaa cgtagtcatt gacaccccgg tcggtccccg tgtcggaggc agagccgatg
       61 atggccggga tgccgagttc gcgtgccccg cgaacgatca gttcgatgtc gtgcaactgc
      121 cactcgcgcg ggctgtgtgc ctgatcggag ccaagcgggt acgggccgat gtcacagctg
      181 cctgagtcgg cgacgatcgc gtccggcttc tgccgcagac cgatctcgaa actcccgcgc
      241 tcgatcggcg taaagccgag atgcccggtt ggcgacatta ggcgcatttg ccgttgcatg
      301 ctcatcagcc tcaaatctca ccgttctcgc acggagggaa cacgaccagg atcgtcctga
      361 tcttaccctc gttgccgacc aacccatagg ctggtacaga cgtgtctcac agcctcaatc
      421 gcaacgatca tagcacctta cgtcatatac tgcaatgatg attgcgtaaa cgaggctcgc
      481 atcatgcttt tgcatatgtg gcgcgacgcg ttgcgccccc tacggagacg cgcgacgcga
      541 tacccttagc gactaatatc gtcacgacgc tcatgaaacg ggagcatcac cgttggccga
      601 tcgccttgac ctgacatttc ggcccgccac tgccccgcgc tccggacccg atgacgacgc
      661 cgaaccgctc tcgtccggcg agatgtacca cctgatgaac gcggcgatcg cgccacggcc
      721 ggtcgcctgg gtgtcgacgg tctcggttga tggcgtcctg aacctggcgc cccactcgta
      781 cacgacgatc ttcgcgaccg atccgccggt ggttggcttc gtctcggtcg gccgcaaaga
      841 ctcgctcaac aacgccgagg cgacgcgcga gttcgtgctg aacgtcgccg gccagtcgtt
      901 gctgaatcag ctcaatctga cagccgccaa cttcccgcct gatgaagacg agtttcagtg
      961 gacggggctg acgccggtcg cgtcggacat cgtcacgcca ccgagggttg gtgaggcgcc
     1021 ggtctcgttc gagacgaccg ttcatgacat cattccggtt ggcaactgcc acctgatcct
     1081 cggcgaggtc gcccgcgtcc acgtcgcgcg ggatgtgtgg catgacgggc ggatcgaccc
     1141 gcagttgctc gacccggtcc tgcggctggc cggctcgatg ttcgccgagc tgggcgagac
     1201 gttcaacctg cgccgcccga cgtatcagga cgtgctggat ggcaagctcg accgccgaga
     1261 gaagggaacc ccatgagcgc gcaacccctg caaccgttgg ccggctacca ccacatgacg
     1321 atggtgacga aggacgcgcc caccaacgcc cgcttctatc gcgacacgct gcggctgcgg
     1381 cgggtgaaga agaccgtcaa cttcgacatg ccgtatacgt cccacctcta ctacggtgac
     1441 acgaccggcg cgccgggcac gctgctgacc ttcttcgagt ggccgaacgc agagccgggc
     1501 cggcggggtt ggggcgggac gcatcacatc gcgctctcgg tcgccagcgg cgcggtcctg
     1561 gcctggtggc gcggtcatct ggagcgtgag ggggtcgagg tccaggggcc gttcgacgac
     1621 cacggctatc cgacgatccg cttccgcgac cccgacggcc tgctgctgga gctgtcggcg
     1681 ccggctggtg aggatgtgcc ggacggggcg ccggcgctct gggccgaggg gctggcgatc
     1741 ggcgcgctcc accacgtcaa catccaggcg accaaccgtg agacggccat catctactac
     1801 cgcgatctgc tcggcttcga gctgaccggc gagggcacca accccgatca tccgctgctg
     1861 accgacctgc tcttcacgct ggccggcaag gttgaggaac cgacgatgcg gctgatcgtg
     1921 acgctggtcg atcgcgagca gaccgagcac gccgccgaag gccccggcca gacccaccac
     1981 atcgccttcg cggccctcga tgacgccgtt gagctggact ggcaggagcg gctggccagc
     2041 gccggtatcc ccgtctccga ggtacgcgac cgcaactact tccactcgat ctacttcaac
     2101 gaccccgacg gccacctgtt ggagatcgcc accaacaacc ccggcttcgc cgtcgatgaa
     2161 cccctcgaat cgctcggcga agccctcatg ctgccagcgc agtacgaaga acgtcgcgag
     2221 cagttggagc ggacgctgac gagaatcgat tgaattggga tgcctcgatc agaggcacct
     2281 ggcatggact ccggcgctga agctcattcg cgtgcgccaa tctcggtcac cggtgcgcgc
     2341 cgcagagcga gggcgccggg gagcagggtg gccacaaaga cgagcagcgt ggcgagtgcg
     2401 ccgaccgcca gcaccggggg cagtggcacg gtgaacgcgg acgcgtcgcc gacgaagccg
     2461 aaactgactg acagcgtgct gagcagtccg atgccccagc ccagtgtgac tcccagtgca
     2521 ctgatcaaca gggcttcctg tgccagcatg cgcatcacct gctgcggcgt tgcgccgatc
     2581 aaccgcagca gcgcgaactc gcggctgcgg tcgagagtag acatcgtgag ggtgttcacg
     2641 acgcccagtg cgccgaagag cgccgcgccg ccgatgataa ggaagatggc ccagtcgtcg
     2701 ctggtgctgg cgtcttcgac tccggcgacg tactcatcgt gggtcagcac ctgcgtcggg
     2761 taaccctgcc cactcagggc atcgatctgt aaggcgatgg tctgtgggtc cgtgccatcg
     2821 gcgagtcgga cgagcagcag gttgctgagt ggctcgcgca ggtggggcag caggtcgtca
     2881 cgcgccatca gcatgtcggc ggtgccaagc gagttggaga agacgccaac cacgctcagc
     2941 cgcgtgttgc gcatgtcctc catctgaaag gtggcgctgt cgcc
//
LOCUS       DASUGJ010000340         2334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_746955, whole genome shotgun sequence.
ACCESSION   DASUGJ010000340 DASUGJ010000000
VERSION     DASUGJ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746955"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..715)
                     /locus_tag="VFV93_07570"
     CDS             complement(248..715)
                     /locus_tag="VFV93_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5165236.1"
                     /translation="MIDQDDIDQLLDNLREYPDRLRDLTAGVDDDELSRAAAGGGWGP
                     VEIFCHLRDIEELFVERVGRILNEDDPFLAVVDESLWPIERDYAAQNARVALEQFAEN
                     RSQFTRLLSRLDAPDWHRRGHHAELGEQTVLWYARHAAEHDVVHEQQLRDLLA"
     gene            complement(712..2205)
                     /locus_tag="VFV93_07575"
     CDS             complement(712..2205)
                     /locus_tag="VFV93_07575"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX5165237.1"
                     /translation="MQSARRFFSRTPIEQATDTAGAEPAPPATVARPKVLRIEARLYQ
                     EMIEHLREVHPHEGCGLIAFSDGEPVKIYPGTNILRSSSRYRMSDLEVLRAVDDMDRQ
                     GWWLGAIYHSHPNSQPVPSATDLREANWPEALMLIASRLTSETPDLRAYRMLDNGLSY
                     EEVSIDVYVERIPWLATVRRNGFGRSARAAGQRRPRRVVWKPVGFDGTVAVARGSEGS
                     TMPELAIDDTLDFDEDGEDRRAVIGILGGMGPLATADLYQKIIEFTPAASDQEHIPVV
                     IYADPRVPDRTEALLRDGEDPTPWLVHGARTLTGMGADFIVMPCNTAHAFLDGVQPAV
                     DKPIMSMIDAAADEIRKDFPEATTVGLLATSGTITSEIYQRALQARGVDVIVPDDDLQ
                     RRCVMAAIREVKAGRTGPAATALLAEAARQLSERGADVLLAACTEIPVIFQQQHTETP
                     LVDATGALARIAVETARHLDELAKTGSPQWETSTSGWGIEPGRLAGR"
BASE COUNT          364 a          766 c          794 g          410 t
ORIGIN      
        1 ccagattaca gcccgcgcat catcacatcc tcatggaccc gttcgccgat agagcccgcc
       61 gacaccccac catgaccgcg accatgtcgc gcatacatca tctgcatcat tggcacgaac
      121 attgtatgtg tgacggctgc atctgtaaag agccagattg tcacagttcg gaagcgtggc
      181 ccaaattatt gtctatgtcg aactgcattg aacctacgtt caccacgtcg gaggatcggc
      241 actggctcta cgccagcagg tcccgcaact gctgttcgtg gacgacgtcg tgctcggcgg
      301 cgtggcgggc gtaccagagg acggtctgtt cgccgagctc ggcgtggtgg ccgcggcggt
      361 gccagtcggg tgcgtcgagg cgcgagagca gccgcgtgaa ctgggagcgg ttctcggcga
      421 actgttcgag ggcgacccgc gcgttctggg cggcgtagtc gcgctcgatc ggccagagcg
      481 actcatcgac gaccgccagg aacggatcgt cctcgttcag gatccggccg acgcgctcga
      541 cgaatagctc ctcgatatcg cgcaggtggc agaagatctc gaccggcccc cagccgccgc
      601 cggccgctgc ccgcgagagc tcgtcgtcgt ccacgccggc cgtgaggtcg cggaggcggt
      661 cggggtattc gcgcaggttg tcgagcaact ggtcgatgtc gtcctggtcg atcatcggcc
      721 ggccagccgc cccggctcga ttccccagcc gctggtcgag gtctcccact gcggcgagcc
      781 ggtcttggcc agttcgtcga ggtggcgcgc ggtctcgacg gcgatgcggg ccagcgcgcc
      841 ggtggcgtcg accagcggcg tctcggtgtg ttgctgctgg aagatgaccg gaatctcggt
      901 gcaggccgcc agcaggacat cggcgccccg ctcgctgagt tgccgggcgg cttcggccag
      961 cagcgccgtc gcggcaggac cggtccggcc ggccttgacc tcgcggatcg cggccatcac
     1021 gcagcgccgc tgaaggtcgt cgtcggggac gatcacatcg acgccacggg cctggagcgc
     1081 acgctggtag atctcgctgg taatcgtgcc gctggtcgcc agcaggccga ctgtcgttgc
     1141 ctccggaaag tccttgcgga tctcatcggc cgctgcgtcg atcatgctca tgatcggctt
     1201 atcaacggcc ggctggacac cgtcgaggaa ggcatgggcc gtgttgcagg gcatgacgat
     1261 gaagtcggcg cccatccccg tcagcgttcg cgcgccgtgg accagccagg gcgtcgggtc
     1321 ctcgccatcg cgcagcagcg cctcggtccg gtccggcacg cgtgggtccg cgtagatgac
     1381 gaccgggata tgctcctggt cgctggcggc tggcgtgaat tcgatgatct tctggtagag
     1441 gtcagctgtt gccagcggcc ccatcccacc gaggatgccg atgacggccc gccgatcctc
     1501 gccgtcctcg tcgaagtcca gtgtgtcgtc gatagccagc tccggcatcg tcgacccctc
     1561 cgagccgcgc gcgacggcga cggtgccgtc gaacccaacc ggcttccaga ccacccggcg
     1621 cgggcggcgc tgtcccgccg cacgagcgct gcgtccgaaa ccgttgcggc gaacggtagc
     1681 cagccacggt atgcgctcga catagacgtc gatcgagact tcttcgtagg acaggccgtt
     1741 gtcgagcatg cggtaggcgc gcagatcggg cgtctcggac gtcagccgtg aggcgatcag
     1801 catcaacgcc tcgggccagt tggcctcgcg caggtcggtc gccgacggta ccggctggga
     1861 gttggggtgc gagtggtaga tcgcgccgag ccaccagccc tggcggtcca tgtcatcgac
     1921 ggcccgcagg acctcgagat cggacatccg gtagcgtgag gaactgcgca ggatgttcgt
     1981 gccggggtag attttgaccg gctcaccatc ggagaaggcg atcagcccac agccttcgtg
     2041 cgggtggacc tcgcgcaggt gctcgatcat ctcctgatag agccgcgctt cgatccgcag
     2101 gaccttcgga cgcgccaccg tcgcgggcgg tgccggttcg gcgcccgctg tgtcagtcgc
     2161 ttgctcgatt ggggttcggc tgaagaagcg acgcgcggat tgcatggccg tccttgctcc
     2221 cgcggggtgt ctgcgacccg acaatgtctg cacgcgctga tctggtgacc gactccgaag
     2281 gaaacaggct ccgccaccag cacgaggttt tgcccgcgca gtctcgaaag tata
//
LOCUS       DASUGJ010000341        11460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_330691, whole genome shotgun sequence.
ACCESSION   DASUGJ010000341 DASUGJ010000000
VERSION     DASUGJ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11460
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330691"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..4493)
                     /locus_tag="VFV93_07580"
     CDS             complement(99..4493)
                     /locus_tag="VFV93_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX5165238.1"
                     /translation="MIERVRRANPYHLGLLLIVAVGLFLRAYGLNWDEGLYLHPDERF
                     IAIVSSSRVDMPPAGDLSSLFDPETSPINPRRIDPATGAPMSFAYGTLPVYVQSVTAW
                     AVNLVTDTDYQSYPHIYKVGRVLNVLLDTLTIVLVFLLAKRLFRPSAGLVAAALYALA
                     VLPIQLSHFFTVDIWLTFFVTAALYVAIRYVDQPSFGRALALAVPVGCAFATKASVPS
                     LIVPLLVMAGWALWRTDDRLMVVSSLLSAGALSILVFSVFEPYAIINSGPFIEDIRVQ
                     SRIVRGEFDVPFTRQFVGLTPGLYELRNAFLYTLGPGLLLAGLAGVVYAARRCWKRFD
                     PALLIALSWIVAYLPTLLLIEARFLRYTLPLVPVLAALAGGLLTTRVRVERRALLQLA
                     TTAVLIVTAVWAIGFTTIYSRENSRIAASKWMAANVPAGSAVASETWDDVLPLPYAGS
                     SLMGYQYVEFKIYDDAPPDEKAQYIAETLMRSDYVVLSSDRVIESVDNLPWRYAVQNE
                     YYRRLLAGQLGYQLVYQSDLRPELFGYRFDDSAADESFTVYDHPRVRIFKKVENLDAG
                     QIRDRLLWGINQPWEPQRYPAEKQLMLGEPVSEIETTNDAGWNTLAMDHALIALVVWL
                     AAVELIGLAVLPLAAGILGRTPDRGAFSARLLGLILLGWVGWIAATLDFWQSRSLNIA
                     LALAAIAAACWGYWRWREKRGSPIAMPGRRAYLASLAAWLGLFSFFLLLRAIYPDFWQ
                     TWFGGEKPFELAYLRAVSASTSFPPYDPWYADGIINYYYYGWHLIGTIIKLSGVGVSY
                     GFQFGSATFAALLGLQSASLGAMLFRRGRPRIPARAVAAGGAVAVMAVLFIGNLDAFR
                     QVIEQRGVQGDTFDFWRSRSVIDFTINEFPYFSEIWADLHPHVLNFPSILLLLTLLAQ
                     IVLGGLSTTWRGLLPVAAVTSLVLGTVAITNSWDAPLMVLITGAAFVYIGLLRSPQAA
                     LTTGVLGALVVAGAFLIYTPFYLGFYSVVQGLNRASAGSDLGQFLTHWGIFFGVVVVV
                     CARDLVRRLPSDLVLRDGLLLAVITLIGGTFAELLAIAMGDGLPLPQAVIAILLAATV
                     VGLAAGATRCIQQPALFVGIVAALAFLSGTVALYRPSAAVALAITTAATMHLVSYRRQ
                     PSRFLPWALVAIGAVTTASTEFVYVADDLQHSDWERMNTVFKFYLQSWILLAIGCAVL
                     VGWLWREARLSGPPYLTQAAGIIAGTARRASVAGAARDLSTRSARVAAIATSVLLALG
                     MIYPVVGTPVRLDWDMDSSPSGLSLDGYAWMNGGLILNGTNEPIQFTGDLAAIDWLNA
                     HVDGTPVILEAAIGPYRGNGSRISSATGLPTVLGWDRHQRQQRYEPGISERMADVHDI
                     YNETNSALKLEELRRYRVRYVIVGDVERYWNVPENPTHYASTEGLAAFEQLVGNGLEI
                     AFESGSTRIYEVTDFPRIAPAPGAVVEL"
     gene            4625..5134
                     /locus_tag="VFV93_07585"
     CDS             4625..5134
                     /locus_tag="VFV93_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HEX5165239.1"
                     /translation="MRTLKVIVGGEGNVGKTSLIRQYAKAKFSEARNITLGIDITTQE
                     FSLDGEAVKLAVWDIEGQAGNRPNFYLGAQAAMLVYDVTSDVSLQALAEWFARCKRYC
                     PTAPIVIVGNKTDLGLSFPAEWGRSFATFANAQHGFVSAKTGENVSRGFELLARMALN
                     HASEGASAV"
     gene            5131..6075
                     /gene="fmt"
                     /locus_tag="VFV93_07590"
     CDS             5131..6075
                     /gene="fmt"
                     /locus_tag="VFV93_07590"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5165240.1"
                     /translation="MRAVFLGSPAFAVPSLVALARHNNIEIPLVVTQPDRPAGRGRAL
                     TPPAVKTAALELGLPVYQPDTLRDDAAVQPLRDDSPDLLIVVAYGELLRRNVLELTPA
                     GCLNVHPSLLPRYRGAAPIPAAILNGDAQTGVSIMKLVRKLDAGPIVAQFATDIGPDE
                     TTATLSDRLAELAARELPSVALAYLAGDSAPVPQDDDAATYTREWSTADALIDWSHPA
                     DQIERLVRAANPWPVAWTTDGETRLRILTARIAATGTGQPPGTVAIVNGRVVVATGAG
                     DLHLDLVQPAGKRAMPAADWWRGTRRATAVLGTSLDRS"
     gene            complement(6206..8041)
                     /locus_tag="VFV93_07595"
     CDS             complement(6206..8041)
                     /locus_tag="VFV93_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HEX5165241.1"
                     /translation="MRSAVRGQVMIIFALASLVLVGFIALSIDTGFLMAERRQVQNAA
                     DAAALAAAVSIQDDLSSGDVTAAAVAYAAENAGVSADDVTVNWPPAAGSAYAGDSNYV
                     QVTVNKEVDRFFIGAIYTGPWEVSASAIAGLEPEGFDAAILALNSDAGGINTSGATSI
                     RAICGSIVSNYEIRTSGSTTIAAQGGSGCPSPLSAYVVANDGFNTSGSTNINGESGVK
                     PHGAEVPDPLADKIDPPTVPSVPSNPVGTVNPANGNCRTYSPWSNPVTYTITSSKYTS
                     GGSSCVNIQNIPGGQTMSLQSGSYRFDNGAGITVGGGNGGDIVLQGGTYNFNGSSGGI
                     NIGGATPYFEMKSGNYSFNGGTGINIGGSANNNIIGGGNLYFGGGSEIHTGGSNKLTI
                     GPGTYIFNGGDGLRMSGSDRLHFSSGNYTMYFVNGADLAFSGSSRITFDSGVYVKAYF
                     LGGSGSNWSDLEMSGSTSFALPSGEYIFDHGRFLNSGSSTISGDEVFLYFCDGGYLSS
                     SGSASFGFTAPDSMIYSGYYPGVFMYSDRANTATFHWTGSTSSVSRGTIYLPSSPVQT
                     AGAATGTQFEGQFIADRFITSGATGLTVRFVEYVQTQIPMIYLVD"
     gene            complement(8332..9009)
                     /locus_tag="VFV93_07600"
     CDS             complement(8332..9009)
                     /locus_tag="VFV93_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165242.1"
                     /translation="MADRTAPAGQSTPVDEPDGLTALYTTTFEDQAGQLFAVGDMDSG
                     AHAMISDGRYVVDVDDGEWQVITPGPMPHPRNGVIEADVTLSGAGYAGLVARSTVDAD
                     GNDSMYVCLMDDTGWAGCVASHGGEYRDLFFGPIEGYQAGASQRLRLTVVEDRIEFVV
                     NNRLIGATQDSTVQIGAWGVHAESQSEATTTVSFDNLTIYTVDGQSIPTASSANLHSP
                     ASDVRFA"
     gene            9436..>11460
                     /locus_tag="VFV93_07605"
     CDS             9436..>11460
                     /locus_tag="VFV93_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5165243.1"
                     /translation="MDNPVTTIDWPADVALTHLPFVGRDAELAASEEAHGRAIAGAGQ
                     LVLLSGAAGMGKTRLAEQVATIARSHNAMVAWGSCHEWEGAPAYWPWTQILRTCIRVH
                     GTEMLPQLSPVQRDLLNQIVPELQDHDRVPDRLGHLEPEAQLFQVFAATSALVRHVAD
                     HQPLVIVLDDTHWADTPTLQLLRFLAVDIREAAVLIIATYRSDELGPSDAVLSLLTDL
                     YGETHCLRLALRGLAKHHIADLVALVAAEAQPAPLVDAIFEETDGNPFFVAEVVLLLA
                     QEGSLGASAVTARRLQVPANVRAAIRQRVARLSPDCRQVLTAASVIGRDVNIRILARA
                     TQLAAPVLMDALDDASRAQLIVPGDSPRTFRFVHALVQESLYQELTGSERVRLHLAIG
                     AALEASVDVEPHWEELAHHYVQAGPFGDPAKTLQYTEQAGLVAMSHFAWQTAANHFAH
                     ALDALELSNPSDTARRCHLLLALGDAQNRSGPGSGDSPTARVSFLEAFRLAESRGDHT
                     SMAQAAIGFAGLNVVTAFGAEQQELLECSLAALPTTDSSLRVRVLSRLAVDLWNRSTA
                     SLRRASGLVDEAFAVATRMDDVGLQSFALWARHYSGWRPDNLDERIAVASRLIALSEQ
                     TGDPVVTAWGYISRTLDCVEAADLFAASQSLTILGQFVERVHIPYVALRET"
BASE COUNT         2154 a         3602 c         3766 g         1938 t
ORIGIN      
        1 cgggccgagg cgttcgccga gccagtagac gaatggaaac agcgcggcgg cgatcagtgt
       61 cagggcgatg taccagcgca gaatggcttc gaggtcgctc acagctcgac gaccgctcct
      121 ggcgccggcg cgatgcgtgg aaagtcggtc acttcgtaga tcctggtcga gccggactca
      181 aaagcgatct ccagcccgtt gcccaccagt tgttcgaagg cggctaagcc ttcggtcgat
      241 gcgtagtggg ttgggttctc cggcacgttc cagtagcgtt cgacatcccc gacgatgacg
      301 tagcgcacgc ggtagcggcg tagctcttcg agcttgaggg ccgagttcgt ctcgttgtag
      361 atgtcgtgga catcggccat ccgctcagag ataccaggct cgtagcgctg ctggcgctgg
      421 tggcggtccc agccgaggac ggttggcaac ccggtcgcgc ttgagattcg cgagccgttg
      481 ccccgatagg gaccgatggc ggcttcgagt atgaccggcg tgccgtccac gtgcgcattg
      541 agccagtcga tcgcggccag atcaccggtg aactgaatcg gctcgttggt gccgttgagg
      601 atcaggccgc cgttcatcca ggcatagcca tcgagcgaga gtcccgacgg cgacgagtcc
      661 atgtcccagt cgagccggac gggtgtgccg actacgggat agatcatccc gagcgccagc
      721 aggacactgg tcgcaatcgc ggcgacgcgg gcggaacggg ttgagagatc gcgggccgcg
      781 ccggccacgc tggctcggcg ggcggttccg gcgatgatcc cggctgcctg ggtcaggtac
      841 ggcgggccgc tcaaccgcgc ctcacgccag agccagccga ccaggactgc acaaccgatc
      901 gccagcagga tccacgactg gagatagaac ttgaagacgg tgttcatccg ctcccagtcg
      961 ctgtgctgga ggtcgtcggc gacgtagaca aactcggtcg aggccgtcgt gacagcgccg
     1021 atcgcgacca acgcccacgg caggaaacgg gaaggctggc gccggtagct gaccagatgc
     1081 atcgtggcag ccgtcgtgat cgccagcgcg acggctgccg acggccggta gagcgcgacg
     1141 gtgccggaca ggaacgcgag cgccgccacg atgccgacga acaacgccgg ctgctggatg
     1201 cagcgggttg caccggcggc caggccaaca acagtcgccg ccagcaggat cgcgatcacc
     1261 gcctgtggca agggcaggcc gtcgcccatg gcgatcgcca gcagttcggc gaaggtcccg
     1321 ccgatcagcg tgatgacggc cagcagcagg ccgtcgcgca gcacgaggtc ggagggcaag
     1381 cggcgaacca gatcgcgggc gcagacgacg acgacgaccc cgaagaagat gccccagtgc
     1441 gtcaggaact ggccgaggtc ggaaccggcc gaggcgcggt tcagtccctg gacgacgctg
     1501 tagaacccca gatagaacgg cgtgtagatc aggaacgccc cggccacgac cagcgcaccg
     1561 agcacgccgg tcgtcagcgc ggcctgcggc gagcggagca ggccgatgta gacgaaggcg
     1621 gcgccggtga tcagcaccat cagtggtgcg tcccacgagt tggtgatggc gaccgtcccg
     1681 agcacgagcg aggtgacggc ggccaccggc aacaggccgc gccaggtggt cgacagcccg
     1741 ccgaggacga tctgggccag cagcgtcagc agcaggagga tggatggaaa gttgagcaca
     1801 tgcggatgga gatccgccca gatctccgag aagtagggga actcgttgat cgtgaaatcg
     1861 atgacgctgc gggagcgcca gaagtcgaac gtgtcaccct gaactccccg ctgctcgatg
     1921 acctgccgga aagcgtccag gttgccaatg aagaggacgg ccatgacggc aacggcgccg
     1981 ccggccgcga cggccctggc cggtatgcgt ggccggcccc ggcggaagag catcgcgccg
     2041 agcgaggcag attgcagacc gagcagagcc gcgaaagtgg ccgagccaaa ctggaagccg
     2101 tacgagacgc cgacgccgct cagcttgatg atcgtcccga tcagatgcca gccgtagtag
     2161 tagtagttga tgatgccgtc ggcgtaccac gggtcgtatg gagggaacga cgtcgacgcg
     2221 ctgacggccc gcagataggc cagctcgaac ggcttctcgc cgccgaacca ggtctgccag
     2281 aagtcgggat agatcgcccg cagcagcagg aagaagctaa acagccccag ccaggcggcg
     2341 agtgaggcaa gataggcacg cctgcccggc attgcgatcg gacttccgcg cttttcgcgc
     2401 cagcgccagt agccccagca ggcggcggcg atcgcggcca gcgccagcgc gatgttcagc
     2461 gagcgcgact gccagaagtc gagcgtcgcc gcgatccagc cgacccaacc gagcaagatc
     2521 agacccagca agcgagccga gaacgcgccg cgatccggtg tccggccgag aatgccggcg
     2581 gccagcggca gcacggccag cccgatcagt tcgacggccg ccagccagac gacgagcgcg
     2641 atgagggcgt ggtccatggc gagcgtgttc cagccggcgt cattggtcgt ttcgatctcg
     2701 gagaccggct cgccgagcat cagttgcttc tcggccgggt agcgttgcgg ctcccatggc
     2761 tggttgatgc cccagagcag ccggtcgcgt atctggcctg cgtcgaggtt ctcaaccttc
     2821 ttgaagattc tgacgcgggg atggtcgtag acggtgaagc tctcgtcggc ggcggagtcg
     2881 tcgaagcgat aaccaaacag ttccggtcgc aggtcggact ggtagacgag ctggtagccg
     2941 agctgaccgg ccagcagccg tcggtagtac tcgttctgca ccgcgtaacg ccagggcagg
     3001 ttgtcgaccg attcgatgac gcggtcgctg gacaacacga cataatctga gcgcatcagc
     3061 gtctcggcga tgtactgcgc cttctcatcg ggcggcgcgt cgtcgtagat cttgaactcg
     3121 acgtactggt agcccatcag cgacgagccg gcgtagggga ggggcaggac gtcgtcccag
     3181 gtctcggagg cgacggcgct gccggccggg acgttggcgg ccatccactt cgaggcggcg
     3241 atccgcgagt tctcgcgact gtagatcgtg gtgaagccga tcgcccagac ggccgtcacg
     3301 atcaggacgg ccgtcgtcgc cagttgcagc agcgcgcgac gctcgacccg cacccgcgtc
     3361 gtcagcagcc cgccggccag tgcggccagg accggcacga gcgggagggt atagcgcagg
     3421 aagcgcgctt cgatcaacag caacgtcggc agataggcga cgatccagct cagcgcgatc
     3481 agcagcgccg ggtcgaagcg cttccagcag cgccgggctg catagacgac gccagccagc
     3541 ccggccagca gcaagcccgg cccaagcgtg tagaggaagg cgttacgcag ctcatacagg
     3601 ccgggcgtca ggccgacgaa ctggcgtgtg aatggcacat cgaactctcc acgcacgatc
     3661 cgcgactgga cgcgaatgtc ctcgatgaat ggaccgctgt tgataatcgc gtacggctcg
     3721 aagactgaga agacgagaat cgacagcgcg ccggcgctca ggagggacga gacgaccatc
     3781 aaccggtcat ccgtgcgcca gagcgcccaa ccggccatca caagcaacgg cacgatcagc
     3841 gacggaacac ttgccttggt cgcgaacgcg caaccgactg gtacagccag cgccagcgcg
     3901 cggccgaagc tgggttgatc gacatagcgg atcgcgacgt agagcgcggc cgtcacgaag
     3961 aaggtcagcc agatatcgac cgtgaagaag tgactgagtt ggatcggcaa gacggccagc
     4021 gcgtagaggg ccgccgcgac gagaccggcc gagggccgaa agagccgctt tgccagcaga
     4081 aaaacaagga cgatcgtcag tgtgtcaagc aggacgttca ggactcgccc gaccttatag
     4141 atgtgcgggt aggattggta atcagtatcg gtgacgaggt tgacggccca ggccgtgaca
     4201 gactggacgt agaccggtaa ggtgccgtag gcgaacgaca tcggcgcgcc ggtggcaggg
     4261 tcgatgcgac gagggttgat cggactcgtc tcaggatcga acaagctgga caggtcaccg
     4321 gccggcggca tatcgaccct gctcgacgag acgatcgcga tgaagcgctc atcgggatgc
     4381 aggtagaggc cctcgtccca gttgagtccg taggcacgca gaaagagacc gaccgcaacg
     4441 atcagcagca atccgaggtg gtatgggttc gcgcggcgca ctcgttcgat catgacgccg
     4501 aatcttacct gtccgggatg acgccgccac aattgaccgg cgtaccgctg ctctctatac
     4561 tccatcacga ccaggcctga ccggcgaact gtgccggcgg gcaactcgca agggagaaat
     4621 ccgtatgcgc acgctgaagg tcatcgtcgg cggagaaggt aatgtcggca agacgtccct
     4681 gatccgtcag tacgccaagg cgaagttcag tgaggcgcga aatattacgc tgggcatcga
     4741 catcacgact caggagttct cgctcgacgg cgaagcggtc aagctggccg tctgggacat
     4801 cgagggccag gccggcaacc ggccgaactt ctacctcggc gcgcaggccg cgatgctcgt
     4861 ctacgacgtg acctccgatg tctccctcca ggcgctggcc gaatggttcg cgcgctgcaa
     4921 gcgctactgc ccaacggcgc cgatcgtgat cgtcggcaac aagaccgacc tcggtctctc
     4981 gttcccggcc gagtggggcc gctcgttcgc taccttcgcc aacgcccagc acggcttcgt
     5041 ctcggccaag accggcgaga acgtctcgcg cggcttcgag ctgctggcgc ggatggccct
     5101 gaaccacgcc tcagaaggcg cgtcggctgt ttgagagccg tcttcctcgg cagcccggcc
     5161 ttcgccgttc cctcactcgt cgcgctcgcc agacacaaca acatcgagat cccgctggtc
     5221 gttacgcagc ccgaccgccc agccggtcgt ggccgcgcgc tgacgccgcc agccgtcaag
     5281 acggccgccc tggagcttgg gctgccggtc taccagcccg acacacttcg cgatgacgcg
     5341 gccgtccagc cgttgcgcga tgactcgccc gacctgctga tcgtcgtcgc ctacggggag
     5401 ttgctgcgcc gcaatgtcct cgaactgacg ccagccggct gcctcaacgt ccatccatca
     5461 ctgctgccac gctatcgtgg tgctgccccg attccagcgg cgatcctgaa cggcgacgca
     5521 cagaccggcg tcagcatcat gaagctggtg cgcaaactgg acgccgggcc gatcgtcgcc
     5581 cagttcgcaa ccgacattgg gccagacgag acaacagcaa cgctcagcga tcgcctggcc
     5641 gagctggcag cgcgtgagtt gcccagcgtc gcgctggcgt atctggctgg tgactccgcg
     5701 ccagtgccac aggacgacga tgcggctacc tacacacgcg agtggtcgac ggccgacgcg
     5761 ctgatcgact ggtcgcaccc ggccgatcag atcgagcggc tggtccgggc tgcgaacccg
     5821 tggccggtcg cctggacgac tgatggcgag acgcgcctca ggattctgac ggcacgtatc
     5881 gctgcgaccg ggaccggcca gccaccaggt acggtcgcga tcgtgaacgg acgagtcgtc
     5941 gtcgcaaccg gggccggcga cctgcacctc gacctcgtcc agccggccgg caagcgcgcg
     6001 atgccggcgg ccgactggtg gcgaggaacg cgacgagcga cggccgtact cggcacgtcg
     6061 ctcgatcgca gctaatcacc ccttgtcgtc ctgagccgca gcgaaggacc tccccgcgcc
     6121 agcctcatgg gcggtcacgg aagagaggtc cttcgctgcg gctcaggacg acggagtgcg
     6181 ttcactccta tgcgtcaggg gaagatcagt cgaccagata gatcatcggg atctgcgttt
     6241 gcacgtactc cacgaagcgc accgtcaggc cggtcgcgcc gctggtgatg aagcggtcgg
     6301 cgatgaactg cccctcgaac tgagtgccgg tcgccgcgcc ggccgtctgg accggcgagc
     6361 tgggcaggta gatcgtgccg cggctgactg agctggtgct gccggtccag tggaacgtcg
     6421 ccgtattggc gcggtccgag tacatgaaga caccgggata gtagccggag tagatcatgc
     6481 tgtcgggcgc cgtgaaccca aacgacgcgc tgccggacga actgagatag ccgccatcgc
     6541 agaagtagag gaagacctcg tcgccgctga tggtggagct acctgagttg aggaaccgcc
     6601 cgtggtcgaa gatgtattca ccggacggca gtgcaaagct cgtgctgccg ctcatctcga
     6661 gatcggacca gttcgagccg cttccaccca gaaagtaggc cttgacgtag acgccggagt
     6721 cgaacgtgat ccgcgagctc ccgctgaagg ccaggtccgc gccgttgacg aagtacatcg
     6781 tgtagttgcc cgaactgaag tgcagccgat cgctgccgga catccgcagg ccatcgccgc
     6841 cgttgaagat gtaggtaccg ggtcctatcg tcagcttgtt gctgccgccg gtgtggatct
     6901 cgctgccacc gccgaagtag aggttcccgc caccgatgat gttgttgttc gcgctgccac
     6961 cgatgttgat gccggtgccg ccgttgaacg agtagttgcc ggacttcatc tcgaagtagg
     7021 gcgtggcgcc accgatgttg atgccgccgc tgctgccgtt gaagttgtag gtgccaccct
     7081 ggaggacgat atcgcccccg ttgccaccgc cgacggtaat accggctccg ttgtcaaagc
     7141 ggtagctgcc gctctggagc gacatcgtct gcccgcccgg gatgttctgg atgttgacgc
     7201 aactggagcc gccgctggtg tatttcgatg acgtgatcgt gtacgtgacc ggattcgacc
     7261 acggcgagta ggtgcggcag ttgccgttgg ccgggttcac ggttccaacc ggattggacg
     7321 gaacactcgg gacagtcggc gggtcgatct tgtcggcgag cgggtcagga acctcggcac
     7381 cgtgcggctt gacgccgctt tcgccgttga tgttggtcga gccggatgta ttgaatccgt
     7441 cattggcaac gacgtaggcg gagagggggc tggggcaacc ggagcctccc tgagctgcga
     7501 tcgtcgtcga gccgctggtc ctgatctcat agtttgagac gatgctgccg cagatcgccc
     7561 ggatggatgt tgcgccggac gtgttgatgc caccggcgtc ggagttgagc gccagaatgg
     7621 cggcgtcgaa gccctccggc tcgagaccgg cgatcgcgga ggcgctcact tcccacggac
     7681 cggtgtagat cgcgccgata aagaagcgat cgacctcctt gttgacggtt acctggacat
     7741 aattggagtc accggcatag gcgcttccgg ccgccggcgg ccagttgacc gtgacatcgt
     7801 cggccgagac gccggcattc tccgctgcgt aggcgacggc cgcggctgtc acgtcgccag
     7861 aactcagatc gtcctggatc gagacggcgg cggccagcgc ggcagcgtcg gctgcgttct
     7921 gaacctggcg acgctcggcc atcaggaagc cggtgtcgat ggataacgca atgaagccga
     7981 ccagcaccag gctggccagc gcgaagatga tcatgacctg tccgcgcacg gcggatcgca
     8041 tctcaggtcg acaggatcgc ttgcggttca atcgtgccag cttcatcact catcctcgct
     8101 tctgcttcga tctgccgttc agaaccggtg ccctcaattc ggagggcagc acaacacacg
     8161 atcaatgcgc ccgccggtca tagcccgcaa gacgctttgt cctatcgtat ggagatgagc
     8221 cgggaattgg tggatgcaaa agtcacggtt ggctctagta ccaccggttg aatgcgggtt
     8281 tcggcctgat ttttcgtccc tgcgtagagg cgatggccgc agcctcgctc cctacgcgaa
     8341 ccgcacatcg gaagcgggcg agtgaaggtt ggctgacgat gcggtgggga tcgactgacc
     8401 gtcgacggtg tagatcgtca gattgtcgaa gctgacggtc gtggtcgcct cgctctgact
     8461 ctcggcgtgg acgccccatg cgccgatctg gacggttgag tcctgcgtcg cgccgatgag
     8521 ccggttgttg acgacgaact cgatccggtc ctcgacgacg gtcaaccgca ggcgctggct
     8581 ggcgccggcc tgatagccct cgatcggacc gaagaacaga tcgcgatact cgccgccgtg
     8641 ggacgcgacg cagccggccc agccagtgtc gtccatcagg cagacgtaca tcgagtcgtt
     8701 gccatccgca tcgaccgtcg agcgcgcgac cagcccggca tagccggccc cggacagcgt
     8761 cacgtctgcc tcgatcacgc cgttgcgtgg atgcggcatc ggcccgggcg tgatgacttg
     8821 ccattcgccg tcgtcgacat ccacgacata acgtccgtca gagatcatcg cgtgtgcgcc
     8881 ggagtccata tcaccgacgg caaagagctg cccggcctgg tcctcaaacg tcgtcgtgta
     8941 cagcgccgtc agcccatccg gctcgtcgac aggcgttgac tgtccggccg gcgccgttcg
     9001 atcggccacg gtcgctgacg gctcaacgtc ggattcagtc tgacgatcgg acccgcaggc
     9061 aaccaacgcc aggacgacag acaacgcgca acacaggcgg ccaaagcgat tgaagaccat
     9121 cacacatttc cacaagccgg gtaactattc ttgggaattg tatcgcggcg ttgccatctc
     9181 ccgcccgcag gacccgtaga cccatgctgg acgcaaatgc gtacaagtgc agttatccgc
     9241 aatacgctcg tcgccgaggc atcatcggtt aacgtcgttc ccctgtcccg ggtcgcctca
     9301 gcgcgtttct atttcggggc aggcaacgtc cagatctgcg atcaacagta gcgttttgac
     9361 gcagcatctg ctatgacgct tcgtgtacaa cgatatgcgc ggccgacctt acgtcatact
     9421 ctgggatcat gcgccgtgga caaccccgtt acgacaatcg actggcccgc agacgtcgcg
     9481 ttgacgcacc tgccgttcgt cggtcgcgac gccgaactgg cggcgtccga agaggcacat
     9541 ggccgtgcga tcgcgggtgc tgggcaactg gtactgctgt caggagcggc aggcatgggc
     9601 aagacccgcc tggcagaaca agtcgccacg atcgcgcgaa gccacaatgc gatggtggcc
     9661 tggggcagct gtcacgagtg ggagggcgcg ccggcctact ggccgtggac gcagatactc
     9721 cgcacctgca tccgtgtcca cggtactgaa atgcttcctc agttgtcgcc agttcagcgt
     9781 gaccttctca atcagatcgt acctgagttg caggatcacg atcgcgtgcc ggaccggctc
     9841 ggccatctcg agcccgaggc tcagttattt caggtgttcg ccgccaccag cgcgttggtg
     9901 cgccacgtcg ctgaccacca acccttggtc atcgttctcg acgacacgca ctgggcggac
     9961 acgccgacgc tgcaactgtt gcgctttctg gcggtcgata ttcgagaagc cgcggtcctc
    10021 attattgcaa cctaccgctc agacgaactt ggcccgtcgg atgcggtact gtcattgctg
    10081 actgatttgt acggagagac gcactgtctc cgtctggcac ttcggggcct ggcgaaacat
    10141 cacatcgcgg acctggttgc tcttgtcgcg gctgaggcac agccggcgcc acttgtcgac
    10201 gccatcttcg aggagacaga tggcaatccg ttctttgtgg ccgaggtcgt tttgctgctg
    10261 gcccaagagg gatcgttggg cgcgtcggcg gttacagcac gccggctgca agtaccggca
    10321 aatgttcgcg cagcgatccg gcaacgggtg gcacgtctat ctccggattg tcgccaggtg
    10381 ctgacagctg catccgttat cggacgagac gtcaatattc ggattcttgc acgcgccacc
    10441 cagttggcag ccccggtatt gatggacgcc ctcgatgatg ccagccgggc gcagttgatc
    10501 gtgccgggcg acagtccacg caccttccga tttgttcacg cgctcgtaca ggagtcgctg
    10561 taccaggagc tgaccggctc ggaacgggtc cggttgcatc tggcgattgg agccgcgctt
    10621 gaagcgagcg ttgacgtcga accgcattgg gaggaactcg cccaccacta cgtccaggca
    10681 gggccgtttg gcgacccggc caagacgctc cagtacaccg agcaggcggg gctcgtcgcg
    10741 atgtcacatt tcgcgtggca gaccgcagca aaccactttg cccacgcgct tgacgctctg
    10801 gaattgagca acccctcgga cactgcccga cgttgccatc tcctgctggc tctcggagat
    10861 gcccagaacc gatccggacc tggctcgggc gattctccca cggcccgtgt gagctttctg
    10921 gaggcgttcc ggctcgctga atcgcggggc gatcacacgt caatggcaca ggcagcgatt
    10981 gggttcgctg gactgaacgt cgtcaccgcg ttcggtgctg agcagcagga actgcttgag
    11041 tgttctctcg cggcgctccc aacaacagat agttcactgc gagtgcgggt gctgagccgg
    11101 ctggcggtag atctgtggaa ccgatccact gccagcctca ggcgcgcaag cggccttgtc
    11161 gacgaagcgt tcgcggtcgc aactcgcatg gacgatgtcg ggctgcagag cttcgcgtta
    11221 tgggcgcgac actacagtgg ctggcggcca gacaatcttg atgagcgaat cgccgttgca
    11281 tcacggctta ttgccctttc cgagcagacc ggcgatcctg ttgtgaccgc ctgggggtac
    11341 atctcacgga cgctcgactg tgttgaagcg gctgacttgt ttgccgcatc ccaatccctg
    11401 acgatattgg ggcagtttgt cgaacgtgtc catatcccct acgtcgcgct gcgtgagacg
//
LOCUS       DASUGJ010000342         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1440822, whole genome shotgun sequence.
ACCESSION   DASUGJ010000342 DASUGJ010000000
VERSION     DASUGJ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440822"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VFV93_07610"
     CDS             <1..815
                     /locus_tag="VFV93_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX5165244.1"
                     /translation="LVLVVRAFEDSNVPHPEGNVDALRDLETLFLEFTFSDLAIIEKR
                     LVRIQATIPKLRGNEKEAHEREAGALERCKVALEDGTPIRDVELDPEEERLLRGFGFL
                     TQKPVLVLLNVGEEQLGEPMEALTAQAREQFGRSGVEIHALAGKIEMEIAQLDDEDAQ
                     LFMADLGINESSRDRVVRISYGLLGLISFFTVGPDENRAWTIPNGATAVEAAGEIHSD
                     IARGFIRGEIVAYDDMIATRTMAEARKAGKLRLEGKTYVIQDGDIAHFLFNV"
     gene            1239..>2720
                     /locus_tag="VFV93_07615"
     CDS             1239..>2720
                     /locus_tag="VFV93_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165245.1"
                     /translation="MPVDALTSVRTGPLLPWWGVVLLGLAIGAVALAAMRPALRRRWP
                     WLLPEVAAFAALALLHLLFFWQPYRTGAVVPKGGGDLASFFYPIHAFAAREVQDGRLP
                     FWNPHLFSGAPHLANFQAGLLYPPNLLAYLLFDPFDYAALELLVILHFLLASWGAYWL
                     ARVYGIPRDGAVLTGALYAYSGFMVAHLGHYPMLATAAWAPWLLAAIVATIRRDSWLA
                     ALLGAPTLAMAVLAGHQPILLFTLTGVVAVAVFELWRAWGLGIRGSGETHATRLPRFP
                     VPESRIPKLATTALLALGLSAPAVLPMLELTGRTVRSGLEYTDASQFSVEPVALIHLL
                     LPTVFGSNATDYWGAFSNTEIWGYTGVMAMVLAAFGLVVRPTRTRIFWGVAAIVALLF
                     LLGPFASLHGWAYAFLPGYDRVRGAGRAYMFFDLAVALLAGWGLGALLRERSRWSAQQ
                     EIVLRWGILGLAGVLAVVAGLLIPLLTVSVVSDPDGYNRPVIAL"
BASE COUNT          393 a          931 c          895 g          501 t
ORIGIN      
        1 cgctggtgct ggtcgtccgc gccttcgagg acagcaacgt cccgcacccg gaggggaacg
       61 tcgacgcgct gcgcgacctg gagacgctct ttctggagtt caccttctcc gacctggcga
      121 tcatcgagaa gcgccttgtc agaatccagg cgacgatccc gaagctgcgc ggcaacgaga
      181 aggaagccca cgagcgcgaa gccggcgccc tcgaacgctg caaggtggcc ctggaagacg
      241 gcacgccgat tcgcgatgtc gagctcgatc ccgaggagga gcggctgctg cgcggcttcg
      301 gcttcctgac ccagaagccg gtcctcgtgt tgctcaacgt cggcgaagag cagcttggcg
      361 agccgatgga ggcgctgacg gcccaggccc gcgagcagtt cggccgcagc ggcgtcgaga
      421 tccacgcgct ggccggcaag atcgagatgg agatcgccca gctcgacgat gaggacgccc
      481 agctcttcat ggccgacctg ggcatcaacg agtccagccg cgaccgcgtc gtccgcatct
      541 cctacggcct gctcggactg atctccttct tcacggtcgg tcccgacgag aaccgcgcct
      601 ggacgatccc caacggcgcg acggccgtcg aagcggccgg cgagatccac tccgacatcg
      661 cgcgcggctt catccgcggc gagatcgtcg cctacgacga catgatcgcg acccggacga
      721 tggccgaggc ccgcaaggcc ggcaagctcc gtctcgaagg caagacctac gtcatccagg
      781 acggcgacat cgcccacttc ctgttcaacg tctaggggcg ggattcggga ttcgggagac
      841 ggggttgtcc agacgccgtt atcattgcga tcccatgagc gaatgcaaac cagacacacc
      901 tgactgccaa acctgggagg cgcgaaccgt gcatgatcca cgtaggggcc gatcctgtat
      961 cggcccgcgc cgtgaggcgc cggttggaga caggatgcgc tggaaagggc cgacctgttt
     1021 gtctgtcatc ctgagccgca gcgaaggatc gcctggcgca cgtgatacga atacgtcgat
     1081 cggcagtcga gtgcgtcagt cctcacgtgc cgtcatcgtg tcaaggttga gcgatccttc
     1141 gcagcggctc aggatgacag acaaacaggt cggcccccgt gaaacgacca ccgttcgaga
     1201 cactggacgg atcgaattcc cgcagcatgt agcgtcatat gccggttgat gccctgacca
     1261 gcgtccggac cgggccgttg ttgccgtggt ggggcgttgt cctgctgggg ctggcgattg
     1321 gggcggtcgc gcttgcggcg atgcgaccgg cgctgcggcg gcgctggccg tggctgctgc
     1381 cggaggtggc tgccttcgcg gcgctggcgt tgctgcacct gctcttcttc tggcagccat
     1441 accggaccgg cgcggtcgtc ccaaaggggg gcggcgacct ggcgtcgttc ttctacccga
     1501 tccacgcctt cgccgcccgc gaggtccagg acggccggct gccgttctgg aacccgcatc
     1561 tcttctccgg cgctccgcat ctggccaatt tccaggccgg cctgctctat ccgccgaacc
     1621 tgctggccta tctgctcttc gatccgttcg actacgccgc gctggaattg ctggtcattc
     1681 tccacttcct gctggcgagc tggggcgcct actggctggc gcgggtctac ggcattcccc
     1741 gcgacggcgc cgtcctgacc ggcgcgctct acgcctactc cggcttcatg gtcgctcacc
     1801 tcggccacta cccgatgctg gcgacagccg cctgggcgcc ctggctcctg gccgccatcg
     1861 tcgccaccat ccgccgcgac tcctggctgg ccgccctgct cggcgcaccc accctcgcga
     1921 tggccgtcct cgccggtcac caaccgatcc tgctgttcac gttgacgggg gttgtggcgg
     1981 tcgcggtgtt tgagctctgg agagcttggg gactcgggat tcggggttcg ggagaaactc
     2041 acgctactcg ccttcctcga ttcccggttc ccgaatcccg aatcccgaaa ctcgccacca
     2101 ccgccctgct cgccctcggc ctctcggccc ccgccgtctt gccgatgctg gagctcaccg
     2161 ggcgaacggt gcgcagtggg ctggagtaca ccgacgcgag tcagttctcg gtcgagccgg
     2221 tggcgttgat ccacctgctg ctgccgaccg tcttcggttc gaatgcaact gactactggg
     2281 gcgccttctc gaacacggag atctggggct ataccggcgt gatggcgatg gtgctggctg
     2341 ctttcgggct ggtcgttcgt ccgacgcgga cgcgcatctt ctggggagtg gcggccatcg
     2401 tcgcgctgct ctttctgctg ggaccgttcg cctcgttgca cggctgggcc tatgccttcc
     2461 tgcccggcta cgaccgcgtc cggggggccg gccgcgccta catgttcttc gacctggccg
     2521 tcgcgctgct ggccggctgg gggctcggcg cgttgctgcg ggaacggtcg cgctggagcg
     2581 cccaacagga gatcgtgctg cgttggggca tcctcgggct ggccggtgtc ctggcggtcg
     2641 tcgccggcct gctgatcccg ctgctgaccg tcagcgtcgt cagcgatccg gacggctaca
     2701 accggccggt gatcgcgctc
//
LOCUS       DASUGJ010000343         6517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1303431, whole genome shotgun sequence.
ACCESSION   DASUGJ010000343 DASUGJ010000000
VERSION     DASUGJ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303431"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2329)
                     /locus_tag="VFV93_07620"
     CDS             complement(<1..2329)
                     /locus_tag="VFV93_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165246.1"
                     /translation="MRAISYRLIAALVSLTLAWVLVAQVVAAGATVPGNAAFERTWAR
                     TNKSVAEMRVSRTWIWGPSANTAAVKEEYAESPGGLRDVQYFDKARMEISQRNATDDG
                     LWYVTTGLLAMELVTGQLQLGDDLFEARTPANINVAGDENDSSGPTYATFTKLLDEPP
                     AKAGATLTQRLARDGTITKDDKFAKYGVTAAQHVEVPGIDHQIASPFWAFMNSSGEVY
                     VDGHYVNEPIFEDPYYAIGYPLSEAYWATIKVAGTSHDVLMQVFQRRVLTYTPANPAG
                     WQVEMGNIGRHYYAWRYAQLPTAPAAPTNVTLTASNPTGCTYSGGVATDCEYTLTLTW
                     KDASNNEDGFKISTSFDMTTYTAATNATTWSKAFRLQPGKAVILSVFAWNKVGNSAAV
                     ASNAVTLAAPTSVTVAAPSNVKLQISAPSDCDLTSGIATECDYTLTATWTDNSSNEDG
                     FEIRPDFSATTYKVAANVTTWSHEFSLEPREWVHVEVRAYNAAAQSNWVASENGYLHE
                     PTPAAPAEGTLVVSDPSDCVFVNGVATECAYAITATWKDQSNNEDGFRVRPSFTTTSK
                     RVDKDVTTWSGVFRLEPREWVSVEVQAYNSIGASAWVESEQVYLHEPTAPLAPTNVLL
                     AISDPSDCTIVNNVPVECTYTLTLTWTDGSKNEDGFKITTSFDTTTYTVPANTTTWSK
                     AFRLEPSKDVTLTIVAYNVVGSSKAIPSNKVTPLPSALEIPDAPKDVVLAVSTPYDCQ
                     GVSWLLTCKYDFTLTWKDMASDETSLTIRRLPDMIE"
     gene            complement(2571..5081)
                     /locus_tag="VFV93_07625"
     CDS             complement(2571..5081)
                     /locus_tag="VFV93_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX5165247.1"
                     /translation="MAARISHRVIPLLILVLLLLPAIPSSAAVPANDAFQRTWARTDL
                     PTSTDRVRRTWMWGPEAFTNAMTEDYAEAPEGQRVVQYFDKSRMEITNPDADKSSPWY
                     VTNGLLVVEMVTGRVQYGDAQFASLPPAMINIAGDLDDTSGLTYATIAALRNEPSATA
                     GALIVKRSDRAAIVKDDPSFADYGVRAAHRVTVPGIDHQIAEPFWEFMNSSGLVFEDG
                     VYRTDQLFEDPFYATGYPIAEAYWATIKVAGRERDVLTQCFERRCLTYTPDNPEGWQV
                     EAGNVGQHYYQWRYQTQPGTQYTLTNVVTLSPNYDTQMYMPRNVLVTRAGDVYVSDSG
                     NNRIQQYSSQGVFTKQWGYAGASYGSFSTPGGLAEDSHGNIFVADTGNARIQRFDSSG
                     NFQKAWSATGLIGPYDVSIDADDNLFVVDRDGESVWKFDTNGTALAAWGGHGSATGKF
                     NDPRGLGLDADGNVYVADRSNSRVQVFDNDGTFLRLANNDPQALLYAPDDVAIGASGD
                     VYVLDESAVAVTVYGSDGLGKKVWYSGSSSPQGIAIDADGDVWLASAGDYRVVEYSAD
                     GVPRNAWSFGSRGTISGPVAMAVAADGTIFVVDGTSTAQVFGPDGTFLSAWGGPGVFW
                     ALTDIELDPYGDVWVVDAGQHQLIRYHPVDGSYARMTETFRLGTDYSLGIPAAIAFDA
                     DDHIYIADQANARIVKLTRDGELITDWGSQGQGDGQFQTVNQIAVHGDSVYVADFGWS
                     GALRLHQFDLNGNFARQWHDVESLMMYQAALAIDADGFVYFADRDHYRIVKFTPSGTI
                     FTTIGEGNVTYPMVLAVAPDGHIVIGEEGALRTFEPAR"
     gene            complement(5195..5791)
                     /locus_tag="VFV93_07630"
     CDS             complement(5195..5791)
                     /locus_tag="VFV93_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165248.1"
                     /translation="MLPDPEWAYFAETGAHLRYGFRAFWDANGGLPVFGYPLTEEYRA
                     SNRDTGAIYTTQLTERQRFEWHPENAGTPYEVLLGRLGAEELIHQERDWTSLPTADPA
                     SPHYFPETSHAIAAEFWEYWSSHGLDVGDPGVSYRESLGLFGYPLSEPMVESNNEGAE
                     VLSQYFERAVFEFHPDNPAEYRVLLRRLGAEYLAARGW"
     gene            complement(6077..>6517)
                     /locus_tag="VFV93_07635"
     CDS             complement(6077..>6517)
                     /locus_tag="VFV93_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165249.1"
                     /translation="IAVIASDLATGKQVEVARNALCCAAVQPDGRVTWYATDGIWMRD
                     PRSDAPATLVWPKTESSTQFIGLLGDWAVRLQVIETEGKYSYYEHVQLVARQITTGES
                     TVLDDGWKWYDTQSFAGIVGTSIAYPRLYPSPADAERAGDWLAV"
BASE COUNT         1155 a         2015 c         2090 g         1257 t
ORIGIN      
        1 cttcgatcat gtcgggcagc cgtcggatcg ttaggctggt ctcgtccgat gccatgtctt
       61 tccacgtcag cgtgaagtcg tacttgcagg tgagcaacca cgacacaccc tggcagtcgt
      121 atggggtcga gacggccagc acgacatcct ttggcgcgtc cgggatctcc aacgcggacg
      181 gaagcggagt caccttgttg gaggggattg ctttcgacga gccgacgacg ttgtatgcga
      241 cgatcgtgag cgtcacgtct ttgctcggct cgagtcggaa cgccttcgac caggttgtcg
      301 tattcgctgg gacggtgtag gtcgtcgtgt cgaagctggt cgtgatcttg aagccgtcct
      361 cgttcttcga cccatccgtc cacgtcagcg tcaacgtgta cgtgcattcg accgggacgt
      421 tgttgacgat cgtgcagtcc gagggatcgg agatcgcgag tagcacgttg gttggtgcca
      481 acggcgccgt tggctcatgg agatagacct gctccgactc gacccaggcc gatgcaccga
      541 tcgagttgta cgcctggacc tcgacactga cccactcacg cggttcgagc cggaacaccc
      601 cagaccaggt cgtcacgtct ttgtcaacgc gcttgctggt cgtcgtgaaa ctcggccgaa
      661 cccggaagcc gtcttcgttg ttcgactggt ccttccaggt ggcggtgatg gcgtacgcgc
      721 actcggtcgc gacgccgttg acgaagacac agtcgctggg atccgagacg acgagcgtcc
      781 cctctgcagg agcggccggt gtcggttcgt ggagataccc attctcagag gcgacccagt
      841 tcgactgggc agcggcgttg taggcccgga cctcaacatg gacccactcg cgcggttcga
      901 gcgagaactc atgcgaccag gtcgtgacat tggcggccac cttgtaggtg gtcgccgaga
      961 agtccggacg gatctcgaag ccatcctcgt tgctggaatt gtcggtccat gtggcagtga
     1021 gcgtgtaatc acactcggtc gcgatgccgc tcgtcagatc gcagtcggac ggcgccgaaa
     1081 tctgcagctt gacgttgctc ggagctgcga cagtgaccga ggtcggcgct gcgagcgtca
     1141 cggcgttgga cgcgacagct gccgagttgc cgaccttgtt ccaggcgaag accgatagta
     1201 tgacggcctt ccccggctgg agccggaacg ccttcgacca ggttgtggcg ttcgtggccg
     1261 cggtataggt cgtcatgtcg aagctggtcg agatcttgaa gccatcctcg ttgttggagg
     1321 cgtccttcca ggtcagcgtc agtgtgtact cgcagtcggt cgccacaccg cccgagtagg
     1381 tgcagcccgt cgggttcgac gccgttagcg ttacgttggt tggcgcggcc ggggcggtcg
     1441 gaagctgggc gtaccgccag gcgtagtagt gccggccgat attgcccatc tcgacctgcc
     1501 agccggccgg gttggctggc gtataggtca gcacccggcg ctggaagacc tgcatcagca
     1561 catcgtgcga cgtaccagcg accttgatcg tcgcccagta ggcctcactg agggggtagc
     1621 cgatggcgta gtacgggtct tcgaatatcg gctcgttgac gtagtggcca tcgacgtaga
     1681 cctcacccga gctgttcatg aacgcccaga atggtgaggc gatctggtgg tcgattcccg
     1741 gaacctcgac atgctgggcg gccgtgacgc cgtacttcgc gaacttgtca tccttcgtga
     1801 tcgtgccgtc ccgcgcgaga cgttgcgtca gcgtcgcgcc agccttggcg ggcggctcgt
     1861 cgagcagctt ggtgaacgtc gcgtatgtcg gcccgctaga gtcgttctca tcacccgcga
     1921 cgttgatgtt tgcaggcgta cgcgcctcga acagatcgtc accaagctgc aactgaccgg
     1981 tgacgagttc catcgccagc aggccggtcg tgacgtacca cagaccgtcg tcggttgcgt
     2041 tgcgctgtga aatctccatt cgggccttgt caaagtactg gacgtcgcgc aagccgccgg
     2101 gagactcggc gtattcctcc ttgaccgcgg cggtgtttgc gctcggtccc cagatccagg
     2161 tacgagacac gcgcatctcg gcgacggact tgttggttcg tgcccaggtg cgctcgaagg
     2221 cggcgtttcc cggcacggtt gcgccagcag ccaccacctg cgccacgagg acccaggcga
     2281 gtgtcagcga gacgagagct gcgatcaggc gatacgatat cgcgcgcatc agctcccacc
     2341 ctttctatcg gccgccccaa tggcgtcgtc ggcatacaag ggcggcgaag gcacatatga
     2401 tggcgacgca tctgcattgt gtaggggaat ccgtgcacat gacgtaatga acgggcgatg
     2461 ggcgctacaa ttctgtgtca gatggaccag acatagtttg ggcgccagct gtcctcaaca
     2521 actggcgccc gcatcctcaa acgattcgat gggtccgatc acgcggcgag tcagcgtgcc
     2581 ggttcaaagg ttcgcagtgc tccttcctcg ccaatgacga tgtgcccatc gggggcgacc
     2641 gccagcacca tcgggtaggt gacgttgcct tcaccaatcg tcgtgaagat ggtgccggat
     2701 ggcgtgaact tgacgatccg gtaatggtcg cggtcggcga agtagacgaa gccgtcggca
     2761 tcgatcgcca gcgccgcctg atacatcatc agcgactcga catcgtgcca ctggcgggcg
     2821 aagttaccgt tcaggtcgaa ctgatgcagc cgaagcgccc ccgaccagcc gaaatcggcg
     2881 acgtagacgg agtcgccatg gacagcgatc tggttgacgg tctggaactg tccgtcgccc
     2941 tgtccctgcg acccccaatc cgtgatgagt tcgccatcgc gggtgagctt cacgatccgc
     3001 gcgttcgcct ggtcagcgat gtagatatgg tcgtcggcgt cgaacgcgat cgcggcaggg
     3061 atgccgagcg agtagtccgt gccgagccgg aatgtctccg tcatccgcgc gtaggagccg
     3121 tcgaccggat gatagcggat caactggtgc tggccggcat cgacgaccca gacatcgccg
     3181 tacggatcga gttcgatatc agtcagcgcc cagaagactc ccggcccacc ccaggcgctc
     3241 aggaacgtcc cgtccggacc aaagacctga gcggtcgatg tgccgtcgac gacaaagatg
     3301 gtcccatccg ccgcgaccgc catcgcgaca gggccggaga tcgtgccgcg tgatccgaac
     3361 gaccacgcgt tgcgggggac gccgtcggcc gagtactcga cgacacgata gtctcccgcc
     3421 gacgccagcc agacatcgcc gtctgcgtcg atagcgatgc cctgtggcga ggaactgccg
     3481 gagtaccaga ccttcttccc caggccatcg gatccataca cggtcacggc caccgcgctc
     3541 tcatcgagga cgtagacatc gccggatgct ccaatggcaa catcgtccgg tgcgtacagc
     3601 agggcttgcg ggtcgttgtt cgccagtcgc aagaaggtgc catcattgtc gaacacctga
     3661 acgcgcgagt tgctcctgtc ggcgacatag acattgccgt cggcgtcgag tccgaggccg
     3721 cgcggatcgt tgaacttgcc ggtcgcgctg ccatgcccgc cccaggcagc cagcgcggtc
     3781 ccgttcgtat cgaacttcca gacgctctca ccgtcgcgat cgacgacgaa gaggttgtcg
     3841 tccgcgtcga tggagacatc gtacggtccg atcaggccgg tcgcgctcca tgccttctgg
     3901 aagttgccgc tgctgtcgaa gcgttgaata cgcgcgttgc cggtatcggc aacgaagatg
     3961 ttgccgtggc tgtcctcggc caagccaccg ggcgtggaga aggacccgta ggaagccccc
     4021 gcgtaccccc actgcttggt gaagacacct tgggaactgt actgctggat gcggttgttg
     4081 ccgctatcgg agacgtagac gtctccagcg cgcgtgacga gcacattgcg cggcatgtac
     4141 atctgggtgt catagttcgg cgaaagtgtg acgacgttgg tcagggtgta ctgtgtaccc
     4201 ggctgcgtct gatagcgcca ctggtaatag tgctggccaa cgttgccggc ctcgacctgc
     4261 cagccttcag ggttgtcggg tgtgtacgtc aggcagcgcc gctcgaagca ttgggtcagc
     4321 acgtcgcgct cgcgaccggc gaccttaatc gtcgcccagt aggcttcggc gatcggatag
     4381 ccggtggcgt agaacggatc ctcgaagagt tggtcggtcc ggtagacgcc atcctcgaag
     4441 accagcccgc tggagttcat gaattcccag aacggctcgg cgatctggtg atcgatccca
     4501 ggaacggtga cacggtgagc agcccggaca ccgtagtccg cgaatgacgg atcgtctttg
     4561 acgatcgcgg cccgatcgga tcgcttcacg atcagcgcac cggcggtcgc agatggttcg
     4621 ttacgcaggg ctgcgatcgt cgcataggtg aggccggagg tgtcgtccag gtcgcccgcg
     4681 atgttgatca tcgccggtgg gagcgaagcg aattgcgcgt caccgtactg gacccggcca
     4741 gtgaccatct cgacgaccag caggccgttg gtgacgtacc aggggctcga cttgtccgca
     4801 tccggattgg tgatctccat ccgcgacttg tcgaagtact ggacgacccg ttgtccctct
     4861 ggggcctcgg cgtagtcctc tgtcatcgcg ttcgtgaacg cctccggccc ccacatccag
     4921 gtccgcctga cgcgatccgt actcgtcggc agatcggttc gggcccaggt tcgctggaag
     4981 gcgtcgttgg ccggcaccgc tgccgaagaa gggatggcgg gcaggagcag caacacgagg
     5041 atgagcagtg gaatgactcg atgagatatg cgggcagcca tggctgcctc ctctcgttcc
     5101 cgacgctcgc gcgttcatgg cgccttcgcg ggtctacagc agatgccgaa tcgcgcctcg
     5161 tggatgcagg tcttgcctcc tctcttcggt ggctttacca gccacgcgca gccaggtact
     5221 ccgctccgag ccgccgcaac aacacccggt actcggctgg attgtccggg tggaattcga
     5281 agacagcccg ctcgaagtac tgtgagagca cttctgctcc ctcattgttg gactcgacca
     5341 tcggttcgga gaggggatag ccaaacagcc cgagcgactc ccggtaactg acaccgggat
     5401 caccaacgtc cagcccgtga gacgaccagt actcccagaa ctccgccgcg atcgcgtggc
     5461 tggtttcggg gaagtaatgc gggctggctg ggtcggcggt gggtaacgag gtccagtccc
     5521 gctcctggtg gattaattcc tcggcgccga gccggcccag cagcacctca tacggggtgc
     5581 cggcattctc cggatgccat tcgaagcgct ggcgctcggt gagctgggtc gtgtagatcg
     5641 cgccggtgtc gcggttgctc gcacggtact cctcggtcag cggatagccg aagaccggca
     5701 agccgccgtt tgcgtcccag aacgcgcgga acccgtagcg gagatgtgcg ccggtctcgg
     5761 cgaaatacgc ccattccgga tcagggagca tgtcgtcgat catcagtccg acgacggcgg
     5821 gtgatccgtc cggtagcaca tcggtccagg ccagcagccc gttgccatag gccggccaga
     5881 ctggggattc ctcactgagc gtcagcgtga aggcgctgga gcgaacgaga tcggtgaccc
     5941 acagatccgc tcccccgatg gcctgctcgg tgcggcactt cagcagcagg aagtggtcgt
     6001 caccgacaat ccgggtgttc agatagaagc aggccgctgg acgctggaag taaccaggcg
     6061 ccgccccgtc gagcttctac acagccagcc agtcgccagc ccgctcggca tccgccgggc
     6121 ttgggtacag gcgcgggtag gcgatggacg tgccgacgat tccggcaaag ctttgcgtgt
     6181 cgtaccattt ccagccgtcg tccaagaccg tggactcacc ggttgtgatc tggcgtgcca
     6241 caagctgaac gtgctcgtag tacgagtact tgccctctgt ctcgatgacc tgaagcctga
     6301 ccgcccagtc ccctagcaga ccgatgaatt gtgtcgatga ctcggttttc ggccagacca
     6361 gcgtcgccgg ggcgtcgctg cgaggatcgc gcatccagat gccgtcagtg gcataccagg
     6421 tgactctccc gtccggctgt accgcagcgc agcacagcgc gttgcgcgcc acttcgacct
     6481 gcttcccggt tgccagatcg ctcgcgatga ccgcgat
//
LOCUS       DASUGJ010000344         4585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_471185, whole genome shotgun sequence.
ACCESSION   DASUGJ010000344 DASUGJ010000000
VERSION     DASUGJ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471185"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VFV93_07640"
     CDS             complement(<1..911)
                     /locus_tag="VFV93_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5165250.1"
                     /translation="MYHRYDALVIGAGGAGLMAAAQLSHSCSTAVISKLYPPRSHTGA
                     AQGGIGAALGNVEEDNWLWHMFDTVKGGDYLTDQDAAEVLAREAIDAVYDLEHMGLPF
                     NRTADGKIDQRRFGGHTRNYGEGPVRRACYSADRTGHMILYTLYQQGIKNDVEFYDEY
                     MMLDLLITDDGICNGVVAMNVADGEIHVFHAKAIILATGGFGRVFKVTSNAMAATGDG
                     VAVAFRRGVPLEDMEFYQFHPTGIYKLGILLTEGARGEGGILRNSSGERFMERYAPHA
                     KDLASRDVVSRSMTIEIREGRGCGPRK"
     gene            complement(916..1326)
                     /locus_tag="VFV93_07645"
     CDS             complement(916..1326)
                     /locus_tag="VFV93_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX5165251.1"
                     /translation="MQSRGGGRARPAEGNQIEVFSWYFFRVSGLLLIFLAIAHVVIMH
                     VVNTVDEIDYAFVADRWGSPFWKVYDFLLLSLALLHGINGGRVGIEDYVRSNGWRTVA
                     MTTLWITAFVFLVIGAMAIVTFDPVAFKAAHAGS"
     gene            complement(1350..1706)
                     /gene="sdhC"
                     /locus_tag="VFV93_07650"
     CDS             complement(1350..1706)
                     /gene="sdhC"
                     /locus_tag="VFV93_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX5165252.1"
                     /translation="MQRVYRGREGMISWALHRLTGLGVLLFLLIHIADIYVISYGPEE
                     FDDLLFIYNSLGFRLMEILLVGALYYHAINGVRIILIDFWDSAVLIQRQLWYGGIVLF
                     FLTFIPTAILMARPLF"
     gene            1999..3150
                     /locus_tag="VFV93_07655"
     CDS             1999..3150
                     /locus_tag="VFV93_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5165253.1"
                     /translation="MTPSQRQLERVRATAFELLDGVAKRAVSICQVPAPTGAEDERAA
                     YVASLLTGLGYTPERDDAGNVYARRGKGSGKVVLLAAHTDTVFPAGTDVSVRRDGERL
                     HGPGVGDNSLGVAAMLGVLELLDNLSIETDADIVVAATVGEEGLGNLKGVRAAVERYR
                     PELGAVVAVEGHNLGRVTHAGVGSTRWRIRVSGPGGHSWGAFGKPNAIHGLSRIINEI
                     SRLDVPGDPKTTYNIGTIEGGTSVNTIAPGASALLDMRSVDAGSLKRLVDRVGSIVAT
                     AAGDGLTVDVDVLGERPAGSCPKNDPLVVAAGDVLRQVGFEPVFDASSTDANIPISLG
                     IPAVCIGITHGGLGHTVNEFIDIPPIATGLTQLGLLSIAAASIVAGGQA"
     gene            3164..>4585
                     /locus_tag="VFV93_07660"
     CDS             3164..>4585
                     /locus_tag="VFV93_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX5165254.1"
                     /translation="MLSDVCVALDVEATGMDPTRDEVIEIGAVKFRGDKIIDRFEELV
                     RPAGPISLGIQTLTGLTNDELRRAPLFAAVAPKLRDFVRQAPIVGQSVEMDLDMLQAA
                     GLRFPNVRYDTFELATVLLPELPAYNLGTIASVLGVEVSSKHRAVADAETTMAVFNQL
                     VLRADQFDDATLERLIGVTRLSGSHLTRFFTSLLRERRSEPGLAGTSIGAKLLEQLSS
                     APELGSEAMFLMPRERPERLELTGSGDPVPPAALDAAMEADGAFSRTVPGFEQRPQQL
                     QMMHRVAEALNEGGQLLVEAGTGTGKSLGYLLPAALHAVEQGDRVVVSTATIALQDQL
                     LKKDVPALKAAAEQADPNGADAALVPLRDLKVAVLKGRANYLCLRRWFIAQREDATSP
                     AQAQLYAKIIAWLQQTNTGDSAELHLSPDQRGYWGRLAEEEGSCIPGQCVFHRRNQCF
                     LFRARQEAEAAHIVIVNHSLLISD"
BASE COUNT          799 a         1423 c         1553 g          810 t
ORIGIN      
        1 tccttacgcg gaccgcaacc gcgtccctcg cggatttcga tggtcatgga gcggctgaca
       61 acatcacggc tggcgagatc cttggcatgc ggcgcgtagc gttccataaa gcgctcgccg
      121 gaactgttgc gcaggatgcc gccctcgcct cgcgcgcctt cggtcagcaa gatgccgagc
      181 ttgtaaatgc cggtcgggtg gaactggtag aactccatgt cttccagcgg gacgccacgc
      241 cgaaaggcga ccgcgacacc gtcgccggtg gccgccatcg cattggaggt caccttgaag
      301 acgcggccga agccgccggt cgccaggatg attgccttgg cgtggaagac gtggatctcg
      361 ccgtcggcga cgttcatagc gacgacgccg ttgcagatgc cgtcgtcggt gatcagcagg
      421 tcgagcatca tgtactcgtc gtagaactcg acgtcgttct tgatgccctg ctggtagagc
      481 gtgtagagga tcatgtggcc tgtgcggtcg gccgagtagc aggcgcggcg gaccgggccc
      541 tcgccgtagt tacgggtgtg gccgccgaag cggcgctggt caatcttgcc gtccgcggtg
      601 cggttgaacg gcagccccat gtgctccagg tcgtagacgg cgtcgatcgc ctcacgggcc
      661 aggacctcgg cggcgtcctg gtcggtcagg tagtcgccac ccttgacggt gtcgaacatg
      721 tgccagagcc agttgtcctc ctcgacgttg ccgagggcgg caccgattcc gccctgggcg
      781 gctccggtgt gcgagcgggg cgggtagagc ttcgagatga cggctgtgct gcacgagtgt
      841 gagagctggg cggcagccat caggcccgcc ccgccggcgc caatcacgag tgcgtcgtat
      901 cggtggtaca tcgcgtcaac tccccgcatg ggcggccttg aaggccaccg ggtcgaacgt
      961 tacgatcgcc atcgcgccga tgaccaggaa gacgaaggcg gtgatccaga gggtagtcat
     1021 cgccaccgtt cgccagccgt tactccgaac gtagtcttcg atgccgaccc ggccgccgtt
     1081 gatgccgtgt agtagcgcca gcgacagcag aaggaagtcg tagaccttcc agaagggtga
     1141 gccccaacga tcggcaacga aggcataatc gatctcatcg acagtgttga caacgtgcat
     1201 gatgacgacg tgcgcgatag cgagaaagat cagcagcaga ccgctgaccc ggaagaagta
     1261 ccacgagaag acttcgatct ggttcccctc ggccggtcgc gcccggcctc caccacggct
     1321 ctgcatgaat cgttcccctg tcacctttcc taaaacagtg gccgggccat cagaatggcg
     1381 gttggaatga atgtcagaaa gaagaggacg attcctccgt accagagttg gcgctggatg
     1441 aggacggcgc tatcccagaa gtcgatcagg atgatgcgca cgccgttgat ggcgtgatag
     1501 tagagcgcgc cgacgaggag gatctccatc agacggaagc cgagactgtt gtagataaac
     1561 agcagatcgt cgaactcttc ggggccgtaa ctgatgacgt agatgtcggc gatatggatc
     1621 aacaggaaga ggagcactcc cagaccggtg agccggtgga gagcccacga gatcatgcct
     1681 tcacgaccgc gatacacccg ctgcatcaac cctcctcctc aacgccagac gctcggcgcg
     1741 catcgcggag gcgaggatgt cgtggtgtta gccggacgat cgcgcgcgaa gccgatgcca
     1801 ctgtacggag catgccaaaa cgtgtcaagg aattggcttc agtttcacta ttttggtgta
     1861 tctcagccgt cggtcggggc gggcatgcta tactttggtg cctgacggca cacccggatt
     1921 cgattgaatt cgcgctgtat ttgcctcgac gagccgtcgg ctgcaatcac gccgattctg
     1981 tcagtccctg gaggacactt gacgccatcc cagcgccagc ttgagcgtgt gcgggcaacc
     2041 gcgtttgaat tgctcgacgg tgtcgccaag cgcgctgttt cgatctgtca ggtgccggcg
     2101 ccgaccgggg ccgaggacga gcgcgccgct tacgtcgcga gcctgctgac ggggctgggc
     2161 tacacgcccg agcgcgacga tgccggcaat gtctacgccc gacggggcaa gggttccggc
     2221 aaggtcgttc tgctggcggc ccacaccgac acggtctttc cggcgggaac cgatgtcagc
     2281 gttcgccgcg acggggaacg gctgcacggt ccgggcgtcg gcgacaacag cctcggcgtc
     2341 gcggcgatgc tcggcgtgct ggaactgctc gacaacctct cgattgagac cgatgccgac
     2401 atcgtcgtcg cagccacggt tggcgaagag gggctgggca acctgaaggg tgtccgtgct
     2461 gccgttgagc gctaccggcc ggaactcggc gcggtcgtgg ccgtcgaggg gcacaacctc
     2521 gggcgtgtca cgcacgctgg tgtcggctcg acgcgctggc ggatccgcgt cagcgggccg
     2581 ggcggtcatt cgtggggcgc gttcggcaag cccaacgcca ttcacggttt gtcgcgcatc
     2641 atcaacgaga tctcccggct cgatgtgccg ggtgatccga agacgaccta caacatcggc
     2701 acgatcgagg gcggcacgtc ggtcaacacg atcgcgccgg gtgccagcgc gctgctggac
     2761 atgcgctcgg tcgatgccgg ctcgctcaag cggctggtcg accgggtcgg ctcgatcgtc
     2821 gcgacggcgg ccggagatgg gctgacggtt gacgtcgatg tgctgggcga acggccggcc
     2881 ggcagttgcc cgaagaacga tccgctggtt gtggcggccg gcgatgtcct gcgacaggtc
     2941 gggttcgagc cggtcttcga cgcgtccagc accgacgcga acatccctat cagcctgggg
     3001 attccggccg tctgtatcgg gattacacac ggtggcctcg ggcataccgt caacgagttc
     3061 attgatattc caccgatcgc gaccggcctg acgcagctgg ggctgctgtc gatcgcggca
     3121 gcgtcaatcg tcgcgggcgg ccaggcgtga ccggacggcg ccgatgctga gcgatgtctg
     3181 cgtcgcgctc gacgtcgaag cgaccgggat ggaccccacc cgcgacgaag tgatcgagat
     3241 cggcgctgtc aagtttcgtg gtgacaagat catcgatcgc ttcgaagagc ttgtccggcc
     3301 ggccggaccg atctcgctcg gcatccaaac gttgacgggt ctaacgaatg acgagctgag
     3361 gcgagcgccg ttgtttgccg ccgttgcgcc gaagctgcgt gatttcgtgc gccaggcgcc
     3421 gatcgtcggc cagtcggtcg agatggacct cgacatgctc caggccgccg gcctgcgttt
     3481 cccgaacgtt cgctacgaca cgttcgagct ggcgacggtg ctactgccag aattgccggc
     3541 ctataacctc gggacaatcg cctcggttct cggcgttgag gtttcgtcca agcaccgggc
     3601 ggttgccgac gccgagacga cgatggccgt cttcaaccag ctcgtgttgc gggccgatca
     3661 gttcgacgac gcgacgttgg agcggttgat cggcgtgacg cgcctctccg ggtcgcacct
     3721 gacgcgcttc ttcaccagcc tgctgcgcga gcggcgctcg gagcctggcc tggccgggac
     3781 atcgatcggt gccaagctgc tggaacagct ctcgtccgcg ccggagcttg gctcagaggc
     3841 gatgttcctg atgccgcgcg agcggcccga gcggctggag ttgacgggca gcggtgatcc
     3901 ggtcccgccg gccgcgctcg atgccgcgat ggaagcggac ggcgcgttct cgcgaacggt
     3961 accgggcttc gagcagcgcc cgcagcaact gcagatgatg catcgggtgg ccgaggcgct
     4021 caacgagggc gggcaactgc tggtcgaggc cgggaccggc accggcaagt cgctcggcta
     4081 cctgttgccg gctgccctgc acgcggtcga gcagggcgat cgcgtcgtcg tctcaacggc
     4141 gacgatcgcg ctccaggatc aactgctcaa gaaggatgtc ccggcgctga aggcggctgc
     4201 cgagcaggcc gatccaaacg gcgcggacgc ggcgctggtg ccgctgcgcg atctgaaggt
     4261 tgctgtcctg aaggggcgtg ccaactacct ctgcctgcga cgctggttca tcgcccagcg
     4321 ggaggatgcg acctcgccgg cccaggcgca gctgtacgcc aagatcatcg cctggttgca
     4381 acagacgaac acgggcgaca gtgccgagct gcacctctcg ccggatcagc gcggctactg
     4441 gggcaggctg gccgaggaag agggctcatg catcccgggc cagtgcgtct tccaccgtcg
     4501 caaccagtgt ttcctgttcc gtgcgcggca ggaggctgaa gcagcccaca tcgttatcgt
     4561 caaccactcg ctgctgatct cggat
//
LOCUS       DASUGJ010000345         9157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1859001, whole genome shotgun sequence.
ACCESSION   DASUGJ010000345 DASUGJ010000000
VERSION     DASUGJ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859001"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2216)
                     /locus_tag="VFV93_07665"
     CDS             complement(<1..2216)
                     /locus_tag="VFV93_07665"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5165255.1"
                     /translation="MLTTLESRQLLVQTRIHPPLLRAQRISRTRLIERLNAAVTRLTL
                     IVSPAGYGKTTLLTQWARQREEPVAWLTLGADDNVLSVFLNYLLATIGEVDHDLADAV
                     SHLGTGDDPGDAKSVFAAILSQLSAHTEPWILILDDYHVITNNEIDQCVTQLIEGTPP
                     ECRIIIASRSEPALPLAQLHVRQELTRLNANDLRVTSAELPELLSSYGIVASASESLA
                     LNEQTNGWIAALHLAAIHATEYSLESVIRSLRSFDGDVLMLGDYLLQEVLNQQHFEMR
                     EFLLRSSILDRFTAQTCRTVTRLRRAGQLLDLAVRRNLFVVLLDQEGGWYHYHHLFGD
                     FLRRQLELEEEPATIASLNQAASEWFEQQGLIGEAITYATRAGEWQRAATLISGLATK
                     LLVQQREGDLLAWLQSFPPAVISSDPNLSTISAYVLARRGEPGEAGSLLVAAEQRWRA
                     AGSMDGLATVYFVRGAMARFREDATGLMSNAVQAIAEATGEQALDAEGRLAGIRPRLL
                     YLQGTWLGRIGQMLSYAHLAMGLRHLGRVSEAAALYDGIEEAFVASGTALTDTYYTPE
                     AAAIRLAQGRLRQAEQLCRSFLELGTRGLASERMYGCAILGDLLREWNRLDEAEALLR
                     EGITLAERFETPVYLPHLHGALARTLWAAGRTTAALDSLDALAVAARKIQNTHQIHTT
                     AALRVRFALGAGDLEPARQWMATRGSDAAAAPERATVDELLVYARTQMASGEMERVL"
     gene            complement(2647..3135)
                     /locus_tag="VFV93_07670"
     CDS             complement(2647..3135)
                     /locus_tag="VFV93_07670"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX5165256.1"
                     /translation="MATLSVLKFNDPSGADAMLATLQGAQRQELIRIDDAAIVRWPEG
                     AKKPKTQQLSNLTGAGALGGAFWGMLFGLLFFVPVFGMAVGAAFGALGGHFADAGISD
                     DFIKDVKSQVTPGTSALFLMTSGAVVDRIAEMVKGQKFEVLSTNLSPEQEAKLRESFA
                     EG"
     gene            complement(3290..4066)
                     /locus_tag="VFV93_07675"
     CDS             complement(3290..4066)
                     /locus_tag="VFV93_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165257.1"
                     /translation="MGVSDSDLAEQAGTGSPPEHSLRNAGYLTAAAGLIHALLFLLSF
                     WLLAGRPAPDASDAEIVRYYTSDSSRRSILVGLYVMPFAGIAFIWFIVSLRMWISSST
                     RRVNELLSNVQLVSGIIFTTLFFGTAASIAVIAASYEFSGAQVDPDVARQFPQFGASL
                     FFVFGMRMAAMVVFTTTNIGRHAGILPRWFVVIGFAAGLFLLLSATFTVWLAMVFPVW
                     VLVLSLILFLRARQIPRELAVPSSGTLSEGLRSTAVEPGS"
     gene            complement(4299..4814)
                     /locus_tag="VFV93_07680"
     CDS             complement(4299..4814)
                     /locus_tag="VFV93_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165258.1"
                     /translation="MGDGSNGRIGAAIMHRQRFQLCRSRGVSTMRTGPIRVLSVMTLL
                     LLILGACGGDDDPSAEEAKAAYCDDLNALQASIDAFEQTRNDPNATIDDLESARDDIN
                     DKLQDIDDSAEDVADANTEQLDDAYDALNNAVGDIEDDATIGDAAESISDEVQGVRDA
                     WQAVLDTAECS"
     gene            complement(4918..5586)
                     /locus_tag="VFV93_07685"
     CDS             complement(4918..5586)
                     /locus_tag="VFV93_07685"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX5165259.1"
                     /translation="MAQPPWSTSRHASDDPWYAELEQLVQRYEHGEINDEQFAQEKQR
                     ILRRSRAMRARQDQLQIVTAVYDDEQEARAAHDALLQLHIDEVGYIVDAAVLRRDAGG
                     AVGVVQYSERSSGSMAKRSALISVLTELIFPPAGVGGTAGSVAGALVSYLTNRGFDDR
                     DLRALGSSLRPGQSAILVVAQTRRAGQLAGSLHSAASFSSYPLPGEIVTLVNASVDGD
                     ASIS"
     gene            complement(5755..8517)
                     /locus_tag="VFV93_07690"
     CDS             complement(5755..8517)
                     /locus_tag="VFV93_07690"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5165260.1"
                     /translation="MAVPQRIPTPVVQQIPTELLTSRLRPPRARGARVLRPRLVHRVE
                     AASDDPVMMVIAPAGYGKTTLLSQWAASREHAVGWISLSSSENDIAAFFAYLIAALRS
                     VDPALTRIGGNAQLHEQNDLDQLVTNLLNDLAAAGERFALVLDDYHLIESPDVHAIVQ
                     ALIDYAPEPFHLVISSRSEPPFPLARRRVLEQLTTITIDDLRFTLEETIAYCQLAGLK
                     LTDEDVASLRERTGGWAAGIKMVALSIDAATPDAGDFLSRLGTTTQLLQDFLLEEVLE
                     QQPPDVRTALLITSLFDRFNVDLYRAVVDDPAAAQAINAIVRANLFIVSIDANGDWYH
                     YDHLFQAFLRRTAYDEIPDDVRVEAYRRATDWFESRGLIYDAIGLSISSRDWERAADL
                     IRPLADASLKDEEFYPLFMWLRAMPPQLIHSDPAIAYLFAEAAIRAGHDGESETIIAM
                     AEEAAEAGQDLLTLASLADMRAIRARFHDDGAAASHQAERLEQLLRQHDEENPSSVEA
                     DPVGSEQFATGRLARRLRLDLQVLSATTARLLGQTLTAERAAETARLEALLEDKPFCA
                     ATASLELGMALEQQGRLPEASQWFQSSLRLESIYPTDRRMAMLHLADIYRERGQLEDA
                     QQVLEDCLALAHQMEVRTLLAQTRLVFARVAWDRGDEDAAIAYAEEAIAASSEFVSER
                     LRREAHAVVVGIRLTHGDLGAALKWAHESRLAPDDEPDYARLREHLVYARALVAQGEP
                     EDAISLLDRLLASAVADGRNGDVIAILILLATAYQDMVELDKAVAAINRALRLAEPGN
                     YLRVFTTEGQPLARLLKVAQRRGAVSGYCQYVLTAMGEEQQQLTKLYHPELVEPITAR
                     EMEVLRLIAVGLTNREIANELFISVSTVKRHVTNLYGKLGVATRTKALQESRRLGLLT
                     PPVTHG"
BASE COUNT         1753 a         2879 c         2822 g         1703 t
ORIGIN      
        1 aggacccgtt ccatctcacc gctggccatc tgtgtgcggg catacaccag caactcgtcc
       61 acggtggcgc gctcgggtgc agctgccgcg tcgctgccac gggtggccat ccattgacgg
      121 gccggttcca gatcaccagc ccctaacgcg aagcggacgc gtagcgccgc cgtggtgtgg
      181 atctggtgcg tgttctggat cttcctcgcg gcgacagcga gcgcatcgag tgaatccagc
      241 gccgcagttg tccgcccggc tgcccacaac gtccgggcca gcgctccatg caggtgaggt
      301 agatagaccg gcgtctcaaa tcgctctgcc agggtgatcc cctcgcgaag cagcgcctca
      361 gcctcatcca gccggttcca ctcgcgcaac aggtcgccga ggatcgcgca tccatacatc
      421 cgttccgatg caagtccgcg cgtgcccagt tccagaaacg atcgacagag ctgctcggct
      481 tgccggagtc gcccctgagc aagccggatc gccgccgctt cgggcgtgta ataggtgtct
      541 gtcagcgccg tcccgctggc gacaaacgcc tcctcgatgc cgtcatacag cgcagcagcc
      601 tctgacacgc gacccagatg gcgcaggccc attgccaggt gcgcgtaact cagcatctgt
      661 ccaatacgac cgagccaggt accttgcaga tacaggagcc gtggcctgat cccagcgaga
      721 cgcccttcag catcgagtgc ctgctccccg gtggcctcgg caattgcctg caccgcattc
      781 gacatcagtc ccgtagcatc ttcacggaac ctggccatcg ccccccgaac gaagtagaca
      841 gtcgccagcc cgtccatgct gcccgctgcg cgccagcgct gctcggcggc aacgagcagg
      901 gaacccgcct cgccgggctc gccacgccgg gcaagcacat aggcgctgat cgtgctcagg
      961 ttgggatccg aactgataac agcaggtggg aaggactgca gccaggccag cagatcgcct
     1021 tcgcgctgct gcaccagtaa cttcgtcgcc agaccagaga tcagcgttgc cgcccgctgc
     1081 cactcgccgg cccgggttgc gtaggtgatc gcttcgccga tcaaaccctg ctgctcgaac
     1141 cactcgctgg cggcctggtt gagcgaggcg atcgtcgccg gctcttcctc tagttccagt
     1201 tgccggcgga gaaaatcgcc gaagagatga tgatagtggt accaaccacc ctcctggtcg
     1261 agcagcacga cgaacaaatt ccggcgcacg gcgaggtcga gcagttggcc cgcccggcgc
     1321 agccgcgtca ccgttcgaca ggtctgcgcg gtgaaccggt ccaggatcga cgagcgcagc
     1381 aggaactcgc gcatctcgaa atgctgctgg ttcagcacct cctgcaacag gtagtcgccg
     1441 agcatcagca cgtcaccatc aaaggatcgc aacgacctga tcaccgattc cagcgagtat
     1501 tcggttgcat ggatcgcggc cagatggagc gccgcgatcc agccattcgt ctgctcgttc
     1561 agtgccaggc tctcactcgc ggatgcgacg atgccgtagc tgctcagcag ctcgggcagt
     1621 tcagccgacg tgacacgcag atcgttcgcg ttcagccgcg tcagctcctg tcggacgtgc
     1681 agttgtgcca gcggtagcgc tggttcggag cggctcgcga tgatgatccg gcattcgggc
     1741 ggggtaccct cgatcaactg ggtaacgcat tgatcgatct cgttgttggt gatgacatgg
     1801 tagtcgtcga gaatcagaat ccacggctcg gtatgcgccg acagctgact gaggatagcc
     1861 gcgaacaccg acttggcgtc accagggtcg tcgccggtgc cgaggtgtga caccgcatcg
     1921 gcgaggtcgt gatcgacctc gccgatggtt gccagcagat agttcaggaa aacggaaagg
     1981 acattgtcgt cggctccgag ggtaagccat gccactggtt cttcgcgctg tcgcgcccac
     2041 tgtgtgagca aggttgtttt gccataaccg gccggcgaga caatgagcgt gagcctggtg
     2101 acggcggcgt tgagacgctc gatcaagcgt gttcgcgaga tgcgctgggc gcgaagcagc
     2161 ggcggatgga tcctggtttg caccagcaat tggcgtgact cgagtgtcgt cagcatgcca
     2221 gccacctgca cgatcgccgt acaatgtgtc gattgctatt gagcattccc gtatgtgggt
     2281 agtgtactcc gacaacgtcg ccatggcgaa aggtttgccg gcccctggta ccacttacgg
     2341 tacgttagga aaagctgata gtcaatggaa tgctcggtcg cgttggtcag cagcatcgca
     2401 gcgtcaacgt gtctggtggc tcactcgcgt cgttgtcgcg agcatcgtcg cccagagcca
     2461 ttggcagacc agcgccagct caatccagaa gatcgcgtgg gcgtcatact gcatgtttcc
     2521 tccaacaaag aaggaccggc gcaatcgcgc ggccccgcct cgtggtgtcg tactggttgt
     2581 acgtgcgacg gacgaagccg ccgaataccg atccatggca gggatcggta ttcggcgcgg
     2641 ggagttctag ccttccgcga acgactcgcg cagcttggcc tcctgctcag gcgacagatt
     2701 cgtcgacagg acctcgaact tctggccctt gaccatctcg gcaattcgat cgacgacggc
     2761 gccgctcgtc atcaggaaga gcgctgacgt gcccggcgtg acctggctct tgacgtcctt
     2821 gatgaagtcg tcgctgattc cggcatcggc gaagtgcccg cccagggcac cgaatgccgc
     2881 gccgaccgcc atgccgaaga ccggcacgaa gaacagcagg ccaaacagca tgccccagaa
     2941 cgcgccaccc aacgccccag cacccgtcag gttcgacagt tgctgtgtct tgggcttctt
     3001 ggcgccctcc ggccagcgca cgatggcggc gtcatcgatt cgaatcagtt cctgacgttg
     3061 cgccccctgg agggtcgcga gcattgcatc cgctccactc gggtcgttga acttcagaac
     3121 agaaagggtc gccacggttt tctcccttcg aaggttcacg catccacacc gaatgcacag
     3181 acattgctac tggtataggt gccggcgcac tgtccagcat catcctgtgg atggagaggg
     3241 gggtagcaga ttttgacacg agaatcacgg cagcgactca gctgcgcgct cacgatcctg
     3301 gctcgacagc cgtcgaacgc aacccctcgg acagcgttcc gcttgaaggc accgcaagct
     3361 cgcgcggaat ctgccgcgca cgcaggaaga ggatcaggct caggaccagc acccagaccg
     3421 gaaacaccat tgccagccag accgtgaacg tcgcgctcag cagcaggaac agcccggccg
     3481 cgaagccgat caccacgaac cagcgcggca gaataccggc atgccgtccg atgttcgtcg
     3541 ttgtgaaaac gaccatggcc gccatccgca tcccgaacac gaagaacagt gacgctccga
     3601 actgggggaa ctgccgggca acatccggat caacctgcgc gccggaaaac tcgtagctgg
     3661 ccgcgatcac cgcgatcgac gccgccgtac cgaagaagag ggtcgtgaag atgatcccgc
     3721 tgacgagctg cacgttggag aggagctcgt tgacgcgccg ggtgctgctg ctgatccaca
     3781 tccgcaggga aacgatgaac cagatgaacg caatgccggc gaagggcatg acatacaggc
     3841 caacgaggat agaccgccgg ctgctgtcac tggtgtagta acggacgatt tccgcgtcac
     3901 tcgcgtccgg tgcgggccgt ccggcaagca accagaacga gagcagaaac agcaacgcgt
     3961 gaatcaagcc tgcagccgca gtgagatagc ctgcgttacg tagcgagtgc tcaggcgggg
     4021 aaccagttcc tgcctgctcc gcgagatcag agtcggagac gcccatcgtc gtcgcctctc
     4081 aggccgggtg atgccgatct tccggcaaca caccatcgca tgacggagtg ctcaggatcg
     4141 tccaacgccg cgcgggatgt atcagtcagt gggtgtattc agcggtcgta gtcgatgccg
     4201 caaacgggca ccagttggcc ggcgcacggg gcggtgttca gatggcgaac ggctgcaccg
     4261 cagtgcagcc gttcgctgtg atcgcggcaa cctgttgcct acgagcactc tgccgtgtcc
     4321 aggacagcct gccaggcgtc acgcacgccc tggacctcgt cggaaatgct ctccgcggca
     4381 tcgccgatcg tcgcgtcgtc ttcgatgtca ccaacagcgt tgttcagcgc atcgtaggca
     4441 tcgtccaatt gttctgtgtt ggcatcagcg acatcttcgg ctgaatcgtc gatatcctga
     4501 agcttgtcgt tgatatcgtc gcgagcgctc tccagatcat caatcgtggc atttgggtca
     4561 ttgcgcgtct gctcgaaggc gtcaattgaa gcctgcagcg cgttcaagtc gtcgcagtag
     4621 gcggccttgg cctcctcggc agacggatcg tcatcgccac cacatgctcc aaggatcagc
     4681 aacaacagcg tcatcactga caggactcgg attggccccg ttctcatcgt agatactcct
     4741 ctcgaacggc acagttggaa ccgttgccgg tgcatgatgg cagcaccgat gcgtccgttg
     4801 ctgccgtcac ccatcattag ttcttcgggg gcaaggctgc atcatccgcg aggcgtatcg
     4861 gatggttgta gtcgcctcac cccggatggg caatctggat cggccatgct gtcaggatca
     4921 ggaaatggag gcgtcgccgt cgacggaggc gttgacaagc gtcacgattt caccggggag
     4981 tggatagctg gagaagctcg cggcggagtg gagcgaaccg gccaattgcc cggcacgacg
     5041 tgtttgagcg accaccagga tggccgattg ccctggtctg agcgaacttc ccaatgctcg
     5101 caggtcgcga tcgtcaaagc cacgatttgt caggtagctg acgagcgcgc cggccacaga
     5161 cccagcggtc ccgccgacgc ctgccggtgg gaagatcagc tccgtgagca cgctgatcag
     5221 cgcgctgcgc ttcgccatcg agcccgacga tcgctccgag tactgcacga cgcccacagc
     5281 gccaccggca tcacgtcgca gcacggctgc gtcgacgatg tatcccacct cgtcgatatg
     5341 cagctggagc agtgcgtcgt gggcggcgcg tgcctcctgc tcgtcgtcgt agactgccgt
     5401 gacaatctgg agttggtcct gccgcgctcg catagcgcgc gaccgcctga gaatgcgctg
     5461 cttttcctgg gcgaattgtt cgtcgttgat ctcaccgtgc tcgtagcgct gcacgagctg
     5521 ctccagctcc gcataccacg ggtcatccga ggcgtggcga gacgttgacc atggaggctg
     5581 agccatgagc gggtctccaa gtgggcaact gccagcagac aatgattcct gtgatgccaa
     5641 gaatcgggtg tataggaatc cagtagcttc caaccaggcc tacacgatag gtctgagcct
     5701 acgctggctg aggcacaggg gcatcagcca tccagacgca gtcggcgggt gtagtcagcc
     5761 gtgggtgacc ggtggtgtca acaaccccaa ccggcgagac tcctgcaggg ccttcgtccg
     5821 cgtggcgaca cccagcttgc cgtacaggtt cgtcacgtgc cgcttgacgg tcgaaactga
     5881 gatgaacagc tcgttcgcga tctcccggtt cgtcagtcca accgcgatca gccggagcac
     5941 ctccatctcg cgcgccgtaa tcggctcaac gagttctggg tggtacagct tggtgagttg
     6001 ctgttgctcc tcacccatcg cggtcaacac gtactgacag tagccggaaa ccgcgccgcg
     6061 gcgttgcgcg accttcagca gccgcgccag cggctgtcct tcggtcgtga aaacgcgcag
     6121 gtagttcccg ggttcggcca accgcaatgc gcggttgatc gccgcgacgg ccttgtccag
     6181 ttcgaccatg tcctggtacg cggttgcgag caggatcagg atggcgatca catcgccgtt
     6241 gcgaccatcg gcaacggccg acgcaagcag ccggtcgagc aggctgatcg catcctccgg
     6301 ctcgccctgg gcgaccagcg cgcgggcata caccagatgc tcgcgtagcc gggcgtagtc
     6361 cggctcgtca tccggcgcga gacggctctc gtgcgcccat ttgagcgcgg cgccgagatc
     6421 cccgtgtgtg agtctgatcc cgacgacgac tgcgtgggct tcacggcgca gacgctcgct
     6481 cacaaactcg gatgaggcgg ctatcgcctc ttcggcgtag gcaatcgccg catcttcgtc
     6541 accccggtcc caggcgacgc gcgcgaatac gagccgagtc tgagccagca gtgttcgtac
     6601 ttccatctga tgggcaagtg ccaggcagtc ctccagtacc tgctgggcgt cctcaagctg
     6661 gccgcgctcg cggtaaatat ccgcgaggtg gagcatcgcc atccggcggt cggtcggata
     6721 gatcgactcc agccgcagcg aggactggaa ccactggctt gcctccggta gacggccctg
     6781 ctgttccagc gccatcccga gttcgagact ggccgtcgct gcacagaatg gcttgtcctc
     6841 aagcagcgcc tctaaacgcg ccgtctcagc agctcgctct gcggtcaacg tctggcccag
     6901 cagtctcgcg gtcgtcgcac tcagcacctg aagatcgaga cgcaatcgtc tggccagacg
     6961 gccagtagcg aactgctccg agccgaccgg gtcagcttcg acgctggaag ggttttcttc
     7021 atcgtgctgg cgaagcagtt gttccaggcg ttcagcctga tgcgacgcgg ctgccccatc
     7081 atcatggaac cgcgcacgga tggctcgcat gtccgccagt gatgcgagtg tcagcagatc
     7141 ctggccggct tcggcggcct cttcagccat ggcgatgatc gtctcagatt caccgtcatg
     7201 ccctgcccga atggctgctt ccgcgaacag gtaggcgatc gctggatctg agtgaatcaa
     7261 ttgaggcggc atggcgcgca accacatgaa cagcgggtag aactcttcgt ccttcagcga
     7321 cgcatccgcg agtgggcgga tcagatccgc cgcccgctcc cagtcccggc tcgagatgga
     7381 cagtccaatc gcgtcataga tcagaccgcg actctcaaac cagtcagtcg cgcgacggta
     7441 ggcttcgaca cgaacgtcgt cgggaatctc gtcgtacgca gttcggcgca gaaacgcctg
     7501 gaagagatga tcgtagtggt accagtcgcc attggcgtcg atcgaaacga tgaacagatt
     7561 cgcacgtacg atagcgttga ttgcctgggc ggctgcagga tcatcgacga ctgcacgata
     7621 caggtccaca ttgaaccgat cgaagagcga agttatcagc aacgccgtcc ggacatcagg
     7681 tggctgctgt tcgagcacct cttcgagcag gaagtcctgc aggagctggg tcgttgtgcc
     7741 caggcggctg agaaagtcgc cggcatccgg ggtggccgcg tcgatgctga gcgcgaccat
     7801 cttgatacca gccgcccagc cgccagtgcg ttcacgcagg ctggcaacgt cctcgtcggt
     7861 gagcttgagc ccagccaact ggcaataggc gatcgtctct tccagagtga agcgcagatc
     7921 gtcgatcgtg atcgtcgtca actgctcgag cacgcgccgt cgtgcgagtg ggaacggcgg
     7981 ctccgaacgt gaactgatga caaggtgaaa cggctcaggt gcgtagtcaa tcagcgcctg
     8041 gacgatcgca tgaacatcgg gagattcgat caggtgatag tcgtccagga ccagtgcgaa
     8101 tcgctcaccg gcagcggcga ggtcgttgag caggttcgtc acgagctggt cgagatcgtt
     8161 ctgctcgtgg agttgcgcat tgccaccgat acgtgtcagg gctggatcga ccgagcgcag
     8221 ggccgcgatc aggtacgcga agaacgcggc gatatcgttc tcgctcgacg acaacgagat
     8281 ccagccaacc gcgtgttcgc ggctggccgc ccattgcgag aggagggtcg tcttcccgta
     8341 tccagccggc gcgatcacca tcatgaccgg atcatcgctg gcagcttcga cgcggtgaac
     8401 cagccttggc cgaagcacgc gtgcgccgcg cgcccttggt ggacgcaacc tggatgtcag
     8461 cagttccgtt ggaatctgct ggacgaccgg tgttggaatg cgttggggta ccgccatcgg
     8521 tatccctccc ctacgacgtc gacggccgga aatcttgacc atagccactt acgcggcaag
     8581 cggtgaatgg atggattcta gacatctcca ctcgcaatgt ctagcccttt tgcggttgat
     8641 ttcggaagtt tgatgagccg cgtcttcatg acgcagcgac aacccccctg aattccctgc
     8701 cgaaaccggc aagaccttga gacattgttt gcgcggcatt ggcagtctca acgcgcggtg
     8761 tcggttgctg attggttggc ggctgtcgcg agggcgtcga tcccgccgcg ccgccaggga
     8821 aagcaccgtt cgagccgtga tcgtctgtgt gccgcccgcc ggggaccagg aacaccgctc
     8881 tccgtggtca agcgccccgt tcgctggcgg gctgattgat cccccggttt ccacccacca
     8941 ctacgcccaa ccggtggatg ttgggcggga gagaggccag tactgtcgat cgtgcggaca
     9001 caggatcaac accgcagctg tctcgatggg gcggcatgga tctcgacacg ttgagaagcg
     9061 aagtggagtg acctgatgac gggtctcgaa tggttgctgt cgtggacgct gttcatgatc
     9121 tacatcacct gcctgttcac cgtctgccgt ctgacct
//
LOCUS       DASUGJ010000346         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1027085, whole genome shotgun sequence.
ACCESSION   DASUGJ010000346 DASUGJ010000000
VERSION     DASUGJ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027085"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1054)
                     /locus_tag="VFV93_07695"
     CDS             complement(92..1054)
                     /locus_tag="VFV93_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165261.1"
                     /translation="MAQRTKVSKLNPVATAREIFSIIREMSLDDLRDAALRQPRVAVV
                     ARSVGEARLVAADVFGPEAPDLVVALGEHDDWPYAAEIVLLERRARPDRSHDRYGLIV
                     EFSADEPADRLRQAVVRAGDDLELALGRAFPALRQAAAMQVVNVTSRVNGQFALASNL
                     PALVPVVGGVLAAGADTIVLTKNQLMMLYKLAAIHDRDLDNRFRIYQEMIPVVGAGLF
                     WRTVARDLAAMMPFAAGAVPKVAIAFAGTFAAGMAAHLYYQEGKRVDKQRMRQYYRDA
                     LQQLRDRPDLLKSLPIPNRLRRDDDANGVIVDVDYTSDASPEMP"
     gene            complement(1125..2108)
                     /locus_tag="VFV93_07700"
     CDS             complement(1125..2108)
                     /locus_tag="VFV93_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX5165262.1"
                     /translation="MARLPETTRIARVLDIVWHINAAPRYWTRKRLAEKFEVSERSIT
                     SDLDLIRHRLKFDLENDRGNGYYFAKVPQLPSVSYSIAEALALILAARAARQFPGVPQ
                     RDLSLAITRLSSVIPGELRAMVEQVANDGLGESDAHREEVLSRCSQAISASHSLEIVY
                     AAASANGEETRRTIDPYAVFPYDRSWHVVGYCHLRDDVRVFKVDRIRAASQTGAAFER
                     PADFDLEGFLSTGWGLMRGLELPVEEVVLRFRPPASRWVAEERWHESQQVIPLADGSV
                     EFRVTIQVTPEFQRWVFRFGSTVDVIAPASLREWMACEARAVLTSVQATDA"
     gene            complement(2120..>2683)
                     /locus_tag="VFV93_07705"
     CDS             complement(2120..>2683)
                     /locus_tag="VFV93_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165263.1"
                     /translation="RMLRRGAIVVSSDRTVEAPVIAAGLQTGRRSGARRARIEIRPDL
                     ADLMTSDAGWGRVAGTIAPAVRLAPGHVPLTGGQRFEVDIIARDAFSDDPLVVRAGIR
                     NGQIVHSVAHWWQNVQPDTTEVGPRPLSTVPAFADLGRQYPDAHYGGFAAGSALLGCL
                     LAGLDVALDRCDRPLPPSSANALVSGD"
BASE COUNT          480 a          928 c          869 g          406 t
ORIGIN      
        1 gcgaggcgct gcggcaggcc aacggcgacc gcgtgacagc cgcgtcgctg cttggctacg
       61 acatgaccga gttgagcgag aagctgagcg actacggcat ctccggcgac gcgtcagagg
      121 tgtagtcgac gtcgacgatg accccgttag cgtcatcatc gcgccgcagg cggttcggga
      181 tcggcaggga cttgagcagg tccggccggt cccgcaactg ctggagcgcg tcccggtagt
      241 actgccgcat ccgctgcttg tcgacccgct tgccctcctg gtaatacaaa tgcgccgcca
      301 tcccggccgc gaacgtgccg gcgaaggcga tcgccacttt cgggaccgcg ccggccgcga
      361 acggcatcat cgccgccagg tcgcgggcaa ccgtccgcca gaacagcccg gcgccgacga
      421 ccgggatcat ctcctggtaa attcggaagc gattgtcgag gtcgcggtcg tggatcgccg
      481 ccagcttgta gagcatcatc agctgattct tggtcaggac gatcgtgtct gctccggccg
      541 cgagcacccc gccaacgacc ggaacgagcg ccggcaggtt cgacgccagc gcgaactgcc
      601 cgttgacgcg cgaggtgacg ttgacgacct gcatcgccgc cgcctgccgc agcgcgggaa
      661 acgcgcggcc cagcgccagc tccagatcgt cacccgcgcg gacgaccgcc tgtcgcaacc
      721 gatcggccgg ctcatcggct gagaactcga cgatgagacc atagcggtca tggctacgat
      781 cggggcgggc acgacgttcg agcaggacga tttcggcggc gtagggccaa tcgtcgtgct
      841 cgcccagcgc aacgaccaga tccggcgctt ccggcccgaa gacgtctgct gcgaccagcc
      901 gcgcctcgcc aacgctgcgc gcgacaacgg ctacacgcgg ctggcgcagc gcggcgtccc
      961 gcaggtcgtc gagcgacatc tcgcggatga ttgagaagat ctcacgtgca gttgcaactg
     1021 gattgagctt cgaaaccttg gttcgctggg ccactcgtta ccgttctcct cgcgaaagcg
     1081 cgtctccaac gatcatgcac gttcgcacgg cccgcgccaa cctgctacgc gtccgtcgcc
     1141 tgcaccgacg tgaggacagc acgcgcctcg caggccatcc actcccgcaa cgatgccggc
     1201 gcaatcacat cgacggtact tccgaagcgg aacacccagc gctgaaactc cggtgtcacc
     1261 tgaatcgtga cccgaaactc aaccgatcca tcagcgagtg gaatcacctg ctggctctcg
     1321 tgccagcgct cctcagcgac ccagcgcgag gcgggcggcc gaaagcgcag gacgacctct
     1381 tcgacgggca gctccagccc gcgcatcagc ccccaaccgg tgctgaggaa cccttccagg
     1441 tcgaagtcgg ccggacgctc gaatgcggcg ccggtctgcg acgctgcgcg gattcgatcg
     1501 accttgaaga cgcgaacgtc gtcacgtaga tgacagtagc cgacgacgtg ccaggagcgg
     1561 tcgtacggga agacagcata ggggtcgatc gtccgccgcg tctcctcgcc gttggcggac
     1621 gccgccgcat agacgatttc gagcgagtgg gatgccgaga tcgcctggct gcaccgcgac
     1681 aacacctcct cgcggtgcgc gtcactctcg cccagcccgt cgttggcgac ctgctcgacc
     1741 atcgcccgca actcaccggg aatcaccgaa gaaagtcgcg tgatcgccag cgagaggtcc
     1801 cgttgcggca caccggggaa ctggcgcgcc gcccgcgccg cgaggatcaa tgccagcgcc
     1861 tcggcaatcg agtacgagac ggacggcaac tgcggaacct ttgcgaagta gtagccgttg
     1921 cctcgatcgt tttccagatc gaacttcaga cggtgccgga tcagatccag gtcggacgtg
     1981 atcgagcgct ccgagacctc gaacttctct gccagccgct tgcgcgtcca gtagcgcggg
     2041 gcggcgttga tgtgccagac gatatcgagc acccttgcga tcctggtcgt ctctggtagc
     2101 cgtgccatct cagcctcctt caatcgccac tgacgagcgc gtttgcgctg gaaggaggca
     2161 gcggccggtc gcagcggtcg agtgccacgt cgagaccggc cagcaggcaa ccgagcaggg
     2221 ccgacccggc cgcgaagccg ccgtaatgcg cgtctgggta ctgccggcca aggtccgcga
     2281 acgccggcac ggtcgagagt ggacgcggcc cgacttcggt cgtgtcaggc tggacgttct
     2341 gccaccagtg tgcaaccgag tggacgatct ggccgttgcg gatgccggca cgaacgacca
     2401 gcggatcgtc gctgaaggca tcgcgagcga tgatatcgac ctcgaaacgc tggccgccgg
     2461 tcagcggcac atgccccggt gcgagccgga cggcgggagc gatcgtaccg gcaacgcgtc
     2521 cccagccggc atccgacgtc atcagatccg ccaggtcggg cctgatctcg atccgcgccc
     2581 ggcgggcacc gctgcggcgc ccggtctgca ggccggcagc gatgaccggc gcctcgaccg
     2641 tccgatcgct gctgacgacg atggcgccac gccgcagcat tcg
//
LOCUS       DASUGJ010000347         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1721656, whole genome shotgun sequence.
ACCESSION   DASUGJ010000347 DASUGJ010000000
VERSION     DASUGJ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721656"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFV93_07710"
     CDS             <1..760
                     /locus_tag="VFV93_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5165264.1"
                     /translation="DLSTLRTGIMAGSPCPIEVMREVNSRMHMDEVTICYGMTETSPV
                     SFQSTTDDPLEKRVSTVGRIHPHVECKIVDPDTGAVVPRGMTGELLSRGYIVMLGYWN
                     NQQATAEAIDDGRWMHTGDLAVMDEEGYVNIVGRAKDMIIRGGENIYPREIEEFLYQH
                     PKIRDVQVIGVPDEQYGEQVMAWVVLRPGQGATVEELTEYCTGQIAYYKVPKFWRFCD
                     SFPMTVTGKVQKYKMREMAIEELRLEQAASIRTA"
     gene            768..1367
                     /locus_tag="VFV93_07715"
     CDS             768..1367
                     /locus_tag="VFV93_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165265.1"
                     /translation="MKRGWSERWPWRRDSVEIQDDESGDDESPEAPSVDVVFAEMRRT
                     LDAQQAQITMLDTKAAFILTSASIIVGFSGLSGLGPDQRSGSSGWLIPALLVYLALIV
                     SVIQAYRLRGFSSSGLEPETLPDYLFERPEVTKRQMIATMREIHRANVEPVNTKIRWL
                     RRAEILLATEAAYFVLVIIGQSQWNEIRNIADRAASSLT"
     gene            complement(1616..1993)
                     /locus_tag="VFV93_07720"
     CDS             complement(1616..1993)
                     /locus_tag="VFV93_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165266.1"
                     /translation="MGHPACLAELRELQCIAHRAHDRFDLSTSFRISVDRLAKLAKAV
                     RRTKVASEFDTIGDSVAILPHADQAFFVHDPEQFGSRHRRGATIRYATIALWFFALRA
                     LHDLSTSPRNAAKQALVPEAAMS"
     gene            1985..2170
                     /locus_tag="VFV93_07725"
     CDS             1985..2170
                     /locus_tag="VFV93_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165267.1"
                     /translation="MTHREVAETLEVSQANISRIEHEDDLYLSTLSGYVAALGGRLEL
                     RAVFPDQTVEIVPVCKP"
BASE COUNT          479 a          702 c          730 g          402 t
ORIGIN      
        1 cgatctctcg acgctacgga ccggcatcat ggccggctcg ccctgcccga tcgaggtcat
       61 gcgcgaggtc aacagccgga tgcacatgga cgaggtcacg atctgctacg ggatgacgga
      121 gacctcgccg gtctcgttcc agtcaacgac cgacgacccg ctcgagaagc gcgtcagcac
      181 ggtcgggcgg attcacccgc acgtcgagtg caagatcgtc gatccggaca ccggcgccgt
      241 cgtcccacgc gggatgacgg gtgagctgct ctcacgcggc tacatcgtga tgctgggcta
      301 ctggaacaac cagcaggcca ccgccgaggc gatcgacgac ggccgctgga tgcacaccgg
      361 cgatctggcc gtcatggacg aggaggggta cgtcaacatc gtcggccgcg ccaaggacat
      421 gatcatccgc ggcggcgaga acatctaccc gcgcgagatc gaggagtttc tctaccagca
      481 ccccaagatc cgcgatgtcc aggtgatcgg cgtccccgac gagcagtacg gcgagcaggt
      541 gatggcctgg gtcgtcctgc gccccggcca gggcgcgacc gtcgaggagc tgaccgagta
      601 ctgcaccggc cagatcgcct actacaaggt gcccaagttc tggcgcttct gcgacagctt
      661 cccgatgacc gtcaccggca aggtccagaa gtacaagatg cgcgagatgg ccatcgaaga
      721 gctccgactg gaacaggcgg ccagcatccg gacggcgtga gcgagtgttg aagcgtggat
      781 ggagtgagcg ctggccgtgg cgtcgagact cagtcgagat tcaagatgac gagtccgggg
      841 atgacgagtc gccggaggcg ccgagtgtcg atgtcgtgtt tgcggagatg cgacgcacgc
      901 tcgacgcaca gcaggcacaa atcacgatgc tcgatacgaa ggccgccttt atcttgacct
      961 cggccagcat catcgtcggc ttttctggac tgtctggact cggtccagat cagcgttcag
     1021 ggagttcggg atggctgatt cccgcgctgc tcgtctatct cgcactgatc gtctcggtta
     1081 tacaggcgta ccggctccga ggcttcagca gtagcggact ggagccggag acgttaccgg
     1141 attacctgtt tgaacggccg gaagtcacga agcggcagat gatcgcaacg atgcgcgaaa
     1201 ttcacagagc gaatgtcgag ccagtcaaca cgaagattcg gtggctgcgg cgcgcggaga
     1261 tcctgcttgc taccgaggca gcctactttg tccttgttat cattggacag agtcagtgga
     1321 acgagattcg caacatcgcc gatcgagcag ccagcagtct gacgtaactg attcatcgaa
     1381 agtgagacca gatgtcatcc gtcacggtac gcaatcccaa actcccgcag gatccggacg
     1441 cgccaaatcc gaagttgatg aagcctgctg agtacaagga agcaccgccc cggcggccgg
     1501 ccaccacgaa ggcgagcacc caccctcgcc ttcgcgacga ccatgcggtt cgtcactcca
     1561 atccatcata gtcagccgta ccccacgttc aaatcaccaa gatcatgtcg cgttcctacg
     1621 acatcgccgc ttctggcacc aatgcttgtt tcgcagcgtt tcgtggtgaa gtggagagat
     1681 catgcaacgc gcgcaaagcg aagaaccaga gcgcgatcgt cgcgtagcgg atggtcgccc
     1741 cgcgacgatg acggctgccg aattgttcag gatcatggac gaagaaggct tgatcggcat
     1801 gtggaaggat cgcgacacta tcgccgatag tgtcgaattc gctcgcaacc ttcgtacggc
     1861 gcacagcttt cgcaagcttc gcgagccgat cgacgctgat ccgaaacgac gtgctgaggt
     1921 cgaagcgatc gtgcgcgcga tgcgcgatgc attggagctc acgcaactcc gcgaggcacg
     1981 cgggatgacc catcgcgagg tcgccgagac actggaggtc tcgcaggcca atatctcgcg
     2041 gatcgagcat gaggacgatc tgtatctctc aacgttgagc ggctacgtgg ctgccctcgg
     2101 tggccggctg gagttgcgcg ccgtcttccc cgatcagacc gttgagatcg tcccggtgtg
     2161 caagccgtag cgccgatcgg gaccggaacg tctacgttgc tcgcgccagg gcatagcgca
     2221 ggaagaggtg gctgcctgcc cgccggagat cgatgagtgt gccccagagc gggtcgtccg
     2281 gcgcgaagag gtggccctcg accagcccga gcc
//
LOCUS       DASUGJ010000348         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1303836, whole genome shotgun sequence.
ACCESSION   DASUGJ010000348 DASUGJ010000000
VERSION     DASUGJ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303836"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFV93_07730"
     CDS             complement(<1..341)
                     /locus_tag="VFV93_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165268.1"
                     /translation="MTHPHAAARDRLYAAIDARQDELVDLVQQLVRCRSVLGNETGAQ
                     RLVADYIRAIGVEPDVWEMDNSLLERPGSGNSGVSFEGRPNVAAVYAGSGGGKSLILN
                     GHIDVVSPEPL"
     gene            506..1096
                     /locus_tag="VFV93_07735"
     CDS             506..1096
                     /locus_tag="VFV93_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5165269.1"
                     /translation="MEFAEVVRKRRMVRHFKPDPIDPEIVDQMLRLAQRAPSAGYTQG
                     QSFIIVTDPEMRKAVARCCGEERYASLSRHPWISEAPVQAIPCVSEAAYHRRYQEQDK
                     INDDGSEIEWPVPFWFLDIGCSVMALLLAAVDAGLAAGYAGIPDTAALKELLGIPDEV
                     TPVGVIPIGYADQDIPSPSLKRGRTAFEGFVHRERW"
     gene            1118..1609
                     /locus_tag="VFV93_07740"
     CDS             1118..1609
                     /locus_tag="VFV93_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165270.1"
                     /translation="MIADWDIPAAKPPASDAATQVLVVVPLPGEMGGAGEVLVLSAFG
                     NLKLPAGELQPRETSLRAASRIALGVAGVRVAPERLVYVIEQPGKPLALAILCGLIAD
                     DDADTKPGIRFADPGASAGDFEPAPLRELLIEDVRAGFVRGVAHITIGYDDTGREQVS
                     TTW"
     gene            1653..>2524
                     /locus_tag="VFV93_07745"
     CDS             1653..>2524
                     /locus_tag="VFV93_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165271.1"
                     /translation="MTQRWKRGQTFIAILCAMLLVVTAACGSGDDDDNESEATTAATS
                     TATKAAAAADTSPTKAETAGATATTAAETATATETEDTEPTATTRAEATATTPASDEA
                     TATSTATEPAGATSTAGVGDIEVPDFETLDPELLPNFTLTMNMTSTNMDDVPESTMKM
                     EMAQSDVDTYHVSVDTDGDVIEFWAVDGRYWTSIGGEIVESPTGSIFSPSDILTTGEL
                     IPDDLEAREDGHEEVNGRQTTRWVIDGDKYVEYMNQQNVASGVSTGEMTDGSGEVTIW
                     IDDELKIMIKANSDV"
BASE COUNT          502 a          821 c          816 g          385 t
ORIGIN      
        1 tcgagcggtt cgggactgac gacatcgatg tggccgttga ggatcagcga cttcccgccg
       61 cccgaaccag catagacggc cgcgacgttt gggcgaccct cgaatgacac gccactgtta
      121 ccggagccgg ggcgctccag cagcgagttg tccatttccc agacatccgg ttcgacgccg
      181 atcgcgcgaa tgtagtcagc gacgaggcgc tgagcgccgg tctcgttgcc aagcactgat
      241 cggcaacgca cgagttgctg cacgagatca accaactcgt cctgccgtgc gtcaatcgcc
      301 gcgtagagcc gatcgcgagc agccgcgtgg ggatgtgtca ccagcctcct ccaactcgca
      361 tgagttcgca tgcctgcagc ccgcagcata gcagtcttca cgcccggcgg ccttgtcgct
      421 accgtgcccg gcagggtatc gtcgcgcctg cccgtacgtg cagccgccac gtacgctgac
      481 gagctggcag agaaatggag tcgtcatgga attcgccgag gtcgttcgca agcgccggat
      541 ggtgcggcac ttcaaaccgg acccgatcga cccggagatt gtcgatcaga tgctgcggct
      601 ggcccagcgc gcgccaagcg ccggctacac gcagggacag tcgttcatca ttgtgaccga
      661 ccctgagatg cgcaaggcgg tcgcgcgttg ttgcggcgaa gaacgctacg cctcgctctc
      721 ccgtcatccg tggatctcgg aggcgccggt ccaggcgatc ccctgcgtca gcgaggccgc
      781 ctaccaccgg cgctaccagg agcaggacaa gatcaacgac gacggcagcg agatcgaatg
      841 gccggtcccg ttctggtttc tcgatatcgg ctgttcagtc atggcgctgc tgctggccgc
      901 cgtcgatgct ggccttgccg ccggctacgc gggtattccc gacaccgcgg cgctgaagga
      961 gctgctcggc atccccgacg aggtgacgcc ggtcggcgtg atcccgatcg gctatgccga
     1021 ccaggacatc ccatcgccgt cgctcaagcg cgggcgaacc gcgttcgagg gctttgtcca
     1081 ccgcgagcgc tggtaacgca tccccaagga ggccgccatg atcgctgact gggatatccc
     1141 ggccgccaaa cctccggcat ccgacgcggc gacacaggtg ttggtcgtcg tgccgctgcc
     1201 gggcgagatg ggcggggcgg gcgaggtact ggtcctctcg gcgttcggga acctgaagct
     1261 cccggccggc gagttgcagc ccagggaaac aagcctacgg gccgcctcac ggatcgcgct
     1321 cggcgtggcc ggtgtccgtg tcgctcccga gcggctggtc tacgtcatcg agcagcccgg
     1381 caagccgctt gcgctcgcca tcctgtgcgg gctgattgcg gacgacgacg cagacaccaa
     1441 acccggcatc cgctttgccg atccgggagc gtcggccggt gatttcgagc cggcgccgct
     1501 gcgcgagctg ctgatcgaag acgtccgtgc cggcttcgtg cgtggagtgg cacacatcac
     1561 gattgggtat gacgacactg gacgagagca agtatcgacg acctggtaat gcacgacaat
     1621 tggatcatcc ccgaaatgag aggacgaaac tggtgacaca acgctggaag cgtggacaga
     1681 cattcatcgc catcctctgc gcgatgctgc tggtcgtgac ggccgcctgc ggtagcggcg
     1741 acgatgacga caacgaaagc gaagcgacga cggccgccac ctcgacggcg acgaaggcgg
     1801 cagccgcagc cgacacctcg ccaaccaagg ccgagacagc cggcgcgacg gcaacgaccg
     1861 ccgcggagac cgcgacggcg accgagaccg aggacaccga gccgaccgcg acaacacgcg
     1921 cagaggcgac cgccacgacg ccagctagcg acgaggcgac cgccacctcg acggcaaccg
     1981 aaccggcagg ggcaacctca acagcgggcg ttggcgatat cgaagtgccg gacttcgaga
     2041 cgctcgatcc ggaattgctg ccgaacttca cgctgacgat gaacatgacc tccaccaaca
     2101 tggacgacgt ccccgagtcg acgatgaaga tggagatggc ccagagcgat gtcgacacgt
     2161 atcacgtcag cgtcgacacc gacggggacg tgatcgaatt ctgggccgtc gatggcagat
     2221 actggacgtc gatcggcggc gagatcgttg agtcaccaac cggctcgatc ttcagcccat
     2281 ccgatatcct gacaaccggc gaactgatcc ccgacgatct cgaagcccgc gaggacggcc
     2341 acgaagaggt caacggccgc cagacgacgc gctgggtcat cgacggcgac aagtacgttg
     2401 agtacatgaa ccagcagaac gtcgccagtg gtgtctcaac cggtgagatg acggatggat
     2461 ccggtgaggt cacgatctgg atcgacgacg agctgaagat catgatcaag gccaacagcg
     2521 acgt
//
LOCUS       DASUGJ010000349         9300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_53767, whole genome shotgun sequence.
ACCESSION   DASUGJ010000349 DASUGJ010000000
VERSION     DASUGJ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53767"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..448
                     /locus_tag="VFV93_07750"
     CDS             131..448
                     /locus_tag="VFV93_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX5165272.1"
                     /translation="MPTYEYACTNCGHRFDVRQSFHDEPISDCPECGASVRRVLYPAG
                     VIFKGSGWYATDNRKGSASHSSSTSPSSDTSSTSSTETKAAPDKSEAPAKDAKAKTAA
                     ADD"
     gene            586..1971
                     /gene="cysS"
                     /locus_tag="VFV93_07755"
     CDS             586..1971
                     /gene="cysS"
                     /locus_tag="VFV93_07755"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5165273.1"
                     /translation="MSLTVYNSMSRTKEPFETIEPGVVRMYVCGVTVYSDAHIGHARA
                     FITFDVIRRYLEWIGFTVHHAQNFTDVDDKIINRARQLGISEEELTNRLIEDWAEETR
                     ALNIMPATVYPRATQEIPEIIAMIEGLIRLEHAYPVEGGDVNYRVSSFPEYGKLSGRK
                     LDDMQAGARIEVDPRKEHPMDFALWKAAKPGEIAWDSPWGPGRPGWHIECSVMALQHL
                     GHHLDIHGGGEDLIFPHHENEIAQSEAFPENRPFARYWMHNGMLQFSGDKMSKSIGNL
                     INIRELVANGDARAFRLMALQTIYRAPLNYTDEGLEAARRGLERLDLSVRGFDGTGAA
                     ASSAVDGELADAERRFREAMDDDFNTPVAVSVLFDLSRAANRSEGVDKAAAQAKLIEL
                     AGVLGLELRAPEAGGAGDAGPFIDLLLEIRAALRAEKNWTLSDAIRDGLAERGVTVED
                     SPTGATWRRRD"
     gene            1928..2833
                     /gene="rlmB"
                     /locus_tag="VFV93_07760"
     CDS             1928..2833
                     /gene="rlmB"
                     /locus_tag="VFV93_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX5165274.1"
                     /translation="MRTRRPGQPGAGGTKRNSGGRPERSGKPGRSGSGRPATEHGGGR
                     RQPERRDRPPRRDDGNDLLYGRNAVREALRAGRRRFIRLLVNEGAADSQRLTEIVTLA
                     RQRDVGIETVSRDTLDAKTPGQHQGVVLEASTYPYAVGDELRELAEDQAILLALDGIA
                     DPQNLGTLLRTAEATGVRLIVIPSDRAAQVTPAVVNASAGAVEHLHVAQEVNLTRWLG
                     RAKDAGFWIVGMDGEDAEPLFDTSMRPPVVLVVGSEGSGIRRLVRETCDIIVALPMLG
                     RIESLNAAVAGSIGLYEIVRDAEED"
     gene            2970..3542
                     /gene="rplI"
                     /locus_tag="VFV93_07765"
     CDS             2970..3542
                     /gene="rplI"
                     /locus_tag="VFV93_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX5165275.1"
                     /translation="MKIILTQDVPKLGSSGTVQDVKPGFARNYLIPQGMAVIATRGSI
                     KQVEERQAADARRIAKQEEELRGLSERLQGMRVQIEARVGEQGRLFGSVTAADIAEKV
                     SAQVGEEIDRRKIDLDEPIRTVGEHAVTIRLVGRLQPTINVVVWDPENPHASVTDEPA
                     AEAAAEGDEDVDGDAVAVVAAEDENDTETE"
     gene            3549..4889
                     /gene="dnaB"
                     /locus_tag="VFV93_07770"
     CDS             3549..4889
                     /gene="dnaB"
                     /locus_tag="VFV93_07770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX5165276.1"
                     /translation="MMQAVDRLPPHNLEAEQSVLGSLLIDRDAIIRIASYVKSSDFYR
                     AAHGLIYEAIVTLYNKREPTDLITLVDELRRTNRLEDAGGESYLSELIAAVPTAVHVE
                     YYGRIVERTATLRRLISAGTEIVDIGYTESMDIEQALDGAEKAIFAVSQKRSTRDFRS
                     IGEILEEYFDRLDTLQQNKGAVVGVPTGFIDLDKLTGGLQRSDLIIIAARPAVGKSSL
                     GLGFAFNAAVRFQKTVGLFVLEMSAEQIVQRMLSMETGIDSHRLRMGYIDDSEWSSVT
                     RAFGRLAEANIFIDDTASANIMEVRSKARRLHAERGLDLLIIDYLQLMTASRADNRVQ
                     EISQISRALKGLARELDVPVVALAQLSRAVETRQSHVPMLSDLRESGSIEQDADIVAF
                     IYREDVYDPDTDRKGIADLIIAKHRNGPIGTVHLRFFDRTARFADLELYREPDL"
     gene            4932..5630
                     /locus_tag="VFV93_07775"
     CDS             4932..5630
                     /locus_tag="VFV93_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEX5165277.1"
                     /translation="MPDGDSAVPDDLIPAAVLSRLVREVWSPLEVKLVLAVAVLGGLH
                     DPVREESLLIDEGLAAGMRADGSSRGTVERLLETIELATARGVLLRLVDDDGDYWLIL
                     GVDANRRKVRSGLVFAGETAQRWQGTLRVERPSIFTLYEQNVGLVTPIIADRLVEAIE
                     RYPEAWVEDAIEEAVAYNRRNWRYIQRILENWASEGRTDEANRGDPARDLNRDKHLRG
                     KYAHLFRRDNLPDL"
     gene            5650..6327
                     /locus_tag="VFV93_07780"
     CDS             5650..6327
                     /locus_tag="VFV93_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5165278.1"
                     /translation="MDASVTSANFSRLVPCTCKVEERRQRSQNNLRVFSDLSPFRDQT
                     FDSFDPGVRGTRDAFEAAAGYARHPDGWLVLVGDYGCGKTHLAAAIANFALTEREMGP
                     IVAVVPDLLDYLRTTFSPDSDVSYDDRFNAFRGADLLILDDLGTENATPWAREKLFQI
                     INHRYNERSPLVVTTNDLDRVDPRIRSRLCDWQLSRHVYIDADDYRDRSGSRRRLRTN
                     GNGQKRS"
     gene            6418..8610
                     /locus_tag="VFV93_07785"
     CDS             6418..8610
                     /locus_tag="VFV93_07785"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5165279.1"
                     /translation="MSGARMTKDNDLSLTQSDFRPQGEMYLMPDPNQPPAEVISFNRT
                     PAQSSDPNESFELRDMVSLAITYFDRGMIDDAEEMLNEALEAGYSRQDAVDLLRRVQS
                     VRGQTTMVGAARTAHSEAEQQATDSVVHEFTMPLPGVDSQTSLVRRAIEDADRDLAAG
                     RLESAHDATLHALAMAPSYLPIYVRLAEIRTAFGDSESAETLIGSLKAVLESIGDERD
                     WLTQSMRVTLDPDNTDALVQLARSLVEQKGTLQLEPYVPEAIERTLADNPEVALELAR
                     EYIRLRPNMRQAQRLHLRAVVAEGDIAQVTSLLEREVTPDAPADLLFLRSCVAHSEGR
                     ETWFHWLERAVSVVLGGDYDQDDLARAVDASRNLLPNPQHALATSVVRMAGNDPHGAL
                     AVLDPWSGIPGRETSNASEMLIAACARAFSLRAISPIESIEALSNAVGQAVVLDVRPF
                     AEACRLFARSISAEALMHELVAVVRETGQHEMAIRQLQALRDRMPEHLEIRTGLADLQ
                     VAAGRTAEGVRELRYIAERYEQAGNIDRMVDAMRHISAAVPNNVEMKAKLIEGYVQRG
                     VPEDAMRELRLLGDLHHKRGRMAEAAAAYTRGAEIASTTGNYRRAMDLFERAIGADPE
                     NVGVRHAAVAYYIMVGSVDKATEQLREVVRIALQEQDPDEAVAALHQIIGLSPAEASA
                     YHKLGEVLTSLGEYAQAERVYRRLAAFTPDDPVLTAKQSALAALAAGQ"
     gene            8704..>9300
                     /locus_tag="VFV93_07790"
     CDS             8704..>9300
                     /locus_tag="VFV93_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEX5165280.1"
                     /translation="MPELPWIFTRITARALVDISAVALIIYWLLWFAQRTRAAQLIRG
                     LIILIVGVLLASNIFDLTALNWLFSRAWPAMIVAIPIIFQPELRRALEQLGHTGSWIP
                     KPFGQPVDADVDRTIDEISRAAAHLSRIGYGALLVIERETGLQDYADRGVTLDAVVSR
                     QLLINIFFPNSPLHDGAVIVRGNRLLAANCILPLSDREI"
BASE COUNT         1753 a         3026 c         2947 g         1574 t
ORIGIN      
        1 atgcgcgact tctagcggca gcacaatttc aatggtcaat ccggccgggt acccgcaaaa
       61 cgcgcgcaat cggctatact tgggtgttcg tttcggacgg cgagacgata gaaggagttc
      121 cacccgggcc atgccaacct acgaatacgc ttgcacgaac tgtggccacc gtttcgatgt
      181 gcgccaaagc tttcatgatg agccgatcag cgactgtccc gagtgtggcg ccagcgttcg
      241 ccgcgtgctg tatccggccg gagtgatttt caagggttcc ggctggtacg cgacggacaa
      301 ccgcaaaggt tcggcgtcac actccagcag cacatcacca tcatcagata cctcctcgac
      361 ctccagcacg gagacgaagg cggcgccgga caagtccgag gcaccagcca aggacgccaa
      421 ggccaagacc gccgctgcgg acgactagcc agcacatccg gcggtcgttc ggatcgccgc
      481 agcaaccggt tggagacaac gacgggggat ctgctgaccg cttcccccgt cgattcgttg
      541 tgtcgcgagc cggtgtcgct gccggcgaga agggctgctc gaaccatgtc gctgactgtt
      601 tacaactcga tgtcccgcac caaggaaccg ttcgagacga tcgaacccgg cgtcgtccgc
      661 atgtacgtct gcggcgtgac cgtctactcc gacgcgcaca tcggccacgc gcgggccttc
      721 atcaccttcg acgtcatccg ccgctacctg gagtggatcg gcttcaccgt ccaccacgcc
      781 cagaacttca ccgacgtcga cgacaagatc atcaaccgcg cccgccagct tggcatcagc
      841 gaagaagagc tgaccaaccg gctcatcgag gactgggccg aggagacgcg tgcgctgaac
      901 atcatgccgg ccaccgtcta cccgcgcgcg acgcaggaga tccccgagat catcgcgatg
      961 atcgaagggc tgatccggct ggagcacgcc tatccggtcg agggcggtga cgtgaactac
     1021 cgcgtctcca gcttcccaga gtacgggaag ctctccggcc gcaagctgga cgatatgcag
     1081 gccggcgccc ggatcgaggt cgatccgcgc aaagagcatc cgatggactt cgccctctgg
     1141 aaggccgcca aaccgggcga gatcgcctgg gacagcccgt ggggccccgg caggcccggc
     1201 tggcacatcg agtgctcagt gatggcgctc caacatctcg gccaccatct cgacatccac
     1261 ggcggcggcg aggacctgat cttcccacac cacgaaaacg agatcgccca atcggaagcg
     1321 tttccggaga accggccgtt cgcccgctac tggatgcaca acggcatgct gcaattctcc
     1381 ggtgacaaga tgagcaagtc gatcggaaac ctgattaaca tccgtgaact ggtcgccaac
     1441 ggagacgccc gggcattccg actgatggcg ctgcaaacga tctaccgcgc gccactgaac
     1501 tacactgacg aggggctcga agcggcccgg cgcggcctgg agcggctcga cctgtccgtt
     1561 cgcggctttg atggcaccgg tgctgcagca tcgagcgcgg tcgatgggga actggccgac
     1621 gccgaacgac gcttccgtga ggcgatggac gacgacttca acacaccggt tgccgtctcg
     1681 gtcctcttcg acctgtcgcg ggccgcgaac cgatctgaag gtgtcgataa ggcagctgcc
     1741 caggcgaagc tgatcgagct tgccggagtc ctcggactcg agctgcgcgc gcctgaggct
     1801 ggcggcgcgg gcgatgccgg accgttcatc gacctgctgc ttgagatccg cgccgcgctg
     1861 cgcgccgaga agaactggac gctgtccgac gcgatccgcg acggactggc agagcgagga
     1921 gtgacggttg aggactcgcc gaccggggca acctggcgcc ggagggacta agcgcaacag
     1981 cggcgggaga ccggagcggt cggggaaacc gggtcgctca ggctccggcc gaccggccac
     2041 cgaacacggt ggtggccgcc gccagcctga gcgtcgcgac cgcccgccac ggcgggacga
     2101 cggcaacgac ctgctttacg gacgcaacgc agtccgggaa gcgctacgag cgggccgccg
     2161 ccgcttcatc agactgcttg tcaatgaggg tgccgccgac agccagcggt tgaccgagat
     2221 cgtcacatta gcgcgccaac gggatgtcgg aatcgagacg gtctcgcgcg atacgctgga
     2281 cgcaaagacg cccggccagc atcagggcgt cgtccttgaa gcgagcacct acccgtacgc
     2341 cgtcggcgat gaactgcgcg aactcgccga agatcaggcg attctgctgg cgctggatgg
     2401 tatcgccgac ccccagaacc tcgggacgct gttacgaacg gctgaggcaa ccggcgttcg
     2461 gctgatcgtc atcccgtccg accgcgccgc ccaggtgacg ccggccgtcg tcaacgcctc
     2521 agccggcgcc gtcgagcacc tgcatgtcgc ccaggaggtc aacctcactc gctggctggg
     2581 ccgagcgaag gatgcgggct tctggatcgt cggaatggac ggcgaggacg ccgagccgct
     2641 gttcgacacc agcatgcgcc caccggtcgt cctcgtcgtc ggctccgagg gcagcggcat
     2701 ccggcgactc gttcgagaaa cctgcgacat catcgtcgcg ctgccgatgc tcggacgaat
     2761 cgagtcactc aatgcggcgg tcgcggggtc gatcgggctg tatgagatcg ttcgcgacgc
     2821 tgaggaagat tgaagccgga accataaaag cgatacactc agtcgctcag gcgtgtccga
     2881 accggtttcg ggctgaccga gcattgcggg ccaggttcca tcgtcatgcc cgccgacgga
     2941 tgcaacagat ctggaaggtt attcgcgtca tgaagatcat tctcacgcag gacgtaccca
     3001 agcttggatc gtccggcacg gtccaggatg tcaagccggg ctttgcgcgc aactacctga
     3061 ttcctcaggg catggccgtc atcgcgaccc ggggctcgat caagcaggtt gaggagcgcc
     3121 aggccgccga cgcgcgccgg atcgccaagc aggaagagga gctgcgcggt ctctccgagc
     3181 ggctgcaggg gatgcgcgtc cagatcgagg cccgcgttgg cgagcagggc cgcctgttcg
     3241 gctccgtcac ggctgccgac atcgccgaga aggtgtcggc gcaggtcggc gaggagatcg
     3301 accgccgcaa gatcgatctc gacgagccga tccgcaccgt tggcgagcac gcagtcacga
     3361 tccggctggt tggccggctc cagccgacga tcaacgtcgt ggtctgggac ccggagaacc
     3421 cgcacgcatc cgtcaccgat gagccggcag ccgaggctgc tgccgagggt gacgaagacg
     3481 tcgatggcga cgcggtcgcc gtcgtggcgg ctgaggacga gaacgacacc gaaaccgagt
     3541 aatgcccgat gatgcaagcg gtcgatcggc tgccacccca caatctggag gcggagcaat
     3601 ctgttctggg gtcgttgctg atcgaccggg atgcgatcat ccggatcgcg tcctatgtca
     3661 agtcgagtga cttctatcgc gccgcccacg ggctgatcta cgaagccatc gtcacgctct
     3721 acaacaagcg cgagccgacc gacctgatca cactggttga cgagttgcga cgtaccaacc
     3781 ggctggaaga tgccggcggc gagtcgtacc tgagcgaact gatcgcggcc gtcccgaccg
     3841 ccgtccatgt cgagtactat ggacggatcg tcgagcgaac ggcaacgctc cgtcgcctga
     3901 tcagcgccgg gaccgagatc gtcgacatcg gctacaccga gtcgatggac atcgaacagg
     3961 cactcgatgg cgccgagaag gcgatctttg ccgtctcgca gaagcgctcg acccgcgatt
     4021 tccgctcgat cggcgagatc ctcgaagagt acttcgaccg gctcgacacg ctccagcaga
     4081 acaagggcgc tgtcgtcggc gtcccgaccg ggttcatcga tctcgacaag ctgaccggcg
     4141 gcctgcagcg ctccgacctg atcatcatcg ccgcccgccc tgctgtcggg aagtcatcgc
     4201 tgggactcgg cttcgcgttc aacgcggccg tccgtttcca gaagacggtc gggctattcg
     4261 tgctggagat gtccgccgaa cagatcgtcc agcgcatgct ctcgatggag accggcatcg
     4321 actcccaccg gctgcggatg ggctacatcg acgacagcga gtggagcagc gtcacccgcg
     4381 ccttcggccg gctggctgag gcgaacatct tcatcgacga caccgccagc gcgaacatca
     4441 tggaagtgcg ctcgaaggcg cgtcgcctcc acgccgaacg cggtctcgac ctgctcatca
     4501 tcgactacct gcaactgatg accgcctcac gggcggacaa ccgcgtccag gagatctcgc
     4561 agatctcccg cgccctcaag ggcctggccc gcgagctgga tgtcccggtc gtcgccctcg
     4621 cccagctctc ccgcgcagta gagacacgtc agtcacatgt tcccatgctg tcagatttgc
     4681 gcgaatctgg ttcaatcgag caagatgcgg atatagtcgc atttatctat agggaagatg
     4741 tgtacgatcc agacaccgat cgcaagggca tcgccgacct gatcatcgcc aagcaccgca
     4801 atggcccgat tgggactgtc cacctgcgtt tcttcgatcg tacggcacgc tttgccgacc
     4861 tcgaactgta tcgcgagccg gacctgtagg ctgagaatcg caatctcctt acgaacggac
     4921 gggacgtact gatgcccgac ggcgatagcg ccgtacctga cgatttgata cctgccgcgg
     4981 tcctcagccg cctggttcgc gaggtctggt cgccgctgga ggtcaagctc gtgctggccg
     5041 tcgccgttct cggcgggttg catgatccgg tcagggaaga gtcgctgctg atcgacgagg
     5101 ggttggcggc agggatgcgc gccgatggct cttcgcgtgg gacggtcgag cggctattgg
     5161 aaacgatcga gttggcaacc gcccgtggcg tcctgctgcg actggtcgat gacgacggcg
     5221 actactggct gatcctcggc gtcgacgcca accggcgcaa ggtccggagc gggctggtgt
     5281 tcgccggcga gacggctcaa cgctggcagg gtacactccg ggttgaacga ccgagtatat
     5341 tcacactgta cgaacagaac gtcgggctgg tgacgccgat catcgccgac cggctggttg
     5401 aagcgatcga gcgctacccg gaagcctggg tcgaggacgc aatcgaagaa gccgttgcct
     5461 acaaccgacg caattggcgc tacatacaac gcattctgga gaactgggca tcggagggac
     5521 gcacagatga ggccaatcgg ggagatcctg cgcgggatct caatcgagac aaacacctcc
     5581 gcggcaaata cgcacacctc ttccgacgag acaacctgcc cgatctgtaa cggcgccgga
     5641 tatctgcgga tggacgcatc cgtcaccagc gccaacttca gccggctcgt cccctgcacc
     5701 tgcaaggtcg aggagcgacg ccagcggtcg cagaacaacc tgcgggtgtt ctcggacctg
     5761 tcgccgttcc gcgatcagac ctttgactcg tttgatcccg gcgtccgcgg tacccgcgac
     5821 gcgttcgagg ccgccgccgg ctatgctcgg caccccgatg gctggctggt gcttgttggc
     5881 gattacggtt gcggcaagac gcacctggcg gctgcgatcg ccaacttcgc actcacggag
     5941 cgggagatgg ggccgatcgt cgcggttgtt ccggatctgc tcgactacct gcggacgacc
     6001 ttcagcccgg actccgatgt ctcgtacgac gatcgtttca acgccttccg gggggcggat
     6061 ctgctgattc tggatgacct cgggaccgag aacgcgacac cgtgggcacg cgagaagctg
     6121 tttcagatca tcaatcaccg ctacaacgag cgctcgccgc tggtcgtgac gaccaatgac
     6181 ctcgaccgtg ttgacccacg gattcgttcg cgcctctgcg actggcaact ctcccgtcat
     6241 gtctacatcg acgcggatga ctaccgggac agaagtggta gccggcgacg gctccgtacc
     6301 aatggcaatg ggcaaaaacg gtcatgaagt actatgtcgg aggtgcaagt cgctccccta
     6361 tactcaacgt gcggttggac gttgtcgtcc agccgagcca tgagtgctgg aggcaggatg
     6421 agcggcgccc ggatgaccaa agacaacgac ctgagcctga cacaatctga cttccgacct
     6481 caaggcgaga tgtacctgat gccagatccg aaccagccac ctgccgaggt gatctccttc
     6541 aaccggaccc cggcgcaatc cagcgacccc aacgagtcat tcgaattgcg cgacatggtc
     6601 agcctcgcga tcacctattt cgatcgcggc atgatcgacg acgccgagga gatgttgaac
     6661 gaggcgctcg aagccggcta ttcgcgacag gacgcggttg atcttctgcg tcgggtccag
     6721 tcagttcgcg gccagacgac gatggttggc gccgcgcgta cagctcattc tgaagctgaa
     6781 caacaggcga ccgacagcgt cgtccacgag ttcacgatgc cgttgccggg cgtcgattcg
     6841 cagaccagcc tcgtgcgtcg cgctatcgag gatgccgatc gtgacctggc tgccgggcga
     6901 cttgagtcgg cccacgacgc gaccctgcat gcgcttgcga tggccccatc ctatttgccg
     6961 atctacgtac gcctcgccga gatccgcacc gcgtttggcg acagtgagag cgccgagacg
     7021 ttgatcgggt cactcaaggc ggtgctggag tcgatcggcg acgagcgcga ctggctgacg
     7081 cagtcgatgc gcgtcacgct cgatcccgac aacaccgacg cactcgtgca gctggcgcgc
     7141 tcgctggtcg aacagaaggg cacgctccag ctggagccgt atgtccccga agcgatcgaa
     7201 cgcacgctcg ctgacaaccc ggaagtggcg cttgagcttg cgcgcgagta catccggctc
     7261 cggccgaaca tgcgtcaggc ccagcggcta cacctccgcg cggtcgtcgc cgaaggcgat
     7321 atcgcgcagg taacgtcgct gctggagcgc gaagtcacgc cggacgctcc cgccgacctg
     7381 ctcttcctac gcagctgcgt ggcacacagt gagggccggg agacgtggtt ccactggctt
     7441 gagcgagcgg tctctgtagt tctgggcggc gactacgacc aggacgacct cgctcgtgcc
     7501 gtcgatgcgt cacgcaatct gctgcccaat ccgcagcacg cgctcgccac cagcgtcgtt
     7561 cggatggccg gcaacgatcc gcatggagcg ctcgccgtcc tcgatccgtg gtcgggtatc
     7621 ccaggccggg agacatccaa cgccagcgag atgctgattg cggcctgcgc tcgcgcgttt
     7681 tcactgcggg ccatctcgcc gatcgagtcg atcgaggcgc tttcgaacgc agtcggccag
     7741 gcggtcgtcc ttgatgttcg gccattcgca gaggcgtgca ggctgtttgc ccgctcgatt
     7801 tcggccgagg cgctgatgca tgaactggta gccgtcgtcc gcgagaccgg ccagcacgag
     7861 atggcgatca ggcagctcca ggcgctgcgc gaccggatgc cggagcacct cgagattcgg
     7921 accggactgg cggacctgca agtcgcggcc ggccggacgg ccgagggcgt tcgcgaattg
     7981 cgctacatcg ccgaacgcta cgagcaggcc ggcaacatcg atcgaatggt cgacgcgatg
     8041 cggcatatct cggccgccgt cccaaacaac gtcgagatga aggcgaagct gatcgagggc
     8101 tacgtccagc gcggtgtgcc ggaagacgcc atgcgcgaac tgcgcttgct cggagacctg
     8161 catcacaagc gcggccggat ggccgaagcc gccgccgcct acacccgtgg cgcggagatc
     8221 gcatcgacga ccggcaatta ccggcgggcg atggacctct tcgagcgggc aatcggcgcc
     8281 gatcccgaga acgtgggtgt gcgccacgcg gccgtcgcct actacatcat ggtcggctcg
     8341 gtcgacaagg caaccgagca gctgcgcgag gtcgtccgga tcgcgctcca ggagcaggac
     8401 ccggacgagg ccgtcgccgc cttgcatcaa atcatcggcc tgtcgcctgc cgaagcgtct
     8461 gcctaccaca agcttggtga ggtgctgacc tcgctcggtg agtacgcgca ggccgagcgt
     8521 gtctatcgcc ggctggcggc gttcacgccc gacgatccgg ttctgacggc caagcagtcc
     8581 gcactggcgg cgctggcagc cggtcagtag ccgcaaccgg cgcgcgagtc aggccactca
     8641 ggcgctcgtc gtatacaatc aaggtcgtgg ctcgactgcg ttactgcatg tcttaggtgg
     8701 accatgcccg aactgccctg gattttcacc aggatcaccg ccagggccct ggttgatatc
     8761 tccgcggtgg cgctgatcat ttactggctt ctctggtttg ctcagcgaac acgcgcggca
     8821 caactgatcc gcggactgat tatcctgatc gtcggcgtcc tgctcgcgtc gaacatcttc
     8881 gacctgacgg cgctgaactg gttgttctcg cgcgcctggc cggcgatgat cgtcgccatt
     8941 cccatcatct tccaacctga gcttcggcga gcgctggaac agctcggcca caccggctca
     9001 tggattccga agccattcgg ccagcccgtc gatgccgacg tcgaccggac gattgacgaa
     9061 atcagccgag cggctgctca tctgagccgg atcggctatg gtgcattgct cgtcatcgag
     9121 cgggagactg gcctgcagga ttacgccgac cggggtgtca cgctcgacgc cgtcgtcagc
     9181 cggcaacttc tgatcaacat cttcttcccg aactcgccgc tgcatgacgg cgccgtcatc
     9241 gtgcgcggca accggctact ggccgccaac tgcatcctgc cactgagcga ccgcgagatc
//
LOCUS       DASUGJ010000350         4210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1165812, whole genome shotgun sequence.
ACCESSION   DASUGJ010000350 DASUGJ010000000
VERSION     DASUGJ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165812"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      292..376
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            622..3936
                     /locus_tag="VFV93_07795"
     CDS             622..3936
                     /locus_tag="VFV93_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HEX5165281.1"
                     /translation="MTETEVADGAILIAHLVAVDFESVSTIGICPVDAGWTLAASVPG
                     NDAVVQFIWWRSVTAAQTAAVSYTFESRADGSCSTEDEVVTALTGAISVYSGVATDSP
                     IGVVASSSLSDQGVAAIAPEIAASQPAGSRVLRFLGIDNDRELALADSSLRYSVGLST
                     EGGPRRAALYDGLLSAEGPVAEQSVGFRSRSRWVATTVVLREPSESAPTDTTPPQTTA
                     SSPSYVAGTWTNATAVDVVLSASDAGGSGVSAILYSLAGSQTQDLTTVPGATANVSIT
                     AEGETTISYFAHDVAGNDEVMQTFVVRIDRSGPSLSGAPTTAPNGDGWYSGDVTIAWT
                     CSDGVSGIAGSCPAESVIGGEGENLSAGATVRDIAGNERVASVAGINIDRTAPVTTIE
                     PPSGWSNSPVTVTFSADDNLSGVQLTSYTVDDGPEQSGGAVTFDQSGRYSLRYWSVDN
                     AGNREEAKQVEILIDRDAPHISHTLSSEPNAYGWHTAPVTVTFTCEDTVSEIVSCSDP
                     QTLATDGESQTVTGTAVDAAGNVANDAVSLKIDQTPPVITASRTPAANASGWNATSVT
                     VSAECSDATSGIRECPAAETIDEGANQQVTLTAIDVAGNSTSATVSGINVDLTAPTIT
                     ATATTPDGATYLAGSWASQPVTVTFSCSDVLSDIASCPEPQVFGEGAAQSAAGTAEDI
                     ARNQASAKFGPINIDLTAPTIQAVLTTAGGDPYTPGDWANGPVTVTFVCDDALSLVAT
                     CSEPVTLGDGIGQRVTGTVTDVAGLTASVTIEQINIDSVAPTITATATTADGRQYVSG
                     LWTNQPVTVSFACVESLSGLAQDCPEPIVVSDSTPIAGQAVSAGISDRAGNAASSDVI
                     VVMLDRDAPRFTFVPDDQRVEVRGVSETVVNWPDPTAVDDLSGTSGPVCLPASGAIFG
                     LGETPVTCTATDGAGNVATAAFTVTVIDVQPPDLNIPDNMTVPADGPQGAVVHYSVGG
                     EGLRIAAAPDCTPASGSRFQVGTTVVECHASNPSGVSATGSFTVTVLGASDLLGGLRG
                     GTIDAVSDRTAERALLRTLDQVDVALRSGNSIRAVFGMLQFWIQVDLYARHGRIAAVD
                     LRQLHTGVRQVIDAIW"
     gene            4019..>4210
                     /locus_tag="VFV93_07800"
     CDS             4019..>4210
                     /locus_tag="VFV93_07800"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165282.1"
                     /translation="MTGLRFRRRGAVATAGVDMPRFRPQMFASLRHRNYRLYWIGSLV
                     ANTGDWMDQIAIGWLIWELT"
BASE COUNT          748 a         1331 c         1413 g          718 t
ORIGIN      
        1 gtctcgccaa actgggtgct gaaccacctc gtccagcacg aagccgagca ccgtgccgag
       61 atatctctcg tccttgccgt cttcgaaggc ggcagatatc agtgaggtgg ggtatcagga
      121 ctagctcggt ctgcgccggt aaaggttcgg gccgtgctta ccaactgtga caatctggct
      181 ctttacagat gcagccgtca cacatacaat gttcgtgcca atgatacaga tgatgtatgc
      241 gcgacatggt cgcggtcatg gtggggtgtc ggcgggctct atcggcgaac gggtccatga
      301 ggatgtgatg atgcgcgggc tgtaatctgg acgcctcgtc cgcgttgaac tggtggcgta
      361 accggcctga cggttgtcgg ccggttttgt gttgtccggc gctgaaatat tcggggaggg
      421 gtgatcgtgg cgcgtcgtgc catttccgct gtgctgtccg gcaggcatct gattggtgca
      481 gttgtcgccg ttttgatgct ggttggtctg tgggcaccat tcgtttcgcc ggcccgcgtc
      541 gctgcagata tcgcgcctcg tttgttcacc caaagttcca ataccggtga gtccagttcg
      601 gtgacgattg cttctccgaa tgtgacagag actgaggtcg ctgatggcgc catcctgatt
      661 gcgcatctgg ttgcagtcga ttttgagagt gtcagcacga tcggcatttg ccctgtcgat
      721 gccggctgga cgctggccgc gagtgtgccc ggcaatgacg cggttgtgca gttcatctgg
      781 tggcggtcag tgacggcggc acagacagca gcggtcagct acacctttga aagtcgcgcg
      841 gacggcagtt gcagtaccga ggatgaggtc gtgacggcgc tgaccggcgc gatctcggtc
      901 tatagcggcg tcgcgaccga cagcccaatc ggcgtggttg catcaagctc gctttcggat
      961 cagggcgttg cggcaatcgc gcctgagatt gcagcgtccc agccagcggg atcgcgtgta
     1021 ttgcgatttc tgggcatcga caacgatcgg gaactggcgc ttgcggacag ttcgctgcgc
     1081 tactcggttg gcctgtctac ggagggaggg ccgcgacgtg cggccctgta tgacgggctg
     1141 ctgagtgccg aagggccggt ggcggagcag tcggtcgggt tcagaagccg gtcacgttgg
     1201 gttgcgacga ctgttgtatt gcgtgaaccg tctgaaagtg caccaaccga cacgacgccg
     1261 ccgcaaacga ccgccagcag cccatcgtat gtggccggta cctggacgaa cgcgaccgcc
     1321 gtcgacgttg tgctgagcgc cagcgacgcg ggaggctccg gcgtcagcgc gatcctctac
     1381 agcttggccg gcagccagac ccaggatctg accacagttc ccggtgcaac agcgaacgtc
     1441 tcgatcacgg ccgagggcga gacgacgatc agctacttcg cgcacgatgt cgcgggcaac
     1501 gacgaggtga tgcagacctt cgtcgtgcga atcgaccgaa gtgggccgag cctgtccggc
     1561 gcgccgacga cggcgccaaa tggtgacggc tggtacagcg gcgacgtgac gatcgcctgg
     1621 acgtgcagtg acggcgtgtc ggggatcgca ggcagctgcc cggccgagag cgtcatcggt
     1681 ggcgaggggg agaacctctc cgccggcgca acggtccggg acatcgccgg caacgaacgg
     1741 gtcgccagcg tggccggcat caacatcgac cgaacggcgc cggtgacgac gatcgagcca
     1801 ccctccggct ggagcaacag ccccgtgacg gtgacgttca gcgccgacga caatctctcc
     1861 ggcgttcagc tgacgtccta caccgtcgat gacgggccgg agcagtcggg cggcgcggtg
     1921 acgttcgacc agagcgggcg atacagcctg cgctactgga gcgtggacaa cgcgggaaac
     1981 cgtgaggaag ccaagcaggt cgagatcctg atcgaccgtg acgccccgca catcagccac
     2041 acgttgtcat ccgaaccgaa cgcctacggc tggcacacgg cgccggtgac ggtcaccttc
     2101 acctgcgagg acaccgtctc cgagatcgtc agttgcagcg acccgcagac actggcgact
     2161 gacggcgaat cccagaccgt gaccgggacg gcagtcgacg cggctggaaa cgtcgccaat
     2221 gatgccgtca gcctcaagat cgaccagacc ccgccggtca tcaccgccag ccggacaccg
     2281 gcggcaaacg ccagcggctg gaacgcgacc agcgtcaccg tcagcgcaga gtgcagtgat
     2341 gccacctccg gcatccgtga gtgtccagcc gccgagacga ttgatgaggg cgcgaaccag
     2401 caggtgacac tgaccgccat cgacgtggcc ggcaacagca ccagcgcgac ggtgagcggc
     2461 atcaacgtcg acttgaccgc gccaacgatc accgcgaccg ctacgacgcc cgacggcgcg
     2521 acgtatctgg ccggcagttg ggccagccag ccggtgacgg tcaccttctc gtgcagcgat
     2581 gtcctgtcgg acatcgccag ttgccccgaa ccgcaggtct tcggcgaggg cgccgcccag
     2641 agcgcggcag gcacggctga ggacatcgcc aggaaccagg ccagcgcgaa gttcggcccg
     2701 atcaacatcg atctcaccgc gccgaccatc caggccgtcc tgacgacggc cggtggcgat
     2761 ccgtacacgc ccggcgactg ggcgaatggt ccggtgacgg tcacgttcgt ctgcgacgac
     2821 gcgctctcgc tggtcgcaac ttgcagcgaa ccggtgacgc ttggcgacgg aatcggccag
     2881 cgcgtaaccg gcactgtcac ggatgtcgct ggcctgacgg cgagcgtcac aatcgagcag
     2941 atcaacatcg acagcgtggc tccaacgatc accgcgacgg cgacgacggc cgatggacgg
     3001 cagtacgtct cagggctgtg gacgaaccag ccagtaacgg tgtcgttcgc ttgcgtcgag
     3061 agcctttcag gactagcgca agattgcccc gagccgattg tcgtcagtga cagcacgcca
     3121 atcgcggggc aggccgtctc ggccggcatc agcgaccgag ccggaaacgc ggccagcagc
     3181 gacgtgatcg ttgtcatgct cgatcgggac gcgccgcgct tcacgtttgt gccggatgac
     3241 cagcgtgtcg aggttcgggg cgtgtcggag acggtcgtga actggcccga tccaactgcc
     3301 gtcgatgatc tctcagggac gtctggtccg gtctgcctgc cagcgtccgg cgcgatcttc
     3361 ggccttggcg agacgcccgt cacctgcacg gccaccgacg gggccgggaa cgtcgcgacg
     3421 gccgcgttca ccgttacggt tatcgacgtc cagccacccg acctcaacat acccgacaat
     3481 atgaccgtcc cggccgacgg tccgcagggt gcggtcgtcc actacagcgt aggcggcgag
     3541 gggctacgga tcgctgccgc gccggactgc acgccggcct ccggcagccg cttccaggtt
     3601 ggaacgaccg tcgtcgaatg ccacgccagt aacccatccg gcgtcagcgc gaccggcagc
     3661 ttcactgtca ccgtcctcgg cgcgtcggac ctgctgggtg ggctgcgcgg cggaaccatc
     3721 gatgccgtca gcgaccggac cgccgaacgc gcgctgctgc gaaccctcga ccaggtcgat
     3781 gtggcgctgc ggtcgggcaa cagcatccgt gccgtcttcg ggatgttgca gttctggatt
     3841 caggtagatc tctacgccag gcatggccgc atcgccgccg tcgatcttcg ccagctacac
     3901 actggcgtcc ggcaggtgat cgatgcgatc tggtagacag tagccgattg acgccggtca
     3961 ccgcgaatgc gagactgcgc caagctgaga gggtggcgtg gctgaaggac ggttgcgggt
     4021 gacgggtctg cggttccggc gacgcggcgc ggtggcgacc gccggcgtcg acatgccccg
     4081 cttccggccg cagatgttcg cctcgctgcg ccaccgcaac taccgcctct actggatcgg
     4141 ctcgctggtc gccaacaccg gcgactggat ggaccagatc gcgatcggct ggctgatctg
     4201 ggagctgacc
//
LOCUS       DASUGJ010000351         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1859671, whole genome shotgun sequence.
ACCESSION   DASUGJ010000351 DASUGJ010000000
VERSION     DASUGJ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859671"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFV93_07805"
     CDS             complement(<1..297)
                     /locus_tag="VFV93_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEX5165283.1"
                     /translation="MEDIQIQVGNLRFTARFEREKSPKTCAAFEKLLPFRSKIIQARW
                     SGEAAWVPLGDFDLGVGEETATTSPAPGEILFYPAGISETEILFPYGQASFASKF"
     gene            433..1896
                     /locus_tag="VFV93_07810"
     CDS             433..1896
                     /locus_tag="VFV93_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5165284.1"
                     /translation="MAELDQSRAPFFDALAAYAGLEAVPFSTPGHKRGRGAPDDLRTL
                     LPDALALDIPHGGGVDTTHFSLGLLREAERLAAEAYGGDAARFLVNGSTTGNLAILLS
                     TCGNGDTVIVSRMLHKSLLTGLIFSGARPHYLAPAIDDDRNLPLDIEPAAVAGALDVY
                     PDAKAVVIVSPSYVGVSSDLAAIAAICHGRGVPLLVDEAWGPHFRFHPDLPPSAMECG
                     ADAAVSSTHKMLAGLTQSSTLVARAGRLELDRMSTIVDMVQTTSPSALIFASMDASRR
                     QMALDGRRLLDGTLAVAQRMRTGLAAIDGLEVLSPALVADRPGAGFDPTRVIVDVHGL
                     GLTGYEAEHVLRNEHGVYVEMSDLLSVMLLVTIGDDERSIDRAVAGFQSLTTLRKPPR
                     HAAAARSTGDLLFARLAELTPQQAFTAPAEVVPVGQAAGRISTESITPYPPGIPLVAP
                     GERLTQDVIDYLRAGIEEGMYVSGLADASFATVRVVR"
     gene            complement(1893..2303)
                     /locus_tag="VFV93_07815"
     CDS             complement(1893..2303)
                     /locus_tag="VFV93_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5165285.1"
                     /translation="MAATAATLRMDGKRSMMPLTWIGNSSQTMVYGSLVLDITTEQAS
                     RDGDEVALQQSDWAILNQLAAANNTFVRGSDLLESIWGDDMRDDALFLRSWVQRLNDR
                     LCRGSGCQIIDTLPGSYRLISPDECGTELDEFAG"
BASE COUNT          432 a          816 c          820 g          457 t
ORIGIN      
        1 gaacttgctg gcgaatgatg cctgcccgta tgggaacaga atctccgtct cggagatccc
       61 ggccgggtag aagaggatct ctcccggtgc gggcgacgtc gtcgcggtct cctcgccgac
      121 gccgagatcg aagtcgccca gcggcaccca ggccgcctcg ccgctccagc gtgcctggat
      181 gatcttactg cggaacggca gcagcttctc gaacgccgcg caggtcttgg gcgacttctc
      241 ccgctcgaac cgcgccgtga agcgcaggtt accgacctgg atctgaatgt cctccatcgc
      301 ccgtactccc ccctcgttct ccgtgctggc atatcgcgag aagcctacac cgtccctaac
      361 gcgctgaacg tgaagcgtag tatcgtctgc gagttggatg accgctgcga catcggttag
      421 gcgacaaaag acatggctga attggatcag tcgcgcgcac cgttcttcga cgcgctggca
      481 gcctatgccg gcttggaggc ggtgccgttc tcgacgccag ggcacaagcg cgggcgcggc
      541 gcgccggacg accttcggac gctgctgccg gatgcgctcg cgctggacat tccgcacggc
      601 ggtggtgttg acacgaccca cttctcactc gggttgctgc gcgaggcgga gcggctggcg
      661 gcggaggcct acggaggcga tgccgcgcgc ttcctcgtca acggcagtac gaccggcaat
      721 ctggcgattc tgctgtccac ctgcgggaac ggcgataccg tcatcgtctc gcggatgttg
      781 cacaagtcgc tgctgaccgg tctgatcttc tctggcgcgc ggccgcacta cctggccccg
      841 gccatcgacg acgaccgcaa cctgccgctc gacatcgagc cggcggcggt cgccggggcg
      901 ctcgatgtct accccgacgc gaaggccgtc gtcatcgtca gtccatccta cgtcggcgtc
      961 agcagcgatc tggccgcgat cgccgccatc tgtcacgggc ggggcgtgcc gttgctggtg
     1021 gatgaggcct gggggccgca tttccgtttt catcctgacc tgccaccgtc agcgatggaa
     1081 tgtggcgccg atgcggccgt ctccagcacc cacaagatgc tggccgggct cacccagtcg
     1141 tcgacgctgg tcgcgcgcgc tggccggctg gaactggaca ggatgagcac gattgtcgac
     1201 atggtccaga cgaccagccc gtcggcattg atcttcgcat cgatggatgc cagccgccgc
     1261 cagatggcgc tggacggtcg acgactgctg gacggcacac ttgcagtcgc ccagcgcatg
     1321 cgaacgggac tggcggccat cgatggactc gaggtgctgt cacccgcgct cgtcgccgat
     1381 cgacctggag ccggattcga tccgacgcgg gttatcgttg atgtccacgg cctggggttg
     1441 accggttacg aagccgagca tgtgctacgc aacgagcacg gcgtctatgt cgagatgagt
     1501 gacctgctct cggtcatgtt gctggtaacg atcggcgacg acgagaggtc gatcgatcga
     1561 gccgtcgcgg gattccagtc actgacgacg ctgcgcaagc cgccacgcca cgctgcagcg
     1621 gcccgatcaa caggcgatct gctcttcgcg aggctggcgg agttgacccc gcagcaggcg
     1681 ttcacggcgc ctgccgaggt cgtcccggtc gggcaggcgg ccggccggat cagcaccgag
     1741 agcatcacgc cctacccgcc cggcatcccc ctcgtcgcgc ccggcgagcg gctgacgcag
     1801 gatgtcatcg actacctgcg ggctgggatt gaagagggga tgtacgtctc cggcctggcc
     1861 gatgcgtcgt tcgcgactgt ccgggtcgtg cgttagcccg cgaactcatc caattccgtg
     1921 ccgcactcgt ctggcgagat cagccgataa ctgccgggaa gcgtgtcgat gatctgacag
     1981 ccggagccgc ggcagagccg gtcgttcagc cgctggaccc aggaacggag aaacagcgcg
     2041 tcgtcgcgca tgtcgtcgcc ccagattgac tccagcagat ccgacccgcg tacgaaggtg
     2101 ttgtttgccg ccgcgagctg gttgaggatt gcccaatcgg attgctgtaa cgcgacttca
     2161 tccccatcgc gggatgcctg ctcggtcgtg atatcgagga cgagcgaacc gtagaccatc
     2221 gtttgcgagg agtttccgat ccaagtcagc ggcatcatcg aacgcttccc atccatccgc
     2281 agcgtcgcgg ctgtcgcggc caatctccat cctcatccta gtgagaaatt tcacagatgg
     2341 gaagtgggtt gcggtggtct gaaacacaat tgtgacgggc gctccatgct acgatgcctc
     2401 cggtcgaagt gtggatagcg gaagtgggag aaacagcatg cgcgcgaatc gactacggga
     2461 gttgctcacc gaaggtaacc cgtcgctcgg aacgcggcta cacaacatct ggccgggagt
     2521 gaccg
//
LOCUS       DASUGJ010000352         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1304363, whole genome shotgun sequence.
ACCESSION   DASUGJ010000352 DASUGJ010000000
VERSION     DASUGJ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304363"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFV93_07820"
     CDS             complement(<1..559)
                     /locus_tag="VFV93_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5165286.1"
                     /translation="MPRVIGIDLGTTNSAMAVLEAGEPTILENAEGNRVTPSVVAVNP
                     KTGERLVGTIARRQAVTNPENTIFSIKRFMGRKFDDPVVQEAIERVPYKVTRAPNGDV
                     QVVMGGKEYSPPEISAMILQKIKADAEARLGDKVNQAVITVPAYFNDAQRQATKDAGQ
                     IAGLEVLRIVNEPTAAALAYGLDKVG"
     gene            complement(597..1202)
                     /locus_tag="VFV93_07825"
     CDS             complement(597..1202)
                     /locus_tag="VFV93_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5165287.1"
                     /translation="MAEPEDQMLASEPAESPAERQASADELLEWLRRERADFLNYKHR
                     VERERAVDARRTQADTVRQLLNVLDDLGRALAQPPPELKLHPWVQGVALTNRTLIETL
                     RALGVERIGQPNEPFDPERHEALVYNPLPGATEQRVESVIRPGYQLGETLLRPAQVVV
                     VGPGGARGEAPGTIPASDSIDEMTDEMRELHDADDARGPQA"
     gene            complement(1202..2059)
                     /locus_tag="VFV93_07830"
     CDS             complement(1202..2059)
                     /locus_tag="VFV93_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165288.1"
                     /translation="MDLFGLLWLFFIISSLQPLIRQRWLEAQRVRAFKRIEKQRSSRV
                     IGLIHREERMSILGIPVARYIDIQDSEDLLQAIQMTDRSVPIDLILHTPGGLLLAAEQ
                     ISMALRGHAAKVTVLVPHYAMSGGTLIALAADEIVMGMHAVLGPVDPQLGQFPAASLV
                     KVIERKPIAEIDDETIIFSDVAQKALRQVECFVRELLSERMPAERAAVAATTLATGTR
                     THDYPITVQEARELGLPVSTSMPAEIFELMRLYPQTAQRRPSVEYLPTPYGPRDRLPS
                     RSGSSERST"
     gene            complement(2612..>3055)
                     /locus_tag="VFV93_07835"
     CDS             complement(2612..>3055)
                     /locus_tag="VFV93_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5165289.1"
                     /translation="RAYARMVDQVRPLPGARDLLNTLTSYGVPWAIATSGRAEVARPV
                     LATLGVLADVPIVTRDQVPHAKPDPDLFLAAADRLGVAAADTVVVGDSVWDLLAARRA
                     RALGVGLLSGGYGRAELEVAGAYQVYEDPADLLRRLDEVGIRGAV"
BASE COUNT          529 a          987 c          936 g          603 t
ORIGIN      
        1 ggccgacctt gtccagcccg tatgccagtg cggcagccgt cggctcgtta acgatgcgca
       61 gcacttcgag tccggcgatc tggccggcgt ctttcgtcgc ctggcgttgc gcatcgttga
      121 aataggccgg aacggtgatg accgcctggt tgaccttgtc gcccagacgt gcctctgcgt
      181 cggctttgat cttctgcaga atcatggccg agatttcagg cggggaatac tccttgccac
      241 ccatgacgac ctgaacatcg ccgtttggcg ctctcgtgac cttgtagggg acgcgctcta
      301 tcgcttcctg gacgactgga tcatcgaact tgcgccccat gaagcgtttg atcgagaaga
      361 tcgtgttctc cgggttggtt actgcctggc gtcgagcgat cgttccgacc agccgttcgc
      421 cggtcttcgg gttgaccgcc accaccgatg gtgtgactcg gttcccctcg gcgttctcga
      481 gtatcgtcgg ctcgccggcc tcgaggaccg ccatcgccga gttggtcgtt cccaggtcga
      541 taccgatgac tcgcggcatg ggtcactctc ctgttcccgt gaatacgacg acgctgctat
      601 gcctggggcc cgcgcgcgtc atctgcgtcg tgcagttcgc gcatctcgtc ggtcatctca
      661 tcgattgagt cagacgctgg gatggttcct ggcgcttctc ctcgcgctcc gcccggtccg
      721 acgacgacga cttgtgccgg ccggagcagt gtctcgccca gttgatagcc cggacggatc
      781 actgattcaa cgcgctgttc ggtagcgccg ggtagcgggt tgtacaccaa cgcctcgtga
      841 cgttctggat cgaatggctc gttcggctgg ccgatccgct ccaccccgag tgcccgcagc
      901 gtctcgatga gcgtccggtt tgtcagtgcg accccctgga cccagggatg caacttgagt
      961 tcaggcgggg gctgcgcgag ggcacgcccc aaatcgtcca gcacattaag tagttgccgc
     1021 accgtgtccg cctgtgtccg cctggcatcg accgcacgtt cgcgctcgac acggtgcttg
     1081 tagttcagga aatccgcgcg ctcgcgccgc aaccattcga gcagttcatc cgctgaagct
     1141 tggcgctcgg ctggactttc tgccggctcg gacgccaaca tctgatcctc cggctcagcc
     1201 attacgtgct cctctcgctc gacccggacc gagatggcag ccgatctcgc ggcccatacg
     1261 gagtcggcaa gtactccaca gacggccggc gctgcgcggt ctgcggatac aagcgcatca
     1321 actcgaatat ctcggctggc atcgaggtcg agaccgggag acccaactcc cgtgcctcct
     1381 gaaccgtaat cggatagtcg tgggtcctgg tgccggtcgc cagtgtcgtt gccgccacag
     1441 ccgcgcgctc ggccggcatt cgctctgaga gcaactcccg cacgaagcac tcgacctgac
     1501 gcaatgcttt ctgggcaaca tcactgaaaa tgattgtttc gtcgtcaatt tcggcgatcg
     1561 gcttgcgctc aatgaccttc acgagcgagg ctgccgggaa ctggccaagt tggggatcga
     1621 ctggaccaag taccgcatgc atgcccatta caatctcatc ggcggcgagc gcgatcagcg
     1681 ttccgccgct catcgcatag tggggcacga ggaccgttac cttcgccgcg tgcccacgta
     1741 gcgccatcga gatctgttcg gcggccagaa gcaggccacc aggggtgtgc aggatcaggt
     1801 caatggggac cgatcgatcc gtcatctgaa tcgcttgcag caaatcttct gaatcttgaa
     1861 tgtcgatgta tcgggcgacc ggaataccta gaatactcat tcgctcctcg cggtggatca
     1921 ggccgatcac ccggctggat cgctgcttct cgatccgctt gaatgctctg acgcgctgtg
     1981 cctcgagcca gcgctgccgg ataagcggct ggagtgagct gatgatgaaa aagagccaga
     2041 gtaggccgaa aaggtccatc gaccgtccca tcgcgtgtcg gcgctcgcgc gagtccggta
     2101 acgctcgacg caccgggcgt tttgtaactc tatgtggcgc aatccatggc tacatcggct
     2161 agaaggacca ttgcggttga ggatcgagaa taggcgatct actggtccat ctgggccaac
     2221 agggggcact ggcgtgcggc gcccagtaga taccgcgcag acgtagccag cggtaaccgt
     2281 ggccatcgat gcggatcgat gggttcgcta cgttgagtga tgcatcgtgg cgatcggaaa
     2341 gcagatcggt cgcggtctgg acgttctacg catccagttc caacggaccg tcggccgcat
     2401 ctcgggcctc cgccttgagc ggcccggtcc actggtgaac ctggtcgatg tggcccatgc
     2461 ggctggtgct ggagatgagc agcgaccttc agactgcgat gagtcgcgcc gacgtgctca
     2521 tggcgaagag gatggccagc tctggcgtgg caggacgcaa cgcgtttctg acctgcgtcg
     2581 ctccaattcg tcaggcaacc atgaactgca cctacactgc gccccggata ccaacctcat
     2641 ccaaccgccg caggaggtcg gccgggtcct cgtagacctg gtaggcacca gcaacttcca
     2701 actccgcgcg gccatagcca cctgagagca gcccgacgcc gagtgcccgc gcgcgccgtg
     2761 ccgccaggag atcccagacg ctgtcaccca ccacgaccgt gtccgccgcc gccacgccca
     2821 accgatccgc cgctgccagg aacagatctg gatcaggctt ggcgtgcggc acctgatcgc
     2881 gggtcacgat cggcacgtcg gccagcacgc cgagcgttgc cagcaccggt cgtgcgactt
     2941 ccgcgcggcc gctggttgca atggcccacg gcactccata cgaggtcaac gtgttgagca
     3001 ggtcgcgcgc acccggtagc ggtcggacct ggtcgaccat ccgagcgtag gcgcg
//
LOCUS       DASUGJ010000353         4970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_193925, whole genome shotgun sequence.
ACCESSION   DASUGJ010000353 DASUGJ010000000
VERSION     DASUGJ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193925"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VFV93_07840"
     CDS             <1..1040
                     /locus_tag="VFV93_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165290.1"
                     /translation="DYVAELRDSIADIREAMEGVRQAAEDYDDAVDDDIEEAANALED
                     ATDRIPTGATSEEASAEIADELAALDEAQAQASAELNCETTASASPTATGTAALTATV
                     EPSPTLTATVEPTATTEPPTQTPEPTATPEPTATLEPSPTPTEAPSPTPPPTVPPTPE
                     GTQWTETYVADWSAGPGNWVLPQGWLIENNALILEASVPGNALAPHQPAAPGYAVEVQ
                     MSATFGQEPPTCDNGGGILFSPTPEVKVTGTFPTGLLFSRCHQGWELSVVNAEGQRAT
                     LIAGQMPSDDAPHTFRIEVSQNAVRILIDGTTISGIVDERIGQVPFPGIYSTANYRIT
                     IASFRVFELAS"
     gene            1085..1807
                     /locus_tag="VFV93_07845"
     CDS             1085..1807
                     /locus_tag="VFV93_07845"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX5165291.1"
                     /translation="MIPSSPGTTRIDVERDSPGTLRFAAWPNSEHVEESAMHVRLGAT
                     VLDNNQVDVGRVERLVIDPGQRQIIQLVAKHGRVDPKHHIIDRGVVSRVDEEGRVHLN
                     VSEREVSGLREYYAVNYVKQSYQGEFSWSQAVGTPLGGPSQTISPGATEYTTLPENVV
                     VVTKGMDVQDSDFEKFGELEDIEFGQNAIVTGFTVRSGGMLRHDYHYLPVEHVAGVGE
                     DYVRVNLTGDEARAMPARSASD"
     gene            1870..3321
                     /locus_tag="VFV93_07850"
     CDS             1870..3321
                     /locus_tag="VFV93_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165292.1"
                     /translation="MGHDPPLDQATLGATDPGADTPEASSIGYRTVLRHREVRVLAVS
                     RAANKMAMSTVSYGAMVHLGQVGATQFQISLVSASSYLAALLFGVQGGTVADSLSKRL
                     ALAAGQGVMAGLCFAIPSLLGTSAGDLMLLMFLSSFLMQIVSPGLKAAVALVATPSEL
                     AVTSALVSVIGSVASGFGSAFLAPLLIKLSGIETVLYVSGMLYAVGSIRSLALAREQT
                     TPVAQALRGVDWRPTALSLRSNAQWIIDHRAVATMILGGAIVVALFEAFNTLIPLYVR
                     DVLDSDPANSVYIFAPAGVGFLIGTFLAPPLIQRWGERRLAIVSLIFMSASMAMFGLI
                     DAVAPILAPISPLRLLEPFGITLNDKVLAASVIAIPMNFGSTASGAAVANFINRRVPL
                     VRQGAVFGLEESQENALILATVLAVGAVAGVAGPQVVMVVAPIVAVSIVVLLLSYSYR
                     SVAQEHIAVRDAFDMLAADDNDRDTVPDHEVAR"
     gene            3335..4213
                     /locus_tag="VFV93_07855"
     CDS             3335..4213
                     /locus_tag="VFV93_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5165293.1"
                     /translation="MRQVTHALHRYRQSWAVTLVRNYLDDDADGLASIIAFAAMFSMM
                     PILVVTFLLLTLVLQINVIRDSLSGVLADEIPPAIAAPVEAIVQSGSGNLASLGIVTV
                     VAFFLGGTKLYSAIDRACARIFQTDRQRYAKRRLFALLMMPMIPTLLLIATSLSVIAT
                     AALALPIEEVVEVQPSTLESVLGYLLSYLVSFGLMLMAYWRIPERGPGLRCSVEGAAV
                     GALLIVVLAQSFPLYVQLTGGYNLYGSMFAFVLLLLLWLYLVGQIFVIGAEVAAFRSG
                     CRDDVSLSASQRSGPR"
     gene            4368..4898
                     /locus_tag="VFV93_07860"
     CDS             4368..4898
                     /locus_tag="VFV93_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165294.1"
                     /translation="MATKSVTERQAVDGQPVTENELRHKPVSKAIYGVITVLAVLQVM
                     EAHPPTGWSGAVFLFGTTVAVALVDAYSESIAIMLAQERRLSLADLRAIRHEVAPVLI
                     GAQAPTLILLASAFGLVSVELAITLAQVVAFVLLFSYGWRVGSLLHEHRLRQILSGLV
                     LVAIGGIAVGIKAAFH"
BASE COUNT          881 a         1570 c         1552 g          967 t
ORIGIN      
        1 ccgactacgt cgccgagctc cgcgatagca tcgctgacat ccgcgaggcg atggagggcg
       61 tacgccaggc ggccgaagac tacgatgacg ccgttgacga cgacatcgaa gaagcggcaa
      121 acgcgctcga agatgcaacc gaccgcattc caaccggagc gaccagcgaa gaagcgtcag
      181 ctgaaatcgc agacgagttg gctgcactcg acgaagccca ggcacaggcg agtgcagagc
      241 tgaactgcga gacgaccgcg tcggccagtc caaccgccac tggaaccgcc gcgctgacgg
      301 cgactgtcga gccatcgcca acgctgacgg cgacggttga accgaccgcg acaaccgagc
      361 cgccgacgca gacacccgag ccgaccgcga cgcctgagcc gaccgcaaca ttggaaccgt
      421 cgcccacgcc aacggaggct ccgtcgccaa caccacctcc gaccgtaccg ccaacgcccg
      481 agggtacgca gtggaccgag acgtacgtgg cggattggtc ggccgggccg ggcaactggg
      541 tcttgccgca aggctggctg atcgagaaca acgcgctgat tctggaagcc agtgtgcccg
      601 gcaatgcgtt ggcgccacat caaccagcgg cgccagggta cgcggtcgag gttcagatgt
      661 cggcgacctt cgggcaggaa ccgccaacct gtgacaacgg cggagggatc ctgttcagcc
      721 cgacacccga ggtgaaggtc accggaacgt tcccaaccgg actcctgttc agccgctgcc
      781 accagggctg ggagctgtcc gttgtcaacg ccgaggggca gcgagcgacg ctgatcgccg
      841 gtcagatgcc gagcgacgat gccccccaca ccttccggat cgaggtcagc cagaatgcgg
      901 ttcgtatcct catcgatggc acgacgatca gcggcattgt cgacgagcgg attggacagg
      961 ttccgttccc tggcatctac tcgactgcca actaccgcat cacgatcgca tcattccgcg
     1021 tcttcgagct ggcgtcctag atcggatcgg tgactgaact acgcccgtcc tttgcgactc
     1081 tcggttgatt ccgtcgtcgc ctggaaccac aagaatcgac gtagaacgtg actcgccagg
     1141 cacactgaga ttcgcggcct ggccgaattc agaacatgtg gaggagagcg ccatgcacgt
     1201 cagactcggt gcaacagtgc tcgataacaa ccaggttgac gtcggccggg ttgagcgact
     1261 ggtcatcgat ccaggtcagc ggcagatcat tcagcttgtt gcgaagcacg gccgtgttga
     1321 cccgaagcat cacatcatcg accgcggcgt ggtcagtcga gtcgatgaag aaggcagggt
     1381 tcacctcaac gtgagcgagc gcgaagttag cgggctgcgc gaatactatg cggtgaacta
     1441 cgtcaagcag tcctatcagg gtgagttcag ctggagtcag gccgtaggca cgccgctggg
     1501 cggcccgagc cagacgattt cgcccggcgc aaccgagtac accacgctgc ccgaaaacgt
     1561 cgtcgtcgtg accaagggga tggatgtcca ggattccgac ttcgagaagt tcggggaact
     1621 ggaggacatc gagttcgggc agaatgcgat cgtcaccggc ttcacggtgc ggagcggagg
     1681 catgctccga cacgactatc actacctgcc cgtcgagcac gttgccggcg ttggcgagga
     1741 ctatgtccgc gtcaacctga ctggcgatga ggcccgagcg atgcctgcgc gctcggccag
     1801 cgactgatcg accggcgatc tgctccatcg agtctgtgta tcgtcccagc gccgtaaagg
     1861 accggaccga tggggcacga tccaccgctt gatcaggcaa cgctcggggc gacggaccca
     1921 ggcgccgata caccggaagc gtcgtcgatc ggctatcgaa ccgttcttag acaccgcgaa
     1981 gttcgcgtcc tggcagtatc gcgggctgcg aacaagatgg cgatgtccac cgtctcctac
     2041 ggcgcgatgg tccatctggg tcaagtcgga gcaacacagt tccagatctc gctggtcagc
     2101 gcctcgtcat acctggccgc cctcctgttc ggagtccagg gcgggaccgt cgctgactcg
     2161 ctctccaaac gcctggcgtt agcggccggt cagggtgtca tggccggcct gtgcttcgcc
     2221 atcccctcgc tgctcggcac gagtgccggc gatctgatgt tgctcatgtt tctctccagc
     2281 ttcctcatgc agatcgtttc gccggggctg aaggcagccg tcgcccttgt tgcgacgcca
     2341 agcgaactgg ctgtcaccag tgcgctggtg tcggtcattg gttcggttgc gtcgggcttc
     2401 ggctcggcgt ttctggcgcc gctcctgatc aagctgtcgg gcatcgaaac cgtcctctat
     2461 gtcagcggaa tgctctatgc ggtcgggtcg attcgatcgc tcgcactcgc acgcgagcag
     2521 acgactccgg tcgcccaggc actgcgcgga gtcgactggc ggccgacggc gctttcgctc
     2581 cggtcgaacg cacagtggat catcgaccac cgtgccgtcg cgacgatgat tctcggcggt
     2641 gcgatcgtcg tcgccctctt cgaggcgttc aacacgttga ttccactgta tgtcagggat
     2701 gtgcttgatt ccgacccggc caacagcgtg tatatcttcg cgcccgccgg agtcggattt
     2761 ctcattggta ccttcctcgc gccgccgctg attcagcgct ggggcgaacg ccggctggcc
     2821 atcgtctcgt tgatcttcat gtcggcgagc atggcgatgt tcggcctgat cgacgccgtt
     2881 gcaccgatcc tggcgccgat cagcccgctg cgtctcctcg aaccgtttgg aatcacgctg
     2941 aacgacaagg tgctggcggc gtccgtgatt gcgatcccga tgaacttcgg atcgaccgct
     3001 tccggtgcgg ctgtcgcgaa cttcatcaac cggcgcgtcc cgctcgtgcg ccagggagcc
     3061 gtctttggcc tggaagaatc acaggagaat gcgctgatcc tggcaacggt tcttgccgtt
     3121 ggcgccgttg ccggcgtcgc cggtccgcag gtcgtcatgg tcgtcgcgcc gatcgtcgct
     3181 gtttctatcg tcgtcctgct gctgagctac agctacagat cggttgcaca ggagcatatc
     3241 gccgttcgcg acgcatttga catgcttgcc gctgacgaca acgaccggga taccgtgcca
     3301 gatcacgaag tggcgcggta gccatatgga cgagatgcgt caggtaaccc acgcactgca
     3361 tcggtatcgg caatcctggg ccgtaacgct cgtgcgcaac tatctggacg acgatgctga
     3421 cggactggcg tcgatcatcg cattcgccgc gatgttctcg atgatgccga ttctggtcgt
     3481 caccttcctg ctcctgaccc tcgtcctcca gatcaacgtc atcagggatt cgctgtccgg
     3541 cgtcctggca gacgagatcc cgccggccat tgcggctcct gtcgaagcga tcgtccagtc
     3601 tggcagcggc aacctggcca gcctcggcat cgtcaccgtt gtcgccttct ttctcggcgg
     3661 aacgaaactg tattcggcga tcgaccgcgc ctgcgcccgc attttccaga ctgaccggca
     3721 gcgctacgca aagcgacggc tattcgcgct actgatgatg ccgatgattc caaccctgtt
     3781 gctgatcgcg acatcgctgt cagtcatcgc gacagccgcg ctggcactac caatcgaaga
     3841 ggttgtcgag gtgcagcctt cgacgctgga gtccgtcctc ggctacctgc tctcctacct
     3901 ggtctcgttc ggactgatgc tgatggcgta ctggcggatt ccggagcgtg ggccggggct
     3961 acgctgttct gtcgagggtg cggcggttgg cgcgctgctg atcgttgtgc tggcacagtc
     4021 cttcccgctt tatgttcagc ttaccggagg ctacaacctc tacggaagta tgtttgcctt
     4081 cgtcctgctg ctcctcctct ggctctatct cgtcgggcag atcttcgtga tcggcgcgga
     4141 ggtcgccgcg tttcggtcag gctgtcgtga cgacgtgagt ctgagcgcgt cacaacggag
     4201 cgggcctcgc tgacgaccgg gtcggccgaa tgcacgtgat gatcgcgagg gtacgaccgc
     4261 gaagtgcgac caggagtcgg ttctgttccg caatcgatcg ctcgtatcct gccacttgga
     4321 gtgccctgta gatgttgccg gcgacagatg cagttcggga gcgaagcgtg gcgacgaaga
     4381 gcgtgactga acggcaggcc gttgacgggc agccggtgac cgagaacgag ttgcgtcata
     4441 aaccggtctc gaaggccatc tacggcgtga tcaccgtcct ggccgtcctg caggtcatgg
     4501 aagctcatcc tccgacgggc tggagcggcg ctgtcttcct gttcggcaca accgtcgctg
     4561 ttgcgctggt tgacgcgtac tccgagtcca tcgccatcat gcttgctcag gaacggcgcc
     4621 tctcgctggc cgatctgcgc gcgatccgtc atgaagtggc cccggtcctg atcggcgccc
     4681 aggcaccgac actcattctg ctcgcgtcgg cgttcggtct ggtctccgtc gaactggcga
     4741 tcacgctcgc acaggtcgtc gcgttcgtgc tccttttcag ctacggctgg cgtgttggga
     4801 gcctgctgca cgaacaccgg ctgcgccaga tcctcagcgg gctggttctg gtcgccatcg
     4861 gtgggatcgc tgtaggcatc aaggcggcgt tccactagcg ggaacgtggg taacgcccgc
     4921 gcaggtgagc ttgaaaggca gtctgcggag cacggcggac aaaaccttcc
//
LOCUS       DASUGJ010000354         4754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1166314, whole genome shotgun sequence.
ACCESSION   DASUGJ010000354 DASUGJ010000000
VERSION     DASUGJ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166314"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1654)
                     /locus_tag="VFV93_07865"
     CDS             complement(83..1654)
                     /locus_tag="VFV93_07865"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX5165295.1"
                     /translation="MHVALSPLEFMRRSRRLYPDREAVVDGDLRLSYREFFDRCDQWS
                     SALASLGVRQGDRVAYIAPNTHAQLESFYAVPQLGAVLVPINYRLAASDFSYIINHSG
                     AKIVCAHTDCMAAVDQIRDEIPNVEHFVALEEPRDGWLDYEALLAAASGEFERPEIDE
                     NDLLALNYTSGTTSRPKGVMITHRNAYMNIVGTLIHIHMTPAERYLWTLPMFHANGWT
                     FVWIVTAVGGTHVCLRQVVPQRVFELVHSELITMLCAAPTVLIGLANAPEEQQRLARP
                     GVRVVTAGAPPAAATIGKIEGALGWEIIHAYGLTEVSPLITICEPLPHHADLAPADRA
                     TIKARQGVELLTSGELLVVDDDGNEVPHDGETLGEIVVRGNVVMAGYYNDPDATATAM
                     RGGWFHSGDAAVVHPDGYAEIRDRIKDVIISGGENISSVEVEGVLMGHPAIQEVAVVG
                     LPHERWGESPCAFVVCRPGESVNGDDLRSFARDRLAHFKVPTRYEFVDELPKTATGKI
                     QKFVLRGGQRAAVAQ"
     gene            complement(1780..3432)
                     /locus_tag="VFV93_07870"
     CDS             complement(1780..3432)
                     /locus_tag="VFV93_07870"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5165296.1"
                     /translation="MTNASETDVLIVGAGPVGLTLALDLAWRGQTVTVLELRERSEPP
                     SVKCNHVAARSMEIFRRLGVVQTLRNAGLPEDYPNDIAYRTTMVGRELTRIPIPCRRD
                     RYTATGGPDTWWPTPELPARINQIYLEPILFEHAWTTPGITIHNRVQFDRFRQDNDGV
                     TASAHRLESGEELAFRARYLVGCDGGRSEVRHQIGARLEGDAVVQRVQSSYVRAPQLL
                     SLLTTEPAWGNISLNPRRSGTVYAIDGRETWLIHNYLREDEADFESVDRDWAIRAILG
                     VDERFEYEVISNEDWFGRRLMADHFRDRRVFICGDAAHLWVPYAGYGMNAGLADAGNL
                     AWLLAAQLNGWGDAAILDAYERERLPITEQVSYFAMDHAHAMAKQRKAVPAEVEAETP
                     EGEAVRATIGKAAYDLNVQQYAAAGLNFGYYYDDSPLIAYDGEQHPPYSMGDFTPSTV
                     PGCRTPHIWLADGRSLYDALGPDYTLLRFDLTVDAEPLLAAARTRGVPLELLDVVPAE
                     PAPVYTTALLLSRPDQHVAWRGDQLPDDSLALTDLVRGARRQ"
     gene            3602..4699
                     /locus_tag="VFV93_07875"
     CDS             3602..4699
                     /locus_tag="VFV93_07875"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEX5165297.1"
                     /translation="MEQQTQFVYVGTYTNRPQSSGRGEGIYVYQLDPASGDLTFVSLM
                     PDVPNPSFLTVSADGTRLYAPNETTTLAGRPGGAVSAFAIDRQSGGLALLNRQPSHGT
                     DPCHVRLDQTGRNLLVANYTSGSVAVLPVETDGRLAPASDVRQHHGSGPNPTRQEGPH
                     AHFITPDRSNRFVLVADLGLDQVLIYRLDVDAGTLAAHEPAFAALPPGSGPRHLAFHP
                     SLPILYVINELASTISTCAWDGDGGTLRVLETISTLPKGFDGQNSCAEVRAAPSGRFV
                     YGSNRGHDSIATFAVGPDGSLTPAGHTSTGGENPRNFTLDATGTYLFAANQDSDTIVT
                     FRVDSGTGALTPTGQVTSVPSPVCVLLPLSS"
BASE COUNT          751 a         1584 c         1620 g          799 t
ORIGIN      
        1 agcctcgccc tttgtgaatc gcatggagca gggcgaggct gcggccatcg cccctacgcg
       61 agtgaatccc ggatcctaac ccctactgcg ccaccgccgc gcgctggcca ccgcgcagga
      121 cgaacttctg gatcttgccg gtggcggtct tcggtagctc gtcgacgaac tcgtagcggg
      181 tcggcacctt gaagtgtgcc agccggtcgc gggcgaacga gcgcagatcg tcgccattga
      241 cggactcgcc agggcggcag acgacgaagg cgcaggggga ctcgccccag cgctcgtgtg
      301 gtagaccgac gacggcgact tcctggatgg ctggatggcc catcaggaca ccctcgacct
      361 cgaccgacga gatgttctcg ccgccgctga taatgacatc cttgatccgg tcgcgaatct
      421 cggcgtagcc gtccgggtgg acgacggcgg cgtcgccgct gtgaaaccag ccgccgcgca
      481 tggcggtcgc ggtcgcgtcg gggtcgttgt agtagccggc catcacgacg ttgccacgga
      541 cgacgatctc acccagcgtc tcgccgtcgt gcggcacctc gttaccgtcg tcatcgacga
      601 ccagcaactc gccggacgtc agcagctcga ccccctggcg ggccttgatc gtcgccctgt
      661 cggccggggc gaggtcggcg tggtggggta gtggctcaca gatcgtgatc agcggcgaga
      721 cttcggtcag gccgtaggcg tggatgatct cccagccgag cgcgccctcg atcttgccga
      781 tcgtcgcggc ggccggtggc gcgccggccg tcacgacccg gacacccggc cgcgccagcc
      841 gctgctgctc ctcgggggcg ttcgccagcc cgatcaggac cgtcggcgcg gcgcaaagca
      901 tcgtgatcag ctcgctgtga acgagctcaa agacgcgttg cgggacgacc tgccgcaggc
      961 agacatgcgt gccaccgacg gccgtgacga tccagacaaa ggtccagccg ttggcgtgga
     1021 acatcggcag cgtccagagg tagcgttcgg cgggagtcat gtggatgtgg atcagcgtcc
     1081 cgacgatgtt catgtaggcg ttccgatggg taatcatgac acccttcggc cgcgaggtcg
     1141 tcccgctggt gtagttcagc gccagcagat cgttctcgtc gatctcgggc cgctcgaact
     1201 cgccggaagc cgcggccagc aacgcctcgt agtccagcca gccgtcgcgg ggctcttcga
     1261 gcgcgacgaa gtgctccacg ttcgggatct catcgcggat ctggtcgacg gccgccatgc
     1321 aatcggtgtg ggcgcagacg atcttcgcgc cgctgtggtt gatgatgtac gagaaatcgc
     1381 tggcggccag ccggtagttg atcgggacca gcaccgcgcc aagctgcggc acggcgtaga
     1441 acgactccag ttgcgcatgc gtgttcggcg cgatgtaggc gacccggtcg ccctgccgga
     1501 caccgagcga cgccagcgcc gacgaccact ggtcgcagcg gtcgaagaac tcgcgatagg
     1561 acagccgcag atctccatcg acaaccgctt cgcggtccgg gtagagccgg cgcgagcgtc
     1621 gcatgaattc cagcggggag agtgctacgt gcatgtcaag acctctttcg cgtgggcagg
     1681 cggatgatgc ggtgcatcgt cgcgcaatgg tcctcgttcg gcaaggccaa tagatcaatt
     1741 cgcaggggcg atgtcggtga ctcgtccctg cgaattgatc tattggcggc gcgctccccg
     1801 cacgagatcg gtcagcgcta gcgagtcatc tggcaactga tctccccgcc aggcgacgtg
     1861 ctggtcgggg cgcgagagga ggagcgcggt cgtgtagacc ggcgccggtt cggcggggac
     1921 gacatccagc agttccagcg ggacgccgcg tgttcgggca gccgctagca acggctcagc
     1981 gtcgacggtt aggtcgaagc gcagcagtgt gtagtccggg ccgagcgcgt cgtagagcga
     2041 gcggccatcg gccagccaga tgtgcggcgt ccggcagccg gggacggtcg atggcgtgaa
     2101 gtcgcccatc gagtagggcg ggtgctgctc gccgtcgtag gcgatcagcg gcgagtcgtc
     2161 gtagtagtag ccgaagttga ggccggcggc cgcgtactgc tggacgttga ggtcgtaggc
     2221 ggccttgccg atcgtcgccc ggacggcctc accttccggc gtctcggctt cgacctcggc
     2281 cgggacggcc ttgcgctgct tcgccatcgc gtgggcgtgg tccatcgcga aatacgagac
     2341 ctgctcggtg atcggcagcc gctcccgctc gtaggcgtcg aggatggccg cgtctcccca
     2401 gccgttgagc tgggccgcca gcagccaggc caggttgccc gcgtcggcca gtcccgcgtt
     2461 catcccgtag ccggcgtacg gcacccagag gtgggccgcg tcgccgcaga tgaagacgcg
     2521 tcggtcgcgg aagtggtccg ccatcagccg ccggccgaac cagtcctcgt tggagatcac
     2581 ctcgtactcg aacctctcgt cgacgccgag gatggcccga atcgcccagt cgcgatcgac
     2641 cgactcgaag tcggcctcgt cctcgcgcag gtagttgtgg atcagccacg tctcgcggcc
     2701 gtcgatcgcg tagacggtcc cgctccgccg tgggttgagt gagatgttgc cccaggcagg
     2761 ctcggtcgtc aggagcgaga gcagctgcgg cgcccggacg tagctcgact ggacccgctg
     2821 gacgacggcg tcgccttcca gccgcgcgcc gatctgatgc cgcacctccg agcgcccgcc
     2881 gtcgcagccg accaggtaac gggcgcgaaa ggccagttcc tcgcccgatt cgagccggtg
     2941 ggcgctggcc gtcacgccgt cattgtcctg cctgaagcgg tcgaactgga cccggttgtg
     3001 aatcgtgatg cccggcgtgg tccaggcgtg ctcgaagagg atcggctcca ggtagatctg
     3061 gttgatccgc gccggcagtt ccggcgtcgg ccaccacgtg tcgggcccgc cggtcgccgt
     3121 gtagcggtcg cgtcggcagg ggatcggaat ccgcgtcagc tcccggccga ccatcgtcgt
     3181 ccggtaggcg atgtcgttgg ggtagtcctc cggcagcccg gcattgcgca gcgtctggac
     3241 gacaccgaga cgccggaaga tctccatcga gcgggccgcg acgtggttgc acttgacgct
     3301 gggtggctca ctgcgctcgc gtagctccag gacggtcacc gtctgccccc gccaggccag
     3361 atccagcgcc agcgtcaggc caaccggccc ggctccgacg atcagcacat ccgtctcgct
     3421 cgcgttcgtc atcgctcgtc tccacccttc cgtgcggatg gcaatatcgc gatttggagc
     3481 gtcttgtcaa ccgggcgcct cacggcgcgg gccgatacag gatcggcccc tgcacggttc
     3541 ttgaatcgat gcgggtatca tccacattga tccgtcggcg gatacgcgaa aggcggtcag
     3601 catggaacag cagacccaat tcgtctacgt cgggacctac acgaatcgtc cgcagtcgag
     3661 cggccgtggc gagggtatct acgtctacca gctcgacccg gccagcgggg atctgacgtt
     3721 cgtctcactg atgccggatg tgccgaatcc gtcgttcctg acggtgagcg ctgatggaac
     3781 gaggctctac gcgcccaacg agacgacgac gctggctggc cggccaggtg gcgccgtcag
     3841 cgcgttcgcg atcgaccggc agagcggtgg gcttgcgttg ttgaaccggc aaccgtcgca
     3901 cgggaccgac ccgtgccacg tccggctcga tcagaccggc cgcaacctgc tggttgccaa
     3961 ctacaccagc ggcagcgtcg ccgtcctacc ggtcgagacg gacggacggc tggcgccagc
     4021 gtccgatgta cgccagcatc acgggtccgg cccgaatccg acccgccagg aaggaccgca
     4081 cgcgcacttc atcacgccgg atcgatcgaa tcgcttcgtg ctggtcgccg acctggggct
     4141 ggatcaggtg ctgatctacc ggctggatgt cgacgccgga acgctggcgg cgcacgaacc
     4201 ggccttcgcc gcgctgccgc ccggctccgg gccccgtcac ttggcgtttc atcccagcct
     4261 gccgattctc tacgtgatca acgagctggc ctcaacgatc tcgacctgtg cctgggacgg
     4321 cgacgggggc acgctgaggg tgctggagac gatctcgacg ctgccgaagg gattcgacgg
     4381 tcagaactcg tgcgcggagg tgcgggccgc gccatcgggc cgcttcgtct acggctcgaa
     4441 tcgcggccac gatagcatcg cgaccttcgc ggttggcccc gatgggtcgt tgacgccggc
     4501 tggtcacacg tcaaccggtg gcgagaaccc gcgcaacttc acgctcgacg cgaccggaac
     4561 gtacctgttc gccgccaacc aggacagcga cacgatcgtg acgttccggg tcgattccgg
     4621 caccggcgcg ctgacgccga ccggccaggt caccagcgtt ccatcgccgg tttgcgtgct
     4681 gctccccctg tcgtcctgag ctgcagcgaa ggacctcccc gcgtcagctt cgtatggcgt
     4741 caaagcggag aggt
//
LOCUS       DASUGJ010000355         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_889226, whole genome shotgun sequence.
ACCESSION   DASUGJ010000355 DASUGJ010000000
VERSION     DASUGJ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889226"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(661..1635)
                     /locus_tag="VFV93_07880"
     CDS             complement(661..1635)
                     /locus_tag="VFV93_07880"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX5165298.1"
                     /translation="MTFTIAPRPLTYSHSIGNLALTGRGFSNPYDITVVGGTVLYVLN
                     RSNSNQASQGAVRVTICTVDEQYIGTMAGFGEEDGGLVWPTSIASDSKGNIYIADEHR
                     HDVQVFNREHDFVSKWGSYGSGPSDLNRPSGLAVDSNDNIFVVDHMNYRVQKRRPDGQ
                     VISSWGSAGEGPGEFNLPWGIAVDSQDNVYVADWRNDRIQKFTNDGQYISTIGRSGSG
                     DGELNRPANVAVAADGTIYVADWGNERVQVFSDLGFPITSIIGDSEMSKWGAEFLAAN
                     QDLVAGRKLADMTPEKRFHGPRAIGLDNAGHVIVVDSCRHRLQVYNTI"
     gene            complement(1813..2991)
                     /locus_tag="VFV93_07885"
     CDS             complement(1813..2991)
                     /locus_tag="VFV93_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5165299.1"
                     /translation="MGSDKKKDPILVVLQLGGGNDTINTVVPFADPLYYENRKNVRVN
                     EEDVLKIDDKYGFHPSMAAIKPFWDDGKMAVVNGIGYPDPNYSHFRSMDIWYTAEPEK
                     MATDGWLGKVVRDIDPKAENVLTAVNFGRGLPRALALSGVPVASVAELDSYGLLTSLA
                     SVDQRNSALEVFSCMYDDGFNDDGVLKPIQHANAADPMSQVMSYMGQTGLDAQKGADI
                     LSTAIGKYSSTVEYPKTPIAASLKGIAQVKMADLGTRVFYCAHGSFDTHASQLVTHSL
                     LWKDVSEAVAAFFKDLREHNEADNVVMLLWTEFGRRVKDNGAGTDHGAGGVAFVLGDP
                     VKGGMYGEYPSLAEAELTIGNLKYNNDFRSTYSTILEKWMGVEAKPIVNGSFEQFAFV
                     "
     gene            complement(3098..>3745)
                     /locus_tag="VFV93_07890"
     CDS             complement(3098..>3745)
                     /locus_tag="VFV93_07890"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEX5165300.1"
                     /translation="AQFVCRRLHNYFVSDTLDQAAIDELADVYMKSQYDIRAVMRTLF
                     MSDAFRSRDALYARVKSPAEHVVGIIRLVEDFTYPRWGIRELALETRYMGQDLLNPPS
                     VEGWHTGKEWIDTGILVERVNFAAEQVGDVDKPGIQRLIARLRDMGELSDEAFVDACL
                     DQIGPLEVSERTQDALVNFASKDGPLNLSTGGRAAEERVGEMLQLIVATREFQRV"
BASE COUNT          608 a         1187 c         1267 g          683 t
ORIGIN      
        1 gggctgcgct tagcagcaac gcgaggttgt aggcgaccac gaacagcagc agcatcgtca
       61 ggctggcggt gaaccccgcc aggatcgctc gcgccattcg gtcttgcgtg gtgcgagcgt
      121 gtgccttctc ttcgaccgca acggttggag cgtgcatcag ccgactcctt cgctggatcg
      181 gtgcgccgag cgtaccggtg attgggctgc cgatcgggag atcgttcggg gcacagcgcg
      241 tcagggcggc acacgcttcg gcggggggcg tggcgggaga ggtggtgcgc gtcgtcgcca
      301 cgggcgtcga tgctggttct cgtctctgcg gcgtccggac atcggggtga tccggttcgc
      361 gggctcgtcg gccaacgagc gagttacgag cgtctgagag cgtcgtcatc taccagcgta
      421 cgcctgttgg ccccgccata tcaagacaac gaataagatg ggaggcggga cggggcttga
      481 aagccccgcc tacagtcggc gtcgcgctgc gacgcctcgg gcttcgtcgc ggagcgacga
      541 ttgcatgtag acgtaggttt caatcccacg gacccggcgg cgcgatgcaa tcgccctgcg
      601 cgacgtgacg gggcccgagg gatcaccctc gggccccgtg ggtgcgaatc ggacgactgg
      661 ctagatcgtg ttgtagacct gcagccggtg gcggcagcta tccacgacga tcacgtgccc
      721 ggcgttgtcc agcccgatag cccgagggcc gtggaaccgc ttctcggggg tcatgtcggc
      781 cagcttgcgg ccggcgacca gatcctggtt cgcggccagg aactcggcgc cccacttcga
      841 catctcggaa tcgccgatga tcgaggtgat cgggaagccg aggtccgaga agacctggac
      901 ccgctcgttg ccccagtccg cgacgtaaat cgtgccatca gcagccacgg cgacgttcgc
      961 ggggcgattg agctcgccgt cgccgctgcc ggacctgccg atcgtcgaga tgtactggcc
     1021 gtcgttggtg aacttctgga tccggtcgtt gcgccagtcc gccacgtaga cgttgtcctg
     1081 gctatcgacc gcgatgcccc acggcaggtt gaattcgccg gggccctcgc cggcggagcc
     1141 ccaggacgag atgacctggc cgtccgggcg ccgcttctgg acccgatagt tcatgtgatc
     1201 gacgacgaag atgttgtcgt tgctgtccac ggcgaggccg gacggccggt tcaggtcgct
     1261 cgggccggag ccgtaggagc cccacttcga gacgaagtcg tgctcgcggt tgaacacctg
     1321 cacgtcgtgg cggtgctcgt cggcgatgta gatgttgccc ttactgtcgc tggcgatcga
     1381 ggtcggccag accagcccgc cgtcttcctc gccgaagccg gccatcgtcc cgatgtactg
     1441 ctcgtccacc gtgcagatgg tcacgcgaac cgcgccctgg gaggcctggt tcgagttcga
     1501 gcggttgagg acgtagagga ccgtgccgcc gacgacggtg atgtcgtacg ggttgctgaa
     1561 cccgcgcccg gtcagcgcca ggttgccgat cgagtgggag taggtgagtg gacgcggcgc
     1621 aatagtgaac gtcatgcgcg ctctccgaag gtggagcctt gcaagaaaca gccgggggcc
     1681 gtggactgaa gtccacgtct accatctacc gtcgctacgc gacggggtgt ccggcgtcgc
     1741 gtagcgacac ccagacgata gacgtgggtt tcaacccacg gaacctctgg cgcctctgtg
     1801 gctcctgtct acttacacaa aggcgaactg ctcgaagctg ccgttgacga tcggcttcgc
     1861 ctcgacgccc atccacttct cgaggatcgt ggagtaggtc gagcggaagt cgttgttgta
     1921 cttcaggttg ccgatggtca gctcggcttc agccagcgac gggtactcgc cgtacatgcc
     1981 gcccttgacc ggatcgccca gcacgaacgc gacgccgccg gcgccgtggt cggtacccgc
     2041 gccgttgtcc ttgacgcggc ggccgaactc ggtccagagc agcatcacga cgttgtcagc
     2101 ctcgttgtgc tcgcggagat ccttgaagaa cgcggcaacg gcctcggaga cgtccttcca
     2161 gagcagcgag tgggtcacca gctggctggc gtgggtgtcg aacgagccgt gcgcgcagta
     2221 gaacacacgc gtgccgaggt ccgccatctt gacctgcgcg atgcccttga ggctcgcagc
     2281 gatgggcgtc ttcgggtact cgacggtgga ggagtacttg ccgatcgccg tggacaggat
     2341 gtcggcgccc ttctgggcgt cgaggccggt ctggcccatg taggacatga cctggctcat
     2401 cgggtcggcg gcgttcgcat gctggatcgg cttcagcacg ccatcgtcgt tgaagccgtc
     2461 gtcgtacatg caggagaaca cttcgagagc cgagttgcgc tggtcaacgc tcgccaggct
     2521 ggtgagcaga ccatacgaat cgagctctgc gaccgaggcg accggcacgc cggacagcgc
     2581 cagcgcgcgg ggcaggccgc gaccgaagtt gacggcggtc aggacgttct cggccttcgg
     2641 gtcgatgtcc cggaccacct tgccgagcca gccatcggtg gccatctttt cgggctcggc
     2701 ggtgtaccag atgtccatcg agcggaagtg cgagtagttc gggtccgggt agccgatgcc
     2761 gttgacgacg gccatcttgc catcgtccca gaacggcttg atcgccgcca tgctgggatg
     2821 gaagccgtac ttgtcgtcga tcttcagcac gtcctcttcg ttgacgcgga cgttcttccg
     2881 attctcgtag tacagcgggt cggcgaaggg cacgacggtg ttgatggtgt cgttgccacc
     2941 accaagctgc agcacgacca gaatcggatc cttcttcttg tcggagccca ttccactctc
     3001 cccagccgtt cgcatcgtct acccccacgg taccgacgat tgtgtgaccg gcgactagcg
     3061 cggcggggcg gcgccgcccc gacgcggtgg cggttcccta gacgcgctgg aattcgcgcg
     3121 tcgcgacgat aagctgcagc atctcgccaa cccgctcctc ggcggcgcgg ccaccggtgg
     3181 agaggttcag cgggccgtcc ttggaggcga agttgacgag cgcatcttgc gtccgctcac
     3241 tgacctccag tgggccgatc tggtcgaggc aggcgtccac gaacgcttcg tccgagagct
     3301 cgcccatgtc ccgcagccga gcgatcagcc gctggatgcc gggcttatcc acatcgccga
     3361 cctgctcggc cgcgaagttg acccgctcca ccaggatgcc ggtgtcgatc cactccttgc
     3421 cggtgtgcca cccctcgacg ctcggcgggt tcagcagatc ctgacccatg tagcgcgtct
     3481 caagcgccag ctcgcggatg ccccagcgcg ggtaggtgaa gtcttccacc agccggatga
     3541 tcccgacgac gtgctcggcc gggctcttga cccgagcgta cagcgcatcg cgcgagcgga
     3601 aggcgtccga catgaagagc gtccgcatga cggcgcggat gtcatactgg gacttcatgt
     3661 agacatcggc gagctcgtcg atggcggcct gatccaaggt atcggacacg aagtagttgt
     3721 ggagccgccg gcagacgaac tgggc
//
LOCUS       DASUGJ010000356         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1860367, whole genome shotgun sequence.
ACCESSION   DASUGJ010000356 DASUGJ010000000
VERSION     DASUGJ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860367"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFV93_07895"
     CDS             complement(<1..630)
                     /locus_tag="VFV93_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5165301.1"
                     /translation="MNETIGVFVAWPYANGDLHLGHLAGAYLPPDIFARYHRLRGNRV
                     LMVSGSDSHGTPITVGAEREGLTPREYFEGYHRHFLEAWRDLGISFDLFTHTDTENHF
                     AVSQDIFRTLYANGHIFPETQQQLYCEVDARFLPDRYVEGTCPNCGYTSARGDQCDNC
                     GRTLDAIDLIDPRCKLCGTTPVVRETVHLFFDLPKFEARLLEYINQQEHW"
     gene            934..1083
                     /locus_tag="VFV93_07900"
     CDS             934..1083
                     /locus_tag="VFV93_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165302.1"
                     /translation="MATGIVAAMLLPIWMAAVAVLIAYSAIWVIVRSQHPALELVVIR
                     RDDRR"
     gene            complement(1080..1502)
                     /locus_tag="VFV93_07905"
     CDS             complement(1080..1502)
                     /locus_tag="VFV93_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HEX5165303.1"
                     /translation="MEITFPDGTRVRASGIANRGANDDWRGFGLYCDAAWKPDWEAVT
                     IDWPDFGLPVAPETAASQIVDAFQRAQSGTHVEVGCLGGIGRTGTVLACMAILAGVPG
                     ESAVGWVREHYRPEAVETAAQANWVGWFAAWLERTTAT"
     gene            complement(1526..2299)
                     /locus_tag="VFV93_07910"
     CDS             complement(1526..2299)
                     /locus_tag="VFV93_07910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5165304.1"
                     /translation="MAAVTTIDDLSGLRALAAMLPDTAATVITLHTLRRGSCRAHLTG
                     PIARPTALVVDYDEVPGEPGCFADDPDLLWTILRDMPGWFSANMPESLAPAVGTLIER
                     DWSEPARYLGDLYFTLQRPAPTLSDAAVRRLTPADQPLLDAAPATLRGAGFGTTGGLL
                     AEGIAAAAIVDNALVAIAHTFAISDRHADIGVATHEAFRGRGYATAAASLVAQAIQAS
                     GRTPVWSCGETNQPSLRVAAKLGFQPALRREYVIVESRR"
     gene            2417..3646
                     /gene="ilvA"
                     /locus_tag="VFV93_07915"
     CDS             2417..3646
                     /gene="ilvA"
                     /locus_tag="VFV93_07915"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX5165305.1"
                     /translation="MVSLAEVEAAQDRLRGVIVATPMLPGARLSEDAGGNVFVKAENT
                     QRGGSFKLRGAYNKISGLSAEQRERGVIAASMGNHAQGVALAARMLGAPAIVVMPEEA
                     SLTKITATRRYGAEVILYGQSFDEATAHALALSESEGYTLVHAFDDPAIIAGQGVVGL
                     EILDALPNVGTVIVPIGGGGLIGGIASVISAKRPEARVIGVQAAGCSAVQSSLAAGRP
                     VMLAQARTIADGIAVKRPGEVTLPLIRDLVEEVVTVEEDEIAGAIFYTLQHLRLLVEG
                     AGAVGIAALLAGKVRPRGGEPVCVVLSGGNIDANLLSRVIDQSLARAGRYVVLRTSVP
                     DRPGNLARLAQTVADAGANAVDIHHRRSLWGVPLADTGLELVLEVRDEAHGQKVVATL
                     AAAGYAIHRVGGTEYVE"
BASE COUNT          597 a         1252 c         1368 g          623 t
ORIGIN      
        1 ccagtgctcc tgctggttga tgtactccag cagccgcgcc tcgaacttcg gcaggtcgaa
       61 gaacaggtgc accgtctcgc gaacgaccgg cgtcgttccg cagagcttgc agcgtgggtc
      121 gatcaggtcg atcgcgtcga gtgtgcggcc gcagttgtcg cactggtcgc cacgggcgct
      181 ggtgtagccg cagttcgggc aggtgccctc gacgtagcga tcgggcagga agcgggcgtc
      241 gacctcgcag tagagctgct gctgcgtctc cggaaagata tgcccgttgg cgtagagcgt
      301 gcgaaagatg tcctgcgaga cggcgaagtg gttctcggtg tcggtgtggg tgaacaggtc
      361 gaacgagatg ccgaggtcgc gccaggcttc gaggaagtgc cggtggtaac cctcgaaata
      421 ctctcgcggg gtcagcccct cccgctcggc gccgacggtg atcggcgtcc cgtgcgagtc
      481 gctgccggag accatcagga cgcggttgcc gcgcaggcga tggtagcggg cgaagatgtc
      541 cggcggcagg taggcgccgg ccaggtggcc caggtgcagg tcgccgttgg catacggcca
      601 ggcaacaaaa acgccgatcg tctcgttcat ctgctcacat cgtccttcag agagggccac
      661 ctcgccccgc atcgcgcccg gttggacgcc ggcatcgccc gttcgcgcat ctgcccggca
      721 tctgatgata ccgagtgatg caatccgtcg cgcgctggtg ccggcagggc tggcaagcgt
      781 tttgcattga gagaagcaac attggcttgt tggttgcgct cacgagcgcg tagtctggaa
      841 gacgtgaacg ccatgcgatc cacacaaacg tcctcagcag tcaggggccc ggcaacgcag
      901 gagcttcggc cggccatcgc gaatgtcgga atattggcaa ccgggatcgt cgcagcaatg
      961 ctgctgccga tctggatggc tgcggtcgct gtcctgatcg cctactcggc aatctgggtc
     1021 atcgtccggt cgcaacatcc ggcactggag ctggtcgtca ttcgccgcga cgatcgccgc
     1081 taggtagccg tcgtccgttc gagccaggcg gcgaaccagc cgacccagtt ggcctgggcc
     1141 gctgtctcga ccgcttcggg gcggtagtgc tcccgcaccc agccgacggc tgactcgccc
     1201 ggcacaccag cgagaatcgc catacaggcc agcaccgtcc ccgttcgccc gatcccaccc
     1261 aggcagccga cctccacgtg tgtccccgat tgcgcccgct ggaacgcatc gacgatctgg
     1321 ctcgcggccg tttccggcgc gactggcagg ccgaagtccg gccagtcgat cgtgaccgcc
     1381 tcccaatcgg gcttccaggc cgcatcgcag tagaggccga agccgcgcca gtcgtcgttc
     1441 gcacctcggt tggcgatccc gctcgcgcga acgcgggtcc cgtccggaaa tgtgatttcc
     1501 atcgtcctcc agaagtacgt cggagtcacc gccgtgattc gacgatgacg tattcgcggc
     1561 gcagcgccgg ctggaagccg agcttggcgg cgacgcgcag ggatggctgg tttgtctcgc
     1621 cgcagctcca gacgggcgtc cgtccgctgg cctgaatcgc ctgggcgacg agcgatgcgg
     1681 cggcggtcgc gtagccgcgt ccgcgaaatg cctcatgcgt cgcgacgccg atatcggcgt
     1741 gccggtccga aatcgcgaag gtgtgggcga tggcgaccag tgcgttgtcg acgatcgctg
     1801 ccgcggcgat gccttccgcg agcagcccgc cggtcgtgcc gaaacccgcc ccgcgcaggg
     1861 tggccggcgc ggcgtccagc agtggctggt cggcgggtgt caagcgccgg acggctgcat
     1921 cactgagagt tggcgcgggg cgctgaagcg tgaagtacag gtcgcccagg tagcgggccg
     1981 gttcactcca gtcgcgctcg atcagggttc cgacggcggg cgccagcgac tcgggcatgt
     2041 tggcggagaa ccagccgggc atgtcgcgca ggatcgtcca gagcagatcg gggtcgtcgg
     2101 cgaaacagcc tggctcgccg ggcacctcgt cgtagtcgac gacgagcgcg gtcggacgcg
     2161 caatcgggcc ggtcaggtgc gcgcggcaac tcccgcgacg cagcgtgtgg agcgtgatga
     2221 ccgtcgcggc tgtgtcaggc aacatcgcgg ccagcgcccg caggccggag agatcatcga
     2281 tcgtcgtgac ggcggccatc gcatcacctt tcgtcgtccg gatcgatcta ctgaggtcgt
     2341 ggcgcggact atgatacttg gggcaggagc gtgccggatc agcagccggc aggatacagg
     2401 gataaagggg gagcgcgtgg tcagtctggc ggaggttgag gcagcgcagg atcggttgcg
     2461 cggcgtgatt gtagcgacgc cgatgctgcc gggggcgcgg ctcagcgagg atgctggcgg
     2521 caacgtcttc gtcaaggctg agaacaccca gcgtggcggc tcgttcaagc tgcgcggcgc
     2581 gtacaacaag atctccggat tgtcggccga gcagcgcgag cggggagtga tcgctgcctc
     2641 gatgggcaac cacgcccagg gtgtcgcgct ggccgcgcgg atgctcggcg caccggcgat
     2701 cgtcgtcatg ccggaagaag cctcgctgac caagatcacc gccacccggc gctatggcgc
     2761 ggaggtgatt ctctacggcc agtcgttcga cgaggcgacc gcccatgccc tcgcgctcag
     2821 cgagtctgag ggctacacgc ttgtccacgc cttcgacgac ccggccatca tcgccggtca
     2881 gggagtcgtc ggactcgaaa tcctcgacgc gcttcccaac gtcgggacgg ttatcgtccc
     2941 aatcggcggc ggtggcttga tcggcgggat cgccagcgtc atcagcgcca aacggcccga
     3001 ggcgcgggtg atcggggtgc aggcggccgg ttgctccgcg gtccagtcct cgctggcggc
     3061 cggccggccg gtgatgctgg cccaggcccg gacgatcgcc gatggcatcg ccgtcaagcg
     3121 gcccggcgag gtgacgctgc cgctgatccg cgacctggtc gaggaggtcg tcaccgtcga
     3181 ggaagacgag atcgccggcg cgatcttcta cacgctccag cacctgcggc tgctcgtcga
     3241 gggcgccggc gctgtcggca tcgcggcgct gctggccggc aaggttcgcc cgcgtggagg
     3301 cgagccggtc tgcgtcgtcc tctccggcgg caacatcgac gccaacctgc tctcgcgagt
     3361 catcgaccag tcgctggccc gtgccggccg ctacgtcgtc ctgcgcacgt cagtccccga
     3421 ccggcccggc aatctggccc ggctggccca gacggtcgcc gacgccggcg cgaacgccgt
     3481 cgacattcac caccgccgct cgctctgggg cgtcccgctg gccgacactg gcctggaact
     3541 cgtcctcgaa gtccgcgacg aggcccacgg ccagaaggtc gtcgcgacgc tggctgcggc
     3601 tggctatgcg attcaccgcg ttggcgggac cgagtacgtg gagtgacggg acgcgggacg
     3661 cggataggag cgtgaagttt cgcgtcccgc atcccgcatc ccgccgccct acgtcgcccg
     3721 cagcggcgcg agcgagaagg cggccatgta ggtcgggttg tcgcggcgga aggtgtcggg
     3781 gttgatgccg cgagcggcgg cgatctggta ggccaggatc tggtagggga cgacggccag
//
LOCUS       DASUGJ010000357         5076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1443684, whole genome shotgun sequence.
ACCESSION   DASUGJ010000357 DASUGJ010000000
VERSION     DASUGJ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443684"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1577)
                     /locus_tag="VFV93_07920"
     CDS             complement(51..1577)
                     /locus_tag="VFV93_07920"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX5165306.1"
                     /translation="MANRASGAPPAVRIGFSDEGVTTVAADALVLAVPGSVDSDAAWD
                     GPLAEVDAALHGALREALRDAGFSGTAGKTQVIATLGRLPARRIVVTGLPDDASSADE
                     IRRAYGAAATAAKNAGARNVVCAPPGGALDEAARYRAAVEGLLLALYDFKLYKSDADE
                     NASGIESWSFLTGETDAARRGIDAGYALACGVYLARDLVNEPGQSIYPETLTAAAREM
                     SKENELEFVEFDEHQLVEMGATAIYDVGKGSTHPPRLFHMTYRPSGESKGTIAFVGKG
                     ITFDTGGMNLKPTGGIETMKTDMAGSAAVIGAMRAVAALDLPFTVYGIVASAENMPSG
                     TAFRPGDVVRAMNGKTMEILSTDAEGRLVLSDALVYAAREGADEMIDLATLTGAKVIA
                     IGSESVAVFSNDDDFARRVVAAGTEAGELFWHMPLWDALKKQIRSEIADMKNTGGRPG
                     GAITAALLLAEFAEGKPWVHLDIAGAAWADSARDYTPKGPTGVGVMALVNYLEAKAAG
                     "
     gene            complement(1823..2140)
                     /locus_tag="VFV93_07925"
     CDS             complement(1823..2140)
                     /locus_tag="VFV93_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165307.1"
                     /translation="MLSQVRLRSHSPRTLAPMRCSLGYALHSEADIAKCRAVEGPGDC
                     WKAAPSWRVAAPGPEPRTQERTRHTNGVTAATGEATVEMTEVLVEIVEVRVTTEAATV
                     DDN"
     gene            2511..3971
                     /locus_tag="VFV93_07930"
     CDS             2511..3971
                     /locus_tag="VFV93_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5165308.1"
                     /translation="MRNSGSRQRRVLVIGADGLRPDLLDPAVMPNVARLAEGGVRFGD
                     HHAVYPSHTRVNMSALASGTTPGRHGIVANTMIVPGATEDHIIDTSSYQHIDALARAS
                     DGEALHVPTLGELLPDGGRVAVAGNGSGGSNLLWTHKQRSRIINCNTAFGIADLYDLR
                     EKLGEIPEAATPGISSTGYVARAVTDIFLDDPDNRVIVLWINEPDASLHRFGLGAPEV
                     TQAMAAVDAAVGQIVATMERRGIRDAFDIFFISDHGHSTIQAHNTLREYLIQAARDLG
                     RELPPLVTASDYIYATPGTPEPVAAELAPLVEWIQAQPWCDVVLAGGEDLEELPGVLP
                     LRAIWNGALTPRRPLLAVSPRWTDDRNEYGIPGTVAALTTQAALKSSHGSTSPYDMHP
                     LFIANGPSFRAGVVTDLPSGSIDLTPTVLTLLGLPLPDHLDGRVLWEGLAHPAGEPGQ
                     VRTELLTPRVAAAGRAPVLRLERVGTASYLHGSRPG"
     gene            complement(4123..4980)
                     /gene="modA"
                     /locus_tag="VFV93_07935"
     CDS             complement(4123..4980)
                     /gene="modA"
                     /locus_tag="VFV93_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5165309.1"
                     /translation="MTNRRMRWSRMGLALGLVCMLLVTACGDDDDNAPTPAGSRADAP
                     AGLEGNLTVFAAASLTDAFGLVKQILEDANPDLTIEYNFAGSQALATQLGEGAEADVF
                     ASANKTQMAAAQDADRIDGEPVIFVRNRLAIIVPKDNPAGITEPADLANEGVKLVVAN
                     PDVPVGGYTLDTLDKMSADPTFGADFRTKVGANIVSMESNVKQVVTKVQLGEADAGVV
                     YVSDVTPDVRESVAFIEIPEEFNVIAEYPIAAVSDGDSDLAQAFIDFLLSAGGQEALA
                     EWGFTPVNQ"
BASE COUNT          772 a         1756 c         1669 g          879 t
ORIGIN      
        1 cgggtggggt acagggccgg cacaaggcac ggcccctact tctttggcag ctaccccgcc
       61 gccttcgcct ccaggtagtt gaccagcgcc atcacgccga cgccggttgg gcccttgggg
      121 gtgtagtcgc gggccgagtc ggcccaggcg gcgccggcga tgtcgaggtg gacccacggc
      181 ttgccttcgg cgaactcggc cagcagcagt gcggccgtga tcgcgccgcc ggggcgaccg
      241 ccggtgttct tcatgtcggc gatctcgctg cggatctgct tcttgagcgc atcccagagc
      301 ggcatgtgcc agaacagctc gccggcctcg gtcccggccg cgacgacgcg gcgggcgaag
      361 tcgtcatcgt ttgagaagac ggccaccgac tcgctgccga tcgcgatcac cttcgcgccg
      421 gtcagcgtcg ccaggtcgat catctcgtcg gcaccctcgc gggcagcgta gacgagcgcg
      481 tcggagagga cgagccgccc ctcggcgtcg gtcgagagga tctccatcgt cttgccgttc
      541 atcgcgcgga cgacgtcgcc ggggcggaac gcggtcccgc tcggcatgtt ctcggcgctg
      601 gcgacgatgc cgtagacggt gaagggtaga tcgagagcgg cgacggcccg catcgcgccg
      661 atgacggcgg ccgagccggc catgtcggtc ttcatcgtct cgatcccgcc ggtcggcttg
      721 aggttcatgc ctcccgtgtc gaaggtgatg cccttgccga cgaaggcgat cgtgcccttc
      781 gactcgccac tgggccgata ggtcatgtgg aacaggcgcg gcgggtgggt gctgcccttg
      841 ccgacgtcgt agatggccgt cgcgcccatc tcgaccagtt gatgctcgtc aaactcgacg
      901 aactccagct cgttctcctt cgacatctcg cgcgctgcgg ccgtcagcgt ctccgggtag
      961 atcgactggc ctggctcgtt gacgaggtca cgggcgaggt agacaccgca ggcgagggcg
     1021 tagccggcgt cgatcccgcg ccgggctgcg tccgtctcgc cggtcaggaa cgaccagctc
     1081 tcgatgccgg aagcgttctc gtccgcgtcg gacttgtaga gcttgaagtc gtacagcgcc
     1141 agcagcagcc cttcgacggc cgcccggtag cgggctgcct catccagtgc accgcccggc
     1201 ggcgcgcaga cgacgttgcg tgctcctgcg ttcttcgcgg ccgtcgcggc agcgccatac
     1261 gcgcggcgga tctcgtcggc gctgctggcg tcgtccggca gtccggtcac gacgatccgc
     1321 ctggccggta gccgcccgag cgtcgcgatc acctgggtct tgccggccgt gccgctgaag
     1381 cctgcatcgc gcagcgcctc ccgcaacgcg ccatgtagcg ccgcatcgac ctcggccagc
     1441 ggcccgtccc aggccgcgtc actatcgacg ctgcctggca ccgccagcac cagcgcgtcg
     1501 gccgcgaccg tcgtcactcc ctcgtccgaa aacccgatcc gcaccgctgg cggtgccccg
     1561 cttgccctgt tggccatggt ccctcctcgg tcgtgacgcg tgacgcgtga tgcgtgatgc
     1621 gggtagatcc gcccagcgtt tcaacaccag cgtctcgctg cacctcatcc atttggacgg
     1681 ggagcattgt acggcacgca tcacgcgtca cgcgtcacgc gtcacgcaaa cacaccggcc
     1741 ggcggggata tccccaccgg ccgagtcacg cttcacgcgt tggttccgcg tccgtttggc
     1801 ttgttgtcgt agtaacaggc ggttagttgt cgtcgacggt tgcggcttcg gtcgtgactc
     1861 gtacttccac gatctcgacc aagacctccg tcatctcgac cgttgcctcg ccggtcgcgg
     1921 ccgtgacacc gttggtgtgt cgggtccgct cctgcgtgcg cggctcgggt cccggcgccg
     1981 cgacgcgcca gcttggcgcg gccttccagc aatcccccgg cccctcgacg gcgcgacact
     2041 tcgcgatgtc ggcctccgag tgtaatgcgt agcccagcga gcagcgcatc ggcgcgagtg
     2101 ttctggggct gtgcgatcgc aggcgaacct gcgacaacat cgagcaacca aaccggatct
     2161 cctcacactc gggagtggat tcgaatccgg tgcccgtacg ggcggtttcc agttcggacg
     2221 cggactcaag gcgcgaaggc cgttcatcac gactcatcgg cgcatccatc cgcttcttgt
     2281 tattcggatg gccccaacag ctatccgtcg ctccatgcgg gacggcgcca aacgccgtca
     2341 tcgcatctcc acggcccgcc gcgtcggaga cagggagcgc aacccatcct ggcgaaccgt
     2401 tcgttccatt atagagcatg ttgcttctcg acgtaatgta ggatgtcgcg ggactgcggt
     2461 caggccgtcc cgccgtatcc cggcctcggt gtcagaccaa cggaggacag atgcgcaata
     2521 gtggttctcg gcaacgccgc gtgctggtca tcggtgcgga cgggctacgg ccggacttgc
     2581 tcgacccggc cgtgatgccg aacgtggcga ggctggccga gggtggtgtg cgctttggcg
     2641 accatcacgc cgtctacccg tcgcacacgc gggtcaacat gagcgcgctg gcgtcgggaa
     2701 cgacgccggg gcggcacggg atcgtcgcca acacgatgat cgtgccgggc gcgaccgagg
     2761 accacatcat cgatacgtcc agctaccagc acatcgacgc gctggcccgc gcctcggacg
     2821 gcgaggcgtt gcacgtcccg acgttgggtg agctgctgcc ggacggtggc cgggtggcgg
     2881 ttgccgggaa tggcagcggc ggctcgaacc tgctttggac gcacaagcag cgcagccgga
     2941 tcatcaactg caacacggcc ttcggcatcg ccgacctcta cgacctgcgt gagaagctgg
     3001 gcgagattcc cgaggctgcc acacctggca tcagcagcac cggctacgtc gcccgggccg
     3061 tgaccgacat cttcctcgac gaccccgaca accgcgtcat cgtcctttgg atcaacgagc
     3121 cggacgccag cctgcaccgc ttcggcctcg gcgcgccgga ggtgacccag gcgatggcgg
     3181 ctgttgatgc cgccgtcggc cagatcgtcg cgacgatgga gcggcgaggt atccgcgatg
     3241 cgttcgacat cttcttcatt tccgaccacg gccactcgac cattcaggcg cacaacacgt
     3301 tgcgcgagta cctgatccag gccgcccgcg acctgggccg cgaactaccg ccgctcgtga
     3361 ccgccagcga ctacatctac gccacacctg gcacgcccga gccagtggcc gccgagctgg
     3421 cgccgctagt cgagtggatt caggcgcagc cgtggtgcga cgtcgttctg gccggcggtg
     3481 aggatctgga ggagttaccg ggcgtcctcc cgttgcgggc catctggaac ggcgcgctga
     3541 cgccgcgcag gccgctgctg gccgtctcgc cgcgctggac agatgaccgc aacgagtacg
     3601 gcatccccgg tacggtcgcc gcgctgacga cccaggcggc gctcaagtcc tcacacggct
     3661 caacctcgcc gtacgacatg cacccgctct tcatcgccaa cggccccagc ttccgtgccg
     3721 gcgtcgtcac cgacctcccc agcggctcga tcgacctgac gccgaccgtc ctgacgctgc
     3781 tcggcctgcc gctgcccgat cacctcgacg gccgcgtcct ctgggagggg ctggcgcatc
     3841 cggccggcga gccgggccag gtccggaccg aactgctgac accgcgtgtg gctgctgccg
     3901 gccgcgcgcc agtgctacgg ctcgaacggg tcggtacggc gagctacctc cacggatcgc
     3961 gaccgggctg atctcttcag atttccgcgc ggagcttacc ggtaggggcg aggcactgac
     4021 tcgctcggct cgccgcccga acggtgcggg tggggcacga agggcgagtc agtgccttgc
     4081 ccctaccggg aggtatgccc acggcggtaa cgcgaaaccg ggttattggt ttaccggcgt
     4141 gaagccccat tcggcgaggg cttcctgccc gccggcggag agcaggaagt cgatgaacgc
     4201 ctgggccaga tcgctgtcgc catcgctgac ggcggcaatc gggtactcgg cgatgacgtt
     4261 gaactcttcg gggatctcga tgaacgccac gctctcgcga acgtccggcg tcacgtccga
     4321 gacgtagacg acgccggcgt cggcctcgcc aagctggacc ttggtgacaa cctgtttgac
     4381 gttgctctcc atcgagacga tgttggcccc gaccttcgtg cggaagtcgg ccccgaaggt
     4441 tgggtcagcg ctcatcttgt cgagagtgtc gagcgtgtag ccgccgactg gcacgtcggg
     4501 gttggcgacg accagcttca caccctcgtt ggccagatca gccggctctg tgattccggc
     4561 cgggttgtcc tttggaacga tgatcgccag ccggttgcgg acgaagatga ccggctcgcc
     4621 atcgatccga tccgcgtcct gggcggccgc catctgggtc ttgttggcgg aagcgaagac
     4681 atccgcctcg gcgccctcgc caagctgcgt tgccagtgcc tgggaaccgg cgaagttgta
     4741 ctcgatcgtc aggtcgggat tggcgtcctc aaggatctgc ttgaccagcc cgaacgcgtc
     4801 ggtcagcgat gcggcggcga agacggtcag gttgccctcc aggccagccg gcgcatccgc
     4861 tctgcttccc gccggggtgg gggcgttgtc gtcatcatcg ccacaggccg tcaccagcag
     4921 catgcagacc agccccagcg caagccccat ccgcgaccag cgcatccgtc ggttcgtcat
     4981 cggagaccct tccggtttgg attactcaac cgctgagtat tcgtgtagca cagtagcgaa
     5041 ggatgatggc agcgtcaagc aggtacgaca tgtacc
//
LOCUS       DASUGJ010000358         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1724586, whole genome shotgun sequence.
ACCESSION   DASUGJ010000358 DASUGJ010000000
VERSION     DASUGJ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724586"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFV93_07940"
     CDS             <1..744
                     /locus_tag="VFV93_07940"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5165310.1"
                     /translation="LVISRTAQVDGLFAAASGHILTRQQRERYQISTDDVFVDIGASS
                     RDEAVEMGVHVGAGVIWNPPARKLGTRIYGKAIDDRVALALMTLLLDGLDVATLTYDL
                     YFAATIQEEIGLGGALSLRHDVDADLAIALDNGLVGDIPTVATRNMPVVLGGGPTLVY
                     KDAGIHYDVRIIDHLTDIAEREGIPVQHAVYEQIGSDGSALIKQGIPTALLGPSTRYT
                     HSAFEMIDERDVDHSLRLLRAFVTTDPPA"
     gene            935..2281
                     /locus_tag="VFV93_07945"
     CDS             935..2281
                     /locus_tag="VFV93_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5165311.1"
                     /translation="MRFLRRALPVLVLLTSIAALPSALSAATPAGPAVEICEDLYGDT
                     FGTPNSEPLGACQWDMAIINAGEDGSYGVATGAGVKVGVIDSGVDLTHPDIAPNLDLA
                     LSCSFIFSGTPTAQPQEIANGDCSNKAAIQDLAGHGTHVASTIAAPINGIGIAGVAPE
                     ATIVALKACTMAGYCFADSVAAALRYAGDQRLDVVNLSLFADPYLYYCKSDAQQRAIL
                     KDIEKAAKYAQQRGVLIVASAGNESADLQHPGIDPLSPDWPPDSAEVRDVGNNCRVAP
                     AELPGVLTVSSTGPIGYPDYDLWIATYSSVGMSRVDVAAPGGDYFIATGTVQDAILAA
                     APANSGIYLSLDPLNVRFPGITVVDQGATYMYINGTSMSSPHAAGVAALVVQMHPNWS
                     PGAVKSAVERSAQHLDCPPNWEPLFSGDERTRCYGNGGRTSFFGHGLVDAEAAAEQ"
     gene            complement(2456..2983)
                     /locus_tag="VFV93_07950"
     CDS             complement(2456..2983)
                     /locus_tag="VFV93_07950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5165312.1"
                     /translation="MNEQRDVSYDLEVLDRRHNRPDFSCGVESLDRYFRRFARQDMQR
                     DLAVVYVLNDEGANRVAGYFTLCASSILSDELPASLADRLPSYGAFPAILLGRLAVDR
                     DYQSRNVGRFLLVAAIRHTYRASRSIAAMAVTVDAIDESAGAFYRRFGFISYPSRPLS
                     LFLPMSMAPDLIDGH"
     gene            complement(2967..>3288)
                     /locus_tag="VFV93_07955"
     CDS             complement(2967..>3288)
                     /locus_tag="VFV93_07955"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEX5165313.1"
                     /translation="CHVCTGGSMSSVRDNVETRQLVLEVTQAEFEVIERAAELTRCSV
                     NDFAVGWLRDAAMEVIDSRPDGRLTVEDTIALLEAMDNPPAPSEELRQAFRDYQEFVR
                     ERTT"
BASE COUNT          645 a         1080 c          996 g          567 t
ORIGIN      
        1 ctcgtcatca gccggactgc ccaggtcgac ggactcttcg cggctgccag cggccatatc
       61 ctgacccgcc agcagcgtga gcgctaccag atcagcaccg acgatgtctt cgtcgatatc
      121 ggcgcatcct cacgcgatga ggctgtcgag atgggcgtcc acgtcggcgc gggcgtgatc
      181 tggaacccgc cggcccgcaa actcggcacg cgcatctacg gcaaggcgat cgacgatcgg
      241 gtcgcgctgg cgctgatgac actgctgctc gacgggctcg acgtcgcgac actgacctat
      301 gacctctact tcgcggcaac gatccaggag gagatcggcc tgggcggcgc gctgtcgctg
      361 cgccacgatg tcgatgccga tctggcgatc gcgctcgaca acggcctcgt cggcgatatc
      421 ccgaccgtcg cgacgcggaa catgccggtt gtcctcggcg gcgggccgac gttggtctac
      481 aaggacgccg gcatccacta tgacgttcgc atcatcgacc acctgactga catcgccgag
      541 cgcgagggca tcccggtcca gcatgccgtc tacgagcaga tcggcagcga cggctcggcc
      601 ctcatcaagc agggcatccc gaccgccctg ctcggcccgt caacgcgcta cacccactcc
      661 gccttcgaga tgatcgacga gcgcgatgtc gaccactcgc tgcggctgct gcgcgcgttc
      721 gtgaccaccg atccaccggc atgaccgatc gcgcataacg gtggccgatt cgtgtccatt
      781 ccaggaaagg tcttgaccaa gtcgttgcgg cactctattc tcgtccaacc tggaagagcg
      841 ctagccggga acacctccta caactgaatc tgcaggacga ttcccggcac gtgtaccagc
      901 ggatattcgg ggagtcacgg atggagggaa gccgatgcgg ttcttacggc gagcgttacc
      961 cgtcctggtc ctgttgacct cgatcgcggc gctaccgagc gcactatccg cagcgacgcc
     1021 ggccggacca gccgtcgaaa tctgcgaaga cctctatggc gacacgttcg gtacgccgaa
     1081 tagtgagcca ctcggagcct gccagtggga catggcgatc atcaacgccg gcgaggacgg
     1141 cagctacggc gtcgcgaccg gggctggagt gaaagtcggg gttatcgact ccggcgttga
     1201 cctgacccat ccggacatcg cgccgaacct cgaccttgcc ctgtcgtgct cgttcatctt
     1261 cagcggcacg ccgaccgccc agccgcagga gatcgcgaac ggcgactgct cgaacaaggc
     1321 ggccatccag gacctggcgg gacacggcac gcacgtcgcg tcgacgatcg ccgccccgat
     1381 caacggcatc ggcatcgccg gagtcgcgcc cgaagcgacg atcgtcgcgc tgaaggcgtg
     1441 cacgatggcg ggatattgtt tcgcggactc ggtcgcggca gcgctgcgct acgccggcga
     1501 ccagcggctc gatgtcgtca atctgagcct gttcgccgac ccgtacctgt actactgcaa
     1561 gtccgacgcc caacagcggg cgattctcaa ggatattgag aaggccgcga agtacgcgca
     1621 acagcggggc gtcctgatcg tcgcctcggc cggcaacgag tcggcagacc tgcaacaccc
     1681 gggcatcgac ccgctcagcc cggactggcc accggattca gccgaggtgc gcgatgttgg
     1741 caacaactgc cgcgttgcgc cggccgagct tcccggtgtg ctgactgtct cgtcgaccgg
     1801 accaatcggc tatccggact acgacctgtg gatcgcgacc tactccagcg tcggcatgtc
     1861 ccgcgtcgat gtcgccgcgc caggtggcga ctacttcatc gcaactggca ccgtccagga
     1921 cgcgatcctc gcagccgcac cggccaactc cggcatctat ctcagccttg acccgctcaa
     1981 tgtcagattc cccggcatca ccgtcgttga tcagggcgcg acgtacatgt acatcaacgg
     2041 gacgtcgatg tcgtcgccac acgcggccgg cgtcgcggcg ctggttgtcc agatgcaccc
     2101 gaactggagc ccaggcgccg tcaagtctgc cgtcgagcgc tcggcacaac atctcgactg
     2161 cccgcccaac tgggaaccgc tcttctcagg cgacgagcgg acgcgctgct acggcaacgg
     2221 cggacgaacc tcgttcttcg gccacggcct ggtcgacgcc gaggcagccg cggagcagta
     2281 gaggtctcga atccatcgga tagtagaacg agggctggtc gaatcgacca accctcgttc
     2341 ccgtgttcat cggacagtga ggcattcgtt caacgaagtg ttcgccaacg cgaagtcgac
     2401 cactcgctgc ggctgctgcg tgcgttcgtg acgccagcgc cgccgttgat ggagtctaat
     2461 gaccgtcgat aaggtctgga gccatcgaca tcggtagaaa cagactgaga gggcgggacg
     2521 gatacgaaat gaacccgaac cgtcgataga acgcacccgc actttcgtcg atagcatcga
     2581 ccgtgaccgc catcgcagcg atcgaacggc tcgcgcgata ggtgtgcctg atggccgcta
     2641 cgagcaggaa gcgtccgacg tttcgactct ggtagtctcg gtcaacagcc aagcgtccga
     2701 gcagaatcgc ggggaacgct ccatacgagg gcaatcggtc agcaagcgaa gctgggagtt
     2761 cgtcagacaa gatggagctg gcacacagcg tgaagtagcc cgcaacacga ttcgcgcctt
     2821 cgtcattcag gacgtagacg acggcgagat cgcgttgcat atcctggcgc gcgaaccgcc
     2881 gaaagtagcg gtccaacgac tcaacgccgc agctgaaatc tggtcggttg tgacggcgat
     2941 cgaggacttc gagatcgtac gatacgtcac gttgttcgtt cacggacaaa ctcttgataa
     3001 tctcgaaacg cctgtcgaag ctcctcactt ggagcgggag ggttatccat cgcctccagg
     3061 agagcaatag tatcttcgac cgtcagccgc ccatctggcc gagagtcaat cacttccatc
     3121 gctgcgtctc ggagccaacc aacggcgaag tcattgaccg agcaacgggt cagttcagcg
     3181 gcacgctcaa tcacctcgaa ttcagcttgc gtcacctcaa gaacgagctg gcgggtttcg
     3241 acgttatctc ggacgctgct catggagcct cctgtgcaaa catgacat
//
LOCUS       DASUGJ010000359         5834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1862126, whole genome shotgun sequence.
ACCESSION   DASUGJ010000359 DASUGJ010000000
VERSION     DASUGJ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862126"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VFV93_07960"
     CDS             complement(<1..1098)
                     /locus_tag="VFV93_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5165314.1"
                     /translation="MQDRTGPEPSISNDLITLAAAVIVIVGMRELAAVLVPVLTAIFF
                     LFAFLPLVTWLRLKGVSPGWSKVIAIGLAVALLVAIVVLVVVSGSQFIDNLPEYEDQL
                     RERLAGLTSWLEDRGISVSSDDATEALDYSMILDLGVLIVPSIAAAAAATLVSLVVFV
                     YALIDIERTHQRLTLGLGTNDPQLTQVSRMVTIVSRYIAIRAVLGALAAVLDTILLLA
                     LGVEYALFWGFLSFLMSFIPYAGYWIALIPPMLLALATDGWTAAILVFIGYLLINGAV
                     DSLIAPNLMGRGLDIPPAVTVLSIVFWGAILGPMGTILAMPLTVAFKILLLERSPNAR
                     WLALLVSTGDGTAALEAQRAPPLADTDTVAAE"
     gene            complement(1169..3277)
                     /locus_tag="VFV93_07965"
     CDS             complement(1169..3277)
                     /locus_tag="VFV93_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5165315.1"
                     /translation="MFRFLKWAIGVAIVQGLLLILLAWLLSGFVVRNVPAALTAGLVF
                     GIGFAALWPVIYQLSARIHPILFPILSFALTGLMLVLLVDVVNLIYDDAVSIDGIWTG
                     ILVALGLTVGNTVIGALFGLDDDRAYDRYVTRQIRRSTRGVASSTTPGVLFLEIDGLS
                     APILHRAIEGGYMPTLQRWIESGSHQLRVWEPDLSSQTSASQAGILLGSNWGIPAFRW
                     WDKPLGMLMVSSKRTTARALETQLSTGNGLLAAHGASRWNVFSGDATDCLGTFSRIGD
                     KTASGQRSYLAYFTNPYCLARTISLFFIDVVRELFEASRQRATNVLPRVHRGFKYAFI
                     RAGTTVLLQESSRFMLTADMYRGVPVVYNTFFAYDEVAHHSGIDRLDSMKVLRTLDRV
                     MAHFERVAQTTPRPYHLVVLSDHGQSQGATFKQRYGKTLAEVVSELIGSTGSVTQVGS
                     GDEGLGGINSALTEAIQHDSRSMNMLRRATRSNTSNGEVQLGSEADQALSADVQKALQ
                     ASEAVVVASGNLGLISFPRVPERMTYEQLTSTYPGLLKGLAEHPGVSFVMAHTEDEGA
                     VVVGAKGVIYLADGHVAGEDPLRDFGPNAANHLKRTDSFPNAPDILVMSQIDHDTGEV
                     AAFEELVGCHGGLGGPQTQPFVLYPAALKIESDQPIIGAGHLHRVLKSWVDGQSAIVP
                     DGTGQFAATGGASAQQVPAT"
     gene            complement(3335..3625)
                     /locus_tag="VFV93_07970"
     CDS             complement(3335..3625)
                     /locus_tag="VFV93_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165316.1"
                     /translation="MCCAITTLLLLGPRFFGMMWWLISPNRWDRAFDSFFWPLIGLVF
                     APWTTLMYVAVYQGGVEGVDWFWMILAGLADLSSYMGGAYGNRERVPSYARM"
     gene            complement(3721..4089)
                     /locus_tag="VFV93_07975"
     CDS             complement(3721..4089)
                     /locus_tag="VFV93_07975"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX5165317.1"
                     /translation="MVRGVARTAVIAGTATATANAVNRRQASRQQAQYDEQMAEQAAY
                     ETQQAAIDSQQQIDMLQQQMAQMQAQQAQAAMVPQAPAASPAPGGDLIEQLQQLAQLK
                     EAGVLSDAEFEAAKAQLLGG"
     gene            complement(4150..4578)
                     /locus_tag="VFV93_07980"
     CDS             complement(4150..4578)
                     /locus_tag="VFV93_07980"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEX5165318.1"
                     /translation="MTLGPVDFFAVKFPGNQFQGEIVPALKELVDSGTIQIVDLTFAR
                     KDADGNVDLFEMHDLDEESYKLYESFVGDVNGMLSEEDIDLIAGALEPNSSAALMLFE
                     NSWARRFVEGLENAQAEVIISERVPRVVMNQLLAAQEAEV"
     gene            complement(4623..5264)
                     /locus_tag="VFV93_07985"
     CDS             complement(4623..5264)
                     /locus_tag="VFV93_07985"
                     /inference="COORDINATES: protein motif:HMM:NF025255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HEX5165319.1"
                     /translation="MEDFAFGSITLAMGIVFCFLGLRIFGITLPIFGFMAGFILGATG
                     IEALFGDGFLSTISGWAAGIVLGLLLAALSYVFWYAGVLIAAAATGAQLGSGLMGVFG
                     VEDGFFVFLVAAGLALLFAFVALALALPIWTVIFYMAMSGAVAIIAGFLLIVNTFDIE
                     DLEKGSAWASVNASWLWVFVWAALAAVGMFVQARWITDLEFPDQRWSRMQYAT"
     gene            complement(5542..5775)
                     /locus_tag="VFV93_07990"
     CDS             complement(5542..5775)
                     /locus_tag="VFV93_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165320.1"
                     /translation="MTGLEWLLSWTLFMIYITCLFTVCRLTFQKGYTVLGIVGIFLPL
                     LWLIGAMLPAKQGSSYWVSQGIAHQQQMEQYTR"
BASE COUNT         1150 a         1846 c         1857 g          981 t
ORIGIN      
        1 ttcggccgca accgtgtccg tatccgccag cggtggcgcc cgctgcgctt ccagggcggc
       61 ggttccgtcg ccggtgctga ccagcagcgc cagccagcgg gcattggggc tccgttcgag
      121 cagcaggatc ttgaaggcga cggtgagcgg catcgccaga atcgtcccca tcggaccgag
      181 aatcgcgccc cagaagacga tcgacagcac ggtgacggcc ggcggaatgt cgaggccgcg
      241 acccatcagg ttcggcgcga tcaggctatc gaccgcgccg ttgatgagca gatacccgat
      301 gaagacgagg atggccgccg tccagccgtc ggtggcgagg gccagcagca tcggcgggat
      361 cagcgcgatc cagtacccgg cgtaggggat aaagctcatg aggaatgaca ggaagcccca
      421 gaagagcgcg tactcgacgc cgagcgcgag cagcaggatc gtatcgagaa ccgcagcgag
      481 cgccccaagg accgcccgga tcgcgatata gcggctgacg atcgttacca tccgggacac
      541 ttgcgtgagt tgcgggtcat tcgtgcccag gcccagcgtg aggcgttggt gcgtccgctc
      601 gatgtcgatc agggcataga cgaagacgac cagtgagacg agcgtcgccg ccgccgccgc
      661 cgcgatactg gggacgatca ggacgccgag atcgaggatc atgctgtagt ccagcgcttc
      721 ggtcgcatcg tcgctcgaca cgctgattcc ccggtcctcc agccagctcg tcagcccggc
      781 aaggcgttcc cgaagctggt cctcgtactc cggcaggttg tcgatgaact ggcttccgga
      841 gacgacgacg agcacgacga tcgcgaccag cagcgcgacc gccaggccga tcgcgatgac
      901 cttcgaccag cccggcgaaa cgcccttcag ccggagccag gtgacaagcg gaaggaacgc
      961 aaacaagaag aagatcgccg tcaggaccgg gaccagaacc gccgcgagct cgcgcatacc
     1021 gacgatcacg atgacggcag cggccagggt gatcagatca ttcgaaatgg aaggctcagg
     1081 cccggtgcgg tcctgcatgt cgcgcttcct ccgcgggata ctcacccgga ttcccgatcc
     1141 tgtggttggg cgacggtcag ttcacaggtc aggtcgcggg tacctgctgt gctgacgccc
     1201 cacctgttgc cgcgaattgc cctgtaccat cgggaacgat cgctgactgt ccatcaaccc
     1261 aggacttcag cacccggtgc agatggccgg cgccgatgat gggctgatcg ctctcgatct
     1321 tcagcgcggc tggatacagc acgaacggtt gcgtctgcgg accgcccagc ccgccgtgac
     1381 agccgaccag ctcctcgaac gcggcaacct cgccggtgtc gtggtcgatc tggctcatga
     1441 ccagaatgtc cggtgcgttg gggaaactgt cggtccgttt gaggtggttc gcggcgtttg
     1501 ggccaaagtc ccgaagtggg tcttcgcccg cgacatggcc gtcggccagg tagatgacgc
     1561 ccttggcacc gacgacaacg gcgccttcgt cctccgtatg cgccatgacg aagctgacgc
     1621 ccggatgttc agccaggccc ttcagcagac cgggataggt gctggtcaac tgctcgtagg
     1681 tcatccgctc ggggacgcgc gggaaggaga tcaggcccag gttgccggac gcgacgacga
     1741 ccgcctcgga ggcttgcagt gccttctgaa cgtccgcgct cagcgcctgg tcggcctcgc
     1801 tgccgagttg cacctcgcca ttgctcgtgt tcgagcgtgt ggcgcgtcgc agcatgttca
     1861 tcgaccgcga gtcgtgctgg atcgcctcgg tgagtgcgga gttgatcccg ccgagtccct
     1921 catcaccgct cccgacctgg gtgacgctcc cggttgaacc tatcaactcg gagacgacct
     1981 cggcgagcgt cttgccgtag cgctgcttga atgtcgcgcc ctggctctgc ccgtgatcgg
     2041 agagcaccac gaggtggtac ggacgtggcg tggtctgggc gactcgttcg aaatgcgcca
     2101 tcacgcggtc gagggtgcgt aacaccttca tcgagtccag ccggtcgatg ccggagtggt
     2161 gggcgacctc gtcgtacgcg aagaacgtgt tgtagacaac cgggacgcca cgatacatgt
     2221 ccgccgtcag catgaaccgg ctcgactcct gcaagagaac ggtcgtgccg gcccggatga
     2281 acgcgtactt gaacccacga tggacacgcg gcagaacgtt cgtcgcccgc tgccggctgg
     2341 cctcgaagag ttcgcggacg acatcgatga agaacaggct gatcgttcgg gccaggcagt
     2401 acggattcgt gaagtaggcg aggtagctgc gctgcccgct tgcggtcttg tcgccgatgc
     2461 ggctgaacgt gccgaggcag tcggtcgcgt caccggagaa cacgttccag cggctggctc
     2521 cgtgggcggc cagcagccca ttgccggtcg aaagctgcgt ttccaacgcg cgtgcggtcg
     2581 tgcgtttact ggagaccatc agcatgccaa gcggcttgtc ccaccagcgg aaggccggga
     2641 ttccccagtt gctgccaagc aggatgccgg cctggctcgc cgatgtctgt gaagagaggt
     2701 ccggctccca gacgcgaagc tgatgcgagc ctgactcgat ccagcgctgc agggtcggca
     2761 tgtagccgcc ctcgatcgcg cgatgcagga tcggcgcgga gagaccgtcg atctccagga
     2821 agaggacacc aggtgtggta gacgatgcga cgccacgcgt cgaccggcga atctgccggg
     2881 tgacataccg gtcgtaggcc cgatcgtcgt cgagtccgaa gagcgccccg atcaccgtgt
     2941 tgccgacggt cagcccgagc gcgacgagga tgccggtcca gatcccatcg atgctgacgg
     3001 cgtcgtcgta gatcaggttg acgacatcga cgagcaggac gagcatcagg ccagtcagcg
     3061 cgaacgagag gatcggaaac agaatcgggt ggatgcgcgc cgagagctgg tagatcaccg
     3121 gccagagcgc cgcgaatccg atgccgaaga cgagacccgc ggtcagcgcg gcaggcacgt
     3181 tcctgacgac gaagccggac aggagccacg cgagcagaat caggaggaga ccctgaacga
     3241 tcgcaactcc gatggcccac ttcagaaagc gaaacatacg ctctggccgc gccttcgttg
     3301 actgcatcga cgtaccggcg gatcagtcca gggcttacat acgggcatag ctcggcactc
     3361 gttcgcggtt gccgtaggcg ccacccatat acgacgagag atcagccaga ccggcgagga
     3421 tcatccagaa ccagtcaacg ccttcaacgc caccctggta gacggcgacg tacatcaggg
     3481 tcgtccacgg ggcaaacacc agcccgataa gcggccagaa gaaggagtcg aaggcgcgat
     3541 cccagcggtt cgggctgatc agccaccaca tcatgccgaa gaatctcgga ccgaggagca
     3601 acaacgtcgt aatcgcacag cacatggccc actcctgcgt caaacgatcg cacgatggat
     3661 agggagtgcc cggttgtcgg ccggccgggc actcaaggcg ttgatgggtc gtccagccga
     3721 tcagccacca agcagctgtg ccttcgcggc ctcgaactcc gcatcagaca gcacgccggc
     3781 ttccttgagt tgagcgagtt gctggagttg ttcgatcagg tcacctccgg gagccgggga
     3841 tgccgccggt gcctggggga ccattgcggc ctgcgcctgc tgggcctgca tctgggccat
     3901 ctgctgttgc agcatatcga tctgctgctg tgagtcgatc gcagcctgtt gtgtctcgta
     3961 ggcggcctgc tcggccatct gttcgtcgta ctgcgcctgt tgcctgctgg cctggcggcg
     4021 gttgaccgcg tttgcggttg cggtcgcggt gccggcgata actgcggtgc gtgcgactcc
     4081 cctaaccaat ccaggacctc ggcggcccat tccacctcgt cgcatgttgg atgctcctct
     4141 cgtcgctgac taaacttcgg cttcctgcgc tgccagtagt tggttcatga cgacacgcgg
     4201 cacgcgctct gagatgatga cctccgcctg ggcgttctcc agcccttcga cgaagcggcg
     4261 tgcccaactg ttctcgaaca gcatcagcgc ggccgagctg ttcggttcca acgcgccggc
     4321 aatcaggtcg atgtcctctt ccgagagcat cccgttgacg tcgccgacga atgactcgta
     4381 gagcttgtac gactcctcat ccagatcgtg catctcgaac aggtcgacgt tgccgtcggc
     4441 gtccttgcgg gcaaacgtca ggtcgacgat ctggatcgtt ccggagtcaa ccagctcctt
     4501 gagcgccgga acgatttcgc cctggaactg attgccggga aacttgaccg cgaagaaatc
     4561 gacgggtccg agcgtcatgt cctacctcca tgcttcaccg agacaacaaa cggcgtcggt
     4621 catcaggtcg cgtactgcat gcgcgaccag cgctgatcgg ggaattcgag atcggtaatc
     4681 cagcgcgcct ggacgaacat tccgaccgca gccagcgcag cccagacgaa cacccagagc
     4741 catgaggcgt tgactgatgc ccaggcgctt cccttctcga ggtcttcgat atcgaatgta
     4801 ttcacgatga gcaggaaccc ggcgatgatc gcgactgcgc cggacatcgc catgtagaag
     4861 atgaccgtcc aaatcggcag tgccagcgcg agggcgacga aggcaaacag gagcgcgagc
     4921 ccggccgcga cgaggaagac gaagaagccg tcctcaacgc caaacacgcc catcagcccg
     4981 gagccgagtt gtgctccggt cgctgccgcc gcgatcagca cgcccgcgta ccagaacaca
     5041 tacgagagcg ccgccagcag caagcccagc acgattccgg cggcccagcc tgagatcgtg
     5101 ctgagaaatc catcgccgaa gagtgcttcg ataccggttg ccccgaggat gaatccggcc
     5161 atgaaaccga agatcggcag cgtgatgccg aagatacgca gtcccaggaa gcagaaaaca
     5221 atgcccatcg cgagcgtaat cgatccgaac gcaaaatctt ccattgccta actcctcgtt
     5281 gcgcactgtg gcatccggct tgtcgcgcca cgcatcgaat cggccacgcg atctccgggt
     5341 cgagaacagt atctggaacc aggacgccgg gtgggacaac gacaggtcgt attcactggt
     5401 cgcaatcgca gcgggccgaa gggagcagcc gtcacgcccc tcgggcggca caatgctgat
     5461 tcgtcggtca gagtcgatgc gatggctttg cggccgaccg ctgcactgcc ggcacctgag
     5521 tgaccggcaa gagcgtgccg gctagcgcgt gtactgttcc atctgctgct ggtgcgcgat
     5581 tccctgcgat acccagtagg acgagccttg cttggccggc agcatcgcgc cgatcaacca
     5641 gaggagcggc aggaagatcc caacgatgcc gaggaccgtg tagcccttct ggaaggtcag
     5701 acggcagacg gtgaacaggc aggtgatgta gatcatgaac agcgtccacg acagcaacca
     5761 ttcgagaccc gtcatcaggt cactccactt cgcttctcaa cgtgtcgaga tccatgccgc
     5821 cccatcgaga cagc
//
LOCUS       DASUGJ010000360         5502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1862131, whole genome shotgun sequence.
ACCESSION   DASUGJ010000360 DASUGJ010000000
VERSION     DASUGJ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862131"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1133)
                     /locus_tag="VFV93_07995"
     CDS             complement(39..1133)
                     /locus_tag="VFV93_07995"
                     /inference="COORDINATES: protein motif:HMM:NF044385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5165321.1"
                     /translation="MAPIALALQAAGHQVAFATTPDYCRHIAAHGFDCFRIGIDDVER
                     DMMVSGPPDKPNQAASIWVNLFINARATECLADLVTICDAWHPSLIVREISEFGGCLV
                     AERLGIPHVAVQVSGFRPHLHQLIADPLNRLRHSLGLPPDPDLRMLYRHMLLSPMPAS
                     YHQGLDLPETTLAIQNIPFDSDGQELPDWIAQLPERLTVYASLGTAYNRSADVFDAIL
                     SAVRLEPVNLILTVGEGLDPSSFGPQLPHIHIERYIPQSLLFEHCHAVITHGGFGTVT
                     TALAFGLPMVIIPIAADQPANAQRCAKLGVARVVEPDRRTPQTIRDAVGDILTKRDFR
                     ENATRMQAEMLALPRLDIVVRQLERLANER"
     gene            1405..1920
                     /locus_tag="VFV93_08000"
     CDS             1405..1920
                     /locus_tag="VFV93_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEX5165322.1"
                     /translation="MAWLRLEWMAGDGRMRSFYWLIDDELAGCGRPGGGRDNSRQLDL
                     DLGWLREQGIAALLSLTETPLSDDALDRHGIVSLHLPVEDLSPPTPDQLQDALAFIDF
                     HLSQDRPVAVHCLVGQGRTGTVLAARLIRDGASVEEAIAELRTLSPGAISSPSQERAL
                     AEWARTRGWVM"
     gene            complement(2017..2634)
                     /locus_tag="VFV93_08005"
     CDS             complement(2017..2634)
                     /locus_tag="VFV93_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165323.1"
                     /translation="MSDEVLRELVRELEVAFEAVGRDVPAPEGRVRLGEVALRPLMDR
                     QLDAETSASMSVSELLMAIADGEPRVLVHNAIRNPGPPDWDWSLDSFRIGDRGYIALI
                     LEEEGEEPFQPVYGSWEPYESQAALEASFVAVYEAHLDPILFGTHLWLGERVSRELLT
                     RALWAGLEAADSFRWEQLRDYALPLDTPTGRRAVLDYWLANAPEP"
     gene            complement(2807..3538)
                     /locus_tag="VFV93_08010"
     CDS             complement(2807..3538)
                     /locus_tag="VFV93_08010"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5165324.1"
                     /translation="MGDVRAELDNVLAARSVLIVGAHPDDPDAFAAGTVARWTAAGAR
                     VRYLVVTSGDKGVPNDEINDPTRFIAMREEEQRRSAAALGAEEVIFLHHRDGEVMDSL
                     ELRGQIVREIRRAKAELIVTHDPLTRMFRQHPDHRAVGFATLAAAFPSCRLASFYPEQ
                     IAEGLEPHSVHMLLLAGGDPADLDLFIDISDTFEQKIAALSLHESQASAFVGGLRPRM
                     TQRAEAMGAAAGLALAEAFTYVWLD"
     gene            complement(3605..4420)
                     /locus_tag="VFV93_08015"
     CDS             complement(3605..4420)
                     /locus_tag="VFV93_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX5165325.1"
                     /translation="MADETTRQDPSRDQAQAQAELETTEQRPEITPPTIEKRALVVVA
                     HPDDAEFSCGATAAKWARNGWEVSFLIVTDATGGGDDHATDVGPAARKVIADTRKAEQ
                     RAAAEALGVGNVEFLDYPDGRIEPTLELRRDLVRVMRRLRPWVVVCPSPDRRWDPFFI
                     GRHHPDHLAVGVAASAAVYPAVRNAWDFPELLDEGLREWRVRELWVVGAPISNHYVDV
                     SETVDAKIEALRAHQSQLGNHFTEVERSVRRGLAMIGARYGVGAAEEFHRVVM"
     gene            complement(4570..5085)
                     /locus_tag="VFV93_08020"
     CDS             complement(4570..5085)
                     /locus_tag="VFV93_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5165326.1"
                     /translation="MARVNLVPESDLTPGVQCYIGQTADGVLRGLGGHQPEIAERWLA
                     LYLPLVREDGALPVELKELVRFQIAGYYGCDYCQGFIAPLPLERGVTFDKLAGVLTPD
                     DPRAELTDRERAMLRFTLKMATGKGELEDDDFSEIRHYFDEPQLVELGFLAALLIGYG
                     RFTITGFGLVE"
BASE COUNT          939 a         1786 c         1820 g          957 t
ORIGIN      
        1 tcagtgcctc gcccctaccg ggcaagccgt tcggcccgtt atcgctcgtt tgccagccgt
       61 tccagctggc gtaccacgat atccaatctt ggtagagcaa gcatctctgc ctgcattcgt
      121 gtcgcgttct ctcgaaagtc gcgtttcgtc aggatgtctc cgaccgcgtc acggatcgtc
      181 tgcggcgttc gccgatcagg ttcgacgacg cgcgctactc cgagtttggc gcacctttga
      241 gcgttggccg gttgatcggc cgcaatcggg attatcacca tcggcaatcc aaacgcgagt
      301 gctgtcgtca ccgtgccgaa cccgccgtgg gtgatgacgg cgtgacaatg ctcgaagagc
      361 aagctctgcg gaatgtatcg ttcgatatgg atgtgcggta gttgcggtcc aaacgacgag
      421 ggatcgagac cttctccaac tgtcaagatc agattgaccg gttcgagtcg gacagcagac
      481 aggattgcat cgaagacgtc tgctgacctg ttgtacgccg ttccgagaga agcgtagacg
      541 gtcagacgtt caggaagttg cgcgatccag tctggaagct cctgtccatc ggaatcgaaa
      601 ggaatgttct ggatcgcgag cgtcgtttcc ggaagatcga gcccctgatg atatgacgca
      661 ggcattggtg acaagagcat gtggcgatag agcattcgga ggtccggatc aggcggcaag
      721 ccgagcgagt gacgcaggcg attgagagga tcagcgatca actggtgaag atgcggccgg
      781 aacccggaga cttggaccgc gacatgtgga atacctagcc gctccgcaac gaggcagccg
      841 ccaaattcgc tgatctcccg cacgatcagg ctagggtgcc atgcgtcgca gattgtgaca
      901 agatccgcca ggcactcggt cgctcgtgca ttgatgaaca gattgaccca gatggatgca
      961 gcttgatttg gtttatcggg cggcccacta accatcatgt cgcgctcaac gtcatcaatt
     1021 ccgattcgga aacagtcgaa tccgtgtgcg gcgatgtgtc ggcagtagtc gggggttgtc
     1081 gcgaaggcga cctgatgacc agctgcttgc agcgcaaggg caatcggcgc catcggtcgc
     1141 caatgtccgg acccaggttg cgttgtaaac aatacccgca tgaatcaccg ttctgagccg
     1201 agtacgcagg ttcaggaaag cgtttcagcc tgcccgaacc gtcaggtccg cgacctcgcc
     1261 aaagttccga tgcgttgatg ccatgactgc cgtaggctcg gtacagcaaa ttgctggagt
     1321 aacgatggat gaaccgcggt gcgttgattc tacagtccgg gagcttaggt gagcaatcgc
     1381 tggcgtacgc tcgtggacag ggccgtggcg tggctgcggt tggagtggat ggcgggcgac
     1441 gggcggatgc gcagcttcta ctggttgatc gatgacgagc tggccggttg tgggcggccg
     1501 ggtggtgggc gcgacaactc cagacagctt gacctcgacc tcggctggtt gcgcgagcag
     1561 gggatcgccg cgctgctgtc gctgaccgag acgccactct ccgacgatgc gctggaccgg
     1621 catgggatcg tctcgctgca cctgccggtc gaagatctct caccgccgac ccccgaccag
     1681 ctgcaggatg cgctcgcctt catcgacttc cacctcagcc aggaccggcc cgtcgccgtc
     1741 cactgcctcg tcggccaggg gcggaccggt accgtcctcg ccgcccgcct gatccgcgac
     1801 ggcgccagcg tcgaagaggc catcgccgaa ttgcgcaccc tctcccccgg cgcaatcagc
     1861 agccccagcc aggaacgcgc cctggcggaa tgggcacgga cgcggggctg ggtgatgtga
     1921 cggcgggatt cgggagtggg caactgatcg gcccatcttg aatttgggcg gtcaattacc
     1981 cccgaatccc gaatcccgag tcccgaatcc cgccacctac ggctccggcg cgttcgccag
     2041 ccagtagtcc agcacagccc ggcggccagt cggggtgtcg agcgggaggg cgtagtcgcg
     2101 cagctgttcc cagcggaagc tgtcggcggc ttcgaggccg gcccagaggg cgcgggtcag
     2161 cagctcgcgg ctgacccgct cgccgagcca gaggtgggtg ccgaacagga tcgggtcgag
     2221 gtgggcttcg tagacggcga cgaaggaggc ttcgagtgcc gcctgactct catacggctc
     2281 ccagctgccg tagaccggct ggaacggctc ctcgccttcc tcctccagga tcagcgcgat
     2341 gtagccccga tcgccgatcc ggaagctgtc cagcgaccag tcccagtcgg gcgggccagg
     2401 attgcggatc gcgttgtgga ccagcacgcg cggctcgcca tcggcaatcg ccatcagcag
     2461 ctcggagacg ctcatcgatg cgctcgtttc cgcatccagc tgccggtcca tcaacggccg
     2521 aagagccacc tcacccagcc gaacccggcc ctccggcgcg ggaacgtccc gtcccaccgc
     2581 ttcaaacgcc acctccagtt cccgcacaag ctcacgcaac acctcgtccg acatccgcac
     2641 ccctctcagc ccgatcgtgt ggttcacacc gaccatgctg agcggtgaac gggaagggag
     2701 tggttcacgt ttgtgacggg gtgtcacggt tgcgagggat tcggggttcg ggattcgggg
     2761 ctgaaatgcg ccgaggccca tccccgaatc ccgaatcccg aatccctcaa tccagccaga
     2821 cgtacgtaaa cgcttcggcc agggccagcc ccgcggcggc gcccattgct tcggcgcgtt
     2881 gggtcatccg gggacggagg ccgccgacga aggcggatgc ctggctctcg tggaggctga
     2941 gggcggcgat cttctgctcg aaagtgtcgc tgatgtcgat gaacaggtcc aggtcggcgg
     3001 ggtcgccacc ggccagcagc agcatgtgga cgctgtgcgg ctccagcccc tcggcgatct
     3061 gctcggggta gaagctggcc agccggcagg aggggaaggc ggctgccagc gtcgcgaagc
     3121 cgaccgcgcg gtggtcggga tgctggcgga acatgcgtgt cagcggatcg tgggtgacga
     3181 tcagctccgc cttcgcgcgg cggatctcgc gcacgatctg gccgcgtagc tccagcgagt
     3241 ccatgacctc gccgtcgcgg tggtgcagga agatgacctc ctcggcgcca agcgcggcgg
     3301 ctgagcggcg ctgctcctcc tcacgcatgg cgatgaagcg ggtcgggtcg ttgatctcat
     3361 cgttggggac gcccttgtcg cccgatgtta cgaccaggta gcgcacccgc gcgccggccg
     3421 ctgtccagcg ggcgaccgtc ccggccgcga acgcatccgg gtcgtccgga tgcgcgccga
     3481 cgatcaggac gctcctggcg gccagcacat tgtcgagttc tgccctcacg tcacccatag
     3541 ggtacccggc ccctctccca aacccccgca acctgtcacc cgaaccccga cacctgaccg
     3601 ccatctacat cacgacgcgg tggaactcct cggcggcgcc gacgccgtag cgggcgccga
     3661 tcatcgccag gccccgccgg acggagcgtt cgacctccgt gaagtggttg ccgagttgcg
     3721 actggtgggc gcgcagggct tcgatcttgg cgtcgaccgt ctcgctgaca tcgacataat
     3781 ggttggagat cggcgcgccg acaacccaca gctcgcggac gcgccactcg cgcagcccct
     3841 cgtcgagcaa ctccgggaag tcccaggcgt tgcgaacggc cgggtagacg gcggccgagg
     3901 cggccacacc gacggccagg tggtcggggt ggtggcggcc gatgaagaac gggtcccagc
     3961 gccgatccgg gctgggacag acgacgaccc acggccgcag ccggcgcatg acgcgcacca
     4021 gatcgcggcg tagctccagc gtcggctcga tccggccatc ggggtagtcg aggaactcga
     4081 cgttgccgac ccccagcgcc tcggcggcgg cgcgctgctc ggccttgcgc gtgtcggcga
     4141 tcaccttgcg ggcggccggg ccgacgtcgg tcgcgtggtc gtcgccgccg ccggtcgcgt
     4201 cggtcacgat caggaacgag acctcccagc cattgcgggc ccacttggcg gccgtcgcgc
     4261 cgcaggagaa ttcggcgtcg tcgggatggg cgacgacgac cagtgcgcgc ttctcgatcg
     4321 tgggtggggt gatttcggga cgctgctcgg tcgtttccag ctccgcctgg gcctgtgcct
     4381 gatcgcgact cgggtcctgc cgcgtggttt cgtcagccaa tgctcttcct tcctccctca
     4441 ttccgcgctg atgatactgc cgaatcacgt catcgtccgt gccggacttg gccggtccgg
     4501 catggcgtaa cgatgagtga tcgctgcttc tgtcagaacc ggagacaccg gctgcctatt
     4561 ccgagccggc tactcgacca gcccgaagcc ggtgatcgtg aagcggccgt agccgatcag
     4621 cagcgcggcc agaaagccca gttcgaccag ttgcggctcg tcgaagtagt ggcggatctc
     4681 gctgaagtcg tcgtcctcca gttcgccctt gccggtcgcc atcttgagcg tgaagcgcag
     4741 catcgcccgc tcgcggtcgg tcagctcggc tcgcgggtcg tcgggcgtca ggacgccggc
     4801 caacttgtcg aacgtgacac cccgctccag cggcagcggc gcaatgaacc cctggcagta
     4861 gtcgcagccg tagtagccgg cgatctgaaa gcggacgagc tccttcagct cgaccggcaa
     4921 cgccccatct tcgcggacga gcggcaggta gagcgccagc cagcgctcgg cgatctccgg
     4981 ctggtggccg cccagcccgc gcaagacgcc gtcggccgtc tgaccgatgt agcactggac
     5041 gccgggcgtg aggtccgact cgggaaccag attcacacgc gccatcaaaa tcctcccctg
     5101 agaagctggg tggaacaacg atcgatcggc ccggcgctca tcgcacggcc cgctggtgag
     5161 gatactcgac gcgtcacgat gcaggcgcct gggaaccgag attgacgggt ggtgtcccct
     5221 gtggtgacga cgacgcgatg ctgatcggag tagtgatgcg gtccagcgag ggcttgttcc
     5281 gtaccgacgc cgcgaatcgc gtcagcgcca tcgcttgacg ggcggagtga ctttcgccat
     5341 catgcacgta tccacgtttc gccagtcagg actggatacg gtcgatggcg tcggcagcgg
     5401 atggtttacc cacactcccg gcaccgctca cgccgctgat cggccgcgag cgggaaatcg
     5461 cggaggtctg cgggctgctg ggccgcgccg atgttcgcct gt
//
LOCUS       DASUGJ010000361         5491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1862223, whole genome shotgun sequence.
ACCESSION   DASUGJ010000361 DASUGJ010000000
VERSION     DASUGJ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862223"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFV93_08025"
     CDS             complement(<1..560)
                     /locus_tag="VFV93_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX5165327.1"
                     /translation="MYIIVVGGGKVGYYLTKTLVHEGYEVLLIEKNPAKCEIYTERFG
                     AVVMNGDGAEASTLAAAGAGRADVVISVTGHDEDNLVICQLARHRFKVKRTIARVNNP
                     KNEDLFRRLGVDVTVNPTNLILSLIEQQIPDRHFVHLLALRHAKLAVVEASVAPESSI
                     SGRLVSDVRLPPETSLIAILRGSDVI"
     gene            complement(601..1005)
                     /locus_tag="VFV93_08030"
     CDS             complement(601..1005)
                     /locus_tag="VFV93_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX5165328.1"
                     /translation="MNVVIVGCGRVGARIAATLDQAGHRVSVVDNNPAAFRRLPQDFS
                     GKAIVGMGIDEDVLKAADIEEADAFFAVTNGDNTNIMTAQIAQTVFDIQHVVARIYDP
                     VREDTYRRMGIHTVCPTTTIASLMLSAIQGAS"
     gene            complement(1043..1480)
                     /locus_tag="VFV93_08035"
     CDS             complement(1043..1480)
                     /locus_tag="VFV93_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5165329.1"
                     /translation="MPYRRILVPVHGNDGDYRALELAGMLADKRGADVTLVYVVEVKQ
                     SLPLDADMPEAVAVGESALERAEDYSRSKAEYRLQKLTPELLQARAAGPAIVDEAIER
                     DVDIIVMASRNRQHFGQVTVGDTVPYILKNAPCEVLLERAGFE"
     gene            complement(1540..2274)
                     /locus_tag="VFV93_08040"
     CDS             complement(1540..2274)
                     /locus_tag="VFV93_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5165330.1"
                     /translation="MSRILIFSDIHANLVALEAVLEAAGEVTEVWNLGDVVGYGPKPR
                     ECVERVIGLNPTASLIGNHDWAAIGRLALDEFNPVARFATYWTTAHLGADHMNYLESL
                     PNRVIEDDWMLVHGSPRHPVWEYVYTARVAHQNFEFFDTQVCFLGHTHIQLYISEEMA
                     ARGVPPIHPSDGDVLDMASTRYIVNPGSVGQPRDNDPTAAFAIYEPGLRRVTFRRVVY
                     DIARTQAQMEEAGLPRPLITRLSLGV"
     gene            complement(2274..4319)
                     /gene="mnmG"
                     /locus_tag="VFV93_08045"
     CDS             complement(2274..4319)
                     /gene="mnmG"
                     /locus_tag="VFV93_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX5165331.1"
                     /translation="MTDATLNRQIPTYDVIVIGAGHAGCEAALAAARAGASTLILTPN
                     LDRIGYMPCNPSIGGPAKGHIVAEIDALGGEMAKAIDRTALQIRLLNASKGPAVQALR
                     AQADKTLYCMVMKEALESQPGLELRQESAVRVNVRAVNGSPRVTSVETDFGGVYHCGA
                     AVITAGTFLRGSMIAGEWRSAGGRSGEGADTNLAMSIGDVGIRLRRLKTGTPPRIDAR
                     TIDFDLTELQPGSERPLWFSLAGRSGQLERVELPPIVIYPGQTDGWRRQLACFHVETN
                     ETGHQLIVDNVDRSPMYNGSINGIGPRYCPSIEDKVMRFRHKTSHGLFLEPEGWRTTE
                     VYVQGANTSLPQDVQLAFLRTIPALRNARITRYGYAVEYDAIEPTELTPAFASKRVDG
                     LFLAGQVNGTSGYEEAAGQGLLAGINAARFVAGADPLVLRRDQAYIGVMADDLTTQEF
                     IEPYRMLTSRAEHRLHLRADNADQRLADIAHAAGLIDDCRHRRIANERHAVSELLNAL
                     DAFHVSPNAATNDALGRFDLPTVSRSMTALDYARRPGITLRNLLGALTPLRADLARFA
                     TLDEQILSQATIEATYHAYVEKQQQQIDRARRMEVAAIPSDFDYTALPGLRNEAREKL
                     ISTRPVTVGQASRIAGVTPSDIAVLLVHVRRLGSGAMMPQSIGEARDRLADDQATVG"
     gene            4475..4687
                     /locus_tag="VFV93_08050"
     CDS             4475..4687
                     /locus_tag="VFV93_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165332.1"
                     /translation="MSFMRGVVESAIRNMSPEERQSALREVTGQVVALMDDDERVEAL
                     AAVVRELVSSLPPERIADALANVRSG"
     gene            4717..5412
                     /locus_tag="VFV93_08055"
     CDS             4717..5412
                     /locus_tag="VFV93_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165333.1"
                     /translation="MERTSTLSAVEIVGVAAASAAALGSVVVALGRSQASSARERVVE
                     LPQPDAAMRLSRENIERGRDLARQAATAVAASYPQVRESASDLLHRAASTARPQASRV
                     SSGAATTADQVRTTGANVLDRLQEEVLPAASTVLSGLVERAGEARERSAPAASDLKSV
                     AAAKADVALTKSTSAAKDTMATVVWTAAALSLIYFFLLSPERREQIKTFLWGAVDQTL
                     VLVRDFQGYEEDF"
BASE COUNT          951 a         1651 c         1860 g         1029 t
ORIGIN      
        1 ataatgacgt cgctgccgcg caggatggcg atcagcgacg tttcgggcgg cagtcgcacg
       61 tcggaaacga ggcgtccgct gatcgacgac tccggcgcga cgctggcttc gacgacggcc
      121 agcttggcgt gtcgcaacgc cagcaggtgg acgaagtggc ggtcggggat ctgctgctcg
      181 atcaggctga ggatcaagtt ggtcgggttg acggtgacat cgacgcccag ccggcgaaag
      241 aggtcttcgt tcttggggtt gttgacgcgc gcgatcgtcc gcttgacctt gaagcggtgg
      301 cgggcgagct ggcagattac gaggttgtcc tcgtcgtggc cggtcacgga gatgacgaca
      361 tcggctcgac cggccccggc agcggccagc gtcgatgcct cggcgccgtc gccgttcatc
      421 acgaccgcgc cgaagcgctc ggtatagatc tcacacttgg ccgggttctt ctcgatcagt
      481 agcacttcgt agccctcgtg gacgagggtc ttggtgagat aatagccgac cttgccgccc
      541 ccaacgacga tgatatacat gctctcggcc tgttcctttc agagatgaca tcgcgccggt
      601 ctaggatgcg ccctggattg cgctcaacat cagcgacgcg atcgtggttg ttggacagac
      661 cgtgtggata cccatccgtc ggtacgtgtc ttctcgaacc gggtcgtaga tccgcgcgac
      721 gacatgctgg atgtcgaaaa ccgtctgagc gatctgggca gtcatgatgt tggtgttgtc
      781 gccgttggtg acggcgaaaa atgcatccgc ttcctcgatg tcggcggcct tgagtacatc
      841 ctcgtcgatg cccatcccga cgatggcctt gcccgaaaag tcctgcggca ggcgccggaa
      901 cgctgccggg ttgttatcga cgacactgac gcggtggccg gcctgatcga gggtagccgc
      961 aattctggct ccgactcgac cgcagccgac gatgacgacg ttcacgctga ttccaatctg
     1021 attgccgtag ccggacgcgc cactactcga agcccgctcg ctccagcagc acttcacagg
     1081 gcgcgttctt gaggatatat gggacggtat cgccgacggt gacctggccg aagtgctgcc
     1141 ggttgcgcga ggccatcacg atgatgtcga cgtcgcgttc gatcgcctca tcgacgatgg
     1201 ccggtccggc ggcgcgtgcc tgcaggagtt ccggtgtcag cttctggaga cggtattcgg
     1261 ccttgctgcg cgagtagtct tcggcacgct ccagtgcgga ttcgccaacg gcgaccgctt
     1321 ccggcatgtc cgcgtcgagc ggcagcgact gtttgacctc gacgacgtag acgagagtga
     1381 cgtcggcacc gcgcttatcg gccagcatcc cggccagctc cagtgcccga taatcaccat
     1441 cgttgccgtg taccggcacc aggattcgcc tgtatggcac ctgtctctac tccagatcga
     1501 cagggtcggt tgtgaccctt ccgctcaaac agcgcgtaat catactccca atgacaacct
     1561 tgtgatgagt ggacggggca ggccggcttc ttccatctgc gcttgcgttc tggcgatatc
     1621 gtagacaacg cgccggaacg tgacgcggcg caatcccggc tcatagatcg cgaaggcggc
     1681 ggtcgggtcg ttgtcacgtg gctggccgac agagcccgga ttgacgatgt agcgggtgga
     1741 ggccatgtcg aggacgtcgc cgtcacttgg atgaatggga gggacgccac gggccgccat
     1801 ctcttcggag atgtagagct ggatgtgggt gtgaccgaga aagcagacct gggtgtcgaa
     1861 gaactcaaag ttctgatgcg cgaccctggc ggtgtagacg tattcccaga ccggatggcg
     1921 ggggctgcca tgcaccagca tccagtcgtc ctcgatgacc cggttgggca agctttccag
     1981 gtagttcatg tgatccgcgc cgagatgggc ggtcgtccag taggtggcga agcgagcgac
     2041 cgggttgaac tcgtcgagcg cgagccgacc gatcgcggcc cagtcgtggt tgccgatcag
     2101 tgaggctgtc gggttcagcc cgatcacgcg ctccacacat tcacgtggct ttgggccgta
     2161 gccaacgaca tcgccgaggt tccagacctc ggtgacctcg ccggcggctt cgagcaccgc
     2221 ttcgagcgcg accaggttcg cgtgaatatc cgaaaagatc aaaattcttg acatcacccc
     2281 accgtggcct ggtcgtccgc caggcggtct cgcgcttcgc cgatcgactg aggcatcata
     2341 gcgccggagc caagcctgcg cacatgaacc aggagtaccg caatatcgct cggggtcacc
     2401 cctgcgattc gggatgcctg gccgacggtg acgggccggg tgctaatcag tttctcacgg
     2461 gcctcgttgc gcagccccgg tagcgcagtg taatcgaagt cggacgggat tgcggcgact
     2521 tccatgcgtc gcgcccggtc gatttgctgc tgttgtttct cgacataggc atggtacgtg
     2581 gcctcgatcg tcgcctgcga caggatctgc tcgtcaagtg ttgcaaagcg cgcaaggtcc
     2641 gcacggagcg gagtcagcgc ccccaataga ttgcggagtg tgatgcccgg gcggcgggca
     2701 tagtcgaggg ccgtcatgct gcggctgacg gtcggcaggt cgaagcggcc caatgcgtcg
     2761 ttagttgcgg cgttcgggct gacgtgaaac gcatcgagcg cgttcagcag ctcggagacg
     2821 gcgtgacgct cgttcgctat ccgccgatgg cggcagtcgt cgatcagtcc ggcggcgtgg
     2881 gcgatgtcag cgagacgctg gtcggcgttg tcagcccgca ggtgcaggcg gtgctccgcg
     2941 cgggatgtca gcatccgata tggctcgatg aactcctggg tcgtcaggtc gtcggccatg
     3001 acgccgatat aggcctgatc gcggcgcagg acgagcgggt ccgcgccagc gacgaagcga
     3061 gcggcgttga tgccggccaa caagccctga ccggctgcct cttcgtagcc ggatgtgccg
     3121 ttgacctgtc cggccagaaa gagtccatcg acccgttttg aggcgaaagc cggcgtcagt
     3181 tcggtcggct cgatcgcatc gtactcgact gcgtagccgt agcgagtgat gcgggcgttg
     3241 cggagtgccg ggatcgtccg cagaaacgcg agctgcacat cctgcggcag gctcgtgttg
     3301 gcgccctgga cgtagacctc agtcgtccgc cagccctccg gttcgagaaa caggccgtgg
     3361 ctggttttgt ggcgaaagcg catgaccttg tcttcgatcg acgggcaata gcgcgggccg
     3421 atcccattga tcgatccgtt gtacatcggg gagcggtcga cgttgtcgac gatgagttga
     3481 tggcccgttt cgttggtctc cacgtgaaag cacgccagct gccgccgcca gccatcggtc
     3541 tggcccgggt agatcacgat tggaggaagc tcgacccgct cgagttgacc tgagcgaccg
     3601 gccagcgaga accagagcgg cctctcgctg ccgggctgaa gctcggtcag gtcgaagtcg
     3661 atcgtgcggg cgtcgatgcg cggcggcgtg ccggtcttga ggcggcgcag acggatgcca
     3721 acgtcgccga tcgacatcgc cagattggta tcagctccct cgcccgagcg gccaccggcc
     3781 gagcgccatt caccggcgat catgctgccg cgcaggaagg tgccggcggt aatgacggcc
     3841 gcgccacaat ggtagacgcc accgaagtcg gtctcgacgg atgtcacgcg cggcgatccg
     3901 ttgacggccc gaacgttgac gcgaacggct gactcctggc gcagctcaag tccgggctgg
     3961 gattcgagcg cctctttcat gaccatgcag tagagcgtct tgtcggcctg cgcgcggagg
     4021 gcctggacgg ccggaccttt ggacgcgttg agcagccgga tctggagcgc ggtccggtcg
     4081 atcgccttcg ccatctcgcc accgagcgcg tcgatctcgg cgacaatgtg tcccttggcc
     4141 ggcccgccga tcgacggatt gcagggcatg tagccgatcc ggtcgagatt cggcgtcagg
     4201 atcaatgtgc tggctccggc acgggcggcg gccagggcgg cctcacatcc ggcatggccg
     4261 gctccgatca cgatgacatc gtaggttgga atttgtcgat tcagggttgc atctgtcata
     4321 acaatcgcga agtataggtc tcggctttat ccggcggcaa cgcccgtgcg tactgcaaat
     4381 ggatgatgcc gctgtccgca tttcgaccga tttcgaatgc gacgaagcgt tatccgcgcg
     4441 atcatacgat tcaggattca gaatggagtt gtgattgagc ttcatgcgtg gcgtggtcga
     4501 gagcgccatc cgaaacatgt cgccggagga acggcagtcg gcgttgcgcg aggtaaccgg
     4561 gcaggtcgtc gcgttgatgg acgatgacga gcgggtagag gcactggccg ccgttgtcag
     4621 ggaattggtc agcagtcttc caccggagcg aatcgcggac gcgctggcga atgtgcggtc
     4681 cggctagcgt cgatcaggag caggagggaa gcgcagatgg aacgaacgtc gacactcagc
     4741 gccgttgaaa tcgttggagt ggcagccgcg tctgccgccg cgctcggcag cgtcgtcgtc
     4801 gccctggggc gttcacaggc gtcgtcggca cgcgaacgcg tggtcgagct tccacagccc
     4861 gacgcggcga tgcggctctc gcgcgagaac atcgaacgag ggcgagatct ggcgcgacag
     4921 gccgcgaccg ccgttgcggc ttcgtatcca caggtgcgtg agagcgcctc cgacttgctg
     4981 caccgcgccg ccagtacggc ccgcccgcag gcgtcccgtg tcagcagcgg cgcggcgacg
     5041 acggccgatc aggtgcggac gaccggagcg aacgtactcg accggctcca ggaagaggtg
     5101 ctgccggctg cctcgacggt gctatcaggg ttggtcgagc gtgctggtga ggcgcgagag
     5161 cgctctgctc cggccgccag cgatctcaag agcgtggctg ccgcgaaagc cgatgtcgcc
     5221 ctgacgaagt cgaccagcgc cgcgaaggac acgatggcga cggttgtctg gacggcagcg
     5281 gcgctgtcgc tgatctactt cttcctgctc agcccggaac gccgcgaaca gatcaagacg
     5341 ttcctgtggg gcgccgttga ccagacgctg gtgctcgtgc gcgacttcca gggttacgag
     5401 gaagatttct aacaggcgac cagagaacct ccgcgcaggg gcgatggccg cagcctcgtc
     5461 cttcacaacg ccagtgtcgg cgggaagggc g
//
LOCUS       DASUGJ010000362         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_196591, whole genome shotgun sequence.
ACCESSION   DASUGJ010000362 DASUGJ010000000
VERSION     DASUGJ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196591"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..812
                     /locus_tag="VFV93_08060"
     CDS             114..812
                     /locus_tag="VFV93_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX5165334.1"
                     /translation="MPGLVFTAFVIIAATVLSSWDIDPIFALFGGIGLILVPIELGYL
                     AIYAHRTTQSWSPLEAVAYRNRVPGRRLAILTAGLVGWFLLCLIVSIAFLDEWLAEHV
                     FAWMPHALLQFALVEGNDDAITGWAFVAFLIIAFACNGVAGPITEELYFRGHLLPRLE
                     RYGRGAPIINTVLFGLYHAFSPWRYPAISLGFLPITWMAWRKRSVFVSIAAHMTINMI
                     TVVLIAAAALSSGS"
     gene            complement(1242..1850)
                     /locus_tag="VFV93_08065"
     CDS             complement(1242..1850)
                     /locus_tag="VFV93_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165335.1"
                     /translation="MTQEPDRVPTLICLIGPPAVGKMTVGQALCQITGYHLFHGHVVA
                     DVLSPYFPFGTPSFSRLSQNWRRQFFEEARTAGLDVVTTVAWRFDVPGDAESIGSWLR
                     AYADGGRVLCVELVAPLPVRMARNRTPERWQQKNPVWVTDQYLRETDNAHRYDSGGSF
                     PFAYPHLRLDITDLSAEDAADQIVTHFNLPRIDDDNASSPGG"
     gene            complement(1890..>2681)
                     /locus_tag="VFV93_08070"
     CDS             complement(1890..>2681)
                     /locus_tag="VFV93_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165336.1"
                     /translation="VGAVLWSLTVIAIILPMTLGGNGTISPLLLPLVYVGTGLLLATF
                     IVWELRQAEPVVQVRLFLTRTFRAAAASESCMNVTFFPIALALAVFLEDIQDHSTTLA
                     GVVLAIGSASTVFGSIVGGRLADRFGRRRPALFGRCVAVLGTIPLLTMDASTSPAVLG
                     FWLLIMSLGSGLSLAAVQSAAVESAPRRYSGMATGVFSTSANLGGIIGITLTSVALGD
                     TAGLDAFQTIFILYLATSLLGALITTRVEPWPTSDDDAPIGATTR"
BASE COUNT          464 a          828 c          878 g          511 t
ORIGIN      
        1 cgtctttcat gatcaccgta ccacgatcac ggcagccgga aacatcgacg ttgaacgagt
       61 cggccgtcgc attcgatgaa tactccttcg ggcgatcgat catcctgcat ctcgtgcctg
      121 ggctggtctt caccgccttc gtcatcatcg ccgccacggt cttgtcgtcc tgggacatcg
      181 atccaatctt tgccttgttc ggagggatcg gcttgatcct cgtgccgatt gagttgggct
      241 acctggcaat ctacgcgcac cggacaactc aatcctggtc accgctggag gcggtggcgt
      301 atcgcaatcg ggttccgggt cggcgattag caatcttgac ggcaggattg gttggctggt
      361 tcctcctctg cctgatcgtc tcgatcgcgt tcctggacga gtggctcgcc gagcatgtgt
      421 ttgcctggat gccgcatgct ctgctgcagt ttgccctcgt cgaggggaat gacgatgcga
      481 tcaccggttg ggcgttcgtc gccttcctca tcatcgcgtt cgcctgcaac ggcgttgctg
      541 ggccaattac cgaggagctg tatttccgtg gccatctact cccgcgcctg gagcgctacg
      601 gtcgcggcgc gccgatcatc aataccgtgc tgttcgggct ttaccatgca ttctcgccgt
      661 ggcggtatcc ggcgatcagc ctcgggttcc tgccgatcac ctggatggca tggcggaagc
      721 gcagcgtctt tgtatccatc gcggcacaca tgacgatcaa catgatcact gtcgtgttga
      781 tcgccgcggc agcgctttcc tcaggcagct aaccggcgga tttaccaccg tttcaagtcc
      841 ccgcatatgg tgatcgtcag tgcgatctct ggtacggcga gactcagtcg caaggggcga
      901 acggtcccta gaagacgcgg gacgtctgga tacttcgccg acgatcgcga tcaagccatg
      961 tgctttggcg tgcgatccgg cttcgatacc tgcattcccg caaagaaagc acgtcggcga
     1021 ctcagacaat tggcattgtt caccagcccc tccccatcgc gatcggtagg aggcggcaag
     1081 cctgtgatat gagtcgatcg agcaccagcg gtagtggtca tcgtcggctt gtggcgacac
     1141 tggtgtggga atcctcatcg ctggtgggcc acggttcgac gcgggtcgtg atcagggcgt
     1201 cgaaggttgg ccgtgcgtcg agacggtcga catgctcgat ctcaaccgcc tggcgacgat
     1261 gcgttgtcgt catcgattcg cggcagattg aagtgcgtca cgatctggtc tgcggcgtct
     1321 tcggctgaca ggtctgtgat gtccagtcga agatgcgggt atgcgaaggg aaagctgccg
     1381 ccgctgtcgt agcggtgcgc gttgtcagtc tcccgcaggt actggtcggt gacccaaaca
     1441 gggttcttct gctgccagcg ttccggcgtc cggttgcgcg ccatccgcac cggcagcggc
     1501 gctaccagct cgacgcagag gacgcgcccg ccatctgcgt acgccctcag ccagcttccg
     1561 atcgactcag catcccccgg cacgtcgaac cgccaggcga cggtcgtcac cacgtccagg
     1621 ccggcggtac gggcctcctc gaagaactgg cgtcgccagt tctgggacag gcgggagaag
     1681 gatggtgtgc cgaacgggaa gtacggggag aggacatcgg caaccacgtg cccatgaaac
     1741 aggtgatagc cggtgatctg gcacagcgct tggccgacgg tcatcttgcc gaccgctggt
     1801 gggccgatca ggcagatgag tgtcgggacc cggtccggtt cttgtgtcat gctctcgatc
     1861 atcctgtaac cgtccctgtg ggccctggat cagcgcgtag tcgcaccgat cggggcgtcg
     1921 tcgtcgctgg tgggccacgg ttcgacgcgg gtcgtgatca gcgcgccgag cagcgaggtg
     1981 gcgaggtaga ggatgaagat cgtctggaag gcgtccaggc cggcagtgtc gccgagcgca
     2041 acagacgtca gcgtgatgcc gatgatgccg cccaggttgg ccgaggtcga gaagacaccg
     2101 gtcgccatcc cgctgtagcg gcgcggggcc gactcgacgg cggctgactg gaccgccgcc
     2161 agcgacagcc cgctgccgag cgacatgatc agcagccaga atcccaggac ggccgggctg
     2221 gtcgatgcgt ccatcgtcag cagcgggatt gtgccgagaa cggccacaca gcgcccgaag
     2281 agggccggac gccgccgccc gaatcggtcg gccagccgcc cgccgacgat tgagccgaac
     2341 acggttgagg ctgagccgat cgccaggacg acgccggcca gtgtcgtcga gtggtcctgg
     2401 atgtcctcca ggaagacggc caacgccagc gcgatcggga agaaggtgac gttcatgcac
     2461 gactcgctgg cggctgccgc ccggaaggtg cgcgtcagga acagccgcac ctggacgacc
     2521 ggctcggcct ggcgcagctc ccagacgatg aaggtcgcca gcagcaggcc ggtcccgacg
     2581 tagacgagcg gcagcagcag cgggctgatg gttccgttgc ctcccagggt catcggcaga
     2641 atgatcgcga tcaccgtcag cgaccagagg acggccccga c
//
LOCUS       DASUGJ010000363         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1725159, whole genome shotgun sequence.
ACCESSION   DASUGJ010000363 DASUGJ010000000
VERSION     DASUGJ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725159"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VFV93_08075"
     CDS             <1..853
                     /locus_tag="VFV93_08075"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5165337.1"
                     /translation="IASAKVLSEQLTDDIREISNDGHLRVRYSVEPRPIHAEESIFDN
                     PERVAEYEQSAAIANYGYRKVERLAGNVGYIEQIGLHVVTRAAPTAMAAFNLIADTDA
                     LIIDLRANGGGDPRHVALICSYLLDTQPVHLNSFYSREDGSVEQFWSWPWLPGRRYLD
                     KPVWVLTSSRTFSGAEELSYNLKTLGRATLVGETTGGGAHPVNFYQINAYFEISIPFA
                     RAVNPVTGGNWEGTGVTPDIHVPAGDAFDTAYRLALRAVLDRLDSYLPSPRADLEREV
                     REALGDA"
     gene            complement(899..1903)
                     /locus_tag="VFV93_08080"
     CDS             complement(899..1903)
                     /locus_tag="VFV93_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX5165338.1"
                     /translation="MRFQIQERVVTIGGGTGSFTMLSELKNVVAPGNLWSIVTMADSG
                     GSSRKLMDEFGRPLPLGDLRQALVALSRRSRLWGDLFSYRFPDTNEENGGDGPVGGHS
                     LGNLILHALQDLNGGDLIGAIEDAEELLHTAGRVLPVTLDQATLCAELSDGSIVRSES
                     AIDQAWQRTVLPIERVYLDPPVSAAARARKALERADKVLIGPGDLYTSIIPCLLVEDV
                     AEAIRSCDGEIVYICNVMTKYGETNGYLASDFVRQIHRYLGRRVDTVMVNTNGPTRYP
                     QELLERYGASGAEPVEPDIETLRTLVPRVIPGEFASTKTLIRHDAERVLLALWPELAQ
                     "
     gene            2032..2253
                     /locus_tag="VFV93_08085"
     CDS             2032..2253
                     /locus_tag="VFV93_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165339.1"
                     /translation="MQHVQAGQCGLCVHFGERHTQQPQLIQIRTKHEAPEDFTDECGH
                     PQHAPLHLIVTPISGCAGFEQAPEAIQAD"
BASE COUNT          396 a          757 c          744 g          366 t
ORIGIN      
        1 gatcgcgagc gcgaaggtgc tgtccgagca gttgaccgac gacatccgcg agatcagcaa
       61 cgacggccac ctgcgcgtgc gctacagcgt cgagccgcga ccaatccacg ccgaggagtc
      121 gatcttcgac aatcccgagc gggtggccga gtatgagcag agcgccgcga tcgcgaacta
      181 cggctatcgc aaggttgagc ggctggccgg caacgtcggc tacatcgagc agatcgggct
      241 gcacgtcgtc acgagagccg ccccgacggc gatggccgcc ttcaacctga tcgccgacac
      301 tgacgcgctc atcatcgacc tgcgtgccaa cggcggcggc gatccccgtc atgtggcgct
      361 gatctgctcc tacctgctgg atacccagcc ggtccacctc aacagcttct actcgcgcga
      421 ggatggtagt gtcgagcagt tctggagctg gccgtggctg ccgggccggc gctacctcga
      481 caagccggtc tgggtgctga ccagcagccg cacgttctcc ggcgccgagg aactctccta
      541 caacctcaag acactcggcc gggcgacgct ggttggcgag acgaccggcg gcggcgccca
      601 cccggtcaac ttctaccaga tcaacgccta cttcgagatc tcgattccgt tcgcgcgagc
      661 ggtcaacccc gtcaccggcg gcaactggga agggaccggc gtcacgcccg atatccacgt
      721 cccggccggc gacgcgttcg acaccgccta tcgtctcgcc ctgcgcgccg tcctcgaccg
      781 cctcgacagc tacctcccca gcccccgcgc cgacctcgaa cgggaggtgc gggaggcgtt
      841 gggggatgcg tgaatcggga cgcgtcccgc atcccgcgtc ccgcgtccca cgtcccggct
      901 actgcgccaa ctccggccag agcgcgagca ggacgcgttc ggcgtcgtgg cggatgagcg
      961 ttttggtgga ggcgaattcg ccggggatga cgcgtgggac gagtgtgcgt agcgtctcga
     1021 tgtccggctc gaccggttcg gccccgctgg cgccgtagcg ttcgagcagc tcctgcgggt
     1081 agcgggtcgg gccgttcgtg ttgaccatga cggtgtcgac acgccggccg agatagcggt
     1141 gaatctgtcg cacgaagtcg gacgccaggt agccgttcgt ctctccgtac ttggtcatga
     1201 cgttgcagat gtagacgatc tcgccgtcgc aggagcggat cgcctcggca acgtcttcga
     1261 ccagcaggca ggggatgatg ctggtgtaga ggtcacccgg accgatcagt accttgtcag
     1321 cccgctccag tgccttgcgg gcgcgggcgg cggccgagac gggcgggtcg aggtaaacgc
     1381 gctcgatcgg caggaccgtt cgttgccagg cttggtcgat ggccgattcg ctgcggacaa
     1441 ttgacccgtc gctcagctcg gcgcagagcg tcgcctggtc gagcgtgacg ggcaggacgc
     1501 ggccggccgt gtgcagcagc tcctcagcgt cctcgatcgc gccgatcagg tcgccgccgt
     1561 tgagatcctg caacgcgtgc aggatcaggt tgccgagcga gtggccgccg accgggccgt
     1621 cgcctccatt ttcctcattc gtgtcgggaa agcggtagga aaatagatct ccccagaggc
     1681 gtgagcggcg cgagagagcg accagcgcct ggcgcagatc gccgagcggc agcggccggc
     1741 cgaactcgtc catcagcttg cgtgacgagc cgcccgaatc ggccatcgtc acgatcgacc
     1801 agagattgcc gggagcgacg acgttcttca gctcggacaa catcgtgaac gagccagtac
     1861 cgccaccgat cgtgacaact cgctcctgga tttgaaagcg caccgcggcg aacccccttc
     1921 ggtcgtcttc cacagccctg atgataccgc ctgccggatg gccggtggca tcgaggttgc
     1981 aacgatcggc agtgcgcggc aattgattcg ggcgaagcga tggaggtacc catgcagcac
     2041 gttcaagcag gtcaatgtgg cctctgcgtt cacttcgggg agcgccatac gcaacagccg
     2101 caactgatcc agatccgcac gaagcacgag gcgccggaag acttcaccga cgagtgcggc
     2161 cacccgcagc acgctccgct ccacctgatc gtgaccccca tcagcggctg cgcgggcttc
     2221 gagcaggcgc cagaagcgat ccaggcagat tagcgatagc cac
//
LOCUS       DASUGJ010000364         5482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1168721, whole genome shotgun sequence.
ACCESSION   DASUGJ010000364 DASUGJ010000000
VERSION     DASUGJ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168721"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..402
                     /locus_tag="VFV93_08090"
     CDS             193..402
                     /locus_tag="VFV93_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165340.1"
                     /translation="MLVVTTQERFEDDRDEWAAMEEECIDFLPHHRRMWVMEIADDTG
                     LSEFQALMVTLLAESIQRTASEPEF"
     gene            533..2674
                     /locus_tag="VFV93_08095"
     CDS             533..2674
                     /locus_tag="VFV93_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5165341.1"
                     /translation="MAGKDAVLARLTEADVQRIYAGLGVERFGSGGDGHWRVRSPFRD
                     DRHPSLSIRKVDGVWSDKATGEGGSIFDAIMKRRGGTFTDALEEVARLVGLAGSTVGT
                     REQAATAPVVTEQPEQPEQPEEPREKRRPVLPRAIYPYHDENGELLYEVVRVEEEGKK
                     KRFMARRPARQDEESRKGWVWSLGNGRRVLYRLPEILAAEPWEWIFVVEGEKDADRLR
                     QHGLVATTNAFGAGSWEASFAESLANRAVVVLADNDEAGRQHAEAVVASLRGVAHSVR
                     VPKLLGVPPGGDISDWLDAGGDISTLGRLVELTSCEPRDVPPSAGPATDTAAAEGNEA
                     LKPASRARVRRMSEVPPQDVHWLWQDWLPLGKLTLMGGHVGAGKSTLAAGFAALLSRG
                     LPWPGDEERAAAAGELGPAHSIFLLGEDAADDTLRPRLDQLGANPNYVLTLDAIVDDD
                     GRERMFTLRNHLEELEAAIVEYEARLVVLDPLNSYMGSANSNDEGDVREVLTPLLRVA
                     ERAGCAVLGIGHVGKPSAGGRRMPQQMLLGSTAFIAVARSIMMTAKIPETERNVLASV
                     KSNLSRFPAPLEWSRPDNGPLIWHGPATHSIDAILGGIPGTLRPEPREDAEDLLRVLL
                     ADGPVASREVEAAASELGISEFSIKRARKELGVQTRRLGGSNGGWYLMLEDADWDDFV
                     AGQSTPSSEQDDGLTLPSSPDPIPATSREDA"
     gene            3376..4146
                     /locus_tag="VFV93_08100"
     CDS             3376..4146
                     /locus_tag="VFV93_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX5165342.1"
                     /translation="MTDDIELQSANTAAAYQYAVAFHSEFEGRNPYQVSVIFELHAII
                     SKDLYDFAGQERTAIHEVEIGGASITPAPAYLIKSGLVDLCEDVAKRAGADLSFDDAV
                     RLSAELLHRLLVIHPFQDGNGRTARAFHLMALYDLGLLTPPLHIFDYVAYRRSEYLEC
                     LHEADEGDYEPLCEYIRRGLYDTIEQLIANAVSGGPYREFYMRRLNRKAKRFLDRRYR
                     LGLTGTTYERERRHFLRSITRVAQAASLHEGQHRNPEG"
     gene            complement(4159..4284)
                     /locus_tag="VFV93_08105"
     CDS             complement(4159..4284)
                     /locus_tag="VFV93_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165343.1"
                     /translation="MTSATFGNQIEATDRQIDRLVYELYGLSDDEVWISEAATAR"
     gene            complement(4281..>5482)
                     /locus_tag="VFV93_08110"
     CDS             complement(4281..>5482)
                     /locus_tag="VFV93_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165344.1"
                     /translation="DMSGTAGISLCLDQLLLLVGELNDQQGTDTPRDRFRQFLGTSVT
                     SLGTVRDYVDVCLTHKGDQYDRALQDLVNHTGRLMGFEVDFGRYMGVVNDIGHDGLWK
                     ADGYSIVVEVKTTDAFTIKTGTLLGYINSLIDDGRVSSVDTSMGLYVFGKPVAEVGQL
                     EASILQAGHSQRLRIATVDDILSLAELVQMELLTRDEALSLLRPVGVRVASTVQLLKR
                     VAAGAVVPVADSLADPQPVQPASMAITPSDTDTMFLLTPVASEDGVSAEATIRSLLDQ
                     HVYVFGERTTGRKSLKPGDKIAFYESSKGVVARADVASIPENKAVKFAKQNNEYPWSF
                     KVVNVRYFFNDPVILDASVRRQLDAFAGKDSQAAWSWFVQGTRKISAHDFSVLTRSHK
                     ELDSDAS"
BASE COUNT         1062 a         1683 c         1796 g          941 t
ORIGIN      
        1 ctcactcact cgaacgatcc gagggtcgaa atctgtgcca aacggtcccc atccccgcgt
       61 cacgcgtcac gcgtcacgcg tcaccaaacc cccatccaca cgccatttgg cacgaaatcc
      121 cgccctcaca tcgttcgagt gagtgatggg gggtgtcccc gtcgggtacg cgacgcgaaa
      181 ggagcggcga cgatgctggt ggtgacgacg caggagcggt tcgaggacga ccgggacgag
      241 tgggcggcga tggaggagga gtgcatcgac ttcctgcccc atcaccggcg gatgtgggtg
      301 atggagatcg cggatgacac cggcctgagc gagtttcagg cgctgatggt gacgctgctg
      361 gccgagagca tccagcggac cgccagcgaa cccgagttct aggttccccg tcaggttttc
      421 ccccttcaca acgagacaga catcccccgc tgccgggcgg caggcgtcgc caggctggct
      481 gcggcatggg cgcgcccgct ggcccggcgc gggggttgag aggagcagga cgatggcagg
      541 caaggacgca gtgctggcgc ggttgacgga ggcggatgtc cagcggatct acgccgggct
      601 gggggtcgag cgcttcggca gcggcggcga cggtcactgg cgggtgcgct cgccgttccg
      661 ggacgaccgg cacccgtcgc tgtcgatccg caaggtcgac ggcgtctgga gcgacaaggc
      721 gactggcgag ggcggcagca tcttcgacgc gatcatgaag cggcggggcg gcacgttcac
      781 ggacgcactg gaggaggtcg cccggctggt cgggctggca ggctcgactg tcgggacgcg
      841 ggagcaagcc gcgaccgcgc cggttgtgac ggagcagccg gagcagccgg agcagccgga
      901 ggaaccgcgc gagaagcggc ggccggtgct gccgagagcg atctacccgt atcacgatga
      961 gaacggggaa ctgctctacg aggtcgtgcg ggtcgaggag gagggcaaaa agaagcgctt
     1021 catggcgcgg cgtccggcac ggcaagatga ggagagccgc aagggctggg tctggagcct
     1081 gggcaatggg cggcgggtgc tctaccggct gccggagatt ctggcggccg aaccctggga
     1141 gtggatcttc gtcgtcgagg gcgagaagga cgccgaccgg ctgcggcagc acgggctggt
     1201 ggcgacgacc aacgcgttcg gcgccgggtc gtgggaggcc agcttcgcgg aatcgctggc
     1261 gaaccgggct gtcgtcgtgc tggctgacaa cgacgaggcg gggcggcagc acgccgaggc
     1321 cgtcgtggcg tcgttgcgcg gggtcgcgca ttcggtccgt gtcccgaagc tgctcggagt
     1381 gccgccaggc ggcgacatct ccgactggct cgacgccggc ggggatatct cgacgctggg
     1441 gcggctggtg gagttgacca gttgcgagcc gcgggacgtc ccgccgtctg ctggcccagc
     1501 gacggacacg gcagcggcgg agggaaatga agcactcaag cccgccagcc gcgcacgggt
     1561 gcggcggatg tcggaggtgc cgccgcagga tgtccattgg ctctggcagg actggctgcc
     1621 gctcggcaag ctgacgctga tgggcgggca cgtcggggct ggcaaatcga cgctggcagc
     1681 cgggttcgct gcgctgctct cgcgcgggtt gccctggccg ggagatgagg agcgggccgc
     1741 tgcggcgggc gagctgggcc cagcccactc gatcttcctg ctgggcgagg acgccgccga
     1801 cgatacgttg cgtccccggc tcgaccagct cggagccaac ccgaactacg tgctgacgct
     1861 cgatgcgatc gtggacgacg acggccgcga gcggatgttc acgctccgca accatctcga
     1921 agaactggag gcggcgatcg tcgagtacga ggcgcggctg gttgtgctcg acccgctcaa
     1981 ctcgtacatg gggagcgcca acagcaacga cgagggcgat gtccgcgagg tgctgacgcc
     2041 gttgctgcgg gtggcggagc gggctggttg cgcggtgctt ggcatcggac atgtcggcaa
     2101 gccctcggcc ggcgggcgac ggatgccgca acagatgctg ctcggctcga cggccttcat
     2161 cgccgtcgcc cgctcgatca tgatgacggc gaagattccc gagacggagc gcaacgtcct
     2221 ggcgtcggtc aagtcgaacc tctcacggtt cccagcgcca ctggagtgga gccgccccga
     2281 caacggcccg ctgatctggc acggcccggc gacgcacagc atcgacgcca tcctcggtgg
     2341 catccccggt accctccgcc ccgaaccccg cgaagacgcc gaggacctgc tccgagtgct
     2401 gttggctgat ggaccggtcg cgagccgcga ggttgaagca gcagccagcg aacttgggat
     2461 atccgagttc tcgatcaagc gtgccaggaa ggagctaggc gtccagacgc gacggctggg
     2521 cggcagcaac ggcggctggt atctgatgct cgaagatgcg gactgggatg acttcgttgc
     2581 cgggcagagc acaccatcca gcgagcagga cgatgggttg acgcttccgt cgagccctga
     2641 cccgatacca gccacgtccc gcgaggacgc ctgaacgaga caccggtaaa ggggtcgcgc
     2701 ccatagaggc cacgcgcgtc ccctttactt cttcgcccat cgacatggaa gaatctatgt
     2761 atatctctct cttcgtcgct gtaacagcgt ggttcacgag gcaacgtggt aaaggggtcg
     2821 tcggtaccct cagtggttgc gtcccctttg aacgcctcga ttcggctggc tgaggacaga
     2881 ggcatctcct cagcggccca acggcggcca gatcgtcact tgaacgtcta tacgactggg
     2941 aactggtgcg cttcgcccga ccagacgacg ctgtctggca ccgcgaattg gtgccaatgg
     3001 cggggaattc gcggaacgca ccctgcgcta tactgagtcc ggcgaatgtg tcgtcaacgt
     3061 gacaccggcg tcacccagga tattcgccaa agagaggagt ttcgaagtgc cgagatggtt
     3121 cacagcgaag cgcgaccgat cgcggcaacc agcgttgccg gctgacgata cactaccgac
     3181 cgctgcgtcg ttggctgaac tccgggatat gatccagcga cggcagcagc cgagccgtgt
     3241 cgttgccaag gggcgaccac caacggatga agagatcgtg atggcggcat ggggtaacct
     3301 cgctgccgaa gatccaacgg tgacgatcga ggacgtacga cagggcctgg cgcgacggcg
     3361 atcacccgcc acgacatgac cgacgacatc gaactgcaat cagcgaacac ggcggccgcg
     3421 taccagtacg cggtcgcttt tcattcggag tttgaaggtc gcaaccccta tcaggtcagc
     3481 gtcatctttg aactccacgc gatcatctcg aaggatctgt acgactttgc cggacaagag
     3541 cgcaccgcta ttcatgaggt tgagattggc ggcgcgtcga tcacaccagc ccccgcgtac
     3601 ctcatcaaga gcgggttagt cgatctctgc gaagacgttg ccaagcgcgc cggagccgat
     3661 ctctccttcg atgatgcggt ccgcctgtca gcggaacttc tccatcgatt gctggtcatt
     3721 catccgttcc aggatggaaa cggtcggacc gcgcgagcat tccatctgat ggcactctac
     3781 gatctgggcc tgctcacccc accgctgcac atcttcgact acgtcgccta ccgcaggagt
     3841 gagtatctgg agtgcctgca cgaagccgac gaaggcgact acgagccgct ctgcgagtac
     3901 atccgccgtg gactctacga tacgatcgaa caactcattg cgaatgctgt atcgggcggt
     3961 ccctaccgtg agttctatat gcggagactc aacaggaagg ccaagcgttt ccttgaccgc
     4021 cggtaccgcc tcggactcac cggaacaacc tatgaacgag aacggcggca ctttctcagg
     4081 tcaataacca gggtcgccca ggcagcgtcg cttcacgaag gacagcaccg taacccggaa
     4141 ggctgacacg tcagccgcct agcgcgccgt cgccgcctca ctgatccaca cctcgtcgtc
     4201 gctcagcccg tacagctcgt agaccagccg gtcgatctgc cggtcggtcg cctcgatctg
     4261 gttgccgaag gtggctgaag tcatgatgcg tctgagtcaa gttctttgtg gctgcgggtg
     4321 agcacactga agtcgtgagc cgatatcttc cgggtgccct ggacgaacca cgaccacgcc
     4381 gcttgagaat ccttccccgc aaaggcgtcc aattggcgtc tcacggacgc gtccagaatc
     4441 accggatcgt taaagaagta ccgaacgttc acaaccttga acgaccaggg gtactcatta
     4501 ttttgcttgg caaacttgac cgccttgttt tccgggatcg acgcaacgtc agcacgtgca
     4561 acgacgcctt tactcgactc gtaaaacgca atcttgtcgc caggcttcag cgatttccga
     4621 ccggtcgtgc gttcgccgaa gacgtacaca tgctgatcga gcagcgaccg gatcgtcgct
     4681 tccgcgctta caccgtcctc actcgcaact ggtgtgagca gaaacatcgt gtcggtgtcc
     4741 gatggggtaa tggccatcga agcaggctgc accggttgag ggtcagcaag agaatcagca
     4801 accggaacga ctgcgcccgc agcaacccgt ttcaagagtt ggaccgttga cgccacgcga
     4861 acgccaaccg gacgaagcag ggaaagcgct tcatcgcgcg tcagcagttc catctggacg
     4921 agttcagcga gggaaaggat gtcgtcgaca gtggcgattc gcaaccgctg agagtgtccc
     4981 gcctggagaa tcgatgcttc aagttgtccc acctcagcaa caggtttgcc gaagacatac
     5041 agccccatcg atgtatcgac gctgctgaca cgcccatcat cgatgaggga gttgatataa
     5101 ccgaggagag tcccggtctt gattgtgaag gcgtccgtcg tcttcacctc gacaacgatg
     5161 gaatagccgt cggccttcca taatccgtcg tggccaatat cgttgacgac acccatataa
     5221 cgtccaaaat ccacctcaaa ccccatcagc cggccggtgt gattcacgag gtcttgaagt
     5281 gctctgtcat attggtcgcc tttgtgtgtg aggcatacgt ccacgtaatc tcggaccgtc
     5341 ccaagcgacg tgaccgatgt tcccaggaac tggcgaaacc tgtctcgcgg cgtgtctgtc
     5401 ccttgctgat cgttgagctc cccgaccaac agaaggagtt ggtccaaaca aagggagata
     5461 ccagccgttc cagacatgtc cg
//
LOCUS       DASUGJ010000365         3644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1725330, whole genome shotgun sequence.
ACCESSION   DASUGJ010000365 DASUGJ010000000
VERSION     DASUGJ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725330"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFV93_08115"
     CDS             complement(<1..436)
                     /locus_tag="VFV93_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165345.1"
                     /translation="MDTVVTRATLIDRLADSRARVLERAAAGTLDEQALARLADAEAR
                     FALVLRPAPRDPARNLLEAHGLARPYLERLLTHDPAVEVIVNAEGYSYTPRKVLRRVL
                     DHALDHLNQIDQWRRWRETGAVPTPTDGWASSLVTFAEDRVPL"
     gene            563..1276
                     /locus_tag="VFV93_08120"
     CDS             563..1276
                     /locus_tag="VFV93_08120"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5165346.1"
                     /translation="MNDLANLLRVLADESRLELLTTLIDGDATVSELAARTGLPQPRV
                     SSHLAILRAAGLVDSQRSGRQRAYHVDAERITPVLAALEVLLPARVETIPSPPRRSRQ
                     ATALVTHDAPIRRARTCYDHLAGVAGVQLLDQLIARGWLRADGDDGKRTHYALTASGE
                     RELAARGVDLAGARASRRMHAYGCLDWTERRHHLGGALGAAILQSLIDRGVVTRRTDG
                     RDVDVQATLESWLDASPVS"
     gene            1336..1593
                     /locus_tag="VFV93_08125"
     CDS             1336..1593
                     /locus_tag="VFV93_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165347.1"
                     /translation="MTPTEPEPTDDGPEQPAGEQTTPEATETERKLTSDDFLVPGPTP
                     ESNAGMARWGLTWLAFSVILILLMLCVSFVCLTLANWTGFA"
     gene            complement(1599..2006)
                     /locus_tag="VFV93_08130"
     CDS             complement(1599..2006)
                     /locus_tag="VFV93_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5165348.1"
                     /translation="MIDPRTHIRIARPSRDLVAAERFYVDGLELMVLYRASAAGEGEH
                     DLLMLGWREAGWHFELTRDPADPLQPSPTPDDLFVLYLNGPVDASLVERLVAAGGTQV
                     AAHNAYWDRYGVTIEDPDGYRLVLCERAWSNAG"
     gene            2175..>3644
                     /locus_tag="VFV93_08135"
     CDS             2175..>3644
                     /locus_tag="VFV93_08135"
                     /inference="COORDINATES: protein motif:HMM:NF006122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5165349.1"
                     /translation="MTGGQAMVQSLKKHGVDTMFVLPGVQLDNTFDALYDEKDSIKLY
                     HTRHEQATSYMADGYARTTGKPGVCLVVPGPGLLNAMAGLSTAYACSSPVLAISGQIN
                     SDLIGLGRGMLHEINDQLTAVRSVTKWAERIDQPEDASKLVQEAFRQMLSGRPRPVEI
                     EMAPDIMGKVGEVDILGPAAIDKPAPDMDKINQAAKALGNAKKPLIFVGGGIFDATEE
                     LLALAEMLQAPVVMSNNGKGAVDARNYLGVNTLVGRQLWKDTDAVLAVGTRFVQPLTQ
                     WAPDDEMTIIQMDVDPEEVGRNYAPNIGIVSDAKAGLAALVEATGKHNSNRESREQEL
                     NALKQKMDDILYEIQPQASWAEAIRGELPEDGILISESTQVGYWSGMGFPVYKPRTYL
                     TSGYQGTLGYGFATALGAAVGNPQTRTISINGDGGFMYNVQELSTARMFNIPLTTIVF
                     NDNAFGNVKRIQQESYNGRTIASDLFNPDFVKLGESFDIP"
BASE COUNT          658 a         1220 c         1205 g          561 t
ORIGIN      
        1 ccagcgggac gcgatcctca gcgaacgtca cgagcgacga tgcccagccg tcggtgggtg
       61 ttgggaccgc gccggtttcc cgccatctgc gccactgatc gatctggttg agatgatcga
      121 gcgcgtggtc gagcacccgc cgcagcacct tgcgcggggt gtagctgtag ccttccgcat
      181 tgacgatgac ctcaacggcc gggtcgtggg tcagcaatcg ctccagatag ggtcgcgcca
      241 gcccgtgcgc ttcgagcagg ttgcgggccg ggtcgcgcgg cgcgggacgc aggaccagcg
      301 cgaagcgggc ctcggcgtcg gcgaggcgtg ccagcgcctg ctcatcgagc gtgccggccg
      361 ccgcccgttc cagaacgcgc gctcgcgaat cggccagccg gtcgatcaga gtcgctcgcg
      421 tgacaacggt gtccattgcg tgctttcctc tcacgtgacg cgtcgccgcg tcttccatac
      481 atctcatcga cgagatgtgt tgctgcgaca tatactatcg cagagatcta tgccgtgcaa
      541 cctccgagac aggagcatcc cgatgaacga cctggccaac ctgctacgtg tgctcgcgga
      601 cgaatcgcgg ctggagctgt tgacgacgct gatagacggc gacgcgaccg tcagcgagct
      661 ggcggcccgg actggcctgc ctcagccgcg cgtctcgtcg cacctggcga tcctgcgcgc
      721 ggcggggctc gtcgattccc agcgatcggg acggcagcgt gcctaccacg tcgatgccga
      781 gcggatcaca ccggtgctgg cggcgcttga agtcttgctt ccggcgcggg tggagacgat
      841 tccgtctcca ccacgacgca gccgccaggc gacggcgctg gtgacgcacg atgcgccgat
      901 tcgccgggcg cggacctgct acgaccatct ggccggcgtc gccggcgttc agctcctgga
      961 ccagttgatc gcgcgcggct ggctgcgggc cgatggagac gacggtaagc gcacccacta
     1021 cgcgctgacg gccagcggcg agcgggaact ggcagcccgt ggcgtcgatc tggccggcgc
     1081 acgcgcatcc cgcaggatgc acgcgtatgg ctgcctggac tggaccgagc ggcgccatca
     1141 cctcggcggc gcgctcggcg cggccatcct gcagtcgctg atcgacagag gcgtcgtcac
     1201 gcgtcgcacg gatgggcgcg acgtcgacgt tcaggcgacg ttggagagct ggctcgacgc
     1261 gtcgcctgtc tcatagcgcc gcaagatggc aggattgcgc cagttgagcg gcacacagac
     1321 cgaacgacgg agccaatgac accaaccgag ccagaaccga ccgacgacgg gccggagcaa
     1381 ccggccggcg aacagacgac acccgaggca actgagaccg agcgcaagct gacgagcgat
     1441 gatttcctcg tgccggggcc gacaccggag tcgaacgccg gaatggcccg ctgggggctg
     1501 acctggctgg cgttctcggt gatcctgatc ctgctgatgc tgtgcgtctc gttcgtctgc
     1561 ctgacgctgg cgaactggac cggcttcgcc tgaaccgatc agccggcatt gctccaggcg
     1621 cgctcgcaga gcaccagccg gtagccgtcc gggtcctcga tcgtgacgcc gtagcggtcc
     1681 cagtaggcgt tgtgggcggc gacctgcgtc ccaccggccg ccaccagccg ctcgaccagc
     1741 gaggcatcga cgggcccatt caagtagagg acgaacaggt catccggcgt cggggacggc
     1801 tgaagcgggt cggctggatc gcgcgtcagc tcgaagtgcc agccggcctc gcgccagccc
     1861 agcatcaaca gatcgtgctc gccctcgcca gcggccgatg cccggtagag caccatcagc
     1921 tccagcccat cgacgtagaa gcgttcggca gcgaccagat cgcgggacgg ccgcgcgata
     1981 cgaatgtgtg tcctcgggtc gatcatctcg tctcttcttt cttgtcggca actgaccagt
     2041 atcgcgctcg tgccatgcaa gcgcacgtct cgtcagatag actacaatgg catgcggcct
     2101 gacaggagaa gcgggagggg atgactggga ttccccacgc ccatatgcgg gaaagaggag
     2161 gaaccttggc gcagatgacc ggggggcagg cgatggtcca gtccctcaag aaacacggtg
     2221 tcgacacgat gttcgtgctg ccgggcgtcc agctcgacaa caccttcgac gcgctctacg
     2281 acgagaagga ctccattaag ctctatcaca cgcgccacga acaggcgacg tcgtatatgg
     2341 ccgatggcta cgcgcggacg accggcaagc cgggtgtctg tctggtcgtg ccggggccgg
     2401 gcctgctgaa cgcgatggcc ggtctctcga ctgcctacgc ctgctcgtcg ccggtgctgg
     2461 cgatctccgg ccagatcaac tccgacctga tcggccttgg ccgagggatg ctgcacgaga
     2521 tcaacgacca gctgacggcc gtccgctccg tcaccaagtg ggccgagcga atcgatcagc
     2581 cggaagacgc ctcgaagctc gtccaggaag ccttccgcca gatgctctcc ggccggccgc
     2641 gcccggtcga gatcgagatg gcgccggaca tcatgggcaa ggttggcgag gtcgacatcc
     2701 tcggcccggc cgcgatcgac aagcccgcgc cggacatgga caagatcaac caggcggcca
     2761 aggcgctcgg caacgcgaag aagccgctga tcttcgtcgg cggcggcatc ttcgacgcga
     2821 ccgaggaact gctggcgctg gccgagatgc tccaggcgcc ggtcgtcatg tccaacaacg
     2881 gcaagggcgc cgtcgacgcc cgcaactacc tgggcgtcaa cacactggtt ggccgccagc
     2941 tctggaagga caccgacgcc gtgctggctg tcggcacccg tttcgtccag ccactgaccc
     3001 agtgggcgcc ggacgacgag atgacgatta tccagatgga cgttgatccg gaggaagtcg
     3061 gccgcaacta cgcgccgaac atcggcatcg tctccgacgc gaaggcgggg ctggccgcgc
     3121 tggtcgaggc gaccggcaag cacaacagca accgcgaatc ccgcgagcaa gagctcaacg
     3181 ccctcaagca gaagatggac gacatcctct acgagatcca gccgcaggcg tcctgggccg
     3241 aggcgatccg tggcgaactg cccgaagacg gcatcctgat ctccgagtcc acccaggtcg
     3301 gttactggtc ggggatgggc ttcccggtct acaagccgcg cacctacctg acgagtggct
     3361 accagggcac gctcggctac ggcttcgcga cggcgctcgg cgcggccgtc ggcaacccgc
     3421 agacacgtac gatctcgatc aacggcgacg gcggcttcat gtacaacgtc caggagctgt
     3481 cgacggcccg catgttcaac attccgctga cgacgatcgt cttcaacgac aacgcgttcg
     3541 gcaacgtcaa gcgcatccag caggagagct acaacggccg gacgatcgcc agcgacctgt
     3601 tcaacccgga cttcgtcaag ctcggcgagt cgttcgacat cccg
//
LOCUS       DASUGJ010000366         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_891558, whole genome shotgun sequence.
ACCESSION   DASUGJ010000366 DASUGJ010000000
VERSION     DASUGJ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891558"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VFV93_08140"
     CDS             complement(<1..697)
                     /locus_tag="VFV93_08140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5165350.1"
                     /translation="MSGETTGKANSRRSQRTLAPGTVLEDRYEIQKVAGRGGMSTVYV
                     ARDLRFSQVERLCAVKEMFDVDPDARVRALRLVNFERESALLATLSHPAIPRIYDYFS
                     RGGLVYLVLEYIDGEDLERVLANQKMPFQEDQILRWAIEICDVLSMLHDQKPDPVIFR
                     DLKPSNIMLRVSGQIVLIDFGIARTIQGRQRGTMIGTEGYAPPEQYRGIADARGDIYA
                     LGATLHHMATNSDP"
     gene            974..1741
                     /gene="lepB"
                     /locus_tag="VFV93_08145"
     CDS             974..1741
                     /gene="lepB"
                     /locus_tag="VFV93_08145"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5165351.1"
                     /translation="MHDGYSAETVPSNLDGTTRPGQIVHHDTAANANEPPRDTAELPV
                     IEQATSEPQGRSAIWEIVETLLLAVFIFIAVRSVVLNFRVDGLSMEPSLDSGEMLLVN
                     RQIYFHFDTHALVNWLPFVELEGENIVYPFHPPQRGDIVVLHPPVDNGKPYIKRVIGL
                     PGERLGIHDGAVYINGERLHEPYLNGIATAWSGSLGQEEITIPDDEVFVMGDNRNNST
                     DSRIFGPIKIDEIIGKAWISYWPTDRLDILNSPDYSQ"
     gene            1865..>2342
                     /locus_tag="VFV93_08150"
     CDS             1865..>2342
                     /locus_tag="VFV93_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX5165352.1"
                     /translation="MIETIVESIRVSLVTQARVVILKEVEGERHLPIWIGPFEAEAIA
                     MELQSIQSSRPLPYDLLRSVIGELGAEVRRIVITEITNDVFYARIVLDSQGSSVEIDS
                     RPSDAIALAVRVKVPIFVDDSVMERAGVRLDEDEDEDSDEQIRESAPISEERLGVFR"
BASE COUNT          438 a          712 c          738 g          454 t
ORIGIN      
        1 gtgggtccga gttggtcgcc atgtggtgca gtgtcgcgcc gagcgcgtag atgtcaccac
       61 gggcgtcggc aatgccgcga tactgctccg gcggcgcgta cccctcggtc ccgatcatcg
      121 tcccacgctg gcgcccctgg atcgtccggg cgatgccgaa gtcgatcagg acgatctgac
      181 cgctgacacg cagcatgatg ttggatggct tgagatcgcg gaagatgaca gggtcgggct
      241 tctgatcgtg caacatgctc agcacgtcgc agatctcaat cgcccagcgc aggatctgat
      301 cctcctggaa gggcatcttc tggttggcga gcacccgttc gaggtcttcg ccgtcgatgt
      361 actccaggac gagatagacc agcccgccgc gcgagaagta gtcgtagatg cgtgggatgg
      421 ccgggtgcga gagggttgcc agcagcgccg attcgcgttc gaagttgacc agccgtagcg
      481 cccggacgcg ggcgtccggg tcgacatcga acatctcttt gacggcacag agccgctcaa
      541 cctggctgaa acgcaggtcg cgcgcgacgt agaccgtgct catcccgccg cgtccggcga
      601 ccttctggat ctcgtagcga tcctccagaa cggttccggg tgcgagcgtc cgttgcgagc
      661 ggcggctgtt cgccttgccg gtcgtctcac cgctcatcgc ggatgattcc ctgttgatgt
      721 tcgtcgtgcc ggaagatcgg gcgccacgat caccattgac cccacattac agaccacacg
      781 aattggacgg tggctcgaat tctcgccccg gttctgagga tacaggtcga tgctcgcccc
      841 ctcaatcagg agaaagtccc gatttggcgt tgcgaacctg tgataaccat cggcgggtgt
      901 gctcgttgtc cggttgtata ctggaaatca cccggcatca cgccggcgca gtcgttcgga
      961 ggataagaca ctcatgcatg acgggtactc agccgagacg gtgccgagca acctggatgg
     1021 caccacccgt cctggtcaga tcgtccatca cgacaccgcc gcgaacgcca acgagccacc
     1081 acgcgacacc gccgaattgc cggttatcga gcaggcgacc agcgagcccc agggccgcag
     1141 cgcgatctgg gagattgtcg agacgctgct tctggcggtc ttcatcttca tcgcggtccg
     1201 ctcggtcgtt ctcaacttcc gtgtcgatgg cctctcgatg gaaccgtcgc tcgattcggg
     1261 cgagatgttg ctcgtgaacc ggcagatcta cttccacttc gacacacacg cgttagtcaa
     1321 ctggctgccg ttcgtcgagt tggaggggga gaacatcgtc tacccgttcc atcctccgca
     1381 gcgaggtgat atcgtcgttc tccatccgcc ggtcgacaac ggcaagccgt acatcaagcg
     1441 cgtgatcgga ctgccgggcg agcggctggg gattcacgac ggcgccgtct atattaacgg
     1501 cgagcggttg cacgagccgt atctgaacgg gatcgcgacg gcctggtccg gatcgctcgg
     1561 ccaggaagag atcacgattc cggacgacga agtgttcgtg atgggtgata accgtaacaa
     1621 ctcgaccgac tcgcgcatct tcggtccgat taagattgac gagatcattg gcaaagcctg
     1681 gatctcgtac tggccgaccg atcgacttga tattctgaac tccccggact actcgcaatg
     1741 agcgacgcca ttcggggtgg cagggttgat gcgccgaata gcaaattgag tgggcagcag
     1801 cggcagtcct gaggccggat cggtggccag agcatccggt agcagtcccg aagggtagcc
     1861 agccatgatt gagacgattg tcgagagtat ccgcgtcagc ctcgtaactc aggcccgcgt
     1921 cgtcatcctc aaggaagtcg agggcgagcg ccacctgccg atctggatcg gcccgttcga
     1981 ggccgaggcg attgcgatgg agctgcaaag catccagtcc agccggccgt tgccatatga
     2041 cctcctgcgc tccgtgatcg gtgagctcgg cgccgaggtg cgcagaatcg tcatcaccga
     2101 gatcaccaac gatgtcttct acgcccggat cgtgctcgac agccagggtt ccagcgtcga
     2161 gatcgattcg cgtccgtcgg acgcaattgc gctggccgtt cgcgtcaagg tgccgatctt
     2221 cgtcgatgac tcggtcatgg agcgcgccgg tgtccggctc gatgaagacg aggacgagga
     2281 ttccgacgag cagattcgcg agagcgcgcc gatctccgag gagcggctgg gcgtcttccg
     2341 cg
//
LOCUS       DASUGJ010000367         7516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1030684, whole genome shotgun sequence.
ACCESSION   DASUGJ010000367 DASUGJ010000000
VERSION     DASUGJ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030684"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /gene="typA"
                     /locus_tag="VFV93_08155"
     CDS             complement(<1..1811)
                     /gene="typA"
                     /locus_tag="VFV93_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX5165353.1"
                     /translation="MTRPALRNIAIIAHVDHGKTTLVDGLLKQSHIFRDPDAAGELIM
                     DANDLERERGITILSKNASITYNDVKINIIDTPGHADFGGEVERVLNMADGCLLVVDA
                     VEGPMPQTRTVLTRALGIGLRPIVVVNKVDRQFAQPENVIERTQDLFLDLATDADQLD
                     FPIIYAIAREGRAGFAPDDLADDLRPLLDTILSVIPAPVADPAGPTQLQIASLDYDLH
                     RGRIAIGRINRGVIRSGETLVHIDLEGNQTRQKVSWLATHEGLSRREVSEAFAGDIVA
                     LTGFADAKVSATLADPDHPEALPALAVEEPTLKLTFGVSTSPFAGRDGQFSTSRQIGE
                     RLQRELETNVALRVEPTDQPDVFAVSGRGELHLSILIETMRREGYEFQVSRPEVITRN
                     VDGQTVEPVEHLVVDTIEEFVGAVTELVGGRKAKMIDMVNDGRGNVRLEFEIPTRGLI
                     GLRNEFLTRVKGNGSLSSRLIGFEPWQGPIQSNRSGALVAHETGTALSFGIAAAQERG
                     MTFIEPGQEVYEGMIVGQNARGTDLVVNVCKAKKLTNMRSSTGDIMVRLTPPVVLSLE
                     QALDFLADDELLEVTPKAFRLRKRLLQDHERARARKQA"
     gene            2164..4536
                     /locus_tag="VFV93_08160"
     CDS             2164..4536
                     /locus_tag="VFV93_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5165354.1"
                     /translation="MTGNQAVENLLGRVFKGYRVTRFIARGGMGLVFEGVQVSLDRSV
                     AMKFLYPHLSDDEQFRERFEREARAIANLDHPNIVRVLDFGTEDHMHFMILDYIDGVS
                     LRDHLIRVQADGLTLRTETVASITQQVGSALTFAHERGYVHRDVKPGNIMLAKNGRVF
                     LTDFGVVKDVGALQGTMTGAVIGTPEYMAPEQANGAPVGPSADLYALAVVTYEMLVGR
                     VPFQATTPVAVLTKHLHEPPPPPSTIAPWFGPELDAVFLRALSKNPNERFPTAAAFSE
                     ALIAALHAPRGSQTFPPGYVGVGTPTVVDSPMSHSAQTPMTPYGASYSQPVTGTPASG
                     TMHPMGMTPPPGGTAAGMPPSGVVPPSGMYPPAGATPVPSDSGRGRIPIIGAILIGLL
                     LILGAGAFYVLSQGDDDNGNLGATGDATATVKVAAQGTKTTVAEATKTPAGVETPTSA
                     VVETPTEPAGVATPTGEVAGTGGATPTVAEAAPTEVATPGADPIILFSSHRGDVHDSQ
                     IYAMEMDGTDQHQITFARGHSWGPRISPDGTMLFFSSVAPGEHQSHDAAGGGTTGTGN
                     HDIYVANLEGTTTADLTVTNINNITAGQLTWDNGWAWSPDGSLITFTSDRDGNWDIYR
                     VLPDGSDNQRLTDDPAQDGWPAWTPDGKHILFSSDRTGNWEIFIMDADGGNVRQLTDR
                     PDTTDLFPEVSPDGTRVVFSSQVQSTNEGEIYMMSIDGSNVTRLTNTAALNNMPSWCP
                     SGEQIVFTSDRDGNENIYMMTADGGTVTRLTDDPGEDTTPSCGYLPAPAP"
     gene            complement(4549..4998)
                     /locus_tag="VFV93_08165"
     CDS             complement(4549..4998)
                     /locus_tag="VFV93_08165"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5165355.1"
                     /translation="MRQRIRVSALVVRDGGLLLVRHVVDGREWWCPPGGGVEGDEPLT
                     VAAERELLEETGIRASSGRVVYLMDFINPEPACRNLEVYVWMDDASGEARVPDHERRY
                     LKEARFIARAEMAALTVYPVVLHDSFWDDLDAGVSETRYLGVKTVET"
     gene            complement(5012..5929)
                     /locus_tag="VFV93_08170"
     CDS             complement(5012..5929)
                     /locus_tag="VFV93_08170"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX5165356.1"
                     /translation="MAILKTVLAISVDGVIDRFTAITSGDVVDRLRDHAGAAVEAIVS
                     IAIVVIVAMLILRVLRSFVQNIVERVLSGNDQTQRDLQQKAQTLANVIESTGRFIVFV
                     IAGMMILSNLGFQIAPLLASAGIAGLAIGLGAQSLIKDTINGFFILMENQFGVGDVIA
                     VGASSGTVEEISLRRTVLRAVNGAQVIIPNGEIRTVENLSKGWSRAVLDIETAANVDD
                     AKVLDLLHEMLDHAQEDPLIGSKILEPPQILGISSISVTGVTFRVLVKTEPLQQWMVE
                     RELRLRIRQTFIENGIAMPVLTSATIAPS"
     gene            complement(6097..7419)
                     /locus_tag="VFV93_08175"
     CDS             complement(6097..7419)
                     /locus_tag="VFV93_08175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165357.1"
                     /translation="MSTIAIPRLRGMYYGWVMLLTLSFTEVTSWGILYYSFSVFLTPM
                     QDELGWSIAQMTGAYSAAILISGLAAIPVGRWLDRHGPRWLMTAGSIGATALVVAWSR
                     VDSLITFYVVWGLIGIALAAVLYEPALQAVAVWFRRKRSRALTILTFVGGFASVIYIP
                     LAAWLVNHYGWREALLILALILGVTTIPLHALVLRRRPADVGQAIDGDAVPADSPSTQ
                     RVAEPGITTREAMRTPVFWWLTAAFVLTYFGNIAITVHLIPYLVREGYSEGFAATAAG
                     MIGLLALPGRLVFTPLGGRIPRHYVTALIFSLQVAALLALLLIPGKTGVFTFVVLFGA
                     GFGAITPARASLVADLFGTANYGTISGVLAFYVTIARGIAPVAAGVLLGLAGSYTPVI
                     WTTVVVSAVATVAALQAKHGPAEVAPPTTHPLSSTAQRAPGSEEPPLR"
BASE COUNT         1374 a         2521 c         2375 g         1246 t
ORIGIN      
        1 gcctgcttgc gcgcccgggc ccgctcgtgg tcctggagga ggcgcttacg caggcggaag
       61 gctttggggg tgacttcgag gagttcgtcg tcggcgagga agtcgagcgc ctgttcgagc
      121 gagaggacga ccggcggcgt cagccggacc atgatgtcgc cggttgacga gcgcatgttg
      181 gtcagcttct tggccttaca gacgttgacg accaggtcgg tcccgcgcgc gttctggccg
      241 acgatcatcc cctcgtagac ttcctggccc ggctcgatga aggtcatccc gcgctcctgg
      301 gcggcggcga ttccgaacga gagggctgtc ccggtctcgt gggcgaccag cgcgccggag
      361 cggttggact ggatcggccc ctgccacggc tcgaaaccga tgagccgcga cgagagcgat
      421 ccgttgccct tcacgcgcgt caggaactcg ttgcgtagcc cgatcaaccc gcgcgtcggg
      481 atctcgaact ccagcctgac attgccgcgc ccgtcgttga ccatgtcgat catcttcgcc
      541 ttgcgcccgc cgaccagctc ggtgacggcg ccgacgaact cctcgatcgt gtcgacgacc
      601 aggtgctcga ccggctcgac cgtctggcca tcgacgttgc gggtaatgac ctccggccgc
      661 gagacctgga actcgtaccc ctcgcgccgc atcgtctcga tcaggatcga gagatgcagc
      721 tcgccacggc ccgagacggc gaagacatcc ggctggtcag tcggctcgac ccgcagcgcg
      781 acgttggtct ccagttcgcg ctgcaagcgc tcgccgatct ggcgcgaggt cgagaactgc
      841 ccgtcccgcc ccgcgaacgg cgacgtgctg acgccgaagg tcagcttcaa cgtcggctct
      901 tcgaccgcca gtgcaggcag cgcctcagga tggtccggat cggccagcgt cgcgctgacc
      961 ttcgcatcgg caaagccggt cagggcgacg atgtcgccgg cgaacgcttc ggacacctcg
     1021 cggcggctca gcccctcgtg cgtcgccagc cacgagacct tctgacgtgt ctggttgccc
     1081 tccaggtcga tgtggacgag cgtctcgccg gagcgaatca ccccgcgatt gatcctgccg
     1141 atcgcgatcc ggccgcggtg caggtcgtag tccagcgacg cgatctggag ctgggtcggc
     1201 ccagcgggat cggcgaccgg cgccgggatg actgacagga tcgtgtccag caacggccgc
     1261 aggtcatcgg ccagatcgtc cggtgcaaaa ccggcccggc cctcgcgggc gatcgcgtag
     1321 atgatcggga agtcgagctg gtcggcgtcg gtcgccaggt cgaggaagag gtcctgggtg
     1381 cgctcgatca cgttttccgg ctgggcgaac tgccggtcaa ccttgttgac gacgacgatt
     1441 ggccgcaggc cgattcccaa cgcgcgtgtc aggacagtcc gtgtctgtgg catcggccct
     1501 tcgacggcgt cgaccaccag cagacaacca tccgccatat tgaggacccg ctcgacctcg
     1561 ccaccgaaat cggcgtggcc cggtgtgtcg atgatgttga tcttgacatc gttataggtg
     1621 atcgacgcgt tcttcgacaa gatcgtgatc ccgcgctcgc gctcgaggtc gttggcatcc
     1681 atgatcagct cgccggccgc gtccgggtcg cggaagatgt gggactgctt cagcagcccg
     1741 tccaccagcg tcgtcttgcc gtgatcgaca tgcgcgataa tcgcgatgtt ccgcagcgcg
     1801 ggtcgagtca tcgtctgtcc tgttcgttcg tttactgctg tcgcgaccac gtatccctcg
     1861 accctgttgt atccaacgca aagctgaatc gcacaaaatg gcccgcgcca tgctcagcgc
     1921 gggccgtcac cgaacggata atgataccac gatgaccatg attcaacgtg ccatcggtag
     1981 gggccgggac ggagccacgg tccctaccga tgggcgggcg agacgatgat gttcgatcag
     2041 gacgatcatg gcattcatcc gggcaataca aagtgctaat atgatgcgta tcccacaggt
     2101 gaccgaccag cgaggccgct ggtagtctgg catcgacgct acacaatgta tcggagaatc
     2161 ccgatgaccg gcaatcaagc ggttgaaaat ctgctcggga gagtgttcaa gggctatcgg
     2221 gtgacccgct tcatcgcgcg gggcgggatg gggctggtct tcgaaggagt ccaggtctcg
     2281 ctcgaccgat cggtcgcgat gaagttcctc tacccgcacc tgagcgacga cgagcagttc
     2341 cgcgaacgct tcgaacgtga ggcgcgcgcc atcgcaaacc tcgatcaccc caacatcgtt
     2401 cgcgtgctcg atttcggcac cgaggatcac atgcacttca tgatcctcga ctacatcgat
     2461 ggcgtgtccc tgcgcgatca cctgatccgc gtccaggccg acggcctgac gctgcggacc
     2521 gagacggtcg ccagcatcac ccagcaggtc ggcagcgcgc tgacattcgc ccacgagcgc
     2581 ggctacgtcc accgcgacgt caagcccggc aacatcatgc tggccaagaa tggccgcgtc
     2641 ttcctgaccg actttggtgt cgtcaaggac gtcggggcgc tacagggaac gatgaccggc
     2701 gccgtgatcg gcacgccgga atacatggcg cccgagcagg ccaatggcgc gccagttggg
     2761 ccgtcagccg acctgtatgc gctggcagtc gtcacctacg agatgctggt cggaagggtg
     2821 ccgttccagg cgacgacacc ggtcgccgtc ctcaccaagc atctgcacga gccgccgccg
     2881 ccgccgagca cgatcgcgcc ctggttcggt ccggagctgg acgccgtctt cctgcgcgca
     2941 ctttcaaaga atcccaacga gcgcttccca acggctgcgg cgttcagcga ggctttgatc
     3001 gctgccctgc acgcgccgcg cgggtcacag acgttcccgc ccggctatgt cggcgtcggg
     3061 acaccgaccg tcgtcgactc gccgatgtct cattcagcgc agacgccgat gacaccctac
     3121 ggcgccagct actcacagcc cgtgaccggt acgccggcgt ccggcacgat gcatccgatg
     3181 gggatgacac ctccgccagg tggaaccgcc gccgggatgc cgccctccgg tgtcgtgccg
     3241 ccatccggca tgtacccgcc ggccggtgcc acaccggtgc cgtcggattc cggtcgtggg
     3301 cggattccga tcatcggtgc gattctgatc gggttgctgc tcatcctcgg cgccggggcg
     3361 ttctatgtgc tgtcgcaggg cgatgacgac aatggcaacc tcggcgcgac cggcgacgcc
     3421 accgcgactg tgaaagtcgc cgcccaagga accaagacaa ccgttgcgga agctaccaag
     3481 acgccggctg gagtggagac accgaccagc gccgttgtag aaacgccgac cgagcctgct
     3541 ggcgtcgcga caccgacagg cgaggtggcc ggcactggcg gtgcaacgcc aaccgttgcc
     3601 gaagccgccc cgaccgaggt cgccacgccg ggggccgatc cgatcatcct cttctcatcc
     3661 catcgcgggg acgtgcacga ctcgcagatc tacgcgatgg agatggacgg gacagaccag
     3721 caccagatca ccttcgcacg cggtcactcc tggggtccgc ggatctcgcc ggatggcacg
     3781 atgttgttct tctcgtcggt cgcgccaggc gagcaccagt cgcacgatgc ggccggcggt
     3841 gggacgaccg gcaccggtaa ccacgatatc tacgtcgcca acctcgaagg cacgacgacg
     3901 gccgatctga ccgtgacgaa catcaacaac atcacggccg gccagctgac ctgggacaac
     3961 ggctgggcct ggtcgccgga cggctcgctg atcaccttta cgtccgatcg ggacggcaac
     4021 tgggacatct accgggttct gccggacggc agcgacaacc aacgcctgac cgacgatccg
     4081 gcccaggatg gctggccggc ctggacgccg gacgggaagc acatcctctt ctcgtccgac
     4141 cgaaccggca actgggagat cttcatcatg gatgcggacg gcgggaacgt ccgccagctg
     4201 accgaccggc ccgacacaac tgatctgttc ccggaagtct cgccggacgg gacgcgcgtc
     4261 gtcttctcat ctcaggtcca gtccaccaac gagggcgaga tctacatgat gtcgattgac
     4321 ggcagcaacg tcacccggct gacgaacacg gccgcgctca acaacatgcc gtcgtggtgt
     4381 ccgtccggcg aacagatcgt cttcacctcc gaccgtgacg gcaacgagaa tatctacatg
     4441 atgacggcgg acggcggcac cgtcacccgg ctgaccgacg atcccggcga ggacacgacg
     4501 ccgtcctgcg gctacctccc ggctcccgcg ccgtagccgg cgagtggctc aggtctcgac
     4561 ggtcttgacg cccaggtagc gcgtctcgct gacgcctgcg tcaagatcgt cccagaagct
     4621 gtcatggaga acgactggat agaccgtcag tgctgccatc tcggcgcggg cgatgaagcg
     4681 cgcctctttc aggtaacgtc gctcgtgatc cggcacgcgc gcttcgccac tggcgtcgtc
     4741 catccagacg tagacttcga gattgcggca ggcaggctcg gggttgatga agtccatcag
     4801 gtagacgacg cgcccgctgc tggcgcgaat cccggtttct tcgagcagct cccgctcggc
     4861 ggcaactgtc agcggctcgt cgccctcgac cccgccgcct ggcgggcacc accactcgcg
     4921 cccatcgacg acatgccgga ccagcagcag cccgccgtcg cgaacgacga gggcagacac
     4981 ccggatgcgc tggcgcatgg tccctcccga atcaggacgg cgcgatcgtt gccgatgtca
     5041 ggaccggcat tgcgatcccg ttttcgatga acgtctggcg aatgcgcagg cgcagctccc
     5101 gctcgaccat ccactgctgc aacggttcgg tcttgaccag aacccggaat gtgacaccgg
     5161 tgacactgat cgagctgatc cccaggatct gtggcggctc gaggatcttg ctgccgatca
     5221 acgggtcttc ctgtgcatga tcgagcatct cgtgcaacag gtccagcacc tttgcatcat
     5281 cgacgttggc ggccgtctcg atgtcgagaa ccgcccgtga ccagcctttc gacagattct
     5341 cgaccgtgcg gatctcgccg ttcgggatga tcacctgcgc gccattgacg gctcgcagga
     5401 ccgtccgccg cagggatatc tcctcgaccg ttccggacga cgccccgacg gcgatcacat
     5461 cgccgacgcc aaactggttc tccatcagga tgaagaagcc gttgatcgtg tccttgatca
     5521 ggctctgggc gccgaggccg atcgcgagac cagcgatacc ggcactggcc agcagcggcg
     5581 cgatctgaaa gcccaggttc gagaggatca tcatccccgc gatgacgaag acgatgaacc
     5641 gtccggttga ttcgatgacg ttggcgagtg tctgggcctt ctgttgcagg tcgcgctgtg
     5701 tctgatcgtt acctgacagc acccgctcga cgatgttctg aacgaacgag cgcagcaccc
     5761 gcaggatcag catcgcgacg atgacgacga ttgcgatcga aacgatcgcc tcgacggcgg
     5821 ctccggcatg atcgcgcagc cggtcgacga cgtcgccgct ggtgatcgcc gtgaaccgat
     5881 caatcacccc atcgaccgag attgccagca ccgtcttcaa tatcgccacc aacgtccggt
     5941 gtgcacgatc gagcccgcca caccaccatc gaatccgacc cgatacctta gcgtagcaaa
     6001 cgcattcggt cgaatgtcga aggatgttcc ctgtcgtcca ccgcccagag ggcacccggc
     6061 agcgaaagac ctcccctgcg ctgaagccat gtgtggttag cgtaggggag gctcttcgct
     6121 gccgggtgcc ctctgggcgg tggacgacaa ggggtgcgtt gtcggtggtg cgacctcggc
     6181 cgggccgtgc ttcgcttgca gagccgcgac tgttgcgacg gccgagacga cgaccgtcgt
     6241 ccagatcacc ggggtgtagg agccggccag ccccagcaac actccggctg cgaccggcgc
     6301 gatgccgcgc gcgatcgtga cgtagaaggc caggacgccg ctgatggtgc cgtagttagc
     6361 ggtgccgaac aggtcggcga ccagcgatgc ccgcgccggt gtgatcgcac cgaaaccggc
     6421 cccgaacagg acgacgaacg tgaagacacc tgtcttgccg ggaatcagca gcagcgccag
     6481 cagcgccgcg acctgcaacg agaagatcag cgccgtcacg taatggcgcg gaatccggcc
     6541 accgagcggc gtgaagacca gccgccctgg caacgccagc aggccgatca tcccggctgc
     6601 cgtcgccgcg aacccctcgc tgtacccttc gcggaccaga tacgggatca ggtgaacggt
     6661 gatggcgatg ttgccgaagt aggtcaggac gaatgcggcg gtcaaccacc agaagacggg
     6721 cgtccgcatc gcttccctgg ttgtgatgcc tggttctgca acccgctgcg tgctgggact
     6781 gtcggcgggg accgcgtctc cgtcaatcgc ttgaccgaca tcggccgggc gccggcgcag
     6841 gacgagcgcg tgcagcggga tcgttgtgac gccaaggatc agcgccagga tcagtagcgc
     6901 ctcacgccaa ccgtagtggt tcaccagcca ggccgccaac ggaatgtaga tcacgctcgc
     6961 gaagccgccg acgaacgtca ggatcgtcag cgcccggctg cgcttgcgcc ggaaccagac
     7021 cgcgacggct tgcaacgccg gctcgtacag cacggccgcc agtgcgatcc cgatcaagcc
     7081 ccacacgaca taaaacgtga tcagcgaatc gacccgcgac cacgccacca cgagcgccgt
     7141 cgccccgatc gagccggccg tcatcagcca gcgcgggccg tgccggtcga gccagcgacc
     7201 aaccgggatg gcggccagcc cggagatcag gatcgccgcc gagtaagcgc cggtcatctg
     7261 cgcgatcgac cagcccagct cgtcttgcat cggtgtcagg aagacggaga aggagtaata
     7321 gaggatgccc caggaggtga cctcggtgaa cgacaaggtc agcagcatga cccagccgta
     7381 atacatcccg cgcagcctcg ggatcgcgat cgtgctcacg ccgtcacccc ctccccacga
     7441 ttttaacggg gggagcgacc ttctcgcatc aggggatgcg cacaaccgga ttggtgattc
     7501 cgcacaaggc ctgatc
//
LOCUS       DASUGJ010000368         5879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1169022, whole genome shotgun sequence.
ACCESSION   DASUGJ010000368 DASUGJ010000000
VERSION     DASUGJ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169022"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /gene="manB"
                     /locus_tag="VFV93_08180"
     CDS             complement(<1..392)
                     /gene="manB"
                     /locus_tag="VFV93_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922131.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX5165358.1"
                     /translation="MSASQIGSLFKAYDIRGVAPEELTPEIAYRIGRALVVYLGADNV
                     CVGRDMRVSGPLLASALIEGIRDQGADVTDIGLISTDGLFFAVGKFGFPAGVMVTASH
                     NPAEYNGFKLCREQARPLSMDQGIGEIR"
     gene            complement(677..1135)
                     /locus_tag="VFV93_08185"
     CDS             complement(677..1135)
                     /locus_tag="VFV93_08185"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5165359.1"
                     /translation="MPAVTLQPITTRRVARNVVTLRLGPGQERFVAPNAISMADAYVE
                     KSWHPLAVFAGDELVGFVMYGTDVVQDPEQWWIIRVMIDERFQGCGYGRAATTALVDL
                     MRERHGCVSIRLGVDPANQRAIRLYESLGFRDTGEVEDDELIMQCGMGDA"
     gene            1263..1727
                     /locus_tag="VFV93_08190"
     CDS             1263..1727
                     /locus_tag="VFV93_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165360.1"
                     /translation="MNQNHTSIPRMQLDRILTSAGDRKLAMVTIATVLMIGMSDTIAA
                     ALGYVVGSDGQLGGRVLNFAIDAGAFSFLLLYSLLRSRNEPTQLSDHLSPLSRQVMIV
                     VSTAAAYGLFFVVDVLDVSYPAKLLLIAIPVGALFLVAMTARMSAKREPKEH"
     gene            complement(2248..3012)
                     /locus_tag="VFV93_08195"
     CDS             complement(2248..3012)
                     /locus_tag="VFV93_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX5165361.1"
                     /translation="MAHRDEIIGYCDERLRSAAFQDVAVNGLQVSGRLQINRLALAVS
                     TSRTTLTEAANWRADALLVHHGLLWGGKVGPITGVFADRLRLIFENELNLIAYHLPLD
                     GHPEIGNCARLCIASGYMPAGRFANVGAEPLGIVGEWPQGHSLDDLVGTLADLTEREP
                     VVVGIDDGQRQIERAGFLTGSGYGALQDAVGAGCQALVTGDVRESTMAEARELGLSVI
                     AAGHEATERLGVQALAAELAAKFNLTTHFIDDPNPI"
     gene            3106..4053
                     /locus_tag="VFV93_08200"
     CDS             3106..4053
                     /locus_tag="VFV93_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5165362.1"
                     /translation="MREAGTLAPDDAVDLHMHTFASDGRWSPDELTAYLVANDFRLVA
                     LADHDTMYSVPEMSRRTREAGIAYIPAVEMTTRWEQRQVHLLVYGIDPEASSSAPFMA
                     VLETQQRQLRETSERMIELLDKHCRYIPSLHELPEIKLGLQLTPHRVFTAMIQNGHGN
                     NLYQAHNIVRGLGEPVVVDVPLDETVAAAHASGALTIVAHPGRDDGSGVLKHEDLDRM
                     LAEIPIDGVEAHYRSYKDVDTSYYRDWAERNNLLVSSGSDSHWPNHPVNPIAHKARWV
                     APLLERLGYNVAPWEGPAWMPAPPLAPSDGDPATSSPTD"
     gene            4058..4543
                     /locus_tag="VFV93_08205"
     CDS             4058..4543
                     /locus_tag="VFV93_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5165363.1"
                     /translation="MTLLRLAQDDMLFHVRIAAVCLHDGHVLLQGSVHEGFWILPGGH
                     CELLEPTSETVRREMREEYDVEVDVGRLLWVVENFFTYERTRTHQIAFLYEVHLPDGS
                     DLLDVANDITGIDGDVPFVARWFPIDSLSDVWLLPSFLREALKSLPETPQHIVHLDAE
                     H"
     gene            4644..>5879
                     /locus_tag="VFV93_08210"
     CDS             4644..>5879
                     /locus_tag="VFV93_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5165364.1"
                     /translation="MFYRLGRFAYLHRRLVIAAWLLCCIISAPLILRLPSVLEVGGFS
                     NDQIEAARARTVLEAEIPSFSSSVMVVIFQSDELRATDPAFVDEARTALTGVLAMPEV
                     TSVVSFVDNPRQISTDGRTAYTLIRLAPSPEESQRLMPEVRDRLAATDLDVTLAGAPA
                     FYEDVERISEQDLRRAEAIAIPFALLALLLVFGSVVGAVVPLVVGGLSVAAVLGLIFV
                     VAGVVDMSIFVLNLTTMLGLGLAIDYSLFMTSRFREELQRRSVADAVAVTTGTAGRAV
                     FFSGLTVLIGLSGLVIFNFMFLRSVGIAGALVVLVAVIGALTLLPAVLGVVGTRIDRL
                     RLVPARPERADSGFWHGLAHWVMAHPWFVFVPTLVLLLALGLPFLNVKVSSPDATILP
                     LRVESRQGFETLRREFGSGE"
BASE COUNT          954 a         1983 c         1782 g         1160 t
ORIGIN      
        1 tcgcgaatct cgccgatccc ctggtccatc gacaacggcc gtgcctgctc gcgacagagc
       61 ttgaagccgt tgtactcggc cgggttgtgc gaggccgtca ccatcacgcc ggccgggaag
      121 ccgaacttgc cgaccgcgaa gaagagccca tcggtcgaga tcagcccgat gtcggtcacg
      181 tccgcgccct gatcgcgaat cccctcgatc aatgccgacg ccagcagcgg ccccgagacg
      241 cgcatatccc gcccgacaca gacgttgtcc gcccccagat aaacgaccaa cgcccgcccg
      301 atccggtagg cgatctccgg cgtcaactcc tccggcgcaa ccccgcgaat gtcatacgcc
      361 ttgaacaggc tcccaatctg gctagcgctc atcaggttcc tcttttcgtg acgcgtcacg
      421 cgtgacgcgt gatgcgtcgg catgcgtatt gtcaccaggg tgcacggcat gggtgaggac
      481 cgtacccgtt gccctgtcat cctgagccgc agcgaaggat cgctcggcgt tcaggttacg
      541 aacggtcgcc caggatgcga acgcgcagct accgaatcac ctgctcgtgc ccctaacgcc
      601 aggcgatcct tcgctgcggc tcaggatgac agggcaacgg gtacggtcct cacccatgcc
      661 gttcgtcctc accgcgtcac gcgtcaccca tcccacactg catgatcaac tcgtcgtctt
      721 caacttcacc cgtgtccctg aaacccaacg attcgtacag tcgtatcgct cgctgattcg
      781 caggatcgac gccgagcctg atgctcacac acccgtgacg ctcgcgcatc aggtcaacca
      841 gcgccgtcgt cgccgcccgg ccatagccgc acccctggaa gcgctcgtcg atcatcaccc
      901 gaatgatcca ccattgttcc gggtcttgaa ccacatcggt tccatacatc acgaacccga
      961 ccaactcatc cccggcaaag accgccagcg gatgccaact cttctcgacg tacgcatccg
     1021 ccatagagat cgcgttcggc gcgacgaacc gctcctgccc cggcccgagc ctgagcgtca
     1081 cgacattccg cgccacccgc ctggtcgtga tcggctgtag cgtgaccgcc ggcattgccg
     1141 ccctttcaca aaactgacga aagtaccacg agagaccaat tgtgcgttcg cgtcactggg
     1201 tgtagggttc aatctcacga tcaaccaact gcggatcgca tcgcacaacg gacgagggcc
     1261 gcatgaatca gaaccataca tccattccaa ggatgcaact cgaccgcatc ctgacatcgg
     1321 ccggcgatcg caaacttgcg atggtcacga ttgcgactgt cctaatgata gggatgtccg
     1381 acacgattgc agcggcgctc ggctacgttg tcggtagtga cggtcaactt ggcgggcgag
     1441 tcctcaattt cgcgatcgat gccggtgcgt tctcgtttct cctactctac tccctcttga
     1501 ggtcacggaa cgagccaact caactcagcg accatttgag tcctctcagt cgccaagtga
     1561 tgatcgtcgt gtcgactgcg gcagcgtacg ggttgttctt tgttgtagat gttctcgacg
     1621 tgtcgtatcc ggccaagctg ctgctcattg ccatccctgt tggcgcgctg ttcttggttg
     1681 cgatgactgc ccggatgtct gccaagcgcg agccaaagga gcattagacg acaagtcaat
     1741 cgtccaaggg ggtcgagatc agcagcattc gttcactgat acgtccaaat cgcagcgacg
     1801 tcattcgctg gtatgttcag atcgcgatgc ttgggttgtt cctggtttcg cgggacattt
     1861 cagagtacgt cacggacttg gcgggtattc agaacggaac gtggcgctgg ctcatcagtt
     1921 tgggagcggg cgttttgccc ctgtttgtat tcgcccggtt cgtcaagcag ccagtcgcga
     1981 cgaggcaagg caccgatgaa ggatcgtgac tcgaacatct tctggcggta tcgttcattt
     2041 atcgttcttc ccgtcctgat gtcattttgg acgatcgtta ttacccagtt tgtcgatgcg
     2101 tctcttgttg tgacattcga actctggatc gtcgttccct tggtcgtttt ggtcacatac
     2161 atcgtcgtta gttcacgacg aatgtgatct cagcaatcgc ccaccgagtt cgagccttgc
     2221 gagcgcccac atatccatcg cgagtgatca aatcgggttc gggtcgtcga taaagtgggt
     2281 cgtgaggttg aacttggcgg ccagttcggc tgccagggcc tggacgccga ggcgttcggt
     2341 ggcctcgtgg ccggcggcga tgacggagag gcccagttcg cgggcttcgg ccatcgtgga
     2401 ttcgcggaca tcgccggtga cgagcgcctg gcagccggcc ccgacggcgt cctggagggc
     2461 tccgtagccg gaaccggtca ggaagccggc ccgttcgatc tggcgctggc catcgtcgat
     2521 gccgacgacg accggttccc gttcggtcag gtcggccagc gtgccgacca ggtcgtcgag
     2581 gctgtgtccc tgcggccact cgccgacgat gccgagtggc tcggccccga cgttcgcgaa
     2641 ccggccggcc ggcatgtagc cgctggcgat gcagagccgg gcgcagttgc cgatctccgg
     2701 gtggccgtcc agcggcaggt ggtaggcgat caggttcagc tcgttctcga agatgagccg
     2761 cagccggtcg gcgaagacgc ccgtgatcgg cccgaccttg ccgccccaga ggagtccgtg
     2821 gtggaccagc agtgcgtcgg cccgccagtt ggccgcctcg gtcagcgtcg tccgcgaggt
     2881 gctgacggcc agcgccagcc ggttgatctg gagccggccg ctgacctgga ggccgttgac
     2941 cgccacatcc tgaaaggccg cgctgcgtag ccgctcgtcg caatagccga tgatctcgtc
     3001 gcggtgcgcc atgaccggct cctctcgttc cgttcgcccc cgattgtgcc atccgtcggt
     3061 atagttgcgg tgatcggtgt cgggtgtcag gtgacaggag ctattgtgag agaggctgga
     3121 acgctggcgc cggatgacgc cgttgacctg catatgcaca cgttcgcgtc ggacggccgc
     3181 tggtcgccgg atgagttgac cgcctacctg gtcgccaacg acttccggct ggtcgcgctg
     3241 gccgaccacg acacgatgta ctcagtcccg gagatgtccc gtcgcacccg cgaggccggc
     3301 attgcctata tccccgccgt tgagatgacg actcgctggg agcagcgcca ggtccacctg
     3361 ctcgtctacg ggatcgaccc cgaggcgagt tcgtcggctc ccttcatggc cgtgctggag
     3421 acgcagcagc gtcagcttcg cgagacctcc gagcggatga tcgagctgct cgacaagcac
     3481 tgccgctaca tcccgtcgct gcacgagctg ccggagatca agctcggcct gcagctcacg
     3541 ccgcatcgcg tcttcacggc catgatccag aatggccacg gcaacaacct ctaccaggcg
     3601 cacaacatcg tccgcggcct cggcgagccc gtcgtcgtcg atgtcccgct cgatgagacg
     3661 gtcgcggcgg cccacgcctc cggcgcgttg acgatcgtgg cccaccccgg ccgcgacgac
     3721 ggctccggcg ttctcaagca cgaagacctc gaccggatgc tggccgagat cccgatcgac
     3781 ggcgtcgagg cccactaccg ctcctacaag gacgtggaca ccagctacta tcgcgactgg
     3841 gccgagcgca acaacctgct cgtctcctcc ggctccgact cccactggcc caaccatccg
     3901 gtcaacccga tcgcccacaa ggcccgctgg gtcgccccac tcctcgaacg cctgggctac
     3961 aacgtcgccc cctgggaggg accagcctgg atgccggccc cgccactcgc gccatctgac
     4021 ggcgatccgg caacgtccag tccgacggac tgaggccatg acgttgctcc gcctcgctca
     4081 ggacgacatg ctgttccacg tccggattgc cgccgtctgc ctgcacgacg ggcacgtcct
     4141 gctgcaaggc tcggtgcacg aaggcttctg gatcctgccg ggtggtcact gcgaactgct
     4201 cgaaccgacc agtgagactg tccggcgtga gatgcgcgag gaatacgatg tcgaagtcga
     4261 cgtcggccgg ctgctgtggg tcgtcgagaa tttctttacc tacgagcgca cacgaacgca
     4321 tcagatcgcg ttcctctacg aggtgcatct cccggacggc agcgatctcc tcgacgtggc
     4381 gaacgacatc accggcatcg acggcgacgt ccctttcgtc gcgcgctggt tcccgatcga
     4441 ctcgctgtct gatgtctggt tgctgccctc cttccttcgc gaggcgctca aatcactccc
     4501 agaaacgccg caacacatcg tccatctcga tgcagaacat tgatctgccg gccggccgtc
     4561 ccgcgcggac ggcgggggtg cggttcctgc atagttgagg cagcgcgcag gagcgccacg
     4621 ggatcacgct cagacaggtt ggcatgttct acaggctcgg tcgtttcgca tatctccacc
     4681 gccggttggt gatcgccgcc tggctgctct gctgcatcat ctccgcgcca ttgatcctgc
     4741 ggctgccgtc ggtgctggaa gtcggcggct tctccaatga tcagatcgag gcggctcgcg
     4801 ctcggacggt cctcgaagcg gagattccgt cgttctcgtc ttcggtcatg gtcgtcatct
     4861 tccagtccga cgagctgagg gcgaccgacc cggcgttcgt cgacgaagca cggactgcgc
     4921 tgaccggtgt cctcgccatg ccggaagtca ccagcgtcgt cagcttcgtc gacaacccgc
     4981 gccagatctc gaccgatggc cggacggcct acacgctgat ccggctcgcg ccgtcccccg
     5041 aagagtcgca acggctgatg ccggaggtcc gtgaccggct ggccgcgacc gacctcgatg
     5101 tcacgctggc cggcgcgcct gccttctacg aggatgtcga gcgcatctcc gagcaggatc
     5161 tgcgccgggc cgaggcgatc gcgattccgt tcgcgttgct ggcgctgctg ctcgtcttcg
     5221 gctcggtcgt tggcgcggtc gtgccgctcg tcgtcggcgg gctgagcgtt gcggccgttc
     5281 tcgggctgat cttcgtcgtc gccggtgtcg tcgatatgtc gatcttcgtc ctcaacctga
     5341 cgacgatgct cggcctcggt ctggcgatcg actactcgct cttcatgacc agccgctttc
     5401 gcgaggagct tcaacgccgc tccgtcgcgg acgccgtcgc cgtcacgacc gggacggccg
     5461 gccgggccgt cttcttctcc ggcctgacgg tgctgatcgg cctctccggg ctggtcatct
     5521 tcaatttcat gttcctgcgc tcggtcggca tcgccggggc gctggttgtt ctcgtcgccg
     5581 tcatcggcgc gctgacactg ctgccggccg tcctcggcgt cgtcgggacg cggatcgacc
     5641 ggctccggct ggtcccggcc aggcctgagc gggccgacag cggcttctgg cacgggctgg
     5701 cccattgggt gatggcccac ccgtggttcg tcttcgtgcc aacgctcgtc ctgctgctgg
     5761 cgctcggcct gccattcctc aacgtcaagg tctcctcgcc cgacgcgacc atcctgccgc
     5821 tgcgggtcga gtcgcggcag ggattcgaga cgctgcggcg cgagttcggc agcggtgag
//
LOCUS       DASUGJ010000369         4487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1030900, whole genome shotgun sequence.
ACCESSION   DASUGJ010000369 DASUGJ010000000
VERSION     DASUGJ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030900"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFV93_08215"
     CDS             complement(<1..291)
                     /locus_tag="VFV93_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165365.1"
                     /translation="MTTMATMDWAMQALGISIVPAGRSEAPACAIGLDGRSIDAEVIV
                     IEAAPDVQRPIAVAGIQPADVRLVMLERDAPGNVFPPVEHTEGRSDVIHVAHG"
     gene            complement(522..848)
                     /locus_tag="VFV93_08220"
     CDS             complement(522..848)
                     /locus_tag="VFV93_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX5165366.1"
                     /translation="MTAATPVSTEHIKIVEGVTGPKAQIVGHRIRVKDIVIWHEHRQM
                     SVDEIIELFPQLTRSDIHAALACYWDNKDDIDALMAANDAMVDRDRQVAPPSRFAEKL
                     KQLESQ"
     gene            complement(1078..>4487)
                     /locus_tag="VFV93_08225"
     CDS             complement(1078..>4487)
                     /locus_tag="VFV93_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165367.1"
                     /translation="LLWSASDDVGLRNFLPVALVGILLLVVVAGGPLWLAKMLGVRGT
                     ALVGFGFVFGCSIAIVVTIGSVNAVLGYALAPAALTLSAMFAAGRLDSRPAAAVVAVT
                     AVLYVVMVITDADSDIRNFLFAGLVAWVIVPAIGALRQSEEAQGEATPAHPTTPSVAP
                     STTVSIVVQHLQSWRTAHRRTFRAGIVALAIVAGTVSCAGVLSRIQNAELSASIHVTG
                     PLYGYVEGNGSVAGFPSPGGGIVGTTGYEDDVLVFAPDARFPSATHTFHYDHGGYTAH
                     LRVTDPGPAFIAVNSVNESGGGLFGGDDEVKGRAIFVVSAPVNAQLRLKGDTARSVGK
                     LRLEVDRDGDGTFDETLKPDFVKTGDDIDDYGQFKTTATVDSNGTTDARVSLTTQGKD
                     RVDRTYIWSFPANPLPQLYDAPIELTDPSYLLFASIDVDGNLERVQEARILGGAPVIE
                     DSIDLDTERTISFREPGQVARLTFEGHRDQRIVARVTASDLGALPCCTGRMQVLGPDD
                     TALASETGIGANPSAMETVVLDRDGVYTLIVNPYLAYTGDVTVVLETLDDATARLRPN
                     GQPMTLSFEHVAQQGLVTFYGAAGQQVSVVIKIVEGFCCSADAELLAPDGTPIANGRL
                     TLGDDVLTMRALLAVDGVYTVALAPNLQKPGELTVELNSIEVVETTVSLDAPATEVEI
                     GTPGQSAQLRFDGSAGQQLYVTAHDSIATDNGLGTAFGDFRTRLSVRSEDSTDEIVRA
                     WLVPRDDSVVAVTLPGSGRYVIEIDPPTDATGTATIGLSSTLPERATLTAAALPVNHP
                     AETLSIDDPKSLVQRSFTAVEGQLVTVAIQDVVGEELDHCCSYWPHLFDPHGNEISYD
                     IETRLNLFGTDGVNEALRFPETGVYVLEVQSYETATGQFSLAFSSSDDLKDEIAIGDS
                     PATIMTSQPGQTARVTFTGQAGDVFVNVAMAEQGRTTDDGVTYTIPDAERWFDHESVT
                     ITPGLRSHTRFTLPQDGMYTIVFQPTAQTTFAISLSITLTEDESTPVPEIVPQPITVD
                     GDPIDVSLADGSTGLTRAALDFSGKQGERLTIDIEVAENLHEDACCGYHVVVVGPSTP
                     ALVAERDLTKGRTRLEVTLPSTGDYRIWLRTVGNSSGTLRVSVTSID"
BASE COUNT          811 a         1416 c         1422 g          838 t
ORIGIN      
        1 tccatgggcg acgtgaatga catccgagcg tccctccgtg tgctcgaccg gagggaagac
       61 gttgcccgga gcatcacgct ccagcatcac gagacggacg tctgccggct gaatgccggc
      121 taccgcgatt gggcgttgaa catccggcgc ggcctcgatg acgatgacct ccgcatcgat
      181 gctgcggcca tccagtccga tcgcacaggc tggcgcctcg ctgcgccccg ccgggacgat
      241 gctgatcccc agcgcctgca tcgcccagtc catcgtcgcc atcgtcgtca tcgcctgcac
      301 catgcccctc gtgacgcggt aacctcgaac cactgtcacc aagcataggc ggcaagtatc
      361 gcgacgattt cacggaaatg tcacaacttt gtgaactgat cgggacgtgg tcaactggac
      421 ataaaatgaa tccgtgggcg actcttgggt gattgatcgg ctgagtctgg agagatcctt
      481 cggctcacgc ctcaggatga caaagggcgt acgacacagc ttcactgcga ttcgagctgc
      541 ttcagctttt cggcgaagcg gctcggcggt gctacctgac gatctctatc gaccatcgca
      601 tcgttggcag ccatcaacgc atcgatgtcg tccttgttgt cccagtaaca tgccagcgcg
      661 gcgtggatgt ccgaccgcgt cagttgaggg aacaactcga tgatctcgtc aacggacatc
      721 tgacggtgct catgccaaat gacaatatcc ttgacgcgga tgcgatgacc aacgatctgg
      781 gctttcggcc cggtcacgcc ttcaacgatc ttgatgtgtt cagtactcac tggtgtcgct
      841 gctgtcatca tctgtctcca tctcaggaac aggtcggcgc cgtgattgca gcaacgccaa
      901 tacagatccc cgccggcaac gctgacacac tcctttcttc caacggactt tgcgtacaag
      961 agcgattatc cgcaaaacac acagaacaga gttcggagcc gatgctcgcc tctcaccggc
     1021 tggcggggat cgagtggcca ccttccacga cgaactcaat gaggctgatc ggcgcggtta
     1081 gtcgatggac gtgacggaga cgcgcagggt gccgctgctg ttgccgaccg tccggagcca
     1141 gatccggtag tcgccggtgc tgggcagcgt gacctcaagc cgcgtcctgc ccttggtcag
     1201 gtcacgttcc gcgacgaggg ccggcgtcga cggcccgacc acgacgacgt ggtacccgca
     1261 acacgcgtcc tcatgcagat tctcggcgac ctcgatgtcg atggtgagcc gttcgccctg
     1321 cttgccggaa aagtcgagcg ctgctcgcgt caggccggtg ctcccatcgg ccagcgagac
     1381 gtcgatcggg tcgccgtcga ccgtgatcgg ttgcgggacg atctccggaa ccggcgtcga
     1441 ctcgtcttcg gtcagtgtta tggaaagcga gatagcgaag gtcgtttgtg cggtcggctg
     1501 gaacacgatt gtgtacatgc cgtcctgcgg cagcgtgaag cgtgtatggg atctcagccc
     1561 cggcgtgatc gtgacacttt cgtggtcgaa ccagcgctcg gcgtcaggga tcgtgtaggt
     1621 cacgccgtcg tcggtggtgc ggccctgctc ggccatcgcg acgttgacga agacatcgcc
     1681 ggcctggccg gtgaacgtca cgcgcgctgt ctggcccggc tggctggtca taatggtggc
     1741 gggtgagtca ccgatcgcga tctcgtcctt caggtcgtcg ctggagctga acgccagact
     1801 gaactggccg gtggcggtct cgtaggactg gacttccagc acgtagacgc cggtctccgg
     1861 gaagcgtagc gcctcgttga cgccgtcggt gccgaacaga ttcagccgcg tctcgatatc
     1921 gtatgagatc tcgttcccgt gcgggtcgaa gaggtgcggc cagtagctgc aacagtggtc
     1981 cagctcctcg ccaacgacat cctggatcgc taccgttaca agctgtccct cgacggcggt
     2041 gaacgagcgc tgcacgagcg acttcggatc gtcgattgag agcgtctcgg ctggatggtt
     2101 cacgggcagc gcggcggccg ttagcgtcgc acgctccggc agcgtggaag acagaccgat
     2161 cgtcgcggtg ccggttgcgt cggtgggtgg atcgatctcg atgacgtagc gcccgctgcc
     2221 gggcaacgtg acggcaacga ccgagtcgtc tcgcggcacg agccacgcgc gaacgatctc
     2281 atcggtgctg tcctcgcttc gcacactcaa ccgggtgcgg aagtcgccga aggctgttcc
     2341 gaggccgttg tcggtcgcga tgctgtcgtg ggcggtgacg tagagctgct gacccgccga
     2401 gccgtcgaac ctcagttgcg cactctggcc cggagtgccg atctcgacct ctgtcgccgg
     2461 cgcgtcaagg ctgacggttg tctcgacaac ttcgatgctg ttgagttcga ccgtcagctc
     2521 gccgggtttc tggaggttcg gcgcaagtgc caccgtataa acaccatcga ccgccagcaa
     2581 ggcgcgcatc gtcaggacat cgtcgcccaa cgtcaacctg ccgttggcga tcggcgtgcc
     2641 gtccggcgcc agcagttcgg catcggcgct gcaacagaag ccctcgacga tcttgatgac
     2701 gacgctgacc tgctgcccgg ccgcgccata gaacgtcacg agcccctgtt gcgcgacgtg
     2761 ctcaaacgat agtgtcatcg gctggccgtt tgggcgcaat cgtgccgtcg cgtcgtccag
     2821 cgtttcgagg acgaccgtga cgtcgccggt gtaggccagg tatggattca caatcaacgt
     2881 gtagacaccg tcgcggtcca ggacgaccgt ctccatcgcg gatggattcg cgccgatgcc
     2941 agtttcggac gctaatgcgg tgtcgtccgg gccgagcacc tgcattcggc ccgtgcagca
     3001 aggcaacgcg ccgaggtcgg aggctgtcac gcgtgcgacg atgcgctggt ctcgatggcc
     3061 ttcgaacgtg agccgagcaa cctgccccgg ttcgcggaag ctgatcgttc gctcggtatc
     3121 caggtcgatc gagtcttcga tgaccggcgc tccgccgagg atgcgcgctt cctgcacccg
     3181 ctccaggtta ccgtcaacgt cgattgacgc gaagagcagg tagctgggat cggtcagctc
     3241 gatcggtgcg tcgtacaact gtggcaacgg gttggccggg aagctccaaa tataggtgcg
     3301 atcaacccga tccttcccct gcgtcgtcag cgacacccgc gcgtcggtcg tgccgttgct
     3361 gtcgacggtc gcggtcgtct tgaactggcc gtagtcgtcg atgtcgtcgc cggtcttgac
     3421 gaagtccggc ttgagtgttt cgtcgaacgt cccgtccccg tcgcgatcaa cctccagccg
     3481 gagcttgccg acggaccgcg cggtgtcgcc cttgagccga agctgcgcgt tgaccggcgc
     3541 cgagacgacg aagatggcgc gtcccttgac ctcgtcatct ccaccgaaca atcccccacc
     3601 gctctcgttg acggagttga cggcgatgaa cgcgggaccc ggatcagtca cacggaggtg
     3661 ggccgtgtag ccgccgtggt cgtagtggaa tgtgtgcgtg gcactaggaa agcgcgcgtc
     3721 gggcgcgaag acgagcacat cgtcctcgta tccggtcgtg ccgacgatgc ctccaccggg
     3781 cgaagggaat ccggcgaccg agccattccc ttcgacgtat ccatacaacg gcccggtgac
     3841 gtggatcgac gctgagagct cagcattctg gattcgggac aatacgccgg cgcaggagac
     3901 ggtgcctgct acgatcgcga gcgcgacgat gccggcgcgg aacgttcgcc tatgcgccgt
     3961 tcgccagctt tgcagatgct gtacgacgat tgagacagtg gtgctcggcg ctacggatgg
     4021 cgttgtggga tgagcgggcg tcgcttcgcc ttgcgcctcc tcgctttgac gcagcgctcc
     4081 gatcgctggg acgatcaccc aggcgacaag gccggcgaac aggaaattgc ggatatcgga
     4141 atctgcatcg gtgatcacca tgacaacgta gaggacggct gtcacggcaa caacggcagc
     4201 tgccggccgg ctgtctaatc gccccgcagc aaacatcgcc gacaacgtca gggcggccgg
     4261 agccagcgca tagccgagca cggcattgac gctgccaatc gtcacgacga tcgcgatcga
     4321 acacccgaat acgaagccga agccgaccag cgccgtgcca cggacaccga gcatcttcgc
     4381 cagccagagc ggcccaccgg ccacgacgac gagcagcagg atgccaacca gcgcgaccgg
     4441 caggaagttc cgcagtccaa cgtcgtcgga cgcgctccac agcagca
//
LOCUS       DASUGJ010000370         4215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1725802, whole genome shotgun sequence.
ACCESSION   DASUGJ010000370 DASUGJ010000000
VERSION     DASUGJ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725802"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1931
                     /locus_tag="VFV93_08230"
     CDS             <1..1931
                     /locus_tag="VFV93_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5165368.1"
                     /translation="ISLGTFHAFCARVLRQYGSAIEIDPRFVIYDDSDQMSAVKQAME
                     SLDINTKSFSPRAVLSTISKAKSHNTGPTSYANDVESYFEEVVARVYPVYQQTLKRRR
                     ALDFDDLLLSTVRLLQQSEETVEALRQRHQYILVDEYQDTNQIQYLLVKELSQFHRNL
                     CVVGDPDQSIYGWRAADIRNILTFKQDYPDAVEIHLEENYRSTPQILSAADSVIRENT
                     QRIDRRLRTSNQAGDKIIVNECFDEAVEAKFVVDEIRRLTDQVGYKGRDIAVLYRTNW
                     QSRPIEEALIRAGIPYQLIGGTRFYERKEIKDILALLRLIANPDDSAAFERVVGEFPI
                     GDGIGAKSLSDLERWSQGKQVGLGASLDALGEAGGPTLSSRALKLLQAFNRDITALRD
                     AEPNLTLSELFDRALEETGYQGYFESGDPEMADRWENLQQFRANLERFDDEEPETRLM
                     TFLSEVALVSDADTVGDEADKVTLITLHAVKGLEFAVVFLTGIEEGLLPHQRSVQENP
                     AMLEEERRLFYVGITRAKERLYLTHAFRRSRFGSFEPSVPSSFLAAIPAEVLHNARPK
                     PDARKPAAKRLFDPAPAPVAPTWTRVSEGQTVFHTRFGDGIVTNVIDKGDDQEVTINF
                     KRHGEKRLIASMANLTVD"
     gene            1956..>4215
                     /gene="ppdK"
                     /locus_tag="VFV93_08235"
     CDS             1956..>4215
                     /gene="ppdK"
                     /locus_tag="VFV93_08235"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX5165369.1"
                     /translation="MASWVRAFDEQSEGADRDLLGGKGANLAEMTRLGLPVPPGFTVT
                     TEACRAYLASDEQLPDGLWEQVLAGVKQIEQRMGRELGGRDNPLLVSVRSGAKFSMPG
                     MMDTILNLGLNDESVHGLAASADARFAWDCYRRLIQMYAKVVLDVDSELFEDALDALK
                     RRTGVTLDYQLPAETLQELVGTFKQIVERKAGHPFPTDPYDQLRGAITAVFKSWNNRR
                     AIAYRNQNRIPHDLGTAVNVQSMVFGNLGADSATGVAFTRNPSTGETGIFGEFLVNAQ
                     GEDVVAGVRTPQPMAEMASLSAFQQAYAEFNDIASRLEKHYRDMQDVEFTIQNGRLFM
                     LQTRTGKRTGQAAVNVAVDMVGEGLISEQEAVQRVEPAQLDQLLHPMIDPAYTPDVLA
                     VGLPASPGAATGAIVYDADEAKLRGDEGERVILVRVATSPEDFHGMVSAQAVLTARGG
                     MTSHAAVVARGMGKPCVVGCGALDIDYASGTIQVDGRTLMKGDYITIDGATGRVMAGE
                     VPTIEPNVGGSVATLLGWADKYRRLGVRANADTPHDAGVAREFGAEGIGLCRTEHMFF
                     EGERILAMREMILASTPQERRAALDKLLPIQQSDFAGIFREMDGLPVTIRTLDPPLHE
                     FLPHTEGEMTALANELGVSVETIRTKVTALHEANPMLGHRGCRLGISYPEITEMQARA
                     ILQAAVECAKDGITVIPEIMIPLVADVKELERQRAVVEQVAGEVLGASGQQVDYSIGT
                     MIELPRAALTAGQ"
BASE COUNT          790 a         1426 c         1328 g          671 t
ORIGIN      
        1 ggatctcgct cggcacgttc cacgccttct gcgcccgcgt cctgcgccag tacggcagcg
       61 cgatcgagat cgacccgcgc ttcgtgatct acgacgatag cgaccagatg agcgccgtca
      121 agcaggcgat ggaatcactc gacatcaaca ccaagtcctt ctcgccgcgc gccgtcctca
      181 gcacgatctc caaagccaag agccacaata ccggcccaac ctcgtacgcc aacgacgtcg
      241 agagctactt cgaggaggtc gtcgcccgcg tctacccggt ctaccagcag acgctcaagc
      301 gtcgtcgcgc gctcgatttc gacgacctgc tattgagtac cgtccggctg ctgcagcagt
      361 cggaagagac ggtcgaggcg cttcgccagc ggcatcagta catcctggtt gatgagtatc
      421 aggacacgaa ccagattcag tacctgctcg tcaaggaact gagccagttc caccgcaacc
      481 tctgcgtcgt cggcgacccg gaccagtcga tctacggctg gcgtgcggcc gacatccgca
      541 acatcctgac gttcaagcag gactacccgg acgcggtcga gatccacctt gaggagaact
      601 accgctcgac cccgcagatt ctctccgccg ccgactcggt catccgcgag aacacccagc
      661 gcatcgaccg gcggctgcgg acctcgaatc aggccggcga caagatcatt gtcaacgagt
      721 gcttcgacga agccgtcgag gcgaagttcg tcgtcgatga gatccggcgt ctgaccgacc
      781 aggtcggcta caagggccgc gacatcgccg tcctctaccg caccaactgg cagagccggc
      841 cgatcgagga ggcactgatt cgggccggca tcccgtatca actgatcggc ggcacgcgtt
      901 tctacgagcg caaggagatc aaggacatcc tcgccctgct gcggctgatc gcgaatcctg
      961 acgatagtgc cgccttcgag cgggtcgtcg gcgagttccc aatcggtgac ggaatcggtg
     1021 ccaagtcgct ctccgacctc gaacgctggt cacagggcaa acaggtcggt cttggggcga
     1081 gcctcgacgc gcttggcgaa gcaggcggcc cgactctgtc gtcacgcgcg ctgaagctgt
     1141 tgcaggcgtt caaccgcgac atcaccgccc tgcgcgacgc cgagccgaat ctgacactct
     1201 cggagctgtt cgaccgggca ctcgaagaga ccggctacca gggctacttc gagagcggcg
     1261 atcctgagat ggccgatcgc tgggagaatc tgcaacagtt tcgtgccaat ctggaacggt
     1321 tcgatgacga ggagccggaa acgcggctga tgaccttcct cagcgaggtc gcgctcgtct
     1381 cggacgccga cacggttggc gacgaggccg acaaggtcac gctgatcacc ctccacgccg
     1441 tcaaggggct ggagttcgcc gtcgtcttcc tgaccggcat cgaggaaggg ctgctccccc
     1501 accagcgctc ggttcaggag aaccccgcca tgctggagga ggagcggcgt ctcttctacg
     1561 tcggcatcac ccgcgccaag gagcgcctct acctgaccca cgccttccgc cgttctcgat
     1621 tcggcagctt cgagccgtcg gttccatcct cctttctggc tgcaattccg gccgaggtgc
     1681 tgcacaatgc caggccaaag ccggacgcgc gtaagccagc cgcgaagcgt ctcttcgacc
     1741 ccgcaccggc accggtcgct ccgacctgga cacgggtgtc ggaaggacaa accgtcttcc
     1801 acacgcgctt cggcgacggc atcgtgacca acgtgatcga caagggcgac gatcaggaag
     1861 tgacgatcaa cttcaagcga catggagaaa agcgactgat cgccagcatg gcgaacctga
     1921 cagtcgatta ggggatgcag ggacaggggg acagggtggc aagctgggta cgtgcgttcg
     1981 acgaacagag cgaaggcgcc gatcgcgatc tgctcggcgg taagggcgcg aatctggccg
     2041 agatgacccg gctgggtctg ccagttccgc cgggcttcac cgtcacaact gaggcctgcc
     2101 gtgcctatct cgccagcgat gagcagttac cggacggtct ctgggagcag gtgctggcgg
     2161 gcgtcaagca gatcgagcag cgcatgggcc gcgagcttgg cgggcgcgac aacccgctgc
     2221 tcgtctcggt ccgctccggc gcgaagttct cgatgcctgg catgatggat acgatcctga
     2281 acctcgggtt gaacgatgag agcgtccacg gactggccgc cagcgccgac gcccgcttcg
     2341 cctgggactg ctatcggcgg ctgatccaga tgtacgccaa ggtcgtcctc gacgtcgaca
     2401 gcgagctgtt cgaggatgcg ctggacgccc ttaagcgccg gaccggcgtc acactcgact
     2461 accagcttcc agccgagacg ttgcaggaat tggtcggcac cttcaagcag atcgtcgagc
     2521 gcaaggccgg ccatccgttc ccaaccgacc cgtacgacca gcttcgtggg gcgatcacgg
     2581 ccgtcttcaa gtcgtggaac aaccgccggg cgatcgccta ccgcaaccag aaccgcatcc
     2641 cgcacgatct cggcacagcc gtcaacgtcc agtcgatggt cttcggcaac ctcggcgccg
     2701 acagcgcgac tggcgtcgcc ttcacccgca atccttcgac cggtgagacg ggcatcttcg
     2761 gtgagttcct cgtcaatgct cagggagagg acgtcgtcgc cggcgtccgg acgccgcaac
     2821 cgatggccga gatggcgtcg ctgtccgcgt tccagcaggc ctacgcagag ttcaacgaca
     2881 tcgcctcgcg gctggagaag cactaccgcg acatgcagga tgtcgagttc acgatccaga
     2941 acggccgcct gttcatgctt cagacgcgga ccggcaagcg caccggccag gcggccgtca
     3001 acgtcgccgt cgacatggtt ggcgagggac tgatcagcga gcaggaagcc gtccagcgcg
     3061 tcgaaccggc ccagctcgac cagctcctgc acccgatgat tgatcccgcc tacacgcccg
     3121 acgtgctcgc ggtcggcctt ccggccagcc ccggcgcggc gaccggcgcg atcgtctacg
     3181 atgccgatga ggccaagcta cgtggcgacg agggtgagcg ggtcatcctc gtccgcgtcg
     3241 cgacatcgcc ggaggacttc cacgggatgg tctcggccca ggccgtcctg acagcccgtg
     3301 gcgggatgac ctcgcacgcg gccgtcgtcg cccgtggcat gggcaagccg tgtgtcgtcg
     3361 gctgcggtgc gctcgatatc gactacgcca gcggtacgat ccaggtcgat ggtcggacgt
     3421 tgatgaaggg cgactacatc acgatcgacg gcgcgaccgg ccgggtcatg gccggcgaag
     3481 tgccgacgat cgagccaaac gtcggcggca gcgtcgcgac attgctcggc tgggccgaca
     3541 agtaccgccg cctcggcgtc cgcgccaacg ccgacacccc gcacgatgcc ggcgtcgccc
     3601 gcgagttcgg agccgagggc atcggcctct gccggactga gcacatgttc ttcgagggcg
     3661 agcgcatcct cgccatgcgc gagatgatcc tcgcctcgac gccgcaggag cgccgcgcgg
     3721 cgctcgacaa gctcttgccg atccagcagt ccgacttcgc cggcatcttc cgggagatgg
     3781 acggcctgcc ggtcacgatc cggacgctcg atccacctct gcacgagttc ctgccgcaca
     3841 ccgaagggga gatgacggcg ctggcgaacg agctaggcgt cagcgtcgag acgatccgca
     3901 ccaaggtcac cgccctccac gaggccaacc cgatgctggg ccaccgcggc tgccggctgg
     3961 gcatctccta cccggagatt accgagatgc aggcccgcgc catcctccag gcagctgtcg
     4021 agtgcgcgaa ggacggcatc accgtcatcc ccgagatcat gatcccgctg gttgccgacg
     4081 tgaaggagct ggaacgccag cgcgccgtcg ttgagcaggt cgccggcgag gtcctcggcg
     4141 catcaggcca gcaggttgac tacagcatcg gaacgatgat cgagctgcca cgcgcggcac
     4201 tgacggccgg ccaga
//
LOCUS       DASUGJ010000371         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_336282, whole genome shotgun sequence.
ACCESSION   DASUGJ010000371 DASUGJ010000000
VERSION     DASUGJ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336282"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="VFV93_08240"
     CDS             <1..178
                     /locus_tag="VFV93_08240"
                     /inference="COORDINATES: protein motif:HMM:NF021855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX5165370.1"
                     /translation="DATHDSLTIEVTGPTDKIESLLAMIRPYGIKEVARTGAVAMTRG
                     ANTNGLTLLRDQVA"
     gene            208..1218
                     /gene="ilvC"
                     /locus_tag="VFV93_08245"
     CDS             208..1218
                     /gene="ilvC"
                     /locus_tag="VFV93_08245"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX5165371.1"
                     /translation="MATIYYEKDANADLLKDKKIAVLGYGSQGHAHAQNLKDSGLDVR
                     IGLYEGSKSWEKAEADGLKVMTSADAAAEADVIMVLVPDHIQKRLYNEAIAPNLSEGN
                     VLMFAHGFNIHFGHIQPPANVDVTMIAPKGPGHIVRDMYTEGIGVPALLAVHQDVSGQ
                     AKDLALAYALALGSAKAGVIETTFKEETETDLFGEQAVLCGGVSHLITTAFETLVEAG
                     YQPEIAYFEVLHEMKLIVDLMYQGGIKYMRYSVSDTAEYGDYTSGPRVIDDRVRATMR
                     QVLGDIQSGEFANEWIGENEAGRERFLNMRRDANVHQIETVGDELRSMMPFLQKKAVP
                     TN"
     gene            1412..>2899
                     /locus_tag="VFV93_08250"
     CDS             1412..>2899
                     /locus_tag="VFV93_08250"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5165372.1"
                     /translation="MTDSSTTDGTVRRILDNGLTAIVRSERAAPIGTFWIWYRVGGRN
                     EVPGITGVSHWAEHMLFKGTERYPKGSIFRTVTANGGSLNGFTWLDYTTYFETLPVDR
                     IDVAFDIEADRMVNARFDPDEVASERTVIISERQGNENSPTFLLNEELTAASFKAHPY
                     GQGVIGYMSDLRTMTRDDLYNHYQTYYRPNNAVAIFVGDLETGDALERIERFFGGNER
                     GPDIPDVRTVEPEQYGERRVTVRRPAPNRVVSLAHRGVAASHPDAPALIVLDAVLSGG
                     KPMSFGGGASMGRSSRLYRALVATGLTASAGSSFALSIDPLLFGVSATLRPESDSEHV
                     EQVIIGEIERLAREPVGQDEFERALKQIRAQFTYSQQSVSSKAYLLGSLAMVAPERTP
                     QSLIDAVLAVTSDDVQRVAATYLTWERRTTGWLIPEEFNGTTVAGAPATTVAARYVPD
                     EDDVALDTSMPALAETRLANGLRVMTLEATDRDPNGPVVVRLRIPG"
BASE COUNT          544 a          939 c          961 g          455 t
ORIGIN      
        1 cgacgcgacg cacgactcgc taacgatcga agtgaccggc ccgaccgaca agatcgaatc
       61 gctgctggcg atgatccggc cgtacggtat caaggaagtt gcgcgcaccg gcgcggtcgc
      121 gatgacgcgc ggcgcgaaca caaatggcct gacgctgttg cgcgatcagg tggcctgaca
      181 gggccgcagg ggagagggag acgactggtg gcaaccatct actacgagaa ggacgcgaac
      241 gccgaccttc tcaaggacaa gaagatcgcg gttctcggct acggcagcca gggccacgcc
      301 cacgcgcaga acctgaagga ctccggtctc gacgtacgaa tcggcctcta cgagggcagc
      361 aagtcgtggg agaaggccga ggccgatggc ctgaaagtga tgacgagcgc tgacgccgcc
      421 gccgaggcgg atgtcatcat ggtcctcgtg ccggaccaca tccagaagcg gctctacaac
      481 gaggcgattg cgcccaacct gtccgagggc aacgtcctga tgttcgccca cggcttcaac
      541 atccacttcg gccacatcca gccgccggcc aatgtcgatg tgacgatgat cgcgccgaag
      601 ggccccggtc acatcgtgcg cgacatgtac accgaaggga ttggcgttcc ggccctgctg
      661 gccgtccacc aggatgtctc aggccaggcg aaggacctcg cactggccta cgcgctggcg
      721 ctcggctcgg ccaaggctgg cgtgatcgag acgaccttca aggaagagac agagaccgac
      781 ctgttcggcg agcaggctgt cctctgcggg ggagtttcgc acctgattac gaccgccttc
      841 gagacgctgg tcgaggccgg ctaccagccg gagatcgcgt acttcgaagt gctgcacgag
      901 atgaagctga tcgtcgacct gatgtaccag ggtggcatca agtacatgcg ctactcggtc
      961 tccgacacgg ccgagtacgg cgactacacc tccggcccgc gcgtcattga cgaccgggtc
     1021 cgggcgacga tgcgccaggt actcggcgat atccagtccg gcgagttcgc caacgagtgg
     1081 atcggcgaga acgaggccgg ccgtgagcgc ttcctcaaca tgcgccgcga cgccaacgtc
     1141 caccagatcg aaaccgtcgg cgatgagctg cgctcgatga tgccgttcct gcagaagaag
     1201 gcggttccga cgaactgaca gtttcggaaa accccacccc caaccccctc ccctgagaag
     1261 agagggggct tttggccgta cgaaatctga gccccctctc ttctcagggg agggggttgg
     1321 gggtggggtt accgcctgcc aacagtcgcg cgcgcctcta tactgcaagc gctgccggtt
     1381 gggtagcgac tggctggacg gagggcgccg catgaccgat agcagcacga ccgacgggac
     1441 ggtccgcagg atactcgaca acgggctgac cgcgatcgtc cgctccgagc gagcggcacc
     1501 gatcgggacg ttctggatct ggtatcgggt cggcggccgg aacgaggtgc ctggtatcac
     1561 cggtgtctcg cactgggccg agcacatgct cttcaagggg acggagcgtt acccgaaggg
     1621 ttcgatcttc cggaccgtca ccgccaacgg cggcagcctc aacgggttca cctggctgga
     1681 ctacacgacc tacttcgaga ctctgccggt cgaccggatc gacgtcgcat tcgacatcga
     1741 ggcagaccgg atggtcaacg cgcgcttcga ccccgacgag gtcgccagcg agcggaccgt
     1801 catcatctcc gaacgccagg gcaacgagaa ctcgccgacg ttcctgctca acgaggagtt
     1861 gacggccgcc tcgttcaagg cgcatcccta cgggcagggc gtcatcggct acatgtccga
     1921 cctgcgcacg atgacccgcg acgatctcta caaccactac cagacctact atcggccaaa
     1981 caacgcggtc gcgatcttcg tcggcgacct ggagaccggc gacgcgcttg agcggattga
     2041 gcgcttcttc ggcggcaacg aacgtgggcc ggacatcccc gacgtgcgca cggtcgagcc
     2101 ggagcagtac ggcgagcggc gggtgacggt ccgacggccg gcgccgaacc gggttgtgtc
     2161 gctggcccat cgtggcgtcg cggcctcgca cccggatgcg ccggccctga tcgtcctcga
     2221 tgccgtcctc tccggcggca agccgatgag cttcggtggc ggagcctcga tggggcgttc
     2281 gtcgcggctc taccgcgcgc tggtcgcgac cggcctgacg gcctcggcag gcagctcgtt
     2341 cgcgctctcg atcgacccgc tgctgttcgg cgtcagcgcg acgttgcggc ccgaatcgga
     2401 cagcgagcac gtcgagcagg tcatcatcgg cgagatcgag cgactggcac gtgagccggt
     2461 cggacaggac gagttcgaac gggcgctgaa gcagatccgt gcccagttca cctacagcca
     2521 gcagagtgtc tcaagcaagg cgtatctact cgggtcgctg gcgatggttg cgccggaacg
     2581 gacgccacag tcgctgatcg acgccgtgct ggccgtgaca tctgacgacg ttcagcgcgt
     2641 cgctgcaacc tacctgacct gggagcgacg gacgaccggt tggctcatcc ccgaggagtt
     2701 caatggaacg acggtggcgg gtgcaccggc cacgacggtt gcggcgcgat atgttcccga
     2761 cgaggacgat gtcgcgctcg acacgtcgat gccggcactg gccgagacgc ggctcgcgaa
     2821 cggcctgcgg gtgatgacgc tggaggcaac cgatcgcgac ccgaacggtc cggtcgtcgt
     2881 ccgcctgcgc atccctggt
//
LOCUS       DASUGJ010000372         4749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_336361, whole genome shotgun sequence.
ACCESSION   DASUGJ010000372 DASUGJ010000000
VERSION     DASUGJ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336361"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..131
                     /locus_tag="VFV93_08255"
     tRNA            59..131
                     /locus_tag="VFV93_08255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92..94,aa:Arg,seq:tct)
     gene            398..1213
                     /locus_tag="VFV93_08260"
     CDS             398..1213
                     /locus_tag="VFV93_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165373.1"
                     /translation="MISFSSLARSGRVVVAVSLGLVLLAGGCSFEDFFAGSESRDDSS
                     QRETAPPTPTSTVPSTFTPWIYVPKTATRTATPTRTPTPRPTATPSPTPSPTPVVNLL
                     GSLPSEEDVPDGMRLEREEATLSAEQVADEVEGPDRAEYLRLLDDWDFRGGSLREYQL
                     PSPGVGEFLRDLLGLEARGLEFGSEANALAAIKYQREFARNRAGWDLHDADVEQLGDA
                     SFALTGTADYDGTEVSVAAVFVQEGNRVYRFVGVGGLQKPFDETLKIAKRALS"
     gene            1272..2156
                     /locus_tag="VFV93_08265"
     CDS             1272..2156
                     /locus_tag="VFV93_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5165374.1"
                     /translation="MERPGFVSRIWYPVDRARRTRLGGLLERAIRDFTGDHASVYAAA
                     ITYYLLLSLFPLLVIVVSVIGLLARDPSFQAQVVDQISTLLPGGSGVDEQVEQIVAGI
                     ARPRSGVVGLVGLLGALWAASGVFGAVRKALNNAFDVPVGQSFIRGRLHDLLSVIAVV
                     LMALASTTLTATLAFARAWVSRWVDSPLTNVGWGLVYLLLPLALSFTFFVLLYWLIPN
                     QRLYLRDLWIGALIAGLGFELAKALFTVYLANFANFTEVYGALGSMIALLVFVYIVAN
                     IAIFGAEVASEIAKERAG"
     gene            complement(2163..4136)
                     /locus_tag="VFV93_08270"
     CDS             complement(2163..4136)
                     /locus_tag="VFV93_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX5165375.1"
                     /translation="MVRLSLTVAVTLALLLAACGSDDNGSDGADASPTTASATATASE
                     PGASPTATDAASPVASPTTSPTTAAGTEAPYELVATWGEPALLAQPFDVAVTPDGGLV
                     VANLLYGRLVFLDADGKPTNTWDIDIFSQVAVDGAGNIYVRDSRSRGLTKYDSAGNEL
                     ATLELGTEQLQFSLNDMAASEDGYLYATSGEGRSSSSTPSPYRGVYVFEPAGERIAEW
                     AAPLAYHPQAVTVGPDGVVYVSAVEKDAEHMPVRDVLIRIDPDSYSPNDWLDSQIALP
                     EGTIIDALAATPSGELYALRESDPNRGNPTPTTIIRMDTTGTVLAEWTVDDVDGSGLP
                     GATGIAAAPGGGVYLTDGLNHRVLKLDPDGNLLVEYGEPNPATISIPTGIMVGADGTV
                     YVRDSGFDRILTFSPEGEPAGIVDVPGEYRGFAFTSPPDVAVGADGVVWATQSPPTNV
                     YGLTREGDIVHKWTKTVIDRPGDHPLVFGPGSMAVTADNTLLISNDLPPIVFEFSTDG
                     EILDEWTLTDDKQSRFHAVTVANGTRYAMINDENDDPATGFTIEVLRLGEDGALETVL
                     SIPIEVDAGSGNNAFIPVDCAIDRDGNIFLADLFNRQILKYGPDGNALGRWSLEGDFE
                     RPNMFISIAIGDDGRIYVADGELQQVFVYAPVG"
     gene            4485..>4749
                     /locus_tag="VFV93_08275"
     CDS             4485..>4749
                     /locus_tag="VFV93_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165376.1"
                     /translation="MRTTGNRQAGALLLLALIAAACSGPDDARSPTERATTDAVVSPT
                     VAAPAPETPTQVATQPGSTTPTATTAPVVATPSPTAAPEGAVCD"
BASE COUNT          699 a         1491 c         1663 g          896 t
ORIGIN      
        1 ctccgcgttc tggcgcctct gtcgacacac tcgctacccg gccacgaaag gcctttgagc
       61 ccccgtagtt cagtggatag aacgacggcc ttctaagccg tatgtcgcag gttcgattcc
      121 tgccgggggt accgctgggc cggttggtgg tggttcgcct ggggcgtcgt agtcgcgatt
      181 ccatcttccg gcgatctgtg ctagagtgta cgtacggaca ccggagggat cggtatcggc
      241 tcgggccacc ggcgatggcc tgttcctccc actgtccgga ttctttccct gtaacgaaca
      301 cagcttccat cggcttgtca taagcactgc gtatccacgg tatgtgggtt gcgttgctgg
      361 gtcgtgatcg gcgttcgtgt tcgcgtcagg ggcttccatg atttcgttct catcgcttgc
      421 tcggtcgggt cgggtcgttg tcgccgttag cctcgggctg gtgctgctgg ccggcggctg
      481 cagcttcgag gatttcttcg ccggctcgga gagtcgcgac gattcgagcc agcgggagac
      541 cgcgccgccg acgccgacat cgacggtgcc gtcgacgttt acgccgtgga tctatgtccc
      601 gaagacggcg acgcggacgg ccaccccgac tcgcacaccg acgccgcggc caacggcaac
      661 gccgtcgcca acaccgtcgc caacgccggt tgtgaacctg ctcggctcgc tgccgtcgga
      721 ggaggatgtc ccggacggga tgcggctgga gcgcgaggag gcaacgctgt cggcggagca
      781 ggtggccgac gaggtcgaag ggccggatcg ggccgagtac ctgcggctgc tggacgattg
      841 ggatttccgg ggcgggtcgt tgcgcgagta ccagttgccg tcgccgggtg tcggcgagtt
      901 cctgcgcgac ctgctgggac ttgaggcgcg cgggctggag ttcggctcgg aggccaacgc
      961 gctggccgcg atcaagtacc agcgcgagtt cgcccgcaac cgtgccggct gggaccttca
     1021 cgacgccgat gtcgagcagc ttggcgacgc gtccttcgcg ctgaccggga cggccgatta
     1081 cgacgggacc gaggtgtcgg tcgccgcggt cttcgtccag gagggcaacc gcgtctaccg
     1141 cttcgtcggt gtcggcggct tgcagaagcc gtttgacgag acgttgaaga tcgccaaacg
     1201 cgcgttgtcg taggcaggcc gcaccgcggg tggtgctgga ttcgctccgc ccgtcgcggt
     1261 aggctgccgg catggagcgg cctggattcg tcagccgaat atggtatccc gtcgatcgcg
     1321 cgcgacggac gcggctgggc ggtctgctgg agcgcgccat ccgcgatttc accggcgatc
     1381 acgcgtcggt ctatgcggcg gcgatcacct actacctgct cttgtcgttg tttccgctgc
     1441 tggtgatcgt cgtgtcggtg atcggcctgc tggcgcgcga cccatcgttc caggcgcagg
     1501 tcgttgacca gatctcgacg ctgctgccgg gcggctcggg tgtcgatgag caggtggagc
     1561 agatcgttgc cggcatcgcc cgtccgcgca gcggtgtcgt cggcctggtc gggcttctcg
     1621 gcgcgctctg ggcggccagc ggcgtcttcg gcgcggtccg taaagcgctc aataacgcgt
     1681 tcgacgtgcc ggtcgggcag tcgttcatcc gtggcagatt gcacgatctg ctgagcgtca
     1741 tcgccgtcgt gctgatggcg ctggcatcga cgacgctgac ggcgacgctg gcgtttgccc
     1801 gcgcctgggt cagccgctgg gttgacagcc cgctgaccaa cgtgggctgg gggctggtct
     1861 acctgttgct accgctggcg ctgtcgttca cgttcttcgt cctgctctac tggctgatcc
     1921 ccaaccagcg gctctacctg cgcgatctct ggatcggcgc gctgatcgcc ggtctcggct
     1981 ttgagctggc gaaggcgctc ttcaccgtct acctggccaa cttcgccaac ttcaccgagg
     2041 tctacggcgc gctcggcagc atgatcgcgc tgctggtctt cgtctacatc gtcgccaaca
     2101 tcgccatctt cggcgccgag gtcgcctcag agatcgcgaa ggagcgtgct gggtgagggg
     2161 attcacccca ccggcgcgta gacgaagacc tgttgcagct cgccatcggc gacgtagatg
     2221 cggccgtcgt cgccgatggc gatgctgatg aacatgttgg gccgctcgaa atcgccttcg
     2281 agcgaccagc ggccgagtgc gttgccgtcg gggccgtact tcaggatctg gcggttgaac
     2341 aggtcggcca gaaagatgtt gccgtcgcgg tcgatggcac agtcgaccgg gatgaaagcg
     2401 ttgttgccac tgccggcgtc gacctcgatc gggatgctca ggaccgtctc cagcgcgccg
     2461 tcctcgccca gccgcaggac ctcgatcgtg aagccggtcg ccggatcgtc attctcgtcg
     2521 tttatcatcg cgtagcgtgt cccgttcgcg acggtgacgg catggaagcg cgactgcttg
     2581 tcgtcggtca gcgtccactc gtcgaggatc tcgccatcgg tgctgaactc gaagacgatc
     2641 ggcggcaggt cgttcgagat gaggagcgtg ttgtcagccg tgactgccat cgagccgggg
     2701 ccgaagacca gtggatggtc gccgggccgg tcgataactg ttttggtcca cttgtgaacg
     2761 atgtcgccct cccgcgtgag tccgtagacg ttggttggcg gggattgcgt cgcccagaca
     2821 acgccgtcag cgcccactgc cacgtcaggc ggggacgtga aggcgaagcc gcgatactcg
     2881 ccgggcacgt ccacgatccc ggccggctcg ccttcagggg agaacgtcag gatccgatcg
     2941 aagccgctgt cgcggacgta gaccgtcccg tccgccccaa ccatgatgcc agtcgggatg
     3001 ctgatcgtgg ctggatttgg ctcgccgtac tcgaccagca ggttgccgtc ggggtcgagc
     3061 ttcaggacgc ggtggttgag gccgtcggtc aggtaaacgc cgccgccggg cgcggcggcg
     3121 atgccggtgg cgcctgggag gccggagccg tcgacatcgt cgaccgtcca ctccgccaga
     3181 acggtgccgg tcgtgtccat gcggattatc gtggtaggtg tggggttgcc gcggttcgga
     3241 tcgctctccc gcagggcgta cagctctccg ctcggcgttg ccgccagcgc gtcgatgatc
     3301 gttccctccg gcagggcgat ctgggagtcg agccagtcgt tcgggctgta gctgtcagga
     3361 tcgatgcgga tgaggacatc gcggaccggc atatgttccg cgtccttctc gacggctgag
     3421 acgtagacga cgccgtcggg gccaaccgtg acggcctgcg ggtggtaggc gagcggggcg
     3481 gcccactcgg caatccgttc gccggccggt tcgaagacgt agacgccacg gtatggcgag
     3541 ggggttgacg aactggagcg cccttcgccg ctggtggcgt agaggtagcc gtcttcgctg
     3601 gcggccatgt cgttcaggct gaactggagc tgttccgtgc cgagttcgag cgtcgcgagc
     3661 tcgttgccgg cggagtcgta tttggtcagc ccacgtgagc ggctgtcacg cacatagatg
     3721 ttgccggcgc catcgacggc gacctggctg aagatgtcga tatcccaggt gttcgtcggt
     3781 ttgccgtcgg catcgaggaa gacgagccgc ccatagagca ggttggcgac gaccagcccg
     3841 ccgtccgggg taacggccac gtcgaacggc tgggcgagca gggccggttc gccccaggtc
     3901 gcgacgagtt cgtacggcgc ctcggtcccg gcggcagtcg tcggtgacgt tgtcggggat
     3961 gcgacgggtg atgcggcgtc cgttgctgtc ggcgacgcgc cgggctcgga tgccgtcgcg
     4021 gtggcactgg cggtcgtggg ggaggcgtcg gcgccgtccg atccgttgtc atcggatccg
     4081 caggcggcca gcagcagggc cagcgtgacg gcgacggtga gactgagtcg gaccatggat
     4141 actccctttg aagatagatg cgggttggct gctgcgaccg ctgttcagaa gagcgaaggc
     4201 aagcggatgc actcagacac cggccaccga cccccgtctt gtcatccttc ggctcacgcc
     4261 tcaggatgac aaggggacgc cgcatgacaa aagggcgcgc tgcacattcc gcgaaggtct
     4321 gggcaattgt cgcatgccgg caagatattg cacaacagtc tcgcacatgg catgccggga
     4381 gtagtctctg agggcgcgac gatcctgccg ctcgaagtcc aattcacggc ctccggcaac
     4441 gccgtctgat aggctcttgc gtcatggctg gatggtggac gcgcgtgcga acgacgggca
     4501 atcggcaggc aggtgcgctg ctgctgctgg cgctcatcgc cgccgcctgt agcgggccgg
     4561 acgacgcgcg cagccctacc gagagggcca cgaccgacgc tgtcgtgtcg ccgaccgtcg
     4621 cggcgcccgc ccccgaaact cccacccagg tggcgacgca accaggctcc accacaccga
     4681 cagcgacgac cgcacccgtc gttgcgacgc catcaccaac tgccgcgccg gaaggcgccg
     4741 tttgcgaca
//
LOCUS       DASUGJ010000373         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_336394, whole genome shotgun sequence.
ACCESSION   DASUGJ010000373 DASUGJ010000000
VERSION     DASUGJ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336394"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2173)
                     /locus_tag="VFV93_08280"
     CDS             complement(<1..2173)
                     /locus_tag="VFV93_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5165377.1"
                     /translation="MSDRSRLAFERVEIRRMPGIEAGFAVDGLCPGINVICGPNASGK
                     TSLTRAVATLLWPRSTGASRSILHGTYALDGRSWKVEFDSGSARWQSDGQDADAPAID
                     AADGNDRYLLALHDLLRADNRDFAEAILRESTGGYDVGGAATALGYSPRPSSPIALKQ
                     RLDAAHAGEREARQAQEALRQQERERDELLGRQEQARAAGHRAQAIERALAVHRAQAT
                     LDEATRTLVAFPPVLFRLSGTEPEELKRLRQRQMEASQAVEQEQLGIVDARERQLATG
                     LTTPLPPAAIAGLRDRFATLQNDSRELRAAGERRTEAEAVVAAAREAIGPALIDDQIA
                     ALDPVQFNALAGLMQQSRDAHAKLEALSQIADWIPPLPQDTDQDDARDRLAIGARCLT
                     RWLREGSEPAIAGNRAALPLLIAAGVAVIESVLLGVLVHPALFVLLLVTVAFLVFGLR
                     QQSAPERDGAADWRAEYQRLGLPAPAAWTSDAVETLIEELLRRARRIQADQEARQRWQ
                     YVRDERPVAVQHAAEVERQVAAWCVRFSVSCVRDVAALHTLANAISSWQDARGRAASA
                     AALQRDAQARCDAALASINTTLLPFGYPAADGPAGVGSAIDDLTDRDRLWADAVADEQ
                     RAQRRLADRIEPLQQRCREELQAFFERLGLSDDDEPGLQALLDRLPDYREAALAVERA
                     GLEQEHAQAALGDAVALVAHSPASLAAELEEAHTQHATLDDI"
     gene            complement(2170..>2965)
                     /locus_tag="VFV93_08285"
     CDS             complement(2170..>2965)
                     /locus_tag="VFV93_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165378.1"
                     /translation="LDQSASRYGPVPLADLTRTPVAAWLLGHIHHPRAIGGANQPLVL
                     YPGSPLAMDPGETGSHGPWLLDLAPGRPVGCRQLPTTPIRYDRLPIALDAIERRDDLL
                     DQIASAVQQRLLEIAEDTGGLACVAFRLELTGRTPLHREMERHLRELQADFQPQQVGV
                     TGFVERVLDETHPAIDLDDLSRRNDPPGELARVLLALEADPAPPGYRQLLEDTLARLN
                     DLRRQRVYGGIAGDERPSLADARRHLLGEGYRLLDMLIMQREQVSV"
BASE COUNT          460 a         1033 c         1024 g          448 t
ORIGIN      
        1 cgatatcgtc aagcgtcgcg tgctgggtgt gagcctcctc cagctcggca gcgaggctgg
       61 cgggcgagtg cgctaccaac gcaaccgcgt cgccgagtgc cgcctgcgca tgctcctgct
      121 ccagcccggc acgttcgacg gccagcgccg cctcgcgata gtcaggcagg cgatcgagga
      181 gcgcttgcag tccaggttcg tcgtcgtcgc tcagacccag gcgctcgaag aatgcttgca
      241 actcctcgcg acagcgctgc tggagtggtt cgatccggtc ggcaagccgc cgctgagcgc
      301 gctgctcgtc ggcgacggca tcggcccaca gccgatcgcg gtcagtgaga tcatcgatgg
      361 cgctgccaac gccggccggg ccatcggcag cgggataccc gaacggcagc agcgtcgtgt
      421 tgattgaggc cagggcggcg tcacaccgcg cttgagcatc gcgctgaagc gccgccgcgg
      481 acgccgcacg accacgcgcg tcctgccagc tgctgatggc gttggccagc gtgtgaaggg
      541 cggcgacatc acggacgcag gagacactaa accgcacgca ccacgcggcg acttgccgct
      601 cgacctctgc ggcatgttgg acggcaaccg gtcgctcgtc gcggacatac tgccagcgtt
      661 gacgagcttc ctgatcggcc tggatacgtc gggcccgccg gagcagctcc tcaatcaacg
      721 tttcaacggc atctgacgtc cacgcggccg gagccggcag accgagccgc tggtattcgg
      781 cgcgccagtc cgcggcccca tcacgctccg gcgcagactg ttgccgcagg ccaaagacaa
      841 ggaacgcgac cgtgaccagc agcaggacga acaacgcggg atggaccagc acgccaagca
      901 gcaccgactc gatgacagcg acaccggccg cgatcagcag tggcaacgcc gcgcggttcc
      961 ccgcgattgc cggttcgctc ccctcacgga gccaacgggt caggcagcgc gcaccgattg
     1021 ccagccggtc gcgcgcgtcg tcctgatcgg tgtcttgggg taacggtgga atccagtcgg
     1081 cgatctggct cagtgcttcg agcttcgcgt gggcgtcgcg cgattgctgc atcagcccgg
     1141 ccagcgcgtt gaactggacc ggatcgagcg ctgcgatctg gtcgtcgatc agcgccggtc
     1201 cgatcgcctc acgggcagcc gcgacgaccg cttcggcctc ggtgcgccgt tcgccagcgg
     1261 ccctgagttc gcggctatca ttctgcaacg tggcgaaacg gtcgcggagg ccagcgatcg
     1321 cggcgggcgg caagggtgtc gtcaagccgg tcgcaagctg gcgttcccgc gcgtcgacga
     1381 tcccaagctg ttcctgctcg accgcctgac tggcctccat ctgccgttgg cgcaagcgct
     1441 tcagttcctc tggctcggta ccactgagac ggaagaggac gggcgggaag gccaccaacg
     1501 tgcgagtcgc ctcatccagc gttgcctggg cccggtggac ggccagcgcc cgttcaatcg
     1561 cctgcgcgcg atgtccggcc gcacgtgcct gctcctgcct gcccagcagc tcgtcgcgct
     1621 cgcgctcctg ctgacgaagt gcctcctgcg cctgccgcgc ctcgcgctcg ccggcgtgag
     1681 ccgcatcgag ccgctgcttg agcgcgatcg gcgacgacgg ccttggactg tagccgagcg
     1741 cggtcgcggc cccgccgacg tcgtagccac cggtcgattc gcgcagaatc gcctcggcga
     1801 agtcgcggtt gtcggcgcgc agcaggtcat ggagcgccag caggtagcgg tcgttgccat
     1861 cggcagcgtc gatcgcgggg gcgtcggcgt cctgcccgtc ggactgccag cgcgcgctgc
     1921 cgctgtcgaa ctcgaccttc cacgatcgac catcgagcgc gtaggtgccg tggaggatcg
     1981 agcgcgacgc gccggtcgag cggggccaga gtaatgtcgc gaccgcccgc gtcagcgagg
     2041 tcttgccgga ggcgttcggc ccgcagatga cattgatgcc ggggcagaga ccatcgacgg
     2101 cgaagccagc ctcgatcccc ggcatccgcc ggatctcgac ccgctcgaac gccagtcgtg
     2161 accggtcact cacacgctca cctgctcccg ttgcatgatc agcatgtcga gcagccggta
     2221 gccctcgccc agcaggtggc ggcgggcgtc ggccaggctg gggcgctcgt caccagcgat
     2281 gccgccgtag acgcgctgac gccgaaggtc gttgaggcgc gccagtgtgt cctccagcag
     2341 ctgccggtaa ccgggcggcg cggggtccgc ctccagcgcc agcagcacgc gcgccaactc
     2401 gccgggcggg tcgttgcgcc gcgagaggtc gtccaggtca atcgccggat gggtttcatc
     2461 cagcactcgc tcgacgaagc cggtgacgcc aacctgctgc ggctggaaat cggcctgcaa
     2521 ctcccgcagg tgacgctcca tctcgcggtg cagcggagtc cggccggtca gctccagccg
     2581 gaaggcgacg caggccaggc cgccagtgtc ttctgcgatc tccagcaagc gctgctggac
     2641 tgcgctggcg atctggtcca gcaggtcgtc gcgcctctcg atggcatcca gcgcgatcgg
     2701 cagccggtcg tagcgaatcg gcgttgttgg cagttgccgg caaccgactg gccggccggg
     2761 agccagatcc agcagccacg ggccgtgcga accggtctca ccgggatcca tcgccagcgg
     2821 cgaaccgggg tagaggacca gcggctgatt cgcgccaccg atcgcacggg gatggtggat
     2881 atggcccagc agccaggcag cgaccggcgt gcgcgtcagg tccgcgagtg gaaccggccc
     2941 gtagcggctg gcggactggt cgagg
//
LOCUS       DASUGJ010000374         5035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_892240, whole genome shotgun sequence.
ACCESSION   DASUGJ010000374 DASUGJ010000000
VERSION     DASUGJ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892240"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1472)
                     /locus_tag="VFV93_08290"
     CDS             complement(72..1472)
                     /locus_tag="VFV93_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165379.1"
                     /translation="MQVGDESFSSFQPAEIPVAGDQDDLIGPVYATFDVLRSYLPFPV
                     GGWITQRIDRSATAVDDPALASYAVTVATVDPVTNHSIATPFWEFMTSTGTIYENGAY
                     HEAQLFADPLYATGRPIAEPYWATVKVGGSYRDVLLQCFERRCLTYTPDNPDGWKVEA
                     GNVGRHYLAWRSAQPEVDHDSVIVYRTLVSTGDGNRNQEIFSVRADGTAERNLSNNSD
                     SDFVPELSPSGRYIAYLHAPFQKPHELWLMNADGSNQRPHPGMEYMRSVGLDPYIGGG
                     FAWSPDETYLAVSSFGHGDNYNIFLLANDGSGAQQVTHFSNNQHVTEIDWSPDGTSLV
                     FATVSFTDIGTGPPTLYTIGVDGTGLRQLSTNTSRCDQPDWSPDGSKIACSWGQIIVM
                     NSDGSDWHIITDMPQGAWSPSWSLDGASIAFEAMDGFGGIASGVAVVASTGGTVSWIA
                     EPGTGSNHPSWGPAIP"
     gene            complement(2001..2780)
                     /locus_tag="VFV93_08295"
     CDS             complement(2001..2780)
                     /locus_tag="VFV93_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5165380.1"
                     /translation="MVDQTRADRDVRRSDEQYTPIASAYVTSAIHAQGQDLPRMVELA
                     GLPAGARLLDIGTGTGHTALAFAAGGVEVVGLDMTRAMLEQARGLAGQRGLAFEAVQS
                     YAERLPFADGAFDGIACRYCAHHFDDNPQAIREMGRVLRPEGVLVFVDHVAPEDDEAD
                     TFVNRLDWLRDPSHNREPRLSEYQRWFADAGLTIERVEPFRETMVADEWFARARTAPE
                     REAEARAMLSAASQPLRDTFAISDDPVSFELHMVLIRGARQ"
     gene            complement(3192..4772)
                     /locus_tag="VFV93_08300"
     CDS             complement(3192..4772)
                     /locus_tag="VFV93_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165381.1"
                     /translation="MIQRLLAGVLLLALVAACSGPDNSDPTATPSLPATETTAPAAAT
                     ATIPAPAIATATLPPPSPTATIPQPPTATVTPEPTATLAPTATATPDPQPVGQELGLP
                     DLDAHYRLDISDLDVASGYVRASEVITIQTFRGAAPDRLYLQVVPAEYGFFTLDAASV
                     NGEPVTLETLNGGVTLAHDLPDAPVAPYEIGLEFHLNVGSETTGWGVTTLDGDILRLG
                     NWFPIISTDHGYSATLDPSYTAVASFDVALTLAAGVEFAHTGELGGQKEQPDGRITYG
                     LHAENVRDFALAVSPSYTIDEGQTGTGVALELYTVGVDDGTRQQILAVAADAIDQLSA
                     LIGPYPYATFRIADAGLTLPGGIEFPGMIYINPAYDPLDRLIYHETAHQWLYGIIGNR
                     TLQDGWIDEGGAEFFERGLPTGFSEVPPYPDGGYAYWLDTTYEDLPDDPNRNWYYSIY
                     EQGARFYNDVKATMGDDAFWAAFQDLYTTHAFGIVTAWDMLSNWQQHSPTDLRPLYHE
                     YFRYDWIDALPGAGIGAG"
     gene            complement(4902..4975)
                     /locus_tag="VFV93_08305"
     tRNA            complement(4902..4975)
                     /locus_tag="VFV93_08305"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4941..4943),aa:Cys,seq:gca)
BASE COUNT          940 a         1575 c         1569 g          951 t
ORIGIN      
        1 tggctctgcc gtcgacggct acacgagatg tcttcgcgcc gacattgggc cgtcgaacat
       61 ttcattgccc tttatggtat tgcgggcccc caactcggat gatttgatcc tgtcccgggc
      121 tcggcaatcc aactcacggt gccacccgtc gacgcaacca cggcgacgcc cgacgcgatt
      181 ccgccgaaac catccatcgc ttcgaacgcg atacttgcgc catccagcga ccacgaaggg
      241 ctccaagcac cctgcggcat gtcagtgatg atatgccaat cggatccgtc gctgttcatg
      301 acgataattt gcccccacga gcatgcgatc ttggacccgt ccggtgacca atccggttga
      361 tcgcagcgtg acgtattcgt ggaaagttga cgcaagccgg tcccatcgac tccaatcgtg
      421 tacaacgtcg gtggccctgt accaatatcg gtaaagctaa ccgtcgcgaa cacgagcgac
      481 gtaccatcgg gcgaccagtc tatctccgtt acatgttgat tattcgagaa gtgggtcacc
      541 tgttgggccc cggaaccgtc gttggccagc aggaagatat tgtagttgtc accatggccg
      601 aacgatgaga ccgccaggta cgtctcatca ggtgaccatg cgaagccgcc tccgatgtag
      661 ggatccaaac caactgaacg catgtattcc atacctggat ggggacgttg attcgatcca
      721 tcagcgttca tgagccacag ttcatggggt ttttgaaacg gcgcatgaag atatgcgata
      781 tatcgacctg acggcgacag ctccggcacg aagtcactat ccgaattatt cgacaggttc
      841 cgttcagcag ttccatcggc acgaacggag aagatttcct ggtttcgatt gccatccccg
      901 gtggaaacga gtgttcgata gacgatcacc gagtcatggt cgacttccgg ctgtgcagac
      961 cgccaggcaa ggtaatgccg gccgacgttg ccagcttcca ccttccagcc gtctgggttg
     1021 tctggggtgt acgttaagca gcgtcgctca aaacactgaa gcagtacatc ccgataactg
     1081 ccaccaacct tcactgttgc ccagtatggt tctgcgattg gccgaccggt cgcatacagc
     1141 ggatctgcga agagttgcgc ttcgtggtac gcaccattct cgtagattgt tcccgttgag
     1201 gtcatgaact cccaaaacgg tgtcgcgatt gaatggttgg tcaccggatc gaccgttgcg
     1261 accgtcaccg catacgacgc aagcgctggg tcgtccactg ctgtcgcaga cctgtcgata
     1321 cgctgggtta tccagccccc aaccgggaag gggaggtagg aacgaaggac atcgaatgta
     1381 gcatagactg ggccgatcag atcgtcctgg tcgccggcaa ctggaatctc ggccggctga
     1441 aaagaggaga aactctcatc gccgacctgc atctgtccag tgaccaactc caatacgagc
     1501 agtccgtttg taacgtacca cggcgagttc gggtcgttat ctggatgagt aacctccatc
     1561 cggctcttgt caaaatactg aacgatccgt gtgccatccg gtgattccgc gtatggttca
     1621 gatgttggtg cggtgactgc ttctggcccc cacatccatg tccggacggc tcgtccatcc
     1681 tgcacgggtt gatcagtgcg agcccatgtc cgttgaaacg gtctatcagc gggctgtacc
     1741 ttcgcgaagg tacgttctac actgggaagc gtcagaatca tgctgagaag cgcacaggtc
     1801 acaataagcc atgtggatcg gcacctcatg ccaacacctt ccgcggatgg actcgataag
     1861 aacggctaca ttctatttgt gacatgaaaa atgtgcaacg ctcgcgtcaa cgtcgatcaa
     1921 aactgcacaa atagtcacag ttcatccagc aaatgggcga gcatcacgac gccgtgagcg
     1981 tggccgctgc cgactaatgc ctattgcctc gcacctcgta tcagaaccat gtgcagctcg
     2041 aacgagaccg ggtcgtcgga gatggcgaag gtgtcgcgga ggggctgaga cgcagcggag
     2101 agcatggcgc gggcctcggc ttcgcgctcc ggcgcggtcc gggcgcgggc gaaccactcg
     2161 tcggcgacca tcgtctcgcg gaacggctcg acccgttcga tcgtcaggcc ggcgtcggcg
     2221 aaccagcgct ggtactcgct caggcgcggc tcgcggttgt gcgacggatc gcgcagccag
     2281 tcgagccggt taacgaaggt atcagcctcg tcgtcctcgg gcgcgacgtg atcgacgaag
     2341 accagcaccc cttcgggccg caggacacgc cccatctcgc ggatcgcctg tgggttgtcg
     2401 tcgaagtggt gcgcgcagta gcggcaggcg atgccgtcga acgcgccgtc cgcgaacggc
     2461 agccgctcgg cgtaggactg gaccgcttcg aatgccagcc cgcgttgccc ggccagcccg
     2521 cgcgcctgtt cgagcatcgc gcgggtcatg tccagcccga cgacctcgac gccaccggct
     2581 gcgaatgcca gtgccgtgtg accggttccg gtaccgatat ccagcagccg cgcgccggcc
     2641 ggcagcccgg ccagctcaac catccgtggc aggtcctgcc cctgggcgtg gatcgcgctg
     2701 gtgacatagg cgctggcgat cggtgtgtac tgctcgtctg agcggcggac atcccggtcc
     2761 gcccgcgtct ggtcaaccac ggttgttccc ttccctcatt gccgcaacgc caggagtcta
     2821 gcagccaatc agggagaagg tgcagtggca ggtagtcatc agtcaccccg cagagtcgcc
     2881 aaaagtcttg gtccggggcc gcgcaagtcc cggccttacg tcaacaaccc acactcacgg
     2941 gtgatacccg gccggagcgc cgcaacctat cattcctatc agaacacgac ggacgagaca
     3001 gccgaacctc cctgcagcgg cagccatcgc ccggcctgtt cacagcaatt attaattctc
     3061 ctccctcccc ttcctccctc gggcaggcgg ccggttccca ctagcggacc ggccgcttgc
     3121 ttttttcgtc gggagtcggg gttcgggatt cggggccgga aggccgagcg aatcttcagg
     3181 aacatcgaac gtcacccagc cccgattccc gcccccggca acgcatcaat ccagtcgtag
     3241 cggaaatact cgtggtagag ggggcgcagg tcggtcgggc tgtgctgttg ccagttggag
     3301 agcatgtccc aggcggtgac gatcccgaag gcatgggtgg tgtagaggtc ctggaatgcc
     3361 gcccagaagg cgtcgtcgcc catcgtggct ttgacatcgt tgtagaagcg ggcaccctgc
     3421 tcgtagatcg agtagtacca gttgcggttg gggtcgtccg gcaggtcctc gtaggtcgtg
     3481 tcgagccagt aggcgtagcc gccgtccggg tagggtggca cctcactgaa gccggtcggc
     3541 aggccgcgct cgaagaactc cgccccgccc tcgtcgatcc agccgtcctg gagcgtccgg
     3601 ttgccgatga tcccatagag ccactggtgg gccgtctcgt ggtagatgag ccggtcgagc
     3661 gggtcgtagg ccgggttgat gtagatcatg ccggggaact cgatcccgcc cggcagggtc
     3721 aaccccgcgt cggcgatccg gaaggtcgcg tacgggtagg gaccgatcag cgcagagagc
     3781 tggtcgatcg cgtcggccgc gacggccagg atctgctgcc gggtgccgtc atcgacgcca
     3841 acggtgtaca gctccagcgc gacgccggtt ccagtctggc cctcatcgat cgtgtaggag
     3901 ggcgagacgg ccagcgcgaa atcgcggacg ttctcggcgt ggaggccgta ggtgatgcgg
     3961 ccgtcgggct gctccttctg gccgcccagc tcgccggtat gggcgaactc aacgccggca
     4021 gccagcgtca gcgcgacatc gaacgaggcg acggccgtgt aggacggatc gagcgtcgcg
     4081 ctgtagccgt ggtcggtcga gatgatcgga aaccagtttc ccagccgcag aatatcgcca
     4141 tccagcgtcg tcacacccca gccggtcgtc tcgctgccga cattgaggtg aaactccaac
     4201 ccgatctcgt acggcgcaac cggcgcgtcc ggcagatcgt gcgccagcgt gacgccgcca
     4261 ttgagcgtct ccagcgtgac cggctcaccg ttgacgctgg ccgcatcgag cgtgaagaag
     4321 ccgtactcgg ccgggacgac ctgcaggtag agccggtccg gcgccgcacc ccgaaacgtc
     4381 tggatcgtga tcacctcgct ggcccgaacg tagccgctgg cgacatccag gtcgctgata
     4441 tcgagccggt agtgcgcgtc cagatcgggc agcccaagct cctgcccgac cggctgcggg
     4501 tcgggcgttg ccgtcgcggt tggcgccagc gtggcagtcg gctccggcgt gacggtcgcg
     4561 gttggcggct ggggtatcgt cgcggtcggt gagggcggcg gcagcgttgc cgtggcgatc
     4621 gcgggggcgg ggattgtcgc cgtcgcggcg gctggtgcgg tcgtctccgt cgccggcagc
     4681 gatggtgtcg cggtcgggtc gctgttgtcc ggcccgctac aggccgcgac cagcgccagc
     4741 agcagcaccc cggcgagcag acgctgaatc atcgcaggca attcccctca gttgacggtt
     4801 gaactggcag catacccacc acaactccgc gcgatgccac acgttaccaa caaaaaacac
     4861 ccgccttggt gcgggtgtct cgcgctcatg actgacggtg ctggaggcgt cgggcggatt
     4921 cgaaccgccg atcagagttt tgcagactcc tgccttgccg cttggctacg acgccgaaag
     4981 gcttgtggac tgggattcta ccagatgcgg ttcctcagag tcacgctgcc tgtcc
//
LOCUS       DASUGJ010000375         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_613106, whole genome shotgun sequence.
ACCESSION   DASUGJ010000375 DASUGJ010000000
VERSION     DASUGJ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613106"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..451
                     /locus_tag="VFV93_08310"
     CDS             71..451
                     /locus_tag="VFV93_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5165382.1"
                     /translation="MLIAREADRDVDLWRDGVETRVYASATAGSRQLVVFEQVCAPGF
                     GAPAHLHAVEEVLRVIEGEAEVSVGDERATLSPGDAVTIPAGIVHGFTNSGEAPLRVL
                     AILASPTFEAHYVDPPRDSRRWLP"
     gene            complement(464..754)
                     /locus_tag="VFV93_08315"
     CDS             complement(464..754)
                     /locus_tag="VFV93_08315"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5165383.1"
                     /translation="MKTIEMPTATDEQLVEMLRAVANPVRYRILQILAERGECQCGPL
                     GDLLPIAPSTLSQHLKVLREAGLISGTVDGPTICYCREEQGIAWLKDHIASL"
     gene            complement(876..1709)
                     /gene="arsM"
                     /locus_tag="VFV93_08320"
     CDS             complement(876..1709)
                     /gene="arsM"
                     /locus_tag="VFV93_08320"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX5165384.1"
                     /translation="MTQQQKPAASAIREVVRERYASAVTAVLNRESASCCGGSTTEAS
                     CCGGGSVVSPIVIGNYTPGETASLPEEAVLASFGCGNPVALAKLEPGQTVLDLGSGGG
                     IDVLLSAKRVGPTGFVYGLDMTDEMLELAERNKEAAGIDNVRFLKGEIETIPLPDAAV
                     DVIISNCVINLAADKDVVLADAFRVLRPGGWFAVSDVVVQGELTDELRSSMDAWAGCV
                     AGALEEQDYLRRLTAAGFVDASIEVTSTYDLADIGCCADAAYTTNGSVVSAFIRARKP
                     E"
     gene            complement(1796..>2413)
                     /locus_tag="VFV93_08325"
     CDS             complement(1796..>2413)
                     /locus_tag="VFV93_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX5165385.1"
                     /translation="LFASVSPRYAHLNLGIERADSVGADGHKWLNVPYDCGFAFVRDA
                     GALRAAFNASGAYIAPAPDLGPDPMAHLPEMSRRFRGLAAWAALRAYGRSGYREMVER
                     CADNAASFANWVEQTDGLELMAPAPLNIVCFRYAPSGLDDGATDAFNRDAIAAIQRDG
                     RAFVTGTVWNGRAAIRAAFDNWATTAADVEILQAAVRDQRPGERR"
BASE COUNT          439 a          803 c          763 g          408 t
ORIGIN      
        1 tcacaaccgc tgccgcgtga gcggggcgtc gaactggaca gcttcgacaa cggactggag
       61 acgatcgagc atgctcatcg cacgtgaggc agaccgggac gtcgatctct ggcgcgacgg
      121 cgttgaaacg cgggtctacg cgtcagccac ggcaggtagc cggcagctgg tcgtcttcga
      181 gcaggtctgc gcgccaggct tcggcgcgcc cgcccacctg catgccgtcg aggaggtgtt
      241 gcgggtgatc gagggcgagg ctgaggtcag cgtcggcgac gaacgggcga cgctcagccc
      301 tggcgatgcc gtcaccattc cagcagggat cgttcacggc ttcaccaaca gcggggaggc
      361 cccgttgcgc gtgctggcga tcctcgcctc gccaaccttc gaggcccact acgtcgatcc
      421 accgcgcgac tcgcgccgct ggctaccctg accactcagg atgtcaaagg ctcgcgatat
      481 ggtccttgag ccaggcgatg ccctgctcct cgcggcagta gcagatggtt ggcccgtcaa
      541 cggtgccgga aatcagtccc gcctcgcgta gcaccttgag gtgctgggag agcgtcgagg
      601 gcgcgatcgg gagtagatcc ccaagcgggc cgcactgaca ctctccgcgc tcggccagaa
      661 tctgcaggat gcggtatcgg accgggttgg cgaccgcgcg cagcatctcc actagttgct
      721 cgtcggtcgc cgtcggcatc tcaatcgtct tcatcagtca gtcagccagt cagttcgtca
      781 accggttagc ccgtcaatcg ggttgcgtcc gtcgcgtccg atcatagcac ctcagtcatc
      841 gtcgactctc gatggatgtc tgtcgcgccg gtgagctact ccggcttacg agcgcgaatg
      901 aaggcactga caacactccc gttcgtggtg taggcagcgt ccgcgcagca gccgatgtca
      961 gccaggtcgt aggtgctggt cacctcaatg ctggcgtcaa caaagcccgc ggcggtgaga
     1021 cggcgcaggt aatcctgctc ttcaagcgca cccgcgacgc aaccggccca ggcgtccatg
     1081 ctggagcgca actcatccgt cagctcaccc tggacgacaa cgtccgagac ggcaaaccaa
     1141 ccgcccggtc gcaggacgcg gaatgcatca gccaggacga catccttgtc ggcggcaagg
     1201 ttgatgacgc agttcgagat gatcacgtca accgcggcgt caggcagcgg gattgtctcg
     1261 atttcgccct tgaggaaacg gacgttgtcg atcccggctg cctctttgtt acgctcggcc
     1321 agttccagca tctcgtctgt catgtcgagt ccgtagacga agcccgttgg gccgacccgc
     1381 ttggccgaca gcaacacgtc gatcccgccg cccgatccaa gatcgagcac cgtctggccc
     1441 ggctccagct tcgccagcgc gactggattg ccacaaccaa acgaggccag caccgcctcc
     1501 tcggggaggc ttgccgtttc gcccggggtg taattaccga tcacgattgg cgaaacgacg
     1561 ctgccgccac cgcagcaact cgcttcagtc gtcgacccgc cacaacatga tgccgactct
     1621 cggttcagga cagctgtgac ggcgctcgca taccgctcgc gaactacctc gcgaattgcg
     1681 ctggcggcgg gtttctgctg ctgtgtcatc ggttagctcc ctcgaaaaaa gatgatgctt
     1741 catttcgtaa aaatacgacc agagcgaccg gctgtcaaga cccgattcag tacagtcatc
     1801 gtcgctcgcc cggtcgttga tcgcggacgg cggcttgaag gatctcgaca tcggcggcag
     1861 ttgtcgccca gttgtcgaag gcagcccgaa tcgcagcccg gccattccag accgtgccgg
     1921 tcacgaacgc gcggccatcg cgctggatcg cggcgatcgc atcccggttg aacgcatcgg
     1981 tcgcgccgtc atcgagcccc gacggggcgt aacggaagca gacgatgttg agcggggccg
     2041 gcgccatcag ctccaggccg tccgtctgct cgacccagtt cgcgaacgag gccgcgttgt
     2101 cggcacagcg ctcgaccatc tcccggtagc cggaccggcc gtaggcacgc agcgcggccc
     2161 aggctgccag cccgcgaaag cgccgggaca tctccggcag atgcgccatc gggtcagggc
     2221 cgaggtcggg cgccggcgca atataggcgc cggacgcgtt gaaggcggcc cgcaacgcac
     2281 cggcatcgcg gacgaaggcg aagccgcagt cgtacggcac attcagccac ttgtgaccgt
     2341 cggcgccgac ggaatctgcc cgctcgatgc ccaggttcag gtgcgcatag cggggcgaga
     2401 cactggcgaa cag
//
LOCUS       DASUGJ010000376         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1587840, whole genome shotgun sequence.
ACCESSION   DASUGJ010000376 DASUGJ010000000
VERSION     DASUGJ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587840"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VFV93_08330"
     CDS             <1..956
                     /locus_tag="VFV93_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX5165386.1"
                     /translation="LHRASIMLPIHDNDHPGIWLGAVMGAAQREGRDKLTLIASPGWE
                     PFGDWLEQLIAESTGKDGTGIVPVTGESLAGPEAYGDDRLFVYFRGAEQTAACDKAVA
                     ALRKAGHPVVQIEMDSPDALGREFVRWEIATAVAGGLMGINPFDEANVQEAKDATNAV
                     LADPGLSVPAATAADDAAAKLMESTKPHGYIAILAYVDRTPQTEAALDDLRDALRKRS
                     GLATTLGYGPRYLHSTGQLHKGGTENGTFLQIVATPSSDIEIPGVKYGFKTLFAAQSA
                     GDAITLDRHGLPLVRVEVGDDATGGIGEITSAVGAKTATAR"
     gene            1003..1899
                     /gene="gnd"
                     /locus_tag="VFV93_08335"
     CDS             1003..1899
                     /gene="gnd"
                     /locus_tag="VFV93_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX5165387.1"
                     /translation="MDIAIIGLGRMGANMVRRLLQDGHRVVAHNRSRGPIDEMAAEGA
                     VAAYELEDVIKELKAPRAVWLMVPAGDPVDQQIEALVPLLDKGDIIIDGGNSRYTDTI
                     ARHDRLKQQGIHYLDAGTSGGVWGLQVGYCLMVGGPDEAVKVVEPAFKSLAPEGGYLH
                     IGPSGSGHFVKMIHNGIEYGMMQAYAEGFEILEKSRYDVDLRAVADLWMHGSVVRSWL
                     LELAERAFAEDPHLDSIKGYVNDSGEGRWTVFESIDLDVAAPSIYLALAMRFLSRQED
                     TFGNKVLAALRNQFGGHAVVRE"
     gene            2023..>3480
                     /gene="zwf"
                     /locus_tag="VFV93_08340"
     CDS             2023..>3480
                     /gene="zwf"
                     /locus_tag="VFV93_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5165388.1"
                     /translation="MTVAPRTVTPAEAEELEAGPNALREGLLIERTPEPFAMVIFGAS
                     GDLTRRKLIPALYNLALERLLPAGFSIVGFARHDMSTHSFRAKMREAVDEFSRRRPVD
                     DAVWSSFAEGLSYVRGDFTDASAYDQLGKHLSELDEKRGTLGNRLHYLATPPDYYDDI
                     IEQLGAHDLNHGPENTWQRIIVEKPFGHDLESARKLNVTVNRVFDERDVYRIDHYLGK
                     ETVQNIIAFRFANGIFEPLWNRQYVDHVQITVAESLGVEGRGGYYDQTGALRDMVQNH
                     LMQLLTLVAMEPPVELKADPVRDEKVKVLRAVRPIKPDLVRRFTVRAQYREGFEAGEP
                     VPGYREEEKIDPESRTETYVALKLFVDNWRWAGVPFYLRTGKRLPRRVSEIAIQFKRA
                     PHLLFSGGSVERMEPNVLTLDIQPDEGTSLKFAVKVPGPMMNLRSVHMGFQYGTSFNV
                     QSPDAYERLLLDALLGDPTLFTRRDEVETAWTIVND"
BASE COUNT          640 a         1088 c         1185 g          567 t
ORIGIN      
        1 tgctgcaccg ggcgtcgatc atgttgccga ttcacgacaa cgaccacccc ggcatctggc
       61 tgggggcggt gatgggcgcc gcccagcgcg agggtcgcga caagctgacg ctgatcgcca
      121 gccccggttg ggagccgttc ggagactggc tggagcagtt gatcgccgag agtaccggca
      181 aggacggtac cgggatcgtc ccggtcaccg gcgagtcgct ggccggcccg gaggcgtacg
      241 gcgacgaccg gctgttcgtc tacttccgag gggcggagca gacggccgcg tgcgacaagg
      301 ccgtggccgc gttgcgaaag gcggggcacc cggtcgtcca gatcgagatg gactcacccg
      361 acgcgctcgg ccgcgagttc gtgcgctggg aaatcgcgac ggcggtcgcc ggtggactga
      421 tggggatcaa cccgttcgat gaagcgaatg ttcaggaagc gaaggacgcg accaacgccg
      481 tcctggctga tcccggcctg agcgtcccgg ccgcgacggc agccgacgac gcggcggcga
      541 agctgatgga atcgacgaag ccgcatggct acatcgcgat tctggcctat gtcgaccgga
      601 caccgcagac cgaagccgcg ctcgacgacc tccgcgacgc gctgcgcaag cgatccggcc
      661 tggcgacgac gctcggctac ggcccgcgct acctgcactc aaccggccag cttcacaagg
      721 gcgggacgga gaacggcacc ttccttcaga ttgtcgcaac cccatcgtcc gatatcgaga
      781 tcccgggtgt gaagtacggg ttcaagacgc tgttcgcggc gcagtcggcc ggtgatgcga
      841 tcacgctcga ccggcatggc ctgccactgg tgcgggttga ggtcggcgac gacgcgaccg
      901 gcggcatcgg agagataacg agcgcggttg gggcgaagac ggcgaccgct cgctagccac
      961 gacctgttga ttgccgacgg ctgacaacgt gaaggaggct caatggacat cgcgatcatc
     1021 gggttggggc ggatgggcgc gaacatggtg cgccggctgc tacaggacgg acaccgcgtc
     1081 gtcgcgcata accggagccg cggtccgatc gatgagatgg cagccgaagg cgctgttgcg
     1141 gcctacgagc ttgaagatgt catcaaggag ctcaaggcgc ccagggcagt ctggctgatg
     1201 gtgccggctg gagatccggt cgaccagcag atcgaagctc tcgtgccgct actcgacaag
     1261 ggcgacatca tcatcgacgg cggcaattcg cgctacaccg acacgatcgc gcgccacgac
     1321 cgcctcaagc agcagggcat ccactacctc gacgccggga catcgggtgg tgtctggggc
     1381 ttgcaggtcg gctactgcct gatggtcggc gggccggatg aggccgtcaa ggtcgtcgag
     1441 ccggccttca agtcgctcgc gccggagggc ggctacctgc acatcgggcc gtccggatcg
     1501 ggccacttcg tcaagatgat ccacaacggg atcgagtacg gcatgatgca ggcctacgcc
     1561 gagggcttcg agattctgga gaaatcgcgc tacgacgtcg atctgcgagc cgtcgccgat
     1621 ctctggatgc acggctcggt cgtccgttcg tggctgctgg agctggccga gcgcgccttc
     1681 gccgaggacc cgcacctgga ctcgatcaag ggctacgtca acgactcggg cgagggtcgc
     1741 tggaccgtct tcgagtcgat cgacctggat gtcgcggcgc catcgatcta tctggcgctg
     1801 gcgatgcgct tcctttcgcg ccaggaggac accttcggca acaaagtgct ggcagccctg
     1861 cgcaaccagt tcggcggcca cgccgtcgta cgggaatagg cgttgtgagg cgataggtga
     1921 taggtggtag gtgttggtta gtaccagagg aactcggcac gttgattccc accgaccagc
     1981 gcctgacaac tatcacctcg caacgcctcg attggatggt agatgacagt agcaccgcgg
     2041 accgtcaccc cggcggaagc tgaagaactg gaggccggcc cgaacgcgct gcgcgaaggg
     2101 ttactgatcg agcgaacgcc agagccgttc gcgatggtga tcttcggcgc atcgggtgac
     2161 ctgacacgcc ggaagctgat tcccgccctc tacaacctgg cgctggagcg gctgcttcca
     2221 gccggattca gtatcgtcgg gttcgcccgc cacgacatgt cgacccattc gttccgggcc
     2281 aagatgcgcg aggccgtcga cgagttctcg cgtcgccgtc ccgtcgacga cgcggtctgg
     2341 tcgtcgtttg ccgaggggct gagctacgtg cgcggcgatt tcaccgacgc ctcggcgtat
     2401 gaccagcttg gcaagcacct gtcggaactc gatgagaagc gcggaacgct cgggaaccga
     2461 ctgcactatc tggcgacgcc acccgactac tacgacgaca tcatcgagca actcggggca
     2521 cacgacctaa accacggccc ggagaacacc tggcagcgca tcatcgtcga gaagccattc
     2581 gggcatgatc tggaatcggc gcggaagctg aacgtcaccg tcaaccgggt cttcgacgag
     2641 cgcgacgtct accgcatcga ccactacctg ggcaaggaga cggtccagaa catcatcgcc
     2701 ttccgcttcg ccaacgggat cttcgagccg ctttggaacc gccagtacgt cgatcatgtc
     2761 cagatcaccg tcgccgagtc gctgggcgtc gaagggcgcg gcggctacta cgaccagacc
     2821 ggtgcgctgc gtgacatggt ccagaaccac ctgatgcaac tgctgacgct ggttgcgatg
     2881 gagccgccgg tcgagctgaa ggccgatccg gttcgcgacg agaaggtcaa ggtcctgcgg
     2941 gccgtccgac cgatcaagcc cgacctggtg cggcgcttca ccgttcgcgc gcagtaccgg
     3001 gagggattcg aggccggcga gccggtaccg ggctatcgcg aagaggagaa gatcgatcct
     3061 gaatcgcgga ccgagacgta tgtcgcgctg aagctgttcg ttgacaactg gcgttgggcg
     3121 ggggtgccgt tctacctgcg gaccggcaag cggctgcccc gacgggtcag cgagatcgcg
     3181 atccagttca agcgggcgcc gcacctgctc ttcagcggcg gctcggtcga gcggatggag
     3241 ccgaacgtgc tgacgctcga catccagccg gatgagggca cgtcgctcaa gttcgccgtc
     3301 aaggtgccgg ggccgatgat gaacctgcgg tcggtccaca tgggctttca gtacgggaca
     3361 tcgttcaacg ttcagtcgcc ggacgcctac gagcggttgc tgctggacgc gctgctgggc
     3421 gatccgacgc tgttcacccg gcgcgacgag gttgagacgg cctggacgat cgtcaacgac
//
LOCUS       DASUGJ010000377         6245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1031670, whole genome shotgun sequence.
ACCESSION   DASUGJ010000377 DASUGJ010000000
VERSION     DASUGJ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031670"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(622..2103)
                     /gene="tcuA"
                     /locus_tag="VFV93_08345"
     CDS             complement(622..2103)
                     /gene="tcuA"
                     /locus_tag="VFV93_08345"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX5165389.1"
                     /translation="MHEHEWDVVVVGAGNAALSAALSAREQGAEVLVLESAPVEACGG
                     NSRFTGGMVRIVYNSVDDLLQVVPDLDEDERVNTDFGAYTREQFFDDMARVTQYRADA
                     DLVRIMVEQSLPTMVWLREQGVRFVPAYGHQAYKVDGKFKFWGGLTVQASGGGSGLVA
                     ALIAAAEKRGIPIRYATRAVGLVETDGQVEGVVVRHAGQTQTIRANAIVLACGGFESN
                     AEWRTRYLGPGWDLAKVRGTRFNMGDGIRMALDIGAMPHGNWSGGHAVAWDRNAPDFG
                     DLAVGDAFQKHSYPLGIIVNARGERFVDEGADFRNYTYAKYGHEVLRQPGQFAWQVFD
                     SKVIPMLRDEYRIRQVTRVRANTLEELAARLDDVDPRGFLEMIEAFNAAVCTDVPFNP
                     AVLDGRGTRGLAIPKSNWANRLDTPPYEAYQVTCGLTFTFGGLKIDDQARILDVEDRP
                     IPGLYAAGELVGGLFYFNYPGGTGLTSGAVFGRIAGQSAATLS"
     gene            complement(2133..2915)
                     /locus_tag="VFV93_08350"
     CDS             complement(2133..2915)
                     /locus_tag="VFV93_08350"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5165390.1"
                     /translation="MRRFPGPATPGKTDQPNCSVVPEATAHPLLLCAQHRGSVWCGQW
                     IGTSRSIQQALFVYLRDCVEHLGSNIFWCSQLKCVPLTDSAEQELVCLRPGFPKCGWR
                     GPVIPVQRLDRILRRRGSRSRQEPPARGAHAWDRAADEAPCLVEGQLIGGAVQGISET
                     LWEQVTYNEEGQLLSSTLMDYAVPRAEWLPTFELECTTTPTPRNVLGAKGVGEAGTVH
                     APPAVANAAMDALRPFEVEPLDLPLTAEKLWRVINYGGHGIR"
     gene            complement(2875..3528)
                     /locus_tag="VFV93_08355"
     CDS             complement(2875..3528)
                     /locus_tag="VFV93_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5165391.1"
                     /translation="MAETDPARLSMPLGEAIYTQRSIRRLRPDPIPIDDIHTIIEAAS
                     KAPNGGNAQPARYLVLTDRDVIRRFGALYREAWWAKRRDEGRPWTRREEIPTEERVSR
                     GAAKLADEMADAPCIVLALTERKGQASSVLPGVQNLMLAARALGIGSVPTTLHPQVMD
                     RVYELLRIPASAELHLCIPLGYPRGGFGPTRRRPTAETTYLNHWGAPVPWTGDSGED"
     gene            complement(3564..4814)
                     /locus_tag="VFV93_08360"
     CDS             complement(3564..4814)
                     /locus_tag="VFV93_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5165392.1"
                     /translation="MADMTAAQEPTDEIVAAAESIFPGRSSGSYAVPPWMQNVVVASG
                     QGSRFFTAAGDGYLDYILGSGPLILGHAHPRVVAAVREQASRGSTFYALNEPIIRLGQ
                     MIVDAVPCAEQVMFASTGAEATYLALRLARAATGRDLILRFAGSYHGHHDYVSTQSAG
                     VPQAARETVLTVTFNDLASVEKQLAAHPGAVAAVIVEPLHRVVRPAPGFLDGLAQVTR
                     AAGALLIFDEVVTGFRLAWGGGQERYGVTPDLATYGKIIGGGYPLSAICGRREIMELA
                     SPRRNGQPDYVFISGTLSGNPIGATAGLVTLTELRQPGAYERLDQVGERLRRGFNDVA
                     EEFDLPLEMRGEGAVSGVVATDGGAANGSALLPALGSALFQQGIASNLGKVYVSLAHS
                     DADIDQTIRAYSVALSQVRRDMPQ"
     gene            complement(4940..5905)
                     /locus_tag="VFV93_08365"
     CDS             complement(4940..5905)
                     /locus_tag="VFV93_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165393.1"
                     /translation="MFCSSTVGSITRRSLRLAWWSGHSHGRYAGSTWYADEFALDLME
                     HCVSHINGDSPGCRWATVYDNVMRVEAAADLVSQIVKDVTGQESTGLRPLRAGDNSFN
                     NIGIPLYFMLSSTMPEDLRLEKSYYGVAGCGGNIAWHTVDDTLEIADRDNLLRDIKVY
                     AVAALRSLNAPVHPFDYRAEVDDIRSVVERYQAAANGKVDFSQALEELNRLRDVLDRF
                     YRNIEERAPSDPVAASAANAVQRTLARTLLFVSYSRAGRFRQDPASQTPPLPDLAPAR
                     QLAELEAGSDMANAIGVHLKRGQNRLVWACRTSTQQVEAYLSGQS"
     gene            5874..6047
                     /locus_tag="VFV93_08370"
     CDS             5874..6047
                     /locus_tag="VFV93_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165394.1"
                     /translation="MIEPTVDEQNILEETLFDEAEFLKTFGLDRFLLDLRGQQLKIKD
                     MFHRSHRSWRRLC"
BASE COUNT         1098 a         2076 c         1997 g         1074 t
ORIGIN      
        1 agcgcgttga cgaacagccc gccgctcatg ccgattcgac ggtggatccg ccagacggcc
       61 agatcgatcc cgaccgattc aagtgcgtcg cgccacgcga gcacatgctg atacacgctg
      121 tcgaccagcg tgccatcgag atcgaagagg aaggcaggac cattccgcgt cattctgact
      181 ccatgttggc cggagacgca cggatcgacg aagacacggt gacgttgcca ctcagggacc
      241 tgcgaggatc ctcctccgcg agcacagccg ggcgacggag gcgctgggca tcgggttctg
      301 accggtacca agttgccgcc cgcacggcgt aatcggccct cggtgcggcc ggcacgatcg
      361 ttgcgccgag ctgcgctagc tgcgactcca gcaagaatag gaccatctcc tgcggttcca
      421 tcacggtctc cccggcattg gtgagcggga cgagcgttcg tctggcgatg gccagtgtcc
      481 catccacacc acctgggtcg tcacgacctg accgtcgcgt tgtcgtcgcg agagacgacg
      541 gtgacgccat cgccgacaat cgagatctgg tattccccgg tctcggccgc gaggccgatc
      601 cgcacttgaa gcgaccatcc tttaagacag cgttgcggca gactggccag cgatgcggcc
      661 gaagacggcg ccactcgtca gtccggtccc gccggggtag ttgaagtaga acagcccgcc
      721 gaccagttcg ccggctgcgt acaagcccgg gatcggccga tcctcgacgt ccaggatccg
      781 tgcctggtca tcgatcttca atccgccgaa ggtgaacgtc agcccacagg tgacctgata
      841 cgcctcatac ggcggcgtgt cgagccggtt ggcccagttc gacttcggaa tcgcgagccc
      901 ccgcgtcccc cggccgtcga gcaccgccgg attgaatgga acgtcggtgc agacggcggc
      961 gttgaacgcc tcgatcatct ccaggaagcc gcgcggatca acatcgtcga gtcgcgcggc
     1021 cagttcctca agagtgttcg ctcgcactct ggtcacctga cggatccgat actcgtcgcg
     1081 cagcatcggg atgaccttgc tgtcgaagac ctgccaggca aactgccccg gttggcgcag
     1141 cacctcgtgc ccatacttgg cataggtgta gttgcgaaag tccgccccct catcgacgaa
     1201 gcgctcgccg cgagcgttga cgatgatacc gagcgggtag gagtgcttct ggaaggcatc
     1261 gccgaccgcg aggtcgccga agtcgggtgc gttgcggtcc caggcgacgg catggccgcc
     1321 ggaccagttg ccgtgcggca tcgcaccgat gtcgagcgcc atccggatcc cgtcgcccat
     1381 gttgaagcgg gtaccgcgca ccttggcgag atcccagccg gggccgaggt agcgggtccg
     1441 ccactcggcg ttcgattcaa acccgccaca ggccagcacg atggcgttgg cccggatcgt
     1501 ttgtgtctgc cccgcatggc gaacgacgac cccttcgacc tgaccgtcgg tctcgacgag
     1561 gccgacggca cgggtcgcat accggatcgg aatgccacgc ttctcggcgg ccgcgatcag
     1621 ggccgccacc agcccggacc caccaccgga ggcctgaacc gtgagcccgc cccagaactt
     1681 gaacttcccg tccaccttat aggcctggtg accataggcg ggcacgaagc gcaccccctg
     1741 ctcgcgcagc cagaccatcg tcggcaggct ctgctcgacc atgatccgta cgaggtcggc
     1801 atcggcgcgg tactgcgtca cccgcgccat gtcgtcgaag aattgctcgc gggtgtaggc
     1861 accgaagtcg gtgttgacgc gctcatcttc gtcgagatca gggacgacct ggagcagatc
     1921 atcgacactg ttgtagacga tgcgcaccat accgccggtg aagcggctgt taccgccgca
     1981 cgcctcgacc ggtgcgctct cgaggaccag gacctcggca ccctgttcac gagccgacag
     2041 cgcagccgac agggcagcgt tcccagctcc aacgacgacg acatcccact cgtgttcgtg
     2101 catggctcta tgctcctcgc ctcgacgttg cgttagcgga ttccgtgccc accatagttg
     2161 atcacgcgcc agagcttttc ggctgtcagc ggcaggtcga gcggttcaac ctcgaacggt
     2221 cgcagcgcat ccatggcagc gttggcaacg gctggtggag cgtggaccgt tccggcctcg
     2281 ccaacgccct tggcaccgag cacgttgcga ggtgttgggg tcgtcgtgca ctccaactca
     2341 aacgtcggca accattccgc acgcgggaca gcgtagtcca tgagcgtgct tgagagcaat
     2401 tgcccctctt cgttgtaggt gacttgctcc cagagggtct cgctgatccc ctggacggcg
     2461 ccgccaatca actgtccctc aaccaggcag ggtgcttcat ctgcggcacg atcccaagcg
     2521 tgtgcaccgc gcgctggagg ttcttgccga gacctactcc cgcgacgtct cagaatccgg
     2581 tccaggcgtt gaactgggat gaccggacca cgccacccac acttcgggaa gccagggcga
     2641 agacagacaa gctcctgctc ggcggaatcg gtcagtggga cacacttcag ttgggaacac
     2701 cagaagatgt tcgagcccag gtgctcgacg caatcgcgca ggtagacgaa aagggcttgt
     2761 tgaatgctgc gggatgtacc tatccactga ccacaccaga cggaacctct atgctgcgcg
     2821 caaagcagta gaggatgcgc ggtagcctcc ggtacgaccg agcagttggg ctggtcagtc
     2881 ttccccggag tcgccggtcc agggaaccgg cgcaccccaa tggttgaggt acgtcgtttc
     2941 ggcggtgggg cgacgccggg tcgggccgaa cccgccgcgc gggtagccaa gtggaatgca
     3001 cagatggagc tcggccgatg ccgggatccg cagcaactcg tagacccgat ccatcacctg
     3061 gggatgcagc gtcgtcggca ccgagccgat ccccaacgcc cgggcggcga gcatcagatt
     3121 ctggacgccg gggagcaccg agctggcctg tcccttccgc tctgtcagcg ccagcacgat
     3181 gcacggcgcg tcggccatct cgtccgccaa tttagcggcc ccacgtgaga cgcgctcctc
     3241 ggtcgggatc tcctcccgcc gcgtccacgg tcgcccctcg tcgcgccgct tggcccacca
     3301 cgcctcacgg tagagcgcgc cgaaccggcg aatcacgtcc cggtcggtga ggacgagata
     3361 gcgggcgggc tgagcgttgc caccgttcgg cgccttgctc gccgcctcga tgatcgtgtg
     3421 gatatcgtcg atcgggatcg ggtccggccg cagccggcgg atcgaccgct gcgtatagat
     3481 cgcctcaccc agcggcatcg agagccgtgc cggatctgtc tcagccatcg ttgacctccc
     3541 cacgcgtcct gcgccggaac tgcttattgc ggcatgtcgc gtcgcacttg cgacaatgcc
     3601 acactatagg cgcggatcgt ctgatcgatg tcggcatcgc tgtgcgcaag tgagacgtag
     3661 accttgccca gattactggc gatcccctgc tggaagagtg cgctcccaag tgccgggagg
     3721 agtgccgatc cgttcgccgc gccaccgtcg gtcgcgacga cgccgctgac cgcaccttca
     3781 ccgcgcatct ccagtgggag gtcgaactct tcggccacgt cgttgaagcc gcgccgcagc
     3841 cgctcaccga cctggtcgag ccgctcgtag gcgccgggct ggcgcaactc ggtcagtgtc
     3901 acgagtccgg ccgtcgcgcc gatcggattg ccgctgagcg tcccgctgat gaagacgtag
     3961 tcaggctggc cgttgcgccg cgggctggcg agttccatga tctcgcgccg gccgcagata
     4021 gctgacagtg ggtagccccc gccaatgatc ttgccgtagg tcgccaggtc cggcgtcacg
     4081 ccgtaacgct cctggccgcc gccccaggct agccggaagc cggtgacgac ctcatcgaag
     4141 atgagcagtg ccccagccgc acgcgtgacc tgtgcgagac catcgaggaa accgggcgct
     4201 ggacggacga cacgatgcag cggttcgacg atgacggcgg cgaccgcacc aggatgcgcc
     4261 gccaactgct tctcgacaga cgccaggtcg ttgaacgtca ccgtcagcac ggtctcgcgg
     4321 gctgcctgcg gaacaccggc ggactgggta ctgacgtagt cgtgatggcc gtggtagctg
     4381 ccggcaaagc gcaggatcag atcacgaccg gttgcggcac gagcgaggcg cagcgccagg
     4441 taggtcgcct cggcaccggt gctggcaaac atgacctgct cggcgcatgg aactgcatca
     4501 acgatcatct gccccaggcg gatgatcggc tcgttgagcg cgtagaaggt cgagccgcga
     4561 ctggcctgct cccggacggc agcgacgacc cgcgggtgcg cgtggccgag gatcaacggg
     4621 cctgagccca ggatgtaatc cagatagccg tcacccgccg ccgtgaagaa gcggctgccc
     4681 tggccggagg cgacgaccac gttctgcatc cacggcggca cggcatacga gccgctactc
     4741 cggccgggaa agatcgactc ggcagcggcg acgatctcgt cggtgggctc ttgtgcagcg
     4801 gtcatgtcag ccatcagcgt ccctcctggt ctttcggtct gatcctcaac tccagtcgcg
     4861 ctcgatacca ccagtgccat cggtcgaatc gctacgccag tggtgtcgag cgacgattct
     4921 gtgatctggg atccgtttct caggattgcc cactcagata cgcttcgacc tgctgcgtcg
     4981 acgttcgaca cgcccacacc agacggttct ggccgcgttt cagatgcact ccaatcgcgt
     5041 tggccatgtc gctgcctgct tccagctctg ccagctggcg tgcgggcgcg aggtccggca
     5101 gcggcggggt ctggctggcc ggatcctggc gaaaacgacc ggcgcgactg tacgacacga
     5161 acagcagcgt gcgcgccagt gttcgctgga cggcgttcgc cgcgctcgct gcaaccgggt
     5221 ccgacggcgc gcgctcctcg atgttacgat agaagcgatc cagcacgtcg cgcaggcggt
     5281 tcagttcttc cagcgcctgc gagaagtcga ccttgccgtt ggcggctgcc tggtagcgct
     5341 cgacgaccga ccgaatgtca tcaacctcgg cgcggtagtc gaacgggtgg actggagcgt
     5401 tcagcgatcg cagggccgcg acggcgtaga ccttgatatc acgcagcagg ttatcccggt
     5461 cggcgatttc gagtgtgtcg tcgacggtgt gccaggcgat gttgccgccg cagccggcga
     5521 cgccgtagta actcttctcc agccgcagat cttccggcat cgtcgacgag agcatgaagt
     5581 agagcgggat gccgatgttg ttgaacgaat tgtccccggc ccgcagcggc cgcaatccgg
     5641 ttgattcctg tccggtgacg tccttgacga tctggctgac caggtcggcg gccgcttcga
     5701 cccgcatcac gttgtcgtag acggttgccc aacggcagcc gggcgagtca ccgttgatgt
     5761 gggagacgca gtgctccatc aggtcgaggg cgaactcgtc cgcgtaccag gtcgagcctg
     5821 cgtagcgtcc gtgtgaatga ccgctccacc acgccagccg gagcgaccgc cgtgtgatcg
     5881 agccgacggt cgacgagcag aacatcctcg aagagacgct gttcgacgag gctgaattcc
     5941 tgaagacgtt cgggctggac cgcttcctgc tggacctgcg tggccagcaa ctcaagatca
     6001 aggacatgtt ccaccggtcc caccgaagct ggcggcggct gtgttgaact gatgggctag
     6061 ccgcagaccg ttaccccacc tgatcaacgg ccgcgccggc aatttccgtc gcccggttga
     6121 gcgcatcgac gacccggttg cgagcacgga ccatcgcaac ccacgacgtc tgccactcct
     6181 cgctgtcggt agggagggat gccagccgtg ggtacggcac gagtgagggg atcatctgcg
     6241 tctgc
//
LOCUS       DASUGJ010000378         5096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_336936, whole genome shotgun sequence.
ACCESSION   DASUGJ010000378 DASUGJ010000000
VERSION     DASUGJ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336936"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VFV93_08375"
     CDS             complement(<1..774)
                     /locus_tag="VFV93_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165395.1"
                     /translation="MLKKQIGAALAVVMLGSGAASAQRVEVESLSRMPEITGVNMSAE
                     GDFLVAIVADPKNTDRRALATWDISNIDGTKPLAVSSITPSNDRMGFIAANAIKAGKV
                     LTVASQPWTGSLSGCGEGNVTGATKTWRYKTFLTDKTIRDFDDVFASGRAIGVSTEMI
                     HCLEIAGDTLIRDLPLDPENVIVVRTDITSGQAKFSKVNLKTGKSDFLYSDDGDFNIG
                     LLDPRDGKILTKAKVEPKGNLEYDGETYILNPDTKKFDLE"
     gene            1112..4066
                     /locus_tag="VFV93_08380"
     CDS             1112..4066
                     /locus_tag="VFV93_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5165396.1"
                     /translation="MFFRGGAAIGALTLAMGSAVAQQTPAPTPPSSQTQPAVDQTTPA
                     QQQGQGDRVVVTGSRIARDAFTSTAPIQVITAEASTLEGLVDTTEILQGSSIAQGSVQ
                     FNNTFGNFVIEGGPGINSVSLRGLGAQRSLVLLNGQRPGPAGVRGQVAAFDLNVMPDS
                     IINRVEILKDGASSIYGSDAVAGVVNIITRSSVDSPELTVQYNATEQGGGNTLQVTGA
                     IGWDVWGGNIMLAAEYEDRDPLKWGDRRNLSCAQDLIYDATTGQRIDRLDRSVIGGTS
                     LGGCTTGNLYHNTVIDAVGGARYVPSPNGVTTGPFPGYRPRSNRTYLNSPDGLAYFED
                     VLNSPKFADGYAISAVERTSLYGKADFNIFGGVDWTTEVLLNRREFEQKSWRQFFPQI
                     ANNAWYDVPGAYQAPATFVTTTIAGIPTRVAQPVTIWPSNDSVTVDYGYINSGLTGDF
                     FLPGWTWQLNASYSMSDGEYSGNEILKETAGDWNYTSPSDGLYHGPNYNPFDPAFLSG
                     NYSDAVFDMLTGYSVGSTTYEQTVVSGVLTGDVFELPAGPLGLAVGAEWRNFEIDDTP
                     DERSQNDEFWGSTSALVTRGEDTVTEAFAEVNVPILKGVPFFEELTLDGSVRAFDYDS
                     YGSDSVWKAGLNWQVIPSVRLRGTLGTSYRAPALYELFLGNQTGFLSQASIDPCINWG
                     NSTNPDIQANCASEGIPANYTGLGSSATIISGGGAGVLQAETSEASTLGVIWTPDFID
                     LSLAIDYFDITIDDEVAQLGASAILGGCYGSPVFPNAFCSLFTRNPGTTPGAAWNITT
                     VMDSYINVNNQSTRGIDITLRYEHEFNFGDLSVDLSATQTLEDVVNLFDPSVVSGFET
                     NDFNGSIGDPEWVGDATIQLRRGDFTYSWFIDYIGKTDNRVLGIDEVIPYQGRTGRRI
                     ITTDDWLSHDVSVRWRGDKVTVTGGVANLFDAQPPVVSNGAAQRLQNYALAATQYDLR
                     GRTFFLRVGYEF"
     gene            complement(4140..>5096)
                     /locus_tag="VFV93_08385"
     CDS             complement(4140..>5096)
                     /locus_tag="VFV93_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5165397.1"
                     /translation="AEDVALVTGPLYHAAPLGINMVIPINAGVTVLLQDKWDAEDTLR
                     LIEQYKATHTHVVPTMMHRMLQLPEETRKKHDVSSMRWIMHGAAPCPAHVKKAMMDWF
                     GPVIHEYYSSTEGGGVYVGPHDWLKKPGTVGKAVPGVEIRLLDNDGNPVPQGQEGLIY
                     VKAPDVGRFEYYKEPAKTESSYRDNWFTLGDMGRLDADGDLFLTGRTAELIISGGVNI
                     YPVEIDEVLIRHEGIADAAVVGVPNEDWGEEIKAVVELKPGWRASSETAQSILDFAKD
                     KLPGFQRPRTIDFVDALPRNAAGKVLRAQVRAPYWKGRSKSI"
BASE COUNT          961 a         1590 c         1525 g         1020 t
ORIGIN      
        1 ttcgaggtcg aacttcttcg tgtccggatt gaggatgtag gtttcgccgt catactcaag
       61 attgcctttc ggctcgactt tggcctttgt gaggatcttg ccgtcgcgcg ggtcaagcag
      121 gccgatgttg aagtcgccat cgtcgctgta gaggaaatcg ctttttccgg ttttgagatt
      181 gaccttcgag aacttggcct gaccggacgt gatgtccgta cgaacaacga ttacgttttc
      241 ggggtcgagg gggagatccc ggatcagtgt atccccggca atctcaaggc agtgaatcat
      301 ctccgtgctg acaccgatcg cgcggcccga cgcgaacacg tcgtcgaaat cgcgaatggt
      361 cttgtcggtg aggaaggtct tgtagcgcca ggtcttggtg gcgccggtga cattgccttc
      421 cccgcacccg gacaaagagc cggtccaggg ttgggatgcg acggtcagga ccttgccggc
      481 cttgatggcg ttggcggcaa taaaacccat ccggtcgttg ctgggcgtga tcgaggaaac
      541 cgccagcggc ttggtgccgt cgatattcga aatatcccac gtcgcgaggg cgcggcggtc
      601 ggtattcttg ggatcggcca cgatggcgac cagaaaatcg ccctccgccg acatgttcac
      661 accggtgatt tccggcatgc gggagagaga ttccacctcg acccgttggg cagacgctgc
      721 cccggaaccg agcatcacaa ccgccaaagc ggcgcctatc tgtttcttga gcaaattgcg
      781 cccctgattt tgcgtagtta acgcgaagaa tagctgcact aatgctgcgg tgttcgcctg
      841 tcacgcgcaa gaggcatcac acagacatca cagaattgga aattcctgcc ccagatgaag
      901 ggtcttcttg tgacgtaact gccacagcag atttgcaatt atgtccggaa tgcggcgcga
      961 ttgttgaagt ttggacagtg accgtccaga acggccaagg agcccgagtc aggacgggca
     1021 gggactagtt ctaagcgccg aagtcaggat cggcgtcgtt tcaatcggca ttctggggtc
     1081 catcatgtcc aatagaggcg catctctgcg catgtttttc cgtggcggcg cggctatagg
     1141 cgcgctcact ctggctatgg gttcggcggt tgctcagcaa actccggctc ctacaccacc
     1201 ctcgtcacag acacagccgg ccgtggatca gaccacgcct gctcagcaac aaggccaggg
     1261 cgaccgcgtc gttgtcaccg gttcgcgcat tgcgcgcgat gccttcacct cgacagcccc
     1321 gatccaggtg atcacagcgg aagcctccac gctggaaggc ctcgtggaca cgaccgaaat
     1381 cctgcagggc agctcgatcg cccagggttc ggttcagttc aacaacacct tcggcaactt
     1441 tgtcatcgaa ggcggccccg gcatcaactc ggtctcgctc cggggcctcg gcgcccagcg
     1501 atcgctggtt ctgctcaacg gccagcgtcc gggccccgcc ggcgtccgtg gtcaggtggc
     1561 ggcgttcgac ctcaacgtca tgcctgactc gatcatcaac cgtgtcgaaa tcctgaaaga
     1621 cggcgcctcg tcgatctacg gctcggacgc tgtcgccggc gttgtgaaca tcatcacccg
     1681 gtcttcggtc gactcgccgg aactgacggt tcaatacaac gcgaccgagc agggtggcgg
     1741 caacacgctt caggtgactg gcgcgatagg ctgggatgtc tggggcggca acatcatgct
     1801 cgccgccgag tacgaggatc gcgatccgct gaagtggggc gatcgccgga atctctcgtg
     1861 cgcccaggac ctgatctacg acgcgacgac cggtcagcgg atcgatcgcc tcgaccgttc
     1921 ggtcatcggc ggaacatcgc ttggtgggtg cacgaccgga aacctgtacc acaacacagt
     1981 catcgacgcc gtgggcggcg cccgatatgt gccctcgccc aacggggtta ccacgggacc
     2041 gttcccgggc tatcgccccc gtagcaaccg gacatatctg aactcgcccg atggcctcgc
     2101 ctatttcgag gacgtcctca actcgccgaa atttgccgac ggctacgcaa tatcggcggt
     2161 ggagcgcacc agcctctacg gcaaggcgga cttcaacatc tttggcgggg tcgactggac
     2221 gacagaagtg ctgctcaacc ggcgcgagtt cgaacagaag tcctggcgtc agttcttccc
     2281 gcagatcgcc aacaacgcct ggtacgatgt gcccggcgcg taccaggccc cggccacatt
     2341 cgtcacgacc accattgccg gcatcccgac ccgcgtcgca cagccggtca ccatttggcc
     2401 gtcgaatgac tctgtgacgg tcgactacgg ctacatcaat tccggtctga cgggagactt
     2461 cttcctgccg ggatggacct ggcaactcaa cgcctcctac tcaatgtcgg acggcgaata
     2521 cagcggaaac gaaatcctga aggagacagc gggcgactgg aactatacga gcccgtctga
     2581 cggcctctac cacggtccga actataaccc gttcgatccg gcgttccttt ccggcaacta
     2641 ttcggatgcg gtcttcgaca tgctgaccgg atattcggtt ggctcgacga cgtatgagca
     2701 gacggtcgtt tcgggcgtcc tgaccgggga cgtgttcgag ctgccggcag ggccactcgg
     2761 cctcgcggtg ggcgccgaat ggcgcaactt cgagatcgac gatacgccag atgagcgttc
     2821 gcagaacgac gaattctggg gatccacgtc ggctctcgtg actcgcggtg aagacacggt
     2881 cacggaagcc ttcgccgagg tgaacgttcc gatcctcaaa ggcgtgccct tcttcgaaga
     2941 actgacgctt gatggctcgg tccgggcctt tgactacgac tcatatggtt cggacagcgt
     3001 gtggaaggcc ggcctcaact ggcaggtcat tccgagcgtt cgcctgcgcg gtacgctcgg
     3061 tacttcctat cgtgcgcctg cgttgtacga actgttcctt ggcaaccaga ccggcttcct
     3121 cagccaggct tcgatcgatc cctgcattaa ctggggcaac agcaccaacc ccgacatcca
     3181 ggcgaactgc gcctcggaag gcattccggc caactatacc ggcctgggct catccgcgac
     3241 gatcatctcg ggtggtggcg ctggcgttct ccaggccgag acctcggaag cgagcactct
     3301 cggtgtgatc tggacgccgg acttcatcga tctgagcctt gcgatcgact acttcgacat
     3361 cactatcgac gatgaagtcg ctcagctggg cgcttcggcc atccttggcg gatgctatgg
     3421 atccccggtg ttcccgaacg cgttctgcag cctgttcacc cgcaaccccg gcacaacgcc
     3481 cggagctgcc tggaacatca ccaccgtgat ggacagctat atcaacgtga acaaccagtc
     3541 gacgcggggc atcgatatca ccctgcgtta cgagcacgag ttcaacttcg gcgacctgag
     3601 tgtcgacctg tcggccacgc agacgctgga agacgtcgtc aacctcttcg acccgagcgt
     3661 cgtctcgggc ttcgagacga acgacttcaa cggctcgatc ggcgatcccg agtgggtcgg
     3721 cgatgcgacg atccagcttc gtcgcggtga cttcacctat tcctggttca ttgactacat
     3781 cggcaaaacc gacaaccggg tcctcggcat cgacgaagtg atcccctatc agggccgcac
     3841 cggccgccgg atcatcacga cggacgactg gctgtcgcac gacgtgtcgg ttcgctggcg
     3901 cggagacaag gtcaccgtca cgggcggcgt ggccaacctc ttcgacgcgc agccgccggt
     3961 ggtttcgaac ggcgctgcgc agcgcctgca gaactacgcg ctcgcggcga cgcagtacga
     4021 cctgcgcggc cgtaccttct tcctccgcgt tggctacgag ttctaagtca cgctggcaga
     4081 cagaaatgga aagggcggcc ctcgggccgc cctttttgtt tgttcgtttg gaacccgctc
     4141 tagatgctct tcgaccggcc cttccagtag ggcgcgcgaa cctgcgcgcg gagcaccttg
     4201 cctgcggcat tgcggggcag ggcgtcgacg aagtcgatcg tgcgcggccg ctggaaacca
     4261 ggcagcttgt ccttggcgaa gtcgaggatg gattgagctg tctcggaaga cgctctccag
     4321 cccggcttca gctcgaccac ggccttgatc tcttcacccc agtcttcgtt ggggacgccc
     4381 accacggcgg catcggcaat gccctcatgg cggatcagca cctcgtcgat ctcgaccgga
     4441 tagatgttga ccccgccgga gatgatcagc tccgccgtcc ggccagtgag gaacaggtcg
     4501 ccgtcggcat ccagccggcc catgtcgcca agtgtgaacc agttgtcgcg atagctcgac
     4561 tccgtcttcg ccggctcctt gtaatactcg aagcggccaa catcgggcgc cttcacatag
     4621 atgaggcctt cctggccctg cgggacggga ttgccatcat tgtccagcaa cctgatctcg
     4681 acgcccggta cggccttgcc gacggtgccc ggcttcttaa gccagtcatg cgggccgaca
     4741 tagacgccgc ctccttcagt tgacgaatag tactcgtgaa tgaccggtcc gaaccaatcc
     4801 atcatcgctt ttttgacatg agcagggcag ggggcagcgc cgtgcatgat ccagcgcatg
     4861 gaggagacgt cgtgcttctt ccgggtctct tcaggcagct gaagcatgcg gtgcatcatg
     4921 gtgggcacga catgcgtatg cgtcgctttg tattgctcga tgaggcgaag cgtgtcctcg
     4981 gcgtcccatt tgtcctggag aagaacggtg acgcctgcgt tgatcgggat caccatgttt
     5041 attccgagcg gcgcggcgtg gtagagcggg ccggtcacca aagcgacgtc ttccgc
//
LOCUS       DASUGJ010000379         3635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1170155, whole genome shotgun sequence.
ACCESSION   DASUGJ010000379 DASUGJ010000000
VERSION     DASUGJ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170155"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFV93_08390"
     CDS             <1..763
                     /locus_tag="VFV93_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5165398.1"
                     /translation="FGVAAAVSHDVIREVAREAEQLGYTSFWCNDTPGADGLAALAEA
                     AAVTTTITLGVGVIPLDRRPADAIAADVAALGLPQARLLLGVGSGGDRRVSNVRAGIE
                     RLHELVSSPVIVGALGPKMTQVSGEVGDGVLFNWQTPATAQSSGQICRDAAEAAGRPR
                     PRVMAYVRTALLPQADQRLDIEAGRYGRVPAYAAHFARMGVSARETAVSGPDAASLQP
                     QIAAHEASLDETIVRAITADDSAASILELLRACAP"
     gene            839..1186
                     /locus_tag="VFV93_08395"
     CDS             839..1186
                     /locus_tag="VFV93_08395"
                     /inference="COORDINATES: protein motif:HMM:NF021119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HEX5165399.1"
                     /translation="MQASEFIDSARDAMTVKRVFGEPFEKDGVTIIPAAKIGGGAGAG
                     SGDGEDGAGSGGGFGLSAKPAGAYVVRDGDVRWEPAIDVNRIVLGAQIVAVILALVIG
                     SIVRSRAKAAVNH"
     gene            1268..2332
                     /locus_tag="VFV93_08400"
     CDS             1268..2332
                     /locus_tag="VFV93_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5165400.1"
                     /translation="MAWNVGVLGLTHDHVWEHIGTLAQRADVRLSVADPNAPLVEKVR
                     DEYGVERGYDDYAALLERERPDAVLVFADNAGSIEIVDLAASHGTPVMLEKPMADALA
                     GAERIRVAMNKAAVPLMVNWPTNWTPAVRHALDLAASGAVGDIFRFSFRGGHAGPKEY
                     GCSEYFYSWLYDRERNGAGAYIDYCGYGAVMARQLLGRPARAQATIGRLQKDYVPVDD
                     NAILVLRYPQAMAVIEATWTANGPVPDGGPAIWGSAGTLVVQRQSARREGQIVRTGVV
                     KHITRDDPDGEMIDPPDLPEGARNATEYFLNCLAADRPFEGLVSLDTSLGAQEILEAG
                     LIAARAGSEVSLPLDICNWG"
     gene            2390..>3635
                     /locus_tag="VFV93_08405"
     CDS             2390..>3635
                     /locus_tag="VFV93_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5165401.1"
                     /translation="MSNPIRIGVIGCGRILPAHFRGLMRLREAGYDGFRVTGLVARNP
                     DDARRFRKRGEGPAPRPPVARDPNDSLAAPHSYVSDWQPEQDAQIFDSIEAILAADAV
                     DAVTITASLPVHHTATLQALSAGKHVMVEKPLAVSVRAGRQMVDAADANGLTLGVMEM
                     VRYQPATRMNHWLIERGDLGDVQMVVAVSIGTSEWSPDRVVADTPWRHDRLTAGGGAS
                     IDIGVHLSHRLRYLVGEVETMTAVARVFDPVRTVRDADGNVFGTVRADADDAFFAIPE
                     FAGSATGTISFTWAGHGEPTGLPGGLTIYGTRGCLKGTTLIRDDGSREDVADIFAREA
                     SEADRERYFPRGVTDAFGLTYLDWLNAIASGSHPETSGADALRDLATAFSIMESATAR
                     RPIAVNDVLSGEVNAYQREIDEH"
BASE COUNT          570 a         1238 c         1241 g          586 t
ORIGIN      
        1 attcggtgtt gcggcggcgg tcagccacga tgtcatccgc gaggttgccc gcgaggcgga
       61 acagctcggc tacacgtcgt tctggtgcaa tgacacgccc ggcgcggacg gactggcggc
      121 cctggccgaa gcggcggccg ttacaacgac gatcacgctc ggcgttggcg tcatcccgct
      181 cgatcgcagg ccggccgatg cgatcgctgc cgatgtcgcc gcgcttggcc tcccgcaggc
      241 ccggctgttg ctcggcgtcg gctccggcgg cgaccggcgc gtcagcaacg tccgcgcggg
      301 gatcgagcgc ctgcacgagc tggtctcgtc gccggtcatt gtcggcgcgc tcggcccgaa
      361 gatgacacag gtctccggcg aggttggcga tggtgtgctg ttcaactggc agacgccggc
      421 gacggcgcag agttcggggc agatctgtcg cgacgccgcc gaagctgccg gtcggccgcg
      481 cccgcgcgtg atggcctacg tccggacggc gctgctgcca caggccgatc agcggctcga
      541 catcgaggcc ggccgctacg ggcgcgtccc ggcctacgcg gcccacttcg cccggatggg
      601 cgtctccgcc cgcgaaacag ccgtcagcgg gccggatgcc gccagcctcc agccacagat
      661 cgccgctcac gaggcgtcgc tcgatgagac gatcgtccgc gcgatcactg cagacgacag
      721 cgccgccagt atcctggagt tgctgcgcgc ctgcgcgccg tagaatggtg gcacggctga
      781 tgccgcgacc aaccgttcga tctgacccgg cgacgtgtta cagcggaggg agttcaggat
      841 gcaggcttca gaattcatcg attccgcgcg cgatgcgatg acagtcaagc gggtcttcgg
      901 cgagccgttc gagaaggacg gtgtcacgat cattccagcc gcgaagattg gtggcggcgc
      961 cggtgctggc agcggcgatg gtgaggatgg cgccggcagt ggtggcggct tcggtctgtc
     1021 ggccaagccg gccggcgcct atgtcgttcg cgacggggat gtccgttggg aaccggccat
     1081 cgatgtcaac cggatcgtcc tcggcgccca gatcgttgcc gtgattctgg cgttggtcat
     1141 cggctcgatc gttcgcagcc gcgcgaaggc tgccgtaaac cactgacccg ttcgtcgtcc
     1201 ctgttgccat ctccaggcag atgaccgatg atcccactcg cgaaccgcga atgggggagg
     1261 gcatcagatg gcctggaacg ttggcgtcct gggattgacg cacgatcacg tctgggagca
     1321 catcggcacc ttggcgcaac gggccgatgt gcggctctcg gtcgctgatc cgaacgcacc
     1381 actggtcgag aaggtccgcg acgagtacgg cgtcgagcgt ggctacgacg actacgccgc
     1441 gctgctggag cgggagcggc ccgacgcggt cctcgttttc gccgacaacg ccggctcgat
     1501 cgagatcgtc gatctggccg cctcgcacgg cacgccggtc atgctggaga agccgatggc
     1561 cgatgcgctg gcgggcgccg agcggatcag ggtcgccatg aacaaggccg ctgtcccgct
     1621 gatggtcaac tggccgacga actggacgcc ggctgttcgc cacgcgctcg atctggccgc
     1681 cagcggcgcg gttggcgaca tcttccgctt ctccttccgg ggtggtcatg ctggaccgaa
     1741 ggagtatggc tgctcggagt atttctactc ctggctctac gaccgcgagc gtaacggggc
     1801 cggcgcctac atcgactact gcggctacgg cgctgtcatg gctcgccaac tgctcggccg
     1861 gccggcgcgc gcccaggcaa ccatcggccg gctccagaag gactacgttc cggtcgatga
     1921 caacgctatc ctcgtcttgc gctatcccca ggcgatggcc gttatcgaag cgacctggac
     1981 cgcgaacggc ccagttcccg atggtggccc ggccatctgg ggcagcgctg gcacgctggt
     2041 tgtccagcgc caatccgcgc gccgcgaggg ccagatcgtc cgaactggcg tcgtcaagca
     2101 catcacgcgg gatgatccag acggtgagat gatcgacccg cctgacctgc ccgaaggcgc
     2161 ccgcaacgcg accgaatact tcctgaactg cctcgccgcc gaccggccgt ttgagggtct
     2221 cgtctcgctc gacaccagcc tcggcgccca ggagatcctc gaagccggcc tgatcgccgc
     2281 ccgcgccggc agcgaggtct cgctgccgct ggatatctgc aactggggat gaaatgcgtg
     2341 acgcgtgata cgtgatgcgt gacgcgggaa gggctgtgga ggaagaagcg tgtccaaccc
     2401 gatccgaatt ggcgtcatcg gctgcgggcg gatactgccc gcacactttc gcggcctgat
     2461 gcgcttgcgt gaggcaggtt atgacgggtt ccgcgtgacc ggactggtcg ctcgcaatcc
     2521 cgacgacgcc cgccgcttcc gcaagcgggg tgaagggcca gcgccccggc cgccggtcgc
     2581 gcgcgacccc aacgactcgc tggcggcgcc acactcgtac gtcagcgact ggcagcctga
     2641 gcaggacgcg cagatcttcg actcgatcga ggcgatactg gcggccgatg ccgtcgacgc
     2701 tgtcacgatc acggcctcgc tgccggtcca ccacacggcg acgcttcagg cattgtcggc
     2761 cgggaagcac gtcatggtcg agaagccgct ggcggtcagc gtcagggccg gtcgtcagat
     2821 ggtcgacgca gcggacgcga acggtctgac gctcggcgtg atggagatgg tccgctacca
     2881 gcccgcgacg cggatgaatc actggctgat cgagcggggc gacctgggcg acgtccagat
     2941 ggtcgtggcc gtctcaatcg gcaccagcga gtggtcgccc gaccgggtcg tcgccgacac
     3001 gccctggcga cacgaccggc tgacggccgg cggcggcgcc tcgatcgaca tcggcgtcca
     3061 cctcagccac cggctgcgct acctcgtcgg tgaggtcgag acgatgacgg ccgttgcgcg
     3121 cgtcttcgac ccggtccgca ccgtccgcga tgccgacggc aacgtcttcg ggacggtccg
     3181 cgccgatgcc gacgacgcct tcttcgcgat accggagttc gccggtagcg cgaccggcac
     3241 gatctcgttt acctgggccg gccacggcga gccgaccggc ctgccgggcg ggctgaccat
     3301 ctatggcacg cgcggctgcc tgaaaggcac gacgttgatc cgcgacgacg gctcacggga
     3361 ggacgtcgcc gacatcttcg cccgtgaggc gagcgaggcc gaccgcgagc gctacttccc
     3421 tcgcggcgtg accgatgcct tcggcctgac ctacctcgac tggctcaacg cgatcgccag
     3481 cggcagccat cccgaaacca gtggcgctga cgcccttcgc gacctggcga ccgccttctc
     3541 gatcatggaa tccgccaccg cccgccgccc catcgccgtc aacgacgttc tcagcggtga
     3601 ggtcaacgca taccagcgcg agatcgacga gcatt
//
LOCUS       DASUGJ010000380         4093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_893156, whole genome shotgun sequence.
ACCESSION   DASUGJ010000380 DASUGJ010000000
VERSION     DASUGJ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893156"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFV93_08410"
     CDS             <1..522
                     /locus_tag="VFV93_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165402.1"
                     /translation="EFLAASDPSVLAGDLNRLLTDESQRVRAIAWDAVLRLDRPAAFA
                     RASDILSDESAPIVVRRSALVALGALLPTAEMAPLLAYFVVHPDHELAADAADLLYER
                     HRNPLAAMAARDSPHADVREVAEQLLDPMRGSPAAGGSRPGDPTGSSAGIYSDMIRQL
                     EEQTENQSDPTER"
     gene            612..1142
                     /locus_tag="VFV93_08415"
     CDS             612..1142
                     /locus_tag="VFV93_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HEX5165403.1"
                     /translation="MSDETPTATLDTDERLVTRYNEVELNCPRCRARWSPVVADVINF
                     GTDPKGREGILRKSIHHSYCPACKYHMEIDHIFVVYDPAENLLLQVRPGWELPAGGGE
                     EYYWTRLEDAILKYQDLDLRVDVVFGYDQLIEKYLGGQPAVDAALQRAAQEREQQLAS
                     GSLAPGVAPPEGIRTA"
     gene            1308..2552
                     /locus_tag="VFV93_08420"
     CDS             1308..2552
                     /locus_tag="VFV93_08420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165404.1"
                     /translation="MSRPPQAGPARRRSRRKRGRFTDRHLLVGEGFFSGTATATHNAF
                     FVTLLVQLNVSSLALGIYTSLNGLLANASGLAGGRLRRHIPNRRTLAAVTGGLGRLGF
                     LLIAILLFLQGADVNLTALIVIALVTVSLIGLGLPVLTSVIADSVNQRERGFFFANRL
                     LASGVGATIVAIGVAALLRQLTFPNGFMIAYLIAAVAGLGSVVCLLSLRRVRNEPVTG
                     TRGAAGFGLFGSVSPLMWRYAGSTFILWFGAALIAPVLTPYILNDLNAAPSFIGLMTA
                     VNATTGLLIQRFWGRRVDRFGSYPVIAVTMAAVSSLPVLYALTPTYWLGLGFEVISGV
                     AWAGYALGNLNYAIEIAPAEDRARYTSVGNAAAGIGSFLGPLVAALLLTFLEPRVLLI
                     LSGAIRFSAFLSVRFARPPARD"
     gene            2607..3953
                     /locus_tag="VFV93_08425"
     CDS             2607..3953
                     /locus_tag="VFV93_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5165405.1"
                     /translation="MPNDLMGRIDWNSVRDEVTQHLQTLIRFETVNPPGNEALVAGYL
                     KEMVEKAGLSATIVEKTSGRGNFVTRVKSTGNGRPLLLMAHADVVSVEADKWTHPPFE
                     AGIHDGMIWGRGAVDTKNLVASELMVMLLLARNGLTLDRDVIMCTFADEEAGGRDGAD
                     FMWQEHRELIDAEVAINEGGGQAVDVMGKRFYMCQTGEKGGARMTLVASAPPGHASVP
                     RDDTAMLRMGRALVKLSQHQFPTIITASVERMLLAMGESIGGDATELVNDILSDPSWE
                     NLSKLPLGEVERLSLRATTRNTAVPTIIHGGHRINVIPGEITCDVDGRVLPGQNPADF
                     VQQVRDLIGDDVEVRAHGNGSSGIEADPDSALFETIREVMGEIDPGSEVAPFLVSGGT
                     DAKSIPGIKVYGFNPGRFSDVEMNGAHNHDERVSIDNLEFATRALFDITTRYSAAR"
BASE COUNT          695 a         1457 c         1247 g          694 t
ORIGIN      
        1 gagtttctgg ccgccagcga cccgtccgtc ctggccggcg acctcaaccg gctgctgacc
       61 gacgagagcc agcgtgtcag ggcgatcgcc tgggacgctg tcttgcgcct cgaccggccg
      121 gccgctttcg cccgcgcatc ggacatcctc agcgacgaat ctgcgccgat cgtcgttcgg
      181 cgcagcgcgc tggtcgcgct aggggcactg ttgccaacgg ccgagatggc accgctgctg
      241 gcctacttcg tcgtgcatcc cgaccacgag ctggccgccg atgccgccga cctgctctac
      301 gagcgccacc gcaacccgct ggcagcgatg gccgcccgcg acagcccgca tgccgatgtc
      361 cgcgaggtcg ccgagcagct gcttgacccg atgcgcggct caccagcggc aggtggctcc
      421 cgccctggcg atccgaccgg atcatcagcc ggcatctact ccgatatgat tcgccagctt
      481 gaggagcaga ctgaaaacca gtctgaccca accgagcgtt agtctttacg gttgaagcgt
      541 gcgacctcgc ttcgccatac ttttggcgca tatcgttcaa cctgatgaag acgaagggtt
      601 tcagcagtct catgtcagac gaaacaccaa cagcaacact cgacaccgac gaacggctcg
      661 tgaccaggta caacgaggtc gagctcaact gcccgcgctg ccgcgcgcgc tggtcgcctg
      721 tcgtcgccga cgtcatcaat ttcggcaccg atccaaaggg ccgtgagggc atcctgcgca
      781 agtcgatcca ccactcgtac tgcccggcct gcaagtacca catggagatc gaccacatct
      841 tcgtcgtcta cgacccggct gagaacctgc tgcttcaggt tcgcccaggc tgggagctcc
      901 cggccggcgg cggcgaggag tattactgga cccggcttga ggacgcgatc ctcaagtatc
      961 aggatctcga tctgcgcgtc gatgtcgtct tcggatacga ccagctgatc gagaagtacc
     1021 tcggcggcca gccggccgtc gacgccgccc tccagcgcgc ggcccaggaa cgcgaacaac
     1081 agcttgcctc cggctcgctc gcccccggcg tcgccccgcc cgaaggcata cgcacggcct
     1141 gactgccgct ccgattcggg gatgagggat gaggcccgag ggatcagcac gcatacagcg
     1201 tccggctcat ccctcgggcc tcatccccat tgtcgagatg gcgtgtcgca cgtacgatat
     1261 cgatgagaca gccgatccgc tgaacgaaac acaggagcac tcgccacgtg tcgcgaccgc
     1321 cgcaggctgg tccagcaaga cgacggtcaa gacgcaaacg gggacggttc accgaccggc
     1381 atctgctggt cggcgagggc ttcttctccg ggacggccac cgccacccac aacgccttct
     1441 tcgtcaccct gctcgtccag ctcaacgtca gcagcctggc gctcggcatc tacacctcgc
     1501 tgaacggcct gctggccaac gcctccggcc tggctggtgg caggctgcgt cgccacatcc
     1561 ccaaccggcg cacgctggcg gccgtcaccg gcggcctcgg ccggctgggc ttcctgctga
     1621 tcgcgattct gctgttttta cagggcgcgg atgtcaacct gaccgcgttg atcgtcatcg
     1681 cgctggtgac ggtctcgctg atcgggctcg ggctgccggt cctgacgtcg gttatcgccg
     1741 actcggtcaa ccagcgcgaa cggggcttct tcttcgccaa ccggctgctg gccagtggcg
     1801 tcggggcgac gatcgtcgcg atcggggtcg ccgcactcct gcgccaactg acctttccca
     1861 acggcttcat gatcgcctac ctgatcgcgg ccgtcgccgg gctagggtcg gttgtctgcc
     1921 tgctctcgct gcggcgggtg cgcaacgagc cggtcaccgg cacgcgtggc gcggccggct
     1981 tcggtctgtt cggctccgtc tcgcccctga tgtggcgcta cgccggttca acgttcatcc
     2041 tctggttcgg cgcggccctg atcgcaccgg tcctgacgcc gtatatcctc aacgacctca
     2101 acgctgcccc gagcttcatc ggcctgatga cagccgtcaa cgcgacgacc ggcctgctga
     2161 tccagcgctt ctggggccgc cgcgtcgatc gcttcggctc gtatccagtc atcgccgtca
     2221 cgatggcagc cgtctccagc ctgcctgtcc tctatgcgct gacgccgacc tactggctcg
     2281 gcctcggctt cgaggtcatc tccggcgtcg cctgggccgg ctacgcactg ggcaatctca
     2341 actacgcgat cgagatcgca ccagccgagg atcgtgcccg ctacacctcg gtcggcaatg
     2401 ccgcagccgg catcggctcg ttcctcggcc cgctcgtcgc cgccctgctg ctgacctttc
     2461 tggagccgcg cgtgctactg atcctgtcgg gtgcgatccg cttcagcgcg ttcctgtccg
     2521 tacgcttcgc gcgaccaccg gcgcgcgact gaactggtgt atcctgccgc gggggaagaa
     2581 ggacaaacga aggagcaacc tgaaccatgc caaacgatct catgggacgt atcgactgga
     2641 acagtgtccg cgacgaagtg acccagcacc tccagaccct gatccgtttc gagaccgtca
     2701 acccgcccgg caacgaggcg ctggtcgccg gctacctcaa ggagatggtc gagaaggcgg
     2761 gcctcagcgc taccatcgtc gaaaagacct ccggccgcgg caacttcgtc acccgcgtca
     2821 agagcaccgg caacggcagg ccgctgctgc tgatggccca cgccgatgtc gtctcagtcg
     2881 aggccgacaa gtggacgcat ccgccgttcg aagccggcat ccatgacggc atgatctggg
     2941 gccgcggcgc cgttgatacc aagaacctgg tcgcctcgga actgatggtc atgctgttgc
     3001 tggcccgcaa cggcctgacg ctcgaccgcg atgtcatcat gtgcacgttc gccgacgagg
     3061 aagccggtgg ccgtgacggc gccgacttca tgtggcagga gcatcgtgag ctgatcgacg
     3121 ccgaggtcgc catcaacgag ggcggcggtc aggccgtcga tgtgatgggc aagcgcttct
     3181 acatgtgcca gaccggcgag aagggcggcg cgcggatgac gctggtcgcc agcgccccgc
     3241 cgggacacgc ctcggtcccc cgcgacgaca cggccatgct gcgaatgggc cgcgcgctcg
     3301 tcaagctctc ccagcaccag ttcccgacca tcatcactgc ctcggtcgag cggatgctac
     3361 tggcgatggg tgagtcgatc ggcggcgacg cgacagagct cgtcaacgac atcctctccg
     3421 atccgagctg ggagaacctc agcaagctgc cgctcggcga ggtcgagcgc ctcagcctgc
     3481 gcgccacgac ccgcaacaca gccgtcccaa ccatcatcca cggtggccac cgcattaacg
     3541 tcatcccagg cgagatcacc tgcgacgttg acggccgcgt cctgcccggc cagaacccag
     3601 ccgatttcgt ccagcaggtc cgcgatctga tcggcgacga tgtcgaggtc cgtgcccatg
     3661 gcaacggttc gtccgggatc gaggccgacc ccgactcggc gctgttcgag acaatccgcg
     3721 aagtgatggg tgagatcgac cccggcagcg aggtcgcgcc gttcctcgtc tccggtggaa
     3781 ccgacgccaa gtcaatcccc ggcatcaagg tctacggctt caaccccggc cgcttctcgg
     3841 acgtcgagat gaacggcgcc cacaaccacg acgagcgcgt cagcatcgac aatctggagt
     3901 tcgccacccg cgccctgttc gacatcacga cccgctacag cgcagcgcgc tgatgacggc
     3961 gctgggacgg gccaacccgc taatcctctt cgacatcgac gggacgctgc tgcgaaccgg
     4021 cgacccgcac cacggcgcgg cctttacgta cgccttcgag tcgatctacg gcaagccggt
     4081 gacactcgaa ggc
//
LOCUS       DASUGJ010000381         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1170648, whole genome shotgun sequence.
ACCESSION   DASUGJ010000381 DASUGJ010000000
VERSION     DASUGJ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170648"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1421
                     /locus_tag="VFV93_08430"
     CDS             231..1421
                     /locus_tag="VFV93_08430"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5165406.1"
                     /translation="MQFAERMSRLGTESAFDVLVRAKALEAQGRHIVHLEIGEPDFDT
                     PAHIVEAGIAALRAGETHYTPAAGIPQLREAIAEYTSLTRGVELAPERVVVTPGAKPI
                     MFFVIQALAGEGDEVVYPDPGFPIYESVINYAGATAVPLRLSEERGFSFAPEELAGLV
                     SERTSLVIINSPHNPTGGTISPEALDELARLANEYDFMVLSDEIYARMLYDGAHESII
                     TRPGMLERTIILDGFSKTYAMTGWRLGYGVMPLEMAEQVTRLAVNCNSCVAAFTQIAG
                     VAALTGPQEPAEAMIAEFRRRRDAVVAGLNEIPGVTCRTPAGAFYVFPNISSFGRSST
                     EIASLLMDEGGVSLLAGTAFGAAGEGYLRLSYANSLANLELALERIGNTLAGVRDSGT
                     GIRG"
     gene            1523..2185
                     /locus_tag="VFV93_08435"
     CDS             1523..2185
                     /locus_tag="VFV93_08435"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5165407.1"
                     /translation="MSIQGYAKLAVFMGGVVGLGVELAAERLLAPAFGTTLDLWSIII
                     GLTFTALSAGYEIGGRIIDRRPTHRVLSVCLLIAGGWSLLISLLGRTIVDAIQELTFD
                     FGGLRLGIFLSVLLLITVPPFVIGIITPASIRLVVPRVGAAGSSAGTIYALGTIGSLL
                     GTFLPVIVLIPRIGVRFTFLSMAIVGLASGAIGLTNLVRNSATELVANTVEDPAGERV
                     AQ"
BASE COUNT          370 a          781 c          734 g          409 t
ORIGIN      
        1 ctgaacagcg tgcgcagcga acgaggatcc ccaacacctg cgcccgttaa tcaaccgccc
       61 atgttgttga gcgggcgcct cacggcgtgg accgatacag gatcggcccc tacacggtgg
      121 acgccatatt caccgtggtg gactactcag gacaatcggc taatggatct gcccggatac
      181 ggtatcgtta cgcggtctga gcgccatggg acggggagag ggagcaccag atgcagtttg
      241 ccgagcggat gagccggttg gggaccgaga gtgcctttga tgtgctcgtt cgggcgaagg
      301 cgctcgaagc tcagggccgg cacatcgtcc acctggagat cggcgagccg gatttcgaca
      361 caccggccca catcgtcgag gccgggatcg ccgccctgcg tgccggtgag acgcactaca
      421 cgccggcggc cggtattcca cagttgcgtg aggcgatcgc tgagtacacc tcgctgaccc
      481 gcggcgtcga actcgcaccg gagcgggtcg tcgtcacccc cggcgcgaag ccgatcatgt
      541 tcttcgtcat tcaggcgctg gccggcgagg gcgacgaggt cgtctacccc gatcccggct
      601 tcccgatcta cgagtcggtc atcaactacg cgggcgcgac ggctgttccg ctgcgcctga
      661 gcgaggagcg aggcttctcg tttgcgccgg aggagctggc cggcctcgtc tcggagcgga
      721 catcgctggt catcatcaac tcgccgcaca acccgaccgg cggcacgatc tcgccggagg
      781 cgctcgacga gctggcgcgg ctggcgaacg agtacgactt catggtcctc tccgacgaga
      841 tctacgcgcg gatgctctac gacggcgccc acgagagcat catcacccgg cccggcatgc
      901 tggaacggac gatcatcctc gacggcttct cgaagaccta tgcgatgacc ggctggcggc
      961 tcggctacgg cgtgatgccg cttgagatgg ccgagcaggt cacgcggctg gccgtcaact
     1021 gcaactcctg cgtggcggcc ttcacccaga tcgccggtgt cgcagccctg accggcccgc
     1081 aggagccggc cgaggcgatg atcgccgagt tccgtcgccg tcgtgacgcg gtcgtcgccg
     1141 gcctgaacga gattcccggc gtcacctgcc gcacgccggc cggtgccttc tacgtcttcc
     1201 ccaacatcag cagcttcggc cggtcatcaa ccgagatcgc cagcctgctg atggacgagg
     1261 gcggcgtctc gctgctggcc ggaaccgcct tcggcgcggc cggcgagggc tacctgcgcc
     1321 tctcctacgc caactcgctg gcgaacctgg agctggcgct ggagcgaatc ggtaacacgc
     1381 ttgctggggt tcgggattcg ggaaccggga ttcgggggta agaagctggt aacgtgatga
     1441 ctcagcaaca cggagtggta catcaagtga cgacaattca tccaaccccg aatcccgaat
     1501 cccgaatccc gagccaaccg caatgagcat ccagggatat gcaaagctcg ccgtcttcat
     1561 ggggggtgtc gtcgggctgg gggtcgagct ggcggccgag cggctgctcg cgccggcctt
     1621 cgggacgacg ctcgacctct ggtcgatcat catcggcctg accttcacgg ccctctcggc
     1681 cggctacgag atcggtggcc ggatcatcga ccgccgcccg acgcaccgcg tcctcagcgt
     1741 ctgcttgctg atcgccggcg gctggtcgct gctgatctcg ctactggggc ggacgatcgt
     1801 cgacgcgatt caggaactga cgttcgactt cggcggcctg cggttgggca tcttcctgtc
     1861 ggtcctgctg ctgatcaccg tcccgccgtt cgtgatcggc atcatcacac ccgcctcgat
     1921 ccggctggtc gttccccgtg tcggcgcggc cggttcctcg gccggcacga tctacgcgct
     1981 cggcacgatc ggctcgctgc tgggcacctt cctgccggtc atcgtcctca tcccccggat
     2041 cggcgtccgc ttcaccttcc tctcaatggc catcgtcggc ctcgccagcg gcgccatcgg
     2101 cctcaccaac ctcgtccgca actctgctac tgagttggtc gccaacaccg tcgaagatcc
     2161 agctggcgaa cgtgtcgccc agtagcgcgg atgttgaatg agcagaacgt cccatccgtg
     2221 taggggtcga tcctgtatcg gccagcgccg tgaggcgcac gctgtcggac gcgtacgctc
     2281 ctcaacacct gcgc
//
LOCUS       DASUGJ010000382         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_614245, whole genome shotgun sequence.
ACCESSION   DASUGJ010000382 DASUGJ010000000
VERSION     DASUGJ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614245"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFV93_08440"
     CDS             complement(<1..478)
                     /locus_tag="VFV93_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165408.1"
                     /translation="MPSKARALLRLPRSHAVRKRAVQLLMLVLALVTLAGPILGPTGR
                     EAAAQVSDFSPPPLLSRDQLNSPDSDRYRATVDQIRSLANLEQQAVQATLHNHGLPAS
                     DADAVRSWGRADATAELWGLVVEAIQTPEASRDPDQQNVVDWLAAASKRQSVLAADY"
     gene            complement(768..>2918)
                     /locus_tag="VFV93_08445"
     CDS             complement(768..>2918)
                     /locus_tag="VFV93_08445"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5165409.1"
                     /translation="VGRVPELDEICNLLRRSRLVTLTGPGGSGKTRLSIAAARRLADE
                     FVGGVWFVDLAPLADPALVVAEVAGRLGVAGQADRSLIEDVADVLRDPSLIVLDNCEH
                     LIDTCAGLADVLLRAAASLTILATSREALRVGGEVIWPAPPLRLEVEDDPLQVEAIQL
                     FVERARLANPHFTVTDASSRVILDICRQLDGMPLAIELAAARTRVLPVEQLAARLGDA
                     LALLGGGLRTTPERQQSLRGTINWSYALLTPEEQALFRRLAVFAGGFVPEAVEEVCSG
                     EGLARDAVMELLFHLADKSLATPQEDDASARLRMMEPLRQFALERIAETELATLRERH
                     AAYFLTLAEQAAPNLTSPDATTWNTRLDTEHDNLRVALAWVVEHGDAERGLRLGRALW
                     RFWLQRGYFAEGRQWMTRMLAMEGTKMHPQPYADVLFGMGMLALFQTDFAATESHFTA
                     CLDVARAANHRVTIASAATQLSIIAQERGDDQTAERLLEEGLAIRLELGRLQEVADSL
                     TMLGEFAFVRSDIETAQTRYEESLKLQQETGDLAQRAKVLDRLAAIAITRGDLATARL
                     LLEEGLEVAEATPYRVPDLLGSLAQVAFDEGDTSEAARLAARCIRSSLEMPRKDRLAW
                     GLLLFATLASARGEGERALRLRTAAQHVLVAAGRSIAFTPILQRWIETRIEPGIVELD
                     VFARERAVRHGTAMTLDEAIADALADLPSPATTN"
BASE COUNT          517 a          941 c          951 g          509 t
ORIGIN      
        1 catagtcggc ggcaaggacg gactgtcgct tggacgcggc cgccagccag tcgaccacat
       61 tctgctggtc gggatcgcgc gacgcctctg gcgtttggat ggcttcgaca acgagccccc
      121 acaactcagc ggtcgcgtcg gcgcgtcccc agcttcgcac ggcgtcggcg tcactggcag
      181 gcaggccgtg attgtgcagg gtagcctgca ccgcctgttg ctcgaggttc gccagcgacc
      241 ggatttgatc gacagtagcc cggtagcggt ccgagtctgg tgagttgagt tgatcgcggc
      301 tcagcagtgg tggcggcgag aaatcactca cttgcgctgc cgcctcgcgt ccggtcgggc
      361 caaggatcgg cccagccagc gtcacgagcg ccaggacgag catgagcagt tgaaccgcgc
      421 gcttgcggac agcatgcgac cggggcaagc gtagcagcgc acgtgctttc gatggcatcg
      481 ttcgatctcc ctttcatccc agtcgtctgg cggacccgtg tttgaagagc aacgtcgttg
      541 ctcaatcagg ttggggcgtc gtggcgcggg cgcctcgcgg cgcacatcga gcgcgcggga
      601 gcgccgctcc tgcgaaatca gtcacgagga gagccgcatc aacatgcgac tgagcgtcgt
      661 gccgatgtcg tcgggttgcg aaagaagcgt agtgagtggt tggcaacacg tcgtcaacac
      721 aacgtcaacg gatgcgcggg atcaatcaac gcgacggaat gcggatgtca attggtggtg
      781 gcgggggacg gcaggtcggc gagggcgtcg gcgatagcct catcgagtgt catcgccgta
      841 ccgtgccgca ctgctcgttc gcgcgcgaac acgtcgagct ccacgattcc cggctcgatc
      901 cgtgtctcga tccagcgttg cagaatcggg gtgaaggcga tactgcgtcc ggctgcgacg
      961 agcacgtgtt gcgccgcggt ccggaggcgg agtgctcgtt caccctcgcc cctggcgcta
     1021 gcgagcgtgg cgaacaacaa gagtccccag gcgagtctgt ccttacgggg catctccagg
     1081 cttgaacgaa tacagcgcgc ggcgagtcgg gccgcctcgc tcgtgtctcc ctcgtcgaat
     1141 gccacctgag cgagacttcc caataggtca ggtacgcgat acggtgttgc ctccgcgacc
     1201 tccagcccct cttcgagcag cagtcgtgcg gttgcgagat caccccgagt gatcgcgatt
     1261 gctgccaggc ggtcgagcac cttggcccgc tgagccagat cgccggtctc ctgttgcagc
     1321 ttcaggctct cctcataacg cgtctgcgcc gtctcgatgt cggatcggac gaaggcgaat
     1381 tcgccgagca tcgtgagcga atcggcgacc tcctgcaggc gtcccagttc cagccggatg
     1441 gcgaggccct cttccaacag ccgctcggcg gtttgatcgt caccacgttc ctgggcgatg
     1501 atgctgagtt gtgtcgctgc gctggcgatc gtgacccggt gattcgctgc gcgcgcgaca
     1561 tccaggcagg cggtgaagtg tgactcagtc gccgcgaaat ctgtctggaa cagcgccagc
     1621 atgcccatgc cgaacaggac atcggcgtag ggttgaggat gcatcttggt cccttccatg
     1681 gccagcatcc tcgtcatcca ctgccgccct tcagcgaaat agccccgctg taaccagaaa
     1741 cgccagagcg ctcgtcccaa acggagtccc cgctcggcat cgccatgttc gacgacccag
     1801 gcgagcgcga cgcgcaggtt gtcgtgctcg gtatcgaggc gcgtgttcca ggttgtggca
     1861 tccgggcttg tcaagttcgg cgccgcctgt tccgcgagcg tcagaaagta agcggcgtga
     1921 cgctcgcgca gcgtcgcgag ttcggtttcg gcgattcgtt ccagcgcgaa ttgccgtagc
     1981 ggctccatca tccgaaggcg tgccgaggcg tcgtcctcct gcggggtggc gagcgacttg
     2041 tcagccaggt ggaagagcaa ctccatgacc gcgtcgcgag cgagtccttc accagagcag
     2101 acttcctcaa ctgcttcggg aacaaatcca ccggcgaaga cggccaggcg gcggaagagt
     2161 gcctgctcct cgggcgtgag tagcgcgtag ctccaattga tcgtgccacg cagcgactgt
     2221 tgacgctccg gggttgtccg caatccaccg ccgagtaacg cgagggcatc tccgagacgt
     2281 gcggcaagtt gctcgaccgg caggacgcgc gtcctggccg ccgccaactc gattgccagg
     2341 ggcattccgt ccaactggcg gcagatgtcc agaatgacgc gcgacgatgc atccgtcacc
     2401 gtaaaatgcg ggttggccag gcgcgcgcgc tcgacgaaca attggatagc ttcaacctga
     2461 agcggatcat cctccacctc caggcgcagt ggcggcgcgg gccagatgac ttccccgccc
     2521 acccgcagcg cctcgcgact cgtcgcgagg atcgtcagcg acgcggcggc gcgcagcagc
     2581 acgtcggcca gcccggcgca tgtatcgatg agatgctcgc agttgtccag gacgatcaac
     2641 gaagggtcgc gcaagacgtc ggcgacatct tcgatcagcg agcggtctgc ttgaccagcg
     2701 acgccaagcc ttccggccac ctcggcgacg accagagcag ggtcggcgag gggggcgagg
     2761 tcgacgaacc agactccacc aacaaactcg tcagccagcc tgcgggcggc cgcgatcgac
     2821 aggcgcgtct tgccgctgcc accgggcccg gtcagtgtca ccagccgtga ccgccgcaac
     2881 aaattgcaaa tctcatcgag ctccggcacg cgcccaac
//
LOCUS       DASUGJ010000383         4758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1309336, whole genome shotgun sequence.
ACCESSION   DASUGJ010000383 DASUGJ010000000
VERSION     DASUGJ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309336"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VFV93_08450"
     CDS             complement(<1..1079)
                     /locus_tag="VFV93_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5165410.1"
                     /translation="MKHEHGRQPTLNDVARRANVSKQTISRVVNNKGEVSDATRQRVL
                     EAIREIGYHPNALARSLVTSRTHVIGLALPNIDQPFFPQIARGVEDAAAEAGYSVFLC
                     NASGDQERELNAIERLRGHRVAGVISFSSRLSDEAVERAIGGLFPAVMVNRELQGGRD
                     SVIWPGYETGSRLAVEHLLGLGRRRIVFLGLEQDSHVDSIKQAGYESALADAGVSLAP
                     ELIVRTSGKLGRSFNELAQGGQAAIAALLEQGVAFDAVFASNDLPAIGAMHHLAASGV
                     RIPDDIAVVGFGGANVAGLVTPTLSTVRIPLYEMGATAFHALLDRINHEHHGERLVNV
                     LPELIVRQSSGGPGQGRVGEERERVV"
     gene            1425..3386
                     /locus_tag="VFV93_08455"
     CDS             1425..3386
                     /locus_tag="VFV93_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5165411.1"
                     /translation="MTDERIQELIDAVRTRRLSRRAVLKRATALGLSAPVIAGLLAAC
                     GGDDDDEPEATATSATTGESTATSGSGGAATEEPSEEATEAGDGSPTSASAATEPSGA
                     EPTATTAEETGERGGGGILRILWWQAPVVLNGHLSVAGKDIGAIHICMEPLAQFGPDG
                     ELVPILAAEIPSLENGGVAEDQSSVTWKLREGVKWHDGEDFTAEDVVFTFTYLSDPLS
                     NATTLGFYSDVDTVEAVDDYTVTIHFKNPVAAWFNPFVGSSGTILPEHILRDFVGEQA
                     PHAPFNLMPIGTGPYKVSNFVPGDTVLYDIHQDYWAPGKPYFDQVELKGGGDAASAAR
                     AVLQSGEADWAWNLQVEAQILASLKDAGRGTLVTWPGAGTEKLLFNHSDPQTEKDGQK
                     SHRDVPHPHFQDLRVRQAVALAIPRDVIAEQLYGDAGEATGYTMNESPQFMPDGITWE
                     YNLDKANALLDEAGAAKGSDGIRELNGRKLDWIISASTNSVRQKEQEIIKQAFGELGI
                     QTEIAAVDASAYFDASNIASFQHLYYDFGIERNAAQIYPLLWYRRYLSADPLKDIAQA
                     ENGWAGRNIQRFQSPEFNEMYAKAAAETDPEVYTQIFLDMQTYVVENEADIGMVSGKN
                     VAAASSDLTGYKPSPFAVEVWDIRDWRKG"
     gene            3508..>4758
                     /locus_tag="VFV93_08460"
     CDS             3508..>4758
                     /locus_tag="VFV93_08460"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5165412.1"
                     /translation="MIVCPEPLAAAAGRDIFAAGGNAIDAAVGAAFVQGVTNPLLCGI
                     GGTGLFYYYDAASRQPVAMNGEVEIGSQPVPAHWPAEFRGRAETIGRYILESEANQVG
                     HQSVMTPGFVKVCWDAWRRYGSGKLSWAELLVPAIQLARGGYTIYPYIAAFWRPGVAS
                     DAHDARPGYPALMAKLHATAGAQEIYLKPDGGVFFDGDQIILSDYANTLQQLADAGGD
                     DFYSGEIARQAAADFAQHDGLFTATDLRDYVVIDQEILRSEYRGYEITSPAPPSSGAQ
                     VVEMLNILSHFDVSGLGHNSIDYIDLFARVQRASFSDNVRLKGIDFDDALEFASQVTN
                     PQIASYWAERIRNGDRIVVHGGAVDPGTTHVTVVDEQRNVVSFNHSIGSLAGSGVVTP
                     GLGFLWNNFLGHFNPLPGHTDSIVP"
BASE COUNT          848 a         1694 c         1500 g          716 t
ORIGIN      
        1 accacccgct cgcgctcttc gccgacgcgg ccctgtcctg gcccgccgga cgactggcga
       61 acgatgagtt ccggcagtac gttgaccagt cgctcaccgt ggtgctcgtg gttgatccgg
      121 tcgagcaggg cgtggaaggc ggtcgcgccc atctcgtaga gggggatccg gaccgtcgag
      181 agggtcggcg tcaccagccc ggcgacgttc gcgccgccga agccgacgac ggcgatatcg
      241 tcggggatgc ggaccccgct ggcggccagg tggtgcatcg cgccgatggc cggcaggtcg
      301 ttcgaggcga agacggcgtc gaacgcgacg ccctgttcca acagggccgc gatggccgcc
      361 tgcccgccct gggccagctc gttgaacgac cggccaagct tgccggacgt gcgaacgatc
      421 agctccggcg cgagcgacac cccggcatcg gccagcgccg actcatagcc ggcctgcttg
      481 atcgagtcga cgtggctgtc ctgctccagg ccgaggaaga cgatccgccg ccggccgagg
      541 ccgagcaggt gctcgacagc cagccgcgag cccgtctcgt agccgggcca gatgaccgag
      601 tcgcgcccgc cctgtagctc gcggttgacc atcacggccg ggaagaggcc accgatcgcc
      661 cgctcgaccg cttcgtcact gagccgcgag ctgaagctga tcaccccggc cacccggtgc
      721 ccgcgcagcc gctcgatcgc attcagctcc cgctcctggt cgccgctggc gttgcagagg
      781 aagacgctgt aaccagcctc ggcggcggca tcctcgaccc cacgggcgat ctgcgggaag
      841 aacggctggt cgatattcgg cagcgccagg ccgatcacat gcgtccggct cgtaaccaga
      901 ctgcgtgcca gtgcattagg gtggtagccg atctctcgaa tcgcctccag cacacgctgg
      961 cgggtagcgt ccgagacttc gcccttgttg ttgacgacgc gtgagatcgt ctgcttcgaa
     1021 acgttcgcgc gcctggcaac atcgttgagg gtcggttggc gaccatgctc gtgcttcata
     1081 gatgtagaca gtctttcttc aaattccacc tgctgacgga tagttcgcgt gatgggctaa
     1141 gtgtgcctca ttccgcgcgc cactccgcct accggccgat cgatgaaaaa ggagcgtacc
     1201 tgattgacca gtagccgaac tcgtgctagc gtgttaccgt tcccgtgaac gctaccggga
     1261 acggtatcac caccgtacag gtcagcggcg gcggcgtcaa gcacagcagt caccgccggt
     1321 cgtccatccc cggccggcca gaccatctgt cacccgcacc cgcaatcgtg acctgccgga
     1381 ccggtctgcc ggccattggt cgtagtctga gggggagaac cacgatgact gacgaacgaa
     1441 tccaggaact gatcgacgcg gtccggacgc ggcgcctgag ccgccgcgcc gtcctcaagc
     1501 gcgccaccgc gctcggcctg agcgcgccgg tcatcgccgg cctgctggct gcctgcggcg
     1561 gcgacgatga cgacgagccg gaggcaaccg caaccagcgc gacaaccggc gagtcaacgg
     1621 ccaccagcgg cagcggcggg gccgcgaccg aggaaccctc tgaagaggcg accgaagccg
     1681 gcgacggctc gccaaccagc gcgagcgcgg ccaccgagcc atccggcgcc gaaccgaccg
     1741 cgacgaccgc cgaggagacc ggcgagcggg gcggcggcgg catcctccgc attctctggt
     1801 ggcaggcgcc ggtcgtcctc aacggccatc tctcggtcgc cggcaaggat atcggcgcga
     1861 tccacatctg catggagccg ctggcccagt tcgggccgga tggcgagctg gtcccgatcc
     1921 tggcggccga gattcccagc ctcgagaacg gcggcgtcgc cgaggatcaa tccagcgtca
     1981 cctggaagct gcgcgagggt gtcaagtggc acgatggcga ggacttcacc gccgaggatg
     2041 tcgtcttcac cttcacctac ctgagcgatc cgctctcgaa cgcaacgacg ctcggcttct
     2101 acagcgacgt cgataccgtc gaggccgttg atgactacac cgtcacgatt cacttcaaga
     2161 atccggtcgc ggcctggttc aacccgttcg tcggcagctc cggcacgatc ctgcccgagc
     2221 acatcctgcg cgacttcgtc ggcgagcagg ctccgcacgc gccgttcaac ctgatgccga
     2281 tcgggaccgg cccctacaag gtcagcaact tcgtccccgg cgacaccgtc ctctacgaca
     2341 tccatcagga ctactgggcg ccgggcaagc cctacttcga tcaggtcgag ctcaagggtg
     2401 gcggcgacgc ggccagcgcg gcgcgggccg tcctccagtc cggcgaggcc gactgggcct
     2461 ggaacctgca ggtcgaagcg cagattctcg cctcgctcaa ggacgctggc cgcggcacac
     2521 tcgtcacctg gccgggcgcc gggaccgaga agctgctctt caaccactcc gacccgcaga
     2581 ccgagaagga cggccagaag tcgcaccgcg acgtgccgca cccgcacttc caggacctgc
     2641 gcgtccgtca ggccgtcgcg ctggcgatcc cgcgcgatgt catcgccgag cagctctacg
     2701 gcgacgccgg cgaggccacc ggctacacga tgaacgagag cccgcagttc atgccggacg
     2761 gcatcacctg ggaatacaac ctcgacaagg ccaacgcgct gctcgacgaa gccggcgcgg
     2821 ccaagggcag cgacggcatc cgcgagctga acggccgcaa gctggactgg atcatctccg
     2881 cctccaccaa cagcgtccgc cagaaagagc aggagatcat caagcaggcg ttcggcgagc
     2941 tgggcatcca gaccgagatc gccgctgttg acgccagcgc ctacttcgac gcgtccaaca
     3001 tcgcgtcgtt ccagcacctg tactacgact tcggcatcga gcgcaacgcg gcccagatct
     3061 acccgctgct ctggtaccgg cgctacctct cggctgaccc gctcaaggac atcgcccagg
     3121 ccgagaacgg ctgggccggc cgcaacatcc agcgcttcca gagccccgag ttcaatgaga
     3181 tgtacgcgaa ggcggccgcc gaaaccgacc ctgaggtcta cacccagatc ttcctcgaca
     3241 tgcagaccta tgtcgtcgag aacgaggccg acatcgggat ggtctccggc aagaacgtcg
     3301 ccgccgccag cagcgacctg accggctaca agccctcccc cttcgccgtc gaagtctggg
     3361 atatccgcga ttggcggaag gggtaggaga tgaggagatg aggggatggg gcatgaggga
     3421 tgagtgacgt gatcgttcct ggtctcatcc ctcatgcccc atcccccgtc cccaccacac
     3481 acgaaaggat gcacgtcgtt gactaacatg atcgtctgcc ccgagccgct ggcggcagcg
     3541 gcggggcggg atatcttcgc ggccggtggg aacgccatcg acgcggccgt cggggcggcc
     3601 ttcgttcagg gcgtcaccaa cccgctcctg tgcgggatcg gcgggacggg gctgttctac
     3661 tactacgacg ccgccagccg gcagccggtc gcgatgaacg gcgaggtcga gatcgggtcg
     3721 caaccggtcc cggcccactg gccggccgag ttccgtggcc gggctgagac gatcggccgc
     3781 tacattctgg agagtgaggc caaccaggtc ggccaccagt cggtcatgac gcccggcttc
     3841 gtcaaggtct gctgggacgc ctggcgacgc tacggcagcg gcaagctctc gtgggcggaa
     3901 ctgctggtgc cggcgatcca gctcgcacgg ggcggctaca cgatctaccc ctacatcgcc
     3961 gccttctggc gtcccggcgt cgccagcgac gcccacgacg cccggcccgg ctacccggcc
     4021 ctgatggcca agctccacgc gacggccggc gcacaggaga tctacctcaa gccggatggc
     4081 ggcgtcttct tcgacggcga ccagatcatc ctcagcgact acgccaacac cctccagcaa
     4141 ctggccgacg ccggtggcga cgatttctac agcggcgaga tcgcccgtca agctgccgcc
     4201 gacttcgcgc agcacgacgg actcttcaca gccaccgacc tgcgcgacta tgtcgtcatc
     4261 gatcaggaga tcctgcgttc cgagtatcgc ggctacgaaa tcaccagccc ggcgccacca
     4321 tccagcggcg cacaggtcgt cgagatgctc aacatcctca gccacttcga tgtcagcggg
     4381 ctgggccaca acagcatcga ctacatcgac ctcttcgccc gcgtccagcg cgccagcttc
     4441 agcgacaacg tccggctcaa ggggatcgac ttcgacgacg cgctggagtt cgccagccag
     4501 gtcaccaacc cgcagatcgc cagctactgg gccgaacgga tcaggaacgg cgaccggatc
     4561 gtcgtccacg gcggcgcagt cgatcccggc acgacccacg tcaccgtcgt tgacgagcag
     4621 cgcaacgtcg tctcgttcaa ccactcgatc ggctcgctgg ccggctccgg cgtcgtcacg
     4681 cccggcctcg gcttcctctg gaacaacttt ctgggccact tcaacccgct gcccggccac
     4741 accgactcga tcgtgccc
//
LOCUS       DASUGJ010000384         4944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1727691, whole genome shotgun sequence.
ACCESSION   DASUGJ010000384 DASUGJ010000000
VERSION     DASUGJ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727691"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1164)
                     /locus_tag="VFV93_08465"
     CDS             complement(40..1164)
                     /locus_tag="VFV93_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165413.1"
                     /translation="MVIESVRLSRSELQQRQVVDLERIVADLPDDHRRTFERLYHVSP
                     TIGRLNPPEHMRRWIDKYFGSVDSVAEQKVVKVTNRWTLEGSLFNELRARRPLEARIP
                     ADLAEEIARTAPDPFCEPELNTPEDVFGRIRGRHSITASNIAKYDGFHGVVIFEEHDP
                     LAFTEDSVQDAIDVSKQWFQKCHEIDDSAIYPLVMWNCLWKSGASIPHGHMQVSLTRG
                     MHYGQIERQRRVAANYRHEHGRDYHDDVFAVHEALGLGFHHRGVRVLASITPRKEKEC
                     VLLGTALDETFVSALYDVLNTLTDELNVVSFNLVVWHPPLEDTEEDWSDFPIIARMVD
                     RGDPLNRTADFGAMELYAASVVSSDPFKVAGALRDSLGVD"
     gene            complement(1497..2786)
                     /locus_tag="VFV93_08470"
     CDS             complement(1497..2786)
                     /locus_tag="VFV93_08470"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX5165414.1"
                     /translation="MAAEREKTVAMILAGGQGERLSVLSRQRAKPAVMFGGKYRIIDF
                     ALSNCVNSHIYDVAVLTQYRPHSLNDHIGHGRSWDLDRANGGVRVLQPYLGRARSDWY
                     RGTADAIYHNLFYVEETRATDVLILSGDHVYAMDYRPMLQLHRETGADVTVAVQPVPW
                     EDASRFGVMSVNEDGLVVDFEEKPEKPRSNLASMGVYVFKADVLHEVFRREDAGRTMI
                     DFGHEVIPYLIGDDNVHAYRFLNYWRDVGTLQSYWEANMEQLQDVPGLNLYDPDWRIH
                     TRSEERPPAKVMDGSQVSRAMISNGCIIIRGSVTHSVLSPGVTIHEGAVVRDSIIMTD
                     AVIGPYAQIDRCIIDKQVWIGPGTKLGVGDDFSPNWLEPTRINTGLTIVGRNAHVPAG
                     VTAGRNVLIGADVKESDFESLEIVSGETVDPRVPIWA"
     gene            2984..3922
                     /gene="accD"
                     /locus_tag="VFV93_08475"
     CDS             2984..3922
                     /gene="accD"
                     /locus_tag="VFV93_08475"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX5165415.1"
                     /translation="MRELFRRQPKFTPTGEDDLRSHVPDDLWVKCPKCGELVYTKEHM
                     RSLRVCQRCGYHFRLSASERIEMLADEDTFVEWDAEVTTSDPLGFVVDDEGYLVKAQK
                     TATRSGVGEALIAGKLKLDGKPVAVVVTDFSFMGASMGSVFGEKLARACERAVDEGLP
                     LLTVSASGGARMQEGLFSLMQMAKTTAALTRLGDARLPHISLLTDPCYGGVTASYATV
                     ADVILAEPAALIGFAGPRVIEQITRQKLPAGFQTAEFLLEHGMIDQVTPRHSMIKTLS
                     ALFDLYGGLRTGRRTRRRRIDAAEPDAVTTGAADGE"
     gene            3912..4787
                     /locus_tag="VFV93_08480"
     CDS             3912..4787
                     /locus_tag="VFV93_08480"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX5165416.1"
                     /translation="MASSTSSNGIGPWERVLLARHANRPHTLAYIGGLCDEFVELHGD
                     RAAGDDAAIVGGIGRFRGRTVMLVGHQKGESTRENIQRNFGMPKPEGYRKALRLMQQA
                     EKFGMPLISFIDTPGADPGLESEEHGQAFAIAANVFAMADLTIPTVALVIGEGGSGGA
                     LAIGVADRVLMLENAIYSVASPEACAAIIWKDAGRAPDAAAAMRVNAVDLLQFGLIDE
                     LVPENPAAHEDPQATITASGDAIERHLIELEETWKRGAAGERAMLEARYAKYRAMGEW
                     HEAQAVPTPAGIGRR"
BASE COUNT          877 a         1598 c         1600 g          869 t
ORIGIN      
        1 ccatcgcccc tacacgaagt gactctggcg aaatcggatc taatccacac ccagcgaatc
       61 gcgcagcgcc cccgccacct tgaaagggtc gctggagacg acgctggcgg cgtagagctc
      121 catcgcgccg aagtcggcgg tgcggttgag cgggtcgccg cgatcgacca tccgggcgat
      181 gatcgggaag tcggaccagt cctcctcggt gtcctcaagc ggcgggtgcc agacgaccag
      241 gttgaaagag acgacgttga gctcgtcggt cagcgtgttg agcacgtcat acagcgcgct
      301 gacgaacgtc tcatccagcg ccgtcccgag caggacgcac tccttctcct tgcgcggcgt
      361 gatgctggcc agcacccgga cgccccggtg gtggaaacca agcccgagcg cctcgtggac
      421 ggcgaagaca tcgtcgtggt aatcgcggcc gtgctcgtgg cggtagttgg cagccacccg
      481 gcgctggcgc tcgatctgac cgtagtgcat gccacgcgtc aggctgacct gcatgtggcc
      541 gtgcgggatc gacgcgccgc tcttccagag gcagttccac atcaccagcg gatagatcgc
      601 ggaatcgtcg atctcgtgac acttctggaa ccactgcttc gagacatcaa tcgcgtcctg
      661 gaccgagtct tcggtgaagg cgagcgggtc gtgctcctcg aagatgacga ctccgtggaa
      721 gccgtcgtac ttggcgatgt tgctggcggt gatggagtgc cggccacgaa tgcggccgaa
      781 gacgtcctcg ggcgtgttca gctcgggctc gcagaacggg tccggtgccg tccgggcgat
      841 ctcctcggcc aggtcggccg ggatgcgcgc ttcgagggga cgacgggcac gcagttcgtt
      901 gaagagcgac ccttcaagcg tccagcggtt ggtgaccttg acgaccttct gctcggcgac
      961 gctgtcgacg ctgccgaagt acttgtcgat ccagcgccgc atgtgttcgg gcgggttcaa
     1021 gcgaccgatg gttggactga cgtgatagag acgctcaaag gtgcgccggt ggtcatccgg
     1081 aaggtcggca acgatccgct cgaggtcgac aacctgacgc tgctgtaatt cagatcggct
     1141 aagacgcact gattcgatca ccatttccac tccttgactg cggtgcgggc ggcatcgtaa
     1201 tggcgtcgcc caacgacggt acaccgcgag tcagtacctg cgtctgaacg cagccccttt
     1261 acagcatgat tgagcgtcca tgtagttgag atctcgtgac tggtcgtcaa tctgaagaga
     1321 atgatgacac tcctacccgg ttgcggtttg gccccggctg gcctacgtca tccatctgca
     1381 tcaagcctat caggtgccaa cgaggcaccg aaatagcccg ccagtcgcgg tttgcacttc
     1441 tcaccgcagc cggttccact ctatcatatg tacgtacacc ttgtcgcgat ccgattctac
     1501 gcccagatcg gcacgcgggg atcgactgtt tccccgctga cgatctccag tgactcgaaa
     1561 tccgactcct taacatcggc gccgatcagg acattgcgac cggccgtgac gccggccggc
     1621 acgtgggcat tacggccgac gatcgtcagg ccggtgttga tccgggtcgg ctcaagccag
     1681 ttgggcgaga agtcgtcgcc gacgcccagt ttcgtgcctg gcccgatcca gacctgcttg
     1741 tcgatgatgc agcggtcgat ctgggcgtat gggccgataa cggcgtcggt catgatgatc
     1801 gagtcgcgaa cgaccgctcc ctcgtggatc gtgacacccg gcgagagaac cgagtgcgtc
     1861 accgagccac gaatgatgat gcagccgttg gagatcatcg cccgtgagac ctgcgagccg
     1921 tccatgacct tggccggcgg gcgctcctcg ctgcgggtgt ggatgcgcca gtccggatcg
     1981 tagaggttca gccccggcac gtcctgcagc tgctccatgt tggcttccca gtacgactgg
     2041 agcgttccga catcgcgcca gtagttcagg aagcgatagg cgtggacgtt gtcgtcgccg
     2101 atcaggtatg ggatgacctc gtggccgaag tcgatcatcg tccggccggc atcctcgcgg
     2161 cggaagactt cgtgcaggac atcggccttg aagacgtaga cacccatcga ggccaggttg
     2221 ctgcgcggtt tctccggctt ctcctcgaag tcgacaacca gcccgtcctc gttgaccgac
     2281 atgacgccga agcggctggc gtcctcccac ggcaccggct ggacggcaac cgtcacgtcg
     2341 gcgccggtct cgcggtgcag ttgcagcatt ggccggtagt ccatcgcgta gacgtgatcg
     2401 ccggagagaa tcagcacatc ggtcgcgcga gtctcctcga catagaacag gttatggtaa
     2461 atcgcatcgg ctgttccgcg ataccagtcc gaccgcgccc ggccgagata cggctggagc
     2521 acgcgaacac cgccgtttgc ccggtcgaga tcccaggatc ggccatggcc gatgtggtcg
     2581 ttgagtgagt ggggacggta ctgcgtcagg acggcgacat cgtagatgtg ggaattgacg
     2641 cagttagaga gcgcgaagtc gatgatgcgg tacttgccgc caaacatgac ggccggcttg
     2701 gcgcgttggc gcgacaggac acttagacgc tccccttgcc caccggccag gatcatcgcg
     2761 actgtcttct cgcgctccgc cgccattggt cgtccttccg tgccctgaat caccggcccc
     2821 gcgtcaccgg ctctcaagac gcaagataca tgctgtcgga atccggtcaa gcaggctgat
     2881 gcgctatgct atactgcgcc gctgtcgccg cgccggcatt gcatggtggg atgcagtcga
     2941 tatcgccggg gcgcggcccg cgctggacgg caccaggaca tcgatgagag aacttttccg
     3001 acgccagccg aagtttacgc cgacgggcga ggatgacctg cgttcgcatg tacccgacga
     3061 cctgtgggtc aagtgcccca agtgtggcga gctggtctac accaaggagc acatgcggag
     3121 cctgcgcgtc tgccagcgct gcggctacca cttccggctg tccgccagcg agcgcatcga
     3181 gatgctggcc gacgaggaca cgttcgtcga gtgggacgcc gaagtgacga cgtccgaccc
     3241 gctcggcttc gtcgtcgatg atgagggcta tctcgtcaag gcgcagaaga cggccacccg
     3301 ctccggtgtc ggcgaggcgc tgatcgccgg caagctgaag ctggatggca agcccgtcgc
     3361 cgtcgtcgtg actgacttct ccttcatggg cgccagcatg ggctcggtct tcggcgagaa
     3421 gctggcccgt gcctgcgaac gggccgttga cgaagggctg ccgctgctga ccgtctcggc
     3481 gtccggcggc gcgcggatgc aggaggggct cttctcgctg atgcagatgg ccaagacaac
     3541 ggcagccctg acgcgtctcg gcgacgcccg tctgccgcat atctccctgc tgaccgatcc
     3601 gtgctatggc ggcgtcaccg ccagctacgc gaccgtcgcc gacgtgatcc tggccgaacc
     3661 ggccgcgctg atcggcttcg ccggcccgcg cgtcatcgag cagatcaccc gccagaagct
     3721 gccggccggc ttccagacgg ccgagttcct gctggagcac ggcatgatcg atcaggtcac
     3781 accgcgtcac agcatgatca agacgctctc ggcacttttc gacctgtatg gcggcttgcg
     3841 aaccggccgg cgtacccgtc gccgccggat cgacgccgcc gagccggatg ccgtgacgac
     3901 aggagcagcc gatggcgagt agcacctcga gcaacggcat cggcccctgg gagcgcgtcc
     3961 tgctggcgcg gcatgccaac cgcccgcaca cgctggccta catcggcggc ctctgcgacg
     4021 agttcgtcga gctgcacggc gaccgggccg ccggtgacga cgccgcgatc gtcggcggaa
     4081 tcggccgctt tcgtggccgg acggtgatgc tggtcggtca ccagaagggt gaatcgaccc
     4141 gcgagaatat ccagcgcaac ttcgggatgc cgaagccgga gggctaccgc aaggcgttgc
     4201 ggctgatgca gcaggccgag aagttcggga tgccgctgat cagcttcatc gacacgcccg
     4261 gcgccgatcc cggccttgag tccgaagagc acgggcaggc gttcgcgatc gccgccaacg
     4321 tcttcgcgat ggccgacctg acgatcccaa ccgtcgcgct cgtcatcggc gaaggcggca
     4381 gcggtggcgc gctggcgatc ggcgtcgccg accgcgtcct gatgctcgag aacgccatct
     4441 actcggtggc ctcgccggaa gcctgcgccg ccatcatctg gaaggacgcc ggccgtgcgc
     4501 cggatgccgc cgctgcgatg cgcgtcaacg ccgtcgatct gctccagttc ggcctgatcg
     4561 acgaactcgt ccccgagaac ccggccgccc acgaggatcc acaggcgacg atcaccgcct
     4621 ccggcgatgc gatcgagcgc cacctgatcg agttggaaga gacctggaag cggggcgcag
     4681 ccggcgagcg cgccatgctt gaagcgcgct acgccaagta ccgcgcgatg ggcgaatggc
     4741 acgaagccca ggctgtaccg actccagccg gaatcggccg ccggtaacca gcaacctcac
     4801 gcgacacgcg aaaattgggg tttgacaccg tcgcggcgcc tgcctatagt acgtcaggtt
     4861 gttccagcac gaacttcaac actgccagaa ccgacaacct cgggcatgag cgttgacccg
     4921 gttgttgcga ccggataggt cgcc
//
LOCUS       DASUGJ010000385         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_754244, whole genome shotgun sequence.
ACCESSION   DASUGJ010000385 DASUGJ010000000
VERSION     DASUGJ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754244"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFV93_08485"
     CDS             <1..451
                     /locus_tag="VFV93_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165417.1"
                     /translation="LTEHGTLNQRLVREPWGWAVLWAAAFTFAWLAFSGKRFGKALRP
                     PPSWARRSSGEYVTTLGTLLRRGKHERWLRDHYAAQVKRAIGSRYRIRADQPAREFAS
                     ALSERRADAADLAAPLERLESPQPLDGATTLALMRDVDDLKRRMGGA"
     gene            497..1447
                     /locus_tag="VFV93_08490"
     CDS             497..1447
                     /locus_tag="VFV93_08490"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5165418.1"
                     /translation="MSELTTIRERFLREAERAVVGQDETLELLLVSLLAGGHVLLEGV
                     PGTAKTLMAKTTAALLRAEFSRVQFTPDLMPSDILGTSVFDFQTGHFNLRRGPIFTNI
                     LLADEINRAPAKTQSALLEAMEERQVTLEGDRHRLPDLFMVVATQNPIEYEGTYPLPE
                     AQLDRFFFKLLVDHPDAEVEQAILARYHHGFDARQLDALQLEPVIDEPTLASLRAGWQ
                     AVAVEPGVMTYITSIIRATRASADVLLGASPRAGIALLLGSKVLAALSGREYVLPDDV
                     KRLALPVLRHRILLKPEAELEALTPDQVIQRLLAGVEVPR"
     gene            1448..2782
                     /locus_tag="VFV93_08495"
     CDS             1448..2782
                     /locus_tag="VFV93_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5165419.1"
                     /translation="MSLSFVPARRLLWAGLTTIIPLVLATWVPGLEWVALAWMLGLAG
                     VVVLDAYRSPRAGQFEVERRHDPRLSLAEANPVDIEIRWNLPGMTGRPRQLYVRDEAP
                     VEIPNDGTLFDGTITPGGSWTGRYHLSPRRRGDYPFGAVVLRVASPLGLLFWQRRYEL
                     EDAARVYPNLRAIARYDLLARRGRLEEAGLRRTRRFGSGTEFERLRDYLPDDDYRRIN
                     WKATARRRQPVTVEYETERSQNLVLVLDTGRLMGTPVGRMQKLDYAVNAALMLAYVAM
                     EMGDRTALLIFTDRTEGFVPLGRGKRQFQLLLDTLYKVETHPIEADPTRALNDLLRRQ
                     LRRSLIVVFTDVAESVEPTPLLLSLGQLARRHLPLCVMVSNPDITRLAVAPPSTSQAA
                     YEKVIAQRLIDERRGFTDRLERRGTLVLDAPAEQLTTDLINRYLEIKAKTKL"
BASE COUNT          443 a          984 c          966 g          462 t
ORIGIN      
        1 cttgaccgag catggcacgc tcaaccagcg actggtgcgg gagccgtggg gctgggccgt
       61 cctctgggcg gcggcgttca cctttgcctg gctggcgttc tctggcaagc gcttcggcaa
      121 ggcgttgcga ccgccaccaa gctgggcgcg ccggtcgagc ggcgagtacg tcacgacgct
      181 cggcacgctg ctgcgacgcg ggaagcacga gcgctggctg cgtgatcatt acgctgccca
      241 ggtaaagcgg gcgatcggca gccgctaccg gattcgcgcc gaccaaccgg cccgcgagtt
      301 cgcctccgcc ctcagcgaac gccgcgccga cgcagccgat ctggccgcgc cactcgaacg
      361 cctcgaatcg ccccagccgc tcgacggggc aacgacgctg gcgctgatgc gcgatgtgga
      421 cgatctgaaa cgccggatgg gtggggcgtg atgcggggcg cgggacgcga ctatgtgcgg
      481 tgataggggt tgacgattga gcgagctgac aaccatccgt gaacggttcc tgcgcgaggc
      541 ggagcgggct gttgtcgggc aggatgagac gctggaactg ctgctggtca gcctgctggc
      601 gggcgggcat gtcctgctgg agggtgtgcc aggcacggcc aagacgctga tggcgaagac
      661 gacagcggcc ttgttacgtg cggagttctc acgcgtgcag ttcacgcccg acctgatgcc
      721 gtccgacatc ctcggcacga gcgtcttcga tttccagacc gggcacttca acctgcggcg
      781 ggggccgatc ttcaccaaca tcctgctggc cgacgagatc aaccgcgcgc cggccaagac
      841 gcaatccgcc ctgctggagg cgatggagga acgccaggtc acactggagg gtgaccgcca
      901 ccggcttccg gatctgttca tggtcgtcgc gacccagaac ccgatcgagt acgagggcac
      961 ctatccgctg ccggaagccc aactggaccg cttcttcttc aagctgctgg tcgatcatcc
     1021 cgacgccgag gtcgagcagg cgatcctggc ccgctaccac cacgggttcg acgcccgcca
     1081 gctcgacgcg ctccagcttg aaccggtcat cgacgagccg acactggcga gcctgcgggc
     1141 cggctggcag gcggtcgccg tcgagcccgg cgtcatgacg tacatcacca gcatcatccg
     1201 cgcgacgcgg gcctctgccg acgtcttgct gggcgccagt ccccgggccg ggatcgcgct
     1261 gttgctgggt agcaaggtgc tggcggcgct gtctggccgt gagtacgtcc tgccggacga
     1321 tgtcaagcgg ctggcgctac cggtcctgcg ccaccggatt ctgctcaagc cggaggccga
     1381 gctggaggcg ctgacacccg accaggtcat ccagcgcctg ctggccggcg tcgaggtgcc
     1441 gcgctagatg tcgctctcgt tcgtgcctgc ccgcaggctg ctctgggccg ggctgacgac
     1501 catcatcccg ctcgtgctgg cgacctgggt tcccggcctg gagtgggtcg cgctcgcctg
     1561 gatgcttggg ctggccggcg tcgtcgtgct cgacgcctat cggtcgccac gggccggcca
     1621 gttcgaggtc gagcggcggc acgacccgcg cttgtcgctg gccgaggcta acccggtcga
     1681 catcgagatc cgctggaacc tgccggggat gacgggccgc ccgcgccagt tgtacgtccg
     1741 cgacgaagcg ccggtcgaga tccccaacga cggcacgctg ttcgacggca cgatcacgcc
     1801 cggcggaagc tggactggcc gctaccatct ctcgccgcgc cgccgtggtg actacccgtt
     1861 tggcgcggtc gtcctgcgcg tggcctcgcc gctcgggctg ctcttctggc agcggcgcta
     1921 cgaactggag gatgccgccc gtgtctaccc caacctgcgc gcgatcgccc gctacgacct
     1981 gctggcgcgt cgggggcgac tggaggaggc ggggctacgg cggacgcggc gcttcggcag
     2041 cgggaccgag ttcgagcggc tacgtgacta cctgcccgac gacgactatc gtcgcatcaa
     2101 ctggaaggcg accgcgcgac gccgccagcc ggtgacggtc gagtacgaga cggagcgcag
     2161 ccagaacctc gtcctcgtcc tcgacaccgg ccggctgatg ggaacgccgg tcggcaggat
     2221 gcagaagctc gactacgccg tcaacgccgc cctgatgctg gcctacgtcg caatggagat
     2281 gggggacagg acggccttgc tgatcttcac cgaccggacc gaaggctttg ttccgctcgg
     2341 gcggggcaag cgccagttcc agctcttgct cgacacgctc tacaaggtcg agacccatcc
     2401 gatcgaggct gacccgaccc gcgcgctgaa cgacctgctc cgtcgtcaac tgcgtcgctc
     2461 gctgatcgtc gtcttcaccg atgtcgccga gtcggtcgag ccgacgccgc tgctgctctc
     2521 gctcggccag cttgcccgcc gccacctgcc actctgcgtg atggtcagca acccagacat
     2581 cacccgcctg gcagtcgctc cgcccagcac cagccaggcc gcctacgaga aggtcatcgc
     2641 ccagcgattg atcgacgagc gccgcgggtt taccgaccgc ctcgaacgcc gcggcaccct
     2701 cgtcctcgac gcccccgccg aacagctcac gacggatttg atcaacaggt atctggagat
     2761 caaggcgaag acgaagctgt gacagggtcg tgacgcgtga cgcgtgacgc ggtttttggg
     2821 cggattcctc gcgtcacgca tcacgcgtta cgcgt
//
LOCUS       DASUGJ010000386         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1171243, whole genome shotgun sequence.
ACCESSION   DASUGJ010000386 DASUGJ010000000
VERSION     DASUGJ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171243"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFV93_08500"
     CDS             <1..811
                     /locus_tag="VFV93_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165420.1"
                     /translation="EGATVDKDGRMILPEGRRQARNAEFGLPGRSKLLSYVPGRGVDQ
                     HRDDVGKRIPAGKYINWQMHYNPTGKPEKDRSRLGIWFNKVPVKYEVLIRQAGDPLAT
                     TKGGLSLYRAEGKEVEYTADEGSQQRNRRTPNIPPYAENWSLTGITPVTEDITLYAMS
                     PHMHLRGKSLKWVITYPDGREQTILNVPKFDFNWQINYELAEPLHIPAGSKIAGIGFY
                     DNSPKNKWNPGPQLEVYWSEQSWDEMYQPFTEYSIDSQDLTKIVPVTQQQQ"
     gene            864..939
                     /locus_tag="VFV93_08505"
     tRNA            864..939
                     /locus_tag="VFV93_08505"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Thr,seq:cgt)
     gene            1030..2019
                     /locus_tag="VFV93_08510"
     CDS             1030..2019
                     /locus_tag="VFV93_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5165421.1"
                     /translation="MLTILLAASLGVPAAGASRLDQIRARGIVTCGVGPGVPGFSEVD
                     AQGRYSGFDIDICRALSAAIFGQPDRVRYVITPTIQAFLRSSDIDVISRRLTLTLTRE
                     GLGVLFGPIVFYDGQGFLVSKTIGAKRIADLAGATACVQAGSEHDDKLTSYMSVNKLA
                     FRRVPVADLAQARNSLTAGRCQSLSDDVSALAGVRAALPAPDDFEILPELMTKEPLAQ
                     LVRNDDVQFFSILRWTIFALIEAEETGVNSTNVDQMLKSSNPRIQALLGVAGNTGSAL
                     GLDAKWARNIIKSLGHYGEIYERNIGARSRIKLARGLNRLWTEGGLIYAPPLQ"
BASE COUNT          412 a          641 c          639 g          335 t
ORIGIN      
        1 cgaaggcgcc acggtggaca aagacggccg catgattctg cccgaaggca ggcgccaggc
       61 ccgcaacgct gaattcggac tgccgggacg ctcgaagctg ctgtcgtacg tgcccggtcg
      121 cggcgtcgac cagcaccgcg acgacgtcgg caagcggatt cccgccggca agtacatcaa
      181 ctggcagatg cactacaacc cgacgggcaa accggaaaag gatcgctcgc ggctcggcat
      241 ctggttcaac aaggtcccgg tgaagtacga agtgctgatc cgccaggccg gcgatccgct
      301 ggccacgacc aagggcgggc tctcgcttta tcgcgcggaa ggcaaggaag tcgagtacac
      361 ggccgacgaa ggcagccagc agcgcaaccg ccggacgccg aacattccgc cctacgccga
      421 gaactggagc ctcaccggca tcacgccggt gactgaggac atcacgctct atgcgatgtc
      481 accgcacatg cacctgcgag gcaagagcct gaagtgggtg atcacctatc ccgacggccg
      541 cgagcagacg atcctcaacg tgccgaagtt cgacttcaac tggcagatca actacgagct
      601 ggccgagccg ctgcacatcc cggccggcag caagatcgcg ggcatcggct tctacgacaa
      661 ctcgccgaag aacaagtgga atcccggacc gcagctcgag gtgtactggt ccgagcagag
      721 ctgggacgag atgtatcagc cgttcaccga gtactcgatc gacagccagg acctgacgaa
      781 gatcgtgccg gtgacgcagc agcaacagta atcctgaagt cgcgacgcgc gcctgctgtg
      841 ctacgatcgg aggtcctctc agggccggcg tagctcagtt ggtagagcag tcgattcgta
      901 atcgacaggt cactggttca agtccagccg ccggctccaa ccttcgctca tgaacgcagg
      961 cgaaggttgc ccgccgtagg tcgtcctgag atcttcgagc gaaggcgggc tgtcttgcga
     1021 atccgaacga tgctgaccat tctccttgcc gccagcctcg gcgtgcctgc ggccggcgcg
     1081 tcgaggctcg atcagatccg cgcccgcggg atcgtcacct gcggcgtcgg gcccggcgtt
     1141 ccaggtttct cggaagtcga tgcccagggt cggtactcgg gcttcgacat cgacatctgc
     1201 cgcgcgctct cggcggcgat cttcggccag ccggatcgcg tccgatacgt cattacgccg
     1261 acgattcagg ctttcctgcg ctcatcagac atcgacgtca tctcccggcg cctgacgctg
     1321 acgctgacgc gtgagggact cggcgtgctc ttcggcccga tcgtgtttta cgacggacag
     1381 gggtttctgg tctcgaagac gatcggcgct aaacggattg ccgatcttgc gggggcaaca
     1441 gcgtgcgtcc aggccggctc cgaacacgac gacaagctca cgtcttacat gagcgtcaac
     1501 aagctggcgt tcagaagagt gccggtcgcc gatctggccc aggcgcgcaa ctcactgacg
     1561 gccggccgct gccagtcgct ttcagacgac gtctctgcgc ttgcgggagt gcgagcggcg
     1621 ttaccggcgc ccgacgactt cgagatactg ccggaactca tgacgaaaga gccgctggcg
     1681 caactggtcc ggaacgatga cgtgcagttc ttcagcattc tgcgatggac gatcttcgcc
     1741 ctgatcgaag ccgaggagac cggggtcaat tcgacaaacg tcgatcagat gctgaaaagc
     1801 agcaacccca ggattcaggc gttgctgggc gtggcgggaa ataccggaag cgcgctcggg
     1861 ctcgacgcga agtgggcgcg caacatcatc aagtcgctgg gacactacgg cgagatttac
     1921 gagcggaaca tcggtgcgcg aagccggatc aagctcgcgc gcggcctgaa ccggctgtgg
     1981 accgagggcg gtcttattta cgcaccgccg ctccaatagc aaccagc
//
LOCUS       DASUGJ010000387         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1309758, whole genome shotgun sequence.
ACCESSION   DASUGJ010000387 DASUGJ010000000
VERSION     DASUGJ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309758"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFV93_08515"
     CDS             complement(<1..379)
                     /locus_tag="VFV93_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5165422.1"
                     /translation="MTHHEPTGLIGQPIKREDDRKLVSGRGRFVDDLNPPGTLHVAFV
                     RSPYAHAEIVSVETAAAKNAPGVVAVVTGAGLGEWMKPTPIVEALLPNRPLIRHSLVA
                     DRARYAGDAVAAVIAETAAQAADA"
     gene            complement(558..1667)
                     /locus_tag="VFV93_08520"
     CDS             complement(558..1667)
                     /locus_tag="VFV93_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5165423.1"
                     /translation="MTAARVAAIVLAAGSSSRLGRPKQLLPVGQTPLLQCTLNMVRQT
                     TLEPRILVLGGYADDILRAVDPRGFDVVHNPDYASGQASSLRAGLTALPAGIDAAAVI
                     LGDQPLVPPWLLDELASAFEPQQQVAVRPEFSDGPGNPVILGRELFPALLALEGDVGA
                     RDILRANRDRVLALPVPNRMTPRDVDTMADFEALLLDWSSTGAPDVPRYCQRCGAEVG
                     FKVIHQRLRPACPVCGFTYYYDPKVATAVVVEIDGKVVLQQRAIDPGIGKWTFPGGFV
                     ERGEPVREAAVREILEEVGVTVAAEELTLTGVYSEPGETVILVAWYVRADGQTPRIAD
                     RESTAVQLFAPDALPDLAFPRNVRVLADARRLTGR"
     gene            complement(1664..>2364)
                     /locus_tag="VFV93_08525"
     CDS             complement(1664..>2364)
                     /locus_tag="VFV93_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX5165424.1"
                     /translation="AKVVSGPDTGSELLIVYPDQVLGGLGSPAADEQASRTLRAMLRD
                     GGTTLDEIDGRQVFMEAHLPPPHLVIVGAVHIAIPLVTFGQTLGYRTTVIDARAQFAT
                     DERFPHADRLIEAWPDEALAQLNLHPGVAAVCLAHDPKFEDPAMEVLLRSDAGYIGAV
                     GSRTTSMERRERLKGAGFTDKQLDRIHGPVGLNIGGKTPEEIALSIMAEIVAVRRGRD
                     PRAAVAPAKATTGS"
BASE COUNT          381 a          738 c          878 g          367 t
ORIGIN      
        1 cagcgtcggc agcctgggca gccgtctcgg caatgacggc cgcgacggcg tctccggcat
       61 agcgggcgcg gtctgcgacg agggagtgcc ggatgagcgg gcggttcggc aacagcgcct
      121 cgacgatcgg cgttggcttc atccactcgc caagcccggc gccagtgacg acagcgacga
      181 cgccgggggc gttcttcgca gccgctgtct cgaccgagac gatctcggcg tgggcgtagg
      241 gcgagcgaac gaaggcgacg tgcagcgtgc cgggcgggtt gagatcgtcg acgaagcggc
      301 cccgtccact gaccagcttg cggtcgtcct cgcgcttgat gggttgaccg atcaggccgg
      361 ttggttcgtg gtgagtcacg gtttcgggtt cctctcgtac atatcgagcc gtcaagaacc
      421 cctccccaat cgccacccag agggtccccg gcctgagaag ggcggggctt tgcgcctaac
      481 gaaatctaag ccccctctcc ccacagggga gggggttgcg ggaggggttt ctccccgtcc
      541 gcgagtttcg ccgttcctca gcgcccggtc aagcggcggg cgtcggcgag gacgcggacg
      601 ttgcggggaa acgcgagatc ggggagggca tcaggcgcga agagctggac ggcagtgctc
      661 tcgcgatctg cgatccgcgg agtctggccg tcagcacgga cgtaccaggc gacgaggatg
      721 acggtttcgc cgggctcgct gtagacgccg gtcagcgtca gttcttccgc tgcgacggtg
      781 acgccgacct cttcgagaat ctcgcgaaca gccgcctcgc ggaccggctc gccccgctcg
      841 acgaagccgc cggggaaggt ccacttaccg atgcctgggt cgatcgcccg ctgctggagg
      901 acgaccttcc cgtcgatctc aacgacgaca gcggtcgcga ccttggggtc gtagtagtag
      961 gtgaagccgc agaccgggca ggcggggcga agacgctggt gaatcacctt gaagccgact
     1021 tcggccccgc agcgttggca gtagcgcggg acatccggcg caccggtcga tgaccagtcg
     1081 agcagcagcg cctcgaagtc ggccatcgta tcgacatcgc gtggggtcat gcggttcggg
     1141 accggtagcg ccaggacacg atcgcggttg gcgcggagga tgtctcgcgc gccgacgtca
     1201 ccttcgagcg ccagaagggc gggaaacagc tcgcggccga ggatgacggg gttaccgggg
     1261 ccgtcgctaa attcgggtcg gacggcgact tgctgctgcg gctcaaaggc ggatgctagc
     1321 tcgtcaagca gccagggcgg gacgagcggc tgatcaccga ggatgacggc agcagcgtcg
     1381 atgccggctg gaagagcggt caggccggcg cgcagcgatg acgcctgacc gctggcatag
     1441 tcggggttgt ggacaacgtc aaaaccgcgt ggatcgacgg cacggaggat gtcgtcggcg
     1501 tagccgccaa ggacgaggat tcgcggctcc aacgtcgtct ggcgcaccat gttgagcgtg
     1561 cactgcagga gcggtgtctg gccgaccggc agcagttgct tggggcggcc gaggcgggac
     1621 gagcttccgg cggcgaggac gatggcggcg acgcgggcag ccgtcacgag ccggtggtcg
     1681 ccttggccgg cgcgacggcg gcacgcgggt cacggcctcg gcgaactgcg acgatctcgg
     1741 ccatgatcga gagcgcgatc tcctccggag tcttgccgcc gatgttgagt ccgaccggac
     1801 cgtgaatccg atcaagctgc ttgtcggtga agcccgcccc cttgaggcgc tcgcggcgct
     1861 ccatgctcgt ggtgcgcgat ccaacggcgc cgatgtatcc ggcgtcggag cgtagcagca
     1921 cctccatggc cgggtcttcg aacttgggat cgtgggcgag gcagacagcg gcaacgccgg
     1981 ggtgcaggtt gagttgggcc agcgcctcgt ccggccaggc ctcgatcagc cggtcggcgt
     2041 gcgggaagcg ctcgtcggtc gcgaactggg cgcgggcgtc gatgacggtc gtccggtagc
     2101 cgagcgtctg gccaaaggtg acgagcggga tcgcgatgtg gacggcgccg acgatgacga
     2161 ggtgtggggg cggcaggtgc gcttccatga agacctgccg gccgtcgatt tcgtcgagcg
     2221 tcgtcccacc gtcgcgcagc atcgcgcgaa gcgttcgcga cgcctgctcg tcggcggccg
     2281 gcgaaccaag cccgccaagc acctgatccg ggtagacgat cagcaactcg ctgccggtat
     2341 ccgggccact gacgaccttg gcgt
//
LOCUS       DASUGJ010000388         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_614985, whole genome shotgun sequence.
ACCESSION   DASUGJ010000388 DASUGJ010000000
VERSION     DASUGJ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614985"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFV93_08530"
     CDS             complement(<1..904)
                     /locus_tag="VFV93_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX5165425.1"
                     /translation="MSQQATVEPPQVVATTPRRKKGTALTSTQTRTAWLLLLPTLLVV
                     AFIALYPLGETIYFSFTNKRLASVQPTEWVGLKNYRFLIEDTVFRDAIWTTVKFAIIT
                     LVAEFVLGLIIALVVNSNFKGRGFTRAAMLIPWAIPTVVSARMWAWMYNDIFGVFNDL
                     LHYRLHIIDRNIAWVAEDRYSLWSIAAVDIWKTTPFVALLLLAGLQVIPSDVYEAATV
                     DGANKFQQFWRITLPLLRPAILVTIIFRTLDALRVFDVFYVMFGARRSTQTMAVYAQN
                     NLVAFSDIGYGSAIAVGIFLIIGIF"
     gene            complement(1119..>2290)
                     /locus_tag="VFV93_08535"
     CDS             complement(1119..>2290)
                     /locus_tag="VFV93_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5165426.1"
                     /translation="SLTYSGDGVGIGNQLDKTLASKFTEATGIKIEVIPRPESSTETY
                     ATYQRTFEAKSGDIDVMMVDVIWPGAFAPHLLDLSDAFDGSMDQWYDSIVENNTIDGK
                     FVGAPWFGDFGILYYRTDLLEKYEYDGPPSTWDEFEEMANKIVEGEKASNPNFTGFVF
                     QGAAYEGLTCNALEWIASVGGGTIIEDGKVTVDNDDAKTMLDRVGGWVGTIAPTGVTG
                     YQEEDARNAFQGGNAAFMRNWPYAYSQGQEEGSAVKDKFDVGPLPVAEGQDPVGTLGG
                     WQLAVSAYSDAPEAAVEFVKYMVSPEVVKWRAVVATFVPLIIEVAEDPEVIANQPYLE
                     NLADVVRVTRPSQSTGENYNQVSTEFFQGVNEILTGGSADDIIPTVAQRIERLLG"
BASE COUNT          427 a          692 c          735 g          436 t
ORIGIN      
        1 cgaagatgcc gatgatcagg aagataccga cggcaatcgc gctgccgtag ccgatatcgg
       61 agaacgcgac caggttgttc tgggcgtaga cggccatcgt ctgcgttgaa cggcgcgcgc
      121 cgaacatgac gtagaacaca tcgaagacgc gcagtgcatc gagtgtccgg aagatgatcg
      181 tgacgaggat ggcgggccgc aagagcggca gcgtgatgcg ccagaactgc tggaacttgt
      241 tggcgccgtc gacagtggcc gcctcgtaga cgtcgctggg gatcacctgt aacccggcca
      301 gcaacagcag cgccacaaac ggcgtcgtct tccagatatc gacagcggcg atcgaccaga
      361 gggagtagcg atcctccgca acccaggcaa tgttgcgatc gatgatgtgc agcctgtagt
      421 gcaacaagtc gttgaagaca ccgaaaatat cgttgtacat ccaggcccac atccgggccg
      481 agacgacggt tgggatggcc cacggaatca gcatcgcggc gcgggtaaag ccacgtcctt
      541 tgaagttgga gttgaccacc agcgcgatga tgaggcccag gacgaattcg gcgacgagcg
      601 tgatgatggc gaacttgacg gtcgtccaga tcgcatcccg gaagacggtg tcctcgatca
      661 ggaagcggta gttcttcagt ccgacccatt cggtcggctg gacgctggcg agtcgcttgt
      721 tggtgaagct gaagtagatg gtctcgccga ggggatagag cgcgatgaag gcgacgacga
      781 gcagtgtcgg cagcaggagc agccaggccg tgcgagtctg cgttgacgtc agcgccgttc
      841 cttttttccg gcgcggtgtt gtcgcgacga cctgcggggg ttccaccgtc gcctgttggc
      901 tcatcgggcc ctcctcgcga tgggtagaca gcgacacggt gcgtccctgc ccatgacgaa
      961 acacgcgtgg aacgatcgga cgacgccgat atcaccctgc cgtccgtgcg ttgagttgcc
     1021 acatgaacgg cgagatggtg ccggcgcagt gctccccgac acggtaacgc ggatcgccga
     1081 atctgtgaag gcaggctcga cgatccgcgc tggtttgcct agccgagcag gcgctcgatg
     1141 cgctgcgcga cggttgggat gatgtcatcg gcgctgccgc cggtcaggat ctcgttgacg
     1201 ccctggaaga actcggtcga gacctggttg tagttctcac cggtcgactg cgacgggcgc
     1261 gtcacgcgga cgacgtcagc caggttctcc aggtacggct ggttcgcgat cacttccggg
     1321 tcttcagcga cctcaatgat gagcggcacg aacgtcgcca ccactgcccg ccacttgacg
     1381 acctcggggc tgaccatgta cttcacgaac tccacggctg cttccggcgc atcggaatag
     1441 gccgaaacgg ccagttgcca gccgccgagc gtaccgaccg ggtcctgccc ttcggcgacc
     1501 gggagcgggc cgacatcgaa cttgtccttg acggccgatc cctcttcctg gccctgcgag
     1561 taggcatatg gccagttgcg catgaatgcg gcattcccgc cctggaaggc gttgcgcgcg
     1621 tcctcttcct gatagccggt cacaccggtt ggggcaatcg tgccgaccca gccgccgacc
     1681 cggtcgagca tggtcttggc atcgtcgttg tcgacagtga ccttgccatc ctcgatgatc
     1741 gtgccgccgc ctaccgaggc gatccattcg agtgcgttgc aggtcaggcc ctcgtaggcc
     1801 gcgccttgga agacgaagcc ggtgaagttc ggattgctgg ccttctcgcc ctcaacgatc
     1861 ttgttcgcca tctcctcgaa ctcgtcccac gtcgacggcg ggccatcgta ttcgtacttt
     1921 tccagcaggt cggtccggta gtagaggatg ccgaagtcac cgaaccacgg tgcaccgacg
     1981 aacttgccgt cgatcgtgtt gttctcgacg atcgagtcgt accactggtc catcgagccg
     2041 tcgaaggcgt cgctcaggtc caacaggtgt ggagcgaacg cgcccggcca gatgacatcg
     2101 accatcatga cgtcgatatc gccggacttg gcctcgaagg tgcgctgata ggtcgcgtag
     2161 gtctcagtcg agctctccgg acgcgggatc acttcgatct tgatgccggt tgcctcggtg
     2221 aacttgctgg ccaatgtctt gtcgagctgg ttgccgatcc cgacgccgtc gccggagtac
     2281 gtgagcgacg
//
LOCUS       DASUGJ010000389         9497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1728086, whole genome shotgun sequence.
ACCESSION   DASUGJ010000389 DASUGJ010000000
VERSION     DASUGJ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728086"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VFV93_08540"
     CDS             <1..1139
                     /locus_tag="VFV93_08540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5165427.1"
                     /translation="DAGRGARDATIIVGLHAPPKPPLYAMRAMVYAARALRLDSFTVW
                     DHFQDLFPEALWGTDFTWLARDSDSPHEMFDYQTLLGYLAAHAGRLRLGVTVTEPVRR
                     HPVLLAQAMLTLAHVSKKAPILGIGSGEAENIVPYGLPFDQPVGRLEEALQILRLCFD
                     SHGPLDFQGTHFNLDHAFMDLPAPKRRKPAIWVAAHGPRMLRLAGQYGDGWYPTALAT
                     PADYAAGLEKVHAAAVNAGRDPARILPAFQAHVVVAPSMDEARRLFDSKPMRMIAILA
                     PAAIWRQFGQQHPFGDDFRGMTDFIPSRYSAQELNDALAAVPIEVAQEAMIWGTPERV
                     TERLRDFGAAGLRYVVIEVVSAAVSPKAAGYSLVALRKIAGALR"
     gene            complement(1164..2648)
                     /locus_tag="VFV93_08545"
     CDS             complement(1164..2648)
                     /locus_tag="VFV93_08545"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5165428.1"
                     /translation="MLANDQRSRDGHDPFASATRLAEALARREISSRELLDSYLGRVD
                     RHNPALNAVVTIDAERARAEADAADAAIARGEAIGPLHGLPVTIKDSLATAGLRTTSG
                     AGEFAEYVPDTDAVVVDRLRRAGAIVFGKTNLPTFAGDAQTYNDLFGTTNNPWDIERT
                     PGGSSGGSTAAIAAGLSALELGSDIGGSLRIPAHYCGVYTLKPTHGIVPERGHIPPLP
                     GSLSETDVMAIGPLARDARDLDLCLSVIAGPDVDRAVGWRLELPPPRHDALADYRIAV
                     WLDDPYCTIDGELAEVYATLVDELRRAGARISEVPPPVPLADAHDVAQRLIQGAMSRW
                     LPGDAFESLLARAAAAPAGDDTPPLRWARNITQRVRDHQLAAERRLQMKAAWAAFFRD
                     FDVFLCPVTPTAAIHHDQNPDVDARVIVVNGVPRPYGDQFAWLQSIGVVHLPAAVAPV
                     GLTSTGLPTGIQIVAPNLEDRTAIDIAQRIASVMGGFRAPPGFD"
     gene            complement(3102..4760)
                     /gene="hutU"
                     /locus_tag="VFV93_08550"
     CDS             complement(3102..4760)
                     /gene="hutU"
                     /locus_tag="VFV93_08550"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX5165429.1"
                     /translation="MTTGPRTIRAPRGTEISCKGWQQEAALRMLMNNLDPDVAERPED
                     LVVYGGTGRAARSWEAFDAIVASLRALENDETLLVQSGKPVGVLRTHADAPRVLIANS
                     LLVPKWATWEEFWRLEGLGLTMYGQMTAGSWIYIGTQGILQGTYETFGELARQHFGGT
                     LAGRVVLTAGLGGMGGAQPLSVTMNDGVCLVVEADPERIQRRLETRYVDEATESLEDA
                     IARVEEARSQNQPRSIALLGNAAEILPALVQMGFRPDVVTDQTSAHDALDGYIVPEMT
                     LEDARILRRHDSDEYLKRSLAAMGAHVEAMLAFQQAGSVVFDYGNNLRGQAYLAGVED
                     AFDYPGFVPAFIRPLFCEGQGPFRWVALSGDPADIVATDHALMELFPEKQHLHRWLTM
                     AEEQVAYQGLPSRICWLGYGERHLAGLRFNELVASGAVRAPIVIGRDHLDTGSVASPY
                     RETEGMLDGSDAIADWPILNALVNTASGATWVSVHHGGGVGIGLSIHAGQVIVADGTP
                     EAAARLERVLTNDPGTGVLRHVDAGYERAVMHARATGLRIPMAK"
     gene            complement(4955..5683)
                     /locus_tag="VFV93_08555"
     CDS             complement(4955..5683)
                     /locus_tag="VFV93_08555"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5165430.1"
                     /translation="MNTEDFQLPEARDAFLAASILLERIVPGCVVDDLDGLLHIHYGR
                     PKVGIEHEIVADDNDPVRTLAVAAAHPEATPAYLTTFAPLEHALTTAYEDAGLPRVVR
                     NTLMACRLPDVTPFADPSLRRLSDPDDLRSLALVRGDSVLDVEYFTDEIGCYALDIDG
                     ELVSSALLIPSAHGFGVIEHVHTLAAYRRRGYGRWLLRALHAEAARMGIHQVVLGSNV
                     AGRPLYTALGYVPLAHQDVYVVGG"
     gene            complement(5680..7212)
                     /gene="hutH"
                     /locus_tag="VFV93_08560"
     CDS             complement(5680..7212)
                     /gene="hutH"
                     /locus_tag="VFV93_08560"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX5165431.1"
                     /translation="MAVVVDGGHLTFEDVLAVARGGATIELGPNAWERMARSRAFVDA
                     AAADDRPVYGVTTGFGGLASMLIPADERVEMQHAILRSHAAGMGPPVEPEVARALVLL
                     RIATLAKGYSGIRPEVVERLVAILNAGIIPYIPSFGSLGASGDLAPLSHASLLLIGEG
                     QVLAEDGTPQPAGPVLAAAGIEPISLAAKEGLALINSTDGMLGMLVMACDDALRLFRT
                     ADIAAALSVEGLLGTDRPFAADLQALRPHPGQAASAANLRLLLAGSPLVAAHDDATHG
                     VQDAYSLRCHPQVIGAARDTLSFAVQTAERELASAIDNPVILDDGRVESAGNFHGEPL
                     AFACDFLTIAAAEVGAIAVARIDRLLDARRSRGLPPFLATNPGVNSGMMIAHYTAAAL
                     VAENRRLAHPASVDSMSTSANQEDHVSLGWAAARNLRQVLTNLARILAVEAVCAAQAI
                     DLRDPLQPAAATGAAIQTLRSHVPGPGPDRFLSPDLAAAEALVWSGELLSAVQKEAGG
                     LE"
     gene            7363..7923
                     /locus_tag="VFV93_08565"
     CDS             7363..7923
                     /locus_tag="VFV93_08565"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5165432.1"
                     /translation="MAVTLAQMTLEDFLSLPEAEPALEFEDGTVSQKVSPQGQHSRLQ
                     SEFMDHIRHQAESRKLAMAFPEIRTSYGERSYVPDIGVYRWDRIPRDATGRIVNDFRD
                     PPDIAVEIVSPEQRVTTLIRKCAWYIEHGVKIAMLIDPEDDSVMLFRPGRPLELLGGD
                     DKIALNDVLPDFDLTVRNLFESLYPD"
     gene            complement(7939..9078)
                     /gene="solA"
                     /locus_tag="VFV93_08570"
     CDS             complement(7939..9078)
                     /gene="solA"
                     /locus_tag="VFV93_08570"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEX5165433.1"
                     /translation="MNASYDTIVVGLGAMGSATVYQLAKRGQRVLGLEMFQPGHDQGS
                     SHGNHRMIRMSAVFADGYTPLAERALDLWHTLEEESGGTLLRLLGEIHLVDPASDPTY
                     AANAQEMRRRGLWEILSHEDLAERFPGVRLRDDMLATYEAKAGFLRSEAGILAHLAVA
                     ERHGATVVSGAEVTGWSVDGDGVRVTTGSETYTAERLIITTGPWAAELLADLNLPLRV
                     ERVVNGYFEPGRPDWWSAEQGAPDFLLTVPEGSFYGMPAVEGIGLKIGLSGGFGLGVT
                     TARTIDRHVDESEIDFLRTVLDTYMPGASGQALRHVTCMCTYTPDKGFVVDRHPAHEQ
                     VLIGCGFSGRGYKFAPTVGEILADLATAGATRHDINFMSATRFGR"
BASE COUNT         1605 a         3079 c         3284 g         1529 t
ORIGIN      
        1 aggacgcggg acgcggggcg cgggacgcga cgatcatcgt tgggctgcat gcgccgccga
       61 agccgccgct gtatgcgatg cgggcgatgg tgtatgccgc gcgggcgctg cggctggatt
      121 cgtttacggt ctgggaccac tttcaggacc tgttccccga agcgctctgg gggactgact
      181 tcacgtggct ggcgcgtgac agcgactcgc cgcacgagat gttcgactac cagacgttgc
      241 tgggctatct ggcagcccac gccgggcggc tgcggctggg cgtgacggtg acggagccag
      301 tccgccggca tccggtcctg ctggcgcagg cgatgctgac gctggcgcat gtctcgaaga
      361 aggcgccgat tctggggatc ggcagcggcg aggcggagaa catcgtcccc tacgggctac
      421 cgttcgacca gccagtcggg cggctggagg aggcgctcca gatcctgcgg ctctgcttcg
      481 acagccacgg gccgctcgac tttcagggca cgcacttcaa tcttgaccac gccttcatgg
      541 acctgccggc gccgaaacgc cgcaagccgg ccatctgggt cgcggcgcac ggcccacgga
      601 tgctgcggct ggcggggcag tatggcgacg gctggtaccc gaccgcgctc gcgaccccgg
      661 ctgactacgc ggctgggctt gagaaggtcc acgccgccgc cgtcaacgcc ggacgtgatc
      721 cggcccggat tctgccggcc ttccaggcgc atgtcgttgt cgcgccaagt atggacgagg
      781 cacggcgact gttcgacagc aagccgatgc ggatgatcgc gatcctcgcg ccggccgcga
      841 tctggcgaca gttcggccag cagcacccgt tcggcgatga ctttcgcggc atgaccgact
      901 tcatcccatc gcgctacagc gctcaggagc tcaacgacgc gctggcggcc gtcccgatcg
      961 aagtcgccca ggaggccatg atctggggca cgccggagcg ggtgaccgag cgcctgcgcg
     1021 acttcggcgc ggccggcctg cgctacgtcg tgatcgaggt cgtctcggcc gccgtctccc
     1081 cgaaggcggc cgggtattcg ctggtggcct tgcggaagat tgcgggggcg ttgcggtagt
     1141 gagaaacccc aacccaacct gactcaatcg aacccgggcg gcgcccgaaa tcctcccatg
     1201 acgctggcga tgcgctgggc tatgtcgatc gccgtccgat cttcgaggtt gggcgccacg
     1261 atctggatgc cggtgggtaa gcctgtcgag gtcaggccga cgggggcgac ggcggccggc
     1321 agatggacga cgccaatcga ctggagccac gcgaactggt cgccgtatgg acgggggacg
     1381 ccgttcacaa cgatgacgcg ggcgtcaacg tcgggattct ggtcgtggtg aatggcggcg
     1441 gtcggcgtca cggggcagag gaacacgtcg aagtcacgga agaacgcagc ccaggcggct
     1501 ttcatctgca accgtcgttc cgccgcaagc tgatggtcgc gcacccgttg ggtgatgttg
     1561 cgcgcccagc gcagcggtgg ggtgtcgtcg ccggccggcg ctgcagcggc acgggccaaa
     1621 agcgactcga acgcatcccc cggtagccag cgtgacatgg cgccctggat gaggcgttgg
     1681 gccacatcgt gggcatcagc cagcggaacc ggagggggaa cctcagaaat ccgcgcgccg
     1741 gcgcgtcgaa gctcatcgac gagcgtggcg tagacctcgg caagctcacc atcgatcgtg
     1801 cagtacgggt catccagcca gacggcgatg cgatagtccg cgagcgcgtc gtggcgtggt
     1861 ggaggaagct cgagccgcca gccgacggct cgatcgacgt ccgggccggc gatcaccgac
     1921 aggcagaggt cgaggtcacg ggcgtcccgc gccagcggcc cgatcgccat cacgtcggtt
     1981 tccgaaagag agccaggcag cggcgggatg tggccccgtt caggaacgat gccgtgggtc
     2041 ggcttgagtg tgtagacgcc gcagtagtgg gccggtatgc gcaacgaccc gccgatgtcg
     2101 ctgccgagct ccagtgcgct gagtccggct gcaatggccg ctgtcgaacc gcctgacgag
     2161 ccgcccggcg tccgttcgat gtcccacgga ttgttggtcg tgccgaagag gtcgttgtag
     2221 gtctgtgcgt cgccggcgaa ggttggcagg ttcgtcttcc cgaagacgat cgcaccggca
     2281 cggcgcaggc gatcgaccac caccgcgtcg gtgtcaggaa cgtactcggc aaactcgccg
     2341 gctccggagg tcgtgcgcag ccccgccgtc gcgagcgagt ccttgatcgt gactggcagc
     2401 ccgtggagcg ggccgattgc ctcgccgcgc gcaatggcag cgtcggccgc gtccgcctcg
     2461 gcacgtgcgc gctcggcgtc gatggtcacg acggcgttga gtgccgggtt gtgacgatcg
     2521 acccggccga gatagctgtc gagcagttcc cggctggaga tctcccgacg agcaagtgcc
     2581 tcggcgagtc gggtggctga ggcgaagggg tcgtgtccgt cgcgtgagcg ttgatcgttg
     2641 gcaagcatgc tgtcctcctg cacgttcgac gttcgaccgg gaatgcgatc actcccatcg
     2701 tgacaccacg gtaagcgcaa actcttacgt ctcgattgcg accggccacg cggatatctt
     2761 cacgcctgtc gccagaacgc gcaagaaggc ggtgccgtcg ttggccgatg cgagacggca
     2821 ggttcgggaa tcgggaatcg ggattcgggg gcggatgacg gcgctgcctt cccagacggc
     2881 cggcgcttct gaggatcggc gcgcctcacc cctgtcatcc tgagccgcgg cgaaggatcg
     2941 cccagcgttt gggttacggt gggccaccga ggatgcacac gttcgcctgc cgaattagcc
     3001 caatacgtca ctcacgccag gcgatccttc gctgcggctc aggatgacag gggtcgcgtg
     3061 tgcggtcctc atgaacactg tcccgcgtcc cgcgtcccga actacttcgc catcggaatc
     3121 cgcaaacccg tcgccctcgc gtgcatgacg gcccgttcgt agccagcgtc gacgtggcgc
     3181 aagacgccgg tgccaggatc gttcgtcaac acccgttcga ggcgggcggc agcttccggg
     3241 gtgccgtcgg cgacgatgac ctgtccggcg tggatcgaga ggccgatgcc gacgccgccg
     3301 ccgtggtgga ccgagaccca ggttgcgccg gaggcggtgt tgaccagcgc gttgaggatc
     3361 ggccagtcgg cgatcgcgtc ggagccgtcg agcatgccct ccgtctcgcg gtagggcgag
     3421 gcgaccgagc cggtgtcgag atggtcgcgg ccaatcacga tcggggccct gacggcaccg
     3481 ctggcgacca gttcgttgaa ccggagtccc gccaggtggc gctcgccgta gccgagccag
     3541 cagatgcgcg acggcaaccc ctgataggcg acctgctcct cggccattgt cagccagcgg
     3601 tggagatgct gcttctccgg gaacagctcc atcagggcgt ggtcagtcgc gacgatgtcg
     3661 gccgggtcgc cggagagtgc cacccagcgg aacggcccct gtccctcgca gaagagcggc
     3721 cggatgaagg ccgggacgaa gccggggtag tcgaaggcgt cctcgacgcc ggccagatac
     3781 gcctgaccgc gcaggttgtt gccgtagtcg aagacgaccg agccggcctg ctggaaggcg
     3841 agcatcgcct cgacatgcgc gcccattgcg gccagcgagc gcttcaggta ctcgtcgctg
     3901 tcgtggcgac gcaggatacg ggcatcctcc agcgtcatct ccgggacgat gtaaccatcc
     3961 agcgcgtcgt gggcgctggt ctggtcggtg acgacatccg ggcgaaagcc catctggacg
     4021 agggccggca ggatctcggc cgcatttccg agcagcgcga tcgagcgcgg ctggttctgc
     4081 gagcgcgctt cctcgacacg ggcgatggcg tcttccagcg attcggtcgc ctcgtcgacg
     4141 tagcgggttt cgagccggcg ctggatgcgc tcgggatcgg cctcgacgac caggcagacg
     4201 ccgtcgttca tcgtgaccga gagcggctgg gcgccaccca tcccgccgag accggccgtc
     4261 aggacgaccc ggccggcgag cgtgccgccg aagtgctgcc gcgcaagctc gccgaacgtc
     4321 tcgtaggtgc cctgaaggat gccctgggtg ccgatgtaga tccacgagcc ggccgtcatc
     4381 tgaccgtaca tcgtcaggcc cagcccctcc agccgccaga actcctccca ggtggcccac
     4441 ttcggcacca gcagcgagtt ggcgatcaga acacgggggg cgtcggcgtg cgtccgcagg
     4501 actccgaccg gcttgcctga ctggaccagc aacgtctcgt cattctccag cgcgcgcagg
     4561 ctggcgacga tcgcatcgaa cgcctcccag gagcgggccg cccggccggt cccgccgtag
     4621 acgaccagat cctccggccg ctcggccaca tcggggtcga ggttgttcat caacatccgc
     4681 agggcggctt cctgctgcca gcccttgcag gagatctcag taccgcgcgg ggcgcggatt
     4741 gttcgcggtc cggttgtcat gggtgactcc tttcgtgtcc tgacggcctg accgggcatg
     4801 aacggcgagg cagcaccatc gccagacgcg gtcagccagc cgcccgcatc atcgcatgca
     4861 acgtgggaag cgtgtcagct ggcgtgcgtg cgatgcagga tcacgccctc cgtaggggcc
     4921 gcggccccta cggagggcgt gtcatctccg gcgttcagcc gccaacgacg tagacatctt
     4981 gatgggccag cggaacgtat ccgagtgcgg tgtagagtgg gcggccggcg acgttcgagc
     5041 cgagcacaac ctgatggatg cccattcggg ccgcttcggc gtgcagcgcg cgcagcagcc
     5101 agcgaccgta gccgcgacgg cggtaggcgg ccagcgtgtg aacatgctcg atgacgccga
     5161 agccgtgggc agatggaatc agcagcgccg aggagacgag ttcgccgtcg atgtcgagcg
     5221 cgtagcagcc gatctcgtcg gtgaagtact cgacatccag aacgctgtcg ccacggacca
     5281 gcgcgagcga gcgcaggtcg tcggggtcgg acaaccgcct gagcgacgga tcggcgaacg
     5341 gcgtcacatc cggcaaccgg caggccatca gtgtgttgcg aacgacccgt ggcaggccgg
     5401 cgtcctcgta ggcggttgtc agcgcgtgct ccaacggcgc gaaggtcgtg aggtaggccg
     5461 gtgtcgcttc cggatgggcg gccgcgaccg ccagcgttcg gaccgggtcg ttgtcatcgg
     5521 cgacgatctc gtgctcgatg ccgactttcg ggcggccgta gtggatgtgg agcaggccgt
     5581 cgaggtcgtc gacgacgcag ccgggcacga tccgttcgag caatatcgac gccgccagga
     5641 aagcatccct ggcctcgggc aactggaaat cttccgtgtt cattccaacc cgccggcctc
     5701 tttctggacg gcggacagca gctcaccgct ccagaccagc gcttcggccg ccgccagatc
     5761 cggcgacagg aagcggtccg ggccgggacc gggaacgtgc gagcgcagcg tctggatggc
     5821 ggcgccggtc gcggcggccg gctggagggg gtcgcgcagg tcgatcgcct gggcagcgca
     5881 gacggcctca accgccagaa tccgggcgag gttggtgagc acctggcgca ggttgcgggc
     5941 ggctgcccat ccgagcgaga cgtgatcctc ctgattcgcg gaggtcgaca tcgaatcgac
     6001 gctggccggg tgagccaggc ggcggttctc ggcgaccaga gcggcggcgg tgtagtgggc
     6061 gatcatcatg cctgagttga ccccggggtt ggtcgccagg aacggcggca ggccacgcga
     6121 acgcctggcg tccagcagcc ggtcgatccg ggcgacggcg atcgcgccaa cttcggcggc
     6181 ggcgatcgtc aggaagtcgc aggcgaaggc cagcggttcg ccgtggaagt tgccggccga
     6241 ttcgacccga ccgtcatcca gaatcaccgg gttatcgatc gcggatgcca gctcccgctc
     6301 ggcggtctgg acagcaaagc tgagcgtgtc gcgggcggcg ccgatcacct gcgggtgaca
     6361 gcgcagcgag taggcatcct ggacgccgtg agtagcgtcg tcgtgggccg ccaccagcgg
     6421 tgagccggcc agcagcaggc gcaggttggc ggcgctggct gcctggcccg gatgggggcg
     6481 cagcgcctgc agatcggcgg caaacggtcg atccgtgccg agcagccctt cgacgctcag
     6541 ggcggcggca atgtcggccg tccggaacag ccgcagggcg tcgtcgcagg ccatcaccag
     6601 catcccgagc atgccgtcag tgctgttgat gagggccagc ccctccttgg cggccagcga
     6661 aatcggctcg atgccggccg ctgcgaggac cgggccggcc ggctggggcg tgccatcctc
     6721 tgccagcacc tgcccctcgc cgatcagcag cagtgaggcg tgcgagagcg gcgcgaggtc
     6781 accgctggcg ccgagcgagc cgaatgaggg gatgtagggg atgatgccgg cgttgaggat
     6841 cgcgaccagc cgctcgacga cctcgggccg gatgccggaa tagcctttgg ccagcgtcgc
     6901 gatccgcagc agcacgagcg cccgcgccac ttccggctca accggcggtc ccattccggc
     6961 tgcatgcgaa cgcaggatcg cgtgctgcat ctcaacccgc tcgtcggccg gaatcagcat
     7021 gctggccaac ccgccgaagc cggtcgtcac accatagacc ggccgatcat cggccgcagc
     7081 ggcatcgacg aacgcccgcg aacgcgccat ccgctcccag gcgttcggcc cgagttcgat
     7141 cgtcgcgcca cctcgcgcaa ccgccaggac atcctcaaac gtcagatgcc caccatcaac
     7201 gacaactgcc acgctccacc ccctcaccag ttaccgctag tataggacgg tcatgcagaa
     7261 gacgaaggcg gctgcgcgct gcgtgctaca ctccaaaagc cggtcgcagt cctgcttcgt
     7321 agacacaact gacgccctgc accgccggat cggagcccga caatggcagt caccctagct
     7381 cagatgacac ttgaagactt cctgtcgctt ccagaagcgg agcctgcact cgagttcgaa
     7441 gacgggacgg tgtcgcagaa ggtgtcacca caaggacagc acagcagatt gcagtccgag
     7501 ttcatggatc atattcgtca tcaggcggag tcacggaagc tggcgatggc atttccggag
     7561 attcggacga gctacggcga acggtcctat gtgccagata ttggtgtgta tcgctgggat
     7621 cgcattcctc gcgatgcaac cggtcgtatt gtgaacgact ttcgagatcc gccggatatc
     7681 gctgtagaaa tcgtctctcc agagcaacgc gttaccacac tcattcgtaa gtgcgcatgg
     7741 tacatcgagc atggcgtgaa aatcgcaatg ctgatcgatc ctgaagacga ttcagtcatg
     7801 ctgttccgcc caggacgccc gcttgagttg cttggcggcg atgacaagat cgcgttgaat
     7861 gatgtgcttc ccgatttcga tctgacggtt cgaaacctgt ttgaatctct gtatccagac
     7921 tgatgcacgg aagccgcttc atctaccgaa ccgtgtagcc gacatgaagt tgatgtcgtg
     7981 gcgggtggcg ccagcggtgg cgaggtcggc caggatctcg ccgacggtcg gcgcgaactt
     8041 gtagccccgg ccggagaagc cgcagccgat cagaacctgc tcgtgggctg gatggcgatc
     8101 gacgacgaac cccttgtcgg gcgtatacgt gcacatgcag gtgacgtggc gcaacgcctg
     8161 accgctcgcg ccgggcatgt aggtgtcgag gacggtgcgg aggaagtcga tctcggattc
     8221 gtcgacgtgg cgatcaatcg tgcgtgcggt cgtgacgccc agtccgaagc cgccgctgag
     8281 gccgatcttg aggccgatcc cctcgacggc cggcatcccg tagaagctcc cctccgggac
     8341 ggtcagcagg aaatccggtg cgccctgctc agctgaccac cagtcggggc ggccaggctc
     8401 gaagtagccg ttgacgaccc gctcgacccg cagcggcagg ttcagatcgg ccagcagctc
     8461 agccgcccag ggaccggtcg tgatgatgag acgctcggcc gtgtacgtct cgctgccggt
     8521 tgtcacgcga acgccatcgc catcgacgga ccagccggtc acttcggcgc ccgagacgac
     8581 ggtcgcgccg tgccgttcgg cgacggccag atgggcgaga ataccggctt ccgagcggag
     8641 gaagcccgcc ttcgcctcgt aggtggcgag catgtcgtcg cgaaggcgta cgccaggaaa
     8701 gcgctcggcc aggtcttcgt ggctaaggat ctcccagagg ccgcgccggc gcatctcctg
     8761 ggcgttcgca gcataggtag ggtcgctggc cgggtcaacg agatggatct cgcccagcag
     8821 ccgtagcagc gtcccgcccg actcctcttc gagggtgtgc cagaggtcga gggcgcgttc
     8881 tgcgagcggc gtatagccat cggcgaagac ggccgacatc cggatcatgc ggtggttgcc
     8941 gtgggatgag ccctgatcgt gacctggctg gaacatctcc aaaccaagca cgcgctggcc
     9001 acgtttggca agctggtaga ccgtcgcgct ccccatcgcg cccaggccaa ccacgatcgt
     9061 gtcgtacgaa gcattcatgg tcgtcattct ttcgactacg cgagcgttcg gcggtgaggg
     9121 cgatggtacc cggatttggt ggcgaaaacc cctccccaac cgccacccag agggtacccg
     9181 gcctgcgagg agaggggctt ttggccgcac gaaatctaag cccctctcct cgcaggggag
     9241 gggttttcac cacccagcga ctggacgacg gacccgtcct ctgtcacagt tgcagccggc
     9301 cgccaagccg ccatcgctca gagtgaaacg agggaacaat gagccagtcc gtgacgacca
     9361 cgaaccatcc ggtgcgcttc ggcctgcgct ccagccagaa tcaccgcagt tggtcgcaga
     9421 tccgcgaggc gttcgtggcc gccgatgagt gggggctgga atcggcctgg gtgttcgacc
     9481 acatgatccc gctctcc
//
LOCUS       DASUGJ010000390         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2005793, whole genome shotgun sequence.
ACCESSION   DASUGJ010000390 DASUGJ010000000
VERSION     DASUGJ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005793"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFV93_08575"
     CDS             <1..534
                     /locus_tag="VFV93_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5165434.1"
                     /translation="ADEVADDAVDDSTELEAVAAEDDDAAETWALVNSGELPPAFDRE
                     QVRDLLIDAAHRYGWDPYLIMAQAWQESYWSQNELSWTGAVGVMQVMPDTSAEMESWY
                     FGYQLDTWYSAYDNIEMGVAYLTVLYNETGSVENALASYYQGWGSLQRDGWFPDTEEY
                     VQRIFRFREMFAAGETP"
     gene            complement(614..1372)
                     /locus_tag="VFV93_08580"
     CDS             complement(614..1372)
                     /locus_tag="VFV93_08580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5165435.1"
                     /translation="MCHPDTSMTGATQPARPSSEESFTIEVAGASMPVFQASPARPSR
                     GNVIVIHDIHGANAFYHDLARRLASEGYTAYLPDLFVREGPLPEPTREYASARGQKLS
                     YPTTIDDLKTLFGVVAQRSPGKVATVGFCMGGTLVMLLASREPRMAAGVIYYGFPANA
                     NISEKRPWQPLDEAATVETPLLGFWGDQDKGVGMDNVAAYTAGLDAAGKPHDFTIYPG
                     LPHGFLTFDSASPNYAGSQDSWQKALAYFGDRLG"
     gene            1445..1951
                     /locus_tag="VFV93_08585"
     CDS             1445..1951
                     /locus_tag="VFV93_08585"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5165436.1"
                     /translation="MTASAMIRTASTGDIPAIRRVAQVTWRATYADQIAAEDIEQFLA
                     SAYSLPSLTTRLARFGDGFIVAALDRKVIGYAMAGLNRDGEPELYAIYVLPEHHGVGA
                     GHLLWNAAKDVLVRQGYARMCCWVMSGNVGARRFYERQGAIMTEEREFPVGATMVREA
                     RYCAAIRD"
     gene            1977..>2526
                     /locus_tag="VFV93_08590"
     CDS             1977..>2526
                     /locus_tag="VFV93_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165437.1"
                     /translation="MPITSRQLRDEDDYQALRAMLQRLHTAGGQPDYCTIGDLDWWRY
                     THEDPEAIRQARLWLDDRGMVVGFAWPSGDHLDHVVDPDQRDLEAEMLTWAETQIRAS
                     GQHTSMTAFANDGDTGRQAQLQALGFERADEAFRYWQRTLDHLPDTQLPGDYVIRNLR
                     GEVDVAARVAAHRDAFAPSKMTE"
BASE COUNT          437 a          780 c          859 g          450 t
ORIGIN      
        1 gccgatgaag tggctgatga cgccgtcgac gattcgaccg agcttgaagc agtcgctgcg
       61 gaagatgacg atgcggcaga gacgtgggca ctggtgaact cgggtgagtt gccgccggcc
      121 ttcgatcgcg agcaggttcg cgatctactg attgacgccg ctcaccgcta cggttgggac
      181 ccatacctga tcatggctca ggcctggcag gagagctact ggtcgcagaa tgagctgtca
      241 tggacgggcg ccgtcggagt catgcaggtc atgccggaca cgtccgccga gatggagtcg
      301 tggtacttcg gctaccaact cgacacctgg tactcggcct acgacaacat cgagatgggc
      361 gtcgcctatc tgacggtcct ctacaacgag accggcagcg tcgagaacgc ccttgccagc
      421 tactaccagg gctggggcag cctccagcgc gatggctggt tcccggacac cgaagagtac
      481 gtccagcgca tcttccgctt ccgcgagatg ttcgcggcgg gcgagacgcc gtaactgagg
      541 gtgtcgcaac ccatacagaa gccggagccg gtccaggacc gactccggct ttcgcatgtg
      601 acgtgatccg ccgctacccc agccggtcgc cgaagtacgc cagtgccttc tgccagctat
      661 cctggctgcc ggcgtagttg gggctggcgc tgtcgaaggt caggaatccg tgcggcaggc
      721 cggggtagat cgtgaagtcg tgtggcttgc cggccgcgtc cagcccggcg gtgtaggcgg
      781 cgacgttgtc catcccaacg cccttgtcct gatcgcccca gaagccgagc agcggagtct
      841 caactgtcgc ggcctcgtcg agcggttgcc agggacgctt ctccgagatg ttcgcgttgg
      901 ccgggaagcc gtagtagatc acgccggcgg ccattcgcgg ctcacgcgat gccagcagca
      961 tgacgagcgt gccgcccatg cagaagccga ccgtggcgac cttgccgggc gagcgctgcg
     1021 cgacgacgcc gaacagtgtc ttgagatcgt cgatcgtggt tggatacgag agcttctgcc
     1081 cgcgtgccga tgcgtactcg cgggttggtt ccggcagcgg tccttcgcgg acgaagaggt
     1141 cgggcaggta ggcggtgtag ccctcgctcg ccagccggcg ggccaggtcg tgatagaagg
     1201 cgttcgcgcc gtggatgtcg tggatgacga tcacgttccc ccggctcggc ctggctgggg
     1261 acgcctggaa gacaggcatc gaggcgccgg cgacctcgat cgtgaagctt tcctcgctcg
     1321 agggcctggc cggctgcgtt gcgccggtca tacttgtgtc tgggtggcac acgatcgcga
     1381 ctcccttcgt cattgactgg tgtgtcgccc ggcacaatag cacagtccgg gaaggctcat
     1441 gccgatgact gcttccgcaa tgattcggac ggcgagcacc ggtgacattc ccgcgatccg
     1501 gcgcgtcgcg caggtgacgt ggcgagcgac ctacgctgac cagatcgccg ccgaggacat
     1561 cgagcagttc ctcgcctccg cctattcgct gcccagcctg accaccaggc tcgcgcggtt
     1621 cggcgacggg ttcatcgtcg ccgccttgga cagaaaggtg atcggctatg cgatggcagg
     1681 gttgaaccgg gacggcgagc cggaactgta tgcgatctac gtcctgccag agcatcatgg
     1741 agttggcgcc ggacacctgc tctggaacgc tgccaaggac gtgctggtac gtcagggtta
     1801 tgcgcggatg tgctgctggg taatgtcggg taatgtcggt gcgcgccgct tctacgagcg
     1861 ccagggcgcg atcatgacgg aagagcggga gttcccggtt ggcgcgacga tggtccgtga
     1921 ggcacgctac tgcgccgcga tcagggattg aagcaggaac gacacaggag gacattgtgc
     1981 cgatcacgtc tcggcagctt cgtgacgagg atgactacca ggcgcttcgc gcgatgctgc
     2041 aacgcctcca cacggccggc gggcagccag actactgcac gattggcgac ctcgactggt
     2101 ggcgttacac gcacgaagac ccggaggcga tccggcaggc gcggctctgg ctcgacgatc
     2161 gcgggatggt cgtcgggttc gcctggccga gcggcgacca cctcgaccac gtcgtcgacc
     2221 ccgatcagcg cgatcttgaa gcggagatgc tcacctgggc cgaaacgcag atccgcgcct
     2281 caggtcagca cacctcgatg acggcgtttg ccaacgacgg cgacaccggg cgacaggcac
     2341 agcttcaggc gttgggtttc gagcgggccg atgaagcgtt ccgctactgg cagcggacgc
     2401 tcgatcatct acccgacact cagctgcctg gagattatgt cattcgcaac ctgcgtggcg
     2461 aggtcgatgt cgccgcgcgc gttgccgccc atcgcgatgc gtttgctcca tcgaagatga
     2521 ccgagg
//
LOCUS       DASUGJ010000391         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_59711, whole genome shotgun sequence.
ACCESSION   DASUGJ010000391 DASUGJ010000000
VERSION     DASUGJ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59711"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..690)
                     /gene="greA"
                     /locus_tag="VFV93_08595"
     CDS             complement(220..690)
                     /gene="greA"
                     /locus_tag="VFV93_08595"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5165438.1"
                     /translation="MPDRSVPLTRDGLKKLEDELDYLKSVRRHEVAQRIHMAKEVGHT
                     QNDAEYDDAKNEQAFVEGRINQLETIVKNHVLIDEDQAHHSRSVRLGCTVVVESASGR
                     REYTIVGSTEADPTSGRISNESPVGKALIGHRVGDKVQVLTPKGPASFTVSEIK"
     gene            1007..2152
                     /locus_tag="VFV93_08600"
     CDS             1007..2152
                     /locus_tag="VFV93_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5165439.1"
                     /translation="MEDRVSPWRGVMGILAIAVAVAAISGAFAGASVALLVSDNESDA
                     KPDAAAAEPGPSTLLKVTEESALTETFSKVSPGVVTLIVQAQHLDSNGRVVRETNLGS
                     GVIVDERGYLVTNEHVVRDATQISVIMNDGSERPGVLVGDDAPFTDVAVVRIQPSGIT
                     VVPVGDSNSVQPGQGVLAIGSVAFNQQMYDQFRNNVTRGVVSGVHRRWPKDDTVMEDL
                     IQTDAAINHGNSGGALVTLDGQLIGIMTTVVRGTQSGQQVQGVGFAISSRSFKPLIDQ
                     IIANGKTDRPYVGIVHQTISADQAQRARLPVTNGAAAVVDVIDNSPASRAGVQKGDII
                     TKLADYEINDDQPYLYALVKMKPNTTVPLTVLRGGQEMQLSLDILNR"
     gene            2279..>2729
                     /locus_tag="VFV93_08605"
     CDS             2279..>2729
                     /locus_tag="VFV93_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165440.1"
                     /translation="MNRPPPRRPGTSQRRPAAGGGPLRDPRLLAIGGGGLLLIVLLAG
                     LFLGPCSPLNPSDGGDETLFNCPTIKDPPAPPDGLEIASDFKNFSDDDCNRMIPEGVA
                     KISMPVEQGRAGRGLLFYTYQNDSWQRLATAELTDDGKYAEIVVDQVP"
BASE COUNT          527 a          834 c          869 g          499 t
ORIGIN      
        1 agggtcccgc cagtttcacc gtgtccgaga tcaagtaagg aacccctccc ccgacccctc
       61 cccccgctga cgcgcggaga ggggagcctc gtctgggcgt ggtgcaaacg gcgaaccatc
      121 ccaccgccag accgttgcga agacggcgcc ggcacccacc gtcagaccgt actcccctct
      181 ccgcgcgtca gcggggggag gggccggggg aggggttcct tacttgatct cggacacggt
      241 gaaactggcg ggacccttcg gcgtcaacac ctgcaccttg tcgccgacgc gatggccgat
      301 caatgccttg ccgaccggcg attcgttgga gatacggccg ctggttggat cggcctctgt
      361 gctgccaacg atggtatatt cccgacggcc gctcgcggac tcgacgacga ccgtgcaacc
      421 aagccgcacg gaccgagagt gatgcgcctg atcctcgtca atgaggacgt gattcttgac
      481 aatcgtttca agctggttga ttcgaccctc gacaaaggcc tgttcgttct tcgcgtcatc
      541 atactctgcg tcgttttgcg tgtggccgac ttccttcgcc atatgaatgc gctgggcaac
      601 ctcgtgccgg cgcaccgact tgaggtagtc gagctcatcc tccagctttt tcaatccgtc
      661 tcgtgtcagt ggtacggagc ggtcaggcat cacacgactc ccaaaacgac gagaaaaaag
      721 aagctgaggg cacacgtgcc cgcagctact cgcttcatgg agtatagaga tggtccaaga
      781 ggcctgtcaa agacgtacca attcccctag tcgcggcgaa tcccgtcgaa tcggatcaac
      841 gaggcagttc gcgcggtgcg ggttacttat gccgcgctcc gtggatctag gcactgggaa
      901 gccatgcggt gcagtccact gggacatgaa gtgggcgtca cgtccttgtt acaatactcc
      961 tgccggccgc aatcgccccg gatgtggtgc ggaggggaga ggatcgatgg aggatcgggt
     1021 gtcgccctgg cgcggggtga tgggaatcct ggcgatcgcg gtggcagtgg ccgcgatcag
     1081 tggcgcgttc gccggcgcga gcgtggcgct tctggtatcg gataatgagt cggatgcgaa
     1141 acctgatgcg gcggcggcgg aaccggggcc atcgaccctg ctgaaagtga cagaggagtc
     1201 ggcgctcacc gagacgttca gtaaggtgtc gcccggcgtt gtcacgctga tcgtgcaggc
     1261 gcaacacctc gacagcaacg gtcgcgtcgt tcgggaaacc aacctcggat cgggcgtgat
     1321 cgtcgacgag cgcggttatc tcgtgacgaa cgagcacgtc gttcgtgacg ccacgcagat
     1381 ttccgtgatc atgaatgacg ggagcgagcg accgggcgtg ctcgtcggag acgacgcgcc
     1441 ctttaccgac gtcgccgttg tgcgtatcca gccgagcggc atcactgtgg taccggtcgg
     1501 agactcgaac tcggtacagc ccggtcaagg tgtgctggcg atcggcagcg tcgccttcaa
     1561 tcagcagatg tacgaccagt ttcgcaataa cgtcacccgc ggcgtcgtta gcggtgtcca
     1621 tcgccgctgg ccgaaggatg acacggtgat ggaggatctg atccagaccg atgcggcgat
     1681 caaccacggt aacagcggcg gcgcgctcgt gacgctcgat ggacagctga tcggcatcat
     1741 gacgaccgtc gtccggggga cgcaatccgg ccagcaggtg cagggcgtcg ggtttgcgat
     1801 ctccagccgc tcgttcaaac cactgatcga tcagatcatc gcgaacggca agaccgaccg
     1861 accgtacgtc ggcatcgtac accagacgat cagcgccgat caggcgcagc gggcacggct
     1921 acccgtgaca aatggggcgg cggcggtcgt cgacgtgatc gacaatagcc ctgcctcccg
     1981 tgccggcgtc cagaagggcg acatcattac gaaactggcc gactatgaga tcaacgacga
     2041 tcagccatac ctctatgcgc tggtcaagat gaagccaaac acgacggtac cgctgacggt
     2101 cctccgtggt gggcaggaga tgcaactgag ccttgacatc cttaatcgtt gaacgtgaga
     2161 acagccggtc gatgttgggc gttcccatga atttccttga ccgtccacga cgccattcgt
     2221 acgctgccgt tggtacacct gccgcgtgct gttccggctg ggggagagat cagattcaat
     2281 gaaccgaccg cccccgcgcc gtccggggac aagccagcga cgaccggccg ccggcggtgg
     2341 tccgctccgc gacccgcgtt tgctcgcgat cggaggcggc ggactgctcc tgatcgtcct
     2401 gctggcaggc ctctttctcg ggccgtgttc gcccctgaac ccgtctgatg gcggcgacga
     2461 gaccctgttc aattgtccaa cgatcaagga cccgccggcc ccaccggacg gacttgagat
     2521 cgccagcgat ttcaagaatt tcagcgacga cgactgcaac cggatgatcc cggagggtgt
     2581 cgccaaaatc tcgatgccgg tggagcaggg gcgggccgga cggggtctgt tgttctatac
     2641 gtatcagaat gactcctggc aacgactcgc cacggcggag ctcaccgacg acggcaagta
     2701 cgccgaaatc gtggtcgatc aggtgccgg
//
LOCUS       DASUGJ010000392         4147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1448295, whole genome shotgun sequence.
ACCESSION   DASUGJ010000392 DASUGJ010000000
VERSION     DASUGJ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448295"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..806)
                     /locus_tag="VFV93_08610"
     CDS             complement(237..806)
                     /locus_tag="VFV93_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5165441.1"
                     /translation="MASYWEGSRVRLRAIEPDDWQIHHAWDQDSTSQRLLDRVHLPRS
                     REGTRRWAEQKAASEPEGDNVHLEVETLEGVHVGSVAAHDCDRRTGIFELGIAIRPEH
                     QRRGYAREAIALLLRYYFHELRYQKVTAFIVDINQPSLRLFERLGFQQEGRLRRLVYI
                     SGRHYDYIAYGMTIEEFDASGLIAPFSPE"
     gene            complement(832..1668)
                     /locus_tag="VFV93_08615"
     CDS             complement(832..1668)
                     /locus_tag="VFV93_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5165442.1"
                     /translation="MATQATTLNRAPTREYSPVGFGATLASEWTKMFSTRSTYVMLGL
                     ALVLSFAMTALLSLAIGTTWDDASAATRADFEPITFSLVGLTFGGIVMAIFGVNMVSG
                     EYSSGMIRTTLTATPKRGRVLAAKIVLIGAVAMLMSLVTTVGALAIGKAVLGSYDIPT
                     FSMGDSDVLRVIIASTLTAPLFPLIGATFAFLFRSTATPITLVLALIFLPAMFGALFP
                     DWWQENILSLLPGSAADSVMMGHLSDSRMYLDLLPAAIVVVVWLVITVGAAYVTLTRR
                     DA"
     gene            complement(1670..2638)
                     /locus_tag="VFV93_08620"
     CDS             complement(1670..2638)
                     /locus_tag="VFV93_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5165443.1"
                     /translation="MIEVRDLTKYYGSVKAVDGLSFDIKPGIVTGFLGPNGAGKSTTM
                     RMILGLDNPTSGAVTINGVAYRDLPAPTRVIGALLDAKEIFGRRSAERHLQWLAQAGG
                     IPKQRVAEVLDTVGLTSVAGHSINGFSLGMYQRLGIAAALLGDPEILLFDEPVNGLDP
                     EGILWIRNLMRRLASEGRTIFVSSHLMHEMEETADHLIVIGRGRLIADTSMTDFIQRS
                     THSHVRVVSPQAAELAPRLDAAGATITVADDGALIVTGIEAAQIGDIAASHGFRLHEL
                     SPQRARLEAAFMETTRDSVQYQAGQQEAVAAIFADAGETPAEQEVG"
     gene            complement(2713..3474)
                     /locus_tag="VFV93_08625"
     CDS             complement(2713..3474)
                     /locus_tag="VFV93_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165444.1"
                     /translation="MIAQSRTHAQRELPPPNWTFPRKMLEAHLWMVGLFWLALLVLVA
                     AIAVGNAIWASVNGSFWDDAVSSLPQWAIAVGSGYLAYNFLPAMIALGRTRREFGRQA
                     LAYSIFFSVVLALLVVVGFLLEKGLYAVMDWPQGIDNGHIYSTSSQIHLIFAEYLIRF
                     LVWSAAGFAIGAGFYRSEGAGGIALALGWIPVAVTEFLIGTQTGSPIVLLQLVWEPPS
                     LPALVVIVVGILSWLAAGALSWRVICDIPIRSKAS"
     gene            complement(3471..>4147)
                     /locus_tag="VFV93_08630"
     CDS             complement(3471..>4147)
                     /locus_tag="VFV93_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165445.1"
                     /translation="NPAITEQICLIRESVDVFERSAAIESVLGFTSAIRPGWDAEYAG
                     RLLDAFELDPRQKVKDLSRGKQSALGAILGLASRAPLTMFDETYLGMDAPSRYRFYDE
                     LLADYIAHPRTMIVSTHLIEEVARIFEEVVIIDEGRLVLHEPVDTLTARGATVTGPGD
                     AVDALAAGMTVLNERQLGRTKAVMVYGQLTDEQARQAQAAGLDLEPIALQDLFVYLTD
                     RKGVAR"
BASE COUNT          751 a         1386 c         1349 g          661 t
ORIGIN      
        1 cggaaacgac cagcttacgc atatctgtcc tccttgaaga cacaacgaac gacggccatc
       61 atcgccgcca cactgaatag tcgttcgtcg cggcccgaag tcgacactgg cgtcgcatga
      121 tacatcgctt cgtcgtcgcg aagttgagac gtaggggccg tgtcttgtgc cggccctgtt
      181 ccccacccga cgcgttcggg catccgagca gggccggcac aaggcacggc ccctacttat
      241 tcggggctga acggggcgat caggccgctg gcgtcgaact cctcgatggt catgccgtag
      301 gcgatgtagt cgtagtggcg tccgctgatg tagacgaggc ggcgcaggcg tccctcctgc
      361 tggaagccga gacgctcgaa caggcgcagc gagggctggt tgatatcgac gatgaacgcc
      421 gtcaccttct gatagcgcag ctcgtggaag tagtagcgca gcagcagcgc gatcgcctcg
      481 cgcgcgtagc ccctacgctg gtgctcgggg cggatcgcga tgccaagctc gaagatcccc
      541 gtccgccggt cgcagtcgtg ggccgcgacc gagccgacgt ggacaccctc cagcgtctcg
      601 acttcgaggt ggacgttgtc gccttccggc tcactggccg ccttctgctc ggcccagcgg
      661 cgggtgccct cgcgcgagcg gggaaggtga acgcgatcga gcagccgctg ggatgtgctg
      721 tcctggtccc atgcgtggtg gatctgccag tcgtccggct cgatggcgcg tagccggacg
      781 cgggaaccct cccagtagct cgccaatcgc gccctccctc ccccggtcgt ctcaggcgtc
      841 tcgacgcgtc agtgtgacat aggccgcacc gaccgtgatg accagccaga cgacgacgac
      901 gatcgcggcc gggagcagat ccagatacat gcgcgagtcg gacagatggc ccatcatgac
      961 ggagtcagcc gccgagccgg gcagcaggct gaggatgttc tcctgccacc agtccgggaa
     1021 gagcgcgccg aacatggccg gcaggaagat caacgccagc acgagcgtga tcggcgtcgc
     1081 cgtactccgg aacaggaacg cgaaggttgc gccaatcagc gggaagagcg gcgccgtcaa
     1141 cgtcgatgcg atgatgacac gcagcacgtc ggaatcgccc atcgagaagg tcgggatgtc
     1201 gtacgatccc agcacagcct tgccgatcgc cagcgcgccg acggtcgtca ccagactcat
     1261 cagcatcgcg acggcaccaa tcagcacgat cttcgcggcc aggacacgcc cgcgcttcgg
     1321 cgttgccgtc agcgtcgtcc ggatcatgcc gctcgaatac tcgcccgaga ccatgttgac
     1381 gccgaagatg gccatcacga tcccgccgaa ggtcagtccg accagcgaga aggtgatcgg
     1441 ctcgaaatcg gcccgcgtag ccgccgaagc gtcgtcccag gtggtgccga tcgccagcga
     1501 gagcagcgcc gtcatcgcga acgagagcac cagcgccagc ccgagcatca cataggtcga
     1561 gcgggtcgag aacatcttgg tccactcgga ggccagcgtc gcgccgaacc cgaccgggga
     1621 gtattcgcgc gtcggtgcgc ggttgagtgt ggttgcctgg gtagccatct tagccaacct
     1681 cctgttcggc tggggtctcg ccggcgtcag cgaagatggc ggcaaccgcc tcctgctggc
     1741 cggcctggta ctggacgctg tcgcgggttg tctccatgaa ggcggcttcg agccgtgccc
     1801 gctgcggtga gagctcgtgc agccggaagc cgtgcgaggc ggcgatgtcg ccgatctggg
     1861 ccgcttcgat gccggtcacg atcagcgcgc cgtcgtcggc aacggtgatc gtcgcgccag
     1921 ccgcgtcgag gcgtggcgcc agctcggccg cctgcggcga gacgacacgg acgtggctat
     1981 gggtgctgcg ctgaatgaag tcggtcatgc tcgtgtcggc gatcagccgc ccgcgcccga
     2041 tcacgatcag gtggtcggcc gtctcctcca tctcgtgcat caggtggctg gagacgaaga
     2101 tcgtccgacc ctcggaggcc agccgccgca tcaggttccg aatccagagg atgccctccg
     2161 ggtcgaggcc gttgaccggc tcgtcgaaga gcaggatctc cggatcgccc agcagcgcgg
     2221 ccgcgatgcc gagccgctgg tacatgccga gtgagaagcc gttgatgctg tgcccggcga
     2281 cactggtcag cccgaccgtg tccagaacct cggccacgcg ctgcttcgga ataccgcccg
     2341 cctgtgccag ccattgcagg tgccgctcgg ccgagcgccg gccgaagatc tccttggcgt
     2401 ccagcagcgc gccgatgacc cgcgtcgggg ccggcagatc gcgataggca acgccgttga
     2461 tcgtcaccgc accggaggtc gggttgtcga gcccgaggat catccgcatc gtcgttgatt
     2521 taccggcgcc attcgggccg aggaagccgg tgacgatgcc cggcttgata tcgaacgaga
     2581 gtccatcgac ggccttgacg ctgccgtagt atttggtgag atcacgtact tcgatcacgg
     2641 atggtgcgcc ttccttagag atgaatgatt cctaccgggc tccgcttcgt tccgcgcaag
     2701 gcagatgccg tcctacgaag ccttgctgcg gatcgggatg tcgcagatga cacgccagct
     2761 aagcgcgcct gcggccagcc aggaaagaat gccgacgacg ataacgacga gcgccggcag
     2821 gctcggtggc tcccagacca actgaagcag gacgatcggc gagccggtct gcgtgccgat
     2881 caagaactcg gtcacggcga ctgggatcca gcccagcgcc agcgcgattc cgccggcccc
     2941 ctcagagcgg tagaatccgg ccccgatcgc gaagccggca gccgaccaga ccaggaaacg
     3001 aatcaggtac tccgcaaaaa tcagatggat ctggctgctg gtcgagtaga tgtgcccgtt
     3061 gtcgatcccc tgcggccagt ccatcaccgc atagagcccc ttttccagca ggaacccgac
     3121 gacgaccagc agcgccagta ccaccgagaa gaagatcgag taggccagcg cctgacggcc
     3181 gaattcccgg cgggtccggc caagcgcgat catcgccggc agaaagttgt acgccaggta
     3241 gccgctgccg acggcgatcg cccactgcgg gagcgagctg acggcatcgt cccagaagct
     3301 gccgttgact gaggcccaga tggcattccc aaccgcgatc gcggcgacga gcaccagcag
     3361 cgccagccag aacagtccca ccatccacag atgcgcttcc agcatcttgc gcgggaaggt
     3421 ccagttgggc ggcggcagct ctcgttgcgc gtgcgtgcgt gactgcgcaa tcatcgcgcg
     3481 acccccttcc ggtcggtgag atagacgaac aggtcctgca gcgcgatcgg ctccaggtcg
     3541 agaccggccg cctgcgcctg ccgcgcctgc tcgtcagtaa gctgcccgta gaccatgacc
     3601 gccttcgtcc gtccaagctg ccgttcgttg aggaccgtca tcccggccgc caacgcgtca
     3661 accgcgtcgc ccggcccggt cacggtcgcg ccgcgcgccg tcaatgtgtc gaccggctca
     3721 tgcagcacca gccggccctc gtcgatgatg acgacctcct cgaagatccg cgcgacctcc
     3781 tcgatcagat gagtcgagac gatcatcgtt cgcggatggg cgatatagtc ggccagcaac
     3841 tcgtcgtaga agcggtagcg cgagggcgcg tccatcccga ggtaggtctc atcgaacatc
     3901 gtcagcggcg cccgcgaagc cagcccgagg atcgccccca gcgccgattg cttgccgcgc
     3961 gagagatcct tgaccttctg gcgcggatcg agctcgaacg cgtccagcag ccggccggcg
     4021 tactccgcgt cccagccggg gcggatcgcc gacgtaaagc cgaggacgga ttcaatcgcc
     4081 gccgaccgct cgaagacatc gacactctcg cggatgaggc agatctgctc agtgatggcc
     4141 gggttct
//
LOCUS       DASUGJ010000393         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1728840, whole genome shotgun sequence.
ACCESSION   DASUGJ010000393 DASUGJ010000000
VERSION     DASUGJ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728840"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VFV93_08635"
     CDS             <1..739
                     /locus_tag="VFV93_08635"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5165446.1"
                     /translation="MTFDEVEAVHDVHLTVGRGELVALVGPSGCGKSTLLAAIAGLLQ
                     PDEGEIWLDGSPAPNRLGNVALMPQRDALLPWRSVLDNAVLGLEVAGVNKREARERAR
                     ALLPRFGLAGFGEHYPAALSGGMRQRAAFLRTVLAERDVLLLDEPFGALDALTRRSMQ
                     EWLLDLWDELGQAILLVTHDVEEALLLADRVAVMTARPGRIKLVEPVHLPRPRTPEMI
                     SDPEFVAQKAALLATLHDEVALVGSAS"
     gene            736..1581
                     /locus_tag="VFV93_08640"
     CDS             736..1581
                     /locus_tag="VFV93_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5165447.1"
                     /translation="MTGLTRRRSLPEADRKPPRPAAARRPDWTVVLGWLVPLLALALG
                     LLAWEVIVRLRDTPTWFIPKPTEIAREMVESRRLIWRHTWTTLQEMLVGLVLAFVLGV
                     LLAVAISASRLVERAVYPAVVASQAIPIIALAPILLIWFGYGMTPKVIVVVLTCFFPI
                     VVSMVDGLRAADTDALALLRSMGASRWQLLRIVRVPAALPALFSGTRIAAAWSVIGAL
                     VGEWVGASSGLGYLMTRSASQFQTPRLYAAVVVAASLGILLFALVALAERVMLPWRRV
                     DRRGG"
     gene            1672..2811
                     /locus_tag="VFV93_08645"
     CDS             1672..2811
                     /locus_tag="VFV93_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5165448.1"
                     /translation="MKRPFRIIALLLAAAVLMSFVAACGDDDDDGDTPTATAATGTDA
                     PATAAASPSAEASPTAATEASPSMAGGSGEMTDISLALDWYPWGNHTGIYMALENGYF
                     EDEGLNVDVHVPADPTTALQLVATGDDDFTISYQADVLYARAQGLGIKSVAALVQHPL
                     NTIMALESSGIKEPADLKGKTVGITGVPSDEAMLGAVLEDAGLSLDDIKTVNVGFDLM
                     PSLLGGSVDAIIGGYFVHESILAEQQGKPVVAMNVHDYGVPDYYELLMVTSDTFADEH
                     PDAVEGFIRALQRGYAAAAADPDAAVDALVAAYPETAEDVERQGISLIVPLWTDNGSV
                     PWGTQTEERWQSYEEWLRDNGLLEKDIDVNDAFTNEFVEKANTAP"
     gene            2906..3310
                     /locus_tag="VFV93_08650"
     CDS             2906..3310
                     /locus_tag="VFV93_08650"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HEX5165449.1"
                     /translation="MTRLIEIEGIGPAHAARLEGVGVTSIESLLKTAGSAAGRKQLAA
                     KADIDESKLLEWVNHADLMRIKGVGSEFSDLLEAAGVDSVPELANRNPDNLHAKMLET
                     NEAKKLVRRVPTLEETRRWVTEAKTMDRAVTH"
     gene            complement(3386..>4453)
                     /locus_tag="VFV93_08655"
     CDS             complement(3386..>4453)
                     /locus_tag="VFV93_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX5165450.1"
                     /translation="EIRLIYWAGGNLFHQHQQLGKLRRGLAKVDTIVVHDPFWTAAAR
                     HADIVIPSTVTLERNDIGCSQNDRWLAAMQAAIPTHQQARNDYDSFSDIAAALGIGEA
                     FTEGRDEMGWLRHMYETWRADVAAHGHQFPDFDSFWAEGYVELPFDESNVLFAGFRAD
                     PTGAPLTTPSGKIELFSKTIDSFGYDDCLGHPAWFPRREWLGNERAERYPLLMIANNP
                     KTRLHSQLDIGDYSQSSKIQGREPIRIRPDDAARRGISDGDIVRVFNDRGGFLAGVII
                     SDELRPSVVQISTGAWYDPLDPADPDSICVHGNPNALTLDIGTSSLAQGCSGQHSLVQ
                     IERWDGPLPKISVLGPPNLRT"
BASE COUNT          710 a         1379 c         1589 g          775 t
ORIGIN      
        1 gatgaccttc gacgaggtcg aggccgtcca cgatgtccac ctcacggtcg ggcgcggcga
       61 actggtcgcg ttggtcgggc catcgggctg cggcaagagc acgctgctgg cggccatcgc
      121 cgggctgctt cagccggacg aaggcgagat ctggctggac ggttcccctg cccccaaccg
      181 gcttggaaac gtcgcgctga tgccgcagcg ggatgcgcta ctgccgtggc gatcggtcct
      241 ggacaatgcc gtcctcggtc tggaggtggc cggtgtcaac aagcgcgagg cgcgcgagcg
      301 ggcgcgggcg ctgctgccgc gcttcgggct ggccggcttc ggcgagcact acccggccgc
      361 cctctccggc gggatgcgcc agcgggcggc cttcctgcgg acggtcctgg ccgagcgcga
      421 tgtcctgctg ctggacgagc cattcggcgc gctggatgct ctgacgcgcc gctcgatgca
      481 ggagtggctg ctcgatctct gggacgagct gggccaggcg attctgctgg tgacccacga
      541 tgtcgaggag gcgttgctgc tggccgaccg ggtggccgtc atgacggccc ggccgggacg
      601 gatcaagctc gtcgagccgg tccacctgcc gcgcccgagg acgccggaga tgatcagcga
      661 cccggagttc gttgctcaga aggcggcgct gctggcgacg ctgcacgacg aggtcgcgct
      721 ggttgggagc gcgtcgtgac cggcctgaca cgccgccgct cgctgccgga ggccgaccgc
      781 aagccgccgc gcccagccgc cgcacgccgc cctgactgga ccgttgtcct cggctggctc
      841 gtgccgctgc tggcgctggc gctcggcctg ctggcctggg aggtgatcgt ccggttgcgc
      901 gacacgccga cctggttcat ccccaagccg accgagatcg ccagggagat ggtcgagtca
      961 cgccggctga tctggcggca cacctggacg acgctgcagg agatgctggt cgggctggtg
     1021 ctggccttcg tccttggcgt cctgctggcg gtcgccatct cggcctcgcg gctggtcgaa
     1081 cgggccgtct acccggccgt cgtcgccagc caggcgatcc cgatcatcgc gctcgccccg
     1141 atcctgctga tctggttcgg ctacgggatg accccgaagg tgatcgtcgt cgtcctgacc
     1201 tgcttcttcc caatcgtcgt cagcatggtc gacggcctgc gggcagccga caccgacgcg
     1261 ctggcgctgc tgcgttcaat gggagccagc cgctggcagt tgctgcggat cgtgcgcgtt
     1321 ccggccgcgc tgccggccct cttctccggc acccggatcg ccgccgcctg gtcggtgatc
     1381 ggggcgctgg tcggcgagtg ggttggcgca tccagcggtc tcggctacct gatgacccgc
     1441 tcggcgtcgc agttccagac cccgcgcctc tacgctgccg tcgtcgttgc cgcgtcactg
     1501 ggcattctcc tgttcgcgct ggtggcgctg gccgagcggg tgatgttgcc gtggcgtcgg
     1561 gtggatcggc ggggtgggtg attggtgatg gctcgatggg ctggggagga gtttgcgaca
     1621 gatgctggct ttgatgagga aacgaaactg atggttgagg aggagacacc catgaaacga
     1681 ccattccgca tcatcgcgtt gctgctggcg gcggcggtgc tgatgtcgtt cgtcgcggcc
     1741 tgtggcgacg atgacgacga cggcgacacg ccgacagcga cggcggcaac cggaacggac
     1801 gcgccggcaa cggctgcggc cagtccctcc gccgaggcga gcccaacggc ggctaccgaa
     1861 gccagcccgt cgatggcagg cggtagcggt gagatgaccg acatctcgct ggcgctagac
     1921 tggtacccgt ggggcaatca caccgggatc tacatggcgc tggagaatgg ctactttgag
     1981 gatgaggggc tcaacgtcga cgttcacgtg ccggccgacc cgacgacggc actgcagctg
     2041 gtcgcgactg gtgacgacga cttcacgatc tcgtatcagg ccgatgtgct ctatgcgcgt
     2101 gcgcaggggc tgggcatcaa gtcggtcgca gcactggtcc agcacccgct gaacacgatc
     2161 atggccctcg agtcatccgg catcaaagag ccggcagatc tgaaggggaa gacagttggc
     2221 atcaccggcg tcccgagcga cgaggcgatg ctgggggcgg tcctggagga cgccgggctg
     2281 tcgctggacg acatcaagac cgtcaacgtc ggcttcgacc tgatgccgtc gctgctgggc
     2341 ggcagtgtcg atgcgatcat cggcggctat ttcgtccatg agtcgatcct ggccgagcag
     2401 cagggcaagc cggtcgtcgc gatgaacgtg cacgactacg gcgtgccgga ctactacgag
     2461 ctgctgatgg tgacgagcga cacgttcgcc gacgagcacc ccgacgccgt cgagggcttc
     2521 atccgcgccc tccagcgcgg ctacgcagcg gcggctgccg atccagacgc ggccgtcgat
     2581 gcgctggtgg cggcctatcc ggagaccgcc gaagacgtcg aacgacaggg gatcagcctg
     2641 atcgtgccgc tctggaccga caacggcagt gtcccgtggg gtacccagac cgaggaacgc
     2701 tggcagtcat acgaggagtg gctacgcgac aacggtctgc tggagaagga tatcgacgtc
     2761 aacgacgcct ttacgaacga gttcgtcgaa aaggcgaata cagccccgta gatcgggcag
     2821 cgctgccagg cgaaggccgt ggtacgtccg agaattcgcg cgtatagttg aagcatggca
     2881 tcggagcaat cgaaaggggc ccaccatgac taggctgatc gagattgagg gcatcgggcc
     2941 agcgcatgcc gcgcgacttg agggagttgg agtgacgagt atcgagtcgc ttctcaagac
     3001 ggcgggatca gcggccggcc gcaagcaact ggcggcgaag gctgacattg acgagagcaa
     3061 gctgcttgag tgggtcaatc acgccgatct gatgcggatc aagggcgtcg ggtcggagtt
     3121 ctctgacctg cttgaagcgg ccggcgttga tagcgtccct gagctcgcca atcgcaaccc
     3181 tgacaatctc catgcgaaga tgctcgagac caacgaggcg aagaagttgg tccggcgtgt
     3241 gccgacgttg gaggagacca ggcgctgggt cactgaggcg aagacaatgg accgggccgt
     3301 tacgcactga cgccgattat attgacggaa tgcaaccggc gggccgcacg gatgatgtgg
     3361 cccgccggtt tgttgttggc gtgcattacg tccgcaggtt cggcggcccg agcacactga
     3421 tcttcggcag cggaccgtcc cagcgttcga tctggaccag cgagtgctgg ccggagcagc
     3481 cctgggcgag cgacgaggta ccgatgtcga gtgtcagtgc gttcgggttg ccgtggacgc
     3541 agatgctgtc cggatcggcc gggtcgagcg ggtcgtacca ggcgccggtc gagatctgga
     3601 cgacggatgg ccgcaactcg tcgctgatga tgacaccggc caggaagccg ccgcggtcat
     3661 tgaagacgcg gacgatgtcg ccgtcgctga tacctcgcct ggcggcgtcg tcgggacgta
     3721 tccgaatcgg ctctcgcccc tggatcttcg atgactggct gtagtcgccg atgtcgagct
     3781 ggctgtggag gcgggtcttc ggattgttag cgatcatcag tagcgggtac ctttcggctc
     3841 gctcgttgcc cagccattcg cggcgcggga accatgccgg atgtcccagg cagtcgtcgt
     3901 agccgaagct gtcgatcgtc ttcgagaaca gctcgatctt gccggatggc gtcgtcagtg
     3961 gcgcgcctgt cggatcagcg cggaagccgg cgaagaggac gttgctctcg tcgaacggca
     4021 gctcgacgta gccctcggcc cagaagctgt cgaagtcggg gaactggtgg ccatgggcgg
     4081 cgacatccgc ccgccacgtc tcgtacatgt gccgcagcca gcccatctcg tcgcggccct
     4141 cggtgaacgc ctcgccgatg ccgagtgcgg cagcgatgtc agagaagctg tcgtaatcgt
     4201 tgcgggcctg ctgatgggtc gggatagcgg cctgcatggc agccagccat ctgtcgttct
     4261 gcgagcagcc gatgtcgttg cgctccagcg tcaccgtgct ggggatgacg atgtcggcgt
     4321 ggcgggcggc tgccgtccag aacgggtcgt ggacgacgat cgtatcgacc ttcgccagcc
     4381 cgcgccgcag cttgccgagc tgctggtgct ggtggaagag gttgccaccg gcccagtaga
     4441 tcagccggat ctc
//
LOCUS       DASUGJ010000394         3481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1033878, whole genome shotgun sequence.
ACCESSION   DASUGJ010000394 DASUGJ010000000
VERSION     DASUGJ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033878"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1213
                     /locus_tag="VFV93_08660"
     CDS             92..1213
                     /locus_tag="VFV93_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5165451.1"
                     /translation="MSLVAKDQFIGGDDRAYLLTAGDGLVPQGVLDATRRYYENKALG
                     PVGYQRHGIVDRACRTKLAALMDGASADDIALLGSTSEGVNAIFALIDWQPGDNIVMP
                     VSALEFPSTVLPAVRRERIGVETRTVDHDDWIIAPEAIAAAVDDRTRLVFLSHVSYRA
                     GHRFDLAAVGEAIRRRNPETIFAVDATQSLGVVPVQADACDFLVVTSCKWLLAPHGIG
                     VFYWNRARRPDTEPAGIGWYSVVDDLQIPYELKPDAGRFELGGPNMVAIYGMEPGLDL
                     LLTTGVRAIERHVLRLGTHLLEGLLSLPLEIITPVDSELRAGIVAWLDPDPRATAAAL
                     AERGIAVTGSSGRIRAAIHLYNDESDVDRLVAAMREITG"
     gene            complement(1203..1550)
                     /locus_tag="VFV93_08665"
     CDS             complement(1203..1550)
                     /locus_tag="VFV93_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165452.1"
                     /translation="MTSQTIEIRAIWKERPSWFASGMMQWVAETVGQDGSQQVATSPA
                     FPLDSKLVVATEASDKSPYIVPDLGQMDDKKRRVVEEMLADFVRSLERQGWVQTGQGS
                     DWFSYQFQRGVSQ"
     gene            1650..2798
                     /locus_tag="VFV93_08670"
     CDS             1650..2798
                     /locus_tag="VFV93_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5165453.1"
                     /translation="MSDTTDMTTANDLDRLRAFVRDQVAPLARGWDEDERMPREHVQR
                     LAGAGLLGQTLSRDAGGAGRSPLDAARTIAEVARGCAVSARVLVDTNFGAVQIIDRWA
                     TDEIRTRYLPGVRSGEKLVAIAITEPGAGSAANELTTSATFDRDEVVLTGSKRWITGA
                     GERDLYLVFARFDDVPGAAGVGAVLVPAGTPGLRIGNREPTMGLRGLREGELHFEGCR
                     VPDSHLIVEPGPGAFGRLMAAYNGQRVGAASVSLGLGRGALDIAVDYAKERHQFGKPI
                     AEYQAIQLMLADMAIQLDAAELLIERAATTLDRHGFPARYETSVAKTFASEAGILATN
                     TAIQILGGNGYSRHYQVERMARDARMFTIGGGTSQMQRLAVAATLLAE"
     gene            2889..>3481
                     /locus_tag="VFV93_08675"
     CDS             2889..>3481
                     /locus_tag="VFV93_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5165454.1"
                     /translation="MESHETDATLVALARDGDRRAGHILLTRHYPMLVRRCRQRLRDP
                     ILAQDAAQEAAVQALLSLDRLRHPDKFGQWLSGIGVNTCRRWMQRRTHDEWSWDGLIG
                     GHDGIESVEADISPEELIAIGEVQTEVQLAVSELPPGQKAAVQLVYLDGLSHHEAALT
                     LNVAAGAIKTRLFKARRTLRRRLDTPWMEDYVTTSKQ"
BASE COUNT          628 a         1180 c         1125 g          548 t
ORIGIN      
        1 aggagagatc cttcggcgca cgcctcagga tgacaaacgg gggataacat acgcaccatc
       61 atcgatcatt gagtgattgg gagacggccg gatgagcctt gttgccaaag accagttcat
      121 cggcggtgac gaccgtgcct acctgctgac agccggcgac gggctagtgc cgcagggtgt
      181 gctcgacgcg acacggcgct attacgagaa caaagcgctc gggccggtcg gctatcagcg
      241 ccatggcatc gtcgatcgcg cctgccggac aaagctcgcc gcgctgatgg acggcgcgag
      301 tgcagacgac atcgcgctgt tgggcagcac ctccgaaggg gtcaacgcca tcttcgcgct
      361 gatcgactgg cagccaggcg acaacatcgt catgccggtc agcgcgctgg agtttccatc
      421 gaccgtcctg ccggccgtca ggcgcgagcg gatcggggtt gagacgcgaa ccgtcgatca
      481 cgacgactgg atcatcgcgc cggaggccat cgcggcagcc gtcgatgacc ggacacggct
      541 cgtcttcctc agccacgtct cgtaccgggc gggccaccgc ttcgacctgg ccgccgtcgg
      601 cgaggcgatt cgtcgccgca acccggagac gatcttcgcc gtcgacgcga cccagtcgct
      661 gggggtcgtc ccggtccagg cggacgcctg tgacttcctt gtcgttacct cgtgcaagtg
      721 gctgctggcg ccgcacggca tcggcgtctt ctactggaac cgggcacggc ggcccgatac
      781 cgagccggcc ggcatcggct ggtacagcgt cgttgacgac ctgcaaatcc catacgagct
      841 gaaacccgac gccggccggt tcgagctggg tggcccgaac atggttgcga tctacgggat
      901 ggagccggga ctcgacctgt tactgacgac cggcgtccgt gcgatcgagc ggcacgtcct
      961 gcgcctcggc acgcacttgc tggaagggct gttgagcctt ccgctggaga tcatcacgcc
     1021 ggtcgactcc gagttgcggg caggcatcgt cgcctggctg gaccccgatc cgcgtgcaac
     1081 ggctgccgcg ctggccgaac gcggcatcgc ggtgacgggc agctccggcc ggatcagggc
     1141 cgcgattcac ctctacaatg acgaatcgga cgtcgatcgg ctggtcgctg ccatgcggga
     1201 gattactggc tgacgccgcg ctggaactgg tagctgaacc agtcgctgcc ctgaccagtc
     1261 tggacccagc cctgccgttc gagggagcga acgaaatcgg ccagcatctc ctcgacgacg
     1321 cggcgcttct tgtcgtccat ctgaccgagg tcgggcacga tgtacgggct cttgtcgctg
     1381 gcctcggtcg ccacgaccag cttcgagtcg agtgggaacg cgggcgacgt cgcgacctgc
     1441 tgactaccgt cctgcccgac cgtctcggcc acccactgca tcatcccgga ggcaaaccag
     1501 ctcggacgct ccttccagat cgcccggatc tcgatcgtct gactggtcat ctgcctctcc
     1561 tcatcttccc ggtggcgtct gatgcgcgcg gcgaatgcac aatctatcat gcaatcagat
     1621 tcgcgtgacg gagcagcgga gagtgacaaa tgagcgacac gacagacatg acgaccgcga
     1681 acgacctcga ccgcctgcgc gccttcgttc gcgatcaggt cgcgcctctg gcacgcggtt
     1741 gggacgaaga cgagcgcatg ccacgcgagc acgtccagcg gctggccggc gcgggactgc
     1801 tggggcagac actcagtcgc gacgccggtg gcgccgggcg cagcccgctc gacgcggctc
     1861 ggacgatcgc cgaggtggcg cgcggttgtg ccgtcagcgc ccgggtactg gtcgacacga
     1921 acttcggtgc ggtccagatc atcgaccgct gggccaccga cgagatacgc acgcgctacc
     1981 tgccgggcgt ccgcagcggt gagaagctgg tcgcgatcgc catcaccgag cccggcgcgg
     2041 gttcagccgc caacgagctg acgaccagtg cgaccttcga ccgcgacgag gtcgtcctga
     2101 ccggcagcaa gcgctggatc accggcgccg gtgagcgcga tctctacctc gtcttcgctc
     2161 gcttcgatga cgtaccaggc gcggccggag tcggcgccgt cctcgtcccg gccgggacac
     2221 ccggcctgcg gatcggcaac cgcgagccga cgatggggct gcgcggattg cgcgagggtg
     2281 aactgcactt cgagggttgc cgcgtgccgg actcgcacct gatcgtcgag cctggccccg
     2341 gcgcgttcgg ccggctgatg gccgcctaca acggccagcg ggtcggcgcg gcctcggtct
     2401 cgctcggcct cgggcgcggc gcgctcgaca tcgcagtcga ttatgcgaag gaacggcacc
     2461 agttcggcaa accgatcgcc gagtaccagg cgatccagct catgctagcc gacatggcga
     2521 tccagctcga cgccgccgag ttgctgatcg aacgggccgc caccaccctc gaccggcacg
     2581 gcttccccgc ccgctacgag acgagcgtcg ccaagacctt cgcctccgaa gccggcatcc
     2641 tcgcaaccaa cactgccatc cagatcctcg gcggcaacgg ctactcacgc cactaccagg
     2701 tcgaacgaat ggcccgcgac gcccgcatgt tcacgatcgg cggcggcacc agccagatgc
     2761 aacgcctcgc cgtcgccgca acgttgcttg ccgaatgatc aacgccatcc ctacaacagg
     2821 gtaaccatct gacctcggat cttgactatt caacagatgg atcgagcacg accggtaatg
     2881 aggcaggttt ggaatcgcac gaaaccgacg cgacactcgt ggctctcgcg agagacggcg
     2941 accgccgcgc cggccatatc ctgctgacgc gccactaccc gatgctcgtg cggcgctgcc
     3001 ggcaacggct acgtgacccg atcctcgccc aagatgcggc tcaggaggcg gcagtacagg
     3061 cgctgctgag cctcgatcgc ctgcgccatc ccgacaagtt cggtcagtgg ctctcaggaa
     3121 tcggcgtcaa cacatgtcgc cggtggatgc agcgtcgaac gcatgatgag tggtcatggg
     3181 acgggctgat cggagggcat gacggcatcg agtcagtaga agctgacatc agtccggaag
     3241 aactgatcgc aatcggcgag gtccagaccg aggtgcaact agcagtcagc gaactgccac
     3301 cgggacagaa agccgccgtc caactcgtct acctcgatgg actgagccac catgaggcag
     3361 cactgacgct caacgtcgcg gcaggcgcga tcaagacgcg gctcttcaag gcccgccgga
     3421 cgctccggcg acgcctcgac acaccgtgga tggaggacta cgtgacgacc tcgaagcagg
     3481 a
//
LOCUS       DASUGJ010000395         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1866683, whole genome shotgun sequence.
ACCESSION   DASUGJ010000395 DASUGJ010000000
VERSION     DASUGJ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866683"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFV93_08680"
     CDS             complement(<1..725)
                     /locus_tag="VFV93_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165455.1"
                     /translation="MTDLGLVRAVLGVGLAGVMLEKWSTTVAIEIALPYRSDWREMSN
                     ILDYCKGKGADRSSLEARFGGGESLRETLNALEQLGLIERSESGDTRLTGQGEALAYA
                     PDQARLRERLIESMLAYPPYRFPLERALANGPAVLDGPWIEHIWQVDMRLGQPRNRVE
                     EARTFFYRLADEAGLGVYRRGVRGQTTRLELASDFAERLGEVIERQAAGSRPSVGRAA
                     SREAAPAVEPVASVMMPVAAPDRS"
     gene            1448..>2500
                     /locus_tag="VFV93_08685"
     CDS             1448..>2500
                     /locus_tag="VFV93_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165456.1"
                     /translation="MGQPSSTDAGAAAGDEGVTNQERSGLDARRRGWFWHWNNVVTQY
                     APLIGLKGVGLLNSYTVWTDRRDESPHRGYAFPSQQSEADFYGEDRSELITINKILVA
                     LDLIEIRKEMLTRVDDRGRRWRVPHNLYRVKDRPEGVDLRAIDVLRVAQLAQRDDAVF
                     RYVRRVFSERFRPIDGDNVWHAILTELRNDATWRELQDRTRAIEKRASARTRAGHQSR
                     AGGARATEATPSPAPETTEAGEADDVLLSDTQQSGSSASLPATSVDASNTGSNKSTET
                     DVEPSNTGSVVDVAASNTGSGRSGPSSAAAGNDGPAGVVGPSNTTYDQSLSTTTTPTT
                     RLTDAGWDGRRSSADHP"
BASE COUNT          482 a          835 c          770 g          413 t
ORIGIN      
        1 gatcggtccg gcgctgcgac aggcatcatc acggaagcca ccggctcgac agctggcgcg
       61 gcctcgcgac tggcggcgcg cccgacggac ggccgcgacc cggctgcctg ccgctcgatg
      121 acctccccga gccgctcggc aaagtcggac gccagttcca gccgcgtcgt ctggccgcga
      181 acgccgcgcc ggtagacgcc gagtcccgcc tcatcagcca gccggtagaa gaaggtccgt
      241 gcttcctcga cccggttgcg cggctggccc agtcgcatgt cgacctgcca gatatgctcg
      301 atccacggcc cgtcgagaac ggccggtccg ttcgccagcg cgcgttcgag cgggaaccga
      361 tagggcgggt atgcgagcat cgactcgatc agtcgctcgc gcaagcgcgc ctggtccggc
      421 gcataggcga gcgcctcacc ctggccggtc agccgggtat cgccactctc gctgcgttcg
      481 atcaggccaa gctgctccag cgcgttcagc gtctcgcgca gactctcgcc cccaccgaag
      541 cgagcctcaa gcgacgagcg atcggcgccc ttgcccttgc agtagtcgag aatgttcgac
      601 atctcgcgcc agtcactccg gtagggcagc gcgatctcga tcgccacggt cgtactccat
      661 ttctccagca tcaccccggc cagcccgacg ccgagaaccg ctcgaaccag cccgagatcc
      721 gtcacgcgcc tgccgagagc tacaacccga acgtcccgac ttcccgcgac acaggtccac
      781 gattgctacg aagaagacac tgcccgcaca cccgcgattc aacacgctac gcgcctggtc
      841 gtttcaccaa gcgtactcgc cacgtgcata tacgtcaagc catgtgtaac gagaggtggg
      901 cattcgtcct attgctggtt gcgtgtgtag ttccgtctgc gtgacgaata gtccatctac
      961 gttaattcaa gcgggattgc ggttcttatc aagcattacc acacgcgcgg cgctacttga
     1021 gttgaggaat cgcgagtgtt attgtgtaga ctgtgtaccg aagggttctt tcggttccgt
     1081 gtcggcagcg tatcgagggc agatgactac ccgtcggaaa cgcgatcagc agccagctcc
     1141 cggccacggt gacgagactg caatgcgcgg ctcgcactcc gaagagttgg ccctggcgcg
     1201 gcatcgcgca tccgaccagc cgcgggccag cgcgcagcgg ttggaatccg accgctccct
     1261 cctaccggcc ggcgagggga tcgagcgcgg tagtagcgtc gttgacagac gaagagacgg
     1321 gcaggctcat gcatcggctg cgaacgctgg agggcgtcga tccgaccacg atcaggacgg
     1381 tgacgacagc gtgctggttc cagatcgaac gtccacgctg gagactgaca cagcttcccg
     1441 gacggctatg gggcagccat cgtcgacgga tgcaggagcc gctgccggag acgagggggt
     1501 cacgaatcag gagcgcagcg gcctggacgc tcgccggcgc ggctggttct ggcactggaa
     1561 caacgtcgtc acccagtacg cgccgctgat cggcctcaag ggcgtcggcc tgctcaactc
     1621 ctataccgtc tggaccgacc ggcgcgacga atcaccccac cgcggctatg ccttccccag
     1681 ccagcagtcg gaggccgact tctatggaga agatcgatcc gaactgatca cgatcaacaa
     1741 gatcctggtc gcgctcgacc tgatcgagat ccgcaaggag atgctgaccc gcgtcgatga
     1801 ccgcggtcgt cgctggcgcg tgccacataa cctctatcgc gtgaaagatc gccctgaagg
     1861 cgtggactta cgtgcgatag acgttctccg cgtggcacaa ctcgcccaac gggatgatgc
     1921 ggtcttccgc tatgtgcgcc gtgtcttctc agaacggttt cgcccgatcg acggcgacaa
     1981 cgtctggcac gccatcctga ccgagctacg caacgatgcg acctggcgtg agttgcagga
     2041 tcgtactcgg gccatcgaga agcgcgccag tgcccgcacc cgcgccggac accagagccg
     2101 ggcaggcggt gcacgagcga ctgaggcaac gccaagcccc gcgccggaga cgaccgaagc
     2161 gggcgaggcg gatgacgtgt tgttgtccga cacacaacaa agtggctcgt cagcatcgct
     2221 cccagcaacc agtgttgatg ccagcaacac tggttcgaac aagtcaacgg agaccgatgt
     2281 tgaaccatcc aacactggtt cggtggtcga tgttgccgcg tccaacactg gttcggggcg
     2341 ttccgggcca agcagtgctg ctgccggcaa cgatggtccg gcaggcgttg ttggtcccag
     2401 caacactacg tatgaccaaa gcctatctac aacaacgaca cccacaactc gcttgactga
     2461 cgcgggctgg gacggacgcc ggagcagcgc tgaccacccg
//
LOCUS       DASUGJ010000396         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_894736, whole genome shotgun sequence.
ACCESSION   DASUGJ010000396 DASUGJ010000000
VERSION     DASUGJ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894736"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..621
                     /locus_tag="VFV93_08690"
     CDS             142..621
                     /locus_tag="VFV93_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5165457.1"
                     /translation="MIPGDTRHSLRTSVLLTISLAVFLATAVGVVEDGNQFLPATVVA
                     AQAQPDTYRNVYDGWKWWHVYCYRCHGTDAIGTANAPNLSDPNSKLSQAEFHKIVSKG
                     IPDKGMQAWDKLLDDKQIDQIHLYVQARRDKVLPPGRPDEVGPNKGQWVPPNGWPRR"
     gene            1081..1503
                     /locus_tag="VFV93_08695"
     CDS             1081..1503
                     /locus_tag="VFV93_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165458.1"
                     /translation="METASGAPIAVRAWWQQGERTGYGFIYSKEQLAALNRSVASAEP
                     APIAPAADADAAAGEPIAEGPVSGPDVVDGPGLPADEAEFQVAQAQEPPAPAPVPAPE
                     PRQETTRETLPQTASPLALLLLGGIASASVGLRLFKRS"
     gene            1586..2515
                     /locus_tag="VFV93_08700"
     CDS             1586..2515
                     /locus_tag="VFV93_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5165459.1"
                     /translation="MRILVPLATLTVALIAPNITAQSHFSARSDLVVLNVAVTDSRGA
                     FVSGLPKDAFKVIDEGQPQKIEFFAEQDAPATIGLLIDASGSMLENRDRVIAGVTEFA
                     KASHPDDEFLPLIFNEQVTSVLSPGRFTSDPMELRDLLTTTLAARGRTAFHDALAEAV
                     EKVGRGRYERKVLIVLSDGGDNASRLSLDEAFDKVLASNVVIYSIALVDPLALDKNPK
                     ALRRLADGTGGLAFEPANVSVVSRAFQAIAHDIRSRYTVAYAPPDSVPEGHLRRVRVV
                     IESPGRTGFKVRTRTGYIPDSSPAVSAGRGVPR"
     gene            2512..>3022
                     /locus_tag="VFV93_08705"
     CDS             2512..>3022
                     /locus_tag="VFV93_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HEX5165460.1"
                     /translation="MIRRLFALLFVLVGLIVIVGAGFALFWSDELQRIYVPSLPSIER
                     HSGSQSAPGPEVGEGKPIGLIEIPRLGLSSVVLEGDDTAALLLGVGHLSDTPLPWHGG
                     NTVFAAHRDTFFRPLAHIRKNDVIRFSTADAGFEYVVTELKVVEPTDVEVLEPTTAAT
                     LTLITCFPFD"
BASE COUNT          595 a          898 c          939 g          590 t
ORIGIN      
        1 tatctcgatg ggcgtcaagc cgggcaacac ggcgctgaag actcagttgg aaaaagtgat
       61 cgacacacgg cgtgcagaga tcacgaagat cctgaacgac tacaacgttc cactcgtcca
      121 gaggaaggca ggccagaagc gatgatcccc ggagacacca gacactccct gcgcacgtcg
      181 gtattgctga ccatatccct tgcggtgttt ctcgcgaccg cggtcggcgt cgttgaagat
      241 ggcaatcagt ttctgccggc gaccgtcgtg gccgcgcagg cgcagccgga cacatatagg
      301 aacgtgtacg acggttggaa gtggtggcac gtctactgtt accgctgcca cggtaccgat
      361 gcgatcggta cggcaaacgc gcccaatctc agcgatccga acagcaaact aagccaggcc
      421 gagtttcata agatcgtgtc gaaggggatt cctgataagg gcatgcaagc gtgggacaag
      481 cttctcgacg acaaacagat cgaccagatt cacctgtacg tgcaggcgcg aagagataag
      541 gtgctgccac ccggacgtcc cgatgaagtg gggcctaaca agggacagtg ggtcccgccg
      601 aacggttggc ctcgtcgctg acctggcgtc ctgtacatgt ttcatttaca tgagccggct
      661 tgcggccacc tcgagtgcat gagaactcga gcatcctgac gtaagccgct ctccacgcga
      721 aggttcttcc cgctcgagtt ggaatcggca ggcatcttgc cattccatag gggcaggagg
      781 accagcatgt tgaagaggct tattaccact tccgtgttgg tggtgcttgt cggagcggca
      841 agtggcgcca acgcgttcac gaatacgtca gacaagcgaa cgtacttcac attcagccag
      901 ccagtggctc ttccgggagt aacgctcccg gctggaacgt acatgttccg tttggctgac
      961 gataccacca cgcgtaaggt cattcaggtt acaaacagag aggggacgca gagtttcgcg
     1021 atgctccaca cgatgcccgt gtatcggccg gacgctccgc gagatcccga agtccgattc
     1081 atggagactg cctcaggtgc gccaatcgct gtaagggcct ggtggcaaca gggtgaacga
     1141 actggctacg ggttcatcta ctcgaaggaa cagctcgcgg cgctgaaccg cagtgttgcg
     1201 tcggcagagc cggcgccaat tgcgccagca gcggacgcgg atgcagcagc cggtgagccg
     1261 attgccgaag gtccagtcag tggacccgac gtggtcgacg gacccggcct gccggccgat
     1321 gaagccgagt tccaggtagc acaggcgcag gagccgccag ctccagcacc agttccggcg
     1381 ccggagccga gacaagagac gacgcgagag acgctgcccc agacagccag cccattggcg
     1441 ctgctgttgc tcggtggcat cgcgagcgcc tcagtgggtc tgcgattgtt taaacggtcg
     1501 taaccagggc atggctgact cgtgaacacg acacgggggg agggatgctc cctcccctcg
     1561 ttgggatgtc ttgcagagga acaccatgcg catactcgtt ccactggcca cgctgacagt
     1621 cgccctcatc gcgccaaaca tcacggcgca aagtcatttc tccgcgcgat cggatctcgt
     1681 cgtcctcaac gtcgccgtga ccgattcccg tggcgcgttt gtcagcgggt tgccgaagga
     1741 cgcgtttaag gtcatcgacg agggacagcc acaaaagatc gaattcttcg cggaacagga
     1801 cgcgccagcc actatcggtc tgctcatcga tgccagcggc agcatgctcg agaaccgcga
     1861 tcgcgtgatc gccggcgtca ctgaatttgc gaaggccagt catccggacg atgagttctt
     1921 gccgctcatt ttcaacgagc aggtcacatc ggtgctctcg ccaggacgat tcacgagtga
     1981 tccgatggaa ctgcgtgatc tcctgaccac gacgctcgcc gctcgcggcc gtacggcatt
     2041 tcacgatgcg ctggcggaag ctgttgaaaa agtcgggcgc ggccgctatg agcgcaaggt
     2101 gctgatcgtc ttgagcgacg gtggcgacaa tgccagccgg ttgtcgctcg acgaagcctt
     2161 cgacaaagtt ctcgcatcga acgtggtgat ctattcgatt gcgctggtcg atccactcgc
     2221 actcgacaag aacccgaagg cgcttcgacg cctggctgat ggcactggtg gcctggcgtt
     2281 cgagccagcc aatgtgtcgg ttgtgagccg cgcgtttcag gccatcgctc acgacatccg
     2341 cagccgctac accgttgctt acgcgccacc ggattcggtt cctgagggtc atcttcggcg
     2401 ggtgcgcgtc gtgattgaat cgcccggccg gaccggtttt aaggttcgga cgcgcaccgg
     2461 ctacatcccc gactcgtctc cggccgtctc ggccggacgt ggggtgccgc gatgatccgt
     2521 cggctcttcg cgctgttgtt cgtcctcgtg gggctcatcg tgatcgtggg cgccgggttt
     2581 gcgctgttct ggtccgatga gctgcagcgg atctatgtac cgtcgttgcc cagcatcgaa
     2641 cgtcattcag gcagccagtc ggcgccgggg cctgaagtgg gggaaggcaa gccgatcggc
     2701 ctgatcgaga ttccgcgact tggtctctcg tcagttgtgc tcgagggcga cgataccgcg
     2761 gcactgttgc tgggcgtcgg ccatttgtcc gacacgccgt tgccgtggca cggtggcaac
     2821 accgtgtttg ctgcgcatcg cgacaccttc ttccgtccgc tggcgcacat ccgcaagaac
     2881 gacgtcatca ggttctcaac ggccgatgcc gggttcgagt acgtggtcac ggagttgaag
     2941 gtggtggagc cgaccgacgt tgaagtgctg gagcccacaa cggcggcaac attgacgcta
     3001 attacctgct ttccgttcga ct
//
LOCUS       DASUGJ010000397         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_339818, whole genome shotgun sequence.
ACCESSION   DASUGJ010000397 DASUGJ010000000
VERSION     DASUGJ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339818"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1079)
                     /locus_tag="VFV93_08710"
     CDS             complement(45..1079)
                     /locus_tag="VFV93_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX5165461.1"
                     /translation="MAHKILILSRKFRDGNREYVEEFLDRRNCVALDPDLTYPVSQDQ
                     LVEVVGEAEGIITGLEWISSRIIEAAPKLKVVSTGGVGFDHVDINAANEHGIAVGNCA
                     GCNNHSVSELAFGMMISLARNVGVHDKTIREGGWQPYDMLRMGSELWGKTLGVVGLGR
                     VGKSSALLGKAFGMKVIASDIAWDITFANEHGISLMPLDSLLQQADYVTLHTPLTNLT
                     RDLIDERAIDLMKHGAFLINTSRGPVVKESALVSGLASGKIAGAGLDVFRVEPHPDNP
                     YVEFPNVVLTPHIGGSTQEAFDRSLYLALVNVTNVFNGIEPHCQVNPGVSPHLEFQSR
                     VEMVRPPSSI"
     gene            1182..>2170
                     /locus_tag="VFV93_08715"
     CDS             1182..>2170
                     /locus_tag="VFV93_08715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165462.1"
                     /translation="MTETVAVRVEQHVSRRRKLTALAWICLAEFLGMSIWFSFSAIKP
                     ILADEWHISNTDAGYILSAFQLGYIASVAVVGILADRYNTRAVLAVSAALAGILNLGF
                     ALFADGLWSGIVLRLLVGAAMAGVYTPGIKIVAGWFAPHERGRAVGVFVGALVLGSAS
                     PYLLAPVATGIGWRALVIVTVAAAFVAAGIMWFIVEDPPVTTSTPFAFDPRLLKDRPL
                     RLMNLGYVAHMWELYAMWAWIGPYLIHVLNEQGRSSDAATDLGGQLAFVIIGIGALAC
                     AVAGFLGDRFGRTLTTSVLLVISAACSLTFGFMQTAPVALIALVGLVYGFSIVG"
BASE COUNT          319 a          688 c          732 g          431 t
ORIGIN      
        1 cggcacgaga ccggccccta tttgatggcg ggtccgaagg tcgcctaaat cgaggacggc
       61 ggtcggacca tttcgactct cgactggaac tccaggtgcg gcgagacgcc ggggttgacc
      121 tggcagtgcg gctcgatgcc gttgaagacg ttggtgacgt tgaccagcgc caggtagagc
      181 gagcggtcga acgcttcctg ggttgagccg ccgatgtgcg gcgtcaggac gacgttgggg
      241 aactcgacgt aggggttgtc cgggtgcggc tcgacccgga agacgtccag gccggcgccg
      301 gcgatcttgc cggaggcgag gccggagacg agcgccgatt ccttcacgac cggccctctg
      361 gaggtgttga tcaggaacgc gccgtgcttc atcaggtcga tcgcgcgctc gtcgatcagg
      421 tcgcgcgtca gattcgtcaa tggcgtgtgg agggtgacgt aatccgcctg ctggagcagg
      481 ctgtcgagcg gcatcagcga gataccgtgc tcgtttgcga aggtgatgtc ccaggcgatg
      541 tcggaggcga tgaccttcat cccgaacgcc ttaccgagta gcgctgacga cttcccgacg
      601 cggccgaggc cgacgacgcc gagcgtcttg ccccacagtt cggaccccat ccgcaacatg
      661 tcatacggct gccaaccgcc ctcgcgaatc gtcttgtcgt ggacgccgac gttgcgggcc
      721 agcgagatca tcatcccgaa cgcgagttcc gagaccgagt ggttgttgca gccggcgcag
      781 ttgccgacgg cgatcccgtg ctcgttggcg gcgttgatgt cgacatgatc gaagccgacg
      841 ccaccagtgc tgaccacctt gagcttcggc gccgcctcga tgattcgcga ggagatccac
      901 tccaggccgg tgataatgcc ttcggcttcg ccgacgacct caacgagctg gtcctgagag
      961 accgggtagg taagatcagg atcgagcgcg acgcagttgc gccggtcgag aaactcctcg
     1021 acgtactccc ggttcccgtc gcggaacttg cgggagagga tcaggatctt gtgcgccatc
     1081 gtgcgtgccc ttcactcctc gctgatggcc cggtgcgggc ctgtactctc gatgggcggc
     1141 attgtaacgc gcttcctggc tggtggcgct atcatgcgcg catgaccgaa actgtggccg
     1201 tccgcgtcga gcaacacgtg agccgccggc gcaagctgac ggcgctggcc tggatctgcc
     1261 tggccgagtt tcttggtatg tcgatctggt tctcgttctc ggcgatcaag ccgatcctgg
     1321 ccgacgagtg gcatatctcc aacaccgacg ccggttacat cctgtcggcg tttcagcttg
     1381 gttacatcgc gtcggtcgct gtcgttggca tcctcgccga tcgctacaac acgcgggcgg
     1441 tcctggccgt gtcggctgca ctggccggca tcctgaatct cggcttcgcc ctgttcgccg
     1501 acggactctg gagcgggatc gtgctgcggc tgctggtcgg cgcggcgatg gccggtgtct
     1561 acacgcccgg catcaagatc gtcgccggct ggttcgcgcc gcacgaacgg ggacgggcgg
     1621 tcggcgtctt cgtcggagcg ttggtactcg gttcggcgtc accctatctg ctggcgccgg
     1681 tcgcgaccgg catcggctgg cgcgcgctgg tgatcgtgac ggtcgctgcc gccttcgtcg
     1741 cggccgggat catgtggttc atcgtcgagg acccgcccgt cacgaccagc accccctttg
     1801 ccttcgaccc tcgcctgctg aaggatcgcc cgctgcggct gatgaacctc ggttacgtcg
     1861 cgcacatgtg ggaactgtat gcgatgtggg cctggattgg tccctacctg atccacgtgc
     1921 tgaacgagca gggccgctcg tcggatgcgg cgaccgacct cggcggccag ctcgcgttcg
     1981 tcatcatcgg catcggcgcg ctggcctgcg ccgtcgccgg gttcctcggc gaccggttcg
     2041 ggcgaacgct gacgaccagc gtcctgctcg tcatcagtgc ggcctgctcg ctgacgttcg
     2101 ggttcatgca gacggcgccg gttgcgttga tcgcactggt cggtctcgtc tacggcttct
     2161 cgatcgtcgg
//
LOCUS       DASUGJ010000398        11217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_479612, whole genome shotgun sequence.
ACCESSION   DASUGJ010000398 DASUGJ010000000
VERSION     DASUGJ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11217
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479612"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            743..2596
                     /locus_tag="VFV93_08720"
     CDS             743..2596
                     /locus_tag="VFV93_08720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5165463.1"
                     /translation="MPTRNDHWSREPGRWETSLQPEAWATIPTIGTPRPSPDGRTIAY
                     SRGYDGRLDIWTLDLATGRTMQLTDVARPQGADLSQRQASSIAWTPDSSTIVFASNQE
                     GKLWSVPAGGGPSKPIDEAAGNHHSPVISPDGTQVAYVAERGESVDIFVAAIDGRWVR
                     QISSHDNDEYVAEPSWSPDGRSLLYMQWPHYDMPWDEAAVVVADLESGERRIVAGGER
                     VVNNWPRWSQDGQSIVYVSDRGGEHPNLWEVRPDGSGMERLVEAPNDLLWPVVSPDGR
                     TVAFTKNEDNEGHVCIIGADGHVRQLTNEHGVHTELAWIDERRLVGVYQSPLAPPDLW
                     EITLDGERRQLTFSATGGVVGGDLVLPEVITWQSRDGMTIHGTLFSPPEIQPGKHPLI
                     VHIHGGPVGQANKTWIPWIQYLVQRGYVVLVPNYRGSKGYGRTFMEALYGDWGGGDLD
                     DNITGAEAVIARGAVDPTRVVATGGSAGGYSTLICMTRAPEVFKAGIARYGIANLATF
                     TDKTWIFERHYLAKLMGGTGGSRSDLYRERSPLTHVDNVRGPLLILQGEDDIVCHRSE
                     MDTMAAALEAAGKHVEYTVYPDEGHGWKHVSTIIDDARRSDDFLVRMVLNR"
     gene            complement(3110..3787)
                     /locus_tag="VFV93_08725"
     CDS             complement(3110..3787)
                     /locus_tag="VFV93_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5165464.1"
                     /translation="MAKRTYGQYCPVARTSEIIADRWTPLIIRELLAGIDGFNALHRS
                     LPGISRSVLVDRLQRLEDDGIIERYPGGAGRLTTYQLTPAGQDLRLVIDGMAAWGFRW
                     AFGEPRPEEQDPWLLLWWMRRRVNTNALPERRIVIQFDFSGGWTGSYWLLLESTDVTI
                     CLHHPGFEIDLLVNTDISALYRVWLGQLTLTTAQGAGLIQVDGPRSLAQPFLSEWFDW
                     NAFATVH"
     gene            3964..5388
                     /locus_tag="VFV93_08730"
     CDS             3964..5388
                     /locus_tag="VFV93_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5165465.1"
                     /translation="MQAQATTTDSGIDRSILDELIAAVRCPVLLPGSSDYDQARALHN
                     GLIERRPAVIVQCGGVADVVACVNVARERGLLLSVKGGGHNVAGNAVNDGGIVIDLAR
                     MTSVRVDPLARTARVEGGARWADVDRETQVFGLAVPGGVVSTTGVAGLTLHGGMGYLR
                     RKHGLSIDNLLSVDIVMADGQVRSVSAANDPDLFWAVRGAGSNFGVVTSFEFQLHPVG
                     PLVTLCAVFYAQADAERVVRAWRDVVTMAPDELSSFAVFWSVPAEPPLFPSELHGTPV
                     VIVNAVYAGGAEEGERAIQPLRELATPLIDLSGPLPYQEVQTLFDPFFPPGLLYYWKS
                     LNLDHLDDAVIDEVCRLAAERPSERSDLMLWCQGGAIGRVAAEATAFGRRDAPFMLLF
                     DSTWRNPEDSTRNIAWTRSAWSSMQQYSTGGGLYLNFPGLGEESAKLLRSAHGANYAR
                     LRALKATYDPTNLFRSNLNIPTTG"
     gene            5533..7938
                     /locus_tag="VFV93_08735"
     CDS             5533..7938
                     /locus_tag="VFV93_08735"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5165466.1"
                     /translation="MHSSLPADPPRPIHSPTPIAPGRVVAPVQSLPVPLTPLVGRGRE
                     VELAAGLLQTANVRLLTLSGPGGVGKTRLLLRLAEELAGVFPGGLTFVPLAPIDDPRL
                     LLPTIAGALGLRDDGGNDLTAQLAHALNGWPRLLLLDNFEQIVEAGPSLTELLAVCPA
                     LKIIVTSRVVLRVTGEQELVIPPLHVPGASRSRSLDEIATSEAIALFLLRATSVNPSF
                     TLTEENAPAIVEVCRRLDGLPLAIELAAARTKVLSPQALAERLGNRLQVLIGGPRDQP
                     ARLQTMRGAIAWSYDLLEPNEQELFRRLSVFPAGFDLDAAEQVAGSDLVLDGITSLVD
                     KSLLQQTNGHGAEPRFTMLETIRAFASEQLGASGEEHETRQRHAGWCQALVAAGAATM
                     RATPDQSALNRLEQEHDSLRVALGWLLDTGAVEDAARMVVGAWWFWFTRSHLRAGREW
                     SVRAFARLDRPPDLLWLRLNSVTGWFAEAVGEFETALAMHQRGLEVARELGDPESLGV
                     ALYALADVVDEQRETEQALTLFREAEAIFRELDAAPWLAVTLNSIGAIYREIGELDLA
                     IVLIEEGLALARERNHTWIIALSLGHLGRVYRMRGDLSRAIQLDRQTVRLWHEVGDWW
                     RMSRAINELGVAAQLTGHHDYAARLLGGSEAMREQYGAAFMLLLTSAYEQALTETQRH
                     LGDEGFTAAWDAGRSLSLEELLDLATNTPVTQVEARPRETSTAGLSAREMDVLRLLVA
                     GYSDRQIAEDLFISHRTAQGHVGSIFNKLGVNSRTAAATTAIRLGLVGTPEQQDAGGN
                     TLL"
     gene            8089..10440
                     /locus_tag="VFV93_08740"
     CDS             8089..10440
                     /locus_tag="VFV93_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5165467.1"
                     /translation="MATSMTPLLTTAGSSSGPRLPTSLTPLIGREQEIAALRAFVLGD
                     GTRLLTLTGPGGSGKTRLAQQLAIDLGNDFAAGSRFISLASVTEPEHLLQAIAVEMGI
                     REEANRSLADLVPMTLRQHELLIVLDNFEQLVEAAPVVSLLLGQCLRLAIIVTSRVPL
                     HVQGEQEYPVPPLALPGAQRAAPVEELASCAAIALFIARARSVDPGFVLDERNAQTVA
                     EICLRLDGLPLAIELAAARMKILSAEALLARLANRLRILIGGARDLPARQRTLRDTIS
                     WSYDLLSPDEQALFRRLAVFHGGFTLDGAAAVAGDIDALDGVGSLVDKSLLRRADAAG
                     MARFTMLETIREFALELLMTSDEAADVQRSHATWYAELAEDARTGIYSSEQRVWLDCL
                     EVEHDNLRAAMTFAAEHNDNALLLRFTAVLGDFWTMRGHFSEGHGWLKRAVMAGSANP
                     SPALAQTLSGAAIMSTVIGEPDAARQFAEDGLRVARLTGDGFSIGIALFCCADVAEEV
                     GDQNVAVDTYLQAAEVFRSLEEWVLLALTLANVGATCVSLHRYDEAIPPLQEALELST
                     THLFDLGIGMCSANLSRIEWARGDLVAARDHSRQSLQVWLVLGDQMRIVRLLERMAAI
                     AAADRQPVLATRLLSAAEARFDSFGVARTAGSVQAIDQAIKTIEPQLSREAFAGAWDA
                     GSALSIDEAVAEALAFELMTAPTAPTPATDRSLLSAREQEVVQLLVAGYSDRQIAESL
                     FISHRTAQGHVASIFNKLGVNSRTAAATTAIRLGIVSDGGPPA"
     gene            10576..10788
                     /locus_tag="VFV93_08745"
     CDS             10576..10788
                     /locus_tag="VFV93_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165468.1"
                     /translation="MQATMQATTDTTGLVAQGFTAEQIQRLEELRAVYPMIEMVENIE
                     QLRRIVFLKWLHEHRDDPADSIDTAA"
     gene            10868..11104
                     /locus_tag="VFV93_08750"
     CDS             10868..11104
                     /locus_tag="VFV93_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165469.1"
                     /translation="MMIQTVTHPACASATCPPVDHISTPHTVVAAEGSELAARLRSNG
                     WIARAGWRRYTAGRPAAWLVRFECPSACPAESAA"
BASE COUNT         1919 a         3708 c         3642 g         1948 t
ORIGIN      
        1 atccgacgcc cgctcacgcg acacgctcac ttgcacatca acggagagtt cgtccacccc
       61 aaccggcctc ctcatcgccc ctatgttcgc gcatgcgtgg ggaagtgtta caaggggtgc
      121 tgagtgctga gtaaggcgaa cgtcagttgt tggacaatgt caagcgactg agccgatttt
      181 cacaactcag cactttcaac gaatgaagac caggagtacc aagccatgat cgccgtcatc
      241 ttcgaggtct agccggacga ggggaagacc cagcaatatc tcgacatcgc cgccgacctc
      301 cggccgctgc tcgacgacat cgacggcttc atctcaatcg agcgcttcga gagcatctac
      361 acacccggca agatcctctc gctctccttc tggcgcgacg aagcggcgct cgccagctgg
      421 cgacagctcg aagcccaccg cgccgcccag acacggggca gggccgacat cttctccaac
      481 taccgcatcc gcatcgccag cgtcgtccgc gactacggca tgaccgaacg cgaccaggcg
      541 ccggccgata gccgggtggt gcatgggtga tggttcgtga cgcgtgacgc gtgacgcgta
      601 atgcgtgacg cgtggagttg tcagagtccg cgttacgcgt cacgcattac gcgtcacgaa
      661 tccccgcgct tgcaccccac cccaccctct gcgatcctgc cgggtgacat gccatgcagg
      721 acaggacgag gggagcgaga cgatgccgac acgcaacgac cactggagcc gcgagccggg
      781 ccgctgggag acaagcctgc agccggaggc ctgggcgacg attccgacga tcgggacgcc
      841 gcgaccctcg ccggatggcc ggacgatcgc ctactcacgc ggctacgacg gccggctgga
      901 tatctggacg ctcgatctgg cgaccggcag gacgatgcag ctgaccgacg tcgcccgccc
      961 gcagggagcc gacctcagcc agcggcaggc ctcgtcgatc gcctggacgc cggatagcag
     1021 cacgatcgtc ttcgcctcga atcaggaggg caagctctgg tcggtcccgg ccggcggcgg
     1081 tccgtcgaag ccgatcgacg aggcggccgg caaccaccac tcgccggtca tctcgccgga
     1141 cggcacgcag gtcgcctacg tcgccgaacg gggcgaatcg gtcgacatct tcgtggctgc
     1201 catcgacggt cgctgggtgc gccagatcag cagccacgac aacgatgagt acgtcgccga
     1261 gccgagctgg tcgccggatg gccgctcgct gctttacatg cagtggccac attacgatat
     1321 gccctgggac gaggcggccg tcgtcgttgc cgacctggag tcaggcgagc ggcggatcgt
     1381 cgccggcggc gagcgggtcg tcaacaactg gccacgctgg agtcaggacg gccagtcgat
     1441 cgtctacgtc tccgatcgcg gcggcgagca tccgaacctg tgggaagtcc gccccgacgg
     1501 ctccgggatg gagcggctgg tcgaggcgcc caacgacctg ctctggccgg tcgtctcgcc
     1561 ggatggccgg acggtcgcct tcaccaagaa cgaggacaac gagggccacg tctgcatcat
     1621 cggtgccgat ggccatgtcc gtcagctcac caacgagcac ggcgtccaca ccgagctggc
     1681 ctggatcgac gagcgccgcc tcgtcggcgt ctatcagtcg ccgctggcgc cgcccgacct
     1741 ctgggagatc acgctcgacg gcgagcgtcg ccagctgacc ttctcggcaa ccggcggcgt
     1801 tgtcggcggc gatctggtcc tgcccgaggt catcacctgg caatcgcgcg acgggatgac
     1861 gatccacggc acgctcttca gcccgcctga gatccagccc ggcaagcacc cgctgatcgt
     1921 ccacatccac ggcgggccgg tcgggcaggc caacaagacc tggattccgt ggattcagta
     1981 cctcgtccag cgtggctacg tcgtcctcgt cccgaactat cgcggttcga agggctacgg
     2041 ccggacgttc atggaggccc tctacggcga ctggggcggc ggcgacctgg acgacaacat
     2101 cacaggagcc gaagccgtca tcgcccgtgg cgcggtcgac ccgacccgtg tcgtcgcgac
     2161 cggcggctcg gccggtggct acagcacgct gatctgcatg acccgcgcgc cggaggtctt
     2221 caaggccggc atcgcccgtt acggcatcgc caacctggcg accttcacgg acaagacctg
     2281 gatcttcgag cgccactacc tcgccaagct gatgggtggc accggcggca gccgttccga
     2341 cctctaccgc gagcgctcgc cgctgacaca tgtggacaac gtccgcggcc cgctgctgat
     2401 cctgcagggc gaggacgaca tcgtctgcca ccgctcggag atggacacga tggcggccgc
     2461 gctggaggca gccggcaagc acgtcgaata caccgtctac cccgacgaag gccacggctg
     2521 gaagcacgtc tcgaccatca tcgacgacgc ccgccgcagc gacgacttcc tggtgcggat
     2581 ggtgctgaat cggtaacggg gagtatggga tgtggggatg gttaacgaag cacgtatccc
     2641 acctgggcag cgttgctgag gattgcttcc cacccccgcg tcccacgtcc cacgtttcta
     2701 tcctcatttc ttagtgtttg tttagtaggt atccgtagta ggtgtggatt gtgtggataa
     2761 ctgccttgct gcctgtctgg acggggaaaa tgggcttgat gaaccggctg atgagttatc
     2821 cccatctccc cacatgcgaa ctgtgacaga ttcggcaccg gtgagttatc agtttgctgt
     2881 cgggacttgt ccacggccga gaacgacggt tatcgacgtt ccacaggtgt acgtacacat
     2941 aggtatcccc atttccccac agttgtccac atcccaccgc cgagttatcc acaacggggg
     3001 ataaccgatc gcgacagcag tgacgcgttg ccagatagtc acagtcaagc atcaggtggt
     3061 gcggtgacgt catggcctgg atggagtgtc aatggtgtcg ttgcggggat cagtggacgg
     3121 tagcgaatgc attccaatcg aaccattcgc tcaggaatgg ttgcgcgagc gatcgtggcc
     3181 catcgacctg gatgagccct gcgccttggg cagtcgtcaa cgtcaattgt cccagccaga
     3241 cgcgatacag cgcgctgata tctgtgttga ccaacaggtc gatctcaaac cccggatgat
     3301 gcaggcagat cgtcacgtca gtagattcca gcaacagcca gtagctgcct gtccagccgc
     3361 cggagaagtc aaactggatg acgatgcggc gctcggggag tgcgttggtg ttcacgcgcc
     3421 ggcgcatcca ccagagcagc agccagggat cctgctcctc cggtcgcggt tcaccgaacg
     3481 cccagcgaaa tccccaggcc gccattccat caatgacgag gcgcaagtcc tgtccagccg
     3541 gtgtcagttg gtaggttgtc aggcgaccgg cgccacctgg atagcgttcg ataatgccgt
     3601 cgtcttccag ccgttgcagg cgatcgacca gaaccgagcg cgagatgccc ggcaagctgc
     3661 ggtggagcgc gttgaatccg tcgattccag cgagcaactc gcggatgatc agcggcgtcc
     3721 atcggtcggc aatgatctcc gaggtgcgcg ccactgggca gtattggccg taggtgcgtt
     3781 tcgccacgcc tcatcctcgg ttcccggcaa gagcgcgatt cgtctcgtgt ccttcatgac
     3841 ccactataca gccaactggc tggatcacta gtctgaaagt cgcactaggc agccttcgtg
     3901 atgctggcca aactgaactg cgtcgcagtc cattggggat cgccggcatc gaagggacgt
     3961 tccatgcagg ctcaggcaac gacgacagac agcgggatag accggtcgat tcttgatgaa
     4021 ctgattgccg cggtccgctg cccggttctc cttccgggca gcagcgacta cgatcaggca
     4081 cgagcactcc acaacggact catcgaacga cgcccagccg tgatcgtgca gtgtggtggg
     4141 gtcgctgatg tggtcgcctg tgtcaacgtc gctcgtgagc gcgggttgct gctgtccgtc
     4201 aaaggcggag ggcacaacgt cgccgggaat gccgtcaatg acggcggcat cgtcatcgac
     4261 cttgcacgga tgacatccgt ccgtgtcgat cctctggctc gcacggcgcg ggtggagggc
     4321 ggggccaggt gggcagacgt cgatcgagag acgcaagtct tcggtctggc cgttcccggc
     4381 ggcgtcgtct caacgaccgg tgttgccgga ctgacactgc acggtggcat gggctatctg
     4441 cgtcgcaagc atggcctgag catcgacaac ctcctctcgg ttgacatcgt gatggcggat
     4501 gggcaggtcc gctcggtaag cgcggccaac gaccccgatc tgttctgggc ggtgcgcggt
     4561 gccggcagca acttcggggt cgtcacgtca tttgagttcc agctccatcc cgtcggcccg
     4621 ctggtgacgc tctgcgctgt cttctacgcg caggctgatg ccgagcgagt tgtgcgcgcc
     4681 tggcgagatg tcgtcaccat ggcgccggat gagctcagct ccttcgctgt cttctggagc
     4741 gtgccggccg agccaccact ctttccgtcg gagttgcacg gcacgcccgt cgtcatcgtc
     4801 aatgcggtct acgcgggggg cgctgaggag ggcgaacgcg cgatccagcc acttcgcgaa
     4861 ctggccacac cgctcatcga tctgagtggc ccgctcccat accaggaggt ccagaccctg
     4921 ttcgatccgt tcttcccgcc gggactgctc tactactgga agtcgctcaa tctggaccac
     4981 cttgacgacg ctgtgattga cgaggtctgc cggctggctg ctgagcgtcc gtcggagcga
     5041 tcggatctca tgctctggtg tcagggaggg gccataggcc gtgtcgcagc cgaggcgact
     5101 gcgttcggcc ggcgcgatgc gccgttcatg ctgctcttcg attccacgtg gcggaatccg
     5161 gaagactcga cccgcaatat cgcctggaca cgctcggcct ggtcatcgat gcagcagtat
     5221 tcgactggcg ggggactgta tctgaacttc cccggcctcg gtgaggagag tgccaagctc
     5281 ctgcgctccg ctcatggagc caactatgcg cggctacgcg cgttgaaggc gacctacgat
     5341 ccgaccaatc tcttccgaag caatctcaac attccaacga ccggctgaat gtacgtcgct
     5401 ggaagggaac gactcgcgag cagtttctcg ccggctcgat gaggctggac gttcaggttg
     5461 tgatcgtcgc ggtgtcggtg tatcgtgcgc gtatcgatga cacgttcggc caacgcaagg
     5521 tcgcaatcca gcatgcactc atcgctgccg gccgatccgc cccgaccgat ccattcgccg
     5581 acgcccatcg cgccgggacg cgttgttgcg ccggtccagt cgttgccggt cccgctgacc
     5641 ccactggtcg gacgcggacg ggaagtggag ttggcggccg ggctactgca gacggccaac
     5701 gttcggttgc tgacgctgag cgggccgggt ggcgtcggca agacccggct gctgttgcgg
     5761 cttgccgagg aactggcagg cgtctttccg ggcggcctca ccttcgtgcc gctggctcca
     5821 atcgatgatc ctcgcttgct cctgccgacg attgccggag cactcggcct gcgcgatgac
     5881 ggtggcaatg atctcaccgc gcaactggcg cacgcgttga acggctggcc tcggctcctg
     5941 ctgctcgaca acttcgagca gatcgtcgaa gccgggccat cgctgaccga gctactggcg
     6001 gtctgtccgg cgctcaagat cattgtcacc agccgcgtcg tcctgcgggt gacgggagag
     6061 caagaactgg tcatcccgcc gctgcacgtg ccgggcgcct cgcgctcccg ctcactggac
     6121 gagatcgcca ccagtgaggc catcgcgctc ttcctgttgc gcgcgacatc cgtcaatccc
     6181 tcgttcacgt tgaccgagga gaacgccccg gccattgtcg aggtctgccg gaggctggat
     6241 ggcctgccgc tggcgatcga gctggcggcc gcccggacga aggtgctctc gccgcaggcg
     6301 ctggccgagc ggctcggcaa tcgcctccag gtgctgatcg gcggtccgcg tgaccagccg
     6361 gcgcgcctcc agacgatgcg cggcgcgatc gcctggagct acgacctgct ggagccgaac
     6421 gagcaggagc tgtttcgccg gctctccgtg tttccagccg gtttcgacct ggatgccgcc
     6481 gagcaggttg cggggtcgga cctcgtgctg gacggcatca cctcgctggt cgacaagagc
     6541 ctgctgcaac agaccaacgg gcatggcgcc gagccgcgct tcacgatgct ggagacgatt
     6601 cgcgccttcg cgtccgagca gcttggcgcc agtggggagg aacacgagac gcgacagcgg
     6661 cacgccggct ggtgtcaggc gctcgtcgcc gccggggcag cgacgatgcg cgcgacgccg
     6721 gatcagtccg cgctcaaccg gctcgaacag gagcacgaca gcctgcgggt cgccctgggc
     6781 tggctgctcg acaccggcgc cgtcgaggac gccgcccgga tggtcgtcgg cgcctggtgg
     6841 ttctggttca cgcgcagcca tctgcgggcc ggccgggaat ggtcagtccg tgcgttcgcc
     6901 cggctcgacc gtccaccgga cctgttgtgg ctgcggctga acagcgtgac gggctggttc
     6961 gcggaagctg tcggcgagtt cgagacggca ttagcgatgc atcagcgcgg tttggaggtc
     7021 gcacgcgagc tcggcgatcc ggagtcgctc ggggtcgccc tctacgcgct ggcggatgtc
     7081 gtcgacgagc agcgcgagac ggagcaggcg ctcacgttgt tccgcgaggc cgaagcgatc
     7141 tttcgggagc tggacgccgc gccgtggctg gccgtcacgc tgaactcgat cggggcgatc
     7201 tatcgcgaga tcggtgagct cgatctggca atcgtcctga tcgaggaggg gctggcactg
     7261 gcgcgcgagc gcaaccacac ctggatcatc gcgctctccc tcggacacct cggccgggtc
     7321 taccggatgc ggggcgacct ctcgcgcgcg atccagctgg accggcagac ggtccgcctc
     7381 tggcatgagg tgggcgactg gtggcggatg tcgcgcgcca tcaacgagct gggtgtcgcc
     7441 gcccagctca ccggtcatca cgactacgcc gcgcggctgc tgggcgggtc cgaagcgatg
     7501 cgcgagcaat acggcgccgc cttcatgctg ttgctgacga gcgcttacga acaggcgctg
     7561 accgagacgc agcgccatct cggcgacgag gggttcacgg ccgcctggga cgcgggtcgc
     7621 tcactgagtc tggaagagtt gctcgatctg gcgaccaaca cgccggtcac gcaggttgaa
     7681 gccaggccac gcgagacttc caccgccggc ctctcggccc gcgagatgga tgtcctgcgg
     7741 ctgctcgtcg ccggctactc cgaccgccag atcgccgagg atctcttcat cagccaccgc
     7801 actgcccagg gccacgttgg cagtatcttc aacaagctcg gcgtgaactc ccggacggct
     7861 gccgcgacga ccgccatccg gctcgggctt gtcggaacgc ctgagcagca ggatgccggc
     7921 gggaatacgc tgctctgaca gcgcgaaccg ctccgcgtca ggccgctcgc cgacgcggac
     7981 ggggcgtgga cgcaccctcc caggttgcga aacggttgac tttcatgcca ttgccgatca
     8041 agatgcagtc gaacatcatc cagcactcgc acgcgattga gtactgccat ggcgacctcg
     8101 atgacgccac tccttaccac ggcaggttcc tccagcggac cgcgtctgcc aacatcgctc
     8161 accccgttga ttggccgcga gcaggagatc gccgcgctgc gcgcgttcgt gctcggtgac
     8221 ggaacccgcc tgctgacgtt gaccggtccg ggtggcagcg gcaaaacccg actcgcccag
     8281 cagctcgcga ttgacctcgg taacgacttc gccgccgggt cacgcttcat ctcgctggcc
     8341 tcggtgactg agccggaaca cctgcttcag gcgatcgcgg ttgagatggg catccgtgag
     8401 gaagccaatc gatcgctggc cgacctcgtc ccgatgaccc ttcgccagca cgagctgctg
     8461 atcgtgctgg acaacttcga gcaactggtc gaggccgcgc ctgtcgtcag cctgctgctc
     8521 ggccagtgtc tgcgactcgc catcatcgtc accagtcgcg tgccgcttca cgtgcagggc
     8581 gaacaggagt atccggtgcc gccgctggcg ctgcctggcg cacaacgagc ggcgccggtc
     8641 gaagagctgg caagctgcgc ggcgattgca ctcttcatcg cgcgcgcccg ctcggtcgat
     8701 cccggattcg tactggacga acgcaacgcg caaacggttg ccgaaatctg cctccggctg
     8761 gacgggttgc cgctcgcgat cgagctggcg gcggcgcgga tgaagatcct ctcggccgag
     8821 gcgttgctgg cgcggctcgc caaccggctg cgcatcctga tcggcggcgc gcgcgatctg
     8881 ccggcacgcc agcgcacgct gcgcgacacc atctcctgga gctacgatct gctctccccc
     8941 gacgagcagg cactcttccg ccgcctggcc gtcttccacg gcggcttcac gctggatgga
     9001 gccgccgctg tcgccggcga tatcgacgcc ctcgacggtg tcggctcgct ggtcgacaag
     9061 agtctgttgc gccgcgccga cgctgccggg atggcgcgct tcacgatgct ggagacgatc
     9121 cgtgagttcg cactcgaact gctgatgacg agcgacgaag ccgccgacgt tcagcgctcc
     9181 catgcgacct ggtacgcgga actggccgag gatgcgcgaa ccggtatcta cagcagtgag
     9241 cagcgtgtct ggctcgactg cctggaagtc gagcatgaca atctgcgcgc cgcgatgacc
     9301 ttcgccgccg aacacaacga caatgcgttg ctgttgcgct tcacggccgt cctcggggat
     9361 ttctggacga tgcgcggcca cttcagcgaa gggcacggct ggctcaagcg ggcggtcatg
     9421 gccggcagcg cgaatccgtc gccggcgctg gcacaaacgc tctccggcgc cgcgatcatg
     9481 tcaaccgtca tcggagaacc ggacgcggca cggcaattcg ccgaagacgg cctccgcgtc
     9541 gcgcgcctga cgggcgacgg gttcagcatc ggtatcgcgc tcttctgttg cgctgacgtc
     9601 gccgaggagg tcggcgacca gaacgttgcc gtcgatacct acctgcaggc ggccgaagtc
     9661 ttccgttcgc tggaggagtg ggtgctgctc gcgttgacgc tggcgaatgt cggcgccacc
     9721 tgtgtcagcc tgcatcgcta cgacgaggca atcccgccac ttcaggaagc gctggagctg
     9781 tcgacgaccc atctgttcga cctgggcatt gggatgtgct cggccaacct gtcgcggatc
     9841 gaatgggcgc gtggcgatct ggtcgccgct cgcgatcatt cgcgacagag cctccaggtc
     9901 tggttggtac tgggcgacca gatgcggatc gtccggctgc tggagcggat ggcagcgatc
     9961 gccgccgccg accgtcagcc cgttctggcg acgcggctcc tgagcgcggc cgaggcccgt
    10021 ttcgacagtt tcggcgtggc gcgcacggcc gggtcggttc aggcgatcga ccaggcgatc
    10081 aagacgatcg aaccgcaact gagccgtgag gccttcgccg gtgcctggga tgccggcagc
    10141 gcgctgtcca tcgacgaggc cgtcgcggag gcattggcgt tcgagctgat gaccgcgcca
    10201 accgccccaa cgccggcaac tgaccgctcg cttctgagcg cccgcgagca ggaggtggtc
    10261 cagctgctgg tggccggcta ctcagaccgg cagatcgcgg aatcgctgtt catcagccac
    10321 cgcacggcac agggccacgt cgccagcatc ttcaacaagc tcggcgtgaa cagccggacg
    10381 gctgccgcga cgaccgccat ccggctcggg atcgtcagcg acggtggccc gccggcatag
    10441 gctgagcgca atatcatcga aaacacgtac tccgatctac gcaatttgcc cgatgcgcat
    10501 cggccgagcg tgcctcacaa tacgtatatt ccggcaacgg cgccgggaac gcggctccaa
    10561 cgaagggtca gaccgatgca ggcaacgatg caggcaacga ccgacacgac cggcctggtg
    10621 gcacagggat ttacagcgga gcagatccag cgacttgagg agctccgcgc cgtctacccg
    10681 atgatcgaga tggtcgagaa catcgagcag ttgcggcgga tcgtcttcct caagtggctg
    10741 catgagcacc gggacgaccc ggccgacagc attgatacgg cggcctgaac cagccaacga
    10801 cggcacgtgc gggcgcccct gaaccgatca ttccggcaag cgatcgagag cgaagggacg
    10861 agccgagatg atgatccaga ctgtcaccca cccggcatgc gcttcggcga cctgcccgcc
    10921 cgtcgatcac atctcgaccc cgcataccgt cgtcgcggcg gaagggtccg aactggctgc
    10981 ccggctgcgc tcaaacggct ggatcgcccg cgccggctgg cgtcgctaca ccgctggccg
    11041 cccggccgcc tggctcgtcc gcttcgagtg cccgtcagct tgccctgcgg aatccgccgc
    11101 ctgatcgttc cccaatcaat cgatcattcc ggcaggggcg atatcgcaat atcgcccgtt
    11161 ctcgtgcccg gcgctttgga acggtgcgac attgcgatac cggctgtaac ggattga
//
LOCUS       DASUGJ010000399         3511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2007459, whole genome shotgun sequence.
ACCESSION   DASUGJ010000399 DASUGJ010000000
VERSION     DASUGJ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007459"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1150
                     /gene="selD"
                     /locus_tag="VFV93_08755"
     CDS             101..1150
                     /gene="selD"
                     /locus_tag="VFV93_08755"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:152..154,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX5165470.1"
                     /translation="MQSRDEIRLTTLASCAGUAAKLAPGALAQVLAPLAQRSDPRLLV
                     GLQTSDDAAVYQLADDLALVQTVDFFPPVVDDPYLYGAIAAANSLSDVFAMGGEPMFA
                     LNIAAWPDNLPLEYLSEIFRGGADKAHEAGIVIAGGHTVTDDEPKYGLVVTGRIDPSV
                     VLTKAGAQPGDRLFVTKRFGTGLVTTAIKQEAATEEDADAAVQSMLQLNAAASRLARE
                     VGVNACTDITGFGLLGHLTEVAAKSGVAAEIDHAAIPWLPGVDRYAAEGRFPGGANRN
                     RDFYETLPEVGVTFADGVSAEIQRLLFNPETSGGLLLSVAAERGDALLAAFDAAGHGI
                     WELGTVRAGRGIVVR"
     gene            1131..1985
                     /gene="rsmA"
                     /locus_tag="VFV93_08760"
     CDS             1131..1985
                     /gene="rsmA"
                     /locus_tag="VFV93_08760"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX5165471.1"
                     /translation="MASSSAEREPGWRARLRELGLTPSKALGQNFLHDQGIVRRIAES
                     AEIGPDETVLEVGPGLGILTEELAARAKRVVAVELDDRLAAYLPTVLPPNVEVVHADA
                     LEIDPAALAGPDYRVVANLPYSIGNAILRRMLEALPPPRTLTVMVQREVAERIAASPP
                     EMSLLAVGVQFYGTPKILFRIGGGAFIPPPRVESAVLHITTQPPLLPASEHPAFFRVV
                     QSGFAQRRKQLGNTLSTGLFIERVAVVDGLTRAGIAPTERAERLTVADWVRVYHAFRN
                     AGWLRDDQ"
     gene            2151..3038
                     /locus_tag="VFV93_08765"
     CDS             2151..3038
                     /locus_tag="VFV93_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165472.1"
                     /translation="MRRLIAVTPAKVNLGLEIIRKRADGFHELVSVMQAVSLFDTFVW
                     SETGGPFDYSGPEYVERDRDLAWRALATASDRERWTGRLQVVKRIPLAAGLGGGSSDA
                     ALALRLAFPDADDDELAQRAAAYGSDVPFFIRGGTALATGTGTELTPLATPDLWLVIA
                     TPPVDIPEKTQALYSGLEPEDFSDGANVRDIIARLKRGEQLPERLPNAFTRQLLGHSV
                     IRYAYDRLRGAGASRVSASGAGPTLYAVTASHREAARIAAELHEAGPSPGVVQVVRTV
                     ASRGERASIRAMAAALRGS"
     gene            3071..>3511
                     /locus_tag="VFV93_08770"
     CDS             3071..>3511
                     /locus_tag="VFV93_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5165473.1"
                     /translation="MQETVLNGRYRLGEVLGEGGMAVVYRAQDVLLNRPVAVKVLRSQ
                     YAADASFLRRFEREAQSAAGFSHPNIVNVYDVGTDGDQHYIVMEYIRGPSLKELIRRQ
                     GPFSVDGAIFIIGQVTSALDYAHHRGLIHRDIKPQNILVDRDGNA"
BASE COUNT          566 a         1162 c         1188 g          595 t
ORIGIN      
        1 cgaggcgatt ggtgctaacc tcgttcggag tatccttcca gagcgatgtg atgcaccacg
       61 cgtcacgcat cacgcgtcac gcgtcacgac cggaggcccc atgcaatcgc gcgacgaaat
      121 acggttgacc acgctggcgt cctgcgccgg ctgagcggcc aagctcgcgc cgggggccct
      181 ggcgcaggta ctggcaccgc tcgcccaacg cagcgacccg cgcctgctgg tcggcttgca
      241 gaccagtgat gacgccgctg tctatcagct ggcggacgat ctggcgctgg ttcagacggt
      301 ggacttcttc ccgccggtcg tcgatgaccc gtacctgtac ggggcgatcg cagctgccaa
      361 ctcgctctcg gatgtcttcg cgatgggcgg cgagccgatg ttcgcgctca acatcgccgc
      421 ctggccggac aacctgccgc tggagtatct ctcggagatc ttccggggcg gcgccgacaa
      481 ggcccacgag gccgggatcg tcatcgccgg tgggcacacc gtcaccgacg acgagccgaa
      541 gtacgggctg gtcgtcaccg gacggatcga tccctccgtc gtgctgacca aggccggcgc
      601 gcagccgggt gaccgcctgt tcgtcaccaa gcgcttcggg accggactcg tcacgaccgc
      661 catcaagcag gaggcggcga cggaggagga cgctgacgcc gccgtccaga gcatgttgca
      721 gctgaacgcg gctgcctcac ggctggcgcg tgaggtcggt gtcaacgcct gcaccgacat
      781 caccggcttt ggcctgctcg gccatctgac cgaggtggcc gccaagagcg gcgtcgcggc
      841 cgagatcgac cacgccgcga tcccgtggct gccgggagtc gaccgatatg ccgctgaggg
      901 acgcttcccc ggcggtgcca accgcaaccg cgacttctat gaaacgctcc ccgaagttgg
      961 cgtgaccttt gccgatgggg tcagcgccga gatccagcgt ctgctgttca atccggagac
     1021 ctccggcggg ctgctgctgt ccgtggctgc cgagcgaggc gacgcgctgc tggctgcctt
     1081 cgacgcggcc gggcatggca tctgggagct gggaacggtc cgcgcaggtc gtggcatcgt
     1141 cgtccgctga gcgcgagccg ggctggcggg cgcggctacg cgagttgggc ctgacgccca
     1201 gcaaggcgct cggtcagaac ttcctccacg atcaggggat cgtccgccgg atcgccgaga
     1261 gcgccgaaat cgggccagac gagaccgtcc tcgaagtcgg tcccggcctc ggcatcctga
     1321 ccgaagagct ggcggcccgc gcgaaacgtg tcgtcgccgt cgagctggac gaccggctgg
     1381 cggcctacct gccgactgtc ctgccgccga acgtcgaagt cgtccacgcc gacgcgcttg
     1441 agattgatcc ggcagcgctg gccggaccgg actaccgtgt cgtcgccaac ctgccctatt
     1501 cgatcggcaa tgcgatcctg cgtcggatgc tggaagccct gccgccgcca cgaacactga
     1561 ccgtcatggt ccagcgcgaa gtcgccgagc ggatcgccgc cagcccgccg gagatgagcc
     1621 tgctggcggt tggcgtccag ttctacggca cgccgaagat cctcttccgc atcggcggtg
     1681 gcgcgttcat cccgccgccg cgcgtcgaat cggccgtcct gcacatcacg acccagccgc
     1741 cactgttgcc ggccagcgag catcccgcct tcttccgcgt cgttcagtcc ggcttcgccc
     1801 agcgccgcaa gcagctcggc aacacgctct cgaccgggct gttcatcgag cgcgtcgccg
     1861 tcgttgatgg gctgacacgg gccggcatcg cgccgaccga gcgtgcagaa cggctgacgg
     1921 tcgccgactg ggtgcgggtc tatcatgcgt ttcgaaatgc cggttggttg cgggatgatc
     1981 aatgagcatc gtgccgccgt aggggcgaat cgatgattcg cccaatcaat tcggccgcgt
     2041 tcagcggcca caatccgcca atcgccattg ggatcggctg gacgccgatc agattgtccg
     2101 cgcgaatcac cgattcgctc ctacggcgga acaattgatt gaggttgtcg atgcgtcgac
     2161 tgattgccgt cacgcctgcc aaggtcaatc tcggtttgga aatcatccga aagcgcgcgg
     2221 atggctttca cgagctggtg tcggtcatgc aggccgtcag cctcttcgac accttcgtct
     2281 ggagcgagac aggtgggccg ttcgactaca gtgggccgga gtacgtcgaa agggatcgcg
     2341 atctggcctg gcgggcgctc gcgacggcgt cggatcgtga gcggtggacg ggtcggttgc
     2401 aggtcgtcaa gcgcatcccg cttgcggccg ggctgggcgg cggttcgtca gatgccgcgc
     2461 tggcgttgcg gctggcgttt ccagacgcgg acgatgacga actggcacaa cgagccgccg
     2521 catatggctc ggacgtgccg tttttcatcc gtggtgggac ggcgctggcg actggtaccg
     2581 ggaccgaact gacgccactg gcgacgccgg atctttggct tgtcatcgca acgccgccgg
     2641 tcgatatccc cgaaaagacg caggcgttgt atagtggact tgagccggaa gacttcagcg
     2701 atggcgcgaa cgtacgcgac ataatagcga gattgaagcg tggcgagcag ttgccggaac
     2761 gcctgccgaa cgcgttcacc cgacagctcc tcggccactc cgtcatacga tacgcttacg
     2821 accggctccg gggggcaggt gcgtcgcgcg tctcggccag tggtgctggc ccgacgctct
     2881 acgctgtgac tgccagccat cgcgaagcgg cgcggatcgc cgccgagcta catgaagccg
     2941 gcccgtcgcc gggggtggtc caggtcgtcc gaacggtcgc cagtcggggc gagcgggcgt
     3001 cgatccgggc aatggcggcg gcgctgcggg gcagctagcg ccggcagctg acgggcagag
     3061 aggacctgtg gtgcaggaga cggtcctgaa tgggcggtat cgccttgggg aggtgctcgg
     3121 ggagggcggc atggccgtcg tctatcgcgc ccaggatgtc ctgctgaacc ggccggtcgc
     3181 cgtcaaggtg ctgcgttcgc agtatgccgc cgacgcgagc ttcctgcgcc gcttcgaacg
     3241 cgaggcgcaa tcggccgccg gcttctcgca tccgaacatc gtcaacgttt acgacgtcgg
     3301 caccgatggc gaccagcact acatcgtcat ggaatacatc cgcgggccga gcctgaagga
     3361 gctcatccgc cgccaggggc cgttctcggt cgatggtgcg atcttcatca ttggccaggt
     3421 cacctcggcg ctcgactacg cccaccatcg tgggctgatc caccgcgaca tcaagccgca
     3481 gaacatcctc gtcgatcgcg acggcaacgc c
//
LOCUS       DASUGJ010000400         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2007658, whole genome shotgun sequence.
ACCESSION   DASUGJ010000400 DASUGJ010000000
VERSION     DASUGJ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007658"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..501
                     /locus_tag="VFV93_08775"
     CDS             79..501
                     /locus_tag="VFV93_08775"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5165474.1"
                     /translation="MEKSPRTVDDLNAWSPGTLPGLLGIRITELIEGGVTGEMPVRPD
                     LLAPNCYLHAASVVGLADTVSGYGCRANLPDGAIGFTTIELKSNFLGTAVEGVLDCEA
                     TLQHGGRTTQIWDAIVTDRTSGRKIALFRCTQLVLYRR"
     gene            507..1091
                     /locus_tag="VFV93_08780"
     CDS             507..1091
                     /locus_tag="VFV93_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HEX5165475.1"
                     /translation="MGETTALAPNDPIYFRHARTIGAVAIVAYVLTVVAANGAIDRFG
                     TVSVGFGLTAPAGVYFAGLAFTLRDIAHDTLGRWYVLGGIAAGSIISLVQSDNATIPG
                     GVTSIAVASAAAFLLSETFDFAVYTPLRERNWIGAVMASNVVGLVADSVVFLWLAFGS
                     LDFLGGQIVGKGWMTVAAVIVLTFWRRATRSPSS"
     gene            complement(1206..2090)
                     /locus_tag="VFV93_08785"
     CDS             complement(1206..2090)
                     /locus_tag="VFV93_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5165476.1"
                     /translation="MAGPTYGKNPPANQLDFGIVTGQHWRSWDELVEQWQWADESGLD
                     SAWAFDHFFSLRDGELGPCLEGWTLLAALAVKTSNVQIGLMVNGNTHRNPAILAKQAV
                     TVDIISGGRSILGVGAAWHEREHQAYGIDFPSPRERVDRFGEAMELHRRLETQERATF
                     EGQYYRLDNAPFEPRPVFGHIPILVGSTGKRMLHHVARYADQWDGGGTPDELRATGER
                     LKAACAEIDRDPSEIRWAYSTGSDKFDSVDTFRKHVNDFAAVGVRSFLFTLPGGSPNA
                     TIRTIANEVIPELREEWK"
     gene            complement(2179..>2535)
                     /locus_tag="VFV93_08790"
     CDS             complement(2179..>2535)
                     /locus_tag="VFV93_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165477.1"
                     /translation="AVLWAHSFFWLLLTPTIGVSAAIVAFLSFTAGELLFMPITALIP
                     VRLAPVHLRGRYFSLSSVVWGGSYALASLVGGVALDMSNPALLWPVMIGLMLVGGAGA
                     LRLRTSVRLEPVPLSS"
BASE COUNT          405 a          825 c          872 g          433 t
ORIGIN      
        1 cgctcggctt ctacaatacc gaagaggatg tcgaccgctt catcgccggc gtgaccgcca
       61 tccagggttc gctctaggat ggagaagtcg ccgcggacgg ttgacgacct caacgcctgg
      121 tcgcccggca cgttgccggg gctgctgggc atccgcatca ctgagcttat cgaagggggc
      181 gtgaccggcg agatgccggt ccgccccgac ctgctggcac cgaactgcta cctgcacgca
      241 gcgtcggtgg tcggactggc cgataccgtc tccggctacg gctgccgcgc gaacttgccg
      301 gacggggcga tcggcttcac aacgatcgag ctcaaatcca acttcctcgg gacggccgtc
      361 gagggcgtgc tcgactgcga agcgacactc cagcacggcg gcaggacgac gcagatctgg
      421 gacgcgattg tgaccgaccg gacaagcggc cggaagatcg cgctcttccg ctgcacgcaa
      481 ctggttctct accgacggtg atgagcgtgg gcgagacgac cgcgctggcg ccgaacgatc
      541 cgatctactt ccggcatgcc cggacgattg gcgcagtcgc gatcgtcgcg tatgtcctga
      601 cggtcgtggc cgccaacggg gcgatcgatc ggttcggcac cgtctcggtc gggttcgggc
      661 tgactgcgcc cgccggtgtc tacttcgccg gtctggcgtt caccctgcgc gatatcgccc
      721 acgacacgct cggccgctgg tacgtccttg gcgggatcgc ggccggcagc atcatctcgc
      781 tcgttcagtc cgacaacgcc acgatcccgg gcggggtcac ctcgatcgcc gtcgccagcg
      841 cggcggcctt cctgctgtcg gaaacattcg acttcgccgt ctacacgccg ctgcgcgagc
      901 gcaactggat tggcgccgtc atggcctcga acgtcgtcgg gctggtggcc gactcggtcg
      961 ttttcctctg gctggcgttc ggctcgctcg acttcctcgg cggtcagatc gtgggcaagg
     1021 gctggatgac ggtggcggcc gtcatcgtcc tgacgttctg gcgccgggcg acgcgttccc
     1081 cgtcgtcctg agcctcccct ttgtcatcct gaggcggcag ccgaaggatc tctgctcggc
     1141 agagtgatcg gccaacgcag gagagatccc tcggctgccg cctcaggatg acaaagaggt
     1201 gcagcttatt tccactcctc gcgcagctcc ggaatgacct cgttggcgat cgttcggatc
     1261 gtagcgttcg gggagccgcc cggcagcgtg aacaagaatg agcggacgcc gacggcggcg
     1321 aagtcgttga cgtgcttgcg aaaggtgtcg accgagtcga acttgtcgct gccggtggag
     1381 taggcccagc ggatctctga cgggtcgcgg tcgatctcgg cgcaggcggc cttgaggcgc
     1441 tcgccggtcg cacgcagctc gtcgggagtc ccgccgccgt cccactgatc ggcgtagcgg
     1501 gcgacgtggt gcagcatgcg tttgccggtc gaaccgacca ggatcgggat gtgaccgaag
     1561 accggcctcg gctcgaacgg agcgttgtcg agccggtagt actgcccctc gaacgtggcg
     1621 cgctcctgtg tctcgagccg gcgatgcagt tccatcgcct cgccgaagcg gtcgacgcgc
     1681 tcgcgcgggg atgggaagtc gatgccgtag gcctggtgct ctcgctcgtg ccaggccgcg
     1741 ccgacgccga ggatcgaccg gccaccggag atgatgtcga ctgtgacggc ctgcttggcg
     1801 aggatggccg ggttgcggtg cgtgttgcca ttgaccatca ggccgatctg gacgttgctg
     1861 gtcttgacgg ccagcgccgc cagcagcgtc cagccttcga gacacgggcc tagctcgccg
     1921 tcgcgcaatg agaagaagtg gtcgaacgcc caggcggagt ccagcccgct ctcatcggcc
     1981 cactgccact gctcgacgag ctcgtcccac gagcgccagt gctggccggt gacgatcccg
     2041 aagtcgagct ggtttgccgg tgggttcttg ccgtaagttg gtcctgccat cgatcgtttg
     2101 tgctcctctg tccgacgaat ccggtcgcgg cggtcgcgtg ggacggtatc acgtcggacg
     2161 gtgaggcgtc gattcggttc aggacgacag ggggactggc tccagccgaa ccgaggttcg
     2221 cagccgcaat gcgccggcgc cgccgaccag catcagtccg atcatgaccg gccagagcag
     2281 ggccgggttg gacatgtcga gcgcgacgcc gccaaccagc gaggccagcg cgtacgagcc
     2341 gccccagacg acgctggaga gcgagaagta gcgcccgcgc agatggaccg gcgccagtcg
     2401 gaccgggatc agcgccgtga tcggcatgaa cagcagctca ccggccgtga acgagaggaa
     2461 cgcgacgatg gcggccgaga ccccgatcgt cggcgtcagc agcagccaga agaatgaatg
     2521 tgcccagaga acggc
//
LOCUS       DASUGJ010000401         9764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_479988, whole genome shotgun sequence.
ACCESSION   DASUGJ010000401 DASUGJ010000000
VERSION     DASUGJ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9764
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479988"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VFV93_08795"
     CDS             complement(<1..1139)
                     /locus_tag="VFV93_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5165478.1"
                     /translation="MALNGRNEHRTVGDAPRWAVVGTRMVTDAGLVIVGFWLAYWLRY
                     TMHVGGEVSPSSQQPFSFFYGKIALLAILVVAVFQFRGFYRMPRWATLLDEVSLIANT
                     ITTSMALVILYSFLQRFYPSRLVFILAWLAIIVLLIGKRLLVRVVRERLWLRGIGVDR
                     VLIVGAGRAGQRMMQWLFGQPQLGYQVVGFIDDDLTHESLAIATQRRVERPTYLGTSA
                     GIREVVRRHGVNEVIIALPPTAHEQMMWIVDQCRAAEIEFKLVPDLFELALDSVNIHE
                     VAGLPLIGLKPARIAGWNLVIKRTMDIALSMTVLTFGSLLMIPIVIAIKLDSEGPILF
                     RQERVGRNGRRFVCYKFRTMVRDAESMRDELLKMSNVDPRLIKNP"
     gene            1378..2295
                     /locus_tag="VFV93_08800"
     CDS             1378..2295
                     /locus_tag="VFV93_08800"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX5165479.1"
                     /translation="MERLWTPWRMTYVGGTHQEGCVFCNALAAGDDVGRLVLYRGQHA
                     FVILNLYPYNSGHAMVVPNQHVAELDALDSATRSELFELATLLTEAAKTVLHCEGFNL
                     GLNIGSIAGAGVAEHLHMHVVPRWSGDANFMPILANTMVMPELLPVTYARLRAELEGL
                     LAHRERQAVLQAGAVVVIPELGQVALRRGTTGDIALPKGHLESRESLAETAIREVLEE
                     TGIAATIAGWAGCTEFDYPPGGRSSQLHYAAYFVATGTPTPELDQHLKTDTILVPVAS
                     AAERITVPAVRAIVERVTPLLHGLCESLA"
     gene            2292..3314
                     /locus_tag="VFV93_08805"
     CDS             2292..3314
                     /locus_tag="VFV93_08805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5165480.1"
                     /translation="MNRRPTLPLIVTIVLVLPLLIGLLSPLSSAAQTPGAYIVLGDSI
                     AAGIGSSLPRSRGNAAIVTGWLEQLSGSDVPLDNLAVPGETATSFIDGGQLQRFRDSV
                     ARTKASGVPIAAVSVSLGGNELLGLDSTGLSNRQSGLDVFRARYGEALGAIRDEIGPD
                     TPLVVTTVYDLTGGDPTIQYSDSWWIEQFNSVIRQAAESEQALVADVAGQFSGRISEL
                     THYPFDVHPSNAGHRVIAQTIWTELAFDTTPPAVTTPASVTVTRPTPTIHFSVADNVA
                     VASVRVTGDDGSFAGPFQTADNEYVILLDFGSSNVEKVALTLEISDDGGNLTRQVITV
                     QRAAGT"
     gene            3331..4518
                     /locus_tag="VFV93_08810"
     CDS             3331..4518
                     /locus_tag="VFV93_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5165481.1"
                     /translation="MSVQRGAPMRVDPPKLEGFDEEIDLQGRLGAFSISDIFQMFTYT
                     EKTGTLAMIQGWNTRTITFERGRISYVAAGSRLPTIGELLVRNGRIGRDVADQHGRNM
                     SDAEFVGALLREGQINSTDIQRCQDQLLEASIYTLFLWRNCYFTFRANQLVKEGGVAV
                     SVDSTHLIIEGTRRVDEWIEISPIVPSVFMIFRRTARQPASRPARELYDVFSRVDGRQ
                     DVVAIARATKQTQFETARKLYELVRGGYIEAIPPNREKVIELFNLAVESIYLKLIMFD
                     YARAALEFENQFNRFAIDNRLKVRMSAGKILMSDATTPISGTELVDLYRLFVGIQNNK
                     FSKMFDPVIANGLMEGLYRHTDPDMQAMMRMYEFVEIEGLLMLDLFGESSAASNRDTS
                     VAT"
     gene            complement(4515..5849)
                     /locus_tag="VFV93_08815"
     CDS             complement(4515..5849)
                     /locus_tag="VFV93_08815"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEX5165482.1"
                     /translation="MANSPVSELTLSRIWQDGLFTREMRTVEGHRASVVYGGVWTHQN
                     GPDFRDAMVEIDGRLLRGAVELHLRASDWQRHGHQHDPAYDNVILHAIVEHDLPDAVH
                     GPQGEPVATVVLTDFLQAPLAELAERVFVTSLGALGSRTCLPTLANGRPDAVRDVLRR
                     AGWRRMSAKQLRFAQELERLPAGEVLYRGLLDGMGLMHNREGMAAIGERLPLALIEAT
                     CVAGKPSAIAALLGIGGFLPLAPAHAELADVTMRVAFEVEQLFAELTRERRIVPLAPT
                     VWSLNRVRPTNHPVRRLASLGALIEHASADGLLATVLGLPLDDGRSWRIWLESVRPAI
                     GRSRADQLAVNVLAPFMAAYADATGDDQLAEQVGSLWEVLPGAADDSIAKATLKQIVG
                     EQRFRVKQALESQGLHEIGRNGCRQVRCFECPIAALAVMYEPETTASAVLPG"
     gene            5975..6985
                     /gene="gmd"
                     /locus_tag="VFV93_08820"
     CDS             5975..6985
                     /gene="gmd"
                     /locus_tag="VFV93_08820"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5165483.1"
                     /translation="MSQPQKTALITGITGQDGSYLAELLLSKGYRVVGMMRRTSTETV
                     GRIGHLLDQIELVHGDLLDQLSLISLVRDYQPDEVYNLAAQSFVPTSWQQPVLTGEFT
                     ALGVTRLLEAVRLAKPDARFYQASSSEMFGKVQEVPQNEDTPFYPRSPYGVSKVYGHW
                     ITVNYRESYDLFAVSGILFNHESPRRGLEFVTRKVTHGVARIKEGEATTLPLGNLDAQ
                     RDWGYAGDYVRAMWLMLQQDAAEDYVVSTSRTHTVRRLCEIAFEAAGLHWEDHVVVDE
                     RFFRPAEVDLLVGDAEKARTKLGWSPEVSFEQMIVDMVSADIDLIRSQPRAMWPLNVR
                     YT"
     gene            7189..7374
                     /locus_tag="VFV93_08825"
     CDS             7189..7374
                     /locus_tag="VFV93_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165484.1"
                     /translation="MTQQVSRQIALMAVFTTCILLIALILIVGFNGDTGTAMSQAAPL
                     AVILGGALTFLVADSAR"
     gene            7652..8875
                     /locus_tag="VFV93_08830"
     CDS             7652..8875
                     /locus_tag="VFV93_08830"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX5165485.1"
                     /translation="MKEKPQVSKVVLAYSGGLDTSIIIPWLKENYGCEVIAFAANVGQ
                     ADELDGLEEKALASGASKLYVEDLREEFLKQYAFPTLRAGAVYERKYLLGTSHARPII
                     ASHQVAVAKLEGADGLCHGCTGKGNDQVRFELTYQALAPEMAVIAPWREWSIESREDA
                     IDYARMHEIPIRASRENIYSRDRNLWHFSHEGGQLEDPWLEPPADLFGISVSPEEAPD
                     TPHELTISFDEGWPVALDGQQLGAVELLNHLNEVGGQHGIGRIDLVENRLVGMKSRGV
                     YEQPGATILSTAHKELEAITLDRQTMHYKDLVAQRYAELVYDGLWFTPLREALDAFVD
                     VTQKRVTGDVKIKLYKGNCIIAGRRSPYSLYSETFATFGASAVYDHADAAGFITLFGL
                     PLKITALSQAAGSED"
     gene            9015..9710
                     /locus_tag="VFV93_08835"
     CDS             9015..9710
                     /locus_tag="VFV93_08835"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX5165486.1"
                     /translation="MARKSQSQSRPARKAAGNIQSSSQPETSTPKLDPLSQRRETRSR
                     GRSAAAEARRRQKRIRNIVLAVVAVLAIGLGSVFAYQRITYVEPGTKAADLGGGHVEQ
                     GTTVSDYNTDPPTSGQHYASTAPWGIHTEPVQNELQVHNLEHGGIVIQYNDSLSADEI
                     SVLESITNQCDVKLLLAPRPDMEQRIAVTAWTYYEYFDTADRDAIQKFIDAHVDEGPE
                     RIASESDRWNDCN"
BASE COUNT         1876 a         3289 c         2901 g         1698 t
ORIGIN      
        1 ttggggttct tgatcaagcg cggatcgacg ttgctcatct tgagcaattc gtcgcgcatg
       61 ctctctgcgt cacgcaccat cgtccggaac ttgtagcaga cgaaccggcg accattgcgc
      121 ccgacccgct cctgccggaa caagatcggc ccttccgaat cgagcttgat cgcaatcacg
      181 atcgggatca tcagaagtga tccaaacgtc agtacggtca ttgacagcgc aatgtccatc
      241 gttcgcttga tgacgagatt ccacccggca atccgtgccg gcttgaggcc gattagcggc
      301 aggccggcca cttcatggat gttgacgctg tcgagcgcca gctcgaacag gtccggtacg
      361 agcttgaact cgatctcagc cgcccggcac tggtcgacga tccacatcat ctgttcgtgg
      421 gcggtaggcg gcaacgcgat gatgacctcg ttgacaccat gtctccgcac gacctcgcga
      481 atgccggcgc tcgtgccgag gtaggtcggc cgctcgactc gacgctgagt cgcgatcgcc
      541 aggctctcgt gcgtcagatc gtcgtcgatg aagccaacca cctggtagcc cagctgcggc
      601 tggccgaaca gccactgcat catccgctgg ccggcccggc cggcgccgac gatcagcacc
      661 cgatcgacgc cgataccccg cagccagagt cgttcacgca cgacgcgcac cagcagccgt
      721 ttgccgatca gcaggacgat gatcgccagc caggccagga tgaagaccag gcgcgacggg
      781 taaaagcgtt gcaggaacga gtagagaatg accaatgcca tcgaggtggt gatggtattg
      841 gcgatcagcg agacctcgtc aagcagcgtc gcccagcgcg gcatccggta gaacccgcgg
      901 aactggaaga cggcgacgac cagaatcgcg agcaacgcga tcttgccgta gaaaaacgag
      961 aagggctgct gagacgatgg cgacacttcc ccgccgacat gcatcgtgta tcgcagccag
     1021 taggcgagcc agaagccaac gatgacgagg ccggcgtcgg ttaccatccg ggtgccgaca
     1081 acggcccagc ggggtgcatc cccgaccgtg cgatgttcgt tccggccgtt cagcgccaag
     1141 ccgtcgttcg tccctccgtc tgacccgcag ccgggccaca agcaggcgta agcggcacgg
     1201 agtagtcagc ccctgcgaac cggttccgcc tgaccgccga gtatagcata ccgtctatga
     1261 ccgccagtca cggcgcatac gcgaacgtac gccggatcta ctccaaatcc cggtcccagc
     1321 cttgtgtttt gctatcatcg agccccggcc gggcaggccg gcgaaaggag cgccggagtg
     1381 gagcggctct ggacgccgtg gcggatgacc tacgtgggtg gaacacatca ggaaggttgt
     1441 gtcttctgta atgccctggc ggccggcgac gacgtcggac ggctggtgct gtaccgtggt
     1501 caacacgcct tcgtgatcct gaatctctac ccctacaact ctggccacgc aatggtcgtc
     1561 ccgaatcagc acgtcgccga gctggacgcc ctcgattcgg cgacgcgttc tgagctgttc
     1621 gagctggcca cgttgctgac cgaggcggct aagaccgtcc tgcattgcga ggggttcaat
     1681 ctcggcctca acatcggctc gatcgccggc gccggcgtgg cggagcatct gcacatgcac
     1741 gtcgtcccgc gctggtccgg cgatgccaac ttcatgccga ttctcgccaa cacgatggtc
     1801 atgccggaac tgttgcccgt cacctacgct cgcctgcggg cggaactgga agggctgttg
     1861 gcccaccgcg agcggcaggc agtcctccag gccggcgccg tcgtcgtcat ccctgaactc
     1921 ggtcaggtgg cgctcagacg aggaacgacc ggcgacatcg ccctgccgaa gggtcatctc
     1981 gaatcccgcg aatcgctcgc tgaaacggcg atccgcgagg tgctggagga gaccggcatc
     2041 gcggccacga ttgccggctg ggccggatgc actgaattcg attatccgcc gggcggtcgg
     2101 tccagccagc tgcattatgc ggcctacttc gtcgcgaccg gtacgccgac accagaactg
     2161 gatcagcacc tcaagaccga cacaatcctc gtcccggtcg caagcgctgc cgagcgaatc
     2221 accgtcccag ccgttcgagc gatcgtcgag cgcgtcacgc cgctgctaca cggcttgtgt
     2281 gagtcactag catgaaccga cgtccgacgt tgcccctcat cgtcacgatc gtgctcgttc
     2341 tgccgctgct gattgggctg ctgagcccgc tgtcgtcggc agcgcaaacc cccggcgcct
     2401 acatcgtcct cggtgactcg atcgcggccg gcatcggctc aagcctgccc cgctcgcgcg
     2461 gaaacgcagc catcgtcacc ggttggctgg aacagctcag cgggtcggac gtgccactcg
     2521 ataacctcgc ggtgccagga gagacggcca ccagcttcat cgatggcgga caactccaac
     2581 gctttcgcga cagcgtcgcg cggacgaaag cctcgggcgt acccatcgca gccgtctcgg
     2641 tctccctcgg tggcaacgag ttgcttggtc tggattcgac cggtctgagc aaccgccagt
     2701 ctggtctcga cgtattccgg gcgcgctacg gcgaagcgct cggagcaatc cgcgacgaga
     2761 tcggaccgga cacgccgctg gtcgtgacga ccgtctacga cctgaccgga ggggatccga
     2821 cgattcagta ttccgattcg tggtggatcg agcagttcaa ttccgtgatc cggcaggcgg
     2881 ctgagtccga gcaggcattg gtcgcagatg tcgccgggca gttctccggc cggatcagcg
     2941 agcttaccca ttatccattt gatgtccacc catcgaacgc aggccatcgc gtgatcgccc
     3001 agacgatctg gaccgaactg gccttcgaca cgacgccgcc ggccgtcaca acacctgcca
     3061 gcgtcaccgt gacacggcca accccaacga tccacttttc ggtcgccgac aacgttgccg
     3121 ttgcatccgt tcgggtcacc ggcgacgatg gcagcttcgc tggcccgttc cagacggcgg
     3181 acaacgaata cgtcatcctg ctggattttg gatcgagcaa cgttgaaaag gttgcattga
     3241 cgctcgaaat cagcgacgat gggggaaacc tgacacggca ggtaatcacc gtccagcgag
     3301 cagctggaac gtagaggcga gaaccggtcg atgagcgtac aacggggcgc gccaatgcgc
     3361 gtcgatccgc caaagcttga ggggttcgac gaggagatcg acctgcaagg caggctcggc
     3421 gccttctcga tctccgatat cttccagatg ttcacctaca ccgagaagac cggcacgctg
     3481 gcgatgatcc agggctggaa cacgcgcacg atcacgttcg agcgcggccg gatctcttac
     3541 gtcgccgccg gcagccggct cccgacgatc ggcgagctcc tcgtccgtaa tgggcggatc
     3601 ggccgcgacg tcgccgacca gcatggccgg aacatgtctg acgccgagtt cgtaggtgcc
     3661 ctcctgcggg aggggcagat caattcgacc gacatccagc gctgccagga ccagttgctt
     3721 gaggcgtcga tctacaccct cttcctctgg cgcaactgct acttcacctt tcgcgcaaac
     3781 caactcgtca aggagggcgg tgtcgccgtc tcggtcgaca gcacccacct gatcatcgaa
     3841 ggcacgcgcc gcgtcgatga gtggatcgag atcagcccga tcgttccatc cgtcttcatg
     3901 atcttccgcc gaaccgcccg ccagcccgcc tcacggcctg cccgcgagct gtacgatgtc
     3961 ttcagccgcg ttgatggccg acaggacgtt gtcgcgatcg cgcgggcaac caagcagacc
     4021 cagttcgaga cggcccgcaa gctctacgag ctcgtgcgcg gtggttacat cgaggcgatc
     4081 ccacccaacc gtgagaaggt catcgagctg ttcaacctgg ccgtcgagtc gatctacctg
     4141 aagctgatca tgttcgacta tgcccgcgcc gcgctggagt tcgagaacca gttcaatcgc
     4201 ttcgcgatcg acaaccggct caaggttcgc atgtcggccg gcaagatcct gatgagcgac
     4261 gcgacaacac cgatcagcgg cacggaactg gtcgacctct accggctctt cgtcgggatt
     4321 cagaacaaca agttctcgaa gatgttcgac cccgtcatcg ccaacggcct gatggagggg
     4381 ctctaccgcc acaccgaccc ggacatgcag gccatgatgc ggatgtacga gttcgtcgag
     4441 atcgagggtc tgttgatgct cgacctgttc ggcgaatcgt ccgccgcgtc gaaccgagac
     4501 acgtcggtcg cgacctaacc gggcagcacg gcactcgccg tcgtctcggg ttcgtacatg
     4561 accgccagcg ccgcaatcgg gcattcgaag cagcgcacct ggcgacagcc gttgcggccg
     4621 atctcgtgca gcccctgact ctcgagcgcc tgcttcaccc gaaagcgctg ctcaccgacg
     4681 atctgcttca acgtcgcttt ggcaatcgag tcatcagcag cgccaggtag cacctcccag
     4741 agcgatccca cctgctcggc cagttgatca tcaccggtcg cgtcggcgta ggccgccatg
     4801 aatggcgcga gaacgttgac ggccagctgg tctgcccgcg agcggccgat cgccggccgg
     4861 acggactcca gccagatccg ccacgagcgc ccgtcgtcca gcggcagccc cagcacggtc
     4921 gccagcaaac catccgctga cgcgtgctcg atcagcgctc cgagcgatgc cagccgccgg
     4981 accgggtggt tcgtcggacg cacccgattc aacgaccaga cagtcggcgc gagcggcaca
     5041 atccgtcgct cacgtgtcaa ctcagcaaac agctgctcga cctcgaaagc cacccgcatg
     5101 gttacgtccg cgagttccgc gtgggccgga gcgagcggca aaaatccacc aataccgagc
     5161 aacgccgcaa tcgcgctcgg tttcccggcg acgcaagtcg cttcgatcag cgccagcggc
     5221 aggcgctcgc cgatcgccgc catcccttcg cggttgtgca tcaaccccat gccatccaac
     5281 aatccgcgat agaggacctc tcccgcgggc aatcgctcca gctcctgagc gaaacgcagt
     5341 tgcttggcag acatccgccg ccagccggcc cgccgcagca cgtcgcggac ggcgtcggga
     5401 cgcccgttgg ccagggttgg caggcaggtt cggctgccaa gcgctccgag cgaggtgacg
     5461 aagacgcgct ctgcgagttc cgccagcggt gcctgtaaga agtcggtcaa cacaaccgtc
     5521 gcaaccggct cgccctgcgg cccgtggacg gcatctggga gatcatgctc gacgatggca
     5581 tgcaggatga cgttgtcgta ggcagggtcg tgttggtggc cgtgccgctg ccaatctgac
     5641 gcccgcaaat gcagctcgac cgccccgcgc agcagacgcc cgtcaatctc gaccatcgcg
     5701 tcgcgaaagt cgggaccgtt ctgatgcgtc cagactccgc catagacgac cgacgctctg
     5761 tggccttcga ccgtgcgcat ctcgcgcgtg aagagcccat cctgccagat cctgctcaac
     5821 gtcagttcgg agaccgggga gttcgccatg acctgccggc gcactttcgc gaatgcggtt
     5881 tggaacacta ctggccggca gtctatcatc gtctgccggt cagggagcgc gcaacgctag
     5941 accttgtatc aagcgtccga tgaagggaaa cgacgtgagc cagccgcaga agacagcact
     6001 catcaccggc atcaccggac aggacggctc gtacctggcc gaactgctgc tctcgaaggg
     6061 ctaccgcgtc gtcgggatga tgcgccgcac cagcactgaa acggtcggcc ggatcggaca
     6121 cctgctcgac cagatcgagc tggtccacgg cgacttgctc gaccaactgt ccctcatcag
     6181 cctcgttcgc gactaccagc cggacgaggt ctacaacctg gcggcccagt cgttcgtccc
     6241 gacctcctgg cagcagccgg tcctgaccgg cgagttcacg gccctcggcg tcacgcgcct
     6301 gctggaagcc gtccgtctcg ccaagccgga tgcccgcttc taccaggcgt cgtcgtccga
     6361 gatgttcggt aaggtccagg aagtgccgca gaacgaggac acgccgttct acccgcgctc
     6421 gccctacggc gtctcgaagg tctacggcca ctggatcacc gtgaactacc gcgagtcgta
     6481 cgacctgttc gccgtctccg gcatcctctt caaccacgag tcaccccgac gcggcctcga
     6541 attcgttacc cgaaaggtga cgcacggtgt cgcccgcatc aaggaaggtg aagcaacgac
     6601 gctcccgctc ggcaacctcg acgcccagcg cgactggggc tacgcgggcg actacgtgcg
     6661 cgcgatgtgg ctgatgctcc agcaggacgc cgccgaggac tacgtcgtct cgaccagtcg
     6721 gacccatacc gtccgccgcc tctgcgaaat cgccttcgaa gcagccggtc ttcattggga
     6781 agaccacgtc gtcgtcgatg aacgcttctt ccggccggcc gaggtcgacc tgctggtcgg
     6841 cgacgccgag aaggcccgca ccaagctcgg ctggtccccc gaggtcagct tcgagcagat
     6901 gatcgtcgac atggtcagcg ccgatatcga cctcatccgc agccaacccc gcgcgatgtg
     6961 gccgctcaac gttcgatata cgtaggtatt aggtgttagg tgataggtgt taggggctgg
     7021 cctgtgcagg tcaaagtgac gcgttccatt ggcacagacc agcccctaac acctatcacc
     7081 caatgcctgt agcctgtcac ctcgacggct tgacaaaccc gaacgcacgt tctactatgt
     7141 gtacgtacat ttgttcggct cgtttgttct agcgtcgagg agtcaacgat gacgcagcag
     7201 gtttcgcggc agatcgcact gatggcggtt ttcaccacct gcatcctcct gatcgcacta
     7261 atcctgatcg tcggcttcaa cggtgatacc ggcacagcca tgtcccaggc cgccccgctg
     7321 gccgtcattc tcggcggcgc ccttaccttc ctcgtcgccg attcggcgcg ctgaacccgg
     7381 tacgccttcc atcccagcgt gagtgcggta cagcttgcta tcgatcggat tatgacgact
     7441 gcttgacccg tctcgatccg ccgtggtacc ttctgaaccg tcaggtttgt gacactcttc
     7501 acgaacatgg agcgcacttg ctcgtggccg gtgatagtct ttcaactcag cagaggtgca
     7561 tcaggcgata agcccgccgt cccgtgtgga aggcatgccc gactgtgccg ttgagttgga
     7621 ttgcaccatc agcccgaaag tgagccatcg cgtgaaggaa aagccccagg tctccaaggt
     7681 cgtcctcgcc tattcaggcg ggctcgatac ctcgatcatc atcccctggt tgaaagagaa
     7741 ctacggctgc gaagtgatcg ccttcgccgc caacgtcggt caggccgacg agcttgatgg
     7801 gcttgaggag aaggcgctcg cctcgggtgc atccaagctc tacgtcgagg atctgcgcga
     7861 ggagttcctg aagcagtacg cgttcccgac actgcgggcc ggcgctgttt acgagcgcaa
     7921 gtacctgctt ggcaccagcc acgcgcgtcc gatcatcgcc tcgcatcagg tcgctgtcgc
     7981 caagctggag ggggccgacg gtctctgcca cggctgcacc ggcaagggca acgaccaggt
     8041 tcgcttcgaa ctgacctacc aggcgctggc gccggagatg gccgtcatcg ctccttggcg
     8101 tgaatggagc atcgagtctc gcgaggacgc gatcgactac gctcgcatgc acgagattcc
     8161 gatccgcgcc tcgcgagaga atatctacag tcgcgaccgc aacctctggc acttctcgca
     8221 tgagggcggc cagctcgaag atccgtggct tgagccgccg gccgatctgt tcggcatctc
     8281 cgtctcgccc gaagaagctc ctgacacgcc acacgagttg acaatcagct tcgatgaggg
     8341 ctggccggtc gccctcgacg gccagcagct tggcgcggtc gaactgctga atcacctcaa
     8401 tgaggttggc gggcagcacg gcatcggccg gatcgatctg gtcgagaacc ggctggttgg
     8461 catgaagagc cgcggcgtct acgagcagcc gggcgccacg atcctttcga cggcccacaa
     8521 ggagctcgaa gcgatcacgc tcgaccgcca gacgatgcac tacaaggatc tcgtcgccca
     8581 gcgctacgcg gaactggtct acgatggcct ctggttcacg ccgctgcgcg aggcgctgga
     8641 cgcattcgtc gatgtcaccc agaagcgcgt caccggcgac gtcaagatta agctctacaa
     8701 gggcaactgc atcatcgccg gtcgccgctc gccgtactcg ctctactcag agacgtttgc
     8761 gaccttcggt gcgagtgccg tctacgatca tgccgacgcc gccggattca tcaccctctt
     8821 cggcttgccg ctcaagatca cagccctcag ccaggctgcc ggaagcgagg actagcaggc
     8881 gccacccatt cgtacgatcg acgcgcggcc ggccggcgcc ggccgctctt gttgcaccac
     8941 gaatcgtcat tcagttacgc ttcgcactcg ggtagatgat ctatcacttc gccagcacca
     9001 agggagtcaa ccaggtggcc aggaagtcac agtcccagtc tcgccctgcc cggaaggcag
     9061 ccgggaacat ccagtcgtca agccaaccag aaacctcgac gccaaagctc gacccgctct
     9121 cgcagcggag agagacccgt tcgcggggtc ggagcgcagc agcagaggca cgccgccgcc
     9181 agaagcgcat ccgcaacatc gtgctcgccg tcgttgcggt cctggcgatc gggctaggca
     9241 gcgtcttcgc ctatcaacgc atcacctacg tcgagccggg cacgaaagcc gccgacctcg
     9301 gcggcggtca cgtcgagcaa ggcacaaccg tcagcgacta caacacggat ccgccaactt
     9361 ccggtcagca ctacgcctcg accgctccct ggggtatcca caccgagcca gtccagaacg
     9421 aactccaggt tcacaacctc gagcacggcg ggatcgtcat ccagtacaac gacagcctgt
     9481 cagctgacga gatcagcgtc ctggagagca tcaccaacca atgcgacgtc aagctgctgc
     9541 tcgctccgcg cccggacatg gagcagcgca tcgccgtgac ggcctggacc tactacgagt
     9601 atttcgacac agccgaccgc gatgcgatcc agaagttcat cgacgcccac gtcgacgagg
     9661 gccccgagcg catcgccagc gaatccgacc gctggaacga ctgcaactaa tccctgtcat
     9721 ccaccgtcca gagggcaccc ggcagcgaag gacctctctt cgtc
//
LOCUS       DASUGJ010000402         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_757001, whole genome shotgun sequence.
ACCESSION   DASUGJ010000402 DASUGJ010000000
VERSION     DASUGJ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757001"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VFV93_08840"
     CDS             <1..948
                     /locus_tag="VFV93_08840"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5165487.1"
                     /translation="DGPVTSPDNLAALYAFTRHFAADPRVVRIDSLVTLDPRISLAQY
                     QMLYRDPNAIPDRFVAMNYNELAGEHATAVLLYTRGLAGDDASKALLADIRAYQVGGD
                     YDVLVDGGTAEIVDVVNEMYAEFPPVAAVIILATYLVLLFLFRSAVLPLKAICMNILS
                     IGASYGALVFIFQEGHFSGLLGFEPLGYVEASLPILMFCVLFGLSMDYEVFLLSRVRE
                     AYLETHDNRTSVALGLQGSGRIITGAALIVVVVTASFVSAEIVLVKALGLGIAIAVLL
                     DATIVRALLVPATMRLLGDINWWLPTPVRRLLPRREFEH"
     gene            945..2051
                     /locus_tag="VFV93_08845"
     CDS             945..2051
                     /locus_tag="VFV93_08845"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX5165488.1"
                     /translation="MRIRVWVVVFLPVALLLLAACGSSVDPAPPSSAPTPTSAAAAYL
                     TPEPAIAPIDFPEDEAPHEMLTEWWYYTGHLFTADGTRYGFEYVIFQARRGAFPPYFA
                     GHFAITNSAADEFTYAEKFGIDAALPTDAGFSFDLDGWTMRGALGEDALSATMDRYAI
                     DLQLSSTKPPAFHDGNGYVEFGPSGGSYYYSRTRIDVSGTLTVDGEPLPVTGEAWFDH
                     QWGNFITVGAGGWDWFSVQLDDGSDLTISLVRNDDGSLATAYGTVVAPDGTQRNLTAA
                     DIAVTTEATWTSSDTGTTWPAGWTITLPNDGWTLTVTPTMADQELQTLASTDTIYWEG
                     EVRIDGTANGAPATGLGYVELTGYANGLPIQLGQ"
     gene            complement(2298..>2497)
                     /locus_tag="VFV93_08850"
     CDS             complement(2298..>2497)
                     /locus_tag="VFV93_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165489.1"
                     /translation="DPTFVKLTDPVAELDLAGPALTVPNPVPPGKYIIDITIHWPIPA
                     DIAQLAPSEAKTEYVFVVVVP"
BASE COUNT          393 a          899 c          746 g          459 t
ORIGIN      
        1 gacggcccgg tcacttcacc cgacaacctg gcggccctct acgcgttcac ccgccacttc
       61 gccgccgacc cgcgcgtcgt gcggatcgac agcctcgtaa cgctcgatcc gcgcatctcg
      121 ctggcgcagt accagatgct ctaccgcgat cccaacgcga tccccgaccg cttcgtcgcg
      181 atgaactaca acgaactggc cggcgagcat gcgaccgccg tcctgctcta cacacgcggg
      241 ctggcaggtg acgatgcctc gaaagcgttg ctggccgaca tccgcgccta ccaggtcggt
      301 ggtgactacg acgtcctcgt cgacggcgga acggccgaga tcgtcgatgt cgtcaatgag
      361 atgtacgccg agttcccacc ggtcgccgcc gtcatcatcc tcgccaccta cctcgtcctg
      421 ctcttcctct tccgctcggc cgtcctgccg ctcaaggcga tctgtatgaa catcctctcg
      481 atcggcgcct cgtatggtgc gctggtcttc atcttccagg agggtcactt ctccggcctg
      541 ctcggcttcg agccgctcgg atacgtcgaa gcctcgctgc cgatcctgat gttctgcgtc
      601 ctgttcggcc tcagcatgga ctacgaggtc ttcctgctct cccgtgtccg cgaggcgtac
      661 ctggagaccc acgacaaccg caccagcgtc gcgctcggct tgcagggtag tgggcggatc
      721 atcaccggcg cggcgttgat cgttgttgtc gtgactgcgt cgttcgtctc ggccgagatc
      781 gtcctcgtca aggcgctcgg cctcggcatc gcgatcgccg tcctgctcga cgccacgatt
      841 gttcgcgccc tgctcgtccc ggccacgatg cgcctgctcg gcgacatcaa ctggtggctc
      901 cccacccccg tccgccgcct gctgccgaga cgggagttcg agcattgagg attcgtgtct
      961 gggtcgtcgt tttcctgcct gtcgcgctcc tcttgctggc ggcctgcggc tcgtccgtcg
     1021 atcccgcgcc gccgtcgagt gcgccgacgc cgaccagcgc cgccgccgcc tacctgacgc
     1081 cggagccagc gatcgcgccg atcgacttcc cggaggacga ggcgccgcac gagatgctga
     1141 ccgagtggtg gtactacacc ggccacctct tcaccgccga cggcacccgc tacggcttcg
     1201 agtacgtcat cttccaggcc cggcgcgggg cgttcccgcc ttacttcgcc ggccacttcg
     1261 cgatcaccaa cagcgccgcc gacgaattca cctacgccga gaagttcggc atcgacgccg
     1321 ccctcccgac cgacgccggc ttcagcttcg atctcgacgg ctggacgatg cgtggtgcgc
     1381 tcggcgagga tgcgttatcg gccacgatgg accgctacgc gatcgacctg cagctctcat
     1441 cgaccaagcc gcccgccttt catgatggca atgggtacgt cgaattcggc ccatccggtg
     1501 gctcgtacta ctactcgcgg acacgcatcg acgtcagcgg cacactgacg gttgatggcg
     1561 agccgttgcc ggtcaccggc gaggcctggt tcgaccacca gtggggcaac ttcatcacgg
     1621 tcggcgccgg tggctgggac tggttctccg tccagctcga cgacggaagc gacctgacga
     1681 tctcgctcgt tcgcaacgac gacggctcgc tcgcgaccgc gtacggcacc gtggtcgccc
     1741 cggacggcac gcagcgtaac ctcaccgccg ccgacatcgc cgtcacgacc gaggcgacct
     1801 ggaccagctc cgacaccggc acgacctggc ccgccggctg gacaatcacg ctccccaatg
     1861 acggctggac actgaccgtc acgccgacga tggccgacca ggaactccag acgctcgcca
     1921 gcaccgatac gatctactgg gagggcgaag ttcgcatcga cggcaccgcc aatggcgccc
     1981 ccgccaccgg cctcggctac gtcgagctga ccggctacgc caatggcctg ccgattcagc
     2041 tggggcagta attgcattcg cgacttcgca tccagaggga gatgctgcac gaggagcgta
     2101 ccccttgccc tgtcatcctg agccgcagcg aaggatcgcc tggccatgac gacgtgatcg
     2161 gttcggacgg gtcatcaggg ttctaccgat cggcgagctc cggtcgttca cgttcagcga
     2221 tccttcgcta cggctcagga tgacaggggt gaggcgtgcg gtcctcgcca acgccgtccg
     2281 tctgggtcgc tgaatcgtta cggcaccacc acgacaaaga catactccgt cttcgcctct
     2341 gacggagcca gttgagcgat gtcggccggg atcggccagt ggatcgttat gtcgatgatg
     2401 tacttcccgg gtgggaccgg attcggcacc gtcaatgccg gccctgccag atccagttcc
     2461 gcgaccgggt cggtcagctt gacgaacgtc ggatcag
//
LOCUS       DASUGJ010000403         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_340428, whole genome shotgun sequence.
ACCESSION   DASUGJ010000403 DASUGJ010000000
VERSION     DASUGJ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340428"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFV93_08855"
     CDS             <1..232
                     /locus_tag="VFV93_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165490.1"
                     /translation="ASQGTEPPPARFSTQIRILGHVSLAQPQSTLSPRHDHRSAADHR
                     AYPGVMMYQYGMGSWAARETGSQWKHVRRLIC"
     gene            202..1455
                     /gene="hutI"
                     /locus_tag="VFV93_08860"
     CDS             202..1455
                     /gene="hutI"
                     /locus_tag="VFV93_08860"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX5165491.1"
                     /translation="METRPKADLLIDNAGELLTCADGAADLIGIVPGGVVAVAGERIV
                     AVGTAAEVAAAVDAEGARRIDAGGQVVMPGFVDCHTHVVFGGSRVEESAAKVSGGALE
                     AVRERGAPVGIVGTVAWTRALGVDGLADVTLPRLREMLAHGTTTVESKSGYGLTVEAE
                     LDMLRANRRLAGLQPVDLVSTFLGAHAIPPELSREQYVELILSEMLPRVVDEGLAEFH
                     DVWCEQGYFTVEESRRLLEAGLRLGLKPKLHLDQNSHTGAAALAAEVRATSVDHLNFT
                     PLAEMRLMAGAGVTAVAMPGIDFATAHTPPVDCRRIIESGMTLALATDICPGGWIPSM
                     QLIVAFACRLHGLSPAEAIRAATLGAARAVARQDEIGSLEAGKLADILILDIERHEDM
                     AYKIGRNAVRTVIKRGQVVVERSES"
     gene            1452..2870
                     /locus_tag="VFV93_08865"
     CDS             1452..2870
                     /locus_tag="VFV93_08865"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX5165492.1"
                     /translation="MSDRQYLRAAHLHQPDGWLSPGFLEIDGDGTISQVTATMPAEWD
                     ATEVQRLDGYVLPGMLNLHSHAHQRGLAGRAEGGSGATEAENFWSWRERMYAFLLSLT
                     PDDFEAIAAQLYLEMLRAGFTTVGEFHYLHHDRDGGYYADPHEMSARLLAAADQTGIA
                     LTMLPSLYTHAGIGRPATPEQRRFVHRSVDDFLALFEWLRTEMPNRPLLRVGVAPHSL
                     RAVSGEELSAVVASVSGLPDVPIHLHVAEQTGEVEEIVTRLGTTPASWLFANQPVNDR
                     WTFIHATHCTLDELRELAGRGAVVGLCPTTEGNLGDGLFQLRDYAAAGGRWGIGTDAN
                     LQPDPGLELRFLEYGQRLFSQRRSILVEPGATTAQPGRRLHDLALAGGAQALAQPAAA
                     LTAGRRADLVELDPDHPAIVAQQPASVLDGWLVAGSRELVRTVLVGGTWLVRDGHHQH
                     EETITDRYRAVMRRLAGAALSS"
BASE COUNT          436 a          970 c         1035 g          436 t
ORIGIN      
        1 agccagtcaa ggcaccgaac ctccacccgc caggttttcc acccaaatcc gcatcctcgg
       61 tcacgtgtcg ctcgcccaac cccaatctac cctctcgcca cgacacgatc accgcagcgc
      121 tgcggatcat cgcgcctatc cgggcgtgat gatgtatcaa tacggcatgg gatcgtgggc
      181 tgcgcgggag acgggatcac aatggaaaca cgtccgaagg ctgatctgct gatcgacaac
      241 gccggtgagc tactgacctg cgcggatggt gcggccgacc tgatcggcat tgtgccgggc
      301 ggggtcgtcg ccgtcgccgg cgagcggatc gtcgcggttg ggacggcggc ggaggtcgcg
      361 gcggcggtcg atgcggaggg tgccaggcgg atcgacgcgg gagggcaggt cgtcatgccc
      421 ggcttcgtcg attgtcacac gcacgtcgtc ttcggtggct cgcgggtgga ggagtctgcc
      481 gccaaggtca gcggcggcgc tctggaggcc gttcgcgagc gcggagcgcc ggtcgggatc
      541 gtcgggacgg tcgcatggac gcgggcgctc ggcgtcgatg ggctggctga cgtaacgctg
      601 ccacggctgc gcgagatgct ggcgcacggg acgacgacgg ttgagagcaa gagcggctac
      661 gggctgacgg tcgaggccga gctggacatg ctgcgcgcca accggcggct ggccgggctc
      721 cagccggtcg atctcgtcag caccttcctc ggggcgcacg ccatcccgcc cgagctgtcg
      781 cgcgagcagt acgtcgagct gatcctgagt gagatgctgc cgagggtggt cgatgagggg
      841 ctggccgagt tccacgatgt ctggtgcgaa cagggctact tcacggtcga ggaatcgcgg
      901 cggctgctgg aggcgggact gcgcctgggg ctgaagccga agctgcacct cgaccagaac
      961 agccacaccg gcgcggcggc gctggcggcc gaggtgcggg cgacgtcggt cgatcacctc
     1021 aacttcacgc cgctggctga gatgcggctg atggcggggg cgggcgtgac ggccgtcgcg
     1081 atgccgggga tcgacttcgc gacggcgcat acgccgccag tcgattgtcg ccggatcatc
     1141 gaaagtggga tgacgctggc gctggcgacc gacatctgtc ccggtggctg gattcccagc
     1201 atgcagctga tcgtcgcctt cgcctgccgg ttgcatggcc tctcgccggc cgaggcgatc
     1261 cgggccgcga cgcttggcgc ggcgagggcg gtggcccgcc aggacgagat cggctcgctc
     1321 gaagcgggca agctggccga tatcctgatc ctcgatatcg agcgccacga ggacatggcc
     1381 tacaagatcg gccggaacgc tgtccggacc gtcatcaagc gcggccaggt cgtcgtcgaa
     1441 cggagcgagt catgagcgac cggcagtacc tgcgcgcggc ccatctgcac cagcccgacg
     1501 gctggctgtc gccggggttt ctggagatcg atggcgacgg cacgatctcc caggtcacgg
     1561 cgacgatgcc ggccgagtgg gacgcaaccg aggtccagcg gctggatggc tacgtgctgc
     1621 ctggcatgct caacctgcac tcgcacgccc atcagcgggg gctggcggga cgggccgagg
     1681 gcggttccgg cgcgaccgag gcggagaact tctggagctg gcgcgagcgg atgtacgcct
     1741 tcctgctcag cctgacaccg gacgacttcg aggcgatcgc ggcccagctc tatctcgaga
     1801 tgctgcgggc aggcttcacg acggttggcg agttccacta cctgcaccac gaccgcgacg
     1861 gcggctacta cgccgatccg cacgaaatgt cggcgcggct gctggcggca gccgaccaga
     1921 ccgggatcgc gttgacgatg ctgccctcgc tctacaccca tgccgggatc ggccggccgg
     1981 ccacgccgga acagcgccgc tttgtccacc gctcggtcga tgattttctg gcgttgttcg
     2041 agtggctgcg gaccgagatg cccaaccgcc cgctgctgcg ggtcggcgtc gcgccgcata
     2101 gcctgcgggc agtcagcggt gaggagctat cggccgtcgt cgcgtccgtc agtgggctac
     2161 cggacgtgcc gatccacctg cacgtcgccg agcagaccgg cgaggtcgag gagatcgtca
     2221 ccaggctcgg gacgacaccg gccagctggc tgttcgccaa ccagccggtc aacgaccgct
     2281 ggaccttcat ccacgctacc cactgtacgc tcgacgagct acgcgagctg gccggtcgcg
     2341 gcgccgtcgt cggcctctgc ccaacgacgg aggggaacct cggcgacggc ctcttccaat
     2401 tgcgcgacta cgctgctgcc ggcggccgct gggggatcgg caccgacgcc aacctgcagc
     2461 ccgatccggg gctggagttg cgcttcctcg aatacggcca gcggctgttc agccagcgcc
     2521 gcagcattct ggtagagccg ggcgcgacga ccgcgcagcc cggccggcgc ctccacgacc
     2581 tggcgctggc cggcggcgcc caggcgctgg cccagccagc cgctgcgctg accgccggcc
     2641 ggcgcgctga cctggtcgag ctggaccccg accatccggc gatcgtggcc cagcagcccg
     2701 ccagcgtcct cgacggctgg ctggtcgccg gctcccgcga actggtccga accgtcctcg
     2761 tcggcggcac ctggctcgtc cgcgacggcc accaccagca cgaggagacg atcactgatc
     2821 ggtatagggc ggtgatgcgc cggctggcgg gggctgccct gtcgtcctga gccgcag
//
LOCUS       DASUGJ010000404         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1868205, whole genome shotgun sequence.
ACCESSION   DASUGJ010000404 DASUGJ010000000
VERSION     DASUGJ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868205"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VFV93_08870"
     CDS             complement(<1..1190)
                     /locus_tag="VFV93_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5165493.1"
                     /translation="MSRFLDDVEQLRTDVIAGRMSRRDVLKRGAMLGLSAPIIAGLLA
                     ACGSDDDDDDDTTDAPTSTTGAAVATNTTASSGGGEATATTGGGGSAASPTTSQDVEP
                     TETTAPAAEAGGGGLLRLLWWQAPTIINPHLATGTKDFDASRVCLEPLADFDVDGNGI
                     PYLAAEMPSLENGGVNEEGTQVTWKLREGVKWHDGEDFTADDVVFTWQYASNEETAAV
                     TQGNFANVESVEAVDDYTVTITFSQPNPAWFDVFTGPNGMILPEHVLKDAIGAGARDA
                     PFNLMPIGTGPFMVSEFRAGDVVTYDRFPDYWDAGKPYFDSVEMKGGGDAPSAARAVV
                     VTGEADWAWNIQVEPEILQQMEGEGGPGATQTTPGASAERMMVNFADPNAEVNGARSE
                     PSTQ"
     gene            complement(1324..>2012)
                     /locus_tag="VFV93_08875"
     CDS             complement(1324..>2012)
                     /locus_tag="VFV93_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5165494.1"
                     /translation="IQRDTIAEQLYGPGGVAASNQYDEPAKFSNPNITWEFDLDKAKA
                     LLDEMGFEGGDILYQTSINTVRQKTQEIVKQDLAKLGFNVEIKSVDAAVYCSTDQGSP
                     DTSGKFYADLEMHTNGPTIYPATWFERYRTDEIAEQANGWSGSNYFRYSNPEMDKMHD
                     QVRIEMDPDTQVELFQKMLELSTVIDYVEVPVVNRTGLAAKSKKIQGYVGSPWSSNPV
                     YEIKNWTMSE"
BASE COUNT          305 a          672 c          635 g          400 t
ORIGIN      
        1 tgctgcgtgc tcggctcgga gcgcgctcca ttcacctccg cgttcgggtc ggcgaagttg
       61 accatcatcc gctcggcact ggcgccaggc gtcgtctgcg tcgcgcctgg tccgccttcg
      121 ccctccatct gctggaggat ctccggctcg acctgaatgt tccaggccca gtcagcctcg
      181 ccggtgacaa ctacagcccg ggcagcggac ggtgcgtcac cgccgccctt catctcgacc
      241 gagtcgaagt acggcttgcc ggcgtcccag taatccggga agcggtcgta cgtgacgaca
      301 tcgccggccc ggaactcgct gaccatgaac ggtccggtcc cgatcggcat caggttgaat
      361 ggcgcatcgc gcgcgccagc cccgatcgca tccttcagga cgtgctccgg caggatcatg
      421 ccgttcggcc cggtgaagac atcaaaccag gccgggttgg gctggctgaa cgtaatggtg
      481 accgtgtagt cgtcaaccgc ctcgaccgac tcgacgttgg cgaagttacc ctgggtcacg
      541 gccgctgtct cttcgttgga cgcgtactgc caggtgaaga cgacatcgtc ggcggtgaag
      601 tcctcgccgt cgtgccattt gacgccctca cgcagcttcc aggtgacctg ggtgccttcc
      661 tcattgaccc cgccgttctc cagcgagggc atttcagccg ccaggtacgg gatgccgttg
      721 ccatcgacgt cgaaatcggc cagcggctcc agacagaccc gcgaggcgtc gaagtccttc
      781 gtgccggtcg ccaggtgcgg gttgatgatc gtcggcgcct gccaccagag cagccgcagc
      841 agcccgccgc cgcccgcttc cgctgccggg gcggtcgtct cggtcggctc cacgtcctga
      901 ctggtggtcg gcgaggcagc cgaaccgcct ccgccggtcg tcgcggtcgc ctcgccacct
      961 ccgctggagg cggttgtatt cgtcgccacg gccgcgccgg tcgtgctggt tggcgcatcc
     1021 gtggtgtcat cgtcatcgtc gtcgtccgag ccgcaggcgg ccagcagccc ggcgatgatt
     1081 ggcgcgctca ggccgagcat tgcgccccgt ttgaggacat cgcgacggga catcctgccc
     1141 gcgatcacat ccgttcgtag ttgctcaacg tcgtccagga atcggctcat agccgcatct
     1201 cctctcgtcg aacatgcctc gatgtccttc gcgccacggt ccagcgtggc gcagagtgcc
     1261 atgaacggat cgcggccacc tcgaccagat ggtcgccggt ggccgcgaga gccgcgccgg
     1321 atgttactcc gacatcgtcc agttcttgat ctcgtagacc gggttcgacg accacggtga
     1381 gccgacgtag ccctggatct tcttcgactt ggccgcgagg cccgtccggt tcacgaccgg
     1441 gacctcgacg tagtcgataa cggtcgacag ttccagcatc ttctggaaca gctcgacctg
     1501 cgtatccgga tccatctcaa tccggacctg atcgtgcatc ttgtccattt ccggattgga
     1561 gtagcggaag tagttggagc ccgaccagcc gttggcctgc tcggcgatct catcggtccg
     1621 atagcgctcg aaccaggtcg ccgggtagat cgtcggaccg ttggtgtgca tctcgaggtc
     1681 ggcgtagaac ttgccggagg tatccggcga gccctggtcg gtcgagcagt agacagcggc
     1741 atcgacgctc ttgatctcga cgttgaagcc gagcttggcc aggtcctgct tgacgatctc
     1801 ctgcgtcttc tgccgaacgg tgttgatcga ggtctggtag aggatgtcgc caccctcgaa
     1861 gcccatctcg tccagcaggg ccttggcctt gtcgagatcg aactcccagg tgatgttcgg
     1921 gttcgagaac ttggccggct cgtcgtactg attcgaggcc gccacgccgc cgggaccgta
     1981 gagctgctcg gcgatcgtgt cgcgctggat cg
//
LOCUS       DASUGJ010000405         9135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_61944, whole genome shotgun sequence.
ACCESSION   DASUGJ010000405 DASUGJ010000000
VERSION     DASUGJ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61944"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..641
                     /locus_tag="VFV93_08880"
     CDS             105..641
                     /locus_tag="VFV93_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165495.1"
                     /translation="MSIFQRFTHHHAPQVMVIDPAVLPRFRYPNGVFAVLTTTPDDVA
                     DRSYEAFRAYHLTPGVAEREVAKLLMGAEPVCLPVESSPGYAYEVGRERTDLGRMRVF
                     AHYLVRMQLTHESNQQQLLAIREIHHARRLRILPGCCEVCDKLAGRTWRVADPPELPV
                     RGCLRQGGCRCSYVPEVD"
     gene            complement(1054..1698)
                     /locus_tag="VFV93_08885"
     CDS             complement(1054..1698)
                     /locus_tag="VFV93_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5165496.1"
                     /translation="MTVDILVVDDHPIVREGMVAILESQADFNVVAEAGNGAEALELY
                     KRHSPNVVIMDLQMPGTDGVAGIQGIRAYDADAKIVVLTAYDTDERILQAVQAGARGY
                     LLKGAPRDDIFRAVRVVNLGGSLLEPAVAGKLLSHVGGIMRGENKDDELTSRELDVLR
                     LMSKGLRNKEIATELFITERTVKFHANSIYQKLDVNGRTEAVSRAIQRGLVTLS"
     gene            complement(1858..4569)
                     /locus_tag="VFV93_08890"
     CDS             complement(1858..4569)
                     /locus_tag="VFV93_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5165497.1"
                     /translation="MDARHHADRQAVAGLLREHAESIHAAWYRVIVEQNPNAAQQSLL
                     LPEFLDALFSYVADPDPAVPAGVAATWCQQVDPTTAGMVSTAVAMGLLGEALRQGLPD
                     FESLAVQNAYGMVTVALPDFSAEFVRGLMSSGEMPTNEQHWYEVSQRLAAERTHRLKQ
                     LAILNDVSMALSSTLSLDEVYEIIYQQASRLVDTSNFYIATLGTAPGEMTRRLHIFGG
                     ARDSLDPDAPIRMGLTKEVVRESKLVIVDDYVVACRERGLPITSEIDQTKTLAWLGAP
                     IVAGDRPIGVLVVMTENGPFDRDDAEILAAVARQAGGAMANARLFEAQRSQASQLRAI
                     NQISRAISTIREPDVLMQTSCTLIRDLFGYSVVSILLAHPDNDLLVLRTQSGLEGDGI
                     VGYSMQIGGKGLIPHAAATCQPVLVNDVTRDARYVVTRYTGHIRSEVSIPLLRDGRLV
                     GVLDLQSPHIDAFSEADVELLTTVGEQLTVALENAELLVQERKRRTELALILEVSQAA
                     NSSLVLDDVIQRVAEGISDAVGLPSCIVYLYDDEGDRLLPSAFVARDGSELDTSRISK
                     VIPNTESSQLLRRIFLSGQEASAIEMLTCDVDDELAQVLSATAVLAVPFVAKQQFLGF
                     ALLVAHDAEYHFSPHQLRVAYGVAGAAALALENARLYARSHTLGMAEERIRVAREIHD
                     GMAQGLTAVALNLEAADQLFEIKPVKSREKINRALELTRSNLEDARRSVLDLHASALQ
                     ELTLTEALQRRTQQFLDEHREQQVTGVFNGESMYGRLSSRMELSLYRIFEEALDNVAR
                     HANATHIDVSIARGGDDVVLAVTDNGHGFDVEQALSARRTPGTFGLVAIRERVRLLHG
                     TMEVHSADGQGTTMIVTVPYEAQHSGAKAPKSLATPASPVVLAGDGG"
     gene            4930..6666
                     /locus_tag="VFV93_08895"
     CDS             4930..6666
                     /locus_tag="VFV93_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165498.1"
                     /translation="MFRALLVAIGVVVVVSTLPVNPSQPSAFADPAFSRYYARSSAAE
                     QQLLWGGGPLVSLVEPFTGAPGNRRLVQYFERGRMEMADSTAGTSAGAISQGLLVREM
                     ATGYVQLGYDDFVQGEPAPIPLFGPSDADALTYADFAVPVATKAADRTGELLDDWIAP
                     GGVISKATPPAEILAGGYEPVTGHNIPHVTASWLKTDPFGIDANDALGLPISEPYWVH
                     SGKGESGISLIQLFERRVVVYTPNLPLAERFSLTSAGRHYYRWRYGNDPGADTADAPA
                     QREVLARADDTAGLSLPDGYQASILSDAADAFGIAVTPNGKLAVGYNDGRIELIDPRD
                     PTAKPTTFVEHLANPAVFTWAGTDLYVVDDSGLHRYHDRDANGVADDDETIIDGSFER
                     ASVQLAPGPDATLFLTGRPTGATGTAFAESGAPQLYQIAADALTTTDSAPAALDTTAV
                     NALLVDDSGAVWLIDDNDQLVQYLPETSGERTLLDASQFASVRDVLLYRPDGTSGNPY
                     RDMLALADGRIVRLQPSRPDSASPSAGTGPAAIVDFITGFDRPTAMVSGLDGSLYVLD
                     TGRDVIYQIKPD"
     gene            6797..7609
                     /locus_tag="VFV93_08900"
     CDS             6797..7609
                     /locus_tag="VFV93_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX5165499.1"
                     /translation="MAIRIFTVDAFTHRPFSGNPAGVCLLDDAPDLPETGWMQAVAAE
                     MNLSETAFLRRGSDGRAFHLRWFTPVVEVPLCGHATLASAHVLIEEGILPGDQPAAFD
                     TLSGRLTARRAENGIEMDFPLHPVVPLADDAVPDALREGLGVPLQHVTADSNRYVVEL
                     ADEATLRELQPNLALLSTLMRAVVITCRSETPEFDFVSRNFLPHRGIPEDPVTGGAHC
                     SLADYWRSRLGKETFTAYQASRRGGALGVRITGDRVILTGQAVTVLRGTLQA"
     gene            7655..8875
                     /locus_tag="VFV93_08905"
     CDS             7655..8875
                     /locus_tag="VFV93_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5165500.1"
                     /translation="MATAIDFSDPAIQANPYPVYRQLREESSVVWNETTSSWLISRYD
                     DIVALLNDPRISSARVDATFRVLPEDVQRELQPLRDVLSQRMLLTDPPTHTRLKNLVM
                     PAFAARMSERRRARIQEICDSFIDRLAEADTFDVMRDFATPLPGWVTGDVLGVPVERQ
                     QQFSDWSHDQVRIYDRLGTVHDRIAVMRQGQASMLAMKAYLEEIIEQRRRLPGDDVIT
                     MLVQAEEAGDTLTNDEMVVMVVAILVGGNNSTAHLIGNAILTLLRQPEALARLRDDPA
                     LIRPAIEEVMRFESPVQSTSRVAKEPIEIGGQTIEPGNNVHVLLASGNRDEAQFPTPD
                     TFVIDRSPNRHLTFAHGPHYCLGTSLARTMTQTAVQTLLTRFPDLRLASDTPEWNEGF
                     SFRSLKTLPVVSAM"
BASE COUNT         1574 a         3186 c         2861 g         1514 t
ORIGIN      
        1 gaccggcttc ctgttaaaaa ggacatacgc atcgtgtgcg aagtacaaac gcacctgccc
       61 gatcgcggtt gagccactcc cgcgaggagc gacaggagat caggatgtcc attttccagc
      121 gtttcacgca ccaccacgcg ccgcaggtca tggtcattga tccggccgtg ctgccgcggt
      181 ttcgttaccc gaacggcgtc ttcgcggtcc tgacgacgac ccctgacgac gtcgccgatc
      241 gcagctacga ggcattccgc gcctaccacc tgacacctgg cgtggccgag cgggaggtcg
      301 ccaagctgct gatgggtgcc gagccagtct gcctgccggt cgagtcgtcg ccgggctacg
      361 cctacgaggt cggccgcgag cgaaccgacc tgggccgcat gcgcgtcttc gcccactacc
      421 tcgtcaggat gcagctgacc cacgagtcga accagcagca gttgctcgcc atccgcgaga
      481 tccatcatgc gcgcaggctg cgcatcctgc ccggctgctg cgaagtctgc gacaagctgg
      541 ccggtcgcac ctggcgcgtc gccgacccac ccgagctccc ggtccgtggc tgcctccgcc
      601 agggcggctg ccgctgctcg tacgtgccgg aagtggattg agggaatcgg ggcgcggggt
      661 tcgtgacgcg tgacgcgtcc ccgcgtagcg gacatcatct gcatcacgca tcacgtgcgc
      721 agctccgacg cagtgacgag cgttcttggt tgatactggt acgtggacgc tggcggaagg
      781 tcgtgttgga tcccgcccat cccggttcag atcccgagaa ccggcagtta cggcaccccg
      841 cctgatcgcg acattcccca cagggcgggc actaccgtgg acggacacaa ctctactggc
      901 cccaccacgt cacaccggga cggccggatc accgggaacg ctcgtcactg cgccggatac
      961 gacgtaggtg ttaggtgtta ggggctggtg tgtgaaaagg gacgtatcgt ctcgactccc
     1021 acgcaccagc ctctaacacc taacgcctat cacctacgac agcgtcacca acccgcgctg
     1081 gatggcccgc gacacggcct cggtccggcc gttaacgtcg agcttctggt agatcgagtt
     1141 ggcgtggaac ttgaccgtcc gctcggtgat gaacagctcg gtcgcgatct ccttgttgcg
     1201 cagccccttc gacatcagcc gcaggacgtc cagctcgcgc gaggtcagct cgtcgtcctt
     1261 gttctcgccg cgcatgatcc cgccgacgtg cgagagcagc ttgccggcga cggccggttc
     1321 cagcagcgag ccgcccaggt tgacgacgcg gacggcccgg aagatgtcgt cacgcggggc
     1381 gcccttgagg agatagccgc gcgcaccggc ctggacggcc tggaggatgc gctcgtcggt
     1441 gtcgtaggcg gtcagaacga cgatcttcgc gtcagcatca taggcgcgga tgccctggat
     1501 gccggcgaca ccatcggtgc cgggcatctg caggtccatg atgacgacgt tcggcgagtg
     1561 ccgcttgtag agctccagcg cctcggcgcc gttaccggcc tcggcgacaa cgttgaaatc
     1621 ggcctgcgat tcgaggatcg cgaccatgcc ctcgcgaacg attgggtggt cgtcgacgac
     1681 gagaatgtca accgtcatat tccccaacaa tcctttccca cacaacatcg tggatcgtga
     1741 cagacgttgt cactcctgac cataggctgc tggtcccggg ccggggcatc caacgggcct
     1801 cttcgcccgc tgcctgctca tcgcgataca ggtattcatg tcccatccgc gccaccgtta
     1861 gcccccatct cccgccagga cgaccggcga ggccggcgtt gccagcgatt tcggggcttt
     1921 cgcgccgctg tgctgcgctt cgtagggaac cgtcacgatc atcgtcgtgc cctgaccgtc
     1981 ggctgagtgg acctccatcg tcccgtgcag caggcgtacc cgctcgcgga tcgcgaccag
     2041 cccgaacgtg ccgggcgtcc gccgggccga gagggcctgc tcgacatcga acccgtgccc
     2101 gttgtctgtc acggccagga cgacatcgtc gccaccgcgc gcgatcgaga catcgatatg
     2161 ggtcgcgtta gcatggcggg cgacgttgtc gagcgcctct tcgaagatgc gatagaggct
     2221 cagctccatg cgcgacgaga gcctgccgta catcgactcg ccgttgaaga cgccggtcac
     2281 ctgctgctcg cgatgctcgt cgaggaattg ctgggtacga cgctggagcg cctcggtcag
     2341 cgtgagttcc tggagcgcgc tggcgtgcag gtcgaggacg gaacgacggg cgtcttcgag
     2401 attggagcga gtcagctcca gcgcacggtt gatcttctcg cgggacttga ccggcttgat
     2461 ctcgaagagc tgatcggccg cttcgaggtt cagcgcaacc gccgtcaacc cctgggccat
     2521 cccgtcgtgg atctcgcggg cgacgcgaat acgctcctcg gccataccga gcgtgtgcga
     2581 gcgggcgtag agccgcgcgt tttccagcgc gagcgccgcc gcgccggcca cgccataggc
     2641 cacgcggagc tggtgcggag agaagtgata ctcggcgtca tgtgccacca gcagcgcgaa
     2701 gccgaggaac tgctgcttgg cgacgaacgg caccgccagg acggctgtcg ccgacagcac
     2761 ctgcgccagc tcgtcatcga cgtcgcaggt cagcatctcg atcgccgatg cctcctggcc
     2821 ggagaggaag atgcgccgca gcagttgcga cgattcagtg ttgggaatca ccttgctgat
     2881 ccggctcgtg tccagctccg agccgtcccg cgcgacgaag gccgagggca gcagccggtc
     2941 gccctcgtcg tcgtagaggt agacgatgca cgacggcagg ccgaccgcgt cggagatgcc
     3001 ctcggccacc cgctggataa cgtcgtccag caccagcgac gagttggccg cctgcgacac
     3061 ttcgaggatc agcgccagct cggtccgacg cttacgctct tgaaccagca gttcggcgtt
     3121 ctccagcgcg accgtcagct gctcgccaac cgtcgtcagc agctcgacat cggcctcgct
     3181 gaaggcgtcg atgtgcggtg actgcagatc gagcacaccc accagccgcc catcgcgcag
     3241 caacgggatc gacacctcgg agcgaatatg gccggtgtac ctggtcacca catagcgcgc
     3301 atcgcgcgtg acatcgttga ccagcaccgg ctggcaggtc gcggcggcgt gcgggatcag
     3361 ccccttgccg ccgatctgca tcgagtagcc aacgatcccg tcgccctcca ggccggactg
     3421 cgtccgcagg acgagcagat cgttgtccgg gtgggccagc aggatcgaga cgacgctgta
     3481 gccgaacagg tcacggatca gcgtgcagga ggtctgcatc agcacgtccg gctcgcggat
     3541 cgtcgagatc gcgcgcgaga tctgattgat ggcccgcagt tggctcgcct gactgcgctg
     3601 cgcctcgaac agccgcgcgt tcgccatcgc cccgccggcc tgccgcgcga cggcggccag
     3661 aatctcggca tcgtcacggt cgaacgggcc gttctcggtc atgacaacca ggacgccgat
     3721 cggccggtca ccggcgacaa tcggcgcgcc cagccaggcc agcgtcttcg tctggtcgat
     3781 ctcggacgta atcggcaagc cgcgctcgcg gcaggcgacg acgtagtcat cgacgataac
     3841 cagcttgctc tcgcgcacga cctccttggt cagccccatc cgaatcgggg cgtcggggtc
     3901 gagcgagtca cgggcaccgc caaaaatatg caggcggcgg gtcatctcac cgggcgcagt
     3961 cccaagcgtg gcgatgtaga agttggacgt gtcgaccagc cggctggcct gctggtagat
     4021 gatctcgtag acctcatcga gcgagagagt cgaggagagt gccatcgaga cgtcgttgag
     4081 gatcgcgagc tgcttcaacc ggtgggtgcg ttcggcggcc agccgctgcg atacctcgta
     4141 ccaatgctgc tcgttggtcg gcatctcgcc gctgctcatc agcccgcgca cgaactcggc
     4201 cgagaaatca ggcagggcga ccgtgaccat cccgtaggcg ttctggacgg ctagcgactc
     4261 gaaatcgggc agcccctggc gcaatgcctc gccaagcagg cccatcgcga ccgctgtcga
     4321 caccatcccg gccgtcgtcg ggtcgacctg ctgacaccag gttgccgcaa ctcctgccgg
     4381 tacggccggg tcgggatcgg cgacgtagct gaagagggca tcgaggaatt caggcagcag
     4441 caacgactgc tgggcggcgt ttgggttctg ctcgacgatc acccggtacc aggcagcgtg
     4501 gatcgactcg gcatgctcac gcaacagccc cgcaaccgcc tggcgatctg cgtggtggcg
     4561 tgcgtccatg ccgtcataca tctacaaaac taccgatcca gtgaactctc aacagtaccc
     4621 attcgcgttg gccgctcgtc cccaccgaca gcccgtccaa cggcatcgtt gtcgtcgatg
     4681 ctcttcatat tatggaactt tgacgacgta agccatcgca ccgagggtca ggagcaggct
     4741 gtactaatgg acaggccaac tggacatttc cctcataccg gccatctgcc gggcaatgaa
     4801 ccggacaatt gtcccagttc ctgtggtatt cgcataaccg cagccatcgt ggttcgttgt
     4861 aacgacagga gtggccgtta tcggccgatc cgctgaatgt atcgtcaatg aacatgtttc
     4921 ggacttctgg tgtttcgggc actgctggtc gcaattggtg tggtagtggt cgtctcgacc
     4981 ctgccggtca acccatcgca gccctctgcg tttgccgatc ccgcgttctc gcgctattac
     5041 gcgcgatcgt ccgcggccga gcaacagctg ctgtggggcg gaggcccact cgtctcgctg
     5101 gttgagccat tcactggcgc acccggcaac cggcggctgg tccagtactt cgagcgcggc
     5161 cggatggaga tggccgacag cacggccggc acgagcgccg gtgcgatcag ccaggggctg
     5221 ctcgtgcgcg agatggcgac cggctacgtc cagctcggct acgacgactt cgtccagggc
     5281 gaaccagccc cgatcccgct cttcggcccc agcgacgccg acgcactcac ctacgccgac
     5341 ttcgccgtcc cggtcgccac caaagccgca gatcgcactg gcgaactgct ggacgactgg
     5401 atcgcacccg gcggcgtcat cagcaaggcc accccgccgg ccgagatcct cgccggcggc
     5461 tacgagcctg tcaccggcca taacatcccg catgtcacgg catcctggct gaagaccgac
     5521 ccgttcggga tcgacgcgaa cgacgcgctc ggcctgccga tcagcgagcc gtactgggtc
     5581 cactccggca agggcgagag tggcatcagc ctgatccagc tcttcgagcg gcgcgtcgtc
     5641 gtctacacgc ccaacctgcc gctggccgaa cgcttcagcc tgaccagcgc cggccgccac
     5701 tactaccgct ggcgctacgg caacgacccc ggcgcggaca cagccgatgc cccggctcaa
     5761 cgcgaggtgc tggcccgcgc cgacgatacg gccggcctct cactcccgga cggctaccaa
     5821 gccagcatcc tcagcgacgc ggccgacgcc ttcgggatcg ccgtcacacc gaacggcaag
     5881 ctggccgtcg gttacaacga cggccgcatc gagctgatcg acccgcgcga cccaaccgcc
     5941 aaaccgacca cgttcgtcga gcatctggcc aatcccgccg tcttcacctg ggcggggacc
     6001 gacctctacg tcgtcgacga cagcggcctc caccgctatc acgaccggga tgccaacggc
     6061 gttgctgatg acgacgagac gatcatcgac ggcagcttcg agcgcgccag cgtccagctc
     6121 gctccaggcc ccgacgccac tctgttcctg accggccgcc cgacaggcgc caccggcacg
     6181 gcgttcgcgg agagcggcgc accgcagctg tatcagatcg ccgccgacgc cctgacaacc
     6241 accgacagcg cgccggccgc gctcgacacg accgccgtca acgccctgct cgtcgacgac
     6301 agcggcgcgg tctggctgat agatgacaac gatcagctcg tccagtacct gccggagacg
     6361 tccggcgaac ggaccctgct ggacgcctcg cagttcgcct ccgtccgcga cgtcctcctc
     6421 taccgtcccg acggcacctc cggcaacccc taccgggaca tgctggcgct ggccgacggg
     6481 cgcatcgtcc gcctccagcc ctcacgcccc gatagcgcct cgccgtccgc cggcaccggc
     6541 ccggccgcca tcgtcgactt catcaccggc ttcgaccgcc cgaccgcgat ggtcagcggc
     6601 ctcgacggct ccctctacgt cctcgacacc ggccgcgacg tgatctacca gatcaaaccc
     6661 gactgattcc agacccttca ccaccatctc cggcctctaa tcggctatgc tgtgcggaat
     6721 ctgaaacaga ttgcgcccgg gtatcacaac cgatccacgt tccggctggc cgttggcaag
     6781 aagggttcac tccggcatgg cgatcaggat cttcaccgtc gacgcgttca cgcatcgccc
     6841 gttctccggc aacccggccg gcgtctgcct gctcgacgat gctcccgatc tgcccgaaac
     6901 cggctggatg caggcggtcg cggccgagat gaacctgtcc gagacggcgt ttctgcgccg
     6961 tgggagtgac ggccgcgcgt tccacctgcg ctggttcacg ccggtcgtcg aggtcccgct
     7021 ctgcgggcat gccacgctgg ccagcgcgca tgtccttatc gaagaaggca tcctgccggg
     7081 ggatcaaccg gctgcattcg acacactcag cggccggttg accgcacgcc gagccgaaaa
     7141 cggcatcgag atggacttcc cgctccatcc ggtggtgccg ctcgcggacg acgcggtccc
     7201 tgatgcgctg cgcgagggtc tcggcgtccc gttgcagcac gtcacagccg acagcaaccg
     7261 gtacgtggtc gagcttgccg atgaagcgac gctgcgggag ctgcagccga acctggcgct
     7321 gctgagcaca ctgatgcgcg ccgtcgtcat cacctgtcgc tccgagacgc ccgagttcga
     7381 cttcgtctcg cgcaacttcc tgccgcaccg cggtatcccg gaggatccgg tgaccggcgg
     7441 cgcgcattgc agcctcgcgg actactggcg atcgagactc ggcaaggaga cctttacggc
     7501 ctatcaggca tcccggcgcg gcggtgcgct cggtgtccga atcaccggcg accgggtcat
     7561 cctgaccggc caggccgtta cggtcctgcg gggtacgttg caggcgtaac ggcggcgttg
     7621 cggcacagac gatgggatcg gagaaaggca gaccgtggca accgcaattg acttcagcga
     7681 tccggcgatt caggcaaacc cctacccggt ctaccggcag ttgcgcgagg agtcatcggt
     7741 tgtctggaac gagacgacca gcagctggct catcagccgc tatgacgaca tcgtcgcgct
     7801 gctgaacgac ccgcgcatct cctcggcgcg ggtcgatgcg acctttcgcg tcctgccgga
     7861 ggatgttcag cgggaattgc agccgctgcg cgatgtcctt agccagcgga tgctgctgac
     7921 cgacccgccg acccacacgc ggctcaagaa cctcgtcatg ccggccttcg cggcccgcat
     7981 gagcgagcgg cgccgggcgc gcatccagga gatctgcgac agcttcatcg accggctggc
     8041 cgaggccgac acgttcgacg ttatgcgcga cttcgcgacg ccgctgcccg gctgggtcac
     8101 cggcgatgtg ctgggagtgc cggtcgagcg ccaacagcag ttctcggact ggagccacga
     8161 ccaggtccgc atctacgacc ggctcggcac cgtccacgac cgaatcgccg tcatgcgcca
     8221 ggggcaggcc agtatgctgg cgatgaaggc gtacctcgaa gagatcatcg agcagcgccg
     8281 ccgcctgccg ggcgacgatg tcatcacgat gctggtccag gccgaggagg ctggcgacac
     8341 actcaccaac gacgagatgg tcgtgatggt cgtcgcgatc ctcgtcggcg gcaacaactc
     8401 gaccgcccac ttgatcggca acgcgatcct gacgttgctg cgccagccgg aagcactcgc
     8461 ccgcctgcgc gacgacccgg ctctgatccg cccggccatc gaggaggtca tgcgcttcga
     8521 gagcccggtc cagtccacca gccgcgtcgc gaaggagccg atcgagatcg gcggccagac
     8581 gatcgagccg ggcaacaacg tccacgtcct gctggcctcc ggcaaccgcg acgaggcgca
     8641 gtttccaaca cctgacacct tcgtcatcga ccgcagcccc aaccgccacc tgacgtttgc
     8701 ccacggccca cactactgcc tcggcacctc gctggcccga acgatgaccc agaccgccgt
     8761 ccagaccctc ctcacccgct tcccggacct gcgactcgcc agcgacaccc cggaatggaa
     8821 cgagggcttc tcgttcagga gcctgaagac gttgccggtg gtgagtgcga tgtgacgcgt
     8881 gatgcgtgac gcgtgatgcg tgatgcgatt caggcgtgcg gtcctcacta acgccgttcg
     8941 gctgggtcgc gtcccgcgtc acgaaccccg cgtcccgcgt cacgcgtcac atcgcactca
     9001 ccaccggcag cgtcttcagg ctcctgaacg agaagccatc gttccattcc ggggtgtcgc
     9061 tggcgagtcg caggtccggg aagcgggtga ggagggtctg gacggcggtc tgggtcatcg
     9121 ttcgggccag cgagg
//
LOCUS       DASUGJ010000406         8072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_480503, whole genome shotgun sequence.
ACCESSION   DASUGJ010000406 DASUGJ010000000
VERSION     DASUGJ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480503"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..980)
                     /locus_tag="VFV93_08910"
     CDS             complement(21..980)
                     /locus_tag="VFV93_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX5165501.1"
                     /translation="MRAYRMLTILLLLQSRGQVTARELADRLEVSQRTIYRDLEALSS
                     AGAPVYAERGSGGGWRMLEGAGPDVPGLDEAQVRALLLGGRRDLQEDLGLRGAAELVW
                     ARLEEAIGPRDQPVDDLLLVDSPSWRGRRDEVASLPVIQQAVFRGRRLQMLYARDGEP
                     VQRVVDPLGLVVKGSNWYLVAAVDGQPRTYRVSRVLEATTLPERASRPDGFRLREWWD
                     AQQRDFVERLPRFPVQAMVRGEPLDGLSRVGWYATIEHQSEPDAKGFRVVDVAFQAEH
                     HALGWAFSGGADVIVIEPAELRARLVQYAGVVLRQYGDPALSS"
     gene            1103..1630
                     /locus_tag="VFV93_08915"
     CDS             1103..1630
                     /locus_tag="VFV93_08915"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5165502.1"
                     /translation="MTTRTDTAWDITAGPAIVPEGYTFTDRDQRLLDWSYLTERMEAA
                     KNYWVCTTRPNGRPHAMPTWAVWHDNRLYFDGSPETRRMRNIALNPHVAVHLEDGTNA
                     VIVEGICRPVERPSTEFGIQLAALYAAKYAADGYAPEPDSWNDGGLYAVEPNLALAWT
                     EFGVDMTRWKIRHTP"
     gene            complement(1679..2917)
                     /gene="fabF"
                     /locus_tag="VFV93_08920"
     CDS             complement(1679..2917)
                     /gene="fabF"
                     /locus_tag="VFV93_08920"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5165503.1"
                     /translation="MQRVVVTGMGAVTPIGNDVETFWQNLTAGETGVARITAFDPEPL
                     EVQIGAEVKDFDPTQYADRGDVRRMDRFAQFAVAASKMAIEDAGLEIDPENSEHIGVM
                     MNTGGGGIPTLTREVQNYYTKGPRRVSPFFIPMFAPNMAATQVSITFGIHGPTRGSVA
                     ACAAGSQAWVDSLHLLQRGEAEVVVAGGTEAGMDPVAVAAFQNMRALSRRNDEPERAS
                     RPFDAERDGFVFAEGCGVMILETEEHAKARGARILAEVVGGAMTSDAFHITAPLDDGS
                     GAMKAMKLALKSAGMAPEDIDYLSAHGTSTPLGDKAETVAIKRAFGDHAYRMAVSSIK
                     GAVGHLVGAAGAVAGVAAVKAIETQIAPPTINYEYPDPDCDLDYVPNEARPMKIDATM
                     VNAFGFGGQNAVAIFRRYGT"
     gene            complement(2975..4000)
                     /gene="fabD"
                     /locus_tag="VFV93_08925"
     CDS             complement(2975..4000)
                     /gene="fabD"
                     /locus_tag="VFV93_08925"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5165504.1"
                     /translation="MPTAQLPIESGATGEQVAFVFPGQGSQYVGMGQRLHEISEAARR
                     VFEQADEALGFRLSRLCFDGPADELQDTINAQPAILTVSHAALEALRERWHHAGGAIA
                     PSAVAGHSLGEYSALVSAGSLDFGDAVRLVRERGRLMQEAGRERPGGMAAVIGLSSEA
                     LADVCRMASMDGEIVVVANDNGPGQTVISGELRALERAMELATMRGAKRVVRLGISIA
                     SHSPLMERAGLQLNEAASRFSFSEPSTPVVANLTGRFVTSVEEIRRNVGQQMVRPVQW
                     TGSVYEMVDAGVGTFLEVGPGQVLSGLIRRIKREVKTLSVADFGLPLGSSDSTDNSGS
                     LRLPGRK"
     gene            complement(4134..6125)
                     /gene="ftsH"
                     /locus_tag="VFV93_08930"
     CDS             complement(4134..6125)
                     /gene="ftsH"
                     /locus_tag="VFV93_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5165505.1"
                     /translation="MMDPSGRNPQTPKKPGGGQPQGQPQSRFKIPGWVLWGLLALLAG
                     WYIYMFLWPEDDHRLHINYSSLVTAVESGNVSAVEITGQKVDGDFTQELRWTGTELLL
                     PGQDVPDDVNSDDIETGEKFRVTIPENSQEQLIPILEANDVTIDASEQSQSILPSLLI
                     TVLPLMLFLGLIVFMGRNMSRGQQNVFGFGRSRAKMYDPERPHVTFADVAGEEEAKQE
                     LTEVVDFLKNPMKYHQIGARLPRGILLVGPPGTGKTLLAKAVAGEAGVPFFSVSASEF
                     VEMFVGVGASRVRDLFERAKAASPSIIFVDELDAVGRQRFAGLGGSNDEREQTLNQLL
                     VEMDGFEPNQDVIIVAATNRPDVLDPALLRPGRFDRQVTVGLPDRRGREAILQIHSRG
                     IPLDSSIDLSSIAGGTPGFSGADLANLVNEAALTAARRNRKTVTRADFDEALDKIILG
                     TVRSALLNENDRLVVAYHEAGHAIMAHFSPGSDPLRKVSIVPRGRALGVTIQTPAEDK
                     YNYPKQYLVARLGVMMGGRAAEMIVFNEVTTGAQNDLKEATELAKRMVGLWGMSEEIG
                     PIYLGTGEQHVFLGRELTQDRDMSDATANRADAAVRSFVEAGLTSALALLTERRANLD
                     ALAQRLMEDETLDEDQITELLGAPPVPSEVAAMERTTLD"
     gene            6374..7729
                     /locus_tag="VFV93_08935"
     CDS             6374..7729
                     /locus_tag="VFV93_08935"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165506.1"
                     /translation="MSASPDNQPANAETRLLQEPAFRAWWTARLLSQTAQAALLYSLL
                     ILITNQTDRSIYTSLYVVCSIVPALVFGLFGGWLADRLPQRALLIVLNVIRAVLVVLL
                     LGASAGLVTIFGVTLGIWIVHQFFSPAESATIARLVPPERLDNANSLSNFALTLAQLL
                     GMVLLAPLMLKLPDERLLFAVIAGLYFIAAIYLVRMGRLPAGDAERKRKPALDLRRGW
                     RVVVADRPAFGALIDAVAIGVGMSTLVVIVPYYLVDVLDTSAGNTVFVFAPAVIGLVV
                     GLQLAPVIGRLIGHHRLALLGLLGFSACIALLGLIDPFIDVLRESRIDLHSLEDRLGL
                     SARISATMLLSIPAGFCSAVTNVAARTVLLQRSPEDARGQVLATQATLSNAIALVPTI
                     TVGIAIDLVEVRPVAFVIAIALVTGALLGRRISSGQPVGAPITAPVAGQPTQSNGGRK
                     R"
     gene            7726..>8072
                     /locus_tag="VFV93_08940"
     CDS             7726..>8072
                     /locus_tag="VFV93_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5165507.1"
                     /translation="MNDTGHDDAILRFWTLAQQLKRLRRTGWIDRGVSDPESTAGHSW
                     GVALLAWLLARDERELDRDRVLLLGLVHDLPEAVAGDVTPFDNVRDLTGTIPPEHFST
                     APGYTPAARAAKRA"
BASE COUNT         1344 a         2868 c         2455 g         1405 t
ORIGIN      
        1 gagatccttc ggcgcacgcc tcaggatgac agagcgggat cgccgtattg ccgcaacacc
       61 acgcctgcgt actgaacgag ccgcgcgcga agctcggcag gctcgatgac gatcacatcg
      121 gcgccgccgg agaacgccca gcccagcgcg tgatgctcgg cctggaatgc cacgtcaacc
      181 acgcggaagc ctttcgcgtc cggctccgac tggtgttcga tcgtcgcata ccagccaaca
      241 cgactgaggc cgtcgagcgg ctcgcctcgc accatcgcct gaaccggaaa acgaggcaat
      301 cgctcgacga agtcgcgctg ctgcgcgtcc caccactcgc gcaagcggaa tccatccggc
      361 cggctggcgc gctccggcag ggtcgtcgcc tccagcaccc gcgacacgcg ataggttcgc
      421 ggttgcccat cgacggctgc caccaggtac cagttgctgc ccttcacaac cagcccaagc
      481 ggatcgacaa cccgctgtac cggctcgccg tcccgagcgt agagcatctg caaccgccgg
      541 ccacgaaaga cggcctgctg gatgacgggt agcgacgcga cctcgtcgcg tcgcccacgc
      601 caggatggac tgtccaccag cagcaggtcg tcgaccggct gatcgcgcgg tccgatcgcc
      661 tcttcgagtc gcgcccagac cagctcggca gcaccgcgca acccgaggtc ctcctgcaag
      721 tcgcgccggc ctcccagcag cagcgcccga acctgcgcct catcaagccc cggaacatcc
      781 ggcccggcgc cctccagcat ccgccagcca ccaccggaac cccgctcggc gtagaccggc
      841 gcaccggccg acgacaacgc ctccagatca cgatagatcg tgcgctgtga gacttccagc
      901 cgatcggcca gctcgcgcgc tgtcacctgc ccacgcgact ggagcagcag gaggatcgtc
      961 agcattcgat acgcacgcat cacacctccg tcgtgatctc gaaatatgac agaggatgac
     1021 acaggacgcc ggcatactgg taatgacacg gttggcaccc gtgtcagcga cggttgaccg
     1081 actgactcga cgtggaggca gcatgacgac acgcacggac acagcatggg acatcacggc
     1141 cggaccggcg atcgtgccgg agggctacac gttcacggac cgtgaccaac ggctgcttga
     1201 ctggtcgtac ctgacagaga ggatggaggc ggcgaagaac tactgggttt gcacaacccg
     1261 ccccaacggc cggccgcacg cgatgccgac ctgggcggtc tggcacgaca accgcctcta
     1321 cttcgacggc agccccgaga cacgacgcat gcgcaacatc gctctcaacc cacacgtagc
     1381 cgttcacctc gaagacggca ccaacgccgt catcgtcgaa gggatctgca gacctgtcga
     1441 gcgaccgtca acggagttcg gtatacaact ggcagccctc tacgccgcca agtacgccgc
     1501 cgacggctac gcaccagaac cagatagctg gaacgacggc ggactctacg cagtcgagcc
     1561 gaatctcgcg ttggcgtgga ccgaattcgg cgtcgacatg acgcgctgga agattcgcca
     1621 cacgccataa cgcatctcga atcacaatgg tccatccggt acgggcggtt gtgattgatc
     1681 aggtgccgta gcgccggaag atcgcgactg cgttctggcc gccgaagccg aaggcattga
     1741 ccattgtcgc gtcgatcttc atcggccgcg cctcgttggg gacgtagtcc aggtcgcagt
     1801 cggggtcggg gtactcgtag ttgatcgttg gtggcgcgat ctgcgtctca atcgccttga
     1861 cggcagcgac gccggccacc gcgccggccg cgccaaccag gtgtccgacg gcgcccttga
     1921 tcgatgagac ggccatccgg taagcatggt cgccaaacgc gcgcttgatc gcgaccgtct
     1981 cggccttgtc gcccagcggc gtgctggtgc cgtgcgcgga gaggtagtcg atgtcttccg
     2041 gcgccatccc ggccgacttc agcgccagct tcatcgcctt catcgcgccg gagccatcgt
     2101 cgagcggcgc cgtgatgtga aaggcgtccg acgtcatcgc gccgccgacg acctcggcca
     2161 gaatccgcgc cccacgcgcc ttggcgtgct cttcggtttc gaggatcatc accccacagc
     2221 cctcggcgaa gacgaacccg tcccgctcgg catcgaacgg ccggctggcc cgctccggct
     2281 cgtcgttgcg tcgtgacagc gcgcgcatgt tctggaacgc agcgaccgcg accggatcca
     2341 tccctgcttc ggtcccaccg gcgaccacga cctccgcctc accacgctgc agcagatgga
     2401 gcgagtcaac ccacgcctgc gagccggccg cgcaggccgc caccgagccg cgcgtcgggc
     2461 cgtgaatgcc gaatgtgatc gacacctggg tcgcggccat gttcggggcg aacatcggga
     2521 tgaagaatgg actgacgcgc cgtggtcctt ttgtgtagta gttctggact tccctggtca
     2581 gcgtcggaat cccgccgccg ccggtgttca tcatgacgcc gatatgttcc gagttctcag
     2641 ggtcgatctc gagtccagcg tcctcgatcg ccatcttcga cgccgcgacc gcgaactggg
     2701 cgaagcgatc cattcgccgt acatcgccgc ggtcggcata ctgcgtcgga tcgaaatcct
     2761 tgacctcagc gccgatctgg acttcgaggg gctccggatc gaaggcggtg atccgcgcga
     2821 cgcccgtctc gcctgccgtc aggttctgcc agaacgtttc gacatcgttc ccgattggcg
     2881 taaccgcgcc catacctgta acgacgaccc gttgcacgct tgcctgttcc tccccgaccg
     2941 tcacatcgcc cggcgtcagg ccgcgcgccg gcgtctattt cctgcccggc agcctcaaac
     3001 tgccactgtt gtccgtcgaa tccgatgagc caagcggaag cccgaaatcc gcgaccgaga
     3061 gcgtcttgac ctcgcgcttg atgcgccgga tcaggccgct cagcacctgt ccggggccga
     3121 cctcaaggaa ggtcccgaca ccggcgtcga ccatttcgta gaccgacccg gtccactgga
     3181 ctggccggac catctgctgg ccaacgttgc ggcgaatctc ctcgaccgat gtcacgaagc
     3241 ggccggtcag attcgcgacc accggcgttg acggctcgct gaagctgaac cggctggccg
     3301 cctcgttgag ctgcaatccg gcacgctcca tcagcggcga gtgggacgcg atgctgatcc
     3361 cgagccggac gacgcgcttc gcgccgcgca tggtcgccag ttccatcgcc cgctccagcg
     3421 cgcgtagctc gcccgagatc acggtctggc cgggaccgtt gtcattggcg acgacgacga
     3481 tctcaccatc catcgaggcc atccggcaga catcagccag cgcctcgctg ctgagcccga
     3541 tcacggccgc catcccgccg ggccgttccc ggccggcctc ctgcatcaga cgcccacgct
     3601 cgcgcaccag ccggaccgcg tcgccgaagt ccaacgaccc cgccgagacc agcgcgctgt
     3661 attcccctag cgagtgtcca gcgaccgcgc tgggcgcgat tgcgccgccg gcgtggtgcc
     3721 agcgttcgcg cagcgcctcg agtgccgcgt ggctgaccgt caggatggct ggttgggcgt
     3781 tgatagtgtc ctgcaactcg tcggcaggtc cgtcaaagca gagccgtgat aaccggaaac
     3841 cgagcgcctc gtccgcctgc tcaaagaccc gtctggcggc ctcggaaatc tcgtgcagac
     3901 gctggcccat cccaacgtac tggctcccct ggcccgggaa gacaaacgcg acctgctcgc
     3961 cagtggctcc agactcgatc ggcaactgtg cggttggcac ctggcccccc aatgacatca
     4021 caagctctga cacaacgcga tgcgaaaaga gacatcacca catgtcgcaa tataccagaa
     4081 ttcacgaatt aggcccctcc ggcggcgttc agaggggcca cccatgcgtc ctgctaatcg
     4141 agcgtcgtcc gctccatcgc cgccacttca gatggcaccg gcggcgctcc gagcagttcg
     4201 gtgatctggt cctcgtccag cgtctcgtcc tccatcaacc gctgggcaag tgcgtcgagg
     4261 ttcgcccgtc gttcagtcag cagtgcgagc gccgacgtca gccccgcttc gacgaacgag
     4321 cggactgccg cgtcagcccg gttggccgtc gcgtcgctca tgtcgcgatc ctgcgtcaac
     4381 tcacgcccga ggaagacatg ttgctcgccg gttccgaggt agatcgggcc gatctcctcg
     4441 ctcatacccc acaggccgac catccgcttg gccagctcgg tcgcttcctt gaggtcgttc
     4501 tgggcgccgg tcgtcacttc gttgaagacg atcatctcgg ccgctcggcc gcccatcatc
     4561 acgcccaacc gggccaccag atactgcttc gggtagttgt atttatcctc ggccggcgtc
     4621 tggatcgtca cgcccagcgc ccgcccgcgc ggcacgatcg acaccttccg cagcgggtcg
     4681 gaacccggcg agaagtgcgc catgatcgcg tgccccgctt cgtggtaggc cacgaccagc
     4741 cggtcgttct cgttcagcag ggccgagcgg accgtcccga ggatgatctt gtccagcgcc
     4801 tcgtcgaagt cggcccgggt caccgtcttg cggttgcgcc gcgccgccgt cagcgccgcc
     4861 tcgttgacga ggttcgccag gtcggcgccg gagaagcctg gtgtcccgcc ggcgatgctg
     4921 ctgaggtcga tgctgctgtc gagcgggatc ccccgcgagt gaatctgaag gatcgcttcc
     4981 ctcccgcgcc ggtccggcag cccgaccgtg acctgccggt cgaatcggcc agggcgcaac
     5041 agggccgggt cgaggacatc gggccggttg gtcgccgcga cgatgatgac atcctggttc
     5101 ggctcgaagc cgtccatctc gaccagcaac tggttcaggg tctgctcgcg ctcgtcattg
     5161 ctgccgccga gcccagcgaa gcgctgccgg ccgaccgcgt cgagctcgtc gacgaagatg
     5221 atcgacgggg atgcggcttt cgcccgctcg aacaggtcac ggacacggct ggcgccaacg
     5281 ccaacgaaca tctcgacgaa ctcggacgcg ctgacgctga agaacggcac gcccgcctcg
     5341 ccggcgacgg ccttcgccag cagcgtctta ccggtgcccg gaggaccgac cagcaggatg
     5401 ccgcgcggca gccgcgcgcc gatctggtgg tacttcatcg ggttcttgag gaaatcgacg
     5461 acctcggtca gttcctgttt ggcctcttcc tcgccggcga cgtccgcgaa cgtcacatgt
     5521 ggccgctcgg ggtcgtacat tttggcccgc gagcggccga agccgaacac gttctgctgc
     5581 ccgcgcgaca tgttgcggcc catgaagacg atcagcccca ggaacagcat tagcggcagg
     5641 acggtgatca gcagactcgg caggatcgac tgcgattgct cgctcgcatc gatcgtgacg
     5701 tcgttcgcct ccaggatcgg gatcaactgc tcctgcgagt tctcgggaat cgtgacccgg
     5761 aacttctcgc cggtttcgat atcgtcgctg ttgacgtcat ccggcacgtc ctggcccggc
     5821 aggagcaact ccgtgccggt ccagcgcagt tcctgcgtga aatccccgtc caccttctgg
     5881 ccggtgatct cgaccgctga aacgttgccg gattcgacgg cagtgaccag cgaggaatag
     5941 ttgatgtgca gccggtgatc gtcctccggc cagaggaaca tatagatgta ccagccggcc
     6001 agcagcgcga gcagccccca gagcacccag ccgggaatct tgaagcgcga ctgcggctgg
     6061 ccctgtggct ggccgcctcc gggcttcttg ggtgtctgcg ggtttcgacc cgaggggtcc
     6121 atcatcgttc cgtcgactcc tctaccatga tagccggcat cttcgctgag acatgtgttg
     6181 acccatgccg gccattgcgc gcatcgtcgc gcgttcaggt ccgctctaag cataatctac
     6241 cgatcgccca ttcggacaca cccaaccatg gccgagtcgg ccgtgtgccg gccggcgtcc
     6301 atcacaatta gtggcacgga cgctgccaac gtcaatccag cagcgccgaa cgagacctcg
     6361 attgtggagc ctgatgtctg caagtcctga caaccagcct gccaatgccg agacccgcct
     6421 gctgcaggag ccggcgtttc gcgcctggtg gacagcccgt ctgctctcgc agaccgccca
     6481 ggcagcgctc ctctacagcc tgctgatcct catcaccaat cagaccgacc gcagcatcta
     6541 cacctcgctc tacgtcgtct gctcgatcgt tccggcgctc gtcttcggtc tgttcggagg
     6601 ctggctggcc gaccggctcc cgcagcgtgc gctgctgatc gtcctgaacg tcatccgggc
     6661 cgtgctggtc gtgctcctgc tcggcgcgtc agccggcctg gtgacgatct ttggcgtcac
     6721 gcttggtatc tggatcgtcc atcagttctt ctcgccggcc gagtcggcga cgatcgcccg
     6781 actcgtccct cccgagcggc tcgacaacgc caactccctc tctaacttcg cgctgacgct
     6841 ggcccagctg ctcggcatgg tcctgctggc gccgttgatg ctcaagcttc ctgatgaacg
     6901 gctgctcttc gcagtgattg ccgggctgta cttcatcgct gccatctacc tcgttcgcat
     6961 ggggcgcctt ccggccggcg acgccgagcg caagcgcaag ccggctctcg atctgcgtcg
     7021 cggctggcgc gtggtcgtcg ccgaccgtcc ggcatttggg gcgctcatcg acgccgtcgc
     7081 gattggcgtc ggcatgagca cactggtcgt catcgttccc tactacctgg tcgatgtgct
     7141 tgacaccagc gccggcaaca ctgtcttcgt cttcgcgcct gccgtcatcg ggctggtcgt
     7201 tgggctgcag cttgcgccgg tcatcggccg gctgatcggt catcaccggc tggcgttgct
     7261 cgggctgctc ggcttctccg cctgcatcgc gctactcggc ctgatcgacc cgttcatcga
     7321 cgttctccgc gaatcgagga tcgatctgca cagtcttgaa gaccggctcg gcctctcagc
     7381 ccggatctca gcgacgatgc tgctgtcgat cccggccggc ttctgctcag ccgtcacgaa
     7441 cgtggccgcc cgaaccgtcc tgcttcagcg ttcgccggaa gatgcgcgcg gtcaggtgct
     7501 ggcgacccag gcgaccctct cgaacgcgat cgccctcgtc ccgacgatca cagtcggcat
     7561 cgcgatcgac ctggtcgagg tgcgcccggt cgccttcgtg atcgccattg cgctcgtcac
     7621 cggcgcgctg ctgggtcgta gaatcagctc cggccagcct gttggagcac cgatcacggc
     7681 tccggtggcg ggacaaccga cgcaatcaaa tggaggacgg aagcggtgaa cgacaccggc
     7741 cacgacgacg cgattctgcg gttctggaca ctggcgcagc agctcaagcg cctgcgccgg
     7801 accggctgga tcgaccgtgg cgtcagcgat ccggagtcga ccgccggtca ctcctggggc
     7861 gttgccctgc tggcctggtt actggcccgc gatgagcggg aactcgaccg cgaccgcgtc
     7921 ctgctgctcg gcctcgtcca cgatctgcct gaagccgtcg ccggagatgt cacgccgttc
     7981 gacaacgtcc gtgacctgac cggcacgatc ccgcccgagc atttcagcac cgcgcccggc
     8041 tacacgccgg ctgcccgagc cgccaagcgg gc
//
LOCUS       DASUGJ010000407         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_480543, whole genome shotgun sequence.
ACCESSION   DASUGJ010000407 DASUGJ010000000
VERSION     DASUGJ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480543"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1654)
                     /locus_tag="VFV93_08945"
     CDS             complement(59..1654)
                     /locus_tag="VFV93_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165508.1"
                     /translation="MNAIHSDPATPYPKRWWALVVLCLSLLVISLDNTILNVALPTLV
                     TDLSASSSQLQWVVDAYVLVFAGLLLTAGALSDRYGRRLGLFSGFAIFAVGSAFAAWS
                     SSPEQLIAWRAVMGVGGALIMPSTLSILTNTFPANERPKAIAIWTMTAGIGIPTGPVI
                     GGWLLEHFWWGSVFLINIPIIVAALLAGIVLLPESKDPNAKPLDPGGALLSIAGLGTL
                     IYAIIEAPNHGWTSASTLLAFGVAAVILAAFVLWEHRVDQPMLDMGLFRNMRFTGASI
                     SVTLVFFAMLGMLFSLTQYLQFVLGYDPLEAGIRITPVALGIIVGAGSSTRLASRLGS
                     KIVVTWGLTVVAGGLGLLSTIDTDSGYGLVAAALIVLGYGMGTAMAPATEAIMGAVPK
                     DNAGVGSAVNDATRQVGGALGVAILGSLLSTGYTSSMEGVTAHLPAPAASAANDSVGA
                     AVGIAARLGGPEGAALASAARAAFTDGMSTSLLVAAGFALAGAIFAAVFLPAHAAAAE
                     ESGPMRDTEREEIEFVALETTGR"
     gene            2066..>2580
                     /locus_tag="VFV93_08950"
     CDS             2066..>2580
                     /locus_tag="VFV93_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX5165509.1"
                     /translation="MATGVNGRHGFSTRAIHAGEGSDPTTGAHNTPIYQTSTYAFHSL
                     EQKAAVMSGEAEGYVYTRGGNPTTSAFEQKVAALEGAEAATAGGSGMAVIMAAMRAFV
                     RSGDHIVASDDVYHWATIYLEEEAQLDRIDVTRVDIGDLDAVRAALRPETKVIYTEFL
                     SNPQIKVADIAG"
BASE COUNT          480 a          809 c          917 g          374 t
ORIGIN      
        1 gcgctgcggg ggcgtccggg ccggcgttgt gtcgtgtcgg tcgtgtcagt ctgagcggtc
       61 aacggcctgt cgtctccagc gcaacgaact cgatctcttc ccgctcagta tcgcgcatcg
      121 ggccggactc ttccgcagcg gcggcgtggg ccggcaggaa gacggcggcg aagatggcgc
      181 cggccagcgc gaagccggct gcaacgagca gcgatgtgct cataccgtcg gtgaaggcgg
      241 cgcgagctgc cgaggccagc gcagcaccct ccggcccgcc cagccgggca gcgatcccaa
      301 cggcggcgcc gaccgagtcg ttggcggccg atgcagccgg ggccggcaga tgagcggtga
      361 caccctccat tgaggacgtg tagccggttg agagcaggct gccgagaatg gcgacaccga
      421 gcgctccgcc gacctgacgg gtggcgtcgt tgacggccga gccgacgccg gcgttgtcct
      481 tcgggacggc gcccatgatc gcctcagtgg ccggggccat cgccgttccc atcccgtagc
      541 cgaggacgat cagcgcggcg gcaaccagac catagccgct atcggtatcg atcgtcgaga
      601 gcagcccgag gccgccggcg acgaccgtca ggccccaggt gacgacgatc ttcgagccga
      661 gccggctcgc cagccgggtg ctgctaccgg cgccaacgat gatgccgagc gcgaccggcg
      721 tgatgcggat accggcctca agcggatcgt agccgaggac gaattgcagg tactgcgtca
      781 gcgagaagag catccccagc atcgcgaaga agaccagcgt gaccgagatg ctggcgccgg
      841 tgaaacgcat gttgcggaac aggcccatgt ccagcatcgg ctggtccacc cggtgctccc
      901 agaggacgaa ggcggccagg atgacggccg cgacgccgaa ggcaagcagc gtcgaagcgc
      961 ttgtccagcc gtggttgggc gcctcgatga tggcgtagat caatgtcccc aggccggcga
     1021 tcgagagcag cgcgccgccg ggatcgagcg gcttggcgtt cgggtccttc gactccggca
     1081 ggaggacgat cccggccagc agcgcggcga cgatgatcgg gatgttgatc aggaagaccg
     1141 atccccacca gaagtgctcc agcagccagc cgccgatgac tggtccggtc gggatgccga
     1201 tcccggctgt catcgtccag atcgcgatcg cctttgggcg ctcgttggcc gggaaggtgt
     1261 tggtcaggat cgagagggtg ctgggcatga tcagcgcgcc gccaacaccc atgacggccc
     1321 gccaggcgat cagctgctcc ggcgagctgg accaggcggc gaaggccgag ccaacggcga
     1381 agatggcgaa accgctgaac aggccgaggc ggcggccata gcggtcggag agggcgccgg
     1441 ccgtcagcag cagcccggcg aagacgagca cgtaggcatc aaccacccac tgcaactggc
     1501 tgctgctggc cgagaggtcg gtcaccagcg tcggcaacgc gacgttgagg atcgtgttgt
     1561 cgaggctgat caccagcaag ctcaggcaga ggacgaccag cgcccaccag cgcttcggat
     1621 acggggtagc ggggtccgag tgtatcgcgt tcattggtag ctcctgcttc ggcagcataa
     1681 cggtactccg tcgtatcgtt attggcagag tagcaccaat tgatacggga cgcaagagtc
     1741 tcgtaatacg attcagccga acggggctga tgaggaaccg cacccgttgc cctgtcatcc
     1801 tgagcgcaag cgaaggatcg cttaaccgta cgggcgtgcg agcgcaaatc ggcagatggc
     1861 cgttgacccg accgaactac tcacgccccc agcgccaggc gatccttcgc tgtggctcag
     1921 gatgacaggg caaggggccg gctcctcatt ggcgccgttc gcttggcgcg ttgacggtgg
     1981 aggagcatcg cggcgggctg ccggtcgttc gtggtatgca acaatcggga cattgggtag
     2041 cagtgacaac gaggagggga acgggatggc aacgggggtc aacggcaggc acgggttctc
     2101 gacgcgggcg attcatgccg gggagggcag cgacccgacg accggggcgc acaacacgcc
     2161 gatctatcag acatcgacct acgccttcca ctcgctggag cagaaggcag ccgtcatgag
     2221 cggcgaggcc gaggggtacg tctacacgcg tggcggcaac ccgacgacca gtgccttcga
     2281 gcagaaggta gcggcgctgg agggggccga ggcagctacg gccggcggct cggggatggc
     2341 cgtcatcatg gcggcgatgc gcgcgttcgt ccgttccggc gaccatatcg tcgcttccga
     2401 cgacgtctac cactgggcga cgatctacct tgaagaggaa gcccagcttg accggatcga
     2461 cgtgacgcgg gtcgatatcg gcgacctgga cgccgtccgg gcggcgctgc ggccggagac
     2521 gaaggtgatc tacaccgagt tcctctccaa cccgcagatc aaggtcgccg acatcgccgg
//
LOCUS       DASUGJ010000408         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_62292, whole genome shotgun sequence.
ACCESSION   DASUGJ010000408 DASUGJ010000000
VERSION     DASUGJ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62292"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFV93_08955"
     CDS             <1..376
                     /locus_tag="VFV93_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165510.1"
                     /translation="LAGHDVQVLVAIPAEYVHLVDGPIDVVFKTPSGMNRSVAFTDDG
                     FNGFGETVSFVDNPRSPVNVKGAFTLDVRVTVPIDESQATSDLKTDKIPTWITVTEGN
                     TSVVLHGWSDGSWVTARIGNVR"
     gene            407..892
                     /locus_tag="VFV93_08960"
     CDS             407..892
                     /locus_tag="VFV93_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165511.1"
                     /translation="MNVRRLVTAGSLGLLALMLLVMPAAAGVSWCRADPIVELNGTPI
                     QVWVAVSAEHEHLVSGPIQVMVFTSDEVDTNTTYLDDGFNGYGEEVTFRRRNWKHNDE
                     SFPVEIKVKVPLDNRQVRREFGMRDIPILIEIITGDGQTISIEGRSSGASASMEVHGS
                     H"
     gene            1039..2433
                     /locus_tag="VFV93_08965"
     CDS             1039..2433
                     /locus_tag="VFV93_08965"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX5165512.1"
                     /translation="MLRESLARASHRFGYADLPTGLLRYVLFITAAGVLAPFAVIALA
                     WQPLTGRELLQAGMLLVMATISERFPLHLTHKTNINITSAAVIVMILLLPVWVPGLLV
                     LIGAGVAQSLRRAEVMEAGFNVGQTVLYVTAGAICYGLFASVELGPRIGDFGSLAVIA
                     VVATVMHLLNTGMVAVAGGLQLGLNPLRVWASTIALDVMPHVTLTGLGTIAAMLAVDQ
                     PLALPFLALPAVLVHQSVRQTVQLRVDTHEALASLVEVMELRDPYTAGHSRRVAALSR
                     TLATRLGLTEEEADVVEHAGRVHDIGKAGIDQVILTKPGQLSDVEWNQMRNHPDLGAN
                     VVSRFAAYRHGAKIVRHHHESWDGSGYPDGLTGEQIPLGSRIIAVADTFDALTSSRPY
                     RDAMSIEDAISVLEAGAGLQWDERVVSTMVSYLRDLHGELPTFRPAGGPPVPVQPFAD
                     PATGRGPAIPTPVD"
     gene            2452..>3403
                     /locus_tag="VFV93_08970"
     CDS             2452..>3403
                     /locus_tag="VFV93_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165513.1"
                     /translation="MPPLKTVTRIYITLLTLTALMALGVGLNDASGVDVQHAVLSVTF
                     VAAFALSVMFPFQFAWKTRQHLDTTVILAAVLIYEPGIAMAIVATGTIVAQLVRRESW
                     AQFLFNSAQMVLQAALAGAILAEFHWTPTSPSFNSIETLLAIFLAGVALYLINTLTVA
                     FIIGLEDGTSPLKVWLRSTAILDRTETLLHVGQIGIALLAAIVANAHLWALALLLLPV
                     AAMYASLHHHVSVRRRVEEALRGTEANLREAQRLAHLGSWEWNLATQERLWSDEFDRI
                     LGTEPSDADLETDIFSGVVHPDDSEMVQGTLGRALRGETPA"
BASE COUNT          559 a         1113 c         1103 g          628 t
ORIGIN      
        1 cctcgctgga catgatgtcc aggtgctggt tgcgatcccc gccgaatatg tccacctcgt
       61 cgacggacca attgatgtcg tcttcaagac gccgtccggc atgaaccggt cggtcgcctt
      121 cactgatgac gggtttaacg gctttggcga gaccgtctcc tttgttgaca acccacgcag
      181 cccggtcaac gtgaagggcg ccttcacgct cgatgtccgg gtgacggttc cgattgacga
      241 gtcgcaggcg acttcagatt tgaagactga caagatccca acctggatca ccgtgacaga
      301 gggaaacacg tcagtcgtgc tccacggctg gagcgatgga tcgtgggtaa cggcccgcat
      361 cggcaacgtc cgctaggcgg tcaggatggg aagaggcatt cagacgatga acgttcgcag
      421 attggtgaca gctggtagcc tcgggttact ggcgctgatg ctgctggtca tgccggcggc
      481 tgccggcgtg agctggtgcc gggccgatcc gattgtggaa ctgaacggca cgccgatcca
      541 ggtctgggtc gcggtcagcg ccgagcacga acacctcgtc tccggcccga tccaggtcat
      601 ggtcttcacg tcggatgagg tcgacacgaa caccacctac ctcgacgacg gcttcaacgg
      661 ctatggcgaa gaagtgacgt tcaggcgccg caactggaag cacaacgacg aaagctttcc
      721 ggtcgagatc aaggtcaagg ttccgctgga taaccggcag gtgcgccgcg agttcggcat
      781 gcgcgatatt ccgattctga tcgagatcat caccggcgac ggtcagacga tcagcatcga
      841 gggccgaagc tccggcgcga gcgcctcgat ggaagtacac ggatcgcatt gacggggcca
      901 accggccagg ctgggttagt acggatcggc cacgccggcc tcatcagccg gcgtggtatc
      961 gtcaattgag gtaacacaat ggtgctggat gggcgtgttc tgcccacagt caatggcccg
     1021 aaaggcagcc cggcagcagt gctcagggaa tcgctcgcgc gcgcttcgca ccggttcggc
     1081 tacgctgatc tgccgaccgg tcttctgcgc tatgtgctgt tcatcacggc tgccggcgtg
     1141 ctcgcgccgt tcgccgtgat cgcgctcgcc tggcaaccgc tcacgggccg cgaactgttg
     1201 caggccggca tgttgctggt catggcgacc atctcggaac gcttcccgct ccacctcacc
     1261 cacaagacga atatcaatat cacctcggcg gccgtcatcg tcatgatcct gctgctgccg
     1321 gtctgggtcc cgggtctgct cgtcctgatc ggagccggcg tcgcccagag cctgcggcgg
     1381 gcagaggtca tggaggccgg cttcaacgtc gggcagacgg tgctctacgt cacggccggc
     1441 gcgatctgct acggcctgtt cgcgtcagtc gagctggggc cgcgtatcgg cgattttggc
     1501 agcctggcgg tgatcgccgt cgttgcgacc gtcatgcatc tgctcaacac cggcatggtc
     1561 gccgtcgccg gtgggctcca gctcggcctc aacccgctcc gggtctgggc ctcgacgatc
     1621 gcgctggatg tgatgccgca tgtgacattg acggggctcg gcacgatcgc cgctatgctg
     1681 gccgtcgacc agccgctggc actgccgttt ctggcactac cggccgttct cgtccaccag
     1741 tcggtgcgcc agaccgtcca gttgcgcgtc gatacgcatg aggcgctggc gtcgctggtc
     1801 gaggtcatgg agctgcgcga tccgtatacg gcgggacact cgcggcgggt tgccgcgttg
     1861 tcgcgtacgc tggcgacccg gctcggcctg accgaggaag aggccgatgt cgtcgagcac
     1921 gccgggcgcg tccatgatat cggcaaggcc ggcatcgacc aggtcatcct gaccaagccc
     1981 ggccaactgt ccgatgtcga gtggaaccag atgcgcaacc atccggacct cggcgcgaac
     2041 gtcgtctccc gcttcgcggc ctatcgccac ggcgcgaaga tcgtccgcca tcaccacgaa
     2101 tcatgggacg gctccggcta cccggacggg ctgaccggcg agcagattcc actcggttca
     2161 cgcatcatcg ccgtcgccga cacgttcgac gcgttgacct ccagccggcc gtatcgggac
     2221 gccatgagta tcgaggatgc gatcagcgtc ctcgaagccg gcgccggcct gcaatgggac
     2281 gagcgcgtcg tctcgacgat ggtcagctac ctgcgcgacc tgcatggcga attgcccacg
     2341 ttccggccag ccggtggtcc gccggtccca gttcagccgt tcgcagaccc ggccaccggc
     2401 cgtggtcctg ccattccgac acctgtcgat tagcggagga ggggcgacgc gatgccaccg
     2461 ctgaagacag tgacgcggat ctacattacg ttgctgaccc tgaccgcgtt gatggcgctg
     2521 ggcgttggcc tgaacgatgc ctccggcgtc gatgtccagc acgcggtgct gtcggtcacc
     2581 ttcgtggccg cgttcgcgct gtcggtgatg ttcccgttcc agtttgcctg gaagacgcgc
     2641 cagcatctcg acacgaccgt catcctggcc gccgtcctga tctacgagcc gggcatcgcg
     2701 atggcgatcg ttgccaccgg cacgatcgtt gcccagctcg tccggcgcga atcgtgggcg
     2761 cagttcctgt tcaacagcgc ccagatggtc ctgcaagccg cgctggccgg cgcgatcctc
     2821 gctgagttcc actggacccc gacctcgccc agcttcaact cgatcgagac gctgctggcg
     2881 atcttcctgg ccggcgtggc cctctacctg atcaatacgt tgacggttgc cttcatcatc
     2941 ggcctggagg acgggacctc gccgctcaag gtctggctgc gctcgacggc gattctcgat
     3001 cgcacggaga cgctgctgca tgtcggccag atcggcatcg cgctgctggc ggcgatcgtc
     3061 gcgaacgcgc acctctgggc gctggcgctg ctgctgctgc cggtcgccgc gatgtatgcc
     3121 tcgctgcatc accacgttag cgtccggcgg cgcgttgaag aggcgttgcg tggaaccgag
     3181 gccaacctgc gcgaggcgca gcggctggcg catctcggca gctgggagtg gaacctcgcc
     3241 acccaggaac gtctctggtc ggacgagttc gaccgcatcc tcgggaccga gccgagcgat
     3301 gccgatctgg agacggacat cttcagcggc gtcgtccacc ccgacgacag cgagatggtc
     3361 cagggcacgc tcggccgggc gctgcgtggc gagacaccgg cca
//
LOCUS       DASUGJ010000409         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_618046, whole genome shotgun sequence.
ACCESSION   DASUGJ010000409 DASUGJ010000000
VERSION     DASUGJ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618046"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFV93_08975"
     CDS             complement(<1..552)
                     /locus_tag="VFV93_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5165514.1"
                     /translation="MVTMDMQSEERVRQWDRAHVFHSWSAQGKIDPMPVVGGAGCEFW
                     DMEGKRYLDFSSQLIYSNLGHQHPTVVNAIKEQAERLCVIQPSFANDKTAELAHLLAE
                     RSPGDLNKVFFTNGGAEANENAIKLARFHTGRHKLLAAYRSYHGATHGAIALTGDPRR
                     WASEPAISGVVHFMGPYTYRSSFH"
     gene            complement(624..1976)
                     /locus_tag="VFV93_08980"
     CDS             complement(624..1976)
                     /locus_tag="VFV93_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5165515.1"
                     /translation="MTITTNVVTLDELVQQQMEYWQIPGVVIGVLKEGEIRLESWGIA
                     NINGVEPMRPEMLVRVASISKVFTATLAMILAEDGVVSLDTPVAEYLPELRLADENVQ
                     RTITLRHLLSHGSGLHGDFFIDMGQGEDALAKAIAEFHTLRQVYQPDELWAYTNSGFH
                     LAGRVLEVRGGKPFDVLMRERIFEPLGMERSCFFAHEILCWPHAVGHAPVAPGSREHR
                     VAGQYYPRNRFPAGGIASNALDLLRFAAFHMGDGGDILSAGSLAAMREPQRKAGNWAH
                     EWGIGWDLRRIDGVQVSSHGGSINGFKSQLTVIPERQTALVMLTNSDYGSNANLRFDR
                     FLLEQECGLVEQQPKPVSVPAGTLERYAGRYTHPDADITLTVEGDRLRIVATGGWPGI
                     EGRVDYPPDYAAPISDTAFLIVEGDSTGTVCDMILHSDGTPRFLRRGGRLHDRVTRDA
                     "
     gene            2162..>2357
                     /locus_tag="VFV93_08985"
     CDS             2162..>2357
                     /locus_tag="VFV93_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165516.1"
                     /translation="MRRDARQSVDLATVDPAEPAPAGVRRRGPFRVSPRVLASPAYRR
                     IWVSGIIYYSARWVEIITTGW"
BASE COUNT          380 a          833 c          744 g          400 t
ORIGIN      
        1 gtggaacgac gagcggtagg tgtacggccc catgaagtgg acgacgccgg agatggccgg
       61 ctccgacgcc cagcggcgcg ggtcgccggt cagcgcgatc gccccgtggg tcgcgccgtg
      121 gtaggagcgg taggcggcca gcagcttgtg gcggccggtg tggaagcgcg ccagcttgat
      181 cgcgttctcg ttcgcctcgg ccccgccgtt ggtgaagaag accttgttca ggtcgcccgg
      241 cgagcgctcg gccagcaggt gcgccaactc ggccgtcttg tcattggcga acgacggctg
      301 gatcacacac agccgctcgg cctgctcctt gatcgcgttg acgacggtcg ggtgctggtg
      361 ccccaggttg gagtagatca gctgcgagga gaagtcgaga taacgcttcc cctccatgtc
      421 ccagaactcg cagcccgccc cacccacaac cggcatcggg tcgatcttcc cctgcgccga
      481 ccatgaatga aacacatgcg cccggtccca ctgccggacc cgttcttcgc tctgcatgtc
      541 cattgtgacc atgaggatgt cctttcgtga cgcgtgacgc gtgatgcgtg acgcgaaagt
      601 ccgaaccaaa tccgcgtgac gcgtcacgca tcacgcgtca cccgatcgtg caaccgccca
      661 ccccgccgca ggaaccgtgg ggtgccgtcg ctgtgcaaga tcatgtcgca gacggtgcca
      721 gtgctgtcgc cctcgacgat caggaaggcg gtgtcgctga tcggggcggc gtagtcgggc
      781 gggtaatcaa cccgaccctc gatccccggc cagccgccgg tcgcgacgat ccgcagcctg
      841 tccccttcga ccgtcagcgt gatatcggcg tcggggtgcg tgtagcggcc ggcgtagcgt
      901 tccagcgttc cagctggcac cgagaccggc ttcggctgct gctcgacgag cccgcactcc
      961 tgctccagca ggaagcggtc gaagcgcagg ttggcgttgc tgccgtagtc gctgttggtc
     1021 agcatcacca gcgccgtctg ccgctccggg atgaccgtca gctgcgactt gaagccgttg
     1081 atcgagccgc cgtgcgagct gacctgaacg ccgtcgatcc ggcgcaggtc ccagccgatg
     1141 ccccactcgt gcgcccagtt gccggccttg cgctgcggct cgcgcatggc agccagcgaa
     1201 ccggcgctga gaatatcccc gccgtcgccc atatggaacg ccgcgaagcg cagcaggtcc
     1261 aacgcgttcg acgcgatccc accggccggg aagcggttgc gcgggtagta ttgccccgcc
     1321 acccggtgct cgcggctgcc cggcgcgacc ggcgcatgac cgaccgcgtg cggccagcag
     1381 aggatctcgt gggcgaagaa gcagctgcgc tccatcccca gcggctcgaa gatccgctcg
     1441 cgcatcagga catcgaacgg cttgccgccc cgtacctcca gcacccggcc ggccaggtgg
     1501 aagccggagt tcgtgtaggc ccacagctcg tccggctggt agacctgccg caacgtgtgg
     1561 aactcggcga tcgccttcgc cagcgcatcc tcgccctgac ccatgtcgat gaagaagtcg
     1621 ccgtgcagcc ccgatccatg cgagagcaaa tgccgcagcg tgattgttcg ctggacgttc
     1681 tcgtcggcca gccgcagttc cggcaggtac tccgccactg gcgtgtcgag cgagacgacg
     1741 ccgtcctcgg ccaggatcat cgccagcgtc gccgtgaata ccttcgagat cgacgcgacc
     1801 cgcaccagca tctccggccg catcggctcg accccgttga tattggcgat cccccagctc
     1861 tccagccgga tctcgccctc cttcaggacc ccaatcacga caccggggat ctgccagtac
     1921 tccatctgct gctggaccag ctcgtccagc gtcacgacgt tcgtcgttat cgtcatccat
     1981 cctcccatcg ctcgcgcgaa ttcgaccacg cgcattgtcg gacaggatag cgagtgtcgc
     2041 aatccggacg cgggacgcgg gacgcatcca ccatccctcg tgcctcatcc ccgcatcacg
     2101 ccccgccgtt tcgcgttacc atgccccgcg agggagacgc gcgtgaggag agaagacacc
     2161 catgcggcgg gacgcacgtc agtctgtcga tctggcaacg gtcgatcctg ccgaacctgc
     2221 tccggccggc gtgcggcgtc gtgggccgtt tcgcgtctca ccgcgcgtac tggcgtcccc
     2281 ggcctatcga cgcatctggg tatcgggcat catctactac agcgcccgtt gggtcgagat
     2341 catcacgacc ggctggg
//
LOCUS       DASUGJ010000410         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1731163, whole genome shotgun sequence.
ACCESSION   DASUGJ010000410 DASUGJ010000000
VERSION     DASUGJ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731163"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VFV93_08990"
     CDS             <1..940
                     /locus_tag="VFV93_08990"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEX5165517.1"
                     /translation="ITIIALIITASLLLAGCKDQNGSIGSPIPTATKPAATQSASPLA
                     SGVATEPPTVTATAFATGTATATTSASPTEPVAQGEPPVYGYRIVNSYPHDPAAFTQG
                     LDIDDGALFEGTGLNGESSLRRVNLTTGEVLQQVNLPEEFFGEGIVVFGERIYQITWQ
                     SQTAFVYDRETFEQLETHSYPTEGWGLTTDGERLIMSDGSSTIFFRDPETFAESRHID
                     VHDGEDPVVRLNELEWVNGEIWANVWQTDLIARIDPATGAVVGWIDLTGLLQPEDQAG
                     HKVDVLNGIAVDEATGKLYVTGKLWPALYEIELVAP"
     gene            complement(974..2110)
                     /locus_tag="VFV93_08995"
     CDS             complement(974..2110)
                     /locus_tag="VFV93_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5165518.1"
                     /translation="MPPFGPEDAGSPRAGYRFSRRTLLAAIPGLALIAAGVACGDDDN
                     DDDAVPTATTSAGSGGEATATAPVGDSTPTTAAAASPAADGEWTFTDDRGITITLPKR
                     PERIVAQTTSAAVLWDFGIRPVGVFGPSRLPDGTNDFQAGDIDFDQVEVIGDYGTLDL
                     EKLVQLKPDLYVDLTFGGDSLWYLTEDELTQVEEIVPTLGLSMQKISVLDAIGRFEDL
                     ARSLGVDVDSPELAEVKAGFDAAEADLKAAIEEKPGLTVLVVSPAVDTLYVASPDWMV
                     DLHYFRDLGMDIVSPDVGVDGFWELLSWEQANKYPADLILVDARNDQVVGEVASVGTW
                     EALPAVKAGQLGPWYAGAPYSYARLAPMMIELAGIIRDCDPDLV"
BASE COUNT          429 a          764 c          711 g          363 t
ORIGIN      
        1 catcacgatc atcgccctca tcatcaccgc ttcgctgctc ctcgccggct gcaaggatca
       61 aaacggcagc attgggtcgc caattcccac cgcaaccaaa ccggcggcga cgcagagcgc
      121 atcgccgctg gcgtcgggcg tcgcgaccga gccgccgacc gtcaccgcaa cggccttcgc
      181 gaccgggacg gcgacggcga cgacctccgc cagcccgacc gaaccagtcg cccagggcga
      241 accgccagtc tatggctacc ggatcgtgaa cagctatcca catgacccgg ccgcgttcac
      301 ccaggggctg gatatcgacg acggcgcgct cttcgaggga accggcctca acggcgaatc
      361 ctcgttgcgg cgggtgaacc tgacgaccgg cgaggtgctg caacaggtca acctgcccga
      421 ggagttcttc ggagaaggta tcgtcgtctt cggcgagcgc atctaccaga tcacctggca
      481 gtctcagacg gccttcgtct acgaccgcga gacgttcgag cagctggaga cgcactcgta
      541 cccgaccgag ggctggggac tgacgaccga cggcgagcgg ctgatcatga gcgacggcag
      601 cagcacgatc ttcttccgcg acccggagac cttcgccgaa tcgcgccaca tcgacgtcca
      661 cgacggtgag gatccggtcg tgcgcctcaa cgagctggaa tgggtcaacg gcgagatctg
      721 ggccaacgtc tggcagaccg acctgatcgc tagaatcgac cccgccaccg gcgccgtcgt
      781 cggctggatc gacctgaccg gcctgttgca gcccgaagat caggccggtc acaaggtcga
      841 tgtgctcaac ggcatcgccg tcgatgaggc gaccggcaag ctctacgtga ccggcaagct
      901 ctggccggcg ctgtatgaga tcgagctggt cgcgccgtag ccacagcgcg acctggcttc
      961 aatgacgaac gggttagacc agatccggat cgcagtcgcg gataatgccg gccagttcga
     1021 tcatcatcgg ggctaacctg gcgtagctgt aaggtgcgcc agcgtaccac gggccgagct
     1081 ggccggcctt gacggccggc agggcctccc aggtgccgac cgaggcgact tcgccgacga
     1141 cctgatcgtt gcgcgcgtct accaggatca ggtcggctgg atacttgttg gcctgctccc
     1201 agctgagcag ttcccagaag ccatcgacgc cgacatcggg gctgacgata tccatcccga
     1261 gatcgcggaa gtagtgcaga tcgaccatcc agtcgggcga ggccacgtag agcgtatcga
     1321 cagccggcga gacgacgagg acggtcaagc cgggcttctc ctcgatggcc gccttcagat
     1381 cggcttcggc ggcgtcgaag ccggccttga cctcggccag ttccggcgaa tcgacatcga
     1441 cgcccagcga gcgggccaga tcctcgaagc ggccgatcgc atccaggacc gagatcttct
     1501 gcatcgagag ccccagcgtc ggcacgatct cctcgacctg ggtgagctcg tcctcggtca
     1561 ggtaccagag cgaatcacca ccgaaggtca gatcgacata cagatccggc ttgagctgga
     1621 ccagcttctc caggtcgagc gtgccgtagt cgccaatcac ctcgacctgg tcgaagtcga
     1681 tgtcaccggc ctggaaatcg ttcgtgccgt ccggcagccg cgacgggccg aagacaccaa
     1741 ccgggcggat gccgaagtcc cagaggacag ccgccgaggt ggtctgggcg acgatccgct
     1801 cgggccgctt cggcagcgtg atcgtgatgc cacggtcgtc ggtgaaggtc cattcaccat
     1861 ccgcggccgg gctggccgct gcggccgtcg tcggcgtgct atcacccacc ggagccgttg
     1921 ccgtcgcctc accgcccgat cccgcgctgg tcgtcgccgt cggaaccgca tcgtcatcgt
     1981 tgtcatcgtc accgcaggcg accccggctg cgatcagcgc cagtcccgga atggccgcca
     2041 gcaatgtccg gcgcgagaat cgatagccgg cgcgcggtga tccggcgtcc tccggtccaa
     2101 atggtggcat ccagtcccct cccctgaacg acgaacagat aatcccgact aaaatagtcg
     2161 ggattatcct atgggttgcg tgtcaggctg tcaagcggac ggatcgtgat gcgggacgca
     2221 cacaatcgac aatccggtac gggcgatatc gcaatatcgc cctgctt
//
LOCUS       DASUGJ010000411         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1731223, whole genome shotgun sequence.
ACCESSION   DASUGJ010000411 DASUGJ010000000
VERSION     DASUGJ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731223"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFV93_09000"
     CDS             <1..180
                     /locus_tag="VFV93_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165519.1"
                     /translation="YRNIALRDARDAREGRYCDIARTGLGIVRRSSECLECSIKSGIV
                     VGRQRRWNACIPQRC"
     gene            262..2538
                     /locus_tag="VFV93_09005"
     CDS             262..2538
                     /locus_tag="VFV93_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5165520.1"
                     /translation="MADPESIPQRIGSYEILALLGDGGSGPVYHGIHTQLARPAAIKV
                     LRSDLAEDPNIVRRFQREARIAAQLHHPNIVEIYDTGEAGGVYYIARGLVDGETLRQL
                     LDERRPAIGDSVRIARQIAEALDHAHERGVVHRDLHPGGIVLDRRGHVTVIDFGIAGA
                     VEDLIPVSGDTETMSSWFETPAYVAPEQATGQPAVPASDIYALGAIVYEMLTGEPPVS
                     GTDARELLASVLSETPPLPTERNPALPEGVNDVVMQALAKSPTDRYPTAAEFVADLTR
                     VLGTGELRRPRMGIPPIVLAPETTSATTEMPAGAAPVAAAGAVPEPIVIERVVYRSRR
                     YEPAVWYGVGTTLLAAALLLNLFWNDDNGARADVDVPPPITVTSQTGVVPTATTTPVP
                     ATPTIVATEPAVPTATATAPVPTPTVPPTATPSPTMTPAPPRLPSLATGGELLLFSTE
                     REDVPGGLIVTYPNGADQMPVFSDAAGFTDAAQFAVAPDGSSVAFSAIPVGGDSREIF
                     VYNVAAGEVSQLTDAPGDDLEPRWSPDGQRIAFISNRDVNSEIYVMAVDGSAQTNLSN
                     DTGGDFGHAWSPDGSQIAFGTNRTGDNEVFVVNADGSNVRNLSNNPDADWGPAWSPDG
                     TRIVFGSNRDGSDDIFIMNADGSGQQNLTNDPAADNGAVWSPNGQLIAFVSDRGESSD
                     IYVMTAEGVDVRSIGATIEQEGSPAWSPDSSAIAYTVTIEGNSDIFIAPLEDGTPQRL
                     TDDPGVDAGPIWINIPER"
BASE COUNT          450 a          912 c          839 g          389 t
ORIGIN      
        1 tatcgcaata tcgccctgcg tgatgcccgc gacgccaggg aagggcgata ttgcgatatc
       61 gcccgtaccg gattggggat tgtccgtcgc agctcggaat gccttgaatg cagcatcaaa
      121 tccgggatcg ttgtcggcag acagcgccgc tggaacgcct gcattcccca acgctgttga
      181 agcctttagc ctgttgctgt atactcgtac acagtgtacg tacactcatt gacgggtatc
      241 gcgtgagggc cggcggcagc gatggcggac ccagagtcca tcccacaacg gatcggcagc
      301 tacgagatcc ttgcgctgct cggcgatggt ggcagtgggc cagtctacca cgggatccac
      361 acgcagcttg cccgtccggc cgctatcaag gtcctgcgca gtgatctggc cgaggatccc
      421 aacattgtcc gccgcttcca gcgcgaggcg cggatcgccg cccaactgca tcaccccaac
      481 atcgttgaga tctacgatac cggcgaggcc ggcggcgtct actacatcgc gcgcgggctg
      541 gtcgacggtg agacgctgcg acagttgctc gacgagcgtc gcccggccat aggtgacagc
      601 gttcggatcg cgcggcagat cgctgaagca ctggaccacg cgcacgagcg aggtgtcgtc
      661 caccgcgatc tgcatcccgg cgggatcgtg ctcgaccggc gtgggcatgt caccgtcatc
      721 gacttcggga tcgccggggc tgtcgaggac ctgatcccgg tcagtggcga taccgagacg
      781 atgtcgtcgt ggttcgagac gccggcctac gtcgcgccgg agcaggcgac cggccagccg
      841 gccgtgccgg ccagcgacat ctacgcgctg ggcgcgatcg tctacgagat gctaaccggc
      901 gagccgccgg tcagcgggac cgatgcgcgc gaattgctgg ccagcgtcct gagcgagacg
      961 ccaccgctcc cgacggagcg caacccggcc ctgccggagg gcgtcaacga cgtcgtgatg
     1021 caggcgctgg cgaagagccc gaccgaccgc tacccgaccg ccgccgagtt cgtcgccgac
     1081 ctgacgcgcg tcctcggtac cggcgagcta cgccgcccgc gaatgggcat cccgccgatc
     1141 gtgctggcgc cggagacgac cagcgcaacg accgagatgc cggccggcgc cgcgccggtc
     1201 gcggcggccg gggccgttcc ggagccgatc gtgatcgagc gcgtggtcta ccgcagccgc
     1261 cgctacgaac cggccgtctg gtacggggtt ggcacgacgc tgctggctgc tgcgctgctg
     1321 ctcaacctgt tctggaacga tgacaacggc gcgcgggccg acgtcgatgt cccgccgccg
     1381 atcaccgtca cgagccagac gggtgtggtg ccgaccgcca cgacgacgcc ggttccggca
     1441 acaccgacga tcgttgcgac cgagccggcc gtaccgacgg cgacggcaac tgccccggta
     1501 ccgacgccaa ccgtgccgcc gactgcgacg ccgtcaccaa cgatgacgcc ggccccgccg
     1561 cgactcccca gcctggcaac cggcggcgag ttgctgctgt tcagcaccga acgcgaggac
     1621 gtgccgggcg gactgatcgt gacctacccc aacggcgcgg accagatgcc ggtcttcagc
     1681 gacgccgccg gcttcacgga cgcggcccaa ttcgccgtcg cgcccgacgg cagcagcgtc
     1741 gccttctcgg ccatccccgt cggtggcgac agccgcgaga tcttcgtcta caacgtcgca
     1801 gccggtgagg tcagccagtt gaccgacgcc cccggcgatg acctggaacc gcgctggtcg
     1861 ccggacggcc agcggatcgc gttcatctcg aaccgggatg tcaacagcga gatctacgtg
     1921 atggctgtcg acggcagcgc tcagacgaac ctctccaacg acaccggcgg cgatttcggc
     1981 cacgcctggt cacccgacgg cagccagatc gccttcggca ccaaccgcac cggcgacaac
     2041 gaggtattcg tcgtcaatgc cgacggctcg aacgtccgca acctcagcaa caaccccgac
     2101 gcggactggg ggccggcctg gtcgccggat ggcacccgga tcgtcttcgg ctcgaaccgc
     2161 gacggcagcg acgacatctt catcatgaac gccgacggct ccggccagca gaacctgacc
     2221 aacgatccgg ccgccgacaa cggcgcggtc tggtcgccga acggccagtt gatcgcgttc
     2281 gtcagcgatc ggggcgaatc gtccgacatt tacgtgatga cagccgaagg cgtcgatgtt
     2341 cgctcgatcg gcgcgacgat cgagcaggag ggctcaccag cctggtcgcc ggattcgagc
     2401 gccatcgcct acaccgtcac gatcgagggc aatagcgaca tcttcatcgc cccgctcgaa
     2461 gacggcaccc cgcaacggct gaccgacgat cccggcgtcg atgccggccc gatctggatc
     2521 aatattccgg aacggtaagt tcgccctgcc cgtctcgcgt aggggcgatg gccgcagcct
     2581 cgccctgctt
//
LOCUS       DASUGJ010000412         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1036302, whole genome shotgun sequence.
ACCESSION   DASUGJ010000412 DASUGJ010000000
VERSION     DASUGJ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036302"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFV93_09010"
     CDS             <1..614
                     /locus_tag="VFV93_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5165521.1"
                     /translation="LLGGSIHEQSDTPGMFYNTSVLFDPAGEIIAKYRKIHLFDIDLT
                     GNVTANESSTILPGDEIVTADVDGHTVGLSICYDLRFPELYRQLALDGAEILLVPAAF
                     TMFTGKDHWHVLLRARAIENQTYVLAAGQFGTHGENNAQCFGHSIIIDPWGTVIADSS
                     DSEGVVVADLDFAYLRKIRKQLPSLANRRPSAYRERVAAGIAD"
     gene            987..2459
                     /locus_tag="VFV93_09015"
     CDS             987..2459
                     /locus_tag="VFV93_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165522.1"
                     /translation="MRVVVALGFVGVLLLATWNLGRYPIISGWDEGIYLEFASNLANH
                     GAYASNEAGTFNHLTPVGGTGPTIILPVAAAISIGDGDLAVARLPMALFLIFMVVSAC
                     LLARTIGATGAALAVIPLILTAGYKSIDTLWLGRQVLADVPALALMLFGLWCWIRSWS
                     GGWRWLISASMFLGLSVVTKNQMILALGLALVVVAIVDRLYYRQLQWRHSIVPVISVG
                     GCYLLWVIASLVIVGADGRDTYLENQRALSQAQFLNVSPSRISMNAKLLFADGQWLIA
                     LIAIGICLIASRQRTMDGLRRFVLPLITAISLVAFVGMSVPWGRLLYVPLALTAICVA
                     VAVSDSLRWLDAVRGQNWVRNGVIGLVVVAALTGTRLLDDVRLIRTTDDRHAEEFAVL
                     IDSTIPHDAIIHNWEWEIDFHSSHTFSHPDFRLFPALINEVYGGPSHPIVGEPRIPAV
                     EYVIVGPFATQTAVFADALDERDATVRLQHGPYALYQLSR"
     gene            complement(2487..>2693)
                     /locus_tag="VFV93_09020"
     CDS             complement(2487..>2693)
                     /locus_tag="VFV93_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5165523.1"
                     /translation="ESGLSFLGLGPPPDIPSWGRSIQQSSRFMSQHPWGVVWPAIVIS
                     AAVLAFNILGDALRDRLDPRLKSG"
BASE COUNT          452 a          822 c          848 g          571 t
ORIGIN      
        1 tcctgctggg cggctcgatc cacgagcagt cggacacgcc gggcatgttc tacaacacgt
       61 cggttctctt cgatccggcc ggtgagatca tcgcgaagta tcggaagatc cacctgttcg
      121 acatcgacct gaccggcaac gtcactgcca acgagtcgtc gacgatcctg ccgggcgatg
      181 agatcgtgac ggccgatgtc gacggccaca cggtcggtct gtcgatctgc tacgacctgc
      241 gcttcccgga gctgtaccgg cagctggcgc tcgacggcgc cgagatcctg ctcgtgccgg
      301 ccgccttcac gatgttcacc ggcaaggacc actggcacgt gctgttgcgc gcccgtgcga
      361 tcgagaatca gacctacgtc ctggccgccg gccagttcgg cacccacggt gagaacaacg
      421 cgcagtgctt cggtcacagc atcatcatcg acccgtgggg caccgtcatc gccgactcgt
      481 cagacagcga gggcgtcgtc gtcgccgacc tcgacttcgc ctacctgcgc aagatccgca
      541 agcagctccc gtcgctggcc aaccgccgcc cgagcgccta ccgcgaacgt gtcgccgccg
      601 ggattgcgga ttaggcctcg cgcgggacaa cgcgcgagga cacgacatcg gcgtttcatc
      661 aggcgcacgt cgagatgaac accaccaagg gcacaacccc cggtggtgtt catcgtggat
      721 tccttcgcct gctcgtctga aaccgttgcg tgtcgttcct gggcaagcat cgccaccctg
      781 tgagcgaaga tgaccggaat gtcaccacgt ttcgccggtt tggcggggtg gagtccagtt
      841 gctgcatgtc tactttgagc atctcatgtc agtttcgacg cgcgtgtgat gcaagaggcg
      901 ccatgaagta ttaccgcccc gagtatgcgg acgttccagg ctggctctcg ttccgatctc
      961 tcgcaactcg cgatcgtagt gcggccatgc gcgttgtcgt cgcactcggc ttcgtcggcg
     1021 tgctgctgct cgcgacctgg aacctgggac gatacccgat catcagcggt tgggacgaag
     1081 ggatttacct tgagttcgcc tcgaatctgg cgaaccacgg tgcatacgcg tcgaacgagg
     1141 ccggcacctt taatcatctg acgccagtcg gaggtacggg accaaccatc attctgccgg
     1201 ttgctgccgc tatttcgatc ggtgatggtg accttgcggt ggcacggttg ccgatggcgc
     1261 tcttcctgat cttcatggtc gtcagtgcct gcctgctggc tcgaacgatt ggcgcaacgg
     1321 gcgccgccct cgctgttatc ccactcattc tcacggcagg atacaagtcg attgacacgt
     1381 tgtggcttgg gcgacaggta cttgccgatg ttccggcgct ggcgttgatg cttttcggac
     1441 tgtggtgctg gatacgaagc tggagcgggg gttggcgctg gctgatatcc gcgagtatgt
     1501 tcctcggttt gtccgtcgtc acgaagaacc agatgattct cgcgctcggg ctcgcgttgg
     1561 tcgtcgtggc cattgttgac cggctgtact atcgccagct ccagtggcgg cattcgatcg
     1621 tcccggtgat atccgtcggt ggctgctatc tgctctgggt cattgcgtca ctcgtcatcg
     1681 tcggcgctga cggccgggat acctatctcg agaaccagcg cgcgctgtca caggcacagt
     1741 ttctcaatgt cagcccgtcc cgaatcagca tgaacgccaa actgttgttt gcggatggcc
     1801 agtggttgat cgcactgatc gcaatcggca tctgcctgat cgccagccgt cagcgcacga
     1861 tggacggcct gcgacggttc gttctcccgc tcatcaccgc gatctcgctt gtggcgtttg
     1921 ttggcatgag cgtcccgtgg ggtcgattgc tgtatgtgcc gctggcgctg acggccatct
     1981 gcgttgcagt ggcggtatcc gacagcttgc gctggctcga tgccgtacgg gggcagaact
     2041 gggtccgcaa tggcgtgatc gggctcgtcg tcgttgccgc cctgacgggt acccggctcc
     2101 tggacgatgt gcgcttgatc cgcacgactg atgatcgtca cgccgaagag ttcgcggtgc
     2161 tgatcgattc aacgatccct catgatgcga tcatccacaa ttgggagtgg gagatcgatt
     2221 tccacagcag ccacacgttc agtcatcccg acttcaggtt gtttccggca ctgatcaacg
     2281 aagtctatgg tggaccgagc cacccgattg tcggcgagcc acggataccg gctgtcgaat
     2341 acgtgattgt tggcccgttt gccacgcaga ccgccgtgtt tgcggatgcg ctggatgagc
     2401 gcgatgcgac cgttcgactt cagcacggac cgtacgcgct ctaccagctc tcgcgatagc
     2461 cgggcgcttg ctacggaaga acgatctcaa cctgacttga ggcgtggatc gagccggtcg
     2521 cggagcgcgt cgccgaggat gttgaaggcg agcaccgccg ccgagatgac gatggctggc
     2581 cagacgacgc cccaggggtg ctggctcatg aagcggctgg actgctggat cgagcggccc
     2641 caggagggga tgtcgggggg tgggccgagg ccgaggaagc tcaggccgga ttc
//
LOCUS       DASUGJ010000413        17118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1731796, whole genome shotgun sequence.
ACCESSION   DASUGJ010000413 DASUGJ010000000
VERSION     DASUGJ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 17118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17118
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731796"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..1206
                     /locus_tag="VFV93_09025"
     CDS             466..1206
                     /locus_tag="VFV93_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5165524.1"
                     /translation="MDQASIEDRAPVTVVLVHGAFADSSSWTGVIERLQAVGVRVIAP
                     PNPLRGAAHDAAYIASFLDQISGPVLVVGHSYGGVVITNAASMAKNVVGLIYVAAFAP
                     DEGETLLEIESDSRDSVLVSALLQYQYPTEPGAETVVEFVVDPDKFHEAFAADLPMRQ
                     TAVMAATQRPASLLAFSEPTGTPAWKSLPSWAVVATGDKAAGSDVVRQMAKRAGATTT
                     EVEGSHAIMLSQPQVVTDMILTALNSVS"
     gene            1248..2012
                     /locus_tag="VFV93_09030"
     CDS             1248..2012
                     /locus_tag="VFV93_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5165525.1"
                     /translation="MKIVVIGGTGLIGSKLVAKLQEHGHEAIPASPRLGINSITGEGL
                     AEVMSDASVVVDVSNAPSWEDSAVLKFFETSTRNLLAAEAKAGVGHHVALSVVGTDRL
                     AESGYFRAKIAQEKLIREASIPYTIVHATQFFEFMQSIAEAATEGDIVRLPSVYIQPM
                     AAEDVASAVGRVAVGAPANGIVEVAGPDRFLLDELIRGRLSASGDPRMVITDPNASYY
                     GINPGLRVLLPGDGAQIAETHYADWLKQSAEAVAAR"
     gene            2200..3030
                     /locus_tag="VFV93_09035"
     CDS             2200..3030
                     /locus_tag="VFV93_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5165526.1"
                     /translation="MAVRTDTHAHLVWPIFLLAAAGLLLLSLSPVTAEAQRIGRHGNQ
                     PTIVLVHGAWADPSGWQQVIDRLHKDGFQTVTPKLDLLSLDGDTAIVRAALDAITGDK
                     ILVGHSYGGEVVSAASAGRSDIRAIVYTAAFVPDQGETGLDLLAGYAPSAALDHLIFT
                     GEPWNSPAYLDSAFFHEVFAADLSDSDAAAMNAAQQPTHPATFASPSGPVGWHDLPVY
                     YAVSGQDLIIDPNLQGWMAARAGATTIEFKKASHVGGFTVHAHQFTKLIERAVRETGC
                     "
     gene            3361..6195
                     /locus_tag="VFV93_09040"
     CDS             3361..6195
                     /locus_tag="VFV93_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5165527.1"
                     /translation="MAVLGAIGTAPLVDRVAERQLIDQRLTAAASAPGSVIVISGEAG
                     VGKTRLASATAAGPDRTVVHLRCDEQLHDVPYAPFLDPVGGLNELPAIVTSYRSAVDS
                     PVARFELYEQVDRLLGEQAEGTTGVLVIDQLEWIDAASLDLLRHLVRRGHRGGRAILA
                     TMRAGVTPLDSPLGQLMADWNRERLLLDVPLAPLDRPSSDKLINDLLGSVDLELCDTI
                     YARTDGLPFFIEEFVRLLVTEGHASRSGGIWRSARSAAGDSSVSFGIAATVLRRLDQL
                     PAETRQALRAASVLGARCSLSILAALMGCAEAELIEELSPAVASRLFRLERHDARDFP
                     SHGEFEHALVRDALYAALPREDRIALHWRVADLLAHAPSERSYDLATSADAALLAFHA
                     ERAHLWTLAYEASLAAGDEAVSVLAGRDALTHFRQARRLARSGHVSTSADDALALDRR
                     MVTTLSGIGSLQEAATAAREMARRASASGDHVAEAWAWIQFAGARTYSDRLERVEADL
                     EQGKSIADALGDASLLATALATRGTIRGARGLLDSAEQDFHEAIALAERIDNQVITLK
                     GLIHVGVTASWRGRFHDAIATGKQAAELAEASHNSSALVDARYTLALALAGTGEYEEA
                     LGCLEFLLGLARTSGEAYYAARVPNTIGWIYRELALVEQALPWDERAVQESEQVGGIC
                     HFKAHANSLLNLVLDLILRDRLDDAEVALVRADEAVNQMEYMRWRTATRLALCRGELA
                     LARGNDVLALSLADDALARATATQSAKHVHQAHDLAGRALLASARYQDAIGRLDQAVS
                     LAGAIDYPAGHWRSLAHLADALSQQGQPNAADQCLAEAAGVIETIATRLRNVELRDAF
                     LAAREVARLLSRTAIGATAPAASHPMDLSAREVEVLRLVAEGLTNAQIAEQLFLSPKT
                     VSSHLGSIFSKIGVTSRASATRFAIEHNLI"
     gene            6365..7345
                     /locus_tag="VFV93_09045"
     CDS             6365..7345
                     /locus_tag="VFV93_09045"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5165528.1"
                     /translation="MTTALPATVPTVVLESAAQALADALTAGGGPPLYTLTPQEARAV
                     LDGAQAGTIPMAPAMIEERIVPGGPGGQVLITTVRPVGMAHVLPGVIYIHGGGWVLGN
                     FATHERLVRELAEQTGAVYIFVNYTPSPEAQYPVAIEEAYAALTWVAEHGAEIGVDPS
                     RLAIAGDSVGGNMTAAVALLANERGGPALRLQALFYPVTNAAFTTDSYDQFADGPWLT
                     RKAMQWFWDLYAPDLARRMEPTASPLLASLEQLAGLPPALVITDEADVLRDEGEAYSR
                     KLRQAGVDVTAVRYEGIFHDFMMLNALAETNATQAATAQAALAMKAALVP"
     gene            7442..7864
                     /locus_tag="VFV93_09050"
     CDS             7442..7864
                     /locus_tag="VFV93_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165529.1"
                     /translation="METEAGTLTLDPPVQSRQTYPYHTLITSVPHAEARVVAAALVAA
                     GFTRDRFEITTAEEISWLGERIGASGAYGLLNRLRFSLGDALVAEGPARLGLLEQYAL
                     VQILIYNDQEQARARTILDQYGGHLMHYFGRWTVSDGD"
     gene            complement(7962..8849)
                     /locus_tag="VFV93_09055"
     CDS             complement(7962..8849)
                     /locus_tag="VFV93_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165530.1"
                     /translation="MLSRSSTEEPSGRRGALFPDWLPSRGGLVLALLLLVMPLVIALA
                     VTTTSGPLFGAWGWAEFALVLLMGEVVLLLTLGMYRGEHNPYRTVVPSASHWGSHTND
                     PLVIYAGTLRPPIAADARRSGNNHPILLAIAPAIGALAMLGVVVFGPENDAATTPPEP
                     AALPIVAVDQRLSLMLESVELQSNAMTLSIRLDDIELDRSTAGYVVPQVDDVTMNGML
                     PDRIDASFDAIHRIHQPGYAGVTTWRMHVVIAPPPADASQPLTVTFHTIRFAPNHDIP
                     KLPSQVDGNWSFTFIPATS"
     gene            complement(8994..10196)
                     /gene="cysS"
                     /locus_tag="VFV93_09060"
     CDS             complement(8994..10196)
                     /gene="cysS"
                     /locus_tag="VFV93_09060"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5165531.1"
                     /translation="MLKLYDTLSGEKREFKPLDGRTVKMYVCGVTPYDTTHIGHAMTY
                     LTFDVVNRYCQYLGWRVRYVQNVTDIDDDILKRAKRDNEEWDLLGDRHTKQLVEDLSA
                     LNCLWPQAYPRASEETEKIIEITAALIADGYAYESQGNVYFRVANDEDYGALGKYEWD
                     EMIELSRERGADPDDPRKEGPLDFILWQGQQPGEPAWESPWGPGRPGWHVECSAMAMR
                     YLGPKIDIHGGGSDLIYPHHECEIAQSQHASGEEPFCQFWMHIGMVRYQGEKMSKSLG
                     NLVLARNVLRVHTPDALRLYLHSNHYREAWSYRDTGPAEYEGLASMLKEAARLDSRHG
                     DEFDGAGFETRFRGALDDDLNTPEAVHVLRDFAADIITTAAEGRNVRAAQARLRTLSS
                     ILGVQGVR"
     gene            10396..11271
                     /locus_tag="VFV93_09065"
     CDS             10396..11271
                     /locus_tag="VFV93_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5165532.1"
                     /translation="MTESDNTSEQPEGGTRSRRRGRGVRPHWADTPQMRENPSLNARE
                     RMGQPTEDEQFLESKTREEAAAAFIHTDPWRVLRIMGEFVQGFDTLASVGKAVTIFGS
                     ARVKEDDPMYAAARELASRLAGEGFAIITGGGPGIMEAANRGAVDAEGQSIGCNIELP
                     FEQGMNQYVEVAVNFRYFFVRKTMFVKYAEAFVIFPGGFGTLDELFEALTLIQTGKIH
                     NFPIVLFGSDYWQGLLDWTKNTLLAEGKISADDLKLLAVTDSAEDACRIIVECYDNQC
                     WLAEDHSAAARLGIR"
     gene            11438..13588
                     /locus_tag="VFV93_09070"
     CDS             11438..13588
                     /locus_tag="VFV93_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5165533.1"
                     /translation="MQVAGPGIGRLSRARISAALVVAIVLSLSLGTLPVRAASVPRSG
                     PHQLIVTFRSSSPYWAPYETAEGLALDAVARADHGLRGQRDLAERRDLSLLTYADAAS
                     ASRAMETLRRNPDVLAAEPNVERQLAWVPDDDAYSEQSWWLDLINAPDGWNINTGSDD
                     TIVAVIDSGVSPTHPDLSGRLVPGYNVVDGTTDTSDIAGHGTHVAGIIAAQQNGIGTV
                     GVAMGVHIMPIRVVDGAGDIDVSNEIEAIYWAVDNGADVINLSLGAEEYVQAEREAIQ
                     YAYNRGVVVVAAAGNYFNKVSYPGNYDEVIAAGSLKADGYPAPFTSRITRVDVSAPGE
                     RIYSPGWDDFFGDYWDDVFYADNSPVSGTSFSSAIVAGVVALLKSIDPTAGTEQVRSL
                     LKSTATDTGGIGVAEAGAGAGEVNVEAALRASLDLELNETWHRADEPVASGAVSRTWL
                     WGQEASETLYEPYDETQHGTRLVAYYDKSRMEITDPMEDRTQQWYVTNGLLVVEMITG
                     NLQLGDDHFVSHGASNVNIAGDPDDTSGPTYAAFAGLLNLPTVMQDQTLILTVDRTGR
                     VNANSHLATYGVTGDQYVPETKHRIANVFWDYLNSVGPIANGSDLTDGQLFDPWFYAT
                     GYPITEAYWARVKVRGIIQDVLIQCFERRCLTYTPANAEGWKVEMGNVGLHYYDWRYN
                     SSATLDPNLTIRVPATANPGPRQSLRQLWRTGFN"
     gene            14079..16847
                     /locus_tag="VFV93_09075"
     CDS             14079..16847
                     /locus_tag="VFV93_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5165534.1"
                     /translation="MTHVLRHPMAAVVVLALLLSTVTPVLAIGSNPPEPDGPGSGAEL
                     LAGAEKLKHPTLDSSLFKIARDVRAGKQPKDAIARQAKVSRGESVAVSVDVAGDPAAT
                     VAWLNSHGAQVDNVGQRLIEAYVPVAELESLGSLSGVLAVRSMRPPFASVMSQGVALH
                     GVPNWNTNGYTGAGVKVGVIDVGFDGIVSLVGSELPQLTARCYPTSGAVTSNAQDCDR
                     NGVHGAAVAETVIDVAPGASLYISNPRSWLELNATVNWMISQGVTVINHSVGWGWEGP
                     GDGTTIYADSPLGAVDRAVAGGITWVNAAGNEGLATWSGDYLDSNNDNIMEFASGGVD
                     LNSVPAYSGQSIDLMMRWDDSWPRATSDLDLYLLDSFGNIVATSQEQQNGGSGDTPIE
                     ILSYMPSDSGYLYIAVEQFGGADPDWVQIQEFNGLPLGLSSPEYSIANPAESANPGLL
                     AVGAASWFSPSTIEDFSSQGPTRDGRIKPDVVGIDQADTVSYGPSGFPGTSQASPHVA
                     GLAVLVKQRFPQYSAAEVAQYLRSNASQAQAPNNVWGYGLAYLPEIVAGENPAPAISS
                     ISPATVQLGSYPLTITVNGNGFVPESVVHWNGEDRATTYVSPSQLTFEVDYRDTDVAS
                     TVEITVSSPGPGGGVSNAVTLTIDDDTPQVPNAVDYPEFLRTWQRTDEPVKQLVAQRT
                     WMWGDGQASDAMVEAYADAPGGYRSVKYFDKSRMEVNDPNEDNTQSWYVTNGLLSKEL
                     ITGQMQMGDDAFVDHGPAEVPVAGDPNDANGPTYATFTDLLDAAPAQSGSMLTQRIDR
                     NGTVTDDPSLAQYGVTAASLVEVPGIRHQVASPFWEFMNSTGTIYANGQYLTSQLFDP
                     WFYATGLPITEAYWATVKVNNQYVDVLMQCFERRCLTYTPGNPDGWKVEAGNVGLHYY
                     VWRYGMMP"
     gene            complement(16935..>17118)
                     /locus_tag="VFV93_09080"
     CDS             complement(16935..>17118)
                     /locus_tag="VFV93_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165535.1"
                     /translation="LPITEAYWATVKVNNQYVDVLMQCFERRCLTYTPGNPDGWKVEA
                     GNVGLHYYVWRYGMMP"
BASE COUNT         2954 a         5575 c         5580 g         3009 t
ORIGIN      
        1 ctacgtcggt gactcgtacg accacgacgt gatcggcgcc cgcgcggccg gcctgacgcc
       61 ggtcctgatc gaccgcttcc tgatcaacct cgacagccac gacgccgaac accgcgtcga
      121 gacgctccac gacctggccg acctgctgga ccggctcatt cactcctgag ctgaatcccg
      181 gtcgccctcc gttgctcgtg actcaatcca atccccatat gagcttcgat caggtattcg
      241 cctgatctcc cttgccgtgt tcgtcaccgg actgaacatt ggatttcggg cgatcacccg
      301 atgtggttcg ggggtcagac gcctagagtc gatgcatgcc atcgggacct cagcggaccg
      361 gaacggcgga gtagagaaag gagatagccc gacaaccgca acgcccgcag gtccgtggcg
      421 gccaatcgtt cgtcgcattc aacatcctcg aaaggaacca ccatgatgga ccaagcgtcc
      481 attgaagatc gcgcacctgt cactgtcgtg ctcgtccacg gtgccttcgc cgactcgtcg
      541 agctggactg gtgtcatcga gcggctccag gcagtcggcg tgcgggtgat cgcgccaccc
      601 aacccgctgc gtggcgcggc acacgatgct gcctacatcg ccagctttct cgaccagatc
      661 tcggggccgg tcctcgtcgt cggccactca tacggtggcg ttgtcatcac caacgctgcc
      721 tcgatggcga agaacgtcgt cggactcatt tacgtcgcgg ccttcgctcc ggacgaaggc
      781 gaaacgctgc tggagatcga aagcgactcg cgggacagcg tcctggtctc ggcgctgctc
      841 cagtatcagt atccaaccga accgggcgct gagacggttg tcgaattcgt tgtcgatccc
      901 gacaagttcc acgaagcctt cgctgctgac ctgccgatgc ggcagacggc cgtgatggcg
      961 gccacccagc ggccggcatc gttgctggcg ttctcggagc cgaccggcac gccggcctgg
     1021 aagtcgttgc cgtcctgggc agtcgtcgcg accggtgaca aggcggccgg cagcgatgtc
     1081 gtgcgccaga tggcgaagcg tgccggggcg acgacaaccg aggtcgaagg ctcccacgcg
     1141 atcatgctct cgcagccgca ggtcgtgacc gacatgatcc tgacggcgct caattcggtg
     1201 tcgtagccgg cgtaacctgt caaccctcca tcatgaaagg aagaccaatg aagatcgtcg
     1261 ttatcggtgg gaccggcctg atcggatcga agctcgtcgc caagctccag gagcacgggc
     1321 atgaggctat cccggcatcg ccccggctgg ggatcaactc gatcaccggc gaggggctgg
     1381 ccgaggtgat gtccgacgca tcggttgtcg tcgacgtgtc gaacgcgccg tcctgggagg
     1441 attccgccgt cctcaagttc ttcgagactt cgacgcgtaa cctgctggcg gccgaagcga
     1501 aagccggtgt cggccatcac gtcgcgttgt cggtcgtcgg gaccgatcgg ctggccgaga
     1561 gtggctactt ccgcgccaag atcgcgcagg agaagctgat ccgggaagcg tcgattccct
     1621 acacgatcgt tcacgcgaca cagttcttcg agttcatgca gagcatcgcc gaagccgcca
     1681 ccgaaggtga catcgttcgg ctcccgtcgg tgtacatcca gccgatggct gccgaagatg
     1741 tcgccagcgc ggtcgggcgg gttgccgtcg gcgcgccagc gaacggcatc gtcgaggtcg
     1801 ccggtcctga ccggttcctg ctggacgagc tgattcgtgg ccgcctcagc gcctccggcg
     1861 atccgcgcat ggtgattacc gatcccaacg cgagctacta cgggatcaat ccgggcctgc
     1921 gggtgctcct gcccggcgat ggcgcccaga tcgctgagac gcattacgcg gactggctta
     1981 agcagtcggc cgaagccgtc gccgcccgct agcgactcgc gcagcacgga cgagcgattt
     2041 catcccccga ctacgcaggt tcagcgcttg ccggttccac atggggccag gcctggcggc
     2101 tgcattcgcg aaacgacgga tggccgcaac tggcggccac cactcacagc agataatagg
     2161 aggactcctg tgaaccaccg accgatagca cagtcccggt tggcagtccg aactgacacc
     2221 catgcgcatc tcgtctggcc gatcttcctg ctcgccgctg ccgggttgct acttttgtcg
     2281 ctcagtccgg tgacagccga ggcccagcgg atcgggcgac acggcaacca gccgacgatc
     2341 gtcctcgttc acggtgcctg ggccgatcca tcgggctggc agcaggtgat cgatcggctc
     2401 cacaaggacg gctttcagac cgtgacgccg aagctggacc tgctctcact tgatggagat
     2461 accgccatcg tccgtgcggc attggacgca atcactggcg acaagatcct cgttgggcat
     2521 tcctacggcg gggaagtcgt ctcggctgca tcggctgggc gatccgacat tcgcgccatc
     2581 gtctatacgg cggccttcgt tcctgaccag ggcgagacgg gtttggatct gctcgccgga
     2641 tatgctcctt cggctgcgct tgaccacctt attttcaccg gcgagccgtg gaattcgccg
     2701 gcctatctcg actcggcctt ctttcacgag gtcttcgcag ccgacctctc ggattccgac
     2761 gctgcggcga tgaacgctgc ccagcaaccg acgcacccgg cgacgttcgc ctcaccgtcc
     2821 gggccggtcg gctggcacga tctaccggtg tactatgctg tgtcgggcca ggacttgatc
     2881 atcgatccga acctgcaggg ctggatggcc gcgcgggccg gcgcgacgac gatcgagttc
     2941 aagaaggcca gccatgtcgg tggctttacc gttcacgcgc accagttcac gaagctgatc
     3001 gaacgcgccg tgcgtgaaac cggctgctga ccatcacgtg cccaaccggc ccatgccctt
     3061 tctgggcggg gccggttttt cctggttgcc gccgctgccg aatcaaccct ccgcaacagc
     3121 attcgctcct gtttagacac gattgcggtc cgagcgttca cctgatatcg tgatttggat
     3181 gcgcccgctt ccggccaagg caacgatcaa cttctggccg gacgatccgt gttgcgttgg
     3241 gaccggacgc gttcttcagc agccgtggtt catcgctcga gcctcggagg attgcccgtg
     3301 ctagatgacg acaccaatag ccgagcgagg caagacgaag tgacggatac tggcgcagtc
     3361 atggctgttc tgggagccat tgggacagcg ccactggtag accgagtggc cgagcggcag
     3421 ttgattgacc agcggctcac cgctgcggcc agcgctcccg gtagtgtgat cgttatcagc
     3481 ggcgaggctg gagtggggaa gactcggctg gcctctgcga ccgccgctgg cccggatcgg
     3541 accgtcgtcc atctgcgctg tgacgagcaa ctgcacgatg ttccctacgc gccattcctg
     3601 gaccccgtcg gcgggctcaa cgaattgccg gccatcgtga cctcgtacag atccgctgtt
     3661 gattcaccgg ttgcccgatt cgagctgtac gagcaggtcg atcgtctcct cggagagcag
     3721 gctgagggaa cgaccggcgt gttggtgatc gatcagctgg agtggattga cgctgcctcg
     3781 ctcgatctgc tgcggcatct ggtgcggcgg ggccatcgcg gcggtcgtgc catcctggcg
     3841 acgatgcgcg ccggtgtaac cccgcttgac tcgccattgg ggcaactgat ggccgactgg
     3901 aaccgcgaac gtctgctatt ggacgtgccg ctcgctccgc tggaccgtcc atcctccgac
     3961 aagctgatca acgatctgct cggttcggtc gatctcgagt tgtgcgatac catctatgca
     4021 cgaaccgatg ggctcccgtt ctttatcgaa gagtttgtcc ggctgctggt caccgaagga
     4081 cacgcgagcc gctccggtgg tatctggcgc agcgcgaggt cggcggctgg cgatagcagc
     4141 gtatcgttcg gcatcgccgc gacggtattg cgccggctcg atcagcttcc agcggagacg
     4201 cgccaggcgc tccgtgccgc ctcggtcctt ggtgcgcgct gctcgctgag tatccttgcc
     4261 gcactgatgg gttgcgctga agctgaactg atcgaggaac tctctccggc agtcgcttcg
     4321 cggctcttcc ggctcgaacg ccacgacgcg cgcgacttcc cctctcatgg cgagtttgag
     4381 catgctctcg tccgggatgc actctatgcc gcgctgccgc gcgaggaccg gatcgcgctt
     4441 cactggcgtg tggcggatct gctggcacac gctccatccg agcgatcgta tgatctggca
     4501 acgagcgcgg acgccgcatt gctcgcattc catgccgaac gggcgcacct gtggacgctt
     4561 gcctacgagg catcgctggc cgccggtgac gaggcagtgt ccgtcctggc cggtcgcgat
     4621 gcgttgacgc acttccgaca ggccaggcgg ctggcgcgat ccgggcatgt ctcgacatcg
     4681 gcggacgatg cgctggcgtt ggatcgtcgg atggtcacga cgctgtcggg cataggcagc
     4741 ctccaggagg cagcgacggc agctcgtgag atggcgcggc gcgcctcggc cagcggcgat
     4801 cacgtggccg aggcttgggc gtggattcag ttcgccggtg cgcgcaccta ctccgaccga
     4861 ctggaacgcg tcgaagcgga cctggaacag ggtaaatcca tcgcggacgc gctcggcgat
     4921 gcgagcctgc tggcgaccgc tctggcaacg cgcggcacaa ttcgaggcgc ccgtgggctg
     4981 ctcgattccg ccgagcagga ctttcacgag gcgatcgcgc tggcagagcg gatcgacaac
     5041 caggtgatca cgctgaaggg gctgatccat gtcggcgtga ctgcgtcgtg gcgtggccgg
     5101 tttcacgatg cgatcgcgac cggcaagcag gccgccgaac tggccgaagc gtcgcataac
     5161 tcatcggcgc tcgtggacgc gcgctacacg ctggcgctgg cactggccgg aactggtgag
     5221 tacgaggagg cgctcggttg tctggagttc ctgctcggtc tcgccaggac atcgggcgag
     5281 gcctactacg cggcccgcgt ccccaacacg atcggctgga tctaccgcga gctggcgctg
     5341 gttgaacagg ccctgccgtg ggatgagcgg gccgtccaag agtcggagca ggtcggcggg
     5401 atctgtcact tcaaggcgca cgccaacagc ctcctcaacc ttgtgctgga cctcatcctc
     5461 cgcgaccggc tcgatgacgc cgaggtggcg ctggttcgcg ccgacgaggc ggtcaaccag
     5521 atggaataca tgcgttggcg gaccgcgacg cgcctcgcgc tttgccgggg cgagttggcg
     5581 ctggcacgcg gcaacgatgt gctcgccctc agcctggccg acgacgcgct ggcacgggcg
     5641 acggcaacgc agtcggccaa gcacgtccac caggcgcacg acctggctgg gcgcgccctt
     5701 ctcgcgtcag cgcgctatca ggacgcgatc gggcggctgg atcaggctgt ctcgctggct
     5761 ggcgcgatcg actacccggc cggccattgg cgtagcctcg cccatcttgc ggacgcgctc
     5821 agccagcagg ggcagccgaa cgcagccgat cagtgcctcg ccgaggcagc cggtgtgatc
     5881 gagacaatcg ctacacgtct gcgcaacgtc gagctgcggg acgcgttcct ggccgcgcgg
     5941 gaggttgcga gactactgtc gcgcacagca atcggagcga cggctccggc agcctcccat
     6001 ccgatggatc tcagcgcccg cgaagttgaa gttcttcggc tcgtcgctga gggactcacc
     6061 aacgctcaga ttgccgaaca gctcttcctc agcccgaaga cggtcagctc ccacctcggc
     6121 agtatcttca gcaagattgg cgtgacctcg cgcgccagcg ccacccgctt cgcgatcgag
     6181 cacaacctga tctgatcagg cattcgccct agccccgttt aatactcagc ttccgcgaca
     6241 gcaacatcga ttgcggtgtt cgcccgatgt ggtttggccg tcggcggtct attgtcgtgt
     6301 catgcccggc gccgggactg ctgaatccga agcaccagac agggatagga gtctcacggt
     6361 gacgatgaca accgcactcc ccgcgacagt tccaaccgtc gtgctggaat ccgctgcgca
     6421 ggcgctcgcc gatgcgctga cggccggggg cggcccgccg ctgtacacgc tcacgccaca
     6481 ggaagcgcgc gccgtcctcg atggcgcgca ggccggcacg atcccgatgg cgccggcgat
     6541 gattgaggag cgtatcgttc cgggcggtcc cggcggtcag gtcctcatca cgaccgtgcg
     6601 gccagtcggc atggcccacg tgctgccggg cgtcatctac atccacggtg gtggctgggt
     6661 gcttggcaac ttcgccaccc acgagcggct cgttcgggag ctggccgagc agacgggcgc
     6721 cgtctacatc tttgtgaatt acacgccgtc gccggaagcg cagtatccgg tcgcgatcga
     6781 agaagcctac gccgcgctga catgggtggc cgagcatggg gccgagatcg gtgtcgaccc
     6841 atcccggcta gcgatcgccg gtgacagcgt cggcggcaac atgacagcag ccgtggcgct
     6901 gctggcgaat gagcgcggcg gcccggcgct gcgccttcag gcgctgtttt atccggtgac
     6961 caatgccgcc ttcaccaccg acagctacga ccagttcgcc gacggcccgt ggctgacccg
     7021 caaggcgatg cagtggttct gggatctgta cgcccccgat ctcgcccgac ggatggaacc
     7081 gaccgcctca ccattgctgg cgtcgcttga gcaattggcg ggcttgccac cggcgctggt
     7141 tatcaccgac gaggcggacg tgttgcgcga cgagggcgaa gcctacagcc gcaagctgcg
     7201 tcaggcgggt gtcgatgtca ccgccgtccg ttacgaaggt atcttccacg acttcatgat
     7261 gctcaacgcg ctggccgaga ccaacgcgac ccaggcggcg accgcgcagg ccgcactggc
     7321 gatgaaagct gcgctcgtcc cgtaaccggc aggatcgcgc catccccgcc agatatcgtc
     7381 ggcggggatg gctcatcgtc tgatgatgtt ccccgaatac gtgcaacaca cggaggcatg
     7441 gatggaaacc gaagctggaa cgctaactct tgacccgcct gtccagtcca gacagaccta
     7501 cccgtatcac actctcatca cgagcgtgcc gcacgccgag gccagggtcg tcgctgccgc
     7561 tctggtcgca gccgggttta cccgcgaccg gttcgagatc acgaccgccg aggagatttc
     7621 ctggcttggc gagcgcatcg gcgcgtcggg cgcctatggc ttgctgaacc ggctgcgttt
     7681 cagcctcggc gacgcgctgg tcgcggaagg gccggcgaga ctcggtctgc ttgaacagta
     7741 cgcactggtg cagatcctca tctacaacga tcaagagcag gctcgcgccc gaacgatcct
     7801 ggaccagtac ggcggacacc tgatgcacta cttcggccgc tggacggtca gtgacggcga
     7861 ctagcatcat cttgcggagg ggcgaaggcc gaagcctcgc cgtgcttgca acgcgccgaa
     7921 caagggtgag gctgtggcca tcgcccctcc gcgaaactcc ctcacgatgt tgctgggatg
     7981 aacgtgaacg accagttgcc atcgacctgc gacgggagct tcgggatgtc gtggttgggt
     8041 gcgaagcgga tggtgtggaa ggtgacggtc agcggctggc tggcgtcggc cggtggcggg
     8101 gcgatgacga cgtgcattct ccaggtcgta acgccggcgt agccgggctg gtggatgcgg
     8161 tggatcgcgt cgaagctggc gtcgatccga tccggtagca tcccgttcat cgtcacgtca
     8221 tcgacctgcg gcacaacgta gccggcggtc gatcggtcca gttcgatgtc gtcgagccgg
     8281 atcgagaggg tcatcgcatt cgactggagc tcgaccgatt ccagcatcag cgacagacgc
     8341 tgatcgacgg cgacaatcgg gagcgcggcc ggctcgggag gtgtcgtggc ggcgtcattc
     8401 tctgggccga agacgacgac gcccagcatc gccagcgcgc cgatggccgg ggcgatcgcc
     8461 agcaggatcg ggtggttgtt gccgctccgg cgtgcgtcgg ccgcgatcgg cgggcggagc
     8521 gtcccggcgt agatgacgag cggatcgttg gtgtgcgacc cccagtggct cgcgctgggg
     8581 acgaccgtcc gatacgggtt gtgctcgcca cggtacatgc cgagcgtcag cagcaggaca
     8641 acctcgccca tcagcaggac cagcgcaaac tcggcccagc cccaggcgcc aaacagcggc
     8701 ccgctggtcg tcgtgaccgc cagcgcaatg acaagcggca tgaccagcag caacagcgcc
     8761 agcaccagcc cgccccgtga gggtagccag tcggggaaca acgctccccg cctgccggat
     8821 ggctcctccg tcgatgatcg cgagagcatc tggcatcctc ccgttcggac aacaggtgtt
     8881 caatatctcc tgtctcttat aacgcaccgg agcacgaaaa gggacacact gatgcgggtg
     8941 ggccaaccgg tccacccgct gaaggctgtg cgctgctggc tgagcgcggc cggctagcgg
     9001 acgccctgga cgccgaggat gctggatagc gttcgcaggc gggcctgggc ggcgcggacg
     9061 ttgcggccct cggcggcggt cgtgatgatg tcggccgcga agtcgcgcag gacgtggacg
     9121 gcctccggcg tgttgaggtc gtcgtcgagc gcgccacgga agcgcgtctc gaaaccggcg
     9181 ccgtcgaact cgtcgccgtg gcgcgagtcg agccgggccg cttccttgag catgctggcc
     9241 agcccctcgt actcggccgg gccggtgtcg cggtaggacc acgcctcgcg gtagtggttg
     9301 gagtgcaggt agaggcgcag ggcatccggc gtgtggacgc gcaggacgtt gcgggccaga
     9361 acgaggttgc cgagcgactt gctcatcttc tcaccctggt agcggaccat cccgatgtgc
     9421 atccagaact ggcagaacgg ctcctcgccg ctggcgtgct gcgactgggc gatctcgcac
     9481 tcgtggtgcg ggtagatcag gtcgctgccg ccgccgtgga tgtcgatctt cggccccagg
     9541 tagcgcatcg ccatcgccga gcactcgacg tgccagccgg ggcggcccgg cccccacgga
     9601 ctctcccagg ccggctcacc cggctgctgg ccctgccaga ggatgaagtc gagcggcccc
     9661 tctttgcggg ggtcgtccgg atcggctccg cgctcacgcg agagctcgat catctcgtcc
     9721 cactcgtact tgccgagcgc gccgtagtcc tcgtcgttgg cgacgcggaa gtagacgttg
     9781 ccctggctct cgtaggcata gccgtcggcg atcagggccg ccgtgatctc gatgatcttc
     9841 tcggtctcct cgctggcacg cgggtaggcc tgcggccaga ggcagttgag ggccgagagg
     9901 tcttcgacga gttgcttggt atggcggtcg ccgagcagat cccactcttc attgtcgcgc
     9961 ttggcgcgct tgaggatgtc gtcgtcgatg tcggttacgt tctggacgta gcggacgcgc
    10021 cagccgaggt actggcagta gcggttgacg acgtcgaagg tcaggtaggt catcgcgtgg
    10081 ccgatgtggg tcgtgtcata gggcgtgacg ccgcagacat acatcttgac ggtccggcca
    10141 tcgagcggct tgaactcacg cttctcgccc gacagcgtat cgtacagttt caacacgatc
    10201 ccaccctctg ctattcgcat cccacgacga catctggcga cggcgtagta tcccacgagt
    10261 ggctgtgatg agccaatcag ccggtcctcg gcgggccgaa tgatcggttg actgtctggt
    10321 ccaaaccttg catggcgcct cccgcgccgt acgataggat cacgtgacgg ggatagacga
    10381 caggacagac ttcgaatgac agaaagcgac aataccagcg aacagcccga gggcggtaca
    10441 cgctcgcggc ggcgggggcg aggtgtgcgc ccccattggg ccgacacgcc gcagatgagg
    10501 gagaacccgt cgctgaacgc gcgcgagcgg atgggccagc caaccgagga cgagcagttc
    10561 ctggagagca agacacgcga ggaggctgcg gcagcattca tccataccga tccgtggcgc
    10621 gtcctacgga ttatgggcga gttcgtccag ggcttcgaca cgctggcgag cgtcggcaag
    10681 gccgtgacga tcttcggctc ggcgcgggtg aaggaggacg acccgatgta cgcggccgcc
    10741 cgcgagttgg cgtcccggct ggcgggcgag ggattcgcga tcatcaccgg cggcgggccg
    10801 ggcatcatgg aagcggccaa ccggggcgcg gtcgacgccg aggggcagtc gatcggctgc
    10861 aacatcgagc tgccgttcga gcagggtatg aaccagtacg tcgaagtggc cgtcaacttc
    10921 cgctacttct tcgtccgcaa gacgatgttc gtcaagtacg ccgaggcgtt cgtcatcttc
    10981 cccggcggct tcgggacgct ggatgagttg tttgaggcgc tgaccttgat ccagaccggc
    11041 aagatccaca acttcccgat cgtcctgttc ggcagcgact actggcaggg gctgcttgac
    11101 tggaccaaga acacgctgct ggccgagggc aagatctcgg cggacgacct caagctgctg
    11161 gccgtcacag actctgccga ggatgcctgc cggatcatcg tcgagtgcta cgacaaccag
    11221 tgctggctgg ccgaggatca ttcggctgcg gcgcggttag gtatcaggtg acaggagtta
    11281 ggggttggtc ctcgcgagtc agcgagccgt gttcctccca tgcgaaccag cccctaccac
    11341 gctgtcacct gtcacctgac acctaccgtg tagaatagcg accatcgcgg gctgggtggg
    11401 ggagtgggcg gttccgggac aggtgtggag gatccagttg caggttgccg gtccgggcat
    11461 tggccggttg tcgcgcgcac ggatcagcgc ggcgctggtg gttgccatcg tcctttctct
    11521 cagcctcggt acgttgccgg tccgggcggc gtccgtaccc agaagcggcc cgcatcaact
    11581 gatcgtcacg tttcgatcct cctcgcccta ctgggcgccg tatgagacgg ccgagggtct
    11641 ggccctggac gccgttgcgc gggccgatca tgggctgcgt ggccagcgcg acctggcgga
    11701 gcggcgtgac ctctcactat tgacgtacgc cgacgcggcc agtgcgagcc gcgcgatgga
    11761 gacgttgcgc cgtaacccgg atgtcctggc cgccgagccg aatgtcgagc ggcaactggc
    11821 ctgggtgccg gacgatgatg cttattccga acagtcctgg tggctggatc tgatcaacgc
    11881 gccggatggc tggaacatca acaccggcag cgacgacacg atcgtcgccg tcatcgacag
    11941 cggcgtctca ccgacgcacc cggatctctc cggccggctc gtgcccggct acaacgtcgt
    12001 tgatggcacg accgacacgt cggatatcgc cgggcacggc acgcacgtgg ccggcatcat
    12061 cgctgcccag cagaacggga tcggcaccgt cggcgtcgcg atgggcgtcc acatcatgcc
    12121 gatccgcgtt gtcgacgggg ccggcgacat cgatgtctcc aacgagatcg aagccatcta
    12181 ctgggcggtc gacaacggcg ccgatgtgat caacctcagc ctcggcgccg aggagtacgt
    12241 ccaggccgag cgtgaggcga tccagtatgc ttacaaccgg ggtgtcgtcg tcgtcgcggc
    12301 ggccggcaac tacttcaaca aggtcagcta ccccggcaac tacgatgagg tcatcgcggc
    12361 cgggtcgctc aaggcggacg gctacccagc gccgttcacc tcacgcatca cccgcgtcga
    12421 tgtctccgcg ccgggcgagc gaatctactc tcccggctgg gatgacttct tcggtgacta
    12481 ctgggacgat gtcttctacg ccgataactc gcctgtctcc ggcacgtcgt tctcatccgc
    12541 gatcgtggcc ggcgtcgtcg cgctgctcaa gtcgatcgac ccgacggccg gaaccgagca
    12601 ggtgcgcagc ctgctgaaat cgaccgcgac cgacaccggc gggatcggcg tcgccgaagc
    12661 aggcgccggg gccggtgagg tcaacgtcga ggccgccctg cgggcctcgc tggatcttga
    12721 gctgaatgag acctggcatc gcgccgacga accggtcgcc agcggcgccg tcagccggac
    12781 ctggctctgg ggccaggagg ccagcgagac gctctacgag ccgtacgacg agacccagca
    12841 cgggacgcgg ctggtcgcct actatgacaa gagccggatg gaaatcaccg acccgatgga
    12901 ggatcgcacc cagcagtggt atgtcaccaa cggcctgctg gttgtcgaga tgatcaccgg
    12961 caacctgcag cttggcgacg accacttcgt ctcgcatggc gcgtcgaacg tcaacatcgc
    13021 cggcgacccg gacgacacga gtggcccgac ctatgccgcc ttcgcgggct tgctcaacct
    13081 cccgaccgtc atgcaggatc agacgttgat cctgacggtt gaccgcaccg gccgcgtcaa
    13141 cgctaactcg cacctggcga cctacggcgt caccggtgat cagtacgtgc cagagacgaa
    13201 gcaccgcatc gctaacgtct tctgggacta cctcaacagc gtcggcccga ttgccaacgg
    13261 cagcgacctg accgatggcc agctcttcga cccctggttc tacgcgactg gctacccgat
    13321 caccgaggcg tactgggcgc gagtgaaggt gcgcggcatc atccaggatg tcctgatcca
    13381 gtgcttcgag cggcgctgcc tgacctacac gccggccaac gcggagggct ggaaggtgga
    13441 gatgggcaac gtcggcctgc actactacga ctggcgctac aacagttccg caacgctcga
    13501 cccgaacctc acgatccgcg ttccggccac cgccaacccc ggaccgcgcc aatccctgcg
    13561 ccaactctgg cgcactggct tcaactagcc cacgccgcag gacacgataa ggacgtttca
    13621 cgtaccccct ggtcccctat gctggtgact ggaaaccacg acctgcgaga agtagatgct
    13681 caaaacccgt gtgtgtgagg tgagcatcta cttcctgctc cgtctcttgc cgtgaggaga
    13741 agaggactgg ttgcctgtga gagtggaata tcttcgtaga gagccttttc ctcatgggaa
    13801 acggtcagaa tctctctcat gacactcacg ggactctccg gtagggtgtt gctcgttgca
    13861 gtagaagtag atgctcacgt cacacacacg ggttttgagc atctactccc agcacaggtg
    13921 cgttcgcatc caacctgaag gatcgtcctc caattttcct atggaacaga aagcatgcag
    13981 ccgctatcct tgagcgatta tcctcggggt gaggaatgag actcatgcgg ctgccgggcg
    14041 gacgggctgc ggcggctgcc tgactggggg aattctctgt gacccatgtt ctgcgccatc
    14101 cgatggcggc ggttgtcgtg ctggcgctgt tgctatccac cgtcacacca gtcctcgcga
    14161 ttggatcgaa cccaccggag ccggacggac ctggatcggg cgccgagttg ctcgccggtg
    14221 ctgagaagct caagcacccg acacttgatt ccagcctctt caagatcgcc agggacgttc
    14281 gcgccggcaa gcagccgaaa gacgcgatcg cccgtcaggc gaaggtatcg cggggcgaga
    14341 gcgtcgccgt ctcggtcgat gtcgccggtg atccggcggc gaccgtcgcc tggctcaaca
    14401 gccacggcgc gcaggtcgac aacgtcggtc agcggctgat cgaagcctat gtgccggtgg
    14461 cggaactgga gtcattgggg agcctgtccg gcgtcctggc cgtgcgctcg atgaggcctc
    14521 catttgccag cgttatgtcg cagggcgtgg cgttgcatgg tgtcccgaac tggaacacca
    14581 acggctacac cggcgccggc gtcaaggtcg gcgtcatcga cgttggcttc gacggcatcg
    14641 tctcgctggt cggtagcgag ctgccgcaac tgactgcgcg ctgctacccg acgtccgggg
    14701 cggtcacctc gaacgcccag gattgcgacc ggaacggtgt ccacggcgcg gccgtcgccg
    14761 agacggtgat cgacgttgcg ccgggagcgt cgctgtatat ctccaacccg cgttcgtggc
    14821 tggaactgaa tgccaccgtc aactggatga tctcccaggg cgtgaccgtc atcaaccact
    14881 cggtcggctg gggctgggaa ggccccggcg acgggacgac gatctacgcc gacagcccgc
    14941 tcggcgccgt tgatcgggcc gtcgccggcg gaatcacctg ggtcaacgcg gccggcaacg
    15001 agggcctcgc gacctggtcg ggcgactatc tcgattccaa caacgacaac atcatggagt
    15061 tcgcgtccgg tggggtcgat ctcaactcgg tcccggccta ttcgggccag tcgatcgacc
    15121 tgatgatgcg ctgggacgat agctggccga gggcgacctc cgatctcgac ctgtatctgc
    15181 tcgatagctt cggcaacatc gtcgcgacca gccaggagca gcagaacggc ggctccggcg
    15241 acacgccgat tgagatcctg tcgtacatgc cgagcgattc gggctacctc tatatcgcgg
    15301 tcgagcagtt cggcggggcc gatccggatt gggtccagat ccaggagttc aacggactgc
    15361 cgctgggcct ctcgtcacct gagtacagca tcgccaaccc ggccgagagt gccaaccccg
    15421 gcctgctggc ggttggcgcg gcctcgtggt tctcgcccag cacaatcgag gatttctcca
    15481 gccaggggcc gacccgcgac ggccgcatca agccggatgt cgtcgggatc gaccaggccg
    15541 acacggtctc ctacgggccg tccggcttcc ccggcacgag ccaggcgtca ccgcacgtcg
    15601 ccgggctggc cgtgctggtc aagcagcgct tcccgcagta cagcgcggcc gaggtcgccc
    15661 agtacctgcg ctcgaacgca tcgcaggcgc aggcgccgaa caacgtctgg ggctacggcc
    15721 tcgcctacct gccggagatc gtggctggtg agaacccggc cccggccatc agcagcatct
    15781 cgcccgcaac cgtccagctc ggcagctatc cactgacgat caccgtcaac ggcaacggct
    15841 tcgtgccgga gtcggttgtt cattggaatg gcgaagatcg ggcgacaacc tacgtcagcc
    15901 caagccagct cacgtttgag gtcgactacc gcgacaccga cgttgcctcg acggtcgaga
    15961 tcacggtcag tagccccggt ccgggcggcg gtgtctcgaa cgctgtgacg ctgacgatcg
    16021 acgatgacac gccacaggtg ccgaacgccg tcgactatcc cgagttcctg cgaacctggc
    16081 agcggacgga cgagccggtc aagcaactgg tcgcgcagcg tacctggatg tggggcgatg
    16141 gccaggccag cgatgcgatg gtcgaggcgt acgcggatgc ccccggcggt taccgctcgg
    16201 tcaagtactt cgacaagagc cggatggaag tcaacgaccc gaacgaggac aacacccagt
    16261 cgtggtacgt caccaacggc ctgctctcga aggagctgat caccggccag atgcagatgg
    16321 gcgacgacgc gttcgttgac cacggcccgg ccgaggtgcc ggtcgccggc gacccgaacg
    16381 acgccaacgg cccgacctac gcgaccttca ccgacctgct cgacgcggcc ccggcccaga
    16441 gcggatcgat gctcacccag cgcatcgacc gcaacgggac cgtcacggac gacccgtcgc
    16501 tggcgcagta cggcgtgacg gccgcctcac tggttgaggt gcccggcatc cgccaccagg
    16561 tcgcctcgcc gttctgggag ttcatgaact cgaccggcac gatctacgcg aacggccagt
    16621 acctgacgtc ccagctcttc gacccgtggt tctacgcgac cggcctgccg atcaccgagg
    16681 cctactgggc aacggtcaag gtcaacaacc agtacgtcga tgtcctgatg cagtgcttcg
    16741 aacgccgctg cctgacctac acgcccggta accccgacgg ctggaaagtc gaagccggca
    16801 acgtcggcct gcactactac gtctggcgct acgggatgat gccgtaggga cgggaatcgg
    16861 gactcgggat tcggggctgt caaacggcac ttctccgttc aacgcccccg aatcccgagt
    16921 cccgattccc gtccctacgg catcatcccg taacgccaga cgtagtagtg caggccgacg
    16981 ttgccggctt cgaccttcca gccgtcgggg ttgccgggcg tgtaggtcag acagcggcgt
    17041 tcgaagcact gcatcaggac atcgacgtac tggttgttga ccttgaccgt tgcccagtag
    17101 gcctcggtga tcggcagg
//
LOCUS       DASUGJ010000414         7794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_481447, whole genome shotgun sequence.
ACCESSION   DASUGJ010000414 DASUGJ010000000
VERSION     DASUGJ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481447"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFV93_09085"
     CDS             complement(<1..183)
                     /locus_tag="VFV93_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX5165536.1"
                     /translation="MRKTTLYIPDDLKRELERVAELEGRSEAAIIREGIQLAINKCTP
                     PSPTLWTFTADDPDFID"
     gene            291..1412
                     /locus_tag="VFV93_09090"
     CDS             291..1412
                     /locus_tag="VFV93_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX5165537.1"
                     /translation="MPARTHHIQIDPWTPLGQQESVGHNRWHEDIPPVLEVEQGDRVV
                     IDTMDAFDTQVAREMSHAGAGQLDLGPVHPLTGPVYVKGAEPGDLLEVRLLDIEADPW
                     GNHGYTAEVPGFGFLRDIFTEPYLVHWDISGDYAESPELPGVRLRYGAHPGVLGLAPS
                     KELRERILAREGALLERGGAVLMPMADGALPANLGNEALRTVPPREVGGNIDIKQITP
                     GANVLMPVYVDGALFSTGDVHFAMGDCEACGTGVEMRSRVHVSFKVHKGEAARRNIRA
                     LQFYRDTYFNAPELAAPKRFYATTGYSVTEDDENRSEDLTLAARNALLAMIDYLGSRG
                     FDRQQAYALCSVAVDLRVSQVVDVPNFIVSALLPLDIFL"
     gene            complement(1501..1722)
                     /locus_tag="VFV93_09095"
     CDS             complement(1501..1722)
                     /locus_tag="VFV93_09095"
                     /inference="COORDINATES: protein motif:HMM:NF018554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEX5165538.1"
                     /translation="MAQPNETFLVWLALQARRDDAVGDCARDMLDDPDWPADATTFEP
                     ARAYLESVHASPKAIEALREAWDEWRAKS"
     gene            complement(1761..2096)
                     /locus_tag="VFV93_09100"
     CDS             complement(1761..2096)
                     /locus_tag="VFV93_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165539.1"
                     /translation="MTDPATRRQREADADPMRDAHADFHVSEEARRHNDPTKQVYGAY
                     DDADEAIREARPPRGDDETIFELEGDVVSSDAVPTQPSHDGIGPFATPVIIVVVALAL
                     FIILLVVLT"
     gene            complement(2299..3060)
                     /locus_tag="VFV93_09105"
     CDS             complement(2299..3060)
                     /locus_tag="VFV93_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165540.1"
                     /translation="MTEQSATEPQMQAPPPRKNGGARRWLAGGLVGVALGALLLGGTA
                     FAQTDSSGTDLRQTFIDRLASKLGIASDDLETAITDTQVEMIDEALADGRMTQRQADV
                     LRERAASGDGLFRMRPEFGMRAMHALDINLATIASELGMTTEELRAELQDGSTLSEII
                     IAHSSTVDAVVEALVANAETQLNEAVANGNLTQAQADQILTNLPDRLTQMIENGFPGL
                     CGPWFNDGQEQHQDGTSTPESSDDTDNADETSNQV"
     gene            complement(3178..3516)
                     /locus_tag="VFV93_09110"
     CDS             complement(3178..3516)
                     /locus_tag="VFV93_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165541.1"
                     /translation="MSAMPTLVVTEESAVRTTLGTVWQVDHQVRSADEGDIDHFSNAS
                     QTSDDWLHLNSSERVNLRYVASAWLSFGTIPNPDYRATLTGFDGLTISLRGERVVCLQ
                     DELLKRVRRG"
     gene            complement(3643..3855)
                     /locus_tag="VFV93_09115"
     CDS             complement(3643..3855)
                     /locus_tag="VFV93_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165542.1"
                     /translation="MTLPQLPAADWPAPLALDYAIDRVVREVLGSGRSVQEAITRVTE
                     LAEALGPQLTQAEIRKRVHAQMIANS"
     gene            complement(4376..5170)
                     /locus_tag="VFV93_09120"
     CDS             complement(4376..5170)
                     /locus_tag="VFV93_09120"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX5165543.1"
                     /translation="MEAGVYLPKLRWRTASCRTWRTLPFWWEDQVKVVGVDGCRGGWI
                     AIVWDTSAATVTPRFHATFSEIVDSYPDAACIGIDIPIGLRDDGLPRSCDVDARQLLG
                     WPRMASVFPAPQRQLLACANYSAATTLSRERFGRGVSRQAFGIFGKVAEVDQLITPAL
                     QRRIVEVHPELSLWTMNGRQAMGYSKKLPEGFDERREQLAHHVHAEIPESCVAARQLA
                     PFAAADDLLDAMTVAWTARRAATQAANRIPAQPELDARGLRMEIVY"
     gene            5323..6156
                     /locus_tag="VFV93_09125"
     CDS             5323..6156
                     /locus_tag="VFV93_09125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5165544.1"
                     /translation="MRFGVQIRATAQTVDLRELGRALEANGFDALYLPEHTHIPLSVR
                     SLFPDDPTWLEACKQMVDPFVALATVAAVTERLLLGTGVCLLPQHDPIVLAKTVATLD
                     LVSNGRVLLGIGAGWNAPEMTNHGVDPRQRWAVMREHALAIRAIWTSDVAEFHGRFVD
                     FDPIWLWPKPVQQPHPPLVIGGEGPHVLDRVLDYGDEWMPNEHEGIEDRITELQRRAA
                     DEGREPIPVTIYATPTDPATIERLSASGAHRLVFNLPQTAPGDEMRGVHHLAALIRTY
                     L"
     gene            complement(6161..7690)
                     /locus_tag="VFV93_09130"
     CDS             complement(6161..7690)
                     /locus_tag="VFV93_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5165545.1"
                     /translation="MAGLPRQIDVLVVGGGTSGAALAGIIARDTTKSVVLLEAGPDFG
                     PLDGGRWPADLLDARRFALSHDWGYDGINHASQPDRCAFPRARVIGGCGAHNGCVAVA
                     GHRLDYDGWAAAGNPGWDWASVAPAFARAREALRVRVPDASELNPFQASFLDASVAAG
                     FPRVENLDVPDLVNGVAPAPFNIHNGTRWSSALGYLDPVRGNANLTVVGGALVDRVVI
                     NGGQAVAVEATIDSERTRIEAGTIVLSGGAYGSPAILLRSGVGDPAQLRDLGVDVVHE
                     LAGVGKMLTDHPISSVQIEPSERLLNEMNAFKETGWLPDEQVVLLASSRHARQAAFDL
                     HFPAYSSQDPATGEWRFQISVGQFDTASYTGTLTLTSLDPAAPPAIDHGYLSDTAGRD
                     RDTLVHGLEIGLDIVGKMVDNGYVATILAPDPTRLGRDDLIRHLEMSVRTCYHPGCSC
                     RMGPTGDPTAVVDTTGAVHGLRGLHVCDASIFPILPRANNNLPAAMLAEMLAPVIGRN
                     A"
BASE COUNT         1255 a         2564 c         2531 g         1444 t
ORIGIN      
        1 atcgatgaag tcgggatcat cggcggtgaa cgtccatagt gtcggggatg gcggcgtgca
       61 cttgttgatg gccagttgaa tcccctcgcg gatgatcgct gcctccgagc gaccctccag
      121 ttcggcaact cgctccagtt cgcgcttcaa gtcatctggg atgtacagcg ttgtctttct
      181 cataccatac atactaccac acaacccagc aaatccacgt gcgcggtctt cgctaacgta
      241 cgctacactg cccgcaaccg actactgcac attggcgaag ggagctggtc atgcctgcca
      301 ggacgcatca tatccagatc gacccgtgga cgccgctggg acagcaggag agtgttggcc
      361 acaaccgctg gcacgaggac attccgcccg tgctggaggt tgagcagggt gatcgcgtcg
      421 tcatcgacac gatggacgcc tttgacaccc aggtagcccg cgagatgtcg catgcgggcg
      481 ccggccagct cgatctcggc cccgttcatc cgctgacagg cccggtctac gtcaagggcg
      541 ccgagcccgg cgatttgctg gaggtccggc tgctcgacat cgaggccgac ccgtggggca
      601 atcacggcta cacggccgag gtgccgggct tcggcttcct gcgcgacatc ttcaccgagc
      661 catacctcgt tcattgggac atcagcggtg actacgccga atccccggaa ctgcccggcg
      721 ttcgcctgcg ctacggggca catcccggtg tgctcgggct ggcgccgtcg aaggagctgc
      781 gcgagcggat tctcgctcgt gagggtgcct tgctggagcg gggtggcgct gtcctgatgc
      841 cgatggccga cggcgcgctg ccggccaacc tcggcaacga ggcccttcgg actgtcccgc
      901 cgcgcgaggt cggtggcaac atcgatatca agcagatcac gcccggtgcg aacgtgctga
      961 tgcccgtcta cgttgatggt gcgctgttct cgactggcga cgtccacttc gcgatgggtg
     1021 actgcgaagc ctgtgggacc ggcgtcgaga tgcgtagccg ggttcatgtc tcgttcaagg
     1081 tccacaaggg cgaggcggcc cgacgcaaca tccgcgcgct gcagttctac cgcgatacct
     1141 atttcaacgc gccggagctg gctgccccga agcgcttcta cgcgacgacc ggctactcgg
     1201 tgactgagga cgacgagaac cgctccgagg atctgacgct ggcggcgcgc aacgcgttgc
     1261 tggcgatgat cgactacctc ggctcgcgcg gcttcgaccg ccagcaggcc tacgcgctct
     1321 gctcggtcgc cgtcgacctg cgcgtctcgc aggtcgtcga tgtccccaac ttcatcgtca
     1381 gcgccctgct gccgctggat atcttcttat gaggcattga gaccccgctc cgcactcgtc
     1441 acattcgtag ggacagatct cagatcggtc tgcggccgga ttgaccattg attgagggga
     1501 tcagcttttg gcgcgccatt cgtcccacgc ctcgcgcaga gcctcgatgg ctttcgggct
     1561 ggcgtggaca gattcgagat aggcgcgggc cggctcgaac gtcgttgcgt cggccggcca
     1621 gtcgggatcg tcgagcatgt cgcgggcgca gtcgccgacc gcgtcgtcgc ggcgtgcctg
     1681 aagggccagc cagaccagga acgtctcgtt gggttgcgcc atcagtcagc acctcgtcgt
     1741 ggatccatgc gctgtggcga tcaggtgagg acgacgagca ggatgatgaa gagcgccagc
     1801 gcgacgacga cgatgataac cggcgtcgcg aatggtccga ttccgtcatg cgatggttgc
     1861 gtcgggacgg catcggacga gacgacatcc ccctcaagct cgaagatcgt ctcgtcgtcg
     1921 ccgcgtggcg ggcgagcctc gcggatcgcc tcatccgcgt cgtcgtatgc cccgtagacc
     1981 tgtttcgtcg gatcgttatg gcgtcgcgct tcctcgctga cgtggaagtc agcgtgcgcg
     2041 tcacgcatcg gatcggcgtc ggcctctcgt tggcgacgtg tggctggatc agtcattgga
     2101 acccctgcaa tcttcggtct gccgcgctac ggaagacact ggtggtaacg ccggcgtgcc
     2161 tggctccgct gcgtgcaagt tccatgccga tcatgatggc agcgcgaacg acattcaggc
     2221 tgttcgaggg aagagaaacc ggccccgttt caggggccgg tttcgtcgcg atcttgccct
     2281 cggcgccgtt gccgggaatc agacctggtt cgaggtttcg tcggcgttgt cggtgtcgtc
     2341 gctgctctcc ggcgtgctgg tgccgtcctg gtgttgctcc tgcccgtcgt tgaaccacgg
     2401 gccgcagagg ccggggaagc cgttctcgat catctgcgtt agccggtcgg gcaagttggt
     2461 gaggatctgg tcggcctgcg cctgtgtcag gttgccgttg gccacggcct cgttcagttg
     2521 cgtctcggcg ttggcaacca gcgcctcgac gacggcgtcg acggtcgagc tatgggcgat
     2581 gatgatctcg ctcagcgtcg agccgtcttg tagctcagcg cgcagctcct cggtcgtcat
     2641 cccaagctca gaggcgatcg tcgccaggtt gatgtcgagg gcgtgcatgg cacgcatccc
     2701 gaactccggc cgcattcgga agagtccgtc accggacgcc gcccgctcgc ggagcacgtc
     2761 ggcctgccgc tgcgtcatcc ggccgtcggc gagggcctcg tcgatcattt ccacctgcgt
     2821 gtcggtgatg gccgtctcca gatcgtcgga ggcgatcccc agcttgctgg ccagccggtc
     2881 gatgaacgtc tggcgcaggt cggtaccgct gctgtcggtc tgggcgaagg cggtgccgcc
     2941 gagcagcagc gcgcccagtg cgaccccaac cagcccaccg gccagccagc gtcgggctcc
     3001 gccgttcttc ctgggtggtg gcgcctgcat ctgcggctct gttgccgatt gttcggtcat
     3061 cgcgtgcgtc ctttcatagc gtcgggtcga gcgaccatca ctggttcgac tctaccgact
     3121 gatcctgaaa ggaggctggg agatagattg gagttctcta aacgtggagt tggttgatca
     3181 gccgcggcgg acgcgcttga gcagctcatc ctggaggcag acgacccgtt cgcctcgcag
     3241 cgagatcgtc aggccgtcga agccggtcag cgtcgcgcga tagtcggggt tcgggatcgt
     3301 accgaacgac agccaggcgc tggcgacgta gcgcaggttg acgcgctcgc tgctgttgag
     3361 atgcagccag tcgtcgctgg tctgacttgc gttggagaag tgatcgatgt cgccctcgtc
     3421 ggcgctgcga acctgatggt ctacttgcca gactgtgccg agcgttgtgc gcacggctga
     3481 ctcttcggtg acgaccaagg ttggcatggc gctcattgcg agggctccta cgtagcgtcg
     3541 atcgacgttc ccaccgcagt atcgtcccgc gccatcgccc gtgtcatcac ctattcgtca
     3601 ggatttctcc cggtccggat gtcccgtcgc tgctgccgtt cgtcagctgt tggcaatcat
     3661 ctgggcatgg acccgcttgc ggatctctgc ctgggtcagc tgcgggccaa gcgcctcggc
     3721 cagttccgta acgcgggtga tcgcctcctg gaccgagcgg ccagagccga gcacctcacg
     3781 gacaacccgg tcgatcgcat agtccagcgc gagcggggcc ggccagtcgg ctgcgggcag
     3841 ctgcgggagt gtcatctcgt tccacggtgc gccgggcgac acctgtcggt tggctgccgg
     3901 ctgtctatcg gctggtccgg tcgtccattc atccgtcagt cgtttcatag gtcgagtgta
     3961 cgtacgtctc cggcagcggt catctcccgt tgttcccaat cggcgcgtgc cgttcgtagc
     4021 ccgaactggc tatgcgggct ggtacctgcc gcgaaagccg tccttttgga caggtcgtgc
     4081 gttgtccgtc gtgtcgatgt gccgcgagcg gccaccacct atcatgatga cattgaaggt
     4141 tgacaacgac gtcaacccgg acgcgaagca gcgtcacgag ctcgaagacg agagggatgg
     4201 cggatgtacc ccgatatgct cattgccgag ctgaaccacc gcgtggaaga gctttcacgc
     4261 agccggagtc agtcgaagat cgctcgcctg ctgcgtcgct agccagcgag cgattcacca
     4321 cccacgaaac gcgcctgtca tctcggcagg cgcgtcgtcg ttgttggctc aagcgtcagt
     4381 agacgatctc catccgcaac ccgcgcgcgt cgagttccgg ctgggccggt atccggttcg
     4441 ccgcctgcgt tgccgcccgt cgcgctgtcc aggcgacggt catcgcgtca agcagatcgt
     4501 cggccgccgc gaacggcgcc agttgcctcg ctgcgacgca gctttccggt atctcggcat
     4561 gcacgtgatg cgcaagctgc tcccggcgct cgtcgaaacc ctccggcagc ttcttggaat
     4621 accccatcgc ctgccggcca ttcatcgtcc acaacgacag ttccggatgc acctcgacga
     4681 tccggcgctg caatgcaggc gttatcaact gatcgacttc ggcgaccttg ccaaagatcc
     4741 cgaatgcctg tcgcgacaca cctctgccaa atcgctcgcg tgagagtgtc gtcgcagccg
     4801 aatagttcgc gcacgccagt aactgtcgct gcggagcagg aaagacgctc gccatccggg
     4861 gccaaccgag gagctgccgg gcgtcaacat cgcaacttct gggcagcccg tcgtcccgca
     4921 acccgatcgg gatgtcgatt ccgatgcagg cggcgtcagg gtagctgtcg acgatctcgg
     4981 agaacgttgc gtgaaagcga ggcgtcactg tcgcagcgct cgtatcccag acaatggcga
     5041 tccacccacc gcgacaacca tcgaccccaa cgaccttcac ctggtcctcc caccagaatg
     5101 gtaacgtacg ccatgttcga cacgaagccg tccgccaccg caatttcggc aggtaaacac
     5161 ccgcttccat attgtcatcc gaggagagat ccttcggctc acgcctcagg aagacaaaga
     5221 gagaggcgtc ctgtctcccg catcacggcc agcgttggac ggcgtcgccg tccaaccgat
     5281 cgttcgttct ggcagggatc gacgctcaca gaggaggctc cattgcgatt cggtgtccag
     5341 attcgggcga cggcgcagac ggtcgatctc cgcgagcttg gccgggcgct cgaggcgaac
     5401 ggcttcgatg cactctacct accagagcac acgcacatcc cgctctcggt ccgctcgctg
     5461 ttccccgacg atcccacctg gctggaagcc tgcaagcaga tggtcgaccc gttcgtcgcc
     5521 ctggcgaccg tcgcagccgt caccgagcgg ctgctgcttg gaaccggtgt ctgcctgctg
     5581 ccgcagcacg acccgattgt gctggccaag acggtcgcga cgctggacct tgtctcgaac
     5641 gggcgcgtcc tgctggggat cggcgccggc tggaacgcgc cggagatgac caaccacggc
     5701 gtcgacccgc gccagcgctg ggcagtcatg cgcgagcatg cattggcgat ccgcgcgatc
     5761 tggaccagcg acgttgctga gttccacggc cgcttcgtcg acttcgaccc gatctggctc
     5821 tggccgaagc cggtccagca accccaccca ccgctcgtca tcggcggcga gggtccgcac
     5881 gttctcgacc gtgttctcga ctacggcgac gagtggatgc cgaacgagca tgagggcatt
     5941 gaagaccgca tcaccgaact gcaacgccgc gccgctgatg aaggccgcga gccaatcccc
     6001 gtcactatct acgcaacccc gaccgaccct gccaccatcg aacgcctctc cgcctcgggc
     6061 gcgcaccgcc tggtcttcaa cctgccccag accgcccccg gcgacgaaat gagaggtgtt
     6121 caccacctgg cggcgttgat acgcacgtat ctgtgatgcg tcacgcgttt cgcccaatca
     6181 ccggcgccag catctcggcc agcatcgcgg ccgggaggtt gttgttggcg cgaggcagga
     6241 tcgggaagat cgaggcgtcg cagacgtgca ggccgcgcag gccgtggacg gcgccggtcg
     6301 tgtcgacgac ggccgttggg tcgccggtcg gtcccatccg gcagctacag cccgggtggt
     6361 agcaggtgcg caccgacatt tcgaggtggc ggatcaggtc gtcgcggccg aggcgagttg
     6421 ggtccggcgc gaggatcgtg gcgacatacc cgttgtccac catcttgccg acgatgtcga
     6481 gcccgatttc cagtccgtgg acgagcgtat cgcggtcccg gccggccgtg tcggacaggt
     6541 agccgtggtc gatcgctggt ggcgcggccg ggtcgagcga ggtcaacgtc agcgtgccgg
     6601 tgtaggaggc cgtgtcgaac tggccgaccg agatctggaa gcgccattcg ccggtcgctg
     6661 ggtcctgcga cgagtaggcc gggaagtgca ggtcgaacgc cgcctgccgc gcgtggcggc
     6721 tggaggccag caggacgacc tgctcgtccg gtagccagcc cgtttccttg aatgcgttca
     6781 tttcgttcag caatcgctcg ctgggctcga tctggacgct ggagatcgga tggtcggtca
     6841 gcatcttccc gacgccggcc agttcgtgta cgacatcgac gcccagatcg cgcaactgag
     6901 ccgggtcacc gacgcccgag cgcagcagga tcgccggtga gccgtaggcg ccgccgctga
     6961 ggacgatcgt cccggcctcg attctcgttc gctcgctatc gatcgtggcc tcgacggcaa
     7021 ctgcctgccc gccgttgatg acgacgcgat cgaccagcgc accgccaacg acggtcaggt
     7081 tagcgttgcc gcgcaccgga tcgaggtagc ccagcgccga cgaccagcgc gtgccgttgt
     7141 ggatgttgaa tggcgccggt gcgacgccgt tgacgagatc cggcacgtcg agattctcga
     7201 cgcgggggaa gccggctgcg accgatgcgt ccaggaatga cgcctgaaac gggttgagtt
     7261 ccgacgcatc cggcacgcgg actcgcagcg cctccctggc acgtgcgaag gccggtgcga
     7321 cgctggccca gtcccagccg gggttgccgg cggctgccca gccgtcgtag tcgagccggt
     7381 gaccggcgac agcgacgcag ccgttgtgcg ccccgcagcc gccgatgacc cgggcgcgtg
     7441 ggaaggcgca gcgatccggc tggctggcgt ggttgatacc gtcgtagccc cagtcgtgtg
     7501 agagggcgaa gcggcgcgcg tcgagcaggt cagccggcca gcgaccgccg tcgagcggcc
     7561 cgaagtccgg ccccgcctcc agcaggacaa ccgacttcgt cgtgtcccgc gcaatgatcc
     7621 cggccagcgc ggctccggat gtcccgccgc caaccaccag cacgtcaatc tgcctcggca
     7681 atccggccat ctgcccctcc ctcgctgctg ttgacacgtt ggatggcatc gtagcacggg
     7741 gcggcatcgg gattggccga aatcggctcc ccctgtcgtc ctgagccgca gcga
//
LOCUS       DASUGJ010000415         6090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_203556, whole genome shotgun sequence.
ACCESSION   DASUGJ010000415 DASUGJ010000000
VERSION     DASUGJ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203556"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFV93_09135"
     CDS             complement(<1..807)
                     /locus_tag="VFV93_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165546.1"
                     /translation="MAQKSILQRLVRGFSVMAVLALLGMMLLACGEDDATTSNSGSGG
                     EVTPTAQPSASPTDEPASPTTNPDTIDHPTGDDDVIIRIEDVGGFVMQQSLITRLPSF
                     ILFGNGCYVVQGPQIEIYPAPALPNLQETCLSEEGVQQILAKAKAAGLLDGDAEYGNN
                     MIADATTTVFTVTAGGKTSVVRAYALGMDTDANDPNMSDEQREAREKLVSFMNDVAGV
                     ETWIEPEMIVEEAHPYEIERMQIAVLPADSTSAPQLDAGMEPQTLEWPLAT"
     gene            1113..1451
                     /locus_tag="VFV93_09140"
     CDS             1113..1451
                     /locus_tag="VFV93_09140"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5165547.1"
                     /translation="MPQYLISFDDGTMIIPDGELPAVSDAAHAVVQEARDAGVWVFGG
                     GILSQRASIVAPDGTVSAGPYPETKAVLGGFSIVDVPSREDALAWAAKFARACRCAQE
                     VREIGLDEDA"
     gene            complement(1552..3291)
                     /locus_tag="VFV93_09145"
     CDS             complement(1552..3291)
                     /locus_tag="VFV93_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165548.1"
                     /translation="MTNHPDIDNELDELGQAVRRAFAGESLSAEQQQRHLGRLRGRRK
                     GARAGMLDAGRRPLESRSLGRLRLIPRDGGGQGRTHPGLQFVAGIAAVAVVAVALVAL
                     FEGLAGLRDADRQAAGTIETPNPRGWTYPGADLPGGGQLAFVSSQDGVPQIAVVNADG
                     SGLASVTTDASTHWSPGWSPDGEQLTWSSDSRGAAQVYVANADGSGVVQRSIFLDNRD
                     PLWSPDGAWIAFISTRDMRTDIYVMPAAGGREVSITNGAAVDWGANMVWSPDSSALAF
                     ETAGDLYIVNRDGSGLRRLTSDPAIDSAPDWSPDGSRIAFFSNRDDNFDIYVINTDGS
                     GLINLTETPEDEYQPVWSPDGWTIAYLRAPVTSEADHETQLWSMGANGAGVHRLGDIS
                     GYALSAPSWSPDSRQLAVIAAPEGSDLSAGTIDDGELYVIGAGGSDQRLIVDHAADLG
                     RPLWRPLWWPQTTATGSTGASQPALTLSPAQGNCASQITVRGSGFEPGAVVNLYGAPA
                     IDSNFAPVAQGVIVAADGSFETPIDLRRFAGCSGQTPTGGVFNIGATIERGLRNGAML
                     SDLSASADFTVRE"
     gene            complement(3418..4023)
                     /locus_tag="VFV93_09150"
     CDS             complement(3418..4023)
                     /locus_tag="VFV93_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5165549.1"
                     /translation="MVSKGTVFGQVGGRAVVPTASLPLDSDEALAVAARTDAECFSQL
                     YLRYSNRIYRYAISRTRSATAADDIVSDTMMSALEGINGFNPDRGSFASWIFTIASRR
                     IADRDRKSRQFWRYLARRGHHEEHDEDALTTTLRLERRDDVRQAISRLTDQHQQVVLL
                     RYVAELPIATIGEILDISEGAVKMRLQRALKRLAVELGGDE"
     gene            4263..5828
                     /locus_tag="VFV93_09155"
     CDS             4263..5828
                     /locus_tag="VFV93_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5165550.1"
                     /translation="MQQPQYDAVIVGGGHNGLVASFYLARAGMKTLVLERRDFVGGAA
                     ITEELFPGYSVPSCSYICYLLQPRIIDDMDLREYGFEILPAAKDTFMPFPNGRSLLDG
                     REGRSIEEQIAEFAPGDAVNLPKYREIRRRMAGIIHQSFFEKAPTLTELVDSVRGTED
                     EPLLERLLFGSVADLLDEHFEDEQIKGWLAQAWDAGDIDAPGSLLSAAFLGVSAFTRD
                     GDYGLVHGGMGTITQSMARSCQAQGVEIRTNARVEQLLVEDGRAAGVRLESGEEIRSR
                     LVLVNADIKQLFNKLVPKGAVSDDFVRAVNRLKSNTLYYKFHAALNELPDFSRWLGSD
                     PDPYYLGYVKICPSMDYYRKAWNDARNGLITDSPVMHIQMASVIDRSLVPGGGHVMSI
                     WALYAPHKPTGHGSEVWTPELTRLAGETFIDRMEEYAPNIRSAIRDWVMFTPNILEER
                     VGLPNGHIHHIDQIAGQMFANRPLPGWADYQMPLDGLYLCGADTHPGGEVTGANGYNA
                     AHKVLEDARVLVG"
BASE COUNT          973 a         2025 c         2078 g         1014 t
ORIGIN      
        1 ggtcgctagt ggccattcga gcgtctgcgg ctccatgccc gcgtccagtt gcggcgcgct
       61 ggtggagtcg gccggcagga cggcgatctg catgcgctcg atctcgtatg ggtgggcttc
      121 ctcgacgatc atctccggct cgatccaggt ctcgacaccg gcgacgtcgt tcatgaagct
      181 gaccagcttc tcacgcgctt cgcgctgctc gtcggacatg ttcgggtcgt tcgcgtcagt
      241 gtccatcccg agcgcatagg cgcggaccac cgaggtcttg ccgccggccg tgacggtgaa
      301 gacagtcgtc gtcgcgtcgg cgatcatgtt gttgccgtac tcagcgtcac catccagcag
      361 gccggccgcc ttcgccttgg ccagaatctg ctggacgccc tcttcactca ggcaggtctc
      421 ctgcaggttc ggcagggccg gggccgggta gatctcgatc tgcggtccct ggacgacgta
      481 gcagccgttg ccaaacagga tgaagctcgg caggcgggtg atcagcgact gctgcatcac
      541 gaagccgccg acatcctcga tccggatgat cacatcgtcg tcgccggtcg ggtggtcgat
      601 cgtgtcaggg ttggtcgtcg ggctggccgg ctcgtcggtc ggcgacgcgc tcggctgggc
      661 ggtcggggtg acctcgccgc cgctgccgct attgctggtc gtagcatcgt cctcgccaca
      721 ggcaagcaac atcatcccca gcagagccag gaccgccatc accgagaacc cgcggaccag
      781 tcgttgcaga atcgacttct gcgccatcgt cgtagctcca tcttcctcag atgccatcgg
      841 ggcgagattc ccgctcgccc gagagactcc gttgtctctg catctgagac gccacggcgt
      901 tccagttggt tcccgcgcca tgcaccaatc ttcagaaact gtccgactgg tcaggctcct
      961 ctctaaacgg gcagccggta cgatcattcg atcatcccga ggcaccgtca ggaacgctgg
     1021 atcccggcca gatccttgac aacccgtcat ccgaacccgt agaaggtgta cgtacactgt
     1081 tggtacagag cgccaggaca aggagtcgtg aggtgccgca atacctgatc tcgttcgatg
     1141 acggcacgat gatcatcccc gatggggaac tgcccgccgt gtccgacgcc gcgcacgcgg
     1201 tggtccagga ggccagggat gccggcgtct gggtgttcgg tggcggcatc ctcagtcagc
     1261 gcgcgagcat cgttgccccc gacggaacgg tcagcgccgg cccgtacccg gaaaccaagg
     1321 cggtcctcgg cgggttctcg atcgtcgacg tgccctcacg cgaggatgcg ctggcgtggg
     1381 ctgcgaagtt cgcccgggcc tgccggtgcg cgcaggaggt ccgcgagatc ggactcgacg
     1441 aggacgcctg attgccggca tctcgctccc acaccgtgcc cacgggccgc aaccacggcc
     1501 ctgaagcatt gatcagctgg agcgccgctc agcgagcgtc tcccgggttg attactcgcg
     1561 gacggtgaag tcggcgctgg cggagaggtc ggagagcatg gcgccgttgc ggaggccgcg
     1621 ttcgatggtg gcgccgatgt tgaagacgcc gccggttggg gtctggccgc tgcagccggc
     1681 gaagcggcgc aggtcgatcg gcgtctcgaa gctgccgtcg gcggcgacga tgacgccctg
     1741 ggcaaccggc gcgaagttgc tgtcgatggc cggcgcgccg tagaggttga cgacggcgcc
     1801 cggctcgaag ccgctaccac ggacggtgat ctggctggcg cagttgccct gggccgggct
     1861 gagcgtcagc gccggttgcg acgcgccggt cgagccggtt gccgtggttt gcggccacca
     1921 gagcgggcgc cagagcgggc ggccgaggtc ggcggcgtgg tcgacgatca ggcgctggtc
     1981 gctgccgccg gcgccgatga cgtacagctc gccgtcgtcg atcgtgccgg ccgagaggtc
     2041 gctgccctcc ggagcggcga tgacggccag ctggcgcgag tcgggcgacc agctcggagc
     2101 ggagagcgcg tagccgctga tgtcgccgag ccggtgcacg ccggccccat tggcgcccat
     2161 cgaccagagt tgcgtctcgt ggtccgcttc ggacgtcacc ggcgctcgca ggtaggcgat
     2221 cgtccagccg tccggcgacc agaccggctg gtactcgtcc tccggcgtct cggtcaggtt
     2281 gatcagcccg ctgccgtcgg tgttgatgac gtagatgtcg aagttgtcgt cccggttcga
     2341 gaagaacgcg atccggctgc catccggcga ccagtcggga gccgagtcga tggcggggtc
     2401 gctggtcagc cggcgcaggc cgctgccgtc ccggttgacg atgtagaggt cgccggctgt
     2461 ctcgaaggcg agcgcgctgc tatccggcga ccagaccatg ttggcgcccc agtcgacggc
     2521 cgccccgttg gtgatgctca cctcgcgacc gccggcggcc ggcatgacgt agatatcggt
     2581 ccgcatgtcg cgggtcgaga tgaaggcgat ccaggcgcca tccggcgacc agagcggatc
     2641 gcggttgtcg aggaagatcg agcgctggac gacaccgctg ccatcggcgt tggcgacgta
     2701 gacctgggcg gccccgcgcg agtcgctgga ccaggtcagc tgctccccgt ccggcgacca
     2761 gccaggcgac cagtgggtcg aggcgtcggt cgtcacggac gccagcccgc tgccatcggc
     2821 gttgacgacg gcgatctgcg ggacgccgtc ctggctgctg acgaaggcga gctgcccgcc
     2881 gcccggcagg tccgcgccgg ggtaggtcca gccgcgcggg ttgggcgtct cgatcgtccc
     2941 ggccgcctgt cggtcggcgt cacgcaaccc ggccagcccc tcgaagaggg caaccagcgc
     3001 gacggcgacg acggcaacag ccgcgatccc ggccacgaac tgtagccccg gatgggtccg
     3061 gccctgccca ccgccatcac gcgggatcag ccgcaagcga cccagcgaac ggctctccag
     3121 cggccgccgg ccggcgtcga gcatgccagc acgagcacct ttgcgacggc ctcgcaaccg
     3181 ccccaggtgg cgctgctgct gctcggccga cagcgactca ccagcgaagg cgcggcgcac
     3241 cgcctgcccc agctcgtcga gctcgttgtc gatgtcgggg tggttagtca cggacgatgc
     3301 ctccgtcgag gttcgggatt cgggattcgg gaaccgggat tcgggggtat tcaagggaga
     3361 atgtccgtct gacacccccg aatcccgaat cccgaacccc gatctcaaca caccacatca
     3421 ctcgtcaccc cccaactcca ccgccagccg cttgagcgcc cgttggaggc gcatcttgac
     3481 ggcgccttcg gagatgtcga ggatctcgcc gatggtcgcg atcggcagtt ccgccacgta
     3541 gcgcagcagg acgacctgct ggtgctggtc ggtgaggcgg ctgatggcct ggcggacgtc
     3601 gtcgcggcgt tcgaggcgga gcgtggtcgt cagggcgtcc tcgtcgtgct cctcgtggtg
     3661 gccgcgccgg gcaaggtagc gccagaactg gcggctcttg cggtcgcggt cggcgatccg
     3721 gcggctggcg atcgtgaaga tccagctggc gaagctgcca cgatcggggt tgaagccgtt
     3781 gattccctcc agcgccgaca tcatcgtgtc ggagacgatg tcgtcggccg ccgtcgccga
     3841 gcgggtgcgg ctgatcgcat agcggtagat acggttgctg tagcgcaggt agagctggct
     3901 gaagcactcc gcgtcggtcc gggcagcaac ggccagcgcc tcgtcgctgt ccagcggcaa
     3961 cgacgccgtt ggaacaaccg cccgcccccc gacctgtccg aagacagtcc ccttgctgac
     4021 caaccgtacc ccctctattc gatggttata tcgcgtgggg tgctatgaga gtaacgctgg
     4081 cgaggtggaa gggttgcgag gcacgatctc cgctcatcac cccacggctt gacagtgtct
     4141 gacgcctgtg ctggactgcc gcgatggcga cgccacactt cgatcgttcc gctccagcag
     4201 cggccgtcct ggtcgcggcg gggccggagc gacggagtac acgggatggg agtctgaggg
     4261 ctatgcaaca accgcagtac gacgcagtca tcgtcggcgg cgggcacaac ggactcgtcg
     4321 catcgttcta cctggccagg gccggcatga agacgctcgt gctggagcgg cgcgacttcg
     4381 tcggcggcgc ggccatcacc gaggagctgt tccccgggta ctccgtaccg agctgctcct
     4441 acatctgcta cctgctccag ccgaggatca tcgacgacat ggacctgcgc gagtacggct
     4501 tcgagatcct gccggccgcg aaggacacct tcatgccctt cccgaatggc cgttcgctcc
     4561 tcgacggccg cgagggccgc tcgattgagg aacagatcgc cgagttcgcg ccaggcgatg
     4621 ccgtcaatct cccgaagtac cgcgagatcc gccggcggat ggcgggcatc atccaccagt
     4681 cgttctttga gaaagcgccg accctgaccg aactggtcga cagcgtgcgt ggcaccgagg
     4741 acgagccact cctggagcgg ttgctcttcg gctcggtcgc cgacctgctg gacgagcact
     4801 tcgaagacga gcagatcaag ggctggctgg cgcaggcctg ggacgccggc gatatcgacg
     4861 cacccggcag cctgctctca gccgccttcc tcggcgtctc cgccttcacc cgcgacgggg
     4921 actacggtct ggtgcacggc gggatgggca cgatcacgca gtcgatggcg cgctcgtgcc
     4981 aggcgcaggg agtcgagatc cgcacgaacg cccgcgtcga gcagctgctg gtcgaggacg
     5041 gccgggcagc cggcgtgcgg ctggagagcg gcgaagagat ccgctcgcgg ctggtgctgg
     5101 tcaacgccga tatcaagcag ctcttcaaca agctggttcc gaagggggcc gtctccgacg
     5161 acttcgtgcg ggccgtcaac cggctgaagt cgaataccct ctactacaag ttccatgcgg
     5221 cgctgaacga gctgcccgat ttctcccgct ggctcggttc ggatcccgac ccgtactacc
     5281 tcggctacgt caagatctgc ccgtcgatgg attactaccg caaggcgtgg aacgacgcgc
     5341 gcaacggcct catcaccgac tcgcccgtga tgcacattca gatggcgagt gtcatcgaca
     5401 ggtcgctcgt cccgggcggc gggcatgtca tgtcgatctg ggcgctctac gccccgcaca
     5461 agccgaccgg tcacggcagc gaggtctgga cgccggagct gacccggctg gcgggcgaga
     5521 cgttcatcga ccggatggag gagtacgcgc cgaacatccg ctccgcgatc agggactggg
     5581 tgatgttcac gccgaacatc ctcgaagagc gcgtcggcct gccgaatggc cacatccacc
     5641 acatcgacca gatcgccggt cagatgttcg ccaaccggcc gctccccggc tgggccgact
     5701 accagatgcc gctcgatggg ctgtacctgt gtggcgcaga cacccaccca ggcggcgaag
     5761 tcaccggcgc caacggctac aacgccgccc acaaggtgct ggaggacgcg cgcgtcctcg
     5821 tcggctaggg agtgcatcgc gtccggctga tgcgcaggat gcgcgggacg aggtatcagg
     5881 tgacaggtat caggttttag gagtttcggc cactcgggta cgttgccggc catagaaagg
     5941 catcaggggc ttgacccagt cgggccaaac ccctgatacc tgtcacctga tgcgtatcac
     6001 ctactcgtat cgcaacgcct ccgccggtgc gatccgggct gcctggcggg aggggatcca
     6061 ggtcatcagc agcgcggcgg cgaacgtcga
//
LOCUS       DASUGJ010000416         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_342277, whole genome shotgun sequence.
ACCESSION   DASUGJ010000416 DASUGJ010000000
VERSION     DASUGJ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342277"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2006
                     /locus_tag="VFV93_09160"
     CDS             <1..2006
                     /locus_tag="VFV93_09160"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5165551.1"
                     /translation="TGAIAIDAHYDSGATGPGAMDAGSGVVTTLETLRAILAGPALRN
                     DLIVVFADAEEEGMLGAAAFNEQHPWAADVQLAINFEGAGGDGPALLYATSEQNNWLV
                     SEYLDAAPDPAATSLLAEIVNLYKAGRLDCDLGEYTDKGSAGLGFVVLGDTPVYHTIR
                     DNPDEIDSGSIQQEGANTLAAVRHFGNLDLSDTPTSGNRVFFNIWPGVVVHYAATWAL
                     ALAVVVTIIVAGLLVTGLRRGELTRRGLLAGSLAYLLGTLGAVAFAAALWFAIHALNA
                     DYQVMLIGNYQAGFYTVALALVTVAVVAALYTLLDGRIRRQNLIAGVLLGGLLPLWFA
                     SLAVPGMSYLIAWPLLFATLPLAWTLFAGRWAAQPWRMVAVLSVAAIPAIVLLPGTLH
                     AMLGLVNRLEGLSGLGLLGLLMLFVAPLAGLFVPHLHFVAGERESSRRRWALPGTIAL
                     VAIALLAWGNVTSGFDADQPRPNRIAYELNADTGQASWVSYDRQLDNWTEQFFPAGVD
                     AVDHETAMLGTLSAFVAPAPPATVALPEVVVLNDTNEGDLRTLTLNIASPANARISVV
                     DVAAPGEVVAVAVDGRQLDINGVDWARDGEFPIIYRNVPDGGWQLSLTVRSIDPITVT
                     IEETTDGLPDVPGMTIEPRPAWTMAAAGYAYDPTTVIRTFRFD"
     gene            2053..2850
                     /locus_tag="VFV93_09165"
     CDS             2053..2850
                     /locus_tag="VFV93_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165552.1"
                     /translation="MATDIVVEDRLQKATIVDRDAPVGEFLAPPSTPPAVPGKPGAFF
                     DAVRAFGVAVGPSVVFHALTLGSLPALLSRRVRRPASLGVVALAAYWLALRPWHLRWG
                     ATEEEAAGPLPGDEFEPNPAIEMTRAITIDAPVEAVWPWLAQMGQERGGFYSYEWLEN
                     LAGCRMRNAESIHPEWQQRQVGEEIKLHPAAGLNISLFEPDRAFGIQHWGTYVLKPCD
                     GGRRTRLLARARVGRGEAIFYALCIEIPHFVMERAVLLGIKERAERA"
     gene            3057..>3493
                     /locus_tag="VFV93_09170"
     CDS             3057..>3493
                     /locus_tag="VFV93_09170"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX5165553.1"
                     /translation="MARRIATKHWMMGAGAGALLLVGGLAAGPVAGLAQGDPSADLSK
                     DEAAQIAIAEYPGTTATSVDLEEEDGNAIYDVNLSNGYEVEVDGNSGQILETEASDDD
                     HEGDDSHEADDANDDDANGAGDEVRVPAGSIDDGADLLPQATI"
BASE COUNT          572 a         1154 c         1157 g          610 t
ORIGIN      
        1 gcaccggcgc gatcgcgatc gacgcccact atgactccgg tgcaaccggc cccggcgcga
       61 tggacgccgg ctcgggtgtc gtgacgacac tggagacgct gcgggcgatc cttgccggcc
      121 cggcgctccg caacgacctg atcgtcgtct tcgctgacgc cgaggaagag ggaatgctcg
      181 gcgcggctgc cttcaacgag cagcatccct gggctgccga tgtccagctg gcgatcaact
      241 tcgagggcgc cggtggcgac ggcccggccc tgctctacgc caccagcgag cagaacaact
      301 ggctcgtctc ggagtacctc gatgccgcgc ccgatccggc cgccacatcg ctgctggctg
      361 agatcgtcaa cctctacaag gcgggcaggc tcgactgcga cctcggtgaa tacaccgaca
      421 agggcagcgc cggtctcggc ttcgtcgtcc tcggtgacac gcccgtctac cacacgatcc
      481 gcgacaatcc ggatgagatc gactccggta gcatccagca ggagggcgcc aacacattgg
      541 cagccgtccg tcacttcggc aatctcgacc tgtctgacac cccaaccagc ggcaaccgtg
      601 tcttcttcaa catctggccc ggcgtcgtcg tccattacgc tgcgacctgg gcgctcgccc
      661 ttgcggtggt cgtgaccatc atcgtcgccg gcctgctggt gaccggcttg cggcgtggtg
      721 agctgacccg gcgcggcctg ctggccggat cgctggcgta cctgctcggc acgctcggcg
      781 cggtcgcgtt cgccgccgcc ctctggttcg cgatccacgc cctcaacgct gactaccagg
      841 tgatgctgat cgggaactat caggccggct tctacaccgt tgccctcgcg ctcgtgacgg
      901 tcgccgttgt cgcggcgctc tacacgctgc tggatggccg aatccgccgt cagaacctga
      961 tcgcgggcgt cctgctcggt gggctgctgc cgctctggtt cgccagcctc gccgtcccag
     1021 gaatgagcta cctgatcgcc tggccgctcc tgttcgcaac gttgccattg gcgtggacgc
     1081 tcttcgcggg gcgctgggcc gcgcaaccgt ggcggatggt cgctgttctc tcggtcgccg
     1141 caatcccggc aatcgtgctt ctgcccggca cgctccacgc gatgctgggc ctggtcaacc
     1201 ggctcgaagg gttgtcggga ctcggcctgc tcggcctgct gatgctcttt gttgcgccgc
     1261 tggccggact cttcgtcccg cacctgcact tcgttgccgg tgagcgtgaa tcctctcgcc
     1321 gccgctgggc gctgccggga acgatcgcgt tggtcgcgat cgcgctgctc gcctggggca
     1381 acgtcacctc tggcttcgat gccgaccagc cgcggcccaa caggatcgcc tacgaactga
     1441 acgcagatac cggccaggcg agctgggtca gctacgaccg gcagctcgac aactggaccg
     1501 agcagttctt cccggctggg gtcgatgccg tcgaccatga aacggccatg ctcggaacgt
     1561 tgtccgcctt cgtcgcccca gcgccgccgg cgacagtcgc cctgcccgag gtcgtcgttc
     1621 tcaacgacac gaacgagggc gacctgcgaa cgctgacact caatatcgct tcgccagcga
     1681 acgcccgcat cagcgtcgta gatgtcgccg cgccgggcga ggtcgttgcg gtcgccgttg
     1741 acggccggca acttgacatc aatggggtcg actgggcgcg cgacggcgag ttcccgatca
     1801 tctaccgcaa cgttccggac ggcggctggc agctctcact gacggtccgc tcgatcgatc
     1861 cgatcaccgt cacgatcgag gagacgacgg acggtctgcc ggatgttcct ggcatgacga
     1921 tcgagccacg cccggcctgg acgatggccg ctgcgggcta cgcctacgat ccgacgacag
     1981 taatccgcac gttcaggttc gactagctgg ttcgcatcgg gcggccggct actactggaa
     2041 caggaggaac gcatggcaac ggacatcgtc gtggaagaca ggctccagaa agcaaccatc
     2101 gtggatcgcg acgcgccggt cggagaattc ctcgcgccgc cttccacgcc tccggcggtg
     2161 cccggcaagc cgggtgcgtt cttcgacgcc gttcgtgcct tcggcgtcgc tgtcgggccg
     2221 tcggtcgtgt tccatgcgct gacgctgggc agcctgccgg cgctgctgtc gcggcgggtg
     2281 cggcgtcctg cctcgctcgg ggtcgtggcg ctggctgcct actggctcgc gctccggccg
     2341 tggcacctgc gttggggggc gaccgaggag gaagcggccg ggccgctgcc aggcgacgag
     2401 tttgagccga acccagccat tgagatgaca cgcgcgatca cgatcgacgc gccggttgag
     2461 gcggtctggc cgtggctggc gcagatgggc caggagcggg gcggattcta cagctacgag
     2521 tggctggaga atctggctgg ttgccggatg cggaatgccg aatcgatcca tcccgagtgg
     2581 cagcaacgac aggtcgggga ggagatcaag ctgcaccctg cggccggcct caacatctcg
     2641 ctgttcgagc cggaccgcgc cttcggaatc cagcactggg gaacgtacgt cctcaaaccg
     2701 tgcgatggtg gccggcggac ccggctgctg gcccgcgccc gggtcggccg gggcgaggcg
     2761 atcttctacg cgctctgcat cgagataccg cacttcgtca tggagcgcgc ggtgctgttg
     2821 ggtatcaaag agcgggccga acgggcgtaa ccagacgggt ccgcgcagat gcgaggcatc
     2881 tgcgcggttt atcgcccagg ccagcctcgc cagaactggc cgctcgtatc tacccggcgc
     2941 gaacccctca ttctcggttt atccggattt aatctgtgac cccgaagatc gttccagaca
     3001 accggtcgcg atggtcgcga ctgacggata tacagacacg agatggggat agcgggatgg
     3061 cacgacgaat cgctacgaag cactggatga tgggtgccgg ggcgggggca ttgctgctgg
     3121 ttggagggtt ggcggccggg ccggttgctg gtctcgcgca gggcgaccct tctgccgatt
     3181 tgagcaagga cgaggctgcc cagatcgcga tcgccgaata tccaggaacg acagcgacga
     3241 gcgtcgatct cgaagaagaa gatggcaacg cgatctatga tgtcaacctc tccaacggct
     3301 acgaagtcga ggttgatggc aacagcggcc agattctcga aactgaagcc tcggacgacg
     3361 accacgaggg cgacgatagc catgaggcag acgacgccaa cgacgatgac gccaatggcg
     3421 ctggagatga agtacgtgtc ccagccggca gcatcgacga tggcgctgat ttgctgccac
     3481 aggcgacgat cag
//
LOCUS       DASUGJ010000417         6565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1175771, whole genome shotgun sequence.
ACCESSION   DASUGJ010000417 DASUGJ010000000
VERSION     DASUGJ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175771"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..824)
                     /locus_tag="VFV93_09175"
     CDS             complement(189..824)
                     /locus_tag="VFV93_09175"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5165554.1"
                     /translation="MSATITVAGTAVVPAQPDEVAIGIALSHLAPTPDAAITEVAARS
                     RQLETIFDEMQIGRDRWTTSGVSVNEEREWDGQRQIHRGYRAGTRVQLRVADAELVGK
                     VMHESVRRCQASIDGPTWLIAPDNPAHDDARRRAATSARAKAETYAAALGVRLGTILS
                     VTEPGLSAAPPSPMPKVMRTMAMDAAPEITVQSGDLDISATVIVTFGLEQG"
     gene            complement(855..1652)
                     /locus_tag="VFV93_09180"
     CDS             complement(855..1652)
                     /locus_tag="VFV93_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX5165555.1"
                     /translation="MPNPTAVRSLTILDFGRANIDLGAVMAPGDMDGKWAVCAFPGYL
                     VTLDDGRQILIDTGPNRRHIREPMFEFAGTSFGERLQPQMTEADDPRNRLAEMGLTEA
                     DIDILVLTHTHFDHAGNAADFTSSEIVIHRDAYADGRDKAERGVPGGLPEVGADGRPL
                     NFRIIDGDTELVPGVTLLQTPGHANGHLSVLLRLPQTGTVILAIDAIYSRANRDASNY
                     KVGANPSQGLASADRLIALAEQENALLIYGHDPQQWQQLRKAPDQYT"
     gene            1708..1944
                     /locus_tag="VFV93_09185"
     CDS             1708..1944
                     /locus_tag="VFV93_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165556.1"
                     /translation="MDEQRNPQHESDQEIDDTSEATARPKTMFDAAEEDQLVLDEMKR
                     FERKRAAIWGLAAGGIMSLIIVICAVLIYLLGTR"
     gene            complement(1941..2504)
                     /locus_tag="VFV93_09190"
     CDS             complement(1941..2504)
                     /locus_tag="VFV93_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5165557.1"
                     /translation="MTPARMPVTLLLTAWDDLDRAVADVSEADMLRQIDGGSCFAWTI
                     AHVTYGVDSWINRRFQKIPLNPVVIDPRFNFGGDGRTDDWPTIRDAVAEVRASLRPYL
                     LNLTDADLDLTVPYDGSYAAFRERGINLRVAIIQNAIHHVFHLGEIVAKRELMGYDPG
                     SFPGAFGAALMGEEPLPQPPPASQHAR"
     gene            2694..3272
                     /locus_tag="VFV93_09195"
     CDS             2694..3272
                     /locus_tag="VFV93_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165558.1"
                     /translation="MVQAERMNGLIRDVDEAGFLSEGHFAFRSGQHALRLLDRDRLLC
                     DTQLASRMGYAMAKHFFLSRIDIVAAPSVWGAGLAQWVGYFLDPRRPVIYPIPANGHY
                     TFSDSSKEMIDGKRVLVIDNLILSGKTAQELVQTITANGGTPIGIGALADLSGIDFPI
                     AVHGLLNEHLTIHAPDGCPACAAGMPLTEVGY"
     gene            3529..5631
                     /locus_tag="VFV93_09200"
     CDS             3529..5631
                     /locus_tag="VFV93_09200"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5165559.1"
                     /translation="MYRCKVCGYIETDAVQPEECPSCGAASDRFTPITEADLNSYMAG
                     QISYGYVGQAVDETPVDDTRRIRLLAFVDQENFSPFTVRQGSRYPVFTGDQRWFADNI
                     PCQRACPAHTDISRYISFIADGRYADSFELNRENNVFPGCLGRMCAKPCEDACRRQVI
                     DQPIGICYLKRVAADFRGETKREVAPPPNGKTVAIIGAGVAGMSSARELARRGYKVTI
                     FEQFAVPGGMLWGGVPEWRLPRDIIMEEIELILDLGIDLKLNTKIGVDVMMEDLVNDY
                     DAVVIAAGNQNPSKLHVPGEDLPGVVPGLTFLEEVNLNLVDDVFVGKTVVTVGGGFTS
                     IDCMRTVLRMGAERSVMTYRRSHNEIPVEEREIEESELEGCEVMYMVAPTRCIAGPDG
                     HVAGIEFVHNELGPPDASGRRRPSPIEGSEFIIDCDMVLVAIGQYQDNDFFPDGFFPE
                     VDRRGVPRIDATGATQVQKVWACGDYVTNPLNFISAIGDAKRVAEEIHAALSGEQVPV
                     KDFEITRVPIEDYHTPNPLMAEGVSEWSLSSMSRRLRWGDNYTSLERQVMPTLPLSAR
                     GLLADETTIEVDLGYPKPVGFNEAKRCLQCQLNIFIDGDRCILCNGCVDICPVGVIEM
                     ISLDRIFSIDNDEELAEMARGELGRNAVAMVIDELACIRCGACVEWCPTECLTMEHYR
                     PTPLPARESTDLAIVADG"
     gene            complement(5715..6365)
                     /locus_tag="VFV93_09205"
     CDS             complement(5715..6365)
                     /locus_tag="VFV93_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5165560.1"
                     /translation="MSPRPYNAERRQVAAEETRQRILDAACALLASAEPPRSFSVEAI
                     ARQADVARMTVYYQFGSKPGLLEALFDHLATRAEIGARLRAAFSQPDPVASLHAVIAA
                     FAHFWTLDRLIIRRLHALAVLDPELEPRERARNERRRTALRRVLEQLRDGEHLEQQTD
                     PDGILDTLQGLTSFETFDHLAGPDRTPEDVIPIVCRIADSLLGVGDAVRDSIVLPS"
BASE COUNT         1132 a         1996 c         2266 g         1171 t
ORIGIN      
        1 cgcgacgcag ggtattcgga cacggagatc gacgggctga aggcgcaagg ggttatatcc
       61 gtcggataac ggccctcacc ccccgacacc ctctccccat ttctgggaga gggggagcag
      121 gaactgagtg tcggcagccg ctccgcttct ctcaaatctg gcagaggggg agggaggagt
      181 gagggccgtt atccctgctc cagcccgaat gtcacgatga cggttgcact gatgtcgagg
      241 tcgccggatt ggacggtgat ctcgggggcg gcgtccattg ccatcgtgcg catgactttt
      301 ggcatcgggc ttggcggggc ggcgctgaga cccggttcag tgaccgacag gatcgtgccg
      361 aggcgaacgc ctaacgcggc cgcgtacgtc tcagccttgg cgcgggcgga ggttgcggcg
      421 cggcgacggg cgtcatcgtg ggccgggttg tcgggcgcga tcagccaggt tgggccgtcg
      481 attgacgcct ggcagcgacg aactgattcg tgcatgacct tgccgaccag ctctgcgtcg
      541 gcgacacgca gctggacgcg ggtgccggcc cggtagccac ggtggatctg gcgctgaccg
      601 tcccactcgc gctcttcgtt gaccgagact ccggatgtcg tccagcggtc gcggccgatc
      661 tgcatctcgt cgaagatcgt ctcaagctgc cggctgcgcg ccgcgacctc ggtgatggca
      721 gcatccggcg tcggcgcgag atgcgacaac gcgatgccga tcgcgacttc gtccggctgg
      781 gccggcacga cggctgttcc ggcgacggtg atcgttgcgg acatgcttcc tcctcatcgt
      841 cgacgcgtcg tcagctaggt gtattgatcc ggtgctttgc ggagttgctg ccattgttgc
      901 gggtcgtggc cgtagatcag gagcgcgttc tcctgctcgg ccagtgcgat cagccgatcg
      961 gctgaagcca gtccctggct gggattcgcg ccgaccttgt agttggacgc gtcgcggttg
     1021 gcgcgcgagt agatcgcgtc gatcgccaga ataacggtac cggtctgcgg caagcgcagc
     1081 agaaccgaga gatggccgtt ggcgtggcca ggcgtctgca ggagcgtgac gccgggtacc
     1141 agttccgtgt caccgtcgat gatgcggaag ttgagcggcc ggccgtcggc gccgacctcc
     1201 ggcaagccgc caggaacgcc gcgctcggct ttgtcgcggc catcagcgta ggcgtcgcgg
     1261 tggataacga tctcggatga ggtgaaatca gcggcgttac ctgcgtggtc gaagtgggta
     1321 tgcgtcagga cgaggatgtc gatatcggcc tcggtcaatc ccatctccgc caggcgattg
     1381 cgagggtcgt cggcctcggt catctgcggc tggaggcgtt cgccgaagct ggtcccggcg
     1441 aactcgaaca tcggctcacg aatgtggcgg cggttggggc cggtgtcgat caggatctgg
     1501 cgaccgtcgt cgagcgtaac caggtagccg gggaaggcgc agacggccca tttgccgtcc
     1561 atgtcgccag gcgccatgac ggcgccgagg tcgatgttgg cgcgaccgaa gtcaagaatc
     1621 gtcaatgagc gaacggcagt tgggttgggc atgttatgct cccgttcctg aggccgatac
     1681 gacaccagca ggcaggagtc cacccgggtg gacgaacagc gtaatccgca acacgagtca
     1741 gatcaagaga tcgacgacac cagcgaggcg acggcgcgcc cgaagacgat gttcgacgcg
     1801 gccgaagagg atcagctggt gctggacgag atgaagcgct tcgagcgcaa gcgagccgcc
     1861 atctgggggc tggccgccgg cgggatcatg tcgctcatca tcgtcatttg cgcggtgttg
     1921 atctatttgt tgggaactcg ctagcgggcg tgttgagacg cgggtggcgg ttgggggagg
     1981 ggttcttcgc ccatcaacgc cgccccgaag gcaccgggga aactgccggg gtcgtagccc
     2041 atcagctccc gcttggcgac gatctcgccg aggtggaaga cgtggtggat cgcgttctgg
     2101 atgatggcga cgcgcaggtt gataccgcgc tcgcggaagg cggcgtagct gccgtcatac
     2161 ggcacggtca ggtcgaggtc ggcgtcggtc aggttcagca gatacgggcg caggctggcg
     2221 cggacctcgg cgacggcgtc gcgaatggta ggccagtcgt ccgtacggcc atcgccgccg
     2281 aagttgaagc gcgggtcgat cacgaccgga ttgagcggga tcttctggaa gcggcgattg
     2341 atccaagagt cgacgccgta ggtgacgtgc gcaatcgtcc aggcgaagca gctcccgccg
     2401 tcgatctggc gcagcatatc ggcctcgctg acatcggcga cggcgcggtc gaggtcgtcc
     2461 caggcggtca gcagcagcgt cacgggcatc ctcgctggcg tcatccgcgg ttcctctcag
     2521 gtgtgatcgt cgtttgattg cggccgactg tcgcacacac aagccgctca tgtaaacccg
     2581 gctcattgca ggttgccgga tcggcgcgtc tgccgtacgc tgggagtggt ttcccgcatg
     2641 acagacaagc gactacggcg gcacgagcgt cgccggcgat gggaggaacc acgatggtgc
     2701 aggccgaacg gatgaacggg ctgatccgcg atgtcgatga ggcaggcttc ctgagtgagg
     2761 ggcatttcgc ctttcgatcg gggcagcatg cgctgcggct gctggatcgc gaccggttgc
     2821 tgtgcgacac ccagcttgcc agccggatgg gctacgcgat ggcgaagcac ttcttcctgt
     2881 cgcggatcga catcgtcgcg gcgccatcgg tctggggcgc cggactggcg cagtgggttg
     2941 gctatttcct cgatccacga cggccggtga tctatccaat cccggccaac ggtcattaca
     3001 cgttctccga ctcctcgaag gagatgatcg acgggaagcg tgtgctggtg atcgacaacc
     3061 tgatcttgtc tggcaagacg gcccaggagc tggtccagac gatcacggcc aacgggggga
     3121 cgccgatcgg catcggtgcg ctggccgacc tctcaggcat cgacttcccg atcgcggtcc
     3181 acggactgct caacgagcac ctgacgatcc acgcgccgga tggctgcccg gcctgtgcgg
     3241 cggggatgcc attgacggaa gtcggctact gaccggtacg tcccactgca aggcgaggtg
     3301 tcgccaacac ccgtctgcgc atgagtgaga aatgtccgta tttgcggtga cagcggctgt
     3361 atacttcgtc tgacggcgcg ccgctccgaa atggtcgcat gtgtggcggg ccgccggtac
     3421 ggctgctacc gcatcaccgt cgtgcatccc tgaagccgtc acgaacgata catccgcggc
     3481 aacgggccgc gttaccggac tatccggccg ggcaggggag gaaccgtcat gtatcgatgt
     3541 aaggtctgcg gctatattga aaccgatgct gtccagcccg aggagtgtcc aagctgcggc
     3601 gcggccagcg accggttcac gccgatcacc gaagcggatc tcaactccta catggccggg
     3661 cagatctcgt acggctatgt cgggcaggca gtcgacgaga cgccggtcga tgacacgcgg
     3721 cgcatccggt tgctggcgtt cgtcgaccag gagaacttct cgccgttcac cgttcgccag
     3781 gggagccgct atccagtctt caccggcgat caacgctggt tcgccgacaa catcccctgc
     3841 cagcgcgcct gcccggccca caccgacatc tcgcgctaca tctccttcat cgccgatggg
     3901 cgctacgccg actcattcga gctgaaccgg gagaacaacg tcttccccgg ctgcctcggc
     3961 cggatgtgcg ccaagccttg cgaggacgcc tgccgccggc aggtcatcga ccagccgatc
     4021 ggtatctgct acctgaagcg cgtcgcggcc gacttccgcg gcgagacgaa gcgcgaggtc
     4081 gcaccgcctc cgaacggcaa gacggtcgcg atcatcggcg caggtgtggc aggcatgtcg
     4141 tctgcgcgcg agctggcgcg gcgcgggtac aaggtcacga tcttcgagca gttcgcagtg
     4201 ccgggtggga tgctctgggg cggtgtccct gagtggcgcc tgccgcgcga catcatcatg
     4261 gaagagatcg aactgatcct cgacctcggt atcgatctga aactcaacac caaaatcggt
     4321 gtcgacgtga tgatggaaga cctcgtcaac gactacgatg ccgttgttat cgcggccggc
     4381 aaccagaatc cgtcaaagct tcatgtgcct ggcgaggatc tgccgggggt cgtgccgggc
     4441 ctgacgttcc tcgaagaagt caacctgaac ctggttgacg atgtcttcgt cggcaagacg
     4501 gtcgtcaccg ttggcggtgg cttcacctcg atcgactgca tgcgaaccgt cctgcggatg
     4561 ggcgccgagc ggtcggtgat gacctaccgg cggtcgcaca acgagatccc ggtcgaagag
     4621 cgcgagatcg aggagtcgga actcgagggc tgcgaggtca tgtacatggt cgcgccgact
     4681 cgctgtatcg cgggacccga cgggcatgtg gccggcatcg aattcgtcca caacgagctt
     4741 ggcccgccgg atgcatccgg ccgccggcgt ccgtcgccga ttgaaggatc ggaatttatc
     4801 atcgactgcg acatggtgct ggtcgcgatc ggccagtatc aggacaatga tttcttcccg
     4861 gatggcttct tcccggaggt cgaccggcgc ggtgtgccac gcatcgacgc gaccggcgcg
     4921 acccaggttc agaaggtctg ggcctgcggc gactacgtga cgaacccgct caacttcatc
     4981 tcggccatcg gcgatgccaa gcgggtcgct gaggagatcc acgcggcgct gtccggcgag
     5041 caggtcccgg tcaaggactt tgagatcacg cgggtaccga tcgaggacta ccacacgccg
     5101 aacccgctga tggcggaggg cgtctcggag tggtcgctct cgtcgatgag ccgccgcctg
     5161 cgctggggcg acaactacac ctcgctcgaa cggcaggtga tgccgacgct gccactctcg
     5221 gcgcgcggcc tgctggcgga cgagacgacg attgaggttg acctgggcta cccgaagccg
     5281 gtcggcttca acgaagccaa gcgctgcctg caatgccagc tcaacatctt catcgacggt
     5341 gaccgctgca tcctctgcaa cggctgcgtc gatatctgtc cggtcggcgt gatcgagatg
     5401 atctcgctcg accgcatctt ctcgatcgac aacgacgagg aattggcaga gatggcgcgc
     5461 ggcgagttgg gccgcaacgc ggtcgcgatg gtcatcgacg agctggcctg catccgctgc
     5521 ggcgcctgcg tcgagtggtg cccgaccgag tgcctgacga tggagcatta ccggccaacg
     5581 ccgctcccgg cccgcgaatc gaccgacctg gcgatcgtcg cggacggcta gcagccagga
     5641 acgctgagat tgcaacgggc ggggccgatc ggccccgccc atttgcgcta tcaacctctc
     5701 gtggggatcg gtccctatga gggcagaacg atgctgtcgc ggaccgcgtc gccgacgccg
     5761 agcaagctat ctgcaatccg gcagacgatt gggatgacat cctcaggcgt tcgatcggga
     5821 ccggcgaggt ggtcgaaggt ctcgaagctg gtcaggccct ggagcgtgtc gaggatcccg
     5881 tccggatctg tctgctgctc caggtgttca ccgtctcgga gttgttcgag cacccggcgc
     5941 aaagccgttc ggcggcgttc gttgcgggcg cgctcgcggg gctccagctc ggggtcgagg
     6001 acagccagcg cgtgcaggcg gcggatgatg aggcggtcaa gcgtccagaa gtgggcgaat
     6061 gcggcgatca ccgcatggag cgaggcgacc ggatcgggct ggctgaatgc ggcgcgcagc
     6121 ctggcgccga tctcggcgcg ggtcgccaga tggtcgaaga gagcttccaa caagccgggc
     6181 ttcgagccga actggtagta gaccgtcatc cgagcgacat cggcctggcg agcgatcgcc
     6241 tcgacgctga agctgcgtgg cggttcggcg gaggcgagca gggcacaggc cgcgtcgagg
     6301 atgcgctggc gcgtctcctc ggccgccact tgacgccgct cggcgttata cggtcgtgga
     6361 ctcatcgtaa ccgggtccct tttctacaga acagatctcg acgccgttcc cctgtcgtcc
     6421 accgcccaga gggcacccgg cagcgaaaga cgacagggga acggccccgc caggtggtcg
     6481 ctggcgcggc gggcggagtg gctctgggca acgttctttg tatgtattat acaatgaaat
     6541 ttgtcacgga aggaggtggc agctt
//
LOCUS       DASUGJ010000418         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1732416, whole genome shotgun sequence.
ACCESSION   DASUGJ010000418 DASUGJ010000000
VERSION     DASUGJ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732416"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..754)
                     /locus_tag="VFV93_09210"
     CDS             complement(296..754)
                     /locus_tag="VFV93_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165561.1"
                     /translation="MQNKRSAGVRAESLLGVMIRRSTPWAAAAGVVCLITYSVIDGWS
                     GALASLVGVGFVAFFFGVDLLVLRLTRGAPGGVTAAALMGEYLIKVLVMAGVLWLLSE
                     STSLDLQVTAVTVVVTTVIWAVAVTAVALRTRSFFFDFPSATNTESRSDQ"
     gene            complement(757..1857)
                     /locus_tag="VFV93_09215"
     CDS             complement(757..1857)
                     /locus_tag="VFV93_09215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX5165562.1"
                     /translation="MREYLLVLLASAAITYVTVPVVRWYAVRWNVMAEIRDRDVHDIP
                     IPRLGGVAMYLGFLAGMLVASQLGFVSAVFSESRDAWAVITGGTLIFLVGVVDDVWGL
                     DALTKAAGQIIAAGVMVLQGVQLLWLPFNGVTVLDYNSSFILTVLLVVVTVNAINFID
                     GLDGLAAGIIGIGSLGFFAFAYLLSVERGIDRATTATLLSAVLVGLCAGFLPHNFNAA
                     RIFMGDSGSMLAGLLLSASVITLTGKIDPFSVGDAALLPALMPLVLPFLVLAVPLMDL
                     LLAVARRTRARRSPFAPDKQHLHHRLLEIGHSQRKAVLIMYVWAGALAVGTAIAAFQG
                     VRIGLVIGIGLLLVAVILTRRPVRAIRVEGSE"
     gene            complement(1906..>2539)
                     /locus_tag="VFV93_09220"
     CDS             complement(1906..>2539)
                     /locus_tag="VFV93_09220"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5165563.1"
                     /translation="LPSPVPYADVVSFTTHKVLRGPRGGMILCKEELASAIDKAVFPF
                     LQGGPLMHVIAAKAVALKEASMPAYQEYARQVIANAQALVAGLQDEGMRPVSGGTETH
                     LALIDLRGVDVTGRDAELRCDAAKITLNKNAIPFDPAKPMIASGIRVGTPSVTTQGMG
                     QVEMKEVASLIGRAVRADLETDAGQSEIAQVADAVSALVSKYPAYPDPAA"
BASE COUNT          520 a          861 c          794 g          364 t
ORIGIN      
        1 acgatgccga tcggcgtcat cgcggtcagg cccagccagc ggtcgagcgc cacgccgatc
       61 cccccgaaga ggaggagccc ggcgagcagg tagccaccag ccgcttctac agcaacggag
      121 ggagcacctg agctcgtagc gccggcattg ccaggttggt ctgcctgagc catggtgcga
      181 ggaatctagc agtccggcgt agagcgccac aaagtgagtc ccgcacgtgc gctgtgagtc
      241 cgctcacgtg ccagcgaccc ggcgcgattc cccggcgaat tcaggggatt cgggcctact
      301 gatcgctccg cgactcggtg ttcgtcgcgc tcggaaagtc gaagaagaac gaccgcgtac
      361 ggagcgctac tgccgtgacg gcgaccgccc agatcaccgt cgtgacaaca acggtgaccg
      421 cggtcacctg caggtcgagc gacgtggact ctgacaagag ccacaagacc cctgccatga
      481 cgagcacctt gatcaggtac tcacccatca gtgcggcagc agtcacaccg cccggagccc
      541 cgcgggtgag ccggaggacc agcaggtcca ccccgaagaa gaaggccacg aatccgacgc
      601 cgaccagcga agcgagcgcg ccggaccacc cgtcgatcac cgagtacgtg atcaggcaga
      661 ccacgcctgc ggcagccgcc catggcgtgg aacggcggat catcacgccg agcaggctct
      721 cggcccgcac cccggccgac cgcttgttct gcacactcac tccgaaccct cgaccctgat
      781 cgcccgaacc ggccggcggg tcaggatcac ggcgaccagc aacaggccaa tgccgatgac
      841 cagcccgatc cgaaccccct ggaacgcggc gatggccgtt cctaccgcca atgcgcccgc
      901 ccagacatac atgatgagaa cggccttccg ctggctgtgc ccgatctcga ggaggcgatg
      961 gtgaaggtgc tgcttgtcgg gtgcgaacgg tgaccgccgc gcgcgtgtcc gtcgagcgac
     1021 tgccagcagc agatccatga gcggcacagc cagcaccagg aatggaagca ccagtggcat
     1081 cagagccggc aacagtgccg cgtcgcccac cgagaagggg tcgatctttc cggtcaatgt
     1141 gatgacagac gccgaaagca gcaaccctgc gagcatcgaa ccggaatccc ccatgaagat
     1201 ccgggcggcg ttgaagttgt gcggcaggaa gcccgcacag agtccgacga gaaccgccga
     1261 gagcaacgtg gctgtagtag cgcggtcgat cccgcgttcg acggacaaca gataggcgaa
     1321 ggcgaagaac cccaaggacc cgatcccgat gattccggca gccaatccgt cgagaccgtc
     1381 gatgaagttg atggcgttca ccgtgaccac gaccagcagc acggtcaaga tgaagctgga
     1441 gttgtagtcg agcaccgtga cgccgttgaa cggaagccac agcagctgca caccctgcag
     1501 caccatcact ccagccgcga tgatctgacc ggcagctttc gtgagcgcgt caagacccca
     1561 gacgtcgtcg accacaccga ccagaaagat cagcgtgcca ccggtgatga ctgcccacgc
     1621 gtcgcgcgac tcgctgaaga ccgccgagac gaagccgagc tggctcgcga cgagcatgcc
     1681 tgccaggaat cccagataca tcgcgacgcc accgaggcgc ggaatcggga tgtcgtggac
     1741 gtcccggtcg cggatctccg ccatgacgtt ccagcgcacc gcgtaccagc gaaccactgg
     1801 cacggtcaca tacgtgatcg cggccgaggc gagcagcacc agcaggtact cgcgcacgcg
     1861 gcgtcccttc agccctgccc gtccgaccgg acgggcaggt acaggctagg ccgcggggtc
     1921 ggggtacgcg gggtacttcg acaccagtgc cgacaccgcg tcggcgacct gggcgatctc
     1981 ggactgcccg gcgtccgtct cgagatcggc ccggaccgca cggccgatca gcgaagcgac
     2041 ctctttcatc tcgacctgac ccatgccctg cgtggtgaca ctcggcgtgc cgacgcggat
     2101 gccagaggcg atcatcggct tggccgggtc gaacgggatg gcgttcttgt tcagtgtgat
     2161 ctttgccgca tcacagcgca gctccgcatc tcgtcccgtg acgtcgacgc cacgaaggtc
     2221 gatcagagcc aagtgcgttt ctgtgccacc agacactgga cgcattccct cgtcttgcag
     2281 acccgccacg agcgcctgcg cattcgcgat gacctgtcgc gcgtactcct ggtaagcggg
     2341 catcgacgct tccttgagcg cgaccgcttt tgcagcgatc acgtgcatca gcggcccacc
     2401 ctgcaggaac gggaagactg ccttgtcgat cgcgctggca agctcttcct tgcaaaggat
     2461 catgcccccg cgaggaccgc gcagaacctt gtgcgtcgtg aacgacacga cgtcggcgta
     2521 gggaaccggc gaaggaagt
//
LOCUS       DASUGJ010000419         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_204025, whole genome shotgun sequence.
ACCESSION   DASUGJ010000419 DASUGJ010000000
VERSION     DASUGJ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204025"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFV93_09225"
     CDS             complement(<1..489)
                     /locus_tag="VFV93_09225"
                     /inference="COORDINATES: protein motif:HMM:NF020553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEX5165564.1"
                     /translation="MATTAAEPIQATWKVSQVLQRYPDLLDVFIATDPAFSKLRNPVL
                     RRVQSRLVTVEQAARIAGIEPPELIRTLNQHLGLEPDDEETVANPADAPVASPPPWVG
                     AAPVAVELDVREHQRRGEEPFGPIMEAVRQVPPGQVLVLRNTFDPIPLYDVLGMRGFE
                     HWA"
     gene            complement(495..806)
                     /locus_tag="VFV93_09230"
     CDS             complement(495..806)
                     /locus_tag="VFV93_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX5165565.1"
                     /translation="MSLLISEGTVREALREVYDPELGVNVVDLGLIYAIDVDGALVRV
                     TMTLTTPGCPLHDTITEAVNEAVGHYIPGVEGIQIDLVWEPRWSPEMMTEAGRREIGW
                     W"
     gene            complement(826..1071)
                     /locus_tag="VFV93_09235"
     CDS             complement(826..1071)
                     /locus_tag="VFV93_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5165566.1"
                     /translation="MTYVIAQPCINVKDASCVEVCPVDCIHSDDESDQYFIDPSECID
                     CGVCAEVCPVEAIFFEDDLPEQWNSFLQLNREYFENR"
     gene            complement(1068..1346)
                     /locus_tag="VFV93_09240"
     CDS             complement(1068..1346)
                     /locus_tag="VFV93_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165567.1"
                     /translation="MVSTAEAHAGRLARFFQRSSGPACAIDFYTLQPAPTQWVAVGRP
                     VTTDGRRPLLVGTGTTEEAAVLDLRGRCPHGEPAMHVLMRREPEDRLL"
     gene            complement(1339..1575)
                     /locus_tag="VFV93_09245"
     CDS             complement(1339..1575)
                     /locus_tag="VFV93_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEX5165568.1"
                     /translation="MQQGAFSISGDTLVEEILDRIPLAAQVFVGRRMHCVGCPIARFE
                     TLAEACRIYCQALDNMLADLSAVADSGNGGGNHG"
     gene            complement(1708..>2254)
                     /locus_tag="VFV93_09250"
     CDS             complement(1708..>2254)
                     /locus_tag="VFV93_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5165569.1"
                     /translation="KVVRETGDGQEVILRLIQPGEIFGGAGGWGEPAYPATGVTLEDS
                     TILQLPAADFTALVSEQPTFALLMIRELGSRLREAEARIRELQAERAERRIARALLRL
                     VSKTGTRTPAGIEVGVPLSRQNLAELAGTTLSTASRILSAWDRQGLIIAGRERVTIVK
                     PHRLVMIADELGGDERVPLSS"
BASE COUNT          372 a          773 c          734 g          375 t
ORIGIN      
        1 ggcccagtgc tcgaatccgc gcatgcccag cacgtcatag agcgggatcg gatcgaacgt
       61 gttgcgcagc accagcacct gccccggcgg cacctgccgg acggcctcca tgatcggtcc
      121 gaacggctcc tccccacgcc gttggtgctc gcgaacatcc agctcgacgg cgaccggcgc
      181 ggctccgacc cacggcggtg gcgacgctac cggcgcgtcg gcagggttcg cgaccgtctc
      241 ttcgtcgtcc ggttccagcc cgagatgctg gttgagtgtc cgaatcagtt ccggcggctc
      301 gatgccggcg atccgggcgg cctgctcgac cgtcaccagc cggctctgga cccggcgcag
      361 gaccgggttc cgcagcttgc tgaaggcagg gtcggtcgcg atgaacacgt ccagcaggtc
      421 cgggtaacgc tgcaggacct ggctgacctt ccaggtcgcc tgaatcggct cggctgctgt
      481 cgttgccatc agtatcacca ccatccgatc tcgcgccggc cggcctcggt catcatctcc
      541 ggcgaccagc gcggctccca gaccagatcg atctggatac cttcgacgcc ggggatgtag
      601 tgcccaaccg cctcattgac cgcctcggtg atcgtgtcgt gcagcgggca gccgggcgtc
      661 gtcagcgtca tcgtgacgcg cacgagcgcc ccatcgacat cgatggcgta gatcagcccg
      721 aggtcgacga cgttgacgcc cagctcgggg tcgtagactt cgcgcagcgc ctcgcggacg
      781 gttccttcgc tgatgagcag cgacatgctc gttcgactcc attcgtcaac ggttctcgaa
      841 atactcgcgg ttgagctgca ggaaggagtt ccactgctca ggcaggtcat cctcgaagaa
      901 gatcgcctcg accggacaga cctccgcgca gacaccgcag tcgatgcact cactcgggtc
      961 gatgaaatac tggtcggatt cgtcatccga atggatgcaa tcgaccggac agacctcgac
     1021 acaggaggcg tccttcacgt tgatgcaggg ttgggcaatc acgtatgtca cagcagccga
     1081 tcctccggct cacggcgcat caggacgtgc atggcgggtt cgccatgcgg gcagcgcccg
     1141 cgcagatcga ggacggccgc ctcttcggtc gtgccggtcc cgaccagcag cggccggcga
     1201 ccatcggtcg tgaccggccg gccgacagcg acccactgcg ttggtgcagg ctgaagcgtg
     1261 tagaagtcga tcgcgcaggc agggccgctg ctccgctgga agaaccgcgc cagtcgtccg
     1321 gcatgggctt ccgctgtact aaccatggtt tcctcccccg tttccggaat ccgcgacggc
     1381 gctgagatcg gccagcatgt tgtccagcgc ctggcagtag atccggcagg cttccgccag
     1441 cgtctcgaag cgcgcgatcg ggcagccgac gcaatgcatc ctgcggccca cgaagacctg
     1501 cgcggccagc gggatgcggt ccagaatctc ctcgacgagg gtgtcgccgc tgattgaaaa
     1561 cgcgccctgc tgcatcacag cccgccatct cattccagcg tcagactgac gcctgagatg
     1621 aggatgacac gcaggcggcc ggaggtcttt gcgccagcgc aaagagcagc atgctctcag
     1681 cgagtccccc tgtcgtcctg cgctgcgtta ggacgacagg gggactcgct cgtcgccgcc
     1741 gagttcgtcg gcgatcatga ccagccggtg gggcttgacg atcgtgacgc gctcgcggcc
     1801 ggcgatgatg agaccttggc ggtcccaggc gctgaggatg cggctggcgg tgctgagggt
     1861 cgtcccggcc agctcggcca ggttctggcg cgagagcggc acgccgacct cgatgccggc
     1921 cggagtccgg gtgccggtct tcgagaccag ccgcagcagg gcgcgggcga tccgccgttc
     1981 agcgcgctcg gcctgcaatt cacggatccg cgcctcggcc tcgcgcagcc gcgagccgag
     2041 ctcgcggatc atcaacagcg cgaaggtggg ctgctcggag accagcgccg tgaaatcggc
     2101 ggccggtagc tgcaggatcg ttgaatcctc aagcgtgaca ccggtggcag gataggccgg
     2161 ctcgccccag cctcctgccc cgccgaagat ctcgcccggc tgaatcagcc gcaggatcac
     2221 ttcctgcccg tcacccgtct cccgaacgac cttg
//
LOCUS       DASUGJ010000420         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2010141, whole genome shotgun sequence.
ACCESSION   DASUGJ010000420 DASUGJ010000000
VERSION     DASUGJ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010141"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VFV93_09255"
     CDS             <1..1147
                     /locus_tag="VFV93_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5165570.1"
                     /translation="EAVPIVKGSEINDSPYEPISRYAIDLIEAEGDKTPIDWIGRDRR
                     YAEKLATPDTTIADLIGEVDPIKVAEGRYLSDELTIHYGMIPRVNRGIFTLNELPDLA
                     ERIQVGLLNIMEERDVQIRGYRVRLPLDVYVVASANPEDYTNRGRIITPLKDRYGSQI
                     RTHYPTTTELEIDIMEQERMTYESDGVTVQMPQYMREIIAEITHLARRSHDISQRSGV
                     SVRMSIANYENVVANALKRAIRTNEKQAVPRISDLRAIVASTTGKIELETMGEVREDR
                     IVEKLIQGAIVTTFNRYFSVRDFDDLILGFDNGLIVDAADDMPSMNYVHQVSHMEGVR
                     EAIGKLDIAGSPAGIASGVEFILEGLHLNRRLNKDHSASASGSRYKA"
     gene            1267..>2558
                     /locus_tag="VFV93_09260"
     CDS             1267..>2558
                     /locus_tag="VFV93_09260"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX5165571.1"
                     /translation="MARQHVDTTEDDPANPALPSAIVRVQLLGGFRLEVGSHSVDEFN
                     WRLRKARSLVKLLALAPRHRMHREQVLDLLWPDHDPEAAGNNLRSTLHVARRMLEQHA
                     GPVPAIAVRADYLFLFPDHDVWSDVEAFEDAATAARLTRDPDSFLIATSHYRGELLPE
                     DRYEPWAEERRERLRLTWLSLLRDLTALYEQRGEQRSAIDVLLQMTSAEPADEAAHVA
                     LMRLYAQTGQRLQALRQYQHLRDQLQQVLDVEPEPESTRLYEDILAGRFDTISPATEP
                     PVTTTVIDDHRPSAPPILVPVELTSFVGRVPELDEICDLVRRSRLVTLTGPGGSGKTR
                     LAIAAARRRADEFVGGVWFVDLAPLADPALGVAEVAGRLGVAGQADRSLIDDVGDVLR
                     DRSALIVLDNCEHLIDTCAGLADALLRAAASLTILATSR"
BASE COUNT          481 a          853 c          799 g          425 t
ORIGIN      
        1 cgaggccgtg ccgattgtca agggcagcga gatcaacgac agcccatacg agccgatctc
       61 ccgctacgca atcgacctga tcgaggccga gggcgacaaa acgccgatcg actggatcgg
      121 ccgcgaccgg cgctatgccg agaagctggc gacaccagac acgacaatcg ctgacctgat
      181 cggcgaggtc gacccgatca aggtcgccga ggggcgctac ctctccgacg agctgacgat
      241 ccactacggg atgatcccgc gcgtcaatcg cggcatcttc acactcaacg agttgcctga
      301 cctcgccgag cgcatccagg tcggcctgct caacatcatg gaggagcgcg acgtccagat
      361 tcgcggctac cgcgtccggc tgccgttgga tgtctacgtc gtcgccagcg ccaaccccga
      421 agactacacg aaccgcggcc ggatcatcac gccgctcaag gaccgctatg gttctcagat
      481 ccgcacccac tacccgacga cgaccgagct tgagatcgac atcatggaac aggagcggat
      541 gacctacgag tcggacggcg tcaccgtcca gatgccgcag tacatgcggg agatcatcgc
      601 cgagatcacc catctggcgc ggcgcagcca cgacatcagc cagcgctcag gtgtctcggt
      661 ccgaatgtcg atcgccaatt acgagaacgt cgtcgccaat gcgctcaagc gcgccatccg
      721 caccaacgag aagcaggccg tcccacgcat ctccgacctg cgggcgatcg tcgcctcgac
      781 aaccggcaag atcgagctgg agacgatggg cgaggtccgc gaggatcgca tcgtcgagaa
      841 gctgatccag ggtgcgatcg tcacgacctt caaccgctac ttctcggtcc gcgactttga
      901 cgacctgatc ctcggcttcg ataacggcct cattgtcgac gccgccgacg atatgcccag
      961 catgaactac gtccaccagg tctcgcatat ggagggcgtc cgcgaggcga tcggcaagct
     1021 tgacatcgcc ggcagtccgg ccgggatcgc ctccggcgtc gagttcatcc tcgaagggct
     1081 gcacctgaac cggcgcctga acaaggacca ctcggcgtcg gccagcggct cacgctacaa
     1141 ggcataggag atcggcggga cgacgggccc tgctccggga catgttagac ttcaaggagt
     1201 cattttagtc aaacgacacg cgcggccgat cggctctgcg actcgtcacg caccaccggg
     1261 gcatccatgg cgcgccaaca tgtcgatacg accgaggacg accctgccaa tccggcgctt
     1321 ccctccgcca tcgtccgagt acagcttctg ggtggctttc gtctcgaggt cggttcccac
     1381 agcgtcgatg agttcaactg gcggctccgg aaagcccgga gcctggtcaa gctcctcgcg
     1441 ctggcgccca gacaccgcat gcatcgcgag caggttctcg atctactctg gcccgatcac
     1501 gatccggaag cggccggcaa caacctgcga agcacgctgc acgtcgctcg gcggatgttg
     1561 gagcagcacg ccggacccgt gccggccatc gcggtgcggg ctgactatct gttcctcttt
     1621 ccggatcatg acgtctggag tgatgtagag gcgttcgagg atgcggcaac ggccgcccgg
     1681 ctgacccgcg atccggatag ctttctgatc gcgacctcgc actaccgcgg tgagttactg
     1741 cccgaagatc gttacgagcc gtgggctgaa gaacggcgcg aacgcctgcg actcacgtgg
     1801 ctctcgttgc tacgcgattt gacggccctg tatgagcagc gcggcgagca gcgcagcgcg
     1861 atcgacgtgt tgctgcaaat gacgagcgcg gaaccggccg acgaagcggc ccacgtcgcg
     1921 ctgatgcgtc tctacgctca gaccggccaa cgactccagg cgctgcggca ataccagcat
     1981 ctgcgggatc agctgcaaca ggtgctggat gtcgagccgg agccggagag cacacggcta
     2041 tacgaagaca tcctcgccgg acggtttgac accatctcgc cagcgaccga gccaccggtg
     2101 accacgacgg tcattgacga ccatcgccca agtgcgccgc ccatcctcgt gccggttgag
     2161 ttgaccagct tcgttgggcg cgtgccggag ctcgatgaga tttgcgattt ggtgcggcgg
     2221 tcacggctgg tgacactgac cgggccgggt ggcagcggca agacgcgcct ggcgatcgcg
     2281 gccgcccgca ggcgggctga cgagtttgtt ggtggagtct ggttcgtcga tctcgccccc
     2341 ctcgccgacc cggcgctggg cgtcgccgag gtggccggaa ggcttggcgt cgctggtcag
     2401 gcagaccgct cgctgatcga cgatgtcggc gacgtcttgc gcgaccgttc cgcgttgatc
     2461 gtcctggaca actgcgagca tctcatcgat acctgcgccg ggctggccga tgcgctgctg
     2521 cgcgccgccg cgtcgctgac gatcctcgca acgagtcg
//
LOCUS       DASUGJ010000421         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_204264, whole genome shotgun sequence.
ACCESSION   DASUGJ010000421 DASUGJ010000000
VERSION     DASUGJ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204264"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1888
                     /locus_tag="VFV93_09265"
     CDS             <1..1888
                     /locus_tag="VFV93_09265"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5165572.1"
                     /translation="ITGSAATSEEAAQLSKADSHRADRISLPLTLIMLIMIFGGVVAA
                     VQPLFIGFLAAGVAIVMLGLFGRIFTVSNVAGTVTAVLGLGLSIDYALLIVTRFREEM
                     RDESSEVLEALKRTMNTAGRSVLFSGLAVATGMTSLAFVPLVAFRSLALAGCVTALFA
                     VVGALFILPAVLSIAGGNINRFNIFHLFGKRRTAEPNPDTDGGFFRRLAEFVVRHPLP
                     IAVISLIFLGILAIPLFRIRLGTSDYRILPESSNVREGYEVLVQAFGTGSAEPIKIAY
                     QDPNLLTPEGIGRLWDYVHQQVMTRPGIATGANNVPAVESAVSVLDSRLTNLSPQEQR
                     ALYVSLVPILARVQEVPASLPLPNGETMSRAELEAFVTLRDAIIKGDTALIQVSPSGD
                     PQSDEARDLVKALRDDRPASPATALIGGTPASSLDYVDEIVSAIPMVVIFVFILTYVV
                     LWALLGSVTLPLIAFVLNVVSLGASFGALVYIFQEGHFTGLLRFSGLGVLDATTPVVL
                     FAVTFGLSMDYQVFLLSRIREEYDRSGDVRTGIVGGLSHTAGIITGAAATLLVVLAAY
                     GTASNALVKSLTVGMFIAVLVDATVVRTFLVPSVLMLVGRPAWYSPKGLTRVWQRLGL
                     AERS"
     gene            complement(1883..>2386)
                     /locus_tag="VFV93_09270"
     CDS             complement(1883..>2386)
                     /locus_tag="VFV93_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5165573.1"
                     /translation="ALAEMRALIFELHPESLEREGLAAALERQVAALRARHGLVIECH
                     LDPVVDAPLHTQEALYRIAQEAMHNTVKHARAQHVDVRLVAGDRAIYLEVCDDGVGFD
                     PSGQFPGHLGHRSMRERAEGLGGTLTIASSMDRGTCVQVALPWTGIESAVAERSSQPA
                     SVNVPQS"
BASE COUNT          377 a          776 c          769 g          464 t
ORIGIN      
        1 catcaccggc agcgccgcaa cgtcggaaga ggcggcgcaa ctgagtaagg cggactcgca
       61 tcgcgccgac cggatctcgc tgccactgac cctgatcatg ctgatcatga tcttcggcgg
      121 tgtcgtcgct gcggttcagc cgctcttcat cggatttctc gcggcaggcg tggcgatcgt
      181 gatgctcggg cttttcggcc ggatcttcac cgtctcgaac gtcgccggca cggtgacggc
      241 cgtgctcggt ctgggactct cgatcgacta cgcgctactg atcgtcactc gttttcgaga
      301 agagatgcgg gatgagtcat cggaggtgct ggaagcgctg aagcgaacga tgaacacggc
      361 gggacggtcg gtcctcttca gcggcctcgc cgtagcgacc ggcatgacat cgctggcatt
      421 cgtcccgctc gttgcgttcc ggtcgctcgc gctggccgga tgcgtcaccg cgctgtttgc
      481 cgtggtgggg gcgttgttta tccttccggc ggtgctctcg atcgccggcg gcaacatcaa
      541 tcggttcaac atcttccacc tcttcggcaa acgccgaacg gccgaaccga atccggacac
      601 ggacggtggg ttcttccggc ggctggccga gttcgtcgtc cggcatccgc tgccgatagc
      661 ggtcatatcc ctcatcttcc tcgggattct ggcgatcccg ctgtttcgca tccgcctcgg
      721 gacgagcgac tatcgcatcc ttcccgaaag cagcaacgtc cgcgaaggat acgaagtgct
      781 ggtgcaggcg ttcggcaccg gctccgccga gccgatcaag atcgcctacc aggacccgaa
      841 cctgcttacg cccgagggca tcggccggct gtgggactac gtccaccagc aggtgatgac
      901 acggcccggc atcgccaccg gtgcgaacaa cgtgcccgcc gtcgagagcg ccgtctcggt
      961 gctcgactct cgactgacca acctgtcgcc acaggagcag cgggcgctgt acgtgtcgct
     1021 ggtgccgatc ctggcgcgcg tccaggaggt gccggcctcc ctgccgctgc caaacggcga
     1081 aacgatgagc cgcgccgaat tggaggcctt cgtcacacta cgcgacgcga tcatcaaggg
     1141 cgacaccgcg ctgatccagg tgtcgccgtc aggcgatccg cagtcggacg aagcacggga
     1201 tctggtgaaa gcactacgcg acgaccggcc ggcgtctccc gcgacggcgc tgatcggcgg
     1261 cacgccggcg tcatcgcttg actatgtaga cgaaatcgtg agtgccatcc cgatggtggt
     1321 gatcttcgtc ttcatcctca cctatgtcgt gctctgggcg ctgcttggct cggtgacgct
     1381 gccattgatc gccttcgtgc tcaacgtcgt ctcgctggga gcctccttcg gcgcgctggt
     1441 ctatatcttt caggaagggc acttcaccgg gctgttgcgc ttcagcgggc ttggggtact
     1501 cgatgcgacc acacccgtgg tcctcttcgc ggtcacgttc ggtctttcga tggattacca
     1561 ggtctttctg ctctcgcgga tcagagagga atacgatcgt tccggtgatg ttaggaccgg
     1621 catcgtcggc ggcttgtcgc acacggctgg gatcatcacc ggggccgccg caacactgct
     1681 cgtggtactg gccgcgtacg ggacggcatc gaacgcgctg gtcaaatcgc tgaccgtggg
     1741 catgttcatc gccgtgttgg tggacgcgac cgtggtccgc acgttcctcg tgccgtcggt
     1801 cctgatgctg gtcggccggc cggcgtggta ctcgccgaag ggcctgaccc gcgtctggca
     1861 acgactcggt ttggccgagc gatcatgatt ggggcacgtt gaccgacgcg ggctgactcg
     1921 aacgttcggc cacggctgat tcgataccag tccagggaag tgcaacctgc acgcacgtcc
     1981 cgcgatccat cgagctcgcg atcgtcagcg tgccgcccag accctcggca cgctcgcgca
     2041 tcgaccgatg gccgaggtgg ccgggaaatt ggccggacgg atcgaagccg accccatcgt
     2101 cgcagacttc caggtagata gcgcgatctc cggcaaccag tcgtacgtcg acgtgctgag
     2161 cgcgggcgtg tttcacggta ttgtgcatgg cctcctgggc gatccggtag agcgcctcct
     2221 gagtgtgcag cggcgcatcg accaccgggt ccaggtgaca ctcgatgacg agtccgtgcc
     2281 gcgctcggag ggccgcgacc tgccgctcga gcgcggcggc caaaccctcc cgttccagcg
     2341 actccgggtg cagttcgaag atcagggcgc gcatctcggc cagggc
//
LOCUS       DASUGJ010000422         7060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_343263, whole genome shotgun sequence.
ACCESSION   DASUGJ010000422 DASUGJ010000000
VERSION     DASUGJ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343263"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFV93_09275"
     CDS             complement(<1..334)
                     /locus_tag="VFV93_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165574.1"
                     /translation="MDTNLARTSSAPLTLAETTAAHRRHDWAQLLSDPAVVFGIVVFL
                     IVGISAIAAPVIAPQDPALQSLSDRLQAPTIFDGSTKHLLGTDNLGRDILSRLIYGAR
                     VSLIVAVVT"
     gene            complement(334..1341)
                     /locus_tag="VFV93_09280"
     CDS             complement(334..1341)
                     /locus_tag="VFV93_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5165575.1"
                     /translation="MSAYILKRLIHLIPVLFGVSVIVFVLMRVVPGDPISVYTQDQLL
                     PEESIQQLRKSLGLDLPLPQQYVRYMGQLLRGDFGESLQYDQPVLSLIGDRLPATIEL
                     SIAALLLSLLLAIPIGILAAVFRGSIFDRIAIIISVLGVSLPTFWVGILLIYGVSLHL
                     GWFSEFGRIDIGTTVPHVTGLYTVDSLLAGDFGAFRSALSHLVLPAVSIALSMQAFTL
                     RLLRSSTIEALMSDYVVAARARGLSERAVVLRHAVRNALLPTITVIGLQIGGLVGGVI
                     ITETIFSWPGIGLLIVQAINSRDFVLVQGIVVVFAVVYVVLNLLTDLAYAWADPRIRL
                     S"
     gene            complement(1432..3192)
                     /locus_tag="VFV93_09285"
     CDS             complement(1432..3192)
                     /locus_tag="VFV93_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5165576.1"
                     /translation="MGDVNETPAILERLQVGWSRRTFMKRAALFGLAVPAMSGLLAAC
                     GDSDDDDDDDTGQSGSATQTPSTQAPTATTAAGGATTTVGGAATADTGSATTEATSAP
                     EETPTAADDEGSLDSQLVIAQVSDLQALYPGVYTSRQSANIMLQVYEGLVLREDDMEF
                     IPGLAESWERVDNTTWRFNLRSDVTFQNGKPFVAEDVKLSFEEGANHPETGVYRYRSF
                     LQEVKVIDDTTIDVITSIPYSPLLTKIVNMEVMNAETYTEVGVEGLSEQPVGTGPFRF
                     MEWKQGERVVVEAYEDYWGGRPKLGTVVFVPIAENATRTAALESGTVQIAAEVPAEYA
                     KTPREGVEIATVDGTRLFFVGLNVEFEPLSDPKARQAMNYGVNVPEIVEALLDGKAEV
                     LHQPAFKKMFGYSDKIEGYHYDPDQAKQLLAEAGLEDGFSATLDVAPTFKEIAEAIAG
                     QLSAINVSIEVNVMDPQALTEKYNSGASEMYFSSWGVSELDPDNPYSNHFLSTRPQVY
                     THYSRPDVDDLITRAAGEVDDTERLRLYEEVTQIIVDDAPWIFLYTPAEIYGVRTSVS
                     NWNPRSDSRFNLMETTVAAE"
     gene            complement(3269..5050)
                     /gene="ggt"
                     /locus_tag="VFV93_09290"
     CDS             complement(3269..5050)
                     /gene="ggt"
                     /locus_tag="VFV93_09290"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5165577.1"
                     /translation="MMTKEEGVSEVVRRSVLVQQKVEVTSDAGLVAAESEEAAEAGAE
                     IFEAGGNAIDAAVATSFTAAVCEPAMTSIGGGGAALVYLGETGETFALEFNGRLPHAA
                     HENMFLDDLLPPGESLSPFSWRGTRDNVAWMGYRTLGVPGHPAGVLSLHERFGTLPRS
                     TVLAPAIRRAREGYDINPYYSLLIGHDAKHILRFPSLAHMLLPDGRPPVPRDYYNPNG
                     HRVVQPQLAETLEMIAEGGIDAFYRGDVARAIVEDNRPHGAIVTLEDYAAYEPRWYDD
                     GLRTDYRGYTVVAMPDAFGGVNVLETLNILERFNLARSGHNTPQTLHLLVEALRLSWS
                     DRFRHMGDPEFEDVPIRGLISKAYADERRGLIDARQAPDEIRPGDPWPYQGMRAPANE
                     KPGGTHPPGNETTHMVTADAQGNMVSLVQTLGGEFGSRTISPSTGIIQRNYTNLFNPE
                     PGTKNSIGPWKRPLSHDSLVLILKNGRAIAAVGAPGGRRVIPAVIQVILNIIDFGMGI
                     QDAIGAPRIHAEGCDPKVPEGLLVRTVFVDSRIPAETRAAMEAMGHEIVVMQDGQFAL
                     PVGLLRDEAGLLHGGVTVPVPAMAIGL"
     gene            complement(5073..5969)
                     /locus_tag="VFV93_09295"
     CDS             complement(5073..5969)
                     /locus_tag="VFV93_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX5165578.1"
                     /translation="MSSELQRRIAERHDQLSPKRRRIARFIADNETFIAFASASEVGK
                     AVNVDTATVVRLAQSLGFNGYPDLQEAIRSQLPERLTFVQRVRQQQTQPRDEQFLPVQ
                     VFTNDIENLNQTMNQTDPDVVRAVVNAIRQADHVFVIGSGFSAATAVFLGHGLSSAGI
                     RTTVCTEGGSSLIVKLATVRANDLVVGVSVWRYLREIVDAVARAAERGTPTVALTDSY
                     VSPLARIADHSLIASTRGVSHSLSPVGLMALVNLLLAEIDAVEPERSLQSLQEIDQLY
                     RQRGMVDGEQRDQPDGQVNGTD"
     gene            complement(6237..6662)
                     /locus_tag="VFV93_09300"
     CDS             complement(6237..6662)
                     /locus_tag="VFV93_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5165579.1"
                     /translation="MEAHSQRAQLRALPQDGGDLPPVVADLLRQLLEQLDEHALAGSA
                     HASGVEGQDILLDIRMDGHSYTLARTADTTQRDGISLSPRETEIVRLVARGLPTKAIA
                     NVLDVSLWTVSTHLRRVFAKLQVNSRAEMVARALAEGLI"
BASE COUNT         1300 a         2212 c         2255 g         1293 t
ORIGIN      
        1 cggtgacgac cgcaacgatg agcgagacgc gcgcgccgta gatcagccgc gacaggatat
       61 cgcgccccag gttgtcggtc ccaagcagat gcttcgtgga cccgtcaaag atggttggag
      121 cctggagccg atcgctcaac gattgcagcg cgggatcttg cggggcgatg acgggcgcgg
      181 cgatcgcgct gatcccgaca atcaggaaga cgacgattcc gaacacgacg gccggatccg
      241 acagcagctg ggcccagtca tgccgcctgt gggcagcggt tgtctccgcc agcgtcagtg
      301 gagcggagga tgtcctcgcc aggttcgtat ccatcagctt aaccggattc gcgggtcagc
      361 ccaggcatag gccagatcgg tgaggagatt cagcacgacg tacacgacag cgaagacgac
      421 gacgatgccc tgtaccagca cgaagtcgcg cgagttgatg gcctgcacga tcagcaggcc
      481 gatccccggc cacgagaaga tcgtctcggt gatgatgacg ccaccaacca atccgccgat
      541 ctggaggccg atgacggtga tcgtcggaag cagggcgtta cggacagcgt gccgcaggac
      601 gaccgcgcgc tccgagaggc cgcgcgcccg cgcggcgacg acgtagtccg acatgagcgc
      661 ctcgatcgtg ctgctgcgca gcaaccgtaa tgtgaacgcc tgcattgaga gagcgatcga
      721 gacggccggc aggaccaggt gggagagcgc gctcctgaag gcgccgaagt cccccgcaag
      781 caggctgtcg accgtgtaga gcccagtcac gtgcggcacg gtcgtgccga tgtcgatgcg
      841 gccgaattcc gagaaccagc ccaggtggag tgacacaccg tagatcagca ggatgccaac
      901 ccagaaggtc ggtagcgata cacccaggac cgagatgatg atggcgattc ggtcgaagat
      961 gctgccccgg aagacggcag ccaagatccc gatcgggatc gccagcaaga gtgacagcag
     1021 caaggcagca atactcaatt cgatggttgc cggaagccga tcgccgatca gcgaaagcac
     1081 cggctggtcg tattgaagcg attcgccgaa atctccccgc aggagctgcc ccatgtagcg
     1141 cacgtactgt tgcggcaacg gcagatccag cccaagcgac ttgcgcagct gctggatcga
     1201 ctcttccggc aggagctggt cctgggtata gacgctgatc ggatcacccg gcacgacccg
     1261 catcagcacg aacacgatca ccgaaacccc gaacagaacc gggatcaggt ggatcagccg
     1321 cttgaggata tacgctgaca ctacagctct acctctctga gggagcgtga atcatgtatc
     1381 cggatggccg atccaccgtc accggctagc cctcgccgga tcacctgctt gctattcggc
     1441 tgcaaccgtc gtttccatca ggttgaaccg cgaatccgat cgtgggttcc agttgctgac
     1501 cgatgtccgg acgccgtaga tctcggccgg ggtatagagg aagatccacg gcgcgtcatc
     1561 gacgatgatc tgggtcactt cctcgtagag gcgcagtcgc tctgtatcat caacctcccc
     1621 ggcggccctg gtgatcaggt catccacgtc cggacgcgaa taatgcgtat agacttgggg
     1681 ccgggttgac aggaagtgat tgctgtaggg gttgtccggg tcgagctcgg acacacccca
     1741 ggacgagaag tacatctcgc tggcgcccga gttgtacttc tcagtcagcg cctggggatc
     1801 catgacgttc acctcgatgc tgacattgat cgccgaaagc tggccggcaa tcgcctcggc
     1861 gatctccttg aaggtcgggg cgacatcgag tgttgccgag aagccatcct ccagacctgc
     1921 ctcagccagc aactgcttcg cctgatcggg atcgtagtgg tagccctcga tcttgtccga
     1981 atagccgaac atcttcttga acgccggctg gtggagcacc tcggccttgc catcgaggag
     2041 cgcctcgacg atctcaggaa cgttgacgcc gtagttcatc gcctggcgag ccttcggatc
     2101 ggagagcggc tcgaactcca cgttgaggcc aacaaagaag agccgtgtcc catccacggt
     2161 cgcgatctcg acgccttcgc gcggtgtctt ggcgtattca gcagggacct cggcggcaat
     2221 ctggaccgtg cccgactcga gcgcagccgt ccgggtcgcg ttctcggcga tcggcacgaa
     2281 gacgactgtg ccgagcttgg gccggccacc ccagtagtct tcgtacgcct cgacgacgac
     2341 ccgttcgccc tgcttccact ccatgaagcg gaacggcccg gtgccgaccg gctgctcgga
     2401 caacccctca acgccgactt cggtgtaggt ctccgcgttc atgacctcca tgttgacgat
     2461 cttcgtcagc aagggcgagt aggggatcga tgtgatgaca tcgatggtcg tgtcatcgat
     2521 gaccttgacc tcctggagga aggagcgata ccgatagacg cccgtctcgg gatggttcgc
     2581 gccttcttcg aagctgagct tcacgtcttc ggcaacgaac ggtttgccgt tctggaacgt
     2641 aacatcgctg cgcaggttga atcgccaggt cgtgttgtcc acccgttccc atgattccgc
     2701 cagacccggg atgaactcca tgtcgtcctc gcggaggacg agtccctcgt agacctggag
     2761 catgatgttc gcgctctggc gggaggtgta gacgccggga tagagtgcct ggaggtcgga
     2821 cacctgcgcg atgacaagct gtgaatcgag tgacccctcg tcgtcagcag cggttggcgt
     2881 ctcctcaggc gcgctggttg cttccgtcgt cgcgctgccg gtatctgccg tcgctgcgcc
     2941 gccgactgtc gtcgttgcgc caccagctgc cgtcgttgcg gtcggcgcct gcgtcgacgg
     3001 cgtttgtgtc gccgatccgg actggccagt gtcatcgtca tcatcgtcgt ctgaatctcc
     3061 acaggcagcc agcaggccgc tcatggccgg gacggcgaga ccgaagagcg ccgctcgctt
     3121 catgaacgtt cggcgggacc atccgacctg gagtcgctcc aggattgctg gagtctcatt
     3181 cacgtcaccc atgtcactca ctccttcacg tgctggtttg tgaacatcgc atcgctcgac
     3241 gtgcccgaca ccgaatcgac cgtatcccct acaggccaat cgccattgcc ggaaccggga
     3301 cagttacccc gccatgcagc aaccccgcct cgtcgcggag gagaccgacc gggagcgcga
     3361 actgcccgtc ctgcatcacg acaatctcgt ggcccattgc ctccattgca gcccgggttt
     3421 ccgcagggat ccgcgagtca acgaacacgg tccggacgag aaggccctcc gggaccttcg
     3481 ggtcgcagcc ttcggcgtgg atgcgcggcg cgccgatcgc atcctggatg cccatcccga
     3541 agtcgatgat gttgaggatg acctgaatca cggctgggat gacccgccgg ccgcccggag
     3601 cgccgacggc tgcgatcgca cggccgttct tcaggatcag gacgagtgag tcgtggctga
     3661 gcggccgctt ccacgggccg atcgagttct tcgtacccgg ctccgggttg aacaggttgg
     3721 tgtaattgcg ctggatgatg ccggtgctag gtgagatcgt tcgcgacccg aactcgccgc
     3781 ccaacgtctg gaccaggctg accatgttgc cctgcgcatc ggctgtcacc atgtgggtgg
     3841 tctcgttgcc cggcgggtgc gtcccgcccg gcttctcgtt agccggagcg cgcatgccct
     3901 ggtacggcca cgggtcaccg ggccggatct catccggcgc ctgccgagca tcgatgagac
     3961 cccgccgttc gtcggcatac gccttcgaaa tcaggcctcg gatcgggaca tcttcaaact
     4021 ccgggtcgcc catgtggcgg aagcggtcag accaggagag tcgcagcgcc tcgacgagca
     4081 ggtgcagggt ctgcggggtg ttgtggccgg atcgagccag gttgaacctc tccaggatat
     4141 tcagcgtctc caggacgttg acgccgccga acgcatccgg catcgcgacg accgtatagc
     4201 cccggtagtc ggtgcgcaga ccatcgtcat accagcgtgg ctcgtaggcg gcgtagtctt
     4261 ccagggtgac gattgcgccg tgggggcggt tgtcctcgac gatcgccctg gcaacgtcac
     4321 cacgatagaa ggcgtcgatg ccgccctcgg cgatcatctc aagtgtttcc gcaagctgtg
     4381 gctgcacgac ccggtgaccg tttgggttgt agtaatcgcg tgggacgggt ggacgaccgt
     4441 cgggcaggag catgtgcgcc agcgagggaa agcggaggat gtgcttcgcg tcgtgaccga
     4501 tgagaagcga atagtatggg ttgatgtcgt atccctcgcg ggcgcggcgg atggcaggcg
     4561 ccaggaccgt cgagcgtggc aacgtgccga accgctcgtg caacgagagc acgccggctg
     4621 gatggcccgg cacgcccaac gtgcggtatc ccatccaggc cacgttgtca cgcgtcccgc
     4681 gccagctgaa cggactgagg gactcgccgg gcggcaggag gtcatcgagg aacatgttct
     4741 cgtgcgcggc atggggcaac cggccattga attcgagggc aaacgtttcg cctgtctcgc
     4801 caagatagac gagggccgcc ccgccaccac cgatactggt catggccggc tcgcagaccg
     4861 cggccgtgaa cgatgttgcg accgccgcgt caatcgcgtt cccgcccgcc tcaaagatct
     4921 cggctccggc ctcggcggct tcctcgctct cggccgcgac caggccagcg tcggaggtca
     4981 cctcgacctt ctgctggacc aacacactgc ggcgtaccac ctcgctcacc ccttcctctt
     5041 tcgtcatcat tgagtctcgt tcgcgtagtg ccctaatcgg tcccgttcac ctgaccatcc
     5101 ggctgatcgc gctgctcacc atccaccatt ccgcgctggc gatagagctg atcgatctcc
     5161 tgaagcgact ggagcgagcg ctcaggttcg acggcgtcga tttccgcgag caggagattg
     5221 acgagtgcca tcagcccgac cggggacagg ctgtgactga cgccccgcgt tgatgcaatc
     5281 agcgaatggt cggcgatccg cgcgagtgga gagacgtagc tatcagtcaa cgcgacggtc
     5341 ggcgtgccac gctcggctgc ccgggcaact gcgtcgacga tctcccgcaa gtagcgccag
     5401 acactgacgc cgacgacgag gtcgttcgcc cggacggtcg ccagcttgac gatcagactg
     5461 gagccacctt cggtgcagac agtcgtgcga atcccggcgc tgctcaggcc atggcccagg
     5521 aagacggccg tagcggccga gaagccggag ccgatgacga agacgtgatc tgcctggcga
     5581 atcgcgttga cgacggctcg caccacatcc gggtcggtct ggttcatcgt ctggttgagg
     5641 ttttcgatgt cgttcgtgaa gacctggacc ggaagaaact gctcgtcgcg tggttgcgtc
     5701 tgctgctgtc gcacacgctg gacaaacgtc aagcgctccg gcaactggct tcgaatcgcc
     5761 tcctggagat cgggatagcc gttgaagccg agcgactgcg ccaggcggac gaccgtcgcc
     5821 gtatcgacgt tgacggcttt gccgacctca ctggccgagg caaaggcgat gaacgtctcg
     5881 ttgtcggcga tgaagcgggc gatgcgccgc cgcttcggac tgagttgatc gtgccgttcg
     5941 gcgattcgcc gttggagttc ggaggacacg gttgaactcc taccgtctgc aacgccaatg
     6001 ggttcgtctg ttcagtgctt aaccgcgatc agtctagcac ggcaaatgcc gaagtgcaac
     6061 agatgtggcg atctgaggtg taattgcaat gagtatggcg tgctttcggc aaacccgatc
     6121 gaacggcctg catgaagagc agcaacggcc ggcgccgcga cacgcatgag tcgcgttcga
     6181 acgtctgatg gagcgaaaac ggaggtgttg gggcaaccct gtcgggtgat ctcgtgtcag
     6241 atcagtccct cggcgagggc gcgggcgacc atctcggccc gtgagttgac ctgcagcttg
     6301 gcgaagacgc ggcgcaggtg ggtcgagacg gtccagaggc tgacatcgag cacgttggcg
     6361 atcgccttgg tcggcagacc gcgcgccacg agccgcacga tctccgtctc gcgcgggctg
     6421 aggctgattc cgtcgcgttg ggtggtgtcg gccgtccgcg ccagcgtgta ggagtgcccg
     6481 tccatgcgga tatcgagcag gatgtcctgc ccctccacgc cggaggcatg cgccgagcca
     6541 gccagcgcgt gctcgtcaag ttgttcaagt agctggcgca gcagatccgc cacgaccggg
     6601 ggcagatcgc cgccgtcctg aggaagcgcg cgaagctggg cgcgctgtga gtgtgcttcc
     6661 accgcttctc ctccttgttc cctcggaggt gatgaagcga gcccgccggt cgcacggtca
     6721 gccggatcga tgaccccggc gaccgttgac agccgctgcc acggctcacg gtgggacacc
     6781 gaaatgatcc cgctgccacg accggcatcg cacactggcc cgtacgacga cgcgcagcat
     6841 cgattgcatt gtgtttgcat tatgcgtcta tagtgcatca gcattacaac atgtggcgct
     6901 acagtagcag atactcacgc atatcacaat ccatctacca cattcgtgtc cgtcgcctgt
     6961 ttgagtgacg cacatccatg ggaaccgctc cgtgtgtcgc tcatagcggg gactgtcgca
     7021 ggtggccggc cgtggctagg ctgggcgcca cagtgacctc
//
LOCUS       DASUGJ010000423         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2010749, whole genome shotgun sequence.
ACCESSION   DASUGJ010000423 DASUGJ010000000
VERSION     DASUGJ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010749"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(474..1664)
                     /locus_tag="VFV93_09305"
     CDS             complement(474..1664)
                     /locus_tag="VFV93_09305"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX5165580.1"
                     /translation="MSSRRTASLTPDEARQLHQQALVIDTQQPPIMTGIVFTPGMRAA
                     VAELAQQGRTRAEAAPVLEQVLTKEIQSSPEAREIYLDMWRRSGVTVACGTFSGFDRL
                     ATAFESAVRKIANAHEMVNALKEQMLIVRTAADIETAHAENRHGVVIDFQNTIAIGDD
                     LDRIDLFHGLGLRMVQLTYNLQNLVGDGCTETYQSGLTWFGRDMVKRLNDARILVDVS
                     HCSEQVGWDAMEVSTAPVIVSHSSSAAVAKHDRGKSDKLAKAVADQGGYFGVVAIPGF
                     IRETPGASIDDVVRQIEHLVNVCGIDHVGIGTDKAGPGPRTSSMVEFPDSMPAGLPGD
                     FNWSGFRIQEHRQTPEYQMEGFESLADWPNITAGLAQAGFNEDELRKLLGLNYLRVFR
                     DVVG"
     gene            1770..>2702
                     /locus_tag="VFV93_09310"
     CDS             1770..>2702
                     /locus_tag="VFV93_09310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5165581.1"
                     /translation="MTVSLWQDTAAWPGEVEHERIVADIAVVGGGIVGATLATFLAAA
                     GRDVVVLEAGHVASGASGRNAGHCLAGMRHNYHQMVERLGRERAKALRTQLLENREMV
                     HRLIQQFDVPHERPGSQYLGVDAQDGAELAACVRAFHADGFDDIEYSETDPYGRGFFG
                     RLYQPGDMWLQPYLLVTRMMASSGARVIENCPVRAIEQVGDTVTIRGRIATVECRQAV
                     LATNAYSRTLHEYFRDKVTPCRGQMYATEPSTEPRLIEMPTGTDRSTGDNSAYFRQLP
                     DGRFLIGGFRRAFIDEELGYGDETTPQLQATMQGW"
BASE COUNT          452 a          866 c          913 g          471 t
ORIGIN      
        1 cgctctcccg atgaatctgc gccggttgag cgttgaatcg accagttcgg tgacgcgttc
       61 cagggacaac tcgtgctgaa ccatgcctgc tctcctccac gtgtgcccac gccagtcccg
      121 tgcataccat tagcggctct ctcaaccgcc gggaccggat cggctgctgg acaacgagac
      181 gaccgcgctc aagcgcattg cgcattgcgc atagacttgc tctccacagc tcctttcatg
      241 taggtccgtg cgaatcggtt caaatcagtc atcggcgccg cacacggatg atccgctgcg
      301 gcggcaacaa acgcgatcaa cgcggggacg cgatttgcac cacgagcaat gtggacgagc
      361 aaaccgggca atgcagcaat gaaacgaccg aggaaacagg gcaacattgc ccttgacacg
      421 ccatactacg gggcgacgct gatcccgtca atgttgcgca ggtcgtgacg cgcctacccg
      481 accacgtcgc ggaacacgcg caggtagttg aggccgagca gcttgcgtag ttcgtcttcg
      541 ttgaagccgg cctgggcgag gccggcggtg atgttgggcc agtcggccag gctctcgaag
      601 ccctccatct ggtactcggg cgtctggcgg tgctcctgga tgcggaagcc ggaccagttg
      661 aagtcgcccg gcaggccggc tggcatcgag tcggggaact cgaccatcga gctggtgcgc
      721 gggccaggac cggccttgtc ggtgccgatc ccgacatggt cgataccgca gacgttgacg
      781 aggtgctcga tctggcggac gacatcgtcg atcgacgcgc cgggtgtctc gcggatgaag
      841 ccggggatgg cgacgacacc gaagtagccg ccctggtcgg cgacggcctt ggccagcttg
      901 tccgacttgc cgcgatcgtg cttggcgacg gccgcgctcg atgagtgcga gacgatgacc
      961 ggcgcggtcg agacctccat cgcgtcccag ccgacctgct cgctacagtg gctgacatcg
     1021 accagaatcc gggcgtcgtt cagccgcttg accatgtcgc ggccgaacca ggtcagcccc
     1081 gactggtagg tctcggtgca accatcgccg accaggttct gcaggttgta ggtgagctgg
     1141 accatccgca gccccaggcc gtggaagaga tcgatccggt cgagatcgtc gccgatcgcg
     1201 atcgtgttct ggaagtcgat gacgacgccg tggcggttct ctgcgtgggc cgtctcgatg
     1261 tcggcggctg tccggacgat cagcatctgc tccttgagcg cgttgaccat ctcgtgggcg
     1321 ttggcgatct tgcggacggc gctctcgaag gccgtcgcca gccggtcgaa gccggagaag
     1381 gtgccgcagg cgacggtgac acccgaccgt cgccacatgt cgaggtagat ctcacgcgct
     1441 tccggcgagc tctggatctc ctttgtcagt acctgctcga ggaccggcgc cgcctcggca
     1501 cgtgtccgcc cctgctgcgc cagttcggcg acggccgccc gcatgccggg ggtgaagacg
     1561 atcccggtca tgatcggtgg ctgctgggtg tcgatgacga gtgcctgctg gtgaagctgg
     1621 cgggcttcgt caggcgtcag gcttgcggtg cgccggctag acattgggtg gctcctctcc
     1681 ccatgggaaa cagttcgcgc ggctatcata gcagcggttt gtcagttggc agttcagcgt
     1741 gcgtagcgcc cgattggtga ggagcaacaa tgaccgtctc actctggcag gacacagccg
     1801 cctggcccgg ggaagtcgaa cacgagcgga tcgtcgctga catcgccgtt gtcggcggcg
     1861 ggatcgttgg cgcgacgctg gcgacgttcc tcgctgccgc cgggagggat gtcgtcgtcc
     1921 tcgaagccgg tcacgtcgcc agcggagcca gcgggcgcaa cgccggtcac tgtctggccg
     1981 ggatgcggca caactaccat cagatggtcg agcggctggg gcgcgagcgc gcgaaggcgc
     2041 tgcggacgca actgctggag aatcgcgaga tggtgcatcg cctcatccag cagttcgacg
     2101 tgccacacga gcggcccggc tcccagtacc tgggcgtcga tgcgcaggat ggggcggaac
     2161 tggccgcctg cgtccgggcg ttccacgccg acggcttcga cgacatcgag tacagcgaga
     2221 ccgacccgta cggccgtggc ttcttcggcc gcctctacca gcccggcgac atgtggctcc
     2281 agccttatct gctggtgacc cggatgatgg cctcctccgg cgcgcgggtg atcgagaact
     2341 gtcccgttcg tgcgatcgag caggtgggtg acaccgtcac gatccgtgga cggatcgcaa
     2401 ccgtcgagtg tcgccaggcg gtgctggcga ccaacgccta ctcgcggacg ctgcacgagt
     2461 atttccgcga caaggtcacg ccctgccggg ggcagatgta cgcgaccgag ccgagcacag
     2521 agccgcggct gatcgagatg ccgaccggca ccgaccgctc gaccggcgac aactccgcct
     2581 acttccggca gctccccgat ggccggttcc tgatcggtgg cttccgccgc gccttcatcg
     2641 acgaggagct gggctacggt gacgagacga cgccgcagct tcaggcgacg atgcagggct
     2701 gg
//
LOCUS       DASUGJ010000424        17283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1453478, whole genome shotgun sequence.
ACCESSION   DASUGJ010000424 DASUGJ010000000
VERSION     DASUGJ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 17283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17283
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453478"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFV93_09315"
     CDS             <1..498
                     /locus_tag="VFV93_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEX5165582.1"
                     /translation="LSGLIFMLLIISQFIAYFNFSNMPTVIAGGMAHLLESANVHELL
                     LLIGLIVIIAVLDIIIPGAMPKWAIFAPIFVPLFIRLGVAPQTVLAAYRIGDSPMNVV
                     TPLMVYLPFIVIVSQRYVKESGIGTIVSLMIPYTLVVLAIWIIFFVIWYAVGVPLGPG
                     YGVGT"
     gene            546..1319
                     /locus_tag="VFV93_09320"
     CDS             546..1319
                     /locus_tag="VFV93_09320"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX5165583.1"
                     /translation="MKSGIWLVGILAASMLLAGCGGEASADSLTGTKWELDELNGRDA
                     LDDVLVTMQLDDDDDLSGTSGCNRYIGSWETDDGDKITLEPSGLTMMACDQPVMDQEQ
                     AFLEALASAVRFDLGVDELELYNAEGREVAEFDRLKPINLMRTPWVVVAINDGENRAV
                     PVLEGTTLMALFESEDDRLSGQGGCNTFTTTYSRDGEEMTIETPIAATLMACDQPVMD
                     QETMFFEALGRTATFELGDETLLLRSEDGSMQVWFKDAD"
     gene            1429..2070
                     /locus_tag="VFV93_09325"
     CDS             1429..2070
                     /locus_tag="VFV93_09325"
                     /inference="COORDINATES: protein motif:HMM:NF025255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HEX5165584.1"
                     /translation="MFADILVGLALGAFGLFVCFMGLRLFFFALPIVGFVTGFYIGVA
                     GTRAVLHEIFLSTVTAVIIGLIVGVALGILAYFLWYVGALISAGTTGALIGSGLMHTF
                     GVTSGWVVFLVALAAGIFLFLVAFKLALPIWVVIVNSAMLGAAGAVAGVMLIFDQIDR
                     SDLNYGLAWAAIEDSWFWIIAWAIVAVIGIGMQSQAIEKTTLPQDRWGAAHAT"
     gene            2184..3350
                     /locus_tag="VFV93_09330"
     CDS             2184..3350
                     /locus_tag="VFV93_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165585.1"
                     /translation="MDDQRPNNTDPGQEPENLDNEAVGGTSEPAGEDIAPDAQPTQPL
                     ATPPSTPPGAPPPSDNDGRQRWIIGGVLAFVLVAALLALLLFRDDDDGDDTEAEEQAT
                     EEVATETAMAAIEPTATVVEPTATSEPEPTETEVQATETPEPDDEETEVAATETAEAE
                     PEPTETKTAPTETAEAAEPTATKTPEEAKPTEEPKPTATEEEPEPTPDDSDLAYEADW
                     STGNNGWVLTSGWAIENGNLVTSGNAAPLTAPFESQRADYAVEMTMTVTDLDDCDARV
                     GVFARVTDAAGPSGTVHVGYPASVCDDAWHIDSVLADDRDSLASGDRPLASGSHDYRI
                     EVAGERIRLYIDDEFVGAATDNRWTEAGRGGIYVDGDAQVQISAFRIFLLSDTP"
     gene            3347..3790
                     /locus_tag="VFV93_09335"
     CDS             3347..3790
                     /locus_tag="VFV93_09335"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX5165586.1"
                     /translation="MTGKAHTEGGFKLERLVTHAAAGATVGSLWGALIGLVFLMPAAG
                     AIVGAASGAVAGTFVGVVRTDDANNHGFGQFGQQFSAVMVLGSSAVLMLVYTKDPDGA
                     IATLKQYSGRVLRSTLPDQVEAQLHAALSQLPAKRLLHPDQLCRR"
     gene            complement(3841..3913)
                     /locus_tag="VFV93_09340"
     tRNA            complement(3841..3913)
                     /locus_tag="VFV93_09340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3878..3880),aa:Gly,seq:tcc)
     gene            complement(3926..3998)
                     /locus_tag="VFV93_09345"
     tRNA            complement(3926..3998)
                     /locus_tag="VFV93_09345"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3963..3965),aa:Pro,seq:tgg)
     gene            complement(4039..5247)
                     /gene="tyrS"
                     /locus_tag="VFV93_09350"
     CDS             complement(4039..5247)
                     /gene="tyrS"
                     /locus_tag="VFV93_09350"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX5165587.1"
                     /translation="MMHTNRDRLIADLLSDARVALIQVREHLERRLRTGDSLRVKFGV
                     DPSRPDLHIGHYVAFRKLRQFQQLGHTVVIIIGDWTAQIGDPTGRSTQRTMLTAEEVR
                     NNARTYLDQLFRVIDVERAEIRHQSEWFGGFDLADVLRLTARYSVAQMLQREDFQKRI
                     AENTSIGIVELLYPLLQAYDSVAVAADVEIGGTDQTFNLLVGRDIQRASGQPPQDVLT
                     VPLLVGLDGAMKMSKSADNYVGVAEVPVTSFGKLMSIPDRLIEQYYRLVTSVAGDDID
                     AIVQAMHTGATNPRDVKAILAHTIVADLHGQEAADFAQAEFERVFRDGATPSEIADTL
                     VDLPATIQAILVSTRLATSNNDARRLVEQGGVRLNNEVIDSVTTLISLNEPAILRVGR
                     RRFLRLVPRN"
     gene            complement(5247..6215)
                     /locus_tag="VFV93_09355"
     CDS             complement(5247..6215)
                     /locus_tag="VFV93_09355"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX5165588.1"
                     /translation="MTQVPEGAIATLERIARVPHVMTIGNFDGVHRGHQYLIQRVIDA
                     AVARGSRSLVVTFEPHPTAVLRPDVPFHRLASPERKLEILNAAGVDDIAVIPFDREFA
                     ALEPEEFLELLARTVQPVAVFVGSGFRFGKGRAGDGGMIEAFGQTHGFETTVVERLQD
                     GDVTISSSSIRDALSRGNIARATASLGRRYRLRGTVEHGAARGRELGFPTANLLLPAD
                     ACVPADGIYAGYAHLSDRALGPRAAMIYIGTRPTFDNGDRQVEVNILDFSGDLYTLDL
                     EIEFVAWVRGDQAFDSAEALAAQMQDDEATSRAILASNQPEPANPD"
     gene            complement(6212..7144)
                     /gene="truB"
                     /locus_tag="VFV93_09360"
     CDS             complement(6212..7144)
                     /gene="truB"
                     /locus_tag="VFV93_09360"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX5165589.1"
                     /translation="MTRSKLPDLNGLLVIDKPAGMTSHDVVGRVRRLTGMKRVGHGGT
                     LDPFATGILVVAVGRATRLLQYVQDSQKGYRAHVVLGASTDTADVDGVLNEIGDGGGW
                     PGHEEVTTTVARFVGEIEQVPPAYSAIKVGGRKLYELARAGEDVEVPTRMVTIHTIDT
                     VSYRPPDLILDIHCGKGTYVRSIARDIGVALGTEAYCHGLRRTNTGRFCLADAWTLDE
                     LTERDVRQEWPQIALHPDAALADLPAVVLGEGDQAAWYHGRSVQLSPASQSANAPVRA
                     YGDDGRFLGIGTRTADNALKPSLVLPPEDEGEES"
     gene            complement(7131..8222)
                     /locus_tag="VFV93_09365"
     CDS             complement(7131..8222)
                     /locus_tag="VFV93_09365"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX5165590.1"
                     /translation="MIDSFQENGSEPAANGHETLRSIPAAAHAIRDAVRGSSRPVILT
                     HANPDADAVAAALGMRTLVQSFGVEPVLTSSGDAALPSNLEFLVGALDLKHAADEAVA
                     EADLLILVDCSDPSRLGPLFYRMATEFERTRPIINVDHHVTNHRFGTLNLVVPAAAST
                     AEIVVGLFDELGVEIDADTATTLLAGIYGDTLGLRTPSTTPASLRTAAELIEAGADMD
                     SIVDALFRLKPYSTICLWAEALQRAQWRGALMWTQIDPEMLERSGADRTEAEGIVNFL
                     AGTIGARASALLYRESWGWRVSMRSLADDVDVAKLLGIFGGGGHPRAAGARLDAGEDA
                     KERFLNEVAGLLGPRGALTGRASGGDDPV"
     gene            complement(8219..8623)
                     /gene="rbfA"
                     /locus_tag="VFV93_09370"
     CDS             complement(8219..8623)
                     /gene="rbfA"
                     /locus_tag="VFV93_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX5165591.1"
                     /translation="MSGRRRERVGDLLRDEVSEIIQREMSDPRLGFVSITRVELSPDL
                     KYARMFVSVMGSEDERASSLVALNNASGFIRRQLAPRLRMRVIPEVSFRLDRSMEHAE
                     NVARILRDLEPELRAAEAKENEPASTDEGTPA"
     gene            complement(8708..10621)
                     /gene="infB"
                     /locus_tag="VFV93_09375"
     CDS             complement(8708..10621)
                     /gene="infB"
                     /locus_tag="VFV93_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5165592.1"
                     /translation="MSGQFRRPGGGGSNRNRRPGGGRRGGPRGDHGPRNRGGNTATIQ
                     RPVGPVTLGSVMSVAELADALHVTAIDVIKALMTQGIMATINQQVDFDTASAVAESFE
                     IEVEEHVPEVLQQASEEIESRRGEGATDPEATPRPPVVTIMGHVDHGKTKLLDAIRET
                     KVAEGEAGGITQHIGAYQVEVQGRKITFLDTPGHEAFTAMRARGAQVTDIAILVVAAD
                     DGVMPQTREAIAHAKAANVPIVVAMNKIDLATANPDRVKQQLSEAGVMPEEYGGDVPV
                     VEISARERMGIDDLLEVLLLVADIGELRANPHKPAVGVVIEAKIDRSRGVTASMLVQS
                     GTLHPRDIIVAGATWGRIRAMTDDRGRKLRKAEPSTPVEILGLFELPQAGDTFQVSED
                     EKTARALAEERTRQRRAEAFSESRVVKLDDIYGQIQEGGTKGLPIILKADVQGSLGAV
                     LGSVAKINDEANQVSLTIVHSGTGAINESDVNLASASGAIIIGFNVRPDAAAKRAADT
                     ANIDIRFYDVIYHLVDDIKLAMSGLLEPEKREVTDGYAEVRDMFRLPNRDVVAGLYVL
                     DGKAARNSRARVLRNGAVMTDTTVGSLKRFKDDAREVQAGYECGAILDNFNDYQTGDQ
                     IEFYHVEEIARTA"
     gene            complement(10686..11024)
                     /locus_tag="VFV93_09380"
     CDS             complement(10686..11024)
                     /locus_tag="VFV93_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX5165593.1"
                     /translation="MSADSATGKPRPKRGARPKHVPQRMCVACREHDAKRTYVRLVRT
                     PEGRVEVDPTGKKNGRGAYLCRRRACWYKAADSNVLERALKVELDAETRAMLREYASS
                     HFPPDETTTT"
     gene            complement(11039..12373)
                     /gene="nusA"
                     /locus_tag="VFV93_09385"
     CDS             complement(11039..12373)
                     /gene="nusA"
                     /locus_tag="VFV93_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX5165594.1"
                     /translation="MKSDFYMAITQIAAERGIPRDAVMSSVEHALKTVYRKTVGASET
                     EDNIDVDIDPATGGVRIFVDRIVAEEVVDPSWQMTLEEARAIDPDAYIGKQYRQDRTP
                     KDFGRIAAQTAKQVVLQRIRDFERDSVYDEFADRVGEVLNGVIQRADSRAVIVELGKA
                     EAVMPVREQVPNERYRPGQRLKVYLVDVNKDPRGPQLIVSRTNVNLVKRLFELEVPEI
                     FSGAVEIMAIAREPGLRSKVAVAARQEKVDPVGSCVGIRGVRIQNIVNELMGEKIDVI
                     EWSSETTTFISNALSPAKPISVGLNDEEKVARVIVPSDQMSLAIGKDGQNARLAYKLT
                     GWRIDVKDPESLKDQDRDLLAEARAALSGVGPEDFMLLGRQPRLVRVDGMFSVRERDF
                     GPLPGDLVGMSVDVEVIDGKVNVFYARDLRARYDFESGQELPLYDDAVAGSE"
     gene            12688..14100
                     /locus_tag="VFV93_09390"
     CDS             12688..14100
                     /locus_tag="VFV93_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX5165595.1"
                     /translation="MKLKTWLRPGMLVKRWILLLLGGLISTSLAMAMGLAYIYRNYEF
                     PSATTGIVQALTLQFIPHPWREIFVLIVGLGVTIFGFLQLSRSLLSPFLDQRTGGQGL
                     ADIIATHRFGSVEPEMRIVTIGGGTGLSTLLRGLKQYNVAITAIVTVGDDGGSTGRLR
                     SEFNVPAPGDIRNCLVALADSETLLSDLFQYRFEAEDSGLNGHSFGNLFITAMTKVAG
                     SFEQAVIESSKVLNIRGAVLPSSLDDISVNAEMDDGTVVSGESNIPRARKRVTRCFIE
                     PSHAEAYDPAIVAILSADLIVLGPGSLYTSVIPNLLVEGIAHALRFAPGERVYVCNVA
                     TQPGETDGYAVIDHLRALADHAGGLPVDRVLINSNLEPAERGIKPEWHVSPVTLDGLH
                     EFEGDIALDQRDIVNPEYPLRHDPQRLSEALIAIGREHRLSLIGSPSGAQGKRATDER
                     RRVQTARGTQIPVETGTGSD"
     gene            14142..15155
                     /gene="gap"
                     /locus_tag="VFV93_09395"
     CDS             14142..15155
                     /gene="gap"
                     /locus_tag="VFV93_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX5165596.1"
                     /translation="MATRIGINGFGRIGRQVFKAIYELHGDDLEIVAANDLGDPHTMA
                     HLLKYDSTYGHFEGDIEVSDGGIVVNGHELKLLAERDPAKLPWGDLGVDLVIESTGFF
                     TDATKAKAHRDAGAKKVIISAPANNEDITIVLGVNDDQYDPDSHHIVSNASCTTNCLA
                     PVAKVLHQHFGISHALMTTVHSYTNDQVILDSPHKDLRRARSAGINIIPTTTGAARAV
                     ALVLPELKGKFDGFALRVPTPTVSIIDLVCEFENDVTVESINAAFQSESEGALEDYLG
                     YSEEPLVSTDYQGTEYSAVVDGLSTMVSGPRMGKVVAWYDNEWGYSCRVADLAALMAA
                     QGI"
     gene            15231..16418
                     /locus_tag="VFV93_09400"
     CDS             15231..16418
                     /locus_tag="VFV93_09400"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5165597.1"
                     /translation="MAARTLDQLDLKGKRVLTRVDFNVPLEDGRITDDTRMTAAIPTI
                     RALLEAGAAVLLVSHLGRPKGKPDPKYSLAPVAAHLRELLGMQVTLAEDVVGPSAQRI
                     SGNLKPGEVAMLENVRFEPGEEKNDPEFARKLADLADVYVNDAFGAAHRAHASTEGVA
                     HLLPSAAGLLMARELDALGKVLSAPEHPVVVLLGGAKISDKIGVINQFMQRADAILVG
                     GGIGNTLLKAEGHQLGTSLVEADALDTASKLLQRAREEGRTLGIPSDVVVAPSVEQAS
                     EARVVAADSIGAEEAALDIGPETVDRYTGVIRTAKTIVWNGPMGLFEVQPFDRGTRAI
                     AEAVAASNAYSVVGGGDSVAALQQSGLADKISHISTGGGASLEFLEGRTLPGVAALEG
                     DAT"
     gene            16415..17170
                     /gene="tpiA"
                     /locus_tag="VFV93_09405"
     CDS             16415..17170
                     /gene="tpiA"
                     /locus_tag="VFV93_09405"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX5165598.1"
                     /translation="MSRGMLVAGNWKMNLDRREAIALAGALAGVAKPSSPVEVGIFPP
                     FPWIVPVRDIVANLHIAVGAQNCHTDVSGAFTGEVSAAMLADVCDYILAGHSERRHIF
                     GESDETVGAKVAAILATSPRPILCVGELLNERNAGRAEEVVARQLDAGLASVAQEDVE
                     RLTIAYEPVWAIGTGVAATPDDAQAMCAFVRSWLIERFGISGGDLRILYGGSVSPDNA
                     ATLFGQADVDGALVGGASLKADSFRAIVAAANA"
BASE COUNT         3012 a         5603 c         5556 g         3112 t
ORIGIN      
        1 ctatccggcc tgatcttcat gctgctgatc atcagccagt tcatcgccta cttcaacttc
       61 agcaacatgc cgaccgtcat cgccggggga atggcccacc tgctcgaaag cgccaacgtt
      121 cacgaattac tgttgctcat cggactgatc gtgatcatcg cggtgctcga catcatcatc
      181 cccggagcga tgccgaagtg ggccatcttc gcgccgatct tcgtcccgct attcatccgc
      241 ctcggtgtcg ccccgcagac ggtactggca gcgtaccgca tcggtgactc gccgatgaac
      301 gtcgttacgc cgctgatggt ttacctgccg ttcatcgtca tcgtctctca gcggtacgtc
      361 aaggagtccg gcattggcac gatcgtgtcg ctgatgatcc cgtacacgct ggtcgttctc
      421 gccatctgga tcatcttctt cgtgatctgg tacgcggtgg gcgtcccgct cggaccgggc
      481 tacggcgtcg gaacctaggg atcacggcca cagtgagagg agcgtgcatc gtgaggggga
      541 cagcgatgaa atctggaatc tggctggtcg gcatcctggc tgcgtccatg ctgctggcgg
      601 gctgcggagg tgaggccagc gccgacagcc tgaccggaac gaagtgggaa ctggacgagc
      661 tcaacggccg tgatgcgctc gacgacgtgc tggtgacgat gcagctcgat gacgacgacg
      721 atctgtccgg aacatccggc tgcaaccgct acatcggcag ttgggaaacg gacgatggcg
      781 acaagatcac gctggaaccc agcggtttga cgatgatggc ctgcgaccag ccggtcatgg
      841 accaggagca ggcgttcctt gaggcgctgg ccagcgcggt ccgcttcgat ctcggcgttg
      901 acgagctgga actctacaac gccgagggcc gtgaggtagc cgagttcgac cggctcaagc
      961 ccatcaacct gatgcggacg ccgtgggtgg tcgtcgcgat caacgacggc gagaaccgcg
     1021 ccgtgcccgt cctcgaaggc acgacgctga tggcgctctt cgagtcggaa gacgaccggt
     1081 tgagcggaca aggcggctgc aataccttca cgacgacgta ctcgcgcgat ggcgaggaga
     1141 tgacgatcga gacgccgatc gcggcaacat tgatggcctg cgaccagcca gtcatggacc
     1201 aggagacgat gttcttcgag gcgctgggac ggaccgcaac cttcgagctc ggcgacgaga
     1261 cgctgctcct gcgctcggaa gacggctcga tgcaggtttg gttcaaggac gcggattagc
     1321 aatcgcctgg gcaacgggat gtatcggagc ttgcgatcga cgatgcacga gtgctgcgtg
     1381 gctggctggg cggctcaagg tcgtcgtggc tggagagaag gacatccgat gttcgctgac
     1441 atactggtgg gactcgcgct cggagcattc ggtctctttg tctgtttcat ggggttgcgt
     1501 ctgttcttct ttgcactccc gatcgtcggg tttgtcaccg gcttctacat cggagtcgcc
     1561 gggacccgcg ccgtgctgca cgagatcttc ctcagcaccg tcacggcagt catcatcggg
     1621 ctcatcgtcg gtgtcgcgct cgggatcctt gcctactttc tgtggtacgt cggcgcgctg
     1681 atctcggctg ggacaaccgg cgcgctgatc gggtcagggc tgatgcatac gttcggcgtg
     1741 acctcgggct gggtcgtgtt cctggtcgcc cttgccgccg gcatcttcct gttcctggtg
     1801 gcattcaagc tcgcgctgcc gatctgggtc gtcatcgtca actcagccat gcttggcgca
     1861 gccggcgccg tcgctggcgt gatgctgatc ttcgatcaaa tcgatcgaag cgacctcaac
     1921 tacgggctgg cctgggccgc gattgaggac tcgtggttct ggattatcgc ctgggcgatc
     1981 gtggcggtga tcgggattgg catgcagtcg caggcgatcg agaagacaac gttgccgcaa
     2041 gaccgctggg gcgccgcgca cgccacctag ctcccggccg cccgttcgcg ctcactcgtt
     2101 acggtgccgc tcggacgact caccagtcgc gcacatgcga ccgtccgaag ttcaccacag
     2161 tcgtgtacat agagagatct ttgatggacg atcaacgacc gaacaacact gatcccggac
     2221 aggaacctga gaacctcgac aacgaggccg tcggcggcac gtctgaacct gctggtgagg
     2281 atatcgcgcc cgatgctcag ccgacgcagc cgcttgcgac tccgccgtca acaccgccag
     2341 gcgcaccgcc gccgagcgac aacgacggac gacaacgctg gatcatcggt ggagtcctgg
     2401 catttgtgct cgtcgccgcc ctgctcgcgc tgttgctctt cagggacgac gacgacggcg
     2461 acgatacgga ggccgaggaa caggccaccg aagaagtcgc caccgaaacg gcgatggcgg
     2521 ccattgaacc gacggcgacg gtagtagaac cgacggcgac gtcggaaccc gaaccaaccg
     2581 agaccgaggt tcaggcgacc gagacgcctg agccagacga cgaagaaacc gaggtcgcgg
     2641 caaccgaaac ggccgaggcc gaacctgagc caaccgaaac caagacggcg ccgaccgaga
     2701 cggccgaggc ggctgaaccg accgcaacca agacaccgga agaagccaag ccgacggaag
     2761 aacccaagcc aacggcaact gaagaggagc ccgaaccgac gccggatgac agcgacctcg
     2821 cctacgaggc tgactggtcg acaggcaaca acggctgggt cctgaccagc ggctgggcga
     2881 tcgagaacgg gaatctcgtc acttccggca atgccgctcc gctgacggct ccgttcgagt
     2941 cacaacgtgc cgattacgcg gtcgagatga cgatgaccgt caccgacctg gatgactgcg
     3001 acgcgcgcgt aggcgtcttc gcccgtgtca ccgacgccgc tggcccgtcc ggcacagtcc
     3061 acgtcggcta ccccgcaagc gtttgcgacg acgcgtggca catcgactcc gtgcttgccg
     3121 acgaccgcga ttcgctcgca agtggcgatc gaccgctcgc aagcggctcg catgactacc
     3181 gcatcgaggt tgccggcgaa cgaattcgtc tgtacatcga cgacgagttc gtcggggcag
     3241 cgaccgataa ccgctggact gaggcgggcc ggggcggaat ctacgtcgac ggagacgctc
     3301 aggtgcagat cagcgcgttc cgcatcttcc tgctctcgga cactccgtga cagggaaagc
     3361 ccataccgag ggcgggttca agctcgaacg actcgtgacc cacgcggccg ccggcgctac
     3421 tgtcgggtca ctctggggcg cgttgattgg tctcgtcttc ctgatgccgg ccgccggcgc
     3481 gatcgtcggt gccgccagcg gggccgtggc cgggacgttc gttggcgtcg ttcgcaccga
     3541 cgacgccaac aaccacggct tcggtcagtt tgggcagcag ttcagcgccg tcatggttct
     3601 cggcagctcc gctgtcctga tgctggtcta cacgaaagac ccggatgggg ccatcgcaac
     3661 gctcaagcag tacagcggtc gcgttctccg ttcgaccctg cccgaccagg tcgaggcgca
     3721 actccacgcc gccctgtcgc agctacccgc aaagcgcctg ctccatccgg accaattgtg
     3781 tcggcgatag atccgacgct tccggtatct tgacttgtag aagggatcaa cagaaatcgg
     3841 agcgggagaa gggactcgaa ccctcgacat tctgcttgga aggcagacgc tctaccaact
     3901 gagctactcc cgcgggttca cggagtcggg gtgagaggac tcgaacctcc gacctcagcg
     3961 tcccaaacgc cgcgcgctac caactgcgcc acaccccggt agaaaccgca ccgatcaagt
     4021 atacatgacg gcttgtgctc agtttcgagg tacgagccgg agaaagcggc gccgtccaac
     4081 ccgcaggatc gccggttcgt tcagcgaaat cagcgttgtc accgagtcga ttacctcgtt
     4141 gttcagccgc acaccgccct gttcaaccaa tcgccgggca tcgttgttgc tggtcgccag
     4201 ccttgttgaa acgaggatcg cctggatcgt cgccggcaga tcgaccaacg tgtcggcgat
     4261 ctcgctcggc gttgcgccgt cgcggaagac gcgttcgaac tctgcctgcg cgaagtcggc
     4321 cgcctcctgg ccgtgtaggt cggcgacgat cgtatgagcc aggatcgcct tcacgtcgcg
     4381 tggattcgtt gcgccggtat gcatcgcctg gacgatggcg tcgatatcgt caccagcgac
     4441 cgatgtgacc aggcggtagt actgctcgat cagtcggtcg ggaatcgaca tcagcttgcc
     4501 gaaggaggtg accgggacct cggcgacgcc aacgtagttg tcggcgctct tggacatctt
     4561 catggccccg tcgagcccga ccagcagcgg caccgtcagc acatcctgtg gcggttgccc
     4621 cgacgcgcgc tggatgtcac ggcccaccag caggttgaag gtctggtcgg tgccgccgat
     4681 ctcgacgtcg gccgctacgg caaccgagtc gtacgcctgt agcagcggat agagcagctc
     4741 tacgatcccg attgacgtgt tctcggctat gcgcttctgg aaatcctcgc gttgcagcat
     4801 ctgggcgacc gagtagcgcg cggttaaccg caggacatcg gccaggtcga acccgccgaa
     4861 ccattcagac tgatgccgga tctcggcccg ctcgacatcg atgacgcgaa agagctggtc
     4921 gaggtaggtc cgcgcgttgt tccgcacctc ttcggcggtc agcatcgtcc gttgggtcga
     4981 tctgccggtc ggatcaccga tctgcgccgt ccagtcgccg atgatgatga cgacggtgtg
     5041 acccagttgt tggaactggc gcaatttcct gaacgcgaca tagtgtccga tatggaggtc
     5101 ggggcgggat ggatcgacgc cgaacttgac gcgtagcgag tcgccggttc ggaggcggcg
     5161 ttccagatgc tcgcggacct gaatcagcgc cacacgtgca tcgctcagca gatccgcgat
     5221 cagtcgatcg cggttggtgt gcatcatcag tccgggttcg caggttcggg ctggttgctg
     5281 gccagaatcg ctcgcgaggt cgcttcgtcg tcctgcatct gcgccgccag tgcttcggcc
     5341 gagtcgaacg cctgatcgcc acgcacccag gcgacaaact cgatttcgag atccagcgtg
     5401 tacaggtcac cgctgaagtc gagaatgttg acctcgacct gccggtcacc gttgtcgaac
     5461 gttggccggg ttccaatgta gatcattgcc gctctggggc cgagcgcacg atccgagaga
     5521 tgtgcgtagc cggcatagat gccgtcggcc gggacgcagg cgtcggcagg tagcaaaagg
     5581 ttcgcggttg gaaagcctag ctcgcgaccg cgcgcggcgc cgtgctcgac cgttcctcgc
     5641 agtcggtagc ggcgcccgag cgaggctgtc gcacgagcga tgttgccgcg cgagagcgcg
     5701 tcacggatgg atgagctgga gatggtgaca tcgccgtcct gcaggcgttc gacgacggta
     5761 gtctcaaagc cgtgtgtttg cccaaatgct tcgatcatgc cgccatcgcc ggctcgcccc
     5821 ttgccgaagc gaaagccgga gccaacgaag acggccaccg gctggaccgt cctggcgagc
     5881 aactccagga actcctcagg ctcgagggcg gcgaactcgc gatcgaacgg gatgacggcg
     5941 atgtcgtcaa cgccggcggc gttgaggatc tcgagctttc gctcaggtga cgcgaggcgg
     6001 tggaacggca cgtcggggcg cagcaccgcc gtcggatgcg gctcgaacgt caccaccagc
     6061 gatcggcttc cgcgggcaac ggcggcgtcg atcacgcgct ggatcagata ttggtgacca
     6121 cgatggacac cgtcgaagtt gccgatggtc atgacatgcg gaacgcgagc gatgcgttcc
     6181 agcgtcgcga tagcaccctc agggacctgc gtcacgattc ttcaccctca tcttctggcg
     6241 gcaacacgag cgatggcttc aaggcattgt ccgcggtccg ggttccaatt ccgaggaagc
     6301 gtccgtcgtc accgtaggcg cgcactggtg cattggcgga ctggctggca ggtgaaagct
     6361 ggaccgatct gccatgatac catgcggcct ggtcgccctc gccgaggacg acggccggca
     6421 ggtccgcaag agctgcgtcc ggatgtaatg cgatctgcgg ccactcctga cggacgtcgc
     6481 gttcggtcag ctcatccagc gtccaagcgt cagcgaggca gaatcggccg gtgttggtcc
     6541 gacgtagccc gtggcaatag gcctcagtcc cgagcgcgac gccgatgtcg cgcgcgattg
     6601 aacggacgta cgttccctta ccgcagtgga tatcgaggat caggtcgggc ggccggtagg
     6661 agacggtgtc gatggtgtgg atcgtcacca tacgtgtcgg cacttcgacg tcctcgccgg
     6721 cccgcgccag ctcgtagagc tttcggcctc cgaccttgat ggcggagtag gcgggcggca
     6781 cctgctcgat ctcgccgacg aagcgcgcga ccgtcgtcgt gacctcttcg tggcccggcc
     6841 agccgccgcc atcgccgatc tcgttcagga cgccgtcgac gtcagcggtg tcggtcgagg
     6901 cgccgaggac gacgtgtgcg cgataaccct tctggctgtc ctggacgtat tgcagcaggc
     6961 gggtggcgcg gccgacggcg acgacgagga tgccggtcgc gaacgggtcg agtgtgccac
     7021 cgtgaccgac gcgcttcatg ccggtcagcc ggcggacgcg gccgacgaca tcgtgcgagg
     7081 tcatcccggc cggcttgtcg atcaccagca agccgttcag atcgggcaat ttagaccggg
     7141 tcatcaccac cactcgcgcg tcctgtcagg gcgccgcgcg gaccgagcag accggcgacc
     7201 tcattcagga atcgctcctt ggcgtcctcg ccggcgtcca gccgggctcc ggccgcgcgc
     7261 gggtgtccgc cgccgccgaa gatgcccagc agcttggcga cgtcgacgtc gtcggccagc
     7321 gaacgcatgc tgacccgcca gccccacgac tcccggtaga gcagtgctga cgcccgcgcg
     7381 ccgatcgtgc cggccaggaa gttgacgatg ccctccgctt ccgtccggtc ggctcccgag
     7441 cgctccagca tttcagggtc aatctgggtc cacatcagcg cgccgcgcca ctgagcgcgc
     7501 tgaagggcct cggcccagag gcagatcgtc gaatacggct tgagccggaa gagcgcgtca
     7561 acgatcgaat ccatgtccgc gccggcttcg atcagttcgg cggccgtccg cagcgatgct
     7621 ggcgtcgtgc tgggagtgcg caggccgagc gtgtctccgt agatgccagc cagcagcgtc
     7681 gtcgccgtgt ctgcgtcgat ctcgacgccc agctcatcga agaggccgac gacgatttcg
     7741 gctgtgcttg cggcggccgg gacgacgaga ttcagcgtcc cgaagcggtg gttggtaacg
     7801 tggtggtcga cgttgatgat cggtcgcgtg cgttcgaact cggtcgccat ccggtagaag
     7861 agcggaccga gccgggacgg gtccgagcag tcaaccagga tcagcagatc agcctcggca
     7921 actgcctcat cggcagcatg cttcagatcg agcgcgccga ccaggaattc gaggttcgac
     7981 ggcagggccg cgtcaccgga cgaagtcagc accggctcga cgccgaaact ttgcaccagc
     8041 gtccgcatgc ccagcgcggc tgcgacagcg tcggcgtccg ggttggcatg tgtcaggatg
     8101 accggccggg acgacccacg gaccgcgtca cggatcgcgt gagcggcggc cgggatactg
     8161 cgcagcgtct cgtgaccgtt cgcggccggt tcgctgccgt tctcctggaa cgagtcgatc
     8221 acgcgggtgt cccttcatct gtcgatgccg gctcgttctc ttttgcttcc gccgcacgca
     8281 gttctggttc gaggtcgcgc aggatgcggg cgacgttctc ggcgtgctcc atcgaccggt
     8341 cgagccggaa cgatacttca ggaatcaccc gcatgcgaag gcgtggcgca agctggcggc
     8401 gaatgaagcc gctggcgttg ttgagcgcga ccagcgacga cgcgcgctca tcctcgctgc
     8461 ccatcacact gacgaacatc cgcgcgtact tcagatcggg gctgagttca acacgagtga
     8521 tgctgacgaa accgagccgg gggtcgctca tctcacgctg gatgatttcg ctgacctcgt
     8581 cacgcagcag atcaccgacc cgctcgcgcc gccgtccgct catggactcc ccctctgctg
     8641 cgtcccgata cgctgaggcg acagtcggtt gactgccgcc cgttcgatgc gaagttcacc
     8701 ggcacgccta ggcggtccgg gcgatctcct cgacatgata gaactcgatc tgatcaccgg
     8761 tttggtaatc gttgaaattg tccagaatcg cgccgcactc gtagccggcc tggacctcgc
     8821 gcgcgtcatc cttgaagcgc ttgagtgagc cgactgtcgt gtcggtcatt acggcgccgt
     8881 tgcgcagcac gcgtgcccgc gagttgcggg ccgccttgcc gtcgaggacg tacagtccgg
     8941 cgacgacatc gcggttcggc agccggaaca tgtcgcggac ctcggcgtag ccgtcggtca
     9001 cttcgcgctt ctccggctcc agcaggcccg acatcgccag cttgatgtcg tcaaccaggt
     9061 ggtagatgac atcgtagaag cgaatgtcga tgttggccgt gtcggcggcg cgcttggcag
     9121 ccgcgtccgg ccggacgttg aagccgatga tgatcgcgcc cgacgctgag gcgaggttga
     9181 catccgactc gttgatcgcg cccgtcccgg agtggacgat cgtcagcgag acctgattcg
     9241 cctcgtcgtt gatcttggcg accgagccaa gcacagcgcc gagcgatccc tgaacgtcgg
     9301 ccttgaggat gatcggcagt cccttggtgc caccctcctg gatctggccg tagatgtcgt
     9361 ccagcttgac aacgcgtgac tcggagaacg cctcggcgcg tcgctggcgg gtgcgctcct
     9421 cggccaatgc gcgtgccgtc ttctcgtctt ccgacacctg gaaggtgtca ccggcctggg
     9481 gcagctcgaa cagaccgaga atttcgacgg gtgttgacgg ctctgccttg cgcaacttgc
     9541 gaccgcgatc gtcggtcatc gcgcgaatcc ggccccaggt ggcgccagcg acgatgatgt
     9601 cgcgcggatg cagcgtgccg gactggacca gcattgacgc cgtcacacca cggctgcgat
     9661 cgatcttggc ctcgatgacg acaccaacag ccggcttgtg cgggttggcg cgtagctcgc
     9721 cgatatcggc caccagcagc agaacttcga gcagatcgtc gatacccatc ctctcgcgag
     9781 ctgagatctc aacgaccggg acgtcgccgc cgtactcttc cggcatgaca ccggcttcgg
     9841 agagctgctg cttgacgcgg tcagggttgg ccgtcgccag gtcgatcttg ttcatcgcga
     9901 cgacgatcgg cacgtttgca gccttcgcgt gggcaatcgc ctcgcgtgtc tgcggcatca
     9961 cgccgtcgtc ggccgcgacc accaggatcg cgatatcggt cacctgcgca ccacgggcac
    10021 gcatggccgt gaacgcctcg tggcccggcg tgtcgaggaa cgtgatcttg cggccctgga
    10081 cctcgacctg ataggcgcca atatgctgcg tgataccgcc ggcctcgccc tcggcgacct
    10141 ttgtctcgcg gatcgcgtcg agcagcttag tcttgccgtg gtcgacgtgg cccatgatcg
    10201 tgacgaccgg tggtcgcggc gtcgcttcag ggtcagtcgc gccctcgccg cgccggctct
    10261 cgatctcttc ggacgcctgc tggagcactt ccggtacatg ctcctcgacc tcgatctcga
    10321 aactctcggc aacggctgag gccgtgtcga agtcaacctg ctggttgatc gtcgccatga
    10381 tgccctgcgt catcagtgcc ttgataacgt cgatcgcggt cacatgcagc gcgtctgcca
    10441 actcggcgac cgacatcacc gaaccaagcg tgaccggccc gaccggtcgc tggatcgtcg
    10501 cggtattgcc gccgcgatta cggggtccgt ggtcgccgcg cggtcctccg cgtcggccac
    10561 ctccggggcg ccggttcctg ttcgatccgc caccacccgg cctgcgaaac tgtccactca
    10621 taaaatgctc tcctccatac cacccgtggt cccgatgtgt gggacgttat tgccaggacg
    10681 ggaactcacg tcgttgtcgt ctcgtcaggc gggaaatggg agctcgcgta ctcccgcagc
    10741 atcgcgcgcg tctcagcatc cagctcgacc ttcagcgccc gttcgagcac attcgagtcg
    10801 gccgccttgt accagcacgc gcggcggcga cagagatagg cgccgcgccc gttcttctta
    10861 ccggtcgggt caacttctac ccggccctcc ggcgtccgga ccagccggac gtaggtccgc
    10921 ttcgcgtcgt gttcgcggca cgccacacac atccgctgtg ggacgtgctt tggccgcgct
    10981 ccacgctttg gcctcggctt gccggttgca gagtcggcgc tcatcgtcgc gccctcggtc
    11041 attcgctgcc cgcaacggcg tcatcgtaga gcggcaattc ctgaccgctc tcgaagtcgt
    11101 agcgcgcgcg caggtcgcgg gcgtagaaga cgttgacctt gccgtcgatc acctcaacgt
    11161 cgacgctcat gccaaccagg tcgcccggca gcgggccaaa gtcccgctcg cgaaccgaga
    11221 acatgccgtc cacccgcacc agccgtggct ggcggccgag cagcatgaag tcttccggtc
    11281 cgacgccgga gagcgccgct cgcgcctcgg ccagcaggtc gcgatcctga tccttcagcg
    11341 attccggatc cttgacatcg atccgccagc cggtcagctt gtaggcgagc cgggcgttct
    11401 gcccgtcctt gccgatcgcc agcgacatct gatcgctcgg gacgatcacc cgcgcgacct
    11461 tctcctcgtc gttcaggccg acgctgatcg gcttggccgg cgagagggcg ttcgagatga
    11521 acgtcgtcgt ctctgacgac cactcgatca cgtcgatctt ctcgcccatc agctcgttga
    11581 caatgttctg gatgcggacg ccacgaatac cgacgcacga gccaactggg tcgaccttct
    11641 cctgacgggc cgcgacggct accttgctgc gcaggccagg ctcgcgggca atcgccatga
    11701 tctcgaccgc gcccgagaag atttccggca cttcgagctc gaacaaccgc ttgaccaggt
    11761 tcacgttcgt ccggctgacg atcagctgcg ggccgcgtgg gtccttgttg acgtcgacca
    11821 gatagacctt taagcgctgt cccggtcggt agcgctcgtt gggcacctgc tcgcgaaccg
    11881 gcatgaccgc ttcggccttg cccagctcga cgatgacggc ccgcgaatcg gcacgctgga
    11941 taacgccgtt gagcacctcg ccgacccggt cggcgaactc gtcgtagacg ctgtcgcgct
    12001 caaagtcgcg aatgcgctgg aggacgacct gcttggccgt ctgggccgcg atccggccga
    12061 agtccttcgg cgtccggtcc tgcctgtact gcttaccgat gtaggcgtcc ggatcgatgg
    12121 cgcgggcctc ttcgagcgtc atctgccaac tcgggtcgac gacctcctcg gcgacgatcc
    12181 ggtcgacgaa gatacggaca ccgccggttg ctggatcgat atccacgtcg atgttgtcct
    12241 cggtttcgga cgcgccgacc gtcttgcgat agacggtctt caacgcatgc tcgaccgacg
    12301 acatgactgc atcgcgcgga atcccgcgct cggcggcaat ctgggtgatt gccatgtaga
    12361 aatcactctt cattgctggt gtatacctcc ttggaactca ggacgtggag gtgtccgctg
    12421 acgaatgacg gcgcatcggg gacgctggcc aatcggccgc gaggctcgtg acgcccgtca
    12481 gcatggagac ggtgcccgga caacaaacga agtgggcgtt gcccacttcg gaatctcggg
    12541 cataatcagt tccacttcga aagtatacca gagtgtggca ggcttcaagt gcgccacaag
    12601 tggtgccggc gcgcggattc gcgggcgtga gtgcatgcta tactggttcc gccagaaacg
    12661 ccgagcggag aggcagtaat cacgagcatg aagctcaaaa cgtggctccg tcccgggatg
    12721 ctggtcaagc ggtggattct cctcctgctc ggcggtctca tctccaccag cctcgcgatg
    12781 gcgatgggcc ttgcctacat ctatcgcaac tatgagttcc ccagcgcgac gaccggcatc
    12841 gtccaggcgc tcaccctgca gttcatcccg cacccctggc gcgagatctt cgtcttgatc
    12901 gtcggtctcg gcgtcacaat cttcggcttc ctgcaactct cgcgctcgtt gctgagtccg
    12961 ttccttgacc agcgcacggg cggccagggg ttggccgaca tcatcgcgac ccaccggttc
    13021 ggttccgtcg aaccggagat gcgcatcgtc acgatcggtg gtggaaccgg tctgtcgacg
    13081 ctgttgcgtg gcctcaagca gtacaacgtc gccattactg caattgtcac tgtcggtgac
    13141 gacggcggct cgaccggccg gctgcgctcc gagttcaacg tcccggcgcc gggtgacatt
    13201 cgcaactgcc tcgtcgcgct ggcggattcg gagacgttgc tgagcgacct gttccagtac
    13261 cgcttcgagg cggaggattc cggtctgaac ggccactcgt tcggcaacct gttcatcacg
    13321 gcgatgacga aggtggccgg cagcttcgag caggccgtca tcgaatcatc gaaggtcctg
    13381 aacattcgtg gagcggttct gccatcgtcg ctggacgaca tcagtgtcaa cgccgagatg
    13441 gatgacggaa cggtcgttag cggcgagtca aacatccctc gcgcccgcaa gcgggtgaca
    13501 cgctgcttca tcgagccgag ccacgccgag gcatacgatc cggccatcgt cgcgatcctc
    13561 tcggccgacc tgatcgtgct cggccccggc agcctctaca catcggtcat ccccaatctg
    13621 ctcgttgagg gaatcgcgca tgcgttgcgc ttcgcccccg gcgaacgcgt ctacgtctgc
    13681 aacgtcgcga cccagccagg cgagaccgac ggctacgcgg tcatcgacca cctgcgggcg
    13741 ctggccgatc atgctggcgg gttgccggtc gatcgggtgc tgatcaattc caacctggag
    13801 ccggccgagc ggggcatcaa gccggagtgg cacgtcagcc ccgtgacact cgacgggctc
    13861 cacgagttcg aaggcgacat cgcgctcgac cagcgcgaca tcgtcaaccc tgagtacccg
    13921 ctgcgtcatg acccgcagcg tctctccgag gcgctgatcg cgatcgggcg ggagcaccgg
    13981 ctgagcctga tcggctcgcc gagtggcgct caggggaaac gtgctaccga tgagcgtcgc
    14041 cgcgtccaga cggcacgcgg cacgcaaatt ccggtggaaa ccgggaccgg ctccgactag
    14101 ggacgggccg gccagacgta tggcgcagga ggagggacca cgtggcgacg cgcataggca
    14161 tcaacggctt cggccggatc gggcggcagg tcttcaaggc gatctacgag ttgcatggtg
    14221 acgatctgga gatcgtggcg gccaacgacc tcggtgatcc acacacgatg gcgcatctgc
    14281 tgaagtacga ctcgacctac ggccacttcg aaggcgatat cgaagtctcc gacggcggga
    14341 tcgtcgtcaa cggccacgag ctcaagctgc tggccgagcg tgacccggcc aagctgccat
    14401 ggggcgacct cggcgtcgat ctcgtcatcg aatcgaccgg cttctttacc gacgcgacca
    14461 aggcgaaggc gcaccgcgac gccggcgcga agaaggtcat catctcggcc ccggccaaca
    14521 atgaggacat cacgatcgtg ctcggcgtca acgacgacca gtacgatccc gactcccacc
    14581 acatcgtttc gaacgcgtcg tgcacgacca actgcctcgc gccggtcgcg aaggtgctgc
    14641 accagcactt cggtatcagc cacgcgttga tgacgaccgt ccactcgtac accaacgacc
    14701 aggttatcct cgacagcccg cacaaggatc tgcgccgcgc acgcagcgcc gggatcaaca
    14761 ttatcccgac gacgaccggc gccgcccggg ccgtcgcgct ggtcctgccg gagctgaagg
    14821 gcaagttcga cggcttcgcg ctgcgcgtgc caacgccgac cgtctcgatc atcgacctcg
    14881 tctgcgagtt tgagaacgac gtgacggtcg agtcgatcaa cgccgcgttc cagagcgagt
    14941 ccgagggcgc gctcgaggac tacctcggct actccgagga gccgctcgtc tcgaccgact
    15001 atcaggggac cgagtactcg gccgtcgtcg atggcctctc gacgatggtc agtggtccac
    15061 ggatgggcaa ggtcgtcgcg tggtatgaca acgagtgggg ctattcctgc cgcgttgccg
    15121 acctggcggc gttgatggcc gcccagggga tttaaccgac tccagtctga accaggaggc
    15181 gggcggcagc gatgccgccc gtcaacgcat catcagaagt ggaggaagtg gtggcagctc
    15241 gcacgctcga ccaactcgac ctgaagggca agcgcgttct gacacgggtt gacttcaatg
    15301 ttccgcttga agacggccgg atcaccgacg acacccggat gacagccgcg atcccaacaa
    15361 ttcgcgcctt gctggaagcc ggcgcggcgg tcctgctcgt ctcacacctc gggcgtccaa
    15421 aagggaagcc cgatccgaaa tactcgctcg cgccggtcgc cgcccacctc cgcgagctgc
    15481 tggggatgca ggtgacgctc gctgaggatg tcgtcggacc gtcagctcag cggatctccg
    15541 gcaatctgaa gccgggcgag gtggcaatgc tcgaaaatgt tcggttcgag ccgggcgaag
    15601 agaagaacga ccccgagttc gcccgcaagc tggctgatct ggccgacgtg tacgtcaacg
    15661 acgcgttcgg cgccgcccac cgcgcgcacg ccagcaccga aggtgtcgcc catctgctgc
    15721 caagcgcggc cggcctattg atggcgcgtg agcttgatgc gcttggcaag gtgctgtccg
    15781 caccggagca tccagttgtc gtcctgctgg gaggcgccaa gatctctgac aagatcggcg
    15841 tcatcaacca gttcatgcag cgggccgacg cgatcctcgt cggcggtggt atcggcaata
    15901 cactgctgaa ggcggagggc catcagctcg gcacgtcgct ggtcgaggcc gacgcactcg
    15961 acacagccag caagctgctc cagcgggcgc gtgaggaggg tcgcacactc ggcattccat
    16021 ctgacgtcgt cgtcgcaccc tcggtcgagc aagcctccga ggcgcgcgtt gtcgcggcag
    16081 acagcatcgg tgcagaggag gcggccctcg acatcggtcc cgagacggtc gatcgctaca
    16141 caggcgtcat ccggaccgcg aagacgatcg tctggaacgg cccgatgggc ctcttcgagg
    16201 ttcagccgtt tgatcgcggg acgcgggcga ttgccgaagc ggtcgctgcc tcaaacgcgt
    16261 actcggtggt cggcggcggt gattcggtgg ccgcgttgca gcagtcgggg ctggccgaca
    16321 agatcagcca catctcgacg ggcggtggtg cgtcgctgga gtttcttgag ggacgaacgc
    16381 tgccgggcgt ggccgcgctg gaaggagacg cgacatgagc cggggaatgc tggtcgccgg
    16441 taactggaag atgaacctgg accggcgcga agcgatcgcg ctggccggtg cgctggcagg
    16501 agtagccaag ccgtcctcgc cggtcgaggt cggcatcttc ccaccgttcc cctggatcgt
    16561 cccggttcgc gacatcgtcg ccaacttgca catcgccgtt ggcgcccaga attgccacac
    16621 cgacgtctcg ggcgcgttca ccggcgaagt ctcggccgcg atgctggcgg acgtctgcga
    16681 ctacatcctg gccggacaca gcgagcgtcg ccatatcttc ggcgagtccg acgagacggt
    16741 cggtgcgaag gttgcggcga tcctggcgac gagtccgcgt ccgatcctct gcgtcggcga
    16801 gctgctgaat gagcgcaacg cgggccgggc cgaggaggtt gtcgcccgcc agctcgacgc
    16861 cggactggcc agtgtcgcgc aagaagatgt cgagcggctg acgatcgcct acgagccggt
    16921 ctgggcgatc gggacgggtg tcgcggcgac gccggacgac gcccaggcga tgtgcgcctt
    16981 cgtccgaagc tggctcatcg aacgcttcgg aatcagcggg ggagatctgc gaatcctcta
    17041 cggcggcagc gtctcgcccg acaacgccgc aacgctgttc ggccaggctg acgtcgacgg
    17101 cgcgctggtc ggcggagcca gcctgaaggc cgattcgttc cgcgcgattg tggccgccgc
    17161 caacgcctga atgtagtatc cttcgcccga aacggattcg ttcagacccg gaggcttcgc
    17221 gttggatgtc gcgctcgacg tcgtacagat tatcgtaagt gccttgctga tcctcgtcat
    17281 cct
//
LOCUS       DASUGJ010000425         3515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1453937, whole genome shotgun sequence.
ACCESSION   DASUGJ010000425 DASUGJ010000000
VERSION     DASUGJ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453937"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFV93_09410"
     CDS             <1..418
                     /locus_tag="VFV93_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165599.1"
                     /translation="WRVVDLRRDNYPYYLSSDDVRALESLDDVAGRRDIVMSSPNLGL
                     FVPVYSDARPFVAHWAQTLRYFERRDQARWFFSARTSDAEREVFLRDNGIDYIISGPA
                     EAADSAVSAPPRLPLDAIGGESTVLYRVRISSGSSR"
     gene            561..2276
                     /locus_tag="VFV93_09415"
     CDS             561..2276
                     /locus_tag="VFV93_09415"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX5165600.1"
                     /translation="MRTPVMTNVYVEFIRRWYWIIIIGVIVGVVSTNMALSRQIPVFR
                     STATVQVGRTIQDSKPELVNLSITDQLVPVYQELARRDRVLNAVSSSLGLPYDADDLR
                     ARLMVQQVSGTQLIDIKVVDADPELAAAIANEIARQLVLQGPDETDTDDSQQFVQAQL
                     ADLQAKITSGQIQIETFEEQVSGSTSAADVFDAQARLDALNAQVNTWQASYAALIANA
                     EPSKTNFLEVVGTAVPATMPLPRPAKLYYGLGILVGGALAAAIAFALSALFGAIRRAE
                     ELRKLSNNVPITTIPYLREIRKQPLVSSSAPASPTAASYRILRNVLQTEQSDAAPVSI
                     AVFSSRPGEGKTTTVANLAIALASVRRNVILVDANVRNPSLDHMFGTDTSRGLSDVVL
                     GECELADALQPTAYPNLTILGAGSIPGNYTDILSPGRVRKVVEAVTAMAEITLIDTPS
                     IQEEQEALMLAKEVDAAIVIVEAGRIRAGEAQRTLAALSRSGVPVLAIALSKARTPRG
                     IFERLPFSRDARLRKLAESRRSARQHPEPQQRGQRPRLTPLNQSKRSVTHAQSITHTD
                     RSKSA"
     gene            2233..3495
                     /locus_tag="VFV93_09420"
     CDS             2233..3495
                     /locus_tag="VFV93_09420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5165601.1"
                     /translation="MRSLLLTQIVPNPPDAGPKAKTHYVLRTLAAEHDVDLITFARSD
                     LEVAQAEDLAPFCRSITAIRLERQRPMELVYFAKSWARRTPFLVERDARGAFAEAVRR
                     QLATGAVDIVHADQLTMGQYLDLARGHHTRRVFDAHNAVWEMVRELAPRQKTPVHRAL
                     AAVEWRRLRRFEGRIARESDLTLAVSERDRAALELAAGTTINAAIVPIGIEVHDRTVV
                     TPSRSTNLLSIATMHYPPNAEALRWFLDEVWPLLGADRSEMTVDVAGPRPPEDIVAAG
                     ERDPRVRVHGYVEDVEPLYQQAAVFIVPLLSGSGVRVKILEAMARGVPVVSTTIGADG
                     LDLDDGVHLLIADTPAAFADAVTYLLEHPERAQAMAVSGRERILERYDWRSCCRPLLD
                     AYRQLSGDPAGETAGSMGFSIASARDVT"
BASE COUNT          603 a         1126 c         1127 g          659 t
ORIGIN      
        1 ctggcgcgtg gtcgatcttc gacgagacaa ttatccctac tacctgtcca gcgacgatgt
       61 ccgggcgctc gaatcgctcg acgacgtcgc ggggcggcgt gacatcgtca tgtcatcgcc
      121 caacctcggc ctgttcgtgc cggtctactc ggatgcacgg ccgttcgtcg cccattgggc
      181 ccagacgcta cgctacttcg agcgtcgcga ccaggcgcgc tggttcttct cggccagaac
      241 gtcggacgcc gagcgcgagg tcttcctacg cgacaacggc atcgactaca tcatctccgg
      301 cccggctgag gcggccgata gcgccgtgtc tgcgccaccc cgcctgccgc tcgacgcgat
      361 cggcggcgaa tcgacggtcc tgtatcgggt tcggatctca tctgggagca gccgataaac
      421 gatctggccg gctcgcttgt cctgtgcccc ctgctgctga cctagactga cattctcgtc
      481 accaatttgc caacttcttg caagcaccgc tgcgggtgcc cgaggtgaca cttgatgcgc
      541 ggtgtcgata tgcaggaagg ttgcgcacgc cggtgatgac caacgtctac gtcgagttca
      601 tccgtcgctg gtactggatc atcattattg gtgttatcgt cggtgtcgtt tccacgaaca
      661 tggcgctgtc acgccaaatc ccagtttttc ggtctactgc gacagtgcag gtcggtcgaa
      721 cgattcagga cagtaagccc gagttggtga atctgtcgat cacggaccaa ctcgttccgg
      781 tctatcagga gttggcgagg cgtgaccgtg tcctgaatgc tgtctccagt tcgctcggct
      841 tgccgtacga cgccgatgac ctccgggcgc ggttgatggt ccagcaggtc tcggggactc
      901 aactgatcga tatcaaggtc gttgatgccg atccggaact ggcggcggct atcgccaacg
      961 agatcgcccg ccagctcgtg ttgcagggcc cggacgaaac agacaccgac gactcacagc
     1021 agtttgttca ggcgcagctg gccgatctgc aggcaaagat caccagcgga cagatccaga
     1081 tcgagacctt cgaagagcag gtctcgggat cgaccagtgc cgccgatgtc ttcgacgccc
     1141 aggcccgtct ggatgcgctc aacgcccagg tgaacacctg gcaggcatcg tacgcggcgc
     1201 tgatcgcgaa tgcggaacca agcaagacga acttcctgga agtcgtcgga acggcggtcc
     1261 cggccacgat gccgctgccc aggcccgcca agctctacta tgggctgggt atcttggtcg
     1321 gtggcgcgtt ggcggcggcg atcgcattcg cgttgtcggc gctgttcggc gccatccgcc
     1381 gcgcggaaga actgcgcaag ctgtcgaaca acgtcccgat aacgacgatc ccctacctgc
     1441 gtgagatccg gaagcaaccg ctggtctcgt cgtccgcgcc ggcctcgccg acggctgcgt
     1501 cctaccgtat cttgcgaaat gtgctccaga ccgagcagtc ggacgccgcg ccagtctcaa
     1561 tcgccgtctt cagcagccgg cccggtgagg ggaagacgac gacggtcgcc aaccttgcca
     1621 tcgccctcgc cagtgttcgg cgcaacgtca tcctggttga tgccaacgtc cgtaatccca
     1681 gtcttgacca catgttcggg accgatacgt cgcgtggtct cagcgacgtt gtcctcggcg
     1741 agtgcgagct ggccgatgca ctacagccga ccgcctaccc gaacctgacg attctcggag
     1801 cgggtagtat ccccggcaat tacaccgaca ttctctcgcc ggggagggtc cgcaaggttg
     1861 ttgaggcggt gacggcgatg gctgagatca cgctgatcga cacgccgagc atccaggagg
     1921 aacaggaagc tctgatgctg gcgaaagagg tagacgcggc catcgttatc gtcgaggcgg
     1981 gtcgtatccg tgctggcgag gcccagcgga cgttggcggc cctctccaga tcgggtgttc
     2041 ccgtactggc gatcgcgctc agcaaggcac ggacgccgcg cggtatcttc gagcgcttgc
     2101 cgttctcacg cgatgcacgg ctgcggaagt tggccgaatc gcgccgctct gcccggcagc
     2161 atccggaacc gcagcagcgc ggacagcgcc cgcggttgac accattgaat cagtcgaaac
     2221 gatcggtgac gcatgcgcag tctattactc acacagatcg ttccaaatcc gcctgacgca
     2281 gggccgaaag ccaagacgca ctacgtcctg cggacgctgg cggctgagca cgacgttgac
     2341 ctgatcacat tcgcccgctc ggacctcgaa gtcgctcagg ccgaagatct tgcgccgttt
     2401 tgccggtcga tcaccgccat ccggctcgaa cggcagcgcc cgatggagct ggtctacttc
     2461 gcgaagagct gggccagacg gacaccgttc ctggtcgagc gcgatgcgcg cggggcattc
     2521 gcggaggcgg ttcgccgtca actcgcgacc ggtgctgtcg atatcgtcca tgccgaccag
     2581 ctcacgatgg ggcagtatct ggatctggcg cgcggccatc atacccggcg tgtcttcgac
     2641 gctcacaacg ccgtctggga gatggtacgc gagcttgcac cacgccagaa gacgcccgtt
     2701 caccgggcgc tggcggccgt cgagtggcgg cggctgcggc ggttcgaggg ccggatagcg
     2761 cgcgagagcg acttgacgct ggccgtttcc gagcgggatc gggctgcgct ggaactggcg
     2821 gccggtacca ctatcaacgc cgcgatcgtg ccgatcggga tcgaggtcca cgaccgaacg
     2881 gtggtgacgc cgtcccggtc gaccaacctc cttagtatcg cgacgatgca ctacccaccc
     2941 aacgccgaag cgttgcgctg gttcctcgac gaagtctggc cgctgcttgg cgctgaccgt
     3001 tccgagatga cggttgacgt ggccgggccg cgtccgccag aggacatcgt cgctgccggc
     3061 gagcgtgatc cgcgggtccg ggtccatggc tatgtcgagg acgttgagcc gctctaccag
     3121 caggcggcgg tcttcatcgt cccgttgctg tcggggagcg gagtgcgggt caagatcctg
     3181 gaggcgatgg cgcggggagt gccggtcgtc tcgacgacga tcggtgccga tggactcgat
     3241 ctcgacgatg gtgtccacct gctgatcgcc gacacgccgg cggcctttgc cgatgccgtg
     3301 acgtacctgc tggagcaccc cgagcgggcg caggcgatgg ccgtttccgg acgcgagcgc
     3361 atcctcgaac gttacgactg gcgcagctgc tgccggccgc tgctcgacgc ctaccggcag
     3421 ttgagcggcg atcccgctgg tgaaactgcc ggatcgatgg gcttttcgat cgcgtctgcc
     3481 cgcgatgtga cgtgattgtc accctcgatt gacgg
//
LOCUS       DASUGJ010000426         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_483911, whole genome shotgun sequence.
ACCESSION   DASUGJ010000426 DASUGJ010000000
VERSION     DASUGJ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483911"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VFV93_09425"
     CDS             complement(<1..669)
                     /locus_tag="VFV93_09425"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX5165602.1"
                     /translation="MLRTPLCDLLGIDVPIVQAPMIQAATARLAAEVSNAGGLGSLST
                     VMLPAAKLREEIAQVQERAERPFNVNHIMNQFDPEAWQATLEAWPPVISFALGDPGEY
                     VEQAHAVGSLVMQQVVTVASARRAAERGVDVIIAQGSEAGGNSGLVSTLALVPQVVDA
                     VAPIPVVAAGGIADGRGLAAVLMLGAQGVNMGTRFLASREATIPDGWKQAILDADSSG
                     VGKFV"
     gene            858..1175
                     /locus_tag="VFV93_09430"
     CDS             858..1175
                     /locus_tag="VFV93_09430"
                     /inference="COORDINATES: protein motif:HMM:NF020382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HEX5165603.1"
                     /translation="MQIQIINFQLRELSVEEFHAVCDSIAPQWAAIPGLISKVWLENE
                     QTNTYGGVYTWESREALESYLQSDLFNGIMTNPHFANATTTDFDIIEGPTRVTHGLVA
                     AAV"
     gene            1237..1611
                     /locus_tag="VFV93_09435"
     CDS             1237..1611
                     /locus_tag="VFV93_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165604.1"
                     /translation="MAGYPDAFGISVTVDDLNKARRFYTSLYPHDHVSEGVFAGINYI
                     GIMRDGETLVNVFQAGKNNPLDDMIPILKVDSVVSYQETVRALGGEVVIPTSSCPCTQ
                     TSFAVCVDSTGNQFMIKEPRSS"
     gene            1672..>2403
                     /locus_tag="VFV93_09440"
     CDS             1672..>2403
                     /locus_tag="VFV93_09440"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5165605.1"
                     /translation="MARRLGDVLAQRASETFVGRTDELAELLTILDDDRPIVTCLHGI
                     GGIGKSSLLDAFKLRARDAGATVISLDGRVIEPTPRGFLQELGMAIGLDATTPALAVD
                     RLESLGQRVVLTIDTYELLRLIDTWLRQDFLPLLPENVRIVISGREPPVSAWLSSPGW
                     QDMFHSIRLGSLSEEDAITLLQQRGIDDVSALRINRFADGHPLALQLAAANVHEPSRL
                     DFADVAVQRVVEELARTYLADVPDPL"
BASE COUNT          466 a          816 c          678 g          443 t
ORIGIN      
        1 cacgaacttg ccgaccccgc tcgaatcagc atccaggatc gcctgcttcc agccgtccgg
       61 aatcgtcgcc tcacgcgagg ccaggaagcg cgtccccatg ttgacgccct gcgcgccgag
      121 catcaggacc gccgccaacc cgcgcccatc agcgatcccg ccggccgcga cgaccgggat
      181 cggcgcgacg gcgtcgacaa cctgtgggac gagcgccagc gtactgacca gccccgagtt
      241 gccgcccgcc tcgctcccct gggcgatgat gacatcgacg ccgcgctcgg ccgcccgccg
      301 cgccgaagcg accgtcacga cctgctgcat gacgaggctg ccaaccgcgt gcgcctgctc
      361 gacgtactcg cccggatcac cgagcgcgaa cgagatgacc ggcggccagg cctccagcgt
      421 cgcctgccat gcctccgggt cgaactggtt catgatgtgg ttgacgttga acggccgctc
      481 ggcccgctcc tgcacctgcg cgatctcctc acgcagcttc gccgccggca gcatgacggt
      541 cgacaagctc cccagcccgc cggcattcga cacctcggcc gccagccgcg ccgtcgccgc
      601 ctgaatcatc ggagcctgaa cgatcggcac atcgattcca agaaggtcac acaacggcgt
      661 tctcaacacc cgatgccatc ccttcgttgc acgcgtcgtt tcgtccggta cccgtgtcag
      721 tccattatct gaccctatca agcggacaaa ccaatgcgct caggaatctc attcttttct
      781 catcatctca tcatgacaac ggcgggcccc acgactatgt tggaggcgtc ggcccgacca
      841 gcatgaagga ggaccagatg cagatccaga tcatcaactt ccagctcagg gagttgtcgg
      901 tcgaggagtt tcacgcggtc tgtgactcga tcgcgccgca atgggcggcc atccccggcc
      961 tgatctcgaa ggtctggctg gagaacgagc agaccaacac ctacggcggt gtctacacct
     1021 gggagagccg tgaggcgctg gagtcgtatc tccagtccga cctgttcaac gggatcatga
     1081 cgaacccgca cttcgccaat gccaccacca ccgacttcga catcatcgaa gggccgacac
     1141 gcgtcacgca tggactcgtt gcggccgctg tctgagttcg gagattcgtg gtactgtacg
     1201 cgtgcatctg atgatatcta cgaatggagc agcgcaatgg cagggtatcc tgatgcgttt
     1261 ggcatctccg taaccgtcga cgacctcaac aaggcacgcc gcttctacac cagcctctat
     1321 ccacacgatc acgtctcaga aggcgtgttc gctggaatca actacatcgg gatcatgcgc
     1381 gacggcgaga cgctcgtgaa tgtcttccag gccgggaaga acaatcccct cgacgacatg
     1441 atccccatcc tcaaggtcga ttcggtcgtg tcctaccagg agacggtccg ggcgctcggc
     1501 ggcgaggttg tgatcccaac tagctcctgc ccatgtacac agacgtcctt cgccgtctgt
     1561 gtcgattcca ccggcaacca gttcatgatc aaggaaccac gctcgtcctg accagtccag
     1621 cgtaatcgaa cctgctcata tcgtcgtcac gccagcatgc aggagacaaa gatggcacgt
     1681 cgtctgggtg atgtcctcgc acaacgcgca tctgagacgt tcgtcggccg aacagatgag
     1741 ttggcagagt tgctgacgat cctggacgac gaccgtccga tcgtcacctg cctgcatggt
     1801 atcggcggca tcggaaaatc cagcctgctg gatgcattca agctccgagc gcgtgacgct
     1861 ggagcaacgg tcatcagtct agacggccgg gtgatcgagc caactcctcg cggcttcctt
     1921 caggaactcg gaatggcgat cggcctcgac gcgacgaccc ccgctctcgc ggtcgatcgc
     1981 cttgaatcgc tcggccagcg cgtcgtgctc acgatcgaca cgtacgagct actgcgcctg
     2041 attgatacct ggctgcggca ggacttctta ccactgctgc cggagaacgt gcgcattgtc
     2101 atctcgggac gtgagccacc agtgtcggcc tggctgtcgt cgcccggctg gcaggacatg
     2161 ttccacagca tcaggctcgg ttcgttatca gaggaagacg ccatcacatt gctccaacag
     2221 cgtggcatcg atgacgtcag cgccctacgt atcaaccgct tcgccgatgg ccatccactc
     2281 gcactgcaac tggcggcggc aaacgttcat gagccatcgc ggctggattt cgcggacgtt
     2341 gctgtccaac gggtcgtcga agagctggca cgcacgtatc tggccgacgt tcccgatccg
     2401 ctg
//
LOCUS       DASUGJ010000427         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_899933, whole genome shotgun sequence.
ACCESSION   DASUGJ010000427 DASUGJ010000000
VERSION     DASUGJ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899933"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1051)
                     /locus_tag="VFV93_09445"
     CDS             complement(47..1051)
                     /locus_tag="VFV93_09445"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165606.1"
                     /translation="MAGKNRVIVIGLGPIGQSVLALVADRPGLVLTGAVDIDPEKVGK
                     DAGIVCGLQQSLGVTVSASLQDALAAGPTDVALLCTGSYLEQVAPQVEAIIGAGLDIV
                     TTCEELAYASVHNPDLAERLDRLARERGVRVLGAGVNPGFAFDALVMTLSATCRRVTA
                     IRATRVLDAGTRRLPLQQKVGAGLSKQEFDALVAAGTVRHVGLLESLNLVADAFGWTL
                     DRTEELTDGVIAERDVTTRYLSVKAGQIAGVKQIGRGYRNGEAVIELELQMYVGAPES
                     FDAIKVEGDPGIDMIIRGGIAGDPVTTALTVNLIGSLEHAPPGLVTMKELSIHYGVGT
                     "
     gene            1093..1224
                     /locus_tag="VFV93_09450"
     CDS             1093..1224
                     /locus_tag="VFV93_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165607.1"
                     /translation="MAGGKKGSASKTIEALKPVVPDDDAGRLFSPEGVAPVAAVLRS"
     gene            complement(1279..>2137)
                     /locus_tag="VFV93_09455"
     CDS             complement(1279..>2137)
                     /locus_tag="VFV93_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165608.1"
                     /translation="PARPSPTPAITPSPAPTPAPTASPTPARSVFADQLVVAYYGNPY
                     SPIMGILGEHDTPELIRRLRIQAARYQALVPDKKVIPALHLVYAVAQANPGDDGSYLY
                     HMPDELVEEYIDVTRREGMLLVIDLQMGRADPVAEVERVRHWLRNEHVHLAIDPEFTM
                     PPGERPGVDLGSMDATTINAIQDVLQTVALEAGTTTNKALIVHQFQADMITNKAAIRR
                     DRERVDVVIDLDGWGPPWSKQEKYQALGTDEPVEYTGFKLFYRWDQPMLSEEQVVALV
                     PRPSVIVYQ"
BASE COUNT          378 a          685 c          685 g          389 t
ORIGIN      
        1 gttgatctgt actccccctt ccctgcgagg gaagggggtc ggggggttag gtcccgacac
       61 cgtaatgaat ggaaagttcc ttcatcgtca ccagtccggg cggcgcgtgc tccaggctgc
      121 cgatcaggtt gaccgtcagc gcggtcgtca ctgggtcgcc ggcgatgccg ccgcggatga
      181 tcatgtcgat gcccgggtcg ccttcgactt tgatcgcgtc gaagctctcc ggcgcgccga
      241 cgtacatctg gagctcaagc tcgatcactg cctcgccgtt gcgatagccg cggccgatct
      301 gtttgacgcc cgcgatctga ccggccttca cgctgaggta ccgggttgtc acgtcgcgct
      361 cggcgatcac gccgtcggtc agttcctccg ttcgatccag cgtccatccg aaggcatcgg
      421 ccaccaggtt gagcgattcg agcaggccaa catgtcgcac cgtgcccgcc gcgaccagcg
      481 cgtcgaactc ctgcttcgaa aggccggctc cgaccttttg ttgcaacggc agacggcgcg
      541 tgccggcgtc cagcacccgc gtcgcacgga tggcggtgac acggcggcag gtggcgctca
      601 gcgtcatcac cagggcatcg aaggcaaatc cagggttcac gccggcaccg agcacgcgca
      661 cgccgcgctc gcgggccagt cggtcgagcc gttccgccaa atccgggttg tgaaccgacg
      721 catacgcgag ttcttcgcag gtggttacaa tgtcgagacc cgcaccgatg atcgcttcga
      781 cctgcggcgc gacctgctcg agatacgagc cggtgcagag gagggcgacg tcggtaggtc
      841 cggccgccag ggcatcctgt agcgatgctg acaccgtgac accgagcgac tgctgcaggc
      901 cgcagacgat gccggcatcc ttcccgactt tctccggatc gatatcgacg gcccctgtca
      961 gtaccaggcc cggacgatcg gcgacgagcg cgagcacgct ctgcccgatc ggtcccagtc
     1021 cgatgacgat gacgcggttc ttaccagcca tccgcaacct ccacgacgcc ggatggtggc
     1081 agtgtatcgg gcgtggccgg agggaaaaag ggaagcgcct cgaagacgat cgaggcgctg
     1141 aaaccggtcg ttccggacga tgacgccggc aggctgttca gccccgaagg cgtcgcgccg
     1201 gtcgctgccg tactccgatc gtaggggagc gtcgtccacc aggacctcac ccaaacgaat
     1261 tagtcgtaga ggggtgtcct actgatagac aataacactc ggtcggggta ccaacgcgac
     1321 tacctgttcc tcggacaaca ttggctgatc ccaccgatag aaaagcttga atcccgtgta
     1381 ctcgaccggt tcatcggtac ccaacgcctg gtacttctcc tgtttactcc acggtggtcc
     1441 ccacccatcc aggtcaataa cgacgtcgac gcgctcgcgg tcgcggcgga ttgccgcctt
     1501 gttcgtgatc atgtccgcct gaaactgatg gacgatgagg gccttgttgg tggtcgtgcc
     1561 cgcttcaagc gccaccgtct ggagcacatc ctggatcgcg ttgatcgttg tggcatccat
     1621 ggagccaagg tcgacgcccg gccgctcacc cggcggcatc gtgaactccg ggtcaatcgc
     1681 aagatggaca tgttcgtttc gcagccagtg acggacccgc tcgacctccg caaccggatc
     1741 ggcccggccc atctggaggt cgatgacgag cagcattccc tcccgtcgag tgacgtcgat
     1801 gtactcctca accagttcgt ccggcatgtg atagaggtaa cttccgtcgt cgccgggatt
     1861 ggcctgagcg acggcgtaga ccaggtgtaa cgcgggaatg accttcttgt ccgggacgag
     1921 cgcctggtag cgggcagcct gaattcgcag ccggcggatc agctccggcg tatcgtgctc
     1981 gccgaggatg cccatgatcg ggctgtaggg gttgccatag tacgccacga ccaactgatc
     2041 ggcgaagacg gaacgcgccg gcgtgggtga cgcggttggc gccggagtag gcgcaggact
     2101 cggagtgatc gctggtgtcg gcgaaggccg ggcaggc
//
LOCUS       DASUGJ010000428         5367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_761359, whole genome shotgun sequence.
ACCESSION   DASUGJ010000428 DASUGJ010000000
VERSION     DASUGJ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761359"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VFV93_09460"
     CDS             <1..1486
                     /locus_tag="VFV93_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165609.1"
                     /translation="SSVVYEYIAEQGTGALERLRNQPGIENTLRIDSKLLAQADFAEG
                     SGNKAVAAEALRRKVSTVGKVGEPGADVRCVVSVGMLTEGWDANNVTQILGLRAFQSQ
                     LLCEQVVGRGLRRLSYEFDLDENGIPTNEEYVDVYGIPFEVIPVKKRAQSTPQPPKPT
                     TLVQALKEREALFAIEFPRVEGYVFSVTDKITCNLNEIEPIDITPSVEPTRVIARPKV
                     GYEVGQPSMHGVGDVVEQSREEFYASIRPQEIQYEIARRLANLLINRDEFALRARHLL
                     FPQVLAIVRAFIATRVQFNGVDPREIGLMRYVDEIVTRLATAIRPASDDGTAKLLPRI
                     ERFRPRGSTAEVAFRTGRETRGTIKSHISHVVLDTATWEASVAVQLETSPLVRAYARN
                     DHLDLTVPYEHGGTQHHFLPDFIVRLTSDTHLLLEVKGYETEQDRQKYEAAKRWCEAV
                     TNWGEMGRWEFRDCPKTTDVQNILAEVSGAQQRLPQPPSLLSSP"
     gene            1811..2317
                     /locus_tag="VFV93_09465"
     CDS             1811..2317
                     /locus_tag="VFV93_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5165610.1"
                     /translation="MDAYLNIVSKRDVRKYRDEPIPDEVLTRILHGGRATGSARNRQQ
                     WEMVVVRSRETLERLAETVAAPANIRGCAAAVAVVLKGQSAWDGGRMAQNLMLAAWSQ
                     GVGTCPNSPVQKDECKAILGVAADADILTILSLGYPAEPVPRASDADAILARIDRKPL
                     EELTRYID"
     gene            complement(2432..2935)
                     /locus_tag="VFV93_09470"
     CDS             complement(2432..2935)
                     /locus_tag="VFV93_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165611.1"
                     /translation="MQATNNTPRQFGLNAFGTTLIALGIAAGIAIGAAGGAIIGSLPT
                     LRTSETAIVLPKAHSSANQGEGMLAGTSAVTAAVRAYTADEQGEGIVGGNLAVAAPLV
                     AHTSLGQGEGIIGGLGSRAALSAPVTAHASAGMGEGWLANGRPATTIKAHASDGQGEG
                     WIANGRP"
     gene            complement(3397..>5367)
                     /locus_tag="VFV93_09475"
     CDS             complement(3397..>5367)
                     /locus_tag="VFV93_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5165612.1"
                     /translation="FEAEQGVNDPAVKQAMEALFATVSAEVTDAQVNSPYAPGGERQI
                     SPDGTIAFAEVNFTDRSQEEYREAGDQIREHAEEIDVSGLQIEFGGNMFIEQADFSSE
                     LIGILCAVVILLIAFGSLLAMGLPIMTALFGIGCGIALIGLAANVLSVPVFASPAAAM
                     IGIGVGIDYALFIVTRYRQGLHDGMEPEAAVVRALDTAGRAVLFAGITVVISLMGMFL
                     INLDAMRGLAISASLAVLMTMLAAVTLLPAVLGFAGRKIDKFGLPHRKQAEGDTRQSI
                     WFRWSRVIQRRPWAAAVIGIVALLILIVPVFSMRLGFGDAGNRPESDTTRQAYDLLSR
                     GFGPGSNGPLLLAVELPNGQQDLPALQGLVDTLNQTDGVAFASPPIPNEAGDTAIVQV
                     IPTTSPQDEATSDLVHHLRDDVIPPVNQANDLDILVGGAPAAVIDFSDYTAGVLPLFV
                     GAVLILSFLLLMAVFRSLVVPLKAVLVNLLSIGAAMGVIVAVFQWGWGLSLIGLGKEG
                     PVEAWAPLMIFAIVFGLSMDYEVFLLSRIREEYDRTHDNATAVADGLAATARVITAAA
                     AIMVCVFGSFVLGSDPALKIMGLGLAVAVLLDATIVRLVIVPALMELLGDRNWWIPGW
                     LDRLLPALNVEPTDEPTTTGERRHDQIPVTGE"
BASE COUNT          961 a         1740 c         1780 g          886 t
ORIGIN      
        1 ctccagcgtc gtctacgaat acatcgccga gcagggaacc ggcgcgctgg aacggctgcg
       61 taatcagccc ggcatcgaaa acacgctccg gatcgactcg aaactgctcg cgcaggccga
      121 ctttgccgag ggttccggca acaaggctgt cgcggccgag gccctgcgtc gcaaagtctc
      181 gacggtcggc aaggtcggcg agccgggcgc ggacgttcgc tgcgtcgtct cggtcggcat
      241 gctgaccgag ggctgggacg ccaacaacgt gacccagatc ctcggcctgc gcgccttcca
      301 gtcgcagctc ctctgtgaac aggtcgtcgg tcgcgggttg cgccgcctgt cctacgagtt
      361 cgatctggac gagaacggca ttccgaccaa cgaggaatac gtcgatgtct acggcattcc
      421 gttcgaggtg attccggtca agaagcgcgc gcagtccacg ccgcagccgc cgaagccaac
      481 cacgctcgtc caggcgttga aggaacgcga ggcgctgttc gcgatcgagt tcccgcgtgt
      541 cgagggctac gtcttctccg tcaccgacaa gatcacttgc aacctgaacg agatcgaacc
      601 gatcgacata acgccgtcag tcgagccgac ccgcgtcatt gcccgcccga aggtcggcta
      661 cgaggtaggg cagccgtcca tgcacggcgt cggcgatgtc gtcgaacagt cgcgcgagga
      721 gttctacgct tcgatccgcc cgcaggagat tcagtacgaa atcgcccgtc gtctcgccaa
      781 cctgctcatc aaccgggacg agttcgccct gcgtgcccgt catctgctct tcccgcaggt
      841 gctggccatc gttcgtgcgt tcatcgcgac gcgcgttcag ttcaacggtg ttgacccgcg
      901 cgagatcgga ctgatgcgct acgttgacga gatcgtgacg cggctcgcga cggcgatccg
      961 tcccgccagc gacgacggaa ccgcgaagct gctcccgcgc atcgagcgct tccgcccgcg
     1021 cggctcgacc gccgaggtcg ccttccgaac cggccgcgag acacgcggga cgatcaagag
     1081 tcacatcagc cacgtcgttc tcgacaccgc cacctgggaa gcgtcggtcg ccgttcagct
     1141 tgaaacgtca ccgctggtgc gggcctacgc gcgcaacgac catctagacc tgaccgtccc
     1201 gtacgagcat ggcggcaccc agcaccactt cctgcccgac ttcatcgtga ggttgaccag
     1261 cgatacccac ctgctgctgg aagtcaaagg ctatgaaacg gagcaggatc gccagaagta
     1321 cgaagccgcc aagcgctggt gcgaagccgt caccaactgg ggcgagatgg gacgctggga
     1381 gttccgcgac tgcccgaaga cgaccgatgt ccagaatatc ctcgccgaag tcagcggcgc
     1441 tcagcaacgg ctgccacaac ctcccagcct gctgtccagc ccgtgacgaa tgtcagccag
     1501 gaaggtcaca ctcacgttgg ccatcttggt catcttggcc accttacccg ctcaagtagc
     1561 tcacacaccg aacttgagct acttgagcca cttgagccac tcacccaatg aacatgagca
     1621 ccttgagcga cttgagcgag atgaccctcc tgaacccact caccagttga ccgtgagtac
     1681 ctttactcct ttggaggccg tggcacgaaa ttccaccgtc agatcgttcg aatagataga
     1741 ggccattccg cgggtcggcg ctattcttac gtgcatggcc gcctggagcg atcgtggacg
     1801 gagacaggga atggacgcgt atctcaacat cgtcagcaag cgcgacgtgc gcaagtaccg
     1861 cgatgagccg atcccggacg aggtgctgac gcggatcctg catggtgggc gggcgaccgg
     1921 cagcgcgcgc aaccggcagc agtgggagat ggtcgtcgtc cggtcgcggg agacgctgga
     1981 gcggctggcc gagacggttg ccgcgccggc caacatccgt ggctgcgcgg cggcggtcgc
     2041 cgtcgtcctg aaggggcagt cggcctggga cggcggccgg atggcgcaga acctgatgct
     2101 ggccgcctgg tcgcagggcg tcggcacctg cccgaactcg cccgtccaga aggacgagtg
     2161 caaggcgatc ctcggcgttg ccgccgacgc cgatatcctg acaatcctct cgctcggtta
     2221 ccccgccgaa ccggttcccc gcgccagcga cgccgacgcg atcctcgccc gcatcgaccg
     2281 caaaccgctt gaggaattga cgcgctatat cgactgaccg atcggtgtac gcgaacagat
     2341 cgacggcgac gcaaaactgg ctaccggacg gggccggtag ccagggggaa gaggaggagt
     2401 tggattcgga tttggatcgc ggttgtccga attacgggcg gccgttggca atccagccct
     2461 caccctgtcc gtccgacgca tgggccttga tggttgtggc tggtcgaccg ttggcgagcc
     2521 agccctcgcc catgccggcg ctggcgtgcg cggtgacggg cgctgacagg gcagcgcggc
     2581 tgccgagacc gccgatgatg ccctcaccct gaccgagcga tgtgtgagcg acgagtggcg
     2641 cggcaacggc cagattgccg ccgacgatgc cttcgccctg ctcgtctgcc gtgtatgccc
     2701 ggacggcggc ggtgacggcg gaggtccctg cgagcattcc ctcgccctgg ttggcgctgc
     2761 tgtgggcctt cgggaggacg atcgcggtct cgctcgtccg cagggttggg aggctgccga
     2821 tgatcgctcc acctgccgcg ccgatcgcga tgccggccgc gatgccgagg gcgatcaggg
     2881 tcgttccgaa tgcgttgagg ccgaattgcc ggggagtgtt gttggttgcc tgcattgtct
     2941 ggctcccttc gacgttgtcc atcagtgcgt tgtcgatgga tgcagaatag ggagttccag
     3001 acggcgcaac atcgtgcaga tgacgtgact ttcctcaggg gtctacacgc agcggagtac
     3061 gttatctaac gtacccgtcg ccagcgtcat gtcaggccgt gctggacggc aacgcgcgtc
     3121 gcggccgcgc gggatgtgcg caactgtgct gcccaagttg ggcattcggg gtcaggcagg
     3181 cgtcgtcgag gaggagtgac tcgccgaggg acattgtccg gcttcgtcgg ttcgctcatt
     3241 ccctgatcct ctcgaaatcg aacctgaaca tttctgatgg ccgggcgtgg tgacgatgat
     3301 tgtcgtctga ccaccagcgg tcaacctgct ccaatacgcg aatatgaccc tatgaagcag
     3361 gttgacggct gaacgctggc ggcgattgac tgattgctac tcccctgtaa ccgggatctg
     3421 atcgtggcgg cgttcgccgg tcgtcgttgg ttcgtcggtt ggttcgacgt tgagcgccgg
     3481 aagcagccgg tcgagccagc cggggatcca ccagttgcgg tcgccgagca gctccatcag
     3541 ggccgggacg atcaccagac ggacgatcgt cgcatccagc aggacggcga cggccaggcc
     3601 gagccccatg atcttgaggg ccgggtcgga gcccagcacg aagctgccga agacacagac
     3661 catgatcgcg gcggcggccg tgatgacgcg tgccgtcgcg gccagcccgt cggcaacggc
     3721 cgtcgcgttg tcgtgggtgc ggtcgtactc ctcgcggatg cgcgagagca ggaagacctc
     3781 gtagtccatc gagaggccga agacgatcgc gaagatcatc agcggcgccc aggcttcgac
     3841 cggtccttcc ttgcccagcc cgatcaggct cagcccccag ccccactgga agacggcgac
     3901 gatgacgccc atcgccgcgc cgatcgagag caggttgacc agcaccgcct tgagcgggac
     3961 gaccaggctg cggaagaccg ccatcagcag caggaacgag aggatcagga cggcgccgac
     4021 gaacagcggc agcacgccgg ccgtgtagtc ggagaagtcg atgacggccg ccggcgcgcc
     4081 gccgaccagg atgtcgaggt cgtttgcctg attgaccggc gggatgacat cgtcgcgcag
     4141 gtggtggacc aggtcggagg tcgcttcgtc ctgcggtgag gtggtcggga tgacctggac
     4201 gatggccgta tcgccggcct cgttggggat cggcgggctg gcgaaggcaa ccccgtcggt
     4261 ctggttgagc gtatcgacca gtccctgcaa ggccggcaga tcctgctggc cgttgggtag
     4321 ttcgacggcc agcaatagcg gcccgttcga gccggggccg aagccgcgcg agagcaggtc
     4381 gtacgcctgg cgggtcgtgt ccgactccgg ccggttgccg gcgtcgccga agccgagccg
     4441 catcgagaag accggcacga tcaggatcag cagcgcgacg atgccgatga cggcggccgc
     4501 ccacggacgg cgctggatga cgcggctcca gcggaaccag atcgattggc gcgtgtcgcc
     4561 ctcggcctgc ttgcggtgcg gcaggccgaa cttgtcgatc ttgcggccgg cgaaaccgag
     4621 gacggccggc agcagcgtga cggcggccag catcgtcatc aggaccgcca gcgaggcgct
     4681 gatcgccagc ccgcgcatcg cgtcgaggtt gatcaggaac atccccatca acgagatgac
     4741 gaccgtgata ccggcgaaga ggacggcccg gccggctgtg tcgagcgcgc ggacgacggc
     4801 tgcctccggc tccatcccgt cgtgcagccc ctggcggtag cgggtaacga tgaacagcgc
     4861 gtagtcgatc ccgacaccga tgccgatcat cgccgccgcc ggtgaggcga acaccggcac
     4921 cgagaggacg ttggcggcca gcccgatcag cgcgatcccg cagccgatcc cgaacagcgc
     4981 cgtcatgatc ggcagcccca tcgccagcag cgagccgaag gcgatcagga gaatcacgac
     5041 cgcgcagaga ataccgatca gctcgctgga gaaatcggcc tgctcaatga acatgttgcc
     5101 gccgaactcg atctgcaggc cggagacatc gatctcctcg gcgtgctcgc gaatctggtc
     5161 gccggcctcg cgatactcct cctggctgcg gtcagtgaag ttcacctcgg cgaacgcgat
     5221 cgtgccatcc ggcgagatct gccgctcgcc gccgggcgcg taggggctgt tgacctgcgc
     5281 gtcggtgacc tcagcgctga cggtcgcgaa gagcgcctcc atcgcctgtt tgacggccgg
     5341 atcgttgaca ccctgctcgg cctcgaa
//
LOCUS       DASUGJ010000429         8553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1454808, whole genome shotgun sequence.
ACCESSION   DASUGJ010000429 DASUGJ010000000
VERSION     DASUGJ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454808"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..2101)
                     /locus_tag="VFV93_09480"
     CDS             complement(125..2101)
                     /locus_tag="VFV93_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEX5165613.1"
                     /translation="MVLTASAATTYEAAEPALLGEVDRSSSAYVRWVQSSLNQIMSAG
                     LAVDGVSGPLTRAAIRSFQTSRGLTADGIVGPITEAALIAAGAPQPPQVGGGGGTTTG
                     PHAAVSARLPSPGAGYYSYKPGSNQYGLPETIQALQAIGSQWHRAHPSGPRIGIGDIS
                     LQGGGRMSGHASHQLGVDIDIRIVRNDGREEPANYTLSTYSRTLTQELVNTIRANGVL
                     KVQYIFFNDSKVTGVQPWPNHDDHVHVRFFPPGSSSERELEEALGEVNRSSAEYIRWV
                     QTSLNRLQGAGLAVDGISGPLTRAAVRTFQASRGLAVDGIVGPITEGALMSAGADPPP
                     GWVPPPLDPLTTTETLALAELTPDQKALFLQLRLTIEGYTGVARREGDKGSRVLLTRG
                     SYKVLPQLLPELVRLTGITTLPSGWSLTGARPLLVGNVLYNLAFPETINQGGSDRLGG
                     KPDPTCFSASTQMLLARRFPVTYVRLTIELATTNRATFAGGASIGPLTFKSTNLYRSL
                     ESVLLQTAFDTYFTTVAISGSYTPGDELKVHRQVFGATRPPANATWGTKATMVAAFRK
                     AFITNGGTTRPFEIINLCVGATTPSCLGNHTVVLSRVLNGRVYFYNPWANEEERNMMW
                     GAAQVSTSGNGEHPAESSMTQADFEGQLTTVFHN"
     gene            complement(2225..3370)
                     /locus_tag="VFV93_09485"
     CDS             complement(2225..3370)
                     /locus_tag="VFV93_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165614.1"
                     /translation="MPTMKELQERRDRLALARATVMDEREAELIENPQLAEALQHEID
                     DIEQKLAEADAKQPRPVDGGLADLIGIGAGAPADISGVRQPSKIEEYDDEVSSERLLA
                     VADLYYIYQMERLGVFTVILKLQDLFKAGTVRLSAGQGANALYRYDRKQVLRYTRKDR
                     LQAYRRVFGYGNVPPVPGAQPNHQFHALFVNFMTQVAEFFRDKRVSEVIRPRADDPSF
                     GSIAVVRRAGLDLRNNLDSASYGHINVLRVEVMQLLDDAFVILESDDIKRLFGASDGW
                     DVIEEVMRQHLNQPQINASQRNRMGIAGSEVLQWLSHPYILKTSRAEFEGLLLDIGDR
                     AEEWVTSAQALGMVQRPGRSGSLPWEGGRMAVSANGRSNGNGRYMGG"
     gene            complement(3432..5255)
                     /locus_tag="VFV93_09490"
     CDS             complement(3432..5255)
                     /locus_tag="VFV93_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165615.1"
                     /translation="MALANTGCIQGKVVLRIPLGDASDPARHTTFPLPGVTVTLSSAG
                     KGARSAGSRTVQTDAYGEFCIDNLPSGMYTVWYPDKVIRDGSAFPAYNASLSEDVDAP
                     LGSSCAAGDCGLATTFAYVVAVSPQAAAVTASAQGIDAAMQTTAASTYGIGQILSDAS
                     QAVADHHGIIDATMTSVLGIDATLSGTATDINAIAQTLSDATQGINNHQQLIKDTANA
                     VQDISTALQAMSVFPYAGPTESNGYHPQPPAGEKRTPVAATAGIRGGVQAALADIGLT
                     GDITDAGLASLFPSETDGAGDVTYKWAGPAVATPTTVRSNGARQPGEVKVSGGLALFQ
                     QQAQAALREVRNAFSSIKPINSNVATPAAIEAQKAIVLDTIQSIVDEPDRPEGLRALA
                     VDAHFDTLLTDTIVVSGNSFGGKVTRTGNLSALEYMLGFSTVVITDQDAEAHATEWQR
                     AEDAVLSLKDSWDAFRKKKDLKLGERLQDLDGYFEALSEAADSARSMLKTVRFSAQEQ
                     GATQFPATPGGPDEVTTDDVLSMVDELAERIGPKYITDWKGRGIGTIEGRARKLKVTV
                     TAVNASNKFPFDNARVKSAFNDIVRLLASIEALCKQINAAI"
     gene            complement(5321..5923)
                     /locus_tag="VFV93_09495"
     CDS             complement(5321..5923)
                     /locus_tag="VFV93_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165616.1"
                     /translation="MTVSSAFTELEQEFRQLERVAGNVNWAVVQVRPASTDGQSIADY
                     YESATIDFGALAKAARQAADDGRRATTAQADLFTLRRALVDCQEYANQLVQRYYGDIA
                     SCERLANLNDLARRGSRDWATWVIGVRDGLSSCPQILHSISQSLLHCWQEITEPIGPM
                     SVTAQATSLGSRIVIRGSPEINESPAEYDGDTSINTTLSR"
     gene            complement(5928..7265)
                     /locus_tag="VFV93_09500"
     CDS             complement(5928..7265)
                     /locus_tag="VFV93_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165617.1"
                     /translation="MVTTVRPIALSSEPTPARPVLGEPANAPAAALDDFLRAQAVNVS
                     QHAAALRPFRADEFGSGPEAPSSAHIQAANDLIDRMRQSLLALSQLVQRSAQMSAAAP
                     DSRSFNDLLTQKDRAGDRVKSVERVWDFYLELFGQRQSRYAGWLLGADRIAIDCYQAV
                     YTGLGRAQPVPSPLPFAFMATGFTPATFRRGVPLTKLVQQNNPFPIVEIPFHRLVNPW
                     TLGAIHHEVSHNLQNDLGLWTEVPRRIGRRLRQAGIDSKVAMTWSTWNKEIWADLCGL
                     LLGGPAVVASLIDVVAVAPSRALSFNPTGVHPTPYLRTLINLELLNRMGFTDEAAAYR
                     ALWDRLYPDPRGGHLPAAIMDSFDEAHRIVVDEICYTPYARLGNKRLAEVVSFNTTHD
                     AMTREAGERLARGADPGVVPVRFLVGAARWALDRRLATPYALTKSFYDALVRR"
     gene            complement(7430..>8553)
                     /locus_tag="VFV93_09505"
     CDS             complement(7430..>8553)
                     /locus_tag="VFV93_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165618.1"
                     /translation="RHAYRQRSWIMWEPETLEMQELGENEGFDFEGSGENDFFGDFEG
                     ASWESGSDLFSDYESVSGASLEDLEGDPFLGDLVRGATQTLNRVTGGIVNDQFLKNLA
                     KQAATVAGGAIAGQQGARIAGQLANQVIREGDFESMYENGSYEGDLMSAGVDMEALAD
                     LHYYANQMAEAQSEAEADQFLGALLPVIGQVAAPLISNLLGGRRESGDFFDEEDWSGE
                     SRDEFLPALIPLAAPLIAKGVGAIGKMLSKGPKTRKFAAAAPKIATAAATKVARQAAS
                     GRPPTPQRIAATVGSEAARVLSSPTQLKKTVQQNRAIARRVSSGVPAASAVATRPNGN
                     GARPVPRPHGRAPYGAYGASGATRGRPRVIGYLPVYAKR"
BASE COUNT         1270 a         2803 c         2960 g         1520 t
ORIGIN      
        1 cgaacccacc cggcccgctc aaacccgcga ggagccatga acccgcttgc tcgcggcgct
       61 gcgtcaagcg atccagcgcg ttcacggctc ccacgcgtcc cgcgtcccgc gtcccaccac
      121 ccggtcagtt atggaagacg gtcgtcagct ggccctcgaa gtcggcctgc gtcatgctcg
      181 attcggcggg gtgctcgccg ttgccggagg tcgagacctg ggctgcgccc cacatcatgt
      241 tgcgctcctc ttcgttggcc cacgggttgt agaagtagac gcggccattc aggacgcggc
      301 tgaggacgac cgtgtggttg ccgagacaac tcggtgtcgt cgccccgacg cagaggttga
      361 tgatctcgaa cgggcgtgtc gtaccgccgt tggtgatgaa tgccttgcgg aaggcggcga
      421 ccatcgtcgc cttcgtcccc caggtcgcgt tggccggcgg gcgagttgcg ccgaagacct
      481 ggcggtggac cttgagttcg tcgccgggcg tgtagctgcc gctgatcgcg accgtcgtga
      541 agtaggtgtc aaacgccgtc tggagcagga cgctctccag gctgcgatac aggttggtgg
      601 acttgaacgt gagcgggccg atgctggcgc cgccggcgaa ggtcgcccgg ttcgtcgtcg
      661 caagctcgat cgtcagccgg acatacgtga ccgggaagcg gcgcgccagc agcatctggg
      721 tcgaggctga gaagcaggtc gggtcgggct tgccacccag ccggtcgctg ccgccctggt
      781 tgatcgtctc cgggaaggcc agattgtaga gcacgttgcc gaccagcagc ggccgtgcgc
      841 cggtcaggct ccagccgctg ggcagcgtcg tgatcccggt cagcctgacc agttccggca
      901 gcagttgcgg tagcactttg tagctgccgc gcgtcagcag gacgcggctg cctttgtcgc
      961 cctcacgccg ggcgacgccg gtgtagccct cgatcgtcag gcgcagttgc aggaagagcg
     1021 ccttctgatc gggcgtcagt tcggccagcg ccagcgtctc ggtcgtcgtc agcgggtcga
     1081 gcggtggcgg cacccagcca ggcggcggat cggcgccggc tgacatcagc gcgccctcgg
     1141 tgatcggccc gacgatcccg tcgacggcca gcccccggct cgcctggaac gtgcggacgg
     1201 ccgcgcgggt cagcggcccg ctgatcccat cgacggccag ccccgcgccc tggagccggt
     1261 tcagcgatgt ctggacccag cggatgtact cggcgctgct gcggttgact tcgccgagcg
     1321 cctcttcgag ctcgcgctcg ctggacgagc cgggcgggaa gaagcggacg tgtacgtggt
     1381 cgtcgtggtt cggccacggc tgaacgccgg tgaccttcga gtcgttgaag aagatgtact
     1441 gcaccttgag gacgccgttg gcgcggatcg tgttgaccag ttcctgtgtc agcgtccgcg
     1501 agtaggtcga gagcgtgtag ttggccggct cctcgcggcc gtcgttacgg acgatgcgga
     1561 tgtcgatgtc gacgccgagc tggtgcgatg cgtggccgga catccgaccg ccgccttgca
     1621 ggctgatgtc gccgatcccg atccgcggac cggacggatg cgcccggtgc cactggctgc
     1681 cgatcgcctg gagggcctga atcgtctccg gcagaccata ctgattgctg cccggcttgt
     1741 agctgtagta gcccgcgccg ggcgagggca gccgcgccga gacggcggcg tgcggcccgg
     1801 tcgtcgtgcc gccgccaccg ccgacctgtg gcggctgggg cgcgccggcc gcgatcagcg
     1861 ccgcttcggt gatcggcccg acgatgccgt cggcggtcag gccgcggctg gtctgaaacg
     1921 agcggatcgc cgcccgcgtc agcggcccgc tgacgccatc gacggccagg ccggcgctca
     1981 tgatctggtt gagtgagctc tgcacccaac ggacgtaggc gctgctgctg cggtccacct
     2041 cacccagcag ggcaggttcg gccgcttcgt atgtcgttgc cgcgctggct gtgagtacca
     2101 tggcgcgctc ctcttcgaga cagcaaagcg atgggtttca acgacggata gctcagggcc
     2161 gcaggacaaa ccagccggca cgtgagtcgg accatcgcgc cgcgagggtg atcgccgccg
     2221 gcgatcaccc gcccatgtag cggccgttgc cgttgctgcg gccgttcgcg gagacggcca
     2281 tccggccgcc ctcccacggc aacgaaccgc ttcggccagg ccgctggacc atgccgagcg
     2341 cctgggcgct ggtgacccac tcctcggcgc ggtcgccgat gtcgagcagc aacccctcga
     2401 actcggcccg cgaggtcttc agaatgtacg ggtgtgacag ccattgcagc acctcgctgc
     2461 cggcgatgcc catccggttg cgttgcgagg cgttgatctg cggctggttc aggtgctgcc
     2521 gcatgacctc ttcgatgaca tcccaaccgt cgctggcgcc gaacaggcgc ttgatgtcgt
     2581 ccgattcgag gatcacgaag gcgtcgtcga gcaactgcat aacctcgacc cgcaggacgt
     2641 tgatgtggcc gtacgaggcc gagtcgaggt tgttgcgcag gtcgagcccg gcccgccgga
     2701 cgacggcgat gctgccgaag ctcggatcat cggcgcgcgg ccggatgacc tcggagacgc
     2761 gcttgtcacg gaagaactcg gcgacctgcg tcatgaagtt gacgaacagg gcgtggaact
     2821 gatggttggg ctgcgctccc ggcaccggtg gcacgttccc gtagccaaag acgcgccggt
     2881 aggcttgcag gcggtccttg cgcgtgtagc gcagcacctg tttgcggtca taccgataca
     2941 gcgcgttggc accctgtccc gccgacaggc ggaccgtccc agccttgaac agatcctgga
     3001 gcttgagaat gacggtgaag actccgagcc gctccatctg atagatgtag tacaggtcgg
     3061 cgacagccag cagccgctcc gacgagacct cgtcgtcgta ctcctcgatc ttggacggct
     3121 gcctgacgcc gctgatgtcg gccggcgcgc cggctccgat gccgatcagg tcggccagcc
     3181 cgccgtcgac cggtcggggc tgcttcgcgt ctgcctcggc cagcttctgc tcgatgtcat
     3241 cgatctcgtg ctgcagcgcc tcggcaagct gcgggttctc gatcagttcg gcctcgcgct
     3301 cgtccatcac ggtggcgcgc gccagcgcca ggcggtcgcg ccgttcctgc agctctttca
     3361 tggttggcat gacggtcctc ctgccgcttg cgtccgatcg gttgcacgct gcctcccgaa
     3421 cgcggctgat actagatggc agcgttgatc tgcttgcaga gggcttcgat gctcgccagc
     3481 agtcgcacga tgtcgttgaa ggcgctcttg acgcgcgcgt tgtcgaacgg gaacttgttg
     3541 ctggcgttga cggcggtaac ggtcaccttc agcttgcgtg cccggccctc gatcgtgccg
     3601 atgccacgcc ccttccagtc ggtgatgtac ttcgggccga tccgctcggc caactcgtcg
     3661 accatcgaga gcacgtcgtc ggtcgtgacc tcgtccggcc cgcccggcgt ggccgggaac
     3721 tgggttgcgc cctgttcctg ggccgagaag cgcaccgttt tcagcatcga ccgcgcgctg
     3781 tcggctgcct cggagagtgc ttcgaagtag ccgtccagat cctggagccg ctcgccgagc
     3841 ttgagatcct tctttttgcg gaaggcatcc caggagtcct tgagcgagag aacggcatcc
     3901 tcggcgcgct gccactcggt cgcgtgtgcc tcagcgtcct gatcggtgat gacgactgtc
     3961 gagaagccga gcatgtactc cagtgcgctg aggttgccgg tgcgcgtcac cttgccaccg
     4021 aatgagttgc cggagacgac gatcgtgtcg gtcagcaagg tatcgaaatg ggcgtcgacc
     4081 gcgagggccc gcaacccttc cggacgatcc ggctcgtcaa cgatgctctg gattgtgtcc
     4141 agcacaatcg ctttctgcgc ctcgatcgcg gctggagttg cgacgttgct gttgatcggt
     4201 ttgatggacg agaacgcgtt gcgcacctcg cgcagcgccg cctgtgcctg ctgctggaag
     4261 agcgccagcc cgccggagac cttgacctct cccggttggc gcgcgccgtt tgagcggacg
     4321 gttgtcggcg tcgcgacggc cggccccgcc cacttgtagg tcacgtctcc ggcaccgtcg
     4381 gtctcggatg ggaacagact cgccagcccc gcgtcggtga tgtcgccggt cagcccgatg
     4441 tcggccagcg ccgcctggac gccgccccgg ataccggccg tggccgcgac tggcgtccgt
     4501 ttctcgccgg ccggcggctg cgggtggtag ccgttggact cggtcggccc cgcatagggg
     4561 aagaccgaca tcgcctgcaa cgcggtactg atgtcctgga cggcgttggc cgtgtccttg
     4621 atgagctgct ggtggttgtt gatcccctgc gtcgcgtctg acagtgtttg cgcgatcgcg
     4681 ttgatgtcgg tcgcggtccc gctcaaggtg gcgtcgatgc cgaggaccga ggtcatcgtc
     4741 gcgtcgatga tgccgtgatg atcggcgacg gcctgcgacg cgtcggacag gatctgcccg
     4801 ataccgtagg tgctggcagc ggtcgtctgc atggcggcgt cgatcccctg ggcgctggca
     4861 gtgacggccg ctgcttgcgg tgagacagcc acgacgtagg cgaaggtcgt cgccaggccg
     4921 cagtcaccgg ccgcgcacga gcttccgagc ggcgcgtcga catcctcact gaggctcgcg
     4981 ttgtacgctg ggaaggcgga cccgtcgcgg atgaccttgt cgggatacca gacggtgtac
     5041 atccccgacg ggaggttgtc gatgcagaat tcgccgtagg catcggtctg gacagtcctc
     5101 gagcccgccg atcgtgctcc cttgcctgca ctgctcaacg tcacggtgac gcccggcagc
     5161 gggaacgtcg tgtgccgggc agggtcactc gcgtcaccga gtgggatgcg cagaacgacc
     5221 ttcccctgaa tgcagccagt gttggcgagt gccatgctgt gcctccttga ctaccgcgag
     5281 tgacgactgg aacggacccg cgaccaccag aaacggctgg tcatcgcgag agcgtcgtgt
     5341 tgatggacgt gtctccgtcg tattcagccg gagactcgtt gatttcaggt gaaccgcgaa
     5401 taacgatgcg ggagccgagg ctggtcgcct gggccgtgac ggacattggg ccgatcggct
     5461 cggtgatctc ctgccagcag tgcaggagtg actgactgat cgagtggagg atctgcgggc
     5521 aactgctcag gccgtcgcga acgccgatga cccaggttgc ccaatcacgg ctgccgcgcc
     5581 gcgccagatc gttgagattg gcgagacgtt cacacgaagc gatgtcgcca taatagcgct
     5641 gcacgagttg atttgcatac tcctgacagt cgaccagcgc ccgccggagt gtgaagagat
     5701 ctgcctgcgc cgtcgtcgcg cggcgtccat cgtccgccgc ctgccgggcc gccttggcga
     5761 gcgcgccgaa gtcaatcgtc gccgactcgt agtagtcggc aatcgattgt ccgtcggtcg
     5821 acgcgggtcg aacctggacg acggcccagt tgacgttgcc ggcgacgcgc tcaagctgtc
     5881 tgaattcctg ttccagttcg gtgaacgctg atgaaaccgt catcgcgtca tctcctgacc
     5941 agcgcgtcgt agaacgactt ggtcaacgca tagggcgtcg cgagccggcg atcgagtgcc
     6001 cagcgggcgg ccccaaccag gaagcggacc gggacgacac cggggtcagc gccacgggcg
     6061 agccgctcac cagcctcgcg cgtcatcgcg tcgtgggtcg tgttgaacga gacgacctcg
     6121 gccaggcgct tgttgccgag ccgggcgtag ggcgtgtagc agatctcatc gacgacgatc
     6181 cggtgggcct cgtcgaagct gtccatgatc gccgccggca ggtgtccgcc gcgcggatcg
     6241 ggatagagcc gatcccacaa ggcgcggtat gcggctgcct catcggtgaa gcccattcgg
     6301 ttcagcagct ccaggttgat cagcgtccgc agatacggag tcgggtggac gccggttggg
     6361 ttgaacgaca gcgcccggga cggcgcgacg gcgacgacat cgatcagcga ggcgacgacg
     6421 gccgggccgc cgagcagcag gccgcagagg tcggcccaga tctccttgtt ccaggtcgac
     6481 caggtcatcg ccaccttcga gtcgatgcct gcctgccgca ggcggcggcc gatccggcgc
     6541 gggacttcgg tccacagccc caggtcgttc tgcaggttgt gcgagacctc gtggtggatg
     6601 gcccccagtg tccaggggtt gaccagccgg tggaatggga tttccacaat cgggaacggg
     6661 ttgttctgct ggaccagctt cgtcaacggc acaccgcgcc ggaaggtcgc cggcgtgaac
     6721 cccgtcgcca tgaacgcgaa cggcagcggc gagggaaccg gctgcgcacg gcccaggccg
     6781 gtgtagaccg cctggtagca gtcgatcgcg atccggtccg cacccagcag ccagccggcg
     6841 taccgtgact gccgctggcc gaagagttcg agatagaagt cccagacgcg ctcgacggac
     6901 ttgacccggt cgccggcccg gtccttctgg gtcagcaggt cgttgaagga ccggctgtcg
     6961 ggggcggcag cgctcatctg tgccgagcgc tgcacgagct gtgacagcgc cagcagcgac
     7021 tggcgcatcc ggtcgatcag gtcgttggcg gcctggatat gcgcgctcga cggcgcttcc
     7081 ggcccgctgc cgaactcgtc ggcccgaaac ggcctgagcg ccgccgcgtg ctgggagacg
     7141 ttgacggcct gggcgcggag gaagtcgtcg agcgccgcag ccggagcgtt cgccggctcg
     7201 ccgagcaccg gtctggctgg tgtcggttca ctggagagcg caatggggcg gacggtcgtg
     7261 accatggcaa cccatcctgt cggccggcaa gctggcgctg agtgcgtgaa cgtggcagtc
     7321 gatggccggt cggggctggt ggctcgtggt tactggagct gtgggaggga ggtaaccacg
     7381 agcctgccgc gaccggccgg gcgttgcatc gggggaaggt cgggcccggt cagcgcttgg
     7441 cgtagaccgg caggtagccg atcacgcgcg ggcgtccacg ggtggcgccg ctggcgccgt
     7501 atgcgccata cggcgcccgc ccatgcggcc ggggaactgg gcgcgcaccg tttccattgg
     7561 gtcgcgtcgc aacagccgag gccgccggga cgccggacga cacgcgtcgc gcgatcgccc
     7621 ggttctgctg gaccgtcttc ttgagctgcg tcgggctgga cagcaccctg gctgcctcgc
     7681 tgccgacggt cgcggcgatc cgctgcggtg tcggtggccg gccgctggcg gcctggcgcg
     7741 cgaccttggt cgcggctgcc gttgcgatct tcggggcggc ggcggcgaac ttgcgcgtct
     7801 tcggcccctt ggacagcatc ttgccgatcg cgccgacgcc tttggcgatt aacggcgccg
     7861 ccagcgggat cagggccggc aggaactcgt cgcgcgactc cccgctccag tcttcctcgt
     7921 cgaagaagtc accggactcc cggcgaccgc ccagcaggtt cgagatcagg ggcgcggcca
     7981 cctggccgat caccggcaac agcgcgccga ggaactgatc ggcctcggcc tcgctctgcg
     8041 cttcggccat ctggttggcg tagtagtgca ggtccgcgag tgcttccata tcgacgccgg
     8101 cgctcatcag gtcgccctcg tagctgccgt tctcgtacat cgactcgaag tcgccctcac
     8161 ggatgacctg gttggcgagc tggccggcga tcctggcgcc ctgctggccg gcgatcgcgc
     8221 caccggcgac ggtcgcggcc tgtttggcca ggttcttcaa gaactggtcg ttgacgatgc
     8281 cgccggtgac gcggttcagc gtctgggtgg cgccacggac gaggtctccg aggaacggat
     8341 cgccctcgag atcctccagg gaggcccccg agaccgactc atagtcgctg aacaggtctg
     8401 agccgctctc ccaggatgcg ccctcaaagt cgccgaagaa atcgttctct ccggaccctt
     8461 cgaagtcgaa gccttcgttc tcgcccagtt cctgcatctc caacgtctca ggctcccaca
     8521 tgatccagct cctttgtctg tacgcgtggc gag
//
LOCUS       DASUGJ010000430         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1039625, whole genome shotgun sequence.
ACCESSION   DASUGJ010000430 DASUGJ010000000
VERSION     DASUGJ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039625"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFV93_09510"
     CDS             <1..722
                     /locus_tag="VFV93_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165619.1"
                     /translation="FLVDLDRNYYFLEMNTRLQVEHPVTEIVAGVDLVADQIRVAAGE
                     SLAYTQETVPRRGWAIECRIVAEDPRQNFIPSVGTIAFAREPAGPGVRVESALYDGFE
                     VSIYYDALVAKVTAWGRNREEAIRRMRRALSEFKIVGVETNLQFHVQVMDNPFFRAGQ
                     IDTDFLVKHFDPNYEPPQEGEDAALIAAALLSHMRMTGVRVNEVEAGTDTNGVGATVN
                     PWRNMARPGRMGGAGWRGSMR"
     gene            701..1198
                     /locus_tag="VFV93_09515"
     CDS             701..1198
                     /locus_tag="VFV93_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5165620.1"
                     /translation="MARKYALRVDDRQEEAEVLEEDGILRVRLGERWHTAELERTNRQ
                     GLYSLIIDGHSWELFARERPGVFELLVGNRLYEVAVGSGRRTADSEEGVTGVWSLQSP
                     MSGQVIEVRVEAGESVQAGQTLVVVESMKMNNELTAARAGTVSEVQVNPGDRVERGRL
                     LLRVS"
     gene            1308..1883
                     /gene="infC"
                     /locus_tag="VFV93_09520"
     CDS             1308..1883
                     /gene="infC"
                     /locus_tag="VFV93_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5165621.1"
                     /translation="MNDRIRVREVRLIDDEEGQVGIVPVQQALQMARDKGLDLVEVAP
                     NAVPPVCRILDYGKFKYEQSKKEREARKHQHNVVLREIRMKPKIDDHDVDFKTRTVQK
                     LLKEGDKVKVSVMFRGREVTHPQIGRALLDRIYERVKEHGTLEKQPSMEGRFMTMILA
                     PGQPKAAKDAPKPRSQAQPDQELVTAPVGEQ"
     gene            1897..2088
                     /locus_tag="VFV93_09525"
     CDS             1897..2088
                     /locus_tag="VFV93_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5165622.1"
                     /translation="MKTSSAAKRRFSVTGTGKVMRMKGNKRHKKMAKSRRVLQMDEHK
                     FPVASTHRRILQRLLPYGA"
     gene            2160..2516
                     /gene="rplT"
                     /locus_tag="VFV93_09530"
     CDS             2160..2516
                     /gene="rplT"
                     /locus_tag="VFV93_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5165623.1"
                     /translation="MPRVKRGVTGHARHKKIIEMAKGHKGQRHRLFRRANESVTHALS
                     YAYRDRRDRKSQFRRLWILRINAAARQHGLSYSRLIDGLAKAGVVLDRKVLADIAVRD
                     QAAFGKLVEQAKAGTA"
     gene            2617..3975
                     /locus_tag="VFV93_09535"
     CDS             2617..3975
                     /locus_tag="VFV93_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5165624.1"
                     /translation="MNEGWLTAAKLLAVVALVLANGFFVASEFALVAIRRSRVDEMVS
                     QGVLGAKGVQRATRNLDHFIAATQLGITLASLALGWLGEPTLGRLLEHRIGHVPGGTT
                     LAVIIAFSIITTLHIVVGELAPKSIALQYPERTSLVIAQPLGIFDFVFRPFIIALNSI
                     GRFTVRMIGLQPPSGHELVHSSEELRILVEASGRAGVLEESEREIINRAFDFADFTAR
                     ELMMPRTEFVAVPADLSGRDLLHTVVEKGFSRYPVYQGSLDDILGVVHVKDLVAAMDR
                     DSLENITAKDLARPAMIVPDTLPVDDLLDRLRDTNARMALVIDEFGGVAGIVTMENVL
                     ERLVGQLRDEFEPQSQTEVVHHPDGSVSISGLTLISDANELLGLELDDAEYDTVGGFL
                     FGHIGHLPQVGDSVEVDGHALRVESMDDRRVDRVLVTSNGPNAPAVTLRGRNGLEQPG
                     DS"
BASE COUNT          830 a         1178 c         1375 g          762 t
ORIGIN      
        1 agttcctcgt cgacctcgat cggaattact acttcctgga gatgaacacc cgtcttcagg
       61 tcgaacatcc agtgacggag atcgtcgccg gtgtggattt ggtggccgat cagatccgtg
      121 tcgctgccgg agagtcgctc gcctatacgc aggagacagt gccgcgccgt ggttgggcga
      181 tcgagtgccg aatcgtcgcc gaagatccgc gtcagaattt catcccatcg gtagggacga
      241 tcgccttcgc ccgcgagccg gccgggccgg gcgtgcgcgt cgaaagcgcg ttgtacgacg
      301 gcttcgaggt ctcgatctac tatgacgcgc ttgtcgcgaa agtgaccgct tggggtcgca
      361 atcgagagga ggcgatccgg cgcatgcgaa gggcgctgtc ggagttcaag atcgtgggag
      421 tggagacaaa tctgcaattc cacgtgcagg taatggataa tccgttcttt cgcgccggtc
      481 agatcgacac ggacttcctg gtgaagcact tcgatcccaa ttacgagccg ccgcaggagg
      541 gagaggatgc cgcgctgatt gcggcggcgt tgctctcgca catgcggatg actggtgtac
      601 gagtcaatga agtggaggcc gggacggata ccaacggtgt tggagcgacg gtcaatccgt
      661 ggcgcaacat ggcgcgtccg ggacggatgg gaggtgccgg atggcgcgga agtatgcgtt
      721 gagggttgac gatcggcagg aagaggccga agtcctcgag gaggacggca ttctgcgggt
      781 ccggctcggc gaacgatggc atacggccga gctggaacgg accaaccggc agggcctcta
      841 ttcgctgatc atcgacggcc attcgtggga gctttttgcg cgggagcggc ccggcgtgtt
      901 cgagttgctc gtcggcaacc ggctctatga ggtggcagtg ggcagcgggc ggcggacggc
      961 cgactcggag gaaggggtca ccggcgtttg gagtctgcaa tcgccgatgt cgggccaggt
     1021 gattgaagtt cgggttgaag ccggggaatc agtacaggcc ggacagacgc tcgtcgtggt
     1081 cgagtcgatg aagatgaaca acgagctgac ggcggcgcgg gccgggacgg tgagcgaggt
     1141 tcaggtgaat cccggcgatc gggtggagcg tggccggctg ctcctgcgcg tgagctaatc
     1201 acgttgccga tccgcgcggt tccatggtag actgagacgt tacagggcgt atgctcggag
     1261 caaccgagcc aagtgggagg tagtcttcta tcacacgaga gtttcgtatc aacgaccgta
     1321 tccgcgtccg tgaagtccgc ttgatcgacg acgaagaggg acaggttgga attgtcccgg
     1381 tgcagcaggc gctgcagatg gcgcgcgaca agggactcga tcttgtggag gtagcgccca
     1441 acgctgttcc gccggtctgt cgcattctgg actacggcaa gttcaaatac gagcagtcga
     1501 agaaagagcg tgaggcacgt aagcatcagc acaacgtcgt cctgcgcgag attcggatga
     1561 agccgaagat cgatgaccac gatgtcgact tcaagacccg gaccgtccag aagttgctca
     1621 aagagggtga caaggtcaag gtgagcgtca tgttccgtgg gcgtgaggtc acgcatccgc
     1681 agatcggacg tgccctcttg gaccgaatct acgagcgggt caaggaacac gggacgttgg
     1741 aaaaacagcc ttccatggaa ggcagattca tgactatgat cctggcgccg ggtcagccga
     1801 aggcggccaa ggacgcacca aagccaagga gtcaggcaca gccggaccag gagttggtga
     1861 cggcgccggt tggggagcaa taagttcgtg ccaaagatga agaccagcag cgccgccaag
     1921 cgccggttca gcgtgaccgg aaccggcaag gtgatgcgca tgaagggcaa taagcgacat
     1981 aagaagatgg cgaagtcccg tcgcgtgctc cagatggacg agcacaagtt tccggtggcg
     2041 tcgacgcatc gccggatcct ccagcgcctt ctgccctacg gagcctgatc tcgagatgtt
     2101 aggtattagg gaaacgaact gcctctaaac ctaacacctg acaaagtgag taacattcca
     2161 tgccacgagt gaaacgcggt gtcaccggac acgcgcgcca caagaagatt atcgagatgg
     2221 cgaagggcca caaaggtcag cgccatcggc tgttccgtcg ggcgaatgag tccgtcactc
     2281 acgcgctgtc gtacgcctat cgcgaccggc gcgaccgcaa gagccagttt cgccggctct
     2341 ggatcctgcg aatcaacgcc gccgctcgac agcatggcct cagctacagc cggttgatcg
     2401 acggattggc gaaggccggc gttgtgctcg accgtaaggt gctggcggat attgccgtcc
     2461 gcgaccaagc ggcgttcggc aagttggtcg agcaggccaa ggcagggacc gcgtagaccc
     2521 taccctgagc gaacccacgg gccgcgtgcg ctcacgccgg cccaatgtcg atcaccggcg
     2581 cgaaccgccg tcgccgtcac gaggaggcgg ccggacgtga acgagggctg gctgaccgcc
     2641 gcgaagctcc tcgccgtggt ggcgttggtg cttgccaacg gcttctttgt cgcatcggag
     2701 ttcgcgctgg ttgccatccg tcgaagtcgt gtcgacgaga tggtttccca gggtgtgttg
     2761 ggcgccaagg gagtccaacg agcaacgcgc aacctcgacc acttcatcgc cgcaacgcag
     2821 ctcggcatca ccctcgccag cctcgccctc ggatggttgg gcgagcccac gctcgggcgc
     2881 ctgctcgaac accggatcgg ccacgtcccg ggtgggacca cgctcgcggt gatcatcgcc
     2941 ttctcgatta tcacgacctt gcacatcgtc gtcggtgagc ttgcgcccaa gtcgatcgcg
     3001 ctgcaatacc cggagcggac gtcgctggtc atcgctcaac cgctcggcat ctttgacttt
     3061 gtctttcgac cgttcatcat cgcactcaac agcatcggcc ggttcaccgt ccggatgatc
     3121 ggccttcaac cgccctcggg gcacgagttg gttcattctt cggaagagtt gaggattctg
     3181 gtcgaagcct ccggtcgagc cggagtgctg gaagagtccg agcgcgagat catcaaccgc
     3241 gcgttcgact tcgccgactt caccgcgcgc gagttgatga tgccgcgcac ggagttcgtt
     3301 gccgttccgg ctgacctatc cggacgggac ctgcttcaca cggtcgtcga gaaggggttt
     3361 agccgctacc cggtctacca gggctcttta gacgatatcc tcggggtcgt ccacgtcaag
     3421 gatctggtcg cggcgatgga ccgcgatagc ttggaaaaca tcaccgccaa ggatctggcc
     3481 cgaccagcga tgattgtgcc ggacacgctt ccggtcgatg atctcctcga ccgtttgcgg
     3541 gataccaacg cccggatggc actggtgatc gacgagtttg gcggcgtcgc aggcatcgtc
     3601 acaatggaga acgtgctcga acgattggtc ggccagttgc gcgatgagtt cgagccgcaa
     3661 agtcagaccg aggtggttca ccatccagac ggcagtgttt cgatcagcgg cttgacgctg
     3721 atctcggacg cgaatgaact gctgggactg gaattggatg acgccgaata cgacacggtc
     3781 ggtgggtttc tcttcggcca catcggccat ctgccccagg tgggcgactc agtcgaggtc
     3841 gatggtcacg ccctgcgtgt cgagtcgatg gatgaccggc gcgtcgatcg agtgctggtt
     3901 acttcaaacg gaccaaacgc gccggcggtc acgctccggg gccggaacgg cctcgaacaa
     3961 cccggcgatt cctaaaccac caaggtacga agacaccaag attcaagata acttggcgtc
     4021 ttcgtgcctt gtatgtcggt atgtcaatac ggtgtagcca gtcgcgcgct cgacggcgaa
     4081 ggctaggcgg aggcggggca ggtcgattgt gcctgggacc gacgagtccg acgccaagcg
     4141 ggacc
//
LOCUS       DASUGJ010000431         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_484556, whole genome shotgun sequence.
ACCESSION   DASUGJ010000431 DASUGJ010000000
VERSION     DASUGJ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484556"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /gene="rpsM"
                     /locus_tag="VFV93_09540"
     CDS             complement(<1..166)
                     /gene="rpsM"
                     /locus_tag="VFV93_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX5165625.1"
                     /translation="MARIAGVDIPREKRLEISLTYIFGIGRTRSQQICTALDIDPDTR
                     VRDLTDDEVAR"
     gene            complement(188..301)
                     /gene="rpmJ"
                     /locus_tag="VFV93_09545"
     CDS             complement(188..301)
                     /gene="rpmJ"
                     /locus_tag="VFV93_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX5165626.1"
                     /translation="MKVRPSVKKMCEKCRIIRRHGRVQVICENPRHKQRQG"
     gene            complement(312..536)
                     /gene="infA"
                     /locus_tag="VFV93_09550"
     CDS             complement(312..536)
                     /gene="infA"
                     /locus_tag="VFV93_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX5165627.1"
                     /translation="MPKPKEDAIVLEGTIVESLPNAMFRVELENGHKVLAHISGKMRM
                     HYIRILPGDKVQVELTPYDLTRGRITYRYK"
     gene            complement(700..1359)
                     /locus_tag="VFV93_09555"
     CDS             complement(700..1359)
                     /locus_tag="VFV93_09555"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX5165628.1"
                     /translation="MIPGARLIILGRQGAGKGTQCVRLSRHYVVPHISTGDMLRAAAR
                     EGSELGLMAKQIMEEGGLVGDEVMIGLVDERLRKPDADSRGYILDGFPRTVAQAQALQ
                     RITDDRPIHVVIDLDVPREIVLERLSSRRVCRDCGTNYTAPIAEARRMWICDVCGGDV
                     EHRADDTPDAVNRRLDLYETQTAPLINHYSKMGLLTVVNGVGHPDYVFKRVCDAVDER
                     R"
     gene            complement(1371..>2042)
                     /locus_tag="VFV93_09560"
     CDS             complement(1371..>2042)
                     /locus_tag="VFV93_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HEX5165629.1"
                     /translation="IALTLGIIVAVVFVELGQRRIPVQFAKRVVGRRMMGGQNTYIPL
                     KVNQSGVIPIIFASSILLLPAILASFMGNADPNSDNWLERARAELQQFVDQYILNSQN
                     AVYITLFAVLIIAFAYFYNSIAFDPVRQADQIRRQGGFIPGVRPGAQTESYLAKAVNR
                     ITLPGAVFIAIIAILPYLLLWAADVQQFPFAGTSVLIAVGVSLELMRQIDSQLMLRNY
                     EGFLK"
BASE COUNT          343 a          640 c          684 g          375 t
ORIGIN      
        1 tgcgggcgac ctcgtcatcg gtgaggtcgc gcacccgcgt gtcggggtcg atgtcgagtg
       61 cggtgcagat ctgctgggac cgggtgcgcc cgatgccgaa gatgtaggtg agcgagatct
      121 ccaatcgctt ctcgcggggg atgtcgacgc cggcgatacg tgccatgttc tcgtgacctg
      181 ttctttctca gccctggcgc tgcttgtggc gcgggttctc gcagatgacc tggacgcgac
      241 catgccggcg gatgattcgg cacttctcgc acatcttctt gacgctcggt cggaccttca
      301 cgactggact ctcacttgta tcgatacgtg atacggcctc gggtgaggtc gtagggggtg
      361 agctctacct gcaccttgtc gcccggcagg atgcggatgt agtgcatccg catcttgccc
      421 gagatgtggg ccaacacctt gtggccgttc tccagctcga cgcggaacat cgcgttcggc
      481 agcgactcga cgatcgtgcc ttcgagcacg atggcgtctt ctttgggctt cggcagaaat
      541 tgctcctaga tcattgagca gggccttcga ccctgacccg tggatgcgga caaccggtct
      601 gcgcacagac cggctgtcaa tgctatcgga ccagttcagg aacaccacaa gggtgcgccc
      661 atcgtccgcg ggattgtggt atgccgagcg cccggaccgt cagcgacgtt cgtcgacggc
      721 atcgcagacg cgcttgaaca cgtagtcggg atggccgaca ccgttgacca ccgtcaacaa
      781 gcccatcttg ctgtagtggt tgatcagcgg cgcggtctgc gtctcgtaga ggtcgagccg
      841 gcggttgacg gcgtccgggg tgtcgtcggc gcggtgttcg acgtcgccgc cgcacacgtc
      901 gcagatccac atcctgcgcg cctcggcgat cggcgccgtg taattcgtgc cgcagtcccg
      961 gcacacccgc cgcgaggaca gccgctcgag cacgatctca cgcggcacgt cgaggtcgat
     1021 cacgacgtgg atcggccggt cgtcggtgat ccgctgcagc gcttgcgcct gggcgaccgt
     1081 ccgtgggaag ccgtcgagga tgtacccgcg cgagtcggcg tctggcttgc gcagccgctc
     1141 gtcgacgagg ccgatcatca cctcgtcgcc gacgaggccg ccctcctcca tgatctgctt
     1201 cgccatcagg ccgagctcgg aaccctcacg ggcggcggcg cgcagcatgt caccagtgga
     1261 gatgtgcggc accacgtagt ggcgcgacag tcgcacgcac tgggttcctt tcccagcgcc
     1321 ctgacggccg aggatgatga gacgcgctcc cggaatcacc ggcgccccga ctacttcaag
     1381 aagccttcgt agttgcgcag catgagctgg ctgtcgatct gacgcatcag ctcgagcgag
     1441 acgcccaccg caatcagcac gctggtgccc gcgaacggga actgctgcac gtcggccgcc
     1501 cacaacagga gatacggcag gatcgcgatg atggcgatga acaccgcgcc cggcagcgtg
     1561 atgcggttga cggccttggc gaggtacgac tcggtctggg cgccgggacg cacgccgggg
     1621 atgaacccgc cctggcggcg gatctggtcg gcctggcgca ccgggtcgaa ggcgatcgag
     1681 ttgtagaagt aggcgaaggc gatgatcagc accgcgaaca gcgtgatgta gaccgcgttc
     1741 tgcgagttga ggatgtactg atcgacgaac tgctggagct cggcccgggc gcgctccaac
     1801 cagttgtcac tgttcgggtc ggcgttgccc atgaagctgg ccaggatcgc cggcagcagc
     1861 aggatggagc tggcgaagat gatcgggatg acgcccgact ggttgacctt gagcgggatg
     1921 tacgtgttct ggccgcccat catccggcgg ccgaccacgc gtttcgcgaa ctgaacaggt
     1981 atccgtcgtt gcccgagctc gacgaacacc acggcgacga tgatgcccag cgtcagggcg
     2041 at
//
LOCUS       DASUGJ010000432         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_900468, whole genome shotgun sequence.
ACCESSION   DASUGJ010000432 DASUGJ010000000
VERSION     DASUGJ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900468"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFV93_09565"
     CDS             <1..323
                     /locus_tag="VFV93_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase domain-containing protein"
                     /protein_id="HEX5165630.1"
                     /translation="VSARFYVPETDHTVASVFWEFMTSEGVIWESGAYVEEPLFESPF
                     YATGYPITEAYWSDVTLQGEVEAVLWQCFERRCLTYTPDNPDGWQVEAGNVGLHYYLW
                     RYGN"
     gene            635..>2528
                     /gene="glgB"
                     /locus_tag="VFV93_09570"
     CDS             635..>2528
                     /gene="glgB"
                     /locus_tag="VFV93_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX5165631.1"
                     /translation="MPSTEAAAEGIEAAVAPEANMEPEPPKRRAPRKKAADADQPAGE
                     TVAATPKKRAPRKTAKNGFEEPINDVASPIAGDLMLAQASEQTIPDALPMPATTAIPV
                     GAPVGAQDGVSLLTAGDAYLFNEGSHYRLYQKLGSHLMEGGTYFAVWAPNAERVSVIG
                     DFNGWDAGSHQMQASGDSGIWELFVPDVGQGTIYKYRIFSRHNGYVVDKADPFAVHAE
                     TPPKTGSIVWPLEYEWNDGDWMENRRWRNAPDAPISIYEVHLGSWMRVPEEDNRSLNY
                     REIAHRLAGHVSYLGFTHVEFLPVTEHPFYGSWGYQATGMFAPTSRYGTPQDFMYLVD
                     VLHQHGIGVILDWVPSHFATDEHGLTYFDGTHLFEHADPRQGFHPDWGSYIFNYGRNE
                     VRAFLLSSAMYWLDKYHIDGLRVDAVASMLYLDYSRKAGEWIPNQYGGRENLEAIEFL
                     RQFNTAVYGEYPDVQTIAEESTAWPMVSRPVYVGGLGFGMKWDMGWMHDTLQYFSKDP
                     IHRRYHQNYLTFRMLYAFNENFVLPLSHDEVVHLKGSLIGKMAGDDWQKFANLRALYG
                     YMYAQSGKKLLFMGAEIGQWSEWNHEGSLEWHLLQYDMHAGMQRWLVALNHLYRHEPA
                     MHELDFD"
BASE COUNT          499 a          782 c          825 g          422 t
ORIGIN      
        1 cggtatctgc cagattctac gtgcccgaaa ctgaccacac ggtcgcttcg gtgttttggg
       61 aattcatgac ctctgagggc gtcatctggg agagcggcgc gtacgttgag gagccgctat
      121 tcgagagccc cttctacgcc accggctacc caatcaccga agcctactgg tccgatgtga
      181 cgctccaggg cgaagtagag gccgtcctct ggcaatgctt cgagagacgc tgcctgactt
      241 acacgcccga caatcccgat ggctggcagg ttgaggcagg caatgtcggc ctgcactact
      301 acctgtggcg ctacgggaac tagtgcggct aactcctccc acgatgcacc ccctttccag
      361 tagcggaaag ggggtgcgct gtttggcaac catcttgcaa gttcatgggt tgcccactgc
      421 ttctcatcgt gcgttgggaa acatgctggg accgggcggc tgcggccagg tatccaacgc
      481 cgtgacacaa tcacgtccga cgacgcccga ttcgcgattc gctgggaggg gagacccctt
      541 gatcgacgag caacaacagg atccaacgat cccgacagat acagccgaga cggagcagcc
      601 gaagccggcc aggaagcgcg cgccacgaaa gacggtgcct tcgacggagg cggcggcaga
      661 ggggattgag gcggcggtcg cgccggaggc gaacatggag ccggagccgc cgaagcgtcg
      721 tgcaccacgc aagaaggcgg cggacgccga tcaaccggcc ggcgagacag tagcggcgac
      781 gccaaagaag cgtgccccac gcaagacagc caagaatggt ttcgaggagc cgatcaacga
      841 tgtggcctcg ccgatcgcgg gggatctgat gctggcccag gcgtctgagc agacgatccc
      901 cgacgcgctg ccgatgccgg cgacaacggc gatcccggtc ggggcaccgg ttggagcgca
      961 agatggtgtg tcacttctga cggccggaga tgcctacctg ttcaacgagg ggagccacta
     1021 ccggctctac cagaagctcg gctcgcacct gatggagggt gggacctact tcgcggtctg
     1081 ggcgccgaac gccgagcggg tgtcggtgat tggcgacttc aatggctggg acgccggcag
     1141 ccaccagatg caggcgtccg gcgattcagg catctgggag ctattcgtgc cggatgtcgg
     1201 gcaagggacg atctacaagt accgcatctt ctcgcgacat aacggatacg tcgttgacaa
     1261 agccgacccg ttcgctgtcc acgccgagac gccgccgaag accggctcga tcgtctggcc
     1321 gctggagtac gagtggaacg acggcgactg gatggagaac cggcgctggc ggaacgcgcc
     1381 ggacgcgccg atctcgatct acgaggtcca tctcggctcg tggatgcggg tgccggagga
     1441 ggacaaccgc tcgctgaact accgcgagat cgcgcaccgg ctggccgggc acgtctcgta
     1501 cctgggcttc acgcacgtcg agttcctgcc ggtgacggag caccccttct acggctcgtg
     1561 gggctatcag gcgaccggga tgttcgcgcc gaccagccgc tacgggacgc cacaggactt
     1621 catgtatctg gttgatgtcc tgcatcagca tgggatcggc gtcatcctcg actgggtgcc
     1681 gtcgcacttc gcgaccgacg agcacgggct gacctacttc gacgggacgc acctgttcga
     1741 gcatgccgac ccgcgacaag ggttccaccc ggactggggc agctacatct tcaactacgg
     1801 acggaatgag gtgcgggcgt tcctgctgtc gagcgcgatg tactggctcg acaagtacca
     1861 catcgacggg ctgcgggtcg atgcggtcgc ctcgatgctg tatctcgact actcgcgcaa
     1921 ggccggcgag tggatcccga accagtacgg cggacgggag aacctggagg cgatcgagtt
     1981 cctgcggcag ttcaacacag ccgtctacgg agaatacccc gacgtccaga cgatcgccga
     2041 ggagtcaacg gcctggccga tggtctcacg gccggtctac gtcggcgggc tgggcttcgg
     2101 gatgaagtgg gacatgggct ggatgcacga cacgctccag tacttctcga aagacccgat
     2161 ccaccgccgc tatcatcaga actacctgac gttccggatg ctgtacgcgt tcaacgagaa
     2221 cttcgtcctg ccgctctcac acgacgaggt cgtccacctc aaaggctcgc tgatcggcaa
     2281 gatggccggc gacgactggc agaagttcgc caacctgcgc gcgctctacg gttacatgta
     2341 cgcgcaatcg ggcaagaagc tgctcttcat gggcgccgag atcggccagt ggtcggagtg
     2401 gaaccacgag ggcagcctcg aatggcacct gctccagtac gacatgcacg ccgggatgca
     2461 gcgctggctg gtggcgctga accacctcta ccgacacgag ccggccatgc acgagctgga
     2521 tttcgacc
//
LOCUS       DASUGJ010000433         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_622053, whole genome shotgun sequence.
ACCESSION   DASUGJ010000433 DASUGJ010000000
VERSION     DASUGJ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622053"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VFV93_09575"
     CDS             <1..1035
                     /locus_tag="VFV93_09575"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5165632.1"
                     /translation="NLTDMAEYAKSCGITLRPHFKTHKTGKVAERQKALGSTGITVAK
                     IGEAEALVDAGVYDDFFIANEIVGPIKHKRLLALMERAKVRVAVDTRDSARGLNDAMG
                     AAGRTLDVVIELNTGQNRAGVYPNEALGLAETIRNEMPNLRVVGLMTHEGHAGATLAP
                     DGIVSTAIAAGKSIIETAEQLREHGFDISVVSVGSTPAAKHTTTIEGITEMRPGTYVF
                     NDNSGFRFGTKPEDCALRILATVTSRPAPDRAILDCGSKTLTSDKSVGREGYGYIVEY
                     PDAEIISLSEEHGWLRLPESAQAMKVGDKVEVIPNHVCPAVNLQDEMYLIRNGEVAET
                     WPVIARGRVR"
     gene            1161..>2068
                     /locus_tag="VFV93_09580"
     CDS             1161..>2068
                     /locus_tag="VFV93_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX5165633.1"
                     /translation="MPTDITRFYTYDELTEILRTHERAHPTLATLESIGQSFEGRDIW
                     LLTVTNQETGAAGEKPAMYIDGNIHAGEVTGSNIVLHTIDLLLDGYGSDDEVTALLDN
                     FTFYLAPRVQPDGAELYLTSPLTLRSSVRPWPYEDEDDGLTPQDINEDGLILQMRVRD
                     PKGEWRVSEQDARLMVKRRPDELTGEFYRLYTEGLLNEYVRGPVKLARSKYGIDQNRN
                     WPANWSSLQRGGGPFPLSEPETRAVATFVESHRNIVVVQNYHTTGGVILRSPCAVGDS
                     TLPKGDAEVYKKMGEIGESIVGYPCAS"
BASE COUNT          397 a          695 c          679 g          297 t
ORIGIN      
        1 aacctgaccg atatggcgga gtacgccaaa tcgtgtggga tcacgctgcg gccccacttc
       61 aagacgcaca agaccggcaa ggttgccgag cggcagaagg cgctgggctc gaccgggatc
      121 accgtcgcca agatcggcga ggccgaggca ctggtcgatg ccggcgtcta cgacgacttc
      181 ttcatcgcca acgagatcgt cgggccgatc aagcacaagc ggctgctggc gctgatggag
      241 cgggcgaagg tgcgggttgc cgtcgatacg cgcgactccg cgcgcgggct gaatgacgcg
      301 atgggtgcgg ccggcaggac gctggacgtc gtcatcgaac tcaacaccgg ccagaaccgg
      361 gccggcgttt atcccaacga ggcgcttggc ctagccgaga cgatccgcaa cgagatgccg
      421 aacctgcgcg tcgtcgggct gatgacccac gagggccacg ccggggcgac gctggcgccg
      481 gacggtatcg tctcaacggc catcgcggcc gggaagtcga tcatcgagac ggctgagcag
      541 ctgcgcgagc acggcttcga catcagcgtc gtctcggtcg gctcgacgcc ggccgccaag
      601 cacacgacca caatcgaagg catcaccgag atgcgccccg gcacctacgt cttcaacgac
      661 aacagtggct ttcgcttcgg cacgaagccg gaggactgcg ccctgcggat tctggcgacc
      721 gtcaccagcc gcccagcccc cgaccgcgcc atcctcgact gcggctcgaa gacgctcacc
      781 agcgacaagt cggtcggtcg cgagggctac ggctacatcg tcgagtaccc cgacgccgag
      841 atcatctccc tctccgagga gcacggctgg ctgcggctgc ccgaatcggc ccaggccatg
      901 aaggtcggcg acaaggtcga ggtcatcccc aaccacgtct gcccggccgt caacctccag
      961 gacgagatgt acctgatccg caacggcgag gtcgccgaga cctggccggt cattgcgcgg
     1021 gggcgggtgc ggtagaacgg gaaccgggat tcgggattcg gggacgggat acacagcatg
     1081 ttcctcgtgg acatccgatg tatctcagcc ccgaatcccg aatcccggtt cccgatcaaa
     1141 cgaaaggact cacacactcg atgccaaccg acatcacgcg cttctacacg tacgatgagc
     1201 tgactgagat tctgcgcacg catgagcggg cgcacccgac gctggcgacg ctggagtcga
     1261 tcgggcagtc gttcgagggg cgcgacatct ggctgctaac ggtcaccaat caggagaccg
     1321 gcgcggctgg cgagaagccg gccatgtaca tcgacggcaa tatccacgcc ggcgaggtga
     1381 ccggcagcaa catcgtcctc cacacgatcg acctgctgct ggatggctac ggcagcgacg
     1441 acgaggtcac ggcgctgctg gacaacttca ccttctacct cgcgccgcgg gtccagccgg
     1501 acggcgcgga gctgtacctg acctcgccgc tgacgctgcg ctcttccgtc cggccctggc
     1561 cgtacgagga cgaggacgac gggctgacgc cgcaggacat caacgaggac ggcctgatcc
     1621 tccagatgcg ggtcagggac ccgaagggtg agtggcgcgt ctccgagcag gacgcgcggc
     1681 tgatggtcaa gcgccggccg gatgagctga ccggcgagtt ctaccggctc tacaccgaag
     1741 ggttgctcaa cgagtacgtg cgcgggccgg tcaagctggc gcgctcgaag tacggcatcg
     1801 accagaaccg caactggccg gccaactgga gttcgctcca gcgcggcggc gggccgttcc
     1861 cgctctccga gccggagaca cgcgccgtcg cgaccttcgt cgagtcgcac cgcaacatcg
     1921 tcgtcgtcca gaactaccac acaaccggcg gcgtcatcct gcgctcgccc tgcgccgttg
     1981 gcgacagcac gctgccgaag ggggacgccg aggtctacaa gaagatgggc gagatcggcg
     2041 agagcatcgt cggctacccc tgcgcctc
//
LOCUS       DASUGJ010000434         4938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1455345, whole genome shotgun sequence.
ACCESSION   DASUGJ010000434 DASUGJ010000000
VERSION     DASUGJ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455345"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFV93_09585"
     CDS             complement(<1..425)
                     /locus_tag="VFV93_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5165634.1"
                     /translation="MGKRTITLTVNGQQHTSEVEDRLLLVHHIRENLGLTGTHIGCDT
                     TGCGACTVHWNGKPVKSCTVFAVQADGGEIATVEGLETAEGLHPLQEGFWQEHGLQCG
                     FCTPGMLMTTSALLADNPTPSEEEIREAISGNLCRCTGYV"
     gene            complement(519..1391)
                     /locus_tag="VFV93_09590"
     CDS             complement(519..1391)
                     /locus_tag="VFV93_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5165635.1"
                     /translation="MIPGQFDYHAAATLQEAIALLQQHGDDAKVMAGGQSLIPLMKLR
                     LAQPAHIIDINRVGGLSDISDDGGWLTIGALTRESAIEASEAVRAKYPMLLDTAKVIA
                     DPLVRNLATVGGNIVHADPANDHPATMLAYRAEAVAHGPNGDRTIPIDDFFVDAFETA
                     LAPDEILTAIRIPAPAALTGGAYVKFERKVGDYAIAAAAAQLSLDPDGTISQAGLALT
                     NVAFAPARSNRAEEALRGKPANDDTFQQAATIAAEDCDPAADLRGSVDYKRAMAKTMA
                     LRALRLAALRARDA"
     gene            1697..4282
                     /gene="glgP"
                     /locus_tag="VFV93_09595"
     CDS             1697..4282
                     /gene="glgP"
                     /locus_tag="VFV93_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX5165636.1"
                     /translation="MKPIRTFSVIPSLPPQLERLGDIARNLRWAWNHDAVALFRRLDE
                     SLWLESGHNPVLMLGRIDQTRLEEAAADEAFLVQLERVARDLDGYLARRDTWFSRLAG
                     PVESPRIGYFSMEFGLTDCVNIFAGGLGLLAGDHLKSSSDLGVPLVGVGLLYQQGYFR
                     QTLNDAGWQQEFAEENDFHNLPLSLERLADGTPLIVEAPHPGRTVKAQVWKASVGRVS
                     LYLLDTNIDSNRPEDRDITDQLYGGDLEMRIRQEIVLGIGGCRALRALGLAPVVYHMN
                     EGHSAFLSLERVCQLMQDDGLTFAEAREAASAGLVFTTHTPVIAGHDSFPPQLIDRYL
                     GEYIRGLGISRQEMLALGRKNPADEGEPFWMTVLALRMAAYSNGVSKLHGEVSRQMWQ
                     GLWPGLPEDEVPIGHVTNGVHLRSWVSDEMEAHYDRYIGSRWRNEPSTTPSWEQAAKI
                     PAEELWRTHERRRERLIVFARRRLQAQLEHRGVSQAELDRAGSVLDTEVLTIGFARRF
                     ATYKRATLILRDPERLVRLITDPERPVQFIFAGKAHPRDDAGKELIRQIIAFSRQPAI
                     RNRFIFLEDYDMDTARFLVQGCDVWLNNPRRPEEASGTSGMKAAANGVLNFSTLDGWW
                     AEAWDDCADEPVPVGWAIGRGETYANWDYQDQVEAEDIYDVLERDIIPSFYERGAERL
                     PRGWIARMQSTIEHLGPFVGAHRMLRDYVTDFYLPAIERSSALAADDLAGARSLADWR
                     KRVEQHWSDVRVEAIQNNVPREVRVGEPMVAKAEVHLGALKPEDVAVQMYTGRLNATG
                     EIVDALPTPMHLAGETSDGTYQFQSDPTPWRVSGLHGYTVRVLPAHVALHCRYLPGLI
                     TWAKP"
     gene            complement(4300..4854)
                     /locus_tag="VFV93_09600"
     CDS             complement(4300..4854)
                     /locus_tag="VFV93_09600"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5165637.1"
                     /translation="MRIEARMEELGLVIPEPLKLPPGVELPFPWVRVHGNHAYISGHG
                     PQLADGTLAGPFGKVGADVSAEEAYEAARLTGLGIIGSLKRALGDLDRVTAWLSAFGM
                     VNAGPGFTSTPGVINGFSDLILAIWGPDAGSHARSAVGMAELPFGIPVEIEATVEVTG
                     DRCQEIGEINKSVLPQTCQLTPDT"
BASE COUNT          775 a         1640 c         1659 g          864 t
ORIGIN      
        1 acataaccgg tgcaacggca gaggttgccg gagatcgcct cgcggatctc ctcctcgctc
       61 ggcgttgggt tgtcggccag cagcgccgat gtcgtcatca gcatccctgg cgtgcagaag
      121 ccgcactgga gcccgtgctc ctgccagaac ccctcctgga gcgggtggag tccctcagcc
      181 gtctccagcc cctcgacggt cgcaatctcg ccgccgtcgg cctggacggc gaagaccgtg
      241 cagctcttga ccggcttgcc gttccagtgg accgtgcagg cgccgcagcc ggttgtgtcg
      301 cagccgatat gggtcccggt cagcccgagg ttctcgcgaa tgtgatgcac cagcagcagc
      361 cggtcctcaa cctcgctggt atgttgctgg ccgttgaccg tgagtgtgat ggttcgtttg
      421 cccatggtgt tccctttcag atcgggattc gggaaccggg attcgggagt gcttgaggta
      481 atgagatcgt tcgatatccc cgagtcccga gtcccgactt acgcatccct cgcccgcagc
      541 gcggccagcc gcaacgcccg tagcgccatc gtcttcgcca tcgcccgctt gtagtcgacg
      601 ctaccgcgca ggtcggcggc ggggtcgcag tcctcggcgg cgatcgtggc cgcctgctgg
      661 aaggtgtcgt cgttggccgg tttgcctcgt aacgcctctt cggcgcggtt cgagcgggcc
      721 ggggcgaagg ccacgttggt cagcgcgagg ccggcctgag agatcgtgcc gtcggggtcg
      781 agcgagagct gggcggcggc agcggcaatc gcgtagtcgc cgaccttgcg ctcgaacttg
      841 acgtaggcgc cgcccgtcag cgccgccggc gccgggatgc ggatcgccgt cagaatctcg
      901 tcgggcgcga gtgccgtctc gaaggcgtcg acgaagaagt cgtcgatcgg gatcgttcgg
      961 tcgccgttcg gaccgtgggc gacggcctcg gcgcggtagg cgagcatcgt cgccgggtgg
     1021 tcgttggccg ggtcggcgtg gacgatgttg ccgccgacgg tcgccaggtt gcggacgagt
     1081 gggtcggcga tgaccttggc tgtgtccagc agcatcgggt acttcgcgcg gaccgcctcg
     1141 gatgcctcga ttgccgactc gcgggtcaac gcgccgatcg tcagccagcc gccgtcgtcg
     1201 ctgatgtcgc tgagcccacc gacgcggttg atgtcgatga tgtgggcagg ctgggcgagt
     1261 cgcagcttca tcagcgggat caggctctgg ccgccggcca tcaccttcgc gtcatcgccg
     1321 tgttgctgca ataacgcgat cgcctcctga agcgtcgccg ccgcgtgata gtcgaactgt
     1381 ccgggtatca ctgattcgcc tccctccggt cgaccgtggc cgggtagcac gacaggcgct
     1441 gccgccgggc ggcaacagcg atgggatgta cctggcctga gctgcgctcc ctcttgggta
     1501 tgttacgacg ctgcgggcag tataggttcg gtttctgaac tcgtcaaaag cgcgaaagct
     1561 ctgtcatggc gcgattggcg tattcggcca ctcggcggca ggactgaagg gggcattggg
     1621 cagcaggggt attcttcatg cgacgacgcc gcttgtgtcg tgagtttcgg tagtcatgca
     1681 ctggacgatg ctacagatga agccaattcg aaccttctcc gtcataccgt cgcttccgcc
     1741 gcaactggag cggctcggtg atatcgctcg taacctgcgc tgggcctgga accatgacgc
     1801 ggtcgccctc ttccggcggc tggacgaaag cctctggctg gaatcgggtc acaacccggt
     1861 cctgatgctg gggcggatcg atcagacccg gctggaagag gccgccgccg acgaggcgtt
     1921 cctggtccag ctcgaacggg tcgcccgcga tttggacggc tacctggccc gccgcgacac
     1981 ctggttcagc cggctggccg ggccggtcga gagcccacgg atcggctact tctcgatgga
     2041 gttcggcctg acggattgcg ttaacatctt cgccggcggt ctcgggctgc tggccggcga
     2101 ccacctcaag tcgtcgtcgg acctcggcgt cccgctcgtc ggcgtcggcc tgctctatca
     2161 gcagggctac ttccgccaga cgctcaacga cgctggctgg cagcaggagt tcgccgagga
     2221 gaacgacttc cacaacctgc cgctcagcct cgaacggctg gcggatggca cgccgctgat
     2281 cgtcgaagcg ccgcatccgg ggcggaccgt caaggctcag gtctggaagg cttccgtcgg
     2341 ccgtgtctcg ctctacctgt tggacaccaa catcgacagc aaccggccgg aggaccgcga
     2401 catcaccgac cagctctacg gcggcgatct ggagatgcgt atccgccagg aaatcgtgct
     2461 cgggatcggc ggttgccggg cgttgcgggc gctcgggctg gcgccggttg tctaccatat
     2521 gaatgaaggg cactcggcct tcctgtcgct ggagcgggtc tgccagctca tgcaggatga
     2581 cgggctgacg ttcgccgagg cgcgcgaggc agcttcggcc gggctggtct tcacgactca
     2641 cacgccggtg atcgccggtc acgactcctt cccgccgcaa ctgatcgacc gctacttggg
     2701 agagtacatt cgcgggctgg gcatcagccg gcaggagatg ctggcgctgg gccggaagaa
     2761 cccggccgac gagggcgagc cattctggat gacggtgctg gcgctacgga tggccgccta
     2821 ttccaacggc gtctcgaagc tgcacggcga ggtcagccgc cagatgtggc aggggctctg
     2881 gccgggcctg ccggaagacg aggttccgat cggccacgtc accaacggcg tccacctgcg
     2941 ctcgtgggtt tcggacgaga tggaggcgca ctacgaccgc tacatcggct cgcgctggcg
     3001 caacgagccg tcgaccactc ccagttggga gcaggcggcc aagatcccgg ccgaggagct
     3061 ctggcgcacg cacgagcggc gccgcgagcg cctgatcgtc ttcgcccggc gacgcttgca
     3121 ggcgcaactg gaacatcggg gggtctcgca ggccgagctc gaccgggccg gttccgttct
     3181 cgacaccgag gtgctgacga tcggcttcgc ccggcgcttc gcgacctaca agcgcgcgac
     3241 gctcatcctg cgcgatccgg agcggctggt acgcctgatc accgaccccg agcggccggt
     3301 ccagttcatc ttcgccggta aggcccaccc gcgcgacgat gccggcaagg agctgatccg
     3361 tcagatcatc gccttctcgc ggcagccggc gatccgcaat cgcttcatct tccttgaaga
     3421 ctatgacatg gacacggcac gcttcctcgt ccagggctgc gatgtctggc tcaacaaccc
     3481 acgacgaccg gaggaagcct ccggcacgag cggcatgaag gcggcagcca acggtgtcct
     3541 caacttcagc acgctcgatg gctggtgggc cgaagcctgg gacgactgtg ccgacgagcc
     3601 ggtcccggtc ggctgggcga tcggccgggg cgagacctac gccaactggg actaccagga
     3661 ccaggtcgag gccgaggaca tctacgacgt gctggagcgt gacatcatcc ccagcttcta
     3721 cgagcgcggc gcggaacggc tcccgcgtgg ctggatcgcg cgcatgcaat cgacgatcga
     3781 gcacctcggg ccgttcgtcg gcgcccaccg gatgctgcgc gactacgtga ccgacttcta
     3841 cctgccggcc atcgagcgct catcggcact ggcggcggac gatctggccg gcgcgcgctc
     3901 gctggcggac tggcgcaagc gcgtcgagca gcattggtca gacgtgcgcg ttgaagcaat
     3961 ccagaacaac gtgccccgcg aggtgcgcgt cggcgagccg atggtggcga aggccgaggt
     4021 ccacctcggc gcgctgaagc cggaggatgt cgctgtccag atgtacaccg gccgcctcaa
     4081 cgcgaccggc gagatcgttg acgccctgcc gacaccaatg cacctggccg gcgagacgag
     4141 cgacggcacc taccagttcc agtcggaccc gaccccctgg cgcgtcagcg gcctgcacgg
     4201 ctacaccgtc cgcgtcctcc ccgcccacgt cgccctccac tgccgctacc tgccagggtt
     4261 gatcacgtgg gcgaaaccat aggtatcagg tgacaggtat caggtgtcag gagttagttg
     4321 gcaggtttgt ggcagcacgc tcttgttgat ctcaccaatc tcctgacacc tgtcacctgt
     4381 cacctccacc gttgcctcaa tctcaaccgg aatcccgaat ggcagctcgg ccatgccgac
     4441 ggcggagcgg gcgtggctgc cggcgtcggg tccccagatg gcgagaataa ggtcggagaa
     4501 gccgttgatg acacctggcg tgctggtgaa gccggggccg gcgttgacca tcccgaaggc
     4561 gctcagccag gccgtcaccc ggtcgaggtc gccgagggca cgcttgaggc tgccgatgat
     4621 gcccagcccg gtcagccggg ccgcctcgta ggcttcctcg gcgctgacat cggcgccgac
     4681 cttgccgaac ggccccgcca gcgtgccgtc cgctagttgc ggtccatggc cggagatgta
     4741 ggcgtggttc ccgtggacac gcacccacgg aaacggcagc tcaacccccg gcggcagctt
     4801 cagcggctcc gggatgacca gccccagctc ctccatccgc gcttcgattc gcaatggtgt
     4861 gtcctttcac tgtcagcgca cgttgcgtct cagacgaacg gtgctcacga ggacccggcc
     4921 cccttgccct gtcatcct
//
LOCUS       DASUGJ010000435         6378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1455968, whole genome shotgun sequence.
ACCESSION   DASUGJ010000435 DASUGJ010000000
VERSION     DASUGJ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455968"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..636
                     /locus_tag="VFV93_09605"
     CDS             346..636
                     /locus_tag="VFV93_09605"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX5165638.1"
                     /translation="MQVASRAIHINPSIVSADLDNETVLLNVETGLYFGLDELGSLIW
                     SMLAEGESRETISERLLAIYDVEPVRLLHDVDAFLDLLEANGLAYVNDPGTH"
     gene            636..1118
                     /locus_tag="VFV93_09610"
     CDS             636..1118
                     /locus_tag="VFV93_09610"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEX5165639.1"
                     /translation="MVRTTEISTTLANLRLVARAWIDLARVDRQLRHGGLEPVIDRIE
                     RDAAAPLPTIAPQDIRRAFRYARRIEQASHRHPARPQCLHRSLVLQRWLRSEGFPSEL
                     KIGVRKDEQQLKAHAWVEIGGVTINDRPAAVNAFTPLRRQPELAPVPATGSDTIGGQP
                     "
     gene            1115..2122
                     /locus_tag="VFV93_09615"
     CDS             1115..2122
                     /locus_tag="VFV93_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165640.1"
                     /translation="MTVMTVDADRAPRTPAISMAPYTLYGLAVSSDLPLPVPLANGDL
                     ERAVDLSFRLAGDAADAPPTAGELIAWAPCAIHGADARVTRGDDGTWIWHRAIGTFLV
                     SADARQVAVYPNATADPRAVGLTLAGPVALYMLHRRGRPSLHATAVHYAGRTIAFLGE
                     QGHGKSTMAAGFIADGATLLTDDALPLEDAGDVIYGHPGLPFMKVWTATAECALGLTG
                     ELPDLMDNYAKKYLDTEGRMRFATSPLPLDAVLLLRRYDAVAAGRDDVAITRLDQADA
                     VMALLAQTSNRSYLLPREQAGFLAPYARLATSIPVAILSYPSGFEFLPDVRQRILEYL
                     A"
     gene            2119..4083
                     /locus_tag="VFV93_09620"
     CDS             2119..4083
                     /locus_tag="VFV93_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX5165641.1"
                     /translation="MSGIAAIFNRDGRPVQQDSLWQMLGPVLQRGLDGCRVATDGPTG
                     FGYAKLTLTPDERDERQPLVGPRTGCMIAADVRLDNRSELLALLGPGIAPGVSDATLI
                     LAGYERWGAGVFGRLLGDFAVVIWDPRSRRVLAARDPGALRPLYYHSRPDRILLASDI
                     AQIVVDPSVPIAPNEQRIIESLLPASIYKNETDHAETWLAGIQAVPAGHYLVVNTDAL
                     SIQRHWELQVRPIRYRTQAEYAEHFRSLMSDAVATRLRSVSPVGVMLSGGLDSATIAC
                     LAQEQYRQGRLPDTGFVAFHIGFDGLVCDEREYIADIQAKYGFDVRHVSPGQFGGRLQ
                     LAPASLQTTPNLGVQASRQALFEQIVAANARVVLTGDVADACVIGSAFVFDSLLRQGR
                     FREFARRFSAYHRASTDSRARILVLNCLAPLGPMALQKAIMQRYLQRNYERGRARLIP
                     LYIEHGLRETLTDRQIAIALEAEQSRRFASPARETEYRTLYPPQAATPNGGMPIQFAR
                     PFADTRLHAFLLGIPPEEKYSPHPTVPGWYASSKQIIRRSMVGILPDSVRTRTDKTIF
                     EGVVTNEFQQQWPDYVDAFGPASRSELAKRGLIDQAEFFKRLGEYRDGQFGGDFSYMT
                     HLVGLETWLRSLALPRSTLTALAYETAGIV"
     gene            4227..4409
                     /locus_tag="VFV93_09625"
     CDS             4227..4409
                     /locus_tag="VFV93_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165642.1"
                     /translation="MKKTYASPTLVEYGKAYDLTLGTTGLSPDLVIIDGQLVPNPDNP
                     NCDNNGNPYLACVPIP"
     gene            4510..6288
                     /locus_tag="VFV93_09630"
     CDS             4510..6288
                     /locus_tag="VFV93_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5165643.1"
                     /translation="MSVLRRSLLEFRPFWRAWIPLLVLAAATPAIVLAMPLVEKYLID
                     DVILEKRYDRLPMALALWGGLWLLSLVLQIAGRAIGTYLGERYTLHMRHLLFTQYQKL
                     SVAFGRREHTGRTASLFINDAPNLSSLFSSVIVGGAGIIITLILAIIVMFSLNWQLAM
                     VAGLLPVLLGGAATIVARPLRPASRNAQEKTAELNEEIHENLSGLREITLFGQSGAQQ
                     ARLDTTMRQLLRLRMRLVGIEAGLGASQSLVSLAMTMSILGFGGYLVIHDRTTLGTLV
                     AMRSLFGNIFQPASRIASVVSSVQRSLGSADRIFAFMDQTPEVEDRPNATFPSRVAGR
                     IQFESVSFAYDGTQVIHDLSFLAEPGELVALVGPSGAGKSTTAGLIARFYDPCDGRIL
                     LDGHDLRDLDLSRLRETIGMVFQDTYLFADTIRANIAFGRIGATEAEVIDAAITANAW
                     EFIERLPDGLESAVGERGVWLSEGQKQRIAIARALLRDPKILILDEPTSALDARSEHL
                     LQSALDNLMAGRTTFVIAHRLATVRRADRILVIDGGRIVEQGSHDDLLARHGLYRELF
                     DLQFRPTDLLPVASDPAPSPVALIGD"
BASE COUNT         1057 a         2253 c         1993 g         1075 t
ORIGIN      
        1 tcgtcaactc cacgatgcga ctgagcaggc aggcgcattg cgcgaggcgg agcacctgct
       61 ggtcgcttcg cgcgcgccgg ctcgccccgg agagcacggc gactccccgc gccccgacag
      121 gctggccgtc cagccgtagc gatggacaac gatcccctcg ccggcggtgt tgcacaccgc
      181 gcgacgccgg ctgcgggcgc tcaacaagac ctcaacacca actagactgg caagccacac
      241 cggatccgta atcttgccgt gtgagttccg ctttacgtcg aatgatttgc ctcagcaaat
      301 ggcgaccctc acgtgttcgc gccaatgttg tcgggagtta cactgatgca ggtagccagc
      361 cgcgccatcc acatcaatcc gtccatcgtc tccgcagatc tcgacaacga gacggtcctt
      421 ctcaacgttg aaaccggcct ctacttcggg ctggatgaac tgggctcact gatctggagc
      481 atgctggccg agggcgagtc gcgcgagacg atctccgaac gcctgctggc catctacgat
      541 gtcgagccgg tccgtctgct ccacgatgtc gacgcgttcc tcgatctgct ggaggcgaac
      601 ggcctcgcct acgtcaacga tcccggaaca cactgatggt gcgcacaacc gaaatcagca
      661 ccactctcgc caacctccgc ctcgttgcgc gggcctggat cgacctggcc cgggtcgacc
      721 ggcaactgcg tcacggcggc ctcgaaccgg ttatcgaccg tatcgaacga gacgcggctg
      781 cgcctctacc gacgattgcc ccgcaggaca tccgccgggc gtttcgctac gcgcgcagga
      841 tcgagcaggc gtcgcaccgg catccagccc gcccacaatg cctgcaccgc tcgctcgtgc
      901 tccagcgctg gctgcggtcc gaggggtttc cgagcgagtt gaagatcggc gttcgcaagg
      961 acgagcagca gctcaaagcc cacgcctggg tcgagatcgg cggggtcacc atcaacgacc
     1021 ggcccgccgc cgtcaacgcc ttcacacccc tgcgacgcca gcctgaactg gcgcccgtcc
     1081 cggcgaccgg cagcgacacg atcggtggac aaccatgacg gtgatgacgg tcgatgccga
     1141 ccgcgcccca cgcaccccag ccatttcaat ggctccgtat acgctgtatg gcctggcggt
     1201 ttccagtgat ctgccgctgc cggttcctct cgcgaacggc gacctggagc gggcggtcga
     1261 tctgagcttc cgtctggccg gtgatgccgc cgacgccccg ccgactgcgg gggaactgat
     1321 cgcctgggcc ccatgcgcca tccacggcgc cgatgcccgc gtcacccgtg gcgacgacgg
     1381 cacctggatc tggcaccgcg cgatcggcac cttcctggtg tcggccgacg cccgccaggt
     1441 tgccgtctat ccgaacgcga cagccgatcc ccgcgccgtc gggctgacgc tggccggccc
     1501 ggtcgcgctc tacatgctgc accgtcgcgg gcgtccaagc ctgcacgcca ccgccgtcca
     1561 ctacgcggga cggacgatcg cctttctcgg cgagcaggga cacggcaagt cgacgatggc
     1621 agccggcttc atcgccgatg gcgccacgct gctgaccgac gacgcgctgc cgctcgaaga
     1681 cgcaggcgac gtcatctacg gccatccggg actgccgttc atgaaagtct ggaccgcgac
     1741 cgccgagtgc gcgctggggc tgaccggcga actgcccgac ctgatggaca actacgcgaa
     1801 gaagtacctc gacaccgagg gccggatgcg cttcgcgacg tcgccgctgc cgctggatgc
     1861 cgtcctgctg ctgcgccgct acgacgcggt tgctgccggt cgggatgacg tcgcgatcac
     1921 gcggcttgac caggccgacg ccgtcatggc gctgctggcg cagacgtcca accgctccta
     1981 cctgctcccc cgcgagcagg ccggcttcct cgcgccgtac gcgcggctgg caaccagcat
     2041 cccggtcgca attctgagct acccatccgg gttcgagttc ctgccggacg ttcggcagcg
     2101 cattctggag tacctggcat gagcggaatc gccgccatct tcaaccgcga cggccggccg
     2161 gtccagcagg acagcctctg gcagatgctc gggccggtgt tgcagcgcgg gctggatggt
     2221 tgcagggtcg cgacggatgg cccaaccgga ttcggctacg ccaagctgac gctgacgccg
     2281 gatgaacgcg acgagcggca accgctggtt ggcccgcgaa ccggctgcat gatcgccgcc
     2341 gacgtgcggc tcgacaaccg gtcggagctg ctggccctgc ttgggccagg gatcgccccg
     2401 ggtgtctccg acgcgacgct gattctggcc ggctacgagc gctggggagc gggcgtcttc
     2461 gggcggctgc tgggcgactt cgcagtcgtg atctgggacc cgcgcagccg gcgcgttctc
     2521 gccgcccgcg atcccggcgc gctccgaccg ctctactacc acagccggcc cgaccgaatc
     2581 ctgctggcgt ccgacatcgc ccagatcgtc gtcgatccct cggtgccgat cgcgccgaac
     2641 gagcagcgca tcatcgagtc gttgctgccg gccagcatct acaagaacga gacggaccac
     2701 gccgaaacct ggctcgcagg tattcaggcc gtcccggccg gccactatct ggtggtgaac
     2761 acggatgccc tcagcatcca gcgccactgg gagctacagg ttcgcccaat ccgctaccga
     2821 acgcaggccg agtacgccga gcatttccgc agcctgatgt ccgacgcggt cgccacgcga
     2881 ctgcgaagcg tctcgccagt cggcgtcatg ctcagcggcg gtctggattc ggcgacgata
     2941 gcctgcctcg ctcaggaaca gtaccggcag gggcgactgc ccgacaccgg cttcgtcgcc
     3001 ttccacatcg gcttcgatgg gctggtctgt gacgagcgtg agtacatcgc tgatatccag
     3061 gcgaaatatg gatttgatgt ccgccatgtt tcaccgggtc agttcggcgg ccgcttgcag
     3121 cttgcgccgg cctcgttaca gacgactcca aatctcggtg tccaggcatc gaggcaggcg
     3181 ctgttcgagc agatcgtcgc cgccaacgcg cgcgtcgtcc tgaccggcga tgtcgccgac
     3241 gcctgcgtga tcggctccgc gttcgtcttc gactcgctgc tgcggcaggg ccgcttccgc
     3301 gagttcgcgc gtcggttcag tgcctaccat cgggcttcga ccgattcgcg agcgcgcatc
     3361 ctcgtcctca actgcctggc gccgctgggg ccgatggcgc tccagaaggc gatcatgcag
     3421 cgctacctgc aacgcaacta cgaacgtgga cgcgccaggc tgatcccgct ctacatcgag
     3481 cacggcctac gggagacgct gaccgaccgt cagatcgcaa tcgcactgga ggccgagcag
     3541 tcccggcgct tcgccagccc agcgcgggag acggagtacc gcacactgta cccgccacag
     3601 gccgcgacac cgaacggcgg gatgccgatc cagttcgcgc ggccatttgc ggacacgcgg
     3661 ctacatgcgt tcctgctcgg catcccgcct gaggagaagt actcgccgca cccgaccgtc
     3721 ccgggctggt acgcgtcatc gaagcagatc atccggcgct cgatggtcgg catcctgcct
     3781 gacagcgtcc gcacccgcac cgataaaacg atcttcgaag gtgtcgtcac gaacgagttc
     3841 cagcagcagt ggccggatta cgtcgatgcc ttcgggccgg ccagccgctc cgagttggcc
     3901 aagcgcgggt tgatcgatca ggcggagttc ttcaagcgac ttggcgaata ccgcgatggt
     3961 cagttcggcg gtgacttctc gtacatgacc catctcgttg gccttgagac ctggctgcga
     4021 tcgctggcgc tgccgcgatc aaccctgacc gcgctagcct acgagacggc tggcatcgta
     4081 tgagcaccaa cgtcccaccc tgctttcact cctcggcgcc gtcgcttccg cgctcgcacc
     4141 ggatgacggc gacgagacct cctgcatcgc ccctggcacg ggctgcatgt gtcgaacaat
     4201 ctgccgcgaa gggaggtgaa tggtcgatga agaagacgta cgcgagccca acgctcgtgg
     4261 agtacggcaa agcctacgat ctcacgctgg gaacaactgg gctctcgcca gatctggtca
     4321 tcattgatgg tcagctcgtc ccgaacccgg ataacccaaa ctgcgacaac aacggcaacc
     4381 cgtacctggc atgcgtgcca atcccgtaac cggtgacctg acgagtgcga gcgtcaagcc
     4441 tgatggcagg gccggtggca agccgccggc cctgctcctc attgcagctg attgtgagac
     4501 gagccaaccg tgagcgtcct gcgacgcagc ctactcgaat ttcgcccctt ctggcgcgcc
     4561 tggataccgc tgctggtgct ggcggcggcc acgccggcca tcgtcctggc gatgccgctc
     4621 gtcgaaaaat atttgattga tgatgtcatt ctcgaaaagc gttacgaccg cctgccgatg
     4681 gcgctggccc tctggggcgg gctctggctg ctgagcctgg tcctccagat cgccggccgg
     4741 gcgatcggca cctacctcgg cgaacgctac acgctccaca tgcgccacct gttgttcacc
     4801 cagtaccaga agctctcggt cgcgttcggc cgccgcgagc acaccggcag gacggcgtcg
     4861 ctgttcatca acgacgcgcc gaacctgtcg agcctgttca gttcggtgat cgtcggcggc
     4921 gccgggatca tcatcacact gatcctggcg atcatcgtca tgttcagcct caactggcag
     4981 ctggcgatgg tcgccggtct cctgccggtc ctgctcggcg gcgcggccac gatcgtggcc
     5041 cgcccgctgc gtcccgcctc gcgcaacgcg caggagaaga cggccgaact gaacgaggag
     5101 atccacgaga acctctccgg cctgcgcgag atcaccctct tcggccagag cggcgcccag
     5161 caggcccggc tcgacacgac gatgcgacaa ctgttgcggc tgcggatgcg gctggtcggg
     5221 atcgaagccg gcctcggcgc cagccagagc ctcgtctcgc tggcgatgac gatgtcgatc
     5281 ctcggcttcg gcggctatct ggtcatccac gaccggacga cgctcgggac gctggtcgcc
     5341 atgcgcagcc tgttcggcaa catctttcag ccggccagtc ggatcgccag cgtcgtcagc
     5401 agcgtccagc gctcgcttgg ctcggccgac cgcatcttcg cctttatgga tcaaacacct
     5461 gaggtcgagg accggccgaa cgcgacgttc ccgtctcgcg tcgccggtcg tatccagttc
     5521 gagtcggtca gcttcgcata cgacggcacg caggtcatcc acgacctcag cttcctggcc
     5581 gagcctggcg agctggtcgc gcttgtcggg ccaagcgggg cgggcaagag tacgacagcc
     5641 ggcctgatcg cccgcttcta cgacccctgc gacggccgga tcctgctcga cggccacgac
     5701 ctgcgtgacc tcgacctcag ccgcctgcgt gagacgatcg ggatggtctt ccaggacacg
     5761 tacctgttcg ccgacacgat tcgcgccaac atcgccttcg gccggattgg cgcaaccgaa
     5821 gcagaagtca tcgacgcggc tatcacggcc aacgcctggg agtttatcga gcggctgccg
     5881 gacgggctgg agtcagccgt cggcgagcgc ggcgtctggc tctcggaggg gcagaagcaa
     5941 cgcatcgcga tcgcccgggc gctgctgcgc gacccgaaga tcctgatcct cgacgagccg
     6001 acctcggccc tcgacgcacg ctcggagcac ctgttgcaat ccgcgctcga caacctgatg
     6061 gcgggcagga cgacgttcgt catcgcccac cggctggcga ctgtccgccg tgccgaccgg
     6121 attctcgtca tcgacggcgg ccggatcgtc gagcagggca gccacgacga tctgctcgcc
     6181 cgccacggcc tctaccgcga gctgttcgac ctccagttcc gcccgaccga cctgctaccg
     6241 gttgcctcgg acccggcacc gtcaccagtc gccctgatcg gggattgagc gtgtcccctg
     6301 tcgtcctgag ccgcagcgaa ggacctctcc gcgtgataac cgtgtggcgt taggcggaga
     6361 ggtccttcgg ctgcggcc
//
LOCUS       DASUGJ010000436         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1597422, whole genome shotgun sequence.
ACCESSION   DASUGJ010000436 DASUGJ010000000
VERSION     DASUGJ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597422"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VFV93_09635"
     CDS             complement(<1..1245)
                     /locus_tag="VFV93_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5165644.1"
                     /translation="MRVQILGGGPGGLYAAILLKKSHPGWEIRLTERNPRDATYGWGV
                     VFSDRTLSGLHEADIRTSTQITEGFVLWDAIDIHFRDELIRCDGHAFAGIGRKTLLNI
                     LQARCDELGVEMHFETEVDDPAALRADCDLLIAADGVNSRTRMAFAESFQPTTSEGRA
                     RFVWYGTDKPFDAFTFIFRETEYGLFTVHSYPFDGTTSTFIVECHEETWRKAGLDRMD
                     EAESMAFCQLLFADHLGPHRLLSNRSLWLAFTTLKCRRWAHDNVVLLGDAAHTAHFSI
                     GSGTKLALEDAIALAQAFEQHDRLQPALRSYEQARRPRVEATQRAAAESQRYFESISR
                     YRHFAPRQFAFHLLTRSGRITWDNLRGRDPYFVAGVERWFQATARRDDDATTLVTPPP
                     MFAPLRLRDLTLTNRVVMSPDPS"
     gene            complement(1242..2102)
                     /locus_tag="VFV93_09640"
     CDS             complement(1242..2102)
                     /locus_tag="VFV93_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5165645.1"
                     /translation="MELGIGLPNTVKGVSGQTTLEWARRADEGPFASVGVFDRLVYDS
                     LDPLVTLAAVAGATRRVRLATTVVIGPLRNPVGLAKEAASLDVLSGGRLTLGLALGAR
                     RDDYEAAGVPYAERGRRLDAQLRILRDTWEDGRIGPTPLQPNGPELIVGGLDDAAFAR
                     AARYADGFVHNGGPPRIFARSVDKALAAWQDAGRPGRPRIWGMAYFALGDDEARAGHD
                     YLLDYYAFTGPFAERIAAGLLTTPQAIIQHIRGYAEAGCDHLVLFPTSSAVDQLERLA
                     QVVGTHESST"
     gene            complement(2120..>2592)
                     /locus_tag="VFV93_09645"
     CDS             complement(2120..>2592)
                     /locus_tag="VFV93_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-aminobenzoate-CoA ligase"
                     /protein_id="HEX5165646.1"
                     /translation="GTVGRLAVRGVTGCRYLADPERQRHYVQNGWNMTGDAYLMDADG
                     YFWYQARTDDMIISSGYNIAGPEVESVLLEHPLVRECAVIGTPDPRRTNLVTAIIVLN
                     DPTAASDELVTELQDYVKAQIAPYKYPRRVEFVESLPRTETGKLQRFKLRDERS"
BASE COUNT          437 a          857 c          878 g          420 t
ORIGIN      
        1 cgatggatca ggcgacataa cgacgcgatt cgtcagcgtc aggtcgcgta gccggagtgg
       61 ggcaaacatc ggcggtggtg tgaccagcgt cgtcgcatca tcatcgcgcc gcgcggtcgc
      121 ctggaaccag cgttcgacgc cggcgacgaa gtagggatcg cggccacgca ggttgtccca
      181 ggtgatccgg cccgagcggg tcagcaggtg gaaggcgaac tgacgcggcg cgaagtggcg
      241 gtagcgactg atgctctcga agtagcgctg gctctcggcg gcggcccgct gagtcgcctc
      301 aacgcgcggg cgacgtgcct gctcgtagga gcgcagggcg ggctggagtc ggtcatgttg
      361 ctcgaatgcc tgcgccagcg cgatcgcgtc ttcgagcgcc agcttcgtcc cggagccgat
      421 cgagaagtgg gccgtgtggg cagcgtcacc cagcaggacg acgttgtcgt gcgcccagcg
      481 gcggcacttc aacgtcgtaa acgcgagcca gagcgagcgg ttcgacagca gccggtgcgg
      541 gccgaggtga tcggcgaaca gcagttggca gaacgccatg ctctcggctt cgtccatccg
      601 gtccagcccc gccttacgcc acgtctcctc atggcactcg acgatgaagg tgctggtcgt
      661 cccgtcgaac gggtacgagt ggacggtgaa caggccgtat tccgtctcac ggaagatgaa
      721 ggtgaacgcg tcaaacggtt tgtcggtgcc gtaccagacg aagcgcgcac ggccctcact
      781 cgtggtcggc tggaacgact ccgcgaaggc catccgggtc cgtgagttga cgccgtcggc
      841 ggcgatcagc aggtcgcaat cagcccgtag ggctgcagga tcgtcaacct ccgtctcgaa
      901 gtgcatctcg acacccagct catcacagcg cgcctggagg atgttgagca gcgtcttccg
      961 tccaatcccg gcgaaagcgt gaccatcgca gcggatcaac tcgtcgcgga agtggatgtc
     1021 gatcgcgtcc cagaggacga acccctcggt gatctgggtc gacgtgcgga tgtcggcttc
     1081 gtgcagtccc gacagggtgc ggtcggagaa gacgacgccc cagccgtagg tggcgtcacg
     1141 gggattgcgc tccgtcagcc tgatctccca gccgggatgg gacttcttca gcaggatcgc
     1201 ggcatacagc ccaccagggc cgccgccgag aatctgcacc ctcatgtgga cgactcgtgg
     1261 gtgccaacca cctgcgccag gcgctccagc tggtcgacag cgctgctggt tggaaagagg
     1321 accagatggt cacaaccggc ctcggcgtag ccacgaatgt gctggatgat cgcctgcggt
     1381 gtcgtcagca gccctgccgc gatgcgttcg gcgaacgggc cggtgaacgc gtagtagtcg
     1441 agcaggtaat cgtggccggc acgggcctcg tcgtcaccga gcgcgaagta ggccatgccc
     1501 cagatgcgcg gtcgccccgg tcgcccggca tcctgccagg cggcaagcgc cttgtcgacg
     1561 gagcgggcga agatacgcgg cgggccgcca ttgtggacaa agccgtcggc atagcgcgct
     1621 gcccgggcga acgccgcgtc atccagcccc ccgacgatca gctccggccc attcggctgg
     1681 agcggcgtcg ggccgatccg gccgtcctcc caggtgtcgc gcaggatgcg tagctgcgca
     1741 tccagccgcc tgccgcgctc ggcatacggg acaccagccg cctcgtagtc gtcgcgcctg
     1801 gcgccgagcg ccagaccgag cgtcagccgc ccgcccgaga gaacatccag cgacgccgcc
     1861 tccttcgcca agccaaccgg attgcgtagc ggcccgatca cgaccgtcgt cgccagccgc
     1921 acccgccgag tcgcgccggc cacagccgcc agcgtcacca gcgggtcgag gctgtcgtac
     1981 accaaccggt cgaagacgcc gacgctcgcg aacggcccct cgtcggcccg tcgcgcccat
     2041 tcgagcgtcg tctggccgga gacacctttg accgtgttcg gaaggccaat ccccagttcc
     2101 atcgccgctc ctgctcccat cagctgcgtt cgtcgcgcag cttgaagcgt tgcagcttgc
     2161 cggtttcggt ccgtggcagg gattcgacga actcgaccct gcgcgggtac ttgtacgggg
     2221 cgatctgagc tttgacatag tcttgcagct cggtgaccag ttcgtcggag gcggcggttg
     2281 gatcgttgag gacgatgatg gcagtcacga ggttcgtgcg gcgcggatcg ggcgtgccga
     2341 tcacggcgca ctcccgcacc agcggatgct ccagcaggac gctctcaacc tcgggaccgg
     2401 caatgttgta gccggaggag atgatcatgt cgtcggtccg ggcctgatac cagaagtatc
     2461 catcggcatc catcaggtag gcgtcgccgg tcatgttcca gccgttctgg acgtagtgcc
     2521 gctgccgctc cggatcggcc aggtagcggc agccggtaac gccgcgtacc gccagccggc
     2581 cgaccgttcc cg
//
LOCUS       DASUGJ010000437         3604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1317964, whole genome shotgun sequence.
ACCESSION   DASUGJ010000437 DASUGJ010000000
VERSION     DASUGJ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317964"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..622)
                     /locus_tag="VFV93_09650"
     CDS             complement(113..622)
                     /locus_tag="VFV93_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5165647.1"
                     /translation="MAATKTSSDILAPENAKDRDKIVKLLTKAYWMEMETVMNYITNS
                     VNPDGVRAQEIIESLAQDVTEELGHAQQYAQRIKELYGVVPGSMDFKPEQTFLQPPEK
                     QTDIIHIIKGVIEAETGAIQHYSKIIEETDGIDYVTQDMVIAILHDEQGHRRLFEGFL
                     REFEADGLA"
     gene            787..>3604
                     /locus_tag="VFV93_09655"
     CDS             787..>3604
                     /locus_tag="VFV93_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HEX5165648.1"
                     /translation="MTQSQVFISYQRADEPFARQVREYLAANGVRTWMDQYDIPVGAY
                     WPDEIDKALAASDIVVGVLSPDSVESRNVKNEWDWAILNEKPLLMLQYRPCSIPHRYV
                     SINFIDASRPDTDAAFVALLDTIGGSSPAANGQTAPSPAAAGRARHGSRSGAPVRRRQ
                     EPFVVGREREQALLRARLDDLAAGRGSLILLAGEAGIGKTTLTNWLAWAAEQQGALVA
                     SGGCYDLTMTPPYGPWARMTGDWPDDPALPPMPSELREGTGMGAIRNQTEFIALFERF
                     LSDASVAWPLLLLLEDFHWADEASLDLLRSLARSISRMRVVLVVTYRDDELTRRHPLA
                     QMLPALAREDGAVRITLSRLALPATRNLVTGRYQFSKADTTRLGAYVHRLSEGNPFFC
                     GEILRELEEVGALSRAGDHWQITNLDRVRVPALVRQVIERRLERLDDDARSLLQIAAV
                     IGQEVPVDLWVEVSGASDEHLAETLERATEARLVVELRGGTAFSFTHALIRETLYESL
                     VSLRRRRWHRTTAEVLERMSRPDPDAIAHHYQQANDDRAFEWLIRAGRRAMERYSWRT
                     AVERWEAAAELLEGDDARRREFGWLLFLMGTHLRYASRERALDYLRDAQWIGHEIEDQ
                     LLRVCASAGIGFQRLFSGRIEGGLEDLVRSIADYDSLDPALIETVPDIVNRDSLLSLM
                     ENRRQMQVPGLVTFGHQLDALRLSDELLARPRTYPNNAAMTSMGNVYLGRGLALASMA
                     RPDEAADAFARAREEFETTSETNPYVFGIGAFFALHTVELPYHADQIERLEAVAHVGI
                     DAWVSARESATEGRSTRVAMLGMLLIHGDWDEAREIAEIGVAGEVIMVLWDEMVRVLG
                     LLNRHQGRPDQAWEQVARYFPDGPTASPSPTYVPTATALQRLAVALALDAGDVPLAHD
                     WLVAHDAWLDWSDSVAGHADGTLIW"
BASE COUNT          599 a         1109 c         1185 g          711 t
ORIGIN      
        1 ggtgcctgtt tggtcatggt gtgctgtttc tgttgaggga tgaggcatgg gggatggggg
       61 atgaggacgc atttcactca tcccccatcc cccatgcctc attcccgaaa ccctatgcca
      121 gcccgtccgc ctcgaactca cgcaggaagc cttcgaagag gcggcggtgg ccctgctcgt
      181 cgtgcaggat cgcgatcacc atgtcctggg tgacgtagtc gatcccgtcg gtctcctcga
      241 tgatcttgct gtagtgctga atcgcgccgg tctcggcctc gatcacaccc ttgatgatgt
      301 ggatgatgtc ggtctgcttc tccggtggct ggagaaatgt ctgctcgggc ttgaagtcca
      361 tcgaaccggg cacgacaccg tacagctcct tgatgcgctg ggcgtactgc tgggcgtggc
      421 cgagctcctc ggtcacgtcc tgcgccagcg actcgatgat ctcctgcgca cggacgccgt
      481 ccggattgac ggagttggtg atgtagttca tcaccgtctc catctccatc cagtacgcct
      541 tcgtcaggag cttgacgatc ttatcgcgat ccttggcatt ctcgggcgcg agaatgtcac
      601 tactcgtctt ggtcgcagcc atcgttggtt ccctccttag gggtcgtcca gctgtatcat
      661 ccattgatct gtcagcaggc aatttccgtg ccggtatctg acgcccggct cttgtttcca
      721 caacgggcat cggctatcgt cccggagcac agatcgccca tccgggaaca tagacgggtg
      781 tccgtcatga cgcagtcaca ggtcttcatc tcctaccagc gcgcggatga gccgttcgcc
      841 cgtcaggtcc gcgagtatct ggccgcaaac ggcgtccgga catggatgga ccagtacgac
      901 atcccggtcg gcgcctactg gccggacgag atcgacaaag cgttggcggc gtcagacatc
      961 gtcgtcggtg ttctgtcgcc ggattcggtc gagagccgca acgtcaagaa cgaatgggac
     1021 tgggcgatcc tgaacgagaa gccgctgctg atgttgcagt atcggccgtg ctcgatcccg
     1081 caccgctatg tctcgatcaa cttcatcgac gcgagccggc cggatactga tgcggcgttc
     1141 gttgcattgc tcgatacgat tggtggcagt tctccggcag cgaatgggca aactgctcca
     1201 tcccctgctg ctgcagggcg cgcacgacac ggttcgcgat cgggcgcacc ggttcgacgc
     1261 cggcaggaac cgttcgtcgt cggccgcgag cgggagcagg cgctccttcg cgcccggcta
     1321 gatgatctgg ccgccggtcg tgggagtctc attctgcttg ccggtgaagc cggcatcggc
     1381 aagacgacac tgaccaactg gctagcgtgg gccgctgaac agcagggcgc gctcgtcgcg
     1441 agcggtggct gctacgacct gacgatgacc cctccgtatg gcccgtgggc acgaatgacc
     1501 ggcgattggc ccgatgaccc ggccctgccg ccgatgccga gcgaactgcg cgaaggcacc
     1561 ggcatgggcg cgattcgcaa ccagaccgag ttcatcgcgc tcttcgaacg gtttctctcc
     1621 gatgccagcg tggcgtggcc actgttgctg ttgctggaag atttccattg ggctgacgag
     1681 gccagtctcg atttgcttcg gagtctggcg agatcgatca gcaggatgcg ggttgtcctc
     1741 gtcgtcacct atcgagacga cgagttgacg cggcgccatc cgctggccca gatgttgccg
     1801 gcgctcgccc gtgaggatgg ggccgtccgt atcaccctct cccggctggc gttaccggcc
     1861 acgcgcaacc tcgtgactgg ccgctaccag ttctcaaaag ccgatacgac gcggctcggc
     1921 gcatacgtcc atcgactctc agagggtaat ccgttcttct gcggagagat tctacgcgag
     1981 ctcgaagagg tcggggcgtt gtctcgcgct ggcgatcact ggcagatcac gaacctcgat
     2041 cgagtgcgtg tgcctgcgct cgtccggcag gtcatcgaac ggcgcctgga gcgactcgac
     2101 gacgatgctc gctccctgct gcagatcgct gccgtcatcg gccaggaggt gccggtcgac
     2161 ctctgggttg aagtcagtgg cgccagcgac gagcacctgg ctgagacgct tgaacgcgcg
     2221 accgaggcgc ggctggtcgt ggaactccgt ggagggacgg cgttcagctt cacccacgcg
     2281 ttgatccgcg agacgctcta cgagagtctc gtctcgctgc gccgccgtcg ctggcatcgg
     2341 acaacggccg aggtactcga gcggatgagc cggcccgatc cggacgcgat cgcccatcac
     2401 taccagcagg cgaacgacga ccgcgccttc gagtggctca tcagagcggg acgccgtgcg
     2461 atggagcgct acagctggcg gacggcagtc gagcgctggg aggcggcggc tgagttgctg
     2521 gaaggagacg acgcgcgccg gcgcgagttc ggctggctgc tcttcctgat gggaacgcat
     2581 ttgcgttatg cgagccgcga acgagcgctg gactacctgc gtgacgcgca atggatcgga
     2641 catgagatcg aagaccagtt gttgcgcgtc tgtgcgagcg ctggcatcgg ctttcagcgc
     2701 ctcttcagtg gccgcattga gggcggactg gaagacctgg tgcggagtat tgccgattat
     2761 gacagtctcg atcccgcgct gatcgagacc gtgccggaca tcgtcaaccg cgattctctg
     2821 ttgagcctga tggagaaccg gcgccagatg caggtgccgg ggctcgtgac gttcggccac
     2881 cagctcgacg cactgcgtct gagtgacgaa ctgcttgcgc gccccagaac gtacccgaac
     2941 aatgctgcaa tgacctcgat gggcaacgtc tatctcgggc gtgggttggc gctggcgagc
     3001 atggcccgac cagacgaagc tgccgatgcc ttcgcgcggg cccgtgagga gtttgagacg
     3061 acatccgaga cgaatccgta cgtgttcggc atcggcgcgt tcttcgcgtt gcatacggtt
     3121 gaattgccct atcatgcgga ccagatcgag cggcttgagg ccgtcgcgca tgttgggatc
     3181 gatgcctggg ttagcgcgcg ggagagcgcc accgaggggc gctcgacgcg ggtcgcgatg
     3241 ctggggatgc tgctgattca cggcgactgg gacgaagcgc gcgagattgc cgagatcggt
     3301 gtcgccggcg aggtcatcat ggtgctgtgg gacgagatgg tccgtgttct cggccttctg
     3361 aaccgtcatc agggaaggcc cgaccaggcc tgggagcagg tcgcgcgcta cttcccggac
     3421 ggaccgacag catctcccag cccaacctat gtcccgaccg cgacagcgct tcagcgtctc
     3481 gctgtcgcgc tggcgctgga cgcgggcgac gtgccgctgg cacacgactg gctcgtcgcg
     3541 catgatgcgt ggctggactg gtcggactcc gtcgccggcc acgcagacgg taccctgatc
     3601 tggg
//
LOCUS       DASUGJ010000438         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1318795, whole genome shotgun sequence.
ACCESSION   DASUGJ010000438 DASUGJ010000000
VERSION     DASUGJ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318795"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFV93_09660"
     CDS             <1..1180
                     /locus_tag="VFV93_09660"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165649.1"
                     /translation="GTYMQTVAIAWQLYLLTGSALQLGALGLFRAVPTITFALVGGAF
                     ADSRDRRRVLIITQALLGSFSAILAIATATGYASVALIYALTLLTATASCFDDPARAA
                     LIPNLVPRRHLAQALTLNILTRNIAAVVGPAAGGVAIATVGLSATYWLDAASFAAVIG
                     ALLAMRLRPKTPVVAGNGIQTVIEGLRFVRHNPVILSLMVLDFLATLFASALLLMPIF
                     ADEILGVGESGLGLLYSASAAGAVAGGLVLSALPVAHRPGRMVLGAVAVYGAAIVGFG
                     LAPSFPVALAALAASGAADTVSMTFRHAIRQLATPDALRGRVSAVASVFSGGGPELGN
                     FQIGVTARFIGIQPAVILGGLLTIGLASVVAIVSPRIRDYVIDAPEPEVESHANPALQ
                     "
     gene            1221..1523
                     /locus_tag="VFV93_09665"
     CDS             1221..1523
                     /locus_tag="VFV93_09665"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5165650.1"
                     /translation="MTRARIEIPQAAIEAFCRKHHIKQLALFGSVLRDDFTDESDVDV
                     LVEFKPGHTPGWEFFDMERELSAIIGRKVDLNTAGFLSPYFRDDVIREAEVRYDAA"
     gene            1510..1860
                     /locus_tag="VFV93_09670"
     CDS             1510..1860
                     /locus_tag="VFV93_09670"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX5165651.1"
                     /translation="MTLRDHTISMRRMLDHARMAIKHTTDRSLDDVERDEVLRMALVR
                     MVEVVGEAANRVPPEIRTLHPGVPWRQVIDTRNRLIHGHDTVDIRLVYEVIQHNLPPL
                     VAELERILEPQTES"
     gene            complement(2202..2435)
                     /locus_tag="VFV93_09675"
     CDS             complement(2202..2435)
                     /locus_tag="VFV93_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165652.1"
                     /translation="MLPVPVVPLQTGVPDWLALAVVVASVALAVLTLSLMLVRLRPAP
                     DEEHDAPVTVRDPVLLLSIVCVIALAVAAWLLR"
     gene            2620..>2879
                     /locus_tag="VFV93_09680"
     CDS             2620..>2879
                     /locus_tag="VFV93_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5165653.1"
                     /translation="MAELMPEVTPVTTHDTAREYRWEDDRGQRASANAVGEFQATTVR
                     ILIVDDDPTIRSVLEALLEDEGFMPVTAANGQEAVAIVRDEPP"
BASE COUNT          506 a          968 c          899 g          506 t
ORIGIN      
        1 cggcacctac atgcagacag tcgcgatcgc ctggcagctc tacctgctga ccggctcggc
       61 gctgcaactt ggcgcgctcg gcctcttccg ggctgtcccg acgatcacct tcgcgctggt
      121 cggcggcgcc ttcgccgact cacgcgatcg tcgccgggtc ctgatcatca cccaggcgct
      181 gctgggtagc ttctcggcca tcctcgcgat cgcaacggcg accggatacg cctcggtcgc
      241 cctgatctac gccctgacgc tgctgacggc gacggccagc tgcttcgacg acccggcccg
      301 ggcggcgctg atccccaatc tcgtcccgcg ccggcatctg gcccaggcgt taaccttgaa
      361 catcctgacg cgcaacatcg ccgccgtcgt cgggccggca gccggcggcg tcgcgatcgc
      421 gaccgttggc ctcagcgcga cctactggct ggacgcggcc tcattcgcgg ccgtcatcgg
      481 cgcgctgctg gcgatgcggc tacggccgaa gacgccggtc gtcgccggca acggcatcca
      541 gaccgtgatc gaggggctgc gcttcgtgcg ccacaacccg gtcatcctct cgctgatggt
      601 gctcgacttt ctggcaacgc tcttcgcctc ggcactgctg ctgatgccga tcttcgccga
      661 cgagatcctc ggcgtcggcg aatcgggcct gggcctgctc tactccgcca gcgcagccgg
      721 ggccgtcgcc ggcgggctcg tgctctcggc gctgccggtc gcccaccgac cgggccggat
      781 ggtgctgggc gcggttgcgg tctacggtgc tgccatcgtc ggcttcgggc tcgcgcccag
      841 tttcccggtc gcactggcgg cactggcagc ctccggcgcg gccgacacgg tcagcatgac
      901 cttccgccac gcgattcgcc agttggcaac gccggacgcg ctgcgtggcc gcgtctcggc
      961 cgtcgcgtcc gtcttctccg gtggcggtcc ggagctaggc aacttccaga tcggcgtcac
     1021 cgcccgcttc atcggcatcc agccggccgt catcctcggc ggcctgctga cgatcggcct
     1081 cgccagcgtc gtcgccatcg tctccccgcg catccgcgac tacgtcatcg acgcccccga
     1141 accggaagtc gagagccacg caaatcctgc gctacaatag gtccgatgac ctcgcccatt
     1201 ccgccatctg agcgacgcca atgacacgcg cgagaattga gattccccag gcagcaatcg
     1261 aagccttctg ccgcaagcac cacatcaaac agctggccct cttcggctcc gtcctgcgcg
     1321 acgatttcac cgacgagagc gatgtcgacg tgctggtcga gttcaagccg gggcacacgc
     1381 cgggctggga attcttcgat atggagcggg agctgtcagc cattatcggt cgaaaggtcg
     1441 atctgaatac ggcaggattc ctgagtccgt acttccgtga cgatgttatc cgcgaggctg
     1501 aggtacggta tgacgctgcg tgaccacacg atcagcatgc ggcgcatgct ggatcatgcg
     1561 cgaatggcca tcaagcatac gaccgacagg tcactcgacg atgttgaacg ggacgaagtg
     1621 ctgcgcatgg ctctcgtccg gatggtagag gtcgtcggcg aagccgcgaa tcgtgttccg
     1681 ccagaaatca ggacgctaca tccaggtgtg ccgtggcgac aggtcatcga tacccgcaat
     1741 cgcctcattc atgggcacga tacagttgac atccgtctcg tctatgaggt gatccagcat
     1801 aatctcccgc cgcttgtcgc ggagttggag cgcattctgg agccgcaaac cgagtcgtga
     1861 cttcatccag ttcacagccg ccggacaaaa cgagaggcgg ccctggggtg cgggtcgcct
     1921 cgggagggaa ggatggggtg aagtaggtag ggttgtgatg cgcggaccag cttcccggtc
     1981 cggaatctcg atgggtgggg accatcgtga ttcgctcata cttacgcaga gcgcgtgcca
     2041 ccggatcatc agtcttttcc ggcgaagaaa ttctgccgct gggccgctgc caccagcacg
     2101 acgcttgatg ccacgcgaaa tgtgttacaa atggggaaac aagcgacact attcgcgcaa
     2161 gccgggcggt gaatgcgcgt gatgcgtggc ccagccggcg cctagcgcag cagccaggcc
     2221 gcaaccgcca gcgcgatcac acagacaatg gaaaggagca gcaccggatc acgcaccgtc
     2281 accggcgcgt cgtgctcctc atcgggcgcc ggcctgaggc ggaccagcat cagcgagagc
     2341 gtcaggacgg ccagcgctac gctcgcaacg acgacggcca gcgccagcca gtcgggaacg
     2401 ccggtctgca acgggacaac cggaacgggt aacatcgaac acagtctcca gccaggaatc
     2461 gttcgctgaa cccggcctcc agccggccgg atccgctgac catcatcgta cacgctccgg
     2521 cttctggacg ccgccatggg tgctggcgtg cttcttgcgt agtacctgct gattcatcgc
     2581 gcacctgcac gcaatgtcga gtccgggcag agaatggtta tggctgagtt gatgccggaa
     2641 gtcacacctg tcaccacgca tgacacggcg cgcgagtatc gttgggagga tgaccgcggt
     2701 caacgcgcaa gcgccaacgc ggtaggggaa tttcaagcaa ccacagtgcg tattctgatc
     2761 gtcgatgatg accccaccat ccgctcggtc cttgaggcat tgctcgagga tgaaggcttc
     2821 atgcccgtca cagccgccaa tgggcaggaa gccgtcgcaa tcgtgcgcga tgagccacc
//
LOCUS       DASUGJ010000439         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1180518, whole genome shotgun sequence.
ACCESSION   DASUGJ010000439 DASUGJ010000000
VERSION     DASUGJ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180518"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1459
                     /locus_tag="VFV93_09685"
     CDS             293..1459
                     /locus_tag="VFV93_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5165654.1"
                     /translation="MTEIDRGALVEAIAELVRTPSINPSLVPGADGERAIAEQIAERL
                     RRTPGIEVELQDSGDGRPNVIASAGIGSGRTLMINGHIDTVGVAGMAEPYSGRVEGNR
                     LYGRGALDMKGAMGGALVLLEAVAGDPSFPGRLIVAFVTDEEHSSIGTQAVCREIERF
                     RPDAAIVVEPSGLNATIAHKGFAWAEIVTYGFAAHGSDWEHGVDAIAHMGRVIGALEA
                     HSRELLTRERHPIVGPPSLHLSLIGGGQELSSYPDRCRLEIERRTIPGESEEQVRAEL
                     QAILDRLATEDDQFKADLTMGLFRSPWSVSRDEQIVQVLAATYREDTGAEIVYDGAAG
                     WMDTALLGDAGVPAVVFGPDGDGAHAVVEWADLDSVERYTRILAKTAYAFCGAG"
     gene            complement(1493..1660)
                     /locus_tag="VFV93_09690"
     CDS             complement(1493..1660)
                     /locus_tag="VFV93_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165655.1"
                     /translation="MLLWIALILSVAALLTITIERLIARLGIRREDPTPSGLAMLAQR
                     LEQWSSKRRKK"
     gene            complement(1684..2118)
                     /locus_tag="VFV93_09695"
     CDS             complement(1684..2118)
                     /locus_tag="VFV93_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165656.1"
                     /translation="MATATSLRIEEPPEPMVPDSFHDRWNGRHWRWWALLAGVALTGL
                     ALALAAGLSAVREEDTAPLAFVIITLILFTISIAGSGMFDGLAARAISGGLARQPAPD
                     ARANRSSPSESESDRARRDRRTIRCGLAALPLAVTFLVLLFS"
     gene            2212..2970
                     /locus_tag="VFV93_09700"
     CDS             2212..2970
                     /locus_tag="VFV93_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165657.1"
                     /translation="MAGGDLLYIGAFIILPTAVLVSCIWALLLLRNGRLLPPRPVARY
                     EMAGEDVLADDEREDEPNPVEETGEHVVIAPAQPIAPPVPVAPTTAMSTGATVAPAAP
                     VISEPPPAERTQEMAAVPVEEPAGVQRSSAEAVGAEPAEIAEMTESEEAPAAAKATPD
                     AGPNVEPAPDLLLVPLDEPLVVQPDANGVMANGTNAAAAAVEADAANQTTPATPSRRA
                     PRRVGLPRQTNDGPPRSRPRTGQRRAARRSESEG"
BASE COUNT          508 a          959 c         1095 g          499 t
ORIGIN      
        1 ctaacgccac cttgacgttg aatcgcctga gtcgttgttt cgaatccatt gggatttcct
       61 acctggctat gctcacgtcg ctggttgcct cgaggatacg aatcttcgaa tcgccgttca
      121 cccgccaaaa agtcctgctt acaaaattgc gctgtgcaga tgtggactgt gtgagtcgaa
      181 gatttcggct tgctttcggc acggataccg tccgaacggc ggttgacttg tcctgcctat
      241 atcggcatcg tactggcgag atcgttcgag agcgagtcgg gaggggattt cggtgacaga
      301 gatcgatcgc ggggcgttgg ttgaggcgat tgcggagttg gtgcggacgc cgtcgatcaa
      361 tccatcgcta gtgccggggg cggatggcga gcgagcaatt gccgagcaga tcgccgagcg
      421 gttgcggcgg acgccgggga tcgaggtcga gctgcaggac tccggcgacg gccggccgaa
      481 cgtgatcgct tcggcgggga tcgggtcggg ccggaccttg atgatcaacg gacacatcga
      541 cacggttggc gtggccggga tggctgagcc gtactcgggg cgtgtcgagg gcaaccggct
      601 ctacgggcgc ggggcgctcg acatgaaggg ggcgatgggt ggagcgctgg tgctgctgga
      661 ggcggtggcc ggcgatccca gcttccccgg ccggctgata gtggcgttcg tgaccgacga
      721 ggagcactcc agcatcggga cgcaggccgt ctgccgtgag atcgagcggt tcaggcccga
      781 cgcggccatc gtcgtcgagc cgtcggggct gaacgcgacg atcgcccaca aggggttcgc
      841 ctgggccgag atcgtgacgt acggcttcgc ggcgcacggc agcgactggg aacacggcgt
      901 cgatgccatc gcccacatgg gacgggtgat cggcgcgctc gaagcgcaca gccgcgaact
      961 gctgacacgt gagcggcacc cgatcgtcgg gccgccgtcg ctgcacctct cgctgatcgg
     1021 cggcggccag gaactgtcgt cgtaccccga ccgctgccgg ctggagatcg agcggcgcac
     1081 gatccctggc gagtccgaag agcaggtgcg ggccgagttg caggcgatcc tcgaccggct
     1141 ggccaccgag gacgatcagt tcaaggccga tctgacgatg ggcctcttcc gctcgccgtg
     1201 gtcggtctcg cgcgacgagc agatcgtcca ggtgttggcc gcgacctacc gtgaggacac
     1261 cggcgccgag atcgtctacg acggtgccgc cggctggatg gacaccgccc tactcggcga
     1321 cgccggagtc ccggccgtcg tcttcggccc ggacggcgac ggcgcccacg ccgtcgtcga
     1381 atgggctgac ctcgactcgg tcgaacgcta cacccggatt ctggcgaaga cggcgtatgc
     1441 gttttgtggg gcgggatgag tgctgagtgc tgggtgacgc atcacgtatc actcatttct
     1501 tccgccgctt gctggaccac tgctcgagcc gttgggcgag catcgcgagg ccgctcgggg
     1561 tggggtcctc gcggcggatg ccgaggcggg cgatcaggcg ttcgatcgtg atcgtgagca
     1621 gggcggcgac ggacaggatg agcgcgatcc agagcagcat gtggaacctc tcgatggttg
     1681 gcgtcaggag aacagcagga cgaggaacgt gaccgccagc ggcaaggcgg ccagcccgca
     1741 gcggatcgtg cgacggtcgc ggcgggcgcg gtcgctctcg ctctcactgg gggacgatcg
     1801 attggcacgg gcgtcggggg ccggttggcg ggcgaggcca ccgctgatcg cgcgggcggc
     1861 cagaccatcg aacatgccgg agccggcgat gctgatcgtg aagaggatga gcgtgatgat
     1921 cacgaatgcc agcggcgcgg tgtcctcctc gcggacggcg gagagaccgg cggccagcgc
     1981 cagcgccagc ccggtcagtg ccacaccggc cagcagcgcc caccagcgcc agtgccggcc
     2041 gttccagcga tcgtggaatg aatctggcac cattggttcc ggcggctcct caattctcag
     2101 gctcgtcgca gtcgccaccg acaggttatc attggggcca gcatgtacgt acaggatccg
     2161 tcgacgactg gccggcctgc gacgggctaa cggcaggtca agggagtttc gatggccgga
     2221 ggcgatctgc tctacatcgg agcgttcatc attttaccga cagcggtcct ggtgagctgt
     2281 atctgggcgc tgctgctgct gcgcaacggc cggctgctac cgccccgtcc ggtcgcgaga
     2341 tacgagatgg ccggcgaaga cgttcttgct gacgatgaac gtgaagacga acccaacccc
     2401 gttgaagaga ccggcgagca tgtcgtgatc gctcccgccc agccgatcgc tccgcccgtg
     2461 ccggtcgcgc cgactaccgc gatgtcgacc ggggcaacgg tggcgccagc cgcgccggtc
     2521 atctctgagc cgccaccagc cgaacggacg caggagatgg cggctgtgcc tgttgaggag
     2581 ccggctggcg ttcagcgttc gtctgccgag gctgtgggtg ccgagccagc tgagattgct
     2641 gagatgacag agtccgaaga ggctcccgct gcggcgaagg cgacgccaga tgccggcccg
     2701 aatgtcgaac cggcgccgga tctgctactc gtgccgctcg acgagccgct ggtggtgcag
     2761 ccggacgcga atggtgtgat ggcgaacggg acgaacgcgg cagccgcagc cgtcgaggcc
     2821 gatgcggcga accagacgac accggcgaca ccgtcccgtc gtgcgccccg tcgcgttggc
     2881 ctgccgcgcc agacgaacga cggcccacca cgctcgcgtc cccgcacagg ccagcgccgc
     2941 gccgcccgcc gcagcgagag cgaaggctga tcgctcgccc tcaatccaca agcccaatcg
     3001 tccaatcgcg taggggcgat atcgcaatat cgccctgctc cccgcccgac gtgtctgggt
     3061 g
//
LOCUS       DASUGJ010000440         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1737164, whole genome shotgun sequence.
ACCESSION   DASUGJ010000440 DASUGJ010000000
VERSION     DASUGJ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737164"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VFV93_09705"
     CDS             complement(<1..1241)
                     /locus_tag="VFV93_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5165658.1"
                     /translation="MNSGRRPGTLPIRRWLALALLITFLVPVCTTITFAVSTVRGNPH
                     DKQDDAVDLIRDASAQWTDPAWQEATAAHLAHDDVAFVLIEDGREIYRSAADPLVGTD
                     GEHDPRTVERVTIDGSGSSQVAYVYADEDIGPPDELPVQYLPLVVIGSLALTLTGIAW
                     FFGRTMLRPLAATSRAARQIAAGDLDISLPKSRVREVAEVSDAFESMSVALRNSLEEQ
                     AELEQERRLFIGAVAHDLRTPLFALRGYLEGIESGVASTPEQRARYLGVAREKADALE
                     LLISDLFDFSRLEYLDQAPNLEPIDLTALLHKLVVGIRPRATAKNVTIAVEFEAKPCR
                     VDADSHLLTRAIENLLDNALRFTPNGGTVRVTCEATSAVAAFSIADSGPGIAPADLPN
                     LFAPLYRGESSRNRRTGGAGLG"
     gene            complement(1238..1933)
                     /locus_tag="VFV93_09710"
     CDS             complement(1238..1933)
                     /locus_tag="VFV93_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5165659.1"
                     /translation="MEQQPTVLVVDDEDDIVTLMRDFLEAAGYRVLTADDGPAGLNIL
                     RREPVDCLLLDVMMPGISGFEVLRRIRETRDVPVLFLSAKQEDSDKIRGLGLGADDYI
                     VKSATPGEVVARVRAVLRRVGRGEAQAAAVLDFGRLVIDLRAHEVLVEGEPVSLTARE
                     FELLQLLAESPRQVFTREQLFERLWGAYGDRHTLTVHIGRLREKIEEDPASPRYIVTV
                     WGVGYRFEGERRS"
     gene            2220..2489
                     /locus_tag="VFV93_09715"
     CDS             2220..2489
                     /locus_tag="VFV93_09715"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX5165660.1"
                     /translation="MSIDSLASSLVLLNANCPWGDGGYWWIGRIIMFLIFIVLLFCFF
                     WFMRRNAWRHHHQDPIERAQGILAERYARGEIDSEEYRDRLSQLR"
     gene            2568..>3178
                     /locus_tag="VFV93_09720"
     CDS             2568..>3178
                     /locus_tag="VFV93_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5165661.1"
                     /translation="MTITTEQTRTATLNRDARTSNGIIEATNVVKTYETGKITVNALR
                     GVNFSVERGDMVAIMGPSGCGKTTLLNCLSGLDTINAGQILIDGTDLAVLPDNARTEY
                     RAENMGFVFQFYNLLPVLSAAENVELPLLVSGVKPKEARQRALAILERVGLGQWAHHK
                     PAELSGGQRQRVTIARALVNEPAIVWADEPTGDLDSETADDIIG"
BASE COUNT          563 a         1068 c         1028 g          519 t
ORIGIN      
        1 ccgaggccgg cgccaccggt gcgacggttg cgcgagcttt cgccgcggta gagtggggcg
       61 aacaggtttg gcaggtcggc cggggcgatg ccggggccgc tgtccgcgat tgagaacgcg
      121 gcaacggctg atgttgcctc acaggtgaca cggacagtcc caccgttcgg cgtgaagcgc
      181 agcgcgttgt cgagcaggtt ctcgatcgcg cgggtcagca ggtggctatc ggcgtcaacc
      241 ctgcacggtt tggcctcgaa ttcgaccgcg atcgtgacgt tcttggcggt cgcgcggggc
      301 cggatgccaa cgaccagctt gtgtagcagc gcggtcaggt cgatcggctc gagattcggc
      361 gcctgatcga gatattccag ccggctgaag tcgaacaggt cgctgatcag cagttcgagc
      421 gcgtcggctt tctcgcgggc gacaccgagg tagcgggccc gctgctcggg tgtgctggcg
      481 acgccggact cgatcccctc caggtagcca cgcagggcaa agagcggcgt ccgcagatcg
      541 tgggcgacag cgccgatgaa cagccgccgc tcctgctcca gctcggcctg ctcctccagc
      601 gaattccgca gcgcaacact catcgactcg aacgcgtcac tgacctcggc gacctcgcgg
      661 acgcgcgact tcggcagcga gatgtccagg tcgccggccg cgatctggcg ggcggcgcgg
      721 ctggtggcgg ccagtgggcg cagcatcgtc cggccaaaga accaggcgat gccggtcagc
      781 gtcaacgcca gcgaaccgat gacaaccagc ggcagatact ggaccggcag ctcgtccggt
      841 ggtccgatat cctcatccgc gtagacatag gcgacctgtg acgaccccga gccgtcgatc
      901 gtgacccgct cgaccgttcg tgggtcgtgc tcgccgtcgg tgccgacaag cgggtcggcc
      961 gcgctacggt agatctcgcg gccgtcttcg atcaggacaa aggcgacgtc atcgtgggcc
     1021 aggtgggcgg ccgtggcctc ctgccaggcg gggtcggtcc attgtgcgct cgcgtcgcga
     1081 atgaggtcga cggcgtcgtc ctgcttgtcg tgcgggttgc cacgcacggt gctgaccgcg
     1141 aacgtgatcg tcgtgcagac cgggaccagg aacgtgatca gcagcgccaa cgccagccag
     1201 cggcgaatcg gcagcgtgcc tggccggcgg ccgctgttca cgaccggcgc tcaccttcga
     1261 agcggtagcc gacgccccag acggtgacga tgtagcgcgg gctggccggg tcttcctcga
     1321 tcttctcccg cagccggccg atgtggactg tcagcgtgtg ccggtcgccg taggcgcccc
     1381 agaggcgctc gaagagctgc tcgcgggtga agacctgacg gggcgattcg gccagtagtt
     1441 gcaacagctc gaactcgcgg gccgtcaacg agaccggctc gccttcgacc agcacctcgt
     1501 gggcgcgcag gtcgatcacc agccttccga agtcgaggac ggccgcagcc tgggcctcac
     1561 cacgcccgac ccgtcgcaga acggcccgca cccgcgcgac cacctcgccg ggcgtcgccg
     1621 acttgacgat gtagtcgtcg gcgccgagcc ccagcccgcg aatcttgtcg ctgtcctcct
     1681 gcttcgcgct gagaaagagg accggcacat cgcgcgtctc gcgaatccgc cgcaggacct
     1741 cgaagccgct gatacccggc atcatcacgt ccagcaacag gcaatcgacc ggctcgcgcc
     1801 gcaggatgtt cagcccggcc ggcccatcat cggccgtcag aacgcgatag ccggccgctt
     1861 ccagaaagtc ccgcatcagg gtaacgatgt cgtcctcatc gtcaacgacc aggacggttg
     1921 gttgttgttc catcggggtg attcctcttc tgtcgggatt cgggattcgg gggtgtcgga
     1981 cgaacatcgt cccttcaata cccccgaacc ccgagtcccg aatcccgacc gtcccacgtc
     2041 tctgtcctca gtatgaacga cctctcgacc ccattgccac gcccgaagcc ccatctgacg
     2101 aagctgctac aggctgttga gcaagtgttg agatttgccc ccacagcgcg ttgaccttgc
     2161 cctccgacaa tcagaacaca gccgtcacgg cggcgaatct gacggaaggg agagacgcaa
     2221 tgtcaatcga tagcctggca agcagcctcg tgctcctcaa tgccaactgc ccctggggag
     2281 acggtggtta ctggtggata ggaaggatca tcatgtttct gatcttcatc gttctgctgt
     2341 tctgcttctt ctggttcatg cgccgcaacg cgtggcgaca tcaccatcag gacccgatcg
     2401 agcgtgcgca gggcatcctc gccgagcgct acgcccgggg cgagatcgac agcgaagagt
     2461 accgcgaccg cctcagccag cttcgctgat ccgcgaccga ccacgaccgg ctcggcatga
     2521 ctccgggccg gtcgcccgtc gccagtaacg atgagagagg acccacgatg acaatcacga
     2581 ccgaacaaac acgaacagcc acgctgaacc gggacgccag gaccagcaac ggcatcatcg
     2641 aagcgacgaa tgtggtgaag acgtacgaga ccggcaagat caccgtcaat gcgctcagag
     2701 gcgtcaactt cagcgtcgaa cgcggcgaca tggtcgccat catggggccg tccggctgcg
     2761 gcaagacgac gctgctcaac tgcctgtcgg gcctcgacac catcaacgcc ggccagatcc
     2821 tgatcgacgg caccgacctg gctgtcctgc ccgacaatgc ccgcaccgag taccgcgccg
     2881 agaacatggg cttcgtcttc cagttctaca acctgctccc cgtcctctcc gccgccgaga
     2941 acgtcgagtt gccgctgctg gtctcgggtg tcaagcccaa ggaagcgcgc cagcgtgcgc
     3001 tggcgatcct ggagcgggtc gggctggggc agtgggccca tcacaagccg gccgagctct
     3061 ccggtggcca gcgccagcgg gtgacgattg cccgtgccct ggtcaacgag ccggccatcg
     3121 tctgggcgga tgagccgacc ggggatctgg actcggagac ggccgatgac atcatcgg
//
LOCUS       DASUGJ010000441         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_902978, whole genome shotgun sequence.
ACCESSION   DASUGJ010000441 DASUGJ010000000
VERSION     DASUGJ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902978"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..666
                     /locus_tag="VFV93_09725"
     CDS             244..666
                     /locus_tag="VFV93_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165662.1"
                     /translation="MNSTPSPTDSPTGLPVCAPEDYALSLELPGAAAGSVGYRVDAVG
                     QSAACWLDTVLTITFVGADGQPLPIDGNGMTLPLVGDLPDGAAAGVLWSNWCGERESI
                     RVELATPDHIVQSDQVTVPGCNGPGQPSTVSPIPDDGL"
     gene            complement(672..1403)
                     /locus_tag="VFV93_09730"
     CDS             complement(672..1403)
                     /locus_tag="VFV93_09730"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5165663.1"
                     /translation="MTKRPERMRDRAPSANPVDPLPPGLWPERSAIEGRYMRLEPLDA
                     RVHAAELYAASHGSDEAVRVWDHLAVGPFPSLDAFATYQRGCSASADPVFYAIRDLKT
                     GVASGVASYLNIVPAFGSIEIGHIWFGPALQNTPAATEALYALIHHAMDDLGYRRMEW
                     KCNALNSGSRSAAVRLGFAFEGIFYNHMIAKGRNRDTAWFSIIDDEWPALRECFEDWL
                     APDNFDAGGRQRSSLGDLTAAVRAR"
     gene            complement(1493..2413)
                     /locus_tag="VFV93_09735"
     CDS             complement(1493..2413)
                     /locus_tag="VFV93_09735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5165664.1"
                     /translation="MTQTTPGPSVRAVTVDDIPAIREVLFAALEAGELAGTTARDLNH
                     ELDLIPVVPGGMLVSLAGDEVTGFFDAEYPLLAVHPRHRRQRHATRLVERAIAEAPAH
                     GLAEVDLAPPLGNRPAEAFAASLGFAYRSSLWQLRLDPATDVPPPRFPAGFEPRAIQP
                     GPDDEAYLDLLRDSFADHPLQLNVSLDIIRLAHSRPSFNPSNVAVVANPDDAGQLIAF
                     CRVILTEEDTGQHAEIGHLGVLPGYRGRGLGRELLRWGVHYLRSRGVAEILLAVESRN
                     NRALRIYERAGFVAVQEWPRWAKSGPSPAL"
BASE COUNT          442 a          826 c          890 g          389 t
ORIGIN      
        1 gggcttgagg tcgaccagca acacgcctgg cgtcgttccc ccatgtgact ggcccaatca
       61 gccctcaacg ctgggacagc tgagttcccc cgacatcatc atgaccccgc ccgcgtcgcc
      121 agcgacggaa ccggcagcga atccgacacc gacgcccatc acgcgcaacc aacgacagcg
      181 ccgatcacgc caacggcatc gctgctcgca accccgtcag caacggcaac gaccgtgccg
      241 gccgtgaact cgacgccaag tccgaccgat tccccgaccg gcctgccggt ctgtgctccc
      301 gaggactacg cgctgtcact cgagttgcca ggggcggcgg ccggcagcgt cggctaccgc
      361 gtcgacgccg ttggacaatc cgctgcctgc tggttggaca ccgtgctgac gatcacgttc
      421 gtcggcgcgg acggtcagcc actgccgatc gacggcaacg ggatgacgtt gccgctggtg
      481 ggagatctgc cggacggagc ggcggccggc gtgctctggt ccaactggtg tggcgaacga
      541 gagtcgatcc gtgtcgagct tgccacgccg gaccacatcg ttcagagcga ccaggtcacg
      601 gtgcccggct gcaacggacc gggccaacca tcgaccgtgt ccccgatacc cgacgatggt
      661 ttgtaggcca gtcagcgcgc ccggacggca gccgtcaggt cgccgagcga gctgcgctgg
      721 cggccgccgg cgtcgaagtt gtcgggggcg agccagtcct cgaagcactc gcgcagggca
      781 ggccactcgt cgtcgatgat cgagaaccag gccgtgtcgc ggttgcggcc cttggcgatc
      841 atgtggttgt agaagatgcc ctcgaaggcg aagccgagcc ggacggcggc gctacgcgat
      901 ccgctgttga gcgcgttgca cttccactcc atcctgcggt agccgaggtc gtccatcgcg
      961 tggtggatca gtgcgtagag cgcctcggtc gcggccggtg tgttctggag ggccgggccg
     1021 aaccagatgt ggccgatctc gatcgagccg aaggccggga cgatgttgag gtagctggcg
     1081 acaccgctgg caacaccagt cttcagatcg cggatggcgt agaagaccgg gtcggcgctg
     1141 gccgagcagc cgcgctggta ggtggcgaac gcgtccagcg acgggaacgg cccaaccgcg
     1201 agatgatccc agacgcgaac ggcctcgtcg ctgccgtgcg aagcggcata gagctcggcc
     1261 gcgtggacgc gcgcgtcgag cggttccagc cgcatgtagc ggccctcgat ggcgctgcgc
     1321 tccggccaga ggccgggcgg cagcggatcg acaggattgg cggacggcgc gcggtcgcgc
     1381 attcgctctg gtcgcttcgt cacggttacg gagtcctttc agacaatgaa cgctgcccgc
     1441 tgtccgggca gcgttcattg ttggcgaggg tggcccggat ggcacgggcc aattacaacg
     1501 cgggggaagg gccactcttc gcccagcgtg gccactcctg aaccgcgacg aaaccggcgc
     1561 gctcgtagat ccgcagcgca cggttgttgc ggctctcgac agcgagcagg atctcagcga
     1621 cgccgcgcga gcgcaggtag tggacgcccc agcgcagcag ctcgcggccg aggccacgtc
     1681 cacgatagcc aggcaggacg ccgaggtggc cgatttccgc atgctggcca gtatcttcct
     1741 cggtgaggat cacccggcag aaggcgatca gctgcccggc gtcatcggga ttcgcgacga
     1801 ccgcgacgtt agatggattg aagctcggcc gggaatgggc cagccggatg atgtcgagcg
     1861 agacattgag ctggagcgga tggtccgcga acgagtccct gaggaggtcc aggtacgctt
     1921 cgtcgtccgg accgggctgg atcgcgcgcg gttcgaagcc ggccgggaag cgtggcggcg
     1981 ggacgtccgt tgctggatcg agtcgcagct gccagagact ggaacgatag gcaaagccga
     2041 ggctggcagc gaacgcctcg gccggtcggt tgcccagcgg cggcgccaga tccacctcgg
     2101 ccagcccgtg cgccggagcc tcggcgatgg cgcgctcgac gagccgcgtg gcatgccgtt
     2161 ggcgacggtg gcggggatgg acggccagca gcggatattc agcatcgaag aagcctgtca
     2221 cctcatcgcc agccagactg acgagcatac cgcccgggac gaccgggatc aggtcgagct
     2281 cgtggttcag atcacgcgct gtcgtgcctg ccagttcgcc ggcttccagc gccgcgaaca
     2341 gcacctcgcg gatggccggg atgtcgtcaa cggtgactgc gcgaacggat ggcccgggcg
     2401 tcgtctgcgt catcacacgg ccttcctcag ccgaagatcc actgccggag gggatgatta
     2461 tcattgcctc atcagcagag atgcaacagg cgtgagccga aggatctctc ctggggtaac
     2521 tgatcggcca acgcaggaga gatcctt
//
LOCUS       DASUGJ010000442         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_903004, whole genome shotgun sequence.
ACCESSION   DASUGJ010000442 DASUGJ010000000
VERSION     DASUGJ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903004"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFV93_09740"
     CDS             <1..289
                     /locus_tag="VFV93_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5165665.1"
                     /translation="GRRQGRMQRLVGRLRGAESAHEDALATVIRGEDAATVRRLLAGL
                     PARDRELVLLRYSAGLNSSEIAEALGMSSGAARVRLMRVLQRLAAELGDDR"
     gene            286..>2084
                     /locus_tag="VFV93_09745"
     CDS             286..>2084
                     /locus_tag="VFV93_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5165666.1"
                     /translation="MSDANEFQVDPRLDALLARALGRGSLSRQQRQQHLERLGIMPHL
                     GGSAGRWRAPVVAGRPAASAAPAPAALPSKRRRRQWLELGAAVLAVVLVASLLVALFR
                     DWNGDEGDNQLAASPEATATPPETREQLYVVSQPVSIDGDSIQMTGRVSAIDPATGDE
                     FWSVEAAPRLDSVLSPDGRRLFVADSGPDGDHLYALDTQTGRELWRQPVENRQWLKPG
                     GSYSTLGVSPDGSTVSVYACEGCTGEIRSTEGRNWLARFDAGSGQPRPDLEIDACRGK
                     TFLGPAGVIYVICDAGSDGQANRLQIVDTASGQPVTLTYDVRLDGGFPETTGVFASVA
                     SPDGHLLYLAAGNRLTVIDMTRHAATRIQLDAGINVAQRMPFLTLSPDGGRLYIGSLD
                     AAAYAAGNAEVNRVDAYDTASWEHLGLLSMGSPLTHADIAAGPDGDAVYGVFAYHPKT
                     DDSVIIESTIMRLGLDAEPVNVATRPNEEVLYLFAGTVEVDPDATPLRTPTPEPSAHE
                     RLYAVSHIRSTANSSVSGRVTAYDAQTGEIDYWIDTDINPDAVLSPDGSRIYIVTGGI
                     ISSGDTVNTTVQATEASSGDDLWSVHADLRLPWV"
BASE COUNT          324 a          716 c          728 g          316 t
ORIGIN      
        1 gggacggcga caggggcgga tgcaacggct ggtcggccgg ctgcgcggcg cggagagcgc
       61 ccacgaggac gcgctggcga cggtcattcg cggcgaggac gcggccaccg ttcggcgctt
      121 gctggccggg ctaccggcgc gggatcgtga actggtgctg ctgcgctaca gcgccgggct
      181 caacagcagc gagatcgccg aggcgctggg gatgtcgagc ggggcggcgc gggtgcgcct
      241 gatgcgcgtg ctgcaacggc tggcggctga gttgggagac gaccgatgag cgacgccaac
      301 gagttccagg tcgatccacg actcgatgcg ctgctcgccc gcgcgctggg ccggggcagc
      361 ctctcgcgcc agcagcgcca gcagcatctc gaacggctgg gtatcatgcc acacctcggc
      421 ggcagtgccg gacgctggcg ggcgccggtt gtcgccggcc gccctgccgc ttcggcagcg
      481 cccgctccgg ccgcgctacc gtcgaagcgc cgtcgtcgcc agtggctgga gcttggcgcg
      541 gccgtcctgg cggtggtgct ggtcgcctct ctgctggtgg ccctcttccg cgactggaac
      601 ggcgacgagg gcgataacca actggccgcc tccccggagg cgaccgcgac gccacctgag
      661 acgcgcgagc agctctatgt cgtcagccag ccggtgtcga tcgatgggga ttcgatccag
      721 atgacgggcc gtgtcagcgc gatcgacccg gcgacgggtg acgagttctg gtcggtcgag
      781 gcggcgccac ggctcgatag cgtcctctcg cccgatggcc ggcggctctt cgtcgccgac
      841 agcggcccgg acggcgacca cctctatgcg ctcgacaccc agaccggccg cgaactctgg
      901 cggcagccgg tcgagaaccg tcagtggctc aagccgggtg gatcgtactc aacgctgggt
      961 gtgtcaccgg atggcagcac cgtctcggtc tatgcctgcg agggctgcac gggtgagatc
     1021 cgctcgaccg aggggcgcaa ctggctggcg cggttcgacg ccgggagcgg ccagccgcgg
     1081 cccgatctgg agatcgatgc ctgccgcggc aagacgttcc tcggaccggc tggcgtcatc
     1141 tatgtcatct gtgacgccgg ctccgacggg caggccaaca ggttgcagat cgttgacacg
     1201 gccagcggcc agccggtcac gctgacctat gacgtgcgcc tggacggcgg cttccctgag
     1261 acgaccggtg tctttgcctc ggtggcctca ccggacggcc acctgctcta cctcgctgcc
     1321 ggcaaccggc tgaccgtcat cgacatgacc cgccacgccg cgacgcggat ccagcttgat
     1381 gcaggtatca acgtcgccca gcggatgccg ttcctgacgc tctcgccgga tggcgggcgg
     1441 ctttacatcg gcagtctcga cgcggcggcc tacgcggcag gcaacgccga ggtcaaccgc
     1501 gtcgatgcct acgacacagc cagctgggag catctgggcc tgctctcgat gggatcgccg
     1561 ctcacccacg ccgacatcgc cgccgggccg gatggtgacg cggtctacgg cgtcttcgcc
     1621 taccacccga agacggacga cagcgtcatc atcgagtcga cgatcatgcg gctcgggctg
     1681 gatgccgagc cagtgaacgt cgcgacccgg ccgaacgagg aggtgctgta cctcttcgca
     1741 ggaacggtcg aggtcgatcc ggacgcgacc ccgctccgga cgccaacgcc ggaacccagc
     1801 gcccacgagc ggctctacgc cgtcagccac atccgctcta ctgccaacag tagcgtgagc
     1861 gggcgtgtca ctgcctacga cgcgcagacc ggcgagattg actactggat cgacaccgac
     1921 atcaaccccg atgccgtact ttcaccggat gggtcgcgga tctacattgt cacgggcggg
     1981 atcatcagca gtggcgacac ggtcaatacg acggtccagg cgaccgaggc atccagcggt
     2041 gacgacctct ggagcgtgca tgccgatctc cgcctgccct gggt
//
LOCUS       DASUGJ010000443         4703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1457864, whole genome shotgun sequence.
ACCESSION   DASUGJ010000443 DASUGJ010000000
VERSION     DASUGJ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457864"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VFV93_09750"
     CDS             complement(<1..1202)
                     /locus_tag="VFV93_09750"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165667.1"
                     /translation="MSEGDGRSRLPVFVLKHRDYRLLWIGQFVSTLGTQMHTVALSWQ
                     VYDLTGSIFQLGLLGLVRAASLMGTSFLGGTLADSRNRRNLMLVTQSIMLALTSSLAV
                     LTFFDIVNIWMLYAFAAAIAATSAFDGPARQALIPNLVPRPELGAAISLQTLAMSTAR
                     MVGPAVGGVTVGVIGVAATYALDSASFLAVILALLLMRTPMVAPVLTSRGLEAVREGL
                     RFIVATPVIWGIMLLDFLATLVGSTVGLAPVFAKDVLHAGPQGLGLLLSAPAAGAVIG
                     AVIVSVMPQFERPGRMVVVSVLVYGVCLALFGMSGSLLPALLALAGAGAADSVSVAMR
                     QMVRTIATPDELLGRVAAAHSALAMGGPRLGEFQSGMTAAMVGPRAAMMLGGTGCVLI
                     ALAIGRFIP"
     gene            1399..2682
                     /locus_tag="VFV93_09755"
     CDS             1399..2682
                     /locus_tag="VFV93_09755"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165668.1"
                     /translation="MALSNHLRRRPQAAIVEAPSASTIDNPVGRFAALDVPAFRNYFI
                     ATVVSNIGSWMQIVAQGWLVLSLTDSPFYLGLVGLVRAVPTILLSLIGGVLADRFDRR
                     RILIVTQSVAMISSVLLAVLVIADIVTVWHILAISFVSSVFFAADNPTRQALVPDLVG
                     RERLTSAIGLNSAAWNGAAVIGPSIAGILIAAVGVAGAFALNAASYLAVMGVVITMPE
                     LPRYASAKRSVAGQLVDGMRYILDQRAIWGILLLIAIPSLGARPYIQMMPVFARDVLG
                     MGATGYGVLMAASGIGALVGALVVTSLGSSTRRGAILLAVTAGLGVALIAFSSSHWLL
                     PSLALVVVVGGASTLMMSLANAVLQGLVPGEMRGRVMSVYSLIAAGFMPLGSMLLGSL
                     GSVIGVPLAVGLGGALTIATAALSARLLTDIRAVE"
     gene            complement(2764..3261)
                     /locus_tag="VFV93_09760"
     CDS             complement(2764..3261)
                     /locus_tag="VFV93_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5165669.1"
                     /translation="MTTTTPLSLPDRDGLKAPFAMLHHVAMITNDMEKTVAFYRDVLG
                     SEIALAHRLNRDDEARHYFITVAPNCVFAFFEFTDAELPEFQEATVHKSGRFLDHICF
                     FVESAEELDGWHERVTAAGVPVHGYAPGNNLFFPDPNNIIFQVSVGKPGPMGFPVHDD
                     PRPAY"
     gene            3436..3693
                     /locus_tag="VFV93_09765"
     CDS             3436..3693
                     /locus_tag="VFV93_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165670.1"
                     /translation="MEAPTSGFDAERVAAAHHRNTLQRNVQNRIMREKARLLHDWTTA
                     GGSEDDFEEVWPSIRAQLGQIRVMEIGDKARARSLTAFNRR"
     gene            complement(3790..>4703)
                     /locus_tag="VFV93_09770"
     CDS             complement(3790..>4703)
                     /locus_tag="VFV93_09770"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165671.1"
                     /translation="VTVVLMGIANAFSYPAHSSLLPSLVPVRTLQSANAINSMMGNLS
                     RVAAPTVSGLIVDSAGVAAALLLASGIYLPAAGLVLLVPLLLSAEGVSATRGPLQPAA
                     RPSFRSDIGDAVGYIRTNPLLRSALVNDIVPYLFGMAHIALLPAIASDTLDGGATMLG
                     LLFAVSGVGAMFGTLTAGALTGRELRGRTIWIATIGFGAGLLIVAAGSSASVIMPGLF
                     VTGYFQMVYIIQNDTLVQTFAEDRFRGRAVAAQSMINGLMPIGFLMLGAIAELSSVPI
                     AFAVAGGALIISGVATRMFRPVMSGLR"
BASE COUNT          837 a         1695 c         1400 g          771 t
ORIGIN      
        1 gggatgaacc tgccgatcgc cagcgcgatc aggacgcaac ctgttccacc gagcatcatc
       61 gctgctcgtg gtccaaccat cgccgctgtc atcccgctct ggaactcacc cagccgcggg
      121 ccgcccattg cgagtgcgga gtgggccgcc gcgaccctgc cgagcagctc gtccggcgtc
      181 gcaatcgttc gcaccatctg gcgcatcgca acactgaccg agtcagccgc gcctgccccc
      241 gccagtgcca gcagcgccgg tagcaacgag ccgctcatcc caaacagcgc caggcagaca
      301 ccgtagacga gtaccgagac gaccaccatc cgccccggcc gctcgaactg cggcatgaca
      361 ctgacgatca ccgctccgat gacggcacct gccgccggag ccgacagcag cagccccaac
      421 ccctgcggcc cggcatgcag gacatccttg gcgaacaccg gcgccagccc gaccgtcgag
      481 ccgaccagcg tcgccaggaa atccagcagc atgattcccc agatgaccgg cgtcgcgacg
      541 atgaagcgca gcccctcgcg aacggcttcc agcccgcgcg acgtcaggac cggcgcgacc
      601 atcggcgtcc gcatcagcag cagtgccagg atcacggcca ggaacgaggc cgaatcgagc
      661 gcgtaggtag ccgcgacacc gatcacgccg acagtaaccc caccaaccgc cggcccgacc
      721 atgcgggccg tgctcatcgc cagcgtctgg agcgagatgg ccgccccaag ctccgggcgc
      781 ggcaccagat tcgggatcaa cgcctgccgc gccggcccat cgaacgcgga cgtcgcggca
      841 atcgccgccg cgaacgcgta cagcatccag atgttcacga tgtcgaagaa cgtcaacact
      901 gccagcgagc tcgtcagcgc cagcatgatc gattgcgtca ccagcatcag gttgcgtcga
      961 ttgcgcgaat ccgccagtgt cccgcccagg aacgacgtcc ccatcagcga cgccgcccgt
     1021 accagaccca gcagaccaag ctgaaagatc gaccccgtca ggtcatacac ctgccacgac
     1081 agggcaaccg tgtgcatctg agtcccgagc gtcgagacga actggccaat ccacagcagc
     1141 cggtagtcac gatgcttgag cacaaacacc ggcaaccggc tgcgaccgtc accctcgctc
     1201 attgccaccc gaccgcgaac acgtgttcca tccccacttc cggccagccg ttatgccaca
     1261 gatttcatac ctgcgacaca tcctacgaat cgtttcgcgg caaatgattt gcatgacgct
     1321 gttcggttca ggcgtgcgca gaatcaactg tcgagtatca tccccgcaaa atccgtgacg
     1381 agcaaggaac gagacagcgt ggcactctcc aaccatctcc ggcgacggcc gcaggccgcg
     1441 atcgtcgaag cgccgtcagc gagcacgatc gacaaccctg tcggccgctt cgccgcgctc
     1501 gatgttcccg ccttccgcaa ctacttcatc gccaccgtcg tctcgaacat cggctcgtgg
     1561 atgcagatcg ttgcccaggg ctggctggtc ctctcgctga ctgactcgcc cttctatctc
     1621 ggcctcgttg gactcgtccg ggctgtcccg accatcctgc tctcgctgat tggcggcgtc
     1681 ctggccgacc gtttcgaccg ccgccggatc ctgatcgtca cccagtcggt cgcgatgatc
     1741 tcgtcggtcc tgctggccgt cctcgtcatc gccgatatcg tcacggtctg gcatatcctg
     1801 gcgatctcat tcgtctcgtc agtcttcttc gccgccgaca acccgacccg ccaggcgctg
     1861 gtgcctgacc tcgtcggccg tgaacgcctc acgagcgcga ttggcctcaa ttcggctgcc
     1921 tggaacggag cggccgtcat cggtccgtcg atcgcgggca ttctgatcgc cgccgtcggc
     1981 gtcgccggcg cgttcgcact caacgccgcc agctatctgg ccgtcatggg cgtcgtcatc
     2041 acgatgccgg aactgccccg ctacgccagc gccaagcgct cggttgccgg ccagctcgtt
     2101 gacggcatgc gctacatcct cgaccagcgc gcgatctggg gcatcctctt gctgatcgcg
     2161 atccccagcc tcggcgcccg cccatacatt cagatgatgc ccgtcttcgc tcgtgacgtg
     2221 ctcgggatgg gcgccaccgg ctacggcgtc ctgatggccg ccagcggcat cggtgcgctg
     2281 gtcggcgcgc tggtggttac ctcactcggc tcctcgacga ggcgcggcgc gatcctgctg
     2341 gccgtcacgg ccggcctcgg agtcgccctg atcgcgttct cgtcgtcgca ctggctgcta
     2401 ccgtcgctgg cgctggtcgt cgtcgtcggt ggcgcctcga cgctgatgat gtcgctcgcc
     2461 aacgccgtcc tccagggtct ggtcccaggc gagatgcgcg ggcgcgtcat gagcgtctac
     2521 tcgctgatcg ccgctggctt catgccactc ggctcgatgc tgctcggctc gctcggctcc
     2581 gtcatcggcg tcccactggc cgtcggcctc ggcggcgccc tcacgatcgc caccgccgct
     2641 ctctcggcgc ggttgctgac tgatattcgg gctgtcgagt gaccgggatt cgggaaccgg
     2701 gagcatcaag cggagcacac ccgtcaaaca accacgagtc cgagtcccga ttcccgaacc
     2761 ccatcaatac gctggccgcg gatcgtcgtg caccgggaac cccatcgggc cgggcttgcc
     2821 gacgctgacc tggaagatga tgttgttcgg gtcggggaag aacaggttgt tgcccggtgc
     2881 gtagccgtgg accggcacac cggccgccgt cacccgctcg tgccagccgt ccagctcctc
     2941 ggcgctctcg acgaagaagc agatgtgatc caggaaccgg cccgacttgt ggaccgtcgc
     3001 ctcctggaac tcgggtagct cggcgtcggt gaactcgaag aaggcaaaga cgcagttcgg
     3061 cgcgaccgtg atgaagtagt gccgcgcctc gtcatcgcgg ttcagccggt gcgccagcgc
     3121 gatctcactc ccgagcacat cccggtagaa ggcgaccgtc ttctccatgt cgttcgtgat
     3181 catcgcgacg tggtgcagca tcgcgaatgg cgctttcagt ccgtcgcggt ccggtagcga
     3241 cagcggtgtc gtcgttgtca ttggaagtcc tctcctggca accagagtcc gatcgtgtgg
     3301 cggtcgtcag acgatcgtac cccgaaaacc atgccctgcg acagtttccg cacctcctgc
     3361 cggcggggta tgacagagat gacacgtatt cgagcgccgc aacaggagat cgcgaccgtg
     3421 aatgattctg aaccgatgga ggcaccgacg agcgggttcg acgccgagcg cgtcgccgct
     3481 gctcaccatc gcaataccct ccagcgcaac gtccagaacc ggatcatgcg cgagaaggcg
     3541 cgcctcctgc acgactggac aaccgctggc ggctccgaag acgacttcga ggaagtctgg
     3601 ccctccatcc gcgcccaact cggccagatc cgcgtcatgg agataggtga caaggcccgc
     3661 gcccgctcgc ttactgcgtt taatcggagg tgactacggt aggtgatagg tgttaggtgt
     3721 taggggttgg tcggtgagga ttgatgtgac gcgttcttcc ttcacgaaac aacccctaac
     3781 ccctaacacc taccgcagtc ccgacatcac cggtcggaac atgcgcgtcg cgacgcccga
     3841 gatgatcaac gccccaccgg cgaccgcgaa cgcgattggc acactggata gttctgcaat
     3901 cgcacccagc atcaggaagc cgatcggcat caacccgttg atcatcgact gcgccgccac
     3961 ggctcgcccg cggaagcggt cctcggcaaa ggtctggacc agcgtgtcgt tctgaatgat
     4021 gtagaccatc tggaaatagc cggtcacgaa cagccccggc atgatgacgc ttgcgctgct
     4081 cccggccgcg acgatcagca gtcccgcccc gaacccgatc gtcgcgatcc agatcgtccg
     4141 gccccgcagt tcgcgccccg tcagcgcccc ggccgtcagc gtcccaaaca tcgcgccgac
     4201 gccactgacg gcgaacagca gccccagcat cgtcgcgccg ccgtcgagcg tgtcgctggc
     4261 gatggccggc agcagcgcga tgtgcgccat cccgaacagg tacggcacga tgtcgttcac
     4321 cagcgccgag cggagcagcg gattcgtcct gatatagccg accgcatcgc caatgtcact
     4381 gcggaacgag ggccgggcgg caggttgtag cgggccgcgc gtggccgaga ctccctcggc
     4441 actcagcagc agcggcacca gcagcaccag cccggccgcc ggcaggtaga tccccgacgc
     4501 cagcagcagc gccgccgcga ccccggcgct gtcgacgatc aaccccgaca ccgtcggcgc
     4561 cgccacccgc gacaggttcc ccatcatcga gttgatcgcg ttggcgctct gcagcgtccg
     4621 gaccggcacg aggctgggca gcagcgacga gtgagccggg tacgagaacg cgttggcgat
     4681 ccccatcagc acgaccgtta ccg
//
LOCUS       DASUGJ010000444         5135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1737538, whole genome shotgun sequence.
ACCESSION   DASUGJ010000444 DASUGJ010000000
VERSION     DASUGJ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737538"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFV93_09775"
     CDS             complement(<1..630)
                     /locus_tag="VFV93_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEX5165672.1"
                     /translation="MATSQIGDAINEIGERVLSRLEAINAARERALAETRQIVRMSAN
                     AIRAVHRDDYDEAARLVEQARVMQDSLAAVLAEHPNIYWSGYVQDAQKEYAEANIVLG
                     IISGRGLPSPEDLSVENAVYLNGLGEAAGELRRYVLDALRRSQPARAEPLLAAMDDIY
                     GLLVTVDYPDAITGGLRRTTDMVRGVLERTRGDLTVALQNRSLLEALERA"
     gene            930..1748
                     /locus_tag="VFV93_09780"
     CDS             930..1748
                     /locus_tag="VFV93_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5165673.1"
                     /translation="MKTMLNMVELPTQVRMPYVEQGDPWGTPVVLLHGYPDSWFSFTP
                     LLPHLPESIRAIVPTQRGWGDAGRPAEGYTMDDYAGDVLALLDALRIEAAVIAGHSMG
                     SLIAQRFAIRHPERTLGLVLIGSVTTFAGHAGLLEFQQAIEALDGPLDPDLVREFQES
                     MFVQPPEPELLEGLIAESMKAPLPVWKQSLAGILAFDTRLELSRISAPTRIIWGDQDQ
                     LCSRAEQDALLAGISNARLSVYRGGGHCVNWEQPAAVAAEIVDLAARVSDPVGC"
     gene            1954..2613
                     /locus_tag="VFV93_09785"
     CDS             1954..2613
                     /locus_tag="VFV93_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165674.1"
                     /translation="MHTTTLALRRPLILLVVVVTAMTTLLAANVSADQAEIGTPEEDC
                     VAHVADDCRGQPGNGTLTVVEFDISEDMTRFSFDADTTHEDGLPARGSAFITQGYLYP
                     AGTLNGSNGVLPDGSPEFPELVLGEWTCYGYYVGDGAHTTTGAMVITTQVFNIGDGIG
                     GETIVTSGYEVADLNVAVARAITGGTGTYRNARGETSQQMLGLNATEGVVLRVTLNVQ
                     K"
     gene            2788..3762
                     /locus_tag="VFV93_09790"
     CDS             2788..3762
                     /locus_tag="VFV93_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEX5165675.1"
                     /translation="MELGGLHHVTAVTGNAAGNLDFYTRVLGLRLVKKTVNQDDVSAY
                     HLFYADGKGNAGTEVTFFDWPDAGPAVHGSGTIARIGLRVPGRDSLDWWQARFNELGV
                     THDAIEEHGGRSMLPFEDPEGQRFALVADTGGGMEQGVVWEQSTVPAERQIRGLNHVL
                     LSVQQLAPTALVLTEVLGFRQADSYPSPENPSRQIVVFETGPGGFGTEVHVEEMPDQR
                     YGRVGIGGVHHIAFRTPTDEEHRQWRDRVRSFRIGITDVIDRYYFRSLYFREPGHILY
                     EIATDGPGFAVDEDASTLGESLVLPPFLEPRRAEIVAGLKPLDTSRVA"
     gene            3912..>5135
                     /locus_tag="VFV93_09795"
     CDS             3912..>5135
                     /locus_tag="VFV93_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5165676.1"
                     /translation="MATRTLRPHLSHWGAFDAEVEDGALVAIRPFEHDPDPSPLLGNI
                     PASLRHPTRVTQPMVREGWLVNGPGPSERRGAEPFVPVDWDTATELLAGELKRVYAEH
                     GAPAIYGGSYGWASAGRFHHAQSQLHRFLNCLGGYTSSVGAYSHAAGSTILPHVIAPA
                     PTFMFTGTHWRILAEQTDLFVAFGGMPLKNTNVTSGGVFRHRTRDFLQQAAERGAEFV
                     LVGPQRDDLATFVNADWYPISPGTDVALMLALAHTLIVEGLHDRAFLDRYCVGFDRLE
                     RYILGSDDGRPKSAEWAAELTEVAAGDIRALARRMAGKRVFITTTWSLQRAEFGEQPP
                     WMAVALAAILGQIGLPGGGYGFGYGSSQRVGEGAALPGLKLPTFGQGRNPVDSFIPVA
                     RIADMLHNPGAPFDYD"
BASE COUNT          856 a         1761 c         1666 g          852 t
ORIGIN      
        1 ggcccgttcg agggcttcca gcaggctgcg gttctggagt gcaaccgtca gatcgccccg
       61 tgtccgttcc agcacaccac gcaccatatc ggtcgtccgg cgtagcccgc cggtgatcgc
      121 gtccgggtag tcgaccgtca ccagcagacc gtagatgtcg tccatcgcgg ccagcagcgg
      181 ctcggcccgc gctggctggg agcgccgcag ggcatccagc acatagcggc gcagttcgcc
      241 ggccgcctcg ccgagcccgt tcaggtagac ggcgttctcg acgctcagat cctccggcga
      301 cggcagccca cggccggaga tgatcccgag gacgatgttg gcctcggcgt actccttctg
      361 cgcgtcctga acgtagccgg accagtagat gttcgggtgc tcggccagga ctgctgccag
      421 cgagtcctgc atcacccgcg cttgctcgac cagccgggcg gcctcgtcgt agtcgtcgcg
      481 gtggacggcg cggatcgcgt tggcgctcat ccgcacgatc tgccgtgtct cggccagcgc
      541 ccgctcgcgg gcagcgttga tcgcctccag ccgcgacagc acccgctcgc cgatctcatt
      601 gatcgcgtca ccgatctggc tcgttgccat tcagccatcc tctctgccct gcccgtctac
      661 cggccgcacg ttccattgtg acaccatcgc gccgtccgtg gcggatgcgc tcgcatgaga
      721 agaatcactc attggcccaa ctggccggtg gtgctggctc tgggtagttt cggcaggggc
      781 agcggagcga tcgccgcatc acccactcca ccggcgctcc atgaatggtc aattgcgtcg
      841 atggcggacc atatcgcgac ccctagagta cggcgatgac cgcgacattg gggtcgcggt
      901 cggggcaaac gccaaaggag gcactgccaa tgaagaccat gctcaacatg gtcgaacttc
      961 ccacgcaggt ccggatgcca tacgtcgagc agggcgaccc gtggggtaca cccgtcgtcc
     1021 tgctgcacgg ctacccggat tcctggttct cgttcacgcc gttgttgccg catctgccgg
     1081 aatcgatccg ggcgatcgtg ccgacccagc gcggctgggg cgatgccggc cggcctgctg
     1141 aggggtacac gatggacgac tacgccggtg acgtcctggc cctgctggat gccctgcgga
     1201 ttgaggccgc cgtcatcgcc ggtcactcga tgggtagcct gatcgcccag cgatttgcga
     1261 tccgccaccc cgagcgcaca ctcgggctgg tcctgatcgg cagcgtcacg accttcgccg
     1321 gccacgccgg cctgctggag ttccagcagg cgatcgaggc gctggatggc ccgctcgatc
     1381 ccgatctcgt gcgcgagttt caggagagca tgtttgtcca gccgccggag cctgagttgc
     1441 tcgaagggtt gatcgccgag agcatgaagg ccccactgcc cgtctggaag cagtcgctcg
     1501 ccggcatcct cgcgttcgac actcgactcg agctctcgag gatcagcgca ccgacacgca
     1561 tcatctgggg cgatcaggac caactctgct cacgcgccga acaagacgcc ctgctggccg
     1621 gaatcagcaa cgcgcgcctc tccgtctatc gcggcggcgg ccattgcgtc aactgggagc
     1681 aaccagccgc ggtcgcggcc gagatcgtcg atctcgcagc ccgcgtcagc gaccccgtcg
     1741 gatgctgatg ccatccgtca tccagacatc gccgtctggc cattcccaaa ggagggacaa
     1801 cgatgttctc gattcatctc cagcaactcc tggtgcgaca ggaacgcgcc aggatcgaac
     1861 gcctcagccg agacgcctgg aagcgtcacg aaggccggca ctagccggtc gatccataac
     1921 gaccaaccaa tgcctgtgaa aggacagata acgatgcaca ccacgactct cgcactccgc
     1981 cgcccgttga tcctgctggt ggtcgtcgtc accgccatga cgacgctgct agccgccaac
     2041 gtctcagccg accaggctga gatcgggacg cccgaagagg actgcgtcgc ccatgtcgcc
     2101 gatgactgtc gaggacagcc gggcaacggc acgctaaccg tcgttgaatt cgacatctcc
     2161 gaggacatga cccgcttcag cttcgacgcg gacacgactc acgaggacgg cctgccggct
     2221 cgcggcagcg ccttcatcac acagggatat ctgtaccccg ccggcacgct gaacggcagc
     2281 aacggcgtcc tgccggacgg ctcgccagag ttcccggaac tggtgcttgg cgagtggacc
     2341 tgctacggtt actacgtcgg tgacggcgcc cacacaacga cgggagcgat ggtcatcacg
     2401 acccaggtct tcaatatcgg tgacggaatt ggcggggaga cgatcgtcac gtccggctac
     2461 gaggttgccg acctgaatgt cgcggtcgcg cgtgccatca ccggcggaac cggcacttac
     2521 aggaacgcgc gcggcgagac cagccagcag atgctcggcc tcaacgcgac tgagggcgtc
     2581 gttcttcggg tgacactgaa cgtccagaag taaccacgcc gcaccgcatc aacagacgta
     2641 atacccggcc gccggcgacg gtagccgggt gttacgcgtt gtggcacggt tgcgcgtgga
     2701 atgcgagttg ctgtcatttg ttatgacatg ttgagggaat cgcgcggagc gcgatcgcgc
     2761 cccgtgtcag caacaaggga gtaacagatg gaactgggtg ggctgcatca cgtcacggct
     2821 gtcaccggca acgcagccgg caacctcgat ttctacaccc gcgtgctcgg cctgcggctg
     2881 gtcaagaaga cggtcaacca ggacgacgtc tccgcctacc acctcttcta cgcggacgga
     2941 aagggcaacg ccggcaccga agtgaccttc ttcgactggc ccgacgccgg cccggccgtc
     3001 cacggcagcg gcacgatcgc ccggatcggc ctgcgtgtgc cgggccgcga cagcctcgac
     3061 tggtggcagg cacgcttcaa cgagctgggc gtcacccatg acgcgattga ggagcacggc
     3121 gggcggtcaa tgctgccgtt cgaggatccg gaggggcagc gcttcgccct cgtcgccgac
     3181 acgggcggcg ggatggagca gggggttgtc tgggagcaga gcacggtccc ggccgagcgg
     3241 cagattcgcg gcctcaacca cgtcctgctc tcggtccagc aactggcgcc gacggcactc
     3301 gtcctgaccg aagtgctcgg cttccgccag gccgacagct acccgtcgcc ggagaatccc
     3361 tcgcggcaga tcgtcgtctt cgagaccggc ccgggcggct tcgggaccga ggtgcacgtc
     3421 gaagagatgc ccgaccagcg ctacggccgg gtcgggatcg gcggcgtcca ccacatcgcc
     3481 ttccggacgc caaccgacga ggagcatcgc cagtggcgcg accgcgtccg ctcgtttcgg
     3541 atcggcatca ctgacgtgat cgaccgctat tacttccgct cgctctactt ccgcgaaccg
     3601 ggccacatcc tctacgagat cgcgaccgac ggccccggct tcgccgtcga tgaggacgcg
     3661 tcaaccctcg gcgagagcct cgtcctgccg ccattcctcg aaccccgccg ggccgagatc
     3721 gtcgccgggt tgaagccgct ggacacgtcg cgggtggcgt gaggcggggg tcgggattcg
     3781 ggacgcggga cgcgggatgt tcccagacgt gcgatgctcc cgaggaccgc atcccgaatc
     3841 ccggttcccg aatcccgccc aaaccagtac cattcccgcc tgactcgttg aacgactcgg
     3901 aggcgtgctg aatggcgaca cggaccctgc ggccacattt gtctcactgg ggcgcgtttg
     3961 acgccgaggt tgaggatggg gcgctggtcg cgatcaggcc gttcgagcac gatcctgacc
     4021 cttcgccact gctggggaat atcccggcca gcctgcggca tccgacacgg gtgacgcagc
     4081 ctatggtccg cgagggctgg ctggtaaatg ggccagggcc gagcgagcgg cgcggagccg
     4141 agccgttcgt gccggtcgat tgggatacgg cgactgagct gctggccggt gagctgaagc
     4201 gggtctacgc cgagcatggc gcgccggcca tctacggcgg ctcctacggc tgggccagcg
     4261 cgggacggtt ccaccacgcc cagagccagc tacaccgctt cctcaactgc ctcggcggct
     4321 atacctcgtc ggtcggcgcc tacagccacg cggccggcag cacgatcctg ccgcacgtca
     4381 tcgctccagc cccgaccttc atgttcaccg gcacccactg gcggatactg gccgagcaga
     4441 ccgacctgtt cgtcgccttc ggcgggatgc cactcaagaa cacgaatgtc acctccggcg
     4501 gcgtcttccg gcatcggacg cgcgacttcc tccagcaggc ggccgagcgg ggcgccgagt
     4561 tcgtactggt cggcccgcag cgcgatgacc tggcaacgtt cgtcaacgcc gactggtatc
     4621 cgatctcgcc ggggaccgat gtcgcgctga tgctggcgct ggcccatacg ctgatcgtcg
     4681 agggtttgca cgatcgcgcg ttcctcgatc gctactgcgt cggcttcgac cggctggagc
     4741 gctacatcct cggcagcgac gatgggcggc caaagagcgc cgagtgggcg gcggagctga
     4801 ccgaggttgc ggctggcgac attcgcgccc tcgcccggcg gatggccggt aagcgcgtct
     4861 tcatcacgac gacctggtcg ctccagcggg ccgagttcgg cgagcagccg ccctggatgg
     4921 cggtcgcgct ggcagcgatc ctcggccaga tcgggctgcc gggcggcggc tacggcttcg
     4981 gctacggttc ctcgcagcgg gttggcgagg gtgccgctct gccgggactc aagctgccaa
     5041 ccttcggtca gggccggaat ccggttgact cgttcattcc ggtcgcgcgg atcgccgaca
     5101 tgctgcataa ccccggcgcg ccgttcgact acgac
//
LOCUS       DASUGJ010000445         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_624766, whole genome shotgun sequence.
ACCESSION   DASUGJ010000445 DASUGJ010000000
VERSION     DASUGJ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624766"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..926
                     /locus_tag="VFV93_09800"
     CDS             135..926
                     /locus_tag="VFV93_09800"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX5165677.1"
                     /translation="MRLYVEVARRAFARYLTYRSANVAGLITNGFFGVLISAVFIAAY
                     AGRGVEAGWTEEDALTFVWLAQALLMPVHVFAWWEIALTIRSGDVVSDLSKPFDYYAF
                     WLSHDYGRALYHTLFRGIPTLTLGWLLFDIRLPNRPEVWLAFLISVGLAIWISFGLRF
                     LANVAAFWLLDYRGAGMALLFFNAFLSGQLVPLVYWPETVREIVVWLPFAGLVQTPVE
                     VFLGKASGVDLAGLLLLQVSWGIVLMGACRLLLALAVRRVVIQGG"
     gene            929..1756
                     /locus_tag="VFV93_09805"
     CDS             929..1756
                     /locus_tag="VFV93_09805"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX5165678.1"
                     /translation="MRNWLPDDLRSDLRLYRRLVGSRLRSQMQYRASFMTMMIVTGFG
                     LFAELIATAILLNRFGDLAGWGIGEVALLYGLASVSFGLGELAGAGFDAFPATIRRGE
                     FDHVLLRPAGAFMQVLSADFQLRRLGRIGQGIVALGLAFSWTSIDWTPLKMLFLGVTL
                     ISGMVMFIALFVFGAALCFWTVESIEVINSVTDGGNVLASYPLPIYHELMQRVFTFII
                     PLAFVSYIPAVYLLDRPEANDWPSWLPLLSPLAALLLALAARVAWGVGVRHYRSTGS"
     gene            1836..>2085
                     /locus_tag="VFV93_09810"
     CDS             1836..>2085
                     /locus_tag="VFV93_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5165679.1"
                     /translation="MIDVTHLSKRFWVARHHRGVGGLLRNLVSRDGQEVRAVDDISFS
                     VADGEMVGYVGPNGAGKSTTIKMLTGILVPSSGTLRVLD"
BASE COUNT          265 a          655 c          710 g          455 t
ORIGIN      
        1 ccaattggtt cattgcgttg agggcgagtc accgacgtcg cccctacgca ttgattgcct
       61 gattgatgtg aatcccgcgt cccgccagat ctgctataat cagcgcccct ttcgtcatga
      121 tggcaggagg atgggtgcgg ctgtatgtcg aggtcgcgag gcgggcgttc gcgcgctatc
      181 tcacctaccg atctgccaat gtcgccgggc tgatcacgaa tggctttttc ggtgtcctga
      241 tctcggcagt cttcattgcc gcctacgcgg ggcgcggggt ggaggccggc tggaccgagg
      301 aggatgcgct gaccttcgtc tggctcgccc aggcgctgtt gatgccagtc catgtcttcg
      361 cctggtggga gatcgcgctg acgatccgca gcggagacgt cgtctccgac ctgagcaagc
      421 cgttcgacta ctacgccttc tggctgagcc atgactacgg tcgcgcgctc taccacacgc
      481 tctttcgtgg gattccgacg ctgacgctcg gctggttgct cttcgacatc cgcttgccca
      541 atcggccgga ggtgtggctg gcgttcctga tcagcgtcgg gctggcgatc tggatcagct
      601 tcgggttgcg cttcctggcg aacgtcgcgg ccttctggtt gctcgactac cgaggggcgg
      661 ggatggcgct gctgttcttc aatgcgtttc tctccggcca gctcgtgccg ctcgtctact
      721 ggccggaaac ggtgcgcgag atcgtcgtct ggctgccgtt cgcggggttg gttcagacgc
      781 cggtcgaggt ctttctcggc aaggcgagcg gggtggatct cgccggcctc ctcttgttgc
      841 aggtgagttg gggaatcgta ctgatgggcg cctgccgctt gctgctggcg ctggcggtcc
      901 gacgcgtcgt catccaggga ggctgagggt gaggaactgg ctgccggacg acctgcgatc
      961 cgacctgcgc ctctaccggc ggctcgtcgg atcacggctg cgctcgcaga tgcagtaccg
     1021 cgcctcgttc atgacgatga tgatcgtgac cgggttcggt ctcttcgccg agttgatcgc
     1081 gaccgcgatc ctgctcaacc gcttcggcga cctggccggc tgggggattg gcgaggtcgc
     1141 gctgctgtac gggctggcct cggtctcgtt cgggctgggc gagctggccg gcgccgggtt
     1201 cgacgccttt ccggcgacga ttcggcgtgg tgagttcgac cacgtgctgc tgcgcccggc
     1261 cggcgcgttc atgcaggtgc tctcggccga cttccagctt cggcggctgg gccggatcgg
     1321 ccagggcatc gttgcgctcg ggctggcctt ctcgtggacg tcgatcgact ggaccccgct
     1381 gaagatgctc ttcctcggcg tcacgctcat ctcggggatg gtgatgttca tcgccctgtt
     1441 cgtctttggc gcagctctct gcttctggac ggtcgagagc atcgaggtca tcaactcggt
     1501 gacggacggc ggcaacgtcc tcgccagcta cccgctgccg atctaccatg agttgatgca
     1561 gcgcgtcttc accttcatca tcccgctggc gttcgtctcg tacatcccgg ccgtctacct
     1621 gctcgatcgc cccgaggcca acgactggcc cagctggctg ccgctgctct cgccgctggc
     1681 ggcgcttctg ctcgcgctgg cggcgcgcgt cgcctggggc gttggcgtgc ggcattatcg
     1741 gagtacgggg agttagcgtt cgggacgtgg gaggtgggac gcgggacgcg attgaatcta
     1801 accccgcgtc ccacgtcccg acccacggag gcacgatgat cgacgtcact cacctcagca
     1861 agcggttctg ggtcgcgcgg catcatcgcg gggttggtgg gttgttgcgc aacctggtct
     1921 cgcgtgatgg gcaggaggtg cgggccgtcg atgacatctc gttctcggtc gcggacggcg
     1981 agatggtcgg ctacgtcgga ccgaacggcg ccggcaagtc cacgacgatc aagatgctga
     2041 ccgggatcct cgtcccgtcg tccggcaccc tgcgcgtcct cgacc
//
LOCUS       DASUGJ010000446         6171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_68885, whole genome shotgun sequence.
ACCESSION   DASUGJ010000446 DASUGJ010000000
VERSION     DASUGJ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68885"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            602..2377
                     /gene="glmS"
                     /locus_tag="VFV93_09815"
     CDS             602..2377
                     /gene="glmS"
                     /locus_tag="VFV93_09815"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX5165680.1"
                     /translation="MCGIFGYVGAEADTGEMVLSALRRLEYRGYDSWGVGVGCDGAIA
                     IEKQVGKIGTAVVDLPAAEIGFGHTRWATHGGVTQANAHPMSDATGRLAVIHNGIIEN
                     HRQLRERLITRGYRFQSETDTEVVVHLVADRLEGGRSAGDVLCAVSDVFKELRGLNAI
                     ILLDQETRTLTAVKNVSPLVIGRGSTGLYVASDTLALAGHAGEVMYLDDLEVAQLGPD
                     GVRVVSTTTGERVAERWVPIPVDLGAADLGGYDHFLQKEIFEQPNVIERIAREQIDGV
                     RELAAMIRESYGTFLVGCGTASYAALTGNYLFSRIARRHVNFVVGSEFAYQEHFLTDR
                     SLVVAHSQSGETVDVIEPVLAAKARGAKIAALVNVKGSTLDRIADFSLHLNAGPEQAV
                     LSTKAYSAKIATLLLTAHILNGSEHVGRDLLWRAVDGVRQTLTPTFVDRVREVAACIN
                     DQTSMFVVGRGLSYPTALEAALKIKEVSYIHAEGFAGGELKHGVIALIEQGTPCIVYS
                     PLDETRDDIISGAMEMKARGGYIIGISPEAEDVFDIHLPIADAGDASPLVMAPPAQML
                     GYQLAVLRGLDPDKPRNLAKSVTVK"
     gene            2374..3180
                     /locus_tag="VFV93_09820"
     CDS             2374..3180
                     /locus_tag="VFV93_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5165681.1"
                     /translation="MISLIDSHAHIDLPVYDDDREAMLGRALAAGVHAIVVIGHNRER
                     WRTSAALCARVPWLVRSVGLHPNDAADWSDALRRDLEAQLEAGGPVAIGETGLDFYRD
                     SAPEDLQRRAFAAQIEFAQRYELPIIIRQRSAEQPVLEMLREHGPVRGVMHCFSGDSS
                     FARECVEAGLMLGVGGIATYPKSQDVRDALATVPIESLVLETDAPYLAPHGWRGKRNE
                     PGHVVVAAEVLAAVHGMTLDAVAKATTVNAIGLFGASLEAARLAGMAAAK"
     gene            3177..3821
                     /gene="rdgB"
                     /locus_tag="VFV93_09825"
     CDS             3177..3821
                     /gene="rdgB"
                     /locus_tag="VFV93_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX5165682.1"
                     /translation="MKVIVATNNRGKLEELRALLPDGLDLLTLSDAGLDSPEESGTTF
                     EENALIKARAAAPHADAAFADDSGLVVDALDGRPGVWSSRFAGPAATDADNNARLLAE
                     LSDVSDDDRTARFVSVVALVTSDGHEFIATGCVEGRIGRQERGAGGFGYDPLMIVADP
                     NAVEFIGLTMAELSVEQKNLISHRARAYRELLSVLREAGFSFGMADDSSGVTEG"
     gene            3829..5082
                     /locus_tag="VFV93_09830"
     CDS             3829..5082
                     /locus_tag="VFV93_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165683.1"
                     /translation="MAMLSMQTGVPWRVQAAGHNGQVLPIVAIAGTRGKSTVSWMLSD
                     ILRSAKMPAASWLSSGVYVEGERQEGELGPWSRVVLAARHGEIDVAIQEMNAATVVGA
                     GLPADTYPLVVLTTLCGNNESCLLNAETELQRRALDVVVRAARKDGSIVANADDYDIA
                     ELAQGAGVDGMLYALHRDNPALQRHLGEGGRGAWVDDGWIVVGTSAGADPIVSVNSIP
                     ATLDGTILFQVQNALAAVCASKVIGIPVKAISEALHQFEPHPDLQPAACNIVRFNQAT
                     ILIDAPRQVMSLRLLARGIKHKPHRRTLVVSGSFSGLGDTDAHEAGRILGSLGGIILL
                     HNENTDPARMDAIKSGISSSPVPPIVMAVPGELRAIDHLLNTIAPDDVALVIADDADA
                     VLRRLWPAPVISVEGTRKAGMVSHT"
     gene            5079..5762
                     /locus_tag="VFV93_09835"
     CDS             5079..5762
                     /locus_tag="VFV93_09835"
                     /inference="COORDINATES: protein motif:HMM:NF004981.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HEX5165684.1"
                     /translation="MSDISQVVHDFHTHTYLSDGVLSPMEQARRAVVNGYQTLGLTDH
                     MGVGGVAQLLETLRRDREIIERHWPIQVIVGVELTHVPAEAIHEAAAHARSEGAEIIV
                     VHGETPVEPVPDGTNHASIACGLVDVLAHPGFLTEADAYLAAQNDVFLEITARRGHSL
                     TNGHVARLAREAGARMIVNSDAHEPSDLLKPAFQRAVALGAGIAESLLDEILVGNPTQ
                     LLDRVLSRR"
     gene            complement(5918..>6171)
                     /locus_tag="VFV93_09840"
     CDS             complement(5918..>6171)
                     /locus_tag="VFV93_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165685.1"
                     /translation="TLRSSGDAGEDRKRLERLVPLFRDHFAGDDLLYIQLEESGRRRL
                     LRSSFRIDWCEALDAAISEVVGPNACAAISSRSLTTSVG"
BASE COUNT         1094 a         1902 c         2051 g         1124 t
ORIGIN      
        1 ggtgtatcga tcgatcacga tggctcccta tccacgttcc aggcaaacag gtacgtcgac
       61 gctagtagca cgcgccgaaa tgagaatgcc gcctcgcggc ggcggttggc tcgtggttgc
      121 ggggtatgga cgcgcgcccg gcaggcgcag gctggtgact gaaacagact gtacgttcgc
      181 gatgtttgtg atttcatcga cccttgtcat cgctcgcaaa ctgtcgcgcc gaagtataca
      241 agattgaaag gttcgctgcc gccggcccct cttgcgggtt gccgagcgat ggcggtatag
      301 tggcaccgag ccgcgacagg tgggcggacg acgtttcgca ttgatgtagc gccgggactc
      361 gtgtgggagc gcgagttgac gaggtgaagg ccgtatcgaa ccattcggcg gatggccttc
      421 cggttggcac gaccgagacg atttcgacaa agcggctggg tgaccggccg ggacaaaagg
      481 aatcgagtgc gtttcctacc tgaccgcttc ggtcaattcc accttcgcgt tgctccagtt
      541 tgatcacgac agacatgacc cggtgacggc gttgggcctc ccgggctgaa ggaacgcgcg
      601 catgtgtgga atattcggct acgtcggcgc tgaggccgac acaggcgaga tggtgctgtc
      661 tgccctgcgg cggctcgagt accgcggcta cgattcctgg ggcgtcggcg tcggctgcga
      721 cggcgcgatt gcgatcgaga agcaggtcgg caagatcggc acagctgtcg tcgatctgcc
      781 ggccgccgag atcggcttcg gccacacccg ctgggcaacc catggtggcg tcacccaggc
      841 caacgcgcac ccgatgtcgg acgctacggg gcggctggca gtgatccaca acggcatcat
      901 cgagaatcac cgccagcttc gcgagcgact gatcacccgg ggctaccgct tccagagcga
      961 gactgacact gaggtcgtcg ttcacctcgt cgccgaccgg ctggagggtg gacgatcggc
     1021 tggcgacgtg ctctgcgcag tctcggacgt cttcaaggag ctgcgcggcc tgaacgcgat
     1081 catcctgctc gatcaggaga cacgcaccct gacggccgtc aagaacgtct cgccgctggt
     1141 gatcggtcga ggctcgaccg ggctgtatgt cgcgtccgac acgctggcac tggcgggtca
     1201 cgccggcgag gttatgtacc tggacgacct cgaggtcgcc cagcttgggc ccgatggagt
     1261 gcgcgtcgtt tcaacgacga ccggcgagcg ggtcgctgaa cgctgggttc cgatcccggt
     1321 cgatctcggc gcggccgatc tcggcggcta cgaccacttc ctccagaagg agatcttcga
     1381 gcagcccaac gtgatcgagc gcatcgcccg cgagcagatc gacggcgtcc gcgagctggc
     1441 ggcgatgatc cgcgagtcgt atgggacatt cctggttggt tgcggcaccg ccagctacgc
     1501 cgccctgacc ggcaactacc tcttctcgcg catcgcccgc cggcacgtca acttcgtcgt
     1561 cggcagcgag tttgcctacc aggagcactt cctgaccgac cgctcgctgg ttgtcgccca
     1621 ctcacaatca ggcgagacgg tcgacgtgat cgagccggtc ctggccgcca aggcgcgcgg
     1681 cgccaagata gcggcgctcg tcaacgtcaa gggatcgacg ctggatagga tcgctgactt
     1741 ttcgctacac ctcaacgccg ggccggagca ggcggtgctc tcaaccaagg cgtacagtgc
     1801 caagatcgcg accctgctgc tgacggccca tatcctgaac ggcagtgagc acgtggggcg
     1861 agatctgctc tggcgggcgg ttgacggcgt tcgtcagaca ttgacgccga cattcgtcga
     1921 ccgagtgcgc gaggttgccg cttgcatcaa tgaccagaca agtatgttcg tcgtcggccg
     1981 tggcctgagc tacccgacgg cgctggaggc agcactcaag atcaaggaag tctcgtacat
     2041 ccacgccgaa gggttcgccg gcggtgagct gaagcacggt gtcatcgcgc tgatcgaaca
     2101 gggtacgccc tgcatcgtct actcgccgct ggacgagaca cgcgacgaca tcatctccgg
     2161 cgcaatggag atgaaggccc gcggtggata catcatcggc atctcgccgg aagctgagga
     2221 tgtgtttgac atccacctgc cgatcgcgga tgctggcgat gcctcgccac tggtcatggc
     2281 cccgccggct cagatgctcg gctaccagtt ggcggtcctg cgcggcctcg atccggataa
     2341 gccgcgcaac ctcgcgaaga gtgtgacggt gaagtgattt cactaatcga cagccacgcc
     2401 cacatcgacc tcccagtcta cgacgacgac cgggaggcga tgcttggccg ggcactggct
     2461 gctggtgtcc acgcgatcgt cgtgatcggc cacaaccgcg agcggtggcg gaccagcgcg
     2521 gccctctgcg ctcgcgtccc gtggctggtt cgctcggttg gtcttcatcc aaatgatgcc
     2581 gctgactggt cagacgcgct ccggcgagac ctggaagcgc aactggaagc cggtgggccg
     2641 gttgcgatcg gcgagaccgg gcttgacttc taccgcgact cggcgccgga agaccttcag
     2701 cggcgggcct tcgcggccca gatcgagttt gcgcagcgct acgaattgcc aatcatcatc
     2761 cgccagcgca gcgccgagca gccagtgctc gagatgttgc gcgaacacgg gcccgttcgc
     2821 ggcgtcatgc actgcttcag cggtgactcg tcgttcgccc gggagtgtgt cgaggccggg
     2881 ctgatgctcg gcgtcggcgg tatcgcgacc tatccgaagt cgcaggacgt tcgcgacgcg
     2941 ctggcgaccg tcccgatcga gagcctcgtt ctggagacgg acgcaccata cctcgcgcca
     3001 cacggctggc ggggcaagcg caacgagccg ggtcatgttg tcgttgcggc agaggtgctg
     3061 gccgccgtcc atgggatgac gttggacgcc gtggcgaaag caacgacggt gaacgccatc
     3121 ggtctgttcg gagcatcgct cgaagcggcg cggctggccg ggatggcggc ggccaaatga
     3181 aggtgatcgt cgcgacgaac aatcggggca agctggagga gctccgcgcg ctgctgcccg
     3241 atggtctgga cttgctgacg ctcagtgacg ccggcctcga ctcgccggaa gagtccggta
     3301 cgacgttcga ggagaacgca ctgatcaagg ccagagcagc cgcgccgcac gccgatgcgg
     3361 cattcgcgga tgactctggc ctggtcgtcg acgccctcga tggccggccg ggggtctggt
     3421 cctcacgctt cgctgggccg gcggcaacgg acgcagacaa caatgccagg ctgctggcag
     3481 agttgagcga tgtctccgat gatgaccgga cggcgcgctt cgtctcggtc gtcgcgctgg
     3541 tgacgagtga tgggcatgag ttcattgcca ctggctgcgt cgagggacgg attggcaggc
     3601 aggaacgtgg cgcgggtggt ttcggctacg acccgttgat gatcgtcgcc gatccgaacg
     3661 cggtagagtt tattggtttg acgatggcgg aattgtcggt agaacagaag aacctgatat
     3721 cgcaccgtgc acgcgcctac cgcgagctgc tgtcggtgct gcgtgaggca ggtttcagtt
     3781 ttgggatggc ggacgattcg tccggtgtta ccgaggggta gttacagcgt ggcaatgctg
     3841 tcgatgcaga caggggtgcc gtggcgcgtg caggcggctg gtcacaacgg tcaggtcctg
     3901 ccgatcgtcg cgattgcggg aacacggggc aagtcgacgg tcagttggat gctctcggac
     3961 atccttcggt cggcgaagat gccggcggcg agttggctct catccggcgt gtatgtcgag
     4021 ggcgagcgcc aggagggtga gctgggtccg tggtcgcgtg tcgtcctggc ggcgcgacac
     4081 ggcgagatcg acgtcgcgat tcaggagatg aacgccgcaa ccgttgtcgg cgccggcttg
     4141 ccggccgaca cctacccgct cgtcgtcttg acgacgctgt gtggcaacaa cgagtcctgc
     4201 ctgctcaacg ccgagaccga actgcaacgg cgggcactcg atgtcgtcgt tcgcgccgcg
     4261 cgcaaggatg gctcgatcgt cgccaacgcc gacgactatg acatcgccga actggcacag
     4321 ggcgccggcg tcgatggcat gctttacgca ctccatcgcg acaatccggc gctgcaacgg
     4381 cacctcggcg agggtgggcg gggcgcctgg gtcgacgatg gctggatcgt cgttggcacc
     4441 agcgccggtg cggatccgat cgtatccgtc aactcgattc ccgctacgtt ggacggaacg
     4501 atcctgtttc aggttcagaa cgcgctggcg gccgtctgcg cgtcgaaggt catcggaatt
     4561 ccggtcaagg cgatctcgga ggcgctccat cagtttgagc ctcacccgga tcttcagccg
     4621 gcggcctgca acatcgtccg tttcaaccag gcaacgatcc tgatcgacgc tccgcggcag
     4681 gtgatgtcct tacggttgct ggcgcgcggg atcaagcaca agccgcatcg caggacgctg
     4741 gtggtcagcg gttcattctc cggcctcggt gataccgacg cccacgaagc cggtcggatt
     4801 ctggggagcc tgggtgggat tatcctgctg cacaacgaga acacggaccc cgctcggatg
     4861 gacgcgatca agagcgggat ctcgtcgtcg cccgtcccgc cgatcgtgat ggccgtaccc
     4921 ggcgagcttc gggcgatcga tcacctgttg aacacgatcg caccggatga cgtggcgctc
     4981 gtcatcgccg acgatgctga cgctgtcttg cgtcgtctct ggccggcacc ggtcatcagt
     5041 gttgaaggga cgcgcaaggc agggatggtg agccacacat gagtgacata tcccaggtcg
     5101 tccatgactt tcacacccac acctacctat ccgacggcgt actgtctcct atggaacagg
     5161 cacgtcgcgc agtcgtaaat ggctatcaaa cgctgggact taccgaccac atgggtgtcg
     5221 gcggcgtcgc acagttgctg gagacgttgc ggcgtgaccg ggagattatc gagcggcact
     5281 ggccaattca ggtcatcgtt ggcgtcgagc tgacacacgt cccggccgag gcgattcacg
     5341 aggcagctgc ccacgcgcgc tccgaagggg cagagatcat cgtcgtccac ggcgagactc
     5401 ccgtcgagcc ggtgccggat gggacgaatc acgcctcgat cgcctgcggg ctggtcgatg
     5461 tcctcgccca ccctggcttt ctgacagaag ccgacgcgta cctggcggcc cagaacgatg
     5521 tctttctcga gatcaccgcc cggcgcggac attcgttgac gaacggccac gtcgcccggc
     5581 tggcgcgcga ggctggtgcc cgcatgatcg tcaacagcga cgcgcacgag ccatcggacc
     5641 tgctgaaacc agcctttcag cgcgccgttg cgcttggcgc cggcatcgcc gagtcgctac
     5701 tggacgagat actggtcggc aatccaacgc aactgctgga tcgcgtgctc tcccgccgct
     5761 gattctcatc ggaaatccat ctccactcga accgaccaat gaacggtcga tgggcagctg
     5821 tcattgtgca gtgtcgaggt attttcaaac taatgggata tgtaacctag actcggtcgc
     5881 aatgcttctt ctgcgcgtcc accaatcagt aggtttgcta tccgacggag gttgtcagcg
     5941 accgactgct gatagccgcg caagcgttgg gaccgacgac ctcgctgatg gccgcatcga
     6001 gcgcttcgca ccagtcgatc cggaacgatg agcgcaggag tcgtcgacgg ccgctctcct
     6061 ccagttggat gtagagcaag tcatcaccgg cgaagtggtc gcggaaaagc ggtacaagcc
     6121 gctcgagacg cttgcgatcc tcgccggcat caccggacga ccgcagcgtg a
//
LOCUS       DASUGJ010000447         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1875436, whole genome shotgun sequence.
ACCESSION   DASUGJ010000447 DASUGJ010000000
VERSION     DASUGJ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875436"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VFV93_09845"
     CDS             <1..1181
                     /locus_tag="VFV93_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX5165686.1"
                     /translation="EEGNGMATGVNGRHGFSTRAIHAGEGSDPTTGAHNTPIYQTSTY
                     AFHSLEQKAAVMSGEAEGYVYTRGGNPTTSAFEQKVAALEGAEAATAGGSGMAVIMAA
                     MRSFVRSGDHIVASDDVYHWATIYLEEEAQLDRIDVTRVDIGDLDAVRAALRPETKVI
                     YTEFLSNPQIKVADIAGLAEIARAAGVLLIVDNTFASPYLLRPLEHGADLVLHSATKY
                     LCGHGDALAGVIAGRRELIEPIHRQIQLLGSCISPFNSWLLLRGIKTLEMRMERHCRN
                     ALELARFLDRQPDVEVVLYAGLESHPGHDVAARLMEHGFGGMLSFRLRGRTEAANQFA
                     NALQLCDHAVSLGDVNTLVWPHINDLVRVSVGCENAEDIIADFEQALEVSSAAVGVTA
                     "
     gene            complement(1256..>2073)
                     /locus_tag="VFV93_09850"
     CDS             complement(1256..>2073)
                     /locus_tag="VFV93_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HEX5165687.1"
                     /translation="AVPTCMKGIQRMNRRDVPKAALIDVDGVLHIDGDPIPGAVEALA
                     HLRELDVPFRLLTNTTVRTRAALGNLLRGLGFDVADEEIITAPVAAAAYVRRRFPDQP
                     CYAIVKDAVLGDFGGITLTDGPEARVVIVGGAEENFTYQAMNHAFRLLMDGAALIAIH
                     RNLWWMTAVGPTLDAGAFVKGLEHVTGRRATVVGKPAAPFFRAGFRSLGLPPSQVVMV
                     GDTHRHDILPAIRLGATGALVQTGSFRESDLVAGAPDYLLASVAELPNLFTAQ"
BASE COUNT          371 a          665 c          723 g          314 t
ORIGIN      
        1 acgaggaggg gaacgggatg gcaacggggg tcaacggcag gcacgggttc tcgacgcggg
       61 cgattcatgc cggggagggg agcgacccga cgaccggggc gcacaacacg ccgatctatc
      121 agacatcgac ctacgccttc cattcgctgg agcagaaggc agccgtcatg agcggtgagg
      181 ccgaggggta tgtctacacc cgtggcggca acccgacgac gagcgccttc gagcagaagg
      241 tggcggcact ggagggggcc gaagcagcca cggccggcgg ctcggggatg gccgtcatta
      301 tggcggcgat gcgctcgttc gtgcgttccg gcgaccacat cgtcgcctcc gacgacgtct
      361 accattgggc gacaatctac cttgaggagg aagcccagct cgaccggatc gacgtcacgc
      421 gggtcgacat cggcgatctg gacgccgtcc gggcggcgct gcggccggag acgaaggtga
      481 tctacaccga gttcctctcc aacccgcaga tcaaggtcgc cgacatcgcc gggctggccg
      541 agatcgcccg ggccgccgga gtgctgctga tcgtcgacaa caccttcgcc agcccgtatc
      601 tgctgcggcc actggagcac ggcgcggacc tggtcctgca ttcggcgacg aagtacctct
      661 gcggccacgg cgacgcgctg gcgggcgtca tcgccggtcg ccgcgagctg atcgagccga
      721 tccaccgcca gattcaactg ctcggctcgt gcatcagccc gttcaactcg tggctgctgc
      781 tgcgcgggat caagacactg gagatgcgga tggagcgcca ttgccgcaac gcgctggaac
      841 tggcgcgctt cctcgatcgc cagccggatg tcgaggtcgt cctctacgcc gggctggaga
      901 gccaccccgg ccacgatgtc gcggcccggc tgatggagca tggcttcggt gggatgctct
      961 cgttccggct acggggcagg actgaagccg ccaaccagtt cgccaacgcg ctccagctct
     1021 gcgaccacgc cgtcagcctc ggcgacgtca acacgctcgt ctggccgcac atcaacgacc
     1081 tggtccgcgt ctcggtcggc tgcgagaacg ccgaagacat catcgccgac ttcgagcagg
     1141 cgctggaagt gtcgagcgcg gcggtggggg tgacggcgta gccgccagcc ctttcccaat
     1201 catcaatcat caatccggta cgggcgatat cgcgatatcg tccgtaccgg attggctatt
     1261 gagcagtgaa gagattgggc aattccgcga ccgacgccag caggtaatcc ggcgcgccag
     1321 caacaagatc ggattcgcgg aaggagccgg tctggacgag ggcaccggtc gcgccgaggc
     1381 gaatggcggg caggatgtcg tggcggtggg tgtcgccgac cataacgacc tgcgacggcg
     1441 gcaggccgag cgagcggaac ccggcacgga agaatggggc ggccggcttg ccgacgacgg
     1501 tcgcgcggcg gccggtgacg tgttcgaggc ccttgacgaa ggcgccggcg tcgagggttg
     1561 ggccgacggc cgtcatccac cagaggttgc ggtgaatcgc gatcagggcg gcgccgtcca
     1621 tcagcagccg gaaggcgtga ttcatcgcct ggtaggtgaa gttctcctcg gcgccgccga
     1681 cgatgacaac gcgggcctcc gggccgtcgg tcagcgtgat gccgccaaag tcgccgagga
     1741 cggcgtcctt gacgatcgcg tagcagggct gatccgggaa acgcctgcgt acgtacgcgg
     1801 cagccgccac cggcgccgtg atgatctcct cgtcagcgac gtcgaagcca agcccgcgca
     1861 gcaggttgcc cagcgcggcg cgggtccgaa cggtcgtgtt ggtcagcagc cggaagggaa
     1921 cgtccagctc gcgcaggtgc gccagcgcct caaccgcacc cggaatcggg tcaccgtcga
     1981 tatggagaac accgtcgaca tcgatcaggg cggctttggg tacatcacgt cgattcatcc
     2041 gctgaatgcc cttcatgcag gttggaaccg cat
//
LOCUS       DASUGJ010000448         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1181413, whole genome shotgun sequence.
ACCESSION   DASUGJ010000448 DASUGJ010000000
VERSION     DASUGJ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181413"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..>2101)
                     /locus_tag="VFV93_09855"
     CDS             complement(44..>2101)
                     /locus_tag="VFV93_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEX5165688.1"
                     /translation="QHHGTAILVLGALFNALKVTNRTLEDSSVVIAGAGIAGIGVARL
                     LHRAGVRRLVVCDRAGAIYSGRPERMNWAKAYLAKETNRERRRGSLAEMLKGADVFIG
                     LSSEGTATPEMIETMAPDPIVLALAIPTPEITPQLARDAGAKVVATGRSDYPNMMDIS
                     LVFPGVFRGLLDSGARNMRLRTLIYAAKALASLVPDEELNPDNIVPRIFDFRVAPTIA
                     SAVVQAALETGEAQNDVRPEAVAERTRRYVYEGIMPPLAHANGGPGPRKKPETLRDEV
                     LTLRRRHGGVLEIRSKIPIKDHHILNILYVPPAALAPAVEIRNDPMSVYDLTVKDNLV
                     AIVTDGTAVLGLGDIGPEAALPVMEGKAVLFQTLAGVEAFPICIAERDPDKIVDIVAN
                     ISPSFGGINLEDISAPRCFDIEAKLKERLDMPVFHDDQHGTAVVVTAALLNALRLRGS
                     SLENTQVAINGAGAAGIAVAKLLLSSGIGDIIMCDRAGAIHKGRKTSMNRSKEEIARL
                     TNREGVKGALCDAVVGADVFIGLSAPGALTGDDIRRMNDKPIIFALANPTPEILPEAA
                     HAAGALVIATGRSDFPNQVNNSLAFPGIFRGALDAKARDINDAMKVAAAQAIADLVSE
                     EELAADYVVPEALDLRVPPAVAAAVARAAVETGVNRVAIDPADVEQRTRDRIYTGQPA
                     NWE"
BASE COUNT          317 a          703 c          715 g          366 t
ORIGIN      
        1 ggtcagagcc acccagcact cagcacccag catccagcac tcgttactcc caattcgccg
       61 gctggccggt gtagatgcgg tcgcgggtgc gttgttcgac gtcggcgggg tcgatcgcga
      121 cgcggttgac gccggtttcg acggcggcgc gggcgacggc ggcagcgacg gcaggtggaa
      181 cacgcaggtc gagcgcttcc ggcacgacgt agtcggcggc cagctcctct tcgctgacca
      241 gatcggcgat ggcctgggcg gcagccacct tcatcgcgtc gttgatgtcg cgcgccttgg
      301 cgtcgagtgc gccccggaag atgccgggga atgccagcga gttgttgacc tggttgggga
      361 agtccgaccg gccggtcgcg atgaccagcg cgccggctgc gtgggcggcc tcgggcagga
      421 tctcaggcgt cgggttcgcc agcgcgaaga tgatcggttt gtcgttcatc ctgcggatgt
      481 catcgccggt cagcgcaccg ggcgcggaga gaccgatgaa gacgtccgcg ccgacgaccg
      541 cgtcgcagag cgcgcccttg acgccctcac ggttcgtcag ccgggcgatc tcctccttcg
      601 agcggttcat actcgttttg cgccctttgt gaatcgcccc agcccggtcg cacatgatga
      661 tgtcgccgat gccggacgat agcagcagct tggcgacggc aatcccggct gcgccggcgc
      721 cgttgatcgc gacctgagtg ttctccaggc tcgacccgcg cagccgcagc gcattcagca
      781 gcgccgccgt cacaacaacc gccgtcccat gctggtcgtc gtggaagacc ggcatgtcca
      841 gccgctcttt gagcttcgct tcgatgtcga agcagcgcgg cgcggagatg tcctccaggt
      901 tgatgccgcc aaacgacggc gagatgttgg cgacgatgtc gacaatcttg tcggggtcgc
      961 gctcggcgat gcagatcggg aacgcctcga caccggccag cgtctgaaag aggacagcct
     1021 tcccctccat caccggcaaa gccgcctccg ggccgatatc gcccaggccg aggacggccg
     1081 tgccgtcggt cacgatcgca accaggttgt ccttgacggt caggtcgtag acgctcatcg
     1141 ggtcgttacg gatctcgacg gccggggcca gtgcggccgg cggcacgtag aggatgttga
     1201 ggatgtggtg atccttgatc gggatcttcg aacgaatctc caggacgccg ccgtgccgtc
     1261 gccggagtgt cagcacctcg tcgcgtaagg tctccggctt cttgcgcggg ccaggaccgc
     1321 cgttcgcgtg cgccagcggc ggcatgattc cctcgtagac gtagcgccgg gttcgctcgg
     1381 caaccgcctc cggccggacg tcgttctgcg cctcgccggt ctccagtgcc gcctggacga
     1441 cggccgaagc aatcgtcggc gcgacccgga agtcgaagat ccgcgggacg atgttgtcag
     1501 ggttgagctc ttcgtcgggg acgagtgagg ccagcgcctt cgcggcatag atcagcgtcc
     1561 gcagccgcat gttgcgcgcg ccactgtcca gcagcccccg gaagacgccc gggaagacca
     1621 gcgagatgtc catcatgttc gggtagtcag agcggccggt cgcgacgacc ttcgccccgg
     1681 catcgcgggc cagttgcggc gtgatctcgg gtgtcgggat cgccagcgcc aggacgattg
     1741 ggtccggcgc catcgtctcg atcatctccg gcgtggccgt gccctcgctc gacaggccga
     1801 taaagacatc ggcgcccttg agcatctctg ccagcgagcc gcgccgtcgt tcgcggttgg
     1861 tttccttggc cagataggcc ttggcccagt tcattcgctc cggccggccg ctgtagatcg
     1921 ctccggcccg gtcgcagacg accagccggc ggacgccggc ccggtggagc aggcgggcga
     1981 ccccaatccc ggcgatgccc gcgccggcga tgacgacgct actgtcctcc agcgtccggt
     2041 tcgtcacttt caacgcgttg aacagcgccc cgaggacgag aatcgccgtt ccgtggtgct
     2101 g
//
LOCUS       DASUGJ010000449         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1738526, whole genome shotgun sequence.
ACCESSION   DASUGJ010000449 DASUGJ010000000
VERSION     DASUGJ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738526"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /gene="zapE"
                     /locus_tag="VFV93_09860"
     CDS             <1..514
                     /gene="zapE"
                     /locus_tag="VFV93_09860"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HEX5165689.1"
                     /translation="QREIGALSASFESIRVDGEDYRHRQFDHEEDLPRILPAEVIARD
                     WDAVAWPALTARLADLPPLAYPALIASFETLAIAGIDSLPDQDTALRFVHFIDKAYDR
                     SLSLVASTDCRLDELFPPSYGYGGFERKFLRCRSRLYEMLSQPLPRERGVELDSFDNG
                     LETIEHAHRT"
     gene            498..878
                     /locus_tag="VFV93_09865"
     CDS             498..878
                     /locus_tag="VFV93_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5165690.1"
                     /translation="MLIARDADRDVDLWRDGVETRVYASATAGSRQLVVFEQVCAPGV
                     GAPAHLHAVEEVLRVIEGEAEVSVGDERATLGPGDAVTIPAGIVHGFTNSGAAPLRVL
                     AILASPTFEAHYVDPPRDSRRWLP"
     gene            complement(891..1181)
                     /locus_tag="VFV93_09870"
     CDS             complement(891..1181)
                     /locus_tag="VFV93_09870"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5165691.1"
                     /translation="MKTIEMPTATDEQLVEMLRAVANPVRYRILQILAERGECQCGPL
                     GDLLPIAPSTLSQHLKVLREAGLISGTVDGPTICYCREEQGIAWLKDHIASL"
     gene            complement(1303..>2137)
                     /gene="arsM"
                     /locus_tag="VFV93_09875"
     CDS             complement(1303..>2137)
                     /gene="arsM"
                     /locus_tag="VFV93_09875"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX5165692.1"
                     /translation="MTQQQEPAASAIRDVVRERYASAVTAVLNRESASCCGGSAAEAS
                     CCGSGSVVSPIVIGNYTPGETSSLPEEAVLASFGCGNPVALAKLEPGQTVLDLGSGGG
                     IDVLLSAKRVGPTGFVYGLDMTDEMLELAERNKQAAGIDNVRFLKGEIEAIPLPEAAV
                     DVIISNCVINLAADKDVVLADAFRVLRPGGWFAVSDVVVQGDLTDELRSSMDAWAGCV
                     AGALEEHDYLRRLTAAGFVDASIEVTSTYDLADIGCCADAANTTNGSVVSAFIRARKP
                     E"
BASE COUNT          377 a          721 c          659 g          380 t
ORIGIN      
        1 ccagcgcgag atcggggcgc tgtcggcgtc gttcgagtcg atccgggtcg atggcgagga
       61 ctatcgccat cgccagttcg accatgagga agatctgcca cgcatcctgc cggcggaggt
      121 catcgctcgc gactgggacg ccgtcgcctg gccggcgctg actgcgcggc tggcggacct
      181 gccgccgctt gcctatccgg cgttgatcgc gtcgttcgag acactggcga tcgccggaat
      241 cgacagcctg cccgatcagg acactgccct gcgcttcgtc cacttcatcg acaaggccta
      301 cgaccgctcg ctctcgctgg tcgcatcaac cgattgccgg ctggacgagc tcttcccgcc
      361 cagctacggc tatggcggct tcgagcgcaa gttcctgcgc tgccgttctc gcctgtatga
      421 gatgctctca caaccgctgc cgcgtgagcg gggcgtcgaa ctggacagct tcgacaacgg
      481 actggagacg atcgagcatg ctcatcgcac gtgatgcaga ccgagacgtc gatctctggc
      541 gcgacggcgt cgaaacgcgg gtctacgcgt cagccacggc aggcagccgg caactggtcg
      601 tcttcgagca ggtctgcgcg ccaggcgtcg gcgcacccgc ccacctgcac gccgtcgagg
      661 aggtattgcg agtgatcgag ggcgaggcag aggtcagcgt cggcgacgaa cgggcgacgc
      721 tcggccctgg cgatgctgtc accattccag cagggatcgt ccacggcttc accaacagcg
      781 gcgcggctcc gctacgagtg ctggcgatcc tcgcctcgcc aaccttcgag gcccactacg
      841 tcgatccacc gcgcgactcg cgccgctggc tcccctgacc acacaggatg tcaaaggctc
      901 gcgatgtgat ccttgagcca ggcgatgccc tgctcttcgc ggcagtagca gatggttggc
      961 ccgtcaacag tgccggagat cagacccgcc tcacgtagca ctttgagatg ctgggagagc
     1021 gtcgagggcg cgatcgggag cagatccccc agcgggccgc actggcactc tccgcgctcg
     1081 gccagaatct gcaggatgcg gtaacggaca gggttggcaa ccgcgcgcag catctccacc
     1141 agttgctcgt cggtcgccgt cggcatctca atcgtcttca tcagtctgtc agccagtcag
     1201 ttcgtcaacc ggtcagcccg tcagtcgggt tgcgttcgtc gcgtccgatc atagcacctc
     1261 ggtcatcgtc gactctcgat ggatatctgt cgagccagtc cactactcag gcttacgggc
     1321 gcggatgaat gcgctgacaa cgctgccgtt ggtggtgttg gcagcgtccg cgcagcagcc
     1381 gatgtcagcc aggtcgtagg tgctggtcac ctcaatgctg gcgtcaacaa agccggcggc
     1441 ggtgagacgg cgcaggtaat cgtgctcttc aagcgcaccc gcgacgcaac cagcccaggc
     1501 gtccatactg gagcgcaact catccgtcag atcaccctgg acgacgacgt ccgagacggc
     1561 aaaccagcca cccggtcgca ggacacggaa cgcatcggcg aggacgacat ccttgtcggc
     1621 agcaaggttg atgacgcagt tcgagatgat cacgtcaacc gcagcctcag gcagcgggat
     1681 tgcctcgatt tcgcccttga ggaaacggac gttgtcgatc ccggctgcct gtttgttgcg
     1741 ctcggccagt tccagcatct cgtcggtcat gtcgagtccg tagacgaagc ccgtcgggcc
     1801 gacccgcttg gccgacagca acacgtcgat cccgccgccc gagccgagat cgagcaccgt
     1861 ctggcccggt tccagcttcg ccagcgcgac cggattgcca caaccaaatg acgccagcac
     1921 cgcctcctcg gggaggcttg acgtttcgcc cggcgtgtaa ttgccaatca cgattggcga
     1981 aacaacgctg ccgctaccgc agcaactcgc ttcagctgcc gacccgccac aacatgatgc
     2041 cgactcccgg ttcaggacgg ctgtgacggc gctcgcatac cgctcacgaa ctacgtcgcg
     2101 aattgcgctg gctgcgggtt cctgctgctg tgtcatc
//
LOCUS       DASUGJ010000450         3135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1600214, whole genome shotgun sequence.
ACCESSION   DASUGJ010000450 DASUGJ010000000
VERSION     DASUGJ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600214"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..606)
                     /locus_tag="VFV93_09880"
     CDS             complement(67..606)
                     /locus_tag="VFV93_09880"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5165693.1"
                     /translation="MQIETERLRLREFRGDDWPAMLAYWNDPRYQRYYPDLPNREQVV
                     RELVGLFVAAQAEQPRQKWQLAVARKDGGRLIGNCGIRVNNPDQGEANIGYELDPRDW
                     GNGYATEAATAIVRFGFEELGLHRIWAECVADNTASAHVLEKLGLRREAHFREHQYYR
                     NRWWDTYIYAILSHEWRDQ"
     gene            681..2933
                     /locus_tag="VFV93_09885"
     CDS             681..2933
                     /locus_tag="VFV93_09885"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5165694.1"
                     /translation="MREPEHPEMVCPRCGLWNPQRNATCESCGERLHQPIEPAEPPAA
                     TPQASSAAAVAAAFPAALPGLGALPGVSVPPAEEPRLVQTAPRPRPDEPSLYAPVIGP
                     ADRESFFDAQRRNRRATWRLTAGSAVAAIVTGIPLSLIVTPVLFAVILILTRLIHIVV
                     PVPDTIWDAYRSSGLILGDVIDAMDDPSTPGVDEGDLGRVPISLFLKAAGVWLLPGIL
                     LMLLIWPLLIRLFRHGGVGGVLLSIGARPPVMTDLEERQLVNIVEEMAIAAGLTPPRV
                     MLLDSGIANAAAIGSSPADATIVVSRALLDDLDRDETQGILGHLVASIGNGDLRAALS
                     IVAIFQTFGFASALVKAPLSRQARKTVWRMLTYIFNPRKPQERAAEARVVSEMLTTGI
                     WDTDDDSELMDIDDDQPAPVERPGPSLRLLLYFPIFFIAVFVATLLLGFPDPLRRLTM
                     IGVVGLALLLIWYQREYAVHQFVFGAKVTRAIIILPYYMGAMMPQFMLMLMTSFMLEP
                     LLALLWRTRRYLADATAVQLTRQPDGLAQGLTGLVQRGGVIPGGKWAGPLFVVGPEAM
                     SKRMTDAIQAQYKQHLDASLAEGDTDGKRGFMEEARAMMEAQRKMAREAQQAQQNTGQ
                     PVEPSMIALATSYAEASGATQESGFTASGDSGVVSFHPPLAKRLKKLRKMGAHVADAD
                     LIARPMFRRPDSIRSPLGLLLAPVVFALMAIVAILMVVVIVLMGILALGACAILMAVV
                     YGLFLLIAPT"
BASE COUNT          484 a         1066 c         1019 g          566 t
ORIGIN      
        1 ataggggcga ttgtgtgatt gacattgagg gcgagtcacc gacatcgccc ctacgcattg
       61 atgtatttat tgatcgcgcc attcgtggct caggatggcg tagatgtagg tgtcccacca
      121 gcggttgcgg tagtactggt gttcgcggaa gtgggcttcg cggcgcaggc cgagcttttc
      181 gaggacgtgg gctgaggcgg tgttgtcggc cacgcattcg gcccagatgc ggtgcaggcc
      241 gagttcctcg aagccgaagc ggacgatcgc cgtcgccgcc tcggtcgcgt aaccgttgcc
      301 ccagtcgcgc gggtccagct cgtagccgat gttcgcctcg ccctgatctg ggttgttgac
      361 acggatgccg cagttgccga tcagccggcc gccgtccttg cgcgcgacgg cgagttgcca
      421 tttctggcgg ggctgctccg cctgggccgc gacgaacagc ccgaccagct cgcggacgac
      481 ctgctcccga ttcggcagat cggggtagta gcgctggtag cgcggatcgt tccaataggc
      541 cagcatggcc ggccagtcgt cgccgcgaaa ctcacgcagt ctcagccgtt ccgtctcgat
      601 ctgcatcgtt gctccccgcg ctcgcaccgg cgtccgtgac aggaccgatg aatgcgctac
      661 actgtaatcg atacccgatc atgcgagaac cggagcatcc ggagatggtc tgtcccaggt
      721 gcgggttatg gaatccccag cgcaatgcga cctgtgagtc gtgcggggaa cggctgcacc
      781 agccgattga gcctgccgag ccaccagcag ccacaccaca ggcgagctcc gctgccgctg
      841 ttgccgccgc gttcccggcc gcattgccgg gtctcggcgc gctgcccggc gttagcgtcc
      901 cgcctgccga agaaccccgt ctggtccaga ccgcaccgcg tccccggccg gatgaaccgt
      961 cactctacgc gccggtcatc gggccggccg atcgcgagag cttcttcgat gcccagcgca
     1021 ggaaccgacg ggcaacctgg cggctgacgg ccggctcggc tgtcgccgcg atcgtgaccg
     1081 gcatcccact ctcgctgatc gtcacgccgg tcctcttcgc cgtcatcctg attctgacgc
     1141 ggctgatcca catcgttgtc ccggttccag acaccatctg ggacgcctat cgcagcagcg
     1201 ggctgatcct cggtgacgtc atcgacgcga tggacgaccc atcgacgccg ggcgtcgatg
     1261 agggggatct cggccgggtg ccgatctcgc tctttctgaa ggcggccggg gtctggctgc
     1321 tgccggggat cctgctgatg ctgctgatct ggccgctgct gatccggctc ttccggcatg
     1381 gcggcgtcgg cggcgtcctg ctctcgatcg gcgcccggcc tccagtcatg accgacctcg
     1441 aagagcggca actggtcaac atcgtcgagg agatggcgat cgcggccggc ctgacgccgc
     1501 cgcgtgtcat gctgctcgat tcggggatcg ccaacgcggc ggccattggc tcttcaccgg
     1561 cggacgccac gatcgtcgtc tcccgcgcac tgctggacga cctcgaccgc gacgagacgc
     1621 agggcatcct cggccacctg gtcgcctcga tcggcaatgg cgacctgcgg gcggcgctgt
     1681 cgattgtcgc gatcttccag acgttcggct tcgcctcggc gctcgtcaag gcgccgctct
     1741 cccgccaggc ccgcaagacc gtctggcgga tgctcaccta catcttcaac ccccgcaagc
     1801 cgcaggagcg ggctgccgag gcgcgcgtcg tctccgagat gctgacgacc ggcatctggg
     1861 acacggacga cgacagcgaa ctgatggaca tcgacgacga tcaacccgcg ccggtcgaga
     1921 ggccggggcc atcgctcagg ctgctgctgt acttcccgat cttcttcatc gccgtcttcg
     1981 tcgcgacgct gctgctcggc ttccccgacc cgctgcgccg gctgacaatg atcggcgtcg
     2041 ttgggctagc gctcctgctc atctggtacc agcgcgagta cgccgtccac cagttcgtct
     2101 tcggcgcgaa agtcacgcgc gcgatcatca tcctgcccta ctacatgggc gcgatgatgc
     2161 cgcagttcat gctgatgctg atgacgagct tcatgctgga gccgctgctg gcgctgctct
     2221 ggcgcacccg ccgctacctg gccgacgcga ccgccgtcca gctcacccgc cagccggacg
     2281 gcctcgcgca ggggctgacc ggcctggtgc agcggggtgg ggtcatcccc ggcggcaagt
     2341 gggccggtcc gctcttcgtc gtcggcccgg aggcgatgag caagcggatg accgacgcca
     2401 ttcaggcgca gtacaagcag cacctcgacg cgtcactcgc cgagggcgat acggacggca
     2461 agcgcggctt catggaggag gctcgcgcga tgatggaggc ccagcgcaag atggcgcgcg
     2521 aggcccagca agcccagcaa aacaccggcc agccggtcga gccatccatg atcgcgctcg
     2581 ccacctccta cgccgaggcg agcggggcca ctcaggaatc gggcttcacc gccagcggcg
     2641 acagcggcgt cgtcagcttc cacccaccgc tggcgaagcg gttgaagaag ctgcggaaga
     2701 tgggcgccca tgtcgccgac gccgacctga tcgcccggcc gatgttcagg cgcccggaca
     2761 gcatccgatc tccgctggga ctgctgctgg cgccggtcgt gttcgcgctg atggcgatcg
     2821 tcgccatcct gatggtcgtc gtcatcgtcc tgatgggtat cctcgcactc ggcgcctgcg
     2881 ctattctgat ggcggtggtc tatggcctgt tcctgctcat tgcgccgacg tagaagagcg
     2941 tgacgcgtga cgcgtgacgc gatgaggctc ggagacatcc atcggtcgag acgaactccg
     3001 cgtcacgcgt cacgcattac gcgtcacgca tcaccgaaag gaaccaacca tgtgggtacc
     3061 tatcgcgatc ggtgtcgtta ttctgctgct ggcgttgttc atgatcctga cctacaacgg
     3121 gctggtcggg aagcg
//
LOCUS       DASUGJ010000451         3308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1459027, whole genome shotgun sequence.
ACCESSION   DASUGJ010000451 DASUGJ010000000
VERSION     DASUGJ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459027"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2360)
                     /gene="lon"
                     /locus_tag="VFV93_09890"
     CDS             complement(<1..2360)
                     /gene="lon"
                     /locus_tag="VFV93_09890"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5165695.1"
                     /translation="MPRRSQKTTETSDALIDAEDLGDAYVAAPERVKPDDESTYLTLP
                     TLILPQTLLLPRMSLPYPLDDEEESQAVDDAIKHDRLILVLAAKDQDDVDEADLDDDE
                     DDESDFIPPDESRAGVGSYGSLVADDELCTVGVIAEVGHKITRPGGPAHVILQGVARG
                     VVKDLEQRRPFTLAKVKRVDDANVESLEAKAVMSHVLEQVEAYVAMLPNVPEEVVTMV
                     RSVDEPGWLADLIAFSPEFESAKRQEALEILNPVERLRHISVILQERLEYLTLRHQIQ
                     SEAQAGLDRQQREYLLREQMRTIQRELGDIAGEEALVQEFRDKIEAAGMPDEVKAKAL
                     LQVSRLEHQHPFSPEIGVIRSYLEWLTELPWSIETEDQLDLKDAARILDEDHYGLQKV
                     KERILEFIAVRKLAGNRMKAPIICFVGPPGVGKTSLGKSIARALGRSYVRMSLGGVRD
                     EAEVRGHRRTYVGAMPGRVIKALRDAKTKNPVMVLDEIDKVGSDAFRGDPSSALLEVL
                     DPEQNHSFSDHYLEVAFDLSQVIFITTANLLDPIPAPLRDRMEIIEVPGYTEVEKLEI
                     ARNFLLPKALYSHGLEASQLMVEDDALLRLIREYTHESGVRNLDREIGALCRKVARRV
                     AEEPERESWSVEADDLPGFLGIPRFEYGLAEENDEIGVATGAAVTSAGGDLLSVETTV
                     MAGKSDLTLTGQLGDVMQESARAAISYARSRSEELGIPTGFFDSHTIHIHVPAGAIPK
                     DGPSAGVTLATSLISAVTGSRVRKDVAMTGEVTLRGKVLPIGGVRE"
     gene            complement(2511..>3308)
                     /locus_tag="VFV93_09895"
     CDS             complement(2511..>3308)
                     /locus_tag="VFV93_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5165696.1"
                     /translation="SGWRLGRGFPGAELLSLDSLTHRYGQTIALRDISLTGHAGEVIA
                     LIGRNGSGKTTLLKHVNGLLRPGSGRVLHRGQDIARTPVHEAARVAGYVPQHPTSILH
                     QETLGDELRFTARAQRKQIAPEPLLEQLGLLARIDQHPLDLSGGERQRAALAAIAIGA
                     PDILLLDEPTRGLPGNDKRMLAGFVRDYARQGRLVIVATHDVEFVASAADRVVMLAEG
                     EIVADGSPAEVLSGSLTFGTQMNRLFGGDVLTVEDALRLMRTMNAER"
BASE COUNT          522 a         1117 c         1096 g          573 t
ORIGIN      
        1 ttctcgcgga cgccgccgat cgggaggacc ttgccacgca gcgtcacctc gccggtcatc
       61 gcgacatcct tgcggacgcg gctgccggtc acggctgaga tcagcgaggt cgccagcgtc
      121 acgccggccg aggggccgtc cttcgggatc gcgccggccg ggacgtggat gtggatcgtg
      181 tggctgtcga agaagccggt cgggatgccc agttcctctg aacgcgagcg ggcgtaggag
      241 atggctgccc gcgccgactc ctgcatgaca tcgccgagct gaccggtcag tgtcaggtcc
      301 gacttgccgg ccatcacggt cgtctcgacc gagagcaggt cgccgccggc gctcgtcacg
      361 gccgcgccgg tcgcgacgcc gatctcgtcg ttctcctcgg ccagcccata ctcgaagcgc
      421 gggataccga ggaagcccgg cagatcgtcc gcttcaaccg accagctctc gcgctccggt
      481 tcctcggcga cgcggcgtgc taccttccgg cagagcgcgc cgatctcgcg atcgaggtta
      541 cggacgcccg actcgtgcgt gtactcgcgg atcaagcgca gcagcgcgtc gtcctcgacc
      601 atcaactggg acgcttcgag accgtgtgag tagagcgcct tcggcagcag gaagttgcgg
      661 gcgatctcca gcttctcgac ctcggtatag cccggcacct cgatgatctc catccggtcg
      721 cgtagcggcg ccgggatcgg gtcgagcagg ttggcggtcg tgatgaagat cacctgcgag
      781 aggtcgaagg cgacctccag gtagtgatcg gagaaggagt ggttctgttc cgggtcgagc
      841 acttccagca gcgccgacga cggatccccc cggaaggcgt cggagccaac cttgtcgatc
      901 tcgtccagga ccatgaccgg gttcttcgtc ttcgcgtcgc gcagcgcctt gatgacgcgg
      961 cccggcatcg caccgacgta ggtgcggcgg tggccgcgca cctcggcctc gtcgcgaacg
     1021 ccaccgagcg acatccggac atacgagcgg cccagcgcgc gggcgattga cttgccgagg
     1081 ctcgtcttgc cgacgcccgg cggaccaacg aagcagatga tcggcgcctt catccggttg
     1141 cccgccagct tgcggacggc gatgaattcg agaatgcgct ccttgacctt ctgcaggccg
     1201 tagtggtcct catcgagaat ccgggcggca tccttcaggt cgagctggtc ctcggtctcg
     1261 atcgaccagg gcagctcggt cagccactcc aggtaggaac ggatgacgcc gatctccggc
     1321 gagaatgggt gctggtgctc cagccgcgag acctgcagga gcgccttggc cttgacctcg
     1381 tccggcattc cggccgcttc gatcttgtcg cggaactcct ggaccagcgc ctcttcgccg
     1441 gcgatgtcgc caagctcgcg ctggatcgtc cgcatctgct cgcgcagcag gtactcgcgc
     1501 tgctggcggt cgagcccggc ctgcgcctcg gactggatct ggtgccgcag cgtcaggtac
     1561 tccagccgct cctgaaggat gacggagatg tgccgcagcc gctcaaccgg gttgaggatc
     1621 tccagcgcct cctggcgctt ggccgattca aactccggcg agaaggcgat caggtcggcc
     1681 agccagcccg gctcgtcgac gctgcgcacc atcgtcacga cttcttctgg cacgttcggc
     1741 agcatcgcga cgtaggcctc gacctgttcg agaacgtgcg acatgacggc cttcgcctca
     1801 agcgactcga cgttggcgtc gtcaacgcgc ttgaccttgg ccagcgtgaa tggccggcgc
     1861 tgctccagat ccttgacgac accccgcgcg acaccctgga ggatgacatg cgccgggccg
     1921 ccaggccggg tgatcttgtg acctacttcg gcgatgacac cgaccgtgca gagttcgtcg
     1981 tcggcgacca gcgagccgta gctgccaacg ccggcgcgcg actcgtcggg cgggatgaaa
     2041 tcgctctcat cgtcctcgtc gtcatcgagg tcggcctcgt caacgtcgtc ctggtccttg
     2101 gcggccagga cgaggatcag ccggtcgtgc ttgatcgcgt cgtcaaccgc ctgggactct
     2161 tcctcgtcgt caagcgggta gggcagcgac atgcgcggca gcagcagcgt ctgcggcagg
     2221 atcagtgtcg gcaacgtcag ataggtcgat tcgtcgtccg gcttgacccg ctccggcgcg
     2281 gcgacatatg cgtcgccgag gtcctccgca tcgatgagtg cgtcgctggt ctcggttgtc
     2341 ttctgggagc gtcggggcat gcaatgtgtc ctcaagtgaa aagcgcggaa ccgttgcaga
     2401 tcaggtcctc atgctcccgc ccggcgcagg tttgcgacgc tgaagatgag cgcgccagtc
     2461 aacgggcatg gccgacaatc ttaccaatcc gcagcacgat gcgtcagtga tcaacgctcg
     2521 gcgttcatgg ttcgcatgag gcgcagcgca tcctcaactg tcaatacgtc gccaccgaag
     2581 agccggttca tctgcgtgcc gaaggtgagc gagccggaga ggacctcggc cgggctgccg
     2641 tcggcgacga tctcgccctc ggccagcatg acgacgcggt cggcggcgct ggcgacgaac
     2701 tcgacatcgt gggtggcgac gatgacgagc cggccctgtc tggcgtagtc gcggacgaag
     2761 ccggccagca tccgcttgtc gttgccgggc aggccgcgcg tcggctcatc cagcagcagg
     2821 atgtccggcg cgccgatcgc gatcgctgcc agtgctgccc gctgccgctc gccgccgctc
     2881 aggtcaagtg ggtgctggtc gatccgcgcc agcaagccaa gttgttccag caacggctcc
     2941 ggcgcgatct gcttgcgctg ggcgcgggcc gtgaagcgca actcgtcgcc caacgtctcc
     3001 tgatggagga tcgaggtcgg gtgctgcggc acgtaccccg ccacccgcgc cgcctcatgg
     3061 acgggcgtgc gcgcgatgtc ctgcccgcga tgcaacaccc gcccgctacc cgggcgcagc
     3121 agcccgttga cgtgcttgag cagcgtcgtc ttgcccgacc cattccgccc gatcagcgcg
     3181 atcacctcac cagcgtggcc ggtcaggctg atgtcacgca gggcgatggt ttggccgtag
     3241 cggtgggtga gggagtcgag gctaagcaac tcagcgcccg gaaaacccct ccccaaccgc
     3301 cacccaga
//
LOCUS       DASUGJ010000452         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1459344, whole genome shotgun sequence.
ACCESSION   DASUGJ010000452 DASUGJ010000000
VERSION     DASUGJ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459344"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="VFV93_09900"
     CDS             complement(<1..1358)
                     /locus_tag="VFV93_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX5165697.1"
                     /translation="MSQDPLARLRRTERREPRHRPTFPNDHYRSAFLDPGAETAGRTI
                     GEAAVTVYRAHAVVLAERRVVEADVAARMLRGREEMPDRADDLPIHRLAAVQNRVVAA
                     AGSAIQLGTAREEIALTAMRLVLRERLLAVADATLRLRAAITSVALQHLTTLLLATAN
                     GQVVQPTSLGHYLAGQLGPLVRTSARLEEAFGRLNLSPLGAVSGMATAVPVLRGRQAE
                     LLGFSGPIESTYDALAAADVLAEIAGIVSMMATELTRLIADLRFWARDDVGTMAPTDA
                     YVHGNESQPQRRDPLVLDHLRARLLDHAAVPQQLTLLLSDRQMLGGEASSYRAFDLVE
                     CQLAGVVETLDVLGEVVATADVNRALFAHRANRGFATSSELADLLSIDFEIPRDEAVQ
                     LAERVVVEATEAGGEATTLKTEVIDGIALKLRGVEVGIEPETLAKVLSPKRFIERRDH
                     PGG"
     gene            1504..2712
                     /locus_tag="VFV93_09905"
     CDS             1504..2712
                     /locus_tag="VFV93_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX5165698.1"
                     /translation="MTYDFPGKGKVAVLKTRPETVVDDFGQVMQLAGYQDSLDKALET
                     ILKINISWQTWYPACSTAPWQLEGVIRRLQADGYQNLAGAHNKTVVVDAYVGERNNKH
                     KYVVDKYGVRNVHLYEPEVEWVVYEPKGEMMVLNDIFPDGIKIPKWFIGKNMIQLPTV
                     KTHVFTTITGAMKNAFGGLLSERRHWTHAVIDETLVDLLTIQHEIHPGLFAVMDGTFA
                     GDGPGPRAMRVHEKDIILASADQVAIDAISAKIQGFNPLDLEFIRYAHEKGLGVGDPK
                     DIEIVGYDISAEDWGFIQEDTFASKGQKLIYHGPLKKYEKALLQSPIVPWSYFASNFY
                     HNVYWYPVVGRKRVKEALHSKWGQLFQSYGDGKVVLPGPEPVSASIAAGAGVAAVAGA
                     FLAGKTLLKK"
BASE COUNT          524 a          941 c          838 g          456 t
ORIGIN      
        1 ccgcccggat gatccctccg ctctatgaag cgcttcggcg acagaacctt cgcgagcgtc
       61 tccggctcga tgccgacctc gacaccgcgc agcttgagcg cgatcccgtc gatcacttcg
      121 gtcttcaggg tcgtcgcctc accgcccgct tccgtcgctt cgacgacgac ccgttcggcc
      181 aactgaaccg cttcgtcacg aggaatctcg aagtcgatcg agagcagatc ggccagttcg
      241 gagctggtcg cgaagccccg gtttgcccgg tgtgcgaaca gcgcccggtt gacatcggcc
      301 gtcgccacca cctcgccaag cacgtcgagc gtttcgacca cgccagcaag ctggcactcg
      361 accagatcga acgctcgata gctggatgcc tcgccgccga gcatctgccg gtccgagagc
      421 aacaaggtca gttgttgcgg aacagcggcg tgatccagca gccgcgccct cagatgatcc
      481 agcacaagcg gatcacgccg ctgcggctgg ctctcgttgc cgtgtacgta ggcgtcagtc
      541 ggcgccatcg tcccgacatc gtcccgcgcc cagaaacgca gatcggcgat caatcgtgtc
      601 agctcggtcg ccatcatcga gacgattccg gcgatctcag ccagcacatc tgccgcggcc
      661 agcgcatcgt aggtgctttc gatcggacca ctgaatccga gcagttcggc ctgccggcct
      721 cgcaggaccg gcacggccgt cgccatcccc gacaccgctc cgagcggcga caggttcagc
      781 cgcccgaacg cttcctccag acgggcgctc gtccgcacca gcggcccgag ctgccccgcc
      841 aggtagtgac ccaggctcgt cggctggacg acctggccgt tggccgtcgc gagcagcaac
      901 gtcgtcaggt gctgcagcgc taccgaggta atcgccgcac gcagccgcag agttgcgtca
      961 gccaccgcaa gaaggcgctc ccgcagcacc agacgcatgg ccgtcagggc gatctcctcg
     1021 cgagcggttc ccagttggat cgcgctcccg gccgccgcaa cgacccgatt ctgaacagcc
     1081 gccagccgat ggatcggcag gtcgtcggct cggtctggca tctcctcccg cccgcgcagc
     1141 atccgagcgg cgacgtccgc ctcaacgacg cgacgctcgg ccagcacgac ggcgtgtgcc
     1201 cggtaaaccg tcacggctgc ctcgccaatc gtccgcccgg ccgtttcagc acccggatcc
     1261 agaaacgccg atcgatagtg gtcgttggga aaggtcgggc gatgacgcgg ctcgcgtcgt
     1321 tcggttcgtc gcaatcgggc aaggggatcc tggctcacgg tggctctcaa tctggtccgg
     1381 cagtcgttgc gccataacgc tcggcaagcg ccgtgcgttg ttcaatcgta ccgaggttga
     1441 gtacgatcac gttcggcaac ctccggccag gtagacacca cagcagggag tagagcgaga
     1501 gccatgacgt acgactttcc gggtaagggt aaggtcgcgg tcctgaaaac gcgtcctgaa
     1561 acggttgtag acgacttcgg gcaggtaatg cagcttgccg gctatcagga ctctctcgac
     1621 aaggcacttg agacgatcct gaagatcaat atctcgtggc agacgtggta tcccgcctgc
     1681 tcgacggcgc cgtggcagct tgagggcgtc atccgccgcc tgcaggccga cggctaccag
     1741 aacctggccg gcgcccacaa caagaccgtc gtcgtcgatg cctacgttgg cgagcgcaac
     1801 aacaagcaca agtacgtcgt cgacaagtac ggcgtccgca acgtgcatct ctacgagccc
     1861 gaggtcgagt gggtcgtcta cgagccgaag ggcgagatga tggtcctcaa cgacatcttc
     1921 cccgacggca tcaagattcc caagtggttc atcggcaaga acatgatcca gctaccgacc
     1981 gtcaagacgc acgtcttcac aacgatcacc ggcgcgatga agaacgcttt cggcggcctg
     2041 ctttcggagc ggcgccactg gacccacgcc gtcatcgacg agacgttggt cgatctgctg
     2101 acgatccagc acgagatcca ccccggcctg ttcgccgtca tggacggcac cttcgccggc
     2161 gacggccccg gcccgcgcgc catgcgtgtc cacgagaagg acatcatcct cgccagcgcc
     2221 gaccaggtcg cgatcgacgc tatctcggcc aagatacaag gcttcaatcc gctggatctc
     2281 gagttcatcc gctacgcgca cgagaagggc ctcggcgtcg gtgacccgaa ggacatcgaa
     2341 atcgtcggct acgacatcag cgccgaggac tggggcttca ttcaggaaga caccttcgcc
     2401 tcgaaggggc agaagctgat ctaccacggc ccgctcaaga agtacgagaa ggcgctgctc
     2461 cagtcgccga tcgtgccgtg gagctacttc gcgtcgaact tctatcacaa cgtctactgg
     2521 tacccggtcg tcggccgcaa gcgcgtgaaa gaagcgctgc acagcaagtg gggccagctc
     2581 ttccagtcct acggcgacgg caaggtcgtc ctgcccggcc ccgagccggt ctcggccagc
     2641 atcgcggccg gagctggcgt cgcggcggtc gcaggcgcat tccttgcggg gaaaacgctg
     2701 ttgaagaagt aggcgacata gcaacctgat ccctccggta ggggcgaggc actgactcg
//
LOCUS       DASUGJ010000453         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1876687, whole genome shotgun sequence.
ACCESSION   DASUGJ010000453 DASUGJ010000000
VERSION     DASUGJ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876687"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFV93_09910"
     CDS             <1..778
                     /locus_tag="VFV93_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165699.1"
                     /translation="IDYTAGDEGTVGIMGSSVHDGVIAWDRWSTSDYRTEIRAAYLDQ
                     VLPSAAQPDPGTTGSHWTYFPETGHYLAWGFRDFWEVSGGLPVFGYPLTEEYIETNAD
                     TGADYSVQFTERQLFEWHPENAGTPYQVLLGRLGAEQAADLGLLDDEPFRYRGDDEGP
                     DAGCMYFRETGHYACDYFVFHWREHGLEMDDPGISYRESLALFGYPLSEPFMTTNSDG
                     DTVWTQYFERAVFELHPENVFPYRVLLRRLGAEVIDQRGW"
     gene            1274..2191
                     /locus_tag="VFV93_09915"
     CDS             1274..2191
                     /locus_tag="VFV93_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165700.1"
                     /translation="MLTADDGFAINPVWSPDGQHVAFERVKDPSQPEEMWQIAVAVMA
                     ADGSDVHDVGSGTTIVTTPSWSPDGTRIVYGATEHAADSSPAPAHITIVGVDGSDPHT
                     IIGPDVLGDDMVLSVDWSPDGSMIAFVRFAEPPSGSEPPADPSEPVITLWVAAADGTN
                     PRALSADAFIAAFFPAWSPDGSQLTFVGWEDPANTGIYVIARDGSDLRQIARGLHGDM
                     EKNEESIEGSILMAPSWSPDGNWVAFLAAEFHHDNHDTPTASAELQLVASDGSAQHTI
                     LDHLDVEVVMDPFDSMGSTAPVWQLGEHE"
     gene            2612..3226
                     /locus_tag="VFV93_09920"
     CDS             2612..3226
                     /locus_tag="VFV93_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165701.1"
                     /translation="MPFVDLTAAALQIDGASLTLTVDTGGPLPPALDAGDEMWWALEL
                     APSDPRSMTLYRVTVTFSGETWHTINYDFEHDARQILAQIDVGAGQLTVAIPLTELPK
                     LNGPFRWWVAALWGGFNLDRPDASGDRVPDGDAVMFPDGSQAAIPTWEDLPAAAAPLE
                     LGTVELPDDSATVEELISRLPEPNERLHLTSYADVTDEPADWQV"
     gene            3317..>3916
                     /locus_tag="VFV93_09925"
     CDS             3317..>3916
                     /locus_tag="VFV93_09925"
                     /inference="COORDINATES: protein motif:HMM:NF017874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vps62-related protein"
                     /protein_id="HEX5165702.1"
                     /translation="MLRRPLVRVLVPVFFLIVSLLLPSSALAGRPAGYDTTKEVTALI
                     ETYGPIVVYHPDEQYFPDTPEAVLGGPSRLVWGIVRNEDDFATFEQTILGSVETSADT
                     LLDDVAMAKQDPNSGDPDFRYWLDIDESLRAGHLQRAKTYVRVIPSGDLTLDLQFWFF
                     APYNGPIKGKAHVQIAIPGYPQPPDTYYYPDTVGRHSGDW"
BASE COUNT          738 a         1240 c         1216 g          722 t
ORIGIN      
        1 catcgactac accgccggcg acgagggaac agtcggcatc atgggcagtt ccgtccatga
       61 cggagtgatc gcctgggatc gctggtcaac ctccgactac cggaccgaga tccgcgccgc
      121 ctatctcgat caggtcttgc catccgcagc tcaacccgat ccgggaacga ccggctcgca
      181 ctggacttac ttcccggaaa ccggccatta tctggcatgg gggttccgcg acttctggga
      241 agtgagcggt ggattaccgg tcttcggcta tccactaaca gaggaataca tcgagacaaa
      301 cgccgacacc ggcgccgact actccgtcca gttcaccgag cgccagctct tcgagtggca
      361 ccccgagaac gccggtacac cctatcaggt gcttctcggc cggttgggcg ccgagcaggc
      421 tgctgacctc ggcctgctcg atgatgagcc gttccgctac cgtggcgacg acgaggggcc
      481 ggacgccggc tgcatgtact tccgcgagac cggccactat gcctgcgact acttcgtctt
      541 ccactggcgc gagcacggct tggagatgga tgatcccggc atctcctacc gcgagtcact
      601 ggcgctgttt ggctacccgc tcagcgaacc attcatgaca accaacagcg acggcgacac
      661 cgtttggacc cagtacttcg aacgggccgt cttcgagctc catccggaga acgtcttccc
      721 ctatcgggtg ctcctccgcc gtctgggagc cgaggtgatt gaccagcgag gctggtaacc
      781 gcaacgctga agacagaaat gggggttcgt cgtgaacggg ttgagtcgag gagcgatcgc
      841 cttgtcgctg ggcctgatga tggtcgtcgc ggcgtgtggt ggcagcggga ccgcggatcc
      901 gacagtgaca caagacacgg gaacgatgcc gacggctacg tcgaatacat caccggcacc
      961 gacacagacc ccgtcggccg ggacgagtac cggcaccaca gtctcgccga ccacgcctgc
     1021 actcacggct tcggggcaga tcctcctgcc tggggcgacg tctggatccg cagtcgacgc
     1081 tgggcttctc tcggtccatc atgatggcga gaatccgcat cgcataggcg cgggcggcac
     1141 gctgaacgcc tttcccagtt ggtcgctcga cggccggcac atcgcgttca cgcggttcct
     1201 gacgcctatg gacgggacca caataccccg agccgttatc gtcgcaacgg atgctgatgg
     1261 cacgcatgaa cggatgctta ctgctgacga cgggttcgcg atcaacccgg tctggtcgcc
     1321 cgatgggcag cacgttgcct tcgagcgggt taaggatcca tcccaaccag aggaaatgtg
     1381 gcagatcgcc gtcgcggtca tggccgccga cggatcagat gtgcacgatg tcggcagcgg
     1441 aaccaccatc gtcacgacac cgtcgtggtc gcccgatggg acacggatcg tctacggtgc
     1501 aaccgagcat gctgctgact cgtcgccggc gccggcgcac atcacgatcg ttggagtcga
     1561 cggcagcgat ccgcacacga tcatcggccc cgacgtgctc ggcgacgaca tggtcctctc
     1621 ggtcgactgg tcaccggatg ggtcgatgat cgcgtttgtc cgctttgccg agccgccgag
     1681 cgggagcgag ccgcctgctg atccatccga accggtgatc acactctggg tcgccgccgc
     1741 cgatggaacg aatccgcggg cgctatcggc tgacgcgttt atcgcagcct tctttccagc
     1801 ctggtcgccg gatggcagcc aactcacgtt cgtcggctgg gaggatccag ccaacaccgg
     1861 catctacgtg atcgcgcgag atggctcaga cctgcgacag atcgcgcgcg gattgcacgg
     1921 cgatatggag aaaaatgagg agtcgatcga gggatcgatc ctgatggcgc catcgtggtc
     1981 accggatggc aactgggtcg ccttcctggc tgcggagttc catcacgaca accacgatac
     2041 gccaactgcc agcgccgaac tgcagctggt tgcatccgac ggatcggcac agcacacgat
     2101 cctggatcat ctcgacgttg aggtggtcat ggacccattt gacagcatgg gaagcactgc
     2161 ccctgtctgg caactaggag aacacgagtg aggctaccgg cgacagcttt gatcacagtg
     2221 cgtcgtccca cacagggccg gggcacgact gcgcgccacg atccggccag gttgccatcg
     2281 ttcgttggac atcgattcac agtgcgctcg ccgaactcga ggtgatctga tggtccgagt
     2341 gagatacgta cttccgatcg tgctcatatt gatcatgacc gcctgtggag gcggtaacgg
     2401 tgatggtgcg gcgataccga ccgccgaacc ttcatccacg gtagcgcccg caactgccac
     2461 gcgagcgcca acggcatccc ccactccgaa acctgcgacc gaacccagcg gttccccgct
     2521 acccacaacg gttgtcccgc ctgggctgat gcagctggcg gacccatcgg gcgatgtcac
     2581 ggacgttcat ggggaaccgg ttgcgtacgc gttgccgttc gttgacctca cggcggctgc
     2641 gctgcagatc gacggcgcca gcctcaccct gacagtggac actggcggcc cgcttccgcc
     2701 ggccctcgat gccggcgacg agatgtggtg ggcgctggag ctggcgccat cggacccacg
     2761 cagcatgacc ctgtaccggg tgacggtcac gttctcgggt gagacatggc atacgatcaa
     2821 ctatgacttc gagcacgatg ccagacagat acttgctcag atcgacgtcg gcgcaggcca
     2881 actgacggtg gcgatcccgc tgacggagct cccgaagctg aatgggccat tccgctggtg
     2941 ggtggcagcc ctgtggggcg gcttcaatct cgaccgccct gacgcaagcg gcgaccgtgt
     3001 gccggacggt gacgcagtca tgttcccgga cgggtcccag gctgccatac caacctggga
     3061 ggatctgcct gctgccgcgg ctccgctgga gcttggcacg gttgaattgc ccgatgacag
     3121 cgccaccgtg gaggagttga tctcgcggtt gccggagccg aacgaaagac tgcacctgac
     3181 gtcttatgca gatgtaacag acgaacctgc cgattggcag gtttgacacg caatcgggcg
     3241 taagctgcga cttgccagcg tgtacgagtt ggagggtcac aaccgccaca cgttgggata
     3301 gagaggatgg tgtccgatgt tacgtcgacc gctggtccgc gtcctggtgc cggttttctt
     3361 cctgatcgtt agtctgctcc tgccctccag cgcgttggct ggaaggccgg ccggctatga
     3421 cacgaccaag gaggtaacgg cgctcatcga aacgtacggt ccgatcgttg tctatcatcc
     3481 cgacgaacag tattttccgg atactccgga agctgtcctg ggaggaccgt cacgcctcgt
     3541 ctggggtatt gtccgtaacg aggatgattt cgcgaccttc gagcagacga ttctcggctc
     3601 ggtcgagacc tcggccgaca cgctgctcga cgatgtcgcg atggcgaagc aggacccgaa
     3661 ctccggcgac ccggacttcc gctactggct cgacattgac gagagtctga gagccggaca
     3721 cctgcagcgc gccaagacct acgtgcgagt catcccgtct ggggatctta ccctcgatct
     3781 gcaattctgg ttcttcgcgc cgtacaacgg gccgatcaag gggaaggcgc acgtgcagat
     3841 cgctattcca ggctacccgc agccgccgga cacgtattac tatccggata cagtcggacg
     3901 tcattcgggt gactgg
//
LOCUS       DASUGJ010000454         5977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1739219, whole genome shotgun sequence.
ACCESSION   DASUGJ010000454 DASUGJ010000000
VERSION     DASUGJ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739219"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFV93_09930"
     CDS             complement(<1..692)
                     /locus_tag="VFV93_09930"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEX5165703.1"
                     /translation="MPRRRITLILLVLLMSMSVACMGDDDDDNNAASPTSNVTAADVL
                     ASARDTWDTTESAHFTLKVDGDAYLDDDETIKLVSAEGDIKRPGSVKAQASVDAQISV
                     VDISLVAVDGEIFITNLLSGNWEKAPDDFSYDPSILFSDTDGIGPIMTDLQDPKLDGT
                     EDVNGESAYKITGTVGADRVSAITAGTIAGDAIEVTLWTAVDDAKLLRVVLTEPKGVR
                     EAPATWNLDLTD"
     gene            877..1758
                     /locus_tag="VFV93_09935"
     CDS             877..1758
                     /locus_tag="VFV93_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX5165704.1"
                     /translation="MEDSRLVVGAATDPGPVREQNEDAVYHNVLVDTPGNELTLLAVA
                     DGMGGYQRGEVAAQMAIDTLIERFSSADTDDLVLMIKQAFRLANERIFAGGSAEGEHN
                     MMGTTLVVGALRGTDLVIGNVGDSRAYLVRANTLNQISTDHSLVAEQVAMGAMTQEEA
                     RESHHKNIITRALGHRQRVEVDIFELTLLPEDRLLISTDGLHDYLEDYEIVDLMTTLP
                     PEDSSREMVHRAIQAGSTDNVSALVAWAAPVSVLEAPPEAVLTGEPAGANRMITILVL
                     VGLVIFIAIIGYILLAT"
     gene            1770..2639
                     /locus_tag="VFV93_09940"
     CDS             1770..2639
                     /locus_tag="VFV93_09940"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5165705.1"
                     /translation="MTGRIIDAHTHIFPPDVVARREAYHSRDAWFSLCYENPKLLLAS
                     PEDLIASMDEAGIEMSIACGFPWADPGICREHNDWMAEACRAYPDRLAFLAIVAPHQP
                     GAVTEAERAFGLGAAGIGELNADAQGFDLTAPETVRDLMALCHDLQKPVMLHSTEPIG
                     HIYPGKGSATPERLVVWLTAYPAQPVVLAHWGGGLPFYELMPEVRAVTRFVAYDSAAT
                     TYLYNHAVFEHVIRLVGEERVLFGSDYPILRQDRLARRVARQIEDVDTLADVYAGNAS
                     RIYGLNRQGEGQA"
     gene            2636..3217
                     /gene="ruvA"
                     /locus_tag="VFV93_09945"
     CDS             2636..3217
                     /gene="ruvA"
                     /locus_tag="VFV93_09945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5165706.1"
                     /translation="MIAGLRGQIGRIEPAAVVVDLHGFMLRVQTSNRVLASLGAPGDP
                     IELLTHLAVREDALTLYGFPTQAELDLFELLLTVSGVGPKVALALLSFGEPSSLYEAI
                     ANEDTALLSKVPGIGKKTAEQIVFHLRRKLPDFVPAGTSPADDGDREAVTALEALGYT
                     LPEARSALAAVEQRGGMTVEERVYNALQRLAKS"
     gene            complement(3343..4686)
                     /gene="hisD"
                     /locus_tag="VFV93_09950"
     CDS             complement(3343..4686)
                     /gene="hisD"
                     /locus_tag="VFV93_09950"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX5165707.1"
                     /translation="MVVRLFTDADAGRAFVLRNRALGEATLSPRMRDGIRQVFGEDLS
                     AEQVVSRIIAGVRANGDEALRHYTEAIDGVSLDAFEVTRDEIEAALATVPADVAEALR
                     FAATRIRRFHEQQHIDSWMDFQPEGALGQIIRPLERVGLYAPGGTAPYPSSLLMTAIP
                     ASVAGVQEIVVCAPPERDGRIAPVTAVAAHVAGVQRVFKLGGAQAIAAMALGTASVPK
                     VDKILGPGNIFVVLAKRQVYGEVAIDALPGPTETLLIADESADTALCAADMLAQAEHD
                     PMASAILLTTSAVLAERVRDELDRQQASLSRASIAVESLESNGAIVVVSSLEDAFEIA
                     NAYAPEHLCLLLADPWSHVASVKHAGGIFVGEHSPEVIGDYTAGPSHVMPTGRTARFS
                     SPVNVSDFVKVISVIGLNERGLREVGPAAVRIAHAEGLTAHAAAVERRLERLRPE"
     gene            complement(4680..5723)
                     /gene="hisG"
                     /locus_tag="VFV93_09955"
     CDS             complement(4680..5723)
                     /gene="hisG"
                     /locus_tag="VFV93_09955"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX5165708.1"
                     /translation="MRDGSHLRIALTSKGSYEESTRRFLDGAGLSVWRPNPRQYVGRM
                     SAIPAAEVLFQRTEDIVHKVADGSADIGITGFDLVSEFTSDDPNTHVVIQDLGFRRCQ
                     LVIAVPESWLDITTIDDLAELSIQFKRDGRQLRIATKFQNLVSDHLYRNGVNYFTLTA
                     AHGALEAAPALGYADVIADLTETGVTLRDNHLRVIEGGVVLRAQACMIANLRTIGATT
                     ERLEQARMFIELCEAKLRSRSYRVITANIAASSPESVAEQIISNIDLAGETGPTVAPV
                     FPKQAGSERWFSVSVIVPTERMMAAVDHLRLAGSRGITVSTPEYMFDTESGAFARLKE
                     AVAARCKGAGATW"
     gene            complement(5720..>5977)
                     /locus_tag="VFV93_09960"
     CDS             complement(5720..>5977)
                     /locus_tag="VFV93_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165709.1"
                     /translation="PAVLVLQGDDPAGALALADGFRAAGWQANVDLRERNQSATRRVA
                     QRQGYAALARRTEERVELHWLADGRTVTFDGIPTPDEAGGR"
BASE COUNT         1022 a         1979 c         1945 g         1031 t
ORIGIN      
        1 tggtctgtga gatcgagatt ccaggttgcg ggcgcctcac ggacgccctt cggctcggtc
       61 agcacgaccc gcaacaactt ggcatcgtca acggctgtcc agagcgtaac ctcgatcgca
      121 tcgcccgcga tcgtgccggc cgtaatagcc gagacccggt cggctccgac tgtcccggtg
      181 atcttgtagg ccgattcacc gttaacatct tcggtcccgt cgagctttgg gtcctgcaag
      241 tcggtcatga tcgggccgat cccgtccgtg tcgctgaaga ggatggatgg atcgtagctg
      301 aaatcgtcgg gagccttctc ccagttgccg ctcagcaggt tggtgatgaa gatctcgccg
      361 tcgacggcga ccagcgagat atcgacgacc gagatttgcg cgtcgacact ggcctgagcc
      421 ttgactgacc cgggccgctt gatatcgcct tcggcagaga cgagcttgat cgtctcatcg
      481 tcgtccagat aggcatcgcc gtcgaccttc agcgtaaaat gggcactctc ggtcgtatcc
      541 caggtgtccc gcgccgacgc aagcacgtcg gccgcggtga cgttgctggt cgggctggcc
      601 gcgttgttgt cgtcgtcatc gtcacccatg caggcaactg acatgctcat cagcaggacc
      661 agtagaatca acgtgatacg tcgtcgtggc atcgataccc ctcgctacag gtgaactgtt
      721 tcacgcgtcg tggccgagga tgtgcaaacg agggcgcaaa cgcgaaacag tgtcgtatta
      781 taggcgggtg tacgcactat ccgttccaag caggggtatc agacttcgag tggcgccgca
      841 gcgcggcctg gcgtgtaact gaggagagtc gccgacgtgg aggacagccg gctggtcgta
      901 ggtgcggcaa ctgaccccgg tcccgtccgg gagcaaaatg aagatgccgt ctaccacaac
      961 gtgttggtgg atactcccgg gaacgaactg acgctgctgg ccgtcgccga cggtatgggc
     1021 ggttaccagc gcggagaggt cgccgcccag atggcgatcg acacgctgat cgagcgcttc
     1081 agcagcgccg acaccgacga cctcgtcctg atgatcaaac aggccttccg gctggccaac
     1141 gagcgcatct tcgccggtgg ctcggccgag ggcgaacaca acatgatggg cacgacgctg
     1201 gtggtcggcg cgctgcgcgg gaccgacctc gtcatcggca acgtcggcga cagtcgcgcc
     1261 tacctcgtgc gcgccaacac gctcaaccag atcagcaccg atcactcgct ggttgccgag
     1321 caggtcgcga tgggcgcgat gacgcaggaa gaagcgcgcg agagccacca caagaacatc
     1381 atcacgcgcg ccctcggcca tcgccagcgc gtcgaggtcg atatcttcga actgacgctg
     1441 ctgcccgagg accggctgct gatctcgacc gacggcctgc acgactatct cgaagactac
     1501 gagatcgtcg atctgatgac gacgctgccg ccggaggact cctcgcgtga gatggtccac
     1561 cgagctattc aggccggctc gaccgacaac gtctcggcgc tggtcgcctg ggcagcaccg
     1621 gtcagcgtcc tcgaagcgcc gccggaggcg gtgctgaccg gcgaaccggc cggcgcaaac
     1681 cggatgatta cgattctcgt cctggtgggt ctggtgatct tcatcgcgat catcggctac
     1741 atcctgctgg cgacctagaa cggccactga tgacgggccg gatcatcgac gcgcataccc
     1801 acatcttccc gcccgacgtc gtcgcgcggc gcgaggcgta tcactcgcgg gacgcctggt
     1861 tcagcctctg ctacgagaac ccgaagctgc tgctggcgtc gcctgaagac ctgatcgcca
     1921 gcatggacga ggccggcatc gagatgtcga tcgcctgcgg gttcccgtgg gccgatccgg
     1981 gcatctgccg cgagcacaac gactggatgg ccgaggcgtg ccgggcctac ccggaccggc
     2041 tggcgttcct ggcgatcgtc gccccgcacc agcctggcgc ggtgaccgag gccgaacgcg
     2101 ccttcggtct cggcgcggcc ggcatcggtg agctcaacgc cgacgcgcag ggcttcgacc
     2161 tgaccgcgcc ggagacggtc cgcgacctga tggcgctctg tcacgacctg caaaagccgg
     2221 tcatgctcca ttcgaccgaa ccaatcggcc acatctaccc cggcaaggga tcggcgactc
     2281 cggagcgatt ggtcgtctgg ctgactgcct atccggccca gccggtcgtc ctcgcccact
     2341 ggggcggcgg gctgccgttt tacgagttga tgccggaagt cagagccgtt acccgcttcg
     2401 tcgcctacga ctcggccgcg acgacctacc tctacaacca cgccgtcttc gagcatgtta
     2461 tccggctggt tggcgaggaa cgagtcctct tcggaagcga ctatcccatc ctgcggcagg
     2521 atcggctcgc gcgccgcgtg gcacgacaaa tcgaagacgt ggacacgctg gcggatgtct
     2581 acgccggcaa cgccagccgg atctacggcc tcaacaggca gggagaggga caagcgtgat
     2641 tgccgggcta cgcggccaga tcgggcggat cgagccggcc gccgtcgtcg ttgacctgca
     2701 cgggttcatg ctgcgcgtcc agacatccaa tcgggtgctg gcaagtctcg gcgcgcccgg
     2761 cgacccaatc gagctgctga cgcacctcgc cgtccgcgag gatgcgctga cgctctacgg
     2821 ctttccgacc caggcggagc tggacctgtt cgagttgctg ctgaccgtca gcggcgtcgg
     2881 gccgaaggtc gcgctggcgc tgctgtcgtt cggcgaaccc tcgtcgctct acgaggcaat
     2941 cgccaacgag gacaccgcgc tgctctcgaa ggtgcccggc atcggcaaga agacggccga
     3001 gcagatcgtc ttccacctgc gccgcaagct gcctgatttc gtcccagccg gaacgtcccc
     3061 cgccgacgac ggcgaccgcg aggccgtcac cgccctcgaa gcactcggct acaccctccc
     3121 cgaagcccgc tccgccctag ccgccgtcga acaacgcggc ggcatgaccg tcgaagaacg
     3181 ggtctataac gcactgcaac ggttggcgaa atcgtaacta aaggccgtct gcacgtaggg
     3241 gcgagtcagt gcctcgccct tcgtgtcccc accaggccgt tcgggcgggg agctgggcga
     3301 gtcagtgcct cgcccctgcc ggaggggatc tacgatgcag ctctactcgg gacgcaagcg
     3361 ttcgagacgc cgttcgacag cggcggcgtg ggcggtcagc ccttcggcgt gggcaatgcg
     3421 gacggcggcg ggaccaacct cgcgcaggcc gcgttcgttg aggccgatga cggagataac
     3481 tttgacgaag tcgctgacgt tcacaggtga cgagaagcgt gccgttcgcc cggtcggcat
     3541 gacgtggctc gggccggccg tatagtcgcc gatgacttcc ggcgagtgct cgccgacgaa
     3601 gatgccgccg gcgtgcttga cgctggcgac gtgcgaccac gggtcggcca gcagcaggca
     3661 gaggtgctcc ggcgcgtagg cattggcgat ctcgaacgcg tcttcgaggc ttgacacgac
     3721 gacgatcgcg ccgttgcttt ccagcgactc gacggcgatg cttgcgcggc tgagagacgc
     3781 ttgctgtcga tccagttcgt cacgtacccg ttccgcgagt acagcagagg tcgtcagcag
     3841 gatcgcgctc gccatcgggt cgtgttcggc ctgcgccagc atatcggcgg cgcagagggc
     3901 agtgtcggca gactcgtcgg cgatcagcag cgtttcggtt ggtcctggca gggcgtcgat
     3961 ggcgacttca ccataaacct gccgcttggc cagcacgacg aagatgttgc cggggccgag
     4021 gatcttatcg actttcggaa ccgatgcggt cccaagcgcc attgcagcga tcgcctgggc
     4081 gccgccgagc ttaaagacgc gctgcactcc ggcgacgtgg gccgcgacgg ccgtcaccgg
     4141 cgcgatccgt ccgtcccgtt cgggcggggc gcagacgacg atttcttgaa caccggcaac
     4201 cgaagccggg atggccgtca tcagcagcga cgacgggtac ggcgccgtcc cgcccggcgc
     4261 gtagaggccg acccgctcca gcgggcggat gatctgtccc agcgcgcctt ccggctgaaa
     4321 gtccatccac gagtcgatgt gttgttgctc gtgaaagcgg cggatgcgag tagccgcgaa
     4381 acggagtgct tctgccacgt cggccggcac agtcgccagt gctgcttcga tctcgtcacg
     4441 cgtgacttcg aacgcgtcga ggctgacgcc gtcgatcgcc tccgtgtagt ggcgcaacgc
     4501 ctcgtcgccg ttcgcgcgca cgccggcgat gatccggctg acgacctgct cggcgctcag
     4561 atcttcgcca aacacctgcc ggatgccgtc ccgcatgcgt ggcgagagcg tcgcctcgcc
     4621 cagcgcccgg ttgcgcagca cgaaggcccg gccggcgtcc gcgtcggtaa acagccgcac
     4681 taccacgttg cgccagcccc tttgcagcga gccgcgaccg cctccttcag tcgggcgaac
     4741 gcgcctgatt cggtgtcgaa catgtactcc ggcgtactga cggtgatccc ccgcgatccg
     4801 gccagccgca ggtgatcgac ggccgccatc atccgctcgg tcgggacgat gacgctgacg
     4861 gagaaccagc gttcgctgcc ggcctgcttc ggaaagaccg gcgcgacggt tggcccggtc
     4921 tcgccggcca ggtcaatgtt ggagatgatc tgctcggcga ccgactccgg cgagctggcc
     4981 gcgatattgg cggtgatgac ccgatagcta cgcgaccgca gtttcgcctc gcacagctca
     5041 atgaacattc gcgcctgctc cagacgttcc gtcgtcgcgc caatcgtccg caggttggcg
     5101 atcatgcagg cctgggcgcg caagacgacg ccgccctcga tcacacgcag gtggttgtca
     5161 cgcaacgtca cgccggtctc ggtcaggtca gcgatgacat cggcgtagcc gagcgcgggc
     5221 gccgcttcga gcgcgccgtg ggcagctgtc aatgtgaagt agttgacgcc gttgcggtag
     5281 agatggtcgc tgaccaggtt ctggaacttg gtcgcgatcc gcaactggcg gccatcacgc
     5341 ttgaactgga tcgagagctc agccagatcg tcgatcgtcg tgatgtcgag ccacgactct
     5401 ggaacggcga tgacgagctg acagcgccga aatcccaggt cctggatgac gacatgcgtg
     5461 tttggatcgt ccgacgtgaa ctcggagacg aggtcgaagc cggtgattcc gatgtcggcg
     5521 ctaccgtcgg cgaccttgtg aacgatgtct tccgtgcgct gaaacagcac ctcggcggcc
     5581 gggatcgctg acatccgacc aacatactgg cgtgggtttg gccgccagac gctcagcccg
     5641 gcgccgtcaa gaaagcgacg ggtcgattcc tcgtaggagc ccttgctggt cagcgcgatc
     5701 cgcaggtgcg agccgtccct catcgtccac ctgcctcgtc tggcgttggg ataccatcga
     5761 atgtcaccgt ccggccatcg gccagccaat gcagctcaac gcgctcttcc gtgcgccggg
     5821 cgagtgccgc gtagccctgc cgttgcgcga cacgcctggt tgctgactgg ttgcgctcgc
     5881 gcaggtccac attcgcctgc cagccggctg ccctgaatcc gtccgcgagc gccagcgcgc
     5941 cggccgggtc gtcgccctgc aacaccagca cggccgg
//
LOCUS       DASUGJ010000455         8177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2016797, whole genome shotgun sequence.
ACCESSION   DASUGJ010000455 DASUGJ010000000
VERSION     DASUGJ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2016797"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2001)
                     /locus_tag="VFV93_09965"
     CDS             complement(<1..2001)
                     /locus_tag="VFV93_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5165710.1"
                     /translation="MSIPSPSPVPSGALDDRRLRAALRLFLPPDRADSAASDIMAAAD
                     TTSGLRQLAGDLARERWLKRVRASLSMQSTGATLPAGASERALLMLVVHLGLDLEGEW
                     LEDAFGLDGGEAGRELLRARAALSGQSLPACRECDWMIGRYRDRSLPADDRLRLLSHV
                     TTCERCRSALDRAQQIDADLCVAINQREAALPDRPVAMPASRPKRAAGNMTVTVGAVV
                     ALLAIVVGFSVIGDLFSNPRPAVPLFVNESVSTAPLDGWLLQVSTVGYLEGVNLSNGR
                     KRGLDSGDGGNFSPLYRVSKTHIAAWRPSDGQRDDVLTIGPIGDQFRFRLTWNRRFVY
                     WYPSGWLDDDTLLIVKSPEHIDGETEQAYIDRLARDSRLIAFDAQTGAEQVLMTGNVA
                     AAFPSPDGTMLAIIQPVDRRWPGNSLELRPFDGRHVGEPIAQIDHRVAADGVWLADSS
                     RFVASVITDETVSRLEAISDASLAQRGVSGVALVAIDRNGGQQTLVSATIPETIVPMA
                     ASPDGRSVVYEVRKERPRSAFPGIPDYAWRCYQLMLSGGEPALLASGESPERTFTPAW
                     SPDGTTLVLPVARGFPLATDDRLTPPIIPNATALLVFRPGTDPEVIYSSGRDLYGWLG
                     PEALLPRAGTGSDGSRTLASVELTEVDLDRLSIDARSTASGGD"
     gene            complement(2099..4675)
                     /locus_tag="VFV93_09970"
     CDS             complement(2099..4675)
                     /locus_tag="VFV93_09970"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX5165711.1"
                     /translation="MYGRRVTIGCLLLLLTTLVPAVPPRTASAAQPDICGPALDRWVV
                     APEDRAVRPVAIERADANGGRVENPDGLLAAGGAEATLERVSGSDPTIILDFGKVVSG
                     QVRITTGASNQPPLRIAASESLQFLTDDGDVDWGDQRSYGWQPARAGDEYLSGQLTFR
                     YLMLYLAGNGRVDIDDVVLEFTPFLGTSDTFAGCFESSDDLLNRIWYAGAYTLELNTV
                     SQSDGETLILDGAKRDRAVWIGDLAVAARMEYATHNQPDGIRDSLAAMADRQRPDGTI
                     PPSSFLDGSLIMYDYYAWWVTTFAEYYLYTGDAAFARTYYAQMQRQLDWFTGRIGPNG
                     LIQKDAGIEWAFTLGRNGEVTYINAVYYNALLQAAQLADALGHGDHAASWRARAASIK
                     QAINARLWDAGRGVYVDSDLDRNHVPQDGNALAVLYGIASPEQARTVLDYLQRTMWTP
                     FGATNVDVPYGTNLIHDKRIWPFMGYFEVEARFAAGDDARAYDLLRREWGQMLNSDPA
                     STMWEWMRADGSIENGFTSLAHAWSGGVTASLTEHTLGIQRTGPQFATFDAIPSPGDV
                     GWAKGRVPTSRGNIDVSWTASDGVFAETLTVPGGSRARAGVPLLGRQPVVQIDGRVVW
                     DGRGSVGMDATNDGRYVYVELGPGTHRLESISDAYYFPETGYILHGVFKAFWERSGGV
                     PVFGYPVSRQSAGVEIPTQYFERQRFEYHPENMGTDYEVLLGLLGSTDAERRGLLGTP
                     AFQPAQPDPDPNCRYVPETGHNICDGFRGYWEQHGLELGDNGVSDRESLALFGYPISE
                     EFIDPESGLTTQYFERAKFEFQPENPGPFTVLLGRLGASIVDRDLLKLTRSQRPGLGL
                     IP"
     gene            complement(4984..5226)
                     /locus_tag="VFV93_09975"
     CDS             complement(4984..5226)
                     /locus_tag="VFV93_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5182..5184),aa:Sec)
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HEX5165712.1"
                     /translation="MADKRPLSIEYCTSUGYLKKAASLAEQLLDDHGNKLTNVTLIPS
                     SGGVFEVTFGDHLIYSKKETGQFPEPEKIAETVAAG"
     gene            5394..5885
                     /locus_tag="VFV93_09980"
     CDS             5394..5885
                     /locus_tag="VFV93_09980"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX5165713.1"
                     /translation="MASQNSFDVVSKFDAQELRNAVDQTMREVGTRYDLKDSKSQIEQ
                     EDTQLIINTDSEMSLRAIHDILESKLVRRNLSLKILDYQEEEPASGGRVRQTVKLRQG
                     LNDDLAREISKRIRTEFKKVTAQIQGDLIRVSAKNRDDLQSVIQALKESDYPVPLQFV
                     NYR"
     gene            complement(5882..6961)
                     /locus_tag="VFV93_09985"
     CDS             complement(5882..6961)
                     /locus_tag="VFV93_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165714.1"
                     /translation="MASRPDSARAAIPTRRPTMRQRLGRFAARVASHITRVIPLRVGY
                     MVCDRVGDLLYWRSRRYRINVIENLRHVYGPSIGELMLRRYARRVFRTSARNFWDLTR
                     VPHLTVRDFERMVRLPANDWSLFDRIREEGKGGIIMTAHLGAFDFVGQTLFMRGYNPY
                     TLTSPTVGEFVYAAVNYLRHSQNAPLEDISPAAIRRMLRVLLKGGFAGVVADRDFTDS
                     GGPATFFGVETTLPLGPVRLARVSGAPILPVFAIREDDGGRRQRYAFHLLDPVYVERT
                     ADEDADVSAGLAKMVAVLEQGISMAPDQWVMFQRVWDDEQPRRRALLDRLAAASAARQ
                     AAADEQASATNADTAPQGAAAESDD"
     gene            complement(6945..7643)
                     /locus_tag="VFV93_09990"
     CDS             complement(6945..7643)
                     /locus_tag="VFV93_09990"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5165715.1"
                     /translation="MANSITVGRVVLLFVAIAMVYSGNYWTIVVAGVLIGAIFAGDGL
                     DGGVARRRNSSSKLGAVLDIAGDRVVENVLWVVFAEMQIVGVWAPLIVLTRSFVVDVL
                     RSVALTEGKTPFGETTMARSRLTWFLTASRFSRTAYGWSKAIGFVFLTWLVAWRLPDA
                     DGRILDTLYDYQIVRGIGWTLVYASVALCVIRGLPVIWDARYYLREAEEPYPAGREPG
                     TLLSTERTERGEPA"
     gene            complement(7854..>8177)
                     /locus_tag="VFV93_09995"
     CDS             complement(7854..>8177)
                     /locus_tag="VFV93_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165716.1"
                     /translation="LLGDQIASALVAAGVVLPVLMVAYLYSQYEGLPDQIPIRWDATG
                     DVIGQTAPSGLWRFPRIAVVVLIVNTALATLLIDLDRYLARLLLSGIPLVQLILFIAL
                     VRAAS"
BASE COUNT         1515 a         2685 c         2667 g         1310 t
ORIGIN      
        1 gtcgccgccg gaggccgtcg agcgggcatc aatcgacagt cggtccagat ccacctccgt
       61 caactcgacg gacgccagtg tgcggctgcc atcactgcca gtccctgccc gcggcagaag
      121 cgcctcaggg ccgagccagc catagaggtc ccgaccactg ctgtagatca cctccggatc
      181 ggtgccgggg cggaacacga gcaacgccgt cgcgttcggg atgatcggcg gggtgaggcg
      241 atcgtcagtc gcgagcggga atccccgcgc taccggcagc accagcgtcg tgccatccgg
      301 cgaccatgcc ggcgtgaagg tccgttcggg actctccccg ctcgccagca acgccggctc
      361 accgcccgac agcatcagct ggtagcagcg ccaggcgtag tccgggatgc cggggaacgc
      421 ggatcgtggc cgttccttgc gcacctcgta gacgaccgag cgtccatcgg gagacgccgc
      481 catcggcacg atcgtctcgg ggatcgtcgc cgatacgagc gtctgttgcc cgccgttgcg
      541 gtcgatggcg accaacgcga cgccactgac tccccgttgg gcgagactcg cgtcgctgat
      601 cgcctccagc cggctgactg tctcgtcggt gatcaccgat gcgacgaagc gggagctgtc
      661 ggccagccag acgccatccg ccgcaactcg atggtcgatc tgcgcgatcg gctcgccgac
      721 gtgacgcccg tcgaacggtc gtagctccag cgagttgccg ggccagcgcc gatcgaccgg
      781 ctggatgatc gccagcatcg tcccatccgg cgacgggaac gctgccgcga cgttgccggt
      841 catcagcacc tgttcggcgc cggtctgcgc gtcgaacgcg atcagccgac tgtcgcgtgc
      901 caggcggtcg atgtacgcct gctcagtctc accgtcgatg tgctcgggcg atttcacgat
      961 caggagcgtg tcgtcgtcca gccaaccgga tgggtaccag tagacgaagc ggcggttcca
     1021 ggtcaaccgg aaccggaact gatcgccgat cggtccgatc gtcaacacgt cgtcgcgctg
     1081 gccgtcggac ggccgccagg cagcgatgtg cgtcttactg acgcggtaga gcggcgagaa
     1141 gttgccgcca tcaccactgt cgagcccgcg cttcctgccg ttcgacaggt tgacgccctc
     1201 caggtagcca acggttgaca cctgcagcag ccagccatcg agtggcgcag tcgagacaga
     1261 ctcgttgaca aacagcggga ccgccggtcg cggatttgag aagaggtcgc cgatgacgga
     1321 gaatccgacg acgatcgcca gtagcgccac gacagcgccc acggtgacag tcatgtttcc
     1381 tgcagcccgt ttcgggcgac ttgcgggcat tgcgacagga cgatcaggta gcgccgcctc
     1441 gcgctgattg atcgctacac acaagtccgc gtcgatctgc tgtgcccgat cgagcgcaga
     1501 ccgacatcgc tcgcaggttg tgacatggga gagcagccgc aggcgatcgt cggccggcag
     1561 gctccggtcg cggtagcgac cgatcatcca gtcgcattcg cggcaggcgg ggagcgactg
     1621 gccggaaagc gcggcacgtg cccgcagcaa ctcccggcct gcctccccgc catcgagacc
     1681 gaacgcatcc tccagccact caccctcaag gtcgagaccc aggtggacaa ccagcatcag
     1741 caacgcgcgc tctgaagcac ccgccgggag cgtggcccct gttgactgca tcgacagcga
     1801 ggccctgaca cgcttcagcc agcgttcgcg cgccagatca ccggccagct ggcggaggcc
     1861 gctggtggtg tcggcagcgg ccatgatgtc ggaggcggcg ctgtcagcgc ggtcaggcgg
     1921 gaggaacagg cgcagggcgg cccgaaggcg gcggtcgtcc agcgcgccgc tcggaaccgg
     1981 cgatggcgag ggtatcgaca tgctcgcccg cttcctgctg cgaggtcgaa atcgagatcg
     2041 ggatgaacgg ttcgccgcga tcatgcgacg gacgagacac aattgtcaat aaatgtacct
     2101 acggtatgag accgagtccc ggacgctgcg agcgtgtcag cttgagcagg tctcgatcga
     2161 cgatcgaggc gccaagccgg ccgagcagga cggtgaacgg acccggattc tcgggctgga
     2221 actcgaactt tgcccgctcg aagtactgcg ttgtcagccc ggattcggga tcgataaact
     2281 cttcggagat gggatagccg aacagagcca gcgactcgcg gtctgagacg ccattatcgc
     2341 cgagttccag cccgtgctgc tcccagtagc cgcggaagcc atcgcagatg ttgtgcccgg
     2401 tctcgggcac atagcggcag ttcgggtcgg ggtcgggctg ggccggctgg aatgcgggcg
     2461 tcccgagcag gccgcgtcgc tcggcgtcgg tgctgcccag cagccccagc agcacctcgt
     2521 agtcggttcc catgttctcc ggatgatact caaagcgctg gcgctcgaag tactgagtcg
     2581 ggatctcgac gccagctgac tgtcgcgaga ccggatagcc gaagaccggc acaccgccgc
     2641 tacgctccca gaaggccttg aagacaccgt gcaggatata cccggtctcg gggaagtagt
     2701 aggcgtccga gatcgactcc agccggtgcg tgccggggcc gagctcaacg tagacgtagc
     2761 gaccatcgtt ggtcgcgtcc atcccgaccg agccacggcc gtcccagacg acgcgaccgt
     2821 caatctggac gaccggctgc ctgccgagta acggcacacc agcccgcgcg cgactcccgc
     2881 ccgggacggt cagcgtctcg gcgaagacac catccgacgc cgtccacgac acgtcgatgt
     2941 tgccgcgcga ggttgggacg cgccctttcg cccagcccac gtcgccgggc gacggaatgg
     3001 cgtcgaaggt cgcgaactgc gggccggtgc gctggatgcc gagcgtgtgc tcggtcagcg
     3061 aggccgtcac gccgcccgac caggcatggg ccaggctggt gaagccgttt tcgatgctgc
     3121 cgtcggcccg catccactcc cacatcgttg aggccgggtc gctgttcagc atctggcccc
     3181 actcgcggcg cagcaggtcg taggcgcggg cgtcatcgcc ggccgcgaag cgtgcctcca
     3241 cctcgaagta gcccatgaac ggccagatcc gcttgtcgtg gatcaggttc gtgccgtacg
     3301 gaacatcgac gttggtcgcg ccaaacggcg tccacatcgt ccgctggagg tagtcgagga
     3361 ccgtccgcgc ctgctcaggg gacgcgatgc cgtaaaggac cgccagcgcg ttgccatcct
     3421 gcgggacgtg gttgcggtcg agatccgaat cgacgtagac accacggccg gcgtcccaga
     3481 gccgcgcgtt gatggcctgc ttgatactgg ccgcccgcgc acgccagctg gcggcgtggt
     3541 cgccgtgacc gagtgcgtct gccagctgcg cagcctgtag cagcgcgttg tagtagacgg
     3601 cgttgatgta ggtcacttcc ccgttgcgcc cgagcgtaaa ggcccactcg atgccggcgt
     3661 ccttctggat caacccattc gggccgattc ggcccgtgaa ccagtcgagc tgccgttgca
     3721 tctgggcgta gtaggtccgt gcgaaggcgg cgtcacctgt gtagaggtag tactcggcga
     3781 acgtcgtcac ccaccaggcg tagtagtcgt acatgatcag actgccgtcg aggaacgaag
     3841 acggcggaat cgtcccgtcc ggccgctgcc gatcggccat cgccgccagc gagtcgcgga
     3901 tgccgtccgg ctggttgtgg gtcgcgtatt ccatccgtgc cgccacggcc aggtcaccga
     3961 tccagacggc gcggtcgcgc ttggctccat cgaggatgag cgtctctccg tctgattggc
     4021 tgacagtatt cagctccagc gtgtaggcgc ccgcgtacca gatccggttc agcaggtcgt
     4081 cgctggattc gaagcagccg gcgaacgtat cggacgtgcc gagaaacggc gtgaactcca
     4141 ggacgacgtc atcgatatcg acccgtccgt tgccggccag atagagcatc aggtagcgga
     4201 aggtgagttg acccgagaga tattcgtcgc cggcccgcgc cggttgccag ccgtacgagc
     4261 gctggtcgcc ccagtcgaca tcaccatcgt cagtaaggaa ctgcaacgac tcgctggccg
     4321 cgatccgcag tggcggctgg ttcgacgcgc cggtcgtgat ccgaacctga ccgctgacga
     4381 ccttgccgaa gtcgaggatg atggtcgggt cgctgccgct cactcgctcc agggtcgcct
     4441 ccgcgccgcc ggccgccagc agcccgtcgg gattctcgac cctgccgccg ttcgcgtcgg
     4501 cccgctcgat cgcgaccggc ctgactgcgc ggtcctcggg cgcgacgacc cagcgatcga
     4561 gtgccgggcc gcagatatcc ggctgtgctg cgctggcagt ccgcggcggg acagccggga
     4621 cgagcgtcgt gaggagcagc agaagacagc cgatagttac gcggcgacca tacacgtggc
     4681 gacaaaccct tatacgatgc gaccgtttcc ggttgaccgg cacacctgag tccgggacgc
     4741 ctgtcagtca gacactgcgc cacgcgacgc cgctccaccg agcggccgga cccggatacc
     4801 gtatggcaca gcctaccaga caacacgggc tatcgcgcat accgatttgg tcctgcattt
     4861 ttgagtcacg gcgatgcaca caggaatgcc ggccccgcct gacgcgagac cggcatccgg
     4921 atgcaggcga gatcggctgc aagcaaccaa catcatggcg catcagcacg cttcaaggcg
     4981 ccttcagccg gcggcgaccg tctcggcgat cttctccggt tccgggaact ggcccgtctc
     5041 cttcttggag tagatcagat ggtcgccgaa agtgacctca aacacacccc cactgctggg
     5101 gatgagggtc acgttggtga gcttgttgcc gtggtcatcc aggagttgtt ccgccagact
     5161 ggcggctttc ttgaggtatc ctcaggaggt gcagtattcg atcgagagcg ggcgtttgtc
     5221 tgccattcag tcctctcctc tcaacacgcg cggccagcat gccgcatcgg ttccttttac
     5281 agcaaccttg ctgccatgtc cattcccaac gtgcacgtac gcgcctccat tgttcgatcg
     5341 atcccgcttg ctatccttat cggcgaggtt tcacgggagg gagtcagatc acaatggcat
     5401 cacagaactc attcgacgtt gtctccaagt tcgacgcgca ggagctgcgt aacgcggtcg
     5461 atcagacgat gcgcgaggtc ggcacgcgct acgacttgaa agactcgaag tcgcagatcg
     5521 agcaggaaga cacccagctc atcatcaaca ccgacagcga gatgtcgctg cgggcgattc
     5581 acgacatcct cgagagcaag ctcgtgcggc gcaatctgtc gctgaagatc ctcgactacc
     5641 aggaggaaga gccagccagc ggcggccgcg ttcgccagac ggtcaagctg cgtcaggggc
     5701 tgaacgacga tctggcccgc gagatcagca agcgcatccg gactgagttc aagaaggtca
     5761 cggcgcaaat ccagggcgac ctgattcggg tgtccgccaa gaatcgggac gacctgcaga
     5821 gcgtcattca ggcgttgaag gagtccgatt acccggtgcc gctccagttc gtcaactatc
     5881 gctagtcgtc ggattcggcg gcggcgccct gcggagcggt atcagcgttg gtggcggaag
     5941 cttgttcgtc ggccgccgcc tgccgggcgg ccgatgcggc tgcgaggcga tccagcaacg
     6001 cgcggcgccg cggttgctcg tcatcccaga ctcgctggaa catgacccac tggtcgggcg
     6061 ccatcgagat gccctgctcc aggacagcga ccatcttcgc gagaccggcg ctgacgtcag
     6121 cgtcctcatc ggccgtccgc tcgacgtaga ccggatccag caagtgaaac gcgtagcgtt
     6181 gccgccggcc gccgtcgtcc tctcggatgg cgaagacagg caggatcggg gcgccggaga
     6241 cccgcgcgag ccggaccggg cctaacggca gcgttgtctc aacgccgaaa aacgtcgctg
     6301 gcccgccaga gtcggtgaag tcgcgatcgg cgacgacccc cgcaaacccg cccttcagca
     6361 ggacacgcag catccgccga atcgcggccg gcgagatatc ttcgagcggc gcgttctggc
     6421 tgtggcgcag gtagttgacg gcggcgtaga cgaactcacc tacggtcggc gacgtcagcg
     6481 tgtaggggtt gtagccgcgc atgaacagcg tctgaccgac gaagtcgaac gcgccgagat
     6541 gggcggtcat gatgatgccg cctttgccct cctcgcggat gcgatcgaac aggctccagt
     6601 cgttggcagg cagccggacc atccgctcga agtcgcgaac ggtcagatgc ggcacgcggg
     6661 taaggtccca gaaattgcga gccgaggtgc ggaagacacg tcgcgcatag cgccggagca
     6721 tcaactcacc gatcgagggc ccatagacat ggcgcagatt ttcgatgacg ttgatccggt
     6781 agcgccgcga gcgccagtag agcagatcgc cgacccggtc gcagaccatg tagccgacac
     6841 gcagcgggat gacccgcgtg atatgcgacg caacgcgagc ggcgaagcgt cccagccgct
     6901 ggcgcatcgt tgggcgtcgc gtcggaatag ccgcccgcgc ggaatcaggc cggctcgcca
     6961 cgttcggtcc gctccgtcga caacagcgtg cccggttcgc gtccagccgg gtatggttct
     7021 tccgcctcgc gcaggtagta cctcgcgtcc cagatcaccg gcaagccacg gatgacacag
     7081 agcgcgaccg aggcatagac cagcgtccag ccgatcccgc gtacgatctg atagtcgtag
     7141 agcgtatcca gaatgcgccc atcggcatcc ggcagtcgcc atgcaaccag ccaggtcagg
     7201 aagacgaagc cgatcgcctt cgaccagccg taggcggtcc ggctgaagcg ggaggcagtc
     7261 aggaaccagg tcagccgcga gcgggccatc gtcgtctcgc caaacggcgt cttgccctcc
     7321 gtgagcgcga ccgaacgcag cacatcgacg acgaacgagc gcgtcaggac aatcaatggc
     7381 gcccagacgc cgacgatctg catttcagcg aagacaaccc agagaacgtt ttcgacgacg
     7441 cggtcgccgg cgatgtcgag gaccgcgccg agcttgctgg aggagttgcg gcggcgtgcg
     7501 accccgccgt cgagcccgtc accggcaaag atcgcgccga tcaaaacgcc ggcgacgacg
     7561 atcgtccagt agttaccgga atagaccatc gcgatcgcga cgaagagcaa cacgacgcgt
     7621 ccgacggtga tgctattggc catcagatct ccgccatcat ccccgcctca accgaatacc
     7681 acgcttcggg cagcccgcgt cgccagtata gcagccgacc ggcgctcaac agccatcctg
     7741 ctgctggtag tcatttcggg gcggcgaaaa cccctcccct aaagatggag gggctttacg
     7801 ccgcacgaaa tctaaagccc ctctccctgt ggagggggaa tggggaggga ttttcaactc
     7861 gccgcacgaa ccagcgcgat gaagaggatc aactggacaa ggggaatgcc actgagcagc
     7921 aggcgggcga ggtagcggtc gaggtcaatc agcagcgtcg ccagggccgt gttgacgatc
     7981 aggacgacga cggcgatgcg tgggaagcgc cagagcccgc tcggcgcggt ctggccgatg
     8041 acgtccccgg tggcgtccca gcggatcggg atctggtccg gcaggccctc gtactgactg
     8101 tagagataag cgaccatcag caccggcagg acgacaccgg ccgcgacgag cgccgacgcg
     8161 atctgatctc cgagcag
//
LOCUS       DASUGJ010000456         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1739513, whole genome shotgun sequence.
ACCESSION   DASUGJ010000456 DASUGJ010000000
VERSION     DASUGJ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739513"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2573
                     /gene="carB"
                     /locus_tag="VFV93_10000"
     CDS             <1..2573
                     /gene="carB"
                     /locus_tag="VFV93_10000"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5165717.1"
                     /translation="RDGADNVITVCNMENLDPMGVHTGDSIVVAPSQTLSDREYQMLR
                     SSAIKIIRALGIEGGCNVQYALDPDSFQYFVIEVNPRVSRSSALASKATGYPIARVAA
                     KIAIGKTLDQIRNSVTEQTTAAFEPALDYCVVKVPRWPFDKFRRADRSLGTQMKATGE
                     VMAIDRSFAAAMQKAVRSLETDAPDLLWENSAWSDPERLALDVRVASDERLWALATSL
                     RRGADEETISRESGVDRWFIRELAQIVAMEQLLAEGEIDDATLWRAKRLGFSDAQIAS
                     LSGRDETDVRQQRYTAGITPVYKMVDTCAAEFEAATPYYYSTYDEEDEAAPLQGPKAV
                     VIGSGPIRIGQGIEFDYCSVQAAHALGAAGTRSIMINSNPETVSTDFDTSDRLYFEAL
                     DPESVGEILRHEGRGADDQVPVILQFGGQTAINLASPLSARGVPVLGSSVDTIDLAED
                     RERFEAFLTRIGIPQPPGTAVTTLDAALEAADRLGYPVLVRPSYVLGGRAMEVVHSAD
                     FLARYLATSVDFRRGFPILIDRYLEGKEIEVDAICDGSEVLIPGIMEHIERAGVHSGD
                     SYAVYPGVNLFPSEVERIVEYTTRIALELKVIGLVNIQYVIHSGRLYVLEVNPRGSRT
                     VPFLSKATGVPIIEIATHAMLGKSLAEQGYSGGLAARQPLVAVKAPVFSMAKLRGVEP
                     NLGPEMKSTGEAMGIDREFAPAFYKALLASGLSLNGRASIMVSLADRDKPESLTMIEQ
                     LIRMGCRVYATEGTAQMIERAGMPVQMVTKRIGQGSPDMLDIILDGTVDGVINTPGPA
                     EKEVLDGFQIRRAAVEKGIPCITSIDTARAVVEAMRQSTGAYSVQPLPSYRNRVSVSY
                     "
BASE COUNT          471 a          888 c          870 g          461 t
ORIGIN      
        1 tccgcgacgg tgccgacaac gtcatcaccg tctgcaatat ggagaacctt gacccgatgg
       61 gtgtccacac cggcgactca atcgtcgtcg cgccgagcca gacgctctcc gaccgcgaat
      121 accagatgct gcgctcatcc gcgatcaaga tcattcgcgc gctgggcatc gagggcggct
      181 gcaacgtcca gtacgcgctc gatccggact cgttccagta cttcgtgatc gaggtcaacc
      241 cgcgtgtcag ccgctcgtcg gcactggcgt cgaaggcaac cggctacccg atcgcccgcg
      301 tcgccgccaa gatcgcgatc ggcaaaacac tggaccagat tcgcaactcg gtcaccgagc
      361 agacgacggc tgccttcgag ccggcgctcg actactgcgt cgtcaaggtg ccgcgctggc
      421 cgttcgacaa gttccgccgt gccgaccgct cgctcggcac ccagatgaag gcgaccggcg
      481 aggtgatggc gatcgaccgc tcgttcgccg ccgccatgca gaaggcagtt cgctcgctgg
      541 agacggacgc gcccgatctg ctctgggaga attcggcctg gtccgaccct gagcgactgg
      601 cgctcgacgt ccgtgtcgcc agcgacgaac ggctctgggc gctggcgacc agtctgcggc
      661 gcggggcaga cgaagagaca atctcccgcg aaagcggcgt cgatcgctgg ttcattcgcg
      721 aactggccca gatcgtcgcg atggagcagc tactcgccga aggcgaaatt gacgacgcca
      781 cgctctggcg ggccaagcga ctcggtttct cggacgcaca gatcgcgtca ctctccggtc
      841 gcgatgagac tgatgtccgc cagcagcgct acacggccgg gatcacaccg gtctacaaga
      901 tggtcgatac ctgcgctgcc gagttcgagg cggcgacgcc gtactactac agcacctacg
      961 acgaggagga cgaagccgcg ccgctccaag gtccaaaggc ggtcgtcatc ggctccggtc
     1021 cgatccggat cgggcagggg atcgagttcg actattgcag cgtccaggcg gcccatgcgc
     1081 tcggcgcggc cggcacacgc agcatcatga tcaactccaa ccccgagacg gtctcgaccg
     1141 acttcgacac ctccgaccgg ctctacttcg aagcgctcga tcctgaatca gtcggcgaga
     1201 tcctgcgcca cgagggccgc ggggccgacg accaggtgcc cgtcatcctc cagttcggcg
     1261 gccagacggc catcaacctg gccagcccgc tttccgcacg gggtgtgccg gtgctcggct
     1321 ccagcgtcga cacgatcgac ctggccgagg accgcgagcg attcgaagcc ttcctgacgc
     1381 ggatcggcat cccgcagccg ccgggtacgg ccgtgacaac gctggacgcc gcacttgaag
     1441 cggccgaccg gcttggttac ccggtcctcg tccgcccgtc gtatgtcctc ggtggccggg
     1501 cgatggaggt cgtccactcg gctgacttcc tggcgcgcta tctggcgacc agcgtcgatt
     1561 tccggcgcgg cttcccgatc ctgatcgacc gctacctgga gggcaaggag atcgaggtcg
     1621 acgcgatctg cgacggcagc gaggtgctga tccccggcat catggagcac atcgagcggg
     1681 ccggggttca ctccggtgac tcgtacgccg tctacccggg cgtcaatcta tttccgtccg
     1741 aagtcgagcg catcgtcgag tacacgacgc ggatcgcgct tgagctgaag gtcatcggtc
     1801 tcgtcaacat ccagtatgtc atccactcag ggcggctcta cgtccttgag gtcaacccgc
     1861 gcggtagccg gacggtgccg ttcctctcga aggcgaccgg cgtcccgatc atcgagatcg
     1921 cgacccatgc gatgctgggc aagtcgctgg ccgagcaggg ttacagcggc ggactggctg
     1981 cccgccagcc attggtcgca gtgaaggcgc ccgtcttctc gatggcgaag ctgcgtggtg
     2041 tcgagccgaa cctcggcccg gagatgaaat caaccggcga ggcgatgggg atcgatcgcg
     2101 aattcgctcc ggccttctac aaggcgctgc tggcgtcggg gttgtccctc aatggccgcg
     2161 cgtcgatcat ggtcagcttg gctgaccgcg acaagccgga gtcgctgacg atgatcgagc
     2221 agttgatccg catgggctgc cgcgtctacg cgaccgaggg cacggcccag atgatcgagc
     2281 gggccgggat gccggtccag atggtgacca agcggatcgg gcaaggctcg ccggacatgc
     2341 ttgacatcat cctcgacgga accgtcgacg gagttatcaa cacgcctggg ccggctgaga
     2401 aggaagtgct ggacggcttc cagattcgtc gcgcagccgt cgagaagggg ataccctgca
     2461 tcacctcgat cgacacggcc cgggccgtcg tcgaggcgat gcgccagtcg accggggcat
     2521 attccgtgca accgctgccg tcgtatcgga atcgtgtgtc ggtatcctac tgacctgctg
     2581 agtcggcgtc cggcaccaga tcactgagtc gctgaaccca ttcagaggga gggctgatgc
     2641 cacgttggtt gtacgggttt ctggtcttcg gcgttgccgc gctggtcgcg
//
LOCUS       DASUGJ010000457        16703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1044325, whole genome shotgun sequence.
ACCESSION   DASUGJ010000457 DASUGJ010000000
VERSION     DASUGJ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 16703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16703
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044325"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..2462
                     /locus_tag="VFV93_10005"
     CDS             333..2462
                     /locus_tag="VFV93_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX5165718.1"
                     /translation="MNYDQSAVRTILDRVRDDGRTALTAEEARSVCTAYGITMPGEGL
                     ARTQDDAVKLARDLGHPVVLKIASPDILHKTDAGGVETGLDSDEAVGRAYERIIANAR
                     AYKADANVLGVQVQQMVPAAHEVIIGAVTDPSFGKLVAVGLGGVLVEVMRDVTFRLAP
                     VTRDEALSMFGDLRGGAVLSGVRGQAGANLDKLASLVEGVSQLVGDFPEIHELDLNPV
                     FASESGAIAVDARILADFAPAEPRYRPSQEEILRAMRRIMQPKSIAVVGASPEDGKIG
                     NSVMKNLINGGYGGEIYPIHPRAEEILGRTCYKSVLDVPGEIDVAVFCIPAKLVAGVL
                     EEIGQKEIPGAVLIPSGFAEVGEVELQNEVLAVARKHNIRLMGPNIYGFYYTPMDLCA
                     TFCTPYTERGEVALSSQSGGVGMAIIGFSRSNQMGISAIVGLGNKADLDEDDLLTFFE
                     QDDNTQVIAMHVEDLKDGRSFAEVAQRVSKQKPVVVLKAGRTSMGARAARSHTGALAG
                     NDKIYDDVLRQSGVIRALSLNDMLEFARGLQVLPTPAGENVLIITGAGGSGVLLSDAC
                     VDNGLQLMPMPDDLDAAFRQYIPPFGASGNPVDITGGEPPTTYRSTIRLALDDERVHA
                     LILGYWHTIITPPMTFANLLSEEIEAARERGIDKPVVASLVGDIEVEDACRHLMGHNI
                     LAYPYTTEKPVAVLGAKYRWARQAGQI"
     gene            2694..3386
                     /locus_tag="VFV93_10010"
     CDS             2694..3386
                     /locus_tag="VFV93_10010"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5165719.1"
                     /translation="MSVVRLTDPPLDFAFSLKDRVYDALKESIAGMNIYEGTEELRLD
                     ERQLSEDLGVSRTPVREAIARLEQEGLVRIVPRRGVFVARKTKREIVEMITVWAALES
                     MAARLITKQATDDEIAGLRALVATIKDEIAQAHIDEYSSMNIRFHQAIIRLSKSQLIE
                     SMTETLFIHMRAIRARTITENNRVQRSIIDHMNIINALEARDADLAERLSREHTLGLA
                     AHVEQYVTYLDQ"
     gene            3461..5200
                     /gene="oxc"
                     /locus_tag="VFV93_10015"
     CDS             3461..5200
                     /gene="oxc"
                     /locus_tag="VFV93_10015"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF006721.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEX5165720.1"
                     /translation="MADIETQVGTDAPEEAGALTDGFHLVIDALKLNDINTIYNVPGI
                     PITDLGRFMQDAGMRVLSFRHEQSAGYAAAIAGYLTKKPGVCMTVSAPGFLNGLTALA
                     HATTNCFPMILISGSSEREIIDLQQGDYEEMDQLAVAKPLCKAAYRILNAEDIGIGVA
                     RAIRTAVSGRPGGVYLDLPGKLLGQTIDAAIGASTLVKVVDAAPRQLPAPEAVDRAIE
                     VLKSAKRPLIILGKGAAYAQADAEICELVETSGIPYLPMSMAKGLLPDTHPQSASAAR
                     SLVLQQSDVVLLIGARLNWLLSHGKGRTWGNRPRKFVQIDIEPREMDSNVGIDAPLVG
                     DIGSCVSALLERMRAGWTPAPVEWTAEVGDKVESNVARMAPRLRNSNVPMDFHGALGA
                     LRTVIKDRPDTILVNEGANTLDFARSIIDMYQPRKRLDVGTWGVMGVGMGYAIAAAVE
                     TGNPVLAVEGDSAFGFSGMEIETICRYNLPVCVVVFNNGGIYRGTDVNPSGGPDVATT
                     VFVKGARYDKMMEAFGGVGVHVTTPDELSRAVNAAIDSGKPTLVNAVIDEGAGTESGR
                     IGNLNPQSVVARR"
     gene            5244..6494
                     /gene="frc"
                     /locus_tag="VFV93_10020"
     CDS             5244..6494
                     /gene="frc"
                     /locus_tag="VFV93_10020"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF003809.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEX5165721.1"
                     /translation="MGKALEGVRILDMTHVQSGPTCTQLLAWFGADVIKVERPGAGDA
                     TRSQLRDIPDVDSLYFTMLNHNKRSVTLNTKTKAGNRIFARLIEECDVLVENFAPGVI
                     DRMGFPWEKIQEINPRMIYASIKGFGPGPYDDCKVYENIAQSTGGAASTTGFLDGPPT
                     VTGAQIGDSGTGLHLAFGIVTALFQREQTGKGQRVEVAMQDAVLNLCRVKMRDQQRLA
                     HGPLREYSQFGQGIPFGEATPRAGNDSGGGQPGWIVKCKGWETDPNAYVYFITQAAVW
                     GNICDLIGKPDWKEDPNYATPDARLDKIPQIFDTIEEWTKTLDKFEVMDLCNPLNIPC
                     GPILSMKELAEEPSLRATGTIVEVDHPTRGKYITVGNPVKLSASPSDVKRSPLLGEHT
                     DEILRTVVGLGEDDIAAAREEGAI"
     gene            6842..7246
                     /locus_tag="VFV93_10025"
     CDS             6842..7246
                     /locus_tag="VFV93_10025"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX5165722.1"
                     /translation="MSADRREVPLLRLSEDEFEELVVAALDTLPEDLLELMDNIEVTV
                     ERAPSRQQMRRVGLRSDTLLGFYEGIPLTARNSSSYSLVVPDKITIFQRPIEGICSTH
                     DEVVAQVRKTVIHEVAHHFGIGEERLRELGWG"
     gene            7261..8268
                     /locus_tag="VFV93_10030"
     CDS             7261..8268
                     /locus_tag="VFV93_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165723.1"
                     /translation="MHDWGREDEAGSTRDGRIDRDAPLPGVIDTIGFGYAALALRPLA
                     ILPLVALELYLLLGPKVALAPLTNGLADLLRRRGSHWDDLAGEIGRLEGFNIFEVGSL
                     EIPLLRTPGIVPTFSGDEIANLRIGANWSSLPWGVVVLLLIASLIVGLLLAVAYRSLL
                     AQAALATTNQSIAIGPKAIARRAWRIAGWGLALAGLALLVAMPVLVATIAGAVIGLGE
                     LTLLWLFLLFPATWAWVHFYFSVHALVITDAGPLESLRASYQVVQHHFWQSLRFMAAS
                     LLITTGLSFALRELATSLPGALLAILLNAVIASGMIVAAMLFYRDRADRLGLHVQVPG
                     R"
     gene            8288..10213
                     /gene="gyrB"
                     /locus_tag="VFV93_10035"
     CDS             8288..10213
                     /gene="gyrB"
                     /locus_tag="VFV93_10035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX5165724.1"
                     /translation="MKTNPTAPARKSYDAKDIEVLEGLEAVRRRPGMYIGSTDLRGLH
                     HLITEVVDNAIDEAIGGYCDTVRITIHPDASVTVHDNGRGIPTGKHPKLNTSALEVVM
                     TILHAGGKFGGDGYKMSSGLHGVGVSVVNGLSDWMVSEVRRDGKLHRQEYRRGVPTGP
                     VRKVGSVHPDDHGTTQTWMPDATIFDTLDYNFEALAQRFREMAYLTKGLRIVFTDERS
                     DREMTFYFEGGIQSYVRHLNKTRNVLHPKPFYMHRQVGDFIVEVALQYNDTYGESTHT
                     FANNINTVDGGTHLTGFKSALTRTINDYARKNGFLKENDDSLSGEDVREGLTAIVSVK
                     LPEPQFEGQTKGKLGNAPMAGAVQSVVNELLAQHLEENPQAAKRIVDKCITAARARDA
                     ARKARDLVQRKGSLDTFSLPGKLADCSERDPARAELYIVEGDSAGGSAKQGRDRRFQA
                     ILPLRGKILNVEKARLDKMLQNAEIRALITALGTGIGDGFDASKLRYHRIIIMTDADV
                     DGAHIRTLLLTFFFRHMESLIVDGRLYIAQPPLYRLQKGNDVRWVYNDEERDRVMKEF
                     KDGRVEIQRYKGLGEMNPEQLWETTMDTASRTLLQVTIEDAVHSDETFDMLMGNAVPP
                     RKRFIQTHAREVQNLDV"
     gene            10360..11397
                     /gene="plsX"
                     /locus_tag="VFV93_10040"
     CDS             10360..11397
                     /gene="plsX"
                     /locus_tag="VFV93_10040"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX5165725.1"
                     /translation="MRIALDTHGGDFGVPRNISAALDIATGSDTNLVLVGHRNEIEAE
                     LRRCNTPVNAFEIVHAEEQIGMGEAAVQAVRRKADNSITVALGLMKSGAVDAVVSAGH
                     SGAVVAAALMTLGRIRGVSRPPLGTRIPAINTHNSFALDIGAVIDPKPEMLLQFGYLG
                     AAYSKAVLDVPNPSVALLSNGEESSKGNALTRAAQELFERSDLNYIGYVEGGEMLDKP
                     PAVTVTDGFTGNIGLKIAEGTAASIQHALRRELTANWRDKLLASLLRPAFRRMRDNMD
                     FEGIGGAALLGVNGVVIVAHGRSTTRGIVSAIRTAEHAAAVRLPELLRDAIPVRDQRL
                     AEPSDTTATVS"
     gene            complement(11572..12165)
                     /locus_tag="VFV93_10045"
     CDS             complement(11572..12165)
                     /locus_tag="VFV93_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165726.1"
                     /translation="MNGHVPQRIIVIGSSNAGKTTLATRLAGILGAPMIELDALHWES
                     GWREAELAVFQDRVRTATDSQRWVMDGNYLPKQQDVSWPRADTIVWIDLPLATLLRRC
                     VGRSWSRWRSQEQLYGGDNRERFVEHLMLWDTDRSLISHIIRTHRRRRRDYSAMSRDP
                     QWAHLTVICLRSTDEIETWLQDLGHIAEQSEDPSIRA"
     gene            complement(12254..12538)
                     /locus_tag="VFV93_10050"
     CDS             complement(12254..12538)
                     /locus_tag="VFV93_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5165727.1"
                     /translation="MRKQDLVKAVATSTNMPEGQVSNIVQATFDAIRDQLAAGEEVTI
                     TGFGSFRISERGAREGRNPQTGERITIAARKSPSFRPGTQLKRAVGGDDA"
     gene            complement(12665..13483)
                     /locus_tag="VFV93_10055"
     CDS             complement(12665..13483)
                     /locus_tag="VFV93_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5165728.1"
                     /translation="MGDDRVHRQAELITSPANPTIKLARSLHRKRVRYRERALLVEGV
                     RSIRTASDLGVQIRAFMIDASRQDELDPALVATLSALASRTLLIDAPLFKQITDTEHP
                     QPLAAICAMPESVLPVDCSFVVALDGVRDPGNLGTLVRSSAAAGADGVALLPGSADPF
                     NPKAVRASVGSIFALPVQSFSSIFEITSGSFISLPSVIIADSDGKRAYDDVDWTAPVL
                     LIIGGEAEGAGQEARTYADELVSIPMAPGVESLNAAVAGSIIAFEVARQRRRRS"
     gene            complement(13494..13847)
                     /gene="rplT"
                     /locus_tag="VFV93_10060"
     CDS             complement(13494..13847)
                     /gene="rplT"
                     /locus_tag="VFV93_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5165729.1"
                     /translation="MARIKRGVTTRRRHRAVLNRAEGFRGTRNRLFKRANEAVMRSLM
                     YQYRDRRTRKRQMRRLWITRINAAARANGMTYGRFIEGLHKAGVTVDRKILADLAVRD
                     AAAFSKFVEVARGTS"
     gene            complement(13904..14107)
                     /gene="rpmI"
                     /locus_tag="VFV93_10065"
     CDS             complement(13904..14107)
                     /gene="rpmI"
                     /locus_tag="VFV93_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5165730.1"
                     /translation="MPKMKTNKQAARRFKITGTGKVMRAKGGKSHFRRRKSPRTRRMF
                     DKMFEVNAADKKRIKRLLPYGTE"
     gene            complement(14208..14816)
                     /gene="infC"
                     /locus_tag="VFV93_10070"
     CDS             complement(14208..14816)
                     /gene="infC"
                     /locus_tag="VFV93_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5165731.1"
                     /translation="MSTRPVRVNERIRVREVRLIDEDGTQLGVVQTRDALEIARERGL
                     DLVEVQPNAVPPVCRLMDYGRFRYEESRKERESRKKSKGAVLKEVRIYPKIGDHDLHT
                     KARQAQRFLEDGDKVKVAVLFRGREMLHQEIGRELLKQVVAQLQPVSTVDQDARMEGR
                     TMSVLLSPKKEFVKVDGVSPADVEKARLAQGEPPQPEAQTKG"
     gene            complement(14925..>16703)
                     /locus_tag="VFV93_10075"
     CDS             complement(14925..>16703)
                     /locus_tag="VFV93_10075"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX5165732.1"
                     /translation="IVKLSTIPTATGFNLALATLFASGATVAAAAGVTLLRALGSGRR
                     LVIVGAALSVVLLLLGANLETARRMIDDPGATIDAGWWDGVGWQASRIIYDNDVNEPG
                     DEKQTINEFPAFSYVLGDLHPHVLTYPLLAAVLVLALSLALFREPEPWTRLAAFGALV
                     GLLYASNSWDAPAGMLLVAGGLALKHRYLWRPIVRDLLIVAGGALLMAGPFMLEFSAP
                     VGVPNASVPAWLADLPVVGRAFDTFAIVSWRPSSVRELLIVHGAWLAAFAGLAAIELV
                     RDRALLMIFRRRPQIWLAVAILAFGVAMAWAPAVLLLGVPLALALWFAAHSDRQPARV
                     LAGLFAAGFVLALIPEFVYIQDVFNDRMNTVFKLFYQAWLLLSIASAATIVYVATQIP
                     RAYRVPAFAALGLLVLLTLPYTPLSAQDWTNDFATRYGLNGSAYLSRSAPGDLAAIDW
                     IAESSEPGDTIVEQPGCSYINAAGAPLSRISAFSGVPTILGWHGHESQWRRGEYAAIR
                     DILDLRAAQANAIIDGTVTADETDARFVILGRQEIQGTTDCNLTRQRTGDPVATLRAA
                     GWEIAFVSEQTTIFAHPGDPILVGED"
BASE COUNT         3139 a         5237 c         5467 g         2860 t
ORIGIN      
        1 tccatgcctg tgtcacgagg atccccatcg cctgcacccg tggatggacc aggccggtgc
       61 cacgcgggcg cctcacggcg cggacagatc tgagacctgt ccctacgcga cacacccact
      121 aaggtggacc actgcggcgc aggacgacag gtgtacgccg aaaattgcgt gctttgagcg
      181 gctgcactaa gatgctcgat gactgatccg ggggacgggg cagcgaggca ttgcgacggt
      241 cttcaggaca ttgtcctacc tcatccaacc agcgccctcc gaacgacaac ttcgcgaaca
      301 gcacgccagt atctcgcgtg gaaggaatcc gcatgaacta cgaccagagc gccgtccgca
      361 ccatcctcga tcgggtccgt gacgatggac ggacagcgct gacggcggag gaggcccgca
      421 gcgtctgcac ggcctacggg atcacgatgc ccggtgaggg tctggcgcgg acgcaggacg
      481 acgctgtcaa gctggcgcgc gacctcggtc atccggtcgt gctcaagatc gcgtcaccgg
      541 acatcctgca caagaccgat gccggtggcg ttgagaccgg tctcgacagc gacgaagcgg
      601 tcgggcgagc atacgagcgg atcatcgcga acgcgcgagc atacaaggct gacgcgaacg
      661 tgctcggtgt ccaggtgcag cagatggtac cggcggccca cgaggtcatc atcggcgccg
      721 tcactgatcc gagcttcggc aagctggtcg cggtcggtct cggcggcgta ctggtcgaag
      781 tgatgcgcga tgtcaccttc cggttggcgc cggtcacgcg cgacgaggcg ctgtcgatgt
      841 tcggcgacct gcgcggcgga gccgtcctga gcggcgtacg cggacaggcc ggcgcgaacc
      901 tggacaagct ggccagtctg gtcgagggcg tttcacaact ggtcggggac tttcctgaga
      961 ttcacgagtt ggacctgaat ccggtcttcg cgagcgagtc gggcgccatc gcggtggacg
     1021 cgcggatcct ggccgacttc gcaccggccg agccgcgcta ccgaccctcg caggaggaga
     1081 tcctgcgcgc catgcgccgc atcatgcagc cgaagtcgat tgccgtcgtc ggcgcgtcac
     1141 cggaggacgg caagatcggc aactcggtca tgaagaacct gatcaacggc ggctacggcg
     1201 gtgagatcta cccgattcac ccgcgggcgg aagagatcct cggccgcacg tgctacaaga
     1261 gcgtcctgga tgtgcccggc gagatcgacg tcgccgtctt ctgcatcccg gccaagctgg
     1321 tggccggcgt cttggaggaa atcggccaga aggagattcc cggcgcggtc ctgatcccat
     1381 ccggtttcgc cgaggttggc gaggtcgagc tgcagaacga agtgctcgcc gtcgctcgca
     1441 agcacaacat ccggctgatg ggtccaaaca tctacggctt ctactacacg ccgatggacc
     1501 tctgcgcgac gttctgcacg ccttacaccg aacgcggcga ggtggcgttg tcgtcgcaga
     1561 gcggcggcgt cgggatggcg atcatcggct tcagccgctc gaaccagatg ggcatctcgg
     1621 ccattgtcgg gctcggcaac aaggccgacc tcgacgagga cgacctgctg acgttcttcg
     1681 agcaggacga caacacgcag gtgatcgcga tgcacgtcga ggatctgaag gatggacgct
     1741 cgttcgccga ggtcgcccag cgcgtctcga agcagaagcc ggtcgtcgtc ctcaaggccg
     1801 gccgtacgtc gatgggtgcg cgggcggccc ggtcgcatac gggggcgttg gccggcaacg
     1861 acaagatcta cgacgatgtc ctgcggcaga gcggcgtcat tcgcgccctg agtctcaacg
     1921 acatgctgga gttcgcgcgg ggcttgcagg tgctgccaac tccggccggc gagaacgtgc
     1981 tgatcatcac cggcgcaggt ggctcgggcg tgctgttgtc cgacgcctgc gtcgacaacg
     2041 ggctacaact gatgccgatg ccggatgatc tcgacgctgc cttccggcag tacatcccgc
     2101 cgttcggtgc gtctggcaac ccggtcgaca tcaccggcgg cgagccaccg acgacctacc
     2161 gctcgacgat ccggctggcg ctggacgacg agcgcgtcca cgccctgatc ctcggctact
     2221 ggcacacgat catcacgccg ccgatgacgt tcgcgaacct gctcagcgag gagatcgaag
     2281 cggcccgcga gcgcggcatc gacaagccgg tcgtcgcctc gttggtcggc gacatcgagg
     2341 tcgaggatgc ctgccggcac ctgatggggc acaacatcct ggcctatccg tacacgaccg
     2401 agaagccggt ggcggtcctg ggcgccaagt atcgctgggc acgccaggca gggcagatct
     2461 agaggggcac gtcgagccga agaccgctgg ccgtcatcga gattgacgac ggccagcggt
     2521 cgtctatcgc atgaactcgg tcgacgcggc gctgcaaacg aactcagatc gaaagacgcg
     2581 aaggcagtga atttcggcgg tcttctactg cggtgtgcgg tatgtataat gtataccacg
     2641 ctccgttgtg acgctctcaa catggtttct gatcgtatgg caggacgttc acagtgtcgg
     2701 tcgttcggct cacggatccg ccgcttgatt tcgccttcag tctcaaagac cgtgtctacg
     2761 acgcgctgaa ggaatcgatc gccggcatga acatctacga gggtacggaa gagctacgcc
     2821 tggatgagcg gcagctttcc gaggatctgg gcgtcagccg gacgccggtt cgagaggcca
     2881 tcgcgcggct cgagcaggag ggcctcgtcc ggatcgtgcc gcggcgcgga gtcttcgtcg
     2941 ctcgcaagac caagcgcgag atcgtcgaga tgatcaccgt ctgggcggct cttgagagca
     3001 tggccgcacg gctgatcacc aagcaggcga ccgacgacga gatcgccggt cttcgagcgc
     3061 tcgttgcgac gatcaaagac gaaatcgccc aagcccacat cgatgagtac tcgtcgatga
     3121 atatccgctt ccaccaggcc atcatccgac tcagcaagtc ccaattgatc gagtcgatga
     3181 cggagacact cttcatccat atgcgcgcga tccgggcgcg gaccatcact gagaacaacc
     3241 gtgtccagcg ctcgatcatc gatcacatga acatcatcaa cgcgctcgaa gcgcgtgatg
     3301 ccgacctggc cgagcgcctg tcacgcgagc acacgctggg gctggcggcg cacgtcgagc
     3361 aatacgtgac ctatctggac caatgatcgg tcgacggtaa ttagacgacg catgcgagcg
     3421 cacgggcact ctgtccggca acgatcggga ggagcgggga gtggcagata tcgaaacgca
     3481 ggttggaacc gacgcgccag aagaggcagg cgcgctgacg gacggatttc accttgtcat
     3541 cgacgcgctc aagctcaacg acatcaacac gatctacaac gtgccgggca tcccgatcac
     3601 cgatctcggc cggttcatgc aggacgccgg aatgcgcgtt ctctcgttcc gacacgagca
     3661 gagcgcgggc tatgcggcgg cgattgccgg gtatttgacg aagaagcccg gcgtttgcat
     3721 gaccgtctcg gcgcctggct tcctgaatgg gctgacggcg ttggcccacg cgacgaccaa
     3781 ctgcttcccg atgatcctga tcagcggctc gtccgagcgt gagatcatcg acttgcagca
     3841 gggggactac gaagagatgg accagctcgc cgtcgcgaaa ccgctctgca aggcagccta
     3901 ccgcatcctc aacgccgagg acatcggtat cggggttgcc cgcgcgattc ggacagccgt
     3961 gtccggccgg ccgggaggcg tctatctcga tctgccgggg aagctcctcg gccagacgat
     4021 cgatgcagcc atcggggcca gcacgctcgt caaggttgtc gatgccgcgc cgcgccagct
     4081 gcctgcgccg gaggcggtcg accgagcgat cgaggtgctc aagagcgcca aacggccgct
     4141 gatcattctc ggcaaggggg cggcttacgc gcaggccgat gccgagatct gcgaacttgt
     4201 cgaaacgagc ggcattccct acctgccgat gagcatggcc aaggggctgt tgccggacac
     4261 ccatccgcag tcggcatcgg ccgcccgctc gttggtcctg cagcaatccg atgtcgtgct
     4321 gctgatcgga gcccggctga actggctact gtcgcacggc aaaggcagga cgtggggcaa
     4381 tcggccgagg aagttcgtgc agatcgacat cgagccccgc gagatggaca gcaacgtcgg
     4441 gatcgacgct ccgctggtgg gcgatatcgg ctcctgcgtg tcggcgctcc tggagcgcat
     4501 gcgtgcgggc tggactcctg cgccggtcga gtggactgcc gaggtcggcg acaaggtcga
     4561 gagcaacgtc gcccggatgg cgccccggct ccgcaactcc aatgtgccga tggactttca
     4621 tggagcgctc ggcgcgctgc gcaccgtcat caaggatcgg cccgacacga tcctcgtcaa
     4681 cgagggcgcc aacacgctcg atttcgcacg cagcatcatc gacatgtacc agccccgcaa
     4741 acggctggac gtcggcacct ggggtgtgat gggggttggg atggggtacg cgatcgcggc
     4801 tgccgtcgag accggcaatc cggtcctggc cgtcgagggc gacagcgcgt tcggcttctc
     4861 cgggatggag atcgaaacca tctgccgcta caacctgccg gtctgcgtcg tcgtcttcaa
     4921 caatggcggc atctatcggg gcaccgatgt caacccatcc ggtggcccag acgtggcaac
     4981 gaccgtcttc gtcaagggcg cccgctacga caagatgatg gaggcgttcg gcggtgtcgg
     5041 cgtccacgtg acgacacccg acgagttgag ccgcgcggtg aacgcggcca tcgattccgg
     5101 caagccgacg ctggtgaacg cggtcattga tgagggggcc gggaccgaga gcggtcgcat
     5161 cggcaacctc aatccacaga gcgttgtggc cagacgataa ccaagtggca ttcgtacagg
     5221 cagagagaag gagagagggg attatgggca aagcgctgga aggcgtccgt attctggaca
     5281 tgacccacgt acaatccggc ccgacctgca cgcagttgct ggcgtggttc ggcgcggacg
     5341 tcatcaaggt cgaacgaccc ggcgcgggtg acgctacgcg cagtcaactc cgtgacattc
     5401 cagacgtgga tagcctctac ttcacaatgc tgaaccacaa caagcgcagc gtgacgctga
     5461 ataccaagac caaagccggc aacaggatct tcgcccggct catcgaggag tgcgatgtgc
     5521 tggtggagaa cttcgccccc ggcgtcatcg accggatggg ctttccgtgg gagaagattc
     5581 aggaaatcaa cccgcgcatg atctacgcgt cgatcaaggg cttcggcccc ggcccctacg
     5641 acgactgcaa ggtctatgag aacatcgccc agagcaccgg cggggcggcc tcgacaactg
     5701 gctttctcga tggccctccg accgtgactg gcgcgcagat cggcgactcg ggcacgggat
     5761 tgcatctggc gttcgggatc gtgactgcgc tgttccaacg cgagcagacc ggcaaaggcc
     5821 aacgcgttga agtcgccatg caggacgccg tcctgaacct gtgccgggtg aagatgcgtg
     5881 accagcagcg gctggcccat ggcccgctgc gggagtacag ccagttcgga caggggattc
     5941 cgttcggtga agcgacgccg cgtgccggca acgactccgg cggcggtcag ccgggctgga
     6001 tcgtcaagtg caagggttgg gaaactgacc ccaacgcgta cgtctacttc atcacgcagg
     6061 cagccgtctg gggcaacatc tgcgacctga tcggcaagcc cgactggaaa gaagatccca
     6121 actacgccac gcccgacgcg cggctcgaca agatcccgca gatcttcgac acgatcgagg
     6181 aatggacgaa gacgctcgac aagttcgagg tcatggatct gtgcaacccg ctcaacatcc
     6241 cctgcgggcc gatcctgtcg atgaaggagc tggcggaaga gccgtcgttg cgcgcgaccg
     6301 gcacgatcgt cgaagtcgat catccgacgc ggggcaagta catcacggtc ggtaacccgg
     6361 tcaagctgtc ggcatcacct tccgacgtca agcgatcacc gctcctgggc gagcacaccg
     6421 acgagattct gcgaactgtt gtcgggctcg gcgaggacga catcgccgcc gcccgcgagg
     6481 aaggcgccat ctaggcgttc tcagcgtcgc gccactccat ctcacaagcg taggtcgaca
     6541 cgccactctt gccctcgggc aggggtggcg tgtccatttc tgagcgactg aaccatttat
     6601 gctacgcatg tacgtacgcc tgcatcggtc tcgtgcttcg cgcggtgaga agaacccgtt
     6661 ccaatcctgc cttggaaagg gagcggcttt gcgccgcacg gaatttgaag cccctctcct
     6721 cgcaggagag gggttgtcga ggggttttcg acacggcgcc aagcacctcc gttacctacc
     6781 aaattccagt caagcacatc caccgatttt cgcgtacgaa tgcgaaaaat ggtataatca
     6841 agtgtctgcg gaccggaggg aagtgccttt gctgcggctg tcggaagacg agtttgaaga
     6901 gctggttgtc gcggcgctgg acacccttcc cgaagacctt ctggagttga tggacaacat
     6961 cgaggtcacg gtcgaacggg cgccaagcag acagcagatg cggcgagtgg gactgcgatc
     7021 cgacaccctg ctcggtttct atgaagggat cccgctgacg gcgcgcaaca gctcatcgta
     7081 ttcgctggtc gtcccggaca agatcacgat cttccagcgc cccatcgagg gaatctgctc
     7141 gacgcatgac gaggtcgtgg cccaggtccg caagaccgtg atccatgagg tggcccacca
     7201 tttcgggatc ggcgaagagc gactgcgtga actcggctgg ggctgacaag gtcggcaaca
     7261 atgcacgact ggggcagaga agacgaggcg ggctcgaccc gcgatggccg catcgaccgc
     7321 gatgcgccgc tgccgggcgt gatcgacacg atcggcttcg gatacgccgc gctggcgctg
     7381 cgcccgctgg cgattctgcc actcgttgcg ctggaactgt acctgctcct cggaccgaag
     7441 gttgcgctgg ctccgttgac caacggcctg gcggacctgc ttcgccggcg agggagccat
     7501 tgggacgatc tggcgggtga aattggacgg ttggaaggat tcaacatctt tgaggtcgga
     7561 tcgctggaaa ttccgctatt gcgaacgccg ggcatcgtcc cgaccttctc cggcgacgaa
     7621 attgccaatc tccggatcgg cgccaactgg tcgagcctgc cgtggggcgt cgttgtgctg
     7681 ctgctgatcg catcactcat cgtcgggttg ctgctggcag tcgcctaccg ctcgctgctg
     7741 gctcaggcgg cactggcgac gacgaatcag tcgatcgcga tcggtccgaa ggcgatcgcg
     7801 cgccgagcgt ggcggatcgc tggctgggga ttggcgctgg ccgggctggc gctgctggtg
     7861 gcaatgccgg tcctggtcgc caccatcgcg ggcgcggtca ttgggctcgg cgaattgacg
     7921 ttgctgtggc tgttcctgct ctttccggcg acctgggcct gggtccactt ctacttctcg
     7981 gtccatgccc tggtcatcac cgacgccgga ccgctggagt cgctgcgtgc cagctatcag
     8041 gtcgtccagc atcatttctg gcagtcgctg cgcttcatgg ctgcctcact cctgattacg
     8101 accggattgt cgttcgcgct gcgggagctg gcgacaagct tgccgggagc gctgctggca
     8161 atcctgctca acgccgttat cgcgagcggc atgatcgtcg ccgctatgtt gttctaccgc
     8221 gaccgggcag atcgtctcgg cctacacgtt caagtcccag gaaggtaacg tacacgtggc
     8281 acaggcagtg aaaacaaatc caaccgcacc ggccaggaag agttacgacg caaaggacat
     8341 cgaggttctt gagggcctcg aagccgtgcg tcgccggccg ggcatgtata tcggcagcac
     8401 cgacctgcgt ggcctgcatc acctgatcac cgaggtcgtt gacaacgcca tcgacgaggc
     8461 gatcggcggc tactgcgaca cggttcggat cacgatccac cccgacgcgt cggtgacggt
     8521 ccacgacaac gggcgcggca tcccgaccgg caagcatccg aagctcaaca cgtcggcgct
     8581 ggaagtcgtg atgacgatcc tgcacgcagg cggtaagttc ggcggcgacg gctacaagat
     8641 gtcgtccggc ctgcacggcg tcggtgtgtc ggtcgtcaat ggcctgtcgg actggatggt
     8701 ctccgaggtc cgccgcgatg gcaagctgca tcgccaggag taccggcgcg gtgtcccgac
     8761 cgggccggtc cgcaaggtcg ggtcggtcca tccggacgac cacggcacga cccagacctg
     8821 gatgccggat gcgaccatct tcgacacgct cgactacaac ttcgaggctc tggcgcagcg
     8881 cttccgcgag atggcctatc tgacaaaggg gttgcggatc gtcttcaccg acgagcgcag
     8941 cgaccgcgag atgacgttct acttcgaggg cggcatccag tcgtatgtcc ggcatctcaa
     9001 taagacccgc aacgtgctgc atccgaagcc gttctacatg caccgccagg tcggcgactt
     9061 catcgttgag gtggcgttgc agtacaacga cacctacggc gagtcgacgc atacgtttgc
     9121 caacaacatc aacacggtcg acggcggcac gcacctgacc ggcttcaagt cggcgctgac
     9181 gcgaaccatc aacgactacg cccgcaagaa cggctttctg aaggagaacg acgacagcct
     9241 gagtggcgag gacgttcgtg aagggttgac ggcgatcgtc agcgtcaagt tgccggagcc
     9301 gcagttcgag gggcagacga agggcaagct cggcaacgca ccgatggcgg gcgccgtcca
     9361 gtcggtcgtc aacgagctgc tggcgcagca ccttgaggag aacccgcagg ccgccaagcg
     9421 gatcgtcgac aagtgcatca cggccgcccg cgcccgcgac gcggcccgca aggcgcgcga
     9481 tctggtccag cgcaagggca gcctcgacac gttctcgctg cccggcaagc ttgccgactg
     9541 ctcggagcgc gatccggccc gtgccgagct gtacatcgtc gagggtgact cggccggtgg
     9601 ctcggccaag caggggcgtg accgccgctt ccaggcgatc ctgccgctac ggggcaagat
     9661 cctcaacgtt gagaaggcac gcctcgacaa gatgttgcag aatgcggaga tccgggcgct
     9721 gatcacggcg ctgggaaccg gcatcggcga cgggttcgac gccagcaagc tacgctatca
     9781 ccggatcatc atcatgactg acgccgacgt cgacggcgcc catatccgga cgctgctgct
     9841 gacgttcttc ttccggcaca tggagagcct gatcgtcgac ggccgtctct acatcgccca
     9901 gccgccgctc tatcgcctcc agaagggcaa tgatgtccgc tgggtctaca acgacgaaga
     9961 gcgcgaccgg gtcatgaagg agttcaagga cggcagggtc gagatccagc gctacaaggg
    10021 tctcggcgag atgaaccccg agcagctctg ggagacgacg atggacacgg catcgcggac
    10081 cctcctgcag gtcacgatcg aggacgcggt ccattcggac gagacgttcg acatgctgat
    10141 ggggaacgct gtcccgcccc gcaagcgctt cattcagacg cacgcccgcg aagtccagaa
    10201 cctggacgtg taggcgaggc atcctgacac ccccggttcg ggccgagatg ggccgaaccg
    10261 ggggtgtttc gcgtctggca ggagagtgtt acattttcca ggttccggga cgcactcggt
    10321 gtcgtggatg accttcacgc gttcagagag aatgacagca tgcggatcgc gctcgacaca
    10381 catggcggag actttggggt gccacggaac atctccgctg cactcgacat tgccactggt
    10441 tccgatacga acctggtcct ggttggccac cggaacgaga tcgaagccga actgcggcgc
    10501 tgtaacacac ccgtcaacgc atttgagatc gtccacgccg aagagcagat cgggatgggc
    10561 gaggcggccg ttcaggccgt ccggcgcaag gccgacaact cgatcacggt cgcactgggg
    10621 ctgatgaagt ccggtgccgt cgacgcggtc gtttcggccg gccacagcgg ggcggttgtc
    10681 gcggcggcac tgatgacgct cggccgaatc cgcggcgtct cgcggcctcc actcggtacg
    10741 cggatcccag ccatcaacac ccacaactca ttcgcgctcg acatcggcgc cgtcatcgac
    10801 cccaagccgg agatgctgtt gcagttcggc tacctcggcg ctgcctactc caaggccgtt
    10861 ctagacgtcc cgaatccgag cgtcgcgctg ctctcgaacg gcgaggaatc cagcaagggc
    10921 aacgctctga ccagagcggc gcaggagctg ttcgagaggt ccgatctcaa ttacatcggc
    10981 tacgtcgagg gcggcgagat gctcgacaag ccgccggccg tgaccgtcac cgacggcttc
    11041 accggcaaca tcgggctgaa gatcgccgaa gggacggcag ccagcatcca gcacgctctc
    11101 cggcgtgagc tgaccgccaa ttggcgcgac aagctgctgg caagccttct gcggcctgct
    11161 ttccgccgca tgcgcgacaa tatggacttc gaggggatcg gcggcgcggc gctgctgggc
    11221 gtcaacggtg tcgtgatcgt cgcccacggc cggtcgacga cgcgcggcat cgtcagcgcg
    11281 atccggacgg ccgagcatgc cgccgccgtt cgactccccg aactgctgcg agacgcgatt
    11341 cccgttcgcg accagcggct cgccgagccc agcgacacga cggcgaccgt cagctaacgg
    11401 gacgttgaac ctctcgtcac gacccaatct ccaatccggt acgggcgata ttgcgatatc
    11461 gcccttcgtg tccccgccga ggcgttcggg cagggaacag ggcgatattg cgatatcgcc
    11521 cgtaccggat tggggattgg gtcgttgaag gcgttacgaa tcgatggact gtcaagctcg
    11581 aatcgacgga tcttctgatt gttccgcaat gtgtccgaga tcttgcagcc aggtttcgat
    11641 ctcgtccgtc gagcggaggc agatgacggt caggtgcgcc cactgcgggt cgcgcgacat
    11701 cgcggaatag tcgcgacggc ggcggcgatg ggtgcggatg atgtgggaaa tcagcgacct
    11761 gtcggtgtcc cagagcatca ggtgctcgac gaagcgttca cggttgtcgc cgccgtagag
    11821 ttgttcctgg ctgcgccagc ggctccagga gcgaccgacg cagcgacgaa ggagtgtggc
    11881 gaggggaaga tcgatccaga cgatcgtgtc ggcacggggc cagctgacgt cctgctgttt
    11941 cggcaggtag ttgccgtcca tcacccagcg ttgcgaatcg gtcgcggtcc ggacgcggtc
    12001 ctggaagacc gcgagttccg cttcgcgcca accggattcc cagtgaagcg cgtcaagctc
    12061 gatcatcggt gcgcccagga taccggcgag gcgggtagca agtgtcgtct tgccggcgtt
    12121 ggagctgccg atgacgatga tgcgctgggg aacatggcca ttcacgaaca cctccgcagc
    12181 gagatgcggt gacgacgaga caggctcccc gttcccgagg agcctgtcga gattcaatgc
    12241 tggaatctga gagttatgcg tcgtcgccgc cgacggctcg cttgagttgc gtgccaggcc
    12301 ggaacgacgg gctcttgcga gcggcgatcg tgatgcgctc gccggtctgc ggattgcggc
    12361 cctcgcgggc gccgcgctcg gaaatcctga acgagccaaa gccggtgatc gtcacttcct
    12421 cacctgccgc gagctggtcg cggatcgcgt cgaaggtcgc ctggacgatg ttcgagacct
    12481 gtccctccgg catgttggtg ctcgtggcga cggccttgac cagatcctgc ttcctcatgc
    12541 tgttgtgcct cctcatcccg gtcgaaccgc gctgcctcag ttgcaacggc ggcgggcgta
    12601 gggagcgact atagcacaga tgagcgaaga gccaaaggcg atatccgcgt atgtacgcgg
    12661 aaaatcacga tcgtcgccgc ctttggcggg caacttcgaa cgcaatgatt gatcctgcga
    12721 cggcagcgtt caaggattcg actcccggcg ccatcgggat cgaaacgagt tcatccgcgt
    12781 acgtacgcgc ctcctgaccg gcgccttcgg cctctccacc gatgatgagg agaacgggag
    12841 cggtccagtc gacgtcgtcg tacgctcgct tcccgtcaga atcggcgatg atgacgctgg
    12901 gaagcgatat gaagctgccg gacgtgattt cgaagatgct ggagaacgat tgaaccggca
    12961 gcgcgaagat cgaaccaacc gacgcacgga cggccttcgg gttgaacgga tccgcgctcc
    13021 caggcagcag tgcgacgccg tcagcgccgg cggcagcact gctacggacc agcgtaccga
    13081 ggttgccggg atcgcggacg ccgtcgaggg cgacgacaaa gctacagtcg actggaagga
    13141 ctgactcagg catcgcgcag atggcggcga gcggttgcgg atgctcggtg tcggtgatct
    13201 gcttgaagag tggggcatcg atcagcagcg ttcgtgacgc caatgcgctc agtgtcgcga
    13261 cgagggccgg gtcgagttca tcctggcgcg aggcgtcgat catgaatgcg cggatctgga
    13321 cgccgaggtc agaggcggtc cggatggagc gaaccccttc aacaagcaac gcccgctcgc
    13381 gataacgcac ccgtttccgg tgcagcgagc gggccagctt gatggtcggg ttggctggac
    13441 tggtgatcag ttcggcctga cgatggacgc ggtcgtcacc catcccggcg ctctcagctc
    13501 gtgccgcgtg cgacctcgac gaactttgag aatgcagcgg cgtcgcgaac ggcgaggtcg
    13561 gccaggatct tgcgatcgac ggtgacaccg gccttgtgca gcccctcgat gaagcggccg
    13621 taggtcatgc cgttggcacg ggcagcggcg ttgatgcgcg tgatccagag acgacgcatc
    13681 tggcgcttgc gcgtccggcg gtcgcgatac tggtacatca acgagcgcat gactgcttcg
    13741 ttggcgcgct tgaagaggcg gttgcgtgtc ccacggaacc cctcggcgcg attgagcacc
    13801 gcgcggtgcc tgcgccgggt ggtcacgcca cgcttgattc gagccatctg tcaaatcctt
    13861 ccgaatcgtc accgggccag tcaggcggcc ggaacgtact ggtctactcg gtgccgtacg
    13921 gcaacagtcg cttgatgcgc ttcttgtcgg cggcgttgac ctcgaacatc ttgtcgaaca
    13981 tccgccgggt gcgcggcgac ttcctgcggc ggaagtggct cttgccgccc tttgcacgca
    14041 tgaccttgcc ggtaccggtg atcttgaacc ggcgtgcggc ctgcttgttg gttttcatct
    14101 tcggcatgct accgccccct cgactccctg tatcccgacc aatgtttcat gacaccggaa
    14161 cggcgccgca gcgcggcgcc gcccgtcgcg gatcgtcgaa aaccagtcta ccctttggtc
    14221 tgagcctccg gctggggtgg ttcgccctga gccagcctgg ccttctcgac gtcggccggg
    14281 ctgacgccgt cgaccttgac aaactctttc ttcggactca gcaggaccga catcgttctg
    14341 ccttccatcc gagcatcctg gtccaccgta ctgacaggct gaagctgagc aacgacctgt
    14401 ttgagcaact cgcgaccgat ctcctggtgc agcatctcgc ggccccggaa cagaacagcg
    14461 accttgacct tgtcgccgtc ttcgaggaag cgctgcgcct gacgggcctt cgtgtgcaga
    14521 tcgtgatcgc cgatcttcgg atagatccgc acttccttga ggaccgcgcc cttggacttc
    14581 ttgcgcgact cccgttcctt gcggctctcc tcataccgga accgcccata atccatgagc
    14641 cggcagacgg gcgggacggc gttgggctgc acctcgacga ggtcgaggcc acgttcccgg
    14701 gcgatttcca gggcatcacg agtctggaca accccgagct gcgttccgtc ctcatcgatc
    14761 agacgaacct cgcggacacg aatgcgctcg ttgactcgaa ctggtctggt gcttatggcg
    14821 tacctcctgt aggacgatca tgttctggcg ctgatttacg gacaacacca agcttccccg
    14881 cgatggggac aggctgaaat ggtaccagaa tctgccggtt cggttcaatc ctcgccgacc
    14941 agaatcgggt cgccgggatg cgcgaaaatc gtcgtctgct cgctgacgaa agcgatttcc
    15001 cagcccgctg cgcgcagcgt cgcgactgga tcgccggtcc gctgtcgggt caggttacag
    15061 tctgttgtgc cttggatctc ctgacggccg aggatgacga agcgggcgtc ggtctcgtcg
    15121 gcagtgacgg ttccgtcgat gatcgcgttc gcctgcgcgg cgcgcaggtc gagaatgtcg
    15181 cggatggcgg cgtactcgcc ccgccgccac tgcgactcgt ggccgtgcca gccgaggatg
    15241 gttgggacac cgctgaatgc tgagatacgg ctcagcggcg cgccagcggc gttgatgtac
    15301 gagcagcccg gctgctcgac gatcgtgtcg cccggctcgg atgattccgc gatccagtcg
    15361 atcgccgcca gatcgcccgg cgcactacgg ctgagatacg cgctgccatt gaggccgtag
    15421 cgcgtggcga agtcgttcgt ccagtcctga gctgagagcg gtgtgtaggg cagcgtcagc
    15481 aggacgagca acccgagcgc ggcaaaggcc ggtacccgat aagcgcgggg aatttgcgtc
    15541 gcgacgtaga cgatcgtcgc ggcgctggcg atgctgagca gcagccaggc ctgataaaag
    15601 agcttgaaga cggtgttcat ccggtcattg aagacatcct ggatgtagac gaactccggg
    15661 atgagcgcca gcacgaaacc ggccgcgaag agaccggcaa ggacgcgagc cggttggcga
    15721 tccgaatggg cggcgaacca gagggcgagc gccagcggca cacccagcag caggacagcc
    15781 ggcgcccaag ccatcgcaac gccgaaggca aggatggcga ccgccagcca gatctgcggg
    15841 cggcgacgga agatcatgag taacgcccgg tcccgtacaa gctcgatcgc ggccaatccg
    15901 gcaaacgccg caagccaggc gccgtggacg atcaggagct cgcggacgct cgacggccgc
    15961 caactgacga tggcgaaggt gtcgaaggcg cgaccgacga ctggcaaatc ggccagccag
    16021 gccggcacgc tcgcgttcgg aacgccgacc ggcgcgctga actcgagcat gaatggtccg
    16081 gccatgagca gcgcaccgcc agcgacgatc agcagatcgc gcacgatcgg ccgccacaga
    16141 tagcgatgct tgagcgccag cccgccagcg accagcagca tgccagccgg ggcgtcccaa
    16201 ctgttcgacg cgtagagcag gccaaccagc gcgccgaacg cggccagtcg cgtccacggt
    16261 tccggctcgc ggaacaaggc aagcgagagt gccagaacga gaacggcggc cagcagcgga
    16321 taggtcagga catgcgggtg caggtcgcca agcacatacg agaaggccgg gaactcgttg
    16381 atggtctgtt tctcgtcgcc cggctcgttg acgtcgttgt catagatgat ccggcttgcc
    16441 tgccagccga cgccatccca ccagccggcg tcgatcgtgg cgccggggtc gtcgatcatc
    16501 cgtcgggccg tctcgaggtt ggcgcccagc agcagcagca cgactgacag ggcggctccg
    16561 acgatcacca gacgccggcc gctgcccagc gcccgcagca gcgtcacgcc ggcagcggcc
    16621 gctaccgtcg cgccggaggc gaacagcgtc gccagtgcca gattgaagcc agtcgcggtc
    16681 gggatggtcg agagcttgac gat
//
LOCUS       DASUGJ010000458         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1183098, whole genome shotgun sequence.
ACCESSION   DASUGJ010000458 DASUGJ010000000
VERSION     DASUGJ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183098"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFV93_10080"
     CDS             <1..262
                     /locus_tag="VFV93_10080"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX5165733.1"
                     /translation="GMPEPAARAETVKNSMFPGAAVMYLLGTDMIRDLRAELERRQGS
                     DFTLARFHDSFLAHGSIPVALTAKLMRGDPIVADGYASPQLP"
     gene            complement(295..603)
                     /locus_tag="VFV93_10085"
     CDS             complement(295..603)
                     /locus_tag="VFV93_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165734.1"
                     /translation="MAVIRQVDAQMIGRLYAEMVEDDPDVQEIYVRPFGDSIEVWMLT
                     SHSNYDAEDAIFDAAVAVQRRVPDTRIDLHVVNPRHYPTTCDLVRDIIPDNAVAVPAT
                     "
     gene            complement(709..1173)
                     /locus_tag="VFV93_10090"
     CDS             complement(709..1173)
                     /locus_tag="VFV93_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX5165735.1"
                     /translation="MTNDDAVRELYAQLLTSWNQRDAQAYAALLAGDGLVIGFDGSTM
                     HGPAETEQQLGQIFGHHQTAPYYWKVRRIRELAPGVAVLHAVVGMVPPGQSDINPATN
                     AIQTMTAVQRDGQWRIALFQNTPAAFHGRPEASEALTQELREVYRAHAATTG"
     gene            complement(1212..1691)
                     /locus_tag="VFV93_10095"
     CDS             complement(1212..1691)
                     /locus_tag="VFV93_10095"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX5165736.1"
                     /translation="MQHDQTEGPDVITIPPLIPLAGLGLSLVGRMLVNMPILPRWLAG
                     PRKRAGIALIVGGFLTGLWGARTFREAGTHINPEHAATTLVESGPFQYTRNPIYIGMS
                     AIYSGITLLLNNMWGVLLLPGVIAALQRGVIEREEAHMRERFGDAYEEYTERVPRWL"
     gene            complement(1710..>2138)
                     /locus_tag="VFV93_10100"
     CDS             complement(1710..>2138)
                     /locus_tag="VFV93_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642266.1"
                     /note="catalyzed the formation of 2-ketoglutarate from
                     2-hydroxyglutarate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX5165737.1"
                     /translation="HFTPRMNGDVWLGPNAVLAFSREGYRFRDIRPKDTGELFTNGGF
                     RRFARQHWRTGLSEMARDLSRSRFLASLQKFIPELTQDDLLPGPAGIRAQALSPDGKL
                     VDDFVIDRGEGVLHVRNAPSPAATSSLQIGAMIADAVEEM"
BASE COUNT          351 a          696 c          704 g          387 t
ORIGIN      
        1 cggcatgccg gaacctgccg cgcgggccga gacggtcaag aactcgatgt tccctggcgc
       61 ggcggtcatg tacctgctcg gcaccgacat gatccgtgac ctgcgcgcgg aactggagcg
      121 ccgccagggc agcgacttca ccctcgctcg cttccacgac tcgttcctcg cccacggctc
      181 gatcccggtt gcattgaccg ccaagctgat gcgcggcgac ccgatcgtcg ccgacggcta
      241 cgccagcccg cagctgccgt gattcagcga ctcgtgcgtg gtctcagatt gttatcaggt
      301 cgctggaacg gcgacggcgt tgtcggggat gatgtctctc acgaggtcgc atgtggttgg
      361 gtagtgacgt ggattgacaa cgtgaagatc gatacgcgta tccggtacac ggcgctggac
      421 tgcgacggca gcgtcgaaga tggcatcctc ggcatcatag ttggaatgtg atgtcagcat
      481 ccacacctcg atggaatcac cgaaagggcg aacgtaaatc tcctgcacat cagggtcatc
      541 ctcaaccatc tcggcgtaga ggcgtccgat catctgtgcg tcaacctgtc gtatgactgc
      601 catcgctcgt gttcccatcg tttgccgcac gtacgcagtg ggcgtgatgt aatgcgccca
      661 caagtataga cggctcaagc agcacagcca gcacgggcga ggagccgatt atcccgtggt
      721 cgccgcgtgc gcccggtaga cctcgcgtag ctcctgggtc agggcctcgc tggcctctgg
      781 gcgaccgtgg aaggcggctg gcgtgttctg gaagagcgcg atccgccatt ggccgtcgcg
      841 ctggacggcc gtcattgtct ggatggcgtt ggtggccggg ttgatgtctg actgccccgg
      901 cgggaccatt ccgacgacgg cgtgcaggac ggcgacgccc ggcgccagtt cgcgaatcct
      961 gcgtaccttc cagtagtagg gcgccgtttg atggtggccg aagatctggc ctagctgctg
     1021 ctcggtctcg gctggtccgt gcatcgtgct gccgtcgaag ccgatcacca gcccgtcgcc
     1081 tgcgaggagc gccgcatacg cctgagcatc tcgctggttc cagctcgtca gcagctgcgc
     1141 gtagagttcg cggaccgcgt cgtcgttcgt catacgttgc gacacttcca tctgctcgtc
     1201 cgcgccgatc gctacagcca gcggggaacc cgctcggtgt actcctcgta ggcgtcgccg
     1261 aagcgttctc gcatgtgcgc ttcctcgcgc tcgatgacgc cgcgctggag ggcggcgatg
     1321 acgcccggca gcagcaggac gccccacatg ttgttgagca gcagcgtgat accgctgtag
     1381 atcgcgctca tgccgatgta gatcgggttg cgcgtgtact ggaatggacc actctcgacc
     1441 agcgtcgtcg cggcatgctc cgggttgatg tgggtgccgg cctcccggaa ggttcgcgcg
     1501 ccccagagcc cggtcagaaa gccgcccacg atcagcgcga tcccggcccg cttgcgcggc
     1561 cccgccagcc agcgcggcag gatcggcatg ttcacgagca tccgcccgac gagcgacagc
     1621 cccagccccg ccagcgggat caacggcggg atcgtgatga catccggtcc ttcggtctgg
     1681 tcatgctgca tcaggttccc tcctccgggc tacatctcct caaccgcgtc agcgatcatc
     1741 gcgccgatct gcagcgacga ggtcgcggcc ggcgacgggg cgttgcgcac atgcaggacg
     1801 ccctcgccac ggtcgatcac gaagtcgtcg accagcttgc catctggtga caacgcctgg
     1861 gcgcggatgc cggccgggcc gggcagcagg tcatcctgcg tcagttccgg gatgaacttc
     1921 tggagcgagg ccaggaagcg cgagcgcgac aggtcacgcg ccatctcgga caggccagtc
     1981 cgccagtgct ggcgggcgaa gcgacggaag ccgccgttgg tgaacagctc gccggtatcc
     2041 ttcggccgga tatcgcggaa gcggtacccc tcgcgcgaga aggccaggac ggcattcggg
     2101 ccaagccaga catcgccgtt catgcgtggc gtgaagtg
//
LOCUS       DASUGJ010000459         3876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_905881, whole genome shotgun sequence.
ACCESSION   DASUGJ010000459 DASUGJ010000000
VERSION     DASUGJ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905881"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFV93_10105"
     CDS             complement(<1..324)
                     /locus_tag="VFV93_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165738.1"
                     /translation="MVTRVAVVGSCASGKSSVVARLREQGVEAWAVAQEHSAIAALWR
                     HQQPDILVFLDTTLATVRARRDDDNWPAWIFTVQQERLANARANADLIVPTDDLTLDE
                     VVETIL"
     gene            complement(324..1043)
                     /locus_tag="VFV93_10110"
     CDS             complement(324..1043)
                     /locus_tag="VFV93_10110"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX5165739.1"
                     /translation="MTQILSIEIAVAKTHKYASRDSGDTAETVERPTGGVSMVLVDGQ
                     GSGQAAKSLSMMVSSKAVSLLKEGVRDGAIARATHDFLHAYRHGRVSATLDIVSVDLA
                     AKTILVTRNSATPYLVRRNGDVERQESTSGPIGLYRHTRPAIDQYAIEAGLTVVIFSD
                     GIVNAGHRSGRPFDALAFVRDSLPPDALAHQLADGVLEAAIGVDQGRPNDDMTVAAVT
                     IVPMADEQPVRTIRVSVPFTS"
     gene            complement(1040..2065)
                     /gene="rfbB"
                     /locus_tag="VFV93_10115"
     CDS             complement(1040..2065)
                     /gene="rfbB"
                     /locus_tag="VFV93_10115"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX5165740.1"
                     /translation="MTGERIRSVVVTGGAGFIGSTFARLLIERGYEHVRVFDKLTYAG
                     NPANIDSLLSHPAFSFVQGDICDAGAVDDAIAGFDAIVNFAAETHVDRSLMDPGEFIQ
                     TDVYGVFTLLEAARRQNPRRFVHVSTDEVYGEISRGSSTESDPLAPRNPYSASKAGGE
                     MMVRAYVSTYGVPAIISRGSNTYGPYQYPEKLIPLAVTNVLQGVPIPIYGDGRQIRDW
                     IHARDHAGGIEFLLRHGEPGEAYNIGGGNERENLSVAQIILSELDAPAEMLTHVTDRQ
                     GHDRRYSLDTSRLRGLGWRPEVDFEQGLRETIQWYRDNRSWWEPIKSGEFESYYQSNY
                     AAREKIH"
     gene            2186..2917
                     /locus_tag="VFV93_10120"
     CDS             2186..2917
                     /locus_tag="VFV93_10120"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5165741.1"
                     /translation="MPLKAVTFDFHNTIANCDEWFQLEVRELVPSVLDWLAEHDEAIV
                     SDGDRDEAIRHYRALRLDIMEHGNERDAYDCTLDVLARMGRSVDEQTVRLGVDAVMRT
                     ALPGSTPVEGVVAAVRDLRAREIELAVVSSAVHHAFLEWSLAKFGILDCFSHVITSAS
                     CGFYKSRTEIYQHALDQLAVAPSEAVHVGDSHRYDVETATRAGMRTVWFSRGQADSPS
                     NGAGLTVTSLEGLAPLILDHFGSGL"
     gene            2917..3528
                     /locus_tag="VFV93_10125"
     CDS             2917..3528
                     /locus_tag="VFV93_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165742.1"
                     /translation="MRFDIFTIFPGIFSGPFDESIVRRAIDRGLLEIALHDIREHGLG
                     RHRAVDDTPYGGGAGMVMAAPPLFASIETALGDALAETPVMLMSPSGEPFTQQIAREL
                     SELPRLALVCGRYEGVDQRVRDHLITRELSIGDYVLTGGELAAAVIVEVVPVPTMTGV
                     RPAICCIVSAACCSRSLRFMCMNSPVVARMNNPWTPDSIRKSW"
     gene            complement(3661..>3876)
                     /locus_tag="VFV93_10130"
     CDS             complement(3661..>3876)
                     /locus_tag="VFV93_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5165743.1"
                     /translation="IEAESLEEESFSEGLLEYPHYTRPASFRGWEVPPVLVSGHHGEI
                     ARWRREQALELTRQRRPDLLAGPDQPH"
BASE COUNT          653 a         1212 c         1298 g          713 t
ORIGIN      
        1 caggatcgtc tcgacgacct cgtccaacgt caggtcgtcg gtcgggacga tcaggtcggc
       61 gttggcgcgg gcgttcgcca gccgctcctg ctggaccgtg aagatccagg ccggccagtt
      121 gtcgtcgtca cgacgggcgc ggacggtcgc gagcgttgtg tcgaggaaga cgaggatgtc
      181 cggctgctga tggcgccaga gggccgcaat cgccgagtgt tcctgggcga ccgcccaggc
      241 ctcgaccccc tgctcgcgca ggcgggcgac gaccgacgac ttcccagacg cgcaactgcc
      301 gacgacggcg acgcgggtga ccatcagctt gtaaacggga cgctgacgcg gatcgtccgg
      361 acgggctgct catcagccat tggaacaatc gtgacggccg caaccgtcat gtcgtcgttt
      421 ggcctcccct gatcgacccc aatcgcggcc tccagcacgc cgtctgccag ctggtgagcc
      481 agcgcatcgg gtggtagtga atctcgtacg aaggccaacg catcgaacgg ccggccggag
      541 cggtgaccgg cattgacgat gccgtccgag aagatcacga cggtcaagcc ggcctcgatc
      601 gcgtactgat cgatggcagg ccgcgtgtgg cggtagaggc cgatcgggcc ggatgtactc
      661 tcctggcgct cgacatcgcc gttgcggcgc accaggtacg gtgtcgcgct gttgcgggtg
      721 acgaggatcg tcttggcggc cagatcgacc gagacgatgt cgagcgtcgc cgaaacgcgg
      781 ccgtgccggt aagcgtgcag gaaatcgtgt gttgcccggg cgatcgcgcc atcgcgaacg
      841 ccctccttga ggagcgagac ggccttgctt gagaccatca tgctgagtga cttggcggcc
      901 tgtccggagc cctgaccgtc gaccagcacc atcgagacac cgccggtcgg gcgctcgacc
      961 gtctcggcag tgtcgccgct gtcgcgtgat gcgtacttgt gggtcttggc gacggcgatt
     1021 tcgatggaga gaatctgcgt caatgaatct tctcacgcgc ggcgtagttc gattgatagt
     1081 acgactcaaa ctcaccggat ttaatcggtt cccaccagct gcggttgtct cgataccact
     1141 ggatcgtctc gcgtagcccc tgctcgaagt cgacctctgg ccgccagccg agcccgcgca
     1201 ggcgcgacgt gtcgagtgag tagcgccggt catgcccctg ccggtcggtg acgtgggtca
     1261 gcatctcggc cggcgcgtcc agctcgctga ggatgatctg agcgacggag agattctccc
     1321 gttcgttgcc cccaccgatg ttgtacgcct cgcctggctc gccatgtcgc aacaggaact
     1381 cgatcccgcc ggcatggtcg cgagcgtgga tccagtcgcg gatctggcgg ccgtcgccgt
     1441 aaatcgggat cgggacgccc tgcagcacgt tcgtgacggc cagcgggatc agcttctctg
     1501 ggtactggta cggcccatac gtgttcgagc cgcgcgagat gatggctggg acgccgtagg
     1561 tcgagacgta ggcgcggacc atcatctccc cgccggcctt gctggccgag taggggttgc
     1621 gcggcgcgag cgggtcagac tcggtcgaag agcccctgct gatctcgccg tagacttcat
     1681 cggtgctgac gtggacgaag cgtcgcggat tttgtcgacg ggccgcctcc agcagcgtga
     1741 aaacgccgta gacatcggtc tggatgaact cgccagggtc catcaacgag cgatcgacgt
     1801 gcgtctcggc cgcgaagttg acgatcgcgt cgaagcccgc gatggcatca tcaacggcgc
     1861 cggcgtcaca gatgtcgccc tggacgaacg agaacgccgg atggctgagg aggctgtcga
     1921 tgttcgcggg attgcccgcg tatgtgagct tatcgaacac ccgcacgtgc tcgtagccgc
     1981 gctcgatcag gagccgcgcg aaggtactgc cgatgaatcc cgcgccgccg gttacgacga
     2041 cgctccggat acgttcccct gtcatgtcac tcccgccttc aggcgcgaac attgcaagca
     2101 gcctgctcca gccgggccga accaggcttc gacgctatca tattcttcat agtgtggcag
     2161 atcatcacgt gagaaaggga tgacaatgcc gctcaaagcg gtgacatttg attttcataa
     2221 cacaattgcg aactgtgacg agtggtttca gctggaggtt cgagagctgg tgccaagcgt
     2281 gctcgactgg cttgccgaac acgacgaggc gatcgtcagc gacggggacc gcgatgaggc
     2341 catccgccac taccgtgcgc tgcggctcga catcatggag catgggaacg agcgcgatgc
     2401 ctacgactgc acgctggacg ttctcgcccg gatgggccgg agcgttgacg agcagacggt
     2461 ccggttgggc gtcgacgcgg ttatgcggac ggcgttgccc ggatcgacac ccgttgaggg
     2521 cgtcgtcgcg gctgttcgcg acctgcgcgc ccgcgagatc gagctggcgg tcgtgtcgag
     2581 tgccgtccac cacgcgtttc tcgaatggtc gctggcgaag ttcggaattc tcgattgctt
     2641 ctcgcacgtc atcacgtcgg ccagttgcgg gttctacaag tcacggaccg agatctacca
     2701 gcatgcgctg gaccagctcg ccgtcgcgcc atcggaagcc gtgcatgtcg gcgactcgca
     2761 ccgctacgac gtcgagacgg caaccagggc cgggatgcgg acggtctggt tctcacgcgg
     2821 gcaggcggat tcgccatcaa acggcgcggg actgacagtt accagtcttg aagggctggc
     2881 gccgctgatc ctggaccact tcgggagtgg tttgtaatgc gcttcgacat cttcacgatc
     2941 ttcccgggca tcttcagcgg accgttcgac gagtcgatcg tccgtcgcgc gatcgatcgc
     3001 gggctgctgg agatcgcact gcatgatatt cgcgagcatg gtctgggccg ccaccgggcc
     3061 gtcgatgaca cgccctacgg cggtggcgcc gggatggtga tggcggcgcc gccgttgttc
     3121 gcctcgatcg agacggcgct cggcgacgcg ctggcggaga cgccggtcat gctgatgtcg
     3181 ccgtccggtg agccattcac gcagcagatc gcccgcgagc tgagcgagct gccacggctg
     3241 gcgctggtct gcgggcgcta cgagggcgtc gatcagcgtg tgcgcgatca tctgattacg
     3301 cgtgagctct cgatcggcga ctacgtcctg accggcggcg agctggcggc tgccgtcatc
     3361 gtcgaggtcg tgccggtgcc gacgatgacc ggcgtccgcc cagcgatatg ctgcatcgtc
     3421 tcggccgcgt gctgttcgcg gtctttgaga ttcatgtgca tgaactcgcc ggtcgtcgcc
     3481 aggatgaaca acccctggac gccggactcg atcaggaagt catggtgacg ctggttcgct
     3541 tcgggatcga gcgaaccgtc ctccttgaac agcgtgaccg ttggcggaaa tatgcctcga
     3601 aacatcgcac cccctggcgt ctacaacgag accgatggag tgtagcacgc ggtccgaatc
     3661 tcaatgtggt tggtcaggcc cggctaacag atccggccgc cgctgccgcg tcagctccaa
     3721 cgcctgctcg cgccgccagc gggcgatctc gccgtgatgg ccgctgacga ggactggcgg
     3781 aacctcccag ccgcgaaagc tggcgggccg ggtgtagtgc gggtattcga gcaggccctc
     3841 cgagaaggat tcctcctcca gcgactcggc ctcgat
//
LOCUS       DASUGJ010000460        13991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_905914, whole genome shotgun sequence.
ACCESSION   DASUGJ010000460 DASUGJ010000000
VERSION     DASUGJ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13991
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905914"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFV93_10135"
     CDS             complement(<1..200)
                     /locus_tag="VFV93_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5165744.1"
                     /translation="MIEPILNSDMSGVRRAKHALFFTTRLFVLPALRVGIRLKVFGLE
                     HVPKRGGALIICNHLGWFDPILL"
     gene            complement(197..898)
                     /gene="cmk"
                     /locus_tag="VFV93_10140"
     CDS             complement(197..898)
                     /gene="cmk"
                     /locus_tag="VFV93_10140"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX5165745.1"
                     /translation="MSDRRMTIAIDGPAAAGKSTVGEIVAQRIDGIYFDTGVLYRALT
                     LLALEHGVKPDDAASLATLAATMDICIDRPSVDDGRQVDVLLAGRDLSNSLRTSAIDQ
                     NVSAVSAHALVRAALLEHQRRIARSGRVVMVGRDIGTVVLPDADLKIFLDASPEERAR
                     RRCMQLVEMGKPQPYEDVLADMKRRDRIDSERDVAPLRPASDAIVVDSDPLTIEQVAD
                     LIVEHAEARMQAVCE"
     gene            complement(810..2222)
                     /locus_tag="VFV93_10145"
     CDS             complement(810..2222)
                     /locus_tag="VFV93_10145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5165746.1"
                     /translation="MERLQRVLASAGIASRRRAEELILEGRVRVDGEIVTELGTKVEP
                     GAQKIEVDGKLIPTVRRRYILLNKPSGYITTTSDERSRYTVMDLVPVAERVVPVGRLD
                     RQTEGLLLLTNDGEVANRVMHPRYEIDKEYEALLDGYPPPEVLDRLRRGITIDGQRTS
                     PEMIRPIRNTEDGTIVRIVIHEGRNRIVRKMFDEVGYPVLKLIRTRVGPLQLGAIPRG
                     TFRDLTDGELKVLREALHLTDEDIEFAERVADRPRRDRPDPNRMRTGRPRESNAERAA
                     RGPGGPGRDRRDDRRGGPPRRRDGEQPNRPARPRRDDQYAGPPQARRSDDRRGSSRPT
                     GDDAGERRPARRDDDRRGPARGRDERGADQRGQRGGPRRRDDERRPARSGSGDRRDRP
                     PGPPRGGSRPGGPNRRPTGAPRGPRPGPGPGGQGKHPRHEHPADGAGGGGERPPDDNR
                     DRRTGGGGQEHGRRDRRPED"
     gene            complement(2241..3380)
                     /locus_tag="VFV93_10150"
     CDS             complement(2241..3380)
                     /locus_tag="VFV93_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5165747.1"
                     /translation="MRIAVVLQIAVLLAVGVSGWAPAVAGGRDLAATGSLVRQVAPPS
                     VSAASVLVVDLTTGVEIYSSNADAPLPPASTLKIVTAQVASSILGLDEQLTIQESDIL
                     DPTVFSNMQLQAGDVVDVRTLLHGLLIPSGADAAMALAREAGNRLEPGTADPVDRFVA
                     EMNSWAAAHKMMNSHFTNPVGTDDPQNVASARDLVRATDALLKNWLLARIVAMDSYVA
                     DIGGPNARQVELVSSNQFITRDDVFGIKTGTEENAGQCLITGFWRGDNQLISVVLGSA
                     DRYADTQAVMDAVDGTYRWLALGIGATSQGASDALAAQGLTFLNRRTVLMTVGQAEQV
                     TWEIVIDSEPRGERRGAVVFKLGERVVARMSIYSVGSGAIGPDAA"
     gene            3546..4634
                     /gene="hisC"
                     /locus_tag="VFV93_10155"
     CDS             3546..4634
                     /gene="hisC"
                     /locus_tag="VFV93_10155"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5165748.1"
                     /translation="MLRRIVRESVEQLSTYHPVEAPEELAARLGMPIDAILKLDSNEN
                     PYGCSFRVQEALATFDRYHHYPDAQARSTRERIAAYAGTSVERLIIGNGADELIDLIF
                     LATLDPGDEVVIPVPTFGVYRARAELFGGAAVEVPRLPDFDLDMDALLAAITDRTKLI
                     IITSPNNPTGNLATNQQIVRLLQTGVLVVLDEAYFEMSGKTSLPLVGEFDNLAILRTF
                     SKWAGLAGMRVGYGIFPHFLAEQIWKVKPPFNVSQAGLVAVEASLDDSEYLHGTLNRI
                     RAERSRLFRKLRRIEYLNPYPSQGNYFLCRVTRGDAHDIHLRLADRGIMVRAYGDPLL
                     RDCLRITVGKPEDTDRLIAALQTIGAHV"
     gene            4627..5229
                     /gene="hisB"
                     /locus_tag="VFV93_10160"
     CDS             4627..5229
                     /gene="hisB"
                     /locus_tag="VFV93_10160"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX5165749.1"
                     /translation="MSERRATIARATAETRIHLSFSIDGSGTSRINTGVGALDHFLTL
                     FARHGLFDLDLDVDGDLAVDTHHTVEDTAICLGQAVREAVGDHRGIRRTANVAVPMDE
                     ALAHVAVDCGGRSSYVQNGSFSAPAVGQLETDLVRHFFDSLAREARINIHVITLYGSN
                     AHHEVEAVFKAFARALDAATTLDHRLGDRLPSTKEHIERS"
     gene            complement(5248..5973)
                     /gene="rph"
                     /locus_tag="VFV93_10165"
     CDS             complement(5248..5973)
                     /gene="rph"
                     /locus_tag="VFV93_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5165750.1"
                     /translation="MSITKQRVGREAHEMRPVTIETGVAPYAEGSALIAFGTTRVWCT
                     ASIEERVPPWLVGKRSGWVTAEYAMLPRATHTRTNRDRATNSGRSQEISRLIGRSLRA
                     GINLTALGERAVTIDCDVLQADGGTRTAAITGGFVALALACNRLVATKQLKASPITSR
                     IAAVSVGIIAREPRLDLDYREDSSAEVDCNIVMNARGELVEIQASAEGRPYTREQLSL
                     MLDLAQPGIKMLVELQQQAIDTA"
     gene            complement(5970..7310)
                     /locus_tag="VFV93_10170"
     CDS             complement(5970..7310)
                     /locus_tag="VFV93_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX5165751.1"
                     /translation="MDLFEANRQSRLRQHGPLAARMRPASLDEFVGQEAILAPGSVLR
                     RAIERDRLSSIILWGPPGVGKTTLARLIATQTKAHFVPVSAVSSGVADLRAAVKDAGG
                     RLGMHGQRTVLFIDEIHRFNKAQQDAILPYVEDGTVILIGATTENPSFEVNAPLLSRS
                     RVVVLNSLGDEQIREIILRGLNDHERGLGADELTIADDALDALVNLSNGDARFALNTL
                     EMASVAVEDGDSTITAEIVQEAAQRRAAAYDKSGESHFDTISALHKSLRDSDPDASLY
                     WLARMIERGDDPLYVARRLVRFATEDVGLADPQALTLAMAAQQAVHFLGLPEGKLALA
                     ELTVYLALAPKSNSIYRAYGAVQRDVEGTRNDPVPHHLRNAVTGLMRDIGYGKDYEYA
                     HDTEQGVSAQTHLPDNLTGRRWYEPTKRGFEATLRERLEKLHAYVAEQRNRKRP"
     gene            complement(7297..7479)
                     /locus_tag="VFV93_10175"
     CDS             complement(7297..7479)
                     /locus_tag="VFV93_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165752.1"
                     /translation="MPRKIQRQPDREHEALLLLDRLEELREDMEELGIRTIDELRQRI
                     VELEARLEDGDDDGSV"
     gene            complement(7496..8389)
                     /locus_tag="VFV93_10180"
     CDS             complement(7496..8389)
                     /locus_tag="VFV93_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165753.1"
                     /translation="MALDGDASGQQRVTSGNRRGQTPEAIDVVQSRALQLAGAAAAVT
                     LLLALYGRSLDLLRTDIPFRASNGRYYWFAVIVASIALGLVAQRLSWHDQLLANVQHR
                     APLTVAYGAAILPGLVMFAALQFVGWDSRAVIVVAAPLLAGAGVFTAAVVRHYLLTGD
                     AATLPGARLVHLALTTGVAFLSLSLTRNWMAGPLYTLVVATIIAGLLLVQAFDGVRAY
                     PIRRVAYALAGACVLGQAAYALSYWPPSGWYDGAMLTVCFAVILLVVDAILTRRISTD
                     IVARYAGAGVGVCGLLWVIAR"
     gene            complement(8350..9009)
                     /locus_tag="VFV93_10185"
     CDS             complement(8350..9009)
                     /locus_tag="VFV93_10185"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5165754.1"
                     /translation="MGTKIEQTVVTEGERVASDEHIARAVDLLRRGGVVAIPTDTVYG
                     LAASLEHPLAIERLFSIKGRSGTKAIPILISDIDAFERLSVGIPDAARRLAESYWPGG
                     LTIVVTASPLVPDAVRREGPTVGLRMPNSADALAIIAGAGGALAVTSANRSGGAEARS
                     ADEVRIKLGDRVDFVVDGGPTRLSPPSTVVETTGDEIRVLRSGAVESWRLMATLQDSS
                     E"
     gene            complement(9024..10844)
                     /gene="uvrC"
                     /locus_tag="VFV93_10190"
     CDS             complement(9024..10844)
                     /gene="uvrC"
                     /locus_tag="VFV93_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX5165755.1"
                     /translation="MGTRFSDRLSALSTGPGVYIMKNIKGDVIYVGKAAALRNRVRSY
                     FQERGHGDPKTRELVSHITDFDVIRTDTASEALILENELIKRYQPYYNVRLKDNKTYP
                     YICITAEEWPRVISTRRIIRDGSRYFGPYTSAGSVHRTLDLLNRLFPYRVCDIKITGD
                     APRPCLYYHMGRCLGPCISATTKEEYDRAIEGVALFLEGRGEELIPEMQARMESLAEN
                     LEFERAAKIRDELSAIEHVLERQKIVTGKGDNADVIAVAQSVGGDAIVQVAFVRNGKV
                     LGSEHFFMSGTRVEDEPGQILSSFVTQFYEDAAVVPPELILQHPLPDDELLSPWLAER
                     RGGKVRLTVAKRGEKRQLVEMVSKSAVENLEQNRLRWLNDEQRMTAALTELSDELGLP
                     ALPRRIECFDISNLQGTNTVASMVVFEDGKPKKSDYRRFSVKTVEGQDDFASMREVVG
                     RRFKRLAAEPDTEAWARQPDLVIIDGGKGQLNAALAAMKEVGVSISTVGLAKENEEIF
                     LPGQQTPVILDRDSQALYLVQRVRDEAHRFAVSFHRQKRQKTAIKSALDELPGVGPKR
                     KKALIAAFGSVKRIREASEEQLAQVEGISPALARQIKAQL"
     gene            11126..13762
                     /gene="alaS"
                     /locus_tag="VFV93_10195"
     CDS             11126..13762
                     /gene="alaS"
                     /locus_tag="VFV93_10195"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX5165756.1"
                     /translation="MQSRDIQSIFTEFFAERGHTNVSSSSLIPPSNDPTVLLTTAGMQ
                     QMTPYFLGLETPPDVRMTSTQKCFRTVDIDEVGDETHCTFFEMLGNFSVGDYFKRESI
                     RLGWQLLTEVYGIDPGRMTVTVNPEDDETRQLWISEIGLTLDRIYDDPSNIWGPVGDS
                     GPCGPNSEIYVDRGEQYGCGEPDCGPLCERCDRHLEVWNHVFMQWFQEKDGSRRELER
                     KNIDTGMGLERISMILQDAPSMYDTDLYQPIIRRASEISGKAYKADPQSDRSMRVIAD
                     HGRAVTFLIADGVLPGNEGRGYVLRRILRRAVRHGRLLGIERPFLTEIADVVVTEFGD
                     RYPELHARRETIRRVIQNEEESFGRTLATGISRFDALADEVRRSGGTVLHGAEAFRLY
                     DTYGFPFELTAELARDAGLEIDVEAFAEALEQSRARSRAAVKGFADSARGRVPLYAAA
                     KGAPVEFAGYERVRNDATITDLFGEDSSVEQLEAGQPGEVVLDTTVFYGESGGQVGDT
                     GTISTETGVFTVHDTQRPTPTLLVHRGEVSEGFIRADQLATAEIDANRRDAIRRNHTA
                     THVLHRALRMVLGNETHQAGSLVAPDRLRFDFTSLDALGNGGLDRVTEIANAIPLQNI
                     PVIAETMPYADAIEDGAMALFGEKYGDVVRVVKIGDYSAELCGGTHVSATGEIGPIVI
                     LAESSIGSGVRRIEAVTGDAAIRHLLRAHLVTSDLSRTLHAPVEGLLEEVRQLTAQLR
                     ERDRELEQLRLALATTDIDGLIQRSVSVDGANVLATRVPADDRETMLQIGDRLRDRMR
                     SGVIVLASEIDSQPALLAMVTKDQVGRGLSAGKLIQEIAPIVGGRGGGRPELAQGGGS
                     DASKLDEALASVVTIVKRQISG"
BASE COUNT         2406 a         4735 c         4430 g         2420 t
ORIGIN      
        1 agcaggatcg ggtcgaacca accgaggtgg ttgcagatga tcagcgcacc gccacgcttc
       61 ggcacatgct ccagcccgaa gaccttcagg cggataccca ctcgtaacgc cggcaggacg
      121 aacaggcgcg tcgtaaagaa aagcgcgtgc ttcgcgcgcc ggacgcccga catatcagaa
      181 ttcagaatcg gctcgatcat tcgcagaccg cctgcatgcg cgcttcggca tgctcgacga
      241 tcaggtcggc gacctgctcg atcgtcaacg gatcggagtc aacgacgatc gcgtcgctgg
      301 ccggacgtag cggggcaacg tcgcgctcgg agtcgatcct gtcgcgccgc ttcatatcgg
      361 ccagcacgtc ttcatacggt tgcggcttgc ccatctcgac gagctgcatg cagcgtcgtc
      421 gcgcacgctc ctccggtgac gcgtcgagaa agatcttcag atccgcatcc ggcaggacga
      481 ccgtgccgat atcacggccg accatgacga cccgtcccga gcgcgcgatc cggcgttggt
      541 gttccagcag ggcagctcga accagtgcat gagccgagac agccgagaca ttctggtcga
      601 tggccgatgt acggagtgaa ttgctcagat cacgacccgc gagcagcaca tcaacttgcc
      661 ggccatcgtc aaccgacggt cggtcgatgc aaatatccat cgtcgcggcc agcgtcgcca
      721 acgatgcggc atcatccggc ttgactccgt gctccagagc aagcagcgtc agcgctcgat
      781 agaggacgcc ggtgtcgaag tagatgccgt caatcctctg ggcgacgatc tcgccgaccg
      841 tgctcttgcc cgccgccgcc ggtccgtcga tcgcgattgt catccggcgg tcgctcaccg
      901 ccacctcctg ctccatcggc tggatgctca tgtcgtggat gcttaccctg cccgcccggt
      961 cccggtcccg gtcgcgggcc tcgtggcgcg ccggtcggcc gccggtttgg cccacctggt
     1021 cgtgaaccac cgcgtggcgg accaggcggc cggtcacgtc gatcgccgga accggaacgc
     1081 gccggccgcc gctcgtcgtc acgccgtcgc gggccgccgc gttggccccg ttggtcggca
     1141 ccgcgctcgt cacgcccacg agccggtcca cgccggtcat cgtctctgcg tgccggtcgc
     1201 cgttcacccg catcatctcc tgttgggcga gacgaaccgc gccgatcgtc gctccgccgc
     1261 gcctgcggcg ggccggcgta ctgatcgtcg cggcgcggtc gcgccggccg gttcggctgc
     1321 tcaccatcgc ggcgccgggg cgggccacca cgacggtcgt cgcggcggtc tcgtccagga
     1381 ccaccggggc cgcgcgctgc ccgctccgcg ttcgactcgc gtggacggcc ggttcgcatc
     1441 cggttggggt ctggccggtc acgcctcgga cggtcggcaa ctcgctctgc gaactcgatg
     1501 tcttcgtcgg tcagatgaag cgcttcgcgc agcaccttca gttcgccatc ggtcagatcg
     1561 cggaacgtgc cgcgcgggat cgcgccgagc tgcaatggcc caacccgcgt tcggatcagc
     1621 ttgagaaccg gatagccgac ctcgtcgaac atcttgcgaa cgatccggtt gcggccctcg
     1681 tgaatgacga tccggacgat cgtgccgtcc tccgtgtttc gaatcggccg gatcatctcc
     1741 ggcgacgtgc gctgaccatc gatcgtgatg ccacgccgga gcctgtcaag cacctccggc
     1801 ggcgggtagc catccagcag cgcctcatac tccttgtcaa tctcgtaacg ggggtgcatg
     1861 acacgattgg cgacttcgcc atcattcgtc agcagcagga gcccttcggt ctgtcgatcg
     1921 agccgtccca ccggaacgac gcgctcggcg accggcacga ggtccatgac cgtgtagcgg
     1981 ctgcgctcgt cggatgtcgt tgtgatgtaa ccgctgggct tgttcagcag gatgtaacgg
     2041 cgtcggacgg tcgggatcag cttgccgtcg acctcgatct tctgggcgcc aggctcgact
     2101 ttcgtgccaa gctcggtgac gatctcgcca tcgacccgaa cacgaccctc aaggatcagt
     2161 tcttccgcgc gccttcgtga cgcgatgccg gccgaggcaa ggactcgttg caatcgttcc
     2221 acgtcggggt cctcccggat ctatgccgcg tccggcccga tcgccccgct gcccacggag
     2281 tatatcgaca ttcgcgccac gacacgctct cccagcttga agacgacggc gccacgccgc
     2341 tcgccgcgtg gctcgctgtc gatcacgatc tcccaggtga cctgctcggc ctgcccgacc
     2401 gtcatcagca ccgtccgccg gttcaggaat gtcaatccct gtgccgccag cgcgtcgctt
     2461 gccccttgcg aggtcgcgcc aatccccagc gccagccagc gataggtccc gtcaacagcg
     2521 tccatcaccg cctgggtgtc ggcatagcga tcggcgctcc cgaggacgac gctgatcagc
     2581 tgattgtcgc cgcgccagaa gccggtgatg aggcactgcc cggcgttctc ttctgtcccg
     2641 gtcttgatgc cgaagacgtc gtcgcgggtg atgaactggt tcgagctaac cagctcgacc
     2701 tggcgggcat tcggcccgcc gatgtcggca acgtagctgt ccattgccac gatccgtgcc
     2761 agcaaccagt tcttcagcag cgcgtccgtc gcccgcacca gatcgcgggc cgaggcaacg
     2821 ttctgcgggt cgtcggtccc gacggggttc gtgaagtgcg aattcatcat cttgtgggcg
     2881 gcggcccagc tattcatctc ggcgacgaag cggtcgaccg ggtcagccgt gcccggttcg
     2941 agtcgattcc ccgcctcacg cgccagcgcc atcgcggcgt ctgcgccaga cggaatcagc
     3001 aggccatgca gcaacgtccg tacatcgacg acgtcgccag cctggagctg catgttggag
     3061 aagacggtcg ggtcgaggat gtccgattcc tggatcgtca gctgctcatc caacccaaga
     3121 atcgacgagg ccacctgagc agtgacgatc ttcaacgtgc tggccggcgg cagcggcgcg
     3181 tcagcgttgc tgctgtagat ctcaaccccg gtcgtcaggt caacgaccag cacggacgca
     3241 gcgctcaccg atggtggggc aacctgtcgc acgagcgaac cggtcgctgc aagatcgcga
     3301 ccccccgcaa cggccggcgc ccacccggac acgccaaccg ccagcaggac cgcgatctgg
     3361 agcacgacgg caattcgcat ctgcacgtgt cgattcagct tcggtatcct ctcgcgtcat
     3421 cggcggcatg ctgcctgttg aagtataggg agccgagcag caacattgcc cgacggcagg
     3481 ttgcggggta cgcttccggt caggagacga gaggagacac aggtacgatc atgagcgtcc
     3541 cgcccatgct ccgtcgcatc gtccgcgagt cggtcgaaca actgagtacc taccacccgg
     3601 tcgaagcgcc cgaggagctg gcagccaggc tggggatgcc gatcgacgcc atcctgaagc
     3661 tcgactcgaa cgagaacccg tatggctgct cgttccgtgt ccaggaggcg ctggcgacct
     3721 tcgaccgcta tcatcactac cccgacgcgc aggcacgctc cacccgcgag cggatcgccg
     3781 cctacgccgg cacaagcgtg gagcggctga tcatcggcaa tggggcggac gagctgatcg
     3841 acctgatctt cctggcgacc ctcgatcccg gcgacgaagt cgtcatccca gtcccgacct
     3901 tcggcgtcta ccgcgcccgc gccgaactgt tcggcggcgc cgccgtcgag gttccgcgcc
     3961 tgcccgactt cgatctggac atggacgcgc tgctggcagc catcaccgac cggacgaagc
     4021 tgatcatcat cacctcaccg aacaacccga ccggcaatct ggcaactaat cagcagatcg
     4081 ttcgtttgct ccagaccggc gtgctggtgg tgctcgacga agcctatttc gagatgtccg
     4141 gcaagacgtc gctgccgctg gtcggcgagt ttgacaatct ggcgatcctg cgcacgttct
     4201 ccaagtgggc cggtctggcc gggatgcgtg tcggctacgg gatcttcccg catttcctgg
     4261 ccgagcagat ctggaaggtc aagccgccgt tcaacgtcag tcaggccggt ctggtcgccg
     4321 tcgaggcgtc gctggacgac agcgagtacc tgcatggcac gctcaaccgc attcgcgccg
     4381 agcgcagccg gctgttccgc aagctgcgcc ggatcgagta cctgaacccg taccccagcc
     4441 agggcaacta cttcctctgc cgggtgacgc gcggcgacgc acacgacatc cacctgcggc
     4501 tggccgatcg cggcatcatg gtccgcgcct acggcgaccc gctcctgcgc gactgcctgc
     4561 gcatcactgt tggcaagccg gaggacaccg accggctgat cgccgcgctc cagactatcg
     4621 gcgcccatgt ctgagcgccg ggcaacgatc gctcgcgcga cggccgagac acgcattcat
     4681 ctcagcttca gtatcgacgg cagcggcacg agccgaatca acaccggcgt cggcgccctc
     4741 gatcatttcc tgactctctt cgcccgccac ggcctgttcg acctcgacct tgatgttgac
     4801 ggagatctcg ccgtcgatac ccaccacacc gtcgaggaca ccgccatctg cctcggccag
     4861 gcggtccgcg aggcagtcgg cgaccatcgc ggcatccggc ggaccgccaa cgtcgccgtc
     4921 ccgatggacg aagcgctggc gcacgtcgcc gttgactgcg gtggccggtc ctcttacgtc
     4981 cagaacggca gcttcagtgc cccggccgtt ggccagctcg aaaccgacct cgtccgccac
     5041 ttcttcgact cgctcgcccg cgaagcacgc atcaacatcc acgtcatcac gctctacggc
     5101 agcaacgccc accacgaggt cgaagccgtc ttcaaagcct tcgctcgcgc cctcgacgcc
     5161 gcgacaaccc tcgaccaccg cctcggcgac cgcctgccca gcacgaaaga acacatcgaa
     5221 cgttcgtgac gcgtgacgca tcacgcatca cgcggtatcg atcgcctgct gctgcaactc
     5281 caccaacatc ttgatgcctg gttgagccag gtcgagcatc aggctgagtt gctcgcgggt
     5341 gtagggacgg ccctcggcgc tggcctgaat ctcgactagc tcgccgcgcg cgttcatgac
     5401 gatgttgcag tcgacttcgg cggatgagtc ctcgcggtag tcgaggtcga gccgcggttc
     5461 gcgggcgatg ataccgacgc tgacggcggc aatccgcgac gtgatcgggc ttgccttgag
     5521 ctgctttgtt gccaccagcc ggttacaggc cagcgccaga gcgacgaagc cgccggtgat
     5581 ggcggctgtt cgcgtgcctc cgtcggcttg cagcacatcg cagtcgatcg tgacggcccg
     5641 ctcgccgagt gccgtcaggt tgatgccggc ccgtagcgag cggccgatca ggcgggagat
     5701 ctcctgcgat cgaccggagt ttgtggccct gtctcggttt gtgcgcgtgt gggttgcgcg
     5761 cgggagcatc gcgtattcgg ccgttaccca gccgctgcgc ttgccgacta gccagggcgg
     5821 gacacgctcc tcgatcgagg ccgtacacca gacgcgggtc gtgccgaacg cgatcagcgc
     5881 cgacccttcg gcgtagggcg cgacgccggt ttcaatcgtg actggccgca tctcgtgcgc
     5941 ctcgcggccg acgcgttgct tcgtgatgct catggtcgct tccgattccg ttgctcggca
     6001 acgtaggcat gcagcttctc aagccgctcg cgcagggttg cctcgaagcc acgcttggtt
     6061 ggttcgtacc agcgacgtcc tgtcagattg tcaggcaggt gggtctgggc gctgacgccc
     6121 tgctcggtgt cgtgggcgta ctcgtagtcc ttgccatagc cgatgtcgcg catcaggccg
     6181 gtgacggcgt tgcgcaggtg atgggggacc ggatcattgc gggttccttc gacatcgcgc
     6241 tgaaccgcgc catatgcccg gtagatcgag ttgctcttcg gcgccagcgc caggtagacg
     6301 gtcagctcgg ctaatgccag cttcccttcc ggcaatccca ggaagtggac cgcttgttgg
     6361 gccgccatcg ccagcgtcag cgcctgagga tcggccagcc caacgtcttc ggtcgcgaag
     6421 cgcaccagcc ggcgcgcgac gtagagtgga tcgtcgccgc gctcgatcat ccgcgccagc
     6481 cagtagagcg aggcgtccgg gtcggagtca cgcagcgact tgtgcagcgc cgagatcgtg
     6541 tcgaagtggg attcacccga cttgtcgtag gctgctgccc gccgttgggc tgcctcctgg
     6601 acgatctcgg ccgtgatcgt tgagtcgccg tcttcaacgg ccacgctggc catctcaagc
     6661 gtgttcaatg cgaagcgggc gtcgccattc gacaggttca cgagcgcgtc cagcgcgtcg
     6721 tcggcgattg tcaattcgtc agcgccgagc ccgcgctcgt gatcgttgag cccgcgcagg
     6781 atgatctcgc gaatctgctc gtcgccaagc gagttgagca cgacgacccg cgagcgcgag
     6841 agtagcggcg cattgacttc gaaggacggg ttctcggtcg tcgcaccgat caggatgacg
     6901 gtgccgtcct caacgtaggg caggatcgcg tcctgctggg ccttgttgaa gcggtggatc
     6961 tcgtcgatga agaggaccgt gcgttgtccg tgcatgccga gccggccgcc ggcatccttg
     7021 acggccgccc gcaggtcggc cacgccggac gagacggccg agaccggcac gaagtgcgcc
     7081 ttcgtctgcg tcgcgatcaa gcgcgcgagc gtcgtcttgc cgacgcccgg cggaccccag
     7141 aggatgatcg acgatagccg gtcacgctcg atcgcccgcc gcagaacgct gccgggcgcc
     7201 aggatcgcct cctgaccgac gaactcatcc agagacgccg gccgcatccg ggcggccagt
     7261 ggtccgtgct ggcgcagccg cgattggcga ttggcctcaa acagatccat cgtcgtcccc
     7321 atcttcgagt cgcgcttcaa gctcgacgat ccgctgacgc aactcatcaa tcgtgcggat
     7381 gccgagctct tccatgtcct cacgcagctc ttctagccgg tcaagcagca ggagcgcttc
     7441 gtgttcccga tcgggctggc gttgtatctt cctcggcacg gcgtctcctg gccagtcagc
     7501 gagcgatgac ccagagtagc ccgcatactc caacgcccgc cccggcataa cgtgcgacga
     7561 tgtcggtcga gatacggcgg gtcaggatgg catcgacgac gagcagtatc accgcgaaac
     7621 agactgtcaa catcgcgcca tcgtaccagc cggacggcgg ccagtaggag agcgcgtagg
     7681 ccgcttgccc caatacacac gcacccgcca gcgcgtaggc aacgcgccgg atcgggtacg
     7741 ccctgacgcc gtcaaacgcc tggaccagca agagacctgc gatgatcgtc gcgacgacca
     7801 gtgtgtagag ggggccggcc atccagttgc gcgtcagcga cagcgacagg aacgcgacac
     7861 ctgtcgtcag cgcgagatgc accaggcgcg cgcccggcaa cgtcgccgcg tcaccagtca
     7921 gcaggtagtg gcgaacgacg gcggcggtga acaccccggc accggccagc agtggcgcgg
     7981 caacgacgat gaccgcacgg ctgtcccagc cgacgaactg aagcgccgcg aacatcacca
     8041 gccccggcag gatcgcggcg ccatacgcca ccgttagcgg cgcgcggtgc tggacattcg
     8101 caagcagctg gtcatgccaa ctcagccgct gcgcaaccag ccccagcgca atgctggcga
     8161 cgatcaccgc gaaccagtaa taacgtccat tcgacgcccg aaatgggatg tcggtacgca
     8221 acaaatcgag cgatcgtccg tacaaggcga gcagcagtgt cacagcggca gctgcccccg
     8281 caagctgcaa cgcgcgcgac tggacgacgt caatcgcctc gggtgtctgc ccgcgacgat
     8341 tgccggaggt cactcgctgc tgtcctgaag cgtcgccatc aagcgccacg actcgacggc
     8401 tccggagcgc aacacgcgga tttcgtcccc ggtcgtttcg acgactgtcg acggcggcga
     8461 gaggcgcgtc gggccgccat cgacgacgaa atcgacccgg tcgcccagct tgatgcgtac
     8521 ctcatcggcc gaacgcgcct cggcgccacc cgaacgattc gcgctggtaa cggccagcgc
     8581 gccaccggcg ccggcgataa tcgccagcgc gtcggcactg ttcggcatcc gcagaccaac
     8641 ggtcggtccc tcccggcgca cggcatccgg caccagcggc gacgcggtca cgacaatcgt
     8701 cagcccgccc ggccagtagc tctccgccag gcggcgcgcg gcgtcgggaa tgccgaccga
     8761 aagccgctcg aacgcgtcga tatcgctgat cagaatcgga atcgctttcg ttcccgagcg
     8821 tcccttgatg ctgaataggc gctcgattgc cagcggatgc tccagagaag cggccagccc
     8881 gtagaccgtg tcggtcggga ttgcgacgac gccaccacgc ctcaacagat cgacggcgcg
     8941 ggcgatgtgc tcgtcgctgg ccacacgctc cccttctgta acgactgtct gctcgatctt
     9001 cgtgcccact cacccatccc cgttcacaac tgtgctttga tctgtcgcgc cagggccggg
     9061 ctgatgcctt cgacctgcgc gagctgctcc tcactcgcct cacggatgcg cttgacgctg
     9121 ccgaaggccg cgatcagtgc cttcttgcgc tttggcccga cgcccggcaa ctcgtcgagc
     9181 gccgacttga tggccgtctt ctgacgcttc tggcggtgga acgagacggc gaagcggtgc
     9241 gcctcgtcgc ggacgcgctg aacaaggtag agcgcctgcg agtcgcggtc aaggatgacc
     9301 ggcgtctgtt ggcccggcag gaagatctcc tcgttctcct tggccagtcc aacggtcgag
     9361 atcgagacgc cgacctcctt catcgcagcc agggccgcgt tgagctgccc cttgccgccg
     9421 tcgatgatga ccaggtccgg ctgacgggcc catgcctcgg tgtccggttc ggccgcaagc
     9481 cgtttgaagc gacgaccaac gacctcgcgc atcgaggcga agtcgtcctg accttcgacc
     9541 gtcttgaccg agaagcgacg ataatcgctc ttcttcggct tgccatcctc gaaaacgacc
     9601 atcgacgcaa ccgtgttggt gccttgcagg ttggagatgt cgaaacactc aatccgtcga
     9661 ggcaaggcag gcagtcccag ttcgtcggac agctccgtca gcgcggccgt cattcgctgc
     9721 tcgtcgttga gccagcgcag ccggttctgc tccagattct cgacggcgct cttcgagacc
     9781 atctcgacca gttggcgctt ctcgccacgc ttcgcaactg tcaaccggac cttgccgccg
     9841 cgacgctcag ccagccacgg cgacagcaac tcatcatccg gcaacgggtg ttgcaagata
     9901 agctcgggcg gcacaacggc tgcgtcttcg tagaactgcg tcacgaagct gctcagaatc
     9961 tggcccggct cgtcctcgac gcgcgtgccg gacatgaaga agtgctccga gcccagcacc
    10021 ttgccgttac gaacgaacgc cacctggacg atggcatcac cgcccaccga ctgggcaacg
    10081 gcgatcacgt cggcgttatc gcccttgccg gtgacgatct tctgccgttc gaggacgtgc
    10141 tcgatcgcgc tcagttcgtc gcggatcttg gcggcccgct cgaactccag gttctcggcc
    10201 agtgactcca tccgagcctg catctcgggg atcagctcct cgccacgccc ttcgaggaag
    10261 agcgcgacgc cctcgatcgc ccggtcgtac tcctccttcg tcgtggcgct gatgcacggc
    10321 ccgaggcagc ggcccatgtg gtagtagagg cagggccggg gcgcgtcgcc ggtgatcttg
    10381 atgtcgcaga cccggtacgg aaagagccgg ttgagcaggt cgagcgtcct atggaccgaa
    10441 cccgccgagg tatatggacc gaaatagcgc gatccatcgc ggatgatgcg ccgggtcgag
    10501 atgacgcgcg gccactcctc ggccgtgatg cagatgtacg ggtacgtttt gttgtccttc
    10561 agccgaacgt tgtagtacgg ctggtagcgc ttgatcagct cgttctctag gatcagcgcc
    10621 tcgctggcgg tatcggtacg gatcacgtcg aagtcggtga tgtggctgac cagctcgcgc
    10681 gtcttcggat cgccgtgtcc tcgctcctgg aagtaggagc gcacgcgatt ccgcagggca
    10741 gccgccttcc cgacgtagat aacgtcgccc ttgatgttct tcatgatgta gacgccggga
    10801 cctgtcgaga gcgccgacag ccggtcggag aagcgcgttc ccataccctc tattttaccc
    10861 cccgcggctt cgtgtcgtcc tgaggccgca gccgaaggac ctctctgtgc tgaccgcgag
    10921 cgaactcaac acggagaggt ccttcggctg cggcctcagg acgatagaga tgctcgtttc
    10981 cttgacaggc agtcagcgta cgagcatact gtacgtacac tgcgccgaag tcggcggtgt
    11041 gccggattac aggccacgtt cgttacactt acgcgatgcg agcttcccat cgcaccacga
    11101 aatgtaagga acgggccgga ctgctatgca gagtcgtgac atccagtcga ttttcactga
    11161 gttcttcgcg gaacggggac acacgaatgt gtcctcctcg tcgctgatcc cgccgtccaa
    11221 cgacccaacc gtcctgctga cgacggccgg catgcaacag atgacgccgt acttcctcgg
    11281 tctcgaaaca cctcccgatg tccgcatgac ctcgacccag aagtgcttcc ggacggtgga
    11341 catcgacgag gtcggcgacg agacgcactg cacgttcttt gagatgctcg gcaacttctc
    11401 ggtaggcgac tacttcaagc gcgagtcgat ccgcctcggc tggcagttgc tgaccgaggt
    11461 ctacggcatc gacccaggcc gaatgacggt gacggtcaac cccgaggacg atgaaacgcg
    11521 ccaactctgg ataagcgaaa tcggcctgac cctcgatcgc atctacgacg atcccagcaa
    11581 tatctgggga ccggtcggcg atagtggacc atgcggcccg aacagcgaga tctacgtcga
    11641 tcggggcgag cagtatggct gcggcgagcc ggattgtggc ccgctgtgcg aacgctgcga
    11701 ccggcacctc gaagtctgga accacgtctt catgcagtgg ttccaggaga aagacggcag
    11761 ccgccgcgag ctggaacgca agaacatcga caccggcatg gggctggagc ggatcagcat
    11821 gatcctccag gacgccccgt cgatgtacga cacggatctc taccagccga tcatccggcg
    11881 cgcgtcagaa atttccggca aggcgtacaa ggccgacccg cagtccgacc gatcgatgcg
    11941 cgtcatcgcc gaccatggcc gggccgtcac gttcctgatc gccgacggcg tcctgccggg
    12001 caacgagggc cgcggctatg tcctgcgccg aattctgcgc cgcgccgttc gccacggcag
    12061 gttgctcggc atcgagcgcc cgttcctgac cgagattgcc gacgtcgtcg tcaccgagtt
    12121 cggcgaccgc tatccggaac tgcacgcgcg acgcgaaacg atccggcgcg tgatccagaa
    12181 cgaggaagag agcttcggac ggacgctggc gaccggcatc tcgcgattcg acgcactggc
    12241 ggacgaggtg cgccggtcgg gtggcacggt cctgcatgga gccgaagcgt tccgactcta
    12301 cgacacctac ggcttcccgt tcgagctgac ggccgagctg gcccgcgacg ccgggctgga
    12361 aatcgacgtc gaggcgtttg ccgaggcact ggagcagtcc cgggcccgca gccgggcggc
    12421 cgtcaagggc tttgccgaca gcgcgcgcgg ccgcgtgccg ctctacgcgg ctgccaaagg
    12481 cgcgccggtc gagttcgccg gctacgagcg ggttcgcaat gacgcgacga tcaccgatct
    12541 gttcggagaa gacagctccg tcgagcaact ggaagccggc cagcctggcg aggttgtgct
    12601 cgacactacc gtcttctacg gcgagtcagg cggccaggtc ggcgacactg gcacgatctc
    12661 aactgagacc ggcgtcttca ccgtccacga cactcagcgg cccacgccga cgctgttggt
    12721 ccaccgtggt gaggtgagcg aggggttcat ccgcgcggac cagttggcga cggctgagat
    12781 cgacgccaac cggcgcgacg cgatccggcg caaccacacc gccacacacg tcctgcatcg
    12841 ggcgctgcgg atggtgttgg ggaacgagac gcaccaggcc ggttcgctgg tggcacccga
    12901 ccggctgcgc tttgatttca ccagcctgga tgcgctcggg aacggtggcc tggaccgagt
    12961 gacggagatt gccaacgcga tcccgctcca gaacatcccg gtgatcgccg agacgatgcc
    13021 gtatgccgac gcgattgagg atggcgcgat ggcgctcttc ggcgagaagt acggcgatgt
    13081 cgtgcgcgtc gtcaagatcg gcgattactc ggcggagctt tgcggcggaa cgcacgtctc
    13141 cgcgacgggc gaaatcggcc cgatcgtcat cctggccgaa tcctcgatcg gctctggcgt
    13201 ccggcgcatc gaggcagtca ctggtgacgc ggcaatccgc cacctgctgc gcgcgcacct
    13261 ggtcacgagt gacctgtcac ggacgctgca tgcaccggtc gagggtctgc tcgaagaggt
    13321 gcgccagctc accgcccagc tgcgtgagcg ggaccgggag ctcgaacaat tacggctcgc
    13381 tctggcgaca acggacatag acgggctgat ccaacgttcc gtgtcggtcg atggcgcaaa
    13441 cgtgctggcg acgcgcgttc ctgccgacga ccgcgagacg atgctgcaaa tcggagaccg
    13501 gctgcgcgac aggatgcgat cgggcgtcat cgtcctcgcc agcgagatcg actcgcagcc
    13561 ggccctgctg gcgatggtga ccaaggacca ggttggacgt ggcctgagcg ccgggaagct
    13621 gattcaggag atcgcgccga tcgtcggcgg tcgcggtggc ggccggccgg agctggcgca
    13681 gggcggcggg tcggacgcga gcaagctcga tgaggcactc gcgtcggttg tgacgattgt
    13741 gaagcgacag atttccggct agagccgtct tgcggtggac tgcgtctgca catcgcgcgg
    13801 cggctcctgg aggagcggat agatggccgc gcgctatgta gaacagttca ccgtccggac
    13861 ggccgacgag cttgacgagc tggccgaggc gctcggcgac ggccagcttg aggcggtcgt
    13921 cgtcgatgtg cctgacggcg ccagcgcgtt ccagacggcc cgcgagtttc gccagatttt
    13981 gaacctggcc c
//
LOCUS       DASUGJ010000461         5480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1740731, whole genome shotgun sequence.
ACCESSION   DASUGJ010000461 DASUGJ010000000
VERSION     DASUGJ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740731"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VFV93_10200"
     CDS             <1..1033
                     /locus_tag="VFV93_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX5165757.1"
                     /translation="AGWTSTPWVAGGATTVTNGSAVVDGARLSTTGFYSPNHVLEFVA
                     TFQPGVPNQHIGFGTTLNSTPWAIYSTGGDGASLRARTHTGSVAPTAELGASWLGGPH
                     LFRIEWTASQVIYSIDGAVVATHNAAITGQMRPLASDSAAGSGTLVIDWMRLSPYQPS
                     GSFTSRVLDAGGAATWTSASWTANTPAGTTLAVSVRSGTTPVPDGSWSVFSAVSGNGA
                     SIANTGRYVQYRLDLSTTVPGVTPSLRDIILTYQNIVVTAPTVVSVTPDTGPATGGSG
                     VTIVGTGFSAGATVSFGGVAAASVIVVSGTEITAVTPEHPAGTVDVTVTNTDDATGAL
                     PGGFTYEAP"
     gene            complement(1172..2350)
                     /locus_tag="VFV93_10205"
     CDS             complement(1172..2350)
                     /locus_tag="VFV93_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165758.1"
                     /translation="MDATDAIVFVIVVLGRFAIPLGIPRYPLPSIIAAMILDAADQTI
                     FQQFTSLNLDGYQSYDKALDIYYLAIAYISTLRNWTSVSGYSAARFLWYYRLVGVTAF
                     ELTQWRPLLLIFPNTFEYFFDWFEGVRTRWNPVRLTQRHVISAAAFIWIFIKLPQEYW
                     IHIAKLDTTDLIKENILGVSTDTPWGEALSQNLWVFPVLLAVAVGLVLFFRWLRKRLP
                     EPDWKTTFDVDTHLEPIPPELMQTRRVRSIRYGLLEKIVLVGLMIVVFAQMLPNIDVN
                     NVAVVAGVAFVIAINAFVSHWFALRGTEWRSLGTELAAMAAINLAAGMLYLYLLPGSA
                     DDLDIGAPIFFALLLTLIVVLYDRYRPIHDARMQMAAATVIPPPTIAGANEPEQSVAP
                     "
     gene            2626..3453
                     /locus_tag="VFV93_10210"
     CDS             2626..3453
                     /locus_tag="VFV93_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5165759.1"
                     /translation="MTSTSVRSFEVVLGEKAGQPVSDQTESTQVTWFGHSTVLIRMGE
                     TTILTDPLLRGRAAHLRRRVPLLEEDWPAAVDAILISHLHLDHCDLPTLRRLGRHIPL
                     LVPVGAGTWLRGRGFTNVVELPVGADHVVESVVVTAVPAEHPGRRWPLRPDAETIGFL
                     VRGPKTFYFAGDTELFDGMGSLGVELDVALIPVWGWGRSLGTGHMDPARAAEALRLQQ
                     PRIAIPIHWGTLHPYGTAIRDKRFLEDPPNQFAELARSIAPSVDVRIMQPGETIVIE"
     gene            complement(3478..3666)
                     /locus_tag="VFV93_10215"
     CDS             complement(3478..3666)
                     /locus_tag="VFV93_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165760.1"
                     /translation="MFTRRQAVDVTHLQQAGFTTEEIDQLDALRQTYPLIERVDSQRE
                     FQWLTFMRWCYVTGVIGE"
     gene            complement(3757..5259)
                     /locus_tag="VFV93_10220"
     CDS             complement(3757..5259)
                     /locus_tag="VFV93_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5165761.1"
                     /translation="MASYTRATTTAWPPNPMIYEINTWVWLQTLSEASGTAVTLDSVP
                     DRAWDDLAEIGMDAVWLMGVWERSPAGREIALENPNFQRSFIEALPGYQPEDISGSPY
                     CVRQYQVDPALGGPKGLQKARQALEERGLRLILDYVPNHVAPDHPWTERHPEFFIRGS
                     DADLATDSASFVRVGDAVLACGRDPFFPAWPDVVQLNAFEPRLRQATIATLRDIASQC
                     DGVRCDMSMLLINSIFADTWGERAGNRPELEFWREVIPAVKETAPEFRFIAEAYWDLE
                     WELQQQGFDYCYDKLLYDRLVHDDATAVRGHLWADMEYQRKLLRFIENHDEPRAAATF
                     APGQLEAATVVETTLPGGCLIYDGQRDARRVRLPVLLGRQPDEPDDPVFRDFTGRLLL
                     AIQTTGMRNGDWKLCATSGWPDNQRHNQLLAWTWKGQSRRSLVVVNYASEPAQGIVHL
                     SWDDISGAHWTLTDRMSDAVYERDGHELDTEGLYVELAGWGHHVLQFSRV"
     gene            complement(5292..>5480)
                     /locus_tag="VFV93_10225"
     CDS             complement(5292..>5480)
                     /locus_tag="VFV93_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165762.1"
                     /translation="GDNGAGLGASHQTGWTGLVAPLILLQHVARAEDVAATGRLSGSA
                     RRAPTSTAAGTATHSGTG"
BASE COUNT         1020 a         1678 c         1751 g         1031 t
ORIGIN      
        1 ggctggctgg acgagcactc cctgggttgc tggtggcgcc acgacggtta ccaacggcag
       61 tgccgtcgtc gatggtgcac gcctgtccac gacgggcttc tacagcccca accacgtcct
      121 tgaattcgtc gcgaccttcc agccaggtgt gcccaaccag cacatcgggt tcggcacgac
      181 gctcaacagc acaccatggg cgatctacag caccggcggt gatggagcca gcctgcgggc
      241 gcgcacgcac accggctcgg tggcaccgac tgccgaactg ggcgccagct ggctcggcgg
      301 tccgcacctg ttccggatcg agtggacggc tagccaggtc atctactcca tcgacggagc
      361 ggttgtggcc acccacaacg ctgccatcac cgggcagatg cggccgctcg ccagtgacag
      421 cgccgctggt agcggcacgc tcgtcatcga ctggatgcgg ctgagtccgt accagccatc
      481 gggcagcttt acctcacggg tcctggatgc cggtggtgca gccacctgga cgagtgccag
      541 ctggaccgcc aacacgccgg ccgggacgac gctggcggtc agtgtccgta gtggcacgac
      601 gccagtcccg gatggcagct ggagcgtctt cagcgccgtc agcggcaatg gggccagcat
      661 cgccaacaca gggcgctacg tccaataccg tctcgatctg tcgacgaccg taccgggcgt
      721 gaccccatcc ctccgtgaca tcatcctgac atatcagaat atcgtggtta ccgcgccgac
      781 cgtcgtcagc gttacacctg ataccggccc ggcgacaggt ggctccggag tcacaatcgt
      841 cggtaccgga ttctccgcgg gagcaacagt cagcttcggg ggtgtagcgg ctgccagtgt
      901 catcgtcgtc tcgggcactg agatcacggc cgtcacacca gaacatccag ccggcacagt
      961 tgatgtcacc gtcacaaaca cggatgacgc gactggtgcg ctaccgggag gatttaccta
     1021 tgaagccccg tgatgtcgtg agttgcccat cggctgtgtc gaagagatga cgcaatggca
     1081 acggcggtga gttagaagac tcaccgccgt tgtcattgcg tggatatcga gatgcggcct
     1141 ggtgcgaatt gctcgttcgc tccgtatgcg cctacggtgc aaccgattgc tccggctcgt
     1201 tcgctccggc gatcgtcggc ggaggaatga ccgttgccgc cgccatttgc atgcgggcgt
     1261 cgtggattgg ccggtagcgg tcgtacagga cgacgatcag cgtcagcagc aaggcaaaga
     1321 aaatcggcgc gccgatgtcg aggtcgtccg cgctccccgg taacaggtag agatacagca
     1381 ttccggcggc caggttgatc gcggccatcg ctgcgagctc cgttccaagt gagcgccatt
     1441 cagtcccgcg cagcgcgaac cagtggctca cgaacgcgtt gatagcgatg acgaatgcga
     1501 ctcctgccac gaccgcgacg ttattgacat cgatgttggg cagcatctgc gcgaagacga
     1561 cgatcatgag tccgaccagg acgatcttct caagcagtcc atacctgatc gagcgcacac
     1621 gacgcgtctg catgagttcg ggcgggattg gttcgaggtg cgtatcgaca tcgaaggttg
     1681 tcttccagtc cggctcgggc aagcgcttgc ggagccatcg aaagaagagc accagaccga
     1741 ctgcgacagc cagcaggacc gggaagaccc agaggttctg gctcagcgct tctccccacg
     1801 gtgtgtcggt cgagactccg aggatgttct ccttgatcag gtcagttgtg tcgagcttgg
     1861 cgatgtggat ccagtactcc tgtggcagct tgatgaagat ccagatgaac gcagcagcgc
     1921 tgataacgtg tcgctgtgtc agccggacag gattccagcg tgtgcgcacc ccttcgaacc
     1981 agtcgaagaa gtactcgaac gtgttgggga agatgagaag caagggtcgc cactgcgtca
     2041 gctcgaaggc ggtgacgccg accaggcggt agtaccacag gaaccgggcg gcagagtagc
     2101 cagatacgct ggtccagttg cgcagtgtcg agatgtaggc gatggcgagg tagtagatgt
     2161 ccagtgcctt gtcgtacgac tggtagccgt caaggttcag actggtgaat tgctgaaaga
     2221 tcgtctgatc cgcagcgtcg aggatcattg ctgcgatgat gctcggaagc ggatagcgcg
     2281 ggatgccaag cggaatcgca aacctcccga gaacgacgat cacaaagacg atagcgtcgg
     2341 tcgcgtccat gttcctcgtt ccacttctca gtcgccccgc gcatgaaaag gcattcgcag
     2401 gcggttgtag cgcgcgatgg tcgcaatgaa ctcgatccca tcgtatgccc accatcctgt
     2461 aagaagtaca gtccagaggg cgcaatcgag aggtgcagtt ggctggtggt ctcggagtga
     2521 tcctgcgagg gacaggtacg accggagaat gcgtcgaagc gggtgatcct gagcccgcag
     2581 aaagcgacta tcgttgtcag cgggctcagt tcgccgtcga gttcgatgac atccacctcc
     2641 gtgcgttcgt tcgaagtcgt cctcggtgag aaagccggcc agcccgtgag cgatcagacg
     2701 gagtcaacac aggtcacgtg gtttggacac tcgaccgtcc tgatcaggat gggggagacc
     2761 acgattctga ccgatccgct gctgcgtggc agggcggccc atctgcgacg ccgcgtcccg
     2821 ctcctggagg aggactggcc tgccgcagtc gacgcgattc tcatctcgca tctacacctc
     2881 gatcattgcg acctgccgac gctgcggcgg ctcggacgac acatcccgct gctggttccg
     2941 gtcggagcag ggacgtggtt gcgggggcgc ggcttcacga acgtcgtcga gctgccggtc
     3001 ggcgctgatc acgtcgtcga gagcgtcgtt gtgaccgccg taccggccga acatccggga
     3061 cgtcgctggc cgctacggcc ggatgcggaa acgatcggct tcctcgttcg tggtccgaag
     3121 acgttctact tcgcgggtga cacggaactg tttgacggga tgggctcgct cggcgttgag
     3181 ctcgacgtcg cgctcatccc ggtctggggt tggggccggt cgcttggtac cggtcatatg
     3241 gatccggcac gggcagccga agcgctgcga ttgcaacagc cacgaatcgc gataccgatt
     3301 cactggggaa ccttgcaccc atatggaacg gcaattcgcg acaaacggtt tcttgaggat
     3361 ccccctaacc agttcgcgga gttggctcgc tcgatcgctc cgtcggttga cgtcaggatc
     3421 atgcagccgg gcgagacgat cgtgatcgag tagcgacggc ggttgtatgg tgtcgcctca
     3481 ctcaccgata acgcccgtga catagcacca gcgcatgaat gtgagccatt ggaattcgcg
     3541 ctgtgaatcg actctctcga tcagggggta ggtctgacgc aacgcatcca actggtcgat
     3601 ttcttccgtc gtgaagccgg cctgctgcag gtgcgtcacg tcgacggcct gtcgccgcgt
     3661 gaacatcgcg atcctcatcg ctgttgctcc tgattccaga ccgactgcgc caggcggcga
     3721 caggcatctc gctacaacaa tcgactcgcc ggatgtctac actctcgaga actgcaggac
     3781 atgatgtccc cagcctgcta actcgacgta cagcccttcg gtgtccagct cgtgaccgtc
     3841 tcgctcatag acggcatcac tcattcggtc ggtcaatgtc cagtgcgcgc cggagatgtc
     3901 gtcccacgac aaatggacga tgccctgggc aggttcgctg gcatagttca cgacgacgag
     3961 cgagcgtcgg gactgaccct tccacgtcca ggccaggagc tggttgtgcc gttggttgtc
     4021 cggccagccg ctggtcgcgc acagcttcca gtcgccgttc cgcataccgg tggtctggat
     4081 tgcgagcagg agccggccgg taaagtcccg gaacaccggg tcgtcgggtt catcgggctg
     4141 gcggccgagc agtaccggca gccggacgcg tcgcgcatca cgctgaccat cgtagatgag
     4201 gcaaccgccc ggcaacgtcg tctcgacgac agtcgccgcc tcgagctggc caggagcgaa
     4261 cgtcgccgcc gctcgtggtt catcgtgatt ctcgatgaat ctgagcagct tgcgctggta
     4321 ctccatgtcg gcccacaggt gcccacggac ggcggttgcg tcgtcgtgta ccagccggtc
     4381 gtagaggagc ttgtcgtagc agtagtcaaa cccctgctgt tggagctccc attcgagatc
     4441 ccaatacgcc tcggcaatga agcggaactc gggcgcggtc tccttgacgg ctggaatgac
     4501 ctcgcgccag aactcaagct ccggccggtt gccggcgcgt tcgccccagg tgtcggcaaa
     4561 gatggagttg atcaacagca tcgacatgtc gcagcggacg ccgtcgcact gggacgcgat
     4621 atcgcgcagt gtcgcgatcg tcgcctgacg cagccgcggc tcgaaggcgt tgagttgaac
     4681 gacgtcgggc caggccggga agaatgggtc gcggccacat gcgagcaccg cgtcaccgac
     4741 gcgcacaaag ctcgcggagt ctgtcgcaag gtctgcatca cttcctcgga tgaagaattc
     4801 cggatggcgc tccgtccagg gatgatcggg cgcgacgtga tttggaacgt agtcgaggat
     4861 cagacgaagg ccccgttctt cgagagcctg acgcgccttc tgcagaccct tcggccctcc
     4921 gagggccgga tcgacctggt attgacgcac gcaatagggc gacccggata tatcttcagg
     4981 ttggtaaccg ggtagggcct cgatgaacga ccgttggaag ttcggattct ctaacgcgat
     5041 ctcgcggccg gccgggctgc gctcccagac gcccatcaac cagactgcat ccatcccgat
     5101 ctcagccaga tcgtcccagg cacggtccgg cacggagtcc aaggtgactg ctgtgccgga
     5161 cgcctcgctc agtgtctgca accagaccca ggtattgatc tcgtagatca ttgggttcgg
     5221 cggccatgct gtcgtggtcg cacgggtgta gcttgccatc actcgctcct ctcaggtggt
     5281 cagcacggcg actaaccggt tccggaatgt gtggccgttc ccgctgccgt gctggttggg
     5341 gcacggcgcg cggagccgct cagtcgcccc gttgcggcaa catcttccgc ccgggcgacg
     5401 tgctggagca ggatcagcgg cgcgaccagg ccggtccagc cggtctggtg gctggcgccg
     5461 agtccggcac cgttgtcgcc
//
LOCUS       DASUGJ010000462         5984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_767763, whole genome shotgun sequence.
ACCESSION   DASUGJ010000462 DASUGJ010000000
VERSION     DASUGJ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767763"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1287)
                     /locus_tag="VFV93_10230"
     CDS             complement(85..1287)
                     /locus_tag="VFV93_10230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165763.1"
                     /translation="MIGTESTAPAVATAAPIGSIRRFYLFTLLTRTEFTGGIWILYLK
                     SRGLSLTEIGLSEAAFHLAPVLFELPSGSFADLVGRRWSLAIGSLLIAISTALMFIAN
                     SLPVAMLALFLNGASYSFRSGADQAYLYDSLGDRQGSFAGILGRLFGATYIVGGATLW
                     LGAALSDISYTWPYGIAIALALAGTWLAAGLAEPPASTRGEGHSSVLHHLRAVRTVLR
                     ANPPVAAMLVYSGTFWAAGTVAFLYLQAAFSDRGLSNSTVGLIIGGALVLNALGATFA
                     GHFEQRGRFRNQLVVLALLTGLGLAATAAGPLGIATTAYLLSNLFSGLLEPLMFTWFN
                     RHVPSGQRATLLSVESWMFSLTMIPAFPLGGWLAETHGWGALLLVCGGAKITLTLLVA
                     VGSRLRVA"
     gene            complement(1546..2529)
                     /locus_tag="VFV93_10235"
     CDS             complement(1546..2529)
                     /locus_tag="VFV93_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5165764.1"
                     /translation="MSEPMTGPTAAQPSATLRRADGSQRRWVGAACLLGAALIWGFVP
                     TSTRYVVQTLSPGNILLARFLVGAVAAVILFSLFKAPLPPRKLIPHAVALGLLGQLGF
                     NVPLAYGIQHVEAGTAALISGLSSVFIAVLAVPVLGERLQGRVVVGLLLALSGSVVVS
                     IMSGGDVSLSRDQAFGSLLVLLSATLWAVYSVFVKPWLGRIPPTSIPMLGSIAGLPLM
                     LPLGASGMREGLGDLTWTGWLGVAQFTVAASVVAPILWAVGLQRGEASRAGLYLYLTP
                     LFGVIISAILLKEPVTLGTIAGGGLIVAGVVLATLPALGRQRLGARAAGRS"
     gene            complement(2545..4254)
                     /locus_tag="VFV93_10240"
     CDS             complement(2545..4254)
                     /locus_tag="VFV93_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5165765.1"
                     /translation="MLTHQGTRVRRRAADAVVDALRAEGVEVVFGLVGSHVISVYDAL
                     ADAPEIKHITVKHETTASGMADAYGRLTGKPGVVLVTAGPGATNSLTGVAQAYMAASP
                     MVHISGAVPTNASFESFHGVDKPDFLVRMFAEVTKWSVVVERVEDIPGVLSRAFAIAT
                     SGRPGPVHIEIPQDVITAGETELDVYQPTEIAPVSLDPASLASLTSLIAGSSKPVIWA
                     GKGVRATMADTELAELAELLEAPVILAGDAAGAFPDTHPLSTGQLSLYERTPLQRELA
                     GEADLILAIGERGGTGHADALFDVAAGTPVAGIWLGNDGERADARATGGAVADIRTGL
                     NQLIEASGDQQRPVDATLRARIKKGKDGLRTHIMDGVMRQFGDSNPMHYGAALAALAE
                     LVDEDTICLGDIGSHNQWTRMVVQTLNRTTFTPEGYWGAMGFGLPAAMAAKIVAPDKK
                     VISVTGDGCFLMASSDFATATEYGLNPVIVILNDRQYGMIVGMQQGNYGRTYQTELNG
                     PDFVAFAKSFGGDGIRVDRPEQMREALERGFASPTIFVIDATCTHEVPNYNMRAAWAE
                     LSQ"
     gene            complement(4375..4590)
                     /locus_tag="VFV93_10245"
     CDS             complement(4375..4590)
                     /locus_tag="VFV93_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165766.1"
                     /translation="MSLPLPILHASGFSWDEALVLVIAILAVPAISFFTGRFGKRRAS
                     ESASRKRHHVSEPAADDAVSDPTIDQL"
     gene            4782..5039
                     /locus_tag="VFV93_10250"
     CDS             4782..5039
                     /locus_tag="VFV93_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165767.1"
                     /translation="MDDEQRGGSMPGFMIPIGPIILIAIVFMVLRSRRKSKDERAFAR
                     IVNAIDDSELPDRAKDLLRQSVDEVKGAMASIREMAGELRS"
     gene            complement(5036..>5984)
                     /locus_tag="VFV93_10255"
     CDS             complement(5036..>5984)
                     /locus_tag="VFV93_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5165768.1"
                     /translation="LAVADGPSERRCGHATHTGVESMERMTASHSGRVPDYVAIGHMT
                     IDRTPSGDMLGGSVLYAALTAARYGLRAAILTRANLAALDGQQRRQLDEIAAEVEIVT
                     QASNGITTFTNTEVAGRRSQQIHDWGGEIDLTGLPPLWRSAGAIHLAPVAQEIEPRQV
                     SRLSPRLLGCTPQGWMRRWDEERLGKVRAVPLRLPADLISRIDALVVSAEEYTHSRDI
                     VVQVGERGLSAITRGMQGASLIDRGREVDIPAYRIKVVDTTGAGDVFAAALFAARSER
                     QSVSASARYASAAAALNVSGDGLSSVPRRAEVEALIDRS"
BASE COUNT         1040 a         1927 c         2062 g          955 t
ORIGIN      
        1 aaggacctct ccgccatagc accacacgag gttgaagcgg agaggtcctt cgctgtggct
       61 caggacgacg gggaacgtgt tgcgtcaggc gacccgcagg cgcgagccga cggcgaccag
      121 tagtgtcagc gtgatcttgg cgccgccgca gacgagcagg agggcgcccc agccgtgagt
      181 ctcggcgagc cagccgccga gtggaaaggc ggggatcatc gtcagcgaaa acatccacga
      241 ttcgaccgac aggagtgtcg cccgctggcc ggacggcaca tggcggttga accaggtaaa
      301 catcaacggc tccagcaggc cgctgaacag gttcgagagc aggtaggccg tcgtcgcgat
      361 gccgagcggg ccggcggcgg ttgcggccag tcccagtccg gtcagcagcg ccagcacgac
      421 gagctggttg cggaagcgcc cgcgttgctc gaagtggccg gcgaacgtcg cgccgagtgc
      481 gttgagcacc agcgctccgc cgataatcag cccgacggtc gagttggata gcccccgatc
      541 ggagaacgcc gcctggaggt atagaaaggc cactgtcccg gctgcccaga aggtgccgga
      601 gtagaccagc atcgccgcga ccggcgggtt ggcgcgcagg accgtccgga cggcgcggag
      661 gtggtgaagg actgacgagt gaccctcgcc gcgcgtagac gccggtggct cggccaggcc
      721 tgccgccagc caggttccgg ccagtgccag cgcgatcgcg atgccgtacg gccaggtgta
      781 ggagatatcc gagagagccg cgccgagcca gagcgtcgcg ccgccgacga tgtacgtcgc
      841 gccgaacagc cggccgagga tgccggcgaa gctcccctgc cggtcgccta gtgagtcgta
      901 gaggtaggcc tgatcggctc cggagcggaa cgagtaggac gcgccgttca ggaagagcgc
      961 cagcatcgcc accggcaacg agttggcgat gaacatgagc gccgttgaaa tcgcgatcaa
     1021 cagcgagccg atcgccagcg accagcgccg tccaaccaga tcagcgaatg atccggacgg
     1081 tagctcgaag aggaccgggg cgaggtggaa ggccgcttcg gacaggccga tctcggtcag
     1141 gctcaggccg cgactcttga gatagagaat ccagatgccg ccggtgaact ctgttcgggt
     1201 cagcaatgtg aagagataaa agcgacgaat gctgccgatt ggggcggccg ttgccaccgc
     1261 cggcgcggtt gattcagtgc caatcatcgg tgaggtgctc ccacgtatcg tggggagggg
     1321 gtgtctgcgc cgggttcacg cccggccaca tgacgacgcc cgcccccgct gtccgcctgc
     1381 gtcgagtgtc aggacagcgg gacgttgtgc gttgtcgtgt tcttccagat gaaccagtcg
     1441 cgcgattcgt ctcgcgcggc ctgtttgcat tccatcaacg gtggctctcg ctccgtcttc
     1501 cgcgaagaca acgtcgcgga tgatacaccg cggtcgtcgc cgctgtcacg atcgaccggc
     1561 ggcacgtgcg cccagtcgct ggcgtccgag cgccggaagc gtcgccagga cgacaccggc
     1621 gacgattagt ccgccgcccg cgatcgttcc gagtgtgacc ggctctttga gcaggatggc
     1681 gctgatgatg acgccgaaga gcggtgtcag gtagaggtat agtcctgccc gcgatgcctc
     1741 accccgctgg agaccgaccg cccagaggat cggagcgacg acgctggccg ccaccgtgaa
     1801 ctgcgcgacg ccgagccagc cggtccaggt cagatcgccg agaccttcgc gcatgccgga
     1861 agcgcctagc ggcagcatca gcggcagacc ggcgatcgag cccagcatcg ggatgctggt
     1921 cggcgggatc cggcccagcc agggcttgac gaaaacggag tagaccgccc aaagcgtcgc
     1981 cgagagcagg accagcaggc tgccgaacgc ctgatcgcgc gagagcgaca cgtcgccgcc
     2041 actcatgatc gacacgacga cactccccga cagcgccagc agcagcccga cgacaacccg
     2101 tccctgtaat cgctcaccaa ggaccggcac ggccaggacg gcgatgaaga ccgacgagag
     2161 ccctgagatc agggcggctg tcccggcctc gacgtgctgg atcccgtagg cgagcgggac
     2221 gttgaagccg agctgcccca acagcccgag ggcgacggcg tgtgggatga gcttgcgcgg
     2281 tggcagcggt gccttgaaca ggctgaacag gatcaccgcc gccaccgcgc cgaccaggaa
     2341 gcgggccagc aggatgttgc ccggcgacag cgtctggacg acataccgcg tcgaggtcgg
     2401 gacgaagccc cagatcagcg ctgccccgag caggcaggct gctccgaccc aacggcgttg
     2461 actgccgtcg gcgcgacgca acgtggcgga cggttgcgca gcggttgggc cggtcatcgg
     2521 ctcgctcaca tctcaggacg cgccttactg gctcagctcc gcccaggctg cgcgcatgtt
     2581 gtagttcggc acctcgtggg tgcaggtcgc gtcgatgacg aagatcgtcg ggctggcgaa
     2641 tccgcgttcc aacgcctcgc gcatctgctc aggccggtcg acccggatac catcgccgcc
     2701 gaacgatttc gcgaaggcga cgaagtcggg gccattcagt tcggtctggt acgtgcgacc
     2761 atagttaccc tgctgcatgc cgacgatcat gccgtactgc cggtcgttca ggatgacgat
     2821 gaccgggttg aggccgtact ccgtcgcggt cgcgaagtcg ctgctggcca tcaggaagca
     2881 gccgtcgccg gtgacgctga tgaccttctt gtccggcgcg acgatcttgg ccgccatcgc
     2941 cgccggtagg ccgaagccca tcgcgcccca gtagccctcg ggcgtgaagg tggtgcggtt
     3001 cagcgtctgg acgaccatcc gcgtccactg gttgtggctg ccgatgtcgc cgaggcagat
     3061 cgtgtcctca tcgaccagct cggccagggc tgccagcgcc gcgccgtagt gcatcgggtt
     3121 gctgtcgccg aactggcgca tgacgccgtc catgatgtgt gtgcgtagtc cgtccttgcc
     3181 cttcttgatt ctggctcgca gcgtggcgtc gaccggccgt tgctggtcgc cactggcctc
     3241 gatgagctgg ttcaggccgg ttcggatatc tgcgacggct ccgccagtcg cacgcgcatc
     3301 ggcgcgttct ccgtcgttgc caagccagat cccggcaaca ggcgttcccg cggcgacgtc
     3361 gaacaacgcg tcggcatggc cggtgccgcc tcgctcgccg atcgcgagaa tcaggtcggc
     3421 ctcacccgcc agctcgcgtt gcagcggcgt ccgctcgtag agcgagagct gaccggtcga
     3481 gagcggatgc gtgtcgggaa acgccccggc tgcatcaccg gccaggatca ccggcgcttc
     3541 gagcagttcg gccagctcgg cgagttcggt atcggccatc gtcgcgcgga cgccctttcc
     3601 ggcccagatg accggctttg agctgccggc gatcagggaa gtcagcgatg ccaggctggc
     3661 cggatcgagg ctgaccggcg cgatctcggt cggctggtag acatccagct ccgtttcgcc
     3721 ggcagtgatg acgtcctgcg gaatctcgat gtggaccggt cccggccggc cgctggtggc
     3781 gatcgcgaac gcccgcgaga ggacaccggg gatatcttcg acacgctcga ccacgacgct
     3841 ccacttggtc acttcggcaa acatccggac gaggaagtcc ggcttgtcga cgccgtggaa
     3901 cgattcgaag gaggcgttgg ttggcacggc gccgctgatg tggaccatcg gagaggcggc
     3961 catataggcc tgtgcgacgc cagtcagcga gttcgtcgcg ccgggaccgg cagtcaccag
     4021 gacgacgccg ggcttgccgg tcagacgtcc gtaggcgtcg gccatcccgc tggcagtcgt
     4081 ctcgtgcttg acggtgatgt gcttgatctc cggggcgtcc gcaagcgcgt cgtagaccga
     4141 aatgacgtgc gagccgacca ggccaaagac gacctccacc ccctcggccc gcagtgcgtc
     4201 gacgactgcg tccgccgccc gccgtcggac tcgtgttccc tgatgagtca gcatccccaa
     4261 gacctcccaa tatccggttg cacctgtctg aattcgcgca cgactgattg ccggcagagt
     4321 atcgcgtgtc ctgccggaac aggcaaccgg acgtgtggcg atggcgtgat ctgactagag
     4381 ctgatcgatg gtggggtccg aaacggcgtc gtcggccgcc ggctcgctga cgtgatgacg
     4441 cttgcggctg gctgactcgc tcgcgcggcg cttgccaaag cggcccgtga agaaggagat
     4501 cgcgggaaca gcgagaatcg cgatgaccag gacaagcgcc tcatcccacg agaagccact
     4561 ggcatgcaaa atcggcaacg gcagtgacat tcgcgtcact cccttccagg acgttcaaac
     4621 tgcctcccgc gagtataccg ctcggcgctg accgcctgcg gcattcgatc gcctcggcac
     4681 gtaacgtgct ccggtgcgac tgctgccgtc gatattatct gtatgatgtg gcagtcaaac
     4741 tgtagagtag tgccgaccgc gacacgtgga gggagtttca gatggacgac gaacagcgtg
     4801 ggggctcgat gcccggtttc atgattccaa ttgggccgat catcttgatc gcgatcgtgt
     4861 tcatggtcct gcgctcgcgc cgcaagtcca aggatgaacg ggcgttcgcg cgcatcgtca
     4921 acgccatcga cgattccgag ctgccggacc gcgccaagga cctgctgcgc cagagcgttg
     4981 atgaggtgaa gggtgcgatg gcgtcgattc gagagatggc cggcgagctg cgcagctagc
     5041 tgcggtcgat cagcgcctcg acctcggccc gtcgggggac ggacgacagg ccatcgccgg
     5101 aaacgttcag cgcggcggca gccgatgcgt agcgcgccga ggcggagact gactgacgct
     5161 cggagcgggc cgcgaacagc gccgccgcga agacatcgcc ggcgccggtc gtatcgacga
     5221 ccttgatccg gtaggcagga atgtcaactt cccgaccgcg atcgatcagc gacgcgccct
     5281 gcatgccgcg ggtgatggcg ctcaacccac gctcgccgac ctggacgacg atgtcgcgcg
     5341 agtgggtgta ctcctcggcc gagacgacca gcgcgtcgat ccgtgagatg aggtcggccg
     5401 gtagccggag cgggaccgcg cgaaccttgc cgagtcgttc ctcatcccag cggcgcatcc
     5461 agccctgcgg cgtgcagccg agcagtcgcg gcgagaggcg gctgacctgg cgtggctcga
     5521 tctcctgggc gacgggcgcg aggtggatcg cgccggccga gcgccagagc ggtggcaggc
     5581 cggtcaggtc gatctcgccg ccccagtcgt ggatctgctg tgagcgtcgg ccggcgacct
     5641 cggtgttggt gaaggtcgtg atgccgttgc tggcctgagt gacgatctcg acctcggctg
     5701 cgatttcgtc aagctgccgg cgttgctgtc cgtcgagcgc agcgaggttg gcgcgagtca
     5761 ggatggcagc ccgcaagccg tagcgcgcgg ccgtcagggc agcgtagagc actgacccgc
     5821 cgagcatatc tcccgacggt gtccggtcga tggtcatgtg gccgatggcg acgtaatccg
     5881 ggacgcggcc gctgtggctg gcggtcattc gctccatgct ttcaacgccg gtatgggttg
     5941 catgaccaca ccggcgctca ctcggcccgt cagcgacggc taga
//
LOCUS       DASUGJ010000463         3562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1461375, whole genome shotgun sequence.
ACCESSION   DASUGJ010000463 DASUGJ010000000
VERSION     DASUGJ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461375"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1561
                     /gene="pcaB"
                     /locus_tag="VFV93_10260"
     CDS             209..1561
                     /gene="pcaB"
                     /locus_tag="VFV93_10260"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEX5165769.1"
                     /translation="MQQTLFQTPDMVAIFSAEAHVAALLVFEAALARAEARAGVIPAA
                     AADAIAAACRVELFDVAALYRDAVAAGTLAIPLVKALTERVGGEHGRYVHWGATSQDA
                     IDSALMLQMGRGLDLLLDGLLAIGEATAALAEAHRRTPMVGRTLLQQALPITFGLKAA
                     RWLALVSRQLRRLARVQAEGLVVQFGGAVGTLAALGESGLAVTELLAEELGLGVPDLP
                     WHTERDRIAEVVTALGVVAGAMAKIAGDVVLLAQTEVGELAEAMAPGKGGSSAMPHKR
                     NPVDATFAIASARLAIGAIPALLGALEQAHERDTGGWQLEWSLLPDVFGQTAGAVERV
                     RHVVGGLTVDAERMRANLDLTGGLMMAESLTMVLAATIGRPEAFRLVEAATKRARDGN
                     LSLRDAALGDEQIRARLSPEQIDHALDPLAYLGSTDQLIDRALAGFETVRRVHASPSQ
                     "
     gene            complement(1705..3039)
                     /locus_tag="VFV93_10265"
     CDS             complement(1705..3039)
                     /locus_tag="VFV93_10265"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5165770.1"
                     /translation="MTLALGIPVAADLNLVPGEPAHVAYTNGDGVNVRDYPGYSGSII
                     STLNEGVTVTIVDGPMWLDDDSAWFLAGADTYDGYIEGWIVADYLSGEYVEPTYRETT
                     SVVADGTTATVAGTDGYGLRLRDGATTDAGTITVMPEGATVGVLATEIYDASGASWAN
                     VTFDGMTGFAASAYLSIGGGSAPPAQPVADEPDSSGGLAIGDHAAVVGTGGGGLNLRD
                     DASYNGGVVTILPEGVVVTIVDGPAWDGDGNGWYMVDYSSMTGWVHGGFLAWSDQAPS
                     SDPTLDAVAQPVTDAPATEEPVTPEPTPAPTQQPVDPEPVAPETGVGAIVVSEALNFV
                     GTPYVWGGTTPGGFDCSGFTYYIFNNVAGVGLSRSLEVQASSGSYVNPENLQPGDLIF
                     QQNTYKWGLSHVGIYIGGGQMVNAQSERVGVAIADIWDSYWGPRYYTARRVV"
BASE COUNT          591 a         1119 c         1257 g          595 t
ORIGIN      
        1 catgggcggt cgccgaggat tccggcatac gttcctgaac gagccgagcg ccgggtcaac
       61 gaggaacgag atccttcgct ttcgctcagg atgacagggg agaggggtgc ggctccgcaa
      121 caacgccgtt cgtctggctc gcatcagcgg ccccgccccc gagtcacgaa ccccgaatcc
      181 cgaaccccga cccaccagga gaaacgacat gcaacagacg ctctttcaaa cgccggacat
      241 ggttgcgatc ttctcggccg aggcacatgt ggcggcgctg ctggtgttcg aggcggcgct
      301 ggcgcgggct gaggcgcggg ccggggtgat cccggctgcg gcggcggatg cgatcgcggc
      361 ggcttgccgg gtggagctgt tcgacgttgc ggcgctctac cgggacgcgg tggcggccgg
      421 tacactggcg attccgctcg tcaaggcgct gacggagcgc gttggaggcg agcatggccg
      481 ctacgtccac tggggcgcga ccagccagga cgcgatcgat agcgcgttga tgctccagat
      541 ggggcgcggg ctggatctgc tgctggacgg cctgctggcg attggggagg cgacggcggc
      601 gctggctgag gcgcaccggc ggacgccgat ggtcggccgg acgttgctcc agcaggcgct
      661 gccgatcacg ttcgggctga aggcggcccg ctggctggcg ctggtcagcc ggcagctgcg
      721 gcggctcgcg cgcgtgcagg cggaggggct ggtggtccag ttcggcggcg cggttgggac
      781 gctggcggcg ttgggcgagt cggggttggc ggtcactgag ttgctcgccg aagagcttgg
      841 cctcggcgtg ccggatctgc cctggcatac cgagcgggac cggatcgcgg aggtggtgac
      901 ggcactcggc gtcgtggcgg gggcgatggc gaagatcgcc ggcgatgtag tgctgctggc
      961 gcagaccgag gtaggcgagc tggccgaggc gatggcgccg ggtaagggcg gctcgtcggc
     1021 gatgccgcat aagcgcaacc cggttgacgc gaccttcgcg atcgcctcgg cccggctggc
     1081 gatcggcgcc atcccggccc tgctgggcgc gctggagcag gcgcacgagc gcgataccgg
     1141 cggctggcag ctggagtggt cgctgctgcc cgatgtcttc ggccagacag ccggcgctgt
     1201 cgagcgggtg cgccacgttg tcggggggct gacggtcgat gctgagcgga tgcgggccaa
     1261 cctcgacctg accggcgggc tgatgatggc cgaatcgctg acgatggtgc tggctgccac
     1321 gatcggccgg ccggaggcgt tccggcttgt cgaagccgcg acgaagcgtg ccagagacgg
     1381 caacctgtcg ctgcgggatg ccgcgctcgg cgacgagcag atccgggcca ggttgtcgcc
     1441 tgaacagatc gaccatgcgc tcgacccgct ggcctacctc ggcagcaccg accagctgat
     1501 cgaccgggcg ctggccgggt ttgagactgt ccggcgtgtc cacgccagcc ccagccagta
     1561 ggggcgaggg gctgggcggt cagtgcctcg cccctaccgg acggatgttg cctggggtat
     1621 cgtcaacccg gtcgaactaa tgaaggggat ggctcaattg agccatcccc ttcatccccc
     1681 caaccccggt gttgtctgcc ggaactagac gacgcggcgg gcggtgtagt agcgcggtcc
     1741 ccagtagctg tcccagatat cggcgatggc cacgccgacg cgttcgctct gagcgttgac
     1801 catctggccg ccgccgatgt agatgccgac gtgcgagagg ccccacttgt aggtgttctg
     1861 ctggaagatc aggtcacctg gctgaaggtt ttccgggttg acgtacgagc cggaggatgc
     1921 ctgcacctca agcgagcggg agaggccgac cccggcgacg ttgttgaaga tgtagtaggt
     1981 gaagcctgag cagtcgaagc cgcccggtgt cgtgccgccc cagacatacg gggtgccgac
     2041 gaagttgagc gcttcggaga cgacgatcgc gccgacgccg gtctctggtg cgaccggctc
     2101 cggatcgacc ggctgctggg tcggcgccgg tgtcggctcg ggcgtgaccg gctcttcggt
     2161 ggccggcgcg tcagtcacag gctgggcgac ggcgtcgagg gtcgggtcgc tggatggcgc
     2221 ctgatcggac caggcgagga acccgccgtg gacccagccg gtcatgctgg agtaatcgac
     2281 catgtaccag ccgttgccgt cgccatccca ggccgggccg tcaacgatcg tgacaacgac
     2341 accctcgggc agaatcgtga cgacaccgcc gttgtagctg gcatcatcgc gcaggttgag
     2401 gccaccgcca ccggtgccga caacggctgc gtgatcgccg atggcgaggc cgccggacga
     2461 atccggctcg tcggcgacgg gctgcgcggg cggcgcggag ccgccaccga tcgagagata
     2521 ggccgacgcg gcgaagccgg tcatcccatc aaaggtgaca ttggcccagc tggcgccgct
     2581 ggcgtcgtag atctcggtcg cgaggacgcc gacggttgcg ccctccggca tgacggtgat
     2641 cgtgccggca tcggtcgtcg cgccatcgcg caggcgcagg ccgtagccat cggtgccggc
     2701 aacggttgcc gttgtgccgt cggccacgac gctggttgtt tcacggtagg tcggctcgac
     2761 gtattcgccg gacaggtagt cggcgacgat ccagccttcg atgtagccat cgtatgtatc
     2821 ggcaccggcc aggaaccacg cggaatcatc gtcgagccac attgggccat cgacgatggt
     2881 gacggtgacg ccttcattga gggtgctgat aattgagcca ctgtagccgg gatagtcccg
     2941 gacattgaca ccgtcgccgt tggtataggc aacgtgcgct ggttcacctg ggacgagatt
     3001 gagatcggca gcgactggga ttccgagagc tagcgtcatt gtcgccgcga ctactactgc
     3061 cagtagcgtg cgactctggc tcatgcgtct caacgagttt ccctcctacg acaccccgtg
     3121 agggaaccca gttcctgccg aacctacgcg gcagacgcat tgcagtatgt cgcaacaccc
     3181 acaacacgac ggattcaccc cagagcagat acccatcgca atccccaaac gcgacggata
     3241 cggacctgct cgaaaaccct cccgaagctg tccacgaaac gtacctacga cggcattcgg
     3301 ccatgcctgc gataccccta tcgcactgcc gaatgaccag gccacatata cacgtggccg
     3361 ttcggttcac gattgtgccg gcgtataccc cacagtacag ccaccactca tcgtgttgcc
     3421 gccagacggt tacatcgtct cttgggtctg ctggcgtctc cagatcagcc ctcgagacgc
     3481 ctttgtctca ggacgggtgc accttagcac acgcgcaaag atcatgtcaa ccatttccta
     3541 catagtgtac ggacacgaaa ga
//
LOCUS       DASUGJ010000464         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_628219, whole genome shotgun sequence.
ACCESSION   DASUGJ010000464 DASUGJ010000000
VERSION     DASUGJ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628219"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1664
                     /locus_tag="VFV93_10270"
     CDS             <1..1664
                     /locus_tag="VFV93_10270"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX5165771.1"
                     /translation="ILNLARSAGVEIELATDDPLRRELAKILGFRLVADTSPATDRQQ
                     ESNVTGPADSKTFKIVRPAAPETVDVTADEPDWHDAETRPGLTPWRTTESPIPADEPD
                     DADDDIDDGSYSFVIHPPTTRRAVTQPFEQPARQHETVPSFEIWEAGHEPFEAWPERS
                     SSRRLRPFAWISLLLTVALVGAMLVMFLLPAATIAVTPVESQVSASLTYGVALPGTNW
                     DIPIQPVTLSTTLTFSATSPTTGERFEPDASAHGSLMLTNASTGEVLVPAGTIMATET
                     GIQFATVADVTVPAADPYGSLTIGSATVEVTATAPGPDSNVAAETVFGQLDSGIYFLN
                     RDPIGGGTTRRIATVSQADIDTLTARAQQDLNGKAPGAIDRELKDGQQLLANTETRGQ
                     ISTSFNHAAGSDAESLKLDASLTITAQAYSLDGIHDQARDAVADRLRSQAGNEVTVLS
                     QTLKTSTPQPVAGTDATAFSVDASATTRANISSEELDALRAELSGKDDEAAVARIKQL
                     AGVGDVTIDHSPGWLGGRMPRLDSRIQIEVVDAARVQTQTTSSRP"
     gene            1621..2109
                     /gene="ruvX"
                     /locus_tag="VFV93_10275"
     CDS             1621..2109
                     /gene="ruvX"
                     /locus_tag="VFV93_10275"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX5165772.1"
                     /translation="MPREYRRKPRRPGPKLLGLDLGERRIGVAVSDDTGIIASPERIL
                     DLRRASLADVAGLAVTYKVDGIIVGLPKGMSGSEGYQARETRAMAAEIEALVDVPIIF
                     WDERLSSQIADQALSRRVKSPRDRRTQLDAIAAAVTLQSYMDANPIDRSTPGPIEPES
                     RS"
BASE COUNT          401 a          758 c          650 g          302 t
ORIGIN      
        1 agattttgaa cctggcccgc tcggccggcg tcgagattga gctcgcgacc gacgacccgc
       61 tccgccgcga actggcgaag attctcggct tcaggctggt cgccgatacc tcacctgcga
      121 ccgatcgcca gcaggaaagc aatgtaaccg gcccagccga cagcaagacg ttcaagatcg
      181 tgcgcccggc cgctcccgaa acggtcgatg tgacggccga cgagccggac tggcatgacg
      241 ccgaaacccg accgggcttg acgccgtggc gcacgaccga atcacccatt ccggccgacg
      301 aaccagacga tgcagatgac gacatcgacg acggctcgta cagcttcgtc atccacccac
      361 caacaacccg acgggctgtc actcagccgt tcgaacagcc ggctcgccag catgagacgg
      421 tgccctcgtt cgagatctgg gaggccgggc acgaaccgtt cgaggcgtgg ccggagcggt
      481 caagctctcg ccgcttgcgg ccgttcgcct ggatcagcct gctgctgacg gtcgccctcg
      541 ttggggcgat gctggtcatg ttcctgctgc cggccgcgac aatcgccgtc acgccagtcg
      601 aatcgcaggt cagcgccagc ctgacgtacg gcgttgcact gcccggcacc aactgggaca
      661 tcccgattca gccggtcacg ctcagcacga cgctgacgtt cagcgcgacg agcccgacga
      721 ccggagagcg ctttgagccg gacgcatccg cccacggcag cctgatgctc accaacgcat
      781 cgaccggcga ggtgctggtg ccggccggca cgatcatggc gactgagacc ggtatccagt
      841 tcgcgacggt cgccgacgta acagttccgg ccgccgatcc ctacgggtcg ctgacgatcg
      901 gtagcgccac agtcgaggta acggcgactg cgccaggacc cgacagtaat gttgccgccg
      961 agacggtttt cggacagctc gacagcggca tctacttcct caaccgggac ccgatcggtg
     1021 gcggtaccac ccgccggatc gcgaccgtca gccaggccga catcgacacg ctgacggcca
     1081 gggcccagca ggatctcaac ggcaaggcgc ccggcgcgat cgaccgcgag ctcaaggacg
     1141 gccagcaatt gctggccaac accgagactc gcggccagat cagcaccagc ttcaatcatg
     1201 cggcgggctc cgatgccgaa tcactcaagc tggacgcctc gctgacgatc accgcccagg
     1261 cgtacagcct cgacggcatc cacgaccagg ctcgcgacgc ggtcgccgat cggctgcgca
     1321 gccaagccgg caatgaagta acggtgctgt cacagaccct caagacctcg acgccgcagc
     1381 cggttgccgg cacggacgcc accgctttca gcgtcgacgc gagcgcgacg acgcgcgcca
     1441 atatcagctc cgaggaactc gacgcgctgc gcgcggaact ctccggcaag gacgacgaag
     1501 cagccgtggc acgcatcaag cagcttgcag gagttgggga cgtcacgatc gatcactccc
     1561 ccggctggct cggcggacgg atgccgcgcc tggattcccg tattcagatc gaggtcgtcg
     1621 atgccgcgcg agtacagacg caaaccacgt cgtcccggcc ctaagctgct tggcctcgac
     1681 ctcggcgagc ggcggatcgg cgtggccgtc tccgacgaca ccggcatcat cgccagcccg
     1741 gagcgaatcc tcgacctgcg ccgcgcctcg ctggccgacg tcgccgggct ggccgtcacc
     1801 tacaaggtcg acggcatcat cgtcgggctg ccgaaaggga tgagcggctc cgagggctat
     1861 caggctcgcg agacgcgggc gatggcggct gagatcgagg cactggtcga tgtcccgatc
     1921 atcttctggg acgagcgcct ctcaagccag atcgccgacc aggccctctc acgtcgcgtc
     1981 aaatctcccc gcgatcgccg cacccagctc gacgccatcg ccgccgccgt cacgctccag
     2041 agctacatgg acgccaaccc gatcgatcgc tcgacgcccg gacccatcga gcccgaatct
     2101 cgatcctgag a
//
LOCUS       DASUGJ010000465         3612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_72489, whole genome shotgun sequence.
ACCESSION   DASUGJ010000465 DASUGJ010000000
VERSION     DASUGJ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72489"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1183
                     /locus_tag="VFV93_10280"
     CDS             83..1183
                     /locus_tag="VFV93_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5165773.1"
                     /translation="MTIETRLKEFMLSLSFEMDDELLALVGRAGSGKTAVLRAIAGVY
                     VPDSGSIAIHDEPVFSTGLGISVPPAERFVGYVPQAHQLLPHLTVAENVGFLLNRRRD
                     QTPAQQQDAQRRIDELLELLQLTAIADVLPVDLSDLEKQRAALGRALSLDPEVLLLDD
                     PFATLDTGSRRQARSELSELRRLIHVPVLLATTELEEAYEIADRIALVDRGSLLQVAP
                     PRQLLMRPASRRVAEIVRSVNVIPGTIIATPGDAIEVSTPLGILRVNDLSGIHGEVDV
                     VVRPEHVRPLERSETASEDQATNVLHGIITSDVRHGSIHALTFKPDDAYHDVELQVFV
                     SDLAYQQLGLAAEQHRSVELPPRALHLMPRAE"
     gene            1309..2619
                     /locus_tag="VFV93_10285"
     CDS             1309..2619
                     /locus_tag="VFV93_10285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX5165774.1"
                     /translation="MSAGQMTLALSGDSLITRRIAVYEDEPTRRLLEILRAADVAFTN
                     LETVPNNFQGYPAEESGGSHLGAHEWVLDELVSAGFNLFATPNNHSLNYEIPGLLALI
                     DILDRRGLAYAGIGRNLAEARMPAYLDTAAGSVALVACASTFAKGQQASEQRPDSTGR
                     PGLNPLRFKTTYYVTQQQRDTLREITAGLGLEQRRLDAIQLGFRFPPDDPDVLPFLDN
                     NFKVGAPRIETEPNEKDLDANALWVREASMRSDLVVASLHSHEQGATKEDPADFIFTY
                     ARRMIDEGADVVVGHGPHLLRGMEIYKGKPIFYSLGNFVGQNELTFKLPADSYETFRI
                     DPATPPGALYRERSQNDTKGFPTDRRYWQSIVPRCTWRDGALASIELFPVSLALGEAN
                     HRRGRPRLAVGDEATEILTRFGELSNVFGTSIRLDGEQASVVLG"
     gene            2766..3284
                     /locus_tag="VFV93_10290"
     CDS             2766..3284
                     /locus_tag="VFV93_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5165775.1"
                     /translation="MSAGAGNAIHRPVTSSNGTATAAESDEAEPVTTGDAQPATDAGV
                     VESESGRGHVLIVEDDLIIADLIDQILRFESFHVSHVIDGRSAVNYATTHHPDLILMD
                     LMLPVMSGMDASRMIKLHPDCHVSQIPIVAMSAGVNLRATAHGLSVEGVLAKPFDIDE
                     LLATVDIHIRRP"
     gene            complement(3338..3412)
                     /locus_tag="VFV93_10295"
     tRNA            complement(3338..3412)
                     /locus_tag="VFV93_10295"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3378..3380),aa:Met,seq:cat)
     gene            complement(3468..3586)
                     /gene="rrf"
                     /locus_tag="VFV93_10300"
     rRNA            complement(3468..3586)
                     /gene="rrf"
                     /locus_tag="VFV93_10300"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          654 a         1131 c         1160 g          667 t
ORIGIN      
        1 gggagtctag caggcatcgg ctgcacatca cctgcacgcg cggccgtttc cgggtacgct
       61 tctgcctgtg actgcgctaa cggtgacgat cgaaacccgg ttgaaagagt tcatgctgtc
      121 actctcgttc gagatggacg atgagttgct ggcgcttgtc ggtcgcgctg gttcggggaa
      181 gactgccgtc ctgcgcgcga ttgccggtgt ctacgtgccg gacagcggct cgatcgcgat
      241 tcacgacgag ccggtcttct cgaccgggtt gggtatatcc gttccgccag ccgagcgttt
      301 cgtcgggtac gtcccgcagg cacatcagtt gttgccacac cttacagtcg cggagaacgt
      361 tggcttcttg ctgaatcgcc ggcgcgacca gacacccgcc cagcagcagg atgcccagcg
      421 caggatcgat gaactgctgg agctgctcca gttgacggcg atcgccgacg tcctgccggt
      481 tgacttgtcg gacctagaga agcagcgcgc ggcgctgggc cgggcgctct cgctcgatcc
      541 cgaggtcctg ctgctcgacg acccgttcgc gacactggac acgggtagcc ggcgtcaggc
      601 acgctcggaa ctctccgaac tacgccggct catccacgtg ccggttctgc tggcgacgac
      661 cgaactcgag gaggcgtatg agatcgctga tcggatcgcg ctggtcgatc ggggcagctt
      721 gctgcaagtc gccccgccgc gccaactgct gatgcgaccg gccagccggc gtgtggcaga
      781 gatcgtccgt tcggtgaatg tgattccagg gacgatcatc gcgacacccg gcgacgcgat
      841 cgaggtgtcc acgccgctag gcatcctgcg cgtcaacgat ctcagcggga tccacggcga
      901 agtcgatgtc gtcgtccgtc cggaacatgt ccgcccgctg gagcggagcg agacggccag
      961 cgaggaccag gcaaccaacg tgctgcacgg gatcatcact agcgatgtcc gacacggctc
     1021 gatccatgcg ttgacgttta aacccgacga tgcctaccac gatgtcgaac tccaagtctt
     1081 cgtcagcgac ctcgcctacc agcagcttgg cctggcggcc gagcagcatc gttcggtcga
     1141 actaccgccc cgcgcgcttc atctgatgcc ccgcgctgaa tgaatccgga aggaccgata
     1201 tcgccgactg gccgggcgtc gccaggatgt cgacaaggct tccggggttg actggtcgtc
     1261 cacagtcggt agagtaggcc tgccaacggg ggaaaggggg cacagtcgat gtcagcggga
     1321 cagatgacgc tcgcgctgag cggggatagc ctgatcacgc gccggatcgc ggtctacgaa
     1381 gacgagccga ccagacggct gctggagatt ctacgggcag ccgatgtggc attcacgaac
     1441 ctcgaaaccg tgccgaacaa cttccagggc tatccggctg aggagagcgg cggctcgcat
     1501 ctcggcgcgc acgagtgggt gctggacgag cttgtcagcg ccggtttcaa tctcttcgca
     1561 acaccaaaca accatagcct gaattacgag atccccggct tgctggcgct gatcgacatc
     1621 ctcgatcggc gtgggctggc ctatgctggg atcggccgta atttggccga ggcgcggatg
     1681 ccagcctatc tggataccgc cgccggctca gtcgcgctgg tcgcttgcgc ctcgacgttc
     1741 gcgaagggcc aacaggccag cgagcagcgg cccgattcca ccggccggcc ggggcttaat
     1801 ccgctgcgct tcaagacgac ctattatgtt acgcaacagc aacgtgatac cttgcgcgag
     1861 atcacggccg gcctcggcct cgaacagcgc cggctggatg ccatccagct cggcttccgc
     1921 ttcccgcccg acgatccaga tgtactgccg tttctggaca ataacttcaa ggtcggagcg
     1981 cccaggattg agaccgagcc gaacgagaag gatctcgacg ccaatgcgct ctgggtgcgc
     2041 gaagcgtcga tgcgctccga tctggtcgta gccagtctgc actcccacga gcagggcgcg
     2101 accaaagagg acccggcgga tttcatcttc acctacgctc ggcggatgat cgacgaaggc
     2161 gctgatgtcg ttgtcggtca tggcccgcac ctactgcgcg ggatggagat ctacaaggga
     2221 aagccgatct tctacagcct cggcaacttt gtcggccaga acgagctgac gttcaagctg
     2281 ccggcagatt cgtacgagac gttccggatc gacccggcga caccacccgg cgcgctctac
     2341 cgcgagcgca gccagaacga caccaagggc ttcccgaccg accgtcgcta ctggcagtcg
     2401 atcgttcctc gctgtacctg gcgtgatggt gcgttggcgt cgatcgaact gttccctgtc
     2461 tcgctggcgc tgggcgaagc gaaccaccgg cgcgggcggc cccggctggc ggttggtgat
     2521 gaagcgacgg agatcctgac ccgtttcggc gagttgtcga acgtgttcgg aacgtcgatc
     2581 cggttggacg gcgaacaagc atcggttgtg cttggttgaa ccgttcacgg ctgagacggt
     2641 gagtatctac gacgcgtacc gtccgatggc gctgcgcact tcctgcccgg tcgcagcgcc
     2701 gccagccgtt gacatgcgcc tacccgaagg catacggtat gctcggtcag aggaggtagg
     2761 tcgtgatgag cgcaggtgcc ggcaacgcca tacatcgacc cgtcaccagt tccaacggaa
     2821 ccgcaacggc ggccgagtcg gatgaggctg aaccggtaac gactggcgac gcgcagcctg
     2881 cgaccgatgc tggggtggtc gaatccgagt ccggccgggg gcatgtcctg attgttgaag
     2941 acgatctcat cattgctgac ctgatcgatc agatcctgcg cttcgagagc ttccacgtct
     3001 cgcatgtcat cgatggcagg tcggccgtca attacgcaac gactcaccac ccggacctga
     3061 ttctgatgga cctgatgctg ccggtcatga gcgggatgga tgctagccgg atgatcaagt
     3121 tgcatccaga ttgccacgtc agtcagattc cgatcgtcgc catgtcggcc ggcgtcaacc
     3181 tgcgggcgac ggcgcacggc ctgtctgtcg agggcgttct ggcgaagccg ttcgatatcg
     3241 acgagttgct ggcgacggtg gatatccata ttcgccggcc gtgatcggcg tgttgcaggt
     3301 atgccaaagc cccggcacgt cggtgccggg gctgacctgg tagcgggggc gggattcgaa
     3361 cccgcgacct ttgggttatg agcccaacga gctgccactg ctccaccccg cggtggttga
     3421 gggaacatcc ggatcagcca tctcaagcag atatcgcaac tggggagtgt gcgcagcggc
     3481 ctcgcctccc acacggttcc ccgtgcagta ctctccggcg ctgccatggg gcacgaccag
     3541 gttcgggatg gagctgggtg gagccatggc gctctcggcc acgcacacac cccaggtgcg
     3601 ctatctgtct cg
//
LOCUS       DASUGJ010000466        13129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_212996, whole genome shotgun sequence.
ACCESSION   DASUGJ010000466 DASUGJ010000000
VERSION     DASUGJ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13129
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212996"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFV93_10305"
     CDS             <1..552
                     /locus_tag="VFV93_10305"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX5165776.1"
                     /translation="RRLMTDFERASYSTLNMLSNEPQQQATQSLARLTEQVEAPLTHF
                     LHRLNPSVSYVIVPIFAFANAGIPVIDGFSDALGSPVTWGVIAGLLVGKPLGITFFAW
                     LSVRTRIALLPEAIRWRHVFGVAWLGGIGFTMSLFISELAFGADPLADVARIGILIGS
                     IIAGGIGYLLLSATLPRERQPSA"
     gene            627..887
                     /locus_tag="VFV93_10310"
     CDS             627..887
                     /locus_tag="VFV93_10310"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5165777.1"
                     /translation="MDIVIWLIVGAIAGVAAVLVMYRSIPDTPVEWIGAIGVGLVGGW
                     LGGWVGDLIGLEAVNWIGSIVIAFVAAVLLLMLLRRVAPGEH"
     gene            complement(884..1510)
                     /locus_tag="VFV93_10315"
     CDS             complement(884..1510)
                     /locus_tag="VFV93_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX5165778.1"
                     /translation="MLESQETIGTFLLVLDLAGTFVFALSGGMAGVRFRLDLFGVLVV
                     SVVAAMAGGIIRDVLIGALPPAAINDWRYVVVSCVAGLIAFRWHALIAKLQHPVLIFD
                     GAGLALFAVTGTSKALAFQLDPLGAILLGVLTATGGGALRDVLVARPPVVLRSEIYAV
                     TALAGAVVVAGGDRLDLSSRPVALAGAALCFGLRLAAIRRGWELPSAR"
     gene            1679..2452
                     /locus_tag="VFV93_10320"
     CDS             1679..2452
                     /locus_tag="VFV93_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEX5165779.1"
                     /translation="MSDTPAQPQPVPKLPNGIHACLFDLDGVLTQTAKVHAAAWKDMF
                     DAYLRERSARTGEPFVPFDIATDYALYVDGKLRQDGVRSFLASRQIFLPEGTFDDPPT
                     AETVHGLGTRKNDLVQTIIHRDGVETYPGSVRFLEAVHAAGHATAVVSASRNCKAVLE
                     ATGLDAMFDVRIDGVVAAERSLPGKPAPDTFLAAAADLAVDPSEAAVFEDAVAGVAAG
                     RAGNFGWVVGVDRVGHAQALRDNGADVVVADLSELLEEQ"
     gene            2449..4842
                     /locus_tag="VFV93_10325"
     CDS             2449..4842
                     /locus_tag="VFV93_10325"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX5165780.1"
                     /translation="MIEPGVFTVEPWAVTEPELRLDLMGQTESIFALSNGHIGFRGNF
                     DEGEPHHTPGTYLNGFFETLPMPYAEGGYGYPEEGQTLIDVANGKLLRLLVDDEPFDI
                     RLGTLVEHQRTLDLRDGVLRREVHWISPAGQAVKIRSTRLVSFVQRAVAAIYYEVEAV
                     DGPARIVLQSSLVANEPIPEQLNDPRAAVALRAPLMSEYHTHYDLAASMGHQTRVSGL
                     RMAAGLDHLVDGPPGTVFEAESEPDLARVTVSTELAPGQKLKVVKFIAYGWSSVRSMP
                     ALRDQVDAALAAAKRVGWDGLRAGQREYLDDVWDRADIIVEGDEQLQQAVRFALFQVI
                     QAGARAERRAIPAKGLTGRGYDGHSFWDSETYTLPVLTYTSPDAARDALLWRHSTLDL
                     ARARARELRLGGATFPWRTIRGEECSGYWPAGTASFHINADIADAVRRYKNATGDTEF
                     ERGPGFELLVETARLWLSLGHHDAEGCFRIDGVTGPDEYTALVDNNVFTNLMAARNLR
                     AAADVAGKYPERAAEMGVKQREIDAWLTAADKMVVPFDDELGVTAQSEGFTRYREWDF
                     ENTEEQDYPLLLHYPYYLLYSSQVIKQADLVFALYLFGDAIKPEQKLRDFEYYESITV
                     RDSTLSASIQAIVAAEVGHLHLAYDYFRETAFVDLWDLAGNTDDGVHLASLSGAWLVA
                     VAGFGGMRDHAETLAFAPRLTEPLTRLAFRLLFRGRRIHVDINSDHARYELLAGDPLE
                     IVHHGQPFTLAMDAPQTLPYPALEAPPPITPLPGREACRHGVGANGRLNRKAARHRY"
     gene            complement(5236..6435)
                     /locus_tag="VFV93_10330"
     CDS             complement(5236..6435)
                     /locus_tag="VFV93_10330"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5165781.1"
                     /translation="MSICLAAGDLSRSLPSFAGDWDELFGEVCRNGLLGLTNRYLAVG
                     TSASMVPDTFRAAVEQGCVVSQIHMRAMYYWIFEILRALSDADIEVVVLKGPALAHSI
                     YPSPGLRVFGDLDLMVRERDMLTAHAVLTDHGLLARQMLDKVPPKLTHACTTYELVYR
                     DPASKFVLEMHYDDLLNAGLVSRDLDGYWNRSVLVTLPGAVVRTLSLEDHLLHLCAHT
                     HYHGYVKLNWFSDIAFIVRDRHDELDWDQFVRVTRREEAKVAVFYSLTLLEKLFGIAA
                     PRDVIMAITPDRFRQWAHTYFVPMDRVLGPESMPRPDFSFYHLPVLKRLLPDLLVMGR
                     RREKVRYLLRLLAPPPAWLRSYYQLGATQPIAGHYILHPLKLGWHLGTEIAAIPRRNG
                     PWWHSHA"
     gene            complement(6524..7408)
                     /locus_tag="VFV93_10335"
     CDS             complement(6524..7408)
                     /locus_tag="VFV93_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165782.1"
                     /translation="MLDSQWPRVVPHSPGGEVPDAVVRLLSFSARLFGVQDDVVDALA
                     RIYPHTSRACATRSAFIHADSHIADIRLDDVIGPDLVGVSQISETLGTLEFLLDTLAL
                     RSFDNHLVLHSGAVAVGDMGFLIPAASGAGKSTLVAALAQSGFVVLSDEFAILDIGTL
                     RLRPFPKALCLKTGGRLVVERAFGVSLDTVPVRRVNHQCVSFVSAQRMFPVERCATPG
                     AIVILNRVSSGKSSITAISRAEALATLLQQSLNVPQHGVSGIDTLVGLVESTECLRLT
                     YADARDAVGSIERFVSAI"
     gene            complement(8686..11466)
                     /locus_tag="VFV93_10340"
     CDS             complement(8686..11466)
                     /locus_tag="VFV93_10340"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX5165783.1"
                     /translation="MQEPWRQDVAAVADALGTDIQQGLSSEQAAARLSCYGPNQLEAA
                     AVVPAWRKFLAQFADPLIYLLLGAVAVSFLAWLLEGAEGVPFEVVVILTIILLNAILG
                     YVQEARAEQAVAALQRMAAATAAVLRGGREERIAAVDVVPGDVLLLAEGDAVSADARL
                     VEAASLTVAEASLTGESEAVLKDTATIDAPAGIGDRLNMVFNGTAITRGRGRAIVTAT
                     GMRTEMGTIARLLDTEESKTPLQREVDRIGKMLGIAVIIIAVVVIGAILLTEDIEKAS
                     DYVDVLLVGVSLAVAAVPEGLPAVLSVVLALGVQRMAERNAIVKRLSSVETLGSASVV
                     CSDKTGTLTRNEMTIEKIVTASGEVDVTGSGYRPAGELRAGGDPLDDPVLLDEVRIVL
                     TGGSLSNDAGLREEDGEWLIQGDPTEAAFLVAEGKIEGLAEARQARFTRVGEVPFTSE
                     RKLMSTVQHDADGELGTAVVTKGAPDVLLARCNAERVAGDVRRLAEGRRQEILATVDR
                     LADLALRTLAVAYRPLPGDERPPEDESIEQDLVYLGMVGIIDPPRDEARDAIAEANAA
                     GIRVIMITGDHPRTAARIASDLGIAGTDGRAFTGHQLEQMDAEALRLAVRDVSVYARV
                     APEHKLRIVDALQANENIVAMTGDGVNDAPALKSADIGVAMGITGTDVSKEAADMILA
                     DDNFATIVTAVREGRAIFANIRKFLRFLLSSNIGEVLTMFFGVILAGALGLDKTGEAV
                     AVPLLATQILWINLLTDSAPALAMGVDPPPDGVMGHPPRRLTDRVIDAEMWVSIGFIG
                     VVMAAVTLAALDLRLDGGLLGGSGEIDEARTMAFTTLVFAQLFNCFNSRSDRTSAFNH
                     LFTNAWLWGAIAVSVALQVAVVQLRFLNDAFGTTPLPAGDWLLCAGLASIVLWADEAK
                     KIVENWLRPGPQ"
     gene            complement(11729..>13129)
                     /locus_tag="VFV93_10345"
     CDS             complement(11729..>13129)
                     /locus_tag="VFV93_10345"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5165784.1"
                     /translation="TNGHSLSKVTQGVTTEILGEGWTPAPFGGRIGEGPSAFAEIPEE
                     IVERVKTWLRFSDWVDYMGEVGISVNYGSFLGGGTLREYVKGWELGQQTPEEMETMRR
                     VVREVMEDGAFGVATALIYPPNSYTTDEELYETCRVIAEYGGVYITHMRSEADEFLEE
                     LAETIDLSRQTGCALELYHLKVGGKRNWAKMATAIEMINAARAEGLDITADMYPYIAG
                     GTGLASAIPNWAAADGKLLDNLRDPEVRERIRHELVEPTGGWEPLATLAGPEGVLVTE
                     LKSAGNQQYSGKRLSEIAEARGQHWTDCLMDLVLAEEEWVFTIYFMMNEDNLPMQLRQ
                     PWMKISTDAAGLDPATSGHAVTHPRSYGTYPRVLGKYTREDGVLTLEDAVRKMSSAVA
                     DRLSLRERGQLRAGMYADVVIFNPDTVIDNATFEDPHQLSTGIRDVWVNGVRVLSGGS
                     HTGATPGMPVYGPGRR"
BASE COUNT         2319 a         4410 c         4164 g         2236 t
ORIGIN      
        1 cggcggctga tgaccgactt cgagcgcgcc tcgtattcga cgctcaacat gctctccaac
       61 gagccacagc agcaggcgac ccagtcgctg gcgcggctga ccgagcaggt ggaagcgccg
      121 ttgactcact tcctgcaccg cttgaaccca tcggtctcgt acgtgatcgt gccgatcttc
      181 gccttcgcga atgccggcat tccagtgatc gatgggttct ccgacgcgct cggcagcccc
      241 gtcacctggg gtgtgatcgc cgggttgctg gtcggaaagc cgttgggcat cacgttcttc
      301 gcctggctgt cggtccggac gcggatcgcg ctgttgcccg aagcgatccg ctggcgacac
      361 gtcttcgggg tcgcctggct gggtggtatc ggcttcacga tgtcgctgtt catctcggaa
      421 ctggccttcg gcgccgatcc gctggcggat gtcgcgcgaa tcggcatcct gattggctcg
      481 atcatcgccg gtggcatcgg ctacctgctg ctgtcggcaa cgctgccgcg cgaacgccag
      541 ccgagcgcgt agcggttctg gaatatagat tgcaatagcg tccacgaatg aaccggaccg
      601 atcggttcgc gaaaggagcg ggttccgtgg atatcgtcat ctggttgatc gtcggggcga
      661 tcgccggcgt cgcggccgtc ctcgtcatgt accggtcgat tccggataca ccggttgagt
      721 ggattggcgc aatcggggtc gggctggttg gcggctggct gggtggctgg gtcggggatc
      781 tgatcgggct cgaagccgtc aactggatcg gctcgatcgt gatcgccttc gtcgcggcgg
      841 ttctgctgtt gatgctgttg cggcgcgtcg cgccgggcga gcactagcgg gcggaaggca
      901 gttcccaacc gcgccggatg gcagccagcc gcagtccgaa gcaaagcgcc gcaccggcca
      961 gcgcaaccgg cctggatgac agatccagcc ggtcgccacc ggccacgacg accgcgccgg
     1021 ccaaggcagt gacggcgtag atctcagagc gcaggacgac cggaggtctg gcgaccagga
     1081 catcgcgcag cgcgccaccg ccggtcgccg tcagcacgcc gagcaggatc gcgccgagcg
     1141 ggtccagctg gaacgcgagc gccttcgagg tgccggtcac ggcgaagagg gccagcccgg
     1201 cgccatcgaa gatcaggacc gggtgttgca gcttcgcgat cagcgcatgc cagcggaagg
     1261 cgatcagccc ggcgacgcag gagacgacga cgtagcgcca gtcgttgatc gcggccggcg
     1321 ggagcgctcc gatcagcaca tcgcggatga tcccgccggc catcgccgcc acgactgaga
     1381 cgaccagcac accgaacaga tccagccgga agcgaacgcc ggccatcccg ccgctgagcg
     1441 cgaagacgaa cgtcccggcc aggtccagca ccagcaggaa cgtaccgatc gtctcctggc
     1501 tttcaagcac gcatccccca accgccgctc acgccgatgc catccgtcgg atgatacgtg
     1561 atgcggccgg cctgacccgg ccgggcaagc cgcgtgcgat agcggcagca gaggccagcc
     1621 agcgatcggc cttgctacaa tccggtacag gatcgaccgc cacgacggaa ggccctcgat
     1681 gtcagacacg ccagcacagc cccagcccgt tccgaagctg cccaacggta tccacgcctg
     1741 cctgttcgat ctcgatggcg tcctgaccca gacggccaag gtccacgcag ccgcctggaa
     1801 ggacatgttc gacgcctacc tgcgcgagcg gtcggcgcgg accggcgagc cgttcgtgcc
     1861 cttcgacatc gccaccgact acgcgctcta tgtcgacggc aagcttcgtc aggacggtgt
     1921 tcgctcattc ctggcttcgc gccagatttt cctgccggag ggaaccttcg acgatccgcc
     1981 gacggccgag accgtccacg gcctcggaac gcgcaagaac gacctggtcc agaccatcat
     2041 ccaccgcgac ggcgtcgaga cctacccagg gtcagtccgt ttcctggagg ccgtccacgc
     2101 agccgggcat gcgacagccg tcgtctccgc cagccggaac tgcaaggcgg tcctggaggc
     2161 gaccggtctc gacgcgatgt tcgacgtgcg gatcgacggt gtcgtcgccg ccgagcgcag
     2221 cctgcccggc aagccggcgc ccgacacgtt cctggcggct gccgccgatc tggctgtcga
     2281 tccgtcggag gccgccgtct tcgaggatgc cgtcgccggg gtcgcggccg gacgggccgg
     2341 gaacttcggt tgggtcgtcg gtgttgaccg ggtcggccac gcgcaggccc tgcgtgacaa
     2401 cggggccgat gtcgtcgtcg ccgatctgag cgagttgctg gaggagcaat gatcgaaccg
     2461 ggcgtcttta cggtcgagcc gtgggccgtg acggaaccgg aactacggct cgacctgatg
     2521 ggacagacag agtccatctt cgccctctcg aacggacaca tcggatttcg cggcaacttc
     2581 gacgaaggcg agccgcacca cacccccggc acctacctca atggattctt cgagacgctc
     2641 cccatgccct acgccgaggg tggctacggc tatcccgaag aggggcagac gctgatcgac
     2701 gtcgccaacg gcaagctgtt gcggctgctg gtggacgacg agccattcga tatccgcctc
     2761 gggacgctcg tcgagcacca gcgcacgctc gatctgcgcg acggcgtcct gcggcgcgag
     2821 gtccactgga tctcaccggc cggccaggcg gtcaagattc gttcgacccg gctcgtctcg
     2881 ttcgtccagc gtgccgtcgc cgcgatttac tacgaagtcg aggccgttga cggcccggcc
     2941 cggattgttc tgcaatcgtc actggtcgcg aacgagccga tccccgagca actcaacgac
     3001 ccacgggcgg ccgtcgccct gcgcgcgccg ctcatgagcg agtaccacac gcactacgat
     3061 ctggccgcgt cgatggggca ccagacgcgc gtcagcggtc tgcggatggc ggccgggctc
     3121 gaccacctgg tcgacggccc cccgggaacg gtcttcgagg ccgagagcga gccggatctg
     3181 gcccgcgtca cggtcagcac cgagctggcg ccggggcaga agctcaaggt ggtcaagttc
     3241 attgcatacg gctggtcaag cgttcgctcg atgccggccc tgcgcgatca ggtcgatgcg
     3301 gcgctggccg ccgccaagcg ggtcggctgg gacgggctgc gggccggcca gcgcgaatac
     3361 ctggatgatg tctgggaccg tgccgacatc atcgtcgagg gcgacgagca gctccagcag
     3421 gccgtccgct tcgcgctttt ccaggtcatc caggccggcg cgcgggccga gcggcgcgcg
     3481 atcccggcca aggggctgac cggccgcggc tacgacggcc attcgttctg ggactccgag
     3541 acctacacgc tgccggtcct cacctatacc tcgccggacg ccgcccgcga cgcgctgctc
     3601 tggcggcatt cgacgctcga cctggcccgc gcccgggcgc gggagctgcg cctgggcggc
     3661 gcgaccttcc cgtggcggac gatccgcggc gaggagtgct ccggctactg gccggccggg
     3721 acggcctcgt tccacatcaa cgccgacatc gccgacgccg tccgccgcta caagaacgcg
     3781 accggcgaca ccgagttcga gcgcggcccc ggcttcgagc tgctggtcga gacggcccgc
     3841 ctctggctgt cgctcggcca ccatgacgcg gaaggctgct tccggatcga cggcgtgacc
     3901 ggcccggacg agtacacggc cctggtcgac aacaacgtct tcaccaacct gatggctgcc
     3961 cgcaacctgc gcgctgctgc cgatgtcgcc ggcaagtatc ccgagcgagc ggcggagatg
     4021 ggcgtcaagc agcgcgagat cgacgcctgg ctgacggccg ccgacaagat ggtcgtgccg
     4081 ttcgacgacg agctgggtgt cacggcccag tcggaagggt tcacccgcta ccgcgagtgg
     4141 gacttcgaaa acaccgagga gcaggattac ccgctcctgc tgcactaccc gtattacctg
     4201 ctctattcca gccaggtcat caagcaggcc gatctcgtct tcgcgctgta cctgttcggc
     4261 gatgcaatca agccggaaca gaagctgcgc gacttcgaat attacgagag catcaccgtc
     4321 cgcgattcga cgctgtcggc ctcgattcag gcgatcgtcg cggccgaggt cggccatcta
     4381 cacctcgcct acgattactt ccgcgagaca gccttcgtcg atctctggga tctggccggc
     4441 aacacggacg acggcgtcca cctcgcctcg ctctccggcg catggctggt cgctgtcgcc
     4501 ggcttcggcg gcatgcgcga ccatgccgag acgctggcgt tcgccccccg cttgaccgag
     4561 ccgctgacca ggctggcgtt ccggctgctg ttccgtggcc gccgcatcca cgtcgacatc
     4621 aactccgacc acgcccgcta cgagttgctg gccggtgacc cgctcgaaat cgtccaccac
     4681 ggccaaccgt tcacgctcgc gatggacgcg ccgcagaccc tcccgtatcc ggcgctggaa
     4741 gcgcctccac cgatcacgcc gctccccggc cgcgaagcct gccgccacgg cgtcggcgcc
     4801 aacggccgcc tcaaccggaa ggccgcccga caccggtatt agcctcctgt cgtccttcgc
     4861 tttggctcag cactcgaccg gactggctgt gtcaagagga acacgcccct tgccctgtca
     4921 tcctgagccg cagcgaagga tcgcttggcc ctgacgacga acgtggcgac ccgggatgcg
     4981 aacgttcacc tgccgaatca ccttctcctg tccccaccgc caggcgatcc ttcgctgcgg
     5041 ctcaggatga caggctatgg cgtggagcct cgtcaacgcg ggaagtctaa agcagggacc
     5101 tcttgctcga tcacatcagg cagtgagacg ctggtgcgtc ccgaactggt tcccatatcg
     5161 ggatgcgtgt tgcgctgggc gactgcgcga cggggggccg tctcgctcaa acgccgtgct
     5221 gtgtcatcag atcagctagg catggctgtg ccaccacggg ccatttcgcc ggggaatcgc
     5281 ggcgatctcg gtgcccagat gccagcccag tttcagcgga tgaaggatgt agtgtcccgc
     5341 aatcggctgc gtcgcaccaa gttgatagta gcttcgaagc caggccggtg gcggggccag
     5401 cagccgcagc agatagcgaa ccttctctcg gcgtcggccc atcacgagca ggtccggtag
     5461 tagtcgcttg agcaccggta agtgatagaa gctgaagtcg ggccgtggca tcgactccgg
     5521 tccgagcacc cgatccattg gtacgaagta ggtatgagcc cactggcgaa agcgatccgg
     5581 cgtgattgcc atgatgacat cccgcggagc ggcgataccg aacaatttct ccaatagcgt
     5641 caggctgtag aagacagcga ccttagcctc ctcacgccgc gtgacgcgca cgaactgatc
     5701 ccaatcgagt tcgtcgtgtc gatcccggac gatgaaagcg atatcggaga accagttcaa
     5761 cttcacatag ccgtgatagt gggtgtgagc acagaggtgc agcaggtgat cctcaagcga
     5821 tagcgttctg acaaccgctc ccggtagcgt cacgagcacc gaccggttcc agtagccgtc
     5881 caagtcgcgt gacaccagtc cggcgttgag caggtcatcg tagtgcatct ccagcacgaa
     5941 ttttgaggcg gggtctcgat acacgagttc gtacgtcgta caggcatggg tcagtttggg
     6001 ggggacctta tcgagcattt gtcgagcgag tagcccatga tcggtcaaca cggcgtgagc
     6061 cgtgagcatg tctcgctcgc ggaccatcaa gtcaaggtcg ccgaagacac gcaggccggg
     6121 cgagggatag attgagtggg ccaacgctgg gcccttgagt acgacaacct caatatcggc
     6181 atcggagagt gctcgaagaa tctcgaagat ccagtagtac atcgctcgca tgtggatttg
     6241 tgagacgacg catccctgct cgactgctgc gcggaaggtg tcgggcacca tgctggccga
     6301 cgtgccaacc gcgaggtagc ggtttgtcag accgagcaac ccgttgcggc agacttcgcc
     6361 gaatagttcg tcccaatcac cggcgaaact gggtaacgac cggctgagat cgccagctgc
     6421 caggcagatt gacatctcgc gcgtgctggc cgagaccgac ctccggtcga agccgatctg
     6481 accccaactg cgtgtcaatc gttggtccac aaacctcgtt ctcctagatc gccgagacga
     6541 acctctcgat gctgcccacc gcgtcgcgcg cgtccgcgta ggtgagccgc aagcactccg
     6601 tgctctccac gagaccaacg agtgtatcga ttccagaaac accatgttgc gggacattga
     6661 gcgactgttg gagcagagtg gccaatgctt ccgcacgtga gatagctgtg atcgaggact
     6721 tcccgctgga aacccggttc aggatcacga tcgcaccagg tgtcgcacaa cgctcgactg
     6781 ggaacatgcg ctgcgctgac acgaacgaca cgcactgatg gttgactctt cgaacaggaa
     6841 ccgtatccag cgacaccccg aacgcgcgct cgaccaccaa tctgccaccg gtcttgagac
     6901 aaagcgcttt cgggaacgga cgcaggcgca gggttcctat gtctaagatc gcaaactcgt
     6961 cagacaagac gacaaagccc gactgggcga gcgccgcgac cagtgtcgac tttccggcgc
     7021 ccgaagcagc aggaatgagg aatcccatgt ctccgactgc gacagcaccg gaatgcagca
     7081 cgaggtgatt gtcgaatgac ctgagagcta gcgtgtccag tagaaactcg agcgtgccca
     7141 acgtctcgga tatctgcgac acgccaacca gatctgggcc aatgacgtca tcaagccgga
     7201 tgtctgcaat gtgcgaatct gcatggatga acgcggatcg tgttgcgcag gcgcggctag
     7261 tgtgaggata gatcctcgcc agggcatcaa cgacatcatc ttgaacgccg aacagtcgag
     7321 cggagaagga gagaagtcga acgaccgcgt cagggacctc gccgccaggg ctatgcggaa
     7381 ccacccgcgg ccactgcgaa tcaagcatcc caggctggcc cattcatgtg ctcaggaggt
     7441 tctcattggc gaacgcgagc agcatctctg cgacatctcg ctcgacgaga tcccggctcg
     7501 ctggaaacct ctggacaacc tgttcggcga tgtccgcaat cccaagatct cctgtgcaat
     7561 gcagccagat gacggaagcc gtcgggttca ggacatgtgc gcgctggtgt ctcagatcgt
     7621 agaggacgat gctgtcgtcg acggaccgtt cgacaatctg atcgacgcgc cgccgtggtt
     7681 gggttgcaga gtgaacgtgc atgtcaaccg acgcctcatg gatcgtcact ggcgttcccc
     7741 ttgttcacat cacatcaatt gtcgcggcta attcgtatct ttgggctttg gcttgggctt
     7801 gggcttgggc ttgggctgct tgggatgttt tggcagttta ggttcgtccg acgggccgga
     7861 gctaccacca tcacccccgg tgacggacgg gtgtggcacg gcggctagcc caacgctctg
     7921 catgctcggc gtggcgtaga tgccgctcaa catcacgccg cccgtcaacg caactccggt
     7981 cttcacaaac tcgcgccgtg tgctcctgcc cttgccctga ctgctcgtgc ctgagttgga
     8041 aggctgaacg tcgtctatca tggtcccacc ccttctaacg acgttatcgt acggccacat
     8101 gagccaaaca cttggcggca acgccatcgc agacgatgac aaggttgtca tacagagatg
     8161 cccttggtta tcgctgttcc atgcagtcag aactgcaccg ctgggaggaa cgccatttca
     8221 tagcggctcc ttccctcggt gatcagatcc accaggatct cgccgatcgc gcagcagtct
     8281 cgaacgagca caccgataga ccgactggtg tcgctgcgac gggacgtgct gggatatgag
     8341 atcccctcgc acacgtgctc acgtgcggaa cgctggctaa cacaacgagt gtcgcagact
     8401 atcagtttga acagactgct gaagcagcgg aagcagagga caccgccgac gagccgcaag
     8461 gtgaagatcg tgccaatgtc tccgaatgcg accttgcgta cccctacttc tgcatccccc
     8521 ggtctggaac ggtagagcgg tgtgcagaag gatggtcccg cgatcgtagg gaccggtctc
     8581 agaccgatct gcggcccacc gatcgacacg gtgagacggt ccggcagcaa gggacaacgt
     8641 ttctgcacac cactcttgtt cagacaggcg gagggcgata ttccgttact gcggccccgg
     8701 ccgcagccag ttctcgacga tcttcttggc ctcgtcggcc cagaggacga tgctggccag
     8761 gccggcgcag agcagccagt cgccagcggg gagcggcgtc gtgccgaagg cgtcgttgag
     8821 gaagcgtagc tggacgacgg cgacctgcaa cgcaaccgag acagcgatcg caccccacag
     8881 ccaggcgttg gtgaacaggt ggttgaaggc actggtgcgg tccgagcggc tgttgaagca
     8941 gttgaacagt tgcgcgaaga cgagcgtcgt gaacgccatc gtccgcgcct cgtcgatctc
     9001 gcccgacccg cccagcagcc cgccatccag ccgcaggtcg agcgccgcca gcgtcacggc
     9061 cgccatgacg acgccgatga agccgatgct gacccacatc tcggcgtcga tgacgcggtc
     9121 ggtcagccgg cgcggcgggt gccccatcac tccgtccggt ggcgggtcga cgcccatcgc
     9181 cagcgccggc gcgctatccg tcagcagatt gatccacaga atctgggtcg ccagcagcgg
     9241 gacggcgacc gcctcgccgg tcttgtccag gccaagcgcg ccggccagga taacgccgaa
     9301 gaacatcgtc agcacctcgc cgatgttcga cgagagcagg aagcgcagga acttgcggat
     9361 gttggcgaag atcgcgcggc cctcgcggac ggccgtcacg atcgtcgcga agttgtcgtc
     9421 ggcgaggatc atgtcggccg cttccttgga gacgtccgtg ccggtgatcc ccatcgcaac
     9481 gccgatatca gccgatttca gggccggagc gtcgttgacc ccgtctcccg tcatcgcgac
     9541 gatgttctcg ttcgcctgga gcgcgtcgac gatccgcagc ttgtgctccg gcgcgacccg
     9601 cgcgtagacc gagacgtcgc gaaccgccag ccggagcgcc tcggcgtcca tctgctccag
     9661 ctgatggccg gtaaacgccc gcccgtcggt accggcaatc cccaggtcgc tggcgatccg
     9721 cgccgccgtc cggggatggt cgccggtgat catgatgacc cgaatcccgg ccgcgttcgc
     9781 ctcggcgatg gcgtcgcgcg cctcatcccg tggcgggtcg atgatgccga ccatcccgag
     9841 gtagaccaga tcctgctcga tcgactcatc ctccggcggg cgctcgtcgc cgggcaaggg
     9901 ccggtaggcg acggccagcg tccgaagcgc gaggtcggcc agccggtcca ctgtcgcgag
     9961 gatctcctgc cgccggcctt ccgccagccg gcgcacgtcg ccggccaccc gttcggcgtt
    10021 gcagcgcgcc agcaggacgt ccggcgcgcc cttcgtgacg acggccgttc ccagctcacc
    10081 atccgcatca tgctggacag tgctcatcag cttgcgctcg gaggtgaacg gcacctcgcc
    10141 aacccgcgtg aagcgtgcct ggcgtgcctc agccagcccc tcgatcttcc cctcggcaac
    10201 cagaaaagcc gcctcggtcg gatcgccctg gatcagccac tcgccgtctt cctcgcgcaa
    10261 tccggcatcg ttcgacaggc tgccgccggt cagcacgata cgcacctcat ccagcagcac
    10321 gggatcgtcg agcgggtcgc caccggcgcg caactcgcca gccggccggt agccggagcc
    10381 ggtcacgtcg acctcgcccg aggcagtcac gatcttctcg atcgtcatct cgttgcgcgt
    10441 cagcgtaccg gtcttgtccg agcagacgac cgacgccgag ccgagcgtct cgaccgacga
    10501 gagcctcttc acgatcgcat tgcgttcggc catccgctgg acaccgagcg cgaggacgac
    10561 cgagaggacg gccggcagcc cctctggaac ggccgcgacc gccagcgaga cgccgaccag
    10621 cagcacatcg acgtaatcag acgccttctc gatatcctcc gtcagcagga tcgccccaat
    10681 gacgacgacc gcgatgatga tgacggcgat accgagcatc tttccgatcc ggtcgacctc
    10741 gcgctggagc ggtgtcttcg actcttcggt gtcgagcagg cgggcgatcg tgcccatctc
    10801 ggtccgcatg ccggtcgctg tcacgatcgc ccggccccgg ccacgggtga tggccgtccc
    10861 attgaagacc atgttcagcc ggtctccgat gccggccgga gcgtcgatcg tcgccgtatc
    10921 cttgaggacg gcctcgctct cgccggtcaa tgacgcttcg gcgaccgtca gcgatgcggc
    10981 ctcgaccaac cgggcatcgg cgctgacggc gtcgccctcc gccagcaaca gcacgtcgcc
    11041 cggcacgaca tcgaccgcgg cgatccgttc ctcgcggcca ccgcgcagca ccgccgccgt
    11101 cgcagctgcc atccgttgca gggcagcgac ggcctgctcg gcccgtgctt cctgcacgta
    11161 gccgagaatc gcgttgagga gaatgatcgt caggatgacg acgacctcga acggcacccc
    11221 ttcggcgcct tcgagcagcc aggcgagaaa cgagacagcg acggcaccca gcagcaggta
    11281 gatcagcggg tcggcgaact gcgcgaggaa cttgcgccag gccggcacga ccgccgccgc
    11341 ctcaagctgg ttcggcccgt agcacgaaag ccgggcggcc gcctgctcgg agctcagtcc
    11401 ctgctggatg tcggtcccga gggcgtcggc gacggcggcc acgtcctgac gccagggttc
    11461 ttgcacgatc ggttcgtcca ttcgcgctcc gcacgtccgt cagcctgatc ttggccgcca
    11521 ctgctgctga tccgaacaga tcggagctcg caatggccat gccgtctggc gctgcatatg
    11581 acacgtccca atcaatcccc aatccggtac gggcgatatc gcaatatcgc cctttcaaat
    11641 gcccgacgtc tcgagaaggg cgatattgcg atatcgcccg taccggattg atccattgcg
    11701 attggggctt gtcatgagcg aaacagcatt accttcgccc cgggccatag actggcatgc
    11761 cgggtgtcgc gcccgtgtga ctgccgccgg agaggacgcg aacgccattg acccagacat
    11821 cgcggatgcc ggttgagagc tggtgcgggt cctcgaacgt cgcgttgtcg atgacggtat
    11881 cggggttgaa gatgacgaca tcggcgtaca tccccgcgcg caactggccg cgttcgcgca
    11941 ggctgaggcg gtcagcgacg gccgaggaca tcttgcgaac ggcatcctcc agcgtcagga
    12001 cgccatcctc gcgggtgtac ttgcccagca cgcgggggta cgtgccgtac gagcgcgggt
    12061 gggtgacggc gtgaccggag gtggccggat cgagcccggc cgcgtcggtg ctgatcttca
    12121 tccacggctg ccggagctgc atcggcaggt tgtcctcgtt catcatgaag tagatcgtga
    12181 agacccactc ctcctcggcg aggacgaggt ccatcaggca gtcggtccag tgctggccgc
    12241 gcgcctcggc gatctccgag agccgcttgc cggagtactg ctggttgccc gcgctcttca
    12301 gctcggtgac gaggacgccc tccgggccgg ccagcgtcgc cagcggctcc cagccgccgg
    12361 tcggctcgac cagctcgtgg cgaatgcgct cgcgtacctc cgggtcacgc aggttgtcga
    12421 gcagcttgcc gtccgccgcc gcccagttgg ggatcgccga ggcgagaccc gtcccgccgg
    12481 cgatgtaggg gtacatatcg gccgtgatat ccagcccctc ggcgcgggcc gcgttgatca
    12541 tctcgatcgc agtcgccatc ttggcccagt tgcgcttgcc gccgaccttg aggtggtaca
    12601 gctccagtgc gcagcccgtc tgccgcgaga ggtcgatcgt ctcggccagc tcttcgagga
    12661 actcgtccgc ctccgagcgc atgtgggtga tgtagacgcc accgtactca gcgatcactc
    12721 ggcaggtctc gtagagttcc tcgtcggtcg tgtaggagtt cggcggatag atcagcgccg
    12781 tcgcgacgcc gaaggcgccg tcctccatca cctcgcggac gacgcggcgc atcgtctcca
    12841 tctcctcggg cgtctgctga cccagttccc aacctttgac gtactcccgc agggtgccgc
    12901 caccgaggaa cgagccgtag ttgaccgaga tgccgacctc gcccatgtag tcgacccagt
    12961 cgctgaagcg caaccaggtt ttgacccgct cgacgatctc ctccggaatc tccgcgaagg
    13021 cgctcggccc ttctccgatc cgtccgccga acggggccgg cgtccagccc tcgccgagga
    13081 tctcggtcgt gacgccctgc gtcaccttcg agagcgagtg accgttggt
//
LOCUS       DASUGJ010000467         4546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1046206, whole genome shotgun sequence.
ACCESSION   DASUGJ010000467 DASUGJ010000000
VERSION     DASUGJ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046206"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /gene="panB"
                     /locus_tag="VFV93_10350"
     CDS             complement(<1..570)
                     /gene="panB"
                     /locus_tag="VFV93_10350"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5165785.1"
                     /translation="MSTTPPTREKITAPAILEMKASGEKIVMVTAYDYPTARAVEAAG
                     IESILVGDSLGMVVLGYESTIPVTMEDIIHHTRAVMRGVTSVHVVADLPFMSYQVSDA
                     QALENAGRLMKEGGADAVKLEGGQSMASRIEAIVRAGIPVMAHIGLTPQASAALGGFK
                     VQGRSLEAARNLIADAEAVERAGAYAVVLE"
     gene            complement(666..1631)
                     /locus_tag="VFV93_10355"
     CDS             complement(666..1631)
                     /locus_tag="VFV93_10355"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX5165786.1"
                     /translation="MRIVVVGSGAMGGLFGGKLAAAGNDVLLYDTWSEHVAAIERLGL
                     SIEGVDNTVVRYRVHATDQPPRDVADAELILVQVKGYDTYESLRPFNGRLNPETFVLS
                     LQNGLGNLEQMRQALPGHHRILLGTTAHGSTVLGPGRIRHAGKGATSIGDPAITATPR
                     FQLAPIRATLQGAGFDTSIADNVHSAIWAKLISNVVINPLTALTGLRNGDLLDDPRIV
                     AMMEAVIAESLGVMEAAGVPPAVEDYYQHARRVMEMTRPNVSSMLQDIRAGRRTEIES
                     INGAVARLGKELGVSTPLTDWLTAMVQAKERDAGRSRDSGAGIRG"
     gene            complement(1632..2885)
                     /locus_tag="VFV93_10360"
     CDS             complement(1632..2885)
                     /locus_tag="VFV93_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX5165787.1"
                     /translation="MEKDGTTLDQRIAAAQACLDTSGLLETLAGLVRIPSVFDPARPD
                     GNERDAATLIGGLLDDWALAYRTREVAPNRPNIVVDIAGTRPGPLLIFEGHTDVVTAG
                     DRAAWSIDPFAGEIRDGRLYGRGACDMKGGLAAMLFAAHAVEAAGRDFAGMIRLAILA
                     DEEGLMQGAKGFVADGELAGATAAIICEPEGDRVCIAQKGAIRIRVSLTGRMAHGAMP
                     EEGANPIAALGLVISRCRDLERQIQSEHPPHPLLGTFYLTPTVALAGEREQGNVIPSS
                     AELMLDIRTTPSNDHSSIIERVAAVIAAAAGEIAGVTQQIEIVDNRPATETDAKDPIV
                     RAVIAAHEAETGATPSFGGVPGSTDGTIFWAASRLPLVTYGPGITTLPHQADEYVELD
                     EVVRYARTYIAAAHRYFDVMERGSN"
     gene            3037..3564
                     /locus_tag="VFV93_10365"
     CDS             3037..3564
                     /locus_tag="VFV93_10365"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEX5165788.1"
                     /translation="MAFEQHHEFENLTRADAEAIGVPGQRRFRMIFGDELDAIVLWLE
                     KQQLQALGMAFEQMLAQLRAAGVDEARVQDIEPSPVGPIPPGTPEYTVGKLAVGFDEE
                     NVRVILFIHNIEAEEDAAPTFSGKLDLQRAATMAKQIETVVSAGRPTCPRCGAPIGPN
                     GHVCPHDNGHFPLNA"
     gene            3683..>4546
                     /locus_tag="VFV93_10370"
     CDS             3683..>4546
                     /locus_tag="VFV93_10370"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5165789.1"
                     /translation="MRSAVEGLLAGSGTPGAGVAVLIDGKLAFAGSVGAADLAGDVAL
                     LEDARFYVYSVTKTLLAALTLQSVERGDVDLDRAIQEYLPGYPLATSVTLRQVLNHTA
                     GLPDYGGMPEYVTAVRAHPKSAWSPQVFLDQTVANGLLFPPGEGWVYSNIGYLTIRLL
                     LERVSGLSFRTLVDRQIVEPLGLVATTVAETLDDTAALTPGYSGWIDPHGPVTDISRR
                     YHPGWVSHGVVISTASDLARIIDAIFAGRLAPSSLLPEMLTPVQVPMQHPLFVEPSYG
                     LGVMLDPGSRHG"
BASE COUNT          714 a         1429 c         1583 g          820 t
ORIGIN      
        1 ttcgaggacg acggcgtagg cgccggcgcg ctcgacggct tcggcgtcgg cgatcaggtt
       61 gcgggcggcc tccagcgagc ggccctgcac cttgaagccg cccagcgcgg ccgatgcctg
      121 cggtgtcagg ccgatgtggg ccatcacggg aatgccggcc cggacgatcg cctcaatccg
      181 cgaggccatc gactggccgc cttcgagctt gacggcgtcg gcaccgccct ccttcatcag
      241 ccggccggcg ttttccagcg cctgcgcgtc cgaaacctgg taggacatga acggcaggtc
      301 ggcgacgacg tggacgctcg tgacgccgcg catgacggcg cgggtgtggt ggatgatgtc
      361 ctccatcgtg accgggatcg tcgactcgta gccgaggacg accatcccga gcgagtcgcc
      421 aaccaggatc gactcgatcc cggccgcctc gaccgctcgc gctgtcgggt agtcgtaggc
      481 cgtgaccatc acaatcttct cgccgctcgc cttcatctcc agaatggccg gcgccgtgat
      541 cttctcgcgg gtgggtgggg tggtggacat tgggtgttct ccgttcgtcg tgttgtggtg
      601 gggattcggg tttcgggatt cggggctggt tgacgtgcga ctttcggggt gccgcgatct
      661 gtttcttatc cccgaatccc ggctcccgaa tcccgacttc gtcccgcgtc ccgttccttc
      721 gcctgaacca tcgctgtcag ccagtcggtg agtggggtgg agacgccgag ttccttgccg
      781 aggcgggcga cggcgccgtt gatcgattcg atctcggtcc ggcggccggc gcggatgtcc
      841 tgcagcatcg aggagacgtt cgggcgggtc atctccatca cgcggcgggc gtgctggtag
      901 tagtcctcga cggcgggcgg gacaccggca gcctccatga cgcccagcga ctcggcgatg
      961 acggcctcca tcatcgcgac gatgcgcgga tcgtcgagca gatcgccgtt gcggaggccg
     1021 gtcagagctg tcagcgggtt gatgacgacg ttggagatca gcttggccca gatggccgag
     1081 tggacattgt cggcgatgct ggtgtcgaag ccggcaccct ggagggtggc gcggatcggc
     1141 gcgagctgaa agcggggtgt cgcggtgatg gccgggtcgc cgatcgacgt tgcgcccttg
     1201 cccgcatggc gaattcggcc cggcccgagg acggtcgagc cgtgggcagt cgtcccgagc
     1261 aggatgcggt ggtgacccgg cagcgcctga cgcatctgtt ccaggttgcc gaggccgttc
     1321 tggagcgaga ggacgaacgt ctcgggattg aggcggccgt tgaacggccg cagcgactcg
     1381 taggtgtcgt agcccttgac ctggacgagg atcagttcgg cgtcggcaac gtcgcgcggt
     1441 ggctggtcgg tggcgtggac gcggtagcgg acgacggtgt tgtcgacgcc ctcgatgctg
     1501 aggcccagcc gctcgatggc ggcgacgtgc tcgctccagg tgtcgtagag gaggacatcg
     1561 ttcccggcag cggccagctt gccgccgaac agcccgccca tcgcgccgga accaacgacg
     1621 acaattcgca tctagttgct gccccgttcc atcacatcaa aatagcggtg tgccgccgca
     1681 atgtaggttc gcgcgtaacg aacgacttcg tccagctcga catattcatc ggcctgatgt
     1741 ggcaaggtcg tgatacccgg cccgtaggtc accagcggga ggcgtgacgc cgcccaaaag
     1801 atcgtgccat cggtcgagcc ggggacgccg ccaaatgacg gtgtcgcgcc agtttccgcc
     1861 tcgtgggcgg cgatcacggc ccggacaata ggatccttcg catcagtctc ggtcgctgga
     1921 cgattgtcga cgatctcgat ctgctgtgtg accccagcaa tctcgccagc ggccgcggca
     1981 atgacggcgg cgacgcgctc gatgatgctg gagtggtcgt tggagggagt ggtgcggatg
     2041 tcgagcatca gttcggcgct gctgggaatg acgttcccct gctcgcgctc accggccaag
     2101 gcgacggttg gcgtgaggta gaacgtaccg agcagcgggt ggggtggatg ctcggattga
     2161 atctgacgct ccagatcgcg gcagcggctg atgaccaggc cgagcgccgc gatcgggttg
     2221 gcgccttcct caggcatcgc gccgtgggcc atccggccgg tcaggctgac acggatacgg
     2281 atcgcgcctt tctgggcgat gcagacgcgg tcgccttccg gttcgcagat gatcgcggcc
     2341 gttgcgccgg ccagttcgcc atcggcgacg aagcccttcg cgccctgcat caacccctct
     2401 tcgtcggcga gaatcgccag ccggatcatt ccggcgaagt cgcgcccggc ggcttcgacg
     2461 gcgtgggcgg cgaacagcat cgcggccagc ccgcccttca tgtcgcaggc gccacgaccg
     2521 tagagacgac cgtcgcgaat ctcacccgcg aaggggtcga tcgaccaggc cgcgcggtcg
     2581 ccggccgtga cgacatcggt gtgaccttcg aagatgagca gcgggccggg gcgagtgcca
     2641 gcgatgtcga cgacgatatt gggacggttc ggcgcaacct cgcgggtgcg ataggcgagg
     2701 gcccagtcgt cgagcaagcc accgatcagg gttgcggcgt cccgttcgtt gccgtcgggc
     2761 cgggccgggt cgaagacgct gggaatccgc accagcccag ccagtgtctc cagcaggccg
     2821 gacgtgtcga gacacgcctg agccgccgcg atccgttggt ccagcgtggt gccgtccttc
     2881 tccaaccgaa aatcctccgc agcgccgttc cggccggttg ccgcgcctat cgtagcatgg
     2941 ccggcgcggc agtctttttg gcggcccgtg taccattgaa tcagcgaatc cggcatctgc
     3001 cggccgccgt tcgattccat agtgacaggt ctcagcgtgg catttgagca gcatcatgag
     3061 ttcgagaatc tgacccgcgc cgatgccgaa gcgatcggcg ttcccggcca gcgacgcttc
     3121 aggatgatct tcggcgacga gctggacgct atcgtcctct ggctcgaaaa gcagcagctt
     3181 caggcgctcg ggatggcctt cgagcagatg ctggcccagc tccgcgcggc cggtgtcgat
     3241 gaagcgcgcg tccaggatat cgagccgtcg ccggtcggtc cgatcccgcc aggaacaccc
     3301 gagtacacgg tcggtaagct ggctgtcggc tttgacgagg agaacgtccg cgtgatcctc
     3361 ttcatccaca acatcgaagc tgaggaagac gccgcgccga ccttctccgg caagctcgac
     3421 ctgcaacggg cggcaacgat ggcgaagcag atcgagactg tcgtctcggc cggccggcca
     3481 acctgcccgc gctgtggcgc cccgattggg ccaaacggac acgtctgccc acacgacaac
     3541 gggcatttcc cgctgaacgc gtaatcgttc tgtaacaggg cagcctgggt cgagagaagg
     3601 gccggcacga ggcgggccct tgcttcttgc atgtgaaggt gattggcgtg ggtcgatgac
     3661 ggacgatggt ttaggcgatc gcgtgcggag cgccgttgag gggttgcttg ccggttcggg
     3721 aacgccgggg gccggcgtag ccgtcctgat cgatgggaag ctggcgttcg ctggtagtgt
     3781 cggggcagca gacctggccg gtgacgtggc gttgctggag gacgcacggt tctacgtcta
     3841 cagcgtcacc aagacgctgc tggcggcgct gacgcttcag tcggtcgagc gtggcgacgt
     3901 tgatctggac cgggccattc aggagtatct gccgggttac ccgctggcaa cttcggtgac
     3961 gctccggcag gtactcaacc acacggccgg actgccggat tacggaggca tgccagagta
     4021 cgtgacggct gttcgcgcgc atcccaaaag cgcatggtcg ccgcaggtgt tcctcgacca
     4081 aacggtggcg aacgggttgc tgttcccgcc cggcgaaggc tgggtctact cgaacatcgg
     4141 atacctgacg atccggctgc tgctggagcg tgtcagcggc ctcagcttcc gcacgctggt
     4201 cgatcgtcag atcgtcgagc cgcttgggct ggtcgcaacg acggtcgcgg agacactgga
     4261 cgatacggcg gcgctgacgc cgggatacag tggctggatc gatccccatg gacccgtgac
     4321 ggacatctca cgccgctatc accccggctg ggtctcccac ggcgtcgtca tctcgaccgc
     4381 gtccgatctg gctcggatca tcgacgcaat cttcgctggc aggctcgcgc cgtcgtcgct
     4441 gctgccggag atgctgacgc cggtgcaggt gccgatgcag catccgctgt tcgtagagcc
     4501 atcgtacggt ttgggcgtca tgcttgaccc cggctcgcgg catggc
//
LOCUS       DASUGJ010000468         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1878903, whole genome shotgun sequence.
ACCESSION   DASUGJ010000468 DASUGJ010000000
VERSION     DASUGJ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878903"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFV93_10375"
     CDS             complement(<1..519)
                     /locus_tag="VFV93_10375"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5165790.1"
                     /translation="MQHNGALVATTPSAIKPTKADDAYEELKRLIVSLRIAPGEPLNE
                     RTLMSTMDVGRTPLREAIQRLTMEQLVTSLPRRGYFVRELSIPELNDMIAARKIVEPA
                     VTRMAVPNLTPDDITSLRSLVEVTRAELARNNHETGMYHDLEFHSIIANACGNRYLAA
                     VAIQINTRLLRYW"
     gene            858..>2269
                     /locus_tag="VFV93_10380"
     CDS             858..>2269
                     /locus_tag="VFV93_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5165791.1"
                     /translation="MEILERLRAGMMESRLTRRTVLLGTGAAMATGLLAACGGDDDDD
                     DDDGGTTGGDATSTTADSASGTSEAGSEGSPEAPEGDSEGEPVSGGTLKYGNYDEPPI
                     MDPRVSGATTAWRLFYCIFDPLVFQDVETGDFLPGLANTWEISEDGLVYSFTLKEGVK
                     FHDGTDFNADAIKFTYDSILDPELKSLAAIGYLGPYEKTEVIDDLTAEVHFTDPYAPF
                     LNNLSHSVLSPVSPTAVEKFGADFGTNPVGTGPFVFKEWQQQTSMTFTKNPDYNWGGG
                     VYTHEGPAYVDEIVVSFITEPTTLLGTLENGESDIINATPAQEVERLQGDDNYQILLP
                     RVPGSPQVLPMNAVKPPTDDLKVRQAIIQAVDMDTIVDTMWYGTRKAAQGPLSSPTWS
                     YNPVVETMYPYDPDAAGALLDEAGWTKGSGNVREKDGEKLHVTYITTAGSNGQAGELV
                     QAYLIEAGFEVDLQILEYAAT"
BASE COUNT          426 a          719 c          723 g          401 t
ORIGIN      
        1 ccagtagcgc agcagccggg tgttgatctg aatcgcgacg gcggcgaggt agcggttgcc
       61 gcaggcgttg gcgatgatgc tgtggaactc gagatcatgg tacatgccgg tctcatggtt
      121 gttgcgggcc agctcggcac gtgtcacctc gaccagggaa cggagcgagg taatatcgtc
      181 cggagtgaga ttcgggacag ccattcgggt gacagccggt tcgacaatct ttcgggcggc
      241 aatcatgtcg tttaattccg ggatcgacaa ttcacgaacg aaatagcccc ggcgcggcag
      301 actcgtgacc agttgttcca tcgtcagccg ctggatcgct tcgcgtagcg gggtccggcc
      361 aacgtccatc gtcgacatca gtgttcgctc gttgaggggc tcacccggcg cgatccgtag
      421 gctgacgatc agccgcttga gctcctcgta ggcatcgtcg gccttggtgg gcttgattgc
      481 tgatggcgtc gtcgccacca gtgccccgtt gtgctgcatc gtgatctgtt ccaatcgatt
      541 acgacatcgc cgtcgggccg cgtcgcgccg cacctgctgg tgagtggcct gcgtcaacag
      601 tactatccgg cgatcaaggg tagcatcata tgagtatgta gcgtaatata tcctgagtat
      661 tgaccagcgc acaccagtta gaacattgtg attgccgcca cattccggcg cacgatcgat
      721 gggctacctg tagtcgcacc tggtcagcgg cgccccggat ccggtgggac gcgaggcagt
      781 tgagagagct gctcgttcga tcggcgatgc ggtgcgtccg tgtacggctc gtagacacat
      841 tgagggggag aacgagcatg gaaatcctgg aacggctccg ggcggggatg atggaatccc
      901 gccttacccg ccgaaccgtc ctgctgggca ccggcgccgc gatggcgacc ggcctgctgg
      961 cggcctgcgg tggcgacgac gatgacgatg atgacgacgg cggcacgacc ggcggcgatg
     1021 cgacctccac aaccgccgat tcggcatccg gaacgtccga ggccggcagc gaagggtcgc
     1081 cggaggctcc cgagggcgac tccgagggcg agccagtctc cggtgggacg ctcaagtacg
     1141 gcaactacga cgagccgccg atcatggacc cgcgcgtctc cggcgcgacg acggcctggc
     1201 gcctcttcta ttgcatcttc gacccgctcg tcttccagga tgtcgagacc ggcgacttcc
     1261 tgcctggcct ggccaacacc tgggagatct ccgaggatgg cctcgtctac tcgttcacgc
     1321 tgaaggaggg cgtcaagttc cacgacggca ccgacttcaa cgcggacgct atcaagttca
     1381 cctacgacag catcctcgat ccggagttga agtcgctggc agcgatcggc tacctcggcc
     1441 cgtacgagaa gaccgaggtc atcgacgacc tgacggccga ggtgcatttc accgatccct
     1501 acgcgccgtt cctgaacaac ctctcgcaca gcgtcctctc accggtctcg ccaacggccg
     1561 tcgagaagtt cggcgccgac ttcggcacca acccggtcgg taccggcccg ttcgtcttca
     1621 aggagtggca gcagcagacc tcgatgacgt tcacgaagaa ccccgactac aactggggcg
     1681 gtggcgtcta cacgcatgag ggtccggcct acgtcgacga gatcgtcgtc tcgttcatca
     1741 ccgagccgac gacgctgctc ggtacgcttg agaatggcga gtccgatatc atcaacgcca
     1801 ccccagccca ggaggtcgag cggctgcagg gcgacgacaa ctaccagatc ctgctgccga
     1861 gggttccagg ctcaccgcag gtgctgccga tgaacgccgt caagccgccg acggacgacc
     1921 tgaaggtccg ccaggcgatc atccaggcgg tggacatgga caccatcgtc gacacgatgt
     1981 ggtacggcac ccgcaaggct gcccagggtc cgctctcctc gccgacctgg agctacaacc
     2041 cggtcgtcga gacgatgtac ccgtacgatc cggatgccgc cggcgctctc ctcgatgagg
     2101 ccggctggac aaagggctcc ggcaacgtcc gcgagaagga cggcgagaag ctgcacgtga
     2161 cctacatcac gacggccggc tcgaacggcc aggccggtga gctggtccag gcctacctga
     2221 tcgaggccgg cttcgaggtc gacctgcaga tcctcgaata cgcggcgac
//
LOCUS       DASUGJ010000469        10203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1878937, whole genome shotgun sequence.
ACCESSION   DASUGJ010000469 DASUGJ010000000
VERSION     DASUGJ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878937"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFV93_10385"
     CDS             complement(<1..604)
                     /locus_tag="VFV93_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5165792.1"
                     /translation="MALNRPIEPQTTADDTFLSLAQAADYLGLSQAALEAALGRGELR
                     AAGNRGHAQFDTHELDSFLDNQRARAVITGKLTMSLPMQIDVPRLLRAMVTLPDEQHA
                     AEALANEITALAGTCCKVTAVLRLDEATESVRLLALSDFPGRQRMMASAATSGERSEA
                     DLLDLPEGLRQAVVEHGGHHETLDIQPLLRPLMRAQARELI"
     gene            899..1429
                     /locus_tag="VFV93_10390"
     CDS             899..1429
                     /locus_tag="VFV93_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165793.1"
                     /translation="MASWMESVGYIASEPARVAVMVALVQSERPLEEMSIAQMAGLLH
                     QRITGTPLETAVLMRHIRDLAERGLLERDESGFRWQMTSLGVLVSRQWASGTLEPDGT
                     APLAEGEVRAWRDRLIDQMEQDANLADEAEIEREELLAGQGPRLAELRVLNRVLGDNR
                     LPDWLQEMAGGSDDEE"
     gene            1483..2553
                     /gene="add"
                     /locus_tag="VFV93_10395"
     CDS             1483..2553
                     /gene="add"
                     /locus_tag="VFV93_10395"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX5165794.1"
                     /translation="MLTEAERDLIRRMPKAELHVHLEGSVRPETLLDLGRLHGVAYPF
                     SDADGAREWFRFRNFPHFIEVYVAICNCLLTVDDYDRVTYELALEAHRQQIRYLEVTI
                     APASLIEPRNSAVPDVVIAGCRAGARRALADLGVRMQFILDAVRSRPVEHVMALAEWT
                     AANQGDGLVGLGLGGQEVGYPAMPHAAAIRLARDRGVRISLHAGETVGPESVWDALEA
                     GSERIGHGVTSIHDPALVGHLVERGIVLEVSPTSNLCLGVAPSYVEHPFRALYDAGVT
                     LTVNTDDPPMFGATLTGEYLALADHCGFTLDELVELNLNAARAAFLPEAERASLVASF
                     ESEIAGLREELDAPARSRDTVG"
     gene            2800..3570
                     /locus_tag="VFV93_10400"
     CDS             2800..3570
                     /locus_tag="VFV93_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX5165795.1"
                     /translation="MAHNGRIPHDTAITIGAGHHDIPDIVIRRLPIYVRTLKAIHESG
                     IATVSSDDLAEQIGVTAAQIRRDLSYFGKFGKQGKGYDVPTLVSEIEHILNLDRQWDV
                     ALIGYGHLGQAIAAYKGFDATAFRISAIFDRHPKHFGELSEDGVPVLPDTEISRVVRE
                     KHIRLGIVAVPASSAQEVTDALIAGGVTAILNYAPAILRVPDGVWVREVDPTSAMQSM
                     TYYLVDRTGDDDDRISVLSPDLDDETPNGKSIEAADGM"
     gene            complement(3701..5965)
                     /locus_tag="VFV93_10405"
     CDS             complement(3701..5965)
                     /locus_tag="VFV93_10405"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX5165796.1"
                     /translation="MTSLNLRPACGGWRIRAALLIPLLVSLLAIAGLRPEQVEAHAFL
                     ERSEPSANRVLPEQPAEVSLTFTEPVEPEFSKAELYDATGALIPTDPSQIVAANQIVL
                     TLPAGLPNGTYTVQWRNVSASDGHPQQGFVPFTIGSQADVVTPSPPQAINFSAPPAWL
                     ETTGRWLSLLGLTGAAGAIVCWLWVIRQARDPLDDEFYDRVRDRVAQLIMVAAGVGVA
                     GSLIALGVQAKDSGASFSMGTIIDILTDTRYGHLWIARVLLLLGLAVLATFDSLWDEE
                     PAITTIVLALGLAAGAMLPFSLNSHAAAQPIGADTAVAVDWLHLAASSVWIGGLLALL
                     ITIILGTRGAPADQRRQVFATAVPRFTTLATISVIVLALTGFYSAWLQVGNLLALRET
                     SYGQTLIVKLALLVPLLILGAVNMRIIGPKLRTAASAGLHFGRTVAAEAALGIGILVA
                     VGLLTSLPTARDTITEDAQNTIFRFIENDIYAVLYITPGSAGFNRYTADIAIDGMDNV
                     SSAQLLLRLEKEGDVEGIREIPLQYKFGNRFEASGADLSVTGDWNVEFIVRREGMADA
                     RFKEQINVPSTPPADRVPGQPPRFLGTTSTAAVLFAGLAIIAIVGSLRAPGVWQDRVI
                     GTGIGAALLLAGGLILLVNKDDPTPTALAANPIPLTEASIEAGHEVFLANCTSCHGQT
                     GQGDGPEAANLNPPPANLTEPHVAVHTDGDLHWWITNGIKPSMPPFGEALTEEEIWNV
                     INYVRSLSGSAASP"
     gene            complement(5976..6716)
                     /locus_tag="VFV93_10410"
     CDS             complement(5976..6716)
                     /locus_tag="VFV93_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165797.1"
                     /translation="MRPPGDWPARTTYAAGALPDLRYEGRSRQMNVTIIRGLLAVALS
                     AMLMLGLAATASAHEHREVGDYELTVGFTGEPALVNEPNGLDLRVQKGHGDDGTPVEG
                     LQETLKAEISFAGETKELELRARFGQPGAYTADIFPTAEGAYTFHIFGTIEGTDVDET
                     VTSGPETFAEVESTDAIAFPSTTGADSGANAVSDAQDSADSAQTLATAAIIVGVLGLI
                     AGLGGAIMAMNARKTNRTAGTVTPETGD"
     gene            complement(6736..7137)
                     /locus_tag="VFV93_10415"
     CDS             complement(6736..7137)
                     /locus_tag="VFV93_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165798.1"
                     /translation="MRVRYLDILIYLTRLGASTLIMPAVLATALLSFPTTCTCGAEYP
                     HEHPLFGITGHHHGPGGEADSQQRRSDVSRASVDGPSVQASSGAAALSLTAIPTGSAH
                     LQSIPRPLLQFGITNAPDGAHATPDVPPPQA"
     gene            complement(7492..8091)
                     /locus_tag="VFV93_10420"
     CDS             complement(7492..8091)
                     /locus_tag="VFV93_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165799.1"
                     /translation="MGILPEHAAIPVEWRTQDLWLRVLRPDVTELDFAALMESRERLR
                     LWSDSTWPSDDFTLQENERDLIEHEREWEDREAFAYTVLNSDGRRCEGCVYVTPFASS
                     MRHRGYDPIAQGTPFAEDAAAVSFWVRDSALARDLDRQLLAGLREWFQTEWPLSQVIY
                     RINDNQHHDRQLFEEAGLVHRASHGTSRSPLIWHLYTEP"
     gene            complement(8167..>10203)
                     /locus_tag="VFV93_10425"
     CDS             complement(8167..>10203)
                     /locus_tag="VFV93_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5165800.1"
                     /translation="EGTAYDLGSALIGAAAGVAVAFAIAIVMGELIGDFLSITPHATF
                     RSLVVAYSLGVTVTFITIIFASVRSSRLNIVAAIRDLPDNSAPEARKRPRWRWWSKLP
                     RFAGTASAIVWLIPEFIWNVLLIPVKLVVWLLRTLANYVGWGPALTLLGVLLLLTGTS
                     NKSVFQFSVGLAVGSLGIALFLKRYLPARPVYTIIAALNLAYYLVPFDKLDVILPDLG
                     TGGFEMFFVSGIAMVAFSTLIIMWNADVLVGFMGLVGRLATRWLPALKTAVAYPLASK
                     GRTGLTIAMFAMVIFSLVTLTTINSNFIKLFSTEEADAGWDVRVETSPSNPIDNLEAS
                     LQGSEVDVSHVAAVGKIDGIDYQNTRVRTPGDEDWGLYTLGGINPAYIDGADIPLEAR
                     APGYDSDAAVWDALRSGQPVALADSLIFSSDEFGNDPDSYAAPKDAEIVDGAIPAIPL
                     ELQSDRTGKTETVTIIGIIDSNVSTLFGIYLPEQVFTDLYGAPDFTNLFVRMDDHPSP
                     EAEDVAKQIESTLLTSGVQAESIQKEIDESVGFQTGFFKLLQGFMGLGLFTGIAALGV
                     ISFRSVVERRQQIGMLRAIGYRRNMVALSFMLESLVVAALGVLSGTVLALILSYNLIN
                     SEDFQEGAEFSGFVIPWGTVLFFIGASIVAAAIMTWVPARKASSVPIADALRYE"
BASE COUNT         1707 a         3213 c         3525 g         1758 t
ORIGIN      
        1 cgatcagttc gcgagcctgg gcgcgcatca gtggtcggag cagcggctga atgtccagcg
       61 tctcgtggtg cccgccgtgc tcgacgaccg cctgccgcaa cccctctggc aggtcgagca
      121 gatcggcttc cgaccgctcg ccgctcgtgg ccgcagacgc catcatccgc tgccggccgg
      181 ggaaatcgct gagcgccagc agccgaactg attcggtcgc ctcgtcgagc cgcaggacag
      241 ccgtcacctt gcagcaggtg ccggccagtg ccgtgatctc attggcgagc gcctcggcgg
      301 cgtgctgctc gtccggcaac gtcaccattg cccgcagcag cctggggaca tcgatctgca
      361 tcggcagcga catcgtcagc ttgccggtga tgactgccct ggcgcgctgg ttgtcgagga
      421 aggagtccag ctcgtgggtg tcgaattgcg cgtgtccccg gttgccggct gcccgcaact
      481 ctccgcgccc gagcgcagcc tcaagtgccg cctggctcag gccgaggtag tcggcggcct
      541 gcgccagcga caggaatgtg tcgtcagccg tggtctgtgg ctcgattggc cggttcaatg
      601 ccaagagacc ccctgggccg agtgctgcgc tgcggtggcg tttcccagtc gtcagtacgg
      661 agaatgtcct gcatcgtatt atgcggcagg atcgcgcggc agccgcatgc ccgaaagtac
      721 ggaatctggc tagcaaatcg tcttagtgtg gccgggcaat cgggacataa tttgagtatg
      781 gcagcgcgca gcggtgtacg gcgggcgagc gatgcctacc gtcgcggcat ccggctcggt
      841 aggatagaga gccacgcgcg ggcgacaacg gcaggcaagc ggggggagcg aaacgcagat
      901 ggcctcatgg atggagtcgg tcggctacat tgccagtgag ccggccaggg ttgcggtcat
      961 ggtggcgctg gtccagtcgg agcggccgct ggaagagatg tcgattgccc agatggcggg
     1021 gctattgcat cagcgcatca ccggcacgcc gttggagacg gccgtcctga tgcggcatat
     1081 ccgcgatctg gccgagcgcg gcctgctgga gcgggacgag tccggctttc gctggcagat
     1141 gacgtcgctg ggtgtgctcg tcagccggca gtgggcctcc ggcacgctgg agccggatgg
     1201 caccgcgcca ttggccgagg gcgaggtacg cgcctggcgc gaccggctga tcgaccagat
     1261 ggagcaggac gccaacctgg ccgacgaggc cgagatcgaa cgtgaggagc tgctggccgg
     1321 tcagggacca cgcctggccg agctgcgcgt cctcaatcgc gtcctcggcg acaaccgcct
     1381 gcccgactgg ctccaggaga tggccggcgg cagcgacgac gaggagtagg acgttctgac
     1441 gccgattgcc ggcgattgat gacggttgag agggctggtg cggtgctgac cgaggcggag
     1501 cgcgatctga tccggcggat gccgaaggca gagctgcacg tgcatctgga gggaagcgtc
     1561 cggccggaga cgctgctcga cctgggccgc ctgcacggcg tcgcctaccc gttctctgat
     1621 gcggacggcg cccgtgagtg gtttcgcttc cgcaacttcc cccacttcat cgaggtctat
     1681 gtcgcgatct gcaactgcct gttgacggtc gacgactacg accgcgtgac gtacgagttg
     1741 gcgctggaag cgcaccgtca gcagatccgc tacctggaag tgacgatcgc gccagcctcg
     1801 ctgatcgagc cgcgtaacag cgccgtcccg gatgtcgtca tcgccggctg ccgtgctggc
     1861 gcgcggcggg cgctggccga tctcggcgtc cggatgcagt tcatcctcga tgccgtccgc
     1921 agccggccgg tcgagcatgt catggcgctg gccgagtgga cagccgcaaa ccagggcgac
     1981 gggctggtcg gtctcgggct gggcggccag gaggtcggct acccggccat gccgcacgcc
     2041 gccgccattc gcctcgcccg cgatcgcggc gtgcggatca gcctgcatgc cggcgagacg
     2101 gtcggccccg agagcgtctg ggatgcgctc gaagccggct cggagcggat cggccacggc
     2161 gttaccagca tccacgatcc cgcgctcgtc gggcatctcg tcgaacgcgg gatcgtcctt
     2221 gaggtctcac cgaccagcaa tctctgcctc ggtgtcgctc catcttatgt cgagcatccc
     2281 ttccgcgccc tctacgacgc cggcgtgacg ttgacggtca acactgatga cccgccgatg
     2341 ttcggcgcga cgttgaccgg cgaatacctg gcgctggccg accactgcgg cttcacgctg
     2401 gatgagctgg tcgagctgaa cctcaacgcc gcccgtgccg cgttcctgcc cgaggctgag
     2461 cgcgcgagcc tcgtcgccag cttcgagtcg gagatcgcgg gcctgcggga ggagctggac
     2521 gcccctgccc gatcccgtga tacggttggg tagtcgggaa ctgcgcgcca gcccgcacac
     2581 cccaaagtgc gcaagattga aagcaagcgg tcgcgaatgg tagcatttct catcgtcttt
     2641 ttggcccggc acatctgacc ccaacggcca ggcgtgcgat tcgcgctggc tttcagggag
     2701 cgtgcccgga acagcgcggt attgcggcat ctttattgat gaggtttcag cggcggcgtg
     2761 atgagccgcg tcgtgccagt tagtaacagg tagtgaccga tggctcataa cggacggatt
     2821 cctcacgata cggcgatcac gatcggcgcc ggccaccacg acatccccga catcgtcatc
     2881 cggcgtcttc cgatttatgt ccgcacgctg aaggcaattc acgaatccgg catcgcgacc
     2941 gtctcgtcgg acgatctggc cgagcagatc ggcgtcacgg cggcccagat ccggcgcgac
     3001 ctctcctact tcggcaagtt cggcaagcag ggcaagggct acgacgtccc gacactggtg
     3061 agcgagattg agcacatcct caacctcgac cgtcagtggg atgtcgcact gatcggatac
     3121 ggacatcttg ggcaggcaat tgctgcctac aagggcttcg atgcgacggc gttccggatc
     3181 agcgccatct tcgatcgcca ccccaagcat ttcggcgagc taagtgagga cggcgtcccc
     3241 gtcctgccgg acaccgagat ctcgcgggtc gtgcgcgaga agcacatccg gctcggcatc
     3301 gtcgctgtcc cggcctcctc cgcgcaggag gtcaccgacg cgctgatcgc cggcggcgtg
     3361 acggccatcc tcaactacgc gccggccatc ctgcgggttc ctgatggcgt ctgggtccgc
     3421 gaggtcgatc ccaccagcgc gatgcagtcg atgacttact atctggtcga ccgcaccggc
     3481 gacgacgatg accgcatctc cgtcctctca cccgatctcg acgacgagac tcccaacgga
     3541 aaatccatcg aagctgctga cgggatgtaa cggcgtcgcc aacggaccat cccgatcacc
     3601 caatgcccaa tccggtacgg gcgatattgc gatatcgccc gtaccggatt gggcattgaa
     3661 ttgagccagc cggcgaactg gcgactgacc caacgtagcg tcacggcgat gccgctgatc
     3721 cgctgagcga gcgaacgtag ttgatcacgt tccagatctc ctcttcggtc agggcctcac
     3781 cgaatggtgg catcgagggt ttgatgccgt tcgtgatcca ccagtgcagg tcgccatcgg
     3841 tgtggacggc gacgtgcggc tcggtcaggt tggccggcgg cgggttgagg ttggcggctt
     3901 ccgggccgtc gccttggccg gtctggccgt ggcaggaggt gcagttcgcc aggaagacct
     3961 cgtggccggc ctcgatcgac gcttccgtca gcgggatcgg gttggcggcc agcgctgttg
     4021 gtgtcggatc atccttgttg acgagcagga tcaatccacc ggccagcagc agggctgcgc
     4081 cgatgccggt gccgatgaca cggtcctgcc agacgccagg cgcccgcagc gagccgacga
     4141 tcgcgatgat cgccagcccg gcgaacagga cggccgccgt cgaggtcgtt cccaggaagc
     4201 ggggcggctg gccgggaacc cggtcggccg gcggggtact ggggacgttg atctgctcct
     4261 tgaagcgcgc atctgccatg ccctcacgac ggacgatgaa ctcgacgttc caatcgccgg
     4321 tgacgctcag gtcggcgccg gaggcttcga agcggttccc gaacttgtat tggagcggga
     4381 tctcgcggat accctcgacg tcgccttcct tctccagccg cagcagcagc tgggccgacg
     4441 agacgttgtc catcccatcg atcgcgatat cggccgtgta gcggttgaag ccggctgaac
     4501 ccggcgtgat gtagaggacg gcgtagatgt cgttctcgat gaagcggaag attgtgttct
     4561 gggcgtcctc ggtgatcgtg tcgcgggcgg tcggcaggct ggtcagcagg ccgaccgcga
     4621 cgaggatgcc gatgccgagg gctgcttcgg ccgcaaccgt ccggccgaag tgaaggccgg
     4681 ccgaggcagc tgtgcgcaac ttcgggccaa tgatgcgcat gttgacggcg ccgaggatca
     4741 gcagcggcac cagcagcgcg agcttgacga tcagcgtctg cccgtagctg gtctcgcgca
     4801 gagcgagcag gttgccgacc tgcagccagg ccgagtagaa gccggtcagc gcgaggacga
     4861 tgacactgat cgtcgccagc gtcgtgaagc gcgggacagc ggttgcgaag acctggcggc
     4921 gctggtcggc cggcgcgccg cgcgtgccga ggatgatcgt gatcagtagc gccagcagac
     4981 cgccgatcca gacgctggag gcagccaggt ggagccagtc gacggcgacg gcggtgtcag
     5041 cgccgatcgg ctgagcggcg gcgtggctgt tgagcgagaa cggcagcatc gccccggctg
     5101 cgagaccgag cgcgagcacg atcgtcgtga tcgccggttc ctcgtcccag agactgtcga
     5161 aggtcgccag gacagccagc ccaagcagca gcagcacccg cgcgatccag aggtgtccgt
     5221 agcgggtgtc ggtcaggata tcgatgattg ttcccatcga gaacgatgcg ccgctgtctt
     5281 tggcctgcac gccgagcgcg atcaggctgc cggcgacgcc gacgccggct gcgaccatga
     5341 tcagctgcgc gacacgatcg cgaacgcggt cgtagaactc gtcgtcgagg ggatcacgtg
     5401 cctggcggat gacccagagc cagcagacga tcgcgccggc cgcgccagtc agcccgagca
     5461 atgagagcca gcggccggtc gtctccagcc aggccggcgg ggccgagaag ttgattgcct
     5521 gcggcggact gggggtgacg acatccgcct ggctaccgat cgtgaagggc acgaagcctt
     5581 gttgggggtg gccgtcggac gccgagacgt tgcgccactg gacggtgtag gtgccgttcg
     5641 gcaggccggc cggcagtgtc agcacgatct gattggcggc gacgatctgg ctgggatcgg
     5701 tcgggatcag cgcgccggtg gcgtcgtaga gttcggcctt cgagaactcc ggctcgaccg
     5761 gttcggtgaa ggtcaggctg acctcggcgg gttgctctgg caggacgcgg ttggccgacg
     5821 gctcgctgcg ttccaggaaa gcgtgtgcct cgacctgttc tggccgtagt ccggcaatcg
     5881 ccagcaggct gacgagcagc gggatcagga gggcggcgcg aatgcgccat cccccgcagg
     5941 caggacgaag gttcagggaa gtcatgtcag gctcgttagt cgcccgtttc aggcgtgaca
     6001 gttccggcgg ttcggttggt cttccgtgcg ttcatcgcca tgatagcgcc acccagcccg
     6061 gcaatgagcc cgagaacgcc gacgatgatc gcggcggttg ccagtgtctg ggcgctatcg
     6121 gcgctgtcct gggcgtcgct gacggcgttg gcgccgctgt cggcgccggt cgtcgatggg
     6181 aaggcgatgg cgtcggtcga ttcgacctcg gcgaacgtct ccggcccgct cgtgactgtc
     6241 tcgtcgacgt cggtgccttc gatcgtgccg aagatgtgga aagtataggc gccttcggcg
     6301 gtcgggaaga tatcggcggt gtatgccccg ggctggccga agcgggcgcg cagttccagc
     6361 tccttggtct cacccgcgaa gctgatctcg gccttgagcg tctcctgcaa gccttcgacc
     6421 ggcgtgccgt cgtcgccatg gcccttctgg acgcgcaggt cgagcccgtt cggctcgttg
     6481 acgagcgccg gttcgccggt gaagccaacc gtaagctcgt agtcgccgac ctcgcggtgc
     6541 tcgtgggcgc tggcggtcgc cgccaggccg agcatcagca tcgctgacag cgcaaccgcc
     6601 agcagacctc gaatgatcgt cacgttcatc tgtctcgatc gcccttcgta gcgcaggtcc
     6661 ggcagggcgc cggctgcgta ggtggtccgc gccggccagt cgccgggcgg acgcatgtgt
     6721 ctgcaatctc agttcttacg cttgcggcgg cggcacgtcg ggcgtggcgt gtgcgccgtc
     6781 gggtgcgttg gtgatgccga attgcagtag cggccggggg atggactgga ggtgtgcgct
     6841 gccggtcggg atcgcggtca gcgacaaggc agccgctccg gacgacgcct gcacggaggg
     6901 accatcgacg ctggcgcggg agacgtcgct gcggcgctgc tgggagtccg cctcgcctcc
     6961 ggggccgtgg tggtgaccag tgatgccgaa caggggatgc tcatgggggt actcggcacc
     7021 gcaggtgcag gttgtcggga acgagagtag cgccgtcgcg aggacggccg gcatgatcag
     7081 cgtgctggct cccaggcggg tcaggtagat caggatgtcg aggtaacgta cgcgcatcag
     7141 tgaactcgcc ggtccgatcg taccgtccgg gccgtctcgc cctgttgagt ctacccgaac
     7201 ccacggcaag gaacgaggcc gggcgattca ctgtctggta cgtcgcgggc ttgttggctg
     7261 gtttgtcggg cagttgttcc ctgtcatccc gacgcacggg ggatctcgtt ctcgttcgat
     7321 acctgccgcg tcaacgagtc acgagatcct tcgcttgcgc tcaggatgac aaggggaaca
     7381 tggacggtcc tcacgggcgc gtcgagtctt gcgtgcgtct gggcgctgat gtttcacgtg
     7441 gaacacgaaa gccgtcatcg tgcatccgcg tttcacgtga aacattcgcg cctacggctc
     7501 ggtatacagg tgccagatca atggagacct gctggtcccg tgggaggcgc gatgaacgag
     7561 accggcttcc tcgaacagtt gcctgtcgtg atgctgattg tcgttgatgc ggtagatcac
     7621 ctggctgagc ggccactctg tctgaaacca ctcacgcagg ccggccagaa gctgccggtc
     7681 caggtcacgc gcaagagcgc tgtcgcgtac ccagaaggag acggcagcgg cgtcctccgc
     7741 gaacggcgtg ccctgggcaa tggggtcgta ccctcgatgg cgcatcgaac tggcgaacgg
     7801 cgtgacgtag acgcagccct cgcatcgacg gccgtcggag ttcaggactg tgtaggcgaa
     7861 tgcctcgcgg tcttcccact cacgttcgtg ctcgatcagg tcgcgttcgt tctcctgaag
     7921 ggtgaagtca tcgctgggcc aggtcgagtc gctccagaga cgcaaccgtt cgcggctctc
     7981 catcagcgca gcgaagtcca gctcggtgac atctggccgc agcacccgta gccagagatc
     8041 ctgcgttcgc cattcgaccg gtattgcagc gtgttctgga agaattccca ttgtctgcct
     8101 ccctcagtaa cgacgaggag gcgggcatga tacccgcctc ctgtctgaac ttcgacgtgc
     8161 tgggcgctac tcgtagcgca gcgcgtcggc gattggcacc gacgaggcct tgcgggccgg
     8221 cacccaggtc atgatcgcgg cggcgacgat cgatgcgccg atgaagaaca ggaccgttcc
     8281 ccacgggatc acgaagccgc tgaactcggc gccttcctgg aagtcctcgg agttgatcag
     8341 gttgtacgac aggatcaacg ccaggacagt gccgctgagg acacccaggg ccgcgacgac
     8401 cagcgattca agcatgaacg agagcgccac catgttgcgc cggtagccga tcgcccgcag
     8461 catgccgatc tgctgccgcc gctcgacgac cgagcggaac gagatcacgc ccagcgccgc
     8521 gatgccggtg aagaggccga ggcccatgaa gccctgcagc agcttgaaga agccggtctg
     8581 gaacccgaca ctctcatcga tctccttctg gatcgactcg gcctggacac cgctcgtcag
     8641 cagcgtcgat tcgatctgct tggcgacatc ctcggcttcc ggcgacggat ggtcgtccat
     8701 gcggacgaac aggttggtga agtccggcgc gccgtagagg tcggtgaaga cctgctccgg
     8761 caggtagatg ccgaacagcg tgctgacgtt cgagtcgatg atgccgatga tcgtcaccgt
     8821 ctcggtcttc ccggtccggt cgctctgtag ctccagcggg atggccggga tcgcgccatc
     8881 gacgatctcg gcgtccttcg gggccgcgta gctgtccggg tcgttgccga actcgtcgct
     8941 ggagaagatc aggctatcgg ccaacgcaac cggctggccc gaacggagcg cgtcccagac
     9001 ggccgcgtcg ctgtcgtagc ccggcgcgcg cgcttcgagc gggatatcgg cgccgtcgat
     9061 gtaggcgggg ttgatgccgc ccagcgtgta gaggccccag tcttcgtcgc ccggagtccg
     9121 gacgcgggtg ttctggtagt cgatcccgtc gatcttaccg acggcggcga cgtgcgagac
     9181 atcgacctcg ctgccttgca gcgatgcctc caggttgtcg atcgggttgg acgggctggt
     9241 ctcgacgcga acgtcccagc cggcgtcggc ttcctcggtc gagaagagct tgatgaagtt
     9301 gctgttgatc gttgtcagcg tcaccagcga gaagatgacc atcgcgaaca tcgcgatcgt
     9361 caggccggtg cggccctttg acgccagcgg gtaggcgacg gccgtcttga gcgccggcag
     9421 ccagcgggtc gccagccgtc cgaccaggcc catgaacccg accaggacgt ccgcgttcca
     9481 catgatgatc aacgtcgaga aggcgaccat cgcgatgccg gagacgaaga acatctcgaa
     9541 gccaccggtg ccgagatcag gcaggatgac gtccagcttg tcgaacggca ccaggtagta
     9601 ggccaggttg agggcggcga tgatcgtgta gaccggtcgc gccggcaggt agcgcttcag
     9661 gaagagggcg atgcccagcg acccaaccgc cagcccaacc gagaactgga agacgctctt
     9721 gttgctcgtg ccggtcagca gcagcagcac gcccagcagc gtcagggccg gaccccagcc
     9781 aacgtagtta gccagcgtcc gcagcagcca gacgaccagc ttgaccggga tcagcaggac
     9841 gttccagatg aactccggaa tcagccagac gatcgcgctg gcggtgccgg cgaagcgcgg
     9901 cagcttgctc caccagcgcc agcgtggccg cttgcgggct tccggcgcgg agttgtccgg
     9961 caggtcgcgg atggcggcga cgatgttgag ccggctggag cggactgagg cgaagatgat
    10021 cgtgatgaac gtgaccgtca caccgagcga gtaggcgacg accagggagc ggaaggtcgc
    10081 gtgcggcgtg atactgagga agtcgccgat caactctccc atcacgatcg cgatcgcgaa
    10141 cgcgacagcg acgccggccg ccgcgccgat cagcgccgag ccgaggtcgt aggccgtccc
    10201 ttc
//
LOCUS       DASUGJ010000470        10740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1879087, whole genome shotgun sequence.
ACCESSION   DASUGJ010000470 DASUGJ010000000
VERSION     DASUGJ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879087"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VFV93_10430"
     CDS             <1..1101
                     /locus_tag="VFV93_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165801.1"
                     /translation="GWLGPEALLPRAGTGSDGSRTLASVELTEVDLDRLSIDARSTAS
                     GGDYFVASERGSGVPLIWNVDGNRPRRLTSDITDLAWFNHGTAAIGVAAGQAGDSGSR
                     IVVTAADIASTTAYLDHQYFDPANLGSSADQRYARPMVSPAGALVSFFVVDRLSGDVS
                     LWLDTGEGAAEVVAGWTLPVQRALDPPLVATWASPDTLLFAMPADWHDGLPQTTAFMR
                     LTLSSDTPQLDELARIDASRGAKGIVLAELALSPDGSLLAYRLRQFDSVSGSNATEDT
                     LHVAGTDNLNQAIELERGGSSDGLLWTTAGDQLVAGIRGRIALYSADGRDLAYLSPRG
                     VHASYPLLIGRQVWFEAVDDRGERVWQVDLEE"
     gene            1192..2106
                     /locus_tag="VFV93_10435"
     CDS             1192..2106
                     /locus_tag="VFV93_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5165802.1"
                     /translation="MARPLKVGFILPQIEGRRSGTTPRWSEIREMAEVSEQMGFDSLW
                     VVDHLLYGFGKDDPVRGVWEGWSLLAALAAVTTRPELGTLVVCTGFRSPALLAKMADT
                     VDEISDGRLILGIGAGYFEREYNAFGIPYDHRFSRFEEALEIIAGLLKHGEIDYEGQY
                     YFARESELRPRGPRPGGPPIMVGTRGEKMLRLTAKHADAWNGWLARGDNRPAAIPPLR
                     ADVDAACVEVGRDPATLDRTATVFVDPTDRAAQSSRGPVSGSPEEIAATLAAFADEGI
                     THIQIASKVTRVQELEPFASVLEALDRL"
     gene            2329..3420
                     /locus_tag="VFV93_10440"
     CDS             2329..3420
                     /locus_tag="VFV93_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165803.1"
                     /translation="MLVVVLLAAGVVACSGPDDSDSETATSAVEQGGSSGTAAGQPTE
                     TSGDVPAETPSATTDPVHTAGSTTDAQPTATTDPVPPTTAAPTEAVAPTPTAPVAQAG
                     GLLPNNRILSYYGHPNSAAMGILGEYTMEDAHAHLAEQAAEYEAADPAHPVTIAFELI
                     ATVAQPWPGDDGTYVAYTGDEIIQEYVDYVTANNMILILDLQIGLDTIPNQINMIRHW
                     LEYPNVHVALDPEFSMKGNEIVPNDRIPGEFIGEANGIHIQEGMNLVSQIVAEAGIPP
                     KLFIVHQFEEDMIFNKDQITPVAGVQFVLDMDGFGGSDAKLGNYTHFVHDQLIEFGGI
                     KLFYKQDDPLLTPPEIVALDPPALVVIYQ"
     gene            3462..4556
                     /locus_tag="VFV93_10445"
     CDS             3462..4556
                     /locus_tag="VFV93_10445"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX5165804.1"
                     /translation="MELSERAAALHQRAIVIDGHSDILLPVVMGTTTLTTPFPESELE
                     RWQRLVEVQQPARRSSDVPYGLDPLAIAIAPSGQYELPLLEAGGVTAECVALFLPETM
                     LDNALESALEMVAALHREIEANADRCLLVRSVADLQRAKAEGKVAYILTMEGAEPIGT
                     RIDLLDIFWRLGLRMTTLTHSRRNLLADGTQQDINTGGLTRAGREAVCRCQELGIVID
                     TAHISDRGFWDLIELVDGPILCSHTSVLTLTPGYRAPWDEINPTYGMSKAEAIARKGG
                     LIGVVFWSMADTAALVREVERITELTGPDHVGLGTDFFGFRDAPSDLQHIGQLPALTE
                     ALVQAGLDDDTILKVLGGNYLRIFEQAWRD"
     gene            complement(4730..5284)
                     /locus_tag="VFV93_10450"
     CDS             complement(4730..5284)
                     /locus_tag="VFV93_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX5165805.1"
                     /translation="MEVIEADIADVAAIGCEAYVNAANNELWMGAGVAGALKRAAGAE
                     VEQEAVRQGPIAVGEAVITSAGKMPPPARAIIHAAAMGFTDRTQIYASTETVAAATRR
                     ALQLCDEQALDSVAFPALGTGVGGLDIDACAKAMVAAVREHLLAGSSLQRVVFVVRGS
                     GATGLAPRAEVFRDEIRKQFSGDR"
     gene            5406..5999
                     /locus_tag="VFV93_10455"
     CDS             5406..5999
                     /locus_tag="VFV93_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165806.1"
                     /translation="MTLWKSSALIAFVLVLLLAGCGGDGRPDPTSTVKTTAEPGDPHV
                     LEPMTTGRDIVAPDGRYSLRIPGEWVEYDDPFAELSFRTVAEDPALAINVNRDEIDES
                     TRVQVYAEEARARISRTYRNVNSLSLTPVKIGSMEAYRWIYTAAVGTIEHLFYQVFLI
                     DAGEGFVLTGSAPIDVDIDLVRPIFDGIAGSMTFARG"
     gene            complement(6011..6319)
                     /locus_tag="VFV93_10460"
     CDS             complement(6011..6319)
                     /locus_tag="VFV93_10460"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX5165807.1"
                     /translation="MNTDEEQDQPAQPISNAGVERALGSLPGWRREEGGIRKRYRRAS
                     FRDAIAFVNQIADLAEAAGHHPDIDIRWRTVILFLTTHEAGGITNLDLELARQIEDAS
                     "
     gene            6424..9180
                     /locus_tag="VFV93_10465"
     CDS             6424..9180
                     /locus_tag="VFV93_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5165808.1"
                     /translation="MSSRRRYISRANRLRSTRRGQSGYGARPGSPARRVSPRLLATHD
                     LRQRRDQGERVTSMARIASVAAIFAATLFVGFAIASASAAVAAWNYFSEDLPSIADFE
                     ARQFETTRIYDRNMNLLYEVSDPETGWRTYVPLREITANDTNTFIVDATVAAEDETFW
                     KNYGVEPAAILRGAFINVSGRGSSGGSTITQQLVRQLYPEKIGFERSYDRKIREAIVA
                     FQFTQHYSKEQILEMYLNSIFYGNRAYGIEAAAQAYFNKHPSELTLAEASMLAGLPQA
                     PSYYDPTVNMEAAKTRQRYVLDRMAHDGYITQQQADAAWAEILIPQARDSQLNPAPHW
                     VNFVIDHLEARYGAEKVYRGGLTVRTTLDLGLQLEAEAAVRKHLDTLGPYNATNGALV
                     ALLPGTGEVVAMVGSRDYSDDTISGQVNVAVSERQPGSAFMPITYAAAFEQGWYPGTT
                     ILDYQTQWQTPGAPEPQYVPENTSRTYNGAVTSRQALAGSLNIPAVKAIDYAGVENTI
                     DLAHRIGIREGLWRGVGVYGLALTLGGGEVSLLELTNSYATFANTGRYVGYNPVLEIT
                     GPDGETIDRADPGSALPNAPQAIDAGDAYEITNILSDNQARWKTYGSGNALEFPDLDN
                     RPVAAKTGTSADWRDNWTVGYASDLVVGVWVGNTDNAPMEQIDGIAGAAPIFHDFMVA
                     AHSEEYQAVLADPNGDLTPVGFVRPDDVVNVEACVATGKLPVSGMPSYMEIASIDDVP
                     RTSCDQLTQREADELRAALVDVTVNGSRYTDEGLASLLRYSEAVGGGVGIPDDPIEPT
                     PTPTPTPTPTPTPTPTPTPTPRPTNTPTPTQPSVTPTRTGVSGPTTVIVPNLFGLTLN
                     QAVTEITSSGLTVGTIGYFDQSDLPPGIDITVVDVGEVLIQTPGPGISVQSGTAVSIA
                     IRAQ"
     gene            9386..9742
                     /locus_tag="VFV93_10470"
     CDS             9386..9742
                     /locus_tag="VFV93_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5165809.1"
                     /translation="MVSNWAIIGAMILTATVMGSSLILLGKLFRPNNPTPLKEAPYES
                     GIPMVDPPRARYTPRYYVVALLFVVFDIEAIFLFPWAVAFDALGLFGYIQAVVFICLL
                     LAGLAYEWKKGALEWV"
     gene            9733..10356
                     /locus_tag="VFV93_10475"
     CDS             9733..10356
                     /locus_tag="VFV93_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5165810.1"
                     /translation="MGLIDNRFEKNVVTTSVDWVFNWARRSSLWWLQFGLACCAIEMI
                     AASMPRFDLSERFGMLYRATPRQADLMIVAGTVTKKMAPVVRTLYEQMAEPKWVLSMG
                     SCANVGGPFDTYAVVQGVDQVVPVDCYVPGCPPIPEGLYYGILELQNKVIKYETFAKK
                     YGHDEAEAERERDRIAARQTIAGTDAMIVSQTAGFGEPSDNSFPRAS"
     gene            10438..>10740
                     /locus_tag="VFV93_10480"
     CDS             10438..>10740
                     /locus_tag="VFV93_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165811.1"
                     /translation="MSQDPARKDPNTPAEAVATGAESTTAGPKDDPASTPTTDNQRMQ
                     AEASPAGDVSNADDPDTSDARAALAQMSDPSMPVIEPAHDMTGVQVIDPVWVHSGYW"
BASE COUNT         1918 a         3603 c         3413 g         1806 t
ORIGIN      
        1 ggctggctcg gccctgaggc gcttctgccg cgggcaggga ctggcagtga tggcagccgc
       61 acactggcgt ccgtcgagtt gacggaggtg gatctggacc gactgtcgat tgatgcccgc
      121 tcgacggcct ccggcggcga ctacttcgtc gccagcgagc gtgggagcgg ggttccgttg
      181 atctggaacg tcgacgggaa ccgcccgcga cggctgacca gcgacatcac cgacctggcc
      241 tggttcaacc acgggacggc cgccatcggc gtcgccgcgg gtcaggcggg cgacagcggc
      301 agccggatcg tcgtcacagc cgctgatatc gccagtacga ccgcctacct cgatcaccag
      361 tacttcgacc ccgccaacct cggttcctcg gccgaccagc gctacgcccg gccgatggtc
      421 tcgccggccg gggcgctcgt ctcgttcttc gtcgtcgatc gcctcagcgg cgacgtatcg
      481 ctctggctgg acaccggcga gggggccgcc gaagtggtcg ccggctggac gcttccggtc
      541 cagcgcgcgc tcgatccgcc gctcgtcgcg acctgggcga gcccagacac actcctgttc
      601 gcgatgccgg ctgactggca cgacgggctg cctcagacga ccgcctttat gcgcttgacg
      661 ctctcgtcgg atacaccgca gttggacgaa ctcgcacgga tcgatgccag ccgtggcgcg
      721 aaggggatcg tgctcgccga gctggcgctc agccccgacg ggtcgctgct cgcgtatcga
      781 ctgcgccagt tcgacagcgt gtcgggtagc aatgcaactg aggacacact ccacgttgcc
      841 ggcaccgaca acctcaacca ggcgatcgaa cttgagcgcg gcggcagcag cgacgggttg
      901 ctctggacga cggccggcga tcagctcgtg gccggcatcc gggggcggat cgcgctctat
      961 tccgcggatg gacgcgacct cgcctacctc agcccgcgcg gcgtccacgc gtcctacccg
     1021 ctcctgatcg gccgccaggt ctggttcgag gccgtcgatg acaggggcga acgtgtctgg
     1081 caggtggatc tggaggagtg actccggaaa cctcacgtcg ttgcctgaat ctcccgccgg
     1141 tcccatactg cgcgtcaaca tcagtttggc gtcgcgaaag gggagtctgg tatggcacga
     1201 ccgctcaaag tgggattcat cctgccgcag atcgagggcc ggcgcagcgg cacgacgccg
     1261 cgctggtcgg agatccgcga gatggccgaa gtcagcgagc agatgggctt cgactcgctc
     1321 tgggtggtcg atcacctgct ctacggcttc ggcaaggacg atccggtccg cggcgtctgg
     1381 gagggctggt cactgttggc ggcgctggcg gccgtgacga ccaggccgga gctgggtacg
     1441 ctggtcgtct gcaccggctt ccgcagtcca gcgctgctgg cgaagatggc cgacaccgtc
     1501 gatgagatca gcgacggacg gctgatcctc gggatcggcg ccggctactt cgagcgtgag
     1561 tacaacgcgt tcggcatccc ctacgaccat cgtttcagcc gcttcgagga ggcgctggag
     1621 atcatcgccg gcctgctgaa acatggcgag atcgactatg aggggcagta ctactttgcc
     1681 cgagaaagtg agctgcgccc acgtggcccg cgcccgggtg ggccgccgat catggtcggc
     1741 acgcggggtg agaagatgct gcggctgacg gcgaagcacg ccgacgcctg gaacggctgg
     1801 ctggcgcgcg gcgacaatcg cccagccgcg attccgccgc tccgcgccga cgtcgacgcc
     1861 gcctgcgtcg aggtcggccg tgaccccgct acgctcgacc ggacggcgac cgtcttcgtc
     1921 gatcccaccg atcgagccgc ccagtcgagc cgggggccgg tcagcggctc ccctgaggag
     1981 atcgcggcga cgctggcggc cttcgccgat gagggcatca cccacatcca gatcgcctcc
     2041 aaagtcaccc gagtccagga gttggagcca ttcgcgtcag tgttggaagc attggaccgg
     2101 ctgtagcctg gttcacaatc agcccaatgg tcaatccggt acgggcgata tcgcaatatc
     2161 gcccttcttg atgcccggcg cgtggaaaag ggcgagatcg caatatcgcc cgtaccggat
     2221 tgccactgga ctgattgtga ggtgcggggc gctgccgccc tgcgccgagg tgcgcgtttg
     2281 tgtagcatat gtcggatgaa aactgtgaac gtaaacagac tgatacggtt gctcgttgtt
     2341 gtcctgctgg cagccggagt tgtcgcctgc agcggaccgg acgacagcga ctccgaaacg
     2401 gcgaccagcg ccgtcgagca gggtggttcg tcagggaccg cagccggcca gccgaccgag
     2461 acgagcggcg acgtcccggc tgagacgcca tcagcaacga ccgacccggt ccatacggct
     2521 ggatcgacga cggacgcaca accgaccgca acgactgacc cggttccacc aacgacggcc
     2581 gcgccgaccg aggcggttgc cccgacgccg accgcgccgg tggctcaggc cggtgggctg
     2641 ctgcccaaca accgcatcct ctcgtactac gggcatccga actcggccgc gatgggcatc
     2701 ctcggtgagt acacgatgga agacgcccac gcgcacctgg ccgagcaggc ggcagaatac
     2761 gaggccgccg atcctgccca ccccgtcacg atcgcattcg agctgatcgc gaccgtcgcc
     2821 cagccgtggc ccggtgatga cggtacctac gtcgcctaca ccggcgacga gatcattcag
     2881 gagtacgtcg actacgtcac tgccaataac atgatcctga ttctggacct gcagatcggt
     2941 ctcgacacga tccccaacca gatcaacatg atccgtcact ggctggagta tcccaacgtc
     3001 cacgtcgcgc tcgatcctga gttctcgatg aagggcaatg agatcgtccc gaacgaccgc
     3061 atccccggcg aattcatcgg cgaggcgaac ggcatccaca ttcaggaggg gatgaatctc
     3121 gtctcgcaga tcgtcgctga ggccgggatt ccgccgaagc tgttcatcgt ccatcagttc
     3181 gaagaggaca tgatcttcaa taaggaccag atcacgccgg tcgctggtgt ccagttcgtg
     3241 ctggacatgg acggcttcgg cggcagtgac gccaagctgg ggaactacac ccacttcgtc
     3301 cacgatcagt tgatcgagtt cggcggcatc aagctgttct acaaacagga cgatccgctg
     3361 cttaccccgc ccgagatcgt cgccctcgat ccaccggcac tggttgtgat ctaccagtag
     3421 cgcgaatcac gaccagacca aattatcatg aggagcaagc tgtggaactt tccgaacggg
     3481 ctgcggcgtt gcaccagcgg gcgatcgtga tcgacggaca cagcgacatc ctgctgccgg
     3541 tcgtgatggg gacgacgacg ctgacgacgc cgtttccgga gtccgagctg gaacgctggc
     3601 agcgtttggt ggaagtccaa cagccggccc ggcgcagcag cgacgtgccg tatgggctcg
     3661 atccactcgc gatagcgatc gcgccatccg ggcagtacga gctgccgctg ctggaagcag
     3721 gcggggtcac ggccgaatgc gtcgctctct tcctgcctga gacgatgctc gacaacgcgc
     3781 tcgaatcggc gttggagatg gtggctgccc tccatcgcga gatcgaagcg aacgctgacc
     3841 gctgcctgct ggtccgttcg gtggcggatc tccagcgtgc gaaggccgag ggcaaggtcg
     3901 cctacatcct gacgatggag ggcgccgaac cgatcggcac gcgcatcgat ctgctcgaca
     3961 tcttctggcg gcttggtctt cggatgacaa cgctgaccca cagtcgccgc aacctgctgg
     4021 ccgatggcac ccagcaggac atcaacaccg gcgggctgac gagggccggt cgcgaggctg
     4081 tctgccgttg ccaggaactg gggatcgtca tcgacacggc ccacatctcg gaccgaggct
     4141 tctgggacct gatcgaactg gtcgatggcc cgatcctctg ctcgcacacc agcgtcctga
     4201 cattgacgcc cggctaccgt gccccgtggg acgagatcaa cccgacctac gggatgtcca
     4261 aggccgaagc catcgcccgc aagggcggtc tgatcggcgt cgtgttctgg agcatggccg
     4321 acacggccgc gctggtacgc gaggtcgagc ggatcaccga gctgaccggg ccggaccatg
     4381 tcggcctcgg caccgacttc ttcggcttcc gcgatgcgcc gagcgatctc cagcacatcg
     4441 gccaacttcc cgccctgacc gaagcactcg tccaggccgg cctcgacgac gacacgatcc
     4501 tcaaggtcct gggcggcaac tacctgcgca tcttcgagca ggcctggcga gactagcgca
     4561 aggcgattca ctcaacccaa tcaatcccca atccagtacg ggcgatatcg caatatcgcc
     4621 ctgaatccct gccgaggcgt tcgggcggga gagccgtgcg atattgcgat atcgccccta
     4681 cgcgattggg attgggattg agcggtttcc gacgtgacat cgtacagatt cagcgatcgc
     4741 cgctgaactg tttcctgatc tcgtcgcgaa acacctcggc acgtggcgcc agaccggtgg
     4801 cacctgaccc ccgcacgacg aacacgaccc gctgaaggct ggaaccggca agaagatgtt
     4861 cgcggacggc ggccaccatc gctttcgcgc aggcgtcgat gtcgaggccg ccgactccgg
     4921 tgccgagtgc ggggaaggcg acgctgtcta gtgcctgctc gtcgcagagc tgcaacgcgc
     4981 ggcgggttgc ggcagcgact gtttcggtcg aagcatagat ctgagtccgg tcggtgaagc
     5041 ccatcgctgc cgcgtgaatg atcgcgcggg ccggcggcgg catcttcccg gcgctggtga
     5101 tgacggcctc gccaaccgcg atcggtccct gacgaacggc ctcctgctcg acctcggcgc
     5161 cggctgcccg tttgagcgcc ccggccacgc cggctcccat ccagagctcg ttgttggcgg
     5221 cattgacgta cgcctcgcag ccgatggccg cgacatcggc gatatctgcc tcaataactt
     5281 ccacccgtat agccttctta tcgtccatgg ccaccatcca atcccgcgtc ccgtccggcc
     5341 cggcgtcagg atggccggcg ggcgtcacca tgtggctaga ttctggcaga aaggatcgca
     5401 aatcggtgac cctctggaag tcttccgccc tgatcgcgtt cgtgctcgtc ctgctgctgg
     5461 ccggctgcgg cggcgacggt cgacccgacc cgaccagcac ggttaagacg acggccgagc
     5521 cgggcgaccc gcacgtgcta gagccgatga cgaccggccg cgatatcgtc gcgcccgacg
     5581 gccgttactc gttgcggata cccggcgagt gggtggagta tgacgatccg ttcgcggaac
     5641 tatcgttccg aaccgttgct gaggaccctg cgctggcgat caacgtcaac cgcgatgaga
     5701 ttgacgagag cacccgcgtc caggtctatg cagaagaggc ccgcgcacga atctcgcgaa
     5761 cgtatcgcaa cgtcaactcg ctctcactga ctccagtgaa gatcggctca atggaggcct
     5821 accgctggat ctacaccgct gctgtcggca cgatcgagca tctcttctat caggtgttcc
     5881 tcatcgacgc cggtgagggc ttcgtcctga ctggttccgc cccgatcgat gttgatatcg
     5941 acctcgtccg ccccatcttc gacggcatcg ccggctcaat gaccttcgca cggggctgaa
     6001 cggggggccg tcaggaggcg tcctcgatct ggcgtgccag ttcgagatcc aggttcgtga
     6061 tgccaccggc ctcgtgagtc gtcaggaaga gaatcaccgt tcgccagcgg atgtcgatgt
     6121 cggggtgatg accagccgct tcagccaggt cggcgatctg gttgacgaac gcgatcgcgt
     6181 cgcggaagct ggcacgccga tagcgcttgc ggatgccgcc ctcttcgcgg cgccagcccg
     6241 gcaggctgcc gagtgcgcgc tcgacccctg cattgctgat cggttgagcc ggttggtcct
     6301 gttcctcgtc cgtattcatc gtgactcctc ctgcctgccg gcgtttccat gtacaactgt
     6361 atccacacga gccggaaacg ctccagcctg atgcatggtc cggccagatg atcgcggagg
     6421 acgatgtcgt cacgtcgccg ctacatctca cgagctaacc gcctgcgctc gactcgacgc
     6481 gggcagtccg gctatggcgc gcggccggga tcgcctgccc ggcgcgtctc gccgcgcctg
     6541 ctggcgaccc acgatctgcg ccagaggcgg gaccagggcg agcgagttac ctcgatggcc
     6601 cgcatcgcca gcgtcgccgc gatctttgcc gcgacgctgt tcgtcggctt cgcgatcgcc
     6661 agcgcgagtg cggccgtcgc ggcctggaac tacttcagcg aggatctgcc atcgattgcc
     6721 gacttcgagg cgcgtcagtt cgagacgacc cgcatctacg accgcaacat gaatctgctc
     6781 tacgaagtca gcgacccgga gaccggctgg cgcacgtatg ttccgctacg ggagatcacc
     6841 gccaacgaca ccaacacctt catcgtcgac gcgacggtcg ccgccgaaga cgagaccttc
     6901 tggaagaact atggcgtcga accggccgcc atcctgcgcg gcgcgttcat caatgtctcc
     6961 ggccgtggct cgtcgggtgg atcgacgatt acccagcagc tcgttcggca gctgtatccg
     7021 gagaagatcg gcttcgagcg ctcgtatgac cgcaagatcc gcgaggcgat cgtcgccttc
     7081 cagttcaccc agcactactc caaagagcag attctcgaga tgtacctgaa ctcgatcttc
     7141 tacggcaacc gcgcctacgg gatcgaggca gccgcccagg cgtacttcaa caagcacccg
     7201 tccgagttga cgctggccga ggcgtcgatg ctggccggct tgccacaggc gcccagctac
     7261 tacgatccga ccgtcaacat ggaggctgcc aagacccgcc agcgctacgt gctggatcgg
     7321 atggcccacg atggttacat cacccagcaa caggctgacg cggcctgggc cgagatcctc
     7381 atcccgcagg cgcgcgacag tcaactcaat ccagcgccgc actgggtcaa cttcgtcatc
     7441 gatcatctgg aggcacgcta cggagccgag aaggtctatc gcggcggttt gacggtccgg
     7501 acgacgctcg atctgggact ccagcttgag gccgaagccg ccgttcgcaa gcatctcgac
     7561 acgctgggac cctacaacgc gaccaacggc gcgctggtcg cgctcctacc gggaacgggt
     7621 gaagtcgtcg cgatggtcgg ttcgcgcgat tacagcgacg acacgatctc aggccaggtc
     7681 aacgtcgccg tctccgagcg gcagccaggg tctgccttca tgccaatcac ctacgcggcc
     7741 gccttcgagc agggttggta tcccggcacg acgattctcg attatcagac acaatggcag
     7801 acgcccggcg cgcccgagcc gcagtacgtg ccggagaata cgtcccgtac ctacaacggc
     7861 gcagtcactt cccggcaggc gttggcgggg tcgctgaaca ttccggcggt gaaggcgatc
     7921 gactacgccg gcgtggagaa cacgattgac ctggcccatc ggatcggcat tcgcgagggg
     7981 ctctggcgtg gggtcggtgt ctatgggctg gcgctgacgc tgggcggcgg cgaggtctcg
     8041 ttgctggagt tgaccaactc ctacgcgacc ttcgccaaca ccggccgtta cgtcggctac
     8101 aacccggtcc tggagattac cggtccggac ggggagacga tcgaccgcgc cgaccccggc
     8161 agcgcgctgc cgaacgcccc gcaggcgatc gacgccggcg atgcctacga gatcaccaac
     8221 atcctcagcg acaaccaggc ccgctggaag acctatggct ccggaaacgc gctcgaattt
     8281 ccggacctcg acaaccggcc cgtcgcggct aagaccggca ccagcgccga ctggcgggac
     8341 aactggacgg tcggctacgc gagcgatctg gtcgtcggcg tctgggtcgg caataccgac
     8401 aacgcgccga tggagcagat cgacgggatc gccggggccg cgccgatctt ccacgatttc
     8461 atggtggccg cccactccga ggagtatcag gcggtgctgg ccgatccaaa cggcgatctc
     8521 accccggtcg gtttcgtacg gcccgacgat gtcgtcaacg tcgaggcgtg tgtcgcgacc
     8581 ggcaagctgc cggtcagcgg gatgccctcc tacatggaga tcgcctcgat cgacgatgtc
     8641 ccgcgtacca gttgcgatca gcttacccag cgggaggccg acgagctgcg ggccgctctg
     8701 gttgatgtga cggtgaacgg cagtcgctac accgacgaag ggctcgcgtc actgctgcgc
     8761 tattcggagg cagttggcgg cggtgtcggc attcccgatg acccgatcga gccgacacca
     8821 acgccgacac caacgccaac tccgacgcca acacccaccc cgacgccgac accgacgccg
     8881 cgtccgacca acacgccgac gccgacccag ccgtcagtga cgccgacacg caccggcgtg
     8941 agcgggccga caaccgtgat cgtgccgaac ctgttcgggc tgacgctgaa ccaggcggtc
     9001 accgagatca catcgagcgg cctgaccgtc ggcacgatcg gctacttcga ccagagcgac
     9061 ctgccacccg gcatcgacat cacggtcgtt gacgtcggcg aggtcctgat ccagacgccc
     9121 ggccccggca tctccgtcca gtccggcact gctgtctcga tcgcgatccg ggcgcagtag
     9181 aggaaccttt cccgcaaccg ccaccctctg ggtcgcggtt gcgggaaggg ttttcgacgg
     9241 cacgctatgt gctgcgacag cgccgtacgc gctgcgtccg tacagtcgag gcgtgcctga
     9301 agcggctggg agatcgtgat atagtacaca agtcgggggc ggagccagta cagggcttcg
     9361 cgacgcggaa aggacggcgc ggcgagtggt ttcaaactgg gcaatcatcg gtgcgatgat
     9421 cctcacggcg acggtgatgg ggtcgtcgct gatcctgctc ggcaagctat tccgacccaa
     9481 caacccgacc ccgctcaagg aagcgcccta cgaatcgggc atcccgatgg tcgatccgcc
     9541 acgcgcgcgc tatacccccc gctactacgt cgtcgcgctg ctattcgtcg tcttcgatat
     9601 tgaagcgatt ttcctcttcc cctgggccgt cgcgttcgac gctctcgggt tgtttggata
     9661 cattcaggcc gtcgtcttca tctgcctcct gctcgccggc ctggcctacg agtggaaaaa
     9721 gggagcgctg gaatgggtct aatcgacaac cgctttgaaa agaatgtcgt cacgacctcg
     9781 gttgactggg tcttcaactg ggcacgccgg tcgagcctct ggtggctgca gttcggcctc
     9841 gcctgctgcg cgatcgagat gatcgccgcc tcgatgcccc gcttcgacct ctccgagcgc
     9901 ttcgggatgc tctaccgggc gacgccgcgc caggccgacc tgatgatcgt cgccgggacg
     9961 gtgacgaaga agatggcgcc ggtcgttcgc acgctctacg agcagatggc cgagccgaag
    10021 tgggtcctct cgatgggctc ctgcgccaac gtcggcggcc cattcgacac ctacgccgtc
    10081 gttcagggcg tcgaccaggt cgtgccggtt gactgctacg tcccgggctg cccgccgatc
    10141 cccgagggtc tctactacgg catcctcgaa ctgcagaaca aggtcatcaa gtacgagacg
    10201 ttcgccaaga agtatggcca cgatgaggcc gaagccgaac gcgagcgcga ccggatcgct
    10261 gcccggcaaa cgatcgccgg caccgacgcg atgatcgtca gccagacagc cggcttcggc
    10321 gagccgagcg acaacagctt cccgcgcgcg tcgtagcgca cgggtccatg cgcggcgcgg
    10381 gtcgcgcaac cggagggcgc ctgacgcccg tcagagccag caggaggcgg attcggcatg
    10441 tcccaggacc ccgcccgcaa agaccccaac acgccggccg aggccgtcgc gaccggtgcg
    10501 gaatcgacca ccgccggccc aaaggacgat ccggcaagca cgccaacgac cgataaccag
    10561 cgcatgcagg ctgaagcgtc gccggccggc gatgtctcca acgccgacga ccccgataca
    10621 agcgacgccc gcgccgcgct ggcgcagatg agcgacccgt cgatgccggt catcgagccg
    10681 gctcacgaca tgaccggcgt gcaggtcatc gacccggtct gggttcactc aggctactgg
//
LOCUS       DASUGJ010000471         6947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1603783, whole genome shotgun sequence.
ACCESSION   DASUGJ010000471 DASUGJ010000000
VERSION     DASUGJ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603783"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1867)
                     /locus_tag="VFV93_10485"
     CDS             complement(2..1867)
                     /locus_tag="VFV93_10485"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5165812.1"
                     /translation="MTVPTDQWHAIAQQIRLDSIRTSSKAKSGHPSSAMSAADVMTAL
                     MAKYLRYDFDNPENPNNDHLIFSKGHASGLLYGMYRAAGAISDEEMMTYRELGSMMQG
                     HPTPQIPWVDVATGSLGQGLPIGVGVALAGKYLDKLPYRVWVLCGDSEWAEGSIYEAL
                     DKAGHYQLDNLTVVLDMNRLGQRGETAHGWEADVYADRVRAFGCYPIVIDGHDFGEID
                     QALQEAVDHTGSPVVIIARTMKGRGVSFMENEFGWHGKAIGPDHIEQATKELGGKTDL
                     TIEVHEPPAGTPASTGKAGTLALPSYDKGDKVATRQAYGDALKALGAARPDVVVTDAE
                     VSNSTMSEVFKFAYPDRFFEMFIAEQQMVAAAVGFSVRGYTPFASTFGAFMSRAYDFI
                     RMAAISRANIRLCGSHAGVSIGEDGPSQMALEDIAMMRAVHDSTVLYPSDPNQTAQLV
                     AAMADQPGISYLRTTREKTPVIYDSAETFPIGGSKVVRQSDDDKVTVVGAGITLHEAL
                     KAADELAGSGVHIRVIDLYSVKPVDYKTVHAAVTATDGKLVVVEDHWPQGGIGSAILE
                     CFATHTAGGSDRSTDFRMTHLAPSELPGSATPQEQLDTNGISARHIVEAVMGLIG"
     gene            2113..2805
                     /locus_tag="VFV93_10490"
     CDS             2113..2805
                     /locus_tag="VFV93_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX5165813.1"
                     /translation="MLTRFKNRADAGRVLAGMLSKYAAQPDAIVLALPRGGVPVAYEV
                     AVALGLPLDIFLVRKLGVPGHEELAMGAIASGGVRVLNDDVVGPLNIGPTTIERVAAR
                     EQQELERREAAYRDGRPRPNLRGRTVILIDDGLATGSTMLVAIRAIKQQQPARIVAAV
                     PTAAPSVCAEIGREADEIVCAMTPEPFYAVGLWYDDFSETTDDDVRRLLQLASRRSAV
                     GSAHAQRETEAH"
     gene            2850..4196
                     /locus_tag="VFV93_10495"
     CDS             2850..4196
                     /locus_tag="VFV93_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX5165814.1"
                     /translation="MTDGGAVIDAVRGSAKLLTGSTADLDRLVSMVGDTRFVLIGEAS
                     HGTDEFYRLRADLTKRLIDERGFTAVAVEADWPDAYRVNAYVCGVGEDRSAEEALSGF
                     RRFPAWMWRNEVVVEFANWLRARNANLPADSSPAGFYGLDLYSLNTSIEAVIDYLDRV
                     DPDAAFRARERYNCFEMFSRDADTYGYLTEVGAAEPCEDEVVEQLVDLQRQIADYARR
                     DGRVAEDAAFYAEQNARLVKNAEQYYRSMFRGRVSSWNLRDRHMVETLDNLVAYLDRH
                     GRLTKVVVWAHNSHLGDARATEMGDAGEWNVGQLMREAHGDDTLLIGFTTHTGTVTAA
                     SDWGGIAETKLVVPSLPDSYERLFHDSGIERFWLPLRDDVALADLLRRPWLERAIGVI
                     YRPETERLSHYFRARLSDQFDALIHIDETSALQPVERLAPLPEHELPATFPSDVPT"
     gene            complement(4212..4982)
                     /locus_tag="VFV93_10500"
     CDS             complement(4212..4982)
                     /locus_tag="VFV93_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5165815.1"
                     /translation="MATHDRTVRLAAISDLHYGKGSQGSLQSVFSEISDTADILVICG
                     DLTDYGLPEEALILAKDLNANVKIPIVAVLGNHDYESGRHLEICDILTGAGVTILDGD
                     SVELRGIGFAGVKGFAGGFGRGALGYWGEPAIKAFVQEALDEALKLETALARLRSEQR
                     IAILHYAPIRGTVEGEPPVIYPFLGCSRLEDPINRYPVMAVFHGHAHNGTHEGHTTSG
                     IPVYNVSHGLLKKIQPSGSGFHVMEIAVTPAADTVPVM"
     gene            5164..6018
                     /locus_tag="VFV93_10505"
     CDS             5164..6018
                     /locus_tag="VFV93_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165816.1"
                     /translation="MITTPEHEQDGSATATFYRDALALMVDSPISFLVGGAFALQRYT
                     GLARDTKDIDIFVRPSDVRRTLRQFARAGYATEVPFPHWLAKARCGEFFMDIIFSSGN
                     GLAVVDDEWFAGATDAEVMGMPVKLAPVEETVWSKAFIMERERFDGADVAHLLHATAD
                     TLDWQRLLRRFDGHWPVLLSHLVLFGYIYPSKRDLIPGWVMTRLIERLQSEDASSADP
                     ICRGTYLSRAQYLPDITDWGYRDARVHPEGPMSAEDADIWTTAAIDDEAVTISIASDA
                     VVSETSAA"
     gene            complement(6111..6299)
                     /locus_tag="VFV93_10510"
     CDS             complement(6111..6299)
                     /locus_tag="VFV93_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165817.1"
                     /translation="MERSREVIEARKHAHDDVDNPVPFDGDMPEPDTAREDLLNRPIG
                     GQSNPNARGGTAKRQKAG"
     gene            6485..>6947
                     /locus_tag="VFV93_10515"
     CDS             6485..>6947
                     /locus_tag="VFV93_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX5165818.1"
                     /translation="MTSAKIPTPESVLGYRMGTARRLPDWGEIVNYFETLAAASDRVA
                     LERLGESTDGRPYIAVTVSSPENLARRDELRSMLERLHDPRDLPAEEAARLVEDGRVT
                     AFLLCTQHSNEIGAALMTLELAAELASKDDPETGEILDNVVTVMIPTHNPDG"
BASE COUNT         1209 a         2190 c         2258 g         1290 t
ORIGIN      
        1 attatccgat caagcccatg accgcttcaa cgatgtgccg tgcgctgatc ccgtttgtgt
       61 cgagctgttc ctgcggtgtt gccgagccgg gcaactccga cggcgccagg tgtgtcatcc
      121 ggaagtcggt cgagcggtcg gagccgccgg cggtatgggt cgcgaagcat tcgaggatcg
      181 ccgagccgat cccgccctgc ggccagtggt cctcgacgac aaccagcttt ccgtcggttg
      241 ccgtgacggc cgcgtgaact gtcttgtaat cgaccggctt gaccgaataa aggtcgataa
      301 cgcggatgtg gacaccgcta ccggccagct cgtcggccgc cttgagcgcc tcgtggagcg
      361 tgatgcctgc cccgacgact gtgaccttgt cgtcgtcaga ctggcggacg actttactgc
      421 cgccgatcgg gaacgtctcg gcgctgtcgt agatgactgg cgtcttctcg cgggttgtgc
      481 gcaggtaaga gataccgggc tggtcggcca tcgccgcgac aagctgggcg gtctggttcg
      541 gatcggacgg gtagaggacg gtgctgtcgt ggacggcccg catcatcgcg atgtcttcga
      601 gcgccatctg cgacgggccg tcttcgccga tcgagacgcc ggcgtgggag ccgcagagcc
      661 ggatgttggc gcgcgagatg gcggccatcc ggataaaatc gtaggcgcgg ctcatgaacg
      721 cgccgaaggt cgaggcaaac ggtgtgtagc cacggacgct gaacccaacg gcggcggcaa
      781 ccatctgctg ctcggcgatg aacatctcga agaagcggtc cgggtaggcg aacttgaaga
      841 cctcggacat ggtcgagttg gagacctcgg cgtcggtgac gacgacatcc ggccgggccg
      901 ccccgagtgc cttgagtgcg tcgccgtacg cctggcgggt agcgacctta tcgcccttgt
      961 cgtagctggg cagcgcaagc gtgccggcct tgccggtcga ggcgggcgtg ccggctggcg
     1021 gctcgtggac ctcgatcgtg aggtcggtct tgccgccaag ctccttggtg gcctgctcga
     1081 tgtggtccgg accgatcgcc ttaccgtgcc agccgaactc gttctccatg aacgagacgc
     1141 cgcgtccctt catcgtccgc gcaatgatga cgaccgggct accggtatgg tcgacggctt
     1201 cctgcagagc ctggtcgatc tcgccaaaat cgtgaccgtc gatgacgatc ggatagcagc
     1261 cgaaggcgcg gacgcggtcg gcgtagacat cagcttccca gccgtgggcc gtctcgccac
     1321 gctggccgag ccggttcatg tcgaggacga cggtcaggtt gtcgagctgg taatggccgg
     1381 ccttgtcgag tgcctcgtag atcgagccct cggcccactc cgagtcgcca cacagcaccc
     1441 agacgcggta cggcagcttg tcgagatact tgccggccag tgcgacgccg acgccgatcg
     1501 gcaagccctg tcccagcgat ccggtcgcga cgtcgaccca cgggatctga ggcgtcgggt
     1561 ggccctgcat catgctgccc agctcgcggt aggtcatcat ctcctcatcg ctgatcgcgc
     1621 cggctgcccg gtacatgccg tagagcaggc ccgacgcatg gcctttcgag aagatcagat
     1681 ggtcgttgtt cgggttctca gggttgtcga agtcatagcg cagatacttc gccatcaggg
     1741 cggtcatcac atcggcggcc gacatggccg agcttggatg gccggatttc gctttcgagc
     1801 tcgtgcggat gctgtccagt cggatctgct gcgcgatcgc gtgccattgg tcggttggaa
     1861 cagtcatgct cgggtccctc ttcctagctg tcagctctcg gccgtcacct gtcagccgaa
     1921 atcggctcga cggcggtcgc tatccgtcaa tcttacgccg tggttactgg caatcagcat
     1981 cgccgggacg ttcttgtcgc cgctggctga ctggcataga ttctgcaccg cttcaatgaa
     2041 ggcactgacg cgaggcgaac cagcgaaacg ctgcacattc cgcgccttgc atgatggatc
     2101 gggggccgga cgatgctgac gcgctttaag aatcgagccg atgccggtcg tgtgctggcc
     2161 ggaatgctct ccaaatatgc cgcgcagccg gacgcgatcg tgctggcgct gccccgtggc
     2221 ggggtgccgg tcgcctacga ggtcgccgtc gcattgggat tgccgctcga catcttcctg
     2281 gttcgtaagc tgggcgtgcc gggacacgag gagctggcga tgggcgcgat agcctccggc
     2341 ggtgtccggg tcctcaacga cgatgtcgtc gggcccctca acatcggtcc gacgacaatc
     2401 gagcgcgtcg ccgcacgcga acagcaggaa ctggagcggc gtgaagccgc ctaccgcgat
     2461 ggccggccaa ggccgaacct gcgcggccga acggtcatcc tcattgacga cggactggca
     2521 accggttcga cgatgctggt cgcgatccgc gcgatcaaac agcagcaacc ggcccgcatc
     2581 gtagcggcgg tgccgacagc ggcaccgtcg gtctgcgcag aaatcggccg ggaagccgac
     2641 gagatcgtct gcgcgatgac ccccgagccg ttctacgccg tcggcctctg gtacgacgac
     2701 ttctccgaga cgactgatga tgacgtgcgc cgtctactgc aacttgcctc ccgtcgctca
     2761 gccgtcggaa gcgcgcatgc acaacgggag actgaagcgc actgacgaac gtggcgtcga
     2821 tgcaggaagt gatggggaca atggaccgaa tgacagacgg aggcgcggtc attgatgccg
     2881 tgcgaggctc agccaagttg ctcaccggga gtaccgccga cctcgatcga ctcgtctcga
     2941 tggtaggtga tacgcgcttc gtgctgatcg gcgaggcgtc gcatggcacc gacgagttct
     3001 accgactacg ggccgacctg acgaagcgcc tgatcgacga gcgtgggttc acggccgttg
     3061 ccgtcgaggc cgactggccg gacgcatacc gtgtcaacgc ctacgtgtgc ggcgtaggcg
     3121 aggatcgcag cgccgaggag gcactttccg gattccggcg cttcccggcc tggatgtggc
     3181 gcaacgaggt cgtcgttgag ttcgccaact ggctgcgtgc gcgtaacgcc aacctgcccg
     3241 cggactcgtc tccggccggc ttctacgggc tggacctgta cagcctcaat acgtcgatcg
     3301 aggcagtcat cgactacctc gaccgtgttg atccggacgc ggccttccgg gcccgcgaac
     3361 gctacaactg cttcgagatg ttctcgcgtg atgccgacac ctacggctac ttgaccgagg
     3421 tcggtgcagc cgagccgtgt gaagatgaag tcgtcgagca actggtcgat cttcagcggc
     3481 agattgccga ctacgctcgc cgcgacggac gtgtagctga agatgcggca ttctacgccg
     3541 agcagaacgc gcgcctggtc aagaacgccg agcagtacta ccgctcgatg tttcggggtc
     3601 gtgtctcgtc gtggaatctc cgcgaccggc acatggtcga gacactcgac aatctggttg
     3661 cctacctcga ccggcacggc cggctcacga aagtcgtcgt ctgggcgcac aactcgcatc
     3721 tcggcgacgc gcgggcaacc gagatgggtg acgccggcga atggaacgtc ggccaactga
     3781 tgcgcgaagc gcatggcgat gacacgctgc tgatcggctt caccacccac actggaacag
     3841 tgacagctgc cagcgactgg ggcggcattg ccgagacgaa gctggtcgtg ccctcgctac
     3901 cggactcata cgagcggctc ttccatgact ccggaatcga gcgcttctgg ctgccactgc
     3961 gcgacgatgt ggcgctcgct gatctcctgc gccgcccgtg gctcgaacgg gcgatcggcg
     4021 tgatctaccg gccagagacg gaacgtctca gccactactt ccgcgcgcga ctatcggacc
     4081 agttcgacgc gctgatccac atcgacgaga ccagcgccct ccagcccgtc gaacgcctgg
     4141 ctccgctgcc tgagcacgaa ctaccggcga cgttcccaag cgatgttccg acttgatcgc
     4201 cgtttccgga attacatgac cggcacggtg tcggcggccg gcgtgacagc gatctccatc
     4261 acatgaaagc cgcttccgga gggctgaatc ttcttcaaga gaccgtggga tacgttgtag
     4321 accgggatgc cgctggtggt gtggccctcg tgggtgccat tgtgggcgtg tccgtggaag
     4381 acggccatga cgggatagcg gttgatcggg tcttcgagcc ggctacaacc gaggaacggg
     4441 tagatgaccg gcggttcgcc ctcgaccgtg ccgcgaatcg gcgcgtagtg caggatggcg
     4501 atccgctgtt cagaccgaag ccgcgccagc gccgtttcca gcttcagcgc ttcgtccagc
     4561 gcctcctgga cgaacgcctt gatggccggc tcgccccagt agccgagcgc tccacgaccg
     4621 aagccgccgg caaagccctt gacaccggca aacccgatgc cgcgtagttc aacgctgtcg
     4681 ccgtccagga tcgtgacgcc agcgcccgtc aggatgtcgc aaatttcgag gtgccggcct
     4741 gattcgtagt cgtggttgcc gaggaccgcg acgatcggaa tcttgacgtt cgcgttcaga
     4801 tccttcgcca gaattagtgc ctcctcgggc aggccgtagt cggtcagatc accgcaaatc
     4861 accagaatgt cggcggtatc agagatctcc gaaaataccg attgcaacga tccttgggag
     4921 cctttgccgt agtgcaggtc gctgatcgcg gccagccgga cggttctgtc gtgcgtagcc
     4981 atgctgcccc ttcgtacgtt cgccggttga agatgccagc cggcgcggca tttgctgtcg
     5041 tatcggtgca ggcaagatgc ataccgcatg gtcgggtggc acgacccttg ccctatcacg
     5101 gcacgctcaa ctgcgggttg agcgcgggcc gatcgagaca gatgacccgc tgggagcaga
     5161 ccgatgatta cgacgccaga gcacgaacag gatggctccg cgacagcgac cttctaccgc
     5221 gacgcgctgg cgttgatggt tgattcaccg atctcgtttc tggttggcgg cgccttcgcg
     5281 ctgcaacgct acaccggcct ggcacgcgac acgaaggaca tcgatatctt cgtcagacct
     5341 tccgacgttc ggcggacgtt gcgacagttc gcgcgagcgg gctacgcgac cgaggtaccg
     5401 ttcccgcact ggctggccaa ggcccgctgc ggcgagttct tcatggacat catcttcagt
     5461 tcaggtaacg gcctcgccgt cgtcgatgac gagtggttcg ccggcgcgac ggacgccgag
     5521 gtgatgggga tgccggtcaa gctggcgcca gttgaagaga cggtctggtc gaaggcattc
     5581 atcatggaac gcgagcgctt cgatggtgcc gatgtcgcgc atctcctgca cgccacggca
     5641 gacacgctcg actggcagcg gctgcttcga cgcttcgacg gccactggcc agtccttctc
     5701 agccatctcg tcctgttcgg ctacatctac ccgtccaaac gcgacctgat accaggctgg
     5761 gtgatgactc ggttgatcga gcggcttcag tccgaagatg catcgtcggc agatccgatc
     5821 tgtcgaggca cctacctttc acgggcacag tacctgcccg atatcaccga ctggggctat
     5881 cgggacgccc gcgtccatcc agaaggccca atgtcagccg aagacgccga tatctggacg
     5941 acggccgcga tcgacgacga ggctgtgacg atctcgattg ccagcgacgc ggtcgtcagt
     6001 gagacatccg cagcctgatc tcgtcgctga cgcagccctc aatggccggt catccaggtg
     6061 gttaccccgt ccggggccat gttgaccccg atgcgccatg cgtggtgccg ctagccggcc
     6121 ttctggcgtt tggcagtccc gccgcgcgcg ttggggttgg attgcccgcc gattggtctg
     6181 tttagcagat cttcacgtgc ggtgtcaggt tccggcatgt cgccgtcgaa tggcacggga
     6241 ttgtcgacgt cgtcgtgcgc gtgcttacga gcctcgatga cctcacgtga gcgctccacg
     6301 ttgcgctcta tcgtctcgtc gtcgccgaag tagtgctgaa ttgcgcttgt caggcatcgt
     6361 ggcctcccaa cctcgatcga gttcaaacat tcagtcaggt cgcaggaaca acgccacgcc
     6421 ttccgggccg cgtatcatca gcggggagtt gaactgagga tggtgcagga agggaaacgc
     6481 tgtcgtgacg agcgcgaaga taccgacgcc ggagagcgtc ctcggctatc ggatggggac
     6541 ggcccgccgg ctgcccgact ggggcgagat cgtcaactac ttcgagacgc tggctgcagc
     6601 gtccgatcgc gtcgcgctgg agcgcctggg cgaatcgacc gacggccggc cctacatcgc
     6661 agtgacggtc tcgtcgccgg agaatctggc gcgtcgggac gagctgcgct cgatgctcga
     6721 acggctccac gatccgcgcg acctgccggc cgaggaagcg gcacgcctcg ttgaagacgg
     6781 ccgtgtcacc gccttcctgc tctgtaccca gcactcgaac gagatcggcg cggcgctgat
     6841 gacactggag ctagcggctg agctggcgag taaggacgac ccggagacgg gagagatcct
     6901 cgacaacgtc gtgacggtca tgatcccgac tcacaatccc gacggcc
//
LOCUS       DASUGJ010000472         4401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1324605, whole genome shotgun sequence.
ACCESSION   DASUGJ010000472 DASUGJ010000000
VERSION     DASUGJ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324605"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFV93_10520"
     CDS             <1..471
                     /locus_tag="VFV93_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HEX5165819.1"
                     /translation="INIFFPNSPLHDGAVIVRGNRLLAANCILPLSDREIPGGKIGTR
                     HQAAIGITEESDAIAVVVSEETNRISVAHSGRLTENLDSERLRRALRSLLKVTDRDAR
                     TGGGGSGDNGGRPFWMLRGSGGSSNGRARRGDGGPTPMKPAASSMPSEKSTPHD"
     gene            464..1756
                     /locus_tag="VFV93_10525"
     CDS             464..1756
                     /locus_tag="VFV93_10525"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX5165820.1"
                     /translation="MIDRLKRFADTGNAVRFFLSFILAFALWAWVTNERDPEQTYRAS
                     QVPVSAVGIPENLEIVGAVPAVDVTLQGPRSVIQTIDAASLTAEVNLDDVDGPGSYDR
                     KVHVNAPDGIRTITTDPETITVELDTVVSKTFPVEALPPAEIPRSLTVTSISVEPQQV
                     TVTGVQSNVDQVARVLAPINIGDNTDSFSDLISLSAVDQNNVEVENVEIEPGAVRVRV
                     DLEVRGKEIPVFVQCECDDVAEGFVVVGQPQANPANVIIDGDPEVLAQVPYIYTTPVN
                     TSGLDQTTVIDGVPLDIASLPDGVTVDPQFVDVSVRVEQTQFTRTLENIPIEVLGTEP
                     NTRVVVNPATLSIDISGPRDKLDQLEPRDVAVVVDVTGLDVGAHQVRPRVALPAGMTY
                     SDALPQVVVAIIDTSPTATSTARPAPTSAPEPTPTPNP"
     gene            complement(1770..2381)
                     /locus_tag="VFV93_10530"
     CDS             complement(1770..2381)
                     /locus_tag="VFV93_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5165821.1"
                     /translation="MSTMTPTRRARIIIADDESLIRMDLREMLGHLGYEVIAEASDGR
                     GAVELAKKLLPDLIIMDIKMPDMDGIAAAGELARDHIAPVVLLTAYSENSLIGRAKDA
                     GVCGYLVKPFRETELMPVIELALARFNELRELEREVTDLKDALETRKLVERAKGVLME
                     VHGLREADAFNRMRKTSMDNRKSMREVAEAILLTHEVQTGNSR"
     gene            complement(2521..2594)
                     /locus_tag="VFV93_10535"
     tRNA            complement(2521..2594)
                     /locus_tag="VFV93_10535"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2558..2560),aa:Pro,seq:ggg)
     gene            complement(2695..3081)
                     /gene="rplL"
                     /locus_tag="VFV93_10540"
     CDS             complement(2695..3081)
                     /gene="rplL"
                     /locus_tag="VFV93_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX5165822.1"
                     /translation="MAVNMDELIESIEQMSVLELSQLVKALEDRFGVTAAAPMMAAAA
                     PAAGGGATAEAAEEKTEFDVILTDVGPNKVQVIKAVRELTSLGLKEAKDVVEAAPKAV
                     REAVTKDEAEAAKAKLEEAGAKAQVQ"
     gene            complement(3158..3706)
                     /gene="rplJ"
                     /locus_tag="VFV93_10545"
     CDS             complement(3158..3706)
                     /gene="rplJ"
                     /locus_tag="VFV93_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX5165823.1"
                     /translation="MPTPKKVETVAELSDVLSRSKLTIVADYRGLTVADLQNLRGQLR
                     QHNAEMRVAKNTLTLRAAQQNNMEGLESALKGPTALVTAFDDPVQPAKVVSDFARSSR
                     ILAIRAAMLEGSLIGTAEIEALASLPSKDVLVAKVVGGLSSPLYGIVGVLAAPIRALQ
                     YVLQARAEQLGGSDAGEEGAAA"
     gene            complement(3909..>4401)
                     /locus_tag="VFV93_10550"
     CDS             complement(3909..>4401)
                     /locus_tag="VFV93_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX5165824.1"
                     /translation="AVGDAARIAQESGADYVGSDDLVKQINDGWLEFDATVAMADQMG
                     KVGGLGRILGRRGLMPNPRSGTVVRTPEDLPDVLNELKGGRVEFRNDRTGLLHVAIGK
                     VSFDEQRILQNLNALVDAVQRNKPSAQKGVYLRSITLTSTMTPGIPLDVSAAVAASSQ
                     AAA"
BASE COUNT          783 a         1474 c         1337 g          807 t
ORIGIN      
        1 atcaatatct tctttccgaa ctcgccgctg catgacggcg ccgttatcgt ccgcggcaat
       61 cggctgctgg ccgccaactg tatcctgcca ctgagcgacc gcgagatccc cggcggcaag
      121 atcgggacac ggcaccaggc cgcgatcggc atcaccgagg aatcggacgc gatcgccgtt
      181 gtcgtctccg aggagacgaa tcggatctcg gtcgcgcaca gcggacgact gacggagaat
      241 ctcgactccg agcgcttgcg ccgcgcgtta cgcagcctgt tgaaagtcac cgaccgcgac
      301 gcccgcaccg gaggtggcgg ctcaggagac aacggcggcc ggccattctg gatgttgcgc
      361 ggttctggtg gaagctcgaa tggccgcgcc cgacgtggtg acggcgggcc gacaccaatg
      421 aagccggcag cgtcgtcgat gccgtcagag aagtcgacgc cgcatgattg atcgcctcaa
      481 gcgcttcgcc gacaccggca acgccgttcg cttcttcctc tcgttcatcc tcgcgttcgc
      541 gctctgggca tgggtcacca acgaacggga ccccgagcag acctaccgtg ccagccaggt
      601 gccggttagc gccgtcggca tccccgagaa cctggagatc gtcggcgcag tcccggccgt
      661 cgacgtgacg ttacagggac cacgtagcgt gattcagacg atcgacgcgg catcactgac
      721 ggcggaggtc aacctggacg acgtcgacgg ccccggatcg tatgaccgca aagtccatgt
      781 caatgcgccg gatggcatca ggacgatcac gactgatccg gaaacgatca cggtcgagct
      841 cgacacggtc gtctcgaaga cgttcccggt cgaggcgctg ccgccggctg agattccacg
      901 cagcctgacg gtgacgagca tttcggtcga gccacagcaa gtaacagtca ccggcgttca
      961 gtcgaacgtc gatcaggtcg cgcgggtgct tgcgccgatc aacatcggtg acaacactga
     1021 ctccttctca gatctcatca gcttgtccgc cgttgaccag aacaacgtcg aggtcgagaa
     1081 cgtcgagatc gagcccggcg ctgtccgtgt ccgggtcgat ctcgaagtgc gcggcaagga
     1141 gataccggtc ttcgtacaat gcgagtgcga cgatgttgcc gaaggtttcg tcgtcgtcgg
     1201 ccagccgcag gcgaatccag ccaacgtcat tatcgacggc gatcccgagg tgctcgctca
     1261 ggttccgtac atctacacga cgcccgtcaa caccagcgga ctcgaccaga cgaccgtcat
     1321 cgacggcgta ccgctcgaca tcgccagcct gccggatggc gtgacggtcg atccacagtt
     1381 cgtcgatgtg tctgtgcgcg tcgagcagac ccaattcaca cgcacgttgg agaacatccc
     1441 gattgaagtt ctgggaaccg agccgaatac gcgggtggtc gtcaatccgg cgacactcag
     1501 catcgatatc tcggggccgc gcgacaagtt ggaccaactt gaaccccgcg acgttgccgt
     1561 cgtcgtcgac gtcaccgggc tggatgtcgg agcgcatcaa gtgcgaccga gagtcgcgct
     1621 cccggccggc atgacctaca gcgacgcgct cccgcaggtc gtcgttgcca tcatcgacac
     1681 ttctccaacg gcaacgtcga cagccaggcc ggctccaacc agtgctccgg agccgactcc
     1741 gacaccgaat ccatgatgtt cttgagtacc tagcgggagt tgccggtctg gacctcgtga
     1801 gtcaggagga ttgcctcagc gacctcgcgc atcgacttgc ggttgtccat gctggtcttc
     1861 cgcatgcggt tgaaagcatc cgcctcacgc aggccgtgaa cttccatcag gacgcccttg
     1921 gcgcgctcga ccagcttgcg cgtctcgagt gcgtccttca ggtcggtcac ctcgcgttcg
     1981 agctcacgta gctcgttgaa gcgggccagc gccagctcga tgacgggcat gagctcggtc
     2041 tcgcggaacg gcttgacgag gtaaccacag acgccggcat ccttggcgcg gccgatcagc
     2101 gagttctcgg agtaggccgt cagcaggacg accggcgcga tgtgatcgcg ggccagctcg
     2161 ccagcggcgg cgatgccgtc catgtccggc atcttgatgt ccatgatgat gagatctggg
     2221 aggagcttct tggcgagttc gacggcgcca cgtccgtcgg acgcttccgc gatgacctcg
     2281 tagccgagat gcccgagcat ctcgcgcagg tccatccgga tcagggattc atcgtcggcg
     2341 atgatgatgc gcgcgcgtcg ggttggagtc atcgttgaca taccacagcc ccattgtttc
     2401 ggtcgtcatg acatcgatcc gcctaccaca accgtgctcc attgcacgtc acggcgagca
     2461 ctgtcatcgc ccacgcggaa tccggcaatt cgcgaggctt ccagagttgg gtgggatcgg
     2521 tcggggcgag aggattcgaa cctccgacca ccggtacccc ataccggtgc gctaccggac
     2581 tgcgccacgc cccgtacggt tcagtatagc atacagacgg ggccgcactg cccgtgcgcc
     2641 ccgtctgtat gccggcaccg cgacgcgctg gaatgcgcgt caaatgcgcg aaaactactg
     2701 gacctgggcc ttggcgccgg cctcttcgag cttcgccttg gcagcctcgg cctcgtcctt
     2761 ggtaacagcc tcgcgaacgg ccttcggtgc cgcctccacg acgtccttgg cctccttgag
     2821 gccgaggctg gtcagttcgc ggaccgcctt gatgacctgg accttgttcg ggccgacatc
     2881 cgtcaggatg acatcgaact cggtcttctc ctcggcagcc tcggccgtcg cgccgccacc
     2941 ggccgccgga gccgccgctg ccatcatcgg cgcagccgcc gtcacgccga agcgatcctc
     3001 aagcgccttg acgagctgcg agagctccag gacagacatc tgctcgattg actcaataag
     3061 ttcgtccatg ttgaccgcca tcggggccgt ctccttctca tcttttcggg ttcaaaccgg
     3121 cgcgttcacc ggcaacgtcg atcgttgtgc tgatctccta agcggctgcg ccttcctcgc
     3181 cggcgtcgct gccaccaagc tgctcggctc gcgcctgcaa gacgtactgg agcgcacgaa
     3241 tcggcgccgc caggacgcca acgatgccgt agagcgggct ggacagaccg ccaacgacct
     3301 tggcgaccag gacatcctta ctcggcaggc ttgccagcgc ttcgatctcg gccgtgccga
     3361 tcagcgaacc ttccagcatc gccgcccgaa tcgccaggat tcgggacgat cgcgcgaagt
     3421 ccgaaacgac cttggccggc tggaccggat cgtcaaaggc agttaccagc gccgtcggac
     3481 ccttgagggc cgattcgagc ccctccatgt tgttctgctg ggctgcgcgc agcgtcagcg
     3541 tgttcttggc gactcgcatc tccgcgttgt gctgccggag ctgcccgcgc aggttctgca
     3601 ggtccgcgac agtcaggcca cggtagtcgg cgacgatcgt gagcttcgat cgactcagca
     3661 cgtcagacag ttccgcgacg gtctcgacct tctttggtgt cggcattccc attcacctcc
     3721 cctctgccac tcaaacttcc tcgacaacga acaacgcccc cgcgccacga gggcacgggg
     3781 gcgtgacatg tcacacctgt tagccgggtt gaaccagctc tcccgctacc tcggccggat
     3841 gattaagcaa cgtgacgttg ccccggctgt cttcggcgcg tcgtgattcg tctgttggtc
     3901 ccacgatgct aggcggctgc ctggctcgac gccgcgacgg ccgcactgac atccagcggg
     3961 ataccgggcg tcattgtact ggtcaacgta atcgaccgca aatagacacc tttctgcgcg
     4021 ctcggcttgt tgcgctggac agcgtccacg agcgcgttca gattctgcag aatgcgctgc
     4081 tcgtcgaacg acaccttgcc gatcgcgacg tgcagcaggc cggtccggtc gttgcggaac
     4141 tcgacgcggc cacccttgag ctcattcaac acgtcaggca ggtcctcagg cgtccggacg
     4201 acggtgccgg agcgcgggtt cggcatcagg ccacggcgac cgagaatacg gccgaggcca
     4261 ccaaccttgc ccatctgatc ggccatcgcg acggtcgcgt cgaactcgag ccagccgtcg
     4321 ttgatctgct tgaccagatc gtcactcccg acgtaatcgg ctccggactc ctgcgcgatc
     4381 cgggcggcat cgccgacggc g
//
LOCUS       DASUGJ010000473         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_908218, whole genome shotgun sequence.
ACCESSION   DASUGJ010000473 DASUGJ010000000
VERSION     DASUGJ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908218"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFV93_10555"
     CDS             <1..573
                     /locus_tag="VFV93_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5165825.1"
                     /translation="LNCLSGLDAVDSGTISLDGADLASMSDRQRTDYRARKMGFIFQV
                     YNLLPVLSAVENVELPLLVAGVRPKQARQKAHAALETVGLGKWANHRPARLSGGQRQR
                     VTIARALVTDPAIVWADEPTGALDSKTAIEIMSLMRELNRRDGLTFVLVTHDPGIGDR
                     CDRIVRMRDGQIAGGVSHDALAEVDAVATD"
     gene            687..>2706
                     /locus_tag="VFV93_10560"
     CDS             687..>2706
                     /locus_tag="VFV93_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5165826.1"
                     /translation="MDNILGIPVMGLMIALLILLSLCLLSVAWVAWRRPVIFKLGTRN
                     IPRRKAQTTLIVVGLMLSTLIISAALGTGDTVNYSMTSDIYDNAGQIDELVVASHERE
                     VTTVLTSDDVMAAGSLERVDGVAGNIPQIDGVMPMLDARVPVINHDAQLAEPDVVLSG
                     LDASRIGAFGGLKATDGTTIDLATLGRDTAIVSERLSGEIDAVVGDTVTVYVDDVPHN
                     LTVAAVAQDSWLSGVRRAGGGGLEFSGLAMPLESMQTLIGQPGAITSIAISNDGTVRD
                     SLDHSDTVSEALRDSLSADGYGVSALKQDRVDAGEKTATQLTTIFVILGLFSVASGIL
                     LIVLIFTMLASERRSEMGMERAVGAHRRQLVQQFVAEGSGYALLAGLVGAALGVAATF
                     GIAAGLKFFFGDYVPVEAHVEPRSMVVAYCLGVVITFATVVISSWKISRINIVAAIRD
                     MPDYVSPRRKKSTFVWATVLTLVGGFMSLVGSAPDQHMLFSTGMSILPFGIALYLRFF
                     GVPSRPVFTGVGLWILTFWLMPSNLFESIFGTYDIGIESFFTGGIFLVLGATTLIVQN
                     NDILLKGVSLAGELLRGKLPAIRTAVAYPGAAIGRTGMTIAMFSLIIFSLVMMATMNA
                     NSQASALGDSANAGWEVRADTRSSYAAAIGNIEDSLRANGVDTSGVNAV"
BASE COUNT          427 a          923 c          856 g          500 t
ORIGIN      
        1 ctcaactgcc tctccggcct cgacgctgtt gattcaggca cgatctcgct ggatggtgcg
       61 gacctggcgt ccatgtccga ccgccagcgc accgactacc gcgcccgcaa gatgggcttc
      121 atcttccagg tctacaacct gttgccggtc ctctcggccg tcgagaacgt cgagctgccg
      181 ctgctggtgg ccggtgtccg gccgaagcag gcacgccaga aggcgcacgc cgcgctcgag
      241 acggtcggcc tcggtaagtg ggccaaccac cgcccggcac gcctctccgg cggccagcgc
      301 cagcgggtga cgatcgcccg cgcgctcgtg acggacccgg ccatcgtctg ggccgacgag
      361 ccgaccggcg cgctcgactc gaagaccgcc atcgagatca tgtctctcat gcgcgaactc
      421 aaccggcgcg atggtctcac cttcgtcctc gtcacccacg atcccggcat cggcgaccgc
      481 tgtgaccgga tcgtccgtat gcgcgacggc cagatcgccg gtggtgtttc ccacgacgcc
      541 cttgcggagg tagacgccgt cgcgacggac tgatcgcgac aaaccaacct tcaacctgca
      601 aacaccaacg tcagttgtca gcggttgccg cagtgcaacc gccggcagcc gcacactgac
      661 ggctgacagc ctaaggagtt acgaacgtgg acaacatcct cggtatcccg gtcatgggat
      721 tgatgatcgc gttgctgatc ctgctctcgc tctgcctcct ctcggtggcc tgggtggcgt
      781 ggcggcgacc ggtcatcttc aagctcggca cacgtaacat cccgcgccgc aaggcgcaga
      841 caacgctgat cgtcgtcggg ctgatgctct cgacgctgat catctcggcc gcgctcggca
      901 cgggcgacac cgtcaactac tcgatgacga gcgatatcta cgacaacgcc ggccagatcg
      961 acgagctggt cgtcgccagc cacgagcgcg aggtgacgac cgtcctgacc tctgacgatg
     1021 tcatggcggc tggatcgctg gagcgcgttg acggggtcgc cgggaacatc ccgcagatcg
     1081 acggcgtcat gccgatgctg gatgcccgcg tcccggtgat caatcacgac gcgcaactgg
     1141 ccgagccaga tgttgtgctc tccggcctcg acgcgagtcg gatcggcgcg ttcggtggcc
     1201 tgaaggcgac cgacggcacg acgatcgatc tggccaccct cggcagggac acggctatcg
     1261 tctccgagcg tctctcgggc gagatcgatg ccgtcgttgg cgacaccgtc accgtctatg
     1321 tcgatgatgt gccgcacaac ttgacggtcg cggccgttgc ccaggattcc tggctctcag
     1381 gtgtgcgccg cgccggtggc ggcgggctgg agttctccgg cctggcgatg ccgctggagt
     1441 cgatgcagac cctcatcggc cagccgggcg cgatcacctc gatcgccatc tcgaacgatg
     1501 gcaccgtccg cgacagcctt gaccatagcg acacggtcag cgaggcgctg cgcgattcgc
     1561 tctcggctga tggctacggc gtcagcgcgc tcaagcagga tcgcgtcgat gccggcgaga
     1621 agacggcgac acaattgacg acgatcttcg tcatcctcgg cctcttctcg gtcgcctccg
     1681 gtatcctgct gatcgtgctg atcttcacga tgctggcgtc cgagcggcgc agcgagatgg
     1741 ggatggagcg ggccgtcggc gcgcaccgcc gccagctcgt gcagcagttc gtcgctgaag
     1801 gctccggcta cgcgctgctg gccggtctcg tcggcgcggc gctcggtgtc gcggcgacct
     1861 tcgggatcgc ggccggcctc aagttcttct tcggtgatta cgtgccggtc gaggcgcatg
     1921 tcgaaccacg cagcatggtc gttgcctact gcctcggcgt cgtcatcacc ttcgcgaccg
     1981 tcgtcatttc gagctggaag atcagccgca tcaacatcgt ggcggccatc cgcgacatgc
     2041 ccgactacgt ctcgccacgg cgcaagaagt cgacgtttgt ctgggctacc gtgctgacgc
     2101 tggtcggcgg cttcatgtcg ctggttggct ccgcgcccga ccagcacatg ctgttctcga
     2161 ccggtatgag catcctgccg ttcgggatcg cgctctacct gcgcttcttc ggcgtcccga
     2221 gccggccggt cttcaccggc gtcggcctct ggatcctgac cttctggctg atgccgtcga
     2281 atctgttcga gagcatcttc ggaacctacg acatcggcat cgaatcgttc ttcaccggcg
     2341 gcatcttcct ggtgctcggt gcgacgacgc tgatcgtcca gaacaacgac atcctgctga
     2401 agggtgtcag cctggccggc gagctgctgc gcggcaagct gccggcgatc cgcaccgccg
     2461 tcgcctaccc cggcgctgcg atcggccgga ccgggatgac gatcgcgatg ttcagcctga
     2521 tcatcttttc gctggtgatg atggcgacga tgaacgcgaa ctcccaggcg tcggccctgg
     2581 gtgactcggc caacgccggt tgggaagtcc gcgccgatac gcgcagcagc tacgccgccg
     2641 ccatcggcaa catcgaggat tccctgcgtg cgaacggtgt cgacaccagc ggtgtcaatg
     2701 ctgtcg
//
LOCUS       DASUGJ010000474         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_492536, whole genome shotgun sequence.
ACCESSION   DASUGJ010000474 DASUGJ010000000
VERSION     DASUGJ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492536"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="recF"
                     /locus_tag="VFV93_10565"
     CDS             complement(<1..890)
                     /gene="recF"
                     /locus_tag="VFV93_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX5165827.1"
                     /translation="MDGADSVRISRLELEQFRVYSDVAIDIPPRGLKIVGPNGSGKST
                     FLEAIELLSTTRPRRGSIDADVIAHGSGVDLGVQPYSRVVGTIERGDVSVRLEVFTQR
                     TGRGGSTKKLLRVADRPRRAGDVVGLVPTVTFAPDDLDLVLGSPSVRRRFLDVLLSQI
                     DRRYLRYLSRYAKILTQRNGLLRQVALEGSNGSEQFAYWDEQLIALGAYIVAARAIIV
                     QRLAAGARERFALLSERSGVLDIAYASTISANAAWWDALEASEPGTADAAQRVAVIYE
                     RQLRDGRPADLARGSTLIGP"
     gene            complement(902..2044)
                     /gene="dnaN"
                     /locus_tag="VFV93_10570"
     CDS             complement(902..2044)
                     /gene="dnaN"
                     /locus_tag="VFV93_10570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5165828.1"
                     /translation="MRVSCSQESLHRALSTAGRAVATKSTLPVLGNYLIEASGNELLI
                     SATNLEIRITVRLDAAVEKEGKATVQARVLSDFVGSLQPGEVQLDLDQGPLTVTVSQK
                     ATEAHVRGIDPDDFPPLNRTMREGTTLRLDPATLRDAIGHVVFAAASDDSRPVLAGVQ
                     LIARDETLSLAAADGFRMSLHHARLGQTVEQSLSIIVPARAMNELSRILADAGDVVEL
                     TVSPNQSQLLVSLEDIWFTTRLIDGTFPDLSQIIPKDWNTRTVISREILLDAARRAAI
                     FSRSANDVVRFDIAPASESLDMGRVTVTGNAADTGDNKDEVDAQVEGGEMQIAFNGRY
                     LTDVLSVMRDANVAFELQGANAAGVIKPADSDDFTHVIMPMVIGAN"
     gene            2243..2839
                     /locus_tag="VFV93_10575"
     CDS             2243..2839
                     /locus_tag="VFV93_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165829.1"
                     /translation="MSEQSAFDPTRHLTKVSGRDYLEVKWRLVWLRDTHPDAQIETEL
                     VQHQNQMAVFRARVTIPSGASATGWGSEGADDFGDYLEKAETKALGRALAALGFGTQF
                     CPDYDFGAERQRVVDSPVDIRSGRTSSSANGSRQEATPRQGKYLSAVAREVGLSNDEL
                     EERSQDGFGCTISELSRRDISTLIEQIQAGRPVHSIAS"
     gene            2878..3087
                     /locus_tag="VFV93_10580"
     CDS             2878..3087
                     /locus_tag="VFV93_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165830.1"
                     /translation="MVALQAQPVVALDDRESMKAEMKRLLFRLEDGYEKIAQAAVNGD
                     DVRRWEDVWLQLLCEYEELYDRMAA"
BASE COUNT          587 a          981 c          995 g          541 t
ORIGIN      
        1 tgcgggccga tcaacgtcga gccacgggcc agatcggctg ggcgcccgtc gcgaagctga
       61 cgttcgtaga tgactgcgac gcgctgggcg gcatctgccg ttcccggctc actcgcctct
      121 agcgcatccc accaggccgc attggcgctg atggttgacg cgtacgcgat gtcgaggacg
      181 cccgaacgct ccgacagcaa cgcgaaccgc tcccgtgcgc cggccgccag ccgctggacg
      241 atgatggctc gcgcagcaac gatgtaggcg ccaagtgcga tcagctgctc gtcccagtac
      301 gcgaactgct cggacccatt cgaaccttcc agggcaacct gacgcagcag cccgttgcgc
      361 tgggtgagga tcttcgcgta tcgcgacaga tagcgcaggt agcgccggtc gatctgagat
      421 aggagaacgt ccaggaaacg acgccgtacc gacggcgacc cgaggacgag gtcgaggtcg
      481 tcaggcgcga acgtgacggt cgggacgagt ccgacgacat ccccggcccg gcgcggccga
      541 tctgcgactc gcagcaactt ctttgtcgaa ccaccccgcc cggtgcgctg cgtgaagacc
      601 tccagccgaa ccgaaacgtc tccccgttcg atggtgccga cgacgcgaga gtatggctga
      661 acacccaggt ccacgccgct gccgtgtgcg atcacatcgg catcgatcga tccacgcctg
      721 ggccgtgtcg ttgataggag ctcgattgct tcgaggaagg tggacttgcc gctgccgttc
      781 ggtccaacga tcttcaaccc gcgcggcggg atgtcgatcg ccacgtcgct gtagacgcga
      841 aactgttcaa gttccagccg gctgattcga accgaatccg cgccatccac tcctgaccgg
      901 attagttcgc gccgatgacc atcggcatga tgacgtgggt gaagtcgtcg gagtctgctg
      961 gcttaatgac gccggccgcg ttggctccct ggagctcgaa ggcaacgttg gcatcacgca
     1021 tgaccgaaag cacgtccgtc aggtaacgtc cgttgaaggc gatctgcatc tcgcccccct
     1081 caacctgcgc gtcaacctcg tccttgttgt cgccggtgtc ggcggcgttg ccggtcaccg
     1141 tcacgcggcc catgtcgagc gactcggagg ccggtgcgat gtcgaagcgg acgacatcgt
     1201 tggcactgcg cgaaaagatc gcggcccggc gtgcggcgtc cagcaggatc tcgcggctga
     1261 tgaccgtccg cgtgttccag tcctttggaa tgatctggct gaggtcgggg aacgtcccat
     1321 cgatcagacg cgtcgtgaac cagatgtcct ccagcgagac cagcaactgg ctttgattcg
     1381 gcgagacggt caactcgacg acatcgccgg cgtcggcgag gatccgcgac agttcgttca
     1441 tggccctggc cgggacgatg atcgagagcg actgctcaac ggtctggccg agccgggcgt
     1501 ggtggagcga catccggaac ccgtcggcag cagccagcga gagtgtctcg tcgcgggcga
     1561 tcagctggac accggccaga accggccggc tgtcgtctga cgccgccgcg aagacgacgt
     1621 gtccgatcgc gtcgcgcaac gtcgccggat cgagccgcag cgtcgtcccc tcgcgcatcg
     1681 tgcggttcag cggcgggaaa tcatcaggat cgatcccacg gacgtgcgcc tcggtcgcct
     1741 tctggctgac ggtgaccgtc aacggcccct gatccaggtc gagctggact tcgccgggtt
     1801 gaaggctgcc gacgaaatcg ctcaggacac gggcctgaac ggtcgccttg ccctcttttt
     1861 cgacggctgc gtcaagtcga accgtgatcc tgatctccag gttcgtcgcc gagatcagga
     1921 gttcgttgcc gctggcttca atgaggtagt ttccgaggac cggcagcgtg ctcttcgttg
     1981 cgacggcccg accggcggtt gaaagcgccc gatggagact ctcctggctg caactcaccc
     2041 gcaccgctgc cactccttca gtgtccgtac acatgcccgt ggtggcgaag tatagcacag
     2101 cgaacagcga caatggaccg ttgccgaatc cgaacgcacg ttctaaaatg tgttgacaat
     2161 cggacaaggc gacagtaaaa taccttagaa cacctgttct acaggtggcg gactcaagga
     2221 tgacgtgaac ggagagatga cgatgagcga gcagtccgcg ttcgacccca cccggcacct
     2281 gacgaaggtc agcggccgcg attacctgga agtcaaatgg cgtctcgtct ggctgaggga
     2341 tacgcatccc gatgcgcaga tcgagaccga actcgtccag catcagaatc agatggcggt
     2401 cttccgcgcc agagtcacga tcccgagcgg cgccagcgca acgggctggg gcagcgaggg
     2461 cgctgacgat ttcggtgact accttgagaa ggctgagacg aaggcgctcg gccgcgctct
     2521 ggcagcgttg ggcttcggca cgcagttctg cccggattac gacttcggcg ccgagcggca
     2581 gcgcgtggtc gattcgccgg tcgatatccg tagcggccgg acttccagtt ccgccaatgg
     2641 ctcccgtcag gaagccacgc cccgccaggg gaagtacctc tccgctgtcg cgcgggaggt
     2701 tgggttgtcg aacgacgaac tcgaagagcg ttcgcaggat ggcttcggct gcacgatctc
     2761 ggagctgagc cgccgcgaca tctcgacact gatcgagcag attcaggccg gccggccggt
     2821 ccacagtatc gcgtcctgag agaagcagac gcctgattcg ggcaggagga accgtcaatg
     2881 gtcgcacttc aggcacaacc agtcgtggct ctggacgatc gcgagtcgat gaaggccgag
     2941 atgaagcgcc tgctcttccg gctcgaagat ggctacgaga agatcgccca ggcagccgtc
     3001 aacggcgacg atgtccgccg ctgggaagac gtctggctcc agcttctctg tgagtacgag
     3061 gaactctacg accgaatggc ggcgtgacaa cccgtcacaa tcta
//
LOCUS       DASUGJ010000475         9858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_74045, whole genome shotgun sequence.
ACCESSION   DASUGJ010000475 DASUGJ010000000
VERSION     DASUGJ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74045"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..2533
                     /locus_tag="VFV93_10585"
     CDS             77..2533
                     /locus_tag="VFV93_10585"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX5165831.1"
                     /translation="MSTGSLSSRAAAKRGGAGRRGTAGATQSRKANRATRTTDERDLL
                     MRSIAFLAFPALLLLITGIIAGRPPADGEIERGVSVAGIAVDGHNWDTVAASLSDRFN
                     TYLQKPIQIEVDGQIATVAPQEIGIGFDVAATHQRVVQVGRGGLLGSTVERLEAHTRG
                     IDVAPVVTIDRSKLLATLGTLGQSEITPPKDAAFSWDGAALTIEPSSEGTGIDAGAVA
                     NLLRGTVANLDHDPVGIQVVPLKPAVSTADLENVRADAAALLTEPLVVTNGEQYWQIA
                     QPALAEMLSAKDGKLALNGRALTPLIGSLETSVNREATSAAVAYNGDSTFRIEPEVAG
                     RTLDVAASVAAIQRQLLAGEHEINLVVEEAQPKIAEADLQPLLARANQIAAAGMKVSW
                     PEGEQALDSNAFAGAMLIDEASGEITFDTVAVFSMLEPIARSLNRPATGYRWIDWSIV
                     APDGALPGRLVDINATVNRVIANALGGNTTTPMVIDEQQDATAVAGEIVINDLLGTAS
                     TYYGSSGNNRRINVELAASLLDGYLVAPGAEFSFNKAIGGTATLDDGYQMGYGIIAGA
                     NGTPQTVPSVAGGICQVATTAFQAAFWSGVRIGERNWHLYWIPNYGAGPGGMTGLDAT
                     VDPDYALDNTWVNTTDSWIGINAVADGEWITVEIWGTSQGWTVHADDPVIDNVVKADP
                     TMIRQYNADVPPGQSVVVEHAQDGFTATIHRTVTNANGEQIDDLWLTSYYLPSRNVTL
                     VGDGVSLDTPAPDPTYQEPVADDPVENDPAVEEPTAEPTPEPTAEPTAEPTAEPTVES
                     TVESESTQEVTSDPTEESAP"
     gene            complement(2702..3076)
                     /locus_tag="VFV93_10590"
     CDS             complement(2702..3076)
                     /locus_tag="VFV93_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5165832.1"
                     /translation="MGQRVSFVLDTNIVIYELSDKLTQPLPVGPYAVSVISEMELLSY
                     PDLDQNEEREIRRFLTGVVVVDLTPHIQAVAIQLRRLYRLRLPDAIVAATSVSLGEEL
                     LTSDRRLARTPSLRCRLITIET"
     gene            complement(3061..3291)
                     /locus_tag="VFV93_10595"
     CDS             complement(3061..3291)
                     /locus_tag="VFV93_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165833.1"
                     /translation="MIAIRKKIVTDETNQPVAVQIDYEDWLEIEKLLSLQTDKHLLTD
                     LSEFRGVLKMTEDALEYQRRIRAEWDREWDKE"
     gene            3468..3764
                     /locus_tag="VFV93_10600"
     CDS             3468..3764
                     /locus_tag="VFV93_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5165834.1"
                     /translation="MPRYMTIGKLMMTPGHRPVAEGIADQGAPGMATMPGFQSVTFFL
                     DEERSEYGAVSVWDSREAAEQADAALTPEFEKAFGDLLQGSITTQIYEIYEHKS"
     gene            complement(3833..4264)
                     /locus_tag="VFV93_10605"
     CDS             complement(3833..4264)
                     /locus_tag="VFV93_10605"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX5165835.1"
                     /translation="MSVSLRRFVVLPALALALMIAAVGMSASRADAQEIRYPIGDWPV
                     LELTGSDLVISNVSEGYSRAGWFTSITVRNDGNVSAGEFHVGFNNSYAYVPGLNVGAS
                     STVRIYRGRSCEASGTIMADAFNEVYERNEGNNSRNWVVIC"
     gene            complement(4617..4958)
                     /locus_tag="VFV93_10610"
     CDS             complement(4617..4958)
                     /locus_tag="VFV93_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165836.1"
                     /translation="MSTEHRHFRHIEAPNGSGTLHRGEHFLATVTYGLRVLQEIIGTR
                     HWSGASAIQGTRLITGKFAITRGSLPFEGDELTLRLEDGRSLALQVVGDGPNYTIESI
                     GGFRDGSRGKE"
     gene            complement(5036..5380)
                     /locus_tag="VFV93_10615"
     CDS             complement(5036..5380)
                     /locus_tag="VFV93_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165837.1"
                     /translation="MHLETFVDAVTAVTPVPDETRPADSRMHLTGTLRQHGVDYVLEQ
                     QLDVDGGTGPRWTTPRIRRLMATDATRPALLANVDRVVTISGTPGCGDRRNRTSIVLD
                     SVQLFDAIPTPA"
     gene            5587..6243
                     /locus_tag="VFV93_10620"
     CDS             5587..6243
                     /locus_tag="VFV93_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5165838.1"
                     /translation="MSKPLRAWRAEQLLSTRRLASEAGTSNKTIVQIENGRQTPTFAT
                     IEKITRVLKVDPRDVNEFALALAERSRESIETVPASRTNAPPVVHVLCLSALASFLTV
                     ARRLLATERYSVTSMIGVPVMAEQITCLQPDLVIVDLGADPQPIIELIPQLQQTGTTA
                     PFPIVATGRDRRRLEETLSGLGAGVRQRVTLAPFDRDLRELLAMVDALAGVEVGTSAG
                     "
     gene            6288..6527
                     /locus_tag="VFV93_10625"
     CDS             6288..6527
                     /locus_tag="VFV93_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165839.1"
                     /translation="MTVTDSTSAFEAAIARYEAGGWKVRTIDRTGLRATVRAGTTAAS
                     KHAPGVMIQVAATASGCRRIWVDSKGEVQETDVPC"
     gene            6521..8458
                     /gene="treZ"
                     /locus_tag="VFV93_10630"
     CDS             6521..8458
                     /gene="treZ"
                     /locus_tag="VFV93_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX5165840.1"
                     /translation="MLEGATVLELERGTDPARVAALRRLPVGVELRAAGGVDVRVWAP
                     RCHSVAFVLESSSGHPTETPLAAEADGYFAGFVAEASPGSRYRFRLDGGSAYPDPASR
                     FQPDGPHGPSVVVDPSTFVWSDDDWAGVALRGAVIYELHIGTFTQASNWAAARAELPA
                     LRDLGVTVIEVMPIADFPGRFGWGYDGVNLFAPSRLYGTPDDARQFVDDAHRLGMGVI
                     LDLVYNHLGPDGNYLAAFSDDYRSKGEHTEWGDGFNFDRENSAPVRELFVSNARYWIE
                     EFHFDGCRLDATQQIFDDSDRHIIAEVTAAIRDAAGTRRVIVIAENERQNVQLVASPA
                     DGGCGLDGLWNDDFHHSATVALTGRNEAYYSQHHGTAQELISAAKYGFLYQGQYYAWQ
                     QQARGTPALGLPSEIAISYLQNHDQVANSARSSRIHQLTDASQLRALTALLLLGPSTP
                     MLFQGQEFAASSPFSYFADHQDELAGLVNAGRADSFRQFPSVMDFQTTSDMAVPTAMT
                     TFMASKLDHAERERNAEVYTLHRDLLRLRRDDPVFRLQAALGIDGAVIGDEALVLRFF
                     GAEGDDRLLILNLGRDVVLLPMPEPLLAPSRRGPWRLFWSSEEPRYGGSGTPPFTPNA
                     SWRLAAHSAIVLLPSRGQESA"
     gene            8825..>9858
                     /locus_tag="VFV93_10635"
     CDS             8825..>9858
                     /locus_tag="VFV93_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HEX5165841.1"
                     /translation="MSDHPHDDASADSDCVVVSVPDAGLPASGIDAQIEQDQVISVDE
                     FFLLDDWSGDAVASDAVPSDSASLGMAETRDTTRTGVVERGHDESHLDRDIAWRRHPA
                     VDVSDVNLDDPVHLYLRQIGRVPLLNADQEVELAQALERRAYLATISQQVQSDRASST
                     ADTTGDSRRLSADLCLCDTVRDHAGLIGRSVFQTFSEGWSYVAALHRVVYPDMPPQTR
                     RAMLAAVLPVTRIPEELFREVAATRGVTIDAYEEQVRRRSVEWSLLPREIQQRIEAPE
                     AMLGQEEAGQVIDAVAAHLGDVYVRMIEQADHAREHLTEANLRLVVSVAKKYAGHHLS
                     FLDLIQEGNLG"
BASE COUNT         1736 a         3004 c         3338 g         1780 t
ORIGIN      
        1 ggttgcctcc cgggctggtg gccgggcggc gggtttgtaa cgcgatctac atgaacgcaa
       61 tagggcgggc tgacgagtga gcactgggag tctttcatcg cgggcagccg cgaagcgcgg
      121 cggtgcgggg cggcggggta cggcgggcgc gacccagtcc aggaaggcga accgggcgac
      181 tcggacaacc gacgagcgtg acctgctgat gcgttcgatc gcgtttctgg cgtttccggc
      241 cctgctgctg ctgatcaccg gcatcatcgc cggccggccg ccggccgatg gtgagatcga
      301 gcgcggcgtc agcgtcgccg ggatcgccgt cgatggtcac aactgggaca ccgtcgccgc
      361 cagcctctcc gaccgcttta acacctacct gcagaagccc attcagatcg aggtcgatgg
      421 ccagatcgcc acggttgcgc cgcaggagat cggcatcggc ttcgacgtcg ccgccactca
      481 ccagcgtgtc gttcaggtcg ggcggggcgg gctgctcggc tcgaccgtcg agcggcttga
      541 ggcgcacaca cgcgggatcg acgtcgcgcc ggtcgtgacg atcgaccgta gcaagctgct
      601 ggcgaccctc gggacgctcg gccagagcga gatcacgccg ccgaaggatg ccgccttcag
      661 ctgggacggt gcggcgctga cgatcgagcc gtcgtccgag gggacaggca tcgacgccgg
      721 ggcggtcgcc aacctgctgc gtgggaccgt cgccaacctc gaccacgatc cggttggcat
      781 tcaggtcgtg ccgctcaagc cggccgtctc gaccgccgat cttgagaacg tccgcgccga
      841 tgccgccgcg ctgctgaccg agccgctggt cgtcacgaac ggcgagcagt actggcagat
      901 cgcccagccg gcgctggccg agatgctcag cgcgaaggac ggcaagctgg cgctcaatgg
      961 ccgcgcgctg acgccgctga tcggctcgct ggagacatcg gtcaaccgcg aggcgacctc
     1021 ggctgcggtt gcctacaacg gcgacagcac cttccggatc gagccggagg ttgccggccg
     1081 gacgctcgat gtcgccgcca gcgtcgccgc gatccagcgc cagctgctgg ccggtgagca
     1141 cgagatcaac ctggttgtcg aggaagcgca gccaaagatc gccgaagccg acctgcagcc
     1201 gctgctggcg cgtgccaacc agatcgcggc ggccgggatg aaggtcagct ggccggaggg
     1261 cgagcaggcg ctggacagta acgccttcgc cggggcgatg ctgatcgacg aagcgtccgg
     1321 cgagatcacc ttcgatacgg tcgccgtctt ctcgatgctg gagccgatcg cccgtagcct
     1381 gaaccggccg gcgaccggtt accgctggat cgactggtcg atcgtcgcgc cagacggggc
     1441 gctgccgggc cggctggtcg acatcaacgc gaccgtcaac cgcgtcattg ccaacgcgct
     1501 gggtggcaac acgacgacgc cgatggtcat cgacgagcag caggacgcaa ccgctgtcgc
     1561 cggtgagatc gtcatcaacg acctgctcgg gacagcctcc acctactacg gctcctccgg
     1621 caataaccgc cgcatcaacg tcgagctggc tgccagcctg ctggacggct acctggtcgc
     1681 gccgggggcc gagttctcct tcaacaaggc gatcggcggg acggcgacgc tcgacgacgg
     1741 ctaccagatg ggctacggca tcatcgccgg cgccaacggc acgccgcaaa cggtcccatc
     1801 ggtggcgggc ggcatctgcc aggttgcgac gacggcgttc caggccgcgt tctggtcggg
     1861 cgtccggatc ggcgagcgca actggcactt gtactggatc cccaactacg gcgccggccc
     1921 cggcgggatg acgggactgg acgcgaccgt tgaccccgac tacgcgctcg acaacacctg
     1981 ggtcaacacg accgacagct ggatcgggat caacgccgtc gccgatggcg agtggatcac
     2041 ggtcgagatc tggggcacgt cgcagggctg gacggtccac gccgacgacc cggtcatcga
     2101 caacgtcgtc aaggccgacc cgacgatgat ccgccagtac aacgccgacg tgccgccggg
     2161 ccagtcggtc gtcgtcgagc acgcgcagga cggcttcacg gcgacgatcc accggacggt
     2221 gacgaacgcc aacggcgagc agatcgacga cctctggctg acgagttact acctgccgtc
     2281 gcgcaacgtg acgctggtcg gtgacggcgt ctcgctggac acccccgcgc cagacccgac
     2341 ctaccaggag ccagtcgcgg acgacccggt cgagaacgac ccggccgtcg aagagccgac
     2401 ggccgagccg accccggaac caacggcaga accaactgcc gaaccaaccg ccgagccaac
     2461 ggtcgagtca acggtcgagt cagagtccac ccaagaggtc acgagcgacc cgaccgagga
     2521 gagcgcgccg tagactgatc tattgcagca tcaatgtgac tgggggcggg acgcggtctc
     2581 gccctttttc gctggctggg caggtgagca gcattgcagt tcgagtcaat cgcgcacgcc
     2641 tctccgcatg gagcaatcat gttgcgggtg acttgaagcc gtactactgg gtgtagctcg
     2701 ctcatgtctc tatcgtgatg agacgacatc gcagactcgg tgttctcgcg agtcgccgat
     2761 cgctcgtgag gagttcctcg ccgagcgaga ccgaggtcgc ggccacaatt gcgtcgggga
     2821 gccggaggcg atacaaccgg cgcagttgga ttgcgacagc ttgaatgtgc ggagtcagat
     2881 cgacaactac gacgcccgtg aggaaccttc tgatctctcg ctcttcgttt tggtccagat
     2941 ccgggtagga cagcagttcc atctccgaga tgacggatac cgcgtacgga ccgacgggta
     3001 acggctgggt gagcttgtcc gagagttcgt agatgacgat gttcgtgtcg aggacgaagc
     3061 tcactctttg tcccactcgc gatcccactc agcgcgaatt cgtcgctggt attcaagcgc
     3121 atcctcggtc atcttcagga cgccacggaa ctccgacaaa tcggtcagca ggtgcttgtc
     3181 agtttgcaag ctgaggagct tctcgatttc cagccagtct tcgtagtcga tctggacggc
     3241 gacgggttgg ttcgtctcgt cggtgacgat cttcttgcgg atggcgatca tggcgtgcac
     3301 ctctcggttt gccgcagcga ttgacggaac ggtacaccat cgcagatcgc gcttccatga
     3361 cgatcaacat caacggcggg aaggcgtagt gatccgtttg gctgggcgct gcgaaataac
     3421 ggaaacggtc gatttctgac gctggacatg attgagagga gcgcgacgtg ccacgttaca
     3481 tgacgatcgg gaagctgatg atgacgccgg gccaccggcc ggttgccgag ggaattgccg
     3541 atcagggcgc gccggggatg gcgacgatgc cgggcttcca gagcgtgacg ttcttcctcg
     3601 atgaggagcg cagcgagtat ggcgccgtca gcgtctggga ctcacgtgag gcggccgagc
     3661 aggccgacgc cgcgctgacg ccggagttcg agaaggcctt cggcgatctg ctgcagggct
     3721 cgatcacgac acagatctac gagatctacg agcacaagag ctgacatcac cccgacgcga
     3781 cgacaccccg ggcagcgtcg ctcagggtgt cgtcgtgcgt gggcgggtgt cgttagcaga
     3841 tcacgaccca gttcctggag ttgttgccct cgttgcgttc gtagacttcg ttgaaagcgt
     3901 cggccatgat cgtgccgctg gcctcgcagg agcggccgcg atagatgcgg acggtgctgc
     3961 ttgcgccgac attgaggccg gggacgtagg cgtacgagtt gttgaacccg acgtggaatt
     4021 cgcccgcgga gacgttgccg tcattgcgga cggtgatcga ggtgaaccag cctgcacggc
     4081 tgtagccttc ggagacgttc gaaatgacca ggtcggagcc ggtcagttcg aggaccggcc
     4141 agtcaccgat cgggtagcgg atttcctggg cgtcggcgcg cgaggccgac atgccaacgg
     4201 cagcgatcat cagggcgagg gcgagcgcgg ggagaaccac gaagcgacgg agtgagacgg
     4261 acatgggtgg ctccttcctt ggaagctgca cggtccacat cgaccgtggc ggaagtacac
     4321 ccgattccgt ccatccggac agcgtcgatc ctgacagacc ggctgttaac tcggacacag
     4381 ccggcctgct ggcaggctac ggttgtggtc tgcctggcgg gttctcgccg tggttggatg
     4441 gattcgttcc gaggtgggac tggttggttc cctgggttgg ccgaggtccg gcaatcatgc
     4501 gacggatcgt gtcgagcagt ttgcgtagca tggctggtct ccgtccttgt gtccgcacag
     4561 gtgctgctgt gcgggtcgtc gctccagcgc gtgctgaagc gtcctgtggc tggtcgttac
     4621 tccttgccgc gcgatccatc gcggaatcca ccgatcgact cgatggtgta gttggggccg
     4681 tcaccgacga cctggagcgc caggctgcgg ccgtcttcga ggcgcaaggt caattcatct
     4741 ccctcgaacg ggaggctgcc gcgtgtgatc gcgaatttac cggtgatcag gcgcgttccc
     4801 tggatcgcgc tcgcgccgga ccagtgtcgt gtgccaatga tctcctggag gacccgcaac
     4861 ccgtaggtga cggtcgccag aaagtgttcg ccacggtgga gtgtgccgga gccgttcggc
     4921 gcctcaatgt ggcgaaagtg tctgtgttct gtcgacatcg tgcccccttc tcttgctgtg
     4981 gatcgttccg tcgccgcgat gagcgatgtt tcaggcgtca tctcacgcgt gccgatcagg
     5041 cgggggtagg gatcgcatcg aatagctgga cgctgtcgag gacgatcgag gttcgattcc
     5101 gccggtctcc gcaaccgggt gtccccgaga tggtgacgac gcgatcgacg tttgcgagga
     5161 gcgctgggcg ggtcgcgtcg gttgccatca gccggcgaat cctgggcgtc gtccagcgtg
     5221 ggccggtgcc cccgtcgacg tccagctgct gttcaagcac atagtccacg ccgtgttggc
     5281 gcagggtgcc ggttagatgc atccgtgaat cagcaggtcg tgtctcgtct gggactggtg
     5341 tgacggctgt gacggcgtcg acgaatgtct caaggtgcat ctgggtatgc cgatctgcgc
     5401 tcgcgcgcga taggggaacg tgctgtgccg gtagtcggcg caacgggtcg accgcaaaat
     5461 gtgttgtgtc gcaggccagc tcatgacgat cttcgagata acaccattat gataccatac
     5521 taacagcgat gcaaatatga tgtagatatg gtgtagtact tcgtctacta gcaggagagc
     5581 gatgcgatgt cgaagccatt gcgggcctgg cgcgccgagc agttgctgag tacccgacgg
     5641 ctggcgagtg aggccggcac ctcgaacaag acgatcgtcc agattgagaa cgggcggcag
     5701 acgccgacgt tcgcgacgat cgagaagatc acgcgggtgc tcaaggttga tccgcgcgat
     5761 gtgaacgagt tcgcgctggc acttgccgaa cgatcgcgcg agtcgatcga gaccgtgcct
     5821 gcctcgcgaa cgaatgcgcc gccggtggtc catgtcctgt gcctcagcgc actggcgtcg
     5881 tttctcactg tcgcgcgccg gttgcttgcg acggagcgct acagcgtcac aagcatgatc
     5941 ggtgtgccgg tgatggcgga acagatcacc tgcctgcagc ccgaccttgt gatcgtcgac
     6001 ctgggcgccg acccgcaacc gatcatcgag ctgatcccgc agctgcagca gaccgggacg
     6061 acggccccgt tcccgatcgt cgcgacaggt cgagatcggc ggcggctgga ggagacgctt
     6121 tccgggctgg gtgccggcgt gaggcaacgg gtgacacttg cgccgttcga ccgtgatctg
     6181 cgtgaactgc ttgcaatggt tgacgcgttg gcaggcgttg aagttggcac gtcggccgga
     6241 tgacaccggc gccagactgc acagcgagta tgaaggatga ggacatcatg acggtgacag
     6301 attcaacgag cgcgttcgag gcggcgatcg cgcgctacga agccggtgga tggaaagtgc
     6361 gtacgatcga ccgcacggga cttcgggcaa cggtccgggc cggcacgacg gcggccagca
     6421 agcacgcgcc tggagtcatg attcaggttg ccgcaacggc gtccggatgc cggaggatct
     6481 gggtcgacag caagggcgag gtgcaggaga cggatgtccc atgttagaag gggccacggt
     6541 gctggaactg gagcgcggga ccgatccggc gcgtgtagca gcactgcgtc ggctgccggt
     6601 cggcgttgag cttcgcgcgg cgggcggcgt cgatgtgcgc gtctgggcgc cacgctgcca
     6661 ttcggtcgcg tttgtcctcg aatcgtcgtc tggtcatccg acggagacgc cactcgccgc
     6721 cgaggcggat ggctacttcg ctggattcgt ggctgaggcg agccctggca gccgctatcg
     6781 gtttcggctc gatggcggct cggcgtaccc cgatcctgcc tcccgtttcc agccggatgg
     6841 gccacacggc ccgtcggtgg tcgtcgatcc gtctacattc gtctggagcg atgacgactg
     6901 ggccggggtc gcactacgcg gcgcggtgat ctacgaactg cacatcggaa cgttcaccca
     6961 ggcgagcaac tgggcggcgg cccgggccga gttgccggcc ctgcgcgacc tcggtgtgac
     7021 ggtgatcgag gtgatgccga ttgccgattt ccccggccgg ttcggttggg gctatgacgg
     7081 tgtcaacctg tttgcgccgt cgcggctgta tggcacaccc gacgatgcgc ggcagtttgt
     7141 ggatgacgcc caccggctcg gcatgggcgt gatcctggat ctggtttaca accatctcgg
     7201 cccggacggt aactacctgg cggccttcag cgacgactac cgttcgaagg gcgagcacac
     7261 tgaatggggc gacgggttca acttcgatcg cgagaacagc gctccggttc gtgagctgtt
     7321 cgtcagtaac gcacgctact ggatcgagga gttccacttc gacggttgcc ggctggatgc
     7381 aacacagcag atcttcgatg actcggaccg gcacatcatc gccgaggtga cggcagcgat
     7441 ccgtgacgca gccgggacgc ggcgggtgat cgtcattgcc gagaatgagc ggcagaacgt
     7501 gcagctcgtc gcgtcgcctg ccgacggtgg ctgcggcctg gacggcctct ggaacgatga
     7561 tttccatcac agcgccaccg tcgcgttgac cggccgcaat gaggcctact acagtcagca
     7621 tcatggcaca gcgcaggaac tcatctcagc ggcaaagtat ggattcctgt atcagggaca
     7681 gtactacgcc tggcagcagc aggcgcgcgg cacaccggcg cttggcttgc cgtccgagat
     7741 tgcgatttct tatcttcaga accacgatca ggttgctaac tctgcccgta gctcgcgcat
     7801 tcaccaacta accgacgcga gccaactgcg ggcgctgacg gcgctgctgc tgctgggtcc
     7861 aagcacgccg atgctgtttc aggggcagga gttcgcggcg tcgagcccct tcagctactt
     7921 cgccgaccac caggacgaac tggccgggct ggtcaacgcc gggcgcgctg actccttccg
     7981 gcagttcccg agcgtgatgg actttcagac aacgtcggac atggccgtgc cgaccgcgat
     8041 gacgacgttc atggcttcca aactcgatca cgcggaacgg gagcgcaatg ccgaggtata
     8101 tacgctccat cgcgacctcc tgcggctgcg gcgggatgac ccggtcttcc ggctccaggc
     8161 ggccctgggg atcgacggcg ccgtcattgg cgacgaggcg ctcgtgctgc gtttcttcgg
     8221 cgcggaaggc gacgaccggc tgttgattct caatctgggc cgggacgtcg tcctgctacc
     8281 gatgccggaa ccgctgctgg cgccgtctcg tcgcgggccg tggcggttgt tctggtcgag
     8341 tgaggagccg cgatacggcg gcagcggcac gccgccgttt acgccgaacg cgtcctggcg
     8401 gctggccgcc cactccgcga tcgtgttgct gccatcgcgc ggccaggaat cggcgtagca
     8461 tcccggacat gatcggcggc gcgacgatgg tgtcctcgtc caactgagcg atgcgtctcg
     8521 gcttcgatcg acgctgatgc tggtctggct ggcgtgaccc gctgtcgcgt gaacgcgtgt
     8581 cagatcgtcg agagctggac cgactgggat cgcgatgcgg ccctgcgtca cctggggtgg
     8641 accggtggtc gggatgcgaa agcgaaccca ctcatgtaac gcgcactggt gtcgcatcgg
     8701 tatgtgcgcc aaatgggcat caattcacct gatgttacac agttcatgtg acgaggttgt
     8761 gtataatttc cctacgcaga tttcattgtc atttgcgaga cgcagaccga aacggagcta
     8821 ctccgtgtcc gatcatcccc acgacgacgc cagcgcggac agcgattgcg tggttgtttc
     8881 cgttcctgac gccggtctac ctgcatcggg tatcgacgca cagattgagc aggaccaggt
     8941 gatctcagtc gacgaattct tcctgctgga tgattggtcg ggggacgcgg tggccagcga
     9001 cgcagtcccg tccgattcag catcgctcgg aatggctgag acccgcgata ccacacgaac
     9061 gggtgtggtg gagcgcggcc atgatgagtc ccatctggat cgagatatcg cttggaggcg
     9121 tcacccggca gtggacgtct ccgacgttaa cctcgatgat ccagtgcacc tctatttgcg
     9181 tcagattggt cgcgtgccgc tgctgaatgc cgatcaggaa gtcgagttgg cacaggcact
     9241 ggagcgccga gcctacctcg cgaccattag ccagcaggtg caatccgacc gagcgagcag
     9301 cacggcggac acgaccggcg attcgcggcg attgtccgct gacctgtgct tgtgcgacac
     9361 cgtccgggat cacgcaggcc tgatcggtcg tagcgtcttc cagaccttca gcgaaggctg
     9421 gtcatacgtt gctgcgctgc atcgagtggt gtatcccgac atgccaccgc agaccaggcg
     9481 tgcgatgctg gcggctgtcc tgccggtcac tcggatcccc gaggaattgt tcagggaggt
     9541 ggccgccacc cgaggggtca cgatagacgc atacgaggag caagtgcgac gacgctctgt
     9601 cgagtggtcg ctgctgccac gcgagatcca acagcggatc gaggcgccgg aagcgatgct
     9661 cggccaggag gaggcaggtc aggtcattga cgctgtcgcg gcgcatctcg gcgatgtcta
     9721 tgtgcggatg atcgagcagg ctgatcacgc gcgtgagcac ctgaccgagg caaacctgcg
     9781 gctggtcgtc agtgtggcca agaaatacgc cggacaccac ctgtcattcc ttgacctgat
     9841 ccaggaaggc aatctcgg
//
LOCUS       DASUGJ010000476         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1463372, whole genome shotgun sequence.
ACCESSION   DASUGJ010000476 DASUGJ010000000
VERSION     DASUGJ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463372"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..272)
                     /locus_tag="VFV93_10640"
     tRNA            complement(199..272)
                     /locus_tag="VFV93_10640"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(236..238),aa:Asp,seq:gtc)
     gene            complement(774..1454)
                     /locus_tag="VFV93_10645"
     CDS             complement(774..1454)
                     /locus_tag="VFV93_10645"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEX5165842.1"
                     /translation="MGLAVVGVVPAAIGLLLVNPVPVVAVILLLFSPRARTTAPAFVA
                     GWIVGLLVVFGFVLVVASGDDLGNESEPTTLASIVRLLLGMALLYLAYRQWLGRPAPD
                     DEPTVPVWMLKLEQANPLAAFGTGALLSSLSPKNLPFTISAAVSVAQAELVGGEQLIP
                     IVVYVLLASAGIAAPMTWYFATQDHATRTLAGWRVWLAANYTTMMAIVLLMFGVILSM
                     QGLGELVG"
     gene            complement(1500..2183)
                     /locus_tag="VFV93_10650"
     CDS             complement(1500..2183)
                     /locus_tag="VFV93_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165843.1"
                     /translation="MTDDPEEMAPIGTDLSRMQTLEHDLMRYLDITSAILLSIATVAT
                     AWCVYQATGWGGEQSRAYAEASSSRIESTRSYNRAMQLLGIDADLFMGWVDAYNDGDQ
                     QLLTFYESNLMRDEFLPFLNDWKASRPLTNPDAHPNPLVSEEYQRQLFADSIQLRGEA
                     EAQFEEATEANRTADEYVLSTVIFASVLFFAGIAPKFASSRIQMALIGMAILAFAAGF
                     IQIGGLPTQ"
     gene            complement(2176..>2703)
                     /locus_tag="VFV93_10655"
     CDS             complement(2176..>2703)
                     /locus_tag="VFV93_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165844.1"
                     /translation="VAPTVIWTMSQIREGRVPGVIESYRHGVREIVPIFDTRVRVTIQ
                     IVLRALTIIGLPRAIRQIIQATFIAQAIVHEKDDPDKAVIDSARAADVNLSRTVLTQI
                     VLNVITLLTGPLVTIFLLLAIPSRPLELINFVSSFIFAFIYPFGVIGMTLLYFELRPD
                     ESRYPVGSMRGAGDD"
BASE COUNT          551 a          823 c          836 g          493 t
ORIGIN      
        1 cgcgcatagc cgagttcctg tccgacctgc cgcaagcatg gcgagcagcg aatcaggagc
       61 agcgcaacgc gctcggtcgc ctgctgtttg agtcggtgga gattcaaaag caaacggtcg
      121 tggctgtgac accacgaccg gagtttgccc cgtttttcgt cctcgactgt caacatcgag
      181 gccgtaagtt ggaacctgcg gaagtgacgg gattcgaacc cgcgatctca gccttgacag
      241 ggctgcatgt taggccgcta caccacacct ccatcgcgac ctaaaccata ccatcggggt
      301 tggctgggtg tcaaggtttt tcgccgcctg gacgtgcgaa cgaatgccga aaatagggta
      361 ttgacagcct gtcaatccca tgctattgta cgtacacgat cccgatgagt tatgacggat
      421 cggcgcaccg aggccagacg agcaatcgac tggcggaggg acttccggag acggaatggc
      481 gcggctcagc aagtcggttg ggtgcaagcc tgctgcttga agagttcgcc agacgcagca
      541 ccggagggcg agtacgttgg cttcggccag actgctcgac gcgtggcagt gagttctgag
      601 atcaccggct tgccggcgtt cccagcacga accagccgat gcggaagcgc cggatccaga
      661 gcggctcgtc gggatcgtgc ttttgtctcc agacagcagc gaaaacgttt cgatggagct
      721 cccgttcgtg ggatttcttg agcgagacgc ttccattgac gggccctcaa gctctacccg
      781 acaagttcac cgagcccctg catcgacaag ataaccccga acatgagtaa cacaatcgcc
      841 atcatcgtcg tgtagttggc cgccagccag acgcgccagc ctgccagtgt ccgcgtcgcg
      901 tggtcctgag tggcgaaata ccaggtcatg ggtgcggcga tgccggccga cgccaacagc
      961 acatagacga cgatcggaat cagttgttcg cctccaacga gctctgcctg agctacggag
     1021 acggcagccg agatcgtgaa gggcaggttc ttcgggctga ggctcgacag gagcgcaccg
     1081 gtcccgaatg ccgccagcgg gttcgcttgc tccagcttca gcatccagac cggaactgtc
     1141 ggttcgtcat ccggcgcagg ccggcccagc cactgtcggt aggcgagata cagcagcgcc
     1201 atcccgagca ggagccgcac gatggacgcg agcgtagttg gctcgctctc gttccccagg
     1261 tcgtcgcccg acgcgaccac gaggacgaac ccgaagacga cgagcagccc gacgatccag
     1321 ccggcgacga acgcgggcgc cgtcgttcgt gccctcggcg agaacagcag caggatgacc
     1381 gcgacaaccg gcaccgggtt caccagcaac agaccaatcg cggctggcac gactcctacg
     1441 actgccagtc ccaaagtacc tcctcagggg tccagttcgg tatacacgac gttggtgtct
     1501 cactgggtcg gtagcccgcc gatctggatg aagccggctg cgaatgcgag gatcgccatt
     1561 ccaatcagcg ccatctggat gcgcgaggag gcgaactttg gagcgatgcc tgcgaagaag
     1621 aggaccgatg cgaagatgac ggtcgagagg acgtactcgt cggccgtccg gtttgcctca
     1681 gtcgcttctt cgaactgcgc ttcggcctcg ccacgcaact ggatactgtc ggcaaacagc
     1741 tgccgctgat attcctcgct gacaagcggg ttgggatgtg catcggggtt cgtgagtggc
     1801 cgggacgcct tccagtcgtt cagaaacggc aggaactcgt cgcgcatcag attcgattcg
     1861 tagaacgtga gtagctgctg atcgccgtcg ttgtacgcgt cgacccagcc catgaagagg
     1921 tcggcgtcga tcccgagcag ttgcatggcg cggttgtacg atcgcgtcga ttcgatgcga
     1981 ctgctcgacg cctccgcata cgcccgcgac tgttcaccgc cccagcccgt cgcctgatag
     2041 acacaccagg ccgttgcgac ggttgcgatc gagagcagga tcgcggaagt gatatcgaga
     2101 tagcgcatca gatcatgttc gagggtctgc atgcgggaca gatccgtccc gattggcgcc
     2161 atctcctcag ggtcgtcagt catcgcccgc acctcgcatc gaaccaaccg gatatcggct
     2221 ctcgtctggc cgcaactcga agtacagcag cgtcatcccg atcaccccga acgggtagat
     2281 gaacgcgaag atgaagctgc tgacgaagtt gatcagctcc aatggacgcg atggaatcgc
     2341 gagcagcagg aagatcgtca cgagcggtcc ggtcaggagc gtgatgacgt tcaggacgat
     2401 ctgcgtcagt accgtccgcg agagattcac gtcagcggct cgcgcgctgt cgatgacggc
     2461 cttgtcgggg tcatccttct cgtgcacgat cgcctgggcg atgaacgttg cctgaatgat
     2521 ctgccggatg gcgcgcggca agccgatgat cgtcagcgct cgcagcacga tctggatggt
     2581 gacgcgcacc cgtgtgtcga agatcggcac gatctcacgc actccatgcc ggtatgactc
     2641 aatcacaccc gggacacgtc cttcccggat ctgcgacatc gtccagatca cggttggggc
     2701 gac
//
LOCUS       DASUGJ010000477         4667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_353228, whole genome shotgun sequence.
ACCESSION   DASUGJ010000477 DASUGJ010000000
VERSION     DASUGJ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353228"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /locus_tag="VFV93_10660"
     CDS             <1..1689
                     /locus_tag="VFV93_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX5165845.1"
                     /translation="RQGPFSVDGAIFIIGQVTSALDYAHHRGLIHRDIKPQNILVDRD
                     GNAKVVDFGIAKGMRDVNLTEAGTGMGTVHYVSPEQAQGEPAGPASDLYSTGVVLFEM
                     LTRRLPFEADTPVGVAMQHVNTPPPPPSTFNPRIPPEVDAIVLRSLAKEPSERYPSGA
                     ALSTALRHWDMLPPAHMPYDEPARPPAHSGTTALPPRVRTSSPPQPRNQPRPDRRTAS
                     GPPRQRTGRMPTVPPAVPPVRGAGGARATYPPYRTTARSNRDDLGCVTWLIGSAILLG
                     IVGLVVLAFKLGPGAFADDPQPTATVAVGVPTATRAPEEPTATIPPAAPTATTAPEAP
                     TATATVPAPTATATVPTGSVPRLTNLTREQAENTIGDQWVLNVVEEYSSSVSAGVVIS
                     QDPAPGASLPLGETVTIVVSRGAQVVSIPDVRGATVDDATAVLEDLGFVVAVAEEASG
                     SVPEGQVIRTDPVTEAESGSTVTLVVSSGQSDTVVVPYVYGQDVEDAVAALEAAGLNV
                     TKVTPLSCNRILQFSPDFDCDEFPDGGVVTSTLAWGSEVPRGSPIDITWFDDDL"
     gene            1686..2576
                     /locus_tag="VFV93_10665"
     CDS             1686..2576
                     /locus_tag="VFV93_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165846.1"
                     /translation="MSAPAADPAIRRRELVLAGIAALIAFVVPLSVVSWLDDPEPEIR
                     VLASSERLSALVIDGDARVLIANSDDREEAGAMLGRIAQPWEPEPRTIVAPPDEGGSL
                     SLWEALQRLEPATVVVAGIPGADPLWAAIESECNRRNIDLRYVSDRATLSTEHLDLTV
                     FGTPPEAEGATGVVIRRGEISVVAAFGSIPPPVPGQVLIFNGDPSSTTPDLLVSSDDA
                     PHSVQHNELLVTGRRYTRLVLTTDDVHVFGGTLRLPQSPNQSVAPTDCGNCPDQRTVP
                     QFGQIVTPGARTAWQVRQRS"
     gene            complement(2498..3577)
                     /locus_tag="VFV93_10670"
     CDS             complement(2498..3577)
                     /locus_tag="VFV93_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX5165847.1"
                     /translation="MSIFLVLDVLLVLLIALFTPIGFWRGPVKELFVTLGIIFGILLA
                     DFWARPWGRDLSDLSDLTAGSGAFIVAMCFLVASTFILGYGIGAALGPAYHSTEARLL
                     GAVIAALNGTLLLSFALQYVRLYLLSDANEESLNDSYVSQFLLDQIGWVLMVAAIVAV
                     PLLLYILISGRRAYELIYDDGYDDYVPVPAPPRQSVNYERARAANAATSTLPPRVPGP
                     PREADQRGYKAEPEPRPQRATAETRPLVVSEPDETAEPAPMLLDTGSDQPRPAFSDTD
                     PHIVIPAEARRPAQPETPSEPDQPEATAQPASVAASPEPQPTPEPPAASERTSDLAPG
                     YERCRTCHAVLAPGVTICPNCGTVR"
     gene            3862..>4667
                     /locus_tag="VFV93_10675"
     CDS             3862..>4667
                     /locus_tag="VFV93_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165848.1"
                     /translation="MGRSRRSRASDRGQRDHHELVWRRIDLHIHTPASADYQEPDCSF
                     LDILRKAEARGADLIAFTDHNSVRGYANLWREIEDLELLEALERLTPGEQRRIDEYRR
                     LLRSIRVLPGFELTATFGFHILAIFPEGTSIRQLEHMLLELNVPEDKIDYGSGEVGAT
                     SDVLRAYELLHDAGALVIPAHVNSTHGVAMTNLPFGGQTKIAFTQSEYIHALEATDME
                     STSRRSTARFFNGSKPEYPRRMHIIQGSDAHRLNRDPNRPSNLGACDRMT"
BASE COUNT          786 a         1593 c         1542 g          746 t
ORIGIN      
        1 cgccaggggc cgttctcggt cgatggtgcg atcttcatca ttggccaggt cacctcggcg
       61 ctcgactacg cccaccatcg tgggctgatc caccgcgaca tcaagccgca gaacatcctc
      121 gtcgatcgcg acggcaacgc caaggtcgtc gacttcggca tcgccaaggg catgcgcgac
      181 gtcaacctga ccgaggccgg gaccgggatg ggcacagtcc actacgtctc gccggagcag
      241 gcccagggcg agccggccgg ccccgcttcc gatctctatt cgaccggtgt cgtcctgttc
      301 gagatgctaa caagacggct gccgttcgag gctgatacgc cggtcggcgt cgccatgcag
      361 cacgtcaaca cgccgccacc gccgccgtcg accttcaacc cgcggattcc gcccgaggtg
      421 gacgccatcg tcctgcgctc gctggccaag gaaccctcgg aacgctaccc gagcggggcg
      481 gcgctgtcga ccgcgttacg acactgggac atgttgccgc cagcgcacat gccctacgac
      541 gaaccggccc gaccgccagc gcacagcggt acgaccgcgc tgccgccgcg agtccgaacg
      601 tcgtccccgc cccagccgcg caaccagccg cgtccggatc gccgaaccgc gtccggcccg
      661 ccacgacagc gaaccggcag aatgccgacc gtgccgccgg ccgtcccgcc tgtccgtggc
      721 gctggcgggg cgagagcgac ctacccgccc taccggacca ccgcgcgctc gaaccgcgac
      781 gacctcggct gcgtgacctg gctgattggc tcggcgatcc tgctcgggat cgtcggactg
      841 gtcgtcctcg ccttcaagct cggccctggc gcgtttgccg atgacccgca gccgacggcg
      901 acggtcgccg tcggcgtccc aaccgcgacg cgggcacccg aggaaccgac cgccacgatc
      961 ccgccggcgg ctccaaccgc cacgacggcg cccgaagcac cgacagcaac cgcgacggtt
     1021 cccgcgccga ccgcgacggc caccgtgcca accggctctg ttccacggct gacgaatctc
     1081 acccgcgagc aggccgagaa cacgatcggc gaccagtggg tgctgaacgt cgtcgaggag
     1141 tactcgtcgt cagtctcggc cggcgtcgtc atcagccagg atccggcgcc gggcgcgtcg
     1201 ttgccactgg gcgagacggt cacgatcgtc gtcagccgtg gcgcgcaggt cgtctcgatc
     1261 cccgacgtgc gcggcgcgac ggttgacgac gcgacggccg tcctggagga cctggggttt
     1321 gtcgttgccg tcgctgagga agccagcggc tccgttcccg aggggcaggt catccggacc
     1381 gacccggtca ccgaagctga gtccggctcg acagtgacgc tggtcgtcag tagcggccag
     1441 tcagacacag tcgtcgtccc gtacgtctac ggtcaggacg tcgaggacgc ggttgccgcg
     1501 ctggaggcag ccggactgaa cgtgacgaag gtgacgccgc tgtcgtgcaa ccgcatcctg
     1561 cagttcagcc ccgatttcga ctgcgacgag ttccccgatg gcggcgtcgt caccagcacg
     1621 ctggcctggg ggtcggaagt cccacgtggc tccccgatcg acatcacctg gtttgacgac
     1681 gacctgtgag cgcgccggcg gcagatcccg cgatccgtcg tcgtgagttg gtcctggccg
     1741 gtatcgcggc cctgatcgcg ttcgttgtcc cgctgtcggt cgtcagctgg ctggatgatc
     1801 ccgaacctga gatccgggtg ctggcgtcgt ccgagcgcct ctccgcgctc gttatcgacg
     1861 gcgacgcccg cgtcctgatc gcgaacagcg acgaccgcga ggaggccggc gcaatgctgg
     1921 gccgcatcgc ccagccctgg gagccggagc cgcgcacgat tgtcgccccg cccgatgagg
     1981 gcggctcgct gagtctctgg gaggcgttgc agcgcctcga acctgccacg gttgtcgtgg
     2041 ccggcatccc cggcgccgac ccactctggg ccgcgatcga atccgaatgc aaccgccgca
     2101 acatcgacct gcgctacgtc agcgaccgcg ccacgctctc aaccgagcac ctcgacctga
     2161 cggtcttcgg aacgcctccg gaggccgaag gcgctaccgg cgtcgtcatc cggcgtggcg
     2221 agatcagtgt cgtcgccgcg ttcggcagca tcccgccgcc ggtgcccggc caggtgctga
     2281 tcttcaacgg cgatccgtcc tccaccacgc ccgatcttct cgtcagcagc gacgatgctc
     2341 ctcactctgt ccagcacaac gagctgctgg tgacgggccg tcggtacacg cggctggtcc
     2401 tcacgacgga cgacgtgcac gtcttcggcg gcacgctgcg attgccacaa tcgcccaatc
     2461 aatccgtcgc ccctacggat tgtgggaatt gtcccgatca gcgcactgtg ccgcagttcg
     2521 ggcagatcgt gacgccgggg gcgaggaccg catggcaggt gcggcagcgt tcgtagccag
     2581 gggcgaggtc ggaggtccgt tccgatgctg cgggtggctc cggcgtgggt tgcggctcgg
     2641 gcgacgccgc gacggaggcc ggctgggctg ttgcttcggg ctggtcgggt tcggacggcg
     2701 tttccggctg ggccgggcgg cgcgcctcgg cggggatgac gatgtgcggg tcagtgtcgc
     2761 tgaaggcggg tcgcggctgg tcgctgccgg tgtcgagcag catcggggca ggttcggccg
     2821 tctcgtccgg ctcactgacc accagcgggc gcgtctcggc cgtggcccgt tgcggtcgcg
     2881 gctccggctc ggctttgtag ccgcgctggt ccgcttcgcg gggcgggccc ggtacgcgcg
     2941 gcggcagggt tgacgtggcc gcgttggcgg cgcgcgcgcg ctcgtagttg acggactggc
     3001 ggggcggggc cgggacaggg acgtagtcgt cgtagccgtc atcgtagatg agctcgtagg
     3061 cgcgtctgcc ggagatcagg atgtagagca gcagcggcac ggcgacgata gccgccacca
     3121 tcagcaccca gccgatctga tcgagcagaa actgggagac gtacgagtcg ttcagtgatt
     3181 cctcgttggc gtcggacagg agatagagac ggacgtactg gagcgcgaat gagaggagca
     3241 gcgtcccgtt cagggcggcg atcacggcgc cgagcagacg tgcctcggtc gagtggtagg
     3301 ccggccccag cgccgcgccg atcccgtagc cgaggatgaa ggtcgaggcg accaggaagc
     3361 acatcgcgac gatgaatgcg ccgctgccgg ccgtcaggtc gctgaggtcg ctcaggtcgc
     3421 gcccccaggg ccgtgcccag aagtcggcca gcagaatgcc aaagatgatg ccgagtgtaa
     3481 cgaacagttc cttgaccggt ccgcgccaga agccaatcgg ggtgaagagt gcgatgagta
     3541 ggaccaacag gacatcgagt acgaggaaga tagacatgtg gctcccgcca tattgctgct
     3601 gcagaagtgc tgccgcgccg gattctcaga cggcgctacc gcaaggtggc attcgcacag
     3661 acgtcgcaac gttcgcgcgt tcacgaacgg catcgagtgt tcgcagcgag tctaacacgc
     3721 gctcgttagt gaacgtacac agtgttacgt gatgtttccg gcagattgat acaatttcca
     3781 caggtatggc cgttcgatgg ccagccggcc ggcctgacat tttgccggtc catcgcactc
     3841 aacgaagggt attgacactc attgggcagg agcagacgct cccgcgccag tgatcgcggc
     3901 cagcgggacc atcatgaact ggtctggcga cggattgatc tgcatattca tacccccgca
     3961 tcagccgatt accaggaacc cgattgttcg tttctggaca ttcttcgcaa ggctgaagcg
     4021 cgtggagcag atctgatcgc cttcaccgac cacaattcgg tccgtggcta cgcgaacctc
     4081 tggcgggaga ttgaagacct cgaactgctc gaagcactcg aacgattgac acccggcgaa
     4141 cagcggcgga ttgacgaata tcgtcgcctg ctgcgctcca tcagagtcct gccgggcttc
     4201 gagttgacgg caacgttcgg tttccacatt ctggcgatct tcccggaagg aacctccatc
     4261 cggcagctcg agcatatgct gctggaactc aacgtccccg aagacaagat cgactacggc
     4321 agcggcgagg tcggcgctac cagcgacgtc ctgcgcgcct acgaactgct gcacgacgcc
     4381 ggcgcgctcg tcatccccgc ccacgtgaac tcgacccatg gcgtcgcgat gacgaacctg
     4441 ccgttcggcg gccagaccaa gatcgccttt acccagagcg agtacatcca cgcgctcgaa
     4501 gcgaccgaca tggagagcac cagccggcgg agcacggccc gcttcttcaa cggctcgaag
     4561 ccggagtatc cccggcggat gcacatcatc cagggatcgg acgcccaccg gctcaaccgc
     4621 gacccgaacc gccccagcaa cctcggcgcc tgcgaccgga tgaccga
//
LOCUS       DASUGJ010000478         6561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_630256, whole genome shotgun sequence.
ACCESSION   DASUGJ010000478 DASUGJ010000000
VERSION     DASUGJ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630256"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..676
                     /locus_tag="VFV93_10680"
     CDS             260..676
                     /locus_tag="VFV93_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX5165849.1"
                     /translation="MADYKKPLPMPDPVTQPFWDSTKAHAMKIQRCNDTGKFFFYPRG
                     LSPFTLSDNISWEPVSGKGTLHAFTIVQNQRQPGFAEEVPYVVAMVELDEGVRMMTNL
                     IGVEPDPAHVKIGMPVEVVYEDVSEEITLPKFKPAG"
     gene            764..1015
                     /locus_tag="VFV93_10685"
     CDS             764..1015
                     /locus_tag="VFV93_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165850.1"
                     /translation="MDQVIQVVGAMMILAGFVLAQFKVLRVESMTYLVLNVIGSAILA
                     WLAWDDRQWGFLLLEGVWAIVSAWSIIQLLRGGDVPSAH"
     gene            complement(1049..1765)
                     /locus_tag="VFV93_10690"
     CDS             complement(1049..1765)
                     /locus_tag="VFV93_10690"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5165851.1"
                     /translation="MKVGELARRTGLTVRTLHHYDEIGLLSPSTRTEAGHRLYSDADV
                     LRLQQIASLRQFGLPLDEIRELLAGRDRSALPVIEMHIARVRDQVRLQQRLLGQLDQI
                     AGHLRRNGTAPVDELLRAMEMMTMVNTYYSEEQLEWLRKRREEVGEDRIREVEAEWPR
                     LMAEVRAEMVAGTDPADPKLQPLLDHWGGLVAEFTGGNDGIAQSLTNLFENEDSLPDG
                     QQIDRELFAYVGKAMAARNP"
     gene            complement(1944..2759)
                     /locus_tag="VFV93_10695"
     CDS             complement(1944..2759)
                     /locus_tag="VFV93_10695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5165852.1"
                     /translation="MSSGYTDSFKGTAAWYARYRPGYPDAMIAHVIDRFGLDGSGRLL
                     DLGCGTGQLLIPLAPHVETAIGMDPEPEMLNEAQRVAEAAGITNAVWIEGGSLDLERL
                     HDQLAPLRLTTIGRAFHWMDGDATLSALGALTVPGGGVVQCGEPCEIWSGQRDWQVAV
                     REVIQRWLGEERRAGSGTYAARSDRIPWSEGDLLARSPFSQVEFQEFAYTRRLDIDDV
                     VGYLYSMSFASPAILGDRRPAFERDLRETLSALAGNGPFEEDHVLATYLAWKA"
     gene            2842..4092
                     /locus_tag="VFV93_10700"
     CDS             2842..4092
                     /locus_tag="VFV93_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX5165853.1"
                     /translation="MAEQISIDPAVVEERIMTLARIGACHETGVCRPTYTPEWIAAQA
                     LVEQWAQAAGMATRYDAVGDLYGRVEGSEGGKVILTGSHIDSQMPGGRYDGALGVISA
                     LTAIETLVRTYGQPRRPLEMISFCEEEGSAFSGARFWGSRAITSDIPDGEWNRIKGIY
                     GDMTGKPIGDVMRQLGYPPERIPDARRDDLDVFIELHVEQGPILEQQGLPVGIVNIIN
                     GTRGFQVTVQGVANHAGARPMDMRNDPLVAAAEMVLAVNRNALAMGRPAVSTVGHLVV
                     EPNGSAIIPDKVTFSIDCRHNDPVKLAELRAAHERSVTEIAAKHGVEVSWSQRPPLEP
                     CVCDPDVVATLEAAAREQGIPALTMPSGALHDTQRMSKVARVAMIFVQSRDGRSHTPA
                     EFTSIEHAVAGIQVLAAALHKLAY"
     gene            4310..5653
                     /locus_tag="VFV93_10705"
     CDS             4310..5653
                     /locus_tag="VFV93_10705"
                     /inference="COORDINATES: protein motif:HMM:NF015616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HEX5165854.1"
                     /translation="MRGAIRRGRWLLPVLVCLLALSGLPAGPLQDDDRASLYRSGADV
                     PLPDPIVPEPTATPTAERALLNIEGPTVTPQPAPPTPAATATPEAAAVSVPEADRIRA
                     RKRAIAIYSSLWEQFYLPDDRLFRETAPYDTGYPYAYLWPLSQTLGVATEMAALPEAD
                     PQYTFDALSVASAIESYFDDDRQPPAYSSYIPPPYGHANDRFYDDNAWIGIELMRAYD
                     VTGYELAIERAGQIFDYLVSGWDASESGPTSGGVYWVEASWSQHRNTVSTAPAAMLGL
                     LLAAQEDDAGRRHYELEWSKRMYDWVDATLRDPGGLYWDHIDENGVIDEAIYTYNQGA
                     MLGASLLLYQATNDERYLDRADEIGRAVIRRWDVDGLLAQPIAFNAILLANLLTLNDA
                     RPNQAYIDLFVAYADRLWDEQRDPDTNLVTASVPADLLNQSAAARIYAMLAGYGS"
     gene            5678..6229
                     /locus_tag="VFV93_10710"
     CDS             5678..6229
                     /locus_tag="VFV93_10710"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5165855.1"
                     /translation="MNQSDILRGERVRLTAMRPEDAATLARWWEQGAFLRQYDSVPAV
                     PRTEAQIAKDIATHQDRDDTYLLAIRRLDDDLLLGQLEFDGIAWTHRTTFFSIVIFDP
                     ELRGQGYGQEALELGLRFAFHELNLHRVALTVFAYNTRAITLDERLGFVHEGTHREFL
                     ERDGQRHDMLLYGMLRHEWEARR"
     gene            complement(6343..>6561)
                     /locus_tag="VFV93_10715"
     CDS             complement(6343..>6561)
                     /locus_tag="VFV93_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165856.1"
                     /translation="AFADGLARTADRPMARLTRSGGAVTRLDLWPEDDDIGRPVILPG
                     GEVGILRSWWNAPDGSEWRWTVELYNAR"
BASE COUNT         1146 a         2182 c         2149 g         1084 t
ORIGIN      
        1 ggcatgtacg ggatgttcgc gatcatcgag gccgtccgcc aggtgcgcgg cgactacgcc
       61 gaccagggca tccgtcaggt cccgaactgc gagctggccc tcgtccacgg cacgggtggc
      121 gtgctgtcgt ccgccgggac cgcgattctg gcgcggggat agtgattcgg gaaccgggat
      181 tcgggattcg ggttcattga ggaaagatcg tcctctggat agcccccgaa tcccggatcc
      241 cgaccaaaag gagacacaca tggccgatta caagaaacca ctgccgatgc cggacccggt
      301 cacgcagccg ttctgggata gcaccaaggc gcacgcgatg aagatccagc gctgcaacga
      361 caccggcaag ttcttcttct acccgcgcgg gctgagcccg ttcacgctct cggacaacat
      421 ctcgtgggag ccggtctccg gcaaggggac gctgcacgcc ttcacgatcg tccagaacca
      481 gcgccagcca ggtttcgcgg aggaggtgcc ttacgtcgtc gcgatggtcg agctggacga
      541 gggcgtccgg atgatgacca acctgatcgg cgtcgagccc gacccggcgc acgtcaagat
      601 cggcatgccg gtcgaggtcg tctacgagga cgtctctgag gagatcacgc tgccgaagtt
      661 caagccggca gggtaggctt ccactcagcc agcaaccagg ggccggtctc gtaccggccc
      721 tgtttcctgc ccgcacgaca caagaggaac caggagagcc ccggtggacc aggttattca
      781 agtagtcggc gcaatgatga ttctggcggg gtttgtcctg gcgcagttca aggtcctgcg
      841 ggtcgagtcg atgacctatc tcgtcctcaa cgtgatcggc tcggcgatcc tcgcctggct
      901 ggcgtgggat gaccggcagt ggggcttcct gctgctggaa ggcgtctggg cgatcgtctc
      961 ggcctggagc atcatccagt tgctgcgcgg gggcgatgta ccctcggcgc attgactaac
     1021 cccgttcgcg taggaacgat ggcaacgact acggatttcg tgccgccatc gccttgccga
     1081 cgtaggcaaa cagctcgcgg tcgatctgct gtccatccgg cagtgagtcc tcattctcga
     1141 agaggttcgt cagcgactgg gcgatgccgt cgttgccgcc ggtgaattcg gcgacgaggc
     1201 cgccccagtg atccagcagc ggctggagct tcgggtcggc agggtcggtg cctgcaacca
     1261 tctctgcgcg gacctcggcc atcaatcgcg gccactcggc ttcgacctca cggatgcgat
     1321 cctcgccaac ctcttcacgg cgcttgcgca gccactccag ttgctcttca gagtagtacg
     1381 tgttcaccat cgtcatcatc tccatcgctc gtagtagttc atcgaccggc gccgtcccat
     1441 tccggcgcag gtggccggcg atctgatcca actggcccag gagccgctgc tggagccgga
     1501 cctgatcgcg gacgcgcgcg atgtgcatct cgatcactgg caacgccgag cgatcccgac
     1561 cggccagcaa ctcacggatc tcgtccaacg gcaagccaaa ctggcgcaac gacgcgatct
     1621 gctgcagccg caggacgtcg gcgtcgctgt acagccggtg acccgcctca gttcgcgtcg
     1681 agggcgagag cagcccgatc tcgtcgtagt ggtgcagcgt ccgcaccgtc agcccggtcc
     1741 gccgcgccag ttcgccaacc ttcaactacc tgcacctcgc tgcctgcggt ccctggcgac
     1801 cacccagcca tcgtacaacc tcacgttacg tgaggtgcaa gtccccagtc gttctgagcc
     1861 gcagcgaagc atctttccgc gttgacgacc catgcgatca agtcagagga ggtctttcgc
     1921 tgcggctcag aacgactggg ggctcacgct ttccaggcca gatacgtcgc gagaacgtgg
     1981 tcttcctcga acgggccgtt accggcgagc gcggagagcg tttctcgcag gtcgcgttcg
     2041 aaggcggggc ggcggtcgcc gagaatggcc ggcgaggcaa acgacatcga gtacaggtag
     2101 ccgacgacgt cgtcgatatc gagccggcgg gtgtaggcga actcctggaa ctcgacctgg
     2161 gagaacggcg aacgagccag gaggtcgccc tcgctccagg ggatgcggtc ggatctggcc
     2221 gcgtaggtgc cgctgccggc ccggcgctcc tcgccgagcc agcgctgaat cacctcgcgg
     2281 acggcgacct gccagtcgcg ttggccgctc cagatctcgc acggctcgcc acattggaca
     2341 acaccgccac cggggaccgt cagcgcaccg agcgcggaca gcgtcgcatc gccatccatc
     2401 cagtgaaagg cgcgaccgat cgttgtcagc cgcagcggcg ccaactggtc gtgcaagcgt
     2461 tccaggtcga gcgagccgcc ctcgatccag acggcattgg tgatgccggc tgcctcggct
     2521 acccgctggg cttcgttcag catctccggc tcggggtcca tcccaatcgc cgtctcgacg
     2581 tgcggcgcga gcggaatgag cagttggccg gtcccgcagc cgaggtcgag cagccggccg
     2641 ctgccgtcga ggccgaagcg gtcgatgacg tgcgcgatca tcgcgtccgg gtagccgggg
     2701 cgatagcggg cgtaccaggc tgcggtccct ttgaacgagt cggtgtagcc ggacgacatg
     2761 gctggttcgc tcctttccag cgggtcaggc gctattatcc ttgttgggca gaattgacgg
     2821 cagcgggagg gcagggagcc gatggcggag cagatcagca ttgacccggc ggttgttgaa
     2881 gagcggatca tgacgttggc gcggatcggc gcctgccatg agaccggcgt ctgccggccg
     2941 acctacacgc cggagtggat cgccgctcag gcgttggtcg agcagtgggc gcaggcggcc
     3001 gggatggcaa cgcgctacga cgccgtcggc gacctctacg gacgcgtcga gggtagcgag
     3061 ggcggcaagg tgatcctgac cgggtcgcac attgactcgc agatgccggg cggacgctac
     3121 gacggcgcgc tgggggtcat ctcggcactg acggcgatcg agacgctggt acgcacctac
     3181 ggccagccca gacgtccact ggagatgatc tcgttctgtg aggaagaggg cagcgccttc
     3241 tccggcgcgc gcttctgggg atcgcgggcg atcaccagcg acatccccga cggtgagtgg
     3301 aaccggatca agggaatcta cggcgacatg accggcaagc cgatcggcga tgtcatgcgg
     3361 cagttgggct accctccgga gcggatcccg gacgcgcggc gtgacgacct ggatgtcttc
     3421 atcgagctgc acgtcgagca agggccgatt ctcgagcagc agggattgcc ggtcgggatc
     3481 gtcaacatca tcaacggcac gcgtggcttt caggtgacag tccagggcgt cgccaaccac
     3541 gccggggcac ggccgatgga catgcgcaac gacccgctcg tcgcggcggc cgagatggtg
     3601 ctggccgtca accgcaacgc gctggcgatg ggccggccgg ccgtctcgac cgtcggccac
     3661 ctggttgtcg agccgaacgg atcggccatc atccccgaca aggtgacgtt ctcgatcgac
     3721 tgccgccaca acgacccggt caaactggcc gagctgcgcg ccgcccacga gcggtccgtg
     3781 accgagatcg ccgcgaagca cggtgtcgag gtctcctggt cgcagcggcc gccgctcgaa
     3841 ccatgcgtct gcgatccgga tgtcgtcgcg acgctcgaag ccgcggcccg cgagcagggc
     3901 atcccggccc tgacgatgcc gagcggggcg ctgcacgaca cgcagcgcat gtcgaaggtg
     3961 gcgcgggtcg cgatgatctt cgtccagagc cgcgacggac gcagccacac gccggccgag
     4021 ttcacctcga tcgagcacgc cgtggccggc atccaggtgc tggcggcggc gttgcacaag
     4081 ttggcgtatt gacagggtag ccgatacatt cgtcccgttt gtcatcccga cgcccccttt
     4141 gtcatcccga cgaaggaggg atttctggcc gaagacgcga ggatcctcaa gagccgcaca
     4201 gttctgacca gagatccctc cttcgtcggg atgacaaagg gggcgcagac ggttgcaggg
     4261 ccgccgtgtt tgtgcgatat cttctcccat gcaacaacga cgacgcggga tgcgtggggc
     4321 cattcgtcgt gggcgctggc tgctgccggt gctcgtctgc ctgcttgccc tcagcggatt
     4381 gccggctggg ccacttcagg acgacgaccg cgccagcctc taccgcagcg gcgcggacgt
     4441 cccgctgccg gatccgatcg tgccggagcc gacggcgacc ccaaccgccg agcgtgccct
     4501 gctcaacatc gaaggcccga ctgttacccc gcaaccggca ccaccgacgc cggcagcaac
     4561 tgcgactccg gaggcggcag ccgtgagcgt gcctgaggcc gatcgcatac gggcgcggaa
     4621 gcgggccatc gcgatctaca gctccctctg ggagcagttc tacctgccgg acgaccggtt
     4681 gttccgcgag acggcgccgt atgacacggg ctatccctac gcgtatctct ggccgctgtc
     4741 tcagacgctc ggcgtcgcga ccgagatggc ggccttgccg gaggctgacc cgcagtacac
     4801 cttcgatgca ctgtcggtcg ccagtgcgat cgagtcgtac tttgacgacg accgccagcc
     4861 gccagcctat tcgtcgtaca tcccgccgcc atacggccac gcgaacgacc gcttctacga
     4921 cgacaatgcc tggatcggca tcgagctgat gcgcgcctat gatgtgaccg gctatgagct
     4981 tgcaatcgag cgggccggac aaatcttcga ctacctcgtc tccggctggg acgccagcga
     5041 gtccggccca acctccggcg gcgtctactg ggtcgaagca tcctggagcc agcaccgcaa
     5101 cacggtcagc acggcaccgg ccgcgatgct cggactgctg ctggccgctc aggaagacga
     5161 tgcgggccgc cgccactacg aactcgagtg gtcaaagcgg atgtacgact gggttgacgc
     5221 gaccctgcgc gatcccggcg gactctactg ggaccacatc gacgaaaacg gcgtcatcga
     5281 cgaggcgatc tacacctaca atcaaggggc gatgctcggc gccagcctgc tgctctacca
     5341 ggcgaccaac gacgagcgct acctcgatcg ggccgacgag attggtcggg cggtgattcg
     5401 gcgctgggat gtcgacggcc tgctggccca gccaatcgcc ttcaacgcga tcctgctggc
     5461 caacctgctg acgctcaacg acgcgcggcc caaccaggcc tacatcgacc tgttcgtcgc
     5521 ctacgcagac cggctctggg acgagcaacg cgatccggac accaacctcg tcacggccag
     5581 cgtcccggct gatctgctca accagtcggc ggccgcccgg atctatgcga tgcttgccgg
     5641 ctacgggagc tagccagtcc ggcaaggagc aatcggtatg aaccagagtg acatcctgcg
     5701 cggcgagcgc gttcggctga cggcgatgcg gcccgaggac gccgcgacgc tggcacgctg
     5761 gtgggagcag ggcgcgttcc tgcgccagta cgactcagtc ccggctgtcc cgcgcacgga
     5821 ggcgcagatc gcgaaggaca tagccaccca ccaggaccgc gacgacacgt acctgctggc
     5881 gatccgccgc cttgacgacg acctgctgtt gggccagctt gagttcgacg gcatcgcctg
     5941 gacgcaccgg acgaccttct tcagcatcgt catcttcgat ccggagctac gcggccaggg
     6001 ctacggccag gaggcgctgg agctcggcct gcgcttcgcc ttccacgagc tcaacctgca
     6061 ccgggttgcg ctgaccgtct tcgcctacaa cacccgcgcg atcaccctcg acgagcggct
     6121 cggcttcgtc cacgaaggca cccaccgcga atttctcgaa cgcgacggcc agcgccacga
     6181 catgctgctg tacgggatgc tgcggcatga gtgggaagcg cggaggtgac aggtgataga
     6241 tgttaggtgt taggagcaga tccgtgggag cggaagcgtt acgttgatcc gcacagtcca
     6301 tcccctaaca cctatcccct atcacctatc ccctatcacc tgtcacctcg cgttatacag
     6361 ctccaccgtc cagcgccatt cgctaccgtc tggggcgttc caccagctcc tgaggatgcc
     6421 aacctcgcca ccggggagga tgacggggcg gccgatgtca tcgtcttcgg gccagaggtc
     6481 gaggcgggtg accgcgccac cggagcgggt gagccgggcc atcgggcggt cggccgttcg
     6541 ggcgagcccg tccgcgaagg c
//
LOCUS       DASUGJ010000479         4516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1186315, whole genome shotgun sequence.
ACCESSION   DASUGJ010000479 DASUGJ010000000
VERSION     DASUGJ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186315"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFV93_10720"
     CDS             complement(<1..339)
                     /locus_tag="VFV93_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX5165857.1"
                     /translation="MRILVTGATGSIGRLVVDRLMESGTAQVRALTTNPAKAALPDGV
                     EVVRGYVGKPETLGTAFDGVDKVYLAPVPGAVAEVVKRACDAGVQPIVDLAGAPYTSW
                     NVVSVAVESSG"
     gene            692..1612
                     /locus_tag="VFV93_10725"
     CDS             692..1612
                     /locus_tag="VFV93_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5165858.1"
                     /translation="MSQVNGAVPSLDTVEDVLQSLGTQRYVAETSLATAIFLGLKLPK
                     PLLLEGEAGVGKTEIAKVLSAILQTDLIRLQCYEGLDVSHAVYEWNYPRQMLAIRAAE
                     SSHESREAAIAQIFSEDYLIKRPLLQAIEHRGKAPVLLIDEIDRADEEFEAFLLELLS
                     DFQVTVPELGTMKAEYPPIVIITSNRTRELHDALKRRCLYHWIDYPTIEKEYKIVQTK
                     VPGINDRLAGQISHFVHDLRDIDLYKLPGVAETLDWAAALMALNQRELSAEVASSTLG
                     AILKHQEDIQSVRKNKLDDLVAAAASNGLS"
     gene            1614..2909
                     /locus_tag="VFV93_10730"
     CDS             1614..2909
                     /locus_tag="VFV93_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5165859.1"
                     /translation="MAAQPHVHQPSEQGQKLLRRSLQFGRLLRANGIKVTSSQVYDFV
                     HATEHIGVRDRRVFKEAAEACMVTHGEQREMFSLLFDRYWRAKRIVDESAEQMPNFAD
                     QSAEMIEGLDDAEGEEGEGQGQMAEGMEGLAMDEDSDGGDIGEGEDSELESVLTYSGS
                     EALRSKDFSEFTQEELEQAKILMKHLKWEIGMRKSRRKVASSKGRFIDARKTMRKSLQ
                     TAGVPLKISERKIKMKPRSLIVICDISGSMDRYSRLLLQFIHTIENDMAKVEAFVFGT
                     RLTRITRILKKRPIDEAITRVSREVQDWAGGTRIGESIQAFNTEYARRVLRNGAVVLI
                     ISDGWDRGDPQLLGTEMMRLQRSCYRLIWLNPLLGSPRYQPLTRGMQAALPFIDDFMP
                     VHNLESLELLADHLSKVGEFRPVRKQAPLEFVNQRMAAV"
     gene            3035..3529
                     /locus_tag="VFV93_10735"
     CDS             3035..3529
                     /locus_tag="VFV93_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX5165860.1"
                     /translation="MALMDHLLHANQLYSETFRMGRLPGSPRLELAILTCMDSRIDPD
                     RILGLQEGDAHVIRNAGGRASDDAIRSLVISYRLLGTREFLVIHHTDCGMRKITNEQM
                     HAELLHDLHADVSDVDFMPISDPDETVRADVDAIRTSPMVRADIQVHGFVYDVATGRL
                     RSVV"
     gene            3765..4091
                     /locus_tag="VFV93_10740"
     CDS             3765..4091
                     /locus_tag="VFV93_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX5165861.1"
                     /translation="MRDVLEYIDEWTSEGEQVAVATVVSAAGSTPRPVGAKLVVSKSG
                     RMQGSVSGGCVEGDVFRTAMEVMESGMPQLVHYGISDEMGWEVGLSCGGTIDVFVEPV
                     NANGTA"
     gene            4095..>4516
                     /locus_tag="VFV93_10745"
     CDS             4095..>4516
                     /locus_tag="VFV93_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX5165862.1"
                     /translation="MDSTVFSALRDAIQNEIPASNAKVVSGPDTGNELLIVYPDQVLG
                     GLGSPAADEQASRTLRAMLRDGGTTLDEIDGRQVFMEAHLPPPHLVIVGAVHIAIPLI
                     TFGQTLGYRTTVIDARAQFATDERFPHADRLIEAWPDEA"
BASE COUNT          858 a         1557 c         1316 g          785 t
ORIGIN      
        1 accggacgat tcgaccgcca ccgaaacgac attccagctc gtatatggcg ctccggccag
       61 atcgacgatg ggctgaacac cggcgtcaca ggcacgcttc acgacctcag caacagcgcc
      121 aggaaccggc gcgagataga ccttgtcgac tccgtcaaac gccgtcccga gtgtctccgg
      181 cttgccgaca tagccccgga cgacctcgac tccatcaggc agagccgcct tcgccggatt
      241 cgttgtcagc gccctgacct gcgccgttcc cgactccatc agacgatcga cgaccaaccg
      301 accaatgctt cccgtcgctc ctgtgaccag gattcgcatc tgtccatcct tatccgttct
      361 cacatgtcag cttcgtcgaa tccacagtgg ctctatctgg agcgaacaag cgtaacaaat
      421 gaacagtcca cttcacgtgc cctgcctgta gatgggcaac atatgccggc ccgatccgct
      481 atcccgttcc cttgtcccgc atcccgtttc ccgcgtcccc cgtctctcat gtcccatcgc
      541 ccatgcccaa tccatcgcca taccatcata tccgcgccga ttccagccca tctcgtgcta
      601 aacttcacgg ataccggcct gagggtttgt cgctcgcgaa cgatccgaat gaatcggcca
      661 cgatgcgagc gtgagagtga gggatgtttc gttgtcgcag gttaatggtg cggtgccgtc
      721 gctcgacacc gttgaagacg ttctgcaatc gctcggcacc cagcgctatg tcgctgagac
      781 cagcctcgct accgctatct tcctcgggct gaagctgccg aagccgctcc tgctcgaagg
      841 agaggccggc gtcggtaaga ccgagatcgc caaagtcctt tcggcgatcc tccagaccga
      901 cctgattcgc ctccagtgct acgagggcct ggatgtctcg cacgccgtct acgagtggaa
      961 ctacccgcgc cagatgctcg ccatccgcgc ggccgagtca tcccacgaga gccgcgaggc
     1021 cgccatcgcc cagatcttta gcgaggacta cctgatcaag cgcccgctcc tccaggcgat
     1081 cgagcatcgc ggcaaggcgc cggtcctcct gatcgacgag atcgaccgcg ccgacgagga
     1141 gttcgaggcc ttcctgctgg agctgctctc cgacttccag gtcaccgttc cggagctcgg
     1201 cacgatgaag gccgagtacc cgccgatcgt catcatcacc tcgaaccgga cccgcgagct
     1261 gcacgacgcc ctcaagcgcc gctgcctcta ccactggatc gactacccga cgatcgagaa
     1321 ggaatacaag atcgtccaga cgaaggtgcc gggcatcaac gaccgccttg ccggtcagat
     1381 ctcccacttc gtccacgatc tgcgtgacat cgacctctac aagctgccgg gcgtcgccga
     1441 gacgctcgac tgggccgccg ccctgatggc gctcaaccag cgcgagctgt cggccgaggt
     1501 cgcctccagc acgctcggcg caatcctgaa gcaccaggag gacatccagt cggttcgcaa
     1561 gaacaagctg gacgacctcg tcgccgcggc ggcaagcaac gggttgagct aacatggcag
     1621 cgcagccaca cgttcaccag ccgagcgagc aggggcagaa gctcctgcgg cgctcgctcc
     1681 agttcggccg gctcctgcgc gccaacggca tcaaggtcac ctcgtcgcag gtctacgact
     1741 tcgtacacgc gactgagcac atcggcgttc gtgaccggcg tgtcttcaag gaggccgccg
     1801 aagcctgcat ggtcacgcac ggcgagcagc gtgagatgtt ctcgctgctg ttcgaccgct
     1861 actggcgcgc caagcggatc gtcgatgaat ctgccgagca gatgccgaac ttcgccgacc
     1921 agtccgccga gatgatcgag gggctggacg acgccgaggg cgaggagggc gaaggccagg
     1981 gccagatggc cgagggcatg gaaggcctcg cgatggacga ggactccgac ggcggcgata
     2041 tcggcgaggg cgaggattcc gagctggagt cggttctcac ctacagcggc tccgaggcgc
     2101 tgcgcagcaa ggacttctcc gagttcaccc aggaggagct tgagcaggcc aagattctga
     2161 tgaagcacct caagtgggaa atcggtatgc gcaagtcccg ccgcaaggtc gcctcctcga
     2221 agggccgctt catcgacgcc cgcaagacga tgcgtaagtc gctccagaca gccggtgtcc
     2281 cgcttaagat cagcgagcgc aagatcaaga tgaagccgcg ctccctgatc gtgatctgcg
     2341 acatctccgg ctcgatggat cgctactcgc ggctcctgct gcagttcatc catacgatcg
     2401 agaacgacat ggccaaggtc gaagccttcg tcttcggcac gcggctgacg cgcatcacgc
     2461 gtatcctcaa gaagcgtccg attgacgaag ccatcacccg cgtctcgcgt gaggtgcagg
     2521 actgggccgg cgggacccgg atcggtgagt cgatccaggc cttcaacacc gagtacgccc
     2581 gccgcgtcct gcgcaatggc gccgtcgtcc tgatcatctc cgatggctgg gatcgcggcg
     2641 acccgcaact gctcgggacc gagatgatgc ggctccagcg ctcctgctac cggctgatct
     2701 ggctcaaccc gctgctcggc tcgccccgct accagccgct gacgcgcggc atgcaggcgg
     2761 cgctaccgtt catcgacgac ttcatgccgg ttcacaacct cgaaagcctg gagctactgg
     2821 ctgaccacct ctcgaaggtc ggcgagttcc gacctgtccg caagcaggcg ccgctcgaat
     2881 tcgtcaacca gcggatggca gcggtctagt atctactcgc gaacaccaat gggggcggca
     2941 attgccgtcc ccatgttcta ttctctccgc gtaggggcga tggccgcagc ctcgccccta
     3001 cgcgaggaga tcgtttctct cataggaggc accaatggcc ctgatggatc acctgctgca
     3061 cgcgaaccag ctctactccg agacgttcag aatgggccgc ctccctggtt caccccgcct
     3121 ggaactggcg atcctgacct gtatggactc gcggatcgac cctgaccgca tcctcggcct
     3181 ccaggagggc gatgcccacg tcatccgcaa cgccggcggc cgcgccagtg atgatgcgat
     3241 ccgctcgctg gtcatctctt accgcctgct cggcactcgc gagtttctcg tcatccacca
     3301 caccgactgc ggcatgcgca agatcaccaa cgaacagatg cacgcggaac tactgcatga
     3361 tctccacgcc gatgtctccg atgtcgattt catgcccatc agcgaccccg acgaaaccgt
     3421 ccgcgccgac gtcgacgcca tccgcacctc cccgatggtc cgcgccgaca tccaggtcca
     3481 cggcttcgtc tacgatgtcg ccactggccg cctgcgatcg gtcgtctgac gaccgtcctt
     3541 tgtcgtcccc aacagcacac cacaaaggct gcgccacgtt cctccctttg tcatcccgag
     3601 gcgtgagccg aagcatctct cctcggatga ctgatcggcc aacgcaggac agatccttcg
     3661 gctcacgcct cacgatgaca aagttgtggg gaatgtccca cgctggcggg acgctataca
     3721 atggcagagg gaacacaccg cacccacgac aggagcacca aggtatgcgc gacgtactcg
     3781 aatacatcga tgagtggacc agcgaaggcg agcaggtcgc cgttgcgacc gttgtctcgg
     3841 cggcgggatc gacaccacgg ccggttgggg cgaagctggt cgtctcgaag tccggcagga
     3901 tgcagggctc cgtctccggg ggctgcgtcg agggcgatgt cttccggact gcgatggaag
     3961 tcatggaatc cggtatgcca cagctcgtcc actatggtat ctccgacgag atgggctggg
     4021 aggtcggcct ctcctgcggc ggcacgatcg atgtcttcgt cgagccggtc aacgcgaacg
     4081 gcacggcctg agcgatggat tcgaccgtct tctcggcgct ccgcgatgcc attcagaacg
     4141 agataccggc ctcaaacgcc aaggtcgtca gcggcccgga taccggcaac gagctgctga
     4201 tcgtctaccc ggaccaggtg cttggcgggc ttggttcgcc ggccgccgac gagcaggcat
     4261 cgcgaacact acgagcgatg ctgcgcgacg gcgggacgac gctcgacgag attgatggcc
     4321 ggcaggtctt catggaagcg cacctgccgc ccccacacct cgtcatcgtc ggcgccgtcc
     4381 acatcgcgat cccgctcatc acctttggcc agacgctcgg ctaccggacg accgtcatcg
     4441 acgcccgcgc ccagttcgcg accgacgagc gcttcccgca cgccgaccgg ctgatcgagg
     4501 cctggccgga cgaggc
//
LOCUS       DASUGJ010000480         5985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_493225, whole genome shotgun sequence.
ACCESSION   DASUGJ010000480 DASUGJ010000000
VERSION     DASUGJ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493225"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VFV93_10750"
     CDS             complement(<1..930)
                     /locus_tag="VFV93_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165863.1"
                     /translation="MQVRERGTMVGAEATNAPVVPGPGTSQPAPAGKREVDLYARTYA
                     TLLRSSGPIAVDALVAAHLNIQSSLHANAADPAPDMNAFMYSTLRLPPEIVRVSRILL
                     GQSPTVFNRNGYTDLASWALVSAPGRRRRWHYDGHETLAVYVGSMSDLDDLVPTIVAW
                     QIEWNKLHVRIRDDEELKARVVAAGRHVEPEESEALAAALGIAASDWARLQLAWGAEF
                     WPNLHSIATAKRDLHLHMLGGTHLGFSRSARRWWGPVGRVLAERGLSDRPVYFVSSNL
                     HSIANILSGTARRRRDELIAFIEDSQHPDLLPEL"
     gene            1150..2415
                     /locus_tag="VFV93_10755"
     CDS             1150..2415
                     /locus_tag="VFV93_10755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165864.1"
                     /translation="MDDGVQDNPWRRNLYVLWVGETLTILGFTMCYPFLPFFLGELGA
                     TSFDSQALWTGAMMASSAGFMMITAPFWGMAADHFGRKPMIVRAMVCGAITTGLMGLV
                     VAPWQLLILFILDGALSGTVTAAMTLVATSTPKERLGYALGLLQTAIFVGLSAGPMVG
                     GVLADQVGYRPVFGIGGLLLAVAAALVLKLTRETFVRRPRAALDDNPQAHDTRLAAIL
                     LVGVLPAAVGITFALRLATGAITPILPLFVEELADAGAPIASLTGLTFGISGVGSALS
                     ALTLGRAADRLGHRRVLTASGLAVTLLFVPLALVQTPWQLMACYGVLGIATGGIVPSA
                     NALVADLTPVERRGAVFGVTSAVTSFGGFIGPFSGSLLAARADLRAVFLVVGAVMLAF
                     TIWLVHALRPRPLGASETVDDVESVSAAP"
     gene            2627..3025
                     /locus_tag="VFV93_10760"
     CDS             2627..3025
                     /locus_tag="VFV93_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5165865.1"
                     /translation="MQNNYPAQQRILIVEDEADIRSLMSFVLERAGYDAVCVAAINEA
                     RAAISLYTFDAALVDLYLPDGRGDEVVGLLRSESPSTRIVVTSAFTHSPLAPRFETEA
                     GDTFLPKPFNPAALIAAVASRPAGTIVASA"
     gene            3296..4795
                     /locus_tag="VFV93_10765"
     CDS             3296..4795
                     /locus_tag="VFV93_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX5165866.1"
                     /translation="MRVAIGGIAHETNTFSSIPTDLARFQQRSLLRGDALLRASRGVS
                     NVLGGMVDTATALGWEIVPLLFASATPSGKVRRGTFETLANDLERGLADAIRKQPLDG
                     VMLALHGAMVAEAVGDADGELLRRVRRAVGPETPIVAVFDFHANLTPKIVAHADLILG
                     FETYPHIDTDAQGSQAVRLLEQLTRGAFRPVHALHQVPMLLTLPAQATSPGSPMDELM
                     QLAERLKQADGIVNIVIAGGFPYSDIRDAGLAVVVTTDDNPALAERIAGQIARAAWER
                     RERFRPELTSIVEAIEIARSGGNESGPVVLADVADNPGAGGSGDGTAILAALLEAGVD
                     GAALAVITDPEAVDRAWQTGQGNRGQFRLGGKIDRKHGPTLEMEARVRLTGEVAFTNR
                     GPMGAGSRSRLGRTAVLEVRGAGGVTVEVIVCEYRMQVLEPEVFRACGIAPEQRRVLG
                     VKSSVHYRAAFAPLAGRLVDVDGPGLASPTLANFHYRRLRRPIWPLDEV"
     gene            5034..5912
                     /locus_tag="VFV93_10770"
     CDS             5034..5912
                     /locus_tag="VFV93_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5165867.1"
                     /translation="MASQPQTMILLIEDHKEFQELVTALLEAEGFVVVAAGTSAAALE
                     ALDTLRPALILLDSSLPDGDGAQLARAIRARRIGAPIVLMTTDPRSAGLATEIGAVEV
                     LVKPFSTASLLATVRNRLSNAAPIDLNIQTGQMDAIAADMISPTRDLGDAPPSRQPAQ
                     GSQPAPNAPVTPPPATTDQWEYCQLIAAGRGEIRGETYYSVDLVFMGSTVTARRIASR
                     EGPAAAPWTTNPWKQAIGMLGLAGWEMVNIQHGDYAGRAAENSTLSWNTVVAYFRRPV
                     LPNRPITEPKISLTRT"
BASE COUNT          966 a         1990 c         2015 g         1014 t
ORIGIN      
        1 cagctccggc agcaggtccg ggtgctggct gtcctcgatg aaggcgatca gctcgtcgcg
       61 acggcgacgg gcagtgccgc tcaggatgtt ggcgattgag tgcagattcg aggagacgaa
      121 atagacgggg cgatcggaca atccccgctc ggccagcaca cgaccgaccg gcccccacca
      181 gcggcgggcc gagcgcgaga agcccagatg cgtcccgccg agcatgtgca ggtgcaggtc
      241 gcgtttggcc gtcgcgatgc tgtgcaggtt gggccagaac tcggcgcccc aggcaagctg
      301 gagtcgcgcc cagtcactgg cagcgatccc caacgccgcc gccagcgcct ccgactcctc
      361 cggctcgacg tgccggccgg cggcgacgac ccgtgccttc aattcctcgt cgtcgcggat
      421 gcgaacgtgc agcttgttcc actcgatctg ccaggcaacg atcgttggaa cgaggtcgtc
      481 gaggtcggac atcgagccga cgtagacggc cagcgtctca tgcccgtcgt aatgccagcg
      541 gcgacggcgg ccgggcgccg agaccagagc ccagctggcg agatcggtgt agccattgcg
      601 gttgaagacg gtcggcgact gacctagcag aatccggctg acacggacga tctccggcgg
      661 cagccgcagc gtgctgtaca tgaaggcgtt catatcgggc gcggggtcgg cggcgttggc
      721 gtgcaggctg gactgaatgt tgaggtgggc cgcgaccagg gcatcgacgg cgatcggccc
      781 agacgagcgc agcagcgtcg cgtacgtccg cgcgtagaga tcgacctcgc gcttgccggc
      841 cggtgcgggc tggctggtac cgggtcccgg cacgaccggc gcgttcgtcg cctccgcgcc
      901 taccatcgtc ccacgttcgc gcacctgcat ggcactgccc ccttcgcaca tctccggacg
      961 gccgccacag gccgtagtgg atgatagcag gctgccagct acccgtgagg tggtcgtgtt
     1021 tcaggtagct ggtctcatcc gcgtcgtggc ggaggcgtcc gcgcgttcac gaactcgtgt
     1081 tgccacctgc aatgtgcgaa catagctgat cgtcaccaac ggaacggcca gccggaagca
     1141 gcgcgcaggg tggacgacgg cgtacaagac aacccgtggc ggcgtaatct gtacgtgttg
     1201 tgggtcgggg aaacgctgac gatcctcggc ttcacgatgt gctacccatt cctaccgttc
     1261 ttcctgggcg agcttggagc gacatcgttc gacagccagg cgctctggac cggcgcgatg
     1321 atggcgagca gcgccggctt catgatgatc acggcgccgt tctgggggat ggccgccgac
     1381 cacttcgggc gcaagccgat gatcgtccgc gcgatggtgt gcggtgcgat cacgaccggg
     1441 ctgatgggcc tcgtcgtcgc gccgtggcaa ttgctgatcc tgttcatcct cgacggcgcg
     1501 ctttccggca cggttacggc agcgatgacg cttgtcgcca cctcgacgcc gaaggagcga
     1561 ctcggctacg cgctggggct gttgcagaca gcgatcttcg tcggcctgtc ggccgggccg
     1621 atggtcggtg gcgtgctggc cgaccaggtc ggctaccggc ccgtcttcgg gattggcggc
     1681 ctgctgctcg ccgtcgccgc cgcgctcgtg ctgaagctga cccgcgagac attcgtccgc
     1741 cggccgcgtg ccgcgcttga cgacaacccg caggcgcacg acacacgtct ggccgcgatt
     1801 ctgctggttg gcgtgctgcc ggcggcggtc ggcatcacgt tcgcattacg gctggcaacg
     1861 ggcgcgatca cgccgatcct gccgctgttt gtcgaagaac tggctgacgc cggcgcgccg
     1921 atcgcgtcat tgaccgggtt gacgttcggc atctccggtg tcggcagtgc gttgtcggcg
     1981 ctgacactgg ggcgcgcggc cgaccggcta ggccaccggc gcgtgctgac cgcctcggga
     2041 ctggcagtga cgctgctctt cgtcccgctc gcgctggttc agacgccctg gcaattgatg
     2101 gcttgctacg gggtactggg catcgcgacg ggtgggatcg tcccgagcgc caacgcgctc
     2161 gtggccgacc tgacgcccgt cgagcggcgc ggcgccgtct tcggcgtgac gagtgcggta
     2221 acgtcgttcg gcggtttcat cggcccattc agtggctcgc tgctggcggc cagagccgat
     2281 ttgcgcgccg tcttcctcgt cgtcggcgcc gtgatgctgg ccttcacgat ctggctggtc
     2341 cacgcactgc gcccgcgacc gctcggagcg tccgaaaccg tggacgatgt cgagagcgtt
     2401 tcagcggcgc cctgatagcc agtcgcaacc acgccacacg aagcacactc gcccgccttc
     2461 ctacccaacc acgccatcac gcgacggtac tttcattgaa agaaacgaaa cgccgtatcg
     2521 agtgccagcc catctgtgac actcctgtga tacgaaatgt cccccagagc cgcgttgagc
     2581 gattagtgtt ctagcacgcg tcgcaaccgc gtctatggga gtcatgatgc agaacaacta
     2641 tcccgcacag cagcgcattc tgattgtcga agatgaggcg gatatccgct cgttgatgtc
     2701 gtttgtgctt gagcgcgctg gctacgacgc cgtctgtgtc gcggctatca atgaggcgcg
     2761 cgcggccatc tcgctctaca cgttcgacgc ggcgctggtg gatctctacc tgccggacgg
     2821 ccgtggcgac gaggtggttg gtctcctgcg gagcgagtca ccctctaccc gcatcgtcgt
     2881 gacctcggcc ttcacccact ctccgctggc gccgcgcttc gagaccgagg ccggtgacac
     2941 gttcctgccg aagccattca acccggcggc actgatcgcg gccgtcgcca gccgaccggc
     3001 aggaaccatc gtcgcaagcg cctgagcgcg ccagcgtgtc catcgttcat tcctctccat
     3061 cctccctctc ggacccgggc ctgcagtccc gggttcgagc atgttcggcg ggccaattgc
     3121 gccggggacg gggactcggc aatactcgcg cggcgtggat gccggggatc gggaatgagg
     3181 gaactgggat gaggcagcag cgggacaatc ggcggcggcc atcggttcac accggcaacg
     3241 accggccacc ggttcgcatc tccaggaatc cagcccgact cactcgcggt tcgtcatgcg
     3301 cgtagcgatc ggcggcatcg cccacgagac gaacacgttc tcgtcgatcc cgaccgatct
     3361 ggcgcgcttt cagcagcggt cgctgctgcg tggcgatgcg ctgctgcggg ccagccgggg
     3421 cgtcagcaac gtcctcggcg ggatggtcga cacagcgacg gcgctcggct gggagatcgt
     3481 gccgctgttg ttcgcgtcgg cgacaccgtc cggaaaggtc cggcgcggga cgttcgagac
     3541 actggccaac gatctggagc gcggactggc agacgccatc cgcaaacagc cgctcgacgg
     3601 ggtgatgctg gcgctgcatg gcgcgatggt cgccgaagcg gttggcgatg ctgacggcga
     3661 actgctgcgc cgcgtccggc gagcagtcgg gccggagaca ccgatcgtgg ccgtcttcga
     3721 tttccacgct aacctgacgc cgaagatcgt tgcccacgcc gacctgattc tcggctttga
     3781 gacctacccg catatcgaca ccgacgccca ggggagccag gctgtccggc tgctggaaca
     3841 gctcacacgt ggcgcgtttc gtcccgtcca cgccctgcac caggtgccga tgctgctgac
     3901 gctgccggcg caagcgacca gccccggatc gccgatggac gagctcatgc aactggcgga
     3961 gcggctcaag caggcggatg ggatcgtcaa catcgtcatc gccggtggct ttccctacag
     4021 cgacattcgt gatgccgggc tggccgtcgt cgtcacaacc gacgacaacc cagccctggc
     4081 cgagcggatc gccgggcaga ttgcccgtgc cgcctgggag cgtcgcgagc ggttccgccc
     4141 ggaactgaca tccattgtcg aggcgatcga gatcgccagg tccggcggca acgaatccgg
     4201 gccggttgtc ctggccgatg tcgccgacaa tccgggtgcc ggcggctccg gcgacggtac
     4261 ggcgattctg gcggccctgc tggaggccgg tgtcgacggc gcggcgctgg cggtgatcac
     4321 cgatccagag gccgttgatc gtgcctggca gactggccaa ggcaaccgtg gccaatttcg
     4381 gttgggtggc aagatcgacc ggaagcacgg accgacgctt gagatggagg cgcgggttcg
     4441 cctgaccggc gaggtagcgt tcaccaatcg cggtccgatg ggcgccggat cgcgcagccg
     4501 gctgggacgg accgccgttc tggaggtgcg tggggccggc ggcgtgacgg tcgaggtcat
     4561 cgtctgcgag tatcgcatgc aggtgctgga gccggaagtg ttccgtgcct gtgggatcgc
     4621 ccctgagcag cgccgggtac tgggcgtcaa atcctcggtc cactaccggg cggccttcgc
     4681 gccgttggcc ggcaggctgg tcgatgtcga tggccccggc ctcgcctccc caactctggc
     4741 caacttccac taccgccgcc tccgccgccc gatctggccg ttggacgagg tgtgacgagg
     4801 tggtaggtga tagctgttaa gtgttagggg ctggtccgtg cgggaggaac ctgtcacgct
     4861 gatcccacac gatcgagccc ctaatcccta tcacctgata cctgatgcct caacttgcac
     4921 aagtcagcac aacaagcgat gattacaggc atcatgtgca ggcgagggag tggctgggca
     4981 gcgtggctcg gcaccggcat tgcgagtcgc gaatcggcgc gcgaggatga gacatggcca
     5041 gtcaaccgca gacgatgatt ctgctgatcg aagaccacaa ggagtttcag gagctggtga
     5101 cggccttgct ggaggccgag ggcttcgtcg tggtcgccgc cggaacgagc gctgccgcgc
     5161 tggaagccct cgatacactc cggccggcgc tgatcctgct ggacagctcg ctgccggatg
     5221 gcgatggcgc ccaactcgcg cgggcgattc gggccagacg gatcggcgcg ccgatcgtcc
     5281 tgatgacgac cgatccgcgc tcggccggcc tcgcaaccga gatcggggcc gtcgaagtgc
     5341 tggtcaagcc cttctcgacc gcctccctcc tcgcaaccgt ccgcaaccgc ctcagcaacg
     5401 ccgccccgat cgacctcaat atccagacgg gacagatgga cgccatcgcg gccgacatga
     5461 tctccccaac ccgcgacctc ggtgacgcgc cacccagccg gcaaccggcg caggggtcgc
     5521 aaccggcgcc gaacgcgccg gtcacgccac ctccagcgac gactgatcaa tgggagtatt
     5581 gccagctgat cgcggccggc cgaggcgaaa tacgcgggga aacctactac agcgtggatc
     5641 tggtcttcat gggttcgacg gtgaccgccc gccggatcgc cagccgcgag ggaccggccg
     5701 ccgcgccctg gacgaccaac ccgtggaaac aggcgatcgg gatgctcggt ctggccggct
     5761 gggagatggt caacatccag cacggcgact acgccggccg ggccgccgag aactcgaccc
     5821 tctcctggaa caccgtcgtc gcctacttcc ggcgtccggt tctgcccaac cgcccgatca
     5881 ctgagccgaa gatcagcctg acccgaacat agcctgtcgt cctgaacggt tgacagggtg
     5941 aatgcgacac caaccctgtg taggggccgt gcctccgtgc cggcc
//
LOCUS       DASUGJ010000481         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_493410, whole genome shotgun sequence.
ACCESSION   DASUGJ010000481 DASUGJ010000000
VERSION     DASUGJ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493410"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..502)
                     /locus_tag="VFV93_10775"
     CDS             complement(44..502)
                     /locus_tag="VFV93_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5165868.1"
                     /translation="MQVEQRLKELGLELPDAPAPIANYLRYVQVGNLLFMAGHGPNRS
                     GGPVYAGKVGADVSLEEAYEAARSTTLNLLASAKEALGDLDRIKKVVKVLGMVNSAPG
                     FGDQPKVINGFSDLMVELFGENGRHARSAVGMAALPGQIPVEIEMILEVE"
     gene            complement(588..923)
                     /locus_tag="VFV93_10780"
     CDS             complement(588..923)
                     /locus_tag="VFV93_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165869.1"
                     /translation="MTTEASRSEIDKLLIRQIGRWAVVLTILLALFDVAMLRSWPFPD
                     AIEAARGAFYVIVAVALLLGTLLVTFLGYRIVTMRVATRARSVYLIFLPGVFLLSTIV
                     GFLLLTAPR"
     gene            complement(920..2602)
                     /gene="ade"
                     /locus_tag="VFV93_10785"
     CDS             complement(920..2602)
                     /gene="ade"
                     /locus_tag="VFV93_10785"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEX5165870.1"
                     /translation="MLEHWQRALRIATGGEAADLLLRGGQVVNVFTGEVEHGTIAIAD
                     GRIVGIGDYTAAERVVDLDGAFVAPSFIDGHFHTESSLLWITQFARTVVPHGTGAIVT
                     DPHEIVNVAGLSGFNALVDASRDLPLAVYFTVPSCVPASPRETAGAVISAGDIAAGLQ
                     NPAAVGLGEMMNFPGVLGGDPEIFARLEAANGWRKDGHAPGLTGRALDGYALSGMTSD
                     HESTSLEEGREKLRRGMMLMIREGSTEHNLRELLPLVNDRTWARCCFASDDRDCTTLL
                     HDGHMNQTLRLAVSEGLDPIRAITMATFNVADYWRLERIGAIAPGYWANLVVLDDLRD
                     FNVREVYWQGQRVAAGGRPGFDVQTAIPAALRNSVHLPNLRAEMLRIPPEAANQAVGT
                     IDGQIVTTLLHVEPAIVDGQPVADVERDLLKLVCVERHRATGNVGAGYVKGFGLRRGA
                     LATSIAHDAHNVVAVGTNDADILLAIETLAAMQGGLAAVADGEVLAQLALPVAGILSD
                     QPLEATAAGYERMEAVARELGSSLHSPYGLLAFMALSVIPEARVTDQGFIVV"
     gene            complement(2634..3083)
                     /locus_tag="VFV93_10790"
     CDS             complement(2634..3083)
                     /locus_tag="VFV93_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165871.1"
                     /translation="MAVAEHRIALGPVVDIFKEAGFYAYRTLDEENNWCVACDLEEGH
                     IDIRIGDDGYELDVWATSPGLFIEEEDTRRRHALERLARVSLPGIRRGILDENQTIEW
                     DEQERGLSLRSRFILPFSINERMPAVALQQLAELNNALTAIERRIAS"
     gene            complement(3148..>3340)
                     /locus_tag="VFV93_10795"
     CDS             complement(3148..>3340)
                     /locus_tag="VFV93_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165872.1"
                     /translation="LSIDEIRARLHGAGGADATASESWRRLVLHPDLELHIRNGAPEA
                     VRTLAAELTEQARRWFGDE"
BASE COUNT          584 a         1155 c         1013 g          588 t
ORIGIN      
        1 tcgctgccgg gtgccctctg ggcggtggac gacagggaaa agcctactcg acttccagaa
       61 tcatctcgat ctcgacgggg atctgccctg gcagcgccgc catcccgacg gccgagcggg
      121 cgtgccggcc gttttcgccg aacagttcga ccatcaagtc cgagaagcca ttgatgacct
      181 tcggttgatc gccgaacccc ggcgccgagt tcaccatccc aagtaccttg acaaccttct
      241 tgattcggtc gaggtcgcca agcgcttcct tcgccgacgc cagcaggttc agtgtcgtcg
      301 acctggccgc ttcgtacgcc tcttccagag agacatccgc tccgaccttg ccggcgtaga
      361 ccggcccacc gctgcgattc ggtccgtgac cggccatgaa gagcaggttc ccaacctgca
      421 cgtaacgcag atagttcgcg atcggcgccg gtgcgtccgg cagttccaac cccagttcct
      481 tcagccgctg ctcgacctgc attcgctccc tctccatccg ttctgcctgg cgatcgatgc
      541 gccatcttcg ccgaggattc ccagggacgc aatgaagatg gcgggcccta tcgtggtgcg
      601 gtcagcagca ggaagccgac gatcgtactg agcagaaaga ctcccggcag gaagatcagg
      661 tagaccgagc gcgctcgggt cgccacccgc attgtcacga tccgatagcc gaggaacgtc
      721 accagcagcg tgccgagcag caaagcgaca gcgacgatca catagaaggc gccgcgcgcg
      781 gcctcgatcg cgtccgggaa cggccacgag cgcagcatcg cgacatcgaa cagcgccagc
      841 aggatcgtca gaaccacggc ccagcgcccg atctgacgga tcagcagctt gtctatctcc
      901 gaccgtgacg cctcggtcgt catacgacga tgaatccctg atcggtgacg cgtgcctcgg
      961 ggatgaccga gagcgccatg aaggccagca acccgtatgg tgagtgcagc gacgaaccga
     1021 gctctctcgc gacagcttcc atccgctcgt agccagcggc tgtcgcctcc agcggctgat
     1081 cgctgagaat gccggccacc ggcagcgcaa gctgcgccag gacctcgcca tccgcgacgg
     1141 ctgccagtcc gccctgcatc gcggcgagcg tctcaatcgc cagcaggata tcggcgtcgt
     1201 tggtgccgac ggcgacgacg ttatgcgcgt cgtgggcgat cgaggttgcc agcgccccgc
     1261 gccgcaagcc gaagcctttg acgtagccgg caccgacgtt tccggtcgcg cggtggcgct
     1321 cgacgcagac gagcttcagc agatcgcgtt cgacatctgc gaccggctga ccatcgacga
     1381 tcgccggctc tacgtgcagc agtgtcgtca cgatctgccc gtcgatcgtt ccgaccgcct
     1441 ggttggcagc ctccggcgga atccgcagca tctcggccct caggttgggc agatggacgc
     1501 tgttcctcaa tgccgccggg atcgcggtct gaacgtcaaa gcctggccgt cctccggcgg
     1561 caacccgctg cccctgccag tagacctcgc ggacgttgaa gtcgcgcagg tcgtcgagga
     1621 caaccagatt cgcccagtag ccgggcgcga ttgcgccgat ccgctccagc cgccagtaat
     1681 cagcgacgtt gaatgtcgcc atcgtgatgg ctcggatcgg gtccagcccc tcggaaaccg
     1741 ccagccgcag cgtctggttc atgtggccgt cgtgcagcag cgtcgtgcag tcgcggtcgt
     1801 cgctggcaaa gcagcagcgc gcccaggtcc ggtcattcac tagcggcaac agctcgcgaa
     1861 ggttgtgctc ggtcgatccc tcgcggatca tcagcatcat cccgcgtcgc agcttttcgc
     1921 ggccctcctc cagactcgtc gattcatggt cgcttgtcat cccggagagc gcatacccgt
     1981 caagtgccct tccagtgagc ccgggcgcat ggccgtcctt tcgccagccg ttggccgctt
     2041 ccagcctggc gaagatctca gggtcgccgc cgagcacgcc ggggaagttc atcatctccc
     2101 ccagaccaac cgcggcgggg ttctgcagcc cggccgcgat gtcccccgcc gagatgaccg
     2161 ctccggccgt ctcacgcggg ctggccggca cgcagcttgg caccgtgaaa tagaccgcca
     2221 gcggcagatc gcggctggcg tcaacgagtg cgttgaaccc ggaaagaccg gcgacgttga
     2281 cgatctcgtg cgggtcggtg acgatcgcgc cagtaccatg ggggacgacg gtccgcgcga
     2341 attgggtgat ccacagcagc gagctctcgg tgtgaaagtg cccgtcgatg aatgacggcg
     2401 cgacgaaggc cccatccagg tcgacaaccc gctcggcagc ggtgtagtca ccgatcccga
     2461 cgatgcgacc gtccgcaatc gcgatggtgc catgctcgac ctcgccggtg aagacgttga
     2521 cgacctgccc gccgcgcagc agcagatcgg ccgcctcacc gccagtcgcg atcctcaacg
     2581 cccgctgcca gtgttcaagc acggccctct cctctttcag acgaattggc gacttagctt
     2641 gcgatcctgc gctcgatcgc cgtcaacgcg ttgttcagtt cagcgagctg ctggagcgcg
     2701 acagccggca ttcgctcgtt gattgagaac ggcaagatga agcgcgagcg cagcgacagg
     2761 ccccgctcct gctcgtccca ctcaatcgtc tggttctcgt cgagaatccc gcgccggatc
     2821 cccggcagcg acacgcgggc cagccgttcg agcgcatggc ggcggcgcgt atcttcctcc
     2881 tcgatgaata gccccggcga cgttgcccag acgtccagtt cgtatccatc gtcaccgatt
     2941 cgaatgtcga tgtgtccctc ttcgagatcg caggcgacgc accagttgtt ctcctcgtcg
     3001 agcgtgcgat aggcgtagaa gccggcctcc ttgaagatgt cgacgaccgg cccgagtgcg
     3061 atgcgatgtt ctgcgacagc cacggttgct acgtctttcc agttggcagt taccacgatg
     3121 ccatccagcc tcaattgtac gaagttgtca ctcgtcgccg aaccagcggc gagcctgctc
     3181 cgtcaattcg gccgccagcg tccggaccgc ctcaggcgct ccattgcgaa tgtgcagttc
     3241 caggtcggga tgcagcacca gccgtcgcca gctctcgctg gcggtggcgt cggcgccccc
     3301 cgcgccatgc aatctggcgc gaatctcgtc gatgctcaac
//
LOCUS       DASUGJ010000482         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1881145, whole genome shotgun sequence.
ACCESSION   DASUGJ010000482 DASUGJ010000000
VERSION     DASUGJ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881145"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /gene="treS"
                     /locus_tag="VFV93_10800"
     CDS             complement(<1..1306)
                     /gene="treS"
                     /locus_tag="VFV93_10800"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX5165873.1"
                     /translation="MHIKAFSDSTNDGIGDFTGLLQKLDYVQSLGVDTIWVLPFYPSP
                     LRDDGYDIADYRDVHPAYGTLRDVRRFIKAAHQRGLRVITELVINHTSDQHHWFQRAR
                     RAKRGSSHRNYYVWSDTDQRYPETRIIFLDTEPSNWTWDPVAQQYFWHRFYHHQPDLN
                     FDNPAVMREILRVLHFWLDMGIDGLRLDAIPYLIERDGTINENLPETHAILKRIRAEI
                     DRHYPGRMLLAEANQWPEDVLPYFGDGDECHMAFHFPLMPRMYMAIAQEDRHPIADIL
                     RQTPELPPCCQWGIFLRNHDELTLEMVTNRERDYLWNVYASDRRMRLNLGIRRRLAPL
                     LENDRRKIQLMNSLLLSMPGTPIIYYGDEIGMGDNIYLGDRNSVRTPMQWTPDRNGGF
                     SRSDPAQLFLPCIMDPVYGYHAVNVEAQTRSLSSLLNWMKRLI"
     gene            complement(1390..>2198)
                     /locus_tag="VFV93_10805"
     CDS             complement(1390..>2198)
                     /locus_tag="VFV93_10805"
                     /inference="COORDINATES: protein motif:HMM:NF044853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX5165874.1"
                     /translation="PVPSWEWMIREVLDRHPDVIFLSEAFTRPKMMKHLAKVGFAQSY
                     SYFTWRNTKAELTEYLTELTQDEPREYMRPNFFANTPDINPVPLQTSGRAGFQSRLVL
                     AATLSTLYGIYNGFELCEGTPIPGKEEYLDSEKYEIKAWDDDRPGNIRDYIARVNRIR
                     RENPALREFTNLRFYNAWNDQVLVYGKMTAARDNIVLIAVNLDPHHAQSASFEVPLWE
                     LDLPDWANVEVEDLFDGHSFVWHGKVQQVWLDPHVNPCAIWRITPPGLAR"
BASE COUNT          377 a          654 c          741 g          426 t
ORIGIN      
        1 tgatcaggcg cttcatccag ttcagaagcg acgagagtga acgagtctgt gcctccacgt
       61 tgacggcgtg atatccgtac accggatcca tgatgcatgg caggaaaagc tgggccggat
      121 ccgagcgcga gaacccgcca ttgcggtcgg gcgtccattg catcggcgta cgcacgctgt
      181 tccggtcgcc caaatagata ttatctccca tgccgatctc gtcgccgtag tagatgatcg
      241 gcgtgccggg catcgacagc aacaggctgt tcatcagttg aatcttgcgc cgatcgtttt
      301 cgagcaacgg cgccaggcgg cgacggatcc cgagattgag ccgcatgcgg cgatccgacg
      361 cgtagacgtt ccacagatag tcgcgctcgc gattggtgac catctcgagc gtgagctcgt
      421 catggttgcg caggaagatg ccccattggc agcacggcgg cagctccggc gtctggcgca
      481 gaatgtcggc gatcgggtgc cggtcctcct gggcgatcgc catgtacatg cgcggcatca
      541 gcgggaagtg gaaagccatg tggcactcgt cgccatcgcc gaaatagggc agcacgtcct
      601 cgggccactg gttggcttcg gcgagcagca tgcgccccgg gtagtgccgg tcgatctcgg
      661 cccggatgcg cttgaggatc gcatgcgtct ccggcaggtt ctcgttgatc gtgccgtcgc
      721 gctcgatcag gtaggggatc gcgtccaggc gcaggccgtc gatgcccatg tcgagccaga
      781 agtgcagcac gcgcaggatc tcgcgcatga ccgccgggtt gtcgaagttg aggtccggct
      841 ggtggtggta gaagcggtgc cagaagtact gctgcgcgac cggatcccac gtccagttcg
      901 atggctccgt gtcgagaaag atgatccgcg tctctggata gcgctgatcc gtgtcgctcc
      961 acacgtaata attgcgatgc gacgatccgc gcttggcgcg ccgggcgcgc tggaaccagt
     1021 gatgctggtc ggacgtgtgg ttgatcacga gttcggtgat cacccgcaga ccgcgctggt
     1081 gcgccgcctt gatgaagcgg cggacgtcgc gcagcgtgcc gtaagccgga tggacgtcgc
     1141 ggtaatcggc gatgtcgtac ccgtcgtcgc gcaacggcga cgggtagaag ggcagcaccc
     1201 agatcgtgtc gaccccgagc gactggacgt agtcgagctt ctgcagcagg cccgtgaagt
     1261 cgccgatgcc gtcgttcgtt gaatcggaga acgccttgat gtgcaactgg tagatgatgg
     1321 catccttgta ccagtccgcc tgatcttgat cgatcatgcc ccgatggtct ctcggtcggt
     1381 tcggcgtgct cagcgcgcca ggcccggcgg cgtgatccgc cagatcgcgc agggattcac
     1441 atgcgggtcg agccagacct gctgcacctt accgtgccag acgaagctgt ggccgtcgaa
     1501 gaggtcctcg acctcgacgt tcgcccagtc cggcaggtcg agctcccaga gcggcacctc
     1561 gaacgacgcc gactgggcgt ggtgcggatc gagattgacg gcgatcagca cgatgttgtc
     1621 cctggcggcg gtcatcttgc catagacgag gacctgatcg ttccaggcgt tgtagaagcg
     1681 caggttcgtg aactcgcgca gtgccgggtt ctcgcggcgg atgcggttca cccgggcgat
     1741 gtagtcgcgg atgttgccgg gccggtcgtc gtcccaggcc ttgatctcgt acttctcgga
     1801 atcgagatat tcctccttgc ccgggatcgg cgtaccctcg cacagctcga agccgttgta
     1861 gatgccgtac agcgtcgaca gggtcgccgc cagcaccagg cgcgactgga agccggcccg
     1921 cccgctggtc tgcaggggca ccgggttgat gtcgggcgtg ttggcgaaga agttcggccg
     1981 catgtactcc ctgggctcgt cctgggtcaa ctcggtcagg tactcggtca gctcggcctt
     2041 cgtgttgcgc caggtgaagt atgagtagga ctgcgcgaag ccgaccttgg cgaggtgctt
     2101 catcatcttc gggcgcgtga aggcctcgga gaggaagatc acgtcgggat gccggtcgag
     2161 cacctcgcgg atcatccatt cccaggacgg caccggct
//
LOCUS       DASUGJ010000483         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1881418, whole genome shotgun sequence.
ACCESSION   DASUGJ010000483 DASUGJ010000000
VERSION     DASUGJ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881418"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /locus_tag="VFV93_10810"
     CDS             complement(<1..1743)
                     /locus_tag="VFV93_10810"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEX5165875.1"
                     /translation="MPEYLAPGVYVEEVSYRAKSIEGASTSIASCLGPTRYGPVGGEP
                     ELLTSFADFERIFGGIDNVGFEAAGGGIDFDQINYMAHSVRAFFDNGGTKLYVTRVHQ
                     AREGANAAETEALNGGRASTASADPAVTLQARFPGQAGNMRVVISVLATPNLLVPDTN
                     ASDPQVRGQVRGTREHDVLLVRDASSPLGPIEEGLYDVILLPDRTLGIRVGSPLEPTK
                     LTYLDPAEVSVHRVVLNVRVTRLGRFEDEQVWTELPPHPDARNSMMEYFTMQPSSRLR
                     YLTVPFAIVSEDDEILGSGARLVDALLGDGVINALNAAFDGSGPRPGDPDLEHEYHLT
                     GGADGVLPNAESYRGQEATSADDVSTGLVTFEDIDEISMVAAPGHNAEGTDLLRSAQI
                     VQSLITHCQILMRYRVAILDAPQNQIVSEVREYRSQFDSTHAALYYPWITTIDPLDPE
                     GRREIELPPSGFVAGIYARTDVLHGVFKAPANEVVLGAIGFETLLNKRQQDVLNPEGI
                     NCFRFFEGRGYRLWGARTISSDPEWKYISVRRYFAYLEHSIDKGTQWAVFENNGDRLW
                     ANIRLTVEDFLFNEW"
     gene            complement(1771..2661)
                     /locus_tag="VFV93_10815"
     CDS             complement(1771..2661)
                     /locus_tag="VFV93_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165876.1"
                     /translation="MSSPVVTTPTFQLFEPLEQITIFTPLGIRFWDAALDRQVTDGLV
                     VTARRPGSNQRPVRAFRTRSGIYAFRGLPGLRAIEYPVDDADRLGGSPPALTGFFVEV
                     VDEERRFVPTVFTVDVPFEGIYPTGSSDPMAPPGVYLFSAPTRPVASSLAVVRAQLAE
                     RPAGGEEQPAAFALLEVEVAGRPPAFGIADELGNVAVQFPYPRFSTALPNGSPPASAD
                     GSASQRWPIRVRVRFDPPALDVPIGSSIPTLRSIFSQRPGAIQAGSGPAPEFATELAA
                     GRQVVLRHSPGSTFVISRAI"
     gene            complement(2669..>3212)
                     /locus_tag="VFV93_10820"
     CDS             complement(2669..>3212)
                     /locus_tag="VFV93_10820"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEX5165877.1"
                     /translation="QYQPEDFEDNQLEFKVYVAKDFATPMDAGVSAFLYRIYPNGSHR
                     TPSGRLDPFGRRYRTQLPLDLHFLLTAWAKDASLQHTIAGWMMRVLEDTPSFPPGLLN
                     STFDNVFHPDETVEIGLTELSTEELFRIWEVIGEKAYQLSIPYVARTVRIESRLPSGD
                     GEPVQERVFRAGLVSMPGGG"
BASE COUNT          565 a         1070 c         1069 g          508 t
ORIGIN      
        1 ccactcgttg aagaggaagt cctcgaccgt cagccggatg ttggcccaga gccggtcgcc
       61 gttgttctcg aagacggccc actgcgtccc cttgtcgatc gagtgctcca ggtaggcgaa
      121 gtagcgccgg acgctgatgt atttccactc cgggtcggaa ctgatcgtcc gcgcgcccca
      181 gagccggtag ccgcgcccct cgaagaagcg gaagcagttg atcccctcgg ggttgaggac
      241 gtcctgctgg cgcttgttga gcagcgtttc gaagccgatc gccccgagga cgacctcgtt
      301 ggccggcgcc ttgaagacgc cgtgcaggac atcggtccgc gcgtagatgc cggccacgaa
      361 gccgctcggc ggcagctcaa tctcgcgccg cccctccgga tcgagcgggt cgatcgtcgt
      421 gatccacggg tagtagaggg cagcgtgggt cgagtcgaac tgactgcgat actcgcgcac
      481 ctcggagacg atctggttct gcggcgcgtc cagaatcgca acccggtagc gcatcaggat
      541 ctggcagtgg gtgatcagcg actgcacgat ctgggccgag cgcagcaggt cggtcccttc
      601 cgcgttatgt cccggcgcgg cgaccatcga gatctcgtca atgtcctcga acgtcaccag
      661 cccggtcgag acatcgtcgg cgctggtcgc ctcctgaccg cgatacgact ccgcgttggg
      721 aagaactccg tcggcgccgc cggtcaggtg gtactcgtgt tccagatcgg gatcgcccgg
      781 ccgtggcccg ctgccgtcga aggcggcgtt cagcgcgttg atgacgccgt cacccaacag
      841 cgcatcgacc agccgcgcac cggagccaag gatctcgtcg tcctccgaga caatcgcgaa
      901 cgggacggtc aggtagcgca gccgcgagct gggctgcatc gtgaagtact ccatcatcga
      961 gttgcgcgca tcggggtgcg gcggtagctc ggtccagact tgctcgtcct cgaagcgccc
     1021 cagccgggtg acgcgcacat tcaggacaac acgatggacc gagacctcgg ccggatcgag
     1081 ataagtcagc ttcgtcggct ccagcggcga gccaacccgg atccccaacg tgcgatcggg
     1141 aagcaggatg acatcgtaga gcccttcctc gatcggtccg agcggcgagc tggcatcgcg
     1201 aaccagcagg acatcgtgct cccgcgtgcc gcgtacctgc ccgcgcacct gcgggtcact
     1261 ggcattcgta tccggcacca gcaggttcgg cgtggcaagg acggagatga cgacgcgcat
     1321 gttgccggcc tggccgggga agcgcgcctg cagtgtcacc gccggatcgg ccgaggcagt
     1381 tgacgcgcga ccgccattca gcgcttccgt ctcagccgcg ttcgcgccct cgcgagcctg
     1441 atgcacgcga gtgacataca gcttcgtgcc gccgttgtcg aagaacgcgc ggacggaatg
     1501 tgccatgtag ttgatctggt cgaagtcgat gccgccgccg gctgcctcga aaccgacgtt
     1561 atcgatgccg ccgaagatgc gctcgaaatc ggcgaagctg gtcagcagct ccggctcgcc
     1621 gccgaccggc ccgtagcgcg tcgggccgag acagctggcg atgctggtgc tggccccttc
     1681 aatcgacttg gcccggtagc tgacctcttc gacgtagaca ccgggggcga gatactctgg
     1741 catccgatac ctccctgacg cgaaccggac ctagatggca cggctgatga caaacgtcga
     1801 accgggcgag tgacgcagga caacctggcg gccggcagcc aactcggtcg cgaactcggg
     1861 cgctggcccc gatccggcct ggatcgctcc gggccgctgg ctgaagatcg agcgcaacgt
     1921 ggggatcgac gacccgatcg ggacatcgag cgctggcgga tcgaaacgga cgcgaacacg
     1981 aatcggccag cgctggctcg ccgaaccgtc cgcgctcgcc ggcggggagc cgttcggcaa
     2041 tgccgtcgag aagcgtgggt acgggaactg gacggcgacg ttgccgagtt cgtcggcgat
     2101 gccaaacgcc ggtggccggc ccgcgacctc gacctccagc agcgcgaacg ccgccggctg
     2161 ctcctccccg ccggccggcc gctcggccag ttgcgcacgg acgaccgcca ggctcgacgc
     2221 aacaggtcgg gtcggcgctg agaacaggta gacacccggc ggcgccatcg gatcgctact
     2281 gccggtcggg tagatgccct caaatggcac atcgacggtg aagacggttg ggacaaagcg
     2341 acgctcttcg tcgacaacct cgacgaagaa gccggtcagc gcgggcgggc tgccgccaag
     2401 ccggtcagcg tcgtcgaccg gatactcgat cgcgcgcaac cccggcagcc cgcgaaaggc
     2461 gtagatgccc gagcgcgtcc ggaaggcgcg gacgggtcgc tggttcgagc cgggtcgccg
     2521 ggccgtcacg accagcccgt cggtcacctg ccggtcgagc gcggcgtccc agaagcggat
     2581 gccgagcggc gtgaagatcg tgatctgctc cagcggctcg aacagctgaa aggttggcgt
     2641 cgtcacgaca ggactgctca tctcaccctc acccgccacc cggcatcgag accagcccgg
     2701 cccggaacac ccgctcctgg accggctcgc catcgccgga cggtagcctg gattcgatcc
     2761 tgaccgtccg cgccacatac ggaatcgaga gctgataggc cttctcgccg atcacctccc
     2821 agatccggaa caactcctcg gtcgatagct ccgtcaggcc aatctcgacc gtctcgtcgg
     2881 ggtggaagac gttatcgaag gtgctgttga gcagcccagg cgggaagctc ggcgtgtctt
     2941 ccaggacgcg catcatccag ccggcgatcg tgtgctggag cgaggcgtcc ttggcccagg
     3001 ccgtcagcag gaaatgcaga tccagcggca gctgggtccg gtagcgacgg ccgaacgggt
     3061 ccagccgccc gcttggtgtc cgatggctgc cattcgggta gatgcgatag aggaacgccg
     3121 agacaccggc atccatcggc gttgcaaagt ctttggcgac gtacaccttg aactccagct
     3181 ggttgtcctc gaagtcctcc ggctggtact gg
//
LOCUS       DASUGJ010000484         5570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1744129, whole genome shotgun sequence.
ACCESSION   DASUGJ010000484 DASUGJ010000000
VERSION     DASUGJ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744129"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..1137
                     /locus_tag="VFV93_10825"
     CDS             298..1137
                     /locus_tag="VFV93_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5165878.1"
                     /translation="MHLLDIVHRTPVPEPWAEGEKIPWHEPGFSERMLAEHLSQAHDA
                     ASRRSGTIDAHADWIVRVVLGGRPSRILDLGCGPGLYIERLAGLGHACVGIDISPASI
                     RYAVATAESRGLTCTYHLGDIREASFGRGLDLIMLVFGEFNVFRPADARRILMKAREA
                     LAGDGQLLLEVHTSEAVRRIGSAQPSWRAAERGLFSDRPHLRLEEAFWDAGQQVATER
                     YYIVDTETAAVTRYAQSIQAYTDDEYGAILRDCGFALRGIHPSLGDVPGDGDFFVLLA
                     VAE"
     gene            complement(1166..2461)
                     /locus_tag="VFV93_10830"
     CDS             complement(1166..2461)
                     /locus_tag="VFV93_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5165879.1"
                     /translation="MGMAASLRAKTGSADDAVAIVESGQRVFVHGTCATPQALIAALV
                     RRAETLRDVRVTHLHTNGPAPYAEPGMEASFRHESLFTGSNVREAVNAGRADYIPAFL
                     SEVPILFRDGHLPIDVAFLNVSPPDAHGYCSLGTSVDCALSAARHARAIVAQINHAMP
                     RTLGDSFIHIDQIDRAVEVDQPPAEVPAAPPSEVELRIGGFVASLIEDGSTLQMGIGS
                     IPNAVLTSLGSHRDLGVHSEMFSDGVVELVERGVITGARNPLHPGKLLSSFVMGSATL
                     YAFVDDNPQVALHPVDYVNDTSVIRRNHRMVAINSALEIDLTGQVCAASIGDRVYSGI
                     GGQVDFMRGAALAPGGKPILALPSTAAGGTQSRIVGHLRPGAMLTLVQGNVHYVVTEY
                     GIADLYGKSLRQRAEALIAIAHPSFRDDLAAFARDLHRL"
     gene            complement(2552..3481)
                     /locus_tag="VFV93_10835"
     CDS             complement(2552..3481)
                     /locus_tag="VFV93_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5165880.1"
                     /translation="MKTIVVPLDGSELSEQAIGLGLSLARAWNAPLSLVHVLEEPVLL
                     DLLPGLALPNREDAEAYLRDVAATLPLDIAVSTHVLRGNPAATILDLTEDEPETVVVM
                     STHGRGGMRRLFGSVAGKVVHEAMTPVALVRSPATLHNQRLESILVPLDASNFSETAL
                     PVAADLARRTGATLGLTHVCEPFWVSPGVASIPELAQINAARMSDVERESLNDARAYL
                     DRVAGYLRSEGIRVVWEVRFGRPADEIMRAAETTGADLVVMATHDRRGLQRLALGSVT
                     NEVLQHGPTPVLTIPPRLIEQRQRDAADMLSTF"
     gene            3687..4397
                     /locus_tag="VFV93_10840"
     CDS             3687..4397
                     /locus_tag="VFV93_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5165881.1"
                     /translation="MQKRLILVADDDAPILRLVRTKLQTDGYHVVTATNGQEALDRFE
                     VDRPDLIVLDIMMPVMDGIEALRRIRTQSNVPVILLTARTAGRDKVRSLDLGADDYVT
                     KPFDPDELSARVAAVLRRATIAAAPAEQVLHYDGVTIELTNRRVLVGDEEIHLSRTEW
                     EVLSLLAANPGRVMTHGELLGRIWGPAFRDETSYLRTWISRIRKKLGDTQESSPLIRT
                     FPGIGYRLESPPDNGHAG"
     gene            4387..>5570
                     /locus_tag="VFV93_10845"
     CDS             4387..>5570
                     /locus_tag="VFV93_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5165882.1"
                     /translation="MQGDVAGPVGEALSIALGLPVRELRELEPDVQQEALIALPGRIG
                     EPVVFSTGSCDYLACRYHPQRHAVLILGPYRLVAGPVTASDELDAAGLERAGRALQQA
                     VEGLRRATDASVQRIELASQIEVSSRAILAISSALSLDTVLNRIVDLAREISGARYAA
                     LGVPGPSGELESFLTSGISSEEASAIAHPPAGRGLLGLLLREPGTVRLANLAEHPASV
                     GFPANHPPMRSFLGVSIMAHGKVLGNLYLTEKRTGRAFTDDDARLVEELARHAAVAIE
                     NAHLYQQVGQQQQRLQLILDQLPEATLLAEREPERVTLANQHASNLLGWQIETPIPLE
                     TFLRRNPRLGPDDSPMPAESLPMMQSLRSGERVSHHEVRIKRADGQVITALVNSAPLR
                     GPG"
BASE COUNT          905 a         1753 c         1965 g          947 t
ORIGIN      
        1 gtccttcggc tgcggcctca ggacgacagg aagagacgtg tcacaggtct gtcacgctgg
       61 cgttccgaat gcgacctgcg tgctacggtt agcagcgtat cctccgcgtt gcggatcggc
      121 ggatcaagcg gaatcaggag gcggcaaatg gtacgacttc ggcttcctgg tcggcgaagg
      181 gtcgccgctg ctgggaacgc tcggtggcaa gcaccggtcg agtgcgccac gaagcgcgag
      241 ccgccggggt gatttcgctt gaatagcgcc ggagatcatc gttcgcgaga gtgagccatg
      301 cacctgctcg acatcgttca tcgaacgccg gtaccggagc cgtgggccga aggggagaag
      361 atcccctggc acgagccggg tttcagcgag cggatgctgg ctgagcacct ctcgcaggcg
      421 catgacgccg ccagccgccg gtccgggacg atcgacgcgc atgctgactg gattgttcga
      481 gttgtcctcg gtggccggcc gtcgcggatt ctcgatctgg gttgcgggcc ggggctatat
      541 atcgagcggc tggcggggct ggggcatgcc tgcgttggta tcgacatctc cccagcgtcg
      601 atcaggtacg ccgtcgcgac agcggaatcg cgcggtctga cctgcacgta tcacctcggc
      661 gatatccgcg aagcgtcctt cgggcgtggg ctggatctga tcatgctcgt gtttggcgag
      721 ttcaacgtct tccggccagc agacgcgcgg cggatcctga tgaaagcacg cgaggcgctg
      781 gccggcgatg gccaactgct gctggaggtc cacaccagcg aggccgtacg gcggatcggc
      841 tcggcccagc cctcgtggcg cgctgccgag cgcggcttgt tctcggatcg cccccacctg
      901 cgactcgaag aggccttctg ggacgccggg cagcaggtcg cgaccgaacg ctattacatc
      961 gtcgacaccg agactgcggc agtcacgcgc tatgcccaga gcatccaggc ctataccgac
     1021 gacgaatacg gcgcgatcct gcgtgactgc ggcttcgcgc tgcgtggaat ccatccatcg
     1081 ctgggcgatg ttcccggcga cggggacttc ttcgtgctgc tggcggttgc cgagtaggcg
     1141 tgcgcgtcgc cactgggtgc gatcgtcaca ggcgatgcag gtcccgcgcg aaggcggcaa
     1201 ggtcgtcgcg gaagctgggg tgggcgatgg cgatcagggc ttcggcgcgc tggcgcaggc
     1261 tcttgccgta caggtcggcg atgccgtatt ccgtcacgac ataatgtacg ttgccctgga
     1321 ccagcgtcag catcgcgccg gggcgcaggt ggccgacgat ccgcgattgt gtgccgccgg
     1381 cggccgttga cggcagtgcc aggatcggct tgccgcctgg cgcgagggcc gcgccgcgca
     1441 tgaagtcgac ctggcctccg atgccgctgt agacacggtc gccgatcgag gcggcgcaga
     1501 cctggccggt caggtcgatc tccagcgccg agttgatggc caccatccgg tgattgcggc
     1561 ggatgacgga cgtgtcgttg acgtaatcga cggggtgcag cgccacctgg gggttgtcgt
     1621 cgacgaaagc gtagagcgtt gccgagccca tcacgaacga actgagcagc ttgccggggt
     1681 ggagcgggtt gcgcgcgccg gtgatgacgc cccgttcgac cagctcgacg acgccgtctg
     1741 agaacatctc gctgtggaca cccagatcgc gatggctgcc gagcgaggtg aggaccgcgt
     1801 tggggatgct gccaatgccc atctggagcg ttgatccatc ctcaatcagc gaggcgacga
     1861 agccgccgat ccgcagctcg acctcggagg gcggtgcggc cgggacctcg gccggtggct
     1921 ggtcgacctc gacagcgcgg tcgatctggt cgatgtggat gaagctgtcg ccgagcgtcc
     1981 tcggcatggc gtggttgatc tgggcgacga tcgcgcgtgc gtggcgagcc gcgctgaggg
     2041 cgcaatcgac ggaggtgccc agcgagcagt agccgtgggc gtcgggcggt gagacgttga
     2101 ggaaggcgac atcgatcggc aggtgaccgt cgcgaaagag gatcggcact tccgagagga
     2161 aggcggggat gtagtcggcc cggccagcgt tgacggcctc gcggacgttc gagccggtga
     2221 agagtgactc gtggcggaag ctcgcttcca tgccgggctc ggcatacggc gccggtccgt
     2281 tggtgtggag atgggtgacg cgaacatctc gcagcgtctc cgcgcgccgg accagcgcgg
     2341 cgatgagtgc ctgcggtgtc gcgcaggtgc cgtggacgaa cacacgctgg ccggattcga
     2401 cgatcgcgac cgcgtcgtcg gcgctgccgg ttttcgcgcg caggctggcc gccattccca
     2461 tccgtcgcac cctccgtttc catgccaatg cggcaggtcc gacgacctgc cgcacgaact
     2521 gttcctgccg tcgggccgcc gaggatcggc ctcagaatgt tgacagcatg tcggccgcgt
     2581 cacgctgccg ctgctcgatc aggcgcggcg gaatcgtgag gaccggtgtc gggccgtgct
     2641 ggagcacctc gttggtgacg ctgccgagcg ccagccgctg gagtccccgc cggtcgtggg
     2701 ttgccatcac gaccaggtcg gcgccggttg tctcggccgc ccgcatgatt tcgtcggccg
     2761 gccggccgaa gcgtacctcc cagacgacgc gaatgccctc cgaacggaga tagcctgcca
     2821 cccggtcgag gtaggcgcgg gcatcgttga gcgactcgcg ctcgacatcg ctcatccggg
     2881 cagcgttgat ctgtgccagt tccggaatcg aggcgacgcc gggcgagacc cagaacggct
     2941 cgcagacgtg cgtgagcccg agcgtcgcac cggttcgccg ggccagatcg gcggcaaccg
     3001 gcagggccgt ctcgctgaag ttcgacgcat caagcgggac gagaatggat tcgagccgct
     3061 ggttgtgcag cgtcgccggg gagcgaacca gcgccaccgg cgtcatcgct tcgtgaacga
     3121 ccttgccggc gacgctaccg aagagccgtc gcatgccgcc ccggccgtgg gtggacatga
     3181 ccaccaccgt ttccggctcg tcttctgtca ggtccaggat cgtcgcggcc gggttgccgc
     3241 gcaggacatg tgtcgagacg gcgatgtcga gcggaagggt agctgccacg tcgcgcaggt
     3301 aggcttcagc gtcctcgcga ttgggcaggg ccaggccggg gagcaggtcg agcaggaccg
     3361 gctcttcgag gacgtggacg agtgacaacg gcgcgttcca ggcccgcgcg agtgacagcc
     3421 ccaggccaat cgcctgttcc gacagttccg atccatccag gggaacgaca atggttttca
     3481 tctctggctc cgtatcagca ggttgcgcgg cggcggtcac gctctcggtc taaccgtact
     3541 ggctcaagcg tggcagatcg cccgttgtgg atacgtcgca gccgggcggt ggtcgttaca
     3601 attccgtgtc ggtataggcc gggatctctc aggtggcgat tcctgggtgt tacagtcagc
     3661 gcaggtgcgg gaagaaggga gcgacgatgc agaaacggtt gatcctggtc gctgatgacg
     3721 acgccccgat cctgcggctc gtccggacca agctccagac tgatggctac cacgtcgtga
     3781 cggcgacgaa cgggcaggag gcactcgacc ggttcgaggt cgaccggccc gacctgatcg
     3841 ttctcgacat catgatgccg gttatggacg ggatcgaggc gctgcggcgg attcgaaccc
     3901 agtcgaacgt gccggtcatc ctgctgacgg cccgaacggc cggccgcgac aaggtgcgca
     3961 gtctcgacct cggcgccgac gactacgtca ccaaaccatt cgatcccgac gagctctcgg
     4021 cgcgggtggc ggccgtcctg cggcgggcaa cgatcgctgc cgcgccggca gaacaggtgc
     4081 tgcactacga cggcgtgacg attgagctga ccaaccggcg cgtgctggtc ggcgacgagg
     4141 agatccatct cagccgaacg gagtgggagg tactgagcct gctggcggcc aatcccggcc
     4201 gggtcatgac gcacggcgaa ctgctggggc gtatctgggg ccccgccttc cgcgacgaga
     4261 catcctacct gcggacgtgg atcagccgca ttcgtaagaa actgggcgat acgcaggagt
     4321 cctcgccgct gatccggacc ttccccggca tcggctatcg gctcgaatcg ccccctgaca
     4381 acggccatgc agggtgacgt tgccggaccg gttggcgagg cactctcgat cgcgctcggg
     4441 ctgccggtgc gcgagttgcg cgagctggaa cctgatgtcc agcaggaggc gctgatcgcg
     4501 ctccccggcc ggatcggtga gccagttgta ttcagcaccg gttcatgcga ctatctcgcg
     4561 tgtcgctatc atccccaacg ccacgctgtt ctgatcctcg gcccgtaccg gctggtcgct
     4621 ggcccggtca ctgcgtcgga cgagctcgat gctgccggtc tggaacgcgc cggcagggcg
     4681 ctgcagcagg ccgtggaggg actccggcgg gcgacggacg ccagtgttca gcggatcgaa
     4741 ctggccagcc agatcgaggt ttccagccgc gcaattctgg cgatcagcag cgcgctgtcg
     4801 ctggataccg tcctgaaccg gattgtggac ctcgcccgcg agatttccgg tgcgcggtac
     4861 gcggcgctgg gtgtaccagg gccatccggt gaactggagt cattcctgac ttcgggcatc
     4921 tccagcgagg aggcatccgc gattgcccac cctccggctg ggcgcgggct gctcggactg
     4981 ctgctgcgtg aaccgggaac cgtccggctg gcgaacctgg ctgagcatcc tgcttcggtc
     5041 gggtttccgg ccaaccaccc gccgatgcgc agctttctcg gcgtgtcgat catggcgcat
     5101 ggcaaggtgc tcggaaatct ctacctgact gagaagcgga cgggccgcgc gtttaccgac
     5161 gatgacgcac ggctggttga ggaattggcc cggcatgcag ccgtcgcgat cgaaaacgct
     5221 catctctatc agcaggtcgg gcagcagcag cagcggttgc aactgatcct cgatcaacta
     5281 cccgaagcga cgctgctggc cgagcgtgag ccggagcggg tcacgctcgc caatcagcat
     5341 gcgtccaacc tgctcggctg gcagatcgag acaccgatcc cgctcgagac gttcctgcgg
     5401 cgcaacccgc gcctgggtcc ggacgattcc ccgatgccgg ccgaaagcct cccgatgatg
     5461 cagtcgctgc ggtctggcga gcgagtcagc catcatgagg tgcggatcaa acgagcagac
     5521 gggcaggtga taacggcgct cgtcaacagc gcgccgttgc gcgggccggg
//
LOCUS       DASUGJ010000485         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_75730, whole genome shotgun sequence.
ACCESSION   DASUGJ010000485 DASUGJ010000000
VERSION     DASUGJ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75730"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..589)
                     /locus_tag="VFV93_10850"
     CDS             complement(14..589)
                     /locus_tag="VFV93_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5165883.1"
                     /translation="MEDGDLVRRARAGDTDAYRCLMLRHQEIAFRAAFLITSDASEAE
                     DAAQDGFVNAFYALDRFRDGAPFRPWLLRIVTNQALNHRRTTGRHTSAIQRAGRAEPA
                     STHAPSPEAIILAAETRGLVLDALDRLDDRDRLVLSYRYLLDMPVAEIATILDCPQRT
                     VRSRITRALKRLATHLETDPDHSTRSERSRA"
     gene            833..1999
                     /locus_tag="VFV93_10855"
     CDS             833..1999
                     /locus_tag="VFV93_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5165884.1"
                     /translation="MTKKILIVGGGFAGFWAAMAARRVAGQQVAVTMLSREPVLQLRP
                     RLYEANPEALGVDLMPLLATVGVDFVAGEAIGLNPVGKHVELATGERLGYDRLVIATG
                     SVMRRPPVAGADVAFSIDTQADAIAFDRRLREIARTTSQPSIAVVGAGFTGIELALEM
                     RTRLAIHGGDEVAERAAVTLIDREPVVGFELGEGPRSEIEAALAAARIELLLGATVTE
                     LAADRLVFADGTVRAADAVVLTTGMVAAPFVSQVPGDRDRLGRLVVDSWLRAPAAPDI
                     FVTGDAAAADTGEGYLTLQSCQHALTLGPFAGENVARDLLGLPLLPYAQPRYVTCLDL
                     GRFGAVYTDGWDRIVRETGDVAKARKRRINTVVIYPPANATGEQLLELSTVSSD"
     gene            2072..2437
                     /locus_tag="VFV93_10860"
     CDS             2072..2437
                     /locus_tag="VFV93_10860"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX5165885.1"
                     /translation="MDQQIREQLQQGQTIDITTTGRKSGKPSRIEIAFQNIDGTVYIS
                     GLPGKRDWYANLVANPAFTFHLKQSLTADLPAQARPITDPAERRAVLTPFTTNWNYAK
                     DLEKWMDGSPLVAVSFDGE"
BASE COUNT          419 a          745 c          861 g          455 t
ORIGIN      
        1 gatgatgggc aggtcaggca cgggagcgct ccgatcgggt gctgtggtct gggtcggtct
       61 caaggtgggt cgccaggcgt ttgagcgcgc gggtgatccg cgagcggacg gtgcgctgcg
      121 ggcagtcgag gatcgtcgcg atctcggcga ccggcatatc gagcaggtag cggtaggaga
      181 ggaccagccg atccctgtca tcgagccggt cgagcgcgtc cagcaccagc ccccgcgtct
      241 cggcggcgag gatgatggct tccggcgatg gcgcatgggt ggaagccggc tcggcccgac
      301 cggcgcgctg aatagcgctg gtgtgccgac cagttgtccg gcggtggttg agcgcctggt
      361 tggtgacgat cctcagcagc cagggacgga atggcgcgcc gtcccggaag cgatcgagtg
      421 cgtagaaggc gttgacgaag ccgtcctggg cggcgtcctc ggcctcgctt gcgtcgctgg
      481 tgatgaggaa cgcggcccgg aaggcgatct cctggtgacg cagcatcagg cagcggtagg
      541 cgtcggtgtc gcctgctcgc gctcgcctga cgaggtcgcc atcctccacg cttggctccg
      601 atccggtctg ttgcgttcgc cggtcctgcc ctgtatacac cggtcgtctt caagatgact
      661 caggtatcaa cacctctgga gcgatccccg tgttgtcatc ctgcggtcgc agccgtcgga
      721 actgtccacg ggacacccca attggtactg gggtcgatat gccaactggt tctgttccga
      781 ggccgctcag gacggacaat catgctagcc tgagttggag aggggacgac cgatgaccaa
      841 gaagatactg attgtcggcg gtgggtttgc cggattctgg gcggcgatgg ctgccaggcg
      901 tgtcgcaggg cagcaggtag ccgtgacgat gctgtcgcgc gaaccggttc tacaacttcg
      961 accgcggttg tacgaggcga acccggaggc actcggcgtc gatctgatgc cgctgctggc
     1021 gacggtcggg gttgattttg tcgcgggaga ggcaattggg ctcaatccgg tgggcaagca
     1081 tgtcgagctt gcaactggcg agcggctagg ttacgaccgg ttggtgatcg cgactggcag
     1141 tgtgatgcga cggccgccgg tggcgggcgc cgatgtagca ttctctatcg atacccaagc
     1201 cgacgccatc gcgttcgatc ggcggctgcg cgagattgca cggacgacat cccagccgtc
     1261 gatcgccgtc gttggggcag gctttactgg catcgagctc gcgcttgaga tgcgtacacg
     1321 gttggctatc catggcgggg acgaggttgc tgagcgggca gccgtgacgc tgatcgaccg
     1381 ggaaccggtg gtcggtttcg agcttggcga agggccgcga tccgagatcg aggcggcact
     1441 ggctgccgcg cggattgagc tattgctggg cgcgaccgtc accgagctgg cagcggaccg
     1501 gctggtgttc gcggatggaa cggttcgcgc agccgacgcc gttgtcctga cgaccgggat
     1561 ggttgcggcg ccgttcgtca gccaggtgcc gggcgatcgg gatcggctgg gccggctggt
     1621 cgtcgattcg tggctgcggg cgccggccgc gccagacatc ttcgttaccg gcgatgccgc
     1681 tgctgccgat accggcgaag gttacctgac gctgcaatcc tgccagcacg cgctaacgct
     1741 tggcccgttt gccggtgaga acgtcgcccg cgatctgctc gggttgccgt tgctgccgta
     1801 cgcccagccg cgctatgtca cctgcctgga tttgggccga ttcggcgcag tctatacaga
     1861 cggctgggat cgtatcgtcc gcgagaccgg cgatgtggcc aaggcgcgta aacggcggat
     1921 caacactgtc gtcatttacc caccggccaa cgcgaccgga gagcagctgc tggagctgtc
     1981 aactgtttcg tcggactgac gatctggaat agcgtgtgcc gtccactggt taggagacga
     2041 caaccgccga accgtggaag gatcaccgtc attggaccag cagattcgag agcagcttca
     2101 acaggggcag acgatcgaca tcacgacgac cggccgcaag agcggcaagc cgagccggat
     2161 cgagatcgcg ttccagaaca tcgacggcac cgtctacatc tcaggcttgc ccggcaagcg
     2221 cgactggtac gcgaacctgg tcgcgaatcc cgccttcacc ttccatctca agcagtcgct
     2281 gacggccgac ctgccggccc aggcccgccc gatcaccgac cccgccgagc gccgcgcggt
     2341 cctgacgccg ttcactacca actggaacta cgccaaggat ctcgaaaaat ggatggatgg
     2401 cagcccgctg gtggccgtct cgttcgacgg cgaatagctc caataatcaa tcttccaatc
     2461 cggtacgggc gatatcgcaa
//
LOCUS       DASUGJ010000486         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_494114, whole genome shotgun sequence.
ACCESSION   DASUGJ010000486 DASUGJ010000000
VERSION     DASUGJ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494114"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..955)
                     /locus_tag="VFV93_10865"
     CDS             complement(263..955)
                     /locus_tag="VFV93_10865"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX5165886.1"
                     /translation="MVINDAMWIIFWSMFFSRFPVVRGWTLDDVVTLWAVAATGYGIA
                     WGFFGNVRPEGARVIVSGRMDYYLSLPRNVLLHFIGGTVSVASFGDIIFGLGVFAVVV
                     RPDAMQFVLFLMLSLCAAVILISFGLMVVSLAFWAGNTEGLGLQISNALITFSTYPLD
                     LFSTTVKVMLFTIIPAGFATYVPVTIIREFDPLRFAIVIAFTAAMAGLAAAVFYAGLR
                     RYESGSLVATQG"
     gene            complement(1062..1910)
                     /locus_tag="VFV93_10870"
     CDS             complement(1062..1910)
                     /locus_tag="VFV93_10870"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX5165887.1"
                     /translation="MTAPAVPISHRRAAGRLALITRKYTAIFRLSLMTQLAYPAEMWL
                     RTIFVFIIMFIFSSLWHTTYAEMGASTLGGLTLSQMLFYVAITESILLSRPRDAMRID
                     EEVRTGQLAYQLARPYNYLLYRFAQMWGERLPRFLTTLAVAATLAALFSGGVGVTPAG
                     LAMGLPALVLALAIDFLLVTTISLFAFWVEDTTPFLFIYDRFLMILGGMLLPLELFPD
                     VLETIARILPFSAVVYVPARMFVDPDGGRLAGLIVQQLVTLAVVAVVGLRLFRYAERR
                     LQSNGG"
     gene            complement(1907..>2616)
                     /locus_tag="VFV93_10875"
     CDS             complement(1907..>2616)
                     /locus_tag="VFV93_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5165888.1"
                     /translation="RTKLAFHIASVFGQKSQLSFHLPPRDSFDLLAAIYELDGTAYRA
                     RLAELVDLFEIGRLMDQPVRKLSLGERMRCEVAASLLHRPAILFLDEPTIGMDVIARQ
                     TIRDLIQRLNQEEGTTVLLTSHDAGDVEQICRRAIVINHGRIVFDDTISTLRRDYLGH
                     KVIDLTLAERAPPIDYANVRCIEHSDYRLRLEVDTSAQQLDLIVAHLVSRLRVADIQI
                     MDPPMEDVIAAIYRRAS"
BASE COUNT          568 a          800 c          824 g          424 t
ORIGIN      
        1 tacctgccgc cgctggagtg gcttcgtgaa cgtcgtgatt tgaaacctgt gcctcagtcc
       61 ggtcatcagc ctgaccgtca gtcgaacagg cgatcgacag catcgttccc gccaatgcga
      121 gcaggatccc acatgcagcg agccgtgcga tcaggttcat tcgtctcatg cagcggtcac
      181 cctcaggaca ttccatcaag cgatgcgtcg gagggtcaaa catcgcacga cataacgaag
      241 ccctaccggg gacgtgaagg acctacccct gggtggcgac gaggctgccg gactcgtatc
      301 gccgcagacc agcgtagaag acggcggcgg cgaggccggc catcgccgcc gtgaacgcga
      361 tgacgatcgc gaaccgtagt gggtcgaact cacggatgat cgtcaccggc acgtaggttg
      421 caaaaccggc cggaatgatc gtaaagagca tcaccttcac ggtcgtgctg aaaaggtcga
      481 ggggataggt ggagaacgtg atcaatgcgt tgctgatctg caaccccaat ccctccgtgt
      541 ttcccgccca aaacgccagg ctgacgacca tcaatccgaa gctgatcagg atcaccgctg
      601 cgcagagcga gagcataaga aacagcacga actgcatcgc gtccggacgt acgaccacgg
      661 caaacacgcc cagtccgaag atgatgtcgc cgaaggacgc cactgaaacg gtgccgccga
      721 tgaaatgcag caggacattg cgcggcagag acagatagta gtccattcga ccggagacga
      781 tcacgcgcgc gccctccggc cgcacgttgc cgaagaaccc ccaggcgatg ccgtaacccg
      841 tcgccgctac tgcccacaag gtgaccacat cgtcgagcgt ccaaccgcgc acgaccggga
      901 accgcgagaa gaacatcgac cagaagataa tccacatcgc gtcgttgatc accatgaagc
      961 cggcgctggt gaggaacccg acccgatagg ccatcgccga gcgcaggttg aaccagatga
     1021 gttgccccag gaaccggatc gtgctcggca ggcgcatttc gtcagcctcc gttcgattgc
     1081 agccgccgct cggcataccg aaacagccgc aacccaacga ccgccaccac agccagagtg
     1141 accagttgtt ggacgatcaa tcctgccaaa cgaccgccgt ccgggtcgac aaacattcgt
     1201 gctggtacat agacgaccgc gctgaacggc aggatccgag cgatcgtttc gagcacgtcg
     1261 ggaaatagct caagcggcag caacatgccg ccgaggatca tgagaaaccg atcgtagatg
     1321 aagagaaacg gcgtcgtgtc ctctacccaa aaggcaaaca ggctgatcgt ggtcaccagc
     1381 aggaagtcga tcgccagcgc cagcacgagc gcgggcagtc ccatcgccag gccggcgggc
     1441 gtgacaccaa cgccgcctga gaacagcgcg gcaagggttg cggcgacggc cagcgtcgtc
     1501 agaaaacggg gtaatcgctc gccccacatc tgcgcgaacc gatacagcag gtagttatac
     1561 gggcgggcca gttggtacgc cagctggccg gtgcggactt cttcgtcgat ccgcattgcg
     1621 tctcgcggtc gggagaggag gatgctctcg gtgatcgcca catagaagag catctggctc
     1681 agagtcaggc cgcccagcgt cgacgcgccc atctcggcgt aggtggtatg ccagaggctg
     1741 ctgaagatga acatgatgat gaagacgaag atcgtccgca gccacatctc ggccgggtag
     1801 gcgagctgtg tcatcaggct cagtcgaaag atggccgtgt atttccgagt gatgagggcg
     1861 aggcgtccgg ccgctcgccg gtggctgatc ggaacggcag gcgcggtcat gatgccctcc
     1921 gatagattgc ggcgattacg tcctccatcg gcgggtccat gatctggatg tcggcgaccc
     1981 gcagccggct gaccaggtgc gcgacgatca ggtccaattg ttgggccgag gtatcgacct
     2041 ccaaccgcag ccggtagtcg gagtgctcga tgcagcggac gttcgcgtag tcgatcggtg
     2101 gcgcccgctc ggccagcgtc aggtcgatca ccttgtggcc gaggtaatcc cgtcgcaacg
     2161 tgctgatcgt gtcgtcgaag acgatgcggc cgtggttgat gacgatcgcg cggcggcaga
     2221 tctgctcgac atcgcccgcg tcatgcgagg tgagcagcac cgtggttccc tcctcctgat
     2281 tgaggcgctg gatcaggtcg cggatcgtct gacgggcgat cacgtccatg ccgatcgtcg
     2341 gctcgtcaag gaagaggatc gccgggcgat gcaggagact ggcggcgacc tcgcagcgca
     2401 tccgctcacc caggctcagc ttgcgcaccg gctggtccat cagccggccg atctcgaaga
     2461 gatcgaccag ttctgccaag cgggcgcggt acgccgtccc gtccagttcg tagatcgccg
     2521 ccaggaggtc gaaactgtcg cgcggcggca ggtggaagga aagctgcgac ttctggccaa
     2581 acaccgaggc gatatggaag gcgagtttcg ttcgtt
//
LOCUS       DASUGJ010000487         4462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1326723, whole genome shotgun sequence.
ACCESSION   DASUGJ010000487 DASUGJ010000000
VERSION     DASUGJ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326723"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VFV93_10880"
     CDS             <1..1011
                     /locus_tag="VFV93_10880"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEX5165889.1"
                     /translation="IWWRVGITNPWPSEFAGMRLWRFSMRWDADWYVRIAEHGYTHVA
                     GQASTVGFFPGFPVAVAVAGAVLPGGTAFAGLVVAHLALIAALIYVFLLARLEFGERV
                     AWWTVGLLLMFPAGFFFSAVFPESLLLLGIAAALYHARRGQWWRAGLFGLLAGATGLA
                     GMLIVVPLALELWRQEGGRRIDVRDIVAVAIAPLGGLAYFGYLWARFGSPLVYFDSIA
                     AYRRDIRAFDSYRNGTGGSLDNVFAVSDAALIGVFLIAGALLWWRVRPSYGALVIGLT
                     LLPLVVGAPPGIGRQLVVAFPAFMLLGRIPSEGVRMMLSAVFSLGLMLTAFFFVQGFW
                     AG"
     gene            complement(1054..2271)
                     /locus_tag="VFV93_10885"
     CDS             complement(1054..2271)
                     /locus_tag="VFV93_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165890.1"
                     /translation="MPVKDTAREATIAAIRAARIEVTRACRDIETDSGKAERLNELLA
                     GHGFEVSSGVAGIADASRADRKHLDRAAMRNGLRHGHIAYLVDLDSPTSLGSATVLAA
                     AIGCGVALESEHGLVTVITYPGDAALLAIANAGVFDEFDAVFGARPAETGAGFAYTIN
                     STGDTLASRSADITVERDATAFLAQLEAETAQHDAPSRIDGSIEDGFVRLTLTARTSV
                     ELRDLSAIVQRLAAETPGASVSFGNPTDDMLVSRILARRVKTYADTLGYTFEQVAKLD
                     PEPATGWGNVSQVTPAFRLSFPFTTDQVVNGDPAFAAAAARSTSYDRALEFGECLGLA
                     GLDALRDMQFRAIADDQLIKALAARGVNRQHRRWLGVHPVIKDTNEKANGKKKGPRLA
                     DFRMVRGPGMRDN"
     gene            complement(2334..3131)
                     /locus_tag="VFV93_10890"
     CDS             complement(2334..3131)
                     /locus_tag="VFV93_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5165891.1"
                     /translation="MPTGIDHVIILVDDLDRGIEQYRKLGFTVTPGGKHPRYTHNALV
                     TFADGSYLELIAFYEQPTSTNPGEGHRWFKHVGKGGGIIDYALAAPNLDTLVADADTR
                     GYTHGAATPGARKRLDDVEIAWKSSMAKGDPNVGALPFLIEDVTDRGLRVPSESATHD
                     NTVRGIRSLVVAVQDLDAAVKRYCTLLDREKPSGEGLPNLDNADGVYFMVGEHRIDLA
                     TPTGDGPLADQLRARGDSPFELSLLARETRDIDPAQAGNARLRFVAG"
     gene            3339..4115
                     /locus_tag="VFV93_10895"
     CDS             3339..4115
                     /locus_tag="VFV93_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165892.1"
                     /translation="MPAGWPHHRTPRQIARSIALITLALALATACAGPETGDDRDPTP
                     TPTQTATPTEVPPTATTAASPTAASTDTPVPTTSASPTKAASPSVAASPTPTVVPLDE
                     RLPLLDDMPGAGYTIAEEGTRTAEELAEAYADPAAHLRRLDEWGFKRHIFRAFLLTDS
                     DDNRLPPNILATINLYGSDEQAEEALQWLRQLGTATGATDAEPPPIGDNAVALTVPTS
                     DGVPTASIYVRQGAILYVYFAEGGDPLPAVDSIATNVFSR"
     gene            4263..>4462
                     /locus_tag="VFV93_10900"
     CDS             4263..>4462
                     /locus_tag="VFV93_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165893.1"
                     /translation="MTDNAQSSEHGATELRNVRILRSKGLLAEYTTGQAPSGEWFALG
                     SVRSELPLTSPPAWILVGTGGSV"
BASE COUNT          698 a         1501 c         1453 g          810 t
ORIGIN      
        1 atctggtggc gggtggggat caccaatccg tggccgagcg aattcgcagg gatgcgtctc
       61 tggcgcttct cgatgcgctg ggacgccgac tggtacgtca ggatcgccga gcatggctac
      121 acccatgttg ccggtcaggc gtcgacggtt ggcttctttc cgggctttcc ggtcgcggtg
      181 gcggtggcgg gcgcggtgct gccgggcgga accgccttcg ccgggctggt cgtcgcccat
      241 ctggcgttga tcgccgcgtt gatctacgtc tttctgctcg cccggctcga atttggcgag
      301 cgcgtcgcgt ggtggacggt cgggctgttg ctgatgttcc cggccggatt cttcttctcg
      361 gccgtcttcc cagaatcgct gctgctgctg ggaatcgccg ctgcgcttta ccacgcgcgg
      421 cgtgggcagt ggtggcgcgc cggtctgttt gggctgctgg ccggcgcgac cggcctggcc
      481 gggatgctga tcgtcgtccc gctggcgttg gagctctggc gacaggaagg tggacgacgc
      541 atcgacgtcc gagacatcgt cgcggtcgcc attgccccgc tgggcgggct ggcgtacttc
      601 gggtatctct gggcgcgttt cggcagcccg ctggtctact tcgacagcat cgccgcctat
      661 cggcgtgaca tccgcgcgtt cgacagctac cggaacggca ctggcggctc gctcgacaac
      721 gtcttcgctg tctccgacgc ggccttgatc ggcgtctttc tgatcgccgg tgcgctgctg
      781 tggtggcggg tgcgaccgag ctatggcgcg ttggtcatcg gactcacgct gctcccgctc
      841 gtcgttggcg cgccgccggg catcgggcgg cagctggtcg tggcgtttcc ggccttcatg
      901 ttgctggggc gcatccccag cgaaggagta cggatgatgc tcagcgccgt cttctcactc
      961 ggcctgatgc tgacggcgtt cttcttcgtt cagggattct gggcgggcta accggcgacc
     1021 ctgctcgatg cgtcgccggt cagcccggtt gcctcagttg tctcgcatgc ccggaccgcg
     1081 caccattcgg aagtccgcca gccgcggccc cttcttcttg ccgttggcct tttcgttcgt
     1141 atccttgatg accgggtgaa caccgagcca gcggcggtgc tggcggttga cgccacgggc
     1201 tgccagcgcc ttgatgagct ggtcgtcggc aatcgcccga aactgcatgt cgcgcagggc
     1261 gtccagcccc gccagcccca ggcactcgcc aaactccagc gcgcggtcgt agctcgtgct
     1321 gcgggcggca gcagcggcaa atgccggatc gccgttcacg acctgatcgg tcgtgaacgg
     1381 gaagctcagc cggaaggccg gcgtcacctg cgagacgttg ccccaaccgg tcgctggctc
     1441 cggatcgagc ttcgcgacct gctcaaacgt atagccgagc gtgtcggcgt aggtcttgac
     1501 gcgccgcgcg aggatccggc tgacgagcat gtcgtcggtc ggattcccga agctgacact
     1561 tgcgccgggc gtctcagcgg ccagccgctg gacgatcgcg ctcagatcac gcaactcgac
     1621 gctcgtccgc gcagtcagcg tcagcctgac gaagccatcc tcgatcgagc catcgatccg
     1681 actcggggcg tcatgctgcg ccgtctcagc ttcgagctga gccaggaatg cggttgcgtc
     1741 tcgttcgacg gtgatgtcgg ccgaacggct ggcgagcgtg tcaccggtcg aattgatcgt
     1801 gtaggcgaag ccggcgccag tctccgcagg cctggcaccg aagacggcgt cgaactcgtc
     1861 gaagacgccg gcgttagcga tcgcaagcag cgcggcgtcg ccgggatagg tgataacggt
     1921 gacgagacca tgctcggatt ccagcgcgac gccgcagcca atcgctgccg ccagtacggt
     1981 tgccgatccg agagacgtgg ggctatcgag atcgacgagg taagcgatat ggccgtgtcg
     2041 cagcccattg cgcatcgccg cccggtcgag gtgcttccga tcggcgcggc tggcgtctgc
     2101 gataccggcc acgccggatg aaacctcaaa gccgtgaccg gcgagcagtt cattcaaccg
     2161 ctcggccttg cccgagtcgg tctcaatatc acggcaggcg cgtgtgacct cgatgcgcgc
     2221 tgcccgaatg gccgcgattg tagcttcgcg ggccgtgtcc ttgaccggca tgcggaatcc
     2281 tccctggttc agcgaacagg gctgaattgt acgcaaccgg cgactattcg cgcctagccg
     2341 gcaacgaaac gcagccgcgc gttgccggcc tgtgccggat cgatgtcacg cgtctcgcgg
     2401 gccagcagcg acagctcgaa cgggctatcg cccctcgcgc gcagttgatc ggccagcggg
     2461 ccgtcgccgg tcggcgttgc cagatcgatc cgatgctcgc cgaccatgaa gtaaacgccg
     2521 tcggcgttgt cgagattcgg aagtccctcg ccggatggct tctcccggtc gagcagcgtg
     2581 cagtagcgct tgacggccgc atccagatcc tgaacggcga cgaccagcga gcggatgcca
     2641 cggacagtgt tgtcatgcgt tgcgctctca ctcggcacgc gcaacccgcg atcggtcacg
     2701 tcctcgatca ggaacggcaa cgcgccgacg ttcgggtcgc ccttcgccat tgagctcttc
     2761 caggcgatct cgacgtcgtc cagtcgcttg cgtgcgccgg gcgtcgctgc accgtgggtg
     2821 tatccgcgcg tatcggcatc ggccaccagc gtgtcgaggt tcggagcggc cagcgcatag
     2881 tcgatgatgc cgccgccctt gccgacgtgc ttgaaccaac gatgtccttc gccggggttg
     2941 gtcgacgtcg gttgctcgta gaacgcgatc agctccaggt agctgccatc tgcgaaggtc
     3001 accagcgcgt tgtgggtgta gcgcgggtgc ttgccgccgg gtgtaacggt gaagcccagc
     3061 ttccgatatt gctcgatccc gcgatccaga tcgtctacga gaatgatgac gtggtcgatt
     3121 ccagttggca tccggtggac tccctctccg tctcgcggct gcatgccggt cagcaaattc
     3181 gcccgaccgc cgattccggc gcggtcatcg cgattgtaaa cctgacgcgc gcgtcacggt
     3241 ttggcaccac acggcacgat tggtgccggg cgcgcgaccg ctcgcgcgat gttcgttaca
     3301 atcacgctcg gagaccacgc agactggaca ggggaacgat gccggcaggt tggccacacc
     3361 accgaacacc acgccagatc gcgcgatcta tcgctctcat cacactggct ctcgccctgg
     3421 ccaccgcctg cgctggtccg gaaaccggcg atgatcgcga cccgactccg acaccaaccc
     3481 agacagcaac accgaccgaa gtgccaccaa ccgcgacgac ggccgcatct ccaacggccg
     3541 cctcgaccga cactcccgtt ccgacgacct ccgcgtcgcc gacaaaggcc gcgtcaccgt
     3601 cggtggcggc atcaccaact ccgacggtcg ttccgctgga cgaacggctc ccgctgctcg
     3661 acgacatgcc cggcgcgggc tacacgattg cggaggaggg aacgcgtacc gccgaggaac
     3721 tggccgaggc ctacgccgat ccggccgccc acttgcgccg gctcgacgaa tggggcttca
     3781 agcgccacat cttccgggcg tttctgctga ccgacagcga cgacaaccgc ctgccaccca
     3841 acatcctggc gacgatcaac ctctacggca gcgacgagca ggccgaggag gccctgcagt
     3901 ggctgcgtca gcttggaacg gcgaccggcg cgaccgacgc ggagccgccg ccgatcggcg
     3961 acaacgcggt cgcgttgacc gtcccaacct cggatggcgt cccaaccgca tccatctacg
     4021 ttcgccaggg agcgatcctg tacgtctact tcgccgaggg cggcgatcct ctaccggccg
     4081 tcgacagcat cgcgaccaac gtcttcagcc gctgatcctc gtccctgctc tgtgggatcg
     4141 acgaccaaac ctgtaccaaa tcgcctacca tcactcatcg gcccaggcgt atactttgtg
     4201 aaggagatca caagtgtgtt ccccgctcgg tcgcgagcgg gtgagacaag gggtccaaaa
     4261 ccatgacaga caacgcgcag tcgtcagagc acggagcaac ggagctccgc aacgtacgca
     4321 tcctccggtc gaaggggctg ctggcggagt acacgaccgg ccaggcgcca tcgggtgagt
     4381 ggttcgcgct cggctccgtc cgtagcgagc tgccgctgac cagcccgccg gcctggatcc
     4441 tggtcggcac cggcggctcg gt
//
LOCUS       DASUGJ010000488         3664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1882134, whole genome shotgun sequence.
ACCESSION   DASUGJ010000488 DASUGJ010000000
VERSION     DASUGJ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882134"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VFV93_10905"
     CDS             <1..1192
                     /locus_tag="VFV93_10905"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX5165894.1"
                     /translation="AGHPIAYGEWLGAGPGKPTVLVYGHYDVVPASIEDGWDTEPFEP
                     VIKDGKIYARGATDDKGQLMIHVNAFESYMKTGDGPPVNLKFLLEGEEEVSSPNLRPF
                     LESHLDLLQADVCVISDSSMPTIDEPAILHSLRGMTYVEIEVEGPHDDLHSGFYGGAV
                     HNPALALVEILSKLHHPDHSIAVPGFYDDVVGLTDEERAEVAKTGISDEQLRQATGVP
                     AAWGEAAYTIKERVTARPTLEINGIGSGWTGPGPKTIIPATAMAKVSCRLVGNQDPHK
                     IYEQIIAYVESLTPDTVRVAVNLITTGEPALIDFTIPEMQAAARAYEKAWGARPAFTR
                     GGGSIPIVADFSNLMQMPVVMMGYGLDTDGLHGTNEHFTLEMFYRGIETSIVYLEELA
                     QLSR"
     gene            1539..2864
                     /gene="pepT"
                     /locus_tag="VFV93_10910"
     CDS             1539..2864
                     /gene="pepT"
                     /locus_tag="VFV93_10910"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF009920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEX5165895.1"
                     /translation="MTEAFDRELEERTVRYCLIDSQSDEASATTPSTAIQYDVLNLLV
                     NELNEIGAQDVTLTDYGAVIATIPATVDADVPTVAFLAHVDTAPAFNATGVKPIVHRA
                     YAGGDIVLPDDPSQVLSPNELPYLASKAGDDIITASGTTLLGADDKAGVAIIMVMARH
                     LIEHPELKHGPIRVCFTPDEEIGRSVHASLPADLRADVAYTLDGAGLGEIVYETFSAD
                     KATVTIQGVSAHPGQAKGVLVNALHLAAKIIDTLPHVTRTPETTDDRQGFIHAYLMTG
                     TAAQVELHLILRDFERDGLEAHGELVRQVCAAIQAGEPRATITCEITPQYRNMRYWLE
                     DDMRPVELARVACRQLDIAHFSTPTRGGTDGSRLTELGVPTPNLFTGMQNIHGPLEWV
                     SVQDMRRATEMCIRLSELWGKEQPTGRREDRIAERESPTDEGSAVDWTG"
     gene            2989..>3664
                     /locus_tag="VFV93_10915"
     CDS             2989..>3664
                     /locus_tag="VFV93_10915"
                     /inference="COORDINATES: protein motif:HMM:NF015746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEX5165896.1"
                     /translation="MSATAPPLDAVPQKGFVQKMLDGIEKAGNKVPHPVMMFLYLILL
                     VIALSVILDLLNVGVTEDVLVPVTEQDKIELTIAGGSREMITMPGENGEDIYFVIEEQ
                     TTNVRSLLSISGIRFIFSSFVSNFAGFSVVAVIFVARVGVGVAEEAGLMGALIRKLVA
                     IAPAGLLTFSIVLIGGLSSVASDAGYLILIPLGAAAFLSVGRHPLAGMAAAYAGVAAS
                     FSVNVLI"
BASE COUNT          705 a         1167 c         1175 g          617 t
ORIGIN      
        1 ggccgggcat ccgatcgcct acggtgaatg gcttggagcc ggccccggca agccgacggt
       61 gctggtctac ggacactacg atgtcgtgcc ggcctcgatc gaggatggct gggataccga
      121 gccgttcgag ccggtcatca aggacggcaa gatctacgcg cgcggcgcga ctgacgacaa
      181 gggccagttg atgatccacg tcaacgcctt cgagtcctat atgaagaccg gtgacggccc
      241 gcccgtcaat ctgaagttcc tgctcgaagg ggaagaagag gtctcgtcgc cgaacctgcg
      301 gccattcctc gaatcgcatc tcgacctgtt gcaggccgat gtctgcgtca tcagcgactc
      361 gtcgatgcca actatcgacg agccggccat tctgcatagc ctgcgcggca tgacctacgt
      421 tgagatcgag gtcgaaggcc cgcacgacga tctgcacagc ggcttctacg gcggcgccgt
      481 ccacaacccg gccctcgcgc tggtcgagat cctgtcgaag ttgcaccatc cggaccacag
      541 catcgctgtg cccggcttct acgacgatgt cgtcgggctg accgacgagg agcgcgccga
      601 ggtcgccaag acgggtatca gcgacgagca gctcaggcag gcaaccggcg tcccggccgc
      661 ctggggcgaa gccgcctaca cgatcaagga acgcgtcact gcccggccga cgctggagat
      721 caacggcatc ggcagcggct ggaccgggcc ggggccgaag acgatcatcc cggcgaccgc
      781 gatggccaag gttagttgcc ggctggtcgg caatcaggat ccacacaaga tctacgagca
      841 gatcatagcc tacgtcgagt cgctcacacc cgacacggtc cgggtcgccg tcaacctcat
      901 cacgaccggc gaaccggcgt tgatcgactt cacgatcccc gagatgcagg cggcggctcg
      961 cgcctacgag aaggcgtggg gcgccagacc ggcatttact cggggcggcg gcagcatccc
     1021 gatcgtggcc gacttctcca acctgatgca gatgccggtt gtcatgatgg gctacgggct
     1081 ggacaccgac ggcctgcatg gcaccaatga gcacttcacg ctcgagatgt tctatcgcgg
     1141 tatcgagaca tccatcgtct atctggagga actggcgcag ttatcacgct gatgcgtcgt
     1201 cgttgtgacg tccgcccggg cagtacgcgt tgttccagtc tggagcagag atccgaatgg
     1261 catcgtcaaa cgactcacaa cccagccacg gggccagcgc cagtgcggca actgtcgatg
     1321 gcgtgccgat ttcgacagga cggttgccac tccgtgagcg cgatcagtgg gatggcgacg
     1381 cttgctccag cgctggatgc agccggcaac agcgtcaaag gactgattgc cccgcggttt
     1441 ctctccgaac agttggggct gaatgccttt gcctcgaaac cacctgaatc atcgcgacat
     1501 gcgaccggag acgtggtcgg ccgagaagga ggaggaacat gactgaggca ttcgaccggg
     1561 agcttgagga gcgcaccgtg cgctactgcc tcatcgactc ccagagcgac gaggcgtctg
     1621 cgacaacgcc gagtacggcg atccagtacg acgtgctcaa cctgctggtc aacgagctca
     1681 acgagattgg cgcacaggac gtgacgctga ctgactatgg cgcggtcatc gcgacgatcc
     1741 cggcaaccgt cgatgccgac gtcccaaccg tcgcgtttct ggcgcacgtc gacaccgcgc
     1801 cggccttcaa cgcgaccggc gtcaagccaa tcgtccaccg cgcctacgct ggtggtgaca
     1861 tcgttctgcc ggacgatccg agccaggtgc tctcgccgaa cgagctgccg tatctggcaa
     1921 gtaaggccgg cgacgacatc atcaccgcca gcgggacgac gctgctcggc gccgacgaca
     1981 aggccggcgt cgccatcatc atggtcatgg cgcgacacct gatcgagcat cccgaactga
     2041 aacacggtcc aatccgggtc tgtttcacgc cggacgagga aatcggccgt agcgtccatg
     2101 ccagcctgcc cgctgacctc agggccgacg tcgcctacac gctcgacggg gcagggcttg
     2161 gcgagatcgt ctacgagacc ttctcggccg acaaggcgac cgtcacgatc cagggtgtct
     2221 cggcccatcc cggccaggca aagggcgtac tcgtcaacgc gctccacctt gcggcgaaga
     2281 tcatcgacac gctcccgcat gtcacgcgga cgcctgagac gaccgatgac cgccagggct
     2341 tcatccacgc ctacctgatg accgggacgg cggcccaggt cgagctgcac ctcatcctgc
     2401 gcgacttcga gcgggatggt ctggaagcac atggcgagct cgtccggcag gtctgcgcgg
     2461 cgatccaggc cggtgagcca cgcgcgacga tcacctgcga gatcaccccg caataccgca
     2521 acatgcgtta ctggctcgaa gacgacatgc ggccggtcga gctggcccgt gtggcgtgcc
     2581 ggcagctcga catcgcgcac ttctcgacgc cgacacgcgg cgggaccgac ggctcgcggc
     2641 tgaccgaact gggcgtcccg acgccgaacc tgttcaccgg catgcagaac attcacggcc
     2701 cgctggaatg ggtcagcgtc caggacatgc gacgcgcgac cgagatgtgc atcaggttgt
     2761 ccgaactgtg gggcaaggaa caaccgaccg gccggcggga ggaccgcatc gcggagcggg
     2821 agtcacccac cgacgaaggg agcgccgtcg actggaccgg ttgaccgaca cgggggcagt
     2881 ggcggcggtc tctgccgccg gcaggaagac cgacatcaac gttgagggat gtttccagcc
     2941 agcacaggtt gagacgtgcg aactggttag agaaggggat ggggcaacat gagcgcaaca
     3001 gcacccccat tggacgcagt tcctcagaag gggttcgtcc agaagatgct ggacgggatc
     3061 gagaaagcgg gaaacaaggt cccgcatcca gtcatgatgt tcctgtatct gattctgttg
     3121 gtcatcgcgc tctcggtgat cctcgacctg ctgaacgtcg gggtaacgga agacgtcctc
     3181 gtccccgtca ctgagcagga caagatcgag ttgaccatcg ccggcgggtc cagagagatg
     3241 atcacgatgc ccggcgagaa tggtgaggac atctacttcg tcatcgagga gcaaacgacg
     3301 aacgttcgca gtctgctctc gatcagtggg attcgcttca tcttctcatc gttcgtgagc
     3361 aacttcgccg ggttcagcgt cgtcgccgtc atctttgtcg cgagggttgg cgtcggcgtc
     3421 gccgaagagg ccgggctgat gggcgcgttg atccgcaagc tggtcgcgat tgcgccggct
     3481 ggcctgctga ccttcagcat cgtcctgatt ggcgggctgt cgagcgtcgc gtcagacgcg
     3541 ggctacctga ttctgattcc gctcggagcg gcggcgttcc tgagcgtcgg tcggcacccg
     3601 ctggcaggca tggcggcagc ctacgctggc gtcgctgcgt ccttctcggt caacgtcctg
     3661 atcg
//
LOCUS       DASUGJ010000489         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1049479, whole genome shotgun sequence.
ACCESSION   DASUGJ010000489 DASUGJ010000000
VERSION     DASUGJ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049479"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFV93_10920"
     CDS             complement(<1..309)
                     /locus_tag="VFV93_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5165897.1"
                     /translation="MDFAADPAEVTMTEDDPEAPQHYGLDPAEREAILSQFAALYPFP
                     LDQFQLEAGRAFLDGESVMVAAPTGTGKTILAELGVYEAFRRQGRVIYTTPIKALSNQ
                     K"
     gene            complement(486..833)
                     /locus_tag="VFV93_10925"
     CDS             complement(486..833)
                     /locus_tag="VFV93_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX5165898.1"
                     /translation="MQPRSLKSVEDIANACSNIVEWTVGGSLAQHESDSKLRAAVERT
                     FEIIGEALLRLERNDPTLVARITDYRRVIGFRNRLAHGYDDIDDSQVWDIIQTSLPVL
                     RQDIDTILAEIER"
     gene            complement(805..1137)
                     /locus_tag="VFV93_10930"
     CDS             complement(805..1137)
                     /locus_tag="VFV93_10930"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX5165899.1"
                     /translation="MPPLIEQHLTRIRALAHDYGVTRLDVFGSACTPAFNPERSDIDF
                     LVEYPAGYDYGPWLARVQDLEADLSNLLGRKVHLVMTAALRNPRFRSEAEKTRTVIYD
                     ATQVAQVG"
     gene            1221..1754
                     /locus_tag="VFV93_10935"
     CDS             1221..1754
                     /locus_tag="VFV93_10935"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5165900.1"
                     /translation="MPDGRRTLIAEPPAGYPPEIARWLWVFADTRRRTLEVLEGVDEA
                     RLDEPGPGGNSIATILAHIALIETDWLYVEILGEEYPADLIALLPPEARDGEGQLMAT
                     PGLPLARYLAALSAVRAKLVERVGALSPADLDRLRSLPEYDVSPAWVLNHLIQHEAEH
                     RAEISITRAVLEGSRYQ"
     gene            1840..>2202
                     /locus_tag="VFV93_10940"
     CDS             1840..>2202
                     /locus_tag="VFV93_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165901.1"
                     /translation="MEEQAQIILDLTNTPNWLSKRITPQRDMSRVNRPAARGEWSARD
                     IIAHLRDNEARYFPKLHLISIAQVPDLRRVQQIGPTEYDPNDSIFTVMSQFRRIRQST
                     LSLLRELPADGWKRVGVDV"
BASE COUNT          370 a          692 c          739 g          401 t
ORIGIN      
        1 cttctggttg gagagcgcct tgatcggggt cgtgtagatg acgcggccct gccgccggaa
       61 cgcctcgtag acgcccagct cggccaggat cgtcttgccg gtcccggtcg gcgcggcaac
      121 catgaccgat tcgccatcga ggaaggcgcg gccggcctcc agctgaaact gatcgagcgg
      181 gaatgggtag agcgcggcga actggctgag gatcgcctca cgttcggcgg ggtcgagtcc
      241 gtagtgctgt ggcgcttcag ggtcatcctc ggtcatcgtg acctcggcgg ggtcggctgc
      301 aaagtccatt gctgttccaa ctgtcctgga cgctcgcggc cgtgggtccg atctggtggg
      361 ccggcggcgt cactactgac actgtcccgc gcagaaaccg cgccgggacg ggcgattatt
      421 gggccggagt ataccgaaca catcgtaggt ccggtttggt gactgaccgc agtggtggga
      481 tgcgcttacc gctcgatctc ggcgaggatc gtgtcgatgt cctggcgcaa caccggcaag
      541 gaagtctgaa taatgtccca tacctggctg tcgtcgatgt catcgtagcc gtgtgccaac
      601 cggtttcgga agccgatgac gcgtcgatag tcggtgatac gcgccaccag cgtcggatcg
      661 ttccgctcca gccggagcag cgcttcaccg atgatttcga aggttcgctc gacggctgca
      721 cgcaatttgg aatcggactc gtgttgagca aggctgccgc caacggtcca ttcgacgatg
      781 ttcgagcaag cgttggcgat gtcctcaacc gacttgagcg acctgggttg catcgtagat
      841 gaccgtccgt gtcttctcgg cttcgctgcg gaatcgcgga ttccgcagcg cggctgtcat
      901 tacgaggtgg accttgcgac caagcaggtt gctgaggtcg gcctccaggt cctggacgcg
      961 ggccagccac ggaccgtagt catagccggc cgggtattcc acaagaaagt cgatgtcgct
     1021 gcgctccgga ttgaatgccg gtgtacaggc cgaaccgaac acatccaggc gcgtgacgcc
     1081 gtagtcgtgc gcgagggcac gaatccgtgt gagatgctgt tcgatcagtg gaggcatggc
     1141 tgctgcttcc tcaatgtgct gttgcgtatc cttgtcgtgc tctccatcga ccgtcagtat
     1201 agcgaaggag cacagcccgg atgcctgatg gacgccgaac gctgatcgcc gagccgcctg
     1261 ccggttaccc gccggagatc gcccgctggt tgtgggtctt cgccgacacg cggcggcgga
     1321 cgctggaggt gctggagggg gttgacgagg cgcggctcga cgagccgggg ccgggtggca
     1381 actcgatcgc gacgatcctg gcccacatcg cgctgatcga gaccgactgg ctgtacgtcg
     1441 agattcttgg cgaggagtac ccggccgatc tgatcgcgct gctgccgccg gaggcgcgcg
     1501 atggcgaggg ccaactgatg gcgacgcccg gcctgccgct ggcgcgctac ctggcggcgc
     1561 tgtcggccgt ccgcgcgaag ctggtcgagc gggtcggcgc gctctcgccg gccgacctcg
     1621 accgtcttcg cagcctgccg gagtacgatg tctcgcccgc ctgggtgctg aatcacctca
     1681 tccagcacga agccgagcac cgcgccgaga tctccatcac ccgcgccgtc ctcgaaggca
     1741 gccggtacca gtagagcgtg gagccccaca tcccgcgtcc cgcccgactc gcagtacaat
     1801 accgctgacg ggtccggcat cgacgggaag atgggggaga tggaagagca agcgcagatc
     1861 attctggatc tgacgaacac gccgaactgg ttgtcgaagc gcatcacgcc gcagcgtgac
     1921 atgtcgcggg tcaaccgtcc ggcggcgcgc ggcgagtggt cggcgcgcga catcatcgcc
     1981 cacctgcgcg acaacgaggc gcgctatttc cccaagctgc acctgatctc gatcgcgcag
     2041 gtgccggatc tgcgccgtgt tcagcagatc ggcccgaccg agtatgatcc gaacgactcg
     2101 atcttcaccg tcatgagcca gttccggcgc atccgccaga gcacgctctc gctgctgcgc
     2161 gaactgccgg ccgacggctg gaagcgagtc ggcgtcgatg tc
//
LOCUS       DASUGJ010000490         3925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_772114, whole genome shotgun sequence.
ACCESSION   DASUGJ010000490 DASUGJ010000000
VERSION     DASUGJ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772114"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..675
                     /locus_tag="VFV93_10945"
     CDS             136..675
                     /locus_tag="VFV93_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5165902.1"
                     /translation="MTEGIVVRDEEIIQRSMGLVPEIARRMHATFIGHPLAAGRPIGQ
                     AKLMGLLYRVGRCTVGEAAVGLGVTMPTASEQIDRLVEEGLLVREVNPADRRQVLVDL
                     TPKAREFGRELHELRLRQLRAALDRLEPDDRPVFLRSLEALVDALRLAPDELPPAPAG
                     FEQPDACPAASSPPRQGTA"
     gene            747..2405
                     /locus_tag="VFV93_10950"
     CDS             747..2405
                     /locus_tag="VFV93_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5165903.1"
                     /translation="MTEQALSGQQRGRPDDDAMMRQGGSQNPWLVLIVLCAAIFMLLL
                     DTTIVNVAQREIQIGLGADLSEIQWVLDSYILTYAVLLLAFGRMGDIFGRKRLFVIGM
                     SLFTAASALCGASSWLGDLVGVSGVNALIAARVLQGLGGAFMMPQTLSLITVVFPAEK
                     RGAAMGIWGGIVALGAVVGPIIGGLIVTNYAWEWVFLINIPVGIVAVIATLAIVPESV
                     DPLASRKIDWGGVILSGFGIFGIVFALIEGNNWGWTDPAIVGLFVVGLVLLAVFVWWE
                     SRAVDPIMKIELFRLRNFWVGNVIAFVVAFGMLGIFFPMTLFLQGVLGMTPIRAGLTM
                     TPMSVMIMFAAPMAGRLSDKIGARWILTSGLLLMTGGILFIISRIDLETDWKSLAPAL
                     IVTGLGMGMTFAPMTAAAMREVPPRIAGSASGILNTTRNVGQVLGIAVLGSLLQSEVA
                     SHTRTELESVPLDSGVRDQIVDLAKASRFEEIAGAVPQPELVPAVFSAIQNAFVDSIH
                     ITFLVGSAACLIGALTALAIRDIKAPTAERHREALERATVPGAD"
     gene            2535..3830
                     /locus_tag="VFV93_10955"
     CDS             2535..3830
                     /locus_tag="VFV93_10955"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5165904.1"
                     /translation="MTEQQPASSSRKRSRLPKGLAAFKHRNYRLFYFGQLISVTGTWM
                     QTLAQSWLVLSLTSSAFLLGLVSVFQFAPMLVLGLFAGLVADRMPKRKLLVGTQAISA
                     VLAGTLAILTFTDAIQLWQVYLLAFLLGVNNAFDMPTRQAFVVEMVGKEDLTNAIALN
                     SSIFNAARIVGPGIAGALLAAFGPAVCFAINSVSYIAVISGLLMMRIAPLARVHISQR
                     RLEQIREGLQYVRRTPEILRPVLLVGFVATFGMNTNIWVPLLAKNDLDSGASGFGLLM
                     SSMGVGALTGALGLAFLVSSVKRWMLFSTAGSLGVALLFLALAGAIPLAIPIAMFVMA
                     IIGLFSTTTMALANTTVQSASPDELRGRIMSVYTTVFTGTAPFGALVASAIANRFGTP
                     VSLLIGGIVTLLAVAVVLLTGGAKLAFRERMRLPNRRRV"
BASE COUNT          579 a         1247 c         1333 g          766 t
ORIGIN      
        1 catctatcgt aacgcatgac atccgtcgcc ggtagtgggc caggacacgt gacaagcaga
       61 cgatcctgtc cggacgatca ggttgacacc cgtcgatcct tcggtcatca tattgttcgc
      121 taaccgaagt attcgttgac cgaagggatc gttgtgcggg acgaggagat catccagcgt
      181 tcgatggggc tggtgccgga gattgcgcgg cggatgcacg ccacgttcat cggacacccg
      241 ctggcggccg gccggccgat cgggcaagcc aagctgatgg ggctgctcta ccgcgtcgga
      301 cgttgcacgg tcggcgaggc ggcggtcggg ctgggtgtga cgatgccgac ggcgtcggaa
      361 cagattgaca ggctggttga agaggggctc ctcgtccgcg aggtcaatcc ggccgaccgt
      421 cgtcaggtgc tggtggatct cacgccgaag gcgcgcgagt tcggccgcga actgcatgag
      481 ttacgcctgc gacagctgcg ggccgcgctc gaccggctcg aaccggacga ccggccggtg
      541 ttcctacgct cactggaagc cctcgtcgac gcgttgcggc tcgctccgga cgaactaccg
      601 cccgcgccgg cgggcttcga gcagccggac gcatgtcccg ccgcgtcgtc accaccccgc
      661 caggggaccg cttgaattcg acgaccaatc cacgtcgcca gtaggaagcc gtgatcgctg
      721 atgacacggc cggggaaggt attgatatga ccgagcaggc actgtccggg cagcagcggg
      781 gccgtccgga cgatgacgcg atgatgcggc agggcggcag tcagaatccc tggctggtgc
      841 tgattgtcct ctgcgccgcg atcttcatgc tgctgctgga cacgacaatc gtcaacgttg
      901 cccagcgcga gatccagatc ggcctcggcg ctgacctgtc ggagatccag tgggtgttgg
      961 actcgtacat cctgacctat gccgtcctgc tgctggcctt cggccggatg ggcgacatct
     1021 tcggccgcaa gcggctgttc gtgatcggga tgtcgctctt cacggccgcc tcggcgctgt
     1081 gtggcgcctc cagctggctg ggggatctgg tcggcgtttc cggcgtcaac gcgctgatcg
     1141 ccgcccgggt gttgcagggg cttggcggtg cgttcatgat gccgcagacg ctctcgctga
     1201 tcacggtcgt cttcccggcc gagaagcgcg gcgcggcgat gggtatctgg ggcgggatcg
     1261 tcgcgctcgg cgccgtcgtc gggccgatca tcggcggcct gatcgtgacc aactacgcct
     1321 gggaatgggt cttcctgatc aacatcccgg tcggcatcgt cgccgtcatc gcgacgctgg
     1381 cgattgtgcc ggaatcggtt gatccgctgg ccagccgcaa gatcgactgg ggcggtgtca
     1441 tcctctccgg cttcggtatc ttcgggatcg tcttcgcctt gatcgagggc aacaactggg
     1501 gctggaccga cccggccatc gtcggtctct tcgtggtcgg gctggtgctg ctggccgtct
     1561 tcgtctggtg ggagagccgg gccgttgacc cgatcatgaa gatcgagctg ttccggttgc
     1621 ggaacttctg ggttggcaac gttatcgcct tcgttgtcgc cttcgggatg ctcggcatat
     1681 tcttcccgat gacgctcttc ctgcagggcg tgctggggat gacgccgatc cgggccggtc
     1741 tgacgatgac gccgatgtcg gtcatgatca tgttcgccgc gccgatggcc gggcggctgt
     1801 cggacaagat cggcgcgcgc tggatcctga ccagcgggct gctgctgatg acgggtggga
     1861 ttctgttcat catcagccgg atcgatctcg agaccgactg gaagtcgctg gcgccggccc
     1921 tgatcgtgac cgggctgggg atgggcatga cgttcgcgcc gatgacggct gccgccatgc
     1981 gcgaggtgcc gccacggatc gccggcagcg catccggcat cctcaacacg acccgcaacg
     2041 tcggtcaggt gcttgggatc gccgtcctcg gttcgctgtt gcagtcggag gttgcctcac
     2101 atacccggac ggagctggag tcggttccgc tcgacagcgg cgtccgcgac cagatcgtcg
     2161 acctggcgaa ggccagccgc ttcgaggaga tcgccggcgc tgtcccgcag cccgaactgg
     2221 tgccggccgt cttcagcgcg atccagaacg cgtttgtcga cagcatccac atcacgttcc
     2281 tggttggctc ggccgcctgc ctgatcggcg cgctgacagc gctcgcgatc cgtgatatca
     2341 aggcgccaac agccgagcgt caccgcgagg cgctggaacg ggcgacggtg ccgggagcgg
     2401 attgagacga tcgggattcg ggaaccggga ttcgggggta tcaaacggaa catgtggctc
     2461 tcgatactcc cgagtcccca tcccgaatcc cgacgtcacg tcgtgtacga tgacgcggtg
     2521 acgcagggag gggtatgacc gagcagcagc ccgcttcatc atcacgcaaa cggtcccggt
     2581 tgccgaaggg gctggcggcc ttcaagcacc gcaattaccg gctgttctac ttcggtcagc
     2641 ttatctcggt gaccgggacc tggatgcaga cgctggcgca atcgtggctc gtgttgtcac
     2701 tgacgtcgtc ggcgttcttg cttgggctcg tctcggtctt ccagttcgcg ccgatgctgg
     2761 tgctggggct gttcgccggc ctggttgccg accggatgcc gaagcgcaag ctgctggtcg
     2821 gcacgcaagc gatctcggcc gtcctggctg ggacgctggc gatcctgacc ttcaccgatg
     2881 cgatccagct ctggcaggtc tacctgctcg ccttcctgct gggcgtcaac aacgcgttcg
     2941 acatgccaac ccgccaggcg ttcgtcgtcg agatggttgg caaggaggat ctgaccaacg
     3001 cgatcgcgct caactcatcg atcttcaacg cggcccgcat cgtcggtccc ggcatcgccg
     3061 gcgcgctgct ggcggcattc gggccggccg tctgcttcgc gatcaactcg gtctcgtaca
     3121 tcgccgtcat cagcggcctg ctgatgatgc ggatcgcccc gctggcacgg gtccatatca
     3181 gccagcgccg gctggagcag atccgtgagg ggctgcagta cgtgcgccgg acgccggaga
     3241 tcctgcggcc ggtcctgctg gtcgggttcg tcgccacgtt cgggatgaac accaacatct
     3301 gggtgccgct gctggccaag aatgacctgg acagcggcgc gagcggcttc ggtctgctga
     3361 tgtcgtcgat gggcgtcggc gcgctgactg gcgcgctggg gctggcgttc ctcgtctcgt
     3421 cggtcaaacg ctggatgcta ttcagcacgg ccgggtcgct tggcgttgcg ttgctgttcc
     3481 tggcgctggc cggtgcgatc ccgctggcga tcccgatcgc gatgttcgtg atggcgatca
     3541 tcggcctgtt cagcacaacg acgatggcgc tcgccaacac gaccgtccag tcggcctcgc
     3601 ccgacgagct gcgcggccgg atcatgagcg tctacacgac cgtcttcacc gggacggccc
     3661 cgttcggcgc gctggtcgcc agcgcgatcg ccaaccgctt cggcacgccc gtctcactcc
     3721 tgatcggcgg catcgtcacg ctgctggccg tcgccgtcgt cctcctgacc ggcggcgcaa
     3781 agctggcctt ccgcgaacgg atgcggcttc cgaatcgccg gcgggtgtag ccctgttctc
     3841 gcgtaggggc gatggccgca gccacctgtt ccaacttaat cggatgaacg gcgttggcac
     3901 ttgcgtttgg catagcgtgg ggtcc
//
LOCUS       DASUGJ010000491        10693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_217313, whole genome shotgun sequence.
ACCESSION   DASUGJ010000491 DASUGJ010000000
VERSION     DASUGJ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217313"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VFV93_10960"
     CDS             complement(<1..448)
                     /locus_tag="VFV93_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165905.1"
                     /translation="MQRTPQPHRFSIVLLLLALLLAGCIGSAGVSEPDGLLATVLTEP
                     PPIRLTVTPGIATSIPSTATSTPTDPAAGLTATATVTPLPASTSTATPVDPTATMSPT
                     PSGAGDATPVIVLDPGHDRMFPSALGIEYQVNLRTALIAREALEAAG"
     gene            complement(520..942)
                     /locus_tag="VFV93_10965"
     CDS             complement(520..942)
                     /locus_tag="VFV93_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEX5165906.1"
                     /translation="MKRVVFDTVIIVRGLINPYGQWGRIVFDRANDYQQVVSPSIVAE
                     YLEVIRRPELVRKYRSVDTRDPHAVLTWLAQAEVVEYEDVPAVSRDPKDDPFLATATV
                     GNVDLLVSEDRDLLDRDSYEDIPIVTGSMFLKILDAGP"
     gene            complement(939..1259)
                     /locus_tag="VFV93_10970"
     CDS             complement(939..1259)
                     /locus_tag="VFV93_10970"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX5165907.1"
                     /translation="MSESTTMTKLVRPLRGGQITIPAEFRKRLGITDETMLQVRLDDG
                     ELRIRPVRLSDATEGSPWLRELYAYFAPVRQEIIERNIPEEEVNADIDAAIKAARASR
                     RSSR"
     gene            1517..2695
                     /locus_tag="VFV93_10975"
     CDS             1517..2695
                     /locus_tag="VFV93_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5165908.1"
                     /translation="MASDPEVTTGTGGLFELDDVHREFQQVCRDFVNEQIMPKVERAE
                     REGQFPLELMPLMGRYGFLGLGLPEDAGGTGGDMLAIAILSEELGRSSGGIAVTPLAS
                     AYMAAPHLAKYGTPDQQQRYLLPITTGEKIASIGVTEPGAGSDVAGMRTTARETTGGY
                     LINGTKLFITNGGFADYIVLGAKTDPTERHRGITMFLIEKDDPGFSLGRPLQKMGWHS
                     SDTRELIFEDCFIPADRVIGEEGRGFYQIASSFQTERITLSGMGVGLAQAAFDDALQY
                     AKDREAFGQAIGKYQAIRHMLAAMATDIQTGRLLLYQAAAKLDSNAPDALDVVAMAKY
                     HTAIIANTVADRAVQIFGGMGYIEETRVAMHYRDARILRIGGGTDEIQLEILAKRMGL
                     "
     gene            3252..4325
                     /locus_tag="VFV93_10980"
     CDS             3252..4325
                     /locus_tag="VFV93_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165909.1"
                     /translation="MPSWPIVVALDPDGGEQYKLHLIETDGALRRLTPQDSEIIGAES
                     APAWSPDGQRLVFVGYVGDGVDLFTINADGTELRNLTNIAKHTIQPNWSPDGEQIVYV
                     RSDADSSDIHVMNADGSASRPLTSGLGDESNPVWSPNGEWIAFLRTVFVPATNDADRA
                     ATEAATDEHSTADSQAAEGEFQTTICLMQPDGSDQRVLATVQFAEGLQWSPDGTQLAF
                     STFSSFRENQHAQVVGVDGSDQRSLDNGAVESSLPRWSPDGARISVVVIDPGGSAGSL
                     VVMNADGSDAHSIAELAGWNAWSPDGSQILVSQGRITRDGQSTGRSNLYLVNADGTDP
                     GQVLLLDDAAIDGDPAWHPGSQP"
     gene            4555..4965
                     /gene="mscL"
                     /locus_tag="VFV93_10985"
     CDS             4555..4965
                     /gene="mscL"
                     /locus_tag="VFV93_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX5165910.1"
                     /translation="MTAIWNEFKAFAFKGNLIDLAIAFVLGVAFTAVITSLVNDIIMP
                     IVGGIVSDKSFAGLTFEFLGVQVFYGNFLTALVYFLIVAWVLFMIIKAINRMQRPAAE
                     EAPATRECPYCLSSIPRLARRCPNCTSEVAPATA"
     gene            5063..5935
                     /locus_tag="VFV93_10990"
     CDS             5063..5935
                     /locus_tag="VFV93_10990"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5165911.1"
                     /translation="METRRLGRLGHMSSVIIYGGAALGAVSQDVADASIQLALENGIN
                     HFDTAASYGDSELRLGPWMPRIRNQIFLATKTTKRTRDEALREIERSLERLQVQSVDL
                     LQLHSIGEIEQLDLATGPGGALEGAIAAQEQGLIGAIGITGHGHKAPAVHREALRRYP
                     FATVLTPLNPVLYRDPGYRRDFDALVTEVRAQDAGLMTIKTVARRPWPQDAAHTHATW
                     YEPLVDQAQIDAAVAFVLSHEAITGLATAGDVHLLPLIIQAEARRREVTPAVIEAALP
                     VSAEYVSPFAEGGP"
     gene            complement(5952..6182)
                     /locus_tag="VFV93_10995"
     CDS             complement(5952..6182)
                     /locus_tag="VFV93_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5165912.1"
                     /translation="MTVHDDHYHINVFWSDEDESFVADIPDFQYCSAFGATPEEALRE
                     VLIAKRLWLESAREHGRPIPEPRYRPLIYQIA"
     gene            6181..6387
                     /locus_tag="VFV93_11000"
     CDS             6181..6387
                     /locus_tag="VFV93_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165913.1"
                     /translation="MLQSQSVALNQSEKLSNLIRLGFAPQWLEIHLRWDGRVNVDVMA
                     TFRSIERKAEAFDEIDEISKRFTL"
     gene            complement(6421..7128)
                     /locus_tag="VFV93_11005"
     CDS             complement(6421..7128)
                     /locus_tag="VFV93_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX5165914.1"
                     /translation="MTVQERVSMTEKQLAAICNRYHVRELALYGSVLREDFRDDSDID
                     VLVEFEPDASIGFSDLIGLQQDLADTVGREVDLVERRSLHPANRDAVLEQAQTIWPEW
                     QALDIMPARPFRREWLQLHVMIEAADDLAEMLDGQNRATVIGSDLRRSALIVPLTRIG
                     HLAGRLPDDIRRRYSDVDWHGWEERGDRLTRRYWDIDWNMVWTMAVDEAPRLREYIAR
                     ILESEFSDSAASQHNES"
     gene            complement(7179..7826)
                     /locus_tag="VFV93_11010"
     CDS             complement(7179..7826)
                     /locus_tag="VFV93_11010"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX5165915.1"
                     /translation="MAEIPVRVRKLTRKSPDQQSTSGVDHDPGDDVDNYEEVSLISSS
                     DHLLESEQPIFTSTDPEQWNEFLLEALLSIAPATFERLCQRVLRESGFTKVEVTGRSG
                     DGGIDGIGVLRVSLLSFHVYFQCKRYRGSVGASAIRDFRGAMVGRTDKGLFITTGTFT
                     PDAKKEATRDGAPVLDLIDGTALCALLKDLKLGITTEMVEAVTVDSHWFQSLASR"
     gene            8486..>10693
                     /gene="acnA"
                     /locus_tag="VFV93_11015"
     CDS             8486..>10693
                     /gene="acnA"
                     /locus_tag="VFV93_11015"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX5165916.1"
                     /translation="MSTTSDPFGARATLHYSGGEVTWFRLAALADYGDIRRMPVTVKI
                     LLENVLRNVSLGEDGFDPAAVETLARWTPNGSGEGELPFLPARVLLQDFTGVPAVVDL
                     AAMRSAMQRLGGDPARVNPLLPADLVIDHSVAVDTFGTMLAYGSNVEMEYQRNRERYA
                     LLRWAQHAFDGMSIVPPGTGICHQVNLEFLSKVVQAREVRGEMVAFPDTCVGTDSHTP
                     MVNGLGVLAWGVGGIEAEGAMLGQPIYMLPPKVVGVRLVGELPEGATATDLVLTVTQM
                     LRKHGVVGRFVEYCGPGLSKLPVADRATIGNMSPEYGATCGLFPVDAETLRYMRMTGR
                     PDDVVDLVERYTKEQGIFRTDETPDPQFDELLELDLGTVEPSMAGPRRPQDRVSLAGV
                     SQALRDAFGEQMAPPKQPGETLAEATQAAAVADTFPASDPMPAQEEKPGGSVESAEEL
                     CERVLPTAEELVQESTREGATIKLTPMTADNTVSADDGTTADIVLGPDEIATIGHGSV
                     VIAAITSCTNTSNPSVMLAAGLLAKKAVERGLRTKPYVKTSLAPGSRAVADYYDNAGL
                     MPYLEALGFHIVGYGCTTCIGNSGPLIPAVGQAVDEDNLVVAAVLSGNRNFEGRIHSQ
                     VRASFLASPPLVIAYALAGTVDVDLTTQPIGNDPDGQPVYLRDIWPSLEEVRDVVTSA
                     VTADVFSTSYASVFEGDETWRSLEVPTGQLYAWERESTYIQEPPYFENFQMEPP"
BASE COUNT         2035 a         3456 c         3294 g         1908 t
ORIGIN      
        1 agccggctgc ctcaagcgct tcccgcgcga tcagcgccgt ccgcaggttg acctgatact
       61 cgatccccag cgcgctcggg aacatccggt catggcccgg atcgaggacg atgaccggcg
      121 tcgcgtcgcc ggcgcctgac ggggtcggtg acatcgtggc tgtcggatca accggcgttg
      181 ccgtgctggt cgatgcgggt aaaggcgtta ctgtcgcagt tgccgtcagg ccggcagccg
      241 gatcggtcgg tgtcgaggtg gcagtactgg gtatgctcgt cgcgatgcca ggcgtgactg
      301 tcagccgaat cggcggtggt tcggtcaaga cggtcgcgag cagcccatcc ggctccgaca
      361 cacctgccga gccaatgcag ccggccagaa gcagggcgag gagcagcaac acaattgaaa
      421 aacgatgagg ttgtggtgtg cgttgcatca gtcccatgct gcccccgcga tacgcgtgaa
      481 cgggcacatc gtggcatcct ggtcgccagc ggatggccgc tatgggccag cgtcgaggat
      541 cttgaggaac atggaaccgg tgacgatcgg gatatcctcg tatgaatcgc gatcaagcag
      601 atctcgatct tctgaaacga gcaggtctac gttcccaacc gtagcggttg ccagaaaggg
      661 atcgtccttt ggatcgcgcg aaacagctgg gacgtcctcg tactccacga cctcggcctg
      721 ggcgagccac gtgagaacag catgcggatc ccgagtgtca acactccggt acttgcgaac
      781 cagttccgga cgcctgatga cctccagata ctcggcaacg atcgacggag agaccacctg
      841 ctggtagtca ttcgcacgat cgaagacgat gcgcccccat tgaccatacg ggttgatgag
      901 tccacgaacg atgatcaccg tgtcgaagac gactctcttc accggctgga gcgcctcgat
      961 gcccgcgccg ccttgatcgc agcgtcaatg tccgcgttca cctcttcctc agggatgttg
     1021 cgctcgatga tctcctgccg gacgggcgcg aagtaggcat agagttcccg aagccatggt
     1081 gaaccctcgg ttgcgtcgct cagacggacg ggcctgatgc gaagttctcc gtcatcgagc
     1141 cgtacctgga gcatcgtttc gtccgtgatc cccagccgct tgcgaaactc ggccgggatg
     1201 gtgatctggc ctccgcgtaa cggccgaacg agcttggtca tcgttgtcga ctcgctcatt
     1261 gcagataccg tccactctcg ccgagcctga tactgttcac acatcaacag attctgcatt
     1321 ttacaatatg tcaccatctt tcgacaggaa gagtgctaac ctcaactcgc gtcgagccga
     1381 gagtcgtagc aaaatgacgc cagcggccgg gcgtatgctt cttgcggcgc tgcgggcgga
     1441 atccccacga gccggctcga tgacgggcgc ggcaggttcg acaccgtcgt cgatcagcgc
     1501 ggaaaggcgg agcaggatgg ccagcgatcc tgaggtcacg acaggtacgg gcggtttgtt
     1561 cgagctggac gatgtccacc gtgagttcca gcaggtctgc cgcgacttcg tcaacgagca
     1621 gatcatgccg aaggtcgagc gggccgagcg cgaggggcag ttcccgctgg agctgatgcc
     1681 gctgatgggg cgctacggct tcctcggcct cggcctgccg gaagatgccg gcgggaccgg
     1741 cggcgacatg ttggcgatcg cgatcctgtc cgaggagttg ggccgatcct ccggcgggat
     1801 cgccgttacg ccgctggcct ctgcctacat ggccgcgccg catctggcga agtacggcac
     1861 gcccgatcag cagcaacgct acctgctgcc gatcacgacg ggcgagaaga tcgcctcgat
     1921 cggcgtcacc gagccgggag caggctccga cgtggccggg atgcgaacga cggccaggga
     1981 gacgaccggc ggctacctga tcaacggcac gaagctgttc atcaccaacg gcggcttcgc
     2041 cgactacatc gtcctcggtg cgaagaccga cccgaccgag cggcaccgcg gcatcacgat
     2101 gttcctgatc gagaaggacg accccggctt ctcgctcggc cgtccgttgc agaagatggg
     2161 ctggcactcc tctgataccc gtgagctgat cttcgaggac tgcttcatcc cggccgaccg
     2221 cgtcattggc gaggaaggac gcggcttcta tcagatcgcg tcgtccttcc agaccgaacg
     2281 gattacgctc agcgggatgg gcgtcggcct ggctcaggcc gccttcgacg acgcgctgca
     2341 atacgccaag gaccgcgagg cgttcgggca ggcgatcggt aagtaccagg cgatccgcca
     2401 catgctggcc gcgatggcga ccgatatcca gacgggcagg ctgctgctct accaggccgc
     2461 cgccaagctc gacagcaacg cgcccgacgc gctggatgtc gtcgcgatgg ccaagtacca
     2521 cacagccatc atcgccaaca cggtcgccga tcgggccgtc cagatcttcg gcgggatggg
     2581 ctacatcgaa gagaccaggg tcgcgatgca ctaccgcgac gcccgcatcc tccgcatcgg
     2641 cggcggcacc gacgagatcc agctggagat cctcgccaag cggatggggc tgtagccgtc
     2701 agtgatgagc gagcagcctc ctggtgtggt ttgacacgat ccacacgtga ccagcaagcc
     2761 tgatggatga cggctacacc aacggcaccg tccatcgggt ggccgtcaaa gccggcagag
     2821 cgtctgttcg acatcgcgca ccgccgtctg gattgatcga actccaccgg catcttgcta
     2881 tcgatcatcg atctcggcac ctcggaactc gcgtcctggt gcggcagcga tgatggatgg
     2941 aaccgagatg gctgggacaa cgtctctacg ctcagggatg tcatcagaat cgacagtggt
     3001 gtgcgtcgcg atagggcagg agcgttggcg tgacgaactt cgcacgaatc cacggcgcga
     3061 ccgctctgct ctgtttcctc gttgcaagcc tgcttgcggg ctgtggcgaa ccagctgaca
     3121 tctctgagcc ggccaaacag ccacggaccg cagcgtcgcc cgacacgacc accgcgtcac
     3181 cgacgaggac cagcgtacca acacctccga tcacccccgg cagcaccgac gtgcctgacg
     3241 atgcacgaga catgccgagt tggcccatcg tcgtcgctct cgatcccgat ggtggcgagc
     3301 agtacaagct gcacctgatc gagacggacg gggcactgcg gcggctcaca ccgcaagact
     3361 cggagatcat cggcgccgag tcggccccgg cctggtcgcc ggacgggcag cgcctggtct
     3421 ttgttggcta cgtcggtgat ggcgtcgatc tcttcacgat caatgccgac ggaaccgagc
     3481 ttcgcaacct gaccaacatt gccaagcaca cgatccagcc caactggtca cccgacggcg
     3541 agcagatcgt ctatgtccgt tcggacgccg acagcagcga catccatgtc atgaacgcgg
     3601 acggatctgc gagccgcccg ttgacgagcg ggttggggga tgaatccaac ccagtctggt
     3661 cgccgaacgg tgagtggatc gcgttcctcc gaaccgtctt cgtcccagcc accaacgacg
     3721 ctgaccgtgc tgcgaccgaa gcggcgacag atgaacactc cactgctgac agccaggctg
     3781 ccgagggaga atttcagacg acgatctgcc tgatgcagcc cgacggcagc gatcagcgcg
     3841 tgctcgcgac cgtccagttc gccgaggggt tgcaatggtc gccggatgga actcagctgg
     3901 cgttcagcac cttcagcagc ttccgcgaaa accagcacgc ccaggttgtt ggtgtggatg
     3961 gctccgatca gcggtcgctt gacaatggcg ccgtcgaatc gtcactgccc cgctggtcgc
     4021 ccgacggggc gaggatcagc gttgtcgtga tcgatccggg cggttccgcg gggtcgctgg
     4081 ttgtcatgaa cgccgacggc tcggacgcgc attccatcgc ggagttggcc ggctggaacg
     4141 cctggtcacc ggacggcagc cagatcctcg tctcgcaagg acggatcacg cgtgatggtc
     4201 aatcgactgg ccgctcgaat ctgtacctcg tcaacgccga tggcaccgat ccggggcagg
     4261 tcctgctgct ggacgacgca gcgatcgacg gcgatcccgc ctggcatcca ggatctcagc
     4321 cgtaaacacc gaaggcgtta acagacggcc catgtacgca ggttgtgtgc tatcgtaggc
     4381 caacgccgtc tggccagatg atgctcgatc gttgcagaga aaggggtcgt actcacgggg
     4441 acgcccgtgc tgtcgtcaca gccgggaccg gtccagcaac tgacggacgc tggccactcg
     4501 tggggatgac ggccagcctg cattgacatg gatggatgga agaggcggaa ccgtatgact
     4561 gcgatctgga atgaattcaa ggcgttcgcg ttcaaaggga acctgatcga cctggcgatc
     4621 gctttcgtcc tcggcgtcgc cttcacggcc gtgatcacat cgctggtcaa cgacatcatc
     4681 atgccgatcg tcggcggaat cgtcagcgac aagagcttcg ccggactgac gttcgaattc
     4741 ctgggagtgc aagtcttcta tgggaacttc ctgacggcgt tggtctactt cctgatcgtc
     4801 gcctgggtgc tcttcatgat catcaaggcc atcaaccgca tgcagcggcc agccgccgaa
     4861 gaggcgcccg cgacccgcga gtgcccgtac tgcctctcgt cgatcccgcg cctcgcccgc
     4921 cgctgcccga actgcacctc ggaggtcgcg cctgcaacgg cgtgaacgaa tggatgggga
     4981 tggggcatgg ggatgagacg aggattcgtc tcgaatctca tcccccatgc ctcatccctc
     5041 atccccgaat cggagggttc tgatggaaac acgacgcctc ggacgcctcg gccacatgag
     5101 ttcggtcatt atctacggcg gagcagcgct cggcgcggtc agccaggacg tcgccgacgc
     5161 gtcgatccag cttgcgctgg agaacggcat caaccacttc gacacggccg ccagctacgg
     5221 cgactccgaa ctacggctcg gcccgtggat gccgaggatc cgcaaccaga tcttcctggc
     5281 aaccaagacg accaaacgga cacgcgacga ggcgttgcgc gagatcgagc gctcgctcga
     5341 acggctccag gtccagtcgg tggatctgct gcaactgcat tcgatcggtg agatcgagca
     5401 actcgacctg gcgaccggcc ccggcggcgc gctggagggc gcgatcgccg cccaggagca
     5461 ggggctgatt ggagcgatcg gcatcaccgg ccacggccac aaagcgcccg ccgtccaccg
     5521 cgaggcgcta cggcgttacc cgttcgcgac tgtcctgacg ccactcaacc cggtcctcta
     5581 ccgcgatccc ggctaccgcc gcgacttcga cgcgctggtg accgaggttc gcgcacagga
     5641 cgccggcctg atgacaatca agaccgtcgc tcgccgtccg tggccacagg acgccgccca
     5701 cacccacgcc acctggtacg aaccgctcgt cgatcaggcc cagatcgacg ccgccgtcgc
     5761 attcgtcctc agccacgaag ccatcaccgg cctcgccacc gccggcgatg tccacctgct
     5821 tccactcatc atccaggccg aagcccgccg ccgcgaagtg acgccggcgg tgattgaggc
     5881 cgcgttgcct gtgtcggcgg agtacgtctc gccattcgcc gagggtggtc cgtagtcgat
     5941 actggcatca atcaggcgat ctgatagatc aaagggcgat agcgaggctc cgggattgga
     6001 cggccgtgct cacgagcgga ctccaaccag agccgcttcg caatcaacac ctctcgtaat
     6061 gcttcctcgg gtgtcgcccc gaaagcagag caatactgaa agtccggaat gtcggcgaca
     6121 aaagactcat cctcgtcgct ccagaacacg ttgatgtggt agtgatcgtc atgcacggtc
     6181 atgctccaat cgcaatccgt agcgctcaac cagtcggaga aattgtcgaa tctgatacgg
     6241 cttggcttcg ccccgcagtg gttggaaatt cacctgcgtt gggacggcag ggtgaatgta
     6301 gatgtgatgg ctaccttccg ttcgatcgag cgtaaagccg aagccttcga cgagatcgac
     6361 gaaatctcta aacggttcac gttgtgaacc gatccctgcg tgagtcgacg gagcaggcgt
     6421 tcaggattca ttatgctggc tcgccgccga atccgaaaac tcggattcga ggattctggc
     6481 aatgtactcg cgcaatcggg gcgcttcgtc tacggccatc gtccatacca tgttccaatc
     6541 gatatcccag tatcgtcttg tcagacgatc tccacgttct tcccagccgt gccagtccac
     6601 atcggaatac ctgcgccgga tgtcatccgg aagcctgcca gccagatgac cgatccgggt
     6661 cagcgggacg atcaacgcgc tgcgccgtaa gtcactcccg atgaccgttg cgcgattctg
     6721 accatccagc atctcagcga ggtcgtcagc ggcttcgatc atgacatgga gttgcagcca
     6781 ctcgcggcga aatggacgtg caggcatgat atccaaagct tgccactcgg gccagatcgt
     6841 ctgagcttgc tccagtaccg catcgcgatt tgctgggtgt agcgagcgcc gttcaacgag
     6901 atcgacctcg cgaccgacgg tgtccgcaag atcctgttgt agcccaatga gatcggagaa
     6961 gccgattgac gcatcaggct cgaactcgac cagcacatcg atatcgctgt catcgcggaa
     7021 gtcctcgcgc agaaccgatc cgtagagcgc cagttcccgg acatgatagc gattgcagat
     7081 cgcggctagt tgcttctccg tcatggagac gcgttcctgg acggtcatca cagcttcctg
     7141 ataccgaaaa tctggattcg ccacacgcaa gattggtgtc acctcgacgc taaggactgg
     7201 aaccaatggg aatcaaccgt caccgcctca accatctcgg ttgtaatacc aagtttcaag
     7261 tccttgagaa gcgcgcacag ggccgtaccg tcaatgaggt caagtaccgg agcaccgtcg
     7321 cgcgttgctt ctttcttggc atccggtgta aacgtgccgg ttgtgatgaa caaaccctta
     7381 tcagtacgac caaccatcgc gcctcgaaag tcgcggatcg cggaggcacc gacgctgcca
     7441 cggtatcgtt tgcattgaaa atagacgtgg aacgagagaa gcgagacccg caacacgcca
     7501 atgccgtcga tgcctccatc gcctgatctg cccgtcacct caactttcgt gaatcccgat
     7561 tcgcgaagaa ctcgttgaca aagtctctca aatgtggctg gagcgatcga gagaagcgcc
     7621 tctaagagaa actcgttcca ctgttctgga tcggtacttg tgaagatggg ttgttcggat
     7681 tcaagaagat ggtcggacga tgagatcaga cttacttcct catagttgtc aacgtcgtcg
     7741 cctgggtcgt ggtcgactcc ggatgtcgac tgctgatctg gactctttct ggtcagtttc
     7801 ctgactctta caggaatctc ggccatgtcg tcgatatcga gttcgcggcc agcctcggtg
     7861 atcgctcaca cccctcgatt cgtgttggta accgcaccaa ccttcttaag atacgtacgc
     7921 gcccatgcta accggtattg gacctccgtc tgtggcccat cgccatgagg ttcctgcagt
     7981 tgctcgtcag acaaaccaga aaattcgaca accttatctt caatctctgc gatcgatgct
     8041 gagtctccga gcaacttgag cgcttggagg gtcggccaaa gcagcttgtc gtacgccggt
     8101 acgatcgact gacttgtctt gcctctgagc ataccgtctg tcctcatttc cttgtcaacg
     8161 atctggcgaa cccaccatca gcgaatctct ttgaacagca catcccctgt cgggaacgaa
     8221 tccctctgcg gacgattgac atcgcctacc ggctcaacta tgtgctcccc aggatacccg
     8281 acatacctcg accggcttca tcttcgacac acctgcggta ccatcagggc aggacagacg
     8341 cgcgggccgg tggcgatccg gcacgcgcga tggacggcca caccgcaggg acgatccagg
     8401 gaccgccaga catgccctga ctcacgtcca cccgccggcg ctggttctgg ttgaggggtt
     8461 cgagctacga gggaggcttc agcagatgag cacaacgtct gatccgtttg gcgcacgcgc
     8521 cacgcttcat tattcaggcg gcgaggtgac ctggttccgc ctggcggcgc tggccgatta
     8581 cggcgatatt cgccggatgc cggtcaccgt caagatcctg ctggagaacg tcctccgcaa
     8641 cgtcagtctc ggcgaggatg ggtttgaccc ggcggcagtc gagacgctgg cgcgctggac
     8701 cccgaacggc agcggcgagg gcgaactgcc cttcctgccg gcccgggtgc tgttgcagga
     8761 cttcaccggc gtcccggcgg ttgtcgatct ggccgcgatg cgttcggcca tgcagcggct
     8821 gggtggcgac ccggccaggg tcaacccgct cctgccggcc gatctcgtca tcgaccactc
     8881 cgtcgctgtc gacacgttcg ggacgatgct ggcctatggc agcaacgtcg agatggagta
     8941 ccagcgcaac cgcgagcgct acgcgctgct gcgctgggcc cagcacgcgt tcgacggcat
     9001 gagcatcgtc ccgcccggca ccggcatctg ccaccaggtc aacctcgagt tcctctcgaa
     9061 ggtcgtccag gcgcgcgagg tgcgcggcga gatggtcgcc ttccccgaca cctgcgttgg
     9121 gaccgactcg cacacgccga tggtcaacgg tctcggcgtg ctggcctggg gcgtcggcgg
     9181 gatcgaggcc gagggcgcga tgctgggcca gccgatctac atgctgccgc cgaaggtcgt
     9241 cggcgtcagg ctggtcggcg agctgccgga gggcgcgacg gcgaccgacc tggttctgac
     9301 cgtgacccag atgctgcgca agcacggcgt cgtcggccgc ttcgtcgagt attgcgggcc
     9361 gggcctgagc aagctgccgg tcgccgaccg ggctacgatc ggcaacatgt cgccggagta
     9421 cggcgcgacc tgcggcctct tcccggtcga cgccgagacg ctgcgctaca tgcggatgac
     9481 cggccggccg gacgatgtcg tcgatctggt cgagcgttac accaaggagc agggcatctt
     9541 ccgcaccgac gagacgcccg acccgcagtt cgacgagctg ctggagctgg acctcggcac
     9601 ggtcgagccg agcatggccg gcccgcgccg cccgcaggac cgcgtctcgc tggccggcgt
     9661 cagccaggcg ctgcgtgacg ccttcggcga gcagatggcg ccgcccaagc agccgggcga
     9721 gacgctggca gaggcaaccc aggcagctgc tgtcgccgac acgttcccgg cctccgaccc
     9781 catgccggcc caggaagaga agccgggcgg cagcgtcgag agcgccgagg agctgtgcga
     9841 gcgggtgctg ccaacggccg aggagctggt ccaggaatcc acccgcgagg gcgcgacgat
     9901 caagctgacg ccgatgacgg ccgacaacac cgtctcggcc gacgacggca cgacagcgga
     9961 catcgtcctc ggcccggacg agatcgcgac gatcggccac ggctcggtcg tcatcgccgc
    10021 catcacgagc tgcaccaaca cctcgaaccc atcggtcatg ctggcggccg gactgctggc
    10081 caagaaagcc gttgagcggg ggctgcgaac caagccgtac gtcaagacca gcctcgcgcc
    10141 ggggtcccgc gccgtcgccg attactacga caacgccggc ctgatgccgt atctcgaagc
    10201 gctcggcttc catatcgtcg gctacggctg cacgacctgc atcggcaact ccggcccgct
    10261 gatcccggcc gttgggcagg ccgttgacga ggacaacctg gtcgtcgcgg cggtgctctc
    10321 cggcaatcgc aacttcgagg gccgcatcca ctcccaggtg cgcgccagct tcctggcctc
    10381 gccgccgctg gtgatcgcct acgcgctggc cgggacggtc gatgtcgacc tgacaaccca
    10441 gccgatcggc aacgaccccg acggccagcc ggtctacctg cgcgacatct ggccgagcct
    10501 cgaagaggtt cgcgacgtgg tgacctcggc cgtcaccgct gacgtcttct cgacctccta
    10561 cgccagcgtc ttcgagggcg acgagacctg gcgctcgctg gaggtcccga ccggccagct
    10621 gtatgcctgg gagcgggaat cgacctacat ccaggagccg ccctacttcg agaacttcca
    10681 gatggagccg ccg
//
LOCUS       DASUGJ010000492        11809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1606555, whole genome shotgun sequence.
ACCESSION   DASUGJ010000492 DASUGJ010000000
VERSION     DASUGJ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11809
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606555"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..567
                     /locus_tag="VFV93_11020"
     CDS             334..567
                     /locus_tag="VFV93_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX5165917.1"
                     /translation="MTSTHDKRNRVRLSLEVPQELNALLEELAAETDSTKSEVLRKAI
                     ALMDVAVDAKREGKRVFVSDQVPAGASREIVGI"
     gene            564..875
                     /locus_tag="VFV93_11025"
     CDS             564..875
                     /locus_tag="VFV93_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165918.1"
                     /translation="MSSPSPFDLERIVREGGLEVEIRSSPTPADAAHRRQLEDDEARH
                     LRWMTKAIFVVLLAIAIIKLFIILLADDAATKNWARNLFAGIVGLLAGFAGGSRLSSA
                     R"
     gene            976..1719
                     /locus_tag="VFV93_11030"
     CDS             976..1719
                     /locus_tag="VFV93_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX5165919.1"
                     /translation="MSSSSAAASSQASESTSDVDSFSRVMIVVAHPDDAEFSSAGTLA
                     KFAREGKHVVLVQCTSGDKGTARRDISSPELAKLREGEQLEASRRTGIAETVFLRLGD
                     GELTPDLAFREKVVRQIRIHRPDILITHDPFRPYALHPDHRAVGITAVDSVYPTARDP
                     LYFPQHLQEGLEPHKVAELWLYGSEAPDTFIDISETFETKIHALKAHQSQVGTAETLE
                     QRMRDRAEELGRQHGLPMAEAFKVVKMRR"
     gene            1759..2952
                     /locus_tag="VFV93_11035"
     CDS             1759..2952
                     /locus_tag="VFV93_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5165920.1"
                     /translation="MEENIIREKLAQAVQVLDEEDVDLWMLVGRETDTLGDPSLPLVV
                     GTSVTWESAFLVSRTGQHVAIVGTGDVENIKQTGAWDNVVGYVEGISAPLVEALDRLN
                     PNVIALNYSTDNNMADGITHGMYLLLEEQLRGTPYWERVRSAGEIPTKVRARKSPTEL
                     KRIREAVRTTERIWDAVGGWLRPGLTEREISTFMHDQLEERRIGSSWDWKYCPTVTAG
                     PDSPRGHVGPTDVQTQAGHLITIDFGVNQEEYSSDMQRTFYFLKPGETEAPAEVTRHF
                     AIVDRCIQEAAAFIKPGVLGWQVDDIARQIFAEADLEEWQFALGHQMGRACHDGGTVI
                     GPRWERYGQRPYGALEENEVYTLEIGCLVPGYGYVSQEEDIVVTSDGCEFLAPPQREV
                     MLLRS"
     gene            3028..3705
                     /locus_tag="VFV93_11040"
     CDS             3028..3705
                     /locus_tag="VFV93_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165921.1"
                     /translation="MAEKLKVTLELGPKGKRVVAVAPGWPGLSRGAKTEDAAVERLLE
                     YLPRYEPVAQLAGLGPAFPSATDVEIVEQYPGTGSTDFWGISFAFSEHDRQEMSRETL
                     ERELALMRASWSFFDGVRARVSAEMQKGPRGGGRDRDRIVRHTLVAELDMSKKVGVRT
                     PPEVILTEEGLLEHRDTYCDAIRAYHTEGKTPRTWPLRYVIRHTAFHTLDHAWEMEDK
                     DLTGKEV"
     gene            3833..4858
                     /locus_tag="VFV93_11045"
     CDS             3833..4858
                     /locus_tag="VFV93_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX5165922.1"
                     /translation="MSKSTPGRALRTLPGAGRLSRSGGARRRPTIETPSVETHYRLVE
                     HAGMRWYDIERPTSADLDYLRDEFAVHPLLIDDIISRSQRPKLDIYDDYLFVVMHFPV
                     HMKQTRTTIASEVDIIVGPDYVVTSHDGNLKPLMRVFESAIESEETRQALMSQTTAHL
                     LYYVIDRLVDYCFPVVGRLTDRIEEIEDAIFSRSDLRLVQEISIVRRDLIAMRRIIKP
                     QMPVISQLEHRAIPLIHSDIDVYFGDIADHLAKLWDSLEDLKEVLEGLSDTFDSLATH
                     RLNGVIKTLTVISVLVLPLTFITGLFGMNVPIPYENSSFALLIICAFLLLITVVMLAL
                     FRRRGWI"
     gene            4849..6354
                     /locus_tag="VFV93_11050"
     CDS             4849..6354
                     /locus_tag="VFV93_11050"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5165923.1"
                     /translation="MDLSLREAPAWQLVLLAAVGAIAAGGLVGRLLVTRGAVQTLALL
                     LAIGVGAALLRSVWVAFAGVVAVAGGLPFATLPVGASVTPTLLEAALLVALVSGGAVL
                     LIDRRQQIRTGGPQALALGLAGVVVAAFLLGLGRGYTTQTLHDFFKFLLAMATFWMVV
                     QLVIDTRAARGLLWLLVAATSGAAALGLALYAAGPGLSERVLVRLVPYGYPGSRIVRY
                     IEDDPARPMRAVGTSVDPNSFGGLLMIGFVLAAGQLLVRNRTIPAWLAGSAAAVCGLA
                     MLLTYSRGAWVGAFAGLVVIVVLRRPWLLLPIGGLGVAAVALGLGAGFIERLWLGFTL
                     QDPATKLRLDEYRNALAIIREHPWFGVGFGDAGSIELQAGVSSIYLTIAERAGLVGLA
                     CFMVVAGVIAWRGLRSSWKDDRSEDTDLTLCLTAAFVAALVVGLVDHYFFNPQFPHMA
                     AIFWILAGGIAAIASPVGYARARHRVSSDITQERWTSRRRLRQGLATRNGG"
     gene            6357..7259
                     /locus_tag="VFV93_11055"
     CDS             6357..7259
                     /locus_tag="VFV93_11055"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5165924.1"
                     /translation="MAGRQIEGIFAPVATLFDSDGEMDLAKYRKNMEWYAGSPLDGVV
                     IMGSNGEYASLDTDEKLRLIEAGTEAIGGRKKVLVGTGVESTRGTIRLTRAAAEMGAD
                     YALVVTPYYYRPRYDKAAFLQHYQAVAEASPIPVLIYVMTAYTGVDLPSAVVAELSRH
                     PNIVGVKDSAGNAVKFSEMVSDSDDDFSVLAGSANFLYPALCLGAAGGILALADIAPA
                     ACVEVRDLFAAGDHERAREAQFNLLAPNAAVTTRFGIAGLKSALAMVGLETSDPRPPM
                     LPSTDEERAQIRAILERAGILARV"
     gene            7246..7770
                     /locus_tag="VFV93_11060"
     CDS             7246..7770
                     /locus_tag="VFV93_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5165925.1"
                     /translation="MPEFEWLAHPIRIGLVADTHRRNPKRGLPPALLDGLTGCDMIFH
                     AGDISERWVLDLLEEIAPVRAVIGNNDSFELLKTLPEERYFVSGSHRIGLLHGYSDMA
                     KKATTAKVWTLGRMRGVVDCAVYGHSHQPEIAEREGLLMINPGSPTDFRWAKGPTYGI
                     LEVGEAIRARLIEL"
     gene            7973..9685
                     /gene="rpsA"
                     /locus_tag="VFV93_11065"
     CDS             7973..9685
                     /gene="rpsA"
                     /locus_tag="VFV93_11065"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX5165926.1"
                     /translation="MDRSDFDEAATRLQGSVGFKDDEAADEVGRSLMEQLLADPSHDY
                     RTLKYGDVVDGTVMHVDRDEILVDIGSKSEGVIPAREFSTLDDQERKSMGVGDQLLVF
                     VVQPENQDGHAVVSIDRARQEKSWRILQEQYEAGQVISAEVVNYNKGGLLVNLDGVRG
                     FVPASQVTEIRGGDDAQKQADMARLIGNSLQLKIIEINRHRNRLILSERQALQERRDE
                     MKEKLIEQLREGEVRQGRVSSICDFGAFVDVGGADGLVHLSELSWSRVRHPSEVLHVG
                     QEVDVFVLGINAEEKKIALSIKRTQAEPWSRVAAKYDVNQLVIGTVTQLANFGAFARI
                     EDGIEGLVHVSELSDARISHPRQLVSEGQELLLRIIRIDPQRRRMGLSLRRALEASDD
                     EVREALGDEAVELKQRLMALEVEPDPDAELEPERETSRSSAIAEPEVVVEPEGPATAV
                     EQVEVSNAMTEAFAAAEAAAAATNGTEDDAQVAVSDVADGDEPDVAADAAEAEVETPV
                     AETEEVAAAGEPVAESEAATVAESAAEQAPEEVAASASTETDETGAVSAAPASDDESE
                     KRMS"
     gene            9932..>11809
                     /locus_tag="VFV93_11070"
     CDS             9932..>11809
                     /locus_tag="VFV93_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX5165927.1"
                     /translation="MADKFEKFTERARKVLTLAQEEAQRFNHNYIGTEHLLLGLVREG
                     DGVAARVLSNMGVQLPKVRSAVEFIIGRGESVIMGEIGLTPRAKKVIELAVDEARRLN
                     HHYIGTEHLLLGLVREGEGIAAGVLESLGVNLEKVRAQVMQVVSQNAGYQQSKQATKT
                     PYMDALGFDLTEAARQNKLDPIIGRQNEIERVMQILSRKTKNNPALIGEPGVGKTAIV
                     EGLAQKIIAGDVPELLQNKRLVALDIGALVAGTKYRGEFEERLKKIVAEVKETGAILF
                     IDELHTLVGAGAAEGAVDAANILKPALSRGEVQTIGATTLDEYRKYIERDAALERRFQ
                     PIQVPEPNVEETIQILYGIRERYEEHHKLKITDESITAAANFAARYVTDRFMPDKAID
                     LIDEASSRVRMYRSASPPSLKEAMRGMESLQRELDAAVAGQEFELAADLRDRERKLRM
                     RIAELEKDWKEEQGSEMPEVSEEDIAQVVSMWTGIPLTRLAAEESERLLHMEAALHER
                     IVGQDEAISTMAKAVRRARAGLKDPRRPIGSFIFLGPTGVGKTLLARALAEFMFGSED
                     ALIKIDMSEFMERHTISRLVGAPPGYVGYEEGGQLTEAVRRKSYSVILLDEIEKAHPE
                     AF"
BASE COUNT         2180 a         3450 c         4038 g         2141 t
ORIGIN      
        1 tcgcagcggc gcgtcattcg cggacgaggt ttcgtctctc acgtccaggt attctacggg
       61 acgcggcaag tagtaacacc gttcgtcggc ccacccccat ggcatcgcca attctctttg
      121 tcatcctgag acgtgagccg atcggcccga cgcaggagcg acccttcggc tcacgtctca
      181 ggatgactaa gagaacgggt cctcgttcgt caacggacgt tttgaacatt tgaggaactg
      241 gcggagcgtg tgacgcagcc tgattgacgt acgagtgtag aagttacaca gaaaggtcgt
      301 aaatttcact tggacatagc tttggagctt ccgatgacct ccacccatga caagcgcaac
      361 cgtgttcgac tgagcctgga agttccccag gaactcaacg cgctactaga agagcttgcc
      421 gccgagaccg actcaaccaa gagcgaggta ctgcgcaagg ctatcgcgct gatggatgtc
      481 gccgtcgatg ccaagcgcga aggcaaacgc gtctttgtca gcgaccaagt accggctggc
      541 gcttcgcgag agatcgtcgg gatttgagca gcccgtcgcc gttcgatctc gagcgcattg
      601 tccgtgaggg cggcctcgag gttgagattc gcagttcccc gactcctgct gacgccgctc
      661 atcgtcgcca actcgaagac gacgaggcgc gccatctgcg ctggatgacg aaggccatct
      721 tcgtcgtgct tcttgcgatc gcgatcatca agctgttcat catcctcctg gccgatgatg
      781 cggcgaccaa aaactgggct cgcaacctgt tcgccgggat cgtgggtttg ctggctggat
      841 ttgcgggtgg ctcgaggttg agctcggcga gataacttga cgaactgggt gaggtcgtga
      901 atagtggccg gcgctgcccg gtaccgccag ccgagcgtac ggcgttgtca taactacgag
      961 ggggattgtt ccaccatgtc ttccagttca gcggctgcaa gcagccaggc atcggagtcg
     1021 accagcgatg tcgattcgtt ttcgcgggtg atgatcgtcg tcgcccaccc ggatgacgcg
     1081 gaattctcgt cggccggtac gctggcgaag ttcgccaggg agggcaagca cgttgtcctg
     1141 gtgcagtgca cctcgggcga caagggtacc gctcgccgag acatcagctc gcccgaactg
     1201 gcgaagctgc gcgaaggcga gcaactggaa gcgagcaggc gaaccggaat agcggagact
     1261 gtcttcctcc ggcttggcga cggtgagttg acgcccgatc tggcgttccg cgagaaggtc
     1321 gtccgccaga tccggatcca ccggccggac atcctgatca ctcacgaccc gttccggcca
     1381 tatgcgctgc atcccgacca ccgcgccgtc ggcatcaccg ccgtcgattc ggtctacccg
     1441 acggcccgcg acccactgta tttccctcag catcttcagg agggactgga gccgcacaag
     1501 gtcgccgaac tctggctgta cggctccgag gcgccggaca cgttcatcga catcagcgag
     1561 acgttcgaga caaagatcca cgcattgaag gcacatcaga gtcaggtcgg aacggccgag
     1621 acgctggagc agcgcatgcg tgaccgcgcc gaagagcttg gccggcagca tggcctgccg
     1681 atggcggagg cgttcaaggt cgttaagatg cgccgctaaa ggattgtcgg gacgtcggag
     1741 gcgaaggggc tggctgaaat ggaagaaaac atcattcgcg agaagctggc acaggcggtc
     1801 caggtcctgg atgaggaaga tgtcgatctc tggatgctgg ttggccgtga gacggacacg
     1861 ctcggtgatc cgagtttgcc actggtggtt ggcacgagcg tgacgtggga gtcagccttc
     1921 ctcgtctcgc gaaccggtca gcatgtcgcg atcgtcggga ccggcgatgt cgagaacatc
     1981 aagcagaccg gtgcctggga caacgtcgtc ggttacgtcg agggcatctc ggcgccgctg
     2041 gtcgaggcac tggacaggct gaatccgaac gtcatcgcgc tgaactactc gaccgacaac
     2101 aatatggcgg acggcatcac ccacgggatg tacctgctgc tggaagagca attgaggggg
     2161 acgccgtact gggagcgggt gcgctcggcg ggcgagatcc cgacgaaggt gcgggcgcgc
     2221 aagtcgccga cggagctgaa gcggatccgc gaagcggtcc ggacgacgga gcggatctgg
     2281 gatgcggtcg gaggctggct ccgtccaggg ttgaccgagc gtgagatcag caccttcatg
     2341 cacgaccagc tcgaagagcg acggattggc tcgtcgtggg actggaagta ctgcccgacc
     2401 gtcacggccg gaccggactc gccacgcggc catgtcgggc cgaccgatgt ccagacgcag
     2461 gccggacacc tgatcacgat cgatttcggc gtcaaccagg aggagtactc gtccgatatg
     2521 caacggacgt tttatttcct caagccgggc gagactgagg cgccggccga ggtgacccgt
     2581 cacttcgcaa tcgttgaccg gtgtatccag gaggctgcgg cgttcatcaa gccgggtgtg
     2641 ctcggctggc aggtcgatga catcgcacga cagatcttcg cggaggccga tctcgaagaa
     2701 tggcagttcg cgctcggcca tcagatgggc cgtgcctgcc atgacggcgg gactgtcatc
     2761 ggaccccgct gggaacgcta cggccagcga ccctacggcg cgctggaaga gaacgaggtc
     2821 tacacgctgg agatcggctg tctcgtgccg ggatacggct acgtcagcca ggaggaggac
     2881 atcgtcgtca ccagcgatgg ctgcgagttt ctcgcgccgc cgcagcgcga ggtgatgctg
     2941 cttcgatctt gacggttcgt ttatccggac aggtgaacgg cggctcgttc gacgtcgggc
     3001 atattttgct gtgtgctggg aggtgcggtg gcggaaaagc tcaaggtgac gctggaactt
     3061 gggccgaagg gcaagagggt ggtggctgtc gcgccgggat ggcccggact ctcgcgtggg
     3121 gcgaagaccg aggatgctgc ggtcgagcgg ttgcttgaat atctcccacg ctatgaaccg
     3181 gtcgcacagt tggccgggct gggaccggcg ttcccgtcgg cgacggacgt tgaaatcgtc
     3241 gagcagtatc ccggaactgg gtcaaccgac ttctggggta tctcttttgc gttttcggag
     3301 catgaccgac aggagatgtc gcgcgaaaca ctggagcgag agctggcgct gatgcgggcg
     3361 agctggagct tctttgacgg cgttcgggcg cgcgtgtcgg cggagatgca gaaaggcccg
     3421 cgcggtggcg ggcgtgaccg cgatcggatc gtccgccaca cgttggtcgc cgaactcgac
     3481 atgtcgaaga aagttggagt gcgtactccc cctgaggtga ttctgaccga ggagggtttg
     3541 ctggaacacc gcgacaccta ttgcgatgcg attcgggcgt accacaccga gggcaagacg
     3601 ccacgcacat ggccgctgcg ctatgtgatc cggcacacgg cgtttcatac gctcgaccac
     3661 gcatgggaga tggaagacaa agacctgacc ggcaaggagg tgtaagaacg tggaacgttg
     3721 caagtagcat cgccgcaatc aggtctgtgt gaagcgcctg tgctatcgtt tcgggcgaat
     3781 caggagatgc caacgatgtc aagcggctct gacgctggtg ggagtcaagc aggtgagcaa
     3841 gtcgactccg gggcgcgcac tgcgaaccct gccaggcgct gggcggctca gccgctcggg
     3901 aggggcgcgc aggcgaccaa cgattgagac gccgtcggta gagacgcatt accggctggt
     3961 tgagcatgcc ggaatgcgtt ggtacgatat cgagcgaccg acatctgccg acctggacta
     4021 cctccgcgac gagttcgccg tccacccgct gctcatcgac gacatcatca gccgcagcca
     4081 gcgtccgaag ctggatatct atgacgacta cctgttcgtc gtcatgcact tccccgttca
     4141 catgaagcag acccgaacga cgatcgcctc cgaggttgac atcatcgtcg gtccggacta
     4201 cgtcgtaaca tcgcatgacg gcaatctcaa gccgctgatg cgcgtcttcg agagcgcgat
     4261 cgagtctgag gagacgcggc aggcgctgat gagtcagacg acggcgcatc tgctctacta
     4321 cgtgatcgac cggctggtgg actactgctt cccggtcgtc ggcaggctca ccgacaggat
     4381 cgaggagatc gaagacgcga tcttctcgcg ctccgacctg cggctggttc aggagatctc
     4441 gatcgtacgg cgcgacctga tcgcgatgcg gcggatcatc aagccgcaaa tgccggtgat
     4501 ctctcagctg gagcaccgcg cgatcccgtt gatccacagt gatatcgatg tttactttgg
     4561 cgacatcgcc gaccatctgg ccaagctgtg ggactcgctc gaagacctca aggaagtgct
     4621 cgaagggttg agcgacacat tcgactcgct ggcgacccac aggctgaacg gggttatcaa
     4681 gacgctgacg gtcatttcgg tcctggtcct gccgttgacg ttcattaccg gactgttcgg
     4741 gatgaacgtg ccgattccct acgagaactc atccttcgcg ctgttgatca tctgcgcgtt
     4801 tttgctcttg attaccgtcg tgatgcttgc cctcttcagg cgtcgcggat ggatctgagc
     4861 ctacgcgagg ctccggcctg gcagctggtg ctgttggcgg ccgtcggtgc gatcgctgcc
     4921 ggcgggttgg tgggacggtt gctggtcacg cgcggcgccg tccagacgct ggccctgctg
     4981 ctcgcgatcg gcgttggcgc ggcgctactg cgctcggtct gggtcgcatt cgccggtgtg
     5041 gtcgcggtcg ccggcgggtt gccgttcgcg acgctgccgg tcggagccag cgtgacgccg
     5101 accttgctgg aggcggccct gctggttgcg ctcgtgtcgg gtggcgcggt cctcttgatc
     5161 gaccggcgtc aacagatccg gaccggcgga ccgcaggcac tggcgctcgg gctggcaggg
     5221 gtcgtcgtcg cggcctttct gctcgggctc ggccggggct acacgaccca gacgctgcac
     5281 gacttcttca agtttctgct ggcgatggcg acattctgga tggtcgtaca gcttgtgatc
     5341 gatacgcgtg ccgcccgcgg gttgctctgg ctgctggtgg cggcgacgag tggggcggcg
     5401 gcgctggggc tggcgttgta cgcggcgggt ccgggccttt ccgagcgagt gctggtccga
     5461 ctcgttccgt acggttatcc gggcagccgg atcgtgcgct acatcgagga tgatccggcc
     5521 cggccgatgc gggcagtcgg tacaagtgtt gatccgaact ccttcggcgg gctgttgatg
     5581 atcgggttcg tactggcggc ggggcaactg ctcgttcgga accggacgat acctgcctgg
     5641 ctggcgggtt cggcggccgc tgtttgcggg ttggcgatgc tactgaccta ctcgcgtgga
     5701 gcgtgggtcg gagcgttcgc tggtctcgtc gtcatcgtcg tcttgcggcg cccgtggttg
     5761 cttctgccga taggggggct gggagtcgct gccgtggcgc ttgggttggg ggccggcttt
     5821 atcgaacggc tctggctcgg gttcacgttg caggacccgg ccacgaagct gcggttggat
     5881 gagtaccgca acgcgctggc gatcattcgc gagcacccgt ggttcggggt cgggtttggc
     5941 gatgcgggat cgatcgagct ccaggctggt gtgtccagca tctacctgac gatcgccgag
     6001 cgggcaggtc tggtcggtct ggcgtgtttc atggtcgtgg ctggtgtgat cgcgtggcgc
     6061 gggttgcgca gttcgtggaa ggacgaccga agcgaggaca cagacctgac cctctgcctg
     6121 acggcagcat tcgtcgcggc tctggtcgtc gggctggtcg atcactattt cttcaacccg
     6181 cagttcccac acatggcagc catcttctgg attctggctg gcgggatcgc cgcaatcgca
     6241 tcgccggtgg gttacgcgcg agcgcggcac agggtatcct ccgacataac gcaggagcgg
     6301 tggacgagtc gccggcggct gaggcaggga ctggcaacca ggaacggggg atgaggatgg
     6361 ctggacgaca gattgagggg atcttcgcgc cggtcgcgac gctgtttgac agcgacggcg
     6421 agatggatct cgcgaagtac cgcaagaaca tggagtggta cgccggatcg ccgctggacg
     6481 gcgtcgtcat catgggctcc aacggcgaat atgcgtcgct cgataccgac gagaagctgc
     6541 ggctgatcga ggccgggacc gaggcgatcg gtggccgcaa gaaggtgctg gtcgggaccg
     6601 gcgtcgaatc gacgcgaggc acgatccggc tgacgcgagc ggcggctgag atgggtgccg
     6661 actacgcgct ggttgtcacg ccgtactact accggccgcg ctacgacaag gcagccttcc
     6721 tgcaacatta ccaggcggtc gccgaggcgt cgccgattcc ggttctgatc tacgtcatga
     6781 cggcctacac cggcgtcgat ctgccgtcgg cggtcgtcgc tgagctgtcg cgccacccta
     6841 acatcgttgg cgtcaaggac agcgccggaa acgcagtaaa gttctcggag atggtctccg
     6901 attcggacga cgatttctcc gttctggctg gctcggccaa cttcctctat ccggcgctct
     6961 gtctcggcgc tgccggcggc atcctggcgc tggccgacat tgcaccggct gcctgcgtcg
     7021 aggttcgcga tctgttcgcg gcaggcgacc acgagcgggc gcgggaggca cagttcaacc
     7081 tgctggcgcc gaacgcggcc gtgacgacac gcttcggcat cgccggtctg aagtcggcac
     7141 tggcaatggt cgggctggag acgagcgatc cgcgaccgcc gatgctgcca tcgaccgatg
     7201 aggagcgcgc gcagatccgc gcgatcctcg agcgagccgg aatccttgcc cgagtttgag
     7261 tggctggcac acccgatccg gatcgggttg gtcgccgaca cccaccggcg caacccgaag
     7321 cggggattgc cgccggcgct gctggacggc ctgaccggct gcgacatgat cttccacgcc
     7381 ggcgacatca gcgagcgctg ggtgctggat ctgcttgaag agatcgcgcc ggttcgagcg
     7441 gtgattggca acaacgactc attcgagctg ctgaagacgc tgccggaaga gcgctacttc
     7501 gtgtcgggca gtcaccgcat cgggctgctg cacggctaca gcgacatggc aaagaaggcg
     7561 acgaccgcga aggtctggac gctgggacgg atgcgcggcg tcgtcgattg cgccgtctat
     7621 gggcacagcc atcaaccgga gatcgccgag cgggaagggc tgctgatgat caaccccggc
     7681 tcgccgacgg atttcaggtg ggcaaaggga cccacatacg gcatccttga ggtaggcgag
     7741 gcaattcggg cgcgtttgat cgagctgtag aggtcggcca gcgagcgaca cccgaatacc
     7801 gggaacgaat ccgcgtagag aacggctcca aattgaggcc gtttgacgcg gttttcgtgt
     7861 tgcgttggtt ggacaacgcg aactataata acgactgcgt tccgccgaag gcacgacacg
     7921 atgttggcgc tgtcgcgcgc cgttcggcaa atctgacgcg cgaggatggt atatggaccg
     7981 tagtgacttt gacgaagccg cgacccgtct acaggggagt gtcggcttca aggacgacga
     8041 ggcggccgac gaagtcggac gctcgctcat ggagcagctg ctggctgatc cgtctcacga
     8101 ttacaggacc ctcaagtatg gcgatgtcgt cgacggcacc gtcatgcatg tcgatcggga
     8161 cgagatcctg gttgacattg ggtccaagtc ggagggtgtg atcccggcac gcgagttctc
     8221 aacgctggac gaccaggagc ggaagtcgat gggagtcggc gaccagctgc tggtgttcgt
     8281 ggttcagccg gagaaccagg atgggcacgc cgtcgtctcg atcgaccgtg cgcgtcagga
     8341 gaagagctgg cggatcctgc aggagcagta cgaggccggc caggtcatct cggccgaggt
     8401 tgtcaactac aacaagggcg gcctgctggt gaacctcgat ggtgtccggg gcttcgtccc
     8461 ggcttcgcaa gtcaccgaga tccgtggcgg agacgacgcc cagaagcagg ccgacatggc
     8521 ccgcctgatc ggcaacagcc tgcaactgaa gatcatcgag atcaaccgcc accgcaaccg
     8581 gctgatcctc tccgagcgcc aggctctcca ggagcggcgc gacgagatga aggagaagct
     8641 gatcgagcag ttgcgtgagg gcgaggttcg acagggccgc gtctccagta tctgtgactt
     8701 tggcgccttc gtcgacgtcg gcggtgctga cggactggtc cacctttcgg aactctcgtg
     8761 gagccgcgtc cggcacccat cggaggtgct gcacgtcggt caggaggtcg atgtcttcgt
     8821 cctcggcatc aacgccgagg agaagaagat cgcactctcg atcaagcgga cccaggccga
     8881 gccgtggagc agagtagcag ccaagtacga tgtcaatcag ctcgtcatcg gaacggtgac
     8941 ccagctcgcc aacttcggcg ccttcgccag gatcgaagac ggcatcgagg gtctggttca
     9001 cgtctccgag ctctccgatg cgcgcatctc tcacccgcgc cagctggtct cagagggcca
     9061 ggagctgttg ctgcggatca tccgcattga cccgcagcgg cggcggatgg gtctgagcct
     9121 gcggcgcgcg cttgaggcga gcgacgacga ggttcgcgag gcgcttggtg acgaggcggt
     9181 tgaactcaaa cagcgtctga tggcgctgga ggtcgagccg gatccggacg ccgagctgga
     9241 gccggagcgt gagacatcgc gcagtagcgc aatcgccgag ccggaagtcg ttgtcgagcc
     9301 agaaggtcca gcgacggcag tcgagcaggt tgaagtctcc aatgccatga ccgaggcgtt
     9361 tgctgcggcc gaggctgccg cagccgcgac caacggtaca gaagatgacg cacaagtagc
     9421 cgtgtccgat gtcgccgacg gtgacgaacc ggatgtggca gccgacgccg ccgaggcgga
     9481 ggtcgaaacg ccggtcgccg agaccgaaga ggtcgctgcg gcaggtgagc cggtcgctga
     9541 gagcgaagcg gcaacagttg ccgagtccgc tgccgagcag gcaccggagg aagtcgcagc
     9601 ctcggcttcg accgagaccg acgagaccgg cgcggtcagc gccgcccccg cttcagatga
     9661 tgagtccgag aagaggatga gctagcgctc agttctcggt catcagcctc cgaattccga
     9721 atcgagtgct ggcggccgga ccaaaagtcc ggccgctggt gatataacca gatgacaccg
     9781 gtgtgaatat cctcgccaat catggccgag gtcttgcgct ctgttgacac cggcagtacg
     9841 ctcttggcag gcagccaccg tagtacggcc cacgccaggc gtttgggcac cgtgccctcc
     9901 ccttgtctcg cgtggccgtc ggagagacca gatggcagat aaatttgaga agtttacgga
     9961 gcgtgcgcgc aaggtcctga ccttggcgca ggaagaggct caacgcttca accataacta
    10021 catcggcact gagcatttgt tgcttgggct ggtgcgggag ggtgacggcg ttgcggcgcg
    10081 ggtgctcagc aacatggggg tccaactccc caaggtgcgc tcggccgttg agttcatcat
    10141 cggccggggt gagtcggtca tcatgggcga gatcggcttg acgccgcgcg ccaaaaaggt
    10201 gatcgagctg gccgtggacg aggcccgccg actgaaccac cactacatcg gcaccgagca
    10261 cttgctgctc ggtctcgtgc gtgagggcga agggatcgcc gctggcgtgc tggagagcct
    10321 gggcgtcaac ctcgagaagg ttcgcgcaca ggtgatgcag gtcgtcagcc agaacgcggg
    10381 ctaccagcag tcgaagcagg cgaccaagac gccctacatg gatgcgcttg ggtttgacct
    10441 gaccgaggca gcccgtcaga acaagctcga cccgattatc ggtcgccaga acgagatcga
    10501 gcgggtcatg cagatcctct cgcgcaagac gaagaacaac ccggccctga tcggagagcc
    10561 cggcgtcgga aagacggcca tcgtcgaggg gctggcgcag aagatcatcg ctggagacgt
    10621 gcctgaactt ctgcagaaca agcggctcgt cgcactcgac atcggtgccc ttgtcgctgg
    10681 caccaagtat cgtggtgaat tcgaagaacg actgaagaag atcgtcgccg aggtcaagga
    10741 gacgggtgcg attctcttta tcgacgagtt gcatacactc gtcggtgccg gcgctgccga
    10801 gggcgccgtc gacgccgcca acatcctcaa gccggcgttg tcgcgcggtg aggtccagac
    10861 gatcggcgcg acgacgctgg acgagtaccg taagtacatc gagcgcgacg cggctctgga
    10921 gcggcgcttc cagccgatcc aggttccgga accgaatgtc gaagagacga tccagatcct
    10981 ctatgggatt cgcgaacggt acgaagagca tcacaagctc aagatcaccg acgagtcgat
    11041 cacggctgcg gccaacttcg ccgcgcgcta tgtgaccgac cgtttcatgc ccgacaaggc
    11101 gatcgatctg atcgacgagg cctcgtcgcg cgtgcgcatg taccgctctg cctcgccacc
    11161 cagcctcaag gaggccatgc gcgggatgga gagtctccag cgggagctgg acgcggcagt
    11221 cgctggccag gagttcgagc tggccgccga cctgcgtgac cgcgagcgca agctgcggat
    11281 gcggatcgcc gagctggaga aggactggaa ggaagagcag ggcagcgaga tgccggaggt
    11341 gtccgaggag gacatcgccc aggttgtctc gatgtggacc ggaattcctc tgacccggct
    11401 ggcggctgag gagtccgagc gcctgctgca catggaggcg gcgctgcacg agcggatcgt
    11461 tggacaggac gaggcgatct cgacgatggc gaaggccgtc cgtcgcgcac gtgccggcct
    11521 gaaggatccg cgccggccga tcggatcgtt catcttcctc ggcccgaccg gcgtcggtaa
    11581 gacgttgctg gctcgggcgc tggccgagtt catgttcggc tccgaggacg cgctgatcaa
    11641 gatcgatatg agcgagttca tggagcgcca caccatctcg cggctggtcg gagcgcctcc
    11701 gggctacgtc gggtacgaag agggcggtca actgaccgag gcggtgcggc gtaagtccta
    11761 ctcggtgatc ctgctggacg agatcgaaaa ggcgcatccg gaagcgttc
//
LOCUS       DASUGJ010000493         6769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_495156, whole genome shotgun sequence.
ACCESSION   DASUGJ010000493 DASUGJ010000000
VERSION     DASUGJ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495156"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFV93_11075"
     CDS             complement(<1..473)
                     /locus_tag="VFV93_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5165928.1"
                     /translation="MKTLSPFARVMHATAGVEIPLPLAIASIYGPLTLPAYAGRPLVI
                     SNFVTTLDGVVSLAVPGKEGGRAISGDNQHDRLLVATLAAVADAIVIGGGTLRSSPGH
                     IWTADALAPDFAAAFATLRQSLGKTGPPLNVVVTRDGDLDPAERLFQAGKVPVLVV"
     gene            complement(470..1129)
                     /gene="folE"
                     /locus_tag="VFV93_11080"
     CDS             complement(470..1129)
                     /gene="folE"
                     /locus_tag="VFV93_11080"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX5165929.1"
                     /translation="MSEPVTEIDDFPAIRTGDEAGLPRQLSPEQVRRFEGYAAEIFAA
                     CGMDLDTPSTRDTPRRFIRALYDITAGYDGDPKLIKVFDTECRGGSDCRLSQVIEGPI
                     RFYSLCEHHALPFFGQAYVGYIAHEQIIGISKLARLVRVVTQRFTVQERVSQQIIDTL
                     EELLQPHGVAVYLEAHHLCTEMRGVRETSPLTRTTYWRGEYDNNPSLRAEFFTACGLQ
                     R"
     gene            complement(1186..2379)
                     /locus_tag="VFV93_11085"
     CDS             complement(1186..2379)
                     /locus_tag="VFV93_11085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX5165930.1"
                     /translation="MPHFQYLLVGGGMTSAAAVTGIRTVDPDGSIGLIGQETDRPYAR
                     PPLTKGLWKGKSVDSIWRKSVNKGVELMLDRTATELDIQGKSVRDDHGAVVTFDKLLL
                     ATGGTPRRLPFGGDDIIYFRTLADYQRLREMSGPGKRIGVIGGGFIGSELAAALTMTD
                     TEVVMVFPDSSIGSRAYPADLAAFVTDYYREKGVTVLAERQATGLERSGPQLSLHVAG
                     ENGEQSETVAVDGVVAGIGIQPNVDLAERAGLPVDNGIVVDEFLRAGAPDIYAAGDVA
                     AFVNPALDRRIRVEHEDNAKAMGRQAGRNMAGAAEPYEYLPSFYSDLFDLGYEAVGDV
                     DARLETVADWTEPFRKGVIYYLQDGRVRGVLLWNVWDQVPAARELIAAPDPVKPEDLK
                     GRLPS"
     gene            complement(2543..3040)
                     /locus_tag="VFV93_11090"
     CDS             complement(2543..3040)
                     /locus_tag="VFV93_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5165931.1"
                     /translation="MYTRIVVPLDGSELAEWALPHGEELARVIGVPMHFIRVVDVVAT
                     AGFPVADFTYSDNLVFDARQSEERAAADYLARLRECYADRKFLVTVERRIGRVTEELL
                     AAARDGDLYVMASHGRGGVSRWFLGSVAEELTRHSRVPVMVIRAQTAKRRHAVPIPHV
                     AAHRR"
     gene            3773..4027
                     /locus_tag="VFV93_11095"
     CDS             3773..4027
                     /locus_tag="VFV93_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165932.1"
                     /translation="MRENTIPERPPEQNEEPSTGPFRAKDVRQEMPSNTERAGSDEID
                     IVDFQSMQSFPASDAPSWPSVSSEPPVNGASEQRESPETG"
     gene            4050..5054
                     /locus_tag="VFV93_11100"
     CDS             4050..5054
                     /locus_tag="VFV93_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5165933.1"
                     /translation="MPTDAVPDNTFEILAVAALRLIGDGSGTAELAERFRRLQIPREP
                     DDAAAMLDDLARRGLVRVSNTLQGEPTYVMTLLGQKYAGIQPDGTHVLSDELEELERL
                     RTDFISTIAHELRTPLTAVRTCVGLLLEYSVAPEPAIRTRLLENAARSADNMQRLVTD
                     LLDLARFRSGHIRLQARRFDACALAHDAGSILAPLAEALGQVLEIDVPDEPVWVYADR
                     RRLEQVLLNLLSNAQKFSPDSARIWLSLQATEEDVAWSVRDVGPGISPDDQAHLFERF
                     FTREPDAVARKAGAGLGLPIALAIAEAHGGTITVDSAIDRGSTFTLSIPRYTDVEAEE
                     "
     gene            5054..5746
                     /locus_tag="VFV93_11105"
     CDS             5054..5746
                     /locus_tag="VFV93_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5165934.1"
                     /translation="MKILLVDDAPEVVESIRLGLMLLWRDVEVLDAGTGNATLDLVER
                     ETPDLVLLDIGLPDMNGFDVLREIRAFSDLPVIMLTAQDNTMDKVKGLELGADDYITK
                     PFDHLELLARIQAVLRRLDMSAPKSRTPSFRSGDLEVNFSAQEAHVNGVRLELTATEY
                     KLLYHLVRNAGQILPHGTLLARVWGREYVDEVDYIRVYIRRLRAKLGDDPERPRYIQT
                     ERGLGYRFLLPR"
     gene            5873..>6769
                     /locus_tag="VFV93_11110"
     CDS             5873..>6769
                     /locus_tag="VFV93_11110"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX5165935.1"
                     /translation="MDAREPHAFTSHRCSDGQPEIGSSMLPESDVPGGVHIQMLGGFA
                     VTVGGWTVPESIWRQRRAAGIIKLLALEPGHRLHREQLMDLLWPDLDYTAQLNNLRQA
                     LHHARRGLESAGMPAGTALERDGNIVVLARNVQVQVDVDAFEMAVTSAWQQRTPAAAE
                     AALALYGGDLLPDDRYEEWAEQRRTTLRASYLALLARLAAFYDERGEHADSIGAWQRL
                     VAADPTNEAANLELMRHYATTGQRQLALAQFDQLMMALERDLDTSPEPATCELAEAIR
                     KGRFPEAGPPDAPAAPDTPATPH"
BASE COUNT         1201 a         2228 c         2237 g         1103 t
ORIGIN      
        1 acgaccagca ccggcacctt gcctgcttga aacaggcgtt cggctgggtc gagatcgccg
       61 tctctggtca cgacgacgtt cagcggcggg ccggtcttgc cgagcgattg gcgcagcgtc
      121 gcgaacgcgg cggcgaagtc cggagcaagg gcgtcggccg tccagatgtg ccccggtgat
      181 gagcgcagcg tcccgccgcc gatcacgatc gcgtcggcca cggcagccag cgtcgcgacc
      241 agcagccggt catgctggtt gtcaccgctg atcgcccgcc cgccttcctt gccgggcacc
      301 gccagcgaca cgacgccgtc gagggtcgtg acgaagttac tgatgacaag cgggcgaccg
      361 gcatacgcag gcaaggtcag cggcccgtag atcgacgcga tcgccagcgg gagcggtatc
      421 tccacgccgg ctgtcgcgtg catgacgcgc gcaaacggcg acagcgtctt caccgttgca
      481 ggccgcaggc ggtgaagaac tcggcccgta acgacgggtt gttgtcgtac tcgccgcgcc
      541 agtaggtcgt gcgggtgagc ggcgatgtct cacgcacgcc gcgcatctcg gtgcagaggt
      601 gatgcgcttc caggtagacg gcgacgccgt gtggctggag cagctcttcg agcgtgtcga
      661 tgatctgctg gctgacacgt tcctgaacgg tgaagcgctg ggtgacaacg cgcacgaggc
      721 gcgccagctt cgagatcccg atgatctgct cgtgcgcgat gtaaccgacg taggcctgcc
      781 cgaagaacgg cagcgcgtga tgctcgcaga ggctgtagaa gcggatcggc ccctcgatga
      841 cctggctgag ccggcaatcc gacccgccgc gacactcggt gtcgaacacc ttgatcagct
      901 tggggtcacc gtcgtagccg gcggtgatgt cgtagagtgc ccggatgaag cgccgcggtg
      961 tgtcacgcgt cgacggcgtg tccaggtcca tcccgcaggc tgcgaagatc tcggccgcat
     1021 agccctcgaa gcggcgaacc tgctcgggac tcagctggcg cggcagaccg gcttcgtcgc
     1081 cggtccggat agcggggaag tcatcgatct cagtcactgg ttcagacacg attgttgcgc
     1141 tcctgtgcgt ccacggcggc gtctcatctc gggtcagcga caccgttagc tgggcagccg
     1201 ccccttgagg tcctccggct tgaccggatc tggcgcggcg atcagctcgc gggcggctgg
     1261 aacctggtcc cagacattcc agagcaggac accccggacg cgcccgtcct gcaggtagta
     1321 gatcacgccc ttacggaacg gctcggtcca gtcggcgacc gtctcgagcc gggcgtcgac
     1381 gtcgccaacg gcctcgtagc cgaggtcgaa gagatcggag tagaaggacg gcaggtactc
     1441 gtacggctct gccgcgccgg ccatgttgcg tccggcctgc cggcccatcg ccttggcgtt
     1501 gtcctcatgc tcgacgcgaa tgcgccggtc gagggccggg ttgacgaagg ctgccacatc
     1561 gccggcggcg tagatgtccg gggcgccggc gcgcaggaac tcatcgacga cgatcccgtt
     1621 gtcgaccggc aacccggccc gctcggccag atcgacgttc ggctggatgc cgatcccggc
     1681 gacgacgccg tcaaccgcga ctgtttctga ctgctcgccg ttctcgccgg cgacgtgcag
     1741 cgagagctgg gggccggatc gctccaggcc ggtggcctgt cgctcagcaa gaacggtgac
     1801 gcctttctcg cggtagtagt cggtgacgaa ggcggcgagg tcagccgggt aggcgcggct
     1861 gccgatgctg gagtctggaa agaccatgac aacctcggta tcggtcatgg taagtgcggc
     1921 ggccagttcg gagccgatga acccgccgcc gatgacgccg atgcgcttgc ccggcccgct
     1981 catctcgcgc aggcgctggt agtcggccag cgtccggaag tagatgatgt cgtcgccccc
     2041 gaacggcagc cggcgcggcg tgccgcccgt tgccagcagc agcttgtcga aggtcacgac
     2101 tgcaccatgg tcgtctcgca cggatttgcc ctggatatcc agttcagtcg cggtccggtc
     2161 gagcatcagc tcaacgccct tgttgacgga cttgcgccag atgctatcga ccgatttgcc
     2221 cttccagagt cctttggtca ggggcgggcg agcataaggc cgatcggttt cctgaccgat
     2281 cagcccgatc gagccgtccg gatcgaccgt gcggataccc gtaacagcgg cggctgatgt
     2341 catcccaccg ccgacgagca gatactgaaa gtgcggcatt cgattatcct cccgcgcatg
     2401 tctgcgcaac gagcgtctgt agttggcggc cggtgcggcc tggccggccg ctggggcgca
     2461 gcctgctcca cgctgcgaag catcgctatc tgatacgaaa cgagatgccg gaccgggttg
     2521 caccgcgcca gacggccgcc ggctatcggc ggtgcgccgc gacgtgcggg atcggcaccg
     2581 cgtgccggcg ctttgctgtc tgggctcgaa tcaccatcac cggaacccgc gagtgccggg
     2641 tgagctcctc ggcgacactg cccaggaacc agcgcgagac gccaccgcgt ccatgcgatg
     2701 ccatcacgta cagatcgccg tcgcgcgcag ccgccagcag ctcctccgtc acccggccga
     2761 tccggcgctc gaccgtcacg aggaacttcc gatctgcgta gcactcgcgg agcctggcca
     2821 ggtagtcggc agcggcccgc tcctcgctct gccgcgcgtc gaagaccagg ttgtcgctgt
     2881 aggtgaagtc ggcgacgggg aatccagccg tcgccaccac gtcgacaacg cggatgaagt
     2941 gcatcgggac accgatcacg cgggcgagct cttcaccatg cggcaacgcc cactcggcca
     3001 gttccgaccc atccagcggg acaacgattc gcgtgtacat cgccgcatcc attccaatgc
     3061 atttgcgcgt cgagccggtc tggtgatcgg ccatctccga cgatgccatg aatcacgatc
     3121 gcggtcggta actaggcctg gcagtgacgg tcgcaagaac catatcgtca cgcccgttga
     3181 acgggacaag gggagaggcg ggtggggtgg cccgcctcca tggaaaaggg ggacgcgtgg
     3241 gagaagatct ggggtgcttg cgggggcgat tggccatcaa gcgcgccaga agaagcgatg
     3301 cgagccggtc gaagacatgt aaacgcatgc cattcccctt ccgggtatct ctgccgtcgt
     3361 tcccgctggc ggcgctggtg gacagttcgt gtaccctgct cccacactac tcctgctcca
     3421 tgaacgggcc gcttgcgtcg catgaacgtt tcgtgaacag ccgggattgc cgaaacaggt
     3481 aagcgttcac gtggcgttca tgcacgtcca tgtcatttgt cagaggcggg atcgatactc
     3541 ggaggatggc agaggtggcc atgacgtcag gctggcacgc cacggcgact aacgaggacg
     3601 ttccggtgtc cgaccgacgc agtcgacagg cgacgacgct tttcgacggc cggacgtgcc
     3661 agcgggcagc ggcagaacaa ccactcgcgt cctgccgctg cgccgattgt cgcgaacgac
     3721 gtacgataga cgagcgacgg cgcgttcgag cgacgcgaag gagtggatat tcatgcgtga
     3781 gaacaccatc cccgagcggc caccggagca gaacgaggag ccgtcaaccg ggccgtttcg
     3841 cgcgaaagac gtacggcagg agatgccgtc caacaccgaa cgcgccggca gcgacgagat
     3901 cgatatcgtc gactttcagt cgatgcaatc cttcccggcc agcgatgcgc cctcgtggcc
     3961 gtccgtgagt tctgaaccgc cggtgaacgg ggcgagcgag cagcgggaat cccccgaaac
     4021 cggctagccg gaagagactg acagctgcga tgccaacgga tgctgtgccg gacaacacgt
     4081 tcgagatcct ggcagtggcc gccctgcgcc tgatcggtga cggcagcggg acagccgagc
     4141 ttgccgagcg tttcaggcgc ctgcagatcc cccgcgagcc ggacgacgca gccgccatgc
     4201 tcgacgatct cgcgcggcgg gggctcgtcc gggtgtccaa cacgctccag ggcgagccga
     4261 cctacgtgat gacgttgctg gggcagaaat acgccggcat ccagcccgac ggcacccacg
     4321 tgctgtcgga tgaactggag gagctggagc ggctccggac cgacttcatc tcaaccatcg
     4381 cccacgagct gcgaacaccg ctgacggccg tgcgaacctg cgtcggcctg ctgcttgagt
     4441 actcagttgc gccggagccg gctattcgga cccggttgct ggaaaacgct gctcgaagcg
     4501 ccgacaacat gcagcggctg gtcaccgatc tgctcgatct cgcgcgcttt cgcagcggcc
     4561 acatccgact ccaggcgagg cggttcgatg cctgcgcgct ggcgcacgat gccggctcga
     4621 tactcgcccc gctggcagag gcgctcggtc aggtgctgga gattgacgtt ccggatgagc
     4681 ctgtctgggt ctatgcggac cgccgccggc tcgaacaggt gctgctgaac ctgctctcaa
     4741 atgcgcagaa gttctcgccg gatagcgcgc gaatctggct ctcgctccag gccactgaag
     4801 aggatgtcgc ctggtcggtc cgcgacgttg gccccggcat cagtccggac gatcaagcgc
     4861 acctgttcga acgcttcttc accagggaac cggatgccgt cgctcgcaaa gccggcgcgg
     4921 gactcggcct gccgatcgcg ctggccatcg ccgaggcgca cggcgggacg atcacagttg
     4981 acagcgccat cgatcgcgga agcacattca cgctgtcgat cccccgctac acagacgtgg
     5041 aggccgaaga atagtgaaga tcctgctggt cgacgacgcg ccggaagtgg tcgaatccat
     5101 acggctgggg ctgatgctgc tgtggcgcga cgtcgaggtt ctcgacgccg ggaccggcaa
     5161 cgccacgctc gatctggtag agcgcgagac gccggatctg gtgctgctcg acatcgggtt
     5221 gccggacatg aacggtttcg atgtgttgcg cgagatccgg gcgttctccg atctgccggt
     5281 gatcatgctg acggcccagg acaacacgat ggacaaggtc aaggggctgg agctcggcgc
     5341 cgacgactac attacgaagc cgtttgacca cctggaactc cttgctcgca tccaggcggt
     5401 cctgcgccgg ctcgacatgt ccgccccgaa gagccgaacg ccgtccttcc gctccggcga
     5461 tctggaagtg aacttctccg cgcaggaagc gcacgtcaac ggcgtgcggc tggagctgac
     5521 ggcgaccgag tacaaactgc tctatcacct cgtgcgcaac gccggccaga tcctgccgca
     5581 cggtacgctg ctggcccgcg tctgggggcg agaatacgtc gacgaggtcg actacatccg
     5641 cgtctacatc cgccgcctgc gcgccaagct cggcgacgat cctgagcgcc cccgctacat
     5701 tcagaccgag cgcggtctcg gctaccggtt cctactgccc agataagccg gcggctggta
     5761 tcctctcccc atcagaatac gccagaatgc aggcaaagac gcgcgggtcg tggaccgcgc
     5821 ggtctgagtt cgaccgaccc gaccgggcaa tatccgacgc gatggatggc cgatggacgc
     5881 cagggagccg cacgccttca cctcccatcg atgttccgac gggcaacccg agatcggctc
     5941 gtcgatgctg cccgaatccg atgttcctgg cggtgtgcat attcagatgc tgggtggatt
     6001 cgccgtcacg gttggcggct ggacggtccc agaatcgatc tggcggcagc gccgggcggc
     6061 gggcatcatc aagctgctcg cactcgaacc ggggcaccgg ctccaccgcg agcagctgat
     6121 ggacctgctc tggccggatc tcgactatac cgcccagctc aacaacctgc ggcaggcact
     6181 gcaccatgcc aggcgaggcc ttgaatcggc cgggatgccg gctgggacgg cactcgaacg
     6241 cgacggcaac atcgtcgtcc tcgcgagaaa cgtgcaggtc caggtcgatg tcgatgcgtt
     6301 tgagatggcc gtcaccagcg cgtggcaaca acgcacgccg gccgccgccg aggcagccct
     6361 ggccctgtac ggcggcgact tgctgcctga cgatcgctac gaggagtggg ccgagcagcg
     6421 ccggacaacg ctgcgcgcca gctacctcgc attgctggcg cgtctggcag ccttctacga
     6481 cgaacgcggc gaacacgcgg attcgatcgg tgcctggcag cgtctggtcg ccgctgaccc
     6541 aaccaacgaa gcagccaatc tggagttgat gcgccactat gcaacaaccg gccagcgaca
     6601 actcgcgctg gcgcagttcg accaactgat gatggcactc gaacgggacc tggacacgtc
     6661 gccggagccg gcaacctgtg aactggccga ggcgatccgc aagggccgct ttccagaggc
     6721 aggcccaccg gacgcaccgg ccgcccccga tacacccgca acgccgcac
//
LOCUS       DASUGJ010000494         4029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1327599, whole genome shotgun sequence.
ACCESSION   DASUGJ010000494 DASUGJ010000000
VERSION     DASUGJ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327599"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..1009
                     /locus_tag="VFV93_11115"
     CDS             284..1009
                     /locus_tag="VFV93_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165936.1"
                     /translation="MAAVLISGPVAIIAPQSASAAFCGNCYGTAFWYGNVNGADARIK
                     MDDLLSATSSNTNYHVGNTLWVTDTDIGSQTCFTGGIYAHTGWVEIGYEARANTYIFY
                     TADCRPNGSFARWPLASVQVTDYDAANYFEIFNSGYNQWYVSVTADSGLYDQNWYSNS
                     IQPNMIEIGIEAAGSSLHADRANFTNNRYIQGPNRYYQFGHDSQNTTLLGNPGSGNPP
                     RSRWVTTPAPGNNGGNWTTSCAC"
     gene            1041..1607
                     /locus_tag="VFV93_11120"
     CDS             1041..1607
                     /locus_tag="VFV93_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165937.1"
                     /translation="MKRSTHFIMVGIGVLTITGLIALASGDQISGGADVIAGDLNQPT
                     PTVEGGVPLQPSDLPRTDTYSPGIEAIPVTNPLNGDGPAFTEDAVQAYLEANPPAGWD
                     PTTPQPEIISIQFLLTEEAEVVVDHTIPRKPQDLLCIATVRGTFVSELPPSIAATTET
                     EATQSTLVVIFDAITGNLIGEVSLREPQ"
     gene            complement(1695..2687)
                     /gene="fdhD"
                     /locus_tag="VFV93_11125"
     CDS             complement(1695..2687)
                     /gene="fdhD"
                     /locus_tag="VFV93_11125"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX5165938.1"
                     /translation="MLVPEPADHVERRVARIRDGVWTDAWDTLAAEEPMEIRLRWWEG
                     TQERRESIAVTMRTPGDDFELAAGFLHGEGIVTGRDDIVDVSWCLDGLAEGEPQTFNI
                     VTVTLRPDVPFDIARLDRHFYTTSSCGICGKATLESLEVRGCDPLPSGPSVDPAIIQS
                     LPAALRRSQEVFERTGGLHAAGLFDRDGTLLALHEDVGRHNALDKLIGTQLLAGNCPL
                     DGCLLLLSGRASFELLQKALVARIPVVAAVGAPSSLAVSLAESFNMTLLGFVRPDGFN
                     VYTGVERIALSVSSAERGDASDPENVFDGLARHAEQEYLSGRTSNLRESLGKGT"
     gene            complement(2674..3663)
                     /locus_tag="VFV93_11130"
     CDS             complement(2674..3663)
                     /locus_tag="VFV93_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5165939.1"
                     /translation="MTVTIPLTPVRRTRLTRMIPLMIDGRDVSAPEGSTILDACRDLG
                     IETPTLCYGDTLTPVNACRVCVVELEGSRTLVPACSRKVEPGMKILTDSERVRHSRKL
                     VLELLGSSVDFSYAPAETQDYFGRYDAEPERFGPAAAPAALGERDHAHTGHHELDPVG
                     LAATVDQPPKIDNELYIRDYSRCILCYKCVEACGTDHQNTFAISIAGRGFDARVSTEY
                     DTWLPDSACVYCGNCIAVCPTGALMFTTEYELRQEGKWDESRQTVTDTICPYCGVGCT
                     LELHVQDNQIVKVTSPMDHDVTLGNLCVKGRFGWQHVQNRAEPSANGADGAGS"
     gene            complement(3660..>4029)
                     /locus_tag="VFV93_11135"
     CDS             complement(3660..>4029)
                     /locus_tag="VFV93_11135"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5165940.1"
                     /translation="FDVPLTFEGTRAIGGSLGSGVIMVFDDTANMPDILLRIAAFFRD
                     ESCGQCVPCRVGTVRQQEALRRLASGQPLGTVAGELQLIDDVGMVMREASICGLGQTA
                     ANAIQSGIAKLHLFQNGNLR"
BASE COUNT          758 a         1267 c         1251 g          753 t
ORIGIN      
        1 cgagaccggt atcgccgaag agggccgcca ccaggaactg ctcaagaccg aagggctcta
       61 ccaacgcctg catgccgccc agttcgcgtc gtatgccgcg agctgacatg tcgtgatgct
      121 tcgcgccttc actgagaggt cgcctgtagg tgtcccgctt gacggattgc cttgcgttcg
      181 ttcacacgat ctaatgcggc tcaatagttc gttttcgtac tcgcgggatg gaggcagaaa
      241 tgtttgacta ccgtcgacgc gtatcgcggt cgttgctggt acgatggcag cggttctcat
      301 ctccggaccg gtcgccatca tagctcccca gtccgcttcc gcagcctttt gcggcaactg
      361 ctacgggacc gccttttggt atgggaatgt gaacggcgcg gatgcccgta tcaaaatgga
      421 cgacctcttg tccgcgacga gttcaaacac gaactatcat gttggcaaca ccttgtgggt
      481 gactgacact gacatcggta gccaaacctg cttcaccggc ggcatctacg cacacacagg
      541 atgggtcgaa atcggttacg aggctcgagc aaacacttac atcttctata cggcagattg
      601 cagacccaat ggctcgttcg ccagatggcc gttggcttcc gtacaggtga cagactacga
      661 tgccgccaac tacttcgaga tcttcaactc cggttataac cagtggtatg tgagcgtgac
      721 ggcagacagc ggtctgtatg atcagaactg gtacagcaac tcgatccaac cgaacatgat
      781 tgagatcggt atcgaagcag cgggcagttc cctacacgct gaccgtgcta acttcaccaa
      841 caatcgctac attcaagggc caaaccgcta ttaccagttc gggcacgata gccagaacac
      901 taccctgcta ggtaatccag ggagtggcaa tccacctcgg tcgcgttggg tcaccactcc
      961 ggctcccgga aacaacggtg ggaactggac cacatcctgc gcttgctagc gtagcttgct
     1021 cggccttcga ggagagttca atgaaacgtt caacgcattt catcatggta ggtattggtg
     1081 tcttaaccat cactgggctg atagctctcg cgtcgggtga tcagatctcg ggtggagcag
     1141 atgtcattgc cggcgatctg aaccagccaa cccctacggt tgagggtgga gttccattgc
     1201 agccttccga tctgccgcgg acggatacat actctccggg catcgaggcc attcctgtaa
     1261 cgaatcctct taacggcgac ggtccagcct tcaccgaaga tgctgtccaa gcgtatttgg
     1321 aagcgaaccc tccggccggg tgggatccga cgacacctca acccgaaatt atcagcattc
     1381 agttccttct cacagaagaa gctgaagtcg tagtcgacca cacgattcca cgaaagcccc
     1441 aggatctgct ctgcatcgcc actgtccggg ggacattcgt ctcggaactg cctcccagca
     1501 ttgcagcaac cacagagaca gaggctactc aatcgacgct cgtcgtgatc tttgacgcca
     1561 tcacgggcaa cctgatcgga gaggtctctc tccgagaacc acagtagtcc atcaatgctt
     1621 cgtattccgc ggagcatcca tcgctccgcg agaggttgac gatcctaggg tactgattcg
     1681 ccttgttcga ttgctcaggt tcccttaccc aaagactcgc gcaggttact cgtcctgcct
     1741 gataggtatt cctgctcggc atgacgagca agcccatcaa acacattctc agggtcggag
     1801 gcatcgccac gttcggcgct tgagacagat agagcgatcc gctccacgcc agtgtagacg
     1861 ttaaacccat ccggccgcac aaaccccagc agggtcatgt tgaacgactc ggccagcgag
     1921 acggccaggc tgctgggggc gccaaccgcc gccacaactg gtatccgggc gacgagcgct
     1981 ttctggagca gctcgaagct ggcccgaccg ctgaggagga gcaggcagcc gtccagcggg
     2041 cagttgccgg ccagcagttg cgtgccgatc agcttgtcga gcgcgttgtg gcggccgacg
     2101 tcctcgtgga gcgccagcag cgtgccgtcg cggtcgaaga ggccggcggc gtgcaggcca
     2161 ccggtgcgct cgaagacttc ctgtgagcgg cgtagcgcgg ccgggagcga ctggatgatg
     2221 gccgggtcga cgctcgggcc ggacggcagc ggatcgcaac cgcgcacttc cagcgactcc
     2281 agcgtcgcct tgccgcagat gccgcaactc gaggtcgtgt agaagtgccg gtcgagccgg
     2341 gcgatgtcga acggcacgtc gggccgcagc gtgacggtga cgatgttgaa ggtctgcggc
     2401 tcgccctcgg cgaggccgtc caggcaccag gagacgtcga cgatgtcgtc gcggccggtg
     2461 acgatccctt cgccatgcag gaaaccggcc gccagctcga agtcgtcgcc gggggtgcgc
     2521 atcgtgacgg cgatgctctc gcgccgctcc tgggtgcctt cccaccagcg cagccggatc
     2581 tccatcggct cctcggcggc aagcgtgtcc caggcgtcgg tccagacgcc gtcgcggatg
     2641 cgggcgaccc gccgctcgac gtgatcggcc ggctcaggaa ccagcaccgt ccgctccgtt
     2701 cgcgctcggc tcggcccggt tctggacgtg ctgccagccg aagcggccct tgacgcagag
     2761 gttgccgagc gtgacatcgt ggtccatcgg cgaggtcacc ttgacgatct ggttgtcctg
     2821 gacatgcagc tccagcgtgc agccgacgcc gcagtaggga cagatcgtgt cggtcacggt
     2881 ctggcgcgac tcgtcccact tgccttcctg gcgcagctcg tactcggtcg tgaacatcag
     2941 cgcgccggtc gggcagacgg cgatgcagtt gccgcagtag acgcaggcag aatcgggcag
     3001 ccaggtgtcg tactcggtcg agacgcgcgc atcgaagccg cgaccagcga tcgagatcgc
     3061 gaacgtgttc tggtggtcgg tcccgcaggc ttcgacgcac ttgtagcaaa ggatgcagcg
     3121 cgagtagtcg cggatgtaga gctcgttgtc gatcttcggc ggctggtcaa ccgtcgccgc
     3181 cagcccgacc ggatcaagct cgtgatggcc ggtatgtgcg tggtcccgct cgccgagggc
     3241 ggccggcgcg gcggctggtc cgaagcgctc cggctcggcg tcgtagcggc cgaagtagtc
     3301 ctgcgtctcg gccggggcgt aggagaaatc gaccgacgag ccgagcagct ccagcaccag
     3361 cttgcggctg tggcgcaccc gctcgctgtc ggtcaggatc ttcatgcccg gctcgacctt
     3421 gcgcgagcag gccgggacga gcgttcggga tccctccagc tcaacgacgc agacgcggca
     3481 agcgttgacc ggcgtcagcg tgtcgccgta gcagagcgtc ggtgtctcga tgccaaggtc
     3541 gcggcaggcg tcgaggatcg tcgatccttc gggtgcgctg acatcgcggc cgtcgatcat
     3601 cagcgggatc atccgggtca atcgtgtccg gcggaccggt gtcagcggga tcgttaccgt
     3661 catcgcaggt tcccattctg gaagagatga agcttggcaa tccccgactg gatcgcgttc
     3721 gccgccgtct ggccgaggcc gcaaatcgac gcctcccgca tgaccatgcc aacatcgtcg
     3781 atcagttgca actcgccggc gaccgttccc agcggctggc cgctcgccaa tcgccgcagc
     3841 gcctcctgct ggcggacggt tccgacccgg cagggcacgc actggccgca cgactcatcg
     3901 cggaagaagg cggcgattcg cagcaggata tcgggcatgt tggccgtgtc gtcgaagacc
     3961 atgatgacgc cggagccgag cgagccgccg atcgcccgcg tcccttcgaa ggtgagcggc
     4021 acgtcgaac
//
LOCUS       DASUGJ010000495         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_632615, whole genome shotgun sequence.
ACCESSION   DASUGJ010000495 DASUGJ010000000
VERSION     DASUGJ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632615"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFV93_11140"
     CDS             complement(<1..881)
                     /locus_tag="VFV93_11140"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165941.1"
                     /translation="MLTKARLVEIKWDANQQTAESPPGAKSVEVDFNPQTLKLSYANE
                     NKGGDQPGGSNKQFVGSGTSKLGVELVFDTTGTGVDVRKRTMDVAYFIQAKAQKDKKN
                     TRVPPGISFEWGSFIFRGVVNSISETLEYFSPEGVPLRATVSLDISRQEIEYMFGKPG
                     QASAGPATSPKAGTPGQAPLDTPRPGESLQQMAARNGNSADWRAIAAANNIDDPLRLS
                     AGALIDVNVSAGVSASAGAGISIGAGASASASAGISIGGGAAAGISGGAAVGFSAGLG
                     GGVGFSSSGGIGAGAGIG"
     gene            complement(898..1371)
                     /locus_tag="VFV93_11145"
     CDS             complement(898..1371)
                     /locus_tag="VFV93_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX5165942.1"
                     /translation="MANEEYPFTTFNFEVQIEIPDAAGLGVSSPLCNSEFAEVDGLEM
                     TMEPKTLREGGNNTRQVHLIGPTSYGNLTLKRGMTTNQDIWVWFQSVINNTRPGSLAT
                     GVILMKDGAGTPVVRYTISDCLPIKLKAPALNAKDGILAIEELQIAYGAFTVERV"
     gene            complement(1375..>2424)
                     /locus_tag="VFV93_11150"
     CDS             complement(1375..>2424)
                     /locus_tag="VFV93_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165943.1"
                     /translation="SFGGVPASDVVVLSGVELRCTTPTAGVAGPTAVEVTTSAGTGTM
                     LNGFRYLPETVEPVLPELAPLGDYDIDAESSPLLPLQRELLRCCVARGDAVAILTLPR
                     HFEKRDCIDWQQALRGYLGLPRRRSVFNEVREIADLSYAAVYHPWLLIRDPGAADAMR
                     AVVADGAVCGMIAARERERQVWVAPANLQLEGVLGLVPAIPEGDWAELFEAQFNLVRG
                     EPRDFRPMSAHTLSDEAILLQLSVRRLMILLRKVAYLRGLDYVFESNHEHFREGVRAV
                     LSEMLRFMFERGAFAGPTPDSSYRVVTDERVNPPESVDQGRFIAEIQVAPSQPAEFIT
                     VVLSRVGDQLQLTEG"
BASE COUNT          370 a          847 c          786 g          421 t
ORIGIN      
        1 ccaattccgg caccggcccc gatgccgccg cttgagctga agccgacgcc gccgcccagg
       61 ccggcgctga agccgacggc cgctccgccg ctgatgccgg ctgctgctcc gccgccaatc
      121 gagatgccgg cgctggcgct cgccgacgct cccgcgccaa tgctgatacc ggctccggcg
      181 ctcgcggaga ctcccgcgct gacgttgacg tcgatcagtg cgccggcgct cagccgcagc
      241 gggtcgtcga tgttgttggc ggccgcgatc gctcgccagt cggcgctgtt gccgttacgg
      301 gctgccatct gctggaggct ctcgcccggt cggggtgtgt cgagcggggc ttggcccggc
      361 gtcccggcct tcgggctggt ggctggaccc gcgctcgcct ggccgggctt gccgaacatg
      421 tactcgatct cctggcggga gatgtcgagc gagacggtcg cccgcagcgg gacgccctcc
      481 ggcgagaaat actccagcgt ctcgctgatc gagttgacga cgccacggaa gatgaagctg
      541 ccccactcga agctgatgcc gggcgggacg cgcgtgttct tcttgtcctt ctgcgccttg
      601 gcctggatga agtaggcgac gtccatcgtc ctcttgcgga catcgacgcc ggtgccggtc
      661 gtgtcgaaga ccagctcgac gcccagcttc gaggtgccgc tgccgacgaa ctgcttgttc
      721 gagccgcccg gctggtcgcc gcccttgttc tcgttcgcgt agctcagctt gagcgtctgc
      781 gggttgaagt caacctcgac cgacttcgcg ccgggcgggc tctcagccgt ctgctggttg
      841 gcgtcccact tgatctcgac cagtcgcgcc ttggtcagca tcgtctcccc gcctccctca
      901 gacccgctcg accgtgaacg cgccgtaggc gatctgcagc tcctcgatcg cgaggatgcc
      961 gtccttggcg ttcaacgctg gcgctttcag cttgatcggc aggcagtcgg agatcgtgta
     1021 gcggacaacc ggcgtcccgg cgccgtcctt catcaggatc acacccgtcg ccagcgatcc
     1081 gggccgggtg ttgttgatga ccgactggaa ccagacccag atgtcctggt tggtcgtcat
     1141 cccgcgcttg agcgtcaggt tgccgtagct ggtcggcccg atcagatgca cctggcgggt
     1201 gttgttgccg ccctcgcgca gcgttttcgg ctccatcgtc atctcaagcc catcgacctc
     1261 ggcgaactcg gagttacaga gcgggctgct gacgcccagc ccggccgcgt ccggaatctc
     1321 gatctggacc tcgaagttga aggtcgtgaa tggatactcc tcgttcgcca tgccctaccc
     1381 ctccgtcagc tggagctggt cgccgacccg gctgaggacg acggtgatga attcggccgg
     1441 ctgggatggc gcgacctgga tctcggcgat gaagcgaccc tgatcgacgc tctccggcgg
     1501 gttgacacgc tcgtcggtga cgacgcggta cgacgagtcc ggcgtcggcc ccgcgaacgc
     1561 gccccgctcg aacatgaagc gcaacatctc gctgagaacc gcccgcactc cctcgcggaa
     1621 gtgctcgtga ttggactcga agacgtagtc cagcccgcgc aggtaggcga ccttgcgcag
     1681 caggatcatt agccgccgga ccgagagttg cagcaggatc gcctcgtcac tgagggtgtg
     1741 ggcgctcatc ggccggaaat cgcgcggctc gccacggacc aggttgaact gcgcttcgaa
     1801 caactcggcc cagtcgcctt ccgggatggc cggaacaagc ccgaggacac cttcgagctg
     1861 caggttggcc ggcgcgaccc agacctgccg ctcgcgctcg cgcgcggcga tcatcccgca
     1921 aactgcgcca tcagcgacga cggcccgcat cgcgtccgct gcccccggat cgcggatcag
     1981 caaccagggg tggtagacgg cggcatagct caggtcggcg atttcgcgca cctcgttgaa
     2041 gacgctacgc ctccggggta gccccagata gccacgcaac gcctgctgcc agtcgatgca
     2101 atcccgcttc tcgaaatggc gcggcagcgt caggatcgcg accgcatcac cacgggcaac
     2161 gcagcagcgc agcagctcgc gctgcaatgg cagcaacggg ctggactccg cgtcgatgtc
     2221 gtaatcgccg aggggcgcaa gctccggcag gaccggttcg accgtctccg gcaggtagcg
     2281 gaacccgttc agcatcgtgc cggttccggc gctggtcgtc acttcaacgg ccgtcggtcc
     2341 ggcgacgcct gccgtcggcg tcgtgcaacg cagctcgaca cccgacagca cgacaacatc
     2401 gctcgccgga acaccgccga agct
//
LOCUS       DASUGJ010000496         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_355968, whole genome shotgun sequence.
ACCESSION   DASUGJ010000496 DASUGJ010000000
VERSION     DASUGJ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355968"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2114)
                     /locus_tag="VFV93_11155"
     CDS             complement(<1..>2114)
                     /locus_tag="VFV93_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5165944.1"
                     /translation="EIVNTFVEVQDSVRRLMDDTDSLIRSGAWLSAQLPAAQPPAGVA
                     APGASYLPAHRWQLHRASELVALNDIIRTIASTRDLPRMYDLLYRKLSDLIDVDAMMV
                     LLASDPQTGTRQPPEMRRASFFPVIGNPAEAGVVEPVARVKRPLWIDDYQEFARERAI
                     ELPPVDDVQIPVPRSVIAAPIVVDDRFLGLLTVQALHPNAYDKRHVGLVEDIALHIGI
                     ALRNGSDVSWMFEQTQQGMVTRELQAELETAPSAEAAAQILAQRLRATVAYDGCIVFL
                     LEQGELTAAAVEGYYSLPERRAYQTYRMPRGVGIIWSSVEEGVSIVVPDLAGDSRGAV
                     LLRPPVAGESALVAPLTANGTTLGVVFLTRLSGTFDHHDEHRLTSLSLATARVLTGFQ
                     RRDQEQQRVRELAGLQRVISRLDAEITPEGTFDTFVRVLGEQFGYRLISIYLRDGDGL
                     TLQSQIGYDSVIDRIPIDKGVLARSIRTGTSILIEDVARDTEFLRAMPGITSEIAVPI
                     VVGGQIIGGLNIESGRERQLGGTDLPLIELLAQQLGMALGRMQRPASGHQSQPLVMTE
                     MIDQTTSLATQAAMMANLTEDVAEFHQTGAPLALLFIDLDHFKIANDAYGHRFGDELM
                     SWLGDFLPRTLPEDARIARYAGDAFVVTLPGTTIDTACTIAESLRAAMAAHTFETSLG
                     HSVLLSISVGVAGMERGGTLFE"
BASE COUNT          344 a          665 c          742 g          363 t
ORIGIN      
        1 tctcgaagag ggtgccgcct cgctccatac cggcgacgcc gacgctgatc gagagcagca
       61 ccgagtgtcc cagcgatgtc tcgaaggtgt gggcggccat cgcagctcgc agactctcgg
      121 cgatcgtgca ggcggtgtcg attgttgtgc ctggaagtgt gacgacgaag gcgtcgccgg
      181 cgtagcgtgc gattcgcgcg tcctcgggga gcgtgcgcgg caggaagtcg cccagccacg
      241 acatcagctc atcaccgaag cggtgaccgt aggcgtcgtt ggcgatcttg aagtggtcga
      301 gatcgatgaa cagtagcgcg agtggcgcac cggtctggtg gaactcggca acatcctcgg
      361 tcaggttcgc catcatcgcc gcctgggttg ccagggatgt cgtctggtcg atcatctccg
      421 tcatcacgag cggctgggac tggtggccgc tggccggtcg ctgcatcctg ccgagcgcca
      481 tgccgagctg ctgggcaagc agctcgatca acggcagatc ggtcccgccc agctggcgtt
      541 cgcggccaga ttcgatgttc agcccgccga tgatctggcc gccaaccacg attgggacgg
      601 cgatttccga ggtgatgccg ggcatggcgc gcagaaactc ggtgtcacgg gcgacatcct
      661 cgatcaggat cgacgttccg gtcctgatgc tgcgcgccag gacacccttg tcgatcggga
      721 ttcggtcgat gacgctgtcg tagccgatct gcgactgcag tgtcaggccg tcgccgtcgc
      781 gcaggtagat gctgatcagc cggtagccga actgctcgcc gagcactctg acgaatgtgt
      841 cgaaggtgcc ctcgggggtt atttcagcat ccagccggct gatgacgcgt tgcaggccgg
      901 ccagttcccg cacgcgctgc tgctcctgat cgcgccgctg aaagccggta aggaccctgg
      961 ccgttgccag cgacagcgac gtcagccggt gctcgtcgtg gtgatcgaac gtgccggaca
     1021 gccgcgtcag gaagacgacc ccaagcgtcg tgccgttcgc cgtcagcggg gcgaccagtg
     1081 ccgactcgcc ggcaaccggc gggcgcagca ggacggctcc ccggctgtct ccggccaggt
     1141 cgggcacgac gatcgacacg ccctcctcga ccgatgacca gatgatgccg acaccgcgtg
     1201 gcattcggta ggtctggtac gcgcgccgct cgggcaggct gtaatagccc tcgacggcgg
     1261 cggccgtcag ctcgccctgt tcgagcagga agacgatgca gccgtcgtag gcgacggtcg
     1321 cccgcaggcg ttgggcgagg atctgggcgg cagcttcggc agatggcgcg gtctccagtt
     1381 ctgcctgcag ttcccgcgtc accatgccct gctgtgtctg ctcgaacatc catgacacat
     1441 cgctgccatt gcgcagcgcg atgccgatat ggagcgcgat atcctcaacg agtccgacgt
     1501 ggcgcttgtc gtacgcattg gggtgcagtg cctgaaccgt cagcagcccg aggaaccggt
     1561 catcgacgac gatcggcgcg gcgatcaccg agcggggaac cgggatctgg acatcatcga
     1621 ccggtggcaa ctcgatcgcc cgctcgcggg cgaattcctg gtaatcgtcg atccagagcg
     1681 ggcgcttgac gcgggcaacc ggttcgacga cgccggcctc ggccggattg ccgatgaccg
     1741 ggaagaagga tgcacgccgc atttccggcg gctggcgggt gccggtctgc ggatcgctgg
     1801 ccagcaggac catcatcgcg tcgacgtcga tcagatcgct gagcttgcga tagagcaggt
     1861 cgtacatgcg gggcaggtcg cgcgtcgagg cgatcgtgcg gatgatgtcg ttgagggcga
     1921 ccagctcgct ggcgcgatgc agttgccagc ggtgggccgg caggtagctg gcgccgggcg
     1981 cggcgacgcc ggccggcggc tgggcggccg gaagctgcgc gctcaaccag gcgccgctgc
     2041 gaatcagcga gtcggtgtcg tccatcagtc gccggacgga gtcctggacc tcgacgaagg
     2101 tgttgacgat ctcc
//
LOCUS       DASUGJ010000497         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1607065, whole genome shotgun sequence.
ACCESSION   DASUGJ010000497 DASUGJ010000000
VERSION     DASUGJ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607065"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFV93_11160"
     CDS             <1..429
                     /locus_tag="VFV93_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165945.1"
                     /translation="RLATGMLTGTALAVFIWMMIAQIGFNGRARVRRSPLNGVGDLGL
                     LLAVEAVIAGIILTGWGPLRLPLTFVLLFSAIAVVTGLALAFVLLLGQREGKALVTAD
                     LAGPATVALVVALLLMGALGGGRFLLEWWLGIEPIVRSVA"
     gene            426..896
                     /locus_tag="VFV93_11165"
     CDS             426..896
                     /locus_tag="VFV93_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165946.1"
                     /translation="MSEPGVDSGSRSHGNGSDEWPSTPDGLVYQLDAVQALFGLVQQG
                     EQVDILDALLECVDWAEMFGSTGSGLLQREQIDELKRHYRARFESLDPFYLAEQLSTE
                     LMTALMASGDMVFSDALKQLGRDNPALWREIRTFFSRKEVATAMLMVAARPDAG"
     gene            906..1499
                     /locus_tag="VFV93_11170"
     CDS             906..1499
                     /locus_tag="VFV93_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165947.1"
                     /translation="MYRHRLRSPLRLLSATMSALMVVALAAIAGPWLIVLAPVVAGLF
                     LLAPPIGIYRHDETPAPRTGVRIEAVPLDDDSANTLRFRLVLVNPGDVPASNFRIRLL
                     VPHALVPASASSRLLGPIQAGAIGQNWFIDSTYDAIAVTFRSGKTDGITCPPAGRLDL
                     ADLNLPSQAHPLDVTLDYQISGGTAAPALDRLHLRWQ"
     gene            complement(1715..1906)
                     /gene="rpsU"
                     /locus_tag="VFV93_11175"
     CDS             complement(1715..1906)
                     /gene="rpsU"
                     /locus_tag="VFV93_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX5165948.1"
                     /translation="MKVELRESESFEGLLRRFTKEMQKSGKLREFRSKRRFVSKSEQR
                     RAKIRKAEHKRRRKEATGR"
     gene            2112..2708
                     /locus_tag="VFV93_11180"
     CDS             2112..2708
                     /locus_tag="VFV93_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165949.1"
                     /translation="MSERVGVIYCGSCGAMNPRTNHFCSACGHQLVDAYHASEGLRVY
                     ATPDPGAPLVEILDSGTNITVVDTDEELPADFVKIALDDGRIGYVRLREVEAAIGSDT
                     TPTRREPIGCITPTALIAVMALLILTAILMLLIAVRANDEGAIYPAMACLLIVPFLIF
                     VVGFYLYVRKREEELLAGDDEDDEQPAPSQQSPEPLSE"
BASE COUNT          423 a          943 c          893 g          496 t
ORIGIN      
        1 cggctggcga ccgggatgct gaccgggacg gcgctggccg tcttcatctg gatgatgatc
       61 gcccagatcg gcttcaacgg gcgggcacgc gttcgccgca gcccgctcaa cggcgtcggt
      121 gacctcgggc tgctgctggc cgtcgaggcc gtcatcgccg gcatcatcct gaccggctgg
      181 gggccgttgc ggctgccgtt gacgttcgtg ttgctgttct cggcgattgc ggtcgtgacc
      241 gggctggcgc tggcgttcgt cctgctgctc ggccagcgtg agggcaaggc gctcgtgacg
      301 gccgacctgg ccgggccggc gacggtcgcg ctggtggtgg cgctgctgct gatgggcgcg
      361 ctcggcggcg gacgctttct gctggagtgg tggctcggga tcgagccgat cgtgaggagt
      421 gtcgcgtgag tgagccgggc gtcgattcag gcagccgttc ccacggcaac gggagcgacg
      481 aatggcccag cacaccggac gggctcgtct accagctgga cgccgttcag gccctcttcg
      541 gcctcgtcca gcagggcgag caggtcgaca tcctcgacgc gctgctcgaa tgcgtggact
      601 gggccgagat gttcggcagc accgggagtg gcctcctgca gcgcgagcag atcgacgagt
      661 tgaaacgcca ctaccgcgcc cgcttcgaaa gcctggatcc gttttacctg gccgagcagc
      721 tcagcaccga gctgatgacg gcgctgatgg ccagcggcga catggtcttc tccgacgcac
      781 tcaagcagct tggccgcgac aacccagccc tctggcgcga gatccgcacc ttcttcagcc
      841 gcaaggaggt cgcgacggcg atgctgatgg tcgccgcccg gccggacgcc ggctagcggc
      901 cccgcgtgta ccgtcaccgg ctgcgctccc cgctgcggct gctctcggca acgatgagcg
      961 cgctgatggt cgtcgcgctg gccgctatcg ccgggccgtg gctgatcgtc ctggcgccgg
     1021 tcgtcgccgg gctgttcctg ctggccccgc caatcggcat ctaccggcac gacgaaacgc
     1081 cggccccgcg caccggcgtc cgaatcgagg ccgtcccgct cgatgatgat tccgctaaca
     1141 cgctccgctt ccggctcgtg ctggtcaatc ccggtgacgt cccggccagc aacttccgga
     1201 ttcggctgct ggtgccacac gcgctcgtcc cggccagcgc gtcgtcgcgg ctgctcggtc
     1261 caatccaggc cggcgcgatc ggccagaact ggttcatcga ctcgacctac gacgcgatcg
     1321 ccgtcacctt ccgctccggc aagaccgacg ggatcacctg cccacccgcc ggccggctcg
     1381 atctggccga tctcaacctg cccagccagg cccacccgct cgacgtcacg ctcgactacc
     1441 agatcagcgg cggcaccgca gcgccggcgc tggacaggct gcacctgcga tggcagtaat
     1501 ttgcagtttc caatctcaat ccggtacggg cgatatcgcg atatcgcccc gtatccccaa
     1561 ccggcgcgct caggtgggac acgatgggcg atattgcgat atcgcccgta ccggattgag
     1621 gcattgaatt ggatgcgggc ggctgccgat aacacgacag gacgcgaggt tgcccccgcg
     1681 tcccggtcat cgtcgtggat tgtcggaggc gggcctagcg gccggtggcc tccttgcggc
     1741 ggcgcttgtg ctctgccttg cggatcttgg cgcggcgctg ctcgctcttg ctgacgaagc
     1801 ggcgcttgct gcggaactca cgcagcttgc cggacttctg catctccttc gtgaacctgc
     1861 gcagcaggcc ctcgaaactc tcgctctcgc gaagctcgac cttcatgccg taaccacccc
     1921 ccttcgtctg ggattcgtgc aggtgcttct catcatcagt ccgacgacag gctgattcat
     1981 acgcacgcaa agacgactgt ctctccggac ccgccaattg taccgcactc ccgaacgctc
     2041 ctgctgatgg tgcgcctcgg ttggatatac tcggaaacgg ggctggcgcg ggctgagaga
     2101 acgggtggca ggtgtccgaa cgggttggcg tgatctattg cgggtcatgc ggggcgatga
     2161 atccgcgtac gaaccacttc tgctcggcct gcggacatca gctcgtcgac gcctaccacg
     2221 cctccgaagg gctgcgcgtc tacgcgacgc ccgatcccgg cgcaccgctg gttgagatcc
     2281 tcgactccgg cacgaacatc actgtcgtcg ataccgacga agagctgccc gccgatttcg
     2341 tcaagatcgc gctcgacgac ggccgcatcg gctacgtccg gttgcgcgaa gtcgaagcgg
     2401 ccatcggcag cgacacgacg ccgacgcgac gtgagccgat cggctgcatc acgccgacgg
     2461 cgctgatcgc cgtcatggcg ctgctgattc tgactgccat cctgatgctg ctgattgccg
     2521 tgcgcgcgaa cgatgagggc gcgatctatc cggcgatggc ctgcctgctg atcgtgccgt
     2581 tcctgatctt cgtcgtcggc ttctacctct acgtccgcaa gcgcgaagag gaactgctgg
     2641 ccggcgacga cgaggacgac gagcaacctg ccccttcgca acagtcaccc gaaccgctga
     2701 gcgagtaaat catggagcag atcgtcacga tcgaaggcat ccgccgctac acgat
//
LOCUS       DASUGJ010000498         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_77406, whole genome shotgun sequence.
ACCESSION   DASUGJ010000498 DASUGJ010000000
VERSION     DASUGJ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77406"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFV93_11185"
     CDS             <1..734
                     /locus_tag="VFV93_11185"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX5165950.1"
                     /translation="TMAEIEAANAAFKIAFVLAMEGGEPVQDGIESLRVFYELGVRIF
                     GFTWNQRNLLAEGVGEERAGGGLTELGREMVAEANRLGILLDVSHLSVKSFWDMIEAS
                     DAPIIASHSNAKALCGHRRNLDDDQIRALADKGGVIGINGVAAFISDDPKQASLERML
                     DHLDYIANLAGVEQVALGPDFVDFLLVGPGAQLHEPIDYPKGFENITTMPAVTTALLD
                     RGYTEDQVRGILGGNMLRLLGTVMG"
     gene            complement(960..2300)
                     /locus_tag="VFV93_11190"
     CDS             complement(960..2300)
                     /locus_tag="VFV93_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX5165951.1"
                     /translation="MGTFKLSGMTRTRVVQMITLLLAVVLLLVAFTRNNAGAVAPDGE
                     PFQRTWARTDKPVADLAVSRTWMWGPEANTIGEFEPYAEGHNGGRFVQYYDKSRMEIT
                     TDESVPYESPWFVTNGLLVNELMTGNLQLGDNLFEPHIPASINVAGDLDDTSGPTYAA
                     LAGLRDADTYPAGTVIDAVVDRFGNISSNPTFAAQNVTAAEYVPQTDHTVASIFWDFM
                     NSQGLVWQDGQYVTDDLFENAFYATGYPVIAAYWTTVSLAGVQTDVLLQCFERRCLTY
                     TPGNPDGWKVEAGNVGYHYHQWRYEQIPSEMTPTPTATTTATTPTATATTPTATSSVE
                     PSATLPEGVTGTGDMEIEGITSAGIIIRNMQIAGDIQMAGWQLRDDGGHVFTFPDVVV
                     REGFYITVRNGTGANIIENKYAFVYWGAYLEPYPEGRVYLYDSTGTLVDSFPDQ"
     gene            complement(2464..>2874)
                     /locus_tag="VFV93_11195"
     CDS             complement(2464..>2874)
                     /locus_tag="VFV93_11195"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEX5165952.1"
                     /translation="PAINRVVDLYNAVSIRHVLPVGGEDLDQLSSDLTLRYATGDEPF
                     SLISAGEEVIEHPEPGEVVWADSSGVTCRRWNWRQCRRTQLTEATRNAYFVLDAVAPY
                     SLDQLSAASDELTRLLEEASPGVTIERELLARPA"
BASE COUNT          463 a          878 c         1015 g          518 t
ORIGIN      
        1 agacgatggc ggagatcgaa gccgccaacg cggccttcaa gatcgcgttc gtgctggcga
       61 tggagggcgg cgagccggtc caggacggga tcgagtcact gcgcgtcttc tacgagcttg
      121 gcgtgcggat cttcggcttc acctggaacc agcgcaacct gctggccgag ggggtcggcg
      181 aggagcgggc tggtggcgga ttgaccgagt tggggcgcga gatggttgcc gaggccaacc
      241 ggctcggtat cctgctggat gtctcgcatc tctcggtcaa gtcgttctgg gacatgatcg
      301 aggcgtcgga cgcgccgatc atcgcctcgc actcgaacgc aaaggcgctc tgcgggcatc
      361 gccgcaacct ggacgacgac cagatccgcg cgctggccga caagggcggc gtgatcggca
      421 tcaacggcgt cgcggccttc atcagcgacg atccgaagca ggccagcctg gagcggatgc
      481 tggaccacct ggactacatc gccaacctgg ccggtgtcga acaggtcgcg ctcggccccg
      541 acttcgtcga tttcctgctg gttggccccg gcgcgcagtt gcatgaaccg atcgactacc
      601 cgaagggttt cgagaacatc acgacgatgc cggccgtcac caccgcgctg ctggatcgcg
      661 gctatacgga ggaccaggta cgcggcatcc tcggcgggaa catgctgcgg ctgcttggga
      721 cggtgatggg gtagacgcag cggtgccgcc gtttcgttgt gtaggggccg atcctgtatc
      781 ggaccgcgtc gtgaggcgcc tacgttggtt gtgggtgtca aacgaggatc cctaacgcgt
      841 gcgcccgtgt gggaaccgtg cctgtgccaa gcgtgcgcct cacggcgcgg gccggtacag
      901 agacacggcc cctacacggg gaaggcggcg gcacgtcctc ccatagccca gcctcagcgc
      961 tactgatccg ggaacgaatc cacgagcgtg ccggtgctgt cgtagaggta gacgcggccc
     1021 tcggggtatg gctcgaggta ggcgccccag tagacgaagg cgtatttgtt ctcgatgatg
     1081 tttgcgccgg tgccgtttcg gacggtgatg tagaagcctt cgcggacgac gacatccggg
     1141 aaggtgaaga cgtggccgcc gtcgtcgcgt agctgccagc cagccatctg gatgtcgccg
     1201 gcgatctgca tgttgcggat gatgatgccg gcgctggtga tgccctcgat ttccatgtcg
     1261 ccggtccccg tcacgccttc cggcagggtc gcgctcggct cgaccgacga cgtggccgtc
     1321 ggggtcgttg cggtcgcagt cggcgttgtc gcggtcgttg tcgcggttgg agtcggtgtc
     1381 atctccgacg ggatctgctc gtagcgccac tggtggtagt ggtagccgac attgcctgcc
     1441 tcgaccttcc agccgtccgg gttgcccggc gtgtaggtca ggcagcggcg ctcgaagcat
     1501 tgcagcagca cgtcggtctg gacaccagcc agcgagacgg tcgtccagta ggccgcgatc
     1561 accgggtagc cggtcgcgta gaaggcgttc tcgaacagat cgtcggtcac gtactggccg
     1621 tcctgccaga cgagcccctg cgagttcatg aagtcccaga agatggaagc gaccgtgtgg
     1681 tcggtctgcg ggacgtattc ggcggcggtg acgttctggg ccgcgaacgt gggattgctg
     1741 gagatgtttc cgaagcggtc gacaacggcg tcgatcaccg tgccggccgg atatgtgtcg
     1801 gcgtcacgca agccggccag agcggcgtag gtcgggccgc tggtgtcgtc aaggtcgccg
     1861 gcgacgttga tcgaggcggg gatgtgtggc tcgaagaggt tgtcgccgag ctggagattg
     1921 ccggtcatca gctcgttgac gagcaggccg ttagtcacga accacgggct ctcgtacggg
     1981 acgctctcgt cggtcgtgat ctccatccgc gacttgtcgt agtactggac gaagcgcccg
     2041 ccgttgtggc cctcggcgta cggctcgaat tcgccaatcg tgttggcctc cggcccccac
     2101 atccaggtac gcgagacggc caggtcggcg accggcttgt cggttcgcgc ccaggtgcgc
     2161 tggaacggct cgccatcggg cgcaaccgcg ccggcattgt tgcgggtgaa tgcgaccagc
     2221 agcagcacga cggccagcag cagcgttatc atctggacga cgcgggttcg cgtcatcccc
     2281 gacagtttga aagttcccat ctgccccccg aatcaacgac acgcctgcgc ccgatcgcga
     2341 ccggcgcgcc cggcactccg acactacccc acgcgccaat cgtttcatat gacgctaacg
     2401 accgcacaga tgacgcggat gtcgcgagtc ggcgacagcg cggcgtggga gccggccagg
     2461 ccgtcaggca ggtcgggcga gcagttcccg ctcgatcgtg acgcctggtg atgcttcttc
     2521 gagtaagcgg gtcagttcgt cactcgcggc cgagagctgg tcgagtgaat agggcgcgac
     2581 ggcatcgagg acgaagtagg cgttgcgggt ggcttcggtc aactgggtgc ggcggcactg
     2641 gcgccagttc caacggcggc aggtcacgcc gctgctgtcg gcccagacga cctcgcccgg
     2701 ttcggggtgt tcgatgacct cctcaccggc gctgatgagg gagaacggct cgtcgccggt
     2761 cgcgtagcgg agggtcaggt cgctgctgag ctgatcgagg tcctcgccgc cgaccggcag
     2821 gacatgccgg atgctgaccg cgttgtagag atcgacaacc cggttgattg ccgg
//
LOCUS       DASUGJ010000499         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1745995, whole genome shotgun sequence.
ACCESSION   DASUGJ010000499 DASUGJ010000000
VERSION     DASUGJ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745995"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFV93_11200"
     CDS             complement(<1..619)
                     /locus_tag="VFV93_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5165953.1"
                     /translation="MNARFVLLAAFVIGTALVNWPKDTYSWSPSPASLQPRFEAASCP
                     FVLGPDQIEGQTVTCGFVTVPEMRGAHAGQTIRLAVAVFKAQDTASSPPLIFLGGGPG
                     SAILGSFGPVITESLAEGLTADHDLILFDQRGAGFSQPSLACQEMTDLKYQNLGLHIS
                     REDETDGTVQAALRCRDRLRQAGINLAAYTTAASVADVDDIRRALG"
     gene            complement(999..>2395)
                     /locus_tag="VFV93_11205"
     CDS             complement(999..>2395)
                     /locus_tag="VFV93_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5165954.1"
                     /translation="DDTSAQRINRFVDGHPLALQLAAANAHEASRLDFADVAVQRVIE
                     ELARTYLADVPDPLTRRTLEAASVVRRITLPLLRAILPDIAPQDGYDRLRALPFVETS
                     RDGLHIHDVIQHALAASLHAADPERHRALRSAAWHRLRADVRLAAPTDLWRYTADMLY
                     LIENPIIREGFFPKDSHRFAFEPALDVDGSAIREIITTTEGPESAQLLLQLWERAPQI
                     FRAAHDHDGSIAGFYVMFDPADVDAQVMRDDPILQHWLQHLAASPMPEGQTSLFIRRW
                     LGRGTGELPSPVQGATWLDLKRAYMELRPRLRRVYMTIWAMDTYAATAQELGFVPLPE
                     AHVTLDQKTYYSALNDLGPQSVDGWLARLVGAELGITLEDGILDIDARELVLDGVRVP
                     LTRLEFETLHYLYVRENKAVSRETLLDEVWGDSYAGGSNVVDVIVRSLRKKLGDRAAT
                     VETIWGTGYRYRQP"
BASE COUNT          428 a          715 c          814 g          438 t
ORIGIN      
        1 agccgagcgc gcggcggatg tcatcaacgt cggcgacgct ggcggccgtg gtgtacgcgg
       61 cgaggtttat acccgcctgc cggagtcggt cccggcagcg cagcgcggcc tggaccgtgc
      121 cgtcggtctc gtcctcccgg ctgatgtgca gcccgaggtt ttgatacttc aggtccgtca
      181 tctcctgaca cgcgagggac ggttgtgaga agcctgcacc acgctggtcg aacaggatca
      241 gatcgtggtc ggcggtgagg ccctcggcca gcgactcggt gatgacgggg ccgaagctgc
      301 ccaggatagc ggatccgggg ccgccaccga ggaagatgag cggcggggag ctggcggtat
      361 cctgcgcctt gaatacggcg acggcgaggc ggatcgtctg gccggcatgt gcaccgcgca
      421 tctcgggtac cgtgacgaac ccgcaggtca ccgtttgtcc ctcgatctgg tcgggtccga
      481 gcacaaacgg acaggacgcg gcctcgaaac gaggctggag cgacgctggc gacggggacc
      541 aggagtaggt gtccttcggc cagttcacga gggcggtccc gatgacaaag gcggcgagga
      601 gcacgaatcg cgcgttcatg attggccccc tggggccgcc gactcgaagc cgagttcgac
      661 tcgccgcagt cgcgcgaatc tgcccatcgg cgatctttgc agcggagtag tacggccgac
      721 aaggtcggta cagcacgttc gaggcatgat gtgacctccg tccggtattg cctgccaagc
      781 ggatccgctc ttgtgcgttg gggacgcata gatgcgaccg ccctatccat cacgctggca
      841 gtatgggctg cgcgctgaga gtacgcatcg cgcagcttcg tcattcagga accgacgggg
      901 catgacgatt cttcgttatt ggttggagaa gcgcttgccg attccgtggc gcactacccc
      961 ggcagccacc gctgcgtgtg acctggtgga atcctgaatc agggctggcg gtagcgatag
     1021 ccggtgcccc agatggtttc tacggtggct gcacggtcgc cgagcttctt cctcagtgac
     1081 cggacgatga catcgacgac gttgctgccg cctgcgtaag agtcacccca gacttcgtcg
     1141 agcaacgtct cacggctgac ggctttgttc tcgcgaacgt agagatagtg cagcgtctca
     1201 aactcaagtc gtgtcagggg cacgcgaacg ccatccagta ccagctcgcg agcgtcgatg
     1261 tcgaggattc cgtcttcgag tgtgataccc agttcggcac cgacgagccg cgccagccag
     1321 ccgtccaccg attgcggccc caggtcgttg agcgctgagt agtaggtctt ctggtcgagc
     1381 gtgacatgtg cctcgggcag cggcacaaaa ccaagttcct gcgccgtcgc cgcgtaggta
     1441 tccatcgccc agattgtcat atagacgcga cgcaatcgag gacgcaactc catgtacgcg
     1501 cgcttgagat cgagccaggt tgcgccctgg accggagacg gtaactcgcc tgttccacgg
     1561 ccgagccagc gccgaatgaa taacgaggtc tgtccttccg gcatcggcga tgcagccagg
     1621 tgctgcagcc agtgctgaag aatcgggtcg tcgcgcatga cctgtgcgtc aacgtcggcg
     1681 gggtcgaaca tgacgtagaa accagcgatc gagccgtcgt gatcgtgggc ggcgcgaaag
     1741 atctgtggcg ctcgctccca gagctgcagc agcagctgag cgctctcagg gccttcggtt
     1801 gtcgtgatga tctcgcggat ggctgatcca tcgacatcca gcgccggttc gaacgcgaag
     1861 cgatggctgt ccttcgggaa gaagccctcg cgtatgatcg ggttctcgat caggtacaac
     1921 atgtcggctg tgtaacgcca gaggtcggtt ggcgcggcca ggcgaacatc ggcgcgcagg
     1981 cggtgccagg ccgcgctgcg taacgctcgg tgccgttctg gatcggctgc gtgcaggctg
     2041 gctgccagcg cgtgctggat cacgtcatgg atgtgcagcc cgtcgcggct ggtttcgaca
     2101 aagggaagcg cgcgcaggcg atcgtagcca tcttgaggcg ctatgtcggg cagaatggcg
     2161 cgcagcagcg gcaacgtgat gcggcgtacg accgaggccg cttcaagcgt ccgtcgggtc
     2221 agcggatcgg gaacgtcggc cagatacgtg cgtgccagtt cttcgatgac ccgttggacg
     2281 gcaacgtccg cgaaatccag ccgcgaagcc tcatgcgcgt ttgctgccgc cagttgcaac
     2341 gcgagtggat ggccatcgac gaaacggttg atacgctggg cgctggtgtc atcga
//
LOCUS       DASUGJ010000500         7544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_912004, whole genome shotgun sequence.
ACCESSION   DASUGJ010000500 DASUGJ010000000
VERSION     DASUGJ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912004"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFV93_11210"
     CDS             <1..257
                     /locus_tag="VFV93_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165955.1"
                     /translation="NDPLIIPFWNETQRQLDTTRLLTTFRRVGVPNVWWVSAKALIEV
                     IQQRLVQQLGWLAESDPSAANTLAEAAEDIFQRAQALTGR"
     gene            396..758
                     /locus_tag="VFV93_11215"
     CDS             396..758
                     /locus_tag="VFV93_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5165956.1"
                     /translation="MSRRILVVDDSATEMAVMSSSLKAEGFDVLTAANGDEALTHLEQ
                     TTPDAIVLDVIMPGRNGFQLCRQIRNDERWSKVPIVLVTSKDQESDRFWGMKQGASDY
                     ITKPFAPASLVAAVRRFV"
     gene            778..1317
                     /locus_tag="VFV93_11220"
     CDS             778..1317
                     /locus_tag="VFV93_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5165957.1"
                     /translation="MAHLAEIELAPAVVEREPTWIGEQYIVLTIAGTDIAVPLSMVLE
                     VERVPALVPVPGGQGWVRGIANLRGTILTVVDLAAMLDLGSWTQRHDARVIVVRSAEP
                     LALGVDAVRGMRRLAPGSRFQIDGALPEHLTRYVTGIHRDGGDLIHTIDLPRLLDDAD
                     ESARRSGAERITGLLSDRL"
     gene            1371..3590
                     /locus_tag="VFV93_11225"
     CDS             1371..3590
                     /locus_tag="VFV93_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5165958.1"
                     /translation="MEDRRTQILERAREGLRIGDWSVGRKIITLVAVLTLVPLIAMTT
                     MTYVQSSRALNNEIDQSLEQQALTSAEALDNAINKAAQDTLLLTTNDFLFSQSVGIGT
                     KQDFLNAHDIVWGYGDLAVFNNDGKLFAGTGENYTDQKTEDYWDKVVNLPDGQVYFSD
                     IAVDAGSGKMAVQVAAPAYNEAGILRGVVRMLWTADSLQAVMNGLGIESDVQIDLINT
                     RHAVVASTLGAQIGRVYPESEAAEQALAGQQDTVSESIVATIDGVDVTKDVITGYAPI
                     RGNERLAGLGWGVLVHKDADTAFAPIATIRNFAIVLLLVVGVLALAAAWYFGRKLVEP
                     IRNLADVAGRVREGDMTARATVQTQDEVGQTAASVNQMLDEITALVQTKEERDNLQRQ
                     ITGLLMEVSDVAEGDLTVEAQVTADTLGSVADAFNYMIGELRQIISNVNETTVAVSSS
                     SADILQTSASLARASETQAQQIADTSLAVNEMSRSIQQVSQNATVSAEVAREARNNAQ
                     AGSKAVHATIEGMQRIRSEVQETSRTIKRLGESSQEIGQIVQLIEEIANQTNLLALNA
                     AIQAAMAGEHGRGFAVVAEEVRRLAERAAGATRQINTLVTSIQTETAEAVVSMDNSTR
                     EVVAGSRLADEAGGALVQIDGVVGRLSELIDAISDAAEQQAQASVEITQAMTNISEIT
                     QTTSTGTLQAAESVNHLASLAERLRQSVATFKLTRDNKPQPAQPDWNYGVGIAADGD"
     gene            3706..>7544
                     /locus_tag="VFV93_11230"
     CDS             3706..>7544
                     /locus_tag="VFV93_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX5165959.1"
                     /translation="MAKHGESELIENFITELQPYLALIDQAVEELARLAPDTQVVGQA
                     AESLAIIRDASTVLNFEGLVRLTSALQDTFDDLLDSVTPDPDRLLDQARAMAIGIHRY
                     MDALEDGNDTDELVTSVLAPFDRPAGPEPEDAFFGAAPAAGTILLDLDDLPVAEADPE
                     LREIFEEEATELIASFTRGALMLVSAPDRPALLSGLRRDAHTLKGAANMTGFPLIGSL
                     GASIETLLDAHIDGDAPVERETLGVILSARKLLTGMLASLDDTTGFAEQSRALNERAA
                     AITARIAPLAQATDEPDEDELLDLPAVTPEDILTSELVLEPESEPAREAIDELLVEEP
                     ADTIGAEEAEASDETVEAAAVADAEDAVVESVETEPVVDLDVEKPVEEAVELAQPEPE
                     PAPLASEPTMASDLIPFTYDAIEPEAADVVLDEGLGDEVAWVWDVLPDETRLETESAE
                     PGIDASEPAANEPEQIDEHDPAGSLFGQVDNVEAPDEEPELDVYEMAAEIAMLARLRT
                     SLLPQNATFFPTDASGLSDDIDALTAEIVDTTAGGLPLIESVEDEDAGEEITPVATAE
                     PELADPIATIDAEPEPSLAELADTGTSLGYALLDLAFEDAGAPETDWPDISAADTAAS
                     DERMQQELRETFAVEAAEYADSLTLAAMALERDPESSDALRELTRVLHTLKGAAAAAG
                     YDEIGEHCHAIEDDLAAGADITRAFTDRVLELASTIEQATTGATTPRYQPDDDATAGP
                     GPAQPAARTAGDLRVEMRRLDDLLNLVGELVINRSTLDQRLLRLSASLDELSHMTERL
                     RRSGQTLERVAGEASLFGPLPAAGDDRIIPFVGHDLRHEFDTLEMDRYTDLDVLAREL
                     GEVASDISAGAGEVRNLRGDFETVSTHQRRLTNEIQDDLMDIRMVPLATLSPLLHRVV
                     RRVAQECDKDVDLVIEGSEIAFDKALVDALGDALLHLLRNAVDHGIESPAVRWTTGKP
                     ERGRIVVRAWQDRGNAVVEVIDDGRGVDTERLVERARNFGFISAETVSEAEALQLMFL
                     PGFSTRDSASSVSGRGVGLDAVREAVERVKGRVTVDSKPGAGTIFQLRLPVMLAVAQA
                     FLVEAGGRRYAVPLADVDFVADRRQARLSRVGNAALVEINEALIPAIDLASRLAGDSD
                     RLDEETGWLLVTRTGGDPRAMRVDSLHGQQEIVVKPLGRYLTAAPGVTGATILGDGQI
                     ALIIDVADVTGKRTLAEPAGQTAEPTPIDSGHHGVGTALIVDDSLSVRRVLARTLERH
                     GWTTIQAHDGVEALEKV"
BASE COUNT         1374 a         2630 c         2472 g         1068 t
ORIGIN      
        1 ccaacgaccc actgatcatc ccattctgga acgagaccca gcgccagctc gacacgaccc
       61 gactgctgac gaccttccgt cgcgtcggcg tcccgaacgt ctggtgggtc tccgccaagg
      121 cgctgatcga ggtgatccag caacggctgg tccagcagct cggctggctg gccgagagtg
      181 atccgagcgc cgccaacacg ctggccgagg cggccgaaga catcttccag cgggcgcaag
      241 ccctgacggg gaggtgagac aacggcagca gaccagcaac gcccatcgaa cgaattgttg
      301 tcgggattcg ggacgcggca ttcggggatg ttccaccgac cgtgctccaa atcccggttc
      361 ccgaatcccg aatccctatt tagaaggagt accccatgag ccgaagaatt ctcgtcgtcg
      421 acgatagcgc aactgagatg gccgtgatgt ccagctcgtt gaaggccgag ggtttcgatg
      481 tactgacggc cgcgaacggc gatgaggcgc tgacccacct cgaacagacg acccccgacg
      541 cgatcgtcct ggacgtcatc atgccgggcc gcaacggctt ccagctctgc cgccagattc
      601 gcaacgacga gcgctggtcg aaggtgccga ttgtcctggt cacctcgaag gaccaggagt
      661 cggaccgctt ctggggcatg aagcagggcg ccagcgacta catcacgaag ccgttcgcgc
      721 cggccagcct cgtcgcggcc gtccggcgct tcgtctagca cgcgggaagg cagagagatg
      781 gcgcatctgg cagagattga gctggcgccg gcggttgtcg agcgcgaacc aacctggatc
      841 ggcgagcagt acatcgtcct gacgattgcc gggaccgata tcgccgtccc gctctcgatg
      901 gtgctggagg tcgagcgcgt gccagcgctc gtgccggttc cgggcgggca gggctgggtg
      961 cgtgggatcg ccaacctgcg cggcacgatc ctgaccgtcg tcgatctggc cgcgatgctc
     1021 gacctcggca gctggaccca gcgccatgac gcccgcgtga tcgtcgtccg ctcggccgaa
     1081 ccgctggcac tcggcgtcga tgccgtccgt ggcatgcgcc ggctcgcgcc cggctcgcgc
     1141 tttcagatcg atggcgcgct gcccgagcac cttactcgtt acgtcaccgg catccaccgt
     1201 gatggcggcg acctgatcca taccatcgac ctgccgcgac tactggacga cgccgacgag
     1261 agcgcacgcc gctccggcgc cgaacggatt accggcctgc tcagcgaccg tctctagcaa
     1321 tccggccgga ccacgcccgg agcatgctgg cgaacagaag ggggactctg gtggaagatc
     1381 gacggacaca gattcttgag cgcgcgcgcg aagggctgcg catcggcgac tggagcgtcg
     1441 ggcgcaagat catcacgctc gttgccgtcc tgacgctcgt gccgctcatc gcgatgacga
     1501 cgatgaccta tgtgcagtca tcgcgcgcac tcaacaacga gatcgaccag tcgctggagc
     1561 agcaggcgct cacctcggcc gaggcgctcg acaacgcgat caacaaggcc gcccaggaca
     1621 cgctgctgct gacaaccaac gacttcctct tctcccagtc ggtcggtatc ggcaccaagc
     1681 aggacttcct gaacgcgcac gacatcgtct ggggttacgg cgacctggcc gtcttcaaca
     1741 acgatggcaa gctcttcgct ggaacgggcg agaactacac cgatcagaaa accgaagact
     1801 actgggacaa agtcgtcaac ctgccggatg gccaggtcta tttctccgac atcgccgttg
     1861 acgccggatc gggcaagatg gccgtccagg tcgctgcgcc cgcctacaac gaagccggca
     1921 tcctgcgcgg tgtcgtccgt atgctctgga cggccgatag cctccaggcc gttatgaatg
     1981 gcctcggcat cgagtccgac gtccagatcg acctgatcaa cacccgccac gccgtcgtcg
     2041 ccagcacgct cggcgcccag atcggccggg tctatcccga gtctgaagcg gctgagcagg
     2101 cactggccgg ccagcaggac accgtctccg agtcgatcgt cgcgacgatc gacggcgtcg
     2161 atgtcaccaa ggacgtcatc accggctacg cgccgatccg cgggaacgag cggctggccg
     2221 gtctcggctg gggcgtgctc gtccacaagg acgccgacac cgccttcgcg ccgatcgcaa
     2281 cgatccggaa cttcgcgatc gtcctgctgc tggtcgtcgg cgtcctcgcg ctggccgccg
     2341 cctggtactt cggccgcaag ctggtcgagc cgatccgcaa cctggccgac gtcgccggcc
     2401 gcgtccgcga gggtgacatg accgcccgtg cgaccgttca gacccaggac gaagtcggtc
     2461 agacagccgc ctcggtcaac cagatgctcg acgagatcac ggcgctggtt cagacgaagg
     2521 aagagcgcga caacctccag cgccagatca ccggcctgct gatggaagtg tccgatgtcg
     2581 ccgaaggaga cctgaccgtt gaagcgcagg tgacggccga cacgctcggc tcggtcgctg
     2641 acgcgttcaa ctacatgatc ggcgagctgc ggcagattat ttccaacgtc aacgagacga
     2701 cggtcgccgt cagcagctcc tcggccgaca ttctgcagac ctcagcctcg ctggcccgcg
     2761 cctccgagac gcaggcccag cagatcgccg acacctcgct ggccgtgaac gagatgtccc
     2821 gctcgattca gcaggtgtcg cagaacgcaa ccgtctcggc cgaagtcgcc cgcgaagcgc
     2881 gcaacaacgc ccaggccggc tcgaaggccg tgcacgcgac gatcgagggc atgcagcgca
     2941 tccgctccga agtgcaggaa acgtcgcgga cgattaagcg actcggcgaa agctcgcagg
     3001 agatcggcca gatcgtccag ctgattgagg aaatcgccaa ccagacgaac ctgctggccc
     3061 tcaacgccgc catccaggcc gcaatggcgg gagagcatgg acgcggcttc gccgtcgtcg
     3121 ctgaggaagt ccggcggctg gccgagcgcg cggcgggtgc gacccgccag atcaatacgc
     3181 tcgtgaccag tattcagacg gagacggccg aagcggtcgt ctcgatggac aacagcaccc
     3241 gcgaggtcgt cgccggttcc cggctggcgg acgaggccgg tggcgcgctg gtccagatcg
     3301 acggcgttgt tggccggctc tccgagctga ttgacgccat ctccgacgcc gccgaacaac
     3361 aggcacaggc atcggtcgag atcacccagg cgatgacgaa catctccgag atcacccaga
     3421 cgacatcaac cggcacgctc caggcggccg aatcggtcaa ccacctggcc tcgctcgccg
     3481 agcggctgcg ccagtcggtc gcgaccttca agctcacccg cgacaacaag ccgcaaccgg
     3541 cccagccgga ctggaattac ggcgtcggca ttgcggcgga tggtgactag cgtcgggatt
     3601 ggggattcgg gatttggggc tggctaacaa cccgcccctt ccccgaatcc cgtgtcccga
     3661 atcccgaatc ccgatcgacg aacatgcgct aagggagcca cgttcatggc caaacacggc
     3721 gaaagcgaac tgatcgagaa cttcatcaca gagctccagc cgtatctggc gctgatcgat
     3781 caggcggtcg aggagctggc gcggctcgcg ccggacaccc aggttgtcgg tcaggcggcc
     3841 gagtcgctgg cgatcatccg tgacgccagc acggttctca acttcgaggg actggtgcgg
     3901 ctgacgagcg ccctgcagga cacgttcgac gacctgctcg actcggtgac gcccgatccc
     3961 gatcgcctgc tcgaccaggc cagggcgatg gctatcggca tccaccgcta catggacgcg
     4021 ctcgaagatg gcaacgacac cgacgagctg gtcaccagcg tcctggcgcc gttcgacaga
     4081 ccggcagggc cggagccgga ggacgcattc ttcggcgctg cgccggcagc cggaacgatc
     4141 ctgctcgatc ttgacgacct gccagtggcc gaggcagatc cggaactgcg tgagatcttt
     4201 gaagaggaag cgaccgaact gatcgcctcg ttcacccgtg gggcgctgat gctggtcagc
     4261 gcacccgacc ggcctgccct gctctccggc ctgcggcggg acgcccacac gctcaagggc
     4321 gccgccaaca tgaccggatt cccgctgatc ggctccctcg gcgcatcgat cgagacgctg
     4381 ctggacgccc acatcgacgg cgacgcgccg gtcgagcgtg agacgctggg ggtcatcctc
     4441 agcgcccgca agctgctgac cggcatgctg gccagcctcg acgacacgac aggcttcgcc
     4501 gagcaatccc gcgccctgaa cgagcgcgcc gccgcgatca ccgcccggat cgcgccgctc
     4561 gcccaggcga ccgacgagcc cgacgaggac gagctactcg acctgccggc agtcacgcct
     4621 gaggacatcc tcaccagcga actggtactg gagccggagt ccgagcctgc ccgcgaggcc
     4681 atcgacgaac tgctcgtcga agaaccggcc gacaccatcg gtgctgagga agcagaagcg
     4741 agcgacgaga ccgtcgaagc cgcagcggtg gcagatgcag aggacgcggt ggtcgaatcg
     4801 gtcgagaccg agccggttgt cgatctggac gtcgaaaagc cggtcgaaga agcagttgag
     4861 ttggcgcagc cagaaccgga gccggcaccg cttgccagcg agccaaccat ggcctccgat
     4921 ctgattccgt tcacctacga cgcgatcgag ccggaagccg ccgatgtcgt cctcgatgag
     4981 gggttgggcg acgaggttgc ctgggtctgg gacgtgctgc ccgacgagac caggctggag
     5041 accgagagcg ccgagccggg gatcgatgcg agcgagccgg ctgccaacga gccggagcag
     5101 attgacgaac acgacccggc cggcagcctg tttgggcagg tggacaacgt cgaagcgccg
     5161 gatgaagagc cggaactgga tgtctacgag atggcggccg agatcgcgat gctggcccgg
     5221 ctgcggacct cgctgctgcc gcagaacgcg accttcttcc cgaccgacgc cagcggcctc
     5281 tccgacgaca tcgacgcact gacggccgag atcgtcgaca cgaccgccgg tggcctgccg
     5341 ctgatcgaga gcgtcgagga cgaggacgcg ggagaggaaa tcactccggt tgccactgcc
     5401 gaaccggagc tagccgaccc gatcgcgacg atcgacgccg aaccggaacc ctcgttggcg
     5461 gagctggcgg acaccggtac gtcactcggc tacgcgctgc tcgacctcgc cttcgaggac
     5521 gccggcgcgc ccgagacgga ctggccggac atcagcgccg ccgacaccgc cgccagcgac
     5581 gagcgcatgc agcaggagct acgcgagacc ttcgcggtcg aggcggccga gtacgccgac
     5641 agcctgacgc tggcggcgat ggcgctcgaa cgcgacccgg agagcagcga cgccctgcgc
     5701 gagctgacgc gcgtgctgca taccctcaag ggcgcggcag ccgcggccgg ctacgacgag
     5761 atcggcgagc actgccacgc gatcgaagac gacctggcgg ccggcgccga catcacgcgc
     5821 gccttcaccg accgggtgct ggagcttgcg tcgacgatcg agcaggcaac gaccggcgcg
     5881 acgacgccac gctaccagcc cgacgacgac gcgaccgccg gccccggccc ggcccagccg
     5941 gcggcacgga cggccggtga cctgcgcgtc gagatgcgcc ggctggacga tctgctcaac
     6001 ctggtcggcg agctggtcat caaccgctcg acactcgacc agcgccttct gcgcctgagc
     6061 gcctcgctgg atgagctgtc gcacatgacc gagcgcctgc gccggtccgg ccaaacgctg
     6121 gaacgcgtcg ccggtgaggc cagcctgttc ggtccgctgc cggcagcggg tgacgaccgg
     6181 atcatcccgt tcgtcggcca cgacctgcgc cacgagttcg acacactcga gatggaccgc
     6241 tacaccgatc tggatgtcct cgcccgcgag ctgggcgagg tcgcctccga catcagcgcc
     6301 ggcgcgggtg aggtccgcaa cctgcgcggc gacttcgaga ccgtctccac ccaccagcgc
     6361 cggctgacga acgagatcca ggacgacctg atggacatcc gcatggtgcc gctggcgacg
     6421 ctctcgccgc tgctgcaccg cgtcgtccgc cgcgtcgccc aggagtgcga caaagacgtc
     6481 gacctcgtca tcgagggcag cgagatcgcc ttcgacaagg cgctggttga cgcgctcggc
     6541 gacgcgctgc tgcacctgct gcgcaacgcc gtcgaccacg gcatcgagtc gcccgccgtc
     6601 cgctggacga ccggcaagcc ggagcggggc cggatcgtcg tccgtgcctg gcaggatcgc
     6661 ggcaacgccg tcgtcgaggt catcgacgac gggcgcggcg tcgacaccga gcggctggtc
     6721 gagcgagcgc gcaacttcgg cttcatcagc gccgagaccg tcagcgaggc cgaggcactc
     6781 cagctcatgt tcctgccggg cttcagcacc cgcgacagcg ccagcagcgt gtcaggccgc
     6841 ggcgtcgggc tggacgccgt ccgcgaggcg gtcgagcgcg tcaagggccg cgtcaccgtc
     6901 gattccaagc cgggagccgg gacgatcttc cagctgcggc tgccggtcat gctggccgtc
     6961 gcccaggcgt tcctggtcga ggccggcgga cgccgctacg ccgtcccgct ggccgatgtc
     7021 gacttcgtcg cggaccgccg ccaggccagg ctgagccgcg tcggcaacgc cgccctggtc
     7081 gagatcaacg aggcgctgat ccccgccatc gacctcgcca gccggctggc cggcgacagc
     7141 gaccggctcg acgaggagac cggctggctg ctcgtcacgc ggaccggcgg cgatccgaga
     7201 gcgatgcggg tcgatagcct gcacggccag caggagatcg tcgtcaagcc gcttggccgc
     7261 tatctgacgg ctgcccccgg cgtgaccggc gcgacgatcc tcggcgacgg ccagatcgcc
     7321 ctcatcatcg atgtcgccga tgtgaccggc aagcgcacac tcgccgagcc ggccggccag
     7381 accgccgaac caaccccgat cgacagcggc caccacggcg tcggcaccgc cctgatcgtc
     7441 gacgactcgc tctcggtccg ccgcgtcctg gcccgcaccc tcgaacgcca cggctggacc
     7501 acgatccagg cccacgacgg cgtcgaggcg cttgagaagg tcca
//
LOCUS       DASUGJ010000501         3899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_356586, whole genome shotgun sequence.
ACCESSION   DASUGJ010000501 DASUGJ010000000
VERSION     DASUGJ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356586"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2256
                     /gene="gyrA"
                     /locus_tag="VFV93_11235"
     CDS             <1..2256
                     /gene="gyrA"
                     /locus_tag="VFV93_11235"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5165960.1"
                     /translation="GQGNFGSVDGDGAAAMRYTETKLSAIAEEMLIDIDKDTVDFKPN
                     YDDQTREPKVLPAKLPNLLVNGASGIAVGMATNIPPHNLTEVCNGIIHLIDHPEATVD
                     DLMQFITGPDFPTGATILGREGIMAAYATGRGRVVIRANAFIEEMARGNRYAIIVSEL
                     PYQVNKAALIEKIAELVKIGRLDGIHDLRDESDRNGMRIVIELKRDAQPRKVLNNLFK
                     HTALQTSFGVNALALVDGEQPRVLTLKRILQLHIEHRQEILTRRTQFELERAKRRAHI
                     LEGLKIALDNLDAIIKTIRASRTSESARTNLMKDFKLSEVQATAILDMQLRRLAALER
                     KKIEDEYREVLKEIARLEDLLANPRKILALIKDDLTYLKEKFGDARRTRIVEDPGEIS
                     DDDLIPEVDVIVTLTNRGYVKRIPDDVYKTQRRGGRGVTGLTMREADGVQHLLAANSH
                     DSLLFFTDRGRVFQVKVHELPDAGRTAKGVPIINLISILPNESIATMLPVKDFAESSY
                     LFFCTRKGRIKRTSLDQFSSVRSSGLIAIGLDDDDELAWVRTTSGDDEIILVTEQAKA
                     IRFHEEDARPMGRPAAGVHGIRLAAGDRVMAADVIDAETREFDLLTVSDSGFGKRTQL
                     TEFNRQYRGGQGVTAMKLTAKNGKVAGAYIVNTDQELMLISTAGVVIRMPVAQISRYG
                     RQTQGVSVMRLGNGEKVASLTVLSERADESDALVDTLSEMDAAENGNGNGRARSKSNG
                     RVPRASKAKRG"
     gene            complement(2253..3038)
                     /locus_tag="VFV93_11240"
     CDS             complement(2253..3038)
                     /locus_tag="VFV93_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165961.1"
                     /translation="MPDLPVAGDWRDRVPPALVRMLTGLTFIALPAFEIRLGGLALPV
                     ICVVLAVLNGVAGLLLGRSEAQRSWSLSATDAATLILLVPVVAVASGVEVADIRLGGA
                     TAHFAAAALAVLAVVGIVATVAMLTSVESPMSSGIALLPAALTVAAAIAAAERFAADN
                     LGQGLTAAWMIAALATLLDGAMPVRYRPPVPPVAFAVFAIVVAIAGSSGGADVSRSNA
                     TIAFLTTAATGAALLLVPAGTLRARLFVTEPIRAHVESLNRRS"
     gene            3234..3845
                     /gene="nadD"
                     /locus_tag="VFV93_11245"
     CDS             3234..3845
                     /gene="nadD"
                     /locus_tag="VFV93_11245"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX5165962.1"
                     /translation="MSSTRQVALFGGTFDPVHIGHLIIAEIMRHHLRLDQVVFLPAGR
                     PPHKPDQILSSDDDRLAMLRLAIADAPAFTISTIDIERPGASYTADSLRVIQDLAGPA
                     CQLHFLMGQDSLRDFPTWHRPDLIAGLARLVVALRPGVSVSVDQIEEAVPAAKDRIQL
                     VPVPLIGISSRELRGTIRAGGPYRYQVLPSVADYIEQHRLYQD"
BASE COUNT          753 a         1320 c         1198 g          628 t
ORIGIN      
        1 ggccagggca acttcggctc ggtcgacggc gacggtgcgg ccgcgatgcg ttacaccgag
       61 acgaaactct ccgccattgc cgaagagatg ttgatcgata tcgacaagga caccgtcgat
      121 ttcaagccga actacgatga tcagacccgc gagccgaaag tactgccagc caagttgccg
      181 aacttgctcg tcaacggggc gtccggcatt gccgtcggca tggcaaccaa catcccgccg
      241 cacaacctga ccgaggtctg taacggcatc atccacctga tcgaccatcc cgaagcgaca
      301 gtcgacgacc tgatgcaatt catcaccggc ccggacttcc cgaccggcgc gacgattctc
      361 ggtcgcgaag gcatcatggc cgcttacgcg accggccggg gtcgcgtcgt catccgcgcc
      421 aacgccttca tcgaggagat ggcgcgcggt aatcgctacg cgatcatcgt cagtgagttg
      481 ccctatcagg taaacaaggc cgcactgatc gaaaagatcg ccgaactggt caagatcgga
      541 cgtctcgacg gcattcacga cctgcgcgac gaatctgacc gcaatggcat gcggatcgtg
      601 atcgagctca agcgggacgc gcagccgcgc aaggtgctca acaacctctt caagcacact
      661 gctttgcaga catcgttcgg cgtcaacgcg ctcgccctcg tcgatggcga gcagccgcgc
      721 gttctgaccc tcaagcgcat cctgcaactg cacatcgagc accggcagga gattctgacc
      781 cgcaggacgc agttcgagct ggaacgcgcc aagcgccgcg cccacatcct cgaaggtctc
      841 aagatcgcgc tcgacaacct cgacgcgatc atcaagacga tccgcgcttc gcgcacgtcc
      901 gagagcgccc gcaccaacct gatgaaggac ttcaagctct ccgaggtcca ggcgacggcc
      961 attctcgaca tgcaattgcg ccggctggcc gccctggagc gcaagaagat cgaggacgag
     1021 taccgtgagg tgctcaagga gatcgcccgc ctcgaggacc tgctggcgaa tccgcgcaag
     1081 atcctcgcgt tgatcaagga cgacctgacc tatctcaagg agaagttcgg cgacgcccgc
     1141 cggacgcgaa tcgtcgagga tccgggcgag atcagcgacg acgacctgat tcccgaggtg
     1201 gatgtcatcg tcacgctgac caatcgcggt tacgtcaagc ggattccgga cgatgtctac
     1261 aagacccagc ggcgcggtgg ccgcggcgtg acgggcctga cgatgcgtga ggcggacggt
     1321 gtccagcacc tgctggcggc caactcgcac gactcgctgc tgttcttcac cgaccgggga
     1381 cgggtattcc aggtcaaggt ccacgagcta ccggatgccg gtcgaacggc caagggcgtc
     1441 ccgatcatca acctgatctc gatcctgccc aacgagtcga tcgcgacgat gctgccggtc
     1501 aaggacttcg cggagagcag ctacctgttc ttctgtaccc gcaagggccg catcaaacgc
     1561 acctcgctcg atcagttctc atccgtccga tcgtcgggcc tgattgcgat cggcctcgac
     1621 gatgacgacg aactggcctg ggtgcggacc acttccggcg acgacgagat catcctagtg
     1681 accgagcagg ccaaggcgat ccgcttccac gaggaagacg cccgcccgat gggccggccg
     1741 gccgccggtg tccacggcat ccggctggcc gctggtgatc gggtgatggc cgccgacgtg
     1801 atcgacgccg aaacacgcga gttcgacctg ctgaccgtct cggacagcgg cttcggcaag
     1861 cgcacgcagc tgacggagtt caaccgtcag taccgtggtg gccagggcgt gaccgccatg
     1921 aagctgacgg ccaagaacgg caaggtcgcc ggcgcctaca tcgtcaacac cgatcaggag
     1981 ctgatgctga tctcgacggc cggtgtcgtc atccggatgc cggtcgccca gatctcccgc
     2041 tacggccgcc agacccaggg tgtctcggtc atgcggctcg gcaacggcga gaaggtggcg
     2101 tcgctgacag tcctgtcgga gcgggccgac gaatcggacg cgctggtcga tacgctctcc
     2161 gaaatggatg ccgccgagaa tggcaacggc aacggccgag ccaggagcaa gagcaacggc
     2221 cgcgttccga gagcttcgaa ggccaagcgc ggctaacttc tccggttgag actttcgacg
     2281 tgggcccgga tcggttcggt tacgaacaac cgggcccgca gcgtccctgc cggaacgagc
     2341 agcaatgcgg caccggttgc cgccgtcgtc aggaacgcga tcgtcgcgtt ggaccggctg
     2401 acatccgcac cacctgagct gcccgcgatc gcgacgacga tcgcgaagac ggcaaacgcc
     2461 actggcggca ccggcggccg gtagcgtacc ggcatcgccc catccagcag cgtggccaac
     2521 gcagcgatca tccaggcggc cgtcagcccc tgacccagat tgtcggctgc gaaccgctct
     2581 gccgccgcga tggctgccgc gacggtcagc gccgccggta gcagcgcgat gccgctcgac
     2641 atcggcgatt caacgctcgt caacatcgcg accgtcgcga cgatgccgac aacggccagt
     2701 accgccagtg cggcggcagc gaaatgggcc gttgcgccac cgaggcggat gtcagccacc
     2761 tcgactccgg acgcgaccgc gacaaccggc acgagcagga tcagcgtcgc cgcgtcggtt
     2821 gcgctcaatg accaggagcg ttgcgcttcc gagcgcccaa gcaacaggcc ggcgaccccg
     2881 ttcaggaccg cgagcacgac gcagatgacc ggcagggcaa gcccgcccag ccggatctca
     2941 aacgccggca gcgcgatgaa cgtcaggccg gtcagcatcc gcaccagcgc cggagggacc
     3001 cggtcacgcc agtcacccgc caccggcaag tccggcatcc tcagttgtcg tcgcgtcggt
     3061 tgtcgatcgg cgttgaaggt tcgccgttcc cggacgggac gggagggtgg ccgcgtctca
     3121 tctgcgttca tcgcccgatt gtacccaaca ctgtccggcg cggcggtggc cgggaccctt
     3181 gctatactca cccgccagca gccggttgct cggatcatca ccttggaacg cgtgtgagca
     3241 gcaccagaca ggtcgccctc ttcggcggca cattcgatcc cgttcatatc ggacacctca
     3301 tcatcgccga gatcatgcgc catcacctgc gactcgatca ggtcgtcttt ctgccggcgg
     3361 gccggccccc gcacaagccg gatcagatcc tgtcctccga cgacgaccgg ctggcgatgc
     3421 tccggctcgc tattgccgac gcacccgcct tcacgatcag cacgatcgac atcgagcggc
     3481 caggagcgtc atatacggcc gactcgctgc gcgtgatcca ggatctggcc ggccccgcct
     3541 gccagttgca cttcctgatg gggcaggact cgctgcgcga ctttccaacc tggcaccggc
     3601 ctgacctgat cgccggactg gctcggctgg tcgtcgcgct ccggcccggt gtcagcgttt
     3661 cagtcgatca gatcgaggag gctgttccag ccgccaaaga tcgaatccag ctcgtgccgg
     3721 tcccattgat tggtatctcg tcacgggaac tgcgtggaac cattcgcgcc ggtgggcctt
     3781 atcgatatca ggtcctgccg tctgtcgccg attacatcga gcaacatcgg ttgtatcagg
     3841 attgagacat gtccggacgg tcagcggttg acacgtctcc gatcagccgc gtacactca
//
LOCUS       DASUGJ010000502         4495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_496712, whole genome shotgun sequence.
ACCESSION   DASUGJ010000502 DASUGJ010000000
VERSION     DASUGJ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496712"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..705)
                     /locus_tag="VFV93_11250"
     CDS             complement(184..705)
                     /locus_tag="VFV93_11250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5165963.1"
                     /translation="MSDIIIRAMEPTDIPRLTEIYGQRQVASMTLQVPFTPEAERATR
                     WGTSDSHRMLVAVVDETVAGNGGLTLYARRRAHAGSIGMGVDQACQGRGIGTALLEAL
                     IDLADNWYNLRRLELEVYTDNEPALRLYKRFGFVIEGTHRAYAWREGDWADAYTMARL
                     RDEPRLMKDDRQS"
     gene            complement(716..1180)
                     /locus_tag="VFV93_11255"
     CDS             complement(716..1180)
                     /locus_tag="VFV93_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165964.1"
                     /translation="MLLQLIRPRGGTVSDGIRLAAINGFGLIVSALLIGFISLILRWL
                     DFPVLLIGFGLVVILVAAAVVFWFRYASAHGKAARTSQRNVRPDVFGAIAAIPFALVG
                     LFLVANGLFGLFLAMVTFSLSRAGDGLERLLFAVIFLGLAATNIIIARAASD"
     gene            1391..1546
                     /locus_tag="VFV93_11260"
     CDS             1391..1546
                     /locus_tag="VFV93_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165965.1"
                     /translation="MSDSEAGGGGRNALVLVVALLVLGAVLAMLYKSASNRDLPDDEW
                     FDFEHPS"
     gene            complement(1714..3432)
                     /locus_tag="VFV93_11265"
     CDS             complement(1714..3432)
                     /locus_tag="VFV93_11265"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5165966.1"
                     /translation="MRYSRLFGKTLRDAPAGTETVSAGLMVRAGFVRQVAAGIHSYLP
                     LAVRSLAKIEAIIREEMESIDCHELLMPVVQPADLWQETGRWSKIDDALVRFADRGDR
                     QMVLAMTHEEVATDLVRREVRSYRQLPVQFFQIQTKFRDEPRPRAGLLRGREFIMKDA
                     YSFHTDAGDLQRFYDACHAAYLRAFQRCGLPVLVVEAAAGIMGGSGSHEYMLETPAGE
                     DVLLVCPNGDYAANREVATTRWPVSHEEPLPVEEVATPDAATIEAVASYLGVSTDRTA
                     KAVFYDADGQLVFVVIRGDVKVNEDKLASLLSAGTLQPASEAMIRASGAVPGYASPVG
                     LHDVTVVADLSAQAPNLVAGANREGYHLRNVNLGRDYQPTHLADIALVEAGAACPVCG
                     AALVETRGIEVGNIFKLETVYSVPMAANYLDEHDREQPIVMGSYGFGVSRTLAAIAEQ
                     HHDDRGLIWPVTVAPFNVHLVVLGADDTARTLADSVEASLDAAGIDVLYDDRDESAGV
                     KFADADLIGIPIRLTVSQRSLKAGGIEIKRRDSGPETATTVAPDAIVEHVQRLLTELG
                     RQYLPN"
     gene            complement(3575..>4495)
                     /gene="aspS"
                     /locus_tag="VFV93_11270"
     CDS             complement(3575..>4495)
                     /gene="aspS"
                     /locus_tag="VFV93_11270"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEX5165967.1"
                     /translation="GKQAASRVMAQLAASYRAALNRRGFVEIFTPKIVGSATEGGANV
                     FPVTYFDRPAFLAQSPQLYKQMMVGVHERVFEVGPVFRAEPHATTRHLNEYISLDLEF
                     GFIHDHRDVMALVEALLREMMDELRGACAAEIALFESTIPEVTTIPIVGFREAQEIIL
                     RLYGEDCRHEPDLAPQHERWLGEWAKQEHGSDFLFVEGYPTEKRPFYTMPSPEDSAVS
                     NSFDLLFRGLELITGGQREHRYDRLVEVMARRGVDPAPFEGYLDAFRFGMPPEGGCAV
                     GLERFVAQLLGAANVREVTAFPRDINRLAP"
BASE COUNT          788 a         1526 c         1437 g          744 t
ORIGIN      
        1 gagggcgact gggccgacgc ctacacgatg gcgcgcctcc gggacgagcc gcgcctgatg
       61 aaggacgatc gccaatcata ggcctcctgt cgtcctgagc cgcagtgaag gacctctctg
      121 cgctaacacc atgcccggtc aaagcagaga ggtccttcgc tgcggctcag gacgacagga
      181 ggcctatgat tggcgatcgt ccttcatcag gcgcggctcg tcgcggaggc gcgccatcgt
      241 gtaggcgtcg gcccagtcgc cctcgcgcca ggcgtaggcg cggtgggtac cttcgatgac
      301 gaagccgaag cgtttgtaga gtcgcagggc gggctcgttg tccgtgtaga cctcaagctc
      361 cagccggcgc aagttgtacc agttgtcagc caggtcgatc agtgcctcca gtagcgccgt
      421 cccaatcccc cggccctggc atgcctgatc gacgcccatt ccgatcgacc cggcgtgtgc
      481 gcggcgtcgc gcgtagagcg tcaggccacc gttgccggca accgtctcgt cgacgacggc
      541 caccagcatc cgatggctgt cgctcgtgcc ccagcgtgtc gcccgctctg cctccggcgt
      601 gaacggcacc tgcaacgtca tcgacgcgac ctgtcgctga ccgtagatct cggtgagccg
      661 tggaatgtct gtcggctcca tggcccggat gatgatgtcg ctcatcggat gcacctcaat
      721 cgctggctgc tcgggcgatg atgatgttcg tcgccgccag gccgaggaag atgacggcaa
      781 acagcagtcg ctccaagcca tcaccggccc gcgagaggct gaaggtcacc atcgccagga
      841 acaggccgaa cagcccgttc gccaccagaa acagccccac cagcgcgaac ggaatcgccg
      901 caatcgcacc gaagacatcc ggccggacat ttcgctgcga ggtgcgcgcc gccttgccgt
      961 gcgcggacgc gtaacgaaac cagaagacga ctgccgccgc gacgaggatg acgacgaggc
     1021 cgaagccgat cagcaggacc ggaaaatcga gccagcgcag aatcagcgag atgaacccga
     1081 tcagcagagc ggagacgatc agcccgaagc cgttgatcgc cgccagccgg atcccgtcac
     1141 tgaccgtgcc accgcgtggc ctgatcagtt gcagcaacat ctcccggtcc ccttgtggac
     1201 ctcccgaata ctcggaatac atctgcgtcc ctcgtgactg gttgccatgc gtccgttgcg
     1261 atgattgtac tgtccactgt cacaccgaat tcgcgaagag gttgctatac tcgcacccga
     1321 acgcctcacg cggacggcgc acccgttctt cgacaaaccg cgccgtcttc agcaggaacg
     1381 gagggatccg atgagcgatt cggaagccgg cggcgggggc cgcaacgcgc tcgtactggt
     1441 cgtcgcactg cttgtgctcg gcgcggtact ggcgatgctc tacaagtcgg ccagcaaccg
     1501 cgatcttccg gacgacgagt ggttcgactt cgagcatccg tcgtagcaac aggtaacgtt
     1561 cgatgcgcag gggccggctt tcgccggtcc ctgttcgttt ccccacgcac gcccccctgt
     1621 cgtcctgagc ggcagcgaag gacctctccg cgctaacgcc atgacctgcc agggcggaga
     1681 ggtccctcgc tgcggctcaa ggcgacagag aattcaattc ggcaggtact ggcggcctag
     1741 ctcggtcagt aaccgttgga cgtgctcgac aattgcgtca ggagcgaccg tcgtcgctgt
     1801 ctcgggtccg gaatcacgac gcttgatctc gatgccgccc gccttcagcg agcgctggct
     1861 gaccgtcaag cggatcggga taccgatcag gtccgcgtcg gcgaacttca ctccggccga
     1921 ctcgtctcga tcgtcgtaga gcacgtcgat gccggccgca tccaaagacg cctcgaccga
     1981 atcggcaagg gttcgcgctg tatcgtccgc cccaagcacg accaggtgaa cattgaacgg
     2041 tgcgaccgtc acgggccaga tcagcccccg gtcgtcgtga tgctgctcag cgatggccgc
     2101 cagtgtccgc gagacgccga agccgtacga gcccatgacg attggctgct cccgatcgtg
     2161 ttcgtcgagg tagttcgcgg ccatcgggac cgagtaaacc gtctccagct tgaagatgtt
     2221 gccgacctcg atcccgcgcg tctcgaccag tgccgcaccg cagaccgggc aggccgcgcc
     2281 ggcctcgacc agcgcgatgt cggccagatg cgtcggctgg tagtcgcggc cgaggttgac
     2341 gttgcgcagg tggtagccct cacggttcgc gccggccacc agattcggcg cctgggcgga
     2401 gaggtcggcc acgaccgtga catcgtgcag cccgaccggc gaggcataac ccggcacggc
     2461 gccgctggcg cgaatcatcg cctcgctggc cggctggagc gttccggcgc tgagtagcga
     2521 cgcgagcttg tcctcgttga ctttgacatc gccgcgaatc acgacgaaga ccagttgccc
     2581 atccgcgtcg tagaagacgg ccttggcggt ccggtcggtg ctgacgccca gatacgacgc
     2641 gacggcctca atcgtcgccg catccggcgt cgcgacctcc tcgaccggca gcggctcctc
     2701 atggctgacc ggccagcgcg tcgtcgccac ctcgcggttg gcggcgtagt cgccgttcgg
     2761 gcagaccagc aggacgtcct cgccggccgg tgtctcgagc atgtactcgt gtgagccgct
     2821 gccgcccatg attccagccg ccgcctcgac gaccagcacc ggcaggccac agcgctggaa
     2881 ggcgcgcaga taggcggcgt ggcaggcatc gtagaaacgc tgcaagtcgc ctgcgtcggt
     2941 gtggaacgag taggcatcct tcatgatgaa ctcgcgcccg cgcagcaggc cggcccgtgg
     3001 acgcggctcg tcgcggaatt tggtctggat ctggaagaac tggaccggaa gctggcggta
     3061 ggaacgcacc tcgcgccgga cgaggtcggt cgcaacctcc tcatgcgtca tcgccagcac
     3121 catctggcgg tcgccacgat cggcgaaacg caccagcgcg tcgtcgatct tcgaccagcg
     3181 ccccgtctcc tgccagagat cggccggctg gacgaccggc atcagcagtt cgtggcagtc
     3241 gatcgactcc atctcctcgc gaatgatcgc ctcgatcttg gccagcgacc gaaccgccag
     3301 cggcaggtat gaatgaatgc ccgccgccac ctgccgcacg aaccccgcgc gtaccatcag
     3361 gccggcactg accgtctcgg tcccagccgg ggcatcacga agggtctttc caaacaacct
     3421 gctgtagcgc atcgccgcct ctctttgcgt ctggtggcga actgtagcag acgttcgggt
     3481 atcgggagtc gggattcggg gcttaggaac gtcccggttc ctggtagaac gcgctcatgt
     3541 cccagccccg aatcccgatt cccgattccc gacgctacgg cgccagccga ttgatgtctc
     3601 gcgggaaggc cgtcacctcg cgaacgttgg cggcgccgag cagttgggcg acgaagcgtt
     3661 cgaggccgac ggcgcagccg ccttcggggg gcattccgaa gcggaaggcg tccaggtagc
     3721 cctcgaacgg tgccggatcg acgccacgtc gcgccatgac ctcgaccagc cggtcgtagc
     3781 gatgctcgcg ctggccgccg gtgatcagct caaggccgcg aaacagcagg tcgaacgagt
     3841 tggagacggc cgagtcttcc gggctgggca tcgtgtagaa cggccgcttc tcggtcgggt
     3901 agccctcgac aaacaggaag tcgctgccgt gctcctgctt cgcccactca cccagccagc
     3961 gctcgtgctg tggcgcgagg tccggctcgt ggcggcagtc ctcgccgtac aaccggagga
     4021 tgatctcctg cgcctcgcgg aagccgacga tcgggatcgt cgtgacctcc ggtatcgtcg
     4081 actcgaacag cgcgatctcg gcggcacacg ccccgcgcag ctcgtccatc atctcgcgca
     4141 gcagcgcttc gaccagcgcc atcacgtcgc ggtggtcgtg aatgaagccg aactccaggt
     4201 cgagcgagat gtactcgttg agatgccggg tcgtcgcgtg cggctcggcc ctgaagaccg
     4261 gcccgacctc gaagacccgc tcgtggacac cgaccatcat ctgcttgtag agctgtggcg
     4321 actgcgccag gaaggcgggc cggtcgaaat acgtcaccgg gaagacgttg gcgccgccct
     4381 cggtggctga gccgacgatc ttcggcgtga agatctcgac gaagccgcgc cggttcaacg
     4441 ccgcgcggta cgacgccgcc agttgcgcca tcacccgcga ggcggcctgc ttgcc
//
LOCUS       DASUGJ010000503         7203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_219089, whole genome shotgun sequence.
ACCESSION   DASUGJ010000503 DASUGJ010000000
VERSION     DASUGJ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219089"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..796)
                     /gene="dtd"
                     /locus_tag="VFV93_11275"
     CDS             complement(329..796)
                     /gene="dtd"
                     /locus_tag="VFV93_11275"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX5165968.1"
                     /translation="MRAVVQRVSSAAVDIGGERVAEIGPGLLILVGATHGDSDDDARQ
                     LAGKAANLRIFADDAGKMNLSILDKGYDAIVVSQFTLCGDIRKGRRPSFIDAAAPDLA
                     APLVDRFAEELRSLGLRVGTGRFGAEMAVSLVNDGPVTIWMDSAELRRQSSAG"
     gene            complement(796..1275)
                     /gene="ispF"
                     /locus_tag="VFV93_11280"
     CDS             complement(796..1275)
                     /gene="ispF"
                     /locus_tag="VFV93_11280"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX5165969.1"
                     /translation="MSRVGIGYDIHPFATGRRMVLCGIEFPGEAGLAGHSDADAALHA
                     VSDALLGAAGLGDIGEHFPPTDERWRDADSGDLLRAVVAQLSPRFTIGNVDLTIVAER
                     PKVGPRRDAMRARLAELLGIPVDRVNVKATTNERLGALGRGEGLAALAVALLNEQEQ"
     gene            complement(1272..1961)
                     /gene="ispD"
                     /locus_tag="VFV93_11285"
     CDS             complement(1272..1961)
                     /gene="ispD"
                     /locus_tag="VFV93_11285"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX5165970.1"
                     /translation="MTTPATLGVVIVAAGSGVRFGDAGKALASLAGRPLLSWSLALFD
                     SLPETHEIVVVAGSHTLDSCTALLTTASLAHVTVVEGGATRTASMLAGLAALSDQVSH
                     VAVHDAARPLVTVALAQRVIAAAVATGAAVPVIPVRDTLHVVSAEGRIAATPDRATLR
                     AAQTPQVARRDWLLAAGRSAAGSTDEGGMLHAAGYPVALVEGDPDNLKITWPQDLALA
                     EALLAARKVAR"
     gene            complement(1958..3037)
                     /locus_tag="VFV93_11290"
     CDS             complement(1958..3037)
                     /locus_tag="VFV93_11290"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX5165971.1"
                     /translation="MQDGDPQGRIGRSPFDRVLRYVGLVIGGLIGWQIGSEIGGTQED
                     VHWAAYAALIACVLGGIAFIITPYVTVGLFRWLRRKLSEIPAIDIVAAGIGLIIGGIL
                     SSILALPSALLPDPLGQIMPFVVAVCVCVLSVLVMIVQKNELLPLLTRRGGPVMPEER
                     LLLDTSVIIDGRIVELMRSRIVNFPLVVPGFILRELQGIANSDDPSRKVRGRRGLDVL
                     ERLQREGIASVEMLEVEVEEEQAVDNKLIRLAKLSRYHILTGDQNLERVAKLQDVVAI
                     NINTVAQAMRPPVAPGEELELSIVQAGREHGQGIGYLDDGTLVVIEEGRDRIGKQVRV
                     VVTRTLQTGTGRMAFAQVKEEVPVG"
     gene            complement(3233..4099)
                     /locus_tag="VFV93_11295"
     CDS             complement(3233..4099)
                     /locus_tag="VFV93_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5165972.1"
                     /translation="MSSPAASGAAAHADLHTHSTASDGTLAPADLVRQAHRRGLSILG
                     LTDHDTTAGLAEARATGQALGVRVIPGIELSTDVSAGEVHMLGYGVDPDDDTLQTALA
                     SYRQSRRERAERIIERLRELGVDLPEGSVKAPEGDASIGRPHIARAMIVAGYVETISE
                     AFDRYLGDGKPAYIASERKPTPVEAIRLIRAAGGLPIHAHPFSSVEFPATLPDLITAG
                     LAGIEVYYAEYTPERRRELARIAAEYNLLATGGSDYHGERFKERRDLGSVELPADVVR
                     RFLARLDAQPTD"
     gene            complement(4104..4382)
                     /locus_tag="VFV93_11300"
     CDS             complement(4104..4382)
                     /locus_tag="VFV93_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HEX5165973.1"
                     /translation="MQVQRNGEVLKVSARSRPSAVAGAIAGVVREAGRAEVQAIGAGA
                     TNQAVKAVAIARDYLLETGIEAVCLPAFIDVTIDDEDRTAIRLIVEPR"
     gene            complement(5078..6610)
                     /gene="rny"
                     /locus_tag="VFV93_11305"
     CDS             complement(5078..6610)
                     /gene="rny"
                     /locus_tag="VFV93_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX5165974.1"
                     /translation="MMYVLIAIVSAVVAGALAYFLTKSKLESDAASTVGRAEHEAKRV
                     VEEAEAKRRETVLEAKDEAIRLRTEMDREVSNRRRELERVERRIEQKEEGLDRKNEQV
                     ERRDKNLQRREKELDTRESELETYRQRQDEELQRVAMLTTDQARDILLSRTEQEMRDM
                     LSRRVHELEQEAKAEADRRSRKILATTIQRIASDYVAETTVSVVPLPSDDMKGRIIGR
                     EGRNIRALEQATGVDLIVDDTPEAVTLSGYDPVRREVARRSLMKLIQDGRIHPARIEE
                     VVSKTRLELEQVMREEGEKVAYEANVQGLHPDLIKLLGRMKFRTSYGQNVLQHSLEVS
                     MIAGGLAAELGADVNVAKTAALLHDIGKAVDHEVDGPHALIGADIARRLGRSARIVHA
                     IAAHHGEEEPQTVEAFIVATADAISGARPGARREMVETYIKRLEALEAVANSFEGVEK
                     SFAIQAGREVRIMVSPDKIDDLAATKLARDIVKKIEENLEYPGQIKVTVIRETRAVDY
                     AR"
     gene            6876..7148
                     /locus_tag="VFV93_11310"
     CDS             6876..7148
                     /locus_tag="VFV93_11310"
                     /inference="COORDINATES: protein motif:HMM:NF039544.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HEX5165975.1"
                     /translation="MSRRNRLIALGVVVLFVVALLDQLGRHPEDRDWHGKVLGFPYDF
                     RMPTLERLQQRVWNPDDERIIVPHVVGIGWTVNLYQLKRRLQLLIA"
BASE COUNT         1179 a         2409 c         2336 g         1279 t
ORIGIN      
        1 aatgcagcct ggtcggtatc ctgctcgtcc ggttgcccgg cctcacctgg cgcgcgattg
       61 ttgttgggct gcactctgac ctccgcaagc atgcaggcag cgtttcgggt tgtactcgtc
      121 tgccggacgg tagacggtcg cgtggtggcg gcgagtacaa ctgtcctgac gagcccgact
      181 ataacatagc gcctttggcc cggagtctag cccagaagta acgaaaggct cgtctaacca
      241 cctgcacgtt cgctcgtacg tacaccctgt tggatggccg gcgaactggc tggcagactc
      301 acgcgtcgcg atgcggcaag tgctgcctct agccagcaga tgactgccga cgcagctcgg
      361 cgctgtccat ccagatcgtc accggtccat cgttgacgag gctgacggcc atctcggcgc
      421 cgaagcggcc agtgccgacc cgcaggccga gggagcgcag ttcctcggcg aagcggtcga
      481 ccagcggcgc ggccaggtcg ggcgcggcgg cgtcgatgaa cgacggacgc cggcccttgc
      541 gaatgtcgcc acaaagcgtg aactgcgaga cgacgatcgc gtcgtagccc ttgtcgagga
      601 tcgagaggtt catcttgccg gcgtcatcgg cgaagatgcg caggttggcg gccttgccgg
      661 ccagctgcct tgcgtcgtca tcgctgtcgc catgggttgc accgaccagg atcagcaggc
      721 cggggccgat ctccgccacc cgctcgccgc cgatatcgac cgccgccgaa ctgacccgct
      781 ggacgaccgc tctcatcact gctcctgctc gttcaacaac gcgaccgcca gcgcggccag
      841 tccctcacca cgaccgagcg cgccgagccg ctcgttggtc gtcgccttga cgttgacgcg
      901 atcaaccggg atgcccagca attcagccag ccgtgcccgc atcgcgtccc ggcgcgggcc
      961 gaccttcgga cgttcagcga cgatcgtcag gtcgacgttc ccgatcgtga agcgcgggct
     1021 cagttgagcg acgaccgccc gcagcaggtc gccgctatcg gcatcgcgcc agcgttcgtc
     1081 ggtcggtggg aagtgctcgc cgatatcgcc gaggccggct gcgccgagca gcgcgtcgct
     1141 gacggcatgc agggccgcgt cggcatccga gtggccggcc aggccggcct cgcctgggaa
     1201 ctcgattccg caaaggacca ttcgccggcc cgtcgcaaac gggtggatgt cgtagccgat
     1261 gccaacccgg ctcatcgcgc gacctttcga gctgccagca gtgcctcggc cagcgccaga
     1321 tcctgcggcc aggtgatctt gaggttgtcc gggtcgcctt cgaccagcgc gaccgggtaa
     1381 ccggccgcgt gcaacatgcc gccctcgtcg gtcgagccag ccgcgctccg gccggcagcc
     1441 aacagccagt cgcgccgggc aacctgcggt gtctgcgccg ctcgcaacgt cgcgcgatcc
     1501 ggcgtcgccg cgatccggcc ctcggcgctg acgacgtgga gtgtgtcgcg gaccgggatg
     1561 actggaaccg ccgccccggt cgcgacggcg gcggcgatca cccgctgggc gagggcgacc
     1621 gtcaccagcg ggcgggcggc gtcgtggacg gcgacgtgac tgacctgatc gctgagcgca
     1681 gccagtccgg ccaacatcga ggccgttcgc gtcgcaccgc cctcaacgac cgtcacgtgt
     1741 gccagcgatg cggtcgtgag caatgctgta cagctgtcga gcgtgtggga gccggcgacg
     1801 acgacgatct cgtgtgtctc aggcagcgag tcgaacagtg ccagcgacca gctcagtagc
     1861 ggtcgcccgg ctaaggaggc gagcgccttg ccggcgtcgc cgaagcggac acccgagccg
     1921 gccgcgacga tgacgacgcc gagggttgct ggggtcgtca tccaaccggg acttcctctt
     1981 tgacctgggc gaaggccatc cggccggtac cggtctggag cgtccgtgtc acgacgacgc
     2041 gcacctgctt gccgatccgg tcgcgcccct cctcgatgac gaccagcgtg ccgtcgtcaa
     2101 ggtaaccgat gccctggccg tgctcgcggc cggcctgaac gatcgacagc tcaagctcct
     2161 cgccgggcgc gaccggcggg cgcatcgcct gggcgacggt gttgatgtta atcgcgacga
     2221 catcctggag cttggcgacc cgttccaggt tctgatcgcc ggtcaggatg tggtagcgcg
     2281 agagcttggc gagccggatg agcttgttgt cgaccgcctg ctcctcctcg acctcaacct
     2341 ccagcatctc gaccgatgcg atcccctcgc gttgcagccg ttcgagaaca tccagcccgc
     2401 gccggccgcg caccttgcgg ctggggtcgt cggagttcgc gatcccctgc aactcgcgca
     2461 ggatgaagcc cggcacgacc agcgggaagt tgacgatccg gctacgcatt agttcgacga
     2521 ttcgtccgtc gatgatgacg ctcgtgtcga gcagcaggcg ctcctccggc atgaccgggc
     2581 caccgcgtcg ggtcagcagc ggcagcagct cgttcttctg gacgatcatc accagcaccg
     2641 acaggacaca gacgcagacg gcgacgacaa acggcatgat ctgtccgagc gggtcgggga
     2701 gcagcgccga gggtagcgcg aggatcgacg agaggatgcc accgatgatc agtccaatcc
     2761 cggccgcgac gatatcgatg gcagggatct cggataactt gcggcgcaac cagcggaaca
     2821 atccgacggt aacgtacggg gtgatgatga aggcgatgcc gccgagcacg caggcaatca
     2881 gcgcagcata ggcggcccag tggacatcct cctgcgtgcc gccaatttcg ctgccgatct
     2941 gccagccgat caggccgcca atgaccagcc cgacgtagcg tagcacccga tcgaatgggg
     3001 agcgaccgat gcgtccctgc ggatcaccat cttgcacgga aaatgtccac caacgacctc
     3061 tcaagccccg gcatgccaac agggtccggg caacgtcaat cgcgggcgag gagatgggct
     3121 gatcaagccc tggaatccgc gggaagcaac cgcaaaccgg ctcgttgccc agcggccccc
     3181 gcaggcgatg atgctcggca gtctatcacc gcaacgcagg ccggggctat cgtcagtctg
     3241 ttggctgggc gtcgagacga gcaagaaatc ggcgcactac gtcggccggc aactcgaccg
     3301 aaccgagatc gcggcgctcc ttgaagcgct cgccgtggta atcgctcccg ccggtcgcca
     3361 gcaggttgta ctcggccgcg atccgcgcca gttcccggcg ccgctcgggg gtgtactcgg
     3421 cgtagtagac ctcaatcccg gccaggccgg ccgtgatcaa gtccggcagg gtggcgggaa
     3481 actcgaccga ggagaacggg tgggcgtgga tcggcagccc gccggcggcg cggatcagcc
     3541 ggatcgcctc gaccggcgtc ggtttgcgct cgctggcgat gtaggcaggc ttgccgtcgc
     3601 cgaggtagcg atcgaacgcc tcgctgatcg tctcgacata gccggcgacg atcatggccc
     3661 gcgcgatgtg cggcctcccg atcgacgcgt caccttccgg cgccttgacg ctgccctccg
     3721 gaagatcaac gccgagttcg cgcaggcgct cgatgatccg ctcggcgcgc tcgcgacggg
     3781 actgccggta gctcgccagt gccgtctgga gcgtgtcatc gtcgggatcg acgccgtagc
     3841 cgagcatgtg gacctcgccg gcgctgacgt cagttgagag ctcgattccg gggatgacgc
     3901 gcacgccgag cgcctggccg gtcgcccttg cttcggccag gccggcggtc gtgtcgtgat
     3961 cggtcaggcc gagaatggaa agcccccgcc ggtgggcctg gcgaaccagg tcggcggggg
     4021 cgagcgttcc atcagaggcg gtcgagtgcg tgtgcaggtc ggcgtgtgcc gctgcgccgc
     4081 tcgccgccgg gctgctcaag tggctagcgt ggttccacga tcagccgaat ggccgtgcgg
     4141 tcctcgtcgt cgatcgtgac gtcgatgaag gccggcaggc agacggcttc gatgccggtt
     4201 tcgagcaggt agtcgcgagc gatcgcgact gccttgacgg cctggttcgt cgcgccggcg
     4261 ccgatggcct ggacctccgc acgacccgct tcgcgaacga caccagcgat ggcgccggca
     4321 accgcgctcg gccgtgaccg ggcggagacc ttcagcacct cgccattgcg ctgtacctgc
     4381 accggcgtct cgacggcgac ggcgacctgt ccttcggcgc cattcgagta ttccggctgg
     4441 ctggcgacat ctgtatcgat gctatcggcg taggcgtcct cgtccggtgg aaacttggcc
     4501 tcggggtcga tcttgatgtc gcgcaggtcc agcttgctaa agaaccgatc catgagaatc
     4561 ctccatcagg gcggcgaggc cgctctggct gttgaagcag taccgtagtg tgtgctttca
     4621 agcttgaatg catgtgcgag tgcgatcgtc tgctctacgc atacctccgt tactcagcaa
     4681 tcaagcgcga caccgatgct tgaccgtgtc acgacggctc gcacccaata accctctcca
     4741 tgctccgatg ttccccgccg cgcatgccat catccccaac cagcgcgctc aacgggttcc
     4801 acttgtggaa tccgggagcc actcgcgggt gcaacgacat cgctgcgcaa tgcacggagc
     4861 gggaccttcc ccccattcga taccacccct gcaacgacgt gccagccagg ctcgacgagc
     4921 gcgtcgtacc cctgcagatt ggtatcgaag gtagtcatac caccccttcg cggccaccgt
     4981 caaccatatg cggaagcgtg caagcagcca gaaaagacac aacgccgtgg cgctgatgca
     5041 ccacggcgtt gtcacagttc acagggattc gaccggatca gcgggcgtag tcgaccgcgc
     5101 gtgtctcacg gatgacggtc accttgatct ggcctgggta ttcgaggttc tcctcgatct
     5161 tcttgacgat atcacgggcc agcttggtgg ccgccagatc gtcgatcttg tcgggcgaga
     5221 ccatgatacg gacctcacgg cctgcctgga tcgcgaacga tttctcgacg ccttcgaatg
     5281 agttcgcgac cgcttcgagc gcctccaggc gcttgatgta ggtctcgacc atctcgcgtc
     5341 gtgcgccggg gcgcgcgccg ctgatggcgt ccgccgtcgc cacgatgaaa gcctcgaccg
     5401 tctgcggctc ttcctcaccg tggtgggcgg cgatggcatg gacgatccgc gccgagcggc
     5461 ccagccgccg ggcgatgtcc gcgccgatca gtgcgtgggg gccgtcaacc tcgtgatcca
     5521 ccgccttgcc gatgtcgtgc aggagcgcgg cagtcttggc gacgttgaca tccgccccga
     5581 gctcagcggc caggccaccg gcgatcatcg agacctccag cgagtgctgg agaacattct
     5641 gaccgtagga tgtccggaac ttcatccggc cgaggagctt gatcaggtca gggtgcaacc
     5701 cctggacatt ggcttcgtag gcgaccttct cgccctcttc acgcatcacc tgttccagct
     5761 cgaggcgcgt cttggagacg acctcctcga tccgggccgg gtggatccgg ccgtcctgga
     5821 tcagcttcat cagcgaccgg cgggccacct cgcggcggac cgggtcgtag ccggagagcg
     5881 tgacggcttc cggtgtgtcg tcgacgatca ggtcgacacc ggtggcctgc tcaagcgcgc
     5941 ggatgttgcg accctcgcgc ccgatgatgc gtcccttcat gtcgtcgctc ggcagcggga
     6001 cgaccgagac ggtcgtttca gcgacgtagt cgctggcgat ccgctggatc gtcgtcgcca
     6061 ggatcttgcg ggagcggcga tcggcctcgg ctttggcctc ctgctccagc tcatggacac
     6121 gccggctgag catgtcccgc atctcctgct cggtacggct gaggaggatg tcgcgcgcct
     6181 ggtcggtcgt cagcatggcg acgcgctgca gctcctcgtc ctggcgctgg cggtaggtct
     6241 ccagctccga ttcgcgggtg tcgagctctt tctcccgacg ctggaggttc ttatcgcggc
     6301 gttccacctg ctcgttcttg cggtcgagcc cttcttcttt ctgctcgatc ctgcgctcca
     6361 cccgctccag ctcgcgccgg cggttagaga cttcgcggtc catctccgtt cggaggcgga
     6421 tcgcctcgtc cttggcctca aggacggttt cgcgccgctt cgcctccgct tcctcgacaa
     6481 cgcgcttcgc ctcatgctct gcccggccga ccgtcgatgc tgcatcagat tccagcttcg
     6541 acttggtcag gaagtaggca agggcgcccg ctactaccgc ggaaacgatg gcaatgagaa
     6601 cgtacatcat tctcgttgct ccttattccg atatggggct tcagttgtta atgtactgga
     6661 gatcgttccg ggtaccccgt ggacgcggtc cggagaatcc gaacgtatgc gccccgtgag
     6721 cgaaacatct caagcgtcaa cctgttgatg ataggtgtgc tttgatacac atgtcaagcg
     6781 aagcaagcgt acgtacaacg tgatcgcact ctttttgtga ccggtaacgt cgaagatggt
     6841 agcggaacgg atgggcgcga aaggggagtg tggagatgtc tcgcagaaac cggctgatcg
     6901 cgttgggcgt cgtcgtcctg ttcgttgtgg cgctcctcga tcagcttggc cgtcatcccg
     6961 aggaccgcga ctggcacggc aaggtgctcg gcttcccata cgacttccga atgccgacgc
     7021 tggagcgcct gcaacaacgc gtctggaacc ccgacgacga gcgcatcatc gtcccgcacg
     7081 tcgtcggcat cggctggacc gtcaacctct accagctcaa gcgccggctg caactgctga
     7141 ttgcgtagtc tcccgttgtc gtcctgaggc cgcagccgaa ggacctctcc gcgttagccg
     7201 ccc
//
LOCUS       DASUGJ010000504         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1884770, whole genome shotgun sequence.
ACCESSION   DASUGJ010000504 DASUGJ010000000
VERSION     DASUGJ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884770"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /locus_tag="VFV93_11315"
     CDS             complement(<1..1670)
                     /locus_tag="VFV93_11315"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX5165976.1"
                     /translation="MAHPFLERIRTGPLLCDGAMGTMLHNNGVSLDACFDALNLTNRD
                     DILTIHRDYIAAGADIIETNTFGANRFRLAEHRLEDRVRDINFRGAKLASEAREVSGV
                     PVLIAASIGPTGRAQMPYGLIDPEGVRAAFREQVEALLEGGVDLLMFETISALDEMVE
                     AIAAAREVADLPIVASMTFTDDGRTLAGHTPQEVINTLQGLGVDAIGVNCSVGPRRVL
                     DVLAEMREYAAGVALSGLPNAGWPMQVGDRVLYPSSPDYFAHYATEALDLGVRVVGGC
                     CGTTPAHIQAMRAAIDERTRAGRPVAISAPPTRKIELLPPEEPTQLARKIGRDFVVSV
                     ELDPPRGLNPEKMLAGARLLKDSGVDAVNVADSPMARVRMSAQALCFLVQTEVGVETI
                     LHFTTRDRNLMGLQSDLLGAHALGVRNILALTGDPPSLGDYPNATAVYDIDSTGLAKV
                     LNSMNAGTDALGSPIGMQASFTIAVAVDPTRDDLPQEADRLHRKIGGGAHLVMTQPVY
                     EMATWRDFLAIYEDRHGPLMVPVMLGILPLQSHRHTEFLYNEVPGIRPT"
     gene            1843..2409
                     /locus_tag="VFV93_11320"
     CDS             1843..2409
                     /locus_tag="VFV93_11320"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5165977.1"
                     /translation="MASEEDVWVSRAKAGDQAAFEAIVQRYERQIYNFVYRMMGDPDD
                     AYDLTQDCFVRAYRSLGNTNTELNVSAWLHRIASNACLDVLRRRKRIRWLPWDNTKHE
                     HLLEDGGVDDPERNIVARETSTVVQDTLNKMSPRNRAALVLREYEGLSCEEIGDILGL
                     SRSAVKSVLFRGREEFRKLYVAVDGEAS"
BASE COUNT          453 a          838 c          787 g          418 t
ORIGIN      
        1 gtcggccgaa tccccggaac ctcgttgtag aggaactcgg tgtggcggtg actctgcaag
       61 gggaggatgc ccagcatgac tggaaccatc agcgggccat gccggtcctc gtagattgcc
      121 aggaagtcgc gccaggtcgc catttcgtag accggctggg tcatcaccag gtgcgccccg
      181 ccgccgatct tgcgatgcag ccggtccgcc tcctgcggca aatcgtcccg cgtcgggtca
      241 accgcgacgg cgatcgtgaa gctggcctgc atgccgatcg gcgagccgag cgcgtctgtg
      301 ccggcattca tcgagttcag caccttcgcc agcccggtcg agtcgatgtc gtagaccgcc
      361 gtcgcgttcg ggtaatcacc gaggcttggc gggtcgccgg tcaacgccag gatgttgcgc
      421 acgcccagtg catgtgcgcc gagcaggtcg gactggagac ccatcagatt ccggtcgcgg
      481 gtcgtgaagt ggaggatcgt ctcgacgccg acctcggtct ggaccagaaa gcagagcgcc
      541 tgcgcgctca tccgcacccg cgccatcggc gaatcggcga cgttgacggc atcgacgccg
      601 gagtccttca gcagccgcgc gccggccagc atcttctcgg ggttcagtcc acggggaggg
      661 tcaagctcga ccgacacgac gaagtcgcgg ccgatcttgc gcgccagctg cgtcggctcc
      721 tccggcggca acagctcgat cttccgcgtt ggcggcgcgc tgatcgcaac cggccttccc
      781 gcgcgggtac gctcgtcgat cgccgcccgc atcgcctgaa tgtgcgccgg cgttgtcccg
      841 cagcagccgc cgaccacccg cacacccagg tcgagcgcct cggtcgcgta gtgggcgaag
      901 taatcgggcg aagagggata gagcacccgg tcgccgacct gcatcggcca gccggcgttg
      961 ggcaatcccg agagggcgac gccggccgcg tactcgcgca tctcggccag cacgtccagc
     1021 acccgccggg gaccaacgct acagttgacg ccgatcgcat caaccccaag cccctggagt
     1081 gtattgatga cctcctgcgg cgtgtggccc gccagcgtcc ggccgtcgtc agtaaacgtc
     1141 atcgacgcta cgatcggcag atccgccacc tcgcgggccg ccgcgatcgc ctcgaccatc
     1201 tcatccagcg cgctgatcgt ctcaaacatc agcaggtcga cgccgccttc gagcagcgcc
     1261 tcgacctgct cgcggaaggc cgcccggacg ccctctgggt caatcaaccc atacggcatc
     1321 tgcgcgcggc cagtcggccc gatcgaggcg gcgatcagga ccggcacacc tgaaacctcg
     1381 cgcgcctcgc tggccagttt ggcgcctcgg aaattgatgt cgcgcacccg gtcttccagt
     1441 cgatgctccg ccagccggaa gcggttcgcg ccgaacgtgt tcgtctcgat gatgtcggcg
     1501 ccagccgcga tgtagtcgcg atggatcgtc agaatgtcat cccggttggt caggttcaac
     1561 gcgtcgaaac aggcatccag cgacacgccg ttgttgtgca gcatcgtccc catcgcacca
     1621 tcgcagagga gtggtcccgt tctgatacgt tcgagaaatg gatgcgccat tggggtatcc
     1681 ttccggggaa ccagttgatg gacagactcg agcattccgg attgtagcac ccggtctttc
     1741 gctggaacgc gcggcccgat cgaccaacag atggcccgcg cacaaccaac ggcccactcg
     1801 tacgttgaaa ccgtacggag ggcagtggtg gaaagggtct tcatggcatc cgaagaggat
     1861 gtgtgggtaa gccgggcaaa ggccggtgat caggccgcct tcgaagcaat cgtccagcgt
     1921 tacgagcgtc agatctacaa cttcgtctat cgcatgatgg gcgatcccga tgacgcctac
     1981 gatctgacgc aggactgctt cgtgcgcgcc tatcgcagtc tgggcaatac gaacacggaa
     2041 ctcaacgtct cagcctggct gcaccgcatc gcctcgaacg catgcctcga tgtcctgcgc
     2101 cgccgcaagc gaatccgctg gctgccgtgg gataacacca agcacgaaca cctgctcgaa
     2161 gatggcggag tcgacgatcc cgaacgcaac atcgtcgctc gcgagacaag tacggtggtc
     2221 caggacacgc tgaacaagat gagcccgcgc aatcgcgccg cgctcgtcct gcgcgagtac
     2281 gagggcctct cctgcgagga gatcggcgac atcctcggcc tgtcacgttc ggccgtcaag
     2341 tcagtcctct tccgtggccg tgaagagttc cgcaagctgt atgtcgcggt agacggggag
     2401 gcctcctgat ggccgaccac aactgccgcg aggcacgcga gatcatctcg gcgttcatcg
     2461 acggcgaagc gagccgcgag gagagcgcac gtctcc
//
LOCUS       DASUGJ010000505         6244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_774595, whole genome shotgun sequence.
ACCESSION   DASUGJ010000505 DASUGJ010000000
VERSION     DASUGJ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774595"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..660
                     /gene="recR"
                     /locus_tag="VFV93_11325"
     CDS             52..660
                     /gene="recR"
                     /locus_tag="VFV93_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX5165978.1"
                     /translation="MRQSRITAEPVARLIGELGRLPGVGPKSASRLAYYLLRASREQA
                     LSLAEAILEVKERIVLCSRCYNITESDPCAICQDPGRDQRIICVVEEPLDVLALDRTG
                     EFKGLYHVLHGAISPVDGIGPDKLRIRELLERVDRERPEEVILAMNPNIEGDATAMYI
                     ARQLVASGVSISRPASGLPVGGDLEYADEITLGRALAGRRTL"
     gene            719..1783
                     /locus_tag="VFV93_11330"
     CDS             719..1783
                     /locus_tag="VFV93_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165979.1"
                     /translation="MPTLAIRPLNPLDIPAMRHVCQRRDRLDMPDAVDPAEPDLANQI
                     LATLPANVRQGRVVVALVDGELCAYMVFRPQASRFRWDLLAVAAGSPRLDATDDVCFE
                     LWSALLEYAIRQAGEQGAKRLFATVREDCAAYDSLRATGFERYARFMILAGTRQSGPV
                     ALPEGMREQEDSDVWSIHQLYHHVTPRAVQFAEALTSSVWELPQRSLFDRLTGMPQRT
                     KRFVLDTTNGVQAYCAIQVRRGGAMARLMISHEYGAQAASFVLAAAAEAGVAPASRLR
                     ILVPGYTGELVSGFVEAGFEMEDERTAMVRHTTAPAVVHAKLAPIPADAGERVPRGIP
                     TYVRNARRAIPEQGARNPCA"
     gene            1801..3552
                     /locus_tag="VFV93_11335"
     CDS             1801..3552
                     /locus_tag="VFV93_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEX5165980.1"
                     /translation="MTTIQPQHVADPDDMPLNAEIAITDNLDDLLAVLPERIADAVRR
                     IMSERDDPGLIELVLDLGRRPEARFQVGAVRLTEDEVTREDLSQVALRIGRFGDDNRA
                     GIERTLHRISAIRNRTGDIVGLTCRVGRAVYGTIGIVSDLVESGESILLLGRPGVGKT
                     TMLREVARVLADDLEKRVVVVDTSNEIAGDGDIPHPSIGRARRMQVPTPALQHAVMIE
                     AVENHMPEVIVIDEIGTELEAAAARTIAERGVQLIGTAHGNTLENLMMNPTLSDLIGG
                     IQSVTLSDEEARRRGTQKSILERKAPPTFGVMVEIRERDVVAVVRDVEGAVDAILRGQ
                     QQRSELRERGEDGEVTITDIAPARPERVERPERAERHPARTPRRDEPRIIESGGFEPE
                     DDESGQVQRNPLARGRGTPQRPVRIFPFGVSRNRLEQAIGALALPAVIVRDVHEADMV
                     LTLKNYYRQRPQQLREAEARGVAIYVLRANTVVQMVNVLRDLVREVRPDDLPDAEPLQ
                     PPPSGDGDPITNAMYEAEEAITAVLDGAPPIELRPQAPYIRRLQHQLADRYNLGSRSR
                     GREPHRHVEIFREGGWG"
     gene            3549..4199
                     /gene="tmk"
                     /locus_tag="VFV93_11340"
     CDS             3549..4199
                     /gene="tmk"
                     /locus_tag="VFV93_11340"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX5165981.1"
                     /translation="MSLFITFEGTEGSGKTTQIGMLAQSLRGSGYQVVTTREPGGTEL
                     GEALRRLLLESETAIGPEAEAYLMTGARAEHVRHVIRPALERGDVVLCDRFVDSTFAY
                     QGGGRRLPVHELRLMQSLAIGETTPDLTILVDVPAEVGLERRAKAREVNRIDRESLEF
                     HARVAFWYRQEARANPDRWVVVDGSRSPADVHVAILRNVIERLNTDGVVRHRSGER"
     gene            4196..4531
                     /locus_tag="VFV93_11345"
     CDS             4196..4531
                     /locus_tag="VFV93_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX5165982.1"
                     /translation="MKLIFAVVQGKDADNLRSALLDAGYRTTQINSAGGFLRESNATF
                     LVGVDNEAVQDVLRIVKQTCYTRTQYVNPLLPIMEPGEFYMPNPVEVQVGGAAVFICN
                     VNRYERIFS"
     gene            4570..5319
                     /locus_tag="VFV93_11350"
     CDS             4570..5319
                     /locus_tag="VFV93_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5165983.1"
                     /translation="MDEALIARIFQLFLALAGAYLLALWFALVVWTYRDITSRSTNAF
                     TQIFSTLVVVLFFVPGAIIYLILRPRETLDEAFQRSMEEEYLLQDLDDIPLCPICRRA
                     IRDDFVFCPHCSAELRHACNTCHRLVDVRWEVCPYCGSDQSLDREPEPRELIRETAGS
                     RSRQRAATAPLQAIDGGRAGQRPDSGNRRPRQLREDEALAEPAGDSLVEAHDDAESTD
                     EHPATTPNGVPVRNRGGRARATGRRRGESGN"
     gene            complement(5258..6112)
                     /locus_tag="VFV93_11355"
     CDS             complement(5258..6112)
                     /locus_tag="VFV93_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX5165984.1"
                     /translation="MGTPVTTIAVVAASDKRSSHQLAPSIGRWLEQRGCTVVGEDDLR
                     AGARADAVVALGGDGLMMRMAHSFPSMPLLGINFGKVGFLAMVERAQWQEALTRVLAG
                     DYVIQDGPTLASSVKRGKQQIDGGWAINDIVVRSGMQMIDVELYVDGRYVNTYPGDGM
                     IVASPQGSTAYCMAAGGPILTAGVRGFAITPISAHSPIRATLVVPEESRIELAVTSDR
                     DAYLILDGHLEQELQRLDVVTVSRGREQFRLIITEHMNFYTAFQSKFNYLIRPGAVPS
                     LGHDRPDS"
BASE COUNT         1081 a         2020 c         2063 g         1080 t
ORIGIN      
        1 ccaccgcaat gtatactttt tgccggatgc agcggctggt ggaggctcgg attgcggcaa
       61 tcgagaatta cagcagagcc agttgccagg ttgatcggcg agctcggccg gttacctggt
      121 gtcggtccca aatccgcgtc ccgtctcgcg tattacctgc tgcgtgcgtc ccgtgaacag
      181 gcgctatcac tggccgaggc gatcctcgag gtcaaggagc ggatcgtcct ctgctcacgc
      241 tgttacaaca tcactgagtc ggacccgtgc gcgatctgcc aggacccggg tcgggatcag
      301 cgcatcatct gtgtcgttga ggagccactg gacgttctcg cgctcgatcg gaccggtgag
      361 ttcaagggtc tctaccacgt cctgcacggc gcgatttcgc cggttgacgg gatcgggccg
      421 gacaagctgc gcatccgcga gttgctggaa cgggtcgatc gggagcggcc agaagaggtc
      481 atcctggcga tgaacccgaa tattgagggc gatgcgaccg ccatgtatat cgcccgccaa
      541 ctggttgcct cgggcgtgtc gatcagcagg ccggccagcg gcctgccggt tggtggcgac
      601 ctggaatacg cagacgagat tacgctcggc cgcgccctgg ccggccggcg cacgttgtag
      661 cgggccgggt tcgacatttg ctgtcggatc gccccgtgaa cgagagattc ggacacctgt
      721 gccaactctc gccatccgtc cactgaatcc actcgatatc ccggcgatgc ggcacgtctg
      781 ccagcgtcgt gatcggctcg acatgcccga cgcggtcgat cctgccgagc cggatctggc
      841 gaaccagatc ctggcaacgc tgccggccaa tgtgcgacag ggccgcgtcg tcgttgcgct
      901 cgtcgatggt gagttgtgtg cctacatggt cttccggcct caggcatcgc ggtttcgctg
      961 ggacctgctc gcggtcgcgg ccggctcgcc gcgcctggat gcgaccgacg acgtctgttt
     1021 cgagctgtgg agcgcattgc tggagtacgc gatccggcag gccggcgagc agggggcgaa
     1081 gcggttgttc gcaaccgttc gcgaggattg cgctgcgtac gacagcctgc gcgcgaccgg
     1141 ctttgagcgc tacgctcgct tcatgatcct ggccgggacg cgccagagcg ggccggtcgc
     1201 attacccgaa gggatgcgcg agcaggaaga cagcgatgtc tggtcgatcc accagctcta
     1261 ccatcacgtg acgccgcgag cggttcagtt cgccgaggcg ctgaccagca gcgtctggga
     1321 actaccgcag cgcagcctgt tcgacaggct gactggcatg ccccagcgga cgaagcggtt
     1381 tgtgctggac acgaccaatg gcgtccaggc ctactgtgca atccaggtcc gacggggtgg
     1441 cgcgatggcg cgactaatga tctctcacga gtatggcgcc caggcagcct cgttcgtgct
     1501 ggcagctgcc gctgaggccg gcgttgcccc ggcaagccgg ttgcgtatcc tcgtaccggg
     1561 ctacaccggc gaactggtct ccggctttgt cgaagccggc tttgagatgg aggacgaacg
     1621 gaccgccatg gtgcgtcaca caaccgctcc ggccgtcgtc cacgccaagc tggcgccgat
     1681 tccggccgat gcgggcgaac gggtaccgcg cggcatacca acctatgtgc gcaatgcacg
     1741 gagagcgatc cctgagcagg gagcccgcaa tccatgtgcg tgaatggagc gaatgacgca
     1801 ttgacgacga ttcagccaca acacgtggcc gatccagacg atatgcccct gaacgccgag
     1861 atcgccatca ccgacaacct cgacgatctg ctggcagttc tgcctgagcg aatcgccgat
     1921 gctgtccgac ggatcatgtc cgagcgggac gatcctgggc tgattgagct ggttctcgat
     1981 ctgggccgcc ggccagaggc acgttttcag gtcggagccg ttcggctgac cgaggatgag
     2041 gtgacccgcg aggatctgtc gcaagttgcg ctgcggatcg ggcgcttcgg cgacgacaac
     2101 cgggccggca tcgaacggac cctgcatcgc atctccgcga tccgcaaccg gaccggtgac
     2161 atcgtcggtc tgacatgccg ggttggccgg gccgtctatg ggacgatcgg catcgtcagc
     2221 gacctcgtcg agtccggcga gagcatcctg ctgctcggcc gtccgggcgt cggcaagacg
     2281 acgatgctgc gcgaggtggc gcgtgtcctc gccgacgacc ttgagaagcg ggtcgtcgtc
     2341 gttgacacca gcaacgagat cgcgggcgat ggtgacatcc cgcatccgtc gatcggtcgc
     2401 gcccgtcgga tgcaggtgcc gacgccggcg ttgcagcatg ccgtcatgat cgaggctgtc
     2461 gaaaaccaca tgccggaagt gatcgtcatc gacgagatcg gcaccgaact ggaggccgcc
     2521 gccgcccgga cgatcgccga gcgcggcgtc cagctgatcg gcaccgccca cggcaacacg
     2581 ctcgagaacc tgatgatgaa cccgacgctg tccgacctga tcggcggtat ccagtcggtg
     2641 acactctccg acgaagaagc gcgccggcgc gggacgcaga agtcgattct ggaacggaaa
     2701 gcgccgccga cgttcggtgt catggtcgag attcgcgagc gggatgtcgt cgccgtcgtg
     2761 cgtgatgtcg agggcgccgt cgacgcgatc ctgcgcggcc agcagcaacg ctctgagctg
     2821 cgcgagcggg gcgaggacgg cgaagtcacg atcaccgata tcgcgccggc ccggcccgaa
     2881 cgcgtcgagc gcccggagcg agcggagcgg cacccggcca ggacgcctcg acgcgacgag
     2941 ccgcgcatca tcgagagtgg cggattcgag ccggaagatg acgaaagcgg ccaggttcag
     3001 cgcaatccgc tggcgcgcgg ccgtgggacg ccccagcggc ccgtcaggat ctttccgttc
     3061 ggcgtcagcc gcaaccggct ggagcaggcg atcggcgcgc tggcgttgcc ggccgtcatc
     3121 gtgcgcgatg tccacgaggc cgacatggtg ctgacgctca agaactacta ccggcagcga
     3181 ccgcagcagc tccgcgaggc cgaggcacgc ggtgtcgcca tctacgtcct gcgcgccaac
     3241 acggttgtcc agatggtcaa tgttctgcgc gacctcgtcc gtgaggtacg accggacgat
     3301 ttgccagacg ccgaaccgct ccagccgcca cccagcggtg atggtgatcc gatcacgaat
     3361 gcgatgtacg aggcggagga agcgatcacg gccgtcctcg atggcgcgcc gccgatcgag
     3421 cttcggccgc aggcgccgta tatccggcgc ttgcagcacc agcttgccga tcgctacaac
     3481 ctcggatcgc gcagccgtgg ccgtgagccg caccggcacg tcgagatctt ccgtgagggc
     3541 ggctgggggt gagcctgttc atcacctttg agggcaccga gggatccggc aaaacgaccc
     3601 agatcgggat gctggcccaa tcgctgcgcg gcagcggcta ccaggtcgtg acgacccgcg
     3661 agccgggcgg aacagagctt ggcgaggcac tgcgacggct gctgctcgaa tccgagacgg
     3721 cgatcggccc ggaggccgag gcgtacttga tgaccggcgc acgggccgaa cacgtcaggc
     3781 acgtcatccg tccggcgctg gagcggggcg atgtcgtcct ctgcgaccgc ttcgtcgact
     3841 cgaccttcgc ctatcagggc gggggccggc gcctgccggt ccacgagctg cggctgatgc
     3901 agtcgctggc gatcggcgag acgacgccgg atctgacgat cctggtcgat gttccggcgg
     3961 aggtcggcct ggagcggcgg gcgaaggcgc gcgaggtcaa ccgaatcgac cgcgagagcc
     4021 ttgagtttca cgctcgtgtc gcattctggt atcgtcagga ggcacgcgcc aatcccgacc
     4081 gctgggtcgt tgttgacggc tctcgatctc cggcggacgt gcatgtcgcg atactgagga
     4141 atgtgatcga gcgtctcaac acggacggcg tcgtccggca caggagtgga gagcgatgaa
     4201 gctgatcttc gccgtcgtcc agggcaagga cgccgacaac ctgcgttcgg cgttgctcga
     4261 cgccggctat cggacgacgc agatcaacag cgccggtggc tttctacgcg agagcaacgc
     4321 gaccttcctg gtcggagtcg acaacgaagc cgtccaggac gtcctgcgca tcgtcaagca
     4381 gacctgctac acgcgcacac agtacgtcaa cccgcttctg ccaatcatgg agccgggcga
     4441 gttctatatg cccaatccgg tcgaggtgca ggtgggcggg gccgcggtct ttatctgcaa
     4501 tgtcaatcgc tacgagcgca tattctcctg atagccatgc atgcgcggcc cgcccgaagg
     4561 gaaccctgat tggacgaagc tctcatcgcg cggattttcc agctttttct ggcactggcg
     4621 ggcgcctacc tgctggccct ctggttcgcg ctcgtcgtct ggacctatcg cgacatcacg
     4681 tcgcgctcga cgaatgcctt cacgcagatc ttctcgacac tggtcgtcgt tctcttcttc
     4741 gttcccgggg cgatcatcta cctgatcctg cgcccccgcg agacgctcga tgaagccttc
     4801 cagcgctcga tggaagagga gtatcttctc caggacctcg atgacatccc gctctgcccg
     4861 atctgccgca gggcgatacg ggatgacttc gtcttctgcc cgcattgcag cgccgagttg
     4921 cggcatgcct gcaatacctg tcatcgtctc gtcgatgtgc gctgggaggt ctgtccgtat
     4981 tgcggctccg accagtcgct cgatcgtgag ccggaaccac gcgagctgat tcgcgagacc
     5041 gctggatcgc gaagccgcca gcgggcggcg accgcgccac ttcaagcgat tgacggcgga
     5101 cgggccggcc agcgacccga ttccggcaat cgccgcccgc gacagcttcg cgaggatgag
     5161 gccctcgccg agccggctgg cgattcgctc gtcgaggcac atgacgacgc ggaatcgacc
     5221 gacgagcatc cggccacgac tcccaatggc gtcccggtca ggaatcgggg cggtcgtgcc
     5281 cgagcgacgg gacggcgccg gggcgaatca ggtaattgaa cttgctctgg aaggccgtgt
     5341 agaagttcat gtgctcggtg atgatcagcc ggaactgttc gcggccacgg ctgaccgtca
     5401 cgacatccag ccgctgtagt tcctgctcca gatggccatc gaggatcaga taggcgtcgc
     5461 gatcgctggt gacggccagt tcgatccggc tctcctccgg cacgaccagc gtggcccgaa
     5521 tcggcgagtg cgccgaaatc ggcgtaatcg cgaagccgcg tacccccgcc gtcagaatcg
     5581 ggccaccggc ggccatgcaa taggcagtac tgccctgcgg cgatgcgacg atcatgccgt
     5641 cacccggata ggtattgaca tagcgcccgt cgacatacag ctcgacgtcg atcatctgca
     5701 tcccgctgcg gacgacgatg tcgttgatcg cccaaccgcc gtcgatctgc tgcttgcctc
     5761 gcttgacgga cgacgccagc gtcgggccgt cctggatcac gtagtcacct gccagcaccc
     5821 tggtcaacgc ctcctgccac tgtgcgcgct cgaccatcgc caggaagccg accttgccga
     5881 agttgatgcc gagcagcggc atgctgggaa aggaatgcgc catccgcatc atcaggccgt
     5941 cgccgccgag agccacgact gcgtcggctc gcgcgccagc gcgcaggtca tcctcaccaa
     6001 cgaccgtgca gccgcgttgc tccagccagc gcccgatcga cggcgccaac tggtgcgacg
     6061 agcgtttgtc actggccgcg acgacggcga tcgtcgtaac aggcgttccc atcagatgat
     6121 ccgtccgacg acttcgatcc cgttctgggc cattgagtgc aggaagacat ggtgcagcag
     6181 gtctccgtca tgcggtggcg cgccgagcgt tcgcgccgtc tcgaccggga tatcgtaggt
     6241 ctcg
//
LOCUS       DASUGJ010000506         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_634519, whole genome shotgun sequence.
ACCESSION   DASUGJ010000506 DASUGJ010000000
VERSION     DASUGJ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634519"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..470
                     /locus_tag="VFV93_11360"
     CDS             72..470
                     /locus_tag="VFV93_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165985.1"
                     /translation="MTTMATLDWAMQALGISIVPAGRSEAPACTIGLDGRSIDAEVIV
                     IAAAPDIQRPIVVASIQPTDVRLVMLERDAPDNVCPPVEHAQGRSDVIHVAHGLTATG
                     DWFLLADLCEAVACREPPAGDIITANARDN"
     gene            518..1252
                     /locus_tag="VFV93_11365"
     CDS             518..1252
                     /locus_tag="VFV93_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5165986.1"
                     /translation="MAHVVVVEDESEIQALIVGALREEGHTVETFADGRDALERLTGP
                     GMSQPDLIVLDLMLPNVDGLAITREVRRTRITPILMLTAKSTELDRVLGLELGADDYL
                     TKPFSIRELQARVGAILRRVEMMRLQSRVESDTELPIDHGDLRIDPTRREVLVDGSPV
                     ALTAREFDLLHLLASNPGRAFSRDYLLERLWRDDFEGFDRTVDTHILRLRRKLGGSGS
                     AAERIVTLWGVGYKYDLPAGADRTTP"
     gene            1249..1563
                     /locus_tag="VFV93_11370"
     CDS             1249..1563
                     /locus_tag="VFV93_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165987.1"
                     /translation="MSALKWILTLPLRAIRYPFRLYRRKISVQLIVSHILVVFLSIVL
                     LEAALIGIAIAIFQNLEDDAFTEYSIATTRHHPRSCWRRIRRSERSRPTPGRLRWTSN
                     ND"
     gene            1542..2906
                     /locus_tag="VFV93_11375"
     CDS             1542..2906
                     /locus_tag="VFV93_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5165988.1"
                     /translation="MDEQQRLNTALAALAGQERTDTFEVTVGINAQPPPSQLGLIDGA
                     LITNTDGTIVATSDESWAASGTPVERVDFPLVSDVSRQVIARNGAPDINDNLYSIDAV
                     DRVTVASYPLVVDGEFVGVLTLQSDRIEIPAFAGFVAGIAVANGVILAAIGLPALLVS
                     IPFGIWRAHRLSKRLGDLSDTATAMGDGDLSRRVVIRGEDEVARVSLRFNDMVDRLQT
                     TDRARRSFVANVSHELRTPVAIIQGNLERLIADANQNDRPDDERRTLDTLHRETLTLS
                     RLIDDLFTLARIEETTLPIEATALQLSIVAGDVVDGIRDVAWQQRKVSVESLVAADLP
                     LVLADRTRLRQILGNLLYNALRHTPEGGLIVVNAEPVGGMLQVTVSDTGTGMNPDDLA
                     RVFDRFYQVERSGRHAEGSGLGLAIVKQLVEAQGGTISAESVAGAGTTFRFTLPLAPA
                     PVPH"
     gene            3068..>3345
                     /locus_tag="VFV93_11380"
     CDS             3068..>3345
                     /locus_tag="VFV93_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5165989.1"
                     /translation="MIDSELDDMEIEPLVELALTEKRVEIVLDACSIRGTLTSSQARV
                     SDHLNAGDAPLCIHDARVVTREGEPMADRSTVYVNKSVILFVVDLTPRP"
BASE COUNT          594 a         1085 c         1068 g          598 t
ORIGIN      
        1 gcgatacttg ccgcatatgc ttggtgacag tggttcgagg ttaccgcgtc acgaggggca
       61 tggtgcaggc gatgacgacg atggcgacgt tggactgggc gatgcaggcg ctggggatca
      121 gtatcgtccc ggcggggcgc agcgaggcac cggcctgcac gatcggactg gatggccgca
      181 gtatcgatgc ggaggtcatc gtcatcgcgg ccgcgccgga tattcaacgc ccaatcgtgg
      241 tggccagcat tcagccgaca gacgtccgtc tcgtgatgct ggagcgtgat gcgccggaca
      301 acgtctgccc tccggtcgag cacgcgcagg gacgctcgga tgtcattcac gtcgcccatg
      361 ggctgacagc aacgggcgac tggttcctgc tggccgatct gtgtgaggca gtcgcctgcc
      421 gcgagccacc ggccggggat atcatcacgg ccaacgctcg cgacaattga gcgttgcgag
      481 tatttccgga gcgaggaagc gggatcacgg cagggttatg gcacacgtcg tcgtcgtaga
      541 ggatgaatcg gaaatccagg cgctgatcgt cggcgccctg cgcgaagagg gacacacggt
      601 cgagacattt gccgatggcc gagacgcgct cgagcgcttg accggtcccg ggatgagcca
      661 gccggacctg atcgtcctcg acctgatgct gcccaacgtc gatggtcttg cgatcacccg
      721 cgaggtacgc cgcacacgca tcacgccgat cctgatgctg accgccaagt cgaccgagct
      781 ggaccgcgtc cttgggctgg agcttggcgc tgacgactac ctgaccaagc cgttctcgat
      841 ccgcgagctg caggcacggg tcggcgccat tttgcgccgg gtcgagatga tgcgactgca
      901 atcacgcgtc gaatcggaca ccgagctacc gatcgaccat ggggatctgc ggatcgaccc
      961 aacccggcgt gaggtactgg tcgacggcag cccggtcgcc ttgacagccc gcgagtttga
     1021 cctgctccac ctgctggcgt cgaacccagg ccgggcgttt tcccgcgatt acctgctgga
     1081 acggctctgg cgcgatgatt tcgagggctt tgatcgaacg gttgataccc acatcctgcg
     1141 cctgcgtcgc aagctcggtg ggtcgggatc ggccgccgaa cggatcgtca ccctctgggg
     1201 tgtcggctac aagtacgacc tgccggccgg ggctgaccgg acaacaccgt gagcgcgctc
     1261 aagtggatcc tgacgcttcc cttgcgcgcg atccgatacc cgttcagact ctaccgtcgc
     1321 aagatcagcg ttcagctgat cgtctcccac atcctggtcg tcttcctctc gatcgttctc
     1381 ctcgaggcgg cactgatcgg gatcgctatc gccatcttcc agaacctgga agacgacgcc
     1441 ttcaccgagt actcgatcgc cactacgcgt catcatccgc gatcgtgctg gcggcggatc
     1501 cggcgatcgg aacgctcgcg accgacgccc ggccgcttac gatggacgag caacaacgac
     1561 tgaacacggc gctggcggcg ctcgccggcc aggaacgaac tgatacgttc gaggtgaccg
     1621 tcggcatcaa cgcacaaccg cctccgtcgc aactcggact gatagacggc gcgctcatca
     1681 ccaacaccga tgggacgatt gtggcgacct ccgacgagag ctgggctgcg tcgggaactc
     1741 ccgtcgagcg cgttgacttt ccgctcgtca gcgacgtgtc ccgccaggtc atcgcgcgca
     1801 acggggcgcc ggatatcaac gacaatctgt acagcatcga tgcggtcgat cgggtaacgg
     1861 tggcttccta cccgctagtc gttgatggcg agttcgttgg ggttctcacg cttcagagcg
     1921 accggatcga gataccggcc tttgccgggt tcgtcgccgg aatcgccgtc gccaacggcg
     1981 tcattctggc ggcgatcggc ctgccggcgc tgctggtctc gatccctttc ggcatctgga
     2041 gggcacaccg attgtcgaag cggctcggcg atctgtccga cacggcgaca gcgatgggcg
     2101 acggagatct ctcccggcga gtcgttatcc gcggtgagga tgaggtggcg cgcgtcagcc
     2161 tgcgcttcaa cgacatggtc gaccggctcc agacgaccga tcgggcccgt cgctcgtttg
     2221 tcgccaacgt ctcccacgaa ttacggacgc cggtcgccat tatccagggc aacctcgaac
     2281 ggctgatcgc cgacgcaaat cagaacgaca ggccggacga cgaacgccgc acgctcgaca
     2341 cacttcaccg cgagacgctg acactctcgc gcctgatcga cgacctgttc acactggcgc
     2401 gaattgagga gacgacgctc ccgatcgagg cgacggcact gcagctcagc attgtggccg
     2461 gcgatgtcgt cgacggaatc cgcgacgtcg cctggcagca gcgcaaggtc tcggtcgagt
     2521 cgctggtcgc cgccgatctg ccgctggtac tggcggaccg gacccggctg cggcagattc
     2581 tcggcaacct gctttataac gcgttgcggc acacgccgga gggcggactg atcgtcgtca
     2641 acgccgagcc ggttggcggg atgttgcagg tcactgtcag cgataccggc accggcatga
     2701 atcccgacga cctggctcgc gtcttcgacc gcttctatca ggtcgaacgc tccggacggc
     2761 acgctgaagg ctccggcctc ggcctcgcga tcgtcaagca actcgtcgag gcccagggcg
     2821 ggacaatcag cgctgaaagc gtcgccggcg ccggcacgac cttccgcttc acgctcccgc
     2881 tcgcgccggc acccgtccct cactgacctc actgccttct cgcgctgaag tgatggccgc
     2941 agccgtaccc ctcgtcgcct acgacgcgat cggtgtgatc aggaccgttg gccctgcctg
     3001 agggacgctg gttgtctact acaatggcgg taggccacgg attgtcctgg aatgagggtg
     3061 cagaacgatg atcgattccg aactggacga catggagatc gagccgctcg tcgaactcgc
     3121 gttgaccgag aagcgggtcg agatcgtgct ggatgcctgc tcgatccgtg ggacgctaac
     3181 gtcatcacag gcgcgggtca gcgatcacct caacgccggc gacgcgccac tctgcatcca
     3241 cgatgcgcgc gtcgtgacgc gcgagggcga accgatggcg gacagatcca ccgtgtatgt
     3301 caacaagtcg gtcatcctgt tcgtcgtcga tctcacgccg cgacc
//
LOCUS       DASUGJ010000507         3950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2025545, whole genome shotgun sequence.
ACCESSION   DASUGJ010000507 DASUGJ010000000
VERSION     DASUGJ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025545"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFV93_11385"
     CDS             <1..465
                     /locus_tag="VFV93_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX5165990.1"
                     /translation="GLNESEAARERLAGPAPFDSARSLASALLEPHRSYFPVVRNLLA
                     GAHVAGMAHITGGGLPGNVSRIIPEGATARIDTSRWRIPELFSFVAEAGNVSVAECFR
                     AFNMGIGYVVICPPDDVPNVISIAQDAVVIGEVVAAGDERRVRLTQDGAELK"
     gene            526..1083
                     /locus_tag="VFV93_11390"
     CDS             526..1083
                     /locus_tag="VFV93_11390"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX5165991.1"
                     /translation="MFDRLDPTLTSLIVSTEESEAITACLKRLMDETGATHIMLLDRA
                     GQIISWQGGESRMQEMMHLGALLAGAYATSREMAKLLKEDGFRVLVQEGMREKIFTET
                     VEDRWLLVVVFDHQAHLGLVKVLAKRATQSLTGILHLVIEQNESRRAQFRGGIRKAAV
                     DTIDLLFNDDSAPSPGQTTEKKPWP"
     gene            1074..1649
                     /locus_tag="VFV93_11395"
     CDS             1074..1649
                     /locus_tag="VFV93_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HEX5165992.1"
                     /translation="MALINVAAREIHGKIVYYGPGLCGKTTNLQFIHGKIPQNGRSDL
                     LSIATETERTLFFDFLPLDLGKVHGFTIRFHLYTVPGQILYERTRVAVLNGADGVVFV
                     ADGQRDRLADNLQSLRELAHNLTNQGKRLLEFPLVMQYNKMDLPTALPTPVLDRYLNT
                     IKVPRFEAVAVKGTGVFETLRAISKLVVNKL"
     gene            1715..2329
                     /locus_tag="VFV93_11400"
     CDS             1715..2329
                     /locus_tag="VFV93_11400"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5165993.1"
                     /translation="MEPVLTDDEPIALLDRLGVEVPDREHAIRCAQFAGAIFDGAQER
                     LELEPSDRRLAVVAALFHDSGYLRGARDHHRKSYDILLGAGIPGFSRQEQTIAACVAR
                     YHGRTMPNIEHAGFGEMDTEEQRRTRRLSAIVRLAAACDASHLGLVSEVTARMSGDEL
                     LVTAMATGEPSVDRDRMREAAGGFQALAYLPVRIEITVQTNQKG"
     gene            2332..3864
                     /gene="purH"
                     /locus_tag="VFV93_11405"
     CDS             2332..3864
                     /gene="purH"
                     /locus_tag="VFV93_11405"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX5165994.1"
                     /translation="MPTALISVWDKTGVERLATRLAAAGFELVSTGGTARALRDAGVP
                     VIEVSDVTGFPEIMDGRVKTLHPAIHGGLLARRDKPEHLATLEQHGIRTIDVLVSNLY
                     PFADVVRRPETSDENAIENIDIGGPAMTRAAAKNHAGVVVLTDPDDYDAVLELIERDG
                     VGAVPDATRRRLAAKAFAHVATYDSLIASYLRDGGEFPAHLPVGANLLHTLRYGENPH
                     QRAAVYTVPAAGDVAGIATWHVHDGREMSYNNYLDATAAWGCALDYEAPVVVIVKHTL
                     PCGIGTSDDLVEAYERALSGDPVSAFGGIMACNRTVTEAVVEAIGRHRFDVMIAPGYT
                     SGALERLLKKRNLRVITVGNAAPVTGWELRSVPGGLLVQEPDNVAVDPPAWICATTRQ
                     PTQVELETLAFAWRGVKWVKSNAIVLAQPGVVVGVGAGQPNRVESVRIAVKVAGERAT
                     GSVLASDAFFPFPDGVEAAAAAGVTAIVQPGGSVKDAETIAVAEAHGMTMLMTGERHF
                     RH"
BASE COUNT          726 a         1202 c         1320 g          702 t
ORIGIN      
        1 gggcttaatg agagtgaggc ggcacgggag cgactggccg ggccagcgcc gttcgattca
       61 gcccgtagcc tggcgagtgc cttgctggag ccgcatcgtt cgtattttcc ggtcgttcgg
      121 aatctgcttg ccggcgcaca cgtggccggg atggcgcata tcacgggtgg cggcctgccg
      181 ggtaacgtct cgcgcatcat ccccgaaggg gcaacggcgc ggattgatac gagtcgatgg
      241 cggataccgg aactgttctc gtttgttgcc gaggcgggca atgtgtcagt agccgagtgc
      301 tttcgagcat tcaacatggg aatcggatac gtcgtgatct gtccgccgga tgatgtgccg
      361 aatgtcatca gtatcgcgca ggacgcggtc gtgataggcg aggttgtcgc ggccggcgac
      421 gagcggcgcg tcagactgac ccaggacggg gcagaactca agtgagccac gggtacagcg
      481 agcaggcgat ggtgctcgca ctggccgcgg ccggagaatt ggtccatgtt tgaccggctc
      541 gacccaacgt taacgagcct gatcgtcagc accgaagagt cggaggcgat caccgcctgc
      601 ctcaagcggc tgatggacga gacgggcgcg acccacatca tgttgctcga ccgggcgggc
      661 cagatcatct cctggcaggg tggtgagagc cggatgcagg agatgatgca tctgggagcg
      721 ctgctggccg gcgcctacgc gacatcccgt gagatggcga agctgctcaa agaagatgga
      781 tttcgtgtgc tcgtgcagga agggatgcgc gagaagatct tcaccgaaac ggtcgaggat
      841 cgctggttgc tggtggtcgt cttcgatcac caggctcacc tgggactggt gaaggtgttg
      901 gccaagcgcg ccacccagtc gctgactggt atcctgcatc tggtcatcga gcagaacgag
      961 agccggcgag cccagttccg gggcggaatc cgtaaagccg ccgttgacac gattgacctg
     1021 ctgttcaacg acgactcggc cccaagcccg ggtcagacaa cggaaaagaa gccatggccc
     1081 tgattaatgt tgccgcgcga gagatccacg gcaagatcgt ctattacggt ccaggattgt
     1141 gtggcaagac gaccaacctg cagttcattc acgggaagat cccgcagaat ggacgcagcg
     1201 atctgctctc gatcgcgacc gagacagagc gaacgttgtt cttcgacttc ctgccgctcg
     1261 atctcggcaa ggtgcatggc ttcacgatcc gctttcacct ctacaccgtg cccggccaga
     1321 ttctgtacga gcgcactcgc gtcgccgtcc tgaacggtgc cgacggtgtc gtctttgtcg
     1381 ccgatggcca gcgggaccgg ctggcggaca acctgcagag cctgcgcgaa ctggcgcaca
     1441 atctgaccaa tcagggcaag cgcctgcttg agttcccgct ggtcatgcaa tacaacaaga
     1501 tggacctgcc gacagcgctg ccgacgccgg ttctcgaccg ctatctcaac acgatcaagg
     1561 ttccgcgctt cgaggcggtc gctgtcaagg gcaccggcgt cttcgagacg ttgcgagcga
     1621 tttcgaagct ggtcgtcaac aagctgtaag ccccgcgaac ttcgatacac tctgtccgta
     1681 gccgggagtc cacccgggtg gacggagtgt tgcgttggaa cccgtactga cagacgacga
     1741 accaatcgcg ttgctggacc ggctgggggt cgaggtcccc gaccgcgagc atgcgatccg
     1801 ctgcgcccaa ttcgccggcg cgatcttcga tggcgcccag gagcgattgg aactggagcc
     1861 atcggatcgg cggctggccg ttgtcgcagc gctctttcac gactccggct acctgcgtgg
     1921 cgcgcgtgat caccaccgca agtcgtatga catcctgctc ggagctggta ttcccggctt
     1981 tagtcgtcag gagcagacga tcgccgcctg tgtcgcgcgc tatcatggcc ggacgatgcc
     2041 aaacatcgaa cacgccggtt ttggcgagat ggacaccgag gagcaacgac gcacccggcg
     2101 actgagcgcg atcgtgcggc tggcggctgc ctgcgacgcc agccacctcg gcctcgtttc
     2161 cgaggtgacc gcgcgcatgt ccggcgacga gttgctggtg acggcaatgg cgactgggga
     2221 gccgagcgtc gaccgggatc gaatgcggga agccgccggg ggctttcagg cgctggcgta
     2281 cctaccggtc cgaatcgaga tcactgtgca aacgaatcag aagggttgag gatgccgaca
     2341 gcgttgatca gcgtctggga caagaccggc gtcgagcggc tggcaacacg tctggctgct
     2401 gccgggttcg aactcgtttc gaccggcggg acggcgcgcg ccctgcgcga tgctggcgtg
     2461 ccggtcatcg aggtcagtga cgtgaccggc tttccggaga ttatggacgg tcgcgtcaag
     2521 acacttcacc cggccattca cggcggcctg cttgcgcggc gcgacaagcc tgagcacctg
     2581 gcgacgctag aacagcacgg catccgcacc atcgacgttc tggtctcgaa tctctacccg
     2641 tttgccgatg tcgttcgccg gccggaaacc agcgacgaga acgcgattga gaacatcgac
     2701 atcggcggcc cggcgatgac ccgggcagcg gccaagaatc acgcaggagt cgtcgttctg
     2761 accgatccgg acgactacga tgcggtcctg gaactgatcg agcgagacgg cgtcggcgcg
     2821 gttccagatg cgacgcggag gcggctggct gccaaggcct ttgcccacgt ggcgacctac
     2881 gacagcctga ttgcctcata tctgagagat ggtggcgagt tcccggccca cctgccggtt
     2941 ggggcgaacc tgcttcacac gcttcgctac ggcgagaacc cgcaccagcg cgcggctgtc
     3001 tataccgttc cggccgcggg agatgtcgcc ggcatcgcga cctggcacgt ccatgatggc
     3061 cgcgagatgt cctacaacaa ctacctcgac gcgacggccg cctggggctg cgcgctggac
     3121 tacgaggcgc cggttgtcgt catcgtcaag cacacgctgc cgtgcgggat cgggaccagc
     3181 gacgacctgg tcgaggccta cgagcgggcg ctcagcggcg atccagtttc cgccttcggc
     3241 ggcatcatgg cctgcaaccg gaccgttacg gaggccgtcg tcgaggccat cggccggcac
     3301 cgctttgatg tcatgatcgc gccaggctat accagcggcg cgctggaacg gctgctgaag
     3361 aagcgcaacc tgcgtgtcat cacggttgga aacgctgcgc cggtaactgg ttgggaactg
     3421 cgttcggttc ccggcgggct gctggtgcag gaaccagaca acgttgcagt tgatccgcca
     3481 gcatggatct gcgcgaccac tcgccaacca actcaggtcg agctggagac gctggcgttc
     3541 gcctggcgcg gagtcaaatg ggtcaagtcg aacgcgatcg tgctggccca gccgggcgtc
     3601 gtcgtcggcg tcggcgcggg acagcctaac cgcgtcgaga gcgtccgtat cgccgtcaag
     3661 gtggccggtg agcgggcaac cggaagcgtc ctggccagcg acgcgttctt ccccttcccc
     3721 gacggggtcg aggctgcggc cgctgccggt gtcaccgcta tcgtccagcc gggcggctca
     3781 gtcaaggacg ccgagacgat cgccgtcgca gaagcgcatg gaatgacgat gctgatgact
     3841 ggcgaacggc attttcggca ctaggcgtca cgcgtcacct acgagtccgg agccactgct
     3901 tcgaggatca tgctgaggcg gtcgtagtcg cgtttcatga gacgggcgag
//
LOCUS       DASUGJ010000508         7227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1747818, whole genome shotgun sequence.
ACCESSION   DASUGJ010000508 DASUGJ010000000
VERSION     DASUGJ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747818"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFV93_11410"
     CDS             <1..429
                     /locus_tag="VFV93_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5165995.1"
                     /translation="WASSLVTFAEDRVPLDRAELDAWLWRIDLVVGMVAERASSLSPA
                     ELDWSPPDGSWSLRQTLNHLAAAELYYVRWLDEPLPDNPVARYTTASAMVAAALPTAL
                     DAATDPNIAFAHANNADPGHCAPEEIAEQILEQEAALLAT"
     gene            753..1121
                     /gene="crcB"
                     /locus_tag="VFV93_11415"
     CDS             753..1121
                     /gene="crcB"
                     /locus_tag="VFV93_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX5165996.1"
                     /translation="MAYLWVALGGALGAMGRFAVSGVFLGRFGSGVLGTVFANISGAF
                     LIGFIMALTEERVVVSPDIRRFLTVGILGGYTTFSTWMYETHLLAGRGDVERAVLNVA
                     GSLLLGLVAVWLGVAAGRAV"
     gene            1188..1751
                     /locus_tag="VFV93_11420"
     CDS             1188..1751
                     /locus_tag="VFV93_11420"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX5165997.1"
                     /translation="MPTARTDSRFVAFLRAINVGGRTVKMDQLRALFEEIGLDNVETF
                     IASGNVIFTSSLPVADLTKRIDDHLHASLGYQAEAFVRTTAELAEIAAHQPFPESEFS
                     ENGATLHIVFLSGVPDKKTQQAVMGLRSPKDDFDIRDREVYWLIRGKMTDSTLKGPEL
                     GKALGMATTVRNVNTIRRLVAKYPPEQ"
     gene            1774..2310
                     /locus_tag="VFV93_11425"
     CDS             1774..2310
                     /locus_tag="VFV93_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX5165998.1"
                     /translation="MGREGEIVEAFIEIPQGSRNKYEYDEERGVFVLDRVLYSSVHYP
                     TDYGYIPETLAPDGDHLDILVITYNPTFPGCHVPARPIGGLDMADEKGGDFKVLAVPS
                     VDPRFDGIRSLNALEAHWLREIEAFFATYKLLEPKQTEVLGWHGHEEAWQVIDAAREA
                     YRADATGRGTRDVGRARS"
     gene            2435..4132
                     /locus_tag="VFV93_11430"
     CDS             2435..4132
                     /locus_tag="VFV93_11430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX5165999.1"
                     /translation="MHTTHDELDALLRRFGHETFRPGQARVIRDLLSGRDVMAVLPTG
                     AGKSLVFQLTAQILPGATVVVSPLIALMKDQVESLEERGFNVRLINSTQGEAESRREL
                     EYVLSKQAKILYVTPERFTNNEFMRSLRRADISLLVVDEAHCLSEWGHDFRPSYLGLG
                     SVITDLGRPTVLALTATATPWVRREIIERLGMRKPDIVVRGVDRPNLFFEVVRVESEA
                     DERRALRHMFVEGNGRDYPDELACQLKRTMQGSGIIYTATTEAARQTAQWLTEWGIPS
                     DYYHGQRRKADRERVQEGFMRGDLRVIAATNAFGLGVDKADVRFVIHRDIPGSVEAYY
                     QESGRAGRDGEFSRCTIIYRPGDLGRAAFLSGTGQISEADLQCVRFGLLKSPRTTMRQ
                     LAVDSGIANGHLARVVQLLTDLEIVTNRRGQIRMIVPDFNPSEIPLEKEERRRNYERS
                     RLEMMRVYAELNDCRREYILNYFGQEYDTEHCDMCDNDAFVAARTGKAGEPVADTLIE
                     SHPAPFALGDVVVHDAWGEGEVQRVADDSVTVLFQESGYKTLALEIVQERQLLQKLS"
     gene            4238..4606
                     /locus_tag="VFV93_11435"
     CDS             4238..4606
                     /locus_tag="VFV93_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5166000.1"
                     /translation="MAAKLDDGSPEAAYRTAAGAVFHRLRSERNWSFRDFGERVGVAH
                     TSLYAVERRETTPTIDTLAAVAAACELTLPAVLTLIIDEMTAAGDSTAAAVLQATAAL
                     NDDQRAEVLRFVEWVRFRDG"
     gene            complement(4684..5064)
                     /locus_tag="VFV93_11440"
     CDS             complement(4684..5064)
                     /locus_tag="VFV93_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5166001.1"
                     /translation="MDQKLHTTTTPGAFCPRFHYAVELIGRRWTGAILRALLSGATRY
                     SDITSAVPGLSDRLLSERLRELEAEGIVSRTVIPEMPVRIEYHLTDKGHSLESVLSSI
                     SAWAETWIEAPVAEARCDEVVRAS"
     gene            5211..5765
                     /locus_tag="VFV93_11445"
     CDS             5211..5765
                     /locus_tag="VFV93_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5166002.1"
                     /translation="MTAPATTGVTTWAIDGVHSIAEFSVKHMMVATAKGRFGSFDGTI
                     QWDQANPAASSVVVNIDVASIDTNEEKRNAHLRSDDFFNTEQYPTATFRSTRVEPDGD
                     DEFKVYGDLTIRDQTHPVVLDVELEGQVLDPYGLQRAGFEAKTEISRKQFGLNWNAML
                     ETGGAVVADKVKLLFHIEATQQQG"
     gene            complement(5834..6430)
                     /locus_tag="VFV93_11450"
     CDS             complement(5834..6430)
                     /locus_tag="VFV93_11450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX5166003.1"
                     /translation="MDEPTTPKLMIVVASNRPGRAGGPIGEWFRAKAEAHGTFAIDYA
                     DLEEINLPFMDEPNHPRLRQYTHQHTKDWSARVDAADAFVFVTPEYNHGMTAPLKNAI
                     DYLNHEWQYKPLGFVSYGGVAGGTRAVQMIKPIANVLKLTPLPEAVVVPLFQRFMTDD
                     GAFEPEELIQDAAITMLDQLVRWSNVLKPMRAGVAQPA"
     gene            6666..7184
                     /locus_tag="VFV93_11455"
     CDS             6666..7184
                     /locus_tag="VFV93_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5166004.1"
                     /translation="MTDTTTTTQPSVPAGTHIGHVHLKVADLERAVTFYRDVMGFDVV
                     ARYGTQAAFLSAGGYHHHIGLNTWESAGGDPPPSGTTGLYHFAINYPTRRDLAVAFRR
                     LLENGWQIDGASDHGTHEALYLHDPDFNGIELAWDRDPAEWPRNADGSLNMFTHRLDL
                     RALLAEAEPVTA"
BASE COUNT         1286 a         2378 c         2284 g         1279 t
ORIGIN      
        1 tgggcatcgt cgctcgtgac gttcgctgag gatcgcgtcc cgctggatcg tgcggagctc
       61 gacgcctggc tctggcggat cgacctcgtg gtcgggatgg ttgccgagcg cgccagctcg
      121 ttgtcgccgg ccgagctcga ctggtctcca cccgacggct cctggtcgct gcggcagacg
      181 ctcaaccacc tcgcggctgc cgagctgtac tacgtgcgct ggctcgacga gccgctgccc
      241 gacaacccgg tcgcgcgcta taccactgcc agcgcgatgg tcgcagcggc cctcccgact
      301 gcgctcgacg cagccaccga tccgaacatc gccttcgccc acgccaacaa cgctgacccc
      361 ggccactgcg caccagagga gatcgccgag cagatcctcg aacaggaagc tgcgttgctg
      421 gccacttgag acgttgcgtg aatcagcaca atcctcaatc ccccactccg gtacgggcga
      481 tatcgcaata tcgccctgct cccgacccga cgcgttcggg cggaacagga agggcgatat
      541 cgcgatatcg cccgtaccgg agtggaagat tgggattgag gcgactgcat cctgaatcca
      601 catgagcgta tagaatgcgc acagacggcg gtggggtccg ccggggcggg tgatccgctc
      661 gaaccgctgt gatgccgatg actcctggcc gggcgtgagc ctggccagga tttttcatgt
      721 ttcgtcggag atggggtagg aggggacgcg acatggcata tctctgggtg gcgttgggtg
      781 gggcgctcgg cgcgatgggc cgcttcgccg tctccggcgt ctttcttggc cgcttcggct
      841 ccggggtgct ggggacggtc ttcgctaaca tcagcggcgc ttttctgatc ggcttcatca
      901 tggcgctgac cgaggagcgc gtcgtcgtct caccggatat ccgccgcttt ctgacggtcg
      961 gcatcctcgg cggctacacg accttctcga cctggatgta cgagacgcac ctgctggccg
     1021 gccggggcga tgtcgagcgg gccgttctca acgtcgccgg ctcgctgctg ctcggcctgg
     1081 tggccgtctg gctgggtgtc gccgccggcc gcgccgtcta gcccggctgc ggtacatttg
     1141 acggccggat cgggttcgcg ccgcgcgcca cagccaggag tcaccagatg ccgacagcca
     1201 ggacggacag ccgctttgtc gcctttctcc gggcgatcaa cgtcggcggc cggacggtca
     1261 agatggacca gcttcgcgcg ctgttcgagg agatcgggct ggataacgtc gaaacgttca
     1321 tcgccagcgg gaatgtgatc ttcacgtcgt cgttaccagt tgcagacctc accaagcgga
     1381 tcgacgacca tctgcacgcc tcgctcgggt atcaagcgga ggcgttcgtc cggacgacag
     1441 ctgaactggc ggagatcgcg gcccatcaac cgttcccgga gtcagagttc agcgagaacg
     1501 gcgcgacact gcacatcgtc tttctatcgg gagttccaga taagaagacg cagcaggcgg
     1561 tcatgggcct gcgttcgccg aaggacgact tcgacattcg tgaccgtgag gtctactggc
     1621 tgattcgtgg aaagatgact gactcaacgc tgaagggtcc cgaattgggt aaggcgctcg
     1681 ggatggcgac gacggtccgt aatgtcaaca cgatccgcag gctggtcgcc aagtacccgc
     1741 cggaacagta gccatcgtga aaggggaccg aggatgggtc gcgaaggcga gatcgtcgag
     1801 gcgttcatcg agattccgca gggcagccgc aacaagtacg agtatgacga ggaacggggc
     1861 gtcttcgtcc tcgaccgtgt gctgtactcg tcggtccact acccgaccga ctacggctac
     1921 atccccgaaa cgctcgcacc ggatggcgac catctcgaca tcctcgtcat cacctacaat
     1981 ccgaccttcc ccggctgcca tgtcccggcc cgcccgatcg gcggcctcga catggccgac
     2041 gagaagggcg gcgacttcaa ggtgctggcc gtcccgtccg tcgatccacg cttcgacggc
     2101 atccgctccc tcaacgccct cgaagcccac tggctgcggg agatcgaggc attcttcgcc
     2161 acctacaagc tgctggagcc gaagcagacc gaagttctcg gctggcacgg ccatgaagaa
     2221 gcctggcagg tcatcgacgc cgcccgtgag gcgtaccggg cggatgctac gggacgcggg
     2281 acgcgggacg tgggacgcgc gcgttcctga ttccacgtcc cgcatcccgc gtcccgcgat
     2341 accgtgtggc ccgaatcatg cgttcgtacg atgcccggcc ctctggatgg ctgggtatgg
     2401 gccaccggcg cgcaggcgtt ggacgggatc tgcaatgcac acgacccacg acgagctgga
     2461 cgcattgctg cgtcggttcg ggcatgagac gttccggcct ggtcaggcgc gggtcatccg
     2521 cgacctgctc tccggccgcg acgtgatggc cgtcctgccg accggcgccg gcaagtcgct
     2581 cgtcttccag ctgaccgccc agatcctgcc gggcgcgacg gtggttgtct caccgctgat
     2641 cgcgttgatg aaggatcaag tcgagtcgct ggaggagcgc ggcttcaacg tccggctgat
     2701 caacagcacc cagggcgagg ccgagtcacg ccgcgaactg gagtatgtac tctcgaagca
     2761 ggccaagatt ctgtacgtga cgccggagcg cttcaccaac aacgagttca tgcgttcgct
     2821 gcgccgagcc gacatctcgc tgctggttgt cgatgaggcg cattgcctct ccgagtgggg
     2881 acatgatttc cgcccctcct acctcggtct cggtagcgtc atcaccgacc tcggccgtcc
     2941 gaccgttctg gcgttgacgg cgacggcaac gccgtgggtg cggcgcgaga tcatcgagcg
     3001 gttggggatg cgcaagcccg acatcgtcgt ccgtggcgtc gaccgaccga atctcttctt
     3061 cgaggtcgtc agagttgaat ccgaggccga cgagcgacgc gccctgcgac acatgttcgt
     3121 cgagggcaac ggacgcgact acccagacga gcttgcctgc caactgaagc ggacgatgca
     3181 gggcagcggc atcatctaca cagcgacgac cgaggccgcc cgccagacgg cccagtggct
     3241 gaccgaatgg ggcatcccgt ccgactacta ccacggccag cgccggaagg ccgaccgcga
     3301 gcgggtccag gaaggcttca tgcgcggcga cctgcgcgtt atcgccgcga ccaatgcctt
     3361 cggcctcggt gtcgacaagg ccgacgttcg cttcgtcatc caccgcgaca ttcccggcag
     3421 cgtcgaggcc tattaccagg agtccggccg ggccgggcgc gacggcgagt tctcccgctg
     3481 cacgatcatc tatcgccccg gcgaccttgg ccgagctgcc ttcctgagcg gcaccggcca
     3541 gatctccgaa gcagacctgc agtgcgtccg attcggcctg ctcaagtcgc cgcgcacgac
     3601 gatgcgccag ctcgccgtgg acagcggcat cgccaacggc cacctggcgc gtgttgtcca
     3661 attgctgacc gatcttgaga tcgtcaccaa ccggcgcggc cagatccgga tgatcgtccc
     3721 ggacttcaac ccatctgaga ttccgctgga gaaggaagag cgccggcgca actacgagcg
     3781 cagccggctg gagatgatgc gcgtctacgc ggaactgaac gactgtcgcc gcgaatacat
     3841 cctcaactac ttcggtcagg agtacgacac cgagcactgt gacatgtgcg acaacgatgc
     3901 gttcgtggcc gcgcggaccg gcaaagccgg cgagccggtg gccgacacgc tgatcgagag
     3961 ccatccagca ccgttcgcgc tcggcgatgt cgtcgtccac gacgcctggg gcgaaggcga
     4021 ggtccagcgc gtcgctgacg atagcgtcac cgtcctcttc caggaatccg gctacaaaac
     4081 cctcgccctc gaaatcgtcc aggaacgcca actgctccag aagctcagct gatctcatac
     4141 ccctacgcgg gcctacgcgg ggcgttcggc ggcatactac agcggagggc gtgatgcctt
     4201 cccctgtctg cgtgtctgaa cctgcgaagg atgattgatg gcggccaagc tggacgatgg
     4261 atctccggaa gcggcctatc ggacggcggc gggcgccgtc ttccatcgcc tgcggagcga
     4321 gcgcaactgg tcgttccgcg acttcggtga gcgggtcggc gtcgcgcaca cgtcgctcta
     4381 cgctgtcgag cgccgcgaga caacgccgac catcgacacg ctcgctgccg tcgccgccgc
     4441 ctgcgagttg accctgccgg ccgtcctgac cctcatcatc gatgagatga ctgctgctgg
     4501 ggactcaaca gctgccgccg ttctccaggc tactgccgcc ttgaacgatg accagcgtgc
     4561 tgaggtactg cgcttcgttg aatgggtgcg ctttcgggat ggataacctc ccaatccaac
     4621 aatgaatccc caatccggta cgggcgatct tgcgatatcg cccgtacggg attgggcatt
     4681 gattcagctc gcgcgtacga cctcgtcgca gcgtgcctcg gcgaccggcg cctcgatcca
     4741 ggtttcggcc caggccgaga ttgacgacag caccgattcc agcgaatgac ctttgtcggt
     4801 caggtgatac tcgatccgga ccggcatctc cgggatgacc gtccggctga cgatcccctc
     4861 ggcctccagt tcgcgcagcc gctcggagag taaccggtcg gacaggcccg gcaccgcgct
     4921 ggtgatatcg gagtagcgcg tcgcaccgct cagcagcgcc cgcaggatcg cacccgtcca
     4981 gcgacggccg atcagttcga cggcgtagtg aaagcggggg cagaacgcgc caggtgtcgt
     5041 tgtcgtgtgc agcttctggt ccatcgggac acctccatac tcccctgatc ttaccgtata
     5101 gtcgcgtact tgacaacagt gagtaaccat gctatagtaa cttacataag gttagtcaca
     5161 caccggagga gttgttccgg acggtggagg aaacaaggag tagcacgagc atgactgcac
     5221 cagccacaac cggcgtcacg acctgggcca ttgacggcgt tcactcgatc gccgagttca
     5281 gcgtcaagca catgatggtt gcgaccgcca agggccgctt cggttcgttc gacggcacga
     5341 tccagtggga tcaggccaac ccggccgcgt cgtccgttgt cgtcaacatc gacgttgcca
     5401 gcatcgacac caatgaagag aagcgcaacg cgcacctgcg ctcggacgac ttcttcaaca
     5461 ccgagcagta cccaaccgcc accttccgca gcacgagggt cgagccggat ggcgacgatg
     5521 agttcaaggt ctatggcgac ctcacgattc gcgaccagac gcacccggtc gtccttgacg
     5581 ttgagctgga gggacaggtt ctcgacccgt acggcctcca gcgggccggg ttcgaggcca
     5641 agaccgagat cagccgcaag cagttcggcc tcaactggaa cgcgatgctc gagacgggcg
     5701 gcgccgtcgt cgccgacaag gtcaagctgc tcttccacat cgaggctacc cagcagcagg
     5761 gctagtcgag gatagatctg gcgtcggggc gatgtcgggc gaggctgcgg ccatcgcccc
     5821 tacgccggga aggttatgcg ggttgggcga cgccggcccg catcggcttg aggacgttgg
     5881 accagcgcac aagctggtcc agcatcgtaa tagcggcatc ctggatcagc tcttctggct
     5941 cgaacgcgcc atcgtcggtc atgaagcgct ggaagagcgg gacgacgacg gcttccggca
     6001 gcggcgtcag cttgagcacg ttggcgatcg gcttgatcat ctggacggcg cgggtgccgc
     6061 cggcaacgcc gccgtagctg acgaagccga gcggcttgta ctgccactcg tggttgagat
     6121 agtcgatcgc gttcttgagc ggagcagtca ttccgtggtt gtactccggc gtcacgaaga
     6181 caaaggcatc ggccgcgtcc acccgcgcac tccagtcttt cgtgtgctgg tgcgtgtact
     6241 gacgcaatcg aggatggttc ggctcgtcca tgaacggcag gttgatctct tccaggtcgg
     6301 cgtagtcgat cgcgaacgtg ccgtgcgcct cggctttggc ccggaaccac tcgccaatcg
     6361 gcccgccggc ccggcccggc cggttgctgg caaccacgat catcagtttc ggcgtcgtcg
     6421 gctcgtccat cgcatccccc tcgcggttgg cgtccacacg gctgcgttcc agcctttgcg
     6481 gggatactat gcatgtggtg tgccgatgaa ctcaacgctt ccggcacgcg tcgcactgtc
     6541 aactggccct gtttgtgcga gcgcgaggtt ctgagggtca cattcgcgca ggcgatacct
     6601 gtcgcctggt agaacatcat cgataatcca gccggcctga cccggcaagc attcggagca
     6661 acagcatgac tgacacgacc accaccaccc agccttcggt gccggccggc acgcatatcg
     6721 gccacgtcca cctcaaggtc gccgacctcg aacgggccgt cacgttctac cgggacgtga
     6781 tgggcttcga cgtcgtcgcc cgctacggta cgcaggcagc tttcctctcg gccggtggct
     6841 accatcacca catcggcctc aacacctggg agagtgccgg cggcgacccg cctccgtcgg
     6901 ggacaactgg tctctaccat ttcgcgatca actacccgac ccgtcgcgac ctggccgttg
     6961 ccttcaggcg gctgctcgag aacggctggc agatcgacgg tgcatccgac cacggcaccc
     7021 acgaggcgct ctacctgcat gatcccgact tcaacggtat cgagctcgcc tgggatcgcg
     7081 atcccgccga atggccccgc aatgccgatg gcagcctgaa catgttcacc caccgcctcg
     7141 acctccgcgc cctgctggcg gaagctgaac cggtgacggc gtaagcgctg gacgcgggaa
     7201 gcaggacgcg gagtaaacgc tcgtccg
//
LOCUS       DASUGJ010000509         2969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1747894, whole genome shotgun sequence.
ACCESSION   DASUGJ010000509 DASUGJ010000000
VERSION     DASUGJ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747894"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VFV93_11460"
     CDS             <1..948
                     /locus_tag="VFV93_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166005.1"
                     /translation="TIAGTLEDLAATGDLDIAGDLLIQGSGAASTVVDGGQLDQVFQI
                     IGTSDVTVSGLTIQDGNAAGWVDPSGNRHGPMGGAILNPAGSVTVSDSVFAGNQSPED
                     AGAIYNNGQLTIERSQLRDNQIVDFGNGGAIVNDANGVLTISDSQFEDNSTSDAGGEG
                     GAIAAFGGTVSIDGSEFDNNIADGGGAIFSDAPSLTVSNSTFRGNQALQSQSTSGGGG
                     AIYSTGSLALSNDVFDGNYSGQSGGAVYARGGLTVDTVWFANNQGVIGGALRGNGDIS
                     NVTFSHNWVEPYEGNSQGGAMSGGGTLTNVTFSDNWAIL"
     gene            complement(1123..1449)
                     /locus_tag="VFV93_11465"
     CDS             complement(1123..1449)
                     /locus_tag="VFV93_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166006.1"
                     /translation="MQGWPLGVGGGGLIGADVAGGGAIAVAVEWAGDPALVGRGAGGR
                     IAVVDRRAAAGERVGWRKAAVVREWQQQRISVLAIAGLGEVATRAALDVVAQRGDRDS
                     GATGLA"
     gene            1471..1956
                     /locus_tag="VFV93_11470"
     CDS             1471..1956
                     /locus_tag="VFV93_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166007.1"
                     /translation="MILNGSANADPNASDPSGIASASCDPVDTTTAGTHTLTCTATDT
                     AGNTATASVSYDVTYAVALRYDPQRPTRYGINVELALSLTDANGNNVSSRSIRVTAVA
                     LRDANGRTVQTLNTHFKYVKKNQSYIIKLKAKGLDKGTYQLEFRVAGDPVAHLAPFRL
                     R"
     gene            complement(2078..2905)
                     /locus_tag="VFV93_11475"
     CDS             complement(2078..2905)
                     /locus_tag="VFV93_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166008.1"
                     /translation="MARSGLVALGWRRALVSVIVVVLLGGCGVPEELPTPTPTRSVSP
                     SPTATRATPTSTAAPETATPTATATLGATPTPLSLRVTATVPPTATTSATATPSTSDR
                     TGPATTTAFAPGPVPAATVPILQPEASATAATVVTVEATIALEPTPTLTPTPEPTATP
                     RLEPEPTPTPDLCPGAIPWWDAINYIGVETTVIGPVVDTEWAEESQGKPTFLNLGLPY
                     PDPGRFTVLMWIDARWNFDFAPEEVYLGQTVCVYGTIALYEGSAEMVIEGPEWIWAP"
BASE COUNT          539 a         1005 c          951 g          474 t
ORIGIN      
        1 acgatcgccg gtacgctgga agatctcgcg gcgaccggcg acctcgacat cgccggcgat
       61 ctgctcatcc agggatcggg tgcagcgtcc acagtggtcg atggtgggca gctcgatcag
      121 gtgtttcaga tcatcggaac gagtgatgtg accgtctccg gcctgacgat ccaggacggg
      181 aacgcggccg gctgggtcga tccatccggg aatcggcatg ggccgatggg tggcgccatt
      241 ctcaacccgg ccgggtcggt cacggtcagc gacagcgtct tcgccggcaa ccagtcgccg
      301 gaagacgcgg gagccatcta caacaacgga caactgacta tcgaacgttc gcagttacgc
      361 gacaaccaga tcgtcgattt cggcaacggc ggcgcgatcg tgaatgacgc gaacggcgtg
      421 ctgaccatct cggactcgca gttcgaggac aactccacct cggacgccgg tggtgaaggc
      481 ggcgcgatcg cggcattcgg cggcaccgtc tcaatcgacg ggagcgagtt cgacaacaac
      541 atcgctgatg gtggaggtgc catcttcagc gacgcaccgt cgctgaccgt ctccaattcg
      601 acctttcggg gcaaccaggc gctgcaatcg cagagcacca gcggtggcgg cggggcgatc
      661 tactcgaccg gcagtctcgc gttgtcgaac gacgtcttcg acggcaacta ttcaggccag
      721 tcgggcgggg cggtctacgc gcgcggcggg ttgaccgtcg acaccgtctg gttcgccaac
      781 aaccagggtg tcatcggcgg ggccttgcgg ggcaacggcg atatcagcaa cgtcacgttc
      841 agccacaatt gggtcgagcc atacgagggc aactcacagg gcggtgccat gtccggcggc
      901 gggacgctga cgaacgtcac cttcagcgac aactgggcca tcctgtagaa tcccgacgat
      961 gcgttcaacc agggcggtgc gttctacggc gatggtacct tcatcaacgt caccttcgcc
     1021 ggaaacagcg cctacgccga cgacggccag accgccctcg acggcggcag catctatgcc
     1081 aatggagaac tacaggtcgt caacacgatc gtcagtgaca catcaggcca ggccggttgc
     1141 gccggagtcg cgatcacctc gctgggccac aacatcgaga gcggcgcgag ttgcgacttc
     1201 acccagccca gcgatcgcca gaacactgat ccgctgctgc tgccactcgc ggacaacggc
     1261 ggcctttcgc caacccacgc gctcgccagc ggcagcccgg cgatcgacaa cggcgatcct
     1321 gcctcctgcc ccgcgaccga cgagcgcggg gtcccccgcc cactcgacgg cgacggcgat
     1381 ggcaccgcca cctgcgacat cggcgcctat gaggccgccc ccgccgacac cgaggggcca
     1441 accctgcaac cggcagtcat acccaacccg gtgatcctga acggcagcgc caacgccgac
     1501 ccgaacgcca gcgacccctc cggcattgcc tccgcgtcct gcgacccggt cgacacgacg
     1561 accgccggca cgcataccct cacctgcacg gcgacggaca ccgccgggaa caccgccacc
     1621 gccagcgtca gctacgacgt gacctacgcc gttgcgctcc gctacgatcc gcagcgccct
     1681 acccggtacg gcatcaacgt cgagctggcc ctctcgctga cggacgccaa cggcaacaac
     1741 gtctcatccc gatcaatccg cgtcaccgcc gtcgcgctcc gtgacgccaa cggccgaacg
     1801 gtccagaccc ttaacacgca tttcaagtac gtcaagaaga accagagcta catcatcaag
     1861 ctgaaggcca aagggctgga caagggaacc tatcaactgg agttccgtgt cgccggtgat
     1921 ccggtggcgc atctcgctcc gttccggctg agatagatgc ttcccacccg gtaggggcga
     1981 ggcactgacg cgcccttgct ccccgcccga acagcttggt ggggaaacga agggcgagtc
     2041 ggtgcctcgc ccctaccgga tacgtttccg ggggaggtta cggcgcccag atccactcgg
     2101 ggccctcgat gaccatctcg gcgctgcctt cgtagagggc gatcgtgccg taaacgcaga
     2161 cggtctggcc gaggtagacc tcctcgggag cgaagtcgaa gttccagcgc gcgtcgatcc
     2221 acatcaggac agtgaagcgg ccgggatcgg ggtagggcag gccgagattc aagaaggtcg
     2281 gcttgccctg actctcctcg gcccattccg tgtcgacgac cgggccgatc accgtcgttt
     2341 cgacgccgat gtagttgatc gcgtcccacc acgggatcgc gcctgggcag aggtccggcg
     2401 ttggtgtcgg ttcgggttcc agtctcgggg ttgccgtcgg ctcaggtgtc ggcgttaggg
     2461 tcggcgtcgg ctcgagtgcg atcgtggctt cgacggtcac aacggtggca gccgtcgctg
     2521 acgcttccgg ctgaaggatg gggaccgtcg cggccggcac aggaccgggc gcgaatgccg
     2581 tcgtcgtggc tggccccgtc cggtcgctgg ttgagggtgt cgccgtcgcc gaagtcgtcg
     2641 cggttggcgg gactgtcgcc gtcactcgca ggctcaacgg cgtcggcgtt gctccgagcg
     2701 ttgctgtcgc cgtcggtgtc gcggtctcag gagcggcggt gctggttggc gttgcccggg
     2761 tcgcggtcgg ggatggtgac acggaacgtg tcggtgtcgg cgtcggcaat tcctccggga
     2821 cgccgcagcc tccgaggagt acaaccacga tcacagaaac gagcgcccgg cgccagccga
     2881 gcgcaaccag acctgagcgt gccaccttgc cgcaccccct ggcgcttcac acatctcttt
     2941 cgtcacgaca tcctagcacg gatacacac
//
LOCUS       DASUGJ010000510         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1191982, whole genome shotgun sequence.
ACCESSION   DASUGJ010000510 DASUGJ010000000
VERSION     DASUGJ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191982"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VFV93_11480"
     CDS             complement(<1..1246)
                     /locus_tag="VFV93_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5166009.1"
                     /translation="MADTEYNLRDRLPNFTLSRRLFLGAMAGTFAASLLAACGGDDDD
                     DDDDAPTSTSGTSGQTSTETTSTETTSTEPTEAAAGETASATEDGEGEEVRGTDVKEF
                     DNPPAGTPEGNKPDELILAWGTSQFQTHGIDPQLHVGTIAETQLRHMYEPLVKFERDL
                     TTISPCLATAWERVDDLTVQFQLREGVTFHNGEPFNAEAVAYSVMRPLSDETPGDARS
                     TYSIIEGVDVVDDMTVNVKTSKPDPALLARMTGFHMVIVAPEWAKGGPEVVSKEANGT
                     GPYKFVSWTPNEDLVVEANEDYWGDVPSIKKVRMTTIVEQATRVAALRAGDVHVAKDM
                     PPEELDAINQTSRSRAVRAVSNRVPFYFITTEVEPFDNPLVRQAINYAANVDGVIEAI
                     VLGNANRVSTIIGPWIFGFDPTL"
     gene            complement(1340..2389)
                     /locus_tag="VFV93_11485"
     CDS             complement(1340..2389)
                     /locus_tag="VFV93_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5166010.1"
                     /translation="MTATPSTQAISREELAERVGKLPRVRIAHLPTPLEEAARLRAAL
                     GPDAPRILIKRDDATGLALGGNKVRHLEFRLGDIQAKGADTLIVTNVAQSNHARLHTA
                     VAARFGLTNYIIKIPSQKDDPVNGNLLLDHIMGARIIDAPSAEPEVIERELTDLVAQL
                     EAEGHVVYNVPKDSFSKIAGACAYLLGANELLGQLDERGLKADHIFLASGTSSAGLAL
                     AGKLLGETYRVHPVSVGPSRAEIEAYVCAAANGIAELLGLDARLNANDISVHDEYAGE
                     AYGIPTEAGMAALRLVGRSEGLILDPVYTSKAMAALVDQVSQGTIESNATVVFIHTGG
                     LPITFAYNEEVMRSI"
BASE COUNT          360 a          804 c          775 g          513 t
ORIGIN      
        1 ccagcgtcgg gtcgaagccg aagatccacg gcccgatgat cgtcgagacg cggttcgcgt
       61 tcccgagcac gatcgcttcg atgacgccat cgacgtttgc cgcgtagttg atcgcctggc
      121 gcacgagcgg gttgtcgaac ggctcgacct cagtcgtgat gaagtagaac gggacgcggt
      181 tactgacagc ccggacggcc cgcgaccggc tggtctggtt gatcgcgtca agctcttccg
      241 gcggcatgtc cttggcgaca tgcacatcac cggcccgcag ggccgccacg cgggtcgcct
      301 gctcgacgat cgtcgtcatc cggaccttct tgatgctggg gacgtcgccc cagtagtcct
      361 cgttggcctc gacgaccaga tcctcgttcg gcgtccacga gacgaacttg tacggcccgg
      421 tcccgttggc ctccttcgag acgacttcgg gtccgccctt ggcccattcc ggcgcgacga
      481 taaccatgtg gaagccggtc atccgggcca gcagggctgg gtccggcttg ctggtcttga
      541 cgttgacggt catgtcatcg acgacgtcaa cgccctcgat gatgctgtag gtgctgcgcg
      601 cgtcgcccgg cgtctcgtcg ctcagcgggc gcatgacgct gtaggcgacc gcttcagcgt
      661 tgaacggctc cccgttgtgg aatgtcacac cctcgcgcag ctggaactgg acggtcaggt
      721 cgtcgacccg ctcccaggct gtcgccaggc agggcgagat cgtcgtcagg tcgcgctcga
      781 acttgaccag cggctcgtac atatggcgca gctgcgtctc ggcgatcgta ccgacgtgca
      841 gttgcgggtc gatgccgtgt gtctggaatt gcgacgtgcc ccaggcaagg atcaactcgt
      901 ccggcttgtt gccttccggc gtgccggcag gcgggttgtc gaactccttg acatccgtcc
      961 cgcggacctc ttcgccttcg ccatcttcag tcgcagacgc cgtttctccg gcagccgctt
     1021 cggttggctc ggtgctggtg gtttcagtgc tggtcgtttc ggtcgaggtc tggccggacg
     1081 tgccgctggt tgaggttggc gcatcgtcat cgtcgtcatc gtcgtcaccg ccgcaggccg
     1141 ccagcaatga tgcggcgaac gtgccggcca tggcgcccag gaagagccgt ctgctcagcg
     1201 taaagttggg cagtctgtct cgtaggttgt actccgtgtc agccatgcga gttcctcctc
     1261 attacgacgt tgcgatcgat gctcactctc ccccgctgat ccggaccgac accctccttt
     1321 caggacatgc agcctgacgc tagatgctgc gcataacctc ctcgttgtac gcgaaggtaa
     1381 tgggaagccc gcccgtgtgg atgaagacga cggtggcgtt gctttcgatc gtgccctgcg
     1441 atacctgatc tacaagcgct gccatcgctt tggacgtgta gaccggatcg aggatcagcc
     1501 cctcggaccg gcccaccagt cggagcgcag ccatgcccgc ctcggttggg atgccgtatg
     1561 cctcgccggc gtattcgtcg tggacgctga tgtcgttggc gttgaggcgc gcgtcaaggc
     1621 ccagcaattc cgcgatgcca ttcgccgccg cgcagacgta ggcttcgatc tcggcccggc
     1681 tcggcccgac actgaccgga tgcacccgat acgtctcgcc aagcagcttt ccggccagtg
     1741 ccagccccgc cgacgacgtt cccgatgcca ggaagatgtg gtccgccttc aggccccgtt
     1801 catcgagctg gccgagcagc tcatttgcgc ccaacaggta ggcgcaggca ccggcgatct
     1861 tcgaaaatga gtccttcggc acgttgtaga cgacgtgccc ctctgcctcc aactgggcaa
     1921 cgagatccgt tagttcccgc tcgatgacct cgggttcggc gctcggcgca tcgatgattc
     1981 ttgcgcccat gatgtgatcc agcagcaggt tgccgttgac cgggtcgtcc ttctggctgg
     2041 gaatcttgat gatgtagttc gtcagcccga atctggccgc gacggctgtg tgcagccggg
     2101 cgtggttcga ctgggcgacg ttggtgacga tcaacgtgtc agcgcctttt gcctggatgt
     2161 caccgagccg gaactcaagg tggcgcacct tgttgccgcc cagcgccagc ccggtcgcat
     2221 cgtcacgctt gatcaggatc cgtggcgcgt ccggcccgag tgccgcgcgc agccgtgccg
     2281 cctcttcgag cggcgtcggc aggtgcgcga tccgtacgcg cggcagcttg ccgacccgct
     2341 ccgcgagttc ctcgcggctg atcgcctgcg tcgatggtgt tgctgtcatt cctcgccccc
     2401 tcgtccgtcg tcaccactcg accggtgcga ttcggttgtg gatgttcagc ta
//
LOCUS       DASUGJ010000511        10732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_220706, whole genome shotgun sequence.
ACCESSION   DASUGJ010000511 DASUGJ010000000
VERSION     DASUGJ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10732
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220706"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFV93_11490"
     CDS             complement(<1..674)
                     /locus_tag="VFV93_11490"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166011.1"
                     /translation="MTVQRDAAQDALIQIEKVLRLEERQGFRDASATIGLARFVGERT
                     ARLLGASDAATRTQVQELDALLADYATLLTGERARSVSAARSIVAGLLQRSPNGRTPV
                     APPQRQVATPRPARQAPASASTVSPSATPRPVAAATQARPAPAKRPTGRISSLSDSVR
                     LLPGVGEARVKLLEQLGVRTVRDLLRLYPRRHIDYSNVQKIGSLLFGHISTNQGTVQS
                     IESSRTR"
     gene            813..2537
                     /locus_tag="VFV93_11495"
     CDS             813..2537
                     /locus_tag="VFV93_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5166012.1"
                     /translation="MTERGAGGQRGLGWLTGRTERIDIPEERSDEVREMAKLTRDFVT
                     REVVPVIERMEHGELELNATLMRKLGELGPLAVEVPEEYGGLELPKAVGTAVVEELAP
                     TGGFAVTYAAHTTIGTLPLVYFGTDEQKRRYLPRLASGEWVGAYALTEPGSGSDALNA
                     RTRATLSKDGTHYILNGTKQFITNSAFAGLFTVFAKVDGEHFTGFLVEPDFPGVSLGD
                     EEHKMGIKSSSTRQLILQDAQVPVENLLGDVGKGHRIALNVLNIARFKLGVAGIGGSK
                     GALALSARYAQEREQFGKQIASFGMIRQKLAEMATHTYAAESAAYRLVGQIDRAIEGK
                     TGTTAVLSGIQEYAAECAILKVLGTETLDYCVDEGVQIHGGYGFLQDYPIERAYRDSR
                     INRLFEGTNEINRLLAPSTLIRKAAKGELALSELAAQTPPADGNIEQQQIDDLKHLTL
                     LLASLTLDRFGKNAEEEQEVLATIGDILIETYAAESAVLRAEQTGNPLWHSMARIYLD
                     VTLDRARTRANTIAPRITSDVTGTIQRIAQLTTREPRDLIAVQREIADSVLAANGYPA
                     AVYASVTA"
     gene            2675..3460
                     /locus_tag="VFV93_11500"
     CDS             2675..3460
                     /locus_tag="VFV93_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166013.1"
                     /translation="MKRFLRVLGAVPYSPFRQFIIGLLGLTLAFVVAAVALYLAAVEP
                     VWWILPLCFAGSCLFSLGLIALLSRPYVRDIDTIRQNPLVHWTYAADEWARFEEAQWK
                     RETGSVRSYPLFTAIIALVVAGLAYVGSRDLLVTLGAFGLFVAIGALVTLQTWGMAKW
                     RYARRAKAAGDVYISTSGILQPQGYLPYSGFNVRLTEAEVVPGDPDTLRLQISSYSEN
                     AISRTSDIRIPVPYGREDEAREVAQQLLGASAARGYKPAVPVR"
     gene            complement(3577..3753)
                     /locus_tag="VFV93_11505"
     CDS             complement(3577..3753)
                     /locus_tag="VFV93_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166014.1"
                     /translation="MSATACAKCGSKRITEIAAMEDRPASIWCHACGAVLTKPIEPDQ
                     ASVGDRLLVSALND"
     gene            complement(3895..5598)
                     /gene="ggt"
                     /locus_tag="VFV93_11510"
     CDS             complement(3895..5598)
                     /gene="ggt"
                     /locus_tag="VFV93_11510"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5166015.1"
                     /translation="MTVTEPSRVTQHASRVTTIKQDATASKGMVTSNHPLASLAGTEM
                     LVMGGNAIDAAVATMFALSVVEPMMTSIFGAGFLNIRLADGTCTTIDNYATVPGGARA
                     DMFEPIPDNLENDVVGALNTTGYLAVATPGTLLGWATAVEQYGRLSLAEVLQPAIRFA
                     RHGYRVSPYLNMIIDMERDNLGRFPASADVFLPGGSVAPVDSTLIRRDYADTLEAIAA
                     QGPDYLYRGELGRVVAEDMAKNGGLITEEDLAGYRVYERAPVRGTYRGYEIVSMGPTS
                     SGGTHIIQILNILEGFDIAGMGFGTADTVHVIAEAMKIAFADRFRYMADPATTDIPLD
                     WLTSKAYGDERRGHIDMRRAQAYTAALRPEGDGASTTHCCAMDAEGNVVSTTQTLNGG
                     FGSKVTVPGTGMLLNNCMHLMDPNPGRTNSIAPGKRILSSMSPTIVLKDGQPFMALGT
                     PGGVRIFGAVMQAIVNVIDHGMTLQQAVEAPRLWDRGPTLEIEQGFADLAGLKVELER
                     RGHQVETPFKVAGGMNGIMRDAETGLLRGAACWRADGAPMGFSGGDAIVEGDVANAMW
                     A"
     gene            complement(5668..7266)
                     /locus_tag="VFV93_11515"
     CDS             complement(5668..7266)
                     /locus_tag="VFV93_11515"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5166016.1"
                     /translation="MLETLLITNGTILTMDPAQPEVEAVGVIGETIIAAGSRQAVEAA
                     LPAGYKTLDLAGQTMLPGFNEAHNHMIGYGTALGQIDTGFPAVTSIADIVAAVRERAA
                     STPAGQWIIGRGYDDNKLEERRHPNRWDLDAATTQHPVLIVNGSGHLSAANSRALELA
                     NITRDTPDPVGGHFVRDEHGEATGVLHETAQQPVRENIPAPTVEDHVAALQRCNDAYV
                     ASGITSSQDAGSSMPEHLQAYQIAVERGLLKLRTSMMIRENLLPHIVGLGVQQGLGSD
                     RLRIGPIKMFIDGSLIGRTAAVTIPFENDPRDDNLGLTMMPKEQFEDYVMQAHTAGFQ
                     IAVHAIGDRGIDWVLDAYEKALTARPRADHRHRIEHCGICRPDIIERIKRLGVIPVSQ
                     PVFIIEYGDGFIRHLGIERVQLTYPFRTFLDAGIRLVFSSDCPVSSYVPLKSVQAAVT
                     ERTGSGQSYALEEAVTVDEALEMFTVAGAYCTFEEDIKGQIKPGMLADFAVLEHDPRT
                     VDPMTLADLKVTRTIIGGETVYEA"
     gene            complement(7297..8304)
                     /locus_tag="VFV93_11520"
     CDS             complement(7297..8304)
                     /locus_tag="VFV93_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5166017.1"
                     /translation="MAATTQMTARERVLAALAGQDVDRPPVSLWQHFPERDQSAAALG
                     ESTRWWQGLLDLDFIKLMPPGDYATIDWGLTSEYRGAKGGTRDTTHYPIQSVEDWRAI
                     KPVDVGAGFNAQMVAACGLVRDILGSDVPLLQTVFSPLTIANKLSAGLVIEHLRSNPD
                     QIHETLSVIRDVTVEVAKASIAAGASGVFFASQCATTDVMTREEYDAFGVPYDLPVLE
                     AANGAGSEFTLIHIHGANTFFDLLAGYPGQAINWHDRRIGPDIPTALAGSPDRAAVAG
                     IDEHGIADMSPDDVRKQVRQAREATGDRRLLIGPGCVITVATPMENLQAAVSAAREPL
                     G"
     gene            complement(8347..10347)
                     /locus_tag="VFV93_11525"
     CDS             complement(8347..10347)
                     /locus_tag="VFV93_11525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5166018.1"
                     /translation="MSETTASATDEKKKPAPVVAEAGKPLTPERLAESKQPADPRLSP
                     DGTLIAFVLQQASKAEEHEQTAIWVVPFEGDASDGARQFTSGLWNDTSPRWSPDGRRL
                     AFFSDRAERGKMSVYLMPADGGEARRVFDEQGSMNSLAWSPDGRSLGVLYTPGETEDE
                     KKRKEERDDARVWDEEDKYQRLWVIDLDAESAAPVTPENRQAHGFAWSPDSARIAYVS
                     THHPRINDQFGENEVWIVARQGGEPVRAFAQIGEPAGLLWSNDGKSLAFLGRGDKVVH
                     PEVIFTIPVSGGEPQNITPDYWGTFEYLAPDGDGSALLAFGTEGVNQLPYRVSWDGEL
                     QPLLAGGQAGFSFRGTSIDIVGNRLVAAWEGPDAPANIYVAETLVGGGDGRLRRRTDY
                     NADLLAAALGETEVVRWQSDPGVEVEGILVKPHGYMEGMRYPLVVQVHGGPTWAWSNT
                     FNANWHDWAQMLAGRGYAVLMPNPRGSTGRGVAYNNQIFDDVGGGEYRDMIAGVDALI
                     EKGIADPDRLGVGGWSWGGYMTAWTVSQTTRFKAAVMGAGLPNMVTDNSIGDIPSAND
                     SYFSTSPYEDPEPLWERSAIRYINRVTTPTLVLHGEEDRRVNTMQGMEMYIALRQRGV
                     KTRFVTYPREGHGIRERKHQIDLMQRIIAWYDEHLRGECDEK"
BASE COUNT         1726 a         3685 c         3449 g         1872 t
ORIGIN      
        1 cgcgtccgcg acgactcgat cgactggacc gttccctggt tcgttgagat atggccgaag
       61 agcaggctgc cgatcttctg gacgttgctg tagtcgatgt gacggcgggg atagagccgc
      121 agcagatcgc gaaccgtccg caccccaagc tgctccagca gcttcacccg tgcctcgccg
      181 actcccggca gcagcctgac cgagtcggag aggctgctga tccgccccgt tggccgcttc
      241 gccggagccg gccgcgcctg agtggctgct gcgaccggac gaggcgtcgc tgagggagac
      301 accgtgcttg cggatgccgg agcctggcgt gccggacgcg gcgttgcgac ctgacgttga
      361 ggtggtgcga ccggggtacg tccgttcgga ctgcgttgca acagacccgc gacgatgctg
      421 cgtgcggcac tgacgcttct tgcccgctcg cctgtcagca gggtggcata gtcggccagc
      481 agcgcgtcaa gctcctggac ctgcgttcgc gtcgcagcat ccgacgcgcc gagcagccgc
      541 gccgttctct ctccgacgaa gcgggccagc ccaatcgtcg cgctcgcatc acggaatccc
      601 tgccgctctt cgagccgcaa caccttctcg atctggatca gcgcgtcctg cgcggcgtct
      661 cgctgcaccg tcatggccga agtgttggtc aggagcgatg gcagtgtcaa ccagccgggc
      721 gcgttgtgca gaaaccaagc gacttgtcgt gtggcacggc atcatccatc atggaagcgc
      781 acagatccgg cgatgacgga aaggaacgcg acgtgacgga gcgtggagca ggcgggcagc
      841 gtggcctcgg ctggttgacc ggccggacgg agcgaattga cattcccgaa gagcgtagcg
      901 acgaggttcg cgagatggcc aagctcaccc gcgacttcgt cacgcgcgaa gttgtgcccg
      961 tcatcgagcg gatggagcac ggcgagctgg agttgaacgc caccctgatg cgtaagctcg
     1021 gtgaactggg gccgctggcc gtcgaggtgc cggaagaata cggcgggctg gagctaccga
     1081 aggcggtcgg cactgccgtc gtcgaggaac tggcgccaac cggcggcttc gccgtcacct
     1141 acgccgccca cacgacgatc ggcacgctgc cactggtcta cttcggcacc gacgagcaga
     1201 agcggcgcta cctgccgagg ctggcatccg gcgagtgggt cggcgcctac gcactgaccg
     1261 agccgggctc cggctctgac gctctcaacg cccgtacccg cgcgacgttg tcgaaagacg
     1321 gcactcacta catcctgaac ggcaccaagc agttcatcac caactcagcg ttcgcgggac
     1381 tcttcaccgt tttcgccaag gtcgacggcg agcacttcac cggcttcctg gtcgagccgg
     1441 actttcccgg cgtctcgctc ggcgacgagg aacataagat ggggatcaag tcctcgtcga
     1501 cgcgtcaact gatcctgcag gacgctcagg tccccgtcga gaacctgctc ggcgacgtcg
     1561 gcaagggcca ccgcatcgcc ctgaacgtcc tcaacatcgc ccgcttcaag ctcggcgtgg
     1621 ccggcatcgg cggcagcaag ggcgcgctgg cgctctcggc ccgctacgcg caggaacggg
     1681 agcagttcgg gaagcaaatc gcctcgttcg ggatgatccg ccagaaactg gccgagatgg
     1741 cgacccacac ctacgccgcc gaaagcgccg cctaccggct ggtcggccag atcgaccggg
     1801 ccatcgaggg caagaccggc acgacggccg tcctctccgg cattcaggag tacgccgccg
     1861 agtgcgcgat actgaaggtg ctcgggaccg agacgctcga ctactgcgtc gatgaggggg
     1921 tccagatcca cggcggctac ggcttcctgc aggactaccc gatcgagcgc gcctaccgcg
     1981 actcacgcat caaccgcctc ttcgagggca ccaacgagat caaccgtctg ctcgcgccat
     2041 cgacgctgat ccgcaaagcc gccaagggcg agctggcgct atcggagctt gccgcccaga
     2101 caccaccggc cgatggaaac atcgaacagc agcagatcga cgacctgaag caccttacgc
     2161 tgctgctcgc cagcctgacg ctcgatcgct tcggcaagaa cgctgaagaa gagcaggagg
     2221 tactggcaac gatcggcgac atcctgatcg agacctacgc cgccgaatcg gccgtcctgc
     2281 gcgccgagca gaccggcaac ccactttggc attcgatggc ccgcatctat ctggatgtca
     2341 cactcgatcg cgccaggacg cgtgccaaca cgatcgcccc tcgcatcacg agtgacgtta
     2401 ccggcacgat ccagcgcatc gcccagctca cgacacgcga accaagggat ctgatcgccg
     2461 tccaacgtga gatcgccgac tccgtcctgg cagccaatgg ctacccagcc gccgtctacg
     2521 ccagcgtgac ggcataatag aatccacgat acaaggtagg ggccggcctc gtgccggccc
     2581 tggttccctc ccgacgcgtt cggggatcga agcagggccg gcacgagacc ggccccgact
     2641 tgtggtctgt tgggttgtga gaagacggat gaggatgaag cgctttctcc gtgtccttgg
     2701 tgccgtccca tatagcccct tcaggcagtt catcatcgga ctgctcgggt tgacgctcgc
     2761 cttcgtggtc gcggctgtcg cgctgtacct ggcggcggtg gagccggtct ggtggatcct
     2821 gccgctctgt ttcgccgggt cgtgtctctt ctcgctgggc ctcatcgcgc tactgagccg
     2881 gccgtatgtg cgcgacatcg acacgatccg ccagaacccg ctcgtccact ggacctacgc
     2941 cgccgacgag tgggcacgct ttgaggaggc acagtggaag cgggaaacgg gcagcgtccg
     3001 atcctacccg ctcttcacgg cgatcatcgc cctggtcgtc gctgggctgg catatgtcgg
     3061 ctcccgcgat ctgctggtaa cgctcggtgc gttcggcttg ttcgtcgcga tcggtgcgct
     3121 ggtgacgttg cagacgtggg gaatggcgaa gtggcgctat gcccgtcgtg ccaaggcggc
     3181 tggtgacgtc tacatcagca cgagcggcat tcttcagccg caaggctatc tgccctacag
     3241 cggcttcaat gtcaggttaa cggaggcaga ggtggtgccg ggcgacccgg acaccctccg
     3301 cctccagatc tcaagctaca gcgagaacgc catatcgcgc accagcgaca ttcgaattcc
     3361 ggtcccgtat gggagggagg acgaagcgcg cgaggtcgcg cagcagttgc ttggagcgag
     3421 cgcggcacgg gggtacaaac cggcagtgcc ggttcggtag ggcgatgtcc ccctgtcgtc
     3481 ctgagcgaca gcgaaggacc tctccgcgtc aacctcgtgt ggcgctaaag cggagaggtc
     3541 cttcgctgcg gctcaggacg acaggaggtt gcgccgctaa tcattcaacg ccgagacgag
     3601 caacctgtcg ccgacgctgg cttgatccgg ctcgatcggc ttggtcagga cggcgccgca
     3661 ggcatggcac cagatcgacg ccggccgatc ctccatcgcg gcgatttcgg tgatccgctt
     3721 gcttccacac ttcgcgcagg cagtcgcact catcggggcc gacctttccg ctctcgtccg
     3781 gcggggcgat acgcgcgtca ccccgcgttg tgatcgtaga acaggcgccg gcccgaggct
     3841 acggaccggc tgactaaccg acgcccgatg atttgggcgc gccgcacagt ccggctaggc
     3901 ccacatcgcg ttggcaacat cgccttcgac gatggcgtcg ccgccggaga agcccatcgg
     3961 cgcgccgtcc gcccgccagc aggccgcgcc acgcagcagc cccgtctcgg catcgcgcat
     4021 gatgccgttc atgccgccgg ccaccttgaa cggcgtctcg acctgatgcc cgcgccgctc
     4081 aagctcgacc ttcagcccgg ccagatcggc gaagccctgc tcgatctcca acgtcgggcc
     4141 gcgatcccac agccgcggcg cctcgaccgc ctgctggagc gtcatgccat ggtcgatgac
     4201 gttgacgatc gcctgcatga cggcgccgaa gatgcgcact ccgcccggcg tgccgagcgc
     4261 catgaacggc tggccgtcct tgaggacgat cgtcggtgac atcgacgaga gaatgcgctt
     4321 gcccggcgcg atcgaattcg tccggcccgg gttcggatcc atcaggtgca tgcagttgtt
     4381 cagcagcatc ccagttcccg gcaccgtcac cttgctgccg aagccgccgt tgagcgtctg
     4441 ggtggttgag acgacgttcc cctcggcatc catcgcacag cagtgcgtcg tcgacgcgcc
     4501 gtcgccctcc ggccgcagtg ccgccgtgta ggcctgcgcc cggcgcatgt cgatatgacc
     4561 gcgccgctcg tcgccgtatg ccttcgaggt cagccagtcg agtgggatgt cggtcgtcgc
     4621 cggatcggcc atgtagcgga accggtcagc gaaggcgatc ttcatcgctt cggcgatgac
     4681 gtggacggtg tcggccgtcc cgaagcccat cccggcgatg tcgaaccctt cgaggatgtt
     4741 caggatctgg atgatgtgcg tcccgcccga cgaggtcggc cccatcgaga cgatctcata
     4801 gccccggtac gtcccgcgta ccggcgcgcg ctcgtagaca cggtagccgg ccagatcctc
     4861 ctcggtgatc agcccgccgt ttttcgccat gtcctctgcg acgacccggc ccagttcgcc
     4921 tcgatagagg tagtcgggcc cctgtgcggc aatcgcctcc agcgtgtcgg cgtagtcgcg
     4981 gcggatgagc gtgctatcaa ccggcgcaac cgacccgccc ggcaggaaga catcggcgct
     5041 ggccgggaag cggcccaggt tgtcgcgctc catgtcgatg atcatgttca ggtacgggct
     5101 gacccggtag ccgtgccgcg cgaagcggat ggccggctgc aacacctcgg ccagcgacag
     5161 ccgcccatac tgctcgacgg ccgtcgccca gcccagcagt gtccccggcg tcgcgaccgc
     5221 cagatagccg gtcgtgttca gcgcgccgac gacgtcgttc tccaggttgt ccggaatcgg
     5281 ctcgaacatg tcggcccgtg cgccgcccgg cacggtcgcg tagttgtcga tcgtcgtgca
     5341 tgtgccatcc gccagccgga tgttgaggaa gccggccccg aagatgctcg tcatcatcgg
     5401 ctcgacgacc gacagcgcga acatcgttgc gactgccgca tcgatcgcgt tcccgcccat
     5461 caccagcatc tcggtccccg ccagagatgc caacggatgg ttcgatgtca ccatgccttt
     5521 cgaggcggtt gcgtcctgct tgatggtcgt gacgcgtgac gcgtgctgcg tgacgcggct
     5581 tggctcagtc actgtcatcg tttcctcact cttcatcact ccggtcgggc caaacccgcc
     5641 acccctgtca tcccgcaagc tctgctttca cgcctcatat accgtctctc caccgatgat
     5701 cgttcgcgtc actttcagat cagccagcgt catcgggtcg acggtgcgcg ggtcgtgttc
     5761 gaggacggcg aagtcggcca gcatgccggg cttgatctgg cccttgatgt cctcctcgaa
     5821 ggtgcagtag gcgccggcga cggtgaacat ctccagcgct tcgtcgacgg taacagcttc
     5881 ctccagcgcg tatgactggc cggagccggt tcgctcggtg acggccgcct ggaccgactt
     5941 gagcggaaca tagctggaga ccgggcagtc ggacgagaag acgagccgga taccggcatc
     6001 gaggaacgtc cggaacgggt aggtgagctg cacccgctcg atgccgaggt ggcggatgaa
     6061 gccgtcgccg tactcgatga tgaagacggg ctgcgagacg ggtatgacac caagccgctt
     6121 gatccgctcg atgatgtcgg gccggcagat gccgcagtgc tcgatccggt gccggtggtc
     6181 ggcgcgggga cgggcagtca gcgccttctc ataggcgtcc aggacccagt cgatgccccg
     6241 gtcgccgatg gcgtggacgg cgatctggaa tccggccgtg tgcgcctgca tgacgtagtc
     6301 ctcgaactgc tctttcggca tcatcgtcag gccgaggttg tcgtcacgcg ggtcgttctc
     6361 gaacggaatc gtgacggcgg ccgttcgccc gatcagcgaa ccgtcgatga acatcttgat
     6421 cggtccgatc cggagccggt cgctgcccag cccctgctgg acaccgagcc caacgatgtg
     6481 cggcagcaga ttctcgcgga tcatcatgct ggtccgcagc ttcagcagcc cccgttcgac
     6541 ggcgatctgg tacgcctgca gatgctccgg catcgacgag cctgcatcct gcgagcttgt
     6601 gatgccgcta gcgacgtagg cgtcgttgca gcgttgcagg gccgcgacgt ggtcctcgac
     6661 ggtcggcgcc gggatgttct cgcggaccgg ctgctgggcc gtctcgtgca gaacgccagt
     6721 cgcctcgccg tgctcatcgc gcacgaagtg accgccgacc gggtcgggcg tgtcgcgggt
     6781 gatgttggcc agctccagcg cccgcgagtt ggctgccgag aggtggcccg atccgttgac
     6841 gatcaggacc ggatgctggg tcgtcgccgc gtcgaggtcc cagcgattcg ggtggcgacg
     6901 ctcttccagc ttgttgtcgt cgtagccacg cccaatgatc cactggccgg ccggcgtgct
     6961 cgccgcccgc tcgcggacgg ccgcgacgat gtcggcgatc gacgtgacgg ccgggaagcc
     7021 ggtgtcaatc tgccccagcg ccgtcccgta gccgatcatg tggttgtgag cctcgttgaa
     7081 gcccggcagc atcgtctggc cggccagatc gagcgtcttg tagccggccg gcaaggcagc
     7141 ctcgaccgcc tggcggctgc cggccgcgat gattgtctcg ccgatcacgc cgacggcttc
     7201 gacctccggc tgggcagggt ccattgtcag gatcgtcccg ttggtaatca gcagtgtttc
     7261 cagcatctca ccaccccttc gactgacctg gttcgattag cccaacggct cacgcgccgc
     7321 cgagacggca gcttgcaggt tctccatcgg cgttgcaacg gtaatgacgc agcccggccc
     7381 gatcagcagc cgccggtcgc cggtcgcttc ccgcgcctgc cggacctgct tgcgaacgtc
     7441 gtccggcgac atgtcggcga tgccgtgctc gtcgatcccg gcgacggcgg cgcgatccgg
     7501 cgagcccgcc agcgcggtcg ggatgtccgg cccgatccgc cgatcgtgcc agttgatcgc
     7561 ttgccccgga taaccagcca gcaggtcgaa gaacgtgttc gcgccgtgga tgtggatcag
     7621 cgtgaactcg ctgccggccc cgttggcggc ctccaggacc ggcaggtcgt acgggacgcc
     7681 gaacgcgtcg tactcctcgc gggtcatcac gtcggtcgtc gcgcactggc tggcgaagaa
     7741 gacgcccgac gcgccggccg cgatcgacgc cttcgcgacc tcgaccgtca cgtcgcggat
     7801 gacgctcagc gtctcgtgga tctggtcggg gttcgagcgc agatgctcga tcaccagtcc
     7861 agccgagagc ttattcgcga tcgtcagcgg gctgaagacg gtctgcagca gcggcacgtc
     7921 cgaaccgagg atatcccgca ccagcccgca ggcggcgacc atctgggcgt tgaagcccgc
     7981 cccgacatcg accggcttga tggcgcgcca atcctcgacc gactggatcg ggtagtgggt
     8041 cgtgtcgcgg gtgcccccct tcgcgccgcg gtactcgctg gtcagccccc agtcgatcgt
     8101 cgcgtagtcg ccaggcggca tcagcttgat aaagtcgagg tccagcaacc cctgccacca
     8161 gcgcgtcgat tcccccagcg ccgccgccga ctggtcccgc tccgggaaat gctgccagag
     8221 cgaaacgggc ggccggtcaa cgtcctggcc ggccagcgcc gccagtaccc gctcgcgggc
     8281 cgtcatctgt gtcgtggctg ccatcgtggc tcccttcctt gcgtcgtcgc cgcccatgga
     8341 agtacgtcac ttttcgtcgc actccccacg caggtgctcg tcgtaccagg cgatgatgcg
     8401 ctgcatcagg tcgatctggt gcttgcgctc gcggatcccg tgcccctcgc ggggataggt
     8461 cacgaagcgg gtcttgacgc cgcgctggcg cagggcgatg tacatctcca tgccctgcat
     8521 cgtgttgacg cgccggtcct cctcgccgtg cagcaccaac gtcggcgtcg taacgcggtt
     8581 gatgtaacgg atcgccgagc gttcccagag aggttccggg tcctcgtacg gcgacgtcga
     8641 gaagtaggag tcgttcgcgc tggggatatc gccgatcgag ttgtcggtaa ccatgttcgg
     8701 cagcccagcg cccatgacgg ccgccttgaa gcgcgtcgtc tggctgacgg tccaggccgt
     8761 catgtagccg ccccagctcc agccgccgac cccgagtcgg tcgggatcgg cgatgccctt
     8821 ctcgatcagc gcgtcaacac cggcgatcat gtcgcggtac tcaccgccgc ctacgtcgtc
     8881 gaagatctgg ttgttgtagg ccacgccccg gccggtgctg ccgcgtggat ttggcatcag
     8941 gacggcgtag ccgcgcccgg ccagcatctg cgcccagtcg tgccagttgg cattaaacgt
     9001 gttcgaccag gcccaggtcg gcccgccatg cacctgaacg accagcgggt agcgcatccc
     9061 ttccatgtac ccgtgcggct tgacgagaat gccctcgacc tcgacgccgg gatcgctctg
     9121 ccagcgcacg acctccgtct cacccagcgc cgccgccagc aggtcggcgt tgtagtcggt
     9181 ccgccgccgc agccggccgt cgccgccacc gaccagcgtc tcggcgacat agatgttggc
     9241 cggcgcgtcc ggcccctccc aggcggccac cagccggttg ccgacgatgt cgatcgacgt
     9301 gccacggaag gagaagcccg cctgcccgcc cgccagcagc ggttgcagct cgccatccca
     9361 actgacgcgg tagggcaact gattgacgcc ctcggttcca aaggcgagca gcgcgctgcc
     9421 gtcgccatcc ggtgccaggt actcgaacgt tccccagtag tcgggcgtga tgttctgcgg
     9481 ctcgccgccc gagaccggaa tcgtgaagat cacctcggga tggacgacct tgtcgccccg
     9541 gccgagaaag gccagcgatt tcccgtcgtt cgaccagagc agcccggccg gctcgccgat
     9601 ctgcgcgaac gcgcgaaccg gctcgccacc ctggcgggca acgatccata cctcgttctc
     9661 gccgaactgg tcgttgatac gtggatggtg ggtcgagacg taggcgatcc gcgcgctgtc
     9721 gggcgaccag gcgaagccgt gcgcctgccg gttctccggc gtcaccggcg cggccgactc
     9781 ggcgtcgagg tcgatcaccc agaggcgttg gtacttgtcc tcctcgtccc agacccgggc
     9841 gtcgtcgcgc tcttccttgc gcttcttctc gtcctcagtc tcgcctggtg tgtagagcac
     9901 acccagcgag cggccatcag gcgaccaggc gaggctgttc atgctgccct gctcgtcgaa
     9961 gaccctccgc gcctcgccac catcggccgg catgaggtag acactcatct tcccgcgctc
    10021 ggcccggtcg gagaagaacg ccaaccggcg gccgtccggt gaccagcgcg gcgacgtgtc
    10081 gttccagagt ccggacgtga actgacgggc gccgtcggac gcatctccct cgaacggcac
    10141 gacccagatc gccgtctgct cgtgctcctc ggccttgctg gcttgctgga ggacgaaggc
    10201 gatcaacgtt ccatccggtg ataggcgcgg gtcggccggt tgcttgctct cggccagccg
    10261 ctcgggcgtc aacggtttgc cggcctcggc cacaaccggg gccggcttct tcttctcgtc
    10321 agtcgcgctg gctgttgtct cgctcacata ccctctgttt cttcaacgtc gtgagtccgt
    10381 accgccggta tcgtcgccga ttcgggcccg cgcggcaact ggcgggtcat ccgcgcccga
    10441 tcaacagcgg agaaccgtgc caacatcctt gatcggcagg accaaccacg cggcaccgat
    10501 tggctaccgc cagtcaggac ttccgggccg tgtccggcag gcatcgcttt gcaacaatga
    10561 aatcaatgac cagttgaatc gaggatcgga gcgaacggca attgtcctac cgcgctcaat
    10621 gccctgaagg cacctgtgtc ccgtcccgct cgggtggctt ctgcctgctc tgcggccggg
    10681 ccctccgctc cgcacacccg accgacgacg agctccaggc agccctgagc ca
//
LOCUS       DASUGJ010000512         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1886420, whole genome shotgun sequence.
ACCESSION   DASUGJ010000512 DASUGJ010000000
VERSION     DASUGJ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886420"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1086)
                     /locus_tag="VFV93_11530"
     CDS             complement(67..1086)
                     /locus_tag="VFV93_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5166019.1"
                     /translation="MRLLRPVFVIRLAITLALTLGVLAMSSTPSFSYPGFAKDNHGHN
                     GKSENRGKGHKDKNKGKHEDNGKHKGKDKAKKDKKDKRVKAEPLAAYTVHAECTYEPA
                     ADQTTCDFDADSPAGGKKINLLDLPSDEICAPVVGGDARYVDPDPNTGATGYRSTDPD
                     GKFTLVFSGRVTAGGTATYWIKAASDVFPATGTGLSCESGEATVPQTTTPPAAVPTAT
                     AAAVTPEVTDTTGSVVVQAYTCPIAAPPAEYDWYGQCSPVPAGSIYRLMRLDSATRDG
                     ITTSTDGAGRATFRSLPPGTYQLTQADGDWCHAQSDDVTADGNLFVKAGERTSVWVFT
                     CTGAP"
     gene            complement(1258..>2212)
                     /locus_tag="VFV93_11535"
     CDS             complement(1258..>2212)
                     /locus_tag="VFV93_11535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166020.1"
                     /translation="IGLQFWQLLILVLLGSLLDIPGVTARRALLPELAELGDVRLERV
                     NAMLEGNWQVSILLGPPIAGVLIGILGAANVLWIDAASSLISFTAILLLVPANVHAHI
                     SAVSQGYIRDLKIGLHFLWHDQVLRWISLTLAISNAFGAPFFSLIIAVYIKERYDDPR
                     YLGILLSVTGIGLLVGTTLYGAVGYRWTRAHIMRVALLMFTMSYWPFGFDLPFPVILI
                     CVAIGGLADGPVNPLLVTVRLERIPVELRGRVFAATSAMAQMFPAFTIPLAGWMIERI
                     DLTPTVLILASGAQIANLLLARLPVWDRLDETANREVAVPV"
BASE COUNT          416 a          654 c          736 g          406 t
ORIGIN      
        1 gaaatgaacg acagcggcac gatgcttcgt gccgctgtca tgccgaacta gttagccgtt
       61 acctggctat ggcgcgccgg tgcaggtgaa tacccagact gatgttcgct caccggcttt
      121 gacgaacaga ttgccatcgg cggtgacgtc atccgactgc gcgtgacacc aatcgccatc
      181 ggcctgcgtg agctggtagg taccgggtgg gagcgatcgg aatgtggccc gtcccgcgcc
      241 gtcggtcgaa gtcgtgatgc cgtcgcgcgt tgcactgtcc agacgcatca gacgatatat
      301 cgaaccggcg ggaaccgggc tgcactggcc gtaccagtcg tattcggccg gtggcgccgc
      361 gatcggacag gtgtaggcct gaacgacgac tgagccggtg gtatcggtta cttccggcgt
      421 gaccgccgcc gctgtggcgg tcgggacggc tgcaggtggg gtcgtcgttt gcgggaccgt
      481 cgcctctcct gactcgcagc tcaacccggt accggtcgct ggaaagacgt cgctggcggc
      541 cttgatccaa taggtcgcgg tgccgccggc agtcacgcgg cccgagaaga cgagcgtgaa
      601 cttgccgtcc ggatcagtcg atcgatatcc ggtcgcgccg gtattcgggt cggggtcgac
      661 atagcgggca tctccaccga cgaccggggc gcaaatttcg tcggacggca ggtcgaggag
      721 attgatcttc ttcccgcccg ccgggctgtc ggcatcgaag tcacaggtcg tctgatccgc
      781 tgccggctca tatgtgcatt cggcgtggac cgtgtaggca gcgagtggtt cggctttaac
      841 tcgtttgtcc ttcttgtcct tcttggcctt gtctttcccc ttgtgcttcc cgttgtcctc
      901 gtgcttaccc ttgttcttgt ccttgtgacc cttgccgcgg ttctcggact tgccgttgtg
      961 gccgtgattg tccttcgcga aaccggggta cgagaacgac ggtgtcgagg acatagcgag
     1021 cacgccaagc gtcaacgcca gcgtgatagc gagcctgatc acaaatacgg gacgaagcaa
     1081 cctcatagag ctctcctgat gcgccgtggt ccacggtaat cggcggcgtc gccggtcagg
     1141 gaccgcgaaa cgaactcgac cttactatgt gccgcggctg accgctgtag cgcatgactc
     1201 ggattcggga cgatcggtgg tcgaacgagc gcgtgtcgac tacacgtgcg cgttggatca
     1261 aacgggaacc gcgacctccc ggtttgcggt ttcgtcgagt cgatcccaga ccgggagccg
     1321 ggccagcagg aggttggcga tctgggcgcc actggcaagg atcaagacgg ttggagtgag
     1381 atcgatccgc tcaatcatcc agccggcgag tgggatggtg aatgccggga acatctgggc
     1441 catcgccgag gtcgcggcga agacccggcc tcggagctcg accggaattc gctcgagccg
     1501 caccgtgacc aggagcggat tcactggacc atcagccagt ccgccaattg cgacgcaaat
     1561 caatatgacc gggaagggga ggtcgaaccc gaacggccag tagctcatcg tgaacatcag
     1621 cagcgcaacg cgcatgatat gtgcccgggt ccagcgatac ccgactgcgc cgtagagggt
     1681 ggtgcccacg agcaggccga tgccggtgac cgagagaagg attcccaggt agcggggatc
     1741 gtcgtagcgc tccttgatgt agaccgcaat gatcagcgag aagaacgggg cgccgaacgc
     1801 gttcgaaatt gccagcgtca gggagatcca gcgaagcacc tggtcgtgcc agaggaagtg
     1861 cagccctatt ttcaggtcgc ggatgtaccc ctgtgagaca gcgctgatgt gcgcatgcac
     1921 gttggccggg accagcaaga ggatggcggt gaaggagatc agcgacgagg cggcgtcgat
     1981 ccagagcacg ttggccgcgc cgaggatgcc gatcaacacg ccggctattg gcgggccgag
     2041 caggatcgag acctgccagt tgccctccag catcgcattg acgcgctcca gccgcacgtc
     2101 gcccaactca gccagttccg ggaggagcgc gcgccgagcg gtgacgcccg gaatgtcgag
     2161 gagcgatccc agtagcacga gaatcaggag ctgccagaac tgcaggccga tc
//
LOCUS       DASUGJ010000513         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_80255, whole genome shotgun sequence.
ACCESSION   DASUGJ010000513 DASUGJ010000000
VERSION     DASUGJ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80255"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /gene="gcvPA"
                     /locus_tag="VFV93_11540"
     CDS             <1..1137
                     /gene="gcvPA"
                     /locus_tag="VFV93_11540"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX5166021.1"
                     /translation="FLGAGSYTHYVPAAVQQITFRGEFYTAYTPYQPEVAQGTLQAIY
                     EYQSMICALTGMEVSNASMYDGATALAEGALLTVSLPRKRTRIVMAGTIHPNYAGVVR
                     TYTSGVGVEVVQLPLPADGLKTDPAAFDEYLDDNLACIVVQYPNFFGTIEDIAAIGEK
                     AHSCGANLIVSTYPTALGLLKSPGELGADVVTGEGQPLGNAQSFGGPVVGILATHQRL
                     VRQMPGRLAGIAYDSEGKRGFVLALQTREQHIRREKATSNICTNQGLMALAASVYLAT
                     LGPQGLRRIAEICYQNGHYLASQLEATGKFKVINDGEFFNEFTVTSQESPAALNRRLQ
                     DANIIGGLDLGTIAPEMDGKLLLSATEMTGKAAIDRFVEVASAT"
     gene            1328..2665
                     /gene="der"
                     /locus_tag="VFV93_11545"
     CDS             1328..2665
                     /gene="der"
                     /locus_tag="VFV93_11545"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX5166022.1"
                     /translation="MKPIVAIVGRPNTGKSTIFNRLVGERRAIVEDLPGTTRDRLYGE
                     TDWIGVPFTIIDTGGLQEEDEFDRLTAREISVRTQNQARLAIEEADVILFLVDGRTGL
                     TAADHEVANIIRESGKPTVLGVNKAENEERRENAVEFWELGLGEPIAFSALHGIGTGD
                     VLDDVVRLLPRGDEESDEVEYPSFAVVGRPNVGKSSLLNALIGHERSIVSDVPGTTRD
                     SIDTLVNWAGNTVRLIDTAGIRRRGRIEPGVEKHSVLRAERAIDRADVALVVLDADEG
                     FTAQDQHIAGYVTEAEKGIVFIVNKWDLIAKDERTMNVYRDEAAEFFQFAPWAPMIFV
                     SALTGQRVNQIIDMALHVYGERQKRISTGELNRLLREAVAKHPPPSRPSSWLKLYYAT
                     QSDVAPPRFVFFCNDPKNVHFSYQRYLENEIREHYAFDGTPIVLHFRGRRRDD"
     gene            2658..3275
                     /gene="plsY"
                     /locus_tag="VFV93_11550"
     CDS             2658..3275
                     /gene="plsY"
                     /locus_tag="VFV93_11550"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX5166023.1"
                     /translation="MTDAFVLMLAYLIGSFPTGFVMTRLITGMDLRAVGSGGTGATNA
                     RRALGTKWGALVALIDVLKGLLAVGIARWLDAGDLTVTVAAILVVVGHCWPVWLGFRG
                     GKGVATGGGAAFALSPWGLFLIPFLVVPVAVTRYVSLGSLTAAVAAPVIFGILAIADR
                     TPGMYIIYAAVVAAVIIWRHRENIERLRRGTERKLSGGASTAGNG"
BASE COUNT          604 a         1113 c         1117 g          605 t
ORIGIN      
        1 ttcctcggcg ctggaagcta cacccactac gtaccggccg ccgtccagca gatcaccttc
       61 cggggcgagt tctacacggc ctacactccg taccagccgg aagttgctca gggcacgctc
      121 caggcgatct acgagtacca gtcaatgatc tgcgcgctaa cggggatgga agtctccaac
      181 gcgtcgatgt acgacggagc gacggcgctc gccgaaggag cgctgttgac ggtctcgctt
      241 ccgcgcaagc ggacgcggat cgtcatggcc gggacgattc atccgaacta cgccggtgtc
      301 gttcggacct acacgagcgg ggttggcgtc gaagtcgtcc agttgcccct gccggccgat
      361 gggctcaaga ccgacccggc cgcattcgat gaatacctcg atgacaacct ggcctgcatc
      421 gtcgtccagt atccgaactt cttcggcacg atcgaggaca tcgcggcgat cggcgagaag
      481 gcgcactcgt gtggggcgaa cctgatcgtc tcgacctacc cgaccgcgct cgggctgctg
      541 aaatcgcctg gtgagctcgg cgccgatgtt gtcaccggcg agggccagcc gctcggcaac
      601 gcccaatcgt tcggcggtcc ggtcgtcggc attctggcga cccatcagcg gctggtccgg
      661 caaatgccgg ggcggctggc aggcatcgcc tacgacagcg agggcaagcg cgggtttgtg
      721 ctggcgctgc aaactcgcga gcagcacatt cgccgcgaga aggcgaccag caacatctgc
      781 acgaatcagg gactcatggc gctggccgcg tctgtctacc tcgcgacgct cgggccgcag
      841 gggttgcgcc ggatcgccga gatctgctac cagaacggcc actacctcgc cagccagctt
      901 gaagcgaccg gcaagttcaa ggtcatcaac gacggtgagt tcttcaacga gttcaccgtc
      961 acgagccagg agtcacccgc cgcgctgaac cgccgcctcc aggacgcgaa catcatcggc
     1021 ggcctcgacc tcggcacgat cgcgccggag atggacggca agctgctgct gagcgcaacc
     1081 gagatgaccg gcaaggcggc catcgaccgt ttcgtcgagg tcgcctcggc gacataagtc
     1141 cacccgggtg gactccaaac ggcccgcgat tgctgtcgcg ggccgcttgc tgcctcctcg
     1201 ctcgaaaccg gatcacgcgt tcgcccctgg gcggtggacg acaggggcga acgcgcgggc
     1261 ggacgtcggg attgatacac tttcagttgg attactgcga cccgcaatca ggaaagcacc
     1321 aacgacattg aaaccgatcg tcgccatagt tggtcgcccc aacaccggca agtccaccat
     1381 ctttaaccgg ctcgtcggcg agcggcgcgc catcgtcgaa gacctgccgg gcacaacccg
     1441 tgaccgcctg tacggggaaa ccgactggat cggcgtcccg ttcacgatca tcgacactgg
     1501 cggcctccag gaggaggacg agttcgaccg gttgacggcg cgcgagatct cggtccggac
     1561 gcagaatcag gcgcggctcg cgatcgagga agcggacgtg atcctctttc tcgttgatgg
     1621 ccgcaccggc ctgaccgccg ccgaccacga ggttgccaac atcatccgcg agtcgggcaa
     1681 accgaccgtc ctgggtgtca acaaggccga gaacgaggaa cgtcgcgaga acgccgtcga
     1741 gttctgggaa ttgggcctcg gcgagccgat cgcgttctcg gccctgcatg gaatcgggac
     1801 cggcgatgtc ctcgacgatg tcgtccggtt gttaccgcgc ggcgacgagg aatccgatga
     1861 ggtcgagtac cccagcttcg ccgtcgtcgg gcgcccgaac gtcggcaagt cgtcattgct
     1921 gaacgcgctg atcggccacg agcgctcgat cgtctcggat gtgccaggga cgacacgcga
     1981 ctcgatcgat acgctcgtca actgggccgg caacacggtc cgtctgatcg acacggccgg
     2041 tatccgccgt cgcgggcgga tcgagccggg agtcgagaag cacagcgtcc tgcgcgccga
     2101 gcgggccatc gatcgcgccg atgtcgcgct ggtcgtcctc gatgccgacg agggtttcac
     2161 ggcccaggac cagcacatcg ccggctacgt caccgaggcc gaaaaaggga tcgtcttcat
     2221 cgtcaacaag tgggacctga tcgccaagga cgagcgaaca atgaacgtct accgcgacga
     2281 ggcggccgag ttcttccagt tcgcgccctg ggcgccgatg atcttcgttt cggccctgac
     2341 tggccagcgc gtcaatcaga tcatcgatat ggcgctgcat gtctacggcg agcggcagaa
     2401 gcgcatctcg acaggcgagc tcaaccggct gttgcgtgag gcagtcgcga agcatccgcc
     2461 gccctcgcga ccgagcagct ggctgaagct gtattacgcg acgcagtccg atgtcgctcc
     2521 gccgcgattc gtcttcttct gcaacgatcc gaagaacgtc cacttctcct accagcgcta
     2581 cctggagaac gagatccgcg agcactacgc attcgacggt acgccgatcg tgctgcactt
     2641 caggggccgt cgccgcgatg actgacgcgt tcgtcctcat gctggcctat ctgatcggct
     2701 cgttcccaac cgggttcgtg atgacccggc tgatcaccgg catggacctg cgggcggttg
     2761 gcagcggcgg caccggcgca accaacgcac ggcgggcact gggcacgaaa tggggcgcgc
     2821 tggtggccct gatcgacgtt cttaaagggt tgcttgccgt cggcatcgcg cgctggctcg
     2881 atgccggcga cctgacagtt acggtcgcgg cgattctggt cgtcgtcggc cattgctggc
     2941 cggtctggct gggttttcgg ggcggcaagg gcgtcgcgac cggtggcggc gcggcattcg
     3001 cgctctcacc ctggggactg tttctgatcc cgtttctggt cgtaccggtc gccgtgacgc
     3061 gctatgtctc gctggggtcg ctgacggccg ccgtagccgc accggtcatc ttcgggattc
     3121 tggcgatcgc ggatcggacg ccggggatgt acatcattta cgccgccgtt gtcgcggcag
     3181 tgatcatctg gcggcaccgc gagaacatcg agcgattacg gcgcggaacg gagcggaagc
     3241 tcagtggcgg ggcatcgacg gccggcaacg ggtagactaa ctgacagatg aacggcgcag
     3301 ccttcgcgct ctcaccttgg ggactgtttc tgatcccgtt tctggtcgta ccggtcgccg
     3361 tgacgcgcta tgtctcgctt gggtcgattt cagccgccgt ggccgcgccg attatcttcg
     3421 cgattctggc ggtcgcggg
//
LOCUS       DASUGJ010000514         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_80485, whole genome shotgun sequence.
ACCESSION   DASUGJ010000514 DASUGJ010000000
VERSION     DASUGJ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80485"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFV93_11555"
     CDS             <1..189
                     /locus_tag="VFV93_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166024.1"
                     /translation="ILGAPYGADMRHFIQIGGIPCLMYGAGDVALAHQADEHILLSEI
                     QTAAKTIAILLIDWCGAE"
     gene            495..728
                     /locus_tag="VFV93_11560"
     CDS             495..728
                     /locus_tag="VFV93_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166025.1"
                     /translation="MIDEELVWQRREITGETVRVCENCGRPMDIALLRQAADDTTDDG
                     GPGMRICPDCLDAMARGVDPVELDDEDDLVPVL"
     gene            821..1723
                     /locus_tag="VFV93_11565"
     CDS             821..1723
                     /locus_tag="VFV93_11565"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5166026.1"
                     /translation="MSIVERQYTDADFPRVLTLLGACRRAGYVDMELLSTNLRLTLTD
                     PAFDYRHTLLQERSGSLDAFALLWQGRYLAMLVAPNMRGRRETGVLVWAEHQMIAIGD
                     HARLAVLCRDDDALMVRFCETHGFALDDEELRMGRLLDDSLPIVAAPPGIVIRPLRGT
                     TELEDWLALYTEAFGPRIAQLRRWRAMRDDPDHEPSLDLIAAQRDGTLVGMCYCSIAS
                     FEASNDTVVDGRTEPIAIRHAFQRQGLGRALVSAGLTALRTAGAGVATLTTEVDNIAA
                     HRFYATLGYRELYRARWYGRDLQR"
     gene            2087..2488
                     /locus_tag="VFV93_11570"
     CDS             2087..2488
                     /locus_tag="VFV93_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5166027.1"
                     /translation="MSATGSRQLVSSGTVWEGIVGYSRAIRVDRFVYVSGTTATDDDG
                     NAVGANDPAAQTDYIIQKIERALSEAGASLRDVVRTRIYVTNADDWEAIGRVHGRYFA
                     DIRPSNVLVEVSRLVGSDYLVEIEADAVLDA"
     gene            2568..>3384
                     /locus_tag="VFV93_11575"
     CDS             2568..>3384
                     /locus_tag="VFV93_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5166028.1"
                     /translation="MRFSLRLNNDLPITEYVLLAQAAEEAEFDQFWVSHDLFLRSSPA
                     ILTAVALATERIELGTCILNPYTLHPAELAMVAATLEEVSEGRFNLGLGAGAAEFMKW
                     VRLPYDRPLATTRRAIRQLRAVLDGAQPPGWEPEAYLRFDSGSGRRIPIYLAALSPNM
                     LRLAGELADGVLPLLFPPEHYATVEPLIREGAERAGRSMAEIDLAACVWCSISDDRQA
                     AEDVLRDKIAYYGHALSPLIFDRLGLTADDFRATERALMTERDPLKARALVTDR"
BASE COUNT          570 a         1067 c         1116 g          631 t
ORIGIN      
        1 atcctcggcg cgccctacgg ggccgacatg cgccacttca tccagatcgg cggcataccc
       61 tgcctgatgt acggggctgg cgacgtcgcc ctcgcccacc aggccgacga gcacattctg
      121 ctctccgaga tccagacggc cgccaagacg atcgctatcc tgctgatcga ctggtgcggg
      181 gccgagtaat tcgggatacg cgacatggtg gttgaccgcg atccagtcga acggcgttcg
      241 gctgggtcac cgaatccaac tgcgccgcgc agcactcgcg gttgtcacac gattgtgacg
      301 gctggccgga agaacggggc gggatcgtca cgggcgctgc ggcatgatcg gagcggcggc
      361 ggcagccacg atgtgcgtat tatggaaata cgggcgaacg tggtatggtc gtcagttctg
      421 agcgggcatc gagggcaacg ggattgcggt tctcgatgtc gttgcgaagc tcgtgtacga
      481 agggatggtt cgcgatgatt gacgaagagc tggtctggca gcgacgcgaa atcaccgggg
      541 agacagtccg cgtctgcgag aattgcggcc ggccgatgga tatcgcgctc ctgcggcagg
      601 ctgctgacga cacgaccgac gacggcggcc ctggtatgcg catctgcccc gactgcctgg
      661 atgcgatggc gcggggtgtc gatccggtcg agctagatga cgaagacgat ctggttccgg
      721 tgctctaacg tcaccatcgg aatcgagaga cgatgccgcc acgcactgtg gcggcatcta
      781 tgtttccgca gcggcactca cgtcatgaca ggagcacagg atgtcgatcg tcgagcgcca
      841 atataccgat gccgactttc ctcgcgttct cacgttgctc ggcgcttgcc ggcgtgcggg
      901 ctatgtcgac atggaactgc tgtcgacgaa cctgcggctg accttgactg atccggcctt
      961 cgactatcgc cacacgcttc tacaggaacg gagcggtagc ctggatgcgt tcgcgctcct
     1021 ctggcaaggt cgctatctcg caatgctggt tgcgcccaac atgcgcggcc gacgtgagac
     1081 gggtgtgctc gtctgggccg agcaccagat gatcgcgatc ggcgaccacg cccgcctggc
     1141 cgttctctgc cgcgatgatg acgctctgat ggtccgattc tgcgagacgc acgggttcgc
     1201 acttgatgat gaggaactgc gaatgggcag gctgttggac gattcgctgc cgatcgtcgc
     1261 ggcaccgccg ggcatcgtga ttcggccgct acggggcacg accgaactgg aggactggct
     1321 tgcgctgtac accgaagcgt tcggtccacg aatcgcgcag ctccggcgct ggcgcgcgat
     1381 gcgcgacgat cccgatcatg aaccgtcgct cgatctcatt gcggcgcagc gtgacggaac
     1441 gttggttggg atgtgctact gctcgatcgc gtcgttcgaa gcgtcgaacg acacggtggt
     1501 ggatggacga accgagccga tcgccatcag gcatgcgttc cagcgacagg gactcggtcg
     1561 agcgctcgtc agcgccggac tcaccgccct gcgaactgcg ggagcgggag tcgcgaccct
     1621 gacaacggag gtcgacaata tcgctgcgca tcggttctac gcaacgctcg gctacaggga
     1681 gctctaccgg gctcgctggt acggacgcga tctgcaacgc tgatagcggt cgcctagcta
     1741 gattgctgtt gctgctgggt cacgctcttg ccaggaacgc gctcgccggg tgttgccggt
     1801 ttggctgccg gtttcgcctt cttgggtttc cgcgtctcac ggtttgaacg gttcttgtct
     1861 ttcgccacgg gatcctccgt ccgacaaata tcgcgacaag catccatccg ccgtacctca
     1921 catcatagtc ctcgaagagt gagaagggaa tagcgctgcg accatcgaga tgtggtgtcc
     1981 ctgccggacc aacgttcgcc cgcgtcgagt gtgtaccggc tgccggctcc gagttggggt
     2041 accatgcctc cacaatacgc cgtatcgatc gaatgaggga gccgtcgtga gcgcaactgg
     2101 atcgcggcaa ctggtgtcgt cggggaccgt ctgggagggg atcgttggtt actcgcgggc
     2161 gatccgcgtt gaccgtttcg tctacgtgtc cggcacgacc gcgaccgatg acgacggcaa
     2221 cgccgtcggc gccaacgatc cagccgcgca gaccgactac atcatccaga agatcgagcg
     2281 ggcattgagc gaagccggcg ccagcctgcg cgatgtcgtc cgcacccgca tctacgtcac
     2341 caacgccgac gactgggagg cgatcggtcg cgtccacggc cgctacttcg ccgacattcg
     2401 cccctccaac gtcctcgtcg aagtcagccg tctggtcggc agcgactatc tggtcgagat
     2461 tgaggcggat gcggtgctgg acgcttgatg ggggatggga atgtgcgtcc tcgcatccct
     2521 catcctccat cccccatccc tcactacgta ccgaaggaca acgccgcatg cgcttctcgc
     2581 tgcgtctcaa caacgatctt cctataacgg agtatgtcct gctggcgcag gcggccgagg
     2641 aggctgagtt cgaccagttc tgggtcagcc acgacctgtt cctgcgctcg tcgccggcga
     2701 tcctgacggc agtggcgctg gcgaccgagc ggatcgagct tggcacctgc atcctgaacc
     2761 cgtacacgct ccaccctgcc gaactggcga tggtggcggc gacgctggag gaggtcagcg
     2821 aggggcggtt caacctcggg ctgggggccg gagcggccga gttcatgaag tgggtccggc
     2881 tgccctacga ccggccactg gcgacgacgc gacgggccat ccggcagttg cgggctgtcc
     2941 tggatggcgc gcagcctccc ggttgggaac cggaagcgta cctgcgcttc gattccggtt
     3001 caggccgccg gattccgatc tacctggcgg cgctctcgcc gaacatgctg cggctggccg
     3061 gcgagctggc cgatggcgtc ctgccgctgc tgtttccgcc cgagcattac gcgaccgtcg
     3121 agccgctgat ccgcgagggg gccgagcggg ccgggcgcag catggcggag atcgacctgg
     3181 ccgcctgtgt ctggtgctcg atctcggacg atcgccaggc ggctgaagat gtcctgcgtg
     3241 acaagatcgc ctactacggc catgcccttt cgccgctcat cttcgatcgg ctcggcctga
     3301 ccgccgacga cttccgcgcg accgaacggg cgctgatgac cgagcgcgac ccgctcaagg
     3361 cccgcgcgct cgttaccgac cgga
//
LOCUS       DASUGJ010000515         8873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1610910, whole genome shotgun sequence.
ACCESSION   DASUGJ010000515 DASUGJ010000000
VERSION     DASUGJ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8873
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610910"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3266)
                     /locus_tag="VFV93_11580"
     CDS             complement(<1..3266)
                     /locus_tag="VFV93_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HEX5166029.1"
                     /translation="MAAEPVRAEGAGADAVLPRLESARRELLDLGLRNSLINYRPLKS
                     RGVEAVGESPVEVFRSLVREGKRMSFAAAPETKTTAVQTTLPETEPLPALAQPDEPSP
                     EEVSARQRDSQLQTAVTSEQLQTRLLNTFYAARTFVEEQGVNVLYLALGMLEWYETTT
                     SEEKRRAPLILVPVELERSNALDRFHLTYLGDEIGDNLSLAAKLKAEFGLEAPIMPEV
                     EDLDIDAWFDQWATAVASRPRWAVDRSAVALGFFSFGKFLMYHDLNTATWPDGAGPTQ
                     HPIVGALLAESGFSEPPSAIADDAFLDPLIKPGEVRQVVDADSSQTLAILDVNAGRNL
                     VIQGPPGTGKSQTITNLIAEAIGAGKTILFVAEKMAALEVVKRRLDTVGLGEACLELH
                     SNKINKRSVLAELRRTLELGRPKVDELRSELSLLEASRARLNAYCDAVNAPVGTSGVT
                     PYIAYGELIRSRATPGSGEWPKLELPAMAGWSEVEYVQRRAILQELQARLAVMGTPVE
                     HPFWGSNRTVLLPSEADALRNELEAAPADLAALRDATAALADLLELSHATNRLETETL
                     LNAATTALSAPDLRGINIAAPAWASQVPAIRELIDAGTAETAIRSKRSADVIPEAWDA
                     DLLPVRQSLAANRDKFFRFLSGEYRGAKSRLAGYCTAGLPDGVDAQIALIDDILEARR
                     QAEVVSRHAGLGQTLFGGSLQDGATDWARISAVAEWAIGFHAGVAAEAIPAGLVSYLA
                     GSPDPAPVGQARAAVVEAQTHWDTRLNALHSLLQFDDQRRYGQDALLADQPYTVLDEL
                     LRVWTLRRNDIDQVLAFNQSAAACHEAGLDDVIAVAERWPAGGQSLVAAFQRGWYSVL
                     LEAALRERPELATFDSTSHEQVIARFRELDQLVFAYNRSRLALAHWQRLPQHEAGGQL
                     GVLRREFEKKSRHLPIRQLMTRAGNAVQAIKPIFMMSPLSIATFIPPGVLSFDLVVFD
                     EASQIKPVDAFGALARGKQAVVVGDDKQLPPTSFFDTMASADEIDETTVSDIPSILGL
                     FNSQSAPKRMLRWHYRSRHESLIAVSNHEFYENKLVVFPSPDHSRAEAGLVFRHLPET
                     A"
     regulatory      3593..3701
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3853..4683
                     /gene="thiD"
                     /locus_tag="VFV93_11585"
     CDS             3853..4683
                     /gene="thiD"
                     /locus_tag="VFV93_11585"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX5166030.1"
                     /translation="MSLSYAVPAALTIAGSDSGGGAGIQADLKTFAALGVYGTSAITA
                     VTAQNTLGVDSFVALAPEMVAAQMRSVIADIGPRAAKTGMLANADIVRAVADVLTEHG
                     DLPLVIDPVTHAKSGDALLADDAVGVLRELLIPRCAVVTPNLPEAAALVGFPVECEAE
                     MLEAGLALLDAGAIAVVVKGGHRPDTADDLYLDRDSVEWLRSERIATRCTHGTGCTFS
                     AAIAAGLARGMTTFDAVRAAKGYLTAAMRAGYEVGAGHSPVNHFYAFDAVTQHEGIVS
                     "
     gene            4680..5486
                     /locus_tag="VFV93_11590"
     CDS             4680..5486
                     /locus_tag="VFV93_11590"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX5166031.1"
                     /translation="MTATVTPTDTIVDKPLVIGGREFSSRLLLGTGKYRTFDEMVSAF
                     EASGTQIITVALRRIDFDDPESRSVLEKVDWSNYQILPNTAGCKTADEAVRIARLARS
                     MGLSDWIKLEVIPDPLYLFPDAVGTLDAARTLVAEGFTVLPYINDDPVLAKQLEEIGC
                     ATVMPLAAPIGSGQGLLRTDRIQMIVEQSSVPVVVDAGIGVPSDASLAMETGADAVLV
                     NSAIALARNPAQMARAMALGVEAGRTAWLAGRMEKRQTASPSSPTTGISK"
     gene            5483..6112
                     /gene="thiE"
                     /locus_tag="VFV93_11595"
     CDS             5483..6112
                     /gene="thiE"
                     /locus_tag="VFV93_11595"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX5166032.1"
                     /translation="MTDLASLLRCYLVTDARAGSVERLVEISQAALAGGVTTIQLRAK
                     GWSDRQLLDAAMALRPICRDAGALFIVNDRVDIVLAGGADGVHLGVDDLPVAVARQLL
                     GPDAVIGYSPESDADRVRAEADGADYLGVGPVYGTGTKLDAGDAIGLGGLERVVSATT
                     LPVIGIGGITIERSAEVVSTGAVGVAVVGAVFLAGDPSAAARRLREVLQ"
     gene            6109..6726
                     /locus_tag="VFV93_11600"
     CDS             6109..6726
                     /locus_tag="VFV93_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX5166033.1"
                     /translation="MTVRPVPRLHVIGPLITDPADYPAIAVAAVKGGCDAVHVRIPGG
                     ATDDLLALARTLRDLIGDVALIVNDRLDIALLAGAAGVQLGERSFSVADARQLLPAGA
                     LIGRSVHDLDGARAALADGADYLLAGHVFDTPSKQGVPGRGLDWLAELASVVPIPVIA
                     LGGITIERVPGVLEAGAHGVALGRELLRSTDPAASARRVAEILFQ"
     gene            6748..6972
                     /gene="thiS"
                     /locus_tag="VFV93_11605"
     CDS             6748..6972
                     /gene="thiS"
                     /locus_tag="VFV93_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX5166034.1"
                     /translation="MAQTSMATITVQLNGIDVTVPHGMTLGGLIDNRGIERRMIAVEY
                     NAEILPRHAYDATILNDGDRLEVVQMVGGG"
     gene            6974..7645
                     /gene="tenA"
                     /locus_tag="VFV93_11610"
     CDS             6974..7645
                     /gene="tenA"
                     /locus_tag="VFV93_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEX5166035.1"
                     /translation="MNDGSFFATLAAENAATHAAGLEHPAVLGIGDGSLAETTFRYYI
                     EQDYNYLRRYIRVLALAVAASPDLPTATRLAELVSATLSVEVGALVELYAAFGGNRAE
                     LDAIRQSPTCQAYTDHLLATATGSNLFVIMASVLPCQWGYREIGHHLLRRGLPADTRY
                     ARWIKEYASDEYGDLVDWLICRFDELAATESATTRQSAREAFALSTHYEHAFWEMAWV
                     RERWA"
     gene            complement(7696..>8873)
                     /locus_tag="VFV93_11615"
     CDS             complement(7696..>8873)
                     /locus_tag="VFV93_11615"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX5166036.1"
                     /translation="WDEDYADARERLKNIWDAPVEVVAAGEVWTWTAGDISNWIEIHQ
                     AGGPGKAASISINQQWIDDVVNLIATDTNHAPQSARVWWDVSGNLIKTADGNPGKRLQ
                     WDESRALIQATFLGENTTNRLDLPVEISNPPALPADLNTLGINSFLGEASTGYGGGLP
                     ERMHNIELAASRLNGTIVMPGQTFSFNGEVGPTTLDAGFQIAYGIANEGGQLTTIPSE
                     AGGICQVATTVFQPVFWQGYQIDERSTHSYWIPNYASNGYVGLDATVDEASGLDFKWT
                     NNTATAVLIEAVADGENFTVRLYGTAPPWSVQVDEPKTSNVKEADEEIVYQASDTIPD
                     GETRRIESAHDGFDVTVTRHVTQNGETQTENFSTSYGPSRNVVLVGSSTGELPAEYR"
BASE COUNT         1406 a         2969 c         2951 g         1547 t
ORIGIN      
        1 gcggtctccg gcaggtggcg gaagaccaga ccggcctcag cgcgcgagtg gtccgggctg
       61 gggaagacga ccagcttgtt ctcatagaac tcgtggttgg agacagcgat cagcgactcg
      121 tgccggctgc ggtagtgcca gcgcagcatc cgcttcggcg cgctctgcga gttgaacagc
      181 ccgaggatgc tgggaatgtc gctgacggtc gtctcgtcga tctcgtcggc gctcgccatc
      241 gtgtcaaaga agctggtcgg cggcagttgc ttgtcgtcac cgacgacgac ggcctgcttg
      301 ccccgtgcca gcgcgccgaa ggcatcgacc ggcttgatct ggctggcctc gtcgaagacg
      361 accaggtcga agctcagcac gccgggcggg atgaaggtcg cgatcgagag cgggctcatc
      421 atgaagatcg gcttgatcgc ctggacagcg ttgccggccc gcgtcatcaa ctggcggatc
      481 ggcaggtggc gcgatttctt ctcgaactcg cgccgcagga cgccaagctg gccgccggct
      541 tcgtgctgcg gcagccgctg ccagtgcgcc agcgccagtc gggagcggtt gtacgcgaag
      601 acgagctggt ccagttcgcg aaagcgcgcg atgacctgct cgtgggaggt cgagtcgaac
      661 gtcgccaatt ccggccgctc gcgcaaagcg gcctccagca ggacgctgta ccaaccgcgc
      721 tggaacgccg cgacgagaga ctggccaccc gccggccagc gctcggcgac ggcgatcacg
      781 tcatccagcc ccgcttcatg gcaggctgcc gccgactggt tgaaggccag cacctggtcg
      841 atgtcgttgc gccgcagcgt ccagacacgc aggagctcgt ccaggactgt gtacggctga
      901 tccgcgagca gcgcgtcctg cccgtaccgc cgctggtcat cgaactgtag cagcgagtgg
      961 agcgcgttga gccgggtgtc ccagtgcgtt tgtgcctcga cgacggcagc gcgggcctgc
     1021 ccgaccgggg ctggatcggg cgatcccgcg agatagctca ccagccctgc cggaatcgct
     1081 tcggcggcaa cgccggcatg gaacccgatc gcccactccg cgacggcgct gatgcgtgcc
     1141 caatcagttg cgccgtcctg caagctgccg ccgaacagcg tctggccgag tcccgcgtga
     1201 cgactgacga cctctgcctg gcgccgtgcc tccagaatat cgtcgatcag ggcgatctgg
     1261 gcgtcgacgc cgtccggcag cccggctgtg caatagccgg ccagcctcga cttggccccg
     1321 cgatactccc cggagaggaa gcggaagaac ttgtcgcgat tggcggccag gctctggcga
     1381 accggcagca gatcggcatc ccaggcctcg gggatcacat ctgccgatcg cttgctgcga
     1441 atcgccgtct cggccgttcc ggcatcgatc agttcgcgga tcgcgggaac ctggctggcc
     1501 cacgcgggag cggcgatgtt gattccgcgc aggtccggtg cgctcaatgc cgtcgtcgct
     1561 gcgttcagga gagtctcggt ttccagtctg ttcgttgcgt gggaaagttc gagcagatcg
     1621 gccagcgcag ccgtggcatc acggagggct gcgaggtcag caggcgcggc ctccaactcg
     1681 ttgcggagtg cgtcagcctc ggatggcagg aggaccgtcc ggttgctgcc ccagaaggga
     1741 tgctcaaccg gggtgcccat cacggccagc cgcgcctgga gttcctggag gatcgctcgc
     1801 cgctgaacgt actcgacctc ggaccagccc gccatcgccg gcagctccag cttcggccac
     1861 tcgccgctgc ccggggtcgc ccgcgagcgg atcaactccc cataagcgat gtacggagtc
     1921 acgccgctcg tgccgaccgg cgcgttgacg gcatcacagt aggcgttgag ccgggcgcga
     1981 ctcgcttcca gcagcgatag ctccgagcgc agctcgtcga ccttcggcct cccgagctcc
     2041 agtgttctcc gcagttcggc caggacggag cgcttgttga tcttgttgga gtgcagctcc
     2101 aggcaggctt cgccaagccc gaccgtgtcg agtcgccgct tgacgacctc cagcgctgcc
     2161 atcttctcgg cgacgaacag gatcgtcttg ccggccccga tcgcttcggc gatcaggtta
     2221 gtgatcgtct gcgacttgcc ggtgccgggc ggcccctgaa tgacgaggtt acggccggcg
     2281 ttgacatcca gaatcgccag cgtttgcgag gagtcggcat cgactacctg gcggacctca
     2341 cccggcttga tcagcgggtc caggaaggca tcatcggcga tggcggatgg cggctcggag
     2401 aagccggact ccgccaacag agcgccgacg atcgggtgct gggttgggcc ggctccgtcc
     2461 ggccaggtcg cggtgttgag gtcgtggtac atcaggaact tgccgaacga gaagaagccc
     2521 aacgcgacgg ccgagcggtc gaccgcccag cgcggccggc tggcgacggc ggtggcccac
     2581 tgatcgaacc aggcgtcgat gtcgaggtcc tctacctcgg gcatgatcgg cgcctcaagc
     2641 ccgaactccg ccttcagctt ggccgccagc gacaggttgt cgccgatctc gtcgccgaga
     2701 taggtcaggt ggaagcggtc gagcgcgttg ctgcgttcca gctcgacagg aacgaggatc
     2761 aacggcgccc ggcgcttctc ctcgctggtc gtcgtctcat accattcgag catccctagc
     2821 gcgaggtaga gaacgttgac gccctgctct tcgacgaacg tgcgggcggc atagaaggtg
     2881 ttcagcaacc gcgtctgcaa ctgctcgctg gtgacggccg tctgcagctg tgaatcgcgt
     2941 tggcgggcgg agacctcctc cggcgatggc tcatcgggct gggccagcgc cggtagcggt
     3001 tcggtttctg ggagcgtcgt ctggacggcg gtcgtcttcg tctccggcgc cgccgcgaac
     3061 gacatgcgct tgccctcgcg gacgagactc cggaacacct cgaccggcga ctcgccgact
     3121 gcttcgacgc cacgcgactt gagcggccgg tagttgatca gcgagttgcg caggccaagg
     3181 tccagcagct cgcgccgcgc gctctccagc ctgggcagga cagcatctgc cccagccccc
     3241 tccgcccgca caggttcagc ggccatcgat ccggttcctt cccgcgctac cacagcgctc
     3301 cattgccgac gatgcttgca taataacagt tcctgtgcgc cgaatctgaa acggtatcgc
     3361 atccgtgtag gggccgatcc tgtatcggcc cgcgccgtga ggcgcccggt gaattgaatg
     3421 gtcggttacc ccacaggtgc aggcgttagg gtgcctcggc aaaacgccat ggatctagaa
     3481 ggcgcatcac ggcgcgagcc gatacgggat cggcccctac ccggatatag gttcggcgcg
     3541 cacatcaaac ccaaccgcgc atgaaagcct gctgtgctag actagatgca gcgacggatg
     3601 ccaggggtgc gcgtaaagcg ctgagattgc ggggaaggcc cgctgaccct ccgaacctga
     3661 tccgggtaat tccggcgtag gaacggcatc gaaaaccagt gtcaccactg cgccgtcggc
     3721 cctcgggccg cggcgtgttt gctttccagc cactggtttc cgacgacagg gcctccccaa
     3781 tctccccggc aatccagcgc cgcccgctca tcgacgacag cgggcgtggt tgcgcgatga
     3841 agggaggaag ggatgtcgct ctcttacgcg gtcccggctg cgctgacgat cgctggatct
     3901 gactccggcg gaggcgctgg cattcaggcc gatctcaaga cattcgccgc gctcggggtc
     3961 tatggaacgt cggccatcac ggctgtgacg gcccagaaca cgctcggcgt cgatagcttc
     4021 gtcgccctcg cgccggagat ggtcgctgcc cagatgcgct cggtcatcgc cgatatcgga
     4081 ccgcgcgccg ccaagaccgg catgctggcg aacgccgata tcgtccgagc cgtcgccgac
     4141 gtgttgacgg aacatggcga tctaccgctc gtgattgacc cggtcacaca cgctaagagc
     4201 ggcgatgcgc tgctggctga tgatgccgtc ggcgtccttc gcgaactgct gatcccccgc
     4261 tgcgcggtcg ttacacccaa cctgccggaa gcggctgccc tggtcggctt cccggtcgag
     4321 tgcgaggcgg agatgctcga ggccgggctg gctttgctgg atgccggcgc aatcgccgtc
     4381 gtcgtcaaag gcgggcatcg gcctgacacg gccgacgatc tctacctcga ccgcgactcg
     4441 gtcgagtggt tgcgttcgga gcggatcgcg acccgctgca cgcacggcac cggttgcacc
     4501 ttctcggcgg cgatcgccgc cggccttgcc cgtgggatga caacgttcga cgccgtccgc
     4561 gccgccaagg ggtacctgac ggctgcgatg cgggcaggct acgaggtcgg cgccggtcac
     4621 tcgcccgtca accatttcta cgcgttcgat gccgttacgc aacacgaagg gatcgtctca
     4681 tgaccgcgac tgtcacgccg accgacacaa tcgtcgacaa gccgctggtg atcggcgggc
     4741 gcgagttcag ctcgcggctg ctgctcggaa ccggcaaata ccgcaccttc gatgagatgg
     4801 tcagtgcctt cgaggcgtcc ggtacccaga tcatcaccgt tgccctgcgc cggattgact
     4861 tcgacgatcc cgagagccgc agtgtcctgg agaaggtcga ctggtccaac taccagatcc
     4921 tgccgaacac ggccggttgc aagaccgccg acgaggccgt tcggatcgcc cgtttggccc
     4981 ggtcgatggg cctctcggac tggatcaagc tcgaagtcat tcccgatccg ctctacctct
     5041 tcccggacgc ggtcgggacg cttgacgcag cccggacgct ggtcgcagaa ggcttcacgg
     5101 tcctgcccta catcaatgac gacccggtcc ttgcgaagca gctggaggag atcggctgcg
     5161 cgacggtcat gccgctggcg gcgccgatcg gttccggcca gggactgcta cggaccgatc
     5221 gaatccagat gatcgttgag cagtcgagcg taccggtcgt cgtcgatgct ggaatcggcg
     5281 tcccaagtga cgcgtcgctg gcgatggaga ccggcgctga cgccgtcctc gtcaactcgg
     5341 cgattgcgct ggcgcgtaat ccggcccaga tggcgcgggc gatggcgctc ggcgtcgaag
     5401 ccggccggac tgcctggctg gccggtcgga tggagaagcg ccagaccgcc tcgcccagca
     5461 gcccgacgac gggaatcagc aagtgaccga ccttgcatcg ctcctgcgct gctatctcgt
     5521 gaccgacgcg cgggccggtt cggttgagcg gcttgttgag atcagccagg ccgcgctggc
     5581 cgggggcgtc accaccattc agctccgggc gaagggctgg agcgaccggc agttgctcga
     5641 cgccgcaatg gcgctgcgtc cgatctgccg agatgccggc gcgctcttca tcgtcaacga
     5701 ccgcgtcgat atcgtgctgg cgggcggcgc cgatggcgtc cacctcggcg tcgatgacct
     5761 gccggtcgcc gttgcccgcc agcttctcgg cccagacgcc gtcatcggat actcgccgga
     5821 atccgatgcc gatcgtgtcc gggccgaggc ggacggtgcc gattatctcg gcgtcggacc
     5881 cgtttacggc accgggacga agctcgacgc aggtgacgcg atcgggctcg gcggtctgga
     5941 gcgagttgtc tccgcgacga cgctgccggt gatcggcatc ggcggtatta cgatcgagcg
     6001 ctctgccgag gtcgttagca ccggcgcggt cggtgtcgcc gtcgtcggcg ctgtcttctt
     6061 ggccggcgat ccgtctgccg ccgcccgccg tctgcgcgag gtgttgcaat gacggtccgg
     6121 ccggtgccgc gtctgcacgt gatcggcccg ctcatcaccg atccggccga ctaccccgcg
     6181 atcgcggttg cggccgtcaa gggcggctgc gacgcggtcc atgtccgtat ccccggtggc
     6241 gcgacggatg atctgctggc gctcgcccgc acgctacgcg acctcattgg cgacgtggcg
     6301 ttgatcgtca acgaccggct cgacatcgcg ttgctggctg gcgccgctgg cgtccagctt
     6361 ggcgagcgga gcttcagcgt cgcggacgca cggcagcttc tgccggccgg ggcgctgatc
     6421 ggccgctcgg ttcacgacct cgacggcgcg cgggccgcgc tggcggacgg cgccgactac
     6481 ctgctggccg gccatgtctt cgacacgccg agcaagcagg gtgtccccgg ccgcggcctc
     6541 gactggctgg ctgagctggc ttctgtcgtt ccgatcccgg tcatcgccct cggcggcatc
     6601 acgatcgagc gggttcccgg cgtcctggag gcgggcgcac acggcgtcgc gcttggccgt
     6661 gagctgctgc gctcgaccga tccggccgcc agcgcccggc gcgtagccga aatcctgttt
     6721 cagtaacgac acaaggagga aagaacgatg gcacagacca gtatggcaac catcaccgtc
     6781 cagctcaacg gcatcgatgt gaccgtcccg cacgggatga cgctcggcgg gctgatcgac
     6841 aaccggggca tcgagcggcg gatgatcgcg gtcgagtaca acgccgagat cctgcctcgc
     6901 cacgcctatg acgcgacgat cctgaacgat ggcgaccggc tggaggtcgt tcagatggtc
     6961 ggaggtggct gacgtgaacg acggttcgtt ctttgcaacg ctggcggccg agaacgccgc
     7021 aactcacgcg gccggcctgg agcacccggc cgtcctcggc atcggcgacg gctcgctagc
     7081 ggagacgacg ttccgctact acatcgagca ggactacaac tacctgcgcc gctacatccg
     7141 cgtcctggcg ctggccgtcg ccgccagccc cgacctgccg accgccacgc ggctggctga
     7201 gctggtcagc gcaacgctgt cggtggaggt tggcgcgctg gtcgagctct acgcagcctt
     7261 cggcggcaac cgtgccgaac ttgacgcgat ccgccagtcg ccgacctgtc aggcgtatac
     7321 cgaccacctg ctggcgacgg caaccggcag caacctgttc gtcatcatgg ccagcgtcct
     7381 gccctgccag tggggctacc gcgagatcgg ccatcatctg ctccgtcgcg ggctaccggc
     7441 agacacccgc tacgcccgct ggatcaagga gtacgcctcc gacgagtacg gcgacctcgt
     7501 cgactggctt atctgccgtt tcgacgagct ggcggcaacc gaatccgcca ccacccgcca
     7561 atctgcccgc gaggcgttcg cgctcagcac ccactacgaa cacgccttct gggagatggc
     7621 ctgggtgagg gaacgctggg cataacgttg ctgcttcggg cgtgtcaaac acccaatcaa
     7681 aaccccaatc cggtgctagc gatattcggc cggtagttcg ccggttgacg agccgaccag
     7741 gacgacgttg cgggaggggc cgtaggatgt gctgaagttc tcggtctgcg tctcgccgtt
     7801 ctgggtcacg tggcgggtga cggtcacgtc gaagccgtcg tgggcgctct cgatccggcg
     7861 cgtctcgccg tcggggatcg tgtcggacgc ctggtagacg atctcttcgt ccgcttcctt
     7921 gacgttggag gtcttcggct catcgacctg gaccgaccac ggcggcgcgg tgccgtagag
     7981 ccggaccgtg aagttctcac cgtccgccac agcctcgatc aggacggccg tcgccgtgtt
     8041 gttggtccac ttgaagtcga gaccactggc ctcatcgacg gtcgcatcca gcccgacgta
     8101 gccgttcgag gcgtagttcg gaatccagta ggagtgggtt gaccgctcgt cgatctggta
     8161 accctgccag aagaccggct ggaagacggt cgtcgccacc tgacagatgc cgcccgcctc
     8221 ggacgggatc gtcgtcagct ggccgccctc gttggcgatg ccgtaggcga tctggaagcc
     8281 ggcatccagc gtcgtcggcc cgacttcgcc gttgaacgag aacgtctggc ccggcatgac
     8341 gatcgtgcca ttgagccgtg acgccgccaa ctcgatattg tgcatccgct ccggcagccc
     8401 gccgccgtag cccgtgctgg cctcgccgag aaacgagttg ataccgagcg tgttcagatc
     8461 ggcgggcagg gcaggcgggt tgctgatctc gaccggcaga tcgagccggt tggtcgtgtt
     8521 ctcgccgagg aacgtcgcct gaatcagcgc ccgcgactcg tcccattgca gccgcttgcc
     8581 ggggttgccg tcggcggtct tgatcagatt cccactgaca tcccaccaga cccgcgcact
     8641 ctgtggcgcg tggttcgtgt cggtcgcgat cagattgacg acatcgtcga tccattgctg
     8701 gttgatgctg atgctggcag ctttgcccgg tccgcccgcc tgatggatct cgatccagtt
     8761 ggagatgtca cccgcagtcc aggtccagac ctcaccggcc gcgacaacct cgaccggcgc
     8821 gtcccagatg ttcttgagcc gctcgcgggc gtcggcataa tcctcgtccc agg
//
LOCUS       DASUGJ010000516         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1470286, whole genome shotgun sequence.
ACCESSION   DASUGJ010000516 DASUGJ010000000
VERSION     DASUGJ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470286"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VFV93_11620"
     CDS             <1..1243
                     /locus_tag="VFV93_11620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5166037.1"
                     /translation="IDTGVTGLVARTGQAVFIKDVSASPDYLPTSPVIQQEICVPIRS
                     HGQVIGVLNVETPSSTPLEPSDFEILTTIANHLGMAFERSEAYEAERRSRTAMEAIQR
                     VATIVSSTLDSDEALRRIVEMLATVFGYSYVSISLIDGEQLDSTAWHGLPEGSVPPPF
                     ILGAGVAGRVAQTGLAELVDLFTADEESSIARADTTSQIVVPIRCSGELAGVISVEGT
                     EQQPLTRHDLELLQTFAEHAGTVISNARRYEQVQQLALRDPITDLPNYRQFQTRLNAE
                     LARSERHGRPLALLVIDLDGFKSINDAFGHLAGDEVLRQIGSRLLAELRESDVLARYA
                     GDEFVVILPETDRSLAQQIAGRLHSAIASQPFRVSSGQQVIVSLSIGVAAFPDDATSD
                     DGLVRAADSAMYQLKRGGPTA"
     gene            1319..1705
                     /gene="queF"
                     /locus_tag="VFV93_11625"
     CDS             1319..1705
                     /gene="queF"
                     /locus_tag="VFV93_11625"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX5166038.1"
                     /translation="MSTLLGANPTTATKDLEIFQNPNPERDYTIEISSPEFTCLCPVT
                     GQPDFAEITLRYVPDQYCVELKSFKLYLWSFRNEGAFHEAVTNQILDEVVGKIAPREA
                     TVIGKFNVRGGLYTTVTATHTAPGRE"
     gene            1718..>2230
                     /locus_tag="VFV93_11630"
     CDS             1718..>2230
                     /locus_tag="VFV93_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166039.1"
                     /translation="MAGLPPLVAEIESGVAIDHARLAELSDSERTAAVVALLQTPAGR
                     RWLRLDPELDADAADRLLIDDDFRQPRRDRSLVQVVERASLPEIERHVEAIAAGPAAS
                     TLWQRLAVDHERLVALATRLVADGGAQAAEATLHLLVLDQLDPYGIGVVARQQIASSA
                     LGSSAPVVGGR"
BASE COUNT          382 a          752 c          708 g          388 t
ORIGIN      
        1 gatcgacacg ggcgtgaccg gtctcgtcgc ccggactggt caggcggtct tcatcaaaga
       61 tgtcagcgcc tcgcccgatt atttgccgac cagcccggtc atccagcagg agatctgtgt
      121 cccgatccgc tcgcatggcc aggtgattgg ggtgctgaat gtcgagacgc cgtccagcac
      181 gccgctggag ccgagcgact tcgagatcct gacgacgatc gccaaccacc tcgggatggc
      241 gttcgagcgc agtgaggcct acgaggcgga acgacgcagc cggacggcga tggaggccat
      301 ccagcgcgtc gcgacgatcg tctccagcac actggattct gacgaggcac tgcgccggat
      361 cgttgagatg ctggcgacgg tcttcggtta ttcgtatgtg tcgatcagcc tgatcgatgg
      421 cgagcagctc gactcgaccg cctggcatgg cctgccggag ggcagcgtac cgcctccgtt
      481 catcctcggc gcgggtgtgg ccggtcgcgt cgctcagacg ggcctcgccg agctggtgga
      541 tctgttcacc gccgacgaag agtccagcat cgcccgcgca gacacgacct cgcagatcgt
      601 cgtcccgatt cgctgttccg gtgagctggc cggcgtcata agcgtcgaag gcaccgagca
      661 gcaaccgctc acacggcacg acctcgaact gctgcagacc ttcgccgagc acgcggggac
      721 ggtcatcagc aacgcgcgcc gctacgagca ggtccagcaa ctggcgctac gcgacccgat
      781 cactgacctg cccaactacc ggcagtttca gacgcggttg aacgcagagc tggctcgttc
      841 cgagcgtcac gggcgtccat tggcgctact cgtgatcgac ctcgatggct tcaagagcat
      901 caacgacgcg tttggccacc tggccggcga cgaagtgtta cgccagatcg gctcgcggct
      961 gctggctgag ttgcgcgaga gcgatgtcct cgcgcgctac gccggcgacg agttcgtcgt
     1021 catcctgccg gaaaccgacc gcagcctggc ccagcagatt gccgggcgcc tccactccgc
     1081 tatcgccagc cagcccttcc gtgtctccag cgggcagcag gtgatcgtct cgctctcgat
     1141 cggcgtggcc gccttccccg acgacgcgac gtccgacgac ggcctcgttc gcgccgccga
     1201 cagtgcgatg taccagctca agcgtggcgg accaacggcc tgaccttcgc cggcgtcgaa
     1261 cgggtacaat tgtgtgttgc gctgttgtgc tgcgttctac ctggagaaac gaggaaccgt
     1321 gtccaccctg cttggtgcca atccgacgac ggcgacgaag gacctggaga tcttccagaa
     1381 cccgaatccc gagcgggact acaccatcga aatctcgtcg ccggagttca cctgcctgtg
     1441 ccccgtcacc ggccagcccg atttcgccga gatcacgctg cgctatgttc ccgaccagta
     1501 ctgcgtcgag ctgaagtcgt tcaagctcta cctctggtca ttccgcaacg agggtgcctt
     1561 ccacgaagcc gtcaccaacc agattctcga cgaggtcgtt ggcaagatcg caccgcgtga
     1621 ggcgacggtc attggcaagt tcaacgtccg tggcgggctc tacacaaccg tcaccgcgac
     1681 tcacactgcg ccggggcgtg agtaagcagg ccggatcgtg gcaggacttc cgccactcgt
     1741 tgccgagatt gaatccggcg tagccatcga ccacgcccgg ctggcggaac tgagcgacag
     1801 cgagcggact gcagccgtcg tggcgctgct gcagacgcca gccggtcgtc gctggctgcg
     1861 attggacccg gaactcgacg ccgacgctgc tgaccgcctg ctgattgacg acgacttccg
     1921 ccagccgcgc cgcgacaggt cgctggtcca ggtcgtcgag cgggcatcgc taccggagat
     1981 cgagcgtcac gtcgaggcga tcgcggccgg tccagccgcc agcacgctct ggcaacggct
     2041 tgcggtggac catgagcgac tggtggcact tgccacccgg ctcgttgcgg atggcggcgc
     2101 tcaggctgcc gaagcgacgc ttcacctgct cgttctggac caactggatc cgtatggaat
     2161 cggcgttgtc gcccgccagc agatcgcatc cagcgcgctc ggatcatccg cgccggtcgt
     2221 gggcgggcgt
//
LOCUS       DASUGJ010000517         4764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1471076, whole genome shotgun sequence.
ACCESSION   DASUGJ010000517 DASUGJ010000000
VERSION     DASUGJ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471076"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFV93_11635"
     CDS             complement(<1..429)
                     /locus_tag="VFV93_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5166040.1"
                     /translation="MDAEQFDVVIVGAGPAGCVLAARLTEDGDRRVLLLDAGPDYGSD
                     AAAWPEDLLDPYHSAVLSHSWGYVNAPTAAGFSVPLPRARVVGGCSAVNACIWLRGSA
                     ADYDYWAAQAGPQWSWEAMRAAFRRSESDTSGNPGMHGSDG"
     gene            579..1565
                     /locus_tag="VFV93_11640"
     CDS             579..1565
                     /locus_tag="VFV93_11640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5166041.1"
                     /translation="MTALPNGYRLRPVTLDDLDSVVDLMNRASIADIGTGGVTASRLR
                     TFWLGPDRNLATDNWIVEDAGGEAVAYMDFNEYAPYDLSEFYDVIDPEHRGRGIEEAL
                     IAVASERASTSIAKASPEATVRLETDAWASDPVDVERHLSLGFSLTRVWNRMQIEMTD
                     FPPAPAWPKGISVRTFQPDEVLDVHQAWEDAQRDEWGFSSLTPDEFRYYFVENEENFD
                     PTLWFLAIDDTTGTIAGYALCRWERPGVPECGHIRYVAVRRQYRRRGIAQALLLHTFR
                     EFYARGKRQVGLAVDSTSLTGADRLYERVGMRPIQQSVVFSTVLREGAGETN"
     gene            1700..2242
                     /locus_tag="VFV93_11645"
     CDS             1700..2242
                     /locus_tag="VFV93_11645"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX5166042.1"
                     /translation="MAKRKDRTYEGVVVQNFRTEDQAIDAIRRLQSAGFTNDQVSVIA
                     RDKDVSARVTDDTGTEAEEGAIAGAVTGGVLGGVAALIAGASAVAIPGVGLAIGAPIA
                     AAIAGAAGGGLVGGLVGMGIPEEDARVYNERLEQGDIIVSVIAGSRESEARQILQGDG
                     VIGTHLHTAGTSATRPDVIS"
     gene            2430..3023
                     /locus_tag="VFV93_11650"
     CDS             2430..3023
                     /locus_tag="VFV93_11650"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEX5166043.1"
                     /translation="MATLAESLLQQINAAQASSGGSAVNGDSATIQSGLAAAIPALIT
                     ALGRNAQSKDGAAALHDAIAKDHDGSVIDNLGSYLQNPDLDDGDGILEHALGNQREPV
                     AAGISQQTGLDMNTIAKLLQMAAPLVLGMLAKQQQQKNLSANDLAGLLDEERQQEAQA
                     NPDLMQMVSGLLDQNDDGSVMDEVQSLAGKLFGRKRD"
     gene            3080..3283
                     /locus_tag="VFV93_11655"
     CDS             3080..3283
                     /locus_tag="VFV93_11655"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX5166044.1"
                     /translation="MSVAKVVEILASSPNGFDEAVQNGIERASKTLEGIKGAWVKDQQ
                     VVVENGKITEYRVALKVTFVLHD"
     gene            3449..>4764
                     /locus_tag="VFV93_11660"
     CDS             3449..>4764
                     /locus_tag="VFV93_11660"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5166045.1"
                     /translation="MSTTIEGALSAQIDDIFSEWDRIDSPGMAVAVIHNGEIIHQRGY
                     GMSNLDHAIPIESDSVFHVASVSKQFTAFAIALLAHDGKLSLDDDLRTHVPELPDLGQ
                     TITIRHCIHHTSGLRDQYGLFRLAGWRDDDTQTLADVLDFAYRHERLNFTPGDEYRYC
                     NTSYTLLALIVERVSGQSFRSFVNDRLLAPLGMTDSHIHDDHTAIVPRRTSAYNTRDD
                     GSLKVANSTVSAVGAICLFTTAEDLAKWVRNYQTRDVAGDILDAAFTSGALNSGHPTH
                     YGYGLALSTYRGLRTIGHGGSDSGYRAQVTWFPDADLGVVILANLATIKPGPLALRIA
                     DIVIPDQLGADDLADASAIDLDPAALKPLTGVYHTTPSQQIREVTLDDNTLHITADFG
                     DKQPMTPLGHRRFRADDPPFEVTFTGPDTALELHITDRNGDTRSFTR"
BASE COUNT          918 a         1829 c         1293 g          724 t
ORIGIN      
        1 tccgtctgaa ccatgcatac cgggattgcc gctcgtgtca ctttccgaac gccggaacgc
       61 cgcacgcatc gcctcccaac tccactgggg accggcctga gccgcccaat agtcgtagtc
      121 ggcggctgac ccgcgcagcc agatgcaggc attgacggcc gagcatccgc caacgactct
      181 ggctcgcggc agcggcacgc tgaaacctgc cgccgtcggc gcgttcacat agccccacga
      241 gtgcgagaga acggcgctgt ggtacggatc cagcagatcc tcgggccacg ctgcggcgtc
      301 cgacccgtag tccggtcccg cgtcgagcag cagcacgcgc cggtcgccgt cctccgtcaa
      361 ccgcgcggcc agcacacagc cggctggacc cgccccaacg atcacgacgt cgaactgctc
      421 cgcgtccatc agccatactc ctcaatcgcg cactcacggc gcgacggatc gtacgtcgcc
      481 tgctcgaccc cgtcaactcg cggacgcagc ccgtgtggca tcatctctcg cctgcccggc
      541 tcaacggcga accggggtcg cgcgcgaagg acacgacgat gactgccctc ccgaatggat
      601 accgtctccg ccccgtcacg ctggacgatc tcgactccgt cgtcgacctc atgaatcgag
      661 catcgatcgc cgatatcggc accggcggcg tgaccgcgtc ccgcctgcgc accttctggc
      721 tcggacccga tcgcaacctc gccaccgaca actggatcgt cgaagacgcc ggcggcgagg
      781 ccgttgcgta catggacttc aacgagtacg caccctacga cctcagcgag ttctatgatg
      841 tcatcgaccc cgagcaccga gggcgtggca tcgaagaggc gctcatcgcc gtcgcctccg
      901 agcgtgcatc aacgtcgatc gccaaggcct cgcccgaagc caccgtccgg ctcgagacgg
      961 acgcctgggc gtccgatccc gtcgatgtcg agcgccatct cagcctcgga ttctccctca
     1021 cccgtgtctg gaatcggatg cagatcgaga tgaccgactt cccgcccgcc cccgcctggc
     1081 cgaaaggcat cagcgtccga accttccaac cggacgaggt tctggacgtc caccaggcat
     1141 gggaagacgc ccagcgcgac gagtggggct tctccagtct caccccggac gagttccgtt
     1201 actacttcgt cgagaacgaa gagaatttcg atccgactct ctggttcctc gccatcgacg
     1261 acaccaccgg cacgatcgcc ggctacgcgc tctgtcgctg ggaacgtccc ggcgtgccgg
     1321 agtgcggcca tatccgctat gtcgccgtcc gtcgccaata ccgccgtcgt ggcatcgccc
     1381 aggcgttgct actgcacacc ttccgcgagt tctacgcacg cggcaagcgg caggtcggac
     1441 tcgccgtcga ctcgaccagc ctgaccggcg ccgaccgcct ctacgagcgc gtcggtatgc
     1501 gcccgatcca gcaatcagtc gtcttctcca cagtactccg cgagggcgct ggcgaaacga
     1561 actgacgatc gcgacgacga ttacagcgta cgtacgcgac cccgctatcg cgtttccctg
     1621 ttcccaacgg tcacagtggg cacgccacgt gcgtatccca ccgtcggtaa ccgaacaggg
     1681 aatgaaggga tatcaatcaa tggctaagcg caaagacaga acctacgaag gcgtcgtggt
     1741 ccagaacttc cggaccgaag atcaggcgat cgacgcgatc cgccggctcc agtcggcagg
     1801 cttcaccaac gatcaggtga gcgttatcgc ccgtgacaag gatgtctcag ctcgcgtcac
     1861 ggacgacacc ggcaccgagg ccgaagaggg tgccatcgcc ggcgccgtca ccggtggcgt
     1921 gctcggtgga gtcgccgccc tgatcgcggg cgcatcggca gtcgcaattc caggtgtcgg
     1981 gctcgcaatc ggtgctccaa tcgccgcagc catcgccggc gccgctggtg gcggtctcgt
     2041 cggcggactt gtcggcatgg gtatcccgga ggaagacgct cgcgtctaca acgagcgact
     2101 tgagcaaggt gacatcatcg tctccgtcat cgccggttca cgcgagagcg aggcacgcca
     2161 gatcctgcaa ggtgatggtg tcatcggcac tcacctgcac actgccggca ccagcgcaac
     2221 ccgcccggac gtcatcagct aactgagcgt ccaccggctt caatgcgagg gacgggcaga
     2281 tgcccgtctc tcgtcatgct cgcgcgtcct ctcaaacgca attcgcagcc ggcctccctg
     2341 acacttgcga acgtcgccaa tgtgcgtcat gctgtctcaa ccgtccacat gtcgcggcga
     2401 cgccgagcac aaggaatgga gacagtgcca tggcaactct ggcggaatcg ctactccagc
     2461 agatcaacgc agcccaggca tcaagcggtg gcagcgccgt caacggcgac agcgccacga
     2521 tccagagcgg gctcgctgcc gcgatcccgg cactgatcac cgccctgggt cgcaatgctc
     2581 aatcgaagga cggagccgcc gcccttcacg acgcaatcgc gaaagaccac gacggtagcg
     2641 tcatcgacaa tctcggctcc tacctgcaga accccgacct cgacgacggc gacggcattc
     2701 tcgagcacgc cctcggcaac cagcgcgaac cggtcgcggc cggcatctcc cagcaaaccg
     2761 gcctcgacat gaacacgatc gccaagctgc tgcagatggc cgccccgctc gtcctcggga
     2821 tgctcgcgaa gcagcagcaa cagaagaacc tcagcgccaa cgacctggcc ggcctgcttg
     2881 acgaggagcg ccagcaggaa gcgcaggcca accccgacct gatgcagatg gtgtccggcc
     2941 tgctggatca gaacgacgac ggcagcgtca tggacgaagt ccagagcctg gccggcaaac
     3001 tcttcggccg caaacgcgac tgagcaacat cgttccatca ggcatccgtt taccgtctct
     3061 accagaaagg cagcatctca tgtcggttgc gaaagttgtc gagatcctcg cctcatcacc
     3121 aaacggcttc gacgaagccg tccagaacgg gatcgagcgc gcctcgaaga ccctcgaagg
     3181 catcaagggc gcctgggtca aggaccagca ggtcgtcgtc gagaacggca agatcaccga
     3241 gtaccgcgtc gccctgaagg tgacgttcgt cctccacgac tagccggcgc caccagcgca
     3301 ggttccaacg cgaccgagcg ttgcccggtc gcgttgcttt cctctcgaat cgcccaactc
     3361 ccacgctcaa ccacagacaa ccggcccgca aacggcgata ctagcccgcg aaagccgatt
     3421 gcctggtatg agatgggaca gttcacacgt gtcaacaaca atcgagggtg ccctcagcgc
     3481 ccagatcgac gacatcttct ccgagtggga ccgcatcgac agccccggca tggccgtcgc
     3541 cgtcatccac aacggcgaga tcattcacca gcgcggctac ggtatgtcca acctcgacca
     3601 cgccatcccg atcgagtccg actccgtctt tcacgtcgcc tccgtctcga agcagttcac
     3661 cgcgttcgcc atcgccctcc tcgcccacga cggcaagctc tcgctcgacg acgacctccg
     3721 cacgcacgtc cctgagctgc ccgatctcgg ccagaccatc acgatccgcc actgcatcca
     3781 ccacaccagc ggcctgcgcg accagtacgg cctcttccgc ctggccggct ggcgcgacga
     3841 cgacacccag accctcgccg acgttctcga cttcgcctac cgccacgagc gcctcaactt
     3901 cacccccggc gacgagtacc gctactgcaa caccagctac accttgctgg ccctcatcgt
     3961 cgagcgcgtc agcggccagt cgttccgctc cttcgtcaac gaccgcctcc tcgccccgct
     4021 cggcatgacc gacagccaca tccacgacga ccacaccgcc atcgtccccc gccgcaccag
     4081 cgcctacaac acccgcgacg acggcagcct caaggtcgcc aactccaccg tctccgccgt
     4141 cggcgccatc tgcctcttca cgaccgccga agaccttgcc aaatgggtcc gcaactacca
     4201 gacgcgcgac gtcgccggcg acatcctcga cgccgccttc acgtccggcg ccctcaacag
     4261 cggccacccg actcactacg gctacggcct cgccctcagc acctaccgcg gcctgcgcac
     4321 gatcggccac ggcggcagcg actccggcta ccgcgcccag gtcacctggt tccccgacgc
     4381 cgacctcggc gtcgtcatcc tcgccaacct cgccaccatc aagcccggcc cgctcgccct
     4441 ccgcatcgcc gacatcgtca tccccgacca gctcggcgcc gacgacctcg ccgacgcctc
     4501 cgccatcgac ctcgaccccg ccgccctcaa acccctcacc ggcgtctacc acaccacccc
     4561 gtcacagcag atccgcgagg tcaccctcga tgacaacacg ctccacatca ccgccgactt
     4621 cggcgacaag cagccgatga ccccactcgg ccaccgtcgc ttccgcgccg acgatccacc
     4681 cttcgaagtc accttcaccg gccctgacac cgccctcgaa ctccacatta ccgaccgcaa
     4741 cggcgacacc cgctccttta cccg
//
LOCUS       DASUGJ010000518         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1749590, whole genome shotgun sequence.
ACCESSION   DASUGJ010000518 DASUGJ010000000
VERSION     DASUGJ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749590"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1046)
                     /locus_tag="VFV93_11665"
     CDS             complement(204..1046)
                     /locus_tag="VFV93_11665"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5166046.1"
                     /translation="MADRSAAQHDPETWTGGMNLRAAMDQHDRARQTAEDTTIDWLPV
                     IGGGLLAGAGVGRGGGRGLLMTLLGGGLVYWGMSRGVAALPRPSGRSLAPDGERSVRV
                     ERVITINRPVAEVYAAWRDVESLPSIMSHLESVTRTDDGLTHWVARAPLGRTVEWDAE
                     IINDEAGRVISWRSVEGASVPNVGAVHFDEAPAGRGTELRVRIEYNPPAGALGATIAS
                     LFGEEPSQQVADDLRRFKSFMETGEYPTTDGQPRGAHQQSIMDPILHKVSDVTDAAGT
                     ADSR"
     gene            1233..2018
                     /gene="otnI"
                     /locus_tag="VFV93_11670"
     CDS             1233..2018
                     /gene="otnI"
                     /locus_tag="VFV93_11670"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF043033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEX5166047.1"
                     /translation="MPRFAANLTMLFTEYPFIERFDRAAAAGFEAVEFLFPYAEDVNA
                     IREALTRNGLKQVLFNLPAGDFAAGERGFANDPARINEFRDGVNRALEIAATLECGQI
                     NCLIGLNREDIPLDTQWETVSGNLYYAAETAKGAGVRQLIEPLNTIDTPGFLLSTTDR
                     AVELIREVASDNLFVQYDVYHAQRTEGNIAATIDRQVAAGAIKHIQIADSPARNEPGT
                     GELHWPFIFAAIDASGYDGWVSAEYRPKGKTEDGLGWMGGTDV"
     gene            2011..>2900
                     /locus_tag="VFV93_11675"
     CDS             2011..>2900
                     /locus_tag="VFV93_11675"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR01505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEX5166048.1"
                     /translation="MSERIGFIGLGIMGRPMARNLVKAGYELVVHSRSRGPVDELVDE
                     SGAVTAAGSPREVAEQAQIVITMLPDSPDVHAVVFGEDGLLGAMGAGSLLVDMSTIAP
                     ATAIEVEAALRAKGAGAVDAPVSGGEPGAINAALSIMAGGSEADFERAKPLFEALGKT
                     IVHVGGPGAGQTVKACNQIVVAITYAAVSEALVLGSKAGVDPAKIVQVLSGGLAGNKI
                     LELKGKGMVEHAFQPGFRINLHRKDLAIATASGKEFGVPLPVTALVTQMFEALAVAGR
                     GDLDHSAYLTLVEDMAAHQIG"
BASE COUNT          481 a          884 c         1052 g          483 t
ORIGIN      
        1 atacggacga tcgcgtcgcg aggattcagg atcgtcggat cggggacgtt ctcgacacgg
       61 acatcgttct taccgtgcca ggtcagtgcg cgcatggtgg gggtgctccg ttctggggaa
      121 cgggattcgg gattcgggat ccggggtgtc gagcggtaga gttcgcgtcg atgcccccga
      181 atcccaaatc ccgaatcccg atatcaacgg ctatcagcag tgccagcagc gtccgtcaca
      241 tcactcacct tgtggagaat cgggtccatg atgctctgct ggtgggcgcc gcggggttgg
      301 ccatcggtgg tggggtattc gccggtctcc atgaaggact tgaagcgacg caggtcgtcg
      361 gcgacctgtt gggagggttc ctcgccgaag aggctggcga tggttgcgcc gagcgcgcca
      421 gccggtgggt tgtattcgat ccggacgcgt agctcggtgc cacggccggc cggtgcctcg
      481 tcgaagtgga cggcgccgac gttcgggacc gacgcgcctt cgaccgagcg ccaggagatc
      541 acgcggccag cctcgtcgtt gatgatctcg gcgtcccact cgacggtgcg gcccagcggc
      601 gccctggcga cccagtgggt cagtccgtcg tcggtgcggg tgaccgattc gaggtggctc
      661 atgatcgacg gtagcgactc gacatcgcgc caggcggcgt agacctcggc caccggccgg
      721 ttgatagtga tgacccgctc gacgcgaacg ctgcgctcgc catcgggcgc cagcgagcgg
      781 cccgacgggc gcggcaacgc ggcgacacca cggctcatgc cccagtagac gaggccgcct
      841 ccgaggagcg tcatcagcag cccgcgacct ccgccgcgac cgactccagc gccagccagc
      901 agcccgccac cgatgaccgg cagccagtcg atcgtggtgt cctcggccgt ttgccgggcg
      961 cggtcgtgct ggtccatcgc ggcgcggaga ttcattccac cggtccaggt ctcgggatcg
     1021 tgttgtgctg ctgatctgtc cgccattgtc gtcgtctccc tgctggtctg ctcttcatgt
     1081 acacgatggc agaggcgcaa ccggcgtgcc gcggtggatc gggaatcggg gatgaggaat
     1141 gtcggacggt gacgaatcat tgacccgtgc ttgcggacgg cttagcatgg cggcggccgg
     1201 aggggtgtgc tggttggagg gacggagttg cgatgccgcg ctttgccgcg aacctgacga
     1261 tgctgtttac cgagtaccca ttcatcgagc gtttcgaccg ggcggcagcg gccggcttcg
     1321 aggcggtcga gtttctgttc ccgtatgcgg aggatgtcaa cgcgatccgg gaagcgttga
     1381 cccggaacgg gctgaagcag gtgctgttca acctgccggc gggagatttc gcggccggtg
     1441 agcgggggtt cgccaacgac ccggcccgca tcaacgagtt ccgcgacggg gtcaaccggg
     1501 cgctggagat cgcagcgacg ctcgagtgcg gacagatcaa ctgcctgatc gggctgaacc
     1561 gtgaggacat cccgctcgac acgcagtggg agacggtctc cggcaacctc tactacgctg
     1621 ccgagacggc caagggggcc ggcgttcgcc agctgatcga gccgctcaac acgatcgaca
     1681 cgcccggttt cctgctctcg acgaccgatc gggcggtcga gctgatccgc gaggtcgcca
     1741 gcgacaacct gttcgtgcag tacgacgtct accacgccca gcgaacggag gggaacatcg
     1801 ccgcgacgat cgaccggcag gttgcggctg gcgcgatcaa gcacatccag atcgccgaca
     1861 gcccggcccg caacgaaccc ggcaccggcg agctgcactg gccgttcatc ttcgcggcga
     1921 tcgacgccag tggctacgac ggctgggtca gtgccgagta ccggccaaag gggaagaccg
     1981 aggacgggtt ggggtggatg ggaggaacag atgtctgaac ggatcggatt catcggcctt
     2041 ggaatcatgg gccggccgat ggcgcgcaac ctggtcaagg cgggttacga gctggtcgtg
     2101 catagccgca gccgggggcc agtcgatgaa ctggtcgacg agtcgggtgc ggtgacggcg
     2161 gccggcagcc cgcgcgaggt ggccgagcag gcgcagatcg tgatcacgat gctgcctgat
     2221 tcgccggacg tgcatgcggt cgtcttcggc gaggatgggc tgctgggagc gatgggtgcc
     2281 ggtagcctgc tggtggacat gagcacgatc gcaccagcga cggcgattga ggtcgaggcg
     2341 gcgctgcggg cgaagggcgc cggggcggtc gatgcaccgg tctccggtgg cgagccgggc
     2401 gcgatcaacg cggccctctc gatcatggcc ggcggcagcg aggcggattt cgagcgagcg
     2461 aagccactct tcgaagcgct gggcaagacg atcgtccacg tcggcggccc gggcgcgggg
     2521 cagacggtca aggcctgcaa ccagatcgtc gtcgcgatca cctacgcagc cgtcagtgag
     2581 gcgctggtgc tcggctcgaa ggcgggcgtc gatccggcca agattgttca ggtgctttcg
     2641 ggcgggctgg ccggcaacaa gatcctggag ctcaagggca aggggatggt cgagcacgcc
     2701 ttccagcccg gattccggat caacctgcac cgcaaggacc tggcgatcgc aactgccagc
     2761 ggcaaggagt tcggcgtccc gctgccggtg acggcgctgg tcacgcagat gttcgaggca
     2821 ttggcggtgg caggccgtgg ggatctcgac cactcagcct atctcacgct cgttgaagat
     2881 atggctgcgc accagatcgg
//
LOCUS       DASUGJ010000519        13062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1612280, whole genome shotgun sequence.
ACCESSION   DASUGJ010000519 DASUGJ010000000
VERSION     DASUGJ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13062
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612280"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..940)
                     /locus_tag="VFV93_11680"
     CDS             complement(161..940)
                     /locus_tag="VFV93_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166049.1"
                     /translation="MLDFETRRRPAVTSDETFPSTPAVVNVQSLSYAYPNGHVALREV
                     SLTIHEGERVALMGPNGAGKSTLLMHLNGIVQSPRPRGRIEICGQPLNDETIRRIRAL
                     VGVVFQNPDDQLFSPTVYDDVAYGPLYMGLPKDEIRQRVERSLAAVDMAGFETRLPHQ
                     LSLGQRKRISLATVLAMDPRILVLDEPSAGLDPRARRGLIALLDGLPQTLIASTHDMR
                     LVRDLFTRAVIMQEGRIVIDAPTSDIIDDESLLEAYGLELP"
     gene            complement(927..1718)
                     /gene="cbiQ"
                     /locus_tag="VFV93_11685"
     CDS             complement(927..1718)
                     /gene="cbiQ"
                     /locus_tag="VFV93_11685"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEX5166050.1"
                     /translation="MSVFVLGERYHHGTGLLYRADPRVKILIAVVFAFAVTAIPEGHW
                     YAFVGFGAMVLLAIALSKLPPLLVFRRALIALPFVAAAVPLIFTRPGETVFTVPLLGW
                     TASDVGLTAVASIMLKSWLAVLMAVILTSCTQPIDLIRGLERLRVPRVLASTIFFMYR
                     YLHVIGGEGHRMMRARDSRSAAASAGANSGGSIAWRARVLGNMIGSLFLRSFERSERI
                     HAAMQARGYDGTIRFADDRALVRADWLLLVSALTLLGGLVLYARL"
     gene            complement(1725..2072)
                     /locus_tag="VFV93_11690"
     CDS             complement(1725..2072)
                     /locus_tag="VFV93_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HEX5166051.1"
                     /translation="MKNRRNWGILIAAGVGLALLITLFSPFASSNPDGLEKVAEDKEF
                     SHHAEGASYEIIPDYTFPGVENERLATILSGIVGVLIVAGIGLGAGYGLRALARSRAD
                     DNPPSTPQSTRAG"
     gene            complement(2074..2784)
                     /locus_tag="VFV93_11695"
     CDS             complement(2074..2784)
                     /locus_tag="VFV93_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX5166052.1"
                     /translation="MQIPAIPILMHIPDGFLSTPVAAVFYVLTGIVVGMAVRQTNRTL
                     KESTAPLMGVLAAFIFAAQMMNFPVAGGTSGHMLGGALAAILLGPWAAVIVMTSVIGI
                     QALVFQDGGLAALGANIFNMGITTAFVGYAVYKTVAATGRYSRPFILGGTFVAAWISV
                     MLAALLTALQLVISDTSEMSVVVPAMLGVHALIGIGEGLISVGAVALVLSARPDLLNV
                     APEETEPIGYSALSGESS"
     gene            3083..3601
                     /locus_tag="VFV93_11700"
     CDS             3083..3601
                     /locus_tag="VFV93_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166053.1"
                     /translation="MIAKPMLTRESLEHFGNLPEAYQHEWRVATPLEPIVVPEGVFKW
                     YHVHRDGVEVDESLDRAARELICDGAMTWNLEYGLNFALLHQSHERAFLIIGVWRGHQ
                     ELWSRAYEISLLDDDSFRRIEATEEFWPTACVWEMGVIQHERMAWHRYLFTEHTDADK
                     LAWLDDRYAGMV"
     gene            3637..4188
                     /locus_tag="VFV93_11705"
     CDS             3637..4188
                     /locus_tag="VFV93_11705"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5166054.1"
                     /translation="MARFDPGRPAGDEHLPYWGQYIELVPDGDIADVLESQISTTVDS
                     LRALTPVQAGRRPAPGEWNALEIVGHLADTERVLAYRVLRIARADPVLWESIEFEPYV
                     EHGEFDGRELWTLIDEFVATRAATVAMLRGLGEAAWQRRMPEEWSCRSVRSLAYVIAG
                     HELHHLADIRGKELAAQAGVRQI"
     gene            4302..5024
                     /locus_tag="VFV93_11710"
     CDS             4302..5024
                     /locus_tag="VFV93_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5166055.1"
                     /translation="MADAGTIRHFEALGVDDGWNCLEAGAGGGSMTAWLCDRVGPDGS
                     VVAVDINTRFVEDLDRPNLEVRQLNVVDEALPAGQFDLVHTRAVLAHLPNREPAIDNL
                     TNALAPNGWLLAEEMDFLSVAPVPGVDPAQADRFMKLVDAHHQALAGKMDPFFGRRVT
                     DALCQTGLDDIGSEGRTTIVRGGSPGATAWALTFEQLSETMLEHGWVRETELAEVYDA
                     LEDPSFAFQSMVFMAAWGRKRG"
     gene            complement(5109..6161)
                     /locus_tag="VFV93_11715"
     CDS             complement(5109..6161)
                     /locus_tag="VFV93_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEX5166056.1"
                     /translation="MEATDLREAPITIVGAGAIGGTVGAFLTDAGYDVTLVDVVPEHV
                     QIMNERGLRITGLRGDRTFPVKAVLANDLRGPLGVTFLCVKGHFTEPAMQQVGPLLAP
                     DGYILSLQNGLNEEIIARHVGRERTVGAFVHFGADYMEPGLIQLGNEQTIHPGELDGT
                     ITSRLRALETALSHVMPVELTTNIWGYLWGKLVYGAMAFAVSCVDAPVPTVIDNPLGR
                     RICQAASAEAYLVAKSQADHLEWIGEFDPDAFAPGDDFEQRSNAALDELADAMRASIK
                     QHMGIWRDLKIKKRKTEVDMQSAVIVEKGAANSIPTPVNAAVLQVIHEIEDGQRDMDW
                     TNLDDIARMAALHDAV"
     gene            6248..6625
                     /locus_tag="VFV93_11720"
     CDS             6248..6625
                     /locus_tag="VFV93_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5166057.1"
                     /translation="MRYGLHHVSIPVRPGQLEAGREFYGQALGLEEIPVPAEFEEGRV
                     VWFSLGDRELHLFKEEDANQPRSERHLALEVDDLAAARARFAEHGIAVEETDPIHNRP
                     RLYIRDPFGNKLEITELQGPYRE"
     gene            7211..8200
                     /locus_tag="VFV93_11725"
     CDS             7211..8200
                     /locus_tag="VFV93_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5166058.1"
                     /translation="MTERAIQDTYELERLAADAEVGDVIDESAELIDQATSDAVYRYF
                     RDVGGHRLLSHEEEIDLSRKVRAGLEVRRRREAGEDVENLQQTLELARGAREKLIRHN
                     LRLVVSIAKKYRSSGLPLIDLIQEGNIGLMTAVERYDPELGYRFSTYATFWIRQAIGR
                     AVANLSRTIRVPVHMHDLISKVRRTESQLEQQYGRPATDAEVAAHLEMEVERVEQARS
                     AIPRTSSLDKPIGEDGESTIGDLLPDPASDQVVEEAVSSAIRDQIRKSLDALTERERG
                     VLMLRFGLNGQQPQTLAEIADHYGISRERVRQIEKEALAKLRKSDLIELANAA"
     gene            8339..8914
                     /locus_tag="VFV93_11730"
     CDS             8339..8914
                     /locus_tag="VFV93_11730"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5166059.1"
                     /translation="MIIETPPYMPLGVREYLELEEASSVRHEYVGGILHALAGASRRH
                     NQIITNLITDLGPIANQANCTVHHETVKLQVADDTIYYPDFMIVCHLDDTDAFVVERP
                     CVVVEVVSSSSRVIDRREKVMAYRRVPSLLAYLVIEQDEPSIQRHWRTPEGAWLHGTI
                     AREGIVPIPCLGTELSFEQIYRRIDFSTSSG"
     gene            complement(8878..10242)
                     /locus_tag="VFV93_11735"
     CDS             complement(8878..10242)
                     /locus_tag="VFV93_11735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166060.1"
                     /translation="MHDQHQSTGGPSTPRWPWLQPDLGLGRNNGILFWVHLIWGAGFT
                     IQAALWTLFIESLGATPTQIGLVLGGGAVVRTILAIPAGHLADRFPLKPVIIAMMAVP
                     IFGAISLALATQWWHALAGAVLMDMSGVAIPAVSAYVAAASKPEDRTRTMTYIFTLSW
                     LISMTVAPAIGGWLAETSGFRTVYLVGAALFAAGIVVATRIDNIRPSQEPAETGSAAQ
                     PIGYRDLLRSTGVHVILGFHLLVPLLVVIGFALMPNFMNDDRGVSLSTIGVLASIGAV
                     LAFGMSLLVSHWKPLTSPFMGMAAILSIAALSMALMLSFTALPIVAFAFMLRMCFSPI
                     WSLMASAVAEVTPERVRGRAYGLCEFVVGIGDTTAPIASGRLYATDHRLPLIVGLVTT
                     APLALASLVAHRLRGRFGMKSTVSDDRPSSETLQRDIDPTVDSLSADKIAVHPELVEK
                     SIRR"
     gene            complement(10527..10973)
                     /locus_tag="VFV93_11740"
     CDS             complement(10527..10973)
                     /locus_tag="VFV93_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166061.1"
                     /translation="MSDVRDTRQRIQDVRTLLRSERDAWVPSANGSGDAYIIPLSFSW
                     DGELVTMATPRSSQTARNLRRAGWARIALGSPLDVVIIEGTLSQILASDAGSFADEHA
                     QAVGFDAREQTQEYVFFRLTPERIQAWRSPAELPARDVMRAGRWLA"
     gene            complement(10970..11491)
                     /locus_tag="VFV93_11745"
     CDS             complement(10970..11491)
                     /locus_tag="VFV93_11745"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5166062.1"
                     /translation="MQETPEDLERLSTLLDDSIKRAGAFLRSSFEMPEKSLSARQLVT
                     YLQGIQTVAFATVTAAGEPRVAPIGSLFYRGAFYIPTVTTAARLRHIQRRPAVSLTYY
                     QGNDLAVIVHGQASLLTSDDPTFATLEALHRSFGSSSVNDWGDGAFIHVEAASIFTYA
                     RFPEHFPERQACA"
     gene            complement(11576..12433)
                     /locus_tag="VFV93_11750"
     CDS             complement(11576..12433)
                     /locus_tag="VFV93_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166063.1"
                     /translation="MLPSARASSISSSAIDRDRPTRLDALRHELASVIASWRAATGEW
                     LYHMGRGGLIQMMIIPITQISIFALIYGTSSDLLGYLVVAQAAGAFIMTMIYYNGEIL
                     DREREKGTLVSLFLAPCSRAAWLSGFILAGLIQTISMLTTSLLFGWLAFGVTLDPNLP
                     ALAVSLLLFMAALWGLGLIFAGIGLILKRANAFSNLIWPFVVLLGGLYFPVSELPTGM
                     RQLARVLPFGYGIQAIADASLHHASLTQIQSDLIPLAGFAIALPLAGILAFAWLERLV
                     RIRGELDLY"
     gene            complement(12396..>13062)
                     /locus_tag="VFV93_11755"
     CDS             complement(12396..>13062)
                     /locus_tag="VFV93_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166064.1"
                     /translation="KASGATTIDGVNISGFLLAGMVGMICWYSSVWWTGSAIEEERWE
                     GTIAALFLTPASRVAVVIGHGLGGFIYMIPSFATIVLLAVITGARFNVGSLVAVVASA
                     LALLVASLAMGFLLASLFILSRRANLMANIIQHPLYLMGGFMIPRDALPDWLHTISNA
                     MPLAHAVDAFRASMLASASLHDVGNSLLAAALTSSLFVLTGLLGIRRVEDVAKRAGQL
                     DLF"
BASE COUNT         2499 a         3770 c         4654 g         2139 t
ORIGIN      
        1 gttagttcag agtcaacaag ggcgggattc gggattcggg acgcgggacg tgggacgcgg
       61 gatgtgtcgc aaccacgcga cgcaccgagg atcgtcacgc cttgccctgt catcctgagc
      121 gccagcgaag gatctcgttg cccgttggcg cgacgagtgg tcaggggagc tcaaggccgt
      181 atgcttcgag gagagattcg tcgtcgatga tgtcggaagt gggtgcgtcg atgacgattc
      241 ggccttcctg catgatcacg gcgcgggtga agaggtcgcg gacgaggcgc atgtcgtggg
      301 tggaggcgat cagcgtctgc gggaggccgt cgagcaatgc gatcaggccg cggcgggcgc
      361 gggggtcgag tccggcggat ggttcgtcga ggacgaggat gcgggggtcc atcgccagga
      421 cggtcgcgag gctgatgcgc ttgcgctggc cgagcgagag ctggtggggc aggcgcgtct
      481 cgaatccggc catgtcgacg gcggcgaggg accgttcgac gcgctgccgg atctcgtcct
      541 tcggcaaccc catgtagagc gggccgtagg cgacatcgtc gtagacggtc ggcgagaaga
      601 gctgatcgtc agggttctgg aagacgacgc cgacgagcgc gcgaatccgg cggatcgtct
      661 cgtcgttgag tggctggccg cagatctcga ttcgaccacg tgggcggggc gactggacga
      721 tgccgttgag gtgcatcaac agcgtggact tgccggcgcc gttgggaccc atcaatgcga
      781 cgcgttcacc ctcgtggatg gtcaggctga cctcgcggag ggcgacgtgg ccgttcgggt
      841 aggcgtacga gagcgactga acgttgacga cggccggggt ggatgggaag gtctcatcac
      901 tcgtcacggc cggccggcgg cgagtctcaa agtcgagcat acagtacgag acctccaagc
      961 agggtcaggg cgctgacgag aagcagccag tcggcgcgaa cgagcgcgcg gtcgtcggca
     1021 aagcggattg tgccatcgta gccacgtgcc tgcatggcgg cgtggatgcg ttcgctgcgt
     1081 tcgaacgatc gcaggaagag cgaaccgatc atgttgccca gcacacgtgc ccgccaggcg
     1141 atgctgccac cggaattggc tccggccgat gcggcggcgc tgcgggagtc gcgcgcgcgc
     1201 atcatgcggt ggccctcgcc gccgatgacg tgcaggtagc ggtacatgaa gaagatggtc
     1261 gaggccagca cgcgggggac acgcagccgc tccagaccgc ggatcaggtc aatcggctgg
     1321 gtgcaggagg tcaggatcac ggccatcagg acagccagcc acgacttgag catgatgctg
     1381 gcgacggccg tcaggccaac gtccgaggcg gtccagccca ggagcgggac ggtgaagaca
     1441 gtctcgccgg ggcgggtgaa gatgagtggg acggcagcgg ccacgaacgg cagcgcgatc
     1501 agcgcgcggc ggaagacgag cagcggcggc agcttgctga gggcaatcgc cagcagcacc
     1561 atcgcgccaa agccgacgaa ggcgtaccag tggccctccg gaatagccgt gacggcgaac
     1621 gcgaatacga ccgcgatcag gattttgacg cgcgggtcgg cgcgatagag cagcccggtg
     1681 ccgtggtggt agcgttcacc gaggacgaaa accgacacgg gcggctaacc tgcccgggtt
     1741 gactgcggcg tcgacggcgg gttgtcgtcg gcgcgcgaac gtgccagcgc ccgcaagccg
     1801 tagccagcac cgagaccgat gccggccacg atcaggacgc caacgatgcc ggaaaggatc
     1861 gtcgccaacc gttcgttctc gacaccgggg aaggtgtagt cagggatgat ctcgtaactc
     1921 gccccttcgg cgtggtggct gaactccttg tcctcggcga ccttttcgag gccgtcaggg
     1981 ttgctggacg caaacgggct gaagagcgtg atgagcaggg caaggccaac gccggcagcg
     2041 atcaggatgc cccagttgcg gcggttcttc atcttaggaa ctctctccag acaaggccga
     2101 atagccgatc ggttccgtct cctccggcgc gacgttgagc aggtccggcc gggcagagag
     2161 cactagcgcg acggcgccga cgctgatcag cccctcgccg atgccgatca gggcatggac
     2221 gccgagcatg gccggaacaa cgacgctcat ttcggatgtg tcggagatta cgagctgaag
     2281 tgcggtcagc aaggccgcca gcatgacgct gatccaggca gcaacgaagg tgccgccgag
     2341 gatgaacggc cggctgtagc gtccagtcgc ggcgactgtc ttgtagacgg cgtagccgac
     2401 gaaggcggtc gtgatgccca tgttgaagat gttggcgccg agggcggcca gcccgccgtc
     2461 ctggaagacg agtgcctgga tgccgatgac ggaggtcatg acgatgacgg ccgcccaggg
     2521 gccgagcagg atcgccgcca gcgcaccgcc cagcatgtgg ccggacgtgc cgcctgcgac
     2581 cgggaagttc atcatctggg cggcgaagat gaacgccgcc aggacgccca tcagtggggc
     2641 cgtactctcc ttcagcgtcc ggttggtctg acgaacggcc atcccgacga cgataccggt
     2701 caggacatag aagacggccg caacaggcgt actgagaaag ccgtcgggaa tgtgcatcag
     2761 gatgggaatt gcgggaatct gcatcgcgtt ctccagactg gaccctgtca aacggcacca
     2821 cggcggttcc ggcgaccgtt cgtagttgcg actactcgca agtacggatg atggtaggcc
     2881 gctggatcat ccgcgtcaag tgcgggtgaa ggatcaagca gccttccggc agggacgagt
     2941 cggtgcctcg ctcctgccgg aaggctgcgg ttcggggaat tggcttgcgt atctcagcca
     3001 gaaatggctc tcgcgttggc ggacatctgt tctatggtag agagccacgt ccgcgcgtgt
     3061 tcgtgacaag ggagatcgac gcatgatcgc gaaaccaatg ttgacccgtg agtcgctgga
     3121 gcacttcggc aacctgccgg aggcctacca gcatgagtgg cgagtggcga cgccgctgga
     3181 gccgatcgtc gtcccggaag gcgtcttcaa gtggtaccac gttcaccgcg acggagtaga
     3241 ggtcgatgag agcctcgatc gagcggcgcg ggagctcatc tgcgacgggg cgatgacctg
     3301 gaaccttgaa tacgggctga acttcgcgct gctgcatcag agccacgagc gggcctttct
     3361 gatcatcggc gtctggcgtg gacatcagga gctgtggtcg cgggcgtacg agatcagcct
     3421 gctggacgac gacagcttcc ggcggatcga ggcaaccgag gagttctggc cgacggcttg
     3481 tgtctgggag atgggcgtca tccagcacga gcggatggcg tggcatcgct atctgttcac
     3541 cgagcacacc gacgccgaca agctggcctg gctggacgac cgctacgccg ggatggtctg
     3601 acaccggcgc gacattccct gaaatggagc tactgagtgg cgcgattcga tcccggccgg
     3661 ccggccggag atgagcacct cccgtactgg gggcagtaca tcgagctggt ccccgacgga
     3721 gatatcgccg acgtgcttga gtcgcagatc agcacgacag tggatagcct gcgggcgttg
     3781 acgccggtgc aggccggccg gcgaccggcg ccgggcgagt ggaacgcgct ggagatcgtc
     3841 ggccatctgg cggataccga acgcgtgctg gcgtaccgcg tgctgcggat cgcgcgcgcc
     3901 gacccggtcc tctgggaatc gattgagttc gagccctatg tggaacacgg cgaattcgac
     3961 gggcgcgagc tatggacgct catcgacgag tttgtggcga cacgcgccgc gacggtcgca
     4021 atgctgcggg ggctcggcga ggcagcctgg caacggcgga tgccggagga gtggtcgtgc
     4081 cgcagtgttc ggtcactcgc gtacgtgata gcagggcacg agctgcacca tctggctgac
     4141 atacgcggaa aggagcttgc ggcgcaagct ggagtacggc agatctgact cactcgcgat
     4201 gcagcgagaa cggaggacgc acaacgatgt cgtctacgag taactatgga ctcgacaacg
     4261 gatgggacaa ggcccgacaa cggctgacag gactggacgc gatggcggac gctggaacga
     4321 tccgccactt cgaagcgctg ggagtcgatg acggctggaa ctgcctcgaa gcgggagccg
     4381 gcggcggctc gatgaccgcc tggctctgcg accgagtcgg tccggacggg tcggtcgtcg
     4441 cggtcgatat caacacccgc ttcgtcgagg atctcgaccg cccgaacctg gaggttcgcc
     4501 agctcaacgt cgtcgacgag gccttaccgg ccggccagtt cgatctggtc cacacccggg
     4561 cggtgctggc ccatctgccc aaccgagagc cggcgatcga caacctgacg aacgcgctgg
     4621 cgccgaacgg ctggctgctg gccgaggaaa tggacttcct ctcggtcgcg ccggtgccgg
     4681 gcgtcgatcc ggcgcaggca gaccgcttca tgaagctcgt cgatgcccat caccaggcgc
     4741 tggcaggcaa gatggacccg ttctttggac gccgtgtgac ggacgcactc tgccagaccg
     4801 gcctggatga catcggctcg gagggtcgga cgacgatcgt gcggggcggg tcgcccggcg
     4861 cgacggcttg ggcgctcacg ttcgagcaac tctcggagac gatgctggag cacggctggg
     4921 tccgcgagac ggaactggct gaagtctatg atgcgctcga agatccctcg ttcgcgttcc
     4981 agtcgatggt gttcatggcc gcgtgggggc ggaaacgggg gtagaactgg ctccaattcc
     5041 aaatccaatc cggtaggggc gatttcgcga aatcgcccct accggattgg atttgttggg
     5101 tcagcagatc aaactgcgtc gtgtaacgca gccatccggg cgatgtcgtc gaggttggtc
     5161 cagtccatgt cgcgctggcc gtcctcgatc tcgtggatga cctggaggac agcggcgttg
     5221 acgggggtgg ggatcgagtt ggcagctccc ttctcgacga tgacggcgga ttgcatatcg
     5281 acttcggtct tgcgcttctt gatcttgagg tcgcgccaga tgcccatgtg ctgcttgatc
     5341 gaggctcgca tggcatcggc gagttcgtcg agggcggcgt tcgatcgctg ctcgaagtcg
     5401 tcaccggggg cgaaggcgtc aggatcgaat tcgccgatcc attcgaggtg atcggcctgc
     5461 gatttcgcga cgaggtaggc ctcggccgag gctgcctggc agatgcgccg gccgagcgga
     5521 ttgtcgatga cggtcgggac cggagcgtcg acgcaggaga cggcgaaagc catcgcgcca
     5581 tagacgagct tgccccagag gtagccccag atgttggtcg tcagttcgac cggcatgaca
     5641 tgcgagagcg ccgtttcgag ggcgcgcagc cgggaagtga tcgtgccgtc cagctcgccg
     5701 gggtggatcg tctgctcgtt gccgagctgg atcaggcccg gttccatgta gtcagcgccg
     5761 aagtggacga acgctccgac cgtccgttcg cggccgacgt ggcgggcgat gatctcctcg
     5821 ttgaggccgt tctggagcga gagaatgtag ccgtccggcg cgagaagcgg cccgacctgt
     5881 tgcatggccg gctcggtgaa atggcccttc acgcagagaa aggtgacgcc gagcggtccg
     5941 cgtaagtcgt tcgcgaggac ggccttgacc gggaaggtgc ggtcgccgcg caggccggtg
     6001 atgcgtagcc cacgctcatt catgatctgg acgtgctccg gaacgacatc gacgagcgtg
     6061 acgtcatagc cggcgtcggt cagaaacgcg ccgaccgttc cgccgatcgc cccggcgccg
     6121 acgatcgtga tgggtgcttc tcgaaggtca gtcgcttcca tttatcatgc tccctcgtga
     6181 cattcggtcg gggcagtgta ctctgatcgt gacatatgag tatcagggcg agcgacgagg
     6241 agggcctgtg cgctacggtc tgcatcacgt cagcattccg gtccgtcccg gacagcttga
     6301 ggccgggcgg gaattctatg gccaggcgct ggggctggag gagattccgg tgccggccga
     6361 gttcgaggaa gggcgggtcg tctggttcag tctcggtgac cgggagctcc atctctttaa
     6421 ggaagaggac gccaaccagc cgcgcagcga acgacacctg gcgctggagg tcgatgatct
     6481 ggcggcggcc agagcgcgct ttgcggagca tgggatcgcc gtcgaggaaa ccgacccgat
     6541 ccataatcgg ccccggctct acatccgcga tccgttcggc aacaagctcg aaatcaccga
     6601 gctgcaggga ccctatcggg aatagaccgc tggtagccag cgttacataa agtacgggcg
     6661 atttgtagat gtcaggtgac gggtgtcagg tgttagggaa gtagtccgtt cggattcggc
     6721 aggcacgttg tcttcacatc aatgaccgtt ctgacacctg acacccgtca cctgacgcct
     6781 ccaacaaagc ggtggcacgg aatcggcggg acgaaggctg acaacttcgc tgtgtgggtg
     6841 gcagcgaaga ccttctagtt cgctcgacgt aacgaaatgt atcgacagtg attccggtgt
     6901 agagacggat gcgcgcggac ttccgtcagg agtctggaca gatcggacga acgggtgtac
     6961 gttctaggta ttcgttcaca gccggagccg cggctgtggc ataccgttcg gccgggacga
     7021 cagttcgcct ctttacaaat ccgcaacatg gtctattcta gagagtgagt ttgtgaagaa
     7081 tcctacaaac tcgcctaatt acttgacata ttgtgagatt gctggtacac tacccaagtt
     7141 tcggcgcaag cacgcccaac caagcgtcag tgttgagacc agccggagat aacaggaacg
     7201 aaggagtctc atgaccgagc gagccattca ggacacctac gagctcgaga ggttggcggc
     7261 ggacgctgaa gtcggggatg taatcgatga gtcagcggag ctgatcgatc aggcaaccag
     7321 cgatgccgtc tatcgctact tccgcgatgt cggcggacac cggctcctct cgcacgaaga
     7381 agagatcgat ctgtcgagga aggtgcgcgc cggactcgag gtccgacgcc gccgtgaggc
     7441 cggcgaggac gtcgagaacc tccagcagac gctggaactg gcgcgtggcg cccgcgagaa
     7501 gctgatccgc cacaacctgc ggctggtggt ctcgatcgcc aagaagtatc gctccagcgg
     7561 cctgccgctg attgacctca tccaggaggg caacatcggc ctgatgacgg ccgtcgagcg
     7621 ctacgacccg gagctgggct accgcttctc gacctacgcg accttctgga tccgccaggc
     7681 gatcggccgg gcggttgcga acctgtcgcg gacgatccgc gtgccggtcc acatgcacga
     7741 cctgatctcg aaggtgcggc ggaccgagtc gcagctggag cagcagtacg gccggccggc
     7801 gaccgacgcc gaggtggcgg cccatctgga gatggaagtc gagcgggtcg agcaggcgcg
     7861 gtcagcgatc ccacggacgt cgtcgctgga caagccgatc ggtgaggacg gcgagtcgac
     7921 gatcggcgat ctgctgccgg atccggcctc ggaccaggtg gttgaggaag cggtgtcgag
     7981 cgcgatccgc gaccagatcc gcaagtccct cgacgcgctg accgagcggg agcggggcgt
     8041 gctgatgctg cgcttcggcc tcaacggcca gcagccgcag acgctggcgg agatcgccga
     8101 ccactacggc atcagccgcg agcgggtgcg ccagatcgag aaggaagcgc tcgccaagct
     8161 gcgaaagagc gacctgatcg agttggccaa cgcggcgtag cgcgccaacc gaacgagcac
     8221 gaagcgaacc cggccgggag tgatcccggc cgggtttgtc gtatgctacg ttgaacttgg
     8281 ttctgaacga cggacaagcg gacgacacaa aagattcggc acattggaga tgcgagagat
     8341 gatcatcgag acgcctccat acatgccgct cggcgttcga gaataccttg agctcgagga
     8401 agccagttcc gtacggcacg agtatgtggg cggaatcctg cacgcgctgg cgggagcgag
     8461 ccgccgtcac aatcagatca tcacaaacct gatcaccgat ctggggccga tcgccaatca
     8521 ggcgaactgc accgttcatc acgaaacggt caagcttcag gtcgccgacg acacgatcta
     8581 ctatccggat ttcatgatcg tctgccatct cgatgatact gatgcgttcg ttgtcgagcg
     8641 cccgtgcgtc gtcgtggagg tcgtatcatc atcatcacgt gtcattgatc gacgcgaaaa
     8701 ggtgatggca tatcgccgcg tcccaagcct gctggcgtat ctggtgatcg agcaggacga
     8761 gccgagcatt caacgacact ggcgcacgcc ggagggtgca tggctgcatg gaacgatcgc
     8821 tcgtgaaggc atcgttccga ttccgtgcct gggtacggaa ttatcttttg agcagatcta
     8881 ccgtcgaatc gacttctcca ccagctcggg gtgaacagcg attttatctg ccgataacga
     8941 atcaacggtc ggatcgatgt ccctctgcag cgtttcgctt gagggtctgt cgtcgcttac
     9001 agtgcttttc attccaaacc gtccgcgcag gcgatgggca accagcgacg ccagcgcaag
     9061 cggtgccgtc gtgacgaggc cgacgatgag cggcagccgg tggtcggtgg cgtagagtcg
     9121 accactggcg atcggggcgg tcgtgtcgcc gatgccgacg acgaactcgc agaggccgta
     9181 ggcgcgtccc cggacgcgct ccggggtgac ttcggcgacg gcggaggcca tcagcgacca
     9241 gatcggcgag aagcacatcc gcagcatgaa ggcgaaggcg acgatcggca gggccgtgaa
     9301 ggagagcatc agcgccattg agagggcggc gatcgagagg atggccgcca tccccatgaa
     9361 cgggctggtc agcggcttcc agtggctgac gagcaggctc atgccgaagg cgagaacggc
     9421 gccgatcgag gcgaggacac ctatcgttga gaggctgacg ccacggtcgt cgttcatgaa
     9481 gttgggcatc agcgcgaagc cgatgacgac cagcagcggg acgagcaggt ggaagccgag
     9541 gatgacgtgg acgccggtcg agcgcagcag atcgcggtag ccgatgggct gggcggcaga
     9601 cccggtctct gccggctcct ggctggggcg gatattgtcg atacgggttg cgacgacgat
     9661 accggccgcg aagagggcag cgccgacgag atagacggtc cggaagccag aggtttcggc
     9721 cagccagccg ccgatggccg gcgcgacggt catcgagatc agccacgaga gggtgaagat
     9781 gtaggtcatc gtccgggtac ggtcttccgg tttcgaggcg gcagcgacgt aggcggagac
     9841 ggccggaatc gcgacgccgg acatgtccat caggacggcg ccggccagcg cgtgccacca
     9901 ttgggtcgcc agtgcgaggc tgatcgcgcc gaagatcggg acggccatca tcgcgatgat
     9961 gacgggcttg agcgggaagc ggtcggccag gtggccggcc ggaatggcga ggatcgtgcg
    10021 gacgacggca ccgccgccga ggacgaggcc aatctgcgtc ggcgtcgcgc cgaggctctc
    10081 gatgaagagg gtccagaggg cggcctgaat cgtgaagcct gcgccccaga tgagatggac
    10141 ccaaaagaga atgccgttgt tgcggcccag gccaaggtct ggctggagcc agggccagcg
    10201 cggagtggat ggcccgccgg tcgattggtg ctgatcgtgc acggaaagat cccaacggtg
    10261 aactgcacgc gtggcgcgtg tcagcaggcg attcggacag tcaacaggga ttggcgcgcc
    10321 gctgtcgtca gcggcgtgcg gtcacgctcg gtgttgcgct cgctcgcgcg aggtgatggc
    10381 tagggtttcg ccaccggttc cacacgtgcg tcccttctga accgaatgcc tgcccggtgg
    10441 atgcgtccac ggcgggggtc gcaaccgtac cagacgtggg attggagcgt ccagtccccc
    10501 ctgtcgaaca cgtgatcgtc gcgcgatcag gcgagccagc gaccggcgcg catgacgtcg
    10561 cgagcgggga gttcggcggg gctgcgccag gcctggatac gctcaggggt gaggcggaag
    10621 aagacgtact cctgggtttg ctcacgggcg tcgaagccga cggcctgcgc gtgctcgtcg
    10681 gcgaagctgc cggcatcgct ggcgaggatc tggctgagtg tgccctcgat gatgacaacg
    10741 tcgagcggcg agccgagtgc gatccgtgcc cagccggcgc gacgcaggtt acgggccgtc
    10801 tggctggagc ggggcgtggc catcgtcacc agttcgccgt cccaggagaa cgagagcggg
    10861 atgatgtagg cgtcgccgct gccgttggcc gacgggaccc aggcgtcgcg ctcgctgcgg
    10921 agcagggtgc ggacgtcctg aatgcgctgc cgggtgtcgc gaacgtcgct catgcgcacg
    10981 cctgtcgttc ggggaagtgc tcggggaaac gggcgtacgt aaagatcgat gcggcctcga
    11041 cgtggataaa ggcgccgtca ccccagtcgt tgacgctgga agagccgaac gaacgatgca
    11101 gggcttcgag cgtcgcgaag gttgggtcgt cgctggtgag gagcgacgcc tgtccgtgga
    11161 cgatgacggc caggtcgttg ccttgatagt acgtcaggct gacggccggg cggcgctgga
    11221 tgtggcgcaa tcgggcggcg gtcgtcacgg ttgggatata gaatgcgccg cgatagaaga
    11281 gcgaaccgat cggagcgacg cgcggttcgc cggcagcggt gacggtcgcg aaggcgacgg
    11341 tctggatgcc ctggagatag gtcacgagct gacgcgccga gagcgacttc tctggcatct
    11401 cgaacgagct gcgcaggaag gctccggcgc gcttgatact gtcgtcgagc aacgtgctca
    11461 gtcgttcgag gtcttcgggc gtctcctgca cgaactctcc ttccgaggat gaatcatcga
    11521 tggccctcga acggtagcat gggcagcgtg ggatgggcat ccgaagaacg tcgtatcaat
    11581 acagatccag ctcgccacgg atgcgaacga ggcgttcgag ccaggcgaag gcgaggatgc
    11641 cggccagcgg gagggcgatg gcgaagccgg ccagcgggat gaggtcggac tggatctggg
    11701 tcaggctggc gtggtggagc gaggcgtcgg cgatggcctg gatgccgtag ccgaagggga
    11761 ggacgcgggc cagctggcgc atcccggtcg gcagttcgct gacggggaag tagaggccgc
    11821 cgagcaggac gacgaagggc cagatcaggt tcgagaaggc gttggcgcgc ttaaggatca
    11881 gaccgatacc ggcgaagatg agccccagcc cccagagcgc ggccatgaag agcaacagcg
    11941 agacggccag cgccggcagg ttgggatcaa gtgtgactcc gaaagccagc cagccgaaca
    12001 ggagcgaggt cgtcagcatc gagatggtct ggatcagtcc ggccagaatg aagccggaga
    12061 gccaggcggc gcggctgcac ggcgcgagga agagcgagac gagcgtgccc ttctcccgtt
    12121 cgcggtcgag gatctcgccg ttgtagtaga tcatcgtcat gatgaaggcc ccggcggcct
    12181 gagcgacgac gagatagccg agcaggtcgg acgaggtgcc gtagattagt gcgaagatcg
    12241 agatctgggt gatcgggatg atcatcatct ggatcagccc gccgcgcccc atgtgataga
    12301 gccactcgcc cgtagccgcc cgccaggagg cgatcaccga cgccagctcg tggcgcaggg
    12361 cgtcgagccg ggtgggccgg tcgcggtcga tagcgctaga agagatcgag ctggcccgcg
    12421 cgcttggcaa catcttcaac cctccgaatt ccgagaagtc cggtcagcac gaacaaactt
    12481 gatgtcagcg ccgctgcaag cagcgagttg ccgacatcgt ggagcgaggc agacgccagc
    12541 atgctggcgc gaaacgcgtc gacggcgtgc gcgagtggca tcgcattcga gatggtgtgc
    12601 aaccagtcgg gcagcgcgtc gcgcggaatc atgaagccgc ccatcagata gagcgggtgc
    12661 tggatgatgt tcgccatcag gttggcgcga cgactgagga tgaagagcga tgccagcagg
    12721 aaccccatcg caagcgaggc gaccagtagc gccagtgccg aggcgacgac ggcgaccagc
    12781 gatccgacgt tgaaacgcgc gccggtgatg acggcgagca ggacgatcgt cgcgaacgag
    12841 gggatcatgt agatgaagcc gcccaggccg tgaccgatga cgacggcgac gcggctggcc
    12901 ggcgtcagga agagggcggc gatcgtgcct tcccaacgtt cttcctcgat ggccgatccg
    12961 gtccaccaga ccgacgagta ccagcagatc atgccgacca ttccggccag caggaagcca
    13021 ctgatgttga caccgtcgat ggtcgtggcg ccggacgcct tc
//
LOCUS       DASUGJ010000520         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_916998, whole genome shotgun sequence.
ACCESSION   DASUGJ010000520 DASUGJ010000000
VERSION     DASUGJ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916998"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFV93_11760"
     CDS             complement(<1..399)
                     /locus_tag="VFV93_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166065.1"
                     /translation="MARGDNPTLAVEKAAVGRLGTGIALIAVFWTFSWLQLRPVSDYY
                     FFPLWLGYILTVDGLLALRTGTSPVQRCRWRVVVLFVVSVPLWWLFELFNQVVGNWRY
                     HAPVTYTTLEYALLASLSFSTVVPAVLTTTE"
     gene            507..1205
                     /gene="rpe"
                     /locus_tag="VFV93_11765"
     CDS             507..1205
                     /gene="rpe"
                     /locus_tag="VFV93_11765"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX5166066.1"
                     /translation="MIERRSDGALISPSVLNSDLTRLADSLRLLEQAGADYVHLDVMD
                     GTFVPNISIGIPVVASVQAETTLPLDVHLMIDAPERYIAAFVEAGADILTIQVESTRH
                     PHRALQQIREQGIRAGLAVNPGTPIEHAIELLELCDLVLIMSVNPGFGGQQFIPTTLR
                     RLRRLREAIETGGHDVVIEVDGGVSTRNAAEITAAGADMLVSGTALFGAPEGLVTAVR
                     ALRAASAETPGRSE"
     gene            complement(1240..2799)
                     /locus_tag="VFV93_11770"
     CDS             complement(1240..2799)
                     /locus_tag="VFV93_11770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166067.1"
                     /translation="MKPDSLILESPELTDRQQRQILLPVLTVVFLAALDLTVVAPILP
                     SVLNDLQINPVDADRYSWIVLSYLVAYTVTVPITGRISDFAGRIQVFTAAMLLFLVGS
                     VVVATSGSLAMMIAGRTLQGLGGGAMLPVSMALVADVVPRQRRAAALGMVAAVDTFGW
                     VLGPVWGAAVDGVFGSWRAIFWLNLPLGAAAAGLLYVTNARVVRRPGGKRPGLLSALA
                     GTVGLVAFCLALSSGSEGSLSSKQGAAKLGGSTNPLADYRWPILAVALVAFVAFVILE
                     RRAATPILPKSLIRSRVFQVAGGANVLVGAALITAMVNAPLAVALLVDDDDVARDSAL
                     LLGSLTIAMTVGAVVGGRSVDRLGPRRTAVAGLAVATLGFATMHWWPNEFHLGIMSLT
                     IAVAGLGLGFVIAPIGEVAIRAARTEDYGAASGLVLLARLLGMTVGLSAITAYGLGRL
                     DQKVDQIPPISPNPGESTTSYFTRQQDYFNSQVIPLTLDVIRETFVIAGLVCIIAVIV
                     VSRLRAASDES"
BASE COUNT          588 a          942 c          914 g          417 t
ORIGIN      
        1 ctcggtcgtc gtcaggacgg ccgggacgac ggtcgagaac gagaggctcg ccagcaacgc
       61 atattccaac gtcgtgtatg tcaccggtgc gtggtaacgc caattgccga caacctggtt
      121 gaacagctcg aacagccacc agagcggcac cgaaacgacg aagagaacga cgacccgcca
      181 gcgacagcgc tggaccggcg aagtcccggt tcgaagagca agcagtccgt cgacagtcag
      241 gatatagccg agccacagcg ggaagaagta atagtcactg accggacgca actgcagcca
      301 cgagaacgtc cagaagacgg cgatcagcgc gatcccggta ccgaggcggc caactgccgc
      361 cttctccact gcaagcgtcg gattatctcc acgcgccatc gccatcatgc tgggagtata
      421 tcgatgccag cggtgcgcct ggaattcggc cattcggcaa gatgatgacc ttgcatgttc
      481 gcgtgaaata ggttgcaatc cagcccatga tcgagcgacg gagcgacggc gcgctgatat
      541 cgccatcagt cctcaacagc gacctgaccc ggctggccga ttcgctgcgg ctgctggagc
      601 aggccggcgc cgactatgtc cacctcgacg tgatggacgg caccttcgtg ccaaacatct
      661 cgatcggcat cccggttgtt gcgtcggtcc aggccgaaac cacgctcccg ctcgatgttc
      721 acctgatgat cgacgctccc gagcgctaca tcgccgcctt cgtcgaagcc ggcgccgaca
      781 tcctcacgat ccaggtcgag tcaacccgcc acccacaccg cgcgctccag cagatccgcg
      841 agcagggcat ccgggccgga ttggccgtca atcccggcac gccgatcgaa cacgcgattg
      901 agctgctgga gttgtgcgac cttgtcctca tcatgagtgt caacccggga ttcggcggcc
      961 agcagttcat tccaacgacg ctgagacgcc tgcgccggct acgcgaggcg atcgagactg
     1021 gtggtcacga tgtcgtgatc gaggtcgacg gcggcgtctc aacgcgcaac gcggccgaga
     1081 ttacggcagc cggcgcggac atgctggtgt ccggcacagc cctgttcggc gcgccggagg
     1141 gactcgtcac ggccgtccgt gccctcaggg cggcatcggc cgaaacgcct ggaaggtcgg
     1201 agtaactccc cctacatggg agaggggcga tcatcacgtt caagactcgt ccgatgcggc
     1261 ccgtagccgc gagacgacga tgacggcgat gatgcaaacc agcccggcga tgacgaaggt
     1321 ctcgcggatc acgtcgagcg tgagcgggat gacctgcgaa ttgaagtagt cctgctggcg
     1381 ggtgaagtag ctggtcgtcg attcgccggg attcggcgaa atcggcggga tctggtcgac
     1441 tttctggtcc agcctgccca atccgtaggc cgtgatcgcc gacaggccga ccgtcatccc
     1501 aagcagacgg gccaggagca ccaatcctga cgccgcgccg tagtcctctg ttcgcgctgc
     1561 ccggatggcg acctcgccaa tcggtgcgat gacgaatccg agtcccagac cggcaacggc
     1621 gatcgtcagc gacatgatgc cgaggtgaaa ctcgttcggc caccaatgca tcgtcgcgaa
     1681 ccccagcgtg gcaacggcga gaccggcgac tgccgtccgg cgtgggccga ggcgatcgac
     1741 ggagcggcca ccgacgacgg cgccgaccgt catcgcaatt gtcaggctgc cgagcagtag
     1801 ggccgaatct cgcgcgacat cgtcatcgtc gaccagtaac gcgaccgcca gcggcgcgtt
     1861 gaccatcgcc gtaatcaatg ccgcgccgac cagcacgttc gcgccgcctg ccacctgaaa
     1921 gacgcgcgag cggatgagcg acttcggcag gatcggcgtc gccgcgcgcc gttcgaggat
     1981 gacgaaggcg acgaacgcga ccagtgcaac cgccagaatc ggccagcggt aatcggccag
     2041 cggatttgtg gagccaccca gcttggccgc gccctgcttg gaactcaacg agccctcgct
     2101 accagacgac agcgcgaggc agaacgccac cagcccaact gtcccagcca gtgccgagag
     2161 gaggccaggc cgtttcccac ccggccggcg aacaacgcgg gcgttcgtga catagagcaa
     2221 gccggcggcc gccgcaccga gcggaaggtt gagccagaag atcgcccgcc aggagccgaa
     2281 tacaccatcg acagccgcgc cccagaccgg tcccaacacc cagccaaacg tgtcgacggc
     2341 ggcgaccatc cccaacgcgg cggcacggcg ctgacgcggg acgacatcgg ccaccagcgc
     2401 catcgagacg ggcagcatcg cgccgccacc cagcccctgt agcgtccgcc cggcaatcat
     2461 catcgccagg ctgccggagg tggcgacgac aaccgagccg accaggaaga gcagcatcgc
     2521 agcggtgaag acctggattc gaccggcaaa gtcagagatc cgacctgtga tcggaacggt
     2581 gacggtgtag gcgaccaggt aggagaggac gatccatgaa tagcggtcgg cgtcgaccgg
     2641 attgatctgc aggtcgttga ggacagatgg caggatcggc gcgacgaccg tcaggtcgag
     2701 cgccgccagg aagacgaccg tcaggaccgg cagcaggatc tggcgctgct gccggtcggt
     2761 cagctccggc gattccagga tcagggagtc cggcttcaca cttccggcgc ctcgatctcg
     2821 acgtcctcgt tgtggtctgt gagatcgaga ttccaggttg c
//
LOCUS       DASUGJ010000521         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_361077, whole genome shotgun sequence.
ACCESSION   DASUGJ010000521 DASUGJ010000000
VERSION     DASUGJ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361077"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFV93_11775"
     CDS             complement(<1..921)
                     /locus_tag="VFV93_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5166068.1"
                     /translation="MSGLAGLPVSLRLYILTIAVLGPAICLVSALVGGVWTNTTELLI
                     ALLLVAMAAIANNYNFNLSHQTSINVSDAAYVAMIIVLPPGAPGLLALVAIAIGQFLR
                     RPDPVEVPFNIGQGALHVTTGALAYWAMLQVAPASPDWLHDLTIAGAVGVAALTMHLV
                     NCALVSVAVGLQLQSSPFRIWARNLGQDLPVTAVLAALGGVAAIIALAEPLALPLLAM
                     PVILVRYAAKQLVQLRKDTMAAMASMVDIIELRDPYTAGHSRRVAEMARALALRLGLT
                     AEEADLLEAAGRVHDIGKVAVDPEVLTKPGK"
     gene            complement(991..1536)
                     /locus_tag="VFV93_11780"
     CDS             complement(991..1536)
                     /locus_tag="VFV93_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166069.1"
                     /translation="MVTSEKGHNSKRRAWRLPVRARLMLVLTLATLAFVTMAPAASAA
                     RAWCRTDPVVVIDGTFADVFVSGPLLAPLTVTGPTQLIITVPEGVKTQMILSDLGFLH
                     GYDYQFKQSSELRKTRKGVEVRVEVYVPASDDSMPVKVEFSPRLLGVLGLLGLNPSTV
                     SAEGTANEWITLNAFLKYGLL"
     gene            1834..>2502
                     /locus_tag="VFV93_11785"
     CDS             1834..>2502
                     /locus_tag="VFV93_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5166070.1"
                     /translation="MSDLVFRSAGELARMVRQRDVSAVEVVEAHLTRIDRLNGALNAI
                     VTLDADGALAQARQIDAGQGDGALRGVPITLKDCHETAGMRTTSGHPPLANYVPAADG
                     TIAGRLRAAGAIILGKTNVSELLADAQSDNPVFGRSNNPWNLDRTPGGSSGGAAAAVA
                     AGLAPLDIGSDIGGSIRMPAHCCGIAGIKPTERRVSNHGHIPDLPGQPRGARYMNSIG
                     PLARD"
BASE COUNT          485 a          852 c          792 g          373 t
ORIGIN      
        1 cttgccgggc ttggtcagca cctcgggatc gacggcgacc ttgccgatgt cgtggacccg
       61 gccggccgct tcgagcaggt cggcctcctc ggccgtcagc cccagccgca gcgccagcgc
      121 ccgcgccatc tccgccaccc ggcgcgagtg gccggccgtg tacggatcgc gcagttcgat
      181 gatgtcgacc atcgaggcca tggcggccat cgtgtccttt cgaagctgga caagctgctt
      241 ggccgcgtag cggacgagga tgaccggcat ggccagcagc ggcagcgcca gcggctcagc
      301 cagcgcgatg atggccgcaa ccccaccgag cgccgccaga acggctgtga ccggcagatc
      361 ctgtccgagg ttcctcgccc agattcggaa ggggctcgat tgcagttgca acccaaccgc
      421 gaccgacacg agcgcgcagt tgaccaggtg catcgtcagc gccgcgaccc cgaccgctcc
      481 cgcgatcgtc aggtcatgca gccagtcggg gctggctggg gcgacctgga gcatggccca
      541 gtaggccagc gcgccagtcg tgacgtgcag cgcgccctgc ccgatgttga acggcacctc
      601 gaccggatcg ggacgacgca ggaactggcc gatggcgatc gccaccaacg ccagcagacc
      661 cggcgctccc ggcggcagga cgatgatcat cgcgacgtag gccgcgtcgg agacgttgat
      721 cgaggtctgg tggctcaggt tgaagttgta gttgttggcg atggccgcca tcgcgaccag
      781 cagcagcgcg atcagcagtt cggtcgtgtt ggtccaaacg ccgccaacca acgcggagac
      841 aagacagatc gccggcccga ggacggcgat cgtcagtatg tatagtcgta gggataccgg
      901 tagcccggcc agtcctgaca cgtctgagtc tccacgaccg cccccggagc aacttccaga
      961 ggggcggcct cgtcgtctac agggtcacgc tcacagcagc ccgtacttca ggaacgcgtt
     1021 cagcgtgatc cactcgttgg ccgttccttc ggccgaaaca gtcgacgggt tcagcccaag
     1081 caatccaagc acgccgagca gtcgcggcga gaactcgacc ttgaccggca tcgagtcgtc
     1141 gctggccgga acgtagactt caacccgcac ttcgacaccc ttccgcgtct tccgcagttc
     1201 gctcgactgc ttgaactggt agtcgtagcc gtgcaggaag cccaggtcgg acaggatcat
     1261 ctgcgtcttg actccctcag ggacagtgat gatcaactgg gtcggacccg tcaccgtcaa
     1321 tggcgccagc agcggcccgg acacgaacac gtccgcaaac gtgccgtcga tcacgacgac
     1381 cgggtccgtt cggcaccacg cgcgcgcggc cgaagccgcc ggagccatcg tcacgaacgc
     1441 aagcgtcgcg agtgtcagca ccagcatcag cctggctcgc accggcaggc gccatgcccg
     1501 tcgcttcgaa ttgtgcccct tctcgcttgt caccatctat acccccatag tcacgatatc
     1561 aacccgctga attgggcaga aaattcggaa aaccacgtct gtgatggtca gaatgtagca
     1621 accgggatag tcctggggca cgtgccagaa gtactattgg aatcaagtcc ttagcaggta
     1681 ccaaactgtt cacaactccc atgtttccgc gccggattca gaagacatgc gaaagaagac
     1741 gatgatgagg acgaggacct gacgacgaca acgccccccg gaagtactat ggaaaacgat
     1801 gcgacgccga tgcctgccga gaggagtcct ggtatgagcg atctggtgtt tcgaagtgcg
     1861 ggcgagctgg cgcggatggt ccgccagcgc gacgtgtcgg cagtcgaggt cgtcgaggca
     1921 cacctgaccc ggatcgaccg tctcaacggg gccctcaacg cgatcgtgac actcgacgcc
     1981 gacggcgcgc tagcgcaggc gcgacagatc gacgccggac aaggtgatgg cgcgctgcgc
     2041 ggcgtcccga tcacgctcaa ggattgccac gagacagccg gaatgcgcac aacatccggg
     2101 cacccgccgc tggcgaacta tgtcccggcc gcagacggca ccattgcagg gcgtctgcga
     2161 gcagccgggg ccatcatcct cggcaagacg aacgtctcgg agctgctggc cgacgcacaa
     2221 tccgacaacc ccgtcttcgg ccgcagcaac aacccgtgga acctcgaccg aacgccgggc
     2281 gggtcgagcg gcggcgcggc ggcggccgtc gcggccgggc tggcaccgct cgacatcggc
     2341 agcgacatcg gaggctcgat tcggatgccg gcccactgct gcggcatcgc cggcatcaag
     2401 ccgaccgagc ggcgcgtctc caaccacggc cacatccccg acctgcccgg ccagccacgc
     2461 ggcgcacgct acatgaacag catcggcccg ctggcgcgcg ac
//
LOCUS       DASUGJ010000522         6320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1333222, whole genome shotgun sequence.
ACCESSION   DASUGJ010000522 DASUGJ010000000
VERSION     DASUGJ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333222"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..399)
                     /locus_tag="VFV93_11790"
     CDS             complement(118..399)
                     /locus_tag="VFV93_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166071.1"
                     /translation="MSRHRAYREFTDDDLDYLYVVVPTDDGQTTELLTARTMSDRQFR
                     EWIVAKGKLHNIQILPTFGRLGMETRLALVNRLIRNGVHIHLAPRNPDA"
     gene            543..2051
                     /gene="pyk"
                     /locus_tag="VFV93_11795"
     CDS             543..2051
                     /gene="pyk"
                     /locus_tag="VFV93_11795"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5166072.1"
                     /translation="MSASTPTGVTSAQPAQRAWGSDANFRRNVKIVATLGPAVDDHES
                     LSSIIDAGVDMVRINCAHGTPESRSQMIQLARAIADERGQPLPVLMDLQGLKIRTGPL
                     ADNQPAMLAQGAKIRVYPQLVPTTAEQIGVSYPQLLQVIRPGQRILLADGLIELLVET
                     VHRDYAACSIGRGGPLAARQGVTLPSVVLQDAVLTENDRNDLAFAAEHDVEYVGLSFL
                     SSALDIERTRGAAMSLGWKPGIVAKIERPAALAQIESIAAAADVVMVARGDLGVQLPP
                     EQVPRAQKEIVAVCNRLGTPVITATQMLESMIMQPVPTRAETSDVANAVLDGTDAVML
                     SAETATGRYPASAVEMMDRIIREVERFGPIRPQDQREVAPATDSDRMIVDALGRAARA
                     LSDVAPINYIIVFTLSGSSARLVAKGRPRVPIIAMTTDPFVARQLSLVWGVHALVSPL
                     IDNVDDLFKEAATQVIDAGLAHEGAEALFVGSLPIYRVSGRTNLLHVRKLEH"
     gene            2143..2361
                     /locus_tag="VFV93_11800"
     CDS             2143..2361
                     /locus_tag="VFV93_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166073.1"
                     /translation="MHDTSGNAYDSPIDNQTYNLLQILTSKLEAVEAYNIYEEDMDGE
                     SAALMDRIAADDRRHVAELAKTLGLSRS"
     gene            2490..4103
                     /locus_tag="VFV93_11805"
     CDS             2490..4103
                     /locus_tag="VFV93_11805"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX5166074.1"
                     /translation="MEMIEVRDLDGPNLFALHPVIKLEVRLDPDDTVSPDARVAARSP
                     FGADLPAEPIPALREAIAALHRRVGLPVPEIGVRALDTPGHEVVFFDWSYRETALEIG
                     RQAVAAVSARLANDSYLTLERLLKADRENDDHPLWIRDEQRRLPSVGITGTNGKTTTT
                     RMVAHIVRQAGYHAGWTSTSGVYIDGEQVVEGDYTGPSGARRVLLDPTVDYAILETAR
                     GGLLLRGLACESNDVGVFLNVSPDHLDMQGVETVETLAEVKSVVVRVTRPDGLVVLNA
                     DDPLVLAQREHVRAPVLLTSQNPASEPVARHVADGGQAIVRDGDMIWRYAGPVRQPLI
                     ALDDVPATFGGAATHMVENALAAIGAAIGLGFTDDQIVEGLQRFRSDIRSNAGRLNVF
                     RLDGRVIVVDYAHNEQGLEMLLAFARRIAAQGARVVAIVGTAGDRQDVQLRGLGTIGA
                     EQADRVYLKETGRYLRGREPGEVTALMQDGVACSQHPERLAGCFDGEHAAFLAAISDT
                     GPGDAIAIMCLEEQLTVLRELRDRGAEEW"
     gene            4156..4695
                     /locus_tag="VFV93_11810"
     CDS             4156..4695
                     /locus_tag="VFV93_11810"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5166075.1"
                     /translation="MTINAGSSYDTSVPPINDDTIDHHCFGCGNQNPMGLRLRFRPLS
                     DESVWATFTPTRDHEGYMGMTHGGIISTVLDEAMSWAVTHAGDLGVTARMSVTFRKPI
                     RLGQQIRVEATVVGRKARAIDTHAVVYDAATEQPLAEADGRFMRVTAEQAAAWRSAYG
                     DQIEESAFGAAARRNAGLA"
     gene            4791..5594
                     /locus_tag="VFV93_11815"
     CDS             4791..5594
                     /locus_tag="VFV93_11815"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX5166076.1"
                     /translation="MSDYTRGPLLAIGGAEDKFRDRAILRRFTELSGGDDARIAIIPT
                     ASAIDDAGQRYKAIFLELGATTAEVLFVGSRDDANSDELVGLIEQSTGIFMTGGNQIR
                     VASILGGTRVAEALVRRNREGVPVGGTSAGASVMSTVMVAGGKNGVSPRPNGAAMSPG
                     LGLIDSVIIDQHFRERDRIGRLVTMVSYNPGLLGLGVDEDTAALIWPHGTLDVIGKGS
                     VLIVDGANMRSDVYAGRGKHPLTIANVVVHFVAEGGSYDLTQRKVLCLR"
     gene            5708..>6320
                     /locus_tag="VFV93_11820"
     CDS             5708..>6320
                     /locus_tag="VFV93_11820"
                     /inference="COORDINATES: protein motif:HMM:NF040140.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166077.1"
                     /translation="MPLRILDSKIYRGPNIWARVPVILLKVDIEELEDRPTNTIEGFN
                     DELIGLIPSLYDHYCSLGRPGGFLERLREGTWMGHVLEHIALELQTLAGSEGTRGLTR
                     STDDRGVYNVVYQYAQHDLGMAAGELGVRLLNSLIYKDDAEFDFSRELERLIRLAERL
                     AYGPSTKALVDEAERRGVPVIRLDPRRSLVQLGYGIYQKRIWAT"
BASE COUNT         1113 a         2007 c         2133 g         1067 t
ORIGIN      
        1 gtcgatgcgc tcgaccgcgt tctggcccgg caggcagagg cgacagcagt tccctccatc
       61 gagccggcgc atgcactgac cgcgcggacg atggctgact gaggctcgct cggcccgtca
      121 ggcatccggg ttacgcggcg cgaggtggat gtggaccccg ttacggatca ggcggttgac
      181 gagtgccaac cgcgtctcca tccccagccg gccaaacgtc ggcaggatct ggatgttgtg
      241 cagcttgcct ttagcgacga tccactcgcg aaactgacgg tcgctcatcg tgcgggcggt
      301 cagcaattcg gtcgtctggc cgtcgtcggt cgggacgacg acgtagaggt agtcgaggtc
      361 gtcgtcggtg aattcgcggt aggctcggtg gcgagacacg atcatccttc tggttctcca
      421 gcgtgacagg ttcaggaatg gcaggcaacc gccgtgcgat agtatagcgg tgccagcccg
      481 gagcgggcag ccgcgcggcg cgtggcgacc cgctgccagc ccagacttgg aggagcctcc
      541 tgatgtccgc atcaacaccg acaggcgtca catccgctca gccggcacag cgcgcctggg
      601 gcagcgacgc taacttccgc cgcaacgtca agatcgtcgc gacgctcgga ccggccgtcg
      661 acgaccacga gagtctctcc agcatcatcg acgccggcgt cgatatggtc cggatcaact
      721 gcgcacacgg gacaccggag agccggtcgc agatgatcca gctcgcgcgg gcaatcgccg
      781 acgagcgcgg acagccgctg ccggtgctga tggacttgca ggggctgaag atcagaaccg
      841 gaccgctggc ggacaaccag ccggccatgc tggcacaggg cgcgaagatc cgcgtctacc
      901 cgcagctggt cccgacgacg gccgagcaga tcggcgtcag ctatccccag ttgctgcagg
      961 tcatccggcc gggacagcgt atcctgctgg ccgatgggct gatcgagttg ttagttgaga
     1021 cggtccaccg tgactacgcc gcctgctcga ttggccgggg cggcccgctg gcggcccggc
     1081 agggcgtgac gctgcccagt gtcgtcctcc aggacgccgt cctgaccgag aatgaccgca
     1141 acgacctcgc ctttgcggcc gagcacgacg tcgagtacgt cggcctgtcg tttctcagca
     1201 gtgcgctgga catcgagcgc acgcggggcg cggccatgtc gctgggctgg aagccgggta
     1261 tcgtcgccaa gatcgagcgc ccagccgcgc tggcgcagat tgagtcgatc gcggccgccg
     1321 cggacgtcgt gatggtcgcg cggggcgatc ttggtgtcca gctcccaccg gaacaggtgc
     1381 cccgggcgca gaaggagatc gtcgccgtct gcaaccggct cgggacgccg gtcatcacgg
     1441 ccacgcagat gctcgagtca atgatcatgc agccggtgcc gaccagggcc gagacgagcg
     1501 acgtcgccaa cgccgtcctc gacggaaccg acgcggtcat gctgagtgct gagacggcga
     1561 cggggcgcta tccggcatcg gccgtcgaga tgatggaccg catcatccgc gaggtcgagc
     1621 gcttcgggcc gatccggcca caggaccagc gtgaggttgc cccggcgacc gattcggatc
     1681 ggatgatcgt cgacgcgctg ggtagagcgg cccgggcgct ctccgatgtc gcgccgatca
     1741 actacatcat cgtcttcaca ttgagcgggt cgagcgcgcg gctggtcgcc aagggccgcc
     1801 cgcgggtgcc gatcatcgcg atgacgaccg atcccttcgt cgcccggcag ctctcgctgg
     1861 tctggggtgt ccatgcgctc gtctcgccgc tgatcgacaa cgtcgacgat ctcttcaagg
     1921 aggcagcgac gcaggtgatc gacgccggat tggcccacga gggtgccgag gcactgttcg
     1981 tcgggagctt gccgatctac cgcgtcagcg gtcggacgaa cctgttgcac gtccgcaagc
     2041 tcgaacattg accgtgcgtg gcatatcccg tgcaaaacag acacaacggg cgacgagcgt
     2101 ctgcccgatg tgttcacaca gaacgggatt tggagggcca cgatgcacga cacgtccggc
     2161 aatgcatacg actccccgat cgacaaccag acctacaacc tgctgcagat cctgacgagc
     2221 aagctcgaag cggtcgaggc ctacaacatc tacgaggaag acatggacgg cgaatcggca
     2281 gcgctgatgg accgcatcgc cgccgacgat cgccgccacg tcgccgaact ggcgaagacg
     2341 cttggtctgt ctcgcagctg atcgcaagcg cgttacaatt ccgtcgcttc ggcgacaacc
     2401 aaccgcaacg ggtcggggtg ttgtgcagcc cccggccccg tttaatcgtt ggggcaatcg
     2461 gacgcggctg gcagcctggg aggcacgtca tggagatgat cgaggttcgc gatctggacg
     2521 gacccaacct gttcgcgctc catccggtca tcaagctcga agtgcgtctc gaccctgacg
     2581 acaccgtctc gccagatgcc cgcgttgctg cccgctctcc gttcggagca gatcttcccg
     2641 ccgagccgat tcctgccctc agagaggcga tcgcagcgct ccaccgtcgt gttggtcttc
     2701 cggttccgga gattggtgta cgcgcgctcg atacgccggg ccacgaggtc gttttcttcg
     2761 actggtcgta tcgcgaaacc gctctggaga ttggccgcca ggccgtcgct gccgtctcgg
     2821 cccggctcgc caacgattcg tatctgacgc ttgaacgtct gctgaaagct gaccgtgaga
     2881 acgacgacca cccactctgg attcgcgacg agcagcggcg actgccttct gtcggcatca
     2941 ccggcaccaa cggcaagacg acgacgacac ggatggtcgc ccacatcgtt cgccaggccg
     3001 gctaccacgc cggctggacg agcacgagtg gcgtctacat cgacggcgag caggtcgtcg
     3061 aaggtgacta caccggcccg tccggcgcca gacgtgtact gctggacccg accgtcgact
     3121 acgcaatcct tgagacggcg cgcggtggcc tgctgctgcg cgggctggcc tgcgagtcga
     3181 acgatgtcgg cgtctttctg aacgtcagcc ccgaccacct ggatatgcag ggtgtcgaga
     3241 cggtcgagac gctggccgag gtcaagagcg tcgtcgttcg cgtcaccagg ccggacgggc
     3301 tggtcgtgct gaacgctgac gatcccctgg tgctggcgca gcgcgagcat gtccgggcac
     3361 cggtcctgtt gactagtcag aatcccgcca gtgagcccgt tgcgcggcat gtcgcggacg
     3421 gtgggcaggc gatcgtccgt gacggcgaca tgatctggcg ctacgccggc cctgtacggc
     3481 agccattgat cgcactcgac gatgtcccag ccaccttcgg cggcgcggca acccatatgg
     3541 tcgagaacgc gctggccgcg atcggcgcag cgatcgggct gggtttcacg gacgaccaga
     3601 tcgtcgaggg cttgcagcgc ttccgctctg acatccgctc gaatgcgggc cggctgaatg
     3661 tcttccggct ggacggtcgt gtcatcgttg tggactacgc gcacaacgag caggggctcg
     3721 agatgcttct ggcgttcgca cgccggatcg cggcgcaagg cgcgcgggta gtcgccattg
     3781 ttgggacggc cggtgaccgg caggatgtgc agctacgcgg gctggggacg atcggggccg
     3841 agcaggccga tcgcgtctat ctgaaagaga ccggtcgcta cctgcgcggg cgtgaaccgg
     3901 gcgaagtgac ggcgctcatg caggatggtg ttgcgtgctc gcaacatcct gagcgactgg
     3961 ccggctgctt cgacggcgag cacgccgcct tcctggccgc catctccgat accggtcccg
     4021 gcgacgctat cgcgatcatg tgccttgagg agcagctgac ggtcctgcgc gagctgcgtg
     4081 accgtggcgc cgaggagtgg taagacgtcc tcagacgatg gcagcgacct gacgaatctc
     4141 agaaacagga cgtgaatgac gatcaacgct ggctccagct acgacacgag cgttccaccc
     4201 atcaacgacg acaccatcga ccatcattgc tttggctgtg gcaaccagaa cccgatgggg
     4261 ctacggttgc gcttccggcc actttcggac gagagcgtct gggcgacctt cacgccgacg
     4321 cgagaccacg agggctacat ggggatgacg cacgggggca tcatctcgac agtgctcgat
     4381 gaggcgatga gctgggccgt cacacacgca ggcgacctcg gcgtcacggc caggatgtcg
     4441 gtcacctttc gcaagccgat ccggctcggc cagcagatcc gcgtcgaggc aaccgtcgtt
     4501 gggcgcaaag cccgcgcgat cgacacgcac gccgtcgtct acgatgccgc gactgaacag
     4561 ccgttggcgg aggcggacgg gcgattcatg cgggtgacgg ccgagcaggc ggctgcctgg
     4621 cggtcggcct atggtgacca gatcgaggaa tcggccttcg gcgcggcggc gcggcgcaac
     4681 gccgggctcg catgatccag atcgagggag ctagcgttga ccgattcgag cgcgcttccg
     4741 tatagtcacc cgagcgcgaa tggcgcagac gttgcgggtg gatggatagt gtgagcgact
     4801 acacgcgagg accgttgctg gcgatcggcg gcgccgagga caaattccgc gaccgggcga
     4861 tcctgcgtcg cttcaccgag ctgtcaggcg gcgatgacgc gcgaatcgcg atcatcccga
     4921 cggcgtccgc gatcgacgat gccggccagc gctacaaggc gattttcctg gagcttggcg
     4981 cgacgacggc cgaagtgctc ttcgtcggta gccgcgacga cgccaacagc gacgagctgg
     5041 tcgggctgat cgagcagagc accggcatct tcatgacggg tggcaaccag atccgcgtgg
     5101 caagcatcct tggcggcacg cgggttgccg aagcgctcgt gcgccgcaac cgtgagggcg
     5161 ttccggtggg cggcaccagt gccggcgcat cggtcatgtc aaccgtgatg gtcgctggcg
     5221 ggaagaacgg cgtctcgccg cgtccgaatg gtgccgctat gtcgccaggg ctgggcctga
     5281 tcgactctgt catcatcgac cagcacttcc gcgagcgcga ccggatcggc cggctggtga
     5341 cgatggtcag ctacaatccg ggcctgctcg ggctcggagt cgatgaggac acagcggcgc
     5401 tgatctggcc gcatgggacg ctggatgtca ttggcaaggg gtcggtcctg atcgtcgacg
     5461 gcgccaacat gcgcagtgac gtttacgcgg ggcgcggcaa gcatccgctg acgatcgcca
     5521 acgtcgtcgt ccacttcgtt gccgagggtg gcagctacga cctcacgcaa cgcaaggtgc
     5581 tctgcctcag ataggctccg gtgagcgcaa caggcggccg ggcagatcgt cgcataaact
     5641 gggtcacaat cgatagattc gggtgttcgg gtgtctgtcg tgacactgat gggagtgggg
     5701 gccggcgatg ccactgagaa tcctggactc gaaaatctat cgaggcccga acatctgggc
     5761 cagagtgccg gttatcctcc tcaaggtcga tatcgaagag ttggaagatc ggcccacgaa
     5821 cacgatcgag ggcttcaacg acgagttgat cggcttgatc ccgtcgctct acgatcacta
     5881 ctgctcgctt ggccggcccg gtggattcct cgaacggctg cgcgagggta cctggatggg
     5941 ccacgtcctg gagcacatcg cgcttgagtt gcaaacgctt gccgggtcgg aaggaacacg
     6001 cggcctgacc cgctcgaccg acgatcgtgg cgtctacaac gtcgtctacc agtatgcgca
     6061 gcacgacctc gggatggcgg ccggtgagct tggcgttcgt ctgctcaaca gtctgatcta
     6121 caaagacgat gccgagttcg acttctcgcg cgagcttgag cgcctgatcc gcctggccga
     6181 acggctggcc tacgggccca gcacgaaggc actggtggac gaggccgagc ggcggggagt
     6241 gcccgtcatc cggctcgatc cgcgccgttc gctggttcag cttggctacg gcatctacca
     6301 gaagcgcatc tgggcgacgg
//
LOCUS       DASUGJ010000523         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2028701, whole genome shotgun sequence.
ACCESSION   DASUGJ010000523 DASUGJ010000000
VERSION     DASUGJ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028701"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFV93_11825"
     CDS             <1..414
                     /locus_tag="VFV93_11825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5166078.1"
                     /translation="GAVLVGPTADASARSMPHLLWRLARDLVHEPFSLWLIQGRDYLR
                     FGPRWQWQTARAMLADRIEDKLPRLAMPVLVVRGEHDPIAPQAWIERLANFAPAGEWT
                     VVPGAAHAVNYTAPEALATLIDRLVGRVGDTGRSR"
     gene            411..1142
                     /locus_tag="VFV93_11830"
     CDS             411..1142
                     /locus_tag="VFV93_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5166079.1"
                     /translation="MSDWRWAELDDARLHYRQLGSGKPVVLIHGLSASGRWWHRNVDA
                     LAAHFTVYVIDLIGFGRSRGGRRFVLAEAAALVAGWMDAIGLERANVVGHSMGGYVAA
                     QLAAEFPEKVDRLVLVDAAVPLPRQQRLHHLTHLGREMRYTPPRFLHLLATDALLAGP
                     RTIWAAANQLIAADLEPKLDRISAPVLLVWGEHDPLVPASVADRLAERIPNVRRVVIP
                     NCGHKPMLERPNEFNEAVIAFLAGQ"
     gene            1232..1876
                     /locus_tag="VFV93_11835"
     CDS             1232..1876
                     /locus_tag="VFV93_11835"
                     /inference="COORDINATES: protein motif:HMM:NF023096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HEX5166080.1"
                     /translation="MAQPDAVKTRKQGRIRPWIGEPPREVAPEVTGYRLDSHQRFDML
                     QGSIAGMVLIPFWFVFFSVLIGLVGGRYMGEFAITIANMLLGAVIALVVVPALHEAVH
                     GLAAMLCGVRPSYGIGPGFAYTTFPDPVGKRAYLAIGLAPLVVLSLIGFPLAAVWDSG
                     AGAVLFFMVVNAAGAVGDLWMSWRIALQPPHALFVDLADGFAVLIPDAPEPVAP"
     gene            complement(1941..>2372)
                     /locus_tag="VFV93_11840"
     CDS             complement(1941..>2372)
                     /locus_tag="VFV93_11840"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166081.1"
                     /translation="VAIEVSDDDDLASFMQKLFSQAPGVIATALEKVIAGDPGEPQDE
                     SQASYYGAIPDELRVIDWNSPAAHIYNQIRGLSAFVDPPGAFGTVDGVPARILKARLM
                     PDAGGDGHQPGSVIDRRADGVVVQCGDGAVQLLYHEPVGTS"
BASE COUNT          366 a          768 c          805 g          433 t
ORIGIN      
        1 ggggcggtcc tggtcggccc tactgccgac gcgagcgcgc gatcgatgcc gcacttgctc
       61 tggcggctgg cgcgcgacct ggtccacgag ccgttcagcc tctggctgat ccagggacga
      121 gattacctgc gtttcggccc gcgctggcag tggcagactg cgcgggcgat gctcgccgac
      181 cggatcgagg ataagctgcc acggctggcg atgccggtgc tcgtcgtgcg tggcgagcac
      241 gacccgatcg cgccacaggc gtggatcgag cggctggcga atttcgcgcc ggccggggaa
      301 tggacagttg ttcctggtgc ggcccatgcg gtgaactaca ccgcgccgga ggcgttggcc
      361 acattgattg acagactggt tggtcgggtc ggagatacgg gacggagcag atgagcgact
      421 ggcgctgggc cgagctggat gatgcgcggt tgcactaccg gcaactcggg agcggcaaac
      481 cggtcgtcct gatccatggc ctgtcggcat cgggtcgctg gtggcatcgc aacgtcgatg
      541 cgctggcggc acatttcacc gtctacgtga tcgacctgat cggcttcggc cgtagccggg
      601 gcggccgccg cttcgtgctg gctgaggcag ccgcgctggt ggccggctgg atggacgcga
      661 tcgggctgga gcgggccaat gtcgtcggac actcgatggg tggctacgtc gccgcgcaac
      721 tggcagccga gttccccgaa aaggtcgacc ggctcgtgct ggtcgacgcc gctgtcccgc
      781 tgccgcgcca gcagcgcctg caccacctga cccatctcgg acgtgagatg cgctacacgc
      841 cgccccgctt cctgcatctg ctggcaaccg acgcgctgct ggccgggccg cgcacgatct
      901 gggcagctgc caatcagttg atcgccgccg acctcgaacc gaagctggac cggatcagcg
      961 cgccggtcct tctggtctgg ggcgaacatg atccgctcgt cccagccagc gtcgccgatc
     1021 ggctggccga acgcatcccg aatgtccgac gggtcgtcat ccccaactgc ggccacaaac
     1081 caatgctgga gcggccaaac gagttcaacg aggccgtgat cgcctttctt gcgggccaat
     1141 agcgcttacc accccgtgca gcggccgatc ctggcgaact ggttgacgca tacttcatca
     1201 acgacacgga accgaacggc aaggaacgag cgtggcgcaa ccagatgccg tgaagacaag
     1261 aaagcagggc cggatcaggc cgtggatcgg ggagccgccg cgtgaggtgg cgccggaggt
     1321 cacgggctac cggctggata gccatcagcg cttcgacatg ttgcaggggt cgatcgcggg
     1381 gatggtgctg atcccgttct ggttcgtctt cttcagcgtg ttgatcggtc tggtgggcgg
     1441 tcgttacatg ggcgagttcg ccatcaccat tgccaacatg ctgctgggag cggtcattgc
     1501 gctggtcgtg gtgccggccc tgcacgaggc ggttcacggg ctggcggcga tgctgtgcgg
     1561 cgttcggccg tcgtatggga tcggccccgg cttcgcctac accaccttcc ccgatccggt
     1621 cggcaaacgc gcctatctcg cgatcgggct ggcgccgctg gttgtcctgt cgctgattgg
     1681 cttcccgctg gccgccgttt gggacagtgg cgccggggcg gtcctcttct tcatggtcgt
     1741 gaatgcggcc ggcgcggttg gcgacctctg gatgtcgtgg cggatcgcgc tccagccgcc
     1801 ccacgcgctg ttcgtcgatc tcgccgacgg tttcgcggtt cttattccag acgctcccga
     1861 acccgtcgct ccataatccg ctcgaagcta tccaggatgc tttggagccc gtccttgaac
     1921 cacgagttcg gccgccgaaa ctaactcgtg ccgaccggct cgtggtaaag caactggact
     1981 gcaccgtcgc cgcactggac cacgacgcca tccgcgcgcc ggtcgatgac actgcccggc
     2041 tggtgcccat cgcctcccgc gtccggcatc agccgcgctt tcaggatgcg cgccgggacg
     2101 ccatcgaccg tcccgaatgc tccgggcggg tcgacaaacg ccgagagacc gcgaatctgg
     2161 ttgtagatat gcgcggcagg gctattccag tcgatgacgc gcaactcgtc cgggatggca
     2221 ccgtagtagg acgcctgcga ctcgtcctgc ggctcgcctg gatcaccggc gatgactttt
     2281 tcgagggcag tggcaatgac gcccggcgcc tggctaaaga gcttttgcat gaaggacgcg
     2341 aggtcatcgt cgtcgctgac ctcgatggcg ac
//
LOCUS       DASUGJ010000524         6282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_83081, whole genome shotgun sequence.
ACCESSION   DASUGJ010000524 DASUGJ010000000
VERSION     DASUGJ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83081"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1677)
                     /locus_tag="VFV93_11845"
     CDS             complement(67..1677)
                     /locus_tag="VFV93_11845"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX5166082.1"
                     /translation="MVQATERGVSRHGLENHGVRPGGAVHWNQTAPLLYEEAIRRGDG
                     ELAFGGPLSVTTGEYTGRSPKDKFIVREPSSEANVWWGSVNQPFDPASFDRLHERVAE
                     YLSGRELFVQDLNVGADPTYRMPLRVVSESPWHALFARTMFIRPSVAELEDHVPQFTI
                     LHAPYMQADPERDDTRSHTFIIVNIAKGLILIGGTEYGGEIKKSIFSIMNFLLPLRDV
                     MSMHCSANVGPDGDVALFFGLSGTGKTTLSSDPSRTLIGDDEHGWSDDGVFNFEGGCY
                     AKVINLSADAEPEIYAATRSFGTVLENCVLDEVTREIEYENDSLTENTRAVYPLDIIS
                     NASRTGSAGHPRNIVLLTADAFGVLPPIARLTPEQASYYFLSGYTAKVAGTERGLTEP
                     EATFSACFGDVFLLLRPNVYGRLFKEKIRKHQPDVWLVNTGWTGGAYGVGHRISIAYT
                     RAIIDAALSGALKNVAYQEDPNFGIQVPTEVPGVPSEVLIPSNAWADRAAYDQAAIKL
                     AARFHENFKNYADDVDPEIREAGPNVGR"
     gene            complement(1796..3301)
                     /locus_tag="VFV93_11850"
     CDS             complement(1796..3301)
                     /locus_tag="VFV93_11850"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX5166083.1"
                     /translation="MSSDMTIAAAELSHETNTFSSVPTDLQAFQRAGLRRGAEIRASL
                     RDSATSFAGFFDGAEKHGYLLVPIIAVWATPSGMVTEEALAGIVNDLIAGIAATRPDG
                     VLLALHGAMVSAIDDDADGWILEQVRGTVGKDVPIVATLDLHANISQRMVDCADVLLG
                     YDTYPHVDQRERAVDASDLIVRLIRREIRPTSSLVKPPMMPTSQKMTTASEPMKSIMA
                     RAEEIESRKGVLNVTVAGGFPPANTPDTGLSVLVTTDNDPALAEAAAAELARFAWELR
                     DQFLGGVASWEDAADAIRGNPGGPVVLVDIGDNPWTGGPGDSVELLRFLLNEGVDGAA
                     LASIVDPQAVKRCVAAGPGATVRLDLGGHTDGRHGEPLEVEGYVRLISDGRYRNLGPM
                     HAGVEVKLGPTVVLVVGGVEVLVTTFPETPIDLNVFRAHGIEPTSRRVIALKGKGHFR
                     AAFEPIASRVVLVEGPGITGADLQRLEFTKLRRPIWPIDAPVQWEQGDSAV"
     gene            complement(3298..4146)
                     /locus_tag="VFV93_11855"
     CDS             complement(3298..4146)
                     /locus_tag="VFV93_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5166084.1"
                     /translation="MDKLIISVATTGSATMRSQTPHVPISEEEIAQQVVDSWRAGAAI
                     AHIHVRNEREIVTCDPARYQKVTDLVRAAGCDIVMNWSTGGGAGVTTDEERAASIRLS
                     PEVASFDCGSTNFGKGVFINSPSFLDELANSMNEHLVTPEIEIFDTGMIENARDLIDR
                     GLLTPPHWFQFVLGVRGGAPATARELTHMVDSIPAGALWSVCAIGRNQLPMNMIAMAM
                     GGHVRTGLEDNIYYGYRRLATGNPQLVERLARLARELGRELASPDDARQMIGVAARRG
                     GNGLSA"
     gene            4366..4545
                     /locus_tag="VFV93_11860"
     CDS             4366..4545
                     /locus_tag="VFV93_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166085.1"
                     /translation="MNLFSRMIRSLQQSSIMDRGFETYYGALVRSQSHGGPTASEARR
                     DFLAARRSMDGIGIL"
     gene            4672..4745
                     /locus_tag="VFV93_11865"
     tRNA            4672..4745
                     /locus_tag="VFV93_11865"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4706..4708,aa:Glu,seq:ctc)
     gene            4907..6253
                     /locus_tag="VFV93_11870"
     CDS             4907..6253
                     /locus_tag="VFV93_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5166086.1"
                     /translation="MPHEELKRRVLEEIDRNADHLIEVGDRIYRDPELGFKEFRTSKI
                     VATEFQRLGLGYHEDLAITGLKTLVNGGSAGPTVGVIGELDSVLVADHEFANPETGAA
                     HCCGHNAQLMTMLGVANGLIRSGVINELAGNVAFFAVPAEEYVEIDYRAQLRREGKTE
                     FLGGKSEMIRLGAFDDVDMAMMVHGASSQNMQAVAGVSSSNNGFVGKQARFIGKAAHA
                     GGAPHRGINSLYAAEIALASINAQRETFKDEDTVRIHPILTRGGDLVNVIPNDVRFET
                     MVRGKTADAIEDAGRKVDRALRAGAVALGAQVEITTLPGYMPLFNDRQMADLFKANFL
                     TFYGEDDWEETGHRTGSTDMGDIAHIMPALHPHVNGFSGTGHGSDWAIDDKYLVYVLP
                     AKLMAMTVIDLLAGSGAKAKEIIDTVKPPMTRDEYLAFMRRNAREELFDGAAVGPS"
BASE COUNT         1077 a         2101 c         2019 g         1085 t
ORIGIN      
        1 aatcaatgcc ccactccggt acgggcaata ttgcccgtac cggagtgggg cattgattgt
       61 ggtgcattac ctgccgacgt tcgggccggc ttcgcggatc tccggatcga cgtcgtcggc
      121 gtagttcttg aagttctcgt ggaagcgggc ggcgagcttg atggcggcct ggtcgtaggc
      181 cgcgcggtcg gcccaggcgt tgctgggaat cagcacctcg ctcggcacgc cgggaacctc
      241 ggtcggcacc tggatgccga agttgggatc ctcctggtag gcaacgttct tcaaggctcc
      301 gctgagcgcc gcgtcgatga tcgcgcgtgt ataggcgatc gagatgcggt ggccgacgcc
      361 gtaggcgccg ccggtccagc cagtgttcac cagccagaca tccggctggt gcttgcggat
      421 cttctccttg aacagccggc catagacgtt gggccgcagg agcaggaaga catcgccgaa
      481 gcaggccgag aaggtcgcct ccggctcggt caacccgcgc tcggtcccgg cgaccttggc
      541 cgtgtagccc gagaggaagt agtacgacgc ctgctccggt gtcagtctgg cgatcggcgg
      601 caggacgccg aacgcgtcgg ccgtcagcag gacgatgttg cgcggatgcc ctgctgaacc
      661 ggtccgtgac gcgttcgaga tgatgtcgag cgggtagacg gcgcgggtgt tctcggtcag
      721 cgagtcgttc tcgtactcga tctcgcgcgt gacctcgtcg agaacgcagt tctcgagcac
      781 cgttccgaac gagcgggttg ccgcgtagat ctccggttcg gcgtcggccg agaggttgat
      841 gaccttggca tagcaaccgc cctcgaagtt gaagacgccg tcgtcggacc aaccatgctc
      901 gtcgtcaccg atcagtgttc ggctggggtc ggatgagagc gtcgtcttgc cggtgccgga
      961 gaggccgaag aacagcgcga catcgccgtc cgggccgacg ttggccgagc agtgcatcga
     1021 catcacatcg cgcagtggca gcaggaagtt catgatcgag aagatcgact tcttgatctc
     1081 gccgccatac tctgtgccgc cgatcaggat cagccccttg gcgatgttaa cgatgatgaa
     1141 cgtgtgcgag cgcgtgtcgt cccgctcggg atccgcctgc atgtatggcg cgtggagaat
     1201 cgtgaactgg ggaacgtggt cctcaagctc ggcgaccgac gggcggatga acatcgtccg
     1261 ggcaaatagg gcgtgccagg gactctcact gacgacgcgc agcggcatcc ggtaggtcgg
     1321 gtcggcgccg acgttcaggt cctgaacgaa gagttcgcga ccgctgagat actcggcaac
     1381 ccgttcgtgc agccggtcga agctggccgg atcgaacggc tggttgaccg agccccacca
     1441 gacgttggcc tcactcgacg gctcgcggac gatgaacttg tccttcggcg agcgaccggt
     1501 gtattcgccg gtcgtgaccg acaatggacc accgaaggcc agttcgccgt caccgcgccg
     1561 gatcgcctct tcgtagagca acggcgccgt ctggttccag tggaccgcac ccccgggccg
     1621 tacgccgtgg ttctccagcc cgtgcctgct cacacctcgc tcagttgcct ggaccatcat
     1681 ttccctctcc ttgacatcca cacgcgtccg ccggttcgct gaggatcggg tggcgatgtc
     1741 tggccggcac gacgtactcc cacggatgag atccctcgac gtttccgatc aagaatcata
     1801 ccgccgaatc accctgttcc cattgcaccg gcgcatcaat cggccagatt ggtctccgca
     1861 gtttcgtgaa ttcgagccgc tgtagatctg ccccggtgat gcccggcccc tcgaccagca
     1921 cgacccgcga cgcaatcggc tcgaacgccg cccgaaagtg ccccttgccc ttgagcgcga
     1981 tcacgcgcct gctggtcggc tcgatgccgt gcgcccggaa gacgttcagg tcgatcggcg
     2041 tctccgggaa cgtcgtcacc agcacctcga cgccgccgac gaccaggacg accgtcggac
     2101 ccagcttgac ctcgacaccc gcgtgcatcg ggccgaggtt gcggtagcgc ccatcggaga
     2161 tgagccgcac gtagccttcg acttccagcg gctccccatg gcggccatcg gtgtggccac
     2221 ccagatcgag gcggacggtc gcgcccggtc cggccgcgac gcagcgcttg accgcctgcg
     2281 gatcgacgat cgacgccagc gcggccccgt cgacgccttc gttgaggagg aagcgcagca
     2341 gctcgaccga atcgcccggc ccgcccgtcc acggattgtc gccgatgtcc accaggacga
     2401 ccgggccacc tgggttaccg cggatcgcat cggcggcatc ctcccaactc gcaacgccgc
     2461 cgaggaactg gtcgcgcagc tcccaggcga agcgcgccag ttcggcggca gcagcttccg
     2521 ccagcgccgg gtcgttgtcg gttgtgacga gaacgctcag accggtgtcg ggcgtgttgg
     2581 ccggcgggaa acccccggcc accgtcacgt tcaggacgcc cttgcggctc tcgatctcct
     2641 cggcgcgggc catgatcgac ttcatcggct cgctggcggt cgtcatcttc tgcgaggtcg
     2701 gcatcatcgg cggcttgacc agtgaggacg tcggccgaat ctcgcgccgg atcagccgca
     2761 cgatcaggtc ggaggcatcg acggcccgct cgcgctgatc gacatgcggg taggtgtcgt
     2821 agccgagcag gacatcggcg cagtcgacca tccgctggct gatgttggcg tgcagatcga
     2881 gcgtcgcgac gattgggacg tccttgccga ccgtcccgcg cacctgctcc agaatccagc
     2941 cgtcggcatc gtcgtcgatc gcgctgacca tcgcgccgtg aagggccaac aggacgccgt
     3001 cgggtcgcgt cgcggcgatc ccggcgatca ggtcgttgac gatgcccgcc agcgcctcct
     3061 cggtcaccat acccgacggc gtcgcccaga ccgcgatgat cggcaccagc aggtagccat
     3121 gcttctcggc gccgtcgaag aagccggcga acgaggtcgc cgagtcgcgc aggctagccc
     3181 gaatctccgc gccgcgccgc agacctgccc gctgaaatgc ctgcaggtcg gtcggcaccg
     3241 acgagaaggt gttggtctcg tgcgacagtt cggcggcggc gatcgtcatg tccgagctca
     3301 cgcgctgagc ccgttcccgc cgcgccgggc ggcgacaccg atcatctgcc gggcgtcgtc
     3361 agggctggcc agctcccggc ctagctcgcg ggccagccgc gccagccgct cgaccagttg
     3421 cgggttgccg gtcgcaagcc gccggtaacc gtaatagatg ttgtcctcca gcccggtccg
     3481 gacgtggcca cccatcgcca tcgcgatcat gttcatcggt agctgattgc ggccgatcgc
     3541 gcagaccgac cagagcgcgc cggccgggat cgaatcgacc atgtgggtca gctcccgggc
     3601 cgtcgccggg gcgccaccac gcacgcccag cacgaactgg aaccagtgcg gcggcgtcag
     3661 caggccacgg tcgatcagat cacgggcgtt ctcaatcatg ccggtgtcga agatctcgat
     3721 ctccggcgtc accagatgct cattcatcga gttggccagt tcgtcgagaa aggatgggga
     3781 gttgatgaag acgcccttgc cgaagttggt cgagccgcag tcgaagctgg caacttcggg
     3841 cgacagccgg atcgacgccg cccgttcctc gtcggtcgtc acaccggcgc cgccgcccgt
     3901 cgaccagttc atcacgatgt cgcagccagc tgcccggacc aggtcggtca ccttctggta
     3961 gcgcgcggga tcgcaggtca cgatctcacg ctcgttgcgg acgtggatgt gggcgatggc
     4021 tgccccggcc cgccaggagt cgacgacctg ctgggcgatc tcctcttcgg agatcggcac
     4081 atgcggcgtc tgcgaccgca tcgttgcgga tccggtcgtt gcgaccgaga tgatgagctt
     4141 gtccacccgt ggcgttccct tccccattca gccgcaatcg gcgtccagat gatagggcct
     4201 cacgacgctg gcgtgaaggg ttgattgggt ggttcgcgcg acctcggcca cgcggatcac
     4261 ccaaaagtca caacctctgg cagacgaatg tatattcact tgcacatatc tgcatatcat
     4321 gatatgatct agtcaacgtt ggtaaaggca ttggaggcaa ccaccatgaa cctgttctct
     4381 cgcatgatcc gctcgctgca gcagtcctcg atcatggacc gcggtttcga gacctactac
     4441 ggcgccctcg tccgttcgca gtcgcacggc ggcccgacag cgtctgaagc gcgccgcgac
     4501 ttcctggctg ctcgccgctc gatggacggc atcggcatcc tctagagcgt acgcagtcga
     4561 cgcgccggat cgctcgctca cgatcaatcc ggcatgcttg ccgggcagcg acggggtttg
     4621 acaccctgtc gcgggcgttg atatactccc gcccagtcaa gcatgacgcg tggcgcattc
     4681 gtctagcggc ctaggacacc gccctctcaa ggcggagatc aggggttcga atcccctatg
     4741 cgctactaca gcggctcccg cgattgcggg agccgctttt tgttccaccg ttccatgacg
     4801 gtatcgtcag gggcaccttg catcacccat cgtctgcttg acgatccgcc gccgctccgg
     4861 caccatcccc ggcgaggatt cgagatatgg gaggggtgga gcatcgatgc cacacgagga
     4921 actgaaacga cgggtactcg aagagatcga ccgtaatgcc gatcatctga tcgaggtcgg
     4981 cgaccggatc tatcgcgatc ccgaactggg tttcaaggaa ttccggacgt cgaagatcgt
     5041 ggcgaccgag ttccagcggc tggggctcgg ctaccatgag gatcttgcga tcaccggctt
     5101 gaagacactc gtcaacggcg gctctgccgg cccgaccgtc ggcgtcatcg gcgagctgga
     5161 ctcggtcctc gtcgccgacc acgagttcgc caacccggaa acgggcgcgg cccactgctg
     5221 cggccacaac gcccagctga tgacaatgct cggcgttgcc aacggcctga tccgttccgg
     5281 cgtcatcaac gagctggccg gcaacgtcgc cttcttcgcc gtcccggccg aggagtacgt
     5341 cgagatcgac taccgcgcgc agctccgccg cgagggcaag accgagttcc tcggcggcaa
     5401 gagcgagatg atccggctcg gcgccttcga cgatgtcgac atggcgatga tggtccacgg
     5461 cgccagcagc cagaacatgc aggcagtggc cggcgtcagc tcgtccaaca acggcttcgt
     5521 cggcaagcag gcccgcttca tcggcaaggc ggcccacgcc ggtggagcgc cacatcgcgg
     5581 catcaactcg ctctacgcgg ccgagatcgc gctggcctcg atcaacgccc aacgcgagac
     5641 gttcaaggac gaggacaccg ttcggattca cccaatcctg acccgtggcg gcgacctggt
     5701 caacgtcatc cccaacgatg tccgcttcga gacgatggtg cgcggcaaga cggccgacgc
     5761 gatcgaggac gccggccgca aggtcgatcg cgccctgcgg gccggggcgg tcgcgctcgg
     5821 cgcccaggtc gagatcacga ccttgccggg ctacatgccg ctcttcaacg atcgccagat
     5881 ggcggatctg ttcaaggcca atttcctgac cttctacggc gaggacgact gggaggagac
     5941 cggccaccgg accggctcga ccgacatggg cgacatcgcc cacatcatgc cggcgctcca
     6001 cccgcacgtg aacggcttct ccggcaccgg ccacggctcc gactgggcga tcgacgacaa
     6061 gtacctcgtc tacgtcctgc cggcgaagct gatggcgatg accgtgatcg accttctggc
     6121 cggcagcggc gccaaggcca aagagatcat cgacaccgtc aagccgccga tgaccaggga
     6181 tgaatacctc gccttcatgc gccgcaacgc ccgcgaggag ctgttcgacg gcgcggcggt
     6241 cgggccgtcg taagcagcgg gattcgggac tcgggattcg gg
//
LOCUS       DASUGJ010000525         4718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1472835, whole genome shotgun sequence.
ACCESSION   DASUGJ010000525 DASUGJ010000000
VERSION     DASUGJ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472835"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..3480
                     /locus_tag="VFV93_11875"
     CDS             337..3480
                     /locus_tag="VFV93_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5166087.1"
                     /translation="MIFRALRSASALALLFGLTGGYSTAIAQQTPAPSGPETTPQTGV
                     IDCDTNTPGVQLDCPTERIVVTGSNIAGAVESAALPVEVYTADDNLKSGNQSTMDFIK
                     TLSVVGSTVGETNQFQAGYGGIGAATLNLRGLGGGRTLTIFNGRRFSENTNMIPSIAL
                     SRTEILKDGGAVIYGADATGGVVNFITRDSFDGLIVQGDFRGIDGSKGDYSGGLLWGH
                     EFDQSNLMLSAEFSHRSELPVSKRDWATREYSENSTPWAPYHNYGTYLLQSATGTTLN
                     TPVADFTTQECQNSTGPVQGQVIPISGLPTCWWNYMIHTYNLVEEIDQVRLYGQFTAD
                     LDDSTRFTAQLSYGKSDANNIGTVAAYQANVGPAPSSGTAFQYRVPRANPYFDSFLAQ
                     NASQINPAILPLIHHADQFLSIMFGPSGNPAWGPGEGTTPTTQLENWNAVAALDGEWG
                     DWAGDWLDTWKVSMTYNLATTDNTLPDIVGYKVQQALNGFGGPNCNAPDLVPDNWNLV
                     ADANGDGTVSTAENRAYTEAFYASIGTQNPAAAGKNGCMYLNPFASSYAANGTFGTPN
                     PRYIPGNEIPAELAAWLYDERHEESVNNNLVIDALMSGGTPLNLPGGMVAWAAGAQWR
                     QSENRDEVEGEFLDPGEMPCQWPGQQPGQGGCPPTGESPYWFFGQNSPNKTDQQQYSY
                     FGELQAPVLDNLGFQLAVRREEFPRSGLGATVYKVAGKWDPFEWLAIRGSFGTNYATP
                     PNITPGDISTGLSLIANAGNKYLRVQTENLAGLKPETAEVMNFGAIFNFDDLPLDGSF
                     RLSLDYFNFEIKDEIKTVSHNAILNTVFAGARGSSQPINCSAALIGRISFINGVGAAG
                     CTQGVTIGQDVTSIRSVRGNGPGATTSGIDIDASYGFTALGGDWTASFQGTNVLDYTI
                     AAFDLNGVTLSPEVDGLGFANYSRDGDIISEWRSSTTLNYAVGNHNLRYVYRFIQGVK
                     DDRFLGTPFENIDDFATHNLYYQYTMPWDENFTATLAIDNVTDEDPPFTQQQYSYDPF
                     IGNALGRTIKIGLRKEF"
     gene            3578..>4718
                     /locus_tag="VFV93_11880"
     CDS             3578..>4718
                     /locus_tag="VFV93_11880"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5166088.1"
                     /translation="MSAIGPVDAAAAGMSAERLERIPAYFDSYIRKQKLPCCASLVAR
                     GGQVAHLSFQGTTEMGGSTPIGEDTIYRIYSMTKPVTSVAAMMLFEEGLLRLDHEVHR
                     YIPEFQDAMVLDGGTADAPKLRKPDRPMMVRDLFLHTSGLTYSFLQQGLADEIYRRRK
                     IEHIGWQQGDLKSFCTALAQAPLAFSPGDKWNYSNSTDVLGRVVEVASGMSLDQFFQK
                     RIFGPLGMKDTFFSVPDDKIGRLMACYARNPNTGAITQSDAAGKASSYAKPPNVLSGG
                     GGLASTIGDYFRFCSMLLAGGELNGVRILSPKTLEFMTMNHLPGGKTLKDMGDKTFSE
                     SRMDGSGFGLGWAVTTDVVATTQPGSMGTFSWGGMASTFFWIDPVE"
BASE COUNT          909 a         1511 c         1462 g          836 t
ORIGIN      
        1 cgttgcttcg aaaatctggc gtcgcgttgt agcggagcgg tcaacattgc tccggcgttc
       61 gcgctccttc cttaccggaa ttaccggcac agatcgtctg gcgactgacc gtcggttcga
      121 atctggcatt ccttcccctt ggaggacgct tgctggcttc gacctataca agcagcggcg
      181 cgggttgcga tccagaagca acaaccatct tttctaaaaa acaagccggt tggccgggtt
      241 gtcatgagtg aattactccg catagtgcgc gcggacggga gtttccgtca gcgggaggtc
      301 gcctgcgaga gcggccccaa ggggagtgaa ccactgatga tttttcgagc gctcagaagc
      361 gcgtctgcgc ttgcgttgct gtttggcctg acgggaggct attcgacagc aattgcccag
      421 caaacgccgg cgccttcggg tccggagacg acgccgcaaa ccggggtcat cgactgcgac
      481 accaacaccc ccggcgtcca gctggattgc cccaccgagc gcatcgtcgt gacaggctcg
      541 aacattgcgg gcgcagtcga atctgctgcg ctgccagtcg aggtttacac ggctgacgac
      601 aacctgaagt ccggcaacca gtccacgatg gacttcatca agacgctgtc tgtcgtcggt
      661 tcgacggtcg gcgaaacgaa ccagttccag gccggttatg gcggcatcgg cgccgcgacg
      721 ctgaacctgc gcggtctcgg tggcggccgg acgctgacca tcttcaacgg ccggcgtttc
      781 tccgaaaaca ccaacatgat cccgtccatc gccttgtcgc gaacggagat cctgaaagac
      841 ggcggcgcgg tgatctatgg cgccgacgcc accggcggcg tcgtcaactt catcacgcgt
      901 gacagcttcg acggcctgat cgttcagggc gacttccgcg gcattgatgg ctccaaaggc
      961 gactacagcg gcggtttgct gtgggggcac gagttcgacc agtcgaacct gatgctctcg
     1021 gcggagtttt cccatcgcag cgagttgcca gtcagcaagc gtgactgggc gacacgcgaa
     1081 tattccgaaa actcgacacc ctgggcgccg taccacaact acggcaccta cctgctgcaa
     1141 tccgccaccg ggacgacgct caacacgccg gtcgccgact tcacaacgca ggagtgccag
     1201 aactcgaccg gtccggtgca gggccaggta attccgatca gcggccttcc gacctgctgg
     1261 tggaactaca tgatccatac ctacaacctg gttgaagaaa tcgaccaggt gcgtctctac
     1321 ggtcagttta ccgcagacct cgacgactcg acgcggttta cggcgcagct gtcctacggg
     1381 aagtcggacg ccaacaatat cggcactgtc gccgcctatc aggcgaacgt tggtccggcg
     1441 ccgagcagcg gcacggcgtt ccagtaccgc gtcccgcgcg cgaacccgta cttcgactcg
     1501 ttccttgcgc agaacgccag ccagataaac ccggcgatcc ttccgctgat ccaccatgcc
     1561 gaccagttcc tctcgatcat gtttggtccg agcggcaatc cggcctgggg accgggcgaa
     1621 ggcacgacgc cgacaaccca gctcgagaac tggaacgcgg ttgccgcgct tgacggcgaa
     1681 tggggcgact gggcgggcga ctggctcgac acgtggaagg tgtcgatgac ctacaacctg
     1741 gcgacgaccg acaacacgtt gccggacatt gtcggataca aggtgcagca ggcgctcaac
     1801 ggcttcggtg ggccgaactg caacgcgccc gacctggtgc ccgacaactg gaacctggtg
     1861 gctgacgcca atggcgacgg cacggtttcg acggccgaga accgcgccta caccgaggcc
     1921 ttctacgcct cgatcggcac gcagaacccg gcggcggccg gcaagaacgg gtgcatgtat
     1981 ctcaatccgt ttgcgtcgag ctacgcggcc aacggcacgt tcggcacgcc gaacccgcgg
     2041 tacattcccg ggaatgaaat cccggcggaa ctcgcagcgt ggctctacga cgagcggcac
     2101 gaagagtcgg tgaacaacaa ccttgtcatc gacgctctca tgtccggcgg caccccgctg
     2161 aaccttccgg gcggcatggt cgcctgggcg gccggcgcgc agtggcgcca gtcggaaaac
     2221 cgcgacgagg ttgaaggcga gttcctggat ccgggcgaaa tgccttgcca atggcccggc
     2281 cagcagcccg gacaaggcgg ctgtccgccg accggcgagt cgccctactg gttcttcggc
     2341 cagaacagcc cgaacaagac cgatcagcag cagtactcct acttcgggga attgcaggcg
     2401 ccggtgctcg acaatctcgg cttccagctt gccgtgcgtc gcgaagaatt cccgcgttcg
     2461 ggactcggcg cgaccgtcta caaggtcgcc ggcaaatggg accccttcga atggctcgcc
     2521 atccgcggtt cgttcggcac caactatgcg accccgccga acatcacgcc gggcgacatc
     2581 tcgaccggac tttcgctcat cgccaatgcg ggtaacaaat acctgcgggt ccagaccgag
     2641 aaccttgccg gactcaagcc ggaaacggct gaggtcatga acttcggcgc gatcttcaac
     2701 ttcgacgacc tgccgctcga cggctcgttc cgcctctcgc tcgactactt caacttcgag
     2761 atcaaggacg agatcaagac tgtcagccac aacgcgatcc tcaacaccgt gttcgccggt
     2821 gcgcgtggca gctcgcagcc gatcaactgc tcggcggcgc tgattggacg gatctcgttc
     2881 atcaacggcg tcggcgctgc gggttgcacc cagggcgtga ccatcggtca ggatgtcacc
     2941 tcgatccgtt cggttcgcgg caacggtcct ggcgccacga cgtcgggcat cgacatcgat
     3001 gcgagctatg gcttcacggc gctcggcggc gactggacgg cgagcttcca gggcaccaac
     3061 gtgctcgact acacgatcgc ggcgttcgac ctgaacggcg tgacgttgtc tccggaagtg
     3121 gatggtctcg gctttgcgaa ctactcgcgc gatggcgaca tcatctccga gtggcgttcg
     3181 agcacgacgc tgaactacgc ggtcggcaac cacaacctgc gctacgtcta tcgcttcatc
     3241 cagggcgtga aggacgaccg gttcctcggc actccgttcg agaacatcga cgacttcgcg
     3301 acccacaacc tctactacca gtacaccatg ccgtgggatg agaacttcac ggcgaccctc
     3361 gcgatcgaca acgtgaccga cgaggatccg cccttcaccc agcagcagta cagctacgat
     3421 ccgttcatcg gcaacgcgct gggacggacc atcaagatcg gtcttcggaa ggaattctga
     3481 taccgatacc ccgagagact gcccccggcc gcgaggtccg ggggctgttt ttttcgggca
     3541 gcggctgcgc taaggatagg tccgcaaagg agcctccatg agcgccatcg gaccggtaga
     3601 cgcagcggca gcaggcatga gcgcggagcg cctggagcgc atcccggcct atttcgacag
     3661 ttacatccgg aagcagaagc tgccctgctg cgcctcgctg gtggcgcggg gcggacaggt
     3721 ggcgcacctc tccttccagg ggacgaccga aatgggcggg tcgacgccca tcggcgagga
     3781 cacgatctac cgcatctatt cgatgaccaa gccggtgacc tcggttgcag cgatgatgct
     3841 gttcgaggaa ggcctgctgc ggctcgacca tgaggtccat cgctacattc ccgaattcca
     3901 ggacgcgatg gtgcttgatg gcggcacggc ggatgcgccg aagctgcgca agcccgaccg
     3961 gccgatgatg gtgcgggacc tgttccttca cacatccggc ctgacctact cgttcctgca
     4021 gcagggcttg gccgacgaga tctaccgcag gcggaagatc gaacacatcg gctggcagca
     4081 aggcgacctg aagtcgttct gcaccgcgct ggcgcaggcg ccgctggcgt tctcgccggg
     4141 cgataagtgg aactactcca actcgaccga tgtgttgggc cgcgtggtgg aagtggcctc
     4201 cggcatgagc ctcgaccagt tcttccagaa gcgcatcttc gggccgctgg gaatgaagga
     4261 cacattcttc tccgtgccgg acgacaagat tggccggctg atggcctgct atgcgcgcaa
     4321 tcccaacacg ggcgccatca cgcagtccga tgcggcgggc aaggcgagtt cctatgcgaa
     4381 gcctccgaat gtcctgtcgg gcggcggcgg gctggcgtcc accattggcg attatttccg
     4441 tttctgctcg atgctgctgg cgggagggga gctcaatggc gtccgcatcc tgtcgcccaa
     4501 gacgctggag ttcatgacca tgaaccacct gccgggcggc aagacgctga aggacatggg
     4561 cgacaagacc ttctcggaat cccgcatgga cggctcgggc ttcgggctcg gctgggcggt
     4621 gacgaccgac gtggtggcga cgacgcagcc gggatcgatg ggcacattct cctggggcgg
     4681 catggcctcg accttcttct ggatcgatcc ggtggaag
//
LOCUS       DASUGJ010000526         6304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1472854, whole genome shotgun sequence.
ACCESSION   DASUGJ010000526 DASUGJ010000000
VERSION     DASUGJ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472854"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..922
                     /locus_tag="VFV93_11885"
     CDS             347..922
                     /locus_tag="VFV93_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5166089.1"
                     /translation="MVDAQGRGPGSVDELDLRIIGALETDGRRPVADIARELGVPKST
                     VQRRLDALIRERVILVAAYADSGKLGLGIHAHLNMRVDLASYQQAIDKVRQLTEVRWL
                     AVTTGRDDLTAEGYFASPTHLHEFIRDKLAPIPGIIAVETAIILSVEKLTFHWGDLLR
                     EAADHVNPHLRPSALPESFQRVTAPTGSHVP"
     gene            1016..2812
                     /locus_tag="VFV93_11890"
     CDS             1016..2812
                     /locus_tag="VFV93_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5166090.1"
                     /translation="MNEQWLNSLRYHLEARQIDRRGFLKRAAVVGMGIPAVSAILAAC
                     GGDDDDDDDGGSTSTTGSGSTEPTATRSGVTVNQNQTPTPATSASPGTSASPGTSSPE
                     ATSTTAAPTGAAGGSVTFARAVDAENMDPVTQDGNINIWILMSIYDQLIRVDEQGTGL
                     LPGLATDWEVSDDALTYTFSIREGVKFSDGSDLTVEDIKWSIERARDTEDSPWTFTLV
                     QAADIQTPDDTTVVITLSEPWSPFLSDISMFNAAIVSKAFADSVGVESLVDQCMGTGP
                     FALKEWNKAVSMTLVKNENYWEEGLPLLDEIVFTVVPDGNSEILQLQGGEIDGIIGQG
                     DVPFNRIQELDADENIQVIQSLSTYNNFMVLQTQHEPLDDVHVRRALNYATNKQALID
                     TILFGAAELSNSFMPNGALYWNPDQEGYEYDIDKAKEEMAQSKVPDGFNVEIQIRSGN
                     DQQSAIATALQAMWADIGVTLDISPLEATVARDNYRAEKFQIQITGWTNDIIDPDELV
                     SYAILPDSTNNYHTGWLSQQAVDLANQGRAEQDDAARREIYYQIQQIHRDEAPWVYLY
                     VVPYVDALSANVKGFFHHPMGQYDFRNTSIES"
     gene            2955..3893
                     /locus_tag="VFV93_11895"
     CDS             2955..3893
                     /locus_tag="VFV93_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166091.1"
                     /translation="MGRGQYILRRLVQMIPVLLGITIIIFFLIRAIPGDPAIAMLGER
                     ATDDALARMRHSMGLDRPIYVQYAYFMRDLAQFDLGKSTRYDVPVSEILFTRLKVSLF
                     VVLYTTILTTIISVPLGVLAALRKDSVFDNIVRSTLMITLVMPSFWVGIMLIIIFSVK
                     LGIFPVSGYGEGFFGHIKHLFLPSLTVSLTIAPILIRSLRNSILEVLNQDYVKTARAK
                     GLREQTVISAHVLRNALIPAVTLLGISIGGLMGGTVITEKVFALPGAGALLIDSITAR
                     DYAMLQSATLIFACMVILVNLATDIIYSFLDPRVRF"
     gene            3950..4837
                     /locus_tag="VFV93_11900"
     CDS             3950..4837
                     /locus_tag="VFV93_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166092.1"
                     /translation="MSANVTEIGALSASVTPARKPRSAWDRARHNRNLMIGTSLLGLL
                     ILIALLAPVIAPYDPFEQDFNNTLRSPSLSHPFGTDNLGRDIFSRVIHGARIDLKVGI
                     VSVISPFVIGLLLGSLAGYFGGWTDTVVMRIVDIVQAFPFLVLVIAIVAVRGPGLQNM
                     YIAVALVAWIVYARLVRGEILVEKHKEYVTAAKAIGGSDWRIMRRHLLPNVITTSVVY
                     AMADIALYILLAAALSFLGLGAKPPSPEWGVMITEGRQFMTTAWWMSALPGVAIVITG
                     IILSLIGDGLADFLRPERR"
     gene            4869..5678
                     /locus_tag="VFV93_11905"
     CDS             4869..5678
                     /locus_tag="VFV93_11905"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5166093.1"
                     /translation="MAGGTLQDKVAIVTGAASGIGRAIAIRYGREGAVVHVADVNETG
                     GEETAAMIRDGGGRATFHRCDVSDPQQVQAMIDGAAADSGRLDIIVNNAGVGGTGDAV
                     AEIPVEAFQRVIGINLLGPFYGAKFAIPHLRRAGGGAIINIASTYGLIGAPNTPAYAA
                     AKGGLVMLTRQMAVDYSHEGIRVNAICPGFVDTDLGGRRARMSPEDAARALAAREARA
                     AIQPIGRQAQPDEMAGAAVFLASDDASFVVGAILTVDGGQTILHNWGQYYE"
     gene            5671..5874
                     /locus_tag="VFV93_11910"
     CDS             5671..5874
                     /locus_tag="VFV93_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166094.1"
                     /translation="MSSSEATHANATALARAAGLELSPDEMRPLADLYARYSGDRAAL
                     TAASLGEAEPATIFNAPKPDDGA"
     gene            5871..>6304
                     /locus_tag="VFV93_11915"
     CDS             5871..>6304
                     /locus_tag="VFV93_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5166095.1"
                     /translation="MTALDYLSIGELVEQLDGGERSSREVTEAQLARIGSMDGSLNAY
                     ITVMSEQALAAADRADRRRRGGERGSLLGVPLAVKDLFATAGVRTTAGSRVLADSVPE
                     STATAVQRLEDAGAVILGKTNMMEFAYGYPHPDYGETRNPWDT"
BASE COUNT         1146 a         2100 c         2017 g         1041 t
ORIGIN      
        1 tccctctcac ggctcccatc ccgtccctgc gaggtggagt gggctggtgt gttgaattcc
       61 gccgccggtg gaccagcgtc aggcgatatc gcggatgatg tagtaggtcg ccagaaaggt
      121 gcgataggcg gccagcgcgt cgggtgattc gaagcgtacc gttcggtctc cggtccgttc
      181 gacggtcggg atcagcgccg cgacagggcc atgacgaagt cgtgggcgat ttgcccacga
      241 cttcgctggc ttacaggcaa atcggccgaa attcggcggc ggattgacac aacggcccag
      301 ctcgggtacg atgcgccagt cgtgaaagcg gaacgcggaa gtgatgatgg tggacgcaca
      361 gggccggggg ccgggcagtg tcgatgagct tgacctccgg atcatcggcg cgctggaaac
      421 tgacgggcga cgacctgtcg cggacatcgc gcgcgaactc ggtgtgccga agtcgaccgt
      481 ccagcgacgg ctcgacgcgc tgatccggga acgggtcatt ctggttgccg cctacgccga
      541 tagcggcaag ctgggacttg ggatccacgc ccacttgaac atgcgggtgg atctggccag
      601 ctaccagcag gcgatcgaca aggtcagaca gttgaccgag gtgcgctggc tggccgtgac
      661 aaccgggcgg gacgatctga cggcggaggg gtacttcgca tcgccaaccc atctgcacga
      721 gttcatccgc gacaagctgg cgccgatacc gggcatcatc gccgttgaga ccgccattat
      781 tctcagcgtc gagaagctca cgttccactg gggagatctg ctgcgggagg ccgccgacca
      841 cgtcaacccg catctgcgcc ccagcgcgtt gcccgagtcg ttccagcgcg tcaccgcccc
      901 gaccggcagc catgttccgt gacggcccgg ttccgctgct accgcctcgc gtgtacccgg
      961 tcaacccgcc gttccacgaa ccacgaggag ttgaggagaa gaaagggagt gcaacatgaa
     1021 tgagcagtgg ctgaacagtc tcaggtacca cctcgaagcc cgtcagatcg acaggcgtgg
     1081 ctttctcaag cgcgcggcag tcgtcggcat gggtattccg gccgtctccg ctatcctggc
     1141 cgcctgcggt ggcgacgatg acgatgacga cgatggtggc agcacgtcga ccaccggcag
     1201 cggaagcacc gagccaaccg caaccaggag cggagtcacc gtcaaccaga accagacccc
     1261 aaccccggcc accagcgcca gccccggcac cagcgccagc cccggcacca gcagcccgga
     1321 ggcgaccagc acgaccgcgg ctccgaccgg cgcagccggt ggcagcgtca ccttcgcccg
     1381 ggccgttgac gcggagaaca tggaccccgt cacccaggac ggcaatatca acatctggat
     1441 tctgatgagc atctacgacc agctgattcg cgtcgatgag cagggcaccg gcctgctccc
     1501 cggcctggcg accgactggg aggtctccga cgacgcgctg acctacacct tctcgatccg
     1561 tgagggcgtc aagttctctg acggcagcga cctgaccgtc gaggacatca agtggtcgat
     1621 cgaacgggca cgcgacacag aagatagccc gtggaccttc acgctggtcc aggcagcgga
     1681 tatccagaca ccagacgata cgaccgtcgt catcacgctc agcgagccgt ggtcgccgtt
     1741 cctctccgac atctcgatgt tcaatgccgc gatcgtctcg aaggcattcg ccgactcggt
     1801 cggcgtcgag agcctggtcg atcagtgcat ggggaccggc ccgttcgcgc tcaaggagtg
     1861 gaacaaggcc gtctcgatga cgctggtgaa gaacgagaac tactgggagg aaggcttgcc
     1921 cctcctggat gagatcgtct tcacggtcgt gccggatggc aacagcgaga tcctccagct
     1981 ccagggcggc gagatcgacg gcatcatcgg ccagggcgat gttccgttca accggatcca
     2041 ggagctcgac gccgacgaga acatccaggt gatccagtcg ctctcgacct acaacaactt
     2101 catggtgctc cagacgcagc atgagccgct ggacgacgtc catgtccgcc gggcactgaa
     2161 ctacgcgacc aacaagcagg cactgatcga cacgatcctg ttcggcgcgg ccgagctctc
     2221 caactcgttc atgcccaacg gcgccctcta ctggaacccc gatcaggagg ggtacgagta
     2281 cgatatcgac aaggccaaag aggagatggc ccagtcgaag gtgccggatg gcttcaacgt
     2341 cgagattcag atccgctccg gcaacgacca gcagtcagcg atcgcgacgg cgttgcaggc
     2401 gatgtgggcc gacatcggcg tcacgctcga tatctcacca ctggaggcca ccgtcgcgcg
     2461 tgacaactac cgcgccgaga agttccagat ccagatcacg ggctggacga acgacatcat
     2521 cgacccggac gagctcgtct cgtacgcgat cctgccggat tcgaccaaca actaccacac
     2581 cggctggttg agccagcagg ccgtcgatct cgccaaccag ggccgcgccg agcaggacga
     2641 cgccgcccgg cgggagatct actaccagat ccagcagatc caccgcgacg aagcgccgtg
     2701 ggtctacctc tacgtcgtgc catatgtgga cgcgctcagc gcgaacgtca aggggttctt
     2761 ccaccatccg atggggcagt acgacttccg caacacctcg atcgagagct agcccccacc
     2821 ggcacagcaa ctgaggcgcc gggtagtctt gatgggatcg cccggcgcct cggggcggga
     2881 gcggagacac cacccgcgac cagcgtatcc gcgggagccg gaaccagccg gctcccgctc
     2941 cagaggagtg ttccatgggt cgaggtcagt acatcctgcg gcggctcgtc cagatgatcc
     3001 cggttctgct gggtatcacc atcatcatct tcttcctgat ccgggcgatt ccgggcgacc
     3061 ccgccatcgc catgctcggc gagcgtgcga cggacgacgc cctcgcgcgg atgcgtcaca
     3121 gcatggggct cgaccgcccg atctacgtcc agtacgccta cttcatgcgc gatctggcgc
     3181 agttcgacct cggcaagtcg acccgctacg acgtgcctgt ctcggagatc ctcttcacgc
     3241 ggctgaaggt ctcgttgttc gtcgtcctct acacgacaat cctgacgacg atcatctcag
     3301 tgccgctcgg cgtcctggcg gcactgcgca aggacagcgt cttcgacaat atcgtccgct
     3361 cgacactgat gatcacgctc gtgatgccct cgttctgggt cggcatcatg ctgatcatca
     3421 tcttcagcgt gaagctgggt atcttccctg tctccggcta cggcgaaggg ttcttcggcc
     3481 acatcaagca tctcttcctc ccctcgctga ccgtctcgct gacgatcgcg ccgatcctga
     3541 tccgctcgct gcgcaacagc atcctggagg tgctgaatca ggactatgtc aagacagcac
     3601 gtgccaaggg attgcgcgag cagacggtca tctccgccca tgtcctgcgc aatgcgctga
     3661 ttccagccgt gacgctgctg gggatcagca tcggcgggct gatgggcggg acggtgatca
     3721 ccgagaaggt gttcgcgctg ccgggggccg gggcgctcct gatcgactcg atcacggccc
     3781 gtgattacgc gatgttgcag tcggcgacgc tgatcttcgc ctgcatggtc attctcgtca
     3841 atctggcgac cgacatcatc tactcgttcc tcgacccgcg cgtccggttc tagcgcgggg
     3901 acacatcgtc gactgacgga ggcacgacag cgaagaagga ctgagcacga tgtccgcgaa
     3961 cgtcaccgaa atcggcgccc tctcggccag tgtcacgccg gcgcgcaagc cacgctccgc
     4021 ctgggaccgt gcccgccaca accgcaacct gatgattggc acatccctgc tcgggctgct
     4081 gatcctgatc gcgctgctgg cgccggtcat cgcgccgtac gatccgttcg agcaggattt
     4141 caacaacacg ctgcgctcac cgagcctgtc gcacccgttc ggaaccgaca atctcggccg
     4201 tgacatcttc tcgcgggtca ttcacggtgc gcggatcgac ctgaaggtcg gcatcgtcag
     4261 cgtcatctcg ccgttcgtga tcgggctgct gctcggctcg ctggccggtt acttcggcgg
     4321 ctggaccgac acggtcgtga tgcggatcgt cgatatcgtc caggcgttcc cgttcctcgt
     4381 cctcgtgatc gcgatcgtcg ccgtccgtgg ccccggcctg cagaacatgt acatcgcggt
     4441 cgcgctcgtg gcctggatcg tctacgcacg gctggtgcgt ggcgagatac tggtcgagaa
     4501 gcataaagag tacgtcactg ccgccaaggc gatcggcggt agcgactggc gcatcatgcg
     4561 ccggcacttg ctgccgaacg tcatcacgac ctcggttgtc tacgcgatgg ccgacatcgc
     4621 cctctacatt ctgctggcgg cggcgctctc gttcctgggt ctcggcgcga agccgccctc
     4681 cccggagtgg ggcgtgatga tcaccgaggg ccgccagttc atgacgaccg cctggtggat
     4741 gagcgcgctg ccgggcgtcg cgatcgtcat caccggcatt atcctgagcc tgatcggcga
     4801 cggcctcgcg gacttcctgc ggcccgaacg ccgatagcag cgtctacagg tattggggag
     4861 gggaaacaat ggcaggtgga acacttcagg acaaagtcgc gatcgtcacc ggcgcggcgt
     4921 cggggatcgg ccgggcgatc gccattcgct acggccgcga gggcgcggtc gtccacgtcg
     4981 ccgatgtcaa cgagacgggt ggcgaggaga cggccgcgat gatccgcgac gggggcggcc
     5041 gggcgacgtt ccaccgctgc gatgtgtccg acccgcagca ggtgcaggcg atgatcgacg
     5101 gcgcggcggc cgacagcggc cggctcgaca tcatcgtcaa caacgccggc gtcggtggaa
     5161 ccggcgatgc cgtcgccgag attccggtcg aggccttcca gcgcgtgatc ggcatcaacc
     5221 tgctcggccc gttctacggc gcgaagttcg ccatccctca cctgcgccgt gccggtggcg
     5281 gcgcgatcat caacatcgcc tcgacctacg gcctgatcgg cgcaccgaac acgcccgcct
     5341 acgccgccgc caagggcggg ctggtcatgc tgacccgcca gatggcggtc gactattcgc
     5401 acgaaggtat ccgcgtcaac gcgatctgcc ccggattcgt cgacaccgac ctcggcggga
     5461 ggcgggcgcg gatgtcgccg gaagacgccg cccgcgcgct ggctgcccgc gaggcacggg
     5521 ccgcgattca gccgatcggc cgtcaggctc agccggacga gatggccggc gcggccgtct
     5581 tcctcgccag cgacgacgcc tcgttcgttg tcggcgcgat cctgaccgtc gatggcggcc
     5641 agacgatcct ccacaactgg ggtcagtact atgagtagta gcgaggcgac acacgcgaac
     5701 gccacggcgc tggcccgtgc cgccggtctt gagctatcac cggacgagat gaggccgctg
     5761 gcggacctct atgcgcgcta ctcgggtgac cgggccgcgc tgacggcagc gtcccttggc
     5821 gaggcagagc cagcgacgat cttcaacgca cccaaaccgg acgatggcgc atgaccgcgc
     5881 tggactacct cagcatcggc gaactcgtcg aacagctcga cggcggagaa cgctcgtcgc
     5941 gcgaagtgac cgaggcgcag ctggcgcgga tcggcagcat ggacgggtcg ctgaacgcct
     6001 acatcaccgt catgagcgag caggcgctgg cggcggccga ccgcgccgac aggcgaaggc
     6061 ggggcggcga acgggggtcg ctgctgggtg tgccgctggc ggtgaaagac ctcttcgcaa
     6121 cagccggcgt ccggacgaca gccggctcac gcgtcctggc tgactcggtt ccggagtcga
     6181 cggcgaccgc cgtccagcgg ctggaggatg ccggcgccgt catcctcggc aagaccaaca
     6241 tgatggagtt cgcctacggc tacccgcacc ccgactacgg cgagacgcgc aacccgtggg
     6301 acac
//
LOCUS       DASUGJ010000527         4853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_83424, whole genome shotgun sequence.
ACCESSION   DASUGJ010000527 DASUGJ010000000
VERSION     DASUGJ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83424"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1274)
                     /locus_tag="VFV93_11920"
     CDS             complement(84..1274)
                     /locus_tag="VFV93_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5166096.1"
                     /translation="MATIRLSDGEYARRREAILASLGERGIGGLVLFNPNKMSYFSRF
                     TFIQTERPMAYVLTPTRSELLVPKLETEHAQEYAIVDEVVVYPEYPGERHPLEFLKDM
                     LVGLGLGSGKLAVDSDGYGQVYGYRGPKVSELLPEVEIVNVMDEIEYMQMINSEEELT
                     LIRESCKWGNLAHEYLQQGCAVGAGETEIALRASLEASLEMMEALGSEYRPMTSGFPA
                     MATFRGQVGKDSALPHAISSNVKLQKGDVLVTGAGAAVWGYGSELERTMVMGEPSKEQ
                     ERWFGLMLGAQTLALDTIKPGITCAAVDRELMRYFKEHGIQENWRHHTGHAKSMLIHE
                     APFLDIGDERVIEVGMVFTVEPGIYVPGFAGFRHSDTVVVTETGIESLTYYPRDLESL
                     TIPC"
     gene            complement(1307..1534)
                     /locus_tag="VFV93_11925"
     CDS             complement(1307..1534)
                     /locus_tag="VFV93_11925"
                     /inference="COORDINATES: protein motif:HMM:NF027067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HEX5166097.1"
                     /translation="MNCVICKHGETVDGTATVTLTRGAMTIVFRDVPARVCDTCGEEY
                     VDEAVAEQLLRSADASAKAGIQVEVRTFIAA"
     gene            complement(1815..>4853)
                     /locus_tag="VFV93_11930"
     CDS             complement(1815..>4853)
                     /locus_tag="VFV93_11930"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEX5166098.1"
                     /translation="TESTAEEAALTWLAELGYEIASGPELAPHERDDYKQVVLVGRLR
                     AAIGRINPGVPGEAREQALAEVLRAESQDLVGNNRRLHRLLVDGVPVEYRRADGSVVG
                     DRVMLVAWDEPGANDWLAVNQFTVVEKTNRRPDVVVFVNGLPLAVIELKDPTDPNATV
                     RKAFNQLQTYKRDIPSLFEPNALLVASDGLEARVGSLTADWERFMPWRTVSGDDLAPR
                     GSNELKVLLAGIFEHRRFLSLVRSGIVFEDDGGRTVKKLAAYHQFHAVEKAVAATLDA
                     ASERGDRRVGVVWHTQGSGKSLSMVFYAGRIIRHPAMENPTIVVLTDRNDLDDQLFGT
                     FARCADILGQDPRRAEARADLRGLLQVASGGVVFTTIQKFQPEEKGDSYPTLSERRNL
                     VVIADEAHRSQYDFIDGYARHLRDALPNASFIAFTGTPIETADKSTQAVFGDYIDVYD
                     IQQAVEDGATVRIFYESRLAKLALAESERPTIDAEFEEVTEGEETAGRERLKTRWARM
                     EAMVGAEKRVHLIAQDIVEHFEQRRQAIEGKAMIVAMSRRIAVDLYNALVALRPEWHS
                     DDDTSGVLKVVMTGSASDPQPFQPHIRSKARREALAERFKDPDDELRVVIVRDMWLTG
                     FDAPSLHTMYVDKPMQGHGLMQAIARVNRVFRDKPGGLIVDYLGIADQLRKALAAYTE
                     SDRRFTAIDQNEAVAILLEKLDIVRAMFHGFDYAPFLTGNAAAQMELLPKAMEHILTL
                     DDGKKRYLQAVTELSQAFALSVPHEEALAARDEVGFFQAVRSSIAKTTVSETREQEEV
                     EHAIRQIVSRAVAADEVVDIFAAAGLQRPDISILSDAFLAEVQGLPQKNLALEALRKL
                     LNDEITVRARRNVVEARSFREMLERAIQRYHNRGVETAEIVQELIELAKEMRAAAQRG
                     EQLGLNDEELAFYDALAESDSASAVLGDAQLRTIARELVETVRRNVTVDWTLRDSAQA
                     NLRRMVKRVLRRHGYPPDQQESATITVLKQAELFGNEWAA"
BASE COUNT          755 a         1710 c         1527 g          861 t
ORIGIN      
        1 cgcagcctcg cccttcgtca ttcgtccgga acagggcgag gctgcggcca tcgccctcac
       61 gcgggattcg tgctaccgcg ctatcagcac ggaatcgtca ggctctccag atcgcgggga
      121 taatacgtca gcgactcgat gcccgtctcg gtcacgacga cggtgtccga atggcgaaag
      181 cccgcgaagc ccgggacgta gatccccggc tcgaccgtga agaccattcc gacctcgatg
      241 acgcgctcat cgccgatatc gaggaacggc gcctcgtgga tgagcatcga cttggcgtgg
      301 ccggtgtggt gccgccagtt ctcctggatg ccatgctcct tgaagtagcg catcaactcg
      361 cggtcgacgg ccgcgcaggt gatccccggc ttgatcgtgt cgagcgcgag cgtttgagca
      421 cccagcatca acccgaacca gcgctcctgc tctttggacg gctcgcccat caccatcgtc
      481 cgctccagct cgctgccgta gccccagacg gccgcgccgg cgcctgtcac cagtacatcg
      541 cccttctgga gcttcacgtt gctggaaatg gcgtgcggca gcgccgagtc cttgccgacc
      601 tgcccccgga aggtggccat cgccgggaag cccgatgtca tcggccggta ctcgctgccg
      661 agcgcctcca tcatctccaa gctggcctcc aacgaggccc gcagcgcgat ctccgtctcg
      721 ccggctccga cggcgcagcc ttgctgcagg tactcgtggg ccaggttgcc ccacttgcac
      781 gactcgcgga tcagcgtcag ctcctcctcg gagttgatca tctgcatgta ctcgatctcg
      841 tccatgacgt tgacgatctc gacctccggc agcagctccg acaccttcgg gccgcgatag
      901 ccgtagacct ggccgtagcc gtccgaatcg accgccagct tgccgctgcc cagccccagc
      961 ccaaccagca tgtccttcag gaactcaagc ggatgccgct cgcctggata ctcggggtag
     1021 acgacgacct cgtcgacgat cgcgtactcc tgcgcgtgct cggtctccag cttcggcacc
     1081 agcagctcgc tacgcgtcgg cgtcagcacg taggccatcg gccgctcggt ctggatgaac
     1141 gtgaagcggg agaagtagga catcttgttc gggttgaaca acaccaaccc accgatcccc
     1201 cgctcgccca gcgacgccag gatcgcctcc cgccgccgtg catactcacc gtcgctcagt
     1261 cgaatcgttg ccaatcaatc cctccctcag cactcggatc ggctcgctac gccgcgataa
     1321 acgttctcac ctcgacctgg ataccggcct tggcggacgc atctgcgctc cgtagcaatt
     1381 gttccgcgac tgcttcgtcg acgtactctt cgccgcacgt gtcgcagacg cgcgcaggca
     1441 catcgcggaa gacaatcgtc atcgctccac gtgtaagcgt caccgtcgca gtcccgtcca
     1501 ccgtctcgcc gtgtttgcag atgacacagt tcatggcgtc ttcctcgtcc tgagatcagc
     1561 actccaccga ttgcgatcag gttcatagac cgtgacgacg atcgtttcat atgccaacgc
     1621 gttctccgca acgacaacgt gcaacggtcg aaggtctatc catcccaaca ccagccggct
     1681 cggatatggc caactcgtcg gataatcctc aattgtctca ccgcgttcaa ccacagcgcg
     1741 cacatcagca acactgactc cccgctcgtg catccgctgc aacgcatgcg tcctgaagac
     1801 gagccgattg gccatcaggc ggcccactcg ttgccgaaga gttcggcctg cttgaggacg
     1861 gtgatcgtag ccgactcctg ctggtcgggc gggtagccgt ggcggcgcag gacgcgcttg
     1921 accatccggc gcaggttggc ctgggcgctg tcgcgcaggg tccagtcgac ggtgacgttg
     1981 cggcggacgg tctcgacgag ttcgcgggcg atcgtccgca gttgggcgtc gccgaggacg
     2041 gcggacgcgc tgtcgctctc ggccagcgcg tcgtagaagg ccagctcctc gtcgttgagg
     2101 ccgagctgtt cgccgcgctg ggcggcggcg cgcatctcct tggccagctc gatgagttcc
     2161 tggacgatct cggccgtctc gacgccgcga ttgtggtagc gctggattgc gcgttccagc
     2221 atctcacgga acgagcgcgc ttcgacgacg ttgcggcggg cgcggacggt gatctcgtcg
     2281 ttgagcagct tgcgcagcgc ttcgagggcg aggttcttct gcggcagccc ctggacctcg
     2341 gccagaaagg cgtcggagag gatcgagata tccggccgct ggagaccggc cgccgcgaag
     2401 atgtcgacga cctcgtcggc ggccaccgcc cgcgagacga tctggcggat ggcgtgctcg
     2461 acctcctcct gctcgcgcgt ctcgctgacg gtcgtcttgg cgatgctgga acggacggcc
     2521 tggaagaagc cgacctcgtc gcgggcggcc agcgcctcct cgtgcggcac cgagagcgcg
     2581 aacgcctgcg atagctcggt gacggcttgc aggtagcgct tcttgccgtc gtccagcgtc
     2641 aggatgtgct ccatcgcctt cggcagcagc tccatctggg cggcagcgtt gccggtcagg
     2701 aacggcgcgt agtcgaagcc gtggaacatg gcgcggacga tgtcgagctt ctccagcagg
     2761 atcgcgaccg cctcgttctg gtcgatggcg gtgaagcggc ggtcgctctc ggtgtaggcc
     2821 gccagcgcct tgcggagctg gtcggcgatg ccgaggtaat cgacgatcag gccgcccggc
     2881 ttgtcgcgga agacgcggtt gacccgcgcg atggcctgca tcagcccgtg cccctgcatc
     2941 ggcttgtcga cgtacatcgt gtgcaggctc ggcgcgtcga agccggtcag ccacatgtcg
     3001 cggacgatca ccacccgcag ctcgtcgtct gggtccttga aacgctcggc cagcgcctcg
     3061 cgacgggcct tgctgcggat gtgcggctgg aacggctgcg ggtcggaggc agagccggtc
     3121 atgacgacct tgaggacgcc gctcgtgtca tcgtccgagt gccactcggg ccggagcgcg
     3181 accagcgcgt tgtagagatc gacggcgatc cggcggctca tcgcgacgat catcgccttg
     3241 ccctcgatcg cctgccggcg ctgctcgaag tgctcgacga tgtcctgggc gatcaggtgc
     3301 acccgcttct cggcgccgac catcgcctcc atccgcgccc agcgggtctt cagccgctcg
     3361 cggccggccg tctcctcgcc ctcggtcact tcctcgaact cggcgtcgat cgtcggccgc
     3421 tcggactcgg ccagcgccag cttcgccagc cggctctcgt agaagatgcg cacggtcgcg
     3481 ccgtcctcga ccgcctgctg gatgtcgtag acatcgatgt agtcgccaaa gacggcctgc
     3541 gtgctcttgt cggccgtctc gatcggcgtc ccggtgaagg cgatgaacga ggcgttcggc
     3601 agcgcgtcgc gcaggtggcg ggcgtagccg tcgatgaagt cgtactggct gcggtgcgcc
     3661 tcgtcggcga tgacgacgag gttgcgccgc tccgagagcg tcgggtagct gtcgcccttc
     3721 tcctccggct ggaacttctg gatcgtcgtg aagacgacgc cgcccgacgc gacctgcaac
     3781 aacccgcgca ggtcggcgcg cgcctcggcc ctgcgcgggt cctgacccag gatgtccgcg
     3841 cagcgcgcga acgtcccgaa gagctggtcg tcgaggtcgt tgcggtcggt caggacgacg
     3901 atcgtcgggt tctccatcgc ggggtggcgg atgatccgcc cggcgtagaa gaccatcgag
     3961 agactcttgc cgctgccctg cgtgtgccag acgacaccga cgcgccggtc gccccgctcc
     4021 gatgccgcgt cgagcgtcgc cgcgaccgcc ttctcgacgg catggaactg gtgataggcg
     4081 gccagcttct tgaccgtccg cccgccgtcg tcctcgaaga cgatccccga ccgcaccagc
     4141 gacaggaagc gccgatgctc gaagatgccg gccagcagca ccttcaactc gttgctgccg
     4201 cgcggcgcga gatcgtcgcc ggagaccgtc ctccacggca tgaagcgctc ccaatcggcc
     4261 gtcagcgagc cgacgcgcgc ctccagtccg tccgacgcga ccagcagcgc gttcggctcg
     4321 aacaggcttg ggatgtcgcg cttgtaggtc tggagctggt tgaacgcctt ccggacagtc
     4381 gcgttcgggt cggtcgggtc cttcagctcg atgaccgcca gcggcagccc gttgacgaag
     4441 acgacgacat ccggccgccg gttggtcttc tcgacgacag tgaactggtt gacggccagc
     4501 cagtcgttcg cccccggctc gtcccaggcg accagcatga cccggtcgcc aacgacgctg
     4561 ccatccgccc gccggtactc gaccggcacg ccatcgacca gcagccggtg caaccgccgg
     4621 ttgttgccga ccagatcctg actctcggcc cgcaacactt ccgccagcgc ctgctcgcgc
     4681 gcctcccccg gcacccccgg attgatccgc ccaatcgccg cccgcaaccg ccccaccagc
     4741 acgacctgct tgtagtcgtc ccgctcgtgt ggcgccaact ccggcccaga cgcgatctca
     4801 tacccaagct cagcgagcca ggtgagcgcg gcttcttcag cggtggattc ggt
//
LOCUS       DASUGJ010000528         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1334430, whole genome shotgun sequence.
ACCESSION   DASUGJ010000528 DASUGJ010000000
VERSION     DASUGJ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334430"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            387..1100
                     /locus_tag="VFV93_11935"
     CDS             387..1100
                     /locus_tag="VFV93_11935"
                     /inference="COORDINATES: protein motif:HMM:NF006093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166099.1"
                     /translation="MGDRPGGELSDDAVAALHVASTATITTQLMKRGFRNTFLAGLVP
                     TRPEMRMIGYAFTLRYVPMREDLDLVDIDNTTSVQRQAVEMVGPGDVLMIDARNDVGA
                     ATLGNILAERMRVRGAAGIVTDGALRDTSAYREIDLPTYVRATHQTQSTARHHPADLN
                     VPIGCAGVLVMPGDVVVGDADGVVVIPRAIADEVALAAAEQERREDFILEKIRAGASI
                     IGTYPPDEQTLAEYHARKS"
     gene            1200..1604
                     /locus_tag="VFV93_11940"
     CDS             1200..1604
                     /locus_tag="VFV93_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5166100.1"
                     /translation="MIRHLAGVGEIVEDVAAAVAFYRDVLGLEVEHDGGDYADVKLAG
                     IAHYGLWDRRAAAGAIYGDASQVDKVPLGFAVGFEVDAVEEATVTAGQRGLTFIQQPK
                     TEPWGQVTSRFILPSGLVGEFSETPWARQLGE"
     gene            1814..2347
                     /locus_tag="VFV93_11945"
     CDS             1814..2347
                     /locus_tag="VFV93_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166101.1"
                     /translation="MTDRLTIRPAWSILRVAVIGVLMLSLVAACGSGDDDDDDSGSAP
                     TATTAASSNSDETPTESGMSMGSVTTDDMAEAMAVSSCLEDGTTGDIVTDLRDGDTGS
                     AEDLYRGCLSDALPAAMVSQLDPIIEKAGDCGSEAAADLSDDDVAAIEDGDDAKIQQL
                     TNDTLECLSADLGIDLQ"
     gene            2430..>2862
                     /locus_tag="VFV93_11950"
     CDS             2430..>2862
                     /locus_tag="VFV93_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166102.1"
                     /translation="MTERTAHRRRRPIGRIGLLLALLPLLLFGCSTSGGESSTPTPTA
                     TATSSPTPEPTSTPTATPSPTAFVPPTVAVEDDDSIQACMERNLTPELLISLSKDDTS
                     LTEDIMRTCLETTIPSPLMFLLNPIIDDASECALDVSKTLSN"
BASE COUNT          526 a          924 c          945 g          467 t
ORIGIN      
        1 tcgtcgccag caactggacg atgtccggct ggtcatcaac gatcaggatc ggcgcgtcgc
       61 tgacggcaat ccgcgtcagg cggctcgtca tcccgcgcga gtgatctgct ggcgactggg
      121 attgctgctc gtcggacatt aggtctctct cggcgttccg gcgcgttggg ccatccgcga
      181 ttggatcgac tatgtcatca tgggaaccac ggccggcggg accttcgtcg tgccgcgccc
      241 ggttcggcgg cgattgaagt actctgcgat ctcatcaatt ccgcacccgg cgcggcagcg
      301 aacgaggtgc atatgctgag tgtcgcacag gtattcaggg cggatttgac gacagaagta
      361 ccaaaacagg ggaggacgat ggagctatgg gtgaccggcc aggcggcgag ctttccgatg
      421 atgcagtcgc ggcgctgcat gtcgcgagta ccgcaacgat cacgacccaa ctgatgaagc
      481 gcggctttcg caacacattt cttgcaggac tggtaccaac gcggccggag atgcggatga
      541 tcggttacgc cttcacgcta cgctacgtgc cgatgcgaga ggatctcgac ctggtcgata
      601 tcgacaacac gaccagcgtc cagcgccagg cggtcgagat ggtcgggcca ggcgacgtgc
      661 tgatgatcga cgcgcgcaac gacgtcgggg cggccacgct cggtaatatc ctcgccgaac
      721 ggatgcgcgt ccggggcgcg gccgggatcg tcaccgacgg cgcgctacgg gacacgtcgg
      781 cctaccgtga gatcgacctg ccaacctacg tccgtgcgac ccaccagacc cagagcacgg
      841 cccgccacca tccggccgac ctgaacgtgc cgatcggctg cgccggtgtg ctcgtcatgc
      901 ccggcgatgt tgtcgttggc gatgccgacg gcgtggtcgt catcccgcgc gccatcgccg
      961 acgaggtcgc gctggccgcc gccgagcagg agcggcgcga agacttcatc cttgagaaga
     1021 tccgcgccgg cgccagcatc atcggcacct acccgccgga cgagcagaca ctggccgagt
     1081 accacgcgcg gaagtcataa gatcgctcag ccgcccagtc gcaagcgctt gacatcgaac
     1141 atatgttcgc ctacgctggc cgtccgttca tcgttcgtca tggacacgag gaggttcgga
     1201 tgatcaggca tctggcaggg gtcggtgaga tagttgagga cgtcgcagcg gccgttgcgt
     1261 tctatcgcga cgtgctgggg ctggaggtcg agcacgacgg cggcgactac gccgacgtca
     1321 agctggccgg catcgcccac tacggactct gggaccggcg cgccgccgcc ggtgcgatct
     1381 acggcgacgc gagccaggtt gacaaggtgc cgctcggctt cgcagtcggc ttcgaggtcg
     1441 acgccgtcga ggaggcgacc gtcacggccg gtcagcgcgg cctgacgttc atccagcagc
     1501 caaagaccga gccctggggc caggtcacct cccgcttcat cctgcccagc ggtctggtcg
     1561 gcgaattctc agaaaccccc tgggcgaggc aactgggcga gtagcgaccg ctccctttca
     1621 tatgccaaca aggacggccg gcacgaggcc ggccgtcctt gttggcgatg tcgccactcc
     1681 tgtttccgca catctgtttc gtgattcgcg gctgttgtcc cgtggaacgc cagatgcgtg
     1741 ggtagtgagg ccggcgccca ggtgacgccg gcctgaactc gactgagggg cgacatcgaa
     1801 agggtgaacg atcgtgactg accgactgac aatccgcccg gcctggagca tcctccgggt
     1861 cgcggtgatc ggtgtgttga tgctctcgct ggtcgcggcc tgcgggagcg gcgacgacga
     1921 cgatgacgat tccggcagcg cgccgaccgc aacgacggcc gccagcagca actcggacga
     1981 gacgccgact gagtccggca tgagcatggg ttcagtgacg accgatgata tggcggaggc
     2041 gatggcggtc agctcctgcc tggaggacgg cacgaccggc gatatcgtga ccgatctgcg
     2101 cgacggtgat accggcagcg ccgaagatct gtatcgcggc tgcctcagcg atgccctgcc
     2161 ggcggcgatg gtcagccaac tcgatccgat cattgagaag gccggcgact gcggcagcga
     2221 agcggctgcc gacctgagcg acgacgacgt cgcagcgatc gaggacggcg acgacgcgaa
     2281 gatccagcag ctgacgaacg atacattgga atgcctctcg gccgatctcg gaatcgacct
     2341 gcaataggtg ggctgacgac gcggcggcgc gcgatccttt cgccgccgcg tcgtcagcgg
     2401 aaccgctatc cggccgaagg gaggacagta tgacggagcg aaccgcccac agacgacgcc
     2461 ggccaattgg acggatcggc ctgttgctgg cgctgctccc gctgctgttg ttcggctgct
     2521 cgaccagcgg tggggagtcc tcaacgccga ctccaacggc aaccgccacc agctccccga
     2581 caccggaacc cacctcgaca ccgacggcga caccgtcgcc aaccgccttc gtcccgccaa
     2641 cggtggccgt cgaggacgac gactcgattc aggcgtgcat ggagcggaac ctgacgccgg
     2701 agctgttgat cagcctgtcg aaggacgaca cctcgctgac cgaagacatc atgcggacct
     2761 gtctggagac gacgatccca tcgccgctca tgttcctgct gaacccgatc atcgacgacg
     2821 ccagcgagtg tgcgctggat gtctcaaaga cgctctccaa cg
//
LOCUS       DASUGJ010000529         6010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_918409, whole genome shotgun sequence.
ACCESSION   DASUGJ010000529 DASUGJ010000000
VERSION     DASUGJ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918409"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..2277)
                     /locus_tag="VFV93_11955"
     CDS             complement(130..2277)
                     /locus_tag="VFV93_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5166103.1"
                     /translation="MRAIEPVTSALVRNPADGVRVRYEVFGSDDARRAIVFSPAGSYI
                     HGRVWKGQAPWFVQRGYRVVTWDGRGSGGSDRPATGYSPDHFAGDLLAVMDAAGIERA
                     ALVGMTWAIRWMSRVAARHPERVSHLISVSSFPTHFDTSRHEAMLGYFLSDPDSGGEL
                     DWKAVHMRQDYRALNEQTACDDFPEPHSTRQIEDFVSWTMEASPEALVTSCLELFESD
                     PATFHRRVACPTLLIHGSADVSVGPAFGHALQAEIPGAELLVFEGSGHVPIARDAVRA
                     NLAIDAFIERSAPQVAPQVRRWQRAMARPRQALFISSPIGLGHVQRDLAIARELRRLV
                     PDLQIDWLAQPPVSGVVEQAGETIHPLSSALASESAHWEAAAGDHQLHCFNAFREMDE
                     ILLANFMVFLDAVRETPYDLWIGDEAWEVDHFLHENPELKTAPFVFMTDFLGWLPVDD
                     TPGSREAFLTADYNAEMLEHVERFPYIRDRAIYFGEYDDLVPDRFGPDLPLIRDWARE
                     HFTAVGYVVPFDPADYADTSVARERLGYDPQQPLLIAAVGGTAVGRHLLDKVVASWPM
                     IRRERPTLRGVVVTGPRIDPATIPEQPGLEVRPYVHNLYEHLAVADLGVVQGGLSTTM
                     ELTVNRRPFLYFPLRDHCEQVLHVTHRLDRYHAGVRLDYDATTPESLAAVALATLGSD
                     TSDYRPHEPGAAGRAAALIAEVIGIRGSGFGVV"
     gene            complement(2264..3169)
                     /locus_tag="VFV93_11960"
     CDS             complement(2264..3169)
                     /locus_tag="VFV93_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5166104.1"
                     /translation="MRAIEPTSAGQLTIEGFSVGYEAFGDPANPAVLLLPSWQIVHSR
                     AWKMQVPFLARSRRVVTFDPPGNGGGERTTDPAAFEYERIVCQAIGVLDALDIAQAAV
                     LGYGRGCDYGLLLAATAPERVERLMLIGNGVTRAGWQPKPDPGFWDRRPTYEGWQKRN
                     AHYFLEQYDDWLTFFVGELHPEPHSTKATEDMIGWGRETSPEMLVRTVPNGDLLPRLP
                     AAEAIASIRCPVMLLHGGDDRCDPIEASYDLLDARPDWQMVVMEDCGLDVLGRHSVQV
                     NREVEAFLHRASPAGQRTRTSDARD"
     gene            complement(3173..4057)
                     /locus_tag="VFV93_11965"
     CDS             complement(3173..4057)
                     /locus_tag="VFV93_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5166105.1"
                     /translation="MRAMQPVDSGIVTLEGFQVAYESFGNPAWPAVLLLPPWQIVHSR
                     IWKMQVPYLARSFRVVTFDPPGNGNSERTADPTAYEFECVARIGLGLLDQLGIQRSAL
                     AGYSRSCAYALWLAATAPERVERLVLIANGVTPDDWGSGASPEFFERRETYDGWQRYN
                     AHYWREHYDEWLEFFFTQLFPEPHSTKGIEDGIGWGHETTAETLIHSTTSPHLRPAMP
                     PRDAIRAIRCPVLLLHGDCDDISPIETSRTLALARPDWQMITLAGCSHGAVVRDPVRV
                     NLFIHDFLAASTWHPEGR"
     gene            complement(4091..>6010)
                     /locus_tag="VFV93_11970"
     CDS             complement(4091..>6010)
                     /locus_tag="VFV93_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5166106.1"
                     /translation="LELSFGNSVDERTSLLHWLQERELLLVLDNAEHLLDGVDLLSEI
                     LARAPDVQLLVTSRARLDLHGEWVVEVGGLSLPGALPGAAISGAEQLFMQRSGQVVAG
                     FAPVAAERAAIAALCRLVEGMPLALEIASGWLPVLSCAEIVSEVTRSLGFLETTQRDV
                     EPRHRSVHAVFDRSWELLGEQEQRAFRRLAVFRGSFRRDAAERITGASLPLLSALVSR
                     SLLRRTSAGRYEMHELLRQYAGEQLAQQPDEAAVVRDRHCLYYADLISRRTVDLRGAG
                     QAEVLADLEAEIENLRAALIAATEDARPGVIADAADGFWLFCEITGRHRDYQQLLRAA
                     VESVDRSDRVPAAPDADALALGKILVLAGSTFARTGEYEHGDALVTRGVSMLRCLDAA
                     EEIGLGLNFQAMYAHRRQDFEAERALLRESIERSELAGDRWVMAYSLNDLGLAMLDLG
                     DADEARRLQLESLAIFRDIGDKRGAAFALHNLGVVARHQGDAGAATFLNEALAIRHEI
                     RHLWGIAETLIELGVVARESGDTAQAVDHFRDGLRIAEEIHALPAILRALVEIAGVLA
                     FAGERQRADQLLADILRHPGADIQIRSRAGELLGAAGDAVSANGAIAPGQADDIIDAQ
                     VTSFLRNDPTWASVT"
BASE COUNT         1024 a         2031 c         2026 g          929 t
ORIGIN      
        1 catctgtgcc gtcagccctt cgcgattcgt gccaatcgat ggtgacagcc tatgcccgtg
       61 cgggtgctgc gtcaagcgtg attgggattt gggatttggg attcgggggc ttcgagtagc
      121 gcaccgccgt cagacaaccc cgaatcccga gccccgaatc ccgatcacct ccgcgatcag
      181 cgccgccgcc ctgccggccg cgccgggttc gtgcggccga tagtcgcttg tgtcgctgcc
      241 gagggtcgcg agggcgacag ccgccagtga ttccggggtg gtcgcgtcgt agtccagccg
      301 gacgccggcg tggtagcggt cgaggcggtg ggtgacgtgc agcacctgct cgcagtggtc
      361 gcgcagcggg aagtagagga acggcctccg gttgacggtc agctccatcg tcgtgctcag
      421 cccgccctgg acgaccccga ggtcggccac ggccaggtgc tcgtagaggt tgtggacgta
      481 gggccggacc tccagtcccg gctgctcggg gatcgttgcc ggatcgatcc gtggtccggt
      541 gacgacgacg ccgcgcagcg tgggccgctc gcgccggatc atcggccagc tggcgacgac
      601 cttgtccagc aggtgcctgc caaccgccgt cccgccgacg gccgcgatca gcagcggttg
      661 ctgcggatcg tagccgagcc gctcgcgggc gacgcttgtg tcggcgtagt cagccggatc
      721 gaacgggacg acgtagccaa ccgccgtgaa gtgctcccgc gcccagtcgc ggatcagcgg
      781 caggtccggt ccgaagcgat cggggacgag gtcgtcgtac tcgccgaagt agatggcccg
      841 atcgcggata tacgggaagc gctcgacatg ctccagcatc tcggcgttgt aatcagccgt
      901 caggaacgcc tcgcgcgagc cgggcgtgtc gtcaaccggc agccagccga ggaagtcggt
      961 catgaagacg aacggggccg tcttcagctc cgggttctcg tggaggaagt ggtccacctc
     1021 ccaggcttcg tcgccgatcc agagatcgta gggcgtctcg cggacggcat cgaggaagac
     1081 catgaagttc gccagcagga tctcgtccat ctcgcggaat gcgttgaagc aatgcagctg
     1141 gtggtcgccg gccgccgcct cccagtgggc cgattcgctg gccagcgcgc tactcaacgg
     1201 gtggatcgtc tcgccggcct gctccacgac accgctcacc ggcggctggg ccagccagtc
     1261 gatctgcagg tccggcacga gccggcgcaa ctcgcgggca atcgccagat cgcgctggac
     1321 atgaccgagg ccgatcgggc tggagatgaa cagcgcctgc cgcggtcggg ccatcgcccg
     1381 ctgccagcgt ctgacctgcg gcgcgacctg cggcgcggag cgttcaatga acgcatcgat
     1441 cgccagattc gcgcgaacgg cgtcccgcgc aatcgggaca tgcccgctac cctcgaagac
     1501 gagcagctcg gcgccgggaa tctcggcctg caatgcgtgg ccgaacgcgg gaccgacgga
     1561 gacatcggcg ctcccgtgga tgagcagcgt cgggcaggcc accctgcgat ggaacgtggc
     1621 cgggtcggac tcgaacagct ccaggcagga ggtgaccagc gcctcgggcg atgcctccat
     1681 cgtccagctg acgaagtcct cgatctggcg ggtcgagtgc ggttcgggga agtcgtcgca
     1741 tgccgtttgc tcgttcagtg cgcggtagtc ctgccgcatg tggaccgcct tccagtcgag
     1801 ttccccgccg gaatcaggat cggacagaaa atagccgagc atcgcttcat gccgggaagt
     1861 gtcgaagtgc gtcgggaagg agctgacgct gatcaggtga ctgacccgtt cgggatgccg
     1921 ggcggcgacg cggctcatcc agcggatggc ccaggtcatg ccaaccagcg cggcacgctc
     1981 gattccggcc gcatccatga ccgccagcag atcgccggcg aagtggtcgg gcgagtagcc
     2041 ggtcgccggc cgatccgacc cgccgctgcc gcgcccgtcc caggtcacaa cccggtagcc
     2101 acgctggacg aaccacggcg cctgcccctt ccagacgcgg ccgtggatgt acgaaccggc
     2161 cggcgagaag acgatcgctc gcctggcgtc gtcgctgccg aacacttcgt agcggacccg
     2221 gacaccatca gccgggttgc gcaccagcgc gctggtgact ggttcaatcg cgcgcatcgc
     2281 ttgtcctcgt gcgctgaccg gcgggcgatg ctcgatgtag aaacgcttcg acctcgcggt
     2341 tgacctggac agaatgacgc ccgaggacat cgagaccaca gtcttccatc acgaccatct
     2401 gccagtcggg gcgcgcgtcg agcagatcgt agctggcctc gatcggatcg cagcggtcgt
     2461 cgccgccatg cagcagcatg acgggacagc gaatgctcgc gatcgcctca gcggccggca
     2521 gacgcggcag tagatcgccg ttcgggacgg tccggaccag catctccggc gatgtctcgc
     2581 gcccccagcc gatcatatcc tcggtcgctt tggtcgagtg cggttctgga tgcagctcac
     2641 cgacgaagaa cgtgagccag tcgtcatact gctccaggaa gtagtgcgcg ttgcgcttct
     2701 gccagccctc gtaggtcggc cggcgatccc agaagccggg atcgggcttc ggttgccacc
     2761 ccgcccgtgt gacgccgttg ccgatcagca tcaaccgctc aactcgctcc ggcgcggtcg
     2821 cggccagcag caggccgtag tcgcagccac gcccatagcc gagaaccgcc gcctgcgcga
     2881 tgtcgagggc gtcgaggacg ccgatagcct ggcagacgat ccgttcatac tcaaacgcgg
     2941 ccgggtcagt cgtccgttcg ccgccaccgt tgccgggtgg gtcgaacgtg acgacccggc
     3001 gggagcgcgc cagaaacgga acctgcatct tccaggcgcg gctgtggacg atctgccacg
     3061 acggcaagag cagcacggcc ggattggccg gatcgccgaa tgcttcgtac ccgacactga
     3121 acccttcgat ggtgagctga ccggcagagg ttggctcaat cgctcgcatt ggctaccgcc
     3181 cctcagggtg ccacgttgac gccgccagga agtcgtggat gaacaggttg acacggaccg
     3241 ggtcacggac gacggcaccg tggctgcagc cggccagtgt gatcatctgc cagtcgggcc
     3301 gggccaacgc cagtgtccgg ctggtctcga tcggcgagat gtcatcacaa tcgccgtgca
     3361 gcagcaggac cggacagcgg atcgcacgga tcgcgtctcg gggcggcatg gccgggcgca
     3421 ggtgcgggct ggtcgtcgag tggatcagcg tctcggccgt cgtctcgtgg ccccagccga
     3481 tgccatcctc gattcctttc gtcgagtgag gctcggggaa cagctgcgtg aagaagaact
     3541 caagccactc gtcgtagtgc tcgcgccagt agtgcgcgtt gtagcgctgc cagccgtcat
     3601 acgtctcacg ccgctcgaag aactcggggc tggcgcccga gccccagtcg tccggcgtga
     3661 caccattcgc gatcaggacg agccgctcga cccgttccgg cgcggtcgcg gccagccaga
     3721 gcgcgtaggc acagctgcgt gagtagccgg ccagtgcaga gcgctggatg ccgagttgat
     3781 cgagcagacc gagcccgatc cgcgcgacgc attcgaactc gtacgcagtc ggatcggccg
     3841 tccgctcgct gttgccgttg ccgggcggat cgaaagtcac aacccggaac gagcgcgcca
     3901 ggtacggaac ctgcatcttc cagatccggc tgtggacgat ctgccagggc ggcagcagca
     3961 ggacagccgg ccaggcaggg ttgccgaacg attcataggc cacctgaaac ccttcaaggg
     4021 tcacgatgcc gctatcgact ggctgcatgg cacgcatgga gcggttgctt cctgtctcta
     4081 gccctgctgg ctacgtcaca gatgcccagg ttggatcatt gcgcaggaac gacgtgacct
     4141 gcgcgtcgat gatgtcatcc gcctgtccgg gcgcgatcgc gccgttcgcg gaaacggcat
     4201 ctccggctgc gcccagcagt tcgccggctc gactgcgaat ctgaatgtcg gcgccggggt
     4261 gtcgcagaat gtcggccagc aactggtccg cgcgctgccg ttcccctgca aatgccaaga
     4321 ccccggcaat ctccaccagc gcacgcagga tggccggcaa cgcgtgaatc tcctccgcga
     4381 tccgcaggcc atcgcggaag tgatcgacgg cctgcgcagt gtcgccggat tcacgcgcga
     4441 cgacaccaag ctcgatcagc gtttcggcga tcccccagag atggcggatc tcgtgacgga
     4501 tcgcgagcgc ttcgttcagg aacgtcgccg cgccggcatc tccctggtgg cgagcaacga
     4561 cgccgaggtt gtgcaatgcg aaggcagccc cgcgtttgtc gccgatgtcg cgaaagatcg
     4621 ccaggctctc cagctgcaag cgtcgcgcct catcggcgtc accgaggtcg agcatcgcca
     4681 ggccgaggtc gttgagcgag taggccatga cccagcgatc gccggccagc tccgagcgct
     4741 cgatgctctc gcgtagcagg gcgcgctcgg cctcgaagtc ctgccgccgg tgtgcataca
     4801 tggcctggaa gttcaggcca agcccgatct cctcagctgc gtccaggcag cggagcatcg
     4861 agacaccccg cgtgacaagc gcgtcaccgt gctcgtattc gccggttcgg gcgaaggttg
     4921 aaccggccag gaccaggatc ttcccgagcg ccagcgcgtc ggcatcgggc gcggccggga
     4981 cgcggtcgct gcgatcgacg gactcgacgg cggcacgcag gagctgctgg taatcgcggt
     5041 gccggccggt aatctcgcag aagagccaga acccatcggc cgcatcggcg ataacgccgg
     5101 ggcgggcatc ttcagtggcc gcgatcagcg cggcgcgcag gttctcaatc tctgcctcca
     5161 ggtcggccag cacctcggcc tgacctgcgc cacgcagatc gactgtccgg cgggagatca
     5221 gatcggcgta gtacaggcaa tgccgatcgc gaacgaccgc tgcctcgtca ggctgctggg
     5281 ccaactgctc gccggcgtac tggcgcaaca gctcgtgcat ctcatagcgg ccggcgctcg
     5341 tccggcgcaa cagcgagcgg ctgaccagcg ccgacagcag cggcagcgac gcgccggtga
     5401 tccgctcggc cgcgtcgcgc cggaaactgc cacggaagac cgccagccgg cggaaggcgc
     5461 gctgctcctg ctcgccgagc aattcccagg aacgatcgaa gacggcgtgg acgctgcggt
     5521 gccgcggctc gacgtcgcgc tgcgtcgtct ccagaaagcc gagactgcgc gtcacctcgc
     5581 tgacgatctc ggcgcaggac agcaccggca accagccgga ggcgatttcc agcgccagcg
     5641 gcatcccctc gaccagccgg cagagggccg cgatcgccgc ccgctcggcc gcgacgggcg
     5701 cgaagccggc cacgacctgc ccagagcgct gcatgaagag ctgctcggcg ccggagatgg
     5761 ccgcgccggg caacgcgccg ggcagcgaca acccgccaac ctcgacgacc cactccccgt
     5821 gcaggtcgag ccgcgcccgc gaggtgacca gcagctggac atctggcgcc cgggcgagga
     5881 tctcgctgag cagatcgacg ccgtccagca ggtgttcagc gttgtcgagc accagcaaca
     5941 gttcccgctc ctggagccaa tgcagcagcg acgtccgctc gtcaacgcta ttgccgaacg
     6001 acaactcaag
//
LOCUS       DASUGJ010000530        10626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1473226, whole genome shotgun sequence.
ACCESSION   DASUGJ010000530 DASUGJ010000000
VERSION     DASUGJ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473226"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFV93_11975"
     CDS             complement(<1..777)
                     /locus_tag="VFV93_11975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5166107.1"
                     /translation="MQLGVVFPQTEIGSDPAVVREYAQTAEGSGLKHLLVYDHVMGAS
                     TANRPNWRGPYTSETPFHEVFVLFGYLAAATPGLELVTGVVILPQRQTALVAKQAAAI
                     DVLTGGKLRLGVGVGWNDVEYTVLNENFNNRGRRFEEQIEVLRALWTDEVIDFKGRYH
                     TIPEAGLNPMPVQRPIPLWIGGYADVVMERIGRLADGWFPGSQPGDVLEHSRSLVVAS
                     AEAAGRDPSAIGMEGRITLEPDAESEWIAQTEAWRAAGATH"
     gene            891..1226
                     /locus_tag="VFV93_11980"
     CDS             891..1226
                     /locus_tag="VFV93_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166108.1"
                     /translation="MAHQALAESRLRGLPVAEEYRVISDRLYRAGVRDLPPGVLIDWI
                     EQTVEEEYEHFLELRALGERFHTAIAAGDLAQAAWNLAADLDPQARQRLIDGLIRLNQ
                     LENNAAGGR"
     gene            complement(1251..1913)
                     /locus_tag="VFV93_11985"
     CDS             complement(1251..1913)
                     /locus_tag="VFV93_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166109.1"
                     /translation="MREPHELIQVVIVDDHPLFRDGVVNTLSAQPDIEVVGEGASADD
                     ALRLAQAFLPDTILLDISMPGGGLQAVGKIAASVPVTSIVMLTASEHEEDVTAALKAG
                     ARGYIVKGVAARELVNIVRSIHVGEVYVTPSLAATLLFELTGAGSRSSAGGKLLDELT
                     ERERQILERVAAGDSNKEIGSALFISEKTVKHHMTNILQKLQVRNRVEAAMLTHRESR
                     GD"
     gene            complement(1906..3351)
                     /locus_tag="VFV93_11990"
     CDS             complement(1906..3351)
                     /locus_tag="VFV93_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5166110.1"
                     /translation="MRSAIRALLQPLRRLSFAQRFMLASLVILVTGMFGVGTWVANRI
                     ESGVVHRTSATTALYVDSVVAPHLQGMATGSTISAAEQNELNHLFADTPLGEQIAAFK
                     VWGPGGLVVYSTEPSVIGKVYPVHPRLRESWDGAVTAGISDLDAEENEMERQSQARLL
                     EIYTPVRLRGTNDVIAVAEFYQVTDALDRELTSATRTSWLIVGGATLVMYLLLAGFVQ
                     RASNTIVRQRGELESQVDRLTDLLQQNEQLHDRVRRAAARTTALNERFLRRFSSELHD
                     GPAQDLSLALLELDNVIVSCGGLPDAASCTRQLDTIQQSVRRALQEIRTTSAGLMLPQ
                     LSKLPLADTVRHAVRAHERRSGTQVSVVLGPLPDDAPVATKIAIYRVIQESLTNGVRH
                     AGGIQQEVRVGVDDASFTVQVIDHGHGFDATRVLASDEHMGLVGMRERVESLGGMLVI
                     ESAPGRGTTVIAHLPVETVPAEDRAEELVHA"
     gene            3652..4047
                     /locus_tag="VFV93_11995"
     CDS             3652..4047
                     /locus_tag="VFV93_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166111.1"
                     /translation="MTRTQLRWMVPVLALIGLLVFSVSADAKGAETIVRLKGSSSYPG
                     VSGKAKYKVDGTQREFEVEIEDANRLIGKTLTVSVNGKTVGTMRVNSFGSAALERNTR
                     LRQTVPTITSGATVRITTSTGVLVASGRF"
     gene            4218..5960
                     /locus_tag="VFV93_12000"
     CDS             4218..5960
                     /locus_tag="VFV93_12000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5166112.1"
                     /translation="MAEKSTVSLSVERNVAVPMRDGTILYADVYRPAGPGPFPALLTR
                     SPYDKAVTGAAPFTVRAATAGYAVVIQDVRGRFESEGEFYAFVNERQDGYDTLEWLAS
                     QPWCDGKIGMFGGSYVGLTQWQAALSGHPALKAIVPNVTAANYHNGWTYQGGAFELQF
                     NLSWTLSVLAINTSLRANNGDINSPAFQAIMDASDNMTPEFEKLPLTGNEVFGEFARY
                     YDDWVNHPTYDDFWSGLDVSRGWESITAASFSMGGWHDIFLGGTIDNFAGMTAASGDG
                     RHRLMIGPWNHGGMRTGNPIGDENFGMRSTGAVIDEGGMHLRWYDRWLRDIDNGIEND
                     PPVKLFVMGSNRWREAQTWPLENTDWQEWFLHSGGTANTLNGDGALSREAPGGEPADS
                     YVYNPRNPVPTRGGGLCCNNVFSLGGAWDQREVEQREDVLVYTSAPMEKAREVTGPIK
                     VILYASSSATDTDFTAKLVDVGPCGYARNLTDGIIRARYRESMAEGTLITPGEVIEYE
                     IDLWATSNLFKEGHRIRVEISSSNFPRFDRNPNTGELPGRSTEMVSALQTIHHSTEYP
                     SRIVLPVINDPAGP"
     gene            5982..7637
                     /locus_tag="VFV93_12005"
     CDS             5982..7637
                     /locus_tag="VFV93_12005"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5166113.1"
                     /translation="MTLTLIRDRAPDVILTDARITTMDADLPEAEAVAIKDGRILAVG
                     PAGAIEELAGPATVVRRLGGRRLIPSINDTHNHLMSMGKVLSEVQLYDARSIDDIKER
                     VAERVASAPPGAWITGRGWDESLLRDGRFPNRHDLDDVAPNNPVVLDRVWNMLVANSA
                     ALHAADVGRETPDPPADQLYAGRIVRDEHGDPTGVFRDRAKRLIQDVIPLPTAADRES
                     YIRTACAEYNRMGITSAAEPGLFPEQLRAYQNVRRAGDLSIRVSLCLAGWGFGTASDD
                     ATVEQRIDDTGVYTGYGDDWLRFDTVKFMPDGGVGDRTALMFEHYEGEPDNTGQFVVS
                     ERDLFRNVAWVHERGWSIDCHACGDRMIEMVARAYAAAQSARPDATIRHRIHHAYLPT
                     PTALDLMREYRIPALATIPFIHNLGESFVTSLGEQRAARVMPLRTYLDAGVPLALGSD
                     APVTTHNPFVGIYSAVTRKTVYGRQLGAEECISREEALRAYTIDAAWVTFEDDSKGSI
                     APGKLADLAVLDRDILSVPEDEIPMTTSLLTLAGGRAIHDRLV"
     gene            7653..8534
                     /locus_tag="VFV93_12010"
     CDS             7653..8534
                     /locus_tag="VFV93_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5166114.1"
                     /translation="MALAYVRPRESTFDAKVLAWVDLASQRVFALDGTLAGLLRLSPD
                     ERSRQINALRQQADANLALDEVAFLAPVDEQEVWAAGVTYERSRDARMEESSVDDVYA
                     RVYDAERPEIFFKAPAWRCVGDFQPVAIRHDSTWDVPEPELALVIDSEGTIAGYTVGN
                     DVSSRSIEGENPLYLPQAKMFSGSCALGPWIVLANELHDPLALDIRLRIERDGQPHWS
                     GQTSTRSFHRSLDQLVECLYSALDFPAGAVLMTGTGIVPPSEFTLESGDTVSIEIDGI
                     GTLTNHVYRLPERSANG"
     gene            8527..8886
                     /locus_tag="VFV93_12015"
     CDS             8527..8886
                     /locus_tag="VFV93_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5166115.1"
                     /translation="MAERAQVIDWTGIEASEPFPGIVRQALVTATSTIVRYTYHPGCV
                     FPVHQHPEEQLTLVHSGEIEFEVADEPVILRAGQIAVIPGGVPHGARVTASEVVITDN
                     YIASANRTPLQFEGGQS"
     gene            8883..10553
                     /gene="ggt"
                     /locus_tag="VFV93_12020"
     CDS             8883..10553
                     /gene="ggt"
                     /locus_tag="VFV93_12020"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5166116.1"
                     /translation="MSRFVTSEWLMDKTGASSDGGMVVAKQEIAAMAGAQVLAEGGNA
                     VDAAVTTAWVMNVMEPYNCTIAGAGYLVYRAASGESWVVDFSNRAPSRATPEALASRT
                     TVAFAGPLAAAVPATVAGLATALERFGTISLARSLAPAIEVAAEGMPLNWLLTLRLVQ
                     ELPGIRANPKTAEIFLTNGDPGMAHGETVLRQTDLARTLRAIADDGIGAFYEGEIASK
                     IVAFVNDQGGILDAADFVRFQPTVVEPLRASYRDYTLLGVPLPSPGLTTIQCLRMLAG
                     DDLDAMGHNSADALHLLAEAFRLAFADRDAYLGDPEFSDPPVEALLSDDYISRRRAEI
                     DSRRAMEVVRPGDVGRVPLGAEGGGGGTTQFCAVDAAGNMVSLTQTLIGGLTGLGVAG
                     DTGVVMNCALQWFDPRPGAPNSVAPNKRPLSNMTPMIVERDGKPVLAVGAPGSRRITN
                     AVTQVTLNVLEHGLSAQAAISAPRIDCSLGHILADDRIDAATIADLRERGHRVDIVNE
                     FINSGGPSTGYRGNFSRPAAIHIDAEGIRHGGDYPFVEGMAVGVPRDA"
BASE COUNT         1905 a         3589 c         3387 g         1745 t
ORIGIN      
        1 gtgagttgcc ccagcggctc gccaggcctc ggtctgggcg atccattcgg attcggcgtc
       61 cggctccagc gtgatgcggc cttccatccc gatggccgac gggtcgcgac cggcggcttc
      121 ggcgctggcg acgaccaacg agcgggaatg ctccagcacg tcgccgggtt ggctaccggg
      181 gaaccagccg tcagccagtc gcccgatccg ctccatgacg acgtccgcgt agccgccgat
      241 ccagagcggg atcggccgct ggaccggcat cgggttcaag ccggcctcgg ggatcgtgtg
      301 gtagcggcct ttgaagtcga tcacctcgtc ggtccagagc gcgcgcagga cctcaatctg
      361 ttcctcgaag cggcggccac ggttgttgaa gttttcgttg aggacggtgt actcgacatc
      421 gttccagccg acaccaactc ccagccgcag cttgccgcct gtcaggacat cgatcgccgc
      481 cgcctgcttg gcgaccagtg ccgtttggcg ctggggcagg atgacgacgc cggtgacgag
      541 ttccagcccc ggcgtcgcgg ctgccaggta gccgaacagg acgaacactt cgtggaacgg
      601 cgtctcggag gtgtacgggc cgcgccagtt ggggcggttg gccgtgctgg cgcccatgac
      661 gtggtcatag accagcaggt gcttcaggcc ggagccttcg gcagtctgcg cgtactcacg
      721 gacgacagct ggatctgacc cgatctccgt ctgcggaaag accacaccaa gctgcatcgc
      781 gttcccagtc cctatctatt acgtcgcttg cggcaggcgg ctatactcga tacatgagtg
      841 tacgccgcaa tcgacattcg atcgcgacaa tccccgagga tggtctgcct gtggcacatc
      901 aggcgctggc tgagtcgcgt ctgcgcggcc tacccgtggc cgaggagtac cgggtgatct
      961 cggatcggct ctatcgcgcc ggtgttcgag acctgccgcc aggcgtcctg atcgactgga
     1021 ttgagcagac cgtcgaggaa gagtacgagc atttccttga actccgcgcg cttggcgagc
     1081 gctttcatac ggcaatcgct gccggcgatc tcgcccaggc agcctggaac ctcgcggccg
     1141 accttgatcc gcaggcgcga caacgcctga tcgacgggtt gatccggctc aatcagcttg
     1201 agaacaacgc agccggagga cgttgaagac acaacggacg gcgtgacgcg tcaatccccc
     1261 cgcgattccc tgtgagtcag catggcagcc tcgactcggt tgcgaacctg cagcttctgc
     1321 aggatgttcg tcatgtggtg cttgacggtc ttctcggaga tgaagagggc cgagccaatc
     1381 tccttgttgc tgtcaccagc ggcgacccgt tccagaatct ggcgctcacg ctcggtgagc
     1441 tcatcgagca gcttgcctcc ggcagacgag cgcgaacctg cgccggtcag ctcgaagagc
     1501 aacgttgccg ccagcgaagg cgtaacgtag acctcgccga cgtgaatcga gcgaacgatg
     1561 ttgaccagtt cacgcgccgc cacacccttg acgatgtaac cgcgcgctcc cgccttgagc
     1621 gcggcagtca cgtcctcttc atgctccgac gccgtcagca tgacgatcga tgtgaccggc
     1681 acactcgccg cgatcttgcc gacagcttgc aacccgccac ccggcatcga gatgtcgagc
     1741 aggatggtgt cgggcaggaa tgcctgcgcc aggcgaagcg catcatcagc gctcgcaccc
     1801 tcaccgacga cctcgatgtc cggctgggcg ctcagcgtat tgacgacacc gtcacggaac
     1861 aacgggtggt catcgacgat cacaacctgg atcagttcgt gcggttcacg catggaccaa
     1921 ctcctcggct cggtcctcgg caggtacagt ctccaccggc aggtgcgcga tgacggttgt
     1981 cccacgcccc ggtgcactct cgatcacaag cattccgcca agactttcga cccgctcgcg
     2041 cataccgacc agccccatgt gctcgtcaga cgccagcacg cgcgtcgcgt cgaagccgtg
     2101 gccgtggtcg atgacctgaa cagtgaacga agcatcgtca accccgaccc gtacctcctg
     2161 ctggatgccg cccgcgtgtc ggacgccgtt cgtcagcgat tcctgaatca cgcgatagat
     2221 cgcaatcttg gtcgcgacgg gagcatcgtc cggcaatggg ccgaggacga cgctcacctg
     2281 cgtgcccgat ctgcgttcgt gcgcccgaac cgcatgccgg acagtgtcgg cgagcggcag
     2341 cttgcttagc tggggcagca tcaggccggc cgaggtcgtc ctgatctcct gcagcgctcg
     2401 ccgaaccgat tgctgaatcg tatcgagctg ccgcgtgcac gatgcggcat ctggaagccc
     2461 accacaggag acgatgacgt tgtcgagttc gagcagagcc agactcagat cctgggccgg
     2521 gccatcgtgc aactccgaac tgaaccggcg caggaaccgt tcgttgaggg cggtcgttcg
     2581 cgctgcagcg cggcgaacgc gatcatggag ctgctcgttc tgctgcaaaa gatcggtgag
     2641 ccgatcgacc tgcgattcca attcacctcg ctggcgcacg atcgtattgc tggcgcgctg
     2701 gacgaagccc gccagcaaca ggtacatgac cagcgtcgca ccaccgacga tcagccagct
     2761 cgtgcgggtg gcagatgtga gttctcgatc gagcgcgtcc gtcacctgat agaactccgc
     2821 gactgcgatc acatcattcg tcccccgtaa ccgaaccgga gtgtagattt caagcagtcg
     2881 cgcctgactc tggcgctcca tctcgttttc ctcagcatcg agatcgctga tgcctgccgt
     2941 gaccgctccg tcccacgatt cccgaaggcg tgggtggacc gggtacacct tcccgatgac
     3001 ggacggttcc gtgctgtaga cgaccaggcc accgggtccc cacaccttga aggccgcgat
     3061 ctgctcccca agcggcgtat cggcaaacag gtggttgagc tcgttctgtt cagccgccga
     3121 gatggtcgag ccggtcgcca ttccctgcag gtgcggggcg acgacgctgt caacgtagag
     3181 cgcggtcgtc gccgaggttc gatggacgac gcccgactcg atccggttgg caacccaggt
     3241 tccaacgccg aacatccccg tgacgagaat gacgaggctg gccagcatga accgctgggc
     3301 gaagctgagg cgtcgaagtg gttggagcaa ggcgcggatg gctgaccgca tggcgatcct
     3361 cgttcaagtt ccagggcatg catcgcagtt acgtcgagta tgtcacagac cctggagcgg
     3421 acggtatcgg accaaggtcg cattggacat tcccgttcgg aagcgcgaag ttcggcgatc
     3481 tcgacatctg ccggctgggt cggatgggac cttagtccag cggccgatca gttcgagagt
     3541 cggatgtcgt cgctcgacca acgccggtta gcagaaccac gttcaccaca cagaatcagg
     3601 agcacaggat caacggcgga gaggttccgc aagatggaaa gggagtcttc gatgacacgc
     3661 acacagctgc ggtggatggt tccggttctc gcgctgatcg gcctgctggt gttctcagtc
     3721 tcggctgatg cgaagggggc tgaaacgatc gttcggctca aggggagttc gtcgtatcca
     3781 ggcgtctccg gcaaggcaaa gtacaaggtc gacggaactc agcgggagtt cgaagtcgag
     3841 atcgaggacg cgaaccggct catcggcaag acgctgacgg tcagcgtcaa cggcaagacg
     3901 gtcggcacga tgcgcgtcaa cagcttcggg agcgccgcgc tggagcgcaa cacgcgcctc
     3961 cgtcagaccg ttccaacaat cacgtccggc gcgaccgttc gaatcacgac atcgaccggc
     4021 gtcctggtgg catccggcag attctgatcc cactctcatt ctgatagccc gagacggcgc
     4081 ctcgcctggt tcgccagcgc gaggcgccat gctgtcacga ggatctccgg tccgggcatc
     4141 tgcgggtatt tcgaatgcgt tagccaggtg ctacgatgcc gcccatcgtc gaagtgtctg
     4201 gatgggaggc agcagggatg gcggagaagt caacggtcag tctgagcgtc gaacgcaacg
     4261 tggccgtgcc gatgcgcgat ggcacgatcc tctacgccga tgtctaccgt ccagccgggc
     4321 cggggccgtt tccagcgcta ctgactcgca gcccgtacga caaggccgtc accggcgccg
     4381 ccccgttcac cgtccgggcc gcgacggccg gctacgccgt cgtcattcag gacgtgcgcg
     4441 gtcggttcga gtcggaaggc gagttctacg cattcgtcaa cgagcggcag gacggctacg
     4501 acacgctcga atggctggcg agccagccgt ggtgcgacgg caagatcggg atgttcggcg
     4561 gctcgtacgt cgggttgaca cagtggcagg cggcgctctc cggccacccg gcgctgaagg
     4621 cgatcgtgcc gaacgtgaca gccgccaact accacaacgg ctggacctac cagggcggcg
     4681 cgttcgagtt gcagttcaac ctctcgtgga cgctctcggt cctcgcgatc aatacgtcgc
     4741 tgcgtgccaa caacggcgac atcaactcgc ccgcgttcca ggcgatcatg gacgcctccg
     4801 acaacatgac gcccgagttc gagaagctac cgctgaccgg caacgaagtc ttcggcgagt
     4861 tcgcccgcta ctacgacgac tgggtcaacc acccgaccta cgacgatttc tggtccgggc
     4921 tggatgtctc gcgcggctgg gaaagcatca ccgccgccag cttctcgatg ggcggctggc
     4981 acgacatctt cctgggcggg acgatcgaca acttcgccgg gatgacggcg gcttccggcg
     5041 atggccgcca ccggctgatg atcggcccgt ggaaccacgg cgggatgcgg acaggcaacc
     5101 cgatcggcga cgagaacttc ggcatgcgct cgaccggcgc cgtcatcgac gagggcggaa
     5161 tgcacctgcg ctggtacgac cgctggctgc gcgacatcga caacggcatc gagaacgatc
     5221 cgcccgtcaa gctgttcgtg atgggcagca atcgctggcg cgaggcccag acctggccgc
     5281 tggagaacac cgactggcag gagtggttcc tgcacagcgg cgggacggcc aatacgttga
     5341 acggggatgg cgccctttcg cgcgaggcgc ccggcggcga accggcagat tcctacgtct
     5401 acaacccgcg caacccggtt ccgacccgtg gcggcgggct ctgctgcaac aacgtcttca
     5461 gcctcggcgg cgcctgggat cagcgcgagg tcgagcagcg cgaggatgtc ctcgtctaca
     5521 ccagcgctcc gatggagaag gcccgcgagg tgaccggacc gatcaaggtc atcctctacg
     5581 ccagctcgtc ggcgacggat accgacttca ccgccaagct ggtcgatgtt gggccgtgcg
     5641 gctacgctcg caacctgaca gacggcatca tacgtgcccg ctacagggag tcgatggcgg
     5701 aggggacact gatcacacct ggcgaggtga ttgagtacga gatcgacctc tgggcgacca
     5761 gcaatctgtt caaggaagga caccggatcc gggtcgagat ctccagcagc aacttccccc
     5821 gcttcgaccg caacccgaac actggcgagc ttcccggccg ctcgactgag atggtcagcg
     5881 ccctccagac catccaccac tccaccgaat acccatcacg gatcgtcctg ccggtcatca
     5941 acgatccggc cggcccgtaa cactggaagg accgcgtcac tgtgaccctg acactgattc
     6001 gcgaccgcgc gccggatgtg attctgaccg acgcccggat cacgacgatg gacgctgatc
     6061 taccggaagc cgaagccgtt gcgatcaagg acggccggat ccttgctgtc gggccggccg
     6121 gagcgattga ggaactggcc ggaccggcga ccgtcgtacg ccggcttggc gggcggcggt
     6181 tgatccccag catcaacgac acccacaatc acctgatgtc gatggggaag gtgctcagcg
     6241 aggtccagct ctatgacgcg cgctcgatcg acgacatcaa ggagcgggtg gccgaacggg
     6301 tcgcgtcggc gccaccgggc gcgtggatca ccggccgggg ctgggacgag agcctgctgc
     6361 gcgatggccg cttcccgaac cgccacgacc tcgacgacgt cgcgccgaac aacccggtcg
     6421 tcctcgaccg cgtctggaac atgctggtgg ccaacagcgc tgcgctccac gccgccgatg
     6481 ttggccgcga gacacccgac ccgccggcag atcagctcta tgcgggccgg atcgtgcgcg
     6541 acgagcacgg cgaccccacc ggcgtcttcc gcgaccgcgc caagcggctg atccaggatg
     6601 tgatcccgct cccgacagcg gccgatcgcg agtcctacat ccgaaccgcc tgcgccgagt
     6661 acaaccggat ggggatcacg tcggcagccg aaccgggcct gtttccggag cagctacgcg
     6721 cctaccagaa cgtgcgccgg gccggagacc tctcgatccg cgtctcgctc tgcctggccg
     6781 gctgggggtt cggaacggcc agcgacgacg caacggtcga gcagcgcatc gacgacactg
     6841 gcgtctacac cggctacgga gatgactggc tacgattcga cactgtcaag ttcatgcccg
     6901 acgggggcgt cggcgaccgg acggcactga tgttcgagca ttacgagggc gagccggaca
     6961 acaccggcca attcgtcgtc tccgagcggg atctattccg caacgtcgcc tgggtccacg
     7021 aacgcggctg gtcgatcgac tgccacgcct gcggcgaccg gatgatcgag atggtcgccc
     7081 gcgcctatgc cgccgcccag agcgcccgac ccgacgcaac gatccgtcac cgaatccatc
     7141 atgcttacct gccgacgcca acggcgctcg acctgatgcg cgagtaccgc atcccggcgc
     7201 tggcgacgat cccgttcatc cacaacctcg gcgagagctt cgtcaccagc ctcggcgagc
     7261 aacgggccgc ccgcgtcatg ccgctacgga cctacctcga cgccggcgtg ccgctggcgc
     7321 tcggctccga cgcgccggtg acgacccaca acccgttcgt cggcatctac agcgccgtca
     7381 cccgcaagac cgtttacggc cgacagctcg gggccgagga gtgcatcagc cgcgaggagg
     7441 cgctgcgcgc ctacacgatc gatgccgcgt gggtgacgtt tgaggacgac agcaagggca
     7501 gcatcgcgcc cggcaagctg gcagatctgg ccgtcctcga ccgcgacatc ctcagcgtcc
     7561 cggaggacga gatcccgatg acgaccagcc tgctgacgct ggccggtggc cgggcaatcc
     7621 acgaccggct cgtatagcga gggagcattc cgatggcact cgcctacgtt cgcccgcgcg
     7681 agagcacctt cgacgccaaa gtcctagcct gggtcgacct cgcgtcacag cgcgtcttcg
     7741 ccctggacgg cacactggcc ggcttgctgc gcctcagccc cgacgagcgc tcacggcaga
     7801 tcaacgcgct gcgccagcag gccgacgcca atctggccct ggatgaggtc gcgtttcttg
     7861 cgccggtcga tgagcaggag gtctgggcag ccggcgtgac ctacgagcgc agccgcgacg
     7921 cccggatgga ggaatccagc gtcgatgacg tctacgcccg tgtctacgac gccgaacggc
     7981 cggagatctt cttcaaggcg ccggcctggc gctgcgtcgg cgatttccag ccggtcgcga
     8041 tccggcacga ctcaacctgg gacgtgcctg agccggagct ggcgctggtc atcgacagtg
     8101 aaggaacgat cgccggctac acggtcggca acgacgtctc gtcgcgttcg atcgaaggcg
     8161 agaacccgct ctacctgccg caggcgaaga tgttcagcgg ctcatgtgcg ctcggcccgt
     8221 ggatcgtcct cgccaacgaa ctgcatgacc cgttggcgct cgatatccgg ctacggatcg
     8281 agcgtgatgg ccagccccac tggagcggcc agacttcgac gcgcagcttc catcgctcgc
     8341 tcgaccagct ggtcgagtgc ctctactcgg cgctcgactt cccggccggg gcggtcctga
     8401 tgaccggaac cggcatcgtc ccgccgtccg agttcacgct cgaaagcggc gataccgtca
     8461 gcatcgagat cgacgggatc gggacgctga cgaaccacgt ctaccgactc ccggaacgga
     8521 gcgccaatgg ctgagcgtgc gcaggtcatc gactggacgg gcatcgaagc cagcgaaccc
     8581 ttccccggca tcgtccgcca ggcgcttgtg acggcgacga gcacgatcgt ccgctacacc
     8641 tatcaccccg gttgcgtctt cccggtccat caacatcccg aggagcaact cacgctcgtt
     8701 cacagcggcg agatcgagtt tgaggtcgcc gatgagccgg tcatcctccg cgccggccag
     8761 atcgcggtca ttcccggcgg tgttccgcac ggcgcgcgtg tcacggcaag tgaggtcgtc
     8821 atcaccgaca actacatcgc aagtgcgaat cggacgccgt tgcagtttga agggggccag
     8881 tcatgagccg cttcgtcacc tccgaatggc tgatggacaa gaccggcgcg agcagcgacg
     8941 gcgggatggt tgtcgccaag caggagatcg cggcgatggc cggtgcgcag gtgctggcag
     9001 agggcggcaa cgccgtcgac gccgccgtca cgaccgcctg ggtgatgaac gtgatggaac
     9061 cttacaactg cacgatcgcc ggggccggct acctcgtata ccgggccgca tccggagaat
     9121 cctgggtcgt cgatttcagc aaccgggcgc cctcccgcgc cacgcctgag gcgctggcgt
     9181 cccggacgac cgtcgccttc gccgggccgc tggccgccgc tgtcccggcg accgttgccg
     9241 ggctggcaac cgcgctggag cgcttcggca cgatctcgct ggcccgctca ctggcgccgg
     9301 ccatcgaagt cgccgccgag ggcatgccgc tcaactggct gctgacgttg cgactggttc
     9361 aggaactacc cggcatccgc gccaacccga agacggccga gatcttcctc accaacggcg
     9421 accccggcat ggcccacggc gagacggtcc tgcgtcaaac cgatctggcc cggacgctgc
     9481 gggccatcgc cgacgacggc atcggcgcgt tctacgaggg cgagatcgcc agcaagatcg
     9541 tcgcgttcgt caatgaccag ggcggcatcc tcgacgcagc cgatttcgtc cgattccagc
     9601 cgaccgtcgt cgagccgctc cgcgcgtcgt accgcgacta tacgctgctc ggcgtcccgc
     9661 tcccgtcgcc ggggctgacg acgatccaat gcctgcggat gctggcgggc gacgatctgg
     9721 acgcgatggg ccacaacagc gccgacgcgc tgcatctgtt ggccgaggca ttccggctcg
     9781 ccttcgccga ccgcgacgcc tacctcggag acccggagtt ctccgacccg ccggtcgaag
     9841 cgctgctgtc cgacgactac atcagccgcc gccgtgccga gatcgactcg cgccgcgcga
     9901 tggaggtggt tcgccccggc gacgttggcc gggtaccgct cggcgccgag ggcggcggtg
     9961 gcgggacgac ccagttctgc gcggtcgatg cggccggcaa catggtgtca ctcactcaga
    10021 cgctgatcgg cggcctaacc ggcctcggcg tcgccggtga cacgggcgtc gtcatgaact
    10081 gcgccctgca gtggttcgac ccgcgtcccg gcgcaccgaa cagtgtcgca cccaacaagc
    10141 gcccgctctc gaacatgaca ccgatgatcg tcgaacgcga cgggaagcct gtcctggccg
    10201 tcggcgcacc gggttcacgc cggatcacca acgccgtcac gcaggtgacg ctcaacgtcc
    10261 tcgagcacgg cttatcggcc caggcagcca tctccgcacc ccggatcgat tgctcgctgg
    10321 ggcacattct ggctgacgac cggatcgacg cggcgacgat cgccgacctg cgcgaacggg
    10381 gccatcgcgt cgatatcgtc aatgagttca tcaactccgg cggcccctcc actggctacc
    10441 gtggcaactt ctcccgcccg gccgcgatcc acatcgacgc cgagggcatc cgccacggcg
    10501 gtgactaccc cttcgtcgaa gggatggccg tcggcgtgcc gcgtgatgca tgacgcgcca
    10561 tcaatcggcc aatcaatgcg taggggcgat gtcggtgact cgccctcaat atcaatcgtc
    10621 caatcc
//
LOCUS       DASUGJ010000531         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1334758, whole genome shotgun sequence.
ACCESSION   DASUGJ010000531 DASUGJ010000000
VERSION     DASUGJ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334758"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFV93_12025"
     CDS             <1..608
                     /locus_tag="VFV93_12025"
                     /inference="COORDINATES: protein motif:HMM:NF037737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166117.1"
                     /translation="VATYAKDDGVHLRITAISADADSATRAVDQAEAEIRAIVGNHVY
                     GYLDDPISTVILRLVAAQGQRIAVWEAGNAGHLGVLLEESDLGDRVVADARTTSFEAA
                     VAEIGSGDDPEQVAIACATLAANRAGTIHGAAIAIRLSPGENPDRASGEIAIALTING
                     QARVRRNTVSAVTPEIRRRATLQAVDFLWSTLRDETARQPG"
     gene            complement(517..1602)
                     /locus_tag="VFV93_12030"
     CDS             complement(517..1602)
                     /locus_tag="VFV93_12030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5166118.1"
                     /translation="MNERPAVSLVATVYNEAKTIDEWLAALLAQTRQPDEIVIVDGGS
                     DDATVANIEALQRASSVPIHLSVAPGANISEGRNLAIARATGRLIAVTDAGTLADPRW
                     LERLIAPLAAGEADVASGFFVPRLTTGWERALAAATLPDLGDIDPDRFLPSSRSVAFR
                     REWFDAGLRYPEWLDYCEDLVWDLAMQRAGARFRFAPDAIVTFVVRRSPAAFALQYAR
                     YARGDGKAGLFARRHALRYVTYAGLALVLRRRSVPLVSLACLLGGAYVSRPVKRLRER
                     DRQSAVEARETLKALPLVVASRGLGDVAKMVGYPAGLFWRWRRFGGIGPQTAWRRVSP
                     AGELFRPAALTRESQPPAVSPAAESQA"
     gene            complement(1599..2060)
                     /gene="ybeY"
                     /locus_tag="VFV93_12035"
     CDS             complement(1599..2060)
                     /gene="ybeY"
                     /locus_tag="VFV93_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX5166119.1"
                     /translation="MSLRCDVLVDPGVTFKHGAALIDLIQFAAEREPSLPADEWHMTL
                     RLSDDATIAALHEQFFNDPTATDVITFPAGDGDDGGEAYLGDVVVSVETAARQAGEGD
                     HSVAREIAFLGLHGLLHLTGYNDPDDEQRVAMHRRQLQHLEAWEASRGRPW"
     gene            complement(2064..>2479)
                     /locus_tag="VFV93_12040"
     CDS             complement(2064..>2479)
                     /locus_tag="VFV93_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166120.1"
                     /translation="IRYFYVKSVEAGEDVTEEEFRYPGPKPQTKEAGVVMLADSIEAA
                     VRAAAGGGRLASAKNAGREGTDTLTGIVDKIIAERLDDGQLNECDLTLREIEMIRRTF
                     VQILEGIYHPRVEYPSMPVKEAPASPVTPAATAAN"
BASE COUNT          437 a          875 c          777 g          390 t
ORIGIN      
        1 ccgtcgccac ctacgccaag gacgacggcg tccacctgcg catcacggcg atcagcgcgg
       61 acgccgactc ggccacgaga gccgtcgacc aggccgaggc cgaaatccgc gccatcgtcg
      121 gcaaccacgt ctacggctac ctcgatgacc cgatctcaac cgtcatcctg cgcctcgtcg
      181 cggcccaggg acagcggatc gccgtctggg aggccggcaa cgccggacac ctgggcgtcc
      241 tgctcgaaga gagcgacctg ggggatcggg ttgtcgcgga cgcgcggacg acgtcgtttg
      301 aagcggccgt cgccgagatc gggtccggcg acgacccgga gcaggtcgcg atcgcctgcg
      361 cgacactcgc cgccaaccgc gcgggaacga tccacggtgc cgccatcgcc atccggctgt
      421 ctcctggcga gaatcctgac cgagccagcg gcgagatcgc gatcgcgttg acgatcaacg
      481 ggcaggcgcg tgttcggcgc aacactgtca gcgccgtcac gcctgagatt cggcggcggg
      541 cgacactgca ggcggttgac tttctctggt caacgctgcg ggacgaaaca gctcgccagc
      601 cgggctgact cgccgccagg ccgtttgcgg cccaatcccg ccgaatcgcc gccagcgcca
      661 gaacagccca gccggatatc cgaccatctt ggcgacatct cccagcccac gcgatgcgac
      721 gaccagcggc agcgccttca acgtttcgcg ggcttcaact gccgattggc gatcgcgctc
      781 ccgaagtcgt ttgaccggtc gcgagacgta tgcgccgccc agcagacagg cgagcgacac
      841 cagcggaacg ctccggcgac gcaggacgag cgcgaggcca gcgtatgtca cgtatcgcag
      901 tgcgtggcgg cgggcgaaca accccgcctt gccatcgccg cgcgcgtagc gtgcgtactg
      961 tagcgcgaac gcagccggcg aacggcgcac cacaaacgtc acgatcgcat ctggagcgaa
     1021 cctgaagcgt gcccctgcgc gctgcatggc gaggtcccag acgagatcct cacagtagtc
     1081 gagccactcg ggatagcgca gcccggcatc gaaccactca cgccgaaatg cgaccgatct
     1141 gctggacggc aggaaccggt ccgggtcaat atcgcccaga tccggcagcg tcgcggccgc
     1201 cagcgcccgt tcccagccgg tcgtcagacg cgggacgaag aatccggacg cgacatcggc
     1261 ctcgcccgcc gccagcggcg cgatcagtcg ctcaagccag cgcggatcgg ccagcgtccc
     1321 ggcgtccgtg acggcgatca accggccagt cgctcgcgca atggccagat tccgcccctc
     1381 ggagatattc gccccgggcg ccacactgag gtgaatcggt accgacgacg cgcgctgaag
     1441 tgcttcgatg tttgcgactg tcgcatcgtc cgacccacca tcgacgatga cgatctcgtc
     1501 cggttgccgg gtctgcgcca gcagcgcagc cagccactcg tcaatggtct tcgcttcgtt
     1561 gtagacggtg gcgacaagcg aaaccgccgg gcgctcgttc accacggcct gcctcgcgac
     1621 gcttcccacg cttccaggtg ctgtagctgc ctccggtgca tggcgacgcg ttgctcgtcg
     1681 tccggatcgt tgtatccagt cagatgcaac agcccgtgaa gtccaaggaa cgcgatttct
     1741 cgagcgaccg agtgatcgcc ctcacccgcc tgccgggccg ccgtttcgac ggagacgacg
     1801 acatcgccga ggtacgcctc accaccgtcg tcgccatcac cggccggaaa cgtaatcaca
     1861 tcggttgcag ttggatcgtt gaagaactgt tcgtgcagcg cggcgatcgt cgcatcgtcg
     1921 ctgagccgca gcgtcatgtg ccactcatca gccggcagag acggttcccg ctcggccgcg
     1981 aactggatca ggtcgatcag cgccgctcca tgcttgaacg tgacaccggg atcgaccagt
     2041 acatcacaac ggagcgacat cgatcagttc gcggccgtcg cggccggtgt caccggcgag
     2101 gcgggcgcct ctttcaccgg catcgaaggg tactcaacgc gggggtggta aatgccttcg
     2161 aggatctgga cgaacgtgcg tcggatcatc tcgatctccc gcagggtgag gtcgcattca
     2221 ttcagctgac cgtcgtccag ccgctcggcg atgatcttgt cgacgatccc ggtcagcgtg
     2281 tcagtccctt cgcggccggc gttcttggca ctcgccagcc gtcctccgcc ggccgctgcc
     2341 cgaacggcag cctcaatcga gtcggccagc atgacgaccc cggcctcctt cgtctgcggc
     2401 ttcggaccgg gatagcggaa ctcttcctcg gttacgtcct cgcccgcctc gaccgacttg
     2461 acgtagaagt agcggatca
//
LOCUS       DASUGJ010000532         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1890344, whole genome shotgun sequence.
ACCESSION   DASUGJ010000532 DASUGJ010000000
VERSION     DASUGJ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890344"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2053
                     /locus_tag="VFV93_12045"
     CDS             <1..2053
                     /locus_tag="VFV93_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5166121.1"
                     /translation="SASRRALLDLSQALADETDAEAVANRLATDLDTILPSASSGVLL
                     PLPSGELEIVATCGADAEARRGKRIPAGAGVTGHTYREGVPTIVDDVDADPRHISLRK
                     EVGSELAVPLRYRDRTIGVLNFERSQRHAFTPSDVALAQIVAAHAAQAIARANLITRL
                     RRQNVELEIANRHKGEFLANMSHELRTPLNAIIGFSELLLDDPDGIYDLETTSRFLST
                     IHSSGEHLLSLINDILDLSKVEAGQMSFNPEPASLPHLIDQVMATMRPLADRKSIAMT
                     ADARAGAQIVADTGKVKQILYNLLSNAIKFTPDEGRVSVTTRQHDDGILLTVADTGIG
                     IAADDLERVFDEFQQIEGGPGRHYEGTGLGLALTRRFVTMHGGRVWVESVPGEGSQFH
                     ALFPETSVETIQPEPAEPDATDSQPAPSRLVADGEPLILVVEDDERAASLLSHYLGRG
                     GYRTTIAADGSVALQWARDLRPAAITLDVMLPSLDGWEILRSLKLDPLTRDIPVVIAS
                     IIDDSDLGFALGATDYFVKPVDRQALLARLDRYVRPGPAHDRQLTILITDDDPVAIDL
                     LENMLAPAGYATLAANGGEAAIAAAAEHSPDIVLLDLMMPGVDGFAVVDALRANPTTR
                     DTPILIITAKELTDADKQRLNGRVTTILRKGTFGAIELIAWLDTTLGQPAHHKGDADG
                     S"
     gene            2043..2453
                     /locus_tag="VFV93_12050"
     CDS             2043..2453
                     /locus_tag="VFV93_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5166122.1"
                     /translation="MDRDQTAGLVLIVEDNPANLLLARVMLERSGWQISEARSADEAR
                     QTLQTTTPDVILMDVGLPGQDGLSLTSELKSASDTSAIPIVALTAHAMQSDREKAFAA
                     GCDGYLSKPFKAQQLRDEISRVLQTSSRADRQAD"
     gene            complement(2465..>3017)
                     /locus_tag="VFV93_12055"
     CDS             complement(2465..>3017)
                     /locus_tag="VFV93_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5166123.1"
                     /translation="WNGERVSDPAAVLVPPRAAYTAQAPRLFSDTLRQNILLGQPDDP
                     QALEAAIHGAVMEDDVRGLEAGLETLVGSGGVKLSGGQLQRAATARMLVQNAELLVID
                     DVSSALDVNTERQLWERLFAGRDVTCLAVSHRRAALRRADHVIVLQDGSVTAYGTLDE
                     LLATSAEMRALWHEADEPDASAS"
BASE COUNT          527 a         1071 c          941 g          478 t
ORIGIN      
        1 gtcggccagc cgccgcgcgt tgctcgatct gagtcaggcg ctggccgatg agaccgacgc
       61 ggaggccgtc gccaaccgcc tcgcgaccga cctcgacacg atccttccgt ccgcatcatc
      121 cggcgttctg ctgccactcc ccagcggtga gctggagatt gtcgcgacct gcggggctga
      181 cgcggaagcc cggcgcggca agcggattcc ggccggagca ggcgtgaccg gtcacaccta
      241 ccgcgagggc gttccaacca ttgtggatga tgtcgacgcc gatccgcgcc acatcagcct
      301 gcggaaggag gtcgggtccg agctggcggt cccgttgcgc taccgcgaca ggacgatcgg
      361 cgtcctcaac ttcgagcgca gccagcgcca cgcgtttacg ccgtccgatg tggcgctggc
      421 gcagatcgtc gccgcccacg ccgcccaggc aatcgctcgt gccaacctta tcacccggct
      481 ccggcggcag aacgtcgaac tggagattgc caatcgccac aagggcgagt tcctcgccaa
      541 catgagccac gaactgcgca ccccgctcaa cgccatcatc ggcttctccg agctgttgct
      601 ggacgatccg gatggtatct acgacctgga gacgaccagc cggttcctca gcacgatcca
      661 ttcgagcggc gagcacctgc tctcgctgat caacgatatc ctcgacctct ccaaggtcga
      721 ggctggccag atgagcttca atccggagcc ggcctcgctg ccccacctga tcgaccaggt
      781 gatggcgacg atgcgtccgc tggccgatcg caagtcgatc gcgatgacgg ccgatgcccg
      841 agccggcgcg cagatcgtcg ccgataccgg caaggtcaag cagatcctct acaacctgct
      901 ctccaacgcc atcaagttca cgccagatga gggcagggtg agcgtcacga cccgccagca
      961 cgacgacggc atcctgctga ccgtcgccga caccgggatc ggcatcgccg ccgatgacct
     1021 ggaacgggtc tttgacgagt ttcagcagat cgaaggtggt ccgggccggc actacgaggg
     1081 caccggcctc ggcctggccc tgacgcgccg tttcgtgacg atgcacggcg ggcgtgtctg
     1141 ggtcgaaagc gtccccggcg agggcagcca gttccacgcc ctctttcctg agacgtcggt
     1201 cgagacgatc cagcccgaac cagccgagcc cgatgctacc gacagccagc cggcgccgtc
     1261 ccggctggta gcggacggag agccgctgat tctggttgtc gaggacgacg agcgggccgc
     1321 cagcctgttg tcgcactacc tcgggcgcgg cggctatcgg acgacgatcg ctgccgacgg
     1381 tagcgtcgcc ctccagtggg cacgcgatct gcgcccggcc gccatcacgc tggatgtcat
     1441 gctgccgtcg ctggatggct gggagatcct gcgctcgctg aagctcgatc cgctgacgcg
     1501 tgacatccca gtcgtgatcg cctcgatcat cgacgacagc gatctcggct tcgccctcgg
     1561 cgcgaccgac tacttcgtca agcccgtcga tcgccaggcg ttgctggccc gcctcgatcg
     1621 ctacgttcga ccggggccgg cccacgatcg ccaactgacg atcctgatta ctgacgacga
     1681 tccagtcgcg atcgacctgc tggagaacat gctcgcgccg gccggctacg ccacgctggc
     1741 ggcgaatggc ggcgaggcag ccatcgccgc tgctgccgaa cacagtcccg atatcgtcct
     1801 gctcgacctg atgatgccgg gggtcgatgg gttcgccgtc gtcgacgcgc tgcgcgcaaa
     1861 tcccaccact cgcgacacgc cgatcctcat catcactgcc aaggaattga ccgacgctga
     1921 caagcagcgc ctgaacggcc gcgtcacgac gatcctgcgc aagggtacgt tcggcgcgat
     1981 cgagctcatc gcctggctgg acacgacgct gggccagcct gcacatcaca agggagatgc
     2041 cgatggatcg tgatcagaca gccggactcg tcctgatcgt tgaggataac ccggccaacc
     2101 tgctgctggc gcgggtgatg ctcgaacgct ccggctggca gatcagcgag gcccgctcgg
     2161 ccgacgaggc ccgccaaacc ctccagacga cgaccccgga tgtcatcctg atggacgtcg
     2221 gcctgcccgg tcaggacggt ctcagcctga ccagcgagct gaagtccgcg tcggacacga
     2281 gtgcgatccc gatcgtcgcc ctgacagccc acgcgatgca gagcgaccgc gagaaagcgt
     2341 tcgccgccgg ctgcgacggc tacctctcca agcctttcaa ggcccagcaa ctgcgcgacg
     2401 agatttcccg cgtcctgcaa acctcgtcgc gtgccgaccg acaggccgac taaccggcat
     2461 tcgcctacga cgcagaggca tccggctcgt cggcctcgtg ccagagcgcg cgcatctcgg
     2521 cgctcgtcgc cagcagctcg tcgagcgtgc catacgcagt cacgctcccg tcctgaagga
     2581 cgatgacgtg atccgcacga cgcagggcgg cgcgacggtg cgagacagcc agacaggtga
     2641 cgtctcggcc ggcaaacaac cgttcccaga gttgccgctc cgtgttgaca tccagagcgc
     2701 tggacacgtc gtcgatcaca agcagctcgg cgttctgcac cagcatccga gccgtcgccg
     2761 cgcgctgaag ttgcccgccg gagagcttga cgccgcctga gccgaccagt gtctccagcc
     2821 ctgcctcaag cccccgcacg tcgtcttcca taaccgcgcc gtggatggca gcttccagtg
     2881 cctgcggatc atcgggctga ccaagcagga tgttctgccg gagcgtgtcg ctgaacagcc
     2941 tcggcgcctg cgccgtatac gccgcgcggg gcggcacgag gacagcggct ggatcgctta
     3001 cgcgctcgcc gttccag
//
LOCUS       DASUGJ010000533         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_84079, whole genome shotgun sequence.
ACCESSION   DASUGJ010000533 DASUGJ010000000
VERSION     DASUGJ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84079"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VFV93_12060"
     CDS             <1..1042
                     /locus_tag="VFV93_12060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166124.1"
                     /translation="PSGCGKTTTLRMIGGFEEATSGTIYLGEADVTGLPPYKRDVNTV
                     FQNYALFPHLTVFDNVAFGLRRKKVDDGEVKRRVGEMLDLVEMPGYERRKPGQMSGGQ
                     QQRVALARALINHPRVLLLDEPLGALDLKLRKQMQLELKRIQTEVGITFIYVTHDQEE
                     AMTMSDRIAVMRAGRIEQLGTPEELYERPETAFVAGFLGVSNLLEGEVTGRDGALVSV
                     RLSDGTVLHAPANGTSAGGRVRLGVRPEKLRVDAGEHAGATNGLNALSGTVLDASYIG
                     VSTQYIIQTAEGHRLSVYAQNLDTAGAAELLADGQRVVLTWKPQHTFVIGSQAEPVNS
                     DPHTSEEGEIDE"
     gene            1035..2309
                     /locus_tag="VFV93_12065"
     CDS             1035..2309
                     /locus_tag="VFV93_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5166125.1"
                     /translation="MSDPERSQIELAIERLLQGDPISRRAFLRRTGRGGVALGAAFSI
                     PALLAACASPGASGSGGGQATLEWANWPLYIDIDEEAAPDPTPYPSLNAFIRETGINV
                     HYVEAINDNEEFYGTIQPDLAAGNPTGWDIVSPTSWMVKRMADAGYLEELDHSKLPNW
                     TANCADYAKGLWFDPDNKYSVWWQGGITGIGYDPNLTGREITKFDDLLDPAFAGRVGG
                     FSDMRDMMTMCLASLGIDPTTATVEQAQQAKDKLLAAAQAGQFRGFYGNEYYDALAAG
                     DLVASVAWSGDITQMQLNDNDQVQFVVPSDGAPRWNDNLVIPKNAALKEQAHTLINYW
                     YDPAAATTLSEWIGYFTPVVGVSDLIQADADAYREDGDTENADYYDAVAPSVTPTPDQ
                     LAGTYDYPQFENPEDEQAWNDMFLEVTTAAGS"
BASE COUNT          431 a          828 c          751 g          332 t
ORIGIN      
        1 cccttcgggc tgcggcaaga cgaccaccct gcgcatgatc ggtggcttcg aagaggccac
       61 cagcgggacg atctaccttg gcgaggccga tgtcaccggg ctgccgccgt acaagcgtga
      121 cgtgaacacg gtcttccaga actatgccct cttcccgcac ctcaccgtct tcgacaacgt
      181 cgccttcggg ctgcgccgca agaaggtcga cgatggcgag gtcaagcgcc gcgtcggcga
      241 gatgctcgac cttgtcgaga tgccgggcta cgagcgccgc aagccgggcc agatgtccgg
      301 cgggcagcag cagcgcgtcg ccctggcgcg cgccctgatc aaccatccgc gcgtcctgct
      361 gcttgacgag cctctcggcg ccctcgacct caagctgcgc aagcagatgc agctggagct
      421 gaagcgcatc cagaccgagg tcggcatcac cttcatctac gtcacccacg accaggaaga
      481 ggcgatgacg atgtccgacc ggatcgccgt catgcgcgcc ggccggatcg agcagctggg
      541 cacgccggag gagctgtacg agcggccgga gacggcattc gtggcgggct tcctgggcgt
      601 ctccaacctg ctggagggcg aggtcacggg ccgcgacggt gccctggtct ccgtccgcct
      661 gtccgatggc accgtgcttc acgcccctgc caacggcacc tccgcgggag ggcgggtgcg
      721 cctcggcgtc cgaccggaaa agctccgcgt cgatgcgggg gagcacgccg gcgccacgaa
      781 cggcctcaac gccctgagcg gcaccgtcct ggacgcgagc tacatcggcg tcagcaccca
      841 gtacatcatc cagaccgccg aagggcatcg gctatcggtc tatgcccaga acctcgacac
      901 cgccggggca gcggagctgc tcgccgacgg ccagcgcgtc gtcctcacct ggaagccaca
      961 gcacaccttc gtcatcggca gccaagccga gccagtcaac agcgacccgc acacgtccga
     1021 agaaggagag atcgatgagt gacccggagc ggagccagat cgagcttgcc attgagcggc
     1081 tcctccaggg ggatccgatt tcacggcgtg cctttctgcg ccgcaccggg cgcggcggtg
     1141 ttgcgcttgg cgctgcgttc tcgatcccgg ctctgctggc ggcctgtgca tcgccgggag
     1201 cttccggctc cggcggcgga caggcgaccc tggaatgggc gaactggccg ctctacatcg
     1261 acatcgacga ggaggccgcg cccgacccga cgccctaccc gtccctcaat gcgttcatcc
     1321 gcgagaccgg catcaacgtc cactacgtcg aggccatcaa cgacaacgag gagttctacg
     1381 gcaccatcca gccggatctg gcggccggca acccgaccgg ctgggacatc gtcagcccga
     1441 ccagctggat ggtgaagcgc atggccgacg ctggctacct cgaggagctg gaccacagca
     1501 agctgccgaa ctggacggcg aactgtgccg actacgcgaa gggcctctgg ttcgatcccg
     1561 acaacaagta cagcgtctgg tggcagggcg ggatcaccgg catcggctac gacccgaacc
     1621 tcaccggccg cgagatcacg aagttcgacg acctcctcga cccggcgttt gcgggtcgcg
     1681 tcggcggctt cagcgacatg cgggacatga tgaccatgtg cctggcgtcg ctcggcatcg
     1741 acccaaccac cgccacggtt gagcaggcgc agcaggcgaa ggacaagctc ctggccgccg
     1801 cgcaggccgg ccaattccgc ggcttctacg gcaacgagta ctacgacgcg ctggccgccg
     1861 gggacctggt cgcctccgtg gcctggtccg gggacatcac ccagatgcag ctcaatgaca
     1921 acgaccaggt ccagttcgtc gttccctcgg acggcgcgcc tcgctggaac gacaacctgg
     1981 tgatcccgaa gaacgcagcc ctcaaggagc aggcgcacac cctgatcaac tactggtacg
     2041 accccgccgc ggccacgacc ctgtcggagt ggatcggcta cttcacgccg gtggtcggcg
     2101 tcagcgatct gatccaggcc gatgcggacg cctatcgcga ggacggcgac acggagaacg
     2161 ccgactacta cgacgcggtg gctccctcgg tgacccccac ccccgaccag ctggccggca
     2221 cgtacgacta cccgcagttc gagaaccccg aagacgagca ggcctggaac gacatgttcc
     2281 tggaggtcac gaccgccgcc ggcagctgac gaacgcgcct gaacccgaga tcggacgcga
     2341 ga
//
LOCUS       DASUGJ010000534         5003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1752252, whole genome shotgun sequence.
ACCESSION   DASUGJ010000534 DASUGJ010000000
VERSION     DASUGJ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752252"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFV93_12070"
     CDS             <1..462
                     /locus_tag="VFV93_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5166126.1"
                     /translation="DAATCLSATEEEFVELLNAYRADLGLQPVVASAKLTLAAYRHSL
                     DMGTRNYFAHDTKEPIPAGQSGPSYQDRIRDAGYTGWTIAMESIAGGNQVYADAEALF
                     DVLLGIPEDYQHMMDPNITQIGIGQAQVFGGSYRYLWTVEYANGNDGPPGC"
     gene            complement(716..1261)
                     /locus_tag="VFV93_12075"
     CDS             complement(716..1261)
                     /locus_tag="VFV93_12075"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX5166127.1"
                     /translation="MTTTRPSRLQGYRQRRDEFMRTNEHSPLTDRQRNEFVPLDYFEE
                     NPDLVFVLDVDRDVPTDPVELPTTSGETKPYQPYGRVTFEVDGQPATLMVYREPERGR
                     LILPFRDGTCGQEVYGAGRTVDPQERPDGKLSIDFNYAYGPYCQYNDNWVCTIPPMEN
                     WLRVPIRAGEKRYPGKESSQH"
     gene            complement(1369..2190)
                     /locus_tag="VFV93_12080"
     CDS             complement(1369..2190)
                     /locus_tag="VFV93_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166128.1"
                     /translation="MNVRELVQRAIANHEGLSPAPFSDDMPDTTAYLATPDRLAYALG
                     WIVASAIVSARYASSGIDALPVFTPDHGWDRFLLTRRTTSDEFRRESANRYGMIMLSG
                     EDAPILTKPSGETKIALGTLLREDPERAIQEALALVPARALPIADLGKRWKERKVQYP
                     LLYETVAGIIADYPGTIAAREVFVDDQPVDGKYHPLYLHGVALQPALTYDWFLIQHGE
                     RAAFFRIHGGQSVYETDRGGWSTIKRQLAAEDEAGKRARMLSWLRLEGQPDKSVD"
     gene            2338..3645
                     /locus_tag="VFV93_12085"
     CDS             2338..3645
                     /locus_tag="VFV93_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166129.1"
                     /translation="MTEGDRSQRVLGIYQGWVVVIAVMLTLAIASGGRFLFGVVLIPV
                     TEEFGWSRAELTLAVTISVIALSILQPIIGLLVDRFGSRRILIAGVVTTAIAMIPITI
                     ATSLWQIYVFYGILAAFGFAATSPVNTTALVNGWFEKKRGLALSMATAGSAYGQLLII
                     PIATAIMVQWGWRASYYAITAALVLLVLPLALFFIRDSPSRTASRHSRQTGVFDGPRV
                     SLGQAVRTAAYWQISFGMFVCGATMGFTSVHMIPYLTDMPEHSTHTMQTTASLAMSVV
                     GGASIVGSLVLGYLSDRKGHRQILALTYFLRGLSFLVLLAVGSFIPGIFIAAIILGVS
                     WTSTTPLGSAIAADIFGRASMGTIFGFIFTSMNIGSGLGAFIAGLDQDVTGNYHAVLV
                     ANVALGFLAAIATWSIKVQPYEVPQTSVLRDRAEPALTRSIGD"
     gene            complement(3660..4658)
                     /locus_tag="VFV93_12090"
     CDS             complement(3660..4658)
                     /locus_tag="VFV93_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5166130.1"
                     /translation="MGTIQRDDHRIEGRDGMTLAVRRVWALGATSAATPLILVHGARA
                     GGVASFDVPVEGGSLAADLAGAGYDVFVMDARGYGGSSRPPEMEQPATASAPLVRSVD
                     VVRDLDAVVDWVRAVTGEERVSLLGWATGGHWAGFYASLHPDQVKQLIIFNTLYGSTF
                     DHPSLGRGSGMEDAANPGRFDRAGTGGWRSSAAASLLRTWDNSIPEGELDRWRDPAVA
                     AAYARAAIEAATGDPDATAFPHPTGALEDSFYLATGRQLWDASFVRAHTLVIRSERDF
                     WSRPEDVERLAEQLIDAASVRTVTLLNATHFAHLDRPEYGRRQFLDEVLSFLAETA"
     gene            complement(4704..>5003)
                     /locus_tag="VFV93_12095"
     CDS             complement(4704..>5003)
                     /locus_tag="VFV93_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5166131.1"
                     /translation="AIWFIPLLFLVIRPVSVLFSLVGSGVSQPERRLLGWFGMRGVGS
                     IYYLAYAVEHGLSVPDAEQLASMTLSVVAVSIVVHGISMTPLMRRHERRQERLVG"
BASE COUNT          892 a         1664 c         1551 g          896 t
ORIGIN      
        1 gacgccgcga cctgcctgag cgcgaccgaa gaggagttcg ttgaactcct gaatgcctac
       61 cgggctgatc tgggactaca accggtcgtc gcttcagcga aactgaccct cgcggcctac
      121 cgccacagcc tcgacatggg cacgcggaac tacttcgcgc acgacacgaa ggaaccgatc
      181 cccgccggcc agtccgggcc gagctaccag gaccgaattc gcgacgccgg ctataccggc
      241 tggacgatcg cgatggaaag cattgctgga ggcaaccagg tctacgccga cgccgaggcg
      301 ctctttgatg tcctgctcgg catccccgag gactaccagc acatgatgga cccgaacatc
      361 acccagatcg gcatcggcca ggcgcaggta ttcggcggca gctaccgcta cctatggacg
      421 gtcgagtacg ccaacggcaa cgacggcccg ccgggctgtt gatcgaacgt tccccctgtc
      481 gtcctgagtg gtccaccacg ctggatctgc cgtccccgta gggacaggtc tcagacctgt
      541 ccgcgccgtg aggcgcctgc ttggcaccgg tctggtcaat ccacgggcgc aggcgttggg
      601 gatccccgtg acacacgcat ggatcacgcg ggcgcctcac ggcgcggaca ggtctgagac
      661 ctgtccctac agagacggca gatccagcgt ggtggaccac tcaggacgac aggtactagt
      721 gctgcgatga ttccttgccg ggataccgct tctcgccggc ccggatcggg acgcgcagcc
      781 agttttccat gggtgggatc gtgcagaccc agttgtcgtt gtactggcag tacggcccat
      841 aggcgtagtt gaagtcgata ctgagcttcc cgtccggtcg ctcctgcggg tcgaccgtgc
      901 ggccggcgcc gtagacctcc tgcccgcagg ttccatcacg gaatggcagg atcaggcgac
      961 ctcgctccgg ctcgcggtag accatcagcg ttgccggctg tccgtcgacc tcgaaggtga
     1021 cgcgtccata gggctggtac ggcttggttt cgccagatgt cgtcggcagt tcaaccggat
     1081 cggtcgggac gtccctgtcg acgtccagca cgaagacgag atcgggattc tcttcgaagt
     1141 aatcaagcgg gacgaactcg ttccgctgcc gatcggtcag cggcgaatgc tcgttggtgc
     1201 gcatgaactc atcacggcgc tggcgatagc cctgcaggcg cgatggtctg gttgtcgtca
     1261 ttccccatgg ctcctttcgg ggcatccccg catacgggac ccaatacccc ctcggggtat
     1321 tacaacgctc ggacggtcca ggatattcct gcccggacgg ctgcggcgct aatcgacgga
     1381 cttgtccggc tggccctcaa ggcgcagcca cgacagcatc ctcgcacgct tgccggcctc
     1441 gtcctcggcc gcgagttgcc gcttgatggt ggaccagcca ccccggtccg tctcgtagac
     1501 cgactggcca ccgtggatgc ggaagaatgc agcccgctcg ccgtgctgga tcaggaacca
     1561 gtcgtaggtc agcgccggct gcagcgcaac gccgtgcagg tacagcgggt gatacttccc
     1621 gtcaaccggc tggtcatcga caaacacttc ccgggcagcg atcgtgccgg gatagtcggc
     1681 aatgatgccg gcgaccgtct cgtagagcag cgggtactgc accttgcgct ctttccagcg
     1741 cttgccgaga tcggcgatcg gcaacgcgcg cgccggaacg agcgcgaggg cctcctgaat
     1801 cgcccgctcc gggtcttctc gcaggagtgt gccgagcgcg atcttcgtct cgccggatgg
     1861 cttggtgagg atcggtgcgt cctcgccgct cagcatgatc atcccgtagc ggttagccga
     1921 ctcacggcgg aactcgtcgg aggtcgttct gcgcgtgagc aggaagcgat cccagccatg
     1981 atcgggcgtg aagacgggga gcgcatcgat tcccgacgag gcgtaacggg cgctgacgat
     2041 cgcgctggcg acgatccagc cgagcgcgta ggccaggcga tcgggtgtcg ccaggtaggc
     2101 ggtcgtgtca ggcatgtcgt cgctgaacgg ggcaggagag agtccttcgt ggttcgcgat
     2161 cgcacgctgg accaactcgc ggacgttcat cagttcggca cctcatgctt ctaacgtctt
     2221 cgaactcaac ctattgactg tagcaaacag cgcgacatcg ctgcatgaac cggcatgtcg
     2281 tcaggttccc tgctaagata cccgccagaa taacggccac aatcgaaaga agcatgcatg
     2341 actgagggcg atcggagcca gcgagtcctg ggaatctatc aaggttgggt tgtcgtcatc
     2401 gccgtcatgt tgacgctggc gatcgcctcg ggcggcaggt tcctgttcgg tgtcgtcctg
     2461 atcccggtta ccgaggaatt cggctggagc cgcgcggaac tgacgcttgc ggtcaccatc
     2521 agcgtcatcg cgctctcgat cctgcaaccg atcatcggcc tgctggtcga ccgcttcggg
     2581 tcacggcgca tcctgatcgc cggtgtcgtg acgacggcca tcgcgatgat cccgatcacg
     2641 atcgcgacca gcctctggca gatctatgtc ttctacggca ttctggccgc cttcggcttc
     2701 gccgcgacat cgcccgtcaa cacgaccgcg ctcgtcaacg gctggttcga gaagaagcgc
     2761 gggctggcgc tctcgatggc gacggccggc tcggcctatg ggcaattgct catcatcccc
     2821 atcgcaaccg ccatcatggt ccaatggggc tggcgcgcga gctactatgc cattacagcg
     2881 gcactggtcc tcctcgtcct gccgttggcg ctcttcttca ttcgggactc gccgtcgcgg
     2941 accgcatccc gccactcccg ccagaccggc gtctttgatg ggccgagagt cagtcttggg
     3001 caggcagtca ggaccgccgc atactggcaa atctcgttcg gcatgttcgt ctgcggcgcg
     3061 acgatgggct tcacgtcggt ccacatgatc ccatatctga ccgacatgcc ggagcactcg
     3121 acccacacga tgcagacgac ggcctcgctg gcgatgtcgg tcgtcggtgg cgccagcatc
     3181 gttggctcgc tggtgctcgg ctacctctct gaccgcaaag ggcaccgcca gatcctggcg
     3241 cttacctact tcctgcgcgg tctctcgttc ctggtcctgt tggcagttgg ttcgttcatc
     3301 cccggcatct tcatcgccgc gattatcctc ggcgtctcgt ggacatcgac gacgccgctc
     3361 ggttcggcaa tcgccgccga catcttcggc cgcgcttcga tgggcacgat cttcggcttc
     3421 atcttcacgt cgatgaacat cggctccggc ctcggcgcgt tcatcgccgg actggaccag
     3481 gatgtgactg gcaactacca cgccgtcctg gtcgccaacg tcgcactcgg gttcctggca
     3541 gcgatcgcga cctggtcgat caaggtccag ccatacgagg tcccgcagac cagcgtcctg
     3601 cgcgaccggg ccgaaccggc gctcacccgc agcattggcg actgagtgtc acgggcggct
     3661 caggccgtct cagccaggaa cgacaacacc tcgtcgagga actgccgccg gccgtactcg
     3721 ggccggtcga ggtgggcgaa gtgggtggcg tttaggagtg tcaccgttcg gaccgacgca
     3781 gcatcgatca gctgctccgc cagccgctcg acatcctccg gccgcgacca gaaatcgcgt
     3841 tcggaccgga tcaccagcgt gtgtgcccgg acaaacgacg catcccagag ctggcggccg
     3901 gtggccagat agaagctatc ttcgagagcg cctgtcggat gcgggaaggc cgtcgcgtca
     3961 ggatcgccgg tcgcggcctc gatcgccgcc cgcgcgtagg cggcagcaac ggccggatcc
     4021 cgccagcgat cgagttcccc ctctggaatc gagttgtccc acgtccgcag caacgaggcg
     4081 gcagcagaag atcgccagcc gcccgtcccg gcgcggtcga atcggccagg gttggcggcg
     4141 tcttccatcc cactgccacg tccgaggctg ggatggtcga acgtgctgcc gtagagcgtg
     4201 ttgaagatga tgagctgctt cacctggtcg ggatgcaggc tggcgtagaa gccggcccag
     4261 tgcccgcctg tcgcccagcc gagcagcgac acacgttcct cgccggtcac cgcgcgaacc
     4321 cagtcgacga cggcgtccag atcgcggacc acatcgacgg aacgaaccaa cggcgcgctc
     4381 gctgtggcag gctgctccat ctccggcggg cgtgacgagc cgccgtaccc gcgagcgtcc
     4441 atcacgaaca cgtcatagcc ggctccggcc aggtcagccg caagcgaacc gccctccacc
     4501 ggcacgtcga acgatgcaac gccgccggcc cgcgcgccgt gaaccaggat cagcggtgtg
     4561 gcagcgctcg ttgcgccgag cgcccagaca cgccggacgg ccagtgtcat gccgtctcgg
     4621 ccctcgatcc ggtgatcatc acgttgaatg gttcccatag gcagcttcct tgtcatctca
     4681 acggctgcat cgacagccaa agattatcca acaagccgct cctgacgtcg ttcgtgccgc
     4741 cgcatcagcg gagtcatcga gattccatgg acgacgatcg agacggcaac gacactgagc
     4801 gtcatcgaag ccagctgttc agcatctgga accgatagtc catgctcaac tgcgtaggca
     4861 agatagtaga tcgagccaac gccgcgcatc ccgaaccagc caagcagccg gcgttcgggc
     4921 tgcgacactc cacttccaac cagcgaaaac aacaccgaga caggtcgaat gactagaaac
     4981 agtagcggga tgaaccagat cgc
//
LOCUS       DASUGJ010000535         5862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_84261, whole genome shotgun sequence.
ACCESSION   DASUGJ010000535 DASUGJ010000000
VERSION     DASUGJ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84261"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2578
                     /locus_tag="VFV93_12100"
     CDS             <1..2578
                     /locus_tag="VFV93_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166132.1"
                     /translation="TPPYGPWTEILRGWPEGNGFPAVPDALRGGEAMAGLASQAALFE
                     LAADLLATAGAVRPIVLLLEDLHWADQPSLDLLRYLARWVAGFRIMLVATYRDDEITR
                     RHPLFALIPTLAREPGAERISLQRLAPDAVRDLIARHYSLEGDERERLATYVHRVTEG
                     NPFFAGEVLRALEESRAIEQVDGIWRLGDLDRVHVPELVRQVIEGRLARLGDEANRLL
                     GVAAVIGHEVPVDLWIAVSGEDEDLLSETLERATEARIVEELPRGNGFRFTHALIRET
                     LYEGLGLLRRRTLHRRAGEALAAGTRPDPDAVAHHFRQAGDERALDWLIQAAERANRS
                     YAWLVAAHQYEQAAEIIADVPDRQREYGWLLVHAAQMKRFYDPQLAVLYMEDAARIGR
                     DVDDVWLAMFPRMMAGFYRVMNGQMRAGVDQMVAVGPELEAIFRDGRLASWNLPALPG
                     AAVNPDVGYLTQVAAAHPDRPVVLGRSLTALHLAGVGRYREAQHLGEQLAAVWPPTII
                     TTDEVGFPAGMQHSFADLYLALAFVYYALGEPQRAIESSQIALSVYEKAAHHLLVAIS
                     YESIAKTLTTYSTEDNERRREAALAAADAIGRAQSALPTNAPTHMWSLPLAVVEGGWE
                     AVSTLLHDQVFGATATVAMSRQLAAAGLATISMHQGRYDVVHRVMRALFPDGIHTEPG
                     NLDFQAAIELQRVAIGLLLTSGDLDAARERLELYDRWMAWSGAILGQVEGHLGWARYH
                     RQAGDIAAAEEQARTALAKASAPRQPLGLVASHRLLGELATERGAYGDARDHLDQAMA
                     LADACAAPYERALTLIALAELDIATGADAGKTLAEARAICERLEARPMLERITALEAR
                     LP"
     gene            2575..5775
                     /locus_tag="VFV93_12105"
     CDS             2575..5775
                     /locus_tag="VFV93_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX5166133.1"
                     /translation="MSSQPQVFISYQRADEGFARQVRTHLAAHGVRTWMDQYDIPVGA
                     YWPDEIDKGLSSSDIVVGVLSPDSVESRNVKNEWDWAIQHDKRLVLLQVRPCDIPHRY
                     ISINFIDSTGPDQAPAFGALLQSLGVSAALVDPPDSSAGAGDAPIPSRHGVGRGVRRS
                     RAEPFVVGREREQELLTRQIEMLLAGQGSIVLVGGEAGIGKTTLTTWFAWAAEQRDAI
                     VCVGGCYDLTTTPPYGPWVEITRAWPADAHGFPSVPPELRGGDALARVHSQAALFEIA
                     SEFFITASAVKPIVLLLEDMHWTDQGSLDLLRYLARLVAGRRLMLIVTYRDDELTRRH
                     PLQQMLPSLIRESNAQRLGLQRLDDDAIETLISRQYPLPDSDLERLATYIEGVTEGNP
                     FFVTEVLHALEEAGTLSRETDGWGVSGLDQVRVPELVRQVTESRLDRLGGAARQLLEV
                     AAVIGHEVDIDLWIEIAGRDDTQLVEVLERAIEARLVEELPGGTRLRFSHALVRETLY
                     AGLVSIRRRAWHRQIGEVLSSKSRPDPDVIATHFQRAADPRASQWLVRAGERAQQAYA
                     WSTAVERYEAALEALSVSDSDPRERGWLQYRIARLQRFHYPRESLEYLDEALRVAAET
                     DDRALAAAARYSNGLCRFFAGEYPAAITDMSAGADMLEALPVVEQERLDLGPDPQGIP
                     TITNPRGLLVAAMTHVGYLNDALRMGEATREATPKSTALAELGWSHYGDRHGALAIAY
                     ALLGRVDEARSSFERARRIYRENGHFSTQAVTAYNQLRFASLPYRTDCLEEHAQLVAE
                     GTAAFARSAATVGFGDGPDAMSLPLLYLDGRWDAAVTYACSQFDADTFSALDRDISHL
                     TLALIALARGDVDECRRHIRVLLPAGAATEPGSVGLYDTLVMQRVLIALALDGGDREL
                     TASWLATQDTWLTWSGAVLGRAEYQLLLARYHLLAGDEDAALFAATTALEHASQPRQP
                     LALIAAHRTLGELLTRCVQLDQAAEHLHESMSLAESCAAPFERALTLTALAELDIATN
                     RDPREQLAAARAICERLEARPTLERITALEASSSIG"
BASE COUNT          983 a         1800 c         1991 g         1088 t
ORIGIN      
        1 gacgccaccc tacgggccgt ggacggagat cctgcgcggc tggccggagg ggaacggctt
       61 cccggcggtt cccgacgcgc tgcgcggcgg cgaggcgatg gcgggcctcg ccagtcaggc
      121 ggcgttgttc gagctggcgg ccgacctgct ggcgacggcc ggcgcggtcc ggccaatcgt
      181 cctgctgctg gaagacctgc attgggccga tcagccatcg ctcgatctgc tgcgctacct
      241 cgcgcgatgg gtggccgggt tcaggatcat gctcgtcgcc acctaccgcg acgatgagat
      301 cacccggcgg cacccgctgt tcgcgctgat cccgacgctg gcacgcgagc ctggcgcgga
      361 gcgcatcagc ctccagcggc tcgccccgga tgccgtccgc gatctgatcg ctcgtcacta
      421 cagtctcgaa ggagatgagc gcgagcggct ggccacgtac gtccaccggg tgacggaggg
      481 caatccgttc ttcgccggcg aggtgttgcg tgccctcgaa gagtcgcggg cgatcgaaca
      541 ggtcgacgga atctggcgcc tgggcgacct tgaccgggtc cacgtgccgg aactcgtcag
      601 gcaagtcatc gaggggcggc tggcgcggct cggcgatgag gccaaccggc tactgggcgt
      661 cgcagccgtg attggccacg aggttccagt cgatctctgg atcgcggtga gcggcgaaga
      721 tgaagatctg ctttcggaga cactcgaacg ggcgaccgag gcccggatcg tcgaagaact
      781 gccgcgcgga aacggcttcc gcttcacaca cgcgctgatc cgggaaacgc tctacgaagg
      841 gttgggcctc ttgcgtcgcc gcaccctcca ccggcgggcc ggcgaggcgc tggctgccgg
      901 aacccggcct gaccccgacg ccgtcgcgca ccacttccgc caggcgggtg atgagcgggc
      961 gctggactgg ctgatccagg ctgccgagcg cgcaaaccga tcgtacgcct ggctggttgc
     1021 cgcccatcag tatgagcagg cggccgagat catcgccgat gtaccagacc gccagcgtga
     1081 atacggctgg ctgcttgttc atgctgcaca gatgaagcgg ttttatgatc cacaactggc
     1141 agttctatac atggaagacg cagcgcggat tgggcgcgat gtcgacgatg tctggctggc
     1201 gatgttcccc aggatgatgg ctggcttcta tcgcgtcatg aacggccaga tgcgggccgg
     1261 tgtggatcag atggtcgcgg tcggtcctga gcttgaggcg atcttccgcg atggtcggtt
     1321 ggcgtcatgg aatctgccag cgctaccggg tgcagcagtc aatccggacg ttggatattt
     1381 gacgcaggtt gccgcagcgc atccagaccg ccctgtcgtg ttgggtcgta gtctgactgc
     1441 gctgcacctg gccggagtcg gcagataccg tgaagcgcag caccttggcg agcagcttgc
     1501 agcggtctgg cccccaacca tcataacgac tgacgaagtt ggatttcctg ccggaatgca
     1561 gcattcattc gctgatctgt acctcgcact cgcatttgtg tactacgcgc tcggtgagcc
     1621 gcaacgtgcc atcgaatcgt ctcagattgc cctcagtgtt tacgagaaag cggcacacca
     1681 ccttctggtc gcaatcagtt acgagtccat cgcgaaaacg ctgaccacgt attccacaga
     1741 ggacaatgag cgccggcgag aagccgcgct tgccgccgct gacgcgatcg gccgagctca
     1801 aagtgccttg cccaccaatg ctccaacaca catgtggtcg ctgcctctgg cggttgttga
     1861 gggtggctgg gaggcagtct ctacgctatt gcatgaccag gtgttcgggg ccactgctac
     1921 ggtggctatg agcagacaac tggccgcagc cggtcttgcc acgatctcga tgcaccaggg
     1981 ccggtatgac gtggttcatc gggtcatgag agcgctattt ccggacggaa tacacacgga
     2041 gcctggcaac ttggactttc aagcagcgat cgagttgcag cgagttgcca tcgggctcct
     2101 gctgaccagt ggagatttgg atgcggcacg tgagcggctc gaactctacg atcgttggat
     2161 ggcgtggagc ggcgctatcc tcggccaagt cgaaggacat ctcggctggg cgcgctacca
     2221 ccggcaggcg ggcgatatcg ctgctgccga ggagcaggcg agaaccgcgt tagcgaaagc
     2281 gtctgcgccg cgccagccgc tcggtctcgt cgccagccat cggctgctgg gcgaactggc
     2341 gaccgagcgt ggcgcgtacg gcgacgctcg cgatcatctg gatcaagcga tggcactcgc
     2401 tgacgcctgc gccgcgccgt atgaacgggc gctaaccctc atcgcactgg cggaacttga
     2461 catcgcgacc ggcgctgacg caggtaagac gttggccgag gcgcgcgcca tttgcgaacg
     2521 gctggaggcg cggccgatgc tggagcggat cacggcgctg gaggcacgac tgccatgagc
     2581 agccaaccgc aggtcttcat ctcctaccag cgtgccgacg aagggtttgc ccggcaggtg
     2641 cgcacgcatc tggctgccca cggcgtccgg acctggatgg atcagtacga catcccggtt
     2701 ggcgcgtact ggccggatga gatcgataag ggactctcat ccagcgacat cgttgtcggt
     2761 gtcctctcgc cggattcggt tgagagccgc aacgtcaaga acgagtggga ttgggcgatc
     2821 cagcacgaca agcggctggt gctgctacag gtcaggccgt gtgatatccc gcaccgctat
     2881 atctcgatca acttcatcga ctcgactggc ccggatcagg ctccagcgtt cggcgcgctg
     2941 ctgcaatcgc ttggcgtttc agccgcgctc gtggatcctc cggattcctc ggcaggagca
     3001 ggtgacgcgc cgatcccgtc ccggcatggg gtgggccgtg gagtgcgacg gagccgggcc
     3061 gagccgtttg tcgtcgggcg cgagcgtgag caggagctgc tcacgcggca gatcgagatg
     3121 ctgctggcgg ggcagggttc aatcgtgctg gttggcggcg aagccgggat cggtaagacg
     3181 acgctgacca cgtggttcgc ctgggcggct gagcagcgtg acgccatcgt ctgcgtcggt
     3241 gggtgctatg acctgacgac gacgccaccg tatggcccgt gggttgagat cacgcgtgcc
     3301 tggccggcgg atgcgcacgg cttcccgtca gtccctcccg aactgcgcgg cggggatgct
     3361 ctggcgcggg tgcatagtca ggcggccctg ttcgagatcg cctccgagtt cttcattact
     3421 gccagcgccg tcaagccgat cgtgttgctg ctcgaagaca tgcattggac cgaccaggga
     3481 tcgctcgacc tgttgcgcta cctggcgcgc ttggttgcgg gacggcggct gatgctgatc
     3541 gtgacctacc gtgacgatga gctgacccgc cgtcatccgc tccagcagat gctgccgtcg
     3601 ttgattcgcg agtcgaacgc gcaacggctg gggcttcagc gcctcgatga tgatgcgatc
     3661 gagacgctga tctccaggca gtatccgctg cccgattccg atctggaacg actcgcgact
     3721 tacatcgaag gcgtcactga gggcaacccg ttcttcgtga ccgaggtgct gcacgcgctc
     3781 gaagaggccg gaacgctgtc gcgggagacc gatggctggg gagtgtcggg gctggatcag
     3841 gttcgggttc ccgagctggt tcgccaggtg accgagagcc ggttggatcg gctcggcggt
     3901 gctgcacgcc agcttctcga agtcgcggct gttatcgggc acgaggtgga tatcgacctc
     3961 tggatcgaga ttgccggtcg cgacgacacg cagctggttg aagtccttga gcgcgcgatc
     4021 gaggcgcggc tggtcgagga gctacctggg ggcactcggc tgcgcttcag tcacgcactg
     4081 gtgcgcgaga cgctgtatgc gggtctggtc tcgatccggc gtcgcgcctg gcatcgtcag
     4141 atcggagaag tgctttcgag caagtcacgg cctgacccgg atgtcatcgc gacgcacttt
     4201 cagcgggcgg ctgacccacg agcgagccag tggttagtgc gggccgggga acgagcgcaa
     4261 caagcctacg cctggagcac ggcggtcgaa cggtatgagg ctgccctgga agcgctgtcc
     4321 gtttccgaca gcgatccacg tgagcggggc tggctgcagt accggattgc ccggttgcag
     4381 cgctttcact acccgcgcga gtcacttgag tacctggacg aggcgctcag ggtggcggct
     4441 gagacggatg accgtgcgct ggccgctgcc gcgcgctaca gtaacgggct gtgccgattc
     4501 tttgccggtg agtatccagc ggcaatcacc gatatgtcgg ctggtgctga catgctcgaa
     4561 gcgttgccag ttgtggaaca ggagcggctt gacctcggtc cagatccgca gggcattcca
     4621 acgatcacca atccccgtgg actcctggtg gcagctatga cccacgttgg ttacctgaac
     4681 gatgcgttgc gaatgggtga ggcgactcgg gaggcgacgc cgaaatcgac cgcgctcgcg
     4741 gaactcggct ggtcgcacta tggcgatcgg catggtgcgc tggcgatcgc ctatgcgttg
     4801 ctcgggcgcg ttgacgaggc gcgctccagc tttgaacgcg ctcgccgtat ctaccgcgag
     4861 aacgggcact tctcgacgca ggccgtcacc gcatataacc agctccgatt cgcctcgtta
     4921 ccctatcgga cggactgtct ggaagaacac gcgcagctcg tggccgaagg caccgctgcg
     4981 ttcgcccgat ctgccgcgac cgtcgggttc ggtgacgggc cagacgccat gtcgcttcca
     5041 ctgctgtatc tggatggacg ctgggacgcc gctgttacct acgcctgctc acaattcgac
     5101 gctgacacct tcagtgccct cgatcgcgat atctcccatt tgacactggc actgatcgca
     5161 ctggcacgtg gagacgtgga tgaatgccgg cgacatattc gcgtccttct gccggctggg
     5221 gcagcgactg agccgggatc ggtgggtctc tatgacacac tggtgatgca acgcgtgctg
     5281 atcgcgctcg cactcgacgg cggtgatcgt gaacttacgg ccagctggct cgcgacacag
     5341 gatacgtggc tgacgtggag cggggccgtg ctcggtcgcg ctgaatacca gttactgctg
     5401 gcgcgctatc acctgctggc cggggacgag gacgccgccc tgtttgcggc aacgaccgcg
     5461 ctcgaacacg cgtcccagcc gcgacagccg ctcgcgctga ttgccgctca tcggacgctg
     5521 ggtgaactcc tgacccgttg cgtccaactg gatcaggcgg cggaacatct ccacgagtcc
     5581 atgagtctgg ccgagtcctg cgccgctccg ttcgagcggg cgttgacgct cactgcgctg
     5641 gccgagcttg atatcgcaac gaaccgcgat cctcgcgagc aactggccgc ggcgcgggct
     5701 atttgcgaac ggctggaggc gcggccgacg ttggagcgga tcaccgcgct ggaagcgtcg
     5761 tcgtcaatcg gctgacacgt ccgtatcgac gctggcagca ggtgcgggaa tcagcgatgt
     5821 gtctgctggg ccggctgcgc gaccgatggc gcgggcgatt tc
//
LOCUS       DASUGJ010000536         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1614566, whole genome shotgun sequence.
ACCESSION   DASUGJ010000536 DASUGJ010000000
VERSION     DASUGJ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614566"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..1594
                     /locus_tag="VFV93_12110"
     CDS             380..1594
                     /locus_tag="VFV93_12110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5166134.1"
                     /translation="MSCGILIVAPYLPWPADFGGAIRIYELVRYLAREQRVILLAPAS
                     PAEMHAIEHLSGICDVTVVPVEWTFRQTAGWRKRLTQARSLASRASFVELASAEPRFQ
                     AVMDRLFMTRDIDLVQYEFPQMTRFRPLQPRPTILDNHNIEHELLRRVAQTTQSLPQR
                     AFNNVEWRKVRRLERDAWLSVTLNLATSARDAGVISSVSGLEAITISNGVDMDAWSTA
                     RSTERTPGRVVFTGAMRHQPNADGVRWYAEQVHPLVLAAVPNATLEIVGADPPPSVAA
                     LATSSISVTGRVESVEPYLARASVAVVPLHAGGGTRLKILDAFAAGVPVVSTTIGAEG
                     LDVSPGRELLVADTPRAVADAVIGILNGRTSLTAPAALDFVRAHHDWTTAIGPGLVSA
                     HVAAIERFRSDG"
     gene            1688..2914
                     /locus_tag="VFV93_12115"
     CDS             1688..2914
                     /locus_tag="VFV93_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166135.1"
                     /translation="MRDADQRATRENVSSPLLPGGNLISASLDRQVMIWSDRGGASRH
                     IGDRWASRCSSALDDAIGTRWPVPLDEPFEVIHVIRLDDVPDVSREANRHHLENPDFL
                     LIGSRPSSTRPCLQAADAKFAADRIKPSQVSATIVQNLLAVEDGVTRALVEQTLADHG
                     LSEPEIVRGVFIAPQSELTDYLLRRVTTGKRASVDAREVVTIPPEPGAMFAGLPESRV
                     IGGLARIDALPVTPRDNLISAIYYFRLACACFYLWSEGNRPLLGGGPISEPEPGIVAA
                     EIATRSTAASSAYGLIDAWAVDVQPQIQARQAISDVATLPIRMGDIRQRLERAGSGEP
                     RAVRQVRRDLDIAFRARLAELVGVIEADDPRSLTQILSDVAGASRSLAGEMQTLLDQL
                     ISQAGWAGADNRSAAS"
BASE COUNT          538 a         1079 c         1030 g          548 t
ORIGIN      
        1 cggcaggaac tggacctcat cgggcttgta cgacttgccc cggtcgctga tcaggactgt
       61 gtcgccctcg accgacacgc cgtgttgcag catcccgtcg gccgcgaatc gcacctctcg
      121 catcccaggt tgttcccttc actgtgcggc gagcgccgcc ttcgatccga ccacccagca
      181 gactcccgtg caggcgggta tcgatcgatg aaggttggat agaatcaggc gttggtcgaa
      241 tgcatcacga cgagggaacg atgggaagac gaacccgcga tacatgcgtg gcaacgacga
      301 tcctgagacg ttccgcacca cccgctgcac aaacccattg tagaacacca acccgttggc
      361 tggttacgca ggcgacgcga tgagctgcgg catcctgatc gtcgcgccct acctgccctg
      421 gccggccgac ttcggcggag cgattcgcat ctacgaactc gttcgctatc tcgcgcgcga
      481 gcagcgcgtc atcctcctgg cgccggcgtc gcctgccgag atgcatgcga tcgaacattt
      541 gagcggcatc tgcgatgtca cggtcgtgcc ggtcgagtgg accttccggc agacggctgg
      601 ttggcgcaag cggttgacgc aggcacgttc gctggcctcg cgggcgtcgt tcgtcgagtt
      661 ggcgtccgct gaaccgcgct tccaggcggt gatggaccgc ctgttcatga cgcgcgacat
      721 cgatctcgtc cagtacgagt tcccgcagat gacgcgcttc cggccactcc agccacgtcc
      781 aaccatcctc gacaaccaca acatcgaaca cgagctgttg agacgcgtcg cccagaccac
      841 gcaatcgctg ccgcagcgcg cgttcaacaa cgtcgagtgg cgcaaggtgc gccggctgga
      901 gcgcgatgcc tggttatcgg tgacattgaa tcttgcgacc tcggcgcgag acgccggcgt
      961 catttcgagc gtgtctggct tggaggcgat caccatctcc aacggtgtcg acatggacgc
     1021 gtggtctacc gccagatcga cggaacgcac gccggggcgg gtcgtgttca ccggcgcaat
     1081 gcgtcatcag ccgaatgccg acggggtgcg ctggtatgcc gagcaggtcc acccactggt
     1141 gctggcggcc gttccgaacg caacgttgga gatcgtcggt gccgatccgc caccgagtgt
     1201 ggccgcgctg gcaaccagct cgatcagcgt gaccggccgc gtcgagagcg tcgagccgta
     1261 cctcgcgcgg gcctcagttg ccgtcgtgcc gctgcatgcc ggcggtggga cgcggctgaa
     1321 gatccttgat gcatttgcgg ccggtgtccc tgtcgtttcg acaacgatcg gcgctgaggg
     1381 gctggacgtg tctcccggca gggaactcct ggttgccgac accccgcgcg ccgtcgcaga
     1441 cgccgtcatc ggaatcctca acggacgtac gtcgctgaca gcacctgccg cactcgactt
     1501 cgtacgagca caccacgact ggacgactgc gatcgggccg gggctggttt cagcccacgt
     1561 ggcggcaatc gaacgctttc gcagcgacgg ctgatggagc gtctgctgta ccatgtaagg
     1621 gagaatagcc gcccagcagg gcgacagtgc gaaacgatgg tgatggctac gatgaaccag
     1681 gttggtcatg cgcgacgctg accagcgcgc aacacgggag aacgtcagtt cgccgctgct
     1741 tcccggcggc aacctcatca gtgcttcact cgaccggcag gtcatgatct ggagcgatcg
     1801 cggcggtgcc agccggcaca tcggcgaccg ctgggccagc cgctgctcca gcgcactcga
     1861 cgacgccatt ggaacgcgct ggccggtccc gctcgacgag ccgtttgagg tcatccatgt
     1921 gatccgcctc gacgacgtac ccgacgtctc gcgggaggcc aaccgccacc accttgagaa
     1981 tcccgacttc ctgctgatcg ggtctcggcc ttcctcgacc aggccgtgcc tgcaagccgc
     2041 cgacgcgaag ttcgcggccg accgaatcaa gccaagccag gtcagcgcca cgatcgtcca
     2101 gaacctgctg gccgtcgaag acggtgtcac ccgtgcgctg gtcgaacaga cactggcgga
     2161 ccatgggctg tccgagccgg agatcgtgcg cggcgtcttc atcgcgccac agtccgagtt
     2221 gaccgactac ctgctccggc gcgtcacgac cggcaagcgg gccagtgtcg atgcccgcga
     2281 ggtcgtcacg atcccaccgg agccgggcgc gatgttcgcg ggactgcccg agagccgggt
     2341 catcggcggg ctggcgcgga tcgacgcgtt gccggtgaca ccacgcgaca acctgatctc
     2401 cgcgatctac tacttccggc tggcctgtgc ctgcttctac ctctggagcg aaggcaaccg
     2461 gccgttgctg ggaggcggcc cgatctcgga accggaacca ggtatcgtgg ccgccgagat
     2521 cgccacccgc tcgacggcgg cctccagcgc ctacgggctc atcgacgcct gggccgtcga
     2581 tgtccagcca cagatccagg cccgtcaggc gatcagcgac gtcgcgacgt taccgatccg
     2641 aatgggcgac atccgccagc gcctggaacg agccggctcg ggcgagccgc gcgccgtccg
     2701 tcaggtgcgc cgcgatctcg atatcgcctt ccgcgcccgg ctcgccgaac tggtcggtgt
     2761 gatcgaggcc gatgatccac gctcattgac gcagatcctc agcgatgtcg ccggggcgtc
     2821 ccgctcgctg gccggcgaga tgcagacgct gctcgaccag ctcatcagcc aggccggatg
     2881 ggccggcgcc gacaaccgca gcgccgcgtc ctgacctgtc gtactgagaa gtccaccacc
     2941 gtgatgctgt cggtttgaac cggtgcgttc ccccgtcgtc ctgagccgct gcgaagagcc
     3001 tccccgcgtc aacttggtgc ggtgctacag tggagaggtc cttcgctgcg gctcagtagt
     3061 ccaccacagg gattatgccg ggttggaggt cgcttgcgcg ggataacagc gttccaacca
     3121 gcgtcgggcg tccttggtgg tacagcgcca gtgcacggtg gcctgggcgg cgttgcgttg
     3181 cgcgacccag gcatc
//
LOCUS       DASUGJ010000537         7318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1890575, whole genome shotgun sequence.
ACCESSION   DASUGJ010000537 DASUGJ010000000
VERSION     DASUGJ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890575"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VFV93_12120"
     CDS             complement(<1..707)
                     /locus_tag="VFV93_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5166136.1"
                     /translation="MTGGQALVASLRQYGLDTIFGLPGVQLDWAFDALYDARDDIRVY
                     NTRHEQATSYMADGYARVTGRVGACLVVPGPGLMNALAGLSTAYATNSPVLCIAGQID
                     SQQIGAARGMLHEIPDQLTMVRSATKWAGRAMSPQDVPALVRDAFTQLQTGRPRPVEI
                     EIPPDVLQATANVALLDPVTPERPSGDPDLLEQAAQALGQAQQPLIMAGGGVAGANAS
                     AALHELAEMLQAPVVVS"
     gene            complement(749..1345)
                     /locus_tag="VFV93_12125"
     CDS             complement(749..1345)
                     /locus_tag="VFV93_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166137.1"
                     /translation="MAEKQTITFSGENPGLTLYKPGTEQVIAAVSYWRCVYSEHGDGN
                     ATLIWVDPNESGLGDEAPHAIYTDNPGVGKLVAQRFTQHFGTFTDLGFGKMETTQARF
                     FQEGDGRWYHRVVTNTGDGVIELVWWDIIQHELVQRSNYEVGPVKWDLATVICPCKSA
                     TITINNKPVTGEVRWKDDGSGASSSAFLAFSETWRESR"
     gene            complement(1485..2930)
                     /locus_tag="VFV93_12130"
     CDS             complement(1485..2930)
                     /locus_tag="VFV93_12130"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5166138.1"
                     /translation="MQVGRVRHQGWFGGDARVAIARGARLALVAVFALSVMAGVARPN
                     AASAAESVVQIPMDENGMPTRTFFPETGHHVADEILNAWRSTGLMIFGYPISEPMVEN
                     GRTVQYFERARLEVWPEYKGTQWEVQGSLLGNWETEKRRNEPAFKRLPSNQPSDSPDR
                     VFFKETGHWLAYGFKKYWDANGGLWQFGYPISEEFQEKNAQDGQTYTVQYFERARFEY
                     HPEHAGTEFEVLLGHLGRDYAVAKKIDQKPVTQSANSVLYNEGLFDSNLLDAFRPHAG
                     SWMGFVSTDALGVRSEPATASPNVDVIYNRRPVEIHGIVRGTVVEGTDAWYIVGDNTY
                     VSAAYIDPLVLQAPPQTFGGHWVDVSLSSFYAVAYDGDTPVYVAIIAAGRDGKTPTGV
                     FQVQYQVRSEIMDSATVGIPPGAPGYYYLENVEYTQYFLAGGYALHGNYWTPEANFGG
                     FTSNGCVGLMNQDAAFFWDWMYPGDDISIHY"
     gene            3173..3475
                     /locus_tag="VFV93_12135"
     CDS             3173..3475
                     /locus_tag="VFV93_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166139.1"
                     /translation="MQIARDRSNGTLRQDALLALAETAGLFLIFIVAFTGRDAFGDIV
                     KLLLTILSIAVGGLGATGGLLVARRALAEPAGRVARLAIAGWMAFAGIYTIIHVLS"
     gene            3612..4958
                     /locus_tag="VFV93_12140"
     CDS             3612..4958
                     /locus_tag="VFV93_12140"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166140.1"
                     /translation="MKRKLGASRTLEASAVAPQQSASTTPSPITTEAPPAIETHSPSI
                     PAWKRPFTALEERDFRNLWVGMLPGTLAMQMGMVTTGWVAYDISESATSVGFVSLGSG
                     IPMLTLGLFGGVVADRFPKRRTLLMTQSLIGTAAAICAVLVLTGIVEIWHLMAISAMQ
                     GVGFAFNMPSRQAFVAQLISRERLMNAIALNNAGMNFARVIGPTLAGLFIAVIGPGEV
                     YVVMALMYAFVVYSLTRIPQSGAPVGNHRPPPLKSLADGLGYVRSNGVVFTLLVLAFA
                     PVLLGMPYQQLMPVFAEDVFGVGASGLGLLLSFNGIGALVGSLGIAGISNGFRRRGLL
                     QMATGITFGIAVAVFALSQSYPIALITLLFAGAASAAFQSLNSTLVMTNSDPAYHGRV
                     MSVYMLTFSAMPLGVVPFGALSDIYGAPITIGIGGILLTCVIAMVGLLHPSYRHIR"
     gene            5043..5639
                     /locus_tag="VFV93_12145"
     CDS             5043..5639
                     /locus_tag="VFV93_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5166141.1"
                     /translation="MDESALVERARSGDVAAFEQLVSRYQDLAVRTAWVVAGSAGEAE
                     DAAQEAFVKAYLALGRFRAGSPFRPWLLQIVVNEARNRRKSAGRRANLTLRAASSHHP
                     ETEPSPEATTLAVERQQELLDAVNRLRDEERLVVACRYFLGLSEAETADTLRCPRGTV
                     KSRLSRALDRLRNELSVTLGERGQPLDGSGDLEQTAHD"
     gene            5632..6450
                     /locus_tag="VFV93_12150"
     CDS             5632..6450
                     /locus_tag="VFV93_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166142.1"
                     /translation="MTEPQPTRPPDDLEQALTDLGVQLDWPAEPDLLPAVRQNLASRP
                     PRQRLFDRVMPLSTPRRVAWAAVTFVLIAALVLTISDSTRSTIADRLGLPGVSISTEP
                     TATAAPGAALSIGEPVTLDEAIARVGSGLLLPPTEALGAPDGVYMLDQDSVVQVTYVY
                     LPRPDLPEAGQSGVGLLISQFAGHTNQSFIQKQLGSGTTIEQVDVAGQPGFWLVGAPH
                     VFFYERPDGQIREESIRLAANVLLWEQDGQTLRIETALNRDAAIRIATAMVASH"
     gene            6536..7255
                     /locus_tag="VFV93_12155"
     CDS             6536..7255
                     /locus_tag="VFV93_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166143.1"
                     /translation="MRRLITSLVLALVAILAMASTAVAGGWATVEMDKPVTQIEVNEP
                     MTLDFTLLQHGVTPTDWTTTYVEARNNETGETLRFDAIAGSEVGRWTVDVTFPSAGTW
                     HWAIKTEELAVEDTFPAIEVVDSAGVISSSSGVTPAEMDAAITGATEPLEKQLSSMVG
                     QLDVLEKQVSNLSAERDTLQKQIANLETAQAEQPASSGTSWWVAALAGALAAVVVGGA
                     GLAFAARHGLIRTSELATASA"
BASE COUNT         1276 a         2426 c         2311 g         1305 t
ORIGIN      
        1 atcgagacga cgaccggcgc ctgcagcatc tccgccagct catgcaacgc cgccgatgcg
       61 tttgcgccag caacgccgcc accggccatg atcagcggct gctgagcctg acccagcgcc
      121 tgggcagcct gttcgaggag atccggatcg ccggagggtc gttccggagt gaccggatcg
      181 aggagcgcga cattggccgt cgcctggagc acatcgggcg ggatctcgat ttcaaccgga
      241 cgcgggcgac cggtttgaag ctgcgtgaac gcgtcgcgaa ccagcgccgg cacgtcctgc
      301 ggagacatcg cccggccagc ccacttcgtc gccgagcgca ccatcgtgag ttgatcgggg
      361 atctcgtgca gcatgccgcg cgctgcgccg atctgctggg agtcgatctg accggcaatg
      421 cagaggaccg gcgagttggt cgcatacgcc gtcgacaatc cggccagcgc gttcattagt
      481 cccggccccg gcacgacaag gcaggcacca acccgtcccg tgacgcgtgc gtacccgtca
      541 gccatgtacg aggtcgcctg ctcgtggcgg gtgttgtaga cgcggatgtc gtcacgcgcg
      601 tcgtacagcg catcgaatgc ccagtcgagc tggacaccgg gcagcccgaa gatggtgtcg
      661 agcccatact gtcggagcga ggcgacgagc gcctgccctc ccgtcatctc tggcacgtca
      721 gtttcctctc gatcgaccga tcactgcatc aacgagactc gcgccaggtc tccgagaagg
      781 cgaggaacgc tgaggagctg gcgccgctgc cgtcatcctt ccagcgaacc tcaccggtga
      841 ccggcttgtt gttgattgtg atcgtcgccg acttgcacgg gcaaatgacc gtcgcgagat
      901 cccacttgac cggcccgacc tcgtagttgc tacgctggac aagctcgtgc tggatgatgt
      961 cccaccagac gagttcaata acgccgtcgc cggtgttggt cacgacgcgg tgataccagc
     1021 ggccgtcgcc ctcctggaag aagcgcgcct gggtcgtctc catcttgccg aagccgaggt
     1081 ccgtgaacgt cccgaagtgc tgggtgaagc gctgggcaac cagcttgccg acgcctggat
     1141 tgtcggtgta gatcgcgtgc ggtgcctcat cgccgaggcc cgattcgttc ggatcgaccc
     1201 agatcagcgt cgcgttgccg tcgccgtgct cggaatagac acaacgccag tacgagacgg
     1261 ctgcaatgac ctgctccgtg cccggcttgt acagtgtcag cccgggattc tctcccgaaa
     1321 aggtgatggt ctgcttctct gccatcgtcc gcggccctcc ctgcgtctct tgatattcgt
     1381 caacagcacg attgtacggg caatgtcacc ggcgcggcgg tcaccggagg tacagtttcg
     1441 cctgcgaagc aggggccggc cttgtgccgg cccctgctct tagactagta gtggatgctg
     1501 atgtcgtcgc cggggtacat ccagtcccag aagaaagcgg cgtcctggtt catgaggccg
     1561 acgcagccat tggaggtgaa accgccgaag ttggcttctg gtgtccagta attgccgtgc
     1621 agggcgtagc cgccggccag gaagtactgc gtgtactcga cgttctcaag gtagtagtaa
     1681 cctggcgcgc cgggcgggat gccgaccgtg gccgagtcca tgatctccga gcgcacctgg
     1741 tactggacct ggaagacgcc ggtcggcgtt ttgccgtcgc gtccggcggc aatgatcgcg
     1801 acgtagacgg gtgtgtcgcc atcgtaggca acggcgtaga agctgctcag gctgacatcg
     1861 acccagtggc cgccgaacgt ctgcggcggc gcctggagga cgaggggatc gatgtaggcg
     1921 gccgagacat atgtgttgtc gccgacgatg taccaggcgt cggtcccctc gaccacggtt
     1981 ccgcgcacga tgccgtgaat ctcgactggc cggcggttgt agatcacgtc gacgttcgga
     2041 gacgcggtgg ccggctcgct gcggacgccg agcgcgtcgg tcgagacgaa gcccatccag
     2101 cttcctgcgt gtggacgaaa agcatccagc aggttgctgt cgaagagacc ttcgttgtag
     2161 agcacactgt tggccgactg ggtaacgggc ttctggtcga tcttcttcgc gacggcgtag
     2221 tcacgcccga ggtggccgag caggacctcg aactccgtcc cagcatgttc cgggtggtac
     2281 tcgaagcggg cacgctcgaa atactggacc gtgtaggtct ggccgtcctg agcgttcttc
     2341 tcctggaact cctcggagat cgggtagccg aactgccaga ggccgccatt tgcatcccag
     2401 tacttcttga acccgtatgc caaccagtgg ccggtctcct tgaagaagac gcggtcgggg
     2461 ctatcgctcg gctggttgga cggcaggcgc ttgaacgccg gttcgttgcg acgcttctca
     2521 gtctcccagt tgcccagcag cgagccctgg acctcccact gggttccctt gtactccggc
     2581 cagacttcaa gacgggcgcg ctcgaaatac tggaccgtcc ggccattctc gaccatcggc
     2641 tcggagatcg ggtagccgaa gatcatcagc cccgttgagc gccaggcatt caggatctca
     2701 tccgcgacat gatgtccggt ctccgggaag aacgtgcgtg tcggcatgcc gttctcgtcc
     2761 atcggaatct ggacgacact ctcggcggca ctcgcggcat tcggccgcgc cactcccgcc
     2821 atgacactca gcgcgaacac ggcaacgagc gccagccgcg ccccacgcgc gatggcgacc
     2881 cgagcgtcgc ctccgaacca accctgatga cgcacacgac cgacctgcac gaaaactccc
     2941 cccggacata ctgaaccagc gagacgccag acgtgcggca ccccgccata cccgatattc
     3001 ctacatcagg ttcaacgaac gacggacgac aacagttcca ccgacgatgc gtcgccggca
     3061 tccggcatca gacgttcgct cgcaagcagt acaattcaac cgcatcgcgc cccgcgtgaa
     3121 cacgccttcc agctaccaaa acttcgacaa tcgacgttaa gaggacggac aaatgcagat
     3181 cgcacgcgac cgatcgaatg gaacgctgcg ccaggacgcg ctcctggcgt tggcagagac
     3241 agccgggctg tttctgatct tcatcgtcgc cttcaccggc cgggacgcct tcggcgacat
     3301 cgtcaagctg ttgctgacga ttctgtcgat cgcagtcggt ggtttgggcg cgacaggcgg
     3361 attgcttgtc gcgcgtcgtg cgctggccga accggccggg cgtgtggccc ggctcgcgat
     3421 cgccggctgg atggcattcg ccggcatcta cacgattatt cacgtattgt cataatagcc
     3481 agttggcggc gtctgttgcg ccccaggacc gggacgcgta ttcttcgcca acacaatggc
     3541 atcgacgcta gccgccgggt ggatgccatc gagaaggggt atccatccgc gtgaatgctg
     3601 atcgtgcgtc ggtgaaacgc aaacttggcg caagcagaac gcttgaagcc agcgctgtcg
     3661 cgccgcagca gtccgcgtca acgactccct ctccgattac gaccgaagca cctcccgcaa
     3721 ttgaaaccca ttcgcccagc atccccgcct ggaagcggcc gttcacggcc ctcgaagaac
     3781 gcgactttcg caacctctgg gtcggcatgc tgccaggtac gctggcgatg caaatgggga
     3841 tggtgacaac cggctgggtc gcgtacgaca tctccgagtc ggccacgtcg gttggcttcg
     3901 tctcactggg gtccggtatc ccgatgctga cgctggggct gttcggcggc gtcgtcgccg
     3961 accgcttccc aaagcgacgc acgttgttga tgacgcaatc gctgatcgga acggcggccg
     4021 ccatctgcgc cgtcctggtt ctgaccggca tcgtcgagat ctggcacctg atggcgatca
     4081 gcgcgatgca gggtgttgga ttcgctttca acatgccctc ccgtcaggcg ttcgtcgccc
     4141 agctcatcag ccgcgagcga ctgatgaacg ccatcgcact taacaacgcc ggcatgaact
     4201 tcgcccgcgt catcggccca acgctggccg gcctcttcat tgccgtgatt gggccgggcg
     4261 aggtctatgt cgtgatggcg ctgatgtacg ccttcgtcgt ctacagtctg acgcgcatcc
     4321 cgcagtccgg cgcgccggtc ggcaaccacc gcccaccacc gctcaagtcg ctggccgacg
     4381 gcctcggcta cgtgcgttcg aacggcgtcg tcttcacgct gctggtgctg gccttcgcgc
     4441 cagtcctgct ggggatgccg taccagcagt tgatgccggt cttcgccgag gatgtcttcg
     4501 gcgtcggcgc gagcgggctg ggcctactgc tctctttcaa tgggatcggc gcgctggtcg
     4561 gctcgctggg gatcgccggg atcagcaacg gattccgccg gcgcggcctg ctccagatgg
     4621 cgaccggcat caccttcggg atcgccgtcg ccgtcttcgc cctgtcgcag tcgtatccga
     4681 tcgcgctgat cacgctgctc ttcgccggcg ccgcgtcggc cgccttccag tcgctcaaca
     4741 gcacgctggt gatgaccaat agtgacccgg cctaccacgg gcgcgtgatg agcgtctaca
     4801 tgttgacctt ctcggcgatg ccgctcggcg tcgtcccgtt cggcgcactc tccgacatct
     4861 acggcgcacc gatcacgatc gggatcggcg gcatcctgct gacctgcgtc atcgcgatgg
     4921 tcgggctgct gcacccttcg tatcgccaca tcaggtaacg tcgcacaccc gcacgccctc
     4981 ttcggaacct ttccggcaca accgctgtat aagggacgag aagcagcgtc tgggaggacc
     5041 gactggacga atcggcgctc gttgaacggg ccagatccgg cgatgtcgca gccttcgagc
     5101 aactcgtcag ccgctatcag gatctcgccg tccggacggc ctgggtcgtt gccgggtcag
     5161 ccggcgaagc cgaggacgcg gctcaggagg cgttcgtcaa ggcctatctc gcgctgggcc
     5221 gcttccgggc cggctctccc ttccggccgt ggttgttgca gatcgtcgtc aacgaagcgc
     5281 gcaaccggcg gaagtcggcc ggccggcgcg cgaatctgac actgcgtgcg gccagttcgc
     5341 atcacccaga aaccgagccg tcgccggaag caaccacgct ggcagtcgag cggcagcagg
     5401 agttgctgga cgccgtcaac cggctgcgcg acgaggaacg gctggtggtc gcctgtcgct
     5461 actttctcgg gctttccgaa gcggagacgg ccgacaccct gcgctgcccg cgcgggacgg
     5521 tgaagtcgcg cctgtcgcgc gccctcgatc gtctccggaa cgaattgagc gtcactcttg
     5581 gcgagcgtgg acagccgctg gatggatccg gcgatctgga gcagacggcg catgactgaa
     5641 ccgcaaccga cacgaccgcc cgacgacctc gaacaggcgc tgaccgacct cggcgtgcag
     5701 ctcgactggc ctgccgagcc ggatctgcta ccggccgtcc gccaaaacct cgcatcgcgt
     5761 ccaccgcgcc aaaggctttt cgaccgtgtc atgccgctct cgaccccgcg acgggtcgcc
     5821 tgggcggcgg tgacattcgt gttgattgct gccctggtcc tcaccatctc ggactcaacg
     5881 cgttcgacga tcgccgaccg gctgggactg ccgggtgtgt cgatctcgac cgagccgacc
     5941 gcgaccgctg cccccggcgc cgcactctcg atcggtgagc cggtgacact cgacgaggca
     6001 atcgcgcgcg tcggtagcgg actgctcctg ccgccaaccg aggcgctcgg cgctccggac
     6061 ggcgtctaca tgctcgacca ggacagcgtc gttcaggtga catacgtcta cctgccgcga
     6121 ccggacctcc ccgaggctgg tcagtccggt gttggactgc tcatcagcca gttcgccggc
     6181 cacacgaacc agagcttcat ccagaagcag ctcggctccg gcacgaccat tgaacaggtc
     6241 gatgtcgccg ggcagccggg cttctggctc gttggtgcgc cgcatgtctt cttctacgag
     6301 cgaccggacg gccagattcg cgaggagtcg atccggctgg ccgccaacgt tctgctctgg
     6361 gagcaggacg gccagacgct ccggatcgaa acggcgctca atcgcgacgc ggccatccgg
     6421 atcgcgacgg cgatggtcgc gagccactga cgaccccgtt tggaaccgat ccgggccggg
     6481 cgctgtacta cagccagaca cacgatgcgg tcatccacaa tgaacggagg gcacgatgcg
     6541 gcgcctgatc accagcctcg tcctggcact ggtcgccatc ctggcaatgg catcgacagc
     6601 ggtcgccggc ggctgggcga cggtcgagat ggacaaaccc gtcacgcaga tcgaggtcaa
     6661 cgagccgatg acgctcgatt tcacgttgct ccagcacggc gtgacaccaa ccgactggac
     6721 gacgacgtac gttgaggccc gcaacaacga aacgggcgaa acgctccgct ttgacgcgat
     6781 cgccggcagc gaagtcggcc gctggacagt tgatgtcacc ttcccgagcg cgggaacctg
     6841 gcactgggcg atcaagaccg aggaactcgc ggtcgaggac accttccctg ccatcgaggt
     6901 cgtcgatagc gcgggcgtca tcagcagctc gagcggtgtg acaccggccg aaatggacgc
     6961 cgctatcacc ggcgcgaccg agccgctgga gaagcaactc tccagcatgg tcggacaact
     7021 ggacgtgctc gagaagcagg tctcgaactt gtcagccgag cgcgacacgc ttcagaagca
     7081 gatcgccaac cttgagacgg cccaggccga acaacctgcc tcgtccggca caagctggtg
     7141 ggttgcggcg ctggctggcg cgctggccgc tgtcgtcgtc ggtggcgcag ggttggcatt
     7201 cgcggccaga cacggcctga tccgaacgtc ggaactggcg actgcgtcag cctgaagtgg
     7261 ccctgtcgtc ctgagccgca gcaaaggacc tctccgctat gacccagcac ggcgttag
//
LOCUS       DASUGJ010000538         3311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1473968, whole genome shotgun sequence.
ACCESSION   DASUGJ010000538 DASUGJ010000000
VERSION     DASUGJ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473968"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..318)
                     /locus_tag="VFV93_12160"
     CDS             complement(10..318)
                     /locus_tag="VFV93_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166144.1"
                     /translation="MAENTGAEAVAVTEDRVAEVTAKLMQWSETLPEAEQLALASILE
                     AAVGADDTRGHIIIVGGSPASLPDIIRGGVLRGIFESKVGPAANQPAGAPGSSVNLSE
                     "
     gene            complement(368..1099)
                     /locus_tag="VFV93_12165"
     CDS             complement(368..1099)
                     /locus_tag="VFV93_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166145.1"
                     /translation="MLAISMLAAVALGMLASGPAHAQEPEPGRAPTLATFRGSYNGWS
                     TPKVEGPVRLHAGLVVVRARHGGNANFGLTLVTADPGKAPEDSYENRYLMINAIGRYD
                     GAAAELLTEDGDYYLLIGAGGAYEFTIEQPMPSNVTPVDKRAFDGAGQQVTSVFALDA
                     GTYSINGTSNGDGNFRVWMYEVDDLGGGAVEGNVFGRMFDVTSGPANESATVTLRRKG
                     LYLFYVEAWKIAGPQGAADWTVRVD"
     gene            complement(1302..2177)
                     /locus_tag="VFV93_12170"
     CDS             complement(1302..2177)
                     /locus_tag="VFV93_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166146.1"
                     /translation="MRYLPTIRTVVRLAAAALVATWLAAGIGAGVANADDDVGAPEVQ
                     GLRLEAPVAYVTFKDNASGETSFLIILSERNDPNRVSAVWSTGGVPGVGRVATRQVDG
                     IRPGVAYCVVVQAFRSADGLEVLTDLSGLPGTGHSRESNAVCTDPDVRPPDLAMERIG
                     GREERDWATVAGQAPAYLVAFRNDGADARGTVTVDIATSGVASLGDQIAVARQGWEAA
                     GFTCASRRTTGAETAALRCTGGSLKQGERVNSAVIVRFTGPGYGYIHASISVSDGPVE
                     TDTSDNLAVLGVRVY"
     gene            2825..3070
                     /locus_tag="VFV93_12175"
     CDS             2825..3070
                     /locus_tag="VFV93_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEX5166147.1"
                     /translation="MKLTYDPRHNIAYLRLVEPRGATEVETIQVSDDLNVDVAPDGTV
                     FGIELMNANEQLGGEDDGLLIVINEALGLRRELSLSE"
     gene            3132..>3311
                     /locus_tag="VFV93_12180"
     CDS             3132..>3311
                     /locus_tag="VFV93_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166148.1"
                     /translation="MSIATWTTEDSDALYRVSGWGEPYFSIDPSGDVIVSPSGNGGPT
                     INLYQLVQDLQTRGLT"
BASE COUNT          611 a         1132 c          949 g          619 t
ORIGIN      
        1 cgccgtcgat cactcagaga gattgacgct cgatcctggc gcgccggccg gctggttcgc
       61 cgccggcccc accttggatt cgaagatgcc gcgaagcacg cctccgcgaa tgatgtccgg
      121 tagtgacgcc gggctgccac ctacgataat gatgtgccct cgcgtgtcat ctgccccgac
      181 ggcggcctca aggatgctcg ctagtgcaag ctgttccgcc tcgggcagcg tctccgacca
      241 ctgcatcagc ttcgccgtca cctcggcgac ccgatcctcg gttaccgcaa ccgcctcagc
      301 tccagtgttc tcagccatta tcgattcctt ccagccggct catcgatcgc ctgaacctag
      361 gcactcgtta gtcgacacga accgtccaat cggcggcgcc ctgcggacca gcaatcttcc
      421 acgcttcgac gtagaacaaa tagagcccct tccggcgcag cgtcactgtc gccgactcgt
      481 tcgcgggacc gctggtcacg tcaaacatcc ggccaaagac gttgccctcg accgcgccgc
      541 cgccgagatc gtccacttcg tacatccaca cgcggaagtt cccgtcaccg ttggacgtgc
      601 cattgatcga gtatgtaccc gcatccagcg cgaagaccga cgtcacctgc tgcccggcgc
      661 cgtcgaacgc ccgcttgtcc accggcgtaa cgttgctggg catcggctgc tcgatcgtaa
      721 actcataggc gccgcccgcg ccgatcagca ggtagtagtc gccgtcttcg gtcagcaact
      781 cggccgccgc tccgtcgtag cggccgatcg cgttgatcat caggtagcga ttctcgtagc
      841 tgtcctccgg cgccttgccg ggatcggcgg ttaccagcgt cagcccgaag ttcgcgttgc
      901 cgccgtggcg ggcacgcacg accaccagcc ccgcgtgcag ccggaccggg ccttcgacct
      961 tcggcgtcga ccaaccgttg tagctgcccc ggaacgtcgc cagcgtcggc gcccggcccg
     1021 gctccggctc ctgcgcgtgc gccggccccg aagccagcat gcccagcgcg acggccgcaa
     1081 gcatgctgat cgccaacaca aagcggttaa ccctccatcg gaacgcactg cgtccatcgt
     1141 cacccctgat ccgttcactt ctccgagact tggataccgt catgttcgtt tcctttctcc
     1201 ggctcgattg cgtccgcctt tattcgctac cgcaaagaca ccaggacacg agcatccatt
     1261 tcggcaacgg agcccggcgt catcgcggta ggcacgtacc actagtagac cctgacgccg
     1321 agaactgcca gattgtcgct cgtgtccgtc tcgaccggac catcgctcac actgatcgac
     1381 gcgtggatat aaccatagcc aggacccgtg aatcgtacga taaccgccga gttcactcgc
     1441 tcgccctgtt tgaggctgcc gccggtgcag cgaagtgctg ctgtctctgc tcccgtcgtt
     1501 cgtctggacg cacaggtaaa gcccgctgcc tcccagccct ggcgggctac cgcaatctgg
     1561 tcgcccagcg acgccacgcc cgatgtcgcg atatcgaccg tcaccgtccc tcgtgcatcc
     1621 gccccgtcgt tgcgaaaggc caccagatag gcgggagcct gtccggccac tgtcgcccag
     1681 tctcgctcct cgcgtccgcc gatcctctcc attgccagat ccggcggccg tacatccggg
     1741 tcggtgcaga cagcgttcga ctcgcgcgag tgacccgtgc cgggcaatcc actcaagtcg
     1801 gtcaagacct caaggccatc agctgatcgg aatgcctgga cgaccacgca ataagcgact
     1861 cccggccgga tgccatcgac ctggcgcgtg gccactcggc ccacgccggg cacgccgccg
     1921 gtcgaccaga cggcactgac tcgattgggg tcgtttcgct cactcaggat gatcaagaat
     1981 gacgtctcgc cgctcgcgtt gtccttgaac gtaacgtagg cgacgggcgc ctcaagccga
     2041 agcccctgga cttccggcgc gcccacatcg tcgtcggcat tggcaacgcc ggcgccgatt
     2101 cccgccgcca gccacgtcgc tacgagagcc gcggcggcca atctgaccac tgttcggatc
     2161 gttggtagat atcgcatcgg cggctccttc gtgcattcac tcggccgaac gtctgcatca
     2221 gcatgagtgt ccggaccatg tggcgctgta ctcgaagtga cacttcgcct gtaatcgagg
     2281 taacactcaa accgcgccgg cccgaagtcg aacctctcat tgccctagga cggaatatga
     2341 tcgatcagtc tgagacgctg ccaggcatgt caattagcgg tgaatgttac gatgatcgtt
     2401 ggcgcagccc tgtccaacta ttggctcgtg gaggagcctg catgtcgatc gccacctgga
     2461 ccgtcgaaga cagcgacgcg cgtaaccgca ccatcggcag gaaaggggct atctccggcg
     2521 actgccattc ggagcttgag ccggcgggat gattgtccgc ctgcactcgc atgcgcgtgc
     2581 ccgtctcagc gaacgcggcg tcgaggagtg ggaggccgtg agcacggtca acacgggggc
     2641 ccggatgccg gcgaagttcg gccgcacgag attccggcaa gactactcat tcggttctca
     2701 gtggcgcgga cggcactaca cgacgaagcg tgtcgatgta atcgccgtcg aagaggatga
     2761 gggttggctg attcttacgg taatcgcacg ctacttctag cgcgtacttc ttgggagtca
     2821 cgagatgaag ctcacctatg atccgcgtca caatatcgct taccttcgcc tcgtcgaacc
     2881 gagaggcgcg acggaagtgg agacaatcca ggtcagtgac gatctcaatg tggacgttgc
     2941 acccgacggt acggtattcg gaattgagtt gatgaacgca aacgaacagc ttggcggaga
     3001 ggatgatggc ctcctcatcg tcatcaatga agccctcggg ctgcgacggg aactttcgct
     3061 gtcggagtaa cgcatcgcac ggcgtttcag caaccacgcg ccaccatgtc gaacgccgac
     3121 gaggagcccg catgtcgatc gccacctgga ccaccgaaga cagtgacgcg ctctaccgtg
     3181 tcagcggttg gggcgagcca tactttagca tcgacccgag cggtgacgtc atcgtctcgc
     3241 ccagcggcaa cggtggtccg acgattaacc tctatcaact cgtccaggat ctgcagaccc
     3301 gcggtcttac t
//
LOCUS       DASUGJ010000539         4962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1614821, whole genome shotgun sequence.
ACCESSION   DASUGJ010000539 DASUGJ010000000
VERSION     DASUGJ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614821"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VFV93_12185"
     CDS             <1..1154
                     /locus_tag="VFV93_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX5166149.1"
                     /translation="RVETVPIASSIRNAFIDFSKMTASVVAVVTDVMRDGRRVVGYGF
                     NSNGRYAPTALLRERFIPRVMAAEPTSLLDEAGDNLDPHAIWQIVMTGEKPGGHGERS
                     VAVGALDMALWDAVAKIAGQPLHRLLAERYGTGTPDERVWVYAAGGYYQPGKGLDALQ
                     DEMRGYLDLGYTTVKMKIGGASLAEDLRRIEAVLEVVGGDGQRLAVDANGRFELATAI
                     AYAEALSPYNLRWYEEAGDPLDYALQAELALHYTPPLATGENLFSMQDARNLIRYGGM
                     RPGHDVLQFDPALSYGLVEYLRTLEMLREHGFSGRDCVPHGGHQMALSMAAGLGLGGN
                     ESYPGVFQPFGGFADTTPIEDGHVRLPEVPGVGFEEKSALYAVMREVAE"
     gene            1185..1754
                     /locus_tag="VFV93_12190"
     CDS             1185..1754
                     /locus_tag="VFV93_12190"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5166150.1"
                     /translation="MSEEYQVEPISVEEYLRLEETSEIRHEYVGGYLYAMTGASWRHN
                     KIVNNFNTRIFDAADDQGCLVGITEVKLQVSERVIYSPDLLVSCDPEDRDAYIARHPC
                     LVVEVLSPSTTRTDRREKFLAYLLIPTLRAYLIVHQDRHLVERNWRDNATDEWRTQLI
                     SGGVVPLPCPDVELTVEEIYRNLPPGADV"
     gene            complement(1755..3224)
                     /gene="dnaA"
                     /locus_tag="VFV93_12195"
     CDS             complement(1755..3224)
                     /gene="dnaA"
                     /locus_tag="VFV93_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX5166151.1"
                     /translation="MSGQVMADDHAAASSQQTALRYEQLWQTALGDLQRQVSRANYET
                     WLRSTSLVGVAGETATVAAPNAFAVEQLRHKFDREITAAISALVNRSVTVEYVVRGAS
                     GEPAPRVPKPKPRPEPPAARTQRSEPSTSHVPHPASPQQMSLAPAERPGLNPGYTFES
                     FVVGPSNRLPHAAAMAVADKPGQAFNPLFIYGGVGLGKTHLLHAIGHRAIDQRPETQV
                     LYVSSEKFTNDLIRSIMNQRQTEFRDRYRSADILMIDDIQFIAGKESTQEEFFHTFND
                     LYQSGRQIIVCADKPPKAIATLEDRLRSRFEGGLIADVQSPDFETRTAILSRKGQSLG
                     IHVPGDVLEYVARKVQSNIRELEGALNKIIALAQLYNTSIRMEIAIQALNDAALEARR
                     AAITPDRVISEVTRQFKVSLTELRGRGRSKEIVLPRQIAMYTLREETGTSLVEIGSLL
                     GGRDHSTVMHGIAKIEKALETDTALRQKVSTLREALYEG"
     gene            3406..3975
                     /locus_tag="VFV93_12200"
     CDS             3406..3975
                     /locus_tag="VFV93_12200"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX5166152.1"
                     /translation="MNNVDGGDRPIGTYEYLDYSDPAAIFRPYDPRFPEVARRVAALI
                     EGRMPGARVEHVGSTAVPNCAGKGNIDLLLRYTAGELATAREALDSLGFQRQQGANPF
                     PESRPLRLGAIDYDGETFRLHVHVVGWDDPEAAELLRFRDRLRADPALVEAYVASKRA
                     ALTAGPTDNIAYNRAKQPFIRGVIAEERP"
     gene            4260..4937
                     /locus_tag="VFV93_12205"
     CDS             4260..4937
                     /locus_tag="VFV93_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166153.1"
                     /translation="MLRRLSTTLKSHQGVAMPDTADPNTPVTITLERWRWEQILLCLD
                     RYVAWLRGEYSDDDAMSDDVEWADELGWMTDDLRAIVNASGSHPVPPSTSFDALSPQL
                     GEEAATFVETPPPNSAPKRNPPWNSSENDAAIAAYFWMLGEQDAFRDFNKRAKYQELM
                     DGPLNRRSRKSIEFKMCNISAVFQRHGIEWVEGLAPLGNAQADLAVAVEEALAERGMI
                     DQTTDAR"
BASE COUNT          874 a         1531 c         1677 g          880 t
ORIGIN      
        1 ttcgggtcga gacggtgccg atcgcgtcgt cgattcggaa tgcgttcatc gacttcagca
       61 agatgacggc gtccgtcgtc gccgttgtga ccgatgtgat gcgcgacggg cggcgggtgg
      121 ttggctacgg gttcaactcg aacgggcgct atgcgccgac cgcgctgttg cgcgagcggt
      181 tcatcccacg ggtgatggcg gctgagccaa catcgctgct ggacgaggcg ggcgacaacc
      241 tcgacccgca cgcgatctgg caaatcgtga tgaccggcga gaagccgggc gggcatgggg
      301 agcggtcggt cgccgtcggc gcgctcgaca tggcgctctg ggacgcggtc gccaagatcg
      361 cgggccagcc gctccaccgg ctgctggctg agcgctacgg gactggcaca cccgacgaac
      421 gagtctgggt ctacgcggcg ggcggctact accagcctgg caagggcttg gacgcgctcc
      481 aggacgagat gcgcggctac ctcgacctcg gctacacaac cgtcaagatg aagatcggcg
      541 gcgcgtcgct ggccgaggac ctgcgccgga tcgaggccgt cctggaggtc gtcggcggcg
      601 acgggcagcg gctggccgtt gatgccaacg ggcgcttcga actggcaacg gcgatcgcct
      661 acgccgaggc gctgtcgccc tacaacctgc gctggtacga ggaggccggt gacccgctcg
      721 actacgcgct ccaggctgag ctggcgctcc actacacgcc accgttggcg accggcgaga
      781 acctgttctc gatgcaggac gcccgcaatc tgatccgcta cggcgggatg cgccccggtc
      841 acgacgtgct ccagttcgac ccggccctct cctatgggct ggtcgagtat ctgcgcacgc
      901 tggagatgct ccgcgagcac ggcttctcgg gccgcgactg cgtcccccac ggcggccacc
      961 agatggcgct ctcgatggcg gccggcctgg gcctcggcgg caacgagtca taccccggcg
     1021 tcttccagcc gttcggcggc ttcgccgaca cgacaccgat cgaggacggc cacgtccggc
     1081 tgccggaggt gccgggcgtc gggttcgaag agaagtcagc gttgtacgcg gtcatgcgag
     1141 aagtggcgga gtaagcgtca catgattggg cgtatactgt caccatgagc gaggaatatc
     1201 aggtcgaacc aatcagtgtc gaggaatatc ttcgactgga agagaccagc gaaatccgcc
     1261 acgaatacgt gggcggctat ctgtacgcga tgaccggtgc atcctggcgg cacaacaaga
     1321 tcgttaacaa cttcaacacc cggatattcg atgcagccga tgaccagggg tgtctcgtcg
     1381 gcatcactga agtcaagcta caagtgtcgg agcgcgtcat ctactctcct gacctgctcg
     1441 tctcttgcga tcccgaagac cgtgacgcct acattgctcg gcatccgtgt ctggtggttg
     1501 aggtactgtc tccaagtacg acaagaaccg accggcgaga gaagttcctc gcgtatctcc
     1561 tgatcccgac tctacgcgcg taccttatcg tccatcagga tcgacacctc gtcgaacgaa
     1621 actggcgcga caatgcaacc gatgagtggc gaactcaact catcagcggt ggcgtggtcc
     1681 cgttgccgtg tccggacgtc gaactgacgg tcgaggagat ttatcggaat ctcccgccgg
     1741 gcgcagacgt gtaatcatcc ctcgtacagc gcctcacgca gggtactgac cttctggcgt
     1801 aacgccgtgt cggtctccag cgccttttcg atcttggcga tgccgtgcat gacggtcgaa
     1861 tggtcgcggc cacctagcag gctaccgatc tcgacgaggg aggtgccggt ctcctcgcgc
     1921 agcgtgtaca tcgcgatctg gcgcgggagg acgatctctt tgctgcggcc acggccccgt
     1981 agctcggtca gcgagacctt gaactgcctg gtcacctcgc tgataacgcg gtcgggggtg
     2041 atcgcggccc ggcgcgcttc gagggcggcg tcgttgaggg cctggatcgc gatctccatc
     2101 cggatgctgg tgttgtagag ctgggccagg gcgatgatct tgttgagcgc gccctccagc
     2161 tcgcggatgt ttgactggac cttccgcgcg acgtactcca gcacgtcgcc ggggacatgg
     2221 atgccgagtg actggccctt gcgcgagagg atggcggtgc gcgtctcgaa atcgggcgac
     2281 tggacatcgg cgatcaggcc accctcgaag cgcgagcgga gacggtcctc cagcgtcgcg
     2341 atcgccttcg gcggcttgtc cgcacagacg atgatctgcc ggccggattg atacaggtcg
     2401 ttgaaggtgt ggaagaactc ctcctgggtc gattccttgc cggcgatgaa ctggatgtcg
     2461 tcgatcatca ggatgtcggc cgagcggtag cggtcgcgga actcggtctg gcgctggttc
     2521 atgatcgagc ggatcaggtc gttggtgaac ttctccgacg agacgtagag cacctgcgtc
     2581 tccggccgct ggtcgatggc gcggtggccg atcgcgtgca gcaggtgggt cttgccgagg
     2641 ccaacgccgc cgtagatgaa gagcgggttg aacgcctggc ccggcttgtc ggcaacggcc
     2701 atcgccgcgg cgtgtggcag ccggttggac ggcccgacga cgaacgactc gaacgtgtag
     2761 ccggggttga ggccggggcg ctcggccgga gccagcgaca tctgttgcgg ggacgccgga
     2821 tgcggaacgt gggacgtgga gggttcggat cgctgggtgc gggcggcggg tggctccggg
     2881 cgtggcttgg gcttcgggac cctcggggcc ggttcgccag atgcgccacg gacgacgtac
     2941 tcgaccgtga ccgagcggtt gacgagcgca gagatggcgg ccgtgatctc gcggtcgaac
     3001 ttgtggcgca gctgctcgac ggcgaaggcg ttgggtgcgg cgacggtagc cgtctcaccg
     3061 gcgacgccga ccagggaggt cgagcgcaac caggtctcgt agttggcgcg cgagacttgc
     3121 cgctggagat cccccagcgc cgtctgccag agttgctcgt agcggagcgc cgtctgctgg
     3181 ctgctggctg ctgcgtggtc gtcggccata acctgcccgc tcatgctcaa cgtcgtcgat
     3241 ccatccgcat caccaccggg ttcctgacga taccaccctg tggataactt cgtccacaac
     3301 gtgtacgtac agtatagcag gcgggccggt gtgctcgaaa cggctgccgt tcgcgtggta
     3361 ggttttgacg atgcgtgaca atcgatggga gcagaggacg cgtcgatgaa caacgtggat
     3421 ggcggcgacc gcccgatcgg gacgtacgag tacctggatt acagcgaccc ggcggcgatc
     3481 ttccgaccgt acgatccccg cttcccggag gtcgcgcggc gggtagcggc gctgatcgag
     3541 gggcggatgc ccggcgcgcg ggtcgagcac gtcggcagta cggccgttcc gaactgcgcc
     3601 ggcaagggga acatcgacct gctgctacgt tacacggccg gcgagctggc gaccgcccgc
     3661 gaggcgctcg acagcctcgg cttccagcgc cagcagggcg ccaacccgtt cccggagtcc
     3721 cgcccgctac ggctcggcgc gatcgactac gacggcgaga cgttccggct gcacgtccac
     3781 gtcgtcggct gggacgatcc cgaagcggcc gaactgctgc gcttccgcga ccggcttcgc
     3841 gccgacccgg cactggtgga ggcatacgtc gccagcaagc gggccgcgct tacggccggc
     3901 ccgaccgaca acatcgccta caaccgcgcc aagcaaccat tcatccgtgg cgtgatcgct
     3961 gaagaacggc cctgaccccc tttccactca gcacgtttta cgtacgaaca cacagaccgg
     4021 gccttgcggc ccggcctgcg cgggacgggg agggatggag ctgggtacgc tccatgtggg
     4081 tgtcgggaga gtgcgctcgc gcgctgtggg gtctctccgt tactagatat acgctgaacc
     4141 tgtccatttg gttccaccgt ttcgggacca attgtgacaa cgtggcacgc gccgctatca
     4201 gggacacgcc atcattcgcc ctcgaccagc acttgacgct catccgggaa cccaggaaga
     4261 tgctgaggcg attatcgacg acgctcaaat cgcatcaggg ggtggcgatg ccggacacag
     4321 cagatccgaa tacgccagtc acgatcacgc tcgaacgctg gcgttgggag cagattctgc
     4381 tctgcctcga ccgctatgtc gcgtggctgc gcggagagta ctccgacgac gacgctatgt
     4441 ccgatgacgt tgagtgggcg gacgagttgg ggtggatgac ggacgacctt cgagcgatcg
     4501 tgaacgctag tggtagccat ccggtgccgc ctagcacgtc gttcgacgcg ctgtcgccac
     4561 aactgggtga agaagccgcc acattcgtag aaacgccccc gcctaactcg gcaccgaaac
     4621 gtaacccacc gtggaattcg tcggaaaacg atgccgcgat tgccgcatat ttctggatgc
     4681 tcggtgaaca ggacgcattt cgcgacttca acaagcgtgc gaagtaccaa gagttgatgg
     4741 acggacccct gaatcgccga agccgaaagt cgattgagtt caagatgtgt aacatcagcg
     4801 ccgttttcca gcgccatggc atcgagtggg ttgaagggct ggcgccactc ggcaatgctc
     4861 aagctgacct tgccgtggcc gtcgaggaag ccctcgcgga acgaggaatg atcgaccaaa
     4921 cgactgatgc acggtagggc cgcgtcgttg ctgggagcat ct
//
LOCUS       DASUGJ010000540         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1196926, whole genome shotgun sequence.
ACCESSION   DASUGJ010000540 DASUGJ010000000
VERSION     DASUGJ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196926"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="VFV93_12210"
     CDS             <1..1495
                     /locus_tag="VFV93_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5166154.1"
                     /translation="NNDADWTMEFVSEGCRGLTGYDPDELTSGRVTYASIIQPADRDR
                     VWEGVQQALRERSSFSLTYCIATVAGEERWVWEQGQGVFDSHGELQGIEGFIIDVTER
                     ARTFELLEERVADRTRELRMIVEASRIITATLDLNDLFERVFDQAQQVVSFTGASIAV
                     RESEGFFRRASRYTAGGSPLEPQGHRYEMHPDNPIEGRLLRGEALFVHDLLADSAIAA
                     QWRKQAGEKLESAIPHIRSWMSVPMVANDQVVGFMTLTHKEPDRYTTHDVDLVAAIAN
                     HVAIAIENARLHGQARRLAAVEERQRLARELHDSVSQALYGISLGAQTARTLLDRDPA
                     LASQPLDYVVELAQAGMEEMRALIFELRPESLELEGLVVALEKQASATRARQRVQVDV
                     TLCSEPEWLTLSEKEALYRIAQESMHNVIKHAQATWIGLRLDMTERALVLEIVDDGKG
                     FDTGGTFPGHLGLISMRERIATVGGKLAIDSQPGAGTTVRVELPRLV"
     gene            1570..2220
                     /locus_tag="VFV93_12215"
     CDS             1570..2220
                     /locus_tag="VFV93_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166155.1"
                     /translation="MPIRILIADDHSVVRQGLKMFLALDPELEVIGEAENGAEAVRLA
                     GELQPDVVLMDLLMPVMDGITAIENIRATLPDIEVLAVTSVLEDTAVIRAIRAGAIGY
                     MLKDTQADELCRAIKAAAAGQVQLSPEAASRLLREVRAPESPEALTDRETDVLRLLAR
                     GLANKEIAQQLSIGEKTVKTHVSHILSKLGVASRTQAALYAARVGLVPAGEVGERA"
     gene            2217..>2730
                     /locus_tag="VFV93_12220"
     CDS             2217..>2730
                     /locus_tag="VFV93_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5166156.1"
                     /translation="MTGSHEATANAAGRLGLDVLASIMDASLDGIVVIDDNRRYLYVN
                     PAACRILGRPASELIGADILINFPAHQHEQVLGVFKQISLGNGGRNDSTIVLPSGEER
                     QIEFTDTRFMADGRQLRTAIFRDVTESRRQEREARVLAEIAASLTIDQPMEETLDVLT
                     NFVVEETGAKA"
BASE COUNT          503 a          845 c          904 g          478 t
ORIGIN      
        1 caacaacgac gccgactgga caatggagtt tgtctcggag ggttgccgcg ggctgaccgg
       61 ctacgatccg gacgagttga cctcaggccg cgtcacgtat gcctcgatca tccagcccgc
      121 cgatcgcgat cgtgtctggg agggcgtcca gcaggcgttg cgggaacgct cttccttcag
      181 cctgacctac tgcatcgcga ccgtggccgg tgaggagcgc tgggtctggg agcagggcca
      241 gggcgtcttc gacagccacg gagagttgca aggcatcgaa gggttcatca tcgacgtcac
      301 cgaacgcgcc aggacgttcg aactgctgga ggaacgggtt gccgatcgga cgcgggaact
      361 ccgcatgatc gttgaggcgt cacggatcat caccgcgacg cttgacctca acgacctgtt
      421 cgagcgcgta ttcgatcagg cgcagcaggt tgtctcgttt accggggcgt ccattgcggt
      481 gcgtgagagc gaaggcttct tccggcgcgc gtcacgctac accgctggtg gatcgccgct
      541 cgagccgcaa ggccatcgtt acgagatgca tcctgataat ccgattgaag ggcgactcct
      601 tcgcggtgaa gcactgttcg ttcatgactt gctggccgat tccgcgatcg cggctcaatg
      661 gcgcaagcag gccggcgaga agcttgagag tgccatccca catatccggt cgtggatgtc
      721 agtcccgatg gtggcgaacg atcaggtcgt tggcttcatg acgttgacgc acaaggagcc
      781 cgatcgttac acgactcacg atgtcgacct ggtcgccgcg atcgccaacc acgtcgcgat
      841 tgcgatcgag aacgcccgcc tgcacggtca ggcacgacgg ctggcagcgg tcgaagagcg
      901 acagcggctg gcgcgcgaac tgcacgactc ggtctcgcag gcgctctacg ggatctcgct
      961 cggcgcacaa acggccagga cgctgctgga ccgcgatccc gcgctggcat cgcagccgct
     1021 tgactacgtc gtcgaactcg cgcaggccgg catggaggag atgcgagcgt tgatcttcga
     1081 gctccggccg gaatcgcttg agctggaggg cctggtcgtt gcgctcgaga agcaggcgtc
     1141 ggcgacgcgc gcccgccaac gagtacaggt cgacgtaacg ctctgttccg agccggagtg
     1201 gctgacgctc agcgagaagg aagcgttata ccggatcgct caggagtcga tgcacaacgt
     1261 catcaagcac gcccaggcga cgtggatcgg cctccgactc gacatgaccg agagagcgct
     1321 cgtcctggag atcgtggacg atggcaaggg gttcgacacc ggcggcacgt tccccggtca
     1381 tctcgggctg atctcgatgc gcgaacgcat cgccaccgtc ggtgggaagc tggcgattga
     1441 cagtcaaccc ggcgccggaa cgaccgttcg cgtcgaacta ccgcgactgg tgtgacgata
     1501 ttggtatgct gtgagttggc gaacgcctgt cgtgagctgt accgccgtct acctttatag
     1561 agggatagca tgccaatccg catcctgatc gctgacgatc acagtgttgt ccgccagggg
     1621 ttgaagatgt ttctcgccct tgaccccgaa ctggaggtca ttggcgaggc cgaaaatggc
     1681 gcagaggccg tccggctggc cggtgaattg cagccagacg tcgtcctgat ggacctgttg
     1741 atgccagtca tggacggtat cacggcgatt gagaatatcc gcgcgacgct gcccgatatc
     1801 gaggtgctgg ccgtcaccag tgttctggag gacacggctg tcatccgtgc catccgggcc
     1861 ggagcgatcg gctacatgct caaggacacc caggccgacg aactctgccg ggcgatcaag
     1921 gcggctgccg ccggccaggt ccagctctcg ccggaggccg catcgcggct gttgcgtgag
     1981 gttcgcgcgc cggagagccc ggaggcgctg accgatcgcg agaccgacgt gctccggctg
     2041 ctggcgcgcg ggctggccaa caaggagatc gcccagcaac tctcaatcgg cgagaagacg
     2101 gtcaagacgc acgtcagtca tatcctctcg aagctcggtg tcgcctcgcg cacccaggcc
     2161 gccctctacg cggcccgggt cggactcgtt ccggccgggg aggttggcga acgggcgtga
     2221 ctggctcgca cgaggctacc gctaacgccg ccgggcggct cgggctggat gtcctggcga
     2281 gcatcatgga cgcctcgctc gacggcatcg tcgtcatcga cgacaaccgg cgctacctct
     2341 acgtgaatcc ggccgcctgc cggattcttg gccgtcccgc gagtgagctg atcggcgcag
     2401 atatcctgat caacttcccg gcccatcagc acgagcaggt gctgggtgtc ttcaagcaga
     2461 tctcactcgg caatggcggg cggaacgaca gcacgatcgt gttgccgtcc ggcgaggagc
     2521 gccagattga gttcaccgac acgcgattca tggccgacgg ccggcaatta cggacggcca
     2581 tcttccgcga cgttaccgaa tcccgccgcc aggagcgcga ggcacgcgtg ctggctgaga
     2641 tcgccgccag cctgacgatc gaccagccga tggaagagac gctcgacgtt ctgacgaact
     2701 tcgtcgttga agagaccggt gcgaaggcgg
//
LOCUS       DASUGJ010000541         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1474163, whole genome shotgun sequence.
ACCESSION   DASUGJ010000541 DASUGJ010000000
VERSION     DASUGJ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474163"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VFV93_12225"
     CDS             complement(<1..539)
                     /locus_tag="VFV93_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166157.1"
                     /translation="MDDKVATHQEVMQGQARTAERSQRSDQLLLFGSGALAGLVTVAF
                     MLLLRRVTETDSLLEIVGEALVQLMPMAMFAFLLETFREAAKPLLVVGIVLGMMLAGG
                     GIARMDGGPARAASPGRRFWRGLLVALSIWVPLAIFAVIVVSIGTVAPITNRSVAALA
                     LILLADAIVFVLALYLFFPL"
     gene            807..2489
                     /locus_tag="VFV93_12230"
     CDS             807..2489
                     /locus_tag="VFV93_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX5166158.1"
                     /translation="MSAVPELPVQPIYPFASKVPTIRLLHDESSQHPFATTIAAAWSC
                     YGARPARVENVLGLLDESLDDGDDLRPGRRNRALRLYRDLFDAGHHTTFQHANFVFVL
                     DGVSRLAIWSFFHHHPHYNSEQVSQRYREVSGKVMVTPDLTERQSAIYKAAVERAIDG
                     YRRLGDLLSPGLAERYGQVFPARRKADGAEAARRVADAVQKKAQEVARYVLPLATPAH
                     LYHTINGLTLLRYYVLVNQLDVPSEVRYIVNAMVGEVLKLDPNFLGAPGYPLDLQVLG
                     ASDTLEFGALAELQGTLAASHDETFFREYDEALGGLDSKLVGYNPNAEAVLADAVRTV
                     LGASRAAMSDADAMATVLDPAKNEYLGHSLFLAMNSKLMQTLNHVPFTFQKRISGAED
                     AQNQRHRGTLSSSPVLSAHMRVAPDVIVPDEVRRSDAALDEYHATVQALWDAKNQLLD
                     DGVAPEKVLYLLPNSHNVRFHESGTLLTYYWKWVKRLCYNAQREIFDTARQETEQVSA
                     IFPTIGRHVSAPPCVIRSRAGNKPFCPEGERFCGVPVWRDYDPKTLALRRVL"
     gene            2639..3082
                     /locus_tag="VFV93_12235"
     CDS             2639..3082
                     /locus_tag="VFV93_12235"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX5166159.1"
                     /translation="MDFKGDVTINAPREKVWAFLTDPHQVTQCAPGLQSIEVVDDTHF
                     KCVVRAGVGMIKGNFNFDIEWVEMNEPNNARMSANGKIPGSAVAMNSSLALADNGSGG
                     TDMHWESNVKVSGTIAGVGARLMSGVADRMTKDIFACIQSKLDTP"
BASE COUNT          636 a         1111 c         1048 g          587 t
ORIGIN      
        1 aggggaaaga acagatagag cgccagcacg aagacgatcg cgtcggccag caggatcagc
       61 gcaagggcgg cgacgctgcg gttcgtgatt ggcgcgaccg tcccgatcga gacaacaatc
      121 acggcgaaga tcgccagcgg cacccagatc gagagcgcca ccagcagccc acgccagaag
      181 cgcctgccag gtgaggccgc ccgtgccgga ccgccgtcca tccgcgcaat gccgccgccg
      241 gccagcatca tccccagcac gatcccgaca accagcagcg gcttggccgc ctcgcgaaac
      301 gtttccagca ggaaggcaaa catcgccatc ggcatcagct gcaccaacgc ctcgccgacg
      361 atctccagca gcgagtcagt ttcggtcacc cgccgcagca gcagcatgaa ggcaaccgtc
      421 accaggccag ccagtgcgcc tgagccgaac agcagcagtt gatcgctgcg ctggcttcgc
      481 tctgccgttc gcgcctgccc ttgcatcact tcctgatggg tcgccacctt gtcgtccatc
      541 ccactgaccg ccggaaccgt cgtccgtctg ccttcaacct accaccggac gcttacgcag
      601 atatgacgct actgtccgga acgcctcgcc cctaccggcg gaagatccag cagccttctt
      661 cgtggatacg taatacaata gcgtgacgaa catctgttcg ttgccgtcgc ctgcctgtga
      721 ccgtccggca catcttgtca cctgtcgcgc cagcctgtac cctgtgtacg tacactatct
      781 cgtgtctccg cgatccgggg gaaaccatgt ccgcagtgcc tgagcttcca gtccaaccga
      841 tctatccatt cgcatcaaag gtaccgacga tccggctgct gcacgatgaa tcgtcgcagc
      901 atccgttcgc gacaacgatc gcagcggcct ggtcatgcta tggcgcgcgg ccagcgcggg
      961 tcgagaatgt gctcggcctg ctggacgagt cgctcgacga tggcgatgac ctccggccgg
     1021 gacgccgcaa ccgggcgctg cgactctacc gcgatctgtt cgatgccggc catcacacga
     1081 cctttcagca tgccaatttc gtcttcgtcc tggatggcgt ctcgcgcttg gcgatctggt
     1141 cgtttttcca ccaccacccg cattacaact cggagcaggt cagccagcgc taccgcgagg
     1201 tctccggcaa ggtgatggtg acgcccgatc tgaccgagcg acagagcgcg atctacaagg
     1261 cggccgtcga gcgggcgatc gatggctacc ggcggctggg cgatctgctc tcgccggggc
     1321 tggccgagcg ctacgggcaa gtcttcccgg cccgccgcaa ggcggacggc gccgaagcgg
     1381 cccgacgggt tgccgacgcc gtccagaaga aggcgcagga agtcgcacgg tatgtcttgc
     1441 cgttggcaac gccggcccac ctctaccaca cgatcaacgg cctgacgctg cttcgctact
     1501 acgtgctggt caaccagctc gatgtgccgt ctgaggtacg ctacatcgtc aacgcgatgg
     1561 tcggcgaggt gctgaagctt gacccgaact tcctcggtgc gcccggttac ccgctcgatc
     1621 tccaggtgct cggggcgtcc gacacactgg agtttggagc gctggccgag ctacagggaa
     1681 cgctggcggc cagccacgac gagacattct ttcgtgagta cgacgaggca ctcggtggac
     1741 tggattcgaa gctggtcggc tataacccga acgccgaagc ggtgctggcc gacgcagtcc
     1801 ggacggtgct gggcgccagc cgcgccgcga tgagcgatgc cgacgcgatg gcaaccgtcc
     1861 tcgatccggc gaagaacgag tatctcggcc actcgctgtt cctggcgatg aacagcaagc
     1921 tgatgcagac gctgaaccac gtgccgttca cgttccagaa gcgcatcagt ggcgctgaag
     1981 acgcccagaa tcagcgccac cgtgggacgc tctcgtcgtc cccggtgctg tcggctcaca
     2041 tgcgcgtcgc gccggacgtg atcgtcccgg acgaggtgcg ccgctccgac gcggcactgg
     2101 atgagtacca cgcgaccgtt caggcgctct gggatgcgaa gaatcagttg ctggacgatg
     2161 gtgtagcgcc cgagaaggtg ctctacctgc tgccgaacag ccacaacgtg cgtttccatg
     2221 agagcgggac gctgctgacc tactactgga agtgggtcaa gcggctctgc tacaacgccc
     2281 agcgggagat cttcgacacc gcccgccagg aaaccgagca ggtctcggcc atcttcccga
     2341 ccattggccg gcacgtcagc gccccaccct gcgtgatccg ctcgcgagcc ggcaacaagc
     2401 ccttctgccc cgaaggcgag cgcttttgtg gcgtccccgt ctggcgcgac tacgacccga
     2461 agacgctggc gctgcggagg gtgctgtagc ggaagattcg ggaaccagga cacgggattc
     2521 ggggtttcga agaggcaagt ccctccgaga cccccgaatc ccgtgtcccg gttcccgttc
     2581 ccttatactc cagtggcaac agcggtacgc cgcacgcagc cgccgggaag ggagctccat
     2641 ggatttcaag ggtgatgtga cgatcaacgc cccgcgtgag aaggtctggg cctttctcac
     2701 cgatccgcat caggtgacgc agtgcgcgcc agggttgcag agcatcgagg tggtcgacga
     2761 cacgcacttc aagtgcgtcg tccgggccgg tgtcggcatg atcaagggca acttcaactt
     2821 cgacatcgag tgggtcgaga tgaacgagcc gaacaacgcg cggatgtcgg ccaacggcaa
     2881 gatccccggc tcggccgtcg cgatgaacag ctcgctggcg ctggccgata acggcagcgg
     2941 cgggaccgac atgcactggg agtcgaacgt caaggtcagc ggcacgattg ctggcgtcgg
     3001 cgcgcggctg atgagcggtg tcgccgaccg gatgacgaag gacatcttcg cctgcatcca
     3061 gtcgaagctc gacacgccgt agccttcatc gttggcgaac gcgcccaacc cgcatcgctg
     3121 catttgcggc ttgcaggttg ggcgcttgtc gttgcccaat ccaatgtccc aatccggtac
     3181 gggcgatatc gcgcgtaccg gattgggaca ttgggaattg acagcgtgcg cgagcgtcgt
     3241 tgatgcacag tccacccggg tggactgtag cgcatcatga tcgttatgtc acgtcctcat
     3301 cgcggcttgt cggttctgat cgccgccagt atggtttgac acatcgatca ttggtgcagc
     3361 caacctggac gaaggtggtg gc
//
LOCUS       DASUGJ010000542         4566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1891148, whole genome shotgun sequence.
ACCESSION   DASUGJ010000542 DASUGJ010000000
VERSION     DASUGJ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891148"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..3724)
                     /locus_tag="VFV93_12240"
     CDS             complement(149..3724)
                     /locus_tag="VFV93_12240"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5166160.1"
                     /translation="MTDPFARYGLFIYRTRWIVLIAWLVILGVCGAVLAPKAGSVLKA
                     GGIEDPNSEAATATRILEEDFNNSALNNAVIVFRSDSLTVDDPAYRDAVVGNAERVAG
                     IEGVNSVVTFYTIGDPSLVSADHKTTVAIVSLAGDEGEAQEIVPDVRDAIQEADGIEH
                     FVTGTPAVNHDFQVTSEEDLRRSEMVTIPLVLILLALTFRTIISAAVPLALGAAAVVT
                     ALSFIYLIGERTDLSIFALNVASMIGLGLGIDFSLIVVNRYREEYALRKDAGLATAIT
                     MATAGRSIAYSGITVMLGMTVLTLMIDLMVVRSISLGVLLVAFTALLGGLTLLPALLG
                     ILNYRLEWLRILPQRREKSETSGFWYDFSHAIMRRPWAWLFVSIVILATIAWPARNLD
                     LLGSSAGLLPSETESVQGVNILDQEFAKNLLTPIQIVMKSDQPDGVFTPEFLKALDEF
                     TNVLDADPRSSQVQSLATFMAAEPRFDGRFYRITRNDFTPFPPMNPDDPTQLTNGISV
                     QPFIDVYVDAVPITPAYFGFATFTFAAGESHQLQVAPALQVYRVNSGTLQVQATGSVT
                     YWSAANFGNPDAGQVVSDGSTLQLSPGDQLVIPEQTPVTITAVDQVELLTAISFNVRP
                     GTGAQDTWTEGQPTSDPFAGIERQVIGGGIGSSFPTGRTNIKLELAHTDPGAHFPRHL
                     HPGPELIVVEEGTLTIHSSPEMVMTGADGRVREGPFDVPIDLGVGGKAVVQGPGIHRA
                     ENNTTGETSIWSLRFLDVNQPAFTLVGTTELANQFVNLNGDASAAVINVTSPYGPYDD
                     EHEDFVHEIRTLIAPSIDGMENYDVFVGGQSASFTDFSDTLYNKFPLLVGAVLLLTYI
                     ILLMFFQSVFLPMKAILMNLASVLATYGALVMIFQYGWGDSLFGFDNLGAVGVVTPAI
                     LFVILFSLSTDYEVFMLSRVKEYYHETHNNEEAVAAGLQHTAGVITAAGVILIGVFGS
                     FATAGIITIKEIGLGLAIGVLIDTTVVRIILVPATMRLMGDTNWYMPAWLKKIVPELR
                     EGPVPALAPAGGGAMVASVRGNGHAASPNGDPFVPAAMPERMAGQLVRTGGPMNADVI
                     TLPRARPFRIGRDEKSDLQVFDVRISRFHAKIEHRRGEYIITDLNSTNGVYVNGERIT
                     EPTVLRQNDALEIANTGAVTFRFEQRSLSEPPNVMAAGTPSTTDD"
     gene            complement(3806..4069)
                     /locus_tag="VFV93_12245"
     CDS             complement(3806..4069)
                     /locus_tag="VFV93_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166161.1"
                     /translation="MEEQPGRTSQPAAGNLVAQVNMVPEDEPVDTGQWPATAGDGPVT
                     AHGTIPGARSNPLQAILLGVLALVVILASMFVILQGVFEMIPS"
     gene            complement(4044..>4566)
                     /locus_tag="VFV93_12250"
     CDS             complement(4044..>4566)
                     /locus_tag="VFV93_12250"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5166162.1"
                     /translation="EDAAFFLYVATFHQPEMAPPGMHSLKLEAYTTLGAKGIDWERDK
                     EDIAKVFVRRAEKLIPNLGQHIVTQALRTPQDLYNDTGNSEGAFAGWAFTPELLSRER
                     PSQRSPVPGLYLAGHWTTPTAGVPWVMVSGFNTAGLVSADLGRQRLRSHAIQPAKRGR
                     MEAGDDGRTTRSH"
BASE COUNT          813 a         1406 c         1528 g          819 t
ORIGIN      
        1 gatcggcagc gtcccggaga cgatcatcgt gctggacgtg ttgttgacga tctcctcgat
       61 ctcgtccgga tcgaagtact gccgccagta ttcgcttgcc agtccaccat cgcgctgtgc
      121 catgccctgg atcctcggct cgtttctgct agtcgtcggt cgtactcggc gtgccggccg
      181 ccatcacgtt cggcggttcg gagagcgagc gttgctcgaa gcggaatgtg acggcgccgg
      241 tgttggcgat ctccaacgcg tcattctgcc gcaacacggt cggttcggta atccgctcgc
      301 cgttcacgta gacaccgttc gtgctgttca ggtccgtgat gatgtattcg ccacggcgat
      361 gctcgatttt ggcatggaat cgcgagattc gcacgtcgaa gacctgcaga tccgatttct
      421 cgtcgcgccc gatccggaac ggccgcgcgc gtggcagggt gatgacatcc gcgttcatcg
      481 gcccgccggt cctgacgagt tggcccgcca ttcgttccgg catcgcggcc gggacgaacg
      541 gatcgccatt cggcgaggct gcgtggccgt tgccacgaac ggacgccacc attgcgccgc
      601 caccagccgg cgccagcgcc ggaaccggac cctcgcgcag ttccgggacg atcttcttga
      661 gccaggccgg catgtaccag ttggtgtcgc ccatcagccg catcgtggcc gggacgagga
      721 tgatacggac gacggtcgtg tcgatcagga cgccgatcgc cagcccgagc ccaatctcct
      781 tgatcgtgat gatcccggcc gtcgcgaacg agccgaagac gccgatcagg atgacaccgg
      841 cagccgtaat gacgccggcc gtgtgctgga gtccggccgc gacggcttcc tcgttgttgt
      901 gcgtctcgtg gtagtactcc ttgacccgcg agagcatgaa gacctcgtag tcggtcgaga
      961 gactgaagag gatcacgaac aggatcgcgg gcgtgacgac gccgaccgcg ccgaggttgt
     1021 cgaatccgaa cagcgaatct ccccagccat actggaagat catcaccagt gcgccgtagg
     1081 tcgccaggac cgaggcgagg ttcatcagga tcgccttcat cggcaggaag accgactgga
     1141 agaacatcaa caggatgatg tacgtcagca gcaggacggc gccgaccagc agcgggaact
     1201 tgttgtatag cgtgtcgctg aagtcggtga agctggccga ctgaccgccg acgaagacat
     1261 cgtagttctc catcccgtcg atgctgggcg cgatcagcgt ccggatctcg tgaacgaagt
     1321 cctcgtgctc atcgtcgtat ggcccgtacg gcgacgtgac gttgatgacg gcggcgctgg
     1381 cgtctccgtt gaggttgacg aactgattgg ccagctcggt cgtgccgacc agcgtgaagg
     1441 caggctggtt gacgtcgagg aagcgcagcg accagatact cgtctcgccg gtcgtgttgt
     1501 tctcggcgcg gtggatgcct ggcccctgca cgacggcctt gccgcccacg ccaaggtcga
     1561 ttggcacgtc gaacggtcct tcgcggaccc ggccatcagc gccagtcatc accatctccg
     1621 gggaagagtg gatcgtcagc gttccttcct cgacaacgat cagctccggg ccggggtgga
     1681 ggtggcgcgg gaagtgtgcg ccgggatcgg tgtgtgccaa ttccagcttg atattcgtgc
     1741 ggccggtcgg gaaagagctg ccgatgccgc caccgatgac ctgccgctcg atgccggcga
     1801 aggggtcgga ggtgggctgg ccctcggtcc acgtatcctg cgctccggtg ccgggacgaa
     1861 cgttgaacga gatggcggtc agcagttcga cctgatcgac agcagtgatc gtgaccggcg
     1921 tctgttcggg aatgacgagc tggtcgccgg gtgagagttg cagcgtcgag ccgtcggaga
     1981 cgacctgacc ggcgtccgga ttgccgaagt tggcggctga ccagtaggtc accgaaccgg
     2041 tcgcctggac ctggagcgtc cctgagttga cgcgatagac ctggagagcc ggcgccacct
     2101 ggagttgatg actttcacct gccgcgaagg taaacgtggc gaagccgaag taggccggtg
     2161 tgatcgggac ggcatcgacg tagacatcga tgaacggctg gaccgagatg ccgttcgtca
     2221 gctgggtcgg gtcatccggg ttcatcggtg ggaacggcgt gaagtcgttg cgggtgatgc
     2281 ggtagaagcg gccatcaaag cgcggctcgg cggccatgaa ggttgccagt gactgcacct
     2341 gcgagctgcg cggatcggcg tccagcacgt tcgtgaactc gtcgagcgct ttcagaaact
     2401 ccggcgtgaa gacgccgtcc ggctggtcgg acttcatcac gatctggatc ggtgtcagca
     2461 ggttcttggc gaactcctgg tcgagaatgt tgacgccctg gaccgactcc gtctcgctcg
     2521 gcagcagccc ggccgatgag ccgagcagat cgaggttgcg ggccggccag gcgatggtcg
     2581 ccaggatgac aatcgagacg aacagccagg cccacggccg gcgcatgatc gcgtggctga
     2641 agtcgtacca gaagccgctc gtctcgctct tctcgcggcg ttgcggcagg atccgcagcc
     2701 attcgaggcg gtagttgagg atgccgagca gcgccggcag cagcgtcagg ccaccgagca
     2761 acgccgtgaa ggccaccagc aggacaccga ggctgatcga gcgaacgacc atcaggtcga
     2821 tcatcaacgt cagaacggtc atccccagca tgacggtgat gccggaatag gcgatcgagc
     2881 gtccggccgt cgccatcgtg atcgccgtcg ctaacccggc gtctttgcgg agggcatact
     2941 cctcccggta acggttgacg acgatcaacg agaagtcgat cccgaggccg agaccgatca
     3001 tcgaggcgac attcagggcg aagatggaga gatcggtccg ctcgccgatc agatagatga
     3061 acgacagcgc cgtcacgacg gccgccgcgc cgagcgccag cgggaccgcc gccgagatga
     3121 tcgtccggaa cgtcagcgcc agcaggatca gcaccagcgg gatcgtcacc atctcggagc
     3181 gtcgcaggtc ttcctcgctg gtgacctgga agtcgtggtt gacggccggc gtgccggtca
     3241 cgaaatgctc gatgccgtcg gcttcctgga tggcgtcccg gacgtccgga acgatctcct
     3301 gcgcctcgcc ctcgtcgccg gccagcgaga cgatcgcgac cgtcgtcttg tggtcggccg
     3361 agacgagcga tgggtcgccg attgtgtaga aggtaacgac gctgtttacg ccttcgatac
     3421 cggccacgcg ctcggcgttg ccgacgacgg catcgcggta ggcggggtca tcgacggtga
     3481 ggctatcgga ccggaagacg atgacggcgt tgttgagcgc cgagttgttg aagtcctctt
     3541 cgagaatgcg ggtcgcggtc gcagcttcgg agttgggatc ttcgatgccg cccgccttga
     3601 ggaccgatcc ggccttcggc gcgaggactg caccgcagac gccgaggatc accaaccagg
     3661 cgatcaggac gatccagcgc gttcgataga tgaacaggcc gtaacgggca aacggatcag
     3721 tcatcgttcc aagctcctcg ccccaccggg agaaccagca ttgtatcgcc tcattggtgt
     3781 cgttcagatc gtaccgcgcg ggtgcctaac tcgggatcat ctcaaagacg ccttgcagga
     3841 tgacgaacat cgacgcgagg atgacgacca gcgccagcac cccgagcaga atcgcctgga
     3901 gcggattgga ccgggcgccc ggaatggtgc catgtgcggt gactggtcca tcgccggccg
     3961 tcgcgggcca ttgtccggtg tcaaccggct cgtcctcggg gaccatgttg acctgtgcga
     4021 cgaggttccc cgcagcgggc tgactagtgc gacctggttg ttcttccatc gtcgccggcc
     4081 tccatccgac ctcgctttgc tggctggatc gcgtgtgagc gtaagcgctg gcgtcccaga
     4141 tcggcgctca cgagtccggc cgtgttgaag ccggagacca tcacccaggg cacaccggcc
     4201 gtcggcgtcg tccagtgacc ggccaggtag agccccggga cgggcgagcg ttgcgacggt
     4261 cgctcacgag acagcaactc cggcgtgaag gcccagccag cgaacgcgcc ctccgagttg
     4321 ccggtgtcgt tgtagagatc ctgcggcgtc cgcagcgcct gggtcacgat gtgctggccc
     4381 agattcggaa tcagcttctc ggcgcgccgg acgaacacct tggcgatgtc ttccttgtca
     4441 cgctcccagt cgatcccctt cgccccgagc gtcgtgtacg cctccagctt gagcgagtgc
     4501 atgccgggcg gggccatctc cggctggtgg aaggtcgcga catacaggaa gaaggcagcg
     4561 tcctcg
//
LOCUS       DASUGJ010000543         5503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1474502, whole genome shotgun sequence.
ACCESSION   DASUGJ010000543 DASUGJ010000000
VERSION     DASUGJ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474502"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFV93_12255"
     CDS             complement(<1..755)
                     /locus_tag="VFV93_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX5166163.1"
                     /translation="MSVAEIPAAPTLADVLLARRVVNRYLAPTPVLRPEGLARELGFE
                     VILKCENLQPVGAFKVRGGMYLMSRLDDDERTRGVVTASTGNHAQSIAYAAREFGVRA
                     VIFMPENPNPVKLASTKALGAQVIEFGRDFDECRIEAGEYAAREGMRFIHSANEPDLI
                     AGVGTYALELIEEAPDLDVVIVPVGGGSGVCGTALVFKSMRPETLVIAVQSEGMPVVY
                     RSFHERQLLSLEGGSTWAEGLATRVAFELPYAM"
     gene            988..1824
                     /gene="pyrF"
                     /locus_tag="VFV93_12260"
     CDS             988..1824
                     /gene="pyrF"
                     /locus_tag="VFV93_12260"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5166164.1"
                     /translation="MTTGTTVIPFADKLRTAQAANRSLLCVGLDPDPSRFPAAVGNTP
                     ESIIAFNRAIIEATSDLACCYKPNLGFYLAYGALGVEALAQVRRDVPAHIPVLLDAKF
                     GDIGITSVGYARAAFETWNVDALTVNPFLGRDALDPFLSYSDRGIFVLAKTSNPGSGL
                     LQDRQLAGGGSVTMAVVNDALTWNAAGNVGLVVGATYPEQLAEVRASASDLPILVPGV
                     GAQQGDLAAAVQVGLDANRAGLLINASRAISYASSGPDFKDAARAAAEALRAQIESAR
                     IS"
     gene            1951..2721
                     /locus_tag="VFV93_12265"
     CDS             1951..2721
                     /locus_tag="VFV93_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166165.1"
                     /translation="MSAPDDRSGQFGHDGEEDLFDDIREFLTEQSSDRIFEDLAQAYG
                     LARNFVDRELRGIDEFDRMLRALERLVDEVFEGEFSGEQRAKIAEGFYLYGLAHYSLM
                     QQIISHGHGQGEEEDDDEPVKPLVIYDLWFPGDVAIERADDGLYMHISDGRIDVSIYL
                     GATPNERSALVDLVDHWGYAASNTGPIERDAEPPGTIPMLTIELDYSGSELVDAHDHV
                     DVLFFDDEGEPIVRMHARASDLRAIVDEMQAAERDDSL"
     gene            2829..4760
                     /locus_tag="VFV93_12270"
     CDS             2829..4760
                     /locus_tag="VFV93_12270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5166166.1"
                     /translation="MEPRRYTLEMAVDLRVPSNLRLSPDNSQIAFCVAPIGHREKDPT
                     ATIYVAASDDSTLPRPITGSEHNNTIPRWSPDSGSIAFLSDRPKRGELQLHVVPAGGG
                     EPLRLTNLQGGVDQPDWLPDGRSIAFTARRRALAGQKEPESDIRVASERWKPKAIATI
                     APTGGAPVIVGPGEGHVWTFSYSPDGARIAAIVSETESLAVAFSTSHLIVFDADGGNE
                     RDLVELAGVPSQISWSPDGRQVAVVGSQHGNRDYVNVIVVDVDSGEVTALDERGSTPN
                     WASFAGDDLLVHSVHIQNTRVERTDPRGSEWEQVDLGPDATSGWIDARVSIHATDDAI
                     VFGVAQPYRPADIYVARLGQPAVRITDLNPHLAGLPMAEMEHITWTATDGTETHGWLL
                     LPPDAAPNTRLPLITEVHGGPSAQWGNWFYGTWHDWAQILANEGYAVFLPNPRGSTGR
                     GGAFTAANKYDFGGGDFDDIMTGIDMLIERGVADPDRLGICGWSFGGFMTAWAVTHTD
                     RFKAAFAGASPTNWVSKIGTTDIRPANEWNLGTVNDEPDRPWQQSPIRYVKHAKTPTL
                     LVQGGADVRVPPTQSWEFYNALNALGVPTDLVVYPRQGHSFHERAHMLDLLQRLTGWF
                     ARYLPIEGANQPRQRDGEA"
     gene            4757..5215
                     /locus_tag="VFV93_12275"
     CDS             4757..5215
                     /locus_tag="VFV93_12275"
                     /inference="COORDINATES: protein motif:HMM:NF038048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="HEX5166167.1"
                     /translation="MKLAEALILRADNQRRIEQLKQRIVQNAITQEGSDPAEDPNALV
                     QEMERTADDLATLIQQINATNSSATLDDGTTLSAALARRDVLKLKHAIYRDAAQAASQ
                     TGNRYMRSELRMTPQIDVATIQQRADDLAREHRELDTRIQETNWRTDLIE"
BASE COUNT         1017 a         2077 c         1534 g          875 t
ORIGIN      
        1 atcatcgcat acggcaactc gaacgcgacc cgcgtcgcca gcccttccgc ccaggtgctg
       61 ccaccttcca gcgacagcag ctgccgctcg tggaacgagc ggtacaccac cggcattccc
      121 tcggactgaa cagcgatcac cagcgtctcc ggccgcatcg acttgaacac cagcgccgtc
      181 ccgcagacgc ccgagccgcc accaaccggc acgatcacga cgtccagatc cggcgcttcc
      241 tcgatcagct ccagtgcgta ggtcccaaca ccggcgatca gatccggctc gttggccgag
      301 tggatgaacc gcatcccctc gcgcgccgcg tactcaccgg cctcgatccg gcactcgtcg
      361 aagtcgcgac cgaactcgat cacctgcgcg cccagcgcct tcgtcgacgc cagcttgacc
      421 ggattcgggt tctccggcat gaagatcacc gcccgcaccc cgaactcccg cgccgcgtag
      481 gcgatcgact gcgcgtggtt cccggtcgag gccgtcacaa ctccccgcgt gcgctcgtca
      541 tcgtcgagcc ggctcatcag gtacatacct ccacgtacct taaacgctcc aaccggctgc
      601 aggttctcac atttgaggat cacttcgaag ccaagctcgc gggccagtcc ctctggccgc
      661 agcaccggtg tcggcgccag atacctgttg acgacccttc gcgccagcag cacgtccgcc
      721 agtgtcggtg ctgccggaat ttcggcgacg ctcaaaccag tcgatccttc agaaacagaa
      781 cgacgcaaag ctcttctccc atttgatcgc cgatgatgct ccaactcgat ccgtcacctc
      841 aactggcgaa cggcaccgag ccattgacga tcgtgaggcc gggttgttat cgaaccggcc
      901 ggagtatatc atcggacgcg gcgaccaacg cgggttgcgg tcatcaaatg cgcaaaggca
      961 tgcttgcgcg aaaggggcga attcgtaatg acaaccggca cgacggtgat ccccttcgcg
     1021 gacaagctgc gcaccgccca ggccgccaac cgctcgctgc tctgcgtcgg cctcgacccc
     1081 gatccgtcgc gcttcccggc cgccgtcggc aatacgccgg agtccatcat cgccttcaac
     1141 cgcgccatca tcgaggccac atccgacctg gcctgctgct acaagcccaa cctcggcttc
     1201 tacctcgcct atggcgcgct cggcgtcgaa gccctcgccc aggtccgccg cgacgttccg
     1261 gcccacatcc ccgtcctgct ggacgccaag ttcggcgaca tcggcatcac cagcgttggc
     1321 tacgcccgcg ccgccttcga gacctggaac gtcgacgccc tgaccgtcaa cccgttcctc
     1381 ggccgtgacg cacttgatcc tttcctttcc tacagcgacc gcggcatctt cgtcctcgcc
     1441 aagacctcca accccggcag cggcctcctc caggatcgcc agctggccgg tggtgggagt
     1501 gtcacaatgg ccgtcgtcaa cgacgccctc acctggaacg ccgctggcaa cgtcggcctc
     1561 gtcgtcggcg caacgtaccc tgaacaactc gccgaggttc gtgcttccgc gtcagatctc
     1621 ccgatcctcg ttcccggcgt tggcgcccag cagggcgacc tcgcagccgc cgtccaggtc
     1681 ggcctcgacg ccaatcgcgc cggcttactt atcaacgcca gccgcgccat ctcgtacgcc
     1741 tcatccggcc ctgacttcaa ggacgccgcc cgcgccgccg ccgaagcgct ccgtgcccag
     1801 atcgaatccg cccgcatctc ctagatcgcc ggaccagcgc cagcgttcag cgtcacgcac
     1861 atcttccacg ctgccaactg ctctggtatg gtacgccgcg agtggacgcg ccccgattgg
     1921 ccccggccgg cgggggtagg gagtacggca atgtccgcac ccgacgacag gtccggccag
     1981 ttcggccacg atggcgaaga ggacctcttc gacgacatcc gcgagttcct gaccgagcag
     2041 tcctccgacc gcatcttcga agacctggcc caggcctacg gcctcgcccg taacttcgtc
     2101 gatcgcgagt tgcgcggtat cgacgagttc gaccggatgc tgcgcgccct cgaacgcctc
     2161 gtcgacgagg ttttcgaagg cgagttctcc ggtgagcagc gcgccaagat cgctgaaggg
     2221 ttctacctct atggcctggc ccactactcg ctgatgcagc agatcatcag tcatggccac
     2281 ggacagggcg aggaggagga cgacgacgaa ccggtcaagc cgctcgtcat ctacgacctc
     2341 tggtttcccg gtgatgtcgc catcgagcgg gctgacgacg gcctctacat gcacatcagc
     2401 gacggccgga tcgacgtctc gatctacctc ggagcgacgc ccaacgagcg cagcgccctg
     2461 gtcgacctcg tcgaccattg gggctacgcg gcatccaaca ccggcccgat cgagcgcgat
     2521 gccgaaccac ccggcacgat tccgatgctg acgatcgagc tcgactactc cggctccgaa
     2581 ctggtggacg cccatgatca cgttgacgtc ctcttcttcg acgacgaggg cgaaccgatc
     2641 gtccgcatgc acgcccgtgc cagcgacctg cgcgccatcg tcgacgagat gcaggccgcc
     2701 gaacgcgacg attcgctcta gtgtgtgcaa cacgagtcag ttcgaccggc cccgatccct
     2761 tatccctgtc atccctactg gcgtggggat gacggggatc gtgacccgag atagcgaaag
     2821 gaaactccgt ggaaccacgc cgctacactc ttgaaatggc cgtcgacctg cgcgttccct
     2881 ccaacctgcg cctctctccc gacaacagcc agatcgcctt ctgcgtcgcc ccgattggcc
     2941 accgcgaaaa ggacccgacc gcgaccattt acgtcgccgc cagcgatgac tcgaccctgc
     3001 cccgcccgat caccggctcc gagcacaaca acaccatccc gcgctggtcg cccgattccg
     3061 gctcgatcgc cttcctgtcc gaccgcccca agcgcggcga actccagttg cacgtcgttc
     3121 cggccggagg cggcgaaccg ctgcgcctga ccaacctcca gggcggtgtc gaccagcccg
     3181 attggctccc ggacggtcgc agcattgcct tcaccgctcg ccgccgcgct cttgccggcc
     3241 agaaggagcc ggaatccgac atccgcgtcg ccagcgagcg ctggaagccg aaggcgatcg
     3301 cgacgatcgc cccaaccggc ggcgcgcctg tcatcgtcgg ccctggcgag ggccacgtct
     3361 ggaccttcag ctactccccc gacggcgccc gcatcgccgc catcgtatcc gagaccgaga
     3421 gcctggccgt cgccttctcc acctcccatc tgatcgtctt cgacgccgac gggggcaatg
     3481 aacgcgatct cgtcgagctg gcaggcgttc ccagccagat cagctggtca ccggatggcc
     3541 gtcaggtcgc cgtcgtcggg tcgcagcacg ggaatcgcga ttatgtcaat gtcatcgttg
     3601 ttgatgtgga ctcgggcgag gtaactgctc tcgacgaacg cggctcgact cccaactggg
     3661 cgtccttcgc cggcgacgac ttgctggtcc acagcgtcca catccagaac acgcgcgtcg
     3721 agcgcaccga cccgcgcggc agcgaatggg aacaggtcga cctcggaccc gacgccacca
     3781 gcggctggat cgacgcccgc gtctcgatcc acgctaccga cgacgcgatc gtcttcggcg
     3841 tcgcgcagcc ataccgcccg gccgacatct acgtcgccag gctcggccag cctgctgtcc
     3901 gcatcaccga tctcaacccg catctggccg gactaccgat ggccgagatg gagcacatca
     3961 cctggaccgc caccgacggc accgagaccc acggctggct cctgctgccg cccgacgccg
     4021 cgcccaacac ccgcctaccg ctcatcaccg aagtccacgg cggcccatcc gcccagtggg
     4081 gcaactggtt ctacggcacc tggcatgact gggcccagat tctcgccaac gaggggtacg
     4141 ccgtcttcct gccaaacccg cgtggcagca ccggtcgtgg cggtgcattc acggccgcca
     4201 acaagtacga cttcggtggc ggcgacttcg acgacatcat gaccggcatc gacatgctga
     4261 tcgagcgcgg tgtcgccgat cccgaccggc tcggcatctg cggctggtcc ttcggcggct
     4321 tcatgacggc ctgggccgtc acccacaccg accgcttcaa ggccgccttc gccggcgctt
     4381 ccccgaccaa ctgggtctcc aagatcggca cgaccgacat ccgccccgcc aacgagtgga
     4441 acctcggcac cgtcaacgac gagccagacc gcccctggca gcagtctccc atccgttacg
     4501 tcaagcacgc caagacgccg accctgctcg tccagggcgg cgccgacgtc cgcgttcccc
     4561 cgacgcaatc ctgggagttc tacaacgccc tcaatgccct cggcgtcccg accgacctcg
     4621 tcgtctaccc ccgccagggc cattcgttcc acgaacgcgc ccacatgctc gacctcctcc
     4681 aacgcctcac cggctggttc gccaggtacc tcccgattga aggagcaaac cagcccaggc
     4741 aacgggatgg tgaagcatga agctcgcaga agccctcatc ctgcgcgccg acaaccaacg
     4801 acgcatcgag cagctgaagc aacgcatcgt ccagaacgcc atcacccagg aaggcagcga
     4861 tccggccgag gacccgaacg cactcgttca ggagatggaa cgaaccgctg acgacctcgc
     4921 aaccctcatc cagcagatca acgcgaccaa cagcagcgcc acactcgacg acggtacgac
     4981 cctcagcgct gcactggccc gccgcgatgt cctcaagctg aagcacgcca tctaccgcga
     5041 cgccgctcag gctgcctcgc aaaccggcaa ccgttacatg cgctccgaac ttcggatgac
     5101 acctcagatc gatgtcgcga cgatccagca acgcgccgac gatctggccc gcgagcatcg
     5161 cgaactcgac acccgtatcc aggaaaccaa ctggcgcacc gacctgatcg aataacggca
     5221 agcgcggcag gagcaggtag ccgtttgaaa cagaggcgag cacaaaccct tcgctgtggg
     5281 gtaacacagc ggctgacggc gcgaggggca gtgccacaac ttcattgtca gcccagcact
     5341 gtcaccagtc accgcgcact gtaaccgtta cgaccaggtg ctgatctgtt tcgatggcga
     5401 gggcgggcac ggggatccgc cgcgcaagtt aatcgacaat cgttgaagcg actcccctgt
     5461 cgtcctgcgc cgccgcgaag gacctccccg ctttggccga tca
//
LOCUS       DASUGJ010000544         7631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1615573, whole genome shotgun sequence.
ACCESSION   DASUGJ010000544 DASUGJ010000000
VERSION     DASUGJ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615573"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..640)
                     /locus_tag="VFV93_12280"
     CDS             complement(53..640)
                     /locus_tag="VFV93_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166168.1"
                     /translation="MHRTGQTALVALGALLSWLMLGQLILVEDVGSRTTFDPRRLLLY
                     VLLLTAPMLTFVPLARWARAPFYDLEAVAGWGTLGYVVAVVTPAEPPTLAQFLLFLLP
                     LTVALATVATLGSYLVGLRFYRHDRRRYDFVRARRQGYLVALVLVACMLLFSIGTLSP
                     TSAVLLLVVAVLAEMFSLSRDSQRLRQGIERRLSS"
     gene            756..1028
                     /locus_tag="VFV93_12285"
     CDS             756..1028
                     /locus_tag="VFV93_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEX5166169.1"
                     /translation="MRSGEPLQVVIGDVVRLRKPHPCGSYDWTVVRLGADIGLRCHGC
                     GRRVLLPRRDLEKRLKTFLERGPAFAAAEAALSEPTAEQPDEAVGA"
     gene            1028..2368
                     /locus_tag="VFV93_12290"
     CDS             1028..2368
                     /locus_tag="VFV93_12290"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166170.1"
                     /translation="MPADSGSIRTVLRNQGFLRLWILQGTTQTAQNMINFALLILVRE
                     IIETQNISEANTAISLLVLAFSAPAVVFSPIAGVVVERADKRTIMIVANGLRALAVLA
                     FLALDPAWPAVLTLAALYTITFFLGAVGQFFGPAQLAAIPVIVRPRDLISANALFNLT
                     FTGSQVLGFALFGPLAIKLLGTEQTLVGILFLYIATTALATMIPSTPSSYRPKPDDDQ
                     HPFKQFAREAREGLSFVAERPLLIKSIIYLTLATTTYLMIAALGPEFIADVLGLPSED
                     IAYVVAPAGLGVVVGALLVNRVTAVIQPVRLVDLGLIGAGTSLALFALTEPVGSAIGL
                     SDGDPGKLLVGISIFFAATLGISNAFILVPSQAIMQRASPEEGVARVYATYFTISNIF
                     SFAPVLFAGAIADIVGVMRVMVAIAILLALTGVYNLRRAAPELPDDDMPLMTPS"
     gene            complement(2387..3337)
                     /gene="pyrB"
                     /locus_tag="VFV93_12295"
     CDS             complement(2387..3337)
                     /gene="pyrB"
                     /locus_tag="VFV93_12295"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX5166171.1"
                     /translation="MTQLGFPRHILHVDQFSRTDLDRLFAHARAMRGLVGRPGDERLR
                     GRILATLFFEPSTRTRLSFESAMLRLGGQVISAESADITSSAVKGETIEDTVRIVESY
                     ADAIVIRHPKSGIPDAAAAVASVPILNAGDGPGEHPTQALLDLFTIAEEIGRVDDLTI
                     TLAGDLRYGRAPRSLAMLLTRTKDCRLMLSTPPGIEMAEDVVRRLTAEGVQFDIEPDL
                     TAAASQSDVVYVTRVQKERFPSEEAYQQARGSYHFTPAHLAAMKPDSIVMHPLPRVDE
                     IAAEVDSDRRAAYFRQARYGVYIRMAILDILLGSGDGTKV"
     gene            complement(3516..4931)
                     /gene="rpoN"
                     /locus_tag="VFV93_12300"
     CDS             complement(3516..4931)
                     /gene="rpoN"
                     /locus_tag="VFV93_12300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX5166172.1"
                     /translation="MVRMDAGYEINTEMRQWISPSLIEANYILSLSRQELESVINQEL
                     DSNPALEIGDEAVCPMCGGIMEGTWCPTCMISHQQDEQRESWEDFPEQAYTAPVTRED
                     SDDFDPMTLVGGSVSLRDQIALDIATMIHDDLIPVAHYLLDSLDERGFVDCDADDVCR
                     LFEIAMDEFEEVLCAIQLLAPVGVGARDLRECLLLQITHLQGQEIAIPPYVEEIVSTY
                     LQELGAHKFGLIARELGVTTEVVEEARDFIRANLTPFPLQSQEARQWRSPVESPYVAP
                     DVVIAIKDGELVVEVVETRQAQLRVNSLYDQMARAALAARKNSNMDEETRGHVREHVA
                     RAKLFLGNVRQRHETLARISRNVAELQEDFLRGGVRKLVPLTRATVAQQVGVHESTVS
                     RATANKYVMLPNRKVIPFSDFFTPSLSVKDMIKEMIEEEKDSLTDKRIVELLNRRGVR
                     IARRTVAKYRSELNILPSTLR"
     gene            5292..6398
                     /locus_tag="VFV93_12305"
     CDS             5292..6398
                     /locus_tag="VFV93_12305"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5166173.1"
                     /translation="MKVHPTFAIVVLWCLFQWGIVAGEGAVGIIFGLAALLTVFGCVL
                     LHELAHALVAIQHGVGVRDITLLPIGGVARVEQSLLSPRSEAIIALAGPGMNILIALA
                     LTPLVAVVVAIRHLDHPISILLYANEISIAGFILYLWIANLLLAAFNLLPAFPMDGGR
                     VLRATWSRFRGKDEATQAAVLIGYVLAVLLALFGLVIGDYLMPLVSIFIVVAAQMEAN
                     FVRMESALRRLPVGQFALWEAGGIRPDAPLAHAVNGGPRDVAVTRDGAVVGMLWRHDL
                     LRHLNGAHRDLFVRDIMDRRFNAVDVNDSIYDVHLWLATSSRPAVPVVENGIYRGIFT
                     GERLAHVYEHIDRHSFSWQRNLLGVLNRLRLASR"
     gene            6573..7034
                     /locus_tag="VFV93_12310"
     CDS             6573..7034
                     /locus_tag="VFV93_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5166174.1"
                     /translation="MKVSTRGEYGMRAMVSLAREFGNGPLALSSIAHDSAVPAAYLEQ
                     LLGTLRRSGLITSTRGAHGGYALARPPEDVLIGEIYRSLEGPVAPMDCVSEVEPDELC
                     PLIDGCATRVVWLKVRDSIVEALDSTTLADLLSQSRQRRAPAVAEAGAQTV"
     gene            7074..>7631
                     /locus_tag="VFV93_12315"
     CDS             7074..>7631
                     /locus_tag="VFV93_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5166175.1"
                     /translation="MPETDIYLDHAATTPVDPRVLDVMLPFFSERYGNPSSIYTLGQD
                     ARTGLDWARGTLADLLGCRPREITMTSGATESNNLAIKGVAWWHRFNDPARKNHIVVS
                     AIEHHAVLHAADALIPHGFKVSIVQPDTDGIVRPEAVEAALRSDTCLVSVMYANNEIG
                     TIQPIREIAALAHERGITVHTDAVQA"
BASE COUNT         1308 a         2545 c         2403 g         1375 t
ORIGIN      
        1 ggcgaggctg cggccatcgc ccctacgcga caggtgttct gtgtttgact gctcaggacg
       61 acaggcggcg ctctatcccc tgacgcagcc gttggctgtc gcggctcagt gagaacatct
      121 cagcgaggac ggcaacgacc agcagcagga cggcactggt cggtgaaagc gtgccgatgc
      181 tgaataacag catgcaggcg accagcacca gcgcgaccag atagccctga cggcgggcgc
      241 ggacgaaatc gtaccgcctc cggtcgtggc ggtagaatct cagccccacc aggtaggagc
      301 cgagcgtcgc gacagtcgcc agcgcgaccg tcaacggcag caggaacaac agaaactgcg
      361 ccagtgtcgg cggctcggcc ggcgtgacga cagcgacgac gtagccgagc gttccccagc
      421 cggcgaccgc ttccaggtcg tagaacggcg ctcttgccca gcgggccaac ggcacgaacg
      481 tcagcatcgg ggcagtcagc aaaagcacgt agagcaacag ccggcgcgga tcgaatgtcg
      541 tccgcgagcc gacatcctcg accagaatca gctggccgag catcagccag ctcagcagcg
      601 cgccgagcgc aaccagtgcg gtttgtccag ttcgatgcat gtgtacgtac tccctggcaa
      661 gagtctagca gatgttccga ccgccgaccg gttcggcggc tatacttcgc cggatgacag
      721 agagaacgac cctcaatcgg ggattcagaa ctgatatgcg atcgggtgaa ccgctacagg
      781 ttgtgatagg tgatgtcgtc cgcttgcgga agccgcaccc gtgcggctcg tatgactgga
      841 ccgtcgtccg gctcggcgcg gacatcggcc tgcgctgcca cggctgcggg cgacgggtgc
      901 tgctgccgcg tcgcgatctg gagaagcgcc tgaagacctt cctggagcgc ggtccggcct
      961 tcgctgctgc cgaggctgcc ctgtcagagc caaccgccga acagcccgat gaagccgtgg
     1021 gcgcctgatg ccggctgatt ccggcagtat cagaaccgtt ctgcggaacc agggatttct
     1081 ccggctctgg atactccagg ggacgaccca gaccgcccag aacatgatca acttcgcgct
     1141 gctgattctg gtgcgcgaga tcatcgagac gcagaacatc tccgaagcca acacggccat
     1201 ctcactgctc gtgctggcat tcagtgcgcc ggccgtcgtc ttctcgccga tcgccggtgt
     1261 cgtcgtcgag cgggccgaca agcgcacgat catgatcgtc gccaacgggt tgagggcgct
     1321 ggccgtcctc gcctttcttg cgctggaccc agcctggccg gccgtcctga cactggctgc
     1381 gctctacacg atcacgttct tcctcggcgc ggtcggccag tttttcgggc cggcacagct
     1441 ggcggcgatt cccgtcatcg tccgaccgcg cgacctgatc agtgccaacg cgctcttcaa
     1501 cctgaccttc accggctccc aggtgctggg attcgcgctc ttcggaccgc tggcgatcaa
     1561 gctgctcggc accgagcaaa cgctggtcgg catcctgttt ctctacatcg caacgacggc
     1621 gctggcaacg atgatcccgt cgacaccatc gagctaccgg ccgaagccgg acgacgacca
     1681 gcaccccttc aagcagttcg cccgcgaggc gcgtgagggg ctctcgttcg tcgctgaacg
     1741 gccgctgctg atcaagtcga tcatctacct gacactggcg acgacaacct acctgatgat
     1801 cgcggccctc ggccctgagt tcatcgccga tgtcctcggc ctgccgtcgg aagacatcgc
     1861 ctatgtcgtc gcgccggccg ggctcggtgt cgtcgtcggg gcgctgctgg tcaaccgcgt
     1921 cacagcggta atccagccgg tacggctcgt cgacctcggc ctgatcggcg ccgggacgtc
     1981 gctggccctc ttcgcgctga ccgagccggt cggtagcgcg attgggctgt cggacggcga
     2041 tcccggcaag ctcctggtcg ggatcagcat cttcttcgcc gcgacgctgg gcatctcaaa
     2101 cgccttcatc ctcgttccga gccaggcgat catgcagcgt gcctcacccg aggagggcgt
     2161 cgcccgtgtc tacgcgacgt atttcacgat ctccaacatc ttctcgttcg cgccggtcct
     2221 cttcgccggc gcgatcgccg atatcgtcgg cgtcatgcgc gtgatggtcg cgatcgcgat
     2281 cctgctggca ctgaccggtg tctacaacct gcgccgcgcc gcccccgaac tgccggacga
     2341 cgacatgccg ttgatgacgc caagctgagg cacaagcgca tccacgttaa accttcgtcc
     2401 cgtcgccgga gccgagcagg atatcgagga tcgccattcg gatgtagacg ccgtagcgcg
     2461 cctggcggaa gtaggcggca cgacgatcgg aatcgacctc ggcggcgatc tcatcgacgc
     2521 gggggagtgg gtgcatgacg atggagtcag gcttcatcgc ggcaagatgc gccggggtga
     2581 agtggtagct gcctcgcgcc tgctgatacg cctcctcgct cggaaagcgc tccttctgga
     2641 cccgtgtgac gtagacgaca tcggattggg acgcggcagc agtgagatcc ggctcgatgt
     2701 cgaattggac gccctcggcc gttagacgcc tgacgacgtc ctcggccatc tcgatccccg
     2761 gcggcgtact gagcatcagc cggcagtcct tcgttcgcgt cagcagcatc gccagcgagc
     2821 gcggggcgcg gccgtagcgc aggtcgccgg ccagcgtgat ggtcaggtcg tcgacccggc
     2881 cgatctcctc ggcaatcgtg aacaggtcca gcagcgcctg cgtcggatgc tcgccggggc
     2941 cgtcgccagc attgagaatc ggaaccgagg caacggccgc cgccgcgtcg ggaataccac
     3001 tcttcggatg gcgaatgacg atcgcgtcgg cgtacgactc gacgatccgg accgtgtcct
     3061 cgatcgtctc gcctttgacg gccgacgacg tgatgtcggc gctctcggcc gagatcacct
     3121 gaccaccgag ccgtagcatg gccgactcga agctcaggcg cgttctggtg ctcggctcga
     3181 agaacagcgt cgccagaatc cgcccgcgca gccgctcgtc gcccggccgg cccaccagcc
     3241 cgcgcatcgc ccgtgcgtgc gcgaacagcc tgtccagatc ggtccggctg aactgatcca
     3301 cgtgcagtat gtgtcgagga aacccgagtt gcgtcacccg cccgtccctt cggtcgccag
     3361 tcagcaatcc gcgccgctcg ccactgatga ggatacagca acggcgggaa cgaaccagcg
     3421 ggaaaacccc tcccctggga agggaggggc ttctggccgc acgaaatcta agcccctctc
     3481 cgcttggggg agggggttgg ggaagcggtt tctgcctacc gcaacgtact cggcaggata
     3541 ttgagttccg agcggtactt cgcgacggtt cggcgggcga tgcggacgcc gcgtcgattg
     3601 aggagttcga cgatgcgctt gtcggtcagc gagtcctttt cctcctcgat catctccttg
     3661 atcatgtcct tgaccgacag cgacggcgtg aagaagtcgc tgaatgggat gaccttgcga
     3721 ttggggagca taacgtactt gttggcggtt gcccggctga cggtcgactc gtggacgccg
     3781 acctgctggg caaccgtcgc ccgcgtcagc gggacgagct tgcggacgcc gccacgcagg
     3841 aagtcctcct ggagctcggc gacattgcgg gagatgcgcg ccagcgtctc gtgccgctgg
     3901 cggacgttgc cgaggaacag cttggcacgg gcgacgtgct cgcggacatg accgcgtgtc
     3961 tcttcgtcca tgttgctgtt cttgcgggcc gccagcgccg cgcgggccat ctgatcgtag
     4021 agcgagttga cccgtagctg cgcctggcgt gtctcgacga cctcgacgac cagttcgccg
     4081 tctttgatcg cgatgacgac atccggcgcg acatacggtg actcgaccgg cgaacgccac
     4141 tgacgcgctt cctggctctg gagcgggaac ggcgtcaggt tagcgcgaat gaagtcacga
     4201 gcttcctcca ccacctcggt cgtgacgcca agctcgcggg cgatcagtcc gaacttgtgg
     4261 gcgccaagct cctgcaggta ggtgctgacg atctcctcga cgtagggcgg aatcgcgatc
     4321 tcctgccctt gcagatgggt gatctgcaac agcaggcact cacgcagatc acgggcgccg
     4381 acacccaccg gcgccagcag ctggatcgcg cagagcactt cttcgaactc atccatcgcg
     4441 atctcgaaca gccggcagac atcgtccgca tcacagtcaa cgaagccgcg ctcgtccagc
     4501 gagtccagca ggtagtgggc gaccgggatc aggtcatcgt ggatcatcgt cgcgatgtcg
     4561 agcgcgatct ggtcgcgaag gctgacgctg ccgccaacca gcgtcatcgg gtcgaagtcg
     4621 tcggagtcct cgcgggtaac cggcgccgtg taggcctgct cggggaagtc ctcccacgac
     4681 tcgcgttgct catcctgctg gtgggagatc atgcaggtgg ggcaccacgt cccttccatg
     4741 atgccgccgc acatcggaca gaccgcttcg tcaccgattt cgagcgctgg gttgctgtca
     4801 agctcctgat tgatgacgga ttcgagttcc tggcgagaca ggctgagaat gtagttcgcc
     4861 tcgatcaggc ttggcgagat ccactgccgc atctcggtgt tgatctcgta gccggcgtcc
     4921 attcgaacca tccagcgctt ccctttccct tccccggtga ggaagtctcc cgcgagacgc
     4981 gaccgttccc ggcgccagat ggcaaaccat cagtggcgcg gttcttgcgg ccctccctcg
     5041 acgccctgcg cgagatactt gatagtacac atctggatca cgtctcgcga gtcatcaggg
     5101 acgcaatccg cgtgccacgc aacatgtacg aacacctgtt cgtgactcac cggccctcgt
     5161 ctgctatgat ggcgcggtcc tggttggcgt cagcgcggct aaaggccgcg ataggtgtgt
     5221 gtacgcgaca aggaacggta cggtctcgct tggggcaatc gatccagatc ggaacgatca
     5281 ggggcatcga cgtgaaggtc cacccgacct tcgcgatcgt cgtgctctgg tgcctgttcc
     5341 agtggggaat cgtcgccggc gagggcgctg tcggcatcat cttcggcttg gcggccctgc
     5401 tgactgtttt cggctgcgtc ctgctgcacg agctggcgca cgcgctggtc gcgatacagc
     5461 acggcgtcgg cgtgcgagac atcacgttgc tgccgatcgg cggcgtcgcg cgggtcgagc
     5521 aatcgctctt atcaccacgc tctgaagcta tcatcgcgct ggccggacct gggatgaaca
     5581 tcctgatcgc gctggcgctg acgccgctgg tggccgtcgt cgtcgcgatt cgccacctcg
     5641 accacccgat cagcatcctg ctctacgcca acgaaatctc gatcgccgga ttcattctct
     5701 acctctggat cgccaacctg ctgctggccg cgttcaacct cctgcccgcc tttccgatgg
     5761 atggtggacg cgtcctgcgg gcgacctggt cccgcttccg gggcaaggac gaggcgaccc
     5821 aggcggccgt cctgatcggc tatgtgctgg cagtcctgct ggcactcttc gggctggtga
     5881 tcggcgacta tctgatgccg ctggtcagca tcttcatcgt tgtagcggcc cagatggaag
     5941 ccaatttcgt ccggatggag tcggcgttgc ggcggctgcc ggtcggccag ttcgcgctct
     6001 gggaggcggg cggtatccga cctgacgcgc cactcgcgca cgccgtcaac ggcgggccgc
     6061 gcgacgtggc cgtcacccgt gatggtgcgg tcgttgggat gctctggcgg cacgacctgc
     6121 tgcgtcatct gaacggcgcc catcgcgacc tgttcgtgcg tgacatcatg gaccggcgct
     6181 tcaacgcggt cgatgtcaac gactcgatct acgatgtcca cctctggctg gcgaccagca
     6241 gccggccggc tgtcccggtc gtcgaaaacg gcatctatcg cgggatcttc accggcgagc
     6301 gcctggccca cgtctacgag catatcgacc gccacagctt cagctggcaa cgcaatctgc
     6361 tgggcgtcct caaccggctg cgcctcgcct cacgctgatc ccaacccgtc ccggaataga
     6421 cgccgccact cgtccgttat agactggtgg tggcaagttg ggaataagtc ttactcgtag
     6481 aaccgccaca caaatgcttg acccggccgt acgaacaggt gtacgattcg taaacgtcca
     6541 gccccggcgc gatcgcgaaa ggacgccgcc gaatgaaagt ttccactcgt ggagaatatg
     6601 ggatgcgagc gatggtgtcg ctcgcccgtg agttcggcaa cggtccgctc gcgctgtcca
     6661 gcatcgcgca cgactcggca gtgccagccg cctacctgga gcagctgctc gggacgctgc
     6721 ggcgctccgg cctgatcaca tcgacacgtg gcgcacacgg cggctacgcg ctggctcgcc
     6781 cgcccgaaga cgtgctgatc ggtgagatct accgctccct cgaaggtccg gttgcgccga
     6841 tggattgcgt ctccgaggtc gagcccgacg agctctgccc gctgatcgac ggctgcgcga
     6901 cgcgcgtcgt ctggctcaag gtgcgagaca gcatcgtcga ggcgctcgac tccaccacgc
     6961 tggcagatct cctcagccag agccgccagc ggcgagcgcc agccgtggcc gaagcagggg
     7021 cgcagaccgt ctgattctca accgccacaa ccccatctgg gaggagctcg tcggtgcctg
     7081 agaccgacat ctacctcgac catgcggcga cgacgccggt cgatccgcgt gtgctcgacg
     7141 tgatgctgcc attcttcagc gaacgctatg gcaacccatc cagcatctac accctcggac
     7201 aggacgcccg caccgggctc gactgggcgc gcggcacgct ggccgacctg ctcggctgcc
     7261 ggccgcgcga gatcacgatg accagcggtg cgaccgagtc gaacaacctc gccatcaagg
     7321 gtgtcgcctg gtggcaccgc ttcaacgatc cggcccgcaa aaaccacatc gtcgtctccg
     7381 cgatcgagca tcatgccgtc ctgcacgcgg ccgatgcgct gatcccgcac ggcttcaagg
     7441 tcagcatcgt ccaaccggac accgacggca tcgtccggcc cgaagctgtt gaagcggcat
     7501 tacgttccga cacctgtctc gtctcggtca tgtacgccaa caacgagatc ggcacgatcc
     7561 agcccattcg cgagatcgcg gcgctggctc acgagcgggg catcaccgtc cacacggatg
     7621 ccgttcaggc g
//
LOCUS       DASUGJ010000545         6429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2031762, whole genome shotgun sequence.
ACCESSION   DASUGJ010000545 DASUGJ010000000
VERSION     DASUGJ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031762"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFV93_12320"
     CDS             <1..1182
                     /locus_tag="VFV93_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX5166176.1"
                     /translation="LAGPAAIFTLSDNLTMESGLAAVVAAGLVAGNAEFPHKEEAHQF
                     KGDVTALIIASVYLLLAATLEPEDLTRLGWRGPLVVVILMVVARPLAVYLSTWRSPLT
                     LRERTFVAAIGPRGVVAASLATFVTLSLRDDYPDEAASMLGLVFLTIAMTIAVQATYA
                     NWLASRLGVRPMGVLIVGGGRVGRMLAERLVATGEDVSMIEMDVEKAELARKLGVDVT
                     VGDATSTAVLEKAGIKHAHTVVATTRSDKDNLLACQLARTRYGRDHVVSRVTDPESLP
                     SFEELGIRVMNPSAATATILANLVRRPSIFGLLTGTASNDADVTEVVVTSDSVAGKAL
                     KDLSIPGDCLVMLIRREGRRMIPHGGSVLAPGDVLTIVGTDGATEKAREYFAGYGRAY
                     G"
     gene            complement(1367..1906)
                     /locus_tag="VFV93_12325"
     CDS             complement(1367..1906)
                     /locus_tag="VFV93_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166177.1"
                     /translation="MQRRGLLIGVSAVLGLCLLGCVLVFVSVIPSFRDEVRDGVHDAV
                     ATEVARQIPATTGGAAQPGAYTLTAADLEARLRETLDDEQDEQDPVVRITDTQIEIGV
                     TSKGQDAIYTGKPVAEDGKLSIQEMSTDNGIVRFVLPPDTIAGAIEDAVNTHLATNNL
                     RLDSVELTDGQLTLVVSQA"
     gene            complement(2002..4626)
                     /locus_tag="VFV93_12330"
     CDS             complement(2002..4626)
                     /locus_tag="VFV93_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5166178.1"
                     /translation="MTTPGRRPTGSRREDANGTRQPGTGALSPRRGTTRSLATVNLLL
                     ANVSDGVILLDGEGRIIDANDAAERLFGEPIEVGTDPAWFIPRYQMATPDGAPLPVDR
                     LPSLRALAGESVRSAELSITRPDGSRVYVSISASPVIVHGGIDGVLLILNDISERVRR
                     DREAAAFQTLSQQLASSELDLNNVYKTIVTRIAEITGARVVRLMLHDPVSRTLRRAAI
                     QSTRGMPEEDDGPMGLSELTLDALAARTRLPVIVPDFDEFPSGTRPTTSGAEPSPGAA
                     IAVPLIVHSELVGTLSYDLAEPHVFDDAEIAFLGMIAAHSAVAIYNATLFQQRTRERA
                     FLADIIDHLPAGLIVFDIVYPTVRGGRAAQADYRVSMLNGQALSYLPPRLVERTRSRR
                     NGIVGLRLRRIATDEQSRRLIELLDQAFLGGEIVTGEEGNFGNDGAAGEGENWWNGSI
                     VPLRDRAGRVSELVLLATDITEQVTTRRRIEELVRIAGTRAAELEATVSAMTGAVTVC
                     DATGRLRLANSASLATFGVDSLAELMRISGLEERILLRHTDGRPVQPADRPLVRALAG
                     ETLQADYTLFHHGTGRDVHRRTSAAPVRDGAGDIIGAVAVETDITSLIEIDRLKDEFF
                     SMAAHELRTPLTAIKGYVQILAKQLARNEAPSPMILKSLTTLREQGERMERLINELLD
                     FARVETGQLAFRYREFDLIALLEESVAETARATQRHWITLKTPHAVIQGRWDRDRLAQ
                     VFANLLTNAVKYSPDGGAITVAVGWSGRREDDVTISITDEGLGIPAQDLPRLFARYSR
                     VGDSSHFHRSGLGIGLYITKEIVDSHGGTISASSQAGHGSTFTVMLPLQPRRIARPAP
                     SDQPVAVAETIPAASQAN"
     gene            complement(4732..5418)
                     /locus_tag="VFV93_12335"
     CDS             complement(4732..5418)
                     /locus_tag="VFV93_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX5166179.1"
                     /translation="MLFGVDSNLDLAQALFDLGGVQFGSFDLGPTAGTSPIYINPRVL
                     ISEPSMLRRVARLIHTEIQADHTRRRPRLASFAAVAGVPMGGLHIATAYALESDTPLI
                     YLRPSRTADGETDPLIEGRIVAGQSALIIDDLMTGGRSILSTAQQLEDAGMLVRDAIV
                     LIDREQGGVERLHEHGCRVTPILRLKTMLNYYPAAELITDATFERCMTYLSDGKEADR
                     ANRETGPEAD"
     gene            complement(5710..>6429)
                     /locus_tag="VFV93_12340"
     CDS             complement(5710..>6429)
                     /locus_tag="VFV93_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEX5166180.1"
                     /translation="GHITFGDPDFLNGPTHALRICRALHEEFPRVTFDFTARIEHLLQ
                     HRDLLPEFRELGCVFVLTAVETISSHILEKLDKGHTKQDVIDALSAMDAAGITMRPSL
                     LPFTPWTTLDDYRDLLAFFAEYDLAGSVDPVHFSIRLLVPPGSALLDQPDTDDWLGPL
                     DAANFTYTWRNPDPRVDDLQRQVAALVERAARDGENPDDSFAAITALAWSITGDTPPP
                     TTPRRVRLPGESPRLTESWFC"
BASE COUNT          995 a         2019 c         2307 g         1108 t
ORIGIN      
        1 ctggccggac cggccgccat cttcacgctc tcggacaacc tgacgatgga gtcggggctg
       61 gcggcggtcg tcgcggccgg gctggtggcc ggcaacgccg agttcccaca caaggaggag
      121 gcgcaccagt tcaagggcga cgtgaccgcg ctgatcatcg cctccgtcta cctgctgctg
      181 gcggcgacgc ttgagccgga ggacctgacc cggctcggtt ggcgcggccc gctggtcgtc
      241 gtgatcctga tggtcgtcgc ccggccgctg gctgtctacc tgtcgacctg gcgttcgccg
      301 ctgacgctgc gcgagcgtac gttcgtcgcg gcgattggcc cgcgcggtgt cgtcgctgcc
      361 tcgctggcga cgtttgtgac gctgtcgctg cgcgatgact atccagatga ggccgcctcg
      421 atgctggggc tggtcttcct gacgattgcg atgacgattg ccgtccaggc gacctatgcg
      481 aattggctgg cgagccggtt aggagtgcga ccaatggggg tactgatcgt cggaggtggc
      541 cgcgtcggcc ggatgctggc cgagcggctg gtcgcgaccg gcgaggatgt ctcgatgatc
      601 gagatggatg tcgagaaggc ggagcttgcc cgcaagctcg gtgtcgatgt aacggtcggc
      661 gacgcgacca gcacggccgt tcttgagaaa gcaggcatca agcatgccca cacggttgtc
      721 gcgacgaccc gctctgacaa ggacaacctg ctggcctgcc agctcgcccg gacgcgctat
      781 gggcgagatc acgtcgtctc ccgcgtcacc gatccggagt cgctgccgag tttcgaggag
      841 ctgggcatcc gcgtcatgaa cccttctgcc gcgacggcga cgatcctcgc caacctggtc
      901 cggcgtccca gcatcttcgg gctgctgacc gggacggcct cgaacgatgc cgacgtcacc
      961 gaggtggtcg ttaccagcga cagcgtcgcc ggcaaggcgc tcaaggacct gtcgatccct
     1021 ggcgactgcc tggtgatgct gatccgccgc gagggtcgcc ggatgatccc ccacggcggc
     1081 agcgtcctgg cgcccggcga tgtcctgacg atcgtcggta ccgatggcgc aaccgagaaa
     1141 gcgcgtgagt acttcgccgg ctatggccga gcgtatgggt aatccgatcc gttacgcagc
     1201 tgaatggtgg aaacgaggac cgacccactc cccctgtcat cccgacgcag gagggatctc
     1261 gtcactcgtt ggcgctggca tggactgaac gtgaacgaga tccttcgctt tcgctcagga
     1321 tgacaggggt gcggggtgcg gtcctcattt acggcgtgtc actgggctat gcctggctga
     1381 cgaccaacgt cagctggccg tctgtcagct caacgctgtc gaggcggagg ttgttggtgg
     1441 cgagatgggt gttgacggcg tcctcgatcg cgccggcgat cgtatcgggc ggcaggacga
     1501 agcggacgat gccattgtcg gtcgacatct cctgaatgga gagcttgccg tcctcggcga
     1561 cgggcttgcc ggtgtagatg gcatcctgtc ctttcgatgt cacgccgatc tcgatctggg
     1621 tatcggtgat gcgaacgacc gggtcctgct cgtcctgctc gtcatcgagc gtctcacgca
     1681 ggcgtgcttc gaggtcggcc gcggtcagcg tgtaggcgcc gggctgggca gcgccgccgg
     1741 ttgtggccgg gatctggcgc gcgacttcgg tcgcgaccgc gtcgtggacg ccatcgcgga
     1801 cctcgtccct gaacgatggg atgacggaaa caaacaccag cacgcagccg agcaggcaga
     1861 ggcccagcac ggctgagaca ccgatcaaca gtccacgtcg ctgcacgcgg cgcactcctc
     1921 ctcaagacac aacggacgcc ggcaatggca ctggccggcg ttgcgtgacc aaggatagta
     1981 gacggcgtgg gtgtctgtcc gtcagtttgc ctggctggcc gccgggatcg tctcggcgac
     2041 ggcgaccggc tggtcgctgg gggcaggccg cgcgatccgg cggggctgga gcggcagcat
     2101 gacggtgaag gtgctgccat gtcccgcctg gctgctggcg ctgatcgtgc cgccgtggga
     2161 atcgacgatt tccttggtga tgtagaggcc gattccgagg ccgctgcggt ggaagtggct
     2221 gctgtcgccg acgcgcgaat agcgggcgaa cagccgtggc agatcctggg ccgggatacc
     2281 gagcccctca tcggtgattg agatcgtcac gtcgtcctcc cgcctgccgg accagccgac
     2341 cgcgacggtg atcgcgccgc cgtccggcga gtacttgacg gcgttggtga gcaggttggc
     2401 gaagacctgc gcgagccggt cgcggtccca gcggccctgg atgacggcat gcggcgtctt
     2461 gagtgtgatc cagtggcgct gggtggcacg ggccgtctcg gcgaccgact cttccagcaa
     2521 ggcgatcaaa tcgaattcgc ggtagcggaa cgccagctgg ccggtttcga cgcgcgcgaa
     2581 atcgaggagc tcgttgatca gtcgctccat ccgttcgccc tgttcgcgga gtgtcgtcag
     2641 cgacttgagg atcatcggcg agggcgcttc gttgcgggca agctgcttgg cgaggatctg
     2701 gacatacccc ttgatggccg tcagcggtgt tcgcagctcg tgggcggcca tcgagaagaa
     2761 ctcgtccttg aggcggtcga tctcgatcag gctggtgatg tcggtctcga ccgcgacggc
     2821 gccgatgatg tcgccggccc cgtcgcggac gggcgcggcg ctggtgcggc ggtggacatc
     2881 gcggccggtt ccgtggtgga agagggtgta gtcggcctgg agggtttcgc cggccaacgc
     2941 gcggacgagc ggccggtcgg ccggctgaac cggacggccg tcagtgtggc gcagcaggat
     3001 gcgctcttcg aggccggaga tgcgcatcag ctcggccagc gaatcgacgc cgaaggtcgc
     3061 cagcgaggcg ctgttggcca gccgcagccg tccggtcgcg tcgcagacgg tgacggcgcc
     3121 ggtcatcgcg ctgacggtcg cttccagctc ggcggcgcgg gtgccggcga tgcggaccag
     3181 ctcctcgatc cggcgacggg tcgtgacctg ctcggtgata tcggtcgcca gcagcaccag
     3241 ctcgctgacg cggccggctc gatcgcggag cgggacgatt gacccgttcc accagttctc
     3301 tccctcacca gcggcgccgt cgttgccgaa attgccttcc tcgccggtga cgatctcgcc
     3361 gccgaggaac gcctgatcga gcagctcgat cagccggcgc gactgctcat cggtggcgat
     3421 ccggcgcagc cgcaggccga cgatcccgtt tcggcggctg cgagtgcgct cgacaaggcg
     3481 cggcgggagg tagctgagtg cctgaccgtt gagcatcgag acgcggtagt cggcctgagc
     3541 cgcccggcca ccccgcacgg tcggatagac gatgtcgaag acgatcaggc cggccggtag
     3601 gtgatcgatg atgtcggcca gaaacgcgcg ctcgcgggtg cgttgctgga agagggtcgc
     3661 gttgtagatc gcaaccgccg agtgggccgc gatcatgccg aggaacgcga tctcggcgtc
     3721 gtcgaagacg tgcggctcgg ccaaatcgta cgagagggtc ccgaccaatt cactgtggac
     3781 gatcagcggg acggcgatgg ccgctcccgg cgagggctcc gcgccagagg tggttggcct
     3841 ggtgccgctg gggaactcat cgaagtccgg cacgatgacc ggcagccgcg tgcgggcggc
     3901 cagcgcatcc agcgtcagct cgctcagtcc catcggtccg tcgtcctcct ccggcatgcc
     3961 gcgggtggac tggatcgcgg cccggcggag cgtgcgcgag accggatcgt gcagcatcag
     4021 ccgcacgacc cgcgcgccgg tgatctcggc gatgcgggtg acgatcgtct tgtagacgtt
     4081 gttcagatcc agctcggagc tggcgagttg ctgcgacagc gtctggaagg cggccgcctc
     4141 acgatcacgg cggacgcgct cactgatgtc gttgaggatc agcaggacgc cgtcgatgcc
     4201 gccgtggacg atgacggggc tggcgctgat cgagacgtag acgcggctgc catccggccg
     4261 tgtaatcgac agctcggccg aacggaccga ttcgccggcc agcgcccgca ggctcggcag
     4321 ccggtcgacc ggcagcggcg cgccgtccgg cgtcgccatc tggtagcgtg gaatgaacca
     4381 ggccggatcg gtgccgactt cgatcggctc gccgaacagt cgctcggcgg cgtcgttggc
     4441 gtcgatgatc cggccttcgc catcgagcag gatgaccccg tcggagacgt tcgccagcag
     4501 gaggttgacg gtcgccaggc tgcgggtcgt tccccgacgg ggagacaacg cgccggtgcc
     4561 cggttgccgt gttccattgg catcctcgcg ccgggaccca gtgggtcgcc ggcctggtgt
     4621 cgtcatccgg cgcgcctctc ccccttcacc gttgtggcgt ccttcgtcag agggatgata
     4681 ctcggttacg gggagtagga accgcgtcga tccaggtggt cacccgccgg ctcagtcagc
     4741 ctcgggtccc gtttcccggt ttgcgcggtc ggcttccttg ccatcgctga ggtaggtcat
     4801 gcagcgctcg aaggtggcgt cggtgatcaa ttccgcagcg gggtagtagt tcagcatcgt
     4861 cttcagccgc aggatcggtg tcacccggca gccgtgctcg tgcagccgct cgacgccgcc
     4921 ctgttcgcgg tcgatcagca cgatcgcgtc gcgaaccagc atccccgcgt cttccagttg
     4981 ctgggcggtc gaaaggatcg agcgcccgcc ggtcatcagg tcgtcgatga tcagcgccga
     5041 ctggccggcg acgatccgtc cctcgatcag cggatcggtc tcgccatcgg ccgttcgcga
     5101 cggccgcagg tagatcagtg gcgtgtccga ttccagcgcg taggcggtcg cgatgtggag
     5161 cccgcccatc gggacgcccg cgacagccgc aaacgacgcc agccgcggcc ggcggcgggt
     5221 gtggtcggcc tgaatctcgg tgtggatcag ccgcgcgacg cgtcgcagca tcgatggctc
     5281 ggagatgagc acgcgcgggt tgatgtagat cggcgatgtg ccggcggtcg gcccgaggtc
     5341 gaatgacccg aactggaccc cgccgagatc gaatagtgcc tgcgccagat caaggttgga
     5401 atcaacgccg aacagcaccc tggtttccct cccgataggg cagtcagccc tcaccccccg
     5461 gccccctcgc ccaatactgg gagaggggga gatatcgcgg aggagccgcg cagcctcccc
     5521 agtcggcgcg acgtaccata atcccgacaa tctaccatat ttcggggctg cgtcgggaat
     5581 cgggacgcgg gattcgggat caatgaaccg agcacggcga tgaggattcc gtctgttacc
     5641 aacccccgaa tcccaaatcc cgagccccgc cccttacgcc ggaccaaact ggtccgcagt
     5701 tggctctgct cagcagaacc aggattctgt caggcgtggg gattcgcccg gtaggcggac
     5761 gcggcgtggt gtggttgggg gtggagtgtc accagtgatc gaccaggcga gggcggtgat
     5821 agcggcgaag ctgtcgtcgg ggttctcgcc gtcgcgggcg gcgcgttcga ccagcgcggc
     5881 gacctggcgt tggaggtcgt cgacgcgtgg atcggggttc cgccaggtgt aggtgaagtt
     5941 ggcggcgtcg agcggaccga gccagtcgtc cgtgtcgggc tgatccagca gggccgagcc
     6001 aggcgggacc agcaggcgga tcgagaagtg gacgggatcg acgctgccgg ccaggtcgta
     6061 ctcggcgaag aacgccagca ggtcgcggta gtcgtccagc gtcgtccagg gcgtgaacgg
     6121 cagcaacgac ggccgcatcg tgatcccggc ggcatccatc gcggacagcg cgtcgatgac
     6181 gtcctgcttc gtgtggccct tgtcgagctt ctcgaggatg tgggacgaga tcgtctcgac
     6241 agctgtcagg acgaagacgc agcccagttc gcggaactcc ggcagcagat cgcggtgctg
     6301 gagcaggtgc tcgatccggg ccgtgaagtc gaacgtgacg cgcgggaact cctcgtgaag
     6361 tgcgcggcag atgcgtagcg cgtgggtcgg cccgttcagg aagtccggat cgccgaaggt
     6421 gatgtggcc
//
LOCUS       DASUGJ010000546         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_504451, whole genome shotgun sequence.
ACCESSION   DASUGJ010000546 DASUGJ010000000
VERSION     DASUGJ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504451"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..>2951)
                     /locus_tag="VFV93_12345"
     CDS             complement(1..>2951)
                     /locus_tag="VFV93_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5166181.1"
                     /translation="TTVEAVVVTGSRIRHDAYSNPDPVDVVLPESAANQGVSDVARML
                     QTSTVAAGSPQVTAATSTAFVQSGGVGAETLSLRGLGANRTLVLLNGRRAGPAGTRGE
                     TSAFDFNVMPLSVIERVEILKDGASSIYGSDAVAGVVNIITKKGDGAVAEAFVSNPGE
                     SGGGESRFNASWGKSFERGNFRVTADYHREEELKQGDRDYFNCGQDYMFEPGTHRRAD
                     VVDPRTGRPACTDLPWGHVWVYDYNFTEPGNLPNFTQNNGTVQLMQYDYDGNLGQYVP
                     GLSADPGAINQMRTPPGWFPVSYDRTSDGVTNSDHPFQDGQSLIPKQEQMTLFADGEF
                     DISDSVTAYAEVLLNRRKTKTNGYRQFWSYKYTSDFNFFDAFWNYTYSTSPGSGDPQS
                     AGWTGAQYLSPTPITDHADSSIDVKYQRYVAGLRGVLKSGWDWDVSLQYSNSDGDYTN
                     DQIFDDSIRDTNITFAGFGGVPYVPQATCVGTVSSVRGAPCVDIPWFDPDFLNGNVSP
                     EVRDFLFGEETGNTEYTQWSAEAYMSGSIFELPAGSLGVAFGVHYQEDEIRDVPGDIT
                     LAGNAWNTSSAGVTAGSDTTKAVFAELEVPLLRDKPGVDNLTLTASGRYTDVESYGNG
                     DTYKVGINWSITPTVRIRASQGTSFRTPALFELYLADQTGFLRQSSIDPCIGWGAALE
                     TGDISQRIADNCAADGLAPDFPGGNITATVVTGGGLGILNAETSLSRTAGIVWQPNFA
                     DLSVSVDYFDILVRDEVDQLGAPQIVFNCYDSDFFPNDPLCDLFQRVGTDQRIDNVRD
                     SYINIAKQKNRGWDVAATFRTSLGPGSLTVETQHTFQEADDRALFANTLEELNGLVGD
                     PDWVGNLNLSYDWGPWSFFWGMNFIGSSSNEAHYFDRNPFPQTYRGEPVDIVLSTDTV
                     IYHAVSVSRTFEDSGILARLGVANLLDEEPPRVTTLDLGEVNTIGNSAFYSQYDWLGR
                     RFFLQLTKTF"
BASE COUNT          552 a          894 c          933 g          572 t
ORIGIN      
        1 ctagaaagtc ttggtcaact gcaggaagaa gcgtcgcccc agccagtcgt actgcgaata
       61 gaaggccgag ttgccgatcg tattgacctc gccaaggtcc agggtcgtca ccctgggcgg
      121 ctcctcgtcg aggagattgg caacgccgag gcgggcgagg atgccgctgt cctcgaatgt
      181 gcggctgacg gagacggcat gataaatgac cgtatcggtg ctgaggacga tgtcgacagg
      241 ttctccgcga taggtctgcg ggaacgggtt ccgatcaaaa taatgcgcct cattggagct
      301 ggagccgatg aagttcatgc cccagaagaa tgaccacggg ccccagtcgt aactcaggtt
      361 gagattgccg acccaatccg gatcaccgac gaggccgttc aattcctcga gcgtgttcgc
      421 gaacagggcg cgatcatccg cttcctggaa cgtgtgctgg gtctcaacgg tcaggctgcc
      481 cgggccaagc gaggtccgga aagtcgccgc aacgtcccat cccctgttct tctgcttggc
      541 gatgttgatg tagctgtccc ggacattgtc gatgcgctgg tcggttccaa ccctttggaa
      601 gaggtcgcac aacgggtcgt tcgggaagaa atccgagtcg tagcaattga atacgatctg
      661 cggtgcaccg agctgatcca cttcgtccct gaccaggatg tcgaagtagt cgacggacac
      721 gctcaggtcg gcgaaattcg gctgccacac gatcccggcc gttcgcgaca aggaagtctc
      781 cgcattgagg atacccaggc ctccgccggt gacgacggtt gccgtgatgt tgcctcctgg
      841 aaagtccggt gcgagaccgt ccgcggcgca gttgtccgcg atccgctgag aaatgtcccc
      901 cgtctccagc gcagcgcccc acccgatgca gggatcgatc gaggattgcc gtagaaagcc
      961 cgtctggtcg gcgagataca gctcgaacag ggccggagtg cggaacgacg tgccttggct
     1021 cgcccgaatc cgtaccgtcg gcgtgatcga ccagttgatg ccgaccttgt aagtatcacc
     1081 gttgccgtac gactcgacat cggtatatcg gcccgacgcc gtcagggtca ggttgtcgac
     1141 gcccggcttg tccctgagca gcggcacctc cagctcggca aaaaccgcct tcgtcgtgtc
     1201 actgcccgcc gtgactcccg cgctggaggt gttccacgca ttgccggcga gcgtgatgtc
     1261 cccgggaacg tcgcggatct cgtcttcctg gtagtgcacg ccgaatgcaa cgcccagcga
     1321 acccgcagga agctcgaaga tcgagccgga catgtaggct tccgcagacc actgggtgta
     1381 ttccgtgttg ccggtctcct cgccgaacag gaaatcgcga acctccgggg acacgtttcc
     1441 gttcaggaaa tccggatcga accacgggat gtccacgcaa ggcgcgccgc gcacggaaga
     1501 aacggtgccg acacaagtcg cctggggaac atagggaaca ccgccaaaac cggcgaaggt
     1561 gatattcgtg tccctgatcg aatcatcgaa aatctggtcg ttggtgtagt cgccgtccga
     1621 gttgctgtat tgcagcgaaa catcccagtc ccagccggac ttgaggacgc cgcgcagccc
     1681 ggcgacatag cgctgatact tcacgtcgat ggagctgtcg gcatgatcgg tgatcggcgt
     1741 cgggctgaga tactgcgcgc cggtccagcc ggcactctgc gggtcgcctg aacccggact
     1801 cgtcgagtag gtgtagttcc agaacgcatc aaagaaattg aagtcgctcg tgtacttgta
     1861 ggaccagaac tggcgatagc cattcgtctt cgtcttgcgg cggttcagca ggacttctgc
     1921 gtaggcggtg acgctgtccg agatgtcgaa ttcaccgtcc gcgaatagcg tcatctgttc
     1981 ctgtttcggg atcagcgact gcccgtcctg gaacgggtgg tcactgttgg tgacgccatc
     2041 cgacgtgcgg tcgtaactga cggggaacca tcccggcggc gtgcgcatct ggttgatggc
     2101 gccggggtcg gcggaaagcc cgggtacgta ctgtccgagg ttgccgtcgt agtcatattg
     2161 catcaactga acagtgccgt tgttctgggt gaagttcggc aaattgcctg gttcggtgaa
     2221 gttgtagtcg tagacccaaa cgtggcccca gggcagatcg gtgcaggcgg gtcgcccggt
     2281 gcgcgggtcg acgacgtcgg cccttcgatg ggtccccggc tcgaacatgt agtcctggcc
     2341 gcaattgaag tagtcccggt caccctgctt gagttcttcc tcccggtggt agtcggcggt
     2401 gacccgaaaa ttgcccctct cgaagctctt gccccacgag gcgttgaatc gcgattcgcc
     2461 gccaccgctt tcgcccggat tcgatacgaa ggcctctgcg accgcgccat cgcccttctt
     2521 ggtgatgata ttgacgacgc cagcgacggc gtccgaacca tagatcgagg atgcgccgtc
     2581 cttgaggatt tccacgcgct cgatgaccga gagcggcatc acgttgaagt cgaatgcgga
     2641 cgtctcaccg cgcgttcccg cgggcccggc gcggcggccg ttcaagagta cgagcgtccg
     2701 gttcgcgccc agcccccgca gcgaaagcgt ttcggcgccg acgccgccgg actgcacgaa
     2761 ggccgtcgac gtcgccgcgg tcacctgggg cgaaccggcc gcgacggtcg aggtctggag
     2821 catcctggcg acgtcgctga cgccttgatt cgccgcgctt tctggcagga cgacatcgac
     2881 cggatccggg ttcgaatagg cgtcgtggcg gatgcgcgaa cccgtcacca cgacggcttc
     2941 gacggtcgtc g
//
LOCUS       DASUGJ010000547         7331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1892388, whole genome shotgun sequence.
ACCESSION   DASUGJ010000547 DASUGJ010000000
VERSION     DASUGJ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892388"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1212
                     /locus_tag="VFV93_12350"
     CDS             271..1212
                     /locus_tag="VFV93_12350"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5166182.1"
                     /translation="MSEGENRAGRIEAEVLVVGGGPAGLSAALYLARFDRRVILFDAG
                     QGRSTWHQINHNYLGFPGGIAARDLRELGRRQLGEYPQVTCLDRKITELRRDGDWFVA
                     TGQAGEWRGRAVVVATGVVDHYPHFEGWDEYVGRSMFWCITCDGYACKGERVVVIGNT
                     NATASEAMQLCRFTDQLTLLTNSSDCAIDDEFQHRLDAAGIPLIHDKIDTVHGEGGFF
                     KELRTEAGRTIELDQLFAVQGQTPQTELIEGLGVDLSPRGYIKVDSEQKTNLPGVYAA
                     GDVTRLHSHQISTAVHEGGQAASAANYFLYPPELKDE"
     gene            1276..2355
                     /locus_tag="VFV93_12355"
     CDS             1276..2355
                     /locus_tag="VFV93_12355"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5166183.1"
                     /translation="MATSQIADALDRQLGPFIEQMLALLRIPGMAVGVVAEGATVYAR
                     GFGVASLADRRPVTAETLFHMASVTKPFVATAIMQLAEERLVGLDDPVVRHLPYFRMA
                     DERSAQITIRQMLSHTSGMPDEDDYGWERPEFDEGALERYVRSLADRPLLGAPGERFA
                     YSNIAYEVLGDLIAKVRGISFEAAIAERILRPLRMTQSTLLVRDADPSLLSRGHVVDA
                     GGEARVSAVFPYHRAHAPSSTLYSNVTEMCRWAMALLNGGELSGARVLSRESLERMWE
                     PGLVVDPERHERIGLSWFIDAYRGRRTINHGGSDTGFRSQLFLVPEAGLAAVAMSNVD
                     YVFEAQWSATMAALEIALGPSGDRS"
     gene            complement(2336..2533)
                     /locus_tag="VFV93_12360"
     CDS             complement(2336..2533)
                     /locus_tag="VFV93_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166184.1"
                     /translation="MCHTTIKSHPWAVTSVVAAIAAAAAAGTVAEVTRRALRQIAALD
                     PRASFDPYYGMVEDALNSDPR"
     gene            2772..3029
                     /locus_tag="VFV93_12365"
     CDS             2772..3029
                     /locus_tag="VFV93_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166185.1"
                     /translation="MSRRAAIWGAAALSLIFVLLLSAVLLQPALGSNGGADRDGVINA
                     DQWSEMTADDASGWDAEGHDDNDHGDHEGDEHDGHEDDDDD"
     gene            3252..4220
                     /locus_tag="VFV93_12370"
     CDS             3252..4220
                     /locus_tag="VFV93_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5166186.1"
                     /translation="MSGGGVHTHAFDTMGSTVELVLTGRTPEAADVAFTRAKALADRW
                     ERVFSRFRPESELSRLNRNAASFRPSRLLFDGIEAAIEAARLTEGRFDPTVLPALISL
                     GYDRPYARIVAAGGEVAGQPCPVEPAPCGRWREVLLDRSRKLIQLPEGVQLDLGGIAK
                     GLYADHLAGELAAWPGGMVSAGGDMRIWGEPPDGDRWIVGIEEPERPGEDIAWFEVKG
                     GGVATSGTNRRHWRLAGEDVHHLIDPATGRPVAGDLRSVTVAADTAVAAEVAATALFV
                     AGGATTSVSALTSMIWLAVGVECDGQIRMLYQSPRGHTYAASDDAA"
     gene            4195..4950
                     /locus_tag="VFV93_12375"
     CDS             4195..4950
                     /locus_tag="VFV93_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166187.1"
                     /translation="MRPATTLPERSTSDPWRYAGPAGVIVVAFSVSVLALGAALVGAS
                     LSPLTWYLARASGLTLYLLLWLSVVSGLGMTTKLLPSPGGLGGMWALHRITSELGVIL
                     LALHLLSLALDPTVALGILGVLVPFVSDVRQPWTDFGIVTAYGAIAVSASFSIRHLIG
                     WRVWRVLHVASLPLWMMALAHGIGAGSDSSSLWAGALYVTTTASVVFLVLYRLLRIGS
                     RGWGKSRPAAHVLDREEMRKRVAEYRDRCASSS"
     gene            4932..5618
                     /locus_tag="VFV93_12380"
     CDS             4932..5618
                     /locus_tag="VFV93_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166188.1"
                     /translation="MRVLVVDDEEQLAGIIGQMLERHGYAVDLAYDGLTGLDLALTGN
                     YDAVVLDRMLPGMDGVDVCHQMRAAEVSTPVLILSARRELPERVEGLDAGADDYLGKP
                     FALTELVARVRALTRRRDKPVVPDLLQAGDVTLHQQTHEVRASGRPVELSPKEYALLE
                     YLLRNAGQVLSRDQILERVWGYDAEPEGNVVDLYIHYLRRKLDRNEAPSIIQTVRGVG
                     YVIRDGEREA"
     gene            5618..6928
                     /locus_tag="VFV93_12385"
     CDS             5618..6928
                     /locus_tag="VFV93_12385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5166189.1"
                     /translation="MFRGVARRLAILNALTVIIVIALAGGITWLALRTALDDEVDNAL
                     RDRIGTYIANPALFEHASVTATPTTAEEGDEEHHGDDDDDEHDREVVGSGDTLLFVVD
                     RSGDVTFNSRGVDLEDLPVEDAIERALAGRRDSRSVTLDESGKVRVLTVPLREGGQIT
                     GAVQALRSLEEHDRELALVGWMTLLGITLGALIAVPAGLYLSRRAMIPIDDAFGRQRS
                     FVADASHELRTPLTLIRANAELSLLDPGRPLNESLANILTEVDRTDRLVDDLLLLARV
                     DAGRLELHSERHRLGSLVADAAESMRPLFEDQQTRLTVATDTPVDVWADGDRIQQVVR
                     SLLDNALKHTSGGQVDVSAGSEGRWAVVTIRDTGSGIPSEALPHIFDRFYRIDTARSR
                     AAGGTGLGLSIAKAIVEAHGGEISITSEPDRGTTVMFRLPVTNR"
     gene            complement(6915..>7331)
                     /locus_tag="VFV93_12390"
     CDS             complement(6915..>7331)
                     /locus_tag="VFV93_12390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166190.1"
                     /translation="IAVRLARRIGLINTMVFSHLPANLFLMLTPLMPSLPLAIGLLLL
                     RSLLSQMDVPTRNSYVMAVVTPAERPAAASVTAVPRSLATATSPLISGWLLGLTTFGW
                     PLIIGGGLKIAYDLLLLRMFRSVRPPEERDRSVSGS"
BASE COUNT         1208 a         2246 c         2553 g         1324 t
ORIGIN      
        1 cggacctcgc cgacaaccgg gtccggagcg ggggtggtag ctgcgggtgc gacagacatc
       61 gcggcacact cctcggctaa caaccagtcg aaccacagag cctaccgcga tttagttgga
      121 accggtgcgg ccgcagcctc gccctgcttg actcgcgtcg aacaagtgcg aggctgcggc
      181 cgtcgcccct acgcggggga gttagatggc gcgacccttg cagggcatgg cactcgttgg
      241 ggccatgtcc tgcaatcatg gggagccggt gtgtctgagg gagagaacag agcggggcgg
      301 atcgaggctg aggtgctggt cgttgggggc gggccggccg ggttgtcggc ggcgctctac
      361 ctggcgcggt tcgaccggcg tgtgatcctg ttcgacgcgg ggcaggggcg ctcgacctgg
      421 catcagatca atcacaacta cctcggcttt ccgggcggga ttgccgcccg tgacctgcgt
      481 gagctgggcc gccggcagct tggcgagtat ccacaggtga cctgcctcga ccgcaagatc
      541 accgagctgc gccgcgacgg cgactggttc gtggcgacag gtcaggccgg ggagtggcgc
      601 gggcgggcgg tcgtcgtcgc gaccggcgtc gttgaccact acccgcactt cgagggctgg
      661 gacgagtacg tcggccgcag catgttctgg tgcatcacct gcgacggcta cgcctgcaag
      721 ggcgagcgtg tggttgtgat cggcaacacg aacgcgaccg cctccgaggc gatgcaactc
      781 tgccgcttca ccgatcagct aacgctgctg accaactcca gcgactgcgc gatcgacgat
      841 gagtttcaac accggctcga cgcagccggc attccgctga tccacgacaa gatcgacacc
      901 gtccacggcg aaggcggctt cttcaaggag cttcggaccg aggcgggccg gacgatcgag
      961 ctggaccagc tcttcgccgt ccagggccag acgccgcaga cggagctgat cgagggactc
     1021 ggcgtcgatc tcagcccgcg cggttatatc aaggtcgata gcgagcagaa gaccaacctg
     1081 ccgggtgtct acgccgccgg cgacgtcacc cggctccact cccaccagat cagtacggcc
     1141 gtccatgagg gcggacaggc tgccagcgcc gccaactact tcctctaccc gccggaactc
     1201 aaggacgagt aatatccggt tgcaggctcg atcgcatctg ttcccgagga tcgacacccc
     1261 gattgaggag gcagaatggc gacgtcccag atcgctgatg cgcttgaccg gcaactgggc
     1321 ccattcatcg agcagatgct ggcgctgttg cggataccgg gtatggctgt cggcgtcgtg
     1381 gccgagggtg caacggtcta cgcgcgcggg tttggcgtcg cgagtctcgc tgatcggcgt
     1441 ccggtgacgg ctgagacgct gttccatatg gcgtcggtca cgaaaccgtt cgtcgcgacg
     1501 gcgatcatgc agctggccga ggagaggctg gtcgggctgg atgacccggt tgtccggcac
     1561 ctgccctatt tccggatggc cgacgagcga tctgcgcaga tcacgatccg ccagatgctg
     1621 tcgcacacga gtgggatgcc cgacgaggat gactacggct gggagcggcc cgagttcgac
     1681 gagggcgcgc tcgagcgtta cgtgcgctcg ctggcggatc ggccgctgct tggcgcaccc
     1741 ggcgagcgct ttgcctactc gaacatcgcc tacgaagtgc tgggcgacct gatcgcgaag
     1801 gtgcgcggaa tctcgttcga ggcggctatc gccgagcgta tcctgcggcc gctgcggatg
     1861 acacagagca cgctgcttgt gcgggatgcc gatccgtcgc tactcagccg tgggcacgtc
     1921 gttgatgccg gcggcgaggc gcgggtcagc gccgtcttcc cgtaccatcg tgcccacgcg
     1981 ccaagctcga cgctgtattc gaacgtcacc gagatgtgcc gctgggcgat ggcgctcctc
     2041 aacgggggcg aactgagtgg tgcccgggtt ctctcgcgcg agtcactgga gaggatgtgg
     2101 gagccggggc tagtcgtgga ccccgaacgg cacgagcgga tcgggctgtc gtggttcatc
     2161 gacgcctatc gtgggcgccg aaccatcaat catggcggct ccgacaccgg tttccgcagc
     2221 cagctgtttc tcgtcccgga ggccggcctg gcagccgtcg cgatgtcgaa tgtggactac
     2281 gtcttcgagg ctcaatggtc tgcgacgatg gcggcgttgg agatcgccct tggccctagc
     2341 ggggatcgga gttgagcgcg tcctcgacca tcccgtagta gggatcgaac gacgcgcgcg
     2401 ggtcgagcgc cgcgatctga cgcagcgcac ggcgcgtcac ctccgcgacg gtaccggccg
     2461 ctgccgctgc cgcgatggcc gccaccactg acgtcactgc ccagggatgc gacttgatgg
     2521 ttgtatgaca catgggccgc tctcctctcc ctggcgccgg gtcatgaccg gtcgtcgcag
     2581 gttctctcta ttttatcgga cagcgtgcct ggtttcagat tccccaaccc ccaatgggac
     2641 tggatcttcc cagctggttc ccccagacac taatccagtt ccaatcttcc accactagcg
     2701 ttgtgagcgt gcggcggatg agaacgtcgc gctggtgagt cggttcgggc gaaccgggag
     2761 gagtaacgac ggtgtcacga cgtgcggcaa tctggggcgc ggcggcgctc tcgctgatct
     2821 ttgtgctgtt gttgagcgcg gttctgctgc agccggccct cggcagcaat ggcggcgccg
     2881 accgcgatgg agtcatcaac gccgaccagt ggtccgagat gacagctgac gacgctagcg
     2941 gctgggacgc cgagggccac gacgataacg accacggtga tcatgagggt gatgagcatg
     3001 acgggcatga ggacgacgat gacgactaga cagctcacgc ggcgtgcagg tgacacgggg
     3061 gacgcagcgt gtcgagttcg ccgggctgtc ctgatcgggg tactggccag ctttgttggc
     3121 ttcttcggat tgactgttgc gacatcgccg ccgtcccaat tgacggcgag cgcggcggat
     3181 gacaacgtca aggtcgtgtg tgccgtgaat gtctgccagc cggtcagagt tcacgtgcgg
     3241 acccgtacgt catgagcggc gggggagttc acacgcacgc tttcgacacg atgggctcaa
     3301 cggttgagct ggtcctcacg ggccgaacgc ctgaagccgc ggacgttgcg ttcacgcgtg
     3361 ccaaggcgct cgccgaccgt tgggaacggg tattcagccg tttccggccc gagagtgagc
     3421 tgtccaggct gaaccgaaat gcggcgtcct tccgtccgag ccggctgctc ttcgatggta
     3481 tcgaggcagc gatcgaggcg gcgcgtttga ccgagggtcg ctttgatccg acggtcctgc
     3541 cggcgctcat cagcctcggc tacgatcgtc cgtacgcgcg aatcgtcgcc gccggtggtg
     3601 aggtggccgg gcaaccctgc ccggtcgagc cggcaccgtg cggccgctgg cgggaggttt
     3661 tgctcgaccg ttcgcgaaag ctgatccagt taccggaggg ggtccagctc gacctgggtg
     3721 gcatcgccaa gggcctctac gccgaccatt tggccggcga attggcggcc tggccgggtg
     3781 gaatggtctc ggccggcggc gacatgcgta tctggggaga accgccggat ggtgaccgct
     3841 ggatcgtcgg gattgaggaa cccgagcgcc ccggcgagga catcgcctgg ttcgaggtga
     3901 aagggggtgg cgtcgccacg agcgggacga atcgccgcca ctggcggctg gcgggggaag
     3961 atgtccacca tctgatcgat ccggcgaccg gccggccggt cgccggcgat ctgcggtcgg
     4021 tgacggttgc tgctgacacg gcggtcgccg ctgaggtcgc cgcgacggca ctcttcgtcg
     4081 ctggcggcgc cacgacatca gtgtcagcgc tgacatccat gatatggctg gccgttggcg
     4141 tcgagtgcga cggacagata cgaatgctct atcaatcacc gagaggacac acgtatgcgg
     4201 ccagcgacga cgctgcctga acgatccacg agcgatccct ggcgctacgc cggccctgcc
     4261 ggggtcatcg ttgtcgcttt ctcggtgagc gtgttggcgc tcggggcggc gctggttgga
     4321 gcttcgctat caccgctgac atggtatctg gcgcgggcat ccggattgac gctctacctg
     4381 ctgctctggc tgtcggtcgt cagcgggctg gggatgacga caaagctatt gccgtcgcca
     4441 ggcgggctgg gcgggatgtg ggcgctgcac cggattacgt ccgagcttgg cgttatcctg
     4501 ctggcgttgc atctgctgtc actggcgctc gatccaacgg ttgcgttggg tatccttggc
     4561 gtgctcgttc cgtttgtatc ggatgttcgg caaccgtgga cggacttcgg tatcgtgacc
     4621 gcatatggag cgatcgctgt cagcgccagc ttcagcatcc gccacctgat cggctggcgg
     4681 gtctggcggg tgctccatgt cgccagcctg ccgctctgga tgatggccct ggcccatggg
     4741 atcggtgctg ggtccgatag cagcagtctc tgggcaggtg cgttgtatgt tacgacgacg
     4801 gcgtcggtcg tgtttctcgt gctgtatcgc ttgctgcgga tcggctcgcg cggttggggg
     4861 aagtcccggc cggctgccca tgttctcgac cgcgaggaga tgcggaagcg tgtcgctgaa
     4921 tatcgagacc gatgcgcgtc ctcgtcgtag atgatgaaga gcagttggcc gggatcatcg
     4981 gccaaatgct ggagcggcat gggtatgccg ttgacctcgc ctacgatggc ctgaccggcc
     5041 tcgacctggc cctgaccggc aactacgatg cggtcgtcct cgaccggatg ctgccgggga
     5101 tggatggtgt cgatgtctgt caccagatgc gtgccgccga ggtctcgacg ccagtcctga
     5161 tcctgtctgc ccgccgcgag cttccggagc gggtcgaggg gctggacgcc ggcgccgacg
     5221 actatctggg gaagccgttc gcgctcaccg aactggtggc gcgcgttcgc gcgttgaccc
     5281 ggcggcgcga caagccggtc gtcccggatc tgctgcaggc tggtgatgtg acgcttcacc
     5341 aacagaccca cgaagtccgg gcctcaggcc ggccggtcga gctatcgccc aaagaatacg
     5401 cactgctgga atacctgctg cgcaacgccg gccaggtgct gagccgtgac cagatcctcg
     5461 aacgggtctg gggctacgat gccgaacccg agggtaacgt cgtcgatctc tacatccact
     5521 atctgcgccg caagctcgac cgcaacgagg cgccgtcgat cattcagacc gttcgaggtg
     5581 tcggatacgt catcagagat ggcgagcgcg aagcgtgatg tttcgcggtg tcgcgcgacg
     5641 cctcgccatc ctgaatgcgc tgaccgtcat catcgtgatc gcgctggcgg gtggtattac
     5701 ctggctggcc ctccggaccg cgctcgacga cgaggttgat aacgccctgc gggatcggat
     5761 tggtacctat atcgcaaacc cagctctgtt cgagcatgcg tctgtgactg ccacaccgac
     5821 gacggctgaa gaaggcgacg aggagcatca cggcgatgac gacgacgatg aacatgatcg
     5881 tgaggtcgtc ggatcgggtg acacgctgct cttcgtcgta gaccgaagcg gagatgtcac
     5941 cttcaactcc cgcggggttg atctggaaga cctcccggtc gaggacgcga tcgagcgggc
     6001 gctggccggc cggcgtgact cacgcagcgt gacgctcgat gaatccggaa aggttcgcgt
     6061 cctgacagta ccactgcggg aaggcggcca gatcaccggc gccgttcagg cgcttcgcag
     6121 cctcgaagag cacgatcgcg agttggcgct ggttggctgg atgacgttgc tcgggattac
     6181 gctcggcgca ctgattgccg tcccagccgg cctctatctc tcacggcggg cgatgatccc
     6241 tatcgacgac gcgttcggac gccagcgttc gttcgtcgcc gacgcgtcac acgagttgcg
     6301 aacgccgctg acgctgatcc gcgccaacgc tgaactgtcg ctgctcgacc cggggaggcc
     6361 gctcaacgag tcgctcgcca acatcctgac tgaggttgat cgaaccgacc ggttggttga
     6421 tgacctgctg ctgctggcca gggtcgacgc cgggcgactg gaactgcact ccgagcggca
     6481 tcgccttggc agtctggtgg cagacgcggc ggaaagcatg cggccgctct tcgaggatca
     6541 gcaaacgcga ctgactgttg cgaccgatac ccctgtcgac gtctgggcgg acggcgatcg
     6601 catccagcag gtggttcgca gcctgctcga caacgcgttg aagcacacca gcggcgggca
     6661 ggtcgatgtc tcggccggca gcgagggccg ctgggcggta gtcacgatcc gcgatacggg
     6721 aagcggcatt ccatccgagg cgctgcctca catcttcgat cgcttctatc gtattgatac
     6781 ggcgcggagc cgggcggccg gcggaaccgg gctcggcctg tcgatcgcga aggcgattgt
     6841 cgaggcgcac gggggagaga tctcgatcac gagcgaaccg gatcgtggca cgacggtgat
     6901 gttccgactg ccggttacga accgctaacc gatctgtcac gctcttcggg cgggcggact
     6961 gagcggaaca tccggagcag gagcaggtcg taggcgatct tgaggccgcc gccgatgatg
     7021 agcggccagc cgaaggtcgt cagaccgagc agccagccgg agatcagcgg gctggtggcc
     7081 gtcgccaggc tacgcggaac ggccgtgacg ctggccgcag ccggtcgctc ggcaggcgtg
     7141 acgacggcca tcacgtacga gttgcgggtt gggacgtcca tctgcgagag caggctgcgc
     7201 aggagcagca gcccgatcgc cagcggcagg cttggcatca gtggcgtcag catcaggaag
     7261 aggttggcgg gcaggtgcga gaagaccatc gtgttgatca ggccgatccg ccgcgccagc
     7321 cggacggcga t
//
LOCUS       DASUGJ010000548         6304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1754800, whole genome shotgun sequence.
ACCESSION   DASUGJ010000548 DASUGJ010000000
VERSION     DASUGJ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754800"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFV93_12395"
     CDS             <1..298
                     /locus_tag="VFV93_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX5166191.1"
                     /translation="IEGRIRQLEDMLKRVEVIDADAAAKSGGVVRLGTTVTIEIDGAE
                     ETYTIVGSVESNPLEGRISDKSPWGQALLGSRAGQTVNIQTPAAVLKARVVKIG"
     gene            308..973
                     /locus_tag="VFV93_12400"
     CDS             308..973
                     /locus_tag="VFV93_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5166192.1"
                     /translation="MLSLAEIAAFAHQRSAGHDTAHDALHLARVVANADGLVTDERAE
                     GAEVDEFVVEAACWLHDVVQVPKGSGPAGEAARQSADIAREFLETHQVGRRRIDAIAH
                     AIEAHSYSGGLRPQTIEAAVVQDADRLDALGAVGIARLWVTGVSLGGTLYHPTDPGGR
                     SRRLDDRAFGLDHIERKLLQLPATMNTAAGRAEATRRAEFVRVYRDEFLRELNAATES
                     NPT"
     gene            970..1437
                     /locus_tag="VFV93_12405"
     CDS             970..1437
                     /locus_tag="VFV93_12405"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX5166193.1"
                     /translation="MTEASQLEQLIATGEIDAELVEPGVPTPTVPDAARALGVEDRQI
                     VKSLLFAAKSGEVVLAILSGASKVSRQRLRDVTGLRGLELADPAVVLERTGYPAGGTP
                     PVGHSSRLDVVIDSGVMDLPVVYGGGGRIDSLLRIRPDEIARVTDARIAPIAT"
     gene            1661..2131
                     /locus_tag="VFV93_12410"
     CDS             1661..2131
                     /locus_tag="VFV93_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166194.1"
                     /translation="MSLANDSMPTVVPPLTIPGRGDRMPDLSGLSPSGAQISTRDYYM
                     RRNLAVLFICAETECADWIAAAAAVRKAAQAEDGEILLVLNGTTDTDGLPAIIDADGS
                     LAARCGLGEFDNPALFVLDRYGTIFVSNRGPNAEPDLRPRDIPNWLEFIACRCS"
     gene            2204..3778
                     /locus_tag="VFV93_12415"
     CDS             2204..3778
                     /locus_tag="VFV93_12415"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5166195.1"
                     /translation="MAAIPTQDIDAVFADWDRDDSPGCALAVVREGEIAYARGYGMSN
                     LEHGVPITPGSIFHVASISKQFTDMCIALLASEGALSLDDDIRQYVPEIPDYGETITI
                     RHLIHHTSGLRDMWSLLRMAGWRADDLINETDMLWVASRQAAPNFRPGDEYLYSNTGY
                     ALLALIIHRVSGKTLRQFAHERIFEPLGMTSTHVHDDHTEIVKGRTQAYEPRKGGGLR
                     ISIPVFDVAGTTSLFTTVEDMARWDDNFHQHTVGGAAILEQMQTPGRLNDGGPMTYGF
                     GLNIHSYRGLPLVEHSGADAGYRAQYLHFPEERLAVVCLCNLSTMTPRTLALAVADIM
                     LGDQFPEPVYDETLEPGRSDASLAGVYRNEDTGDLLRITSDDEGLAIGFGHLQRLERL
                     RGGTFRIEGQRLTRLRFEELDGRRALVFGALYSPQPDPVFMRVAEPELTEPRPFDGGE
                     FHSDEVDATYRLQPDGDALRMTHYRLDEKTLQHAYDDVYASDDLRLELERDVHGSVAG
                     FRASTGRVRGVWFARR"
     gene            3848..4963
                     /locus_tag="VFV93_12420"
     CDS             3848..4963
                     /locus_tag="VFV93_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5166196.1"
                     /translation="MTSESANAVYRARMERAKAEMARQGVDFLFVTPSSDLTYLLGYP
                     AHASERMTLLCVPREGEPFVVAPTLEASRLDSRREIVDVHRWEETDSPFELVARLVEN
                     AEGAAIGISDQTWAVFLLRLQQALPDTSFTSGNTVLRELRMVKDAQEIELLREAGVRA
                     DRAWERFIASSRIAGRTERDVADEIRGLMSDEGLEPGGFLIVGGGPNSASPHHMTSDR
                     VIQEGDSVVFDFGSPYQHYYSDITRTVHVGEPSDEYRLVYDTVLAANRAARAAVRPGV
                     ACEAIDRAARDVIEGAGYGEYFIHRVGHGLGLDGHEEPYMVEGNSLPLVPGMVFSDEP
                     GIYIPGRFGVRIEDILVCTDDGAESFNDATRDLIVMH"
     gene            4986..6143
                     /locus_tag="VFV93_12425"
     CDS             4986..6143
                     /locus_tag="VFV93_12425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5166197.1"
                     /translation="MAQLPATAEVVIIGAGIMGCSIAWHLAERGVTDVVVLERDAIGR
                     GATADAAGGIRLQFSTETNIRLSQISLDYWEHFEERFDAAINFRQQGYLFLLTDESEA
                     ETFRTNLALQQSLGVPARWVTPAEIAELNPAVLLDDVIGGTFCPKDGWADTSTSTMGF
                     AQAARRLGVKIFEETPVTGIDIEGGRISGVRSGDARIAAPLVICCAGPQTNAVGQLAG
                     LDIPILPYRRMSFITEPFDAIPPTVPMTIEFTRSLYFHPESHGFLFGMSNKDEPTSDN
                     KAVDEDWMAATVEALIERAPVFEDASVLRGWAGFYEVTPDDNPLLGRVPDLDGFIVAA
                     GFSGHGFMQGPAIGLAIAELVIDGQTKTVDLSAFRPSRFQEGALLQEHNVI"
BASE COUNT         1092 a         2024 c         2126 g         1062 t
ORIGIN      
        1 gatcgaaggc cgcattcgcc agctggaaga catgctcaag cgcgtcgaag tcatcgacgc
       61 ggatgccgcc gccaagtccg gtggtgtcgt tcggctcggg acgaccgtca cgatcgagat
      121 cgacggcgcg gaagagacat acacgatcgt cggctcggta gagtcgaatc cgctcgaagg
      181 acgcatctcc gacaagtcac cctggggcca ggcgctgctc ggctcgcggg ccggccaaac
      241 ggtcaacatt cagacgccgg cggctgtgct gaaggcgcgc gtcgtcaaga tcggttaggg
      301 cagccggttg ctatcgctgg cagagatcgc ggccttcgcc catcagcgca gcgccggtca
      361 tgacaccgcc cacgatgcgc tgcatctggc ccgcgtcgtc gcgaacgcag atgggctggt
      421 gacagacgag cgtgcggaag gagctgaggt cgacgagttc gtcgtcgaag cggcctgctg
      481 gctacacgat gttgtccagg tgccaaaagg tagcggcccc gctggtgaag cagcacgcca
      541 gtcggcggac atcgcacgcg agtttcttga gacgcaccag gtcggccgca ggcggatcga
      601 cgcgattgca cacgcgatcg aggcccactc atactcgggc gggctgcgac cgcagacgat
      661 cgaggccgcg gtcgtgcagg acgccgaccg gctggatgcg ctcggcgcgg tcgggatcgc
      721 ccggctctgg gtcaccggcg tcagcctcgg cgggacgctg tatcacccga ccgatcccgg
      781 cgggagatcg cggcggctgg acgaccgcgc ctttgggctg gaccatatcg agcgcaagct
      841 gctgcagttg ccggcaacga tgaacaccgc cgccgggcgg gccgaggcga cgcgacgggc
      901 ggagttcgtc cgcgtctacc gcgacgagtt cctgcgcgaa ctgaacgccg caacggagag
      961 caatcccacg tgacagaagc gagccaactg gagcaattga tcgcgaccgg cgagatcgac
     1021 gccgaactgg tcgagcccgg cgtgccgacg ccgaccgttc cggacgccgc ccgcgcgctg
     1081 ggtgtcgagg atcgccagat cgtcaagtcg ctattgttcg ccgcgaagtc cggcgaggtt
     1141 gtcctggcga tcctgtccgg cgcgtcgaag gtcagccgcc agcggctccg cgatgttacc
     1201 ggcctgcgcg ggttggaact ggcggatccg gctgttgtgc tggaacggac aggctatccg
     1261 gctggcggca ccccgccggt cggacatagc agccggctcg acgttgtcat cgacagcggc
     1321 gtcatggact tgccggtcgt ctacggtggt ggcggccgga ttgattcgct gctgagaatc
     1381 cgcccagacg agatcgcgcg ggtgacggac gcgcggatcg cgccgattgc cacctgacgg
     1441 aaacggaata taatccgaat acggcatgtt tctggtgata tcggcgacga gacgctgtca
     1501 gcccgccctc cgacgctggt gggccggagt tcatggcgca atccttgctg acaggacggg
     1561 ctatcgaggc aggggcgaac gtgccgggca tcgcctcatt acagacgccg cgagtgtcgt
     1621 cggcctgaat tcacctgtat gcgcgcgaag ggagggccgg atgagccttg ctaatgactc
     1681 gatgccgact gtcgttccac cgctgaccat ccccggccgc ggcgatcgaa tgccggacct
     1741 gagcggcctg tcgcccagcg gtgcgcagat cagcacccgc gactactata tgcgacgcaa
     1801 cctggccgtc ctgttcatct gcgccgagac cgagtgcgcc gactggatcg cagccgcagc
     1861 agccgtccgg aaggctgctc aggccgagga cggcgagatc ctgctggtgc tgaacggcac
     1921 gaccgacacc gacggtctgc ccgccatcat cgacgcggac ggatcgctgg ccgcgcgctg
     1981 cggcctcggt gagtttgaca acccggcgct gttcgtcctc gaccgctacg ggacgatctt
     2041 cgtctccaac cgcggcccaa acgccgagcc cgatctgcgc ccgcgagaca tcccgaactg
     2101 gctcgaattc atcgcctgcc gctgctcgta acgccggggc ggcccctgtc atcaatgtgc
     2161 gatgatgcct gccgcgacgg gatgagacgg ggcaggaggg gtgatggcgg cgataccgac
     2221 tcaggacatc gatgcggtct tcgcggattg ggaccgcgac gactcgccgg ggtgtgcgct
     2281 ggcggtcgtg cgtgagggtg agattgcgta cgcgcgcggg tacgggatgt cgaatctgga
     2341 acacggtgtt cccatcacgc ccggctcgat ctttcacgtc gcgtcgatct cgaagcagtt
     2401 caccgacatg tgtatcgcac tgctggcgag cgagggcgcg ctgtcactcg atgatgacat
     2461 tcggcagtac gttccagaga ttcccgacta tggcgagacg atcacgatcc ggcacctgat
     2521 ccatcacacg agcggcctgc gcgacatgtg gagcctgcta cggatggcgg gttggcgagc
     2581 cgacgacctg atcaacgaga cggatatgct ctgggtcgcc tcgcggcagg ctgcgccgaa
     2641 tttccggccc ggcgacgagt atctgtacag caacaccggc tacgcgctgc tggcgctgat
     2701 catccaccgc gtcagtggta agacattgcg ccagttcgcc cacgagcgga tcttcgagcc
     2761 gctgggcatg accagcacgc acgtccacga cgatcacacc gagatcgtca agggacgtac
     2821 ccaagcgtat gagccacgca agggtggcgg gctgcgaatc agtatccccg tcttcgacgt
     2881 ggccggaacg accagcctgt tcacgaccgt cgaggacatg gcgcgctggg acgacaactt
     2941 ccatcaacac acggtcggtg gcgcggcgat cctcgaacag atgcagacgc ccggccggct
     3001 caacgacggc ggcccgatga cctatggttt cggcctgaac atccacagct atcgcggact
     3061 gccgctcgtc gagcacagtg gcgccgacgc cggctaccgg gcgcagtacc tgcacttccc
     3121 ggaagagcgg ctagccgtcg tctgtctctg caacctgagc acgatgacgc cgcgcacgct
     3181 cgcgctggcg gtcgcagaca tcatgcttgg ggaccagttt ccggagccgg tctacgacga
     3241 gacgttggag cccggcaggt ccgacgcatc gctggccggc gtctatcgca acgaggacac
     3301 tggcgacctg ttgcgtatca cgagtgacga tgagggactg gcgatcggtt tcggccacct
     3361 ccaacgcctg gagcgattgc gcggcggaac gttccgaatc gagggtcagc ggttaacgcg
     3421 gttgcgcttt gaggagctcg acggcaggcg ggctctggtg ttcggcgcgt tgtattcgcc
     3481 gcaaccggac ccggtgttca tgcgggtcgc agagccggaa ctgaccgagc ctcgcccgtt
     3541 cgatggcggt gagttccaca gcgacgaggt cgatgcaacc tatcgactgc agccggatgg
     3601 tgacgcgctg cggatgacgc actaccggct ggacgagaag acgcttcagc acgcgtacga
     3661 cgacgtgtac gcgtccgacg acctgcggct tgaactcgaa cgcgacgtac acggttcagt
     3721 ggctggcttc cgcgccagca ccggccgggt ccgtggcgtc tggttcgcgc gacgctgaac
     3781 gttggcgcgg gcgggttgct agaatgcctt ggagaatcag tcggccaggc agcgaggatg
     3841 gggaatgatg acgagcgaga gcgccaacgc ggtctaccgg gccaggatgg agcgggcgaa
     3901 ggccgagatg gcacggcagg gcgtcgactt cctgttcgtg acgccgtcat ccgacttgac
     3961 ctacctgctg ggttacccgg cccatgcctc ggagcggatg acgctgctgt gtgtcccgcg
     4021 cgaaggcgag ccgttcgtcg tcgcgccgac gctggaggcc agccgtcttg acagccgccg
     4081 cgagatcgtc gatgtccacc gctgggagga gaccgattcg ccgttcgagc tggtggcacg
     4141 gctcgtcgag aacgccgagg gcgccgcgat cggcatcagc gatcagacgt gggccgtctt
     4201 cctgctgcgc ctgcaacagg cattgcccga cacgtcgttc acctccggca acacggtcct
     4261 gcgcgaactg cggatggtca aggacgcgca ggagatcgag ttgctgcgtg aggccggcgt
     4321 gcgggccgac cgggcctggg aacgcttcat cgcctccagc cggatcgcgg gccggaccga
     4381 gcgtgacgtc gccgacgaga tccgtggcct gatgtccgac gaggggctgg agccaggcgg
     4441 cttcctgatc gtcggcggcg ggccgaactc ggcttccccg caccacatga cctccgaccg
     4501 cgttattcag gaaggcgact cagtcgtctt cgacttcggc tcgccctacc agcactacta
     4561 ctcggacatc acccggacgg tccatgtcgg cgagccgtcc gacgagtacc ggctcgtcta
     4621 cgacaccgtc ctggcggcca atcgcgcagc gcgggctgcg gtccggcccg gcgttgcctg
     4681 cgaggcgatc gaccgggcgg cccgtgatgt catcgagggg gccggatacg gcgagtattt
     4741 catccaccgc gtcggtcacg ggctcgggct cgatggtcac gaagagccct acatggtcga
     4801 gggcaacagc ctgccgctcg tgccggggat ggtcttctcc gatgagccgg gcatctacat
     4861 tccgggccgg ttcggcgtcc ggatcgagga catcctcgtc tgcacggacg acggcgccga
     4921 gagcttcaac gacgcgacgc gcgatctgat cgttatgcac tagctgctgg caggggtaga
     4981 cattcatggc acagcttccg gcgaccgccg aggtcgtcat cattggcgcg ggcatcatgg
     5041 gctgctcgat cgcctggcat ctggccgagc gaggcgtgac ggacgtcgtc gtcctggagc
     5101 gtgacgcaat cggacggggc gcgacagccg acgcggccgg cggcatccgc ctccagttct
     5161 cgaccgagac gaacatccgc ctctcacaga tcagtctcga ctactgggag catttcgagg
     5221 agcgcttcga cgcggcgatc aacttccggc agcagggcta tctgttcctc ctgaccgacg
     5281 aatcggaagc cgagacgttc cggaccaacc tcgccctgca gcagtcgctc ggcgtcccgg
     5341 cccgctgggt gacgccggct gagatcgccg aactcaaccc tgccgtcctg ctggacgatg
     5401 tcatcggcgg caccttttgc ccgaaggacg gctgggccga cacatcgacc agcacgatgg
     5461 gtttcgccca ggcggctcga cggctcggcg tgaagatctt cgaggagacg ccggtaaccg
     5521 gtatcgacat cgagggtggt cggatcagcg gcgttcgctc cggcgacgcg cggatcgcgg
     5581 cgccactggt gatctgctgt gcggggccgc agacgaacgc ggtcgggcaa ctcgccggcc
     5641 ttgatattcc gatcctgccc taccggcgga tgtcgttcat caccgaaccg ttcgacgcga
     5701 tcccgccgac ggtgccgatg acgattgagt tcacccgctc gctgtatttc cacccggaaa
     5761 gtcacggctt cctgttcggg atgagcaaca aggacgaacc cacttccgac aacaaggctg
     5821 tcgatgagga ctggatggcg gccaccgttg aggcgctgat cgaacgggca ccggtcttcg
     5881 aggacgcatc ggtcctgcgc ggctgggccg gcttctacga ggtcacgcca gacgacaacc
     5941 cgctgctcgg ccgcgtgcct gacctggacg gcttcatcgt cgcagccggc ttctccggcc
     6001 atggattcat gcagggacca gccatcggcc tcgccatcgc cgaactggtc atcgacggcc
     6061 agacgaagac cgtcgatctc tccgccttcc gccccagccg cttccaagag ggcgcgttgt
     6121 tgcaggagca taatgtgatc tgagtttgcg ggactcggga ttcggggttc gggattcgga
     6181 agctgagaat gtttggcatt ggccacctgc tcccgttccc cgaatcccga atcccgagtc
     6241 ccggaggaac agtggtcatc ggcgtcggct acgtcacatt gcatgtcttc gagagccatt
     6301 cgct
//
LOCUS       DASUGJ010000549        14373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2033069, whole genome shotgun sequence.
ACCESSION   DASUGJ010000549 DASUGJ010000000
VERSION     DASUGJ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14373
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033069"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFV93_12430"
     CDS             complement(<1..495)
                     /locus_tag="VFV93_12430"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX5166198.1"
                     /translation="MPDRSGHEDVHRDAIVIDATCPLLNSHPHLVDWWIEGGVTAAAP
                     TVGGFDGAGGTLRNLARWNRLIEQDDRLMLVRSAEDVETTKQDGRFGIIYHFQGTAPV
                     EDDLDLVPLYKQLGVGIIQLAYNVKNRVGDGCEERTDAGLSYFGVDLIARLNREKVIV
                     DCSHT"
     gene            614..1378
                     /locus_tag="VFV93_12435"
     CDS             614..1378
                     /locus_tag="VFV93_12435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5166199.1"
                     /translation="MLTFEAGRSTPAIRALFPRAGHVWRRSYAVLDGSCRGRMLTDDP
                     DNPTWAAVQEYSDDSVLFLGGVVSPELVATLIEIMRRGRTLCFGAPDGDPAFDLFPLE
                     RDYDDFDVDFENRDPHLDLVPLCQPPPDLRLERIDADLLPRCAWGPWMTAGMETALNQ
                     GLGYCLLDGDVVVSEAFAGPVVEGALEMATITADAWQGRGLSTVVSARTVLECERNGW
                     TTWWNTSRTNVASQSVARKLGYRSERPYRLLAWWAT"
     gene            complement(1597..2193)
                     /locus_tag="VFV93_12440"
     CDS             complement(1597..2193)
                     /locus_tag="VFV93_12440"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5166200.1"
                     /translation="MAAAPAERRRLDRLTLTQRVVAVADNALIVAGIGNAGFDLATAG
                     DRPENFYMLGSMGLGVPIGHGLALAQPERPVIVLEGEGSVLMNLGALATVGREMAENL
                     TIVIWDNEQWQLTGGQRLATASTCDLATVARGCGIENVTTPADDESFESDIRRALDSP
                     GPHILVVKIDATPGVGRPHSDPIWHKYRFMQAVGVVPD"
     gene            complement(2174..2701)
                     /locus_tag="VFV93_12445"
     CDS             complement(2174..2701)
                     /locus_tag="VFV93_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166201.1"
                     /translation="MTDIQWHNEIIGAFKANNVRMIAHVADTVLAPMISAMEEDSFFR
                     VVTLSREEEGIGLLTGAYLGGIRGALLLQASGLGNTINAFGSLALPYQIPFVVLISQR
                     GSYYEHNIVQIAGGKASTGILHALDIQNFDLVRPDEVAFVAERGMRHAFVSRKPVALT
                     ITTQLSGGKSGSRTS"
     gene            complement(2789..3997)
                     /locus_tag="VFV93_12450"
     CDS             complement(2789..3997)
                     /locus_tag="VFV93_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5166202.1"
                     /translation="MAETVTKTLPSLLSERAQRSGPARLGNPRDSASRISFLAGFPDP
                     GSLPKEDITESTRVALEKDGEWALQYGSSRGYPGLIDEMVIKLARDQGIEATPDNILI
                     TNGASQALALIVDLLVDPGDVVVSEAPTWSGAVHNFKAAGAEVRTIPIDDKGLDVDAL
                     ERELNGLRAEGKRAKLLYFIPNFQNPTGVTTTLERRQKIADLAREHNLPIVEDDAYFD
                     LRYAGTKLPTVYSLDHAGLVMYLGTFSKIMAAGMRLGWCVADPEVISRLTGLKFEGGT
                     SPFAGHVAAEFCSSGTLVEHVGELRSLYLKRRDAMLTALEQHMPSGASWTTPEGGFFV
                     WLTLPEGASVAKLLPAAREQNVEFLPGAATYHHGEGDNQLRLSYSFADEAQIANGIRI
                     LGELVREQVA"
     gene            4188..5219
                     /locus_tag="VFV93_12455"
     CDS             4188..5219
                     /locus_tag="VFV93_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5166203.1"
                     /translation="MAIPEDELYDITIIGGGPVGLFAAFYAGLRGARTKVIEALEELG
                     GALTAIYPEKYIYDIAGFPKILAKDYVEGAVAQAMLMHPTICLKTEVTTFDRDEESGV
                     FVLGTATGEMHYSRSIVISAGIGAFEPKKIEVTGIERFEGGSGVHYFAKRTEDFRDKH
                     VVIVGGGDSAVDWANTLQPIAKSVRVVHRSKFRAHEHAIQQMADGGVQLHYPDYGIKA
                     VHGDDRLSAVTYHHLKEGHEDTVECDELIVAIGFVADLGPLKTWGFEIQRNTIVVDPL
                     TMATNVPGVYGCGDIVTYPAKFKLIATGVAEAVTAVNHCVTFYDPSARLDPGHSTNIM
                     EKREKAAGS"
     gene            complement(5243..6892)
                     /gene="ggt"
                     /locus_tag="VFV93_12460"
     CDS             complement(5243..6892)
                     /gene="ggt"
                     /locus_tag="VFV93_12460"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5166204.1"
                     /translation="MSGLSSEQVMTRELQGDVLAAAGGPPRDLTMARRGMAASVHPFV
                     SDTAIEVMRSGGNAIDAAVAAAAMLMVVEPRNGHLGGDTFILFNDAATGRVVALNGSG
                     AAPQAATADDYRARGGFPEDGLLTATVPGTLHCWTTALAQYGTKPLAKLLQPAIWYAR
                     HGVPVTSRLHRMLSLDAPTWRKFPDTARALLVDGEVPPIGGLFRQPLLADTLRRIAED
                     GADDFYRGMLAREMVAASAVNGGLFSEEDFASHQTDRREPIRIDYRGYTVYEQPPVSQ
                     GIIVLLALNILKQFDLQQYGAGSPEVVHLQIEALKLAFADRFRHLGDPAFVSIPLDDL
                     LSDEHAARQAARIDMHAVRQHGLAPTHPDTTYMCVADGNGTMVSYIHSLFSGCGVALG
                     NTGVLMNSRLLGFNLDEGDPNCLAPGKRPIHTLNNYLVEKDGQPVLVGGTPGAHWQVQ
                     TNLQMLVNTLEFGMDPQAAIEAPRFIIGDQLQVGDPQVKLESRYGEATIDALRRIGHE
                     VEIAGPWGSAGSVQLIARDPATGLLSGATEVRRAGTSVLGF"
     gene            7116..7847
                     /locus_tag="VFV93_12465"
     CDS             7116..7847
                     /locus_tag="VFV93_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166205.1"
                     /translation="MTNSISSANGVADQTVGSRILVVDDEPAISELITTALRFIGFEV
                     ASAAAGHEALATAAAFSPNLVILDVMLPDVDGFEVLRRLRGGGDRVPVIFLTARDEHE
                     NKMRGFTAGGDDYLTKPFSLEELVARVRAILRRSGAGRAEQPATRYRYADLEMDDEGR
                     RVWRGGEVVNLSPTEYRLLRYLLLNAGRVLSRDQILDHVWQYDYIGEAGVVETYISYL
                     RKKIDDREPKLIQTVRGFGYALRTE"
     gene            7860..9281
                     /locus_tag="VFV93_12470"
     CDS             7860..9281
                     /locus_tag="VFV93_12470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5166206.1"
                     /translation="MSIKARLIITISLAIALVFVVIGVVVVSATENRMILRVDQNLRA
                     APRIPPRTWGDGDRGNPFLDSRRETATLLMDADGDVRRSEPSGYASDPDPLPDVTLQD
                     LLAHAGGTFTTGSVDRSGHEYRVIVHRLEGGFLVQAVPLDNVEATVNSLIRIIVFSGI
                     VVLVVVILAVWVSVNRGLRPIEDMIGTAGLIAGGDLSHRIEHIDEATEVGRLGRALNA
                     MLGRIESSFAARQRSEQRLRQFIADASHELRTPLTSIRGYAELFRSGAASDPETLERV
                     MLRIESESERMSKLVEDLLLLARLDQGRPLEQAPVDLAALIDDVVMDARAIEPQRPIS
                     ATMAPDAIVLGDADRLRQVIVNLVTNARVHTDPETAIHVSVQTEGDEIRLSVADDGPG
                     MADDDVARVFDRFYRVDASRARSSGGSGLGLSIVASIIEAHGGRVHLDSELGRGTTVT
                     LTLPRLDRAGHAEMIDAVAVARR"
     gene            9420..10100
                     /locus_tag="VFV93_12475"
     CDS             9420..10100
                     /locus_tag="VFV93_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166207.1"
                     /translation="MRVLVVEDDQRILDFIRKGLAEEGHAVDVAQDGDEALDWPAVVD
                     FDAIVLDVMLPGRDGLEVCRTLRARGVRTPILMLTARDTVEDRVAGLDSGADDYLVKP
                     FAFAELLARLRALMRREPALLGTTLEVADLTLDTATRQVSRAGQPVMLTNKEYALLEY
                     LMRHPNQVLTRTMIAEHVWNFDFDTGTNVIDVHIRYLRRKIDDPYDLKLIQTVRGAGY
                     RISADPAS"
     gene            10097..11527
                     /locus_tag="VFV93_12480"
     CDS             10097..11527
                     /locus_tag="VFV93_12480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5166208.1"
                     /translation="MTGWLSRRGIRIRLTLWYLLVLAAVLTLFGGGVYFALRESLDDG
                     LEASIRSRSDALIGVIQVETGTPVLPASVVDVQPIPDDDGDFDDDIKDEQFARTWDRS
                     GNVISDAGGANAAVPARRSVVLPVIGGTERWARLPGEDDSFQILIRPIVVDGEVIGAL
                     EVGQSHEDIDDTLGALLIILTVAMPITLVLAGIGGLLIAGRALAPVDSVTRLARQISA
                     EDLSGRLNLDLPNDELGRLARTFDEMIARLEGAFRRQRQFTGDASHELRTPLTALKGQ
                     VEVALARPRDPAEYQAVLASVNEEVDRLIQLVGSLLTLARADAGEIELLREPISLGDA
                     VEGVIEQLEPLATQRGIRLIEEPGPAVTIDADESLVLPLLLNLVDNAIKYTPDGGDVR
                     VGWSADSGSAFVAVADSGIGIDAEHLPHVFDRFYRVDKARSRSEGGTGLGLAISRWIV
                     EAHGGEISVTSTPGLGSVFTVRLPLS"
     gene            11695..12420
                     /locus_tag="VFV93_12485"
     CDS             11695..12420
                     /locus_tag="VFV93_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5166209.1"
                     /translation="MLTERESRAAAWPAPLSWIVAVMLALVLPLSFVAAGASVLPGDV
                     AISRAVQGNLPGILDPLIVAANTLGSTPSMLAIAAVVALGLFLKGYRQPGAVVVAATL
                     AQAANVALKLTLESPRPTSSLVHVSEQTTGYGFPSGHTMGTTVMAFILVYVASTTMAA
                     GFRRRSVQAGLLLMPLLVGVSRIETGAHWPSDVLGAWLWGALVGIAIITLSRPSWSAV
                     RFPAIRSSRPVSSSIAIGDLSGD"
     gene            12496..13200
                     /locus_tag="VFV93_12490"
     CDS             12496..13200
                     /locus_tag="VFV93_12490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5166210.1"
                     /translation="MSVTTQVLTEEIEIPPAKRNAAPLNAYLARPDGIGQQPAVVIIH
                     ELFGLNDNIRDIARRFADEGYVALAVDLFSGGGNRRLCILRVMSALFLRPLKNKGISD
                     LRHAIDWLQRRPEVDPNRVGAIGFCMGGGYALALACVESDLRASSVFYCVNPRPLSAL
                     KRACPIVGSYPEDDWSARSGRKLDRALDDYRVPHDVKIYPSAGHSFFNDTLEGHNPAA
                     SADAWQRTLAFFGEHM"
     gene            complement(13290..14267)
                     /locus_tag="VFV93_12495"
     CDS             complement(13290..14267)
                     /locus_tag="VFV93_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX5166211.1"
                     /translation="MYHPTTRVLTVLELLQSQGHVTGPDLAERLEVNVRTARRYVTML
                     QDLGIPVEAERGRHGGYRLRPGFKLPPLMLTDEEALAVTLGLLLARRMGMTATAPASE
                     GALAKIERVLPDALREQVQAVQQMLAMAVSPSDAASTELLAALSLAAHRGRSVRLRYA
                     GRQREETDRVIDPYGVAFYAPRWYVAGWCHLRTDLRLFRLDRIQEITTIEHRFKPPRE
                     FDTLGFVAQSLASMPGAWTVEILLHVPIESAAQIIPRTHGTLTPVEGGTLYRRTTDNL
                     DWFARGLVRLEMPLTVISPPELKTRLRVLAREVERMAGVGNRESGIGAG"
BASE COUNT         2299 a         4636 c         4896 g         2542 t
ORIGIN      
        1 ggtgtgcgag cagtcgacga tgaccttctc acggttcagt cgcgcgatca gatcgacgcc
       61 gaagtagctg aggccggcgt cggtgcgctc ctcgcagccg tcgccgaccc ggttcttgac
      121 gttgtaggcg agctggatga tgccgacgcc gagttgcttg tagaggggga cgaggtcgag
      181 gtcgtcctcg accggcgccg tcccctggaa gtggtagatg atgccgaatc ggccgtcctg
      241 cttggtcgtc tcgacgtcct ccgccgagcg gaccagcatc agccggtcgt cctgctcgat
      301 cagccggttc cagcgagcga ggttccgtaa cgtgccaccc gcgccgtcga agccgccgac
      361 ggttggggcg gccgccgtca cgccaccctc gatccaccag tcaacgaggt gtgggtgaga
      421 gttcagcagc gggcaggtcg cgtcgatgac gatcgcgtcg cggtgaacgt cctcgtggcc
      481 agatctgtca ggcataccat ctccctctcg ctggctttca gttgacaccg atgggctgta
      541 cgatgtggca catcacccgg aaccgtcaag ttgggtcgcg ccgccgtgtc ggcggtgaat
      601 gggaggcgta gcgttgctga cgttcgaggc aggtcgaagc accccggcga tccgtgcgtt
      661 gtttccccgc gcgggtcacg tctggcgccg gagttacgcg gtgctggatg gctcgtgccg
      721 tggccggatg ctcaccgacg atcccgacaa tccaacctgg gccgctgtgc aggagtactc
      781 cgatgacagc gtgctcttcc tgggtggtgt cgtgagcccg gagctggttg cgacactgat
      841 cgagatcatg cggcgcggac ggacgctgtg ctttggcgcg cccgatggtg atccggcctt
      901 cgatctcttc ccgctcgaac gcgactacga cgacttcgac gtcgacttcg agaatcgtga
      961 cccgcatctg gatcttgtgc cgctgtgcca gccgcctcca gatctgcggc tggaacggat
     1021 cgatgctgac ctgctgcccc gctgtgcctg gggaccgtgg atgacggctg ggatggagac
     1081 ggcgctcaac caggggcttg gctattgcct gctcgacggc gacgtggtcg tctccgaagc
     1141 gttcgctggt cctgtcgtcg agggggcgct cgaaatggcg acgatcactg ccgacgcctg
     1201 gcagggacgc gggctatcga cagttgtctc agcgcggacc gttctggaat gcgagcggaa
     1261 cggctggacc acctggtgga acacatcacg aaccaacgtc gcatcgcaat ccgtcgctcg
     1321 caagctcggc taccggtccg aacgtccgta tcggctgctg gcgtggtggg cgacgtgagg
     1381 caatacggac gaggcgtgag ggccggaatc gtcgttgatc tggcgtccag tgccatgtgc
     1441 ctcgttcctc accaccattt caacgacgga acggcctcgc gcctcgacct gcgtgggtgc
     1501 aactcctcct gaccgcccca cggagcgaca gcccggccag ccaggccggg ctgtccttcg
     1561 attgtcatcg cttccatgcg ttgatgcccg atgaactcag tcaggtacga cgccaaccgc
     1621 ctgcatgaag cggtacttgt gccagatggg gtcgctgtgc gggcggccga cgccgggggt
     1681 tgcgtcgatc ttgacgacga ggatgtgcgg gccgggggag tcgagcgcgc ggcggatgtc
     1741 ggactcgaag gactcatcgt cggccggtgt cgtgacgttc tcgatgccgc agcctcgcgc
     1801 gacggtggcc aggtcgcagg tgctggccgt cgcgagccgc tggccgccgg tcagttgcca
     1861 ctgctcgttg tcccagatga cgatcgtcag attctcggcc atctcgcggc caaccgtcgc
     1921 cagcgcgccg aggttcatca ggaccgagcc ttcgccctcg aggacgatca ccggacgctc
     1981 gggctgggcc agcgccaggc cgtggccgat cgggacaccg aggcccatgc tgccgagcat
     2041 gtagaagttc tccggccggt cgccggccgt cgccaggtcg aagcccgcgt tgccgatccc
     2101 ggccacgatc agcgcgttgt cggcgacggc gacgacgcgc tgggtcagcg tcagccggtc
     2161 gagccggcgt cgttcagctg gtgcggctgc cactcttgcc tcctgaaagc tgggtcgtaa
     2221 tcgtcagcgc gaccggcttg cgcgagacga acgcgtgccg cattccgcgc tcagcgacga
     2281 aggcaacttc gtcggggcga acgagatcga agttttggat gtccagcgcg tgcaggatgc
     2341 ccgtcgatgc cttgccgccg gcgatctgga cgatgttgtg ctcgtagtag ctgccgcgtt
     2401 gcgagatcag gacgacgaac ggaatctggt acggcagcgc cagcgagccg aaggcgttga
     2461 tcgtgttgcc cagccccgac gcctgcaaca gtagcgcccc gcgaatcccg cccaggtagg
     2521 ctccggtcag cagcccgatg ccctcttcct cgcgcgatag tgtcacgacc cggaagaacg
     2581 agtcctcctc cattgcgctg atcatcggcg ccagcacggt gtcagctacg tgcgcgatca
     2641 tccggacgtt gttggccttg aacgcgccga tgatctcgtt atgccactgg atgtcggtca
     2701 tgtgtcctcc gtcgggaatc gggattcggg gcgttcgaga ggaatatgcc ccttcgacac
     2761 ccctgtgtcc cgaatcccga ttcccgccct acgctacctg ctccctcacc aactccccta
     2821 aaatcctgat gccgttggcg atctgggctt cgtcggcgaa ggagtaggag aggcgcagct
     2881 gattgtcgcc ttcgccgtgg tgataggtgg cggcgccggg caggaactcg acgttctgct
     2941 cgcgagcggc cggcagcagc ttcgcgacgc tagcgccctc cggcagcgtc agccagacga
     3001 agaagccgcc ctccggtgtc gtccacgaag cgccggacgg catgtgctgt tcaagcgcgg
     3061 tcagcatcgc gtcgcgtcgc ttgaggtaga gcgaacgcaa ctcgccgaca tgctcgacca
     3121 gcgtgccgga tgagcagaac tcggccgcga cgtgaccggc gaatgggctg gtcccaccct
     3181 cgaacttcag gcctgtgagc cggctgatga cctcgggatc ggcgacgcac cagccgagcc
     3241 gcattccggc cgccatgatc ttcgagaagg tgccgaggta catgaccaac cctgcgtggt
     3301 cgagcgagta gacggtcggc aacttcgtcc cggcgtagcg caggtcgaag taggcgtcgt
     3361 cctcgacgat cggcaggttg tgctcgcggg ccaggtcggc gatcttctgg cggcgctcca
     3421 gcgtcgtcgt gacgccggtc gggttctgga agttcgggat gaagtagagt agcttggcgc
     3481 gcttgccctc ggcccgaagc ccgttcagct cccgctccag cgcgtcgaca tcgaggccct
     3541 tgtcgtcgat cgggatcgtc cggacctcgg cgccggccgc cttgaagttg tggaccgcgc
     3601 cagaccatgt cggcgcttcc gagacgacga cgtctccggg atcgaccagc aggtcgacga
     3661 tcagcgccag cgcctgtgag gcgccgttgg tgatcaggat gttgtcgggt gtcgcctcga
     3721 ttccctgatc gcgggccagc ttgatgacca tctcgtcgat cagccccgga tagccgcgcg
     3781 acgagccgta ttgcagcgcc cattcgccgt ccttctccag ggcgacgcgg gtggactcgg
     3841 tgatgtcctc cttcggcagg ctgccgggat ccggaaagcc ggccaggaac gagatgcgtg
     3901 aggcggagtc gcgcgggttg ccgagccggg ccgggccgct acgctgcgcc cgctccgaga
     3961 ggagtgacgg gagtgtcttc gtgacagtct ctgccatgtg ggtacggatt cctttcggtc
     4021 ggtgtcgcgg ccgtggatcg gtgccttgcc cgcattgtaa ccccgtctgg cgactcacga
     4081 actgcgatgt tctgcggatg aggacaggca aaccgggccg gtatactttg gctggcgccc
     4141 agatcgcgcc ggaccggcga tttggccagg acaggggaca gtcgagcgtg gcgataccag
     4201 aggacgagct gtatgacatc acgatcatcg gcggcgggcc ggtcggcctg tttgccgcct
     4261 tctacgccgg tctgcgcggc gcgcggacga aggtcatcga ggcgctggag gaactcggcg
     4321 gcgcgctgac ggcgatctat cccgagaagt acatctacga catcgccggc ttcccgaaga
     4381 ttctcgctaa ggactacgtc gagggcgccg tcgcccaggc gatgctgatg cacccgacga
     4441 tctgcctcaa gaccgaggtg acgacctttg accgcgacga ggaatccggc gtcttcgtgc
     4501 tcgggacggc gaccggcgag atgcactaca gccgctcgat cgtcatcagt gccgggatcg
     4561 gcgccttcga gccgaagaag atcgaagtga ccggcatcga gcgcttcgag ggcggcagtg
     4621 gcgtccacta cttcgccaaa cggaccgagg acttccgcga caagcatgtc gtcatcgtcg
     4681 gcggcggcga ctcggctgtc gactgggcca acacgctcca gccgatcgcg aaatccgtcc
     4741 gcgtcgtcca ccgctcgaag ttccgcgccc acgagcacgc cattcagcag atggccgacg
     4801 gcggcgtcca gcttcactac cccgactacg gcatcaaggc cgtccacggc gacgaccggc
     4861 tctcagccgt gacctatcac catctgaaag aggggcacga agacacggtc gaatgcgatg
     4921 agttgatcgt tgcgatcggg ttcgtggccg acctcggccc gctcaagacc tggggcttcg
     4981 agattcagcg gaacacgatc gtcgtcgatc cgctgacgat ggcgacgaac gttcccggcg
     5041 tctacggctg cggcgacatc gtcacttacc cggccaagtt caagctgatc gcgaccggcg
     5101 tcgccgaagc cgtcacagcc gtcaaccact gcgtcacctt ctacgacccc agcgcccgcc
     5161 tcgaccccgg ccactcgacc aacatcatgg agaagcgcga gaaggcagcg gggagctagc
     5221 agtacgcagc acttttcact cgtcagaacc ccagcaccga ggttcctgcc cggcggacct
     5281 cggtcgcgcc ggagagcagg cccgtcgccg ggtcgcgggc gatcagttgg accgagccgg
     5341 ccgagcccca ggggccggcg atctcgacct cgtggccgat tcggcgcagg gcgtcgattg
     5401 tggcttcgcc gtagcgggat tcgagcttga cctgtgggtc gccgacctgg agctggtcgc
     5461 cgatgatgaa gcgcggtgct tcgatcgcgg cctgcgggtc catcccgaac tcgagcgtgt
     5521 tgaccagcat ctgcaggttg gtctggacct gccagtgggc gcccggtgtg ccgccgacca
     5581 gcaccggctg gccgtccttc tcgaccaggt agttgttgag ggtgtggatt ggccgcttgc
     5641 ctggcgcgag gcagttcggg tcgccttcgt cgaggttgaa gcccagcagg cggctgttca
     5701 tcaggacgcc ggtgttcccc agcgcgacgc cgcagccgga aaagagtgag tggatgtacg
     5761 agaccatcgt cccgttgcca tccgcgacgc acatataggt cgtgtcgggg tgagtcgggg
     5821 cgaggccgtg ctggcggacg gcgtgcatgt cgatccgggc cgcctggcgg gccgcgtgct
     5881 cgtcggagag caggtcgtcg agcgggatgc tgacgaaggc tgggtcgccg aggtggcgga
     5941 atcggtcggc gaaggcaagc ttgagcgcct cgatctggag gtggacgact tcaggtgatc
     6001 ctgctccgta ctgttgcaga tcgaactgct tgaggatgtt caacgccagc aggacgatga
     6061 tgccctgtga gacgggcggc tgctcgtaaa cggtatagcc gcgatagtcg atccggatcg
     6121 gctcgcgccg gtcggtctgg tggctggcga agtcctcttc gctgaagagt ccgccgttga
     6181 cggccgaggc cgcgaccatc tcgcgcgcca gcatgccgcg gtagaagtcg tcagcgccgt
     6241 cctcggcgat ccggcgcagc gtgtcggcca gcagcggctg gcggaagagg ccgccgatgg
     6301 gtgggacctc gccgtccacg agcaacgcgc gggcggtatc agggaacttg cgccaggttg
     6361 gcgcgtcgag cgagagcatc cggtgcagcc ggctggtgac cgggacgccg tggcgggcgt
     6421 accagatggc tggttgcagc aacttggcca gtggcttcgt gccgtattgc gccagcgccg
     6481 tcgtccagca atgcagcgtg ccggggacgg ttgcggtcag caggccgtcc tcgggaaagc
     6541 cgccacgggc gcggtagtcg tcagcggtcg ccgcctgcgg ggccgcgccg ctgccgttga
     6601 gggcgacgac ccgaccggtc gcagcatcgt tgaaaagtat gaaggtgtcg ccgccgagat
     6661 ggccgttgcg cggctcgaca accatcagca tcgccgccgc tgcgacggcc gcatctatcg
     6721 cgttgccgcc ggagcgcatg acctcaatcg ccgtgtcgct gacgaacggg tggacagacg
     6781 cggccatccc acgccgcgcc atcgtcaggt cgcgtggcgg cccgccggcg gctgccagca
     6841 catcgccctg caactcccgc gtcatcacct gctcgctgct caaccctgac atcgctcgct
     6901 cctctgccgt gcgtgcattc gttttcctgg tagtttcgca gacgttcccc cgtttgcgaa
     6961 accggtcgta tgtatttgct cccaggtgtc agcacgatgg cctggcaacg ctggtcctgt
     7021 tcattgccgg tcgattcgca ggaaactcac agaatacagt gcttcaatgc tgctacgaca
     7081 accgacacgt accgaacaag gagtccccgg tgagtatgac aaactccata tccagtgcga
     7141 acggcgtcgc ggaccagaca gttgggagcc gtatcctcgt cgtcgatgat gagccggcca
     7201 tatcggagct gatcacgacg gcgctgcgct tcatcgggtt cgaggtcgcc tcggctgcgg
     7261 ccggccacga ggcgctcgcg acggcggcag ccttctcgcc caacctggtc atcctggatg
     7321 tcatgctacc tgatgtcgat ggcttcgagg tgctgcggcg actgcgcggg ggcggcgacc
     7381 gcgtgccggt catcttcctg accgcccgcg acgagcacga gaacaagatg cgcggcttca
     7441 cggccggtgg cgacgactac ctgaccaagc cgttcagcct tgaggagctg gttgcccgcg
     7501 ttcgcgcgat tctgcgacgg tcaggagccg gccgggccga gcagccggcc acccgttacc
     7561 gctacgccga tctggagatg gacgacgagg gccggcgggt ctggcgtggg ggtgaggtcg
     7621 tcaatctctc gccgaccgag taccggctgc tgcgctacct gctgctgaac gccgggcggg
     7681 tcctgtcccg cgatcagatc ctcgaccacg tctggcagta tgactacatt ggcgaggccg
     7741 gcgtcgttga aacctacatc agctacctgc gcaagaagat cgatgaccgc gagccgaagc
     7801 tgattcagac ggtccggggt ttcggttacg cgttgcggac cgagtagagc ggcacgaaca
     7861 tgtcgatcaa ggcgcgcctc atcatcacga tcagtctggc gatcgcgctc gtcttcgtcg
     7921 tgatcggcgt cgtcgtcgtc agcgcgaccg agaatcggat gattctgcgc gtcgatcaga
     7981 acttgcgggc ggcgccacgc atcccgccgc gaacctgggg cgacggtgac cggggcaacc
     8041 cgttcctgga ctcacggcgt gagacggcaa cactgctgat ggatgctgac ggcgacgtgc
     8101 gccggtccga gccgtccgga tacgcaagtg accccgaccc gctgccggac gtcactttgc
     8161 aggatctgct ggctcacgcc ggcgggacct tcacgactgg ctcggtcgat cgttcagggc
     8221 atgaataccg cgtgatcgtc caccggctgg agggcggctt cctcgtccag gcggtcccgc
     8281 tcgacaacgt cgaggcgacc gtcaacagcc tgatccggat catcgtcttc tctggcatcg
     8341 tcgttctcgt cgtcgtcatc ctcgccgtct gggtgagtgt caatcgcggg ctgcggccga
     8401 tcgaggacat gatcgggacg gccggcctga tcgccggcgg tgatctgtcg caccggatcg
     8461 agcacatcga cgaggcgacc gaggtcggcc ggctggggag ggcgctgaac gcgatgctgg
     8521 gccggatcga gtcgtcgttc gcggcccggc agcgctccga acagcgcctg cggcagttca
     8581 tcgccgacgc gtcgcacgag ctgcggacac cgctgacctc gatccgtggc tacgcggagc
     8641 tgttccgcag tggagccgca tccgaccccg agacgctcga acgggtcatg ctgcggatcg
     8701 agtcggagag cgagcggatg agcaagctgg tcgaggacct gctactgctg gcccgcctcg
     8761 atcaggggcg accgctggag caggctccgg tcgatctggc cgcgctgatc gatgatgtcg
     8821 tgatggacgc ccgcgcgatc gagccgcagc ggccgatctc cgccacgatg gctcccgacg
     8881 cgatcgtgct gggtgacgcc gaccgcttgc gtcaggtgat cgtcaacctc gtcaccaatg
     8941 cacgggtcca caccgacccg gagacggcga tccacgtctc cgtccagacg gaaggcgacg
     9001 agatccgtct gagcgtggcc gatgacgggc cgggaatggc cgatgatgac gtcgccaggg
     9061 tgttcgatcg cttctaccgg gtggatgcct cgcgggctcg gtcgagcggc ggctccgggc
     9121 tgggactgtc gatcgtcgcc tcgatcatcg aagctcacgg cgggcgggtg catctcgatt
     9181 cggagctggg gcggggaacg accgtcacgc tgacacttcc ccgtctcgac agggctggtc
     9241 atgcggagat gattgacgct gtagctgtgg cgcgacggta agtgctggcg cgaaccccct
     9301 cccccaaccc cctaagcgga gagggggttg ggggaggggg ttcacgtctt aatcccgtcc
     9361 tcatctccac cagctatcat cggttgcgag atttgtgaac ggggtgggag acgctggcga
     9421 tgcgcgtact cgtcgttgag gacgatcaac gaattctgga tttcattcgc aaggggctcg
     9481 cagaagaggg gcacgccgtc gatgtcgcgc aggacggcga cgaggcgctc gactggccgg
     9541 cggtcgtcga tttcgacgcg attgtcctcg atgtgatgct gccgggtcgt gacggactcg
     9601 aggtctgccg gacgttgcga gcgcgtggcg tgcggacgcc gattctgatg ctgacggccc
     9661 gggacacggt cgaggaccgt gttgccgggc tggacagtgg cgcggatgac tatctcgtca
     9721 agccgttcgc cttcgccgag ctgctggcgc ggctacgggc gctgatgcgg cgcgagccgg
     9781 ccctgctcgg gacgacgctg gaggtcgctg acctgacgct tgatacggcc acgcggcagg
     9841 tgtcgcgtgc gggccagccg gtgatgctga ccaacaagga atacgcgctg ctggagtacc
     9901 tgatgcggca tccgaaccag gtcctgacgc gaacgatgat cgcagagcat gtctggaact
     9961 tcgatttcga caccggcacg aacgtgatcg atgtccacat ccgctacctg cgccgcaaga
    10021 tcgatgatcc ctacgatctc aagttgattc agacggtacg cggtgcgggc tatcgcatct
    10081 cggctgatcc ggcctcgtga ccggctggct ctcccggcgt gggattcgga tccggctgac
    10141 gctctggtac ctgctggtgc tggcagctgt cctgaccttg ttcgggggag gcgtctactt
    10201 cgcgctgcgt gagtcgctcg atgacggcct cgaagcgtcg atccgcagcc ggtcggacgc
    10261 gctgatcggc gtcatccagg ttgagaccgg cacaccggtt ctgccggcct cggtcgtcga
    10321 tgttcagccg atcccggatg acgacggcga ttttgacgac gacatcaaag acgagcagtt
    10381 cgcgcgaacc tgggaccgct ccggcaatgt catctctgac gccggcggcg cgaacgccgc
    10441 cgtgccggcc cgccgcagtg tcgtcctgcc ggtgatcggc gggaccgaac gctgggcgcg
    10501 gttgcctggg gaagacgaca gcttccagat cctgatccgc ccgatcgtcg tcgatggcga
    10561 agtcatcggg gcgcttgagg tcggccagtc gcatgaggac atcgacgaca cgctcggcgc
    10621 gctactgatc attctgacgg tcgcgatgcc gatcacactc gtcctggccg ggatcggcgg
    10681 gctgttgatc gccggccgag cgctggcgcc ggttgacagc gtcacgcggc tggcgcggca
    10741 gatctcggcc gaggacctca gcgggcggct gaacctcgac ttgcccaacg atgagcttgg
    10801 gcggctggcg cgaacctttg acgagatgat cgcgcggctt gagggcgcgt tccgccgcca
    10861 gcgacagttc accggcgacg cctcgcacga gctacggacg ccgttgacgg cgttgaaggg
    10921 acaggtcgag gtcgcgctgg cccgtccccg cgacccggct gagtatcagg cggtcctcgc
    10981 cagcgtcaat gaggaagtcg atcggctgat ccagctggtc ggcagtctct tgacgctggc
    11041 gcgggctgac gctggcgaaa tcgagctact gcgcgagccg atatcgctcg gcgatgccgt
    11101 cgaaggcgtc atcgagcagc tggagccgct ggcgacgcaa cgcgggatcc ggctgatcga
    11161 ggagccgggg ccggccgtca cgatcgacgc cgacgagtcg ctggttctgc cactgttgct
    11221 gaatctggtc gacaacgcga tcaagtacac gcccgacggt ggtgacgttc gcgtcggctg
    11281 gtcggccgat tccggctcgg cgttcgtggc ggtcgccgac agcgggatcg gcatcgacgc
    11341 cgagcatctg ccgcacgtct tcgatcgctt ctatcgggtc gacaaggccc gcagtcgatc
    11401 ggagggcggg accggcctcg gcctggcgat cagccgctgg atcgtcgagg cgcacggcgg
    11461 cgagatcagc gtaacttcga caccgggcct gggctcggtc ttcaccgttc ggctgccgtt
    11521 gtcgtgacgt gcccatccgc gcctcatctg gatttaatcc aacgggcgca gactgtagac
    11581 aggcagacgt aacagcaacc gaccgccggg agtgggcccg gcttcgctgg agctgtcgcc
    11641 gctagcacga caccaatcat cggcctgagg gggaactaca tggatcgtcc tggagtgttg
    11701 actgaacggg agtcccgcgc cgcagcctgg ccggcgcccc tgagctggat cgtcgcggtg
    11761 atgctcgcgc tggtgttgcc gctctcgttc gtcgcggccg gagcgagtgt cctgcccggc
    11821 gacgtggcca tctcgcgggc ggttcagggg aatctgccgg ggatacttga cccactgatc
    11881 gttgccgcca acacgcttgg ctcgacaccc agcatgctcg ctatcgcggc cgtcgttgcg
    11941 ctcgggctgt tcctcaaggg ctatcgccag cctggcgccg tcgtcgtcgc ggcaacgctg
    12001 gcccaggcgg ccaatgtcgc gctgaagctg acgctggaga gtccccggcc aaccagcagc
    12061 ctcgtccatg tctccgagca gacgaccggc tacgggttcc cgagcggcca tacgatgggc
    12121 acgaccgtga tggcgttcat cctcgtctat gtcgcgtcga cgacgatggc cgcgggcttc
    12181 cgccgtcgct ccgttcaggc cggcctgctg ctgatgccat tgctggtggg cgtctcacga
    12241 atcgagaccg gcgcgcactg gccatcggat gtcctcggcg cctggctctg gggcgcgctg
    12301 gtggggatcg cgatcatcac gctgtcccga ccgtcctggt cggcggtccg cttcccggcc
    12361 atccgttcgt cccgcccggt ttccagctca atcgcgattg gcgacctgag cggcgactga
    12421 ctcgtgacac ggcccccgca cctgatgtag cctgccggta tcgcaaccga cgagagcatc
    12481 agtgcggggg tagccatgtc cgtgacgaca caggtcctga ccgaggagat cgagatcccg
    12541 cccgcgaagc gcaacgccgc gccgctgaac gcctacctgg cgcggccgga cggcatcggc
    12601 cagcagccgg ccgtcgtcat catccacgag ctgttcgggc tgaacgacaa catccgcgac
    12661 atcgcgcgtc ggtttgcgga cgaaggctat gtcgcgctgg ccgtcgacct gttctccggc
    12721 ggcggcaacc ggcgcctctg catcctgcgg gtcatgtcgg cactctttct gcggcccctg
    12781 aagaacaagg gcatcagcga cctgcgccat gcgatcgact ggctccaacg gcgtcccgag
    12841 gttgacccga accgggtcgg cgcgatcggc ttctgcatgg gcggcggcta cgcgctggcg
    12901 ctggcctgtg tcgagagcga cctgcgcgcc tcgtccgtct tctactgcgt caacccgcgc
    12961 ccgctctcgg ccctcaagcg cgcctgtcca atcgtcggct cgtaccccga ggacgactgg
    13021 tcggcccgct ccggccgcaa actcgaccgc gccctcgacg actaccgagt cccccacgac
    13081 gtcaagatct atcccagcgc cggccactcg ttcttcaacg acaccctcga aggccacaac
    13141 cccgccgctt ccgccgacgc ctggcagcgg acgttggcgt tctttgggga gcatatgtga
    13201 gggcgtcggg actcgggaat cgggattcgg ggctggataa cgcgccattc gtctgtcgag
    13261 atcgtgcgat gcttaacaga tatgcggcct cacccagccc cgattcccga ttcccgattc
    13321 ccgacgcccg ccatccgctc cacctcccgg gccagcacgc gcagccgcgt cttcaattcg
    13381 ggtgggctga tgactgtcag cggcatttcg aggcggacga gaccgcgggc gaaccagtcg
    13441 agattgtcgg tggtgcggcg gtagagggtg ccgccttcga ccggtgtcag cgtaccgtgg
    13501 gtgcggggga tgatctgcgc ggccgactcg atcgggacgt gcaggaggat ctcgaccgtc
    13561 caggcaccag gcatcgatgc cagcgactgg gcgacgaagc cgagcgtgtc gaactcgcgg
    13621 ggcggcttga agcggtgctc gatcgtcgtg atctcctgga tgcggtcgag ccggaagagg
    13681 cgcaggtcgg tccgcaggtg gcaccagccg gcgacgtacc agcgtggcgc gtagaaggcg
    13741 acgccatacg gatcgatgac ccggtcggtc tcctcgcgct ggcgtcccgc atagcgcagc
    13801 cggaccgatc gcccccggtg ggccgccagc gagagcgccg ccagcagttc ggtcgaggcg
    13861 gcgtcggatg gcgagacggc catcgccagc atctgctgga cggcctgaac ctgctcgcgg
    13921 agggcatcag gcaggacgcg ctcgatcttc gccagcgcgc cctcgctggc cggggcggtt
    13981 gccgtcatcc ccatccgccg tgccagcagc agtccgagcg tcaccgccag cgcctcttcg
    14041 tcggtcagca tcagcggcgg cagcttgaag ccgggccgca gccggtagcc gccgtgccgg
    14101 ccccgttcgg cctcgacggg aatgcccaga tcctgcaaca tcgtgacata acgacgagcg
    14161 gttcggacgt tgacttctag ccgttcggcc aggtccggcc cggtcacatg cccctgcgat
    14221 tgcagcaatt cgaggacagt caggacgcgc gtcgttgggt gatacatgac cgcattccat
    14281 gagataataa cagggtaatc aggaagacgc gaacgaggcg gaagaagggg gcaggcaagt
    14341 ggcgacgatg acgcttcgag agtcgatcca gtc
//
LOCUS       DASUGJ010000550         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1755720, whole genome shotgun sequence.
ACCESSION   DASUGJ010000550 DASUGJ010000000
VERSION     DASUGJ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755720"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFV93_12500"
     CDS             <1..672
                     /locus_tag="VFV93_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5166212.1"
                     /translation="VGGPLLTDCYAIIVPNQGAVMIDAPRDAWQAAIEAAEAQNARTL
                     LAIATHGHWDHITDMARVREQGIPVAANLADANLFADPHGQGLTLPFAVDPVTIDRPL
                     NEGDRLAIFNYELHVLHTPGHTQGSVTIWVPAIDALFTGDTLLKGGAGHTAEPGASLP
                     ALATSVRRLAAFPESTTLYPGHGAPTTIGQEHWLADLVDPDAPLVQWRPSNERRREQR
                     SRSSD"
     gene            complement(669..1094)
                     /locus_tag="VFV93_12505"
     CDS             complement(669..1094)
                     /locus_tag="VFV93_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166213.1"
                     /translation="MVVWVALIAASAMTLLTLRSMAYAVANSVDARPRPSAARQSGTR
                     RIRRSYLSSHEAALLMIEIALSVGIALTTIGLASMAALPGRMLVGALLAQLAVLGSLI
                     APAVTVKLGGIGVRPAAVALGVAQLANALFFVRAICALI"
     gene            1352..2098
                     /locus_tag="VFV93_12510"
     CDS             1352..2098
                     /locus_tag="VFV93_12510"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX5166214.1"
                     /translation="MKHEEQAMALADDIANWIGEKVRGAGVNGAVMGLSGGIDSAVVS
                     GLCAKALGPKNVLGVIMPIHSNPEDTAYGVETAESWGIEQRTIDLSGAYDALVQILPP
                     GSDLANANIKPRLRMITLYHLANTMGRMVIGTGNRSELMAGYYTKYGDGGTDLLPIAG
                     LYKWQVRELARAIGVPQAIVDKPPSAGLWVGQTDEQEMGISYADLDRTLEAIDAGQES
                     TIEPDLLDKVTSMIRASEHKRHLPPIYGRE"
BASE COUNT          385 a          688 c          706 g          321 t
ORIGIN      
        1 gtcggtgggc cgctgctgac cgattgttac gcgatcatcg ttcccaacca gggcgccgtc
       61 atgatcgacg cgccccgcga cgcctggcag gccgccatcg aggccgccga ggcccagaac
      121 gcgcggacgc tgctggcgat cgcgacccac ggccactggg accacatcac cgatatggcg
      181 cgcgttcgcg agcagggcat tccggtcgcg gcgaacctgg ccgacgccaa cctgttcgcc
      241 gacccgcacg gtcaggggtt gacgctgccg ttcgcggttg atcccgtcac gatcgaccgg
      301 cccctgaacg agggtgatcg gctggcaatc ttcaattacg agctgcacgt cctgcacacg
      361 ccggggcaca ctcagggaag cgtcacgatc tgggtgccgg cgattgacgc gctgtttacg
      421 ggagatacgt tgctgaaggg cggcgccggc cacacggccg aaccgggcgc gagtcttcct
      481 gccctggcga ccagcgtccg gcgcctggcg gcgttcccgg agtcaacgac gctctatccg
      541 gggcatggcg cgccgaccac gatcgggcag gagcactggc tggcagacct ggtcgatccc
      601 gacgcgccgc tggttcagtg gcggccaagc aacgaacgcc gccgggagca gcgatcgcgg
      661 tcctcggact agatgagcgc gcagatggct cgaacgaaga agagcgcgtt ggcgagttgc
      721 gcaacaccga gcgccacggc tgccggtcgg acgccgatgc caccgagctt gaccgtcacg
      781 gccggagcga tcaacgagcc gaggacggcc agttgcgcca gcagcgcacc gacgagcatc
      841 cggcccggga gcgccgccat cgacgcgaga ccgatcgtcg tcaatgcgat accgaccgag
      901 agagcgatct cgatcatcag cagcgccgcc tcgtgcgagg acaagtagct gcggcggata
      961 cgtcgcgttc ctgattgtct cgctgctgac ggtcgtggac gggcatcgac gctgttcgcc
     1021 accgcgtatg ccatcgacct caatgtcagc aacgtcattg cgctcgccgc gatgagcgcc
     1081 acccagacga ccacggcttc ctccagtacc ccgcgccacc ccatggcggc acgtaccatc
     1141 ccatcaacgg cccgcgacgt gcgatacgtc gcttccgtca tccctgtcgc atgtagaacg
     1201 aacgagcgag tgaatggatg cgtgaattga tcaggaaatt tgtgcgcggt cgctgcgacg
     1261 cctgactgtc tcatccggcg cttgactgat cgccgccggc tggtaccgtg atagcgagca
     1321 gggcggccct gagacgccgc agctggcgac gatgaaacac gaggagcagg cgatggcgct
     1381 ggcggatgac atcgcgaact ggattggcga gaaggtacgc ggcgccggag tgaatggcgc
     1441 cgtgatgggg ctgagtggcg ggatcgactc ggccgtcgtc tcgggcctct gcgcgaaggc
     1501 actcggcccg aagaacgtcc tcggcgtcat catgccgatc cactcgaatc ctgaggacac
     1561 cgcctacggc gtcgagacgg ccgagtcctg gggtatcgag cagcgcacga tcgacctgtc
     1621 gggcgcctac gacgcgctgg tgcagattct gccgccgggg agtgatctgg ccaacgcgaa
     1681 cattaagccc cggctgcgca tgatcacgct ctatcacctg gccaacacga tgggccggat
     1741 ggtgatcggc accgggaacc gcagcgagct gatggccggc tactacacca agtacggtga
     1801 cggcgggacc gatttgctgc cgatcgccgg tctctacaag tggcaggtgc gcgagctggc
     1861 gcgtgcgatc ggcgtgccgc aggcgatcgt cgacaagcca cccagcgccg gtctctgggt
     1921 tggccagacc gacgagcagg agatgggcat cagctacgcc gatctcgatc ggacgctgga
     1981 agcgatcgat gccggccagg agtcgacaat tgagccggat ctgcttgaca aagtcacctc
     2041 gatgatccgc gcctcggagc ataagcgcca cctgccgccg atctacgggc gcgagtagcg
//
LOCUS       DASUGJ010000551         4586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1617980, whole genome shotgun sequence.
ACCESSION   DASUGJ010000551 DASUGJ010000000
VERSION     DASUGJ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617980"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VFV93_12515"
     CDS             <1..1338
                     /locus_tag="VFV93_12515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5166215.1"
                     /translation="DNRNNSLSDIWVTDLHNNESRRVTPEDGNYSTPAWSPSGELAYV
                     GHPIVEPYGPSTLDNLYTWSPDSDEINGLLVGIDREPGNGAIADARYGVPTQLPIWSS
                     DGSQVYTLLSDRGSVDMYTCPTSGSPSSLAGGQRDIQSFTVSNDGTIAFAVSTMQLPT
                     EVFVRRSDGSETQLSHHNDDFINGIELGSIEEVTFESDPGVEIHGWLLRPPAFNADAK
                     YPAVLEIHGGPHGMYGVGFFHEMHVLAARGYVVLYTNPRGSTGYGQNFVAASMNDWGG
                     ADYRDVMAGVPYLESLGYVDMTRLGVTGGSYGGYMTNWIIGQTNQFKAAVTMRSTCNR
                     ISQYGTSDFNMMYGDWEFKGTPYDSPEFYLERSPLTYVKNVTTPVLILHSENDLRCPI
                     SQGEELFVALKKLGKEVEFVRFPDESHGLSRAGQPVHRIERLERLTGWFDSHL"
     gene            1516..1902
                     /locus_tag="VFV93_12520"
     CDS             1516..1902
                     /locus_tag="VFV93_12520"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX5166216.1"
                     /translation="MRKRWLTLLSVAIVAMLVLAACGSGDDDDDDAAADLSGTPVSMK
                     DSMKFEPDTITVKAGSEVEIALSNDGAIKHNFSIDDADVDEDLDGGKSKSISFTAPDS
                     PGEYKIHCAIPGHESAGMVATLIVEE"
     gene            2079..3506
                     /locus_tag="VFV93_12525"
     CDS             2079..3506
                     /locus_tag="VFV93_12525"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX5166217.1"
                     /translation="MPMRAYESRNELLDAFGKYGSADVKARTVSIRDATGLRRDLIDN
                     LAYTAVFGADEPRAVARWLIWETAQQLGCRPASIHDFYIAGGRGMWNNQTTPAINVRA
                     LAYDTARTIFRSAQKLDVGQVIFEIARSEMGYTNQRPEEYASSVLAAAIREGHQGPVF
                     IQGDHFQINAKGYKADGEKEVQGVRDLIDEALAAGFYNIDVDTSTIVDLSLDGEDAQQ
                     ADNAFRSAQLTAYIREHQPKDITVSVGAEIGEVGTENSTVAELHAFMRAYQRELAGMN
                     GGSLTGISKISVQTGTSHGGIVLPDGSIAKVKVDFDTLGELSSVARSDYHLGGAVQHG
                     ASTLPEEAFDRFAQANACEVHLATAFQNIVYDSEAFPDDLRNEIYAYLEEHHQNERKP
                     DMTDAQFYYTARKRGIGPFKQQMWDLPDDARGQIMSELGSRFELIFSRLGVTNTMSMI
                     RDITPTPSLPYRQGKAISDVEVEGE"
     gene            complement(3478..3762)
                     /locus_tag="VFV93_12530"
     CDS             complement(3478..3762)
                     /locus_tag="VFV93_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166218.1"
                     /translation="MQRGFAGTTDDHNQYFTCNECGRVTFEILSRTQREIRVGRLEPG
                     RQVRHEGGEYVVSRVLKVGLNESLVYLKPADDAGSGVPRGSATRLPPRRR"
     gene            complement(4127..>4586)
                     /locus_tag="VFV93_12535"
     CDS             complement(4127..>4586)
                     /locus_tag="VFV93_12535"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX5166219.1"
                     /translation="GRESSASPRPASRVPRPADDTERGSIMMIVATDAPLLDRGLRRL
                     ARRAGIGLARTGSIAGNGSGDYVIAFSASELVRVPHEPKGLTHRFEVVVEDGPAIDGL
                     FQATVEATEEAILNALFRATTLTGRDGNTREALPLGAVREIMRAHGRLFS"
BASE COUNT          907 a         1651 c         1285 g          743 t
ORIGIN      
        1 gacaaccgca acaactctct cagcgatatc tgggtcaccg acctgcacaa caacgagtcg
       61 cgccgcgtca cccccgagga cggcaactac agcactccag cctggtcgcc ttccggcgag
      121 cttgcctacg tcggccaccc gatcgtcgag ccatacggcc catctacgct cgacaacctc
      181 tacacctgga gtccggacag cgacgagatc aacggcctgc tggtcggcat cgaccgcgag
      241 ccgggtaacg gcgccatcgc cgacgcccgc tacggcgtcc cgactcagct tccaatctgg
      301 tccagcgacg gctcacaggt ctatacgctg ctctccgatc gtggcagcgt cgatatgtat
      361 acctgcccca ccagcggcag cccgtcatca ctcgccggtg gccagcgcga catccagtcc
      421 ttcacggtct ccaacgacgg cacaatcgcc ttcgccgtca gtacgatgca gctcccgacc
      481 gaagtcttcg ttcgccgatc ggacggtagc gagacccaac tctcccacca caacgacgac
      541 ttcatcaacg gcatcgaact gggcagcatc gaagaggtca ccttcgagag cgaccctggc
      601 gtcgagattc acggctggct cctgcgcccg cccgccttca acgccgacgc gaagtatccg
      661 gccgtgctcg agatccacgg cgggccgcat ggcatgtacg gtgttggttt cttccacgag
      721 atgcacgtcc tcgccgccag gggctacgtc gtcctctaca ccaacccgcg tggcagcacc
      781 ggctacggcc agaacttcgt cgccgcctcg atgaacgact ggggcggcgc cgactaccgc
      841 gatgtcatgg ccggagtgcc gtacctcgaa tcgctcggtt atgtcgatat gacccgtctc
      901 ggcgtcaccg gcggctccta cggcggctac atgaccaact ggatcatcgg ccagaccaac
      961 cagttcaagg ctgccgtcac gatgcgctcg acctgcaacc gcatctcgca atacggcacc
     1021 agcgacttca acatgatgta cggagattgg gaattcaagg gcacccccta cgatagcccc
     1081 gagttctacc tggaacgctc gccactcacc tacgtcaaga atgtgacgac gccggtcctc
     1141 atcctgcatt ccgagaacga tctgcgttgc ccgatcagcc agggcgagga gctcttcgtt
     1201 gccctcaaga agctcggcaa ggaggtcgag ttcgtccgct tccccgacga atcccacggc
     1261 ctctcccgcg ccggccaacc cgtccaccgt atagaacgcc tcgaacgcct gacaggctgg
     1321 ttcgattcgc atctgtaatg tatcgggatt gggggtaggc aaccgcgcgt gttccgaggg
     1381 acgatggtgg tcgactagcc ccgaaccccg agtcccgaat cccgacaccg ccgtatactc
     1441 atcttcgcgg ccgaagcgac cgcgattcga tggccgctcc cggcggccgg caccagaccc
     1501 ggaaggggag atttcatgcg aaagcgatgg ctgacgctgt tgtcagtcgc gattgtcgcg
     1561 atgcttgttc tggccgcctg cggcagcggc gacgatgacg acgatgatgc cgccgccgac
     1621 ctcagtggca caccagtctc gatgaaggac tcgatgaagt tcgaacccga caccatcacc
     1681 gtcaaggccg gtagcgaggt cgagatcgcc ctcagcaacg acggcgccat caagcacaac
     1741 ttcagcatcg acgacgccga tgtcgacgag gacctcgacg gcggcaagtc caagtcgatc
     1801 tccttcaccg cccccgactc tcccggcgag tacaagatcc actgcgccat ccccggccac
     1861 gagtcggccg gcatggtcgc gaccctcatc gtcgaggagt aagcgtcggg actcgggatt
     1921 cgggaatcgg ggctggacga ccaccatcgt ccctcgcaac acccgcggtt gtttacccct
     1981 gaatcccgag tcccgaatcc cgccccccgc gtcccgaagt atcattaccc catggccgct
     2041 gcccggcggc acgctcagca gacagggagg ggatcaggat gccaatgcgc gcgtacgaat
     2101 ccaggaacga gttgcttgat gccttcggca agtacggatc ggccgatgtc aaggcacgaa
     2161 ccgtctcaat ccgtgacgca accggtctcc gtcgcgacct gatcgacaac ctggcctaca
     2221 cggccgtctt cggcgccgac gaaccgcgcg ccgtcgcccg ctggctgatt tgggagacag
     2281 cccagcaact cggctgccgc ccggcatcga tccacgactt ctacatcgcc ggtggccggg
     2341 gcatgtggaa caaccagacg acgccggcca tcaacgtccg cgccctggcc tacgacaccg
     2401 cccggaccat cttccgctcg gcccagaagc tggacgtcgg ccaggtcatc ttcgagattg
     2461 cccgctccga aatgggctac accaaccagc gcccggagga gtacgcctcc tcggtcctgg
     2521 ctgccgccat ccgtgagggc caccagggac cggtcttcat ccagggcgac cacttccaga
     2581 tcaacgccaa gggctacaag gccgacggcg agaaggaggt ccagggcgtt cgcgacctga
     2641 tcgacgaagc cctcgcggcc ggcttctaca acatcgatgt cgacacgtcg accatcgtcg
     2701 acctctcact cgatggtgag gacgcccagc aggccgacaa cgccttccgc tcagctcagc
     2761 tgaccgctta catccgcgag caccagccga aggacatcac cgtctcggtc ggcgccgaga
     2821 tcggcgaggt cggcaccgag aattcgactg tcgccgagtt gcacgccttc atgcgtgcct
     2881 atcagcgcga actggccggc atgaacggcg gtagcctgac cggcatctcc aagatcagcg
     2941 tccagacagg caccagccac ggcgggatcg tcttgcccga cggctcgatc gccaaggtca
     3001 aggtcgactt cgacacgctc ggtgagctct ccagtgtggc ccgctccgac taccacttgg
     3061 gcggcgccgt ccagcacggc gcgtcgaccc tgccggagga agccttcgac cgcttcgccc
     3121 aggccaacgc ctgtgaagtc cacctggcaa ccgcatttca gaacatagtc tacgacagcg
     3181 aagcgttccc cgacgatctg cgcaacgaga tctacgccta cctggaggaa catcaccaga
     3241 acgagcgtaa gcccgacatg accgacgccc agttctatta cacagcccgc aagcgcggca
     3301 tcggaccatt caagcagcag atgtgggacc tgcccgacga cgcgcgcggc cagatcatgt
     3361 ccgagctggg gagccgcttc gagctgatct tctcacggct cggcgtcacc aacacgatga
     3421 gcatgatccg cgacatcacg ccgacgccca gcctgcccta ccggcagggc aaggcgatca
     3481 gcgacgtcga ggtggaaggc gagtagccga cccgcgcggc acgccggaac cggcatcgtc
     3541 ggccggcttg agatagacca gggattcgtt cagtccgacc ttgaggaccc gcgagacgac
     3601 gtactcgccg ccctcatggc gcacctgacg ccccggttca agccggccga cccggatctc
     3661 ccgctgcgtt cgtgacagaa tctcgaacgt cacccggcca cattcgttac aggtgaaata
     3721 ctggttgtga tcgtcggtcg tcccggcaaa gccgcgctgc accagcgccg tatcctcact
     3781 cccacactcc gggcacgtcc gttgctgaac ttgctcgctc gccaatcgct cgcccaatcg
     3841 taaaggagcc acccggccac ccgttgctgc gttgcgtacc gtgattctaa ccaagatcgc
     3901 gtctaactcc gcaagcccca ccggccacat acgtgagact cggcccaatc gaacggcgtt
     3961 gcagaggaac aatcccgctt cagcctgtca tcctgacgca gaaggaacct cgtcactcat
     4021 cgacgcggaa cggactgtac gagaacgaga tccttcgctg ccgctcagga tgacagggga
     4081 agcgcggcta ttccgcccca acgcatcacg cgtcacgcat cacacatcac gaaaacagcc
     4141 gtccatgtgc ccgcatgatc tcgcgaactg cacccagcgg caatgcctct cgcgtgttgc
     4201 catcccgacc ggtcagcgtc gtcgcccgga acagcgcatt caggatcgcc tcttcggtgg
     4261 cctcgaccgt tgcctgaaac aatccatcaa tcgccggccc atcctcgacg acaacctcga
     4321 accgatgcgt cagccctttc ggctcgtgcg gcacccggac cagttccgac gccgagaacg
     4381 cgatcacgta gtcgccactc ccgttcccgg cgatcgaccc cgtccgcgcc agcccgattc
     4441 ccgcccgccg cgccagccgc cgcaaccccc gatccagcaa cggcgcatcc gtcgcgacga
     4501 tcatcatgat tgagccacgt tcggtatcgt cggcgggacg cgggacgcgg gacgcgggac
     4561 gcggcgatgc gctcgattcg cgtccc
//
LOCUS       DASUGJ010000552         4773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_922678, whole genome shotgun sequence.
ACCESSION   DASUGJ010000552 DASUGJ010000000
VERSION     DASUGJ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922678"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFV93_12540"
     CDS             <1..549
                     /locus_tag="VFV93_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5166220.1"
                     /translation="VYALPYPDAAFDAVNANSLLQHLSDPVRALREFRRVLRPGGIVA
                     VRDPDWSTMILEPTTPLIELLRQLRIRIYAHHGSDATCARHQRRLLLDAGFDYVECYP
                     TAQPASGGTPAHVREHARAMRDYLGVSADLIVAERMADAVTVEALDAELLAWSERPDA
                     TVALLFFCAIGWVNAPSISDDW"
     gene            570..1688
                     /locus_tag="VFV93_12545"
     CDS             570..1688
                     /locus_tag="VFV93_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5166221.1"
                     /translation="MADRLRVAVLGAGRWAQTAHIPGWQRDPRCQIVVICDPVEDRAR
                     DFAHEFGIPEHSRDWQAVVGRDDIDIVDVATPSRSHFELAWAALESGKHVLCEKPVAY
                     DFRQTRSAAELARSKGLKTKLGFTFRYSPGIQYAWSLIQEGFVGTPFIFNGYEQNSQW
                     LDPQTPLRQGVPDPPNPPRIATRSLEGYGAPIIDIGHWWVGADYSRVIGTMRNFIPER
                     MVRDTGLMTRVNIDDGTIYLHEYTNGAIGSIQTSLVTIGNYPGVEARLYGERGAIICR
                     LVEEFGVAETIKVAASDDVEFRELEIPERFYPAGGHPRELWRTLFYSNLIRDFLDEIT
                     DGADRNQGNFDDGAWVQEAINAVETSFHERRWVDLPLA"
     gene            1898..3517
                     /locus_tag="VFV93_12550"
     CDS             1898..3517
                     /locus_tag="VFV93_12550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166222.1"
                     /translation="MTSVDASVLSQGEPLSPASAAHNRRLSILLSMAMFVLVVDTSLM
                     NVSISAVSRDLNMTVSGVQSAIALEALVSAAFILISSKLGDLFGRKRAYVLGLLGYAA
                     GAGAMMFAQSQLAIIIFWAIIGGLGASLLLPAMQSLVHGNFDGEERRKAYAQVGASAA
                     IATAVGPLLGGFITTYLSWRVGFLLELILIAVVLSGLGLVKDVPYTGSRKLDPIGSLL
                     SVVGMGGIVLGILVWQEGGESVAALLAVGAFALGSLVWWLVRRKRQEKPTLLDPGLFE
                     SKYFRLGITQQTFQQIALGGMMIALPIYLQMVLEYNALGAGLSLAPLSLSMFAVALLA
                     GKRAGNRRPANIVLVGFVLLTVAVGTLLPIVPRADSGWDLAISLVVAGCGLGLLVSQL
                     NNYTLSPVSEERASEAAGVNSAGGSFGLSLGLALSGALMLATLAFSFTDMAEASDVLA
                     PEQQQQVATALEEDAEIMTNTQLQEQLAGQTEEVQNEIIRINNETRDLSLQVALLVPL
                     VVGLLGIVTALRMRRLPDPEPSEAAESLLVG"
     gene            3541..>4773
                     /locus_tag="VFV93_12555"
     CDS             3541..>4773
                     /locus_tag="VFV93_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HEX5166223.1"
                     /translation="MVDRGPQVFISYQRHDGEFARHVRGYLVAAGVQTWMDQFDIPVG
                     AYWPDEIDKGLASSDIVVGVISPEAVESRNVKNEWDWALQNDKPLLLLQYRPCVIPHR
                     YVSINFIDATAADPTNSLDALLRTLGHGPAGGASMAAEASAPPPPIYHARRSVVRRTR
                     PEPTLVGRDQEQATLRMVLDDVLAGHGSLVLVGGEAGAGKTILTNWIAWLAEERGALT
                     LSGGCYDLSTPRPYQPWAEIIWNWPDDLPPLPEDLRSDDGFATIRSQAALVELLATCL
                     ADASARRPLVLQLEDLQWADQASLDLLRALTRLIDGQRILLVVTYRDDELTRRHPLYG
                     LLPGLAREQNAQRINLTRLPLDETRRLVSASYDLAEDDLSRVAIYVHNLAEGNPFFSG
                     EVLRALASSGALVYDRDGW"
BASE COUNT          756 a         1506 c         1603 g          908 t
ORIGIN      
        1 gtctacgcgt tgccctatcc cgacgcggca ttcgatgcgg ttaatgccaa ctcgctgcta
       61 cagcacttga gcgaccctgt ccgggcgctg cgcgagttcc ggcgcgtgct gcggccaggc
      121 ggcatcgtgg ccgtgcggga ccccgattgg agcacgatga tcctggagcc cacgaccccg
      181 cttatcgagc tcttgcgcca gttgcgaatc cgcatctacg ctcaccacgg cagcgacgca
      241 acgtgcgcac ggcatcagcg tcgtctcctg cttgatgcgg gcttcgatta tgtcgaatgc
      301 tacccgacgg cgcagcctgc ttcgggcggt acaccggctc atgtgcgtga gcatgcacgc
      361 gcgatgcgag actatctggg ggtctctgcc gatctcatcg tggcggagag gatggcggat
      421 gccgttacgg tagaagcgct tgacgcggaa ctcctcgcgt ggagtgagcg gccggatgcg
      481 acggtggcgt tgctcttctt ctgtgcaatc ggctgggtca atgcgccaag tatttctgac
      541 gactggtagg tcgagggcgg gagggtctca tggctgacag attacgcgtc gcggtgcttg
      601 gcgccggtcg ctgggcgcag acggcgcaca tccccggctg gcagcgtgat ccccgctgcc
      661 agatcgtcgt catctgcgat ccggtcgagg atcgtgcccg tgacttcgcc cacgagttcg
      721 gcatccccga gcatagccgc gactggcagg cggtcgtcgg gcgggatgac atcgacatcg
      781 ttgacgtcgc aactccctcg cggtcgcact tcgagctggc ctgggcggcg ctggagtcgg
      841 gcaagcatgt cctctgcgag aagccggtcg cctacgactt ccgccagacg cggtccgcag
      901 ccgagttggc gcgcagcaag gggctcaaga cgaagctcgg cttcacgttt cgctatagcc
      961 ccggcatcca gtacgcctgg tcgctgattc aggagggctt cgtcgggacg ccgttcatct
     1021 tcaacggcta cgagcagaac tcgcagtggc ttgacccgca gacgccgctg cgccagggcg
     1081 tgcccgaccc gccgaatccg ccacgaatcg cgacccgttc gctggagggc tacggcgcgc
     1141 cgatcatcga tatcggccac tggtgggtcg gggcggatta cagccgcgtg atcggcacga
     1201 tgcgcaactt catccccgag cggatggtgc gcgacaccgg cctgatgacg cgtgtcaaca
     1261 tcgacgacgg cacgatctac ctgcacgagt acaccaacgg cgcaatcggc tcgatccaga
     1321 ccagtctggt gacgatcggc aactatccgg gcgtcgaagc ccgcctctac ggcgagcggg
     1381 gcgcgatcat ctgccgattg gtcgaggagt tcggtgtcgc cgagacgatc aaggtcgcgg
     1441 catccgatga cgtcgagttc cgcgaactgg agatccccga acgcttctac ccggccggcg
     1501 gccacccgcg cgaactgtgg cggacactct tctactccaa cctgatccgc gacttcctcg
     1561 acgagatcac cgacggcgcc gaccgcaacc aaggcaactt cgacgacggc gcctgggttc
     1621 aggaggccat caacgcggtc gagacgtcgt tccacgagcg ccgctgggtg gatctgccgt
     1681 tggcgtgatc cgcgattggc agtgcgctga cagcgggcgg ggctttggtc atcgcggcga
     1741 cgagggctgg ctgcctgcga cgatacaacc gtcaaataca ccgtgttggg gcttcagcgg
     1801 ctctccccgt tcatccacga tatcgctggc acactcgcgg gtgtgccgga cgagttgtgc
     1861 gttcggacag caactgctga cgactctgga gacccctatg acgagtgtgg acgccagcgt
     1921 cctgagccag ggtgagcctc tttcgcctgc atcggcggct cataatcgcc gtctctcgat
     1981 cctgctgtcg atggcgatgt tcgtgctcgt tgtcgataca tcgttgatga atgtctcgat
     2041 ctcagccgtc agtcgcgatc tgaacatgac cgtcagtggt gtccagtcgg cgatcgcgct
     2101 tgaagcgctc gtctcggctg ccttcatcct gatcagcagc aagctcggcg acctgtttgg
     2161 gcgcaagcgg gcgtatgtgc tggggttgct cggctatgcg gccggcgccg gcgcgatgat
     2221 gttcgcgcag agccaactcg cgatcatcat cttctgggcg attatcggcg ggcttggtgc
     2281 ctcgctgctg ttgccggcga tgcagtcgct cgtgcacggc aacttcgatg gcgaggagcg
     2341 caggaaggcg tacgcccagg ttggcgcgtc ggcagcgatt gcgactgcgg ttgggccgct
     2401 gctcggtggg ttcatcacca cctatctctc ctggcgtgtg ggattcctgc ttgaactgat
     2461 cctgattgcg gtcgtgctct cgggtctcgg gttggtgaag gatgtgccct acaccggatc
     2521 gcgcaagctc gatccgatcg gttcgctcct ctcggtcgtc gggatgggcg ggatcgtcct
     2581 gggtatcctg gtctggcagg agggtggcga atcggtcgcc gcgctgctgg cggtcggagc
     2641 gtttgcgctg gggtcgctcg tctggtggct cgtcaggcgc aagcgccagg agaagccgac
     2701 gttgctcgac ccggggctgt tcgagtcgaa atacttccgg cttggcatca cccagcagac
     2761 gttccagcag atcgcccttg gcggcatgat gatcgcgctg ccgatctacc tgcagatggt
     2821 gctggagtac aacgcgttgg gggccgggct gtcgctcgcg ccgctgtcgc tcagcatgtt
     2881 cgcggtggcg ctgctggccg ggaagcgggc cgggaaccgt cgccccgcca acatcgtcct
     2941 tgtcgggttt gtgctgctga ccgtcgcggt cggcacgttg ttgccaatcg ttcccagggc
     3001 agattccggc tgggatctgg cgatttcgct ggttgtcgcc ggctgcgggc tggggctgct
     3061 ggtctcccaa ctcaacaatt acacgctatc gccggtgtcc gaggagcgcg ccagcgaggc
     3121 ggccggcgtc aattccgcgg gcggctcgtt cgggctgtcg ctggggctgg cgttgtccgg
     3181 cgcgctgatg ctcgcgacac tcgccttttc gttcaccgat atggcggagg ccagcgatgt
     3241 gctcgcgccc gaacagcagc agcaggtcgc gaccgcgctt gaggaagacg cagagatcat
     3301 gaccaacacc cagctccagg agcaactggc cggtcagacc gaggaggtcc agaacgagat
     3361 catccgcatc aacaatgaga cgcgggatct ctcgctccag gtggcgctgc tcgttccgct
     3421 cgtggttggg ctcctcggca tcgttaccgc gttgcggatg cggcgactcc cggaccccga
     3481 accatccgaa gccgccgaat cgctgctcgt gggctgatgt cgagcggaag cagggcgggc
     3541 atggtcgatc gcggccctca ggtcttcatc tcctaccagc gacatgacgg cgagttcgcg
     3601 cgccacgttc gtgggtacct ggttgcagcc ggcgtgcaga cctggatgga ccaattcgac
     3661 attccggtcg gcgcttactg gccagatgag attgacaagg ggctggcatc gagcgatatc
     3721 gtcgtcggag tgatctcgcc ggaggccgtc gagagtcgca atgtcaagaa cgagtgggac
     3781 tgggcgctgc agaacgacaa accgctgctc ttgctccagt accggccatg tgtcattcca
     3841 caccgctacg tctcgatcaa cttcatcgat gcgaccgcgg cggacccgac gaactcgctg
     3901 gacgcgttgc tgcggacact cggacacgga ccggctggcg gtgccagcat ggcagccgag
     3961 gccagcgcgc cccctccgcc gatctatcac gctcgacgga gcgttgtccg gcgaacgcgc
     4021 cccgaaccga ccctcgttgg ccgtgaccag gagcaggcaa cgctccggat ggtgctggac
     4081 gatgtcctcg ccggacacgg gtcgctggtg ctggtcggtg gcgaggccgg cgcaggcaag
     4141 acgattctga cgaactggat cgcctggctt gccgaggagc ggggtgcgct gacgctcagc
     4201 ggcggctgct acgacctctc gacgccgcga ccgtaccagc cgtgggctga gatcatctgg
     4261 aactggccgg acgacctccc gccactcccg gaggatctgc gcagtgacga cgggttcgcg
     4321 acgattcggt cgcaggcggc gttggtcgaa ctgctcgcga catgcctggc cgacgcgagc
     4381 gcacgccggc cgctcgtcct ccagttggag gatctgcaat gggccgacca ggccagtctc
     4441 gatctgttgc gggcgctgac acgactgatc gacgggcagc gtattctgct cgttgtcacg
     4501 taccgggacg acgagctgac ccgccgccac ccgctctatg gcctcctgcc gggcttggcg
     4561 cgcgagcaga acgcgcagcg tatcaacctg acgcgactac cacttgacga gacgcgccgg
     4621 ctggtctccg cgagctatga cctggcggag gatgatctca gccgggtggc gatctacgtc
     4681 cacaatctcg cggagggcaa cccgttcttc agtggcgagg tgcttcgcgc gcttgcgtcc
     4741 tcgggagcgc tcgtgtatga ccgcgacggt tgg
//
LOCUS       DASUGJ010000553         4661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1894441, whole genome shotgun sequence.
ACCESSION   DASUGJ010000553 DASUGJ010000000
VERSION     DASUGJ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894441"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFV93_12560"
     CDS             complement(<1..313)
                     /locus_tag="VFV93_12560"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5166224.1"
                     /translation="MSGKRSATEAQQELERLVAGVDGSVGVAAWPVGKPDQAIGVNMD
                     ELYPTASTFKIPLIYALYQMTDREEIALTKRVTIEESDRVPGSGVLQDLDPGLSPTIR
                     DL"
     gene            799..2112
                     /locus_tag="VFV93_12565"
     CDS             799..2112
                     /locus_tag="VFV93_12565"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166225.1"
                     /translation="MRLKRGGLLANHDFLQLWASESVSQVGAQVTVVALPLLAALSLD
                     ANAAEMGLLTAAGTAPPLLLGLVAGVWVDRLRRRPIMVATDLGRAAVLLLIPILWWLG
                     LLRMELLYLITFVAGVQTLFFDVAWVTFLPSVVRRDQLVEGNSKLQATASGAQVAGPG
                     LAGLLVGLAGAPVAVLVDAVSYLLSAFFILRVRTPEPRPERAADAHVLREIREGLGVV
                     FRNPLLRAIAASGATVSLFGFMFLAVYVLFMTEELGFSATSVGLVFSLGGLGAVIGSM
                     LAEPLKRRIGIGPAIIFGRIGFGLGGLLVPLAVLAPSFMVPLVLGAEFLQWLMYLIAT
                     INEVSLRQSITPHRLLGRVNATMRFLNSGMIPIGALAGGALGGLIGLRETLFVGVFGM
                     LLTGIWVSLSPLRTTREEPSLLDEHGELLDTRTEEPTSLVVDASR"
     gene            complement(2102..3082)
                     /locus_tag="VFV93_12570"
     CDS             complement(2102..3082)
                     /locus_tag="VFV93_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5166226.1"
                     /translation="MAAIASRMGWISVVVGLLVLLAGCGESDDSGQAGDQAPPQESAT
                     AGAGSEHQHMGGSPVAGEPGDAMQVFLVASQLVVGQNRFAVGLTTPDNALIDDADVTF
                     EYFDLSNPAAPVSESKAEATRLSSTDGLTVIYAQERSFDRAGAWGVLIDATRPDGTTL
                     SRNISFDVLADSPTLAIGEKAPAIDSPTSADVDGDLTKLTSASKPNPAFYEMTIADAL
                     ADGKPTVVLFATPSYCQTRFCGPDYDIVSDIQQRYGAQFNAIHVEVYSGLPNPAASGW
                     QYNQPLIDFGVQTEPWLFVINAAGVVTWRVEGLFTEDEVVAALRDVGVTG"
     gene            complement(3440..>4661)
                     /locus_tag="VFV93_12575"
     CDS             complement(3440..>4661)
                     /locus_tag="VFV93_12575"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX5166227.1"
                     /translation="PHPASRVTTSERSTPTMRALTWHGKNDVRVENVPDPLILNPRDA
                     IVRITSTAICGSDLHLYDGFIPTLEPGDILGHEFMGEIVETGSGVSNLKVGDRVVVPF
                     TIACGNCFYCKSQLWSLCDNSNPNADMAEKMYGFSGSGLYGYSHLYGGYAGGQAEFAR
                     VPFADVGPIKVPDGMTDEQVLFLSDIFPTGYMAAENGDIKPGDTVAVWGCGPVGQFAI
                     QSAWMLGAEQVIAIDRVPERIRMAAVDGRATTINYEEVGDVVEALKELTGGRGPDVCI
                     DAVGMESHADGLDWWMDRAMQVAKVGFDRAHVLREVIQACRKGGVVSIPGVYAGVVQN
                     VPMGMAFAKGLTFKMGQTHMHRYLQPLLERVQAGDIDPTFVITHTLPLDDAPHGYRIF
                     RDKLDDCIKIVLKP"
BASE COUNT          716 a         1490 c         1602 g          853 t
ORIGIN      
        1 ccaggtcgcg gatcgtcggc gagagaccgg gatcgagatc ctggaggacc cccgagccag
       61 gcacgcggtc gctctcctcg atcgtcacac gcttggtgag cgcgatctcc tcgcggtcgg
      121 tcatctggta cagcgcatag atcagcggga tcttgaaggt gctggccgtt ggatagagct
      181 cgtccatgtt gacgccgatc gcctgatccg gcttcccgac cggccaggcc gcgacaccaa
      241 cgctgccatc gacaccggcc accagccgtt caagctcctg ctgcgcctcg gtcgcgcttc
      301 gctttcccga catcctgtcc ccttcctgtc cctcgtggat cgttcacgca cgcctccata
      361 gaataccgcc atcccatccg ggtgtgtcgc gcgacggcta cctgtccggt tggacagggc
      421 gtcgatgtcc actgtgcggt agcggaatgc gtggttaaca gcgtacgata gcgccctgca
      481 cggcgggcga ccgccgccga aggagcatcg tcgatgggat atcacggacg accggtgctg
      541 acggcctggg ccgaccggta cgacgaactg agcgtgagga tgcaagaggc cccgctgtcc
      601 tgagaatcgc gcaggtggac agcgggagcg ggcgcaaccg atcgcccctc ccgattcaac
      661 tcgggagtca ttgcacatgc ttgggaaggt atctcgtcac gcagactggg gcgacgccac
      721 tggtgctcgc cgcactccgt cagattcaga tctcaatcga gacgccgcac gcgcctggac
      781 ccgccaggag gtgtcgccat gcgcttgaaa cgcggcggcc tgctggccaa tcacgacttc
      841 ctgcaactct gggcgtccga atcggtctcg caggtcgggg cacaggtcac ggtcgtcgcg
      901 ctgccgttgc tggcggcgct ctcgctggat gcgaacgccg ccgagatggg cctgctgacg
      961 gcggccggga cggccccgcc gctgctgctg ggcctggtcg ccggcgtctg ggtcgaccgg
     1021 ctgcggcgac ggccgatcat ggtcgcgacc gacctcggcc gggcggctgt cctgctgctg
     1081 atcccgatcc tctggtggct gggactgctg cggatggagc tgctctacct gatcacgttc
     1141 gttgcgggcg tccagacgct gttcttcgac gtcgcctggg tgacgtttct gccctcggtc
     1201 gtccggcgcg accagctcgt cgagggcaac agcaagctcc aggcgaccgc ctccggcgcg
     1261 caggtggccg ggccgggcct ggccggcctg ctggtcggcc tggccggcgc gccggttgcc
     1321 gtcctggtcg acgccgtctc gtatctgctg tcggcgttct tcatcttgcg ggtacggaca
     1381 ccggaaccgc ggccggaacg ggccgccgac gcgcacgtcc tgcgtgagat ccgcgaggga
     1441 ctgggggtcg tgttccgcaa cccgctgctg cgggcgatcg ccgcttcggg cgcgaccgtc
     1501 agcctgttcg gcttcatgtt cctggccgtc tatgtcctgt tcatgaccga ggagcttggc
     1561 ttctcggcga catcggtcgg gctggtcttc agtcttggtg ggctgggggc ggtcatcggg
     1621 tcgatgctgg ccgagccgct caagcgcagg atcgggatcg gcccggccat catcttcggc
     1681 cggatcggct tcgggctggg cgggctgctg gtgccgttgg cagtcctcgc gccgtcgttc
     1741 atggtgccgc tggtgctcgg cgccgagttc ctgcaatggc tgatgtacct gatcgcgacc
     1801 atcaacgagg tcagcttgcg ccagagcatc actccccacc ggctgctcgg cagggtcaac
     1861 gcgacgatgc gttttctcaa ctccgggatg attccgatcg gggcgctggc cggcggcgcg
     1921 ctgggcggcc tgatcggcct gcgcgaaaca ctcttcgtcg gcgtgttcgg gatgctgctg
     1981 accggcatct gggtctccct ctcgccgcta cggaccacgc gtgaggaacc gtcgctgctc
     2041 gacgagcacg gcgaattgtt ggacacgcga actgaggaac caacttcgct cgtggtcgac
     2101 gctagccggt aaccccgaca tcccggaggg ccgcgacgac ttcgtcttcg gtgaagaggc
     2161 cctcgacacg ccaggtgacg acgccggcgg cgttgatgac gaatagccac ggctcggtct
     2221 ggacgccgaa gtcgatcagc ggctgattgt attgccagcc gctggcggcc gggttgggca
     2281 ggccactgta gacctcgacg tggatcgcgt tgaactgcgc gccgtagcgc tgctggatgt
     2341 cgctgacgat gtcgtagtcg gggccgcaga agcgcgtctg gcaatagctg ggtgtcgcga
     2401 agaggacgac ggtcggcttg ccatcagcca gcgcgtcggc gatcgtcatc tcgtagaagg
     2461 ccgggttcgg cttgctggcc gaggtgagtt tggtcaggtc gccatcgaca tcggcggacg
     2521 tcggtgagtc gatggccggc gccttctcgc cgatcgccag cgtcgggctg tcggccagca
     2581 catcgaacga gatgttgcgc gacagcgtcg tcccgtccgg tcgagtcgcg tcgatcagga
     2641 cgccccaggc accggcccgg tcgaaggacc gttcctgggc gtagatgacg gtcagcccat
     2701 ctgttgacga gagacgggtc gcctcggcct tggactcgct gacgggcgcg gccggattgc
     2761 tgaggtcgaa gtactcgaac gtcacgtcgg catcgtcgat cagggcgttg tctggcgtcg
     2821 tcaggccaac ggcgaagcgg ttctggccga caaccagctg cgaggccacc aggaagacct
     2881 gcatcgcgtc acccggctca ccggcgaccg gcgagccgcc catgtgctgg tgctcgctac
     2941 cggcgccggc ggtggcggat tcctggggtg gcgcttggtc acccgcctgc ccagagtcgt
     3001 cgctctcacc acagccggcc agcaggacaa gcagcccgac gacaacggat atccagccca
     3061 ttcgactcgc gatcgctgcc atcgctcccc tcgcccctga atcatcacca gcggttctac
     3121 tgtaacaggg acgagtgggg gctgggcgtt acgggggtgc tgcgtgctgc gtggaaagtg
     3181 agccggatgg acgtggttgg tgaggaacag cacccttagc cctgtcatcc tgagcggcag
     3241 cgaaggatcg cctggcccga gcgacgcggg agtttcggga gcgacacttc gttctaccga
     3301 tcgatgcgct cgtgcatctg acgtttggcg atccttcgct gcggctcagg atgacagagc
     3361 tggagggttg ggtcctcctg aacggcgttc gtctgggtca ctcagcaccc agcacgcagc
     3421 actttccact cgccactcat cacggcttca gcacgatctt gatgcagtcg tcgagcttgt
     3481 cgcggaagat gcggtagccg tggggggcgt cgtcgagcgg gagcgtgtgg gtgatgacga
     3541 aggttgggtc gatgtcgccg gcctggaccc gttcgagcag cggctggaga tagcggtgca
     3601 tgtgggtctg gcccatcttg aaggtcagcc ccttggcgaa cgccattccc attgggacgt
     3661 tctggacgac accggcgtag acgccaggga tcgagacgac gccacccttg cggcaggcct
     3721 ggatgacctc gcgcaggacg tgggcgcggt cgaagccgac cttggcgacc tgcatcgcgc
     3781 ggtccatcca ccagtcgagc ccgtcggcgt gggactccat cccgaccgcg tcgatgcaga
     3841 catccgggcc gcgcccgccg gtcagctcct tgagcgcttc gacgacatcg ccgacttcct
     3901 cgtagttgat cgtcgttgcc cggccatcga cggccgccat ccgaattcgc tcgggaacgc
     3961 gatcgatggc aatgacctgc tcggcgccca gcatccaggc cgactggatc gcgaactggc
     4021 cgaccggccc acagccccag acggcgacgg tgtcgcccgg cttgatgtcg ccattctcgg
     4081 ccgccatgta gccggtcggg aaaatgtcgg agaggaacag cacctgctcg tcggtcatgc
     4141 cgtcggggac cttgatcggg ccgacatcgg caaagggaac acgggcgaac tcggcctgtc
     4201 cgccggcgta gccgccgtac agatgcgagt agccgtagag gccggagccg gagaagccat
     4261 acatcttctc ggccatgtcg gcgttggggt tcgagttgtc gcagagcgac cagagctggc
     4321 tcttgcagta gaagcagttg ccacaggcga tcgtgaacgg gacgacgacg cggtcaccga
     4381 ccttgaggtt cgagacgccg ctgccggttt cgacgatctc acccatgaac tcgtggccga
     4441 ggatgtcgcc aggctccagc gtcgggatga agccgtcata caggtgcagg tcggacccac
     4501 agatcgccgt tgaggtgatt cggacgatcg cgtcgcgagg attcaggatc aacgggtcgg
     4561 ggacgttctc gacgcggaca tcattcttgc cgtgccaggt gagtgcgcgc atggtggggg
     4621 tgctccgttc gcttgtcgtg acacgtgacg cgggatgcgg g
//
LOCUS       DASUGJ010000554         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_366959, whole genome shotgun sequence.
ACCESSION   DASUGJ010000554 DASUGJ010000000
VERSION     DASUGJ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366959"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFV93_12580"
     CDS             <1..354
                     /locus_tag="VFV93_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166228.1"
                     /translation="DDGRIGYVRLREVEAAIGSDTTPTRREPIGCITPTALIAVMALL
                     ILTAILMLLIAVRSDDEGAIYPAMACLLIVPFLIFVVGFYLYVRKREEELLAGDDEDD
                     EQPASSQQSPEPMSE"
     gene            358..567
                     /locus_tag="VFV93_12585"
     CDS             358..567
                     /locus_tag="VFV93_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166229.1"
                     /translation="MEQTVTIEGIRRYTIHGEPYAVIYYSLAGDRETIHQTQLSEDAL
                     PNGLQVGETAVITRIGTIVAGVHRP"
     gene            complement(725..1219)
                     /locus_tag="VFV93_12590"
     CDS             complement(725..1219)
                     /locus_tag="VFV93_12590"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5166230.1"
                     /translation="MAAESTLTSPNYQNFENFVALDDLADLLRETVQRLQPSAHEISE
                     AWSDAQVTMQQLRVMTILYHEGPTRVSDLARRLGVSTPTITGILDRLVKQRITYRMND
                     PRDRRVVLNNLTHDGRDLVERLMPAQGDSAVLALARLSPDERYALKESLTSLLRALPK
                     QHRS"
     gene            1490..2164
                     /locus_tag="VFV93_12595"
     CDS             1490..2164
                     /locus_tag="VFV93_12595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX5166231.1"
                     /translation="MAQRHTIQASVRTIQGKETKRLRREGQLPGVVYGPVVSDAISVT
                     VDMKELDRMYHAYGSNLLVDLQVDGGTYPVYMRTVTMDRLVRQPQHVEFYAPNMRIEM
                     NASVPVMIVGEPENTDGLLTVARETIDVRGLPDALPSSFEVDVTVLAEIDQAIHVSDL
                     TAPEGVEILTDGEEMIVKLSAPVLQIEPEEEVSEGEIEEAAAAGGVEGAEGVEDTAES
                     EEEPGA"
BASE COUNT          428 a          723 c          773 g          400 t
ORIGIN      
        1 gacgacggcc gcatcggcta cgtccggttg cgcgaagtcg aagcggccat cggcagcgac
       61 acgacgccga cgcgacgtga gccgatcggc tgcatcacgc cgacggcact gatcgccgtc
      121 atggccttgc tgattctgac ggccatcctg atgctgctga tcgccgtgcg ctcggacgat
      181 gagggtgcga tctacccggc gatggcctgc ctgctgatcg tgccgttcct gatcttcgtc
      241 gtcggcttct atctctacgt ccgcaagcgc gaagaggaac tgctggccgg cgacgacgag
      301 gacgacgagc aacctgcctc ctcgcaacag tcccccgaac cgatgagcga gtagatcatg
      361 gagcagaccg tcacgatcga aggcatccgg cgttacacca tccacggcga gccgtacgcg
      421 gtcatctact acagcctcgc cggcgatcgg gagaccatcc accagacaca actgagcgaa
      481 gacgcgctgc cgaacgggct ccaggtcggc gaaacggccg tcatcacccg catcggcacg
      541 atcgtcgccg gcgtccaccg cccataaccc cccgcgtagg gggcggtggc cgcagcctcg
      601 ccctgccccg agcgcgcaac gaagggtgag gctgcggtca tcgcccctac gcgaatggcg
      661 ccgcgcaaac aagacgggga gggcagattg ccctccccga tgacgcacgc gtgatgattg
      721 cggattatga cctgtgttgc ttcggaaggg cgcgcagcag cgaagtcagc gattccttga
      781 gcgcgtagcg ctcgtcgggc gagaggcggg ccagcgcgag gacggcgctg tcgccctggg
      841 cgggcatcag ccgctcgacg aggtcgcgac cgtcgtgggt aaggttgttg agaacgacgc
      901 gccggtcgcg cggatcgttc atccggtagg tgatgcgctg cttgacgagc cggtcgagaa
      961 tgccggtgat cgtcggcgtc gagacaccca gccggcgggc gaggtcggag acgcgcgtcg
     1021 gtccctcatg atagaggatc gtcatcacgc gcaactgctg catcgtgacc tgcgcgtcgg
     1081 accaggcctc ggagatctca tgggcggaag gctgcaggcg ctgtacggtc tcacgcagca
     1141 gatcggccag gtcgtccagc gcgacgaagt tctcgaaatt ctgataattt ggtgaagtca
     1201 gcgtggactc ggccgccatg cagttgccct ttcagccggt tcggatagcc gtttcgcgga
     1261 tcgcaatcgt tactgtcagg atatgtattc ggcgctgctg ggaccatatg ctgttggtcc
     1321 cgtatggaac gatgcgtcgg tacaactgga ccggacaact ggcttggcgg gtacctgcaa
     1381 ctttggtacg gttgtacgtt ggccggtctg gaattcagtg ccggcgtcaa cgcgcgcggt
     1441 ctgcgcgtca cgtcggcatt gcgtaattcc caggaaggtc gatgtattca tggcccagcg
     1501 tcacacgatt caggcgagcg tccgcacgat tcagggcaag gaaaccaagc gtctccgtcg
     1561 cgaagggcag ctgcccggcg tcgtctacgg accggttgtc tccgatgcga tctccgtcac
     1621 cgtcgacatg aaggaactcg accggatgta ccacgcatac ggttcgaacc tgctggtcga
     1681 cctccaggtc gatggcggca cctatccggt ctacatgcga acggtcacga tggaccggct
     1741 ggtccgccag ccgcagcacg ttgagttcta tgcaccaaat atgcggatcg agatgaacgc
     1801 cagcgtcccg gtcatgatcg tcggcgagcc ggaaaacacc gatggactgc tgaccgtcgc
     1861 ccgcgagacg atcgacgttc gcggcctgcc ggacgcgctg ccgtcttcgt tcgaggttga
     1921 cgtcaccgtc ctggctgaga tcgatcaggc catccacgtc agcgacctga cggcgccgga
     1981 aggtgtcgag atcctgaccg atggcgaaga gatgatcgtc aagctgagtg ctccggtgct
     2041 gcagatcgag ccggaagagg aagtcagcga gggcgagatc gaggaagccg ccgctgctgg
     2101 tggtgtcgag ggcgccgagg gcgtcgaaga caccgccgag agcgaagaag aaccgggcgc
     2161 ctagcccttc cgctcgcgac gaaagagcga cccaagatcg ggccgtggca gcacccgccg
     2221 aggcaggtgc gccccggccc gattcgcgtc tgccagcgct gcgtcctttt cgccgacgac
     2281 ctgctccagc tggcgtgcgt aggcggcggt ttccgtgtag aggc
//
LOCUS       DASUGJ010000555         8081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1894588, whole genome shotgun sequence.
ACCESSION   DASUGJ010000555 DASUGJ010000000
VERSION     DASUGJ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894588"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..748
                     /locus_tag="VFV93_12600"
     CDS             332..748
                     /locus_tag="VFV93_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX5166232.1"
                     /translation="MPTREKQFESIRHELEEDLRTTRASLGDMRDETDDLARDSDQEG
                     GVPTNHMADVGTNVYERERLMTFQQEMRDRATAIQDALERMDDGTYCVCERCGKTIPI
                     QRLRAMPFTRYCVEDQEIVDAETADTGLKEEQPLQP"
     gene            complement(873..2087)
                     /locus_tag="VFV93_12605"
     CDS             complement(873..2087)
                     /locus_tag="VFV93_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5166233.1"
                     /translation="MNSRWDSRWSRRGQIAAKMSRPSRTAPAEMISFVYGDPDAGSLP
                     LEEMADAAAWLTEQNRRETLAYANPVGPDGLAVALAEKLKRDYNVEVGPEQILLSTGA
                     AAGLGLIVDMLVDPGDVVLADAPAWMGATGMFRLAGAEVVGVAIDEEGIDPAGVAAAL
                     DRLDAEGKRAKFLYTIPTFQNPTGVELSVERRHALAKLADERGLLILEDDAYIDLRFE
                     GEKKPTIFSLSKNDSVLLFGTLSKTIAAGLRLGWCAGPEDVIAVLSRGRTDSLRNTYT
                     AALAEWFINTGKHAEHITQLRDIYATKCRQMLDALEREMPEGTTWTHPKGGFFIWVTL
                     PEGVDAVDLVTTARENLVDYIPGPSFYSDGSGRRSLRLSFSNVTADEINTGISRLAAT
                     VKAAMPVATTAD"
     gene            complement(2289..2732)
                     /locus_tag="VFV93_12610"
     CDS             complement(2289..2732)
                     /locus_tag="VFV93_12610"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5166234.1"
                     /translation="MVQRPIVQRQRIGPAIRRLRRMNGLTLDDLASQAGISASHLSRL
                     ERGQTLPSFQVLSDIAHVLGADVDEFVRLETEVTERDAEFREILDARGVPDDIQQELM
                     SSSIELRRALIEIFKELPSPKKRGKAGSRNGRGETEVSQPAGMGA"
     gene            3068..4210
                     /locus_tag="VFV93_12615"
     CDS             3068..4210
                     /locus_tag="VFV93_12615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5166235.1"
                     /translation="MRVALLVPSIGVGAEIETPAVRVFAERMVRNVDLHVFTVHYPFA
                     GELAREQRLTIHPAQQNATRFARRLASTIRSISREHRRAPFDLIHALWLHEPGTIAVV
                     AATLLRRPLIASIGGAEVVALPDIAYGALRDPRGRWMTAHVLQRATLVTGGSNYVLRR
                     ARRLVPHRDPDSFRRAPLPIDAAPFAPSGKRVFDPLRPHLLHAASLIPVKNQPTLLRA
                     MRRVVDVIPGARLTIAGGDPLGLRRELEQLGTELGLDGAVTFTGSMRHEQMQTFYRDG
                     DLFVQSSLHESQGLVVLEAGAAGVPTVGTAVGVVADLAPECAVAVRPGDPVALADGIL
                     NLLSDPARLTRMGVRIRQRITDEYDRDSAGDRFLALYDEAIGLPRR"
     gene            4207..5421
                     /locus_tag="VFV93_12620"
     CDS             4207..5421
                     /locus_tag="VFV93_12620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5166236.1"
                     /translation="MKVCYVSAFHPPSISTPAAMLDAWPLLRCLPPELAAEGHEVVAL
                     VHAGQNDERRHDGVRYRFINPGRIAGGIGRLAGRWKPRYGPAYYTPALRLTRALREER
                     PDIVHFSGLTLDVQLAQVAWACSRQRIPLVVHFHGGVPDAGRLQRLQRFNACRVSRVL
                     VTTFEQAQPWLDARLYTCVQFSQVVETSSPFTGIERDEARRATGMAGDPVYLSAGRLD
                     AIKDPLTVLRGFERIVEQQPDARLYCHYLTAEMMPEIVTFLDARPSLAERVALRGRAP
                     LEAMEAIYSSADFLLQASLREWSGLAVIEAMSCGCIPIVSDIPSFRMLTGGSAGKRFP
                     VGDAEALARAALSISHANREALAMACHSRFKQELSFAAMASKISAIYRDVRPDRPNAH
                     QDTPGSGVQPDG"
     gene            5502..6143
                     /locus_tag="VFV93_12625"
     CDS             5502..6143
                     /locus_tag="VFV93_12625"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5166237.1"
                     /translation="MSGRGLLAALGFGLLSLAFDQACDAFKRNGMKRQEALSDGAAHI
                     ATALAVSLPAAPFVQDRQRFVALAALSAVAIDLDHVVAARSTSLIPCMTMPNRPASHS
                     VLTVGIVSYAVERVWPGTQSGLAIVLGLGSHLLRDLATGGAPLFIPRRIIEIPRPPVA
                     SMMLALGVFGRWYARHMLDPRRRRRSNPVVLAPEAMLVSSRALRALREHTRAA"
     gene            6091..7104
                     /locus_tag="VFV93_12630"
     CDS             6091..7104
                     /locus_tag="VFV93_12630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5166238.1"
                     /translation="MSAPAHSARSASTLAPPETSTTLDAASTPLVTAGDVVAIVVNYR
                     TPALTAGAIEHLLASDLPGRRLQVVIVDNNSGDGSVATLAERFPDLGLIVSRQNLGFA
                     GGNNLAIESALRDLPSCVDRNDTFVLLINSDVEVEPDSIATCLAFMEAHPDTGIVGPK
                     VVLPDGRLDLACRRGFPTPWRAFWKLTGLARRFPNNPRFTGYNMTHLDEDQLTEVDAV
                     MGAFMLVRLAAIDAVGLLDERFFMYGEDIDWSFRIKRYGWRVYYVPGARVKHLKGATT
                     RRQSYRMIIEFYRAMWLFHRKHYADRTFFLLNWLVAAGIVLRGGLAIAANALRPADAK
                     RVS"
     gene            7186..>8081
                     /locus_tag="VFV93_12635"
     CDS             7186..>8081
                     /locus_tag="VFV93_12635"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166239.1"
                     /translation="MAKPGPTLATASSRDEAAGRRDGSVAPFQQPERLSRRRDTTRHG
                     LFAILTGIALVAIDALALLIAFISAYNLRDLTNTLGPTTPPPSETYLLLVGLATTAIV
                     AVFALGGTYRQRRTISRMDELYRIVSQVSLGLIIAIAIASLVLGQEFVYSRQMLAYGW
                     MFAVVAVTAGRTLHAGIIGRLRARGVASDRLLIVGAGPTGRLILDKVRRSPNLGFDVV
                     GFVRHSPFDGELPPELDGVPILGMSSQIGEIVEAHAVDEIIVALSGTPNEEILDLVYA
                     VTQLPVAIRVYPDSFRLLTSDSL"
BASE COUNT         1381 a         2640 c         2608 g         1452 t
ORIGIN      
        1 cgctttggcc gacgccgccg gtgcgatcat ccgcagagta ccctctgcaa cacggtacca
       61 cataatcgtg ttttgcaggg caccgtccgg caacgacagc gccagtgccg caaatgcttg
      121 cgaccgcaac agctccagat cgtcgtcgtg aaacatcgac attatgtcca gtcttccttc
      181 tgcggtgacc agccgtccgc gagggacagg catggacatt gcaactgcgt tactggccgg
      241 cccgcgattg ggacacggcg caaccattat ctgacaggcg gaagtagacc gccgcgtgag
      301 cgtcatgcaa cgcaagaatg cgaggcagtt catgccaaca cgggagaaac agttcgagtc
      361 aatcaggcac gagctggaag aggatctacg caccacccgc gcgtcgctcg gggatatgag
      421 agacgagacc gacgaccttg cccgcgacag cgaccaggaa ggcggcgtcc cgaccaacca
      481 catggccgac gtcggcacca acgtctacga acgcgagcgc ctgatgacgt ttcagcagga
      541 gatgcgcgac cgggcgaccg ccatccagga cgcgttggag cgcatggacg acggcaccta
      601 ctgcgtctgc gagcgctgcg gcaagacgat ccccatccag cgcctgcgcg cgatgccctt
      661 cacccgctac tgcgtcgagg atcaggaaat cgtcgatgcc gaaacggccg acaccggcct
      721 aaaggaggaa cagccgcttc agccgtaaac accgttgtcg tcctgagtgg ttcaccacac
      781 tggttgtgtc gcagcgaagg acctctccgc gtgagccaca tgggcggtca aggcggagag
      841 gtccttcgct gcggctttat acgccagcgg ttctagtcgg ctgtcgtcgc gaccggcatg
      901 gcggccttga cggttgcggc gaggcggctg atgccggtgt tgatctcgtc ggcggtcacg
      961 ttcgagaacg agagccgcag cgatcggcgg ccggagccgt ctgagtagaa cgacggtccc
     1021 gggatgtagt caaccaggtt ctcgcgcgct gtcgtgacca gatcgacagc gtcgacaccc
     1081 tccggcagcg tgacccagat gaagaagcca cccttgggat gcgtccaggt cgtgccttct
     1141 ggcatctcgc gttccagcgc gtcgagcatc tgacggcact tggttgcgta gatgtctcgc
     1201 agctgcgtga tgtgctcggc gtgcttgccg gtgttgatga accactcggc cagcgcggcc
     1261 gtgtaggtat tgcgcaggct gtcggttcgg ccgcgcgaca ggacggcgat cacatcttcg
     1321 ggacctgcgc accagccgag acgcaggccg gccgcgatcg tcttcgagag cgtcccgaag
     1381 agcaggaccg agtcgttctt cgacaggctg aagatggtcg gcttcttctc gccttcgaag
     1441 cgcaggtcga tgtaggcgtc gtcctccagg atcagcagcc cgcgctcgtc ggcgagcttc
     1501 gccagggcgt gccgtcgctc gacggagagc tcgacgccgg tcgggttctg gaaggtcggg
     1561 atcgtgtaga ggaacttggc acgcttgccc tcggcgtcga gccgatcgag ggcggccgcg
     1621 acgccggccg ggtcgattcc ttcctcgtcg atcgcgacgc cgacgacttc tgcgccggcc
     1681 agccggaaca tgccggtcgc gcccatccag gctggcgcgt cggccagaac gacgtcgccg
     1741 ggatcgacca gcatgtcgac gatcaggccg aggccggcag ccgcgccggt cgagagcagg
     1801 atctgctctg ggccgacctc gacgttgtag tcacgcttca gcttctcggc cagtgcaacg
     1861 gcgaggccgt ccggaccgac cgggttggcg taggccagcg tctcgcggcg gttctgctcg
     1921 gtgagccagg cagcggcgtc ggccatctcc tcaagcggga gcgaaccggc gtccgggtcc
     1981 ccatagacga acgaaatcat ctcggccggc gccgtccgcg acgggcgcga catcttcgcg
     2041 gcgatctgtc cacgccgcga ccagcggctg tcccaacgtg aattcatcag ctggttccct
     2101 ctccggttcg gcccggactg cgcgaccgcc agccaactgc ggccgatctg aaacatcaac
     2161 gaccgaacga gtatatccca gcgtctggta tgggcggcag caacagtgaa ccccccgggg
     2221 tcagcataac cagctgtgat cctggagggt tcctacgagc tacctggcgg gtgaagggca
     2281 ggcctgaatc aggcgcccat cccggccggt tgcgacactt ccgtctcgcc acggccattc
     2341 cttgagcctg ccttgccacg cttcttcggg ctcggcagtt ccttgaagat ctcaatcaac
     2401 gcacgtcgga gctcgattga gctcgacatc agttcctgct ggatgtcgtc gggaacaccg
     2461 cgtgcgtcga gaatctcgcg gaattcggcg tcccgctcgg tgacctcggt ctcgagccgg
     2521 acgaactcgt cgacatcggc gcccagcaca tgggcgatgt cggagagcac ctgaaagctt
     2581 ggcagcgtct gaccacgctc caggcggctc aggtgcgatg ccgagatgcc ggcctgcgac
     2641 gcgagatcat ccagcgtgag cccgttcatc cgtcgcagcc ggcggattgc cgggccaatt
     2701 ctctgtcgtt gtacgatggg tcgttgaacc atgatacgtc ctctttccgc gcaaatccga
     2761 atgatgcaga cgtgttcaac cgcgaccgtg gcggcgggcg gcaacgaacc ctggacagtc
     2821 ccccgactgg tgcaacgcca tcctcccgaa tgcaccagac atcgcgaacg tcagagcttc
     2881 actaggttat acagcgacca tccgcgattt ggaagaggga aatgagcaaa ctgaaccttc
     2941 cgcgcatacg aaaatccgac ctttacaatt tctcaaccgg cgaagcaggc ccgtgatcgg
     3001 cgacatcatg ttacaaccag tgaaatggca acaggacacg ctgtcctgct accgtgaagg
     3061 gcgagcggtg agagtagcac ttctggtacc aagcatcggt gttggtgctg agatcgagac
     3121 tccggcggtc cgggtatttg ctgaacgaat ggtcagaaac gtcgatcttc acgtcttcac
     3181 ggttcactat ccgtttgccg gtgaactcgc ccgcgaacag cggctcacga ttcaccccgc
     3241 gcaacagaat gccactcgct tcgcgcgccg gctggcgagc acaatcagat cgatctcccg
     3301 cgaacaccga cgcgcgccgt tcgacctcat ccacgccctc tggctccacg agccgggcac
     3361 aatcgccgtc gtcgctgcaa cactcctgcg cagaccgctg atcgcgagta tcggtggcgc
     3421 cgaggtcgtg gccctcccgg acatcgcata cggcgcgctg cgggacccgc gcgggcgatg
     3481 gatgacggcg cacgtgctcc agcgcgcgac gctcgtgacc ggcggttcga actacgttct
     3541 gcggcgagcg cgacgactcg tgccacaccg cgaccctgac agtttccgcc gagcaccgct
     3601 gccgatcgac gcggctccgt tcgcaccgtc aggcaagcga gtgtttgacc cgcttcggcc
     3661 gcacctgctg cacgcggcgt cgctgattcc cgtcaagaac caaccaacgc tgctgcgtgc
     3721 gatgcgccgg gtcgtcgacg tgattcctgg cgcacggttg acgatcgccg gtggcgatcc
     3781 gctggggctg cgtcgtgagc tggagcaact gggcacggag ctggggctgg acggtgcagt
     3841 gacgttcact ggctcgatgc gacacgaaca gatgcagacg ttttatcgag acggcgacct
     3901 gttcgtgcag tcgagtctcc atgagagtca ggggctggtc gtgctcgaag ccggcgcggc
     3961 tggtgtgccg acggtcggga cggccgttgg cgtcgtggca gatctggcgc cagaatgtgc
     4021 ggtggcagtt cgtcccggcg acccagttgc gctggctgat ggcatcctca atctcctgtc
     4081 cgatccggcc cggctgactc gcatgggagt gcgcatccgc cagcggatca ctgacgaata
     4141 cgatagggat agcgcaggcg acaggtttct cgcgctctac gacgaagcga tcgggcttcc
     4201 gcgtcgatga aggtctgcta cgtctccgcc tttcacccgc cgtcgatctc gacgccagcg
     4261 gcgatgctgg atgcctggcc gttgttgcgt tgcctgccgc cggagttggc ggcggagggg
     4321 catgaggtcg tcgcactggt gcatgccggc cagaatgacg agcgacggca cgacggcgtt
     4381 cgctaccgct tcatcaaccc cgggcgcatc gcgggcggca tcggtcgttt ggccggcaga
     4441 tggaagccac gctacgggcc ggcctattac acgcccgcgc ttcggctgac gcgcgcgttg
     4501 cgcgaggaac ggcccgacat cgtccacttt tccggcctga cgttggacgt gcagctcgcg
     4561 caggttgcct gggcctgttc ccggcagagg atcccgctgg tcgtccattt ccacggcggc
     4621 gtacccgacg ctggccgcct acaacgcctc cagcggttca acgcctgtcg agtcagccgt
     4681 gtcctcgtga cgacgttcga gcaggcgcag ccgtggctcg atgccaggct gtacacgtgt
     4741 gtccagttca gccaggtcgt ggaaacgagt agtccgttca ccggaatcga gcgcgacgag
     4801 gcgcgccgcg cgaccgggat ggccggcgat ccggtctatc tgtcggcagg gcggctggat
     4861 gcgatcaagg acccgttgac ggtgctgcgt ggcttcgagc gaattgtgga gcagcaaccg
     4921 gacgcgcggc tgtactgcca ttacctcacc gccgagatga tgccggagat cgtgacgttt
     4981 ctcgacgcac gcccatccct tgccgaacgg gttgcgttgc gcggccgggc tccactggag
     5041 gcgatggagg cgatctactc ttcagccgac ttcctgctgc aggctagcct gcgtgagtgg
     5101 agcgggctgg ccgtcatcga ggcgatgtcg tgtggctgta ttccgatcgt cagcgatatc
     5161 ccctcgttcc ggatgctgac aggcgggagc gcgggaaaac ggtttccggt tggagacgca
     5221 gaggcgctgg cgcgagcggc actgtccatc tcgcatgcga accgcgaggc gttggcgatg
     5281 gcatgtcatt cgcgattcaa gcaggagctg tcgttcgcgg cgatggcgag caagatcagc
     5341 gcgatctacc gtgacgtgcg acccgatcgc ccgaatgccc atcaggacac gccaggcagc
     5401 ggcgttcagc ctgacggatg agtacaatag aggccatgac gacacgactt cggacgacga
     5461 caatctcctt caagacccga cggccgatgg ctcgacgcac gttgagcggt cgaggcctgc
     5521 tggcggcact tggattcggg ctcctctcgc tggcgtttga ccaggcctgc gatgccttca
     5581 agcgcaacgg catgaagcgg caggaggcgc tcagcgacgg cgccgcccat atcgcaacgg
     5641 cactggctgt ctcgttgccg gccgcgccgt tcgtccagga tcgccagcgg ttcgtcgcgc
     5701 tggcagccct cagcgccgtc gcgatcgatc tcgaccatgt cgtcgccgcc cggtcgacca
     5761 gcctgattcc ctgcatgacg atgcccaacc ggccggcctc gcacagcgtc ctgacggtcg
     5821 gtatcgtcag ctacgccgtc gagcgcgtct ggcccggcac gcagtcgggt ctggcgatcg
     5881 tccttgggct gggctcgcac ctgctgcgcg atctcgcaac tggtggcgcg ccgttgttca
     5941 ttccccgtcg catcattgag atcccacgtc cgccggtcgc gtcgatgatg ctcgcgctcg
     6001 gtgtctttgg ccgctggtac gcccgccaca tgctcgatcc acgacggcgg cggcgttcca
     6061 acccggtcgt gcttgccccg gaggctatgc ttgtcagctc ccgcgcactc cgcgcgctcc
     6121 gcgagcacac tcgcgccgcc tgagacatcg acaacgctcg acgccgcatc aacaccactg
     6181 gtcactgccg gtgacgttgt cgccattgtc gtgaattacc gaacacccgc actcactgcc
     6241 ggcgcgatcg agcacctgct cgccagcgac ctccccggcc ggcggctcca agtcgtcatc
     6301 gtcgacaaca actccggcga cggcagcgtc gcgacgctgg ccgaacgctt ccctgatctg
     6361 gggctgatcg tttcacggca gaatctcggc tttgccggcg gcaacaacct ggcgatcgaa
     6421 tcggccctgc gcgacctgcc cagttgcgtg gatcgcaacg acacgttcgt cctgctgatc
     6481 aacagcgatg tcgaggtcga gccggactcg atcgcgacct gtctggcctt tatggaagcg
     6541 cacccggaca ccggcatcgt tggcccgaag gtcgtcctgc ccgacgggcg gctcgatctg
     6601 gcctgccggc gcgggttccc gacgccgtgg cgcgcattct ggaagctgac cggcctggcc
     6661 cggcgatttc cgaacaaccc gcgtttcacg ggttacaaca tgacccatct cgacgaagac
     6721 cagttgacgg aagtcgacgc tgtgatgggc gccttcatgc tggtcaggct ggccgcgatc
     6781 gacgcggttg ggctgctgga cgagcgtttc ttcatgtacg gcgaagatat cgactggtcg
     6841 tttcgaatca aacgatatgg ctggcgcgtc tactacgtgc cgggggcgcg ggtgaagcat
     6901 ctcaagggcg cgacgacgcg gcggcagtca taccggatga tcatcgagtt ttaccgggcg
     6961 atgtggctgt ttcaccgcaa acactacgcg gatcggacgt ttttcctgct aaactggctt
     7021 gttgctgcag gaatcgtcct gcgcggtggg ctggcgattg cggcgaacgc gcttcgacca
     7081 gctgatgcga aacgcgtctc ctgaacgtca tgccgttcgc gaacgacagt cagtcgcaaa
     7141 cgcccgcgta cgaaccgcaa ctggcggaat gaggagcagt gacggatggc gaaacccggc
     7201 ccaacgctcg cgacggcatc gtcgcgggac gaggctgcgg ggagacggga cggcagcgtc
     7261 gcgccattcc agcaaccgga acggctgtcg cggcggcgtg acacgacccg gcacggcctg
     7321 ttcgcgatcc tgaccgggat cgcgctggtt gcgatcgatg cgctcgccct gctgatcgcc
     7381 ttcatcagtg cctacaacct gcgcgacctg accaacacgc tcggcccgac gacgccgccg
     7441 ccgagcgaga cgtacctgct gctggtcggg cttgcgacga ccgctatcgt cgccgtcttc
     7501 gcgctgggtg gcacctaccg ccagcgccgc acgatctccc ggatggacga gctctaccgg
     7561 atcgtctcgc aggtctcgtt gggtctcatc atcgcgatag cgatcgcgtc gctggtgctg
     7621 gggcaggagt tcgtctattc acgtcagatg ctggcctacg gctggatgtt cgcggtcgtg
     7681 gcggtgacgg ccggtcggac gctccacgcc ggcatcatcg gccgcctgcg cgcccgcggt
     7741 gtcgccagcg accggctgct gatcgtcggg gcaggaccaa cggggcgctt gattctggac
     7801 aaggtccggc gctcgcccaa tctcggcttc gacgtcgtcg gcttcgtccg ccactcgccg
     7861 ttcgacggcg agctgccgcc cgagctcgac ggcgtgccga tcctcggcat gtccagccag
     7921 atcggcgaga tcgtcgaagc ccatgccgtt gacgagatca tcgtcgccct ctccggcacg
     7981 ccgaatgagg agatcctgga cctcgtatac gccgtgacgc aactgccggt cgcgatccgg
     8041 gtctatccgg actcgttccg gctgctgacg agcgacagcc t
//
LOCUS       DASUGJ010000556         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1756599, whole genome shotgun sequence.
ACCESSION   DASUGJ010000556 DASUGJ010000000
VERSION     DASUGJ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756599"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1458
                     /gene="purB"
                     /locus_tag="VFV93_12640"
     CDS             124..1458
                     /gene="purB"
                     /locus_tag="VFV93_12640"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX5166240.1"
                     /translation="MIDRYTLPEMGAIWGEQRKIDHWLAVEIAVCEAWAKRGVIPADA
                     MPAIRGASCDLQRMKEIERETDHDVIAFLRATGETVGDAARFIHLGLTSSDVIDTALA
                     AQVVEAADLLLRRLDDAIDVVGKQAVAHRETLMIGRTHGVHAEPITFGFKLAVWYDEL
                     RRARTRLAAARTAMAVGKISGAVGTHANVPPDVEEDVCDYLGLGVDPVSTQIIQRDRH
                     AEFITALAVLASSLDKFAVEIRHLARTEVRELEEPFNPGNQGSSAMPHKRNPHESERL
                     SGLARLVRGYAVTAMENVVLWHERDISNSSAERVIFPDACILVDYMLHLFTDLVTNWV
                     VYPERMHANLESTGGAIFSQRAMLALVEAGLDRQVAYKIIQKNAMRAWDEGGHLRDYL
                     RDEPEVTSRLSNQQIDELFDYGYHLRHVDVALERLGLLQPATLAGAGGGGNL"
     gene            1455..2351
                     /locus_tag="VFV93_12645"
     CDS             1455..2351
                     /locus_tag="VFV93_12645"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX5166241.1"
                     /translation="MTTALSEIQIGGLRRFRRGKVRDTYDLGDKLLMVASDRMSAFDV
                     VLPDAIPDKGRVLTQLSRFWFERTGDVVPNHLISADVADLPDEVGPAAELLRGRFMIV
                     KKAERIDIECVVRGYLAGSGWAEYRKSLTVCGEKLPEGLTESARLSEPIFTPAAKVDE
                     GHDENISYDKMKQIVGKDLAEQLRDASMRLYQFAEQHARTRGIIMADTKFEFGMVDGK
                     LIVIDEILTPDSSRFWDAAQYEPGRSQASFDKQPLRDWLERSGWDKNPPGPPLPPEII
                     EETAARYRKAYEIVTGMELPSA"
     gene            2348..2647
                     /gene="purS"
                     /locus_tag="VFV93_12650"
     CDS             2348..2647
                     /gene="purS"
                     /locus_tag="VFV93_12650"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX5166242.1"
                     /translation="MSTQLTDDTTTTQRSYLAEVFVSLKPVVNDPQGLAIRDGLHALG
                     YRGVEAVRAGKYLRVTLEASSADDAETQVARMCDQLLTNPVIETYQISITALADH"
     gene            2680..>3081
                     /locus_tag="VFV93_12655"
     CDS             2680..>3081
                     /locus_tag="VFV93_12655"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX5166243.1"
                     /translation="MRFGVVIFPGSNGDHDALTAVELGLHEEVVPIWHKDTSIGNCDV
                     LLIPGGFTYGDYLRAGAIARFSPIMAAVEEFANQGGPVLGICNGFQILTEAGLLPGAL
                     LKNSELTFRCDWINVRVESDRTPFTATLDPGT"
BASE COUNT          551 a          998 c          995 g          537 t
ORIGIN      
        1 ccgggcgcgc gtccataccc cgcaaccacg agccaaccgc cgccgcgagg cggcattctc
       61 atttcggcgc gtgctactag cgtcgacgta cctgtttgcc tggaacgtgg atagggagcc
      121 atcgtgatcg atcgatacac cctgccggag atgggcgcga tctggggcga gcagcgcaag
      181 atcgatcact ggcttgcggt cgagattgcc gtctgcgaag cctgggccaa gcgcggcgtc
      241 attcccgccg acgcgatgcc ggccatccgt ggcgcctcgt gtgacctcca gcgcatgaag
      301 gagatcgagc gcgagaccga ccacgatgtc atcgccttcc tgcgagccac cggcgagacg
      361 gttggagacg ccgcccgctt cattcacctc ggcctgacca gctcggacgt gatcgacaca
      421 gcgcttgccg ctcaggtcgt cgaggcagcc gatctgctgc tacgccgcct cgacgacgcg
      481 atcgacgttg tcggcaagca agcggtcgcc caccgcgaga cgctgatgat tggccgaacg
      541 cacggcgtcc acgccgagcc gatcaccttc ggcttcaagc ttgccgtctg gtacgacgag
      601 ttgcgccgcg cccggacgcg gctggcggct gcacggacgg cgatggccgt cggcaagatc
      661 tccggtgctg tcggcaccca tgccaatgtc ccgccggatg tcgaagagga tgtttgcgac
      721 tacctcggcc tcggcgtcga cccagtctcg acccagatca tccagcgcga ccgccatgcc
      781 gagttcatta cggcgttggc agtcctcgcc tcttcgctcg acaagttcgc ggttgagatt
      841 cgtcatctgg cgcgaactga ggtccgcgag cttgaggagc cgttcaatcc cggcaatcag
      901 gggtcgtcgg ccatgccaca caagcgcaac ccgcatgaga gcgaacgcct cagcggcctg
      961 gcccggctgg tacgcggcta cgccgtgacc gcgatggaga acgtcgtcct ctggcacgag
     1021 cgcgacatct cgaactcgtc cgccgagcgc gtgatcttcc ctgacgcctg catcctggtc
     1081 gactacatgc tgcatctgtt cacggacctc gtgaccaact gggttgtcta tccggagcgg
     1141 atgcacgcca atctggaatc gaccggcggg gcgatcttct cgcagcgggc gatgctggcg
     1201 ctggtcgagg ccggcctcga tcgccaggtc gcctacaaga tcatccagaa gaatgcgatg
     1261 cgcgcctggg atgaaggcgg ccacctgcgc gactacttgc gcgatgagcc ggaggtaacg
     1321 tcccggctct ccaaccagca gatcgatgag ttgttcgatt acggctatca cctgcggcac
     1381 gtcgatgtcg cgctggagcg gctcggtctg ctccagccgg cgacgctggc cggcgcgggt
     1441 gggggaggga atctgtgacg acggcactga gcgagattca gatcggcggc ctacgccggt
     1501 tccgcagggg caaggtccgc gatacctatg atctcggtga caagctactc atggttgcct
     1561 cggaccgaat gtcggcgttc gacgttgttc tgccggatgc gatccccgac aagggccgcg
     1621 tcctcacgca gctctcgcgc ttctggttcg agcgaaccgg cgacgtcgtt cccaaccacc
     1681 tgatctcggc ggatgtcgcc gatctgccgg acgaagtcgg cccagcagcc gagttgctgc
     1741 gcggacgctt catgatcgtc aagaaggccg agcgaatcga catcgagtgc gtcgtccggg
     1801 gttacttggc cggctccggc tgggccgagt accgcaagag cctgacggtc tgcggcgaga
     1861 agctgcccga aggtctgacg gagagcgccc ggctgtcgga accgatcttc acgccggccg
     1921 ccaaagtcga cgaggggcac gacgagaaca tctcctacga caagatgaag cagattgtcg
     1981 gcaaggacct cgccgagcag ttgcgcgacg cctcaatgcg gctctaccag ttcgccgagc
     2041 agcacgcgcg gacgcgcggc atcatcatgg cagataccaa gttcgagttc gggatggtcg
     2101 acgggaagct gatcgtcatt gacgagatcc tgacgccgga ctcgtcccgc ttctgggacg
     2161 ccgcccagta tgagccgggc cgctcgcagg ccagcttcga caagcagccg ctgcgcgact
     2221 ggctggaacg ctccggctgg gacaagaacc cgccgggacc gccgctgccg cccgagatca
     2281 tcgaggagac ggctgcccgc tatcgcaagg cgtacgagat cgtcactgga atggagctac
     2341 ccagcgcgtg agcacgcagt tgaccgacga cacgacaacg acgcagcgct cgtacctggc
     2401 cgaggtgttc gtctcgctca agccggtcgt caacgatccg caagggttgg cgattcgcga
     2461 cgggctgcac gccctcggct accgtggcgt cgaggcggtt cgcgccggca agtacctgcg
     2521 tgtcacgctc gaagccagct cagccgatga cgccgagacg caggtcgccc gaatgtgtga
     2581 ccagttgcta accaacccgg tcatcgagac gtatcagatt tcaatcacgg ccctggccga
     2641 tcattagagt tgcgactgga acgcggaggg ttctgagacg tgcgctttgg agtagtgatc
     2701 tttcccggct cgaatgggga ccacgacgcg ctgacggcgg tcgagcttgg cctgcacgaa
     2761 gaagttgtcc cgatctggca caaggacacg tcgatcggga attgcgacgt gctgctgatt
     2821 cccggcggct tcacctatgg tgactacctg cgggccggcg cgatcgcccg cttctccccg
     2881 atcatggctg ccgttgagga gtttgccaac cagggcggcc cggtgctcgg catctgcaac
     2941 ggctttcaga ttctgaccga agctggactg ctgcctgggg cgctgctcaa gaactccgaa
     3001 ctgacgttcc gctgcgactg gatcaacgtc cgcgtcgagt cggaccggac gccgtttacg
     3061 gcgacgctgg atcccggcac a
//
LOCUS       DASUGJ010000557         3837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_923933, whole genome shotgun sequence.
ACCESSION   DASUGJ010000557 DASUGJ010000000
VERSION     DASUGJ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923933"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="VFV93_12660"
     CDS             complement(<1..1303)
                     /locus_tag="VFV93_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5166244.1"
                     /translation="MDDLLRATSERAAHYLAGLADRAVAPAPEAIARLGTLGGPLPDG
                     PSDPADVIGLLDGIGSPATTATAGGRYFGFVIGGTLPAALAASWLATTWDNDAGMSLV
                     SPVGAAVEEIARSWLLDVLRLPADSGIGFVTGGTMANLTCLAAARMAVLARVGWDVEA
                     DGLFGAPPVTVVVGAEAHISVFKALGLLGFGRERVLRVPVDDQGRMLVEELHRLDGPS
                     IVCAQAGNVNSGAVDPIGAIAERVHRDDAWLHIDGAFGLWAAAAPERAHLVAGADAAD
                     SWAIDGHKWLNVPYDSGIAIVRDAGALRRAMAASASYLVASDNREPDQYTPEMSRRAR
                     GVEAWAALRSLGRSGLADLVERCCRHATRFADGLASAGYQILNEVTLNQVLVSFGDDA
                     TTRRVIAAIQADGTCWCGGTTWQGRAAMRISVSSWATTDEDA"
     gene            complement(1347..1658)
                     /locus_tag="VFV93_12665"
     CDS             complement(1347..1658)
                     /locus_tag="VFV93_12665"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5166245.1"
                     /translation="MIDRVWAARATPANAPAYVEHLRDHVLPTLRRVDGYEGARLLQR
                     LDGGDVEMVVVTRWRSLASIRGFAGDDIERAVVADEAAVLLTGFDDRVAHFEVVLTDD
                     R"
     gene            complement(1655..1939)
                     /locus_tag="VFV93_12670"
     CDS             complement(1655..1939)
                     /locus_tag="VFV93_12670"
                     /inference="COORDINATES: protein motif:HMM:NF009508.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HEX5166246.1"
                     /translation="MQYFALVYDLVDDYVARRTVFRDAHLKLATAAHERGEIVLAGAL
                     ADPVDRALLVFRAGDASVVEEFARNDPYVTNGLVTHWEVRPWTVVIGGES"
     gene            2052..2939
                     /locus_tag="VFV93_12675"
     CDS             2052..2939
                     /locus_tag="VFV93_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5166247.1"
                     /translation="MTGYPATADQLHFGIVTGQHRRPLEQIEEHWRYAEETGWDSAWA
                     FDHFFSLGKSEMEQTLEGWTLLAALAAKTSRVQLGLMVTGNTHRNPAILAKQAVTVDH
                     ISNGRLILGLGAGWQEREHQAYGIDFPPPRERVDRLGEALEMFRLLETQERTTFNGRY
                     YRLDNAPFEPKPIHGHIPILIGTAGQRMLRHVARYADLWDGGEDPGAYAANAERLRVI
                     CNEIGRDPAEIRLVFSAYRQPLESVDAFRDHVTRYAKIGVRTFLFNTPFSTLPPLFDD
                     LAQRVVPELREQFAAGELG"
     gene            3027..3545
                     /locus_tag="VFV93_12680"
     CDS             3027..3545
                     /locus_tag="VFV93_12680"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5166248.1"
                     /translation="MLAEAIRNSYAYNEWATEKIFDAAEGMTVEQLDAPGPIPHGSIR
                     GTLLHMLTAHQRFLSWWDGSMTAQESYSRRMNPDDYPDLAAVRALWQQVAEQARAFVG
                     DLSDEDATHYLSMTRPDGAEFGFPLWQMMQHIANHATQHCSEIAAMLTEAGRSPGDID
                     MLFYQIEANARA"
     gene            3595..>3837
                     /locus_tag="VFV93_12685"
     CDS             3595..>3837
                     /locus_tag="VFV93_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166249.1"
                     /translation="MIAFVPYTQQSPELEDALRIYGRVWPDRDPEQSREGFTRYAGYP
                     DFRGFLACVDGEPVGVGYGARSFPGVPWHDLVAPRLG"
BASE COUNT          681 a         1361 c         1245 g          550 t
ORIGIN      
        1 cggcatcttc atcagtcgtc gcccaggacg agacgctgat ccgcatcgcg gcccggccct
       61 gccaggttgt gccgccgcac cagcaggtgc cgtcggcctg gatcgcggca atgacgcgac
      121 gggtggtggc gtcgtcgccg aaggagacca gcacctgatt gagcgttacc tcgttgagga
      181 tctggtagcc ggctgatgcg agtccgtcag cgaagcgggt cgcgtggcgg cagcagcgct
      241 ccaccaggtc ggccaggccg gagcggccca gcgagcggag cgccgcccag gcctcgacgc
      301 cgcgcgcccg gcgtgacatc tccggtgtgt actggtccgg ctcgcggttg tcactggcga
      361 cgaggtagct ggcgctggcc gccatcgccc gccgcagcgc cccggcatcg cggacgattg
      421 cgatgccgct gtcgtagggg acgttgagcc acttgtgccc gtcgatcgcc caggagtcgg
      481 cggcgtcggc accggcgacc agatgagccc gctccggcgc ggctgccgcc cagagcccga
      541 acgcgccatc gatatgcagc caggcgtcgt cccgatgtac acgctcggca atcgcgccga
      601 tcggatcgac agcgccgctg ttgacgttgc cggcctgggc gcagacgatc gaggggccgt
      661 cgagacggtg aagctcctcg accagcatcc gcccctgatc atcgacggga acgcgtagca
      721 cccgctcgcg gccgaatccg agcagcccaa gcgccttgaa gacggagatg tgcgcctccg
      781 caccgacgac gaccgtcacc ggcggcgcac cgaacaggcc gtccgcctcg acatcccagc
      841 cgacccgcgc caacacggcc atccgggcgg ctgccaggca ggtgaggttc gccatcgtcc
      901 cgccggtgac gaagccgata cctgaatcgg cagggagccg gagaacatcc agcagccagc
      961 tgcgcgcgat ctcctcgaca gccgcgccga ccggcgagac gagcgacatc ccggcgtcgt
     1021 tgtcccaagt cgtcgccagc cagcttgcag ccagcgcggc cggcagcgtc ccgccaatca
     1081 cgaacccgaa gtagcgcccg ccggccgttg ctgtcgtcgc tggggagccg atcccgtcca
     1141 gtaacccgat cacgtcagcg ggatcgctgg gaccatccgg caacggcccg cccaacgtgc
     1201 ctaaacgcgc gatcgcttcc ggtgccggag caacggcgcg atcggcaagg ccggccaggt
     1261 agtgcgcggc acgctcgctc gtcgcgcgca gcagatcgtc catcagcgcg agcctttcag
     1321 tgtggggacc tcgccggcga acgagactat cgatcgtccg tcagcacgac ctcgaagtgg
     1381 gcgacgcggt catcgaaccc ggtcaggagc accgcagcct catcggcgac gaccgcccgc
     1441 tcgatgtcgt caccggcgaa gccgcgaatc gaggccagcg accgccagcg cgtcacgacg
     1501 accatctcaa cgtcgccgcc gtccaagcgc tgcaagagcc gcgcgccctc gtaaccgtcc
     1561 acgcgacgca gggtgggcag aacgtggtcg cgcaggtgct cgacataggc gggcgcgttc
     1621 gccggcgtcg ccctggccgc ccagacacgg tcaatcacga ctcaccgccg atcacgactg
     1681 tccagggacg gacttcccag tgggtcacga gcccgttggt gacgtacggg tcgttgcggg
     1741 cgaactcctc aacgaccgag gcgtcgccag cgcggaagac gagcagtgcc cggtcaaccg
     1801 ggtccgccaa cgcaccggcc aggacgatct cgccccgctc gtgagcagcc gtcgcgagtt
     1861 tgaggtgggc atcgcgaaag acggttcgcc gcgcgacata atcatcaacc agatcgtaca
     1921 ccagcgcgaa gtactgcatg ctgaccctcc ccgtcatgcg ctcgaaacag cgcaacgtta
     1981 ccagtccgga gcccgagcgc gtatcctgtt gggacgcgat cgagagccat cagcgcggac
     2041 ggaggggatt gatgacagga tacccagcaa cagccgacca gcttcacttc gggatcgtca
     2101 ccggccagca tcgccggcct ctggagcaga tcgaggagca ctggcgctac gccgaagaga
     2161 ccggctggga ttcggcctgg gcctttgacc acttcttctc gctcggcaag tccgagatgg
     2221 aacagaccct cgaaggctgg acgctgctgg cagcgctggc cgcgaagacc tcacgggtcc
     2281 agctcgggct gatggtcacc ggcaacacgc accgcaaccc ggccatcctc gccaagcagg
     2341 ccgtgacggt cgaccatatc tccaacggcc ggctgatcct cgggctcggc gccggctggc
     2401 aggagcgcga gcaccaggcc tacgggatcg acttcccgcc cccacgcgag cgggtcgacc
     2461 ggctcggtga ggcgttggag atgttccggc tgctggagac acaggaacgg acgaccttca
     2521 acggccgcta ctaccgactc gacaacgcgc cgttcgagcc gaagccaatc cacggccaca
     2581 tcccgatcct gatcggaacg gccggccagc ggatgctgcg ccacgtcgcc cgctacgccg
     2641 acctctggga cggtggcgag gaccccggcg cctacgccgc gaacgccgag cgattgcgcg
     2701 tcatctgcaa cgagatcggc cgcgacccgg ccgagatccg gctcgtcttc tccgcctacc
     2761 gccagccact tgaatcggtc gacgccttcc gagaccacgt cacgcgctac gcgaagatcg
     2821 gtgtccgcac gttcctgttc aacaccccgt tcagcacgct cccacccctc ttcgacgacc
     2881 tcgcccagcg cgttgtcccg gaactgcgcg aacagttcgc ggccggggag ttggggtgat
     2941 gcgtgacgcg tgatgcgtga cgcgagagac gaagataaca cgacttcgcg tcacgcatca
     3001 cgcgtcaccc tcctgaaagg agacccatgc tggcagaggc catccgcaac agctacgcgt
     3061 acaacgagtg ggcgacggag aagatcttcg acgccgctga gggaatgacg gtggaacagc
     3121 ttgacgcgcc ggggccgatc ccgcatggct cgatccgcgg gacgctgttg cacatgctca
     3181 ccgcccacca gcgcttcctg tcgtggtggg atggctcgat gacagcccag gagtcctaca
     3241 gccggcgcat gaatccggac gactaccccg acctggcggc cgtccgggca ctctggcagc
     3301 aggttgcgga acaggcgcgg gcattcgtcg gcgacctgag cgacgaggat gccacgcact
     3361 acctctcgat gacccgccca gatggcgcgg agtttggctt tccgctctgg cagatgatgc
     3421 agcacatcgc caaccacgcc acccagcact gcagcgagat cgccgccatg ctgaccgagg
     3481 ccggccgatc acccggcgac atcgacatgc tcttctatca aatagaagcc aacgcccggg
     3541 cttgatcgct gccggcggtc atcagtgccc ggtgaacgga acgctggaac gacgatgatc
     3601 gcgttcgtgc catatacaca gcaatcgccg gagcttgagg acgcgctccg gatctatggc
     3661 cgggtctggc ccgaccgtga tccggagcag tcgcgcgagg ggttcacccg ctacgccggc
     3721 tatccggact tccggggctt cctcgcctgc gtcgatggcg agccggtcgg cgtcggctac
     3781 ggggcacgct cgtttcccgg cgtgccctgg cacgatctcg tcgcgccccg gctgggg
//
LOCUS       DASUGJ010000558         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_923951, whole genome shotgun sequence.
ACCESSION   DASUGJ010000558 DASUGJ010000000
VERSION     DASUGJ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923951"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1122)
                     /locus_tag="VFV93_12690"
     CDS             complement(79..1122)
                     /locus_tag="VFV93_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX5166250.1"
                     /translation="MPDRLIDLSSDTATKPSREMRAFMADAEVGDEQRREDPTVNLLQ
                     EMVAELFGKEEALFLPSGTMCNAIAIKVHTRMGDEMILHRDAHPYTSEGGGPAALAGI
                     SCRLLDGPRGMYTAEQVVDAISPENPHSARSRLVSIENTTNAGTGKVWPLEAIHAIAA
                     VAHERGLKTHMDGARLMNAVVASGIPAKDFVAEMDTAWVDLSKGLGCPVGGVLAGTHE
                     DMALARRYKHLYGGAMRQAGIIAAAGVYALRHNVERMAEDHANAKLLAQGLAEIPGIQ
                     IKVDDIETNIVHFDVSETGRTGAEVNEAITSRGARMGGGSGPRMRAVTHLDVSRADIE
                     RTVEIMRDAVMNA"
     gene            1297..2571
                     /locus_tag="VFV93_12695"
     CDS             1297..2571
                     /locus_tag="VFV93_12695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166251.1"
                     /translation="MRSQGVEAVGGESSPPDVVERGQRGGLLGRIPGIGNDLGLGHNN
                     LMIFWAMLLNEASFGFYQTLTPIYIESLGASPGIVGLVIGIGGLARLIFLAPGGWAAD
                     KIPLRKLIVTGRSLTIVGILLYGLAPTWWALFPAILVMSAGNIVWPSVSKVIADSTDS
                     RGRTRAFTLIYTVGPSIALLLSPSLGGLLADGVSLRSIFFAAAIAQLGSVLLFSRLRP
                     VERTSEEHGESVGFRTVLRYRPVVAVCSLFLALLLVLTTGYTLVPNFLEDEHGVGIGT
                     IGQFGSIFALGSILLGLLIAKVRHFSPPLNALILTTSMSPIAFLLLLGGGNTWVFGLA
                     FLFRGGYFVSWGLIYAILGEVSPERLRSRSFALAEVLGGFGFGIAPFIAGALYEIEPT
                     LPLVAALLAIPPLMLGILLVRRYVHGLTPATV"
     gene            2687..2896
                     /locus_tag="VFV93_12700"
     CDS             2687..2896
                     /locus_tag="VFV93_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166252.1"
                     /translation="MRLATHRLDAEILGPISGPLGTHRVEVEVIVTDRCSGDLVAVHR
                     YQVGSDFPEHEIIRHILLAARRCYD"
BASE COUNT          469 a         1124 c         1030 g          612 t
ORIGIN      
        1 ctcacgtagg ggcgatggct gcagcctcgc catttgccgt ccacacggag caggcgaggc
       61 tgcggccgtc cgttggactc acgcgttcat caccgcatca cgcatgatct cgacggtccg
      121 ttcgatgtcg gcgcgtgaca catccaggtg ggtgacggcg cgcatgcgcg ggccgctgcc
      181 accgcccatt cgggcgcctc ggctggtgat ggcctcgttg acctcagcac cagtccggcc
      241 ggtttcggag acatcgaagt gaacgatgtt ggtctcgatg tcgtcgacct tgatctgaat
      301 accgggaatc tcggccagtc cctgcgccag cagcttggcg ttggcgtggt cctcggccat
      361 ccgctcgacg ttgtggcgca gggcgtagac gccggccgcc gcgatgatgc cggcctggcg
      421 catcgcgccg ccgtagaggt gcttgtagcg gcgggccagc gccatgtcct catgcgtccc
      481 ggccaggacg ccaccgaccg ggcagccgag ccccttcgag aggtcgaccc aggcggtgtc
      541 catctcggcg acgaaatcct tggccgggat gccgctggcg acgacggcgt tcatcagccg
      601 cgcgccgtcc atgtgcgtct tcagcccgcg ctcgtgggcg acggcggcga tggcgtggat
      661 ggcctccagc ggccagactt tgccggtgcc ggcgttggtc gtgttctcga tcgagaccag
      721 ccgcgagcgg gccgagtgcg gattctcggg gctgatcgcg tcaaccacct gctcggccgt
      781 gtacatcccg cgcggcccat ccagcagccg gcaggagatc ccggccagcg cggccggccc
      841 gccaccctcg ctcgtgtacg ggtgggcgtc gcgatgcagg atcatctcgt cgcccatccg
      901 ggtgtggacc ttgatggcga tcgcgttgca catcgtcccc gacggcagga agagcgcctc
      961 ctccttgccg aacagctcgg cgaccatctc ctgcaacaga ttcacggtcg ggtcctcgcg
     1021 ccgctgctcg tcgccaacct ccgcatcggc catgaaggcc cgcatctccc gcgacggctt
     1081 cgtcgccgtg tcgctcgaca ggtcgatcag tctgtccggc atgattggtt cctccccctt
     1141 cgatcgttgt tcgggattcg ggactcggga ttcgggggta tcgagcggga cctgtcccta
     1201 caacactccc gaatcccgag tcccgaatcc cgtcccttat actacgttgc ccgcgattga
     1261 tggagcgcgc gttcgcggct gtgggagggg acacgggtgc ggtcgcaggg agtggaggcg
     1321 gttggaggag agtcctcgcc gccggatgtc gtggagcgtg ggcagcgggg tggcttgctg
     1381 ggcaggatcc ccggcatcgg caacgacctc ggcctgggcc acaacaacct gatgatcttc
     1441 tgggcgatgc tgctcaacga ggcgtcgttc ggcttctacc agacactgac cccgatctac
     1501 atcgaatcgc tcggcgccag ccccggcatc gtcgggctgg tgatcggcat cggcggcctg
     1561 gcgcggctga ttttcctcgc gcccggcggc tgggcagccg acaagatccc gctccgcaag
     1621 ctgatcgtga cgggccgctc gctgacgatc gtcggcatcc tgctctacgg gctggcgccg
     1681 acctggtggg cgctcttccc ggccatcctg gtcatgtcgg ccggcaacat cgtctggccg
     1741 tcggtctcga aggtgatcgc cgactcgacc gacagtcgcg gccggacacg cgccttcacg
     1801 ctgatctaca cggtcggacc gagcattgcg ctgctgctct cgcccagctt gggcggcctg
     1861 ctggccgacg gggtcagcct gcgctcgatc ttcttcgcgg cggccatcgc ccagctcgga
     1921 tcggtcctgc tcttctcgcg cctgcggccg gtcgagcgca ccagcgagga gcatggcgag
     1981 agcgtcggct tccgcaccgt cctgcgctac cggccggttg tcgccgtttg tagcctcttt
     2041 ctggcgctgc tgctggtgct gacgaccggc tacacgctgg tgccgaactt cctcgaagac
     2101 gagcacggcg tcgggatcgg gacgatcggc cagttcgggt cgatcttcgc gctcggcagc
     2161 atcctgctcg gcctcctgat tgccaaggtc agacacttct cgccaccgct gaacgccctg
     2221 atcctgacga cctcgatgtc gccgatcgcg ttcctgctgc tgctcggtgg cggcaacacc
     2281 tgggtctttg ggctggcgtt cctgttccgt ggcggctatt tcgtctcctg ggggctgatc
     2341 tacgccatcc tcggcgaggt ctcgcccgaa cggctccgct cgcgctcgtt cgcgctggcc
     2401 gaggttctcg gtggcttcgg cttcggcatc gcccccttca tcgccggcgc cctctacgag
     2461 atcgagccga ccctgccact cgtcgctgcc ctgctcgcca tcccaccgct gatgctcggc
     2521 attctcctcg tccgccgcta cgtgcatggg ctgacgccgg ctaccgtgtg acgcagacga
     2581 cgagccgtac acatggtaac gacgctgaac cgcgagacaa cagtttgtgc aagtctcggc
     2641 gatactatga tcgcggtcac ctcaacaggc acggatggtt tcggacatgc gccttgcaac
     2701 acatcgactg gatgcagaga tcctcgggcc gatatccggc ccgcttggaa cgcaccgagt
     2761 cgaggtcgag gtcatcgtca ccgaccgctg tagcggcgac ctggtagcgg tgcaccgcta
     2821 ccaggtcggt agcgactttc ccgagcacga gatcattcgc cacatcctgc tcgccgcccg
     2881 gcgctgctac gactgatgcc gagccgtgct ggacatgtca ggcggccgag cgagtggcgc
     2941 gcaggtcgtc gatcaactcc ttgatccaac gcacagccgc catccacagg aggcatttcg
     3001 tcgccttcgc ccgcagcatc gaccgtcatc gagctaattc aatactcgcc cgccctcagg
     3061 caagctacga ccaacccgta cggctggata ccgtgcgtga tctggccgtt tcaatcctca
     3121 ccccaccatc aggcagggtg caacggcctg ctgaccaggg cgatggtgac gcccgtcgcg
     3181 tttcaatcct caccccacca tcaggcaggg tgcaacccgc tcgacgcgca ggacc
//
LOCUS       DASUGJ010000559         3505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_229899, whole genome shotgun sequence.
ACCESSION   DASUGJ010000559 DASUGJ010000000
VERSION     DASUGJ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229899"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1128)
                     /gene="prfA"
                     /locus_tag="VFV93_12705"
     CDS             complement(49..1128)
                     /gene="prfA"
                     /locus_tag="VFV93_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX5166253.1"
                     /translation="MSTMFDRLADLDRRYDELTQLLGDPEVASNPEKLMEYGRERADL
                     DEVVSTWRELREIEQQIADAEQMARDDDPEMAELARAELDGLHDAREPIVSHLRALLV
                     PKDPLDEKNVILEIRAGTGGEEAALFAAELMRMYMRYAERQGWKTDVMDYSETGIGGI
                     KEAIIEVRGRGAYSHLRFESGVHRVQRVPVTESSGRIHTSAATVAVMPEAEDVDIQIN
                     DNDLRIDVYRSTGHGGQSVNTTDSAVRITHMPSGLVVTSQDEKSQLKNKIKAMAVLRS
                     RLFDLEQQKLDEERGGLRRSQVGSGDRSEKIRTYNFPQDRVTDHRIKTSLSNLPSIMD
                     GAIDPFIRELQAVDQAERLQMAGIG"
     gene            complement(1185..1856)
                     /gene="thpR"
                     /locus_tag="VFV93_12710"
     CDS             complement(1185..1856)
                     /gene="thpR"
                     /locus_tag="VFV93_12710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX5166254.1"
                     /translation="MATNRARRTGSTGSGQPAQRLFAAVLVSDAVREQMRAIRRELEP
                     QNWTIRWVDPDLAHLTVKFFGDTRPNQIGSLEGKLAFAAERSQPATLTTGEFLMLGPR
                     KRPNVLSLAIDAAEDDPAALDALASLAAEVDRQTAGIGAGGNERAFKAHLTLGRFKDT
                     AGAPPDVEQALAGVARTPVTFSVDRLLLVRSVLSQQGPTYTTIAEWPLSASERAETPE
                     PDDHG"
     gene            1968..2801
                     /gene="pheA"
                     /locus_tag="VFV93_12715"
     CDS             1968..2801
                     /gene="pheA"
                     /locus_tag="VFV93_12715"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX5166255.1"
                     /translation="MRIAYLGPAGTFSEEAALQYAAEGDELIPFSSFAALVSAVESDV
                     ADSAVLPIENSIEGSVSATVDLLIHETELQICREIIVPVHHCLVGAPGSKITGIKSVR
                     THPQALGQCRKFLDRCLPNANQQAALSTAGAVEDVSKGDDLTVAAIGTARSAELYGGL
                     ILARNIEDNHTNVTRFVALHERDHAQTGNDKTSLAVTVRQNVPGSLHAVLDELAADKI
                     QMTRLESRPAKNVLGEYYFLIEIEGHRLDPHIAAALERMRAKSDVFKIFGSYPKYEQA
                     D"
BASE COUNT          612 a         1141 c         1103 g          649 t
ORIGIN      
        1 gctccccgcc acccactccc gaatcccgag ccccgaatcc cgacgctctc acccaatccc
       61 cgccatctgc aatcgttcgg cctggtcgac agcctggagc tcgcggatga acgggtcgat
      121 ggcgccatcc atgatgctgg ggaggttgga gagactggtc ttgatgcggt ggtcggtgac
      181 gcggtcctgc gggaagttgt aggtgcggat cttctcgctg cggtcgccgc tgccgacctg
      241 cgagcggcgc aggccgccgc gctcttcgtc gagcttctgc tgctcgaggt cgaacaggcg
      301 cgagcggagg acggccatcg ccttgatctt gttcttcagc tgcgacttct cgtcctggct
      361 ggtgacgacc agtccggacg gcatgtgggt aatgcgaacg gccgagtcgg tcgtgttgac
      421 ggactggccg ccgtggccgg tcgagcgata aacatcgatg cgcagatcgt tgtcgttgat
      481 ctggatgtca acgtcctcgg cctccggcat aaccgccacc gtcgcggccg atgtatgaat
      541 ccggccgctc gactcggtca ccggcacgcg ctggacgcgg tggacgccgc tctcgaagcg
      601 caggtgcgaa tacgccccgc gcccacggac ctcgatgatg gcttccttga taccgccgat
      661 gccggtctcg ctgtagtcca tcacgtcggt cttccagccc tggcgctcgg cgtagcgcat
      721 gtacatccgc atcagctcgg cggcaaacag ggctgcttcc tcgccaccgg tcccagcccg
      781 gatctcgagg atgacgttct tctcgtcgag cggatccttc gggacgagca gcgcgcgcag
      841 gtgcgagacg atcggctcgc gggcatcgtg caggccatcg agctcggcgc gggccaactc
      901 ggccatctcc gggtcgtcgt cgcgcgccat ctgttcggcg tcggcgatct gctgctcgat
      961 ctcgcgcagc tcacgccagg tcgagacaac ctcgtccaga tcggcccgct cgcggccgta
     1021 ttccatcagc ttctcggggt tcgaggcgac ctcaggatcg ccaagcagct gtgtcaattc
     1081 atcgtaacgc cggtccagat cggccagccg gtcaaacatc gtcgacatgg tggagtctcc
     1141 agtgctgagt actgcgggtt gggtgctgag tggcgcgtga agcgctagcc gtggtcgtcc
     1201 ggttcagggg tctccgcgcg ctccgatgcg ctcagcggcc attcggcgat ggtcgtgtag
     1261 gttgggcctt gctggctgag gacactacga acaagcagga ggcggtcaac gctgaatgtg
     1321 acgggcgtgc gcgcgacacc ggcaagcgcc tgctcgacgt cgggcggagc accggccgtg
     1381 tccttgaagc gacccagcgt caggtgcgct ttgaaggcgc gctcgttgcc gcccgcgccg
     1441 atacctgctg tctgccgatc tacttccgcc gccaggctgg cgagcgcatc gagcgcggcc
     1501 gggtcgtcct cggccgcgtc gatcgcgagc gagaggacgt tcggccgctt gcggggacca
     1561 agcatcagga attcgccggt cgtcagcgtc gccggctgcg agcgttcggc cgcaaatgcg
     1621 agcttgccct ccagcgagcc gatctggttc gggcgggtat cgccgaagaa cttgaccgtc
     1681 aggtgagcca ggtccggatc gacccagcga atcgtccagt tctgcggctc caattcccgc
     1741 cggatcgcgc gcatctgctc gcgaacagca tcactgacca ggacggcagc gaacagccgc
     1801 tgggccggtt gtcctgatcc tgttgagccg gttcgtcgcg cacgattcgt cgccataacc
     1861 catcaagtat agacggactg gccgagtcct gtttccttct cggcattcct tcgtatactg
     1921 ccacaacaga aatcgacggc aggaacgtgg gaagggtcga gggatccatg cgcatcgcct
     1981 atctgggacc ggcagggaca ttcagcgagg aagcggcgtt gcagtacgct gccgagggcg
     2041 acgagctgat cccgttttcc agctttgccg cgctggtctc agccgttgag tccgatgtcg
     2101 ccgacagcgc cgtcctgccg atcgagaact cgatcgaagg ctcggtctcg gcgacggtcg
     2161 acctgctgat ccacgagacc gagctgcaga tctgccgtga gatcatcgtc ccggtccatc
     2221 actgcctggt cggcgcgccc ggctcgaaga tcaccggcat caagagcgtc cgcacccacc
     2281 cgcaggcgct ggggcagtgc cgcaagttcc tcgaccgctg cctgccgaac gccaaccagc
     2341 aggcggccct ctcaacggcc ggcgccgtcg aagatgtctc gaagggcgac gatctgacgg
     2401 tggccgcgat cggcacggct cgctcggcgg agctgtacgg cgggctgatc ctggctcgca
     2461 acatcgagga taaccacacc aacgtcaccc gcttcgtcgc gctccacgag cgggatcacg
     2521 cccagaccgg caatgacaag acctcgttgg ccgtcaccgt ccgccagaac gtgcccggct
     2581 cactgcacgc cgtcctcgac gagctggccg ccgacaagat ccagatgacg cggctggaga
     2641 gccgcccggc caagaacgtg ctcggcgagt actacttcct gatcgagatc gaaggccacc
     2701 gcctcgaccc ccacatcgcc gccgccctcg aacggatgcg cgccaagtcg gatgtcttca
     2761 aaatcttcgg ctcatatcca aagtacgaac aggcggactg atttcacccg tggttgtgag
     2821 ggcttgtgtc gaaggcgact gagtgggacg aaatccgcgt caacggtagg tatcaacttg
     2881 ccatggcgta tggtccgacg attacgttct ttcagcgcga tcgatccaac gactgatcaa
     2941 ggagcgcaag cccgcaagac gtgatcgtaa cccgatcgtc ctgtggacca gaaccgcgct
     3001 caacgatgac cagtccatcg gcgagtgcgt cctcccagat cgacaggcgc ggacaactgg
     3061 tacgccacgc gtccatcacc tcctcgtacg agcggggttg ctcggctacc cagcggagga
     3121 agtccaaggt caacgcgtgc gctggcgcgt acgtggtgcg ctgatctgcg atgctcatat
     3181 ccctctcctc gcagtcgaga acgagcgacg ttacgtgtgc aacgatagcc ttggaatgag
     3241 ccgaaaatcg cgaacgctga gcgtcagaag cgtctggccg tcgtcctcga gttggtcgag
     3301 cgtatcgatc gcccgttggt ccctgtggag gatctgaatg attcagtcgg tatcaacgag
     3361 atagagactc atagcttgac ggacggcctg tttgatgtgc gacgacgggc atagatgtat
     3421 gccgtgaact cggccgcatc caccaggcct ttccaaccac ctgctgcctt ccgagttcct
     3481 gatctcgaac gatcgacctg ctcac
//
LOCUS       DASUGJ010000560         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1478787, whole genome shotgun sequence.
ACCESSION   DASUGJ010000560 DASUGJ010000000
VERSION     DASUGJ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478787"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1300)
                     /locus_tag="VFV93_12720"
     CDS             complement(53..1300)
                     /locus_tag="VFV93_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166256.1"
                     /translation="MGRRFIPTIIVMAVAAFAVAAVALAFVADEPRLWSAAVALIVLG
                     GITPMIYAVNVRIVPVFSRRQWVNPTQVYAAISAGVAGGWLVFLGRWLDEAWVERAGH
                     GLALIGGALFVASLIRLFTSEPVTKVAPPLPFPEQAAIDKIGISFTRMAGMYLLLGLA
                     VGLMMTFWTPDRGRWELVWAHAMLLGWFLQMVAGVCYHVLSRWTGARWQSPQRIRWHL
                     LTVTLGLPAMVAALALDLDWLFAVAGPLQAIALGLFVWNIAPLVLRMPSLSRLGMLLA
                     AGFLVVGITIGASAALDPANGIRLRFSHATINLLGFAGLLICGVGYYLFPRLAGQALR
                     WPRLATIQIAAHASGVALVAAAWWYQLAVDDGAQPLITAGALLVAAGFAVFGGIVAAT
                     FQGSGRGVVAGVSLQPRRSPQGR"
     gene            complement(1302..1535)
                     /locus_tag="VFV93_12725"
     CDS             complement(1302..1535)
                     /locus_tag="VFV93_12725"
                     /inference="COORDINATES: protein motif:HMM:NF016303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HEX5166257.1"
                     /translation="MSQQSTIEQQIATMIETEPIRRVVELHPEVMPILAGHGMDLCCG
                     GGHTIQEAAQLHGLDAELLAEQVAAAIAVRARG"
     gene            complement(1659..>2192)
                     /locus_tag="VFV93_12730"
     CDS             complement(1659..>2192)
                     /locus_tag="VFV93_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166258.1"
                     /translation="ISSGMYGLIIVEPEGGLPKVDREFYMMQGDFYLEGARGEQGLRA
                     FSMDKMLDERPDYVVFNGAVGAIAGDRAFKANVGETVRIFFGVGGPNILSSFHVIGEI
                     FDRVMLEGATEWSTNVQTTLVPAGGATIVDFKVDVPGNLTIVDHSLGRLAKGAAATIV
                     VEGDENPEVFEIVEAAH"
BASE COUNT          393 a          782 c          702 g          315 t
ORIGIN      
        1 ccagagcagc gacgcgcgca ccagccggac gctgacaggg tccatctcgc cgctatcgtc
       61 cctgcgggct gcggcgcggc tggaggctga caccggcgac gacgccccgg cccgagccct
      121 ggaaggtcgc ggcgacgatc ccgccgaaga cggcgaagcc ggccgccacc agcaacgcgc
      181 cggccgtgat cagtggctgc gcgccgtcat cgactgccaa ctgataccac caggccgccg
      241 ccaccagcgc gacgccgctc gcgtgggctg cgatctggat cgtcgccagc cggggccagc
      301 gtagtgcctg accggccagt cgcgggaaga ggtagtagcc gacgccgcag attagcaggc
      361 cggcgaagcc cagcaggttg atcgtcgcgt ggctgaagcg cagccggatg ccgttggcgg
      421 gatccagcgc cgccgacgcc ccgatcgtga tcccgacgac gaggaaaccg gccgccagca
      481 acatccccag ccgcgagagg gacggcatcc tgaggaccag cggcgcgatg ttccagacga
      541 acaggccgag cgcgatggcc tgcaacgggc cggcgactgc gaagagccag tcgaggtcga
      601 gcgccagcgc ggccaccatc gccggcaggc caagcgtgac ggtcagcagg tgccagcgga
      661 tgcgctgtgg cgactgccag cgcgcgccgg tccagcgcga gaggacgtgg tagcagacgc
      721 cagcgaccat ctgcaggaac cagcccagca acatcgcgtg cgcccagacg agctcccagc
      781 gaccccggtc gggcgtccag aacgtcatca tcagcccgac ggcaaggccc agcagcagat
      841 acatcccggc catccgcgtg aagctgatgc cgattttgtc gatggccgcc tgctccggga
      901 agggcagcgg cggggcgacc ttcgtcaccg gctccgacgt gaagaggcgg atcaggctgg
      961 cgacgaacag ggcgccgccg atcagcgcca ggccgtgacc ggcccgctcg acccacgctt
     1021 cgtccagcca gcggccaaga aagacgagcc agccgccggc gacaccggcc gagatggcgg
     1081 cgtagacctg tgtcgggttg acccactggc gccggctgaa gaccggcacg atccggacgt
     1141 tgacggcgta gatcatcggc gtgatcccgc cgaggacgat cagcgcgaca gccgcactcc
     1201 acaggcgcgg ctcatcggcg acgaacgcta gcgcgacggc ggccacggca aatgccgcga
     1261 ccgccatcac gatgatcgtt ggaataaagc ggcgtcccat ctcaacccct cgcgcggacc
     1321 gcgatcgccg ccgcgacctg ctcggcgagc aattccgcgt ccagcccgtg caactgggcg
     1381 gcttcctgga tcgtatgccc gccgccgcag cacaggtcca ttccgtgccc ggccaggatc
     1441 ggcatcactt ccgggtggag ctcgacgacc cggcggatcg gctcggtctc gatcatcgtt
     1501 gcgatttgct gctcgattgt tgactgctgg ctcatgctgt tctcacatcc ctctctgatg
     1561 ataccgctgt tcgtgggaag cggagccaga ctcgcgcctg actccgcccc ttcccccgtc
     1621 ctcccggccc ccctgcctcg ggccgggccg atgatgttct agtgcgccgc ctcgacgatt
     1681 tcgaagacct ccggattctc gtcgccttcg acaacgatcg tcgcagctgc gcccttggcg
     1741 aggcggccca ggctatggtc aacgatcgtc agattaccag ggacatcgac cttgaagtca
     1801 acgattgtcg cgccgccggc cgggaccagt gtcgtctgga cgttggtcga ccattcagtc
     1861 gcgccctcca gcatgacccg gtcgaagatt tcgccgatga cgtggaagct cgacaggata
     1921 ttcggtccac cgaccccgaa gaatatccgg acggtctcgc ccacgttcgc cttgaaggca
     1981 cgatccccgg caatcgcgcc gaccgcgccg ttgaagacca cgtagtcggg ccgctcatcg
     2041 agcatcttgt ccatcgagaa ggcccgcaac ccctgctcgc cccgtgcacc ttccaggtag
     2101 aagtcgccct gcatcatgta gaactcgcga tcgaccttcg gcagaccacc ctctggctcg
     2161 acgatgatca ggccgtacat gccgctcgag at
//
LOCUS       DASUGJ010000561         5520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_924328, whole genome shotgun sequence.
ACCESSION   DASUGJ010000561 DASUGJ010000000
VERSION     DASUGJ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924328"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFV93_12735"
     CDS             complement(<1..1080)
                     /locus_tag="VFV93_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEX5166259.1"
                     /translation="MRLNAILTWTVKKVMMKSETREFLGRLLETASPSGFEVEAARVW
                     RAEAETFADEVSVDVNGTSYARLKGSGPKVMIEGHIDEIGVMVTHIDDNGFLWFSAIG
                     GWDDQVLVGQRIRVLAESGPIVGVIGKKPRHQMTEEDMNKVSKIRTLWIDIGAKDGDD
                     ARSRVAIGDPGVIEQPLIELGDDLIACRGLDNRVGAFVALEALRYLSAGERPAADVWA
                     VATNQEEITFGGARTGTFGLDPDVAIAIDVTHATDHPDADVRGNGLCKIGGGPTLARG
                     SAVHPGVNRRLVEAARDEGIPHILEATPRSTGTDADAIAYARAGVPTGIVSIPNRYMH
                     SPSEIVSLTDLDNCSKLIAAFTRRLG"
     gene            complement(1320..2936)
                     /locus_tag="VFV93_12740"
     CDS             complement(1320..2936)
                     /locus_tag="VFV93_12740"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5166260.1"
                     /translation="MSDITIDDLLHWERGLSFQPPAGMATDEGLARVVSWAAAIRVTA
                     PIVPALRGGEIIIAPPRTLERIEQSEGLDRAGFLRMLADQPIAALMVDRAFVDDGPAE
                     IGVPLLITSGAFPADAESVLNRLFTERRADLYGIGSALSRALSSATMSGAGLDALLDA
                     ASVAGGHALVLLDADGAIVAQSSEAPDAVSLPVELAARRGGRREGGIWRVDLDGRSWL
                     VQALGNAARTAGTPGLALAIELEPMASTEIQRLTLAQTGAAVELMLGQSGPDGVSARD
                     RWNREALISDLLLGRLASREAADARARLLGVDPTQPARLALFVTQRPTALSRLRSALA
                     DERGRVAAAIGEGEYAVLLTGAPATAHEYRELSSALRTLAAQDPEAILVLSELLAGAS
                     RAPAALDQARLLVRLERDGAISGPLIRADAYDTVGAFGLLLPFVSGPDIDIVEARERM
                     AAFAGTLLGPLEEHDARRGSDLVATLEAYLQLGGALAQAAERLNIHRNTLSYRLGRIG
                     ELTGRDLNDPATRFLMQAALIVRAFERAAG"
     gene            3228..4892
                     /locus_tag="VFV93_12745"
     CDS             3228..4892
                     /locus_tag="VFV93_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5166261.1"
                     /translation="MDRLTAMHVVIERENPTPIYLQIAQQLRQSILDGEPPAGSRLPP
                     ERRLANLLGVNRTTIVNAYRELAADGLVSGQVGRGTIVIYGAGDAAEAVDVTSWPRVN
                     GHVAPEVTSSLSNGDHFDAAHRSRNGSVPWAQLFTAVTDVMDDPLLHDAMTVSARPDV
                     IRFATGTPSPELYPIDAIRSIFDEALHNAGSTLLQHTPTEGYPPLREQLSAWMERRAA
                     AAGAKVHVEPNQVVVVAGSQQGLYLLARTLIEPGDLVAVESPTYIGAAHVFRAAGARL
                     LPIPVDGDGMQVDLLQDLLPRRRPKLIYTLPTFQNPSGSVMAMGRRLRLLDLAARYQI
                     PIIEDDPCGALRYEGRALPTLATIDRAEGRGNVIYLSTVSKMLFPGFRIGWISAVKPV
                     IEQVTQMKQLVDLDTNALAQRAVCAFFERGLLDEHLTRVNRVYPQRRDRMLDALQRNA
                     GDLISANRPDGGIYLWCRINEGIRVRDLLPEAAREGVVFAPGESFHVDAASGRGRFDI
                     RLNFTLPNDAAIDEGVRRLGVALHRLAEHLDPAAHAHPARPLTPIV"
     gene            4956..>5520
                     /locus_tag="VFV93_12750"
     CDS             4956..>5520
                     /locus_tag="VFV93_12750"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX5166262.1"
                     /translation="MLDEPNGQPTEHESEPGREIQPPLFDVTSPGAFAPRISGVAALT
                     PTSPLPVARYWFRRYLEQSQHPRNTIESYLYDLSIFEQVVGLKTIDSVTARDIARYLG
                     EANTRSTRKRRLTSVSQLFKWLTNGARMLDRDPTESFYPDHIPLKTPRPLFDAEQRAV
                     LDAAANDSSRSAIMIWLMLRLGVSRGEL"
BASE COUNT          890 a         1979 c         1778 g          873 t
ORIGIN      
        1 gcccagccgg cgcgtgaagg cggcgatcag cttcgagcag ttgtcgagat cggtcaggct
       61 gacgatctcg ctcggtgagt gcatgtagcg gttcgggatc gagacgatcc cggtcgggac
      121 accggcccgg gcataggcga tcgcgtcggc gtcggtgccg gtcgagcgcg gagtcgcttc
      181 gaggatgtgc gggatgccct cgtcgcgggc ggcctcgacc agccggcggt tgacgccggg
      241 gtggacggcc gagccgcgcg ccagcgtcgg tccgccgccg atcttacaga ggccgttgcc
      301 acgcacgtcg gcgtccggat gatcggttgc gtgcgtcacg tcgatggcga tcgcgacatc
      361 cgggtcgagt ccgaaggtgc cagtccgcgc gccgccgaag gtgatctcct cctgattggt
      421 cgcgactgcc cagacatccg ccgccggacg ctctcctgcc gacaggtagc gcagtgcctc
      481 cagcgcgacg aaggcgccga cgcggttatc cagcccgcga caggcgatca ggtcgtcgcc
      541 cagctcgatc agcggctgct cgatgacgcc cggatcgcca atcgcgaccc tcgatcgggc
      601 gtcgtcgccg tccttcgcgc cgatgtcgat ccagagtgtc cggatctttg agaccttatt
      661 catgtcctct tcggtcatct ggtggcgcgg cttcttgccg atgacgccga cgatcgggcc
      721 ggactcggcc aggacgcgaa tccgctggcc gaccaggacc tgatcgtccc agccaccgat
      781 cgcgctgaac cagaggaagc cgttgtcgtc gatgtgagtc accatcacac cgatctcgtc
      841 gatgtgaccc tcgatcatca cctttgggcc gctgcccttc aggcgcgcgt acgacgtacc
      901 gttcacatcg acgctgacct cgtcggcgaa cgtctccgcc tcggcccgcc agacgcgggc
      961 cgcctcaacc tcgaaccccg acgggctggc cgtctccagc aaccgcccca ggaactccct
     1021 cgtctcgctc ttcatcatca ccttcttcac cgtccaggtc agaatcgcgt tcaaccgcac
     1081 gaacgacgat cgtaccatcg tagtcggcag gacgcgggaa cctccccaga catccgatat
     1141 tcttcagaat ccgaccattg tctccctgtc gtcctaaggc cgcagccgaa ggacctctcc
     1201 gcgtcaacca ctcatgacgc cacgtaagga gaaatccttc ggctgcggcc tcaggacgac
     1261 aaggagactg cgggcctcga acattgggaa actgcttccc ggccctcaac acagcgccat
     1321 tacccggccg cccgctcaaa cgcgcggacg atcagggcgg cctgcatcag gaagcgggtg
     1381 gcggggtcgt tcaggtcgcg gccggtcagt tcgccgatcc ggccgaggcg gtaggagagc
     1441 gtgttgcgat ggatgttgag ccgctcggca gcctgtgcca atgcgccgcc gagttgcaga
     1501 taggcttcaa gggttgcgac cagatcactt ccgcgccgcg cgtcatgctc ctccagcgga
     1561 cccagcagcg tcccggcgaa ggcggccatc cgctcgcggg cctcgacgat gtcgatgtcg
     1621 gggcccgaga cgaacggcag cagcaggccg aatgcgccga ccgtatcgta ggcgtcggcg
     1681 cgaatcagtg ggccgctgat cgcgccgtcg cgctcgagcc gaaccagcag ccgcgcctga
     1741 tcgagcgcgg ccggcgcgcg cgaggctccg gccagcagtt cgctgaggac gaggatcgcc
     1801 tccgggtcct gggcggccag cgttcgcagc gcggaactca actcacggta ctcgtgggcc
     1861 gtcgccggtg cgccggtcag caggacggcg tattcgcctt cgccgatcgc ggcagcaacc
     1921 cggccccgct catcggccag tgccgagcgc aggcgcgaga gcgcggtcgg ccgctgggtg
     1981 acgaacagcg ccagccgggc cggttgggtc ggatcgacgc caagcagcct ggcgcgggcg
     2041 tcggcggcct cgcgtgacgc cagccggccg agcagcaggt cggagatcag cgcctcgcgg
     2101 ttccaccggt cacgggcgct gacgccatct ggccccgact gacccagcat cagctcaacg
     2161 gcagcaccag tctgggccag cgtcaaccgc tggatctcgg tactggccat cggctccaac
     2221 tcgatcgcca gcgccaggcc gggtgtgccg gctgtccgcg cggcattgcc gagcgcctgc
     2281 accagccacg agcgtccgtc gagatcgacc cgccagattc cgccttcccg ccgtccgcca
     2341 cgccgcgccg ccagttcaac cggcaacgag accgcatcgg gagcctccga tgactgggcg
     2401 acgatcgccc catccgcgtc cagcagcacc agcgcgtgcc cgccggcgac cgacgccgcg
     2461 tccagcagcg catcgaggcc ggcgcctgac atcgtcgccg acgacagcgc gcgggacagc
     2521 gccgagccga tgccgtacag atcggcccgc cgctcggtaa agagccggtt gaggaccgac
     2581 tcggcgtcgg ccgggaatgc gccactggtg atcagcaacg gtacgccgat ctcggccggg
     2641 ccgtcatcga cgaaggcccg atcgaccatc agggccgcga tcggctggtc ggccagcatc
     2701 cgcaggaacc cggcccggtc cagcccttcg gactgctcga tccgctccag cgtccttggc
     2761 ggtgcgatga tgatctcgcc gccgcgcagg gccggcacga tcggcgccgt cacgcggatc
     2821 gccgccgccc agctgacgac tcgtgccagc ccctcgtccg tcgccattcc ggccggcggc
     2881 tggaagctca gcccccgttc ccaatgcagt agatcgtcga tcgtgatgtc cgacattctc
     2941 ccgcttcctg ccaagtcaag tccccgccgt tttgtcatcc tgaggcgtga gccgaaggat
     3001 ctctcttcgg ctcacgcctc aggatgacaa gggcagtggc gcaccactcg gtcgcgctgc
     3061 acaatgactt tccaccattg tgaatgatac gcgcttgatc ggcggcgact gttgtgcgtg
     3121 gtgcatatgc cacatgtaat cctctcggca tgccgttacc ggcccggtcg tgcaacaatc
     3181 ggggtattgg cgtacgccaa ttgtcgtgcg agccatcgat gccgggagtg gacaggctga
     3241 ctgcaatgca tgtcgtcatc gaacgcgaga atccgacgcc gatctacctc cagatcgccc
     3301 agcagttgcg gcagtccata ttggatggcg aaccaccggc cggctcgcgc ttaccgcccg
     3361 agcgtcgatt ggccaacctg ctcggcgtga accgaacgac gatcgtcaac gcctaccgcg
     3421 agttggcggc cgatggcctc gtctccggtc aggtcgggcg cgggacgatc gtgatctacg
     3481 gcgcaggtga cgcggccgaa gccgttgacg tcaccagctg gccgcgcgtt aacggacacg
     3541 tggcgccgga ggtgacgtcc tcgctatcga atggcgacca cttcgacgcg gcccaccgct
     3601 cgcgaaatgg ttccgtcccc tgggcacagc tctttacggc cgtgactgac gtcatggacg
     3661 acccgctgct ccacgacgcg atgaccgtct cagccaggcc ggacgtgatc cgcttcgcga
     3721 ccgggacgcc atcgccggaa ctgtacccga tcgacgcgat ccgctcgatc ttcgacgaag
     3781 cgctccacaa cgccggctcg acgctgctgc aacacacgcc gaccgagggc tacccgcccc
     3841 tgcgcgaaca gctctcggcc tggatggagc gccgcgcagc ggcggccggg gcgaaggtcc
     3901 atgtcgagcc gaaccaggtc gtcgtcgtcg ccggatcgca gcaggggctt tacctgctgg
     3961 ccaggacgct gatcgagccg ggcgacctgg ttgccgtcga gtcgccgacc tacatcggcg
     4021 cggcccatgt cttccgggcg gccggcgcgc ggctgctacc gatcccggtc gacggcgacg
     4081 gcatgcaggt cgatctgttg caggacctgt tgccgcgccg ccgccccaag ctgatctaca
     4141 cgctgccgac cttccagaat cccagcggct cggtgatggc gatgggccgt cgcctgcgcc
     4201 tgctcgacct ggcggcccgc taccagatcc cgatcatcga ggacgatccg tgcggcgcgc
     4261 tgcgctacga gggccgggcg ctgccgacgc tggcgacgat cgaccgggct gagggtcgcg
     4321 gcaacgtcat ctatctcagc accgtctcga agatgctctt ccccggcttc cggatcggct
     4381 ggatctcggc cgtcaagccg gttatcgaac aggtgaccca gatgaagcag ctggtcgacc
     4441 tggacactaa cgcgctggcc cagcgggccg tctgcgcctt cttcgagcgc ggcctgctcg
     4501 acgagcacct cacccgcgtc aatcgcgtct acccgcaacg gcgcgaccgg atgctcgacg
     4561 cactccagcg caacgccggc gacctgatca gcgccaaccg gccggacggc ggcatctacc
     4621 tctggtgccg gatcaacgag ggcatccgcg tccgcgacct gttgcctgaa gcggcccgcg
     4681 aaggggtcgt cttcgccccc ggcgagtcgt tccacgtcga cgccgcgtcg ggtcgtggcc
     4741 ggttcgacat tcggctcaac ttcacgctgc cgaatgacgc cgcgatcgac gagggggtcc
     4801 gccggctggg cgtcgccctc caccggctgg ccgagcatct cgatccggcc gcccacgccc
     4861 acccggcccg gccgctgaca ccgatcgtct gatggttgac actagaacgg atgttccgct
     4921 agagtacgcc gaacggcgac tgccagggag cgcgcatgct ggacgagccg aacggacagc
     4981 ccacggaaca cgagagcgag ccgggccggg agatccagcc gccgctgttc gacgtgacca
     5041 gcccgggcgc cttcgcgcca cgcatcagcg gcgtcgcggc cctgacgccg acctcgccgc
     5101 tgccggtcgc gcgctactgg ttccggcgct acctcgagca gtcgcagcac ccacgtaaca
     5161 cgatcgagtc gtacctctac gacttgtcga tcttcgagca ggtggtcggc ctgaagacga
     5221 tcgacagcgt cactgcccgc gacatcgccc gttacctggg cgaggccaac acccgctcga
     5281 cccgcaagcg gcggctgacc tccgtctcgc agctgttcaa gtggctgacg aacggggcca
     5341 ggatgctcga tcgcgacccg accgagtcgt tctatcccga ccacatcccg ctgaagacgc
     5401 cccgcccact cttcgacgcc gagcaacggg ctgtcctcga cgcggccgcc aacgattcga
     5461 gccgctcggc gatcatgatc tggctgatgc tgcggttggg cgtctcgcgc ggcgagctgt
//
LOCUS       DASUGJ010000562         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1062986, whole genome shotgun sequence.
ACCESSION   DASUGJ010000562 DASUGJ010000000
VERSION     DASUGJ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062986"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFV93_12755"
     CDS             complement(<1..231)
                     /locus_tag="VFV93_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166263.1"
                     /translation="MNARTATPMQRKPGQRMVRTRFFSARVNVHVLLFAVIALLVLAI
                     LATWAMTLGSYRIPFVDVAKAVTGTGADDQVFI"
     gene            complement(243..1304)
                     /locus_tag="VFV93_12760"
     CDS             complement(243..1304)
                     /locus_tag="VFV93_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5166264.1"
                     /translation="MTTSDASQAFQQAQQRERLGGTGLALRGIGLLIGLAALAVVAIL
                     SLRIGSIDISTSDAWNALFHYDSEVYNQTVVRTLRLPRTVIALGVGAALAVAGATMQA
                     VTRNPLADPSILGVSSGATFAIVTATYYLRLANPSEYIWFAFAGALGASLLVFAIGSA
                     GREGPSPIKLALAGVVISALLGSWTSALLLLDESTLDTVRFWLAGSVVGRDLDTFWFL
                     SPFMLGGTAAMIFLGHQLNVLNLGEDSARALGMHTGRMRLICSLLVVVVVGAAVSAAG
                     PIAFVGLATPHIVRAMIGPDYRWVLPYSAITGALLLTAADITGRIVARPAEIQVGIMT
                     AVVGAPFLIYLARQRVVEG"
     gene            1638..>2168
                     /locus_tag="VFV93_12765"
     CDS             1638..>2168
                     /locus_tag="VFV93_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166265.1"
                     /translation="MLTYRVSRRRLLAAVPGGLLLAACGGDDDDDDEPATTAAGGAST
                     EASSATSASDATPETASTPSAATGSPSAGDTGELRLIPLPPAELGAPEVPEPAVSTAE
                     GAVPVAEAVAVYDDFGTDAEPGVFPRTIRHAMGETTLDAKPERVVVLDSGELDSVIRM
                     GLNPVGALEYDPDLLPD"
BASE COUNT          378 a          710 c          765 g          315 t
ORIGIN      
        1 gatgaagacc tgatcgtcag cgccggttcc ggtcacggcc ttggcgacat cgacgaacgg
       61 gattcggtag ctaccgagcg tcatcgccca ggtcgccaga atcgccagaa cgagcagcgc
      121 gatgacggcg aagagcagga cgtggacgtt gacgcgcgcc gagaagaaac gcgtcctgac
      181 catgcgctgg ccgggcttgc gctgcatcgg cgtggcggtg cgtgcgttca tcccgctcat
      241 ccctagccct ccaccacgcg ctggcgggcg aggtagatca ggaacggcgc gccgacgacg
      301 gccgtcatga tgccgacctg gatctcggcc ggccgggcga cgatccggcc ggtgatgtcg
      361 gccgccgtca gcagcagcgc gccggtgatg gccgaatagg gcagcaccca gcggtaatcc
      421 ggcccgatca tagcccggac gatgtgcggc gtcgccagtc cgacgaaggc gatcggcccg
      481 gccgccgaga cggccgcgcc aacgacgacg accaccagca gcgagcagat cagccgcatc
      541 cggccggtgt gcatccccag cgcgcgggcg gaatcttcgc cgaggttgag cacgttgagc
      601 tgatggccga ggaagatcat cgcggccgtc ccgccgagca tgaacggcga caggaaccag
      661 aacgtatcca gatcgcggcc gacgaccgag ccggccagcc agaagcgaac cgtgtcgagc
      721 gtgctctcat cgagcagcag cagcgcactg gtccacgagc cgagcagggc cgagatgacg
      781 acgccggcca gcgccagctt gatcggcgac ggcccttccc gcccggccga gccgatcgcg
      841 aagaccagca gcgaagcgcc gagcgcgccg gcaaacgcga accagatgta ctccgacggg
      901 ttcgccagcc gcaggtagta ggtggccgtc acgatcgcga aggtcgcgcc gctgctgacg
      961 ccgaggatcg acggatcggc cagcgggttg cgggtgacgg cctgcatcgt cgcgccggcc
     1021 accgctagcg ccgccccgac gccgagtgcg atgaccgtcc gtggcagccg cagcgtccgg
     1081 acgacggtct ggttgtagac ctcgctgtcg taatggaaga gcgcgttcca ggcgtcactg
     1141 gtgctgatgt cgatcgagcc gatccgcagg ctgaggatcg cgacgaccgc cagcgcggcc
     1201 aggccgatca gcagcccgat cccgcgcagc gccagcccgg ttccgcccag ccgctcgcgc
     1261 tgctgggcct gctgaaacgc ctgtgatgcg tcactcgtcg tcattacacg gtccaagctt
     1321 ccgactggcc gctatcaggg aggtggagtg cggcgacgtt ggtggctccg gcggttgcgt
     1381 tcagcctgct ttgaacgctc gggagattcg ttcccagcga atcccgactc ttgcagtcgg
     1441 agtttatgat aatggtctgc ggcaggttct ggcaaggcgg aaagcgcaat tttcgactgt
     1501 tcagccggcc ttgacaggct ggcggcggat tgcgttactt aaaacccgac taaaacagtc
     1561 gggtttctgc tgtctggcgc gtgggaggac aacgaacagg gatggtcatc gatcgtaatg
     1621 aggatcggac gcaccggttg ctgacgtacc gagtcagccg tcgcaggctg ctggcggcag
     1681 tccccggtgg cctgctgctc gccgcttgcg gtggcgatga cgatgacgac gacgagccgg
     1741 cgacgacggc ggccggcggg gcatcgaccg aagcgtcgag cgcgaccagc gcaagtgatg
     1801 cgacacccga gacggccagc acaccgtctg cggcgaccgg atcaccgagc gcgggcgaca
     1861 ccggcgagtt gcggctgatc ccgctgccgc cggccgagct tggcgcgccg gaggtgccgg
     1921 agccggctgt ctcgacggcc gagggcgctg tcccggtcgc cgaagcggtt gccgtctatg
     1981 acgacttcgg caccgacgcc gagccgggcg tcttcccgcg cacgatccgc cacgcgatgg
     2041 gcgagacgac gctggacgcc aagccggagc gggtggttgt actggatagc ggcgagctgg
     2101 attcggtcat ccggatgggc ctcaacccgg tcggcgcgct ggagtacgac ccggatctgc
     2161 tgcctgac
//
LOCUS       DASUGJ010000563         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_89773, whole genome shotgun sequence.
ACCESSION   DASUGJ010000563 DASUGJ010000000
VERSION     DASUGJ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89773"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFV93_12770"
     CDS             <1..228
                     /locus_tag="VFV93_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166266.1"
                     /translation="DANRNVNIAGRLVAGRDRTVVWSRRADAGYTVAIGRLDSPWLYV
                     ITASNERDLEAALRALTGAAGTHGGRPATLR"
     gene            336..743
                     /locus_tag="VFV93_12775"
     CDS             336..743
                     /locus_tag="VFV93_12775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5166267.1"
                     /translation="MKSSLSHMQFNVQAENVPFYKDLFSFLGWKTEHEDTGVIGVSGT
                     DGASLWFIGVAKDVSNDYDGPGMNHLAIGAESQADVDAAAKHLTGNGVEMLFNTPCHR
                     PEFSGEGTTYYQIMFETPDRILLEFVYAGPKQD"
     gene            complement(811..1620)
                     /locus_tag="VFV93_12780"
     CDS             complement(811..1620)
                     /locus_tag="VFV93_12780"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5166268.1"
                     /translation="MSDVWSMVPELDDAVQERMAAVLETRGADPQQQAMRRVFLSDIA
                     FPDGARVLDVGCGTGVLTRVIAGWPGVAEVVGVDPAPSLLEKARGLAAGLTNLTFEVA
                     DGRELPYEDDSFDVVIFDSTLCHVPHPERALAEAFRVLRPSGWLAAFDGDYATTTVAL
                     GDHDPLQACADVTMANSVNDRWLVRRLPALARACGYEVVGFRSHGFTETTEGGYMLTV
                     VERGADMLHAFGQISGETAAMLKAEAYRRVEGKTFFGHIAYGSLTARKPGA"
     gene            complement(1770..2459)
                     /locus_tag="VFV93_12785"
     CDS             complement(1770..2459)
                     /locus_tag="VFV93_12785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5166269.1"
                     /translation="MISYPSTFEMPTTDPLTGLLSPAYFRHLLREDLIRQAQEAGDPL
                     SVFLMDTDSFHELNVSHGRQAGDAILLGVSRILRETLPESAVIVRYGGDEFGGALPDT
                     RLDDAFTLVEELRRRIGAMRLDEWPDLAITCSIGLAVYPANGATDIELVREADQALYL
                     AKATGRNKVSLPLADDRMITKTSYYTRTQLERLAALAKTLGRNEASLLREALDDLLKK
                     YNDQLGAPPRE"
BASE COUNT          504 a          838 c          827 g          456 t
ORIGIN      
        1 gacgccaacc ggaacgtgaa catcgccgga aggctggttg ctggccgcga ccggaccgtc
       61 gtctggtcgc gccgcgctga tgccggctac acggtcgcca tcggccggct ggatagcccg
      121 tggctctatg tcatcacggc gtcgaatgaa cgcgatcttg aggccgcgct gcgggccctg
      181 accggcgcgg ccggaacgca tggtggacgc cccgcgacgc tccgctgagg tctggacaaa
      241 agtagaacag atgctctatt ctacacatcc ccagcgcgac gacgtgcctc ggggagatgt
      301 ccacggcgga tcgtagagag acaaggggaa ccagcgtgaa gagcagcttg tcacacatgc
      361 agttcaacgt ccaggccgag aacgtcccgt tctacaagga cttgttctcg ttcctcggct
      421 ggaagaccga acacgaggac accggcgtca ttggcgtctc cggaacggac ggtgcaagcc
      481 tctggttcat cggtgtggcg aaggatgtca gcaacgacta cgacgggccg gggatgaacc
      541 acctcgcaat cggcgccgaa tcgcaggccg acgtcgacgc ggcggcgaag cacctgaccg
      601 gtaacggcgt cgagatgctc ttcaacaccc catgccaccg cccggagttc tccggcgagg
      661 gcaccaccta ctaccagatc atgttcgaaa cccccgaccg catcctgctg gagttcgtct
      721 acgcggggcc gaagcaggac tgaagaccgg gaccgggacg agggacgcgg gacgcgcaac
      781 accgactccg cgtcccacgt cgctccattt ttatgcaccg ggcttgcggg ctgtcaggct
      841 gccgtaggcg atgtggccaa agaaggtctt gccctcgacc cggcggtagg cttcggcttt
      901 gagcatcgca gccgtctcgc cgctgatctg gccgaatgca tgcagcatgt cagcgccacg
      961 ttcgacgacc gtcagcatgt aaccaccctc ggtcgtctcg gtgaagccgt ggctgcgaaa
     1021 gccgacgacc tcatagccgc aggcacgagc gagtgccggt agacggcgca ccagccagcg
     1081 atcgttgacc gagttggcca tcgtcacatc cgcgcaggct tgcagcgggt cgtggtcgcc
     1141 aagcgcgacc gtcgtcgtcg cgtagtcgcc atcgaacgct gccaaccagc ctgacggccg
     1201 caggacgcgg aacgcctcgg cgagagcgcg ctcagggtgc ggcacgtgac agagcgtcga
     1261 gtcgaagatg acgacgtcga agctgtcgtc ttcgtatggc aactcccggc catcagccac
     1321 ctcgaaggta agattggtca gcccggcagc caacccgcgg gccttctcga gcagcgacgg
     1381 ggccggatcg acgccgacga cctcggcaac gccgggccaa ccggcgataa cccgcgtcag
     1441 aacaccggtc ccgcagccaa cgtccagcac ccgcgcccca tcgggaaagg cgatatcgga
     1501 caggaagacg cggcgcatcg cctgctgctg cgggtccgcg ccacgagtct cgaggacagc
     1561 cgccatccgc tcctggacgg catcatccag ttcaggaacc atcgaccaca catctgacat
     1621 gggttcctcc tctctccggc ggggtcaacc gaccggattg gcagatatcg cgcagtacac
     1681 gagtgcattg ctgcgattgt cccaattgtg atgatcagat attactcgaa tgttgcgtca
     1741 taaccgcgta tcgctttcga aatgcgatgt cattcccttg gcggcgcgcc gagctggtcg
     1801 ttgtacttct tgaggagatc gtcgagggct tcgcgaagca ggctggcttc gttgcggccg
     1861 agggttttgg cgagcgcagc gagacgttcg agctgagtgc gggtgtagta cgacgtcttg
     1921 gtgatcatcc ggtcgtcggc gaggggtaac gagaccttgt tgcggccggt cgccttggcg
     1981 agatagagag cctgatcggc ctcgcgcacc agctcgatat cggtcgcgcc gttagcgggg
     2041 tagacggcca ggccgatcga gcaggtaatc gccagatccg gccactcatc gagccgcatc
     2101 gccccgatcc ggcggcgtaa ctcctccacc agcgtgaagg cgtcgtccag ccgggtatcc
     2161 ggcagcgctc caccgaactc atcaccgccg tagcggacga tgaccgcgct ctccggcaga
     2221 gtctcgcgca ggatgcgtga gacgccgagc aggatcgcat cccccgcctg ccgaccgtgg
     2281 ctcacgttca gctcgtggaa gctgtccgtg tccatcagga agacactgag cggatcgccg
     2341 gcctcctgcg cctggcggat caggtcttca cgcaacagat gtcggaaata cgcgggcgac
     2401 agcaaccccg tcaatgggtc ggtggttggc atctcgaacg tgctgggata gctgatcatc
     2461 tttcgatccg tcactttcgt actgccaatt acgttggtac gtatatatac atatgtatgc
     2521 caacatacgt cgcttgtcaa gaagcccgag cggcattcac actcgtcaag aagtagggtc
     2581 cggtcttcta ccggccctgc tcgggttccc gaacgcgtcg ggtgg
//
LOCUS       DASUGJ010000564         6622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1620218, whole genome shotgun sequence.
ACCESSION   DASUGJ010000564 DASUGJ010000000
VERSION     DASUGJ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620218"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFV93_12790"
     CDS             complement(<1..197)
                     /locus_tag="VFV93_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166270.1"
                     /translation="MSTIAIPRLRGMYYGWVILLTLSFTEVTSWGILYYSFSVFLTPM
                     QDELGWSIAQMTGAYSAAILIS"
     gene            complement(424..3129)
                     /locus_tag="VFV93_12795"
     CDS             complement(424..3129)
                     /locus_tag="VFV93_12795"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5166271.1"
                     /translation="MPAANEQESPAREILPRDPQLITKLLPPPSPRDLIARPHLAKAI
                     EPKPGQQITLISAPTGFGKTTAMQEWASTTERAVCWVSLDSGDNEPDGFWSYVVSAIE
                     RGQPGFSATARSLLHTPEAVSAEYVVTAIINELAQIPDPVSLILDDYHLISNEIIHRS
                     MRFLIDHLPAHVHLLVAGLSDPPLGLPRLRAQGRLIELRGPDLCFQPDDTAALLIETL
                     GLSLSAADVQNLQQRTEGWVAGLRLAALALRQQSDPAAFIDGFNGNHRSVGDYLADDV
                     LGRQPEEIQRFMIETSILNQMSGPLCDAVSDRHDSQAILETLEHANAFLVPLDDRRQW
                     FRYQGLFADVLRERFARAGDEVIRTIHRRAAIWYYEQRMTRDAMRHALASGDLDLVAE
                     IVEQRACPMLASGELETIKRWIEPLPREMIERRPILGCLRAWVLVLTGRVDEAEPLLD
                     AVEAQMSDDERQAIGEIAAIRAYAARLRHEIPRAIELSRQARALTPASSFSVRRAIAL
                     NYGIALWWRWEAAEAERAFREAADLAEAGGELGAAVVARCHLARIRSDQGYLDQAWEL
                     FQEAAGLAEHWNVSSLPSQSFVHLGMAYVLIERDDLDAAETCLTECIRLGTAGGKIDH
                     VMLAHVELTRVALARGDLDAAHNAIRAAQTTIDQHPVVHLAAEIALVQVMLWLAEGDL
                     AAASTWATETLARAGGQAETLSEPERLALVHLRLAQDEAADAEAILASLHERSAATFP
                     KGWNTCRIVTLILLARVHDAAGRHDAARDTLDEALRIAQPLGHIRTFAEHGAALVPLL
                     VRIQPNAAISRAYLDRLLDACTPATTAMPSATTLGPVTTALTRRELQVLRHIQDGLSN
                     REIAEALFVTVGTVKRHTNSIYSKLAVNSRTQALAKARLLRLIGQ"
     gene            3340..5589
                     /locus_tag="VFV93_12800"
     CDS             3340..5589
                     /locus_tag="VFV93_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166272.1"
                     /translation="MRLLLKPVRLLLVLAMAAALFGSLPVPQVVAAPPANAFFEQTWQ
                     RTDAPVADLAVSRTWVWGPEALTEPAGEPYAEAPGGTRLVQYFDKSRMEINDPAGDTS
                     DLWYVTNGLLAREMITGQLQLGDNQFEAREPAAINIAGDPDDSSGPTYASFLGRLGDP
                     ARDGGSAIDETIDRAGAVGCCVPDSYGAFAGPLAETGHRVASVFWDYLNSSGWIIERD
                     RHATGTLFANPYYATGYPIGEAYWAEVAVAGVRQWVLVQPFERRVLTYTPGNAAGWEV
                     EMGNVGRHYLEWRYGGSALAAPPDWQLPQLPTWTPAGQAVAIPDLDATYDLHISDVDV
                     DTGQLTAHQEIRIDRFNGLRPERLYLQVVPGYFGWFSLDSLTVAGQPASPDVRQEGLI
                     YAVDLPADVGVPFTIALDFRLDVGREATGWGGTSLDNGVLRLGYWFPIISDDHAYSDT
                     LDPSESQVANYDVTVDVAADVVVAHSGAETGRESLDTSRTRYQLSGERMRDFALVLSR
                     GFGVTERTLPSGVVLRYYSHVSNEGGITDEAIGYRQEVSLAAAADAIQQLETLIGPYP
                     YPTYSIVDVGPTMPGGLEFPSLIYINAAYTPLDRLIYHETAHQWLYAIIGNRTLTDGW
                     IDEGGAEFFERGLPTGFSEVPPYPDGGYAYWLDTPASELPDDPARNWYYSIYEQGARF
                     YNDVRTTMGDGAFWSAFRDIYARYAFAIVTPAEMLATFQSHSTTDLRPLYESYFRYAW
                     IWQLSGPGW"
     gene            complement(5662..>6622)
                     /locus_tag="VFV93_12805"
     CDS             complement(5662..>6622)
                     /locus_tag="VFV93_12805"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX5166273.1"
                     /translation="LAAHAVKAGEVLRLRPQDDRGSAYNAATKVWSSAEGATMKVHIS
                     ADMEGTVGVVDWVQVSPPWMEGKGPAEYERARLRMTREVAAAVRGALAAGADEVIVND
                     SHDGMKNILIDELPRGCRLISGHQKDLSMMQGIDEPDISVAFFTGYHAKAGSPKGVLA
                     HTYIGAVDDVRIDGVSVGEYGLNAAVAGDFGVPVTLVTGDDVAIGQVRDLLGRSVIGV
                     QVKTGLAVQTADNIHPELAYELIEAAAKDAVGLAGSVKPFAIHNARIEASFNHQTLAD
                     MAGLGPGIERIGERTVAFTAENGVALMTTWRGMLNRVMTRFAV"
BASE COUNT         1057 a         2238 c         2155 g         1172 t
ORIGIN      
        1 gagatcagga tcgccgccga gtaggcgccg gtcatctgcg caatcgacca gccgagctcg
       61 tcctgcatcg gtgtcaggaa gacggagaag gagtagtaga ggatgcccca ggaggtgacc
      121 tcggtaaacg acagggtcag caggataacc cagccgtagt acatcccgcg cagcctcggg
      181 atcgcgatcg tgctcacgcc gtcaccccct ccccgcgatt ctaacggggg agcgaccgtc
      241 tcgcatcagg gaacgtgcac aagtggattg gtgatctcgc acaaggcctg accccaatcc
      301 aaatcaatcc ccaatccggt acgggcgata tcgcaatatc gcccttcttg acgcacacgt
      361 cgccggggaa gggcgatatt gcgatatcgc ccgtaccgga ttggggattg gcattgagat
      421 tggctactgg ccgatcaggc gcagcaacct cgccttcgcc agcgcctgag ttcggctgtt
      481 gacggccagc ttcgagtaga tgctgttggt gtgccgcttg acggtgccga ctgtgacaaa
      541 cagcgcctcg gcgatctcgc ggttggacag gccgtcctgg atgtggcgca gtacctgtag
      601 ctcccgccgg gtgagtgccg tggtgacagg ccccagcgtc gtcgcggacg gcatggctgt
      661 ggttgcaggc gtgcaggcgt ccagcagccg gtcgagatat gctcgcgaga tggcggcgtt
      721 gggctggatc cggaccagca gcgggaccag cgccgcgccg tgctccgcga acgttcggat
      781 gtggccgagc ggctgcgcga tccggagcgc ctcgtcgagt gtgtcgcgcg ccgcgtcgtg
      841 ccggccggct gcgtcgtgga ctctggccag caggatcagc gtcacgattc gacaggtgtt
      901 ccagcctttc gggaaggtcg ccgcgctgcg ttcgtgcagc gatgcgagga tcgcttccgc
      961 gtcagcggcc tcgtcctggg ccagtcggag gtgaaccagc gcaagccgct ccggctcgct
     1021 cagtgtctca gcctggccgc cggcccgcgc caacgtctcg gtcgcccagg tggacgcggc
     1081 ggcgaggtcg ccctcggcca gccagagcat gacctggacg agcgcgatct cggccgccag
     1141 gtggacgacc gggtgctgat cgatcgtcgt ctgggcggcc cggatggcgt tgtgggcggc
     1201 gtcgaggtcg ccccgcgcca gcgcgacgcg ggtcagttcg acgtgtgcca gcatgacgtg
     1261 gtcgatcttg ccaccggccg ttcccagcct gatgcactcg gtaagacatg tttcagcggc
     1321 gtccagatcg tcgcgctcga tcaggacata cgccatccca aggtggacga agctctggga
     1381 tggcagcgag gagacgttcc agtgctcggc cagaccggcc gcttcctgga acagctccca
     1441 tgcctggtcg aggtacccct ggtcggagcg aatgcgggcc aggtggcagc gcgccacgac
     1501 ggcagcacca agctctccgc ctgcctcggc caggtcggca gcctcacgga acgcgcgctc
     1561 ggcctcggcc gcttcccagc gccaccagag cgcgatgccg tagttcagcg cgatcgcccg
     1621 gcggaccgag aagctgctgg ccggcgtcaa tgcgcgggcc tgtcgtgaca gttcgattgc
     1681 gcgaggaatc tcatgacgga gtcttgccgc gtaggcgcgg atggcggcaa tctcaccgat
     1741 cgcttgccgc tcgtcgtcgc tcatctgtgc ctcgactgcg tcgagcagcg gttccgcttc
     1801 gtcgacccgg ccagtcagca ccagtaccca ggcgcgcagg cagccgagga tcggtcgtcg
     1861 ctcgatcatt tcgcgtggca gtggctcgat ccagcgctta atcgtttcca gctcgccgct
     1921 ggccagcatc ggacaggcgc gttgctcgac aatctcggcg acgaggtcca gatcgccact
     1981 cgccagcgcg tggcgcatcg cgtcgcgggt catgcgttgc tcgtagtacc agatcgccgc
     2041 tcggcggtgg atggttctga tcacctcgtc gccggctcgt gcaaagcgtt cgcgcaggac
     2101 gtcggcaaac aacccctgat agcgaaacca ctggcgtcgg tcgtcgagcg ggacaagaaa
     2161 tgcgttggcg tgctccagcg tctcaaggat cgcctggctg tcgtgccggt cgctaacggc
     2221 gtcgcagagc gggccactca tctggttcaa gatcgacgtc tcaatcatga agcgctgaat
     2281 ctcttccggc tgccggccga gcacgtcgtc ggccaggtag tcgccgaccg aacggtgatt
     2341 gccgttgaac ccgtcgatga aggcggccgg gtcggactgc tgccgcaacg ccagcgcagc
     2401 cagtcgcagg ccggcgaccc agccttcggt tcgttgttgg agattctgaa cgtcggcagc
     2461 gctcagcgac agtcccagcg tctcgatcag gagggccgct gtgtcgtccg gctgaaaaca
     2521 aaggtccggc ccgcgcaact cgatcaaccg cccctgtgcc cgcagtcgcg gtagccccag
     2581 cggcggatcg ctcaggccgg cgacgagcag gtggacgtgc gcgggcagat ggtcgatcag
     2641 gaagcgcatc gagcggtgga tgatctcgtt gctgatgaga tggtagtcgt cgaggatcag
     2701 cgacacggga tctggaatct gcgccagttc gttgatgatc gccgttacga cgtattcggc
     2761 cgagacagcc tccggcgtgt gcagcagcga ccgcgccgtc gctgaaaagc ccggttgccc
     2821 acgctcgatc gctgacacga cgtaggacca gaagccatcc ggttcattgt cgccgctgtc
     2881 cagcgaaacc cagcagacgg cacgctcggt cgtggacgcc cattcctgca tcgcggtcgt
     2941 cttgccgaag ccggttggcg ccgagatgag cgtgatctgc tggcctggct ttggctcgat
     3001 cgcctttgca aggtgaggcc tggcgatcag atcgcggggc gatgggggcg gcagaagctt
     3061 ggtgatcagt tgcggatcgc gcgggaggat ctcgcgagcg ggtgactcct gctcgttcgc
     3121 agccggcatc ggtcggggat ggctcagcat cgtgccagcg cggcctctcc gtcacacccc
     3181 gccgtcggac gagcaggtgt tgcaactgtc cccaatgtcc aacttacgta caatccgccc
     3241 gggaggatgc ctgccgctcc ccagcgttcc cgcgttcgtg cccttccatg tcgagctcgc
     3301 cgggccgcca tcgtggcgct cgaaagcgtg gagaacgata tgcgcctgtt gctgaagccg
     3361 gttcgtctgc tgctcgtgct ggcgatggct gccgccctgt tcggcagcct gccggtcccg
     3421 caggttgttg ccgcgccgcc ggccaacgcc ttcttcgagc agacctggca gcggaccgac
     3481 gcgccggtcg ccgatctggc cgtctcgcgt acctgggtct ggggaccgga ggcgctgacc
     3541 gagccggccg gcgagcccta tgccgaggcg ccgggtggca cgcggctggt ccagtacttc
     3601 gacaagagcc ggatggagat caacgatccg gccggcgaca ccagcgatct ctggtacgtc
     3661 accaacggcc tgctggcgcg cgagatgatc accggtcagc tccagcttgg tgacaaccag
     3721 ttcgaggccc gcgagccggc cgcgatcaac atcgccggtg acccggacga ctccagcggt
     3781 ccgacctacg ccagcttcct cggccggctc ggcgatccgg cgcgggacgg gggctcagcc
     3841 attgatgaaa cgatcgaccg ggccggcgcg gtaggttgtt gcgttcccga cagctacggc
     3901 gccttcgccg gtccgctggc ggagaccggc caccgggtcg ccagcgtctt ctgggattac
     3961 ctcaacagca gcggctggat catcgagcgc gaccgccacg cgaccggtac cctcttcgcc
     4021 aacccctact atgcgaccgg ctacccgatc ggcgaggcgt actgggccga ggtagccgtc
     4081 gccggcgttc ggcagtgggt gttggttcag ccgttcgagc gccgcgtcct gacctacacg
     4141 cccggcaacg cggccggctg ggaggtcgag atgggcaacg tcggccgcca ctacctcgaa
     4201 tggcgctacg gcggctccgc cctggccgcc ccacctgact ggcaactgcc gcaactgccg
     4261 acctggacac cagccggtca ggcagttgcc atcccggacc tcgacgcgac ctacgacctg
     4321 catatcagcg acgtcgatgt tgacaccggc cagctgaccg cccaccagga gatcagaatc
     4381 gatcgattca acggcctgcg gcccgagcgg ctctacctgc aggtcgttcc cggttacttc
     4441 ggctggttta gcctggacag cttgacggtc gccggtcaac cggccagtcc tgatgttcgc
     4501 caggaggggc tgatctacgc ggtcgatctt ccagccgatg tcggggtgcc gttcacgatt
     4561 gcgctcgact ttcgcctgga cgttggccgg gaagcgaccg gctggggtgg tacgtcgctc
     4621 gacaacggcg tcctgcggct cggctactgg ttcccgatca tctccgacga ccacgcctac
     4681 tcagacacgc tcgacccgtc tgagtcgcag gtggcgaact acgacgtaac cgtcgatgtg
     4741 gctgccgatg tcgtcgtcgc ccactccggt gccgagaccg ggcgcgaatc gctggacact
     4801 tcacgaaccc gctaccagct cagtggcgag cggatgcgcg acttcgcgct cgttctctcg
     4861 cggggcttcg gcgtcaccga gcggacgctg ccatctggtg tcgtcctgcg ctactacagc
     4921 cacgtttcga acgagggcgg aatcaccgac gaagcgatcg gctaccggca ggaggtcagc
     4981 ctggcggctg ccgccgacgc aatccagcag ctcgaaacct tgatcggacc gtacccgtat
     5041 ccgacctatt caatcgtcga tgtcggccca accatgcccg gtgggctgga gttccccagc
     5101 ctgatctata tcaacgctgc ctacaccccg ctcgaccggc tcatctacca cgagacggcc
     5161 caccagtggc tctatgccat catcggcaac cgcacgctga ccgacggctg gatcgatgag
     5221 ggcggggcgg agttcttcga gcgcggcctg ccgaccggct tcagcgaggt gccaccctac
     5281 ccggacggcg gctacgccta ctggctcgat accccggcca gcgagctacc cgacgacccg
     5341 gcccgtaact ggtactactc gatctacgaa cagggcgccc gcttctacaa tgacgtccgg
     5401 acgacgatgg gcgacggcgc cttctggagc gccttccgcg acatctatgc tcgctatgcc
     5461 ttcgccatcg tcacgccggc cgagatgctg gccaccttcc agtcccactc cacaaccgac
     5521 ctgcgccccc tctacgagag ctacttccgc tatgcctgga tctggcagtt gtcggggccg
     5581 gggtggtgat ctttcgggac gcgggacgcg ggttgtccat ctatcgcgtc ccgcgtccca
     5641 cgtcccgcgt cacgacaacc ctcacaccgc aaaccgcgtc atcacccgat tgagcatccc
     5701 gcgccaggtg gtcatcagcg cgacgccgtt ttcggcggtg aaggcgacgg ttcgttcgcc
     5761 gatccgttcg atgcctgggc cgaggccggc catgtcggcc agggtctggt ggttgaagct
     5821 cgcttcaatc cgtgcgttgt ggattgcgaa gggcttgacg ctgccggcca gcccgacagc
     5881 gtcctttgcg gccgcttcga tcagctcgta ggccagctcc gggtggatgt tgtcggccgt
     5941 ctggacggcc aggccggtct tgacctggac gccgatgact gaccgcccca gcagatcgcg
     6001 cacctggccg atggcgacat cgtcgccggt caccagcgtc accgggacac cgaagtcgcc
     6061 ggcgacggct gcgttcagcc cgtactcgcc gaccgagacg ccgtcgatcc gcacatcatc
     6121 gaccgcgccg atgtaggtgt gggccaggac gcccttcggg ctgccggctt tagcgtggta
     6181 gccggtgaag aaggcaacgc tgatgtccgg ctcatcgatt ccctgcatca tcgagaggtc
     6241 cttctgatga ccggagatca accggcagcc gcgcggtagc tcatcgatca ggatgttctt
     6301 catgccgtca tgtgagtcgt tgacgatcac ttcgtctgcg ccggccgcca gcgcgccccg
     6361 gacggccgcc gccacctcgc gcgtcatccg cagccgcgcc cgctcgtact cggccggccc
     6421 cttgccctcc atccacggcg ggctgacctg cacccagtca acgacgccga cggttccctc
     6481 catgtcggct gagatgtgga ctttcatcgt cgctccttcc gcgctcgacc agaccttcgt
     6541 tgcggcattg taggcgcttc ccctgtcgtc ctgaggccgc agccgaagga cctccccggc
     6601 cttgaccgcg tgggcggcta ac
//
LOCUS       DASUGJ010000565         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1620722, whole genome shotgun sequence.
ACCESSION   DASUGJ010000565 DASUGJ010000000
VERSION     DASUGJ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620722"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1476
                     /locus_tag="VFV93_12810"
     CDS             232..1476
                     /locus_tag="VFV93_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166274.1"
                     /translation="MTLIIDGFDVQAMGYVIPALSAAWGIEPAQFRTVLPAANFGVLI
                     GSLLFSPIADKIGRRPVLIGSTLWFAVMTLAAAFAGNVTQLFWLRFIGGIGMGCIIPN
                     ATALIGEYSPAGRRVTLMMNITVGFTAGGAVAGFVARWMIPQFGWQSVFLVGGAIPLV
                     ITVAMLASLPESLQFLAVRRRRLDQVARWLKQLDPTIRVDASTEYVAREESKGGVPVV
                     HLFNDRRALVTVLLWIVNFMNLLNLYSVSSWLPTVVNGMGYDQNTSVLVGTVPWVGGT
                     IGTFGLAWIISRKGFTRTLTINYILATFSIALIGQPGISLALLSLIVFVTGWCVIGGQ
                     PGMNAFAATFYPTYLRSTGLGWALGVGRVGAIVGPYIGGSMIGAKWTSQQLFLAAAVP
                     AAISAVTLMILAKVVGDRVKAD"
     gene            1678..>2101
                     /locus_tag="VFV93_12815"
     CDS             1678..>2101
                     /locus_tag="VFV93_12815"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX5166275.1"
                     /translation="MHNSHSSERLAVRQLSDQLQQDIRRLTRSESLGFDRRMRFQIED
                     AAAEVGRNIEQALATDHSGECGRFLRLARSAVNGVKTGLQIARLKRYVVEQDVRPAEE
                     SLSRLYPLLSSLLATASSPSGTPGPLPSYTRPSATRSRI"
BASE COUNT          348 a          681 c          659 g          413 t
ORIGIN      
        1 tgcctgctcg ccaccggctg ggtcgccggc acgacgtccg acctgcgtca ctacaccaac
       61 aaagcgcacc cacaaaacac ctgagtctcg ctcccaggca ccccaggcac tccaagcacc
      121 cgctatactc tgccgcctat gccgagtgcc gcgaactccg tcaacattcc cgagcttctg
      181 gcgaaaagca cgatcggacc gctgcagcgg cgtgtcttcg tgctgtgcct gatgacgctg
      241 atcatcgacg ggttcgatgt ccaggcgatg ggctacgtca tcccggcgct gagcgcggcc
      301 tgggggatcg aaccggcaca attccgcacc gtgctgccag ccgccaactt tggtgtgctg
      361 atcggctcgc tgctcttcag tccgatcgcc gacaagatcg gccgccggcc ggtcctgatc
      421 gggtccacgc tctggttcgc cgtcatgaca ctggccgcgg cgtttgccgg caacgtcacg
      481 cagctgttct ggctgcgctt catcggcggc atcggcatgg gatgcatcat cccgaacgcc
      541 accgcgctca tcggcgagta cagcccggca ggacgccggg tcacgctgat gatgaacatc
      601 actgtcggct tcaccgctgg cggcgcggtc gccggcttcg tcgcccgctg gatgattcct
      661 cagtttggct ggcagtcggt gttcctggtg ggcggtgcga ttcccctggt catcaccgtc
      721 gccatgctgg ccagcctgcc cgagtcgctg cagttcctcg cggtccgtag acgacggctc
      781 gatcaggttg cgcgctggtt gaagcagctc gatccgacga tcagggtcga tgcatcgacc
      841 gaatacgtgg ccagagaaga gtccaaggga ggcgtccccg tcgttcactt gttcaacgac
      901 agacgggcgc tggtgacggt cctgctctgg atcgtcaact tcatgaacct gctgaatctc
      961 tattccgtgt cgagctggct gccgacggtc gtgaacggaa tgggctacga ccagaacacg
     1021 tccgtgctcg tgggtaccgt gccatgggtt ggcggaacga tcggcacctt cggcctggcg
     1081 tggatcatca gccgtaaggg tttcacgcgg acgctgacga tcaactacat cctggcgacg
     1141 ttcagcatcg ccctcattgg acagccgggc atttcactgg cgctgttgtc gctcattgtg
     1201 ttcgtgaccg gctggtgcgt gatcggcggc cagcctggca tgaacgcctt cgccgccacg
     1261 ttctatccga cgtatctccg atcaaccggg cttggctggg cactcggcgt cggccgcgtc
     1321 ggcgcgatcg tcggcccgta tatcggcggg tcgatgatcg gagccaagtg gacgtcgcag
     1381 cagctcttcc tggcagccgc cgttcccgcc gcgatatcgg cggtgacgct catgatcctc
     1441 gcgaaagtcg ttggtgatcg agtgaaagcg gactgacgag ggtcggtcca agcgggatcc
     1501 tggtcggtcc aagcgggatc ctggtcggtc caagtcggct cgcggtcggt gcaaggccgg
     1561 tatcgcggtc ggtgcaatac ctgcgatctc aggcgcggga gaccagccaa gtttctgcgt
     1621 actttcgccg taagtcgttg acagacgccg ggcacgcgcg atgctgctcg cgggtgcatg
     1681 cacaactcac attcgtccga acgcctcgcc gttcgtcagc tctccgatca actgcaacag
     1741 gacatacggc gactcacccg aagcgagtcg ctgggcttcg atcgccggat gcgatttcag
     1801 attgaggacg cggcggcgga ggtcggccgg aatatcgaac aggcgctggc gaccgaccat
     1861 tccggcgaat gcgggagatt tctacggctg gcgcgttccg cggtgaacgg cgtcaagacc
     1921 ggtttgcaaa tcgcgcggct gaagcgatac gtcgtcgaac aggacgtcag gccggcggaa
     1981 gaatcactct cccggctgta ccccttgctg tcgtcgctgc tggctactgc gagctccccg
     2041 tcaggaacac ctggcccgtt gccttcgtac acccgccctt cggcaacgcg gtcccgcatc
     2101 t
//
LOCUS       DASUGJ010000566         3266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2036631, whole genome shotgun sequence.
ACCESSION   DASUGJ010000566 DASUGJ010000000
VERSION     DASUGJ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036631"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFV93_12820"
     CDS             <1..265
                     /locus_tag="VFV93_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166276.1"
                     /translation="GPVAFDRERMQAPHIAVTVFARGLLNHLYTRIYFAGEPANANDP
                     ILQLVPADRRETLIATRHDDDAAIVYRFDIILQGNNETVFFNY"
     gene            358..1122
                     /locus_tag="VFV93_12825"
     CDS             358..1122
                     /locus_tag="VFV93_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5166277.1"
                     /translation="MNIRLLRHATIVVDWGDVSILVDPMLAEKDALDPIPNAANTHRI
                     PMLPLPVDDDALQQIIAGVDGVFVTHTHRDHWDARASELIPHETPMVCQPPDFDRFSG
                     EGYNNLITIQQHERWHGFDVTRTGGQHGTGEIGARMGPVSGFVFRAAGQRTLYIAGDT
                     VWCPEFEDALREHAPDVIVLNAGAAQFNVGDPITMTAEDVMRVANTAPNAKIVAVHMD
                     TINHCLLTRAMLTKVVADAGLSERVLIPGDGETIAL"
     gene            complement(1307..2374)
                     /gene="recA"
                     /locus_tag="VFV93_12830"
     CDS             complement(1307..2374)
                     /gene="recA"
                     /locus_tag="VFV93_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX5166278.1"
                     /translation="MSERDKAIEVALATIERQFGKGSIMKMNESSRLPIEGIPTGSIA
                     LDMALGIGGVPRGRIIEIYGPESSGKTTLAQHIIAEAQKLGGVAAYIDAEHALDPLYA
                     ERCGVNLDNLLISQPDTGEQALEITETLVRSGAIDIIVVDSVAALVPRAEIEGDMGDS
                     HVGLQARLMSQALRKLTGAISRSRTSVIFINQLRMKIGVMFGSPETTTGGQALKFYSS
                     VRMDIRRIETLKQGTDSIGSRVRVKVVKNKVAPPFRQAEFDIMYNEGISKAGSVLDVG
                     VELGIVRKSGAWFYLEEDRLGQGRENAKEFLNNNPDILAEIEHRIRATSPEFAEREAP
                     QDEVLAMAGASNGLFDDDEIV"
     gene            2705..>3266
                     /locus_tag="VFV93_12835"
     CDS             2705..>3266
                     /locus_tag="VFV93_12835"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX5166279.1"
                     /translation="MLQIRLFGGVDLRDDGEPIPSPESARASMLLAHLLLHRDTPQSR
                     ERLAFLLWPDSTESQARTNLRHVLHNLRRALPDPDRFLTVTPQTLQWQPEAGYWLDVA
                     AFETLAAQAEAEPEAQQLAALREAVALYTGDLLDGWYDEWVLDERERFSRQYLQLLER
                     LIDRLEAHGELAEAIQHGEQLLRQDQL"
BASE COUNT          571 a         1099 c         1014 g          582 t
ORIGIN      
        1 ggggccggtc gccttcgacc gcgagcggat gcaggcgccg cacatcgccg tcaccgtctt
       61 cgcccgtggc ctgctcaacc acctctacac gcgcatctac ttcgccggcg aaccggccaa
      121 cgccaacgac ccgatcctgc aactcgtccc cgccgaccgg cgcgagacgc tgatcgccac
      181 ccgccacgac gacgacgccg cgatcgtcta ccggttcgat atcatcctgc aaggcaacaa
      241 tgagaccgta ttcttcaatt actagagaac cataacccta ccccggtagg gacaggtctg
      301 agacctgtcc ctacgggagc gggattgttg ggacggagat tcagagatgg gagacagatg
      361 aacattcgat tgctgcgcca cgcgacgatc gtggttgact ggggtgatgt cagcattctg
      421 gttgacccga tgctggccga gaaggacgcg ctcgacccga tccccaacgc cgccaacacc
      481 caccgcatcc caatgctacc gctgccggtc gatgacgacg cgctccagca gatcatcgcc
      541 ggtgtggatg gtgtcttcgt cacccacacc caccgcgacc actgggacgc ccgcgcgtcc
      601 gagctgatcc cgcatgagac gccgatggtc tgccagccgc ctgatttcga tcgtttcagc
      661 ggcgagggct acaacaatct gatcacgatc cagcagcacg aacgctggca cggcttcgac
      721 gttacccgca ccgggggcca gcacggcacc ggcgagatcg gcgcccggat gggccccgtc
      781 tccggcttcg tcttccgggc cgccggccag cgaacactct acatcgccgg cgacacggtc
      841 tggtgtccgg agtttgagga cgccctgcgc gagcacgccc ccgacgtgat cgtcctcaac
      901 gccggcgctg cccagttcaa cgttggcgac ccgatcacga tgacggccga ggatgtgatg
      961 cgcgtcgcca acaccgcccc gaacgcgaag atcgtcgccg tccacatgga cacgatcaac
     1021 cactgcctgc tgacccgtgc gatgctaacg aaggtggttg cggacgcagg gttgtcggag
     1081 cgcgtgctga ttccggggga tggcgagacg atcgcgttat aacccaatgc aaaccatcaa
     1141 tccggtacgg gcgatattgc gatatcgccc tttcagatgc ccgcgccgcc ggggaagggc
     1201 gatattgcga tatcgcccgt accggattgg gacattggac tgaagattga atgacaacag
     1261 gcgcccaatc gggcacctgt catgcgatct gcgttgtcgt gaaacgctag acgatctcgt
     1321 cgtcgtcgaa gaggccgttg ctggcgccgg ccatcgccag cacctcgtcc tgcggggcct
     1381 cgcgctcggc aaactcgggt gaggtcgcgc ggatgcgatg ctcgatctcg gccaggatgt
     1441 cggggttgtt gttgaggaac tccttggcgt tctcacgccc ctgcccgagc cggtcctctt
     1501 cgaggtagaa ccaggcgccg ctcttgcgga cgatgcccag ctcgacgccg acgtcgagga
     1561 cgctgccggc cttcgagatg ccctcgttgt acatgatgtc gaactcggcc tggcggaacg
     1621 gcggcgcaac cttgttcttg accaccttga cgcggacgcg gctgccgatc gagtcggtgc
     1681 cctgcttgag cgtctcgatc cggcggatgt ccatccggac cgacgagtag aacttgagcg
     1741 cctgaccgcc ggtcgtcgtc tccgggctgc cgaacatgac gccgatcttc atccggagct
     1801 ggttgatgaa gatgaccgac gtccgcgagc gggagattgc gccggtcagc ttgcgcaacg
     1861 cctggctcat cagccgggcc tggaggccga cgtgcgagtc gcccatgtcg ccctcaatct
     1921 cggcccgtgg aacgagcgcg gcgaccgagt cgacgacgat gatgtcgatc gcgccggagc
     1981 gcaccagtgt ctcggtaatc tccagcgcct gctcgccggt gtccggctgg gagatgagca
     2041 ggttgtcgag gttgacgcca caacgctctg catagagcgg gtcgagcgcg tgctcggcgt
     2101 cgatgtaggc ggccacgcca cccagcttct gcgcctcggc gatgatgtgc tgggccagcg
     2161 tcgtcttccc ggacgactcc ggtccgtaga tctcaatgat ccggccacgc gggacaccgc
     2221 caatgccgag cgccatgtcc agcgcgatcg agccggtcgg gatgccttcg atgggcaggc
     2281 gtgaggactc gttcatcttc atgatcgagc ctttgccaaa ctgacgctcg atcgtcgcca
     2341 gtgcgacctc aattgccttg tcgcgttcgc tcactgcggt acctctcatc tggcggaacg
     2401 gtcgttacca gaaagtagaa cggatgatct aaatccttgt cgtattctac tccttttgcc
     2461 cgtcccggtc aatcatgcgc gtctcggaat gccgcgtacg gtcgcgtcct gctgtcgcgc
     2521 gttcgcggcg tctgggtgcg tgtctctatc ctaccgcgaa cagtaccgag tggttgacag
     2581 cgaacggcta ccgccgcaaa cttcccctgc atccatccgt tacgaagatc gcgccgcgcc
     2641 cgcgcaaagg ggcggctacc ggcatcgcgg ccagctatca gcaatgggac cattggagac
     2701 tccggtgctc cagattcggc tcttcggcgg ggtggacctc cgcgacgatg gcgaaccgat
     2761 tccctcaccc gagtcggcgc gcgcctcgat gctactggcc cacctgctgc tgcaccgcga
     2821 cacgccgcag tcgcgcgagc ggctggcgtt cctgctctgg ccggactcga ccgaatccca
     2881 ggcgcgcacc aacctgcgcc atgtcctcca caacctgcgc cgcgccctgc ccgatcccga
     2941 ccgattcctg acggtcaccc cacagacgct ccagtggcag ccggaggcag gttactggct
     3001 cgacgtcgcc gcgttcgaga cgctggccgc gcaggccgag gcggagccgg aggcccagca
     3061 actggccgca cttcgggagg ctgtcgccct ctataccggc gaccttctcg acgggtggta
     3121 cgacgagtgg gttctcgacg agcgggaacg gttcagccgg cagtatctgc agctgcttga
     3181 gcggctcatc gaccggctcg aagcgcacgg cgaactggcc gaggcgattc agcacgggga
     3241 gcaactgctg cggcaggacc agctca
//
LOCUS       DASUGJ010000567         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_509194, whole genome shotgun sequence.
ACCESSION   DASUGJ010000567 DASUGJ010000000
VERSION     DASUGJ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509194"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1024)
                     /gene="jag"
                     /locus_tag="VFV93_12840"
     CDS             complement(137..1024)
                     /gene="jag"
                     /locus_tag="VFV93_12840"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX5166280.1"
                     /translation="MDRMRSVEIQARSAEEAIRLALEQLGCTREQVDVQILAETEDDI
                     YGEGEVLVRVAMRGTAAQPRGDRSQPRGGQQGRGGRGQRTLSDARPRDSRAPRSWQGG
                     AQRHRQPSHGSVSGVPRHPQPVRDRQPSQASDEKTQAVETLAKSVVRELLTHMDVHAD
                     VMAVDNPSAMPLSHDDPLTVFVDIMGRDLAMLIGRRGENLSQLQYMVNLLVNKRSEEW
                     VRVILDVEGYRSQREESLIGLAERVARQVARNRRPISLEPMPPNERRIVHMTLKEMGD
                     VRTESSGEGEMRRVTIYPL"
     gene            complement(1123..2145)
                     /locus_tag="VFV93_12845"
     CDS             complement(1123..2145)
                     /locus_tag="VFV93_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX5166281.1"
                     /translation="MLGPWHAYVDFIEWCLQRLTDFTGNGGLAIILFTILLKTVMLPL
                     TVKSIKSTAAMQELQPKIKELQKKYAKDRTRLTEETMRLYQEYQINPAAGCLPMIIQI
                     PIFFGLYFAVKDLSKTAGEFLWISNLNDPDPYKILPIMAGIFQFAQSRMMRPANAPKV
                     TDPQQAMMNTMMNFMPIMVVIFGWTFAAGAVLYWATQSVYSVVQQWLITGFGSLKNWF
                     PFLEKFELPEHRRLGHRKQPLERTEVQGEVKQKGLMGYLQRQAKQAEERRAGAAATRD
                     DTPAARSGSSGKRAKQANVRNVSGAAEDDGGETDETPPPGSTTSKTRVMSGPAARNQR
                     RTRGNK"
     gene            complement(2243..2452)
                     /gene="yidD"
                     /locus_tag="VFV93_12850"
     CDS             complement(2243..2452)
                     /gene="yidD"
                     /locus_tag="VFV93_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX5166282.1"
                     /translation="MRRLALLLIRGYQRGISPMLPPSCRFVPSCSDYGYQAIEKYGII
                     RGGAMTVWRIMRCNPFNKGGFDPVP"
     gene            complement(2449..2835)
                     /gene="rnpA"
                     /locus_tag="VFV93_12855"
     CDS             complement(2449..2835)
                     /gene="rnpA"
                     /locus_tag="VFV93_12855"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX5166283.1"
                     /translation="MGPGIWPEILPMVARELRIRRGSDIDRVRSKGRSWSSRLLVLAI
                     LPNGLEHNRYGFTVGKRVGGSVERNRAKRLMREAIRHRHPTLVQGHDVLLIARNSFRD
                     ETTLAEVAEQLDGLLRRAGIRSAETG"
     gene            complement(2887..>3011)
                     /gene="rpmH"
                     /locus_tag="VFV93_12860"
     CDS             complement(2887..>3011)
                     /gene="rpmH"
                     /locus_tag="VFV93_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX5166284.1"
                     /translation="DHGFMKRMSTRAGRAVLAARRRKGRHSLSVSTEYKPTQKK"
BASE COUNT          461 a          968 c          989 g          593 t
ORIGIN      
        1 cggtcgatgg tcatgtggcc gatggcgacg taatccggga cgcggccgct gtggctggcg
       61 gtcattcgct ccatgctttc aacgccggta tgggttgcat gaccacaccg gcgctcactc
      121 ggcccgtcag cgacggctag agcgggtaga tcgtgacgcg gcgcatctcg ccctcgccac
      181 tcgactcggt ccggacgtcg cccatttcct tgagtgtcat gtggacgatg cggcgctcgt
      241 tgggcggcat cggctccagc gagatcggtc gccggttgcg ggcgacctgg cgggcgaccc
      301 gctcggccag cccgatcagc gattcctcgc gctgcgatcg gtagccctcg acatcaagaa
      361 tgacgcgcac ccactcctcg ctgcgcttgt tgaccagcag gttgaccatg tactgcagct
      421 ggctcaggtt ctctccgcgc cggccgatca gcatcgccag atcgcgaccc atgatgtcaa
      481 cgaagacggt cagcgggtcg tcgtgggaga gcggcattgc cgaggggtta tcgacggcca
      541 tcacatcggc gtggacgtcc atgtgtgtca gtagttcgcg gacgaccgat ttcgccagcg
      601 tctcgacggc ctgggtcttc tcgtcggaag cctgcgaggg ctggcggtcg cgcaccggct
      661 gcgggtgacg tggaacgccc gagacggagc cgtggctcgg ctggcggtga cgctgggcgc
      721 cgccctgcca ggagcggggc gcgcggctgt cacgaggcct ggcgtcggag agcgtgcgct
      781 ggccgcgccc gccacgcccc tgctggccgc cgcgtggctg gctgcggtcg ccgcgtggct
      841 gggcggccgt gccgcgcatc gcgacgcgaa cgaggacttc gccctcgccg tagatgtcgt
      901 cctcggtttc ggccaggatc tggacatcga cctgctcgcg agtgcaaccc agttgttcga
      961 gcgcgagccg gatcgcctct tcggccgacc gggcctgaat ctcaacgctg cgcattcgat
     1021 ccatctgtgc tgctccctca cgttgtcctg ctgacgccgt tacagcaggg atggcatcgt
     1081 cgccggatga ggccaggctg gcaagctggc gcgtctgccc ggctatttgt tgccgcgtgt
     1141 gcgtcgctgg ttgcgcgcgg ccggcccgga catgacacgc gtcttgctgg tcgtgctgcc
     1201 aggcggcggc gtctcgtcgg tttcgccgcc atcatcttcg gcggcgccgc taacattgcg
     1261 gacattggcc tgtttggcgc gtttaccgct cgatcccgat ctggcggccg gcgtgtcatc
     1321 acgtgtggcg gccgcaccgg cacggcgctc ttcggcctgc ttggcctggc gctgcaggta
     1381 gcccatcagg cccttctgct tgacctcgcc ctggacttcc gtccgctcaa gcggctgctt
     1441 gcggtgtccc agccggcggt gctcgggcag ctcgaacttt tcgaggaacg ggaaccagtt
     1501 cttcagcgag ccaaagccgg tgatcagcca ttgctgcacg accgagtaga cgctctgcgt
     1561 ggcccagtag aggacggcgc cggccgcgaa ggtccacccg aagatgacga ccatgatcgg
     1621 catgaagttc atcatcgtgt tcatcatggc ctgttgcggg tcggtcacct tcggcgcgtt
     1681 ggccggccgc atcatccgcg attgggcgaa ttggaagata ccagccatga tcggcaggat
     1741 cttgtaggga tcgggatcgt tcaggttaga gatccacagg aattcgccgg ccgtcttgga
     1801 gaggtctttg acggcgaagt agaggccgaa gaagatcgga atctggatga tcatcggcaa
     1861 gcagccggcg gctgggttga tctggtactc ctggtagagg cgcatcgtct cttcggtgag
     1921 ccgcgtccgg tccttggcgt acttcttttg cagctccttg attttcggct gcagctcctg
     1981 catcgctgcc gttgacttga tcgacttgac ggtcagcggc agcatgacgg tcttgagcag
     2041 aatcgtgaac aggatgatgg ccagaccgcc gttgccggtg aagtcggtca gccgttgcag
     2101 acaccactca ataaaatcaa catatgcgtg ccacggaccg agcaatggtc ggtattcctc
     2161 tcaggttccg gcgcgggccg gattcagaat gaagcgcggg tcgtcaatgc ctcgctacgg
     2221 tcgcgcttgt cgtgcgacga tgtcatggga cgggatcgaa tccacccttg ttgaacggat
     2281 tgcagcgcat gatgcgccag accgtcatcg cgccgccccg gatgataccg tacttctcga
     2341 tcgcctggta gccgtaatcg gagcagcttg gcacgaatct gcacgagggc ggcagcatcg
     2401 gtgagatgcc gcgctggtag ccgcgtatca gcagaagtgc gagccgtctc atcctgtttc
     2461 cgccgatcgg ataccggccc gccgcagcag gccatccagt tgctccgcca cctccgcgag
     2521 tgtcgtctca tcgcggaagc tgttgcgtgc gatcagcagc acatcgtgtc cctgcaccag
     2581 tgtcgggtga cggtgccgga tcgcctcacg catcagccgc ttggcgcggt tgcgctcgac
     2641 tgatcccccc acccgcttgc cgaccgtgaa gccgtagcga ttgtgctcca acccgttcgg
     2701 caggattgcc agaaccagta atcgcgacga ccagctccgg cccttcgagc gtacgcggtc
     2761 gatatcgctt ccgcgtcgga tgcgtagctc acgggcaacc atcggcagta tctccggcca
     2821 gatgccagga cccacccggc tggcccgaca ggccagggtg ggtcctcggg cgactcacgg
     2881 ccggcgctac ttcttctgcg tcggcttgta ctcggtgctc acgctcagcg aatggcgccc
     2941 cttgcgccgc cgcgcggcca gcacagcgcg cccggcgcgg gtggacatgc gcttcatgaa
     3001 accatgatcc t
//
LOCUS       DASUGJ010000568         7598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1341413, whole genome shotgun sequence.
ACCESSION   DASUGJ010000568 DASUGJ010000000
VERSION     DASUGJ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341413"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..188)
                     /locus_tag="VFV93_12865"
     CDS             complement(18..188)
                     /locus_tag="VFV93_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166285.1"
                     /translation="MSWIVLVIALVMAFVVLIVQLLTGHRALWLVSSLLRMASVAWRM
                     ALPKRAVPRSQK"
     gene            318..1076
                     /locus_tag="VFV93_12870"
     CDS             318..1076
                     /locus_tag="VFV93_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5166286.1"
                     /translation="MCYDDSARPPLPPVSGGSGGLASSGDLTLTAADGTEFMAYAARA
                     AEPTGTGMVILPDVRGLHSFYKELAERFAEIGIDAVAYDYFGRTAGSGNRDEGFEYRP
                     HVEQTTPAGVDADCAAAIDYLRSEAGGGVSRVFTVGFCFGGAASWRQSASQPGLDGAI
                     GFYGVPARVRDYMPQMQAPLLLLVAGADFTPTEEFEKFSGELDAAGVPNRMVVYEGAP
                     HSFFDRTFTEHAAACEDAWHQILDFIQQPAPVSS"
     gene            1099..1968
                     /locus_tag="VFV93_12875"
     CDS             1099..1968
                     /locus_tag="VFV93_12875"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5166287.1"
                     /translation="MNGSTLGVIGLGRMGSPIAGRLVVAGYRVLGFDRAGTNERLPDG
                     AMAAASVAAMAGEAETILLSVPDGAASLAVSREIAATADRRVRTVVDLSTIGIAAARE
                     CADLLAGAGVAYVDAPVSGGVAGARGGTLAMMVGAAESLVTELDPILAVVAAKRFRVG
                     DAPGQGQAMKLLNNYASAAALAATCEATVFGARLGLDLAAMVDVINASSGRSAASEDK
                     FPRSIVPGSYDFGFAGALMTKDVSLYLDNAAASGVPHELADAVAAIWRRFNAADPDAD
                     FTALHRYLAEGGS"
     gene            2074..3240
                     /locus_tag="VFV93_12880"
     CDS             2074..3240
                     /locus_tag="VFV93_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX5166288.1"
                     /translation="MTGTSDTIQRFTIAIPQADLDDLRDRLARTRWPGELPGAGWNYG
                     VPVGYLKDLADYWQTGYDWRVHEAALNAYPQFTTTIDGQNVHFLHVESPEPDALPLVI
                     THGWPGSIAEFLKIIGPLSDPRSHGGDPADAFHLVIPSMPGYGFSGPATEPGWTTDRV
                     ATAWAELMRCLGYERYGAQGGDWGAFVAPALGRADSEHVVGVHVNAASVGFIPMGEVP
                     AEELATFTELEKTRLERLNHFLSDQNGYFQIQATRPQTLAYGLTDSPVGQLAWIIEKF
                     KEWTDPPESLPEAAIDRDHLLTNVMLYWLTRTAGSSARMYYANMHSGSWGDPPGTTPT
                     GVAVFAQDVSIRRYAEQGNNIVHWSDFDRGGHFAAMEVPDLLVGDVRAFFRSLQ"
     gene            complement(3278..3541)
                     /locus_tag="VFV93_12885"
     CDS             complement(3278..3541)
                     /locus_tag="VFV93_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166289.1"
                     /translation="MRFPSDGSFWCRNHLQGPIPIAIGGKRVSLNDWHLPTGWVPIEE
                     VIRFCIVDLGVEPLDRSLDANDNSGWHTRLTESYQQSTSSFVA"
     gene            4039..7209
                     /locus_tag="VFV93_12890"
     CDS             4039..7209
                     /locus_tag="VFV93_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX5166290.1"
                     /translation="MAEATVNPVLYGGMEYRCIGPHRGGRVVAVAGDPAHKQTFYFGS
                     TGGGVWKSQDGGKSWRNVSDGFFKTASVGALQVAASDPNVIYAGMGETSIRGNVSRGD
                     GVYKSTDAGRTWQQMGLENTQNIGEIQIHPTNPDIVYVAAFGHVWGENQERGVYRSTD
                     GGKTWTNVLFKSGKAGAVDLSMDPTNPRILYAAIWEANRGPHFMSSGGEDSGLWRSTD
                     GGDSWTDISRNPGLPKEGVLGKIGVAASGAQAGRVYAIVEHENGALFRSDDWGDTWTR
                     GSEDRNLRTRAWYYHHIYADPNDADVVWILNVQLWKSIDGGRNFTAVPVQHGDTHDFW
                     IDPDDSNRIILGDDGGAEVSFTGGTSWSTIFNQPTAEFYHVTVDSREPYRVYGAQQDN
                     TTMSVPSRSNYGFISTSEWYPIGGGESGYVAVKPDDPDLVFAGSYGGLLTRYDARTRL
                     AKAITIWPEMMLGSAASEMKYRFQWTSPTVFSPHDPNVLYHGGNHVFRTTNQGHSWDR
                     ISPDLTRADPESLVSSGGPITKDNTGAETYATVFAIAESPVEKGLIWTGSDDGLIYLS
                     RDGGGNWQNVTPGPDLLPEWSLISIVEPSHFEAGTCYVAATRYKSHDDAPYLLRTDDY
                     GASWQAINTGIPEGEFTRVIREDTEVMNLLFAGTERGLYVSFDRGDTWQSLQLNLPVV
                     PIHDLIIAQGDLVVATHGRSFWVLDDISPLRQLADGAVTENGTVLYTPRASKRWASSP
                     GFGGGAVPGRNFTSAGGLSTSFEQITTSEGKPKKVSLDAGDNPPDGVLINYYLADAPK
                     DPITLRILDGDGNLVREFSSKELDEKEFKDNPGKSRPPVVPAKQGGNRFVWDLRYPNA
                     TEVPDDTGSMGFARGATGPKAEPGSYQVEIINGGTTLTQPFDLLPDPRTGSSVENYRE
                     GFELAMQVRDKLSELHEGVNKLRAVRKQVDAWSERLGDEGVTKAGEALKDQLREVEDQ
                     LIQWRAKAGQDTLNFPVMLNAKLAALIGSINGMEGTPTEQSREVYADLAGKVDTQLKK
                     LDTILKKEVPAFNAKVKAVAADAIAI"
     gene            complement(7290..>7598)
                     /locus_tag="VFV93_12895"
     CDS             complement(7290..>7598)
                     /locus_tag="VFV93_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5166291.1"
                     /translation="AGQIVVTIEDIVLAEVVWTLRSFYRAERVAIADQLIELMSDDHV
                     TNDDKPSLLLALTLFRKHNLGFADAILAARSLIRGDTEIISFDRGLDRVPGLVRREPS
                     "
BASE COUNT         1345 a         2438 c         2513 g         1302 t
ORIGIN      
        1 cggcacaaga ccggccccta cttctgagag cgcggaactg ctcgtttcgg tagagccatt
       61 cgccacgcca ctgatgccat tcgcaacagg ctggatacca gccagagcgc acggtggccg
      121 gtcaggagtt ggacgatcag gacgacgaac gccatcacca gcgcgattac cagaacaatc
      181 cagctcattg agtgactcct catttacgat cgtgcgaatc cagtcgacac gggatgcacg
      241 cgcgatgccc gctggcgttg cggcgcggtc cgtgctacaa gacggcaacc gccaacgcat
      301 gagaaggagc gaacccgatg tgctatgacg attcggcccg accgcccctc ccgcccgttt
      361 cgggtggttc cggtggcctg gcatccagcg gcgatctgac actgacggcg gctgacggca
      421 ccgagttcat ggcctacgcc gcgcgggcgg ccgagccgac cgggaccggg atggtgatcc
      481 tgccggatgt tcgcgggctg cacagcttct ataaagaact ggccgagcga ttcgcggaga
      541 tcggtatcga cgccgttgcc tatgactact tcggccggac ggccggcagc ggcaaccgcg
      601 acgaaggctt cgagtatcgt ccgcacgtcg aacagacgac gccggccggt gtcgacgccg
      661 actgtgccgc cgcgatcgac tatctgcgtt cggaggcggg tggcggtgtc tcgcgggtct
      721 tcacggtcgg cttctgcttc ggcggcgcgg cctcgtggcg gcagtcggcg tcccagccgg
      781 ggctggacgg cgcgatcggc ttctatggcg tcccggctcg cgtccgcgac tacatgccgc
      841 aaatgcaggc gccgctcctg ctgctggtgg ccggggccga cttcacgccg accgaggagt
      901 tcgagaagtt cagtggcgag ctggacgcgg ccggtgtgcc gaaccggatg gtcgtctacg
      961 aaggcgcgcc gcactccttc ttcgaccgca ccttcaccga gcacgcagcc gcctgcgagg
     1021 atgcctggca ccagatcctc gacttcatcc agcagccggc gccggtcagc tcctgaacgc
     1081 tgatgacgga gtgagtccgt gaacggcagc acgctggggg tcatcggtct cggtcggatg
     1141 ggcagcccga tagccggccg gctcgtcgtg gccggctatc gggtgctcgg cttcgatcgg
     1201 gccgggacaa atgagcggct accggatggc gcgatggccg cagcctcggt cgccgcgatg
     1261 gcaggcgagg ccgagacgat cctcttgagc gtgccggacg gcgcggcctc gctggccgtc
     1321 tctcgcgaga ttgctgcgac cgccgatcgg cgcgtcagga cggtcgtcga cctctcgacg
     1381 atcgggatcg cagcggctcg cgagtgcgcc gatctgctgg ccggtgccgg ggtcgcctac
     1441 gtcgatgctc cggtcagcgg cggcgtagcc ggcgcccgag gtgggacgct ggcgatgatg
     1501 gtcggggcag cggaatcgct cgtcaccgag cttgatccga tcctggctgt tgtggcagcc
     1561 aagcgcttcc gggttggcga cgcgcctggc caggggcagg cgatgaagct gctcaacaac
     1621 tacgcctcag ccgccgcgct ggccgcgacc tgcgaggcaa ccgtcttcgg cgcacggctg
     1681 gggctcgatc tggcagcgat ggtcgatgtg atcaacgcgt cgtcggggcg aagcgccgcc
     1741 agcgaggaca agttcccccg cagcatcgta cccggcagct acgacttcgg cttcgccggc
     1801 gcgctgatga ccaaggatgt cagcctctac ctcgacaacg cggcggcgtc cggcgtgcca
     1861 cacgaactcg ccgacgctgt cgcggcgatc tggcggcgct tcaacgccgc tgatcccgac
     1921 gccgatttca ccgctcttca cagatacctt gctgagggtg gctcctgaat agtcgtccac
     1981 ggtgatgttg tgggtaggtt tcctgtcgtt caccgcccaa cggacaaccg gcagcgaatc
     2041 gtcattcata cagatgctgg gaaaggagcc ttgatgacag gcacgtccga caccatccag
     2101 cggttcacaa tcgcgattcc gcaagctgac ctcgatgacc tgcgtgaccg gctcgcacgt
     2161 acccgctggc cgggcgagtt gcctggcgcc ggctggaact acggcgtccc ggttggatac
     2221 ctcaaggatc tggccgacta ttggcagaca ggctatgact ggcgcgtgca tgaggcggcg
     2281 cttaacgcct acccgcagtt cacgacgacg atcgacgggc agaatgttca cttcctgcac
     2341 gttgagtcgc ccgagccgga tgccctgccg ctggttatta ctcacggctg gcccggatcg
     2401 atcgccgaat tcctcaagat catcggcccg ctgagcgatc cccgttccca cggcggcgac
     2461 ccggctgacg cgttccacct ggtcatccca tcgatgcccg gctacggctt ctccggcccg
     2521 gcgaccgagc cgggctggac gacggatcgt gtcgccactg cctgggctga actgatgcgc
     2581 tgcctcggct acgagcgata cggcgcacag ggcggggatt ggggtgcgtt tgtcgccccg
     2641 gcgcttggcc gggctgattc ggagcacgtt gtcggtgtcc acgtcaacgc ggcgtcagtc
     2701 gggttcatcc cgatgggcga agttccagcc gaggaactgg cgacgtttac cgagctggag
     2761 aagacgcgac tggagcggct caaccacttc ctgagcgatc agaacggata tttccagatt
     2821 caggcaacgc gcccgcagac actcgcgtat gggttgacgg actcgccggt tggccagctt
     2881 gcctggatca tcgagaagtt caaggagtgg accgatccgc ccgagagcct gcctgaagcc
     2941 gccatcgacc gcgaccacct gctgacgaat gtgatgctct actggctcac ccgcacggct
     3001 ggctcgtcgg cgcgcatgta ctacgcgaac atgcattccg gctcgtgggg cgatccgccc
     3061 ggtacgacac cgaccggcgt ggccgtcttc gcccaggatg tctcgatccg gcgctacgcg
     3121 gagcagggca acaacatcgt ccactggtcc gactttgacc gtggcggcca cttcgccgcg
     3181 atggaggtgc cggatctgct ggtcggcgac gtacgggcgt tcttccgcag cctgcaatag
     3241 ccccagggcg ggcgaggcga ctgtcagatc tgcaggatta cgcaacgaac gatgaggtgg
     3301 attgctggta gctctcggtc agccgcgtgt gccaacctga gttgtcgttc gcgtcgaggc
     3361 tccgatcgag tggctccacg ccgagatcga cgatgcagaa tcgaatgacc tcctcgatcg
     3421 gcacccagcc ggtcggcagg tgccagtcgt tgaggctgac tctctttcca ccgatcgcga
     3481 tcgggatcgg cccctgaaga tggttccggc accagaacga gccgtcgctg gggaatctca
     3541 caagagcggc atccgggtga tggtgtgagc gagaagtcta ttgagcgatt ccctgtaatt
     3601 cctgcttgct tcgcggagtg tacgtccctc gaattgcatt ggcggactag tcgttctcag
     3661 gcgttggtgc gtcgatgatc gaggcgatga actccagatc gaagccaatc gagttcttcg
     3721 gcaacgtccc gcaacgcgcg gctgcgattg cttcgtcgaa cgtcagcccg atgctttcgg
     3781 cctcgatctc gaagagttgc cggaactgtt ccttgctgag ttcgccatca ccctcaatcg
     3841 ccgccagcag gtcgggggca tctgaattgt cctggttgcc atcgataccg tccgttcctg
     3901 ctagatcaca tcatcataac cactatatca ctgtcatcca tgacgccttt tcgtaccgga
     3961 cggcaggcaa ccgtggcgca gactggacta gaatggctgg cagaaccaac gcgcgcgagc
     4021 agggaggaag gggcgcagat ggcggaagcg acggttaacc cggtcttgta cggggggatg
     4081 gagtatcgct gtatcggacc gcatcgtggc gggcgtgttg tggccgtggc gggcgacccg
     4141 gcccacaagc agacgttcta cttcggctcg accggcggcg gcgtctggaa gtcgcaggat
     4201 ggcggcaagt cgtggcgcaa cgtctcggac ggcttcttca agaccgcctc ggttggggcg
     4261 ttgcaggtcg cggcgtccga cccgaacgtg atctatgccg ggatgggcga gaccagcatt
     4321 cgtggcaacg tctcgcgcgg tgatggggtc tacaagtcga ctgacgccgg ccggacctgg
     4381 cagcagatgg ggctggagaa cactcagaac atcggcgaga tccagatcca tccgacgaac
     4441 cccgacatcg tctacgttgc ggctttcggt cacgtctggg gcgagaacca ggagcgcggt
     4501 gtctaccgct cgaccgacgg cggcaagacg tggacgaacg tgctgttcaa gagcggcaag
     4561 gccggcgcgg tcgatctgtc gatggatccg actaacccgc gaatcctgta tgccgcgatc
     4621 tgggaggcga accgcgggcc gcacttcatg tcctccggtg gtgaggactc cggcctctgg
     4681 cgctcgaccg acggcggcga tagttggacc gatatctcgc gcaaccccgg cctgccgaag
     4741 gaaggcgtgc tgggtaagat cggcgtcgcg gccagcgggg cgcaggccgg gcgggtctac
     4801 gcgatcgtcg agcacgagaa cggggcgctc ttccgctccg atgactgggg cgacacctgg
     4861 acgcgcggca gcgaggaccg caacctgcgc acccgcgcct ggtactacca ccacatctac
     4921 gccgacccga acgatgccga cgtcgtctgg atcctgaatg ttcagctctg gaagtcgatc
     4981 gacggcggcc ggaacttcac ggccgttccg gtccagcacg gcgacacaca cgacttctgg
     5041 attgacccgg acgattccaa ccggatcatc ctcggcgacg acggtggcgc cgaggtgtcg
     5101 ttcacgggcg ggacgagctg gtcgacgatc ttcaaccagc cgacggccga gttctaccat
     5161 gtgaccgtcg acagccggga gccgtaccgc gtctacggcg cccagcagga caacacgacg
     5221 atgtcggtcc cgagtcgctc gaactacggc ttcatctcga cctccgagtg gtacccgatc
     5281 ggcggtggcg agtccggcta tgtcgctgtc aagccggacg atcccgacct cgtcttcgcc
     5341 gggtcgtatg gcgggctgct gacgcgctat gacgcgcgaa cgcggctggc gaaggcgatc
     5401 acgatctggc cggagatgat gcttggctcg gccgccagcg agatgaagta ccgcttccag
     5461 tggacctcac cgaccgtctt ctcgccgcac gatccgaacg tcctctacca cggcggcaac
     5521 cacgtcttcc ggacgaccaa ccagggccac tcgtgggacc gcatttcgcc ggacctgacg
     5581 cgcgccgatc ccgagtcact cgtctcctcc ggcgggccga tcacgaagga caataccggc
     5641 gccgagacct acgcgaccgt cttcgcgatc gcggaatctc cggtcgagaa ggggctgatc
     5701 tggaccggct cggacgacgg gctgatctac ctctcccgcg atggcggcgg taactggcag
     5761 aacgtcacac cgggcccgga tctcctgccg gagtggtcgc tgatctcgat cgtcgagccg
     5821 tcgcacttcg aggccggcac ctgctacgtc gcggccaccc gttacaagtc gcacgacgac
     5881 gcgccctacc tgctgcggac cgacgactac ggcgccagct ggcaggcgat caacaccggc
     5941 atcccggagg gggagttcac gcgcgtgatc cgcgaggaca cggaagtgat gaacctgctc
     6001 ttcgccggaa ccgagcgggg gctgtacgtc tcgttcgacc gtggtgacac ctggcaatcg
     6061 ctgcagctca acctgccggt cgtgccgatc cacgatctga tcatcgccca gggcgatctg
     6121 gtcgtcgcca cccacggccg ctcgttctgg gttctcgatg acatctcacc gctgcggcaa
     6181 ctggccgacg gcgcggtgac ggagaatggc acggtcctct acacgccacg ggcgtcgaag
     6241 cgctgggcgt cgtcgccggg cttcggcggt ggggccgtgc cgggtcgcaa cttcacctcg
     6301 gcaggcggtc tgtcgaccag cttcgagcag atcacgacca gcgagggcaa gccgaagaag
     6361 gtctcgctcg acgccggcga caacccaccg gacggcgtcc tgatcaacta ctacctcgcc
     6421 gacgctccga aggacccgat cacgttgcgg atcctcgatg gcgatggcaa cctggtccgc
     6481 gagttctcca gcaaggagct ggacgagaag gagttcaagg acaaccccgg caagagccgg
     6541 ccgccggtcg tcccggccaa gcagggcggc aatcgcttcg tctgggacct gcgctacccg
     6601 aatgcgaccg aggtgcccga cgacaccggc tcgatgggct tcgcacgcgg cgcgaccggc
     6661 ccgaaggcgg agcccggctc gtatcaggtc gagatcatca acggcggcac gacgctgacg
     6721 cagccgttcg atctgctgcc cgatcctcgg actggctcgt cagtcgaaaa ctaccgcgaa
     6781 gggttcgagc tggcgatgca ggtgcgtgac aagctctcgg agttgcacga gggtgtcaac
     6841 aagctgcggg ctgtccgcaa gcaggtcgat gcctggtcgg agcggctggg agacgagggc
     6901 gtcaccaagg cgggtgaggc gctcaaggat caactgcgcg aggtcgagga ccagctcatc
     6961 cagtggcggg cgaaggccgg ccaggacacg ctcaacttcc cggtcatgct caacgccaag
     7021 ctggccgcct tgatcggctc gatcaacggc atggaaggca caccgaccga gcaatcccgc
     7081 gaggtctacg cggacctcgc cggcaaggtc gacactcagc tcaagaagct cgacacgatc
     7141 ctcaagaagg aagtgccggc cttcaacgcc aaggtcaagg ccgtcgcagc cgacgcgatc
     7201 gcgatctagc gctcacgtga tctgctgaac gtgaagagcc ggggaggaca gaccctcccc
     7261 ggctcttcgc tgtggtggca tgcacggcgt caggacggtt cgcgtctgac gagaccgggc
     7321 acgcggtcga gtccacgatc aaacgagatg atctccgtat cacctcggat cagcgagcgc
     7381 gccgcgagta tcgcatcggc gaagccgagg ttgtgcttgc ggaagagcgt gagtgcgagc
     7441 agcagcgatg gcttgtcatc gttggtgaca tggtcgtctg acatcagttc gatgagctgg
     7501 tcggcgatgg cgacgcgttc agcgcgatag aacgagcgga gcgtccagac gacctcggcc
     7561 aggacgatgt cttcgatcgt gacgacgatc tgaccagc
//
LOCUS       DASUGJ010000569         7294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2037401, whole genome shotgun sequence.
ACCESSION   DASUGJ010000569 DASUGJ010000000
VERSION     DASUGJ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2037401"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFV93_12900"
     CDS             complement(<1..732)
                     /locus_tag="VFV93_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5166292.1"
                     /translation="MSRYGFVIDHRRCIGCHACTVACKSENEVPVGVFRTWVKYTEKG
                     QFPNSRRFFTVERCNHCDNAPCVEICPTKALFRRPDGIVDFDPSRCIGCKSCMQACPY
                     DAIYIDPFTNTAAKCNYCAHRVDVGLLPACVVVCPAKAIIAGDIDDPTSDIHALISRE
                     QVSVRKPEQGTQPKVFYIGADDASLTPEALTWSNSYLWGELREDGRPQEAVTGDARLS
                     GPAEATVVYDVHHPKPWGWKVATYLW"
     gene            complement(897..1037)
                     /locus_tag="VFV93_12905"
     CDS             complement(897..1037)
                     /locus_tag="VFV93_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166293.1"
                     /translation="MRDLFGPRSYKGGRIQVWGCSPGCLILSIVVSVLLTILLNLALG
                     AF"
     gene            1196..1555
                     /locus_tag="VFV93_12910"
     CDS             1196..1555
                     /locus_tag="VFV93_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HEX5166294.1"
                     /translation="MINDSGVVQPPTMYEQVFGTPAERRERARKRLSIVRWFALLDLI
                     LIVTLTAASFAGNRELVGIIGPIHGGNFVLLLVLVGTAAADGLWSWWFPAAVLLTGGP
                     VGALIGERLVSRRLARD"
     gene            1555..1998
                     /locus_tag="VFV93_12915"
     CDS             1555..1998
                     /locus_tag="VFV93_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166295.1"
                     /translation="MDTTLTWLVRYGHVLFAAGWLGGYAALALAIVPLLQRERTAGLA
                     RLAVGTARTLTYIGAVTMFFGVFLITRTRGLDNLIGGEWGTIVISCIVIAIALAGIGD
                     GPLRQSLVHMIDTGDGTAARRWALVGFGLAVLAIGLMTRAVYASS"
     gene            2016..2726
                     /locus_tag="VFV93_12920"
     CDS             2016..2726
                     /locus_tag="VFV93_12920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5166296.1"
                     /translation="MSTSESEILREQQEYYNARAAEYDEWWMRVGRYDRGAEENSIWF
                     AEQEEVRSAFDTLDWGGEILELAGGTGIWTEWLASRARHITVLDGSLEMIAINSVRLE
                     AGGYRGKADYHQVNLFDWQPERQYDGLFMGYFISHVPTAMMDSFLATVASALKPGGVI
                     GLLDGRRETRSTSSDQPPPGPESDLTMRRLNDGRRFQIVKRFDEPAPLTAALARQGID
                     ADVRTTAIHFLYATGHKQ"
     gene            3295..3732
                     /locus_tag="VFV93_12925"
     CDS             3295..3732
                     /locus_tag="VFV93_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX5166297.1"
                     /translation="MAGTTKTANVTWSGSLMEGKGTIDSVGSGAISNLGVTWKNRAEA
                     AEGTSPEELIAAANAACFAMALSAGLAGAGHAPEKLEVTAKCTFSLDNGPKITTMDLH
                     VRGTVPGMDAAAFKQAAEGAGQNCPVSSAMKGNVTHNVTAELA"
     gene            complement(3819..4742)
                     /locus_tag="VFV93_12930"
     CDS             complement(3819..4742)
                     /locus_tag="VFV93_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5166298.1"
                     /translation="MAITFGMHVGQQNISVAELRRLWTLADTKGFGWLSVWDHFYCAT
                     SDEDPHLEAVALMGALAAETKNIRFGCMVFAAPFRNLGLLAKSMITIDHLSGGRVEFG
                     LGAGWHEPEFRAFHYDLLGVKQRLDRVEEAMVVMRSFLDNEVTDHHGEYFDFTNAYMN
                     PRPLGKLPLWIGGLGEQRTARMAARYADGWNIPYVGPGAFQHKVQVLHDWCEKEGRDP
                     AKVQTATQLGFFMAASDDPAVMAAAEAEMKRKTPHQTPSGQLAGSPAQVIERLKKFEA
                     AGATNINIAFRPPVDWEALDVFIRDVMPEFE"
     gene            4893..5996
                     /locus_tag="VFV93_12935"
     CDS             4893..5996
                     /locus_tag="VFV93_12935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5166299.1"
                     /translation="MDLATHNQQDTTTEAGLFAVCRALLAAGTVEDLVEIAPRVIAEH
                     LPLAAGYQVVLIDPVSLNVTPETGDLHQVCIPMQYGNRLVGGIVIDAGTDGVVLTDAT
                     QRALEQIAELLATAIVTRQRIADEQRSLLDEAAELKLDIVSMLSHEMRTPLASIKGYA
                     TALLLDDVEWDDASRVEFLTAIDEESDHLARLITDMLDSAAIEAGEITIVPEPILVPR
                     IVKRVVDNMQIRSDTHRFIVIFPDEFPVIEADAQRIEQVLTNLLDNAMKYSPNGGLIV
                     VRGEVSEREVIISVSDQGIGIAPEHLNKLFERFFRARSSAGPQVSGTGLGLPISDAIV
                     RAFGGRIWAESTLGSGTTLSFTIPRDRSDGADG"
     gene            5989..6702
                     /locus_tag="VFV93_12940"
     CDS             5989..6702
                     /locus_tag="VFV93_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166300.1"
                     /translation="MVESAGQALGARILVVEDEARLVRLIRAVLETQGYRVTVVPTGE
                     RALAQVALEMPDLVLLDLLLPGGMDGFEICRRIREFSMVPVVMLTASAREEEKLAGFD
                     AGADDYITQPFSARELLARIQAVLRRTQVKQDSPAVIEIGELSINLASQRVSVAGEPV
                     HLTPTEYRLLLALARQPDRVMTHTALLTDVWGIEYRDEVDYLRTYIRYLRQKLEPDPA
                     NPRYLITRPGVGYSLSTEG"
     gene            6897..>7294
                     /locus_tag="VFV93_12945"
     CDS             6897..>7294
                     /locus_tag="VFV93_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX5166301.1"
                     /translation="MQETQPRIDVDKESQGSHLLRDAIAHVRAAAKRLGLNDGLLQVL
                     ETPEREINVALPVEMDNGTIRVFHGYRVQHSRLLGPAKGGFRYHPNVDIDEVRGLASL
                     MTWKCSLLNLPYGGAKGGVSVDPTVLSTSEL"
BASE COUNT         1232 a         2328 c         2479 g         1255 t
ORIGIN      
        1 ccagaggtag gtcgcgacct tccagcccca cggcttcggg tggtggacgt cgtagacaac
       61 ggtcgcctcg gctggtccgg acaggcgggc gtcgccggtg acagcctcct gcgggcggcc
      121 gtcttcgcgc agttcgcccc acaggtagct gttggaccag gtcagcgcct ccggagtcag
      181 gctggcgtcg tcggctccga tgtagaagac cttcggctgg gtaccctgct ccggcttgcg
      241 aacgctgacc tgctcgcgcg agatcagcgc gtggatgtcg ctggtcgggt cgtcgatgtc
      301 gccggcgatg attgccttgg ccgggcagac gacgacgcag gccggcagca gcccgacatc
      361 gacgcggtgc gcgcagtagt tgcacttggc tgccgtgttg gtgaacgggt cgatgtagat
      421 tgcgtcgtac gggcaggcct gcatgcacga cttgcagccg atacagcggg aagggtcgaa
      481 atcgacgatc ccgtccgggc ggcggaagag cgccttcgtt gggcagatct cgacgcaggg
      541 cgcgttgtcg cagtggttgc agcgttcgac ggtaaagaaa cggcgcgagt tggggaattg
      601 ccccttctcg gtgtacttca cccaggtgcg gaagacgcca accggaacct cgttctccga
      661 cttacaggcc accgtgcagg catggcagcc aatgcagcgc cggtggtcga tcacgaatcc
      721 gtagcgtgac acgcggccct cacccccacc cggctcaatc tcccgccggc aggcgagagc
      781 gagcctggta catgcgtcgt ccgtcgatct tcgcgcacgg gcaagctgat tcgagatgga
      841 cgatgcgcga ccccgaggcg cagtgtaaca gcgattgggg agatgcgcgc ggcgggctag
      901 aacgcgccca gcgccaggtt gaggaggatc gtcagcagga cggagacgac gatcgagagg
      961 atcaggcagc ccggtgagca gccccagacc tggattcgcc cgcccttgta gcttcgtggt
     1021 ccgaacagat cgcgcatggt cagttggctc ctatgcaatc acgacgttgc ccccgctgcc
     1081 tgcccgcgag gcaaacggcg tgccagtgtc ggggtcgccc ttgacgcgtc cggctgcgac
     1141 tcctaggctt cgggcggcga ttcgagcgtc tacaccatag agggaacgat ccacgatgat
     1201 caatgacagc ggcgttgtgc agcccccgac gatgtacgag caggtattcg ggactccggc
     1261 tgaacgccgc gaacgtgcgc gaaagcggtt gtcgattgtc cgctggtttg cgctgctcga
     1321 tctgatcctg atcgtcacgt tgacggcggc gtcgttcgcc ggtaatcgcg agctggtcgg
     1381 catcatcggg ccgatccacg gcggcaactt cgtcctgctg ctggtgctgg ttgggacggc
     1441 ggctgccgac ggtctctgga gctggtggtt cccggccgcc gtcctgctga cgggcgggcc
     1501 ggttggcgcg ctgatcggtg agcggctcgt cagccggcga ttggcgcggg actgatggat
     1561 acgaccctca cctggctcgt tcggtacggg cacgtcctct tcgcggccgg ctggcttggt
     1621 ggctacgccg ccctggcgct ggcgattgtc ccactcctgc agcgcgagcg aactgccgga
     1681 ctggcccggc tggctgtcgg cacggccagg acgttgacct acattggcgc cgtgacgatg
     1741 ttcttcggcg tctttctgat cacccgtaca cgcgggctgg acaacctgat cggcggcgag
     1801 tggggcacga tcgttatcag ctgcatcgtg atcgcgatcg ccttggccgg catcggcgat
     1861 ggcccgttac gccagagcct ggttcacatg atcgacaccg gcgacggcac tgccgcgcgc
     1921 cgctgggcgc tggtcggctt cggactggcg gttctcgcga tcggcctgat gacgcgggcc
     1981 gtctacgcgt cgtcgtaacg gacggagggg cgcgcatgtc aaccagcgag tcggagattc
     2041 tgcgcgagca gcaggagtat tacaacgcgc gggccgctga gtacgacgag tggtggatgc
     2101 gggtcggccg ctacgaccgt ggcgccgagg agaacagcat ctggttcgcc gaacaggagg
     2161 aagtgcgctc agcgttcgac acgctcgact ggggtggcga gatccttgag ctggccggtg
     2221 ggacgggcat ctggaccgag tggctcgcct cacgtgcgcg gcacatcacc gtcctggatg
     2281 gttcgctgga gatgatcgcg atcaactcgg ttcgcctgga ggcaggcggc taccgcggca
     2341 aggccgacta tcaccaggtc aacctgttcg actggcagcc ggagcggcag tacgacggcc
     2401 tcttcatggg ctacttcatc tcccacgtcc cgaccgccat gatggactcg ttcctcgcga
     2461 cggtcgcgag tgcgctgaag ccgggcggcg tcattggcct gctcgacggc cggcgcgaaa
     2521 cgcgctcgac atcatccgac cagcccccgc cggggccgga atcggacctg acgatgcggc
     2581 ggctgaacga cggccggagg ttccagatcg tcaagcgctt cgacgagcca gccccgctga
     2641 cggccgcgtt ggcccgtcaa gggatcgacg cggatgtgcg aacgacggcg atccacttcc
     2701 tctacgcaac cggccacaag cagtagccat ccccctgtcg tcctgagccg cagcgaagga
     2761 cgacagggct attggccgcc cggcccgtca cgatcacggt agtgaacggc tcagcgtcgc
     2821 gtgtcaggtg tcgggcgaat cagtggcggg gtcgccggtt tgcggcgccc tggaccgctg
     2881 cggagggcgt cgatgacgat caggaagacg ctttgtggtg aggcggtctc ggccttgctc
     2941 cctgggtgct ggcggttacg gtcaatcgcc gcgccgcagg tcggacacgt cttgctggca
     3001 tttgagattg cgccgttgca ttgcgggcaa acgagcaggc tgagcatcgg tgggcggcag
     3061 tcccctctca cgaccgcctc caagcatgcc tgagagtcac gaccggcgac agacccgaaa
     3121 gtcatagcca gatcaggaca tccgcatatc agttgccggt ttgccaatac acatggtgca
     3181 gtccgttgcg caagcagcgt cagttccgcg catgaacgtt gccccccggc gctgactccg
     3241 gtaggctgac ggctggtacg cacgtcagcg gaaccggaag caggagggat tcccatggca
     3301 ggaacgacca agaccgcgaa cgtcacctgg agcggtagcc tgatggaggg caagggcacg
     3361 atcgatagcg tcggcagtgg cgcgatcagc aatctcggcg tgacctggaa gaaccgcgcc
     3421 gaggccgccg aaggtaccag ccccgaggag ctgatcgccg ccgccaacgc cgcctgcttc
     3481 gcgatggcgc tctcagccgg cctggccgga gccggtcacg cgccggagaa gctcgaagtg
     3541 accgccaagt gcacgttctc gctcgacaac ggccccaaga tcacgacgat ggacctgcac
     3601 gtgcgcggca ccgtccccgg catggacgcc gccgccttca agcaggccgc cgaaggcgcc
     3661 ggccagaact gccccgtctc gtcagccatg aagggcaacg tgacgcataa cgtgacggcg
     3721 gaattggcgt agatcgggat tcgggattcg gggctgggat acatcggatg tctacgagga
     3781 acatgctgtg tgtcccatcc ccgaatcccg aatcccgatc actcaaactc cggcatcaca
     3841 tcccgaatga acacatccag cgcctcccag tcgactgggg gacggaaggc gatgttgatg
     3901 ttggtcgcgc cggcggcctc gaacttcttc aggcgctcga tgacctgggc ggggctgccg
     3961 gcgagttggc cggatggggt ctggtgcggc gtcttgcgct tcatctcggc ctcagcggcg
     4021 gccatgacag ccgggtcgtc ggacgcggcc atgaagaagc cgagctgggt agcggtctgg
     4081 actttggctg ggtcgcggcc ctccttctcg caccagtcgt gcaggacctg gaccttgtgc
     4141 tggaacgctc cgggaccgac atacgggatg ttccagccgt cggcgtacct ggccgccatc
     4201 cgggcggtgc gctgctcgcc gaggccgccg atccagagcg gaagcttgcc gagcggacgc
     4261 gggttcatgt aggcgttggt gaagtcgaag tactcgccgt ggtggtcggt gacctcgttg
     4321 tcgaggaacg agcgcatgac gaccatcgcc tcctcgaccc ggtcgagccg ctgcttgacg
     4381 cccagcaggt cgtagtggaa ggcgcggaac tccggctcgt gccagccggc gccgaggccg
     4441 aactcgaccc gcccgcccga gagatggtcg atcgtgatca tcgacttcgc cagcagcccc
     4501 aggttgcgaa acggcgctgc gaagaccatg cagccgaagc ggatgttttt cgtctcagcc
     4561 gccagcgccc ccatcagcgc gaccgcttcg aggtgcggat cctcgtcact tgtcgcgcag
     4621 tagaagtggt cccagaccga gagccagccg aagcctttcg tgtcggccag tgtccagagc
     4681 cgccgtagct cggcgacgct gatgttctgc tgcccgacat gcattccgaa ggtaatagcc
     4741 atccgtcccc ttttctgccg gccgcgttcc ggctcgtggt gtggaaaccg cgcctgttcg
     4801 gcagtatcgc acgctccatt gcagacggcg cacggcgcag ccggtaagat tcgctggttg
     4861 cgggccacag ccgggacaag ctggatgggg tgatggatct ggcaacgcac aatcagcagg
     4921 acacaacgac cgaggcgggg ctcttcgcgg tctgtcgcgc gctgctggcg gccgggacgg
     4981 tcgaggatct ggtcgagatc gcgccgcgcg tcatcgccga gcacctgccg ctggcagccg
     5041 gctatcaggt cgtcctgatc gaccccgtca gcctgaacgt cacgccggag accggcgatc
     5101 tgcaccaggt ctgcatcccg atgcagtatg gcaaccggct ggtcggcggg atcgtgatcg
     5161 acgccgggac tgacggagtc gtcctgaccg atgcaaccca gcgggcattg gaacagattg
     5221 ccgagttgct ggcgacggcg atcgtcaccc gccagcggat cgccgacgag cagcgcagcc
     5281 tgctcgacga ggcggccgag ctgaagctcg acatcgtctc gatgctctcg cacgagatgc
     5341 ggacaccgct ggcctcgatc aagggctacg cgacggcgct gctgctcgac gatgtcgagt
     5401 gggacgacgc cagccgggtc gagttcctga cggcgattga cgaggagagt gaccacctcg
     5461 cgcggctgat caccgacatg ctcgattcgg ccgccatcga ggccggcgag atcacgatcg
     5521 ttccggagcc gattctcgtt ccccggatcg tcaagcgggt cgtcgacaac atgcagatcc
     5581 gctccgacac acaccgcttc atcgtgatct tccccgatga attcccggtc atcgaggctg
     5641 atgcccagcg gatcgagcag gtactgacca acctgctcga caacgcgatg aaatattcac
     5701 ccaacggcgg gctgatcgtc gtccgtggcg aggtgagcga gcgcgaggtc atcatcagcg
     5761 tcagcgatca ggggatcggg atcgcgccgg agcatctcaa caagctcttc gagcgcttct
     5821 tccgggccag atcgagcgcc gggccgcagg tctcgggaac cggcctcggc ctgccgatca
     5881 gcgacgcgat cgtccgcgcc ttcggcggtc ggatctgggc cgagagcacg ctcgggagtg
     5941 ggacgacgct ctcgttcacg attccacgcg acaggagtga tggcgccgat ggttgaatcg
     6001 gcagggcagg cgctgggcgc caggattctg gtcgtcgagg atgaggcccg gctggtgcgg
     6061 ttgattcggg ctgttctcga gacgcagggc taccgcgtca cggtcgtccc gaccggcgag
     6121 cgggcgctgg cgcaggtcgc gctggagatg cccgacctgg tcctgctgga tctgctgctg
     6181 ccgggcggga tggatggctt cgagatctgt cgccgcatcc gcgagttctc gatggtcccg
     6241 gtcgtcatgc tgacggccag cgcccgcgag gaggagaagc tggccggctt cgacgccggc
     6301 gcggacgact atatcaccca gccgttcagc gcccgcgagc tgctggcgcg catccaggcc
     6361 gtcctgcgtc ggacgcaggt caagcaggac agcccggccg tgatagagat tggcgaactg
     6421 tcgatcaacc tcgcctcgca gcgcgtctcg gttgccggcg agccggtcca cctgacaccg
     6481 accgaatacc ggctgctgct ggcgctcgcc cgccagccgg accgcgtcat gacccacacg
     6541 gcgctgctga ccgatgtctg gggtattgag taccgcgacg aggtcgacta cctgcgcacc
     6601 tacatccgct acctgcgcca gaagctcgag cccgaccccg ccaacccccg ctacctcatc
     6661 acccgccccg gcgtcggcta cagcctcagt accgaggggt gaggcgtacg gtcttgcgaa
     6721 cgctgttcgg ctgggcgggt gtccgtttgc gctgattcct cgatatttct catgacattc
     6781 tcatacctcc cgcatccgct ctcatccggc tctcacggcc ggtcaggcag gattgagagt
     6841 gcaaaccgaa acgtgcgccg ttgcacgtca cgcatggaag atgagtgagc gaacagatgc
     6901 aggaaacaca gccgaggatc gacgttgaca aggaatcgca gggttcgcac ctgctgcgtg
     6961 atgcgatcgc gcatgtgcgg gcggcggcga agcggctggg cctgaatgac gggctgctgc
     7021 aggtccttga gacgccggag cgggagatca acgtcgccct gccggtcgag atggacaacg
     7081 ggacgatccg cgtcttccac ggctatcgcg tacagcattc gcgcctgctg ggtccggcca
     7141 agggcggctt ccgctatcac ccgaacgttg atatcgacga ggtgcgcggc ctcgccagcc
     7201 tgatgacctg gaagtgctcg ctgctcaacc tgccgtacgg cggcgccaag ggtggcgtct
     7261 cggtcgatcc aactgtgctg tccaccagcg agct
//
LOCUS       DASUGJ010000570         3253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1621707, whole genome shotgun sequence.
ACCESSION   DASUGJ010000570 DASUGJ010000000
VERSION     DASUGJ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621707"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1728)
                     /locus_tag="VFV93_12950"
     CDS             complement(250..1728)
                     /locus_tag="VFV93_12950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5166302.1"
                     /translation="MPIRLRITLATTFLMAIALAALGTGMYITTSSSLHNDMDNRLRR
                     VYEGFQENPGFWRTENGEIRELIINPEPFASGGTFLQIRNATGSQVVLSENLNDKFIP
                     VSQDVLQRNEGFDTVYYDTVIDGAYLRVYSGRLNDPRGNLAAYVQVAEPLQPVNATLS
                     TLKRNIIWGSVFSTLMLACGAWLVGDAAMRPLAKMSNTARSIGRTGDLSTRLHPPKTR
                     DEAQHLGETFNEMLSRLEETFNAQRRFVADASHELRTPLTALRANADIMLRQIEGGIY
                     DREDLAEGLSDIRDEVDRMTRLVQHLLTLARADVGWRPELEVIDLVDIARDAARVASP
                     LVRGQQFALDMPPPDRDGSSPEVDVRGNADQLRQLILILLDNAFTYAPADSDVRLAVR
                     QDDDVAVLSVHDSGPGIAPDHLRRIFERFFRTDDARARSSGGTGLGLAIARWIVTVHD
                     GEITATSGSETGTTFEVRLPRAREDAKASRTSSEVSRVAPAG"
     gene            complement(1744..2454)
                     /locus_tag="VFV93_12955"
     CDS             complement(1744..2454)
                     /locus_tag="VFV93_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166303.1"
                     /translation="MTGTLSRPARILVVEDERSILSAIRRGMAFEGYAVEVAETGNQA
                     LDIVRDRLPDLIVLDIMLPGIDGLEVCRRIRAAGEDVPILMLTARDEVQDRVAGLDAG
                     ADDYLIKPFAFEELLARVRALLRRLERHDDDRLTGEELRYGDLTLDTTTRYAHRGQRR
                     IELTTREFDLLMLFMRHPNQVLPREMIMERVWGYDFPGESNVLEVYVKNLRRLLEAGG
                     EPRVLQTVRGAGYVLRRD"
     gene            complement(2474..>3253)
                     /locus_tag="VFV93_12960"
     CDS             complement(2474..>3253)
                     /locus_tag="VFV93_12960"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5166304.1"
                     /translation="TVGSITSNDLHAFLDRHYRPERMVIAVAGKVDHDHVVELAERYF
                     GDLPAGEGEHFVAAEHRQSAPKVRLVTRPTEQAHLCIGHPAIPYTDERRYSQGMLDAI
                     LSSGMSSRLFQEIREKRGLVYSVYGYFRQYADAGQGVVYAGTDLQRVDETIDAILTEL
                     EKLRSEAVPEDELQRTKELRKGRILMGMEDSRSVAGWIGSQELTFGEILTPDEVMERI
                     DAVQSDEMLELARELIREEHLSLALVGPYEDAEHFRSQLKF"
BASE COUNT          555 a         1009 c         1088 g          601 t
ORIGIN      
        1 ggatgactgc cgccgaggtt ctggccgacc ccgcctgtcc gccgtggatg ccgccaagcc
       61 tcgcgctggt cgaacgcaaa cggcaggcgc tgggctggta ggctcaccat cccgcaattc
      121 aacgatccat cctcgatccg gtacgggcga tatcgcaata tcccccttca gccacctggc
      181 gcgcggagca gggcgatatt gcgatatcgc ccgtaccgga ttgaccattg ggattgaaac
      241 tggaggccct cagcccgccg gcgcgacccg cgagacttcg ctggacgtgc gtgaagcttt
      301 cgcgtcctcc cgcgcgcgag gcaggcgcac ctcgaaggtc gtgcccgttt cggagccgct
      361 cgtggcggtg atctcgccgt cgtggacggt gacgatccag cgtgcgatgg cgaggccgag
      421 gccggtcccg ccggatgaac gggcgcgggc gtcgtcggtg cggaagaagc gctcgaagat
      481 gcggcgcaag tggtccggcg cgatgccggg accgctatcg tggacgctca gcacggcaac
      541 gtcatcgtcc tgccggacgg ccagccggac atcgctgtcg gccggagcat aggtgaaggc
      601 gttgtcgagc aggatcaaga tgagttgacg taactgatcg gcgttgccgc gcacatcgac
      661 ctctggggaa ctgccgtcgc ggtccggcgg cggcatgtcc aacgcgaact gctggccgcg
      721 cacgagcggc gaggcgacgc gggcagcatc gcgggcgatg tcgacgaggt cgatgacttc
      781 gagttcgggc cgccagccga cgtcggcgcg ggccagtgtc agcaggtgct ggacgagccg
      841 cgtcatccga tcgacctcgt cgcggatatc ggagagtcct tcggccagat cctcgcggtc
      901 gtagatacca ccttcgatct gacgcagcat gatatccgcg ttggcgcgca gggccgtcag
      961 cggcgtccgt agttcgtggg aggcgtcggc gacgaagcgg cgttgggcgt tgaacgtctc
     1021 ctccagccgc gagagcatct cgttgaacgt ctcgccgagg tgttgtgcct cgtcgcgggt
     1081 cttggggggg tgcaggcgcg tcgagagatc gccggtccgc ccgatcgagc gggccgtgtt
     1141 gctcatcttc gccagcggcc gcatggcagc atcgccgacc agccaggcgc cacaggccag
     1201 catcaacgtc gagaagacag agccccagat gatgttgcgc ttcaacgtgc tgagtgtcgc
     1261 gttgacgggt tgtagcggtt ccgcgacctg aacgtatgca gcaagatttc ctcgtggatc
     1321 gttcaggcgc ccggaataga cccgcaaata ggcgccgtcg atcactgtgt catagtagac
     1381 agtgtcgaaa ccctcattac gctgcaacac atcttggctg acagggatga acttgtcgtt
     1441 cagattctcg gaaaggacga cctggctccc ggtcgcgttt ctgatttgaa gaaacgtccc
     1501 cccagacgcg aacggttcgg gatttatgat cagttctcga atttcaccat tttcggtgcg
     1561 ccagaaccct gggttctcct gaaatccctc gtagaccctt cggaggcggt tgtccatgtc
     1621 gttgtgcagt gaactggatg tggtgatgta catgccggtg ccgagcgccg ccagcgcgat
     1681 ggccatcagg aacgtcgtgg cgagagtgat acgcaggcga attggcatgc tctgtccggg
     1741 ttgctagtcg cggcgcagga cgtagccggc gccacgaacg gtttgcagaa cgcgtggctc
     1801 gccgccggct tccagcagcc ggcgcaggtt cttgacatac acttcaagga cgttcgactc
     1861 gccggggaaa tcgtaccccc agacgcgctc catgatcatc tcacgcggca gcacctgatt
     1921 cggatggcgc atgaacagca tcagcaggtc aaactcgcgc gtggtcagct cgatccggcg
     1981 ctggccccga tgggcgtagc gggtggttgt gtcgagcgtc aggtccccgt agcgcagctc
     2041 ctcgccggtc agccggtcgt catcgtgccg ttcgaggcgg cgcagtaacg cccgcactcg
     2101 cgccagcagc tcctcgaagg cgaacggttt gatcaggtag tcgtcagcgc cggcgtcgag
     2161 accggcgacg cgatcctgga cctcatcgcg ggccgtcagc atcaggatcg ggacatcctc
     2221 gccggcggcc cggattcgcc ggcagacttc gaggccgtcg atgccgggca gcatgatatc
     2281 gagcacgatc aggtccggta gtcggtcgcg aacgatgtcg agcgcctggt taccagtctc
     2341 agccacttcg acggcgtagc cctcgaaggc catcccgcgt cggatggccg agaggatgct
     2401 acgttcgtcc tcgacgacaa ggatgcgtgc cggacggctg agtgtgccgg tcatcgcgcg
     2461 atcccgtcac ccatcagaac ttgagttgcg agcggaagtg ctcggcgtct tcatacggac
     2521 cgaccagcgc cagtgagagg tgctcttcgc ggatcagctc acgggcaagc tccagcatct
     2581 catcggactg gacggcgtcg atccgctcca tcacctcatc cggtgtcagg atctcgccga
     2641 acgtcagttc ctgcgagccg atccagccgg ccactgagcg ggaatcctcc atccccatca
     2701 ggatgcggcc tttgcggagt tccttggtgc gctgtagttc gtcctcagga accgcctcgc
     2761 tgcgcagctt ctcgagttcg gtcaggattg cgtcgatcgt ctcgtcgacg cgttggaggt
     2821 cggtgccggc gtagacgaca ccctgaccgg cgtccgcgta ctggcggaag tagccataga
     2881 cggagtagac caggccgcgc ttttcgcgaa tctcctggaa gaggcgcgag ctcatcccac
     2941 tggagaggat cgcgtcgagc atgccctgcg agtatcgacg ttcgtcggtg tacggaatcg
     3001 caggatggcc gatgcagaga tgcgcctgct cggtcgggcg ggtgaccagc cggacttttg
     3061 gggcgctctg ccgatgctcg gcggcgacga agtgctcacc ctcgccggcc ggcagatcgc
     3121 cgaagtagcg ctcggccagc tcgacgacgt ggtcgtgatc gaccttgccg gccacggcga
     3181 tcaccattcg ctcgggccgg tagtgtcggt cgaggaaggc gtgcaggtcg tttgacgtga
     3241 tgctgccgac cgt
//
LOCUS       DASUGJ010000571         5458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1481398, whole genome shotgun sequence.
ACCESSION   DASUGJ010000571 DASUGJ010000000
VERSION     DASUGJ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481398"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFV93_12965"
     CDS             complement(<1..471)
                     /locus_tag="VFV93_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166305.1"
                     /translation="MAEPPSKDSHVTWRGSSGILRVLLIGLCSGALYLAVYLLQRGIY
                     LNGLASELGQPADRGRLALQAALYVGCTIALFALYGWLLMLCHGGQLSDGRVRRLALA
                     LPVLFNLGLLFGRPYLSTDLFTYLAHGKLSLLDAGNPYVHQPTEVEQLPFGLDLL"
     gene            complement(514..1818)
                     /gene="eno"
                     /locus_tag="VFV93_12970"
     CDS             complement(514..1818)
                     /gene="eno"
                     /locus_tag="VFV93_12970"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5166306.1"
                     /translation="MNWSGSAIEAIWSREILDSRGNPTVEVDVYLEDGSFGRAAVPSG
                     ASTGAFEAVELRDGDSGRYGGKGVLNAVENVNETIGPELEGYDAADQRAIDLRMIELD
                     GTDNKGNLGANAILGVSLAVARAAADAAGLPLYKYLGGPGAHVMPVPMLNILNGGKHA
                     QGSSVDFQEFMVMPVGAESFSECLRWGAEIFHALKGVLAQNGYSTMVGDEGGYAPSLG
                     SNAEALDLIAEAINRAGYALGSDVLLAMDPASTEFFEDGNYVLKGEGRTLSAGEMIDW
                     YADATSRFPIISIEDGLAEDDWNGWKALTARIGDKVQLVGDDLFVTNTERLARGIRES
                     AGNSILVKLNQIGTLTETFEAVNLAHRSGFTAVISHRSGETSDSFVADLVVATNAGQI
                     KTGAPSRMDRVEKYNQLLRIEEQLGEQAIFAGKEAFGQRLVS"
     gene            complement(1906..2265)
                     /locus_tag="VFV93_12975"
     CDS             complement(1906..2265)
                     /locus_tag="VFV93_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166307.1"
                     /translation="MFDRQFFAAILPEHVRAEVAANPGKVPIIELFLLSGAVLDLCHV
                     VRLADAWVAVAHFCHEDRYDDTDIAFLPYGTITRVSLTMRDAKEREIGFSTSHTPIET
                     PNGMPVQPALPAGHSPS"
     gene            complement(2308..2667)
                     /locus_tag="VFV93_12980"
     CDS             complement(2308..2667)
                     /locus_tag="VFV93_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166308.1"
                     /translation="MAAVSIDGDQLVVRLSGWESVWALRRKIVVPLRSVLGASAGTVH
                     SKLPRGIRLPGTFVPKLITAGSYWRKSEGWSFWSVRDERKAIDIRLRDTRFVRIVVQV
                     DDPYATVAMIEAATRGL"
     gene            3130..4164
                     /gene="pheS"
                     /locus_tag="VFV93_12985"
     CDS             3130..4164
                     /gene="pheS"
                     /locus_tag="VFV93_12985"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5166309.1"
                     /translation="MNDDLQREALETLKATTNEEELAAWYRDVLGRKGTLSEAMRTLG
                     TLAEDKRRERGRQLNEIKQQLEAAFTARQDEVRAAALNARIGSESVDVTLPGRRQPVG
                     NLHPITAMIREACDIFAHLGFQTVEGPEVEYALYSFDMLNIPQDHPARDVWDTIFINS
                     NEKEITLRPHTSPMQIRTMLKQDPPVRVVVPGRTYRYEAQDATHEWHFHQIEGLAVDR
                     HITMADLRGTLAEFARQIFGGERKVRFRCDYFPFVEPGVDMSIDCPFCKGRGCRTCKG
                     SGWIEILGAGMVHPNVLRNVGYDPEVYSGWAFGMGVDRLVMLRYNVQDLRSFYSNDLR
                     FLAQFDRARL"
     gene            4272..>5458
                     /gene="pheT"
                     /locus_tag="VFV93_12990"
     CDS             4272..>5458
                     /gene="pheT"
                     /locus_tag="VFV93_12990"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5166310.1"
                     /translation="MLVPMKWLKELVPNDLSTEEIGRRLTLAGLEAESITRIGEEWEN
                     VYVALVERVERHPDADRLVLATVAAGEHRLTVVTGAPNIAEGQRVALALAGARLVDPY
                     VEDLQYKTLKPSVIRGVKSEGMVCSEKELGLSGEHEGIMVLPDDAPVGAPLREYLGDD
                     VIEFEITPNLVHAFSVHGIARELAAILASEVKPIERADLSGIDRRDTTVVIEDPNLCG
                     RYALALIDNVTIGPSPDWMQRRLTAAGMRPVNNIVDITNYVMAELGQPMHPFDAGRMA
                     SETVIVRPARPGERIETIDHVQRVLDEQVLVIADETRAIGLAGVMGGVDTEISDDTTR
                     VLLESASFDAKSIRRTARALKLPSEASARFQRGVDPNLAWPAIERFVALLREIDPRAT
                     IRYV"
BASE COUNT          996 a         1747 c         1786 g          929 t
ORIGIN      
        1 gagcaggtcg aggccaaacg gaagctgttc aacctcggtc ggttgatgca cgtatgggtt
       61 gccggcatcc agcaagctga gcttgccgtg ggcgagatag gtgaacaggt cggtcgatag
      121 gtatggccgg ccaaacaaca aacccaggtt aaacaggacg ggcaacgcga gcgccagccg
      181 ccgcacccga ccatcgctga gttgcccccc atgacacagc atcaacaacc agccgtagag
      241 cgcgaacagc gcgatcgtgc agccgacgta gagcgcggcc tgtagcgcca gccggccacg
      301 atcagcgggc tggcccaact cagaggccag cccgttcagg tagattccgc gttggagaag
      361 gtaaaccgcg aggtagagcg ccccgctaca gagcccgatc agaaggaccc gcaggatccc
      421 gctgctgcca cgccacgtga catgtgaatc ctttgacggt ggctcggcca cgacctggac
      481 tccctccgga tgggtgccca ggtggttgcg ttctcaggac accagtcgct ggccgaatgc
      541 ttccttccca gcgaagattg cctgttcgcc cagttgctct tcaattcgca ggagttgatt
      601 atacttctcg acccggtcca ttcgcgatgg tgctccggtc ttgatttgtc ccgcgttggt
      661 ggcgacgacc aggtcggcca cgaacgaatc ggacgtctca cccgaccggt gcgagatgac
      721 ggccgtgaag ccggaacgat gcgccagatt gacggcctcg aacgtctccg tcagcgtgcc
      781 gatctggttg agcttgacga ggatcgagtt gccggccgac tcgcggatgc cgcgcgccag
      841 ccgctcggtg ttggtgacga acaggtcgtc accaacgagc tggaccttgt cgccgatccg
      901 ggccgttagc gccttccagc cgttccagtc gtcctcggcc agtccgtcct cgatcgagat
      961 gatcgggaag cgggaggtcg cgtcggcgta ccagtcgatc atctcgccgg ccgacagcgt
     1021 ccggccctcg cccttgagga cgtagttgcc atcctcgaag aattcggtcg aggcggggtc
     1081 catcgccagc agaacatcgc tgccgagcgc gtagccggcg cgattgatcg cctcggcgat
     1141 caggtcgagt gcctccgcgt ttgagccaag gctcggcgcg tagccgccct cgtcaccgac
     1201 catcgtcgag tagccgttct gagccaggac gcccttcagc gcgtggaaga tctcggcgcc
     1261 ccagcgcaga cactcgctga atgattcggc gccgaccggc atgaccatga actcctgaaa
     1321 atcgacgctg gagccctgtg cgtgcttgcc gccgttgagg atgttcagca tcgggaccgg
     1381 catgacgtgc gcgcccggcc cgccgaggta cttgtagagc ggcaatcccg ccgcgtcggc
     1441 ggcggcccga gcgacggcca gcgagacgcc gaggatcgcg ttcgcgccga ggttgccctt
     1501 gttgtcggtc ccgtccagtt cgatcatccg caggtcgatc gcgcgctggt cggccgcgtc
     1561 gtagccctcc agctccggcc cgatcgtctc gttgacgttc tcgaccgcat tgaggacgcc
     1621 cttgccgccg tagcggcccg agtcgccgtc gcgcaactcg acggcctcga aggcgccggt
     1681 cgacgctccg gagggaacgg cggccctgcc gaatgagcca tcttcgagat agacgtccac
     1741 ttccactgtt gggttgccac gcgagtcgag aatttcgcgc gaccagatcg cctcaatcgc
     1801 cgacccgctc cagttcatcg ccatcagcgt ccctccccga tcaatccgtc aactccgcca
     1861 gttcgcggat gtcactgtcg aatcgtaccc gctggcaacc ggctgtcacg atggcgaatg
     1921 gccggccgga agcgccggct gcaccggcat gccgttcggt gtctcgatcg gagtgtgcga
     1981 ggtcgagaag ccgatctcgc gctccttcgc gtcgcgcatc gtcagcgaga cgcgggtgat
     2041 cgttccgtac ggcaggaagg cgatgtccgt gtcatcgtag cggtcctcgt ggcagaagtg
     2101 cgcgacggcg acccaggcat cggccagccg gacgacgtga cacaggtcca ggacagcgcc
     2161 actaagcagg aacagctcga tgatgggcac tttcccggga ttggccgcca cttctgcccg
     2221 gacgtgctcc ggaaggattg cggcgaaaaa ttgccggtca aacatctgtc ggttttcctc
     2281 gcacttctga cgaacgagcc tcccggctca caaacccctt gtcgctgcct caatcatggc
     2341 taccgtcgcg tatggatcat caacctggac cacgatgcga acgaagcgcg tatcgcgcag
     2401 gcggatatca atcgccttcc tctcgtcgcg aacgctccag aacgaccagc cttccgattt
     2461 gcgccagtag ctgccggccg tgatgagctt ggggacgaag gttcccggca agcggatgcc
     2521 gcgtggcaac ttagagtgca cggtcccagc gctcgcgccc aggacactcc gcagtgggac
     2581 gacgatcttg cgacgcagtg cccagaccga ctcccagccg ctgagccgga cgaccagctg
     2641 gtcaccatcg atagacaccg ctgccatttg ttcgcccttc ctgcaacgca ttgcctattc
     2701 cggcagaaac gtacaccaga cgggcatccc gctgccgcga tcaatgggcc gaatccgctg
     2761 gtatactgcg atctgaagat gaatcgtggc gatgatggag acgggtcgcg acgacggacg
     2821 ccgacaggga cgccaagcca gagactgaga gccggcgcgg cgggccagcg cgacattcac
     2881 tccggagccg ggcggtgaac gcggctgcca gtggcagacg cctgtagtcg cacccgggag
     2941 cttcccgtta caggagcgcg ttcggatcgt tccgaacgac tgaggccggc atgtcagtac
     3001 gcctgcaccg gcgaatcagg gtggtaccgc gtgagcactc ctcgcccctg agcgaggggt
     3061 gtttttgttg tccgtcgcct gcatcagacc gagaacatcg acaggcggac gcgggaggga
     3121 gaaacgacga tgaacgacga cttgcagcgc gaggcgctgg agactttgaa ggccacaacc
     3181 aacgaggagg aactggcggc ctggtatcgc gacgtcctcg gccggaaggg cacgctctcc
     3241 gaagcgatgc ggacactcgg cacgctggcg gaggacaagc gccgcgagcg gggccgccag
     3301 ctgaatgaga tcaagcaaca gctcgaagct gccttcaccg cccgccagga tgaggtccga
     3361 gcggcggcat tgaatgcgcg gattggttcc gagtcggtcg atgtcacgct gcccggacga
     3421 cgccagcccg tcggcaacct gcacccgatc acggcgatga tccgtgaggc atgcgatatt
     3481 ttcgcgcatc tgggcttcca gacggttgag gggccggagg tcgagtacgc gctctacagc
     3541 ttcgacatgc tcaatatccc gcaggaccat ccggcccgcg atgtctggga caccattttc
     3601 atcaactcca atgagaagga gatcacgctc cggccgcata cctcgccgat gcagattcgg
     3661 acgatgctga agcaggaccc gccggttcgt gtcgtcgtgc cgggacggac ctaccgctac
     3721 gaggcgcagg acgcgaccca cgagtggcac ttccaccaga tcgaggggct ggccgtcgac
     3781 cgtcacatca cgatggcaga cctgcgcggg acgctggccg agttcgcgcg ccagatcttc
     3841 ggcggcgagc gcaaggtgcg cttccgttgc gactatttcc cgttcgtcga gccgggcgtc
     3901 gacatgtcga tcgactgccc gttctgcaag gggcgcggtt gccgcacttg caagggcagc
     3961 ggctggatcg agatcctcgg ggccggcatg gtccatccga acgtcctgcg caacgtcggt
     4021 tacgacccgg aggtctactc cggctgggcg ttcgggatgg gcgtcgaccg gctggtcatg
     4081 ctgcgctaca acgtccagga tctgcgctcg ttctattcca acgatttgcg cttcctggcc
     4141 cagttcgacc gggcgaggct gtgacgagtc gtgacgcgtg acgcgtaacg cgtgacgcga
     4201 tgggcttgag tccgccgttc tccgcgtcac gcatgacgcg tcacgaaaca agaacaatca
     4261 ggagcgatcg aatgctagta ccgatgaagt ggttgaagga actggtgccg aacgatctct
     4321 cgaccgaaga gatcggcagg cgtctgacgc tggccggtct ggaagccgag agcatcaccc
     4381 ggatcggcga ggagtgggag aacgtctacg tcgcgctggt cgagcgcgtc gaacgccatc
     4441 cggatgcgga ccggctggtg ctggcgaccg tcgcggccgg cgagcaccgg ctgacggtcg
     4501 tgaccggcgc cccaaatatc gccgagggtc agcgggtggc gctcgctctg gccggggcgc
     4561 ggctggttga tccgtacgtc gaagatctgc agtacaagac gctcaagccg tcggtcatcc
     4621 gcggcgtcaa gtcggagggg atggtctgtt ccgagaagga gctgggactg tcgggtgagc
     4681 acgaggggat catggtcctg ccggatgatg ccccggtcgg cgcgccgctg cgggagtacc
     4741 tcggcgacga cgtgatcgag ttcgagatca cgcccaacct ggtccacgcc ttttcggtcc
     4801 acggtatcgc ccgcgaactg gcggcgatcc tcgccagcga ggtcaagccg atcgagcggg
     4861 ccgacctgtc cggcatcgat cgccgcgata cgaccgtggt catcgaagac ccgaatctct
     4921 gcgggcggta cgcgctggcg ctgatcgaca atgtcacgat cgggccgtcg ccggactgga
     4981 tgcagcggcg gctaacggcg gccgggatgc gaccggtcaa caacatcgtc gacatcacca
     5041 actacgtgat ggccgagctc ggccagccga tgcacccttt cgatgcggga cggatggcga
     5101 gtgagacggt catcgtccga ccggcccgtc ccggcgagcg aatcgagacg attgaccacg
     5161 ttcagcgtgt actcgacgag caggtgctgg tcatcgccga cgaaacgcgg gcgatcggcc
     5221 tggccggcgt gatgggtggc gtcgataccg agatcagcga tgacacgaca agggtcctgc
     5281 tggagtcggc gtcattcgac gcgaagtcga tccggcgaac ggcccgggcg ctcaagctgc
     5341 cgtccgaggc gtcggcccgc ttccagcgcg gcgtcgatcc aaacctggcc tggccggcga
     5401 tcgagcggtt cgtcgcgttg ctgcgggaga tcgatccccg cgcaaccatc cgctacgt
//
LOCUS       DASUGJ010000572         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_787689, whole genome shotgun sequence.
ACCESSION   DASUGJ010000572 DASUGJ010000000
VERSION     DASUGJ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787689"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2221
                     /locus_tag="VFV93_12995"
     CDS             <1..>2221
                     /locus_tag="VFV93_12995"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX5166311.1"
                     /translation="VQTDGPARVGLVAVDRSVFILAENRLNLQQVFAELERLSLQPQA
                     ELHEAEGGIEVQPMFDPWGPTTIPGAEETFRDAGVIVLTNQRVPKGKEIQPDMFRFAA
                     GSSDDDAAAAPVPMAATAANVEESGEAVQNQAQPQRIRQFFPETWIWSDLTTDASGHA
                     SQPVTAPDSITTWMFRAVALSRELGLGIGEAELRVFQSFFVSVDLPFAAIRGEELPAA
                     VALYNYTQAAEEFTVELEPADWFEPLAGLTQTVTVGPNEVGAASFPIRTTALGVHPLR
                     VTARGATVADAIVKEMIVEPEGVAREMLENLVLASGSVREIDLSLPDFTVDGSGRAFL
                     ALTGNVLSQTMDGLESLLQMSFGCGEQNMILFAPNVFITRYLDETGQLKPEVRARAET
                     LMLTGYQREMTYRRSDGSFSAFGESDESGSLWLTGFVLKTFAQAADLVYIDESVLAAG
                     REWIRSHQASDGSFAPVGFVHHTELLGGLDGTTALTAFMAVALHEAGDSDGAATATRY
                     LEGELPGTTDDYPLALLTYALALTGSGQAGAALDALMAIAKEDDDGLHWGDQAAAREP
                     SPYDGFGPGASSAAVETTAYATLALLALDRDLDAGRALHWLAAQRNASGGFGSTQDTV
                     VALQAMTTAAGSNRDDIDATIFISSGDFTHEAHIDAENADILQLIEVPAGGTLTVETT
                     GTGQVMGQAVRRYNQPETGAIERPIFDLSVDYSADEIEVDDILDITATITYNPPEMLG
                     "
BASE COUNT          369 a          810 c          724 g          318 t
ORIGIN      
        1 gtccagaccg atgggccggc gcgggttggg ctggtcgccg tcgatcgctc cgtcttcatc
       61 ctggccgaga accggctcaa cctccagcag gtcttcgccg agctggaacg tctctcgctc
      121 cagccgcagg cggagctcca cgaggcggaa ggcgggatcg aagtccagcc gatgttcgac
      181 ccgtggggcc cgacgacgat ccccggcgcc gaggagacct tccgcgacgc cggcgtgatc
      241 gtcctgacca accagcgggt gccgaagggc aaggagatcc agccggacat gttccgcttc
      301 gctgccggat cttccgacga cgatgctgcc gccgcaccgg tgccgatggc agccacggcg
      361 gccaatgtcg aggaatccgg tgaggccgtt cagaaccagg cccagccaca gcgcatccgc
      421 cagttcttcc cggagacgtg gatctggtcc gacctgacga ccgacgcatc gggccacgcc
      481 agccagccgg tgaccgcgcc cgacagcatc acgacctgga tgttccgggc cgtcgccctc
      541 tcgcgtgagc tgggtctcgg catcggcgag gccgagctgc gcgtcttcca gtcgttcttc
      601 gtcagcgtcg atctgccctt cgccgccatc cgtggcgagg agctgccggc ggctgttgcc
      661 ctctataact acacgcaggc cgccgaggag ttcacggtcg agctggagcc ggccgactgg
      721 ttcgagccgc tcgctggcct gacccagacc gtcacggtcg gcccgaacga ggtcggtgcg
      781 gcctccttcc cgatccggac aacggcgctc ggcgtccacc cgttgcgggt gacggcccgt
      841 ggcgcaacgg tcgcggacgc gatcgtgaag gagatgatcg tcgagccgga gggtgtcgcc
      901 cgcgagatgc tggagaacct cgtcctggcc agcggcagcg tccgcgagat cgacctgagc
      961 ctgcccgact tcaccgtcga tggctcgggc cgcgccttcc tcgcgctgac cggcaacgtc
     1021 ctcagtcaga cgatggacgg gctggaatcg ctgctacaga tgtcgttcgg ctgcggcgag
     1081 cagaacatga tcctcttcgc gccgaacgtc ttcatcaccc gctacctgga cgagaccggc
     1141 cagctcaagc cggaggttcg cgcccgggcc gagacgctga tgctgaccgg ctaccagcgc
     1201 gagatgacct accgccgctc ggacggcagc ttctcggcct tcggcgagag cgacgagtcc
     1261 ggcagtctct ggctgaccgg gttcgtcctc aagaccttcg cccaggcggc cgacctggtc
     1321 tacatcgacg aatcggtcct ggccgccggc cgcgagtgga tccgcagcca tcaggccagt
     1381 gatggctcgt tcgcgccggt cggcttcgtc caccacaccg agctgctcgg cgggctggac
     1441 ggcacgacgg cgctgaccgc cttcatggcc gtcgccctcc acgaggccgg cgacagcgac
     1501 ggagcagcaa ccgccacccg ctacctcgaa ggggagctac ctggcacgac cgacgactac
     1561 ccgctggcac tgctcaccta cgcgctggcg ctgaccggca gcggccaggc cggcgcagca
     1621 ctcgacgcgc tgatggcgat cgcgaaggaa gacgatgacg gactccactg gggcgatcag
     1681 gcggcggccc gtgagcccag tccgtatgac ggcttcggcc ccggcgccag cagcgccgcc
     1741 gtcgagacga ccgcctacgc gacactggcg ctgctggcgc tcgaccgcga cctcgatgcc
     1801 ggccgcgcgc tccactggct ggccgcccag cgcaacgcca gcggcggctt cggctcgacc
     1861 caggacaccg tcgtcgccct ccaggcgatg acgaccgccg ccggcagcaa ccgcgacgac
     1921 atcgatgcga cgatcttcat cagcagcggc gacttcaccc acgaagccca catcgacgcc
     1981 gagaacgccg acatcctcca gctgatcgaa gtcccggccg gcggcacact gacggtcgag
     2041 acgaccggaa ccggccaggt gatgggccag gccgtccgcc gctacaacca gcccgagacc
     2101 ggcgcgatcg agcgcccgat cttcgaccta agcgtcgact acagcgccga cgagatcgag
     2161 gtcgatgaca tcctcgacat tacggcgacg atcacctaca acccgccgga gatgctcggc
     2221 g
//
LOCUS       DASUGJ010000573         9980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1481593, whole genome shotgun sequence.
ACCESSION   DASUGJ010000573 DASUGJ010000000
VERSION     DASUGJ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9980
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481593"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /locus_tag="VFV93_13000"
     CDS             complement(<1..1755)
                     /locus_tag="VFV93_13000"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEX5166312.1"
                     /translation="MSVISKRHPTAMNPSRHPSRYIDSPLRRIAAGLLLAAAVFSVVI
                     ILGAGALLAYGRVAHANTIFTGTSSGGIELGGKSVREAEAALDARFSTFYDEPIPLVL
                     DGKKLNPTPAELGVEFDSHATAEKAYAFGREDSIWRESRRWLDALSGGATVQPVVTID
                     RDAFATYISAHSDELVVAPNDAALVMAPDGGIEIDPGSTGRAVDVGATFERFQQRVET
                     MSNDPIEIATIKLDQATTEADLLPVLDEVNTLAGDPLHLKLNGNVWVISPEDLMRLVS
                     VDVDGDSKVTFDEKAFAGYVRMLEPDIFNPGTDASISNNGGQFILQPAVPGQKLDIRA
                     STDAAVAALRDGRDDVDLVTTPVEPNVTDDEVGAASAEAQRLSSRSITLTWDGGSGVV
                     DRDKLARALRFDINANRNPNVTVTFDEEEMRKALALVANDVKVEGKDAEFRWINDTVE
                     VRTPEQMGRELDVAETSKQLKAALEDGTESIAIVTKDLKPAVTSDMAGTAYIRGKLAE
                     SWTEYGSSVANRLFNVELATSRANGAMVAPGGTFSFNGAVGAVTSENGYKTGYGIVGT
                     SNGSISTIPSVGGGICQVA"
     gene            complement(1870..2880)
                     /locus_tag="VFV93_13005"
     CDS             complement(1870..2880)
                     /locus_tag="VFV93_13005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5166313.1"
                     /translation="MTAFPATVVIVNYNGGETILRCVAALAAQIDPGDIIIVDNASTD
                     GSSRMIHRRYPSVQILPLRHNVGFARAINIAAGRIAHDGALVTINPDTVPKQTFVKSL
                     VQPLAADSQLGSTAGTLVFSSAPTVIASAGICLHRNGVALDDRLGEQLHSNDEPLRPV
                     FGASGGAAAFRLSAFWETGGMAEPFFLYLEDVDLAWRLRLRGWGCVNVASAVATHDYS
                     SSSVEGSAFKRRLLARNRIWTLARCLPNALWTRDRAAIIAFDLLAAGHGTVRLDTASF
                     QGRVAGLAGLPFRLRERSSIHANARVDPEELSTWIRPAISPRRLMELRRLTASLAGPK
                     VT"
     gene            complement(2877..4136)
                     /locus_tag="VFV93_13010"
     CDS             complement(2877..4136)
                     /locus_tag="VFV93_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166314.1"
                     /translation="MPITSELVWDAAAIRRAIRLLGSHSSRIDDPQSGARALRLIWDI
                     VQRRGAPTDIFQLPGTMPLVIVGHGPHLIVTHLDDAHPSAQVDDVGLASISGDVVSVP
                     GITRKAGVLAALGTVLGNDAIAEQVTLIIEADRHSGSRAFAYWLDSTERELHSACCEI
                     ADLPVPAPALFLAATGLATLKVNVTDDGGAFERVYGGVRPDLGHRLVATLAAMKSVDG
                     EVLVPEFYDDVVTPEPEGLAALQQVAAPVGAWLTRGTSPHEDRLTSSHLTLGAFLAPS
                     LNVRALELNGAGPYLANSASAVVEARIMPGQNAAAIARAVSDFIRSRMPDASVDTLLL
                     LPSAKQAALDLATLSTIAPVLPVAPGNSPAGLLEAVGIPTIGFSTVWRDPAAQIEQVN
                     LSAITDGTTMIRSLIELLAESTTGAAG"
     gene            4343..4726
                     /locus_tag="VFV93_13015"
     CDS             4343..4726
                     /locus_tag="VFV93_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5166315.1"
                     /translation="MATTVVATDKAPGAIGPYSQAIKVGGLVFTAGQIPLDPATGKLV
                     EGDIKAQTEQVMKNLQAILDASGSSLSRVVKTTCFLTNLDDFGAFNEVYGSYFSDNKP
                     ARSTVQAARLPAGANVEVECVAECS"
     gene            4762..6498
                     /locus_tag="VFV93_13020"
     CDS             4762..6498
                     /locus_tag="VFV93_13020"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5166316.1"
                     /translation="MADPARWIDEVSADEAMAHTREIAQWVRLSGSEDERKAFDYIER
                     TLRGYGLTTTIHEPTCLVSLPVSGGLEVDGQTIEPCITHSFGVSTPASGLSGEIVYAG
                     RGTASDLAAAGVAGKIALTDGLSTPGKAHAATLAGALAVVSISGDHIHEMIVSPVWGS
                     PTPDRLSLLPQVVMLSIDTAAGEKLKARLAERPARARVFATVDTSWRPIPQLVADLDV
                     DGRDYAMFSGHVDSWHYGAMDNGSANATMIETARILAGHRDELIRGVRFAFWSGHSHA
                     RYAGSTWFADRYWFDLHDHCIAHVNVDSTGAVGATVLSGANTMAEAYPLAREVIGKQS
                     DQQLEYRRFGRAGDQSFWGVGLSSFFMSLSEQGAENEMTADQAFLFGGAKAGGGLGWF
                     WHTTEDTIDKIDPDHLVRDIRVYVETVGRLVTEPVVPLDPRGPIDEMLATLDDIEPLW
                     ATQPGQAPDDTCDFGPLRSELQQARHAAEAVMSRVAGGLNGDAAATVSDAIVAACKAL
                     VPVNYTTAGQFDHDLALDVPALAGLRPPKPLVAMSDDELWGATHALRRELNRVRDGVR
                     RATTSLEAGADA"
     gene            6568..6641
                     /locus_tag="VFV93_13025"
     tRNA            6568..6641
                     /locus_tag="VFV93_13025"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6602..6604,aa:Val,seq:cac)
     gene            6722..7192
                     /gene="greA"
                     /locus_tag="VFV93_13030"
     CDS             6722..7192
                     /gene="greA"
                     /locus_tag="VFV93_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5166317.1"
                     /translation="MAIQGRIQLTPDGKLELERERDLLREVKRPEQLARIQELSSEGD
                     VSDNSEYEDVKEELVIIESRIRDIDNILQDAEIVEAHDSGGVVAFGSTVTIVDDEQFE
                     EVWTIVGPQEANPRLGKISNVSPVGAALLGKRVGEQVTVETPGGETVFTIKDVA"
     gene            7294..8805
                     /gene="lysS"
                     /locus_tag="VFV93_13035"
     CDS             7294..8805
                     /gene="lysS"
                     /locus_tag="VFV93_13035"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX5166318.1"
                     /translation="MELNEQQRVRLEKVRSMRDEGIEPYPPRTERTHTSAEAVSRYES
                     LEATLTDGRDTEEVSVVGRVVGFRDMGKSTFVHIEDGHGRIQLYLRANVIGPEAYEAA
                     THRIDLGDFVQARGRLFRTRTGEVTLEVAEWVMLSKAITPPPEKWHGLTDVEQRYRQR
                     YADLMSNPEVRQIFVTRAKIISAMRRFLDAEGFLEVETPTLQPIYGGAAARPFTTHHN
                     ALDQTLYLRIADELYLKRLIVGGYERVYEICKDFRNEGVDTRHNPEFTQLEFYMAYAD
                     YNDVMDLAERMLSTVTHEVLGTTTITWAGNEIDLSPPWRRLSMAEAVFEATGIDYDKV
                     RDQAELYRLAKEAGADVEPTTVWPRIVDELMKTFVRPKLIQPTFLIDYPVELSPLAKR
                     SPEHAGTVERFQVMIGGVELGNAYSELNDPMDQLERFLEQAKDRSAGDEEAMPIDEDY
                     VQAMMYGMPPTGGYGMGIDRFTMLLTDQQSIREVILFPAMRTRQDGVTDSSES"
     gene            complement(8860..9486)
                     /locus_tag="VFV93_13040"
     CDS             complement(8860..9486)
                     /locus_tag="VFV93_13040"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5166319.1"
                     /translation="MLELPLFPTLESARLAVEECRACPRCTTRDQVVFGLGNPNASLM
                     LVGEAPSPTDNSTGKPFTGPAGRLLDTLLEEAGIHRRHLWITNLVRCFDGALKNGRME
                     NRPVKASEANACRTWLDLEIRYVNPAVILAIGAPAARRIIGSQFRLSEQHGQLIQIDN
                     GRLAVATIQPAYVMRLATINPDAQAAARAQIVDDIRVAARAAGLVGDG"
BASE COUNT         1843 a         2984 c         3404 g         1749 t
ORIGIN      
        1 cgcgacctgg cagataccgc caccaaccga tgggatcgtc gagatcgacc cgttgctggt
       61 accgacgatg ccatagccgg tcttgtagcc gttctcggag gtgacggcgc cgacggcgcc
      121 gttgaacgag aacgtgccgc caggggcgac catcgcgccg ttcgcgcggc tggtggcgag
      181 ctcaacgttg aagaggcggt tggcaactga actgccgtac tcggtccacg attcggccag
      241 cttgccgcgg atgtaggccg ttccggccat gtcggaggtg acggccggct tgaggtcttt
      301 cgtgacgatc gcgatcgact cggttccgtc ttcgagcgct gccttgagtt gcttcgacgt
      361 ctcggcgaca tcgagttcgc ggcccatctg ctccggcgtc cgaacctcga ccgtgtcgtt
      421 gatccagcgg aactcggcgt ccttgccttc gaccttcacg tcgttggcta ccagtgcgag
      481 cgccttgcgc atctcctctt catcgaaggt gacggtgaca ttcggattgc ggttggcgtt
      541 gatatcgaag cgcaatgccc tggcgagctt gtcgcggtcg acgacaccac tgccgccgtc
      601 ccaggtcaac gtgatcgagc gggaactgag tcgctgggct tcagcactgg cggcgccgac
      661 ttcatcgtcg gtgacgttcg gctcgaccgg tgtcgtcacc aggtccacat cgtcgcggcc
      721 gtcgcgcagg gccgcgacgg cggcgtcggt cgaggcgcgg atatcgagct tctggcccgg
      781 gacggccggc tggaggatga actggccgcc gttgttgctg atcgacgcgt cggtgccggg
      841 gttaaagatg tcgggttcca gcatgcggac atagccggcg aacgccttct catcgaaggt
      901 caccttgctg tcgccgtcga catcgacgct gacgagccgc atgagatctt cgggcgagat
      961 gacccagacg ttgccattaa gcttcaggtg caacggatcg ccggccagcg tgttgacttc
     1021 atcgagaacc ggtagcagat ctgcctcggt tgtcgcctga tcgagcttga tcgtggcgat
     1081 ctcgatcggg tcattggaca tcgtctcaac gcgctgctgg aaacgttcga aggtggctcc
     1141 aacgtcgacg gcgcgaccgg ttgagccggg atcgatctcg ataccgccgt ccggcgccat
     1201 gacgagggcg gcgtcgttcg gtgcaacaac gagttcatcc gagtgagcgg agatgtaggt
     1261 ggcgaacgcg tcgcgatcga tcgtcacaac cggctgaacc gtcgcgccgc cggacagtgc
     1321 gtccagccaa cggcgtgatt cacgccagat cgagtcctcg cggccgaagg cgtacgcctt
     1381 ctcggcagtg gcgtgggaat cgaactcgac gccgagttcc gccggagtcg gattgagctt
     1441 cttgccgtcc aggaccagcg gaatcggctc gtcgtagaac gtgctgaagc gagcgtcgag
     1501 cgccgcctcg gcctctctca ctgatttccc gcccagctcg atgccgccgg aactggtccc
     1561 ggtgaagatc gtgttggcgt gggcgacccg gccgtaagcc agcagcgcgc cggcgccgag
     1621 gatgatgaca actgagaaga cggccgccgc cagcagtagc ccagccgcga tacgccggag
     1681 cgggctatca atgtagcggg aaggatggcg agacggattc attgcggtcg ggtggcgctt
     1741 agagattact gacattggaa accagttggg ccttcctgaa cgtcgttctc gacgtaatgc
     1801 tggagatagt atgccagcca ggatcctgtc attgtgtacg tacagtctac gctgccgggt
     1861 ttgtggctgc taggtgacct ttggcccagc cagtgaggcg gtcaaccggc ggagctccat
     1921 caggcggcgt ggcgagatcg ccggacgtat ccaggttgac agttcctcag gatcaaccct
     1981 cgcattcgca tggatgctgc tccgttcgcg cagccgaaac ggcagaccgg cgaggccagc
     2041 aactcgtccc tgaaaggacg cggtgtccaa ccgaacggtt ccgtggccgg cagcgagcag
     2101 gtcgaaggca atgatggcgg cgcgatcacg ggtccagagt gcattcggca agcagcgtgc
     2161 cagagtccag atccgattgc gcgccagcag gcggcgcttg aacgccgatc cctcaacaga
     2221 cgacgatgag tagtcatgcg tggcaacggc tgacgcgaca ttgacgcatc cccagcctct
     2281 caagcgcaga cgccaggcga gatcgacgtc ctcgaggtag aggaagaacg gctcagccat
     2341 cccgccggtt tcccagaagg cgctcagccg gaaggcggcc gcgccgccgc tggcgccgaa
     2401 gactggtcgc agaggctcgt cgttggagtg cagttgctcg ccgaggcggt cgtcgagcgc
     2461 gacgccgttg cggtgcaggc agatgccggc cgaggcgatg accgtcggcg ccgacgagaa
     2521 gacgagcgtt ccggcagtcg agccgagttg agagtcagct gccagcggct gcaccaggga
     2581 tttcacgaac gtttgtttag gtacggtgtc cggattgatg gtcaccagcg cgccatcatg
     2641 tgcgattcgg ccggcggcaa tgttgatcgc gcgggcaaag ccgacgttgt gacgcaacgg
     2701 caggatctga accgacggat agcgtcgatg aatcatgcgc gacgaaccgt cggttgacgc
     2761 gttatcgacg atgatgatgt cgccgggatc gatctgggcg gcgagggcag ccacgcagcg
     2821 cagtatcgtt tcgccgccgt tgtagttgac gatgacgacc gtcgccggaa acgctgtcac
     2881 cccgccgcgc ccgttgtcga ttcggcgaga agctcgatca gagaccgaat catcgtcgtt
     2941 ccgtccgtaa tggccgaaag attgacctgc tcgatctggg cggcaggatc gcgccagacg
     3001 gtcgaaaagc cgatcgttgg aatgccgacg gcttccagca agccggccgg ggagttgccg
     3061 ggagcgaccg gcaggaccgg tgcgatggtt gagagcgtgg cgagatcgag cgccgcctgc
     3121 ttcgcagaag gtagtagcag tagcgtgtcg acagacgcgt ccggcatccg cgaacggatg
     3181 aaatcggaga cagcccgcgc gatcgcggcg gcgttctggc ccggcatgat gcgcgcctcg
     3241 acgacggcgg atgcactgtt cgccagatac ggtcccgcgc cattgagctc aagggcacgg
     3301 acgttgagcg acggcgccag aaaggcgccg agtgtcagat ggctcgacgt caggcggtcc
     3361 tcgtgtggag aggtgccacg tgtcaaccag gcgccgaccg gagctgctac ctgctggagt
     3421 gctgccaggc cctctggctc cggtgtcaca acgtcgtcgt aaaactctgg gacgagcacc
     3481 tcgccgtcaa ccgacttcat ggcggccagc gtggcaacaa gccggtgacc aagatcgggc
     3541 cggacgccgc catagacgcg ctcgaacgcc ccgccgtcgt cggtgacgtt gaccttcagg
     3601 gtcgcgaggc cagtcgcggc cagaaagagc gccggcgcgg gaaccggaag atcggctatc
     3661 tcgcagcagg ctgagtgcag ttcgcgctcg gtgctgtcga gccagtaggc gaacgcccgc
     3721 gagccgctgt gacgatccgc ctcgatgatc agcgtgacct gttcggcgat cgcgtcgttg
     3781 cccagcacgg tgccgagcgc cgcaaggacg cccgccttgc gcgtgatgcc gggtacgcta
     3841 acgacatcgc cggagatcga ggccagaccg acatcatcga cctgcgccga agggtgggca
     3901 tcgtcgagat gcgtcacgat caggtgggga ccgtggccga cgatgacgag tggcatcgtg
     3961 ccgggcagtt gaaagatgtc ggtcggcgcg cctcggcgtt ggacgatgtc ccagatcagc
     4021 ctcaacgcac gggcgccgga ctgtggatcg tcaatcctgg aggagtgtga accgagcagg
     4081 cggattgccc ggcggattgc ggctgcgtcc cagacgagtt cagatgtaat tggcatctgt
     4141 tcaccagatt tgcgagttcg cgatcgttca gttcgttcgg caggctaccc tcgtcgggag
     4201 agtgtacccg acctcggctg gcgcatggtt gcgctttgtt ggacgttgac cgcgcctctg
     4261 actggtggta gaaggcagac ggcgctcgcg gacaggatgc gcgtacgtcg tgagtcgaaa
     4321 cgtaacaggg aagggttcaa gaatggcgac gacggtagtt gcgacggaca aggctccggg
     4381 cgcgatagga ccatactcgc aggcgatcaa ggtcggcggg ctggtcttca cagcaggtca
     4441 gattccgctc gatccagcga ccggcaagct ggttgaggga gatatcaagg cgcaaaccga
     4501 gcaggtcatg aagaacctgc aggcgatcct ggatgcgtcg ggatcgtcgc tgtcgcgcgt
     4561 cgtcaagaca acctgcttcc tcacaaatct ggacgacttc ggggcgttca acgaggtcta
     4621 cggcagctat ttctcggaca acaagccggc ccgctcgacc gtccaggcgg cgcgcctgcc
     4681 agccggcgcg aacgtcgagg tcgagtgcgt ggctgagtgc agctagtcag agtcgagttg
     4741 tagagaacgg cgaggctgac catggcagac ccggcacggt ggatcgacga ggtttcggca
     4801 gatgaggcga tggcgcacac acgcgagatc gcgcagtggg tgcggctctc cggctccgag
     4861 gacgagcgca aggcgtttga ctacatcgaa cggaccttgc ggggctacgg gctgacgacg
     4921 acgattcacg agccgacctg cctggtcagc ctgccggtct ccggcggtct ggaggtcgac
     4981 ggccaaacga tcgagccgtg catcacgcac tcgttcggtg tttcaacacc tgcgagcggc
     5041 ttgtcgggcg agatcgttta cgccggtcgc gggacagcca gtgacctggc ggcggccggg
     5101 gtggccggca agatcgcgct gaccgatggg ctgagcactc ccgggaaagc acacgcggcg
     5161 acgctggccg gggcgcttgc cgtcgtcagc atctccggtg accacatcca cgagatgatc
     5221 gtctcgccgg tctggggctc accgacgccc gaccggctga gcctgttgcc acaggtcgtg
     5281 atgctctcga tcgacacggc ggccggcgag aagctgaaag cgcggctcgc ggagcgcccg
     5341 gcgcgagcac gcgtgttcgc gaccgtcgac accagctggc ggccgatccc gcagcttgtc
     5401 gccgatctgg acgttgacgg tcgcgactac gcgatgttca gcgggcatgt cgattcgtgg
     5461 cactatgggg cgatggacaa tggctcggcc aacgcgacga tgatcgagac ggcccgcatc
     5521 ctggctggac accgtgacga gctgattcgc ggtgtccgct tcgcgttctg gtcgggccac
     5581 tcacacgccc gctacgccgg ctcaacctgg ttcgccgatc gctactggtt cgatctgcac
     5641 gaccactgca ttgcacacgt caacgtcgat tcgaccggcg cggtcggcgc gacggtgctg
     5701 agcggggcga acacgatggc cgaggcgtac ccgctggcgc gggaagtcat cggcaagcag
     5761 tccgatcagc aactcgaata ccggcgcttc ggccgggccg gtgatcagtc cttctggggc
     5821 gtcgggctgt cgtcgttctt catgtcgctc tccgaacagg gcgccgagaa cgagatgacg
     5881 gccgatcagg cgttcctgtt cggcggcgcg aaggcgggcg gcgggctggg ctggttctgg
     5941 cacacgacgg aggacacgat cgacaagatc gatcctgatc atctggtgcg cgacatccgc
     6001 gtctatgtcg agacggtcgg gcgactcgtc accgagccgg tcgtcccact cgatccgcgt
     6061 ggcccgatcg acgagatgct ggcgacactg gacgacatcg agccgctctg ggcgacccag
     6121 ccgggacagg cgcctgatga cacatgcgac ttcggccctt tacgttccga gcttcagcaa
     6181 gcgagacatg ctgctgaggc ggtgatgtcg cgcgttgccg gcgggctcaa tggcgatgcg
     6241 gctgcaacgg tatccgacgc gatcgtggca gcctgcaagg cgctggtgcc ggtgaattac
     6301 acgacagccg gccagttcga ccacgatctt gcgctggatg tgccggcatt ggcagggcta
     6361 cgaccgccca agccactggt tgcgatgagc gacgacgaac tgtggggagc gacccatgca
     6421 ttgcgccgcg aactgaatcg tgttcgggac ggcgttcgcc gcgcaacgac atccctcgag
     6481 gctggtgcag acgcgtgacg atgtgtcacg agagcggtcg gattcgggcc tgagagtcac
     6541 tcgttcgtgg tatactcgaa ccgaccaggg cgcgtagctc agctggttag agcgcacggt
     6601 tcacatccgt gaggtcactg gttcaagtcc agtcgtgccc accgaggcgg ccacattttg
     6661 tggccgtttg tcgcatgcgt cgtattcctc gcctggcgag gatgcggaga gtgagacgaa
     6721 gatggctatc cagggtcgaa ttcagttgac accggacgga aagctcgagc tggagcgcga
     6781 gcgggacctg ctgcgggagg tcaagcgacc ggagcagctt gctcgtatcc aggaactgag
     6841 ttccgaaggt gacgtgtccg acaacagcga gtatgaggat gtcaaggaag agcttgtcat
     6901 tatcgaatcg cgcatccgcg atattgacaa tatccttcag gacgccgaga tcgtcgaggc
     6961 gcatgactcc ggcggcgtcg tcgcgttcgg ctcgacagtg acgatcgtgg atgacgaaca
     7021 gttcgaagaa gtctggacga tcgtcggacc acaggaagca aatccccggt tgggcaagat
     7081 ctccaacgtc tcgccggtcg gcgccgcgtt gctcggtaaa cgggtcggtg agcaggtcac
     7141 ggttgagacg ccgggcggcg aaacagtctt cacgatcaag gacgtcgcat agcgtccgac
     7201 acacgggtca tgcagacttg taccgcggga gccggcaacg gccccgcggt ttcctttttg
     7261 cgagcgaatc aggcgacgga cggagacgta ccaatggaac tcaacgagca acagcgtgtc
     7321 aggctggaaa aggtgcgctc catgcgtgac gaggggattg agccttatcc acctcgtacc
     7381 gaacgcacgc acacgtcagc cgaagcggta tcgcgctacg agagccttga agcgacgctg
     7441 acggacggcc gcgataccga ggaagtgtcc gtcgtcggac gggtcgttgg gtttcgtgac
     7501 atgggcaagt cgacgttcgt ccacatcgag gatggacatg gccggattca gctgtatctg
     7561 cgagcgaacg tgatcggccc tgaggcgtac gaggctgcca cgcatcggat cgacctgggc
     7621 gactttgtcc aggcccgcgg tcgactgttc cggacacgca ccggcgaggt gacacttgag
     7681 gtcgcagaat gggtgatgct gtcgaaggcg atcacgccgc cgccggagaa gtggcacggc
     7741 ctgacagatg tcgagcagcg ctatcgccag cgctacgccg acctgatgtc caacccggag
     7801 gtgcgccaga tcttcgtgac gcgtgcgaag atcatcagcg cgatgcggcg cttcctcgac
     7861 gccgaaggat tccttgaagt cgagacgccg acgctgcagc cgatctacgg tggcgcggcg
     7921 gcgcggccgt tcacgaccca tcacaacgcg ctcgaccaga cactgtacct gcggatcgcc
     7981 gacgaactct atctgaagcg gctgatcgtt ggcggctacg agcgggtcta cgagatctgc
     8041 aaggacttcc gcaatgaggg cgtcgacacg cggcacaacc cggagttcac ccagcttgag
     8101 ttctacatgg cctatgctga ctacaacgat gtcatggacc tggccgagcg gatgctctcg
     8161 accgtgacac acgaggtgct ggggacgacg acgatcacct gggcaggcaa cgagatcgac
     8221 ctcagcccgc cgtggcggcg gctatcgatg gccgaggcgg tgttcgaggc gaccggcatc
     8281 gactacgaca aggttcgtga tcaggcggaa ctctaccggc tggcaaagga ggccggcgcg
     8341 gatgtcgagc caacgacggt ctggccgagg atcgtcgacg agttgatgaa gacgttcgtg
     8401 cgcccgaagc tgatccagcc gacgttcctg atcgactatc cggtcgagct ctcgccgctg
     8461 gccaagcgaa gccccgagca tgcgggcacg gtcgagcgct tccaggtcat gatcggtggc
     8521 gtcgagctgg gcaatgccta ctcggaactg aacgacccga tggaccagct ggagcgcttc
     8581 ctggagcagg ccaaggaccg ttcggccggc gacgaggaag cgatgccgat cgacgaggac
     8641 tacgtccagg ccatgatgta cgggatgccg ccgacgggag gatacgggat gggcatcgat
     8701 cgcttcacga tgctgctgac cgaccagcaa tcgatccgcg aggtgatcct ctttcctgcg
     8761 atgcgaacgc ggcaggatgg cgtgacggat tcatcggagt cgtgatcggt gacgcgtgac
     8821 gcgaccggcg agtccctcaa tcgcggaact tgtcacgcgt cacccatccc cgacgaggcc
     8881 ggctgcgcgg gcggcgacgc ggatgtcgtc gacgatctgg gcgcgggctg ctgcctgggc
     8941 gtcggggttg atcgtggcga gccgcatgac gtaggcgggc tggatcgttg cgaccgccag
     9001 ccggccgttg tcgatctgga tcaactggcc gtgctgctcg ctcaatcgaa actgcgaccc
     9061 gatgatacgt cgcgctgccg gggcgccgat ggcgaggatg acggctgggt tgacatagcg
     9121 gatctcgagg tccagccagg tccggcaggc gttcgcctcg ctcgccttga cggggcgatt
     9181 ctccatccgg ccgttcttga gcgcgccgtc gaagcagcga acgaggttgg tgatccagag
     9241 atggcggcgg tggataccgg cctcctccag gagcgtgtcg agcagacgcc cggccggccc
     9301 ggtgaacggt ttgccggtgc tattgtcggt cgggctgggt gcctcgccga ccagcatcag
     9361 cgacgcgttc ggattgccca gtccgaagac gacctggtca cgagtggtgc aacgcgggca
     9421 ggcgcggcac tcttcgactg ccagcctggc ggattcgagg gttgggaaaa gcgggagttc
     9481 aagcattgtg accccttatc ctcggtcatg ccggaggagg cgcaatctga gtctgtgaaa
     9541 tcctcaacga tccgataacc ctatgctata ctcgcgaacg aactcgccga ccgcgccaac
     9601 cgggggtggc gctgggcgga gtgagcgttc tggacccgtt tcagtcggct actcggcgtc
     9661 ctgcttcgca tcctgccaaa accggccttt ccgaatcggg tactatatgg cggcgtggtc
     9721 ctgaccgcgg acatcatcct tccatgcgcc ggccaaccgc gctcaatctg gacaggcgtg
     9781 agggttgtcg ccggcagggg gcgacgagag acgaggggga cgatgcgacg agagcagacg
     9841 atcgccgatc gagtcacgag cagcgcgctg tggcgatcga tcgttcgaca tggctaccct
     9901 gatacgcagc gcaaccaggc gctgattgtc gcatccaacg tcttcctcca cctgcaccca
     9961 gtcaagatca agcgctacgc
//
LOCUS       DASUGJ010000574         7058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_92653, whole genome shotgun sequence.
ACCESSION   DASUGJ010000574 DASUGJ010000000
VERSION     DASUGJ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92653"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFV93_13045"
     CDS             complement(<1..275)
                     /locus_tag="VFV93_13045"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX5166320.1"
                     /translation="MNTLLLILVMAACVYTIRLGGFVLARATIPAGWELTLGYVPIAT
                     LTALVVASLAGRDDHLPQGIVAAALAVAISRLTRRTWACITGGMVVY"
     gene            complement(272..1009)
                     /locus_tag="VFV93_13050"
     CDS             complement(272..1009)
                     /locus_tag="VFV93_13050"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX5166321.1"
                     /translation="MAHQSATLEPTTQHASQQTTTNRALFVRGFVGLMPLWAGAIPVG
                     IAYGLAARGAGLSIIEAQLMSLTVFSAAAQVSAVSLLDDGAPAIVILATALLLNVQLL
                     LLGLAAGRVAQPSWRGRLLGAGLLTEGAYGVAVGTGKLTLPSLVGAGASMYLAWNVGT
                     ALGITVGDAIPNPRGIGIDLVAPLTFLAVLVPLVRTRPAIVTVLAAGATALLLAQIAP
                     TGVAVLGAGLGGSMAGGWAARRPDRTR"
     gene            1113..1973
                     /locus_tag="VFV93_13055"
     CDS             1113..1973
                     /locus_tag="VFV93_13055"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5166322.1"
                     /translation="MEAGEREPGTAGGRRLRAIRRAAGRTQLWVEAESELGTGYLQRV
                     ESGRVVQPERGTLERILNALDARYSERREVLELFGYAVSTPPPDDADITWARATCHDE
                     LHAVPFPAIVLDCTHRLLAWNRLVPRLFGILPDDPTFGRLAGRSYITAWFDPSSPIPP
                     HVAEPDALLPAILRAFQYEMRQFGHEPWYAGIMAELRALPAFQHYWRVTEQTPPPVGA
                     ARALVPLRLSVPGSGTLEFRLASEPFVRDARFRMVFYVPGDLVTMQWCAEWSGFGNRD
                     SGQGSIVPSS"
     gene            complement(1995..2771)
                     /locus_tag="VFV93_13060"
     CDS             complement(1995..2771)
                     /locus_tag="VFV93_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5166323.1"
                     /translation="MRLTILGGAAAGPGPHQGCSGYLIEAGGARIVLDLGPGTLTELR
                     HQTELPALDAIVVSHMHVDHILDLFSLWWGWIHTPQPLPRLLSLWLPPGGHDRLMATV
                     RTLAAEPDEIERLATRVFHVREYDPSAPLQIGAATLRFAPTRHYIPCWAIRVDVAGES
                     SLIYTADNGDAARLVPFAAGAGVLIAEASLPDVPPVADAGRGVSAATEVAALARDAGV
                     QTLVLTHIWPEHDAEAARTTAASVFNGRIEIARPGLTLCS"
     gene            complement(2917..3939)
                     /locus_tag="VFV93_13065"
     CDS             complement(2917..3939)
                     /locus_tag="VFV93_13065"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX5166324.1"
                     /translation="MQRTHRTSRIFWLLTSVLMLLGAVPATTAAKGGPELWIMGQDTS
                     RIFILKGSDLQETVQLPAGTGPHMTRFSPDGRFAYVSGVVSGDLIVLDAQTHEIIQTL
                     DLGTSGVHEAAPSPDGSELFVAQQTTRELIRVAVDPQTHMVTETGRLALPASPVCTIL
                     MPDSNKAYVTLSGQDLAIVDTVSMQITGMIDINGQARCNYDWATNGKLLYLTSENGTD
                     SFVYVVDPTTDTPSLLYTFSGVRDIHSLVVNPNEKQISIIGRASDRYYTLTLSDLSVT
                     SFELGTPGVSDQPDAMVIVGNTAYVNLKASGRLATIQLNKGTISYLDLATPSANAALN
                     VIRRHD"
     gene            complement(4218..6641)
                     /locus_tag="VFV93_13070"
     CDS             complement(4218..6641)
                     /locus_tag="VFV93_13070"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5166325.1"
                     /translation="MTGLPHPRTALIGRHNEIETASALLRSGETPLLTISGPGGVGKT
                     RLALEVARRLSGEFPDGVVFVPLATVRAPAQLGPTIARALGVQATGLGTSHEGLIAHI
                     GERRLLLVLDNLEHLLDAVPLLAELLLGCPNLTILATSRAILRVSGERVMSLQPLPVP
                     STGGFGGPAELRRNPAVILFNERATAVRPDFRMTDDLLPTVAAICDRLDGLPLAIELA
                     AARIKVLSPTALLERMTQLLPLLTSGARDAPERQRTLRSTIDWSYQLLDERLQATFRR
                     LAIFVGGWSVEGAAVVSDEQPLSEVDALDALEALIEHNLIVEVPGPFRQPRFTMRETI
                     REYGLELLAATGELDATASRHTRYYVDYAERNDPKLYAYPIDPPIIDRWVAEHDNIRS
                     VLRRTIDNGDAATALQLCGAMRRFWNSRSDYREARDWLAAALALPGDVPDPLRARALD
                     AFGAMELNLGEYEIGLASIQRALMAYRAQENRAREARVMSDPPMVALLRGDLDMAERG
                     FQDTLDFALTFPDAAWMAVGMRRALGEVAFARGDLSSAQAYVEASLAEARAIGHEWAV
                     TLGLTLLGRIATERGEYDVAEALFRESIPIALRSRQEGFASANYVRLGQLSLLQCKNR
                     DAATWFQTCLNSSPNTLDETVECVEGVAAVAGVLGHGQTAALLYGATSEWRSRTGVIV
                     PRSRQHEHERHLENARASIEPDRWQAAFDAGRRLPMDAAIEHARQLEPPADASRPSQP
                     DGLRPLLTSRERDVLRLLAAGRSNREIGDTLYLSVRTVERHIANLYGKIDAHNRAEAT
                     AWAIAHPTD"
     gene            complement(6663..>7058)
                     /locus_tag="VFV93_13075"
     CDS             complement(6663..>7058)
                     /locus_tag="VFV93_13075"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5166326.1"
                     /translation="GKVCGIESSEVLLAEAQERNAEAIGGGYVELKTGMTSRLPWPEA
                     TFTKAYAVNSPRDWPQPVVNLAEIRRVMQPEGRLAIVIQPPWRTARDGVRDLAELWAH
                     RVDEAGFRDVRFGILRAGRSSAAAITGTV"
BASE COUNT         1099 a         2310 c         2459 g         1190 t
ORIGIN      
        1 cagtagacga ccatcccgcc ggtgatacag gcccaggtcc ggcgcgtcag tcgcgagatg
       61 gcgaccgcca gcgccgccgc gacgatgccc tgcgggaggt ggtcgtcgcg cccggccagg
      121 ctggcgacga ccaacgccgt cagcgtcgcg atcgggacgt agccgagcgt cagctcccag
      181 ccggcgggga tcgtcgcccg ggccagcacg aacccgccaa gccggatcgt gtagacgcag
      241 gcggccatga ccagaatcag cagcagggtg ttcatcgcgt tcggtccggc cgtcgcgccg
      301 cccagccgcc ggccatgctg ccgccgagcc cggccccgag gacggccacg ccggttgggg
      361 cgatctgcgc cagcagcaac gccgtcgcgc cggccgccag gaccgtcaca atcgccggtc
      421 gggttcgcac gagtgggacg aggacggcca gaaacgtcag cggtgcgacc aggtcgatcc
      481 cgattccgcg cgggttggga atcgcatcgc caaccgtgat cccgagcgcc gtcccgacgt
      541 tccaggcgag gtacatgctg gccccagccc cgaccaaact gggcagcgtc agcttgccgg
      601 tcccgacggc aacgccatag gcaccctcgg tcagcaggcc agcgcccagc agccgcccgc
      661 gccagctcgg ctgcgcaacc cggcccgcgg ccagtccgag cagcaggagc tggacgttga
      721 gcagcagcgc cgtcgccagg atgacgatgg ccggcgcgcc gtcatccagc agcgagacgg
      781 cgctgacctg ggcggcggcc gagaagacgg tcaggctcat cagctgcgct tcgatgatac
      841 tcagtcccgc gccccgcgct gcaagcccgt aggcgatccc aaccggaatc gccccggccc
      901 agagcggcat cagcccgacg aacccgcgca cgaagagcgc ccggttggtc gtcgtctgct
      961 ggctggcgtg ctgcgtggtt ggttccagtg tcgcggactg gtgtgccatc gtgacctgtc
     1021 ctggatgcga acgtcgtgcc ttccgctatc ctagaaagtg tcgcggggcg agtcatcgtc
     1081 accggagtcg aattctggcg gtaaggggcg cgatggaggc cggggagcgc gaaccgggca
     1141 cagcgggcgg caggcggctg cgggcgatcc gccgggcggc cggtcggacg cagctctggg
     1201 tcgaggccga gtcggagctg ggcaccggct acctgcaacg ggttgaatcc ggtcgggtcg
     1261 tccagccgga acggggaacg ctggagcgaa ttctcaacgc gcttgatgca cgctacagcg
     1321 agcgccgcga ggtgctggag ctgttcggct acgccgtctc caccccgccg ccggacgacg
     1381 ccgacatcac gtgggcgcgg gcaacctgtc acgacgaact gcacgccgtc ccatttccgg
     1441 ccatcgtcct cgactgcacc caccggctgc tggcctggaa ccggctcgtc ccgcgcctgt
     1501 tcgggatcct gccggacgac ccgacgttcg ggaggctggc aggcaggtcc tacatcacgg
     1561 cctggttcga cccttcctca ccaatcccgc cgcatgtcgc cgagccggac gcgctgctgc
     1621 cggcgatcct ccgcgccttt cagtacgaga tgcgccagtt cggccacgag ccgtggtacg
     1681 ccgggatcat ggccgagttg cgggcgctgc cggcctttca gcactactgg cgcgtgaccg
     1741 agcagacccc gccgccggtc ggcgccgccc gtgcgctcgt cccgctacgc ctaagcgtcc
     1801 ccggcagcgg cacgctcgag ttccggctgg cctcggaacc attcgtccgc gacgcccgct
     1861 tccggatggt cttctacgtc cccggggatc ttgtcacgat gcagtggtgc gcggagtggt
     1921 cgggattcgg gaatcgggat tcggggcagg ggagcatcgt accatcttcg tagggaccgg
     1981 tctgagacca gtccctacga gcacagcgtc agccccggcc tcgcgatctc gattcgaccg
     2041 ttaaagacac ttgctgccgt ggttcgtgcc gcttcggcat cgtgctcggg ccagatgtgc
     2101 gtcaggacga gcgtctggac accggcgtcg cgcgcgaggg cggcgacctc agttgcggcg
     2161 gagacaccac gacctgcatc ggcaacgggt ggcacatccg gcagcgacgc ttcggcgatc
     2221 aggacaccgg cgccggccgc gaacgggacg aggcgggcgg catcgccatt gtcggcggtg
     2281 tagatgaggc tggattcccc ggcgacatca acgcggatgg cccagcaggg aatgtagtgg
     2341 cgcgtcggcg cgaagcggag cgtcgccgcg ccgatctgaa gcggcgcgga cgggtcgtac
     2401 tcgcggacat ggaacacccg cgtcgccagc cgctcgatct cgtccggttc ggcggccagc
     2461 gttcggacgg ttgccatcag ccggtcgtgg ccaccgggcg ggagccagag ggacagcagc
     2521 ctggggaggg gctggggagt gtggatccag ccccaccaga gactgaacag gtcgaggatg
     2581 tggtcgacgt gcatgtgcga gacgacgatc gcatccagcg caggcagttc cgtctggtgg
     2641 cgcagctcgg ttagcgtgcc tggaccgaga tcgaggacga tgcgcgcccc gccggcctcg
     2701 atcagatagc cggagcaacc ctggtgtgga cccggcccgg ccgccgcgcc gccgaggatc
     2761 gtgagtcgca tgtgtgtcgc ctcattcacc agcttcccct agtgtcgtca ttcgctctgc
     2821 ctgtccgcca cctcaggata ccgcgtggca cggtcaggcc agcgccccgg atcgagtcga
     2881 cccagggcgc tggctctgtt gccggacgac cgggttctag tcgtgccggc ggatgacgtt
     2941 gagggctgcg ttggcggatg gggtggcgag gtcgaggtac gagatcgtgc ccttgttgag
     3001 ctggatagtc gccagcctgc cgcttgcctt gaggttgacg taagcggtgt tgccgacgat
     3061 caccatcgcg tccggctggt cggaaacacc tggggtgccc agctcgaacg aggtgaccga
     3121 caggtcgctg agtgtgagcg tgtagtaccg gtcactggcg cgcccgatga tgctgatctg
     3181 cttctcattc gggttgacga ccagcgagtg gatgtcgcgg acgccgctga acgtgtacag
     3241 cagactcggc gtgtcggtcg ttggatcgac gacatagacg aagctgtcgg ttccgttctc
     3301 ggaggtcagg tagagcagct tcccgttggt cgcccagtcg tagttgcagc gcgcctggcc
     3361 gttgatgtcg atcatgccgg tgatctgcat cgagacggtg tcgacgatcg ccagatcctg
     3421 acccgagaga gtgacgtagg ccttgttgct gtcaggcata aggatcgtgc agaccgggct
     3481 ggccggtagc gcgaggcggc ccgtttccgt caccatgtgt gtctgcggat cgacggcgac
     3541 gcggatcagc tcgcgggtcg tctgctgggc gacgaacagc tccgagccat ccggcgacgg
     3601 cgctgcctca tggacgccgc tcgtgccgag gtcgagcgtc tggatgatct catgcgtctg
     3661 cgcatcgagg acgatcagat cgccgctgac gactccggag acgtaggcga acctgccgtc
     3721 cggcgagaag cgcgtcatgt gcggcccggt gccggccggc agctggaccg tctcctgcag
     3781 gtccgatccc ttgaggatga agatgcggct cgtgtcctgg cccatgatcc atagctcggg
     3841 accgccctta gctgccgttg tcgccggcac ggcgccaagc agcatcagga ccgacgtcag
     3901 cagccagaag attcgagagg tccgatgtgt tcgttgcacg acatgtctcc tgtcattgtg
     3961 gttgtcggga tatcgtccga tgcccggttc gtgctgcgtt gctgggttgc gcgccatgtc
     4021 gaactcctct ctgtaccttg tcgagacgca ggtcgagggt ccgccgggcg gcagccaagc
     4081 ggaaaactcg atcgacgtcc ggcgaatccc tcgacccttc gactgtagtg cggtgggcag
     4141 gcgcatgtcg tccgtgcaac tgcgtagttg ccggccggtc gggggaattg ggtactacgt
     4201 agaggaggtg gcagcgatca gtccgtgggg tgggcgatcg cccaggcggt tgcttcggcg
     4261 cgattgtgcg cgtcgatctt gccgtagagg ttggcgatat ggcgttcgac ggtcctgacg
     4321 ctgaggtaga gcgtgtcgcc gatctcgcga ttgctccggc cggcggccag cagccgcagg
     4381 acgtcgcgct cgcgcgacgt cagcaacggt cggagcccgt ccggctggct ggggcgtgag
     4441 gcatcggccg gaggttcaag ttgccgggca tgttcgatgg cggcatccat cggcagccgc
     4501 cgaccggcgt cgaacgcggc ttgccagcga tctggttcga tgctggcgcg cgcattctcc
     4561 agatggcgct cgtgttcgtg ttgccgcgat cgtgggacga tgacgccggt tcgcgatcgc
     4621 cactcgctcg tcgccccata cagaagagcg gcggtctgcc catgtccaag gactccggca
     4681 actgccgcga cgccttcgac acactcgacg gtttcgtcga gtgtattcgg gctggagttg
     4741 aggcaggttt ggaaccatgt tgccgcgtcg cggtttttac attggagaag actgagttgc
     4801 ccgaggcgaa cgtagtttgc gctggcgaat ccttcctggc gcgaccggag ggcaatcggg
     4861 atgctctcgc gaaagagggc ttctgccacg tcgtactcac cgcgctcggt cgcgatgcga
     4921 ccgagcaagg ttagccccag tgttaccgcc cactcgtggc cgatcgcgcg cgcttcggcg
     4981 aggctggctt ccacatacgc ctgagcactg gagagatcgc cgcgtgcgaa cgcgacctcc
     5041 ccgagtgctc gcctcatccc gaccgccatc caggctgcgt cggggaaggt cagtgcgaaa
     5101 tcgagtgtgt cctgaaatcc ccgttccgcc atatcgagat cgcctcgcag gagtgcaacc
     5161 atgggcggat cgctcatcac gcgcgcctca cgtgcccggt tttcctgcgc acggtaggcc
     5221 atcagcgccc gttgaatcga agccaggccg atctcgtact caccgaggtt gagttccatc
     5281 gcgccgaatg cgtcgagcgc gcgtgcacgg agtgggtccg gcacatctcc tggcagagcg
     5341 agtgctgccg ccagccagtc acgcgcctcg cgatagtctg accgcgagtt ccagaagcgg
     5401 cgcatcgcac cgcaaagctg gagggcggtc gccgcgtcgc cgttgtcgat cgtccgtcgc
     5461 aggactgagc ggatgttgtc gtgctcggcc acccatctgt cgatgatcgg ggggtcgatg
     5521 gggtacgcat agagcttcgg atcattccgc tcggcataat cgacataata ccgcgtgtgg
     5581 cgtgaggcgg tcgcatctag ttcgccggtc gcggcgagca gttccagtcc gtattcccgg
     5641 attgtctcgc gcatcgtgaa gcgcggctgt ctgaacggcc cgggaacctc gacgatcagg
     5701 ttgtgctcga tgagggcctc cagcgcgtcg agggcatcga cctcgctgag cggttgctcg
     5761 tcgctgacga cggcggcgcc ctcgactgac cagccgccaa cgaagatcgc caggcggcgg
     5821 aaggttgcct gcagccgctc gtcgagcagt tggtagctcc agtcgatcgt cgaacgaagc
     5881 gtccgttgcc gttcgggcgc atcgcgtgca ccgctcgtca acaacggcag cagctgggtc
     5941 atcctctcca ggagagccgt cggagaaagc accttgatgc gtgctgctgc cagctcgatc
     6001 gccagcggca ggccatccag ccggtcgcag attgcagcga cggtcggcag caggtcgtcg
     6061 gtcatccgaa agtcggggcg aacagcggtc gcgcgctcgt tgaacaggat gaccgcaggg
     6121 ttccggcgca gctcggccgg ccccccgaag cctcccgtcg acgggaccgg cagcggctgg
     6181 agcgacatga cgcgctcgcc gctgacgcgc agaatggccc ggctcgttgc aaggatcgtc
     6241 aggttcggac agccgagcag cagttcggct agcagcggga cggcgtccag caggtgttcg
     6301 aggttgtcga ggacgagcag caggcgccgc tcgccgatgt gagcaatcaa cccctcatgt
     6361 gatgtcccga gtccggttgc ctgaacgccg agcgcccgcg cgatcgtcgg gccgagctgg
     6421 gcgggcgccc ggacggtggc aagcgggacg aagacgacgc catccgggaa ctcgccgctc
     6481 agtcgccggg cgacttccag cgccagccgc gtcttgccga cgccgcccgg gccgctgatc
     6541 gtcagcagcg gcgtctcgcc ggagcgcagc agggcgctgg ccgtctcgat ctcgttgtgc
     6601 cggccaatca gcgcagtccg tgggtgaggc aggccagtca tctgctgggt gtctcccggc
     6661 tgtcagaccg ttccggtgat ggctgccgcg ctgctgcggc ccgcgcgcag gatgccgaag
     6721 cgaacatcgc ggaagccggc ttcgtcgacg cggtgcgccc agagctcggc caggtcgcga
     6781 acgccgtcgc gggccgtccg ccagggcggc tggatgacga tggccagccg gccctccggc
     6841 tgcatcacgc ggcggatctc ggccaggttc acgaccggct ggggccagtc gcgcgggctg
     6901 ttgacggcgt acgccttcgt gaacgtcgcc tccggccagg gcagtcgcga ggtcatgccg
     6961 gtcttcagtt ccacataccc gccgccgatc gcctcagcgt tccgctcttg tgcttccgcc
     7021 agcagcacct ccgatgactc gatcccgcag accttgcc
//
LOCUS       DASUGJ010000575         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_233522, whole genome shotgun sequence.
ACCESSION   DASUGJ010000575 DASUGJ010000000
VERSION     DASUGJ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233522"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..2640)
                     /gene="lon"
                     /locus_tag="VFV93_13080"
     CDS             complement(169..2640)
                     /gene="lon"
                     /locus_tag="VFV93_13080"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5166327.1"
                     /translation="MSRYDLYAVRYPLLPLKNVVIFPRNVVTLLVGRTRSIQAVEEAM
                     ARDRRIVVTAHRDPEIDDPRPDDLYTVGTLAEIVSVERQQGSNIQVVLEGLHRVDIVQ
                     FDNSRPFYCVRAEQAHEDAIPAEEAQALVQVVRELTTRYSETKNKLNADVLEMILQTD
                     DPSHLADLLTTQLISEAPRRQLFLENINPAKRLEQIAVHLTTEVEMADLESRIKNRVR
                     EQIDKNQREYYLREQLKAIHDELSGESGNEIEALRQKINEAGLPADITERLQKEVVRL
                     ERMPTVSAEATVVRNYIDTVLALPWTKRSEDILDLEHAETILDEDHYGLEQVKERIIE
                     FLAVRKLTDEQELPSMGSTILCLAGPPGVGKTSLGRSIARSMGREFVRVSLGGVRDEA
                     EIRGHRRTYIGAFPGRIVGAMKTAGTMNPVILLDEIDKMSSDYRGDPASAMLEVLDPE
                     QNKEFTDHFLDMSYDLSQVLFITTANYLGNVPRPLRDRMEIIELTGYTEHEKMGIGKR
                     HLLPKQLAAHGLDTKLIDLPEKVWFRLIREYTREAGVRGLERQLATLCRKVARDVVKN
                     QEKGKDAQSKVRVTEAKLEEYLGPKRFGFEQAIGEAQVGVSIGLGTTEVGGELIPVEV
                     AVMPGRGGLTITGRAGDVMQESARAALSYARSRAEQLNIAKDFQEKIDLHIHLPEGAT
                     PKDGPSAGITMATALISAVTRRPVRNDIAMTGEITLRGRVLPIGGLKDKTLAAHRAGI
                     RRLIAPRDNQRDIIKVPEKIREEMEFNFVESMDQVIELALMSNDVVEVVPATVSEPAV
                     EGDETVQIPIEEPATPLVVDIQADA"
BASE COUNT          424 a          878 c          895 g          526 t
ORIGIN      
        1 cgaaccagcc gtcgagggcg acgaaacggt ccagatccca atcgacgagc cggccacccc
       61 gctggtcgtc gatatccagg ccgacgcgta ggccacctac aacacgaaat cagcagccgg
      121 ggcgagtcga tgactcgccc cggctgctga tttcgtgttg taggtggcct acgcgtcggc
      181 ctggatatcc acgaccagcg gggtggccgg ctcttcgatc gggatctgga ccgtttcgtc
      241 gccctcgacg gctggttcgc tgacggtggc cggaacgacc tcgacgacgt cgttggacat
      301 cagcgccagc tcgatgacct gatccatgct ctcgacgaag ttgaactcca tctcctcgcg
      361 gatcttctcc ggcaccttga tgatgtcacg ctggttgtcg cgcggggcga tcaaccggcg
      421 gataccagct cggtgggcgg ccagcgtctt gtctttgagg ccgccgatcg gcagcacccg
      481 gccacgcagc gtaatctcgc cggtcatcgc gatgtcgtta cggaccgggc ggcgggtgac
      541 ggccgagatg agcgccgtcg ccatcgtgat gccggcgctg gggccgtcct tcggtgtcgc
      601 gccttccggc agatggatgt ggaggtcgat cttctcctgg aagtccttgg cgatgttgag
      661 ctgttcggcc cgcgagcgcg cgtaggagag ggcggcccgc gccgactcct gcatgacatc
      721 gcccgcccgg ccggtgatcg tcagcccgcc acggcccggc atgacggcca cctcgaccgg
      781 gatcagctcg ccgccgacct cggttgtgcc gaggccgatc gagacgccga cctgggcctc
      841 gccaatcgcc tgctcgaagc cgaagcgctt cgggccgagg tattcctcca gcttggcctc
      901 ggtgacgcga accttgctct gcgcgtcctt gcccttttcc tggttcttga cgacatcgcg
      961 ggcgaccttg cggcagagcg ttgccaactg gcgttcgagg ccacggacac cggcctcgcg
     1021 ggtgtactca cggatcagcc ggaaccagac cttctccggc aggtcgatca gcttggtgtc
     1081 gaggccgtgg gcggccagct gcttgggcag caggtggcgc ttgccaatgc ccatcttctc
     1141 gtgctcggtg tagccggtca gctcgatgat ctccatccgg tcgcgcagcg gccgcgggac
     1201 gttgccgaga tagttggcgg tcgtgatgaa gagcacctgc gagaggtcgt aggacatatc
     1261 gaggaagtgg tcggtgaatt ccttgttctg ctccggatcg agcacttcca gcatcgccga
     1321 ggccgggtcg ccacgatagt cggacgacat cttatcgatc tcgtccagca ggatgaccgg
     1381 gttcatcgtg ccggccgtct tcatcgcgcc gacgatccgg cccgggaagg cgccgatgta
     1441 ggtacgccgg tggccgcgaa tctcagcctc gtcgcggacg ccgcccagcg agacacgcac
     1501 gaactcgcgc cccatcgagc gggcgatcga gcggccgagg ctggtcttgc cgacacccgg
     1561 agggccggcc aggcagagga tcgtcgagcc catcgacggc agctcctgct cgtcggtcag
     1621 cttgcggacg gccaggaact cgatgatgcg ctccttgacc tgctccagcc cgtagtgatc
     1681 ctcgtcgagg atcgtctcag cgtgctccag gtcgaggata tcctcggagc gcttcgtcca
     1741 cggcagcgcc agaacggtgt cgatgtagtt gcggacgacg gtcgcctcgg ccgagacggt
     1801 cggcatccgc tccagccgga cgacctcctt ctggagccgc tcggtgatgt ccgccggcag
     1861 gccggcctcg ttgatcttct ggcgcagcgc ctcgatctcg ttgccgctct cgccggagag
     1921 ttcgtcgtgg atcgccttga gctgctcgcg caggtagtac tcgcgctggt tcttgtcgat
     1981 ctgctcgcgg acccgattct tgatccgcga ctcgagatcg gccatctcca cctcggtcgt
     2041 caggtggacg gcgatctgct ccagccgctt ggcgggattg atgttttcga ggaagagctg
     2101 ccgccgtggc gcttcggaga tgagctgggt tgtcagcagg tcggccagat ggctcggatc
     2161 gtcggtctga agaatcatct ccagcacatc ggcattgagc ttgttcttcg tctcgctgta
     2221 acgcgttgtc agctcgcgga cgacctgcac cagcgcctgc gcctcttcgg ccgggatcgc
     2281 gtcttcgtgt gcctgctcgg cacggacgca gtagaacggg cgcgagttgt cgaactgcac
     2341 gatgtcgacc cggtggagcc cttcgaggac aacctggatg ttgctgccct gctgtcgctc
     2401 gaccgaaacg atctcagcca gggtcccgac cgtgtacagg tcgtcggggc gcggatcatc
     2461 gatctccggg tcgcggtggg ccgtgacgac gatccgtcgg tcacgtgcca tcgcctcctc
     2521 gaccgcctgg atcgagcggg tccggccgac cagcagcgtc acgacattac ggggaaaaat
     2581 gacgacgttc ttgagtggaa ggagcggata acggactgcg tacaaatcgt accgactcat
     2641 ggggttgaaa ccatcctcac tggtcctgtc attggtcagg acacgtgctg acgctgccgc
     2701 gcacgacgag gaactgtctc cgg
//
LOCUS       DASUGJ010000576         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1760962, whole genome shotgun sequence.
ACCESSION   DASUGJ010000576 DASUGJ010000000
VERSION     DASUGJ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760962"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFV93_13085"
     CDS             <1..222
                     /locus_tag="VFV93_13085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5166328.1"
                     /translation="IGKLKITHRWAGILGYTPDAMPLVGRLPDMPSVYFAAGFNGSGM
                     SYGPLTARLTAEYMLDGTHPGLFHVDRLG"
     gene            267..1427
                     /locus_tag="VFV93_13090"
     CDS             267..1427
                     /locus_tag="VFV93_13090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5166329.1"
                     /translation="MTVSLWQDTAAWPGEIEHERIVADIAIVGGGIVGATLATLLAGA
                     GRDVVVLEAGYVGSGASGRNAGHCIAGLRTNYHRAIEQYGRERAIALRRQMLENREMV
                     REFCDRLAVPYERRGSQYLGIDAAEADGLRACAAALQADGFEAEFSEQDPYDRGFVGR
                     LYQPGDFWLQPWLLVTRLMASSGARVLENCEVRAIEQAGETVTICGRRATVECKQAIL
                     ATNAYSSNLHEYFRDKVFPTRAQMYATEPSTEPRLIEMPTGTEDGFEYFRQLPDGRFL
                     IGGFRNRFTDEEVGYGDETTLQLQAGMQAWVAERFPEVAKLKVTHRWAGIMGFTADAM
                     PLIGRLPDMPNVAFAVGFTGSGMSYGPLAARLTAEYVLDGTHPGLFHVDRQG"
     gene            1482..1712
                     /locus_tag="VFV93_13095"
     CDS             1482..1712
                     /locus_tag="VFV93_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166330.1"
                     /translation="MTAAHNLQSDHDALADDPLSEYMLTNEEFREIFDREAQLRLGIS
                     GDEFIQRWHRGEYPDWDPELTNLVMMLPFLGE"
     gene            1729..2247
                     /locus_tag="VFV93_13100"
     CDS             1729..2247
                     /locus_tag="VFV93_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166331.1"
                     /translation="MERRRIREVLERYRSSSQRAVSCVSAGTVNFQQPPGSRPGEFMF
                     VLNNREPTSISRDLLLQVFFVVDVGVGGGRGDRDFSSSITGYSFTVLHQPNIEVVAYH
                     WHPDSDEGSTETHVHFGPASARLDSAVRPGDLHKVHFPTGFVSIEKFIWLLIDEFKVQ
                     PRRSDWRDILSQ"
     gene            complement(2310..>3022)
                     /locus_tag="VFV93_13105"
     CDS             complement(2310..>3022)
                     /locus_tag="VFV93_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5166332.1"
                     /translation="VVGGSAIMASSVKIREKAMAIAGHKLEVAPGDIEYDRESGSYRV
                     KGSPDQALTFKEIAAAAYAGGLPADIEAGLSSTDFFSTTGETYPFGTHIAMVEIDPDT
                     GQATVTKFWSVDDCGTVISPILVEGQVHGGLAQGIGQALYEEAVYDTDGQLLSATLMD
                     YALPRAENFPMFELHRTVTTTPLNPMGAKGIGEAATIGSTPAVANAVHDALAPHGITH
                     LDMPLTSSKLWAALNDGA"
BASE COUNT          532 a          929 c          998 g          563 t
ORIGIN      
        1 atcggcaagc tgaagattac gcaccgttgg gccggtatcc tcggctacac tcccgacgcg
       61 atgccactgg tcggccggct gccggacatg cccagtgtct atttcgcggc cgggttcaac
      121 ggttccggca tgagctacgg cccgctgacg gcccggctga ccgccgagta catgctcgat
      181 ggcacacacc ccggtctgtt ccatgtggat cggttgggat aacgcagcca accggacgct
      241 gagtgcacga gacgaaggag aagtagatga ccgtctcact ctggcaggac acggctgcct
      301 ggccaggaga aatcgagcac gagcgaattg tggccgacat cgcgatcgtc ggcggcggga
      361 tcgttggcgc gacgctggcg acgttgctgg ctggggccgg acgggatgtc gtcgtccttg
      421 aagccggcta cgttggtagc ggggcgagcg ggcgcaacgc agggcactgt atcgctgggt
      481 tgcgaacgaa ctaccaccgc gctatcgagc agtatggccg cgagcgggcg atcgcgctcc
      541 gccggcagat gctggagaac cgcgagatgg tgcgcgagtt ctgcgaccgg ctcgcggttc
      601 cgtacgagcg gcggggctca cagtatctcg gaatcgacgc tgccgaggca gacgggttgc
      661 gcgcctgtgc tgcggccttg caggccgatg gcttcgaggc cgagttctcc gagcaggatc
      721 catacgaccg tggcttcgtc ggccgtctct accagccggg cgacttctgg ctccagccgt
      781 ggctactggt gacgcggctg atggcgtcct ccggcgcacg ggtgctggag aactgcgagg
      841 ttcgcgcgat cgaacaggcc ggcgagacgg tgacgatctg tgggcggcgt gcgacggtcg
      901 agtgcaagca ggcgatcctg gcgacgaatg cctactccag caacctgcac gagtacttcc
      961 gcgacaaggt cttcccgacc cgcgcccaga tgtacgcgac tgagccgagc accgagccac
     1021 gcctgattga gatgccgacc gggaccgagg acggcttcga gtacttccgc cagctcccgg
     1081 acggccgctt cctgatcggc ggcttccgca atcgcttcac cgatgaggag gtcggctacg
     1141 gcgacgagac gacactccag ctccaggccg gcatgcaggc ctgggtagcg gagcgcttcc
     1201 cggaggtcgc caagctgaag gtgacgcacc gctgggccgg catcatgggc ttcaccgccg
     1261 acgcgatgcc gctgatcggc cggctacccg acatgcccaa cgtcgccttc gccgtcggct
     1321 tcaccggctc cggaatgagc tacggccccc tcgccgcccg cctgaccgcc gagtacgtcc
     1381 tggacggcac ccatccgggt ctgtttcatg tcgatcggca ggggtagtga tgagtcacca
     1441 tggccatcag gtagaatcgt gagactgaaa gcgaggcgat catgacagca gcacacaacc
     1501 tccaatcaga tcacgacgca ctggcagatg atccactgtc ggagtacatg ctgaccaacg
     1561 aggagtttcg ggagatcttc gatcgcgaag cgcaactcag gttgggaatt agtggcgatg
     1621 aatttattca gcgttggcat cgcggtgagt atccagattg ggacccggag ttgaccaatc
     1681 tggtcatgat gctgccgttc ctcggcgaat aacgcgcatc gatcataggt ggaacgacgc
     1741 cgcattcgtg aagtacttga acgctatcga tcctcctctc agcgagcagt ctcatgtgtc
     1801 tcagcaggga ccgtcaattt ccaacagccg ccgggcagcc gtccgggcga gttcatgttc
     1861 gtgctcaaca atcgcgaacc gacttcgatc tctcgcgatc tgttgctgca agtgttcttc
     1921 gttgtggacg taggcgttgg tggaggtcgc ggtgatcgtg acttcagttc gtccatcacc
     1981 ggatacagtt ttacagtgtt acatcagccc aacatcgagg tcgtcgccta ccattggcat
     2041 cctgattcag atgaaggctc cacggaaacg cacgtccact tcggcccagc atcggcgcgt
     2101 ttggattcgg cagtccgccc tggcgacctt cacaaagtcc acttcccgac cggcttcgtt
     2161 tcgattgaaa agttcatctg gctcctgatc gatgagttca aggtccagcc acggcggtcg
     2221 gactggcggg atatcctcag ccagtgagcg tgaatgcaac ggccccgttt ccagaccggg
     2281 aacggggccg ttctcgcagt gaatctcgac tacgcgccgt cgttcagcgc cgcccagagc
     2341 ttgctggacg tgagcggcat gtcgagatgg gtgatgccgt ggggggcgag ggcatcgtgg
     2401 acggcgttgg cgacggcggg ggtggagccg atcgtggcgg cctcgccgat acccttggcg
     2461 cccatcgggt tgagcggcgt cgtcgtgacc gtccggtgca attcgaacat cgggaagttc
     2521 tcggcgcgcg gcagtgcgta gtccatcagc gtcgccgaga ggagctggcc gtcggtatcg
     2581 tagacggctt cctcgtagag cgcctggccg atgccctggg cgaggccacc gtggacctga
     2641 ccttcgacga ggatcgggct gatgacggtg ccgcagtcgt cgaccgacca gaacttcgtc
     2701 actgtcgcct ggccggtgtc ggggtcgatc tcgaccatcg cgatgtgggt gccgaacggg
     2761 taggtctcgc cggtcgtcga gaagaagtcg gtcgaggaca atccggcctc gatgtcggcc
     2821 ggtagcccgc ccgcgtaggc cgccgccgcg atctccttga aggtcagtgc ctggtcgggc
     2881 gagcccttga cgcggtagct gcccgactcc cggtcgtact cgatgtcgcc gggggcaacc
     2941 tccagcttgt ggccggcgat cgccatcgcc ttctcgcgga tcttgaccga cgaggccatg
     3001 atcgccgagc cgccgacgac ca
//
LOCUS       DASUGJ010000577         5925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1761023, whole genome shotgun sequence.
ACCESSION   DASUGJ010000577 DASUGJ010000000
VERSION     DASUGJ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761023"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFV93_13110"
     CDS             complement(<1..284)
                     /locus_tag="VFV93_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166333.1"
                     /translation="MAQESSETFVEAAARAERPVRPELASEPDRAPDIVTYDIVRNDA
                     EVAALVERANENLGVLGYTEHGVRHVTLVSRIARNVLRHLGYDDEHQELVS"
     gene            complement(290..868)
                     /locus_tag="VFV93_13115"
     CDS             complement(290..868)
                     /locus_tag="VFV93_13115"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5166334.1"
                     /translation="MAIHELLAQRWSSRRFAPDPLPDATIETLIEAARWAPSYGNRQP
                     WRFVVAREPATLAALHGTLTRGNAYATVAPVLIALCGNPDNGQIKDGKQYYLMDAGMA
                     LENLLLQAVGLGLHAHPMGGFDEALVRDVLGIPETVHVLALIAVGRPGRLEDLDERTR
                     ERELRERTRKPAGAIRADERWAFGEDIDEGKK"
     gene            complement(874..1998)
                     /locus_tag="VFV93_13120"
     CDS             complement(874..1998)
                     /locus_tag="VFV93_13120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5166335.1"
                     /translation="MVAGSDVVVIGAGVEGSSIAYQCARRGMSVTIVEAREVASQASG
                     ASAGGVRQQGRDPRELPVALRAIGMWPSLTDELQADLEYYQQGHLTMVADETDLPAME
                     ASVARQREGGLDVRMLYGDDLREIVPLASPEMIAGAYCPTDGHANPIATTKAFAAAAV
                     RHGARLLDWTPVTRIVTDSGRAKGVETAKGVIEAGIVIVAAGAWSAALCETAGITIPV
                     NTRAPQMMLTTRMPHALDPVVGCVGRALSLKQLRYGQYLIGGGWPATVYLDVERPIGR
                     NQHNSISGSAWASTSVWPVLRHTQIVRVWAGLEAEAEDLVPVLGATPVENMLIATGFS
                     GHGFALAPYIGVLLAELAATGTTPISLTGLTLDRFAAAGA"
     gene            complement(2008..2766)
                     /locus_tag="VFV93_13125"
     CDS             complement(2008..2766)
                     /locus_tag="VFV93_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5166336.1"
                     /translation="MRIALVQMRCEKGDVVGNLSRIAVEIEAAAARGASLVVFPEMSI
                     TGYIDPARSPEAFLDLESAPVREFVALTADRDLTAIAGIVERNPAGKPYITQLVASRG
                     RLAGIYRKNTVVDEEAEWFAPGDGVPVFEHRGVPFGLAICADIDNPGVFAALARQGAR
                     VVLECAAPGLYGEQATRNWQTGYDWWREKCADQLGAYAREHRISIAVATQAGRTIDED
                     FPGGGYLFGPDGATLAATDDWSEGVLDVEVPVGE"
     gene            complement(2901..4727)
                     /locus_tag="VFV93_13130"
     CDS             complement(2901..4727)
                     /locus_tag="VFV93_13130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX5166337.1"
                     /translation="MTGATGATGALPARSELDPAYTWNLESIFPSDAAWESRFAELAG
                     RLDELDSLRDSIGASAETFLAALRRRDALLLEIEHVRQYAGLRAAEDQTNAHYVAMQD
                     RANGLTSRANAAVAWYEPALLALGEQAIATYLDDNTELAVYGHYFETLFRRQAHVRSQ
                     EVEQVLALADEPLDGFDATWRALNNADLTFEPFEDEAGQTTRLQQNGQYRYLLSSDRR
                     VRQAVWERYYDAYLRLRNTFAANYAGEVKRSVFNARARGHASALDAALAGPNLPPAIY
                     YNLLDALASNVGVWQRFFRVQAKLLGVDRLHGWDLSEQPLELPGRSTRRFSFDEAVAL
                     ILDALAPMGEEYVGIVRQGIADRWVDVYSNVGKTGGAFSSGAPGTHPFILLNFMGQLG
                     TTSTLAHELGHSMHSYYAWRAQPAVYAWYGDVVGETASNLHQVLLADHIMKHSDDPDL
                     QLEVIQERMGGHLRYLFNMVLLARFELDCHQQVERGEALTADEMIATMADLYADAYGD
                     SVVLDRERTGIRWATLPHLFQPFYVYSYGAGTAAAYAIGTQIIEAGQPAAERALAMFA
                     AGDSIDQHEALRLGGADLRDPAVYQSAFDVLAGYVDRLEDLL"
     gene            complement(4849..5853)
                     /locus_tag="VFV93_13135"
     CDS             complement(4849..5853)
                     /locus_tag="VFV93_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5166338.1"
                     /translation="MAKQLGIDLGTANVLVFVRGRGIVINEPSVVAISARDGKVKAVG
                     LEARNMLGREPRETIEVVRPMRDGVIADYVVTQEMLHYFINKAVGKFSLTKPDVMICV
                     PAGVTGVERRAVRDAALAAGARRAYLISEPLAAAIGAKVPVADPSGNMVIDIGGGTTE
                     VAVISLNGIVKANSIRVGGNRFDEAIANYIKRKYNLRIGERTAEEVKIAIGSAMPLDE
                     DMTMDVRGRDEVAGLPRTIRIHSNEVVEAITEPLEAIIGAVRAVLEETPPELSSDIID
                     KGMILTGGGALLRGLSDLLTEVTGVPCYVADDPLSCVAVGTGLALEYFDILRDSLEEP
                     "
BASE COUNT          992 a         2015 c         1947 g          971 t
ORIGIN      
        1 gataccagct cctgatgctc gtcgtcgtag cccagatggc gcagcacgtt gcgggcgatc
       61 cgtgagacga gcgtgacgtg gcgaacgcca tgctccgtgt agccgaggac acccaggttc
      121 tcgttggcgc gctcgaccag cgcagcgacc tcggcgtcgt tgcgaacgat gtcgtaggtg
      181 acgatatccg gagcgcgatc cggctcggac gcaagttcgg gccggaccgg gcgttcggca
      241 cgggcagccg cctcgacgaa cgtttcagat gattcctgag ccatcgctgc tacttctttc
      301 cctcgtcgat atcctcgccg aacgcccaac gctcgtcagc gcgaatcgcg ccggccggct
      361 tgcgcgtccg ctcgcgtagc tcgcgctcgc gcgtgcgctc gtccaggtcc tccaggcgcc
      421 ccggccggcc aacggcgatc agcgccagga catggaccgt ctcgggtatc cccagcacgt
      481 cgcgcaccag ggcctcgtcg aagccaccca tcggatgcgc gtgcagtccc agcccgacag
      541 cctggagcag caggttctcc agcgccatcc cggcgtccat caggtaatac tgcttgccgt
      601 ccttgatctg gccgttgtcg ggattgccac agagggcgat caggaccggc gcgaccgtcg
      661 cgtaggcgtt gccgcgcgtc agagtgccat gcagagccgc cagcgtcgcc ggctcgcggg
      721 cgacgacgaa gcgccacggc tggcggttgc cgtacgacgg cgcccagcga gccgcctcga
      781 tcagcgtctc gatcgttgca tcgggcagcg gatcgggcgc aaaacggcgg ctgctccaac
      841 gctgggcaag caactcgtga atcgccatgg cggctacgct ccggctgccg caaagcggtc
      901 gagagtcagg ccagttagcg agatcggcgt cgtgccggtc gccgccagtt cggccagcag
      961 gaccccgatg tacggcgcga gggcgaagcc gtggccggag aagccggtcg cgatcagcat
     1021 gttctcgacc ggcgtcgcgc ccaggacagg cacgaggtct tccgcctcgg cttctaaccc
     1081 ggcccagaca cgcacgatct gggtgtggcg caggaccggc cagacggagg tcgaagccca
     1141 ggcgctgccg gagatgctgt tgtgctggtt gcggccgatc ggtcgctcga catcgaggta
     1201 gacggtggcg ggccagccgc cgccgatcag gtactggccg tagcgcagtt gcttgaggct
     1261 gagtgcccgc ccgacgcagc cgacgaccgg atcgagcgcg tgtggcatcc gcgtcgtcag
     1321 catcatctgc ggggcgcggg tattgaccgg aatcgtgatg ccggcggtct cgcacaacgc
     1381 cgcgctccag gctccggccg cgacgatgac gatcccggcc tcgatgaccc ctttggcagt
     1441 ctcgacgccc ttcgcgcggc ccgaatcagt cacgatccgc gtgaccgggg tccagtcgag
     1501 cagccgcgcg ccgtgccgga cggctgcagc cgcgaacgcc ttcgtcgtcg cgatcgggtt
     1561 ggcgtggccg tcggtcgggc agtaggcgcc ggcgatcatc tccggcgagg cgagcggcac
     1621 gatctcgcgc aggtcgtcgc cgtagagcat ccgcacatcc agcccgccct cgcgctggcg
     1681 ggccacgctc gcctccatcg ctggcaggtc ggtctcgtca gcgaccatcg tcaggtggcc
     1741 ctgctgatag tactccaggt cggcctgtaa ctcgtcggtc agcgacggcc acatcccaat
     1801 cgcgcgcagg gcgaccggta gctcgcgcgg atcgcgtccc tgctggcgca cgccaccggc
     1861 gctggcgccc gacgcctggc tggcgacctc gcgcgcctca acgatcgtga cgctcatccc
     1921 acgccgggcg cactggtagg cgatcgagct cccttcgacg cccgcgccaa tgacgacgac
     1981 atcactgcct gcgaccacgc tcactcctca ctccccgacc ggcacctcga catccaggac
     2041 gccctccgac cagtcgtcgg ttgctgcgag ggtagcaccg tccggcccga acaggtagcc
     2101 gccaccgggg aaatcctcgt caatcgtccg tcccgcctgg gtcgcgacgg cgatcgagat
     2161 ccgatgctcc cgcgcgtagg cgcccagctg atcggcgcat ttctccctcc accagtcgta
     2221 gccggtctgc cagttgcgcg tcgcctgctc gccgtacagc cccggcgcgg cgcattcgag
     2281 gacaacgcga gcgccctgcc gggcaagcgc ggcgaagaca cccggattgt cgatatcagc
     2341 gcaaatggca agcccgaacg ggacgcctcg atgctcgaag accggcacgc cgtcacccgg
     2401 agcgaaccac tccgcctctt cgtcaacgac cgtgttcttg cgatagatgc cagccagtct
     2461 tccgcgactg gcaacaagct gagtgatgta cggtttgccg gccggattgc gctcgacgat
     2521 cccggcaatc gcagtcaggt cgcgatcagc cgtcagcgcg acgaattcac ggaccggcgc
     2581 gctttccaga tcgaggaaag cctccggcga tcgtgccgga tcgatatagc cggtgatgct
     2641 catctccgga aagacaacaa gcgaggcgcc ccgcgcagcc gccgcctcga tctctaccgc
     2701 gatccgcgag agattcccga caacatcccc cttctcgcat cgcatctgaa cgagcgcgat
     2761 tcgcattggt ttaagtccac tcattgatgc tatcaacggt gctgagttca ggacgaacag
     2821 gttcctccgc tttgtcatcc tgaggctgcc gcctcaggat gacaaacggg acggatgcga
     2881 atcctctcac gtgtcgatcc tcacaataga tcctccagcc ggtcaacgta gccggcgagg
     2941 acatcaaagg ccgattggta gacggccggg tcgcgcaggt cggcgccgcc gaggcggagg
     3001 gcctcgtgct ggtcgatgct gtcgccggcc gcgaacatgg cgagcgcgcg ttcggcggcc
     3061 ggctggcccg cttcgatgat ctgcgtgccg atcgcgtagg cggcggccgt gccggcgccg
     3121 taggagtaga cgtagaacgg ctggaacagg tgcggcagcg tcgcccagcg gatgccggtc
     3181 cgttcgcggt cgaggacgac gctgtcgccg tacgcatcgg cgtagaggtc ggccatcgtc
     3241 gcgatcatct cgtcggccgt caacgcctcg ccccgctcaa cctgctggtg gcagtcgagc
     3301 tcaaagcgcg ccagcaggac catgttgaac aggtagcgca ggtgcccgcc catccgctcc
     3361 tggatcacct cgagctgaag atccgggtcg tcggagtgct tcatgatgtg atcagccagc
     3421 aggacctggt gcaggttcga ggccgtttca ccaacgacgt cgccgtacca ggcatagacg
     3481 gctggttgcg cccgccaggc atagtacgag tgcatcgagt gaccaagctc gtgggccagc
     3541 gtcgaggtgg taccgagctg gcccatgaag ttcagcagga tgaacggatg cgtgcccggc
     3601 gcaccgctgg agaacgcgcc gcccgtcttg ccgacgttcg agtagacgtc gacccagcga
     3661 tccgcgatcc cctgccggac gatgccgacg tactcctcgc ccatcggcgc gagtgcgtcg
     3721 aggatcaagg caaccgcctc gtcgaagctg aaccgtcgcg tcgatcggcc aggcagttcc
     3781 agtggttgct ccgagagatc ccagccatgc agccggtcga caccgagcag cttcgcctgc
     3841 acccggaaga agcgctgcca gacgccgacg ttgcttgcca gcgcgtcaag caggttgtag
     3901 tagatcgcag gcggcaggtt cggcccggcg agcgccgcgt cgagggccga agcatggcca
     3961 cgggcacggg cgttgaagac gctgcgcttg acctcaccgg cgtagttcgc ggcgaacgtg
     4021 ttccgtagcc gcaggtaggc gtcgtagtaa cgctcccaga ccgcctggcg aacgcgccgg
     4081 tcggacgaca gcagatagcg gtactgcccg ttctgttgca gccgggtcgt ctgcccggcc
     4141 tcgtcctcga acggctcgaa ggtcaggtcg gcgttgttca gcgccctcca ggtggcgtcg
     4201 aacccgtcca gcggctcgtc ggccagtgcc agcacctgct cgacctcctg ggaccggaca
     4261 tgtgcctgcc gtcgaaacag cgtctcgaaa tagtgtccat acacggcaag ctcagtgttg
     4321 tcgtcgaggt acgttgcgat cgcctgctca ccgagggcca gcagcgcagg ctcgtaccag
     4381 gcaacggcag cgttcgcgcg ggacgtcagc ccattcgcgc gatcctgcat cgcgacatag
     4441 tgggcgttgg tctggtcttc agcggcgcgc agcccggcgt actggcgaac gtgctcgatc
     4501 tcaagcagga gcgcgtcgcg gcgccgcagc gcggccagaa acgtctcagc cgacgccccg
     4561 atcgagtcgc gcaggctgtc cagctcgtca agccgtccgg ccaactcagc aaagcgcgac
     4621 tcccaggcgg catcgcttgg gaagatgctc tccaggttcc aggtgtaggc agggtccagt
     4681 tcagatcggg ccggcaacgc accggtcgcc ccggtcgctc ctgtcatcgt ctctactctc
     4741 accgagccgg tagccatcta cgcgaccacc ggcagccgac gctcaggctg gagggccgtc
     4801 caaaccgtca cggcggtgag tgtagacgaa gatcggaagc cccgctggct atggctcttc
     4861 cagcgagtcg cgcaggatgt cgaagtattc gagggcgagg ccggtgccga ctgccacgca
     4921 ggagagcgga tcgtcggcga cgtaacacgg gacgccggtc acctcggtga gcagatcgga
     4981 gaggccacgg agaagcgcgc cgccgccggt caggatcatg cccttgtcga tgatgtcgga
     5041 cgagagctcg ggcggcgtct cttcaaggac ggcccggacg gcgccgatga tcgcctccag
     5101 cggctcggtg atcgcttcaa cgacttcatt ggagtggatg cggatcgtcc gtggcaagcc
     5161 ggcgacctcg tcgcggcccc ggacgtccat cgtcatgtcc tcgtcgagcg gcatcgccga
     5221 gccgatcgcg atcttgacct cttcggccgt ccgctcaccg attcgcaggt tgtacttgcg
     5281 cttgatgtag ttggcgatcg cctcgtcgaa acggttgccg ccgacacgga tcgagttggc
     5341 cttgacgatg ccgttgagcg agatgacagc cacctcggtc gtcccgccgc cgatgtcgat
     5401 gaccatattg ccgcttgggt ccgcgaccgg caccttcgcg ccgatggcag ccgccagcgg
     5461 ctcggagatc aggtaggctc gccgagcgcc ggccgccagg gcagcgtcgc gaactgctcg
     5521 ccgctcgacg ccggtgacgc cggccgggac gcagatcatc acgtccggtt tggtcagcga
     5581 gaacttgccg acggccttgt tgatgaagta gtgcagcatc tcctgggtga cgacgtaatc
     5641 ggcgatcacg ccgtcgcgca tgggccggac aacctcgatc gtctcgcgcg gttcgcggcc
     5701 gagcatgttg cgcgcctcga gaccgaccgc cttgaccttg ccgtcgcgag cggagatggc
     5761 aacgaccgac ggctcgttga tgacaatccc gcgcccgcgc acgaagacga gcacgttggc
     5821 cgtcccgagg tcgataccaa gctgctttgc caagctggct ccaggatcca gaactggtca
     5881 cataggtgta cggacacccg gaacgggcgt ccgtacaggc gaagt
//
LOCUS       DASUGJ010000578         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_372099, whole genome shotgun sequence.
ACCESSION   DASUGJ010000578 DASUGJ010000000
VERSION     DASUGJ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372099"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..757)
                     /locus_tag="VFV93_13140"
     CDS             complement(62..757)
                     /locus_tag="VFV93_13140"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5166339.1"
                     /translation="MRTHPAGWEHVPQPFAVAHRAGNSLAGAADAVAAGADILEADVW
                     LYRGRLEVRHEKTLGPLPILWDRWSLSTGRQRLDLASLLDAVDPEIELLLDLKGYPGS
                     DPAIAARIAQTMDRQRPGRPFLVCSQNWRLLEAFRPFPAALLVHSIGNQRQLTRAWRR
                     LEQDDHEAVSIQVRLLNAEIVRALKQRVATLFTWPINDTGQVQTVLDWGVDGVTTDRL
                     AIVEQVRAIRDRR"
     gene            complement(754..1626)
                     /locus_tag="VFV93_13145"
     CDS             complement(754..1626)
                     /locus_tag="VFV93_13145"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5166340.1"
                     /translation="MSDVPGIEPLFFLFSIDWNPLNWQSWPFIVIIVAALAVRFFLPP
                     VTTSKQALLAEITLLLPAGLLYFLVRGLVDARADEAIANAWQVIDFERAVGISLEPEM
                     QKALLRSETLETAVNWIYIWMHWPVVIGTMIWLIARRPHERYRVYRNAFLLSGAIGMC
                     IFAIHPVAPPRLVPGLGLEDTVTLHSHSYRVLQPPALTNPYAAMPSLHFGWNLLFGIA
                     LVRESRKRLVRGFGFVMPLLMYIAIVLTANHYVVDGLVGGVLTATCLLVALKIPAETG
                     QWLTWHGLRERWRL"
     gene            complement(1681..2955)
                     /locus_tag="VFV93_13150"
     CDS             complement(1681..2955)
                     /locus_tag="VFV93_13150"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEX5166341.1"
                     /translation="MVVSRSFAIFGSVRAALGRHAILLWTILVLLGLGLFAYQQQHEA
                     GELAATFASAAPGWLLVLVAFQLAGIGCSVLVYRGVLKRLGHCVCPGRLALIHLQRHV
                     VSVLTPIGGPASAYVFVRGLASHQVSTNDSLLTLALRGVSGYAAFVTLLIPALLLTTP
                     STLMLVAAVMLALLLVVALVALFVVMRGDASSEHLPGWLPERAAGFVVQCRSHALGPR
                     DLVYPYVVALQLNLCGVAMLYASLQALGQQVSPATALAAFAVGNLFAIVAPVFQGVGV
                     VEVSMAVTLQGFGVPGSIALGATLLFRVADVWFPLALGLLAQAGQHPPVRRTGSRLAG
                     LTAGAGIVFLSWYAALAGAGLRDYQVALLTLLAGAAGLLMAYAPWRRLLVARYTTAGI
                     ALGAVPVIFDGQLDHLALLMAQAGMALSVLIR"
     gene            complement(3209..3616)
                     /locus_tag="VFV93_13155"
     CDS             complement(3209..3616)
                     /locus_tag="VFV93_13155"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5166342.1"
                     /translation="MAADENRELARRFFEEVWNERDEAAIDRYIAADAAGNDPTFGAG
                     REAFRRQWRQWQAAFPDLHFAVEEIVAEGDVVVTRWTLTGTHSGADFEGTPASGATIK
                     VGGMSLDHFRDGMIAAGFDGWDELGFRRQLGVL"
     gene            3718..>3969
                     /locus_tag="VFV93_13160"
     CDS             3718..>3969
                     /locus_tag="VFV93_13160"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5166343.1"
                     /translation="MTREERLAELGFELPVIEVGDRPLIPWVQVGNLLYTSGRTPSAS
                     HKTGQVGADVTVDEGYQAAREIAAGQLAVAKTALGDLSRI"
BASE COUNT          783 a         1390 c         1250 g          546 t
ORIGIN      
        1 gtccgcgatg gcaaggtcgc cgagaagctg gcttacgtca agggctagcg ccacgacgcc
       61 actagcgtcg atcgcggatt gcccgaacct gctcgacgat cgcgagccgg tcggttgtca
      121 cgccatcgac gccccaatcc agcacggtct gcacctggcc ggtgtcgttg atcggccagg
      181 tgaagagcgt cgccacgcgc tgcttgaggg cacgcacgat ctcagcgttc aacaaccgca
      241 cctggatgct gacggcctcg tggtcatcct gctccagccg ccgccaggcg cgtgttagct
      301 gccgctggtt gccgatcgag tggaccagga gggctgctgg aaatgggcgg aaggcctcca
      361 gcagacgcca gttctggcta cagaccagga acggccggcc gggacgctgc ctgtccatcg
      421 tctgggcgat tcgggcggcg atggccgggt cactgccggg ataccccttc aggtccaaca
      481 gtaactcgat ctcaggatcg acggcgtcga gcaggctcgc cagatccagc cgctgccgcc
      541 cggtcgagag cgaccagcga tcccagagga tcggcagcgg cccgagtgtc ttctcgtggc
      601 gcacctccag ccgcccgcga tagagccaga catcagcctc caggatatcg gcgccggccg
      661 cgaccgcatc ggcggccccg gcgaggctgt tgccggcccg gtgggcaacc gcgaatggct
      721 gtgggacatg ctcccagccg gccgggtgcg tcctcacagg cgccaccgct ccctgagtcc
      781 gtgccatgtc agccattggc cggtctcggc cggaatcttc aacgccacca gcaggcaggt
      841 cgccgtcagc acaccaccga ccagcccatc cacgacgtag tggttggccg tcaggacaat
      901 cgcgatgtac atcagcagcg gcatgacgaa gccaaaacca cgcaccagtc gcttgcgcga
      961 ctcacgcacg agcgcgatgc cgaacagcag gttccagccg aaatggagac tcggcatcgc
     1021 cgcgtacgga ttcgtcagcg ccggtggctg gaggacgcgg tatgagtgcg aatggagcgt
     1081 gaccgtgtcc tccaggccga ggccgggcac gagcctgggt ggcgccaccg ggtgaatggc
     1141 gaagatgcac atcccgatcg cgcccgacag caggaaggca ttgcgataga cccggtagcg
     1201 ctcgtgcggg cgacgggcga tcagccagat catcgtcccg atcacgaccg gccagtgcat
     1261 ccagatgtag atccagttga cagccgtctc gagcgtttcc gaacgcagca aggctttctg
     1321 catttccggt tcgagggaga tcccgacggc ccgctcgaag tcgattacct gccaggcatt
     1381 cgcgatcgcc tcgtccgcgc gggcatcgac cagcccccgg acgaggaaat agagcaatcc
     1441 ggccggcagc agcagcgtga tctcggccag caacgcctgt ttggatgtcg tgacgggtgg
     1501 cagaaagaag cgaacagcca gggcagcgac gatgatgacg atgaacggcc agctttgcca
     1561 gttgaggggg ttccagtcga tcgagaagag gaaaaagaga ggctcaattc cgggaacatc
     1621 ggacatgcag aacggctccg ctccagtctc ggtcaacgag cgccgttcgc cgccgcaata
     1681 tcaccgaatc agcacagaga gggccattcc ggcctgagcc atcagcaacg ccaggtggtc
     1741 cagttgcccg tcgaagatga ccggaacggc gccgagtgcg atgcctgccg tcgtgtatcg
     1801 cgccaccagc aaacgccgcc acggagcata cgccatcagc aggccggcag caccggctag
     1861 cagcgtcagc agcgcaacct ggtagtcccg caacccggcc ccggccagcg ccgcatacca
     1921 gctcaggaat acgataccgg ctccggccgt cagtccggcc aggcgcgatc cagtgcgccg
     1981 gaccggcggg tgctgaccgg cctgcgccag cagccccagc gccaacggga accagacatc
     2041 ggcgacgcga aacagcagcg tcgccccgag cgcaatcgac cccggaacac caaagccctg
     2101 taaggtgacg gccatgctga cctcgacgac gccaacgccc tggaagacgg gcgcgacaat
     2161 cgcgaacaga ttgccgaccg cgaaggccgc cagcgccgtc gccgggctga cctgctgccc
     2221 aagcgcctgg aggctcgcgt acagcatcgc gacaccacac aggttcagtt gcagcgccac
     2281 gacatacgga tagaccaggt cgcgcgggcc tagcgcatgc gagcgacact ggacaacgaa
     2341 gccggcggcc cgttccggca gccagcccgg caggtgctcg ctcgacgcat cgccacgcat
     2401 gacgacgaac agcgccacca gcgcgacaac cagcaggagc gcgagcatga ctgccgccac
     2461 gagcatcaac gtcgacggcg tcgtcagcag cagcgccggg atcagcaacg tcacgaacgc
     2521 ggcgtagcca ctcacgccac gcagcgcgag cgtcagcagg ctgtcgttgg tcgacacctg
     2581 atgactcgcc agccctcgca cgaagacata ggccgaagcc ggcccaccga tcggcgtcag
     2641 gacactgacg acatgtcgct ggaggtggat cagcgccagc cgtccggggc agacacagtg
     2701 ccccaggcgc ttgagcaccc cgcggtagac gaggaccgaa cagccgatcc ccgccagttg
     2761 gaaggccacc aataccagca gccagccggg cgctgcgctg gcgaacgtcg ctgccagttc
     2821 cccggcctca tgctgctgct gatacgcgaa caggccaaga cccagcagga ccaggatcgt
     2881 ccagagcagt atcgcatggc gtccgagcgc cgcccggacg ctgccgaaga tcgcgaatga
     2941 acgggaaacg accactgatt caacaatccc tgttacaccg gcggcgggct gcaccccacc
     3001 tgatccggca cctgacccca ggtttaacgg tgataacagg gcgaaacacc aaccggcgcc
     3061 tgaccgcagg caaccggaac cacgtcacgc tccctgaccc gtaaccccag cttcgatctc
     3121 tcgatcgcac gcatcctcgc acaaaggata cccgcgattg tcattctatc gacacgtcgg
     3181 cggattcgca tcacgcgaag gtcacgattc agaggacgcc gagttgccgc cggaacccca
     3241 actcgtccca gccgtcgaag ccggcggcga tcatgccgtc gcggaagtgg tcgaggctca
     3301 tcccgccgac cttgatcgtc gcgccgctgg cgggcgtacc ctcgaagtcg gcgccggagt
     3361 gtgtgccggt cagcgtccag cgcgtcacga cgacatcgcc ctcggcgacg atctcctcga
     3421 cggcgaagtg caggtcgggg aaggccgcct gccactgtcg ccactgcctg cgaaacgcct
     3481 cgcgccccgc cccgaaggtc ggatcattcc cggccgcgtc ggccgcaatg tagcggtcga
     3541 tcgccgcctc gtcccgctcg ttccagacct cctcgaagaa ccgccgcgcc aactcccgat
     3601 tctcgtccgc cgccatccgc gctccccctc cgtcatccca cgtcccgcat cccgcgtccc
     3661 gaaacccgga cgatggtaga gtgatgcgag tctgttcgca acaagggagg ggaacgcatg
     3721 acacgcgaag agcgcctggc cgagcttggc ttcgagttgc cggtgatcga ggtcggcgac
     3781 cggccgctga tcccgtgggt ccaggtcggc aacctgctct acacctccgg ccggacgccg
     3841 agcgcgtccc acaagaccgg ccaggtcggg gccgacgtga ccgttgacga ggggtatcag
     3901 gccgcccgcg agatcgccgc cggccagttg gcggtcgcca agaccgccct cggtgacctg
     3961 agccggatc
//
LOCUS       DASUGJ010000579         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1205445, whole genome shotgun sequence.
ACCESSION   DASUGJ010000579 DASUGJ010000000
VERSION     DASUGJ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205445"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..2158
                     /gene="lepA"
                     /locus_tag="VFV93_13165"
     CDS             332..2158
                     /gene="lepA"
                     /locus_tag="VFV93_13165"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX5166344.1"
                     /translation="MTDQKHIRNFSIIAHVDHGKSTLADRLLEYTGTLTSRQMMEQVL
                     DTMDLEREKGITIKARAVRMDFRAADGELYELNLIDTPGHVDFSYEVSRSLAACEGAL
                     LVVDAAQGIEAQTIANVYLALENDLALIAVINKIDLPNAMPEKVSNEVGDLIGLLPDE
                     IIPASAKEGKGVPEILQALVDRIPPPAGTEKAPLRALIFDSHYDAYKGVIAYVKIVDG
                     VLETDSRIRVMATGKEAELLEIGVFRPNMTPVKRLGTGEVGYVATGLKVVRDCQVGDT
                     ITLAASPASDPVPGYRPVKPMVFAGFFPVNNEDYTELRDALDRLQLNDASLVFEPESS
                     DALGFGFRCGFLGLLHMEIIQERLEREYNLDLLATAPSVEYEVLKMTGETILVDNPSD
                     LPDPGVIEEIREPWMDITIISPARYIGTVMDLITNRRGNFDSMDYLDEDRVSLKFGMP
                     LGELIVDFYDQLKSRTQGYASLDYAFHSMRPADLVKLDVLVNGVPVDALSMITHRGDA
                     YYRGRALVQQLRKLIPRQMFEVPIQAAVGSRIISRETIAAMRKNVLSKCYGGDITRKR
                     KLLEKQKEGKARMKMVGNVEIPQEAFMAILTLGDERAVAERS"
BASE COUNT          423 a          717 c          661 g          390 t
ORIGIN      
        1 ctcttcgtcg gcctgcgcga tcacttgctc ggccgaccgc accagcgttc ggctggctga
       61 ccgaaaacgg cggtttcgaa tgcggcgccg ctcgtttgtc ctggcgcgct tctcggcaga
      121 cttggaattg gccaacgctt atttctccct cgacctgcgt gaacatgaca aaagcgcccg
      181 accatcgcgg gcgtggcaac cttgctattc tacgcgaatc cgcgccacaa tcgcaacatc
      241 gcctggcaca agtcgggatg ccgactccag tatacttact cacttcgggc aggcaacgat
      301 gcccgctcgc ttcagcatgc attgagaaca gatgactgac cagaaacaca ttcgcaattt
      361 cagcatcatc gcccatgtcg atcacggcaa gtccacgctg gccgatcgcc tgctcgaata
      421 caccggcacg ctcaccagcc ggcagatgat ggagcaggtg ctcgacacga tggacctcga
      481 acgtgagaag ggcatcacaa tcaaggcccg tgccgttcgg atggacttcc gcgcggccga
      541 cggcgagctc tacgagctaa acctgatcga cacgcccggc cacgtcgact tctcctacga
      601 ggtcagtcgc tcgctggccg cctgtgaggg cgccttgctg gtcgtcgacg ccgcccaggg
      661 aatcgaggcc cagacgatcg ccaacgtcta cctcgcgctg gagaacgacc ttgcgctgat
      721 cgccgtcatc aacaagatcg acctgccgaa cgcgatgccg gagaaggtct ccaatgaagt
      781 cggtgacctg atcggcctcc tgccggatga gatcatcccg gcctccgcca aggagggcaa
      841 gggcgtcccg gagatcctgc aggcgctagt cgatcggatt ccgccgccgg ccgggacgga
      901 gaaggctccg ctgcgggcgc tgatcttcga ctcgcactac gacgcctaca agggcgtcat
      961 cgcctatgtg aagatcgtcg atggcgtcct ggaaaccgat tcgcggattc gcgtcatggc
     1021 caccggcaag gaggcggagt tgctggagat cggcgtcttc cggccgaata tgacgccggt
     1081 caagcggctc ggcaccggcg aggtcggcta cgtcgcaacc ggactgaagg tcgttcgcga
     1141 ctgccaggtc ggcgatacga tcacgctggc agccagcccg gccagcgatc cggttcccgg
     1201 ctatcgtccc gttaagccga tggtcttcgc cggtttcttc ccagtcaaca acgaggacta
     1261 caccgagctg cgcgacgcac tggaccggtt gcagctcaac gacgcctcgc tcgtcttcga
     1321 gccggaatca tcggacgcgc tcggcttcgg cttccgctgt gggttcctcg ggctgctgca
     1381 catggagatc atccaggagc ggctggagcg cgaatacaac ctcgacctac tggcgacggc
     1441 cccgagcgtc gaatacgaag tcctcaagat gaccggcgaa acgatcctgg ttgacaaccc
     1501 ctccgatctg cccgatcccg gcgtcatcga ggagattcgc gagccgtgga tggacatcac
     1561 gattatttcg ccagcccgct acatcgggac ggtcatggac ctgatcacca accggcgcgg
     1621 taacttcgac tcgatggact acctcgacga ggatcgcgtc tcactcaagt tcgggatgcc
     1681 gctcggtgaa ctgatcgtcg acttctacga ccagctcaag tcgcgcacgc agggctacgc
     1741 ctcgctcgac tacgccttcc acagcatgcg gccggccgac ctggtcaagc tggacgtgct
     1801 ggtcaacggc gtcccggtcg atgcgctctc gatgatcacg caccgtggtg acgcgtacta
     1861 tcgcgggcgg gcgctggtcc agcagctacg caagctcatt ccgcgccaga tgttcgaggt
     1921 gccgatccag gcggccgtcg gcagccggat catctcacgc gagacgatcg cggcgatgcg
     1981 caagaacgtc ctctccaagt gctacggggg tgatatcacc cgcaagcgca agctgctcga
     2041 gaagcagaag gagggcaagg cgcgtatgaa gatggtcggc aacgtcgaga tcccacagga
     2101 ggcgttcatg gcgattctga cgctcggcga cgagcgcgcc gtcgcggaac gcagctgacg
     2161 tgtcccgtcc tatcctcgtc gtcggcctgg c
//
LOCUS       DASUGJ010000580         4855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1205604, whole genome shotgun sequence.
ACCESSION   DASUGJ010000580 DASUGJ010000000
VERSION     DASUGJ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205604"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFV93_13170"
     CDS             complement(<1..211)
                     /locus_tag="VFV93_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166345.1"
                     /translation="MSRSAGQPNFVPHGQDLFGEHHNMGAGVMTFKVTAAESHGALLI
                     VELAHHSAGGPPRHRHPQQDEWFYVT"
     gene            365..769
                     /locus_tag="VFV93_13175"
     CDS             365..769
                     /locus_tag="VFV93_13175"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5166346.1"
                     /translation="MKYLLLKHYRGAPAAVNNVPMDQWAPEEVSAHIQYMHDFATRLE
                     GTGEFVDAQALSPDGTWVRYDGEGRPPVVDGPFAETKDLIAGWMVIDVETQERAIELA
                     GERSAAPGAGGKPIHEWLEVRPFLTGSSTTTE"
     gene            773..1921
                     /locus_tag="VFV93_13180"
     CDS             773..1921
                     /locus_tag="VFV93_13180"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX5166347.1"
                     /translation="MVDEALLRTLTPTVIGILVRRGAPFAAAEDAVQDALIEAVRVWP
                     ANPPQDARSWLVTVAWRKFLDATRSETSRRQREDIVSREPLPEPGEDVDDTLQLYFLC
                     AHPSLTPASAVALTLRAVGGLTTRQIAQAYLVPEATMAQRISRAKRTVSGVRFNQPGD
                     VATVLRVLYLVFNEGYSGDVDLAAEAIRLTRQLAARINHEEVAGLLALMLLHHARRPA
                     RTGPDGSLIPLAEQDRSRWDTRLIAEGIDLLQAALARDRLGEFQAQAAIAALHADAQT
                     AAETDWVQIVEWYDELVRLTDSPIARLNRAVAVGEADGPRAGLAALAELDPSLPRYVA
                     VAAYLHERDGDTPTAARLYADAARLATSIPERDHLTRQASRLNAALRA"
     gene            complement(1934..2788)
                     /locus_tag="VFV93_13185"
     CDS             complement(1934..2788)
                     /locus_tag="VFV93_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166348.1"
                     /translation="MNTLTIARFTLQEAISRRLILAGTILSMAFLGLFALGFFLIYQN
                     DSELSGPLSDAQAQAMFGTVMSVLGLYAINFLAGFLALFLSVGAISSEIDSGALHAVL
                     ARPLRRVEFVAGRWLAYAGLISVYVGVMAGSLLLSAWLIADYTTPDPVRATLLMMLAA
                     IVLLTISLLGSTILSTLANGVVVFSLFGLAWLAGIIEFIGGALSNESMLNIGTAVSLL
                     IPSDAIWRGASYYVLSPLYLAGVQSAEEGMPFVGNVPPATPMVIWALLYPVVLLGAAI
                     LTFSRRDL"
     gene            complement(2785..3771)
                     /locus_tag="VFV93_13190"
     CDS             complement(2785..3771)
                     /locus_tag="VFV93_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166349.1"
                     /translation="MSLHLSTEPIARMAAAAAIPAIETTGLRKVYGRTAVLHDLALSV
                     APGEIFGFLGPNGAGKTTTVKILMGLVRPTAGEARIFGLPAGDRAARRRVGYLPENFR
                     FHDWLTGAGLLDFHGRLAGLSPDERRIRIPEVIGRVGLAGKGDAKIRTYSKGMTQRIG
                     LAQAILHRPDLVLLDEPTSALDPVGRREVRDLIRSLRDDGVTVFLNSHLLSEVEMVCD
                     RVAIIDHGRVVRSGGVSELRVMVDRVDSELLAALKTVADVVEAEEIGADDQHGTPATS
                     HGATLTLAVSDLEIAPLVAETVMRRGYQLFGLVPVHRTLEEIFMDLVEGGES"
     gene            complement(3749..4642)
                     /locus_tag="VFV93_13195"
     CDS             complement(3749..4642)
                     /locus_tag="VFV93_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166350.1"
                     /translation="MTGALPIQRWRIVMSGLAVALVLILILATTAGRGAASDFLAEFR
                     GERFAAVSLSTDQIANVEQTMSEMQHLGTVSGIETAPESQVVAGVAAASQAVGFPVLE
                     PDPATLPEGVGPSPAQVRVVPAHQVRFTVDLDQARAYYQSIGQNDVTLSERFDGASLI
                     INTPPAVVLEYRNAAAPTSSPFGIGLLVGQAGTVTAGSEGGVTLDELRAFLLGLPGLS
                     PETVEQLQEIDSWKTTLPVPIPVDQVNWERATIAGSPGLLLNDNTGLGSAAIWERDGR
                     IYAVAGAMKARELKRVAASLH"
BASE COUNT          829 a         1689 c         1594 g          743 t
ORIGIN      
        1 cggtgacgta gaaccactcg tcctgctgcg ggtgccggtg gcggggtggg ccaccggcgc
       61 tgtggtgggc cagctcgacg atcaacaacg cgccgtgact ctcggctgcc gtaaccttga
      121 aggtcatgac acccgcgccc atgttgtggt gctccccaaa gagatcctgt ccgtgcggaa
      181 cgaagttggg ctgtcctgcg gatcgtgaca tgcgcgcacc tctcggtttc atctgggttg
      241 ccctctgggc gatgttctac gatctatcgt gtggaatgtc gagaaatcgc gcgcggctcc
      301 gtctctacag tgaatgcggc cagaatgggc cgtacgctct tcgggagaac tgaaggagaa
      361 cagaatgaag tacctgttgc tcaagcacta ccgtggcgca ccggcggcgg tcaacaacgt
      421 gccgatggac cagtgggcgc cggaggaagt ctcggcccac atccagtaca tgcacgactt
      481 cgccacccgg cttgagggca ccggcgagtt cgtcgacgcc caggcgctct cgcccgacgg
      541 cacctgggtc cgctacgatg gtgagggacg gccgccggtc gtcgatggcc cgttcgcgga
      601 gaccaaggac ctgatcgccg gctggatggt aattgatgtc gagacgcagg agcgggcgat
      661 cgagctggcc ggcgagcggt cggcagcgcc gggcgccggt gggaagccga tccacgagtg
      721 gctcgaagtg cgcccgttcc tgaccgggtc atcaaccacg acggagtgac agatggtgga
      781 tgaggctctg ctgcggaccc tcaccccaac cgtgatcggc atcctcgttc gtcgcggagc
      841 tcccttcgcg gcggccgagg atgccgtcca ggacgcgttg atcgaggccg tccgggtctg
      901 gccagctaac ccgccgcagg acgccaggag ctggctggtg accgttgcct ggcggaagtt
      961 cctcgacgcc acccgctccg agacgtcgcg acggcagcgc gaagacatcg tctcccgtga
     1021 gccgctgccc gaaccgggtg aggatgtaga tgacacgctc cagctctact tcctctgcgc
     1081 gcacccgtcg ctgacaccgg cgtcggctgt cgcgctgacg ctgcgtgcgg tcggcggtct
     1141 gacgacgcgg cagatcgcgc aggcttacct cgtgccggag gcgacgatgg cccagcggat
     1201 cagccgggcc aagcggaccg tctccggcgt tcgcttcaac cagcccggtg acgtcgcgac
     1261 cgtgctgcgc gtgctctacc tcgtcttcaa cgaaggctac tccggcgatg tcgacctcgc
     1321 cgccgaggcg atcaggctca cgcgtcaact ggcggcccgg atcaaccatg aggaagtggc
     1381 cggcttgctc gcgctgatgc tgctccacca cgcacgacgc ccggctcgga ccggccccga
     1441 cggcagcctc atcccgcttg ccgagcagga ccgcagccgc tgggataccc gcctgatcgc
     1501 cgaaggtatc gatctgctcc aggcggccct cgcccgcgac cggctgggcg agttccaggc
     1561 ccaggccgcc atcgccgcgc tgcacgccga cgcccagaca gccgccgaga ccgactgggt
     1621 ccagatcgtc gagtggtatg acgaactggt gcgcctgacc gacagcccga tcgcccgcct
     1681 caaccgggcc gtcgccgtcg gcgaggccga cggcccacgg gccggcctgg ctgccctagc
     1741 ggaactcgac ccatccctgc cccgttacgt cgccgtcgcc gcctacctgc acgagcgcga
     1801 tggcgacacc ccgacggcgg cacgcctcta cgccgacgct gcccggctgg caaccagcat
     1861 ccccgaacgc gaccacctca cgcgccaggc ctcccgcctc aacgcagcgt tgcgcgcctg
     1921 agctggtacc gggctacaga tctcgacgcg agaaggtcag gatcgctgcg ccgagcagga
     1981 cgactggata gagcagcgcc cagatgacca tcggcgtggc tggcgggacg ttgccgacga
     2041 agggcatccc ctcttcggcc gactggacgc cggccaggta gagcggcgag aggacgtagt
     2101 aggaggcgcc gcgccagatc gcgtcgctgg ggatcagcag gctgacggcc gtgccgatgt
     2161 tgagcatcga ctcgttggag agcgcgccgc cgatgaactc gatgatcccc gccagccagg
     2221 ccagcccgaa caggctgaag acgacgacac cgttggcgag cgtcgagagg atcgtgctgc
     2281 cgagcaggct gatggtcagc aggacgatcg ctgccagcat catcagcagc gtcgcgcgga
     2341 ccgggtcggg cgtcgtgtag tcggcgatca gccaggccga gagcagcagc gagccggcca
     2401 tcacgccgac gtagacgctg atcagtccgg cgtaggccag ccagcggccg gcgacgaact
     2461 cgactcgccg cagcggccgg gccaggaccg cgtgcagcgc tccggaatcg atctcgctgg
     2521 agatcgcgcc gaccgagagg aagagggcca ggaacccggc caggaagttg atcgcataca
     2581 gccccagcac cgacatgacc gtcccaaaca tcgcctgcgc ctgcgcatcg ctcaacgggc
     2641 cggagagttc gctgtcgttc tggtagatca ggaagaagcc cagcgcgaac agccccagga
     2701 aggccatgct gaggatcgtc ccggccagga tcagccgccg gctgatcgcc tcctggagcg
     2761 tgaagcgcgc gatcgtcagc gtgttcatga ctcgccacct tcgaccaggt ccatgaagat
     2821 ctcctccagc gttcggtgga ccggcaccag cccgaagagc tgatagccgc gccgcatgac
     2881 cgtctcggcg accagtggcg cgatctccag atcggagacc gccagcgtca gcgtcgcgcc
     2941 gtggctggtg gccggcgtcc cgtgctggtc atcggcgcca atctcttcgg cctccacgac
     3001 atcggcaacg gtcttcaacg cggccagcag ctcgctatcg actcgatcga ccatcacgcg
     3061 cagttcggag acgcccccgc tccggacgac ccggccgtgg tcgatgatcg cgacccggtc
     3121 gcagaccatc tcgacctcgc tgagcagatg cgagttgagg aagaccgtca cgccgtcgtc
     3181 gcgcagcgag cggatcaggt cgcgcacctc gcgccgcccg accgggtcga gcgccgaggt
     3241 cggctcgtcg agcaggacga gatcgggtcg gtggaggatg gcctgcgcca gcccgatccg
     3301 ctgggtcatg cccttcgagt aggtgcggat cttcgcgtcg cccttcccgg ccagcccgac
     3361 ccgcccgatc acctcgggaa tccggatccg ccgctcgtcg ggcgagagac cggccagccg
     3421 gccgtgaaag tcgagcagcc cggcgccggt cagccagtcg tggaagcgga agttctccgg
     3481 caggtagccg acccgccgcc gcgccgcgcg gtcgccagcc ggcagcccga agatacgcgc
     3541 ctcgccggcc gtgggccgga ccagccccat caggatcttg accgtcgtcg tcttaccggc
     3601 gccgttcggg ccgaggaagc cgaagatctc gccgggcgcc accgacagcg ccagatcgtg
     3661 caggactgcc gtccggccgt agaccttgcg caggcctgtc gtctcgattg ccgggatggc
     3721 agcggcggca gccatcctgg cgatcggctc agtggagaga tgcagcgaca cgcttcaact
     3781 cccgtgcctt catcgcgccc gcgacggcgt agatccgccc atcgcgctcc cagatcgcgg
     3841 cgctgccgag gccggtgttg tcgttgagca agaggcccgg gctaccggcg atcgtggccc
     3901 gctcccagtt gacctgatcg accgggatcg gcaccggcag cgtcgtcttc cagctgtcga
     3961 tctcctgcaa ctgctcaacc gtctccggcg agagcccagg cagccccagc aggaacgccc
     4021 gcaactcgtc cagcgtcacg ccgccctcgc tgccggcggt caccgttccg gcctgcccga
     4081 ccagcaggcc gataccgaac ggcgaactcg tgggagcagc cgcgttgcga tactcgagca
     4141 cgacagccgg cggcgtgttg atgatcagcg aggccccgtc gaaccgttcc gacagcgtga
     4201 catcgttctg cccgatcgac tggtagtagg cgcgggcctg gtcgaggtcg accgtgaacc
     4261 ggacctgatg cgccggcacg acccgcacct gcgccggcga ggggccgacc ccctccggca
     4321 gcgtcgccgg atccggctcc aggaccggaa acccgacggc ctggctcgcc gcggccacgc
     4381 ccgcaacgac ctgtgactcc ggggcggtct cgatgccgct gaccgtgccg aggtgctgca
     4441 tctcggacat cgtctgctcg acgttggcga tctggtcggt gctcagcgac acagccgcga
     4501 agcgttcgcc ccggaactcg gccaggaagt cagatgctgc tccgcgtccc gctgtcgtcg
     4561 ccagtatcag tatcagtacc agcgcaaccg cgagcccgct catgacgatt cgccagcgtt
     4621 ggatcggtaa cgcacctgtc atcgaacggt tctcccgaat gtcgcccatc ccaccgttac
     4681 gtagcagcat tatacatggc ggtactatgt atacaagata cgtgtcggga tggctgatcc
     4741 aatatcctca atgcccccaa tatcccaatc cggtaggggc gatttcgcaa aatcgcccct
     4801 accggattgg gatattggat tgtgttcgtt gcggattcaa tccgaagaca aagaa
//
LOCUS       DASUGJ010000581         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2040056, whole genome shotgun sequence.
ACCESSION   DASUGJ010000581 DASUGJ010000000
VERSION     DASUGJ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040056"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..>2492
                     /gene="dnaE"
                     /locus_tag="VFV93_13200"
     CDS             236..>2492
                     /gene="dnaE"
                     /locus_tag="VFV93_13200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX5166351.1"
                     /translation="MSSFVHLHLHTEYSLLDGLGRIDDYMTKAREYEMRHIAITDHGV
                     MYGVLDWYKAAKANDISPVVGVEAYLAPDGIAARNRRNYHLLLLAENERGYRNLLKLS
                     SRASIEGYYYRPRVDLEMLNELSDGIICTSACLGGPVANPYLDGRDEEAEQLAGTLRE
                     MFGPERFYLEVQDHGMAEQQRVNRQLIDLARRMDLPLVASNDVHYVNREDAPIQDLLV
                     CIQTNTTLDDPKRLKMESDQLYFKSPREMEAIFGDLPEALENTVRIAERCAFDLDFSR
                     LHLPDPGIPEGTTAQEYLSELCWKGLAERYPNVTDEVRQRLEYELYVIEQTGFGIYML
                     IVRDFALFARENEIPFGVRGSAAASIVLYTLGITDIDPIANRLVFERFLNLERREMPD
                     IDMDFADNRRAEVIDYVARKYGHDRVAQIITFGTMGAKASVRDVGRALAWTPSDVDRI
                     ARLIPSALNMTLERALTESSELRSLYDTDERSRTLIDNARRLEGISRHAGTHAAGVVI
                     SADPLVDHVALQRPARGDEGALPTTQFTMERVAEIGLLKMDFLGLANLTILGEAVAII
                     KQTRGESLDPKLFPDGDADTYAMLAKGETFGVFQLESTGMRRAIQELKPETVSELSAM
                     VALYRPGPMQHIPTFCRAKHGQEEIRYPHPDLADVLDETYGVIVYQDQVLLIARQFAG
                     YTLGEADIMRKAMGKKIAEKMRAERERFNAGAVEKGYSADDADKIFDLIEPFAGDAFN
                     KAHAVCYGTIAY"
BASE COUNT          465 a          796 c          766 g          465 t
ORIGIN      
        1 acagagagcc gcaatcgcac ccggccgtgt cgcccaccac gattcgatcg ccgccgcctg
       61 attcctctga attgcacact ggacggcggc tggcactcgc cagcggccgt ccggtgtctt
      121 atccttagcg cggcgctcgt acgtacgacc cggttgtgcg tacgtacgct gagatctcag
      181 gcgcttgttt caccgccctg gccacgccgg gtcgtacccc gtactggagg agtccatgtc
      241 gtcgttcgtc cacctgcacc tacacaccga gtattcgttg ctggatgggc tgggacgcat
      301 cgacgactac atgaccaagg cgcgcgagta tgagatgcgc cacatcgcga tcaccgatca
      361 cggcgtgatg tacggcgttc tcgactggta caaggccgcc aaagcgaacg acatctcgcc
      421 cgtggttggc gtcgaggcct atctcgcacc ggacgggata gccgcccgta accgtcgcaa
      481 ctaccacctg ctgctgctgg ccgagaacga gcgcggctac cgcaatctgc tcaagctctc
      541 cagccgggcc agcatcgagg gctactacta ccggccccgc gttgacctgg agatgctcaa
      601 cgagttgagt gacgggatca tctgtacctc agcctgcctc ggcggcccgg tcgccaaccc
      661 gtacctggac ggccgtgacg aagaggcgga acagctggcc gggacactac gcgagatgtt
      721 cggacccgag cgcttctatc ttgaggttca ggatcacggc atggccgagc agcagcgcgt
      781 caatcggcag ttgatcgacc tggcgcgacg gatggacctg ccgctggtgg cctccaacga
      841 tgtgcactac gtcaatcgcg aggatgcgcc gattcaggat ctgctggtct gcatccagac
      901 caacacgacg ctggacgatc cgaagcgcct caagatggaa tccgaccagc tctatttcaa
      961 gtcgcctcgc gagatggaag cgatcttcgg tgatctaccc gaggcgttgg agaacaccgt
     1021 ccggattgcc gagcgttgcg cttttgatct cgacttcagc cggctccact tgcccgaccc
     1081 cggcattcca gagggcacga cggcgcagga gtatttgtcc gagctctgct ggaaggggtt
     1141 ggccgagcgc tatccgaacg tgaccgacga ggtgcgccag cggcttgagt atgagttgta
     1201 cgtcattgag cagaccggtt tcggcatcta catgctgatc gtgcgtgact tcgccctgtt
     1261 tgcgcgcgag aacgagattc cgtttggcgt ccgcggctcg gcggctgcca gcatcgtcct
     1321 ctacacgctg gggatcaccg acatcgatcc gatcgccaac cggctcgtct tcgagcgctt
     1381 cctcaacctc gaacgccgcg agatgcccga catcgacatg gacttcgccg acaaccgccg
     1441 cgcggaagtg atcgactacg tggcccgtaa gtacggtcac gaccgggtgg cgcagattat
     1501 tacgtttgga acgatgggtg ccaaagcctc ggttcgcgac gttgggcggg cactggcctg
     1561 gacgccgagc gatgtcgacc ggatcgctcg cctgatcccg tccgccctca acatgacact
     1621 ggagcgtgcg ctgaccgaga gcagcgaatt gcgctcgctc tacgacacgg atgagcgctc
     1681 gcgcacgttg atcgacaacg ctcgccgcct tgagggcatc tcccgacatg ccggaacgca
     1741 tgccgccggc gtcgtcatca gcgccgaccc gctggtcgac cacgttgcgt tgcagcgtcc
     1801 ggctcgcggg gacgagggtg cgctaccgac gacgcagttc accatggagc gtgtcgccga
     1861 gattggcctg ctcaaaatgg acttcctcgg ccttgccaac ctgacgatcc tcggcgaggc
     1921 cgtcgcaatc atcaagcaga cgcgtggtga atcgcttgat ccaaagctct tccctgatgg
     1981 cgacgccgat acgtatgcga tgctggccaa gggcgagacg ttcggcgtct tccagttgga
     2041 gagtaccggc atgcgtcgcg ccattcagga actgaaaccc gagaccgtct cggagctgtc
     2101 cgcgatggtc gcgctctacc ggccgggccc gatgcagcac atcccgacct tctgccgcgc
     2161 caagcacggt caggaggaga tccgctaccc gcaccccgat ctggcggatg tgcttgacga
     2221 gacgtacggt gtcatcgtct accaggatca ggtgctgttg atcgcgcgtc agtttgccgg
     2281 ctacacgctc ggcgaggccg atatcatgcg caaggcgatg ggaaagaaga tcgccgagaa
     2341 gatgcgtgcc gagcgggagc gtttcaacgc tggcgctgtc gagaagggtt attccgctga
     2401 cgatgcagac aagatcttcg acctgatcga gccgttcgcc ggcgatgcct tcaacaaggc
     2461 gcacgccgtc tgctacggga cgatcgccta tc
//
LOCUS       DASUGJ010000582         4923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2040721, whole genome shotgun sequence.
ACCESSION   DASUGJ010000582 DASUGJ010000000
VERSION     DASUGJ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040721"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2108)
                     /locus_tag="VFV93_13205"
     CDS             complement(<1..2108)
                     /locus_tag="VFV93_13205"
                     /inference="COORDINATES: protein motif:HMM:NF015387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEX5166352.1"
                     /translation="MSVGFYAQFGGSQPAEAAPGPVALPGTFQLEDYTSFYDTTRGNS
                     GRTYRNDDVDIQRCYDLALRAYCYNVGWIAPGEWLGFNVTVAKSGTFTFSTRVASIAR
                     TGAFHIEVDGVNVTGKQAVPFTRSTMLWTDVVSQPVAIAAGEHNVQIVAEGSNFSLDS
                     MTVTQTSVDAPAQPTPAPTQEPTATPTQVPTQAPTATPTQPTATPVASSPQIPATVAL
                     PGTFEAEHYTSFSDTTPGNLGKRYRTDDVDIEDCTDPESAAKCYDVAWIKSDEWLGYD
                     MTVATTGSFTFTTRVASPNDGRSLRFEVDGVDITGEIAVPNTGGYQNWTSVSSNPVSL
                     TAGNHTLKMVFTGSSFNVNNVTVSEVKTEAPVQTEPVQTEPENGNAHALPGTFEVEDY
                     SSFSDATAGNYGKRYRNDDVDIEDCSDAAPAGQCYDVGYVDAGEWLGYDVNVAKNGTF
                     TFSTRVATSKNNRHFRFEVDGADVSGQIAIPNTGGYQSWQTVTSSPVSLTAGKHTLRI
                     VAGESNFNLNNVTVAEVADASPAPAPSTDPAPAPAPEVNGPTYYVSPTGNDTADGSLA
                     NPWRNLSSSMKKLRAGDTLLVRGGTYRERVEVRGESVPRGTADARITVKAYPGEEPLV
                     VGIFWISNPDYWTFDNIDVTYDPATNNTQEHMVRMYRGHDWIFQNSEIYGAHSYAGLL
                     VNGKSKNWLITNNYIHDTAPS"
     gene            complement(2738..3643)
                     /locus_tag="VFV93_13210"
     CDS             complement(2738..3643)
                     /locus_tag="VFV93_13210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5166353.1"
                     /translation="MPMANHSDDADRSSKATNDMTRYDRVARMDMARGDASVRDNRQM
                     TQLVDVDEHVERERQRYNAQFQPYRQGHLRVKYGAEFAPSDQRAALLYALDQLGDLRG
                     KRVLEIGGGTGYHAAILVHRGAQVVATDVSSVGVEVMQRRFEANEMTDRAEAAVVVAE
                     EMPFEDGSFDAVFGVAVLHHLVMDSAGPEIARVLRKGGRAVFLEPLSENPVLDFVRDR
                     VPYPGKKSPHGHRGVTFAMLEQASHSFASIRIRPFYLTSMLNRALGQSTSITPLEKLD
                     DMLIARSTRTHRLCRQVVVTFATAP"
     gene            3730..4089
                     /locus_tag="VFV93_13215"
     CDS             3730..4089
                     /locus_tag="VFV93_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166354.1"
                     /translation="MGRDYTQYERNERRNFGGPDDWESDIEEGIDQRERDRSTSRSAW
                     QGSPVTSYREADPRGRDDWERYDEGSRQRSVYWGRRPNEARNQPSHGYELDHDVYRAG
                     DRDEWHGEFGPKEDRIQ"
     gene            4117..4857
                     /locus_tag="VFV93_13220"
     CDS             4117..4857
                     /locus_tag="VFV93_13220"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5166355.1"
                     /translation="MRGAVREGRDWLASGRDRYRDWRETGGDPYRERRAQYEGTRYSR
                     DSGWVRPGSYAGRGPRGYQRTDARIREDVSEILWRADEIDASDIDVTVENGEVTLDGS
                     VDTRWTKRLAEDAAWEASGVYDVHNRLRIRRVDRDPIATGMRDTSGAIPMESGRNTTS
                     PRFLVNMAVVGNNGDEVGIIKEVREDTLLVDRPMSRDIYIPVSAVKISDGSTARLNID
                     SGEVDNQGWQHPPIAGTDTDYDERVDLT"
BASE COUNT          880 a         1518 c         1683 g          842 t
ORIGIN      
        1 gacggagccg tatcgtggat gtagttgttc gtgatcagcc agttcttcga cttaccgttg
       61 acgagcaggc cggcgtagga gtgcgcgccg tagatctcgc tgttctggaa gatccagtcg
      121 tgcccgcggt acatgcggac catgtgctcc tgcgtgttgt tggtggctgg gtcgtaggtc
      181 acgtcgatgt tgtcgaaggt ccagtagtca gggttcgaga tccagaagat accgacgacc
      241 agtggctcct cgcccgggta ggccttgacg gtgatgcggg catcggccgt gccacgcggg
      301 accgactcgc cccgaacctc gactcgctcg cggtaggtgc cgccacggac cagcagcgtg
      361 tcgccggcgc gcagcttctt catcgaggag gacaggttgc gccacgggtt ggccaggctg
      421 ccgtcggcgg tgtcgttgcc ggtcggcgag acgtagtagg tcgggccgtt gacctccggg
      481 gccggcgccg gtgccggatc ggtggacgga gccggcgcgg gagacgcatc cgcgacttcg
      541 gcgacggtga cgttgttcag gttgaagttc gactcgccgg cgacgatccg cagcgtatgc
      601 ttgccggccg tcagcgagac cgggctgctg gtcaccgtct gccaggactg gtagcctccg
      661 gtgttcggga tcgcgatctg gccggagaca tcagcgccgt cgacctcgaa gcggaagtgg
      721 cggttgttct tcgacgtcgc aacgcgggtc gagaaggtaa acgtaccgtt cttcgcgacg
      781 ttcacatcgt agccaagcca ctcaccggcg tcgacatagc cgacatcgta gcactggccg
      841 gccggcgcag cgtcgctgca atcctcgatg tcaacgtcgt cgttgcggta acgcttgccg
      901 tagttgccgg cagtcgcgtc ggagaagctg ctgtagtcct cgacctcgaa ggtgcccggc
      961 agcgcgtggg cgttcccatt ctccggctcg gtctggaccg gctcggtctg gaccggtgcc
     1021 tcggtcttga cctcgctgac ggtcacgttg ttgacgttga agctcgaccc ggtgaagacc
     1081 atcttcagtg tgtggttgcc ggccgtcagt gagaccgggt tgctgctgac gctggtccag
     1141 ttctggtagc cgccggtgtt cgggacggcg atctcgccgg tgatatcaac gccatcaacc
     1201 tcgaagcgca ggctgcggcc atcgttcggc gaggcgaccc gcgtcgtgaa ggtgaacgag
     1261 ccggtcgtcg cgacggtcat gtcgtagccg agccactcgt cggacttgat ccaggccacg
     1321 tcgtagcact tggcggccga ttccggatcg gtgcagtcct cgatatcgac atcatcggtg
     1381 cggtagcgct tgccgaggtt gcccggcgtc gtgtcggaga agctggtgta gtgctcggcc
     1441 tcgaacgtgc ccggcagcgc cacggtcgcc ggaatctgcg ggctgctcgc gaccggcgtc
     1501 gcggtcggct gggtcggcgt cgccgtcggg gcctgagtcg gcacctgggt cggcgtcgcc
     1561 gttggctcct gcgtcggggc cggcgtcggc tgggccggcg catcgactga ggtctgggta
     1621 accgtcatcg agtcgaggct gaagttcgac ccctcggcaa cgatctggac gttgtgctcg
     1681 ccggccgcga tcgcaaccgg ctggctgacg acatcggtcc agagcatcgt gctgcgggtg
     1741 aacggaaccg cctgcttgcc agtgacgttg acgccatcga cttcaatatg gaaggcgccg
     1801 gttcgggcga tcgaggccac gcgggtcgag aaggtgaacg tgccgctctt ggcgaccgtc
     1861 acgttgaagc cgagccactc gccgggcgcg atccagccga cgttatagca gtaggcgcgc
     1921 agcgccagat cgtagcagcg ctgaatatca acatcatcat tgcgataggt gcgaccgctg
     1981 ttaccacggg tcgtgtcgta gaagctcgta tagtcctcaa gctggaaggt acccggcagg
     2041 gcaaccggcc cgggggcggc ttctgcgggc tgcgaaccgc caaactgagc gtagaaacca
     2101 acagacaacg ccacaaccac aatcaaggtc aatgtcgcat gcccaaatcg acgcaaccgt
     2161 gtttgctgat tcataaaacc agacacatcc tcactaccga cactcgcgac caaacaaatg
     2221 ggacattccc catgtcggac atccgccgca ggacgagcga tcaggcgact caactggccg
     2281 ttgactgcct ggcaccgcaa aaggcgatgg ccgtcccgcg tctgttgtga gggtagggag
     2341 cgaggggccg ctggcgtatg cgttgatcgt ccggaagcac cgcccccact tcgggctagc
     2401 ccgagcggga caaacctcct acgcacatcg ggaaggtcgc gaacgtacgc gggaagagtg
     2461 gcgtgttggg tgcatgctgg gtccgcattc agacggcgac gaggcggtgt tatgtcgttg
     2521 caatcggtcg ggttcagcgg gcagaacggg attgcgagcg cgcacggcga aaccggcgcc
     2581 aacggcatgt cccaccgccg ccaacggtgt tccggtggtc ccgacttttc tcgttagtgc
     2641 gggatgtatt acttgcgcaa caccgtgaca ggcaggcagc acgaagcggc agttgtggtg
     2701 gtatgtgacg gtggtggcaa cgcgaaggtg cgtcgcgcta cggagcggtc gcgaacgtta
     2761 cgaccacctg gcggcagagg cggtgggtcc gtgtcgatcg cgcgatcagc atgtcatcca
     2821 gcttttccag cggggtgatc gatgtcgatt ggccgagcgc acggttcagc atcgacgtca
     2881 ggtagaacgg ccggatccgg attgaggcga acgagtggct ggcctgctca agcatcgcga
     2941 aggtgacgcc gcggtggccg tgtgggctct tcttgccggg gtaggggacg cggtcgcgga
     3001 caaagtcgag gacgggattc tcgctcagcg gttccaggaa gacggcgcgt ccacccttgc
     3061 ggagcacgcg agcgatttct ggcccggcgc tgtccatgac caggtggtgg agaaccgcga
     3121 cgccgaagac ggcgtcaaat gacccatcct cgaacggcat ctcttcagca acgacgacgg
     3181 cggcttcggc gcggtcagtc atctcattcg cctcgaagcg gcgctgcatg acctcaacac
     3241 cgaccgacga cacatcggtt gcgacaacct gcgcgccgcg atggacgaga atcgcggcgt
     3301 ggtagccggt gccgccacca atctccagta cgcgcttgcc gcgcagatcg ccaagctggt
     3361 cgagcgcgta gaggagggcc gcgcgctgat cgctgggggc aaattccgcc ccatacttca
     3421 cccgcagatg gccctggcga tagggctgga actgcgcgtt gtagcgctgc cgttcgcgct
     3481 cgacatgctc atccacgtcc acgagttgtg tcatctgccg gttgtccctc acgctggcgt
     3541 cccctctggc catgtccatc cgtgcgacac gatcgtaccg cgtcatgtca ttcgtcgctt
     3601 tggatgatcg atcagcatca tcggaatgat tcgccatcgg catgcaactt gcacccgccg
     3661 ggatgacagc cggacaggcc gtcggacggg cagagcagac acgatcgagc agcggaaagg
     3721 agtccgcgcg tgggacggga ctacacgcag tacgaaagga acgagcgacg caacttcggc
     3781 ggtccggacg actgggagag cgacatcgag gaagggatcg atcagcgcga acgcgaccga
     3841 tcgaccagca ggtccgcctg gcagggcagc ccggtgacca gctatcgcga ggccgatccg
     3901 cgcggccgtg atgactggga gaggtacgac gaaggcagcc gccagcgatc cgtctactgg
     3961 ggccgccggc cgaatgaggc acgcaaccag cccagccacg gctatgaact ggaccacgat
     4021 gtctaccgcg ccggcgaccg cgatgagtgg catggcgagt tcggccccaa ggaagaccgc
     4081 atccagtgag gtcgcgaccg gcgctggcgg aagacgatgc gcggcgccgt ccgtgaggga
     4141 cgcgactggc tggcatccgg ccgcgaccgc taccgcgact ggcgcgagac tggcggcgat
     4201 ccctaccgcg agcggcgtgc tcagtacgaa gggacgcgct acagtcgcga cagcggctgg
     4261 gtccgccctg gcagctacgc gggccgcggg ccacgcggct accagcgcac cgatgcacgc
     4321 attcgcgagg atgtcagcga gatcctctgg cgggctgacg agatcgacgc cagcgacatt
     4381 gatgtcacgg ttgagaacgg agaagtgacg ctcgacggct cggtcgatac gcgctggacg
     4441 aagcggctgg ccgaggatgc cgcctgggaa gccagcggcg tctacgatgt ccacaaccgg
     4501 ctgcggatcc ggcgggtgga tcgcgacccg atcgcgaccg gtatgcgcga caccagcggc
     4561 gcgattccaa tggagagtgg ccggaacacg acctcgcccc gcttcctggt caacatggcc
     4621 gtcgtcggca acaacggcga cgaagtcgga atcatcaagg aagtgcgcga agacacgttg
     4681 ctggtcgacc ggccgatgtc acgcgatatc tacatccccg tcagtgccgt caagatcagc
     4741 gacggcagca cggcccgcct caacatcgac agcggcgaag tcgacaacca gggctggcag
     4801 catccgccga ttgccggcac cgacaccgac tacgacgagc gcgtcgatct gacctgatgc
     4861 gtcctgcacg ctccgtcccc cgcgtagggg cgatggtcgc agcctcgccc ttcgtcgtgc
     4921 gat
//
LOCUS       DASUGJ010000583         4674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1901264, whole genome shotgun sequence.
ACCESSION   DASUGJ010000583 DASUGJ010000000
VERSION     DASUGJ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901264"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VFV93_13225"
     CDS             complement(<1..351)
                     /locus_tag="VFV93_13225"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX5166356.1"
                     /translation="MNRLRLLIALLIGIVVVAAAAWYLTGRGEDQQTAVAERGSLDVT
                     IQTVGTLQATGATTVRSIQSGIVETVGVRAGDVVEEGDIIALLEMEPFDRNVQQAQRE
                     FDQAEFALQAAEQQA"
     gene            complement(344..2686)
                     /locus_tag="VFV93_13230"
     CDS             complement(344..2686)
                     /locus_tag="VFV93_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5166357.1"
                     /translation="MRTLVSKVLRDLRLRPLRNLLTILGIVLGVAGVVAISATARSIA
                     DAQRLTYANSRQADLATFANGMSATTANLVERQPNVVAAETRSVTFTRFDTGTGWQNL
                     RLVGLESFDQMQLDLVEVVEGRVPQRGEVAFDESSRELAPIELGTVVAIRESPADPLV
                     YVTVVGFTRSPATLGAGLMNRATAYVPAATVRELTGMTGDNYLLVRVEDQQRASQTAA
                     GISRLLSKRGVSLSSFDVRDPDAFVGSRELGTLLLLLRVFSWLGAALSSVLVANTLAA
                     VMGEEMTQIGIIKSVGGQRRQIILTYLLYGSILGVIGTVTGWLAGLAIGQQISGYLTR
                     LTGLQQPAFTITAREIGLALLVGTLVTLTASLLPVIANANARVASLLRTQGVRNERTS
                     QTLLRVSAPLAHLSVAAAVGMRNALRRPRRTLSTILVVTVAVAAFVATQALSRSVSGT
                     VDELYALYGADGWVSYERPVGLDFASVLAEDPWITEVEPWTSASGAIGSTRTDIWGMP
                     ADDPLYSYRLLEGTWVRQSNPPSAVLTSNLAAAIGARVGDWRELDVGQKRETVQIVGI
                     VNDSSTYLGNAATGKLFMRVEDVNRLHSLGQQADVFAYKLTASDPDSVDTALAEIEER
                     TRAYGPVTYSSYSDQQSSRQAIGILTLMLNAMVVVVAVVGVSGIANTLLISIAERRRE
                     FGVMRAIGAGTRQIVTVLVSEGLMLALLGLIIGTIAGYPLARLLVDITSAELFDLTFY
                     LSPASIVMTFVVALLTVAAVSALPGLVAARIRPIQVLRYE"
     gene            complement(2695..3420)
                     /locus_tag="VFV93_13235"
     CDS             complement(2695..3420)
                     /locus_tag="VFV93_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166358.1"
                     /translation="MDDRQPAICVEDVYRRYRTGRRAIPALAGVSLSVEYGEWIAIVG
                     PSGCGKSTLLNLLAGIDSADDGSVAVAGQTLNGMSENALAAWRGREVGIVFQFFQLMP
                     TLTVLENVILPMDLAGNTTDRQTRAMQLLEQVGVADLAGNLPSELSGGEQQRVAIARA
                     LANAPRLLLADEPTGNLDSRNGQIVVDLLDELWRQGTTIVMVTHDPEVASRASRIISM
                     RDGQIVDEGDGQPLPLVALAANG"
     gene            complement(3422..3679)
                     /locus_tag="VFV93_13240"
     CDS             complement(3422..3679)
                     /locus_tag="VFV93_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5166359.1"
                     /translation="MDLDPGGIISWLIVGLLAGWIAGIVTRGGGFGCVTNIVIGVIGA
                     FIGGLILEVLNVNGTAGFLESLAIATLGAVILLAIANLARR"
     gene            complement(3679..4590)
                     /locus_tag="VFV93_13245"
     CDS             complement(3679..4590)
                     /locus_tag="VFV93_13245"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5166360.1"
                     /translation="MFRGIFPPTVTLFKEDGSLDPEANQCHHDFLIESGVQGLFILAT
                     TGEFMHMNLKDREQHAAETMQHIAGRTLVIVGTGTTSTRETIRLSRHAQGVGADAVAI
                     ITPYFWTLSERELIGHLSAVANAVDIPIFVYNFPAYSNFNIGSETLVSLVREHRNIAG
                     VMDAIDSLEHLRQRVQLVKAVNPEFRVLAGSDGHLLNILQLGGDGTVPATANIAPDRH
                     VKVFNAYERRDYQRALELMPAVLSCLDMFRITGSFHSVVKEAMVMAGIATSATPRQPA
                     LPLTAHSRARLREVLARANLLPAPEET"
BASE COUNT          827 a         1520 c         1540 g          787 t
ORIGIN      
        1 ggcttgttgt tctgctgcct ggagcgcgaa ctcggcctga tcgaattccc gttgggcttg
       61 ctggacgttg cgatcgaacg gctccatctc gagcagcgcg atgatgtctc cctcttcgac
      121 cacgtcgccg gcccggaccc cgaccgtctc gacgatgcct gactggatcg agcgaacggt
      181 cgtcgcgccg gtcgcctgga gcgtgccgac cgtctggatc gtgacgtcga gcgacccgcg
      241 ttcggcaacg gccgtctgct ggtcctctcc acggccggtc aggtaccagg cagcggcagc
      301 gacgacgacg atgccgatca gcagggcgat cagcagccgg agcctattca tagcgcaaca
      361 cctggatcgg cctgatccgg gccgcgacca gtcccggcag tgctgagaca gccgcgaccg
      421 ttaggagcgc gacgacgaac gtcatcacga tcgaggccgg agagagataa aacgtcaagt
      481 cgaacagctc tgcgctggtg atgtctacca gcaaacgtgc cagtggatat cctgcaatag
      541 tcccaatgat cagccccagc agtgccagca tcagcccctc gctgacgagg accgtcacga
      601 tctggcgcgt cccagccccg atcgcgcgca tgacgccgaa ctcccgccgt cgctcggcga
      661 tgctgatcaa cagcgtgttg gcgatcccag agacgccgac gacggcgacg acgacaacca
      721 tcgcgttcag catcagtgtc agaatgccga tcgcctgccg cgagctctgc tggtcggagt
      781 acgacgagta ggtgacgggt ccgtaggcgc gggtacgttc ctcgatctct gccagtgctg
      841 tatcgacgct gtcgggatcg gacgccgtca gcttgtaggc gaagacatcg gcctgctggc
      901 ccagcgagtg gagccggttg acatcttcga cgcgcatgaa cagcttgccg gtagccgcgt
      961 tgcccaggta ggtgctggag tcgttgacga tgccgacgat ctggaccgtc tcacgcttct
     1021 ggccgacatc gagctcgcgc cagtcaccga cgcgcgcgcc gatcgcggcc gccaggttgc
     1081 tcgtcaggac ggcgctgggt gggttggact ggcgaaccca ggtcccttcg agcagccgat
     1141 agctgtagag tggatcgtcg gccggcatgc cccagatatc ggttcgcgtc gagccgattg
     1201 cgcctgacgc gctcgtccac ggctcaacct cggtgatcca cgggtcctcg gccaggacgg
     1261 acgcgaagtc gagtccaacc ggacgctcgt acgagaccca gccgtctgcc ccgtagagtg
     1321 cgtacagctc atcgaccgtg cctgagaccg accggctcag cgcctgggtg gcgacgaatg
     1381 cggcgaccgc gaccgtcacg accaggatcg tcgagagtgt ccggcgcggg cgacgcagtg
     1441 cattgcgcat gccgaccgcc gctgcgacgc tcaggtgtgc tagtggcgct gacactcgca
     1501 gcaatgtctg gctggtgcgt tcgttgcgaa cgccctgtgt tcgcagcagc gaggctacgc
     1561 gcgcgttggc gttggcgatg accggcaaca gcgacgcggt gagcgtcacc agtgtgccga
     1621 ccagcagcgc cagcccgatt tcgcgggcgg tgatcgtgaa ggccggttgt tgcaggccgg
     1681 tgaggcgtgt gagatagccg ctgatctgct ggccgatcgc cagcccggcc agccagccgg
     1741 tcacagtgcc gatcacgccg aggatcgaac cgtacaagag gtaggtcagg atgatctggc
     1801 ggcgctggcc gccgacgctc ttgatgatgc cgatctgcgt catctcctcg cccatgacgg
     1861 ccgccagcgt gttggcaacc agaacagacg agagcgccgc cccaagccac gagaacaccc
     1921 gcagcagcag cagcaacgtc cccagctcgc gcgagccgac gaaggcgtcg gggtcgcgaa
     1981 catcgaagct ggagagcgag actccccgct tcgataacaa cctgctgatg ccggcggccg
     2041 tctggctggc gcgttgctgg tcttcaaccc gtaccagcag gtagttgtcg ccggtcatgc
     2101 cggtcagctc gcggacggtg gcggccggca cgtaggccgt cgcccggttc atcagtcccg
     2161 cgccgagcgt tgccggcgaa cgggtgaagc cgacgacggt cacgtagacg agcgggtcgg
     2221 caggcgactc acggatggcg acgacggtgc cgagctcgat cggcgccagc tcccggctac
     2281 tctcatcgaa ggcgacctca ccccgttgcg ggacgcgtcc ctcaacgacc tcgaccagat
     2341 cgagctgcat ctggtcgaac gactccagcc cgaccagccg caggttctgc cagccggttc
     2401 ccgtgtcgaa gcgcgtgaac gtcaccgagc gtgtctcggc cgcgacgacg ttcggctggc
     2461 gttcgaccag attggcggtt gtcgcgctca tgccattggc aaacgtcgcc agatcggcct
     2521 gccggctgtt ggcatacgtc agccgctggg catcggcaat cgagcgcgcg gtggctgaga
     2581 tggcgacgac gccggcgaca ccgaggacga tgccgagaat cgtcagcaga ttgcggagtg
     2641 gccggagacg cagatcgcga agaaccttgc tgacgagtgt tcgcatggcg atggtcagcc
     2701 gttcgcagcg agcgcgacaa gcggaagcgg ctggccgtcg ccctcgtcaa cgatctggcc
     2761 gtcgcgcatg gagatgatgc gcgaagcgcg cgaggcgact tccggatcgt gcgtcaccat
     2821 cacgatcgtc gtgccctgtc gccagagctc gtccagcaga tcgacgacaa tctggccgtt
     2881 acgcgagtcg agattgccgg tcggctcgtc agccagcagc agccttggcg cgttggcgag
     2941 cgcccgcgca atcgcgactc gttgctgctc gccgccggag agctccgacg gcaggttgcc
     3001 ggccaggtcg gcgacgccga cctgttcgag cagctgcatc gcccgcgtct gccgatcggt
     3061 tgtgtttccg gccagatcca tcggcaggat gacattctca aggaccgtca gcgtcggcat
     3121 cagttggaag aactggaaga cgatcccgac ctcccggcca cgccaggcag ccagcgcgtt
     3181 ctcgctcatc ccgttcagcg tctgtcccgc gacggcgaca gaaccgtcat cggcgctgtc
     3241 gatgccggcc agcaggttca gcaacgttga tttaccgcaa cctgaggggc cgacgatcgc
     3301 gatccactcg ccgtattcaa cgctcaacga gacaccggcc agcgccggaa tggcgcggcg
     3361 tccggtgcgg tagcgccggt agacgtcttc gacgcagatg gctggttgcc ggtcgtccat
     3421 gtcaccttcg ggcgaggttg gcgatcgcaa gcagaatgac ggcgccgagg gttgcgatcg
     3481 cgaggctttc caggaatccg gcggtcccgt tgacgttgag tacttcgagg atcagcccgc
     3541 cgatgaaggc gccgatgacg ccgatcacga tgttcgtgac gcagccgaat ccgcctcctc
     3601 gcgtaacgat accagcgatc cagccggcca gcagtcccac gatcagccag gagatgattc
     3661 ctcccggatc gaggtccact aggtctcctc cggcgccggc agcaggttgg cgcgggccag
     3721 cacctcacgc aaccgcgcgc gactgtgagc cgtcagcggc aaggccggct ggcgcggagt
     3781 cgcggaggtc gcgatcccgg ccatcaccat cgcttccttg acgacgctgt ggaacgaccc
     3841 ggtgatccgg aacatgtcca gacagctcag gacggccggc atcagttcca acgcgcgctg
     3901 gtagtcgcgc cgctcgtagg cgttgaagac cttgacgtgg cggtcgggcg cgatattggc
     3961 cgttgccggc accgtgccgt ccccgccgag ctggaggata ttgagcaggt gcccatcgct
     4021 gccggccagg acgcggaact cggggttgac cgccttgacg agttgcaccc gctggcgcag
     4081 gtgctccagg ctgtcgatcg cgtccatcac gccggcgatg ttgcggtgct cgcgaacgag
     4141 cgagacgagc gtttcgctgc cgatgttgaa gttcgaataa gcgggaaaat tgtagacgaa
     4201 gatcgggata tcgacggcgt tggcaaccgc cgacagatgc ccgatcagtt cgcgctccga
     4261 cagcgtccag aagtacggcg tgatgatcgc gaccgcgtcc gctccaaccc cctgggcatg
     4321 ccggctgagc cggatcgtct cgcgcgtcga ggtcgtgccg gtgccgacga tgaccagcgt
     4381 ccggccggcg atatgctgca tcgtctcagc cgcgtgctgt tcgcggtcct tgagattcat
     4441 gtgcatgaac tcgccggtcg tcgccaggat gaacagcccc tggaccccgg actcgatcag
     4501 gaagtcatga tgacactggt tcgcttcggg atcgagcgaa ccatcctcct tgaacagcgt
     4561 gaccgttggc gggaatatgc ctcgaaacat cgcaccccct ggcgtctaca acgagaccga
     4621 tggagtgtag cacgcggccc gaatctcaat tcggttggtc agacccggcc aaca
//
LOCUS       DASUGJ010000584         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_790493, whole genome shotgun sequence.
ACCESSION   DASUGJ010000584 DASUGJ010000000
VERSION     DASUGJ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790493"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VFV93_13250"
     CDS             <1..1064
                     /locus_tag="VFV93_13250"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX5166361.1"
                     /translation="PGNTISVALDMRLAGYRGGGIARYARELRAALGGASSIAVRGIR
                     SQRDTGGADGDLRVRTPPHHRLERFALPVEMALARYQPKVFHATDFIAPSMPRSAVVA
                     TVHDLAFIHWPNDLAPDALEYYRQLGDARRRTDAWITPSRWTADDLAGIYGIDRDTIH
                     VIPHGVSLDLMRADPLPRVGRGDYVLAVGTVEPRKRYDLLLDAIQERDDLPRLVIAGG
                     SGWNSQMLENRLRLAARAEWVADAGDDALRRLYREAIALVVPSRAEGFGLPALEAMAV
                     GTPVVSSGGGALPEVTGEAALAVAEASGEAWAAAVERIASDNALWEQLSAAGRVRARR
                     FSWDAAARATAEVYRNLVT"
     gene            1147..2148
                     /locus_tag="VFV93_13255"
     CDS             1147..2148
                     /locus_tag="VFV93_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5166362.1"
                     /translation="MSRAQGEPVSTSVLHVIINPAAGSGRAGRRWPELEAEMFRHGYE
                     VRPSFTTGPGDAIRLARELAANGARTIVCVGGDGTVNEVVNGLIDGGRPVNPETRLAV
                     IPCGTGKDLCRTIGTRDVTSMLRALADDTTALIDLGRVSCRDAKTGEPVERYFANVAD
                     TGIGAETAARINASSKALGGFVSYLSGAVRSIVAFKPWQVTVEVDGEPVHSGSAGMVV
                     FANGRYFAGGMNVAPDASLCDGKLDIFVLEGVGKRALLTSLLPRVYRGRHVGQPGVVH
                     LTGSQATVTCAAGMLLELDGEQIGTTPLAVEVLPAALRIVGTQDALAAAGGCTERAE"
     gene            2332..3468
                     /locus_tag="VFV93_13260"
     CDS             2332..3468
                     /locus_tag="VFV93_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5166363.1"
                     /translation="MSTAYTNDGLGEHLDEMPFTLHGKPASELQNWFWQGLKAEMHRQ
                     GHAYRPEIVDDIRLVVNFTRLDEPQPFRRKAQGTFVVTIVEDTSDPADIIRAGYPVLV
                     RALSNLGIYLVPVGDRVIAHFLTMEQGHYIVELAEDESEERFFGEVYERLAPLATSQL
                     VINNEYLPDLPAALWDGDEITRQLTWAGEQLGDLALLPAPWPIQEILSDRDLKHVMRL
                     FGIGGLSYGNLSARYDDQRFWMSASGVDKTNLREIGRDILMVSDYVRERNAMVLSVPP
                     HIQPRRVSVDAIEHFMIYREHPTVGAIIHIHAWMDGIDSTHINFPCGTRELAVAVSDL
                     VRNAPDPSRAVVGLKNHGMTLTGHSLPEIFERIEGKIIPQVPMS"
     gene            3469..>3795
                     /locus_tag="VFV93_13265"
     CDS             3469..>3795
                     /locus_tag="VFV93_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166364.1"
                     /translation="MVGTRDAGRGARDAKIIVGLHAPPKPPLYAMRAMVYAARALRLD
                     SFTVWDHFQDLFPEALWGTDFTWLARDSDSPHEMFDYQTLLGYLAAHAGRLRLGVTVT
                     EPVRRHP"
BASE COUNT          607 a         1174 c         1391 g          623 t
ORIGIN      
        1 gaccgggtaa caccatctcg gtcgcgctgg acatgcgatt ggcagggtat cgcggtgggg
       61 ggatcgcgcg gtacgcgcgg gagctgcggg cagccctggg tggggcatcc agcatcgcag
      121 ttcgcgggat tcggtcccaa agagatacag gcggcgcgga cggcgatctc cgtgtgagga
      181 cgccgccgca tcaccggctg gagcggttcg cgctgccggt ggagatggcg ctggcgcgct
      241 accagccaaa ggtgttccat gcgaccgatt tcatcgcgcc atccatgccg cgaagcgcgg
      301 ttgtcgcgac cgtccacgat ctggcattca tccattggcc gaacgatctg gcgccggatg
      361 cgctggagta ttaccggcag cttggggatg cgcggcggag gacggatgcc tggatcactc
      421 cctcacgctg gacggccgat gacctggcgg ggatctacgg gattgaccgg gacacgatcc
      481 atgtcatccc gcatggggtg tcgctcgatc tgatgcgggc ggatccgttg ccgcgcgtcg
      541 ggcggggcga ctacgtgctg gccgtcggga cggttgagcc gcgcaagcgc tacgacctgc
      601 tgctggatgc gatccaggag cgggacgatc tgccacgact ggtgatcgcc gggggatcgg
      661 gctggaatag ccagatgctg gaaaaccgct tgcggctggc ggctcgtgcc gagtgggttg
      721 ccgatgccgg cgacgacgcg ctacggcggc tctaccggga ggcgattgcg ctcgtcgtgc
      781 cgtcgcgggc ggaggggttc gggttgccgg cgctggaggc gatggcggtt gggacgccgg
      841 ttgtctcgtc aggcggcggg gcgttgccgg aggtgacggg cgaggcggcg cttgccgtcg
      901 cggaggcgag tggggaggcg tgggcggcgg cggtcgagcg gatcgcgagc gacaacgccc
      961 tctgggagca gctatcggcg gctgggcgcg tgcgggcgcg ccggttctcg tgggacgcgg
     1021 cggcgcgggc cacggcagag gtgtatcgca acctggtgac gtgacggtat actcccgcaa
     1081 tcgacacaga gtcacaggtg gcgtacgccg ctggagggta gagcggtggt gatcgacgag
     1141 caagtcatgt cgcgtgccca gggcgagccg gtctcgacct cggtcctgca cgtcatcatc
     1201 aatcccgccg ccggaagtgg ccgagcgggg cgacgctggc cggagcttga agcggagatg
     1261 ttccggcatg gctacgaggt caggccgagc ttcacgaccg gccccggcga cgcgatccgg
     1321 ctggcgcgcg agctggcagc gaacggcgcc cggacgatcg tctgtgtcgg cggcgatggg
     1381 acggtcaacg aggtcgtcaa cggcctgatc gacggcggcc ggcccgtcaa tcccgagaca
     1441 cggctggcag tcattccctg cggcaccggc aaggatctct gccggacgat cggcacgcgc
     1501 gatgtcacct cgatgctgcg ggcgctggcc gacgacacga cggcgctgat cgacctcggc
     1561 cgggtcagct gccgggacgc gaagaccggc gagccggtcg agcgctactt cgccaacgtc
     1621 gccgacacgg gaatcggcgc cgagacggcg gcccgcatca acgcctcgtc gaaggcgctg
     1681 ggcggcttcg tctcgtatct ctccggcgcg gttcgctcga tcgtggcctt caagccgtgg
     1741 caggtgacgg tcgaggtcga tggcgagccg gttcacagcg gctcagccgg gatggtcgtc
     1801 ttcgccaatg ggcgctactt cgccggcggg atgaacgtcg cgccggacgc ctcgctctgt
     1861 gatggcaagc tggacatctt cgtgctggaa ggggtcggca agcgggcgct gctgacctcg
     1921 ctgctgccac gcgtctatcg cgggcggcac gtcggccagc cgggggttgt ccacctgacc
     1981 ggcagccagg cgacggtgac gtgcgcggcg gggatgctgc tggagctgga tggggagcag
     2041 atcggaacga cgccgctagc ggtcgaggtg ctgccggcgg cgctgcggat cgttggcaca
     2101 caggacgcgt tggcagccgc aggtggttgc acggagcgcg ctgaataggc catagttgtc
     2161 acaagctcga ccggggtgcg aacgacagag cgctgaccag ccatcggctc ggccaggtca
     2221 agcattgcca tctgtcgtga atgaacgagc agacgagcaa cgttgcaaac aagtcagtgc
     2281 tgacaggggt tgtgctggga gttctctatg gtgtccacgc cgcatgagca ggtgagtacc
     2341 gcatacacaa acgacgggtt gggcgagcat ctggatgaga tgccgttcac gctgcatggc
     2401 aagccggcca gcgagttgca gaactggttc tggcaggggc tgaaggcgga gatgcaccgt
     2461 caggggcacg cctaccggcc cgagatcgtc gatgacatcc ggctggtcgt caacttcacc
     2521 cgtctggacg agccgcagcc gttccggcgc aaggcgcagg gcaccttcgt cgtcacgatc
     2581 gtcgaggaca ccagtgaccc ggccgacatc atccgggccg gctacccggt cctcgttcgg
     2641 gcgctttcga acctcggcat ctacctcgtg ccggtcggtg atcgggtgat cgcccacttc
     2701 ctgacgatgg agcaggggca ttacatcgtc gagctggccg aggacgaatc ggaagagcgc
     2761 ttcttcggcg aggtctacga acggctggcc ccgctggcga cctcccagct ggtcatcaac
     2821 aacgagtatc tgcccgacct gccggccgcc ctctgggacg gcgacgagat cacccgccag
     2881 ctgacctggg ccggtgagca gctcggcgat ctggcgctgc tgccggcgcc gtggccgatt
     2941 caggagattc tcagcgaccg cgacctgaag cacgtcatgc ggctgttcgg gatcggcggc
     3001 ctctcgtacg gcaacctctc ggcccgctac gacgatcagc gcttctggat gagcgccagc
     3061 ggcgtcgaca agaccaacct gcgcgagatc gggcgtgaca ttctgatggt gagcgactac
     3121 gttcgcgagc ggaatgcgat ggttctcagt gtcccgccgc acatccagcc gcggcgcgtc
     3181 tcggtcgacg ccatcgagca tttcatgatc taccgcgagc atccgaccgt cggcgcgatc
     3241 attcacatcc acgcctggat ggacgggatc gactcgaccc acatcaactt cccctgcggc
     3301 acccgcgagc tggcggtggc cgtctccgac ctggttcgca acgcaccgga cccctcgcgg
     3361 gccgtcgtcg gcctcaagaa ccacggcatg acactgaccg gccactcgct gccggagatc
     3421 ttcgagcgga ttgaggggaa gatcattccg caggttccga tgtcgtaaat ggtcgggacg
     3481 cgggacgcgg ggcgcggggc gcgggacgcg aagatcatcg tagggctgca tgcgccgccg
     3541 aagccgccgc tgtatgcgat gcgggcgatg gtgtatgccg cgcgggcgct gcggcttgat
     3601 tcgttcacgg tctgggacca ctttcaggat ctgtttcccg aggcgctctg ggggacagac
     3661 ttcacctggc tggcgcgcga cagcgactcg ccgcacgaga tgttcgacta ccagacgctg
     3721 ctgggctatc tggcagccca cgccgggcgg ctgcggctgg gcgtgacggt gacggagcca
     3781 gtccgccggc atccg
//
LOCUS       DASUGJ010000585         9544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2041390, whole genome shotgun sequence.
ACCESSION   DASUGJ010000585 DASUGJ010000000
VERSION     DASUGJ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041390"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VFV93_13270"
     CDS             <1..671
                     /locus_tag="VFV93_13270"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEX5166365.1"
                     /translation="LVLGTTLETACSWLGLIERSYERDGRVPVLRVTPFGRWIAGKRV
                     EPQLTQYGNAPIAVGANFQILLYRPTPRRVWALSAFAELQSLDRVSTWTLTAEALIRA
                     LAAGIDLDQITAFLERQNTAPLPQNVTYTLAEWDRGYRRVWLRRSVVLVPEDGEKAEP
                     IAAALKEAGLEPEVLSDGRLLLIYDEPDAGERLYTAATRALRERGFAPLADPQALPAR
                     RRSR"
     gene            757..1554
                     /locus_tag="VFV93_13275"
     CDS             757..1554
                     /locus_tag="VFV93_13275"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5166366.1"
                     /translation="MATIQIDGGRTVNYNVNGDGPPLLLIRGLGGAGADWGETVDRLS
                     PHFRVITFDNRDAGLNEPETAGYAMADMADDAAGLLRALGIDRAHVIGHSMGGFIAQH
                     LVLNHPDVVDHLVLVGTSPAAGAALGQPLSPPTAAEWIDDPAERARARAPLTHGPGYF
                     DNGNHADELDEIARISRTNRITHAGYSRQIAAISDTHDVRERLGEISAPTLVAHGDAD
                     PLVSLRGGQLLADGIPGAILKVYPGVGHYPYREAASDFYKDLAEFLG"
     gene            1920..2273
                     /locus_tag="VFV93_13280"
     CDS             1920..2273
                     /locus_tag="VFV93_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166367.1"
                     /translation="MTQVGKLLKGPLAFTIPLIAVAMVAVIGIGIGLLFIEVTHASNG
                     DVALITAVVLTAVIMAVAALLSYRDYKNPQAAETRTGGPSPRPTGESQAGPRAPVTTR
                     PVRGRSRSDGRRKSR"
     gene            2251..2706
                     /locus_tag="VFV93_13285"
     CDS             2251..2706
                     /locus_tag="VFV93_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166368.1"
                     /translation="MAAASPDSRDEQIAALAGQFVAYLRQGTDVELDAPGAVSDAHGP
                     AALLDAVERLDLALAQLVDLASVYDDGASMSLESLVLPAAALMGEYLRVCSGASWLAP
                     DPDDPAPDDSLAIVTSDGIAIDLLGAARATLLSGAPNLRSVVERLLATS"
     gene            2730..2978
                     /locus_tag="VFV93_13290"
     CDS             2730..2978
                     /locus_tag="VFV93_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166369.1"
                     /translation="MVVPPIWRDENPRDEHQRQEIVARNVRGNQRRARLLVACGVLLL
                     LCMLPAILVGLPGLGLILVPTGLFMIIGGAIQLLFERG"
     gene            3127..3798
                     /locus_tag="VFV93_13295"
     CDS             3127..3798
                     /locus_tag="VFV93_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166370.1"
                     /translation="MTDSRPTISSAAGEQPVEPRPTTGDPMHDMRTEVNAYAGAILVK
                     LLAIVNDAPVGSSGDVQRIEREIREMLAFVDPQLAADLGDLPLGDSVHGEAADPDRDR
                     RSAAPVDERIETLLNQLQQADVQAEASIELLRDLRMAIYSLRDDLRTQHDQTTEALQS
                     LTSTTRSLHYTITALSARSEQMTDLSGVAQEQIRVGVWKYALIAGISSGAIVAAIVAF
                     LSLVV"
     gene            3869..4819
                     /locus_tag="VFV93_13300"
     CDS             3869..4819
                     /locus_tag="VFV93_13300"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5166371.1"
                     /translation="MRRLALAALEHYDLHVRRLAIVSYHLNVLYRVETTSGERYALRI
                     SHPTWRDTVELRSEVAWLDALARETDIGAHRPLQNRDGALITTVAVEGVPEPRRAVLF
                     TWAPGVQLAERLTDENVEQMGVLSARLHQHGAAWVPPEGFTRRRLNRLFPRDEAVEIF
                     SPRFAHLFSPERRAVFEQGIERAQAELDRLYGSADPPRVVHGDLHHENVLIHRGQLQP
                     VDFEDIINAYPIQDIALTYYDFRYYTNPTTHDYATLCAAFQRGYTRQLPWPQEYENQI
                     NILHLARRIWITNWVLTRESPEHHAPFIEREAERFRALME"
     gene            complement(4937..5914)
                     /locus_tag="VFV93_13305"
     CDS             complement(4937..5914)
                     /locus_tag="VFV93_13305"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX5166372.1"
                     /translation="MAGLYDELTIFGGNSNTALTQEICDYIDVPVGRLDILKFSNENI
                     FVRIGESVRENDVFVVQSLTSPVSDSIMELLILIDTLKRSSSGRITAVIPYYAYGRTD
                     KKDQPRVPITARLLADMITVAGANRVLTIDLHAGQIQGFFNIPVDEMSAMPILVRYFA
                     EKRLRNPVVVSPDLGNTKRARNFAEGVDASLAVIEKRRVGNADQSEVLNLIGSVEGSP
                     AILVDDEIDTAGSIVQAARVCIENGATEVYACCTHGILSGPAIERLQTSPIREVVMTN
                     TVPIPPSKRIDKLTVLSVASLIGETIQRIHTGASVSLTYRAVDRVTNMR"
     gene            complement(5898..6764)
                     /gene="rnc"
                     /locus_tag="VFV93_13310"
     CDS             complement(5898..6764)
                     /gene="rnc"
                     /locus_tag="VFV93_13310"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX5166373.1"
                     /translation="MIADRGTDGSELVTTDELTSTEPAPNATDQDEEFHVDLSDLERD
                     DGRVERAMEALGVEFRNPKLMRLALVHRSFLNERGADAQTVIDQSNERLEFLGDSLIG
                     FFSAEWVYRHYPDQPEGVLTAHRVSLVRTETLAEWARGFGLHDLTYMARGELGPNGEV
                     RARLLAGSLEAALGAIYLDRGMTVARRFVHRLFTADASRRIEESEATNYKGRLQELIQ
                     ERERVTPGYRTVKASGPAHDREFVVEAVLRGRPLGSGTGPNKRAAEQEAARNALERLA
                     LEGIGDLDGRTV"
     gene            complement(6786..7226)
                     /locus_tag="VFV93_13315"
     CDS             complement(6786..7226)
                     /locus_tag="VFV93_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166374.1"
                     /translation="MADDVEKLLRFIAHRYVAREEILEIERDATAADEGHEIVADQRR
                     AIQSEEQRALEMADAFREGLRRADAARRAGGNAISLDDRKSEDDRIADALIHFLVRTG
                     IASSTTRETDQHRYIYTISVDWNALDRVSHDAKVNLGDLLGRGG"
     gene            7465..8742
                     /gene="fabF"
                     /locus_tag="VFV93_13320"
     CDS             7465..8742
                     /gene="fabF"
                     /locus_tag="VFV93_13320"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5166375.1"
                     /translation="MSQWGENGRRRVVVTGLGAYTSLAGTAEESWQRALAGESGISRI
                     ERWDPANFELPVGIGGEIKATPELPWLDSREARNIARFARYAVVAAAEAMGDAGLSAE
                     AANYDPVRAGAIVGTAAGGMDVIAETTRTFDARGYRRISPFFMVSFPHNMGTYHVAQA
                     FRLLGPSSAVSTACATGAQAIADAAEFIRRDQADVMLAGGAEHAVFPLFIASFVVQKA
                     ATTRPGDPAEASRPFDGEREGFVIGDGAGMLILEELEHARARGATIYAELLGTGSSND
                     GYHPIAPEPEGRGAAHAIGMALADAGIGPEQIDYVNAHAASTPLGDAAETAAIKRALG
                     EEHARQIPISSTKSMIGHTMGAAGAIEAIMTVLSIRDNRVHPTINQQTPDPACDLDYV
                     ANESRQVEVNIALKNSFGLGGQNAALIFGKWVG"
     gene            8863..9492
                     /locus_tag="VFV93_13325"
     CDS             8863..9492
                     /locus_tag="VFV93_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEX5166376.1"
                     /translation="MIAEFPLTGRVARRAAQVRPTQGQAFEVKEGQFIQISDMLGKQV
                     AVCVGFNLNDFDETLSPAHTRMGNNALMLREGHSLFSNRHNRMLTIIADSVGRHDMLV
                     PACNPQRYVEDYGISEHASCTDNMLRALTARKYTIEPDRLPDPINFFMNVGFKAGGEL
                     DIREPLSGRNDNILLKAHMDCLLVISACPNEQNAMNGYKPTDILVRVYQ"
BASE COUNT         1613 a         3169 c         3218 g         1544 t
ORIGIN      
        1 cgctcgtgct ggggacgacg ctggagacgg cctgtagctg gctcgggctg atcgagcgct
       61 cctacgagcg ggacggcagg gtgccggtcc tgcgggtgac gccattcggg cgctggatcg
      121 ccggcaagcg ggttgagccg cagctgacac agtacggcaa cgcgccgatc gctgttggcg
      181 ccaacttcca gatcctgctc taccggccga cgccgcgccg ggtctgggcg ctctccgcct
      241 tcgccgaatt gcaatcgctg gaccgcgtct cgacctggac gctgacggcc gaggcgctga
      301 tccgggcgct ggcggccggc atcgacctcg accagatcac ggccttcctc gaacggcaga
      361 acacggcgcc gctgccgcag aacgtgacct acacgctggc agagtgggat cgtggctacc
      421 gcagggtctg gctgcgccgc tcggtcgtcc tcgtcccgga agatggcgag aaggccgagc
      481 cgatcgccgc cgccctcaag gaggccgggc tggagccgga agtgctctcc gacgggcgac
      541 tgctgctgat ctacgacgag ccggacgccg gtgagcggct ctacacggcc gcgacccgtg
      601 ccctgcgcga gcgcgggttc gccccactgg ccgacccgca ggcgctgccg gcccgtcgcc
      661 ggagccggtg atacgcgcgt tcccgcgtgc tacgatgcgc gccgcatgac attgaagcag
      721 acgccgggcg cggcacgagc gagacgaggg caggcgatgg caacgattca gatcgacggt
      781 gggcggacgg tcaactacaa cgtcaacggc gacggtccgc cgctgctgct gatccgcggt
      841 ctcggtgggg ccggcgctga ctggggcgag acggtggacc ggctgtcgcc gcactttcgg
      901 gtgatcacgt tcgacaaccg cgacgccggc ctgaacgagc cggagacggc cggctacgca
      961 atggccgaca tggccgatga cgcggccggg ctgctacggg cgctgggaat cgatcgagcg
     1021 catgtcatcg gtcattcaat gggcggcttc atcgcccagc atctggtgct gaatcatccc
     1081 gacgtggtcg atcacctggt cctggtcggc acctcgccag cggccggcgc tgccctcggc
     1141 cagccgctgt caccaccgac cgccgccgag tggatcgacg atccggccga gcgcgcgcgt
     1201 gcccgagcgc cgctgacgca cggccccggc tacttcgaca acggtaacca cgccgatgaa
     1261 ctggacgaga tcgcccgaat ctcgcgaacc aaccggatca cgcacgcggg ctactcgcgc
     1321 cagatcgccg ccatcagcga cacccacgat gtgcgcgagc ggctgggcga gatcagcgca
     1381 ccgacactgg ttgcccacgg cgacgccgat ccgctcgtct cactgcgtgg cggtcagctg
     1441 cttgccgacg gaatccctgg cgcgatcctc aaggtctacc caggcgtcgg ccactacccc
     1501 tatcgagaag ccgccagcga tttctacaag gatctggcag agtttctcgg gtagaggctg
     1561 gggactgact gagagttgtc acgtcgtaga ggacccaacc tggtcccccg tcgtcccgaa
     1621 tagtccgcca ccgaaccttg gtgggaaaac gggcttcccc tgtcgtcctg agccgcaacg
     1681 aaggacctct cctgtcttga acgcccgtgg gcatgtcgct gggaggccct tcgctgcggc
     1741 tcagggcgac aggggaggga tgccggtcct cgggaacgca tcaactctgg aatgcggccg
     1801 gatagcctcc tcgcttatca tcaccgctag cgcaaacggc gttggagcgg gtaagatgcg
     1861 gaggggcggc gcgtggctcg gcgcgaggct caggcagggt atctagccga cgaggagggg
     1921 tgacgcaggt gggcaagctg ctcaaggggc cgctggcatt cacgattccg ctgatcgcgg
     1981 tggcgatggt tgcggtgatc gggatcggca tcggactcct gttcattgag gtgacgcacg
     2041 cgtccaacgg cgacgtggcg ttgatcaccg ccgtcgtgct gacggctgtc atcatggcgg
     2101 ttgcggcgct gctgtcgtat cgcgactaca agaacccgca ggctgccgag acgcggaccg
     2161 gcggtccgtc gccccgccca actggcgagt cgcaggcggg accgcgcgca cccgttacca
     2221 cccggcccgt ccgtggccgg tcgaggtcgg atggccgccg caagtcccga tagccgcgac
     2281 gagcagatcg cggcgcttgc agggcaattc gtcgcctatc tgcgtcaagg gaccgatgtc
     2341 gagcttgatg cgcccggggc cgtgagcgac gctcacggcc ccgccgcgtt gctggacgcc
     2401 gtcgaacggc tcgatctggc gctggcgcaa ctggtcgacc ttgcctcggt ctacgacgac
     2461 ggcgctagca tgtcgctgga gtcgctggtc ctgccggccg ccgcgctgat gggtgaatac
     2521 ctgcgcgtgt gcagcggcgc gagctggctg gcgcccgatc cggacgaccc ggcgccggac
     2581 gactcgctgg cgatcgtcac cagcgatggc atcgcgatcg atctgctggg cgccgcccgg
     2641 gcaacgctgc tgagcggcgc gccgaacctg cgctcggtcg tcgagcggct gctggcaacc
     2701 agctagccac cggttctggg aggccaccta tggtcgtgcc gccgatctgg cgagacgaga
     2761 acccgcgcga cgagcatcag cgccaggaga tcgtcgcgcg gaacgtgcgc ggcaaccagc
     2821 ggcgtgcccg tctgctggtg gcctgcggcg tgctgctgtt gctctgcatg ctgccggcga
     2881 tcctggttgg cctgccgggg ttggggctga tcctcgtgcc aaccgggctg ttcatgatca
     2941 tcggcggcgc aatccagctc ctctttgagc gcggctgagc ggactggtcg acagacgagt
     3001 cgcaatggcc gggctgaatt tcaggcaatc tcgaaccaaa tggatacgtt gtacgttaaa
     3061 caaggtagaa ttcgatcggg tgcagcgacg ttatgacgac attcgcgagc tggaggcccg
     3121 acactggtga ccgatagcag gccaaccata tcgtccgccg ctggtgagca gccggtcgag
     3181 ccacgaccga ccaccggcga cccaatgcac gacatgcgca ccgaggtcaa tgcctacgcg
     3241 ggcgcgattc tggtcaagct gctggcgatc gtcaacgacg cgccggtcgg gagcagcggc
     3301 gacgtccagc gcatcgagcg cgagattcgc gagatgctgg ccttcgtcga tccgcagctg
     3361 gcggccgacc tgggcgatct gccgcttggc gacagcgtcc atggcgaggc cgccgatccg
     3421 gatcgcgatc gacgatcggc cgcaccggtt gacgagcgga tcgagacgct gctgaaccag
     3481 cttcagcagg ccgacgtgca ggcggaggcg tcgatcgagc tgctgcgcga cctgcggatg
     3541 gcgatctact cactgcgcga cgatctgcga acgcagcatg accagacgac cgaggcgctg
     3601 caatcgctga cctcgacgac ccgcagcctg cactacacga tcaccgccct ctcggcccgc
     3661 tcggagcaga tgaccgacct gagcggagtc gctcaggagc agatccgcgt cggcgtctgg
     3721 aagtacgcgc taatcgccgg catcagcagt ggcgcgatcg tcgctgccat cgtcgccttc
     3781 ctcagtcttg tcgtctaagc gcggcgagcc cgcacaacga gcactgcgtc cattcgagca
     3841 actgtccagc cggggccggc ttggacgggt gcgccggctc gcgctggccg cgctggaaca
     3901 ctacgacctg catgtccggc ggctggcgat cgtcagctac catctgaacg tcctctaccg
     3961 cgtcgagacg accagcggcg agcgctacgc gctacggatc agccatccga cctggcggga
     4021 taccgtcgag ttgcgctcgg aggtcgcctg gctcgacgcg ctggcgcgcg agacggacat
     4081 cggtgcccac cggccgctgc agaaccgtga cggcgcgcta atcacgaccg tcgccgtcga
     4141 aggcgtccct gaaccacggc gcgcggttct gttcacctgg gcaccgggcg tccagctcgc
     4201 cgagcggctg acggatgaga acgtcgagca gatgggagtc ctctcggcgc ggctccatca
     4261 gcacggcgcc gcttgggtac cgccggaggg gttcacgcgg cgacggctca accggctctt
     4321 tccccgcgat gaagcggtag aaatcttctc acctcgcttc gcgcacctct tctcgccaga
     4381 gcggcgcgcg gtcttcgagc agggcatcga gcgggcgcag gcagagctgg accggctcta
     4441 cggcagcgcc gatccgccac gggtcgtcca cggcgacctg caccatgaga acgtcttgat
     4501 ccaccgtggg caactccagc cggtcgactt cgaggacatc atcaacgcct acccgattca
     4561 ggacatcgcg ctgacctact acgacttccg ctactacacc aacccgacca cccacgacta
     4621 cgctaccctc tgtgccgcct tccagcgtgg ttacacgcgc cagctcccct ggccgcagga
     4681 gtatgagaac cagatcaaca tcctccacct cgcccgccgg atctggatca ccaactgggt
     4741 cctgacgcgc gaatcacccg agcaccacgc cccgttcatt gagcgcgaag cagagcggtt
     4801 cagggcgttg atggaatgaa ctcattggaa aatgtggatt agcgttcgcg atcaggcggc
     4861 ttgatatctc agcaccgaac gccgcgagtg gagtgatcgg gactgcgccg tacgagtcct
     4921 gcatcgttcc atcgcctcag cgcatgttgg tgacgcggtc gacggctcgg taggtgaggg
     4981 agacgctggc gccggtgtgg atgcgctgga tcgtctcacc gatcaggctg gcaaccgaga
     5041 ggacggtcag cttgtcgatc cgcttgctgg gcgggattgg gacggtgttg gtcatgacga
     5101 cctcgcggat cggcgaggtc tggaggcgct cgatggcggg gccgctgagg atgccgtgcg
     5161 tgcagcaggc gtagacctcg gtcgcgccgt tctcgatgca gacgcgggcc gcctgaacga
     5221 tcgagccggc cgtgtcgatc tcgtcgtcga cgaggatagc cgggctgccc tcgaccgagc
     5281 cgatcaggtt gagcacctcg ctctggtcgg cgttgccaac ccgccgcttc tcgatgacgg
     5341 ccagcgaggc gtcgacgccc tcggcgaagt tgcgcgcccg cttggtattg ccgaggtctg
     5401 gcgagacgac gaccgggttg cgcagccgct tctccgcgaa atagcgcacc aggatcggca
     5461 tggccgacat ctcgtcgacc gggatgttga agaagccctg gatctggccg gcgtggaggt
     5521 cgatcgtcag aacccggttg gcgccggcga cggtgatcat gtcggccagc agccgggccg
     5581 tgatcggcac gcgcggctga tccttcttgt cggtgcggcc ataggcgtag tagggaatga
     5641 cggccgtgat ccggccggac gacgagcgct tgagcgtgtc gatcaggatc agcagctcca
     5701 tgatcgaatc ggagacgggc gaggtcagcg actggacgac gaagacgtcg ttctcgcgaa
     5761 cgctctcgcc gatccggacg aagatgttct cgttcgagaa cttgaggatg tcgagccgac
     5821 ctaccggaac gtcgatgtag tcgcagatct cctgggtgag ggcggtgttc gagttcccgc
     5881 cgaagatggt gagctcgtca tacagtccgg ccatcgaggt cgccgatccc ttccagcgcc
     5941 aggcgctcga gtgcgttgcg ggccgcttcc tgttcagcgg cacgtttgtt cgggccggtt
     6001 cccgatccga gtggccggcc gcgcaggacg gcctcgacga cgaactcccg gtcatgggcc
     6061 gggccggacg ccttgacggt ccggtagccg ggcgtcaccc gctcccgctc ctgtatcagc
     6121 tcctggaggc ggcctttgta gttggtcgcc tcgctttctt cgatccggcg gctggcgtcg
     6181 gcggtgaaga gtcggtggac gaagcgccgc gcgaccgtca tgccccggtc gaggtagatc
     6241 gcgccgaggg cggcttcgag cgagccggcc agcagccgcg cccgcacctc gccattcggc
     6301 cccagctcgc cgcgcgccat gtaggtcaga tcgtgcagcc cgaagccgcg cgcccactcg
     6361 gccagcgtct cggtccggac cagcgagacg cgatgggccg tcaggacgcc ttcaggctga
     6421 tcagggtaat gtcgatagac ccactcggcg ctgaagaaac cgatcagtga gtcaccgagg
     6481 aactccagcc gctcgttgga ctggtcgatg accgtctgcg catcagcgcc gcgctcattc
     6541 aggaacgagc ggtggacgag cgccagccgc atcagcttcg gattgcggaa ctcgactcca
     6601 agcgcctcca tcgcccgctc gacgcggccg tcatcgcgct ccaggtcgga gaggtcaacg
     6661 tgaaactctt cgtcctggtc cgttgcgttt ggggctggtt cggtggaggt cagttcgtcg
     6721 gtcgtcacaa gctcgcttcc gtcagttcct cggtcagcga tcacacaggt catcattgca
     6781 cgcggctagc caccgcgccc gaggagatca ccgaggttga ccttcgcgtc atgtgagaca
     6841 cggtcgagag cgttccagtc gacggagatg gtgtagatgt agcggtgctg atccgtttcg
     6901 cgggttgtcg atgaggcgat accggtccgg acgaggaagt ggatcagcgc atcggcgatg
     6961 cggtcgtcct ccgacttgcg atcgtcgagc gagatggcgt tgccgcccgc gcgccgggcc
     7021 gcgtcggcac gccgcaaccc ctcgcggaag gcgtcggcca tctccaacgc gcgttgctcc
     7081 tcgctctgga tcgcgcggcg ctggtcggcg acgatctcat gaccttcgtc ggcagccgtc
     7141 gcgtcgcgct cgatctcgag gatctcttca cgcgcgacgt agcgatgggc aatgaaccgc
     7201 agcaattttt cgacgtcgtc ggccacgcgc ccctcattca ttgtcccggc ttgccggcgt
     7261 cgttcgcccg ctcaggaatc gtcaggctcg ccgcaccccc acgatgaacg gcaacgagcc
     7321 atgacccagc cgcgattata ggcgagtcca gaccgcacgc caattggcgc tgtgccttgc
     7381 cggcaccgat caatctggta cgatggcatg ccggattgag cgaagcgatc gacccagcag
     7441 acgggcaatg aacgggatat ccgcgtgagt cagtggggtg aaaacggacg gcggcgcgtc
     7501 gtcgtgaccg gccttggcgc gtacacgtcg ctggccggaa cggccgagga atcgtggcaa
     7561 cgggcgctgg ccggcgaatc gggcatcagc cgaatcgaac gctgggaccc ggcgaacttc
     7621 gagttgccgg tcggcatcgg cggcgagatc aaggcgacgc cggagctgcc ctggctggac
     7681 agccgcgagg cacgcaacat cgcccgtttc gctcgctacg cggtcgtcgc cgccgccgaa
     7741 gcgatgggtg atgccggtct ctcggccgaa gccgccaact acgatcctgt ccgggccggc
     7801 gcgatcgtcg ggacggccgc cggcgggatg gatgtcatcg ccgagacgac gcggacgttt
     7861 gacgcgcgcg gctaccggcg catctcccca ttcttcatgg tcagcttccc gcacaacatg
     7921 ggcacctatc acgtcgcgca ggcgttccgg ctgctggggc cgagctcggc cgtctcgacc
     7981 gcgtgtgcga ccggcgccca ggcgatcgcc gacgcggccg agttcatccg gcgcgaccag
     8041 gcggatgtca tgctggccgg tggggcggag cacgccgtct tcccgctctt catcgcctcg
     8101 ttcgtcgtcc agaaagcagc cacgaccagg cccggggacc ccgccgaggc cagccgaccg
     8161 ttcgacggcg agcgcgaggg gttcgtgatc ggcgacggcg ccgggatgtt gatcctcgaa
     8221 gagctggagc acgcacgggc acggggcgcg acgatctacg ccgagctact cgggaccggc
     8281 tcatcgaacg acggctacca cccgattgcc ccggagccgg aggggcgcgg tgcggcccac
     8341 gcgatcggga tggcgctcgc cgacgccggg atcggacccg agcagatcga ctacgtcaac
     8401 gcccacgccg cctcgacgcc gcttggcgac gccgccgaga cagccgcgat caagcgcgcg
     8461 ctcggcgagg agcatgcccg ccagatcccg atctcctcga ccaagtcgat gattggccac
     8521 acgatgggcg cggccggcgc gatcgaagcg atcatgacgg tcctcagcat ccgcgacaac
     8581 cgggtccacc caaccatcaa ccagcagacg ccggacccgg cctgcgacct cgactatgtc
     8641 gccaacgaat cgcgccaggt cgaggtcaac atcgcgctga agaactcatt cggcctcggc
     8701 ggccagaacg ccgcgttgat tttcggtaag tgggtgggct aacccctccc cccaaccccc
     8761 tcccctatgc ggagagggga gccaggtacg gggtggccaa ctcgacacgt ttcgccaaac
     8821 tccccctctc cgcatagggg agggggttgg ggggaggggt tagtgatcgc agaatttcca
     8881 ctgacaggac gggtagcgcg acgggcggcc caggtgcggc cgacgcaggg gcaggcgttc
     8941 gaggtcaagg aggggcagtt catccagatc agcgacatgc tcggcaagca ggtcgccgtc
     9001 tgtgtcggct tcaatctgaa cgacttcgac gagacgctct cgccggccca cacgcggatg
     9061 ggcaacaacg cgctgatgct gcgcgagggg cacagcctgt tctccaaccg gcacaaccgg
     9121 atgctgacga tcattgccga cagcgtcggg cggcatgaca tgctggtccc ggcctgcaac
     9181 ccgcagcgct acgtcgagga ctacggcatc agcgagcacg cgagctgcac ggacaacatg
     9241 ttgcgggcgc tgacagcccg caagtacacg atcgagccgg accgcctacc cgacccgatc
     9301 aacttcttca tgaacgtcgg cttcaaggct ggcggcgagc tggacatccg cgaacccctc
     9361 tccggccgca acgacaacat cctcctcaaa gctcacatgg actgcctgct ggtcatctcc
     9421 gcctgcccca acgaacagaa cgccatgaac ggctacaagc cgacggacat tctggtgcgg
     9481 gtgtaccagt agcgtcggga accgggattc gggattcggg gcaggagaac cgagcgcatc
     9541 gttt
//
LOCUS       DASUGJ010000586         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1763517, whole genome shotgun sequence.
ACCESSION   DASUGJ010000586 DASUGJ010000000
VERSION     DASUGJ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763517"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFV93_13330"
     CDS             <1..194
                     /locus_tag="VFV93_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166377.1"
                     /translation="VVRVFDFTIDDAGWSMIRLDDEFSQRYTAQFRGPDVMESAGEAS
                     FDRGASWRPDFTMTYQRVP"
     gene            236..>2299
                     /locus_tag="VFV93_13335"
     CDS             236..>2299
                     /locus_tag="VFV93_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5166378.1"
                     /translation="MSVATRSETTSTTLHDADSHDVIRVHGARENNLKDISIAIPKRR
                     LTVFTGVSGSGKSSLVFGTIAAESQRLINETYSAFVQGFMPTLARPEVDVLDGLTTAI
                     IVDQERLGANPRSTVGTVTDANAMLRILFSRLGQPHIGPPNAFSFNVASVTASGAITV
                     DRGGRTKTEKATFSRLGGMCPRCEGRGSVTDFDLSQLYDDSKSLNEGALTIPGYSMDG
                     WYGRIYRGCGFFDPDKPISKFNKRELHDLLYKEPTRIKVDGVNLTYAGLIPQIQKSYL
                     SKDREAMQPHIGAFVDRVVTFTTCPDCDGTRLSAAARSSKIDEISIADACAMQISDLA
                     GWVAGLDAPSVGPLLAALRHSLDSFVEIGLGYLSLNRPSGTLSGGEAQRVKMIRHLGS
                     SLTDVTYVFDEPTTGLHPHDIQRMNELLLQLRDKGNTVLVVEHEPEMIEIADHVIDLG
                     PGAGTFGGEVVFEGTVEGLRASGTLTGRHLDDRAALKPSVRKPSDALEVRGANAHNLR
                     DIDVDIPLGVLVVVTGVAGSGKSTLIHGSVSGRDGVVAIDQTPIRGSRRSNPATYTGL
                     LDPIRKAFAKANGVKPALFSANSEGACPTCNGAGVIYIDLGMMAGVTTVCEECEGKRF
                     QAEVLTYTFGGKDISEVLAMSVNQAREFFGDGEAKTPAAHKILTNLADVGLGYLSLGQ
                     PLTTLS"
BASE COUNT          431 a          781 c          726 g          361 t
ORIGIN      
        1 gggtcgtccg tgtcttcgat ttcacaattg acgacgctgg ctggtcaatg atccgcctgg
       61 acgacgaatt ctcccagcgc tacacggcac aattccgtgg accggatgtg atggagagcg
      121 ccggcgaggc atcgttcgac cgaggcgcca gctggcggcc cgacttcacg atgacctacc
      181 aacgcgtgcc gtaaaccgac gaagaccacc tggccgatga acagatggag agatgatgag
      241 cgtagccacg agatcagaga cgacgtcaac gacgctgcac gatgccgaca gccatgatgt
      301 gatccgcgtg catggtgcgc gcgagaacaa cctcaaagac atcagcatcg cgatcccgaa
      361 gcgccggttg acggtcttta ccggcgtctc cggctcgggc aagagctcgc tggtgttcgg
      421 gacgatcgca gccgagtcgc agcgactgat caacgagacc tacagcgcgt tcgtccaggg
      481 cttcatgccg acgctggctc gtccggaggt cgatgtcctc gatgggctga cgacggcgat
      541 catcgtcgat caggagcggc tgggcgccaa cccccgctcg acagtcggca ccgtcaccga
      601 cgccaacgcg atgctgagga tcctcttcag ccggctcggg cagccgcaca tcggcccgcc
      661 caacgcattc tccttcaacg tcgcctccgt cacggccagc ggggcgatca cggtcgatcg
      721 cggcggcagg accaagaccg aaaaggccac attctcccgc ctcggcggca tgtgcccgcg
      781 ctgcgagggc cgcggctcgg tgaccgattt cgatctgtcg cagctgtacg acgacagcaa
      841 gtcgctcaac gagggtgcgc tgacgattcc cggctacagc atggacggct ggtacggccg
      901 catctaccgt ggctgcggct tcttcgaccc ggacaagccg atctccaaat tcaacaagcg
      961 cgagctgcat gacctgctct acaaggagcc gaccaggatc aaggttgacg gcgttaacct
     1021 gacctacgct gggctgatcc cgcagatcca gaagtcgtat ctctccaagg accgcgaggc
     1081 gatgcagccg cacatcggcg cgttcgtcga tcgggtcgtg accttcacga cctgccccga
     1141 ctgcgatggc acccgcctca gcgcggcagc ccgctcgtcc aagatcgacg aaatcagtat
     1201 cgccgacgcc tgcgcgatgc agatcagcga cctggccggc tgggtggccg gcctcgacgc
     1261 gccgtccgtc gggccactgc tggccgccct gcggcactcg ctcgactcat tcgtcgagat
     1321 cgggctgggt tacctctcgc tcaaccggcc gtccggcacg ctctccggcg gcgaggcgca
     1381 gcgcgtcaag atgatccggc acctgggctc atcactgact gatgtcacct acgtcttcga
     1441 cgagccaacg accggcctgc acccacacga catccagcgc atgaacgagc tgctcctgca
     1501 actgcgcgac aagggcaaca cggtcctcgt cgtcgagcac gagccggaga tgatcgagat
     1561 cgccgaccac gtcatcgatc tcggccccgg cgccgggacg ttcggcggcg aagtcgtctt
     1621 cgagggcacc gtcgaggggc tacgggccag cggcacgctg accgggcggc atctcgacga
     1681 ccgggcggct ctgaagccat cggtccgaaa accgtcggac gcgctggagg tacgtggcgc
     1741 gaacgcccac aatctgcggg acatcgatgt cgacattccg ctcggcgtgc tggtcgtcgt
     1801 caccggcgtg gccggctccg gcaagagcac gctgatccac ggttcggtct ccgggcggga
     1861 cggggtcgtc gcgatcgatc agacgccgat ccgaggctcg cggcgcagca acccggccac
     1921 ctataccggc ctgctcgacc cgatccggaa ggcgttcgca aaggccaacg gcgtcaagcc
     1981 ggccctgttc agcgccaact cagaaggcgc ctgcccgacc tgcaacgggg ccggcgtgat
     2041 ctacatcgac ctgggcatga tggccggcgt cacgaccgtc tgcgaggagt gcgaagggaa
     2101 gcgcttccag gccgaggtgc tcacctacac gttcggcggc aaggacatca gcgaggtgct
     2161 ggcgatgtcg gtcaaccagg cccgcgagtt cttcggcgac ggcgaggcga agacaccggc
     2221 tgcccacaag atcctgacca acctggccga tgtcgggctc ggctatctca gcctcggaca
     2281 gccgctgacg acactctcc
//
LOCUS       DASUGJ010000587         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_514007, whole genome shotgun sequence.
ACCESSION   DASUGJ010000587 DASUGJ010000000
VERSION     DASUGJ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514007"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1670
                     /locus_tag="VFV93_13340"
     CDS             297..1670
                     /locus_tag="VFV93_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5166379.1"
                     /translation="MQRVFTEGLAQASFVVASDATRQAIVIDPRRDVAVYLAIAAAHD
                     LQIAHIYETHIHADYASGARELAARTGAVMHLSAIGESEFEHEPVHDGDVLAVGELRV
                     QALLTPGHTPEHICLLVTDTAHPADPATLFSGDAVFVGSVGRPDLLGAEQTKALATAL
                     YQTIHDTLMTLDDEIIVYPGHGAGSACGKAIGNDPSTTMGQEKRFNFAFQPANEEQFV
                     EMILDGLPEPPPYFPVMKRLNKQGPQILGGIPSPDSLSTDDLRRAQQGETTIVDVRPA
                     ERFGEGFLAGSLNIGLGPSLPNWAGWAVSYERPIVLVVADAADVEEAVTWLIRIGLDN
                     VTGYAVADPDGWRAAGLAVERIEQIDPRTANARLAAGDLQLLDVRNIDEWNGGHVSGA
                     HNVPVGPLVRGADGLDPNRPVAVMCAAGYRSSLGASALKQRGYDDVINVAGGMDAWRA
                     AGLPVDA"
     gene            1709..2155
                     /locus_tag="VFV93_13345"
     CDS             1709..2155
                     /locus_tag="VFV93_13345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX5166380.1"
                     /translation="MELYVSEVSNEGTTLFEEEAGYTAIRNFEDLIAWQRARELTAEV
                     YMLARARGLRQDFGLRDQLQRAAVSVMSNIAEGHERGSSREYFRFLTIAKASCAEVSS
                     LLYVAHDAGYIDEDAFRRHLDTTRRVGQLVGALRSSISRRIDSQRR"
BASE COUNT          414 a          729 c          766 g          398 t
ORIGIN      
        1 ggacgacagg ggagctacgc gacaggtctc ctgtggtgga ccactcagga tgacagggca
       61 gggggtacgg atccccttgc acgccgtacg tctggtccgc gtcacgaacc ccatgccgcg
      121 atgcaggcgc cgatcatccg cagatcgcga tcggtcagga cgtctgacga acccgaagta
      181 taatgaagca atcggatatt cgtcggagct gaaagcgtgg ttcggcaggt cggggctgca
      241 ttgcggtccg gttcggccgc agggctgggg atgagaggcg gggactgacc atgttcatgc
      301 aacgcgtttt cacggaaggg ctggctcagg cgtcgttcgt cgttgcgtcg gacgcgaccc
      361 gtcaggcgat tgtcatcgat ccccgccgtg atgttgccgt ctacctcgcg atcgccgctg
      421 ctcatgacct ccagatcgca cacatctacg agacccacat ccatgccgac tacgcttccg
      481 gtgcacggga gctggccgcc cggaccgggg cggtgatgca tctgagcgcg atcggtgaga
      541 gcgaattcga gcacgagccg gttcacgacg gcgatgtcct ggcggttggc gaattgcgcg
      601 tacaggcgct gctgacgccc ggccacacgc ccgagcacat ctgcctgctg gtgaccgaca
      661 cggcccaccc ggccgacccg gcgacgctct tctccggcga tgccgtcttc gtcggtagcg
      721 ttggccggcc cgacctgctc ggcgccgagc agaccaaggc gttggcgacg gcgctctacc
      781 agacgatcca cgacacgctg atgacgctag acgatgagat catcgtctac ccgggtcacg
      841 gggcgggctc ggcctgcggc aaggcgatcg gcaacgaccc cagcacgacg atgggtcagg
      901 agaagcgctt caacttcgcc ttccagcctg ccaatgagga gcagtttgtc gagatgatcc
      961 tcgacggcct accagagccg ccgccctact tcccggtcat gaagcggctc aacaagcagg
     1021 ggccgcagat tcttggcggc attccctcgc ccgacagcct gagcaccgac gatctgcgtc
     1081 gggcgcagca gggcgagacg acgatcgtcg atgtccgtcc ggccgagcgt ttcggcgagg
     1141 gcttcctggc cggctcgctc aacatcgggc ttggcccaag cctgccgaac tgggccggct
     1201 gggccgtctc ctacgagcgc ccgatcgtgc tggtcgtcgc tgatgccgcc gacgtcgagg
     1261 aggccgtgac ctggctgatc cggatcggcc tcgataacgt caccggctac gcggttgctg
     1321 accccgacgg ctggcgtgcg gccggcctgg ccgtcgagcg gatcgagcag atcgacccgc
     1381 gcaccgccaa cgcgcgcctg gcggccggcg atctgcaact gctcgatgtc cgcaacatcg
     1441 acgaatggaa cggcggccac gtctccggcg ctcacaacgt cccggtcggc ccactcgtgc
     1501 gtggagcgga cggtctcgac cccaaccggc cggtcgccgt catgtgcgct gccggctacc
     1561 gcagtagcct cggcgccagc gccctcaagc aacgcggtta cgatgacgtc atcaacgtcg
     1621 ccggcggcat ggacgcctgg cgcgcggcgg ggttgccggt tgacgcgtag acggacggga
     1681 cgcgggacgc gggatgcggg acgcggattt ggaactctat gtgagcgaag tgtcgaacga
     1741 gggcaccact ctgtttgaag aggaggcggg atataccgct attcgaaact tcgaagacct
     1801 gatcgcgtgg cagcgtgcgc gagagttgac agcagaagtc tatatgttgg cacgagcgag
     1861 ggggctgcga caggacttcg gattgcggga tcaacttcaa cgcgcggccg tctcggtcat
     1921 gtccaacatc gcagaaggtc acgagcgagg ttcttcgcgg gagtatttcc gatttctcac
     1981 aatcgcaaag gcgtcatgcg ccgaagtcag ttcgctcctg tacgtcgcgc acgatgcggg
     2041 ctacattgat gaagatgcgt tccggcgcca ccttgacact actcgccggg ttgggcaact
     2101 ggtcggagca ttgcgctcga gtatctctcg cagaattgat agccagagac gctaatcgtc
     2161 ccgcgtcccg aaaacgaaag gaaccaccca tgtccaccac ctatcccaac caccgcccgc
     2221 ccgtgatggg gcgcaatggc atggtctcgt cggcgcatta tctggcgtcg atggctgggt
     2281 tgcgggtgat gatggagggg ggcaatg
//
LOCUS       DASUGJ010000588         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_930562, whole genome shotgun sequence.
ACCESSION   DASUGJ010000588 DASUGJ010000000
VERSION     DASUGJ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930562"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VFV93_13350"
     CDS             complement(<1..750)
                     /locus_tag="VFV93_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166381.1"
                     /translation="MLRQLWLLFRRKLLETIRQPIWVILGLTTPLLYLALFAPVLEPL
                     GGGPGFSSGDVLDVFVPGILALMAFGAGTGAGWIVISELEAGVIERLRVTPVSRFALV
                     LGPALRDTLTMLIPALIVVVVAVPFGFHADWTGIALLMLLLGMVTIVTSAASSALGLI
                     LLEVGSLAAIWNGVNLPILLLSGVLLPLSLAPGWMQLLAHLNPLYYVVEAARDLAAGT
                     MGSWVVLQGFLIMTLLSVVVLWWTTRTYRQAV"
     gene            complement(753..1748)
                     /locus_tag="VFV93_13355"
     CDS             complement(753..1748)
                     /locus_tag="VFV93_13355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5166382.1"
                     /translation="METQQAVFTHDLRKSFQVNGVMVAAVQGVDLEVSRGEIFGFLGP
                     NGAGKTTVLRMLTTLLPIDSGEARIGGYDVRRQPEDVRRCIGYVSQAGGSDPTATARE
                     NLILQGQLYGAATPVAARRADELIRFFELEPFADRAVRSYSGGQRRRLDVALGIVHQP
                     AVLFLDEPTTGLDPQNRANLWGQVHQLRDGGTTVFLTTHYLEEADALSDRLAIIDHGR
                     IVAEGTPQELKQRLAGEAITINPHGERIALEVLQRDLAALPSVRESRIEGKRLRLSVD
                     DGARALPSVIECLQRCGVALEAIALTAPSLDDVFLNQTGRTLRDAGNGVAEQMEQ"
     gene            1903..2541
                     /locus_tag="VFV93_13360"
     CDS             1903..2541
                     /locus_tag="VFV93_13360"
                     /inference="COORDINATES: protein motif:HMM:NF037675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5166383.1"
                     /translation="MIARDDERQEALGRRTPARDMRQRDAERSRELLLSAALDEFAAK
                     GFAGARVQDIASRAGLNKQLITYYFGGKEGLYQALERRWLDLESTFARPDIPIDELAV
                     EYLHAVLADPRLFRLLVWNGLAERGGPSDPADEDPVGHDDVADLLRRQQAGELASDLD
                     PGLVLLALMGAIAAPIVLPQVVRRVTGLDFRSPEFEQAYAEQLRRIVQHLAE"
     gene            2712..3860
                     /locus_tag="VFV93_13365"
     CDS             2712..3860
                     /locus_tag="VFV93_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5166384.1"
                     /translation="MQETIVNPFAGLDLAQLHTRTCAKWSVYPDDVLPLWVAEMDAVI
                     AQPIADAVMAALANGDTGYPYGRAYPEALASFASDRWGWSFDTASTAMVADVMSGVRE
                     VSRAIGAADAPVVVTPPVYPPFFGVAKTLRRPVVEAPLDAQGRLDTAALEAAFKEATA
                     GGRKAIMLLCNPHNPTGTVHSRSELEMVARLARQYGVRVVSDEIHSPLIMPTATFTPY
                     LSVEGTGSDFAVVSASKGWNLAGFKAAVVVAGPDATDELKSFGFHGAGHIAVIAHTAA
                     FNQARDWLDGAIAGIDANRKLLADLLETHLPSTVYQMPEATYLAWIDCRDLGLGDDPT
                     AAFLERSKVALSSGTPFGSQGVGFVRLNLATSPEILTEAVTRMADVRG"
BASE COUNT          700 a         1372 c         1319 g          617 t
ORIGIN      
        1 gaccgcctgg cggtaggtgc gcgtcgtcca ccagaggaca acgacgctga ggagcgtcat
       61 gatcaggaag ccttgcagga ccacccagga acccatcgtg ccggccgcga ggtcgcgggc
      121 agcctcgacg acgtagtaga gcgggttcag gtgggccagc agctgcatcc agcccggcgc
      181 gagcgagagg ggcagcagca cacccgacaa cagcaggatc gggaggttga cgccgttcca
      241 gatcgcggcc aggctgccga cctcgagcag gatcagcccc agcgcgctgg acgccgccga
      301 ggtcacgatt gtcaccatcc cgagcagcag catcagcagc gcgatccccg tccagtccgc
      361 atggaatccg aaaggaacgg cgacgacgac gacgatcagc gcggggatga gcatcgtgag
      421 tgtgtcgcgc agggccggcc ccagcaccag cgcgaagcgg ctgaccggcg tcacccgcag
      481 ccgctcgatg acacccgcct ccagttcgga gatgacgatc cagccggcgc cagtcccggc
      541 gccgaacgcc atcagcgcca gaatgccggg gacgaagaca tccagcacgt cgccactgct
      601 gaagcccggc ccgccgccga gcggctcgag gacgggcgcg aaaagcgcca ggtagagcag
      661 cggggtggtc aggccgagga tgacccagat tggctgccgg atcgtctcaa gcagcttgcg
      721 acggaagagc agccagagtt gtcgcagcat ggtcattgct ccatctgctc cgcaacgccg
      781 ttaccggcat cgcgcagcgt tcgaccggtc tgattgagga aaacatcgtc gagcgacggc
      841 gccgtcagcg cgatcgcctc aagcgcaaca ccgcaccgct gcaggcactc gatgaccgac
      901 ggcaacgcgc gggcgccgtc gtcgacggac agccgcaacc gtttaccctc gatccgcgac
      961 tcccggacgg acggcagcgc ggccaggtcc cgttgcagca cttcgagcgc gatacgctcg
     1021 ccatgcggat tgatcgtgat ggcctcaccg gcgagacgct gcttcagttc ctgtggcgta
     1081 ccctcggcga cgatccggcc gtggtcgatg atcgccagcc ggtccgagag tgcgtcggcc
     1141 tcctccagat agtgcgtcgt caggaagaca gtcgtgccgc catcgcgcag ctggtgcacc
     1201 tggccccaga ggttggcgcg gttctgcggg tcgagccccg tcgtcggctc gtcgaggaag
     1261 aggacggccg gctggtggac gataccaagc gcgacatcca gccggcgacg ctggccgccg
     1321 gagtaggagc gcacggcccg atcagcgaac ggctccagct cgaagaagcg gatcagctca
     1381 tccgcgcgcc gtgcggcaac gggtgtcgct gctccgtaga gctgcccttg caggatcaga
     1441 ttctcgcggg ccgtcgccgt cggatcgctg ccgccggcct ggctgacgta gccgatgcac
     1501 cggcgcacgt cctcaggctg gcgccgaaca tcgtagccgc cgatcctggc ctcgccgctg
     1561 tcgatcggca gcagcgtcgt caacatccgt aggacggtcg tcttgccggc cccgttcggg
     1621 ccgagaaagc cgaagatctc gccccggctg acctccaggt cgacaccttg tactgccgcg
     1681 accatcacac cgttgacctg gaacgacttc cgcagatcgt gggtgaacac tgcctgttgg
     1741 gtctccatct gccatcaact ccgcttctaa ctgccaccgg ctcaatccgg ttcgaccaaa
     1801 gtatcatcct ttcggatgat actgtcaacc atacagatga ctgataagat catggcgacg
     1861 aagatcgatc tgcacagcga accatgagga ggctcgacgc cgatgatcgc ccgtgatgat
     1921 gagcgtcagg aggcgcttgg ccgtcgcacc cccgcgcggg atatgcggca acgcgatgcc
     1981 gagcggtcgc gggagctgct gctgagcgcc gcgctcgacg agtttgcggc gaaggggttc
     2041 gccggcgccc gcgtccagga catcgcctcc cgcgctggcc tgaacaagca actgattacc
     2101 tactacttcg gcggcaagga ggggctctac caggcgctcg aacggcgctg gctggatctc
     2161 gaatcgacct tcgcacggcc cgatatcccg atcgacgagc tggccgtcga gtacctccac
     2221 gccgtcctgg ccgatccgcg cctgttccgc ctgctggtct ggaacgggct ggccgagcgt
     2281 gggggcccat ccgaccctgc cgatgaggac ccggtggggc acgacgacgt ggcggacctc
     2341 ctgcgacggc agcaggccgg cgagctggcg agcgacctcg atcccggcct cgttctgctc
     2401 gccctgatgg gtgcgattgc ggcaccgatc gtcctgcccc aggtcgtccg gagggtgacc
     2461 gggctcgatt tccgctcgcc cgagttcgag caggcctacg ccgagcagtt gcgccggatc
     2521 gtccagcatc tggccgaata ggcggggcag ccgacaaatc tgggggaaac gcgctaggat
     2581 accagttaca acacgggaga ggcgacagcg cgccggactg acagtcagtg cgccgtcgct
     2641 ctccgcacga aagagctgct ggtacctggt ctgtggtgcc accgagtcgt acgagacagg
     2701 ggacgagaga cgtgcaagaa acgatcgtaa atccgtttgc cgggctggac ctggcgcagc
     2761 tccacacgcg cacctgcgcc aagtggagcg tctatcccga cgatgtcctg ccgctctggg
     2821 tggccgagat ggacgccgtc atcgcccagc cgatcgccga cgccgtgatg gccgcactgg
     2881 ccaacggcga caccggctac ccgtatggcc gcgcctaccc ggaggcgttg gcctccttcg
     2941 ccagcgaccg ctggggctgg tcgttcgaca cggcatcaac cgcgatggtg gcggatgtca
     3001 tgagcggcgt ccgggaggtc tcgcgcgcga tcggcgccgc cgacgcgccg gtcgtcgtca
     3061 cgccgccggt ctatccaccc ttcttcgggg tcgccaagac gctgagacgg ccggtcgtcg
     3121 aggcgccgct cgatgcgcag ggccggctcg acaccgccgc cctcgaagcc gccttcaaag
     3181 aggcgacggc cggtggccgc aaggcgatca tgctgctgtg taacccgcac aacccgaccg
     3241 gaaccgtcca cagccgcagc gagctggaga tggtcgcgcg cctggcccgg cagtacggcg
     3301 tgcgggtcgt ctcggacgag atccactccc cactgatcat gccgacggca accttcacac
     3361 cctacctgag tgtggaggga accggatccg acttcgccgt cgtctcggcc tcgaagggct
     3421 ggaatctggc cggcttcaag gcggccgtcg tcgtcgccgg acccgacgcg accgacgagc
     3481 tcaagtcgtt cgggttccac ggcgccggtc acatcgccgt catcgcccac acggccgcct
     3541 tcaaccaggc gcgcgactgg ctcgacggcg cgatcgccgg gatcgacgcc aaccggaagc
     3601 tgctggccga cctgctggag acacacctgc catccaccgt ttaccagatg ccggaagcga
     3661 cctacctcgc ctggatcgac tgccgtgacc ttggcctcgg cgacgatccg accgctgcct
     3721 tcctggagcg gagcaaggtc gcgctctcgt ccggaacccc gttcggcagc cagggcgtcg
     3781 gcttcgtccg ccttaacctg gcaacctcgc cggagatcct gaccgaggcc gtgacccgaa
     3841 tggccgacgt gcgcggctga ctctgatcga cgcacacagg ccggggctgg cgggaagcat
     3901 ctcgccagcc ccgttctatg tctttcggat tgggttatgg ggcattggat tgtgctgatc
     3961 ggcgcgcgac gttggatcat cgagttgaag cgatcctgac cttctccc
//
LOCUS       DASUGJ010000589         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1346282, whole genome shotgun sequence.
ACCESSION   DASUGJ010000589 DASUGJ010000000
VERSION     DASUGJ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346282"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..854
                     /gene="nth"
                     /locus_tag="VFV93_13370"
     CDS             192..854
                     /gene="nth"
                     /locus_tag="VFV93_13370"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5166385.1"
                     /translation="MLRRLRAEYGEPERPGTIEPVDELVMTILSQHTSDQNTERAFAS
                     LRATFPTWEAVIAASDDAVADAIRSGGLANVKAPRIRKALADVRERTGGFDLSFLADL
                     SVADARGWLTSLDGVGPKTASCVLLFSLDRPAMPVDTHVHRVSLRLGLIPARTSAERA
                     HQMLEAIVPDGDMYAAHLLLIRHGRATCYARWPRCRSCILVQCCPAAQTFLSDEQEQH
                     DG"
     gene            844..1506
                     /locus_tag="VFV93_13375"
     CDS             844..1506
                     /locus_tag="VFV93_13375"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5166386.1"
                     /translation="MTVRHTEVFMVRHGQTDSNVGGLLHGATDVPLNPTGLRQADLVA
                     SRIEQLEQLHSLHSSPLRRALRTARAISSRIGLEPHLHSGLAEMNFGQVEGLTIAAMS
                     EQFPELAKRFSDFSDLDARFPGGESRREFHDRVRLALDRIVTRNSGQRLVVVAHGGVI
                     ASLIAQMLGDDPNDWRRYTIDNCSVTHLEFATSGPIAHLLNDVVHLEQIDIDELSSEA
                     GN"
     gene            1506..2288
                     /locus_tag="VFV93_13380"
     CDS             1506..2288
                     /locus_tag="VFV93_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5166387.1"
                     /translation="MPLSVTVLGGSAAWPNPGQGCSAYLVTDGVTTVLLDCGPNTLLE
                     LRKHVDYATLDAIVISHCHADHILDLVPYRYGLIYGPVDVRRQIPIWLPPGGHKRLEM
                     LGGAFAGQGEPQASFWELAFDLAEYDPDRGLEVNGLRLTFAPTQHFIPCFAMRVESSD
                     GRTLLYSSDTGSIKPILGLANGAEVVIAEATLDQVSDHPEEQRGHMTPAEAGRLAAAG
                     GAHTLIVTHLWSERPDDVVTQTAAEQFNGRILIAKPGMRVDA"
     gene            2281..>3122
                     /locus_tag="VFV93_13385"
     CDS             2281..>3122
                     /locus_tag="VFV93_13385"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX5166388.1"
                     /translation="MPDQLVWFRIGHVTNEEGRTGCTVILFDKLVPATVDVRGGAPGT
                     RETALLGSGRLVGRADALLLTGGSAFGLAAADGVMRYLKEQGRGFPTGAMPVPIVPAA
                     VIFDLANGAAVWPDADDGYQAVANAEPPAGPSSGRFGAGTGATVAKLGGGSTPGGIGV
                     ASVSTGEATVTAIVVLNALGDIVDPQTGTPRASAVDPEGRGRRARELVLNANATARSG
                     ENTTIGAVLVDGAADRDTLQRCCIAAHDGLARCVWPAHTPFDGDTFFAVARQSADLSP
                     ADTLR"
BASE COUNT          545 a         1033 c         1024 g          520 t
ORIGIN      
        1 cccgcaatcg tgttgttggt ctggttcgag gtcgcaatat cacccatagc cttcgtacgt
       61 acgcttgcct gcgacgtgct aacagagaat gatacggtct ggatgtccgg atcgtcaagg
      121 ttgctgctag aatcagcgtc atgacaccag gcgagcaaac cttccaaccg gcacccccag
      181 cgatcgagtg ggtgctacgt cggctgcgcg ccgagtacgg cgaaccagag cgcccgggaa
      241 cgatcgagcc ggtcgatgaa ctggtgatga cgattctctc gcagcacacg tctgaccaga
      301 acaccgagcg tgccttcgcc tccctacgag cgaccttccc gacctgggag gccgtcatcg
      361 ccgcgagcga cgacgcggtc gcggacgcca ttcgaagcgg cgggcttgcc aacgtcaagg
      421 ctccgcgcat ccgcaaggca ctggccgacg tgcgcgagcg aaccggcggg ttcgacctgt
      481 cgtttctggc cgatctctcc gtcgcagacg cgcggggctg gctgacgagc ctggacggag
      541 ttgggccgaa gacggcgtcc tgcgtactgc tattcagcct cgaccggccg gccatgccgg
      601 ttgacacgca cgttcaccgc gtctcgctgc ggctgggatt gatcccggcc cggacgagcg
      661 ccgagcgcgc ccaccagatg ctggaggcga ttgtgccgga cggcgacatg tatgccgccc
      721 atctgctgct gatccgccat ggacgggcaa cctgctacgc gcggtggcct cggtgtcggt
      781 cctgcattct cgtacaatgt tgcccggcgg cccagacatt tctcagcgac gaacaggagc
      841 aacatgacgg ttaggcacac cgaggtgttc atggttcgcc atggccagac cgactcgaac
      901 gtcggcggat tgctgcacgg cgcaaccgat gttccgctga atccgaccgg gctgcgacag
      961 gctgatctcg tcgccagccg catcgagcag ctggagcaac tgcattcact ccattccagc
     1021 ccgctgcggc gtgccctgcg aactgccaga gcgatctcgt cacgaatcgg tctggagccg
     1081 cacctgcaca gcgggctggc ggagatgaac ttcggccagg tggaagggct gacgattgcg
     1141 gcgatgtcgg agcagttccc ggaactggcg aaacgcttct ccgacttcag cgacctcgac
     1201 gcccgctttc cgggcgggga atcgcggcgc gagtttcacg accgggtccg gctcgccctc
     1261 gaccggatcg tcacacgcaa cagcgggcag cggctggtcg tcgtcgctca cggcggggtc
     1321 atcgcgtcac tgatcgcgca gatgctgggc gacgatccga acgactggcg gcgctacacg
     1381 atcgacaatt gcagcgtgac ccatctcgag ttcgcgacat ctggtccgat tgcccacctg
     1441 ctcaacgatg tcgtccatct tgagcagatc gacatcgacg agctttcatc ggaagcagga
     1501 aactgatgcc gctcagtgtg acggttcttg gaggttcggc cgcctggccg aatccaggac
     1561 agggatgctc tgcctacctg gtcaccgacg gggtgacgac cgtcctgctc gattgcggcc
     1621 cgaacacgct gctggagctg cgcaagcacg tcgattatgc gacgctcgac gcgatcgtca
     1681 tctcccattg ccacgccgac catattcttg acctggtccc gtatcgctat gggctgatct
     1741 acggtccggt cgacgtgcgc cgacagatcc cgatctggct gccaccgggc gggcataagc
     1801 ggctggagat gctgggcggc gccttcgccg gacaggggga gccgcaagcg tcgttctggg
     1861 aattggcctt cgacctcgcc gagtatgatc ccgaccgcgg attggaagtc aacggcctgc
     1921 ggctaacgtt cgcgccgact cagcacttca tcccctgctt cgcgatgcgc gtcgagtcgt
     1981 cggacggcag gacgttgctg tactccagcg acaccggctc gatcaagccg attctgggcc
     2041 tcgcgaacgg cgccgaagtc gtcatcgccg aggccacgct ggaccaggtc agcgaccacc
     2101 ccgaagaaca acgcggccac atgacaccgg ccgaggctgg ccgactggca gccgccggtg
     2161 gagcacacac gctgatcgtg acccacctct ggagcgagcg gccggacgac gttgtgacgc
     2221 aaaccgccgc cgaacagttc aacggccgga tcctgatcgc caagccgggg atgcgtgtcg
     2281 atgcctgatc agcttgtctg gttccgaatc ggccacgtca cgaacgagga gggccgaacc
     2341 ggttgcaccg tcattctgtt cgacaaactc gtgccggcga cggttgacgt gcgtggcggt
     2401 gcaccgggga cccgggagac ggcgctgctg gggagcggcc gcctggtagg gcgggccgac
     2461 gcgctgctac tgaccggcgg cagtgcattc gggctggcgg cggccgatgg cgtcatgcgc
     2521 tatctcaagg aacagggacg gggcttcccg accggcgcca tgccggtgcc gatcgtgccg
     2581 gcagccgtga tcttcgacct cgcgaacggc gcggccgtct ggcctgacgc tgacgacggc
     2641 taccaggcag tcgcgaacgc ggaaccgcca gccgggccgt catcaggacg cttcggggcc
     2701 ggcaccggcg cgaccgtcgc gaaacttggt ggcgggtcga cacccggagg gatcggcgtt
     2761 gcgtcggttt cgaccggcga ggccaccgtc acagcgatcg tcgtgctgaa cgcgctcggc
     2821 gacatcgtcg atccccagac cgggacacca cgagcaagcg ccgttgatcc cgaaggacgc
     2881 ggccggcggg cacgcgaact ggtgctgaat gccaatgcca ccgccagatc cggagaaaac
     2941 acaaccatcg gcgctgtgct ggtcgacggc gcggccgatc gggacacgct ccaacgctgc
     3001 tgcatcgcgg cccacgacgg gctggcacgc tgcgtctggc cggcccacac gccgtttgac
     3061 ggagacacat tcttcgccgt cgcgcgccag agcgccgatc tgtcgccggc tgacaccttg
     3121 cg
//
LOCUS       DASUGJ010000590         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_236232, whole genome shotgun sequence.
ACCESSION   DASUGJ010000590 DASUGJ010000000
VERSION     DASUGJ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236232"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="VFV93_13390"
     CDS             complement(<1..1811)
                     /locus_tag="VFV93_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5166389.1"
                     /translation="MSLTAKSSEITDISQLLGDEQRLSSEQLAVLDAIQKRILWLSTN
                     IIHYANHVRPNTDGIKVGGHQASSASVVSILTALYFHSLKAGDRVSIKPHASPAWHAV
                     QYLLGQLDPKYLTTLREYKGLQSYPSRTKDPDPVDFSTGSVGLGAVAPLFAALSHRYA
                     QAHFETVTSNRFIALIGDAELDEGNIWEALIEEALSTVGNVLWIVDLNRQSLDRVIPG
                     IRATRIKQLFAESGWRVIETKYGRRLRAIFARPGGENLRQAIDDMSNEEYQTLIRLDG
                     ATLRPRLLDRTNIDAKALESAIADVPDDELPSLLANLGGHDLEDLIAAIEEGLSTSDR
                     PTVLFAYTIKGWGLPFAGDALNHSALLNPNQLDPLRESLGIPAGEEWARFAPDSPEGQ
                     CATESWERIYAGDSVPTVAVKAEAIPAHVGHNRTRETSTQEALGRLLTQLADLPQVGE
                     RIVTAAPDVSVSTNLGGWINKAGVYAASEEQIFDQSPRLLRWEPKPSGQHIELGISEM
                     NLFMWLAMFGLSHELSGQLLFPIGTVYDPFVCRGLDAFIYGVYSHSRFIVAGTPSGIS
                     LSPEGGAHQSTITPSIGMELPQVRYYEPAFAQELEWTL"
     gene            complement(1943..2926)
                     /locus_tag="VFV93_13395"
     CDS             complement(1943..2926)
                     /locus_tag="VFV93_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5166390.1"
                     /translation="MIDVTHLSKRFWVARHHRGVGGLLRNLVSREGDEVRAVDDISFA
                     VAGGEMVGYVGPNGAGKSTTIKMLTGILVPSEGQVSVAGMVPWRQRRELALRIGVVFG
                     QRSQLWWDLPLIESLELLRHVYRVPAERFARNLAHYREILDLDPFLNTPVRQLSLGQR
                     MRGDLTAALLHEPPILYLDEPTIGLDVVAKARMREVLVQVNREQGVTVLLTTHDMSDI
                     THLCTRMLIIDHGNLLYDGSVEAIRDRFGTERTLVVDLEEGEQPTEPLIVPNATEVRA
                     DGPRRWLRFQRHEISAADLIAAVTARYRISDLTLEEPDIEGIVRRIYVEGI"
BASE COUNT          495 a         1016 c         1021 g          520 t
ORIGIN      
        1 agcgtccact ccagctcctg ggcgaaggcc ggctcgtagt agcgcacctg cggcagctcc
       61 attccgatcg acggcgtgat cgtcgactgg tgcgcgccgc cctcgggcga gagagagatc
      121 cccgatggcg tcccggccac gatgaagcgc gagtgggaat agacgccata gatgaaggcg
      181 tccagcccgc ggcagacgaa cgggtcgtag acggtcccga tcgggaagag cagctggccg
      241 gatagctcgt gcgagaggcc gaacatcgcc agccacatga acaggttcat ctccgagatg
      301 cccagctcga tgtgctggcc gctcggcttc ggctcccagc gcaagagccg cgggctctga
      361 tcgaagatct gctcctcgct ggccgcgtag acaccggcct tgttgatcca accgccgagg
      421 ttggtcgaga ccgagacgtc cggcgcggct gtcacgatcc gctcgcccac ctggggcagg
      481 tcggccagtt gcgtcagcag ccggccgagc gcctcctggg tgctggtctc cctggtccgg
      541 ttgtggccga cgtgggccgg gatcgcctcg gccttgacag ccacggtcgg gacgctgtcg
      601 ccggcgtaga tccgctccca cgactcggtg gcgcactgtc cctccggcga gtcgggggcg
      661 aagcgcgccc actcctcacc ggctgggatg ccgagcgact cccgtagcgg gtcgagctgg
      721 ttgggattca gcagcgccga atggttcagc gcgtcgccgg cgaacggcag gccccaaccc
      781 ttgatcgtgt aggcgaacag caccgtcggg cggtcgctgg tggagagccc ttcctcgatc
      841 gcggcgatca ggtcttcgag atcgtgcccg ccgaggttgg ccagcaggct cggcagctcg
      901 tcgtccggca catcggcgat ggcggattca agcgccttgg cgtcgatgtt ggtgcggtcg
      961 agcagccgcg gccgcagcgt cgcaccatcg agccggatca gcgtctggta ctcctcgttg
     1021 ctcatgtcgt cgatcgcctg gcgcagattc tcgccgcccg gcctggcgaa gatcgcccgc
     1081 agccgccggc cgtatttggt ctcgatcacc cgccagcccg attcggcaaa gagctgcttg
     1141 atccgcgtcg cccggatgcc ggggatgacg cggtcgagcg actggcggtt gagatcgacg
     1201 atccagagga cgttgccaac cgtcgagagc gcctcctcga tcagcgcctc ccagatgttg
     1261 ccctcgtcca gctcggcgtc gccgatcagc gcgatgaagc ggttgctggt aacggtctcg
     1321 aagtgcgcct gtgcatagcg gtgcgagagg gcggcgaaca gcggcgcgac ggccccgagg
     1381 ccaaccgagc cggtcgagaa gtcaaccggg tccggatcct tcgtccgcga gggatacgac
     1441 tggagcccct tgtactcgcg cagcgtcgtc agatacttcg ggtcgagttg gcccagcagg
     1501 tactggacgg cgtgccaggc cggcgaggcg tgcggcttga tcgagacgcg gtcgcccgct
     1561 ttcagcgagt ggaagtagag cgccgtcagg atcgagacga ctgaggccga cgatgcctgg
     1621 tgcccgccca ccttgatccc gtcagtgttg ggacggacat ggttcgcgta gtggatgatg
     1681 ttggtcgaga gccagaggat acgcttctgg atcgcatcca ggacggcaag ctgctccgac
     1741 gagagacgct gctcatcgcc gagcagctgg ctaatatcgg taatctccga cgatttcgcc
     1801 gtcaggctca tgggggcctc cttcgggaat cgggattcgg gattcgggac ggatcacgtt
     1861 ctcgcgttct ctcattcgat tgtccgttat tccactggaa ggggcaacct gaaacagccc
     1921 ccgaatcccg aatcccgacg cctcagatcc cttcaacata gatccgccgc acgatcccct
     1981 cgatgtccgg ttcctcgagt gtcaggtcgc tgatgcggta gcgggcggtg acggcggcga
     2041 tcaggtcggc ggcgctgatc tcgtggcgct gaaagcgcag ccagcggcgc gggccgtcgg
     2101 cgcggacctc ggtggcgttc gggacgatga gcggttcggt gggttgctcg ccttcctcaa
     2161 ggtcgacgac cagcgtccgt tccgtgccga agcggtcgcg gatcgcctcg acgctgccgt
     2221 cgtagagcag gttgccgtgg tcgatgatca gcatccgcgt gcagaggtgg gtgatgtcgg
     2281 acatgtcgtg cgtcgtcagg aggaccgtca caccctgctc gcggttgacc tggaccagca
     2341 cctcacgcat ccgcgccttg gcgacgacat ccagcccgat cgtcggctcg tcgaggtaga
     2401 ggatcggcgg ctcgtgcagg agcgccgccg tcagatcgcc acgcatccgc tggccgaggc
     2461 tgagctggcg caccggcgtg ttcaggaacg ggtcgaggtc cagaatctcc cggtagtgcg
     2521 ccagattccg cgcgaagcgt tccgccggca cgcgatagac gtgccgcagt agctcgagcg
     2581 actcgatcag cggcagatcc caccagagct gcgagcgctg gccgaagacg acgccgatcc
     2641 gcagcgccag ctcgcgccgc tgccgccacg gcaccatccc ggccaccgat acctgcccct
     2701 cgctcgggac gagaatcccg gtcagcatct tgatcgtcgt gctcttgccg gccccgttcg
     2761 gcccgacgta gccgaccatc tcgccgcccg cgaccgcgaa cgagatgtca tcgacggccc
     2821 gcacctcatc tccctcgcgc gagaccaggt tacgcagcaa cccgccaacc ccacgatgat
     2881 gccgcgcgac ccagaagcgc ttgctgaggt gagtgacgtc gatcatcgag cctccgtggg
     2941 tcgggacgtg ggacgtagga cgcggggtta gattcaatcg cgtcccgcgt cccgcgtccc
     3001 gcgtcccacc tcccacgtcc cgaacgctaa ctccccgtac tccgataatg cc
//
LOCUS       DASUGJ010000591         9568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1346463, whole genome shotgun sequence.
ACCESSION   DASUGJ010000591 DASUGJ010000000
VERSION     DASUGJ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346463"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1527)
                     /locus_tag="VFV93_13400"
     CDS             complement(166..1527)
                     /locus_tag="VFV93_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX5166391.1"
                     /translation="MSTNGNQPRALLSATPESNAGTHRLSTGEEVYLPSHDELYAELT
                     LRNRGLISRTEQEVLRQATILVAGCGAVGGATVEPLLRAGAEHFILAEPGTYEYNNAN
                     RQNMRIQDIGKNKAAAFAEKMPDINPYAAFEVHTDGITEANVADLVGRADLIIDGVDV
                     TEPPALQAKFALHREAKRLRKPIIGGYDIAGAQWTPIYDYRKPGQKLLDGKISEADLP
                     GLTPMAFLSKVISPLKIPIEMIPEIERQLIGQSASMPQLGYTALQFGVLVVRLAFDIL
                     MDRPVKKNAIIDVNAEVRSTRDRISLAGKRLAMLYIVNNRMKALRREGRMGVYSPLED
                     ETFNDLKQYMQEQTWQPGEVIVRQGDPGDTFYVIVDGKVAVERGDSATDNGRKRLAEL
                     GNGDYFGEMALLSDEPRNATVTAISACTTLNLTRDAFERLLDSSRAAAKQIPKTAGDR
                     QSG"
     gene            complement(1586..3925)
                     /locus_tag="VFV93_13405"
     CDS             complement(1586..3925)
                     /locus_tag="VFV93_13405"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5166392.1"
                     /translation="MTTFYSWIARVTFRYKWWLLGLWLVIIAVAAVGAGASEHALKVG
                     GFSLPGTEFNEASNILADDLNLSSDKAALVVFHSDTLLATDKPYHDAVESAVEALKQQ
                     EHVTKVESFYETGIPDMVSPDNHTTYAWVTLEGEDNLLEEETPHLRDLVRSDTIDTYL
                     IGNAAVNYDIEKASADDLVRVERFTFPIVFILLVLVFGSLVAAGVPLVLGLGTVLISL
                     SSLWIIAQFTDVSIFALNTASMIGLGLAIDFSLIIVSRFREELTHHDVEKALDVTLQT
                     AGRSITFSGVTLALTMSVLTLFPIMVIRSIALAIVVVAITAILTGLFLLPVMLALLGH
                     RINSARVRLPFLKGNREGMWFNWAMRVMRRPWVSLAAGLTILGLLALPALWLERSGVT
                     VDALPESAESRQAFELVQRQFGLGEPTPVFIVVEAGTKGGIWQPEILEGIYDMHERLK
                     ADPRVARVQSLASLIPNPSRDWMVSLSPATIGSNSDRLRIAKRLVNIENDDTPDIPID
                     GDGTTTVIIAYPVHEDTDDETIALMKDLRANAETYAPGLANARVLVGGSVAQHYDFDR
                     VVYDQFPLLLGFSLLVTFVILMLFFHSLILPIKAILLNIVSIVAAYGVLVLVFQFGVG
                     DNLIGLTSIGAILSYTPVLLFSIVFGLSTDYEVFLLTRVREYYMQGYTNEESVAHGLE
                     KTAGIITAAGMIMIAVFGSFALTGVLVIKEIGFGLAVAVLIDTTLVRLVLVPATMKLM
                     GDRNWWMPKVLDRLVPHIDEGEISVPAPPASAPSVANGS"
     gene            complement(3968..5410)
                     /locus_tag="VFV93_13410"
     CDS             complement(3968..5410)
                     /locus_tag="VFV93_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5166393.1"
                     /translation="MASESAAALPYSPEVNAWVTDMINRAAEIRLPDLTAGHPDALYT
                     QLDRNGVTTVVLRTPALTEAQLVDLMKFRLAQYLAVNFVDPAMVYAEQIEHEPLSGVS
                     EHDIHVIAIAADSGEILCYAAVKAPPDAPAGTTLRDRERPAFPVEKVHGFGVYGRLRI
                     LPDLPIAKLREVGRFVKNQRLATLDELGARAPVEVGVALFRSLAGPLRLEVDAIVGDL
                     EEGVAKQNLDFFHAPMVVIHGTVPYEAEASYFFPRYQFCTVYPFAAFATDISQQMLAR
                     LDAIEAALELPGKAGLLTLFRLKRDVAETRSTLIPPEGLAPLTDATLPQKGVAMASRR
                     QMLDLAGQLRQTDLFKGLSVAEATVLGTFMERVSADAGEVIVRQGETGDDMYLIESGM
                     ADVRIGRGLAEGVTVATLGPGDFFGEIALLTGEERIADVVAAEPMTLLRLDRDSYDRY
                     LAHAAEVEQQMTRAAMSRTRETTRRMISGQ"
     gene            complement(5452..7080)
                     /locus_tag="VFV93_13415"
     CDS             complement(5452..7080)
                     /locus_tag="VFV93_13415"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEX5166394.1"
                     /translation="MRVTPNRGRILPLLLAVLLGSTGAFGMLAGTVAQSETTVEVYAS
                     GLINPKGMAFADDGTLYVAESGAPGDVMVPLPVNFGGEGPIGSNARVSKIPPGGERED
                     AITGLPNIGLYGGVEMLGAGSVTNMGGQLYEVAAGHMTISPELSRMNGDTLEPIADIG
                     AFNKNNPPPPTNGDAVPLGNPYDLVSSEGNLYITDGNFNRVLKVTPEGDISILAYWEN
                     SPVTVGATVGPDGSIYVSQFSPAPYTPGSARIDKIALDGTVTEGVVTNLTTPIDVAFA
                     PDGTMYVLRYAAEVSPEQKRYIAFGGEVQRVLADGSIEPVVTNMVFPSAMTFGPDGAL
                     YVTNYGNEANNGEGQVLRVVPGNTTVAGPDVPAPDETGSYTNVQPTQPPVEGVEPVGK
                     ITIVEPQDAMQWGYDPKTITIETGQALTFTNSGRIGHTATAKDGDFDTGLLQGGQSAT
                     IEFDKPGEFQFYCQPHPWMTATIIVEGEPVGNTTTTAVTRDEPDPPSISFAKAAIFAG
                     ILIALVFGAGYAMRRPNRAAEEPAAPAAPDGDDD"
     gene            7443..7754
                     /locus_tag="VFV93_13420"
     CDS             7443..7754
                     /locus_tag="VFV93_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166395.1"
                     /translation="MTTTNTTTTDATNFVTTLQNWRETLTTTQQTLLDKILTTATTTT
                     TTDTTTNDVTGYMTTGTTDTTTGTTTTTPDWTAVTNLLTTATTTTGTTGTTTTTTTTT
                     T"
     gene            8027..8776
                     /locus_tag="VFV93_13425"
     CDS             8027..8776
                     /locus_tag="VFV93_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166396.1"
                     /translation="MVTIADRPVELAVSPSPTTIIEAVNVTKTYDTGAVRVPALRSVN
                     LVVNQGEMVAIMGPSGCGKTTLLNCLSGLDEIDSGEIRIAGSDLSALPDNARTEFRAR
                     EMGFIFQFYNLLPVLSAVENVELPLLVSGVKPKEARARALDVLDQIGLADWAEHRPAE
                     LSGGQRQRVTIARALVNEPSIVWGDEPTGDLDSQNADEIVRLMRELNLRNGQTFVLVT
                     HDPRIGASCDRIIRMKDGQIDDDGLSAVPTG"
     gene            8789..>9568
                     /locus_tag="VFV93_13430"
     CDS             8789..>9568
                     /locus_tag="VFV93_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166397.1"
                     /translation="MNTIFGAPSSTILIVLLVIFAAFMSGSAMLFVRNRVIFKLGVRN
                     LPRRPSQTLLIVIGLMLSTLIISAAFTIGDTTHHSIRSEVLDVLGPADELVVLSSGSD
                     DETAAALTGGQIPEHIASELNAALGDNPDVDGIVPILGEPVPVINLRTKLSEPSLLMT
                     GVDPVAVESFGGLHDLAGNSIDLRSMSGIVLSESAAEKLDADVDDGLLIYVDNQPHQL
                     LVKAIAPDSLLTGATGGSSSAGFALPLVRVQTMLGQPSLVSY"
BASE COUNT         1671 a         3041 c         3163 g         1693 t
ORIGIN      
        1 gcgccgcggc caaacagatc ccgaagacgg cgggtgatcg ccagtcgggc tagcctaggc
       61 gtctgccaat atcacaatca caagggccaa tccggtaggg gcgatattgc gatatcgccc
      121 ctaccggatt ggccattgtg attgtgatat tggcagacgc ctaggctagc ccgactggcg
      181 atcacccgcc gtcttcggga tctgtttggc cgcggcgcgg ctgctgtcga gcaggcgttc
      241 gaaagcgtcg cgggtcaggt tgagtgtcgt gcaggccgag atcgccgtca cagtcgcgtt
      301 gcgtggctcg tcggagagta gcgccatctc gccgaagtag tcgccgttgc cgagctcggc
      361 caggcgcttg cggccgttgt ccgtggccga gtcgccccgt tcgaccgcga ctttgccgtc
      421 gacgatgacg tagaacgtgt cgccagggtc gccctggcga acgatgacct cgccgggctg
      481 ccaggtctgt tcctgcatgt actgcttgag gtcgttgaac gtctcatctt ccagcgggga
      541 gtagacgccc atccggccct cgcggcggag ggccttcatt cggttgttca cgatgtagag
      601 catcgccagc cgcttgccgg ccagcgagat gcgatcgcgc gtcgagcgga cctcggcgtt
      661 gacgtcgatg atcgcgttct tcttgaccgg gcggtccatc aggatgtcga acgcgagccg
      721 gacgacgagg acgccgaact ggagtgccgt gtagccgagc tgcggcatcg aggccgactg
      781 accgatcagt tgccgctcga tttccgggat catctcgatc ggaatcttga gcggtgagat
      841 gactttcgag aggaaggcca tcggcgtcaa ccccggcaga tccgcctcgc tgatcttgcc
      901 atccagcagc ttctggccgg gcttgcggta gtcgtagata ggagtccatt gggctccggc
      961 gatgtcgtag ccgccgatga tcggtttcct cagtcgcttc gcctcgcggt gcagcgcgaa
     1021 cttagcctgg agagccggcg gctcggtcac gtcgacgccg tcgatgatca ggtcggcgcg
     1081 accgacgagg tcggccacgt tggcctcggt gatgccgtcg gtgtggacct cgaatgcggc
     1141 gtacgggttg atatcgggca tcttctcagc gaaggcggct gccttgttct tgccgatgtc
     1201 ctggatgcgc atgttctggc ggttggcgtt gttgtactca taggtaccgg gttcggccag
     1261 gatgaagtgc tcggcgccgg cccgcagcag cggctcgacc gtcgcgccgc cgactgcgcc
     1321 gcaaccggcg acgaggatcg tcgcctgccg caacacctcc tgctcggtcc gcgagatcag
     1381 acctcggttg cgcagcgtca gctcggcgta gagctcgtcg tgcgacggca ggtagacctc
     1441 ttcgccagtc gagaggcgat gggtgcccgc gtttgactcc ggcgtggccg agagcagggc
     1501 acgcggctgg ttcccgtttg tagacattgg cgtccctccc tgttgcgggg tcactcgccc
     1561 cggaactcgt cgttggtccg aactgctagc tcccgttggc gacactcggc gcggaggccg
     1621 gcggggccgg taccgatatt tcgccctcgt cgatgtgcgg gacgagccgg tcgagcacct
     1681 tcggcatcca ccagttgcgg tcgcccatca gcttcatcgt cgccgggacg agaacgagcc
     1741 ggacgagcgt cgtgtcgatc aggacggcga cggccagccc gaagccgatc tccttgataa
     1801 ccagcacgcc ggtcagcgcg aaggagccga agacggcgat catgatcatt ccggcggccg
     1861 tgatgatgcc ggccgtcttt tccagcccgt gtgcgaccga ctcctcgttg gtgtaaccct
     1921 gcatgtagta ctcacggaca cgggtcagca ggaagacctc gtagtcggtg ctgagaccga
     1981 agacgattga gaagagcagg accggcgtgt acgagaggat cgcgccgatc gaggtcaggc
     2041 cgatcaggtt gtcgccaacg ccgaactgga agacgaggac gagaacgcca taggcggcca
     2101 cgatcgagac gatgttgagc aggatcgcct tgatcggcag gatcagcgag tggaagaaca
     2161 gcatcaggat cacgaacgtc accagcaggc tgaagccgag cagcagcggg aactggtcat
     2221 agacgacccg gtcgaagtcg tagtgctggg cgaccgagcc gccgaccagc acgcgcgcat
     2281 tggccaaccc cggcgcgtag gtctcggcgt tggcgcgcag atccttcatc aacgcgatcg
     2341 tctcgtcgtc ggtgtcctcg tggaccgggt aggcgatgat gaccgtcgtt gtgccgtctc
     2401 cgtcgatcgg gatgtccggc gtgtcgtcgt tctcgatgtt gacgagccgc ttggcgatgc
     2461 gcagccggtc gctgttcgat ccgatcgttg ccggtgagag cgagaccatc cagtcccgcg
     2521 acggattggg gatcaggctg gcgagcgact ggacccgggc gacgcgcggg tcggccttca
     2581 gccgctcgtg catgtcgtag atgccttcga ggatctccgg ctgccagata ccgcccttgg
     2641 tgccggcctc gacgacaatg aagaccggcg tcggctcgcc gaggccgaac tggcgctgga
     2701 ccagctcgaa cgcctgacgc gattcggcac tctccggcaa cgcgtcaacc gtcacgccgc
     2761 tgcgctccag ccagagggcc ggcagcgcca gcaggccgag gatggtcagc ccggccgcca
     2821 ggctgaccca cgggcggcgc atgacgcgca tcgcccagtt gaaccacatc ccctcgcggt
     2881 tgcccttcaa gaatgggagc cggacgcggg cgctgttgat gcgatggccg agcagggcca
     2941 gcatgaccgg cagcaggaag agaccggtca ggatcgctgt gatcgcgacg acgacgatcg
     3001 ccagcgcgat cgagcggatg accatgatcg ggaagagcgt caggaccgac atcgttagcg
     3061 ccagcgtcac tcccgagaac gtgatcgagc gacccgctgt ctggagcgtg acgtcgagcg
     3121 ccttctcgac atcgtggtgg gtcagctcct cacggaagcg actgacgatg atcagtgaga
     3181 agtcgatcgc caggccgagg ccgatcatcg aggcggtgtt gagcgcgaag atcgagacat
     3241 cggtgaattg ggcgatgatc cagagcgatg agagcgagat gaggaccgtc ccgaggccga
     3301 ggacgagcgg cacgccggca gcgaccagcg agccgaagac cagcacgagc aggatgaaga
     3361 cgatcgggaa cgtgaagcgt tcgacgcgga cgaggtcgtc agcactcgcc ttctcaatgt
     3421 cgtagttgac ggccgcgttg ccgatcaggt aggtgtcgat cgtgtccgag cggacgaggt
     3481 cgcgcaggtg cggcgtttcc tcttcgagca ggttgtcttc accttcgagc gtgacccagg
     3541 cataggtcgt gtggttgtcg gggctgacca tgtcggggat gccggtctcg tagaatgact
     3601 cgaccttggt gacgtgctcc tgttgcttga gcgcctcgac ggccgattca acggcgtcgt
     3661 ggtacggctt atcggtcgcc agcaacgtgt cggagtggaa gacgaccagc gcggccttgt
     3721 cggacgagag gttgaggtcg tcggcgagaa tgttggacgc ttcgttgaac tcggtgccgg
     3781 gcaggctgaa cccaccgacc ttcagcgcgt gctcagatgc accggcgccg acagcggcaa
     3841 cggcgatgat gaccagccag agacccagca gccaccactt gtatcgaaat gtcacccgcg
     3901 caatccagga gtagaacgtc gtcatgcttg cccttcgtcc cagacggtgc gtgccgcgct
     3961 ccggccgcta ctgaccggag atcattctgc gggtcgtctc acgggtgcgc gacatcgccg
     4021 cgcgggtcat ctgctgctcg acctcggcgg cgtgcgccag gtaacggtcg tagctgtcac
     4081 ggtcgagccg cagcagtgtc atcggctcgg cggccacgac atcggcgatc cgttcctcgc
     4141 cggtcagcag cgcgatctcg ccaaagaagt cgcccggccc cagtgtcgcg acggtgacgc
     4201 cctccgccag cccgcggccg atccggacat cggccatccc cgactcgatc aggtacatgt
     4261 cgtcgccggt ctcaccctgg cgcacgatca cctcgccggc atcggctgag acgcgttcca
     4321 tgaaggtgcc gagcaccgtc gcttcggcga ccgacagccc cttgaagaga tcggtctgcc
     4381 gcagctggcc ggccaggtca agcatctgcc gccggctggc catcgcgacg cccttctgcg
     4441 gcagcgtcgc gtcggtcagt ggcgcgagcc cctcgggcgg gatcagcgtg ctacgtgtct
     4501 cggcgacatc gcgcttgaga cggaagagcg tcagcagccc ggccttgccc ggcagctcca
     4561 gcgccgcctc gatcgcatcc agccgcgcca gcatctgctg cgagatgtcg gtcgcgaagg
     4621 cggcgaaggg gtagaccgtg cagaactggt agcgggggaa gaagtaggac gcctcggctt
     4681 cgtacgggac ggtcccgtgg atgacgacca tcggcgcgtg gaagaagtcc aggttctgct
     4741 tagcgacgcc ctcttcgaga tcgccgacga tcgcgtcgac ttccagccgc agcggcccgg
     4801 ccagcgagcg gaagagcgcg acgccgacct cgaccggcgc ccgcgcaccg agctcatcga
     4861 gcgtcgccag gcgctggttc ttgacgaacc gcccgacctc gcgcagcttc gcgatcggca
     4921 ggtccggcag aatccgtagc cggccgtaga cgccgaagcc gtgcaccttc tcaaccggaa
     4981 acgccggccg ttcgcggtca cgcagggtcg tcccggccgg cgcgtcgggc ggcgccttga
     5041 cggcggcgta gcagagaatc tcgcccgagt cggccgcgat ggcgatgacg tgaatgtcat
     5101 gctccgagac accggacaac ggctcgtgct cgatctgctc ggcatagacc atcgcaggat
     5161 cgacgaagtt gaccgccagg tactgcgcca gccggaactt cataaggtcg accagttgcg
     5221 cttcggtcag cgccggcgtc cggaggacga ccgtcgtcac tccattgcga tccaactgcg
     5281 tgtagagcgc atcgggatgc ccggccgtca gatccggcag gcggatctcg gctgcgcggt
     5341 tgatcatgtc ggtcacccag gcgttgactt ccgggctata tggcaaggcg gctgcggatt
     5401 cgctggccat gtcatcctcc tgcgagcggt tcagcgtcaa gccggcatcg gtcagtcgtc
     5461 gtcgccgtcg ggcgcggccg gcgcggccgg ttcctcggcg gcgcggttgg gccggcgcat
     5521 cgcgtagccg gcgccgaaga cgagcgcgat caggataccg gcgaagatcg ccgccttcgc
     5581 gaacgagatc gacggtgggt ccggctcgtc gcgggtaacg gccgtcgtcg tcgtgttgcc
     5641 gaccggctcg ccttcgacaa tgatcgtcgc cgtcatccac gggtgcggct ggcagtagaa
     5701 ctggaactcg ccgggcttgt caaactcaat cgtggccgac tggccgccct ggagcaggcc
     5761 ggtgtcgaag tcgccatcct tggcggttgc tgtatggccg atccggccgc tgttggtgaa
     5821 cgtcagcgcc tggccggtct cgatcgtgat cgtcttcggg tcgtagcccc actgcatcgc
     5881 gtcctgcggc tcgacgatcg tgatcttgcc gaccggctcg acgccctcga ccggcggctg
     5941 tgtcggctgg acattcgtgt agctgccggt ctcgtcgggc gcgggcacat ccggcccggc
     6001 gacggtcgtg ttacccggca cgacccgcag cacctgtccc tcgccgttgt tggcctcgtt
     6061 gccgtagttg gtgacataca gcgcgccgtc cgggccgaac gtcatcgccg acgggaagac
     6121 catgttggtg acgaccggct cgatcgagcc gtccgcgagg acgcgctgga cctcgccgcc
     6181 gaaggcgatg tagcgcttct gctccggtga gacctcggcc gcgtagcgca ggacgtacat
     6241 cgtgccgtcc ggcgcgaagg ccacgtcgat cggcgtcgtc aagttcgtca cgacaccttc
     6301 ggtgacggtt ccatccagcg cgatcttgtc gattcgggcg ctaccgggcg tgtacggggc
     6361 cgggctgaac tgcgagacgt agatgctgcc atccggcccg accgtcgcgc cgaccgtgac
     6421 ggggctgttc tcccagtagg ccaggatgct gatgtcaccc tccggcgtga ccttgaggac
     6481 acggttgaag ttgccgtcgg tgatatacag gttgccctct gacgagacca ggtcgtacgg
     6541 gttgccgagc gggacggcgt cgccgttggt cggcggcggc gggttgttct tattgaaggc
     6601 gccgatgtcg gcgattggct ccagcgtgtc accgttcatc cgcgacagct ccggcgagat
     6661 cgtcatgtgg ccggccgcaa cctcgtagag ctggccgccc atattggtga cgctgccggc
     6721 gccgagcatc tcaacaccgc catagagccc gatgttgggc agaccggtga tcgcgtcctc
     6781 gcgctcgcca ccgggcggga tcttcgagac gcgcgcgttg gagccgatcg gcccttcgcc
     6841 gccgaagttg accggcagcg ggaccatcac atcccccggt gcgccggatt cggcgacgta
     6901 gagggtgccg tcgtcggcga aggccatccc cttcgggttg atcaggccgg aggcgtagac
     6961 ctcgaccgtc gtttcagact gggcgaccgt gccggccagc atcccgaacg cgcccgtcga
     7021 gccgagcagg acggcaagca gcagcggcag aatgcgacca cggttgggcg ttactcgcat
     7081 gaaacacagc tttcgctgga agcagcgtta accatcagat gaggtcggta caactcctgg
     7141 aaaccgcggg tcgccgaggg gttgggccat ggatacgatg ggcggatcgt aacagatgct
     7201 tctctggcca gtcaacgaca cgcgggccag gtcgaacgct gctctaaaga gcaggaaggc
     7261 ggttcatcgt cgttgcccgt cgtttctgct gcgatggtgc atccatgttg acggattcgc
     7321 tgtatcagcc gctacagatc ggctgtaaag ttgtgcgttg accctgttct gagcgcatgc
     7381 tagtctgcaa atcacggagc agcagttcta taagatgggc ttctcgggca aaggagaatg
     7441 gcatgacgac caccaatacc acgacgaccg atgccaccaa tttcgtcacg acgctccaga
     7501 actggcgcga gacgctgacg accacccagc agacgctgct ggacaagatc ctcacgacgg
     7561 cgacgaccac gaccacgacc gacacgacaa ccaacgacgt gaccggttac atgacgaccg
     7621 gcacaaccga cacgacgacc ggcacgacca caacgacgcc ggactggacg gccgtgacca
     7681 acctgctgac gacggcgaca acgacgaccg gcacgaccgg cacgacgacc acaaccacaa
     7741 cgaccacgac ctagcgcagg gtcaccacgg ctcgtcacaa cgaagcgctg agtcggtgcc
     7801 agggcggctg ctccgcaacc ctggcaccct cagcgcttcg gcgtgtctgc cagttgaacg
     7861 acattgacac acgattggca tgcaggagat cgtcctccca acgctcgtga caccgcccgc
     7921 gtcgtaggct cgtggtggta cgaaagagcg atgcgagggc gttcgcctgc gtattcggaa
     7981 gcgtatggct ggtgccctcg tccgagagga gaggaacggc cagcgcatgg ttaccattgc
     8041 agatcggcct gttgagctgg ccgtgtcacc atcacccaca accatcatcg aagccgtcaa
     8101 cgtcaccaaa acctacgata cgggagccgt cagggtcccg gcactacgaa gcgtcaatct
     8161 cgtcgtcaac cagggcgaaa tggtcgccat catgggaccg tccggctgtg gcaagacgac
     8221 gctactcaac tgcctctcgg ggctggatga gatcgacagc ggtgagatcc ggatcgccgg
     8281 gtccgacctg tcggcgctcc ccgacaacgc gcggaccgag ttccgtgccc gcgagatggg
     8341 attcatcttc cagttctaca acctgctgcc agttctctcg gctgtcgaaa atgtcgagct
     8401 gccgctgctc gtttccggcg ttaagccaaa ggaggcgcgg gctcgtgctc ttgacgtgct
     8461 cgatcagatc ggccttgccg attgggccga acaccgtccc gccgaactct ccggcgggca
     8521 gcggcagcgg gtgacgattg ccagggcgct cgtcaacgag ccgtcgatcg tctggggtga
     8581 cgagccgact ggcgaccttg acagccagaa cgccgatgag atcgttcgcc tgatgcgcga
     8641 gctgaacctg cgtaacgggc agacattcgt cctggtcacc cacgatccac ggatcggcgc
     8701 cagctgcgac cggatcattc ggatgaagga cggccagatc gacgacgatg gtctgtccgc
     8761 cgtgccgacc ggctaggaag gttgcaccgt gaacaccatc tttggcgcac cctcatcgac
     8821 gattctgatt gtcctgctgg tcatcttcgc ggcattcatg agtggctcgg ccatgctctt
     8881 cgtgcgcaac cgggttatct tcaaactcgg tgtccgcaat ctgccgcgcc ggccatcgca
     8941 gacgctgctg atcgttatcg gattgatgct ctcgacactg atcatctctg ccgcgttcac
     9001 gatcggcgac acaacgcacc acagcatccg gtcggaggtc ctcgatgtcc tcgggccggc
     9061 cgacgagctc gtcgtcctgt cgagcggcag cgatgatgag acggctgccg cgttgacggg
     9121 gggccagatc ccggagcata tcgcgagcga actcaacgcg gccctcggcg ataacccgga
     9181 tgtggatggg atcgtgccga tcctcggcga gccggttccc gtcattaacc tgcgcacgaa
     9241 gctcagcgag ccatcgctgc tgatgaccgg tgttgatccg gtggctgtcg agtcattcgg
     9301 cggcttgcac gacctcgctg gcaactcgat cgacctccga tcaatgtccg gcatcgtcct
     9361 cagcgagtca gcggccgaga agctggacgc agacgtggac gacggactcc tcatctacgt
     9421 cgacaaccag ccgcaccagt tgctcgtcaa ggcgattgct cccgattcgc tcctcaccgg
     9481 agcaaccgga gggagctcat cggccggctt tgcgctgccg ctcgtccgag tccagacaat
     9541 gcttgggcag ccctcgctcg tgagttac
//
LOCUS       DASUGJ010000592         5210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1069148, whole genome shotgun sequence.
ACCESSION   DASUGJ010000592 DASUGJ010000000
VERSION     DASUGJ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069148"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="VFV93_13435"
     CDS             complement(<1..1494)
                     /locus_tag="VFV93_13435"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX5166398.1"
                     /translation="MLVWAGLWLVSLAGAPTVSAHATLLRSDPAAAASFTSGPDVISL
                     WFSEDVEVEYSRIEIVRRDGSRVLAGDLDRLPESPKTALRLTLADPLPDGSYTVVWST
                     LSSVDSHVSEGYFSFTVGDAILPSATEEADLARTAESDKVIPQAIDGLVRWLNLLGQA
                     AIAGLLIFLPLILMPVLSDAGERRIAIPARRYRLLLFGALAVLVAGQLGAAVIQIMNA
                     TRSTGLAVVGEPLISLLTGTRYGALWLSRSVLIVALAVLLWLLTRGRRLMSTGGQGRV
                     VWMWAGWMAALVLLTTSLGSHAAARSGAQSLPVALDWLHLLGTSVWTGGLLGLAISLP
                     LAASVSAQTRVQLLRRFSLFALAAFAVLAVTGTIAALREVSSWDGLTATRYGLWFTIK
                     LVVVAGAVGFGAWHWLVAGPALASGPETIASRAARGLRQTVRAEAGLLVLAVAATGLL
                     TSSIPARDLLDAGPPTFGSTRLTSLASITLRATPGQIGSNEFSVVVGP"
     gene            1951..3123
                     /locus_tag="VFV93_13440"
     CDS             1951..3123
                     /locus_tag="VFV93_13440"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HEX5166399.1"
                     /translation="MSEHDGVIELTRQLVAARSDAETGEQAAIDVARAAMVDLGFQDV
                     TIDPLGNLTGSVPGSGGTGCLVFDGHADTVGVGDASNWASDPYAMEQRGTRLYGRGVS
                     DMKGSIAAMIYGVARLKDDPIACDVVVSVSIAEELVEGHALGYVLDRYQPKAVVIGES
                     TSMALARAQRGRAEVLVETFGVPAHSSTPQLGRNAIKPMAALVNLLSSLPMPSDALLG
                     PAILEVTDIISHPYPALSVIPEHCMATFDRRMLVGETDEDVLAEVQAILDAYMQDDPS
                     LDARASIAVDRFDTYTGYQVEAPNCAPAWRMADGAPIVQAGISALTQAGLLPILSHYA
                     FCTNGSESAGRRNIPTVGFGPGDEAEAHRIDESIEVAHLLASVTGYEMLAREMAKL"
     gene            3123..3914
                     /locus_tag="VFV93_13445"
     CDS             3123..3914
                     /locus_tag="VFV93_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5166400.1"
                     /translation="MGGVRESGFGNWEAESSQGGEGHASGDTSRIPNPESRPTTDPQD
                     ELFDVLDDEGRPTGVAKRRGDVHRDGDWHGALHIWVCGVGDDGRSFTLFQRRSMTKDT
                     WPGYLDTTIGGHIRSGETLAETVREAEEEIGLAVSLDDLTWIGRRSVGGSRGQIIDRE
                     VQSIYAVRSDQPLPDYRLHPQEVDALVAVELDAVRRLFAAETESVPARECPRGGQPFE
                     ITITRDDFAIRDRTYVLAVLPGLAVVIAGRTPEPFDMRGQLPNPM"
     gene            complement(4034..>5210)
                     /locus_tag="VFV93_13450"
     CDS             complement(4034..>5210)
                     /locus_tag="VFV93_13450"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5166401.1"
                     /translation="FNNPTVYAEKYFDHPRHIEIQIMADTHGNVVGLGERDCSIQRRH
                     QKLVEESPSPAIDAEMRAAMNATAEALARSVDYVNAGTLEFLVQDGQFYFLEMNTRIQ
                     VEHPVTELVTGIDLVREQLRVAAGQPLSFSAPPQPWGHAIECRVNAEDPAIDFKPTPG
                     PLLRYVPPAGFGVRVDSGFVEGGAIDPRFDSLIAKLIVWGRDRPEALSRLDRALADFE
                     VAGVATTIPLFRALLRHPDFAAGSYDTRFLERTELASLVPPFTPPAAAEPEEGIVVVE
                     VDGRPYRVKLPDGIGATGGRSSHSAPRRETRRPSKTVAPTGNTLTSPIQGTVLSVAVA
                     VGDTVETGQLICVVEAMKMENEMVAHQAGTVTELHVEPGKTVQTGGALAVIEPAQSM"
BASE COUNT          859 a         1718 c         1861 g          772 t
ORIGIN      
        1 cggcccgacg acgaccgaga actcgttgct accgatctgg cccggtgtcg cgcgcagtgt
       61 gatcgacgcc agcgacgtca gtcgggtcga gccgaaggtt ggcggacccg catcgagcag
      121 gtcgcgggcg gggatgctgg aggtcagcag cccggtcgcc gcgacggcca ggacgagcag
      181 cccggcctcg gcccgcacgg tctggcgcag ccctcgggct gcccggcttg caatcgtttc
      241 cggaccagac gccagcgccg gcccggcgac cagccagtgc caggcgccga agccgactgc
      301 gccggcgacg acgacgagct tgatcgtgaa ccagaggccg tagcgggtcg cggtcagccc
      361 atcccacgag gagacctcac gcagagccgc gatcgtaccg gtcacagcca gcactgcgaa
      421 ggccgccagg gcgaacagcg agaagcgccg cagcagctgc acccgcgtct gcgcgctgac
      481 ggacgccgcc agcggcagcg agattgccag cccgagcagc ccgccggtcc agaccgaggt
      541 gccgagcagg tggagccagt cgagcgcgac cggcagcgac tgcgccccgc tccgtgccgc
      601 cgcgtggctg ccgaggctgg tcgtcagcag gacaagcgcc gccatccagc cggcccacat
      661 ccagacgacc cgcccctgac cgcccgtcga catcagccgc cggccacgcg tcagcagcca
      721 gagcagaacg gccagcgcga cgatcaggac cgaacgcgac agccagagcg ctccataacg
      781 ggtgccggtc agcagcgaga tcagcggctc gccgacgacg gccagcccgg tcgagcgggt
      841 ggcattcatg atctggatga cggccgcgcc aagctgcccg gcaacgagga ccgccagcgc
      901 cccgaacagc agcagccggt agcggcgggc gggtatcgcg atccgccgct cgccggcgtc
      961 gctcaggacc ggcatcagga tcagcggcag gaagatcagc agcccggcga tggctgcctg
     1021 cccgagcagg ttcagccagc ggaccagccc gtcgatcgcc tgtgggatga ccttgtcgct
     1081 ctcggccgtc cgtgccagat cggcttcttc ggtcgcgctc ggcaggatgg cgtcgccgac
     1141 cgtgaaggag aagtagcctt cggagacgtg gctgtcaacg gaggagagcg tcgaccagac
     1201 gaccgtgtag ctgccgtcgg gaagcgggtc ggccagcgtc agccgcagcg ccgtcttcgg
     1261 actctccggt aggcggtcga gatcaccggc cagcacccgc gaaccgtcac gacggacgat
     1321 ctcaattcgg ctgtattcga cctcgacatc ttcactgaac cagaggctga tcacatccgg
     1381 cccgctcgtg aagctggcgg cggcggccgg atcggagcgc agcagcgtcg cgtgggccga
     1441 gacggtcgga gcgccggcca gcgagaccag ccagagacca gcccagacca acaacgcgac
     1501 agacgctcgg cgtgccgcgg atcgcagagg agtcgtgctc atagggggca gtagcttccc
     1561 gcgcggatta gacggcatcc ggcaaacacg gaacccctcc cgcgccgcct ggccagtctg
     1621 agtatacgcc gggacgcggg gttcgggatc cgggacgtgg gacgcgaggg atggggcatg
     1681 ggggatgggg catgagatag gatgtcgcgt cccgcgtcac gcattacgga cgacgcatga
     1741 tccatcgccc acatcactac gtatcaacca gtgacagcgg cagccatcct gttgctgatc
     1801 aggcggttcc cactctcctt ccccttcgct gggaccccgg ttgcttcctt caggcgccgg
     1861 ggtcctggtt tgtccagccc cagccgggta gcgccgggct tgtatcatcg gtggcgggaa
     1921 cggagacgcg actcaggcag ggtgggaacg atgagcgagc acgatggcgt gattgagctg
     1981 acccggcaac tggtggccgc caggagcgac gcggagaccg gcgagcaagc ggcgattgac
     2041 gtcgcgcggg ccgcgatggt cgatctgggc ttccaggacg tgaccattga tccgctcggc
     2101 aatctgaccg gcagcgtgcc cggcagcggc gggaccggct gcctcgtctt cgacggccac
     2161 gccgacacgg tcggcgtcgg cgacgcgagc aactgggcga gcgacccgta cgcgatggag
     2221 cagcgcggga cgcggctgta cggacgcggt gtttcggata tgaagggctc gatcgcggcg
     2281 atgatctacg gcgtcgcccg gctgaaggac gacccgatcg cctgtgatgt cgtcgtctcg
     2341 gtctcgatcg ccgaagagtt ggtcgagggc cacgcgctcg ggtacgtcct cgaccgctac
     2401 cagccgaagg ccgtcgtcat cggcgaatcg accagcatgg cgctggcgcg cgcccagcgc
     2461 ggccgggccg aggtgctggt cgagacgttc ggcgtcccgg cccattcatc gacgccgcaa
     2521 ctgggccgca acgcgatcaa gccgatggcg gcgctcgtca acctgctctc gagcctgccg
     2581 atgccgtccg atgccctgct cggaccggcc atcctcgaag tgaccgacat catctcacac
     2641 ccctatccgg ccctctcggt catccccgag cactgcatgg cgaccttcga tcggcggatg
     2701 ctggtcggcg agacagacga ggacgtgctg gccgaggttc aggcgatcct cgacgcgtat
     2761 atgcaggacg acccgtcgct cgatgcgcgg gcgtcgatcg ccgtcgaccg cttcgatacc
     2821 tatacgggtt accaggtcga ggcgcccaat tgcgcgccgg cctggcggat ggccgacggc
     2881 gccccgatcg tccaggccgg gatttccgcg ctgacgcagg ccgggctgct gccgatcctc
     2941 tcgcactacg cgttctgcac caacggcagc gaatcggccg gccggcgcaa catcccgacc
     3001 gtcggcttcg gtcccggcga cgaggccgag gcccatcgga tcgacgaatc gatcgaggtt
     3061 gcccacctgc tggcctccgt caccggctac gagatgctgg cgcgggagat ggcgaagctg
     3121 tgatgggcgg ggttcgggag tcgggattcg ggaactggga ggcggagtcg tcgcaaggcg
     3181 gtgaaggcca cgcgagcggc gatacctccc gaatcccgaa tcccgagtcc cgacccacga
     3241 ccgaccccca ggacgaacta ttcgacgtgc tcgatgacga ggggcggcca actggggttg
     3301 ccaagcggcg gggtgatgtt caccgggatg gtgactggca cggggcactg catatctggg
     3361 tctgtggcgt cggggatgat ggccggtcgt ttacgctctt ccagcggcgc tcgatgacga
     3421 aggacacctg gccgggctac ctcgatacca cgatcggcgg ccacattcgc tccggcgaga
     3481 cgctggcaga gacggttcgc gaggctgaag aggagatcgg cctggccgtc tcgctcgacg
     3541 acctgacgtg gatcggccgg cggtccgtcg gcggctcgcg cggccagatc atcgaccgcg
     3601 aggtgcagtc gatctacgcc gtccgcagcg accagccgct gccggattac cggctgcatc
     3661 cccaggaggt cgacgcgctc gttgcggtgg aacttgatgc ggttcgccgc ctgttcgccg
     3721 ccgagacaga gagcgtcccg gcccgcgagt gcccgcgcgg cggccaaccg ttcgaaatca
     3781 ccatcacccg cgacgacttc gcgatccgcg accgcaccta tgtgctggcg gtcctgccgg
     3841 gactggcggt cgtcatcgcg ggccgaacgc ccgagccgtt cgatatgcgg gggcaattgc
     3901 caaacccaat gtaggggcga atcggtgatt cgcccgacaa tagggccgcg ttcagcggcc
     3961 acaataggtc aattggcgca ttgggatcgg ctgaacgccg atcgaagtgt ccgggcgaat
     4021 caccgattcg cccctacatt gattgggccg gttcgatgac ggccaaagct ccgccggtct
     4081 ggacggtctt gcccggttca acgtggagtt cggtgaccgt cccggcctgg tgggcaacca
     4141 tctcgttctc catcttcatc gcttcgacga cgcagatgag ctggccggtc tcgaccgtgt
     4201 caccgacggc gacggcgacc gagaggacgg tgccctggat cgggctggtc agcgtgttgc
     4261 cggttggcgc gaccgtcttt gacgggcggc gcgtttcgcg gcggggcgcg ctgtggctgg
     4321 agcggccacc ggtcgcgccg atgccgtcgg gcagcttgac gcggtaggga cggccatcga
     4381 cttcgacaac gacgatgccc tcttccggct cggccgctgc tgggggcgtg aacggcggga
     4441 cgaggctggc cagctcggtg cgctccagga agcgggtgtc gtagctgccg gcggcgaagt
     4501 cggggtggcg caggagggcg cgaaagagcg ggatcgtcgt cgcgacgccg gcgacctcga
     4561 agtcggccag ggcgcggtcg agccgcgaga gagcttcggg ccggtcgcgg ccccagacga
     4621 tcagcttggc aatcagcgag tcgaagcgag ggtcgatcgc gccaccttcg acgaagccgg
     4681 aatcgacgcg gacgccgaag ccggccggtg ggacgtagcg cagcagcggc cccggcgtcg
     4741 gcttgaagtc gatggccggg tcctcggcgt tgacgcggca ctcgatcgcg tggccccacg
     4801 gctgcggcgg cgcgctgaac gagagcggct ggccggcggc gacgcgcagt tgctcgcgga
     4861 cgagatcgat gccggtgacc agctcggtga ccgggtgctc gacctggatg cgggtgttca
     4921 tctccaggaa gtagaactgg ccgtcctgga cgaggaactc cagcgttccg gcgttgacgt
     4981 aatcgacgga gcgggccagc gcctcggccg tcgcgttcat cgcggcgcgc atctcggcgt
     5041 cgatggcggg cgagggactc tcctcgacca gcttctggtg tcgccgctgg atcgagcaat
     5101 cgcgctcgcc gagaccgacg acgttgccgt gggtgtcggc catgatctgg atctcgatgt
     5161 ggcgcggatg gtcgaagtac ttctcggcgt agacggtcgg gttgttgaag
//
LOCUS       DASUGJ010000593         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1485639, whole genome shotgun sequence.
ACCESSION   DASUGJ010000593 DASUGJ010000000
VERSION     DASUGJ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485639"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1088
                     /locus_tag="VFV93_13455"
     CDS             258..1088
                     /locus_tag="VFV93_13455"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5166402.1"
                     /translation="MRPRSPLNPDPIELGNAWRERVEAEYEQVERLREWHEADYYAPI
                     AHHFTDDPHRTDDDLLNALRAMSDPDATWLDIGAGGGRYALPLALVSKQVIAVDPSEG
                     MIGVLRASMAEHGIENIEIHHGRWLDVAGAVEADYSLVAHVGYDIREINAFIDAVERA
                     TRRSCCVMLMDRAPSGGFTRLWQEVHGEYRYQLPGAREFLQLLLARGAMPEVRLSWRK
                     FRPMGHDDIHESARRRLWLTEGSEKDQHLQRLLNELPDGVDDFQLPSVIALMTWEPSR
                     "
     gene            complement(1116..1664)
                     /locus_tag="VFV93_13460"
     CDS             complement(1116..1664)
                     /locus_tag="VFV93_13460"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX5166403.1"
                     /translation="MCVLGCCKSSQRTEAPVHWYRALLVQFGHTRWGLFLIRTVIWRY
                     DRFLYRLTNGRVVSSGPMVFPTLLLTVTGRTTGKAHTLPLLYYRDRERLILVCADASG
                     PGSRQWPKNVLANPDVEAQIRGQTARYRARRASDDETARYWPALVEMYPPYATYAQRF
                     AETIIFVLEPSSAEKAVTESAQ"
     gene            complement(1763..>2342)
                     /locus_tag="VFV93_13465"
     CDS             complement(1763..>2342)
                     /locus_tag="VFV93_13465"
                     /inference="COORDINATES: protein motif:HMM:NF027969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166404.1"
                     /translation="KLFGQERETVEEGYAGDVVGLINPGTFAIGDTVASEPVGSFPPL
                     PRFEAEHFARLENTRTDKYKQFLRGLEGIEDEGGIQLLYPVDAHRREPILAAVGQLQF
                     DVVRARLEAEYGVETRLEPLGYEHARWVRADRETLVTLGNSRGRLRCEDRDGQQVVLF
                     STRWDLNYAVENFPTVEFSSTVWDGRRELAAR"
BASE COUNT          426 a          781 c          757 g          378 t
ORIGIN      
        1 gcgtagcacc gtcatccaga cgtcgccggt ccacatgggc accaccacca acctcgacgc
       61 cgcgctggag gatctgctcg acagcttcgt ccgcccaccg aagccgatcg aggaatgaga
      121 tactcgcgag acacgccgcg atcagcggcc atccggtagg ggcgaggcac tgactcgccc
      181 ggctccccac ccaacactct cggagtggac atgaaggacg agtcagtgcc tcgcccctac
      241 cggatggata cgtaccaatg agacctcgat ccccactgaa ccccgaccca atcgagctgg
      301 gtaacgcctg gcgcgagcgg gttgaggccg agtacgagca ggtcgagcgg ctgcgtgagt
      361 ggcacgaggc cgactactat gctccgatcg cgcaccactt cactgacgac ccgcatcgga
      421 ccgacgacga tttgctcaac gcactgaggg cgatgtccga ccccgacgcg acctggcttg
      481 acatcggcgc cggtggcggt cgctacgcgc tgccgctggc gctggtgagc aagcaggtga
      541 tcgccgtcga tcccagcgag gggatgatcg gcgtcctgcg cgccagcatg gccgagcacg
      601 gcatcgagaa catcgagatc catcatggcc gctggctgga tgtcgccgga gcggtcgagg
      661 ccgactactc gctggttgcc cacgtcggct acgacatccg cgagatcaac gcgttcatcg
      721 acgccgtcga gcgcgccacc cgccgcagct gctgcgtgat gctgatggat cgcgcgccat
      781 ccggcggatt cacgcgcctc tggcaagagg tgcacggtga gtaccgttac cagctccccg
      841 gcgcacggga gttcctgcaa ctgctgctgg cgcgcggcgc gatgccggag gtgcgcctct
      901 cctggcgaaa gttccgcccg atgggccacg acgacatcca tgagagcgcc cgccgccgcc
      961 tctggctgac cgagggatcc gagaaagatc agcatctcca gcgcctgctc aatgaactgc
     1021 ccgatggcgt cgatgacttc cagcttccga gcgtcatcgc gctgatgacc tgggagccgt
     1081 cgcgataggg cgagatgcct tgcgttgctg tcgacctact gggccgattc ggtgaccgcc
     1141 ttctccgcag aggatggttc caggacgaag atgatcgtct ccgcgaagcg ctgcgcgtag
     1201 gtggcgtacg gcggatacat ctcgacgagc gccggccagt agcgcgccgt ctcgtcgtcg
     1261 ctggcccgtc gtgcccgata tcgcgccgtt tggccgcgaa tctgcgcctc aacgtccgga
     1321 ttggcgagga cgttctttgg ccactgcctg ctgccaggtc cagatgcgtc ggcgcagaca
     1381 aggatgagcc gctcccggtc gcggtagtac aacaacggca acgtgtgcgc tttgccggtc
     1441 gttcgtccgg taaccgtgag caggagtgtt gggaagacca tcgggcctga agacacgacg
     1501 cgaccgtttg tcaagcgata cagaaaccgg tcatagcgcc agatgaccgt ccggatgaga
     1561 aagagacccc atcgggtgtg tccaaactga accaggagcg cgcgatacca gtgcacaggc
     1621 gcctccgtac gttgcgatga cttgcaacag cccaagacac acatactgta caggagtgga
     1681 ccccttggga tcagcacacg gttccgaaga ccttgcagaa gcgctttcag accccgcgtg
     1741 gtgcgatcaa ggcaaacggg ggctagcgcg cggcaagctc ccgccggccg tcccagacag
     1801 tgctggagaa ctcgacggtc gggaagttct cgaccgcgta gttgaggtcc cagcgggtcg
     1861 agaagaggac gacctgctgg ccgtcgcggt cctcgcagcg cagccggcca cgcgagttgc
     1921 cgagcgtcac cagcgtctcg cggtcggccc ggacccagcg agcgtgctcg tagccgagcg
     1981 gctccagtcg cgtctcgacg ccatactcgg cctccagccg ggcgcggacg acgtcgaact
     2041 ggagctggcc gacggcggcc aggatcggct cgcgccggtg ggcatcgacc gggtagagca
     2101 gctggatacc gccctcgtcc tcaatccctt ccagcccgcg caggaactgc ttgtacttgt
     2161 cggttcgcgt gttctccagc cgcgcgaagt gctcggcctc aaagcgcggc agcggcggga
     2221 agctgccgac cggctcgctg gcgacggtat cgccgatcgc gaacgtgcca gggttgatca
     2281 gcccgacgac atcgccggcg tagccttcct cgaccgtctc acgctcctga ccgaagagct
     2341 tc
//
LOCUS       DASUGJ010000594         5146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1208549, whole genome shotgun sequence.
ACCESSION   DASUGJ010000594 DASUGJ010000000
VERSION     DASUGJ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208549"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFV93_13470"
     CDS             complement(<1..402)
                     /locus_tag="VFV93_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX5166405.1"
                     /translation="MLPTVSTFSIVARDPANGDLGVAVASKFLAVGAVVPWLRAGAGA
                     IATQAHANVAYGPDGLAMIESGLDAQETLDRLVAADSERDLRQAGIVDSHGNAATWTG
                     PGCSDWAGGRTGDGFCCQGNILTSPEVVDAMV"
     gene            543..1310
                     /locus_tag="VFV93_13475"
     CDS             543..1310
                     /locus_tag="VFV93_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166406.1"
                     /translation="MIREPAESTGRGGRLKGRDAIVPRYGSERSFMLRTLYSWLTMSM
                     LYLFAWPLAVIYLDAPGVSPQDTPLDWYQIRLLAPVLIAVVGAWLWQLSPAPAGTIAR
                     GRALALFRQGRIWPQAVLFLLGTPLVLTAFLLAEDPAGGTKLALLTLAEALAIQILTS
                     GYLHAAFEIVLEDGRANLAATGLYAVAFAIRAAMVVASEQSDGGPYTLAIAAGIVVGL
                     IVGGISSWLRARSGSLLPGILALWLTFLALGLGDFYE"
     gene            1383..2159
                     /locus_tag="VFV93_13480"
     CDS             1383..2159
                     /locus_tag="VFV93_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5166407.1"
                     /translation="MIADDERDNTTRAYAADWRDWEGWCGRRGFRSIPASPEAIALYL
                     RGLDNAGRRYSTIRRRLAAINQRHRAAKLASPTSDALVHDVMDDIREMQDLAQIGKTP
                     LLTHDLRRMLAVLPASVSGIRDRALLLVGFAGGLRRSELVALDIDDIERQPDEMLVRL
                     PAGRGRSPRETVVELPRQPEPATCAVAATEAWLDAGSLSDGPLFRSINRHGQIGERRL
                     SDKSVALIVKRVAEDAGLDPARYAASSLRSGAAIERRLAG"
     gene            2159..3409
                     /locus_tag="VFV93_13485"
     CDS             2159..3409
                     /locus_tag="VFV93_13485"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166408.1"
                     /translation="MGAGGHGLWRNRAFRRLWASETISQTGTRVSMLALPLVAALVLD
                     ASPLQMGILAGLVNLPALVVGLFAGVFVDRWRRRWVLVVADLGRALLLGLVPVAWALD
                     LLRIELLFAVTLLTGTFSVFYAVAYRSYLPELVPREQLVEGNSKLEISRSAAEIGGPG
                     LAGVLVQLIGAPLAVITDAISFLASAALIGSIRSTERDREPVERRAGLLREAAEGLRL
                     VLGDQLLRSIAGAAATATIFSTMLEAVFILYLTRALDLRPVTIGIVFAAGSVGFLVGA
                     LLPERLTRRFGLGPTLAASLALVGLGDLLISLATGPRLVVIGVLIVAEFIFGIGLTVF
                     SINQVSLRQAVTPERLQGRMNATMLVLVSGATPLAALLGGIAGELLGLRATLLLAAIG
                     ELAAVGWIALSPTRQLRTHPESIA"
     gene            3594..4292
                     /locus_tag="VFV93_13490"
     CDS             3594..4292
                     /locus_tag="VFV93_13490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5166409.1"
                     /translation="MRPIFVLIHSPLVGPATWEPVRIELRRRGVQAAVPALRDEVAGG
                     VPYWQQAVASVMVSLRGAPAGQPLVLVGHSGAGALLPALGEALGRPVAACVFVDAGIH
                     RDGLSRLEMMDAEDAPFARQLRAHLESGGRYPEWTDAQLQEAIPDDERRAAVLADIRP
                     RGLDFFSEPIPVPAGWPDGPCRYLQLSSAYDVPARQARRLGWPVREIEGGHFLMLIDP
                     ETLAEAILALAETS"
     gene            4289..5143
                     /locus_tag="VFV93_13495"
     CDS             4289..5143
                     /locus_tag="VFV93_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5166410.1"
                     /translation="MSDPDPRAMLDALGYADATTITPVAGGRDTVIYRVEGGGSAFAL
                     RIFRPEQLRTSEREVLAMQAAAAAGLPVPRVHAAGVYEGRPVLLLDWCEGMMVVEALG
                     RWPERATALGIACGETLAAIHAVPAPAGMPAHGWLAWSGLAPDDPLYQRLAASARPDR
                     LLHLDYHPLNILTDGQWLTAVLDWTNANAGDPRADLARAIAILRLDAGDLHPGARPIL
                     RAFERGLRAGYERIAGPQANMPLFHIWAGRAMLHDLAPRLAEDPTQAARIRRWIHLWE
                     LRDRAKTD"
BASE COUNT          703 a         1722 c         1801 g          920 t
ORIGIN      
        1 gaccatcgca tcaacgacct ccgggctggt caggatgtta ccctgacagc agaagccgtc
       61 gccggtccgg cctcccgccc agtcgctgca gcccggtccg gtccaggtcg cggcgttgcc
      121 gtggctgtcg acgatgccgg cctggcgcag atcgcgctcg ctgtcggccg cgaccagccg
      181 atcgagcgtc tcctgcgcat ccagtccgga ctcgatcatc gccagcccat cgggcccgta
      241 ggcgacgttg gcgtgcgcct gggtcgcgat cgcacctgcc ccggcccgca accagggcac
      301 gactgcacca accgccagaa acttcgaggc gacggcgacg cccagatcgc cgttcgctgg
      361 atcacgcgcg acgatcgaga aggtgctgac ggtggggagc atggtcggtg tcctttcgag
      421 tgctgagtgc tgagtgctag gtccggcctc cgctaaggca cgtgacaccc cagtggcgat
      481 cgcggtcatc atactcagca ctcagcaccc aacactcagc actcgcgttg tatcctcacg
      541 ccatgatacg ggaaccggcg gaatcaacgg gacgtggtgg gcggctgaag gggcgggacg
      601 cgatcgttcc ccgctacggc agcgagcgct cgttcatgct gcgaacgctc tactcctggc
      661 tgacgatgtc gatgctgtac ctgttcgcct ggccgttggc ggtgatctac ctcgacgcgc
      721 cgggtgtctc gccgcaggat acgccgctcg actggtatca gattcggctg ctggcgccgg
      781 tcctgatcgc tgtcgtcggt gcctggctct ggcaactctc gccggccccg gccgggacga
      841 tcgcgcgcgg gcgggcgctg gcgctgtttc gtcagggacg gatctggccg caggcggtac
      901 tcttcctgct cggaacgccg cttgtcctga ccgcgttcct gctggccgag gacccggccg
      961 gcgggaccaa gctggcgctg ctgacgctgg ccgaggcgct ggcgatccag atcctgacct
     1021 ccggttacct gcacgccgcc ttcgagatcg tccttgagga cggccgcgcc aacctggccg
     1081 ccaccggcct ctacgccgtc gccttcgcga tccgggccgc gatggtggtt gcctccgagc
     1141 aatcggacgg cgggccgtac acgctggcga tcgcagccgg gatcgtcgtc ggcctgatcg
     1201 tgggcggcat ctccagctgg ctgcgcgccc gctccggcag cctgctgccc ggtatcctcg
     1261 ccctctggct gaccttcctc gccctcggcc tcggcgattt ctacgagtag cgagcccgtt
     1321 gcactcggca tcgtatcggt gtattctaac ttccgataac agcattagcg gaagttaggc
     1381 cgttgatcgc agatgacgag cgcgacaaca ccacccgagc gtacgccgcc gactggcgcg
     1441 actgggaagg ctggtgcggg cggcggggat tccgttcgat tccggcctcg ccggaggcca
     1501 tcgcgctcta cctgcgcggg ctggacaacg ccgggcgacg ttacagcaca atccgtcgtc
     1561 ggctggcggc catcaaccag cgtcaccgcg ctgccaagtt ggcttcgccg actagcgacg
     1621 cgctcgtcca tgacgtaatg gacgatattc gcgagatgca ggatctggcg cagattggca
     1681 agacgccgct gctgacccat gatttgcgtc ggatgctggc ggtactgcct gcctccgtct
     1741 ccggtattcg tgaccgggcg ctgctgctgg tcgggtttgc cggtgggctg cgtcggtcgg
     1801 aattggtcgc gcttgacatc gatgacatcg agcggcagcc agacgagatg ctcgtgcggc
     1861 tgccggctgg aaggggtaga tccccgcgcg agacggttgt cgaactgccg cgacagccgg
     1921 agccggcgac atgcgccgtc gccgcgactg aggcatggct ggatgcgggt tcgctgtcgg
     1981 atggtcctct cttccggtcg atcaacaggc acgggcagat cggcgagcga cggctgtcgg
     2041 acaagtcggt cgcgctgatc gtcaagcgcg tggccgaaga tgccggtctt gacccggcac
     2101 ggtacgccgc cagcagcctg cgatccggcg ccgcgatcga gcgccggctg gctggttgat
     2161 gggagccggc ggccacgggc tctggcgcaa ccgcgccttt cgccgtctct gggccagcga
     2221 gacgatttca cagaccggca cgcgggtaag catgctggcg ctaccactgg tggcggcgct
     2281 cgtgctcgac gcgtcaccgc tccagatggg cattctcgcg gggctggtca acctgccggc
     2341 gctggtcgtc ggcctgttcg ccggtgtgtt cgtcgatcgc tggcgtcgac gctgggtgct
     2401 ggtcgttgct gatctggggc gggcgctgct gctggggctg gtgccggtcg cctgggcact
     2461 cgatctgctg cggatcgaac tgctcttcgc ggtcacgctg ctgaccggga cgttctcggt
     2521 tttctacgcc gtggcctatc gctcctacct gccggaactc gttcctcgcg agcagctcgt
     2581 cgagggtaac agcaagctgg agataagccg ctcggctgcc gagatcggag ggccgggtct
     2641 ggcgggggtt ctggttcagt tgatcggcgc gccgctggcg gtcatcactg acgccatctc
     2701 attccttgca tcggcggcgc tgatcggctc gattcgctcg accgagcgcg atcgcgagcc
     2761 ggtcgagcgg cgcgccggtc tgctgcgtga ggcggccgag gggctgcgac tggtgctggg
     2821 cgaccagttg ctgcgctcga tcgccggcgc tgccgcgacg gccacgatct tcagcaccat
     2881 gctcgaagcc gtcttcatcc tgtatttgac gcgggcgctc gatcttcggc cagtgacgat
     2941 cggcatcgtc ttcgcggccg gcagtgtcgg attcctggtt ggcgcgctcc tgccggagcg
     3001 gctgacgcgg cgcttcgggc ttgggccgac tctggctgcg tccctggcgc tcgtcgggct
     3061 gggcgacctg ctgatctcgc tggcaacagg gccccgcctg gtcgtcatcg gcgtgctgat
     3121 cgtcgcggag ttcatcttcg ggatcgggtt gaccgtcttc agcatcaacc aggtcagcct
     3181 gcgacaggcc gttacaccgg agcggttgca gggacggatg aacgcgacga tgctggtcct
     3241 ggtcagcggc gcgacgccac tggcggccct gctcggcggt atcgccggtg agctcctcgg
     3301 cctgcgggcg acactgctgc tggccgcgat cggggaactt gctgccgtcg gctggatcgc
     3361 cctctcgccg acccgccagc tccgcacaca tccggagtcg atcgcatgaa ccggtcaaat
     3421 ggtctgaggt tggttcagtg accggtcgtt gcacgaacaa tgcgatctca aactcatgac
     3481 tttcgtaccc attccggatt ccggatatgc ggtagagtct atcgtcttac gatggattgc
     3541 tgctgtccgt tctgcgaagt gtgatatcgc gtatctcgaa aatgacagcc atgatgcggc
     3601 cgatcttcgt cctgattcac agcccgctgg tggggccggc gacctgggag ccggtgcgga
     3661 tcgagttgcg gcggcggggt gtgcaggcgg ctgtgccggc gctgcgcgac gaggtcgcgg
     3721 gcggggttcc gtactggcag caggcggtcg cttcggttat ggtgtcgctg cggggcgcgc
     3781 cggccggtca gccgctggtg ctggtcgggc atagtggggc cggggcgctg ctgccggcgc
     3841 tcggcgaggc gttggggcgg ccagtcgccg cctgtgtctt cgtcgacgcg gggattcacc
     3901 gcgacgggct gagccgcttg gagatgatgg atgccgagga cgcgccgttc gcgcgacagc
     3961 tacgcgcgca cctggaatcg ggcggtcgct accccgaatg gaccgacgcg caattgcagg
     4021 aggcaatccc cgatgatgag cggcgcgctg ccgtcctggc tgacatccgc ccacgcgggc
     4081 tcgacttctt cagcgagccg atcccggtgc cggccggctg gcccgacggg ccgtgtcgct
     4141 acctgcagct cagttcggcc tacgacgtgc cggcgaggca ggcgcgtcgg ctgggctggc
     4201 cggtgcggga gatcgaaggc gggcacttcc tgatgttgat cgacccggag acgctcgccg
     4261 aagcgatcct cgcgttggca gaaacgtcgt gagcgaccct gatccccgcg cgatgctcga
     4321 cgcgctcggc tacgcagatg cgacaacgat cactccggtc gcgggtggcc gcgacacggt
     4381 catctaccgg gtggaaggtg gcggcagcgc ctttgccctg cgcatcttcc ggccggagca
     4441 actgcggacg agcgagcggg aggtgctggc gatgcaggcc gccgcggcag ccggcctgcc
     4501 ggtgccacgc gtgcatgccg ccggtgtcta cgaaggacgg ccggtcctgc tgctcgactg
     4561 gtgcgagggg atgatggtgg tcgaggcgct gggccgctgg ccggagcggg cgacggcgct
     4621 cggcatcgcc tgcggcgaga cgctggccgc gatccacgcc gtgccggctc cggctggcat
     4681 gccggcgcac ggatggctcg cctggagcgg tctcgcgccg gacgatccgc tctaccagcg
     4741 gctggccgcc agtgcccggc ccgaccggct gctgcacctg gactaccacc cgctcaatat
     4801 cctgaccgac ggccagtggc tgacggccgt cctcgactgg accaacgcca acgccggcga
     4861 cccgcgtgcc gacctggccc gtgcgatcgc gatcctccgc ctcgatgccg gcgacctgca
     4921 ccccggcgcg cggccgatcc tgcgcgcctt cgagcgcggc ctgcgtgccg gctacgagcg
     4981 gatcgccggc ccacaggcca acatgcccct cttccacatc tgggccggcc gcgccatgct
     5041 ccacgacctc gccccccggc tggccgaaga cccaacccag gccgcacgga tcaggcgctg
     5101 gattcacctg tgggaactgc gcgatcgcgc taagacggac tgagcc
//
LOCUS       DASUGJ010000595         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1069606, whole genome shotgun sequence.
ACCESSION   DASUGJ010000595 DASUGJ010000000
VERSION     DASUGJ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069606"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFV93_13500"
     CDS             <1..229
                     /locus_tag="VFV93_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5166411.1"
                     /translation="ANVTVRAQDIDGNNLKIKARGILARVLQHEIDHLDGILYFDRME
                     DFSTLRYPTRTQAGDEDEPSTEPATAASRAG"
     gene            complement(204..875)
                     /locus_tag="VFV93_13505"
     CDS             complement(204..875)
                     /locus_tag="VFV93_13505"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5166412.1"
                     /translation="MADVPLEETTEELTRHSDALIAEIRAVARPRLFVISGPSGVGKD
                     TVIDELRGRLPEFHFAVTATTRERRPGEMHGVHYFFMSVEDFHAELASGEFVESAEVY
                     GNWYGVPRRGIQGGLDSGRHVIVKVDVQGAATIRALAPDAISIFLMPTSMEDLTRRLW
                     HRKTDGPQVVMRRVRTAAEELKAVVDFDYAVFNEEGKLSKTLDEIMAIIIAEQRKVTQ
                     RETLL"
     gene            986..2800
                     /locus_tag="VFV93_13510"
     CDS             986..2800
                     /locus_tag="VFV93_13510"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HEX5166413.1"
                     /translation="MYDVLTIAAIVDAFNSDLVHGRIQQVAQVDELTIALEVYAAHQR
                     RWLVLSAHPEHARVLLASRRVDGDAERVTPLLLLLRKYARGGRIVSVSQPRYERIVRF
                     SIAKPFRPNNSDDDEDDDGDDNIELVTTDLYVELMGRRSNIILTDDSRRILDAVKRVS
                     PEMSRVRPIRPGAAYVPPPPQDKQDPLRATPHGLLDAAGESREPLAKWLVATFRGMSP
                     ALAAETAVRAGIEPDTRAAQLSGDDAARLVSALTAMFGGIQTGEWQPMLYSWDTGRAD
                     FFAIEMQSLANEAGVTATVYPSIVEAAEAAWEANLTAVSASAGRHTVRRERLVAEIDE
                     ARARVRQRTHSLEEQVARAAEVEVLRTKGEMIYAWIGEIRQGQSNYTTPDGLTIELDP
                     TLSPSQNAQDYFERYRKAQSAEANLPALLDEAARELAYLDQLRSMAALAETYDEIESV
                     RLEWLAWAETARGGARASRPKGARPSKQARRPRAYRTSRGDAIYIGRTGQQNDEVTFS
                     IANPDDLWLHVRNMPGAHVILRANGQVSDETIERAASLAAWYSDGRNATAVPVDVTER
                     RHVRKIKGAGPGMVTYRNERTLNVRPLSEKELGLEGTG"
     gene            complement(2903..>3455)
                     /gene="tatC"
                     /locus_tag="VFV93_13515"
     CDS             complement(2903..>3455)
                     /gene="tatC"
                     /locus_tag="VFV93_13515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX5166414.1"
                     /translation="GPTEGFVTWFKVVFYIAIALAMPVLIYQLMAFLVPGLTRRERRY
                     VIRAVPFVFIMFCAGAAFAFFVLAPRMLQWLSTFGIDYFRWDPRASEIVQFYMTLMLG
                     MGLIFELPVVMYALALIGIMSAKRYGRFRKYALILGMIAAAIITPTPDPFNMMLVAIP
                     TYALYEIGILLSRTVRKSLRSQE"
BASE COUNT          677 a         1156 c         1081 g          541 t
ORIGIN      
        1 cgcgaacgtc accgtccgcg cccaggatat cgacggcaac aacctcaaga tcaaggcgcg
       61 cggcattctc gcgcgggtcc tccagcacga gatcgaccat ctcgacggga ttctctattt
      121 cgaccggatg gaagactttt cgacgttgcg ctacccaacg cgaacccagg ccggcgatga
      181 ggacgagccg agcacggaac cggctacagc agcgtctcgc gctgggtaac cttgcgctgc
      241 tcggcgatga tgatcgccat aatctcatcg agcgtcttgg agagcttgcc ctcttcgttg
      301 aagaccgcgt agtcgaagtc gacaacagcc ttcagttcct cggcagccgt tcgcacgcga
      361 cgcatgacca cctgcgggcc gtccgtcttg cgatgccaga gcctgcgcgt caggtcctcc
      421 atgctggtcg gcatcaggaa gatgctgatc gcgtccggcg ccaacgcccg gatcgtcgcc
      481 gcgccctgga catcgacctt gacgatcaca tgccggccgg agtcgagacc accctgaata
      541 ccacggcgtg ggacaccgta ccagttgccg tagacctcgg ccgactcgac gaactcgccg
      601 gaggccagct cggcatggaa gtcctcgacc gacatgaaga aatagtggac gccatgcatc
      661 tcgccgggcc ggcgttcgcg ggtcgtcgcc gtcaccgcga agtggaattc gggtagtcgc
      721 ccgcgaagct cgtcgatgac cgtgtcttta ccgacaccgg aaggcccgga gatgacgaac
      781 aggcgcgggc gggcaacagc ccgaatctcg gctatcagcg cgtctgagtg acgcgtcagc
      841 tcttctgtcg tctcttccag cggcacatcc gccatcaccc cagttacctc ccgacgcgtt
      901 ggaccctggc cagatccggc tcggtgctac aatccgctgt tgcggcgccg gaaccgtctc
      961 cggcggttct ggttggagtg ctcccgtgta cgatgtcctg acaattgccg ccatcgtcga
     1021 tgcgttcaac agcgacctcg tccacggtcg cattcaacag gtcgctcagg ttgatgagct
     1081 gaccatcgcc ctcgaagtct acgccgccca tcagcgtcgc tggctcgtcc tgagcgccca
     1141 cccggagcat gcccgcgtcc tgctcgcatc ccgtcgcgtc gatggcgacg ccgaacgggt
     1201 cacgccgctg ttgctcctgt tacgaaaata cgctcgaggc gggcgaatcg tctccgtttc
     1261 acagccgcgc tacgagcgta tcgtccggtt cagtatagcc aagcccttcc ggcccaacaa
     1321 ctcggacgac gatgaggacg acgatggaga cgacaacatc gaactcgtga caaccgacct
     1381 gtacgtcgaa ctgatggggc ggcgctcgaa tatcatcctc accgacgaca gtcgacggat
     1441 cctcgacgcc gtcaagcgcg tctcgccgga gatgagccgc gtcaggccga tccgtcccgg
     1501 cgctgcctac gttccacccc cgccgcagga caagcaagat cccctgcgcg ccacgccaca
     1561 cgggctgctt gatgcggccg gcgagtcgcg cgagccgctg gcgaaatggc tcgtcgcaac
     1621 ctttcgcggg atgagcccgg cgctcgctgc cgagaccgcc gttcgagccg ggatcgagcc
     1681 agacactcgc gccgcccagt tgagcgggga cgacgcggcg cgtcttgtca gcgcgctgac
     1741 ggcgatgttc ggcggtatcc agaccggtga gtggcagccg atgctctata gctgggacac
     1801 cggcagggca gatttcttcg ccatcgagat gcagtccctc gcgaacgagg caggcgtcac
     1861 ggccaccgtc tacccatcga tcgttgaggc ggccgaagcg gcctgggagg ccaacctgac
     1921 agccgtgtcg gcgtcagcgg gacggcacac agttcgccgc gaacggctgg tggccgagat
     1981 agacgaagcg cgcgcgcgcg tccgccagcg aacccactcg ctggaggagc aggtcgcccg
     2041 cgcggccgag gtcgaggtgc tacgcaccaa aggcgagatg atttacgcct ggatcggcga
     2101 gattcggcag ggccaatcga actacacaac accggatggc ctgacgatcg agctcgaccc
     2161 gacgctttcg cccagccaga acgcgcagga ttacttcgag cgctaccgca aggcccaatc
     2221 ggcggaggcg aacctgccgg cgctgctcga cgaagcggcc cgcgagctgg cctacctcga
     2281 ccagcttcgc tcgatggcgg cgctggccga gacgtacgac gagatcgagt cggtccgcct
     2341 cgaatggctg gcctgggcgg aaacggcccg tggaggcgcc cgtgcatcgc ggccgaaagg
     2401 tgcgcgcccc tcgaagcagg cccgccgccc gcgcgcctac cgcaccagcc gcggcgacgc
     2461 catctacatc ggacgaaccg gccagcagaa tgacgaggtc accttctcga tcgccaatcc
     2521 cgacgacctc tggctacatg ttcgcaacat gcccggcgcc cacgtcatcc tgcgcgccaa
     2581 cggccaggtc tccgacgaga cgattgagcg ggccgcctca ctggccgcct ggtacagcga
     2641 cggccgcaac gcaaccgccg tcccggtcga tgtcaccgaa cggcgccacg tccgcaagat
     2701 caaaggcgcc ggacccggga tggtcaccta ccgcaatgaa cgcacgctca acgttcgccc
     2761 gctgtcggag aaggaactgg ggctggaagg aaccgggtaa tctgccctgt cgtcctaagc
     2821 cgcagcgaag aacctctccg tgctagccac ctatggcacc gaagcggaga ggtccttcgc
     2881 tgcggctcag gacgacaggg ggttactcct gcgagcgcaa tgatttgcgg acggttcgcg
     2941 agagcaggat gccgatttcg taaagcgcgt acgtcgggat agcaaccagc atcatgttga
     3001 agggatccgg cgtcggcgtg atgattgcgg ccgcgatcat gccgagaatc agcgcgtact
     3061 tgcggaagcg cccgtagcgc ttcgcggaca tgatgccaat tagcgccagg gcatacatga
     3121 cgaccggaag ctcgaagatc aggcccatgc cgagcatcaa cgtcatgtag aactgcacga
     3181 tctcggacgc gcgtgggtcc cagcggaaat agtcgatgcc gaacgtcgac agccactgga
     3241 gcatgcgggg agcgaggacg aagaatgcga acgcggcgcc ggcgcagaac atgatgaaga
     3301 cgaacggaac cgcgcggatc acgtagcgcc gttcgcgcct cgtcaggccc ggcaccagaa
     3361 atgccatcaa ctggtagatc agcaccggca tcgccagcgc gatggcgatg tagaagacga
     3421 ctttgaacca ggtgacgaaa ccctcggtcg ggccg
//
LOCUS       DASUGJ010000596         4407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1765007, whole genome shotgun sequence.
ACCESSION   DASUGJ010000596 DASUGJ010000000
VERSION     DASUGJ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765007"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFV93_13520"
     CDS             <1..205
                     /locus_tag="VFV93_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166415.1"
                     /translation="AKAALHGTLPQRPTSHPRQLPGTAIVDPSGRLRYLHHARDAADH
                     LTSRQLIDLAREHAAQPETPVTA"
     gene            274..1542
                     /locus_tag="VFV93_13525"
     CDS             274..1542
                     /locus_tag="VFV93_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166416.1"
                     /translation="MNRPNWTISGRTLFSLTLIGSLLLTACSGTDNDDPTATAPPASP
                     TATIEPSPTATATLEPTATATLEPTATATATATATLEPTATPTTEPTATATVAATAVP
                     LPTGGAELRILGKAIQGIVPGNSDGSVLYATTAVGLSRSRDGGRTWTASGDNQQGVMV
                     AALNNPEVLYAGDYGVCSQGSAGGMMTRSVDGGFTWEEFANGAGIRPLLVEAGQQSMV
                     IGSDCALQISSNGGQNFSTFTDHGNADIYAAASSDPAALDDQMVILGIAEGGTGHLFL
                     YDLSSQKPSFEGDLAEFFGLGAVAWSGGRLVLATSTGVGVSDDNGATWSWSRAGLEDA
                     TYSVNPLEEPVPDDEAGKSLGFTVVAIDPDDPDRIWIGGQLGAFSSSDGGQTWQQLGD
                     NSPIDTLVVATATNSVFVSSDGGTRVWAIE"
     gene            1749..2576
                     /locus_tag="VFV93_13530"
     CDS             1749..2576
                     /locus_tag="VFV93_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5166417.1"
                     /translation="MSSAIDFTGKVAIVSGAASGICRVILTRLAEAGAKAVIADLDDE
                     WGTSTAHDLAGRGLDVIFSATDVRDSSQVNRTIEAAVERFGRVDILVHGAGVGVHKEI
                     VDLSDEEWDLQIDVQLRGAFLLSRAAARQMIRQGEGGRIILIGSTSANNARPKSGPHA
                     ASKAAEIQLSRVMALELGRYGITSNVVSPGLTNIEGISRSSQSPEYQKVFVTQVPLGR
                     LGTPDEIADAVLFIASDQARFITGQAIYVDGGYSAGKLVVDGPHRPEAHGQLPAESS"
     gene            2618..3715
                     /locus_tag="VFV93_13535"
     CDS             2618..3715
                     /locus_tag="VFV93_13535"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX5166418.1"
                     /translation="MKIHIVGSGAIGGMAGAWMVKHGEDVTFVDAWREHVDAIKADGL
                     RIDGILGPHQFDVRALHPEELDEPIEMVFVATKSHHTDSAIDLIRPHLTPDATVVSLQ
                     NGFNAERIANAIGAQHVIGTVPDYTAALVAPGFLEYTVVGPAYIGELDGSSTPRLAEV
                     HRLLSMLTTTNVTTNIVGRIWTKQCYMSWIVMSAMVDAPIVEVLRPIRNRLLGVAVVR
                     ETLPIAEQSGVSLESDKYFQQELLARRDADARQQQSDVIQGLIDHFDHRSNEPQAREE
                     YRYVKQGSGMWWDIVYRKRPSETRWITGALVDRAQQLGLPAPLNTAMAEMVYAIERGE
                     RPLGWDNLDELASLAASLAEPLGLEGQGGER"
     gene            3712..>4407
                     /locus_tag="VFV93_13540"
     CDS             3712..>4407
                     /locus_tag="VFV93_13540"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5166419.1"
                     /translation="MTESQSRTTLLRGGTLIDGSGAAPSPDSAVLVVDERIEWTGPAA
                     AAVAPPGTVEIDVRGKTIMPGLIDCHDHMVHTGFDLMRRASAPLSLTMMRIADNLRVT
                     LEAGITTVRDAGGLDVGFKMAVEEGLIPGPRLVLGLSILSRTGGIADPRMRSGNVLEF
                     MELPGLPSPVRDGVEECRKGVREVIHAGADWVKCASTGGVSSPTLGPVDAALTFDELQ
                     VIADEAHMRDKRVF"
BASE COUNT          782 a         1488 c         1424 g          713 t
ORIGIN      
        1 ggcgaaggca gccctgcatg gcacgctgcc gcaacggccg accagccacc cgcgccagtt
       61 gcctgggacg gccatcgtcg atccatctgg ccggctgcgc tacctgcacc acgcccgcga
      121 cgccgccgac cacctcacca gccgccagct catcgacctt gcccgggagc atgcggccca
      181 gccggaaaca cccgtcaccg cgtaacgcgg ctcttgctga cgtgtttcag tccgtggcgg
      241 catcattgtg tacctggcat gcgaagggtg agcatgaacc gaccaaactg gaccatttct
      301 ggtcgaactc tattcagcct gacactgatc ggatctctct tattgacggc ctgttccggc
      361 actgacaacg atgacccgac cgcaacggcg ccgccggcca gcccgacggc gacgatcgag
      421 ccatcgccga cggcaacggc aacactggag ccaactgcga ccgccacgct cgaaccgacc
      481 gccaccgcga cggccacagc gaccgccacg ctggaaccga ccgcgacgcc gacgaccgaa
      541 ccgaccgcga cggcgaccgt cgcggcgacg gctgtgccgc taccgaccgg cggcgcggaa
      601 ctgcgcatcc tcgggaaggc gattcagggg atcgtgcctg gcaacagtga cggcagcgtc
      661 ctctatgcga cgacggcggt tggcctctcc cgttcccgcg acggcgggcg aacctggacg
      721 gcctccggtg acaaccagca gggcgtgatg gtggccgccc tcaacaaccc cgaggtgctc
      781 tacgccggcg actacggcgt ctgctcgcag ggttcggccg gcgggatgat gacgcgctcg
      841 gtcgatggcg gctttacctg ggaggagttc gccaacggag ccggcatccg gccgctgctg
      901 gtcgaggccg gccagcaatc gatggtgatc ggctccgact gcgcgctcca gatctcgtcc
      961 aacggcggcc agaacttcag caccttcacc gaccacggta acgccgacat ctatgccgcc
     1021 gccagctccg acccggccgc gctcgacgac cagatggtta tcctcggcat cgccgaaggc
     1081 ggcaccggcc acctcttcct ctacgacctc agcagccaga agccatcctt cgagggtgat
     1141 ctggccgagt tcttcggcct cggcgccgtc gcctggtcgg gcgggcgact cgtcctggcg
     1201 acatcgaccg gcgtcggcgt ctccgacgac aacggcgcga cctggagctg gagccgcgcc
     1261 ggtctggagg acgccaccta ctcggtcaac ccgctcgaag agccggtccc cgacgacgaa
     1321 gccggcaagt ctctcggctt caccgtcgtc gcgatcgatc ccgacgaccc cgaccggatc
     1381 tggatcggcg gccagctcgg cgccttcagc agcagcgacg gcggccagac ctggcagcag
     1441 ctcggcgaca actccccgat cgacacgctg gtcgtcgcta cggccaccaa cagcgtcttc
     1501 gtctcctccg acggcggcac ccgcgtctgg gcgatcgagt gagcgcccag acgccagatt
     1561 gcccgccacc accatcgtca tgatggtggc gtaaaggccg agaaaccggg atactgtcgg
     1621 gatccgtctg ttggtccgag tacaaatcga gtattgtgtc gtgagaacgc tcaacgaaca
     1681 catcaccgcg gagagttgga tgctgccgct ggcggaccca gtttgtctca ggatgacggg
     1741 agatgacgat gtcctccgcg atcgatttca ccggcaaggt cgcgattgtg tccggcgctg
     1801 cgtccgggat ctgccgcgtc atcctgaccc ggctcgccga ggccggcgca aaggccgtca
     1861 tcgctgacct cgacgacgag tggggaacgt cgaccgcgca cgatctggcc ggccgtgggc
     1921 tggacgtcat cttctcagcg actgacgttc gcgacagcag ccaggtgaac cgaaccattg
     1981 aagcggccgt cgagcgcttt ggccgtgtcg acattctggt ccatggcgcc ggtgttggcg
     2041 tccacaaaga gatcgtcgat ctctcggatg aagagtggga tcttcagatc gatgtccagt
     2101 tgcgcggcgc ctttctcctc agccgtgctg ccgccaggca gatgatcagg cagggcgagg
     2161 gcgggcgcat cattctcatc ggctcgacat cggccaacaa cgcgcgcccg aaatccgggc
     2221 cacatgccgc gtcaaaggca gccgagatcc agctctcacg ggtcatggcg ctcgaacttg
     2281 gccgctacgg catcacctcc aatgtcgtct cgccgggcct gaccaatatc gaaggcatct
     2341 cgcgttcctc gcagtcgcct gagtatcaga aggtgttcgt tacacaggtt ccgcttggcc
     2401 ggctaggcac accggatgag atcgccgacg ccgtgctgtt catcgcgtcc gaccaggcgc
     2461 gcttcatcac cgggcaagcg atctacgtcg acggcggcta ctccgccggc aaactagtgg
     2521 tcgacggccc gcaccggccc gaggcgcacg gccaactacc tgccgaatcg tcgtgattcg
     2581 ctgacgtcgt caccgacggt ctcgtgaaag agaacagatg aagattcaca tcgttggatc
     2641 aggcgcgatc ggcggcatgg ccggcgcctg gatggtcaag cacggcgagg atgtgacgtt
     2701 cgtcgatgcc tggcgcgagc atgtcgacgc gatcaaggct gacggactgc ggatcgacgg
     2761 tatcctcggc ccgcaccagt tcgacgtgcg cgcactgcac cccgaggagc tggacgagcc
     2821 gatcgagatg gtgttcgtcg ccaccaagtc gcaccacacg gacagcgcga tcgacctgat
     2881 caggccgcac ctcaccccgg atgccaccgt cgtctcgctc cagaatggtt tcaacgctga
     2941 acgtatcgcc aacgcgatcg gcgcgcagca tgtgatcgga accgtcccgg actacacggc
     3001 cgcgctggtc gcgcccgggt tcctggaata caccgtcgtc gggccggcct acatcggcga
     3061 gctggatgga tcatcgacgc cgcgcctggc ggaggtccat cggctgctgt cgatgctgac
     3121 gacgaccaac gtcaccacca acatcgtcgg ccggatctgg accaagcagt gctacatgtc
     3181 atggatcgtc atgtcggcga tggtcgacgc gccgatcgtc gaggtgctca ggccgatccg
     3241 caaccggttg ctgggtgtcg ccgtcgttcg tgagacgctc ccgatcgccg agcagtcggg
     3301 tgtcagcctg gagagcgaca agtatttcca gcaggagttg ctggcacggc gtgacgcgga
     3361 cgcccggcag cagcaatccg atgtgatcca gggcctcatc gaccacttcg accatcgctc
     3421 caacgagccg caagcccgcg aggagtatcg ctacgtcaag caaggcagcg ggatgtggtg
     3481 ggacatcgtc tatcgcaagc gcccgagtga gacacgctgg atcaccggcg cgctggtcga
     3541 tcgggcccag cagcttggcc tgccggcccc gctgaacacc gcgatggccg agatggtcta
     3601 cgcgatcgag cgcggcgagc ggccactcgg atgggacaac ctcgatgaac tggcctcgct
     3661 ggccgcgtca ctcgctgagc cgctcgggct ggaaggccag gggggtgagc gatgacggag
     3721 agccagtcac gaacgacttt gctgcgtggc gggacgttga tcgacgggtc gggcgccgcg
     3781 ccgagtccgg acagcgccgt cctggtcgtg gacgagcgga tcgagtggac cggcccggct
     3841 gcagccgcag ttgcgccgcc ggggacggtc gagatcgatg tgcgtggcaa gacgatcatg
     3901 ccggggctga tcgactgcca cgaccacatg gtccacaccg gcttcgacct gatgcgacgc
     3961 gccagcgcgc cgttgagcct gacgatgatg cgcattgccg acaacctgag ggtcacgctc
     4021 gaggccggga ttaccaccgt ccgcgatgcc ggcggcctgg atgtcggctt caagatggcg
     4081 gtcgaggagg gactcattcc cggcccgcgg ctcgtgctcg gtctctcgat tctctcccgt
     4141 accggcggaa tcgccgaccc gcggatgcgt tccggcaacg ttcttgagtt tatggagctg
     4201 ccgggactgc cgtcgccggt ccgcgacggt gtcgaagagt gccgcaaggg tgtccgcgag
     4261 gtcatccacg ccggcgcgga ctgggtcaaa tgcgccagta ccggcggcgt cagctcccca
     4321 acgctcggac cggttgatgc agcgctgacc ttcgacgaac tgcaggtgat tgcggacgaa
     4381 gcccacatgc gtgacaagcg ggtgttc
//
LOCUS       DASUGJ010000597         5080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_652759, whole genome shotgun sequence.
ACCESSION   DASUGJ010000597 DASUGJ010000000
VERSION     DASUGJ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652759"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..787)
                     /locus_tag="VFV93_13545"
     CDS             complement(119..787)
                     /locus_tag="VFV93_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166420.1"
                     /translation="MLRVVIADDHDLFREGLKQLLESVEDITVVGEASDGKQAVFLVE
                     KHRPDVVLMDISMPEMDGIQATESIQARGLGTPVIVLTMYADDEYAIHAIRAGAKGYL
                     LKNTRSDEVIRAIRLAAAGGSAIDPALGAVLMREFQRLLNRTPGEARQQLSSREQQFL
                     ELLVKGHNNRQIAQELDLAESTVKNNLSALFQKIGVRDRTQAVLYAISNGIVAPPTDV
                     ASRS"
     gene            complement(828..2141)
                     /locus_tag="VFV93_13550"
     CDS             complement(828..2141)
                     /locus_tag="VFV93_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5166421.1"
                     /translation="MQVTNQHPVIQTPRGRGETAALDRMLRNSLASFPFDLQTRLVDA
                     RSVQDVYEIGVAMLARIPGTVGVRIFQRNDSGELIVVHAWHSPLAKPSGVSNEELAQV
                     THKRWIETHGVGRSLHEFARPFADPQYQSWLMVPMLFGDELIGAIAAERRDGQTFAYA
                     PEDIVAFAAAAAGMAWGIQAIFLRDRTDIVSQQEAREDLLAQERREVGRELHDNVVQD
                     LAYVNLKLELADKYTSNDPSMVQGEIQAARELLDHAITELRRTIGELRRPAPARRGIT
                     GQLRALVTHIEPEVPEFEVDFKQISGVQLVPELERAVVGIVREAIQNVRKHANAQSVR
                     LEVRRADDELRLYVIDDGVGMQDKGPNGHFGMEIMRELAEDLGGTLNIDSKSGEGTKI
                     EAYLPLVLPAVSAFNDSTGLAAAELSTRPTTTTIGARDADAMAGS"
     gene            complement(2128..4506)
                     /locus_tag="VFV93_13555"
     CDS             complement(2128..4506)
                     /locus_tag="VFV93_13555"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5166422.1"
                     /translation="MTTDHSKITFLPTADVNRIFSRDEEAGVTPLPFPTAIARVAHAV
                     SSDTSFDDLLHEIVVSASRAIDADIGMIRAISSDGANFVVMAANGPAPLSIGGLIGSY
                     SVISEDVSAATPGSTITIDLRSEAQVARLPSNERWDFEHIGGRFLLIVPLFARGRLVG
                     RLDLVRTANGPFSMESRNLVLPFAAYAAGTLLDQQHHRASEESQVFQTVIRLHESVEQ
                     LADPETILQAVVELIVREPGCARCYAMLWNIERGEFVPTAVAGLEPHLVDILKLISLS
                     PQVVPAFDQMVHSSRPLVIADATKSTLLPASLVRALGIRAAMIVPLRGRRHQTTGVLL
                     LDQNEEGIQFTDSQVTVMAGMAQHLSTLIENAILFDEIRGNSDSMSIINEIAIQMAML
                     TDEDSLFRQLYYQVASVLDAGRFGMALLTPDRRALDMRAAIDGIVSEGTTRMPLGDDP
                     LSSVSASGRVELVGIRSGADNREWLPGVVPEDPPHSHLTVPITVGRNVIGALSIQSPF
                     RNAYSPRDRELLLAVALHTGIAIENARLYRIVQSRGDRRAAVLDEVMQRQELERKELV
                     DDIHDHTLQTLAACLYRLDQAQSTVSMLSEHEGALVQINDVRESLSENIDRLRRRIFE
                     LRPATLDRLGLEPALRELLSGIGQDLDLMADLDTDLPTRLAPEQETLVYRLVQEALDY
                     IIARDGVSSIDVSMREHGDNILIAIHDDADYRADTTTESAEGVPDIGLLALIERADLA
                     GGHLRVSRRSAGGSMMEITLPGAPRDEDVAPDPNTNLSLVEAAMEGGRDAGD"
     gene            4791..>5080
                     /locus_tag="VFV93_13560"
     CDS             4791..>5080
                     /locus_tag="VFV93_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5166423.1"
                     /translation="MQITFLGTGTSNGIPVIGCTCPVCTSSDPRDKRSRTSAVVEVDG
                     RTLLIDTSPELRLQAIANGLTHVDAVLFTHAHADHIAGFDDLRSFNFLSQAPL"
BASE COUNT          865 a         1590 c         1687 g          938 t
ORIGIN      
        1 gacggtaaag cactcaagga ctatccagca gcaaaggcat tcttcgaagc gcagtcgtaa
       61 gccaaataga caacgacccc acccacgagt gggtggggtc gttgcttggc gccgcaggct
      121 agctgcgcga ggcgacgtcg gttggtgggg cgacgatgcc gttggagatc gcgtagagga
      181 cggcctgggt gcggtcgcgg acgccgatct tctggaagag cgcgctcagg ttgttcttga
      241 cggtgctctc ggccaggtcc agctcctgcg cgatctggcg gttgttgtgg cccttgacga
      301 ggagctccag gaactgctgc tcgcgcgagg agagctgctg gcgcgcttcg ccgggtgtgc
      361 ggttgagcaa ccgttggaac tcgcgcatca ggaccgcgcc gagggccggg tcgatcgccg
      421 agccaccggc ggcggccagc cggatagcgc gaatgacttc gtccgagcgg gtgttcttca
      481 gcagatagcc tttggcgccg gcgcggatgg cgtggatggc gtattcgtcg tcggcgtaca
      541 tcgtgaggac gatgacgggc gtgccgaggc cacgggcctg gatcgactcg gtcgcctgga
      601 tgccgtccat ctccggcatg ctgatgtcca tcagcacgac atccggccgg tgcttctcga
      661 ccaggaagac ggcctgcttg ccgtcgctgg cttcaccgac gacggtgatg tcctcgaccg
      721 attcgagaag ctgtttcagc ccctcgcgga acagatcgtg atcgtcagca atgactacac
      781 gtagcatgac tggtctgtgt cctcaccgtt gccctggtgg aggggagtca actccccgcc
      841 atcgcatccg catctcgcgc gccaatagtt gttgtagtcg gccgggtgct gagctccgcc
      901 gctgccaggc cggtgctgtc attgaatgcc gagacggccg gtagcaccag cggcaggtag
      961 gcttcgatct tcgtgccttc gcccgatttg gagtcgatgt tcagcgtccc gccgagatcc
     1021 tcggccagtt cgcgcatgat ctccatcccg aaatgtccgt ttggcccctt gtcctgcatc
     1081 ccgacaccgt cgtcgatcac gtacaggcgg agttcatcgt cggcccgtcg tacttcgagc
     1141 cggaccgact gcgcgttggc gtgtttgcgc acgttctgga tcgcctcgcg gacgatcccg
     1201 accaccgccc gttcgagttc gggtacgagc tggacgccgg atatctgctt gaagtcaacc
     1261 tcgaactcgg gaacctccgg ctcgatgtgg gtgaccagcg cgcgtagctg gccggtgatg
     1321 ccgcgccggg ccggtgcggg ccggcgcaac tcgccgatcg tccgccgcag ttcggtgatg
     1381 gcgtggtcga gcagttcgcg cgctgcctga atctcgccct ggaccatcga cgggtcgttg
     1441 ctggtgtact tgtcggccag ttcgagcttg aggttgacgt aggcgagatc ctggacgacg
     1501 ttgtcgtgta actcgcggcc cacctcgcgc cgttcctggg cgagcaaatc ttcgcgcgct
     1561 tcctgttggc tgacgatgtc ggtccggtcg cgcaggaaga tcgcctgaat gccccacgcc
     1621 atcccggcgg cggctgctgc gaaggcgacg atgtcctccg gtgcgtaggc gaaggtctgg
     1681 ccgtcgcgcc gttcggcggc gatcgcaccg atcagctcat cgccgaacag catcggaacc
     1741 atcagccacg attggtactg cgggtcggca aatggccggg cgaactcgtg caggctgcgc
     1801 ccgacgccgt gcgtctcgat ccagcgcttg tgggtgacct gcgccagttc ctcgttggag
     1861 acgcccgacg gcttggccag cggtgagtgc caggcgtgga cgacgatcaa ctcgccgctg
     1921 tcgttgcgct gaaagatgcg aacgccaacc gtcccgggaa tccgcgccag catcgcgacg
     1981 ccaatctcgt agacatcctg gacggagcgt gcgtccacca gccgcgtctg gaggtcgaat
     2041 ggaaagctcg ccagcgagtt gcgcagcatc cgatcgagcg cggcggtctc accgcgccct
     2101 cgcggggtct gaatgacagg atgctggtta gtcacctgca tcacggcccc cttccatggc
     2161 tgcctcaacg agcgagaggt tggtgttcgg atcgggtgcg acatcctcgt cgcgtggcgc
     2221 gcccggtagc gtgatctcca tcatcgagcc gcccgcggaa cggcgagaga cacgcaagtg
     2281 gccaccggcc aggtcggcgc gctcgatgag tgccagcagg ccgatatcgg gaacgccttc
     2341 ggctgattcg gtcgtcgtgt cggcacggta gtcggcgtcg tcatgaatcg cgatcaggat
     2401 gttgtcgccg tgctcgcgca tcgagacgtc gatcgaggac acgccatcgc gggcgatgat
     2461 gtaatcgagc gcctcctgga cgagccggta gaccagcgtc tcctgctccg gcgccagccg
     2521 cgtcggcaga tcggtgtcga ggtcggccat caggtcgaga tcctgaccga tgccgctcaa
     2581 cagctcacgc aacgccggtt cgaggccgag ccggtcaagc gttgccggcc gaagctcgaa
     2641 gatccgtcgt cgcaggcgat cgatgttctc cgacaggctc tcacggacat cgttgatctg
     2701 gaccagcgcg ccctcatgct cgctgagcat cgaaacggtc gattgcgcct gatcgagccg
     2761 gtagagacag gcggccagcg tctgcagggt gtggtcgtga atgtcgtcga ccagctcctt
     2821 gcgctccagc tcctgccgtt gcatgacctc atcgaggacg gctgcccgtc ggtcgccccg
     2881 tgactggacg atccgataga gccgggcgtt ctcgatcgcg atgccggtgt gcagtgcaac
     2941 ggccagcagc agttcgcggt cacgcgggct gtaggcgttg cggaagggcg attgaatcga
     3001 aagcgcgcca atgacgttgc ggccgacagt gatcgggacc gtcagatgcg agtgcggtgg
     3061 gtcctccgga acaacacccg gcagccattc gcggttgtcg gcgccgctgc ggatgccgac
     3121 caactcaacg cgcccgctgg cggagacgct ggagagcggg tcgtcgccga gtggcattct
     3181 ggtcgtaccc tcgctgacga tgccgtcgat ggccgcccgc atgtcgagcg cccgccggtc
     3241 aggcgtcagt aacgccattc cgaagcggcc ggcgtcgagg accgaggcga cctggtagta
     3301 gagctggcgg aagagactgt cctcgtcggt cagcatcgcc atctggatcg cgatctcgtt
     3361 gatgatcgac atgctgtctg agttgccgcg aatttcatcg aacaggatcg cgttctcgat
     3421 caacgtcgag aggtgctgcg ccatcccggc catcactgtt acctgcgaat cggtgaactg
     3481 gatgccctct tcgttctggt cgagcagcag gacgccggtc gtctgatggc ggcggccccg
     3541 caacggcacg atcatcgcgg cccggatgcc gagcgcgcga accagcgacg ccggcagcag
     3601 ggtggacttg gtcgcatcgg cgatcaccag cggacggctg ctgtggacca tctgatcgaa
     3661 ggcaggaacg acctgcggcg agagcgagat cagcttcagg atgtcgacca ggtgcggctc
     3721 aagaccggcg acggctgtcg gcacgaactc gccccgctcg atgttccaga gcatcgcgta
     3781 gcagcgcgca cagcccggct cacggacgat caactcgacc accgcctgga gaatcgtctc
     3841 cggatcggcc aattgctcaa ccgactcatg caggcggatg acggtctgga agacctggct
     3901 ctcctccgac gcgcggtgat gctgctgatc cagcaacgta ccggcagcgt aggcggcgaa
     3961 gggcaggacg aggttgcggg actccatcga gaacgggccg ttggcggtcc ggacgaggtc
     4021 gagccgcccg accaaccggc cgcgcgcgaa gagcggcacg atcagcagga agcggccgcc
     4081 gatatgctcg aagtcccagc gctcgttaga tggcagccgc gcgacctgtg cttcggagcg
     4141 caggtcgatc gtgatcgtgc tgccgggagt ggccgcgctg acatcttcag agatgactga
     4201 gtaggagccg atcagcccac cgattgagag tggcgccggg ccgttggcgg ccatgacgac
     4261 gaagttcgcg ccgtcggacg agatcgcgcg gatcatgcca atgtcggcgt cgatggcgcg
     4321 gctggctgag acgacgatct catgcagcag atcgtcgaac gacgtgtcgg agctgacggc
     4381 gtgggcgacg cgggcgatcg cggtcgggaa cggcaacggc gttacccctg cctcctcgtc
     4441 ccggctgaaa atccggttga cgtcggcggt tggtaggaag gtaattttcg agtgatctgt
     4501 tgtcatgccg ttcgttccca ctgcgtccgt ttgggcggtt cataccgaac ctgaccacgg
     4561 agtagtccga ggtggccggc cggagcgacc tggcagccct gttggctact atcatcatat
     4621 cgagccagat tggcgcgaca atgccgtcgg agtaccctat tggtaccgtc tttccctcac
     4681 acgctaacca tgaccccaac cgcgagtggt tccggccaaa cgtatacttc attgccgctt
     4741 gacggacgcg ttgcaggaac acgcgagggc ttaatgactg tcaggagcat atgcagatca
     4801 cgtttctcgg caccggaacc agcaacggta tccccgttat cggctgcacc tgtccggtct
     4861 gtacatcatc cgacccgcgt gacaagcggt cgcgaacctc ggcagttgtc gaggttgacg
     4921 gtcggacact cctgatcgac acctcgccgg agttgcgcct gcaggcaatc gccaacggac
     4981 tcacgcatgt cgatgcggtc ctcttcactc acgcgcatgc cgaccatatc gccgggttcg
     5041 acgatctgcg cagcttcaac ttcctcagcc aggcgccgct
//
LOCUS       DASUGJ010000598         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_653105, whole genome shotgun sequence.
ACCESSION   DASUGJ010000598 DASUGJ010000000
VERSION     DASUGJ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653105"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VFV93_13565"
     CDS             <1..845
                     /locus_tag="VFV93_13565"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5166424.1"
                     /translation="AAQDDGAYHHELTTAIAVAREAGALLRERFGHVRDVQYKGLVDP
                     VTDADKASEALIAERLLAEYPDDRLLAEEGSGAGENTGAERLWIVDPLDGTVNFMHGL
                     PVFAVSIALRAGDETVVGVIYDPLRDELFAAQRGGGATLNDQPMQVSETSELLRALLV
                     TGFAYDVQWRTENLAHFARFTDATQAVRRDGSAALDLAYVAAGRYDGYWERGIVAWDI
                     AAAALMLTESGGIVSGYEGQPFDPFAREIVASNGKIQAAMLEIIAGVEAEAPDRLPRG
                     VNFS"
     gene            960..1400
                     /locus_tag="VFV93_13570"
     CDS             960..1400
                     /locus_tag="VFV93_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166425.1"
                     /translation="MDNIFDTYHERIIEAIREGDVVCIFFPRLGKTLILDLRQSIETP
                     AAIMIDNMVSNARERLESLQRMRPEFPMPDEVRLAPWVGFVRSMQETGVLDAMIDRCR
                     QTGDSSLVQECRDAFEALERNERQLVRAIVRGDTTRTIWQRPRA"
     gene            1460..2236
                     /locus_tag="VFV93_13575"
     CDS             1460..2236
                     /locus_tag="VFV93_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5166426.1"
                     /translation="MTKPVTTNLPEPPDADLQPFEFTESPAGLAEVVPAFDQSLAGTE
                     EEFIAQTLGWCAELFGASRARFIRPLESGNWVVYTRQQERETVLTHLADQAEIAMAYT
                     VGLSKQPLVVTRPRISHFDGSGLRPIALKSYLGIPIVCQDRLVGVVEFAGEVRPDMES
                     ALYSAAPRLANVGERLLFDPGLRRHRETLTAGSSVVLSGGVRSRGDILISAEELSFLA
                     AINGPTTLATLASATGMAIDLAIKLAADLRERGLITICSE"
BASE COUNT          449 a          840 c          842 g          393 t
ORIGIN      
        1 ctgcggctca ggacgacggg gcgtaccacc acgaactcac caccgcgatc gccgttgctc
       61 gcgaggcggg cgcgttgctg cgagagaggt tcgggcatgt gcgcgatgtc cagtacaagg
      121 ggctggtcga tcccgtcacc gatgcggaca aggccagcga ggcgttgatc gcggagcggc
      181 tgctggcaga gtatccggac gaccggctgc tggccgagga gggcagcggg gccggcgaga
      241 ataccggcgc tgagcggctc tggatcgtcg acccgctgga tggcaccgtc aacttcatgc
      301 acggcttgcc ggtcttcgcc gtcagcatcg ccctgcgggc cggcgacgag acggtggttg
      361 gcgtcatcta cgatcccctg cgcgatgagc tgttcgcggc ccaacgcggt ggcggcgcga
      421 cgctcaacga ccagccgatg caggtctcgg agacgagcga gctgctgcgg gcgctgctgg
      481 tgaccggctt cgcctatgat gtgcagtggc ggaccgagaa tctggcccac ttcgcgcgct
      541 tcaccgatgc gacgcaggcc gtccggcggg acggctcggc cgcgctcgac ctggcctacg
      601 tcgcagccgg ccgctacgac ggctactggg aacggggcat cgtcgcctgg gatattgcgg
      661 cggcggcctt gatgctgacc gaatccggcg gtatcgtgag cggatacgaa ggccagccgt
      721 tcgacccgtt cgcgcgcgag atcgtcgcca gcaacgggaa gatccaggcg gcgatgctgg
      781 agatcatcgc cggtgtcgag gccgaagcac cggatcggtt gccgcgcgga gtaaacttca
      841 gttgatcccg aggcaacgcc tcccggccaa ttccttgcgt cgggatggca gggacgatcg
      901 aacgacaacc ggctgatacg acgacgacac caattcgccg atcccaggag cgcgtggcaa
      961 tggacaacat cttcgatacc tatcacgaac gcatcatcga agcgatccgc gagggcgatg
     1021 tcgtctgcat cttcttcccg cgcctcggca agacgctgat cctcgacctg cgccagtcga
     1081 tcgagacgcc ggccgcgatc atgatcgaca acatggtctc gaacgcccgc gagcggctgg
     1141 agagcctgca acggatgcgg cccgagttcc cgatgcccga cgaggtccgg ctcgcgccgt
     1201 gggtcggctt cgttcgctcg atgcaggaga ccggcgtcct cgacgcgatg atcgatcgct
     1261 gccggcagac gggcgattcg agcctcgtcc aggaatgccg cgacgcgttc gaggcgctcg
     1321 aacgcaacga acggcaactc gtccgtgcga ttgtccgggg cgacacgacc cgcacgatct
     1381 ggcaacgccc acgcgcctga gacactgatc cacttgcagc ccaggctgat cccgatcctg
     1441 agacggagcg acgaccggca tgaccaagcc cgtgacaacc aacctgccgg aaccgcccga
     1501 cgcggatctg cagccattcg agttcacgga gagcccggcc gggctggctg aggtggtgcc
     1561 ggccttcgat cagagcctgg ccgggaccga ggaagagttc atcgcccaga cactcggctg
     1621 gtgcgcggag ctgttcggcg cgagccgggc gcgcttcatt cgcccgctgg aatccggcaa
     1681 ctgggtcgtc tacacccgcc agcaggagcg cgagacggtc ctgacccatc tggccgatca
     1741 ggccgagatc gcgatggcct acacggtcgg cctcagcaag cagccgctgg tcgtcacccg
     1801 cccccgtatc tcccacttcg acggcagcgg gctgcggccg atcgcgctca agagctacct
     1861 cggcatcccg atcgtctgtc aggaccggct ggtcggcgtc gtcgagttcg ccggcgaggt
     1921 ccggccggac atggaatccg cgctctactc ggccgcgccc cggctggcga acgtcggcga
     1981 gcggctgctc ttcgatcccg gcctgcgccg ccaccgcgag acgctgacgg ccggctccag
     2041 cgtcgtcctg tccggcgggg tccgctcgcg gggcgacatc ctcatcagcg ccgaggagct
     2101 gagcttcctg gcagccatca acggccccac cacgctcgcc acgctcgcca gcgcgaccgg
     2161 catggccatc gacctcgcca tcaaactggc cgccgacctg cgcgagcgcg gcctgatcac
     2221 gatctgctcc gagtaacaac agccagcgaa cgagatgggc gcagatccct agccgcagcg
     2281 aaggttctcg tctgcgttcg gtttgtgcac gcgtcaacgg cacagagacc cctccggcga
     2341 tgggatgaga gaggaagcag ggcgatcgcg atgacatgac aatatttgtg ataacagcga
     2401 ctctgtgtca cagcctacca attcacagcc acatcctgta tgatgtcgat gatggaaaag
     2461 atgtaccggt acagcgtccc atccgtacgc cggtggagag gcgggctgtg gaaaaggtgg
     2521 ggac
//
LOCUS       DASUGJ010000599        11418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1070552, whole genome shotgun sequence.
ACCESSION   DASUGJ010000599 DASUGJ010000000
VERSION     DASUGJ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11418
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070552"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFV93_13580"
     CDS             complement(<1..580)
                     /locus_tag="VFV93_13580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5166427.1"
                     /translation="MMTASAEPGDGRSADDAIDHAALNELSSDLIQVASVNGLLRYVN
                     SAWRRALGYGDQDISRILLLDLIHPNHQNSFAETMRSVIQQRAAHNVSTVLLTRTGEP
                     LSVSGAMDCQVAAGRPVLLRAVMRDARSDANLDLLNGQWTSVFESPAIGMSVQSLDGR
                     WLAVNDAMAGILGYSPSELIAMTFHDITHPDDI"
     gene            complement(729..1985)
                     /locus_tag="VFV93_13585"
     CDS             complement(729..1985)
                     /locus_tag="VFV93_13585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX5166428.1"
                     /translation="MHGRRRVVVTGIGAITPIGNGVDGLWNGVLTGQTAVRQLTRFDP
                     RMFRSQLAAEVTDFRPEDFFEPRRARRLDRFSQFAVAASQQALAASGVDPSRCPESIG
                     VYVGSALGGVSYGEEQHQVFIQNGVKAVSPMTALAVFGGAGPSNVAIELGLHGPALGN
                     ANSCASGTVAIGEAFRLLRDAPDAPQIMLAGGAEAPLSPLTYGAFAIIKAMSAANDQP
                     ERASRPFDRERDGFVMAEGSAMLVLEERRHALDRGARIYAEILGYGHTNDAHHMTAPL
                     PDGSQAARAISLALADGSVSPANVDYVNAHGSSTPLNDSTEVLAIRRALGQRAEEIPV
                     TGTKGLHGHALGASGAIEAAIACLAIQRGYVPGTANLRTPDPACDLRHLPGEGEARKI
                     GALLSNSFGFGGINACLVMSHPDWAG"
     gene            complement(2051..2557)
                     /locus_tag="VFV93_13590"
     CDS             complement(2051..2557)
                     /locus_tag="VFV93_13590"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5166429.1"
                     /translation="MNRSIEIRIDGDLDAIYRLGAEIEHWPQLLPHYRDVRVLWREGN
                     RCVAKMYASRDGIPVSWMCWQERDPETPTVTFRHIGGFTRGMVAVWTFDDHPDDTVTA
                     RITHEFRKGWPIQGLDRFVTDRIVRDFFTHSIAGKTLGMVKLLAEADRASRRAGQAIC
                     NRELAGDH"
     gene            complement(2587..3780)
                     /locus_tag="VFV93_13595"
     CDS             complement(2587..3780)
                     /locus_tag="VFV93_13595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5166430.1"
                     /translation="MIYDIVIAGGGPAGTSTAIGLAERGLRVALLDKARFPRVKPCAE
                     YVNPEAMRVLDRLGLLEPVRAAGAAVFRGMRVVSPGGQTVELDYSADACRHALGISRH
                     ALDLLAIERCRSLGVDVLEGARVRGVSTGDRVSSLSATQRGAAIEVRGWIVVGADGHH
                     SAVARLLGLDLPLRWPRRIGLAAHLDGFALTDEMGEMHAGRDGYCGIAPQEDGRVNAA
                     MVVDIARFGQRSGSVEAFFDAALASYPAIAERLRQAARATPVRGVGPLARRVRRTAGD
                     GYLLVGDAAGFFDPFTGEGIFDALHGGELAAEAIASALERGDTSAAGLAGYERARRTA
                     FADKRRAAWLVQAFVRSPRLMDYALQRVAARPTVRTTMTGVLGDYRDAGALLSPRFLW
                     ATLRP"
     gene            complement(3777..4484)
                     /locus_tag="VFV93_13600"
     CDS             complement(3777..4484)
                     /locus_tag="VFV93_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5166431.1"
                     /translation="MPLARRHQMELLDRLDAAACAGALSENLRDIRRANRWFGGTGAV
                     LAALSGLPSLWESGQPVSILDVATGSADIPVAVADWAERSGRDVTIVATDLQPAVLAV
                     ARAATHGERITIEQADALNLPYGDSAFDVVTLSLALHHFEPADAVRALHEMARVGRLA
                     LLVNDLERSRAGLAGAWLFAHLLTTNPMTRNDAPLSVRRAYTQPEALALANAAGWHSA
                     RVRPVVPFRYLLIGRPA"
     gene            complement(4542..5264)
                     /locus_tag="VFV93_13605"
     CDS             complement(4542..5264)
                     /locus_tag="VFV93_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5166432.1"
                     /translation="MTGAGPVQCPHCGTFMDENARFCPGCGTPRTSVREQLEREAAAT
                     GVPYATVLERARTSGSPVTAAPPGGWATSAPVARPQQEQGRGRLWLVLGIIGGILLLV
                     CAGCAVAGVVLMNRFDVNLGDSPEGDAARHQLQLAGAGRFEERWDLLHPDQQIAVPLE
                     LFTQCAEFDDVGSIEILGSFRDDDTFVPRAGIVDTRVVIFTYSDGGSTETDAVSMVKV
                     DGEWRWTMTSDQIADYQAGRCP"
     gene            5383..5847
                     /locus_tag="VFV93_13610"
     CDS             5383..5847
                     /locus_tag="VFV93_13610"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX5166433.1"
                     /translation="MGEAWRRIVASGAVDRPSPRCADPAPAGLIEGIQLFNAGEYFEC
                     HEVLEAIWREERDPIRYLYQGILQIGVGLHHLRNGNYRGATLLLHDGIDKTRRFTPRC
                     MGVDTLTLCDRAQECLDTLQTLGRERISDFDWSLVPVISGAWDADDTEPEIS"
     gene            6034..6747
                     /locus_tag="VFV93_13615"
     CDS             6034..6747
                     /locus_tag="VFV93_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166434.1"
                     /translation="MTVPGEQHRNQQLGENGARAAEAAEQIAISHAQTRELRERTGEL
                     VGELRAYVTYELGRRVRLGQIDPEDLLRDEVIDSVFAAAMTRLGQGQPIRDLRGYMRN
                     RAQETIQRESRRIQMERRQHVSLEQTLAPSGDTEDGEEVHIADVIPDPNAREPEDIVV
                     NNEMLQFLIDALADVPDLWRTVFLQRTMQERSAREVAEREELDIDDMRRITVRTREYL
                     RERFEGEYEFLDDLFDYDE"
     gene            complement(6763..6993)
                     /locus_tag="VFV93_13620"
     CDS             complement(6763..6993)
                     /locus_tag="VFV93_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166435.1"
                     /translation="MAMRRLLDFLDRLENEKIWYRLEHIRDSIMVESAIPGERWEIEF
                     FADGHVEVERFRSDGQIGGDEMLDVLFRDFSD"
     gene            complement(7011..8228)
                     /locus_tag="VFV93_13625"
     CDS             complement(7011..8228)
                     /locus_tag="VFV93_13625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5166436.1"
                     /translation="MSEKAEVVVIGGGIMGASVAFALTKLGVRDVLLVERETLAAGAS
                     GKTGALLRQHYTNVPEATLAHLSLQTYRHWAEEIGGDCGFAETGSIVTVATSGEDAVN
                     VERMRRVVAMQNQVGIKSEVITGDQLVELQPFATADDIAVAAYERESGYVDSLAATRG
                     MAEAAIRGGARFAEGRTVTGILTDGDRVRGVETDAGSVEAGAVVTAAGAWSVPLLAGI
                     GVEVPVEAQRVQVVILARPLAMPAVGTMTYVDTAAGFFCRNWGPNRTLAGVGGGEFHD
                     IVDPFNWDERPNQGFGDIVKRYISRRMPAMAEATTLYGHAGIYDMTPDTHPILSTVDG
                     LDGLYLMLGFSGAGFKKGPAIGQCMAELIVHGKATTVDLTPFRLARFDDDSWQEAWSP
                     DEYSFSTDFGHKF"
     gene            8344..9252
                     /locus_tag="VFV93_13630"
     CDS             8344..9252
                     /locus_tag="VFV93_13630"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX5166437.1"
                     /translation="MQTVSSRQHAFEFRPDIAPALRVRSGETVRFETSPEPAERLFAA
                     GADWCAQVDTRRLNAVTGPVHIDGVEPGDAVRVDILEIETLDWGWNAFFPNFGMLCEN
                     LTTPMLRRVPIEEGRCWITDALFVPLRPMIGCLGLAPERGASSTLAPPFPWAGNYDLL
                     QVKAGSTIWFPAQVKGGLFSLGDLHAAMGDGEATSVSIECAGAATVRLSADKQVDLQM
                     PRIVSDGRLFTVGLAERGNYTSARRHAADQLFNYLTVERRLTAEDAYTLFSAAGDLSF
                     GGPAGAVVLASIPLDVLDERDARARR"
     gene            complement(9302..11119)
                     /locus_tag="VFV93_13635"
     CDS             complement(9302..11119)
                     /locus_tag="VFV93_13635"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX5166438.1"
                     /translation="MLLTIMLLAGLAADTARPLTASAGEAAPSKSDLIGVNGNAFPWE
                     PHWNTFLSLLNQSNAGWARVELRWDYINPALGTWQWGLLDDQVNGYAGQGIHQLGLLD
                     YSVGWANGQGNAQTVFGPPTDLDAWEAYVRATVERYHDRVGAWEIWNEPDVAFFWNGQ
                     DGGDPAVYTELLRRAHRAIKSVDPNAVVMNGGVTGTERGASFLNRVLDLDGGEYLDAI
                     AIHGYVPNDGLDTSIYPEVIWPLISKVRERAGKPLWVTEFGWSSGCGAGGSVACSEAA
                     QANRIARHLPMLFSIGGVEHVLLFLFKDPGNQPNYFGLTDADGRTKPAYTAFATMAGR
                     LAGLRFERRVEMGDSSIWSMRFSNGDRTVDIIWSQTGSRQIWLPTASASVRTWKIDGS
                     SADVNASSGGVSLSIGLDPLIVERDGRALSSGASTCRYFPETNQSLCDSFLTFWERYG
                     GLMMFGYPISAATIENGKTVQYLERSKFEYQPEAAGTDWEVVGELLGRTITVGREHEQ
                     PFQRVSRYGTDGNCQFFDETGHGLCWGFRAYWQEHGGLWMFGYPISEEFEERNPDSGE
                     VYKVQYFERARFEWHPDNQGTPYAVLLGRLGAQLYDKRY"
BASE COUNT         1920 a         3822 c         3769 g         1907 t
ORIGIN      
        1 cgatgtcgtc cggatgggtg atgtcgtgga acgtcatcgc gatcagctcg cttggcgaat
       61 aacctagaat ccctgccatc gcgtcgttaa cggccagcca gcggccatcg agcgactgga
      121 ccgacatccc gatcgccggg ctctcgaaga cgctcgtcca ttgcccgttg agcaggtcga
      181 gattcgcgtc tgatcgcgca tcacgcatca cggcccgaag cagcaccggc cgcccggccg
      241 cgacctggca atccatcgcc ccactgaccg acagcggctc gccggttcga gtcagcagga
      301 cggtactcac gttgtgagcg gcccgttgct gaatgacgga gcgcatggtt tcagcgaagg
      361 agttctggtg attcgggtgg atcaggtcga gcagcaggat gcgagagatg tcctgatcgc
      421 cgtatccaag cgcacgacgc caggcactgt tgacgtagcg gaggaggcca ttgaccgacg
      481 cgacctgaat caggtccgag gagagttcgt tgagcgcggc gtggtcgatc gcatcgtctg
      541 ccgaccttcc atcacctggt tcagcactcg ccgtcatcat cacgctcgca cctcttgccg
      601 gaccggttga cgcctttccg aggacaggtt cctggaggtg agactccgcg acgacggagc
      661 cggtgaacgt cccagaatga gtcagtggcg taacgatacc agttgcagcg aggcaacgcg
      721 gctcttcttc aacccgccca gtcgggatga ctcatcacca ggcaggcgtt gatcccgccg
      781 aagccgaacg agttggaaag cagcgcaccg atcttgcgcg cttcgccttc tcccggcagg
      841 tggcgcaggt cgcaggcagg gtcgggcgtg cgcagattgg cggttcccgg cacgtagccg
      901 cgctggatcg ccaggcaggc gatcgccgct tcgattgcgc cgctcgcgcc gagtgcgtgg
      961 ccgtggagtc ctttggtgcc ggttactgga atctcctcgg cccgttggcc gagggctcgg
     1021 cggatcgcca gcacttcggt cgagtcgttg agcggcgttg aagatccgtg ggcattgacg
     1081 tagtcgacgt tcgccggcga gacgctaccg tccgcgagtg ccagtgagat cgcgcgggcc
     1141 gcctgcgagc cgtccggcaa cggcgccgtc atatgatggg cgtcgttggt gtggccgtag
     1201 ccgaggatct cagcgtagat ccgcgcgcca cggtcgagcg cgtgtcgccg ctcctccagg
     1261 accagcatcg cgctgccttc ggccatcacg aagccgtcac gctcgcggtc gaacggacgg
     1321 ctggcccgct ccggctggtc gttggcggca gacatcgcct tgatgatcgc gaacgcgccg
     1381 taggtgagtg gcgagagtgg cgcctccgcc ccgccagcca gcatgatttg cggtgcgtcc
     1441 ggggcgtcgc ggaggagccg gaaggcttcg ccgatagcga ccgtccccga cgcgcacgag
     1501 ttcgcgttgc cgagcgccgg gccgtgcagc ccaagctcga tcgcgacgtt gctgggaccg
     1561 gcgccgccga acaccgccag cgccgtcatc gggctgacgg ctttgacgcc gttctggatg
     1621 aagacctgat gctgctcctc gccgtacgag acgccaccga gggccgagcc gacgtagacg
     1681 ccgatcgatt cggggcatcg gctcgggtcg acgccgctgg cggccagcgc ctgctggctt
     1741 gctgcgacgg cgaactggga gaaacggtcc agccgccggg cgcgccgtgg ctcgaagaag
     1801 tcctcaggcc ggaagtccgt cacctcggcg gcgagctgcg agcggaacat gcgcgggtcg
     1861 aagcgtgtga gttgccggac ggcggtctgc cccgtcagga ctccgttcca gaggccgtcg
     1921 acgccgttcc cgatcggcgt gatggcaccg atcccggtga cgacgactct ccgtctgccg
     1981 tgcatgtggc gtcctctctc gtatctgatg ccggttgcaa ccgacgggct ctcgtgccga
     2041 accgctgcgc tcagtggtcc cctgcaagct cgcggttgca gatggcctgc ccggctcgcc
     2101 ggctggcgcg atctgcctcg gcgaggagct tgaccatccc cagggtcttg ccggcgatgc
     2161 tgtgggtgaa gaaatcgcgg acgatccggt cggtcacaaa acggtccagc ccctggatcg
     2221 gccagccctt gcggaactcg tgggtgatgc gggcggtgac ggtgtcgtcg ggatggtcgt
     2281 cgaatgtcca gacggcgacc attccgcgcg tgaagccgcc gatgtgacgg aaggtgaccg
     2341 tcggtgtctc ggggtcacgt tcctgccagc acatccacga gacggggatg ccgtcgcgcg
     2401 acgcgtacat cttcgcgacg cagcggttgc cctcgcgcca gaggacgcgg acgtcgcggt
     2461 agtgcggcag gagttgcggc cagtgctcga tctcggcgcc gagccggtag atcgcgtcca
     2521 ggtcgccgtc tatccgaatc tcgatactgc ggttcatcgt cggttatctc cagcattagt
     2581 gcgcgttcag ggccgcagcg tggcccacaa gaaacgcggc gacagcagcg ccccggcgtc
     2641 gcgatagtcg ccgaggacgc cggtcatcgt cgtccggacc gtcggccgtg ccgcgacccg
     2701 ttgcagtgca tagtccatca gacgcggcga gcggacgaat gcctgcacca gccaggcggc
     2761 ccgccgcttg tcggcgaagg ccgttcgccg cgcccgctcg tagccggcca gaccagcagc
     2821 cgacgtatcc ccgcgctcaa gcgcggacgc gattgcctcc gccgccaact ctccaccatg
     2881 cagcgcatca aagatgcctt cgccggtgaa cgggtcgaag aagccggccg catcgccaac
     2941 cagcaagtag ccatcaccgg ctgtacgacg cacgcggcgg gccagcgggc cgacgccgcg
     3001 caccggtgtc gcccgtgctg cctggcgcag acgctccgcg atggccgggt aggatgccag
     3061 cgccgcgtcg aagaacgcct cgaccgatcc actgcgctgc ccgaagcgtg ctatgtcaac
     3121 gaccatcgcg gcgttgacgc ggccgtcctc ctggggcgcg atgccgcagt agccatcgcg
     3181 gccggcgtgc atctcgccca tctcatcggt gagcgcgaat ccgtcgaggt gggccgccag
     3241 cccgatccgg cgtggccagc gcagtggcag gtcgagcccc aacagccgcg cgaccgccga
     3301 gtggtgtcca tcggcgccga cgacgatcca gccacgaacc tcgatcgccg cgccacgctg
     3361 cgtcgccgac aacgagctga cccgatcgcc cgtcgacacg ccgcgcaccc gtgccccttc
     3421 gagcacatcg acaccgagcg agcggcagcg ttcgatcgcc agcagatcca gcgcatgacg
     3481 cgagatcccc agtgcgtgac ggcaggcgtc ggcactgtag tcgagctcga ccgtctgccc
     3541 gcctggcgag acgactcgca tgccccggaa aacggccgcg ccggctgcgc ggaccggctc
     3601 cagcagcccg agtcggtcga ggacacgcat cgcctccggg ttgacgtact cggcgcaggg
     3661 cttgacgcgg ggaaagcgcg ccttgtcgag cagcgcgacc cgcaggccgc gctcggccag
     3721 cccgatcgcc gtgctggtac cagccggccc gccgcccgcg atgacgatgt cgtagatcat
     3781 gccggcctgc cgatcaacag gtagcggaac gggacgactg gccgcacccg tgcgctgtgc
     3841 cagccggccg cgttcgccaa cgctaatgcc tcaggctgcg tataggctcg ccggaccgat
     3901 agtggcgcgt cgttgcgggt catcggattg gttgtcagca gatgcgcaaa gagccaggcg
     3961 ccggccagac cggctcggga gcgctccagg tcgttgacca gcagcgccag gcggccgacg
     4021 cgagccatct catgcagggc gcgcaccgcg tcagccggct caaagtggtg cagcgccagc
     4081 gacagcgtga cgacgtcgaa cgcgctgtcc ccgtagggca ggttcagcgc gtcggcttgc
     4141 tcgatcgtga tccgttcccc atgggtcgct gcccgcgcga ccgcgagaac ggccggctga
     4201 agatcagtcg caacgatcgt tacgtctcga ccgctgcgct cggcccagtc cgcaaccgcg
     4261 acgggtatgt cggccgaacc agttgcgaca tccaggatcg acaccggctg gccggattcc
     4321 cagagcgacg gcaggcccga caatgcagcg agaaccgctc cggtgccacc gaaccagcga
     4381 ttcgcgcgcc gaatgtcgcg caggttttca gacagggcgc cggcgcacgc agcggcatcc
     4441 aggcgatcca gcaactccat ctgatgcctt cgcgcaagtg gcacgatctc gcctccccgg
     4501 cgatggtgtg attgcctgac acctgaatgc ccggctcgac actacgggca gcggccggcc
     4561 tgatagtcgg cgatctggtc cgaggtcatc gtccagcgcc actccccatc gaccttcacc
     4621 atactgactg catcggtctc cgtcgatcct ccgtcggaat aggtgaagat cacgacccgt
     4681 gtatcgacga tgccggcgcg cggcacgaag gtgtcgtcgt cgcggaagct gccgaggatc
     4741 tcgatcgagc cgacgtcatc gaactcggcg cactgtgtga acagctccag cgggacagcg
     4801 atctgctgat cggggtgcag cagatcccag cgttcttcga atcgccccgc gccagcgagc
     4861 tggagctggt ggcgggcagc gtccccctcg ggactgtcac ccaggttgac gtcgaagcga
     4921 ttcatcagaa cgacgccggc gacggcgcag ccggcacaga ccagcagcag gatgccgccg
     4981 atgatgccca ggaccagcca gagccgcccg cgtccctgct cctgctgcgg ccttgcgacc
     5041 ggcgcggaag tggcccagcc gcccggtggc gcggctgtga ccgggctgcc ggacgttcgc
     5101 gcgcgttcga ggacggttgc gtacggaaca ccggtcgcgg ccgcttcgcg ctcaagctgc
     5161 tcgcgaacgg atgtccttgg cgtcccacag ccggggcaga atcgcgcgtt ttcgtccatg
     5221 aaggtgccgc agtgggggca ctgaactggt cccgctcccg tcatcggcgt cctcgcttcg
     5281 gtggttcgtg gcgtgggcag atgccggcgt tcggtctatg ccggctagcg gcacggtacc
     5341 atatctggac gctggttcgg tcggtcacgg gcgggaggac agatgggcga ggcatggagg
     5401 cggatcgtcg cgtcgggcgc ggttgaccgc ccaagcccgc gctgcgccga tccagcgccg
     5461 gctgggttga tcgagggcat tcaactcttc aacgccggcg agtacttcga atgccacgag
     5521 gtgctcgaag cgatctggcg cgaggaacgc gacccgatcc gctatctcta tcagggcatc
     5581 ctccagatcg gtgtcggcct ccaccatcta cgcaacggca actaccgcgg ggcgacactc
     5641 ctgcttcacg atggcatcga caagacgcgc cgcttcaccc cgcgctgcat gggcgttgac
     5701 acgctcaccc tctgcgaccg cgcccaagaa tgcctcgaca ccctccagac cctcggccgc
     5761 gagcggatct ccgacttcga ctggtcgctc gtgcctgtca tcagcggggc gtgggatgct
     5821 gatgacacag aacctgagat cagctagaac gttcccagag gcgcggcggc ctctcgtata
     5881 ttgatccccg acacggcttc ccccgcgcct gtagaatatc ctccgcccgt gcgtccaatg
     5941 gtcgctctgg tctacacttt tatgatcggt caccagcgca gttcgctggt gccggtcgct
     6001 cgatccggcc agcacatccg aacctgaggt ggaatgacag tccctggaga acagcaccgc
     6061 aaccagcagc ttggcgagaa cggtgcccgt gccgcagagg cggccgagca gatcgcgatc
     6121 agtcacgccc agacgcgtga gctccgcgag cgaacgggcg aactggttgg cgagttgcgt
     6181 gcctatgtca cgtacgagct tggccgtcgc gtccggctgg gccagatcga tcccgaggac
     6241 ctgctgcgcg acgaggtgat tgacagcgtc ttcgccgctg cgatgacgcg cctcggtcag
     6301 gggcagccca tccgcgacct gcgcggctac atgcgcaacc gtgcccagga gacgatccag
     6361 cgcgagtcac gccggatcca gatggagcgc cgccagcacg tctcgctcga acagacgctg
     6421 gcgccatccg gcgataccga ggacggcgag gaagtccaca tcgccgacgt gatccccgat
     6481 ccgaacgccc gcgagccgga ggacatcgtc gtcaacaacg agatgctcca gttcctgatc
     6541 gacgcgctgg ccgacgtgcc cgacctctgg cgaacggtct tcctccagcg aacgatgcag
     6601 gagcgcagcg cccgcgaggt ggccgaacgc gaagagcttg acatcgacga catgcgccgc
     6661 atcaccgtcc gaacccgcga gtacctgcgc gagcgcttcg aaggcgagta cgagttcctc
     6721 gacgacctct tcgactacga cgagtgatgc gtgtgcacgc gtctagtcgc tgaagtcacg
     6781 gaacagcacg tcaagcatct catcgccgcc gatttggccg tcgctccgga atcgttcgac
     6841 ttcgacgtgg ccatcggcga agaactcaat ctcccatcgc tcgcccggaa tggcggattc
     6901 caccattatc gaatcgcgga tgtgttcaag gcgataccag atcttctcgt tctccaaccg
     6961 atcgagaaaa tccaacaacc tgcgcatcgc caacgaagaa tcagtgccca tcagaactta
     7021 tgtccgaagt cggtcgagaa tgagtattcg tctggactcc acgcttcctg ccaactgtcg
     7081 tcgtcaaagc gggcgaggcg gaacggggtg aggtcgacgg tcgttgcctt gccgtggacg
     7141 atcagttctg ccatgcactg gccgatggcg gggcctttct tgaagccggc gccggagaat
     7201 cccagcatca ggtagaggcc atcgagaccg tcgacagttg agagaatcgg atgcgtgtcg
     7261 ggtgtcatgt cgtagatacc ggcgtggccg tagagggtag tcgcctcggc catcgccggc
     7321 attcgacggg agatgtagcg cttgacgatg tcgccgaaac cctggttggg ccgctcgtcc
     7381 cagttgaacg ggtcgacaat gtcgtggaac tcgccgccgc cgacaccggc cagcgtccgg
     7441 ttcggccccc agttgcgaca gaagaagccg gcggccgtgt cgacgtaggt catcgttcca
     7501 actgccggca tcgccagtgg ccgcgccagg atgacgacct gcacccgctg cgcctcgacc
     7561 ggcacctcga cgccgatccc ggccagcagc ggcacggacc aggcgccggc cgccgtcacg
     7621 accgcgccgg cttcgaccga acctgcatcc gtctcgacgc cgcgcaccct gtcgccgtcc
     7681 gtgagaatgc cggtcacggt tcgtccctcg gcgaagcgcg caccgccacg gatggcagcc
     7741 tcggccatcc cgcgtgttgc ggccagcgaa tcgacgtagc cggattcgcg ctcgtaggcg
     7801 gcgacggcga tgtcgtctgc cgtcgcgaag ggttgtagct cgacgagctg atcgccggtg
     7861 atgacctccg acttgatccc gacctggttc tgcatagcga cgacccgccg catgcgctcg
     7921 acgttgacgg cgtcctcgcc gctggtcgcg acggtcacga tcgagccggt ctcggcgaag
     7981 ccgcagtcgc cgccgatctc ctcggcccaa tggcggtagg tctggagcga gaggtgggcg
     8041 agtgtcgcct cggggacgtt ggtgtagtgc tgtcgcagca gcgcgcctgt cttgccgctg
     8101 gcgccggctg ccagcgtctc gcgctcgacg agcagcacat cgcgaacgcc gagcttcgtc
     8161 agcgcgaacg cgacgctcgc ccccatgatc ccgccgccga tgacgacgac ctccgccttc
     8221 tcgctcacct gccgcctcgc ttcctgcctc cgaaccgcct gccgcgcgcc gatcgtagca
     8281 ccacgggctg gttggcgtgc catactcaag ccggtttgag aacgtcggtt gggagagggc
     8341 aggatgcaga cagtcagtag caggcagcac gcgttcgagt tccggcccga tatcgcgccg
     8401 gcgttgcggg tgcgatcggg cgagacggtc cgcttcgaaa cttctccaga gccggccgag
     8461 cggctcttcg cggccggtgc cgactggtgc gcgcaggtcg acactcgacg gctgaacgcc
     8521 gtgaccggac cggtccacat cgacggtgtc gagccgggag acgcggtccg tgtcgacatt
     8581 ctggagatcg agacgctcga ctggggctgg aacgcattct tccccaactt cgggatgctc
     8641 tgcgagaacc tgacgacgcc gatgctgcgg cgtgtgccga tcgaggaggg tcgctgctgg
     8701 atcaccgacg cgctgttcgt cccgctgcgg ccgatgatcg gctgtctcgg cctcgcaccg
     8761 gagcggggag cgtcatcgac gctagcgccg ccgtttccgt gggcaggcaa ctacgatctt
     8821 ctgcaggtga aggccggcag cacaatctgg tttcccgcgc aggtcaaagg cgggttgttc
     8881 tcgctcggcg atctgcatgc cgcgatgggc gacggtgagg caaccagcgt ctcgatcgag
     8941 tgtgccggag ccgcaaccgt ccggctcagc gctgacaagc aagtcgacct gcagatgcct
     9001 cggatcgtgt cggacggtcg gctcttcacg gtcgggctgg ccgagcgagg caactacacc
     9061 tctgcccgtc gccacgcggc cgatcagttg ttcaactacc tgacggtcga gcgacggttg
     9121 acggccgaag atgcctacac gctcttctcg gctgccggcg acctgtcatt cggcgggcca
     9181 gccggtgccg tcgtcctggc cagcatcccg ctcgacgtgc tggatgagcg ggacgccagg
     9241 gcgcgacgat gatgatcctg tcgtcctgaa ctgcggctca ggacgacagt ctgacgccgg
     9301 tctaatatcg cttgtcatag agttgtgcgc cgagccggcc aaggaggacg gcgtagggcg
     9361 tgccctggtt gtccgggtgc cactcgaagc gggcgcgctc gaagtactgg accttgtaga
     9421 cctcgccgga gtccgggttg cgttcttcga actcctcgga gatcgggtag ccgaacatcc
     9481 agagtccgcc gtgctcctgc cagtaggcgc ggaagcccca gcagaggccg tggccggtct
     9541 cgtcgaagaa ttgacagttg ccgtctgtac cgtagcgcga gacacgctgg aatggctgct
     9601 cgtgctcgcg gccgaccgtg atcgttcgtc caagcagttc gccaacgacc tcccagtcgg
     9661 tgccggccgc ttccggctgg tactcgaact tcgagcgttc caggtactgg accgtcttgc
     9721 cattctcgat cgtcgccgcc gagatcgggt agccgaacat catcagcccg ccgtagcgtt
     9781 cccagaaggt caggaagctg tcgcagagcg attgattcgt ctcgggaaag tagcggcagg
     9841 tgctggcgcc gctggagagc gcccggccgt cgcgttcgac gatcagcggg tccagcccga
     9901 tcgacagcga gacgccgccg gaggacgcgt tgacatcggc gctgctgccg tcgatcttcc
     9961 aagtgcgcac gctggcgctg gcggtcggca gccagatctg gcggctgccg gtctgcgacc
    10021 agatgatgtc gacggttcgg tcgccgttag agaagcgcat cgaccagatg ctggagtcac
    10081 ccatctcgac ccggcgctcg aagcgcagcc cggccagccg gccggccatc gtcgcgaacg
    10141 ccgtgtaggc tggcttcgtt cgcccgtcgg cgtcggtcag cccgaagtag ttcggctggt
    10201 tgccggggtc tttgaacagg aacagcagaa cgtgctcgac gccgccgatc gaaaagagca
    10261 tcggcaggtg acgggcgatt cgattggcct gagccgcttc cgagcaggcg acagagccgc
    10321 cagcgccgca accgctcgac cagccaaact cggtcaccca aagcggcttg ccggcccgct
    10381 cgcggacttt gctgatcagc ggccagatga cctcggggta gatcgaggta tcgaggccgt
    10441 cgttcggaac gtagccgtgg atcgctatgg cgtcgagata ttcgccgccg tcgaggtcga
    10501 ggacgcggtt gaggaaactg gctccgcgct cggtgccggt gacgccgccg ttcatcacga
    10561 ccgcgttggg gtcaacagac ttgatggcgc gatgggcgcg gcgcagcaac tcggtgtaga
    10621 cggctggatc gccgccgtcc tgaccgttcc agaagaaggc cacatccggt tcgttccaga
    10681 tctcccaggc cccgacccgg tcgtggtaac gctcgactgt tgcccgaacg taggcttccc
    10741 aggcgtccag atcggttggc ggcccgaaga cggtctgcgc gttgccctgg ccgttggccc
    10801 agccgaccga gtagtccagc aggccaagct ggtggatgcc ctgcccggcg tagccgttga
    10861 cctgatcgtc cagcaacccc cattgccacg tccccagtgc ggggttgatg tagtcccagc
    10921 gcaactcgac tcgcgcccaa ccggcgttgc tctgattcaa cagcgagagg aacgtgttcc
    10981 agtgtggctc ccatgggaac gcgttcccgt tgacgccgat caggtcggac ttcgacggtg
    11041 cagcttcgcc ggcagacgcc gtcagcgggc gggcagtatc ggcagccagg ccggccagca
    11101 gcatgatcgt gagcagcaac gaactccagc gcacggcaaa atcgcgcccc acgcgcttca
    11161 acgacacttc caattcaact gtccacttcc cggaatctgg acacagccgg acactgtcag
    11221 accacgatcg tggccgacag tcgcgcggca tccacccatt gccgtttcgc tcagtaccgt
    11281 aagtatgctt gggtggcctt gctcgccgta tcacccaaac gtcacggaga cacgcggggc
    11341 actcgcggta ccgaatggga ccagtcgcgg gacgcggtca gttcgtgagg aagcgtgcct
    11401 tatccctgtc gtcctgag
//
LOCUS       DASUGJ010000600         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1209595, whole genome shotgun sequence.
ACCESSION   DASUGJ010000600 DASUGJ010000000
VERSION     DASUGJ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209595"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..790
                     /locus_tag="VFV93_13640"
     CDS             86..790
                     /locus_tag="VFV93_13640"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5166439.1"
                     /translation="MVTTTLWLVRHGETSSNADGVFQGHLDVGLNQRGEEQAAAVGER
                     LACIDFDAVYASDLERAARTAEIIVNGRCPVVLDPDLREMHYGVLQGVRYHDAPSVLA
                     SHGMSEAWNSGEFHRKGLASPGGESLRQFRHRSRRFLARVDRLHPADAGHNVLIVAHG
                     GKLSVLMTVLLEIPSISRRSFRFANCGITRVSRSGLRTTLDLHNLVVWDDTLLVSDHA
                     QSGRLLDPGESHLPRT"
     gene            complement(726..1910)
                     /locus_tag="VFV93_13645"
     CDS             complement(726..1910)
                     /locus_tag="VFV93_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5166440.1"
                     /translation="MSAGYDLERARANLPVLRDWTYLNTGTVGVMAEPVLRKHLEQIA
                     DHERFGHATQARAIEGYERARRTLAMLTGSNPTDIALNRNATDGINWVAATFPLGDGD
                     EVITSTEEHPAMVYPWLAACERAGARLRFIDLHTSPDALRSALETVVSPATRVVALSQ
                     VSCETGKRVPVEIVRDVVGPEVAILVDASQSVGQFPIDIPALRANFVIGNGHKWLAGP
                     KGSGFAWFDADSLDLVPPVYFGDGAVDPRWSRSYYQADPSPHLRFGGNASRFEYGTRA
                     WHMYEGLADAIEYQAGFGWDAIFDHVDRMSSLAKAVLEEIPGVDVITPTSWQESSGIV
                     SFTIMGMAGEDVSRELWENERITQRRVEVPSAVRVSCTYFTSEEDVTRLGQAVDRIAR
                     GR"
BASE COUNT          369 a          687 c          702 g          390 t
ORIGIN      
        1 gagatcacgg tcaagtaggt ccgttcattc gtcgatcgca acggggcggc cggttggccg
       61 ccctttcgtc tggaaatggc aggtcgtggt gacgacaaca ctctggctcg tccggcatgg
      121 cgagacgagc tcgaacgcgg atggggtctt tcagggacat ctcgacgttg ggctgaacca
      181 gcgcggcgaa gagcaggctg cggccgtcgg cgaacggctg gcctgtatcg acttcgacgc
      241 tgtctatgcc agcgacctgg agcgggccgc ccggacagcc gagatcatcg tcaacggccg
      301 ctgtcccgtg gtgctcgatc ccgacctgcg cgagatgcac tacggcgtct tacagggtgt
      361 tcgctaccac gatgcgccgt cggttcttgc atcgcatggc atgagcgagg cgtggaacag
      421 cggcgagttt catcgcaagg gtctggcttc gccgggcggc gagagcctgc gccagttccg
      481 acatcgctcg cggcggttcc tcgcgcgtgt cgaccggctc catccagcgg atgccgggca
      541 caatgtcctc atcgtcgccc acggtggcaa gctgagcgtg ttgatgacgg tgctactgga
      601 gatcccctcg atcagccgcc gttcgttccg cttcgcgaat tgcggcatca cgcgtgtgtc
      661 acgatccggc ctacgaacga cgctcgacct gcacaatctc gtcgtctggg acgataccct
      721 gctggtcagc gaccacgcgc aatccggtcg actgcttgac ccaggcgagt cacatcttcc
      781 tcggacgtga agtaggtgca cgagacgcgg acggcgctcg gcacctcaac ccgtcgctgg
      841 gtgatgcgct cattctccca aagctcgcgg ctgacgtcct cgccggccat ccccatgatt
      901 gtgaacgaga cgatgccgga cgactcctgc cacgacgtgg gcgtgatgac atcgacgccg
      961 gggatctcct ccagaacggc cttcgccagc gacgacattc gatcgacgtg gtcgaagata
     1021 gcgtcccagc cgaagcctgc ctgatactcg atcgcgtcgg ccaacccctc gtacatgtgc
     1081 caggcgcgcg tgccgtactc gaagcggctc gcattgccgc cgaagcgcag gtggggcgac
     1141 gggtcggcct ggtagtaact gcgcgaccag cgcggatcga cggcgccatc accgaagtag
     1201 accggcggga cgaggtcgag cgaatctgcg tcgaaccagg cgaagccgga tcccttcggc
     1261 cccgccagcc acttgtgtcc gttgccgatc acgaagttgg cgcgcaacgc tggaatgtcg
     1321 atcggaaact gaccgaccga ctgcgaggcg tcgacgagga tcgccacctc cgggccaacc
     1381 acgtcacgga cgatctcgac agggacgcgc ttgcccgtct cacacgagac ctggctgagt
     1441 gctacgacac gcgtggccgg cgagaccact gtctccagcg cggatcgcag cgcgtccgga
     1501 ctggtgtgga gatcgatgaa gcgcagtcga gccccggccc tctcgcaggc ggccagccac
     1561 ggatagacca tcgccgggtg ctcttcagtt gatgtgatga cctcatcgcc gtcgccgagc
     1621 gggaaggtgg cggcgaccca attgatgcca tcggtcgcgt tgcgattgag agcgatatca
     1681 gtggggttgg aaccggtcag catcgcgagc gtccgccggg cgcgctcgta gccctcaatt
     1741 gcccgcgcct gggtcgcgtg tccgaatcgc tcatggtcgg cgatctgctc caggtgcttc
     1801 cgcagcaccg gttcggccat gacaccgacc gtgccggtat tgaggtaggt ccagtcgcgc
     1861 agtaccggca ggttcgcccg tgctcgttcc aggtcgtatc cagcgctcat cgttgtctcc
     1921 atcagttgcg atgtccgttc gagtgcggac ggtaacagtg ctgacgagac gcgtcaacag
     1981 acgcgaaagc ggccggggtt tggacccggc cgctaagtgc tcgtgccgat gagattgtga
     2041 cgggttgtca cgccgccatt cggtcgtaga gttcctcgta ctcacagaga agctggagcc
     2101 agacgtcttc ccagcggcgg acatcgtcgc cgttgacggc tgcctggg
//
LOCUS       DASUGJ010000601         5024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1627939, whole genome shotgun sequence.
ACCESSION   DASUGJ010000601 DASUGJ010000000
VERSION     DASUGJ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627939"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFV93_13650"
     CDS             <1..593
                     /locus_tag="VFV93_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5166441.1"
                     /translation="SHLLNRDITLDTYVTDIVQVLFYEDLTDVILVGHSFGGTVITGV
                     AERASERIAQLVYLDAEVPRDGESDAGCLTPNMEEFVWQRARERGDGWLFTPSPVASF
                     GVSDPADQAWADARMTAQPAATLTQPVRYGAAVEALPRHFIACRPYEPLIADIRARVQ
                     QEPGWTYQEIDGGHIVMLTRPHELAECLLGFAAAPA"
     gene            709..2412
                     /locus_tag="VFV93_13655"
     CDS             709..2412
                     /locus_tag="VFV93_13655"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX5166442.1"
                     /translation="MTAIDSTTVPAPPVGTSHAAADFLVTWEQPGDDQRFWTHERWGI
                     AVPITPLSFAFTDLIYGGMNAGAAAYSLPTRVAARRINTFLYMSEDPSSPTADAADDG
                     QRAEQLLNTALTTLGERWDADWLPEVQRHLAYWEGYDLAAAPLPALRHHLDDTIGRLQ
                     RLWEIHFLIIFPMLLAISEFDELYRELMGGEDSFDSYRLLQGLDNRTVDSGRALWDLS
                     RRALAAPPVRSALRDSAPAGVLADLEASPEGVAFLAELRPYLAEYGQRSNNPLELGES
                     SWIEDPTPVLATLREYIDQPDRDVAAERAELVAERERAIAATRSRLTGYPRPVVEQFE
                     LLLKGAQVATVLSEDHAFWIDLCGLYQARRVAVEIGRRLADAQAIPDAGDVFYLTLDE
                     VREALDGRPARNAPALVASRKAEMEYYRTITPPPAIGSAPPESQADDPLSRAMAKFMG
                     APPPVATEPGVVLGIAGSPGIVRGTAKVVLTLADAGKLQPGDILVTEYTGPWWTPLFG
                     IAGALVTDFGGVLSHCAIVAREFGIPAVVGAGSATTTLLDGQLIEVDGDQGIVRVIAN
                     P"
     gene            2417..3835
                     /locus_tag="VFV93_13660"
     CDS             2417..3835
                     /locus_tag="VFV93_13660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166443.1"
                     /translation="MTARDAPAAERVGLVTVALGTMLAPLNSTMIVVALPTILREFEY
                     TLAWGAWIVVAYLVTMAAGQPLGGSLGDRYGQRRLFQIGLVGFLAASALATLAPSLAV
                     LIVARTCQAAAGALVVPNGMALVRSLVAAERHGAAFGRIGAGVAIAATIGTPLGGVLT
                     DAFDWRAIFAANLALIGPALVLSVRLPAGRTAAQVGRFDLSGAALLITTLITLAASLT
                     LWRVAQAPVVLAPLLGLTTIVAGLLLWRHVSDHPAPVVRLNLFRQSGYAPASLLALLV
                     NLTMYTVLLSLPVFLTERAGWSSSATGVLLAAMSLQMVVFAPFGGWLADRRGWRYPAT
                     LGSVLLAAGALALVSIDTGWGWAVYLGPLVLIGAGVGLSSAPTQALAVDAAPAAAAGQ
                     AAGLFSTTTYLGSILGSAGMAAILGDGVPTTASFRLLYVAVSIAAILGVLAAVRLPAG
                     HGRRVDEQPDQAAARDLAPSRR"
     gene            4212..>5024
                     /locus_tag="VFV93_13665"
     CDS             4212..>5024
                     /locus_tag="VFV93_13665"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5166444.1"
                     /translation="MDRVSPGSHSGLVGRDRELAILNGCLTAAQGGRGSLVLISGEAG
                     IGKTALTNSFTHAATNARLTVLTGHCYDRTETPPYGPWREIGQRVDAIPVPRIDDATS
                     QADLFTQARDFLAAMTAERPLVLVLEDLHWADNASLDLLRFLARGFGDLPLLCVATYR
                     DDEVERHHPLAGLVPLLVREAPTERLSLRPLNTSAAQALVRARYDFAEPASERLAMYL
                     IERTEGNALFMTELLRSLEEDRLLERVVSDPHAWSLAPTPVPSLLQQIVDDRL"
BASE COUNT          743 a         1711 c         1687 g          883 t
ORIGIN      
        1 gcagccacct gctcaaccgc gatataacgc tcgacaccta tgtcaccgac atcgtgcagg
       61 tcctatttta tgaagatctg acggatgtga tcctcgtcgg tcacagcttc ggcggcacgg
      121 tgatcaccgg cgttgccgag cgcgcgagcg agcggatcgc gcagttggtc tatctcgacg
      181 ccgaggttcc gcgcgacggc gaatcggacg ccggctgcct gacaccgaac atggaggagt
      241 ttgtgtggca acgcgccagg gaacgcggcg atggctggct gttcacgccg tcacccgtgg
      301 cctcgtttgg cgtgagtgac ccagccgatc aggcctgggc tgatgcgcgc atgacagccc
      361 agcccgccgc aaccctcacg cagccggtgc gctatggcgc ggcagtggaa gcgctgcctc
      421 ggcacttcat cgcctgccgc ccgtacgagc cactgatcgc ggatattcgc gcgcgggtcc
      481 agcaggaacc gggttggacc tatcaggaga tcgacggcgg tcacattgtg atgctcacgc
      541 gcccgcacga gctggccgag tgcctgttgg ggttcgcggc agcgcccgct tgaccgccca
      601 ggcatgccag actctcaccg ccgattcgtc tgcccatccc ataccgggac cctatgaacg
      661 acacacgctc accgtcctgc tcggcatatc ttcgaatggg agagacgcat gacagccatc
      721 gattcgacaa ccgtgccagc gccgccggta ggaacctcac acgccgcagc cgactttctg
      781 gtgacctggg agcagcccgg cgacgatcag cgcttctgga cgcacgagcg ctggggcatc
      841 gcggttccga tcacgccgtt gtccttcgcg tttaccgacc tcatctatgg cggcatgaat
      901 gccggagcag cagcctactc gctgccaacc cgcgtggctg cgcgccgtat caacacgttc
      961 ctctacatgt cggaagaccc aagctcgcca acggcagacg cggctgacga cggccagcgc
     1021 gccgagcagc tcctgaatac ggcgctgacg acgctcgggg agcgctggga cgccgactgg
     1081 ctgcctgaag tgcagcgcca tctcgcctac tgggaaggct acgacctggc tgcggcgccg
     1141 ctgcctgcgc tccgccatca tctggatgac acgattggcc gtttgcaacg cctgtgggag
     1201 atccacttcc tgatcatctt cccaatgctg ctcgccatca gcgagttcga cgagttgtac
     1261 cgggaactga tggggggcga ggattcgttc gactcgtacc ggctcctcca ggggcttgac
     1321 aaccggacag tcgacagcgg tcgcgcgctt tgggacctga gccggcgggc gctcgctgcg
     1381 ccgccggtgc ggagcgcctt gcgagatagc gcgccggccg gtgtgctggc tgacctggag
     1441 gcgtctccgg agggcgtggc cttcctcgcc gaactgcgac catatctcgc ggaatacggg
     1501 cagcgttcca acaatcctct cgaactcggc gagtcgagct ggatcgagga tccgacgccg
     1561 gtactcgcca ccctgcggga gtacatcgat cagccggacc gtgatgttgc ggccgagcgg
     1621 gccgaactgg ttgctgagcg cgagcgcgct atcgcggcga cgcgcagccg tctgaccggc
     1681 taccctcggc cggttgtcga gcagttcgaa ttgctgctca agggtgccca ggtagcgacc
     1741 gtcctctccg aggaccacgc gttctggatc gacctctgcg gtctatacca ggcgcggcgg
     1801 gtcgccgtcg agattgggcg ccggctggcg gatgcccagg cgatccctga cgccggcgat
     1861 gtcttctatc tgacgctgga cgaagttcgc gaggcgctgg acggtcgtcc ggcccgcaac
     1921 gcgcccgcgc tggtcgcgtc ccgcaaggcc gagatggagt actaccgcac gatcacgccc
     1981 ccgcctgcga tcggcagcgc gccgcctgaa tcccaggccg acgatccgct cagccgcgcg
     2041 atggcgaagt tcatgggcgc gccgcctccg gtagcgacgg agcctggggt cgtgctgggc
     2101 atcgccggct caccgggtat cgtgcgcggc acggccaagg tggtgctcac actcgccgat
     2161 gccggcaagc tgcagccggg agacatcctg gtgaccgagt acaccgggcc gtggtggacg
     2221 ccgctgtttg ggatcgcggg ggcgctcgtc accgacttcg gcggtgtcct gagccattgc
     2281 gcgatcgtgg cgcgcgagtt cggcatcccg gccgtcgtcg gggctggatc ggcgacgacc
     2341 acgctcctcg acggccagct gatcgaggtc gatggcgatc aggggatcgt ccgggtcatc
     2401 gcgaacccat aagccaatga cagccagaga cgccccagct gctgagcggg tcggattggt
     2461 caccgttgcg ctcggcacga tgctggcgcc gctcaactca acgatgatcg ttgtcgccct
     2521 gccgacgatc ctgcgtgagt tcgagtacac gctggcctgg ggcgcctgga tcgtcgtcgc
     2581 gtacctggtg acgatggccg ctggccagcc gctcggaggc agcctcggcg accgctacgg
     2641 ccagcgccgg ctgttccaga tcgggctggt cggcttcctg gccgcctcgg cgctcgcgac
     2701 gctggctccc tcgctggcgg tgctgatcgt ggcccgcacc tgccaggcgg cggcaggtgc
     2761 gctggtcgtt cccaacggca tggcgctggt gcgatcgctg gtcgcggccg agcgtcacgg
     2821 cgcggccttt gggcgaatcg gcgctggtgt cgccatcgcg gccacgatcg ggacgccgtt
     2881 gggtggtgtg ctgacggacg cattcgactg gcgtgcgatc ttcgccgcga acctcgccct
     2941 gatcgggccg gcgctcgtcc tcagcgtccg gctgcctgcc ggaaggacag cggcgcaggt
     3001 cggacgcttc gatctatccg gcgcggcgct gctgatcacg acgctcatca cgctggcggc
     3061 ctcgctgacg ctctggcgtg tggcgcaggc gccggttgtg ctggccccgc tgctcgggct
     3121 gacgacgatc gtcgccggcc tgctgctctg gcggcacgtg tccgatcacc cggcgccggt
     3181 cgtacggctc aacctgttcc gccagtccgg ctatgcgccg gcgtcgctgt tggcgctact
     3241 cgtcaacctg acgatgtata ccgtgctgct ctcgctcccg gtctttctca cggagcgcgc
     3301 cggctggagc agcagtgcga ccggcgtcct gctggcggcg atgtcacttc agatggtcgt
     3361 cttcgcgccg ttcggcggct ggctcgccga tcgtcgcggc tggcgctacc ccgcgacact
     3421 cggatcggtg ctgctggccg ccggcgcgtt ggcgctggtg tcgatcgaca cgggctgggg
     3481 ttgggcggtc tacctcggcc cgctcgtctt gatcggggcc ggcgtcgggc tgtcgtcagc
     3541 accgacccag gcgctggcgg ttgacgccgc tcccgccgcc gccgccggcc aggcggccgg
     3601 actgttctcg acaaccacct atctcggcag catccttggc tccgccggca tggccgccat
     3661 cctgggcgat ggcgtgccaa ccaccgccag cttccgcctc ctgtatgtcg cggtctcgat
     3721 cgcggcgatc ctcggagtgc tggcagccgt tcggttgccg gcgggacacg gccggcgagt
     3781 cgacgagcaa cccgaccagg cagcagcacg agatctcgcg cccagccggc gttgatgcgg
     3841 ctggaagggg tccagcgaga cgtaccggcc tgattggccg cgacgccgat atcgctgctg
     3901 tccatcaacg gctgctgcgc gatgatatcc acaggagacc ttaccatccg cgaccaggcg
     3961 cgaccggtcg tcctggcggt cgagtatcag ggacagatca ccgataccgg agggaagcag
     4021 cgcgctgccg gcggcgacgc tgatcacctg gttgctcccc atgcgggaga cgggtggcca
     4081 gcgtcgtctg gaacgtgcgg cttgcgcatg tcacctgctg cagatgccgc tgctggcgac
     4141 gccggtcatc gacgtgaccg tgtcgtgata cgctggtcgt gttgcatctc cagccggcag
     4201 ggagggccca cgtggatcgt gtgtcgccag gttcccactc tggcctcgtc gggcgcgacc
     4261 gcgaacttgc cattctgaac gggtgtctga cggccgcgca gggtggccgg ggcagtctcg
     4321 tgttgattag tggcgaggct ggcatcggca agacggccct caccaacagc ttcacgcatg
     4381 ccgcgaccaa tgcgcgactg accgtcctca ccggccactg ctatgaccgt accgagacgc
     4441 ccccctatgg tccctggcgc gaaattggtc agcgcgtcga tgccatcccc gttcctcgta
     4501 tcgacgacgc gacgagccag gcggacctct tcacgcaggc acgcgacttc ctcgctgcga
     4561 tgacggcaga acgaccactc gtgctcgtgc ttgaagatct ccactgggcg gacaacgcca
     4621 gcctcgacct gctacggttc cttgcgcgcg ggtttggtga cctgcctctc ctgtgtgtcg
     4681 cgacctaccg cgatgacgaa gtcgaacggc atcacccgct ggccgggctc gtcccgctgc
     4741 tcgtacgcga ggcgccaacc gagcggctta gcctgcgccc gctcaatacc tcggcagctc
     4801 aggcgctggt gcgggcgcgg tacgactttg cagaaccggc gagcgagcgc ctggccatgt
     4861 atctgatcga gcgcaccgag ggcaacgcgc tgttcatgac cgagctattg cgtagcctcg
     4921 aggaggatcg gctgctcgag cgcgtcgtca gcgatccaca cgcatggagt ctcgcgccga
     4981 caccggtacc gtcgctcctg cagcagatcg tcgacgatcg gctc
//
LOCUS       DASUGJ010000602         6683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1209768, whole genome shotgun sequence.
ACCESSION   DASUGJ010000602 DASUGJ010000000
VERSION     DASUGJ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209768"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFV93_13670"
     CDS             <1..515
                     /locus_tag="VFV93_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166445.1"
                     /translation="RPLARLGWAWLLAGEQLATTAWRVWLPARRGRVIVTDRAAWDTA
                     VEMDVSLPAGARWSRLAIAAMLRLTPRADLSFVLDLSHETARARKPDELWHLGWEDER
                     RRYEALAERYGLRLLSTEGPFDRSSDPLIHDVLMAIMARYETRLNGLLYSNPSQKNVP
                     DPIWAKGSER"
     gene            512..2701
                     /locus_tag="VFV93_13675"
     CDS             512..2701
                     /locus_tag="VFV93_13675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5166446.1"
                     /translation="MKVLVVTNMYPSPALPSFGTFVHDQVEALRAEGVEVDVLSFNGR
                     ESKWKYLSAFPRYWRALRGGGYDLVHAHYILAGVVARAQWGHKVVLTHHGPEVLGQPA
                     WQGPLCKLMTPLFDEVIHVTEQVRRHLRDRDGWVIPCGVDLDAFVPLDRDEARARLGL
                     PAGKPLVLFAGDYWRPEKRFDLVEAAMALVKREMPDAELVLLTKQPHDVVPVYMSACD
                     VLVLTSALEGSPMVIKEAMACNLPIVSVVVGDAAEVIGDTPGCAVVARDPAAIATGLI
                     AALHERRRTDGRTRINHLQHDNIARRILAVYERATQPRRIATSRELPRRGWRRSAPSV
                     CIVRHNYYPDSHVRRDAEALVKAGYDVSVIALRGKGQRARETLNGVDVHRLPVERQRG
                     SALRYAWEYSSFALLAFLRLTRLHLRKRFRVVEIDNMPDILVFSALVPWLTGTPVILY
                     IFDNMPELFAYLRQTSERHPLVRLLAFLERISAAFASRVIVTQEMPRRIAVARGVPEE
                     KLSVVLNCADETIFNRNGITDAPTQNGRFEIVSHGVLLERYGIQVLIEALPAVLTRVS
                     DAHLQIFGAGEYRDTLERRVARLGLEAHVHFRGFAPLDELLTELARADAGYVGMLNDL
                     SLPNKLMEYVVMGVPVIISRWPTFTYYFPEDSVTYFDAGDAGDLACALLRIHAGREAA
                     RERAARARSRYQHYRWEVQREIYLNVYAELAGIKDERAASLAREAAR"
     gene            2698..3900
                     /locus_tag="VFV93_13680"
     CDS             2698..3900
                     /locus_tag="VFV93_13680"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5166447.1"
                     /translation="MKAQLPLRPNEELLLQTVRAAPDPVRLGALLARTDFDWRPFWTL
                     AGDQHVQPLVARVLADSRLACMLPDEARETMTAVRRQTTLHNMSTDAELNRIGAILAA
                     AAIPFAPLKGTQLTRRLFGGLDARRCGDIDILVAESHWQAAHTLLTGAGYQPAAEVRP
                     GVESHAFHGVPLVRVGNGQAFIVELHRKLTDPRFVTIDYRELWQRLEPAESGPPGQME
                     LPAADLLVFLAIHAPKHSTGILRLLADIDHLIAREADRLDWQSVIRLARDWHADTILA
                     CVLQLATALLETPVPDDAIQALRPARWKRVTIPFLVGPETILHPPAAAHLRAKRFRIA
                     YCLMLQRSGRELRSYWHYIMMPPSLRPEHALARVSLAIRQPLDGLAWTALTLASATRD
                     RVRSRAPT"
     gene            4271..>6683
                     /locus_tag="VFV93_13685"
     CDS             4271..>6683
                     /locus_tag="VFV93_13685"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEX5166448.1"
                     /translation="MPESYEADEPEDDDRDTPIQPAPQALRPLQPFALPAQPTPLIGR
                     DQLVNRLANHLESADVRLLTLLGPGGVGKTRLACELARRRERDHELVRFIDLAQFQTS
                     AQVAAVLQSSLGPDRLSGAPDAEQLVLLGNRRTLLVLDNFEHLVVAAPLVADLLAEAP
                     WLQIIATSRTALRLRWEHVCAVPPLMTPPPADRDDAGDLATIPSVALFVTFARRFDPE
                     FSVTRKNARIISDICRQLEGLPLALELAAADLRVMSLERLKERVGRPLDTLIHGQRDM
                     PQRQQTLRATIEWSYSLLSAEEQRISRQLAIFAGGGTIPAIAEVLGLPESSAYDLVAN
                     LADQNLFTIVELNAAGPRVQIQHMVREYLLERLSTDEELPALRDRYMTCYSRLATELA
                     ERLYGPEADETFNRLESDADNFRAILDATGDQQHAGRLRLALALMPFWESKGHLPEGR
                     RRIERLIERLPVDELELRFEAHLAAVHLAILAGDLATARRSLDMASLACTTPGGPAGW
                     ATVTSWAAYIRLLQGDPDGAARLLDHTPELAGADRYRSGAFPTGELRARIAAQRGDDT
                     APDLLAGALNACNRASDHIRSVDLLCLQAEMALGGGDAGSAARLYAEALVRSQVARRP
                     RLRSRPLIGLGDIAIDQLTADEALRHYHEAFLVARDHQDEPSMMRVLERVAILGISSR
                     IYPNLLRMYGLASAIRARLGVARSAYDERLVMPKLDHARSLLGNEEFERIMAGGTTLT
                     IPQVINEMKTGASLRRAGITVTQPAGKSAPLTIKQLTRREREVVQQISKGFTNRQIAA
                     NLGMAE"
BASE COUNT         1108 a         2312 c         2168 g         1095 t
ORIGIN      
        1 aacggccgct ggcccgcttg ggctgggcct ggctgctggc gggcgagcag ttggcgacga
       61 ccgcctggcg cgtttggctg ccggcccggc gagggcgggt gatcgtcacc gaccgagccg
      121 cctgggacac cgccgtcgag atggatgtct cgctgccggc cggcgcacgc tggagccggc
      181 tcgcgatcgc ggcgatgctg cggctgaccc cacgcgccga tctctcgttc gtcctcgacc
      241 tctcgcatga gacggcgcgc gcgcgcaagc ccgacgaact ctggcatctc ggctgggaag
      301 acgagcggcg gcgctatgag gcgctggccg agcgctacgg cctgcggctg ctctcgaccg
      361 aggggccgtt cgaccggtcc agcgacccgc tcattcacga cgtgctgatg gccatcatgg
      421 cccgctacga aacgcggctg aacgggctgc tctactcgaa tcccagccag aagaacgttc
      481 ccgatccgat ctgggcgaag gggagcgagc gatgaaggtg ctggtcgtca ccaacatgta
      541 cccgtcgccg gcgctgccct cgttcgggac gttcgtccac gatcaggtcg aggcgctgcg
      601 ggccgaaggc gtcgaggtcg atgtcctcag cttcaacggc cgtgagagca agtggaagta
      661 cctgtcggcc tttccgcgct actggcgcgc gctgcggggc ggcggctacg acctcgtcca
      721 cgcccactac atcctggccg gcgtcgtcgc ccgcgcccag tggggccaca aggttgtcct
      781 gacccaccac gggccggagg tgcttggcca gccggcctgg cagggaccac tctgcaagct
      841 gatgacgcca ctcttcgacg aggtgatcca cgtcaccgag caggtacgtc gccacctgcg
      901 cgaccgcgac ggctgggtca tcccctgcgg cgtcgacctg gacgccttcg tcccgctcga
      961 ccgcgacgag gctcgcgccc ggctcggcct gccggccggc aagccactgg tgctcttcgc
     1021 tggtgattac tggcggccgg aaaagcgctt tgatctggtt gaggcagcga tggcattggt
     1081 gaagcgcgag atgcccgacg ccgagctggt cctgctcacg aagcaaccgc acgatgtcgt
     1141 gccggtctac atgagcgcct gcgatgtgct ggtcctgacc tcggcgctgg agggctcgcc
     1201 gatggtcatc aaggaggcga tggcctgcaa cctgccgatc gtctcggtcg tcgtcggaga
     1261 tgccgccgag gtgatcggcg acacgcccgg ctgcgccgtc gtcgcgcgcg acccggccgc
     1321 gatcgctacc ggtctcatcg ccgccctcca cgaacgacgc cggaccgacg gccggacgcg
     1381 catcaaccac ctgcagcatg acaacatcgc acgccggatt ctggcggtct acgagcgcgc
     1441 cacgcagccc agacgaattg caaccagcag ggaattgccc cggcgcgggt ggcgccgatc
     1501 ggcgccgtcc gtctgcatcg tccgccacaa ctactatcca gacagccacg tccggcggga
     1561 cgccgaagcc ctggtgaaag ccggatacga tgtcagcgtc atcgccttgc gggggaaagg
     1621 gcagcgggcg cgcgaaacgc tgaatggtgt cgacgttcac cggctgccgg tcgagcgcca
     1681 gcgcggctcc gccctgcgct acgcctggga gtattcgtcg ttcgcgctac tggccttcct
     1741 gcggctgact cggctccacc tgcgcaagcg cttccgggtc gtcgaaatcg acaacatgcc
     1801 ggacatcctc gtcttcagcg cgctcgttcc ctggctgacc ggaacgcccg tcattctcta
     1861 cattttcgac aacatgccgg agctgtttgc ctatctccgg cagaccagcg aacgccaccc
     1921 gttggttcgg ctgctggcct tcctcgaacg gatcagcgcc gccttcgcca gccgggtaat
     1981 cgtcacccag gaaatgcccc ggcgcatcgc agtcgcccgc ggtgttccgg aagagaagct
     2041 gtcggtcgtc ctcaactgtg ccgacgagac gatcttcaat cgcaacggga tcaccgacgc
     2101 gcccacccag aacggccgct tcgagattgt tagccacggc gtcctgctcg aacgctacgg
     2161 gattcaggtg ctgatcgagg cattaccggc cgtcctgaca cgtgtctcgg acgcccacct
     2221 ccagatcttc ggcgctggtg agtatcgtga cacgctggag cggcgggtcg cacggctcgg
     2281 tctggaggca catgttcact ttcgcggctt cgctccgctc gacgaactgc tgacagaact
     2341 cgcccgggcc gacgccggct acgtcgggat gctcaatgat ttatcgttgc ccaacaagct
     2401 gatggagtac gtcgtgatgg gcgtgccggt catcatctca cgttggccga cattcacgta
     2461 ctacttcccc gaggactccg tgacctactt cgacgccggt gacgcgggcg acctggcctg
     2521 cgcgctgctg cgcatccacg ccggccgcga ggcggcccgc gaacgggcgg cccgcgcccg
     2581 ctcgcgctac cagcactatc gctgggaggt acagcgcgag atctacctga acgtctacgc
     2641 cgagctggcg gggatcaagg acgagcgggc cgccagtctc gcgcgggagg cggcacgatg
     2701 aaagctcaac tgcccctccg cccgaacgaa gagctgctgc tccagacggt gcgcgccgcg
     2761 ccggatccgg tccggctcgg cgcgctgctg gcgcgaaccg atttcgactg gcgcccgttc
     2821 tggacgctgg ccggcgacca gcatgtccag ccgctggtcg cccgtgtcct cgccgattcc
     2881 cggctggcct gcatgctccc cgacgaggcg cgtgagacga tgacggcagt ccgcaggcag
     2941 acgacgctgc acaacatgtc gaccgacgct gaactaaacc ggatcggcgc aatcctggcg
     3001 gcagcggcca ttccattcgc gccgctcaag ggaacgcagc tcaccaggcg gctattcggc
     3061 gggctggatg cccgccgctg cggcgacatc gacatcctgg tcgccgagag tcactggcag
     3121 gctgcccata cgctcctcac cggcgccggg taccagccgg ccgccgaggt ccgtcccggc
     3181 gtcgagtcgc acgcgttcca cggcgtgccg ctcgtccgtg tcgggaacgg tcaggcgttc
     3241 atcgtcgagc tccaccggaa gctgaccgac ccgcgtttcg tcacgatcga ctatcgggaa
     3301 ctctggcagc ggctggaacc ggccgagtcc ggcccgcccg gccagatgga gttgccggcc
     3361 gctgacctgc tggtctttct ggcgatccac gcacccaagc acagcaccgg catcctgcgg
     3421 ctactggccg atatcgacca cctgatcgct cgcgaagccg atcggcttga ctggcagtct
     3481 gtcatcaggc tggcgcggga ctggcacgcc gacacgatcc tcgcctgcgt gctgcaactg
     3541 gcgacagcac tgctggaaac gcccgtcccg gacgatgcca tccaggcgtt gcgtccagcg
     3601 cgctggaaac gcgtcacgat cccgtttctg gtcgggccgg agacgatcct gcacccaccg
     3661 gcagccgccc atctacgggc caagcgcttc cggatcgcct attgcctgat gctccagcgc
     3721 agtggccggg agctgcgctc gtactggcac tacatcatga tgccgccgag cctgaggccc
     3781 gaacatgcgc tggcacgcgt ctcgctggct atccgccagc cgctcgatgg cctggcgtgg
     3841 acggctctga cgctcgcctc ggccactcgc gaccgagtcc gctcgcgtgc tcctacctga
     3901 atcatcaggt ggcggaccgc gtgcgtcttt ttacgcaatc agctaagttc gcgcgattgg
     3961 agcgaagtga gcgtacccat tgcgcaagta caggaacggg catatactca cgaagtagat
     4021 cgggagagac agacgaaatc acagttagga aatcgctgcc gtaggggcca gtccgtgagt
     4081 gacgtgctta tcacgaccct gggccgcatc agcgcctggg agagccgcgc tcgatcgcat
     4141 ctccgggtcc gcacgacata ccagggacca ccgatgagtc caataccgcg ggccccgaga
     4201 aggacggctg gtaatagtgc cttcaatgca acaattcgac ttgccgggcc atgggcccaa
     4261 caatgcgtac ttgcccgaat cgtacgaggc agacgagcct gaggacgacg atcgagacac
     4321 ccccattcag ccggcgccgc aggctcttcg gccgctgcaa cccttcgcgc tgccggccca
     4381 gccaaccccg ctcatcggcc gcgaccagct cgtcaatcga ttggcgaacc atctggaaag
     4441 cgccgatgtc cggctgctga cgctgctcgg cccgggtggg gttggcaaga cacgtctggc
     4501 ctgcgagctc gcgcggcgac gcgaacgaga tcacgagctc gtccgtttta tcgacctggc
     4561 gcagttccag accagcgcgc aggtggccgc cgtgcttcag tcatcgcttg ggccggaccg
     4621 cctcagcggc gcacccgacg ccgagcaact cgtcctgctc ggcaaccggc gcaccctgct
     4681 ggtgctggac aacttcgagc acctggtcgt cgccgcgccg ctggtggccg atctgcttgc
     4741 cgaagcgccc tggctccaga tcatcgcgac cagccgaacg gcgctgcgac tgcgctggga
     4801 gcacgtctgt gccgtcccgc cgttgatgac accgccgcca gccgatcggg acgatgccgg
     4861 cgacctggca acgattccgt cagtcgccct gtttgtcact ttcgcgcgcc ggttcgatcc
     4921 cgaattcagc gtcacgcgca agaacgcgcg gattatctcg gatatctgcc ggcagctgga
     4981 gggtcttccg ctggcgttgg agctggcggc tgccgatctg cgggtgatgt cgctggagcg
     5041 cctcaaggag cgcgttgggc gaccgctcga cacgctcatc cacggccagc gcgacatgcc
     5101 gcaacgccag cagacgttgc gggccacgat cgagtggagc tactcgctgc tgtcggccga
     5161 agagcagcgc atcagccggc aactcgccat cttcgccggt ggcggcacga ttccggctat
     5221 cgcggaggtg ctcgggctgc ccgagagcag cgcctacgat ctggttgcca atcttgccga
     5281 ccagaacctg ttcacgatcg tcgagttgaa tgccgccggg ccacgtgtcc agatccagca
     5341 catggtgcgg gagtacctgc tcgaacgcct gtcaaccgat gaggagcttc cggcgcttcg
     5401 cgatcgctac atgacgtgct acagcaggct cgcaacggaa cttgccgagc gcctctacgg
     5461 cccggaggcg gacgaaacgt tcaacaggct ggagtccgac gccgacaact tccgcgcgat
     5521 cctggatgcc accggcgatc agcagcacgc cggccggctg cggctggcgc tggcgctcat
     5581 gcccttctgg gagtcaaagg gccacctgcc ggagggtcgc cggcggatcg agcgactgat
     5641 cgaacggctg ccggtcgacg aactggagct gcgcttcgaa gcccatctgg ccgccgttca
     5701 tctggcgatc ctggcgggtg atctcgccac ggcccggcgc agcctcgaca tggcgtcgct
     5761 agcctgcacg acgccgggcg gtccagccgg ctgggcaacg gtgaccagct gggcggccta
     5821 catccggctg ctccagggcg atccagacgg cgcggccagg ctgctggacc atactccgga
     5881 actggcgggg gcggatcggt atcgatccgg cgcgttcccc accggagagt tgcgggcacg
     5941 gatcgccgcc cagcgcggag acgacaccgc ccctgacctg ctggccggcg cgctgaacgc
     6001 gtgtaaccgg gccagcgatc acatccggtc ggtcgacctc ctctgcctgc aagcggagat
     6061 ggcactgggc ggcggcgatg ccggcagcgc ggcgcggctg tacgccgaag cgctcgtccg
     6121 ctcccaggtc gcgcgccgcc cgcgtctgcg tagtcgccca ctgatcggcc tcggcgacat
     6181 cgccatcgat caactgacgg ccgacgaagc cctgcggcat taccatgaag cgttccttgt
     6241 cgcgcgggac catcaggacg aaccgtcgat gatgcgcgtg ctggagcggg ttgccatcct
     6301 gggcatctcg tcgaggatct acccgaacct gctgcggatg tatggactgg ccagcgcgat
     6361 ccgggccagg ttgggagttg cccgttctgc ctacgacgag cgtttggtga tgccaaagct
     6421 cgatcacgcg cgatcgttgc tgggcaacga ggagttcgaa cgcatcatgg ctggcggcac
     6481 gacactgacg attccacagg tcatcaacga gatgaaaacg ggcgccagcc tgcggcgggc
     6541 cggcatcact gtcacgcaac cggccggcaa atcagcgccg ctgacgatca agcagctgac
     6601 ccgccgcgaa cgcgaggtcg tccagcagat cagcaagggc tttaccaacc ggcagatcgc
     6661 cgccaacctc ggcatggccg aac
//
LOCUS       DASUGJ010000603         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1765698, whole genome shotgun sequence.
ACCESSION   DASUGJ010000603 DASUGJ010000000
VERSION     DASUGJ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765698"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..899
                     /locus_tag="VFV93_13690"
     CDS             105..899
                     /locus_tag="VFV93_13690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5166449.1"
                     /translation="MSLRREAELQYLTTDPLKVRIETHQRYSERLIDLEAESAVAFAL
                     ISGESILDVGCGTGAFLSYLRRNGSQGRLVGLDRSDAMIAEAAREPSIEWTVGDVERL
                     PFTDGEFDRVSARHMLYYIDDLQSALCELARVTKPNGLFLATTNAARSTPLIDDLYLD
                     LLAAFGLPRRQHAAGEFHTETALAALSTVWPHVEDIILDNACIFTSSEPIVRYVATLL
                     PSVDGADSDMRVEMLNWLEREAASRLAVLGGIWRDPKIVGIYRCRR"
     gene            1092..3305
                     /gene="argS"
                     /locus_tag="VFV93_13695"
     CDS             1092..3305
                     /gene="argS"
                     /locus_tag="VFV93_13695"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5166450.1"
                     /translation="MFRTYEQQAAAAINDIITDLGFRRREFDMRAIPFSGSWGTSSSI
                     AFALANEATVEEPAAEDESLSKKERKALQQNRVREKATELAEQIAAALQARPDYARVE
                     AVNGYINIYFDTNQVANEVIGTVRREGADFGRGVAQSDKVMIEYSQPNTHKEFHVGHL
                     RNTCLGEALARITAFAGFETLRATYPGDIGLHVIRTLWCFKTFHAGEAEQIPADQRGR
                     WLGGLYTEAVRRLEYRDEVVGLLNELAKMDAGFSAMIDRMAKDLYKAGAPGEDVAYLL
                     GQIANQREIKTDALYDDTTVARFWPIVGRQLEDELDLIRREGPQQAPPVPSHSGGPPV
                     IPPLVTLEDTEDRYARWQRLGEHLDWWPHVPEWQQGVRDTFQLWEREDPEFMSLWWDT
                     RQWSLDGFERIWAELGVHFDVYFFESQVEAEGREIVRDLLERGIAEVSDGLPVVKIDE
                     KLGLEKETYRTMPILRSDGTTLYATKDLALTKRKFEDYGIDSSVWVIDVRQSLYMQQI
                     FKIMELLGFEQAEKCYHLGYEIVTLPEGAMSSRKGNVILYDDLAREARARARDIIEEK
                     AKQAHLSDAAKDTIAEQVGIGSIIYAMLARDNNRVIVFDMDEALSFDGHAAPYIQYAH
                     ARACRILERVEEMPTGELVFEDLQPEEIDLIQQIGNFPTEVQRAAAEHKPLLISTWVY
                     DLARRFNDFYADIEKRPVLTAPEPTRAMRIALVDATRQALANGLALLAIAAPEAM"
     gene            3308..3742
                     /locus_tag="VFV93_13700"
     CDS             3308..3742
                     /locus_tag="VFV93_13700"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5166451.1"
                     /translation="MVLVFVNVESAVYHDGRYLMIVRGEEEDHAAGMLAFPGGGLDYV
                     EMPDILEQTAVREIFEETGVSVDDVQFVEAHSFTAPDGTPVVDVVFLSRYDAGTPLIT
                     DPGEVAALRWMTAAEILADHACPPWMSPSLALVERKRQALGW"
     gene            complement(3886..>4176)
                     /locus_tag="VFV93_13705"
     CDS             complement(3886..>4176)
                     /locus_tag="VFV93_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166452.1"
                     /translation="GGSAIFSPSVATRILTLFSDPRPAPVAVFPMLTDREREILERIA
                     QGYPNPRIAQQLGISVKTVSNHVSVIFGKLQVADRAEAIDRAREAGLGQRQP"
BASE COUNT          779 a         1307 c         1378 g          712 t
ORIGIN      
        1 agcgaaggac ctctccgcgt cagccgatca cgcggtcaaa gcggagaggt ccttcgctgc
       61 ggctcaggac gacagaggta gtctttccag cctggggaga tgcgatgagc ttgcgccgcg
      121 aagccgaact gcaatacctg acgaccgatc cgctcaaggt gcggatcgag acacaccagc
      181 gctactcgga gcgcctcatc gatctggaag ctgaaagcgc cgtcgcgttt gcactcatta
      241 gcggcgaatc gatcctagat gtcggttgcg ggacgggagc gtttctgtcc tatctgcgtc
      301 gcaatggcag tcaggggcgg ttggtcggac tcgatcgctc ggatgcgatg atcgctgagg
      361 cggcgcgcga accgtcgatt gaatggaccg tcggtgatgt cgagcggctg ccgttcacgg
      421 acggtgagtt cgaccgcgtc tcggcgcgcc atatgctcta ctacatcgat gatctgcaat
      481 ccgcgttgtg cgaactggcg cgagtcacga agcccaacgg gctgttcctc gcgacaacga
      541 acgccgcgcg ctcgacgccg ttgatcgacg acctgtacct cgacctgctg gcggcattcg
      601 gacttccacg gaggcagcac gcagctggcg agttccatac cgaaactgcg ctcgcggcgc
      661 tcagcaccgt ctggccacac gtcgaagaca tcatcctcga caacgcgtgt atctttacct
      721 cgtctgaacc gatcgtccgc tacgtcgcaa ccctgctgcc ctcggttgat ggcgcggact
      781 cggacatgcg agtcgagatg ctcaactggc tcgaacgcga ggccgcatcg cgcctggctg
      841 tactcggcgg aatctggcgc gacccgaaga tcgtcgggat ctatcgctgt cgcaggtaat
      901 tgcgcgccaa agaagcaggg gccgtgcctt gtgccggccc tgcgttgcct cccgaacgcg
      961 tcgagtgggg aacagggccg gcacaaggca cggcccctac ttctttggcg ctggcgaact
     1021 gaccgcctgt ggaatacttc gcgatgcgcg tatttgggag cgttatcagg cgagacgacc
     1081 ggagagagag tatgttccgc acctacgaac agcaggcggc agccgccatc aacgacatca
     1141 tcaccgatct gggcttccgt cgtcgcgagt tcgatatgcg tgcgattcca ttctccggct
     1201 cgtggggaac gtcgtcgtcg atcgcgttcg cgctggcgaa cgaggcgacc gtcgaggaac
     1261 cggccgccga ggacgaatcg ctctcgaaga aggagcgcaa ggcgctccag cagaaccggg
     1321 ttcgcgagaa ggcgaccgag cttgccgagc agatcgcggc agcgttgcag gcgcggcccg
     1381 actatgcgcg ggtcgaggcc gtcaacggct acatcaacat ctacttcgac accaatcagg
     1441 tcgcgaatga ggtgatcggc accgtccggc gcgagggggc cgatttcgga cggggcgtgg
     1501 cacagtctga caaggtcatg atcgaatact cgcagccgaa cacgcacaag gagttccacg
     1561 tcgggcacct gcgcaacacc tgccttggcg aggcgctggc gcggatcacg gccttcgccg
     1621 gcttcgagac gctccgcgcg acataccctg gcgacatcgg cctgcacgtc atccgcacgc
     1681 tgtggtgctt caagaccttt cacgccggcg aggcggagca gatcccggcc gatcagcgcg
     1741 ggcgctggct cggagggctc tacacagagg ccgtccggcg gctggagtat cgcgacgagg
     1801 tcgtcgggct gctgaacgag ctggcgaaga tggacgccgg cttctcggcg atgatcgacc
     1861 ggatggcgaa ggatctctac aaggcgggtg cgcccggcga ggatgtcgcc tatctgctgg
     1921 gtcagatcgc caaccagcgc gagatcaaga ccgacgcgct ctacgacgac acgaccgtcg
     1981 cgcgcttctg gccgatcgtc ggccggcagc tggaagacga gctggacctg atccgccgtg
     2041 aaggcccgca gcaggcgccg ccggtgccgt ctcactccgg agggccgccg gtcatcccgc
     2101 cgctggtgac gcttgaggac accgaggacc gctacgcccg ctggcagcgg ctgggcgagc
     2161 atctcgactg gtggccgcac gtgccggaat ggcagcaggg cgttcgcgac acattccagc
     2221 tctgggaacg ggaagatccg gagttcatgt cgctctggtg ggacacgcga cagtggagcc
     2281 tggatggctt tgaacggatc tgggccgaac tgggtgtcca cttcgacgtc tatttcttcg
     2341 agagccaggt cgaggccgag ggccgagaga tcgtccgcga tctgctggag cgcggcatcg
     2401 ccgaggtcag cgatggcctg ccggtcgtca agatcgacga gaagctcggg ctggagaagg
     2461 agacctaccg gacgatgccg atcctgcgct cggacggcac gacgctctac gccaccaagg
     2521 acctggcgct gacgaagcgc aagttcgagg actacggtat cgacagctcg gtctgggtca
     2581 tcgacgtgcg ccagtcgctc tacatgcagc agattttcaa gatcatggag ctgctgggct
     2641 tcgagcaggc tgagaagtgc tatcacctcg gctacgagat cgtcacgctg ccggaagggg
     2701 cgatgtcgag ccgcaagggc aatgtcatcc tctacgacga cctggcgcgc gaggcgaggg
     2761 cacgggcgcg ggacatcatc gaggagaagg ccaagcaggc gcatctgagc gacgctgcga
     2821 aggacacgat cgcggaacag gttggaatcg gcagcatcat ctacgcgatg ctggcgcgcg
     2881 acaacaaccg tgtcatcgtc ttcgacatgg acgaagcgct ctcgttcgac ggccacgccg
     2941 cgccatacat ccagtacgct cacgcccgcg cctgccggat tctggaacgc gtcgaagaga
     3001 tgccgacggg cgagctcgtc ttcgaggatc tccaaccgga ggagatcgac ctgatccagc
     3061 agatcggcaa cttcccgacc gaggtccagc gagcagccgc cgagcacaag ccgctgctga
     3121 tctcgacctg ggtctacgat ctggcgcgcc gcttcaacga cttctacgct gatatcgaga
     3181 agcggccggt gctaacggcg ccggaaccaa cccgcgcgat gcggatcgcc ctcgtcgacg
     3241 ccactcgcca ggcgctggcg aacgggcttg cactgctggc aatcgccgca cccgaggcga
     3301 tgtagtagtg gtactcgtct tcgtcaacgt cgaatcggcc gtctaccacg atggccgcta
     3361 tctgatgatc gtccgaggtg aggaagagga ccatgcggcc gggatgctcg cgttccccgg
     3421 cggcggcctc gactacgtgg aaatgccgga tatcctggaa cagacagccg tcagagaaat
     3481 atttgaggag actggtgtct ccgttgacga tgtccagttc gtcgaagcac actcgttcac
     3541 cgcgcctgat ggcactcccg tcgttgacgt cgtcttcctc agccgctacg acgccggcac
     3601 accactcatt accgatcccg gcgaggtggc cgccctgcgc tggatgaccg ctgccgaaat
     3661 cctggccgac cacgcctgcc cgccgtggat gtcgcccagc ctcgcgctgg tcgaacgcaa
     3721 acggcaggcg ctgggctggt aggctcacca tcccgcaatt caacgatcca tcctcgatcc
     3781 ggtacgggcg atatcgcaat atcgcccttc ttcgcgcgcc gggcgtctga aagggcgata
     3841 ttgcgatatc gcccgtaccg gattgaggca ttgattggga ccggatcagg gctgccgctg
     3901 gccaagcccg gcttcgcggg cgcggtcgat cgcttcggcg cggtcggcga cctggagctt
     3961 gccgaagatg accgagacgt ggttgctgac cgtcttgacg ctgataccga gctgctgggc
     4021 gatgcgcggg ttcgggtagc cctgcgcgat ccgctccagg atctcgcgct cgcggtcggt
     4081 cagcatcggg aagaccgcca ccggggccgg gcgtgggtcg gagaagagcg tcaggatgcg
     4141 ggtcgcgacc gatgggctga agatggccga cccacc
//
LOCUS       DASUGJ010000604         8773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_653323, whole genome shotgun sequence.
ACCESSION   DASUGJ010000604 DASUGJ010000000
VERSION     DASUGJ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653323"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /gene="nadA"
                     /locus_tag="VFV93_13710"
     CDS             complement(<1..587)
                     /gene="nadA"
                     /locus_tag="VFV93_13710"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX5166453.1"
                     /translation="MAVSLVVPGARTVPMVVEEQASCVPEEGTGAYDLLRRYGVNSPR
                     QTKIPREYFTMPQDEREARTWDYRRQLGERLVILGHHYQREEVVQFADFRGDSYKLSK
                     QAAERADADFILFCGVHFMAESADILCRPHQQVILPNLEAGCSMADMAKIDDVADAWD
                     ALAELGISGVVPITYMNSAASLKSFTGEHGGIVCTS"
     gene            1055..2341
                     /locus_tag="VFV93_13715"
     CDS             1055..2341
                     /locus_tag="VFV93_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166454.1"
                     /translation="MANLSRASRREARTNAPAVEAIVASPPEATGMLHSLEMYRPFRL
                     LFSGTLATNSAFWMYQIAVGWLALQTTDSPFFVGLTGFAGGIPLLFLALPAGVVIDRV
                     DRRTVLMAAQVGVMTVAGLFALLVGTGLIRPWSILLLAFLYGVVMSFVFPTRNAIVPD
                     LVDRTHLANAVALNAAGQNATRVVGPSLAGLLIALIGISGTFAIAAAMQILALYWTSQ
                     LPANGPAATSGGSTLWTSLMVGLRVVAKDPFLVGLILLASATTVLIMPYINLMPVFAR
                     DVMGLGSAGLGVLMASSGLGSVTGALAVARYRRLATWPGIQLITAGGFAGLVLLFAVT
                     PSVLMATVLLFVAGLMSAAYMAINQTVLQLKCDDDVRGRVLSIYLLTWGMLPLGQLPL
                     GALADRIGASAATAIACTIALGVITLLALRFPSLRS"
     gene            2762..4726
                     /locus_tag="VFV93_13720"
     CDS             2762..4726
                     /locus_tag="VFV93_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5166455.1"
                     /translation="MTLHESSTLDLLREQILAGQLSRRSVLKRAMVLGLSAPVIAGLL
                     AACGGDDDDDDDGGTTPAATNTVASGGQPTTAASEPTATTGGSSGTTASPTSPSTSAE
                     PTATTASAPPVEAGGGGLIKLLWWQAPTILNSHLAQGTKDYDASGLILESLANVAADG
                     SLVPRLAAEIPSLENGAVAADGLSVTWTLREGVKWHDGEPFTAKDVAFTYTYLIDEKT
                     TSTTQGAYQAVSSVEAIDDLTVRVNFSEPNPGWFGPFVGPLGMIVPEHIMKDFVGDKA
                     RDAPFNLAPTGTGPYKLNEFRPGDVVVYDRNEDYWDPGKPFFDRVEMKGGGDAASAAR
                     AALVSGEVDFAWNLQVEAEILEQMADEGDGLLVPIPSPNVERILVNFADPNQEVNGAR
                     SEPSTQHPFLQFKEVRQALTYAIDRDTVATQLYGPTGEATTNILVAPDAFASPNTSAE
                     FNTDKAAEMLEAAGWTGSPRSKDGVEMKVIYQTSANTVRQKTQEIVKAAFEEIGIPME
                     IKAIDAAVYFSSDAGNPDTASHFYADLEMYTNGPSSPYPISYMTGFISINPDVDIAQQ
                     SNDWAGTNEMRWVNADYNALYQQAQVELDPAKQADLFIGMNDLIVNEVAQIPLVHRAS
                     VVAHRADLVGVEGSGWDSNAWNIPNWKLEG"
     gene            5226..7187
                     /locus_tag="VFV93_13725"
     CDS             5226..7187
                     /locus_tag="VFV93_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5166456.1"
                     /translation="MEHESSRLELLREEILSGKLNRRTVLKRAMLMGLSAPVIAGLLA
                     ACGSDDDDDDDTPAATNTTATGSGQATTPAGEPTATAAESGEGTEEASPTTGTSTGTG
                     STDTPGKGRGVADLVKILYWQAPTILNTHFSQGTKDSAAASLVLEPLIDIAADGTLIP
                     VLAAEIPSLENGGVAEDGLSVTYKLKEGVMWSDGEPFTANDVKFTFDYASDEAATTTS
                     IAAFDPVESVEVVDDLTVRINFKEPNPGWYGVFSTAFNGGTVLPMHALQDAMGEKARD
                     APFNLNPVGTGPYKVVEFRPGDVVSYEINENYREPDKPYFKKVELKGGGDATAAARAA
                     LESGETDWGWNLQVENSILEPMAADSDVGVLVITPSVNVERILVNQADPNEEVNGARS
                     EPSTKHPFLQFLEVRQAFALGCDRDTVATELYGPAGKATSNLLVAPANFASPNTSYTY
                     DPEKGAQMLEDAGYTGSPRAKDGVELSVIYQTTINPLRQKTQEIIKQGWEQMGIAVEL
                     KSVDASVFFASDAGNPDTASHFYTDFEMFTNGPASPYPISYMASWKSVDPAVDIAQQS
                     NAWAGPNAVRWVNADFNDLYTQALTELDPDTQVELFVGMNDLAVNDIAEIPLVHRADV
                     VAAAKKLKGYNPSPWTTDLRDIANWYFEE"
     gene            complement(7268..8203)
                     /locus_tag="VFV93_13730"
     CDS             complement(7268..8203)
                     /locus_tag="VFV93_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5166457.1"
                     /translation="MRVGLGLLNAGETSRYSSLAHVAQHAEALGYDSLWVTERDPFPL
                     ASHSRAGAFTGELHLSRKPIDSTLDALAIAAINTDRIRLGVSLPNIPFYSPTEVGESL
                     AALDVLSDGRVQVGLGLGWTPADFVRVSLALADRQTPASEFVRAMRAVWDGLSVGFTS
                     EYYSMPAARGVITPVQRPSLPVMLTAFAPAAVQPPAMLLRGGSPLMAAMSSEEMAESL
                     RVVTGRSRELGLVVRAVVRLTDGPIGESRAMFTGSAGQLRDDFALIEALGADEVQFDL
                     SRMPFELDNSRLFDVVAQLREMVTQVADVPAAVAA"
     regulatory      8482..8626
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         1612 a         2927 c         2774 g         1460 t
ORIGIN      
        1 gacgtgcaga cgatgccgcc atgctcgccg gtgaacgact tgaggctggc agccgagttc
       61 atgtaggtga tcggaacgac gccgctgatg ccgagctccg ccagcgcgtc ccaggcgtcg
      121 gcgacatcgt cgatcttggc catgtcggcc atcgagcagc ccgcttcgag attgggcagg
      181 atcacctgct gatggggccg gcagaggata tcggcgctct cggccatgaa gtggaccccg
      241 cagaacagga tgaagtcggc atcggcccgc tcggcggcct gcttcgagag cttgtacgaa
      301 tcgccacgga agtcggcgaa ttggacaacc tcttcgcgct ggtagtgatg accgagaatg
      361 accagtcgct caccgagctg gcggcggtag tcccaggtcc gcgcctcgcg ctcgtcctgc
      421 ggcatcgtga agtactcgcg cggaatcttc gtctgtcgcg gcgaattgac accgtagcga
      481 cgcaacagat cgtaggcgcc ggtgccttcc tccggcacgc agctcgcctg ctcctcaacc
      541 accatcggaa ccgttcgcgc acccggcacg accaatgaga cagccatcgc tccctggaca
      601 ctttcattcc gctgggccaa cccgccgcac cgttgcggca ccctcccagg ccggattagt
      661 gtagcactga cacttattag gtgtcaatat gacacttgct tgggatcgtg acgcgtgacg
      721 cgtcacacat gctgcgttcc agacgatcgg cgttggcgaa gaccgtcatc cctcacccct
      781 gtcatcctga gccgcagcga aggatcgcct ggcgctcacg cagcacgttg cgacccagga
      841 tgcacacgct agtctgccga accacgcgct catggccctt acgttcagcg atccttcgct
      901 gcggctcagg atgacagggg tggcgggtgc ggtcctcgtg gacgccgtgc ggctgctgcg
      961 cgtcacgtat cacgcgtcac gcgtcacgcg tcacgccgtc ccgtgctacc ctgtcgccgg
     1021 ctgagtgaga ccctgagcgg aggttgagag gctgatggca aatctgagtc gtgcgtcgcg
     1081 tcgtgaggcg cgtacgaacg cgcctgccgt cgaagcgatc gtcgcctcgc cgcccgaggc
     1141 gaccgggatg ctgcactcgc tggagatgta ccgtcccttt cgcttgctct tctcgggaac
     1201 gctggcgacc aactccgcct tctggatgta tcagatcgcc gtcggctggc tggcgttgca
     1261 gacgaccgac tcgccgttct tcgtcgggct gacaggcttt gccggcggga ttccactcct
     1321 ctttctggcg ctgccggccg gtgtcgtgat cgaccgggtc gaccggcgga cggttctgat
     1381 ggcggcccag gtcggcgtga tgacggtggc ggggctcttc gcgctgctgg tcggaacggg
     1441 cctgatccgg ccttggtcga tcctgttgct ggcgtttctg tacggtgtcg tcatgtcgtt
     1501 cgtcttcccg acccgcaacg cgatcgtgcc ggacctggtg gaccgcacac acctcgccaa
     1561 cgccgtcgca ctcaacgccg ccggccagaa cgcgacccgc gtcgtcgggc cgtcgctggc
     1621 cgggctgctg atcgcgctga tcggcatctc cggaaccttc gcgatcgcgg cggcaatgca
     1681 gattctggcg ctctactgga catcgcaact gccggcgaac ggaccggcgg caacctcggg
     1741 cggctcgaca ctctggacga gcctgatggt cgggttgcgc gtcgttgcga aggatccgtt
     1801 tctggtcggc ctgatcctgc tagcgtcggc gacgacggtg ctgatcatgc cctacatcaa
     1861 cctgatgccg gtcttcgctc gtgatgtgat ggggctcggt tcggccggcc tcggtgtcct
     1921 catggccagc agtgggctgg gctcggtcac cggcgcgctg gcggtcgccc gttaccggcg
     1981 cctggcaacc tggccgggaa tccagctcat tacggccggc ggatttgcgg gactggtgct
     2041 gctgttcgcg gtcacgccgt cagtcctgat ggcgacggtg ctgctgttcg tggccgggtt
     2101 gatgagcgcc gcctacatgg cgatcaacca gacagtcctc cagctcaagt gcgatgatga
     2161 cgtgcgcggg cgggtcctct ccatctacct gctgacctgg gggatgctgc cgctcggcca
     2221 gctcccgctc ggcgcgctgg ccgaccggat cggcgcgtca gccgcgacgg cgatcgcctg
     2281 cacgatcgcg ctcggcgtca tcacgttgct cgcactccgg ttcccatcgc tgcggtcctg
     2341 acttgtcatc cttcggctca cgcctcagga tgactaagcc gggggttgcg aacgacgcga
     2401 tttctggtat tgtgaagtcg gtttcacgaa caattcgact gtcctgaacg caactaccta
     2461 tctgggtgtt cctcaattat tgtccggctc accgctgacg ctgaggacat gcgcaacggc
     2521 gggttaaacg cttaccggag ccggacacgc accgggcttg catcttcaga cacgatgccg
     2581 gcgcaggtgt gtacccgacg atcgccacgc cggtggcgtc tcgccgggag gtcgacgcat
     2641 gcacgctccc cgcgtgcgac gtgggccagt tgacacggaa gccgcgccgg atcgccaggc
     2701 cagaacggcg cgccggcaat gatggtcaca aagggtgtgg ggatttcaga aaggaacgca
     2761 cttgacactg cacgaatcct cgacgcttga tctcctgcgg gaacagatcc tcgctggtca
     2821 gctcagccgc cggtcagtcc tgaaacgcgc gatggtgctg gggctttccg ctcccgtcat
     2881 cgccggattg ctcgccgcct gtggcggtga cgacgatgac gacgatgacg gcggcacgac
     2941 gccggccgcg accaatacgg ttgcaagcgg cgggcagcca acgacagccg ccagtgagcc
     3001 gacggcaaca acgggcggta gctccggcac aacggcgtcg ccaaccagcc ccagcaccag
     3061 cgccgaacca acggcgacga ccgcatctgc gccgccggtt gaggccggtg gtggcggtct
     3121 catcaagctg ctctggtggc aggcgccgac gatcctcaac tcccacctgg cgcaagggac
     3181 gaaggactac gatgcctccg gcctgatcct cgagtcgctg gccaacgtcg ccgccgatgg
     3241 cagcctcgtc ccacggctgg cggccgagat cccatcgctg gagaatggcg cggtggcggc
     3301 cgacggcctc agcgtgacct ggaccctccg cgaaggcgtc aagtggcacg acggggagcc
     3361 attcaccgcc aaggatgtcg cctttaccta cacctacctg atcgacgaga agacgacatc
     3421 gacgacacag ggcgcctacc aggctgtgtc gtcggtcgag gcgatcgatg atctgaccgt
     3481 tcgggtcaat ttctccgagc cgaaccccgg ctggttcggc ccgttcgtcg gcccgctggg
     3541 catgatcgtc cccgagcaca tcatgaaaga cttcgttggc gacaaggccc gcgacgcacc
     3601 attcaacctc gcaccgaccg gaaccgggcc gtacaagctg aacgagttcc ggccgggcga
     3661 tgtcgtcgtc tacgaccgga acgaggacta ctgggacccg ggcaagccct tcttcgatcg
     3721 ggttgagatg aagggtggag gcgacgcggc ctcggcagct cgcgccgcgc tcgtctccgg
     3781 cgaggtggac ttcgcctgga acctgcaggt cgaagccgag atcctcgagc agatggctga
     3841 cgaaggcgac gggctgctgg tgccgatccc gagcccgaac gtcgagcgta ttctggtcaa
     3901 cttcgccgac ccgaaccagg aagtcaacgg cgcccgctcc gagccaagca cccagcaccc
     3961 cttcctgcag ttcaaagagg tccgccaggc gctcacctac gcgatcgacc gggacacggt
     4021 cgccacccaa ctctacggac cgaccggcga ggcgacaacc aacatcctcg tcgctccaga
     4081 tgcgtttgcg tcgcccaaca cctcggccga gttcaacacc gacaaagcgg ctgagatgct
     4141 ggaagccgcc ggctggactg gctcaccgcg ctccaaggac ggcgtcgaga tgaaggtgat
     4201 ctaccagacc tcggccaaca cggtccgcca gaagacgcag gagatcgtca aggctgcttt
     4261 cgaggaaatc ggcatcccga tggagatcaa ggcgatcgac gcggccgtct acttctcctc
     4321 tgatgccggc aacccggaca cggccagcca cttctacgcc gacctggaga tgtacaccaa
     4381 cgggccatcc agtccgtatc ccatctccta catgaccggc ttcatctcga tcaacccgga
     4441 cgtcgacatc gcccagcaat cgaacgactg ggccggcacg aacgagatgc gctgggtgaa
     4501 cgcagactac aacgcgctct accagcaggc ccaggtcgaa ctcgacccgg ccaagcaggc
     4561 cgatctcttc atcggcatga acgacctgat cgtgaacgag gtggcacaga ttccactggt
     4621 ccaccgcgcc tcggtcgtcg cccaccgtgc cgatctggtg ggtgtcgagg gctccggctg
     4681 ggattccaac gcctggaaca tcccgaactg gaagctcgaa ggctaatcca gcaacacgat
     4741 tcgcaacggc aacaggcccg ggcagccagc ccgggcctgt tgcatacatc cagtttgcca
     4801 ctctcagacg tacgaacagg cgagcgttac cagatcgaca gccgccggcc agtacagggt
     4861 ctccagttgt cgtctggtat tgtctgcacg gttttacctg tcagcgaagg gctgttctct
     4921 ggtcggatct cgacggaagg gacgttcgcg ccaacggccg gcgacgttgt cgaaacagcg
     4981 tcgtacgccg ataccactgg cacgaacgtg ggtttggtct ccgcgtcttt cgggcaccac
     5041 ccatcaccca cagaacaacg gcgaccgcgc cagcgcgtcg ccggatagtt gccgggcgtc
     5101 cggggagatc aagccgcgtg caggcacgcg agagatgaac catgtcacgg acgggttact
     5161 gccaggcgcg ttgtgccagc aggtccggca tggggtgtga ggaaagctga aagggctctg
     5221 actggatgga acatgaaagc tccaggctcg aactcctgcg ggaagagatt ctcagcggca
     5281 agctgaaccg ccgcacggtc ctgaagcgcg cgatgctgat gggtctcagc gcgccggtca
     5341 tcgccggcct gctggccgcc tgcggcagcg atgatgacga tgacgacgat acgccggccg
     5401 ctaccaatac aaccgcaacc ggcagcgggc aggccacgac acccgccggc gaaccaaccg
     5461 ccaccgccgc tgaatcaggc gaaggtaccg aagaggcatc accgacgacc ggcacctcga
     5521 ccggtaccgg cagcactgac acgcccggca agggacgcgg cgtcgccgat ctggtcaaga
     5581 tcctctactg gcaggctcca acgatcctga acacccactt ctcacaaggt accaaggact
     5641 cggccgcggc cagcctcgtg ctcgaaccgc tgatcgacat cgccgctgac gggacgctga
     5701 tcccggtcct ggcggccgag ataccgtcgc tcgaaaatgg tggcgtcgcc gaagacggcc
     5761 tgagcgtcac ctacaagctc aaagagggcg tcatgtggtc ggacggcgag ccgttcacgg
     5821 ccaacgatgt caagttcacc ttcgactacg cctccgatga agcggcgacg acgaccagca
     5881 tcgcggcttt cgatccggtc gagagcgtcg aggtcgtcga tgatctgacc gtccgcatca
     5941 acttcaagga gccgaacccc ggttggtacg gcgtcttctc gaccgccttc aacggcggca
     6001 cggtcctccc gatgcacgcc ctccaggacg cgatgggcga gaaagcgcgc gacgcaccgt
     6061 tcaacctgaa tccggtcggt accggcccct acaaggtcgt tgagttccgg cccggcgacg
     6121 tcgtcagtta cgagatcaac gagaactacc gcgagccgga caagccctac ttcaagaagg
     6181 tcgagctcaa gggtggcggc gacgcgacgg cagccgcccg cgccgcgctc gaatcgggtg
     6241 agaccgactg gggctggaac ctccaggtcg agaattcgat cctggagccg atggccgccg
     6301 atagtgatgt cggcgtgctc gtcatcacgc cgtcggtcaa cgtcgagcgc atcctggtca
     6361 accaggccga ccccaacgaa gaggttaacg gcgcgcgctc cgagccgagc accaagcacc
     6421 ccttcctgca attcctggag gtgcgccagg cgttcgcgct cggctgtgac cgcgacacgg
     6481 tcgcgaccga actgtacggg ccggccggaa aggcgacctc caacctgctc gtcgcgccgg
     6541 caaacttcgc ctcgcccaac accagctaca cctacgaccc ggaaaagggc gcccaaatgc
     6601 tggaggacgc cggctatacc ggctcaccgc gcgccaagga tggtgtcgag ctctcggtca
     6661 tctaccagac gacgatcaac ccgctgcgcc agaagaccca ggagatcatc aagcagggct
     6721 gggagcagat ggggattgcc gtcgagctga agtcggttga tgccagcgtc ttcttcgcat
     6781 cggacgccgg caaccctgac acggccagcc acttctacac cgacttcgag atgttcacca
     6841 acggcccggc cagcccgtat ccgatctcct acatggccag ctggaagtct gtcgatccgg
     6901 ctgtggacat cgctcagcag tccaacgcct gggccgggcc gaacgccgtc cgctgggtga
     6961 atgccgactt caacgacctc tacacccagg cactaaccga gctggacccg gacacccagg
     7021 tcgagctgtt cgtcggcatg aacgatctcg ctgtcaatga catcgcggag atcccgctcg
     7081 tccaccgcgc cgacgttgtc gccgcggcca agaagctgaa gggctacaac ccatcgccgt
     7141 ggacgaccga tctgcgcgat atcgcgaact ggtacttcga ggagtaagag ggattcggga
     7201 ttcggggaca ttgaagagcg acgctccgct caataccccc ggatcccgat tcccgtttcc
     7261 cgatccacta cgcagcaacg gcagcaggca cgtctgcgac ctgcgtaacc atctcgcgga
     7321 gctgggcaac gacgtcgaac aggcggctgt tgtccagctc gaacggcatc cggctcaggt
     7381 cgaactggac ctcgtcggcg ccgagagcct cgatgagggc gaaatcgtcg cgaagctgcc
     7441 cggccgagcc ggtgaacatc gcccgcgact cgccgatcgg gccgtccgtc aggcggacaa
     7501 cggcgcgaac gaccagccca agctcacgcg accggccagt gacgacccgc aacgattcgg
     7561 ccatctcctc actggacatc gccgccatca gcggtgagcc gccgcgcagc agcatcgcag
     7621 gcggctggac ggccgccggc gcgaaggccg tcagcatcac cggcagcgac gggcgctgga
     7681 ccggcgtgat gaccccgcgc gctgccggca tcgagtagta ctcgctggtg aacccaaccg
     7741 agagaccatc ccagaccgcc cgcatggcgc ggacgaactc gctggccggc gtctgccggt
     7801 ccgcgagcgc caggctgacc cggacgaagt cggccggcgt ccagccgagg ccaaggccga
     7861 cctgaacgcg gccgtccgag aggacatcca gcgcggccag gctctcgcca acctcggtcg
     7921 ggctgtagaa tgggatgttc gggaggctga cgccgagccg gatccggtcg gtgttgatcg
     7981 cggcaatcgc gagcgcgtcc agcgtcgagt cgattggctt gcgagacagg tgcagctcgc
     8041 cggtgaaggc accggcgcgg ctgtggctcg ccagcgggaa cgggtcgcgc tccgtcaccc
     8101 agaggctgtc atagccaagc gcctccgcgt gctgggcgac gtgggccagc gacgagtacc
     8161 tgctggtttc tccggcattc agcaatccaa gtccgacgcg catcgtgcag tccttccgct
     8221 gatagcccgg tcggacagat cctgaccggc aacaatggct tcatgtctca acgacggtgg
     8281 cgagcatcag cgcttcgtta ccgtcgccac cagactaggg cggaagtcgc accgtcacac
     8341 cgctcgtggg accagaacaa cagcctgtcc ggctgggacg atcacctgtc catctgggcg
     8401 gatcgaactg gtgtggagta ctgcgtaatt tcgcccattc ccttactcgc ggaatctggg
     8461 ctatactggt cgcgacaagg cgaggtcctt cggggcaggg tgcggggagc gatccccaat
     8521 tcccgatcgg cggtagagcc cgcgagcgag gcggcacgac aaacgtctcg catgatccgg
     8581 tgagactccg gggccgacgg tatagtccgg atgaaagaag gctcgcggta gagacagtcc
     8641 ggttccggcc ggtgcgctgg cggcaatgct gcccgtgctg agccgagtcc cgtacctgac
     8701 gctactgatc gccccgaagg cagctgcctt cgggcttttt gttgtccgaa ggcacctgcg
     8761 agccggccgg tcg
//
LOCUS       DASUGJ010000605         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_238227, whole genome shotgun sequence.
ACCESSION   DASUGJ010000605 DASUGJ010000000
VERSION     DASUGJ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238227"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VFV93_13735"
     CDS             <1..1220
                     /locus_tag="VFV93_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5166458.1"
                     /translation="PDAIKADDPCLLEAMTPLGLVAFSDELRPEARTTIEGFREAGIA
                     LKIISGDNPDTVAALARQAGFPHDLQVVSGLELEGLDDAALRDVAEEKTVFGRITPAQ
                     KESLVKALRADGHYVAMTGDGVNDVLALKQANLAIAMQSGSQVTRGVADIVLLKDSFA
                     ALPAAFLEGQRILKGMQDIIRLFLVRTLYVSLIIFAVSRMGEEFPVTPKQNALLALAT
                     VGLPVLFLAAWARPGPTPRRLVPSSSHFVIPAALTIAGVGTIVYMFFLDMTGELGQAR
                     TALTITTIVCGLALIVFSEPPTPEWTGGDELSGDWRPTILAGVTLLAFCIGLYIKPLR
                     SFYELETMPFTSYLIIGLVVTGWSSILRYLWRLHVVDWSWAHLRDYIVSVRRRLARKK
                     VTVWDEQDANLTE"
     gene            1233..1841
                     /locus_tag="VFV93_13740"
     CDS             1233..1841
                     /locus_tag="VFV93_13740"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX5166459.1"
                     /translation="MSTPQPPADRAPMSEEALQTVTIGGLQPLTAPIRIVDYDPEWPS
                     LFEREAARITALLGDRVVALEHAGSTSVPGLAAKPIIDIVLVVADSADEAVYVPALDA
                     AGYTLRIREPDWFEHRLLKGPDTDINLHVFSAGCPEITRMLCFRDHLRTNDADRQLYE
                     QTKRDLAARDWKYTQNYADAKTAVVEQIIARVGSASSSQGPI"
     gene            1897..>2559
                     /locus_tag="VFV93_13745"
     CDS             1897..>2559
                     /locus_tag="VFV93_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166460.1"
                     /translation="MPPTDRIARAVAAIQADPDLRAYLGMRCGYHPDMLAMSVAGQTV
                     GEVSALYRATLARVVGDEGVSAVDTTVPHLDLVTSDDGIQSIRVVDEAFDIRPMFHGV
                     QLAARAKVAAAASEARLEERILEEVTACLLTETQPPDGPADEFDSWVRSRARASERCT
                     GVVSRLALEFSRSVVGDAVRRADLASASLVDLERMLHLRPPGSVAPQPATPARPLPVD
                     AVF"
BASE COUNT          403 a          861 c          850 g          445 t
ORIGIN      
        1 accctgacgc aatcaaggct gacgatcctt gcctgctgga ggcgatgacg ccgctcgggc
       61 tggtggcgtt cagcgatgag ttgcggcccg aggcgcgaac gacgatcgaa ggcttccgcg
      121 aggccgggat cgctctcaag atcatctccg gcgacaaccc cgatacggtt gcggcgctgg
      181 cgcggcaggc cggattcccg cacgatctgc aagtcgtctc cggcctggaa ctggaggggc
      241 ttgacgacgc ggcgctgcgt gacgtcgccg aggagaaaac cgtcttcggc cgcatcacgc
      301 ccgcccagaa ggagtcgctc gtcaaggcgc tgcgggccga tggccactac gtcgcgatga
      361 cgggcgacgg cgtcaacgac gtgctggcgc tcaagcaggc caatctggcg atcgcgatgc
      421 agtccggcag tcaggttacg cgcggtgtgg ccgacatcgt cctgctcaag gactcgttcg
      481 cggccctgcc ggcggcgttc ctggagggac agcgcatcct gaaggggatg caggacatca
      541 tccggctctt cctcgtccgg acgctctacg tctcgctgat catcttcgcc gtctcgcgga
      601 tgggcgagga gtttccggtc acgccgaagc agaacgcgct gctggcactg gcgacggtcg
      661 gcctgccggt cctcttcctg gcggcctggg cacggccagg cccgactccg aggcggctgg
      721 tgccgtccag cagccacttc gtcatcccgg ccgcgctgac gatcgccggt gttgggacga
      781 tcgtctacat gttcttcctc gacatgaccg gcgagctggg tcaggcgcgg accgcactga
      841 cgatcacgac gatcgtctgc ggcctggcgc tgatcgtctt ctcggagccg ccgacgcccg
      901 aatggaccgg aggcgacgag ctgagtggcg actggcggcc gacgatcctg gccggtgtga
      961 ccttgctcgc cttctgcatc ggcctctaca tcaagccgct gcgcagtttc tacgagctgg
     1021 agacgatgcc gttcacttcg tacctcatca tcggcctggt cgtgaccggc tggtccagca
     1081 tcctgcgcta cctctggcgg ctgcatgtcg ttgactggtc gtgggcgcac ctccgggact
     1141 acatcgtctc cgtccgccgc cggctggccc gcaagaaggt caccgtctgg gacgagcagg
     1201 acgccaacct gacggaatga gagttgtttg acgtgtcgac gccgcaaccg cccgccgacc
     1261 gagcgccgat gtcggaggaa gcgctccaga ccgtgacgat cggcggtttg caaccgctga
     1321 cggccccaat ccggatcgtg gactacgacc ccgaatggcc gagcctcttc gaacgggagg
     1381 cggcgcggat cacggcactg ctcggcgacc gcgtcgttgc gctcgagcac gccggatcga
     1441 cctccgttcc ggggctggcc gccaagccga tcatcgacat cgttctcgtc gtcgctgact
     1501 ccgcggacga ggccgtctac gttccggcgc tggatgccgc cggctacacg ctgcgcatcc
     1561 gcgagccgga ctggttcgag caccggctgc tcaaggggcc ggacaccgat atcaacctgc
     1621 acgtcttctc cgcaggctgc ccggagatca cgcggatgct gtgcttccgc gaccacctgc
     1681 gcaccaacga cgccgaccgg cagctctacg agcaaaccaa gcgcgacctt gccgctcgtg
     1741 actggaaata cacccagaac tacgccgacg cgaagacggc ggtggtcgag cagatcatcg
     1801 cccgcgtcgg cagcgcctcg tcctcacaag ggccgatctg agtccagctg ccgggagtgt
     1861 tggcgcgagg gtgactggct gaaggggagc agttcgttgc caccgaccga tcggatcgcc
     1921 cgtgccgtcg cggcgattca ggccgatccc gatctgcgcg cctatctcgg gatgcgctgc
     1981 ggttaccacc ccgacatgct ggcgatgagc gtggccggcc agacggtcgg cgaggtttcg
     2041 gcgctctacc gggcaacgct cgcccgcgtc gttggcgacg agggtgtcag cgcggtcgat
     2101 acgactgtgc cgcatctcga cctcgtcacc agcgacgacg gaatccagtc gatacgggtg
     2161 gtcgacgagg cgttcgacat caggccgatg ttccacggcg tccagctggc ggcgcgtgca
     2221 aaggttgccg ctgccgccag tgaggcgcga ctggaagaac ggattcttga ggaggtcacc
     2281 gcctgtcttc tcactgaaac ccaaccgccc gacggcccag ccgatgaatt cgacagttgg
     2341 gtccgctctc gggcacgggc gtcggagcgc tgtaccggcg ttgtcagcag gctggcgctc
     2401 gagttctcgc ggtcggtagt cggcgatgct gtccgacgcg ccgatctcgc ctcggccagt
     2461 ttggtcgatc tggagcgcat gcttcacctg cgaccgccgg gttcagtcgc gccgcaacct
     2521 gcaaccccgg cgcgtccgct gccggttgat gctgtcttc
//
LOCUS       DASUGJ010000606         7175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1765791, whole genome shotgun sequence.
ACCESSION   DASUGJ010000606 DASUGJ010000000
VERSION     DASUGJ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765791"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1684
                     /locus_tag="VFV93_13750"
     CDS             146..1684
                     /locus_tag="VFV93_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5166461.1"
                     /translation="MRIGIDVGGTNTDAVLMDGLAVVAKIKTPTTADVTGGITAALRA
                     VLADSGVATGAIRAVMIGTTHFTNAVVERRRLQPAAAVRLGLPATQALPPMTDWPAEL
                     RDAIGNHWFLVHGGHEFDGREISPLDEDEIRGVAAQIAGRGVKAVAVSSVFSPVNDVM
                     ERRAAEIIADVIPDATITCSVEIGRIGLLERENATILNACLRGLATTTIAAFRQAIRE
                     LEIHAPLYLTQNDGTLMSAEFAERYPVLTFASGPTNSMRGAALLCGLADAIVVDIGGT
                     TSDVGALVHGFPREASIVVEVGGVRTNFRMPDVHSFGLGGGSLISDDPLAIGPQSVGY
                     RLTEDALVFGGETLTATDIAVAAGIAGIGDAARVAGLDSDLVSRALDRIHAMTEEGID
                     RMKLAAGDVPVVIVGGGSVLISRSLAGASQIIKPDHFEAANAVGAAIAQISGEVDRVF
                     SLAEMSRAAALDAAKAEATSRAAAAGADAATVDIVEVEDVPLAYLPGNATRIRVKAVG
                     DLAM"
     gene            complement(1767..2285)
                     /locus_tag="VFV93_13755"
     CDS             complement(1767..2285)
                     /locus_tag="VFV93_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166462.1"
                     /translation="MLNSHRRQRTFILVLASLLALFGATTQLASARQATPFHAVLVTI
                     NQPPALCGEAHLCIALDGNGVATHLGRVHETGQVEIDLTSQSGPCTNNVRTMLLTAAN
                     GDQLSLLITGVNCDTGATSGITGISHDTWVVTGGTGRFSGATGSGIETVYVNLPASTA
                     FTYMSGTLSLSH"
     gene            complement(2567..4069)
                     /locus_tag="VFV93_13760"
     CDS             complement(2567..4069)
                     /locus_tag="VFV93_13760"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEX5166463.1"
                     /translation="MNRDMNAFRRPPTTPLGRVLHDIARHDVPDDSDLRDRVMNAIKR
                     HEADRAPRDAAGRAWNLPALDPPRRRRRVTDSNEEHESTIVSSQPNLEPIPVERHRWL
                     REGLKLAAAAIIFGIIGALLVLVLRNNDSGDHSSVPGAGPSEATATTLVSPTISATVV
                     TQVSPTIDATVARQSTRQASQDAARQALTATADSATATQSALPSPGEVVATIELGESP
                     GWFATTDGALWVGNTGEGTISRIDPTTNTVVATVTVAPRGGRGGDAVPNWLVSYDGQV
                     WATDNPENKIVRVDPATNTVVQAITMVVANESDRPKPGGLIVDSTGVWASDIDGGRLV
                     HFDAVTGAVLAEIPMDQPSSLGSGFGSIWVVAGQGAQTIVRVDPSTHTIVSEIPVTSN
                     VWVFEILDDAVWGIGGDGDLFRIDPATNTLVATIDTGLPSSHASHVTPAGIWVGGYLH
                     GGLVRIDPDTGAQSRNINDDHIVWDLIEFDGSIWAANGNANTVVRIDPAP"
     gene            complement(4066..4704)
                     /locus_tag="VFV93_13765"
     CDS             complement(4066..4704)
                     /locus_tag="VFV93_13765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5166464.1"
                     /translation="MSLDERTAINQMQRGDIRGLDALMRAYQVQAIRAAYLVTHDLPL
                     AEDIVQSAFIRAYERIGQYDSSRPFGPWFMRSVVNSAVTAASRRKRHVPLVGAGSVDA
                     FGADEFADPESGPEARMLGAETRAEVWQAIDSLPPSQRAAIVLRYYLELPEAETAERL
                     GIPAGTVKSRLSTARGRLRTVFGSQDPTNHDYQPVSSSRSTTHGELDEEAER"
     gene            complement(4826..5449)
                     /locus_tag="VFV93_13770"
     CDS             complement(4826..5449)
                     /locus_tag="VFV93_13770"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5166465.1"
                     /translation="MNSKRIVQDGYDILAETYLRVRSNDREDVRLLAELAQRLPDGAK
                     VLDAGCGAGVPVAQILSERCRVTGVDISAAQIALARELVPAAEFIQADMAGLDFPDGS
                     FDAIVSYFAIIHVPREEHPAILANFHRMLAPGGLLLLSTGAGDNPDDTEDNWLDGGAT
                     MYWSHYGHADNLRLIAEADFDIILERLVTEDEEFGGGTHLFVLAQAR"
     gene            complement(5446..5895)
                     /locus_tag="VFV93_13775"
     CDS             complement(5446..5895)
                     /locus_tag="VFV93_13775"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEX5166466.1"
                     /translation="MPYFAYCTLLDKGEMQRFVPAASRGATGQISGWRVVFERHAPGV
                     ETGGCNLSPADDAPVYGVLYDLSEDGFAQLDAISGVDRGLYQRFAVRVMTDAGEVTAE
                     TYVIPNPGGPFQPTAAYVRPILAGARELGLPNDYVATLDALVRESLA"
     gene            complement(6385..6876)
                     /locus_tag="VFV93_13780"
     CDS             complement(6385..6876)
                     /locus_tag="VFV93_13780"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX5166467.1"
                     /translation="MLENDERVDLPAYRAAFLRHRPNDPPERIGGATADAFGNTPEMA
                     DGLAALVCSGVKTATSGLLWGYEAEGEPIPAAGDLSIILDGRGAPVCIIENLETRIIP
                     FDAVDEQLAWEYGEGERTLAWWREHLWDYYAAECAESGWKPSLEMPLVVRRFQAVYPA
                     EQQ"
BASE COUNT         1206 a         2278 c         2332 g         1359 t
ORIGIN      
        1 agcgaaggac ctcccctgtc ttgaccaccc gtgcgtataa cgcggagagg tccttcgctg
       61 cggctcagga cgacagggga actccgatgc ctctcgttac tggtgacgag gatgacatgg
      121 tagccgtccg gttgagaggg acgtaatgcg aatcgggatc gacgttggcg ggacgaatac
      181 ggacgcggtc ctgatggacg gccttgccgt cgtcgccaag atcaagacgc caacgacggc
      241 cgatgtcacg ggcgggatca cggccgcgct gcgtgcggtg ctggctgact ccggcgtggc
      301 gacgggcgcg atccgggcgg tcatgatcgg gacgacgcat ttcacgaacg cagtcgtcga
      361 gcgccgaagg ttgcagccgg ctgccgccgt tcggctcggc ctgccggcga cgcaggcgct
      421 gccgccgatg accgattggc cggccgagtt gcgcgatgcc atcggcaacc actggttcct
      481 cgtccacggc ggccacgagt tcgatggccg cgagatctcg ccgctggatg aggatgagat
      541 acggggcgtc gctgcccaga tcgctggccg cggtgtcaag gccgtcgctg tctcgtcggt
      601 cttctcgccg gtcaacgacg tcatggagcg gcgggcggcg gagatcatcg ccgacgtcat
      661 tcccgatgcc acgatcacct gctcggtcga gatcggccgg atcgggctgc tggagcgcga
      721 gaacgcgacg atcctcaacg cctgcctgcg cggactggcg acgacgacga ttgccgcctt
      781 ccgccaggcg atccgcgaac tggagatcca cgccccgctc tacctgaccc agaacgacgg
      841 cacgctgatg agtgccgagt tcgccgagcg gtacccggtc ctgaccttcg cgtccggccc
      901 gaccaactcg atgcgtggcg cggcgctgct ctgcggcctg gctgatgcga tcgtcgtcga
      961 tatcggcggg acgacctccg atgtcggcgc gctggtgcac ggattccccc gcgaggcgtc
     1021 gatcgtcgtc gaggttggcg gggtgcgcac caacttccgg atgccggatg tccactcgtt
     1081 cggacttggc ggcggctcgc tcatcagcga cgacccgctg gcgatcggcc cgcagtcggt
     1141 cggctaccgg ctgacggagg atgcactcgt cttcggtggc gagacgctga cggcgaccga
     1201 catcgccgtc gcggctggaa tcgccggcat cggtgacgcc gcgcgcgtcg cgggtctcga
     1261 cagcgatctg gtctcgcggg cgctcgaccg catccacgcg atgacggagg aggggatcga
     1321 ccggatgaag ctggcagccg gcgacgtgcc ggtcgtcatc gtcggcggcg gctcggtcct
     1381 gatctcccgc tcgctggccg gcgcctcaca gatcatcaag ccggaccact tcgaggctgc
     1441 caacgccgtc ggtgccgcca tcgcccagat ctccggcgag gtcgatcgcg tcttctcgct
     1501 ggcggagatg agccgcgccg cggccctcga cgccgcgaaa gccgaggcaa cctctcgcgc
     1561 ggccgccgcc ggagcagatg cggcgaccgt cgacatcgtc gaggtcgagg acgtgccgct
     1621 ggcctatctg cccggcaacg cgacccgaat ccgcgtcaaa gccgtcggag atctcgcgat
     1681 gtgagatgag tgctgacgtt taaatttgat ccgcgtcgac agcgtcggtg agagtctcac
     1741 cgacgctgtt tcatcgcagc gctgcgctag tgggagagcg acagtgtgcc gctcatgtac
     1801 gtgaaggccg tcgacgccgg caggttgacg tacacggttt ctattccgct accggtcgcg
     1861 ccgctgaagc gacccgtccc gccggtcacg acccaggtgt cgtgtgagat gccggtgatc
     1921 ccggatgttg ctccggtgtc gcagttgacg cctgtgatca gcagcgagag ttggtcacca
     1981 ttggcggcgg tgaggagcat cgtccggacg ttgttggtgc acgggccgga ctgactggtc
     2041 agatcgatct caacctggcc ggtctcatgc actcggccga ggtgagtggc gacgccattg
     2101 ccatccaacg cgatgcagag gtgtgcttct ccgcacagag ccggtggctg attaatcgtc
     2161 accagcacgg catgaaacgg tgtcgcttgc ctggctgagg cgagttgggt cgtcgctcca
     2221 aacaacgcca gcaggctggc gaggaccagg atgaaggttc gctgtcgacg atgcgagttg
     2281 agcatgaagc atcctttcat cgagaggttc cgcagcgaca cagagcgtca cgaagaactc
     2341 gccagtggct gcctcctctc cggacagccg cgctcgcacc aagcccacta tgggacggag
     2401 aacgcagcgt cgtcgtcaac cggacacgcg tctgagggtg actctaaaag acatactcac
     2461 gagcacacgg atcggttcgt tctctaccca accgatccgt gtgctcaggt tgactgcgta
     2521 gtcgattcag gattctggtg cggagttgcg ccgctgcctg acgagtttaa ggcgccgggt
     2581 cgatgcgcac gacggtgttg gcgttgccat tcgccgccca gatcgagccg tcgaattcga
     2641 tgagatccca gacgatgtgg tcatcgttga tgttccggct ctgtgcgccc gtatctggat
     2701 cgatccgcac cagtccgccg tggagatagc cgccgaccca gatgccggcc ggtgtcacgt
     2761 gtgaggcatg cgaacttggt agtccggtat cgatggttgc taccagcgtg ttggtagcgg
     2821 gatcgatgcg gaagaggtcg ccgtcccctc caatacccca gaccgcgtcg tccaggatct
     2881 cgaagaccca gacgttcgac gtgactggaa tctcactcac gatcgtgtgc gtcgacggat
     2941 cgacccggac gatcgtctgg gcgccttgcc ccgccacgac ccagatcgat ccaaatccgg
     3001 acccgagcga gcttggctga tccatgggta tctccgccag gacggccccc gtcacggcat
     3061 cgaagtgaac cagacgcccg ccatcgatgt ccgacgccca gacaccggta ctgtctacga
     3121 ttaacccgcc tggcttgggg cgatcgctct cattggcgac gaccatcgta atcgcctgca
     3181 cgaccgtgtt cgtggcgggg tcaacccgca cgatcttgtt ctcgggattg tcagtcgccc
     3241 agacctgccc atcgtagctc accagccagt tcggcacggc atcgccgcct ctgccgccac
     3301 gtggggcgac ggtcaccgtc gcgacaacgg tgttcgtcgt cggatcgatg cgcgagatcg
     3361 ttccctcgcc ggtgttgcca acccacagcg caccgtcggt ggtggcgaac cagccgggac
     3421 tctcgccaag ttcgatcgtt gcgacaacct caccgggaga tggcagagcg ctttgcgttg
     3481 ctgtcgcgct gtccgcggtg gccgtcagtg cttgcctggc agcgtcctgg gatgcctgtc
     3541 gggtggactg ccgggcgacg gtcgcgtcga tggttggact gacctgcgtc accacggtcg
     3601 cgcttatcgt tggactgacc agtgtcgttg cggttgcctc tgacggccct gcgcctggga
     3661 cacttgaatg gtcgccggag tcgttgttac gcagcacgag aacgagaagg gcgccaatga
     3721 tcccgaagat gatcgcggct gccgcaagct tgagtccctc acgtagccaa cggtgccgct
     3781 caactggaat tggttcgagg tttggttgcg aagacacgat ggttgactcg tgttcctcgt
     3841 tggagtcggt gacccgtcgc cgtcgccgag gcgggtcgag cgcaggtagg ttccaggctc
     3901 gccctgccgc atcgcgcggc gctcggtctg cctcatgtcg tttgatcgcg ttcatcaccc
     3961 ggtcacgcag gtcggagtca tccggtacgt cgtggcgcgc gatgtcatgc aggacgcgtc
     4021 cgagtggtgt cgttggtggt cgtcggaagg cgttcatgtc gcggttcatc gctccgcctc
     4081 ctcatccagc tcaccgtggg tggtgctgcg tgatgacgag acgggctggt aatcatggtt
     4141 ggtgggatcc tggctcccga acaccgtgcg tagccggcca cgcgctgtgg acaggcggga
     4201 cttcactgtc ccggctggaa taccgagccg ctcggcggtc tctgcttcgg gcagttccag
     4261 gtagtagcgc agcacgatcg cagcgcgttg cgagggcggc aggctgtcga tcgcctgcca
     4321 gacctcggca cgggtctctg ctcccagcat ccgtgcttca ggaccgctct ccggatcggc
     4381 gaattcatct gctccgaacg cgtcgacaga gcctgcgccg accaagggta cgtgtcgctt
     4441 ccgacggctc gcggccgtca cggcgctgtt gacaacactg cgcatgaacc acggaccgaa
     4501 tggccgtgag ctgtcgtact gaccgatccg ctcgtacgcg cggatgaagg ccgattgcac
     4561 gatgtcctcg gccagcggga gatcgtgagt gacgagatag gcagcccgga tcgcctggac
     4621 ctggtaggca cgcatgagcg catccagccc gcggatgtcg ccgcgttgca tctggttgat
     4681 ggctgtgcgc tcatccagcg acatcgtgcg ccccatctgc cgccacgctt gattcccact
     4741 gtcgtatccg tctgtgagta ctactcgcca gcacgggagt tggttcagtt gaagtgacat
     4801 cagccgggtg atggcttgcg gttagctatc gtgcttgcgc caggacgaac agatgtgtcc
     4861 cgccgccgaa ctcctcgtcc tcagtcacga gccgctccaa gatgatgtcg aagtccgcct
     4921 cagcgatcag ccgcaagttg tcggcgtggc cgtagtggct ccagtacatc gtcgcgccac
     4981 cgtcgagcca gttgtcctcg gtgtcgtccg ggttgtcgcc ggcaccggtt gacagcagga
     5041 gcagcccgcc cggtgccagc attctgtgga agttggccag tatcgccgga tgctcctcgc
     5101 gcggaacgtg gatgatcgcg aagtaggaga cgatcgcgtc gaagctgccg tcggggaagt
     5161 cgagcccggc catatcggcc tggatgaact cggcagccgg caccagttcc cgcgccagtg
     5221 cgatctgcgc ggctgagatg tcgacgccgg tgactcggca gcgctcgctg aggatctggg
     5281 cgaccggcac cccggccccg caaccggcgt cgagcacctt cgcgccgtcc ggcagccgct
     5341 gcgctagctc tgccagcagg cgcacgtcct cacggtcgtt gctccggaca cgcaggtagg
     5401 tctccgccag aatgtcgtaa ccgtcttgga cgatccgctt gctgttcacg ccagtgactc
     5461 gcggaccagc gcgtcgaggg ttgcgacgta gtcgttcggc aggccaagct cccgcgcgcc
     5521 ggcgaggatc gggcgaacgt aggcggctgt cggctggaac gggccaccgg gattcgggat
     5581 gacgtacgtc tcggcggtaa cctcacctgc gtctgtcatc acccgaacgg caaagcgttg
     5641 gtagaggcca cgatcaaccc ccgagatcgc gtcgagttgc gcgaacccgt cctcgctcag
     5701 atcgtagagg acaccgtaaa ccggcgcgtc atcggcaggc gacaggttgc agccgcctgt
     5761 ctccacacct ggcgcgtgtc gctcgaaaac gacgcgccag ccgctgatct ggcctgtcgc
     5821 accacgactc gcagctggca cgaagcgctg catctcgccc ttgtccagta gcgtgcagta
     5881 ggcgaaatac ggcataccgt gcgtccttcc cgataagcat tctcgatcgt aacagcctag
     5941 cacgattgcc ggtctcactg agtcccggaa ggcgtgagat gtatcgcggc ggcacgcaca
     6001 acgatagcga cactggacat gtcatcgaag ttggttgagt aagatcctga cgctcatgct
     6061 tccatgatcc gctacctgcc ctgacgatca atcgaggcag gaggttcgcc atgctctgaa
     6121 cgcgcttcac tgatcttttt ggctgcgacg tgccaatcca gcaggacggc atgggttcgc
     6181 tcgcggcgcc tccaggcgca aaggtggtag ccgatgcagg cggtttggga actgtctcgg
     6241 tcgagccatc tggcagaaat caatggagaa gccgcgatgc tccccggtgt cagcgatcac
     6301 ctcgatgcgg tggtgctcgg cgttggttag attgtcatcc cccttggtgt cgacgtcgaa
     6361 gcggaaacga caacggcggg tagatcactg ctgctcggct ggatacaccg cctggaatcg
     6421 cctcacgacc agtggcatct ccaacgacgg cttccaaccg ctttcggcgc actcggcggc
     6481 gtaatagtcc cacaggtgct cgcgccacca ggcgagcgtg cgctcgccct caccgtattc
     6541 ccaggcaagc tgctcgtcga cggcgtcaaa gggaatgatg cgcgtctcga ggttctcgat
     6601 gatgcaaaca ggtgcaccgc gtccgtcgag gatgatcgac aggtcgcctg ccgctggtat
     6661 cggctcacct tcagcctcgt agccccacag gagccccgag gtcgccgtct tcacgccact
     6721 gcacaccaat gccgcgagtc cgtctgccat ctcgggggtg ttcccgaagg catcagcggt
     6781 tgcgccgccg atccgttcgg gtgggtcgtt cggccgatgt cgcaggaacg cggcccgata
     6841 cgccgggaga tccacgcgct cgtcattctc cagcatgtgc tcgcaacctt tcctcaagac
     6901 cttgctggtg cccactacat catcggggag acacggatgt ctccctgctt gagctgaatt
     6961 attgcggtgc gctcgtcgcc ggccatgccg acttacctca ccgatccgat gcactgctct
     7021 gatgtgtgtc cctactggag ctactcagta gtcaggtgaa aggttcggtt gatgaagagg
     7081 gagatgtcgc acatccgcgc gggaccgtcg atgccagttc gcgtagcata gagcgtgtta
     7141 tggacttgcg gcgaccaaca gtttcatgac gcggg
//
LOCUS       DASUGJ010000607         9301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1348711, whole genome shotgun sequence.
ACCESSION   DASUGJ010000607 DASUGJ010000000
VERSION     DASUGJ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348711"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFV93_13785"
     CDS             complement(<1..513)
                     /locus_tag="VFV93_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HEX5166468.1"
                     /translation="MFCSSTVGSITRRSLWLAWWSGHSHGRYAGSTWYADEFALDLME
                     HCVSHINGDSPGCRWATVYDNVMRVEAAAALVSQIVKDVTGQESTGLRPLRAGDNSFN
                     NIGIPLYFMLSSTMPEDLRLEKGYYGVAGCGGNIAWHTEDDTLEIADRDNLRRDIALY
                     ATAVWRVANAP"
     gene            482..655
                     /locus_tag="VFV93_13790"
     CDS             482..655
                     /locus_tag="VFV93_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166469.1"
                     /translation="MIEPTVDEQNILEETLFDEAEFLKTFGLDRFLLDLRGQQLKIKD
                     MFHRSHRSWRRLC"
     gene            complement(679..2430)
                     /locus_tag="VFV93_13795"
     CDS             complement(679..2430)
                     /locus_tag="VFV93_13795"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5166470.1"
                     /translation="MPIDDVLADLSVDRAWEHLVHITEEIPTRLAGSANSRRMAEYAG
                     EQLARNGLSPTMHEFLGLVSFPESAEVRVLGQGARIIEANTLGHSASTTGIEGELVYV
                     GSGAESDYEGKDVRGKITLSELSYSPARHEKALIAWQKGSTAQIMMNWGGPDNEAIPF
                     GSMKSAWGNPTPETLATEMPDLPCVGISRVEGLRLKDASLAGTVRVWLNAKADNGWKP
                     LTMTTGEFDAERQFLLVGGHMDSWFGPQATDNAAGSACILELARAFAAHGDQLRRGIV
                     TGFWMGHETGTMISSSRFADLEWDRLRRDCVAYLQIDQPAITGSSTWHLHSTDDIQSW
                     AMRTTQSVLGDMQIHWRRQQKNGDSSFFGVGLPCIAGEMSFTDEEIQATALATLGWWH
                     HSLHNTIDKVDKERLALHLRVYAHWMWELLTAPVLPYEYEPVAQRFVDRLSELARTPV
                     SGIDMAGAVERARAFQVVAGRLDAAGRDWSARTGSNDLDRAAGALNRAMISLSRTLVP
                     VASTVVGSYGQDRYGHAWQTQMIPSLVPYPRLASLPTDSEEWQTSWVAMVRARNRVVD
                     ALDRATEIAGAAVDQVG"
     gene            complement(2476..3768)
                     /locus_tag="VFV93_13800"
     CDS             complement(2476..3768)
                     /locus_tag="VFV93_13800"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5166471.1"
                     /translation="MPGVTALHAHRLIDGTGRPPIDDGVVVVEAERILAVGPAGSTSI
                     PPDAERIDLGDRTLLPGFIDAHSHATINPGLRGIIGQLEGLNEPSPRQVLRGARNLRI
                     DLASGVTTMRLVGEVPYNDVTLRDAIGAGYIVGPRLLISGRAITSSNGHGSLVGAWIV
                     DGEDEMRRVVREQLRMGCDFTKLIVTGRRYVKNAKEYLGFTEAEIAAAIDETHRVGSR
                     IGAHLRMDLETGLRLCLQHGLDLTDHVFPVDSESIDLFLSSGATAVPTYTIALQTQVD
                     WNQLHGKPIADRLAHIRQLDAERLAQREFDDRAVEARVTLVRDHAADAFRRAAAAGVK
                     SCLGTDAMHGLFAYEMEILAGWDIPEMDAIVAGTLSGAKALGIDEETGSLEAGKLADI
                     IAVDGDPLADIRALGNVSFVMRGGRRYDPAALLEDIAV"
     gene            complement(3996..4871)
                     /locus_tag="VFV93_13805"
     CDS             complement(3996..4871)
                     /locus_tag="VFV93_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5166472.1"
                     /translation="MPHAQVNGVTLYYEETGDGPPIVFCHEFAGDYRSWQPQVEAFSA
                     TNRCIVYSQRGYPPSDVPSDIDAYTQELLVEDLRGLLDHLGIDRAHIVGLSMGGNVAL
                     NFALAHPDRCLTTVVAGCGSGTVGREQFERDNDRVVAYLREHGMAAFAEMYAEGAARV
                     TFKRKDRAGWELFRRQMAEHSATGSAFAMAGVIRRRPTIFALKERLNQLRVPTLLVVG
                     DEDEPCIEPGIFMKREIATAGLVILPQSGHTINLEEPARFNQAVADFHAAVAAGDWAT
                     REEVTVSWLLPEARA"
     gene            5250..7394
                     /locus_tag="VFV93_13810"
     CDS             5250..7394
                     /locus_tag="VFV93_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5166473.1"
                     /translation="MTNPDDVLERISDALYLLNSNWCFTYLNDRACAMLRHSREELLG
                     RNVWHAFPDAIDTVFDIEYHRALETGNPVTFEVNYPRLGGWFEIRVYPSREGLTVFFQ
                     EINERVERESALRESEARYRRLIDHLPAVTYSRTTDSATREVHVSPQIEAMTGYPVEE
                     WHATSQLWVSSIHPDDRALVAAELERSDATSEPFRAEYRLVRKDGRVIWVRNEAVLIA
                     DQDPMPPAWQGFMLDITERKQLEEQLLHAAFHDFLTGLPNRAHFLDRTNHALARANRM
                     ETAIAVLFLDLDNFKDINDSYGHALGDELLTRVAQVLRLRVREGELLTRLGGDEFAVL
                     LEDTGDIDSATRVAERLLEALHEPLLLSGRKVFVSASAGIVTSAPRHATPGDLLRDAD
                     IALYRAKDAGKRQFAIFEEAMHADVTDRLELESDLRRALVNDEFVLEYQPKVSLTTRA
                     IVGVEALIRWEHPTRGRISPATFIPIAEQSGLILPIGRWILREACTQAAGWSHVYDGD
                     GPFVVSVNLSGRQFGDPHLVEHVSSILHATGLDPAYLGLEITESAVMDDVELACSRLQ
                     ELKRLGVRLGVDDFGTGYSSLAYLKRFPIDVLKIDRSFVNGLESSAQDRAIVAATITL
                     AHALGLRVIAEGIETEAQLTELRRLGCDAAQGFLFSRPVTPSTITSMIVQAHPLPIAA
                     TAGSSRTSSGRTPRRSIPRRRRPADHARRRRS"
     gene            complement(7489..8544)
                     /locus_tag="VFV93_13815"
     CDS             complement(7489..8544)
                     /locus_tag="VFV93_13815"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEX5166474.1"
                     /translation="METVELLKTLSDVFGPSGFEDEVCQTLQRLIAPFVDEMRVDTLG
                     NLIATRRGTGDTTLMLDAHMDEIGFIVSFVEEGGFLRFTQTSGWDPRIVPAHAVTIRT
                     DHGTRVKGYIGMPPPHILRPEDRDKPYKLEDLFIDVGATSVEEVANLGIRVGSPLVIA
                     YPFERLNEQMVMARAIDDRAGCAVIVKVLEALAGQELESTVVACFSVQEEVGLRGAGT
                     AAYQIEPDIALVLETTVAADVPGIPPARQPTRFGRGPAVVVMDNTMITAVGVVRAITE
                     LAEEHLIPWQYRVPFGGGTNAGAIQRSRGGVQTGVVSLPVRYFHSPYAIMRLDDFEHT
                     VRLVTEFTRSCPGIVSR"
     gene            complement(8551..>9301)
                     /locus_tag="VFV93_13820"
     CDS             complement(8551..>9301)
                     /locus_tag="VFV93_13820"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5166475.1"
                     /translation="PLRIPEPRGTFLYAEYPRPVAACAAEVSQRIAESVFGAMAQVLP
                     ERIFAAPAGTSGNFSLGGYDPATGRRYIMYVFSGGGYGGWWGGDGLTNGCSTIGISKT
                     QPIEILEQHYPVCFDEYALREGSAGAGKHRGGFGVNYRARLMRGEATASFVMEHGRTP
                     PHGLFGGLPGAMNEIEVSQSGAVTRPEHLSKGEGYVLQPGDWVQVRTPGGGGYGDPAE
                     RDPNLVERDLKRGYFAAEDAAPCGFSRGNGR"
BASE COUNT         1647 a         3022 c         2945 g         1687 t
ORIGIN      
        1 gggtgcattg gccacgcgcc agaccgccgt cgcatagagg gcgatgtccc gccgcaaatt
       61 gtcgcgatcg gcgatctcga gcgtgtcgtc ttcggtgtgc caggcgatgt tgccgccgca
      121 gccggcgacg ccgtagtaac ccttctccag ccgcagatct tccggcatcg tcgacgagag
      181 catgaagtag agcgggatgc cgatgttgtt gaacgaattg tcgccggccc gcagcggccg
      241 caacccggtt gattcctgtc cggtgacatc cttgacgatc tggctgacca gggcagcggc
      301 cgcttcgacc cgcatcacgt tgtcatagac ggtcgcccag cggcagccgg gcgagtcacc
      361 gttgatgtgg gagacgcagt gctccattag gtcgagggcg aactcgtccg cgtaccaggt
      421 cgagcctgcg tagcgtccat gtgaatgacc gctccaccac gccagccaga gcgaccgccg
      481 tgtgatcgag ccgacggtcg acgagcagaa catcctcgaa gagacgctgt tcgacgaggc
      541 tgaattcctg aagacgttcg ggctggaccg tttcctgctg gacctgcgtg gccagcaact
      601 caagatcaag gacatgttcc atcgttccca ccgaagctgg cggcggctgt gttgaactga
      661 tgggctagcc gcagaccgtt accccacctg gtcaacggcc gcgccggcaa tttccgtcgc
      721 ccggtcgagc gcatcgacga cccgattacg ggcacggacc atcgcgaccc acgacgtctg
      781 ccattcctcg ctgtcggtag ggagggatgc cagccgtggg tacggcacga gagaggggat
      841 catctgcgtc tgccatgcgt gaccatagcg atcctggccg tatgagccga cgaccgttga
      901 tgcgacaggc accagcgtcc gcgacaggct gatcatcgcc cggttgaggg cgccggcggc
      961 gcggtccaga tcgttcgatc ccgtccgggc actccagtcg cgtccggccg cgtcgagtcg
     1021 gcccgcaacg acctggaatg ccctcgcgcg ctcgacggcg ccggccatgt cgataccgga
     1081 cacgggagtc cgtgccagct cactcagccg gtcgacgaag cgttgggcga ccggctcgta
     1141 ctcgtacggt agcaccggcg ccgtcagcaa ctcccacatc cagtgggcgt agacgcgcag
     1201 atggagcgcg agccgttcct tgtcgacctt gtcgatcgtg ttgtggagcg agtgatgcca
     1261 ccagccaagc gtcgccaggg cggtcgcctg aatctcttcg tccgtgaatg acatctcgcc
     1321 cgcgatgcag ggaagaccga cgccgaagaa ggacgagtcg ccattcttct gttgacgccg
     1381 ccagtggatc tgcatgtcac caagaaccga ctgcgtcgtc cgcatcgccc acgattggat
     1441 gtcgtcggtc gagtggaggt gccaggtcga ggagccggtg atggccggct ggtcgatttg
     1501 caggtaggcg acacagtccc gacgcagccg gtcccattcg aggtcggcga agcgtgagga
     1561 ggagatcatc gtcccggtct cgtggcccat ccagaagccg gtcacgatcc cacgccgcaa
     1621 ctggtcgccg tgggcggcga acgcccgggc cagctccagg atgcaggcgc tgccagcggc
     1681 gttgtcggtt gcctgcgggc cgaaccacga gtccatgtgg ccgccgacga gcaggaactg
     1741 ccgctcggcg tcgaactcac cggtcgtcat cgttagcggc ttccagccgt tgtccgcctt
     1801 cgcgttgagc cagacccgga ccgttcctgc caggctggca tccttcagcc gcaatccctc
     1861 gacgcgggag atgccgacac agggcaggtc gggcatctcg gtcgccagtg tctccggtgt
     1921 ggggttcccc cacgccgact tcatcgaccc gaacgggatg gcctcgttgt ctggcccgcc
     1981 ccagttcatc atgatctgcg cggtcgaacc cttctgccag gcaatcagcg ccttctcgtg
     2041 ccgggcgggc gagtaggaca gctccgagag cgtgatcttg ccccggacgt ccttaccctc
     2101 atagtccgat tcagcaccgg aaccgacata gacgagttca ccttcgatac cggtggtcga
     2161 cgctgaatga ccgagcgtgt ttgcctcgat gatgcgagcg ccctgcccga gcacacgcac
     2221 ctcagccgac tccgggaacg agacgagacc gaggaactcg tgcattgtcg ggctgagccc
     2281 gtttcgtgcg agttgctcgc ctgcgtattc ggccatccgg cgcgagttgg ccgagccggc
     2341 gagcctggtt ggaatctctt cggtgatgtg gaccaggtgc tcccaggccc gatcgaccga
     2401 caggtcggct agcacgtcat caatcggcat cttcctcacc cttcgtatcg atcgtacgac
     2461 gtctatcggc cggtgtcaga cagcgatgtc ttcgagcagc gcggccggat cgtaccggcg
     2521 tccgccacgc atcacgaacg acacgttgcc gagcgcccgg atatcagcca gcgggtcgcc
     2581 atcgaccgcg atgatgtcag ccagcttgcc cgcctccagt gagccggtct cctcgtcgat
     2641 accgagtgcc ttcgcgccac tcagcgtccc ggcgacgatg gcgtccatct ccgggatgtc
     2701 ccaaccggcg agaatctcca tctcgtaggc gaacaacccg tgcatcgcgt cggttccgag
     2761 acaggacttg acaccggccg ccgcggcacg gcgaaacgca tccgccgcgt ggtcacggac
     2821 gagggtgacg cgcgcttcga cagccctgtc gtcaaactcg cgctgtgcca accgctcggc
     2881 gtcgagctgg cggatgtggg cgaggcgatc ggcgattggc ttgccgtgca actggttcca
     2941 gtcgacctgc gtctggagcg cgatcgtgta ggtcgggacg gcggtcgctc ccgacgagag
     3001 gaacaggtcg atcgactcac tgtcgaccgg gaagacgtga tcggtcaggt cgagcccatg
     3061 ttgcaggcag agccgcagtc ctgtctcgag gtccatccgc agatgcgcgc cgatcctgct
     3121 accgacacga tgcgtctcat cgatcgccgc cgcgatctca gcctcggtaa acccgagata
     3181 ctccttcgcg ttcttgacgt accgccggcc ggtcacgatc agtttcgtga agtcgcagcc
     3241 cattcgcaac tgctcgcgga cgacgcggcg catctcgtct tcgccatcga cgatccacgc
     3301 gccaaccagc gaaccatggc cgttggacga cgtgatcgct ctcccgctga tgagcaggcg
     3361 cggcccgacg atgtaccccg ccccgatcgc gtcacgaagt gtgacatcgt tgtacggaac
     3421 ctcaccgacc aaccgcatcg tcgtcacgcc ggacgcgagg tcaattcgca gattgcgcgc
     3481 gccgcgcagc acctgccgtg ggctcggctc gttcaaccct tcgagttgcc cgatgatgcc
     3541 gcgcaggccc ggattgatcg ttgcgtgact gtgggcgtcg atgaagcctg gtaacaacgt
     3601 tcggtcgcca aggtcgatgc gctcggcgtc gggtgggatc gatgtcgagc cggccggccc
     3661 gacggccagg atccgctccg cctcgacgac caccacgcca tcgtcgatgg gtggccgtcc
     3721 ggttccgtcg atcaggcggt gagcgtggag agcggtcaca ccaggcatta gcttctcctc
     3781 gatgtctcag caacccgcgg cggcagtgac gacagcatct gtccttcaac gagcgaaccg
     3841 gtcgtgcgcg ttatgacaag catggtatgc ggaacgttgc gagcgggcaa tgccctatcg
     3901 agcgccgatc gtcgctcagt gcaatcgagt cgaatgaagt gccgtgcgtc attctacccg
     3961 ccggatgtcc acgtcgaggc gctcggcgga ccgggtcacg cacgcgcctc aggcaggagc
     4021 cagctgaccg tgacctcctc gcgcgtcgcc cagtcacccg ctgcgaccgc cgcgtggaag
     4081 tcagcgaccg cctggttgaa gcgcgccggc tcttcgaggt tgatcgtgtg cccgctctgc
     4141 ggcaggatga ccagaccggc agtcgcgatc tcccgcttca tgaagatgcc cggctcgatg
     4201 cacggctcat cctcgtcgcc aacgacgagc aaggtgggga cgcgcaattg gttcaggcgc
     4261 tccttgagcg cgaagatcgt cgggcggcgg cggatgaccc cggccatcgc gaacgcggag
     4321 ccggtggccg aatgctcggc catctgccgg cgaaagagct cccagccggc gcggtccttg
     4381 cgcttgaagg tgacccgcgc cgcgccctcc gcatacatct cggcgaaggc ggccatgccg
     4441 tgttcccgca ggtacgcgac gacccggtcg ttgtcacgct cgaactgttc gcgtccgacc
     4501 gtccccgagc cgcagccggc cacgaccgtc gtcagacagc ggtccggatg ggccaaggcg
     4561 aagttcagcg cgacgttgcc acccatcgag agcccgacga tgtgcgcgcg gtcgataccc
     4621 aggtggtcca gcagcccgcg cagatcctcg accagcagtt cctgcgtgta ggcgtcgatg
     4681 tccgacggga catcggaggg cgggtagccg cgctggctgt agacgatgca gcggttggtg
     4741 gctgagaagg cctcgacctg cggctgccag ctgcggtagt cgccagcgaa ctcgtggcag
     4801 aaaacgatcg gcggcccgtc gccggtctcc tcgtagtaca gcgtgacgcc attcacctgt
     4861 gcatgtggca tgttgcctcc atcccggaca aggctgatcg accgagtctc tcacatcttg
     4921 agacatccag atactccgga tcgtgcgtca ggcgatgcag ttcgtaaaac gtacaggcgg
     4981 aaccgctgac ccttgcggct ccgcttccat gtgactggcc tggaggcgag ttgaatcgat
     5041 gtcggcagca agcggtagag ccatccttca ctctcggcat agcaattgcg caagaacagg
     5101 acgatcttcc tgtgcaggag gttgttggtc cagtgtaggc ttgaatctca caacggcgta
     5161 gattcgggaa attcgtcgtt tccgacaggc gtaggtagat cgatcagatc atgaagtatg
     5221 tgttgacgga cgctcagcct acgttgtcca tgacgaaccc ggacgacgtc ctcgagcgta
     5281 tcagtgatgc actgtacctg ctcaattcca actggtgctt cacctacctc aacgaccgtg
     5341 cctgcgcaat gctgcgtcac tcccgtgagg aacttctcgg caggaacgtc tggcatgcat
     5401 ttcccgacgc gatcgacacc gtgttcgaca tcgagtatca ccgtgcgctt gagactggga
     5461 atcccgttac attcgaagtc aactaccccc ggctcggtgg ctggttcgag atccgcgtct
     5521 atccctctcg tgaaggacta acggtcttct tccaggagat caacgagcgc gttgaacgcg
     5581 agagcgcgct ccgcgagagc gaagcgcgct atcggcgatt gatcgatcat cttccagccg
     5641 tgacgtattc caggacgacc gactccgcaa cgagggaagt tcacgtcagc ccacaaatcg
     5701 aagcgatgac cggttatccc gtcgaagagt ggcatgccac ttcgcagctc tgggtttcct
     5761 cgatccatcc ggacgatcga gcgctggtgg cagccgagct cgaacgttcc gatgcaacga
     5821 gcgaaccgtt ccgcgctgag taccgcctcg ttcgcaagga tgggcgggtg atctgggttc
     5881 gcaatgaggc agttctgatc gcggatcagg atccgatgcc gcctgcctgg cagggcttca
     5941 tgctggatat caccgagcga aagcagcttg aagagcagtt gctgcatgcc gcgtttcatg
     6001 atttcctgac ggggctaccc aatcgcgccc actttctgga tcgcaccaac cacgcgctgg
     6061 cacgcgcgaa ccggatggaa acagcgattg ccgtgctgtt ccttgacctc gacaacttca
     6121 aagacatcaa cgacagctac gggcacgcgc tgggcgacga actgctgacg cgcgtcgccc
     6181 aggtcctgcg gctccgtgtc cgcgaggggg agctgctgac acgcctcgga ggagatgagt
     6241 tcgcggtgct gctagaagac actggcgaca tcgattccgc cacgcgcgtt gctgagcggc
     6301 tgctggaggc actgcacgag ccgttgttgc tgtccgggcg gaaggtgttt gtctcagcca
     6361 gtgccgggat cgtgaccagc gccccgcgcc acgcgacgcc gggggatctg ttgcgtgacg
     6421 cggatatcgc gctgtatcgg gccaaggacg ccggcaagcg gcagttcgcg atctttgaag
     6481 aggcgatgca cgcggatgtc accgatcggc tcgagctgga atcggatcta cgtcgtgcgc
     6541 tcgtcaacga cgaattcgtt ctggaatatc aaccgaaggt tagtctgacg accagagcga
     6601 tcgtcggtgt cgaagccctg atccgctggg agcatccgac gcgcggacgc atctcgccgg
     6661 caacgttcat tcctatcgca gagcagagcg gtctgatcct cccgatcggt cgctggatcc
     6721 ttcgggaggc gtgtacgcag gccgcaggat ggagtcacgt ctacgacggc gacgggccat
     6781 tcgttgtgag cgtcaatctt tcgggacggc aatttgggga tccacacctg gtcgagcatg
     6841 tctcgagcat cctccacgcc acgggactgg atccggcgta tctcggattg gagatcacgg
     6901 agtcagcagt catggacgat gtcgaactgg cgtgttcccg actacaggag ctcaagaggc
     6961 tcggagttcg cctcggcgtt gatgacttcg gcacaggtta ttcgtcgctg gcctacctca
     7021 agcgcttccc gatcgacgtc ctgaagatcg accgctcatt cgtgaacggc ctggaaagct
     7081 cagcacaaga tcgcgcgatc gtcgcggcga cgatcacgct cgcgcatgcg ctcgggttgc
     7141 gggtcatcgc tgaggggatt gaaacggaag cacaactgac ggagttgcgg cgtcttggtt
     7201 gcgacgcggc tcagggcttc ctcttctctc gccccgtgac gccatcgacg attacgagca
     7261 tgatcgtcca ggctcatcca ttgcccatag cagctacagc aggatcgagc cgtacctcat
     7321 ccgggcggac gccccgccga tccattccac gccggcgccg gccggccgat cacgcgcgac
     7381 gacggcgcag ttgatctcca gctccgcatc gcggtagcgc ccgtcacccg tcgcctgcca
     7441 gacgtgtgcg gcggatatcg gcagtgatgc attccctccg cagcggcgct accggctgac
     7501 gattccggga caactccggg tgaactcggt cacgagccgg acggtgtgct cgaagtcatc
     7561 gagccgcatg atcgcgtagg gcgagtggaa gtagcggacc ggcagcgaga cgacaccagt
     7621 ctggacgcct ccacgactgc gctggatcgc gccggcgttg gtgccgccac cgaatggcac
     7681 gcgatactgc cacggaatca ggtgctcttc ggccagttcg gtgatcgccc tgacgacgcc
     7741 aaccgccgtg atcatcgtgt tgtccataac gacgacggcc ggaccgcggc cgaaccgggt
     7801 cggctggcga gcgggtggaa tcccgggcac gtcagcggcc accgtcgttt cgagcacgag
     7861 cgcgatgtct ggctcgatct gataggcagc cgtcccggcg ccgcgcaacc cgacctcctc
     7921 ctgcacggag aagcaggcga cgacggtgct ctcgagctcc tgccccgcca atgcttcgag
     7981 caccttgacg atcaccgcgc agccggcgcg gtcgtcgatg gcgcgcgcca tcaccatctg
     8041 ctcgttgagg cgctcgaacg ggtacgcgat caccagcggt gagccgacgc ggatgccgag
     8101 gttggcaacc tcctcgacgc tggtcgcacc gacgtcgatg aacaggtctt ccagcttgta
     8161 gggcttgtcg cggtcttcag gccgcaagat gtggggcggt ggcatgccga tgtagccctt
     8221 gaccctggtg ccgtggtcgg tccggatcgt cacggcgtgg gccggcacga tccggggatc
     8281 ccagccgctc gtctgggtga agcgcaggaa tccgccctcc tcgacgaagc tgacaatgaa
     8341 accgatctcg tccatgtggg cgtcgagcat cagcgtcgtg tcaccggttc cgcgccgggt
     8401 ggcgatcaga ttgccgagtg tatcgacgcg catctcatcg acgaacggcg cgatgagccg
     8461 ttgcagcgtc tgacagacct cgtcctcgaa cccggacggt ccaaacacat ccgacagcgt
     8521 cttgagcaat tcgacagtct ccatgctgct ttatcttccg ttcccgcgac tgaagccgca
     8581 tggcgcggca tcctcggccg cgaaatatcc acgcttgaga tcacgctcaa cgaggttggg
     8641 atcgcgctcg gccggatcac cgtatccgcc gccgcccggc gtgcgcacct gtacccagtc
     8701 gccaggctgc aggacgtacc cttccccctt cgagaggtgt tccggacgcg tcaccgcgcc
     8761 actctggctc acctcgatct cgttcatcgc cccagggagc ccgccgaaca ggccatgtgg
     8821 cggagtccgg ccatgttcca tgacgaacga ggcggtcgct tcaccgcgca tgagccgcgc
     8881 gcgatagttg acgccgaagc caccgcgatg ttttccggcg ccggctgatc cctcgcgcag
     8941 ggcgtactcg tcgaagcaga ccgggtagtg ctgctcgagg atctcgatcg gttgtgtctt
     9001 cgagatgcca atcgtcgagc agccgttcgt tagcccatca ccaccccacc agccgccgta
     9061 gccgcccccg ctgaagacgt acatgatgta gcgccgacca gtcgccgggt cgtagccacc
     9121 gagtgagaag ttgccgctgg tgccggccgg cgccgcgaag atccgctcag gcagcacctg
     9181 ggccatcgct ccgaagacgc tctccgcgat gcgctgcgac acctcggcgg cgcaggcggc
     9241 gacgggtcgg ggatactcag catacaggaa ggtcccacgt ggttcgggaa ttcgcagtgg
     9301 c
//
LOCUS       DASUGJ010000608         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1766200, whole genome shotgun sequence.
ACCESSION   DASUGJ010000608 DASUGJ010000000
VERSION     DASUGJ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766200"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..>2146
                     /locus_tag="VFV93_13825"
     CDS             14..>2146
                     /locus_tag="VFV93_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5166476.1"
                     /translation="MGRLPSAARDALAIAAIIGDHLSVELWASVSDLSIEQLAAVIDE
                     AVDVHVLEELPDSAGLRFSHALVRMAIYATMALPRRRAWHLRVAETLLAAPVVSPDEV
                     AYHFQQAGDDRAVEWYVRAGLRARRADAWITAAERFATAASLLEHDDRIRERGWLLFM
                     AGFLWRFSDGKRSIAAHDEAERLAAIARDPILAAYASYARGAVRCMRADVLRGLRDLE
                     TGVAAIDALLHDHLLLSTEEQAMAGFGTLLAQAGVGSEPAPEGRLGSDTPVPESRIPT
                     DGAARVNRQRGNLINHYSHTGRYRDVAAIGEPYLAEMQTGLGANWFRNPQFYSGGHGI
                     GHTYAALGRIEDARRMFALSRAGAEGFNDQAVVEMNLWSEQLMILIPYLTDQPDERRK
                     LIADARHAWERCVGMTITVAGDGAPSEVQCDLLEGRWQQARALAADHLGAPWYTLAQE
                     AIIVLGVLDRLQGDPDGAWQRVRQLLPQGLATEPGSVYFAHAIVAIELAANLALDAGD
                     VATARDWIETHGRWLDWNGASLWRADTQLLWARYHKRIGDPACARRHAGTALTIASDP
                     RQPLRIVAAERLLGQLDANVDSAIDHLARSLALAEACIAPYQQALALLALAELHLHTG
                     RTVAAGTLLERARTICTTLDARPLLARVAELDIRAGAGTTRPDGLTPREVEVLRLIAA
                     GHSNRQIADTLFLSPRTIERHIANIYLKI"
BASE COUNT          344 a          767 c          696 g          339 t
ORIGIN      
        1 catcgatcga cgattgggcc gcttaccctc agccgcgcgc gatgcgttgg cgatcgccgc
       61 catcatcggc gatcacctgt cggtcgagct ctgggcgagc gtcagcgacc tgtccatcga
      121 gcaactggct gcggtcatcg acgaagccgt cgacgtgcac gttctggaag agttgccgga
      181 tagcgcgggc ttgcgcttca gccacgcgct cgtccggatg gcgatctatg cgacgatggc
      241 cctgcctcgc cgccgtgcct ggcacctgcg ggtggccgag acactgctgg ccgcgccggt
      301 cgtcagcccc gacgaggtcg cctaccattt ccagcaggcg ggcgacgatc gcgcggtcga
      361 gtggtatgtg cgggccgggc tccgcgcgcg acgcgccgac gcctggatta cggctgccga
      421 gcgcttcgcg acagccgcgt ctctgctgga acacgacgac cgcatccggg aacgtggctg
      481 gctcctgttc atggccggct ttctgtggcg cttctccgat ggcaaacgct cgatcgccgc
      541 ccatgacgaa gccgaacggc tggcggcgat cgcccgcgat ccgatcctgg ccgcctatgc
      601 cagctacgct cgcggagccg tgcgctgcat gcgggccgat gtcctgcgcg gcctgcgcga
      661 tctcgagacc ggcgtcgcgg ccatcgacgc actgctgcac gatcatctat tgctgtcaac
      721 cgaagagcag gcgatggcgg gcttcgggac actcctggct caggctggag tgggctcaga
      781 acccgcccct gagggccgcc tcggttcgga taccccagtt cccgaatccc gaatcccgac
      841 cgatggtgcc gcgcgcgtca acaggcagcg cgggaacctc atcaaccact acagccacac
      901 gggccgctac cgggatgtcg ccgccatcgg cgagccgtat ctggctgaga tgcagaccgg
      961 gttgggggcg aactggtttc gcaatccgca gttctattcc ggcgggcacg gtatcggcca
     1021 cacctacgcg gcactcggcc gcatcgagga tgcgcgccgc atgttcgctc tctcgcgcgc
     1081 cggagcggaa ggcttcaacg atcaggccgt cgttgagatg aacctgtggt ccgagcagtt
     1141 gatgatcctg atcccctacc tcaccgacca gcccgacgag cgccggaaac tgatcgccga
     1201 cgcccgccac gcctgggaac gctgcgtcgg gatgacgatc actgttgccg gcgacggcgc
     1261 gcccagtgag gtccagtgcg atctgctcga gggccgctgg cagcaggcgc gcgcattggc
     1321 cgccgaccac ctcggtgcgc cgtggtacac gctggctcag gaagcgatca tcgtcctcgg
     1381 cgtcctcgac cgcctgcagg gcgatcctga tggcgcctgg cagcgcgtcc gccaactact
     1441 gccccaagga ctggcaactg agccgggcag tgtctacttc gcgcacgcca tcgtcgcgat
     1501 cgagctggcc gctaacctgg cgctcgacgc tggcgatgtc gcgacggcgc gcgactggat
     1561 cgagacacac ggtcgctggc tcgactggaa cggcgccagc ctctggaggg ccgacaccca
     1621 actcctctgg gcgcgctatc acaagcggat cggggacccg gcctgcgcac gccggcacgc
     1681 cggaaccgcg ctcacaatcg ccagcgaccc gcgtcagccg ctgcggatcg tggccgccga
     1741 gcgcctgctc gggcaactcg acgccaacgt ggacagcgcg atagaccatc ttgcccggtc
     1801 gctggcgttg gccgaagcct gcatcgcgcc gtaccagcag gcgctggcgc tgctggcgct
     1861 ggcggagttg catctgcata ccggccggac cgtcgccgcc gggacgctcc tcgaacgagc
     1921 gcgaacgatc tgcacaacac tcgatgcccg cccgctgctg gcacgcgtgg ccgagcttga
     1981 catcagggcc ggtgccggca caacccgacc cgacggcctg actcctcgcg aggtcgaagt
     2041 ccttcggctg atcgcagccg gccacagcaa ccgccagatc gcggacacgc tcttcctcag
     2101 cccgcgcacg atcgaacgtc acatcgccaa catttacctc aagatc
//
LOCUS       DASUGJ010000609         3986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1904717, whole genome shotgun sequence.
ACCESSION   DASUGJ010000609 DASUGJ010000000
VERSION     DASUGJ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904717"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFV93_13830"
     CDS             <1..180
                     /locus_tag="VFV93_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166477.1"
                     /translation="LGKPARFLDQETQTYTLMFKCMVPGCGESAERTRVRTQIPVPGE
                     RTTRPPWASRDQKGL"
     gene            345..1442
                     /locus_tag="VFV93_13835"
     CDS             345..1442
                     /locus_tag="VFV93_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5166478.1"
                     /translation="MPPLDRSLLDPALVDGARVVVLLVIDGYSAVAHARHGARLARVG
                     HETFQSATLTSVFPSSTAAALTSLQTGVGPGRHGMAGYTLYLPALGRVVNMVRFQPVD
                     GGQFDAQTLDTASFLPVPSIYAILRDAGIESVVVSHRDYANSPLTQVHSGKTHYVGHR
                     TPGEFAALLLREIVRPGRRFVFGYWAGVDMLGHTHGPAADASVAEIDTVLYALRQHVL
                     EPLARLGGDVSIILTADHGLTDIPVADATSMNDINALTGAWRKPATGERRAVGLSLPE
                     PGTRLRLVDALGERAAILDANEAIGAGLYGPGDQHPDLAERIGDTLLLSRGPASFPFV
                     SSRPQQTPSLGAHGSLTPDEMLVPLLAWRFG"
     gene            complement(1542..3317)
                     /locus_tag="VFV93_13840"
     CDS             complement(1542..3317)
                     /locus_tag="VFV93_13840"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX5166479.1"
                     /translation="MSESMARTGVSRLSLAVAMLAFALIVVAGADLPASGTDTCTGWG
                     DCSGWTYLSSASGAIGRLHLAVAGLTGLATIALVALAWLRLRDDSRVIRAAIAALGLI
                     GVQVVAVLLPASDAAASWGATIHFAATALLLALAIYLVALPSWQALRAATTATSRYRT
                     VVGWSVAAIFAVILSGAYLTASGNTTDCGWPACDASGASGVISTDAQGLHRLLVVSAA
                     VLLLTLAVQTVRSQGPSRLRTVAAVALLAIFAVEIVVGGLSATGSDGALISATHFGAA
                     GMSWSLLIVLALATGATQAGTIQTETQRLPLRVAARDYLRVMKPGIIVLLLITTVGAM
                     LVAGPWPSIWLVLATMLGGALASGGASALNCYIDRDIDPVMARTRKRPLPTGRLTPVQ
                     VRSFGLALSVLAVIELWLLVNPLAAMLALAGNVFYVMIYTNWLKRSTPQNIVIGGAAG
                     AFPPLVGWAAVTGTISLPALLLFAIIFYWTPPHFWSLALLKARDYERAGIPMLPVTHG
                     AQYTRKLILLYAVLLVGITMLVVPAGAAGWLYLTLATALGAVFVGMAARMYLEGTSRL
                     AWRLFKFSNYYLTLILAAMVLDKAL"
     gene            complement(3476..3925)
                     /locus_tag="VFV93_13845"
     CDS             complement(3476..3925)
                     /locus_tag="VFV93_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166480.1"
                     /translation="MANSDLIDSLIATYRNLNLTIRPIADAQANQAIGGGKSLRDMVV
                     ELRNNEMVASQQLKLMTLGEGGISGGMPEIAPSAPGLSTRAVLSEFGTAREAILALIR
                     ELPEEEWAAQREGPNGQTTIQAFIEQLAANDRQALSQIEPAIKAGVS"
BASE COUNT          640 a         1381 c         1352 g          613 t
ORIGIN      
        1 ctcggcaagc cggcgcgctt ccttgaccag gagacgcaga cctacacgct gatgttcaag
       61 tgcatggttc ccggctgcgg tgagagcgcc gagcggacgc gtgtccgcac gcagattccc
      121 gtgccgggtg agcgcacgac ccgcccgccc tgggcatcgc gcgaccaaaa gggactctga
      181 cggctcccgc aacgccgccc acgggaaccg acccacggct gaccggctgg tttgccggag
      241 cgacggcctg gtctggcgcg attctgcccg cctacgccgg cggcagcgtc gcgaatgttc
      301 ctgcgtctat cacccaggcg tttggcgttg ggcccggcga tctgttgccg ccactcgacc
      361 gctcgttgct cgacccggcg ctggtcgacg gtgcgcgggt cgtcgtcctg ctggtgatcg
      421 acggctacag cgctgttgcc cacgcccggc atggcgcgcg gctggcgcgg gttggtcacg
      481 agacgttcca gtccgcgacg cttacgtccg tctttccatc atcgacggcc gccgcgctga
      541 ccagccttca gaccggcgtc gggccgggac gacacggcat ggccggctac acgctctacc
      601 tgccggcgct cggccgggtc gtcaacatgg ttcgtttcca gccggtcgac ggcggccagt
      661 tcgacgcgca gacgctcgac accgccagct tcctgccggt tccctcgatc tatgccatcc
      721 tgcgcgacgc cgggatcgag tcggtcgtcg tttcccaccg cgactacgcc aactctccat
      781 tgacgcaggt gcactccggc aagacgcact atgtcggcca ccggacgccg ggcgagttcg
      841 cggcgctgct gctgcgcgag atcgtccggc ccggccggcg cttcgtcttc ggctactggg
      901 ccggcgtcga catgcttggc cacacccacg gcccggctgc cgatgcctcg gtcgccgaga
      961 tcgacaccgt gctctatgcg ctgcggcagc acgttcttga accgctggcg aggctcggcg
     1021 gcgatgtcag tatcatcctg acggccgacc acggcctgac cgacatcccg gtcgccgacg
     1081 cgacctcgat gaacgacatc aacgcgctga ccggcgcctg gcgcaagccg gcaaccggcg
     1141 agcggcgcgc cgtcggcctc tcgttgccgg agccgggtac gcggctgcgg ctcgtggacg
     1201 cgctcggtga gcgggccgcg atcctcgacg ccaacgaggc aatcggggcc ggcctctacg
     1261 gccccggcga ccagcatccg gacctggcag agcgcatcgg cgacacgctg ctgctctcac
     1321 gcggcccggc ctcgttcccc ttcgtctcct cccgcccgca acagaccccc agcctcggcg
     1381 cccacggctc gctgacaccg gacgaaatgc ttgtgccgtt actggcgtgg cggtttgggt
     1441 agttcgggaa tcgggaatcg ggaatcggga atcggggccg ggaaacgtcg cacgctaccc
     1501 aagcggacga tgtctcgcgt ccccgaatcc cgaatcccga gttacagcgc cttatccagc
     1561 accatcgcgg ccaggatcag agtcaggtag tagttggaga acttgaacag gcgccaggcg
     1621 aggcgtgagg tgccttcgag gtacatgcgg gcggccatcc cgacgaagac ggcgccgagc
     1681 gcggtcgcca gcgtcaggta gagccagcct gccgcaccgg ccgggacgac cagcatcgtg
     1741 atgccgacca gcaggacggc gtagagcagg atcagcttgc gcgtgtactg ggcgccgtgg
     1801 gtgaccggca gcatcgggat gccggcccgc tcgtagtcgc gcgccttcag cagcgccaga
     1861 ctccagaagt gtggtggggt ccagtagaag atgatcgcga acagcagtag cgccggcagc
     1921 gagatcgtcc cggtgacggc cgcccagcca accagcggcg ggaaggcgcc ggccgcgccg
     1981 ccgatgacga tgttctgagg ggttgagcgc ttgagccagt tcgtgtagat catgacataa
     2041 aagacgttgc cggccagcgc cagcattgcc gcgaggggat taaccagcag ccacagctcg
     2101 atgacggcca gcacgctgag cgccagcccg aagcttcgga cctgaactgg tgtcagccgg
     2161 ccggtcggca gcgggcgctt gcgagtacgc gccatgaccg ggtcgatgtc gcggtcgatg
     2221 tagcagttca gcgcgctcgc gccgccggat gccagcgcgc cacccagcat cgtcgccagc
     2281 accagccaga tcgacggcca cggcccggca accagcatcg caccaacggt cgtgatcagc
     2341 agcaggacga tgatgcccgg cttcatgact cgcaggtagt cgcgggcggc gacgcggagc
     2401 ggcaggcgtt gtgtctcagt ctggatcgtg ccggcctgag tcgcgccggt tgccagcgcc
     2461 aggacgatca gcagcgacca gctcatgccg gcggcgccga agtgggttgc ggagatcagc
     2521 gcgccgtcgc tgccggtggc gctcagcccg ccgacaacga tctcgaccgc gaagatggcg
     2581 agcagcgcga cggcggcgac ggttcgcaga cgcgacgggc cttgcgagcg cacggtctgg
     2641 acggccagcg tcagcagcag gacggcggca ctgacgacga gcagtcgatg gagtccctgt
     2701 gcgtcggtgg agatgacgcc cgatgctccg gacgcatcac aggccggcca gccgcagtcg
     2761 gtcgtgttac cgctggcggt caggtaggcg ccggacagaa tgacggcgaa gatggccgct
     2821 accgaccagc cgacgacggt ccgatagcgg ctggtcgcgg tcgtcgccgc gcggagtgct
     2881 tgccatgatg gcagcgcgac gaggtagatt gccagcgcca gcagcagcgc tgtcgcagcg
     2941 aagtggatgg tcgcgcccca actggcggct gcatcacttg ccggtagcag gaccgcgacg
     3001 acctggacgc cgatcaaccc gagggccgcg atcgcggcgc gaatcacccg gctgtcgtcc
     3061 cgcaggcgca gccaggccag cgcgacgagc gcgatcgttg ccagacccgt cagcccggcg
     3121 acggcaagat gaaggcggcc aatcgcaccg gacgcgctgg agaggtaggt ccagccggag
     3181 cagtcgcccc agccggtgca ggtatcggtt ccggaggcgg gcaggtcggc gccggcaacg
     3241 acgatcagcg cgaatgccag catcgcgacg gcaagtgaca gccgcgaaac cccagttcgc
     3301 gccatggatt ccgacaccgt gtactccccc tgatggctct gggccgcccc tccggacccc
     3361 gctcgaagtg taaccccgtc gccctgcagc gccaaccagc gccccgccgt ggacggccgg
     3421 gacgtgtagt ggctgatcct gtatcggccc gcgtggggca gcttccgcct tggtactagc
     3481 tcacgccggc cttgatggcg ggttcaatct ggctcagcgc ctggcgatcg ttggcggcca
     3541 actgctcgat gaacgcctgg atcgtcgtct ggccgttcgg gccctcgcgc tgggcggccc
     3601 actcttcttc gggtagctca cgaatcagcg ccagaatcgc ctcgcgggcc gttccgaact
     3661 ccgagaggac cgcgcgcgtg ctcagccccg gcgcgcttgg cgcgatctcc ggcatgccgc
     3721 cactgatccc gccctcgccg agcgtcatca gcttgagctg ctgcgaggct accatctcgt
     3781 tgttgcgcag ctcgacaacc atgtcgcgca gcgatttgcc gccaccaatt gcctggtttg
     3841 cctgcgcgtc cgcgattggc cggatggtca ggttgagatt gcgataggtc gcgatcaggc
     3901 tgtcgatcag gtccgagttc gccacaacac cctcctcata tcggtttccc cggtcgagtc
     3961 ggcagcccac acaggtccgg tgagcc
//
LOCUS       DASUGJ010000610         7299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1071376, whole genome shotgun sequence.
ACCESSION   DASUGJ010000610 DASUGJ010000000
VERSION     DASUGJ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071376"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFV93_13850"
     CDS             <1..297
                     /locus_tag="VFV93_13850"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166481.1"
                     /translation="TDQILVLSGEGDVLQPDDGIVAIGSGAPYAISAARALAQHTKLT
                     PREIVSESMKIAAELCIFTNDQVVIEHAPDAEAKASTDGSPKPARRSRRKAVQA"
     gene            294..1691
                     /gene="hslU"
                     /locus_tag="VFV93_13855"
     CDS             294..1691
                     /gene="hslU"
                     /locus_tag="VFV93_13855"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX5166482.1"
                     /translation="MTALTPKEIVAELDRYIIGQDDAKRMVAVALRNRWRRQQLSEQM
                     RGEVHPSNILMIGPTGVGKTEIARRVARIVNAPFIKVEATKFTEVGYVGRDVESIVRD
                     LVEVAISLQHNERLDAVRDQAAGVAAQRLVDLLVSQLDQKTRTSQNGQVDELVTAPAD
                     AALTPAEKRRITRRRKQLTEMLDREALEDETVEIDVEADFDGYMQPVEIISGVSPEEL
                     HDSVRELIDAFGPRRQQRKVSVREARRILTQQEAARLVDMDAVVESAIKRVEESGVVF
                     IDEIDKTIHNGGDYGPDVSGEGVQRDLLPIVEGSVVMTRYGPVRTDHVLFIAAGSFSS
                     SRPSDLIPELQGRFPLRVELDSLDEEDMYAILTVPENALTRQAAALLASEGVELCFAE
                     DGLREIAAIATDVNARTEDIGARRLLTIVERVLEEISFDAPERKGETITIDATYVDER
                     IGDIASDEDLNNAIL"
     gene            1727..2524
                     /locus_tag="VFV93_13860"
     CDS             1727..2524
                     /locus_tag="VFV93_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5166483.1"
                     /translation="MTNDEVAIADPARMPNPTGDATSTAEGSSYGGHGSRYPDFKLSV
                     IVPVYNEARTIAEILERIRAVEIPKEIIVVDDDSTDGTREQLAKEAERPGTVVHYHPE
                     NRGKGGAVKTGLEHVNGDIVIIQDADLEYDPRDYHVLLRPILEGKTKAVYGSRFLGEH
                     KAMYFWHSMGNKALTLVTNVLFDTTLTDMETCYKVFTADIAHGLDIRQMRWGIDPEIT
                     AKILRSGVRIYEVPISYNGREFWEGKKISWKDAFVVLRTLFRYRFFK"
     gene            2545..3513
                     /locus_tag="VFV93_13865"
     CDS             2545..3513
                     /locus_tag="VFV93_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5166484.1"
                     /translation="MSNSTPSHQIALGVDLGGTNLRSAAVTATGEIVYETHRPTFAAE
                     GVDSVIDRIALAIREAAEHSDAGPDVPVGVAAPGPLDPRSGIVHFAPNLPGWRNVQLR
                     DRLQALTGRTVLLGNDANAAALGEFIFGAGKGTQNLIFVGIGTGVGGGVISEGRLIDG
                     VRGMGAELGHVSVDLGGPRCTCGSAGCIEAFCSAWSITHEAQLLVSSGRGAAILAAAG
                     SDKVGPRAVGSAAAAGDPAAQALLARAGTALGAGLANFINIFNPEMIVLGGGVAEIGD
                     PLLVPVRQAIAAFAMPVMRESVALVPSSLGLKTGIYGSAALVFYAR"
     gene            3915..4868
                     /locus_tag="VFV93_13870"
     CDS             3915..4868
                     /locus_tag="VFV93_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166485.1"
                     /translation="MFAFIVKRVLWFIPTILAVAAVTFVCMNLTPGSPFQPAGANNLR
                     EDIIKQIEKQYGLDKPMPVRFVIYLSKAVRGDFGESYSRRPQKVNDIIRRGFPVSLQL
                     GAMALAMATVGGLALGILSAVNQNGIIDYISATLAILMFSVPSFVLGILLLILFVIWI
                     PDWTGWRYFRVGGWESPRDWILPTIALGAGPLAQLARYTRSSMIDVIRSDYVRTARAK
                     GLAERKVILKHVLKNALIPVVTLIGPLFAALGTGSFFVEQVFRVPGIGVHFVTSMSTK
                     DHPMIMAVVLLYGGFLAVMNLMVDIMYGVIDPRIRLSGREG"
     gene            4872..5789
                     /locus_tag="VFV93_13875"
     CDS             4872..5789
                     /locus_tag="VFV93_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166486.1"
                     /translation="MASTPDNISQAQLLAEAAEAVPTRQSSLWRDAWHRMVRNKLAVL
                     GMIIVAVFVLTAVFADFIAPYGQAEVVHYTLKESSPSWYFPMGIDQNGRDVFSRIVYG
                     ARVSLLVGVVAQLIVLAIGVPIGAIAGYYAGTVDNALMRTVDVFYAVPQLLLVLLLVN
                     MLGPGMKNIFIAIGVTGWVTMARLVRGVMLSNREQEYVKAAKVAGAGSGYIIMRHMLP
                     NSLTPIVVALTFGIPQAIFIEASLSFIGVGIRPPQPSWGQMVGTGQAYIQSVPHLLLF
                     PCLAIALTMLAFTFLGDGLRDALDVRMND"
     gene            5891..6958
                     /locus_tag="VFV93_13880"
     CDS             5891..6958
                     /locus_tag="VFV93_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166487.1"
                     /translation="MARNVLEVNNLKTQFFTRAGVVYAVDGVSFHVGEGETLGIVGES
                     GCGKSVTATSIMRLIPSPPGRIVDGEILLDDGKDRVDILSLDDSEMRNIRGNKIAMIF
                     QDPMTSLNPVLTIGDQITEPLMLHMGMSRREADTRAVELLKRVGIPAAEERMRAYPHQ
                     FSGGMRQRVMIAIALSCNPRVLIADEPTTALDVTIQAQILDLMMHLNREFGTAIIMIT
                     HDLGVVAEICERVNVMYAGQVVETGAAKDLYNNPQHPYTIGLLNSIPRIGENVKEALE
                     PIPGLPPDLLAPPVGCRFRPRCRFRQEKCEESPPLAEVAPGQLARCWFPQGRGATSYM
                     PTATDTTSQASTGAASVPQGT"
     gene            6992..>7299
                     /locus_tag="VFV93_13885"
     CDS             6992..>7299
                     /locus_tag="VFV93_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166488.1"
                     /translation="MATQAKEATIPSPNGANGPADNILEVTGLKKHFHIGGGFLAKNT
                     ALNIRAVDDVTFSVRRGETFGLVGESGCGKTTLGQTIIRLYNPTEGKIVFNGADISTM
                     G"
BASE COUNT         1373 a         2435 c         2262 g         1229 t
ORIGIN      
        1 accgatcaga tcctggtcct ctccggcgag ggcgatgtcc tccagccgga cgacggcatc
       61 gtcgcgatcg gcagcggcgc gccctacgcc atctcggccg cccgcgcgct tgcgcagcac
      121 accaagctga cgccgcgcga gatcgtctcc gagtcgatga agatcgccgc cgaactgtgc
      181 atcttcacca acgaccaggt tgtcatcgag cacgcgcctg acgccgaagc caaagcgtct
      241 acggacggca gccccaagcc agctcgacgc tcgcgccgga aggcggtgca ggcatgacgg
      301 cactgacgcc caaagagatc gttgccgaac tcgaccgtta catcatcggc caggacgacg
      361 ccaagcggat ggtcgcagtc gccctgcgca accgctggcg gcggcaacaa ctctcggaac
      421 agatgcgcgg cgaggtccac ccgagcaaca tcctgatgat cggcccgact ggagtaggca
      481 agaccgagat cgcccgccgc gtcgcacgga tcgtcaacgc cccgttcatc aaggtcgagg
      541 cgaccaagtt caccgaagtc ggctacgtcg gccgcgacgt tgagtcgatc gttcgcgatc
      601 tggtcgaggt ggcgatcagc ctccagcaca acgagcggct cgacgccgtc cgggaccagg
      661 cggccggcgt ggcagcccag cggctggtcg atctgctcgt cagccagctc gaccagaaaa
      721 cgcgtaccag ccagaacggg caggtcgacg aactggtgac cgcgccggcc gacgccgcgt
      781 tgacgccggc cgagaagcgc cggatcaccc gccgccgcaa gcagctgacg gagatgcttg
      841 accgcgaggc actggaggac gagacggtcg agatcgatgt cgaagctgac ttcgacgggt
      901 acatgcagcc agtcgagatc atctcaggcg tctcgcctga ggagttgcac gacagcgtcc
      961 gagaattgat cgacgcgttc ggtccccgcc gccagcagcg caaggtctcg gtccgcgagg
     1021 cgcggcgcat cctcacccag caggaggcgg cgcggctggt cgatatggac gccgtcgttg
     1081 agtccgcgat caagcgcgtc gaagagagcg gcgtcgtctt catcgacgag atcgacaaga
     1141 ccatccacaa cggcggcgac tacggaccgg atgtctccgg cgagggtgtc cagcgcgacc
     1201 tgcttccgat cgtcgaaggc tcggtcgtga tgacgcgcta cggcccggtt cgcaccgatc
     1261 acgtcctctt catcgccgcc ggctcgttct cttcgtcgcg gccatcagac ctgatccccg
     1321 agctgcaggg ccgcttcccg ctgcgggtcg aactcgatag cctcgacgaa gaggatatgt
     1381 atgcgatcct gacggttccg gagaacgcgc tgacccgtca ggcagccgcc ctgctcgcca
     1441 gcgagggggt cgaattgtgc ttcgcggaag atggcctgcg cgagatcgca gcgatcgcga
     1501 ctgacgtgaa tgccagaacc gaggacatcg gcgcccggcg cctgctgacg atcgtcgagc
     1561 gtgtcctcga agagatctcg ttcgacgctc ccgagcgtaa gggcgagacg atcacgatcg
     1621 acgcgaccta tgtagacgaa cgcatcggcg atatcgccag cgacgaggat ctgaacaacg
     1681 ccatcctctg accggacgaa ttgaagggac gcgggggtac gcagacgtga cgaacgacga
     1741 agttgcaatc gctgatccgg cgcgaatgcc gaatcccacc ggcgacgcaa cgtccacagc
     1801 cgaaggatcg tcgtacggcg gacacggcag ccgctacccg gacttcaagc tctccgtcat
     1861 cgttccggtc tacaacgaag cgcgaaccat cgccgagatc ctggagcgca tccgcgccgt
     1921 cgagatcccg aaagagatca tcgtcgtcga tgacgactct accgatggca cccgcgagca
     1981 gctggccaaa gaagccgagc ggcccggcac ggtcgtgcac taccaccccg agaaccgggg
     2041 caagggcggc gccgtcaaga ccggcctgga gcacgtcaac ggcgacatcg tcatcatcca
     2101 ggacgccgac ctcgaatacg atccccgcga ctaccacgtc ctgctgcggc ccatcctcga
     2161 aggcaagacc aaagccgtct acggctcgcg cttcctcggt gagcacaagg cgatgtactt
     2221 ctggcactcg atgggcaaca aggcgctgac actggtgacc aacgtcctct tcgacacgac
     2281 gctgaccgac atggagacct gctacaaggt cttcacggcc gatatcgccc acgggctcga
     2341 catccgacag atgcgttggg gcatcgaccc ggagatcacc gccaagatcc tgcgtagcgg
     2401 cgttcgcatc tacgaagtgc cgatctcgta caatggccgc gagttctggg aaggcaagaa
     2461 gatctcctgg aaggacgcct ttgtcgtcct gcggacgctg ttccgctatc gcttcttcaa
     2521 gtagtcgatc gggtccagac gccgatgagc aactccacgc ccagccatca gatcgccctg
     2581 ggggtcgacc tcggcgggac caacctgcgc tcggcggccg tgactgcgac cggcgagatt
     2641 gtctacgaga cacaccggcc gacgtttgcg gccgagggcg tcgattcggt gatcgaccgg
     2701 atcgcgctcg ccattcgcga agcggccgag cactcggacg ccggccccga tgtccccgtc
     2761 ggcgtcgccg cgccggggcc gctcgatcca cgcagcggca tagtccactt cgccccgaac
     2821 ctgcctggct ggcgcaacgt ccagctccgc gaccggctcc aggcactgac cggccggacc
     2881 gtcctgcttg gcaatgacgc caacgcggcc gcgctgggcg agttcatctt cggcgccggc
     2941 aaaggtaccc agaatctgat cttcgtcggt attggcaccg gcgtcggcgg cggtgtcatc
     3001 tcggaagggc gactgatcga cggtgtgcgc ggcatgggcg ccgaactggg ccacgtcagc
     3061 gtcgacctgg gcggcccgcg ctgcacctgc ggttcggcag gctgcatcga agcgttctgt
     3121 tcggcatggt cgatcacgca cgaggcgcaa ctgctggtct cgtccgggcg cggcgccgcg
     3181 atcctggcag ccgccggcag cgacaaggtc ggcccgcgtg cggttggcag cgctgccgcc
     3241 gcaggtgatc ccgcagcaca ggcgctgctg gcgagggccg gaacggcgct cggtgccggt
     3301 ctggcgaact tcatcaacat cttcaatccg gagatgatcg tactcggcgg tggcgtcgct
     3361 gagatcggag acccgctgct ggtacccgtc cgccaggcca tcgcggcgtt cgcaatgccc
     3421 gtcatgcggg agagcgtcgc gctcgtcccc tcctcactcg gcctcaagac cggcatctac
     3481 ggctcggccg cgctcgtgtt ctatgcgcga taggtgacag gtgacacccg tcacctgtca
     3541 tctatcacct cgctctccgc ttgacaccca acaaagcgca tgtgtatagt gcgcgccgag
     3601 cgaatcgcac taccaatcca ctccagtcat cggttgcgtc acctgctggg aggttgaggc
     3661 aaccagtcgc gcagtagcat cggttacggt accgtcgaca gacgccggcc aacgcggccg
     3721 ttctgctccg tggagtggaa ttgcggcgta ttcggctatc cgtcagcggc acgccggcca
     3781 acgtggcgac ccgggtcgcc agccgagaaa ccgtcgatca gttgggcgcc agccgcccga
     3841 ggtcggttcg gatcacgcgt cgccacctgg tgatgcaccg gccggcggga tgtgggagaa
     3901 aggacgaacc tggcatgttt gccttcatcg tcaagcgggt attgtggttc attcccacaa
     3961 tcctcgccgt cgccgccgtg acgtttgtct gcatgaatct gacgccgggt tcgccgttcc
     4021 agccggcagg cgccaacaac ctccgcgaag acatcatcaa gcagatcgag aagcagtacg
     4081 ggctggataa gccgatgccg gtccgcttcg tgatttatct ctcgaaggcc gtccgcggcg
     4141 acttcggcga gtcctactca cgccgaccgc agaaggtgaa cgacatcatc cgccgcggct
     4201 tcccagtctc gctccagctc ggcgcgatgg cactggcgat ggcgaccgtc ggcgggctgg
     4261 cgctgggcat cctctcggcc gtcaaccaga acggcatcat cgactacatc agcgcgacgc
     4321 tggccatcct catgttctcg gtacccagct tcgtgctggg cattttgctg ctgatcctgt
     4381 tcgtcatctg gatacctgac tggaccggct ggcgctactt cagagtcgga ggctgggaaa
     4441 gtcccagaga ctggatcttg cccacgattg cgctcggcgc tggtccactg gcccagctcg
     4501 cgcgctacac ccgctccagc atgatcgacg tcatccgttc ggactacgtc cggacggcgc
     4561 gggccaaggg gctcgccgag cgcaaagtca tcctcaagca cgtcctcaag aacgccctga
     4621 ttccggtggt tacactgatc ggcccgctct tcgcggcgct cggcaccggc tcgttcttcg
     4681 tcgagcaggt cttccgcgtg cctggcatcg gcgtccactt cgtgacgagc atgtccacca
     4741 aagaccaccc gatgatcatg gcggtggtcc tgctctacgg cggattcctg gccgtcatga
     4801 acctgatggt ggatatcatg tacggcgtga ttgatccacg catccgcctc agtggcagag
     4861 agggctaacc gatggcttcc acacctgaca acatcagcca ggcccaactg ctcgctgaag
     4921 ctgccgaagc ggtgccaacc aggcaatcga gtctctggcg cgatgcctgg caccggatgg
     4981 tgcgcaacaa gctggccgtc ctcgggatga tcatcgtcgc cgttttcgtc ctgacagccg
     5041 tcttcgccga tttcatcgcg ccctacggcc aggcagaagt cgtccactac acgctcaagg
     5101 aatcgtcgcc gtcgtggtac ttcccgatgg gtatcgacca gaacggtcgc gatgtcttca
     5161 gccgcatcgt ctacggcgca cgcgtctcgc tgctggtcgg cgtcgtcgcg cagctcatcg
     5221 tcctcgcgat tggcgttccg atcggcgcga tcgccggcta ctacgcgggc accgtcgaca
     5281 acgcgctgat gcggacggtt gacgtcttct acgccgtccc acaactgctg ctcgtcctgc
     5341 tgctcgtcaa catgctcggg cccggcatga aaaacatctt catcgccatt ggtgtaaccg
     5401 gctgggtgac gatggcacgc cttgtcagag gcgtcatgtt atcgaaccgc gaacaggaat
     5461 atgtcaaggc ggccaaggta gccggcgccg gcagcggcta catcatcatg cgccacatgc
     5521 tgccgaacag cctgacgccg atcgtcgtcg cgctgacgtt cggcatcccg caggcgatct
     5581 tcatcgaggc gtcactctcg ttcatcggcg tcggcatccg cccgccacaa ccaagctggg
     5641 ggcagatggt cggcactggc caggcctata ttcagtcggt cccgcacctg ctgcttttcc
     5701 cctgcctggc aatcgccctg acgatgctcg ccttcacatt ccttggcgac ggcctgcggg
     5761 acgcactcga tgtgcgcatg aatgactaga cgaccgatga caacgatcaa tgatggaatc
     5821 agcgaactga ggggtacagg tccgatgaac acggtacgac agcccgaaga cgtgaggagc
     5881 ggggacgacc gtggctagaa acgtgctcga ggtcaacaac ctcaagacgc agttcttcac
     5941 gcgcgccggg gtcgtctacg ctgttgatgg cgtctcgttc cacgtcggcg agggcgaaac
     6001 actcgggatc gtcggcgaat ccggctgcgg caagagcgtg accgcaacgt cgatcatgcg
     6061 tctcatcccc agtccacccg ggcgtatcgt cgatggtgag atcctgctcg acgacgggaa
     6121 ggatcgcgtt gacatcctct cgctcgacga cagcgagatg cgcaacatcc gcggcaacaa
     6181 gatcgcgatg atcttccagg acccgatgac ctcgctcaac ccagtcctga caatcggcga
     6241 ccagatcacc gagccgctga tgctgcacat gggtatgtcc cgccgtgagg cggatacccg
     6301 cgctgtcgaa ctgctcaaac gcgtcggcat accagcggcc gaggagcgga tgcgggccta
     6361 cccgcaccag ttctcgggcg gcatgcgcca gcgtgtgatg atcgcgattg ccctctcctg
     6421 caatccgcgc gtcctgatcg ctgacgagcc gacgacggcg ctggatgtca cgatccaggc
     6481 gcagatcctc gacctgatga tgcacctcaa ccgcgagttc gggacggcca tcatcatgat
     6541 cacccacgat ctgggtgtcg tggctgagat ctgcgagcgg gtcaacgtga tgtacgccgg
     6601 ccaggtcgtc gaaaccgggg ccgcgaagga cctctacaat aacccgcaac acccctacac
     6661 aatcggcctg ctgaactcga tcccgcgcat cggcgagaac gtcaaggaag cactggagcc
     6721 gattcccggc ctgccgccgg acctgctggc gccgccggtc ggctgccgct tccgcccgcg
     6781 ctgccgcttc cgccaggaga agtgcgagga atcgccgccg ctggcagagg tcgcgccggg
     6841 acagctggcg cgctgctggt tcccgcaggg gcgtggcgcg acgtcataca tgccgacggc
     6901 aaccgacacg acgtcccaag cgagcaccgg agcggcctcc gtgccgcaag gaacgtagcg
     6961 ggccggatta ctgactggaa cggtggagac ggtggcaacc caagccaaag aagcaaccat
     7021 accctcgcca aacggtgcaa acggcccggc cgacaacatt ctcgaggtta cgggactgaa
     7081 gaagcacttc cacatcggag ggggcttcct cgcaaagaac actgcgctga acattcgcgc
     7141 cgtcgatgat gtgaccttct ccgtccggcg cggggagacg ttcgggctgg tcggcgagtc
     7201 cggctgcggc aagacgaccc tcggccagac gatcatccgt ctctacaatc cgaccgaggg
     7261 caagatcgtc ttcaacggcg ccgacatctc aacgatggg
//
LOCUS       DASUGJ010000611         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1487875, whole genome shotgun sequence.
ACCESSION   DASUGJ010000611 DASUGJ010000000
VERSION     DASUGJ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487875"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFV93_13890"
     CDS             complement(<1..740)
                     /locus_tag="VFV93_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166489.1"
                     /translation="MTATIRIFTIEARRSVALWFVPLMVAVAGWYANARLSPVGVAVW
                     RHSSVSVGETLFVLAPLLGGVAAWAGGRDRRRGVGDLLATMPRPLAHRTLADWGGVVA
                     WVVLAYAIVGTFVAVVTMRDDPWGSPIAAPVVIGLLTIVAASAIGYLTGALIPSRFVP
                     PLVPIGLFLTIGLLDAGWLRGNRISYLSPATLLDRQLLFMNSDSFFFRPPSLHLLPMV
                     LWLSGLTGLALTGVVLTRRRSAAGWSCLS"
     gene            complement(737..1486)
                     /locus_tag="VFV93_13895"
     CDS             complement(737..1486)
                     /locus_tag="VFV93_13895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166490.1"
                     /translation="MAVNVDVQGLTQRFGRTTVLDGLDLRVGTGVFGLLGPNGAGKTT
                     LLRTLATIIRPKAGSLRLLGHDPGSDHERRLLRRRLGYLPQQLGYYPNFTVAEFVEYF
                     ALLKEMPNDEVRPAVARAIARVGLEERGRSKLKTLSGGMLRRVGIAQAIVNDPDLLLL
                     DEPTAGLDPEQRVVFRTLLREIGTDATVIVSTHLVEDVAAACTEVALLSSGRIAFRGT
                     PEELTLLGEGYTVGDSPLERGYSRALAGARG"
     gene            complement(1477..2253)
                     /locus_tag="VFV93_13900"
     CDS             complement(1477..2253)
                     /locus_tag="VFV93_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166491.1"
                     /translation="MTIDNLHPDDPAHIGGCAVCRAAVAGGLDVDLDRVWAAVAGEVI
                     ARPVGRVERFAGRLLHSPGLARAVVETPSLVLSWLLASAVVLAIGVIVTAASGGGTPW
                     VALLAPALAGAGVAYAYGPGIDPAFELAQTMAVPDQLVLLARVLAVFGVNAVFGLAAS
                     LVAAPVAGLTLGWLLPMTTVAALGLAAATLARSANVGVAAGLAGWGLIVLGSAVDTRD
                     LATAVERGWLAPFYLIGTIVFVGLALHATSGARDRGLGWQ"
     gene            complement(2250..2762)
                     /locus_tag="VFV93_13905"
     CDS             complement(2250..2762)
                     /locus_tag="VFV93_13905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5166492.1"
                     /translation="MNDDELIAAVGAGDQMALKLLFERHAPWIAGRLRRTLPVDAVED
                     VVQETFIAVWRGAGSFAGGSEVGGWLWGIARRQAVNWLRKHGRIAPDVWPVEASDPAN
                     AAITRVDLQRAAATLGPEERELLRLLYVEDRPVAEVARELDIPAGTVKSRANRLRRTL
                     RAALGMGDGA"
     gene            2963..>3548
                     /locus_tag="VFV93_13910"
     CDS             2963..>3548
                     /locus_tag="VFV93_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX5166493.1"
                     /translation="MSLRLVIVGLPQSGKTTVFNALTRSEAVTGAFSSGEAEPNLATV
                     KVPDPRLDALTPLFNPKRVVPADVQYLDVAGVAKGLAESGMGGQLLGHLAQADALVLV
                     VRAFEDSNVPHPEESVDALRDLETLFLEFTFSDLAIIEKRLVRIQATIPKLRGNEKEQ
                     YEREREALVRCKAALEDGTPIREVELDEEEERLLR"
BASE COUNT          645 a         1319 c         1107 g          477 t
ORIGIN      
        1 gacaaacagg accagcccgc agcactgcgc ctccgcgtca gcaccacgcc cgtcagcgcc
       61 aatcccgtca gtccggacag ccagagcacc atcggtagca ggtgcaacga tggcggcctg
      121 aagaagaagg agtccgagtt catgaacagc agctggcggt cgagcaacgt cgcgggtgac
      181 aggtacgaga tgcgatttcc gcggagccag ccggcatcga gcaagccgat cgtgaggaag
      241 agtccaattg gaacgagcgg cgggacgaag cggctgggta tcagcgcgcc tgtcaggtaa
      301 ccgatcgcag acgcggccac gatcgtcagc agcccgatga cgaccggcgc agcgatgggc
      361 gatccccagg gatcgtcgcg catcgtcacg acggcgacga acgttccgac gatggcgtag
      421 gcaagaacaa cccaggcgac caccccgccc cagtcggcca gcgtgcgatg cgccaacggt
      481 cgcggcatcg tcgccagcag atcgccgacc ccgcgccgcc gatcgcgccc gcctgcccag
      541 gcagccacgc cacccagtaa cggagcaagg acgaacagcg tctcgccgac cgagacgctg
      601 ctgtgtcgcc agacggcgac accgaccggc gacaaccggg cgttcgcgta ccagccggca
      661 accgctacca tcaacgggac gaaccagagc gcgacgctgc gccgcgcctc gatcgtgaag
      721 atcctgattg tcgccgtcat ccgcgcgctc cggccagcgc acggctgtag ccccgctcca
      781 agggcgagtc gccgaccgtg tagccctcgc ccagcagtgt caactcctcc ggtgtgcccc
      841 ggaaggcgat ccggccgctg ctcagcaagg cgacctcggt gcaggccgcc gcgacatcct
      901 cgaccaagtg ggtactgacg atgacggtcg cgtctgtgcc gatctcgcgc agcagcgtcc
      961 gaaagacgac gcgttgttcc gggtccagcc cggccgtcgg ctcgtcgagc agcaagaggt
     1021 ccgggtcgtt gacgatcgcc tgggcgatgc caacccgccg caacatcccg cctgagagcg
     1081 tcttgagctt cgaccgtccg cgctcctcca gcccgacccg cgcgatggca cgggcgacgg
     1141 ccggccggac ctcgtcgttc ggcatctcct tcagcaacgc gaagtactcg acgaactccg
     1201 cgacggtgaa gttggggtag tagccgagct gctgcggcag gtagccgagc cggcgccgca
     1261 acagccgccg ctcatggtcg ctgcccggat cgtgacccag cagccgcagc gaccccgcct
     1321 tcggccggat gatcgtcgcc agcgtccgca gcagcgtcgt cttgccggcc ccgttcggac
     1381 ccagcagccc gaagacgccc gttccgaccc gcagatccag cccatcgagg acggtcgtcc
     1441 gtccgaagcg ctgcgtcagt ccctgaacat cgacgttcac tgccatccca gcccccgatc
     1501 ccgcgcacca ctggtcgcgt ggagcgccag cccaacgaag acgatcgttc caatcagata
     1561 gaagggcgcc agccaccccc gctcgaccgc agtcgcaaga tcgcgcgtgt cgacggcgct
     1621 gccgagcacg atcagccccc agccggccag cccggccgcg acgccgacgt tggccgagcg
     1681 tgccagcgtc gccgccgcca gcccgagcgc ggccaccgtc gtcatcggca gcagccagcc
     1741 caacgtcagc ccagccaccg gcgcggccac cagcgacgcg gccagcccga aaacggcgtt
     1801 gacgccaaag acggccagca cccgcgccag cagcaccagc tgatccggca cggccatcgt
     1861 ctgcgccagc tcgaaggccg gatcgatgcc cggcccgtag gcatacgcca cacccgcccc
     1921 ggccagggcc ggcgccagca gcgctaccca cggcgtccca ccgcccgagg ccgccgtcac
     1981 gatgacaccg atcgccagga cgacggcgct ggccaggagc cacgacagca ccagcgacgg
     2041 cgtctcaacc accgcccgcg ccagtcccgg cgagtgcagc agccggccgg cgaagcgctc
     2101 gacccgccca accggtctgg cgattacctc accggccaca gcggcccaga cccggtccag
     2161 atcgacatcc aacccgcccg caaccgccgc gcggcaaaca gcgcaaccgc cgatatgggc
     2221 gggatcgtcg ggatgcaggt tgtcgatcgt catgctccgt ctcccattcc cagcgcggcc
     2281 cgcagcgttc tccgcagccg gttggcgcgg ctcttgaccg tcccggccgg aatgtcgagc
     2341 tcgcgcgcga cctcggcgac cggccggtcc tcgacgtaga gcagtcgcaa taactcacgc
     2401 tcctccggcc ccaacgtcgc cgccgcccgt tgcagatcaa cccgtgtgat ggccgcgttc
     2461 gcggggtcgc tcgcttccac cggccagaca tccggcgcga tccgcccgtg cttccgtagc
     2521 cagttcaccg cctgccgccg cgcgatcccc cacagccagc cgccaacctc gctgccgcct
     2581 gcgaacgacc ccgccccacg ccagaccgcg atgaacgtct cctgcaccac atcctcgaca
     2641 gcatcgaccg gcagcgtccg ccgcaatcgc ccagcgatcc acggcgcatg ccgctcgaac
     2701 aacagcttca gggccatctg atcgccagcg ccgaccgccg cgatcaactc gtcatcattc
     2761 atcgctacca gtttgtcgca ttgggggcgc atttggttca tcgtgcgtaa atcgtgattc
     2821 tggattcggg gctcgggaaa catcatttgc gaaggtagcg tgcaacgttt accaaccccg
     2881 agtcccgagt cccgaatccc accccatatg attcaattca ctgttttgca cgttcgggga
     2941 cactggggag ggtacgcacg caatgtcgct tcgtctggtc atcgttggct tgccgcagag
     3001 cgggaagacg accgtcttca acgccctgac gcgctcggag gcggtgacgg gtgcgttcag
     3061 ctccggcgag gccgagccga acctggcgac cgtcaaggtg ccggacccgc ggctggatgc
     3121 cctgacgccg ctcttcaacc ccaagcgggt ggttcctgcc gacgtccagt acctcgatgt
     3181 ggccggcgtc gcgaaagggc tggccgagtc ggggatgggc gggcaactgc tcggccacct
     3241 cgcccaggcc gacgcgctgg tgctggttgt gcgcgccttc gaggacagca acgtcccaca
     3301 cccggaggag agcgtcgacg cgctgcgcga tctggagacg ctgttcctgg agttcacatt
     3361 ctccgacctg gccatcatcg agaagcgcct cgtcaggatc caggcgacga tcccgaagct
     3421 gcgcggcaat gagaaggaac agtacgagcg cgaacgcgag gcgctggtgc gctgcaaggc
     3481 ggcactcgaa gacggcacgc cgattcgcga ggttgagctt gacgaagagg aagagcggct
     3541 gctgcgcg
//
LOCUS       DASUGJ010000612        12888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_239509, whole genome shotgun sequence.
ACCESSION   DASUGJ010000612 DASUGJ010000000
VERSION     DASUGJ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12888
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239509"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="VFV93_13915"
     CDS             <1..1977
                     /locus_tag="VFV93_13915"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEX5166494.1"
                     /translation="LPLWGTYANPRAFYGSALLLDAGTTYEIRVSLNDPDGVNGNFTV
                     TGSIATRGEDIAGASQLKPTQFVSSQGNDANNGTSPSSAWKTLEKAFNSAPAGAVVSV
                     APGSYAPPTTNRTLPITLVAENPAVDDGQNIINAGKHSVIEPQTITERNSGAWTNVKL
                     KGPATGQTYSVWKWAGSPVANATVLAWSNSRTDEPQRVGYWNRRDGTFDGYSMETPEG
                     WSEVLYRNETYNFGFASFGNDVYLRLPGDKNPNDVVMTLHDTWKSNDTGRLVLSGSNV
                     RLSGFELRLVDAMLRSPSVNGVIDHNLFYMGTVYYKGDKGTPSSYPTNQVVQYNRMID
                     TSIRPRDGGTGVIPWLFVKQNLKLNGKETNWRRTGEAAEQHAIEGRGGANQLVVRHNT
                     IEGYFNGVGANSTDFDRYSKAGADIYDNAFSHIADDTIEPEGDTINWRVWSNRIEYAS
                     VAMSTGPVDFGPIYFFRNEIWMLGNQGVGPDRSGDSGVGSYAFKYSGSSNPLARIYVV
                     HNTFWTDKAGTNGGLQAAGGGSDTERFYLRNNIFRVTRYAFDSPGSGGGWNEDYDFFF
                     TSDTGRGINYGTRNYKTVSEYRKVSGQGAHTNLSDKTDRFKAEPGLVDPARGNLDLAN
                     GSAQIDAGVVVPNISDQAGVDYNGAAPDLGANER"
     gene            complement(2092..3630)
                     /locus_tag="VFV93_13920"
     CDS             complement(2092..3630)
                     /locus_tag="VFV93_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX5166495.1"
                     /translation="MSARPGQLPTVEAGTLELGHPAHDAEITIRKRAAGGAVWIFVSF
                     ALSKVFGLASNVVLARLLTPEHFGLVAFAMVVIGAFTLLQDLGVPDALIYNQRTTTEL
                     LQTALAINLGAAVVLFCISALLAPLLARLGGDATIAPIVIVLAIGLIFSAAGSVQNAL
                     LSKQLAFRRRTLPDIVSLATESVVAIGLALAGFGVWSLVIGFLTLNLTKTLMLWYLSD
                     AHPLPRYHASLAGQLVGYGKHVSLNAIVGFAANNVDYLIVGYVLGPRALGIYTLAFMV
                     ANIPTTAISQVLRTAMFPVYASLRQDRQRMVTLFEDVFVVVWTLSIGTGLVIFICAPL
                     YMSLLLGDKWSTIGDPLRILTIFGVLRSIGAVFPPVYKALGRPDMDWKFTLARLLVSA
                     PLMMLVAGAGVNAVSGIHAIVAAILVPLNAVFFARVAEVGLARIMRLIVGQLAGVAGV
                     ALLIALGRTTPSLRDAGSHPFELLLVVILASAVYLGAVVSLNRQLLARLLPALRATRP
                     AGMR"
     gene            complement(3647..4564)
                     /locus_tag="VFV93_13925"
     CDS             complement(3647..4564)
                     /locus_tag="VFV93_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5166496.1"
                     /translation="MSAATPSVKVIYISGATRSGSTILTQMLGELDDSFAPGELRLIW
                     ERSFLRNDLCGCGEPFRSCPTWQQVVNDAFDGPDGVDPCAMIAHQPRLRHLPLMLMGG
                     DSARVQRAFGPYLTMLDRFYRSIGEVTQSRVIVDSSKSPMYGFLLNLLPSVDLYVVHL
                     VRDPRGVQYSRMRRRQRGALNGRRAGIAVSALMWNLRNGVQEILTRRSGQPYLRIRYE
                     DLIASPLQTIEAIKTFITEPTIGLPEIGDGEIRLRPNHSIAGSDTRADIGVVRLRVDA
                     AWRTGLERRRRVLVTALTYPFLRRYGYSR"
     gene            complement(4627..5226)
                     /gene="cysC"
                     /locus_tag="VFV93_13930"
     CDS             complement(4627..5226)
                     /gene="cysC"
                     /locus_tag="VFV93_13930"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX5166497.1"
                     /translation="MSNRSDEREASPMQQRGVTVWFTGLSGAGKTTICQRVAQALRDR
                     ALNVEVLDGDIVRENLTKGLGFSKADRDENIRRIGFVSQLLTRNGVIVLVAAISPYRA
                     ARDEVRTRIGDFVEVYVNAPLEICEARDVKGLYEKARAGQIKQFTGIDDPYEPPLLPD
                     VECHTDRETLEESVTKVLATLVARNFLPANGLVPQPALL"
     gene            complement(5223..6440)
                     /gene="sat"
                     /locus_tag="VFV93_13935"
     CDS             complement(5223..6440)
                     /gene="sat"
                     /locus_tag="VFV93_13935"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX5166498.1"
                     /translation="MLDQQTAPRTDVTRRELIAPHGGTLVNQIAPTEVQPALRASAAA
                     LPTIELDARALADLECIATGIYSPYTGFAGKADYDSIVDDMRLANGLPWTIPVTLPVG
                     ADQARRIGLDRQVALTHGGRPLAIMTVTDIYQRDKPREAQNVYGTTESAHPGVAAVYA
                     AGDICLGGSILLIDRIPHDDFFECRLTPVETRAEFARRGWRTVVAFQTRNPIHRAHEY
                     LQKVALEIVDGLFVNPLVGTTKGDDVPAAVRMRTYEVILGTYYPAERTLLSVFPAAMR
                     YAGPREAIMHAISRKNYGCTHFIVGRDHAGVGNYYGTYASQEIFDRFAPDEIGITPLK
                     FEHSHFCLKCGQLVTSKTCPHGQEHWVHLSGTKVRELLAKGEMPPPEFSRPEVAAILI
                     EAYAQSTGVAADR"
     gene            complement(6602..7525)
                     /locus_tag="VFV93_13940"
     CDS             complement(6602..7525)
                     /locus_tag="VFV93_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5166499.1"
                     /translation="MAVETPVKVLYIAGFERSGSTLLTKVAGELEHTFAAAELRGIWH
                     RSFQTNQECGCGQGFRECDLWQEIIADAYGGYDNVDWQRMIDLRPKRRHTPLLLMPGS
                     QPVIARMFREYIATLDQLYRSIATVTDSRVIVDSSKTPLYAAVLGLAPSVDLRVLLLI
                     RNPHGVQSSNLRRARDGDRMLGTLDTYSVSRSLLEWDALHATQELLGLRQRKRYMRMR
                     YEDFVADPVASIEQIKQFLDEPDIGIPRFKDGTVYLSPNHTVCGNRNRIETGAVPLRL
                     DERWRTDLDADTRTLVRRLTLPLLHRYGYAG"
     gene            complement(7617..8375)
                     /locus_tag="VFV93_13945"
     CDS             complement(7617..8375)
                     /locus_tag="VFV93_13945"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX5166500.1"
                     /translation="MPACFEHDAAARIATEAGQVLLALRERAARQEIAPQVLRDKADH
                     RSHDMLMRALTASFPNDPILSEEANDDGARLDSRRVWIIDPLDGTREYGEPGRTDWAV
                     HVALVIDEQPLAGAVALPARGMTLSTAEPPMLPDRRTGPARIVVSRTRPPAVALDLEE
                     RLGADLLEMGSAGAKAMAVVLGEADIYVHSGGQHEWDSAAPVAVAAAAGLHVSRIDGT
                     PLRYNQPDSWLPDLLICRSELRDQVLDALGSALT"
     gene            complement(8393..9385)
                     /locus_tag="VFV93_13950"
     CDS             complement(8393..9385)
                     /locus_tag="VFV93_13950"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5166501.1"
                     /translation="MKDDVPLVSYRVSRTAALVHATRPFVNIHRPGSLPNVFVASLPR
                     SGSTWLMELIWSQPGFKPCDEPTDLRNPYVRKHLGITEWGQLYSDDAGERLFHYFDRL
                     CNGQLGFLNPRPFRRYYRPATSRIVFKVIHGCEGRLNWLRDTFDGRIVYLVRHPVAVS
                     LSREVLPTLPAMLDGPYRSHFTREQLEFAADIVRSGSKLERGVLSWCLQTSVPLHDAT
                     DDWVIASYEQLILDPATVVRHLAAKLELPNPQRMLDAIATPSQSSRKSDSATRKLLNA
                     DAPTRAQRLVEKWRDQVSDADERRAMEILDCFDIDFYRAGDPLPDRRYWLTDTD"
     gene            complement(9440..10615)
                     /locus_tag="VFV93_13955"
     CDS             complement(9440..10615)
                     /locus_tag="VFV93_13955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5166502.1"
                     /translation="MYTILYPVNKLIIGGAEQQLLELVRGLDKEQFQPIVAPLYPGGG
                     LEDEFRAVPGIEVIDLNRRSKYDLRPLWTVTRLLRSRRVDIVQPFLSPATFFGLLPAL
                     TRNATTTIVTERCGVRQSRGVGYKTYRTVEDVFSRLADAIVPNSAAGAEMLVRRGLPR
                     EKIRVIYNGINLDRLTVDASAAAAHRARIAPDGQACVAGMLASLTPAKGHSTAIRALA
                     TLREHQMPVKLAIVGDGPLRSELEALVVELGLTDAVTFFGYQRNVSDFLAAFDVLISS
                     SRDNEGCSNSILEAMALGVPVIATDVGGNPELVKPGETGQLVIPDDPTGLASALVRII
                     EEPVEAREMASRARTLIDARFSLAQMIGSYEELYTQLLRRSLAVVDADGRLVTMKAS"
     gene            complement(10615..12093)
                     /locus_tag="VFV93_13960"
     CDS             complement(10615..12093)
                     /locus_tag="VFV93_13960"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5166503.1"
                     /translation="MNDTIAGRPSRGIWAASGRAAEFSFRRLLLPILAATALLSFVFA
                     ESGNLTAAWLTAFIFATFILAMYGPAWVLAPILINEMTLYGFTNPTFGIAQRFVIAAI
                     ALAIALPAIFRARLSGDRYARRVVLPAIGFLAIVTLMNIQHSDDGYVYQYLRYQLVQF
                     AILVIAACVLYTIPDMKRIALVVAVVTTGVALIVIWQHYAPATALYGPRDDTVKGRAV
                     GLTKGPVPIATQLIFVLAPMLGVLIAKPLRLRRGYLLLAGSTVLTLAALNFTYTRSAL
                     FALAPALVVMGLLFGGQRRTLMISAVIVVIVLFFGLENSGVFGSRYYEGSDEDRSAAS
                     HQALQDVSLAIALDHPLDGIGHEAFTDVSLDYIDDIAVQISADGGASALGQQEPHNDY
                     WNIWLSWGIVALALYIALIVATFRNLRIAARHRDRFVRGLAIGCIGGLVAYCVNSAYH
                     NSLDSSMSLWLYAGISIALARLARADAASMRRQRQQTGLGAP"
     gene            complement(12133..>12888)
                     /locus_tag="VFV93_13965"
     CDS             complement(12133..>12888)
                     /locus_tag="VFV93_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166504.1"
                     /translation="QVNQQIEFTRQQLATSALRQREIDLTDELRDRQSALLDLQTNYQ
                     EELGRQAESDAIGDRSSFSEDALAQQAEVRTQLLRAISQQISDTQANIESIQGELSTV
                     QADIAKLPADEDGSLSAAFSNAYSLQLSTLTEQFVAGQVTALTQGAPVMLYGDASTPF
                     ATMGMKKLGVIGIAGGLAVAAALGLGLDLLRSRRAERARKHGRYPVTSTATPDFERLV
                     ALVDELSVRNDHGGNRSVRSGSHPAGNPTPAGD"
BASE COUNT         2280 a         4066 c         4338 g         2204 t
ORIGIN      
        1 ctgccgctct ggggcaccta cgcgaacccg cgtgccttct atggttcggc gctcctgctg
       61 gacgccggca cgacgtatga gatccgcgtt tcgctcaatg atccagatgg tgtcaacggc
      121 aacttcaccg tgaccggcag catcgcgacc cgtggcgagg acatcgctgg agcgagccag
      181 ctcaagccga cccagtttgt cagcagccag ggcaacgacg ccaacaacgg gaccagcccg
      241 agcagcgcct ggaagacgct tgagaaggcg ttcaattcgg cgcccgccgg ggcggtcgtc
      301 agcgtcgcgc cagggtcgta tgcaccgccg accacaaacc ggacgctgcc gatcacgctc
      361 gtggccgaga atccggccgt ggatgatggc cagaacatca tcaacgccgg caagcacagt
      421 gtcatcgagc cgcagacgat caccgaacgc aacagcggcg cgtggacaaa cgtcaagctg
      481 aagggacctg ccaccggtca gacctactcg gtctggaagt gggccggtag ccccgtcgcc
      541 aacgcaactg tcctggcctg gtcgaactca cggaccgacg agccgcagcg cgttgggtac
      601 tggaaccgcc gtgacggtac gttcgatggc tactcgatgg agacacccga gggttggtcc
      661 gaggtgctct accgcaacga gacttacaac ttcggtttcg cgtccttcgg caacgatgtc
      721 tatctgcggc taccaggtga caagaatccg aatgacgtcg tcatgacgct gcacgacacg
      781 tggaagtcca acgacaccgg caggctggtg ctctcaggct cgaacgtgcg tctgtcaggt
      841 ttcgagttgc ggttggtcga cgcgatgctg cgctcgccgt ccgtcaacgg cgtcatcgat
      901 cacaacctgt tctacatggg cacggtctac tacaagggcg acaagggtac gccatccagc
      961 tatcccacca accaggtcgt ccagtacaac cggatgatcg acaccagcat ccggccccgt
     1021 gatggcggca ccggcgtgat tccgtggttg ttcgtcaagc agaacctgaa gctcaacggt
     1081 aaagagacca attggcgccg gactggtgag gccgccgagc agcacgcgat tgaaggacgt
     1141 ggcggcgcca accaactcgt ggtgcgtcac aacacgatcg agggctactt caacggcgtc
     1201 ggcgccaact cgaccgactt cgaccgctac tcgaaggccg gcgccgatat ctacgacaac
     1261 gccttcagcc acatcgccga cgacacgatt gagccggaag gtgacacgat caactggcgc
     1321 gtctggtcga accgaatcga atacgcctcg gtcgccatgt cgaccggccc ggtcgacttt
     1381 ggcccgatct acttcttccg caacgaaatc tggatgctgg gcaaccaggg cgtcggcccc
     1441 gaccggtccg gtgacagcgg cgtcggctcg tacgcgttca agtactccgg cagcagcaac
     1501 ccgctggcgc gcatctacgt cgtccacaac acgttctgga cggacaaggc cggcacgaac
     1561 ggtggcctgc aggcggccgg cggaggtagc gatacggagc gcttctacct gcgcaacaac
     1621 atcttccgcg tcacgcgcta cgccttcgac tcgccgggtt cgggcggcgg ctggaatgag
     1681 gattacgact tcttcttcac gtccgatacc gggcgcggca tcaactacgg cacccggaac
     1741 tacaagacgg tctccgaata ccggaaggtc agcggccagg gcgcgcacac caatctgtcg
     1801 gacaagaccg accggttcaa ggccgaacct ggcctggtcg acccggcacg aggcaacctg
     1861 gacctggcga acggatcggc ccagatcgac gctggtgtgg tggtgccgaa catctccgac
     1921 caggcgggcg tcgactacaa cggcgcggcc ccggacctcg gagccaacga gcggtaacaa
     1981 gccgcgaccg catgcatcgc aagcgaggag cgcccggcgt tcagacgccg ggcgcgtctc
     2041 agtcctctca atcgcaatca tgcgttgtgt gcggccgttt ccggttgctt cctatcgcat
     2101 gcctgcgggg cgggttgcac gcaacgctgg tagcagccgt gccaacagtt ggcgattcag
     2161 cgagacgacc gcgccgaggt agaccgcgct cgccaggatg acgaccaaga gcagctcgaa
     2221 cgggtgcgag ccggcatccc gcaggctggg ggtggtccgg ccgagcgcga tcagcaacgc
     2281 gacaccggca acacctgcca actggccgac gataagacgc atgatccggg cgagaccgac
     2341 ttcggcaacc cgcgcgaaga agacggcatt gagtggcacg aggatggccg ccacaattgc
     2401 gtggatgccg ctgacggcat tcacgcccgc cccagcgacc agcatcatca gcggcgccga
     2461 gacgagcagt cgcgccaacg tgaacttcca gtccatgtcg ggccggccaa gcgctttgta
     2521 gaccggtggg aagacagctc cgatcgagcg caggacgccg aagatcgtca gaattcgtag
     2581 cggatcgccg atcgtcgacc acttgtcgcc cagcagcagg ctcatgtaga gcggcgcgca
     2641 gatgaaaatg accaggccgg tgccgatcga cagcgtccag acgacgacga agacatcctc
     2701 gaagagcgtc accatccgct ggcggtcctg acgcaggctc gcgtagacag ggaacatggc
     2761 cgtcctaagc acctggctga tggccgtcgt tgggatgttg gcgaccatga aggccagcgt
     2821 gtagatgccg agcgcacgcg gcccgaggac gtagccgacg atcaggtagt cgacgttgtt
     2881 ggcggcgaag ccgacgatcg cattcagtga gacgtgcttg ccgtagccga ccaactggcc
     2941 agccaacgag gcgtggtagc gcggaagcgg gtgagcgtcc gacaagtacc agagcatcag
     3001 cgtcttggtc agattaagcg tcaggaagcc gattaccagg ctccagacac cgaagccggc
     3061 cagcgccagg ccgatggcga cgacgctctc agtcgccagg gagacgatgt cgggcaacgt
     3121 ccggcggcgg aaggcaagct gcttcgagag tagcgcattc tggaccgagc cggctgccga
     3181 gaaaatgaga ccgatcgcca ggacgatgac gatcggcgcg atggtcgcgt cgccgcccaa
     3241 ccgtgccagc agcggcgcca acagcgcgct gatgcagaac aggaccacgg cagcgccgag
     3301 gttgatcgcc agtgccgttt gcagcaactc ggtggtggtg cgctggttgt agatcagcgc
     3361 atccgggacg ccgaggtcct gcaagagcgt gaaggcgccg atcacgacca tcgcgaaggc
     3421 gaccagcccg aagtgctccg gcgtcagcag ccgtgccagg acgacgttcg aggccagccc
     3481 gaataccttg ctcagtgcga acgagacgaa gatccagaca gcgccgccgg cggcccgctt
     3541 gcggatcgtg atctcggcgt cgtgggcagg atgaccaagc tccagcgtac cggcctcgac
     3601 ggttggtaac tgaccgggcc gcgcgctcat accggccggc gcccggtcat cggctgtacc
     3661 cgtagcggcg caggaacggg taggtgaggg cggtgacaag cacccggcga cggcgctcca
     3721 acccggtccg ccaggcagca tcgacccgca gcctgacgac accgatgtct gcccgcgtgt
     3781 cgctgccggc gattgaatgg ttcggccgta gtcggatctc gccgtcgccg atctccggta
     3841 ggccgatcgt cggttcggtg atgaacgtct tgatcgcctc aatcgtttga agcggtgatg
     3901 cgatcaggtc ttcgtaacga atgcgcaggt acggctggcc cgaccggcgc gtcagaatct
     3961 cctgaacgcc gttgcgcagg ttccacatca gcgcgctgac ggcgattccg gcccgccgtc
     4021 cattgagcgc gccacgctgc cggcgacgca ttcgtgagta ctggacgccg cgcggatcac
     4081 ggacgaggtg gacgacatag agatcgaccg acggcagcaa attcagcagg aagccgtaca
     4141 tcggcgactt ggaggagtcg acgatgacgc gggattgggt tacctcaccg atcgagcgat
     4201 agaagcgatc gagcatcgtc aggtacggcc cgaaggcgcg ctggacgcgg gcgctgtcac
     4261 cacccatcag catcagcggc aggtgccgca ggcgaggttg gtgagcgatc atcgcgcatg
     4321 ggtcaacccc gtcgggtcca tcgaaggcat cgttcacgac ctgttgccag gtcggacagg
     4381 accggaacgg ctcgccgcaa ccgcagagat cgttgcgcag gaacgagcgt tcccagatca
     4441 ggcgcaactc gccaggggcg aagctgtcgt ccagttcgcc cagcatctgg gtcaggatcg
     4501 tgctgccgct gcgtgtagcg ccggagatat agatcacctt gacggacggt gttgcggcgc
     4561 tcatgagttt gaagtcctgt ccccttgcac tcgtccacca tcgctcctgc gccacagcgc
     4621 cggcggtcag agcagcgctg gctgtggcac gaggccgttg gcgggcagga agttgcgggc
     4681 gaccagcgtt gccagcacct tcgtgacgct ctcctccagc gtttcgcggt cggtgtggca
     4741 ttcgacgtcg ggtagcagcg gcggctcgta gggatcgtca atgccggtga attgcttgat
     4801 ctggccggcg cgtgccttct cgtagagccc cttgacgtcc cgcgcctcgc agatctccag
     4861 tggcgcgttg acgtagacct cgacgaagtc gccgattcgc gtgcgaacct cgtcgcgggc
     4921 ggcacggtag ggcgagatcg cggcaaccag gacgatgacg ccgttgcggg tcagtagttg
     4981 cgagacgaac ccgatccggc ggatgttctc gtcgcggtcg gccttgctga atccgagccc
     5041 cttcgtcagg ttctcgcgga cgatgtcgcc gtcgaggact tcgacattca gcgcccggtc
     5101 gcggagtgcc tgcgcgacac gctggcagat cgtcgtcttg ccggcgccac tcaggcccgt
     5161 gaaccagacc gtcacgcctc gttgctgcat ggggcttgcc tctcgttcat cgcttcggtt
     5221 gctcatcggt cggcggcgac gccggttgat tgcgcgtagg cctcgatcag gattgccgcg
     5281 acttccggcc gcgagaattc gggcggcggc atctcgccct tggccagcag ctcgcgaacc
     5341 ttcgtgccgg agagatggac ccagtgctcc tggccgtgcg ggcaggtctt ggacgtgacg
     5401 agctggccgc acttcaggca gaagtggctg tgctcgaact tcagcggggt gatgccgatc
     5461 tcgtccggcg cgaagcggtc gaagatctcc tgcgaggcgt aggtgccgta gtagttgccg
     5521 acgccagcgt ggtcccgtcc aacgatgaaa tgggtgcagc cgtagttctt gcgcgagatc
     5581 gcgtgcataa tcgcctcacg cgggccggcg taacgcatcg cggccgggaa gacgctcagc
     5641 agcgtccgtt cggccgggta gtacgtgccg aggatgactt catacgtccg catccgaacg
     5701 gcagccggca catcgtcgcc tttggtcgtc ccgaccagcg ggttgacgaa caggccatcg
     5761 acgatctcca gcgcgacctt ttgcagatat tcgtgcgccc ggtggatcgg gttgcgcgtc
     5821 tggaacgcga cgaccgtccg ccagccccgt cgggcaaatt cggcccgtgt ctcgactggc
     5881 gtcaggcggc actcgaagaa gtcgtcgtgg ggaatgcgat cgattagcag gatcgagccg
     5941 ccgagacaga tgtcgccggc ggcgtagacg gccgcgacgc cagggtgggc ggattcggtc
     6001 gtcccgtaga cattttgcgc ctcgcgcggc ttgtcacgct ggtagatgtc ggtgacggtc
     6061 atgatcgcca gcggcctgcc gccgtgcgtc agggcgacct gccgatccag cccgatccgc
     6121 ctggcctgat cggcaccgac cggcagcgtg acagggatcg tccagggcag gccgttcgcc
     6181 agccgcatat catcgacgat cgagtcgtag tcagccttgc cggcgaagcc ggtgtatggc
     6241 gagtagatgc cggtcgcgat gcactccagg tcagccagcg cgcgtgcgtc gagctcgatc
     6301 gtcggcagcg ccgccgctga cgcccgcaac gccggctgga cctcggttgg cgcgatctgg
     6361 ttgaccagcg tcccgccgtg gggcgcgatc agttcccggc gcgtcacgtc ggtccggggt
     6421 gccgtctgtt gatccagcat cgaaataccg tgcctatccg tggttgattc gcccctgcct
     6481 tgcgtgcgaa cgtgaacgca tcgttggcgt ttgggcaagc cggtgcaaac agctttgact
     6541 ctacgctgga gtcggcggca aaccgtcgcc ggccggttga gattcggtca aagcactgac
     6601 gttagccggc atagccgtag cgatgcagca gcggcagcgt cagccggcgc acgagtgtcc
     6661 gtgtgtcggc gtcgaggtcg gttcgccagc gctcgtcgag ccgtagcggc acggcgccgg
     6721 tctcgatccg gttgcggttg ccgcagacgg tgtggtttgg gctgagatag acggtgccgt
     6781 ccttgaagcg cggtatgccg atgtccggct cgtcgaggaa ctgcttgatc tgctcgatgc
     6841 tggcgaccgg gtccgcgacg aaatcttcgt agcgcatgcg catgtagcgc ttgcgctggc
     6901 gcaggccgag cagctcctgg gtggcgtgca aggcgtccca ttcgagcagg ctcctggaga
     6961 cgctgtaggt gtcgagcgtg ccgagcatcc ggtcgccatc gcgggcgcgg cgcaggttcg
     7021 agctctggac gccgtgcggg ttgcgaatga gcaggagcac ccgcagatcg accgacggcg
     7081 cgaggccgag gacggccgcg tagagcggcg tcttcgacga atcgacgatg acgcggctgt
     7141 cggtgaccgt tgcgattgat cggtagagtt ggtcaagcgt cgcaatgtac tcgcggaaca
     7201 tccgagcgat gaccggctga ctacccggca tcagcagcag cggcgtgtgg cggcgctttg
     7261 gccggaggtc gatcatccgc tgccagtcga cgttgtcata gccaccgtac gcgtcggcga
     7321 tgatctcctg ccagaggtcg cactcgcgga agccctgacc gcagccgcac tcctgattgg
     7381 tctggaatga gcggtgccag atgccgcgca attcggcggc ggcgaacgtg tgctccagct
     7441 ctccggccac cttggtcagc agtgtgctgc cgctgcgctc gaatccggcg atatagagca
     7501 ccttgaccgg cgtctcgact gccatcgtcc aggtggtcct ttcgtgtttc aggcggcgtg
     7561 atgacactcc caacctattc agcgcatgtt tcgcagacgt tgccggttgg ttgaaatcat
     7621 gtcagggcgc tgcctagtgc atccagcacc tggtcgcgga gttccgaccg gcagatcagc
     7681 aggtcgggca gccaggagtc gggctggttg tagcgcagcg gcgtgccgtc aatccgcgag
     7741 acgtgcaggc cggcggcagc cgcgacggcg accggcgcgg ctgaatccca ctcgtgctgg
     7801 ccgccgctgt ggacgtagat gtcggcctcg ccgaggacga cggccatcgc cttcgcgccg
     7861 gccgagccca tttccagcag gtcggctccg agccgctcct ctagatcgag cgcgacggcc
     7921 ggcgggcggg tccggctgac gacgatccgg gctggtccgg tccgccgatc cgggagcatg
     7981 ggcggctctg ccgttgagag cgtcatccca cgcgccggca gggcgacggc gccggccaac
     8041 ggctgctcat cgatcaccag tgcgacgtgg acggcccagt cggttcggcc cggctcgccg
     8101 tactcgcggg tgccgtccag cggatcgatg atccagaccc tccggctgtc gagccgcgct
     8161 ccgtcatcgt tggcttcctc ggagagaatg ggatcgttgg ggaagctggc ggtcagtgcc
     8221 cgcatcagca tgtcgtgtga gcgatggtcg gctttgtcgc ggagaacctg cggcgcgatc
     8281 tcctgccgtg ccgcccgttc acggagcgcg agcaacacct gacctgcctc ggtcgcgatc
     8341 cgggccgcag cgtcatgctc gaaacacgcg ggcatcgtgt gcgtctcccg tttcaatcag
     8401 tatccgtcaa ccagtaccgg cggtccggca gtggatctcc ggcccgatag aagtcgatgt
     8461 cgaagcagtc gaggatctcc atcgcccgtc gctcgtcggc atcgctgacc tgatcgcgcc
     8521 atttctcgac caggcgttgc gctcgtgttg gcgcgtcggc gttgaggagt tttcgggtcg
     8581 cgctatcgga tttgcgtgac gactgcgacg gcgtcgcgat tgcgtcgagc atgcgttgcg
     8641 ggttaggcag ttccagcttg gcggcaaggt gtcggacaac cgtcgcgggg tcgaggatca
     8701 gctgttcgta gctggcgatg acccagtcgt cggtggcatc gtgaagtggg actgaagtct
     8761 gaaggcacca ggacaggacg cctctctcca gcttcgaacc gcttcgaacg atatcagcgg
     8821 cgaactccag ttgctcgcgg gtgaaatggc tgcggtatgg cccatcgagc atggcaggca
     8881 aggttggcag gacctcgcgc gagaggctga cggcgaccgg atggcggacg agatagacga
     8941 tccggccgtc gaaggtgtcc cgcagccagt ttagccgtcc ctcgcagccg tggatgacct
     9001 tgaagacgat ccggctcgtc gccggccggt agtagcggcg gaacggacgt gggttgagga
     9061 agcccagctg gccgttgcag agacgatcga agtagtggaa caggcgctct ccggcatcgt
     9121 cggaatagag ctggccccac tcggtgatgc cgaggtgctt gcggacatac ggattccgta
     9181 gatcggtcgg ctcgtcgcag ggcttgaagc ccggctggct ccagatcagt tccatcagcc
     9241 aggttgagcc gctgcggggt agcgacgcga cgaacacgtt cggcaggctg ccggggcggt
     9301 ggatgttgac gaacgggcgc gttgcatgga cgagcgcggc agttcggcta acgcggtatg
     9361 acaccagcgg gacgtcgtcc ttcactgcaa ccgctgcctg cacggtctta cctatccgtt
     9421 cgttaacgct ccagggcgcc tacgacgcct tcatcgtcac gagccgtccg tcggcgtcga
     9481 caacggccag tgatcgccgg agcagctggg tgtacagctc ctcgtagctg ccgatcatct
     9541 gcgccaggct gaaccgggcg tcgatcagtg tccgcgctcg gcttgccatc tcgcgggctt
     9601 cgacgggttc ctcgatgatg cggaccagcg cgctggccag cccggttgga tcatcgggga
     9661 tgacgagctg accagtctcg cccggtttga ccagttcggg gttgccgccg acgtcggtcg
     9721 cgatgaccgg cacaccgagc gccatcgctt cgaggatcga gttcgagcag ccttcgttgt
     9781 cgcgcgagga agagatcagc acgtcgaagg ccgccaggaa gtcggagacg ttgcgctggt
     9841 agccgaagaa cgtcacggcg tcggtcagtc ccagctcgac caccagcgcc tcaagttcgg
     9901 agcgcagcgg cccatcaccg acgattgcga gcttgactgg catttggtgc tcgcggagcg
     9961 tcgccagcgc gcgaattgcc gtcgaatggc ccttggccgg cgtcaggctg gcgagcatcc
    10021 cggcgacaca cgcctgcccg tccggcgcga tccgcgcccg gtgggcagcg gctgcactgg
    10081 cgtcaacggt cagccggtcg aggttgatgc cgttatagat gacgcggatc ttctcgcgtg
    10141 gcaaaccccg cctgaccagc atttcggccc cggcggcgct gttggggacg atcgcgtcgg
    10201 cgaggcggct gaaaacgtct tcgaccgtcc ggtaggtctt gtagccaact ccgcgcgact
    10261 ggcgcacgcc gcagcgctcg gtgacgatcg tcgtcgtcgc gttgcgggtc agcgccggta
    10321 gcagcccgaa gaacgtcgcc gggctgagga acggctggac gatatcgacc cggcggctgc
    10381 gaagcagtcg agtgacggtc cagagcggcc tcagatcgta cttgctgcgc cggttgaggt
    10441 cgattacttc gatgcccggg acggcccgga actcgtcctc cagccctcct cccggataga
    10501 gcggcgcgac gatcggctgg aactgttcct tgtcgagccc acgaaccagt tcgagcaact
    10561 gctgctcggc gccgccgatg atgagcttgt tgaccggata gaggatcgta tacatcacgg
    10621 cgctcccagt ccggtttgct ggcgctgccg gcgcatcgac gcggcgtccg cccgcgccag
    10681 ccgtgcgagc gcgatcgaga ttcccgcgta gagccagagc gacatgctgc tgtccagcga
    10741 gttgtggtag gcggagttga cgcagtaggc gaccagtccg ccgatgcagc cgatcgccag
    10801 cccgcgaacg aagcggtcgc gatggcgggc cgcgattcgc agattacgga aggtcgcgac
    10861 gatcagcgcg atgtagagcg ccagcgcgac gatgccccac gagagccaga tgttccagta
    10921 atcattgtga ggttcctgct ggccgagggc agacgctccg ccgtcggcgg atatctgcac
    10981 ggcgatatca tcaatgtagt cgagtgagac gtccgtgaac gcctcgtggc cgatgccgtc
    11041 cagcgggtgg tcaagcgcga tggcgaggct gacgtcctgc agcgcctggt ggctggcggc
    11101 actgcggtcc tcatcggagc cctcgtagta acgcgacccg aacacgccgc tgttttcgag
    11161 cccgaagaag aggacgatga cgacgatcac ggccgagatc atcagcgtcc ggcgctgccc
    11221 gccgaacagc aagcccatca cgaccagggc cggggccaac gcgaacagcg ccgagcgcgt
    11281 gtaggtgaag ttcagtgctg ccagcgtcag gacggtcgag ccggccagca gcagatagcc
    11341 gcgccgcagc cgcagcggct tcgcgatcag tacgccgagc atcggcgcca gtacgaagat
    11401 cagctgggtc gcgatcggca ccggtccctt cgtcagaccg acggcccggc ccttgacggt
    11461 gtcgtcgcgc ggcccgtaca gcgcagtggc cggcgcgtag tgctgccaga tgacgatcag
    11521 cgcgacgccg gttgtgacga ccgccaccac cagcgcgatc cgcttcatgt cagggatcgt
    11581 gtagagcaca caggccgcga tgacgagaat cgcgaactgg accaattggt agcgaaggta
    11641 ctggtagacg tatccgtcgt ccgagtgctg gatgttcatc agcgtgacga tcgccagaaa
    11701 gccgattgcc ggcaggacga cccgtcgcgc ataccgatct ccggacaggc gggcgcggaa
    11761 gatggccggc agcgcgatcg cgagggcgat ggcggcgatc acgaaccgtt gggcgatccc
    11821 gaacgtcgga ttcgtgaagc catacagcgt catctcgttg atcaggatcg gcgccagcac
    11881 ccaggctggt ccatacatcg ccaggatgaa ggtggcgaag atgaacgcag tcagccaggc
    11941 ggcggtcagg ttgcccgact cggcgaagac gaacgacaac agcgctgtcg ctgccagtat
    12001 cggcagcagc agccggcgga acgagaactc cgccgccctg ccggatgctg cccagatgcc
    12061 gcgtgatggt ctgcctgcga tcgtgtcgtt cattaccatc gttcgccgct gtccaactct
    12121 cgtacgctcg cgctagtcac cggccggcgt cgggttgccg gcagggtggc ttccgctccg
    12181 gacgctccgg ttgccgccgt gatcgttgcg gaccgacagc tcatcgacga gcgcaaccag
    12241 ccgctcgaaa tctggagtgg ccgtgctggt aaccgggtag cgtccgtgct tgcgggcgcg
    12301 ttcggcgcgc ctgcttcgca gaagatcgag ccctaacccg agcgcggccg cgacggccag
    12361 tcctccggcg atcccgatca ctcccagttt cttcatgccc atcgtcgcga atggtgtcga
    12421 ggcatcgcca tagagcatca ccggtgcccc ttgtgtgagc gcggtgacct gaccggcgac
    12481 gaattgctcc gtcagcgttg agagctgtag cgagtaggca ttcgagaacg ctgccgacag
    12541 cgaaccgtcc tcatcagccg ggagcttggc gatatcggcc tggacggtgc tgagttcgcc
    12601 ctggatcgat tcgatgttgg cctgggtatc gctgatctgc tggctgatcg cgcgcaagag
    12661 ctgcgtgcgg acctcggcct gctgggccag cgcgtcctcg gagaagctgc tgcgatcgcc
    12721 gatggcgtcc gactccgcct gccggccaag ctcctcctgg tagttggtct gcaggtccag
    12781 caacgccgac tgccgatcac gcagctcgtc ggtgaggtcg atttcccgct ggcgcagggc
    12841 ggacgttgcc agctgctggc gggtgaactc gatctgctgg ttcacctg
//
LOCUS       DASUGJ010000613        13440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1905418, whole genome shotgun sequence.
ACCESSION   DASUGJ010000613 DASUGJ010000000
VERSION     DASUGJ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13440
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905418"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1443
                     /locus_tag="VFV93_13970"
     CDS             160..1443
                     /locus_tag="VFV93_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166505.1"
                     /translation="MRFVTRRMLVALMLVVLLLPAVIACGGGDDDDENPTATTSGEAA
                     TATEATAGEATATAAGDADATGTSPSAATSTTGTDATTPPTGDSAFAYGWNVAVRGDD
                     GGAEHNVKTIEAVQDSGFGWVRFQLEWNQFERADNQWDPLPIDRKVDQIAGSNLKILL
                     VVAKAPDWALDPSGNQLLADYGQFQELMQFVSQRYAGKVQAWEIWNEQNLAYEVNGTV
                     RVNDYVDLLKAGYEGVRAGDPNALVVFGGLTPNGVNDPSVAIDDVEYLTQIYQVNGGE
                     IANYFDVLGMHVSSTHNPPDTMWPDNPSDEEGYNDDESFYFRRAEQLWDVLVANGDSA
                     KPVWITEFGWTTANQAPGYEYGVNNTEQEVADYLVRSFEIARTEWSFVTGMFVWNLNW
                     STLTTPDDEKYPWSALNEDWTPRPAYDALKAMPKT"
     gene            complement(1571..2395)
                     /locus_tag="VFV93_13975"
     CDS             complement(1571..2395)
                     /locus_tag="VFV93_13975"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX5166506.1"
                     /translation="MSRGRLRYTAPPSRRIEPISQATRALVIVPTYCERRNIAELIPA
                     ILAHDTIEVLVVDDGSPDGTADAVRRLSARHAGRVHLIEREGKLGLGTAYVDGFRWAL
                     TRDYTHMIEMDADLSHHPASLPRLLHASRQADLVIGSRYVPGGRTVNWPGYRKLISRA
                     GSIYARSLLNLPVRDLTGGFKCFRRNVLESIDLDSVNSTGYAFQIELTYRAANRGFRI
                     AEIPITFAERTHGTSKMDAAIVVEAVLCVLRLRAGATQPARPLVRPFRSPGESPGV"
     gene            complement(2509..2778)
                     /locus_tag="VFV93_13980"
     CDS             complement(2509..2778)
                     /locus_tag="VFV93_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166507.1"
                     /translation="MLRRLLVGLIACAGIAAGGWALLQRANRSPAGEPDAVQWPFDTE
                     ELRSLMGGVVAEQRDRINAIEDEAGRDRARSFLAYYERRRQAVAS"
     gene            2948..3997
                     /locus_tag="VFV93_13985"
     CDS             2948..3997
                     /locus_tag="VFV93_13985"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5166508.1"
                     /translation="MGKYARIVLGTAATVFFLWLALRNVDISAVGDALETANYYFLIP
                     AALCTVGGFCVRTLRWSFILRSSKAIPFQRLFPVMMVGFAANNLLPARIGEFVRAFLV
                     GSREGVSRSTALATIVVERVCDGLTLLALMTLTLLLFPIPMDDSKLQAVVIGASLIFG
                     VATVALLGLILFPTRMLVPIRFGTRRLPERFADRADALIDAFLEGLHALRDARSLLGL
                     SALSVGIWALECATFAFVLRAFPLGLSTGEWLAAAAFLLVFVNLGIMVPSVPGYIGTY
                     QFFATLALSAFAVNDDYAFGLAVVAHAMQYTLITGIGLICLWRLGLRIGNVTRIAPER
                     ATAPAPLLSRAPDQS"
     gene            4158..5699
                     /locus_tag="VFV93_13990"
     CDS             4158..5699
                     /locus_tag="VFV93_13990"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5166509.1"
                     /translation="MQTAERSTIVMGAGPGGLATGYWLAKHGVPVTVLERAPFVGGLA
                     RTVQQDGFKFDIGGHRWFSKVDEVNDFYKEIIADETVWVRRISRIYFDGKYIDYPLEI
                     SNALAAIGPVKAAQAVIDYGRSAWARRSRTTPIETMEDAYVDQYGRTLYELFFERYSE
                     KVWGRPCSEMSGDWVSQRTKGMSLVTAVKDAIIPSKGAVQSLIESFMYPRYGYGRLSE
                     RMAEGIEAAGNEVRLGAGVTKVLRQGNHITGVVVTDDQGRESIVEGANFVSSIPLTVL
                     CSIMEPAAPSEVIEAAKSLTFRNVITVNLMLDREKVTDDTWLYVHDRSIKFGRLHEPK
                     NWSVDMVPDQSKTSIVAEYFCSFGDETWSMSDDALVEQTANHLVDDLGFVERNEVLGG
                     FTVRAPRAYPSYTSGYEAPLALLKEFVGSFDNLQIIGRYGTFRYNNADHSVETGLLAA
                     RNILGESYDLDQVNAEKEYQEIKRGNMRVSRRVRIVETQPDEMPAEVPAELVTSDASQ
                     PAPAK"
     gene            5721..7145
                     /locus_tag="VFV93_13995"
     CDS             5721..7145
                     /locus_tag="VFV93_13995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5166510.1"
                     /translation="MPEPATAAPGTRERVTTDRRSRSAPETLALARTSVAGSASQTTT
                     WIIVTLLAVTLVKQLVLVVSYPPFEGHDEVAHYGYVATLDRERRLPTLADNLPPETGP
                     YSRFTLDWPALYTANHPPLFYMLALPLYRLARPDVEARLYAMRLLSVPLFLLVIWLTW
                     RLALLLFPGDLFLAVTAPALVAFQPQLSFEGAIVNNDMLSIVFGAALLYLLAEALRRG
                     LTTERALLIGAVTGVGLLAKATVTVFIPLAIAVAVWSAYRNRYGPGRSGWWPDAFRGS
                     LLICVPALLISLPWYVFVYATYGDLTVFRSLDLLQSSWGTPAASLFDLLLSPHFQSER
                     FHEGWGYFGWRLLPLGSFELAFAWIVSIVAVAGLAVEGWRCWRRRASGAIDPDLVAAL
                     VMLLACCTLFYLAMVYFGTRVAFTQMRYVFPAAPAAALLAASGYRALFANRWRPAGAT
                     AVVALALLFQILILTRIVMPHAMV"
     gene            complement(7183..8238)
                     /locus_tag="VFV93_14000"
     CDS             complement(7183..8238)
                     /locus_tag="VFV93_14000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX5166511.1"
                     /translation="MNVKTALIVAIVTIDIVVIAFALPLPGRAEVEAAMPSGIAVDPA
                     GQRVYVAMSGADAVTVIDSTSRQVFATVPVGRQPVGIVVDERVGRVFVANRLDASVSV
                     IDTSSLAVVATVAVGDYPWAIAQNERTGKVYVTNRRDGSVSVINDATLAVVATIPVQD
                     DPAGIALDPVLARGYVTNRESGTISIIDTDENQLIENIDGGSYAWGNAVDPRNGRVYV
                     AMMGSGTVDVIDGPAGRVAILPAPFDPQGVGLPSANFDTYFSITVGGDPSDAAINPIT
                     QRVYVTNRRYSTVSVIDAVANHGITTIPVMLDPIAVAVDPTTNLIYVASRRKDAVTVI
                     DGATNLVVTDILLDGDV"
     gene            8336..9772
                     /locus_tag="VFV93_14005"
     CDS             8336..9772
                     /locus_tag="VFV93_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166512.1"
                     /translation="MALLEQLEQAGPNRQQATGQDTLPSDAVQAPRHSYSIGLRTRIV
                     LALIAIACVKQVVLAVAYPPFEGHDEVAHLGYLATLADDGRLPTFSDDLPASLEEYSR
                     FTLDWPAVYTANHPPLYYVVSVPAYKLAGSDHLAQLYAVRLVSIPFFLLTVWLTYLTA
                     RMLFPTDNLIVLGAPAIVAFQPQLGFEGAIVNNDMLAIVFGTLLLYLCLRAIRDGLSI
                     RLALIIGLVCGLGLLTKATLLALLPIVAAVCVLLCWPRPWTRVRSRDWWQSTLVRALV
                     VVGPAVALALPWYLYLRRTYGDFTAFEATQELQRNWNHPAGTLTELLFSPLFHKERIH
                     ETWGYYGWRLIPLDSRELAVVYVMMTLAGLGALLGLLRWFGHVRDNGWPGRTTFAGQQ
                     ATSITTLTAASLLMYGAMVYFGTMFLLTQARYFFPVLPVAIVLATAGLRVLVPEGWYR
                     AATIVLVGGAVGFQFLILTKLVLPYAYL"
     gene            9801..10052
                     /locus_tag="VFV93_14010"
     CDS             9801..10052
                     /locus_tag="VFV93_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166513.1"
                     /translation="MTAGLTAIVTLVLLGLALLIPLDTRNARGVEQQKTAFDIWRRTL
                     EHVPADAPFFLTTATLTVLSLAALVASAWVLVAIARLPR"
     gene            10092..12239
                     /locus_tag="VFV93_14015"
     CDS             10092..12239
                     /locus_tag="VFV93_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166514.1"
                     /translation="MATTPSLSAKPSPNAGARPRTDQSNPAGRLRGMLISRRWEIALL
                     ALIAVVAIQVGALDWYTSYPAFDGSGDKLPNTFASIDHPFHINKEQAVLNALRDGQFP
                     GWISNHQGGYPTEFYPLGADLVVATGWAFSLGQLPLEIVHKLVVIAVLFIPVLAYWAI
                     ARRDRLPLGIAVTAGLLHVFLPGNWLGGGPDELLRMGMWPNVFATYLTLPLILWAAGY
                     LRHGSRRSFVLAAVVASLAIYSNPRSTIAVATALLAVGIVALLERRESCPRQSRTRQK
                     WRTDIASIRPLVRRSVLLALTIGLLCSALLVPLRAHQHLYEFSHFVEFDHPGQIWSYY
                     RQAVPTEIIVLALLGMVIGLARRDFYTRVLAVWLPLSYTVILIAGWLLRDLSIFAQLE
                     GPRLIPLLRLPSVFLAAVALREVVRFVIRLLRADRLLPATNIAAVTIVALAVLTPVSM
                     LEDDQRGLPDLETTDQPDFAAIARSARIYQQATTPDDRPFVIGSTISEHAAFWIPVLT
                     GDDAFLSNWVWNWRIPDYANRTQIADVASALRLDFLRRHALTMVLISTDSDATLNVAR
                     ASPYLDELDPGTAGGYAIFRVDDPGPKTRGPVTAGAGTVTALDQSREHLRATIETTQP
                     TEVTIAINAFPAWHATVNGQATRLNRTADGSMHLTVPAGTSALELRYTTEPAVWAGRI
                     LVTLGILLMIAVVLAPRLPKPPRLASRVSRHNP"
     gene            12290..>13440
                     /locus_tag="VFV93_14020"
     CDS             12290..>13440
                     /locus_tag="VFV93_14020"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5166515.1"
                     /translation="MTEDQGPHARRIALSFDVGGTFTDFVAIDVAAGEVAGRHKVLTN
                     ARYPARGVVRGWQEMVEAGLTAEAIQLSVHSTTLVTNALIERKGAKTILLATAGFRDL
                     LELAREQMYDIYDLFAPPPEPLIPRPLRLEIDERTDADGQILREPSPESIEAAIATIR
                     ESGATAVAIAFLHAYRNPANEQAVAAAIQQALPDVAISLSSAVAPIVGEYERTSTTAA
                     DAFAKPSVRAYVGELDDSLAAGGIRAPLYLMLSSGEIASAATAIERPIRLLESGPAAG
                     GIAAAFFGRLSGHDNVLSLDMGGTTAKACVIEAGKPELAHMLEVARVRRFMAGSGLPV
                     VAPVVDLIEIGAGGGSIARRDELGLLKVGPDSSGADPGPACYGLGGELPT"
BASE COUNT         2113 a         4625 c         4235 g         2467 t
ORIGIN      
        1 actcgtaacc aaccccgccc ctccaacgtt ctgcggtggg agtgacgtgc ggcggaccaa
       61 gagtgtccgt acggtacact caaccgttta cgcgggttgg ctgttgccta tccgtgtgct
      121 ggatgaggac caccggagac cttggagtca ggagagttca tgcgttttgt aacgcgaaga
      181 atgcttgtcg cgctgatgct ggttgtattg ctgctgccgg ccgtgatcgc ctgcggcggt
      241 ggcgatgacg atgacgagaa tccaaccgcc acaaccagcg gcgaagcagc gacggcgacc
      301 gaggcaaccg caggtgaggc gaccgcgaca gccgccggcg atgcagacgc gaccggcacc
      361 agcccatcag cggcgaccag tacgactggt actgacgcca cgacaccacc aaccggcgat
      421 agcgccttcg cctacggctg gaacgtcgcc gttcggggtg acgacggcgg cgcggagcac
      481 aacgtcaaga cgatcgaagc tgttcaggac tccggtttcg gctgggtgcg cttccagctc
      541 gaatggaacc agttcgagcg ggccgacaac cagtgggacc cgttgccgat cgatcgcaag
      601 gtcgaccaga tcgctggatc gaacctcaag atcctgctgg tcgttgccaa ggctccggac
      661 tgggcgctcg atccctccgg caaccagctt ctggccgact atgggcagtt ccaggagttg
      721 atgcagttcg tttcgcagcg ttatgccggc aaggttcaag cctgggagat ctggaacgag
      781 cagaatctgg cctacgaggt caatggcact gtccgcgtca acgactacgt cgacctgctc
      841 aaggccggct acgagggcgt tcgcgctggt gatccgaacg cgctggtcgt cttcggcggt
      901 ctgactccga atggcgtcaa cgatccctcg gtcgcaatcg acgacgtgga gtacctgacg
      961 cagatctacc aagtcaacgg cggcgagatc gccaactact tcgacgtgct gggtatgcac
     1021 gtctccagca cccacaaccc gcccgacacg atgtggcccg acaacccgag cgacgaagag
     1081 ggctacaacg acgacgagag cttctacttc cgccgcgccg agcagctctg ggatgtgctg
     1141 gtcgccaacg gcgactccgc caagcccgtc tggatcaccg agttcggctg gacgaccgcc
     1201 aaccaggctc ccggttatga atacggcgtc aacaacaccg agcaggaagt ggccgactac
     1261 ctcgttcgct cgttcgaaat cgcccggacc gagtggagct tcgtcaccgg catgttcgtc
     1321 tggaacctga actggtcaac gctgacgacg cccgacgacg agaagtaccc ctggtccgcc
     1381 ctcaacgagg actggacccc acgccccgcc tacgacgcgc tgaaggcaat gccgaagacg
     1441 taacgcgacg ggcttccgcc gtcccaatcc ggtaggggcg aggcgttcac tcgcccggct
     1501 ccccgcccga ctcgctcgga ggagatacga ggggcgagtc agtgcctcgc ccctaccgga
     1561 gttcgggaag tcacacgccc ggcgattccc ccggactcct gaacgggcgc accagcggtc
     1621 gggctggttg cgtcgcacct gctcgcaatc gcaggacgca gaggaccgcc tcgacgacga
     1681 tcgcggcgtc catcttggat gtgccgtgcg tgcgctcggc aaacgtgatc gggatttcgg
     1741 cgatccgaaa gccgcggttg gcggcccggt aggtcagctc gatctggaag gcgtagccgg
     1801 tcgagttgac cgagtcgagg tcgatcgact ccagcacgtt gcggcggaag cacttgaagc
     1861 cgccggtcag gtcgcgaacc ggcaggttca gcaggctacg cgcgtagatc gagccggcgc
     1921 gcgagatcag cttgcgatag cccggccagt tgacggtccg gccgccaggc acgtagcgcg
     1981 agccgatcac gaggtccgcc tggcggctgg cgtgcagcag gcgtggcagg gacgctgggt
     2041 ggtgtgagag gtcggcgtcc atctcgatca tgtgcgtgta gtcgcgtgtc agtgcccagc
     2101 ggaagccgtc cacgtaggcg gtgccaagcc cgagcttgcc ctcgcgttcg atcaggtgaa
     2161 cccgaccggc gtgtcgcgcg gacagcctgc gaacggcatc ggccgtccca tccggcgagc
     2221 cgtcgtcaac gaccagcacc tcgatcgtgt catgcgccag gatcgccgga atcagctcgg
     2281 cgatgttccg tcgctcgcag taggttggga cgatcaccag cgcgcgggtc gcctggctga
     2341 tcggctcgat ccgtcgcgac ggtggtgctg tgtaacgcag ccgtcctctg gacatccagg
     2401 tctccgtcat tccctcaggc cggcgcacga acaggcgcga tgtagctcaa agcataaccc
     2461 gcgccgtaac cgtgcgctcc aatcgggatt cgagcaagag caagccgcct acgaggcaac
     2521 cgcctgtcgc cgtcgctcgt agtaggcaag gaaggagcgc gccctgtccc gaccagcctc
     2581 atcctcgatc gcgttgatcc ggtcgcgctg ctcggcaacg acaccgccca tcagcgaccg
     2641 gagctcttcc gtgtcgaacg gccactggac ggcatcaggc tctccggctg gtgaccggtt
     2701 cgcgcgctgg agtaatgccc agccgcctgc cgctattccc gcgcaagcga tcaacccaac
     2761 caacaatcta cgaagcattg cctttcctcc tcattcaaca ggcaaccggc gtctgccctg
     2821 gttcctgcgg caggcccggc caccagcgac tcgcaatccg atgaccacgt tatcgtcccg
     2881 gcctgcggcc cggctcttgc ggaagcccag cccgctgcca gcggacggct agtgaggtgg
     2941 acctcgattg ggtaagtacg cgcgaattgt tctgggtacg gcggccactg tcttcttcct
     3001 gtggctggcg ctccgcaacg tcgatatctc ggcggttggg gatgcgcttg agacagccaa
     3061 ctactacttc ctgatcccgg ccgcactctg taccgtgggt ggcttctgcg tgcggacgct
     3121 gcgctggtcg ttcatcctgc ggtcgtcgaa ggccatcccg ttccagcgcc tgtttccggt
     3181 catgatggtt ggctttgccg ccaacaacct gctgccggcc cgcatcggcg agttcgtgcg
     3241 cgccttcctg gtcggcagcc gtgaaggcgt cagccgcagc accgctctgg cgacgatcgt
     3301 cgtcgagcgc gtctgcgacg ggctgacact gctcgcgctg atgacgttga cgctgctgct
     3361 cttcccgatt ccgatggacg attcgaagct tcaggcagtc gtcatcggcg cgtccttgat
     3421 cttcggcgtc gcgactgtcg ccctgctcgg gctaatcctc ttcccaaccc gaatgctggt
     3481 gccgatccgc ttcgggaccc gccggctgcc tgaacggttc gcggaccggg ccgatgccct
     3541 gatcgacgcc ttcctggagg ggctgcatgc gctgcgcgac gctcgctcgc tgctcggcct
     3601 gtcggcgctc tcggtgggta tctgggcgct ggagtgcgcg acgttcgcct tcgtcctgcg
     3661 ggccttcccg cttgggttgt ccaccggtga atggctggct gcggctgcat tcctgctggt
     3721 cttcgtcaat ctcggcatca tggttccgtc ggttcccggc tacatcggca cctaccagtt
     3781 cttcgcgacg ctggcgctga gcgccttcgc agtcaacgac gactatgcct tcggactggc
     3841 cgtcgtcgcc cacgcgatgc agtacacgct gattaccgga atcgggctga tctgcctctg
     3901 gcggcttggc ctgcggatcg gcaatgtcac gcggattgcg ccggaacggg cgaccgctcc
     3961 ggctccgcta ctcagccgcg cgcccgacca atcgtgacac ggctggcacg tgagttgctt
     4021 gccgcaagcg gttggtttcc gaacgtccac ctcactgctc gaaccgtgtg cggtgtgacg
     4081 ttgggactgg cagctgaatt gatacagaag accgaaacgc caagcccgtt tggcgtatcg
     4141 caatgagggg tatccagatg cagacagcgg aacgttcaac gatcgttatg ggcgccgggc
     4201 cgggtggcct cgcaaccggc tactggctgg cgaagcacgg tgtcccggtg acggtcctgg
     4261 agcgcgcgcc gttcgtcggc ggcctggctc ggaccgttca gcaggacggc ttcaagttcg
     4321 acatcggcgg ccatcgctgg ttcagcaagg tcgatgaggt caacgacttc tacaaggaga
     4381 tcatcgccga cgagaccgtc tgggtccggc gcatcagccg gatctatttc gacggcaagt
     4441 acatcgacta cccgctagag atcagtaacg cgctggccgc gatcggcccg gtcaaggccg
     4501 ctcaggccgt catcgactat ggccgctcgg cctgggcgcg ccggagccgg acgacgccga
     4561 tcgagacgat ggaggacgcc tacgtcgatc aatatggccg cacgctctac gagctgttct
     4621 tcgagcgtta ctcggagaag gtctggggcc ggccctgctc ggagatgagc ggtgactggg
     4681 tctcgcagcg cacgaagggc atgtcgctgg tgacggccgt caaggacgcc atcatcccgt
     4741 cgaagggcgc cgtccagagc ctgatcgagt cgttcatgta cccgcgctac ggctacgggc
     4801 ggctctccga gcgaatggcc gaagggatag aggcggccgg caacgaggtg cggctcggcg
     4861 ccggcgtcac gaaggtgctg cggcagggca accacatcac cggcgtcgtc gtgactgatg
     4921 atcagggccg cgagtcgatc gttgagggcg ccaacttcgt ctcgtcgatc ccgctgacgg
     4981 tgctttgctc gatcatggaa ccggccgcgc cgtccgaggt cattgaggcc gccaagtcgc
     5041 tgacctttcg caacgtcatc accgtcaacc tgatgctcga ccgcgagaaa gtcaccgacg
     5101 acacctggct gtacgtccat gaccgctcga tcaagttcgg ccggctccac gagccgaaga
     5161 actggagcgt cgacatggtc ccggaccagt cgaagacgtc gatcgtcgcc gagtacttct
     5221 gctcgttcgg cgacgagacc tggtcgatga gcgacgacgc gctggtcgag cagaccgcca
     5281 accacctggt cgacgatctc ggcttcgtcg agcgcaacga ggtcctcggt ggcttcaccg
     5341 tccgcgcccc gcgggcctac ccgagctaca ccagcggcta tgaagcgccg ctggcgctgc
     5401 tgaaggagtt cgtcggctcg ttcgacaatc tccagatcat cggccgctac gggacgttcc
     5461 gctacaacaa tgccgaccac tcggtcgaga ctggtctgct ggcggcccgc aacatcctcg
     5521 gcgagtcgta cgatctcgat caggtcaacg ccgagaagga gtatcaggag atcaagcgcg
     5581 gcaacatgcg ggtctcgcgc cgggtccgga tcgtcgagac ccagcctgac gagatgccgg
     5641 ctgaggttcc agccgagctc gtcaccagcg acgcgagcca gccggccccc gcgaagtagc
     5701 cggaacggga cggtcgtccc atgccggagc ctgccacggc cgcgccaggc acccgcgagc
     5761 gtgtcaccac cgatcgtcgt tcccgatcgg cgcctgagac gctcgcgctg gctcgcacgt
     5821 cggtagccgg tagtgccagc cagacgacaa cctggatcat cgtcacgctg ttggccgtga
     5881 cgctcgtgaa gcagctcgta ctcgttgtca gctacccgcc attcgagggt cacgacgagg
     5941 tcgctcatta tggctacgtc gcgaccctcg atcgcgagcg ccgcttgccg acgctggccg
     6001 acaatctgcc gcccgagacc ggcccgtact cgcgcttcac gctcgactgg ccggccctct
     6061 acaccgccaa ccacccgccg ctcttctaca tgctggcctt gccgctctac cggctggcga
     6121 gacccgatgt cgaggcacgg ctctacgcga tgcggctgct ctcggtcccg ctcttcctgc
     6181 tggtgatctg gctgacctgg cggctcgcgc tgctgctctt tcccggcgac ctgttcctgg
     6241 ccgtgacggc gccggcgctg gtcgcgttcc agccgcagct cagcttcgag ggcgccatcg
     6301 tcaacaacga catgctctcg atcgtgttcg gcgcggccct gctctacctg ctggccgagg
     6361 cgcttcgccg cgggctgacg accgagcggg cgctgctgat cggcgccgtc accggcgtcg
     6421 gcctgctggc caaagcaacc gtgaccgtct tcatcccact cgccatcgcc gttgccgtct
     6481 ggtccgccta ccggaaccgc tacggtccag gacgctccgg ctggtggccg gacgcatttc
     6541 gaggcagtct cctcatctgc gtacccgcgc tgctgatctc tctcccctgg tacgtcttcg
     6601 tctacgccac ctacggcgac ctgaccgtct tccggtcgct cgatctgctc cagtcgtcat
     6661 gggggacgcc ggcagccagc ctgttcgacc tgctgctctc tccccatttc cagtccgagc
     6721 gcttccacga gggctggggc tatttcggct ggcggctgct gccgcttggc tcgttcgaac
     6781 tggccttcgc ctggatcgtc tccatcgtcg ctgtcgcggg gctagccgtc gaaggctggc
     6841 gttgctggcg ccggcgcgca tcgggagcga tcgaccctga cttggtcgcg gcactcgtga
     6901 tgctgctggc ctgctgcacg ctcttctacc tggcgatggt ctacttcggc acccgcgtcg
     6961 ccttcaccca gatgcgttac gtcttcccgg ccgcgccggc agctgccctg ctggccgcca
     7021 gcggctaccg cgcgctgttc gccaaccgct ggcgaccggc cggcgcaacc gccgtcgtcg
     7081 cgctggcgct gctcttccag atcctcatcc tgacccggat tgtgatgcct catgcgatgg
     7141 tctgatgacg atatccagcc gaacggtgtg ggtggggacc ggttatacat cgccatcgag
     7201 caggatgtcc gtgacgacga ggttcgtcgc gccgtcgatg actgtcacgg catctttgcg
     7261 gcggctggcg acgtaaatca ggttggtcgt tgggtcaacg gcaacggcga tcgggtcaag
     7321 catgaccggg atcgtcgtga tgccatggtt ggcgacggcg tcgatgaccg agacggtcga
     7381 gtagcgccgg ttggtgacgt agacgcgctg cgtgatcggg ttgatggccg cgtcgctcgg
     7441 gtcgccgccg accgtgatcg agaagtacgt gtcgaagttg gcggacggca gaccgacgcc
     7501 ttgtggatcg aatggcgcag gcaggatcgc gacccggcca gccgggccgt cgatgacatc
     7561 gacggtgccg ctgcccatca tcgcgacgta gacccggccg ttgcgtgggt cgacggcgtt
     7621 cccccaggcg tacgagccgc cgtcgatgtt ctcgatcaac tgattctcgt cagtgtcgat
     7681 gatcgagatc gtgccgctct cgcggttggt gacgtacccg cgcgccagaa ccgggtcgag
     7741 cgcgatcccg gccggatcgt cctggaccgg gatcgtcgcg acgacggcca gtgtcgcatc
     7801 gttgatgacc gagacactgc cgtcgcgccg gttggtcacg tagaccttac cggttcgctc
     7861 gttctgcgcg atcgcccacg ggtagtcgcc gaccgcaacc gttgcgacaa ccgccagcga
     7921 gctggtgtcg atcacgctga cgctggcatc caggcggttc gcgacgaaga cccgcccaac
     7981 tcgctcgtct acgacgatcc cgaccggctg gcggccgacc gggacggtcg caaagacctg
     8041 gcggctcgtg ctgtcgatga cggtgactgc atcagcgccg ctcatcgcga cgtagactcg
     8101 ctgtccggca ggatcgacgg caatgcccga cggcatcgcg gcctcgacct cggcccggcc
     8161 cggcagcggc agcgcgaacg cgatgacgac gatatcaatt gtgacgatgg cgacgatcag
     8221 ggcggtcttg acattcacgc agccggctcc aggtcgatct tggtcgcagg catcgctcgt
     8281 gcgcggtatc gttcccggcc tattcggaat cgtctcggcg atctaaagga cgacaatggc
     8341 gctccttgaa cagctagagc aggctggccc aaaccggcaa caagcgacag ggcaggacac
     8401 gctgcccagc gacgctgtgc aagcgccgcg ccactcctac tcgatcggcc tgcggacgcg
     8461 gatcgtgctg gcgctgatcg cgattgcctg cgtcaagcag gtcgttctgg ccgtcgccta
     8521 cccgcccttc gagggacacg acgaggtcgc tcacctcggt tatctggcga cgctggccga
     8581 cgacggccgg ctgccgacct tctccgacga cctcccggcc agcttggagg agtactcgcg
     8641 cttcacgctc gactggcctg ccgtctacac cgccaaccac ccgccgctct actacgtcgt
     8701 ctctgtcccg gcctacaaac tggccggcag cgaccatctc gcccaactct acgccgtccg
     8761 gctcgtctcg atcccgttct tcctgctgac ggtctggctc acgtacctga cagcgagaat
     8821 gctctttccg accgataacc tgattgtcct cggcgcgccg gcgatcgtcg ccttccagcc
     8881 acagctcggg ttcgagggcg cgatcgtcaa taacgacatg ctggcgattg tgttcgggac
     8941 gctgctcctc tacctctgcc tgcgcgcgat ccgcgacggc ctctcgatcc ggctggcgct
     9001 gataatcggg ctggtctgcg ggctggggct gctgacgaag gcgacactgc tggctctctt
     9061 gccgatcgtc gctgccgtct gtgtcttgtt gtgctggccg cgtccatgga cgcgcgttcg
     9121 cagccgcgac tggtggcagt cgacgctggt gcgcgcgctc gtcgtcgtcg gcccggccgt
     9181 cgcgctggcg ttgccttggt atctctacct gcgtcgcacc tacggagact tcactgcctt
     9241 cgaagcgacc caggagctgc aacgcaactg gaaccacccg gccgggacgc tgacggagct
     9301 gctcttctcc ccattgttcc acaaggaacg catccacgag acctggggct actacggctg
     9361 gcggctgatc ccgcttgatt caagagaact ggcggtcgtc tacgtgatga tgacgctggc
     9421 cggtttgggc gcgctgctgg ggctgctccg ctggttcggg cacgtgcgcg acaacggctg
     9481 gccgggtcgc accaccttcg ccggccagca ggcgaccagc atcacgacgc tcaccgccgc
     9541 gtcgctgctc atgtacggcg cgatggtcta tttcggaacg atgttcctgc tgacgcaggc
     9601 gcgctacttc ttcccggtcc ttccggtcgc gatcgtcctg gcgacggccg ggctgcgggt
     9661 gctggtccct gaaggctggt atcgagcggc aacgatcgtt ctggtcggcg gggccgtcgg
     9721 cttccaattc ctgatcctta cgaaactggt gctgccatat gcctacctct gatcgacacg
     9781 tcacgcgttc cgagcgcgcc gtgacggcag gactgaccgc catcgtcacg ctcgtcctgc
     9841 tggggctggc gctgctgatc ccgctcgata ctcgcaacgc gcgaggcgtc gagcagcaga
     9901 agaccgcctt cgacatctgg cggaggacac tggagcatgt cccggccgac gcgccgttct
     9961 tcctgacgac ggccacgctg acggtcctct cgctggccgc gctggtcgcc tcagcctggg
    10021 tgctggtcgc catcgcccgg ctgccgcgct agcgccgggt cagcatgagt cagaccggcc
    10081 ggcgccgctc catggcgacc actccatcgc tttcggcgaa gccgtcgccg aacgccggcg
    10141 cgcgtccgcg tacggatcag tcgaatcccg ccggccggct ccggggcatg ctgatctccc
    10201 gtcgctggga gatcgctctg ctggcgttga tcgccgtcgt cgcgatccag gttggtgcgc
    10261 tcgactggta cacctcctat cccgccttcg acgggtcggg cgacaagctg ccgaacacgt
    10321 tcgcctcgat cgaccacccg ttccacatca acaaggagca ggccgttctc aatgccctgc
    10381 gcgacggtca gttccccggc tggatctcca atcatcaagg cggctacccg accgagttct
    10441 acccgctcgg cgccgatctg gttgtcgcga ccggctgggc gttcagtctt ggccagcttc
    10501 cgctggaaat cgttcacaag ctggttgtta tcgccgtcct ctttatcccg gtgctcgcct
    10561 actgggcaat cgcccgccga gatcggttgc cgctcggcat agcggtcaca gccgggctgc
    10621 tgcacgtgtt cctgcccggc aattggctcg gtggcgggcc ggatgagcta ctgcggatgg
    10681 gcatgtggcc gaacgtcttc gccacatatc tgacgctgcc gctgatcctc tgggccgccg
    10741 gctaccttcg ccatggcagc cgtcgcagct tcgtgctggc cgccgtcgtc gcgtcactgg
    10801 cgatctactc gaacccgcgc tcaactatcg ccgtcgcgac ggccctcttg gcggtcggca
    10861 tcgtcgccct gctggaacgc cgcgagtctt gcccgcgtca gtcgcgaacc cggcagaaat
    10921 ggcgaactga catcgcgtcc atccggccgc tggtccgaag atcggtcctg ctggccctga
    10981 cgatcggact gctctgttcc gccttgctcg tgccacttcg cgctcaccag cacctctacg
    11041 agttctcgca cttcgtcgag ttcgatcatc ccggtcagat ctggagctac taccggcaag
    11101 cggttccgac cgagatcatc gtcctcgcgc tgctggggat ggtcatcggg cttgcccggc
    11161 gcgatttcta cacgcgggta ctggccgtct ggctgccgct ctcgtacacc gtcatcctga
    11221 ttgccggctg gctgttgcgt gacctctcca tcttcgccca gctcgaaggc ccccggctga
    11281 ttccgctgct gcgcctgccg tccgttttcc tggccgccgt tgcgctccgt gaggtcgttc
    11341 gcttcgtcat tcgcctgctg cgcgccgacc gtctgctacc ggccaccaac atcgccgccg
    11401 tcacgatcgt tgcgctggcc gtcctgacac ccgtctcgat gctcgaagac gaccagcgcg
    11461 gcctcccgga cctggagacg accgaccagc cggacttcgc tgcgatcgcg cgctcggcca
    11521 ggatctatca gcaagcgacg acgcccgacg atcgcccgtt cgtcatcgga tcgacgatct
    11581 ccgagcacgc tgcgttctgg attccagtcc tgaccggcga cgacgcgttc ctctccaact
    11641 gggtctggaa ctggcgcata ccggactacg ccaaccggac ccagatcgcc gacgtggctt
    11701 ctgcgctccg gctggacttc ctccgacgac atgccctgac gatggtgctg atctcaactg
    11761 acagcgacgc gacgctgaac gtcgcccgcg ccagcccgta tctggatgaa ctcgaccccg
    11821 gcacggcagg cggctacgcc atcttccgcg tcgacgatcc cggcccgaag acgcgtggcc
    11881 ccgtcacggc cggcgccggc acggtgaccg ctctcgacca gtcccgcgag cacctgcgcg
    11941 ccaccatcga aacaacgcag ccgaccgagg tcacgatcgc gatcaacgcc ttccccgcct
    12001 ggcatgcaac ggtcaacggc caagccaccc ggctcaaccg caccgccgac ggctccatgc
    12061 acctgaccgt tcccgccgga accagcgccc tggaactgcg ctacactacc gaaccggccg
    12121 tctgggccgg ccgcatcctc gtcacgctcg gcatcctgct gatgatcgct gtcgtgcttg
    12181 caccccgcct ccctaaaccg ccgcgtctcg catcacgcgt ctcgcgtcac aacccatagt
    12241 tcctctcgtg tacgatacgt ctccggaagg ggaggcacgg gagacgacga tgactgagga
    12301 ccagggacca cacgcgcgcc ggatcgcgct ctcgttcgat gtcggaggca cctttaccga
    12361 cttcgtcgcg atagacgttg cggcaggaga ggtagccggc cggcacaagg tcctgaccaa
    12421 tgcccgctac ccagcgcgtg gtgtcgttcg cggctggcag gagatggtcg aggcggggct
    12481 gacggcggag gctatccagc tctcggtgca ctcgacgacg ctcgtcacca acgcgctgat
    12541 cgagcgcaag ggcgcgaaga ccatcctgct ggcgacggca ggcttccgcg acctgctgga
    12601 actggcccgc gagcagatgt acgacatcta cgatcttttc gcgcctccgc ccgaaccgct
    12661 catcccgcgt ccgttgcgcc tggagatcga cgaacgcacc gatgccgacg gtcagatcct
    12721 gcgcgaacca tcgccggagt ccatcgaagc cgcgatcgcg acgatccgtg aatcgggagc
    12781 gacagcggtc gcgatcgcct tcctgcacgc ctaccgcaac ccggccaacg agcaggccgt
    12841 cgccgccgcg atccagcagg cgttgccgga tgttgccatc tcactctcat cggccgtcgc
    12901 gccgattgtc ggcgagtacg agcgtacctc gacgacggcc gccgatgcct tcgccaagcc
    12961 gtccgtccgc gcttacgtgg gcgagctgga cgactcgctg gcggccggcg gcattcgcgc
    13021 gccgctctac ctgatgctct cctccggcga gatcgcgtcg gcggcaacgg ccattgagcg
    13081 gccgatccgc ctgctggaat ccggcccggc tgccgggggg atcgcggctg ccttcttcgg
    13141 ccggctgtcc ggtcacgaca acgtcctctc gctcgacatg ggcgggacaa ccgcgaaggc
    13201 ctgcgtcatc gaggccggca agccggagct ggcccacatg ctggaggtcg ctcgcgttcg
    13261 ccgcttcatg gccggttccg gcctgccggt cgtcgcgccg gtcgttgatc tgatcgagat
    13321 cggcgccggt ggcggcagca tcgcccgccg cgacgagttg gggctcctca aagtcggccc
    13381 tgactcgtcc ggcgccgacc ccggcccggc ctgctacggt ctcggcggcg agttgccgac
//
LOCUS       DASUGJ010000614         4375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1629605, whole genome shotgun sequence.
ACCESSION   DASUGJ010000614 DASUGJ010000000
VERSION     DASUGJ010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629605"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VFV93_14025"
     CDS             <1..750
                     /locus_tag="VFV93_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX5166516.1"
                     /translation="PVAHLRRSLGLTPERKTNAYWRYLVLASTAPSLVMEHDYVSPTT
                     HFIRPEPFDRSGDETLPGWLTSLEGRPTIHATLGTVVSRLRVASTIYDAIVAAVRDEP
                     INVILTVGSNQEPDRFNPGEGHVYVDRYIPHSLLLPHCDLVIAHGGFSTMISSLCHGL
                     PLVVIPVAADQFDNADRVQRAGAGIVIDPDQRTPEAIRAAVRQVLTEPHFRKNAERIR
                     AELESLPNFDHAVSLLERLAVEKQPILAVTR"
     gene            1366..2037
                     /locus_tag="VFV93_14030"
     CDS             1366..2037
                     /locus_tag="VFV93_14030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5166517.1"
                     /translation="MATFVLVHGGTGGGWTWRDVARSLRAAGHEVYTPTLTGMGERVH
                     LAHPDIDLETHILDVANVLIYEQLTEVVLVGASYAGIVITGVADRVPERVRLLVFVDA
                     LIPEDGKSAVDIAGPEVAAIVEARSKQEGGWRVPAEPPLTGQTIRSLTTPIRLTNPAA
                     EDIERVYIYCTEKENPPPNIAVMARMAVKAREAGWPYYELATGHSPVRSAPDALACLL
                     LEIGG"
     gene            complement(2086..3558)
                     /locus_tag="VFV93_14035"
     CDS             complement(2086..3558)
                     /locus_tag="VFV93_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166518.1"
                     /translation="MTDESVTPTDKSKDDDAKVVLKDEPPIVTRHEMTLGGRTLAYTA
                     TTGMMPLKDDTGEIEAQIFFVAYTLDGPEGGPERPLTFAFNGGPGSASIWLHMGALGP
                     KRVALNDDGTMPPPPFRLVDNQETWLAETDLVFIDPVGTGFSRAAKDELNAKYWNYSG
                     DIESVGEFIRLYLTRYGRWTAPLYLAGESYGTIRASGLSGLLVDKGIVCSGVILISAL
                     LSYGTGASGNYGGHDLPYNLFLPSFSATAWYHKQLPDDLQSRELRDVVAEVEAWALTD
                     YLTALAKGDELTAGEKQAVTAQLARYTGLSEDYVDGSRLRIHIHRFVKELLRHRRLTV
                     GRLDSRFTGIDELAVTEGSDFDPSLTSPTAPFTTTFNNYVRKTLGYETDTEYETLSFK
                     VFKGWKDESAGRGFTETTGALRKAFARNPYMRVLVNYGYYDLATPYFAIRYSVAHMGL
                     EPDWRANVTFAGYESGHMMYMDRPSREKFWDDVRGFLTKG"
     gene            complement(3661..>4375)
                     /locus_tag="VFV93_14040"
     CDS             complement(3661..>4375)
                     /locus_tag="VFV93_14040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5166519.1"
                     /translation="VGEGYALIVNDLDPFRCEGVAGALSADGATVMAMTGDVSVREQA
                     EELVEMATGGWGRLDVLVNCAGIFPNTPVIEMDDAEWDRVYGTNLRGPFLLSRAAARA
                     MIAGNSGEARHIVNISSTAGESARTGAAHYCGSKAALNMLTKVLAIELAPHGIRVNSV
                     APGIIVDDVYERASPPADPYVAALLAGIPLGRTGRGDDIAGAVRFLLTPAAEWITGEI
                     IHVNGGSTAGRVTLPPSQF"
BASE COUNT          770 a         1432 c         1380 g          793 t
ORIGIN      
        1 ccggtcgccc atctgcggcg ctcgctcgga ctcacccctg agcgaaagac gaacgcgtat
       61 tggcgctatc ttgtcctcgc gtcaactgct ccgtcgctgg tcatggagca cgactacgta
      121 tccccgacaa cccacttcat cagaccggag ccgttcgacc ggtcaggtga cgagacgctg
      181 cccggctggt tgacgagtct cgagggtcgt ccgacgattc atgcgacgct cggcaccgtc
      241 gtaagtcggc tgcgagttgc atcgaccatt tacgacgcaa tcgtcgctgc cgtcagagac
      301 gaacccatca acgtcatctt gacggtcggt tccaaccagg aacctgatcg ctttaaccct
      361 ggcgagggac acgtatacgt cgaccgctac attccgcact cgttgctact tccacattgc
      421 gatctcgtca tcgcccacgg tggtttcagc acgatgatct cgtccctctg tcacgggcta
      481 ccactcgtcg tcattccggt cgccgcagac cagttcgaca atgccgaccg cgtgcaacgg
      541 gcaggcgcag ggatcgtcat cgacccggac cagcgcacgc cggaagcgat ccgcgccgcc
      601 gtccggcagg tgctaacaga accgcacttc cggaagaacg ccgagcgcat ccgcgctgaa
      661 ctcgagtcac tgcccaactt cgaccatgcc gtctcgctgc tggagaggct ggcggtcgag
      721 aagcaaccaa tcctcgccgt aacgcgttga gcggcgtggc cgatatccaa tggcataaca
      781 ccgggtgatc gcacgacctg gtgatcagac gcaaaccaca accagcactt ttatgatccg
      841 ttcgagttag ctcgatatcc gtttcgagtc ggaatggcca gagtacgagg ctgcgctccg
      901 aatggtcaga ttccagcccg gttggaaagc cttcgacgca gactgcgtct gcgggcgctt
      961 accgccgctg ctggctgtgg cagttgggca cgaggaccgg ccaaaatcga tggatcatgg
     1021 ccacgagact ggcgatacgg tccttgtact gcacgttctg aaccaactcg acccagcaac
     1081 ggtatcagcc gcgacacgtg tcttctggga gtagcaggcc gatccagacg ctcccagctg
     1141 taacgccaat caccccaact tcgcctttgc catccaatga gtcgtcactg tcaaccgagt
     1201 ttctgtcagg ggaagcggct aaaggctcaa cggagagtat tacctggaca aacacccacg
     1261 ggctggtagt cggcagagtc gtgtaacaat ggggtagcca cgatgacctc ggtctgatgc
     1321 ggcaactcgg gctgcttcaa tcctgaattg gtgcgaggag gatcgatggc gacgttcgtg
     1381 ctggtacacg gcgggacggg gggtggctgg acgtggcggg acgtcgcgag gtcgctccgg
     1441 gcagctggcc atgaggttta cacgccgacg ctgaccggca tgggcgagcg cgttcacctg
     1501 gcccatcccg acatcgacct cgaaacacac atcctcgacg tggcgaacgt cctgatctac
     1561 gagcaactga cggaagttgt cctggtggga gccagctacg ccggcatcgt gatcacaggc
     1621 gtcgctgaca gggtaccgga gcgcgtccgc ttactcgtct ttgttgatgc actgatcccg
     1681 gaggacggca agtcagcggt cgatatcgcc gggccggaag tcgccgcgat agtagaggca
     1741 cgctcgaagc aggagggcgg atggcgcgtt ccggccgaac cgccgctcac cggccagacg
     1801 atcaggagcc tgacgacgcc gatccggcta acgaatcccg ccgccgagga catcgaacgc
     1861 gtctacatct actgcaccga aaaggagaac ccaccgccca acatcgccgt catggctcgg
     1921 atggccgtga aggcacgcga ggcaggctgg ccctactacg aattggcaac tggccacagc
     1981 cccgtccgct ccgcgccgga tgcacttgcc tgcctgctgc tggagatcgg gggatgaggg
     2041 atggagcatg tgatgggttt cttgtcatga cccatccctc atgcctcatc ctttcgtcag
     2101 aaagcctcgc acgtcatccc agaatttctc gcgtgacggg cgatccatat acatcatatg
     2161 gccggattcg tagccggcga aggtgacgtt ggcgcgccag tctggctcga ggcccatgtg
     2221 agcgacgctg tagcggatcg cgaagtaggg cgtcgccagg tcgtagtagc cgtagttgac
     2281 cagcacgcgc atgtacgggt tgcgcgcgaa cgccttgcgc agcgcgccgg tcgtctcggt
     2341 gaagccccgg ccggcgctct cgtccttcca gcccttgaag accttgaagc tgagcgtctc
     2401 gtactcggtg tcggtctcgt agccgagcgt cttgcggacg tagttgttga aggtcgtcgt
     2461 gaacggcgcg gtcgggctgg tcaggctcgg gtcgaagtcc gagccttccg tgacagccag
     2521 ttcgtcgatg ccggtgaagc ggctgtcgag ccggccaacc gtcagccgcc ggtgacgcaa
     2581 cagctccttg acgaagcggt ggatgtggat ccgcaaccgg ctgccatcga cgtagtcctc
     2641 ggatagcccg gtgtagcgcg ccagttgcgc cgtcaccgcc tgcttctcgc cggcggtcaa
     2701 ctcgtcgccc ttcgccagcg ccgtcaggta gtccgtgagc gcccacgcct cgacctcggc
     2761 cacgacatcg cgtagctcgc gagactggag gtcgtcgggc agctgcttgt ggtaccaggc
     2821 cgtcgcggag aagctcggca ggaacagatt gtacggcagg tcgtgtcccc catagttgcc
     2881 ggacgccccg gttccgtagc tgagcagggc cgagatgagg atcacgccgc tgcagacgat
     2941 gcccttgtca accagcagcc ccgacagacc ggatgcacgg atcgttccgt agctctcgcc
     3001 ggccagatag agcggcgccg tccagcggcc gtagcgggtc aggtagaggc ggatgaactc
     3061 gccgaccgac tcgatgtcgc cggagtagtt ccagtacttc gcgttcagct cgtccttggc
     3121 ggctcgcgag aagccggtcc cgaccgggtc aatgaagacg aggtcggtct cggctagcca
     3181 cgtctcctgg ttgtccacca accggaaggg cggcggcggc atcgtaccgt cgtcgttgag
     3241 ggcgacccgc ttcggtccga gcgcgcccat gtggagccag atcgaggccg agccggggcc
     3301 gccattgaag gcgaaggtca gcggccgttc gggcccgccc tccggcccat ccagcgtgta
     3361 ggcgacgaag aagatctgcg cctcgatctc gcccgtgtcg tccttgagcg gcatcatccc
     3421 ggtcgtcgcc gtataggcca gcgtcctccc gccgagcgtc atctcgtgcc gcgtcacgat
     3481 cggcggctcg tccttcagga cgaccttcgc atcgtcgtcc ttgctcttgt cggttggagt
     3541 cacggattcg tctgtcatta cgtctccctg gtgtccggga ttcgggactc gggattcggg
     3601 ggtgacggag ggacgtttac cgtttgacgc ccccggttcc cgaatcccga atcccgcccg
     3661 tcaaaactgc gacggcggca aggtcactct tccggccgtc gagccgccgt tgacgtggat
     3721 gatctcgccg gtgatccatt cggcagcggg cgtcagcagg aagcggacgg cgccggcgat
     3781 atcgtcgcct cggccggtcc gccccagcgg gataccggcc agcagcgcgg cgacatacgg
     3841 gtcggccggc ggggaggctc gttcgtagac atcgtcgacg atgatcccgg gcgcgacgct
     3901 gttgacgcgg atgccgtgcg gggccagctc gatcgccagc accttcgtca gcatgttgag
     3961 cgctgccttc gagccgcagt agtgggccgc accggtccgc gccgactccc cggccgtcga
     4021 ggagatgttg acgatgtgcc gcgcttcgcc gctattgccg gcgatcatcg cccgtgccgc
     4081 cgcccgcgag agcaggaacg gcccgcgcag gttggtgccg tagacgcggt cccactcggc
     4141 gtcgtccatc tcgatcaccg gcgtgttggg gaagatcccg gcgcagttga ccagcacatc
     4201 cagccgcccc cagccgccgg tcgccatctc gaccagttcc tcggcctgct cgcggacgct
     4261 gacatcgccg gtcatcgcca tcaccgtcgc gccatccgcc gagagcgccc cggccactcc
     4321 ttcgcagcgg aacgggtcga ggtcattgac gatcagcgcg tacccctccc caacc
//
LOCUS       DASUGJ010000615         8853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1072644, whole genome shotgun sequence.
ACCESSION   DASUGJ010000615 DASUGJ010000000
VERSION     DASUGJ010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072644"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VFV93_14045"
     CDS             complement(<1..721)
                     /locus_tag="VFV93_14045"
                     /inference="COORDINATES: protein motif:HMM:NF015563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166520.1"
                     /translation="MSAPEVERGAVQDIADVTPLVVPNGPAEESSVPEVPTEGEKKPR
                     FTIPSAYTILFILIVLTAAATWIIPSGSYEYNESGEPIPGTYYSVDHEPQQILLDSLK
                     APINGLYGVQDDTGNISFWNYGELFGAIDVALFIIVIGGFLGVTMQTGAINAGIASVV
                     KKMQGREKWMIPVLMAICAAGGTSYGMAEETLAFYPLIIAVMIAAGYDALTGAAVVML
                     GAGIGVIGSTVNPFATGIASGF"
     gene            complement(754..1209)
                     /locus_tag="VFV93_14050"
     CDS             complement(754..1209)
                     /locus_tag="VFV93_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166521.1"
                     /translation="MKPWAIVVAIVAGLGIIALLVFDSDDRDFAQRANADTSTRPALQ
                     LAVLAGISDPERSISDVIENAELPPEVEETLTEVRDLLATAREQAQAQAQESLERASD
                     RLDSALQRVEDAADDTDNLARKGRLEQVHAALTAIQDQVEAWLDRLEAA"
     gene            complement(1224..2486)
                     /locus_tag="VFV93_14055"
     CDS             complement(1224..2486)
                     /locus_tag="VFV93_14055"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017764826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEX5166522.1"
                     /translation="MSAFGVHSEVGTLRKVMVHRPDLSLKRLTPSNHDDLLFDDVLWV
                     EQAQREHDDFVALMRGRGVEVYYFQELLAETLAASDEARGLLIEAVASPYTVGWSASD
                     EIRAFLWSLQPDKLAMHLIGGLTAAEANLDWVALREVSLIAAAIDDNDIFVLPPLPNT
                     LFTRDSSCWIYSGVSVNPMYWPARRREALNVFAIYRFHPMFQDAGFDWWYPERGESDR
                     FDVTDFGRATLEGGDVQPIGNGTVLIGMSERTQARMIEQIARSLFEKGAAERVIACVM
                     GKDRAHMHLDTIFTMLDRDTVTLYPKVAHEIRPISLRPGRKEGDFHVTVEKDFVSCLT
                     DALGVKKLTVVGTGGDKYQQEREQWDDGNNTVALGPGVVVTYERNTYTIANMRNAGVE
                     VLPIRGFELGKGRGGGHCMTCPLLRDPL"
     gene            2996..3697
                     /locus_tag="VFV93_14060"
     CDS             2996..3697
                     /locus_tag="VFV93_14060"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX5166523.1"
                     /translation="MSQTPVQVVIAAFQDENTAKQALDQLDRAQRDRIIHIQNAAVLR
                     RDHDGKLHVSETRDWSGKRGLAVGGLLGAAVGILAGPVGLAAGAGAAIGGLAARLRDS
                     GFSDDRLRRIGDGLLPGTSALVAVIEHTWVDQAERMLAEAGADVVAEAVRTDIAHELE
                     AGHEVVLTAVDTGDTLAAERLSMSDDRAEVTGTAVTADEATVYAAEVTSTTTGAGGAT
                     GQPTPSTPKTGDQAT"
     gene            3767..4621
                     /locus_tag="VFV93_14065"
     CDS             3767..4621
                     /locus_tag="VFV93_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166524.1"
                     /translation="MRRRVWLPLLIVAAVGMLLGNVASWLEINLVDSSRFVSTTTDVL
                     EQEDVRQAIADRIVERLLEEKPLVAAAAGDQIEAIVAGILGTDRFQALLTSIATQFHE
                     MLVTGDHPTITIESPVLQLLIVAVARVVAPEQAADLQIDDEPLVIELFARRDVPSLEQ
                     PIRVLRWTGLIASVIALGAMAIVILRSDDRHSAVRRCGITVVVAGVLLLVVVPAVRIW
                     MVGKVENASQETIVREFITAFTVRLAVQTGLLVLIGWVMIAYGYRLFAKLSAPAPDAV
                     PSPIVEHP"
     gene            5111..7879
                     /locus_tag="VFV93_14070"
     CDS             5111..7879
                     /locus_tag="VFV93_14070"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5166525.1"
                     /translation="MDDSASGSQLPGHPAHRHELLSSRLRPHRLRGKRVDRPRLIERL
                     REAPDGSVTLVVAPAGYGKSTLLAQWAASSDVPVAWVSLTASENHPVELMAYLVAALR
                     AAGVQLPGLDRLGNDERSTDMTSLMSVLLADLRDVSSPVALVLDDYDAIDEHATNELV
                     RTLIDSGPAGLRLIIGSRAGPALPLERLRLDGRMHVIGVDELRFSVAETQDFCNLMNL
                     ADSGEDSVDLQARTGGWVAALQLAMISIGDGARSAADLLGRFDATNQYLHEFLLQEVL
                     GTQSSAMRSFMLQSALLDRFSVDLCRETLDLGEPAQDLLDRSIQTNLFVEPVEEAGGW
                     YQHHPLFREFLLRHGMTALGERERIAVYERASVWHEQYGLNHEAIDYALHAGNWPRAT
                     RLIVPVAARLSGREGMAAVVEWFKVLPAQLLVSDPELAAFAALTGIRTGVLDDVPRLL
                     TAAQELYEAAGDQLGLAHVSSLHAAVASIRGDGPMLIQHAHRTMTLLDLHERQSAPAD
                     GPHGSVMDPEHTRVVLRGLAQMHEGNGFYCLGKATEAAQAFDAVCEYSDVHGLPVLAA
                     GALNGLGHVRLMQGRLDDAIAAYEGAAADGRLNIVSRRIGLMYLADIYRERDQLSRAE
                     TMLEECSAIMNTSRLPIWHGHLLMQQARLAWSCGETQQALELAQHASRSARDAGIRWL
                     ERRSDAFAMRVRVADGDIGAAIKWVYDRHLSPDDPPTYEHLWEHLAYSRALIAHGEPG
                     DAVGLLTRAMHAAEVDGRNGEAIRVGVLLALAYLDLAQTDTAVDVLARSLRYGEHGGY
                     LRVFADEGMPMIHLLKLARERGITPTYCTRILDALEAPGGRSAPSNRDARAEDITDRD
                     IELLRMFAAGTATEAIADTLGVSTSTANWHVANLYRKLGVWTRVEALDQARALNLVPG
                     DDASASVP"
     gene            complement(7980..8456)
                     /locus_tag="VFV93_14075"
     CDS             complement(7980..8456)
                     /locus_tag="VFV93_14075"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX5166526.1"
                     /translation="MSTLTVMTFENMTDAGEALKNLQRVQKADQILIEDSAVVIKDEN
                     GKVEILDKRSRAVKVGAVTGGVLGGFLFFFAPVAGVAIGATSGGAIGKLLDTGVEPTF
                     VEQVSNDLQPGTSALFVVTERGNLEAALAALRSLHGSVYHTSLPSEVQQALEEALR"
BASE COUNT         1538 a         2784 c         2878 g         1653 t
ORIGIN      
        1 cgaatccaga ggcgatcccg gtcgcgaagg ggttgacggt cgagccgatg acgccgatgc
       61 ccgcgccgag catgacgacg gccgcgcccg tcagtgcgtc gtagccggca gcgatcatga
      121 ccgcgataat cagtggataa aaggcaagcg tctcctcggc catcccatag gatgtgccgc
      181 cggccgcaca gatcgccatc aggacgggga tcatccattt ctcgcgaccc tgcatcttct
      241 tgacgacgct cgcgatgccg gcgttgatcg cgccggtctg catcgtgacg ccgaggaagc
      301 caccaatgac gatgatgaag agtgcaacgt cgatcgcgcc gaacagttcg ccatagttcc
      361 agaagctgat gttgccggtg tcgtcctgca ccccgtagag gccgttgatc ggtgccttca
      421 gcgaatcgag caggatctgc tgcggttcgt gatcgacgct gtagtaggtt ccgggaatcg
      481 gctcgccgga ttcgttgtac tcatacgagc ctgacgggat gatccaggtc gccgccgccg
      541 tcaggacgat caggatgaac aggatggtgt aggcactcgg tatcgtgaag cgaggcttct
      601 tctcgccttc ggtcggtacc tcgggcacgc tggactcctc ggccggcccg ttgggcacga
      661 ccaacggcgt gacatcggcg atgtcctgca ctgccccgcg ttcgacttcg ggtgccgaca
      721 tggcaacctc ctcgtgcacg tgcgctcaac ccgtcatgcg gcttcgagtc ggtccagcca
      781 ggcctcaacc tggtcctgga tcgccgtcag cgcggcatgc acctgttcaa gtcgtccctt
      841 ccgggcgaga ttgtccgtgt cgtctgccgc gtcttcgacg cgctggagcg cactgtccag
      901 acggtcgctg gctcgttcga gcgactcctg cgcctgcgcc tgtgcctgct ccctggcggt
      961 cgcgagcaga tcgcgaacct cggtcagcgt ctcttcgacc tcaggcggca gttccgcgtt
     1021 ctcgatcaca tcggagatgg accgctcggg gtcggagatg ccggccagta ctgccagttg
     1081 cagcgccggc cgcgtgctcg tgtccgcatt cgcgcgctgc gcaaagtcgc gatcatcact
     1141 gtcgaagacg aggagggcga tgatgcccag accggcgacg atggcaacca cgatggccca
     1201 cggcttcatc gtcgatctcc tcgctagagt gggtctcgca gcagcgggca ggtcatgcag
     1261 tggccgccgc cgcgcccttt ccccaactcg aacccgcgta tcggcagcac ctcaacgccg
     1321 gcgttgcgca tgttggcgat cgtgtaagtg ttgcgttcgt aggtgacgac gacgcccggt
     1381 ccgagcgcga cggtgttgtt cccgtcgtcc cattgctcgc gttcctgctg gtacttgtcg
     1441 ccgccggtgc caacgacggt gagcttcttc acgccgagtg cgtccgtcag gcaggacacg
     1501 aagtctttct cgacggtcac atggaagtcg ccttccttgc ggcctggccg caggctgatc
     1561 gggcggatct catgcgcgac cttcggatag agggtgaccg tgtcgcgatc gagcatcgtg
     1621 aagatcgtgt ccaggtgcat gtgcgcgcgg tccttgccca tcacgcaggc gatgacacgc
     1681 tcggcggccc ccttctcaaa cagggacctg gcgatctgct cgatcatgcg tgcctgggtt
     1741 cgctcgctca tcccgatcag gacagtccca ttgccgatcg gctggacatc gccaccttcg
     1801 agcgtggcac ggccgaaatc ggtcacgtcg aaccggtcgc tctctccacg ttccgggtac
     1861 caccagtcga acccggcatc ctggaacatc gggtgaaagc ggtagatggc gaagacgttc
     1921 agtgcctcgc gccgccgtgc cggccagtac atcgggttga cggaaacgcc gctatagatc
     1981 cagcaggacg agtcacgcgt gaagagcgtg ttgggcaggg gcggcaggac gaagatgtcg
     2041 ttgtcatcaa tcgcggcggc gatcagcgag acctcacgca gcgccaccca gtcgagattc
     2101 gcttcggcgg cggtgagccc accgatcagg tgcatcgcca gcttgtccgg ctggaggctc
     2161 cagagaaagg cgcgaatctc atcggacgct gaccaaccga cggtgtaggg tgatgcgacc
     2221 gcttcgatca gcagcccgcg cgcctcatcg ctggcggcca acgtctccgc gaggagctcc
     2281 tggaagtagt acacctcgac gccacgcccg cgcatcaatg cgacgaagtc atcgtgctcg
     2341 cgctgggcct gttccaccca gagaacatcg tcgaagagca ggtcgtcgtg gttgctgggc
     2401 gtcaatcgct tgagactgag gtctggacga tggaccatga ccttgcgaag cgttccgacc
     2461 tcggagtgaa caccgaatgc tgacacgtca acacactcct cttgctcatg cgatctgaat
     2521 cgacgcaccg tcacagttcg tgatggcggc gacgcagggc aacgttggtt gccacatgtc
     2581 caccgtgtga cgcgcagcgc ccagccgtcg agtacctcat ttgatggtca gggtagtcgt
     2641 gcgctgatat ggctggtacg cccaacgagg tgtaaacggg gggcgtatgt cgctgtcgaa
     2701 tgtgagaccc cttcaatagt ggctgatggc tcattctgca cgccggtacg cccggccatg
     2761 gggcagcgta cccgcccgct cagctgtggg aaggcgcaac ttctgccgcc tggacgcttc
     2821 gagttgaagc gccatgcgcc agtcgggtgt agtcacaggt tggagtcgaa gtacacccag
     2881 cgtctacgtc cgagcgtccg atcacggctg cggatgcagc ggttagcgtg agagcggttc
     2941 ggcctgcaac gggctgagcc cgatcccggc tgtgtcagta cgaaaggacg ccaccatgag
     3001 tcagacacct gtccaggtcg tcatcgccgc gttccaggac gagaacacgg caaagcaggc
     3061 cctcgaccaa ctggatcgcg ctcagcgcga caggatcatc catatccaaa atgccgccgt
     3121 gctccgccgt gaccacgacg gcaagctcca tgtctcggaa acgcgtgact ggagcgggaa
     3181 gcgcggcctg gcagtcggcg ggctgctcgg tgcggcggtc ggcatcctgg ctgggccggt
     3241 cggcctcgcg gccggcgccg gagccgccat cggcgggctc gcagcccgtc tacgcgactc
     3301 cggattctcc gacgaccgcc ttcgccgaat cggcgatggt ctcctgcccg gcacctccgc
     3361 gctcgtcgcg gtgatcgagc acacctgggt cgatcaggcc gagcgcatgc tggcggaagc
     3421 cggcgccgat gtcgtcgctg aggcggtccg aacggatatc gcccacgaac tggaggcagg
     3481 acacgaggtc gtcctgacgg ccgtcgacac cggcgacaca ctcgcggccg aacggctctc
     3541 gatgtcagac gatcgggcgg aagtaacggg aacggccgtc acggccgatg aggccacggt
     3601 ctacgccgcc gaagtgacct ccaccaccac cggcgccggc ggcgctaccg gtcagcccac
     3661 gccatcaacg ccgaagaccg gtgatcaggc cacctgagcc cggaggatcg ccataagcca
     3721 ggttgacgcg gttcagagac cgcgctcgtg acgaaaggtc acgccaatga ggcgtcgagt
     3781 ctggctgcca cttctgatcg tcgctgctgt agggatgctc ctcggaaacg tcgcgtcatg
     3841 gcttgagatc aacctcgtcg acagctcccg gttcgtgtcg acgacgactg atgtcctcga
     3901 acaggaagac gtgcggcagg ccatcgccga ccggatcgtc gagcgactgt tggaggagaa
     3961 gccgctcgtg gcagcggccg ccggagacca gatcgaggcc atcgtcgccg gtatcctcgg
     4021 caccgaccgc ttccaggcgt tgctgacgtc gatcgcaacc cagttccatg agatgctggt
     4081 aacgggtgat cacccaacta tcacgatcga gtctcctgtc ctgcagttgc tgatcgtcgc
     4141 tgttgctcgt gtcgtggcgc ccgagcaggc agcggacctg cagattgatg acgaaccgct
     4201 cgtgatcgag ctcttcgctc gccgggatgt tccctcgctg gagcaaccca tccgtgtcct
     4261 gcgctggacc gggctgatcg caagcgtgat cgcgctgggc gcgatggcga tcgtcattct
     4321 ccgcagcgac gaccgccact cggctgtccg tcgctgcggg ataacggttg tcgtcgcggg
     4381 cgtcctgctg ctagtggtcg tgccggctgt ccgtatctgg atggtcggca aggtcgagaa
     4441 cgcatcgcag gagacgatcg tgcgcgagtt catcactgcg tttacggtgc gcctggccgt
     4501 gcagacgggc ctgctggtgc tgattggctg ggtgatgatc gcctatggct accggctgtt
     4561 cgcgaagcta tccgcgccgg cgcctgacgc ggtccccagc ccgatcgttg aacacccata
     4621 gctggaccaa tcgtgaccca gccgcgccgt caaccgtggt cggtcttcag ggagcgcgtg
     4681 tccccggctc ctgatttcag gggggcggag ccggggttgc gctccggcgg tgttggggca
     4741 ccgccggaag ggggtagaag ggaaggggaa gatagagccc tcgcgagacg accggagaga
     4801 gtttgcgaag cagacgttga gcgacggagc ctgttccagt accggatcgg ttccgaaagc
     4861 agccgtcgtc cgaatgcttc gctcgcggac cgtctctgcc agtacaacat accaagcgcc
     4921 ggatgctggt cgaatcaacc gaaccggtgt acttgcaggt cgtaggattc cggcatatcc
     4981 gctacgtcga atatccgaca caactgtcgc gaaaatgcgg catcgcgagg ctccgatgga
     5041 atagaatctg aacgtcagtg ttgtgagtac cactgcgacg agcggcgcgt gtccaggatg
     5101 gggccattcc atggacgatt ccgcatcggg ctctcaattg ccggggcatc ctgcccaccg
     5161 gcatgaactc ctgtcatcgc gtttgcgacc gcatcgcctg cgcggcaagc gtgtggatcg
     5221 gccgcgactc atcgaacgcc tgcgcgaggc gcctgacggc tcagtgacac tcgtcgtggc
     5281 gccggcgggg tacgggaagt cgaccctgct cgcgcaatgg gcagcttcga gcgatgtgcc
     5341 ggtcgcctgg gtatcgctta cggcctccga gaaccaccct gtcgagttga tggcgtacct
     5401 ggtggcagca ctgcgcgccg ccggcgtgca gctacctggt ctggatcggc tgggcaacga
     5461 cgaacgttcg acggacatga catcgttgat gtcggtgttg ctggcagacc tccgcgacgt
     5521 atcgtcgccg gtcgcgctgg tgctggacga ctacgacgcg atcgacgagc acgcaaccaa
     5581 cgaactggtc cggacgctaa ttgacagtgg gcccgctggc ctgcgcctca tcattggcag
     5641 cagagccggg ccggccttgc cactcgagcg tttgcggctc gatggacgta tgcacgttat
     5701 cggggtcgat gaactgcgct tctcagtggc tgagacgcag gacttctgca acctgatgaa
     5761 cctcgccgat tcgggtgagg acagcgttga cctccaggct cgcactggcg gctgggtggc
     5821 cgctttgcag ctggccatga tctcaatcgg tgacggcgcg agatctgcgg ccgacttgct
     5881 cgggcgcttc gacgcaacga atcagtactt gcatgaattc ctgctccagg aagtgctggg
     5941 tacgcagtcg tccgcgatgc gctccttcat gctccagagc gcgctgctcg atcggttcag
     6001 cgtcgatctc tgccgcgaga cgttggatct cggcgagccg gcgcaagacc tgctcgatcg
     6061 ttcgatccag acgaacctgt ttgtggagcc ggtcgaggag gccggcggtt ggtaccagca
     6121 tcatccgttg ttccgcgagt tcctgctgcg tcacgggatg actgcactcg gcgagcgcga
     6181 gcgcatcgcg gtgtacgagc gggcaagtgt ctggcacgag cagtatggtc tgaaccatga
     6241 ggcaatcgac tacgcgcttc acgcggggaa ctggccccga gcgaccaggc tgatcgtgcc
     6301 ggtcgccgcc cgcctgtccg gtcgcgaagg gatggctgcc gttgtcgagt ggttcaaggt
     6361 tctgccggcc caactcctgg tgtcggatcc cgaactcgct gccttcgcgg cactgaccgg
     6421 cattcgaact ggagtgctgg atgacgtacc gcgtttattg acggcagccc aggaactgta
     6481 cgaggcagcc ggcgatcaac tcggcctggc gcatgtttcc tcgctccacg ctgccgttgc
     6541 gagtattcgg ggtgatggtc cgatgctcat ccagcatgca caccggacga tgacgctcct
     6601 cgatctgcac gagcgccaat cagcgccagc tgacgggcca catggctcgg tgatggaccc
     6661 cgaacatacc cgcgtggttc ttcgcggact ggcgcaaatg catgagggca acgggttcta
     6721 ctgtctgggt aaggcgaccg aagccgctca ggcatttgac gcggtttgcg agtattcgga
     6781 cgtgcatgga ttgccggttc tcgcggccgg cgccctgaat gggctcggcc acgtacggct
     6841 gatgcagggg cgactcgacg acgctatcgc ggcgtatgag ggcgccgcgg ccgatggcag
     6901 gttgaacatc gtcagccggc gtatcgggct gatgtatctg gcagacatct atcgcgaacg
     6961 cgaccagctg agccgtgccg aaacgatgct ggaagagtgt agcgccatca tgaacaccag
     7021 ccggctcccg atctggcacg gccatctcct gatgcaacag gcgcggctgg catggtcgtg
     7081 tggcgagacg caacaggcgc ttgagctcgc tcaacacgca tcgcgctcgg cgcgcgatgc
     7141 gggcatccgc tggctcgaac gacgatccga tgcgttcgcc atgcgcgttc gtgttgcaga
     7201 tggtgacatc ggggcggcga ttaagtgggt atacgaccgt catctttccc ccgatgatcc
     7261 gcccacgtac gaacatctgt gggaacatct ggcctactca cgcgcactga tcgcccatgg
     7321 tgaaccgggg gatgcggttg gcctgctgac ccgtgccatg cacgctgcgg aagtggacgg
     7381 tcggaacgga gaagctatcc gagtcggcgt gttactggca ctcgcgtatc tcgatctcgc
     7441 acagactgac actgccgtcg atgtgctcgc tcgctcgctg cgctatggag aacacggcgg
     7501 ctacctgcgc gtctttgccg atgaaggcat gccgatgatt catctgctga agctggcacg
     7561 cgagcgcggt atcactccca catactgcac gcgcattctc gacgcgctgg aagcgccggg
     7621 agggcgatca gcaccgtcga atcgcgatgc acgggcagaa gacatcaccg accgcgacat
     7681 cgagttgctg aggatgttcg ccgccgggac cgctaccgaa gcgatcgctg acacgctggg
     7741 agtctccacc tcgacggcga attggcatgt cgccaacctg taccgcaagc tgggtgtctg
     7801 gacgcgcgtc gaagcactcg accaggcacg ggcgctgaac ctggtgccgg gggatgacgc
     7861 gagcgcgtct gtcccttagg cccacccgat tcgatctccc agcacgccat gcgacacgtc
     7921 catcctgacg aagacgcgat gcaggtcgcg tgaatcgccg gcaacctcgg catggacgct
     7981 tagcggagtg cctcctccag cgcttgctgg acctcgctcg gcaggctggt gtgatagacc
     8041 gacccgtgca acgaacggag agcggccagc gcggcttcaa ggtttccacg ttcggtgacg
     8101 acgaagagcg ccgatgtccc cggctgcaga tcgttgctga cctgctcgac gaaggtgggt
     8161 tcaacaccgg tgtcgaggag cttgccgatc gcgccaccgc tggtggctcc aatcgccaca
     8221 cccgcgaccg gcgcgaaaaa gaacaggaag ccgcccagca cgccaccagt cacggcgccg
     8281 actttgactg cgcggctgcg cttatccagg atctccacct tgccgttctc atccttgatg
     8341 acgaccgcgc tgtcctcgat caggatctgg tcggccttct ggacgcgctg caggttcttc
     8401 aacgcttcgc cggcgtccgt catgttctcg aaggtcatca cggtgagcgt actcatcggc
     8461 atcacctctg ctctggaaca catgcggttc gggaggtggc gttcctgccg gatgaacgtc
     8521 gcgggtgagc gccggcgtct agtgccggcg tgttgtgcag cgcgtctctc agacaggcat
     8581 cgccgctttc aggttacgag gtgtgctggc cgttctggta gcaccccgac ggtgtatccg
     8641 agaggcgtag ttatcgtgac gtagcgcggc gcctgtgtcg tcgttgtcga attacggctc
     8701 ggacgggacc gattcggccg caaccgtgtc cgtatccgcc agcggtggcg cccgctgcgc
     8761 ttccagggcg gcggttccgt cgccggtgct gaccagcagc gccagccagc gggcattggg
     8821 gctccgttcg agcagcagga tcttgaaggc gac
//
LOCUS       DASUGJ010000616        22470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_655258, whole genome shotgun sequence.
ACCESSION   DASUGJ010000616 DASUGJ010000000
VERSION     DASUGJ010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 22470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22470
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655258"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            741..16973
                     /locus_tag="VFV93_14080"
     CDS             741..16973
                     /locus_tag="VFV93_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEPR-XLL domain-containing protein"
                     /protein_id="HEX5166527.1"
                     /translation="MSSISPKFIPHSEKAASGLLTRLLSRLWTSAPERGPTSRRARFE
                     ALEPRILLSADLTPETADALADGLNRLGNRVEDFLVSETLLDQRIPLLLSVKENDDGI
                     VVDEAPTISELYVVPVDANGDGDIDDFIDPFDDDESILQDLDTAGNADGSVDAGEFLQ
                     GWFFDPLEAFLNGHPATTTDVVDFLEGGFLFSLDQSLSIGPYTIEFEIVDAKVADLTE
                     DPDAEAAFSVGFVLSITQDLPIDLGLEADALKLLPFTGPDASPEPPVVETTSSLSFGF
                     TFGVFTGGQTPAQITGSDFFIRKADPLLVSVTAEDTDLDFKLNVGFLGAEVVDGSLDL
                     QVDVETTLLDPNDPDVLGFIDTQHGVESTTGVVTATNAVPSANLDHAAGFFLRVGNVG
                     ITTPVLVLDNAAADLNALKTHVESALAAANLDDLIDVSLVGAGNDTFQFSLIPTSDTP
                     LGFANEALGLLGSLSGTPDGDGPNAFEYLSDQVFLLSVGGGLARIITVRFPDPAKEEI
                     GFGHEQTADLTAAADQVVAANTPTVFDITGNANFTVTITFDNGTQLSQSFTVTATDTD
                     GSGNLAQLAGAIDAQLVADTTIFPFVQVLDEGGKIKFEGINSSVSGIRVQASGTATSE
                     IGFASDADATLSLTAANNAINTGDLSGTATFDVTVTTVGVGTSTTRVTVAPSDNGGVA
                     DLAADIDAALASQGLAIDASVVSGKIVLTAQNVNVAAFDVKVGNQDLDDLVADVNAAL
                     DDAGLGSAVDASNDGGGHLVLTSTAGKSLEISRTLTFDAGVTFTELGLGANQLFSTTT
                     GADSHVTFDFPVQVLAGLEDATTEADDDWDPADVAIVGNFDPFGPLSVFDAGGENRFN
                     LNLTVDPPSAADAPIAVPTPGSLTDEIKLVNFAELLQFNVVAAESMIGLITQLGQSLE
                     SIASSGQFSLYGIPLSGSALADMLVFGDLVQTGLVYDVGEDGQLDATASKPNNHADLV
                     ADGEVSRLQVRTIVHADGQDTVKLLPSFVTAQQLASQLSTLLGIPLGPNGINARYDPA
                     DGSTTGNELSYTITLVSKDRISVGVEDSVGEADAFHAPFEFDVNLSPFGELTVDTVDD
                     MGNPLDSRVTLTSRNSLQMTFGVDLDPAAPIDFADGGTDVQDLNNETGVDIKLERAVT
                     GTDPVRQVFGIGSDATFLVSIDDGAPVQVTLSKAVVDGTDGSLNAFADELEEVINDAL
                     AGDATVSVTVVPNEDGKAEDRLRITLTAGGTTIDLSPVSSDPSALVELGFDSQAPADT
                     DGIFTAINSAPSLYALTGNATFSVQVGANPTVSVTILASATAGNQSISELVADVNNAL
                     RAAGLLVLVGDEEQGIRAAFDAGRLVLTATAAATQFSVTVNGATAAGTVAHEELGLAL
                     GTKVADKDDFIVIDRNGIEHRVSLDTAADGDVQDLIAVINTAGTGFFEAGANASGTGL
                     RLTDITTPPMGVDNRPAIRVATVNGSLAALKLGWLTNPTNPDDIDNENAGDPINPDFI
                     EGGPIGTRALPERLFARDGELSGAFSMDLPGGGIAGDALVGIVGVDVTMSGGLTAELS
                     ANLQAPGGTPGSKVTLAQLQANAGAVPSNQQIVTDPLVSKISTLNYDTEVSSFSAGRI
                     VRGATSGATAVVLEVTDNGSTGTLTLFDIDGTFQDNEAIVDTVNALNGAALANGGVTQ
                     QAYFGEFELDFAVKDGFADTGFGDDLEAALDGLTGTVPVRLTGFGNVNTHVVPATFTA
                     PVVELVDVPGNLGALYDFVNLGYGDLTAALEQLEPLVHALEDDFAVLHQKLPIVNRSV
                     NDLIRIADGFSLSVDNFAEVADDASALLHPDQEDVPTLTLQGVAQALRGAFGLGSNST
                     GIVVDVTSDGLLTVEFDLLETVSTTLGLDVDLSSGGLPNLTSGKVLQVSGELGLAATI
                     GIDLGDPSNVYLFEENAGLSGNLSVLGNGAEDLGLVFLAMVGDVPVLVRDGLVDMDLE
                     FTLGDLDFSGESTAGRKSLPAVQFGDFTAAVPVESFIASVPLFDPDGGLTGFIGEIAA
                     DGDLGGLSLPDIDSLADQLDEIASSLPEFNPLDNLLLLAENIDFFLENLQDFFDLELF
                     GIKLPIVGDDFADGARFIEEFRTSFVSDLRATIESATDIQLGDITDVIEDFFAGVGEG
                     GIDVSPISVESDLDNEDFGERYAQWNFELSDTLIVPFDLEFDIGVPLLGMDIDIPLII
                     SIGWSLEWGFGVSFEEGAFIYTGNDEELSVTVDIDLPASESFSGNLAFLELIVANAGS
                     GVHLEFDVDLENGNGGDRLGISDLGALELTPFVEGAAIHDHILDLDLTVGIVGVATAF
                     PNITANFILDWELPRTALGDLTGDALASGLTKIALNDIRLDAGSFLTDFLGSTLGEIR
                     KFTEPFDEIIDVLTTPIPVLSDLAGEDVTLLDIAGVFGEVDVGFIEWIGTVLDIIDTI
                     GSLDDDTQLGIDIGSLTIYDSVGGGAIGSDFKGDLFDEDMNLADRIDLGTDANPIAAL
                     TNFDPNFNNRLNAADAGEGSGDAADAKSALQDLREGNLAEIISFPFLDNPLSIVGLLL
                     GEDAILIQMDAPPLLVSFDYTQVFPIFGPLAATVGLGFKVGVDFSFGFDTFGVRRFAD
                     SGFTNPALIFDGFFLGDRENVTEGADINELFFEFSLTVTAELNLAIARAGAGVQGTAF
                     IGFDLFDPDNDGAIHLSELINTIENQLRSDDPGLAPLSIFDLTGKLTLELFAFLIIDF
                     GIFEIDYEFPITPEIEVFTFDVSFPRPPILATDLDTGTLQLNMGPNAVDRLNGDVRDG
                     SEEFHVRKTADGIDVWSPSLGVGDGSPGDGTQHYTGDFDHIVALGGQGNDQIHFYDFD
                     ESDITFDLDGGVGNDIIHFHESAAVAPAAGAGARIRGGVGDDVLIGSHLDDEIHGGEG
                     NDRIEGGRGYDILFGDDGTLGVLTNGDRFVGKRISVKDGDDEVYGGSSTDQDLADADA
                     DDIIVGGGGEDELHGDGGSDVILGDGGRILYALTDGHIHVASLRPDPFVPNDKADPSP
                     AVSTSQKIENIFLQLSSHFSGTDLGDGADDEIHGGAGGDILFGGSADDEIHGDGGDDY
                     VVGGMGFDSIFGGDNDDTLFGNAQNDYMEGNDGSDVMSGDFGDDVMHGNAGDDYMRGG
                     RGLDVMYGDEDDDEVLGEGEPDVLFGGIGNDLVIGSVGADIMFGDDGIVAKLDTDDVG
                     PFNDVSVGIGNPALITAAMRAAADNLAGSLDLILTDAVASDGNDILSGDGGGDIILGG
                     GGDDLIGGDVDPRLAIANTPEKTTEAGEDFLIGDGGIVQFDRRRLQYVASVVDNDPTS
                     FRDTIYGDNAPDVIIGGQGGDSGSFMLGTREITRMLAGGHGPGRGETDPEVGQNDIII
                     GDNGELFYANDDIAENFGRLEQVRTTDTSNSTGGADMAHGELGDDIIFGGVNGSVDVL
                     FGNVGNDVMLGDNGEVDFAFGQDTASPLDDDTNLDTLDLIRSYRDGLGGTDEISGNAG
                     DDVLMGGTGGDLMYGDDAMASAGTDDGEDVMLGDNADIFLIGTTGRLKVKVAAMTTGT
                     AVDLITTSDRIDPLDPNHDTVAEVEDDGGADTMSGNAKADIMLGGVNNDNGLGDPEVD
                     TMYGDRAAPTAASIVLDSDDILLGDNGLLDFTFGDSDQNILDLIRSFEDGLGRTDVIS
                     GNKGLDVAIGGSFDDHIYGDDTAASAAAADLGDLLLGDNADVFLVNPNGASGGDTKLV
                     LDAAVKTIRTTDEEHPEYGGSDTISGNAKGDIIAGGVQGDWLYGDAASIQPALDGDDV
                     ILGDNGAFEWLSTGRLGEIEGISIALHNPLLFAKFNGDVADTDLTTLDLITTEQPTSG
                     GRDTIHGDEGDDLIFGGTDLDRIRGDDGDEAAETLSANRDVLFGDHGRLYPHFSALQD
                     FNSRNFFAIDTGDSDGGEGDVMYGEEGDDVMLGQQGDDRMWGGTHADDMIGGHNVQGG
                     IDELSVPAVQAVSNPGPANSNDLMDGGSGNDAMKGDNVIVWRRGDDLSPRFQALVGTR
                     LYTTGPDGVDTIVTNVSGVAQSDPSDTVGRDVTVVDHADVVPAGVFGDDLMAGGADDD
                     FMLGDLGNDVMQGDGSISTVADPGPNTITTTHFDGGLPDSSGTLYFNVPEAASDGDDY
                     IEGSGGDDLLYGGLGQDDIIGGSSSLFGLDTEEERPDGSDIIFGGAGTPARLARNDFV
                     GATDTDTGTGEGVGAVPTGDDPQIALADRHSRDADFIMGDNANIYRIIGPGDAYRQFN
                     YDQSSVYENRGNERIVVRAMQQLDYTLGGADYAGGSYAGGVANVDNGLADLIHGESGD
                     DYIFGMTGSDVIFGESDDDDIVGGYGNDWISGGTGQDGVLGDDGLLYTSRNSTLGELL
                     YGVAGLLSSDAMTKYNNGNALNEIISTPGDVQYAVINVTGELKKTADLVPFSFDAEWL
                     GLDDEFPDDASNTPFADDIIFGGLGGDFLHGGSGDDAISGAEALEHAYVPTFDVNGLP
                     NGVLDLGYDAFDLPAPVQPGDFVPNVNPGNVLHFNPEDLDGQHLNNRFRAGEFYLYDE
                     YDPLRRIELTSSGELWKPGDGASFAFLLNFDQTEGIFRASGTVPKATGQQTESYPAVS
                     DDGKDAIFGDLGNDWLVGGTGRDNVYGGWGNDLLNADDDLSTLGDAPKQGDPAVEGAN
                     DVPDTHPFYEDRAYGGAGRDVLIANTGGDRLIDWVGEYNSYLVPFAPFGQATVSRTMM
                     PHLHEFLYALSAGDGADATRYADAIGGNAPAPTNNNPIPSRNGEPHGELGLVLQQDFA
                     WGDQTGAPSDPQAGNIPGGPRDVLRSAGFQDGSMEGFFVDSGVFTVQSGVLKVAAASL
                     GGDAVSVFHVGDALPGYFEVQASISAVKPTAGWKANSYVIFDYQGEDYFKFAGIDVSN
                     SKLVMGHRDASGWHMDEQASVQGGLKADKVYNVLLAINGVNATLVVDNKNIFSHTYQP
                     RVVDGYSYGLNNGMIGVGSDNSRGTFDNVRVLVLPPAITFQSTDDFENGVADLLPASG
                     QAGAWAVQDGRLTGSPVAGGIATSLVDLGIGGLDVSSYFELAATVNTQARAGFVFDRY
                     SATDFKFAAIDAVTDQVMIGHYTERGGWTVDAFVSKPINGGASYQLGVAIKGSTVSVT
                     LNGQAVLGTVFNAVAVDGDFGLLASGTPASFDGFIVKTDDAAFAQTSGSNMLAATAAP
                     GEIEAGATLTQSVLDSIATVAITQWTDALGNGDPRLASLGDVRITVADLAGDQLGYFD
                     GANILVDADAAGHGWFVDVSPADSAEFSIRLDRNVVAATGSSDAFARMDLLTVMLHEL
                     GHAISFTDNQPGYAVMDEDLQSGVRYLLNADTDSPMNDRALLQSASNGARPEARGGMP
                     GFDLDGGPAVAGGASIDWQAPSGDSWGVRYSPYASGKTSSNSSGNFSDFLFKIFKGSE
                     TSGSAGYDSLGNDLLGAKEGKGKPGAGNAR"
     gene            16998..17138
                     /locus_tag="VFV93_14085"
     CDS             16998..17138
                     /locus_tag="VFV93_14085"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX5166528.1"
                     /translation="MTIQLTDRITISPYASKRLTMLLDGVVKEYEKRFGALNVEEPTP
                     QR"
     gene            17379..>22470
                     /locus_tag="VFV93_14090"
     CDS             17379..>22470
                     /locus_tag="VFV93_14090"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5166529.1"
                     /translation="MSNPKTSSSNPSTRWPSLAEIFFGRLANLWRKRRMQRTFAGRRL
                     QFQAIEPRILLSADFFVDNPGDYLVTNDQGIIGLSNGDTVTWNPGAGSAHGGPVSALT
                     FGVNAFGRIQDAINAAAPGDTLRVAGGTFSELVTVNKSVEVLGNQFGVDARTRAVVPE
                     SIVNGADFGGGHSTSFNVTASNVEIDGFTVQQATSATQFGTGILLGAGISGSQIHNNI
                     IQNNITGLYLANGAGNQAVIEQNLFLTNNVAGPVSGTGIYTDQFVAGGTVSNVLIDNN
                     TFVGNTGQAIFFSSTNAAAPATNIVVSNNLFDVNGRALVAFNLATSQIVGNTMRNSTD
                     SATADIRLFEGVNDLSITGNVLQNGAGQAVRISNIGTGSPSATDVSFTLNSLSGYTGP
                     SGAFGVDAGGYTGILNATGNWWGSSTGPTTAANPGGTGLTLSDPFAVVDFQPWLLYGD
                     FNDSAVGFQIPATFTVAAQTDSFTATNNNYRRMVNAIESLQPGQTLLLSGVFDWTEAN
                     ASAAWALGNDGTSATVDDFTLTVRPNLNNAALTAASLGSARIQGPGDLAGENLEGFLV
                     FNGGDNQNWTISNLEIFDFDLSIGMFNGAGGSDAYNGTQIVNNLIRVPADLNTTVAPA
                     DANQNIGIHFSFGQNQTIQNNEIQLAGNGVSDSANNRFASSVGMQSNVSGGAVYDGLL
                     IDNNTLNILNAQSADPERILGIWENAHGHSSDITVSNNDFFNDAAGNDPSTNRQSAFR
                     VTSHSSATTVAAYTGNTVDGANIGFEWLTGNNFAGNAAVRLWQNTITNSHTGVLIQSS
                     GMANLFQNSITGSGVAGVDVVSGTLTSSGPVSNAVQENFISGGAGHAIRVGATAGAIG
                     AIFNNDLSGNAGLGVNNLSATLVNASGNWWGTHTPAGVAAEVSANVDYTPWLDVGTDT
                     SATAGFQGSFDAFLVGSDGLGAGNLTLRLNGTDVEFLDDGVLVNSRSLALLTDRLITV
                     NGTNAQTETLLVDYAFGGFFEVDVSFNGGTGAADDDALAVTGGTFTTVTHTFTTTGPE
                     HSGSIVYDASAPAQATISYTGLEPIDMTGSTIANLVFNLPGTNDLAILEDDLIAANGV
                     SQIRSQASTPTFDTTTFSNPTSSLTVNMGGDGGSFTVGSLPDFIAALTINGQAGADTV
                     NFTGTAGFNSLSVTVGGSITDASTASLAVVGNAALSGSSITLGDSAGNVVNFGSLTFT
                     GGAVSITEDSDTVITGINTASSLSLVTASSITDAPGTSLTVSGNARFSGNNITFGDNP
                     ADTTNFGSLTVFAGGNVNIREDSSLEISGANLAQTLILTAEAGAITDTSDADITATGD
                     AILTANGAGGAITLGDQATNDDNFNRLTFNSVGVVSISEDSSTILAGSSTASALSLVT
                     NGSISDSAATSLTVAGNARFSASSTIILGNDDTLNFGTLGFNSTTTVSVREDSAMEIA
                     GVNTAGTLLLTTEAGAITDANDADIAVTGAAILRADGTGGAITLGDEANNLDNFGTVT
                     FNSIGHVSISEDSASALTGIITASSLDFFSANQLTDSGTTTMTVTGNARFATGSLSPI
                     TLGDLTEVLNFGSLTFVSTGAVAITEDSNTVLEGSSTAAVLVLRSAGSITDDADADLG
                     VTGSASFTAGPITLGDQAGNVTNFGSLTFVGGDVSISEDSDAVLTGTGNTANFLNLSS
                     AGSITDAAGTSLTVTSNATFSSTASLTLA"
BASE COUNT         4224 a         7243 c         7075 g         3928 t
ORIGIN      
        1 agcccgtacg cggcgaagcg cctgacgatg ttgctcgatg gcgtagtgaa ggagtacgag
       61 aaacgcttcg gcacgctcag tgtcgagggg cccacggcgc aacagcagaa atgagcggcc
      121 gcgaccatcg tgggtcgctg ccagtgtaga gaaggtagcg ggccgcaaga tcacggcctc
      181 gctgcgcacg tctgtgactc gcttactgtg aagaaatgca cagtttagcg cttggcggaa
      241 gtccttcagg gttttcagca ggttgatgac cgcccctaag aaaatgcttt aggtcggcct
      301 gaccgtgagc cagttcgggc cttccgggaa ctcgactctt gcggggagcc agtaacaaat
      361 acttcaattg tccttccggt ttcacacgag taatatttgc aagtccgctg atgcttgcct
      421 gtgcgttaac ggaactgcag ttcaggggct gcttgtagcg ccagacaggc caacgtttag
      481 gcttggatag caacccgggg gggttccttg caagcgtggc gcggtaattt ctgccttgat
      541 ggccacgcgg cccgccgtgt tctgctagcg gcagcattcc caaacgcccc ctcgcttcac
      601 tgccgccaag cactcgatcc cgctcagcgc cgtggtcctc gcgccagctg gatgaccccg
      661 tttcctaacg agcaatccca cggcgacacg tcgagcagcc ggcgtttcct gtcgccaccg
      721 ttttccggat ggagcatgcc atgagctcaa tatccccgaa attcattccc cattcagaga
      781 aagcggccag cgggctgctg acccggttgc tctcccggct ctggacatct gcgccagaac
      841 gcggacctac gtcccggcgt gcacgcttcg aggcgctgga gccgcgcata ttgttgagcg
      901 cggatctgac gccggagacg gcggacgcgc tggccgacgg cctcaatcgc ctcggcaacc
      961 gcgtcgagga ttttctcgtc agcgagaccc tgctcgacca gcgcatcccg ctcctgctga
     1021 gcgtcaagga gaatgatgat ggcatcgtgg tcgatgaggc gccgaccatc agcgagctct
     1081 acgttgtgcc ggtcgacgcg aacggcgatg gggacataga tgatttcatc gatccgttcg
     1141 acgacgatga atcgatcctg caggacctcg atactgccgg caacgccgac ggcagcgtcg
     1201 acgccggcga attcctgcaa ggctggttct tcgatccact ggaggccttc ctcaacggtc
     1261 atcccgcgac caccaccgac gtcgtcgatt tcctcgaagg cggattcctc ttcagcctgg
     1321 accagtcgct cagcatcggg ccctacacca tcgagtttga aatcgtcgac gcgaaggtcg
     1381 ccgatctcac cgaggatccc gatgcggagg cggcgttctc ggtcggcttt gtgctcagca
     1441 tcacgcagga tctgccgatc gacctcgggc tcgaggcgga cgccctcaag ttgctgccct
     1501 ttaccggacc ggacgcttcg ccggaaccgc cggtcgtcga gacgacctcg tcactttcct
     1561 ttggctttac gttcggtgtg tttaccggcg gccagacgcc ggcacagatc acgggtagcg
     1621 atttcttcat ccgcaaggcc gaccccctgc tggtcagcgt taccgctgag gatacggatc
     1681 tcgacttcaa gctgaacgtc ggtttcctgg gtgccgaagt ggtggatggc agcctcgacc
     1741 tgcaggtgga cgtcgagacc acgctgctcg acccgaacga cccggacgtg ctcggattca
     1801 tcgacacgca gcatggtgtg gaatcgacca ccggcgtcgt caccgcgacg aatgcagtgc
     1861 caagcgcgaa tctcgatcat gcggcgggat tcttcctccg cgtcggcaac gttggcatca
     1921 ccacgccggt gctggtgctc gataatgccg cggccgacct taacgcgctc aagacccatg
     1981 ttgagtctgc gcttgccgcc gccaacctgg acgacctgat tgacgtcagc ctggtcggcg
     2041 ccggcaacga cactttccaa ttctcgctca tcccgacctc cgatacgccg ctcggcttcg
     2101 ccaacgaagc gcttggtctt cttggttccc tgtccggcac accggatgga gatggaccga
     2161 atgcattcga gtatttgtct gaccaggtgt tcctgttgtc ggtcggcggg gggctggcga
     2221 ggatcataac ggtgcgcttc cccgatcctg caaaggaaga gatcgggttc ggtcacgagc
     2281 agacggccga tttgaccgcg gccgcggacc aggtcgttgc cgcaaatacg ccgacggtgt
     2341 tcgacattac cggtaacgcc aacttcacgg tcaccatcac gttcgacaac ggcacgcaac
     2401 tcagccaaag cttcacggta acggcgaccg acaccgacgg cagcggcaat ctcgcacaac
     2461 ttgcgggggc cattgacgcg caacttgtcg cggacaccac gatctttccg ttcgtccagg
     2521 tattggacga gggcggcaag atcaagttcg agggcatcaa ctcgagcgtg agcgggatcc
     2581 gggtgcaggc ttcgggaacc gctaccagtg agatcggctt tgcgtcggac gccgacgcga
     2641 cgctttctct gactgccgcg aacaacgcaa tcaacaccgg cgatctcagc ggaaccgcaa
     2701 ccttcgacgt caccgtcacg acggtcggcg tgggaacgag cacgacgcgt gtgacggttg
     2761 cgccgagcga caacggcggc gtcgcggacc tggctgccga catcgacgcg gcgctcgcgt
     2821 cccagggcct cgcgatcgat gcctccgtgg tcagcggcaa gatcgtgctg acggcgcaga
     2881 acgtcaacgt cgccgcgttc gacgtcaagg ttggcaatca ggacctcgat gacttggtgg
     2941 ccgacgtcaa tgcagcactt gacgacgccg ggctcggaag cgctgtggac gcgtcgaatg
     3001 acggaggcgg tcatctcgtg ctcacctcga ccgcgggcaa gtcgctcgag atcagccgga
     3061 cgctgacctt cgacgccggc gtaaccttta cggagctggg gctaggggcc aatcagctct
     3121 ttagtaccac caccggtgcc gattcccacg tcaccttcga ttttccggtg caggtcctgg
     3181 cgggcctcga ggatgcgacc acggaggcgg acgacgactg ggatcccgcc gacgtcgcca
     3241 tcgtcggcaa cttcgaccca ttcggtccgc tgtccgtgtt cgacgcgggc ggagaaaatc
     3301 gcttcaacct gaacctgacc gtcgacccgc cctcggcggc ggatgcgccg atcgcggtgc
     3361 cgacgccggg ctcgctgacc gatgagatca agctcgtcaa tttcgcagaa ttgctgcaat
     3421 tcaatgtggt cgccgccgag agcatgatcg gcctgatcac ccagctcggc cagtcgctcg
     3481 agagcatagc gagctccggc cagttttccc tgtatggcat tcccttgagc ggctccgcgc
     3541 tcgccgacat gctggtgttc ggtgatctcg tgcaaaccgg gttggtctac gatgtcggcg
     3601 aagacggcca gcttgacgcc acggcgagca agccgaacaa ccacgccgac ctcgtcgccg
     3661 acggcgaagt ctcgcggctc caggtacgca cgatcgtcca cgccgacggc caggatacgg
     3721 tcaagctgct gccgtcgttc gtcaccgcgc agcagctggc atcgcagctc agcacactgc
     3781 tgggaatccc ccttggcccg aacggcatca acgcccgcta cgaccccgcc gacggctcga
     3841 ccaccggcaa tgagctgagc tacacgatca cgctggtctc caaggatcgc atctcggtcg
     3901 gcgtggaaga ctcggtcggc gaggcggatg ccttccatgc gccctttgag ttcgacgtca
     3961 acctgtcgcc gttcggcgag ctcaccgtgg ataccgtgga cgacatgggc aacccgctcg
     4021 actccagggt gacgctcacc agccgcaaca gcctgcagat gacgttcggt gtcgacctcg
     4081 acccggcggc tccgatcgat ttcgcggacg gcggcaccga cgtgcaggac ctgaacaacg
     4141 agaccggtgt cgatatcaag ctcgagcggg cggtcaccgg gacggatccg gtgcgccagg
     4201 tattcggtat cggcagcgat gccacgttcc tcgtgtcgat cgacgacggt gccccggtcc
     4261 aggtcaccct ttcgaaagct gtcgttgacg gtaccgacgg cagcctcaat gcgtttgcgg
     4321 acgagctcga agaggtgatc aacgacgctc tcgccggaga tgccaccgtc tccgtaacgg
     4381 tagtgcccaa tgaagatggt aaggcggagg accggctgag gatcactctc acggccggcg
     4441 gcacgacgat cgatctatct cccgtttcga gtgatccgtc ggctctggtc gagcttggct
     4501 tcgattccca ggctcctgcc gatactgatg gaatctttac tgcgatcaac agcgcaccga
     4561 gcctttatgc cttgacggga aacgcgacgt tttccgtcca ggtcggggcc aatccgacag
     4621 tttcagtgac catcctggcg tccgcgacgg cgggcaacca gtcgatcagc gagctggtcg
     4681 ccgacgtaaa caatgcattg cgtgctgccg gactgctggt tctcgtcggg gacgaagagc
     4741 agggcattcg cgcggcattc gacgccggtc gattggtgct gacggcaacg gcagcggcta
     4801 cccagttctc ggtcacggtg aatggcgcca ccgcagcggg taccgttgcg cacgaggagc
     4861 tggggcttgc gctcggcacc aaggtcgctg acaaggacga tttcatcgtc atcgaccgca
     4921 acggcatcga gcaccgggtc agtctggaca cagcggccga cggcgatgtt caggatctca
     4981 tcgctgtgat caatacggcc ggcacgggct tcttcgaggc cggagcgaat gcctcgggca
     5041 ctggtctgcg tctcacggac atcacgacac cgccgatggg cgtggacaac cggccggcga
     5101 tccgggtggc gacggtcaac ggttcgctgg cggcgctcaa gctcggctgg ctcaccaacc
     5161 cgaccaaccc agacgacatc gacaacgaga acgcgggaga tccgatcaac ccggacttca
     5221 tcgagggtgg gccgatcggc acgcgcgccc tgccggagcg ccttttcgcg cgcgacggcg
     5281 agctgagcgg tgcgttcagc atggaccttc cgggaggcgg cattgccggt gatgcactcg
     5341 tcggcatcgt tggcgtcgac gtcacgatga gcggcgggct cacggccgaa ctcagtgcca
     5401 acctgcaggc gcccggcggc acgccgggca gcaaggtcac gctcgcccag ttgcaggcga
     5461 acgcgggagc ggttccttcc aaccagcaga tcgtcaccga cccgctggtc agcaagatca
     5521 gcacattgaa ctatgacacg gaagtcagct cgtttagcgc cggccggatc gtgcgcggcg
     5581 cgaccagcgg cgccacagcg gtcgttctcg aggtgaccga taacggtagc accggcacgc
     5641 tcacgctgtt cgatatcgac ggcacgttcc aggacaatga ggcgattgtc gacaccgtca
     5701 atgccttaaa cggtgcggcg ctcgccaacg gtggcgttac acagcaggcc tacttcgggg
     5761 agttcgaact cgacttcgca gtgaaggacg gcttcgctga cacaggcttc ggcgacgacc
     5821 tcgaggccgc tctcgacggg ctcacgggca cggtcccggt gcggctcacc ggcttcggca
     5881 acgtcaacac ccatgttgtg ccggcgactt tcacggcgcc ggtggtcgag ttggtggacg
     5941 ttccgggcaa tctcggcgcc ctctacgact tcgtgaacct cggttacggc gatctcaccg
     6001 cggcgctcga gcagctggaa cctctcgtgc atgcactcga ggacgacttt gctgtattgc
     6061 atcagaagtt gccgatcgtc aaccgcagcg tcaacgatct gatccgcatt gcggacggct
     6121 tctcgctatc ggtcgacaac ttcgccgagg tggcggatga cgcatccgcg ctgctccatc
     6181 cggaccagga agacgtgccg acgctcacct tgcagggtgt tgcccaggcg ctgcgcgggg
     6241 ccttcggcct ggggagcaat agcaccggca tcgtggtgga cgtcacctcc gatgggctgc
     6301 tgacggtcga gttcgacctg ctcgagaccg tcagcacgac actcggcctg gacgtggacc
     6361 tgagctcggg cgggctgccc aatctcacca gcggcaaagt gctgcaagta agcggcgaac
     6421 tcggcttggc ggccacgatt ggcatcgacc ttggcgaccc atcgaatgtc tacctcttcg
     6481 aggaaaacgc agggctgtcc ggcaacctgt ccgtgctggg caacggggcc gaagacctgg
     6541 ggctcgtgtt cctcgccatg gtcggcgacg tgccggtact tgtgcgcgac ggcctggtcg
     6601 acatggatct cgaattcacg ctcggcgacc tggatttcag cggcgagtcg acggccggcc
     6661 gcaagtccct gccagcggtc cagttcggcg acttcacggc tgcagtgccg gtcgagagct
     6721 tcattgcgag cgttccgctg ttcgatcctg atgggggact caccggcttc atcggcgaga
     6781 tcgcagcgga cggcgacctg ggcggcctga gcctgccgga tatcgacagc ctggctgatc
     6841 agctcgacga gatcgccagc tcgctgcccg agttcaaccc gctcgacaac ctgctgctgc
     6901 ttgccgagaa catcgacttc ttcctcgaga acctgcagga cttcttcgac ctcgagctgt
     6961 tcggcatcaa gctgcccatc gtcggcgacg atttcgcgga cggcgcgcgc ttcatcgagg
     7021 agttccgcac cagcttcgtc agcgacctgc gcgccaccat cgagtcggcg accgacatcc
     7081 agctcggtga catcaccgat gtcatcgagg atttcttcgc cggggttggc gaaggcggca
     7141 tcgacgtgtc gccgatttca gtggaatcgg atctcgacaa cgaggacttc ggcgagcgtt
     7201 atgcgcagtg gaacttcgag ctgagcgaca cgctgatagt gccgttcgac ctcgaattcg
     7261 acatcggcgt cccgctgctc gggatggaca ttgatatccc gctcatcatc agcatcggct
     7321 ggagcctcga gtggggcttc ggcgtgagct tcgaggaagg cgcgtttatc tacaccggga
     7381 acgacgagga gctttccgtc acggttgaca tcgacctgcc ggcctccgag agcttcagcg
     7441 gcaacctcgc gttcctagag ctcatcgtcg cgaacgccgg cagtggcgtg catctcgagt
     7501 tcgatgtcga cctggagaac ggcaatggcg gcgaccgcct aggcatctcc gatctcggcg
     7561 cgctcgagct aactcccttc gtggagggtg cggcgatcca cgaccatatc cttgacctgg
     7621 acctgacggt cggcatcgtc ggcgtcgcca ctgcatttcc gaatatcacc gccaatttca
     7681 ttctcgactg ggagttgccg cgtactgcgc tcggcgacct caccggcgac gcgctcgcca
     7741 gtggcctcac gaagatcgcg ctcaacgaca tccggctgga tgcggggtcg ttcctgacgg
     7801 acttcctggg ctccacgctg ggcgagatcc gcaagtttac cgagccgttc gacgagatca
     7861 tagacgtgct caccacgccg atcccggtcc tctccgacct ggcgggcgaa gacgtgactt
     7921 tgctcgatat agcgggcgtg ttcggcgaag tcgacgtggg attcatcgaa tggatcggca
     7981 ccgtcctcga catcatcgac accattgggt cgctggatga tgacacccag ctcggcatcg
     8041 acatcggcag cttgacgatt tacgactccg tgggcggcgg cgcaatcggg agcgacttca
     8101 aaggggatct gttcgatgag gacatgaacc tcgccgatag aatcgacctc ggcactgatg
     8161 ccaacccgat cgccgcgctg accaacttcg atccgaattt caacaaccgg ctcaatgcgg
     8221 ctgatgcggg cgagggttcg ggggacgccg cagacgccaa gagcgcgctg caggatctgc
     8281 gcgagggaaa cctcgcggag atcatcagct tcccgttcct ggataacccc ctgagcattg
     8341 tcggcctgct cctcggcgag gacgccatcc tcatccagat ggacgcgcca ccgctgctgg
     8401 tgtcgttcga ctacacccag gtgttcccga tcttcggtcc gctggcggcg acggtcggcc
     8461 tcggcttcaa ggttggcgtt gatttcagtt tcggcttcga taccttcggt gtgcggcgct
     8521 tcgccgatag cggcttcacc aacccggcgc tgatcttcga cggcttcttc ctcggggacc
     8581 gcgagaacgt caccgaaggg gcggacatca acgagctgtt cttcgagttc agcctcaccg
     8641 tgacggccga gctcaatctc gctattgccc gcgccggggc cggcgtgcaa ggcaccgcct
     8701 ttatcggctt cgacctgttc gatcccgaca atgacggagc cattcacctg agtgagctca
     8761 tcaacacgat cgaaaaccag ttgcgcagcg acgacccggg tctcgctccg ctctcgattt
     8821 tcgacctcac cggcaagctg acgctggagc tattcgcctt cctgatcatc gatttcggca
     8881 tcttcgaaat cgattacgag ttcccgatta cgccagaaat cgaggtgttt accttcgacg
     8941 taagcttccc gcggccgccg atcctggcga ccgatctgga taccggtacg ctgcagctca
     9001 acatggggcc caacgccgtc gaccgcctca acggcgacgt gcgcgatggc agcgaggaat
     9061 ttcatgtccg caagacggcc gacggcatcg atgtgtggtc gcccagcctg ggggtgggcg
     9121 atggcagccc gggcgatggc acgcagcact acaccggtga ctttgaccac attgtcgcct
     9181 tggggggcca ggggaacgac cagattcatt tctacgattt cgacgagtcc gacatcactt
     9241 tcgatctcga tggcggcgtc ggcaacgaca tcatccattt ccacgagagt gcggcagtcg
     9301 ctccggcggc cggcgccggc gcgcgcattc gcggtggcgt gggcgacgat gtactaatcg
     9361 gcagccacct cgacgacgag atccacggcg gcgagggcaa tgaccgcatc gagggcggcc
     9421 gcggctacga catcctgttc ggcgacgacg gcacgttggg ggtactgacc aacggtgacc
     9481 gcttcgtcgg caagcgcatc tccgtcaagg acggggacga cgaggtatac ggcggcagca
     9541 gcaccgacca ggacctggcg gacgcggacg cggacgacat catcgtcggc ggtgggggcg
     9601 aggacgagct ccacggcgac ggcggcagcg acgtcatcct cggagacggc ggacgcattc
     9661 tctacgctct gaccgatgga cacatccacg tggccagcct gcggcccgac ccgtttgtgc
     9721 ccaacgacaa ggctgacccc agccccgcgg tatcgaccag ccagaagatc gagaacatct
     9781 tcctgcagct ctccagccat ttcagcggca cggacctggg agacggcgca gacgacgaga
     9841 ttcacggcgg cgcgggcggc gacatcctgt tcggcggcag cgccgacgac gaaatccatg
     9901 gcgacggcgg tgacgactat gtcgtcggcg ggatggggtt cgacagcatc ttcggtggtg
     9961 acaatgacga cacgctgttt ggcaacgccc agaatgacta tatggagggc aacgacggca
    10021 gcgacgtcat gtccggtgat ttcggcgacg acgtgatgca cggcaatgcc ggcgacgact
    10081 acatgagggg cggccgcggt ctcgacgtta tgtacggcga tgaggatgac gacgaggtgc
    10141 tgggcgaagg cgaacccgac gtcctgttcg gcggaatcgg caacgacttg gtcatcggca
    10201 gcgtaggcgc cgacatcatg ttcggcgacg acggcatcgt tgcgaagctc gacaccgatg
    10261 acgtcgggcc gttcaacgac gtctcggtgg gaatcggcaa tccggcgctg ataaccgcgg
    10321 cgatgcgcgc ggctgcggac aatcttgccg gcagtctcga tctcatcctc actgatgccg
    10381 ttgcctcgga cggcaacgac atcctttcgg gcgacggggg cggcgacatc atcctcggcg
    10441 gtggcggcga cgacctgatc ggcggcgacg ttgatccgcg gcttgcgatc gccaacaccc
    10501 cggagaagac gaccgaagcc ggggaggact tcctgatcgg cgacggcggc atcgtccagt
    10561 tcgaccggcg gcgcctgcag tacgtcgcga gtgtcgtgga caacgacccg accagcttcc
    10621 gcgacacgat ctacggggac aacgcgcccg acgtcatcat cggcggccag ggcggcgaca
    10681 gcggcagctt catgcttggc acgcgcgaga ttacgcgcat gctggcgggc ggccacggtc
    10741 ccggccgcgg cgaaaccgac ccggaggtcg gccagaacga catcatcatc ggcgacaacg
    10801 gcgagctgtt ctacgctaac gatgacattg ctgagaactt cggtcgcctt gagcaggtac
    10861 gtacgaccga tacgtcgaat tcgaccggcg gcgcggacat ggcgcacggc gagcttggcg
    10921 acgacatcat ctttggcggt gtgaacggca gcgtggacgt gctgttcgga aacgtcggca
    10981 acgacgtgat gctgggtgac aacggcgagg tggactttgc gtttggccag gacaccgcat
    11041 cgccgctgga cgacgatacc aatctcgaca cgctcgacct gatccgctcc taccgtgacg
    11101 gcctgggtgg caccgacgag atttccggca atgccggcga tgacgtcctc atgggcggca
    11161 cgggcggcga cctgatgtac ggcgacgacg ccatggcttc ggcgggcacc gacgacggcg
    11221 aagacgtcat gctgggtgat aacgccgaca ttttcctcat cggcactacg ggacgcctga
    11281 aggtgaaggt tgcggcgatg accaccggca cggctgtcga tctcatcacg acttccgacc
    11341 ggatcgatcc gctagacccg aaccacgata cggtggctga ggttgaagac gacggcggcg
    11401 ccgacacgat gtcgggcaat gcgaaagccg acatcatgct gggcggggtc aacaacgaca
    11461 acggccttgg ggatccggag gtcgacacga tgtacggcga tcgcgccgcg ccgaccgcgg
    11521 cgtcaatcgt gcttgatagt gacgacatcc ttctgggcga caacggcctg ctcgacttta
    11581 cgttcggcga cagcgaccag aacatcctcg acctgatccg ttccttcgag gacgggctgg
    11641 gtagaacgga tgtcatttcc ggcaacaagg gcttggatgt cgccatcggc ggcagctttg
    11701 acgatcacat ctatggtgac gacactgcgg cgagcgccgc ggcggccgat ctcggcgacc
    11761 tgctgctcgg cgacaacgcc gatgttttcc tcgtcaaccc gaacggagcc agcggcggag
    11821 acaccaagct ggtgctggac gcggcggtga agaccatccg caccacggat gaagagcatc
    11881 ccgagtacgg cggctctgac acgatctcgg gtaacgccaa gggcgacatc attgctggcg
    11941 gcgtgcaggg cgactggctc tatggggatg cggcgagcat ccagcctgcc ttggatggcg
    12001 atgacgtcat cctgggcgac aacggggcct ttgagtggct gtccaccgga cggctcggtg
    12061 aaatcgaggg aatcagcatc gcgctgcata acccgctgct gttcgccaag ttcaacggcg
    12121 atgtggcgga taccgacctc accacgctcg atctgattac caccgaacag cccaccagcg
    12181 gcgggcgcga cacgatccac ggcgacgaag gcgacgacct gatcttcggc ggcaccgacc
    12241 tcgaccggat ccgcggcgac gacggcgacg aggcggcgga gacactttct gcgaatcgcg
    12301 acgtgctgtt cggagaccac gggcgcctct atccgcactt cagtgccctg caggacttca
    12361 actcgcgcaa cttcttcgcc atcgacaccg gcgactcgga cggcggcgag ggtgacgtga
    12421 tgtacggcga ggagggcgac gacgtcatgc tcggtcagca gggcgacgac cgcatgtggg
    12481 gcggcaccca tgccgacgac atgatcggcg gccacaatgt acaaggcggc atcgacgagc
    12541 tctcggttcc cgcggtgcaa gcggtctcga accccggacc ggccaacagt aacgatctga
    12601 tggacggcgg cagtggcaac gacgcgatga agggcgacaa cgtcatcgtc tggcgccgtg
    12661 gcgacgatct atcaccgcgc ttccaggcgc ttgtcggcac gcgcctctac accaccgggc
    12721 ccgacggcgt cgacactatc gtcaccaacg tcagcggcgt ggcacagagc gacccgagcg
    12781 acactgtggg acgtgacgtc acggtggtcg accacgccga cgtggtgccc gccggtgtct
    12841 tcggtgatga cctgatggcc ggcggcgcgg acgatgactt catgctcggc gacctcggta
    12901 acgacgtgat gcagggcgac ggctcgatca gcactgttgc cgatccaggg ccgaacacga
    12961 ttaccactac acacttcgat ggcggcctgc cggactccag cggcacgctg tacttcaacg
    13021 tgcccgaggc ggcgagcgac ggggacgact acatcgaggg cagcggcggc gacgacctgc
    13081 tctacggcgg cctcggccag gacgacatca tcggcggcag ttcctcgctc tttggtctcg
    13141 acaccgagga ggagcgaccg gacggctcgg acatcatctt cggcggcgct ggtacgccgg
    13201 cgcggctcgc gcgcaacgac ttcgtgggcg cgaccgacac cgacaccggc acgggcgagg
    13261 gcgtgggtgc cgtgcccacg ggtgatgacc cgcagatcgc gctcgccgac cgccattcgc
    13321 gcgacgccga cttcatcatg ggcgacaacg cgaatatcta ccgtattatc ggcccgggcg
    13381 acgcgtatcg gcagtttaac tacgaccaaa gctcagtata cgagaaccgc ggcaacgagc
    13441 gcattgtggt gcgcgccatg cagcagctcg actacacgct gggtggtgcc gactatgccg
    13501 gcggctccta tgccggtggt gtggctaatg tcgacaacgg cctcgccgac ctgatccacg
    13561 gcgagtccgg cgacgactat atattcggca tgacgggcag tgatgtgatc ttcggcgaga
    13621 gcgacgacga cgacatcgtc ggcggctacg gcaacgattg gatctcgggc ggcaccggcc
    13681 aagacggcgt gctgggtgac gacggcctgc tctacacgag ccgcaacagc acactgggcg
    13741 agctcctcta cggcgtcgcg ggcctgctgt ctagcgatgc gatgaccaag tacaacaacg
    13801 gcaacgcgct caacgagatc atctccacgc caggcgacgt gcagtacgcc gtgatcaacg
    13861 tcacaggcga gctgaagaag acagcggatc tcgtgccgtt cagcttcgac gcggaatggc
    13921 tcgggctcga cgacgagttt ccggacgacg cgagcaacac gccgttcgcc gacgacatca
    13981 tcttcggcgg cctgggcggg gacttcctgc acggcggttc gggtgacgat gcgatctccg
    14041 gtgccgaggc gctcgagcat gcgtacgtac ccaccttcga cgtcaacggg ctgccgaatg
    14101 gggtcctgga tctcggctac gatgcgttcg acctgccggc cccggtccag ccgggcgatt
    14161 tcgtaccgaa cgtcaatccg ggcaacgtgt tgcacttcaa cccggaggat cttgacgggc
    14221 agcacctcaa caaccgcttc cgcgccgggg agttttacct ctacgacgaa tacgacccgc
    14281 tgcggcgcat cgagttgacg agcagcggcg aactgtggaa gcccggcgac ggcgcgtcgt
    14341 ttgcgttcct gctcaacttc gatcagaccg aaggcatttt tcgcgccagt ggcacggtgc
    14401 ccaaggcgac cggccagcag accgagtcgt acccggcggt cagcgacgat ggcaaggacg
    14461 cgattttcgg cgacctcggc aacgactggc tggtcggcgg tacgggccgc gacaacgtct
    14521 acggcggctg gggcaacgac ctgcttaacg ctgacgacga cctgtcgacc ctcggcgatg
    14581 cgcccaagca gggcgacccg gcggtcgagg gggcgaacga cgttcccgac acgcacccgt
    14641 tctacgagga ccgcgcctac ggcggcgccg ggcgcgacgt cctcatcgcc aacacgggcg
    14701 gcgaccggct gatcgactgg gtcggcgagt acaacagcta cctggtgccg ttcgcgccct
    14761 ttgggcaggc gacagtcagc cgaacgatga tgccgcacct gcacgagttc ctgtacgccc
    14821 tttccgcggg tgatggagcg gatgccacgc gctatgccga tgctatcggc ggcaacgcgc
    14881 cggcaccgac caacaacaac ccgatccctt cccggaacgg cgagccacac ggcgagctgg
    14941 gactggtgct gcagcaggat tttgcctggg gcgaccagac tggagcgccg tctgatcctc
    15001 aggcgggcaa catcccgggc gggccacgcg acgtattgcg ctcggcgggc ttccaggacg
    15061 gctcgatgga gggattcttc gtcgacagcg gcgtattcac ggtgcagagc ggcgtgctga
    15121 aggtggctgc cgcatcgctt ggcggtgacg cggtgagcgt gttccacgtc ggcgacgcgc
    15181 tgcccggtta tttcgaggtg caggcgtcca tcagcgccgt caagcctacc gcgggctgga
    15241 aggcgaacag ctacgtcatt ttcgactacc agggtgaaga ttatttcaag tttgccggta
    15301 ttgacgtgtc caacagcaag ctggtcatgg ggcaccgcga tgccagcggc tggcacatgg
    15361 acgagcaggc gtcggtccag ggcggcttga aggcggacaa ggtctacaac gtattgctcg
    15421 ccattaacgg ggtcaacgcg accttggtag tggacaacaa gaacatcttc tcgcacacct
    15481 accagccacg cgttgtcgac ggctactcgt atggcctgaa taacggcatg atcggggtcg
    15541 gctccgacaa ctcgcgcggc acctttgaca acgtccgcgt gctggtcctg ccgccggcga
    15601 tcaccttcca gtcgacggat gacttcgaga acggggttgc ggacctgttg ccggcatcgg
    15661 gccaggcggg ggcctgggca gtgcaggatg gccgcttgac tggcagtccg gtcgcaggcg
    15721 ggatcgcgac cagccttgtc gacctcggta tcggcggtct cgacgtctcc tcgtacttcg
    15781 agctggcggc gacggtcaac acgcaggccc gcgccgggtt cgtcttcgat cgctacagcg
    15841 cgacggactt caagtttgcc gctatcgacg cggtgaccga ccaggtcatg atcggccact
    15901 acaccgagcg cggcggctgg acggtcgatg cgttcgtatc caagccgatc aatggggggg
    15961 cgagctacca gctcggcgtc gcgatcaagg gctcgacggt cagcgtcacg ctgaacgggc
    16021 aggcggtcct gggcacggtc ttcaacgcgg tcgccgtcga cggcgatttc ggcctgctcg
    16081 cgtcgggcac cccggcgagc ttcgacggct tcatagtgaa gacggacgat gccgcgttcg
    16141 cgcagacgag cggcagcaac atgctggcgg ctacggcggc gccgggcgaa atcgaggcgg
    16201 gcgccacgct cacgcagtcg gtgctggact cgatcgcgac ggtggcgatt acgcagtgga
    16261 ccgatgccct gggcaacggc gacccgagac tggcatctct gggcgacgtg cgcatcacgg
    16321 tggcggacct ggcgggcgat cagctgggct atttcgatgg cgccaacatc ctggtcgacg
    16381 ccgatgcggc cgggcacggc tggttcgtgg acgtgtcgcc ggccgacagc gccgagttca
    16441 gcatccggct cgaccgcaac gtggtggcgg cgaccggctc gagcgacgcg tttgcccgca
    16501 tggacctgct cacggtcatg ctacacgagc tgggccacgc tatcagtttc acggataacc
    16561 agccgggcta cgcggtgatg gacgaggacc tccagtcggg cgtgcgctat ctgctcaacg
    16621 cggataccga cagcccgatg aacgaccggg cgctgctgca gtcggcgtcg aatggggcgc
    16681 gaccggaggc gcggggcggc atgccgggct tcgaccttga cggcggcccg gcggtggcag
    16741 gcggcgccag catcgactgg caggcgccct ccggcgacag ctggggcgtg cgctactcgc
    16801 cctatgcctc cggcaagacg agctccaatt cgtcgggcaa cttctccgac ttcctgttca
    16861 agattttcaa gggcagtgaa acgagcgggt cggcggggta cgacagccta ggcaatgacc
    16921 tgctgggcgc caaggaaggc aaaggcaagc caggggccgg gaacgctcgc tgagcgtgcc
    16981 gggtagttac acaagaggtg acgatccagc tgaccgatcg catcaccatc agcccgtacg
    17041 cctccaagcg gcttacaatg cttctcgacg gggtggtcaa ggaatacgaa aagcgcttcg
    17101 gcgcgctcaa cgtcgaggaa ccaacgccgc agcgatagta ttgttgtaac tgcgtggaac
    17161 aggggtcgcg cagaaggtgt ttggtacgga gggggcagct aagagcctct ccggtcgttc
    17221 gtacagtaga ggaggtgcgt tgatcctgca cgcgccgaca ggccctgcct catggcaggc
    17281 tccgtcgtac tccgggccta tcgcgcccgg acgggtagtg gtgcccacta cctgtgtctg
    17341 ctcgtgcccg gcgctctcgc acccctggga ggcccatcat gagtaacccc aagacatcca
    17401 gctccaaccc gtccacgcgt tggccgagcc tcgccgagat cttcttcggc cgcctcgcca
    17461 atctctggcg caagcgccgc atgcagcgga cctttgccgg ccgccggctc cagtttcagg
    17521 cgatcgagcc gcggatactg ctttctgcgg acttctttgt cgataacccc ggcgactacc
    17581 tagtaactaa tgaccaggga atcatcggcc tgagtaacgg cgacaccgtg acctggaacc
    17641 cgggggcggg cagcgcgcac ggcggccccg tcagcgcttt gacttttggc gtcaatgcgt
    17701 tcggtaggat ccaggacgcc atcaatgccg ccgccccggg cgatacgctt cgcgtcgccg
    17761 gcggcacatt cagcgagctc gttacagtca acaagtccgt ggaagtgctc gggaatcagt
    17821 tcggcgtgga cgctcgcacg cgcgccgtcg tccccgagtc gatcgtcaac ggcgccgact
    17881 tcgggggcgg gcactcgacg tccttcaacg tgaccgccag caacgtggag atcgacggct
    17941 tcaccgtgca gcaggcaacc agcgccaccc agttcggcac cggcatcctc ctgggcgctg
    18001 gaatcagcgg ttcccagata cacaacaaca tcatccagaa caacatcacc ggtctttacc
    18061 tcgccaacgg agcgggcaac caggcggtga tcgagcaaaa cctgttcctc accaacaacg
    18121 tcgccggtcc agtcagcggc accggcatct acacggatca gttcgtcgcc ggcggcaccg
    18181 tctccaacgt gctcatcgac aacaacacgt tcgtcggcaa caccggccag gccatcttct
    18241 tctcctcaac aaacgccgcc gcgcccgcga ccaacatcgt ggtctcgaac aacctgttcg
    18301 acgtcaacgg tcgggcattg gtcgcattca atctggcgac ttcgcagatc gtcggcaaca
    18361 caatgcggaa ctcgacggac tcagccacgg ctgatattcg actatttgaa ggggtcaacg
    18421 acctttcgat cacgggcaac gtgctgcaga acggcgccgg ccaggcggtg cgcatcagca
    18481 acatcggcac cgggtccccc agcgctacgg acgtctcgtt cacacttaac tcgctctccg
    18541 gctacaccgg accgtcgggc gccttcgggg tggacgccgg cgggtacacc ggcatcctga
    18601 atgccactgg caactggtgg ggctccagta ccggcccgac caccgcggcc aatccgggag
    18661 gtaccggcct gaccctcagt gacccgttcg ccgtggtcga cttccagccg tggctgctct
    18721 acggcgattt caacgactcc gcggtcggct tccagattcc cgcaacgttt actgtcgcgg
    18781 cccaaaccga cagtttcacc gccaccaaca acaactaccg ccggatggtc aacgcgatcg
    18841 agtcgctgca acccgggcag acgcttctgc tcagcggcgt cttcgactgg acggaagcga
    18901 acgcctccgc ggcgtgggcg ctgggcaatg acggcacgag tgccaccgtg gacgatttca
    18961 ccctcacggt ccgacctaac ctgaacaacg cagccttgac ggccgccagc ctgggctcgg
    19021 cgcgcatcca gggccccggc gacctggcgg gcgagaacct cgaagggttc ctcgtcttca
    19081 acggcggcga caatcagaac tggacgatct ccaacctgga gatcttcgac ttcgatctga
    19141 gcatcgggat gttcaacggc gccggcggct ccgacgcgta caacggcact cagatcgtca
    19201 acaacctgat ccgcgtcccc gccgacctca acaccaccgt cgccccggcg gacgccaacc
    19261 agaacatcgg catccacttc tcgttcgggc agaaccagac gatccaaaac aacgagatcc
    19321 agctcgccgg caacggcgtc agcgactcgg cgaacaaccg gtttgcctcg tcggtcggga
    19381 tgcagtccaa cgtcagcggc ggcgccgtct acgacgggct gctgatcgac aacaacaccc
    19441 tgaacatcct taacgcccag tcggccgacc ccgagcgcat cctaggcatc tgggagaacg
    19501 cccacggcca cagcagcgac atcaccgtca gcaacaacga cttcttcaac gacgcggcag
    19561 ggaacgaccc gtccaccaat cgccagagcg cgtttcgcgt cacgtcccac tcgtcggcga
    19621 cgaccgtcgc agcgtatacc ggcaacaccg tcgacggggc aaacatcggc ttcgaatggc
    19681 tcaccggcaa caacttcgcg ggcaacgcgg ccgtccgctt gtggcagaac acgatcacca
    19741 acagccacac cggcgtgctg attcagtcga gcggcatggc caacctgttc cagaacagta
    19801 tcactggcag cggtgtcgcc ggggtcgatg tcgtcagcgg cacgctgacc agctcggggc
    19861 ccgtctccaa cgccgtccag gagaacttca tcagcggcgg cgcaggccac gccatccggg
    19921 tcggggccac agcgggcgcc atcggcgcca tcttcaacaa cgacctgtcc ggcaacgccg
    19981 ggctgggagt caacaacctt tcggccaccc tggttaacgc ctcgggcaac tggtggggca
    20041 cccacacccc ggccggcgtg gccgccgagg tcagtgcgaa cgtcgattac accccctggc
    20101 tcgacgtggg caccgacacc agcgccacgg ccggcttcca ggggagcttc gatgcgttcc
    20161 tggttggctc ggacgggctg ggcgccggca acctgactct tcgcctgaac gggacagacg
    20221 tcgaattcct ggacgacggg gtcctcgtca attcccgctc cctggcactg cttaccgacc
    20281 ggctgatcac tgtcaacggc acaaacgccc aaaccgagac cctgctcgtc gactacgcat
    20341 tcggcggctt cttcgaggtg gatgtcagct tcaacggtgg gaccggcgcg gccgacgacg
    20401 acgcgctggc cgtgacgggc ggcacgttca ccaccgtcac gcatacattc accacgactg
    20461 ggccagagca ctcgggcagc atcgtctacg acgcgagcgc tccggcgcag gcgaccatta
    20521 gctacaccgg cctggaaccg atcgacatga ctgggagcac gatcgctaac ctcgtcttca
    20581 acctgccagg cacgaatgac ctggcgatcc tcgaagacga tctcattgcg gccaacggcg
    20641 tttctcagat acgcagccag gctagtacgc ctacgttcga cacgacgaca ttctcgaatc
    20701 cgacctcgag cctgaccgtg aacatgggcg gcgacggcgg ctcatttacg gtcggctcgc
    20761 tacctgattt catcgccgcg ctcacgatca acggccaggc gggtgcagac accgttaact
    20821 tcaccggtac ggcgggcttc aactccttgt ctgtaacagt cgggggctcg atcaccgacg
    20881 cctcaacagc gagcctggct gtggtcggca atgcggcgct ctcgggttcg tcaatcacgc
    20941 tcggcgattc ggccgggaac gtagtgaact tcggctcgct caccttcacg ggcggggcgg
    21001 tgagcatcac ggaggactcg gacacggtaa ttaccggcat caacacggcg agtagcttga
    21061 gcctcgttac cgcgagctcg atcaccgatg ctccgggaac gagcctgact gtctcaggca
    21121 atgcccgttt ttcggggaac aacatcacct ttggcgacaa cccggctgac accacaaatt
    21181 tcggctcgct tacggttttc gcgggtggca acgtgaatat ccgtgaagac agctcgcttg
    21241 aaatctcggg cgccaacctt gcccagaccc tgattcttac cgctgaagcc ggcgcgatca
    21301 ccgatacgag cgacgccgac attacggcga cgggggatgc aatccttacg gcaaacggag
    21361 cgggtggtgc catcaccctg ggtgaccaag ctaccaacga cgacaatttc aacaggttga
    21421 ccttcaactc ggtgggagtg gtgtctatct cagaggactc gtcgacgatt cttgccggta
    21481 gcagcacggc gagtgctttg agtctcgtta ctaacggatc gatcagcgac agtgcggcaa
    21541 caagcctgac cgtggcaggt aatgcgcgat tctcagcaag cagcaccatc attctcggca
    21601 atgacgatac gctcaacttc ggcacgctcg gcttcaattc gaccaccacc gtgagcgtgc
    21661 gcgaggacag cgcgatggag atcgcgggcg tcaacaccgc cgggacgttg ctccttacca
    21721 cggaagctgg cgcgattacc gatgcgaacg acgctgacat tgccgtgacg ggcgcggcga
    21781 ttcttcgggc cgacggaacg ggcggcgcga ttacgctggg tgacgaggcg aataacctcg
    21841 acaacttcgg caccgtaacc ttcaactcga tcggtcacgt cagcatttcc gaggactcgg
    21901 cctcggccct taccgggatt atcacggcaa gcagtctgga tttcttctcg gcgaatcagc
    21961 tcacggactc gggcacgacg acgatgaccg tgaccggcaa tgcgaggttc gcaaccggct
    22021 ccctctcgcc catcacgctt ggcgatttga ccgaggtgct caacttcggt tcgctgacgt
    22081 tcgtttcgac aggcgcagtg gcgatcacgg aggactcgaa caccgtgctc gaaggaagca
    22141 gcactgctgc cgtcctcgtc ctgagatcgg ccggctccat caccgacgat gccgacgccg
    22201 atcttggcgt cacgggaagt gccagcttca cggcaggtcc gatcacgtta ggtgaccagg
    22261 ccggcaacgt gaccaacttc ggctcgctca cctttgtggg cggggacgta agcatctccg
    22321 aggactcgga cgcggtcctt accggcaccg gcaacacggc gaatttcctg aacctatctt
    22381 cggcaggctc aattaccgat gctgcgggga cgagcctgac ggtgaccagc aacgcgactt
    22441 tctcctccac cgcgtcactt acgctcgccg
//
LOCUS       DASUGJ010000617         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2045820, whole genome shotgun sequence.
ACCESSION   DASUGJ010000617 DASUGJ010000000
VERSION     DASUGJ010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2045820"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VFV93_14095"
     CDS             complement(<1..1017)
                     /locus_tag="VFV93_14095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5166530.1"
                     /translation="MQDYRSVSYWLESCGDDLTPRPPLDGSIDVDVAILGAGYTGLWT
                     AYYLLTHDPSLKIALLEKEIAGFGASGRNGAWCTSGFPTGVGTLAERYGLDTALSVQR
                     AMYDAVDEIGRVTESAGIYIDWVKGGSLRIARGRHQLPQIQHSYETYQKLGLGDEYQY
                     LDKQQTDERMSVTDSLGSIYTPHTATIHPGKLVRGLARLVEQMGATIYEQTEVTDFTT
                     GAYPALHTARGHARARNIVLAGESYMSQLPKTRRAVMPAYSLITLTEPLSDADWQQIG
                     WEAHECVSSVAFMVNYLSRTSDGRILFGGRGAPYHFGSSIKDEYDRHPPTHRMLQENV
                     RTWFP"
     gene            1173..2759
                     /locus_tag="VFV93_14100"
     CDS             1173..2759
                     /locus_tag="VFV93_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5166531.1"
                     /translation="MAATYDYIIVGAGSSGATLASRLTEDPDLTVLLLEAGPDYRSAE
                     TPAEMRMPNPSALLRNPEQTCYQWPDLRARHAEGREPQLYWRGRGVGGSSAINGQIAI
                     RPPLEDFDEWADQGCVGWSGEELLPYFNRLEDDLDFGDAPYHGRGGPIPVYRAPLETW
                     GPVDKALREAALALGYGWNPDHNAPFGSGVSPYAINSRDLKRVSTNDAYLEPARERRN
                     LTIIGDALVDRVLIDDSRATGVSVRIGDEWTTFDAGEVILSAGSVYTPPILLRSGVGP
                     ADDLRDLGVEVIADLPVGRDLVDHPIVGLGLHLKPEHRWTSIESRHTNCIVRYASGMA
                     GTGANDMVMLALNLVGYSEEALAMGYIGVSAFQTFSRGWLRITTIDPDAQPDIEIRML
                     SDERDLVRMRDGARRLFEIACQPAVSGICESITAGGVGFAMTSDDSGDALAAMDDDAL
                     DAWMLETVADTQHPVGTCRMGRAGDSNVVVDPACRVLGVDGLRVIDASIMPEVPRSNT
                     HLTCVMIGEAMADRLKRERK"
     gene            2765..3373
                     /locus_tag="VFV93_14105"
     CDS             2765..3373
                     /locus_tag="VFV93_14105"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX5166532.1"
                     /translation="MIDASAEIHRCHWAGSDPLMVAYHDTEWGVPVYDDSALFERLML
                     ECNQAGLSWSTILKKRETFHAAFDGWDAEKIATYGDDDIARLLADPGIIRNRAKVNAA
                     IGNARAYLALQDDGGFSRFVWSFVGGAPLVRATQPAGLADLVATTPESDAMSKALKKR
                     GFTFVGSTICYAFMQSVGMVNDHETGCFRLSEIRSQRQSLSS"
BASE COUNT          574 a         1072 c         1153 g          603 t
ORIGIN      
        1 cgggaaccag gtccggacat tctcctgcaa catccgatgc gtcggcgggt gccggtcgta
       61 ctcgtccttg atgctggacc cgaagtggta cggcgcgccg cgcccgccga acaggatccg
      121 gccatcgctg gtgcgcgaca ggtagttgac catgaacgcg accgacgaga cgcactcatg
      181 tgcctcccag ccaatctgct gccagtcggc atctgagagt ggctcggtca gcgtgatcag
      241 cgagtaggcc ggcataacgg cccggcgtgt cttcggcagt tgcgacatgt acgactcgcc
      301 ggccaggacg atgttcctgg cccgggcgtg gccgcgtgcg gtgtgaaggg cagggtatgc
      361 gccggtcgtg aagtcggtca cctcggtctg ctcgtagatc gttgcgccca tctgctcgac
      421 cagccgggcc agcccgcgca ccagcttgcc ggggtggatc gtcgccgtgt gtggcgtgta
      481 gatcgagccg agtgagtcgg tcacgctcat gcgctcgtca gtctgctgct tgtcgagata
      541 ttggtactcg tcgccaagcc cgagcttctg ataggtctcg taggagtgct ggatctgcgg
      601 gagctggtgc cggccgcggg cgatccgcag tgagccaccc ttgacccagt cgatatagat
      661 ccccgccgac tcggtcacgc gcccaatctc atcgactgca tcgtacatcg cgcgttggac
      721 gctgagtgcg gtgtcgaggc cgtagcgctc cgccagcgtg ccgacaccgg tcgggaagcc
      781 ggatgtacac caggcgccgt tgcggcccga tgcgccgaag ccggcgatct ccttctccag
      841 caacgcgatc ttgagcgatg gatcgtgagt cagcaggtaa taggccgtcc agagaccggt
      901 gtaccccgcg ccgaggatcg cgacatcgac gtcaattgag ccgtcgaggg gcgggcgcgg
      961 tgtcaggtcg tcgccacaac tttcgagcca ataggaaacg gatcggtagt cctgcatcgt
     1021 gccccacccc ttgtcgatcg tgccgccgtc gccgataccg agagccggtt gccgcgtcgg
     1081 agttcgggcg attgtacggc cagaacgtcg gacgggcgac aggaaggtgc tacgatttgc
     1141 cggcgatgcg gcggtgagag cctggagtcg atatggcggc gacgtacgat tacatcatcg
     1201 tgggtgccgg ttcgtcgggc gcgacgctcg cgtcccggct gaccgaggac ccggatctga
     1261 cggttctcct gctggaggcg gggccggact accgctcggc ggagactccg gccgagatgc
     1321 ggatgccgaa cccgtcggcg ttgctacgta accccgaaca gacttgctac cagtggccgg
     1381 acctgcgcgc gcgccacgcc gaaggacgcg agccgcagct gtactggcgc ggtcgtggcg
     1441 tcggcggcag ctcggccatc aacgggcaaa tcgcgatccg gccacccctc gaagatttcg
     1501 acgaatgggc cgaccagggc tgcgtaggct ggtccggcga ggaactgctg ccgtatttca
     1561 acaggctgga ggacgacctg gacttcggcg acgcgcccta tcatggccgt ggcgggccga
     1621 ttcccgtcta ccgggcgccg ctcgaaacgt gggggccggt cgacaaggcg ctgcgcgagg
     1681 cggcgctggc gctcggctat ggctggaatc cggaccacaa tgcgccgttc ggctccggtg
     1741 tctcgcctta cgccatcaac agccgtgacc tcaagcgcgt ctcgaccaac gacgcctacc
     1801 tggaaccagc ccgcgagcgt cgcaatctga cgatcatcgg cgacgcactg gttgaccgcg
     1861 tgctgatcga cgactctcgg gccaccggcg tctctgttcg gatcggtgat gagtggacga
     1921 cgttcgatgc tggcgaagtg atactctcgg ccggctcggt ctacacgccg ccgatcctgc
     1981 tgcggtcggg tgtcgggccg gcggatgact tgcgcgacct cggcgtcgag gtcattgccg
     2041 acctgccggt tggccgtgac ctcgtcgatc atccgatcgt cggcctgggt ttgcacctca
     2101 agccggagca tcgctggacc agtatcgaga gtcgccatac gaactgcatc gtccgctatg
     2161 cctccggaat ggccggcacc ggcgcgaacg atatggtcat gctggcgctg aacctggtcg
     2221 gctatagcga ggaggcactg gcgatgggtt acatcggcgt ctcggccttc cagacgttct
     2281 cacgaggctg gctgcggatc acgacgatcg atcccgacgc ccagccggac atcgagattc
     2341 gaatgctctc cgatgagcgc gacctggtcc gcatgcgcga cggagcgcgg cggctgttcg
     2401 agatcgcatg tcaaccggca gtcagcggga tctgcgagtc gattacggcc ggcggagtcg
     2461 gtttcgcgat gacgagcgac gactcgggcg acgcgctggc ggccatggac gacgacgcac
     2521 tggacgcctg gatgctggag accgtcgctg acacgcagca tccggttggc acctgccgga
     2581 tgggacgcgc cggcgacagc aacgtcgtcg tcgacccagc ctgccgggtg ctcggagtcg
     2641 acggcctgcg cgtcatcgac gcctcgatca tgcccgaagt gccacgttcc aacacacacc
     2701 tgacctgcgt gatgatcggc gaagcaatgg ccgaccggct gaagcgcgag cggaagtgat
     2761 tgcgatgatc gacgcgagtg ctgaaatcca ccgttgccat tgggccggat cggaccccct
     2821 gatggtcgcc tatcacgaca cggagtgggg cgtgccggtc tacgacgact cggcgctgtt
     2881 cgaacgactg atgctggagt gcaaccaggc cgggctgtcg tggtcgacga tcctgaagaa
     2941 gcgcgagacg tttcatgccg cgttcgatgg ctgggatgcg gagaagatcg ccacctacgg
     3001 cgacgacgac atcgcccggt tgctggccga ccccggcatc atccgcaacc gcgccaaggt
     3061 caacgccgca attgggaacg cccgtgccta tctggccttg caggacgacg gcggattctc
     3121 acggttcgtc tggtcgttcg ttggcggcgc gccactcgta cgtgcgactc agccggcggg
     3181 gctggcggat cttgtcgcga cgacgcccga atcggacgcg atgtcgaaag cgctgaagaa
     3241 gcgtggcttc accttcgtcg gctcgacgat ctgctacgcg ttcatgcaaa gtgtcgggat
     3301 ggtgaacgac cacgaaacag gctgctttcg gttgagtgag atccgatcgc agcggcaatc
     3361 cctgtcgtcc tgagccgcag cgaaggacct ctccgtgtca gc
//
LOCUS       DASUGJ010000618         5300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_935025, whole genome shotgun sequence.
ACCESSION   DASUGJ010000618 DASUGJ010000000
VERSION     DASUGJ010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935025"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFV93_14110"
     CDS             <1..735
                     /locus_tag="VFV93_14110"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5166533.1"
                     /translation="ETGGRHGAKYVAENIRTKPDFAIVGEQTFNRVAVGEKGFASTNV
                     VVHGQAAHGALPWEGSNAIEGMAAVILALRDELWPKLEQRTHPYFHHSSASINMIEGG
                     VKQNVVADRCAIYVDRRVIPGEDPAAGVDEIREIAERAVAGLKGIRVEVSAASAGAAS
                     MSDPDSPVVKAMLAANTYLGLNTDLTGFSMGTDGRHFAATGVPTIIYGPGDPALAHVP
                     DEWVGVDEVIGATKAYALVALEMLGD"
     gene            complement(794..1780)
                     /locus_tag="VFV93_14115"
     CDS             complement(794..1780)
                     /locus_tag="VFV93_14115"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX5166534.1"
                     /translation="MVALLIHPGRARRISSILATSIALSLFLTVACGGSGDDDDADNS
                     IANSQPVAAATTAVGSGVMASDPASERTGGAAATSPGETAAVRQQTQGWQQRIIRTAN
                     VTLKVSDDGAGVGSALESVRVLATAKGGYVFSSNSYVEQGRQFAQITIQVPVEQFDAT
                     MNDLRGAAFVEEVVREESSSQDVSEEFVDNESRLNALRETERRFLALLSEAKSVEDIL
                     RLEHELTDIRSQIETIQGRQNYLEQATSFSTITVALQPSGAPTEPQSASGDGFSVSSI
                     AERAWEHSRGAIESILIATITLAIVGAAFLPVAIFCLLAYRVYRNRFRDATL"
     gene            complement(1896..2030)
                     /locus_tag="VFV93_14120"
     CDS             complement(1896..2030)
                     /locus_tag="VFV93_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166535.1"
                     /translation="MDTLAEIGKAILTLGVIGILVILIPILFMFIFTIATSIDERMSR
                     "
     gene            complement(2136..4730)
                     /gene="clpB"
                     /locus_tag="VFV93_14125"
     CDS             complement(2136..4730)
                     /gene="clpB"
                     /locus_tag="VFV93_14125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX5166536.1"
                     /translation="MNPNRLTEKAMEAIATAQQETERRQHATLDVDHLMLALATQQGG
                     VVPRVFERLGVETSAIVSGIEMALANAPKLQFSAQPTLSGNLRNVLQRAEEEARAFGD
                     EYVSTEHLLLAAFQSAPKSESIKLLTSLGITRDTVLEALTEIRGSQRVTDQNPEGRYE
                     ALEKYGRDLTELARLGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIIRGDVPEGLRDKQVVALDFGAMIAGAKFRGEFEERLKAVLKEVTDSDGQIII
                     FIDELHTVVGAGAAEGAMDASNMLKPMLARGELHAIGATTLDEYRKYIEKDAALERRF
                     QPVQVGEPTVEDTISILRGLRERYEVHHNVRIQDSALVAAAVLSNRYITARFLPDKAI
                     DLVDEAASRLRMEITSLPVELDELNRRIMQLEIEREALRKERDEASRKRLDELEKELA
                     NLKESGNALTAQWERERGMLQELSDVKSQIEATRVEIERAQRQADYGRASELQYGQLT
                     QLEQRLAELERQAQTDSGPRLVKEEVDADDIAEVVARWTGIPVSRLVEGEIEKLVQME
                     QRLHARVVGQDEAVVAVANAIRRARAGLSDPNKPLGSFIFLGPTGVGKTELARALAQF
                     LFDDDQAMVRIDMSEYMERHTVSRLIGAPPGYVGYDEGGQLSEAVRRRPYSVVLFDEI
                     EKAHPEVFNVLLQVLDDGRLTDGQGRTIDFRNTVIIMTSNLGSSYLLSNDEEARGRVM
                     EALQANFRPEFLNRIDEVVIFKPLSRDQIGSIVEIQLERVRDRLKERGITLQLTGEAR
                     DWIANRGYDPTFGARPLKRVIQKELLDPLAIKLLAGEIHDGETVTVTAGPDGLVLTGA
                     LSAATLAA"
     gene            complement(4733..5134)
                     /locus_tag="VFV93_14130"
     CDS             complement(4733..5134)
                     /locus_tag="VFV93_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5166537.1"
                     /translation="MRESEPLYVISVASRLLQLHPQTLRKYEREGFVAPSRTTGNLRL
                     YSSQDIDRLRQVKTLVEERGINLAGVQMALQLTGEIRALQAELRNKGRDDYSKIAGAL
                     DELLRMMDATPEVDDQPRGVAIEVQMNRKSR"
BASE COUNT          897 a         1633 c         1731 g         1039 t
ORIGIN      
        1 gagaccggcg ggcggcacgg cgcgaagtat gtcgctgaga acatcaggac gaagccggat
       61 ttcgcgatcg tcggcgagca gacctttaac cgggtcgccg ttggcgagaa ggggttcgcc
      121 tccactaacg tcgtcgttca cggccaggcc gcccacgggg cgctgccgtg ggagggttcc
      181 aacgcgatcg aggggatggc ggctgtcata ctggcgctgc gcgacgagct ctggccgaag
      241 ctggagcagc gcacgcaccc ctatttccac cactcatcgg cgtcgatcaa catgatcgag
      301 ggtggcgtca agcagaatgt cgtcgccgac cgttgcgcga tctacgtcga tcggcgagtc
      361 attcctggcg aggatccggc tgctggtgtc gatgagatcc gtgagatcgc tgaacgggct
      421 gtcgcggggc tgaagggcat ccgcgtcgag gtctcggctg ccagcgccgg cgcggcatcc
      481 atgagtgatc ctgactctcc ggtcgtcaag gcgatgctgg cggcgaacac gtaccttggt
      541 ctgaatacgg atttgactgg cttctcgatg ggcaccgacg gacggcattt tgcggcgacc
      601 ggcgtgccga ccatcatcta cggacccggt gacccggcgc tggcccacgt tcccgacgaa
      661 tgggtcggtg tcgacgaggt cataggagcg acgaaagcct atgcgctggt ggcgctggaa
      721 atgctcggcg actgatgcct ggtagcgtca ggccgtggtg gaggtcacgc cctgacgctc
      781 ctgagttgtc gagttacaac gtagcgtcac ggaagcggtt ccggtatacg cggtaggcca
      841 acaagcagaa gatcgcgacc ggcaggaatg ctgcgccgac gattgcgagg gttatcgttg
      901 ctatcaggat gctctcgatc gcgccgcgtg agtgctccca cgcgcgttca gcgatactgc
      961 tgacactgaa cccatcgcca gaggccgact gaggctcagt cggtgcgccg gacggctgaa
     1021 gggcaaccgt gatcgtcgag aacgacgtcg cctgttcgag gtagttctgg cggccctgaa
     1081 tcgtctcgat ctgcgagcgg atgtcggtca gttcatgttc gagacgcagg atatcctcta
     1141 cggacttcgc ctcgctcaag agtgccagga agcgacgctc cgtctcgcgc aacgcgttca
     1201 accggctctc gttgtcgaca aactcctcgc tgacatcctg acttgaagac tcttcgcgga
     1261 cgacctcctc gacgaacgcg gcgccgcgca gatcgttcat cgtcgcgtcg aactgctcga
     1321 cgggcacctg aatggtgatc tgggcgaact gacgcccctg ttcgacgtaa gaattcgatg
     1381 agaagacgta gccacctttc gccgtcgcca gcacgcgtac ggactcgagt gcgctgccga
     1441 ccccagcgcc gtcatccgat accttcagcg tgacgttggc ggtgcgaatg atgcgctgtt
     1501 gccagccctg cgtctgttgg cgaacggcgg cggtctcgcc gggcgacgtc gcggctgctc
     1561 cgccggtgcg ttcgctcgcc ggatcggatg ccatcacgcc ggaaccgacg gcggtcgtcg
     1621 cagctgcgac cggctggcta ttcgcaattg aattgtcagc gtcgtcgtcg tcgccggaac
     1681 cgccgcaggc gacggtcagg aagaggctga gggcgattga tgtcgcgagg atcgatgaga
     1741 tgcggcgggc gcggcccgga tggatgagta gggcaaccat gtcgtccacc actttcgatc
     1801 gcgagactcg aatgcacagt gagttgacgc gtctgagggg tagacgatgg atgtacgacg
     1861 ttggttcccc gaggatcgaa ccatctggcg cgaggctagc ggctcatccg ctcgtcgatg
     1921 ctggtggcga tcgtgaagat gaacatgaac aggatcggaa tcaggatcac caggatgccg
     1981 atcacgccga gcgtgagaat agccttgccg atctcggcca gcgtgtccat tgtgacgact
     2041 cgccttcact cgtaacggcg cgaggcgccg gaacacgacg aacgggcgat cggttattgc
     2101 cgatcgcccg taagtatacg cggtgtgttt ggggcttacg ccgccagcgt cgcggccgac
     2161 agggcgccgg tcaggacaag accatccggg cctgcggtga cggtgaccgt ctcgccgtca
     2221 tgaatctcac cggccagcag ctttatcgcc agcgggtcga gcaattcctt ctggatgacc
     2281 cgcttcaatg ggcgggcgcc gaaagtcggg tcgtagccac ggttggcgat ccaatcgcgc
     2341 gcttcgccgg tgagctggag cgtgatgccc cgttccttga gccggtcgcg gacccgttcg
     2401 agctgaatct cgacgatcga gccgatctga tcacgcgaga gcggcttgaa gatgacgacc
     2461 tcgtcgatcc ggttgaggaa ctccggccgg aagttggcct gtagcgcctc catcactcga
     2521 ccgcgcgctt cctcgtcgtt gctgagcagg taggacgagc cgaggttgct ggtcatgatg
     2581 atgaccgtgt tgcggaagtc gatcgtccgc ccctgaccat ctgtcagccg gccgtcgtcc
     2641 agcacctgaa gaaggacgtt gaacacttca ggatgcgcct tctcgatctc gtcgaatagg
     2701 acgaccgagt acgggcgacg ccggacggct tccgagagtt ggccgccctc gtcgtagccg
     2761 acgtagcccg gaggcgcgcc gatcaaccgt gagacggtgt ggcgctccat gtactccgac
     2821 atgtcgatcc ggaccatcgc ctggtcatca tcgaacagga actgggccag tgcccgcgcc
     2881 agctcggtct tcccgacacc ggttgggccg aggaagatga acgagccaag cggcttgttc
     2941 gggtcggaca ggccggcccg cgcccgacgg atcgcgttgg caacggccac gacggcttcg
     3001 tcctggccaa cgacgcgggc gtgcagccgc tgctccatct ggaccagctt ctcgatctcg
     3061 ccctcaacca accggctgac cgggataccg gtccagcgcg caacgacctc ggcgatgtcg
     3121 tcggcgtcta cttcttcctt gaccaagcgg gggccgctgt cggtctgcgc ctgacgctcc
     3181 agttcagcga gccgctgctc cagttgcgtg agctgtccgt attgcagctc cgaggcgcgg
     3241 ccgtagtcgg cctggcgctg ggcgcgctcg atctcgacgc gggtggcttc gatctgcgac
     3301 ttcacgtcgg agagctcttg caacatgccc cgctcgcgct cccactgggc tgtcagggcg
     3361 ttgccggact ccttgaggtt ggcgagctcc ttctcaagct cgtcgagccg cttgcgggat
     3421 gcctcgtcgc gctccttacg gagcgcctca cgctcgatct cgagttgcat gatgcggcgg
     3481 ttcagctcgt ccagctcgac aggcaggctg gtgatctcca tccgcagccg cgaggcggcc
     3541 tcgtcgacga gatcgatcgc cttgtcgggc aggaagcggg cagtgatgta gcggtttgag
     3601 aggaccgctg cagcgaccag tgcgctgtcc tggatgcgga cgttgtggtg gacttcgtag
     3661 cgttcacgca ggccacgcag gatcgagatg gtgtcctcga cggttggctc gccaacctgg
     3721 acaggctgga agcgtcgttc gagggcggca tccttctcga tgtacttgcg gtactcgtcg
     3781 agcgttgtcg cgccgatggc gtgcaactca ccgcgcgcca gcatcggctt gagcatgttg
     3841 ctggcgtcca tcgcgccctc ggcagcgccg gcaccgacga cggtatgcaa ctcgtcgatg
     3901 aagatgatga tctgaccgtc cgaatcggtg acttccttga ggaccgcctt cagacgctcc
     3961 tcgaactcac cccggaactt ggcgccggcg atcatcgccc cgaaatcgag cgcgacgacc
     4021 tgcttgtccc gcagcccttc cgggacgtcg ccccggatga tgcgctgggc aagcccttcg
     4081 acgatggccg tcttaccgac gcccggctca ccgatcagga ccgggttgtt cttcgtccgt
     4141 cgcgagagga cctggatcac gcgtcggatt tcctcgtcac ggccgatgac cgggtcgagc
     4201 ttgccgagcc gcgccagctc ggtcaggtcg cggccgtatt tttcgagcgc ttcgtagcgt
     4261 ccttcgggat tctggtcggt gacgcgctgg gacccacgaa tctcggtcag cgcctcgagc
     4321 accgtgtcgc gggtgatgcc gagcgaggtc agcagcttga tcgactcgct cttcggcgcg
     4381 ctctggaacg ctgccagcag caggtgttcg gtcgagacgt actcgtctcc aaacgcgcgg
     4441 gcctcttcct cggcgcgctg gagaacgttg cgcaagtttc cgctcagcgt cggctgggcg
     4501 gagaactgca actttggcgc gttggcgagc gccatctcaa taccgctgac gatcgcggac
     4561 gtctcgacgc caaggcgctc gaagacgcgc ggaacgacgc cgccctgctg ggtggcgagg
     4621 gcgagcatca gatgatcgac atccagcgtc gcatgctggc gtcgttcggt ttcctgttgt
     4681 gcggtggcga tggcttccat cgccttctca gtgagacgat tggggttcat ggttatcgtg
     4741 acttcctgtt catttggacc tcgatagcga cgccgcgcgg ctggtcatcc acctctggtg
     4801 tggcgtccat catccgcagc aattcgtcga gcgcgccggc gatcttgctg taatcgtcgc
     4861 gccccttgtt gcgaagttct gcctgcaacg ctcggatctc gcccgtcagt tgcagcgcca
     4921 tctggacgcc tgccagattg atgccgcgct cctcgaccag ggtcttgacc tgacggagac
     4981 gatcgatgtc ctggctcgaa tacagacgca ggttgccggt cgttcgcgac ggcgcgacaa
     5041 agccttcgcg ctcgtacttg cgcagagtct gcggatgcag ctgcaggagc cgtgacgcga
     5101 cgctgataac gtagagtggc tcgctctctc gcatgcagcc tccaatagtg agcgatgatc
     5161 gcaacttcct tgacatggag tatatcagag tcgcagaatt cgatcaagcg cacacgtctg
     5221 gattctcacg tgcgcatcca gcgagattca tgggtggtta gagggtgtta tggacttccg
     5281 gcgagcagca gtttcatgac
//
LOCUS       DASUGJ010000619         5462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1906492, whole genome shotgun sequence.
ACCESSION   DASUGJ010000619 DASUGJ010000000
VERSION     DASUGJ010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906492"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2444)
                     /locus_tag="VFV93_14135"
     CDS             complement(<1..2444)
                     /locus_tag="VFV93_14135"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5166538.1"
                     /translation="MNARVIRLLLIDDETDPGANVHVLDVAAACGFEASLAAGWHDGL
                     ALLAVGAVDLALVAERIGGRSGLDLIIAATERGYDVPLILLSDDDRAFGHDALEAGAV
                     DYVVRSGLTPALLDRAARYSIGQARAARAQRASEARFRAMYDNSTHIIGVVSPDGRLL
                     QVNRALETIAGIRPDDLIGTNAIDRVHPDDVSVAVAALSSISSQPGGTATVRYRFQHA
                     DTSWRWFEATLTNLLDEPAIAGLFSTARDITEQVEAEERLHKQAEQLQLLTEMSATFS
                     ASALDLDAILESLARRVSEVLDDICILRLVSDDGLLLNPVAVWHPDPKLRALLREVHI
                     RPHGVGEMLPGEVMRSGEPLLLPEFDSFEELRRHPEKWPFFQSHGVASMLIVPLRVRG
                     TIIGTLAVSRITRNRPYSSDDRDFLQDLADRAAQAVDNARLYRHALDAETQHRQLIEQ
                     LPAVIFVEAIDDRQGNGIGTTLYMSPQCADVFGMTADEWMQQRDPWINIVHPDDRDRL
                     LGEIQRTNETGDPFSVQYRAIRPDGRVVNVECEARLVCDSDGIPRVWQGFALDVTQRQ
                     AVEAALHEAEDQFRTLVEYSPAVIFTSAADEESTPLYMSPQIEQMLGYPPEAWTTGDI
                     WDEIIHPDDLEWVTQLDKETAATLAPYDAEFRVFASDGRLVWLRNYSVAVCDEDGNPR
                     YWQGYLIDITAQKVAEEAIRFQAQLLDTAGQSIIAADIDGKITYWNRAAEALYGWRSD
                     EVRGRNVFEITPVDQDYDWTGLMEKLQDGEIWTGEFSARRKDGSIFPAFVTSSPVCDD
                     DGTLIGIIGVSTDISER"
     gene            2654..3430
                     /locus_tag="VFV93_14140"
     CDS             2654..3430
                     /locus_tag="VFV93_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5166539.1"
                     /translation="MGDIQVGDVSIHYALDGAGPWLTLVHGFSQDLRIWQPQVERLAG
                     SFRLLRIDLRGHGGSARPDGGYGPVEYATDILAVLDALEIGATHYWGTHTGAGVGLLL
                     AVQHPERIATLVLDGAVIPGQPEPSVDGWQARAREIARDQGMPAALAVWFDDSPFFAT
                     TQRPAAHRAIISDFSGAPWLANGPALPVPDVAALLPSIRQRTLIVNGASDLPSFLTRA
                     ADLEQTIPNSRRYVIPNAGPFAAWDAPDAVTPLVAQFLDA"
     gene            3533..5338
                     /locus_tag="VFV93_14145"
     CDS             3533..5338
                     /locus_tag="VFV93_14145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX5166540.1"
                     /translation="MPEPTATLPRWDMSVVYPSLDSPEFEAGLASVVAGIDGLAELFD
                     QNGVERRDPSPLDAATVAAVDEVIERMNAVIEAAANLGSYISAFISTDSRNDRAQAIM
                     SGYQQQGARLQKLETRFTAWVGSLDVDGLIERSELAAAHAFALQQAKRRAAHLMSPAE
                     EDLAAELNLSAGSAWAKLYNNLTSQIGVPFEQDGAGVELPMSAIRNLAHERDRETRRR
                     AYEAELAAWQQHALPLASAINGIKGQVNTLATRRGWPSPLDEACFNNHIDRATLDAML
                     TAARESFPDFRRYLRAKARALGLDSLTWYDLFAPLGESPRVWTWDEARAFIVRHFHSY
                     SQRMGEFAERAFNENWIDAEPRPGKRDGAFCMPLRADESRILANFKPSFSGVTTLAHE
                     LGHGYHNINRCKVTILQRTTPMALAETASIFCETIIRDAGLQEADEAEALAILEGSLQ
                     GNCQVVVDITSRFLFEQAVFEQRANRELAIDELNEIMLDAQRQTYGDGLDMDVLHPYM
                     WAVKGHYYSAGRSFYNFPYMFGQLFALGLYAQYQRDPETFRASYDDLLMSTGLDDAAT
                     LANRFGIDIQTPAFWRASLDIIRADINRFEEIVGK"
BASE COUNT          944 a         1879 c         1721 g          918 t
ORIGIN      
        1 cgctccgaga tgtcggtcga gacgccgatg atcccgatca gcgtgccgtc gtcgtcgcag
       61 accggggaac tggtgacgaa ggctgggaag atgctgccgt ccttacgccg cgccgagaac
      121 tcgccggtcc agatttcgcc atcctgcaat ttctccatca gcccggtcca gtcgtagtcc
      181 tggtcaaccg gcgtaatctc gaagacattg cgaccgcgca cctcgtcgga ccgccagcca
      241 tacagcgctt cggcggcgcg attccagtag gtgatcttcc cgtcgatgtc ggccgcgatg
      301 atggactggc cggccgtgtc aagcaattgg gcctggaagc ggatcgcctc ttcggcaacc
      361 ttctgggcgg tgatatcgat caggtagccc tgccagtaac gcgggttgcc gtcctcatcg
      421 cagaccgcga ccgaatagtt gcgcagccag accagccggc catcgctggc gaagacacgg
      481 aactcggcgt catacggcgc cagcgtcgcg gcggtctctt tgtcgagctg ggtcacccac
      541 tccaggtcat cggggtggat gatctcatcc cagatatccc cggtcgtcca tgcctcgggt
      601 gggtagccca gcatctgctc gatctgcggg ctcatgtaga gcggtgtcga ttcctcatcg
      661 gcggctgagg tgaagatgac ggccgggctg tactcgacca gcgtccggaa ctgatcctcg
      721 gcctcgtgga gcgccgcctc aacggcctgg cgctgcgtga cgtcaagcgc gaaaccctgc
      781 cagacgcgcg gaatcccatc gctatcacaa acgagccgtg cctcgcactc gacgttcaca
      841 accctgccgt ccggccgaat cgcccgatac tggacgctga acgggtcgcc ggtctcgttg
      901 gtgcgctgga tctcgcccag cagccggtcg cggtcgtccg ggtgcacgat gttgatccac
      961 gggtcgcgct gctgcatcca ctcgtcagcc gtcatcccga agacatcagc gcattgcggg
     1021 ctcatgtaga gcgtcgtccc gatgccattg ccctgcctat catcgatcgc ctcgacgaag
     1081 atgacggccg gcaattgctc gatcaactgg cgatgctggg tttcagcgtc gagcgcgtgt
     1141 cggtagagcc gggcgttgtc gacggcctgg gcggcgcggt cggccagatc ctggaggaag
     1201 tcgcggtcgt cgctggagta gggccggttc cgcgtgatgc gtgacaccgc caatgtcccg
     1261 atgatcgtgc cacgcacccg gagcgggacg atcagcatgc tggcgacgcc gtgcgactga
     1321 aagaacggcc acttctccgg atggcggcgc agttcctcaa atgagtcgaa ttccggcagc
     1381 agcagcggct caccgctgcg catgacctcg cccggcagca tctcgccgac gccgtgtggc
     1441 cggatatgaa cctcgcgcag tagcgcgcgc aacttgggat cgggatgcca gacggcaacc
     1501 gggttgagca gcaacccatc gtcgctgacg agccgcagga tgcagatgtc gtccagcact
     1561 tcgctgaccc gccgtgcaag cgactccagg atcgcatcca gatcgagcgc cgaggccgag
     1621 aaagtcgccg acatctcggt cagcaactgc aactgctcgg cctgcttgtg cagccgttcc
     1681 tcggcctcga cctgctcggt gatatcgcgc gcggtcgaga acaggcccgc gatggcgggc
     1741 tcgtcgagca ggttggtcaa cgtcgcttcg aaccagcgcc aggacgtatc ggcatgctgg
     1801 aaccggtagc ggacggtcgc tgtcccaccc ggctgcgacg agatcgaact caatgcggcc
     1861 actgcgacgg agacatcgtc gggatgcacc cggtcaatcg cgttcgttcc gatcagatcg
     1921 tccggacgga tgccggcgat cgtctccaac gcacggttga cctgcagcag ccggccatcc
     1981 gggctgacga cgccgatgat gtgggtggag ttgtcgtaca tcgcccgaaa gcgggcctcg
     2041 ctggcccgct gggcacgggc cgctcgcgcc tggccgatgg aatagcgtgc cgcgcgatcc
     2101 agcaacgccg gtgtcaggcc gctccggacc acgtagtcaa cggcgccggc ttcgagggca
     2161 tcatgtccga acgcacgatc gtcgtcggac agcagaatca gcgggacgtc gtacccacgc
     2221 tcagttgcgg cgatgatcag atcgagtccg ctacggccgc cgatccgctc ggcgaccagc
     2281 gccaggtcga cggcaccgac cgcaagcagt gccagcccgt catgccagcc ggcggccagg
     2341 ctggcctcga aaccgcaggc agcggccacg tccagcacgt ggacgttcgc ccccgggtcc
     2401 gtctcatcgt cgatcagcag cagtctgata acccgcgcgt tcatcacgtt ccagatgcct
     2461 gaatgcggaa ccggctgctg tctgccggcc gttgctgtgc tcgcaagtct ggtgtatcac
     2521 gcatcgttca ccttcacgaa cagccatcgg taccggatac tggtagccgt tcgcatctgt
     2581 gacacttctg tgacatgggc gcgatgcgta tccttccggc tgggcattgg ggatgggaac
     2641 agggagcgta tccgtgggcg atatccaggt tggggacgtc tcgattcact acgcgctgga
     2701 cggcgccggc ccttggctga cgctcgtgca cggcttctcg caggacctcc gcatctggca
     2761 gccgcaggtc gagcggctgg ccggctcgtt ccggctgctg cggatcgacc tgcgcgggca
     2821 tggcggctcg gccagacccg acggcggcta cggcccggtc gagtacgcca ccgacatcct
     2881 ggccgttctc gacgcactgg agatcggtgc gacccattac tggggcaccc acaccggcgc
     2941 gggcgtcggg ctgctgctgg cagtccagca ccctgagcgg atcgcgacgc tcgtcctcga
     3001 tggtgccgtc atccccggtc aaccggagcc gtccgtcgac ggctggcagg cacgcgcccg
     3061 cgagatcgcc cgcgaccagg gcatgccggc cgcgctggcc gtctggttcg atgactcacc
     3121 gttcttcgcg accacccagc gccccgccgc ccatcgagcg atcatcagcg acttcagcgg
     3181 cgcgccgtgg ctggcgaacg gcccggcgct gccggttccc gacgtcgcgg cgctgctgcc
     3241 gtcgatccgc cagcgcacgc tgatcgtcaa cggcgccagc gacctgccct cgttcctgac
     3301 gcgcgcggcc gacctggaac agaccatccc caacagccgc cgctacgtca tcccgaacgc
     3361 cggccccttc gccgcctggg acgcccccga cgccgttaca ccgttggtgg cgcagttctt
     3421 ggacgcgtga cgcgggatgc gtgacgcgga ggttgaaacc cgtcatccgc gtcacgcgtc
     3481 acgcatcccg tatcccgcat cacgcgttat gcaataccga aaggacaccc gcatgcctga
     3541 acccaccgcg acgctgcccc gttgggatat gagcgtcgtc tatccgtcgc tcgactcgcc
     3601 ggagtttgag gcgggcctgg cgtccgtcgt cgccgggatt gatgggctgg cggaactgtt
     3661 cgaccagaac ggcgttgagc ggcgcgatcc gtctccgctg gatgcagcga cggtcgcggc
     3721 cgtcgatgag gttatcgagc ggatgaacgc cgtcatcgag gcggcggcca atctgggtag
     3781 ctacatctcc gccttcatct cgaccgactc acgcaatgac cgcgcgcagg cgatcatgag
     3841 cggctaccag cagcagggcg cgcggttgca gaagctggag acgcgcttta cggcctgggt
     3901 cggctcgctg gacgtcgatg gactgatcga gcgatccgag ctggcagccg cccacgcctt
     3961 cgcgctccag caggccaagc ggcgggcagc ccacctgatg tcaccggccg aggaggatct
     4021 ggccgccgaa ctgaacctct cggccggctc ggcctgggcg aagctctaca acaacctcac
     4081 ctcgcaaatc ggcgtcccct tcgaacagga cggtgccggc gtcgagctgc cgatgagcgc
     4141 gatccgcaac ctggcccacg agcgcgaccg cgagactcga cgccgcgcct acgaggccga
     4201 gctggctgcc tggcagcagc acgcgctccc gttggcatcg gcgatcaacg gcatcaaggg
     4261 ccaggtgaat acgctcgcaa cccggcgcgg ctggcccagc ccgctcgacg aagcctgctt
     4321 caacaaccac atcgaccgcg ccacgctcga cgcgatgctg acggccgccc gcgagtcgtt
     4381 ccccgacttc cgccgctacc tgcgcgccaa agcccgcgcg ctcggactgg acagcctgac
     4441 ctggtacgac ctgttcgcac cgctcggcga gtcgccccgc gtctggacct gggacgaggc
     4501 acgcgccttc atcgtccgcc acttccacag ctactcgcag cggatgggcg agttcgccga
     4561 gcgcgctttt aacgagaact ggatcgacgc cgagcctcgc cccggcaagc gcgacggcgc
     4621 cttctgcatg ccgctgcgag ccgacgagtc gcgcattctg gccaacttca agccatcctt
     4681 ctccggcgtt acgacgctcg cccacgaact gggccacggc taccacaaca tcaaccgctg
     4741 caaggtgacg attctccagc ggacgacacc gatggcgctg gcagagacgg ccagtatctt
     4801 ctgcgagacg atcatccgcg acgctggcct ccaggaggca gatgaggccg aggcactggc
     4861 gattctcgaa ggatcgttgc agggcaactg tcaggtggtc gtcgacatca ccagccgctt
     4921 cctcttcgaa caggctgtct tcgagcaacg cgcgaaccgc gaactggcaa tcgacgagtt
     4981 gaacgagatc atgcttgacg cccagcggca gacgtatggc gacggactcg acatggacgt
     5041 cctgcatccg tatatgtggg ccgtcaaggg gcactactac agcgcgggac gctcgttcta
     5101 caacttcccg tacatgttcg gccagctctt cgcgctcggc ctctacgccc agtaccagcg
     5161 cgacccggag accttccgcg cctcctacga cgacctgctg atgtcgaccg gccttgacga
     5221 cgccgccacc cttgccaacc gcttcggcat cgacatccag acccccgcct tctggcgcgc
     5281 cagcctcgac atcatccgcg cggatatcaa ccggtttgag gagattgtgg ggaagtaaaa
     5341 gtgctaagtg agccagccga acggcgtcgc tgaggaacag cactgcatcc cctgtcatcc
     5401 tgagccgcag cgaaggatcg ctgaacgtac gagacacatg cgcgctaatc gctagaacgg
     5461 cg
//
LOCUS       DASUGJ010000620         4462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1350754, whole genome shotgun sequence.
ACCESSION   DASUGJ010000620 DASUGJ010000000
VERSION     DASUGJ010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350754"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..3244
                     /locus_tag="VFV93_14150"
     CDS             332..3244
                     /locus_tag="VFV93_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5166541.1"
                     /translation="MSLTIAEALQQARQAIDSGDYGSAATTCTRLVSQFPGYASAHRL
                     LGEIYREQGQPDDAERSFAAALTRQPRQAETCLGLGLIAEEHGSSEAALAFCQVAWEL
                     APNQTALREPLTRVSLRRYGSDGELQFTHAALAQLHANASRLRRAVAEYRLALAQLPA
                     RVDLQLGLLEALWRLGDDSEAIQRARGIVEQQPECAPALVILADLEQRSGNTRRAEDL
                     LKRLRGVDPDGAIVAGMIALNPRSNQQFLALAPAAMPFVDEQLAAAAQERPKIAPAPD
                     FDYRPTRSEIPTPDIDELAPMSFEEFGAADAESPAASPADVAAFNFDEFSDLPEGLEP
                     ISFEELGALSDEQATGESADFEMAAFDTAPEAEAPAPFTLEEVLQFESPAAETPADDP
                     QQLVADDAAFIAEPAATMQEQPAAGIDDLASLAAALEGDVADALNRAGEPYDLAAGVG
                     ESRPPAATPRGYTTVLRGLEDQGLAPFDPRSREPIEGMPSMLPDEDPIFEEPAPAASD
                     ESPAQTDEFGKITRDWDTIDDEIQAALPVQPGRGYTEELRALDEIGLTPFEVEDDEDA
                     VAGVTPFNPFASEQLAVDPSMPEPVVDERPAAVAEPAPADAATDELLGGLEPFAFEEF
                     DSDQSTAGDTQTRSFFNQGGWDSSSSAVPSDADLDALLAYEDEEPLPVAEPEPEPAPE
                     PVAAAEPEIALDDASFADAMASDQEPAATASEAANLATPPGLSEADLNEFFESSVAVT
                     RQLPGEAEHPMFQADAPLAEAVAAEQAAGSEPAAGEPAEELQPQSLTSALRPNTEIFT
                     RARAVKLGLMSEGRINGTRELGEGITTDELMAMEERLKTRNLPQLDTDDFDDADDEDD
                     LVAATGATRDTRTLRAALEVTPDDDELRWWLAEALRDRGDLDDAYSEYRWLIRHAPHR
                     HDQILDALTESVDRDQMPETAHRLLGDIYRRRGDVTRASSHAALALQMRRRSGRVV"
     gene            3287..3625
                     /locus_tag="VFV93_14155"
     CDS             3287..3625
                     /locus_tag="VFV93_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX5166542.1"
                     /translation="MVDESPAGGLRDQLMQMLEYRVVLGAVVTTMDGLVITYAGLGPD
                     DAEMLAAASSSQPDDNQYSSDKTGGGTLHVLRGNDVRLILLTDSDAPQPAVTDLMSQH
                     LGNLEDSLAV"
     gene            3806..4267
                     /gene="ndk"
                     /locus_tag="VFV93_14160"
     CDS             3806..4267
                     /gene="ndk"
                     /locus_tag="VFV93_14160"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX5166543.1"
                     /translation="MASNDRTLIIVKPDGVQRGLIGEILSRLERRGLKFVGLKQITMS
                     RELAEEHYAGLKDKPFYPKLVDYITSAPVVVGVVEGPNAFQTVRDTIGATDPLKAAPG
                     TVRFEFGISIGRNLSHASDGDESAVREINLFFSPDELSSWARDTDAWILEE"
BASE COUNT          786 a         1493 c         1491 g          692 t
ORIGIN      
        1 acgcacctgc tcatccccta tgccccatcc cccgcccgcg tggtacccag gttgcggcaa
       61 atggccccag acggccgaga ctcgtcgatt tgatggccca ttcgtcctgc cggcgctggt
      121 tttgggagta ttgggctacc gatctcgatt gagcgacaac ggtctccttc tataatgcaa
      181 atgccacgtt ttgttgacga aggatgggga ttgttcgata tccagcatcc atccgtacac
      241 gaggacaatc gcgccgttgg gttagcggcc agttgaagcc agcacgccag ggatcgaatc
      301 acgcaggggg ctggcccagg gatgagcgaa gatgagtctt acaatcgctg aggcattgca
      361 gcaggcacga caggccatcg acagcggcga ctacggttcg gcggcgacga cctgcacgcg
      421 actggtgagc cagtttcccg gttacgcgtc tgcccatcgc ctgcttggcg agatctaccg
      481 tgagcagggg cagccggacg acgccgagcg ctccttcgcg gcggccctga cgcgccagcc
      541 acgccaggct gaaacctgcc tgggccttgg cctgatcgcg gaagagcacg gctcgtccga
      601 ggccgcgctc gccttctgcc aggtcgcctg ggagctggct ccgaaccaga ccgcgttgcg
      661 cgaaccgttg acgcgtgtct cgctgcgccg ctatggctcc gatggggaat tgcagttcac
      721 ccacgccgcg ctggcccagc tacacgccaa cgcctcgcgc ctgcgccggg ctgtcgccga
      781 gtaccggctc gccctggcgc agctcccggc ccgggtcgac ctgcagcttg gtctgcttga
      841 agccctctgg cggcttggcg atgacagcga ggcgatccag cgggcacgcg gcatcgtcga
      901 gcagcagccc gaatgcgccc ctgccctcgt catcctggcc gatctggagc agcgtagcgg
      961 caacacgcgc cgcgccgagg atctcctcaa gcggctgcgc ggggttgatc cggatggcgc
     1021 aatcgtcgct ggaatgatcg ccctcaaccc acgttcgaat cagcagttcc tggcgctcgc
     1081 gccggccgcg atgccgtttg tggacgagca gctggccgcc gcggcacagg aacgacccaa
     1141 gatcgcgccg gcgcctgact tcgattaccg gccgacccgt agcgagattc cgacgcccga
     1201 tatcgacgaa ctggcgccga tgtcgttcga agagttcggc gcagccgacg cggaaagtcc
     1261 ggctgcgtcg ccggccgatg tcgcggcctt caacttcgac gagttcagcg atctgcccga
     1321 ggggctggag ccgatctcct ttgaagagct tggcgcgttg tctgacgagc aggctaccgg
     1381 tgaaagcgct gacttcgaga tggccgcctt cgatacggcc ccggaagccg aagcgccggc
     1441 gccgttcacg cttgaagaag ttctgcagtt cgaatcgccc gcggctgaga cgcctgcgga
     1501 cgatccccag cagctggttg cagatgatgc agcgttcatc gccgagccgg ccgcaacgat
     1561 gcaggagcag ccggccgccg gcatcgacga tctcgcctcg ctggcagccg ccctcgaagg
     1621 tgatgttgcc gatgccctga accgggccgg cgagccctac gacctggccg ctggcgttgg
     1681 ggagagccgc ccgccggccg cgaccccgcg tggatacacg accgtgctgc gcggccttga
     1741 agatcagggt ctggcgccgt tcgatccccg ctcgcgggag ccgatcgaag gcatgccctc
     1801 gatgctgccg gatgaggatc cgatctttga ggagccggca ccagccgcca gcgacgagag
     1861 cccggcccag accgatgagt tcggcaagat cacccgtgac tgggacacga tcgacgatga
     1921 gattcaggcc gcgctgccgg tccagccggg ccgtggctac accgaagaac tgcgcgccct
     1981 tgacgagatt ggcctgacgc cgttcgaggt cgaggacgat gaggatgcag tcgcgggcgt
     2041 gacgccgttc aatccgtttg ccagcgaaca gctggctgtc gatccgtcga tgcccgaacc
     2101 ggtcgtcgac gagcggccgg ccgccgtcgc tgagccggct ccggccgatg ccgccaccga
     2161 cgagctgctg ggcgggctgg agccgttcgc gttcgaagag ttcgacagcg accagagcac
     2221 ggccggcgat acgcagaccc gctccttctt caaccagggt ggctgggata gcagctcgtc
     2281 cgccgtcccg agcgatgccg atctcgacgc gctgcttgcc tatgaggacg aagagccgct
     2341 gccggtcgct gagccggagc ctgaaccggc gccagagccg gtcgcggcag ccgagcccga
     2401 gatcgcgctc gatgacgcat ccttcgccga tgcgatggcg tccgatcagg agccggccgc
     2461 cacggccagc gaagctgcca acctggcgac accaccggga ttgtccgagg cggatctgaa
     2521 cgagttcttc gagagttccg tcgccgtcac ccgccagctg cctggcgagg ccgaacatcc
     2581 gatgttccag gctgacgcgc cgctggcgga agccgtggca gcagagcagg cagccggaag
     2641 tgagcccgct gcgggcgaac cggccgagga gctgcagccg cagagcctga cctcggcact
     2701 gcgccccaac acggagatct tcacccgcgc gcgggccgtc aagctgggcc tgatgtccga
     2761 agggcgcatc aatggcaccc gcgagctggg cgaggggatc acgaccgacg agctgatggc
     2821 gatggaagag cgcctgaaga cgcgcaacct gccacagctc gacactgacg atttcgacga
     2881 cgcggatgac gaggacgatc tggtcgccgc gaccggcgcg acacgcgaca cgcgcacgct
     2941 gcgagctgcc cttgaggtga cacccgacga cgatgagcta cgctggtggc tggccgaggc
     3001 gctgcgcgac cggggtgatc ttgatgacgc gtattccgag taccgctggc tgatccgcca
     3061 cgctccgcac cggcacgacc agatcctcga tgccctcact gaatcggtcg accgggacca
     3121 gatgcccgag acagcccacc ggctgctggg cgatatctac cgccggcgcg gcgatgtcac
     3181 ccgtgccagc agtcacgccg cgctcgcatt gcagatgcgc cgccgtagtg gccgcgtcgt
     3241 ctagcagagc ggggtgggac gtggaacgga aatggaagcg aaggcaatgg tagacgagag
     3301 cccggccggc gggttgcgcg atcagttgat gcagatgctc gagtatcgag tcgtgcttgg
     3361 tgccgtcgtg acgacaatgg acggcctggt catcacatac gctggacttg gccccgacga
     3421 cgccgagatg ctggcggcgg cctccagctc gcaacccgac gacaaccagt attcgtccga
     3481 caagaccggt ggcggcacgc tccacgtcct gcgcgggaac gacgtgcgcc tgatcctgct
     3541 gaccgacagc gatgccccgc aaccggccgt caccgacctg atgagccagc acctcgggaa
     3601 cctcgaagac tcgctggccg tctagccccc tgtcgtccac cgcccagagg gcatccggca
     3661 gccgaaggac ctctccgcgt cagccgcttg ctgagtcaag gcggagaggt ccttcggctg
     3721 cggcctcagg acgacaggcg agcctgtaca ctcttcgctg atcgaaatga ccgatcgcga
     3781 ttggctgcat cagggaggac caagcgtggc gagcaacgac cgaacattga ttattgtcaa
     3841 gccggatggc gttcagcgcg ggctgatcgg ggagatcctc agtcggctgg agcggcgcgg
     3901 gctgaagttc gtcggcctga agcagatcac gatgagccgc gagctggccg aggagcacta
     3961 cgccggcctg aaggacaagc ccttctatcc caagctggtt gactacatca cctcggcgcc
     4021 ggttgtcgtc ggtgtcgtcg aagggccgaa cgccttccag accgtccgcg acacgatcgg
     4081 cgcgaccgac ccgctcaagg ctgcccccgg caccgtccgc ttcgagttcg gcatctcaat
     4141 cggccgcaac ctgagccacg cctccgacgg cgacgagagc gccgtccgcg agatcaacct
     4201 cttcttcagc cccgacgagc tctccagctg ggcccgcgac acggacgcgt ggattctgga
     4261 agagtaaggg attttcggga cgtgggacgt gggacgcggg acgcgggacg tgggacgcgg
     4321 gagtggctga cgccgcacgc gccccggacg gacggcgtga gtgaggaccg cacccctcac
     4381 cccggtcatc ctgagccgca gcgaaggatc gctcggctgt aacggctcgt gtggtggacg
     4441 aggaccgacg cgcgcggcta cc
//
LOCUS       DASUGJ010000621         6659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_518738, whole genome shotgun sequence.
ACCESSION   DASUGJ010000621 DASUGJ010000000
VERSION     DASUGJ010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518738"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..869)
                     /locus_tag="VFV93_14165"
     CDS             complement(207..869)
                     /locus_tag="VFV93_14165"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEX5166544.1"
                     /translation="MSNRTPDEPDRQAGDEFPLGPIGGYLSVVLRGLLVRFRFANLAL
                     RHESRWLLSLFSFINGCLSIAIMTTVALISGQPFIFPSLGPTAFLFFYTPLAPPASPR
                     NTIIGHLIGALAGYGSLLIFGLQDVGPALATGVTAERVGAAALSLGLTSGLMVLLKSP
                     HPPAGATTLIISLGILHTLDQIVVLMIAVILLTGQALIINRLAGLPYPLWSAPSAPPA
                     NR"
     gene            complement(874..1158)
                     /locus_tag="VFV93_14170"
     CDS             complement(874..1158)
                     /locus_tag="VFV93_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166545.1"
                     /translation="MVQPDRDPDRDPGARQTIAPERRPPPGQAGLALLGLAVGVTLIS
                     IQLWLLTLAFDLYKFDEDTDAILAAAFSGLVFFGGLAMLHILDRRPRRRG"
     gene            complement(1145..1681)
                     /locus_tag="VFV93_14175"
     CDS             complement(1145..1681)
                     /locus_tag="VFV93_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX5166546.1"
                     /translation="MSSSDDDTRLLSNRTRAGRWQVDFPYEWDADDLVSRRQVLRWAV
                     WASGALFAATAGLAGLTLARNQHRGGEQRIIESDAVPTGGVHYFHYPGKDDHAILLRL
                     TEERFVAYSGKCTHLSCAVYWDEDREELICPCHEGVFAPETGDVIAGPPPRPLPRIVV
                     TERDGAVYAIEEVPNGPA"
     gene            complement(1678..2202)
                     /locus_tag="VFV93_14180"
     CDS             complement(1678..2202)
                     /locus_tag="VFV93_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5166547.1"
                     /translation="MIMVDFIDRGMSVATQPTVCMHCQDPVAPCAQVCPVLAILISPE
                     GVVQQAEPSRCIGCRNCVYACPFGVPKFDIEARLMKKCNLCYDRTVQDLKPWCAQACP
                     TDALWYGDYEEFVNEREGRAVNVTAFGRQQVQTKVYHVLPDETPRLDVTLLIAEAEAQ
                     LALAGTTNEEAWVL"
     gene            complement(2438..4744)
                     /locus_tag="VFV93_14185"
     CDS             complement(2438..4744)
                     /locus_tag="VFV93_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX5166548.1"
                     /translation="MSVNKDIFVPTRDFNYDFQPAGRPMSGWTEAAQLPDSLVPTHCA
                     FCGVQCGMYLRVEDGKVTGVEPRDYAHNRGSLCPKGVVAYQQVNHPDRLRYPMIRRGG
                     KGGTLERATWDEAYDYIISRWQRIQAEHGRDAVAVYSGSSMTNEKCYLAGKFARVGLR
                     TRNIDYNGRLCMSSAAVGYARAFGIDRAPLPMTDIPLTKSIMVAGSNVAECFPIVMQW
                     IWRARDRGASLIVIDPRETPLARTADLWLPVRPGTDSALLNAILRVIIHDGLADEAYL
                     RERTNGWDEVRAAVEPFTPEYAEKITGVPAARIVAAARLYGQADPSLIMHARGIEHSS
                     HGVDNVLACTNLALARGMVGKPGGGTMMITGQGNGQGGREHGQKANQLPGYRHIDVPE
                     ERQQMAEFWGVPEEEIPWEGAAATEMMHLMAEGDIRSCIVICSNLMVSLPDNAVVQRA
                     LQNLDPLIVVDFFMSETAELADVVLPGTVWCEDEGTTTNLEGRVVKINPAAPPPGEAR
                     NDWIIMTELAHRLGRGQFFPYTSAREIWDELRAASRGTVADYYGITWERIEREQGVFW
                     PCPSTDHPGSPRLFTERFGHADGKAKMFPIEYRPPAEEPSRDFPFRLTTGRVVYHYLS
                     GNQTRRLGFLNSQAPEPWVEIHPQAAAPLGISDGQVVRVRTARGSMEVKALVVPTIRP
                     DTIFIPYHWGHRQAANQLTSPAVDPGVKIPEYKACAAAIERLDAPIPAGGTPTINFTA
                     ETFPKMFPYEVVEPKQGGTSDVAGTARH"
     gene            complement(4876..5958)
                     /locus_tag="VFV93_14190"
     CDS             complement(4876..5958)
                     /locus_tag="VFV93_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166549.1"
                     /translation="MEHARRRAGIATVVVTLILLVLIYVGSRGLKHFDSALIGYCVAS
                     IFAIAALTHRYTLWLGRAPTWRYFRAGWANFFSWKNFRRYTFLIPKAWWTDIFAQTFI
                     FKRSPTRWVMHMSIFWGVLLSLAVTFPLTFGWIHFELAENNPDHYTAFFFGFAIFTFP
                     IEAGVGFGVYHILDFTAVLLIVGLAIAIWRRITDAGLLTTQRFGFDLLPLFLLFAIAV
                     TGLALTASSMWWNGAFYWFIALVHQVVVVLWLLSLPFGKFFHIVQRPASIGVSLYQAV
                     NQGVRHYGGRPVEADPTVIGTGACRRCGDPLPSPQFIADLKGVLRDVGQDYALNDDTA
                     LQDYCPTCKRVLRGQSYYAVMGKKFL"
     gene            6125..6490
                     /locus_tag="VFV93_14195"
     CDS             6125..6490
                     /locus_tag="VFV93_14195"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5166550.1"
                     /translation="MAQVFRLERRWQEHGLPKRQVQYGITSLAPDSGTPATLLALKRG
                     HWGIEHGLHRVKDVTFGEDASLIHCGSGPLVMALFRDTAISLLYRTGIRQVAACLRRF
                     SQQPMQALDLVLCSPATHA"
BASE COUNT         1149 a         2052 c         2372 g         1086 t
ORIGIN      
        1 agacacgcaa gcgccaatcg gtagaacgag tttggccctt ccgaacctct cgtgtcactg
       61 gcgccaggcg atccttcgct gcggctcagg atgacagggg gagagggtgc ggtcctcggg
      121 agcgtcgtac ggctggtccg cgtcacgcat cacggcccct gtcgatgaca cgcacggcga
      181 tgggttgcga gcgtttccgt tgtcgatcag cggttcgctg gcggggctga cggtgccgac
      241 cagagcggat acggcaggcc cgccaggcgg ttgatgatca gcgcctggcc ggtcaggagg
      301 atgaccgcga tcatcaggac gacgatctgg tcgagcgtgt gcaaaatgcc gagcgagatg
      361 atcagcgtgg tcgccccggc cggtggatgg ggcgacttca gcaacaccat caggccgctg
      421 gtgagtccga gcgacagcgc tgccgcgccg actcgttcag ccgtcacgcc ggtcgccagc
      481 gctggtccga cgtcctggag tccgaagatg agcaggctgc cgtagccggc cagcgcgccg
      541 atcaggtggc cgatgatcgt gttgcgcggg ctggcaggtg gtgcgagcgg cgtatagaag
      601 aagaggaacg ccgtcggtcc cagcgaaggg aagatgaacg gctggccgct gatcagcgcg
      661 accgtcgtca tgatcgcgat cgacaggcag ccgttgatga agctgaagag gctgagtagc
      721 cagcgcgatt cgtggcgcag ggccaggttg gcgaaccgga accgcaccag caacccacgc
      781 aggacgaccg acaggtagcc gccgattggt cccagtggaa actcatcacc agcctgccga
      841 tcgggctcat cgggcgtcct gttggacatg cgctcaccca cggcggcggg ggcgacggtc
      901 gaggatgtgg agcatggcga ggccgccgaa gaagacgagg ccggagaagg cggcggccag
      961 gatggcatcg gtgtcctcat cgaacttgta gaggtcgaag gccagtgtca gcagccagag
     1021 ctggatgctg atgagggtga cgccgacggc caggccgagc agggccagcc cggcctggcc
     1081 ggggggcggg cggcgttcgg gcgcgatcgt ctggcgggcg ccggggtcgc ggtcggggtc
     1141 acggtcaggc tggaccattc gggacctcct cgatggcgta gacggcgccg tcgcgctcgg
     1201 tgacgacgat ccgggggaga gggcgcggcg gcgggccggc gatgacatca ccagtctcgg
     1261 gcgcgaagac gccttcgtgg caggggcaga tcagctcctc ccgatcctcg tcccagtaga
     1321 cggcgcagga gaggtgtgtg cacttgccgc tgtaggcgac gaagcgctcc tcggtcagcc
     1381 gcagcaggat ggcgtggtcg tccttaccgg ggtagtggaa gtagtggacg ccgccggtcg
     1441 ggaccgcgtc ggactcgatg atccgctgct cgccgccccg gtgctggttg cgggccaggg
     1501 tcagcccggc caggccggcc gtcgcggcga agagcgcgcc ggaggcccag acggcccagc
     1561 gtagcacctg gcggcgggag acgaggtcgt cggcgtccca ctcgtagggg aaatcgacct
     1621 gccagcggcc ggcgcgggtg cggttgctca gcaagcgggt atcgtcgtcg gacgagctca
     1681 taggacccat gcctcctcgt tggtggtacc ggcgagcgcg agctgtgctt cggcctcggc
     1741 gatcaggagc gtcacatcca gccgtggcgt ctcatccggc aggacatgat agaccttcgt
     1801 ctgcacctgc tggcggccaa aggcggtgac gttgacggcg cgaccttcgc gctcgttgac
     1861 gaactcctcg tagtcgccat accagagggc gtcggtcggg caggcctgcg cgcaccaggg
     1921 cttgaggtcc tggaccgtgc ggtcgtagca gaggttgcac ttcttcatca gccgggcttc
     1981 gatgtcgaac ttcgggacgc cgaacgggca ggcgtagacg cagttgcggc agccgatgca
     2041 gcgcgacggc tcggcctgct ggacaacacc ctcgggcgag atcaggatcg cgaggaccgg
     2101 gcagacctgg gcgcagggcg cgaccgggtc ctgacagtgc atgcagacgg tcggctgggt
     2161 ggcgacgctc atgccccggt cgatgaaatc gaccatgatc atgctctcgc ccttgtgcga
     2221 atcacactcg cgacaagccg cctcgcacgc ccggcacccg atacagcgcg tcggatcaac
     2281 gaagagggtt ttgatggcca tgggggtcct ttcggtgggt cgggaatcgg gaatcgggat
     2341 tcggggctgg gtagcgccgt tcgctctttg tcggaggtat ctgacgtaca aggatcatgt
     2401 gacgtacctc agccccgaat cccgattccc gaccccatca atgcctcgcc gtcccggcaa
     2461 catccgacgt tccgccctgt ttcggttcga cgacctcgta ggggaacatc ttggggaacg
     2521 tttcggcggt gaagttgatg gttggggtgc cgccggccgg gatcggggcg tcgaggcgtt
     2581 cgatggcggc ggcgcaggct ttgtactcgg ggatcttgac gccgggatcg acggccgggc
     2641 tggtgagctg gttggcggcc tggcgatggc cccagtggta ggggatgaag atggtgtcgg
     2701 gccggatggt cgggacgacc agcgccttga cctccatcga gccacgtgcc gttcgaacgc
     2761 ggacgacctg gccatcgctg atgccgagcg gtgcagcggc ctgcgggtgg atctcgaccc
     2821 acggttccgg tgcctgcgag ttcaggaagc cgagccggcg cgtctggttg ccggagaggt
     2881 agtggtagac gacgcggccg gtcgtcagcc gaaaggggaa gtcgcggctg ggctcctcgg
     2941 ccggcgggcg atactcgatc gggaacatct tggccttgcc gtcggcgtgg ccgaagcgct
     3001 cggtgaagag gcgcggcgag ccggggtggt cggtactcgg gcagggccag aagacaccct
     3061 gctcccgctc gatccgctcc caggtgatgc cgtagtagtc ggcgacggtc ccccgtgagg
     3121 cagcacgcag ctcgtcccag atctcgcggg cggaggtgta ggggaagaac tggccgcgac
     3181 ccagccggtg ggccagctcg gtcatgatga tccagtcgtt gcgggcttcg ccgggcggtg
     3241 gcgcggccgg gttgatcttg acgacgcgcc cttcgaggtt ggtcgtcgtg ccctcgtcct
     3301 cgcaccagac cgtgccgggc aggacgacat cggccagctc ggccgtctcc gacatgaaga
     3361 aatcgacgac gatcagcggg tcgagattct ggagcgcgcg ctggacgacg gcgttgtccg
     3421 gcagcgagac catcaggttc gagcagatca cgatgcagga gcggatgtcg ccctcggcca
     3481 tcaggtgcat catctcggtc gcggcggcgc cctcccaggg gatctcctct tccgggacgc
     3541 cccagaactc ggccatctgc tggcgttcct ccggcacatc gatgtggcgg tagcctggca
     3601 actggttggc cttctggccg tgctcgcgcc cgccctggcc gttgccctgg ccggtgatca
     3661 tcatcgtccc gccgcccggc ttgccgacca tcccgcgcgc cagcgcgagg ttcgtgcagg
     3721 cgaggacgtt atcgacaccg tgcgagctgt gctcgatccc gcgtgcatgc atgatgagcg
     3781 acgggtcggc ctggccatag aggcgggcgg cggcgacgat ccgcgcggcc gggacgccgg
     3841 tgatcttctc ggcatactcc ggcgtgaacg gctcgacggc ggcccgaacc tcgtcccagc
     3901 cgttggtcct ctcccgcaga taggcctcat ccgccagccc gtcgtggatg atgacgcgca
     3961 ggatcgcgtt cagcagcgcg ctatcggtgc cggggcggac cggcagccag aggtcggcgg
     4021 tccgggccag cggcgtctcg cgtgggtcga tcacgatcag gctggcgccc cggtcgcggg
     4081 cgcgccagat ccactgcatc acgatcggga agcactcggc gacgttggag ccggcgacca
     4141 tgatcgactt cgtcagcgga atgtcggtca tcggcagcgg agcgcggtcg atcccgaagg
     4201 cccgcgcata gccaaccgcc gccgaggaca tgcagagacg gccgttgtag tcgatgttgc
     4261 gcgttcgcag gccgacccgg gcgaacttgc cggccaggta gcatttctca ttcgtcatcg
     4321 acgaaccgct gtagacggcg accgcgtcgc ggccatgctc ggcctggatg cgttgccagc
     4381 gggagatgat gtagtcgtag gcttcgtccc aggtggcccg ttcgagcgtg ccgcccttgc
     4441 caccgcgccg gatcatcggg tagcgcagcc ggtcggggtg gttgacctgc tggtaggcga
     4501 cgaccccctt cggacagagt gagccgcggt tgtgggcgta gtcacgcggc tcgacgcccg
     4561 ttaccttgcc atcctcgacg cgcaggtaca tcccgcattg gaccccgcag aacgcgcagt
     4621 gggtcggaac cagcgaatcc ggcaactggg ccgcctcggt ccagccactc atcggccgtc
     4681 cggccggctg gaagtcgtag ttgaagtccc gtgtcgggac gaaaatgtct ttgttgaccg
     4741 acaaggctta ctccgtttcg cgcgggattc gggattcggg gatcgggagt gccgcacact
     4801 ctttgtactg accatgacgc cgatattcag cgattggcgc tacccattct cgaatcccga
     4861 atcccgaatc ccgacctaca agaacttctt ccccatcacc gcgtaatatg actgaccccg
     4921 caggacgcgt ttgcaggttg ggcagtagtc ttggagggcg gtgtcgtcgt tgagggcgta
     4981 gtcctggcca acgtcgcgca ggacgccttt gaggtcggcg atgaactgcg gcgagggcag
     5041 cgggtcccca caacggcggc aggcgccggt gccgatcacg gtcgggtcgg cctcgaccgg
     5101 tcggccgccg tagtggcgga cgccctggtt gacggcctga tagagcgaga cgccgatgct
     5161 ggccgggcgc tggacgatgt gaaagaactt gccgaacggt agcgagagca gccagaggac
     5221 gacgacaacc tgatggacga gggcgatgaa ccagtagaac gcgccattcc accacatcga
     5281 cgaagccgtc aatgccagcc cggtgacagc gatcgcgaac agcaggaaca gcggcagcag
     5341 gtcgaagccg aagcgctgag tcgtcagcaa cccggcatcg gtgatccgtc gccagatggc
     5401 gatcgcgagg ccgacgatca gcagaacggc cgtgaagtcg agaatatggt agacgccgaa
     5461 cccgacaccg gcctcaattg gaaaggtgaa gatggcgaag ccgaagaaga aggcagtgta
     5521 gtggtcgggg ttgttctcgg ccagctcgaa gtggatccag ccgaaggtga gtgggaaggt
     5581 gacggccagc gagagtagca cgccccagaa gatcgacatg tgcatgaccc agcgggtcgg
     5641 gctgcgcttg aagatgaagg tctgggcgaa gatgtcggtc caccacgcct tcgggatcag
     5701 gaaggtatag cggcggaagt tcttccacga gaagaagttg gcccaaccgg cccggaagta
     5761 gcgccaggtc ggcgcccggc cgagccagag cgtgtagcgg tgggtgaggg ccgcgatcgc
     5821 gaagatcgac gcgacgcagt agccgatcag ggccgagtcg aaatgcttca acccgcgtga
     5881 cccgacgtag atcaacacga gcaggatcag ggtgacaacg accgttgcaa tcccagcccg
     5941 ccgccgtgcg tgctccatgc ccaaaccccc gtcgaactaa ccagacggat ccctgtcgca
     6001 gttggtgcgg caagaaccac gccaccggta gcgtagcatg gacagggacg gtcgtgggca
     6061 acagcggagt ctcagacgat catggcccgt cacacaaacg accggctacg tcgagtggcc
     6121 gggattggcc caggtctttc ggctggagcg ccgttggcag gaacacggcc tgcccaagcg
     6181 gcaggtccag tacggcatca ccagtctggc acccgacagc ggcacgccag cgacgctgct
     6241 ggccctcaag cgcgggcatt ggggcatcga gcatgggctc catcgggtca aggatgtcac
     6301 ctttggcgag gacgccagtc tgatccattg tggctccggg ccgctggtga tggcgctctt
     6361 ccgcgacacc gccatcagtc tgctgtaccg caccggtatc cggcaggttg ccgcctgcct
     6421 ccggcgattc agtcaacagc caatgcaggc cctcgatctc gtcctgtgct caccagcaac
     6481 tcacgcataa gccctgtatt caagggtggc cccacgcaag gtggaggtca ccgcatggtc
     6541 tctgccggag accgtaccat cgtctatgcc ccggcggtca taggcctggt agcgctggtg
     6601 acctcacgtg ttgcagtgga atccgtcgac ggatggcccg tcgctgccgg tccgcgggc
//
LOCUS       DASUGJ010000622         4630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1073831, whole genome shotgun sequence.
ACCESSION   DASUGJ010000622 DASUGJ010000000
VERSION     DASUGJ010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073831"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFV93_14200"
     CDS             complement(<1..627)
                     /locus_tag="VFV93_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5166551.1"
                     /translation="MATTKQQAASSKIAHSPGIYADVVEAIGRTPLVRLDRVNRGNPA
                     TLVAKLEMLNPGGSVKDRIGIRMIEEAERKGWIKPGGTIVEPTSGNTGVGLAMAAAIK
                     GYRCVFVMPDKVSGEKIALLRAYGAEVVTTPTNVERESPESYYSVADRLTRELPNAFQ
                     PNQYFNPMNPRTHYESTGPEIWQGTDGKVTHFVAGVGTGGTISGISKFL"
     gene            complement(838..1320)
                     /locus_tag="VFV93_14205"
     CDS             complement(838..1320)
                     /locus_tag="VFV93_14205"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5166552.1"
                     /translation="MDLLDRQLAHDEWATTQLLNRSRDLTDAQLDQEFDIGHRTLRET
                     LDHLIFIVDFWTTLMAGQPVKWERNVHRSIGEMADRHARFYPAFATLARRIHDEQRLD
                     DTFTDHFGGPMTYGGAISHVILHDAEHRVEAVHMLGRLGLDNVPEVDHGLWDYERRGL
                     "
     gene            1572..3002
                     /locus_tag="VFV93_14210"
     CDS             1572..3002
                     /locus_tag="VFV93_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166553.1"
                     /translation="MAVAGDRRGSLIWFALLVALVLVACGEDEDAPAAAATDAATASP
                     PTATAGGSLTVSPVSATPNAIPTGPAPATTTITESVDDAATPLVVWADTAQLDNPTLG
                     VDVYWLGEQFDPAGDLPLLVLTWVTGSPQPGGGPPGMRAILEYPTASDEPGGVNLMLV
                     DRAEWEAVLARDPATDDYARLARMFWDSPCATQETVELDGGQATIYASYQALAGPSTG
                     DCPAGQFDRYLAHAVFGETVVLVNAPLSLQQPPDVPDQPYDSLEGMRQVVLGLRLREP
                     VLDPTPVPTPAASLDDPGSLVLQPADLPGRYGYGDDGCSPRYECNRGPTGFGSEGGYG
                     RLVEAAGQFLGFYYQYEHVGFNSSGPPDPNIEPPVIDSFALVCLQSCDPAAILEAGQE
                     LLRYNGSSDSVSLDGPPALGDETRLYRVDTLVFGQITRGYAVIWRHGSVVGMVIVGGA
                     DEVAGRQLAIDLATKQAERIAFAVGR"
     gene            3058..3798
                     /locus_tag="VFV93_14215"
     CDS             3058..3798
                     /locus_tag="VFV93_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5166554.1"
                     /translation="MATFVLVHGATVGGWCWRWVERELRAAGHEVHTPTMTGLGERAH
                     LATPEVDLDTHINDIVNVMTYEDLRDVVLVGWSYGGMAVAGVADRLPERVVHVVYLDS
                     DIPRDGDTSVPPSRHAAREELARAFDGWRVPLGIDVDTTPPVAAWATWLELPREMRAW
                     IAERIVPHPLKTWTQPIRLTGAAASIPTTYIRCTIGHDPTDEDTARQDDRIRSEPTWR
                     YREIAESHLCPWTAPGAVVELLVESIAN"
     gene            complement(3795..4205)
                     /locus_tag="VFV93_14220"
     CDS             complement(3795..4205)
                     /locus_tag="VFV93_14220"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5166555.1"
                     /translation="MSEANKQIVRRFIDEVWNQARLEVADELVHPLYENNEGAGPEAV
                     KRNVRAFKSAFPDLHDSIEALIAEGDFVAARVTRSGTHLGTFRDVYEASGRAMSITEL
                     CLWRIEDGKLREGWFAAHEISLRKQLGVIPESLR"
     gene            4444..>4630
                     /locus_tag="VFV93_14225"
     CDS             4444..>4630
                     /locus_tag="VFV93_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166556.1"
                     /translation="MVQTTVLTGTMLTSFERHLRATNRAPKTVTLYLSAIRSFWTYLD
                     TLGYPRDIAAVSREHVEL"
BASE COUNT          773 a         1528 c         1444 g          885 t
ORIGIN      
        1 gaggaacttc gagatgcccg agatcgtccc gccggtcccg acgccggcca cgaagtgggt
       61 gaccttgccg tcggtgccct gccagatctc tggtccggtc gattcgtagt gggtgcgtgg
      121 gttcatcggg ttgaagtact ggttcggctg gaaggcgttg ggtagctcgc gcgtcagccg
      181 gtcggcgacc gagtagtacg actccggcga ctctcgctcg acgttggtcg gtgtcgtcac
      241 gacctcggct ccgtaggcgc gcaggagcgc gatcttctcg ccagagacct tgtcgggcat
      301 gacaaacacg cagcggtagc ccttgatcgc cgccgccatc gccagcccga cgccggtgtt
      361 gccggaggtc ggctcgacga tcgtcccgcc cggcttgatc cagcccttgc gctcggcctc
      421 ctcgatcatc cgaatcccga tccggtcctt gaccgaacca cccggattca gcatctccag
      481 cttggcgacc agcgtcgccg ggttgccgcg attgacccgg tcgagccgca ccagcggcgt
      541 ccgtccaatt gcctcaacga catctgcata gatccccggg gaatgcgcga tcttgctcga
      601 cgctgcctgc tgcttcgtgg ttgccatcta cgcggctcgg cgctgagccg ccccccttcc
      661 agatacacgc tgcacgggtg gacgacgggc cacatcccgc cgctgattgc acttggattg
      721 tagcaatgat ctccgcttcg gcatgcgcgc tgctcagacg tagccactga ccatgcaacc
      781 gcgtccctcg atagcttcgc gattgcattg gtgtattgcc gttcggcagc tggttgacta
      841 caacccgcgc cgctcgtagt cccagagccc atggtcgact tcgggcacgt tgtccagtcc
      901 caacctccca agcatgtgaa ccgcctcgac ccggtgctca gcgtcgtgga ggatgacgtg
      961 gctgatcgcg ccgccgtagg tcatcgggcc gccgaagtgg tcggtgaagg tgtcatcgag
     1021 gcgttgctcg tcgtggatgc ggcgggcgag ggtggcgaag gccgggtaga agcgggcgtg
     1081 gcggtcggcc atctcgccga tcgagcggtg gacgttgcgc tcccatttca ccggctgacc
     1141 ggccatcagc gtcgtccaga agtcgacgat gaagatcagg tggtcgagcg tttcgcgcag
     1201 cgttcggtgg ccgatgtcga actcctggtc gagctgcgcg tcggtcaggt cgcggctgcg
     1261 attcagcagc tgcgtcgtgg cccattcgtc gtgcgccagt tgccggtcga gcaggtccaa
     1321 tcttcctcct ccggaatcgt gcgtgccgct cgcaggttcg atatctactg atgataatgc
     1381 gatactcgcc ggctcgcgtc gtatgtcgtg ttcatcgaat tcgctgatcg cctgacgaag
     1441 cgtcgaatca ctccgcgatt ccgaatgtga acaggtatac ttgtctgaga caagcttgac
     1501 tgttgtgcaa tcgccggtcg tcgcttcacg cgtatcgact ggccgagtga gcgagcctga
     1561 atcggggaga gatggcagtt gccggtgatc gccgtggatc gctgatctgg ttcgctctgc
     1621 tcgtcgcgct cgttctcgtg gcctgtggcg aggatgaaga cgccccggct gcggcggcga
     1681 cggatgccgc gacagcgtcg cctccgaccg cgaccgctgg cggttcgctg acggtctcgc
     1741 cggtctcagc gacgccgaac gcgattccaa caggccccgc tccggcgacc acgactatca
     1801 ccgaatccgt cgacgatgcc gcgacgccgc tcgtggtctg ggctgatacg gcacaactcg
     1861 acaaccccac cctcggcgtc gatgtctact ggcttggcga acaattcgac ccggctggcg
     1921 atctgccgtt gctggtgctt acgtgggtta cgggctctcc tcagcccggc ggtggaccgc
     1981 ctggcatgcg cgccatactt gaatacccga ccgcctcaga tgaacctggc ggcgtgaatc
     2041 tcatgctcgt ggaccgcgcc gagtgggaag ccgtgctcgc tcgcgatccg gcgaccgacg
     2101 actacgccag gctggcccgg atgttctggg attcgccctg cgcgacgcag gagacggttg
     2161 agctggacgg cggccaggcc accatctacg ccagctacca ggcgctggca ggcccatcaa
     2221 caggcgactg tcctgccgga cagttcgacc gctatctcgc ccacgccgtc ttcggtgaga
     2281 ccgtcgtcct cgtcaacgcg ccgcttagct tgcaacaacc accagatgtg ccggaccaac
     2341 cgtacgactc gctggagggc atgcgtcagg tcgtgctcgg cctgcgactg cgcgagccgg
     2401 tcctggaccc aacgccggtt ccgacgccgg ccgccagcct cgatgacccc ggctcgctcg
     2461 tcctccagcc cgccgacctg cccggacgat atggctacgg cgacgacgga tgctcgcccc
     2521 gctacgaatg caacagaggc ccgaccggct tcggttccga gggcggttac gggaggctgg
     2581 tggaggccgc cggccagttc ctgggcttct actaccagta cgagcacgtc ggcttcaaca
     2641 gtagcggacc acccgacccg aacattgagc cgccggtgat cgacagcttc gcgctcgtct
     2701 gcctccagtc ttgcgatccg gccgcgatcc tcgaggccgg ccaggaactc ctgcgctaca
     2761 acggcagcag cgattcggtc tcgctcgacg gaccgccagc cctcggcgac gagacacgcc
     2821 tctatcgcgt cgacacgctc gtcttcggcc agatcacgcg gggctacgcc gtcatctggc
     2881 ggcatgggtc ggtcgtcggg atggttattg tcggcggggc ggatgaggtc gccggtcgcc
     2941 agctcgccat cgatctggcg acgaagcagg ccgagcgcat tgcattcgcg gttggacgat
     3001 aattctctcc atcggtgcaa cgaaacattg cagcgtcagt tcgagaggag tccatcgatg
     3061 gctaccttcg ttctcgtcca tggcgccacg gttggcggct ggtgctggcg ttgggtcgag
     3121 cgggagttgc gcgcagccgg ccacgaggtc cacaccccga cgatgaccgg cctcggcgaa
     3181 cgggcccatc tcgcgacgcc ggaggtcgat ctcgacaccc atatcaatga catcgtcaac
     3241 gtcatgacgt acgaggacct gcgcgacgtc gtcctggttg gctggagcta cggcgggatg
     3301 gctgtcgccg gtgtcgccga ccgattgccg gagcgagtcg ttcacgtcgt ttacctcgac
     3361 tccgacatcc cgcgtgacgg cgacaccagc gtcccgccga gccgccatgc cgcccgcgag
     3421 gaactggccc gtgccttcga tggctggcgc gtcccgctcg gcatcgatgt cgacacgacc
     3481 ccacccgtcg ccgcctgggc gacgtggctg gagctgccgc gcgagatgcg cgcctggatc
     3541 gccgagcgga tcgtcccgca cccgctcaag acctggaccc agccgatccg gctgaccggc
     3601 gcagccgcct cgatcccaac cacctacatc cgctgcacga tcggccacga cccgaccgac
     3661 gaagacaccg cgcggcagga cgaccgtatc cgcagtgaac cgacgtggcg ctatcgtgag
     3721 atcgcggaga gccatctctg cccctggact gccccgggcg cggtcgtcga actcctggtc
     3781 gagtcgatcg caaactagcg aaggctctcc gggatgacac cgagttgctt ccggagactg
     3841 atctcatggg cggcgaacca gccttcgcgg agcttgccgt cttcgattcg ccagagacac
     3901 aattcggtga tcgacatggc tcgaccggaa gcttcataga cgtcacgaaa ggtgccaagg
     3961 tgggttccgc tccgcgtgac gcgagcggcg acgaagtcac cttcggcgat caacgcttca
     4021 atgctgtcat gcaggtcagg gaatgcagac ttgaacgctc gcacgttgcg cttgaccgct
     4081 tccggccccg ctccttcgtt gttctcatac aacgggtgga ccagttcgtc agcaacctcc
     4141 agcctcgcct gattccagac ttcgtcgatg aacctccgga cgatctgctt gtttgcttcg
     4201 ctcatgtgtg gtcaccaatc tgtgggcggc ggttggctgc gacaggccgg tgttgaacgc
     4261 ggtgacgaca gtctagcgga tgctgggaat gtcgaatggt agggataacc ctaccttatc
     4321 gcttcttcgc ctgtacctga aaagcctatt cccgttggaa aaacaatctc cccctttcct
     4381 gttccagcgc tcgtagttat actaagtcaa gagacaaatt ttgactaaca ggaaggaacg
     4441 aaagtggttc agacgacggt tctcaccggg acgatgttga cctcgtttga gcgtcaccta
     4501 cgggcgacaa accgcgcgcc gaagacggtg acactctacc tgagcgccat ccgcagcttc
     4561 tggacgtacc tcgatacgct gggctatccg cgcgatatcg cggccgtgag tcgcgaacac
     4621 gtcgaactct
//
LOCUS       DASUGJ010000623         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1631345, whole genome shotgun sequence.
ACCESSION   DASUGJ010000623 DASUGJ010000000
VERSION     DASUGJ010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631345"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..2256)
                     /locus_tag="VFV93_14230"
     CDS             complement(91..2256)
                     /locus_tag="VFV93_14230"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5166557.1"
                     /translation="MVVLSGSLPSPLTSEDDFTNTPEAVKGSDLIEQRLHGKEPLTET
                     VIISSETQTVDDPAFQQVVIDTTSALRGMPDVVKSAGNYYEALDAGEPAAGQLVSDDR
                     MSTIIPVTLLGEYDDVSDYGTDYVAMIEAQGADGIDVYSVGTLSGGEIYGKIAEEDLG
                     SAEMFGLPIALLILVVVFGALVAAGVPLVVALSSIAVAMGISTIIGQIHPLSDIVMNI
                     IVMIGLAVGIDYALFIVERYREERRHGAAKHDAIAIAGGTATKAVVFSGMTVVIALAG
                     MFILPISVFRSLGIGAMIAVVVSVAASMTMIPALLALLGDKIDWPRRRKYDTLTAEQI
                     AANRFHDDSIHGGFWGRITKTVMARPVISAVLAIGILVAAALPYLDLKTGQTGIESLP
                     ESEVKSGYEILSEKFYAGILSPTEIVIDGNANSAEVQQGIDALVTALGNDPQYGPTAI
                     EVNEAGDLTLVSVPMTIDSSTPEAEQKIDDLRDTTIPNAFASADAEVYVTGDAAFNRD
                     FNLTMRTWTPIVFAFVLGLSFILLMLAFRSIIVPIKAIIMNLLSVGAAYGLLTLVFQK
                     GIGNEIFGFSQTPVIATWIPIFLFCILFGLSMDYHVFLLSRIREHYDSTHNNRESVAV
                     GLQSTAKIITGAALIMVAVFSAFAAGRLVEMQQMGFGLAVAVFLDATIVRSVLVPSTM
                     ALLGDRNWYLPRWLSWLPDLRVEGQARAQAPVAATAGDD"
     gene            complement(2518..2925)
                     /locus_tag="VFV93_14235"
     CDS             complement(2518..2925)
                     /locus_tag="VFV93_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166558.1"
                     /translation="MRHNGVIGTGWDFNRVIRLLLVDDQPQVRRGLMMRLALEPDVEI
                     VGEAGDAESALELAGRLQPDVVVMDVELPGMDGIDATRNLRTSLPQTAIVMLSLHDDI
                     ETIARATAAGAAAFIAKHRMEGPLLAAIRAAAM"
     gene            3286..>3693
                     /locus_tag="VFV93_14240"
     CDS             3286..>3693
                     /locus_tag="VFV93_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5166559.1"
                     /translation="MDSVSITIDAVEYERLRESQRVLLTLFENLPVMIYRCNNDADWS
                     MEFVSEGCRGLTGYDPAALTSGRVTYASIIQPADRDRVWDGVQAALQARSSFSLTYCI
                     TTASGEERWVWEQGQGVFDSHGELQGIEGFIIDV"
BASE COUNT          645 a         1166 c         1209 g          673 t
ORIGIN      
        1 ccatgtcacc atcgctcgcc ggtggcgggc aacgcaaaac cggccgggag ggtgatccgg
       61 ccggtttcgc gtacatggtt gcgttgaggg ctagtcgtcg ccggccgtcg cggcgaccgg
      121 ggcctgcgcg cgtgcctggc cttcgacgcg caggtcgggc agccaggaga gccagcgcgg
      181 caggtaccag ttgcggtcgc cgagcagcgc catcgttgac ggcacgagga ccgagcggac
      241 gatcgtcgca tcgaggaaga cggccaccgc caggccgaag cccatctgct gcatctcgac
      301 cagccggccg gccgcgaagg cggagaagac cgccaccatg atcagcgccg cgccggtgat
      361 gatcttggcc gtgctctgca ggccgaccgc gaccgactcg cggttgttgt gggtgctgtc
      421 gtagtgctcg cgaatgcggc tcagcaggaa gacgtggtag tccatgctca ggccgaacag
      481 gatgcagaac aggaagatcg gaatccaggt cgcgatcacc ggcgtctggc tgaagccgaa
      541 gatctcgttg ccgatgccct tctggaagac cagcgtcagc agaccgtagg ccgcgccgac
      601 cgagagcagg ttcatgatga tcgccttgat cggcacgatg atcgagcgga aggccagcat
      661 cagcaggatg aagctgagac cgaggacgaa ggcgaacacg atcggtgtcc aggttcgcat
      721 cgtcaggttg aagtcacggt tgaaggccgc gtcgccggtc acgtagacct ctgcatcagc
      781 cgaggcgaac gcattcggga tcgtcgtgtc gcgcaggtcg tcgatcttct gctcggcctc
      841 cggcgtcgag ctgtcgatcg tcatcggaac cgagacgagc gtcaggtcgc cggcctcgtt
      901 gacctcgatc gcggtcgggc cgtactgcgg gtcgttgccg agcgccgtca ccagcgcgtc
      961 gatgccttgc tggacctcgg cgctgttggc gttgccatcg atgacgatct cggtcggcga
     1021 gaggatgccg gcgtagaact tctcgctcag gatctcgtag ccgctcttga cctccgactc
     1081 cggcagcgac tcgatgccgg tctgtcccgt cttcaggtca aggtacggca acgcggcggc
     1141 gacgaggatg ccgatcgcga ggacggcgct gatgaccggg cgggccatga ccgtcttcgt
     1201 gatccggccc cagaagccgc cgtggatcga gtcgtcgtgg aagcggttgg cggcgatctg
     1261 ctcggccgtc agcgtgtcgt acttgcggcg gcgcggccag tcgatcttgt cgcccagcag
     1321 cgccagcagg gccgggatca tcgtcatcga ggcagcgact gagaccacga cggcgatcat
     1381 cgcgccaatg ccgaggctgc ggaagaccga gatcggcagg atgaacatac cagccagcgc
     1441 gataacgacc gtcatgcccg agaagacgac tgccttggtg gccgttccgc cggcgatcgc
     1501 gatggcgtca tgcttggcgg cgccgtggcg gcgctcctcg cggtagcgct cgacgatgaa
     1561 cagcgcgtag tcgataccga ccgccaggcc gatcatgacg atgatgttca tgacgatgtc
     1621 ggagagcggg tggatctggc cgatgatcgt gctgatcccc atcgcgaccg cgatcgagga
     1681 gagcgcgacg accagcggca ccccggccgc gaccagtgcg ccgaagacga ccaccagaat
     1741 gaggagcgcg atcggcaggc cgaacatctc ggcgctgccg aggtcttcct cggcgatctt
     1801 gccgtagatc tcgccgccgc tcagggtgcc gaccgagtag acgtcgatgc cgtcggcgcc
     1861 ctgtgcctcg atcatcgcca cgtagtcggt gccgtaatcc gagacatcgt cgtactcgcc
     1921 cagcagcgtg accgggatga tcgtcgacat ccggtcgtct gagacgagct gtcccgccgc
     1981 tggttcgccg gcgtccaggg cctcgtagta gttgccggcc gacttgacga catcgggcat
     2041 cccgcgcaga gcgctcgtcg tgtcgatcac gacctgctgg aaggccgggt catcgaccgt
     2101 ctgtgtctcg gacgagatga tcaccgtctc ggtcagcggc tctttgccgt gcagccgttg
     2161 ctcgatcagg tctgatccct tgacggcctc gggcgtgttg gtgaagtcgt cctcgctcgt
     2221 cagcggactg ggcaggctgc ccgacaggac gaccataacg atgatcaaca gtacccatgc
     2281 gcccagcgtt cgccatggcc ggcgagcgct tgcgcgcgca atccccgtcg tcgaaaacaa
     2341 cgccattgtt gtcagccttc ctcttcgaag gtactcccct ggacggaccc ggtggtcacg
     2401 cccgaaaccc caagaaccgc tcccccaacc ccctcccgtg gaaagagagg gcgcttttga
     2461 tccgtgcggc gtaaagcccc ctctccgcac aggggagggg gttgggggag cggttggtta
     2521 catcgccgcg gcgcggatgg cggccagcaa tgggccttcc atccggtgct tggcaataaa
     2581 agcggcagcg ccagctgccg ttgcgcgggc gatcgtctcg atgtcgtcgt gcaggctgag
     2641 catcacgatc gctgtctgtg gcagcgatgt ccgcaggttg cgggtggcat caatgccatc
     2701 catccctggc agctcgacgt ccatgacgac gacatccggc tggagccgcc cagccagttc
     2761 cagcgcgctc tcggcatcgc cggcctcgcc gacgatctcg acatccggct ccagtgcgag
     2821 ccgcatcatc aggccgcgcc ggacctgcgg ctgatcgtct accagcaata gcctgatcac
     2881 ccgattgaag tcccaacctg ttccaatcac cccgttgtgc cgcattgcct gctccattgc
     2941 agttatccgt tcgactgccg gccgagttct acgcctcgtt gcgccgcttg acggtcaccg
     3001 ttgaccgcta tcgcgtccgg tgtcataaga ataggttctt tgcgatctca caacaccgga
     3061 cgaacggccc gagcggctat ctggcctgtg gcggattggt catctccgtc aaaaggcgga
     3121 gacgatcgct cagggggtat cgcgctagtt cccaggaact gctagagtta tgtcgtcgaa
     3181 gcattgcaat gggaattgat gcgatgtgga tgccagccgg cgttcgcact cagtcgtcgt
     3241 tggttgtgtc acagtcaggg gagcaccagg gaaagcgatc cgcagatgga ttcagtttcg
     3301 atcaccatcg atgccgttga atacgagcgg cttcgcgaga gccagcgggt gctgctgacg
     3361 ctcttcgaaa atctgcccgt catgatttac cgctgcaaca acgacgccga ctggtcgatg
     3421 gagtttgtct cggagggctg ccgcggcctg accggctacg atccagccgc gttgacctcc
     3481 ggccgggtca cgtatgcctc gattatccag cctgccgatc gcgatcgggt ctgggatggc
     3541 gttcaagctg cccttcaggc acgctcgtcc ttcagcctga cctactgcat cacgactgct
     3601 tctggcgagg agcgctgggt ctgggagcag ggccagggcg tcttcgacag ccacggagag
     3661 ttgcaaggca tcgaagggtt catcatcgac gtc
//
LOCUS       DASUGJ010000624         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1631868, whole genome shotgun sequence.
ACCESSION   DASUGJ010000624 DASUGJ010000000
VERSION     DASUGJ010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631868"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..876
                     /locus_tag="VFV93_14245"
     CDS             187..876
                     /locus_tag="VFV93_14245"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5166560.1"
                     /translation="MSADDRPVGSTDDLPIVHELNTLEQLRAVSDPLRVAIFRLLRER
                     EHTVKELCDELGESSTRLYYHVGELERVGLVRLVRTESKSGTTHKYYRSVSQFLNVPT
                     SLLHDDSRSPEQEAAVDWFCSMLENSVMDVRQAFGSAGPDFDRDLTFVTRNYIRTTPE
                     RAREFVRKLDELQNELLAADDESATFRITFTASLVPTPRFLATPGRRPSRSGRPGRRS
                     EPAGVVQPTSE"
     gene            1043..>2278
                     /locus_tag="VFV93_14250"
     CDS             1043..>2278
                     /locus_tag="VFV93_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5166561.1"
                     /translation="MGDESTSIRDRFGALATMRLNRRSFVRLSAMAAGAVVSIPLLAA
                     CGGDDDDDDTPAATTTTGTSAEPTSTTAATEEASATTGSEASPTSAGASPTSGAGSPT
                     SGTGTGSGVIDGGDRNMGKTFEEPDSPGGTMIEASILDLRSLNPLLYNDNPSFSVGYM
                     IYQTLAEANPETLEPVGNLASGWEVNDDATVWTMYLREGVTWQDGEAFDAEDVKFTYD
                     MFANPDTGSNQTSTLNAKLDSVEIVDPLTVQFNLKIPVADFLLDIGGVYFLIIPEHIW
                     RDVAAADIQQHPGSTGADPAMVVGTGPFIFKEWITGDHVTLNKNPDYWDGDVYLDEFI
                     YKIVPDTSAAIAQLKTGEVDMFLQVPGAEAAGFDGTDVTITAFPTLSFNFYGTQLDET
                     KSTKFQDAKVRKALLYGIDR"
BASE COUNT          428 a          725 c          742 g          383 t
ORIGIN      
        1 tacttgatgc gttccgggcg tgggcgaggc tgcggccatc gcctctacgc gggatgatat
       61 gcgcttccgc gcacctgttg acgcaatttc ggccatcgtc tattctctct gacaccgttc
      121 aaatgacttt gaatcgacaa caccggctga gcggatcgtg tcgcgccgtg tcgggagggt
      181 acgtcaatga gtgctgatga caggccggtc ggatcgaccg atgatctgcc gatcgtccac
      241 gagctcaaca cgcttgagca gttgcgggcg gtctccgatc cgctacgggt cgcgatcttt
      301 cggctcctgc gcgaacgtga gcacaccgtc aaggaattgt gtgacgaact cggcgagtca
      361 tccacgcggc tctactacca tgtgggtgag ttggaacggg ttgggctggt ccggctggtg
      421 cggaccgagt cgaagtcggg gacgacccac aaatactacc ggtcggtgtc acagtttctg
      481 aatgtgccga cctcattgtt gcacgacgac agccgtagcc cggagcagga agcggccgtc
      541 gactggttct gcagcatgct ggagaacagc gtcatggatg ttcggcaggc gttcgggtcg
      601 gccgggccgg acttcgatcg cgatctcact ttcgtgacgc gcaactacat ccggacgacg
      661 cccgagcggg cgcgcgagtt cgtgcgcaag ctcgatgagc tgcagaacga gctgctggcg
      721 gccgacgatg agagcgcgac cttccggatc accttcaccg cctcgctcgt gccgacgccc
      781 cgctttctgg cgacaccggg gcgacggcca tcgcggtccg gccggcctgg tcgccggagc
      841 gaaccggcgg gagtcgtcca gccgacaagc gaatgagtcc gaaccggccg agcgatgcgt
      901 aggctggaga aacatgttag cgtacggcgc cggagtgcag ggtttccggc cgttgtacgg
      961 agtgaaaggg caggggccgg ccagcaggac cgacaggtgg gtgtccgagt accgtcagtt
     1021 gcactgacct acggagggtt gaatgggaga cgaatcgacc tcgattcgcg accgcttcgg
     1081 cgcgctcgcg acaatgcggc tgaaccgacg ttcgttcgtg cgcctctcgg cgatggcagc
     1141 cggagccgtt gtctccatcc cgctgctggc tgcctgcggc ggcgacgatg acgacgacga
     1201 cacgccggcc gcgaccacca cgaccggcac cagcgccgaa ccgaccagca cgacagccgc
     1261 gacggaagag gcgtccgcaa cgaccggcag cgaggcgagc ccgaccagtg ccggggcctc
     1321 gccgaccagc ggcgcaggat cgccgacatc cggcaccggc accggaagcg gggtgatcga
     1381 cggcggcgac cgcaatatgg gcaagacgtt cgaagagccg gattctccgg gcgggacgat
     1441 gatcgaggcc agcatcctcg acctgcgctc gttgaacccg ctgctctaca acgacaaccc
     1501 gtcgttctcg gtcggctaca tgatctacca gacgctggct gaggcaaacc ccgaaacgct
     1561 ggagccggtc ggcaatctcg cttccggctg ggaggtgaac gacgacgcca ccgtctggac
     1621 gatgtacctc cgcgagggcg tcacctggca ggatggcgag gcgttcgacg ccgaggatgt
     1681 caagttcacc tatgacatgt tcgccaaccc ggacaccggc tcgaaccaga cctcgacgct
     1741 gaatgcgaag ctggactcgg tcgagatcgt cgacccgctg acggtccagt tcaacctgaa
     1801 gatcccggtc gccgacttcc tgctggacat cggcggcgtc tacttcctga tcatccccga
     1861 gcacatctgg cgcgatgttg cggcggcgga tatccagcag caccctggct cgaccggcgc
     1921 cgacccggcg atggttgtcg ggaccggccc gttcattttc aaggagtgga tcaccggcga
     1981 ccacgtcacg ctcaacaaga atcccgacta ctgggatggc gacgtctacc tggacgagtt
     2041 catctacaag atcgtgccgg acacctcggc ggcgatcgcg cagctcaaga ccggcgaggt
     2101 cgacatgttc ctgcaggtgc cgggcgccga agcagccggc ttcgacggca ccgatgtgac
     2161 gattaccgcc ttcccgacgc tcagcttcaa cttctacggc acccagctcg acgagacgaa
     2221 gtcgaccaag ttccaggacg cgaaggtccg taaggcgctg ctctacggca tcgaccgg
//
LOCUS       DASUGJ010000625         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_380074, whole genome shotgun sequence.
ACCESSION   DASUGJ010000625 DASUGJ010000000
VERSION     DASUGJ010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380074"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFV93_14255"
     CDS             complement(<1..634)
                     /locus_tag="VFV93_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166562.1"
                     /translation="MTEHFICTTCGTQYAGSEQPPDGCTICLDDRQYVNPNGQQWTTL
                     EALRASGRQNVFTEIGPGLTTIQTDPSFGIGQRAHLIQTPAGNVLWDCVALLDDATIE
                     RVNALGGLAAIALSHPHFQTTSVEWSRAFNAPVHVHQADQQWTMRPDDAAIQYWPGEL
                     YEPILGITLIHCGGHFPGSAALHWADADGGQGALFTGDTIYVVADNRWVTF"
     gene            821..1174
                     /locus_tag="VFV93_14260"
     CDS             821..1174
                     /locus_tag="VFV93_14260"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX5166563.1"
                     /translation="MDDTQLRTYVLTKPGAEGGYPFGPGAFVAKVSGKMFALLSEGDD
                     PPTISLKVDPDEGELLRGQYPAIIPGYHLNKRHWITATIDGSLPPDLLPDLVDDSYDL
                     VVTSLTRAQRDALGQ"
     gene            1215..1571
                     /locus_tag="VFV93_14265"
     CDS             1215..1571
                     /locus_tag="VFV93_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166564.1"
                     /translation="MTEVVARLASFLKETLSVRGVASVLIVAGAIWVASGLRLRGRLL
                     AAWLLVPVLIAVASVCIAGVKRLDPDESLEGPHLIRVAHNDAITLSDLVGVAFAGMAC
                     LLAMYLIWRRIQARAG"
     gene            complement(1583..>2618)
                     /locus_tag="VFV93_14270"
     CDS             complement(1583..>2618)
                     /locus_tag="VFV93_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166565.1"
                     /translation="DAISLHPYVWNFTLEEYGRHLELNYDRINWLRDLMVERGDEAKP
                     VWITEFGWNNGVENQAKNLVYALEWLKQRPWIEFAHLHMLHDWNEEPLDAFGLMAITP
                     DRYGVPRLTPEAVFYPKQPFYDAFKNYPKDEVPARPWGNTGRYFPETGQTVSGRFLKA
                     WNERGGLRILGLPLTRPYPREQEDGSWLLVQDFERARLEFHPENVGSWSEVLGTLAGT
                     DITAGRHNEPAFRPLGSCTSTPGRNCYVETHHSLAYGFREFWEKHGGVATFGYPISEE
                     FDELNPDTGEVYTVQYFERARFEYHPEYAGTEYEVLLGLLIRDDLQRSGWLWPPEGSL
                     LPTARQYSSR"
BASE COUNT          428 a          834 c          866 g          490 t
ORIGIN      
        1 tgaacgtcac ccagcggttg tcggcgacga catagatcgt gtcgccggtg aagagcgcgc
       61 cctgaccacc gtctgcgtcg gcccagtgca gggcagcgct gccggggaag tggccgccgc
      121 agtggatgag cgtgatcccc aggatcggtt cgtacagttc acccggccag tactggatcg
      181 cggcatcatc cggccgcatc gtccactgct ggtcggcctg gtggacgtgg accggcgcgt
      241 tgaaggcgcg gctccactcg acggacgtcg tctggaagtg cgggtgggag agggcgatgg
      301 ccgccagccc gccgagcgcg ttgacccgct cgatcgtcgc atcgtccagc agcgcgacgc
      361 agtcccagag gacattcccg gctggtgtct ggatcaggtg tgcccgctgg ccgatcccga
      421 atgatggatc ggtctggatc gtcgtcaggc cggggccgat ctctgtgaag acgttctggc
      481 ggccgctggc gcgtagtgcc tccagcgtcg tccactgctg cccgttgggg ttgacatact
      541 gccggtcgtc cagacagatc gtgcagccgt ccggcggctg ctcgctcccg gcgtactgcg
      601 tgccacaggt cgtacagata aaatgctcgg tcatgagtca agacctttct gttgaacggt
      661 ggatgccagc catgaatgtc accaatcatc gccaatcgtc accaatcgta gcggcgagtc
      721 gatgactcgc ccaatacaca atgaccaatt ggcgcgttgg acgattgaca ttgaggcgag
      781 tcaccgactc gccgctacgt ttaccactat aggagccagc atggatgaca cacagctacg
      841 aacatacgtc ctcacgaaac ctggcgcgga aggcggctac ccgttcgggc ctggggcgtt
      901 cgtcgccaag gtcagcggca agatgttcgc gctcctctcc gaaggagacg acccgccgac
      961 gatctcgctc aaggtcgatc ccgacgaagg cgagctcctg cgcggtcaat atcccgccat
     1021 catccccggc tatcacctca acaagcgcca ctggatcacc gccacgatcg acggcagcct
     1081 cccacccgac ctgctgcccg acctggtcga cgactcgtac gacctggtcg tcacgtcgtt
     1141 gacccgcgcg caacgcgatg cgctcgggca ataggtcgtg tttgcgaagc gacacaggct
     1201 gagatgctga cgacatgact gaggttgtcg cgcggctggc gagtttcctc aaggagacct
     1261 tgtcggtccg aggtgtcgcg agtgtcctga tcgttgcggg cgcgatctgg gtcgccagcg
     1321 ggctgcggct gcgcgggcgg ctgctggccg catggctgct cgtgccggtc ctgatcgcgg
     1381 ttgccagcgt ctgcatcgcc ggtgtgaagc ggctcgatcc cgatgagtcg ctggaagggc
     1441 cgcacctgat ccgcgtcgcc cacaacgacg cgatcacgtt gtccgatctg gttggcgtcg
     1501 cgttcgccgg catggcctgc ctgctggcga tgtacctgat ctggcgccgc atccaggcac
     1561 gtgcagggta gctggcgcgg tgttaccggc tgctgtactg ccgggcggtc ggcaggagcg
     1621 agccttccgg cggccagagc cagccggagc gctggaggtc gtcgcggatc aaaaggccga
     1681 gcagaacctc gtactcggtc ccggcgtatt cggggtggta ctcgaagcgg gcgcgctcga
     1741 aatactggac cgtgtagacc tcgccggtgt cggggttcag ttcgtcgaac tcctcggaga
     1801 tcgggtagcc gaacgtcgca acgccgccgt gtttctccca gaactcacgg aacccgtacg
     1861 ccagcgagtg atgtgtctcg acataacagt tgcgaccggg agtcgaggtg cagcttccta
     1921 gcggcctgaa cgcgggttcg ttgtgcctgc cggcggtgat atcggtcccg gccagcgtcc
     1981 cgagcacctc gctccagctg ccgacgttct cgggatggaa ctccagccgc gcccgttcga
     2041 agtcctggac cagcagccag gagccgtcct cctgctcgcg cgggtagggg cgcgtcagcg
     2101 gcaggccgag gatgcgcagc ccgccccgct cgttccaggc tttcaggaat cggccggaga
     2161 cggtctggcc ggtctcgggg aagtagcggc cggtgttgcc ccacggtctg gccggcacct
     2221 cgtcctttgg gtagttcttg aacgcgtcgt agaacggctg cttggggtag aagacggcct
     2281 cgggcgtcag ccgtggcacg ccgtagcggt ccggcgtgat cgccatcagc ccgaaggcgt
     2341 ccagcggctc ctcgttccag tcgtgcagca tgtgcaggtg ggcaaactcg atccacggcc
     2401 gttgcttgag ccattccagc gcgtagacga ggttcttcgc ctgattctcg acgccgttgt
     2461 tccagccgaa ctcggtgatc cagaccggct tggcctcgtc gccccgctcg accatcagat
     2521 cgcgcagcca gttgatccgg tcgtagttca gctccagatg gcgtccgtat tcttcgaggg
     2581 tgaagttcca gacatacgga tgcagcgaga tggcgtcc
//
LOCUS       DASUGJ010000626         3822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1907970, whole genome shotgun sequence.
ACCESSION   DASUGJ010000626 DASUGJ010000000
VERSION     DASUGJ010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907970"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2145
                     /locus_tag="VFV93_14275"
     CDS             <1..2145
                     /locus_tag="VFV93_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166566.1"
                     /translation="GRSYWQLLSSSEGYSYYRPIPFLIWKALRDTQGHYDQATLHALP
                     LLAHAIAGWCLFVLLRRLGAGYWAAFPALLFLTFPFHYQNVAIVGTLFHPLTGAAMLA
                     SLVLYERARARDNDPQAASSTEEPPSEEEPNSPSVILSRSEGSLGVADMAGWIGGRAL
                     SSWEAIRRSRVRRSFAAAQDDRSTGAVLSTSFDWSLRVPNGTKWHLAALAATAVALWT
                     HESGVVVAVLIVLLEAVVLWQRGRRRPSTWVAAHLLAALLFVITWYSVEKAPFTDRTS
                     VDELQPKALFFLQGFTWPLSAQTEWIQDNLGIPGGITIASIGLLDVGVAALLGVFAAY
                     ALSAWRVRRWDLMAVPAVGLAIGVVASAPALWRLSWAYVENSPRLLYLVAIGASIFWG
                     LLPALRFENERLTAWWRVASSTLLLLVVAQSWRFVDVRMKMLASGTDIVNQIVATGET
                     YEGRQILVMNAPSWFSMTTYEYPYGHLGVQVMPEYIGLDRVIYTSSRRSAEVLAESGT
                     YNVDTSVGPYGFGPHGPEMTAEEIDALLRDGYELVDVKPDIDGYTVRDIGRIEPGGAD
                     PDLDHAGRIGEFVLVDDARVAADGDGMTVYIDWYVRTELARRIGTPASVTIVEVRDSS
                     GAPVYTYIGDALAGYSSPMLWRPNDLIADSIQIPLPEDGTYAVYAGIQQLFEPAPMPA
                     AGADGTAYDDGLLPIGEFVVTAGRLTSVESAP"
     gene            complement(2120..>3822)
                     /locus_tag="VFV93_14280"
     CDS             complement(2120..>3822)
                     /locus_tag="VFV93_14280"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX5166567.1"
                     /translation="WHAVRTGPATLYAGAVLLMAIAVVSLLGLITSGVSNDTGDWLLR
                     FALIVIVAILPLSFAISSIGSRLTGDRLMLVLATVLVLLSIVTIRSSVLAASERPVEP
                     GDPLSAGAVSESIPVLLTRIEKLSRDTTLSQRSSLDPTGGHGLRIAVDEAIAQPFAWY
                     FREYPNLTIFDPERESVPIDAEMVILAGGRDARIVAPGFSGQAYPFAATTPDVYRSPD
                     WSRLITGIVKPDEWRRFVDFLLNRNLDVEPVERQFQLLANATLAEKLFPATGPFTLSD
                     RPGAGAGQGQFNRPRGVQVAPDGSIYVVDSRNSRVEKFAPTGEFLLAFGSDGAGPGQL
                     ARFATSGGGGPNGIAIGDNGDIYVADTWNHRIQVYSSDGAYLRGWGSFFDAQDDPAQA
                     ATNGGQFYGPRGLAFYNGELYVTDTGNERVQVFTPEGGFVRMWGTTGSEQGNLLEPVG
                     IAVTADGTVLVADSHNARIARFDTNGDALDPWPVESWTGLTFFEPYVAVGPDGSVYVT
                     NSVNGTIDTFSADGTPGNPLGQGLVRQPFGIAVSLDGASLFVTDGALHAVVTVPIPPR
                     "
BASE COUNT          616 a         1214 c         1311 g          681 t
ORIGIN      
        1 ggccgttcct attggcagct gctgtcgtct tcagaagggt attcgtacta ccggccgatt
       61 ccgttcctga tctggaaggc actgcgcgat acccagggtc actacgatca ggcgacgctg
      121 cacgcgcttc cgttgctggc gcacgcgatc gccggttggt gtctgttcgt gctgctgcgc
      181 cggcttgggg cagggtactg ggccgcattc ccggcgctac tgttcctgac gttcccgttc
      241 cactaccaga acgtcgcgat cgtcgggacg ctcttccacc cactgaccgg cgccgcgatg
      301 ctggccagcc tcgtcctcta cgagcgcgcc cgtgcccgcg acaacgatcc gcaggcagcg
      361 tcctcaacag aagaaccgcc gtcagaggag gaaccgaact ctccctctgt catcctgagc
      421 cgcagcgaag gatcgctcgg cgtggcagac atggcggggt ggatcggtgg ccgagcgctc
      481 tcatcgtggg aggccatccg tcgctccagg gtcaggcgat ccttcgctgc ggctcaggat
      541 gacaggtcaa cgggtgctgt cctctctacg tcattcgact ggtctttgcg agtgccgaac
      601 ggcacgaagt ggcatctggc ggcgctggcg gcgacggccg ttgcgctctg gacgcatgaa
      661 tctggcgtcg ttgtcgcggt gctgatcgtg ctgcttgagg cggtcgtgct ctggcaacgc
      721 ggtcgccggc ggccgtcgac gtgggtcgca gcgcacctgc tggctgcgct attgttcgtc
      781 atcacctggt actcagttga gaaggcgccc ttcaccgacc ggacgtcagt cgatgagttg
      841 cagccgaagg cgctgttctt cctgcagggt ttcacctggc cactctctgc tcagactgag
      901 tggattcagg acaacctcgg tatccccggc gggatcacga tcgcgtcgat cgggctgctg
      961 gacgttggtg ttgccgcgct gctgggcgtg ttcgctgcgt acgcgctatc ggcctggcgg
     1021 gtgcgtcgct gggacctcat ggccgtgcct gccgtcggac tcgcgatcgg tgtcgttgcc
     1081 agtgctccag cgctctggcg gctgtcgtgg gcgtacgtcg agaactcgcc acggctgctt
     1141 tatctggtcg cgatcggggc gtcgatcttc tggggtctgc tacccgcgct ccgcttcgag
     1201 aacgaacggc tgacggcctg gtggcgggtc gcgtcctcga cgctgttgct gctggtcgtc
     1261 gcacagtcgt ggcgcttcgt cgatgtacgg atgaagatgc tggccagcgg aactgacatc
     1321 gtcaatcaga tcgtggcaac cggcgagacg tacgagggac gacagatcct tgtcatgaac
     1381 gcgccgtcgt ggttctcgat gacgacatac gagtacccgt atggtcatct cggcgtccag
     1441 gtcatgccgg agtacatcgg gctggatcgg gtcatctaca ccagttcgcg gcggtcggct
     1501 gaggttttgg cggaatccgg cacctacaac gtcgacacga gcgtcgggcc atatggcttc
     1561 ggcccccacg ggccggagat gacggccgag gagatcgacg cgttgctgcg cgacgggtac
     1621 gagctggtcg atgtcaagcc ggatatcgac ggctacacag tccgcgacat cggccgcatt
     1681 gagccgggag gggcagatcc ggatctcgac cacgccggcc gaatcggcga attcgtgctc
     1741 gtcgatgacg cccgtgtcgc agccgacggc gacgggatga cggtttacat cgattggtat
     1801 gtgcgtactg agcttgcccg gcgaatcggt acgccggcca gtgtgacgat cgtcgaggtc
     1861 agggattcga gcggcgcgcc cgtgtacacc tatatcggcg acgccctcgc cggctactcg
     1921 tcgccgatgc tgtggcgtcc gaacgacctc atcgccgact cgatccagat cccgctgccg
     1981 gaggacggga cgtacgctgt ctacgccggc atccagcaac tgttcgagcc ggcgccgatg
     2041 cctgcggccg gtgcagacgg taccgcctac gacgacggcc tgctgccgat cggtgagttc
     2101 gtcgtgaccg ccgggcggct tacctcggtg gaatcggcac cgtgacgacc gcgtgcagcg
     2161 cgccatcggt gacgaacagc gacgcgccgt ccagcgagac cgcgatcccg aacggctggc
     2221 ggaccagtcc ctgcccgagc ggattgccgg gagtgccgtc cgcgctaaac gtgtcgatcg
     2281 tgccgttgac cgagttggtc acgtagacac tgccgtccgg tccaaccgcg acatatggct
     2341 caaagaaagt tagcccggtc cacgactcga ccggccacgg atcgagcgcg tcgccgttgg
     2401 tgtcgaagcg ggcgatccgc gcgttatggg agtcggcgac aagaacagtg ccgtcggccg
     2461 tgacagcgat cccgaccggc tccagcaggt tgccctgctc gctgccggtc gtaccccaca
     2521 tccggacgaa gccgccttcc ggtgtgaaga cctggacgcg ctcgtttccg gtatcggtga
     2581 cgtagagctc gccgttgtag aaggcgagcc cgcgcgggcc gtagaactgg ccgccgttgg
     2641 tcgcggcctg ggccgggtcg tcctgggcgt cgaagaagct gccccagccg cgcagatagg
     2701 cgccatcaga cgagtagacc tgaatgcggt ggttccaggt gtcggccacg tagatgtcgc
     2761 cgttgtcgcc gatcgcgatc ccgttcggac cgcccccgcc ggaggtcgcg aagcgggcca
     2821 gttggccggg gccggcgccg tcgctgccga acgccagcag gaactcgccg gtcggcgcga
     2881 atttctcgac gcggctgttg cgcgagtcga cgacgtagat cgagccatcc ggcgcgacct
     2941 gcacgccacg cgggcggttg aactgcccct gtccggcgcc ggcgccgggg cggtcggaga
     3001 gggtgaacgg tccggtcgcc gggaagagtt tttcggccag cgttgcgttg gcgagtaact
     3061 ggaactggcg ttcaaccggc tcgacatcca aattgcggtt gagcaggaaa tcgacgaagc
     3121 gccgccactc gtcgggcttg acgatgccgg tgatcagcct gctccagtcg ggcgatcggt
     3181 agacgtccgg tgtcgtcgcc gcgaacggat atgcctgacc ggagaagccc ggcgcgacga
     3241 tgcgcgcgtc acggccacca gccaggatca ccatttcggc gtcgatcggg accgattcgc
     3301 gctccggatc gaagatcgtc aggttcgggt actcgcggaa gtaccaggcg aacggctgcg
     3361 cgattgcctc atcgacggcg atccgcaggc cgtgaccgcc ggtcgggtcg agcgagctgc
     3421 gctggctcag cgtcgtgtcg cgcgagagct tctcgatccg ggtgaggagg accgggatgc
     3481 tctcgctgac cgcaccggcg ctgagcggat cacccggctc gaccggccgt tcggaggcgg
     3541 ccagcaccga cgagcggatc gtcacgatcg agagcaggac cagcacagtc gccagcacca
     3601 gcatcagccg gtcgccggtc aggcgcgagc cgattgacga aatcgcaaac gagagcggca
     3661 ggatcgcgac gatcacgatc aacgcgaagc gcagcagcca gtcgccggtg tcatttgaga
     3721 cgccggaggt gatcaggcca agcaggctga cgacggcgat cgccatcagc aggacggcgc
     3781 cggcatacaa cgtggccggg ccggtccgaa ccgcgtgcca cg
//
LOCUS       DASUGJ010000627         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_520102, whole genome shotgun sequence.
ACCESSION   DASUGJ010000627 DASUGJ010000000
VERSION     DASUGJ010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520102"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFV93_14285"
     CDS             <1..290
                     /locus_tag="VFV93_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166568.1"
                     /translation="LGLAFLQGNGVPSDLNGDLIVAQHGSWNRQPPAAPKLLLIEFQN
                     GSPIGARDFVTGWQDAAGERWGRPAGVVVAPDGSLIVSDDTAGLLYRISAG"
     gene            332..817
                     /locus_tag="VFV93_14290"
     CDS             332..817
                     /locus_tag="VFV93_14290"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEX5166569.1"
                     /translation="MSTPDQTDAARCAPGMYRIDDAARQRVRAGDVFPPGWAVAILDG
                     AEAGTRAGFFTAIARELHFPDYFGHNWDAVYDCLTDFNWLPAAGYGIVLDGYGALATA
                     QPEQWQVALKVLGEASAFWQATKRPMVVLLYGSEADAPDVPILPADCLPVIHARASGG
                     A"
     gene            1080..2198
                     /locus_tag="VFV93_14295"
     CDS             1080..2198
                     /locus_tag="VFV93_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166570.1"
                     /translation="MVGRVRLSLRSLALLISIAIGMLLPADRAVAQPTGLKDMIYVSP
                     HWGYSIRWYSSEWIIGDEQSDADADRLQLTDSLGNAIIYTGLEGFQGDADACLDQMVR
                     DVVSIPGAGDVAQAEGDFGTPYDWRDASQSYTVFQIRLPVNGVLVDHAVYAECQTLVP
                     GKAVFQRLYTGPVEVFDQYYDDIAQTLDGVFLPASAWLPMPDSEVVWAGSAPLLSTSR
                     LNASLVPSASHAQLLVGLVDASGDTRIVSFENVSDAPVSVDPNSLVLSVTAMTDETLP
                     PEQRPSSIAWEDRSVASADGTRTLAPGERATVQVEIPPVDTSTMTCDGLPSLTLEYRP
                     ADGESVTIGSSALALRECLNNVVAAPAGGSRPRLRLAR"
BASE COUNT          335 a          713 c          771 g          407 t
ORIGIN      
        1 cgctgggtct ggcgttcttg cagggcaatg gcgttcccag tgacctgaac ggggatctca
       61 tcgtcgccca gcatggctcc tggaaccggc agccgccggc cgcgccgaag ctgctcctga
      121 tcgagttcca gaatgggtcc ccgatcggcg cgcgggactt cgtcaccggc tggcaggatg
      181 ccgccgggga gcggtggggc agaccggcgg gcgtggtcgt ggcgccggac ggcagcctca
      241 tcgtttccga cgacacagcc ggcctgctct atcggatcag cgcaggctga tccccccgcg
      301 gccgctatgc ttggtgaggg agggaccggc cttgagcaca cccgatcaga ctgacgccgc
      361 tcgctgcgcg cctggcatgt accgcatcga cgatgcggcc cggcagcgag tgcgcgccgg
      421 ggacgtcttc ccgccgggat gggctgtcgc catcctcgat ggcgctgagg ctggcacgcg
      481 ggccgggttc tttaccgcga ttgcccgtga gctgcacttt ccggattact tcgggcacaa
      541 ctgggatgcg gtctatgatt gtctgaccga tttcaactgg ctccccgccg ccggctacgg
      601 catcgtcctc gacggctatg gtgcgctggc tacggcgcag ccggagcagt ggcaggtcgc
      661 cttgaaggtg ctgggcgagg cgagcgcgtt ctggcaggcg acgaaaaggc cgatggtcgt
      721 cctgctctac ggttccgagg cagacgcgcc ggatgtgccg attttgccgg ctgactgtct
      781 accggtgatt cacgccagag cgagtggcgg cgcctgaacc ggccataacg tctctctggt
      841 ctcaggatcg acgacccacc tccgcgggcc cgtcggttgc gctacactgc gggccatccg
      901 ttcgattgga ttcttgctga tctcctcgag cgcagtctgc cgcaccgcga tctggtgagg
      961 cggcattggc tgatccagcg tgatgcgggc gacctgtcac cacggacctc gccgggagtg
     1021 cggcaggatg gcgtagcgac gctgaccgtc gttcgcgcgg tgtttgcgag ggggtcccga
     1081 tggtcgggcg cgtccggctc tctcttcgca gcctggcgct gctcatctcg atcgcgatcg
     1141 ggatgttgtt gccggcggat cgtgccgtgg cccagccgac cgggctgaag gacatgatct
     1201 acgtcagccc gcattggggt tactcgattc gctggtactc cagcgagtgg atcattggcg
     1261 acgagcagtc ggacgccgac gcggatcggc tgcagctcac cgacagcctc ggcaatgcca
     1321 tcatctacac gggtctcgag ggcttccagg gagacgccga cgcctgcctc gatcagatgg
     1381 tgagggatgt cgtgagcatc cccggcgccg gcgacgtcgc acaagccgag ggtgatttcg
     1441 gcacaccgta cgactggcgt gatgcctcgc agtcctacac cgtcttccag atccggctgc
     1501 cggtgaatgg ggtgctggtc gaccacgcgg tctacgcgga gtgtcagacc ctggtgcctg
     1561 gcaaggcggt tttccagcgg ctctacacgg ggccagtgga ggttttcgac cagtattatg
     1621 acgacatcgc gcagacgcta gatggcgtct tccttcccgc cagcgcatgg ctaccgatgc
     1681 cagattcgga agtggtctgg gcaggaagcg ctccgctgct gagcacatcg cggttgaacg
     1741 cgtcgctcgt cccgagcgcg tcgcacgccc aactgctggt gggtctcgtc gatgcatcgg
     1801 gcgacacgcg catcgtctcg ttcgagaacg tcagtgatgc gccggtcagc gtcgacccca
     1861 acagccttgt cctctccgtg acggcgatga cggacgagac cctgccgccg gagcagagac
     1921 catcttccat tgcctgggaa gacagatccg tcgcgagcgc ggacgggaca cggaccctgg
     1981 cgccgggcga gcgggcgacg gtccaggtcg agatcccgcc ggtagacacg tcgacgatga
     2041 cctgcgacgg cttgccgtca ctcaccctcg agtaccggcc ggcggacggc gaatccgtga
     2101 cgatcggctc gagtgcgctt gctcttcgcg agtgcctcaa caatgtcgtc gccgcaccgg
     2161 cgggcggcag ccggccccgg ttgcggctcg ctcgctgatc gaggggcgtg gaggggaatg
     2221 gtggcg
//
LOCUS       DASUGJ010000628        17780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1632840, whole genome shotgun sequence.
ACCESSION   DASUGJ010000628 DASUGJ010000000
VERSION     DASUGJ010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 17780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17780
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632840"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFV93_14300"
     CDS             complement(<1..494)
                     /locus_tag="VFV93_14300"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5166571.1"
                     /translation="MALAGDIEDYPLAELLFFLSSKQRTGELVLEQAEITIVFALRHG
                     RLIAAQMIPTDQRLGERLVSGGVITPSMLSDALQRQEQTPERQLGAILVERGDAPRET
                     VIDAIRGQITDCLVAFLIAPGGTFLFQEKPLDTDMIDVDVIVEREVLDALRRADEYVT
                     RQIDT"
     gene            complement(513..944)
                     /locus_tag="VFV93_14305"
     CDS             complement(513..944)
                     /locus_tag="VFV93_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5166572.1"
                     /translation="MCATTATRLASGVELAMPRLLGDDGTIVACLRRFDGSQMTIGSS
                     QSAGLCLWGDPFVEPEHACIRWVESAQAHVLEDWGSGERIELDGVRLKRPTRLTNGAR
                     FRVGTTDLVYCCRLPMPGYTSTLAQRIRPSQRYRIQVGSSS"
     gene            1455..3077
                     /locus_tag="VFV93_14310"
     CDS             1455..3077
                     /locus_tag="VFV93_14310"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX5166573.1"
                     /translation="MAYDVLIRGGKVIDGSGNPWFYGDVALEGDKVAAVAPRGRIDPA
                     NAAEVVDATGHVVCPGFIDIQSHSIMPLFADGRLLSKAHQGVTTEIMGEGWTPAPFGG
                     RIKEPMRSGIIAGGVSEQWAEKAKEWTRFRAWLEAVEANGSSLNIGSFVGGGTIREYA
                     RGWDMGEPTAEEVATMCRVMDECMQEGAFGIATALIYPPGSYAGTDELVEIAKVVGRY
                     GGVYITHIRSESDLILDGMGEAIEIGRRGQCSIEIYHLKASGQSSWQLMPKAMEMIDE
                     ARASGVDITADMYPYIASGTGITVLIPAWAHEGGKLYENLADEKTRAIIKAEMMDPPL
                     ESVGASRASNTDGVMPVGFFKPENRQYIGKNLTEIAEMRGQHWSDAVMDLLLSEQQRI
                     STMYFMMSEENVKRQLQAPWNKVSTDAGGFDPATQDTPTHPRAYGTYPRVLGKYVREE
                     GVLQLEDAIRKMSSAVADRLSLRDRGQLRTGMMADVVIFDPETVIDHSTFTEPHQLST
                     GIRDVWVNGSRVLQNGTHTGAMPGRIVDGPGR"
     gene            complement(3258..3584)
                     /locus_tag="VFV93_14315"
     CDS             complement(3258..3584)
                     /locus_tag="VFV93_14315"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5166574.1"
                     /translation="MATTQQMPEIMTSEQAAEYLQVNRETIYCYIRQGRLRASRLGRT
                     YRIPRQGIEHLLWATRTRPDIHLREYTREEIDAFLQRDQMDDEARQIARWFDFDPAVK
                     LPIRDR"
     gene            3950..5716
                     /locus_tag="VFV93_14320"
     CDS             3950..5716
                     /locus_tag="VFV93_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5166575.1"
                     /translation="MGRTSRELADENFARWQWFYKKGMIDRRTFVKATMAWSGAAAMG
                     GSLLAACGGDDDDDDSGSGGAATTASGGATTVSGTGAQPTATTAAGAAPTSGGGGGTT
                     EGGTLRVALQTSENQGMDPHMHNQRTGIIFFYHTHDNLGVRDMSTMQIGPWLAESWEV
                     TDPTTWNMKLRQDVKFHNGDPFTAETVKWNWERVINPEQKSQQAGNHAQIAGVDIVDE
                     YTVNVRTKVPYPIFTERLQNFQMIPEKLAQEKGDAWLAENPVGSGPYKLVEWKKGSEY
                     VLTRNDDYWNKDVTIAYKDLILRTMPELSTQLAELLAGGLDIIRVVPFDQMKVVTDSG
                     VATPKVQDILRVQFVVLDAKGRGTPDNPFTDVRVRNAANHAIDVQTYIDTLQAGGDRT
                     PALVNPKHFGFDPSIVPHEYDPEKAKALLKEAGYEDGFDVKWKTGPQLMPNATQVEQA
                     MQRDLAAVGINAEFEIVADGSVSSQQTNEGKNPPMASTSWGSYSVFDADGILWDMLHS
                     TSIFSYYENKEFDALIDEARGILDSDKRKELYSSAQKILREEAPMLFMWGLHTVWGVN
                     NDVDWTPAPDEIDRYFWAKPKA"
     gene            5820..6749
                     /locus_tag="VFV93_14325"
     CDS             5820..6749
                     /locus_tag="VFV93_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166576.1"
                     /translation="MVSYIINRLIQMVVVIFGISVIVFVITNMLGDPVGVLLPPETPY
                     EQREIFREEMGFNRPLIVQYGDFLTDAVRGDFGDSFRSGRPASTLVLERMPATLTLTF
                     TAILLATLISIPMGIISAYKRHSVWENFATLFVVTGQAMPIFWLGLLLIIIFGVNLKW
                     LPVSGYGDGGIDRLKHLIMPAFVLGFFIAPVTERLVRSGMLEALDQDYVRTARAKGLA
                     ERVVLFRHAFRNTMIPVITVLGLQFGQLLGGAVITEAVFAWPGVGQLAIESIRGADFP
                     VTQAAVVLLAVIICLVNLGVDLAVGGLDPRIRR"
     gene            6801..7736
                     /locus_tag="VFV93_14330"
     CDS             6801..7736
                     /locus_tag="VFV93_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166577.1"
                     /translation="MATAEIQPQESIAQAFAHNRVRRRTMKSMMAAAWKLRMSVAGGI
                     VLLILALMAIFAPLLTPYTTEEGSVRERLQPPVWQEGGTWDHPLGTDGIGRDYATRLL
                     YGGRIALSVGLLATLISAAIGITLGMAAGYFGGWLDGIISSLVNIMITFPFILLALAV
                     MAVLGTSFRNVVIVLGIGAWPIYTRVIKFEVERIKELEYMAASRVIGISIWRSMWRHI
                     APNLINTVIVIGTVQVARLIISEAFLSYLGLGVPPPTPSWGFMLNESIPFSFSWSDIW
                     LPTLPGLAIFITTLSINFVGDGLRDLMDPRERASL"
     gene            8096..9862
                     /locus_tag="VFV93_14335"
     CDS             8096..9862
                     /locus_tag="VFV93_14335"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5166578.1"
                     /translation="MSSWNPSEMNDKIYANWERFYKEGRIDRRTLVKAAMVWAAGAGA
                     MGALAACGSDDDDDDDGGAASTSASGSGGQAETPAAGEPTKAAGGAEPTSAGSGSGDT
                     ADGGTLRVILDQSDLPTMDPHMHNLRTGIIFFYHTHDNLGLRNKETNQVEPWLAESWE
                     NIEPTVWEMKLRQDVKFHNGDPFTAETVKWNWDRVTNPEQKSQQIGNHAAIAGVEVID
                     DFTVHVTTKEPYPIFAERLQNFQMIPEKLAQEKGDAWLAENPIGSGPYKFVEWKKGQE
                     IVVTRNDEYWNPDVKPAYKDLIFRIVPGVPTQLAELLAGTADIIRVVPFDQMKAVDDS
                     DVAVTKTQAILRVGFTRLDAMGRTAPNPFQDVRVRHAANHACDIQGYIDALQPGGDRT
                     PALLNPMHFGFDPSIEPHEYDPDKAKALLEEAGYPDGIDVTWVRGPSSMPNQDQVDQA
                     MQRDLEAVGIRCTFETLSDGNVFTTRHNEGQAGPMHSYNWGSYSVFDADGIYWDQLHS
                     SSIFTYYNNPDLDKLLEDGRGSLDPEERKRIYSDAQRIVRDDAPMIFMWGFHSVWGVS
                     SKVEWSPRADEIDMYFTASPKA"
     gene            9963..10892
                     /locus_tag="VFV93_14340"
     CDS             9963..10892
                     /locus_tag="VFV93_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166579.1"
                     /translation="MGSFIAHRVLQTIIAVFGISVIVFVITNMIGDPVNVLLPPETPQ
                     EQREIYREEMGFNRPLIVQYWDFVSDAVQGDFGVSFRAERPAMTIVWERMPATLLLTL
                     SGTIVATLIAIPMGILSSYKRYSIWDNISTLFVVAGQAMPIFWLGLMLIIVFGVNLKW
                     LPPSGYGEFGIDRLKHMVLPTIVLGVFLAPVTERLVRSGMLDALTQDYVRTARAKGLR
                     ERVVLTRHALRNTMIPVITVLGLQFGQLLGGAVITETVFAWPGVGLLAIDSIRNADFP
                     VTQAAVVSLAIIIGLVNLGVDLVVGMLDPRIRR"
     gene            10957..11886
                     /locus_tag="VFV93_14345"
     CDS             10957..11886
                     /locus_tag="VFV93_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166580.1"
                     /translation="MATADPTIQDAASAFSPQRTRKKTFRQWMQTFWKLRMSVAGATI
                     LAILIFIAIFAPLLTPYSYTEGRITERLQEPVWQGGTWSHPLGTDGIGRDYATRLMYG
                     ARIALSVGLLATLISGAIGITLGLLAGFFGRWVDGIISTLVNVMMTFPFILLALAVIA
                     VLGASFTNVVLVLGVGAWPIYTRVVTFEVERIKSLEYIAAGRVVGLGNMRLMVRHIMP
                     NLFNTIIVIATVQVARLIISEAFLSFLGLGVPPPTPSWGYMLQESQAFMFTWGDLWLP
                     TLPGLLIFITALSINFVGDGLRDLMDPHQRASL"
     gene            12118..13245
                     /locus_tag="VFV93_14350"
     CDS             12118..13245
                     /locus_tag="VFV93_14350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5166581.1"
                     /translation="MQVTDLHGTPFADTYGQVLRDYLQTRREDALYRASLLSQTFVER
                     GVGPEEIIALHFEQLDQALDGLSYRVQARSIGDAHQFLLEVMITYGVHYKEYLELRMR
                     EGVQAAEAEAELERRLRQERERIDQERTELLEHIAHELRTPITTALGTLDLARRSIAR
                     GNFHRLEPLIGTAREAVDRLSRLSAQLVQISHGKLPDLERKPQDISALLTQACAWAEA
                     SAVEKQLQFQCGPFNEHVYVIGDADALLSLFGNLLSNAIRYTPVGGTVSMTCHVEGET
                     VFIECRDTGIGMSHDVQARIFERFFRAPEARNVDAQGLGVGLSLVHALVQAHDGAISV
                     SSVTGEGSVFGVTLPVMREGDGHGAELAFDEMSESTEETTN"
     gene            13242..13718
                     /locus_tag="VFV93_14355"
     CDS             13242..13718
                     /locus_tag="VFV93_14355"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5166582.1"
                     /translation="MSENVVRQQQSKLEQQRRTIAELQTPVIQVWKGILALPIVGSLD
                     TVRAQEMIEALLDRIVETNSDVVILDITGVPVVDTAVAKHLLETVAAARLLGTEVLIV
                     GLSTRTALTLVQLGIDLSHVTTRTTLAKGLELAFDRLGLEVVSKRRESPAREADEV"
     gene            13715..14101
                     /locus_tag="VFV93_14360"
     CDS             13715..14101
                     /locus_tag="VFV93_14360"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5166583.1"
                     /translation="MTRVPIVPIDDILVATVLEDLSDQDAIRLQHDLCLQIERNGAVG
                     VLLDLSVVEIVDSFLGRLISEVVASSRLLGAETVVVGIQPAVAITLVELGLDLRGVRT
                     ALTPKKGRRLLNQLIAASRSGIRHGH"
     gene            14091..14501
                     /locus_tag="VFV93_14365"
     CDS             14091..14501
                     /locus_tag="VFV93_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5166584.1"
                     /translation="MATEERIDLVGEESIVVARRRAREYVRGLGFGLLDQSRIATAVS
                     ELTRNVIRYAIDSRGEVVIRELPESGTRIGVEIVVSDAGPGIDDVSQAMRDGYTSGRG
                     LGLGLPGTRRLVDEMEIDSGRGRGTTVTIRKWRP"
     gene            14434..14913
                     /locus_tag="VFV93_14370"
     CDS             14434..14913
                     /locus_tag="VFV93_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5166585.1"
                     /translation="MRWRSIPDAGVGRRSRSANGVLDVESVITIDIRTRPDVDLARRE
                     VRRLAASAGLNFHDVEMVALAVTELAFNLVRYAQGGSIVLSRAISEGRTGVQIESRDT
                     GPGIDDLVLAMQDNYSTGGGLGSGLPAVKRLMDEFTISSTPKGTRVIARKWMTPNRR"
     gene            14889..15497
                     /locus_tag="VFV93_14375"
     CDS             14889..15497
                     /locus_tag="VFV93_14375"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5166586.1"
                     /translation="MDDSQSPLIIAIASRPHPHFSINGDQSSVHWRDGACVVTVVDGL
                     GHGPEAADAASLAIESLAVGADLSPGEAITRCHDRLFRSRGAAMAVARVDLGAGQLTF
                     AGVGNVEAQFWNGTRVQRPIAQRGIVGATLPSIREQVIELNDAGWLLLMFSDGIHQRF
                     DLELLPEFVARDAQTLADRILSDWSRETDDATVLVLMPRVTQ"
     gene            complement(15516..16262)
                     /locus_tag="VFV93_14380"
     CDS             complement(15516..16262)
                     /locus_tag="VFV93_14380"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5166587.1"
                     /translation="MSIPHETYQDDLPSYALGTLEPPATTRLERHLGECADCRRLLVE
                     YEEVMRLLPLGLPRSEPSPAARRELFNRVRTDTAATRRGADGWWPRFRLPALATVAII
                     VAIVVGALFSSLGNHDEANNAAAVVAELQESPDTQIIPMLGSDAAPDAVAQLFFQPGE
                     TTAGLVVSGLPPLPDDRAYQLWFVQPDETRHDGGVFDVDSSGQAIVVIEAPADYAPGW
                     RCGVTEEPAGGSEAPTGQNVMRGDYTDHEW"
     gene            complement(16259..16912)
                     /locus_tag="VFV93_14385"
     CDS             complement(16259..16912)
                     /locus_tag="VFV93_14385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5166588.1"
                     /translation="MPAFFVTVPDDRAGQHSALNAARVSALADAELMERVRRREEAAL
                     AALYDRYGGLILTVALRVVGDRDLAEEVMQDCFLRVWNASETYRAGRGQVSGWLIGIA
                     RNRAIDVLRSRQHKARMRERTPLPEPGEEDSFGVPDETEAVATRQAVADALSGLTMAQ
                     RQVVELAYYGGMSQSEIARQLGEPLGTIKSRTRVAMEHLRTALRPHFKPDDESEIRQ"
     gene            17042..17719
                     /locus_tag="VFV93_14390"
     CDS             17042..17719
                     /locus_tag="VFV93_14390"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5166589.1"
                     /translation="MSVQPQLLVVDFAGTMFREEGAVVVAYRQAFAAFGIDATDDDIA
                     ARRGASKRAVFEELAARNRPRAEAPAIAGHALAAFEEALRAEYSTGPVQEVPGAGEAA
                     GRLRAAGIKVAIASGFDRPLVDLLIARLGWHDLFDLTIAGNDVAAGRPSPAIIYHAMT
                     RLGVNDVGAVAAAGDTPLDLQAGTNARAGWVIGVLSGAHGIDTLGRTRHTHLLASVAE
                     LPELFSV"
BASE COUNT         3213 a         5662 c         5630 g         3275 t
ORIGIN      
        1 gtgtcgatct gccgcgtcac gtattcgtca gcgcgccgga gcgcgtcgag cacctcgcgt
       61 tcgacgatca catcgacgtc gatcatgtcg gtgtcgagcg gcttctcctg aaacaggaac
      121 gtgccgcctg gagcgatcag aaacgccacc aggcagtcgg tgatctgccc acggatggca
      181 tcgatcaccg tctcgcgcgg tgcgtcgccg cgctcgacca ggatcgcgcc gagttggcgt
      241 tcgggcgtct gctcctgcct ctggagcgcg tcactcagca tcgatggcgt gatgacgccg
      301 ccggagacca gccgttcgcc gagccgttgg tcggtcggga tcatctgggc cgcgatcagc
      361 cgcccgtgac gcagcgcgaa gacgatcgtg atctccgctt gctccaggac gagttcgccg
      421 gttcgctgct tgctgctcag gaagaacagc agttcggcaa gtggatagtc ttcaatgtct
      481 cccgcaaggg ccactgtcgt ctcctcccag ggtcaggaac tggacccgac ctgaatccgg
      541 tatcgttgag atggacgaat acgctgggcc agtgtcgagg tgtagcccgg catcggcagc
      601 cggcagcaat agaccaggtc ggtcgttccg acgcgaaagc gagcgccatt ggtgaggcgg
      661 gtgggacgct tcaggcgaac cccgtcgagc tcgatccgct ctccgctgcc ccagtcttcg
      721 agcacgtgtg cctgcgcgga ttcgacccag cggatacagg catgttccgg ctcaacgaac
      781 ggatcgcccc acagacacag gccggctgac tgactactgc cgatggtcat ctggctgccg
      841 tcgaaccgcc tgaggcaggc gacgatcgtg ccatcatcac cgagcaaacg cggcatcgcc
      901 agttcgactc ctgaagccaa cctcgtcgcg gttgtcgcgc acacgccagg acctccttga
      961 gcaacatgta acgatgatgt cgtggcccac tggccgtccg ccctggaacc catcgcttcg
     1021 tcgcgctgcg ttcatgtacc acgcccggac gctccgcccg cgatgcgaca cgacgcacgg
     1081 ccgctacgat catgagtacc aatccagccg acacctatag cccatttgtc ttgggaaaag
     1141 ccggtactta tcgggtttga ggaactatca gcatcgaagt tgccagatgc gcagtgtcaa
     1201 gatgaatttc tcgtcagcaa gcggcatcga atttgtcatg aagctgacaa cgatgtgcgg
     1261 tagttcagtc acactcagcc gaaaacagaa cccactggtc catccgtgac acgcgccgat
     1321 tcggcctgcg cttccctgct ggatcgcgtc acgcgtcacg cgtcacacgt taccgacggg
     1381 cttgcgcgtt cctcgacgcc ggtcataatc agccgagtga gtggctcggc tgatccgggg
     1441 atgagggagg aaccatggcg tacgatgttc tgattcgcgg cggcaaggtg atcgacggca
     1501 gcggcaaccc gtggttctac ggcgacgtcg cgctggaagg tgacaaggtc gccgccgtcg
     1561 cgccacgcgg ccggatcgac ccggccaacg ccgccgaggt cgtcgacgca accggccatg
     1621 tcgtctgtcc cggcttcatt gatatccagt cgcactcgat catgccgttg tttgccgacg
     1681 gccgcctgct ctcgaaggcg caccaggggg tgacgaccga gatcatgggc gaaggctgga
     1741 ccccggcgcc gttcggtggc cggatcaagg agccgatgcg ctccggcatc atcgccggtg
     1801 gcgtctcgga gcagtgggcc gagaaggcca aggagtggac ccgtttccgc gcctggctgg
     1861 aggcggtcga ggccaacggc tcgtcgctca acatcggctc gttcgtcggc ggcgggacga
     1921 tccgcgagta tgcccgtggc tgggacatgg gtgagccgac cgccgaagag gtcgcgacga
     1981 tgtgccgcgt gatggacgag tgcatgcagg agggcgcgtt cggcatcgcg acggcactca
     2041 tctacccgcc cggctcctat gccggcaccg acgagctggt cgagatcgcc aaggtcgtcg
     2101 gccgctacgg tggcgtctac atcacccaca tccgctccga gtccgacctg atcctcgacg
     2161 ggatgggcga agcgatcgag atcgggcggc gcgggcaatg ctcgatcgag atctaccacc
     2221 tgaaggcgtc cgggcagtcg agctggcaac tgatgccgaa ggcgatggag atgattgacg
     2281 aggcgcgcgc ctccggcgtc gacatcacgg ccgacatgta cccctacatc gccagcggga
     2341 ccggcatcac cgtcctgatc ccggcctggg cgcacgaggg cggcaagctc tatgagaacc
     2401 tggccgacga gaagacgcgt gccatcatca aggccgagat gatggacccg ccgctggaat
     2461 cggtcggcgc ctcgcgggcc agcaacaccg acggcgtcat gccggtcggc ttcttcaagc
     2521 ccgagaaccg gcagtacatc ggcaagaacc tgaccgagat cgccgagatg cgtggccagc
     2581 actggtccga cgccgtcatg gacctgctgc tgtccgagca gcagcgcatc agcacgatgt
     2641 acttcatgat gagcgaggag aacgtcaagc gccagctcca ggcgccatgg aacaaggtca
     2701 gcaccgacgc cggcggtttc gaccctgcta cccaggacac gccgacccac ccgcgcgcct
     2761 acggcaccta cccgcgcgtg ctcggcaagt acgtccgcga ggagggcgtc ctgcaactgg
     2821 aagacgccat ccgcaagatg tcgtcagccg tcgccgatcg cctcagcctg cgcgatcggg
     2881 gccagctgcg gaccggcatg atggccgacg tcgtcatctt tgacccggag acggtcattg
     2941 accactcgac tttcaccgag ccgcaccagc tctcgaccgg catccgcgac gtctgggtca
     3001 acggcagccg tgtcctccag aacggcactc acaccggcgc gatgcccggc cgcatcgtcg
     3061 acggccccgg ccgttaattg gccggtggag gcgagaacct gcgatctatt aacaggtcac
     3121 aggctctcgc cgttacgcag gctggtccga ttcggatgat ggatcaaatg accggtgatg
     3181 aagtcgcctg gtgagagggc ccggatcgac tggccgcgac gatgcaggat gcgtcgaaga
     3241 agacccgcgt cgtaccctca acgatcacgt atcgggagtt tcacggccgg atcaaagtca
     3301 aaccagcgcg cgatctggcg tgcttcgtcg tccatttggt cacgttgcag gaacgcgtcg
     3361 atctcttcac gcgtgtattc gcgtagatga atatccggcc gtgtccgcgt cgcccaaagc
     3421 aggtgctcga tgccctgacg aggaatgcgg taggtccggc cgagccgtga cgcccgcaag
     3481 cgaccctgcc ggatatagca atagatagtc tcgcgattga cctgaaggta ttccgccgcc
     3541 tgctctgacg tcatgatctc aggcatctgc tgtgtcgtcg ccatggtccg agattcccgt
     3601 cgatgtacga ctttacgact tcagtcaact tgacaagatt atagacgata catgcttgat
     3661 acgacccatg actaccgcgt cctggtgccc caaagtcgtc gtggataccc gcgcgcagga
     3721 tcgattcaac gtcgtttgga ttcagaatcc agtatcttgc ataagaatgc aatacgcaga
     3781 ataacggact ccaaatagca gaagaacgca atatacggga agttcttgac gaatagcgac
     3841 acgcaaacta ggatgcgcct aggaacctga caactcgatt cgggggcagg taagtgcagc
     3901 gcgcatgggc gttgtattgc cgcacccgtc atcgtctgag gaggagagcg tggggcgcac
     3961 gagtcgagaa ctcgctgacg aaaactttgc gcgttggcag tggttctaca agaagggcat
     4021 gatcgaccgg cgaacgtttg tcaaggccac gatggcctgg tccggcgccg cagcaatggg
     4081 tggcagcctg ctggcagcct gcggcggcga cgacgatgat gacgacagtg gcagcggcgg
     4141 cgccgcgaca accgccagtg gcggtgcgac gacggtcagt ggaaccggtg cccagccgac
     4201 agcgacgacc gcagccggcg cggcgccgac atcgggcgga ggcggtggca caaccgaggg
     4261 tggcacactg cgggtggctc tccagacgtc cgagaaccag ggcatggacc cgcacatgca
     4321 caatcagcgg accggcatta tcttcttcta tcacacgcac gacaacctcg gcgtccgtga
     4381 tatgtcgacg atgcagatcg gcccatggtt ggccgagagt tgggaagtga ccgacccgac
     4441 cacctggaac atgaagctgc gccaggatgt caagttccac aacggcgacc cgttcacggc
     4501 cgagaccgtc aagtggaact gggaacgggt catcaatccc gagcagaaga gccagcaggc
     4561 aggcaaccat gcccagatcg ctggtgtcga tatcgtcgat gagtacacgg tcaacgttcg
     4621 gacgaaggtt ccgtacccga tcttcaccga gcggttgcag aacttccaga tgatccccga
     4681 gaagctcgcc caggagaagg gtgacgcctg gctggccgag aacccggtcg gctccggtcc
     4741 ctacaagctg gtcgagtgga agaagggatc cgagtacgtc ctgacgcgca acgatgacta
     4801 ctggaacaag gacgtcacga tcgcctacaa ggatctgatc ctgcggacga tgccggagct
     4861 ctcgacgcag ctggccgaac tgctggccgg cggcctcgac atcatccgcg tcgtgccgtt
     4921 cgatcagatg aaggtcgtca ccgattccgg cgtcgcgacc ccgaaggtgc aggacatcct
     4981 ccgtgtccag ttcgtcgtcc tcgatgccaa gggccgcggc acacctgaca acccgttcac
     5041 ggatgttcgt gtccgcaacg ccgccaacca tgccatcgac gtgcagacct atatcgacac
     5101 gctccaggcc ggtggcgacc ggaccccggc gctggtcaac ccgaagcact ttggcttcga
     5161 tcccagcatc gtgccgcacg agtacgatcc cgagaaggcc aaggcgctgc tgaaggaagc
     5221 cggttacgaa gatggcttcg atgtcaagtg gaagaccggg ccgcagttga tgccgaacgc
     5281 gacccaggtc gagcaggcca tgcagcgcga tctggccgct gtcggtatca atgccgagtt
     5341 cgagatcgtc gccgatggta gcgtcagcag ccagcagaca aacgagggca agaatccgcc
     5401 gatggcgagc acctcgtggg gttcctactc ggtcttcgat gccgacggca tcctctggga
     5461 catgctgcac tcgacctcga tcttctcgta ctacgagaac aaggagttcg acgcgctgat
     5521 cgacgaggcg cgcggcattc tcgattccga caagcgcaag gaactgtatt cctcggcgca
     5581 gaagatcctg cgcgaggaag caccgatgct cttcatgtgg ggtctgcaca ccgtctgggg
     5641 tgtcaacaac gatgtcgact ggaccccggc tccggatgag atcgaccgct acttctgggc
     5701 caaacccaag gcgtagttcc aaacgacgtg gcaacgggaa tccgcttcca ggcgggctcc
     5761 cgttgtcgca gtcggccagt cgctgctggc gggtccgggc atgaaggggg agcgagccgg
     5821 tggtttccta tatcatcaat cgtctcattc agatggtcgt tgtcatcttc ggcatatcag
     5881 tgatcgtctt cgttatcacc aatatgcttg gcgatccggt cggtgtgctc ctgccgccgg
     5941 aaacaccgta cgagcaacgc gagatcttcc gcgaggagat gggattcaac cggcccctca
     6001 tcgttcaata tggcgacttc ctcactgacg cggtccgtgg tgacttcggc gattcgttcc
     6061 gctcgggacg tcccgcctca acgctggtcc tcgaacggat gccggcgacg ctgacactga
     6121 cgttcaccgc catcctgctg gcgaccctga tctcgattcc gatggggatc atctccgctt
     6181 acaagcgtca ctcggtctgg gagaacttcg cgacgttgtt cgtcgtcacc ggtcaggcca
     6241 tgccgatctt ctggctgggc ctgctcctga tcatcatctt cggcgtgaat ctcaagtggc
     6301 tacctgtctc cggctatggc gacggtggga tcgaccgcct gaaacacttg atcatgcccg
     6361 cgtttgtcct cggcttcttc atcgcacctg tcaccgagcg gctggtccgt tccggcatgc
     6421 tggaggcact ggaccaggac tacgttcgca ccgcccgcgc gaaggggctc gccgagcggg
     6481 tcgtgctctt ccggcatgcg tttcgcaaca cgatgatccc ggttatcacc gtccttggtc
     6541 tccagttcgg acagttgctg ggtggcgcgg tgatcaccga agccgtcttc gcctggcctg
     6601 gggtcggcca gttggcgatc gagtcgattc gtggagcgga ttttcctgtc acccaggcgg
     6661 ccgtcgtgct gctggcagtc atcatctgcc tggtgaatct gggtgtggat ctcgccgtcg
     6721 gcgggctgga cccgcgcatc cgacgctgat ccggaccgag tcaatcggtc ggctcccatc
     6781 cggtgacatg aggaggaacg atggcaactg ccgagataca gccacaggaa tcgatcgccc
     6841 aggcgttcgc gcacaaccga gtccgccgtc gcacgatgaa gagcatgatg gcggccgcct
     6901 ggaagttgcg catgtcggtc gccggcggga tcgtcctgct gatccttgcg ctgatggcga
     6961 ttttcgcacc actcctgacg ccctacacga ctgaggaggg ctcggtccgg gaacgtctcc
     7021 aaccgccggt ctggcaggag ggcggaacgt gggatcatcc gctggggacc gacggcattg
     7081 gtcgcgacta tgcgacacgc ttgctgtacg gtgggcgcat cgcgctctcc gtcggtctgc
     7141 tggcaacgct gatttcggcc gcaatcggga ttacgctggg catggcggct ggatactttg
     7201 gtggctggct tgacgggatc atctcgtcgc tcgtcaacat catgatcacc ttcccgttca
     7261 tcctgctggc gttggcggtg atggccgtcc tcggcaccag cttcaggaac gttgtcattg
     7321 tgctggggat cggtgcctgg ccgatctaca cgcgcgtcat caagttcgag gtcgagcgca
     7381 tcaaggagct cgaatacatg gcggcctcgc gggtgatcgg catcagcatc tggcgctcga
     7441 tgtggcgcca cattgcgccg aacctgatca acaccgtcat cgtgatcggc accgtccagg
     7501 tcgcccggct gatcatctcg gaagccttcc tcagctacct cggtctcggc gtcccgccac
     7561 ccacgccaag ctggggcttc atgctgaatg aatcgatccc gttctcgttc agctggagcg
     7621 acatctggct gccgacgctg ccgggcctgg cgatcttcat cacgaccctc tcgatcaact
     7681 tcgtcggcga cggcctgcgc gacctgatgg acccccgcga gcgcgcctcg ctctaacgac
     7741 gaacgcacaa tcgatctcca atccggtacg ggcggcatcg caatgccgcc cttttcgtgc
     7801 acgcgacgct gggaacaggg cgataatgcg gtatcgcccg taccggattg actcattgtt
     7861 tggtcttgtt gccgcgaacg ttcgcggtac ctctgcatgc cgaccaatcc ttggatcatc
     7921 ccatccaata tcgacgatgc aatatcgatg agccaatata taggctcttg acgagttttc
     7981 aacctgccgc tacgatcggc ccagtcagga tcggtcgaac aattcgaggg cgttgtccgc
     8041 cagtggtcta tctcgatcac cggtggctat ggtcatcgct gaggaggaga gtgcggtgag
     8101 ttcctggaat ccgtcggaga tgaacgacaa gatctacgcc aactgggagc gcttctacaa
     8161 ggaaggccgg atcgaccggc gcacgcttgt gaaagcggcc atggtctggg cggccggcgc
     8221 cggtgcgatg ggcgcgctgg ccgcctgcgg cagcgacgat gacgacgatg acgacggcgg
     8281 cgcggcgtcg accagcgcca gcggcagcgg cggccaggcc gagacgccgg cagccggtga
     8341 gccaacgaaa gccgctggtg gcgcggagcc gaccagcgcc ggcagcggca gcggcgacac
     8401 tgccgatggc ggcacgctgc gggttattct cgaccagtcg gacctgccga cgatggaccc
     8461 gcacatgcac aacctgcgaa ccgggatcat cttcttctat cacacacacg acaacctcgg
     8521 cctccgcaac aaggagacca accaggtcga gccgtggttg gctgagagct gggagaacat
     8581 cgagccgacc gtctgggaga tgaagctccg ccaggatgtc aagttccaca atggcgatcc
     8641 cttcaccgcc gagaccgtca agtggaactg ggaccgggtc accaaccccg agcagaagag
     8701 ccagcagatc ggcaaccacg ccgcgattgc cggtgtcgag gtcatcgatg acttcacggt
     8761 ccatgtcaca accaaggagc cgtacccgat cttcgccgag cgcttgcaga acttccagat
     8821 gatccctgag aagctcgccc aggagaaggg cgatgcctgg ctggcggaaa acccgattgg
     8881 gagtggccca tacaagttcg tcgagtggaa gaagggtcag gagatcgtcg tcacccgcaa
     8941 cgacgagtac tggaacccgg atgtcaagcc ggcctataag gacctcatct tccgaatcgt
     9001 gccgggcgtc ccaacccagc tggccgagct gctggccgga acggcagaca tcatccgcgt
     9061 cgtgccgttc gaccagatga aggccgtcga cgactcagac gttgcggtga cgaagacgca
     9121 ggcgatcctg cgcgtcggct tcacccggct ggacgcgatg ggccggacgg cgccgaatcc
     9181 gttccaggat gtccgcgttc gccacgccgc caaccacgcc tgcgacattc agggctacat
     9241 cgacgccctc cagccgggtg gcgaccggac gccggccttg ctcaacccga tgcacttcgg
     9301 cttcgatccg tcgatcgagc cgcacgagta cgatcccgac aaggccaagg cgctgctcga
     9361 agaggccggc tacccggacg gcatcgacgt cacctgggtg cgcgggccgt cctcgatgcc
     9421 gaaccaggac caggtcgacc aggccatgca gcgtgacctc gaagcggtcg gcattcgctg
     9481 taccttcgag acgctcagtg acggtaacgt cttcacgacc cgtcacaacg agggccaggc
     9541 cgggccgatg cacagctaca actggggctc gtactccgtc ttcgacgccg acggcatcta
     9601 ctgggaccag ctccacagca gctcgatctt cacctattac aacaaccccg atctcgacaa
     9661 gctgctggaa gacgggcgcg gctcgctcga tcccgaggag cgcaagcgga tctactccga
     9721 cgcgcagcgg atcgtccgcg atgacgcgcc gatgatcttc atgtggggct tccactccgt
     9781 ctggggtgtc agcagcaagg tcgaatggtc cccgcgcgcg gacgagatcg acatgtactt
     9841 caccgccagc ccgaaggcgt aggtgacctc cgccagccgg aacgtcaaca ccgcgacgtt
     9901 ccggctggct ggggattgcc tgctgcctca acgtgccggc gtgagacagg aggtcggagc
     9961 cagtggggtc cttcatcgca catcgggtgc ttcagacgat catcgccgtc ttcggcatct
    10021 cggtgatcgt cttcgtcatc accaacatga tcggcgatcc ggtcaacgtc ctgctgccgc
    10081 ccgagacacc acaggagcaa cgcgagatct accgtgagga gatgggcttc aaccgtcccc
    10141 tcatcgtcca gtactgggat ttcgtctcgg atgccgtcca gggtgacttc ggcgtctcgt
    10201 tccgtgccga acggcccgcc atgacgatcg tctgggagcg gatgccggcg acgctgctgc
    10261 tgaccttgag cggcacgatc gtcgcgacgc tgatcgcgat tccgatgggg attctctcgt
    10321 cctacaagcg ctactccatc tgggacaaca tctcgaccct gttcgtcgtc gccggtcagg
    10381 cgatgccgat cttctggctc ggcctgatgc tgatcatcgt cttcggcgtc aacctcaaat
    10441 ggctgccgcc ctccggctat ggcgagttcg gcatcgaccg gctcaagcac atggtcctgc
    10501 cgacgatcgt cctcggcgtc ttcctcgcgc ccgtcaccga gcggctggtt cgctccggca
    10561 tgctcgacgc gctgacccag gattacgtcc ggacggcacg ggcgaagggg ttgcgcgagc
    10621 gggttgtcct gacgcggcac gcgctgcgca acacgatgat cccggtcatc accgttctcg
    10681 gtctccagtt cgggcagctg ctcggcgggg ccgtcatcac cgaaaccgtc tttgcctggc
    10741 ccggcgtcgg gctgctcgcg atcgactcga tccgcaacgc cgacttcccg gtgacccagg
    10801 cggccgtcgt ctcactcgcg atcatcatcg gtctcgtcaa cctgggcgtc gacctcgtgg
    10861 tcggcatgct cgatccacgc atccggcgct gatatgcctg tcacctggcg gaccgccatt
    10921 ccgactggtg acaccgtacg ctgatgagga ggggtaatgg cgacggctga cccaacgatc
    10981 caggacgccg cttcggcgtt ctcaccgcag cgaacgcgta agaagacgtt ccggcagtgg
    11041 atgcagacat tctggaagct gcggatgtcg gttgccggcg cgacgatcct cgcgatcctc
    11101 atcttcatcg cgatcttcgc accgttgctg acgccgtatt cgtacacaga gggacgcatc
    11161 accgagcgtt tgcaggagcc ggtctggcag ggcggcacct ggtcgcaccc gcttgggacg
    11221 gacggcatcg gccgcgacta cgcgacccgg ctgatgtacg gcgcgcggat cgccctctcg
    11281 gtcggcctgc tggcgacgct gatctccggc gcaatcggca tcacgctcgg cctgctggct
    11341 ggcttcttcg gccgctgggt cgatgggatc atctcgacgc tcgtcaacgt catgatgacg
    11401 tttccgttca tcctgctggc gctggccgtc atcgccgtgc tcggcgcgag cttcaccaac
    11461 gtcgtcctcg tcctcggcgt cggtgcctgg ccgatctaca cacgggtcgt gacgtttgag
    11521 gtggaacgga tcaagtcgct ggagtacatc gccgccggcc gggtcgtcgg actggggaac
    11581 atgcggctga tggttcgcca catcatgccc aacctgttca acacgattat cgtcattgcg
    11641 actgtccagg tcgcgcggct gatcatctcc gaggcgtttc tgagcttcct cggcctcggt
    11701 gtcccgccac cgacgccaag ctggggctac atgctgcagg agtcgcaggc gttcatgttc
    11761 acctggggcg atctctggct gccgacgctg ccgggtctgt tgatcttcat cactgccctc
    11821 tcgatcaact tcgtcggtga cggactgcgt gacctgatgg atccgcacca gcgcgcctcg
    11881 ctctagtcag accacgtagc gaccggcctg cttgcgccac acatttgtgc tgcaacgcag
    11941 tgcgcaagtg tgcgcggaga ctgtatcctc taagcgtggg cgcatcgatc atgcaattgc
    12001 cgtatgatcc accgaggctg cggaatctgg ttggatggtg gaccaggcta tgttgcgctc
    12061 gggcgctctt cagtcgcccg acgtcggcga tcgttgtttg gatggcaaga cgggcgaatg
    12121 caggtaaccg atctccacgg cacccccttt gccgatacat acggtcaggt gctgcgcgac
    12181 tatcttcaga ctcgtcgcga ggacgcgctc tatcgcgctt cgctgctgag ccagacgttc
    12241 gtcgagcgtg gggtcggccc ggaagagatc atcgcgctcc actttgaaca actcgaccag
    12301 gccctcgacg gactctccta ccgtgtgcag gcccgttcga tcggcgacgc ccaccagttt
    12361 ctgcttgaag tgatgatcac ctatggtgtc cactacaaag agtatctcga gctccggatg
    12421 cgggaaggag tccaggctgc tgaagcggag gccgaactag agcggcggct tcggcaggaa
    12481 cgcgagcgga ttgatcagga acgaaccgag ctgcttgagc acatcgcgca cgagttgcgg
    12541 acgccgatta cgacagcact gggtacgctc gatctggcca gacgatcaat cgcacggggg
    12601 aacttccatc ggcttgagcc gctgatcggc acggcccgtg aggcagtcga tcgactgtca
    12661 cgcctgagtg cccagctcgt ccagatcagc catggcaaac ttcccgatct ggagcggaag
    12721 ccgcaggaca ttagcgcact gctcacgcaa gcgtgtgcgt gggcggaagc gtctgcggtg
    12781 gagaagcagc ttcaatttca gtgcggtccg ttcaatgaac atgtctatgt tatcggcgac
    12841 gccgacgccc tgctgagcct gttcgggaac ctgctgtcca acgccattcg ctatacaccc
    12901 gttggcggca ccgtctcgat gacctgtcac gtcgaagggg agacggtctt catcgagtgc
    12961 cgcgataccg ggatcgggat gtcccacgac gtgcaggcgc ggatcttcga gcggttcttc
    13021 cgcgcgccag aagcacgcaa cgttgacgca cagggtctgg gagtcggtct gtccctcgtc
    13081 catgcgctcg tccaggccca cgacggggcg atcagtgtta gcagtgtcac aggcgaagga
    13141 agcgtcttcg gtgtcacctt gccggtcatg cgggaaggtg acggtcacgg cgcggaactc
    13201 gcgttcgatg agatgtcgga gtcgacggag gagacgacca attgagcgag aatgttgtcc
    13261 ggcaacagca atcgaagctt gagcagcaac gaagaacgat cgccgaactc cagacaccgg
    13321 tcatccaggt ctggaagggc atcctggcgc tgccgattgt cggcagcctc gacaccgtcc
    13381 gtgctcagga gatgatcgag gccctgctcg accgtatcgt cgagaccaac tcagatgtcg
    13441 tcatcctgga catcaccggc gttccagtcg ttgacacggc cgtcgcgaag cacctgctcg
    13501 aaacggtcgc ggcggctcgg ctcctgggga ccgaagtgct gattgttggt ctgtcgaccc
    13561 ggactgcgct gaccctcgtt cagctgggta tcgacctctc acacgttaca acgcggacga
    13621 cgctcgcgaa gggtctggaa ctggcgtttg accggctcgg gctggaggtc gtttcgaagc
    13681 ggcgcgaatc gcccgcaagg gaggctgacg aagtgtgaca cgcgtcccga ttgtcccgat
    13741 cgacgatatc ctggttgcga ccgtcctcga agatctgtcc gatcaggacg ctattcggct
    13801 tcaacacgac ctctgcctgc agatcgaacg caacggcgcg gttggagtcc tgctcgacct
    13861 gtccgtcgtc gagatcgttg attcctttct cggccgtctg atcagcgaag tcgtcgccag
    13921 ctcgcggttg ctcggtgccg agaccgttgt cgtcggtatt cagcccgcgg tcgcgattac
    13981 cctcgttgag ctggggctgg atctgcgagg tgtccggact gccctcactc caaagaaggg
    14041 gcggcggttg ctgaatcaac tcatcgcagc gtcaagatcc ggcatccgcc atggccactg
    14101 aggagcgcat tgacctcgtc ggtgaagaga gcatcgtcgt tgctcgccgg cgggcgcgag
    14161 agtatgtgcg cggtctcggc tttggactac tcgatcagag ccggattgcg acggcggtct
    14221 cggaattgac gcggaacgtc attcgctacg cgatcgacag ccgtggcgaa gtcgtcattc
    14281 gcgaacttcc cgaatccggg acgcggatcg gggttgagat cgtcgtcagt gacgccggtc
    14341 caggaatcga tgacgtttca caggcgatgc gcgacggcta tacctccggt cgcggtctgg
    14401 ggctcggcct tccgggaaca cggcggctag tcgatgagat ggagatcgat tccggacgcg
    14461 ggcgtgggac gacggtcacg atccgcaaat ggcgtccttg acgtggagtc cgtgattaca
    14521 atcgatatcc gaacccgacc agatgttgac ctcgcacgcc gagaggttcg ccggcttgcc
    14581 gcctcagccg ggctcaactt tcatgacgtt gagatggttg ccctggctgt caccgagttg
    14641 gcgttcaacc tcgttcgcta tgcccagggc ggaagcatcg tgctctcccg cgcgatcagc
    14701 gagggccgaa ctggtgtgca gatcgagagt cgcgacactg gtccaggcat tgacgacctg
    14761 gtccttgcga tgcaggacaa ctacagcacc ggcggcgggc tcggcagtgg cttacccgcc
    14821 gtcaagcgtt tgatggacga attcaccatc agttcgactc cgaaagggac ccgggtgatc
    14881 gcacgcaaat ggatgactcc caatcgccgc tgatcattgc aatcgcatcc cggccacatc
    14941 cgcacttctc catcaatggc gatcagtcca gcgtccactg gcgtgatggc gcatgcgtcg
    15001 tcaccgtcgt cgacggactc ggccatgggc ccgaggcggc cgacgccgcg tctctcgcga
    15061 tcgaatcact ggctgtggga gccgatttgt cgcctggcga ggcgatcacc cgctgccacg
    15121 atcggttgtt caggtcgcgt ggagccgcca tggcggtcgc gcgggtcgat ctcggcgcgg
    15181 ggcaactaac cttcgccggc gtcggcaatg ttgaggctca gttctggaac ggaacgcgtg
    15241 tccagcggcc gatcgcgcag cgtggaatcg tcggtgcgac gctgcccagc attcgcgagc
    15301 aggtgatcga actcaacgac gcgggctggc tgctgttgat gttcagcgac ggaattcacc
    15361 agcgcttcga tctggagctg ctgcccgagt tcgtcgcccg cgacgcacag acgctcgccg
    15421 atcgcatctt gagcgattgg agccgcgaaa ccgacgacgc gaccgtcctc gtcctcatgc
    15481 cgcgtgtcac gcagtaaccg cgtagctgac gcggcctacc actcgtggtc cgtgtagtcc
    15541 ccgcgcatca cgttctggcc ggttggggcc tcgctgccgc cggctggttc ttccgtgaca
    15601 ccacagcgcc agccgggggc gtaatcggcc ggggcttcga tgacgacgat cgcctggccg
    15661 ctggagtcga cgtcgaagac accgccgtcg tggcgcgttt cgtccggctg gacaaaccag
    15721 agttggtagg cgcgatcgtc cggtagcggc ggcaggccgc tgacgaccag cccggctgtt
    15781 gtctcaccag gctggaagaa gagctgggcg acggcgtccg gcgccgcgtc agatcccagc
    15841 atcgggatga tctgcgtgtc cgggctttcc tgaagctcgg cgacgactgc ggcggcgttg
    15901 ttggcctcgt catggttgcc cagcgacgag aacagtgccc cgaccacgat tgcgacgatg
    15961 atggcgaccg tcgccagcgc cggcagccgg aatcggggcc accagccgtc cgccccgcgg
    16021 cgcgttgcgg cggtgtcggt ccggacccgg ttgaacagct cccggcgggc ggccggcgac
    16081 ggttcggagc gcggcaggcc gagcggcagg agccgcatga cctcttcata ctcgacgagc
    16141 agccggcgac agtcggcgca ctcaccgagg tggcgttcca ggcgcgtggt tgccggcggt
    16201 tcgagcgtgc cgagtgcata gctgggcaaa tcatcttgat aggtctcgtg cggaatgctc
    16261 attgccggat ttcactctcg tcgtcgggtt tgaagtgcgg gcggagcgcc gtccgcaagt
    16321 gttccatcgc gacacgggtg cgcgatttga tcgtgccgag tggttcgccg agctggcggg
    16381 cgatctccga ttgcgacatc ccgccgtagt aggccagctc gacaacctgt cgctgtgcca
    16441 tcgtcagccc ggacagcgcg tcggcgacgg cctggcgggt cgcgaccgcc tccgtctcgt
    16501 cgggaacgcc gaagctatcc tcttcgcccg gctccggcag cggcgtccgc tcgcgcatac
    16561 gcgccttgtg ctgccggctg cgcaggacgt cgatggcccg gttgcgggcg attccgatca
    16621 gccagcctga tacctgaccc cgcccggcgc ggtaggtttc ggacgcgttc cagacgcgca
    16681 ggaaacagtc ctgcatcact tcttccgcca gatcccggtc tccaacgaca cgcagcgcaa
    16741 cggtcaggat cagcccgcca tagcggtcgt agagcgccgc cagcgcggct tcctcccgcc
    16801 gccggacacg ttccatcagc tcggcgtcgg cgagtgcgct cactctggcc gcgttgagcg
    16861 ccgaatgttg tccggcccga tcgtccggga cggtcacgaa gaatgccggc accgcttgct
    16921 cccaccccgc ttcgttactc gtgtcgcggt gtactacgca ctgacgaccg tgaatggatg
    16981 agtcagggta tctgcgttac cgtagcatgt gcgaagaggt gtcgaagcac aggaggagag
    17041 cgtgtcggtg cagccgcaat tgctggtcgt ggatttcgcg ggaaccatgt ttcgtgaaga
    17101 aggtgctgtc gtcgtcgcct atcgccaggc gttcgccgcg ttcggaatcg acgccacgga
    17161 cgacgacatc gccgcccggc gcggcgccag caagcgcgcc gtcttcgagg agctggcagc
    17221 ccgcaaccgg ccacgtgccg aagccccggc aatcgccggt cacgcactgg ccgccttcga
    17281 ggaggcgctg cgggccgagt acagcaccgg tcccgttcag gaggtgccgg gcgccggcga
    17341 ggccgccggc cgcctgcgag ctgccggcat caaggtggcg atcgccagcg gcttcgaccg
    17401 gccgctggtc gatctgctga tcgcccggct cggctggcac gacctgttcg acctgacgat
    17461 cgccggcaac gatgtcgccg ccggccggcc gtcgcctgcc atcatctacc acgcgatgac
    17521 gcggctcggc gtcaacgacg ttggcgcggt cgccgccgcc ggcgacaccc cgcttgacct
    17581 ccaggccggc actaacgccc gcgccggctg ggtgatcggc gtcctcagcg gcgcgcacgg
    17641 catcgacacg ctcggccgca cccgccacac ccacctgctc gcgagcgtcg cggagttgcc
    17701 ggagttgttc agcgtgtaaa gagattcgcg tctcgcgtag gggcgatggc cgcagcctcg
    17761 ccctgcttgg atcgcacgat
//
LOCUS       DASUGJ010000629         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1075654, whole genome shotgun sequence.
ACCESSION   DASUGJ010000629 DASUGJ010000000
VERSION     DASUGJ010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075654"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..622
                     /locus_tag="VFV93_14395"
     CDS             302..622
                     /locus_tag="VFV93_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166590.1"
                     /translation="MSIEPAELTSDRTRIWPSTESASIDDHYRVAAFPADYALTNDVI
                     LRDETGRAFLLSQADEELLPIEEDTANSLGMFFEPSQDSSWHTVSELRRIIYGLDDTV
                     VSIH"
     gene            complement(708..1079)
                     /gene="erpA"
                     /locus_tag="VFV93_14400"
     CDS             complement(708..1079)
                     /gene="erpA"
                     /locus_tag="VFV93_14400"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX5166591.1"
                     /translation="MTMITITHEAVDQLKDFLADQGTPEHALRVFVAPGGCSGLQYGM
                     SIEEAEDEGDTVVTTEGVKLLVDEFSAMYIAGAEIDYVNSLMGGGFTVRNPNAVTGCA
                     CGHSFDTGMNAGTSQGCGCGS"
     gene            complement(1188..2000)
                     /locus_tag="VFV93_14405"
     CDS             complement(1188..2000)
                     /locus_tag="VFV93_14405"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEX5166592.1"
                     /translation="MAALLGVSWSPVAGELSDLGLLVDIIEVPGWSLESALAPDGVRR
                     RALLHNLDLDVSLTDPGYIDDEWGERANRAIQRAGTPWFSLHLGFASERVRFDEHMLP
                     ESEPLTRDVVLQRTVESVRRAKAYLDLPLLLENLDYCPEGAYEHICEPAFISDVLEAT
                     DCGLLLDIGHLLVSASWLETDPEAMLAQLPLDRVVEVHLSGPRPLAGNIERLDDVHEV
                     VSEREVQLLRLVLEQAQPKAVVLEYRRDAELLREQLAMLGRVIGRRWRGSPC"
BASE COUNT          409 a          728 c          554 g          395 t
ORIGIN      
        1 tcctgcgcaa gctcgaagtc accgatcgcg tccaggccgt actgttcgcc gtcaagcatg
       61 gatgggtcga gatcggctcg ctacagccgg gcgatattcc cgacgacacg cgcaacctcg
      121 aagtcgaata gcgtcgtcaa ccttgggctc tctttgtcag ttggccttaa ggcgattgtt
      181 gcctctaagt caatgctggc atgaatcttg ctgatccagt acctgccaac gacgcgaacc
      241 tcctcgcgct cggtgtaggg cctgggtgaa tagctcctga cacggaggaa ccggaatgtc
      301 tatgtctatt gaaccggcgg agttgacttc cgaccggaca cgaatctggc cgtctaccga
      361 aagcgcatcg atcgacgacc attaccgagt cgccgccttt ccggcggact atgcgctgac
      421 caacgatgtc atcctgcgcg acgagacggg ccgcgcgttc ctgctctcac aagccgacga
      481 agaactgctg ccgattgagg aagacaccgc caactcactc ggcatgttct tcgagccgtc
      541 gcaggactca tcgtggcata ccgtttcaga gctgcgccgg atcatctacg gcctggacga
      601 caccgtcgtc tccatccact gatccacacg actgccagaa ggtgcacagc aaagcggggc
      661 gcgattcgcg ccccgcttct tcgtgtctgg ttgcttggcg tcgggcttta cgacccgcag
      721 ccgcagccct gcgacgttcc ggcgttcatg ccggtatcga acgagtgccc gcaggcgcag
      781 ccggtgactg cgttcgggtt gcgaaccgtg aagccgccac ccatcagact gttgacgtag
      841 tcgatctcgg ctccggcgat gtacatcgcc gagaactcat cgaccagcag cttgacgccc
      901 tcggtcgtca cgaccgtgtc gccctcgtcc tcggcctctt cgatcgacat gccatattgc
      961 aggcccgagc agccgcccgg cgcgacgaag acacgcagcg cgtgctcagg cgtgccctgg
     1021 tccgccagga agtccttcaa ctggtcgacc gcttcgtgag taatcgtaat cattgtcaat
     1081 gcttccttct gcgtccgtcc gtcggagcac ccttgcccgg ttcgttacga caagtatact
     1141 tccgcaacgt ctctatttca taactatgac gcccgtcggg tcacctgtca acaggggcta
     1201 ccgcgccagc gtcgcccgat caccctgccc agcatcgcga gctgttcacg cagcagttcc
     1261 gcgtctcgcc ggtattccag cacaactgct ttcggttgcg cctgctccag caccaatcgc
     1321 aggagctgta cctcgcgctc gctcacaact tcatgaacgt cgtcgagccg ctcgatattc
     1381 cccgccagcg gtcgcggacc actgagatga acctcaacaa cgcgatccag cggcaactgc
     1441 gccagcatcg cctccgggtc ggtctccagc cagctcgcgc tcaccagcag gtgtccgata
     1501 tccagcaaca acccgcagtc ggtcgcctcc agcacatcgg aaatgaaggc cggctcgcag
     1561 atatgctcgt aagccccttc agggcagtag tccagattct ccagcaacag cggcaggtcg
     1621 agatacgcct tcgcccgccg gacgctctca accgttcgtt gcaacacgac atcccgcgta
     1681 agcggctccg attccggcaa catgtgctca tcgaagcgca cccgttcgct ggcgaatccc
     1741 agatgcaggc tgaaccacgg cgtccccgcc cgctggatcg cgcggtttgc ccgctcgccc
     1801 cactcgtcat caatgtaacc gggatcggtc aggcttacat ccaggtcgag gttgtgcagc
     1861 aatgcccgcc gccggacacc atccggcgcc agcgcactct ccagactcca accgggtacc
     1921 tcaataatgt caaccagcag cccgagatcc gacaactcac ccgcgaccgg cgaccacgac
     1981 acgcctaaca acgctgccac atttacccca catcagaact ggcgctacga catgaaactc
     2041 gggcgaggac cgcacccctt gccctgtcat cctgagccgc agcgaa
//
LOCUS       DASUGJ010000630         9679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_102209, whole genome shotgun sequence.
ACCESSION   DASUGJ010000630 DASUGJ010000000
VERSION     DASUGJ010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102209"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VFV93_14410"
     CDS             <1..784
                     /locus_tag="VFV93_14410"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5166593.1"
                     /translation="TDLLAFDGYPSYSKIIASVALTFFAYLGFNVITFTGGDLRNPRR
                     DIPRATYLSLGITATIYILIAIGVFGTLTVTQVIDYGETAIAEAARPTLGDAGFYIMA
                     IVALLSTAGATNATLYASSNLTNMLASEGLFPEFFGSRSRFGPRSGLIITTLLVLVVA
                     NFVDLSSIASVGSAVSLIVFMLVGAAGYQRRKETGSNTVIVVLAIAMTGIVLTFFAID
                     ILQNDMGSFIAIIGIALVSVVLDTVFRHRVVEPEPVPATSQP"
     gene            815..1177
                     /locus_tag="VFV93_14415"
     CDS             815..1177
                     /locus_tag="VFV93_14415"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX5166594.1"
                     /translation="MLRVLEDLQANVLGVEAVGIVTEADYERVLIPAIRAKRDAHEKL
                     RFLYLLGEEFDGWSMGALWEDTKLGLTSASAFEKIAVVSDNVSVHHAVKAFGWMIPGD
                     VRVFDDDQLDDAKDWVAS"
     gene            complement(1211..2563)
                     /locus_tag="VFV93_14420"
     CDS             complement(1211..2563)
                     /locus_tag="VFV93_14420"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEX5166595.1"
                     /translation="MHDALTASIATTTVVNDIDSKAYETVQFLTRLGVAMTVAGASVT
                     EVQNSLTQSAGAYGLRSNITVGPTMILVQVQGQHSVSLDMSSAMQRSLRLDQVAGIWR
                     LVERAMAGTIQPAQGLTQLSVIWGSTSRFAESIRLLGYMLMAVGFGLSLHPSTGVVIA
                     CILFGLVVGEILLLTEGHATIRVLLPVMTALGVGLLVFLAVDYGLASGPLLLLIPPLV
                     TFLPGAMLTTGMMELTSGDIVSGSSRLVAGMVQLVLLLFGLLIAASIVGLPTAEAFST
                     VGPSNFWSWGPWAGTFIYGIGIYLHRTAPARSLPWLLLVLLVAQAGQAAGSAIAGGYL
                     GGFFGALAMTPVAYAIQRWPSAPPAMVSILPAYWLLVPGALGLMKLTELVSETSQAAI
                     NDIGKTIYTLIAIALGVTVGQSVYVPLASWSTRVAGRIARGRTRPDPSGDASLPGAGQ
                     "
     gene            complement(2602..3435)
                     /locus_tag="VFV93_14425"
     CDS             complement(2602..3435)
                     /locus_tag="VFV93_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166596.1"
                     /translation="MDEFGPIQLIVIGFDSLDRFKGEIADELDAVRSRGVIRLIDALA
                     VARNAAGDVVAIEDSDFDLDEVANLGTAIRGLVGLNGHTNGVTSADASSRGIGLTSED
                     VRHAAEALEPGTGALFLLIEHTWATGLKYAVRNAGGVPLVQGFLTPEAIMMVGAELRA
                     IVDAEVTIELAEAVKGAAILDALITMEAAAEAAEDAMRVADEVIDITDAIRAQAAAEA
                     VRALIVAELIEDAAAEEAAAVLVAAGLIEAAALAEAAEVALAVETELQAAATPDAAAG
                     A"
     gene            complement(3477..3962)
                     /locus_tag="VFV93_14430"
     CDS             complement(3477..3962)
                     /locus_tag="VFV93_14430"
                     /inference="COORDINATES: protein motif:HMM:NF016413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEX5166597.1"
                     /translation="MGSAYRMRERLLAFGDDFYIESGRGERVFWVDGKALRLRDTLSF
                     KDMRGNEIYRIQEKMLRIRDSMTVYRGNDVAARVHKALITPLRDRYKVEIPGSPDMST
                     QGNILQHEYRIERDGTPVATISKRWFRIRDTYGVDIAPGQDDLLVLAITVCIDAMSQV
                     G"
     gene            complement(4119..4418)
                     /locus_tag="VFV93_14435"
     CDS             complement(4119..4418)
                     /locus_tag="VFV93_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166598.1"
                     /translation="MPDEGFQAGTVAKRRSRCDMLRLLQFVQQFPISRWRRLGNDQAM
                     VVWLIVLTAYVALGLQLLAAWVWIRFQRIVFADNAVRTVTSRPGKQQSSGRAATN"
     gene            complement(4497..5411)
                     /locus_tag="VFV93_14440"
     CDS             complement(4497..5411)
                     /locus_tag="VFV93_14440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEX5166599.1"
                     /translation="MAVSDEIIRQMRVEPGTPANIRGRDAAWRGEEYYPGLDSEELMP
                     LAKQRLDEFVEELAHAQKRLWADDTYALLLVFQALDAAGKDGTIKHVMTGVNPQGCQV
                     FSFKKPSSEDLDHTYLWRYSKGLPERGRIGIFNRSYYEEVLVVRVHPEILQHQKHPPS
                     AVGPNMWEERFEDINAFERHLDRNGTKILKFFLNVSWDEQRNRFLRRIDNPDKNWKFS
                     SGDIAERQFWDQYTDAFEDALTKTSTPWAPWYVIPADHKWITHAVVGGIIVNAIESLN
                     LSYPQATAEQIAGLSAARERLTSERRPG"
     gene            complement(5542..6348)
                     /locus_tag="VFV93_14445"
     CDS             complement(5542..6348)
                     /locus_tag="VFV93_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166600.1"
                     /translation="MTATATEGTAVTENGRQRMSDADLVTLMGLIKGAKTVELKLTIP
                     DDARYTTLQSLEVDPLDAEIRQVMFFDTPDLALNQAGVVVRARRVQVKGGDTVIKLRP
                     VVPDQLPPDIRNLPNVGVEVDAMPGGYVCSASYKGQADDVNIKAVMAGQHPVRKLFSK
                     EQRAFYTTHAPTGLALDGLAMLGPITVFKLKFNPKGFSRKMVAELWNYPNGQRLLELS
                     TKCAPNETFQVVAEVRAFLHNRGISTGSVQQTKTKTALDYYSNLLKTSKT"
     gene            complement(6635..7534)
                     /locus_tag="VFV93_14450"
     CDS             complement(6635..7534)
                     /locus_tag="VFV93_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5166601.1"
                     /translation="MASTSPSLGNPAEMPLYILGVVVNVVIVAAIVTNYWWLEDLPGA
                     ISDGVTSDAIQYAMYALLLLIPAIVIGRQVQRASVLGQAVQLSPDQFPDIYAVKDDYA
                     RRLGLRRDPEIYLVNGNGTLNAFASSSVGRDYVVISNELFANLYDNNREGLAFIIGHE
                     LGHIKRNHTKIWYQLSILFFSMLPVISYCLSRAREYTSDRHGAWLSPNGVDGLVLLSC
                     GRYAYHYANVSEVLHQEQQVRGVWVELVTIFRSHPLTIRRIRKLIDLGLMSPDRPSPV
                     PAASPVTSAANTVPVRRYPVHGE"
     gene            complement(7572..7952)
                     /locus_tag="VFV93_14455"
     CDS             complement(7572..7952)
                     /locus_tag="VFV93_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166602.1"
                     /translation="MEKTSTHDWVRPYLLPIVVMIAITTAVVLLFDESVSGFIAAACC
                     WAVAFVVGYALRPVHPWVTPLVLVVVGVIASYIAAEVGDYDPPGGIWVFIGLVIMAGL
                     PLTFLIWIGREVGLTREEMKPLSH"
     gene            complement(8148..8654)
                     /locus_tag="VFV93_14460"
     CDS             complement(8148..8654)
                     /locus_tag="VFV93_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166603.1"
                     /translation="MIRHAMLMMVVVLLAVSLTGCGGDDDDDDATDSSGTTATTGTTS
                     DAAGTEGVADSLYTTAELEQFGTDLVNAVASGDKDALTATLSGVVPQSRIDELAACKP
                     ADLETSNVFVSVIVEPPVMKMSGTVDVTTGGDTQTKVVNWQTELSEVSPGVYTFSSLG
                     EGCPFIFQ"
     gene            complement(8827..9120)
                     /locus_tag="VFV93_14465"
     CDS             complement(8827..9120)
                     /locus_tag="VFV93_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166604.1"
                     /translation="MVLTDTDMDLVVASPIHSNGLVAVYSLGQTPAGEWFAIGSVRST
                     VVALNHPAWIIVSTGTSKEATVEALQAQLEVESRRLISWDLPDPFGGQGAAAD"
     gene            complement(9337..>9679)
                     /locus_tag="VFV93_14470"
     CDS             complement(9337..>9679)
                     /locus_tag="VFV93_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5166605.1"
                     /translation="HARGVQVEAVRQLLAASPFDADEDQWNRRLGPARPLTLRELSII
                     RLLAVGLTVEELSDILSVSVDAFKLDISRLFEKLGVTGRTAAVHRARELGLLHARQPD
                     SPPMINEAAKG"
BASE COUNT         1806 a         3082 c         2955 g         1836 t
ORIGIN      
        1 cacggacctg ctggcgtttg atggctaccc ctcgtactcg aagatcatcg ccagcgtggc
       61 actcacgttc ttcgcctacc tcggattcaa cgtgatcacc ttcaccggtg gggatctgcg
      121 caatccgcgc cgcgacattc ccagagcgac ctatctctcg cttggaatca ccgcgacaat
      181 ctatatcctg atcgcgattg gtgtgttcgg gacgttaacg gtcacccagg tgatcgacta
      241 cggagagacg gcgatcgctg aggcggcgcg accgacgctc ggggacgccg gcttctacat
      301 tatggcgatc gtcgcgctgc tctcgacggc gggtgcgacc aacgcgaccc tctacgcgtc
      361 gtcgaacctg accaacatgc tcgcgagcga gggactcttc cccgagttct tcggctccag
      421 gtcacgcttt ggccccaggt cggggctgat catcacgacg ctgctcgtgc tcgtcgtcgc
      481 gaacttcgtc gatctctcat ccatcgcctc agtcggcagc gccgtctcgc tgatcgtctt
      541 catgctggtt ggcgcagccg gctaccaacg ccgcaaggag acgggcagca ataccgtgat
      601 cgtcgtgctc gctatcgcga tgacgggcat cgtgctcacg ttcttcgcca tcgacatcct
      661 ccagaacgat atggggagct tcatcgcgat catcggaatc gccctcgtct cggttgtcct
      721 tgataccgtc ttcagacatc gagtcgttga gcccgagccg gttccggcca cctcgcagcc
      781 gtagcgcgcg tcgtcgtgac gcgaggagaa ctctatgctc agggtactcg aagacttaca
      841 ggcaaatgtg cttggggttg aggcggtcgg catcgtcacg gaggccgatt acgaacgtgt
      901 cctgatcccg gccatacgcg ccaaacgtga tgcgcacgag aagctccggt tcctgtacct
      961 cctgggtgag gaattcgatg gctggagcat gggtgcgctc tgggaggaca cgaagctcgg
     1021 gctgacgagt gcaagcgcct ttgagaagat cgccgtcgtc agtgacaacg tgtccgtgca
     1081 tcatgcggtc aaggcgttcg gctggatgat ccccggcgac gtgcgagtgt ttgacgatga
     1141 ccagctcgac gacgcgaagg actgggttgc cagttgaggt ctgcgcgtca cgtacctgcc
     1201 ccgcaagatg tcactggcca gcacctggca acgacgcatc gcctgaagga tctggtcggg
     1261 tacgacccct cgcgatgcga cccgcgacgc gagtagacca gcttgcgagt ggcacgtaga
     1321 cagactggcc gacggttaca cccagagcaa tcgcgatcag ggtgtagatg gtcttgccga
     1381 tatcgttgat cgctgcctgg ctcgtttcgc tgaccagctc ggtcagcttc atgagtccca
     1441 acgcccccgg cacgagcaac cagtaggcag gcaggatact caccatcgcc ggaggcgcgc
     1501 tgggccagcg ttggatcgcg taggccaccg gcgtcatcgc cagcgcgccg aagaaccctc
     1561 cgaggtatcc accggcgatt gcacttccgg cagcctgccc cgcctgagcg accaggagca
     1621 cgagcaacaa ccacggtaac gatctggcgg gcgccgtgcg gtggagatag atgccaatac
     1681 cgtagatgaa tgtgccggcc cacggtcccc agctccagaa gttggatggc ccgacggtgg
     1741 agaaggcctc ggctgtaggg agcccgacga tcgacgctgc gatcagcagc ccgaacagca
     1801 gcaggaccag ctgcaccatg ccagccacca gcctgctcga tccggatacg atgtcgccgg
     1861 atgtcaactc catcatgccg gtcgtcagca ttgcccccgg caggaaggta accaacggcg
     1921 gtatgagcag gagtagcggg ccactggcga ggccataatc caccgccagg aagacgagta
     1981 gcccgacgcc gagtgccgtc atcacgggca ggaggacgcg tatcgtggcg tggccctctg
     2041 tcagcagcag aatctcaccc acaaccagtc cgaacaggat acaggcaatc acgacgccag
     2101 ttgatgggtg gagcgacagg ccgaagccca ccgccatcag catgtagccc agcagcctga
     2161 ttgattcagc aaagcgcgag gtactgcccc agatgacgct gagctgcgtg agtccctgcg
     2221 ccggctggat cgtgccagcc attgcccgtt cgacgaggcg ccagataccc gcgacctgat
     2281 cgaggcgcag ggagcgctgc attgcgctgg acatgtccag cgaaacggag tgctggccct
     2341 gcacctgaac gaggatcatc gtcggcccga ccgtaatatt cgaccgcagc ccgtacgcgc
     2401 cggccgactg tgtcagcgag ttctgaacct ccgtcaccga cgcgccggcg acggtcatgg
     2461 ccacaccaag ccgcgtcagg aactggacgg tctcatacgc tttgctgtcg atgtcgttca
     2521 cgacggtcgt cgtggcgatt gacgctgtca acgcatcgtg catcgcgcgc tcgctcaagc
     2581 gatgctgaca accatccagg attacgcacc tgccgcggca tcgggcgtcg ccgctgcctg
     2641 tagctccgtt tcgactgcca gcgccacttc cgcagcctcg gccagcgccg cggcttcgat
     2701 cagcccggcg gcaaccagca ctgccgcggc ctcctcggcg gctgcatcct caatgagttc
     2761 ggcgacaatc aacgccctga ccgcctcagc ggccgcctgc gcccgaattg catccgttat
     2821 gtcgatcact tcgtcggcga cacgcatcgc atcttcggca gcctccgccg ccgcttccat
     2881 ggtaatcagc gcgtcgagga tcgcggcgcc cttgacggcc tcggccagct cgatcgtgac
     2941 ttcggcatcg acgatcgcgc gaagctctgc cccgaccatc atgatcgctt ccggcgtcag
     3001 gaagccctga acgagcggaa caccaccggc gttgcggaca gcgtacttca gcccggttgc
     3061 ccaagtgtgc tcgatcagca ggaacagcgc cccggtgcct ggctcaagcg cctcggcagc
     3121 gtggcgtacg tcttcagagg tcaagccgat cccgcgggac gacgcgtcag ccgacgtcac
     3181 gccgttcgtg tggccgttca atcccaccaa cccgcgaatg gccgtgccca ggttggcgac
     3241 ctcatcaagg tcgaagtcgc tgtcctcgat ggcgacgaca tcgccggctg cgttccttgc
     3301 gaccgcgagc gcgtcgatca gccgaatgac cccacggcta cggacggcgt cgagttcgtc
     3361 agcgatctct cccttgaacc ggtcgaggga gtcaaatccg atcacaatca gttgaattgg
     3421 tccgaactcg tccatcacgg catcctttcc ggatagatca tgggacagtg caggcgttag
     3481 ccaacctgcg acatggcatc gatgcagacg gtgatggcga gcacgagcag gtcatcctga
     3541 cccggtgcga tatcgacacc gtaggtgtcg cggatgcgga accagcgctt ggagatggtt
     3601 gcgaccggtg tgccgtcgcg ctcgatgcgg tactcgtgct gcaggatgtt gccctgagtc
     3661 gacatgtcgg gcgatccggg gatctcaacc ttgtagcggt cgcgcaaggg cgtgatcagc
     3721 gccttgtgga cacgggcagc gacgtcgttg ccgcgataga ccgtcatcga atcgcgaatg
     3781 cgcagcatct tctcctggat ccgatagatc tcgttgccgc gcatatcctt gaacgagagg
     3841 gtgtcgcgca ggcggagcgc cttgccgtcg acccagaaga cacgctcgcc tcgcccggac
     3901 tcgatgtaga agtcgtcccc aaaggcgagc aggcgttcgc gcattcgata ggctgaaccc
     3961 atctgccctg ccatgccggg cgccgagcca cctgatgggg cagccgcccc gcctcgcgcc
     4021 tgcgccctcg ccgcgcgtcg catacctaag cgtggcacat tgataccctc catgatgctg
     4081 atcggtttca tccggtacgt cgagttaccc gggtcgggtc agttcgtggc agcgcgaccg
     4141 ctgctctgct gcttgcctgg ccggcttgtc acggttctga cagcgttgtc cgcgaagacg
     4201 atgcgctgga acctgatcca tacccacgcg gcgagcaatt gcagcccgag ggcgacgtac
     4261 gctgtcagga caatcagcca gaccaccatg gcctggtcgt tgccaaggcg acgccaacga
     4321 gagatcggaa actgctggac gaattgcagc aatcgcagca tgtcacacct gcttcttcgt
     4381 ttcgccaccg tcccagcctg gaagccctcg tccggcatca atgcgccgat tgctgccggc
     4441 cgcttccgcg ttcctcgcct ggcggagtcg cagacactcg gcgaaccagc gcagtgctag
     4501 ccggggcgcc gctctgatgt cagccgttcc cgagctgcgg aaagccccgc gatttgctcg
     4561 gccgtggcct gcggatagga caggttcagg ctctcaatcg cattgacgat gatgccgcca
     4621 acgaccgcgt gggtaatcca cttgtgatcg gcggggatca cgtaccacgg cgcccagggt
     4681 gtcgatgtct tcgtcaacgc gtcttcgaag gcgtccgtgt actggtccca gaattggcgc
     4741 tcggcgatat cgccggacga gaacttccag ttcttatccg ggttgtcgat gcgacgcagg
     4801 aagcggttgc gctgctcgtc ccacgagacg ttcaggaaga acttgagaat cttcgtcccg
     4861 ttccggtcga gatgccgctc gaacgcgttg atgtcttcga agcgctcttc ccacatgttg
     4921 gggccaactg cagacggcgg gtgcttctgg tgttgcagga tctccggatg gacccgaacg
     4981 accagcactt cctcgtaata cgagcggttg aagatgccga tccgcccgcg ttccggcaat
     5041 cccttggaat agcgccagag gtaggtgtga tccagatcct cggaggacgg cttcttgaac
     5101 gagaagacct ggcagccctg cgggttgacc ccggtcatca cgtgcttgat cgtgccgtcc
     5161 ttgccggccg catccagcgc ctggaagacg agcaggagcg cgtacgtgtc atcggcccac
     5221 aggcgcttct gcgcgtgagc gagctcctcg acgaactcat ccagccgctg tttggcgagt
     5281 ggcatcaact cttcgctgtc gagccccgga taatactcct caccacgcca tgccgcatct
     5341 cgcccgcgga tgttcgccgg cgtcccaggt tcgacccgca tctggcgaat gatctcgtcg
     5401 ctgacggcca tgctgtgtcc cttccagcgt attcgacgtg tcggtctcgc tcgtgtgtgg
     5461 aagtgctgcc ggatgctctc gaagctgacg gttgaagctc ccacgcaagt ccgcgagatc
     5521 agcaccgctc acgatcgggt gttacgtttt cgacgttttc aagaggttgg agtagtaatc
     5581 gagcgcagtc ttcgtcttgg tctgctggac actgcccgtt gagatccccc ggttgtggag
     5641 gaatgcgcgg acctcggcga caacctggaa cgtttcgttc ggcgcgcact tggtcgagag
     5701 ctcgagcaac cgctgaccgt tcggatagtt ccacagctcg gcgaccatct tcctggagaa
     5761 tcccttagga ttgaacttca gcttgaaaac cgtgatgggg ccgagcatag ccagcccatc
     5821 cagcgccaag cccgtcgggg cgtgcgttgt atagaacgcc cgctgctcct tcgagaacag
     5881 cttgcgcacc gggtgctgac cggccatcac ggctttgata ttgacatcat ccgcctggcc
     5941 cttgtatgac gccgagcaga cataccctcc cggcatcgcg tcgacctcaa caccgacgtt
     6001 gggcaggttg cgaatgtctg gcggaagctg gtccggcacg accggccgaa gcttgatgac
     6061 ggtgtcgccg cccttgacct ggacccgacg agcgcgaacg accacccccg cctgattcag
     6121 agccaggtcc ggagtgtcga agaacatgac ctggcgaatc tccgcgtcga gcggatcgac
     6181 ctcaagcgac tggagcgtcg tatagcgggc atcatcaggg atcgtgagct tgagctcgac
     6241 cgtcttcgcg cccttgatca gacccatcaa tgtcaccaga tcggcatcgc tcatgcgctg
     6301 acggccgttc tcggtaactg ctgttccttc ggtagccgtt gcggtcattg gttggtctcc
     6361 attcctacgt tgcgggttca agcacctgct cgccggacgt cgctccacat cccagcacga
     6421 acctcccgat gttggacagc gaccaacgtc gtgagcgatt cgagatcatc aacgtcgttg
     6481 gtcctactat gcagcgacct ctcgaccggg tcagcatcct tctggtgtag ttgcaggtcg
     6541 tagttcatcg cacagctcca ggtgaacaac gcctcccgtc gatggcccgc tgcggcctaa
     6601 gaaaagcagg ccagcagccc gttccgccgg cccgctactc accgtgcact ggatatcgcc
     6661 gtacgggtac cgtattggcc gcagatgtca ctggcgaggc cgccgggaca ggcgacggtc
     6721 ggtccgggct catgagcccg agatcgatca gctttctgat tcgccggatc gtgagtgggt
     6781 gtgaccggaa aatggtgacc aactctaccc agaccccgcg aacctgttgc tcctgatgta
     6841 gtacttcgct cacgttcgcg tagtggtacg cgtagcgccc gcaactcagc agaacgagtc
     6901 catcgacgcc gttgggtgac agccatgcgc catgccggtc ggaggtgtac tcccgcgcac
     6961 gcgagagaca atagctgatg accggcagca tgctgaagaa gagaatcgag agttgatacc
     7021 agatcttggt gtggttccgc ttgatgtgcc ccagctcgtg accgatgatg aatgccaacc
     7081 cctcgcgatt gttgtcgtac agattggcga acagctcatt gctgatgacg acgtaatccc
     7141 ggccaaccga cgagctggca aacgcgttga gcgtaccgtt cccgttcacg agatagatct
     7201 ctggatcgcg ccgcagcccg agccggcgtg cgtagtcgtc cttgacggca tagatgtccg
     7261 ggaactgatc tggtgagagc tgaaccgcct ggcccagcac gctggcgcgc tgtacctgtc
     7321 gcccaatgac gatcgccggt atcagcagca ggagcgcata catggcgtac tgaatggcat
     7381 cgctggtgac gccgtcgctg atggcaccgg gcaggtcctc cagccaccag tagttcgtga
     7441 cgattgcggc aacgatcacg acgttgacga ccacgcccag gatgtagagc ggcatttctg
     7501 cgggattgcc aagggatggc gacgttgatg ccatgcaagc cttccttctc gcatcaggac
     7561 cgtcgggctc ctcagtggct cagcggcttc atttcctccc gggtcagccc gacttctcgg
     7621 ccaatccaga tcaggaacgt gagcggcaat ccagccatga tcaccaggcc gatgaagacc
     7681 cagatgccgc caggcgggtc gtagtcaccg acctcggcgg ctatgtagct ggcaatgact
     7741 ccgaccacga ccagtacaag aggggtcacc catgggtgta ccggccgcag cgcgtaaccg
     7801 accacgaagg ctaccgccca gcagcatgca gccgcgatga acccggagac ggactcgtcg
     7861 aacagcagca cgaccgcggt ggtgatcgcg atcatgacaa ctatcggtag caggtacggt
     7921 cgcacccaat cgtgcgtgct ggtcttctcc acgacgcccc ctctttcctg ggctggcaat
     7981 cgaactcgtt gatgtcccgc aatgaggtga tcgtatggtg cgtggcggtc tgaacgtatc
     8041 agccagaggg gtgtagaaca gggtgcaact ccgcacgacg cgcggggcat tccaactgcc
     8101 ggaagatcgt ctgcgtcgcg actccgacgc cgtcaggaga gcgcggccta ctggaagatg
     8161 aacgggcagc cctcgccgag cgagctgaac gtgtagacac ctggagacac ctcgctgagc
     8221 tcggtctgcc agttgacgac cttggtctga gtgtcgccgc ccgtcgtcac atcgaccgtg
     8281 ccgctcatct tcatgaccgg cggctcgacg atgactgaga cgaagacgtt gctggtctcc
     8341 aagtctgccg gcttgcaggc ggccagctca tcaatgcggg actgcggcac gacgccggac
     8401 agggtcgcgg tcagcgcgtc cttgtcgccg ctcgcgaccg cgttcaccag gtcggtgccg
     8461 aattgctcga gctcagctgt cgtgtacaga ctgtcggcaa cgccttccgt gccggcggca
     8521 tcgctcgtcg tgccggtcgt cgccgttgtg ccagacgaat cggtcgcgtc gtcgtcgtcg
     8581 tcgtcacccc cgcagcccgt cagcgagacg gcgagaagga cgacgaccat catgagcatc
     8641 gcgtggcgga tcatcgcggc gcctccagtt cggaaccacc cggcgtttgt gaacggcagg
     8701 actggtcgat ggttcgcatc ttcggttcgt gagctttgga gatgtacgtc gcaggcaacg
     8761 accggtctgg agtacggacg aaggttggcc cagacacggt cgtcggccat ctggatcggc
     8821 tgaacatcaa tctgccgctg cgccctgccc gccgaatgga tccggcaggt cccatgagat
     8881 caggcggcgg gattccacct caagttgcgc ctgaagcgct tcaacggtcg cttctttcga
     8941 tgtcccggta ctgacgatga tccaggctgg atgattgagg gctacgacgg tgctgcggac
     9001 tgaaccgatc gcgaaccatt cgcctgctgg agtttgtccc agcgaataga cggccaccag
     9061 tccattgctg tggattgggg acgctaccac gagatccatg tcagtgtctg tcagtaccat
     9121 ggccctgcct tcaacaccct ctctacacgc gagcagactc gtacgccggt tgctggcggc
     9181 ccagcccggg cgcgtcggaa aggccgcgag ccgcatcgtg ggcgacgaac tcacccgcga
     9241 cggttgggcc agatcgatcg tctgtaaggg agactagctg gattcaccag ctgccagaac
     9301 gaccggatgg gtgcaaactc agggtgtacg cggcggtcaa cccttcgccg cttcgttgat
     9361 catcggagga gaatcgggct gacgtgcgtg cagcagtccc agctcacgtg cccggtgaac
     9421 cgcagccgtg cgacctgtca caccgagctt ttcgaacagg cgagagatgt cgagtttgaa
     9481 ggcgtcgacg gagacggaaa ggatgtcgct cagttcttcg acggtcagcc caaccgcgag
     9541 caaacggatg atgctcaact cccgcaacgt caacggcctg gcgggtccga gtcgccggtt
     9601 ccactggtct tcatcggcgt caaacggact cgcggccagg agctgccgca ctgcttcgac
     9661 ctgaacgccg cgcgcgtgg
//
LOCUS       DASUGJ010000631         8637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1771053, whole genome shotgun sequence.
ACCESSION   DASUGJ010000631 DASUGJ010000000
VERSION     DASUGJ010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771053"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2574
                     /locus_tag="VFV93_14475"
     CDS             <1..2574
                     /locus_tag="VFV93_14475"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX5166606.1"
                     /translation="GEDSSIYRSTDGGDSWTEITRNPGLPTGVLGKIGVAISPAKAGR
                     VWALIEADDGALFRSDDNGETWERVCDQGDLRRRAWYYIHIVADPSDENTVWVLNLKC
                     WKSIDGGKTFTAIPTPHGDNQDLWIDPTDSNRMIEGNDGGANVTFNGGRSWSSIYNQP
                     TAQFYHVTTDDRTPYTIFGSQQDNTALGGPSSSARGAITLNEWFQPGGGESGYIAIKP
                     TDDNIIYGGAIGSGAGNGRLLRFDRRTKQSSIVTVWPEVTGMGRGAEALKYRFQWTFP
                     ISFSPHEENTLYVTSNVVHRSTDDGMTWEVISPDLSHNDPETLKPSGGPITRDNTGAE
                     AYGTIFAFVESPHERGFFWAGTDDGRLHTSEGGKEWSEVSIPGLERALISIIEVSPHD
                     AATAYVAATRYKHDDFAPYLYKTTDYGKSWTRITNGIPENDFTRTIREDPNQKGLLYA
                     GTETGLYISFDDGENWQPFQSNLPVCPIHDVIVKGTDLIAATHGRSFWVLDDLTPLYQ
                     ISAGLASEPAAVLAPRTVKRFRHEGRPGDPGPSGFKSYGRFGGMMGTSYQRTNEYGEN
                     VITFLDAGKNPPDGAVIHYWLKDTPKGKVTLTIRDGKGNLIRRFESKTDTTQQAETEP
                     VTGEGYEGQTSGEDAPAESLKLPADAGMNRFVWNLRYANAKPAKGVDMAADALLGPIA
                     VPGKYTAELAVDGKTYSSEITIEADPRVGASAKDLQAQFDLLMQIRDKQSETHVAITT
                     IRTIKEQLDGWSARATDAGQKEQATALRDKLVAIEEVLVQPKASDPRQFPNGLNDKLA
                     ALPGMISNADTRPANQYTEVFKKLSGEVDEQLSKLKDVLSKDVKAFNDAVRSADVVAV
                     G"
     gene            2765..5989
                     /locus_tag="VFV93_14480"
     CDS             2765..5989
                     /locus_tag="VFV93_14480"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX5166607.1"
                     /translation="MSTPNGAGASLLQSLKWRSIGPHRGGRVVAVAGDVSNRETFYMG
                     ACAGGVWKTTDGGVIWRNVSDGFFKTAAVGAIAVSESDPNVIYAGMGETAIRGNVSHG
                     DGVYKSTDGGKSWQHMGLTETRHIGDIAIHPTNPDLVYVAALGHVWGPNEERGVYRSK
                     DGGQSWEKILYKSERAGSHELAMDPNNPRIIYAAIWDAQRYPHALRSGGDDSGIWKTT
                     DGGDTWTEITRNTGLPKGVLGKIGLAVSGARDNRVWALMEAHDGALFRSDDGGESWQR
                     LSEEPYLRTRAWYYMHIYADPKDADTVWVLNYGIHKSIDGGKTFEAVPSRHGDEHDLW
                     IDPNDTQRMIKGDDGGACVTYNGGRSWSTIFNQPTAQLYHVTTDDNLPYRVYGSQQDN
                     SAISVPSATVEGAIHERDWFEPGGGESGYIALKPGDANIMVAGAIGSGNFNGRLIHYN
                     RTTGQHRNITVWPELAGMGSGAEALKYRFQWTFPIFWSQHEQDALYVAGNRIFKSLDE
                     GKSWEVVSEDLTRNDPETLKPSGGPITKDNTGAEAYGTIFALEESPHQRGLLWAGTDD
                     GLVKISEDGGKNWQNITPAELPEWALISIIDLSKHDAGSAYMAATRYKSHDQKPYLFK
                     TKDFGTNWTLIANGIPEDDFTRTIREDPEQPGLLFCGTETGLYVSYDDGENWQRMGGN
                     FPVVPVHDLVIKGNNLVIATHGRSFWILDDLTPLRQMAAGTLATDNALVLFPAAPKVR
                     MKVYPGFGSDPAKGYVHYRGVGTSNIAMEVIEKPDGTSETHLLDAGENPPDGLVLQYY
                     LREKPKEPITLTIRDADGNEVRTFSSKAEKTPPAGAEAEGAEGAESGSAADEEPKGDT
                     FVPANAGVNRFDWDMRHAPAETAEGMDLKPWEKPLGPVVLPGTYTVEIKAGDATASQQ
                     VEVVKDPRVDVSDADLKAQFDMLLQIRDRLADTTKAIGRVRAMRSQLESWEKRTKDSD
                     RGEQIAAAGKAARDELTAIETELIDTTTKSPLMSPSRLFEKLNALTEFVDSADVAPAK
                     QSVEVFEKLSGELDDQLARIDAAVENQIGAFNKLIQEAELQPVG"
     gene            6103..6936
                     /locus_tag="VFV93_14485"
     CDS             6103..6936
                     /locus_tag="VFV93_14485"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5166608.1"
                     /translation="MSDRSLLAVFAHPDDESICAGTLARYAAGGCRVVLVCATRGEVG
                     EISDPALATPETLPAVREGELRCATRAMGIGEPIFLGYRDSGMDGTDDNRHPDAFVNA
                     DPAEVVSRLVRIIREVRPQVVITFDETGGYGHPDHIAVWRHTHAAVEAAATCDDDPDA
                     GEPWQVERIAYAVFAKRFFTELRELLVSHGEDTSELDEFAELGLGFPDDRIHAVVDVS
                     AFLDQKAAAFECHRTQFGGDTFFSRVSEDETRQMRSREYFSFGWPAGIAGQMEDDLFP
                     A"
     gene            7012..7845
                     /locus_tag="VFV93_14490"
     CDS             7012..7845
                     /locus_tag="VFV93_14490"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5166609.1"
                     /translation="MRDAGWQNDLYGQLAHAAAAQVNPLAAGLLETLDDPRFKSMRDT
                     FLEDLHLYPGSATLEVGCGPAMLLEAIHERTGPDGIIVGLDINPHFIAIANQRAAMLG
                     IDRARFVTADCHTLPFDDQSFDSIVAEKLLMHVAPIRRVIGEMARVLSIGGRLVLVDY
                     DPYTAFAAGPNPTITSRVMASAATVYASPQAARETPSACVDVGLYVERVHGHLLVFED
                     PQARTARGIAPVWADHAVMGRQADPGTIQRWLKAVERAVEQRQFLISIPHVITVATRI
                     S"
     gene            7930..>8637
                     /locus_tag="VFV93_14495"
     CDS             7930..>8637
                     /locus_tag="VFV93_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5166610.1"
                     /translation="MSEDYAGFARPEMIVAPEWLAQHLDDPSLRIIDCDQAEVAMTRA
                     HVPGAVMLPIHSYFRNTETGVGVATAEQTETIMRDLGVSRNTRVICYDSTGGTLAARI
                     WWVLWYYGHEQVAVLDGGWTAWQAAGLPTETSWNKPAAGDWTAEQHDERIAGCDVMLP
                     WVGQADFVPLDVRAYSEWSGTQPNPANQREGHIPGAVHIEWRDFVNWDNHAKLKPAGE
                     IRAMLEAAGVTRDKRVVP"
BASE COUNT         1667 a         2798 c         2835 g         1337 t
ORIGIN      
        1 ggcgaagatt cgagcatcta ccgctcgacc gacggtggcg atagctggac ggagatcacc
       61 cgcaatccgg gcctgccaac cggcgtgctc ggcaagatcg gcgttgccat ttctccggcc
      121 aaggccggtc gcgtctgggc cctgatcgag gccgacgacg gcgcgctgtt ccgctcggac
      181 gacaatggcg agacatggga gcgcgtctgc gatcagggcg atctgcggcg ccgcgcctgg
      241 tactacatcc acatcgtcgc cgatcccagc gacgagaata ccgtctgggt gctgaacctg
      301 aagtgctgga agtcgatcga cggcggcaag accttcacgg cgatcccgac gccgcacggt
      361 gacaatcagg atctctggat cgacccgacc gactcgaacc ggatgatcga aggcaacgac
      421 ggtggcgcga acgtcacctt caatggtggc cggagctggt cgagcatcta caaccagccg
      481 acggcgcagt tctatcatgt gaccaccgac gaccggacgc cgtacacgat cttcggctcg
      541 cagcaggaca acaccgccct cggcggtccg tcgtcctcgg cgcgtggcgc gatcaccctc
      601 aacgagtggt tccagccggg aggcggcgaa tcgggctaca tcgcgatcaa gccgaccgac
      661 gacaacatca tctacggtgg tgcgatcggc tccggcgccg gcaatggccg gttgctgcgc
      721 ttcgaccggc gtacgaagca gtcaagtatc gtcaccgtct ggccggaagt aacgggaatg
      781 ggacgcggcg cggaggcgct caagtaccgc ttccagtgga ccttcccgat cagcttctcg
      841 ccgcacgagg agaatacgct ctacgtgacc tcgaacgtcg tccaccgctc gaccgacgac
      901 ggtatgacct gggaagtgat ctcgccagac ctctcgcaca acgatcccga gacactcaag
      961 ccctccggcg ggccgatcac gcgcgacaac accggcgccg aggcgtacgg gacgatcttc
     1021 gccttcgtcg agtcgcccca cgagcgtggc ttcttctggg ccggcaccga tgacggccgg
     1081 ctgcacacct ccgaaggcgg caaggagtgg tccgaggtca gcatccccgg tcttgaacgg
     1141 gcgctgatct cgatcatcga ggtctcgccg cacgacgccg cgaccgcgta cgtcgctgct
     1201 acccgctaca agcacgacga cttcgcgccg tatctgtaca agacgacgga ctacggcaag
     1261 tcctggacgc ggatcactaa cgggatcccg gagaacgact tcacccgaac gatccgcgaa
     1321 gatccgaacc agaaggggct gctgtacgcc ggaaccgaga cgggcctcta catctcgttc
     1381 gacgacggcg agaactggca gccgttccag agcaacctgc cggtttgccc gatccacgat
     1441 gtgatcgtga aaggcaccga cctgatcgcg gcgactcacg gccgctcgtt ctgggtgctg
     1501 gacgacctga cgccgctgta tcagatcagt gccggactgg cgagcgaacc ggcggccgta
     1561 ctcgcgccgc gaaccgtcaa gcgtttccgg catgagggcc ggccgggcga tccggggccg
     1621 agtggcttca agtcctacgg ccgcttcggc gggatgatgg gcaccagcta ccagaggacg
     1681 aacgagtacg gagagaacgt catcaccttc ctcgacgcgg gcaagaaccc gcccgacggg
     1741 gccgtcatcc actactggct caaggacacg ccgaaaggca aggtgacgct gacgatccgc
     1801 gacggcaagg gcaacctcat ccgccgcttc gagagcaaga ccgacacgac ccagcaggcg
     1861 gagactgagc cggtcaccgg cgagggctac gaagggcaga ccagcggcga ggacgcgccg
     1921 gccgagagct tgaagctgcc ggccgacgcc ggcatgaacc gtttcgtctg gaacctgcgc
     1981 tacgccaacg ccaagccggc caagggcgtc gacatggccg ccgacgcgct gcttggccca
     2041 atcgcggtgc ctggcaagta cacggccgag ttggccgttg acggcaagac gtacagcagc
     2101 gagatcacga tcgaggccga tccgcgtgtc ggagccagtg cgaaggatct ccaggcacag
     2161 ttcgacctgc tgatgcagat ccgtgacaag caatccgaga cgcacgtggc catcacgacg
     2221 attcgcacga tcaaggagca gctcgacggc tggagcgcga gagcgactga cgcgggccag
     2281 aaggagcagg cgacggcgct gcgcgacaag ctggtcgcga tcgaggaggt gctggtccaa
     2341 ccgaaggcga gcgacccgcg ccagttcccg aacggcctca acgacaagct ggccgccctg
     2401 cccggcatga tctccaacgc cgacacacgg ccggccaacc agtacaccga ggtcttcaag
     2461 aagctctccg gcgaggtcga cgagcagctc agcaagctga aggatgtcct cagcaaggac
     2521 gtcaaggcgt tcaacgacgc tgtccgaagc gcggatgtgg tggcggtggg ataaaagtcc
     2581 acgtctccgc ttggcgacac aattgagttg ctaatgcaat ccggtagggg cgatatcgca
     2641 atatcgcccg gcgccccgcc cgacgcgctc gggtgatacc agggcgatgt tgcgatatcg
     2701 cccctaccgg attgcattgt ttagattgca gcgcaggtct gccataatgc aagaaaggac
     2761 gaccatgtcc actccaaatg gagccggtgc aagcctcctc cagtccctca agtggcgctc
     2821 gatcgggccg catcgtggtg gacgggttgt cgccgtcgcc ggcgatgtgt ccaaccgcga
     2881 aacgttctac atgggcgcct gcgctggtgg cgtctggaag acgaccgatg gcggcgtgat
     2941 ttggcgcaac gtctcggatg gcttcttcaa gacggccgct gtcggggcga tcgccgtctc
     3001 ggagtctgac ccgaacgtga tctacgccgg gatgggcgag actgccatcc gtggcaacgt
     3061 ctcgcacggc gacggcgtct acaaatcgac cgatggcggc aagtcctggc agcacatggg
     3121 gctgaccgag acgcgccaca tcggcgacat cgcgatccac ccgaccaatc cggatctggt
     3181 ctacgtcgct gccctcggcc acgtctgggg gccgaacgag gagcgcggcg tctaccgcag
     3241 caaggacggc ggccagagct gggagaagat cctctacaag tcggaacggg ccggcagcca
     3301 cgagctggcg atggacccga acaacccgcg tatcatctac gctgcgatct gggacgccca
     3361 gcgctacccg cacgcgctcc gctccggcgg tgacgactcc ggcatctgga agacgaccga
     3421 cggcggcgac acctggaccg agatcacgcg caacacaggg ctgccgaagg gcgtgctcgg
     3481 caagatcggc ctggccgtct ccggcgctcg cgacaaccgc gtctgggcac tgatggaggc
     3541 gcacgacggc gcactcttcc gctccgacga tggtggcgag agctggcagc ggctgagtga
     3601 ggaaccgtac ctgcgcaccc gcgcctggta ctacatgcac atctacgccg acccgaagga
     3661 cgccgacacg gtctgggtgc tgaactacgg catccacaag tcgatcgacg gcggcaagac
     3721 ctttgaggcg gttccgtcgc ggcatggcga cgagcacgac ctctggatag acccgaacga
     3781 cactcagcgg atgatcaagg gtgacgacgg cggcgcctgc gtcacctaca acggcggccg
     3841 atcgtggtcg accatcttca accagccgac ggcccagctc taccacgtca caaccgacga
     3901 taacctgccc taccgggtct acggctccca gcaggacaac tcggccatca gcgtcccgag
     3961 cgcgaccgtt gagggtgcga tccacgagcg cgactggttc gagccgggtg gcggcgagtc
     4021 gggttacatc gccctcaagc cgggcgacgc caacatcatg gtcgccggtg cgatcggctc
     4081 cggcaacttc aacggccggc tgatccacta caaccggacg accggtcagc accgtaacat
     4141 caccgtctgg ccggaactgg ccgggatggg ctcgggggcc gaggcgctca agtaccgctt
     4201 ccagtggacc ttcccgatct tctggtcgca gcacgagcag gacgccctct acgtcgccgg
     4261 caaccgcatt ttcaagtcgc tcgacgaggg caagagctgg gaagtcgtct ccgaggacct
     4321 gacccgcaac gacccggaga cgctcaagcc gtccggcggg ccgatcacga aggacaacac
     4381 cggcgccgag gcgtacggga cgatcttcgc gctggaagag tcgccgcacc agcgcgggct
     4441 gctctgggcc gggaccgacg acggtctggt caagatctcc gaggacggcg gcaagaactg
     4501 gcagaacatc acgccggccg agctgcccga gtgggcgctg atctcgatca tcgacctctc
     4561 gaagcatgac gcgggcagcg cctacatggc ggcgacgcgt tacaagagtc acgatcagaa
     4621 gccgtacctg ttcaagacga aggacttcgg caccaactgg acactgatcg ccaacggcat
     4681 cccggaagac gacttcaccc ggacgatccg cgaggacccg gaacagccgg ggctgctttt
     4741 ctgcggcacc gagactggac tctacgtctc gtacgacgac ggtgagaact ggcagcggat
     4801 gggcggcaac ttcccggtcg tgccggtcca cgacctggtc atcaagggca acaacctggt
     4861 catcgccact cacggccgtt cgttctggat cctcgacgat ctgactccac tgcgccagat
     4921 ggcggccggt acgctggcga ccgacaacgc gctggtactc ttcccggcag cgcccaaggt
     4981 tcggatgaag gtctatcccg gcttcggtag cgaccccgca aagggatacg ttcactaccg
     5041 gggcgtcgga accagcaaca tcgcgatgga ggtgatcgag aagccagacg gtacctcgga
     5101 gacacacttg ctcgacgctg gtgagaatcc gccggatggg ctcgtcctcc agtactatct
     5161 gcgtgagaag ccgaaagagc cgatcacact cacgatccgc gacgccgacg gcaacgaggt
     5221 acggacgttc tccagcaagg cggagaagac tccaccggca ggcgccgaag cggagggcgc
     5281 cgagggcgct gagagcggca gtgcggccga cgaggagccg aagggtgaca ccttcgttcc
     5341 ggcaaatgcc ggcgtcaacc gcttcgactg ggacatgcgg catgctccgg ccgagacggc
     5401 cgaggggatg gacctgaagc cgtgggagaa gccgctcggc cccgtcgtcc tgccgggaac
     5461 ctacacggtt gagatcaagg ccggcgacgc gaccgccagc cagcaggtcg aggtcgtcaa
     5521 ggacccgcgg gtcgatgtca gcgacgccga tctgaaggcc cagtttgaca tgctgctcca
     5581 gatccgtgac cggctggccg acacaaccaa ggctatcggc cgtgtccggg caatgcgctc
     5641 gcaactggaa agctgggaga agcgaaccaa ggacagcgac cggggcgagc agatcgccgc
     5701 agccggcaag gctgcccgcg acgaactgac cgcgatcgaa accgagctga tcgacacgac
     5761 gaccaagagc ccgctgatgt cgcccagccg gctcttcgag aagctcaacg cgctgaccga
     5821 gttcgtcgat agcgccgacg ttgcgccagc caagcagtcc gtcgaggtct tcgagaagct
     5881 ctccggtgag ctcgacgacc agctggcccg catcgacgcc gccgtcgaaa accagatcgg
     5941 cgccttcaac aagctgatcc aggaggccga actccagcca gtcggctaac gacggcaccc
     6001 tctacacaac aagtcggggc cggcacgagg ccggccccga cttgtgttct gactggtatc
     6061 ctcagttgct gccttggtga tacgtcttga gaggatgtca tcttgtccga tcggtctctg
     6121 ctcgcggtgt tcgcccatcc ggatgacgaa tcgatctgcg ccgggacgtt ggcgcgctac
     6181 gctgctggtg gttgccgggt cgtgctggtc tgcgcgacgc gcggcgaggt gggcgagatc
     6241 agcgaccccg cactggcgac gccggagacg ttgccggcag tgcgggaggg cgagctgcgc
     6301 tgtgcgacgc gggcgatggg catcggggag ccgatcttcc tcggctaccg cgactccggc
     6361 atggacggga cggacgacaa ccgacatccg gacgccttcg tgaacgccga cccggccgag
     6421 gtcgtcagcc ggctggtcag gatcatccgc gaggtccgcc cgcaggtcgt gatcacgttc
     6481 gatgagacgg gcggttacgg gcatccggat cacatcgcgg tctggcggca tacccacgct
     6541 gccgtcgagg cggctgccac gtgcgatgac gatcctgacg cgggcgagcc gtggcaggtc
     6601 gaacggatcg cctacgcggt cttcgcgaag cggttcttca cagagttgcg cgagttgctg
     6661 gtgagccacg gcgaagacac cagcgagctg gatgagttcg ctgagctggg gctgggcttc
     6721 ccggatgatc ggatccacgc ggtcgttgat gtctcagcct tcctcgatca gaaggcggcc
     6781 gccttcgaat gtcacaggac acagttcggt ggcgatacct tcttctcccg tgtcagcgag
     6841 gacgagacgc ggcaaatgcg cagccgtgag tacttctctt tcggctggcc agccgggatc
     6901 gctggtcaaa tggaagacga tctcttcccg gcatgacggg ttggcgaatc gcgcatgctc
     6961 tgttagcgtt gccggaacgt cgtcgaggca ccgagcgctg ggacatagtt cgtgcgtgat
     7021 gctggctggc aaaacgatct ctatgggcaa ctggcgcacg ctgccgccgc ccaggtcaac
     7081 ccgcttgcag ccggccttct cgaaacgttg gacgatcccc gtttcaagtc catgcgggac
     7141 acatttctcg aggatctcca cctctaccct ggttcggcga cgctggaagt tggctgcggg
     7201 ccggcgatgc tgctggaagc catccacgag cggaccgggc cggacggcat catcgtcggg
     7261 ctggatatca acccgcactt catcgcgatc gccaaccagc gagcggccat gctcggcatc
     7321 gatcgggccc gcttcgtcac tgccgactgc cacacgctgc cgtttgacga ccagtcgttt
     7381 gattcgatcg tcgcggagaa gctgctgatg cacgtcgcgc cgatccggcg agtgatcggc
     7441 gagatggcgc gcgtcctctc aatcggcggc cggctggtgc tggttgacta tgatccgtac
     7501 acggcgtttg ccgccggccc gaatccgacg atcacgtcac gggtgatggc ctcggcggcg
     7561 accgtctacg cgtcgcccca ggcggcacga gagactccaa gcgcctgtgt cgatgtcggg
     7621 ctgtatgtcg agcgggtcca cggccacctg ctcgtcttcg aggacccgca ggcgcgcacc
     7681 gcccgcggca tcgcgccggt ctgggccgac cacgccgtca tgggccgaca ggccgacccc
     7741 ggcacaatcc agcgctggct caaggccgtc gagcgtgcgg tagaacagcg ccagttcctg
     7801 atctcgatcc cgcatgtgat taccgtcgcg acccgcatca gttgagattg acaatttcgg
     7861 cacttcgcgg atgatggcgg cgcggccatg aacgggtcgc ctgtccaagt ctggagagga
     7921 cggtcgccga tgagcgaaga ctacgcgggc ttcgcacgcc cggagatgat cgttgcgccg
     7981 gagtggctgg cacaacacct cgacgatccg tcgttgcgga tcatcgactg cgatcaggcg
     8041 gaggtggcga tgacgcgcgc ccacgttcct ggcgccgtca tgctgccgat ccactcgtac
     8101 ttccgcaaca cggagacggg tgtcggcgtc gcgaccgccg agcagaccga gacgatcatg
     8161 cgggatctcg gcgtctcgcg gaacacgcgg gtgatctgtt acgactcgac cggcggcacg
     8221 ctggcggcgc ggatctggtg ggtgctctgg tactacggcc acgagcaggt cgccgttctc
     8281 gatggtggct ggacagcctg gcaggcggcg gggttgccga ccgaaacgag ttggaacaag
     8341 ccggcagccg gtgactggac ggccgagcag cacgacgagc ggatcgcagg ctgtgacgtg
     8401 atgctgccgt gggtgggcca ggccgatttc gtaccgctgg acgtgcgcgc ctacagcgag
     8461 tggtccggga cccaacccaa tccggccaac cagcgcgagg gccacattcc cggcgccgtt
     8521 cacatcgagt ggcgcgattt cgtcaactgg gataaccacg cgaagctcaa gccggccggc
     8581 gagattcggg cgatgctgga agcagccggc gtgacgcgcg acaaacgggt ggtcccc
//
LOCUS       DASUGJ010000632         4305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2049483, whole genome shotgun sequence.
ACCESSION   DASUGJ010000632 DASUGJ010000000
VERSION     DASUGJ010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049483"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFV93_14500"
     CDS             complement(<1..573)
                     /locus_tag="VFV93_14500"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5166611.1"
                     /translation="MVSSQTTTSATPAIDTAAIDAFAATLRGQLIRPGDAGYEIARRV
                     HNGMIDKHPALIARCVDVADVIAGVNFAREHDLLLAIRGGGHNGPGFGTCDDGLVLDL
                     SPLKGIRVDPVARTARVEGGCTLGDVDHATHAFGLATPLGIASTTGVAGLTLGGGMGH
                     LARHYGLAIDNLLEVDIVLADGSFVTASEQQ"
     gene            complement(714..3737)
                     /locus_tag="VFV93_14505"
     CDS             complement(714..3737)
                     /locus_tag="VFV93_14505"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5166612.1"
                     /translation="MAKPFNRTIICPVVIGRDTQLSLLARAFEEVAGAQGQTLLLAGE
                     AGIGKSRLVSEAKWIALRGGWRIVEGHCFETDRILPYAPLLDLLRTLLVGLRSEEIEA
                     LLGPEARDLIKILPELGRKLPGITTAPPLDPAQERRLAIQALTAVFLRLAGNGPALVV
                     VEDLHWSDDTSLDALLHLARDIRHSPILLLLTYRDDEITPELSHALATFDRERLADEL
                     PLRRLNVADVGTMLHAIFAGVSPVRGETLDTLHALTDGNPFFIEEILGTLVRAEESTE
                     PSDLQDRLALDALEEPQIPRSVQDAVQRRVERLTPEVRRVLELAAVAGRRFDFSLLQL
                     LTGYDETELLGLVKELIAAQLLVEEVEDRFAFRHALTREAVYSQLLSRERRRLHQTIA
                     KALEELNAVAEELPLADLAYHFYAAGEWQPALDYASRAGDQALALHAPRAAIEQFSRA
                     LDAAKRLSRSPASSLYRALGRAYETLGDFERARAEYEVALAAAREAHDQREEWRVLLD
                     LGLFWSGHDYERTAEYCRQALSLARTIGDPVILAHSLNRFGNWHTNVGRPRDALAYHE
                     EALAIFKTTDDRHALVETLDLLGIAAFIAGKPCDAARNYREAFDIFEELEATPQFYTC
                     LSTFSLCGSNYQTEGGATAPIVVDECIRLSERAIALAEEAGLRLGEAITQQIAGIVRT
                     AQGRYDRALEHMQRGLAIAEEIGHRQWTVSGQCEMGVIYLDLLDARRARHYLERARAG
                     AEAMGSWQWVLTISQQLAAACLLDRDLNAAQAVLDAAGLTGLPEQVGGARECWIYLAE
                     LHLARGNARETLGIADQLIATDPDITGERAVPRVARLRGEALMALGRLDEAEISLRAS
                     VDGAVALGRPGLVWRAQVALGNVLRRQRRHSEADASYAAARDVIRELAATVPESALRE
                     TFITNALAQMPRPRQPTPRRAARRTGSVKSNDRRTVDRQRADGRYPCQPRPQQARLQL
                     PHASGDLGCRSGAAERRVSLSHANSSTRQQKDHQT"
     gene            complement(3931..>4305)
                     /locus_tag="VFV93_14510"
     CDS             complement(3931..>4305)
                     /locus_tag="VFV93_14510"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5166613.1"
                     /translation="VDLSRNVAIAYINDVPVNAVPVITGKAGFETPVGVHTIFARVES
                     ETLDSATVGIPKGDPEYYYLPDVPYTQYFATGGFAIHSNYWSDDWQFGTASSHGCVNM
                     LPEDAAWFWWFLDIGSVVNVHY"
BASE COUNT          772 a         1422 c         1358 g          753 t
ORIGIN      
        1 ttgctgctcg ctggcggtca cgaagctgcc gtcggccagc acgatgtcga cttccagcag
       61 gttgtcgatc gccagcccat agtgacgagc gagatgaccc atcccgccgc ccagcgtgag
      121 ccccgcgaca ccggtcgtcg aggcgatccc gagcggggtt gccagtccga acgcgtgggt
      181 tgcgtggtcc acgtcgccga gcgtgcagcc tccctcgacg cgggccgttc gggcgactgg
      241 atcgacccgg atccccttca gtggcgagag gtcgagcacc agcccgtcgt cgcaggtgcc
      301 gaagccgggc ccgttgtgtc caccaccacg gatcgccagc agcagatcat gctcgcgcgc
      361 gaagttgaca ccggcgatga catcagcgac atccacacaa cgggcgatca gtgccggatg
      421 cttgtcgatc atgccgttgt ggacccggcg ggcgatctcg taaccggcat cgcccggccg
      481 gataagctgg ccacgcaacg tcgcggcgaa ggcgtcgatc gccgccgtgt cgatcgcggg
      541 cgtcgcgctg gtcgtcgtct gactggagac catctgcaca ccaacctctc tccagcgccg
      601 aacgcgccgg aattcgggtc gcgcggaccc agcctaccat cgggcgggat ccgcgcaatc
      661 actccttgca tcacgtgtct tcacagtaca gccgaagcga aagcggcaca tcatcaggtc
      721 tgatgatctt tttgctgtct tgtcgatgag ttcgcgtgcg aaaggctcac acgtcgttca
      781 gcagccccac ttcgacagcc caggtcgcca cttgcgtgcg ggagctgtag ccgagcttgc
      841 tgaggacgtg gctgacatgg gtagcgaccg tcggctcgct gacgatcaac cgttcggcga
      901 tcgttcgatt tgacagaccc tgtgcgacga gcggcgcggc gtggcgtggg ctgccgcggt
      961 ctcggcatct gcgccagcgc gttggtgatg aacgtctctc gcagggctga ttcagggacg
     1021 gttgcggcca gctccctgat gacatcccgc gcggcagcgt agctggcatc ggcctcgcta
     1081 tggcgccgct gccgccgcag aacgtttccc agtgccacct gagcgcgcca caccagccca
     1141 ggccgtccca gcgcgacggc gccatccacg gacgcacgga gggagatctc ggcttcgtcc
     1201 aaccgaccga gtgccatcaa cgcctcgccg cgcagccgcg ctacacgggg taccgcacgc
     1261 tcgccggtga tgtcgggatc ggtagcgatc aactggtcgg caatgcccag cgtttcgcgc
     1321 gcattgcctc gtgccagatg gagctccgcg aggtagatcc agcattcgcg cgctccgcct
     1381 acttgctccg gcaggccggt gagtcctgcc gcgtcgagga cggcctgggc cgcgttcaga
     1441 tcgcgatcca gcaggcaggc cgccgcgagc tgctggctga tcgtcaacac ccactgccac
     1501 gatcccatcg cctcggcacc cgcccgcgct cgttcgagat agtgtcttgc ccgacgcgcg
     1561 tccaacaggt ccagataaat cacgcccatc tcacactgcc ccgagacggt ccattgacga
     1621 tggccgatct cttccgcgat cgccagccca cgctgcatat gttcgagcgc tcgatcgtac
     1681 cgtccctgtg ccgtcctcac aattccagcg atctgctggg tgatcgcctc gcccagccgt
     1741 agccccgcct cctctgcgag cgcaatcgcg cgctcagaga gtcggatgca ttcgtcgaca
     1801 acgatcggcg cggtcgctcc accctcagtc tgatagttcg acccacacaa cgagaacgtc
     1861 gagaggcatg tatagaactg aggtgtggcc tcgagttcct cgaatatatc gaaggcttcc
     1921 cggtagttcc gcgcggcgtc gcacggcttg ccagcaatga atgccgcgat gcccagcaga
     1981 tcgagcgtct cgaccagtgc atggcgatca tcagtcgtct tgaagatcgc cagcgcttcc
     2041 tcgtggtaag cgagcgcatc gcgcggacgt ccgacattcg tgtgccagtt gccgaaccgg
     2101 ttcaggctgt gcgccagaat gactggatcg ccgatcgtgc gcgccagcga gagcgcctgc
     2161 cggcagtact cggcggttcg ttcgtagtca tggcccgacc agaacaggcc gaggtcgagc
     2221 aacacgcgcc attcctcgcg ctggtcgtgt gcctcacgtg ccgccgcgag cgcaacttcg
     2281 tactccgcac gggcgcgctc aaaatcgccg agcgtctcgt acgcgcggcc cagggcgcga
     2341 tagagcgacg acgcgggaga tcgcgacaac cgtttggccg cgtcgagcgc ccgcgagaac
     2401 tgctcgatcg cggcgcgcgg cgcatgaagc gccagcgcct ggtctcccgc tcgcgacgca
     2461 taatcgagtg ccggctgcca ctcgccggcc gcgtagaagt ggtaggccag atcggcaagc
     2521 gggagttcct cagcgacggc gttcagctcc tccagcgcct tcgcgatcgt ctggtgcagc
     2581 cgacgacgct cgcgtgaaag gagttgcgaa tagaccgcct cgcgggtcaa tgcgtgccga
     2641 aaggcgaagc gatcctcgac ctcctcaacc agcaattgcg ccgcgatcaa ttccttgacg
     2701 agacccagca gttccgtctc gtcataccct gtcagcaact ggaggagcga gaagtcgaat
     2761 cgccgtcccg cgacggccgc aagctccagc actcgtcgca cttcaggagt caaccgctcc
     2821 acccggcgct ggactgcgtc ctggacgctg cgcggtatct gcggctcttc cagtgcgtcc
     2881 agcgccagcc gatcctgcag atccgatggt tccgtcgatt cctcagcgcg tacgagcgtg
     2941 ccaaggatct cctcgatgaa gaagggattg ccatcggtca gggcatgcaa cgtgtcgagc
     3001 gtctccccgc gaaccgggga tacaccagca aagatcgcgt gcagcatcgt gccgacgtcg
     3061 gcaacgttga gtcgtcgcag gggcagttca tccgccaggc gctcgcgatc gaacgtcgca
     3121 agcgcgtgac tcagctcggg cgtgatctcg tcgtcgcgat acgtgaggag gagcagaatt
     3181 ggcgaatgcc tgatgtcccg agccaggtgt agcagcgcat caaggctggt gtcgtcgctc
     3241 cagtgcaggt cttcaacgac cacgagtgcc ggcccgttgc ccgccaagcg taggaatacc
     3301 gcggtcaacg cctggatcgc cagtcgacgc tcctgcgccg gatcgagtgg cggtgctgtt
     3361 gtaatgccgg gtagcttccg acccaactcg ggcagaatct tgatcagatc acgcgcttct
     3421 ggaccgagca gcgcctcgat ttcctcggaa cgcaagccca cgagcaatgt gcgcagcaga
     3481 tcgagcagtg gcgcatacgg gaggatgcgg tctgtctcga aacaatgccc ctcaacgatc
     3541 cgccagccgc cgcgcagcgc gatccatttc gcttcggata ccaggcgcga cttgccgatt
     3601 ccggcctcgc ctgcgagcag aagcgtctgt ccctgagcac ccgctacttc ctcgaaggcg
     3661 cgcgccagca gcgaaagctg cgtgtcacgc ccgatgacga cgggacaaat gatcgttcga
     3721 ttgaatggtt tcgccatccg agatgcctct caggcggcca gatgcggcgc atcatcgcgg
     3781 tcggctgcgc tattgtctgt gtgtaaccgt agtataacgc caactcagac atgcggggcg
     3841 gccgcgagag tcgccatggt gaattggaag tggctcgcga tggggccatt cgccagcaac
     3901 caggaggagc gtcctggttg cagacacgcc ctagtaatgg acgttgacga ccgagccgat
     3961 gtcgaggaac caccagaacc aggcggcgtc ctcggggagc atgttgacgc agccgtggct
     4021 ggaggcggtg ccgaactgcc agtcgtcgga ccagtagttc gagtggatcg cgaagccgcc
     4081 cgtcgcgaag tactgcgtgt acggcacgtc gggcaggtag tagtactccg ggtcgccctt
     4141 cgggatgccg accgtcgcgc tatcgagtgt ctcgctctcg acacgggcga agatggtgtg
     4201 gacgccaacc ggcgtctcga accccgcctt gccagtgatg acgggaacgg cgttgaccgg
     4261 aacatcgttg atgtaggcga tggcaacgtt gcgggacaga tcgac
//
LOCUS       DASUGJ010000633         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_521676, whole genome shotgun sequence.
ACCESSION   DASUGJ010000633 DASUGJ010000000
VERSION     DASUGJ010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521676"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VFV93_14515"
     CDS             <1..1585
                     /locus_tag="VFV93_14515"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5166614.1"
                     /translation="SVQRIELASQVEVASRAILAISSELSLDMVLNRIVDLAREISGA
                     RYAALGVPGPSGELESFLTSGISSEEVAAIAHRPEGRGLLGLLLREPGTVRLANLAEH
                     PVSVGFPANHPPMRSFLGVPIMAHGKVLGNLYLTEKRTGSAFTDDDARLVEELARHAA
                     VAIENAHLYQQVGQQQQRLQLILDQLPEATLLAERDPERVTLANQHASNLLGWQIETP
                     IPLETFLQRNPRLGQDGTPMPAESIPMVESLRTGERISHREVRIGRADGQVMTALVNS
                     VPLRGPDGEITGAIAVFQDITQIKDAEQLKDDFLSLVSHELRTPLTTIQGGALMLQRD
                     WAVLDDETRQDLLADIANETRRLGGLIENMVQLANIRAGRMRMETEPVHVLRLLDGAI
                     AATRQLDPERVVTLAADADLFAEADPDRLDQVIRNLLSNAIKYAPPGLPVEVTANEQD
                     GMVTITVRDYGPGIADTDLPRIFDRFSRAGRAVSSGAPGMGLGLYLVRHVVEAHGGSI
                     WIERPDDGGTRVVLTIPAS"
     gene            1695..2474
                     /locus_tag="VFV93_14520"
     CDS             1695..2474
                     /locus_tag="VFV93_14520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5166615.1"
                     /translation="MKTPQDLFDLTGKIAIVTGAGSGLGVVFAQALAEAGAGVVCADL
                     RLDGAEETARSIEELGRPALAVETDVTDEAAVQSMVQTTLDRFGRIDILVNNAGIGVG
                     GSPEETTLADWQAAVNVNLTGVFICAREVGKAMIAAGTGGRIINIASILGAVASEPAP
                     VTAYAATKGAVINLTRDLAVHWAPHGILVNALGPAYFPSAMTGDALAMPEVMAEIERR
                     TPLGRVGRLEELKGPVVLLASDAGSYITGQTLFVDGGWTAW"
     gene            2588..>2887
                     /locus_tag="VFV93_14525"
     CDS             2588..>2887
                     /locus_tag="VFV93_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166616.1"
                     /translation="MESETTAHRPRLHPLHARPRSMSLPRPGPLVLPRLTLLLVLALL
                     VWAGMQQLSTPAVVPASAPATEFSAARAMQHLRVIASAPRAVGLAGHTATREYLVA"
BASE COUNT          457 a          972 c          997 g          461 t
ORIGIN      
        1 cagtgtgcag cggatcgagc tggccagcca ggtcgaggtc gccagccgcg cgattctggc
       61 gatcagcagc gaactatcgc ttgacatggt cctgaaccgg atcgtcgacc tggcccgtga
      121 gatctccggc gcgcgctacg ctgccctggg cgtgccgggg ccgtctggtg agctggaatc
      181 gttcctgacg tcggggatct ccagcgagga ggtagccgcg attgcccacc gaccggaggg
      241 acgtgggctg ctcgggctgt tgctgcgcga accgggaacc gtgcggttgg cgaacctggc
      301 cgagcatccg gtgtcggtcg ggtttccggc caaccacccg ccgatgcgca gctttctcgg
      361 cgtgccgatc atggcgcatg ggaaggtgct cggcaatctc tacctgaccg agaagcggac
      421 gggcagcgcc tttaccgacg acgatgcgcg gctggtcgag gaactggccc ggcatgcagc
      481 tgtcgcgatc gagaacgccc atctgtacca gcaggtcggg cagcaacagc agcggctgca
      541 actgatcctc gaccagcttc ccgaggcgac gctgctggcc gagcgtgatc cggagcgggt
      601 cacgctcgcc aatcagcacg cgtccaacct gctcggctgg cagatcgaga caccgatccc
      661 gctggagaca ttcctgcagc ggaacccgcg cctgggccag gacggtacgc cgatgccggc
      721 tgagagcatt ccaatggtgg agtcactgcg gaccggcgag cgcatcagtc accgcgaggt
      781 acggatcgga cgagcggacg ggcaggtgat gacggcgctc gtcaacagcg tgccgctacg
      841 cgggccggat ggtgagatca ccggcgcgat cgccgtcttt caggacatca cccagatcaa
      901 ggatgccgaa cagctgaagg acgacttcct ctcgctcgtc tcgcacgagc tgcggacgcc
      961 gctgacgacg attcagggtg gcgcgctgat gctccagcgt gactgggccg tgctggacga
     1021 cgagacccgg caggatctgc tggccgacat cgccaatgag acccggcggc tgggcgggct
     1081 gatcgagaat atggtccaac tggctaacat ccgcgccggc cggatgcgga tggagaccga
     1141 gccggttcat gtcctgcggc tcctcgacgg cgcgatcgcg gcgacccgcc agctcgatcc
     1201 ggagcgggtc gtgacgctgg cggcggacgc ggacctgttc gccgaagccg accccgaccg
     1261 gctcgaccag gtgatacgca acctgctcag caacgcgatc aagtacgcgc cgcccgggct
     1321 gccggtcgag gtgacggcga acgagcagga cgggatggtc accatcactg tccgcgacta
     1381 cggccccggc atcgccgata ccgacctgcc gcgcatcttc gaccgcttca gccgggccgg
     1441 ccgagccgtc agcagcggcg caccggggat ggggctcggc ctctatctcg tgcggcacgt
     1501 cgtcgaagcg catggcggct cgatctggat cgagcgtccg gacgacgggg gcacccgggt
     1561 ggtcctgacg atcccagcca gttgagcgcg cgggttgtaa cggaactgcg acgctgacgg
     1621 cggagttgag actcgcccgc aaccggtcat cgtccatcat caatctggtg attgcaattc
     1681 ggaggaggtt tgtcatgaag accccgcagg acctgttcga cctgaccggc aagatcgcga
     1741 tcgtcaccgg cgccggttcc ggcctcggcg ttgtctttgc tcaggcgctg gccgaggctg
     1801 gcgccggtgt tgtctgcgcc gatctccggc tggacggggc cgaggagacg gcgcgctcga
     1861 tcgaggagct tggccggccc gcactggcgg tcgagacgga tgtcaccgat gaggcggccg
     1921 tccagtcgat ggtccagaca acgctcgacc gcttcgggcg gatcgacatt ctcgtcaaca
     1981 acgccgggat cggcgtcggc gggtcgccgg aggagacgac gctggccgac tggcaggcag
     2041 ccgtcaatgt caacctgacg ggcgtcttca tctgcgcgcg cgaggtcggc aaggcgatga
     2101 tcgcggccgg caccggcggc cggatcatca acatcgcctc gatcctcggc gccgtcgcct
     2161 ccgagccggc gccggtgacg gcctacgccg cgacgaaggg cgccgtcatc aacctgaccc
     2221 gcgacctggc cgtccactgg gcgccgcacg gcatcctcgt caacgcgctc ggcccggcct
     2281 actttcccag cgcgatgacc ggcgacgccc tcgccatgcc cgaagtgatg gccgagattg
     2341 agcgtcggac gccgctcggc cgtgtcggcc ggctggagga gctgaagggg ccggtcgtcc
     2401 tgctggcgtc cgacgcaggc tcctacatca ccggccagac cttgttcgtc gatggcggct
     2461 ggacggcctg gtgatctgag cagtaacgcc gcaaccgggg ccgggagcgc tctgctccgc
     2521 aacgcgaccg gctcatcacc tggactcgcg gagcgacgtt caaccttgag ggggaggagt
     2581 cgttccaatg gagtccgaga ctactgccca tcgcccccgg ttgcatccgt tacatgcgcg
     2641 cccgcgttcg atgtcgctgc ctcgtcccgg gccgctcgtg ctaccgcgac tgacgctctt
     2701 gcttgtcctc gcgctgctcg tctgggcggg gatgcagcag ctctcgacgc cggctgtcgt
     2761 cccagcctcc gccccggcga ctgagttctc cgccgcccgg gcaatgcagc acctgcgagt
     2821 cattgcctcc gcgccgcgtg cggtcggcct ggccggtcac acggctaccc gcgagtacct
     2881 ggtggct
//
LOCUS       DASUGJ010000634         4007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_382241, whole genome shotgun sequence.
ACCESSION   DASUGJ010000634 DASUGJ010000000
VERSION     DASUGJ010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382241"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1992
                     /locus_tag="VFV93_14530"
     CDS             <1..1992
                     /locus_tag="VFV93_14530"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5166617.1"
                     /translation="VDPDTLTTGNYRAVIAEARQLQFTTGPTDTILTGGHNRITLSND
                     LPRLLNDFALQTNVALTVIALSAVGLLVTGLAALALLAALIADRRRATVALIRGRGAS
                     SAQLFAAQIAEGLLLCVPAALLGYGLALLLVDSRPNPWSVAGSLAMAVLTIALLVTAS
                     RVNISVALGTLLSRTSVHRGRVSVRRLAAEGAVVLVAVAGIVLLRRRGLAPRVSAEGD
                     VSFDPFLTAVPILLTLAVGLVALRLYPLLMRLAARIAGTGRGLVAFIGLRRVSRQSPV
                     AHLPLLVMLLAIGISIFTAVVRFSISEAQAEGAWQRVGAPYRVETPDSSLPDRLNLGA
                     IEGVTGVAEALLTPTSSGTLLAVEADDLEAMNAGTRADPVFPDSLLEIPGGVALGTER
                     NPLPAVLAGWASDDIRIGSTIPISVPARPSPVQITLRVEEVRPAFAGVSEGQPAVIVN
                     LRALQQAAPDSQIRPNLAFLDGDGLSVEQLELDIAAQTGANDRTAGGVSSYVDVDISS
                     RDETYETIHAAPLSRGVTRSFALSVAMAALYAALAIVTALTLTARARARDMSYLRTLG
                     LSQPQALRLAIIEHVPSVLIASALGAGLGIAIVRLIEPGLDVSAFVGPGTRVPLRTDW
                     TSITAITIALTLITLAAIAFFTAINLRAQISQSLRVGDS"
     gene            2117..2578
                     /locus_tag="VFV93_14535"
     CDS             2117..2578
                     /locus_tag="VFV93_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5166618.1"
                     /translation="MTPAERAQLIEQYMDGYRVITEALDEAGEAGLDAREAPDEWSPR
                     EVVHHLADSEMTSAMRIRRVLVEDNPLIQGYDQEAFANRLWYNRPIEHSLDAFRLARA
                     TTAEILTRMSDADWQRTGTHTESGPYSAEDWLRIYAVHAHEHADQIRRAVA"
     gene            complement(2676..>4007)
                     /locus_tag="VFV93_14540"
     CDS             complement(2676..>4007)
                     /locus_tag="VFV93_14540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5166619.1"
                     /translation="VRDDTWFGKYPPGYPLVLAVGVLAGAPWLVNPILAGVAVLLAFI
                     IGRRLYDAPTGLVAAALLAISPFLMLQAGSLMSHVAALVWTLLALLAFESVARRSATW
                     PAVGCGAALGMLALTRSLTAAGIGLPLGIWLLARIVHDRRNWRAAACIVAGALPFVVA
                     LLAYNQLTTGSPFRTGYELWWPWDRIGFGEGVSRDGNYTLHEAWDNMRSNAGDLAMYL
                     YGWPLRLSLLPAIAAAALAIRRALGWWRDPLRRPIRRDRWANQWDLLLVGTVVSLIGV
                     HVVYWTDGQMYGPRYYFEIIGALALLSARAFVALAAYLAERLRRNGLTWQTAQATSSL
                     AILLVVTLLSLHSARNFSQARFDEFHNWYGINRDGIETVQSANLSNAIVFVTAPSWTV
                     YAPFFLESAPALDGDVIYAIDRGPDANRRLLADFPGREAYVFADGRVRGFR"
BASE COUNT          638 a         1439 c         1329 g          601 t
ORIGIN      
        1 gtcgatcccg acacactgac gaccggcaac taccgggccg tcatcgccga agcgcgtcag
       61 ctccagttca cgacggggcc gaccgacacg atcctcaccg gcggccacaa ccgcatcacc
      121 ctcagcaacg acctgccccg gctcctcaac gatttcgcgc tccagaccaa cgtcgcgctg
      181 accgtcatcg ccctctccgc cgtcggcctg ctggtgaccg gactggccgc gctggcgctg
      241 ctggcggcgc tgatcgccga ccggcgacgg gcgacggtcg cgctgattcg cgggcggggc
      301 gcgtcctcgg cccagctctt cgcagctcag atcgccgagg gcctgctgct ctgcgtcccg
      361 gccgcgctgc tgggctacgg gttggcgctg ctgctcgtcg actcgcggcc caacccgtgg
      421 tcggtggccg gcagcctggc gatggccgtc ctgacgatcg cgctgctggt gacggcctcg
      481 cgggtgaata tcagcgtcgc gctcgggacg ctgctgagtc gcaccagcgt ccatcgcggt
      541 cgcgtgtcgg ttcgccggct ggcggcggaa ggcgcggtcg tcctggtcgc ggtcgctggg
      601 atcgtcctgc tgcggcgacg tgggctggcg ccacgagtca gcgccgaggg cgatgtctcg
      661 ttcgacccat tcctgacagc cgtcccgatc ctgctgacgc tggcggtcgg gttggtcgcc
      721 ttgcggctct atccgctcct gatgcggctg gcggcgcgga tcgccgggac cggacgcggg
      781 ctggtcgcct tcatcggtct gaggcgggtc tcgcgccagt cgccggtcgc ccacctgccg
      841 ctgctggtga tgctgctggc gatcggcatc tcgatcttca ccgccgtcgt ccgcttctcg
      901 atctccgagg cgcaggccga gggtgcctgg caacgggtcg gcgcgcccta ccgggtcgag
      961 acgcccgaca gcagcctgcc cgaccggctc aacctcggcg cgatcgaggg cgtcaccggc
     1021 gtcgctgagg cgttgctgac gccgaccagc tccggcacgc tgctcgccgt cgaggcggac
     1081 gatctggaag cgatgaacgc cgggacgcgg gccgacccgg tcttccccga cagcctgctg
     1141 gagatccccg gtggcgtcgc gctcggcacc gaacgcaacc cgctgccggc cgtcctcgcc
     1201 ggctgggcgt ccgacgatat ccgaatcggc agcaccatcc cgatctcggt cccggcccgg
     1261 ccgtcgccgg tccagatcac gctgcgggtc gaggaagtcc ggcccgcctt cgccggtgtt
     1321 tccgaggggc agccggccgt catcgtcaac ctgcgtgcgt tgcagcaggc ggcccccgac
     1381 tcgcagatcc gccccaacct ggccttcctc gacggcgacg ggctcagtgt cgagcagctt
     1441 gagctggaca tcgccgccca gaccggcgcc aacgaccgga cggccggtgg ggtcagctcg
     1501 tatgtcgacg tcgacatctc ctcgcgcgac gagacgtacg aaacgatcca tgccgcacca
     1561 ctctcacggg gcgtcacccg cagcttcgcg ctcagcgtcg caatggccgc cctctacgcc
     1621 gccctcgcga tcgtgacggc cttgacgctg acggcccgtg cccgcgcccg cgatatgtcc
     1681 tacctgcgca cgctcggcct ctcgcagccc caggcgctcc ggctagccat catcgagcac
     1741 gtgccgtcgg tcctgatcgc ctccgcgctc ggcgccgggc tgggcatcgc gatcgtccgc
     1801 ctgatcgagc cggggctgga tgtctccgcc ttcgtcggcc ccggcacgcg cgtcccgctc
     1861 cggaccgact ggacctcgat caccgccatc acgatcgcgt tgacgctcat cacgctggca
     1921 gccatcgcct tcttcactgc catcaacctc cgcgcccaga tcagccagtc attgcgggtc
     1981 ggcgacagtt gatagcggca cactccaacg cgccgttcca ccagcgaatt ggccctagcc
     2041 ggttaggtcg cctgatgcga gagtacttcg aacgattcgc cgccgttacc ccgatcgcga
     2101 aaggatgtct ggccgaatga cacccgccga gcgcgcgcaa ctgatcgaac agtacatgga
     2161 tggctaccgc gtcatcaccg aggcgcttga cgaagccggt gaagctggcc tggacgcacg
     2221 cgaggcgccc gacgagtggt cgccgcgcga ggtcgtccac cacctggccg acagcgagat
     2281 gacctccgcc atgcggatca ggcgcgtgct ggtcgaggac aacccgctga tccagggcta
     2341 cgaccaggaa gccttcgcca accgcctctg gtacaaccgc ccgatcgagc actcgctcga
     2401 cgccttccgg ttggcgcgag cgacgactgc cgagatcctg acgcgcatgt ccgacgctga
     2461 ctggcaacga accggtaccc acaccgaatc cggcccctac agcgccgaag actggctgcg
     2521 catctacgcc gtccacgccc acgagcacgc ggaccagatt cgccgggcag tggcgtaggt
     2581 ttcaggtgat aggccatagg tgttaggaat cggttcgtgc gggtgaaacg cgtcacgctg
     2641 cttcccacga accagctcct aacacctttg gcctatcacc tgaaacctcg cacgcgtcca
     2701 tccgcgaaca cgtacgcctc gcggccgggg aagtcggcta gcagccggcg gttggcgtcc
     2761 gggccgcggt cgatggcgta gatgacgtcg ccgtcgaggg cgggggcgga ttcgaggaag
     2821 aacggggcgt acaccgtcca gcttggggcc gtgacgaaga cgatcgcgtt gctcaagttg
     2881 gccgattgaa cggtttcgat cccgtcacgg ttgatgccgt accagttgtg gaactcgtcg
     2941 aagcgtgcct ggctgaagtt gcgggcgctg tgaagcgaga gcagtgtcac caccagcagg
     3001 atcgccagtg agctggttgc ctgggcggtc tgccacgtca ggccatttcg gcgcagccgt
     3061 tcagcgaggt aggcagccag ggcgacgaac gcccgcgccg agagcaacgc cagcgccccg
     3121 atgatctcga agtagtagcg cgggccgtac atctgcccgt cggtccagta gacgacatga
     3181 acgccgatca ggctgacgac cgtcccgacc agcagcagat cccactggtt ggcccagcgg
     3241 tcgcgccgga tcggccgacg gagcgggtca cgccaccagc cgagggcacg ccggatcgcc
     3301 agagcggcag cggcgatggc cggcagcagg ctaagccgca gcggccagcc gtagagatac
     3361 atcgcgaggt caccggcgtt ggaccgcatg ttgtcccacg cctcgtgcag cgtgtagtta
     3421 ccgtcacgtg agacgccttc gccgaagccg atccggtccc acggccacca gagctcgtag
     3481 ccggtccgga acgggctgcc ggtcgtcagc tggttgtagg ccagcagcgc gacgacgaac
     3541 ggcaacgcac cggcaacgat gcaggcagct gcgcgccagt tgcgccggtc gtggacgatc
     3601 ctcgcgagca gccagatccc aagcggcagg ccgatcccgg ccgccgtcag cgagcgggtc
     3661 agcgccagca tccccagcgc cgcaccgcag ccaaccgccg gccaggtcgc actacggcgg
     3721 gcgacggact cgaacgccag cagcgccagc agcgtccaga ccagcgcggc gacgtgcgac
     3781 atcagcgagc cggcctggag catcagaaac ggcgagatcg ccagcagcgc ggcggcgacg
     3841 aggccggtcg gagcgtcgta gagccgacgg ccgatgatga acgccagcag gacagcgaca
     3901 ccggccagga tcgggttcac cagccatggc gcaccggcca gaacgccgac cgccagcacc
     3961 agcgggtatc cgggcgggta tttgccgaac cacgtgtcgt cgcggac
//
LOCUS       DASUGJ010000635         9084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_659583, whole genome shotgun sequence.
ACCESSION   DASUGJ010000635 DASUGJ010000000
VERSION     DASUGJ010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659583"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1033
                     /locus_tag="VFV93_14545"
     CDS             191..1033
                     /locus_tag="VFV93_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5166620.1"
                     /translation="MSQDPARKDPNTPSEAVATGAESTTVGPKDDPASTPTTDNQRMQ
                     AEASPAGDVSDAADPDTSDARAALAQMSDPSVPVIEPAHDMTGVQVIDPVWVHSGYWG
                     PLDESIHPAVVALRAALPDAVKEVTFFRDEVTVHVVADRLVEVATLLRDSDDLAYRLL
                     SDLTAVDMLRLRRSPRFDVVVTLYSVKNRKRLRLKAAVEESQPCPSLTSVYKAAGWLE
                     RETYDMFGIRFLGHPDLRRMLLPEDWDEGHPLRKDYPIRGYKQYVQPGFENPAPRIRE
                     PRRP"
     gene            1036..2280
                     /gene="nuoD"
                     /locus_tag="VFV93_14550"
     CDS             1036..2280
                     /gene="nuoD"
                     /locus_tag="VFV93_14550"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX5166621.1"
                     /translation="MSAIDMEPQSAYGTLTVEDEHQLSDTRRQLVMNMGPQHPSTHGV
                     LRVVVTLEGEVVVGTDPIIGYLHRGVEKIGETRRYSQFTPWTDRTDYVAAPLNNLGYI
                     LTVEKLVGITATERAQYWRVIMAELSRIASHLVWLGTHALDIGALSMSLFCFREREMI
                     LDIFEKFCGARLTTNMMEIGGFSRPIPPGLVEMIEDFIKIFPTMQQEYVDLLTDNPIW
                     LARTRGVGVIEPQVALDYGLTGACLRGSGINYDIRKAQPYLVYDRIDFEVPIGTHGDV
                     FDRYLVRMEEMRQSLRIIRQCLDQVPTDGPLMRHDTNYSIPPHDTVLRTAEDMQRHFV
                     WVVKGFNAPKGDVYVAIEHSKGELGHYIVSDGTAVPYRLRIRTPDFVNLQVLPHMAQG
                     AMLADMVALIGTIDIVLGSVDR"
     gene            2547..3680
                     /gene="nuoH"
                     /locus_tag="VFV93_14555"
     CDS             2547..3680
                     /gene="nuoH"
                     /locus_tag="VFV93_14555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX5166622.1"
                     /translation="MTDQPFWITFIVGFIVLNLLLVGMAYMTWLERKVLARMQGRFGP
                     NRAGPWGLLQPIADGIKLIGKEDLVPKFADKRIFIIAPLITFVCAPLGGLVIPFGDTI
                     TIAGQDINLFVTDMNVAVLYMLAVSSVGVYGIILGGYASANRYSLLGALRSAAQVISY
                     EIVLGLSLVGVFILSSSLSLVTIVNEQRRPLDFSLGGLDFSIPNWYIFSQPVAFIIFL
                     IAAVAETNRAPFDLPEAETELISGFHTEYSGFRFSFYFLGEYISMIVISLLAASLFLG
                     GIDGPIKDGFWWLLLKMSIFLFFYIWLRATLPRFRYDQLMGIAWKVMLPLTLVNILLT
                     GLIRLRSNDDISLQAFLITFAVIVVAVAAIFFASSRKQEAHVG"
     gene            3670..4473
                     /locus_tag="VFV93_14560"
     CDS             3670..4473
                     /locus_tag="VFV93_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX5166623.1"
                     /translation="MSAEVLVFDILAVLAVIAGLGVVTLRNPVHSAVSLIVTFINLAG
                     IFVMLRAEFLAVVQIIVYTGAILVLVLFVIMLVDPDDLPEFHGGQPLQQAAGFIIGLM
                     LLGEVAAAVLTRTVVGQPGPWNEDFVQQQGGNVQVLGQFLYSGYILPIQAVAVVLLVG
                     TIGALVLARPDPPSVLPRQRKIGTISLGHPRGAEGEFYPPLPLGAGVGAGAAATTARG
                     EMRNGLIFVKDADAYSDRPAWELEFSEPEPDDKSYAHDGFGIGREGERS"
     gene            4470..4778
                     /gene="nuoK"
                     /locus_tag="VFV93_14565"
     CDS             4470..4778
                     /gene="nuoK"
                     /locus_tag="VFV93_14565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX5166624.1"
                     /translation="MSELTAEHFLLLSGALFIIGTIGVLTRRNVLIIFMSVELMINAV
                     NVAFVGFAWEQDSMIGQTFALFIIAVAAAESILGLGIIMALSRRIDSVDVADVTTLRD
                     "
     gene            4914..6890
                     /gene="nuoL"
                     /locus_tag="VFV93_14570"
     CDS             4914..6890
                     /gene="nuoL"
                     /locus_tag="VFV93_14570"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX5166625.1"
                     /translation="MEDLLVLIPLLPLVAALINFLFGRWFIRDLAGPIASVAVFGSWV
                     LSLLVFIDQIGSDEPLAQHLYTWMPAGDFHVPMNLYVDHLSATMLMVVTTVSTLVHIY
                     AIGYMHGDPGFYRFFSYLPLFVFSMLMLVLADNYLMLFIFWEAVGLCSYLLIGYYFKR
                     KSANDAAKKAFIVNRIGDFGFGIGVMLIFVKTGSIVFDEVFARIPEVGSTAVFWIAVL
                     LFMGACGKSAQIPLFVWLPDAMEGPTPVSALIHAATMVTAGVYMVARSFPIYDLTDDA
                     RLIVSIVGAATAIVAATIGVTQFDIKRVIAYSTVSQLGYMVFALGVGAWIPAIFHLVT
                     HAFFKGLLFLGSGSVIHGMHDEQDMRNMGGLKKYMPITYWTFLIAALANAGIFPLAGF
                     WSKDEIILGSWVEGYEWVAVVGFLAAFFTALYMARVIYLTFHGEERFDSHQIHPHESP
                     RVMAVPLILLAIPAAVIGLGLGWPPEKGWIHDFLEPNFHSEAADAHALVAIGPDVTFA
                     AQETEAAAEEHHVSNAETITFGIISTIIAGGGWLIAYAAYIRKVPALSPVLWAARFGS
                     FYTFVSRKWMFDEFYETFIVHPMYLFSRFLWRVVDVGIIDGTVNGVAGAVGFTSSRLR
                     RVQTGFVANYALAMALGAVIIVAAFYVFQSSLFA"
     gene            7052..8716
                     /locus_tag="VFV93_14575"
     CDS             7052..8716
                     /locus_tag="VFV93_14575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX5166626.1"
                     /translation="MDDFPILSVIAYLPLLGALIIFFWPGATPNQTRQVALWSSIAAF
                     VASVVMLIGFDPDKSGMQWNEHMTWLPDLGINYSLGVDGVSVFLIVLTTLLSVVSVLA
                     SFGPIQHRVREYFIALLLLEVGMVGVFVALDLFLFYIFFELTLIPMALLIGVWGSANR
                     VYAAIKFFLYTLAGSLLMLVGIVATYQSYYDQTGIRSLNILDLQQGNYGHTFQLWVFA
                     AFFIAFAIKIPMWPFHTWLPDAHVEAPTAGSVLLAGVLLKIGGYGMIRFNLSLFEDAS
                     QDYAPWIIALSVIAIIYGALVALPQPDMKKLIAYSSVSHMGFVTLGIFAFNMQGLYGA
                     MIVMLAHGLNTGALFLCVGVLYNRAHTRLISAFGGLSQRMPIYSTIFCVFTFASIGLP
                     GLSGFIGEFLSVLGAFREYRWAAIISFTVVIFSAWYMLWLFQRVVFGRAPGELPDPGD
                     NALTDDERAMLAAHGGHDHGHGHAVPAVSGGSGLDEDDHGHAAHADVDQDGRALHMPD
                     LNTNELWTLVPLLVLTIALGVYPGPVMDYVKATFEAILVPFGGTGV"
     gene            8799..>9084
                     /locus_tag="VFV93_14580"
     CDS             8799..>9084
                     /locus_tag="VFV93_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166627.1"
                     /translation="MPQIFEQVNPDLTLAIPQLIVLGLALLLLLADVVIPRARHGILT
                     WITLAGFAAALVATLYYVYDNTNDSTFTTPGYGDMFLTDGFSIFISLVVLT"
BASE COUNT         1490 a         2900 c         2783 g         1911 t
ORIGIN      
        1 cgaccggatc gctgcccggc aaacgatcgc cggcaccgat gcgatgatcg tcagccagtc
       61 ggccggcttc ggtgagccga gcgacaacag cttcccgcgc gcgtcgtagc gcacgggcac
      121 atgcgcggcg cgggtcgcgc aaccggaggg cgcctgacgc ccgtctgagc cagcaggagg
      181 cggattcggc atgtcccagg accccgcccg caaggacccc aacacgccgt ctgaggccgt
      241 cgcgaccggt gcggaatcga caaccgtcgg cccaaaggac gacccggcaa gcacgccaac
      301 gaccgataac cagcgcatgc aggctgaggc gtcgccggcc ggcgatgtct ccgacgccgc
      361 cgatcccgat acaagcgacg cccgcgccgc gctggcgcag atgagcgacc cgtcggtgcc
      421 ggtcatcgag ccggctcacg acatgaccgg cgtgcaggtc atcgacccgg tctgggttca
      481 ctcaggctac tgggggccac tggacgagag catccacccg gccgtcgttg ccctgcgggc
      541 agcgctgccg gacgccgtca aggaagttac gttctttcgt gatgaggtaa ccgtccacgt
      601 cgtcgccgat cggctggtcg aggtggcgac gctgctgcgc gactctgacg atctcgccta
      661 ccgcctgttg agcgacctga cagcggttga catgcttcgg ctacgccgtt cgccacggtt
      721 tgatgtcgtc gtcacgctct actcggtcaa gaaccgcaag cggctgcgtt tgaaggcggc
      781 cgttgaggaa tcgcagccgt gcccgtcgct gaccagtgtc tacaaggcag ccggctggct
      841 ggagcgtgag acctacgaca tgttcgggat caggttcctc ggtcacccgg acctgcgccg
      901 gatgctgttg ccggaggact gggacgaggg acacccgctg cgcaaggact acccaattcg
      961 cggctacaag cagtatgtcc agccagggtt cgagaaccct gctccacgca ttcgcgagcc
     1021 acggaggccg taacaatgtc tgccatcgac atggaaccgc agtctgcata cggaacactg
     1081 acggtcgagg atgagcatca gctctccgac acacgccgcc aactcgtcat gaacatgggg
     1141 ccgcagcacc cctcgacgca cggcgtgttg cgcgtcgtcg tcacgctcga aggcgaagtg
     1201 gtcgttggga cggacccgat catcggctac ctgcatcgcg gcgttgagaa gatcggcgag
     1261 acacggcgct actcgcagtt cacgccctgg accgatcgga ccgactatgt cgcggcgccg
     1321 ctcaacaacc tcggctacat cctgacggtc gaaaagctgg tcgggatcac cgccaccgag
     1381 cgggcgcaat actggcgcgt catcatggcc gagctgtcgc ggatcgccag ccacctcgtc
     1441 tggctcggca cgcacgcgct cgacatcggc gcgctctcga tgagcctgtt ctgcttccgc
     1501 gagcgggaga tgatcctcga catcttcgag aagttctgcg gtgcccgact gacgaccaac
     1561 atgatggaga tcggcggctt ctcgcgtccg atcccgcccg gcctggtcga gatgatcgaa
     1621 gacttcatca agatcttccc gaccatgcag caggagtacg tcgatctgct taccgacaac
     1681 ccgatctggc ttgcgcggac gcgaggcgtc ggggtgatcg agccgcaggt tgcgctcgac
     1741 tatggcctga ccggcgcctg cctgcgcgga agcggcatca actacgacat ccgcaaggcg
     1801 cagccctacc ttgtttacga ccgcatcgac ttcgaggtgc cgatcggcac ccacggcgat
     1861 gtcttcgacc gctatctcgt ccggatggaa gagatgcgcc agtcactacg gatcatccgt
     1921 caatgcctcg accaggtccc gaccgacggc ccgctgatgc gacatgacac caactacagc
     1981 attccgccac acgacacggt cctgcgaacg gccgaagaca tgcagcggca cttcgtctgg
     2041 gtcgtcaagg gtttcaacgc gccgaagggc gatgtctacg tcgcgatcga gcactcaaag
     2101 ggtgagctgg gccattacat cgtgtccgat gggacggcgg ttccctaccg gctgcggatt
     2161 cggacgccgg acttcgtcaa cctgcaggtg ctaccgcaca tggcgcaggg agcgatgctg
     2221 gctgacatgg tcgcgctgat tgggacgatc gatatcgtgc tcggctctgt cgaccgatga
     2281 cgagttgaac gtgctacgcg ctgagttgaa tgtggactgg atcggtggcg tccacgctcg
     2341 aaggtccagg gcgaacttct ttcaactcag cacgtagcac tttcaactcg tactgaatcg
     2401 ggtacactca gttgttgtga cggtgacgcg ttggggcagc cgtcgcaaga cccacggggt
     2461 gggtggggaa cgtccggggc gcttggggaa gcgacggacg aggcggcgaa tgccgccgta
     2521 ttcgtgtgag ggggaaccac gtcgggatga ccgatcaacc gttctggata accttcatcg
     2581 ttgggtttat cgtcctgaac ctgctgctgg tcggcatggc ttacatgacc tggctggagc
     2641 gcaaggttct ggcacgcatg cagggacggt ttggacccaa ccgcgccggg ccgtgggggt
     2701 tgctacagcc gattgccgac ggcatcaagc tgatcggcaa agaggacctg gttcccaagt
     2761 tcgccgacaa gcgcatcttc atcattgcac cgctgatcac cttcgtctgc gcgcctctgg
     2821 gtggcctcgt catcccgttc ggcgacacga tcacgatcgc cggccaggac atcaacctct
     2881 tcgtgactga catgaacgtg gccgtcctct acatgctggc ggtcagttcg gtcggggtct
     2941 acggcatcat cctcggcgga tatgcgtcgg ctaatcgcta ctcgttgctc ggcgcgctgc
     3001 gctcggccgc acaggtgatc tcgtacgaga tcgtgctcgg gctctcgctg gtcggcgtct
     3061 tcatcctctc gtcgtcgctg tcgctggtca cgatcgtcaa tgagcagcgc cggccgctcg
     3121 atttcagtct cggcggtctc gatttctcga ttcccaactg gtacatcttc tcccagccgg
     3181 tcgccttcat catcttcctg atcgcggccg ttgccgaaac gaatcgggct ccgtttgacc
     3241 tgccggaagc cgagacggaa ctgatctccg gcttccacac cgagtattcc ggcttccgct
     3301 tctcgttcta cttcctcggc gaatacatca gcatgatcgt catctcgtta ctggcggcat
     3361 cgctcttcct cggcggcatc gatggcccga tcaaggacgg cttctggtgg ctgctgttga
     3421 agatgagcat cttcctgttt ttctacatct ggctgcgcgc cacgctcccg cgcttccgct
     3481 acgaccagct gatgggtatc gcctggaagg tgatgctgcc gttgacgctg gttaacatcc
     3541 tgctgaccgg cctgattcgc ctgcgctcga acgatgacat ctccctgcag gcattcctga
     3601 ttaccttcgc ggtgatcgtc gtcgcggtcg cggcgatctt cttcgccagt tctcggaagc
     3661 aggaggcgca tgtcggctga ggtactcgtg ttcgacatcc tggcggtcct ggcggttatc
     3721 gccggcctcg gggtcgtcac gctgcgtaat ccggttcaca gcgccgtctc gctgatcgtg
     3781 accttcatca atctggccgg catcttcgtg atgttgcggg ccgagttcct ggcggtcgtc
     3841 cagatcatcg tctacaccgg cgcgattctg gtgctggttt tgttcgtgat catgctggtc
     3901 gatccggatg atctgccgga gtttcacggc ggccagccgc tccaacaggc agccggtttc
     3961 atcatcggcc tgatgctgct cggcgaggtg gccgccgccg ttctgacgcg gaccgttgtc
     4021 ggccagccag ggccgtggaa cgaggacttc gtccagcagc agggcggcaa cgtccaggtg
     4081 ctgggtcagt tcctctactc cggctacatc ctgccgattc aggcggttgc cgtcgtcctg
     4141 ctggtcggca cgatcggcgc gctggtgctc gcccggcccg atccgccgtc ggtcctgcca
     4201 cggcagcgca agatcggcac gatctcgctc ggccatccgc gaggtgctga gggtgagttc
     4261 tacccgccgc tcccgctcgg tgcgggcgta ggcgccggtg cagccgcgac aacggcacgc
     4321 ggcgagatgc gcaacgggct gatcttcgtc aaggacgccg acgcctacag cgaccgtccg
     4381 gcctgggagc ttgaattctc cgagccggag ccggatgaca agagttacgc ccacgatggt
     4441 ttcggcatcg gcagggaagg agagcgatcg tgagcgagct gacggccgag cactttctgc
     4501 tcttgagtgg cgcgctgttc attatcggca cgatcggggt cctcacccgg cgcaacgtcc
     4561 tcatcatctt catgagcgtc gagctgatga tcaacgccgt caacgtcgcc ttcgtcgggt
     4621 tcgcctggga acaggactcg atgatcggcc agacctttgc gctcttcatc atcgctgtcg
     4681 ccgccgccga atcgatcctc ggcctcggca tcatcatggc gctctcgcgc cggatcgatt
     4741 cggttgacgt cgctgacgtg acgacgctgc gggattaggg gacgagtgct gagtgctggg
     4801 ttctgagtac tgattattga gttgcgaggg tccgcgtccg ggcaccgttc ggacatcgga
     4861 tcgtctactc agcactcagc actgagcact cagcacccgc aagagggagc accatggagg
     4921 acttgctggt attgatcccg ctgctgccgc tggtggctgc gttgatcaat ttcctgttcg
     4981 ggcgctggtt tatccgcgac cttgccggac cgatcgcatc ggtcgcggtc ttcgggtcgt
     5041 gggtgctgag cctgctggtg ttcatcgatc agatcggctc ggacgagccg ctggcccagc
     5101 acctctatac ctggatgccg gccggcgatt tccatgtgcc gatgaacctg tacgtcgatc
     5161 acctgtcggc cacgatgctg atggtcgtca cgaccgtctc gacactggtc catatctacg
     5221 ccattggcta catgcacggc gatcccggct tctaccgctt cttctcctac ctgccgctgt
     5281 tcgtcttctc gatgctgatg ctcgtcctgg ccgacaatta cctgatgctg ttcatcttct
     5341 gggaggccgt cggtctctgc tcttacctgc tgatcggcta ctacttcaag cgcaagtcgg
     5401 ccaatgacgc cgccaagaag gccttcatcg tcaaccgcat cggcgacttc ggtttcggga
     5461 tcggcgtgat gctgatcttc gtcaagaccg gctcgatcgt cttcgacgag gtgttcgcca
     5521 gaattcccga ggtcggtagt acagcagtct tctggatcgc ggttctgctc ttcatgggcg
     5581 catgcgggaa gtcggcccag atcccgctgt tcgtctggtt gcctgacgcg atggagggtc
     5641 cgacgccggt ctcggccctg atccacgctg cgacgatggt gacggccggt gtctacatgg
     5701 tggcgcgctc attcccgatc tacgacctga ccgacgacgc ccggctgatt gtctcaatcg
     5761 tcggcgcggc gaccgcgatc gtcgccgcga cgatcggcgt gacccagttc gacatcaagc
     5821 gcgtcattgc ctactcgacc gtctcgcagc tgggctacat ggtcttcgcg ctcggcgtcg
     5881 gtgcctggat cccggccatc ttccacctcg tcacgcacgc gttcttcaag ggtctgctct
     5941 tcctcggctc cggctccgtc atccacggga tgcacgacga gcaggacatg cgcaacatgg
     6001 gcggtctcaa gaagtacatg ccgatcacct actggacctt cctgatcgcc gcgctggcta
     6061 atgccggtat cttcccgctg gctggcttct ggagtaagga cgagatcatc ctcggctcct
     6121 gggtcgaggg ctacgagtgg gtcgcggtcg tcggcttcct cgcggccttc ttcacggcgc
     6181 tctacatggc ccgcgtcatc tatttgacgt tccacggcga ggaacgtttc gactcccacc
     6241 agatccaccc gcacgaatca ccgagggtga tggcggttcc gttgatcctg ctggcgatcc
     6301 cggcagcggt gatcgggctc gggctgggct ggccgcctga gaagggctgg attcacgact
     6361 tcctggagcc gaacttccac agtgaagcag ccgacgcgca cgcgctggtc gcaatcggtc
     6421 ctgacgtcac cttcgccgcc caggagaccg aggcggcggc cgaggagcat cacgtctcca
     6481 acgcggagac gatcaccttc ggcatcatct cgacgatcat cgccggcggc ggctggctga
     6541 tcgcctatgc cgcctacatc cgcaaggttc ccgcactcag cccggtactc tgggcggcgc
     6601 gcttcggtag cttctacacc ttcgtctcgc gcaagtggat gttcgacgag ttctacgaaa
     6661 cgttcatcgt ccacccgatg tatctgttct cccgcttcct ctggcgggtc gtcgacgttg
     6721 gcatcatcga tgggaccgtc aatggcgtcg ccggagcggt cgggttcaca tcatcgcgac
     6781 tacggcgggt acagaccggt ttcgtcgcca actatgcgct ggcgatggcg ctgggagccg
     6841 tcatcatcgt agcggcgttc tatgtcttcc agagcagcct gtttgcgtag cgtcgggatc
     6901 ggggaatcgg gattcggggg tagtaacgcg gacatgagca tgcgagaaca ggttcgcata
     6961 atcgcagacg agtcgggatg ttcatgcgga cagcggggcg cgatcgttcc cgagtcccag
     7021 ttcccgaatc ccgacccgaa gggatcgaac cgtggatgat ttcccgattc tgagtgtcat
     7081 cgcgtacctg ccgctgctgg gtgcgctgat catcttcttc tggccggggg cgacgccgaa
     7141 tcagacgcgg caggtcgcgc tctggtcgtc gatcgctgcg ttcgttgcct cagtcgtgat
     7201 gctgatcggg tttgaccccg acaagtccgg catgcagtgg aacgagcaca tgacctggct
     7261 gccggatctt ggcatcaact actcgttggg tgttgatggc gtctcggtct tcctgatcgt
     7321 cctgacgacc ctgttgagcg tcgtctcggt cctggcctcg ttcggtccga tccagcaccg
     7381 tgttcgtgag tatttcatcg cgttgctgct gctcgaagtt ggcatggtcg gcgtcttcgt
     7441 agcgctcgac ctgttcctgt tctatatctt cttcgagtta acgctgattc cgatggcatt
     7501 gctgatcggt gtctggggaa gcgccaaccg cgtgtatgcg gcgatcaaat tcttcctgta
     7561 tacgctggcc ggttcgctgc tgatgctggt cgggattgtc gcgacctacc agtcgtacta
     7621 cgaccagacc ggaatccgct cgcttaacat cctcgacttg caacagggga actacggcca
     7681 tacgttccag ctctgggtct tcgccgcctt cttcatcgcc ttcgcgatca agatcccgat
     7741 gtggccgttc cacacctggt tgcctgatgc ccacgtcgag gcgccgacgg caggatcagt
     7801 cctccttgcc ggcgtgctgc tcaagatcgg cggctacggc atgattcgct tcaacctgtc
     7861 gctcttcgag gacgccagcc aggactatgc gccctggatc atcgccttgt cggtgatcgc
     7921 catcatctac ggcgcgctgg tggccctgcc gcaaccagac atgaagaagc tgatcgccta
     7981 ttcatcagtc agccatatgg gcttcgtgac attgggcatc ttcgcattca acatgcaagg
     8041 tctctacggc gcgatgatcg tgatgctggc gcacggtctc aacaccggtg cgctcttcct
     8101 ctgcgtcgga gttctctata accgagcgca cacacgcctg atcagtgcct tcggtggcct
     8161 ttcccaacga atgcccatct actcgacgat cttctgcgtc ttcacgttcg catcgatcgg
     8221 cctgcccggt ttgtccggct tcatcggtga gttcctgtcg gtgctgggcg cattccgcga
     8281 gtaccgctgg gcggcgatca tctcgttcac cgtggtgatc ttctcggcct ggtacatgct
     8341 ctggctcttc cagcgcgtcg tcttcggccg cgcgccgggc gaactgcccg atccgggcga
     8401 caacgcgctc accgacgacg agcgggcgat gctggcggcg cacggcggcc atgaccacgg
     8461 ccacggccac gcggtcccgg ctgtctccgg cggctccggc ctcgatgagg acgatcacgg
     8521 gcacgcggcg cacgctgatg tcgatcagga cgggcgggcg ttgcacatgc ccgatctgaa
     8581 cacgaacgaa ctgtggacgc tggtgccgtt gctggtcctg acgatcgcgc tgggcgtcta
     8641 ccccgggccg gtcatggact acgtgaaggc gacgttcgag gcgattctcg ttccgttcgg
     8701 cggaaccggc gtttagggac gttctcgcgc caccgtcggt ggcacaacgc tgacaggctg
     8761 acaacctgcc tggccgatag ctaacagggg gaagttcggt gccgcagatc tttgagcagg
     8821 taaatccaga cctgacactt gcgatcccgc agctgatcgt gctcgggctg gcactactgc
     8881 tgctgctggc cgacgtcgtc ataccgcgcg cccgtcacgg cattctgacg tggatcacgc
     8941 tggccggctt cgctgccgcg ctggtggcga ccctctacta cgtctacgac aacaccaacg
     9001 actcgacctt cacaacgccc ggctacggcg acatgttcct gaccgacggc ttcagcatct
     9061 tcatcagtct cgtcgtcctg accg
//
LOCUS       DASUGJ010000636         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2050158, whole genome shotgun sequence.
ACCESSION   DASUGJ010000636 DASUGJ010000000
VERSION     DASUGJ010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050158"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VFV93_14585"
     CDS             <1..347
                     /locus_tag="VFV93_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5166628.1"
                     /translation="LDNQTVVRADQRRVVSYPGTTVRREIVSPTTNGRMIVLWVTFPP
                     GERSGVQPVHHTGEECVVVIRGTMAVQVAEDTVHLEAGDSMTFDSEMPHTFWNPSDEP
                     AEVIVAISPPRI"
     gene            complement(421..1065)
                     /locus_tag="VFV93_14590"
     CDS             complement(421..1065)
                     /locus_tag="VFV93_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5166629.1"
                     /translation="MAAGGLEVIQIAAGPIETNAFLVMDRDAGEALIIDAPPEVEIEL
                     DGEVVEHGVRPVALMITHTHWDHIGDVAAIADRFAVPVIVHEIERDRLEHPGGGPIEI
                     PPAKVDRTVREGDEIALGSHRFVVLHTPGHSPGQISLYSAADDLMFGGDTLFPNGYGR
                     VDIPGASEAETLATIRRLLTLPDAVTVYTGHGLPTTVGKERHWMELVDRTGKLL"
     gene            complement(1097..1951)
                     /locus_tag="VFV93_14595"
     CDS             complement(1097..1951)
                     /locus_tag="VFV93_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166630.1"
                     /translation="MTTLLIARLTFDEALRRKMIWAVLLLSAAFLALYAFGFQLLRDD
                     VVEFDRQRGGVPNDVLPYDVQASAMVMLGLYTVNFLAGIMTIFAAVGAIAAEIESGTL
                     HAIIPKPLARWEIIAGKYLGFLAMIAAYLVLMVTGVILISRWIGKYTPPNIGKGLLLL
                     ILVSGILLSLTMLGSTIFSTMANGVIVFMLYGMAVTGGLVEQIGNALDNNTMVTIGIL
                     TSVVVPSDSMWRLASSVVQPQFAVNLIGPNPFGTTAPPSDLAVRYAIVYCATLVIASM
                     LVFRRRDI"
     gene            complement(1948..>2706)
                     /locus_tag="VFV93_14600"
     CDS             complement(1948..>2706)
                     /locus_tag="VFV93_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166631.1"
                     /translation="RAWLLGRPLGDRDAKRKIGFLPELFRFHDWLSGEELLDLHGKLY
                     GMSAAERRRRIVEVLELVGLASRGKDLVRSYSKGMQQRVGLAQALLNDPSVVFLDEPT
                     SALDPVGRLEVRTIIRALRDDGKTVFLNSHMLSEVEMVCDRVAIINRGHVAAIGTVHE
                     LLRREPVVALVVGETGADLHRLLAPHGDVRSIERTGNRIAAHVTVSDDDAIATVVEDL
                     VRAGVPVYAVTQQGPTLEELFFEIVSADERETPR"
BASE COUNT          512 a          890 c          841 g          463 t
ORIGIN      
        1 ggctcgacaa ccagaccgtc gttcgtgccg atcaacggcg ggtcgtttcc taccccggca
       61 cgaccgtccg gcgggagatc gtcagcccga ccaccaacgg ccggatgatc gtcctctggg
      121 tcactttccc gcccggcgaa cggtctggtg ttcagccagt ccaccacacc ggcgaggaat
      181 gcgtggtcgt tatccgtggc acgatggcag tccaggttgc ggaagacacc gtccaccttg
      241 aagcaggcga cagcatgacg ttcgattccg aaatgccgca cacgttctgg aacccgtccg
      301 acgaaccggc cgaagtcatc gttgcgattt cgccaccgcg catttagtgc cgattgcagt
      361 tcgcatgaaa atcctgtcgt ccaccgctca gacggagccc gtgagagtag gacgacaggg
      421 ttagagcagc ttcccggtcc ggtcaaccag ctccatccag tggcgttcct tgccgaccgt
      481 ggtcggcagg ccgtgaccgg tgtagacggt gacggcgtcc ggcagggtca gtagccggcg
      541 gatggtcgcc agcgtttctg cttcgctggc gccggggatg tcgacgcggc cgtagccgtt
      601 cggaaagagc gtgtcgccgc cgaacatcag atcgtcggcc gcgctgtaga gcgagatctg
      661 gccagggctg tgtccggggg tgtgcagcac gacgaagcgg tggctgccga gtgcgatttc
      721 gtcgccttcc cgcaccgtcc gatcgacctt cgcgggcgga atctcgatcg gcccgccgcc
      781 gggatgctcc agccggtcgc gctcgatctc gtggacgatg acaggtacgg cgaaacggtc
      841 ggcgatggcg gccacgtcgc cgatgtggtc ccagtgggta tgggtgatca tcagcgcgac
      901 gggacggaca ccgtgctcga cgacttcgcc atccagctcg atctcgacct cgggcggcgc
      961 gtcgatgatc agcgcctcgc ccgcgtcgcg gtccatcacc agaaacgcgt tcgtctcaat
     1021 cggccccgcc gcgatctgaa tgacctccag cccaccagcc gccacgtttt ccctcctcgc
     1081 gtcccacgtc ccgaatctag atatcccgac gccggaagac cagcatcgag gcgatcacga
     1141 gtgtagcgca gtagacgatc gcgtagcgga ccgccagatc gctcggcggg gccgtcgtcc
     1201 cgaaggggtt cggtccgatc aggttgacgg cgaactgcgg ctgcacgacc gacgatgcca
     1261 gccgccacat gctgtcgctg ggaacgacga cgctcgtcag gatgccgatc gtcaccatcg
     1321 tgttgttgtc aagcgcgttg ccgatctgtt cgaccagccc gccagtcacc gccatcccat
     1381 acaacatgaa gacgatcacg ccattcgcca tcgtcgagaa gatggtcgag ccgagcatcg
     1441 tcaggctgag caggatgccg gagacgagga tcagcagcag cagtcctttc ccgatgttcg
     1501 gaggcgtgta cttaccaatc cagcgggaga tgaggatgac accggtcacc atcagcacga
     1561 gataggcggc gatcatcgcc aggaagccga ggtacttgcc ggcgatgatc tcccaacgcg
     1621 ccagcggctt cgggataatg gcgtggagcg tcccggactc gatctcggct gcgatcgcgc
     1681 cgactgccgc aaagatcgtc atgatcccgg ccaggaaatt gaccgtgtaa agtccgagca
     1741 tcaccatcgc gctggcctgg acatcgtagg gcagcacgtc gttggggacg ccgccccgct
     1801 ggcggtcgaa ctcaacgacg tcgtcgcgca gaagctggaa tccgaacgcg tagagagcga
     1861 ggaaggcggc actcagtagc aggacggccc agatcatttt gcggcgtagc gcctcatcga
     1921 acgtcaaccg ggcaatcagc aacgtcgtca tcgcggcgtt tctcgctcat ctgccgacac
     1981 gatctcgaag aacaactcct caagcgtcgg gccctgctgc gtgacagcat agaccggcac
     2041 tccagcgcga accagatctt cgacaaccgt cgcgatggcg tcatcgtccg aaacggtgac
     2101 atgggcagcg attcgattcc cggttcgctc gatgctccgc acgtctccat gcggtgccag
     2161 cagtcgatgg agatcggccc ccgtctctcc gacgaccagc gcaacgaccg gctctcggcg
     2221 caacagttcg tggacagtcc caatcgccgc gacatgcccg cggttgatga tggcaactcg
     2281 gtcgcagacc atctcaacct cggagagcat gtgcgagttg aggaagacgg tcttcccgtc
     2341 gtcacgcagc gcgcggatga tggtgcggac ttcaagccgg ccgacgggat cgagcgccga
     2401 tgtcggctca tcgaggaaga cgaccgacgg atcgttcagc agcgcctgcg ccagcccgac
     2461 ccgttgttgc atgcccttcg agtacgagcg caccagatcc ttgccacgac tcgccaggcc
     2521 aaccagctcc agcacctcca ctattcggcg gcgtcgctcg gcggcgctca tcccgtagag
     2581 cttgccgtgc aggtcgagca gttcctcgcc gctgagccag tcgtgaaagc gaaacagctc
     2641 cggcagaaat ccgatcttgc gcttcgcatc gcgatcgccg agcggccggc caagcagcca
     2701 ggcccg
//
LOCUS       DASUGJ010000637         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1772364, whole genome shotgun sequence.
ACCESSION   DASUGJ010000637 DASUGJ010000000
VERSION     DASUGJ010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772364"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFV93_14605"
     CDS             complement(<1..262)
                     /locus_tag="VFV93_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166632.1"
                     /translation="MGEDIASAVSLIVSAVMLVGITAFLSLWVRRANEDELEGKRATP
                     MGLYIVFGAFGFFLFLYGLGSSYRDWREGVSPNDASYLALAVG"
     gene            415..1296
                     /locus_tag="VFV93_14610"
     CDS             415..1296
                     /locus_tag="VFV93_14610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX5166633.1"
                     /translation="MSSRRGGLGRGLESLIPGIGADTERGATSSVDIDALEPNPLQPR
                     VRWDDDQLNALAASIREHGIIQPILVTRRGTARPFQIIAGERRWRAAQRAGLTSVPIL
                     VREATPSQVLELALVENIQRADLNAIEEALAYRQLTVEFGLKQSEVAERVGKSRPAIA
                     NAIRLLSAPEAIREAVANEQISAGHARALLTIDDPLIQQQIMEQVIRLGLNVRETERL
                     AQRVISAARREPAPTRVDPTLQSVQTSLQRRLGTKVDISRGRSGGKIVISFHSDDELT
                     AILAQIAGDDDLEDYDV"
     gene            complement(1293..>2385)
                     /locus_tag="VFV93_14615"
     CDS             complement(1293..>2385)
                     /locus_tag="VFV93_14615"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX5166634.1"
                     /translation="DRPAEGLSQAEMTRIIQETPIVCPNCGKSNWTPVRAFNMMFRTQ
                     IGVVDETATTIYMRPETAQAIFVQYKNVMQSSRVKIPFGIAQIGKVFRNEITPGNFIF
                     RLLEFEQMELEYFIEPEGWEAHFDNWLEEQERFVYGLGVRPERIRRREHTAEELSHYS
                     KRTVDLEYLYPIGWKELTGLAYRYDFDLNKHQEASGEDLTYFDQANNRRYLPHVVEPT
                     MGVDRLMLMILCDAYEEEATVDAKGEQASRVVMRFHPRIAPYQVAVLPLSKKEELTVV
                     AEPLYAELRKRFSTEYDETQSIGRRYRRQDEIGTPFCVTVDFDSLNDHAVTIRERDSM
                     SQVRVPVSDVADYIEARLTEEWTPPAVLS"
BASE COUNT          445 a          818 c          703 g          419 t
ORIGIN      
        1 tgccgacggc cagtgccagg tacgacgcat cgttcgggct gacaccttcg cgccagtcac
       61 gatacgagga acccaggccg tacagaaaca gaaagaagcc gaacgcgccg aagacgatat
      121 agagacccat cggcgtcgcg cgcttgcctt caagctcgtc ctcgttcgcg cgtcgcaccc
      181 agaggctgag gaacgccgtg atgcccacca gcatgaccgc cgaaacgatc agcgataccg
      241 ccgatgcaat gtcttcaccc aattcggaaa gctccgttcg tcagcactcc ggcgaggacc
      301 ggcccagcgc gaagtatagc cagccgcctt ccgattgtga cgctttgcac agagtccgtc
      361 ccggccatcg gtatacttca tgcggagccg cgtgacagaa agtggtgagg gctggtgtcg
      421 agcagacgag gtgggctggg tcgcggactg gaatcgctga tccccggcat cggagccgac
      481 accgagcgcg gcgccaccag cagcgtcgac atcgacgcgc tcgagcctaa cccgctccag
      541 ccgcgtgttc gctgggacga cgaccagctc aacgcgctgg cggcatcgat ccgcgagcac
      601 ggcatcatcc agccgattct cgtcacccgt cgcggaactg cgcgcccatt ccagatcatc
      661 gccggtgagc gccgctggcg ggccgcccag cgcgctggcc ttacctctgt gccaatcctg
      721 gtgcgcgaag ccacaccatc gcaggtgctt gagctcgcgc tggtcgagaa cattcagcgt
      781 gccgacctga acgcgatcga agaggcgctg gcctaccgcc agctcacggt cgagttcggg
      841 ctcaagcaat ccgaggtcgc cgaacgagtt ggcaagagcc ggccggccat cgccaatgcc
      901 atccgcctcc tctccgcgcc ggaagcgatc cgcgaagctg tcgccaacga gcagatcagc
      961 gccggccacg cacgggcgct gctgacgatc gacgatcccc tcatccagca gcagatcatg
     1021 gaacaggtga tacgcctcgg actcaacgtc cgcgagacgg aacgcctcgc ccaacgcgtg
     1081 atctcggccg cccgacgcga gccggccccg acgcgagtcg atccgactct ccagtccgtc
     1141 cagacctcgc tccaacgccg gctcgggacg aaggttgaca tctcacgcgg ccgcagtggc
     1201 ggcaagatcg tcatcagttt ccactccgat gacgaactca ccgccatcct cgcccagatc
     1261 gccggcgacg acgaccttga ggattacgac gtctagctca acaccgctgg cggcgtccat
     1321 tcctcggtca gccgcgcctc gatgtagtcg gcgacgtcgc tgaccggcac ccgcacctgg
     1381 ctcatgctgt cccgctcgcg aattgtgacg gcgtggtcgt tgagcgaatc gaagtcgacc
     1441 gtcacacaga acggcgtccc aatctcatcc tgccgccggt agcgccgtcc aatgctctgc
     1501 gtctcgtcgt actcagtcga gaagcgcttg cgaagttcgg cgtagagcgg ttctgcgacg
     1561 accgtcaact cttccttctt ggacagcggc aagacggcca cctgatacgg cgcgatccgg
     1621 ggatggaagc gcatcaccac acgcgacgcc tgctcgccct tggcgtcaac cgtcgcctcc
     1681 tcctcgtacg cgtcgcagag gatcatcagc atcagccggt ccacgcccat cgtcggctcg
     1741 acgacgtgcg gcaggtaacg gcggttgttg gcctgatcga agtaagtcaa gtcttcgcca
     1801 gacgcttcct gatgcttgtt gaggtcgaag tcgtagcggt aggcgaggcc ggtcaattct
     1861 ttccagccga tcgggtagag gtactccaga tcaaccgtcc gcttcgagta atgcgacagc
     1921 tcttcggccg tgtgctcccg gcggcggatg cgttcaggcc ggacccccag cccgtagacg
     1981 aagcgctcct gctcctccag ccagttgtcg aagtgtgcct cccacccttc cggctcgatg
     2041 aagtactcga gttccatctg ctcgaactcc agcaggcgga agatgaaatt gcccggcgtg
     2101 atctcgttgc ggaacacctt gccgatctgg gcgatgccga acgggatctt gacccgactc
     2161 gactgcatca cattcttgta ctgcacgaag attgcctgtg ccgtctccgg ccgcatgtag
     2221 atcgtcgtgg ccgtctcgtc gacaacgccg atctgcgtcc ggaacatcat gttgaaggct
     2281 cgcaccggcg tccagttcga cttgccgcag ttcgggcaga caatcggcgt ctcctggatg
     2341 atgcgggtca tctcggcctg gctcaatccc tcggccggcc gatcc
//
LOCUS       DASUGJ010000638         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1910717, whole genome shotgun sequence.
ACCESSION   DASUGJ010000638 DASUGJ010000000
VERSION     DASUGJ010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910717"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..926)
                     /locus_tag="VFV93_14620"
     CDS             complement(102..926)
                     /locus_tag="VFV93_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5166635.1"
                     /translation="MYPRPFEYQQVSSVQEAIQALSGNDDAKLIAGGHSLLPVMKLRL
                     ASPSLLVDISKVAELKSVSVNGGATIGAGVTYNDLLGNAQLNASYPVLHECIASVGDV
                     QVRNRGTIGGAAAHADPAADVPAALLALGAEFTAVGPNGERTIAADDFFVDILTTALE
                     PDEVLTSIKLPAAAPKSAYEKFAHPASGYAIVGVAAVVGDGGVKVALTGATYKATRLT
                     GVEQALSGNIDAASIDAAAEQIGDLEYAGDHFASSEYRAHLTKVYVKRALKRAAGV"
     gene            complement(928..3312)
                     /locus_tag="VFV93_14625"
     CDS             complement(928..3312)
                     /locus_tag="VFV93_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5166636.1"
                     /translation="MVATEERQRLIGQRMKRKEDPRFITGRGRYVEDIVLPRMLHAVV
                     LRPPHAHAKINSIDTAKAATMPGVVAVFTGNDIELPGLPFAYPAAGVDNNVNTNTILA
                     KDEVHWAGDGVAVVVAETVSQAYDALDAIDVDYTPLPAVTDAEQATLPGAPQLHENAP
                     NNVAFDWTCGNKEGADEALANAEVTVRGRIRNQRLIATPMEMRGAICDYNAGTDEFTW
                     YVNSQAPHVHRLLFAAFVFGVPEQNIRVVAPDIGGGFGAKIFVYPEQLLVGWLSRKLN
                     RPVKWIERRQESHLTTQQGRDHVTDFEVAANRDGRVTGIRVKTWANLGGYLSTIAAGI
                     PTTLYGRMVAGVYDIPNIFVNVLGVYTNTALVDAYRGAGRPEATYLIERIMDHVAQAI
                     DMDPVDVRRKNFIASDAFPFDTGLGMLPYDSGDYPKALDKALEISDYAGLRQQQAGQR
                     ANGNGKYLGIGVSTYVETCGLAPSAWMASQGWGAALFESANIKIHLTGKVVVTTGSMP
                     QGQGHETSFSQVVADKLGVPFEDVQVKFGDTLGTPFGYGTYGSRSLAVGGEAIVRSAN
                     KIVDKAKKLAAHMLEVGEEDIEFGEGKAWVKGAPENAKTIQEIALAASVGYSLPEGME
                     PYLDETTYYDPPNATFPFGTHIAVVEVDAGTGAVELKRYVAVDDFGNLVNPMIVDGQL
                     HGGIVQGIGQALYEGAVYDDDGQLLTGTLMEYAIPHAEQFPKIETAFTVTPSPVNELG
                     VKGAGEAGTIASTPAVVNAVVNALAPLGITHIDMPLSPPRVWAAMQQARHEGGQ"
     gene            complement(3381..3872)
                     /locus_tag="VFV93_14630"
     CDS             complement(3381..3872)
                     /locus_tag="VFV93_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5166637.1"
                     /translation="MAHKVSLNVNGQQREGEVEARTLLVHFIRENLGLTGTHVGCDTS
                     QCGACTVIMDGLAVKSCTVLAVQADGAEVLTVEGLAKDGELHPIQEGFWEEHGLQCGF
                     CTPGMIMSSYDLLQRNPNPTEAEIRHAIDGNLCRCTGYQHIVKSIQYAANKMSGAAAS
                     GDD"
BASE COUNT          693 a         1305 c         1402 g          775 t
ORIGIN      
        1 gcagggcgag gctgcggcca tcgcccctac gcgggaagcg gaaacaatca acctcggccg
       61 tgaatggccg aggttgattg tttggctgtt cgatggagtg gttagacgcc agccgcccgc
      121 ttgagcgccc gcttgacgta gaccttggtg aggtgggcgc ggtactcaga cgaggcgaag
      181 tggtcgccgg cgtactcgag atcgccgatc tgctcagcag cggcgtcgat gctcgcagcg
      241 tcgatgttgc cggagagtgc ctgctcgaca ccggtgaggc gcgtcgcctt gtatgtcgcg
      301 ccggtgaggg caaccttgac accgccatcg ccaacgacgg cagcaacgcc gacgatcgcg
      361 tagccagacg ccggatgcgc gaacttctcg taggccgact tcggcgcggc ggccgggagc
      421 ttgatgctgg tcagcacctc gtccggctcc agcgccgtcg tcaggatgtc gacgaagaag
      481 tcgtcggcgg cgatggtccg ttcaccattg ggaccgacag cggtgaactc tgcgccgagc
      541 gccagcaggg cggccgggac gtcggcggcc gggtcagcgt gggcagccgc gccaccgatc
      601 gtgcctcggt tgcggacctg gacatcacct actgaggcga tgcattcgtg caggaccgga
      661 tagctggcgt tgagctgggc attgccgagc aggtcgttgt aagtgacacc ggcgccgatc
      721 gtcgcgccgc cgttgacgga gacgctcttg agctcggcca ccttgctgat atcgaccagt
      781 agcgacggcg aggccagccg cagcttcatg accggcagca acgagtgacc gccggcgatg
      841 agcttggcgt cgtcgttgcc cgagagcgcc tggatggctt cctggaccga gctgacctgc
      901 tggtactcaa atggacgtgg gtacacgtta ctgccctccc tcatgtctgg cttgctgcat
      961 cgccgcccaa acgcgcggcg ggctcaacgg catatcgatg tgggtaattc ccagtggcgc
     1021 cagcgcgttg acaacggcgt tgacgacggc gggcgtcgaa gcgatcgtcc cggcctcgcc
     1081 ggcgcctttg acgcccagct cgttgaccgg cgacggggtc acggtaaagg cggtctcgat
     1141 cttcgggaac tgttcggcgt gcgggatagc gtactccatc agcgtgcccg tcaggagctg
     1201 gccgtcgtcg tcgtagacag cgccctcata gagggcctgg ccgatgccct ggacgatccc
     1261 gccgtgcaac tgaccatcga cgatcatcgg gttgacgagg ttgccgaagt catcgacggc
     1321 gacgtagcgc ttgagctcga cggcgccggt accggcatcg acctcgacga cggcgatgtg
     1381 ggtcccgaac gggaaggtcg cattcggcgg gtcgtaatag gtcgtctcgt cgagatacgg
     1441 ctccatcccc tcgggaagcg aatagccgac cgaggcggcc agcgcgatct cctggatcgt
     1501 cttggcgttc tccggcgcac ccttgaccca ggccttgccc tcgccgaatt cgatgtcctc
     1561 ctcgccgacc tcgagcatat gggcggccag cttcttggcc ttgtcgacga tcttgttggc
     1621 gctacggacg atcgcctcgc cgccaacggc cagcgagcgc gagccgtagg tgccgtatcc
     1681 gaagggggtc ccgagcgtgt cgccaaactt gacctggaca tcctcgaacg ggacaccgag
     1741 cttgtcggcg acaacctgtg agaagctggt ctcgtggccc tgcccctgtg gcatcgagcc
     1801 ggtcgtgacg acgaccttgc cggtcaggtg gatcttgatg ttagcgctct cgaagagagc
     1861 cgcgccccaa ccctggctcg ccatccaggc ggacggcgcc agaccgcagg tctcgacata
     1921 ggtagagacg ccgatgccga ggtacttgcc gttgccattg gcacgctggc cggcctgctg
     1981 ctgccggagg ccggcgtaat cgctgatctc tagcgccttg tcgagcgcct tcgggtagtc
     2041 gcccgagtcg tagggcagca ttcccaggcc ggtatcgaac gggaaggcgt cggaggcgat
     2101 gaagttcttg cggcgcacat cgaccgggtc catatcgatc gcctgggcga cgtggtccat
     2161 gattcgctcg atcaggtagg tcgcctccgg gcggcccgcc ccgcgatagg catcaacgag
     2221 ggccgtgttg gtgtagacac caagcacgtt gacgaagatg ttggggatgt cgtaaacacc
     2281 ggcgaccatc cggccgtaga gcgtagtcgg gatgccggcc gcgatcgtcg agaggtagcc
     2341 accgaggttg gcccaggtct tgacacggat accggtgacc cggccgtcgc gattggcggc
     2401 aacctcgaag tctgtcacgt ggtcgcggcc ctgctgggtg gtcaggtggc tctcctggcg
     2461 gcgctcgatc cacttgaccg gtcggttgag cttgcgcgac agccagccga ccagcagctg
     2521 ctccgggtag acgaagatct tggcgccgaa gccgccgccg atgtcgggcg cgacgacgcg
     2581 gatgttctgc tccggaacac cgaagacgaa ggcggcgaac agcaggcgat gcacgtgcgg
     2641 cgcctgcgag ttcacatacc aggtgaactc atcggtgccg gcgttgtagt cgcagatcgc
     2701 gccgcgcatc tccatcggcg tcgcgatcaa ccgctggttg cggatcctcc cccggacggt
     2761 aacttcagcg ttcgcaagcg cttcatcggc gccctccttg ttgccgcagg tccagtcgaa
     2821 ggcgacgttg ttcggcgcat tctcgtgcag ttgcggcgcg ccaggcagtg tggcctgttc
     2881 ggcatcggtg acggccggca gcggcgtgta gtcgacgtcg atcgcgtcga gggcgtcgta
     2941 cgcctgcgac accgtctcgg cgacgacgac ggcgacgccg tccccggccc agtggacctc
     3001 atccttagcg aggatggtgt tggtgttgac gttgttgtcg acgccggccg ccgggtaggc
     3061 gaacggcagg cccggtagct cgatgtcgtt gccggtgaag acggcaacga cgccgggcat
     3121 cgtggctgcc ttcgcggtgt cgatgctgtt gatcttcgcg tgggcatgcg gtggccgcag
     3181 gacgacggca tgcagcatcc gtggcaggac gatgtcctcc acgtagcgcc cacggccagt
     3241 gatgaagcgc gggtcctcct tgcgcttcat gcgctggccg atcagccgct gtcgctcttc
     3301 ggttgctacc atgatcgcct cctaactcgt gatgcgtgat gcgtaatgcg tgacgcgtga
     3361 cgcgaaccga gttacggtga ctaatcgtcc cccgaagcag ccgcgccgga catcttgttg
     3421 gcggcgtact ggatcgactt gacgatgtgc tggtagccgg tgcagcggca gaggttgccg
     3481 tcgatcgcgt ggcggatctc cgcctcggtc ggattcggat tgcgctgcaa caggtcgtac
     3541 gacgacatga tcatgccggg tgtgcagaag ccgcactgca gaccgtgctc ttcccagaac
     3601 ccttcctgaa tcgggtgaag ctcgccatcc ttggccagcc cctcgacggt caatacctcg
     3661 gcgccgtccg cctgaacagc caggacggtg caggacttga cggccaggcc gtccatgatg
     3721 accgtgcagg cgccgcactg gctggtgtcg cagccgacgt gcgtgccggt cagaccgagg
     3781 ttctcgcgga tgaagtggac gagcagcgtc ctcgcctcga cctcaccctc gcgctgctgg
     3841 ccgttgacat tcaatgagac cttgtgcgcc atcttccctc cttcaacgag gtatccaagg
     3901 ctttcccaga cccgtcttca acgcgcccca ggctcatcgc ggacgtctcc ggtactctcc
     3961 cggcgtcgca cgcgacacgc gggttttcgg gagaacgcgg cgcacaagcg ccatgatccg
     4021 ggacgcgacg gcatagtagc acggacgtgg atgtggtcaa gcgtattcga tcaatatgga
     4081 cgatgtgccc tcgcggggtg tatggttata cgtatggtga tgcagaaaac gactgtctac
     4141 attcctcacg aaatgaaagc tcaactggag cagtt
//
LOCUS       DASUGJ010000639         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_522852, whole genome shotgun sequence.
ACCESSION   DASUGJ010000639 DASUGJ010000000
VERSION     DASUGJ010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522852"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /gene="zwf"
                     /locus_tag="VFV93_14635"
     CDS             complement(<1..1128)
                     /gene="zwf"
                     /locus_tag="VFV93_14635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5166638.1"
                     /translation="MSDSPVITASGGTLTAERARHKPASKVVNPLRIGLQTERNPQAC
                     AMVIFGVSGDLTSRKLMPALYDLYVSVPLPPGFSVVGISRRDWSDDDFRNEMRDAVRE
                     NATRPFTDDAWDSFARGLSYVRGDFDNAELYGRLREQLETVDSERGTNGNHLYYLATP
                     PSFYEEISAGLGNAGLVKRQEIYSNPRANGWDRIVIEKPFGHDLASAQHLNAAINAVF
                     AEQQIYRIDHYLGKETVQNVLAFRFANILFEPVWNRHYVDQIQVTVAESLGIENRATY
                     YEEAGALRDMVQSHMLQLLSVVAMEPPAFFNGDALRDEKVKVLRSVVPPDGDRVAKDV
                     VRGQYTTGYVAGTEVVGYRDEEGVNTESRTETFVGLKLEIDN"
     gene            complement(1192..>2106)
                     /gene="gndA"
                     /locus_tag="VFV93_14640"
     CDS             complement(1192..>2106)
                     /gene="gndA"
                     /locus_tag="VFV93_14640"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX5166639.1"
                     /translation="GAGHFVKMVHNGIEYGDMQLIAETYDIMKRALGMSTSEMADIFG
                     RWNQGKLSSFLIEITAKVLGTKDAITGEPLVEKILDAAEQKGTGRWTSQNALDLGTPI
                     PVIDAAVFARSISSFKARRLGASKILTGPGTAMEIADAEKQRLIGVLEDALYVAKISS
                     YAQGMALLRAASDAYSYGLNLSELARIWTGGCIIRAKLLEPIMTAYRNNQALDNLLLA
                     PEFVDTVNSATNACREAVVLARMHGLPCPAMSAALDYIDSYRTERLPANLIQAQRDYF
                     GAHTYKRIDTTGVFHANWDTGEAEQIQG"
BASE COUNT          352 a          637 c          710 g          407 t
ORIGIN      
        1 gttgtcgatc tccagtttga ggcctacgaa ggtctccgtg cgcgattcgg tgttgacgcc
       61 ttcttcgtcc cggtatccga cgacttcagt gccggcgaca tagccggtcg tgtactgacc
      121 gcggacgaca tccttggcga cgcggtcacc atcgggcggg acgaccgagc gcagcacctt
      181 gaccttctca tcgcgaagtg cgtcgccgtt gaagaaggcg ggcggctcca tcgcgacgac
      241 cgagaggagc tggagcatgt ggctctggac catgtcccgc agggcgccgg cctcttcgta
      301 ataggtggcg cggttctcga taccgagcga ctcagccacc gtcacctgta tctgatcgac
      361 ataatgtcga ttccagaccg gctcgaagag gatgttggcg aagcggaagg cgaggacgtt
      421 ctggaccgtc tccttgccga gatagtgatc gatccggtaa atctgctgct cggcgaagac
      481 ggcgttgatc gcggcattga ggtgctgcgc actggccagg tcgtggccaa acggcttctc
      541 gatcacgatc cgatcccagc cgttggcgcg cggattggag tagatctcct ggcgcttgac
      601 gaggccggcg ttgccgagtc cagccgagat ctcctcgtag aagctcggcg gggtcgcaag
      661 gtaatagaga tggttgccgt tggtgccgcg ctcggagtcg acggtctcga gctgctcgcg
      721 gaggcggcca tagagctcgg cgttgtcgaa gtcgccgcgc acgtaggaga gaccgcgcgc
      781 gaacgagtcc cacgcgtcgt ccgtgaacgg gcgcgtggcg ttttcgcgga cggcgtcgcg
      841 catctcgttg cggaaatcgt cgtcgctcca gtcgcgccgc gagatgccga cgactgagaa
      901 gccgggcggc agtgggaccg agacgtagag gtcgtagagg gccggcatca gcttgcgcga
      961 cgtgagatcg ccggacacgc cgaagatgac catcgcgcag gcctgcggat tgcgctcggt
     1021 ttgcaggccg atccgcagcg ggttgacgac cttgcttgcc ggtttatgcc gggcgcgctc
     1081 tgcggtgagc gttccaccgc tggcggtgat gaccggggag tcggacatga gagactccgt
     1141 ttcctgcact cgtcggcgcc gccgggtgct ggttgcgccc attaccagta attacccctg
     1201 gatctgctcc gcctcgcccg tgtcccaatt ggcatggaac acgccggtgg tgtcgatccg
     1261 tttgtacgta tgcgctccga agtagtcccg ctgcgcctgg atcaggttgg ccgggagccg
     1321 ctcggtgcgg taactgtcga tgtaatccaa cgcggcgctc attgccggac aagggagccc
     1381 gtgcatccgg gccaggacga ctgcttcgcg gcaggcgttc gtcgcagaat tgacagtatc
     1441 cacgaattcg ggcgccagga gcaggttgtc gagcgcctgg ttgttccggt aggcggtcat
     1501 gatcggctcg agcagcttcg cccggatgat gcagccaccg gtccagatgc gcgccagctc
     1561 cgacaggttc agcccgtacg agtaggcatc cgacgccgcc cgcagtaggg ccatgccctg
     1621 cgcgtacgac gaaatcttgg cgacgtagag ggcgtcttcg aggacgccga tcaagcgctg
     1681 cttctcggcg tcggcgatct ccatggcggt gccgggaccg gtcaggatct tgctcgcccc
     1741 aagccggcgc gccttgaagg aggagatcga gcgggcgaag accgccgcgt cgatgaccgg
     1801 gatcggcgta ccaagatcga gcgcgttctg gctcgtccag cgaccggtgc ccttctgctc
     1861 ggcggcatcg aggatcttct cgaccagcgg ctcaccggtg atcgcgtcct tcgtgccgag
     1921 gaccttggcg gtgatctcga tcagaaacga ggagagcttg ccctgattcc agcgaccgaa
     1981 gatgtccgcc atctcggatg tactcatccc gagggcccgc ttcatgatgt cgtaggtctc
     2041 ggcaatcaac tgcatgtcgc cgtattcgat gccgttgtgg accatcttga cgaagtgtcc
     2101 ggcacc
//
LOCUS       DASUGJ010000640         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1772880, whole genome shotgun sequence.
ACCESSION   DASUGJ010000640 DASUGJ010000000
VERSION     DASUGJ010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772880"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFV93_14645"
     CDS             <1..235
                     /locus_tag="VFV93_14645"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX5166640.1"
                     /translation="IGALSGSMRDVGVDDDFIKQIRNDVTEGTSALFLLTANAVPEKL
                     AEELKGRNLNFKIIASNLTKEQEDKLMELFSEE"
     gene            371..2065
                     /locus_tag="VFV93_14650"
     CDS             371..2065
                     /locus_tag="VFV93_14650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166641.1"
                     /translation="MNANDDATQYTSWLAMVIILMAQMQMGFNVNALVISIGAIVDDL
                     DTAATTVGTALVIYSLAVAGLVMLGAKVGSMIGSRLAFQIGVAAHGASMLWMALSPNA
                     GMMIAAQAVAGAAAALAVPALVVLIAANYTGKQQEQALGLLAAAVPASGVLAFLIVGV
                     LTELISWRWTFAALAIVSVAVLFLSVRLTPLERQAGVRIDWVGAILAASAITLISLGF
                     NSVNAWGLLVAGNAAPFEVLGLSPVPIFVFVGIVLIQAFFAWMRRQRSLNKPQLFEME
                     VLDSREEIHASISLLLIGALAAGVNFLIPLYVQIVQGRTTLQTAVAIVPYALAVLTSA
                     TFVVRLYSRYTPRQIGRVAFIAVSVGLLLLAYAIQNDWGTPTVIVSLVVIGLGEGSLL
                     TLLFNVLVSASPKDLAGHVGALRGTINNLATGLGTAIASVLAVASLGLLLAISFAESP
                     AIPDEIHDHIELDDVDFVSNDELEEALEGSALAPEVAQEVVRLNSEARLTALKISFQV
                     LAGMALVGIFPAGRLPAYVPGEVPSKPQRRTRQPILHRLARPTRRIVRSKRKTIFR"
     gene            2084..>2814
                     /locus_tag="VFV93_14655"
     CDS             2084..>2814
                     /locus_tag="VFV93_14655"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5166642.1"
                     /translation="MAEASDRARQYSRNNSDRFVDELDALLRIPSLSGDPAHAGDVRR
                     AAKWLADHLRTIGAGNVAVMETAGHPIAYGEWLGAGPDKPTVLVYGHYDVVPASIEDG
                     WDTEPFDPVIKDGKIYARGATDDKGQLMIHVNAFESYMKTGDGPPVNLKFLLEGEEEV
                     SSPNLRPFLESHLDLLQADVCVISDSSMPTIDEPAILHSLRGMTYVEIEVEGPHDDLH
                     SGFYGGAVHNPALALVEILSKLHHP"
BASE COUNT          493 a          901 c          900 g          520 t
ORIGIN      
        1 catcggagcg ctttccggct cgatgcggga tgtcggcgtc gatgacgact tcatcaagca
       61 gatccgaaac gacgtgaccg agggcacgtc tgcgctgttc ctgctgaccg cgaacgcagt
      121 gccggagaag ctggctgagg aactgaaggg gcgcaacctc aacttcaaga tcatcgccag
      181 caatctcacc aaggaacagg aagacaagct catggagctc ttcagcgagg agtaatcccc
      241 gcggccggca cgaacctggg ggacggggcg agtcgcttgt gccaacggac cggcggcgac
      301 ccgctccctc atgttcctgg actggcatcg actccggctc gcgaacgaag gttggctgat
      361 cggatctctg gtgaacgcga acgacgacgc aactcaatac accagctggc tggcgatggt
      421 tatcatcctg atggcgcaga tgcagatggg gttcaacgtc aacgcgctgg tcatctcgat
      481 cggcgcgatt gtcgacgacc tggacacggc cgcgacgacg gtcgggaccg cgctggtcat
      541 ctactcgctg gccgttgccg gtctggtcat gctcggcgcc aaagtcggca gcatgatcgg
      601 ctcgcggctg gcattccaga tcggtgtggc ggcccatggc gcctcgatgc tctggatggc
      661 gctaagcccg aacgcgggga tgatgatcgc ggcccaggct gtcgccggtg cggcggcggc
      721 gctggcggtt ccggcgctgg tcgtcctgat cgccgccaat tacaccggca agcagcagga
      781 acaggcgctc gggttgctgg cggctgccgt gccggcgtct ggcgtgctgg cgttcctgat
      841 cgtcggcgtc cttaccgagc tgatcagctg gcgctggacg ttcgcggcgc tcgccatcgt
      901 ttcagttgcc gtccttttcc tgagcgtccg gctgacgccg cttgagcggc aggccggcgt
      961 ccggatcgac tgggttggcg cgatcctggc cgcatcggcc atcacgctca tcagcctcgg
     1021 cttcaacagt gtcaacgcct ggggactgct ggttgccggc aacgcagcac cattcgaggt
     1081 gctcggcctc tcgccggtac caatcttcgt cttcgtcggt atcgtcctca tccaggcctt
     1141 cttcgcatgg atgcgccggc agcgttcgct gaacaagccg caactgttcg agatggaagt
     1201 gctcgactct cgcgaagaga tccacgcctc gatttcgctg ttgctgatcg gcgcgctggc
     1261 cgccggtgtg aacttcctga tcccactcta cgtccagatc gtccagggcc ggacgacgct
     1321 gcaaaccgcc gtcgcgatcg ttccctacgc actggctgtg ctgacatcgg cgaccttcgt
     1381 cgttcgcctg tacagccggt acaccccacg ccagatcggc cgggtcgcgt tcatcgcggt
     1441 gtcggtcggg ctgctgctgc tcgcctacgc gattcagaac gactggggaa caccgaccgt
     1501 tatcgtcagc ctggtcgtga tcgggcttgg cgaaggctca ttgctgacgc tgctgttcaa
     1561 tgtgctcgtc tcggcgtcgc cgaaggactt ggccggccat gtcggcgcgt tgcgcggcac
     1621 gatcaataat ctcgcgaccg gcctggggac ggcgatcgcc agcgttctgg ccgtcgcatc
     1681 actcggactc ctgctcgcga tcagcttcgc ggagagtccg gccattcccg atgagatcca
     1741 cgaccacatc gagctcgacg atgtcgactt cgtcagcaac gacgaactgg aagaagccct
     1801 cgaaggctcg gcgctggcgc cggaagttgc ccaggaggtc gtccggctca actcggaagc
     1861 acggctgacc gcgctcaaga tctcgttcca ggtgctggca gggatggcat tggtcggcat
     1921 ctttccagcc ggacggttgc cggcctacgt gccgggcgag gtgccgtcga aaccgcagcg
     1981 gcggacacgg caaccgatcc tgcacaggct cgcccgtcca acgcggcgca tcgtccggtc
     2041 gaaacgcaag acgatctttc gttgaggaaa ggggaggcgt tctatggcag aggcaagtga
     2101 ccgtgcgcgt cagtactcac gcaacaacag cgaccggttt gtcgacgagc tcgatgcgtt
     2161 actgcgcatc cccagcctga gtggagaccc tgctcacgcc ggcgatgtcc ggcgcgcggc
     2221 aaaatggctg gccgaccacc tgaggacgat tggcgccggg aacgtggccg tcatggagac
     2281 ggccgggcat ccgatcgcct acggcgagtg gcttggcgcc ggccccgaca agccgacggt
     2341 gctggtctac ggacactacg atgtcgtacc ggcgtcgatc gaggacggct gggataccga
     2401 gccgttcgat ccggtcatca aggacggcaa gatctacgcg cgcggcgcga ccgacgacaa
     2461 gggccagttg atgatccacg tcaacgcctt cgagtcgtat atgaagaccg gagacggccc
     2521 tcccgtcaac ctgaagttcc tgctcgaagg ggaagaagaa gtctcgtctc cgaacctgcg
     2581 gccattcctc gaatcgcatc tcgacctgtt gcaggctgac gtctgcgtca tcagcgactc
     2641 gtcaatgcca accatcgacg agccggccat tctccacagc ctgcgcggca tgacctacgt
     2701 cgagatcgag gtcgaaggcc cgcacgacga tctgcatagc ggcttctacg gcggcgccgt
     2761 ccacaacccg gctctcgcgt tggtcgagat cctgtcgaag ctgcaccatc cgga
//
LOCUS       DASUGJ010000641         2709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_523043, whole genome shotgun sequence.
ACCESSION   DASUGJ010000641 DASUGJ010000000
VERSION     DASUGJ010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523043"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /gene="ffs"
                     /locus_tag="VFV93_14660"
     ncRNA           complement(<1..125)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFV93_14660"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            201..1427
                     /locus_tag="VFV93_14665"
     CDS             201..1427
                     /locus_tag="VFV93_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166643.1"
                     /translation="MPHPNRHLRLLAALLLSLLALASFVFPAAAQDSGLVTGTVTNQT
                     TGAPVAGIEVTLSKFADQTGQNSEDTTATTGDDGTFRFEGLDTSDGLAYAVSTRYEDV
                     LYGTTMILLSQQPQQQADITVYETTTDQNSVTVPTRGIIISGVDPESGVLAMTDAYTF
                     NVDGDLTVVEGGDGYSVRFPVPENVDNITPRQGFNFGTARVEETDVLVTTPLHPGETS
                     AGLDYEFHYTGDSLDVPLSAAYPTKSLQILVPADVNDEEILVSAAGSPLLDNGIVPIN
                     GRDFHVWSASGLQPGATMTLSITGLPEPPTAHTLNTIEPAILAGLTLLAASAVTGWLV
                     VSRGLHKPRPVVLAPAASAPLDQRRELLSSELRDLEAAWQAGELEEPAYRASRRAILE
                     DLRSISRQYRGLGDDE"
     gene            1492..>2709
                     /locus_tag="VFV93_14670"
     CDS             1492..>2709
                     /locus_tag="VFV93_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166644.1"
                     /translation="MAHDQARRITADDIYRFKLISEPSFSPDNERISYVVTTIEREKN
                     DYRSSINVANADGSGSRRITQADAKDSLPRWSPDGKHLAFLSNRDEKSQVWLIRADGG
                     EAWQVTSLSEGVNSYAWSPDGASFVVVSKSTEESEAAKDKEEKDEEKSDVRHITRIRY
                     KADGEGFLDFKPKHLWLVPAFGGDAQQLTSADISDGDPVWSPNGREIAFVSNRSEGRE
                     MNNVSQVWSIVATGKEERAILGGDNAAFGQPSWSPDGSQLAAIGNWDPASGGAKNDNL
                     WVVPAGGGEARNLSGDFDRSIGDSAMSDVYAGSSNRPLWTPDGASLLTLVSDSGSTHI
                     YRVSLDGGDITRITGGERRVSGVSLASDGKAIAYVAGTATNPGDLYVANIDGSDERQL
                     TNLNQDFLGSLTLS"
BASE COUNT          533 a          973 c          799 g          404 t
ORIGIN      
        1 cgcgggacct gaccttcatc accacttggc cgggcagtcc caaaggatcg gacctcagaa
       61 gggaattccg tcccgctaaa gcggattgcg ggtgcagggc accgctagct ccccacctag
      121 cacgaccatc cgaagtatac atccctgttg tcacagcgtc acacggtaca attgcaccga
      181 tgaacgcgaa tgaccgcccg ttgccacacc caaatcgaca tctgaggctg ctcgccgcgc
      241 tgctgctttc gctgttggcg ctggcctcgt tcgtcttccc cgcagccgcc caggacagcg
      301 gcctcgtcac cggcaccgtc acgaatcaga cgaccggcgc gccggttgcc gggatcgagg
      361 tgaccctctc gaagttcgcc gaccagaccg gccagaacag cgaagacaca accgccacga
      421 ccggcgacga cggcaccttt cgcttcgagg gcctcgacac gtccgatggg ctggcctacg
      481 ccgtctcgac ccgctacgaa gatgtcctct acggcacgac gatgatcctg ctgagtcagc
      541 aaccgcaaca gcaggccgac atcaccgtct acgagacgac gaccgaccag aacagcgtga
      601 cagtcccgac ccgcggcatc atcatcagcg gcgtcgaccc ggagtccggc gtcctcgcga
      661 tgaccgatgc ctacaccttc aacgttgacg gcgatctcac cgttgtcgag ggcggcgacg
      721 gctacagtgt ccgcttcccg gttcctgaga acgtcgacaa catcacccca cggcagggat
      781 tcaactttgg aaccgcccgc gtcgaggaga ccgatgtcct cgtgacaacg cccctccacc
      841 cgggcgagac cagtgccggc ctcgactacg agtttcacta caccggcgac tcgttggacg
      901 tgccgctctc agccgcctac ccgaccaaat cgctgcagat cctcgttccg gctgatgtca
      961 acgacgagga gatcctcgtc agcgccgcgg gcagtccgct actcgacaat ggcatcgtcc
     1021 cgatcaacgg ccgagacttc cacgtctgga gcgccagcgg cctccagccc ggcgcgacga
     1081 tgacgctcag catcacagga ctgcccgagc cgccgaccgc ccacacgctg aacacaatcg
     1141 aaccggccat cctcgccgga ctcacactac tggcggcatc ggccgtgacc ggttggctcg
     1201 tcgtctcgcg tggcctgcac aagccgcgac cggttgtcct cgctccggct gcatctgccc
     1261 cgctcgacca gcgccgcgaa ctcctgtcaa gcgaactacg cgacctcgaa gccgcctggc
     1321 aggctggcga gctcgaagaa ccggcctacc gggcatcacg cagagctatc ctggaggacc
     1381 tgcgtagcat ctcgcgccag taccgcggcc tcggcgacga cgagtagcgc gatgcgcgaa
     1441 gcgtgctacg ataccccgcc ggaacgccga acagggaggg gacacaaccg aatggcacac
     1501 gaccaggcac gacgaatcac cgccgacgac atctaccgtt tcaagctgat cagcgagccg
     1561 agcttctcgc ccgacaacga gcgcatcagt tacgtcgtca cgacgatcga gcgcgagaag
     1621 aacgactacc gctcgtcgat caatgtcgcc aatgccgacg gctccggtag ccgccgtatc
     1681 acccaggctg acgcgaagga ctcgctgccg cgctggtcgc cggacggcaa gcacctcgcc
     1741 tttctctcaa accgcgacga gaagtcacag gtctggctga tccgcgctga cggcggcgaa
     1801 gcctggcagg tcacgtccct ctccgagggc gtcaatagct atgcctggtc gccggacggc
     1861 gccagcttcg tcgtcgtctc caagtcgacc gaggaatcag aggccgccaa ggacaaggag
     1921 gagaaggacg aggagaagtc cgacgttcgc catatcacca ggatccgtta caaggcggac
     1981 ggtgaaggct tcctcgactt caagccgaag catctctggc tcgtgccggc cttcggtggc
     2041 gacgcgcagc agctcacctc ggccgacatc agcgacggcg atccggtctg gtcgccgaat
     2101 ggacgcgaga tcgccttcgt cagcaaccgc agcgagggcc gcgagatgaa caatgtctcg
     2161 caggtctggt cgatcgtcgc gaccggcaag gaggagcgcg cgatcctcgg cggtgacaac
     2221 gccgccttcg gccagccgag ctggtcgccc gacggcagcc agctggcggc gatcggcaac
     2281 tgggacccgg cgtccggcgg cgcaaagaac gacaacctct gggtcgtacc ggccggcggc
     2341 ggcgaagcgc gcaacctcag cggagacttc gatcgctcga ttggcgactc agccatgagc
     2401 gatgtctacg ccggctcgtc caaccgtccg ctctggacgc cggacggcgc cagcctcctg
     2461 acgctcgtca gcgactcggg ctcgacccat atctaccgtg tctcgctcga tggcggcgac
     2521 atcacccgca tcaccggtgg cgagcggcgc gtctcgggcg tctcgctcgc cagcgacggc
     2581 aaggccatcg cctacgtcgc cggcacggca accaacccag gtgacctcta cgtcgccaac
     2641 atcgacggca gcgacgagcg ccagctgacg aacctgaacc aggacttcct cggcagcctc
     2701 acgctctcc
//
LOCUS       DASUGJ010000642         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2050906, whole genome shotgun sequence.
ACCESSION   DASUGJ010000642 DASUGJ010000000
VERSION     DASUGJ010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050906"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1415
                     /locus_tag="VFV93_14675"
     CDS             129..1415
                     /locus_tag="VFV93_14675"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166645.1"
                     /translation="MRSYIRGVQGYGRDIKLFLLYNLLSNIGIGVFQLLFNLYLIELD
                     YDESFIGQFQSISTLSMAGVALSIGLLVNRFGVWRTVTTGLVFFLITSVLASIITNQL
                     ALLLMAGLSGAGTAFLFVPTMPFIVELTSRNERHGVAALAFSLNSLSVTVGSLIGGFG
                     ARGIGAALDLDVPSAEAYRYTLIAGLLLAGFAILPLLAMTSERKRALPEDEQSVGLPA
                     THAVAETPQKIRRHMIVFIAIGGLMSLGAGAVFPFYNVFLETRGASSGQIGIIYAVAG
                     LTAAVVGLFSPWVAHRFGSLKATVIVRLSPVPCYMLLMATPILPVAIVAHILRTTSIN
                     MAWPIDSTYISEILPAKSRASVFSFRSGAWNLGWSLSSLIAGYAIVRYGYNVSFATYM
                     IFMTLAMGLFYIYFTRAAAPEPRPAPETSEPVAATQ"
     gene            complement(1421..1795)
                     /locus_tag="VFV93_14680"
     CDS             complement(1421..1795)
                     /locus_tag="VFV93_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5166646.1"
                     /translation="MSESGERTRIDEFREQRQRHNERIFEVDHLGIKRFFRLDSAAYE
                     QGALDPKTKELLGLVASTVLRCNDCIDYHLDQCVDVGWTDDELYDALNVALIVGGSIV
                     IPHLRHAVESIDLIRAGSAAGE"
     gene            complement(1895..2275)
                     /locus_tag="VFV93_14685"
     CDS             complement(1895..2275)
                     /locus_tag="VFV93_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166647.1"
                     /translation="MQFFMKPSRTDPASEIDEVTGQRVIKRLRHEGRKLIEVFVLEHD
                     GSIDRYQERTRRKLGSNAVASRTWLCETAQEGGWGNGRYIPEPADAIAEADPAPLAVV
                     LVLAGAEGEPESTVEQLRSLPSAT"
     gene            complement(2358..>2587)
                     /locus_tag="VFV93_14690"
     CDS             complement(2358..>2587)
                     /locus_tag="VFV93_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166648.1"
                     /translation="AAYNVSGEYAMVRAAGLAGWIDERRVTMETLTSIRRAGANQIIT
                     YHAKAAARWLMEENGWASAAVPSPAAVPALD"
BASE COUNT          398 a          881 c          793 g          515 t
ORIGIN      
        1 ggctcaggac gacagtgccg tttgaacccg tcacagagtc gtaccaaaac gcactctgcg
       61 catcgttctg ggggcgtcgc ccatctataa tccctccggc ttgtcttccg gagtgaatcg
      121 agtgaggcgt gcgctcctat attcgcggtg tccagggcta cggccgtgac atcaagctct
      181 tcctgctcta caacctgctg tctaacatcg gcatcggtgt tttccaactg ctcttcaatc
      241 tgtacctgat cgagctagac tacgacgagt cgttcatcgg acagttccag tcgatcagca
      301 cgctctcgat ggccggtgtt gcactgtcga tcggcctgct cgtcaatcgc ttcggcgtct
      361 ggcggacggt gacgaccggc ctcgtcttct ttctgattac ctcggtcctc gcgtcgatca
      421 tcaccaatca actcgcgctg ctcctgatgg cgggtctctc gggcgccggt acggccttcc
      481 tgttcgtgcc gacgatgccg ttcatcgtcg agctgaccag ccgcaatgag cggcacggcg
      541 ttgcggcgct cgccttctcg ctcaactcgc tgtcggtaac ggtcggcagc ctgatcggcg
      601 gctttggcgc gcgtggaatc ggcgcggccc tcgaccttga cgtgccgagc gcggaggcgt
      661 atcgctacac gctgatcgcc gggctgctgc tagccggatt cgcgattctg ccgttgctgg
      721 cgatgacgag tgagcgcaaa cgcgccctgc cggaagacga gcagtccgtc ggcctgccgg
      781 cgacgcacgc cgtcgccgag acgccgcaga agatccggcg gcacatgatc gtcttcatcg
      841 caattggcgg gctgatgagc ctcggcgcgg gggccgtctt cccgttctac aacgtcttcc
      901 tggagacgcg cggcgccagt tccggccaga tcggcatcat ctacgcggtg gccggactga
      961 cggcagcagt cgtcgggctg ttctcaccct gggtagcaca ccgcttcggc tcgttgaagg
     1021 cgaccgtcat cgtccgcctc tcgccggtgc catgctacat gctcttgatg gcgacaccga
     1081 tcctgccagt ggcaatcgtc gcccatatcc tacgcacgac atcaatcaac atggcctggc
     1141 cgatcgacag cacctacatc tccgagatcc tgccggccaa gtcgcgcgcc tcggtcttct
     1201 cgttccgctc cggcgcctgg aacctgggct ggtcactctc cagcctgatc gccggctacg
     1261 cgatcgtgcg ctacggctac aatgtctcgt tcgcgaccta catgatcttc atgacgctgg
     1321 cgatgggcct gttctacatc tacttcaccc gggccgccgc gccggaacct cgccctgcgc
     1381 ccgagacgtc cgagccggtg gcagccacgc agtaggcgca ttactcgcca gccgcggagc
     1441 ccgcccggat cagatcgatc gactcaacgg cgtgccgcag gtgcggaatc acgatgctgc
     1501 cgccgacgat cagcgcgaca ttcagcgcgt cgtagagctc gtcgtccgtc cagccgacat
     1561 cgacacactg gtcgaggtgg tagtcgatgc agtcgttgca gcgcagaacg gtcgaggcga
     1621 ccaggccgag cagttccttc gtcttcgggt cgagcgcgcc ctgctcgtag gcggcgctgt
     1681 cgagccggaa gaagcgcttg atgccgagat gatcgacctc gaagatacgc tcgttgtgtc
     1741 gctggcgttg ctcgcgaaac tcatcgatgc gggttcgctc gcctgattcg ctcactggtc
     1801 gatctcccat ccgtccggtt gaactcggtc catgcacaac gaagcccgca caggtcatgc
     1861 gggccgcgtc tcaggattca gcgtccggca accgtcacgt cgcggacggc aggctgcgca
     1921 gttgctcgac ggtcgattcc ggctcgccct cggcgccagc cagcaccagc accaccgcca
     1981 gcggggccgg gtcggcctcg gcaatcgcgt cggccggttc ggggatgtag cgcccgttgc
     2041 cccagccgcc ttcctgggcc gtctcacaga gccaggtccg tgacgcgacg gcgttcgagc
     2101 cgagcttgcg ccgggtccgc tcctggtagc gatcgatcga gccgtcgtgc tccagcacga
     2161 agacttcgat cagtttgcgg ccctcatggc gcagccgctt gatgactcgt tgaccggtca
     2221 cctcgtcaat ctcggacgcc ggatcggtcc gggatggttt catgaagaat tgcacacggt
     2281 cctcctgttg ccgcacgccg gccggcgtgg gcggtcttgc ttctgtcagc ctgattgtac
     2341 gccttcagac tccggcgtca gtcgagagcg ggcactgccg ccggtgacgg cacggccgcc
     2401 gaggcccaac cgttctcctc catcaaccag cgggcggcgg ccttggcgtg gtaggtgatg
     2461 atctggttcg cgccggcccg tcggatcgag gtcagcgtct ccatcgtcac gcgccgctcg
     2521 tcgatccagc cggccagacc ggctgcccgg accatcgcgt actcgccact gacgttgtag
     2581 gcggcaa
//
LOCUS       DASUGJ010000643         5481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1493922, whole genome shotgun sequence.
ACCESSION   DASUGJ010000643 DASUGJ010000000
VERSION     DASUGJ010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493922"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VFV93_14695"
     CDS             <1..886
                     /locus_tag="VFV93_14695"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5166649.1"
                     /translation="ELFMTDTAQYADIVLPATTQLEQVDLHKGYGHRFLQFNHPSIEP
                     LGEARSNWDTMRALAAELGYDDWWLRQEPIEVLEEIFEATKAYNPLLEGITIERLMAE
                     GTVPLTFDAVGPTPFADHRFPTPSGKVELYCEAMIAHGQDPLPRYTPPSEFADVERGS
                     KLVLMSPAAHHFVSSSLANVPKLMRLEQTPSIDINPADAAERGIAHGDDVIVENQRGW
                     CRLRAVVTDDVPPGVAISVKGQWPKLAPGGRNINYVTSDALADLGGQSTFHSNLVDVR
                     LATPADVESTREALVPAD"
     gene            complement(988..1329)
                     /locus_tag="VFV93_14700"
     CDS             complement(988..1329)
                     /locus_tag="VFV93_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166650.1"
                     /translation="MPTPTHSAIGDPLEAAIAFMEPYVFDIVERIDIDEFTTVEFIEA
                     MQLDPAARQAYLDTVEHWHEHDETAAKMVIHGQVIPTLLRRSGKVEWIGFAHGVADDY
                     AVPAWWRKVEQ"
     gene            complement(1406..3427)
                     /locus_tag="VFV93_14705"
     CDS             complement(1406..3427)
                     /locus_tag="VFV93_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5166651.1"
                     /translation="MPRDIPVANGRLLVTFDSRYRLRDVYFPHIGQENHTDGSPCTFG
                     VWTQDIDNPAERRFAWVHDDSWTRSLRYRDASLVTDVQLTNAQLGISLACSDAVDIDL
                     DAYLRAVVVHNDSPVAKRVRLFFHFDARLWGNVSGDTAFLDPDYRALVHYKGKRYLWL
                     SGCSESAAGLSSWAIGNKYIATSEGTWRDAEDGVLGENPVAQGSIDTVAALDVTVAPG
                     DDATVWFWLAAGFRFDDVRRLHEEIVERSPAMLIERTDRYWSFWSRKQSSQLSSVPKA
                     VADLYQQSLLILRTQIDDGGAILAANDSDIFQFGRDTYSYMWPRDGALVSIALDTAGY
                     SSLARQFYVFASELITRHGFFLHKYNPDGSAGSSWHPWATPSGELQFPIQEDETALVL
                     YGLDIHLRTTLDTELVRRLYDDFIAPATRFLLDYRDPVTGLPQPSYDLWEERRGVMAF
                     TVGSVWAGLHAGARFADLFADETLARDCRAAAGQMRDAVGTYLYDASLGRFLRRIVTD
                     AGGSVIERDPTLDSSLVGLSMFGMLAADDPRVVATMEQVERGLWVQTDIGGIARYTND
                     YYHQLTSDTITVPGNPWIICTLWLADWYIAKSASPADLERAMELIEWVVARALPSGTL
                     PEQVHPYSGQPLSVSPLTWSHATFIQTVRNFIKRQDQFDRSGWETFLTV"
     gene            3491..4162
                     /locus_tag="VFV93_14710"
     CDS             3491..4162
                     /locus_tag="VFV93_14710"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5166652.1"
                     /translation="MLNRVKVVATASGRREEGTRVGVSALEVQAGAVPAMNDISDRTL
                     LQGVRSGDERAFEALFERHYAGVFGVVIRIVGTPEEAEEVTQDTFLKLYRRPLADDDD
                     ANVRGWLYRVATNAAFNSVRSRRRRLGWLKRLAGRAERQLDDDPLDHVTRRDEAEIVR
                     DHLARLPERQRAALVLRSSGMSYAEVAGAIGVSPNSVGTILSRAERAFRKSYESTNGR
                     NGGSS"
     gene            4159..5337
                     /locus_tag="VFV93_14715"
     CDS             4159..5337
                     /locus_tag="VFV93_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166653.1"
                     /translation="MNLRRRRGCPTDGELRLALDSDGAVAEPLRQHLAGCADCSRRFA
                     EIAGAADLTKRTCTALGVGQPAPDVQLAYSRFRNSLAEQPARDISGGTRMGQLFAMRS
                     MRAASAVVAILAVVVAVTFTPMRTVADDFLNQFRVQKFAAVTIPMDALSPMQSLATAG
                     MSDADKQQFQDELKQLGAFDTTFDYDQDHLPQAMTLDEAEAQFGNIDEPDDLPDGFDN
                     APNAYLTEAGSASFDLDTAKAQQIIDALNLPIYSLPDPAQYPTLEFTANVPAAALLEY
                     QNADGKRIVVGQMESPTLDIPDGLDMDALREEILRFPGLPSDLVAQLRAIDDWEHTLI
                     IPIPEGATSENITINGEPGLLIEHELGSAVLWEKDGILHAVIGQVSADAVRDVADSTQ
                     "
BASE COUNT          979 a         1725 c         1850 g          927 t
ORIGIN      
        1 cgagctgttc atgaccgaca cggcccagta cgccgacatc gtcctgccgg cgacgacaca
       61 gctggagcag gtggacctgc acaagggcta cgggcatcga ttcctgcagt tcaaccaccc
      121 gtcgatcgag ccgcttggcg aggctcgcag caactgggac acgatgcggg cgctggccgc
      181 cgaactgggc tacgacgact ggtggctgcg gcaggagccg atcgaggttc ttgaggagat
      241 cttcgaagcg accaaggcat acaacccgct gctggagggc atcacgatcg agcgtctgat
      301 ggccgaaggc accgttccgc tgaccttcga tgccgtcggt ccgacgccgt ttgcggacca
      361 ccggttcccg accccgtccg gcaaggttga gctgtactgc gaggcgatga tcgcccacgg
      421 tcaggacccg ctgccgcgct acacgccgcc gagcgagttt gccgatgtag aacgcggctc
      481 aaagctggtg ctgatgtcgc cggccgccca ccacttcgtg tcgagcagcc tggcaaacgt
      541 gcccaagctg atgcggctgg agcagacgcc gtcgatcgat atcaatccgg ccgacgccgc
      601 cgagcggggc atcgcccacg gcgacgacgt catcgtcgag aaccagcgcg gctggtgccg
      661 gctgcgggcc gtcgtcaccg acgacgttcc gccaggcgtc gcgatctcgg tcaagggcca
      721 gtggccgaaa ctggcgcccg gcggacgcaa catcaactac gtgacatccg acgcgctggc
      781 cgacttgggc gggcagtcga ccttccacag caacctcgtc gatgtccggc tggcaacacc
      841 agctgatgtc gagtcgacgc gtgaggcgct ggttccggcg gattgatctc tgtcgtcctg
      901 agccgcagcg aagtacctct cctcgctcaa ccaccaatgc agcgaaggcg gggaggtcct
      961 ttgctgcggc tcaggacgac aggctggcta ttgctccacc ttccgccacc aggcggggac
     1021 ggcgtagtcg tcggcgacgc cgtgtgcgaa gccgatccac tcgaccttgc cgctgcggcg
     1081 aagcagggtc gggatcactt gtccgtggat gaccatcttg gcggcggttt cgtcgtgctc
     1141 gtgccagtgc tcgacggtgt cgagatacgc ctgccgtgcg gcgggatcga gctgcatcgc
     1201 ctcgatgaac tcaactgtcg tgaactcgtc gatgtcgatc cgctcgacga tgtcgaacac
     1261 gtacggctcc atgaacgcaa tcgccgcctc cagcgggtcg ccgatcgcgc tgtgggttgg
     1321 tgttggcatg aattcctcca acgggacgtg ggacgcggga cgcaggaggt gagtttggtt
     1381 tccttcgcgt cccgcgccac gaccgtcaca ccgtcaaaaa cgtctcccag cccgagcggt
     1441 cgaactggtc ctggcgtttg atgaagtttc gcacggtctg gatgaaggtc gcgtggctcc
     1501 aggtcagggg tgagacggag agcggctggc cgctgtaagg gtggacctgc tcgggcagcg
     1561 tgccgctcgg tagcgcgcgg gcgacgaccc attcgatcag ttccatcgcc cgttcgaggt
     1621 cggccggcga ggcggatttg gcgatgtacc agtcggccag ccagagggtg cagatgatcc
     1681 acgggttgcc ggggacggtg atggtgtcgg acgtgagctg gtggtagtag tcattggtgt
     1741 agcgggcgat gccgccgatg tcagtctgga cccagaggcc gcgctcgacc tgctccatcg
     1801 tcgcgacgac gcgcgggtcg tccgctgcca gcatcccgaa catcgacagt ccgaccaggc
     1861 tggagtcgag cgtcgggtcg cgctcgatga ccgagccgcc ggcatcggtg acgatccggc
     1921 gcaggaagcg gccaaggctg gcgtcgtaca ggtacgtacc gaccgcgtcg cgcatctgcc
     1981 cggcggccgc gcggcagtcg cgagccagcg tttcgtcggc gaacaggtcg gcgaaacgcg
     2041 ctccggcgtg tagcccggcc caaaccgagc cgacagtgaa cgccatcaca ccgcgccgtt
     2101 cttcccagag atcgtacgac ggttgcggca ggccggtgac agggtcgcga tagtcgagca
     2161 ggaagcgtgt ggctggcgcg atgaagtcgt cgtagaggcg gcggaccagt tcggtgtcga
     2221 gcgtcgtccg caggtggatg tcgaggccgt agaggaccag tgccgtctcg tcctcctgaa
     2281 ttgggaactg caattctccg ctcggtgtcg cccacggatg ccacgaggag ccggccgagc
     2341 cgtcggggtt gtacttgtgc aggaagaagc cgtgacgggt gatcagctcc gaggcgaaga
     2401 cgtagaactg acgagccagc gacgagtagc cggcggtgtc gagcgcgatt gagacgagcg
     2461 cgccgtcacg cggccacatg tacgaatacg tgtcacggcc gaactggaag atgtcggagt
     2521 cgttggcggc cagaatcgcg ccgccgtcgt cgatctgggt ccgcaggatc agcagcgatt
     2581 gctggtacag atccgcgacc gctttcggca cgctcgacag ctgcgacgat tgcttgcggg
     2641 accagaacga ccagtagcgg tccgtccgtt cgatcaacat cgccgggctg cgctcgacga
     2701 tctcctcgtg caaccgccgg acatcgtcga aacggaagcc ggccgccagc cagaaccaga
     2761 ccgtcgcatc gtcgcccggt gcgactgtga catccagcgc ggcaacggtg tcgatcgacc
     2821 cctgggcgac cgggttctcg ccgaggaccc cgtcctcggc gtcgcgccag gtgccttcag
     2881 aggttgcgat gtacttgttt ccgatcgccc acgacgagag gccggctgcc gattcgctac
     2941 agccggataa ccagaggtag cgtttcccct tgtagtggac gagtgcgcgg tagtcgggat
     3001 cgaggaacgc ggtgtcgccg gagacgttgc cccacagccg cgcgtcgaag tggaagaaga
     3061 gccgtacccg cttcgcgacc gggctgtcgt tatggacgac gaccgcgcgc aggtaggcgt
     3121 cgaggtcgat atcgaccgcg tcagagcagg ccagcgagat gccgagctgg gcgttggtga
     3181 gttggacatc ggtcacgagc gaggcgtcgc ggtagcgtag tgatcgcgtc cagctgtcgt
     3241 cgtggaccca ggcgaagcgg cgctcggcgg ggttgtcgat atcctgcgtc cagacgccga
     3301 aggtgcaggg actgccgtcg gtgtgattct cctggccgat gtgtgggaaa tagacatcgc
     3361 gtagccggta gcggctgtcg aatgtcacca gcagtcgccc atttgcgacc ggaatatcgc
     3421 gcggcatcgt tccctctcag acacagacca ccacttccaa tccaggattg tcacataccg
     3481 ggagctactg gtgttgaata gggtgaaggt cgtcgcgacg gcctccggac ggagagaaga
     3541 agggacgcga gtgggcgtca gcgcgctgga ggtacaggcg ggagcggttc ccgcgatgaa
     3601 cgatatttcc gaccggacgc tcctgcaagg cgttcggagc ggagatgagc gtgcattcga
     3661 ggctctcttc gagcgtcact acgcaggagt cttcggtgtc gttatccgga tcgttgggac
     3721 gccggaagag gcggaggaag tcactcagga cacgttcctg aagctctatc gccgcccact
     3781 ggccgacgat gacgacgcga atgtccgcgg ctggctctac cgggtcgcca ccaacgccgc
     3841 cttcaacagt gtccgatcgc ggcgtcgccg gctcggctgg ttgaagcggc tcgcaggccg
     3901 ggccgagcgc cagctggatg acgatccact cgatcacgtc acgcggcgtg acgaggccga
     3961 gatcgtgcgg gaccatctcg cgcggctccc ggagcggcaa cgggcagcgc tcgtgctacg
     4021 ctcgtcaggc atgtcgtacg ccgaagtcgc cggagcgatc ggcgtcagcc ccaactcggt
     4081 cgggacgatc ctgtcgcggg ccgagcgggc gtttcggaag tcgtatgaat cgacgaacgg
     4141 tcgaaacgga ggcagctcat gaatcttcgc cgtcgccgcg gctgccccac cgacggggag
     4201 ttgcggctcg ctctcgacag tgatggcgcg gtcgccgaac cactccggca gcaccttgcg
     4261 gggtgcgcgg attgctcacg gcgcttcgcc gagatcgccg gcgcggccga tctgaccaag
     4321 cgaacgtgta ccgcgctcgg cgttggccag ccggcgccgg acgttcagtt ggcatacagc
     4381 agattccgca acagcctggc cgaacagccg gccagggata tatcaggagg aacgagaatg
     4441 ggacagctct tcgcgatgcg ctcgatgcgc gcggcctcgg cggtcgttgc catcctggcg
     4501 gtcgtcgtcg ccgtcacgtt cacaccaatg cgaacggttg ccgacgattt cctgaaccag
     4561 ttcagggttc agaagttcgc ggccgtcacg atcccgatgg acgcgctctc gccgatgcag
     4621 tcgctggcga cggccgggat gtcggatgcc gacaagcagc agttccagga tgagctgaag
     4681 cagctcggtg cgttcgacac gacctttgac tacgaccagg atcatctgcc gcaggcgatg
     4741 acgctggacg aggccgaggc gcaattcggc aacatcgacg agccggatga cctgccggat
     4801 ggcttcgaca acgccccgaa cgcctacctg accgaggccg gctcggcgtc gttcgacctc
     4861 gatacagcca aggcacagca gatcatcgac gcgctcaacc tgccgatcta ctcgctgccg
     4921 gacccggccc agtacccgac gctggagttt acggccaacg tgccagccgc tgcgcttctc
     4981 gaataccaga acgcagatgg caagcggatt gtcgttggcc agatggaaag cccgacgctc
     5041 gacatccccg atgggctcga catggacgcg ctgcgcgagg aaatcctgcg cttcccgggc
     5101 ctgccgtccg acctcgtcgc ccaactgcgc gcgatcgacg actgggagca cacgctgatc
     5161 atcccgatcc cggaaggcgc gacgagtgag aacatcacga tcaatggcga gcccggtctg
     5221 ctgatcgagc acgagctcgg cagcgcggtc ctctgggaga aggacggtat cctgcacgcc
     5281 gtcatcggcc aggtctcagc cgacgccgtt cgcgatgtcg ccgactcgac ccagtagcgg
     5341 gcttgttcgt agccttcaca ccaccgatga cgcttggcgg gctgtccaaa tcggcggccc
     5401 gctttcccct taccctttgt catcctgagg cgtgagccga aggatctctc ctacaccggc
     5461 ccgatggcta aacgaggaga g
//
LOCUS       DASUGJ010000644         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_940258, whole genome shotgun sequence.
ACCESSION   DASUGJ010000644 DASUGJ010000000
VERSION     DASUGJ010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940258"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..819)
                     /gene="pstB"
                     /locus_tag="VFV93_14720"
     CDS             complement(1..819)
                     /gene="pstB"
                     /locus_tag="VFV93_14720"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX5166654.1"
                     /translation="MSVVPQLTTHKLDADLAGANTIIEAEHVNIYYGNFLAFRDANLK
                     VPERKITSLIGPSGSGKSTLLRCFNRMNDLVPSARVEGRILFHGENLYDSDIDPVAVR
                     RLIGMVFQKPNPFPKSIYDNVAWGARINGFKGNMDELVERSLRSAALWDEVKDKLKSS
                     GLSLSGGQQQRLCIARCIAIQPEVILMDEPCSALDPIATLRIEDLMRELCEHYTILIV
                     THNMQQAARVSDMTAFMTMGEGRAGELVEVGPTRELFTSPKNPRTEDYITGRVG"
     gene            complement(829..1713)
                     /gene="pstA"
                     /locus_tag="VFV93_14725"
     CDS             complement(829..1713)
                     /gene="pstA"
                     /locus_tag="VFV93_14725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX5166655.1"
                     /translation="MSASTTRFNDAQLRRRKLIGNIALGLFILATLSALAVLAVLVGD
                     TLRDGLGWLDWDFIRNLPSRRPERAGIWPALAGTVWIMILTAAFTFPIGVGAAIYLEE
                     FGGKGRLARIIEINIANLAGVPSIVYGLLGLAVFVELSQLGPSVLAGALTMTLLVLPV
                     VIIASREAIRAVPDSLRQASLGLGATRLQTVWSHVLPAAMPGILTGTILALSRAIGET
                     APLIVVGAATYITYTPEGPTDRFTVLPMQIFEWISRPQAAFHALAAAGIIVLLALLLT
                     MNLAAILLRNYFRKNQNW"
     gene            complement(1710..>2490)
                     /gene="pstC"
                     /locus_tag="VFV93_14730"
     CDS             complement(1710..>2490)
                     /gene="pstC"
                     /locus_tag="VFV93_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX5166656.1"
                     /translation="TAGFFAEVSPVDFFTGTRWTPLFKPQSFGVLPLVVGTMQIAVFA
                     MLVSIPIGLASAIYLSMYAPPRIRAVVKPALEVLAGIPTVVYGYFALTFITPTVIKGL
                     FDDAPVFNALSAGIAVGIMTVPMVSSLSEDALQAVPRALRDGALALGATKFEVSTRVM
                     VPAALSGIMASIILAFSRAIGETMIVVIAAGATPKFTFNPLESIQTMTGFIVQISLGD
                     TPRGTIEYKTLFAVGFTLFLITLLMNILSALVTRRFREVYE"
BASE COUNT          484 a          773 c          832 g          401 t
ORIGIN      
        1 tcatcccact cgtccagtaa tgtaatcctc agtccgtggg ttcttcgggc tggtgaatag
       61 ttcgcgggtt gggccaacct cgaccagttc gccggcgcgg ccctcgccca tcgtcatgaa
      121 ggcggtcatg tcggagacac gcgcagcctg ctgcatgtta tgggtcacaa tcagtatcgt
      181 gtaatgctca cacagttcgc gcatcaggtc ttcaattcgc aacgtcgcga tcgggtcgag
      241 ggcggagcac ggctcgtcca tcaggataac ctccggctgg atcgcgatac agcgcgcgat
      301 gcagaggcgc tgctgctggc cgccggagag ggacagccca ctcgacttga gcttgtcctt
      361 gacctcgtcc cagagggcgg ccgaacgcag gctgcgctcg accagctcgt ccatgttgcc
      421 cttgaagccg ttgatccgcg ctccccaggc gacgttgtcg tagatcgact tcgggaacgg
      481 gttcggcttc tgaaagacca tcccgatcaa gcggcggacg gcgaccggat cgatgtcgga
      541 atcgtacaga ttctcgccat ggaacaggat ccgtccctcg acccgcgcgc tcggcacgag
      601 atcgttcatc cggttgaaac agcgcagcag cgtgctctta ccggagccag atgggccgat
      661 cagcgaggta atcttgcgct ccggcacctt taagttcgcg tcgcggaagg cgaggaagtt
      721 gccgtagtag atattgacgt gctcggcctc gatgatcgtg ttcgcgccgg cgaggtcggc
      781 atccagcttg tgggtcgtca gttgcgggac aacgctcatc gtggttccct accagttctg
      841 gttcttgcgg aagtagttgc gcaacaggat cgccgccagg ttcatcgtca gaagcagcgc
      901 cagcaggacg atgatgccgg cggccgccag cgcgtggaaa gcagcctgcg gacgggagat
      961 ccactcgaag atctgcatcg gcaggacagt gaagcggtcg gtcggtccct ccggagtgta
     1021 ggtgatgtag gtggctgcgc cgacgacgat cagcggggcc gtctcgccga tcgcgcgtga
     1081 gagagcgagg atcgtcccgg tcaggatacc cggcatcgcg gccggcaaaa cgtgcgacca
     1141 gaccgtctgg aggcgggtcg ctcccagccc cagcgacgcc tgccgcaacg aatccgggac
     1201 ggcgcgaatc gcctcgcgcg aggcgatgat gacgaccggc aggaccagca gcgtcatcgt
     1261 caatgccccg gccaggacgc tcgggccgag ctgcgacagc tcgacgaaga cggccagccc
     1321 cagcagcccg tagacgatcg acggtacgcc ggccaggttg gcgatgttga tctcgatgat
     1381 gcgcgccagc cgccccttcc cgccgaactc ctcaaggtag atggccgcgc caacgccgat
     1441 cgggaaggtg aacgcggccg tcaggatcat gatccagacc gttccggcca gcgccggcca
     1501 gatcccggcc cgctccggcc gccgcgaggg caggttgcgg atgaagtccc aatccagcca
     1561 gccaagtccg tcgcgcagcg tgtcgccaac caggacggcc aggacagcca gcgcgctcag
     1621 ggtggcgaga atgaacaggc ccaacgcgat gttgccgatc agcttgcggc ggcgcagctg
     1681 ggcgtcgttg aagcgggtcg tcgatgcgct cattcgtaga cctcccggaa gcgccgggtg
     1741 accaacgcgc tcaggatgtt catcaggagt gtgatcagga acagcgtgaa gccgacggcg
     1801 aagagtgtct tgtactcgat cgtcccgcgc ggcgtgtcac cgaggctgat ctggacgatg
     1861 aagccggtca tcgtctggat gctctccagc gggttgaagg tgaacttcgg cgtcgcgccg
     1921 gccgcgatga cgacgatcat cgtctcgccg atcgcccgcg agaaggccag gatgatcgag
     1981 gccatgatgc cggagagtgc agccgggacc attacccgcg tggagacctc gaacttggtc
     2041 gcgccgagcg ccagcgcgcc gtcgcgcaac gcgcggggaa cggcctgcag ggcgtcctcc
     2101 gagagcgagg agaccatcgg gaccgtcatg atcccgaccg cgattccggc actgagagcg
     2161 ttgaagaccg gggcgtcatc gaagagcccc ttgatgacgg tcggcgtgat gaaggtcagc
     2221 gcgaagtaac cgtagacgac ggttggaatg ccggccagca cctcaagggc aggcttaacg
     2281 acggcccgaa tgcgcggagg ggcgtacatc gaaagataga tcgcactcgc caggccgatc
     2341 gggatcgaga ccagcatcgc gaagacggcg atctgcatcg tcccgaccac cagcggcaaa
     2401 accccgaacg actgcggctt gaagagcggc gtccagcgcg tgccggtgaa gaaatcaacc
     2461 ggcgagacct cggcaaagaa gccggcggtc
//
LOCUS       DASUGJ010000645         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_800403, whole genome shotgun sequence.
ACCESSION   DASUGJ010000645 DASUGJ010000000
VERSION     DASUGJ010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800403"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..3497
                     /locus_tag="VFV93_14735"
     CDS             462..3497
                     /locus_tag="VFV93_14735"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5166657.1"
                     /translation="MKSANEIDARRRPTPLFETEVAPVSVQIQLLGGFRVVVDGRVVP
                     ETAWRLGNARRLIQVLALAPNHSLHREQIMDLFWPDLTPHAAASNLYQVVHAARRALN
                     ACYAPHGKPAAIIHLRQRVVILEPPGTLEIDVEAFDLAAGIARRTADPQRCREAVSLY
                     GGDLLPEHPYDEWLVGRRLVLRDTVTTLLFHEARLHGDAAAYDAAIAALRRIAEIEPE
                     NEQAQADLMRVLALNGQVALALNQFKRWSERLRSEVDEEPGEAIVLLNKDIAAGRIGP
                     DVISHPVSLPLTNLKEPLTSFVGRQAELAEVLALARQHRLLTLTGSGGCGKSRLARAA
                     ASTLTDAFPDGVWWVELAARTEPESVVNAVATAVKVIEERDRPLEETLVDALRHRRLL
                     IVLDNCEHLIERCAALCTTILESCPRVQILATSRQRLQVAGEQAWRVPSLTLPDTGAP
                     SPGQIHASEAVQLFVERARLAQSSFAIDAGNADIVATICRRLDGIPLAIELAAARVAV
                     LGPGQIAGRLDDTLQLLIAGNRSTSPRHQTLRAALDWGYGLLSEPQQGALTQLSVFAG
                     GWALEAAEAVLDGEQRGDGERQDLVLQLAEQSLVQVEQVGTDARYHFLEIVRQYARER
                     LEDSGQEKAIRDRHAAYYAQRAEATEHVLRGPDQADGLKWLDAEVENTRAALAWSCRS
                     GNAELGLRLCWGLWRYWQTRGSITEGRQWIARVLELPPPATCLELHAGVLFAAARFAL
                     LQSDEGATELARQCLTRSRAIGHQDHATGALTMLGHLAMQRGETATAREHYEEALRIR
                     RDLGEAWSIAISLNSLGLVALEEQEIDRARSLFDESLELFRAAGDTYHVANGLYYVGL
                     GGFLAGDHAAARRAYDESMAILRQLGGRPRIARGLVGLAQLAGAEGDRLNARALLAEA
                     LTIAVDIGSRETLVTCLEEFAILAAAGDPAAAIRLAAAAQALHNAAGEPPQRHMLDRL
                     NAGLEPARRLLTPSELTTAETTGARMSVDEASQFALGLR"
BASE COUNT          618 a         1268 c         1242 g          552 t
ORIGIN      
        1 caagagcagg tactccagcc gcgccagcag cggctcccgc tcaccggcca tcaggtcgag
       61 gaacagcggc tggacgtcga tcagcaccag cgccgcccgc tccggctcga cgaatcgact
      121 ggattccacc atcacacccc ctgacatcaa gcgcactgta acgtgcaagg tgtgcaagag
      181 gcaacgaggc agcaccacca gggcgcatcc caacacgttg cgctatctcc gtaggggccg
      241 cggctccgtc tcggcccgcg ccgtgaggcg ccttcgtcgg tcgtgcatcg atctacgcgg
      301 taggaattat tcctacctca gtacctacgc agcgcacacc atcagcacgc ctggcacgta
      361 caatgtgcgg aacgccaacg acacgactcc gctggcattc ccagggcgat ttcggtcgtc
      421 aacacggtcg ccggcgcatg acgagtggtg acagaccagg catgaagtcc gcgaacgaga
      481 tcgacgcgcg ccgcaggccg acaccgctgt tcgagacgga ggttgctccg gtctcggtcc
      541 agatccagtt gctcggcggc tttcgcgtcg tcgtcgatgg ccgggttgtg ccggagacgg
      601 cctggcggct gggcaacgcg cgccggctga tccaggtcct ggcgctggcg ccgaaccaca
      661 gcctgcatcg cgagcagatc atggatctgt tctggcccga tctcacgccg cacgcggcgg
      721 ccagcaatct ctaccaggtc gtgcatgccg ccaggcgtgc gctcaacgcc tgttacgcgc
      781 cgcacggcaa gccggccgcg atcatccacc tgcgccagcg ggtcgtcatc ctcgaaccgc
      841 ccggcacgtt ggagatcgac gtcgaggcct tcgacctggc ggccgggatc gcccgccgga
      901 cggctgatcc gcaacgctgt cgcgaggccg tcagcctcta cgggggcgac ctgctgccgg
      961 agcatccgta cgacgaatgg ctcgtggggc gacgactggt gctgcgcgac accgtcacga
     1021 cgctcctgtt tcacgaggct cggctgcacg gggacgccgc tgcctacgac gcggcaatcg
     1081 cggcgctgcg gcgcatcgcc gagatcgagc cggaaaacga gcaggcccag gccgacctga
     1141 tgcgcgtgct ggcactgaac ggccaggtcg cactggcact gaaccagttc aagcgctgga
     1201 gcgagcgcct gcgcagcgag gtcgacgagg aacccgggga ggcgatcgtc ctcctgaaca
     1261 aagatatcgc ggccggccgt atcggacccg acgtcattag ccatcccgta tcactgccgc
     1321 tcaccaatct caaggaaccg ctgaccagct tcgtcggccg gcaggctgaa ctggcggagg
     1381 tactggcgct ggcgcggcag catcgactcc tgacgctgac cggctcgggc ggttgcggga
     1441 agtcgcggct ggcgcgagcc gccgcgtcca cgctgaccga tgcgtttccg gatggtgtct
     1501 ggtgggtcga gctggcggcg cggaccgagc cggagtcggt cgtcaatgct gtcgcgacgg
     1561 cggttaaggt catcgaggag cgcgaccgcc cgctggagga gacgctcgtc gacgcgctgc
     1621 gccatcgacg gctgctgatc gtgctggaca actgcgagca cctgatcgag cgttgcgccg
     1681 cactctgcac gacgattctt gaatcgtgcc cgcgagtcca gatcctcgcg accagccgcc
     1741 agcggctcca ggtagccggt gaacaggcct ggcgggtgcc gtcactgacg ctccctgata
     1801 cgggcgcgcc gtcgccggga cagatccacg ccagcgaggc ggtccaactc ttcgtcgagc
     1861 gcgcgcggct ggcccagtcc tccttcgcga tcgacgccgg gaacgcggac atcgtcgcca
     1921 cgatctgccg gcgactggac ggcatcccgc tggcgatcga gctggctgcc gcgcgggtcg
     1981 ccgtgctcgg tcccggccag atcgccgggc ggctggacga cacattgcag ctgctgatcg
     2041 cgggcaaccg ctcgacctcg ccgcgccacc agacgctgcg ggccgcactc gactggggct
     2101 atggcctgtt gagcgagcca caacaaggcg cgctgaccca gctgagcgtc ttcgccggag
     2161 gctgggcgct ggaagccgcc gaggcagtgc tggatggcga gcaacgcgga gatggggaac
     2221 gccaggatct cgtcctgcag ctggcggagc aatcgctcgt ccaggtcgag caggtcggaa
     2281 ccgacgcacg gtatcacttc ctcgagatcg tgcgccagta cgcccgcgag cggctggaag
     2341 acagcggcca ggagaaggca atccgcgacc ggcacgccgc ctactacgcc cagcgggcgg
     2401 aagcgactga gcacgtccta cgcgggcccg atcaggcaga cggcctcaaa tggctggacg
     2461 cggaggtcga aaacacccgg gcggcgctgg cctggtcgtg tcgatccggc aatgcggagc
     2521 tgggactgcg gctctgctgg ggcctctggc gctactggca gacgcgtgga agcatcacgg
     2581 agggtcgcca gtggatcgcg cgagtgctgg agctaccgcc accagcgacc tgcctggagc
     2641 tgcacgccgg ggtgctcttc gcggcggccc gcttcgcgct gctccagagt gacgagggag
     2701 cgacggagct ggcgcggcaa tgcctcacgc ggtcgcgcgc gatcggccac caggaccatg
     2761 cgaccggcgc gttgacgatg ctcggccacc tggcgatgca acggggcgag accgcgaccg
     2821 cgcgcgaaca ctacgaagag gcgctgcgca ttcggcgcga cctcggcgaa gcctggtcga
     2881 tcgcgatctc gttgaactcg ctcggtctgg tcgcacttga ggaacaggag atcgatcggg
     2941 cgcggagcct gttcgacgag agccttgagc tgttccgcgc ggccggcgac acctaccacg
     3001 tcgccaacgg gctctactac gtcggcctcg gcggcttcct ggccggcgat cacgctgctg
     3061 cacgtcgcgc ctacgacgag agcatggcga tcctgcggca gttgggcggg cggccccgca
     3121 tcgcgcgcgg tctggtcggg ctggcccagc tcgcgggggc cgagggggat cggctcaacg
     3181 cacgcgcgct gttggccgag gcacttacca ttgccgtcga catcggctcc cgcgagacgc
     3241 ttgtgacctg cctggaggag ttcgccatcc tggctgcggc cggcgacccg gccgcagcaa
     3301 ttcgactggc cgctgcagct caggcgctcc acaacgccgc cggagaaccg ccccaacgac
     3361 acatgctgga tcgcctgaac gctggcctgg aacccgcgcg acggctgctg acaccgtccg
     3421 aactgaccac tgccgaaaca accggcgcgc gcatgagcgt cgacgaagcc tcccaattcg
     3481 cgctcggatt gagatagggc aattgtcgaa cggccttcct catgtcgcca acgatgacaa
     3541 gccctcgctg cccctcgcgc tcacgctcta ccgcaggcac aacctcggct tcgccgacgc
     3601 attactggcg gcccgttcgc tcatccgagg tgatacggag atcatctcgt ttgatcgtgg
     3661 actcgaccgc gtgcccggtc
//
LOCUS       DASUGJ010000646         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2051544, whole genome shotgun sequence.
ACCESSION   DASUGJ010000646 DASUGJ010000000
VERSION     DASUGJ010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051544"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..>2836)
                     /locus_tag="VFV93_14740"
     CDS             complement(149..>2836)
                     /locus_tag="VFV93_14740"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166658.1"
                     /translation="EIESLEQYALMLRLPGPLSIGSHDVAVTYGSATDANPDSISVID
                     SDRCDGGTEFASAGALGVLSQGGVATATATISQDAGSAAFALTWQGSDFDLSLTAPSG
                     RPISETSTDPDVFVTQGLSSVEIRVADPETGEWDLRAEGIDVPSPEPVSYAVSEIDPA
                     IQTELTVTGDQAGLPLEVRLAVDGSSGPLAGADVLARITDPAGTTRRFTLSDDGAHLD
                     GTRSDGVYGVEAWATALAGAYSIEVAVSGTDGNGEPYARLEAATVSLGQITDTDSDGV
                     ADGAELRYGLNPGNPGDGDTDHDFDGIGLAVELAQGTDPFGWDSDHGGESDRSEVAAG
                     TDPLDPSDDAALPTPWLSLALLDNHRVAVSAGASDGSADVRLYRITGGALADLGLRPG
                     AGSTLEDGPLAAGSYGYTAVALSGVHESAPVVAGPVTPVADATSPFVRIVLNDGVWET
                     GDPEVRVRFTDLSGPVAEMRLAESIDELAIAPWVPFVNPTTFTVEGTPGTHPVVAQVR
                     DANGVESNVAYGAVDLLGGASWSAPVVVNDVLTNFQFTPSVALGPGNTAYAAWEDRRT
                     NGDHDIYFSKRDPTTGTWSANERVNNVTTQTQDDPAIAVDAAGNAYVVWTDTRNGATD
                     HDIYFSKRSASTGAWSPSVRVNDDGAGKRQDFPSIAVTSSGDAVAVWRDQRGGGNKKN
                     IYSARLPAGSSTWSANLKVTTESNAHKEEPQVAIGSNGTAYAVWADQRNSGNQDIWFA
                     SLASGASTWSANTQISETPNGGKEDPAIGIDSAGNLVAVWDSGSIKARGRPAGTSTWD
                     PVVTVGGSDVNLPSIAVRGDGRAFVAWYQGLATTLTTLHASEYQPGTGTWTSPELLTD
                     PAVESGTPSVAVDSSQVIVLYHGRPAGGSYEIYAKRKGL"
BASE COUNT          470 a          896 c          964 g          506 t
ORIGIN      
        1 gaacggtacc gccacgagcc ggacagggct ttcatacagg agataactac ccgaaaccag
       61 cctgattgct tgcctgcccg ttgtgctcag catctgaaag ccgcgcgtgt ccgacgggtg
      121 agtctgcaag gggtcgggga cagtatgcct acaggccctt tcgcttggcg tagatctcgt
      181 agctaccgcc ggccggtcgc ccgtggtaga ggacgatcac ctgggacgag tcgacagcga
      241 ccgatggggt tccggactcg acagccggat ccgtcagcag ttccgggctg gtccaggttc
      301 ctgtgcccgg ttggtattcc gaagcgtgga gcgtcgtcag cgtggttgcg aggccttggt
      361 accacgcgac gaaggctcgt ccgtcaccgc gaacagcaat cgatggaaga ttgacgtccg
      421 agccgccgac ggtgacgacg ggatcccacg tcgacgtgcc agcaggacgg ccgcgcgcct
      481 tgatgctgcc actgtcccag acagcgacga ggttcccggc gctgtcgatg ccgatggccg
      541 gatcttcctt accgccgttt ggagtctcgc tgatctgagt gttggccgac caggtggagg
      601 cgcctgacgc taacgaggcg aaccagatgt cctggttgcc ggagttccgc tggtccgccc
      661 atacggcgta ggctgtgccg ttggagccga tcgcgacctg cggttcctcc ttgtgggcgt
      721 tcgactcggt cgtgaccttc aggttggccg accaggtcga cgaccccgcc gggagccttg
      781 ccgagtagat gttcttcttg ttgccgccgc cccgctgatc gcgccagacg gcgaccgcgt
      841 cgcccgagga ggtcacagcg atcgacggga agtcctggcg cttgccggcg ccgtcgtcgt
      901 tgacacgaac gctcggactc caggccccgg tggatgcgga ccgcttcgag aagtagatgt
      961 cgtgatcggt ggcgccgttc cgggtgtcgg tccacacgac ataggcgttg ccggccgcgt
     1021 cgaccgcgat cgccgggtcg tcctgcgtct gcgtcgtcac gttgttaacg cgctcgttcg
     1081 cgctccaggt gccggtcgtc ggatcgcgct tcgagaagta gatgtcatgg tcaccgttgg
     1141 tccgccggtc ctcccaagcg gcgtaggctg tattacccgg tccaagagcg actgatggag
     1201 tgaactggaa attggtcagg acgtcgttga caacgaccgg tgcggaccag ctcgctccgc
     1261 caagcaggtc caccgcgccg taggcgacgt ttgattcgac gccgttcgcg tcgcgaacct
     1321 gggcgacgac tgggtgcgtc cccggcgtgc cttcgaccgt aaaagtcgtc gggtttacga
     1381 acggcaccca tggtgcgatg gcgagctcgt cgatcgattc cgcgagccgc atctcagcga
     1441 ccgggccgga gaggtcggtg aatcgaactc tcacttcggg atctcctgtc tcccagacac
     1501 cgtcgttcag gacgatccga acgaagggcg aagtggcatc ggcgacgggt gtcaccgggc
     1561 ctgcgacgac gggcgcggac tcgtgaactc cggacagagc gacggcggtg tagccgtacg
     1621 agccagcggc gagtggtcca tcctcgaggg tggatccggc tccaggtcgc agtcccaggt
     1681 cggccaaggc accgccggtg attcgataca ggcgcacgtc ggcggacccg tcggacgcgc
     1741 cagctgagac cgcgacccgg tggttgtcga ggagcgcgag gctgagccat ggcgttggaa
     1801 gggccgcatc gtcggatgga tcaagagggt cagtcccggc cgcaacctcg gagcggtcgc
     1861 tctcaccgcc gtgatcggaa tcccagccga agggatcggt cccctgggcc agctcgacgg
     1921 ccaagccgat gccgtcgaag tcgtgatcag tgtcaccgtc gccaggattg ccggggttga
     1981 gcccgtagcg cagctcggcg ccatcagcca cgccatcgct gtcagtgtcg gtaatctgcc
     2041 ccagagagac agtggcggct tcaaggcggg catagggttc tccgttgccg tcggttccag
     2101 acaccgcgac ctcgatcgaa tacgcgccag cgagcgcggt cgcccaggct tcgacaccgt
     2161 agacgccgtc ggacctcgtt ccgtcaaggt gcgcgccgtc gtcgcttaac gtgaaccggc
     2221 gggtcgtgcc agcgggatcg gtgatgcgcg cgagcacgtc cgcaccggcg agcggaccgc
     2281 tcgacccgtc cacggcaagt cggacttcga gtggcaggcc ggcttgatcg ccggttacgg
     2341 tgagctcagt ctggatcgct gggtcgatct ccgagacggc gtatgagacg ggctccggcg
     2401 acggaacgtc gatgccctcg gcgcgcagat cccactcgcc cgtctctggg tcagcgacgc
     2461 ggatctcaac gcttgatagg ccctgcgtca cgaagacatc gggatcggtg gacgtttcgg
     2521 agatcggacg tccactcgga gcggtaaggc tcaagtcaaa gtcggagccc tgccacgtga
     2581 gtgcgaatgc cgccgaaccg gcgtcctggc tgatcgtggc tgtggctgtc gccacgccgc
     2641 cttggctcag gacacccagc gcgcccgcgg aggcaaactc ggttccacca tcacagcggt
     2701 ccgagtcaat caccgagatg ctgtccgggt tggcgtcggt cgccgatcca tacgtcacgg
     2761 cgacatcatg ggagccaatg gatagtgggc cgggcagccg cagcatgagc gcgtattgct
     2821 ccaggctctc gatttc
//
LOCUS       DASUGJ010000647         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_245980, whole genome shotgun sequence.
ACCESSION   DASUGJ010000647 DASUGJ010000000
VERSION     DASUGJ010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245980"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="VFV93_14745"
     CDS             complement(<1..1787)
                     /locus_tag="VFV93_14745"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5166659.1"
                     /translation="MPDKTDGPESALAGEAVQLFLERARLVRPEFVLTPESAGGVVEL
                     CRRLDGLPLAIELAAARVRHLAPASILARMDRQLSLLTGGPRDVPARQQTMEATIAWS
                     YDLLSSGQQRLFNQMAVFPGGWTLDAAEAVADVDFDLLDGLSELVDHSLVLATDDGGG
                     VTRYRMLEPIREFAWELLHRSGEADVLRYRHASYFLTLARKVEHQLGGPDEDRWASLI
                     QAEIDNLRTAFRWGIEHDVGLALEAVAALEWFWLPSGLMTEGRRWLTEAHQASQGVPG
                     HIVAKSLNVLGTLMGWQGDLDNARTSIERALALYEQQGDRQGIAKAQLNLARTYRFLG
                     DSTSARQLNEQSLANYRVLGERRWMAAALGNLANSFADLGDLDRAIEMMEEALSIELE
                     IGATSGARVSLSNLGTLELDRGNADQAIQRFTESLTLNHRTGKPRELRDSRFDIEDLE
                     GIAAVILESDPLRAAQLLGAASALRERTDYPMNYHAIRERHKRLTERLSALLDTATLA
                     SAWAHGMMMTTDDAIHVALTERHQAPQSITPAEHMKLQALSRRELDVLRGIVDGKTNQ
                     EIAADLFISPNTVTNHVTSILNKLGLDSRT"
BASE COUNT          431 a          758 c          802 g          485 t
ORIGIN      
        1 gtccgggagt cgagcccgag cttgttgagg atgcttgtta catggttcgt gaccgtgttg
       61 gggctgatga acaggtccgc ggcgatctcc tggttcgtct taccgtcgac gatgccgcgc
      121 aggacgtcga gttctcgtcg ggaaagcgct tggagcttca tgtgctcagc cggcgtgatc
      181 gattgcgggg cttggtgtcg ctccgtgagc gcgacgtgga tcgcgtcgtc cgtcgtcatc
      241 atcatgccgt gtgcccaggc ggatgcgaga gttgccgtgt cgagcagcgc gctgagtcgc
      301 tccgtcaggc gtttgtggcg ctcacgtatg gcgtggtaat tcatggggta gtcagttcgc
      361 tcccggagtg ccgacgcagc gcctagcaac tgtgcagccc ggagcgggtc actctcaagg
      421 ataaccgctg cgatcccttc taagtcttcg atatcaaacc gactgtcacg taactcgcgt
      481 ggtttgccag ttcgatggtt gagtgtgagg ctctcggtga accgctggat ggcctgatcg
      541 gcattccctc gatcaagttc cagagtgccg aggttagaga ggcttactcg cgcaccggac
      601 gtggctccaa tctcaagttc aatagacagg gcttcttcca tcatctcgat cgcgcgatcg
      661 aggtcgccga gatcagcgaa tgagttggcg agattgccga gtgctgcagc catccagcgt
      721 cgttcgccaa gcacacgata gttagccaga ctctgctcgt tgagttgccg cgccgaagtt
      781 gaatctccga ggaaccggta ggttcgtgcc aggttcagtt gcgccttggc gattccctgc
      841 cgatctccct gttgctcata gagtgccagt gcacgctcga ttgacgtacg tgcattatcc
      901 aggtctcctt gccaccccat caaggttccg agcacattga gactcttggc gacgatgtgg
      961 cccggcaccc cctgtgatgc ctgatgcgct tcggtgagcc accggcgccc ctccgtcatc
     1021 aatccgctcg gtagccagaa ccattcgagc gccgcaacgg cctcaagtgc caagccgaca
     1081 tcatgctcga taccccatcg aaacgcggta cgcagattgt cgatctcggc ctgaatcagg
     1141 ctggcccatc tgtcttcgtc tggcccgccg agttggtgct caaccttccg ggcaagcgtg
     1201 aggaaatagg acgcgtggcg gtaacggagt acatcagctt cgccgctccg gtgaaggagt
     1261 tcccacgcga actcacgaat cggttccagc attcggtaac gggtgacgcc acctccatcg
     1321 tcagtggcca gcacgaggct gtgatcgacc agttccgaga ggccatcgag gagatcaaaa
     1381 tcgacatctg cgacagcctc ggcagcgtcg agggtccagc cgccggggaa gaccgccatc
     1441 tggttgaaga gccgctgctg cccggagctg aggaggtcgt agctccaagc gatggttgct
     1501 tccatcgtct gctgtcgcgc cggtacgtcg cgcggacctc cggtcagcaa tgatagctgc
     1561 cggtccatcc gcgcgagaat cgaggctggt gccaggtgcc gaacccgggc cgccgcgagt
     1621 tcgatcgcga gcggcaggcc atcgagccgc cggcacagct cgacgacgcc tccagcgctt
     1681 tcgggggtca gcacgaactc ggggcgaacc agacgcgcgc gttccagaaa gagctggacc
     1741 gcctcgcccg cgagcgccga ttcgggcccg tcggtcttgt ccggcaacct gagtggcggg
     1801 acgaccagca ggtgctcgct gctgagccgg agcgggatgc ggcttgtaac gagcaatcgc
     1861 agtgacggac acgcagccag cagcgtcgcc agggccggcg cggcctctac cacctgctcg
     1921 acgttgtcca gcaccagcag gagcgcgcgg tccgtgattg ccgcgatcag ggcatcgagg
     1981 gccgttcccc cggccggctc ctgcaccccg agggcagccg cgacggcagc cgggacacgc
     2041 tcgtgggccg tgatggccgt gagtgggacg atcacgatgt cggtgttgtg gtcgcgcccg
     2101 tatctgtctg ccgcagccag tgccaggcgt gtcttgcctg agccgccggc accggtcagc
     2161 gtgaccaggc ggtgatgggc gaggaggtcg acggtgcgcg cgacttcctc cgtgcgtccg
     2221 atgaagctgg tggttgatgt caggaacgac atggcagaga ccgccgctca tgctccgatg
     2281 caacgcgatg gatgcatgat agcgcggatg gtttgcgctg tggccaccgt tgagccatgg
     2341 ccgattcgaa atcgcagggc gtgtgctacg ctgctcctga acatccatct gtgatgcaac
     2401 ccagggagcg caatatggtg actcgtcatc tccgcgcgga catggggatc ctctggctcg
     2461 atgacccacg ctgcac
//
LOCUS       DASUGJ010000648         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_940406, whole genome shotgun sequence.
ACCESSION   DASUGJ010000648 DASUGJ010000000
VERSION     DASUGJ010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940406"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VFV93_14750"
     CDS             <1..1488
                     /locus_tag="VFV93_14750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5166660.1"
                     /translation="HHSVQTTGALVNVLVISTGQPAIEWLALAISIRGHTPLRAADGP
                     AALTRLTQQPCQLVIIDEIDGGDDERLELCRQLRALPEMRTTPIWVTAGADDLAGIVT
                     AIDAGATSYLTRPYDARQLRARFELLERSLAPDSEPSTREPDDLEMLLEVLPDVVAIV
                     DPIGDLLLITSNVRLLLGFSNSELTGLNIEALCHPDDVPAVLGQIRAAAARPGLPAPS
                     AVRLQRANGSWQSVDLYCRDLRALPAVGGLLLILRAGSSRERQQQPTERATLIDPQTG
                     LASSALFTEHLSHALARGERLNHPTAVILLEMAVADEAETTRIARRIQRAIRAADIAA
                     RLDDGTFAILVEDLEDDDSEQMLARRIQDAFAGSGTATIGVARSRPGLTSAGELLRDA
                     GRALRQSNPRPAEPSASTASKPPPADPDPEQPDTQEPPRSTATCSEHSPPDMPRTRAP
                     NAGGDPPSIDLLRASLRPLEEHLTVIDRFRALEDHLAQLLNEDTR"
     gene            1773..2591
                     /locus_tag="VFV93_14755"
     CDS             1773..2591
                     /locus_tag="VFV93_14755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5166661.1"
                     /translation="MQQSEPVSDTSRTSLSGAALFAIHDLKGAVHAIQGFLSVLRSQH
                     AGPLTETQQDFLSSAFITARRIERLVGDLQVYEFGQQSLSLIPEDCDLLTILRMCIRE
                     LEPTATGYDVSVELCADANQNWRLFADPVRLEQIAFNLIENAIRYSTPHSTVVVRTRQ
                     SRSRLLLVVENEVADPTGINLERLFEPFQRGQAASESGRRGLGIGMTVVKTLVAARGG
                     DVYARVRGTTVSIAASLPRRSPAVAASSSGSDSAVTPGTRARTLPTAQARSGSY"
     gene            complement(2820..>3318)
                     /locus_tag="VFV93_14760"
     CDS             complement(2820..>3318)
                     /locus_tag="VFV93_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166662.1"
                     /translation="ALDDPPLGYTIYWLGARVNNGGPLPALELTHVSRYNPGDGSWPA
                     DQVVLEYGDPSQPFVDQPVVRIFEYSREVWARIPATTHGPDGPCWSTEEVRLADGRAT
                     LFAGFSWPDDVPPDPHDPDACPTDREHDRFFAHAFLGDTVVVIDIPFDSRASLDAALM
                     ALKPR"
BASE COUNT          598 a         1148 c         1010 g          562 t
ORIGIN      
        1 catcattcgg ttcagacgac aggtgcgctc gtgaacgtcc tcgtgatctc aactggtcag
       61 ccggcaatcg agtggctggc gcttgcgatc tcgattcgcg gccacacccc gttgcgggct
      121 gccgacgggc cggcggcgct gactcgcctg acccagcaac cctgccagct cgtcatcatc
      181 gatgagatcg acggcggcga cgacgagcgg ctggaactct gccggcagct ccgcgccctg
      241 ccggagatgc ggacgacgcc catctgggtc acagccggcg cagacgatct ggccggcatc
      301 gtcacggcca tcgacgctgg tgcgaccagc tacctcaccc gcccgtatga cgcccgccag
      361 ctccgcgccc ggttcgagct cctggagcgc tccctcgcgc cggacagcga gccgtcaacc
      421 cgcgagcccg acgacctcga aatgttgctg gaggtgcttc cggatgtcgt cgcgatcgtc
      481 gacccgatcg gcgatctgct tctcatcacc tccaatgtcc ggctgctgct cggcttcagt
      541 aacagcgaac tgaccggcct gaacatcgag gcgctctgcc atcccgacga cgtgccagct
      601 gtgctgggcc agatcagggc ggcggcagcc cgaccaggct tgccggcgcc gagcgccgtc
      661 cgcctgcaac gggcgaacgg gagctggcag tcggtcgacc tctactgccg cgatctgcgg
      721 gcgcttccag cggtgggcgg tctactgctg attctgcgtg ccggatcgtc ccgggagcgc
      781 cagcagcaac caaccgagcg agcgaccttg atcgacccac agaccgggct cgccagttcg
      841 gcgctgttca ccgagcatct gtcccacgcg ctcgcgcgcg gggaacggct caaccaccca
      901 accgccgtca tcctcctgga gatggccgtt gctgacgagg ctgaaacgac ccggatcgcg
      961 cgccggatcc agcgcgcaat tcgggcggcc gacatcgcgg cccggctgga tgacggcacc
     1021 ttcgccatcc tggtcgagga cctcgaggac gacgacagcg agcagatgct cgcgcggcgc
     1081 attcaggacg cgttcgcagg gtccggcaca gcaaccatcg gggtcgcccg cagccggccc
     1141 ggtcttacca gtgccggcga actgctgcgc gatgccggcc gtgcattgcg ccagtccaac
     1201 ccccggcccg ctgaaccatc cgcctcaacc gccagcaaac ccccgccagc agaccccgat
     1261 ccggagcaac ccgacacgca ggaaccgcct cgatcaaccg cgacatgctc tgagcattcg
     1321 ccgccggaca tgccgcgcac gcgggccccg aacgctgggg gcgacccgcc gagcatcgat
     1381 ctgctgcgag cgagcctgag accgctggag gaacacctga cggtcatcga ccgcttccgc
     1441 gcgctcgaag accacctcgc ccagctcctc aacgaggaca cccggtagcg cgaacgcatg
     1501 cgcggactcg cgattgtgga cctcagatcg cgattcaatg cgcccgcacc ctgtatcacc
     1561 atatccatct attgtaaact tgacgtatcg aacacacgca gccgattctc aatctggtct
     1621 ggcgcgaact cctgggacaa ttggagacac cggccgcatg ctggcggcac aatcgaggtc
     1681 cacgttatcg cagttgggtg actcaatgcg atcaatgccg ggtccagact gtgttggtca
     1741 cacagccatc ccggtggtgt tggagctaag caatgcagca atcggagcca gtttcggaca
     1801 cgtcacgcac gtccttgtcg ggcgccgccc tgttcgccat ccacgacctg aaaggcgcag
     1861 tacacgcaat ccagggattt ctcagcgttc tgcgcagcca gcacgccggc ccgctgacgg
     1921 aaacccagca ggatttcctc tcctcggcct tcatcacggc ccgccgtatc gagcggctgg
     1981 tgggagatct gcaggtctac gagttcggcc agcagtcact ctcgctcatt cctgaggatt
     2041 gcgacctgct gacgattctg aggatgtgta tccgcgaact ggagccaacc gcaactggct
     2101 atgacgtttc ggtcgagcta tgcgccgatg cgaaccagaa ctggcgcctg ttcgccgatc
     2161 cggtacggct cgagcagatc gctttcaact tgatcgagaa cgcgatccgc tattcgaccc
     2221 cgcattccac cgtcgtcgtc aggacacgcc agtcgcgctc acgcctgctg ctggttgtcg
     2281 aaaatgaggt cgccgatcca accggcatca atctcgaacg gctgttcgag ccgttccagc
     2341 gcggccaggc ggccagtgag tctggccgtc gcgggctggg tatcgggatg acggttgtca
     2401 agacgctggt cgctgcgcgg ggcggcgacg tgtacgcccg ggtacgcggc acgaccgtct
     2461 cgattgcggc tagtctgcca agacgaagtc ctgcagtggc cgcatcatca tcagggtcag
     2521 attcagcggt aacgcctggt acgcgcgcac ggactctccc cacagcccaa gcacgctccg
     2581 ggagttactg acgtggatca tcattcctgg tggtagattc agcgcgcccg caatgcggct
     2641 gatggtatcc ggcttcccct cgacgaccag accgttgcgc gtttcagtcc agtactctcg
     2701 caccaaccgt ttcccccttc ggcgtgcaac ccgtacccat cgtctccccc atgtgatgga
     2761 cactgtacgt tcagtctaca ccgttccggc cccgttgcca actggtcaca atcacattgc
     2821 tagcgtggct tcaatgccat cagcgcggcg tcgagactgg cccgcgagtc aaacgggatg
     2881 tcgatcacga cgacggtgtc gcccagaaac gcatgggcga agaaccggtc gtgctcgcga
     2941 tccgttgggc aggcatcggg atcgtgcggg tccggtggca catcgtccgg ccaggagaag
     3001 ccggcgaaga gcgtggcacg gccgtcagcc agccgcacct cctcggttga ccagcacggc
     3061 ccatcagggc catgcgtcgt cgccggaatc cgagcccaga cctcgcgtga gtactcgaag
     3121 atccgcacta ccggctgatc gacaaacggc tgggacggat cgccgtattc gaggacaacc
     3181 tgatcggccg gccaggagcc gtcgcctggg ttgtagcgac tgacgtgcgt taactcgagg
     3241 gcaggcagag gccccccgtt attgacgcgg gcgccgagcc agtagatcgt gtaccccagc
     3301 ggcgggtcat ccagcgcc
//
LOCUS       DASUGJ010000649        15039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1078965, whole genome shotgun sequence.
ACCESSION   DASUGJ010000649 DASUGJ010000000
VERSION     DASUGJ010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15039
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078965"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFV93_14765"
     CDS             complement(<1..380)
                     /locus_tag="VFV93_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166663.1"
                     /translation="MTVTTAVNAFISDVKAILAEDGSAEPGLQRIATRMESFVRDPDV
                     LASHEDFTGNIHTGRQSAPLYTDEGGLTLVRARFGPEAMTPIHSHGSWGVIGVYKGRD
                     RYQVWQRNDPGSGDGHAEVEMVNER"
     gene            complement(395..784)
                     /locus_tag="VFV93_14770"
     CDS             complement(395..784)
                     /locus_tag="VFV93_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166664.1"
                     /translation="MPVDTQTLPGTIYRGENGEELARGWCLVTYEMVGNALPLDDWRG
                     EIAVTTDEYDASAGWGDELYIRFRPYGGVWEPWHGPVTVEQVDPEIDPNRRRLRLRPA
                     GPLTRSRYTPDELAHGWPAKDETPAAR"
     gene            complement(926..1510)
                     /gene="lepB"
                     /locus_tag="VFV93_14775"
     CDS             complement(926..1510)
                     /gene="lepB"
                     /locus_tag="VFV93_14775"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5166665.1"
                     /translation="MAAAGEHNAAMNAEPTQLDEAERPDGSFRRNWLPTLRFLLLCVA
                     IWLGLRVVAPSYAIEGESMWPSLHDGGRVILNGAYRFQSAHHGDVVVFNPPIESDKPY
                     IKRVIGLAGDTIDIHDGAVYRNGIRLDEPYINGAETDCFHAPYCSLTVPAGMIYVLGD
                     NRPNSSDSRVFGPVDRDAVLGDVLFSFWPLDDIH"
     gene            complement(1648..2607)
                     /locus_tag="VFV93_14780"
     CDS             complement(1648..2607)
                     /locus_tag="VFV93_14780"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX5166666.1"
                     /translation="MGTLERPARRSFSFNPIFRKLILLATHNRLVERIVRRYGLRLGG
                     SRFVAGESLDAAVPVLRSLNQRGLVTNTTLLGEGVRDAAAAAAVVTAYKALLDRLAAE
                     GLQTNIALKLSHLGLDLGEETAYANLEQIVSHAATLGNFIRIDMEESARVDATLRIYR
                     RLRDAGHVNVGTVLQSALYRSERDLEALLPLAPNLRLVKGAYLEPASVAYPQKADVDR
                     NYIRLAERMLAGDGYTAIATHDERIIEHVIDFTGRNGIARDRFEFQMLYGIRSRYQTE
                     LASRGYKVLVATPYGSEWYFYLMRRLAERPANVLFLLRGIIKR"
     gene            2848..4278
                     /locus_tag="VFV93_14785"
     CDS             2848..4278
                     /locus_tag="VFV93_14785"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5166667.1"
                     /translation="MSRSQRRGAEVRIILLAVWLMVATLLTACSGPESGQDARTTPMA
                     TRTATSVSGVPTSTSAATETATATIPAVVETPGEPTATVQAGTPTEPPNPTATATPIT
                     PTQELTPTPQPPIDLGQVQISVEQVGSGFSQPVTLTHAGDGSGRRFVVEKTGMIRLLD
                     GSVYLDLTDRVLAYDVRTTQHELGLVGLAFHPDFETNHYFYVHYTNQDQDHVISRFSE
                     GADGLGDPASEKVLITYDQPDVNFFGGTLAFGPDGYLYIGMGTGTSVDADQIVAQQLD
                     NLYGKILRIDVNSGDPYGIPPDNPFVNTPGARPEVWAYGLRNPWRFSFDPANGDVYIG
                     SPGEFAREWINYIPAGSAPGKNFGWPILEGGECWEYWTGDCTTEGFELPIYVRPTYQD
                     GNCVILGGFVYRGQLSPSLNGVYLFSDYCSGRIWGLARDAAGVWQSAELYNLAALVSS
                     FGEDEAGEIYIFEIENGIVYRIVGTR"
     gene            complement(4381..5211)
                     /locus_tag="VFV93_14790"
     CDS             complement(4381..5211)
                     /locus_tag="VFV93_14790"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5166668.1"
                     /translation="MDNVLDQPACDELTDDRIGAGSTSNARRLHVLADYLSAEAELET
                     QQIILPASGRKLAIRKPTEAARERMFEAARLDPDKQMPYWARIWPSGVALADIALERR
                     AELAGQRVLELGCGLGTTASTLLEAGAILTVSDYSPLPLAFCRYNALSNTGRSPRTLR
                     FNWRTPDDGAVARAASGGGYPLILAADVLYEGRDVEPLLAIVERILAPDGMLWLAEPG
                     RKTAQRFLNTAAALGWHGDTQTVPGPWPDGSNDTISLHFLRRPTDIDQLTTNLGGWRT
                     "
     gene            complement(5241..6488)
                     /locus_tag="VFV93_14795"
     CDS             complement(5241..6488)
                     /locus_tag="VFV93_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166669.1"
                     /translation="MFDSTALLGLFRPFRYLAGDGRALSFWSARTLELLADWVLLVAL
                     FVAVYAVTADIAVVALFMLLRVLPRAVISLGFHKPATGISPDWWFLLGLSRVPLIASL
                     ALLDSRADLVWAGVVGVMYGLLTALSNESRAATLPRIVPRTRLALATHLNASIERITF
                     VAGPLLAATILRGWDIEVALLASAGLVLVASVLLWLQPPVAIDPAMQPASSLHNDESV
                     LIVARHQPGLLLLASGLFIGSALAICLKVVLIELAAEPLNRSDETLGLLLALVGAGTL
                     LGPLSVPRLLGHLPVSLIVTLSAIGIAAGIILMSIVTRIEIVVLVLLGIGFISITNDL
                     VTATAIRRIAPETDLPGAGRLMLVAVIAGQIVAAIAVALMARFWDSTDVMLAVGVVCA
                     LLMGILYLASDGMTLETRRTARS"
     gene            6706..7983
                     /locus_tag="VFV93_14800"
     CDS             6706..7983
                     /locus_tag="VFV93_14800"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HEX5166670.1"
                     /translation="MTTEAIFTMDTSSIKYGAGATAEIGFEMARLGAGRVLVVTDPNL
                     RDSDAVGKALASLSAAGIESVVYDRVRVEPTDVSFREAIEFASDGGFDGYVGIGGGSA
                     IDTAKVANLFATWPADLLTYVNAPIGQAKPVPGPLKPLIAIPTTAGTGSETTGVAIFD
                     LTEMHAKTGIAHRALRPVMGIVDPDNTRSMPPMVAACSGFDLLCHAIESLTALPYSER
                     PAPEHPSVRPAYQGANPISDIWASKALELTAANIVRAVQNPDDDAARAAMLLAATYAG
                     IGFGNAGVHLPHGMSYPVSGMVRDFRPEGYQVDHAIIPHGMSVILNAPAVFRWTASAN
                     PERHLYAAGLLGAETHGVNPDEAGELLATTIIDLMRATGMPNGLAAVGFEGDDIDALV
                     EGTLPQHRVTKLSPRPAGADDLRTLFHGAMRYW"
     gene            complement(8107..8940)
                     /locus_tag="VFV93_14805"
     CDS             complement(8107..8940)
                     /locus_tag="VFV93_14805"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5166671.1"
                     /translation="MKPRWRALAAVALAIVTAVAWLWSVILRDLTQRYTRGERPGQFP
                     GDLDADLLDDYGNVFVIAHNAGDHAETAQTALDHGADAVEIDVIMVGETLYATHAVPP
                     KLIGAWLFRGPRLADAWEIVRHADAVALDLKDSSTAYEDALVTFLSERPHDQVVLSSR
                     SIESLIRLGQRLPNAHLYLSVPNREFFDAVSATSAAFVDRLDGVSMQHSQIDADMMAT
                     LKRRDQRMIAWTVNDLTRVNELVRLGVDGITSDNLAILELLGGRERPEPLLRRRTIDQ
                     S"
     gene            9037..9756
                     /locus_tag="VFV93_14810"
     CDS             9037..9756
                     /locus_tag="VFV93_14810"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX5166672.1"
                     /translation="MIDLHTHILPGVDDGARDDAEALAMLAAAANDGITTVVATPHAH
                     HAGAERVLDGVDRLNAAARDAGTDITILPGHEARISAELATRHRDGALLTLNGTRWLL
                     LECVLFDDWPLHLIERSVDRLLAAGLRPVLAHAERYTFVQRHPDVLASLIGRGIPIQV
                     NAGSLFLRPDDIERVTAETLLRTRTAHVIASDAHNARYRPPALRAAYERAAEIAGEEY
                     AGWMRAVPQRVIAGDEVILPE"
     gene            complement(9776..11293)
                     /locus_tag="VFV93_14815"
     CDS             complement(9776..11293)
                     /locus_tag="VFV93_14815"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5166673.1"
                     /translation="MTDTETPTFGRRLAELAELHPDKAAVIFLPIAGDDQVLSWRQLD
                     LQSNQLARLLAERDVRDDSTIVVGLWNSPEHILLTIAGWKLGALVLPLRAILPKPERD
                     GIMALAKPALVCADWDDVPYPTLRPAEIARFTDYDDAPLSINPPHPGKAIASGGSTGR
                     SKIIVDRTANPTIYGRRASRLGAQPGQVQLICAPLYHNSPFLSSYGGLADDHTLVVME
                     KFDAERAVDAIERYRVNYAYMPPIIMRRIMMLPGVRERDFSSIQAIQSSAAPCPIWLK
                     QAWIDLIGAEKVHEVYGSSEGIGATMIRGDEWLEHQGSVGMPVNCELQILDENGEELP
                     AFEVGEIFMRTLNEGGPTFNYVGGAKIKAASDGFLSIGDLGYVDDDGYLYIADRRTDM
                     IVSGGANVYPAEVEAALHDHPAIGDLAVIGVPDDDWGKRVHAVIEPADPANPPSVADL
                     DAYVRERLTSYKVPKTYEFVAALPRNEAGKIRRSSLADERASGWTDRMVLARPQT"
     gene            11404..12510
                     /locus_tag="VFV93_14820"
     CDS             11404..12510
                     /locus_tag="VFV93_14820"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX5166674.1"
                     /translation="MLERFHVPREHEVRVSDAALRRTITDLFIACGVPEADAATGAGV
                     LTKTDLRGVETHGVSNMLRSYIGWFQNGQLNPDPKQHIIRETPGTATIDGDRGLGLIQ
                     GTAAMQLAIDKAREVGVGVVTMRNSGHLGAVGHFSMQAAEQDMVGICLTAMSVLVLPT
                     FGAIPRLGTNPISIAAPARTEPFLLYDAATSTIAGNKIALARRVGANMEPGWIAEPDG
                     SPIMEERPVPEGSMYALGAYNLLPLGGTREQGSHKGYGLGLMVEVLTTLLSGAVPSMV
                     EEKPPLASHHFAAYNIAAFDDVVHFKDTMDRTLQTLTETPPAPGHERVIYPGLSEAES
                     EIDRKANGIPLHREVIDWFNRTTDELGVARIETL"
     gene            12525..13610
                     /locus_tag="VFV93_14825"
     CDS             12525..13610
                     /locus_tag="VFV93_14825"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX5166675.1"
                     /translation="MLERFSVPAADQVLVDHAALRGTVTDLFVACGVAADDAAAGADV
                     LVTADLRGVETHGVSNMLRQYIGWFRDGIHNPRPEWRILHESRGTATIDGDRGLGIIQ
                     GRPAMQLAIEKARDVGVGTVTMRNSGHLGPIGHFAYQAAEQGLVGMCMTASSLLIVPT
                     FGALPRYGTNPIAFAAPARSRPFMLFDAAMSTVASNKIGLARRVGADMEPGWIAEPDG
                     TPIMDERPAPTGNFSLLPLGGTREQGSHKGYGLGLLVEVMTTLLAGAVPRMIERDTKP
                     RHCFIAYNIEAFTDPDEFKDTMDRTLQTLTETPTAPGHERVLYPGLPEYETEQHRRAN
                     GIPLHREVIDWFNTITAELSVARLETL"
     gene            13607..14824
                     /locus_tag="VFV93_14830"
     CDS             13607..14824
                     /locus_tag="VFV93_14830"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5166676.1"
                     /translation="MTGTGYTFDEMRRFHAVWSLANWDKGGPQMRYTFRRMTEFFPHV
                     LIRRAGPVSRLAESPRDEIARASVQTRRGDVSLDTYVADSSVNGVVILHRGAIVYERY
                     PRMCPDDLHLTMSVSKVFTSTLIAILEDRGLVDVLQPVDAYVPDLRGSGWEGVSVRDV
                     LDMASGIDCDQTPADVYTNPEQPYYGFEASLGWLPPLAGTADSTYDYVAGLGRLKEPG
                     EAFDYSSANTFVLAWLAEQITGRPHADLLSDEIWSRIGAEGDAAITIVPGSGATASHA
                     GLSARLRDIARFGLLFTASRERVSETRLVSDHYLRGIASGRPDIFANGITGRSMLARM
                     AGDPPSHNSYQWDYVMPDGDFYKGGYGGQGLYISPARDLVIAFAGAFEIDGPDSEMPG
                     IARQLARSVLFGP"
BASE COUNT         2558 a         4934 c         4990 g         2557 t
ORIGIN      
        1 cgctcgttga ccatctcgac ctcggcgtgc ccgtcgccgc tgccgggatc gttgcgctgc
       61 cagacctggt agcggtcacg tcccttgtag acaccgatga cgccccacga gccgtgtgag
      121 tggattggtg tcatcgcctc cggcccgaag cgcgcccgga cgagcgtcag accgccctcg
      181 tccgtgtaga gcggcgccga ctggcggccg gtgtggatat tgcctgtgaa gtcctcgtgg
      241 ctggccagca cgtccgggtc gcggacgaac gactccatcc gggttgcgat ccgttgcagg
      301 cccggttcgg ccgagccgtc ctcagccagg atcgccttga catcgctgat gaatgcgttg
      361 actgccgtcg ttaccgtcat cgtacctccc gttgtcagcg tgccgctgga gtctcgtcct
      421 tcgccggcca gccgtgcgcc agctcgtctg gcgtgtagcg cgagcgggtc agtggtccgg
      481 ccggccgcag gcgtagccgg cggcggttgg ggtcgatttc cggatcgacc tgctcgacgg
      541 tgaccggccc gtgccacggc tcccagacgc cgccgtatgg gcggaagcgg atgtagagct
      601 cgtcgcccca gcctgctgat gcgtcgtact cgtcggtcgt gacggcgatc tcgccgcgcc
      661 agtcgtccag cggcagtgca ttgccgacca tctcgtacgt caccagacac cagccgcgcg
      721 ccagctcctc gccgttctca ccgcgataga tggtccccgg caacgtctgc gtatcaaccg
      781 gcatcgatct gctcccttgt caacgatcgg cgtcaagccg ccagcgtgat ggtcgcacta
      841 tgatagcgcg gctgcgcaca tctgcgacgt cggcagtcgt gacttctgtt acctctagca
      901 gggatttgcc ggctggttcg gtggttcagt gtatatcgtc gagcggccag aagctgaaga
      961 ggacgtcgcc gaggacggcg tcgcggtcga ccgggccgaa gacgcgcgag tcgctggagt
     1021 ttggccggtt gtcgccgagg acatagatca tcccggcggg gacggtcagc gagcagtacg
     1081 gcgcgtggaa gcagtccgtc tcggcgccgt tgatgtacgg ctcgtcgagc cggatcccgt
     1141 tgcggtagac ggcgccgtcg tggatgtcga tcgtgtcgcc ggccagcccg atgacgcgct
     1201 tgatatacgg cttgtcgctc tcgatcggcg ggttgaagac gacgacatcg ccgtggtggg
     1261 ccgactggaa tcgataggca ccgttgagaa tgacgcgtcc gccgtcgtgc aggcttggcc
     1321 acatgctctc gccctcgatg gcgtacgacg gtgcgacgac ccgcagtccg agccagatcg
     1381 ccacgcagag cagcaggaag cgcagcgtcg gtagccagtt gcgccggaac gagccgtctg
     1441 gccgttctgc ctcatccagc tgggtcggtt ctgcgttcat tgctgcattg tgctcgccgg
     1501 ctgctgccat ccagctggcc ctcccgggac atgacatttg tcacggcagc ataccgagtc
     1561 gaagcaggtg gctgccagtc gttgagcgta gcacgcttcg tgcgccaccg ttttgtcatc
     1621 ctgaggtgtg agccgaagga tgacaagtca gcgtttgatg atgccacgca ggaggaagag
     1681 gacgttggcc ggacgttcgg cgaggcggcg catgaggtag aaataccact cgctgccgta
     1741 tggcgtcgcg accagcacct tgtagccccg gctggcgagc tcggtctggt agcggctgcg
     1801 aatgccgtag agcatctgga actcgaaccg gtcgcgcgcg atgccgttgc ggccggtgaa
     1861 gtcgatgacg tgctcgatga tccgttcgtc gtgggtcgcg attgcagtgt agccgtcgcc
     1921 ggccagcatc cgctcggcca gccgaatgta gttgcggtca acatcggcct tctgcgggta
     1981 agcgaccgac gccggttcaa ggtaggcgcc cttgacgagc cgcaggttgg gcgcgagcgg
     2041 gagcagcgct tccagatctc gttccgagcg gtagagcgcc gattgcagga cggttccgac
     2101 gttgacgtgc ccggcatcgc gcagccgacg gtagatgcgc agcgtcgcat cgacgcgggc
     2161 cgactcctcc atgtcgatcc ggatgaagtt gccgagcgtt gcggcgtggc tcacgatctg
     2221 ttcgagatta gcgtaggcgg tctcctcacc cagatcgagg ccgaggtggg acagcttgag
     2281 cgcgatgttg gtttgcagcc cttccgccgc gagcctgtcg agcaacgctt tgtaggcagt
     2341 gacgacggca gccgctgccg cggcatcgcg cacgccctcg cccagtagcg tcgtgttggt
     2401 caccaggccg cgctgattga gcgaccgcag gactggtacg gcggcgtcca gcgactcgcc
     2461 ggcgacgaag cgagagccac ccagccgcaa gccgtagcga cggacgatcc gttcgacgag
     2521 ccggttgtgg gttgccagca ggatcagctt gcggaagatc gggttaaacg agaacgaccg
     2581 gcgcgcgggg cgctcgagcg tgcccacgct actcccctcc acagcggcct cccttctgac
     2641 cgaacggcat tccataccgc gcatcgtgcc agcgattggc cgtcagacca acgactgcca
     2701 cgtacaatgg tgcgacgcgc ggcgctgcct catcgcgcat gaggatttga catcagcgat
     2761 ggttgaatgt ccagcgacac gaccggaagg gtttacgacc gagacgtcag cacctagcga
     2821 catgggacgc gcatgcaacg actgtacgtg tcacgatcgc aacgacgcgg cgccgaagtc
     2881 cgtatcatcc tgctggccgt ctggctgatg gtggcgaccc tgttgaccgc ctgtagcggc
     2941 cccgaatcgg gccaggacgc gcggacgacg ccgatggcga cgaggactgc tacgtccgtc
     3001 agcggcgtgc cgaccagcac gagtgccgcg accgagacag ccaccgcgac gatcccggcc
     3061 gtcgttgaga cccccggcga gccgacagcg acggtgcagg ctggaactcc aactgaaccg
     3121 ccgaatccga ctgcgacggc aacgcccatt acgccgacgc aggagctgac gccgacgcca
     3181 cagccgccga tcgacctggg gcaggttcag atctcagtcg agcaggtcgg ctccgggttc
     3241 agccagccgg tgacgctgac ccacgccggt gacggcagcg ggcggcgctt cgtcgtggag
     3301 aagaccggca tgatccgcct tctggatggc agcgtctacc tcgatctgac ggaccgtgtc
     3361 ctcgcctacg atgtccgaac gacgcagcat gagcttggtc tggtcggcct ggcgtttcat
     3421 cccgacttcg agacgaatca ctacttctac gttcactaca cgaatcagga ccaggaccac
     3481 gtcatctcgc gcttcagcga gggcgccgac gggctcggcg atccggccag cgagaaggtt
     3541 ctgattacct acgaccagcc ggacgtgaac ttcttcggtg ggacgctggc cttcgggccg
     3601 gatggctacc tctacatcgg gatgggaacc gggacgtcgg ttgacgccga ccagatcgtc
     3661 gcgcagcagc tcgacaacct gtatggcaag atcctgcgga tcgacgtcaa cagtggcgac
     3721 ccgtatggaa tcccgccgga caacccgttc gtcaacactc cgggtgcgcg cccggaggtc
     3781 tgggcgtatg gcctgcgcaa cccctggcgc ttctcgttcg atcccgccaa cggcgatgtc
     3841 tacatcggca gccccggcga gttcgcgcgc gagtggatca actacattcc ggccggctcc
     3901 gcgccgggga agaacttcgg ctggccgatc ctcgaaggag gcgagtgctg ggaatactgg
     3961 accggcgact gcacgaccga aggattcgag ctgcccatct acgtccggcc aacctatcag
     4021 gacggcaact gcgtcatcct cggcggcttc gtctaccggg gccagctgtc gccgtcgctc
     4081 aacggcgtct acctcttcag cgactattgc agcggccgga tctggggtct ggcgcgcgac
     4141 gccgccggcg tatggcagag cgcagaactc tacaacctcg cggcgctggt ctcgtcgttc
     4201 ggcgaagacg aagcgggcga gatctatatc ttcgagattg agaatggcat cgtgtacagg
     4261 atcgtaggta ctaggtgata ggccataggt gttaggggtt agtcggtgag gatcagcgcg
     4321 ctgcgttcct cctgaacgaa ccagtcccta acacctatgg cctatcacct agtacctccg
     4381 tcacgtcctc cagccgccca ggttcgtcgt gagctggtcg atgtcggttg ggcggcgcag
     4441 gaagtgcagg ctgatggtgt cgttgctgcc gtcgggccag gggccaggga ccgtctgggt
     4501 gtcgccgtgc caaccgaggg ctgcggccgt gttgaggaag cgctgggcgg tcttgcggcc
     4561 cggctcggcc agccagagca tgccgtccgg cgcgagaatc cgctcgacga tcgccagcag
     4621 cggctcgaca tcgcgcccct cgtagaggac atcagccgcc agaatcagcg ggtatcctcc
     4681 cccagatgcg gcgcgggcga cggctccgtc atccggcgtg cgccagttga agcgcagcgt
     4741 tcgcggcgag cggccggtgt tggagagcgc gttgtagcgg cagaacgcca gcggcagcgg
     4801 cgagtagtcg ctgaccgtca gaatcgcgcc ggcttccagc agcgtcgagg ccgtcgtccc
     4861 aagcccgcag ccgagttcca gcacgcgctg gccggcgagt tcggcgcgcc gttcgagcgc
     4921 gatatcagcc agagcgacgc cgctgggcca gatccgtgcc cagtagggca tctgcttgtc
     4981 gggatcgagc ctggcggcct cgaacatccg ctcgcgggct gcctcagtcg gcttgcggat
     5041 cgcaagcttg cggccgctgg ccggcagaat gatctgctgc gtctctagct cggcctcggc
     5101 ggacaggtag tcggccagca cgtgcaggcg gcgtgcgttg ctggtggaac cggcgccgat
     5161 gcgatcgtcg gtcagttcgt cgcaggccgg ctggtcgagc acgttatcca ccgttcatcg
     5221 gtccagccat ccgtcccgca tcacgaccgt gccgtccggc gggtctccag cgtcatccca
     5281 tcggaggcga gatagaggat tcccatcagc aacgcgcaga cgacgccaac ggccagcatg
     5341 acatcggtgc tgtcccagaa gcgcgccatc aatgcgacag cgatcgcggc cacgatctga
     5401 ccggcgatga cggcgacgag cattagtcga ccagcccctg gcaggtcggt ttccggcgcg
     5461 atacgacgta tcgccgtcgc cgtcaccagg tcgttcgtga tcgagatgaa gccgatcccc
     5521 agcaagacga ggacgacgat ctcgatccgg gtgacgatac tcatcaggat gatcccggcc
     5581 gcaatcccga tcgctgagag ggtgacgatc agtgagaccg gcaggtggcc cagcagacgt
     5641 ggcacggaca gtggccccag cagcgtaccg gcgccgacca gcgccagcag cagccccagc
     5701 gtttcgtctg agcggttcag cggttcggcc gccagctcga tcaggacaac cttcaggcaa
     5761 atcgccagcg ccgacccgat gaacagcccg ctcgccagta gcagcaggcc gggttggtgc
     5821 cgggcaacga tgaggactga ttcgtcattg tgcaggctgg acgccggctg cattgctgga
     5881 tcaatcgcga ccggcggctg gagccagagc agaacgctcg ccaccagcac cagaccggcg
     5941 ctggccagca gcgcgacctc gatatcccag ccccgcagga tcgtcgctgc cagaagcggg
     6001 ccggcgacga aggtgatgcg ttcaatcgat gcattcagat gggtcgccag ggccagtcgc
     6061 gtccggggga cgattcgcgg gagcgttgcc gcgcgtgact cgttcgagag cgccgtcagc
     6121 agcccataca tgacgccgac tacgcccgcc cagaccagat ctgcgcgact gtcaagcaac
     6181 gccaggctgg cgatcagcgg cacgcgcgac aggccgagca ggaaccacca gtcgggactg
     6241 atgccggttg ccggtttgtg aaagcccagg ctgatgacgg cgcgcggcag aacgcgtagt
     6301 agcatgaaca acgcgacgac cgcgatgtca gccgtgacgg catagacggc aacgaacagc
     6361 gcgacgagca ggacccagtc ggcgagcagt tcaagcgtgc gtgccgacca gaacgagagc
     6421 gcgcgaccat cgcctgcgag gtatcgaaac ggacggaaca atccgagcag ggctgtcgag
     6481 tcgaacacgg acaggctcct tacccagcac cagtcgcttg ccggattcgc cgttgttgca
     6541 agaaggataa catcgggcgc gatcgcgaac cctgatagct cgctgacaac cgatctcgca
     6601 agtcgcctgc cgcaccgttg tcattcatgc tggtgacacg cgctcgattt cacggtacgg
     6661 tcctggctgg atgcggacca gacactcatc cgaaaggttg gcgcgatgac gactgaagcg
     6721 atcttcacga tggatacttc gagcatcaag tacggggccg gcgcgacggc ggagattggt
     6781 ttcgagatgg cgcggctcgg cgccggccgg gtgctggtcg tgaccgatcc caacctgcgt
     6841 gacagcgacg cggtcggcaa ggcgctcgcc tcgctcagtg ccgccggcat cgagtcggtc
     6901 gtgtatgacc gcgttcgagt cgagccgact gacgtgtcgt ttcgcgaggc gatcgagttc
     6961 gcgagcgatg gcggcttcga tggctatgtc ggcattggcg gcggatcagc gatcgacacg
     7021 gccaaggtcg ccaacctctt cgcgacctgg ccggctgatc tgctgacgta tgtcaacgcg
     7081 ccgatcggcc aggcgaagcc ggttcccggc ccactcaagc cgctgattgc catcccgacg
     7141 acggcgggga ccggcagcga gacgaccggc gtcgcgatct tcgacctgac cgagatgcac
     7201 gccaagacag gcatcgccca ccgcgcgctg cggccggtga tggggatcgt cgatccggac
     7261 aacacgcgct cgatgccgcc gatggtggcc gcctgctcgg gctttgacct cctctgtcac
     7321 gcgatcgagt cgctgacggc gctgccgtat tccgaacggc ccgcgccgga gcatccgtcc
     7381 gtccgtccgg cctatcaagg cgccaacccg atctccgaca tctgggcctc gaaggcgctg
     7441 gagctgacgg ccgccaacat cgtccgggcc gtccagaacc cggacgacga tgcggcccgc
     7501 gctgcgatgc tgctggcggc cacctacgcc gggatcggct ttggcaatgc cggcgttcac
     7561 ctgccgcacg ggatgtcgta tcccgtctcg ggaatggtgc gcgacttccg accggagggg
     7621 tatcaggtcg atcacgcgat catcccgcac ggcatgtcgg tcattctcaa cgcgccggcc
     7681 gtcttccgct ggacggcttc tgccaatccc gagcgtcatc tctatgccgc cggcttgctc
     7741 ggcgcggaga cgcacggcgt caatccggac gaggctggcg agttgctggc gacgaccatc
     7801 atcgacctga tgcgtgcgac cgggatgccg aacggcctcg ccgccgtcgg cttcgaaggt
     7861 gacgatatcg acgcgctggt cgaagggacg ctgccgcagc accgtgtcac caagctctcg
     7921 ccccgccccg ctggcgccga cgacctcaga accctgtttc atggcgcgat gcgctactgg
     7981 taatagtgat cgccgaccga ataggcaatg ggcaatccgt agggcgatgt cggtgactcg
     8041 ccctcaatac ccaatgacca atggcgtatt ggacgattga aattgggcga gtcaccaaca
     8101 tcgcccctac gattggtcaa ttgttcgacg acgtagcagc ggctccggtc gctcccggcc
     8161 gccgaggagt tccaggatcg cgagattgtc gctggtgatg ccgtcgacgc cgagccggac
     8221 cagctcgttg acgcgggtga ggtcgttgac ggtccacgcg atcatgcgct ggtcacggcg
     8281 cttcaaggtc gccatcatgt cagcgtcgat ctggctgtgc tgcatcgaga cgccgtcgag
     8341 ccggtctacg aatgctgctg atgtcgcaga gacggcatcg aagaactcgc gatttgggac
     8401 gctgagatac agatgcgcgt tcggcagtcg ctggcccagg cggatcaggc tctcgatact
     8461 gcggctgctg agcacgacct ggtcatgtgg acgctcgctc aggaacgtta ccagtgcatc
     8521 ctcgtaggcg gttgacgagt ctttcaggtc gagtgctacc gcgtcggcgt gcctgacgat
     8581 ctcccaggca tctgccagtc gtggaccgcg aaagagccag gcgccgatca gctttggtgg
     8641 caccgcgtgg gttgcgtaca acgtctcacc gaccatgatg acgtcgatct cgaccgcatc
     8701 cgcgccgtgg tcgagcgctg tctgggccgt ctcagcgtgg tcgccggcgt tgtgggcgat
     8761 gacgaagacg ttgccgtagt cgtcgagcag atcggcatcg aggtcgcctg ggaactggcc
     8821 gggccgctct ccgcgcgtgt accgttgtgt gagatcgcgc aggatgaccg accagagcca
     8881 ggcgacggct gtcacgatcg ccagcgcaac cgccgccagt gccctccatc gtggcttcat
     8941 ctcatccggc tcccgtcccc gtcgcaacac gcgacctgca gagtatggca tctggccctg
     9001 agagaggact gcgcgccgga cgtgaacgat tgctcgttga tcgatttgca cacccatatc
     9061 ctgccgggcg ttgacgatgg tgcgcgggac gatgccgagg cgctggcgat gctggctgcc
     9121 gccgcgaacg acggtatcac gaccgtcgtt gcgactcccc acgcccatca tgccggcgct
     9181 gaacgggtgc tggacggtgt cgaccggctg aacgcggcgg cgcgcgatgc cgggaccgat
     9241 atcacgatcc tgccgggtca cgaggcacgc atcagcgccg agcttgccac tcgccatcgt
     9301 gacggcgcgt tgctgacatt gaacggcacg cgttggttgc tgctggagtg cgtcctgttc
     9361 gatgactggc cgctgcacct gatcgagcgg tcggtcgatc ggctgctggc tgccggcctc
     9421 cggcccgtgc tggcgcacgc tgagcgctat acgttcgtcc agcggcatcc ggacgttctc
     9481 gcctcgctga tcggtcgcgg tatcccaatc caggtcaacg ccggttcgct cttcctccgg
     9541 cccgacgaca tcgaacgcgt gacggccgaa acgctgctgc ggacccggac agctcacgtc
     9601 atcgccagcg acgcccacaa cgcgcgctat cgcccgcccg cgctgcgggc cgcgtacgag
     9661 cgcgcggccg agatcgccgg tgaggagtac gctggctgga tgcgggccgt gccgcagcgg
     9721 gtgatcgccg gcgacgaggt catcctgccg gagtagcggg tgtccgcaaa cccgctcacg
     9781 tctgtggtcg tgccagcacc atcctatccg tccagccgct ggcgcgctcg tcggccagcg
     9841 acgagcggcg gatcttgccg gcttcgttgc ggggcagggc ggcgacgaac tcatacgtct
     9901 tgggtacctt gtacgacgtc agccgctcgc ggacgtaggc gtccaggtcg gcgacggacg
     9961 gcgggttggc cgggtcggcc ggctcgatga cggcgtgaac ccgtttgccc cagtcgtcgt
    10021 ccgggacgcc gatgacggcc agatcaccga tggcggggtg gtcgtggagg gcggcctcga
    10081 cctcggccgg gtagacgttc gcgccgccac tgacgatcat gtccgtccgc cggtcggcaa
    10141 tgtagaggta gccgtcgtcg tcgacgtagc caaggtcgcc gatgctcagg aagccgtcgc
    10201 tcgccgcctt gatcttcgcg ccgccgacat agttgaaggt cgggccgccc tcgttgagcg
    10261 tccgcatgaa gatctcaccg acctcgaagg caggtagctc ttcaccgttc tcgtccagaa
    10321 tctgcagttc gcaattgacc ggcatgccga cgctgccctg atgctccagc cactcgtcgc
    10381 cacggatcat cgtcgcgcca atgccctccg acgagccata gacctcgtgg accttctcgg
    10441 cgccgatcag gtcgatccag gcctgcttga gccagatcgg gcagggcgcg gccgacgact
    10501 ggattgcctg aatactggag aagtcgcgct cgcggacgcc gggcagcatc atgatgcgcc
    10561 gcatgatgat cggcggcatg taggcgtagt tcacacggta gcgttcgatc gcatcgaccg
    10621 cacgctccgc gtcgaacttc tccatcacaa ccagcgtgtg atcgtcggcc aacccgccgt
    10681 agctgctcag aaacggggag ttgtgataga gcggcgcgca tatcagctgg acctgacccg
    10741 gctgggcgcc gagccgcgag gcgcgccgtc cgtagatcgt cggattcgcc gtccggtcga
    10801 cgatgatctt cgagcgcccg gtcgaacctc cggaggcgat cgccttgccg gggtggggcg
    10861 ggttgatcga cagtggcgcg tcatcgtagt ccgtgaagcg cgcgatctcg gctggtcgga
    10921 gcgtcgggta cgggacgtcg tcccaatccg cgcagacgag tgccggtttg gccagtgcca
    10981 tgatgccgtc ccgctccggc ttcggcagga tggcccgcaa cggcaggacc agcgcaccca
    11041 gcttccagcc ggcgatcgtc agcaggatgt gttcgggcga gttccagagg ccgacgacga
    11101 tcgtcgagtc gtcgcggacg tcgcgttcgg ccagtagccg cgccagctgg ttcgactgca
    11161 gatcgagttg acgccaactg aggacctgat cgtcacccgc gatcggcagg aagatgacgg
    11221 ccgccttgtc agggtgcagc tcggccagct cagcgagccg ccggccgaag gtaggtgtct
    11281 ccgtgtctgt catatcgccc ctcgcctgtt ccaatcccct gcgaaccggc catatactac
    11341 gccgacctgc cattgttgac gacaggcgcg acgataacgg ccaacggtct gggaggatga
    11401 cggatgctgg aacgatttca tgtcccgcgc gaacacgagg tgcgggtgag cgacgccgcg
    11461 ttgcgccgga ccatcaccga cctgttcatc gcctgcggcg tccccgaggc ggacgccgcg
    11521 accggcgccg gcgtgctgac gaagaccgat ctgcgcggcg tcgagacgca cggcgtctcc
    11581 aacatgctgc gctcctatat cggttggttc cagaacgggc agctgaatcc tgatccgaag
    11641 caacacatca tccgcgagac gcccgggacg gcgacgatcg acggcgaccg agggctgggc
    11701 ctgatccagg gtacggccgc catgcagctg gcgatcgaca aggcccgcga ggtcggcgtt
    11761 ggcgtcgtca cgatgcgcaa cagcggccat ctcggcgcgg ttggacactt ctcgatgcag
    11821 gctgccgagc aggacatggt cggcatctgc ctgacggcga tgagtgtcct tgtcctgccg
    11881 accttcggcg cgatcccccg actgggtacc aacccgatct cgatcgccgc gccggccagg
    11941 acggagccgt tcctgctcta cgacgcggcc acctcgacga tcgccggcaa caagatcgcg
    12001 ctggcccgcc gggtcggcgc caacatggag ccaggctgga tcgccgagcc ggacggttcg
    12061 ccgatcatgg aggagcgacc agttccggaa ggctcgatgt acgccctcgg tgcctacaac
    12121 ctgctgccgc tgggcgggac gcgcgagcag ggatcgcaca agggctacgg gctgggcctg
    12181 atggtcgagg tcctgacgac gctgctgagt ggggcggtgc caagcatggt cgaggagaag
    12241 ccgccgctgg ccagccacca cttcgccgcc tacaacatcg ccgcctttga cgatgtcgtg
    12301 cacttcaagg acacgatgga ccgcacgctc cagacgctga ctgagacgcc cccggcgccg
    12361 ggccacgagc gcgtcatcta tccgggtctc tcggaagcgg aatcggagat cgatcgcaaa
    12421 gccaacggca tcccgctgca ccgcgaggtc atcgactggt tcaacaggac gaccgacgag
    12481 ctgggtgtcg cccggatcga aacgctgtaa cggaacgggg cagcatgcta gagcgattca
    12541 gcgtaccggc cgccgatcag gtccttgtcg atcacgcggc gctgcgggga accgtcaccg
    12601 atctgttcgt cgcctgcggc gtggctgccg atgacgcggc ggccggcgcg gatgtgctgg
    12661 tgacagccga cttgcgcggt gttgagacgc atggtgtctc gaacatgctg cgccagtaca
    12721 tcggctggtt ccgcgacggc atccacaatc cccggccaga gtggcgaatt ctccacgaat
    12781 cgcgcgggac ggcgacgatc gacggcgacc gggggttagg catcatccag ggccggcccg
    12841 ccatgcagct ggcgatcgag aaggcgcgcg acgtcggcgt cgggaccgtc acgatgcgta
    12901 acagcggtca tctcggcccg atcggccact tcgcctacca ggcggctgaa cagggactcg
    12961 tcgggatgtg catgaccgcc agcagcctgc tgatcgtgcc gaccttcggc gcgctgcctc
    13021 gctacggcac caacccgatc gccttcgcgg cgccggcccg ctcacgcccg ttcatgctct
    13081 tcgatgccgc gatgtcgacc gttgccagca acaagatcgg cctggcgcgt cgcgttgggg
    13141 ccgacatgga accgggctgg atcgccgagc cggacggcac accgatcatg gacgagcggc
    13201 cggcgccgac cgggaacttc tcgctgctgc cgctcggcgg cacccgtgag cagggctccc
    13261 acaagggcta tggcctcggg ctgctggtcg aggtgatgac gacgctgctg gcgggcgcgg
    13321 tgccacggat gatcgagcgc gacacgaaac cgcgtcactg cttcatcgcc tacaacatcg
    13381 aggcgttcac ggacccggat gagttcaagg acacgatgga ccgcacgctt cagacgctga
    13441 cggagacgcc aaccgcgccg ggccacgagc gagtcctcta tcccggcctg cccgagtacg
    13501 agacggagca gcaccggcgc gcgaacggca tcccgctcca tcgtgaggtc atcgactggt
    13561 tcaacacgat caccgccgag ctgtcggtcg cccggttgga gacactgtga ccggcacggg
    13621 ctacacgttc gacgagatgc gccgattcca cgccgtctgg tcgctcgcca actgggacaa
    13681 gggcggcccg cagatgcgct acaccttccg gcgcatgacc gagtttttcc cgcacgtgct
    13741 gattcgccgc gcaggtccgg tttcccggct ggcggagtcg ccgcgcgatg agatagcacg
    13801 cgccagtgtc cagacacgcc gcggagacgt gtcgctcgac acctacgttg cggacagctc
    13861 agtcaacggg gtcgtcatcc tgcatcgcgg cgcgatcgtc tacgagcgct atccacggat
    13921 gtgcccggac gatctgcacc tgacgatgtc ggtctcgaag gtcttcacca gcacgctgat
    13981 cgcgatcctc gaagatcgtg gactggtgga cgtgctccag ccggtcgatg cctacgtacc
    14041 cgatctacgt ggctccggct gggaaggcgt cagcgtccgc gacgtgctgg acatggcctc
    14101 cggtatcgac tgcgaccaga cccctgccga tgtttacacg aacccggagc agccctacta
    14161 cggcttcgag gccagtctcg gctggcttcc gccgctggcc gggacggcag attcgaccta
    14221 cgattacgtc gcgggacttg ggcggctcaa agagccaggg gaggcgtttg actactcctc
    14281 agccaacacg tttgtgctgg cctggcttgc ggaacagatc accggccgcc cacatgccga
    14341 cctgctcagc gacgagatct ggagccggat cggcgccgag ggcgatgccg ccattacgat
    14401 cgtgcccggc tccggcgcga ccgcctccca cgccggcctt tcggcccgcc tgcgcgacat
    14461 cgcccgcttc ggtctgctgt tcaccgccag ccgcgagcgc gtcagcgaga cgcggctcgt
    14521 ctccgatcac tacctgcgcg gaatcgccag cgggcggcct gatatcttcg ccaacggcat
    14581 caccggccgg tccatgctgg cccggatggc cggcgaccca cccagccaca acagctacca
    14641 gtgggattat gtcatgccag atggcgactt ctacaagggc ggttacggcg gtcaggggct
    14701 ctacatctcc cccgcccgcg atctcgtcat cgccttcgcc ggcgcgttcg agatcgacgg
    14761 ccccgacagc gagatgcctg gcatcgcacg ccaactcgcg cgctcggtct tgttcggccc
    14821 gtgatcgccg gaaccggcgt caaacgctcc ccctgtcgtc ctgagccgca gcgaaggacc
    14881 tccctgcttg aacgccatac gagaccaaag cggagaggtc cttcgctgcg gctcaggacg
    14941 acagggggag cgtttgacgc cggttccggc gatcacgggc cgaacaagac cgagcgcgcg
    15001 agttggcgtg cgatgccagg catctcgctg tcggggccg
//
LOCUS       DASUGJ010000650         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_801061, whole genome shotgun sequence.
ACCESSION   DASUGJ010000650 DASUGJ010000000
VERSION     DASUGJ010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801061"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..2031)
                     /locus_tag="VFV93_14835"
     CDS             complement(4..2031)
                     /locus_tag="VFV93_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX5166677.1"
                     /translation="MTVDAAVHAKPATIRRDEAATTAEHTIERDYRRVVIEGVSPEID
                     GGRFPMKRTVGEPVVVEADAFADGHDAITCVLLYRRESASEWSVVTMTPLGNDRWRAK
                     FIPESLGRYRYAVEAWVDPFKTWSGDLDKRVEAGQDVAVDLQIGAALVDAAAERASGR
                     DKADLKQFAAALAAGGAVAVRQALSPVLAMLVERYPDRSAATVYDPGLAVVVDPERAR
                     FSTWYELFPRSTSPEPDRHGTFRDVEARLPVLADMGFDVLYLPPIHPIGSTFRKGKNN
                     ALVALEDDTGSPWAIGSPEGGHTAIHRELGTLDDFRHLVATAREAGIDLALDIAFQTS
                     PDHPYVREHPAWFRHRPDGTIQYAENPPKKYQDIYPFNFESEDRQSLWDELKGVFLYW
                     IEQGVTIFRVDNPHTKPFRFWEWLIAEVKRDHPQVIFLAEAFTRPKVMKHLAKLGFTQ
                     SYTYFAWRNARWELVEYLTELTQTDVREYFRPNFWTNTPDILTEYLQTGGRPAFITRL
                     MLAATMTASYGMYGPAFELGEYQPREHGSEEYLNSEKYEIRRWDLDRPESLRELIARV
                     NRIRRANPALQFNDRLRFHHVDNEQLVCYSKSTADFGNVILVVVNLDPRNTQSGWVDC
                     DLGSLGLNWDDRFQVYDLLANAYYTWQGPRNYVELNPHVIPAHIFQVQRQT"
     gene            complement(2602..>2797)
                     /locus_tag="VFV93_14840"
     CDS             complement(2602..>2797)
                     /locus_tag="VFV93_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166678.1"
                     /translation="GLTEGALIASDLGRVVIGGLFSSTLLTLFVVPVAYSLADGLRQR
                     FSRRRRSTELRGAEPVPVGD"
BASE COUNT          495 a          886 c          910 g          506 t
ORIGIN      
        1 tggtcacgtc tgccgctgga cctggaagat gtgggcgggg atgacatggg gattcagctc
       61 gacgtagttg cgtggcccct gccaggtata gtacgcgttg gcgagcaggt catagacctg
      121 gaaccgatca tcccaattca gcccgaggct gccgaggtcg caatcgaccc aaccagactg
      181 cgtattgcgc gggtcgaggt tgacgacgac gaggatcaca ttgccgaaat cagccgtgct
      241 cttgctgtag cagaccagct gctcgttgtc gacgtgatga aagcgcagcc ggtcgttgaa
      301 ctgcaacgcc ggattcgccc gccggatgcg gttgacgcgg gcgatcaact cgcgcaggct
      361 ctcgggccgg tcgagatccc atctgcggat ctcgtacttt tccgagttca ggtattcctc
      421 gctgccgtgc tcgcgcggct gatactcgcc cagctcgaac gctggtccat acataccgta
      481 actggccgtc atcgtcgccg ccagcatcaa ccgggtgata aaggcgggcc gtccgccggt
      541 ctggagatat tcggtgagga tatcgggcgt gttcgtccag aagttcggcc ggaagtactc
      601 gcgcacatcg gtctgggtca actcggtcag gtactcgacc agttcccatc gggcgttgcg
      661 ccaggcgaaa tacgtgtagg actgggtgaa gcccagcttg gcgaggtgct tcatcacctt
      721 cggccgggtg aacgcctcgg cgagaaagat cacctgcgga tggtcgcgct tgacctcggc
      781 gatcagccac tcccagaagc ggaacggctt cgtgtgcggg ttgtcgacgc ggaagatggt
      841 gacaccctgc tcgatccagt agaggaagac gcctttcaac tcatcccaga gcgattgccg
      901 gtcctcgctt tcgaagttga aaggatagat gtcctggtac ttcttcggtg ggttctcggc
      961 gtactggatc gtgccgtccg gccggtggcg gaaccaggcg ggatgctcgc gcacgtaggg
     1021 atggtcgggc gacgtctgaa aggcgatgtc cagcgccagg tcgatgccgg cctcgcgggc
     1081 ggtcgcgaca agatgacgga agtcatcgag cgtgccgagt tcccggtgga tcgccgtgtg
     1141 gccgccctcg ggcgagccga tcgcccaggg gctgcctgta tcatcctcca gcgcgacgag
     1201 ggcgttgttc tttcccttgc ggaaggtgga cccgattggg tggatcggcg gcaggtagag
     1261 cacgtcgaag cccatatcgg ccaggaccgg cagccgtgcc tcgacatcgc ggaaggtgcc
     1321 gtggcggtcc ggctccggcg aggtcgagcg cgggaacagc tcgtaccagg tgctgaaccg
     1381 ggcgcgctcc ggatcgacga cgacggcaag accggggtcg tagacggttg ctgctgaccg
     1441 gtccggatat cgctctacca gcatcgcgag caccggcgag agcgcctgcc gcacggcgac
     1501 ggcgccgccg gccgcaagcg ccgccgcgaa ctgcttcagg tcagccttgt cccgaccgga
     1561 cgcgcgttcc gcggccgcat cgacgagcgc ggcgccgatc tggaggtcaa ccgccacgtc
     1621 ctgcccggcc tccacccgtt tgtccaggtc accggaccag gtcttgaacg gatcgaccca
     1681 tgcctcgacc gcgtagcgat atcgcccaag cgattcagga atgaacttag cccgccagcg
     1741 gtcgttgccc agcggcgtca tcgtcaccac cgaccactcc gaggcagact ctcgtcggta
     1801 gaggagcacg caggtgatcg cgtcgtgacc gtcggcgaag gcatcagcct cgaccacgac
     1861 cggttcaccc acggtccgct tcattgggaa gcgaccacca tcgatctccg gactgacgcc
     1921 ctcgatcacg acgcggcgat aatcccgttc aatcgtgtgc tctgccgtcg tcgcagcttc
     1981 gtcgcggcgg atggttgccg gcttcgcgtg tactgcagcg tcgacggtca caatcttctc
     2041 catgcacagc ttcttggcgt gcgtcgtggt atcgcgaggc agaagagcgg ccccgctctc
     2101 gatctacctt agcgcgacga tgagagagta tgcatcatgg cgcgactcga cgcccggcct
     2161 ttcgggcatg gtggcgcgat gcgtgagcgc gaaatcgctt cagcgcggac tcccgctcat
     2221 ctgcacgcaa acgatagcca cactggagac aggctatgtc cggcgggccg ccggcaaggt
     2281 ctggttcgag ccgaaggtcg ccaccacatt tggggcagga tttgtatagc atcgttaacc
     2341 gtgtcctacg tgaactcggt ccatcatgcc cccaggccgc gggccgcggc aggaccggaa
     2401 ggcgtatccg cagtcaccca attcgggggg ttgcgcgcat tccaacggcc ggatgcagcg
     2461 ccgacgactc tgcatcggtc gcccgcgtcc ctcatgtcat cctgaacgaa gtgaagcatc
     2521 tcggaggctt gactcttgtc cgagatgctt cacttcgttc aggatgacaa cgggttctgt
     2581 caccgttgac tcacaggcac cctagtcgcc gaccggcacc ggctctgctc cacgcagttc
     2641 ggtggaccgc cggcgacggg agaagcgttg ccgcaggccg tcggcgaggc tgtaggcgac
     2701 cggcacgacg aacagcgtca gcagcgtcga gctgaacagg ccaccgatca cgacccggcc
     2761 caggtcggag gcgatcaacg ccccctcggt caggccg
//
LOCUS       DASUGJ010000651         7143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1774360, whole genome shotgun sequence.
ACCESSION   DASUGJ010000651 DASUGJ010000000
VERSION     DASUGJ010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774360"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..825
                     /locus_tag="VFV93_14845"
     CDS             439..825
                     /locus_tag="VFV93_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166679.1"
                     /translation="MPLKLHLFEAPREVIRPLNVIWTPTLLVADRRLAVHYRSLNFLP
                     PREFLTMLDIGEAEHWLRWSKSEQAIALLERAEQRDPTGPLAAEAIYRRGIATYLLTH
                     SNPEMYTVWQTLLDRFPESEWAKRVP"
     gene            complement(886..3219)
                     /locus_tag="VFV93_14850"
     CDS             complement(886..3219)
                     /locus_tag="VFV93_14850"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEX5166680.1"
                     /translation="MDISAGELLQGYDLSSLQQMAKAQGIKVGSGGKYIVVPTLAALL
                     FDAGRIKRMLGDLSDAERALLDHVILAGGEAPTTLLQNQLEGEGITDKPTKTTHYGYS
                     YYQREKGSYRNRNSRKFEDLVARLGALGLLFTLDPVRSSGYIVSITEPGKRLIIPDPI
                     LEQLPEVELEVETVAEPETVQTSDAGQLLRELYLLFSLADREKIALTNRGLVMKRSLT
                     KIGQVLGREKEVAAVKTEEQLAGFSLLRGLAEELGLLAGSVGELLLDPRGEQFLALPS
                     AERRQRLFDAYRTTARWSELHRVPDLGVRGKGLNIYHAPANVPPARQRVLAELSELPA
                     GEWIPIEHLLTRLRLRAYEFLFSRTPSPNSYYAVNYRYYGFGHYNPYVANVNGLGVTL
                     DIENERSGWDIAETSFIRVVVTEALYALGVVDLGGKGDATTVFRITDDGRRLLAGEPL
                     VEDETAAQVIVQPNFQVFAFQPTGEDVLFMLDRIAERIRTGQAMEYELTQASMYRAQR
                     GGLDTDEVIRFLESVSTHELPQNVRRTLEEWGAQHERVTLRKRTPLVQTVDEATLDAL
                     YADESIGPLLGRRMAATLALVPADQLQTVFRSLTANGQLVALSEGPGFQVGPQLDVEP
                     DGTLAFRQPQPAIYTLHELQPFVDAENGQLRITPDSLRRSARNGLDADAVVATLERLT
                     GGTLPGDPAALVRRWARDWGNGAIYDATILQLDTAQQLTDLLADPEIRPLIQPIPDAP
                     TLALVRATSRNRLRELLEARGMTLTLGGNRDSGFGGG"
     gene            complement(3219..5492)
                     /locus_tag="VFV93_14855"
     CDS             complement(3219..5492)
                     /locus_tag="VFV93_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166681.1"
                     /translation="MIRASPPASMQPFYDEDDVRRLCGLAVFAQADALQQAGHVLSAE
                     RASDTLSGDVRGVWRRVDRVTIQTRGNRLQPDCARHGAGFCQHAGALLLHWLRQPAAV
                     SLVLADPVDAGMNALMNPASLGLWPDDDALDDLLNGDPEFQDDEAAFNTPDAEIAGML
                     DQLTISVVREMARRRNIPVSGSRKAEVVEATAAGLADPANIAEALDRLSPSQLALLDA
                     IQLASLSGETTDASAFEIYRLLGGKGEPPLSSLTDLGLLFTFSPDFYRGRRLWIPRPV
                     VAALPPREGLATPLKGRPTGREPEPVSRALLPLLLMVAQDAQAGGISARDAATHLEYG
                     YIPDGFEVASADRERLDELLRTYNPNEWVRLIPTPLLPAADVGRLALQSGQPGGVVAF
                     VAQLMAVLEIAEQYPRLTVNDGMLRLLLEQPAFNQRQLLVAAWLRSPGWAEASMIFGD
                     DGPIRFGWNPRHAAPYVSSAIAEGVGLVARLAGRVPPGEWHDTSSLIEMAVQLTPSAA
                     PMLAQFRKSSQQVDFTWQGMPRPGQKLSLNSQDGLRLFLRAIADAVLGGPMFWLGLVD
                     VRLDDRNVTAFRTRQAAGLLTGKTFELDDLPESGPVRVSDDLTIVVPVGSASISALGF
                     ILSACQLGQATAEGMHYLLNANGAQALFEHGLSAADLASQLSAQTGQPLPPAAQETLD
                     RWWAGFGRVRLYDEVTLIELGDDLLLRELQAATSLGDVTIHQFSPRLIAVEDDAVDLL
                     VTELGARGYAPRIVEGG"
     gene            complement(5489..>7143)
                     /locus_tag="VFV93_14860"
     CDS             complement(5489..>7143)
                     /locus_tag="VFV93_14860"
                     /inference="COORDINATES: protein motif:HMM:NF045503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HEX5166682.1"
                     /translation="EEARDALARFAELEKSPEHIHTYRITPLSLWNAASSGYTAEYIV
                     TALERLAKYDLPGNVRTDVIDYVSRYGRVKLVREGERLLLVSEDPALLVEIEHHRHIK
                     PYLLGRDDSGRIIVDGARRGHVKQALVQFGYPAEDLAGYVDGAPLEIVLREESAASHA
                     PFGLRDYQREAVEIFHAGGSARGGSGVIVMPCGAGKTVVGLGVLAELQSQTLILCSSI
                     VAARQWIHEILDKTNIPADQIGEYSGEVKQIRPITVTTYQVLTWRANRSADFPHFKLF
                     AERDWGLIVYDEVHLLPAPVFRITAEIQARRRLGLTATLVREDRRETDVFSLIGPKRY
                     DVPWKDLEAQGWIATAECHEVRIRMPDELRFDYALAEEREKYRISAENPAKLPVLDEL
                     VRKFEGEQTLIIGQYISQVEEVAARYDAPLITGKTATREREKLYDAFRHGEQKLLVVS
                     KVANFSIDLPDASVAIQISGTFGSRQEEAQRLGRILRPKDDENLAHFYTLVTRDTRDQ
                     EFAANRQKFLTEQGYRYEILYEDEVLPTPPPVNVMAGEAADA"
BASE COUNT         1164 a         2432 c         2376 g         1171 t
ORIGIN      
        1 gtcggtcctc ttcttcgcag gcatcgcccc gaaattcgcc tcctcgcaca tccaactggc
       61 gttgattgga atggcgatcc tcgcattcgc agtcggcatc atccagatcg gcgggctccc
      121 gtcccagtga gacagcaccg ccattgtcat cctgcgtcgt gcgccgaagg atctctcctg
      181 cgctggtcga tcagagatcc ttcagcgcac gcctcaggat gacaaggcaa tgtttccggt
      241 ccacgctgca tgagcaaagg ttgacactcg atggcaatag actggctcca tgatgcggcc
      301 gaggcgccgg cgcaggcgtt gcgactgcgc cggccgatcc tgattgacgt ctacaaggtt
      361 ccctgacgcg gatgcgatcg gctggatgcc gatacgtacc ccgatccgga ggtcgccgag
      421 gcgatcggcg ggcgcttcgt gccgctcaag ctacacctgt tcgaggcgcc gcgtgaggtc
      481 atccggcccc tgaacgtgat ctggacgccg acactgctgg tcgccgaccg gcggctggcg
      541 gtccactacc gctcgctgaa cttcctgccg ccgcgagagt tcctgacgat gctcgatatc
      601 ggagaggccg agcattggct gcgctggagc aagagcgagc aggccatcgc actcctcgaa
      661 cgggccgagc agcgtgaccc aaccggcccg ctggccgccg aggcgatcta ccggcgcggc
      721 atcgccacct acctgctaac ccactccaac ccggagatgt acaccgtctg gcagacgctg
      781 ctggatcgat tcccggagag tgaatgggcg aagcgggtgc cgtaatgggg cgggattcgg
      841 gattcggagc cgaagagccg agcgcgtcct gaggaacgtc gaacgctacc cacccccgaa
      901 tcccgaatcc cgattcccgc ccaacgtcaa cgtcatcccc cgcgcttcca gcagctctcg
      961 caggcgattg cgtgaggtgg cccggacgag cgcgagggtc ggggcgtcgg ggatcggctg
     1021 gatcagcggg cgaatctcgg ggtcggccag caggtcggtg agttgctggg cggtgtcgag
     1081 ctggaggatt gtcgcgtcgt agatcgcgcc gttgccccag tccctggccc agcggcgcac
     1141 gagcgcggcc gggtcgccgg gcagcgtgcc gccggtgagc cgttcgaggg tggcgacaac
     1201 cgcatcggcg tcgaggccgt ttcgcgcgct gcggcgcagg ctgtccggtg tgatccggag
     1261 ctggccgttc tcggcatcga cgaatggctg tagctcgtgc agggtgtaga tggcaggctg
     1321 cggctggcgg aacgcgagcg tgccgtccgg ctcgacatcc agttgcgggc caacctgaaa
     1381 gcccggcccc tcgctgaggg cgaccaattg cccgttcgcg gtcaggctgc ggaagaccgt
     1441 ctgaagctgg tcagctggga cgagcgcgag cgtcgccgcc atccgccggc ccagcagcgg
     1501 gccgatcgac tcgtcggcgt agagcgcgtc gagcgtcgcc tcgtcgaccg tctggaccag
     1561 cggcgtccgt ttgcggagcg tgacccgttc gtgctgggcg ccccactcct ccagcgtccg
     1621 gcgcacgttc tggggtagct catgggtgct gaccgattcg aggaagcgga tcacctcgtc
     1681 ggtgtcgagg ccgccgcgct gggcgcggta catcgacgcc tgcgtcagct cgtactccat
     1741 cgcctggccg gtgcggatac gttcggcgat ccggtcgagc atgaacagca catcctcgcc
     1801 ggtcggctgg aaggcgaaga cctggaagtt gggctggacg atcacctggg cggccgtttc
     1861 gtcctccacc agcggctcac cggccagcag gcgccggcca tcgtcggtga tgcggaagac
     1921 ggttgtcgcg tcgcccttgc cgccgaggtc gacaacacca agtgcgtaga gcgcctcagt
     1981 cacgaccacc ctgatgaagc tggtctcggc gatgtcccag ccggagcgct cgttctcgat
     2041 gtcgagcgtg acgcccagcc cgttgacatt cgccacgtac gggttgtagt ggccgaagcc
     2101 gtagtagcgg tagttgacgg cgtagtagga gttcggcgat ggcgtccgcg agaacaggaa
     2161 ctcgtaggcg cgcagccgca ggcgggtgag gagatgctcg atcggtatcc actcgccggc
     2221 cggcaactcc gacagctcgg ccagcacccg ctggcgggcc ggcgggacgt tcgccggtgc
     2281 gtgatagatg ttgagtccct tgccacgcac gccgagatcg ggaacccggt gcagctccga
     2341 ccagcgggcc gtcgtccggt aggcgtcgaa cagtcgctgg cggcgctcgg ccgagggcag
     2401 cgccaggaac tgctcgccgc gcgggtcgag cagcagctca ccgacgctgc cggccagcaa
     2461 ccccagctcc tcggccagac cacgcagcag gctgaagccg gccagctgct cctcggtctt
     2521 gacggccgcg acctccttct cccgccccaa cacctggccg atcttcgtca gcgagcgctt
     2581 catgaccagg ccacggttgg tcagcgcgat cttctcgcga tcggccaggc tgaagagcag
     2641 gtagagctcg cgcaacagct ggccagcgtc actggtctgg accgtctctg gttccgcaac
     2701 cgtctcgact tccagctcga cctccggcag ctgctccaga atcgggtcgg ggatgatcag
     2761 tcgcttgccc ggctcggtga tcgagacgat gtagccgctc gaacgaactg gatcgagcgt
     2821 gaagagcagc ccgagtgcgc cgagccgcgc gaccaggtcc tcgaacttgc gcgagttgcg
     2881 gttgcggtac gagcccttct cgcgctggta gtacgagtag ccgtaatggg tcgtcttcgt
     2941 cggcttgtcg gtgatgccct cgccttcgag ctggttctgg agcagcgtcg tcggcgcctc
     3001 accaccggcc agaatgacgt ggtcgagcag cgcccgctcg gcgtcgctca ggtcgcccag
     3061 catccgcttg atccggccgg cgtcgaacaa cagcgccgcc agtgtcggga cgacgatgta
     3121 cttcccgccg ctaccgacct tgatcccctg tgccttcgcc atctgctgca aggaactgag
     3181 gtcgtagccc tgcaacagct ccccggcgga gatgtccatc agccaccctc gacaatccgt
     3241 ggcgcgtagc cgcgcgcgcc cagctccgtc acgagcagat cgacggcgtc atcctcgacc
     3301 gcgatcagtc gcgggctgaa ctggtggatg gtgacatcgc ccagcgatgt cgccgcctgt
     3361 agctcgcgca gcagcagatc atcgcccagc tcgatcaacg tcacctcgtc gtagagccgc
     3421 accctgccga agccggccca ccagcggtcg agtgtctcct gggcggccgg cgggagcggc
     3481 tggccggttt gcgcggagag ctggctggcg agatcggcgg cgctcagacc gtgctcgaac
     3541 agtgcctggg cgccattcgc gttgagcagg taatgcatgc cctctgccgt cgcctggccc
     3601 aactggcagg cactcaggat gaatcccagc gcgctgatgc tggccgagcc aaccggcaca
     3661 acgatcgtga gatcgtcgct gacgcggacc ggccccgact cggggagatc gtccagctcg
     3721 aacgtcttac cggtcagcag cccggctgcc tggcgggtgc ggaaggccgt cacgttgcga
     3781 tcgtcgagcc ggacatcgac cagcccgagc cagaacatcg gcccacccag gacggcatcg
     3841 gcgatcgccc gcaggaagag ccgcaatcca tcctgcgagt tgagcgagag cttctgaccg
     3901 ggccgtggca tgccctgcca ggtgaaatca acctgttgcg acgacttccg gaattgcgcc
     3961 agcatcggcg cggccgaggg cgtcagctgg actgccatct caatcaatga gctggtgtcg
     4021 tgccattcac ccggcggcac ccgaccagcc agccgggcaa ccaggccgac cccttcggca
     4081 atcgccgacg acacgtacgg cgcggcgtgg cgcgggttcc agccaaagcg gatcggccca
     4141 tcgtcgccga agatcatgct cgcctcggcc caaccgggcg accgcagcca ggcagcgacc
     4201 agcaactggc gctggttgaa tgccggctgc tccagcagga gacgcaacat gccgtcattg
     4261 accgtcagcc ggggatactg ctcggcgatc tccaggacgg ccatcagctg cgccacaaac
     4321 gccacaacgc cgccgggttg gccggattgg agcgccagcc gccccacatc ggcagcaggg
     4381 agcagcgggg tcggtatcag ccggacccat tcgttggggt tataggtccg cagcaattcg
     4441 tccagccgct cccggtcggc ggaagcaacc tcgaatccat ccggaatgta cccgtactca
     4501 agatgggtcg cagcgtcgcg cgctgaaatc cctccagcct gcgcatcctg ggcgaccatc
     4561 agcagcagcg gtagcagtgc ccggctgacc ggctccggct cgcgaccggt ggggcggccc
     4621 ttcagaggag tcgccagccc ctcgcgcggg gggagcgcgg caacaaccgg acggggaatc
     4681 cagaggcgcc gcccccggta gaagtccggc gagaaggtaa acagcagtcc gaggtcggtc
     4741 agcgagctca gcggcggttc gccctttccg ccaagcagtc ggtagatctc gaaggcactc
     4801 gcgtccgtcg tctcgccgga caacgatgcc agctggatcg cgtccagcag cgcaagctgc
     4861 gacgggctca accggtcaag cgcctccgcg atgttggccg gatcggccag cccggcggca
     4921 gtggcttcaa ccacctccgc cttgcgactg ccgctgaccg ggatattccg ccgccgcgcc
     4981 atctcgcgga cgacggagat cgtcagttga tccagcatgc cggcgatttc cgcgtcgggt
     5041 gtgttgaagg cagcctcatc gtcctgaaac tcagggtcgc cattcagcag gtcgtccagg
     5101 gcatcatcat ccggccagag accgaggctg gccgggttca tcaaggcgtt catcccggca
     5161 tcgactggat cggcgagcac cagcgatacc gccgccggct gccgcagcca gtgcagcaac
     5221 agcgcgcccg cgtgctggca gaagccggcg ccatgccggg cacagtcggg ctggagccgg
     5281 ttgccgcgcg tctggatcgt gacgcggtcg acccgccgcc agacgccgcg cacatcgccg
     5341 ctgagcgtgt ccgacgcccg ttcggcgctg aggacgtgcc cggcctgttg cagcgcatcg
     5401 gcctgggcga agacggccag cccgcacagc cggcggacat cgtcctcgtc gtagaacggc
     5461 tgcatcgagg ccgggggcga tgcccggatc atgcgtcggc tgcctcgccg gccatcacat
     5521 tgacaggtgg cggcgttggg agcacctcat cctcgtagag gatctcgtag cggtagccct
     5581 gctccgtcag gaacttctgg cgattggcgg cgaactcctg gtcgcgggtg tcgcgggtga
     5641 cgagcgtgta gaagtgggcc aggttctcgt cgtccttcgg ccgcagaatg cgtcccagcc
     5701 gctgggcctc ctcctgtcgc gagccgaagg taccggagat ctggatcgcg acgctggcgt
     5761 ctggcaggtc gatcgagaag ttggccacct tcgagacgac cagcagcttc tgctcgccgt
     5821 ggcggaaggc gtcgtagagc ttctcgcgct cgcgggtggc cgtcttgccg gtgatcagcg
     5881 gcgcgtcgta gcgggcggcg acctcctcga cctggctgat gtactggccg atgatcagcg
     5941 tctgctcgcc ctcgaacttg cgcaccagct cgtccagcac cggtagcttg gccgggttct
     6001 cggctgagat gcggtacttc tcgcgctcct cggccagcgc gtagtcgaag cgcagctcgt
     6061 ccggcatccg gatgcgcacc tcgtggcact cggccgtcgc gatccagccc tgcgcttcga
     6121 ggtccttcca gggcacgtcg tagcgcttcg gtccgatcag cgagaagaca tccgtctcgc
     6181 gccggtcctc gcgcacgagc gtcgccgtca gtccgagccg ccgccgcgcc tgaatctcgg
     6241 ccgtgatgcg gaacacgggc gccggcagca ggtggacctc gtcgtagacg atcagccccc
     6301 aatcccgctc ggcgaacagc ttgaagtgcg ggaaatccgc cgaccggttc gcccgccacg
     6361 tcagcacctg ataggtcgtg acggtgatcg gccggatctg cttgacctcg ccgctgtact
     6421 cgccgatctg gtcggccggg atgttggtct tgtccagaat ctcatggatc cactgccggg
     6481 cagcgacgat cgacgagcag aggatcagcg tctgcgattg cagttcggcc agcacaccca
     6541 ggcccacaac ggtcttgccg gccccgcagg gcatcacgat cacgccgctg ccgccccgcg
     6601 ccgacccacc ggcgtggaag atctcgactg cctcgcgctg gtagtcgcgc agcccgaacg
     6661 gcgcgtgact ggcagccgac tcctcgcgca ggacgatctc cagcggtgcg ccatcgacgt
     6721 aaccggccag gtcctcggcc ggatagccga actggaccag cgcctgcttg acatgcccgc
     6781 gccgcgcgcc atcgacgatg atccgcccgc tgtcgtcgcg ccccagcagg tacggcttga
     6841 tgtgccggtg atgctcgatc tcgaccagca gggccgggtc ctcggagacg agcaacagcc
     6901 gctcgccctc gcgaaccagc ttgacgcgcc cataacggga gacgtagtcg atgacatcgg
     6961 tgcggacgtt gcccggcagg tcgtacttcg ccagccgttc gagagccgtg acgatgtact
     7021 cggccgtgta gcccgacgag gccgcgttcc agagcgagag cggcgtgatc cggtaggtgt
     7081 gaatgtgctc cggactcttc tccaactcgg caaagcgcgc caacgcgtcc cgtgcctcct
     7141 cgt
//
LOCUS       DASUGJ010000652         5356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_105966, whole genome shotgun sequence.
ACCESSION   DASUGJ010000652 DASUGJ010000000
VERSION     DASUGJ010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105966"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VFV93_14865"
     CDS             complement(<1..1319)
                     /locus_tag="VFV93_14865"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX5166683.1"
                     /translation="MEDATPIPETAPATNSAGLTHKQVLLVFSGLMLGMLLAALDQTI
                     VATALPTIVGELGGLEHLSWVITAYLLASTTSTPLYGKLSDIYGRKPLFQLAIVIFLL
                     GSVLSGVSQSMLQLILFRALQGLGAGGLMALAQAIIGDILSPRERGRYQGYIGSVFAF
                     SSVVGPLLGGLFVDHLSWRWVFYVNVPIGAVALVVTSIVLKLPVRRRQHSIDYIGSAL
                     LVLGIGALLLMTSWGGSEYAWDSPQILALGIGGALAILAFLWQETRAAEPVLPLRLFR
                     ERTFAVGSGLGMVVGLAMFGSIAFLPVYFQVVRNASATESGLRMVPMMFGIVSMSIIS
                     GRLITKTGKYRIFPIVGTAVMAVGMLLITRMGTDAGQLQTAVSLLVLGVGVGMVMQVI
                     VLAVQNAAPPGDLGIATAGVNLFRSMGSAFGVAIFGAILANRLDVELA"
     gene            complement(1320..1814)
                     /locus_tag="VFV93_14870"
     CDS             complement(1320..1814)
                     /locus_tag="VFV93_14870"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5166684.1"
                     /translation="MSALSVSESIANALSALRRGPRVQRLHRLVMERAGVDLDRPALT
                     ALTVLATSGPLRVSELAEVCAVDISTMSRLAGRMTCSGFVVQSTDPNDGRVVMLELTD
                     DGREATQRMVDAKTALIGEVIADWSADDRAAFAALLDRFVIDLERTNRLAIERERDTT
                     GVSR"
     gene            complement(1878..3185)
                     /locus_tag="VFV93_14875"
     CDS             complement(1878..3185)
                     /locus_tag="VFV93_14875"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166685.1"
                     /translation="MTATLEERRRHSPRAALRQTFRALRNWNYRMFWLGQVVSTIGTW
                     MQRIGLAWYVLDKTDSAFALGLVTMFQTLPVLLLALIGGVIADRFPKRDLLFWTQGTL
                     MLQSAGLAVLTAIGTSNLIPIYVLATIQGIAKAIDNPTRQAFVKELVGPNDVPNAVAL
                     NSIVFNSARLIGPALGGGVIALVGVPGCFTIDAISFLGVLIGLFLMKPEKFYDIAGPQ
                     RGRMLGQISEGIRYAVRTPEIAVVLLLMLVVGTFGYNFTVLLPLIAEYVLDTGPVGFG
                     VLTSTMAVGSLTAAVGIAYTGRVSQKTLLVGASGFTALLFCLSLTTTWATTILILAPL
                     GLFSIVFTATANSRLQIITPPELRGRIMSLYTLMFLGSTPIGSLIIGSLAEHQGVRMA
                     TAEVAGLCAVGIGLTFGYLYLHRPEQDESRALEQQVVREHTVR"
     gene            complement(3182..3661)
                     /locus_tag="VFV93_14880"
     CDS             complement(3182..3661)
                     /locus_tag="VFV93_14880"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5166686.1"
                     /translation="MPSDQLVEDLLFLWRLLRQSTHPAYRGEITLEQYWLLRILRRTA
                     EDGLSIGELAARTGVGQSSITTACKRLEKAGLVSRRRDARDERVVRVSLTAEGVEQVE
                     QWRHAKRAALTQLLEPLTAGERRTLEQLMRRVVELGSDDAVIGWSFQPVETQETGDR"
     gene            3970..5172
                     /locus_tag="VFV93_14885"
     CDS             3970..5172
                     /locus_tag="VFV93_14885"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5166687.1"
                     /translation="MVHKNVRHPRRRWKLASATLVLLLVSLSLASCSSPGDPMSTATT
                     VPTTSTLVPTVDSTSTSTLPRVATGAPSPTPSPTDPPTATEPPPLNPEEFAAEVTGLV
                     DSLDGLVEVVVTRPDGTTLADINSYEPMEAASLYKLGIMVEIYVEREMGELALDEELI
                     LEPEYFTPEDSAFTDDDIGISVSIETLLQSMITLSSNVAASALLARVGNENVNLTMAS
                     LGLSSTEIRWSPGVDPYVPDDDATVPEDDSTEDPESEPTVEDVSESDNDGALSARREL
                     LGLPGRGSLDDARADAALNVTTAADIASLYVMLVNGQIISEEASQEMLTLLEDQQIND
                     RLPAYLPEGTIVAHKTGNLDGLVHDAGVIFTPSGPVVVVIMTEDVEEWQAIDLIATVA
                     RLAYDSVP"
BASE COUNT         1043 a         1879 c         1552 g          882 t
ORIGIN      
        1 gccagctcga catccagccg gttcgcgagg atcgcgccga agatcgccac tccaaatgcg
       61 cttcccattg agcggaacag gttcacgccg gctgtcgcga tgcccagatc gcccggaggc
      121 gccgcgttct gaacggccag gacgatgacc tgcatcacca tcccgacgcc gacacccagc
      181 accagcagcg agacggccgt ctgcaactgg cccgcgtcgg ttcccatccg cgtgatgagc
      241 agcatcccaa ccgccatcac ggctgtcccg acgatcggga agatacggta cttgcctgtc
      301 ttggtgatca gccgtccaga gatgatcgac atcgaaacga tcccgaacat catcggcacc
      361 atccgcaggc cggattccgt cgccgaggcg ttgcgcacga cctggaagta gaccggcagg
      421 aacgcgatcg agccaaacat cgccaggccg acgaccatcc ccaacccgga gccgaccgcg
      481 aacgtccgct cacggaagag ccgtagcggc aggaccggct cggccgcgcg cgtttcctgc
      541 cagaggaagg ccaggatcgc cagcgcgccg ccaattccca gcgcgagaat ctgcggcgaa
      601 tcccaggcgt actcgctgcc accccagctg gtcatcagca acaaggcacc gatacccagc
      661 accagcaagg ctgaaccgat gtagtcaatc gagtgctgcc gccgccgcac cggcagcttc
      721 agcacgatcg atgtcacgac cagcgcgacc gccccgatcg gtacgttcac gtagaacacc
      781 cagcgccagc tgagatggtc gacgaacagg ccgcccagca gtgggccaac gacgctcgaa
      841 aaggcgaaga ccgagccgat gtatccctgg tagcgcccac gctcgcgtgg cgagaggatg
      901 tcgccgatga tcgcctgcgc cagggccatc aggccgccgg cgccgaggcc ctgcaacgcc
      961 cggaacagaa tcagttgcaa catcgactgc gacacgcccg agaggaccga gccgagcagg
     1021 aagatcacga ttgcaagctg aaagagcggc ttgcggccgt agatgtcgct cagcttgccg
     1081 tacagcggcg tcgaggtcgt cgacgccaac aggtaggccg taatcaccca cgagagatgt
     1141 tccagcccgc ccagctcgcc aacgatcgtt ggcagcgccg tcgcgacgat cgtctgatcg
     1201 agcgcggcca gcagcatgcc gagcatcagc ccgctgaaga ccagcaggac ctgcttgtgg
     1261 gtcagcccgg cgctatttgt cgctggtgcc gtttccggta ttggtgtggc gtcttccatt
     1321 cagcggctca ctcctgtggt atcgcgctcc cgctcgattg ccaatcgatt ggtcctctcc
     1381 aggtcgatca cgaaccggtc gagcagcgcc gcgaaggccg cccggtcatc cgcgctccag
     1441 tcagcgatga cctcgccgat cagcgccgtc ttggcgtcaa ccatccgctg cgtcgcctcc
     1501 cggccatcat cggtcagttc gagcatcacg acgcgcccgt cgttcggatc ggtcgactgc
     1561 acgacgaagc cgctgcacgt cattcgaccg gccaggcggc tcatcgtcga gatatcgacg
     1621 gcacagacct cggccagctc gctgacccgc aacggcccgg atgtcgccag caccgtcagc
     1681 gcggtcaacg ccggccggtc caggtcaacg ccggcgcgtt ccatcaccag ccggtgcagg
     1741 cgctggacgc gcggcccgcg ccgcaacgct gacaacgcgt tggcgatcga ttcgctgaca
     1801 gataatgcac tcatgctcat atgcgggatt ctaactcagt tgcttgcagc acgcaactac
     1861 ctgtacgacc agacagtcta ccgtaccgtg tgttcgcgga cgacctgttg ctccagcgcc
     1921 cggctttcgt cctgctcggg gcgatgcagg tacagatagc cgaatgtcaa cccaattccc
     1981 acggcgcaca gcccggcaac ctccgccgtc gccatccgca caccctgatg ctcggccagt
     2041 gagccgatga tcaggctacc gattggagtc gatccaagga acatcagcgt gtacaggctc
     2101 atgatccggc cgcgtagctc cggcggcgta atgatctgga gcctgctgtt ggcggttgcc
     2161 gtgaagacga tgctgaacaa ccccagcggc gccagaatca gaatcgtcgt cgcccaggtc
     2221 gtcgtcagcg acaggcagaa cagcagcgcc gtgaacccgc tggctccaac cagcagcgtc
     2281 ttctgtgaca cccggccggt gtaggcgatt cccaccgccg ctgttaacga gccaactgcc
     2341 atcgtcgatg tcagcacgcc aaatccgacc ggaccagtat ccagcacgta ttccgcgatc
     2401 agcgggagca gaaccgtgaa gttgtagcca aacgtgccga caaccagcat cagcagcagg
     2461 acgacagcga tctccggcgt ccgaaccgcg taccggatac cctcggagat ctgcccgagc
     2521 attcgccctc gctgcgggcc ggcgatgtcg tagaacttct ccggcttcat caggaacaac
     2581 ccgatcagca cgcccaggaa gctgatcgcg tcaatcgtga agcagccggg cacgccgacc
     2641 agggcgatca cgccgccacc gagcgccggc ccgatcagcc gcgccgagtt gaacacgatc
     2701 gagttcaacg ccactgcgtt cggcacgtcg ttcggcccga ccagctcctt gacgaacgcc
     2761 tgccgtgtcg gattatcgat cgccttcgca atgccctgaa tcgtcgccag cacgtagatc
     2821 gggatcaggt tcgacgtccc aatcgccgtc aggaccgcca gcccggccga ctggagcatc
     2881 aatgtgccct gtgtccagaa cagcaggtcc cgcttcggga accggtcggc gatgacgccg
     2941 ccgatcagtg ccagcagcag gaccggcagc gtctggaaca tcgtgacgag cccgagcgcg
     3001 aaggcggagt cggtcttgtc gaggacgtac caggccagcc cgatccgctg catccaggtg
     3061 ccgatcgtcg acacgacctg gccgagccag aacatccggt agttccagtt acgcaatgcc
     3121 cgaaacgttt gtcgcagtgc ggcgcgtggc gaatgcctgc gacgttcttc gagcgttgct
     3181 gtcatcggtc cccggtctcc tgtgtttcta ccggctggaa tgaccagccg atcactgcgt
     3241 catcgctgcc caactcgacc acccgccgca tcagctgttc gagcgtgcgt cgctcacccg
     3301 cagtcagcgg ctccagcagc tgtgtcaacg ccgcccgctt ggcgtgccgc cactgctcga
     3361 cctgctcgac gccctctgcc gtcagcgaga cgcgcacgac ccgctcgtcg cgcgcgtcac
     3421 gccggcgcga gaccagcccg gctttctcca gccgtttgca ggcggtcgtg atcgagctct
     3481 gcccgacgcc ggtcctggcc gccagctcgc cgatgctcag cccatcctca gccgtccggc
     3541 gcaggatccg cagcagccag tactgctcca gcgtgatctc accccgatag gcgggatgcg
     3601 tcgactggcg tagcaagcgc catagaaaca ggagatcttc gacaagctga tcggaaggca
     3661 cggactcaat tccatcactg atcgaatcta tcgcctgtca atgtaccacg tgaaccacga
     3721 gccgtcgagc cttcccacaa ctcggttcga gcgatggggt cggactactc gaacaccgcg
     3781 gaaggagggg ccgtgacgcg tttcgagtat tcgcatctcc catcccccat gccccatcag
     3841 tcatccccaa aaccgtgacc tccggaaccc aactcgcgcc gcccgcacgt gatatgctcc
     3901 cgcactgccg gtcgatgtcg ttcgaggcgc ctgccgtacg ttgacaggtc cagtccgcgt
     3961 ggagtaatcg tggtccacaa gaatgttcgt cacccgcgcc gacgctggaa gctcgcatcc
     4021 gccacactcg tcctgctgct cgtcagcctc tcactggcgt cctgtagctc tcccggcgac
     4081 ccgatgtcga ctgccacgac cgtgccgacg acgtccacac tggtcccgac ggttgattcg
     4141 acctcaacct ccacgctgcc acgagtcgcg accggtgctc cctcgccgac gccatctcca
     4201 accgatccgc cgaccgcgac cgagccgcca ccgctcaacc cggaggaatt cgccgccgag
     4261 gtcaccggcc tcgttgatag tctcgacggc ctcgttgagg tcgtcgttac ccgaccggac
     4321 ggcacgacgc tcgccgacat caacagttac gagccgatgg aggccgccag cctctacaag
     4381 ctcggaatca tggtcgagat ttacgtcgag cgtgagatgg gcgaactggc gctggacgag
     4441 gaacttatcc tcgaaccaga gtatttcacc ccggaggata gcgccttcac tgacgacgac
     4501 attggcatct ccgtctcgat cgagacgctg ctgcaatcga tgatcacgct ctccagcaac
     4561 gtcgctgcct cggccctgct ggcccgtgtc ggcaacgaga acgtcaacct gacgatggcc
     4621 tcgctcggtc tctcttcgac cgagatccgc tggtcgccgg gagttgatcc gtatgtccct
     4681 gacgatgacg cgaccgtgcc ggaagacgac agcaccgaag atccggaaag tgaacctacc
     4741 gtcgaggacg tctcggaaag cgacaacgat ggcgcgctct ctgcccgccg ggaactgctc
     4801 gggctgcccg gccgcgggtc actggacgat gcccgcgcgg acgccgccct caacgtcacg
     4861 accgccgctg acatcgcctc gctctacgtc atgctcgtca atggccagat catcagcgaa
     4921 gaagccagcc aggagatgct gacgctgctc gaagaccagc agatcaacga ccggctgccc
     4981 gcttacctgc cggaagggac gatcgtcgcc cacaagaccg gcaatctcga cggcctcgtc
     5041 cacgacgccg gcgtcatctt caccccgtcc ggcccggtcg tcgtcgtcat catgaccgaa
     5101 gatgtcgaag agtggcaggc gatcgacctg atcgccaccg tcgcccgcct cgcctacgac
     5161 agcgtcccct gatcgcatcc ggacaatcaa tatccctcaa tccggtatgg gcagcaaaaa
     5221 gggcgatatt gcgatatcgc ccataccggc ttgagggata ttgattgtcc ggatgcgatc
     5281 cggggacgct gtcgtaggcg aggcgggcga cggtggcgat caggtcgatc gcctgccact
     5341 cttcgacatc ttcggt
//
LOCUS       DASUGJ010000653         5653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1774730, whole genome shotgun sequence.
ACCESSION   DASUGJ010000653 DASUGJ010000000
VERSION     DASUGJ010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774730"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VFV93_14890"
     CDS             <1..1118
                     /locus_tag="VFV93_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5166688.1"
                     /translation="IGRRLARRAVALVPGTATQPATTDLVDLTRSAASTPSPAVAAAA
                     HAALDRGETHYTARPGIPQLCAALAARSAGEGFPADAESVVVTNGGSEALYIALQSVA
                     RPGSRVLLAGPVAPNIPKMIAFIGATSIQVDSTASSPTAATIAGYDADVLLLASPSPI
                     TGVAIPPDELAAIVRQAGERGMAVILDRSLAWCCYDPVLAHFPDPDLGATILTTGSFS
                     LAYEMAGWRAGYFTAPAQHAGAMLELKQAMSICTSAISQFAALAALEAADDWLNTKRS
                     AMQTRRDAVILRLEQAGLSTIVPDSWPFLLLDTRLAHPDDRQAADMLREQAGVIVEPA
                     SIYGPSLAGYARITLAAGETALNDGLDRLIRFHSACS"
     gene            1150..2283
                     /locus_tag="VFV93_14895"
     CDS             1150..2283
                     /locus_tag="VFV93_14895"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5166689.1"
                     /translation="MSVNIFDLMELAKRKPNAISLGLGDPDLPTPAHIVAAARQAIDE
                     GRSGPAPATGLLELREAIARKLARDNGIEVDPDSEVLVTTGGQEALFLLVQSLIEPGD
                     EVLLPDPRYTSYDNAIELAGGRMVLVPTDEAHAFELDPDEVERRITGKTKLLLLISPN
                     NPTAGINTPEVVRRLAEIAIEHDLIVIADELYEKFVYDGYQHLSIGSLPGMRERTITL
                     NGLSKTYAMTGWRIGYLAAPADLIRTATALKQMVNVQAPTISQWAAVAALDGPQACVA
                     EMRAIYDERRRLLMPALRGMGFSFGEPRGGLYIWLNIASTGMDSTALSYRFLQEADVL
                     ILPGTGFGDRWHDYMRMTILQPKEILADVVERMQTVLAQHAMG"
     gene            2300..2695
                     /locus_tag="VFV93_14900"
     CDS             2300..2695
                     /locus_tag="VFV93_14900"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX5166690.1"
                     /translation="MSATTLANQLEARIRQELPSLTIDESEELARVLDILVQIFRPKI
                     AYVFGSQARGDTTADSDVDLMLVVTSSDEPSYRRAQAAYRAINSHFLPIDILIWTREE
                     FDRRRPNSATLPGTIVREGKVLYATAIPG"
     gene            2673..3089
                     /locus_tag="VFV93_14905"
     CDS             2673..3089
                     /locus_tag="VFV93_14905"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX5166691.1"
                     /translation="MQPQYLADADDWLERSERDLETAKRNLQIHPLLGEVVAYHAQQS
                     AEKALKRFLTAYGLPFRKTHLLESLVKTCEMIDHQFQQFTASAQTLSPYAIEFRYPGG
                     RLEPSEAEARNAVQYATDILQFVRDTLANATPRSSN"
     gene            3259..3516
                     /locus_tag="VFV93_14910"
     CDS             3259..3516
                     /locus_tag="VFV93_14910"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5166692.1"
                     /translation="MSIIGWIVIGAVAGWLASIIMKRNDSMNWLENIIVGIVGALVGG
                     FLYGLLTDSDYTGGFNVGTLLIAVVGAVILLAIYGAVRREA"
     gene            complement(3616..4089)
                     /locus_tag="VFV93_14915"
     CDS             complement(3616..4089)
                     /locus_tag="VFV93_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5166693.1"
                     /translation="MDHARMHQQTGQIGLPDDVPSYPNGSGPLQRRISAAGQPVEPEL
                     RDTASLAMQYATIALLDGTVIGRLACDRLLGEIVVGRGSLAGVRVDDPFVHRVHSEIR
                     WDADLRSHVIAHAGGSNGTYVNLQRVDRPTRLIDGARIRIGKTELIYRRVYYPGD"
     gene            4280..5296
                     /locus_tag="VFV93_14920"
     CDS             4280..5296
                     /locus_tag="VFV93_14920"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEX5166694.1"
                     /translation="MEFQTGHRAIFRSAALLTGLLLAVTLLLPACGSSDDDNDSPLPT
                     ASATSAGAAPTEPASSPTGDEATSTATTAPATATPVAPSPTASTATQASPTAVANDQV
                     TILSIFFLRDEKIATVHRTVPKTQQVAAAAMAQLLAGPTAEDAAAGMTTAIPEGTEYI
                     GTTIAEGVATVDLSSEFESGGGSLSMSARLAQVVYTLTQFPTVDFVTFTLDGEPVTTF
                     GGEGIVLDRPVSRKDFEDLTPIIFVASPAVNDTVSSPLRVTGTANTFEAAFMLQILDA
                     NGATLVEQPMMATSGTGTRGTFDATIEFNAPPGSKITLRVFEHSAKDGSEVNVVEIPL
                     TVAE"
     gene            complement(5332..>5653)
                     /locus_tag="VFV93_14925"
     CDS             complement(5332..>5653)
                     /locus_tag="VFV93_14925"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166695.1"
                     /translation="DIVASPRHADLLLITGSVTRHLEQAALTAYEAMPEPRLVVAVGA
                     CACGGGVLQGSYCTAGGVPNVLPVDVFIPGCPPRPPALIHGLLVALERRVPKLQQTQL
                     TVSV"
BASE COUNT         1009 a         1918 c         1809 g          917 t
ORIGIN      
        1 tgatcggcag gcgactggcg cgacgggcag tcgcgctcgt gcccgggacg gcgacgcaac
       61 cagccacaac tgacctggtc gatctaacgc ggtcagcggc ctcaacacca tcgcccgccg
      121 ttgcggcggc cgctcatgcc gcgctcgacc gaggcgagac gcactacacg gcccgacccg
      181 gcatcccgca gctgtgcgcc gcgctggcgg cccgctcggc cggcgagggg ttcccggccg
      241 atgccgagtc ggtcgtcgtc accaacggcg gctcggaggc actctacatc gcgctccagt
      301 cagtcgctcg gccgggaagc cgggtgctgc tggccgggcc ggtcgcgccg aacatcccga
      361 agatgatcgc cttcatcggc gcgacctcga tccaggttga cagcaccgcc tccagcccga
      421 cggccgcgac gatcgccggc tacgacgccg acgtactgct gctggcgtcg ccctcgccga
      481 tcaccggtgt ggcgatcccg ccggacgagc tggcggcgat cgtccggcag gccggcgagc
      541 gaggaatggc ggtcatcctc gaccgctcgc tggcgtggtg ctgctacgat ccggtgctgg
      601 cgcattttcc tgatccggat ctcggggcga cgatcctgac gaccggctcc ttctcgctgg
      661 cctacgagat ggctggctgg cgggccggct acttcacggc cccggcccag cacgccggcg
      721 cgatgctgga gctgaagcag gccatgtcga tctgtaccag cgcgatctcc cagttcgccg
      781 cgctggccgc gctcgaagcg gccgacgatt ggctcaacac gaagcgctcg gcgatgcaga
      841 cgcgccgcga cgccgttatc ctgcggctgg agcaggccgg gctgtcaacg atcgtccccg
      901 atagctggcc cttcctactg ctggacacgc ggctggccca ccccgacgac cgccaggcgg
      961 ccgacatgct gcgcgagcag gccggcgtca ttgtcgagcc agcctcgatc tatggaccat
     1021 cgctcgccgg ctacgcgcgc atcacgctcg cggccggtga aacggcattg aacgacggcc
     1081 tcgaccggct gatccgcttc cacagcgcct gttcgtagcc gagacgttct cgcggggaga
     1141 gaggggccga tgtccgtcaa catcttcgac ctgatggagc tggcgaagcg caagccgaac
     1201 gcgatctcgc tcgggctggg cgacccggac ctgccgacgc cggcgcacat cgtcgcggcc
     1261 gcccgccagg ccatcgacga gggccgcagc ggcccggccc cggcgaccgg cctgctggag
     1321 ctgcgcgagg ccatcgcccg caagttggcg cgcgacaacg gcatcgaggt cgatccggac
     1381 agtgaggtac tggtgacgac cggcggccag gaggcgctct tcctgctggt gcaatcgctg
     1441 atcgagccgg gtgatgaggt gctactgccc gacccgcgct atacctccta cgacaacgcg
     1501 atcgagctgg cgggcggccg gatggtgctg gtcccaaccg acgaggcgca cgccttcgag
     1561 ctggaccctg acgaggtcga acgacggatc acgggcaaga ccaagctgct gctgctgatc
     1621 tcaccgaaca atccgacggc cggaatcaac acgccggagg tcgtccgccg gctggccgag
     1681 atcgcgatcg agcatgatct gatcgtgatt gccgacgagc tgtacgagaa gttcgtctat
     1741 gacggttacc agcacctcag catcggctca ctgccgggca tgcgcgagcg gacgatcacc
     1801 ctcaacggac tctcgaagac ctacgcgatg accggctggc gaatcggcta cctggccgcc
     1861 ccggccgacc tgatccggac ggcaaccgcc ctgaagcaga tggtcaacgt ccaggcgcca
     1921 acgatttcgc aatgggccgc cgtcgccgcc ctcgacggcc cacaggcctg cgtcgccgag
     1981 atgcgcgcga tctacgacga gcgccgccgg ctgctgatgc cggccctgcg ggggatgggc
     2041 ttcagcttcg gtgagccacg cggcgggctc tacatctggc tcaatatcgc gtcaaccggc
     2101 atggactcaa cggccctctc ctaccgcttc ctccaggagg ccgatgtcct gatcctgccc
     2161 ggcaccggct tcggcgaccg ctggcacgac tacatgcgga tgacgatcct ccagccgaag
     2221 gagatcctgg ccgacgtcgt cgaacggatg cagaccgtcc tcgcccagca tgcgatgggc
     2281 taactggcac gatagcgaca tgagtgcaac gacgctcgcc aaccagcttg aagcgcgcat
     2341 ccggcaggag ttgccgagcc tgactatcga tgagtcggag gaactggccc gcgtacttga
     2401 catccttgtc caaatctttc ggccgaagat cgcctacgta ttcggctctc aagcacgcgg
     2461 cgacacgact gccgacagcg atgtcgatct gatgctcgtc gtgacatcat ccgatgagcc
     2521 gagctacagg cgtgcacagg ccgcgtaccg cgccatcaac tcgcacttcc tgccaatcga
     2581 tatcctcata tggacacgcg aagaattcga ccggcgccgg ccaaactcag ccacactgcc
     2641 aggaacgatt gttcgcgaag gtaaggtact gtatgcaacc gcaatacctg gctgacgccg
     2701 acgattggct tgagcggtca gagcgcgatc ttgaaactgc gaagcgtaat ttgcagatac
     2761 acccgctcct cggcgaagtt gttgcctacc acgctcagca gtccgcagag aaggcattga
     2821 agcgctttct gacggcttat ggcctcccgt ttcggaaaac gcatctcttg gagtccctcg
     2881 tcaagacctg cgaaatgatt gatcatcaat tccagcagtt cactgcttcg gcgcaaactc
     2941 tgagcccgta tgcaatcgaa ttccgctacc ctggtgggcg gcttgagccg tcggaagccg
     3001 aggcacgcaa cgccgtgcag tacgcgaccg acatcctcca gttcgtgcga gacacgctcg
     3061 ccaacgccac tccccgatca tcgaactgac agcctcttca gatcatcccg ttggccgcag
     3121 gccgtggcac accgtttgca tttagagcgt cgtctatccg gcgtctgatt gccggaaacg
     3181 tgaggtgcga ggcagggatc tcgcgcacac ggatgtggag tattcgtgca ggctagctgc
     3241 acagaaggga tgctgatcgt gagcatcatt ggttggatcg tcatcggcgc tgtcgccggt
     3301 tggctggcca gtatcatcat gaagcgcaat gattcgatga actggcttga gaacatcatc
     3361 gtcggcattg tcggcgcact ggtcggcggg ttcctgtatg ggctgctgac cgattcggac
     3421 tacactggcg gcttcaacgt tggcacgcta ctgatcgccg tcgttggcgc ggtcatcctg
     3481 ctggccatct acggcgccgt ccgtcgcgaa gcctgacgca cgccccaagc gtgcggacag
     3541 ggtcatcaat acgaaacgcg agcaccccgg tccaggacgg ccggggtgtt cccatgtctg
     3601 agacagagca gtggatcagt cgccagggta gtagacgcgc cggtagatca gctcggtctt
     3661 gccgatccgg atgcgggcgc cgtcgatcag tcgggttggc cggtcaaccc gctgcaggtt
     3721 gacgtaggtg ccgttgctgc cgccggcgtg ggcgatgacg tgcgagcgca ggtctgcatc
     3781 ccagcgaatc tccgagtgga cgcgatggac gaagggatcg tcgacgcgga cgccggccag
     3841 gctgccgcgg ccaacgacga tctcccccag caaccggtca cacgccagcc gcccgatgac
     3901 cgtgccatcc agcagcgcga tcgtcgcgta ctgcatcgcc aggctggcgg tgtcgcggag
     3961 ttcgggttcc accggctggc cggccgcgct gatccgacgc tggagcgggc cggacccgtt
     4021 cgggtatgaa gggacgtcgt cgggcaggcc gatctgtccg gtctgctggt gcatgcgtgc
     4081 gtggtccatc cgggaatcgt ccacctcttc tgccggcgac gacctccgga gcggtgcgtc
     4141 gatgacactc tggcaactcg cttgcgacga cgggcaccga cagccagaca gccgtcagca
     4201 actgcccgtg accttcgatt gccatcatcc ttgttggaac tatagttgat cgctcatcag
     4261 tcaggaagta ggacaagagg tggaattcca gaccgggcat cgcgccatat tccggtcggc
     4321 tgcactgctg actggcttgc ttctggccgt aacgctgctg ctgccagcct gtggcagctc
     4381 agacgatgac aacgattctc cgctcccgac cgcgtccgca acctcggccg gtgcagcgcc
     4441 gaccgaacca gccagcagcc caacgggcga tgaagcgaca tcaaccgcga cgaccgcacc
     4501 ggcgaccgca acgccggtcg cacccagccc aacggcatcc accgccaccc aggccagccc
     4561 gacagccgtc gccaacgacc aggtcacgat tctctccatc ttcttcctgc gtgacgagaa
     4621 gatcgcgact gtccaccgaa cggtgccgaa gacccagcag gtcgccgcag ccgcgatggc
     4681 gcaattgctg gccggaccga ccgccgagga tgcggcggcc gggatgacga cggcgatccc
     4741 ggaaggcacc gagtacatcg ggacgacgat cgccgagggc gtcgcgacgg ttgacctgtc
     4801 gagcgagttc gagtcgggcg gcggcagcct gtcgatgtcg gcccggctgg cgcaggtcgt
     4861 ctacacgctg acgcagttcc caaccgtcga cttcgtcacg ttcacgctgg atggcgagcc
     4921 ggtgacgacc ttcggcggcg aggggatcgt cctcgaccgg ccggttagcc gcaaggattt
     4981 cgaggacctg acgccgatca tcttcgtcgc gtcaccagct gtcaacgaca ccgtcagcag
     5041 cccgctgcgc gtcaccggca ccgccaacac gttcgaagcc gccttcatgc ttcagatcct
     5101 cgacgcgaac ggcgcaaccc tggtcgagca gccgatgatg gcgacgtccg gcaccggcac
     5161 acgcggcacc ttcgacgcca cgatcgagtt caacgcccca ccgggcagca agatcacgct
     5221 gcgtgtcttc gagcactccg cgaaggatgg ctcggaggtc aacgtcgtcg agattccgct
     5281 gaccgtcgca gagtagcgct cgcgggaccg ggccacagcc ggcggcgtcg atcagacgga
     5341 gacggtcaac tgtgtctgct ggagcttcgg cacccggcgc tcgagtgcga ccagcagacc
     5401 atgaatcagc gccggcggac gcggcggaca gccggggatg aagacatcga ccggcaggac
     5461 gttcggcacg ccgccggctg tgcagtagct accctgcagg acgccgccac cacaggcgca
     5521 ggcgccgacc gcgacgacca gccgtggctc cggcatcgcc tcgtaggccg tcagcgccgc
     5581 ctgctccagg tggcgcgtca cgctgccggt gatcagcagc aggtcggcgt ggcgcggcga
     5641 ggcgacgata tcg
//
LOCUS       DASUGJ010000654         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_662120, whole genome shotgun sequence.
ACCESSION   DASUGJ010000654 DASUGJ010000000
VERSION     DASUGJ010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662120"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFV93_14930"
     CDS             <1..760
                     /locus_tag="VFV93_14930"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5166696.1"
                     /translation="PDLPGQPRGARYMNSIGPLARDIDDLALALSIIEGPDDFDLDVP
                     PTPADGLPSLPDMDLDGVRFAVATNFPGLPVAAGIRAAIERVAGLIEREGCHVEERLP
                     EIDFDEQLETRATLRRYVRLFTEPPAEPLPAAAYFHALDRRDHFIRAWQAFLNDTILI
                     CPVMMTTAFPHCPRDTPVTVDGVEHPYSQIADYCRPFNLTGQPVVTIPAGLDDAGLPI
                     GIQVVGPLWSDARLLATAKTISAIIGPLEHPPAQ"
     gene            943..>2219
                     /locus_tag="VFV93_14935"
     CDS             943..>2219
                     /locus_tag="VFV93_14935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5166697.1"
                     /translation="MFDVDAIGDSVVHSRALPAELKKTAANANYPYVSEGLPVFVGSS
                     SLPETGRRTFVIHVAAILALLMTIVYLTWRTFATINPDAWPLAVILLALELHAFVGLV
                     LFTFSLWDVDSVPPARPVDLPPGKVAILIPTYNESMDVLLPTIAAAVSVRVPHETWVL
                     DDGRRPEIRQLATSLGASYLTRDDNQHAKAGNINNALQHIDAEFIAVLDADHVATPDF
                     LSHTLGYFSDPDIALVQTPQDFYNVSSFEHDKLPQNLDKLSAEHRQMYHEQTLFYRVI
                     QPAKNRWGGAFWCGTSSVVRVAALRDIGGVATDTITEDIHTTVRLHRRGWKTVYHNEV
                     LARGLAASSADQYQLQRYRWGTGAMQLLRRENPLFVSGLTFGQRMTYMKTLMGWFDSW
                     RTMGYLLMPPLVLLTGMSPIDAPASQFLTIFLLT"
BASE COUNT          389 a          819 c          636 g          375 t
ORIGIN      
        1 ccccgacctg cccggccagc cacgcggcgc acgctacatg aacagcatcg gcccgctggc
       61 gcgcgacatc gacgacctgg cgctggcgct ctctattatt gaaggccctg acgacttcga
      121 cctcgacgtc ccgccaacac ccgccgacgg gctgccgtcg ctcccggata tggacctgga
      181 cggcgtccgc ttcgccgtcg ccacgaactt ccctggcttg ccggtcgccg ccggcatccg
      241 agcggcgatc gagcgcgtcg ccggcctgat cgagcgcgag ggatgccacg tcgaggagcg
      301 gctgcccgag atcgacttcg acgagcagct tgagacgcgc gcgaccctgc gccgctacgt
      361 gcgcctcttc actgagccgc ccgccgagcc gctgccggct gccgcttact tccacgccct
      421 cgaccgccgc gaccacttca tccgtgcctg gcaggcattc ctcaacgaca cgatcctgat
      481 ctgcccggtc atgatgacga ccgccttccc gcactgcccg cgtgacacgc cggtcacagt
      541 tgacggcgtc gagcatccct acagccagat cgccgactac tgccggccgt tcaacctgac
      601 tggccagccg gtcgtcacga ttccggccgg cctcgacgac gccggcctcc cgatcggtat
      661 ccaggtcgtc ggcccgctct ggtcagatgc gcggctactg gcaaccgcga aaacgatatc
      721 ggcaatcatc ggaccactcg aacatccacc tgcgcaatag cgcccgcaac ggacccaccg
      781 gcataccacc agcggcgatc ggcccgttca aacgggtagc gggaaagaca tttggatacc
      841 tattctccaa gactaatggc tagtcgcaaa cacgactgtt aggtctgcgc ctcagagtca
      901 ccgataacgc accatgcagc agccgttgga aacgtgccaa cgatgttcga tgtggacgcg
      961 attggtgaca gtgtggtgca ttcgcgagca ctgcccgctg agctgaagaa gacagcggcg
     1021 aacgctaact acccgtacgt gagcgagggg ttgccggtct tcgtcgggag ctcgtcgctg
     1081 cccgaaaccg gccgccgcac gttcgtgatc cacgtcgcgg caatcctcgc gctcctgatg
     1141 acgatcgtct atctgacctg gcgcaccttc gcgacgatca acccggatgc ctggccactg
     1201 gcggtcatcc tgctcgcgct ggagctgcac gccttcgtcg gactggtgct cttcaccttc
     1261 tcgctctggg atgtcgacag cgttccaccg gcccggccgg tcgatctccc gcccggcaag
     1321 gtcgcgatcc tgatcccgac ctacaatgag agcatggatg tcctgctgcc gacgatcgcg
     1381 gcagccgtct ccgttcgtgt tccgcacgag acgtgggtgc tcgacgatgg ccgccggccg
     1441 gagatccgcc agctggcgac ctcgctcggc gcctcctatc tcacccgcga cgacaaccag
     1501 cacgccaagg ccggcaacat caacaacgcg ctccagcaca tcgacgccga gttcatcgcc
     1561 gtcctcgatg ccgaccacgt cgcaacgccc gacttcctca gccacacgct tggctacttc
     1621 tcggaccccg acatcgcgct cgtccagacg ccgcaggact tctacaacgt ctcctcattc
     1681 gagcacgaca agctgccaca gaacctcgac aagctctcgg ctgagcatcg ccagatgtac
     1741 cacgagcaga cactcttcta ccgcgtcatc cagccggcca agaaccgctg gggcggcgcg
     1801 ttctggtgtg gcaccagctc ggtcgtccgt gtcgccgccc tgcgcgacat cggtggcgtc
     1861 gcaaccgata cgatcaccga ggacatccac acgactgtcc gtctccaccg gcgcggctgg
     1921 aagaccgtct accacaacga ggtgctggca cgcggcctgg ccgccagcag cgccgaccag
     1981 taccagctcc agcgctaccg ctggggaacg ggcgcgatgc agctgctgcg gcgggagaac
     2041 ccgctcttcg tctccggcct gaccttcggc cagcgcatga cctacatgaa gacgctgatg
     2101 ggctggttcg actcgtggcg gacgatgggc tacctgctga tgccgccgct cgtcctgctg
     2161 accggcatgt caccgatcga cgctccggcc agccagttcc tgacgatctt cctgctgac
//
LOCUS       DASUGJ010000655         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_384928, whole genome shotgun sequence.
ACCESSION   DASUGJ010000655 DASUGJ010000000
VERSION     DASUGJ010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384928"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..264)
                     /locus_tag="VFV93_14940"
     CDS             complement(28..264)
                     /locus_tag="VFV93_14940"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HEX5166698.1"
                     /translation="MSDRRRLTVDEIELNADNERVAVLIDDDGSQLVLPLALLPEGTR
                     VGDVLNLSLAPDADETQRRRKRVKDLQKKLFGDR"
     gene            complement(261..1244)
                     /locus_tag="VFV93_14945"
     CDS             complement(261..1244)
                     /locus_tag="VFV93_14945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX5166699.1"
                     /translation="MQSQDNASAARTCLILTAIGAAFVLIVCLVVGLGLIIDFGGDDH
                     QPEDQRGTVVADDADEQPVLFSVMDVGQGACVVVITPDGKVLVADAGRSQERVRRVVI
                     PYLREHGVERIDYVVATNPDQDHIGGLPALLDEFEVGAWVDPVVETTNQTYAALLEQV
                     DQLGIEPIRARRGGTLDMGPRLTAEILWPVDPLMMDGSEESHNDNSIVIKLVDGDVSF
                     LITGDIEAPAEEALVEEDDDEQLRADVMVVPHHGSNTSSTAEFLDAISPTVAIIPVGL
                     DNQYGHPRDETLQRLRFRGIRVYRTDLDGTVEVRSNGDEYQVEVLGTEDGS"
     gene            complement(1266..1862)
                     /locus_tag="VFV93_14950"
     CDS             complement(1266..1862)
                     /locus_tag="VFV93_14950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5166700.1"
                     /translation="MVSTRFANRVRGRLGPLGTAVARTHLSPNMLTVLGLLLNAGVAA
                     VIASGNLVLGGVLVLVAGGFDALDGAVARATGQSSRFGGFLDSTIDRYSEAVVFAGLL
                     IYLTETDAGTIPILLTYATIVGSLMISYSRAKAEQLGIKGDVGFAQRLERVIILAFFL
                     LISEPVWGLWILAIATHLTALHRVLHVWNVTRQDGARS"
     gene            complement(1855..>2596)
                     /locus_tag="VFV93_14955"
     CDS             complement(1855..>2596)
                     /locus_tag="VFV93_14955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5166701.1"
                     /translation="DAKIAVSEAARAFVDGFFPAEFEIVPNGIALDDYTQHVEPLPEL
                     MDGRRNILFVGRYNEPRKGFRYLVRAMPMIRGQFPDARLVVVGNGDPSRYERFLEQHG
                     VTDVIFAGFVPYEMLPRYYASADVFCAPSTGRESFGLVLLEGLASGVPVVAAANAGYS
                     AVIDNGVNGVLTPPKDEHALAMGIVRVLADTEFRQRIVRNGLEHVKQFDWPVVARQVL
                     DVYERAIVKSAAGPVQRVWPVEIAGRRG"
BASE COUNT          446 a          945 c          786 g          419 t
ORIGIN      
        1 gaactacccg atgctttcaa agccagctca ccgatccccg aacagcttct tctgcaggtc
       61 cttgacccgc ttgcgacgcc gttgggtctc gtcggcgtcg ggggcgagcg agaggttcag
      121 cacatcccca acccgcgttc cctccggcaa cagcgccagc ggcaacacga gttgggagcc
      181 gtcgtcatcg atcaggaccg cgacccgctc gttgtcggcg tttagctcga tctcatcgac
      241 ggtcagccgg cgtctgtcgc tcacgatccg tcctccgtcc cgagtacctc gacctgatac
      301 tcgtcgccgt tgctgcggac ctcgaccgtc ccgtccaggt cggtccggta gacgcggatc
      361 ccgcgaaagc gcagccgctg gagcgtctcg tcgcgcggat gcccgtactg gttgtcgagt
      421 ccgaccggga tgatcgccac cgtcggtgag atcgcgtcga ggaactcagc cgtgctcgac
      481 gtgttgctgc cgtgatgcgg gacgaccatt acatccgccc gcagttgctc gtcgtcatcc
      541 tcctcgacca gtgcctcctc ggccggagct tcgatgtcgc cggtgatcag gaacgagaca
      601 tcgccatcga ccagcttgat gacgatgctg ttgtcgttgt ggctctcctc cgagccgtcc
      661 atcatcagcg gatcgaccgg ccagaggatc tcggccgtta gccgcgggcc catatccagc
      721 gtcccacccc tccgcgcgcg gatcggctcg atccccaact ggtcgacctg ctccagcagc
      781 gccgcgtagg tctggttggt cgtctcaacg accgggtcga cccaggctcc gacctcgaac
      841 tcgtccagca gcgccggcag cccgccgatg tggtcctgat cgggattggt cgcaaccacg
      901 tagtcgatcc gctcgacccc gtgctcgcgc aggtacggga tcaccacccg ccggacccgc
      961 tcctgcgagc gaccggcgtc ggcgaccagg accttgccgt ccggcgtgat gacgacgaca
     1021 caggcgccct ggccaacgtc catgacactg aacagcaccg gctgttcgtc cgcgtcgtcc
     1081 gcgacgactg tcccgcgttg gtcttccggc tggtggtcgt cgccgccgaa gtcgatgatc
     1141 agtcccagcc cgacgaccag acagacgatc agcacgaacg ccgcgccgat cgccgtcagg
     1201 atcaggcatg ttcgcgccgc cgaggcatta tcctgactct gcaaaccact tcctgtacgt
     1261 gtgaattacg accgcgcccc gtcctgccgg gtcacgttcc agacatgcag cacacggtgg
     1321 agcgccgtga gatgggtcgc gatcgccagg atccacaacc cccagaccgg ctccgaaatc
     1381 agtagaaaga acgccagaat gatcacgcgc tccagtcgct gggcgaaacc gacatcaccc
     1441 ttgatcccca gttgctcggc cttggcgcgg ctgtacgaga tcatcagcga gccgacgatc
     1501 gtcgcgtacg tcagcaggat cggaatcgtc cccgcgtcag tctcggtcag gtagatcagc
     1561 agcccggcga agacgaccgc ctccgagtag cggtcgatgg tgctgtcgag gaacccgcca
     1621 aagcggctgg attgccccgt cgcccgcgcc accgcgccat ccagcgcgtc gaatccgccc
     1681 gccaccagca ccagcacccc gcccagcacc aggtttccgg acgcgatcac ggccgcgacc
     1741 ccggcattga gcagcagacc cagcaccgtc agcatgttcg gcgagaggtg cgtccgggcg
     1801 acggctgtcc cgagcggccc cagccggccg cgcacccggt tggcgaagcg agtgctaacc
     1861 acgtcggccc gcgatctcga ccggccagac gcgctggacc ggcccggccg cgctcttgac
     1921 gatcgcacgc tcgtagacat ccagcacctg ccgtgcgaca accggccagt caaactgctt
     1981 gacatgctcc agcccgttgc gcacgatccg ctgacggaat tcggtgtcag ccagcacccg
     2041 gacgatcccc atcgccagcg cgtgctcgtc cttgggcggc gtcaggaccc cgttgacgcc
     2101 gttgtcgatg acggcgctgt agccggcgtt ggcggccgcg acgaccggca caccgctcgc
     2161 cagcccctcc agcaggacca gcccgaacga ctcccgcccg gtcgacggcg cgcagaacac
     2221 gtccgccgag gcgtaatagc gcggcaacat ctcgtacggc acgaagccgg cgaagatcac
     2281 gtcagtaacg ccgtgctgct ccaggaagcg ctcgtagcgc gatgggtcgc cgttgccgac
     2341 caccaccagc cgcgcgtcgg ggaactggcc gcgaatcatc ggcatcgccc gcaccaggta
     2401 gcggaacccc ttgcgcggct cgttgtagcg cccgacgaag aggatgttgc gtcgcccgtc
     2461 catcagttcc ggcagcggct cgacgtgctg ggtgtagtcg tccagcgcga tcccgttggg
     2521 cacgatctcg aactcggccg ggaagaaacc atcgacaaac gcacgcgccg cctccgagac
     2581 ggcgatcttg gcgtcc
//
LOCUS       DASUGJ010000656         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2052855, whole genome shotgun sequence.
ACCESSION   DASUGJ010000656 DASUGJ010000000
VERSION     DASUGJ010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052855"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFV93_14960"
     CDS             <1..257
                     /locus_tag="VFV93_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5166702.1"
                     /translation="LAVDVVKDVHRLNPRRMFNALIPRSIRLAEAPSHGMSIFEYDPT
                     SRGAEAYRLLGAEVVQRLGLDSDQPSTFGQQPAENDRRTA"
     gene            364..1905
                     /gene="guaB"
                     /locus_tag="VFV93_14965"
     CDS             364..1905
                     /gene="guaB"
                     /locus_tag="VFV93_14965"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5166703.1"
                     /translation="MSILGDPQAKRVAGAESYGADGVVSDKVIGTGLTFDDVLLLPAQ
                     SDLIPSQADTSAWFTPNIRLKIPIVSSPMDTVTEARMAIALAREGGIGVIHRNLSIED
                     QVAEVDKVKRSESGMIVEPVTLGPDNMLADAEAVMNRYRISGVPITTPDGKLVGILTN
                     RDLRFEDDLNQPIHEFMTSENIVTVPVGTTLDQAREVLHKYKVEKLPVVDDHGYLKGL
                     ITVKDIQKKIQYPNATTDEQGRLRVAAAVGVGAEAMARCEALITANVDVIIVDTSHGH
                     SRGVLNMVRDVKSTWNIEVVAGNVATGDATGALIEMGADAVKVGVGPGSICTTRVVAG
                     TGVPQISAIMDCVRVANSTGIPVIADGGIQYSGDIAKAIAAGADCVMLGSLFAGTDES
                     PGEVILYQGERYKEYRGMGSLGAMKTRSFSKDRYFQQDVDSLAKFVPEGIEGRVAYKG
                     QTSNVIYQLVGGLRQAMGYCGAATIDDLKTNARFVRITSAGLRESHPHDVVITKEAPN
                     YRASY"
BASE COUNT          423 a          708 c          674 g          349 t
ORIGIN      
        1 ggctcgcggt cgatgtcgtc aaggatgtcc atcgcctcaa cccgcggcgg atgttcaacg
       61 cactcatccc gcgctcgatc cggcttgccg aggcgccatc gcacggcatg tcgatcttcg
      121 agtatgaccc cacttctcgc ggcgccgaag cctaccgcct gctcggcgcc gaagtcgttc
      181 agcggctggg gctggacagt gaccagccat caacgttcgg ccagcagccg gcagagaacg
      241 acaggcggac ggcgtagtgt cgtgcgtagt gcgcgtcgaa cgagcacatg cagggggaaa
      301 tcgtcatcat cgcaacatgc cggcggctga tcgctgccgg cgactcgcca caaggaggaa
      361 acagtgagca ttctcggaga tccgcaggcg aaaagggttg ccggtgcgga gagttacgga
      421 gctgacggtg tcgtcagcga caaagtgatc ggcaccggcc tgacgttcga cgacgtgctg
      481 ctcctgccgg cacagtcgga cctgatcccc agtcaggccg ataccagcgc ctggttcacg
      541 cccaatatcc gtctgaagat cccgatcgtt tcgtcaccga tggacaccgt caccgaggcg
      601 cggatggcga tcgccctggc ccgcgagggg ggtatcggtg tcatccaccg caacctctcg
      661 atcgaggatc aggtggccga ggtcgacaag gtcaagcgct ccgaatcggg catgatcgtc
      721 gagccggtca cgctcggccc cgacaatatg ctcgccgacg ccgaagccgt catgaaccgc
      781 taccgcatct cgggcgtacc gatcacgacg cccgacggga agctggttgg aatcctgacc
      841 aaccgcgacc tgcgcttcga ggacgacctc aaccagccga tccacgagtt catgaccagc
      901 gagaacattg tcaccgtccc ggtcggaacg acgcttgacc aggcccgcga agtgctccac
      961 aagtacaagg ttgaaaagct gccggttgtt gacgaccacg gctatctcaa gggtctgatc
     1021 accgttaaag acattcagaa gaagatccag tacccgaacg cgacgactga tgagcagggt
     1081 cgcctgcggg ttgccgccgc cgtcggggtc ggcgccgaag cgatggcacg ctgcgaggca
     1141 ctgatcacgg ccaacgtcga tgtcattatc gttgacacgt cccacggtca ctcacgcggc
     1201 gtcctcaaca tggttcgtga cgtgaagagc acctggaaca tcgaggtcgt ggccggcaat
     1261 gtcgcgaccg gtgacgcaac cggggcgctg atcgagatgg gcgccgacgc ggtcaaggtc
     1321 ggcgttggcc ctggctcgat ctgcacgacc cgtgtcgtcg ccggcaccgg cgtcccgcag
     1381 atctcggcaa tcatggactg cgtccgcgtt gcaaactcga ccggtatccc ggtcatcgcc
     1441 gacggcggca tccagtattc cggcgacatc gcgaaggcga tcgctgccgg cgctgactgc
     1501 gtgatgctcg gcagcctctt cgccggcact gacgagagcc ccggcgaggt gatcctgtac
     1561 cagggcgagc gctacaaaga gtatcgcggg atgggatcgc tcggcgcgat gaagacccgc
     1621 tcgttctcca aggaccgcta cttccagcag gatgtcgaca gcctggccaa gttcgtccct
     1681 gagggcatcg aggggcgcgt cgcctacaaa ggtcagacct ccaacgtcat ctatcagctt
     1741 gtggggggtc tccgacaggc gatgggctac tgcggcgcgg ccacgatcga cgacctcaag
     1801 accaacgccc gcttcgtccg catcacctcc gccggtctcc gcgaaagcca cccccacgac
     1861 gtcgtcatca ccaaggaagc tccgaactac cgcgcgtcgt attagtcgcc gcacgaacca
     1921 aaagccccct ctccgcatcg gggagggggt tgggggaggg gttttggaca cgcaccacgc
     1981 gagcgaggac cgatgtcctc agtcgtctcc aacaaccccc agcaacgcaa acgccagcgg
     2041 caagccaaga tcgccccggt cgctgttagt gagcgtgacg gtcgtgaccc gcttgccggc
     2101 gaggttggga acatgcagcg caccggccga gtagcccggc cctccgccgc cgtg
//
LOCUS       DASUGJ010000657         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1775057, whole genome shotgun sequence.
ACCESSION   DASUGJ010000657 DASUGJ010000000
VERSION     DASUGJ010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775057"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..837
                     /gene="fliP"
                     /locus_tag="VFV93_14970"
     CDS             169..837
                     /gene="fliP"
                     /locus_tag="VFV93_14970"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX5166704.1"
                     /translation="MNPNNLTVNVDGIGAMSAPLQIVLLLTLISFLPAIIVTMTSFTR
                     IAIVFHFLRQALGTQEMPSNQILIGLALFLTMFIMAPVASEISAKAVQPAMAGTLTVT
                     EALEQGTPPLRTFMLKQTREKDLELFMSLGKVEKPKTKDGLPMTVVIPAYIISELKTG
                     FQMGFFLFVPFLLIDLVVSTTLLSMGMLQLPPAMVSLPFKVLLFVMIDGWNLLVGSIV
                     RSFA"
     gene            868..1110
                     /locus_tag="VFV93_14975"
     CDS             868..1110
                     /locus_tag="VFV93_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HEX5166705.1"
                     /translation="MRQAIETAIVVSLPMLLAGLVAGVLVSIFQTVTSIQDNVLAFIP
                     RAAAIFAVFALTFPWILRILAGFATNLITRLPEYVR"
     gene            1110..1862
                     /locus_tag="VFV93_14980"
     CDS             1110..1862
                     /locus_tag="VFV93_14980"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX5166706.1"
                     /translation="MDLTPVLRFALLLVRPGVVVMLAPGLGGRQIPAMAKIALTVMAA
                     VALMPTVQLPETVGSNLVLIILREAAVGLSLAFVLQVLIAGAEFAGHLSSYQIGFSYG
                     ATIDPISGVRSTMLVSLYGMLATLVFLGVNGHHALLRALAASYDGVPIGVVQVNGSLV
                     SAVRDMLAMVFTVGLRLAAPVLIVLLIVEMAVGLISRAAPSLSFMVIGYPIRIVVGLF
                     LVAALIATVPGVIESMIERTLMLGGRTATAFR"
     gene            1866..>2692
                     /locus_tag="VFV93_14985"
     CDS             1866..>2692
                     /locus_tag="VFV93_14985"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX5166707.1"
                     /translation="MSQERSEHPSAKRLRDARRKGQFVHSRDLAIAGASVAATMALAG
                     LGAYVFQGLASRLTQDLGQLGADPLRTVTTGELTALVVGGGRLIAVLVGPVAMCTVGA
                     AVMIHGFQGGWSLAPEALQFNWSRLSPANGIKRFGLMQSGMDTLKTLISVAAISVIAW
                     MSVDAMFDDAARLPWLSPLAAAAVGWQHTQTLLWRVAWALGFLAIGDYGLQYYRTMAQ
                     LKMTKQELRDELKENEGNPEVKGRVRRLQREMVKRRMLSDVKRATVVITNPTHFAVAL
                     "
BASE COUNT          403 a          870 c          891 g          528 t
ORIGIN      
        1 ccgatcgctg gtcgtcgtgg cggtggaagg ccgccggctg ctgctcggcc tcacgccggc
       61 cagcgtgtcg atgctcgtcg aactgaatcc ggtggctccc ggtcaggatg cgactcaggg
      121 tgccaccccc gtactggcgc cgttcgagca ggaactgaac aagcggacat gaatccgaac
      181 aacctcacgg tcaacgtcga cggaatcggc gcgatgtcgg cgccgctgca gatcgtcctg
      241 ctgctgacgc tgatctcgtt tctgccggcc atcatcgtga cgatgacgtc gttcacgcgc
      301 atcgccatcg tctttcactt cctgcggcag gcgctcggca cgcaggagat gccgtcgaac
      361 cagatcctta tcgggctggc gctcttcctg acgatgttca tcatggcgcc ggtagcctcg
      421 gagatctccg ccaaagccgt tcagccggcg atggccggca cgctgaccgt gaccgaagcg
      481 ctcgagcagg ggacgccgcc gctgcgcacc ttcatgctga agcagacgcg cgagaaggac
      541 ctcgagctgt tcatgtcgct cggcaaggtg gagaagccga agaccaagga cggcctgccg
      601 atgaccgtcg tcattccggc ctacatcatc tccgagctga agaccggctt ccagatgggg
      661 ttcttcctgt tcgtcccgtt cctgctgatc gaccttgtcg tgtcgacgac gctgctgtcg
      721 atgggcatgc tgcagctgcc gccggcgatg gtgtcgctgc cgttcaaggt attgctgttc
      781 gtgatgatcg acgggtggaa tctgctggtc ggatcgatcg tgcggagctt cgcgtagccg
      841 atgtcggaag cgctcgtcgt cggtgtcgtg cggcaggcga ttgaaaccgc catcgtcgtc
      901 agtctgccga tgctcctggc cggcctggtg gctggcgtcc tcgtgagcat cttccagacg
      961 gtgacgtcga tccaggacaa cgtgctcgcc ttcattccgc gcgccgccgc gatctttgcg
     1021 gtgttcgcgc tgacgttccc ctggatcctg cgcatcctgg cgggctttgc caccaacctg
     1081 atcacccggc ttcccgagta cgtccgctga tggacctgac gccggtactg cgattcgctc
     1141 tgctgctggt gcgacccgga gtggtcgtca tgctcgctcc gggtctgggc gggcgccaga
     1201 ttccggcgat ggccaagatt gcgctgacgg tcatggccgc cgtcgcgctg atgccgaccg
     1261 tgcagctgcc cgaaacggtc ggctcgaacc tggtgctgat cattctccgg gaggcggcgg
     1321 tcggtctgtc actcgcgttc gtcctgcagg tgctgattgc cggtgccgag ttcgccggcc
     1381 acttgagcag ctatcagatc ggcttctcgt acggcgccac gatcgatccg atcagcggtg
     1441 tgcgcagcac gatgctggtc tcgttgtacg ggatgctggc gacgctggtc ttcctcggcg
     1501 tcaacggcca ccatgcgctg ctgcgcgcgc tggccgcgtc ctatgacggc gtgccgatcg
     1561 gcgtggtgca ggtgaacggg tcgctggtca gcgcggtgcg cgacatgctg gcgatggtct
     1621 tcaccgtcgg cctgcgactg gccgcaccgg tgctcatcgt tcttctcatc gtcgaaatgg
     1681 cggttggctt gatttcccgc gcggcgccgt cactgagctt catggtgatc ggctacccga
     1741 tccggatcgt cgtcgggctg ttcctggtcg ccgcgctgat tgccacggtg cccggggtga
     1801 tcgagtcgat gatcgagcgc accttgatgc tgggagggcg gaccgccacg gcgttccggt
     1861 agcccatgtc ccaggagcgt tcggaacacc cctcagccaa acgccttcga gacgcccgcc
     1921 gcaaaggtca gttcgtccac agccgcgacc tggcgatcgc cggcgcctcc gtggcggcga
     1981 cgatggcgct ggccggcctt ggcgcatacg tctttcaggg gctcgccagc agactgacgc
     2041 aggacctcgg gcagctgggt gccgatcccc tgcggacagt cacgacgggg gagctcaccg
     2101 cgctggtggt cggcggcgga cgtctgatcg ccgtgctcgt tggtccggtg gcgatgtgca
     2161 ctgtcggcgc cgccgtcatg attcacgggt tccagggcgg gtggtcactg gcgccggagg
     2221 ccctgcaatt caactggtcg cgactgagtc ccgccaacgg catcaagcga ttcggactga
     2281 tgcagtccgg catggacacg ctgaagacgc tcatctccgt cgccgccatc tcggtcatcg
     2341 cctggatgag cgtggacgcg atgttcgacg acgccgcgcg actgccgtgg ctgtcgccgt
     2401 tggccgccgc ggccgtgggc tggcagcaca cccagaccct gttatggcgc gtcgcctggg
     2461 cgctcggatt ccttgcgatc ggcgactacg gccttcagta ctaccgcacg atggcgcagc
     2521 tcaagatgac caagcaggag ctgcgggacg aactgaaaga gaacgaaggc aatcccgagg
     2581 tgaagggacg cgttcgccgc ctgcagcgcg agatggtcaa acgccgcatg ctgagcgacg
     2641 tcaaacgcgc gacggtggtc atcaccaacc cgacacactt tgccgtcgcc ct
//
LOCUS       DASUGJ010000658         6587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1357736, whole genome shotgun sequence.
ACCESSION   DASUGJ010000658 DASUGJ010000000
VERSION     DASUGJ010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357736"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1041
                     /locus_tag="VFV93_14990"
     CDS             49..1041
                     /locus_tag="VFV93_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166708.1"
                     /translation="MRRLFFLVFVLIALSLVTFVLMHVVPSDPARNIAGPRASPAAVQ
                     QIREDYGLDDPIVERYVNYVKGVVQLDLGMSLSSRRPVTQDLRRYLPATIELALAGML
                     LSLIVGVPLGVISAARKNSAIDAFGRVVSVIGLSMPSFWLAIILQFIFFARLGWLPDG
                     ERLPVGVDPPRTITTLYTVDALLTLNFDVFLTALKHLAMPAFVMGFISLAPVTRMVRS
                     SMLEVLNQDYIRTARAKGLTQRLVISRHALKNALLPTVTVLGLQLGLLLSGTVLVEIV
                     FSWPGIGRYAFQSIQNQDPNALISVTLVIGIGYVLANLLVDIAYSFLDPRIRVT"
     gene            1044..1967
                     /gene="nikC"
                     /locus_tag="VFV93_14995"
     CDS             1044..1967
                     /gene="nikC"
                     /locus_tag="VFV93_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HEX5166709.1"
                     /translation="MQSTDTTFESAAGASPPRAEQLAPANRSGLRYFIRRFSRESPLN
                     LIALGIILVFLLMALLGPTIAPYDPIKTNAPNRLADPSLSHPFGTDELGRDLFSRVIV
                     GARISLGVATLILAIAVPVGTVIGLAAGYAGGIIDELLMRLTDIFLAFPAIILAMAIA
                     AALGPNLRNTIIALTLVYWPWYARLVRGQVLQVKERDYIEAARSVGVSPARLVRRHIL
                     PNSVTPIIIQATIDIGFAVLATAGLSFIGLGAQPPTAEWGAMISTARGFFRVAWWYFT
                     FPGVALMITVVGFNLLGDGLRDYFDPRTRGS"
     gene            2078..3910
                     /locus_tag="VFV93_15000"
     CDS             2078..3910
                     /locus_tag="VFV93_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5166710.1"
                     /translation="MADQQDGAFLDLIVGARLGRLTRRQVLQRGLALGLSTPVIAGLL
                     SACGGDDDDDDDDGGTTPEASGGNATAETTATRVPVVAATRTPDTAASPSATEGSGTS
                     GRNESDQKTFTMAFTGGVPDVDPQSAYDNQASSMFMAVYEMLLRLDGESTFEYQPMLA
                     NAWEASDDLTEFTFTIDEGITFHDGSACDAQAIVDSLTRFVEMSRGPVDVLKRFVPDP
                     ATQMEAVDATTIKFTMKKPEPLFLAAMASEYGPLIVSPAAMEANKTADDPYAHEWFSQ
                     NMVGTGPYKVTEAEPQERFVLDRYEGYAHGPVHFFDQIVARVVLEDVTRRQLIESGDV
                     DGVAVLPSDDVIDLRDNDDVQVIEYDTTECDWIRLNYVRLSAEARQGLCYAFPYADVI
                     DQVLQGFAKEQGPIADTVIGFDPSIPLYSTDLAKAAELLEAGGVSPSDELEYMYADGD
                     TDDEAIAQLFQASLAEAGYTLKLTKVDRSALIDMNYGDSPPEERPHMTSSGWWPDYND
                     SYNQLFPNFHPDSAGSAGSNSMFYGNEEVGALLDQARDAATEEELVEATGKILQIMIN
                     DDPAAVFYAQITRVQLLRHDIRDFKPNGIYIASYNFHEMWREAT"
     gene            complement(4002..5246)
                     /locus_tag="VFV93_15005"
     CDS             complement(4002..5246)
                     /locus_tag="VFV93_15005"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX5166711.1"
                     /translation="MASIDWSKTQFETRAIHDGQEPEETTGAVIVPIYQTSTFAQTGI
                     GQHKGFEYARTDNPTRTALQTAIASLEGGKWGLAYGSGMAAIQNLLYMLVPGDHVLMS
                     DDAYGGTFRLIDKIFARYGIEYSMVDLSDLEQVREGLRDDTRMVISETPTNPYLKIVD
                     IGGISELLRERYGAPSGEAISWESGTRGFDLTRPLLLVDNTFASPYLQNPLAHGADLV
                     VHSATKYLGGHSDVVLGLVAGNDESVLRFLRFHQNAAGAVPGPLDCWLILRGLKTLGI
                     RMREHSANAMRIAEWLQQDPGVKDVYYPGLASHPNHDVAARQMRAFSGMVSFTVHGGW
                     ESASRVVAATRIFTLAESLGGVESLIEHPGQMTHLSTAGSKAEVGEDLIRLSVGIEHV
                     DDLIADLEQALEVATPARATAD"
     gene            complement(5362..>6587)
                     /locus_tag="VFV93_15010"
     CDS             complement(5362..>6587)
                     /locus_tag="VFV93_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5166712.1"
                     /translation="IKPGGTIVEPTSGNTGVGLAMAAAIKGYRCVFVMPDKVSGEKIA
                     LLRAYGAEVVTTPTNVERESPESYYSVADRLTRELPNAFQPNQYFNPMNPRTHYESTG
                     PEIWQGTDGKVTHFVAGVGTGGTISGISKFLKEQNPDIRVIGADPEGSIYTGPVPRPY
                     KVEGVGEDFWPGTFDRATVDEFIMVTDRDSFAAARRVTREEGILIGGSGGLAVWAALE
                     VAKREGPEALVVVLLPDSGRGYLSKLYNDDWMRENGFLSRFSQAPRAGDLVSSYHDDD
                     VPKIVAVHVDDTVERAIALLQEFNISQLPVVRTAGGQSNGAIEVHNIAGSIQERTLLD
                     KVFRSPETLRAKISTVMDAPFPLVDAAEEIERVFPLLSTAGASAVLVQRTGALVGIIT
                     RADLLDFVANMKGDG"
BASE COUNT         1102 a         2236 c         2061 g         1188 t
ORIGIN      
        1 tcgccgcgac ctgagtgatt cgaggtcagg atgattgctc gcttgattgt gcgacggctg
       61 tttttccttg tgtttgtgct gattgcgttg tcactggtga cgttcgtgct gatgcacgtc
      121 gtccccagcg atccggcccg caacattgcc gggccgcgcg ccagtccggc ggcagtccag
      181 cagatccgcg aggactacgg actcgacgat ccaatcgttg aacgctacgt caactacgtc
      241 aaaggcgtcg tccaactcga cctcggtatg tcgctctcct cgcgccggcc ggtgacccag
      301 gacctgcgac gctacctgcc ggccacgatc gagctcgcgc tcgccggcat gctgctctcg
      361 ctgatcgtcg gtgtcccgct cggcgtcatc tcggcggcgc gcaagaactc ggcgatcgac
      421 gccttcggcc gggtcgtctc cgtcatcggc ctgtcgatgc catcgttctg gctggcgatc
      481 atcctccagt tcatcttctt cgcgcggctc ggctggctgc ccgatggcga gcggttgccg
      541 gtcggtgtcg acccgccacg aacgatcacg acgctctaca ctgtcgacgc gctgctgacg
      601 ctgaacttcg atgtttttct gaccgctctc aagcacctgg cgatgccggc cttcgtgatg
      661 ggcttcatct cgctggcacc cgtgaccaga atggtgcgct ccagcatgct cgaagtcctg
      721 aaccaggatt acatccggac cgcccgcgcg aaggggctga cccagcgcct cgtgatctcc
      781 cggcacgctc tcaagaacgc gctcctgcca accgtcacgg ttctcggcct gcaactcggg
      841 ctgctgctga gtggaacggt gctggtcgag atcgtcttct catggcccgg catcggacgc
      901 tacgcgttcc agtcgatcca gaatcaggat ccgaacgcgc tgatctcggt gacgctggtg
      961 atcggcatcg gctatgtcct cgccaacctg ctggttgaca tcgcctactc gttcctcgat
     1021 ccaagaatcc gggtgaccta gccatgcaga gcacagacac gacattcgaa tccgccgccg
     1081 gcgcgagtcc accccgggcc gagcagctcg cgccggccaa ccgcagcggc ctgcgctact
     1141 tcatccggcg tttctcccga gagagcccgc tcaatctgat cgcgctcggc attatcctcg
     1201 tcttcctgct gatggccctg cttggcccga cgatcgcgcc ctacgatccg atcaagacca
     1261 acgcgccgaa ccggctggca gatccgtcgc tctcgcaccc gtttggaacc gatgagcttg
     1321 gccgcgacct gttcagccgc gtcatcgtcg gcgctcgcat ctcgctcggt gtcgcgaccc
     1381 tgattctcgc gatcgcagtt ccggtcggga ccgtgatcgg actcgccgcc ggctacgccg
     1441 gcgggatcat cgacgagttg ctgatgcggc tcaccgacat cttcctggca ttcccggcca
     1501 tcatcttggc gatggcgatc gccgctgcgc taggaccgaa cctgcgcaac accatcatcg
     1561 cgctgacgct ggtctactgg ccgtggtatg cccggctggt gcgcgggcag gtgctgcagg
     1621 tcaaggaacg tgactacatc gaggccgcgc gctcggtcgg cgtgtcgccg gcgcggctcg
     1681 tccggcggca cattctgccc aacagcgtca cgccgatcat catccaggcc acgatcgaca
     1741 tcggcttcgc ggtcctggca acggccgggc tgtcattcat cggcctcggc gcgcagccgc
     1801 cgacggcgga gtggggcgcg atgatctcga cggcgcgcgg cttcttccgc gtcgcctggt
     1861 ggtacttcac cttccccggc gtggcgctga tgatcaccgt cgtcggcttc aacctgctgg
     1921 gtgacggcct gcgtgactac ttcgatccac gtacacgcgg cagctaaccc tggatgctgc
     1981 atcaccggac gaccggagga ggtggaaccg gcgcgaacct tccgacgatg gctgagaacc
     2041 cctactgttg ccatgatggg acatacgagg aggaacaatg gcggaccagc aggacggtgc
     2101 ttttctcgat ttgatcgttg gagcgagact cggccggctg acgcgccggc aggtgctgca
     2161 acgcggcctc gcgctcgggt tgtcgacgcc ggtcatcgcg ggactactct ccgcctgcgg
     2221 cggcgacgat gatgacgacg atgacgacgg cggcaccact cccgaagcca gcggcggcaa
     2281 cgccacagct gagacgaccg cgacccgtgt gcccgttgtc gcggcaacac gcacacccga
     2341 cactgccgcg tcaccgtcag cgactgaggg gtccggaacg tccggccgca acgaatcgga
     2401 ccagaagacg ttcacgatgg cgtttaccgg cggcgtgccg gatgtcgatc cgcaatccgc
     2461 ctacgacaac caggccagca gcatgttcat ggcggtttac gagatgctgt tgcggctgga
     2521 tggcgagagc accttcgagt accagccgat gctcgccaac gcctgggaag ccagcgatga
     2581 cctgaccgag ttcaccttca cgatcgacga gggcatcacc ttccacgacg gctcggcctg
     2641 cgatgcgcag gcgatcgtcg actcgttgac ccgcttcgtc gaaatgagcc gcggcccggt
     2701 cgacgtcctc aagcgcttcg tgccggaccc agccacccag atggaagccg tcgatgccac
     2761 gacgatcaag ttcacgatga agaagccgga gccgctcttc ctggccgcga tggcgtcgga
     2821 gtacggcccg ctgatcgtct cgccggccgc aatggaggcc aacaagacgg ccgacgatcc
     2881 atacgcgcac gagtggttct cgcagaacat ggtcggaacc ggcccgtaca aggtcaccga
     2941 ggccgagccg caggagcgct tcgtgctgga tcgctatgag ggctatgcgc atggcccggt
     3001 gcacttcttc gaccagatcg tcgcccgcgt cgtcctcgaa gatgtgacgc ggcggcaact
     3061 gatcgagtcc ggcgatgtcg atggcgtcgc ggtcctgccg tctgacgacg tgatcgatct
     3121 gcgcgacaac gatgacgtgc aagtcatcga gtacgacacg accgagtgcg actggatccg
     3181 cctgaactat gtgcgactca gtgcggaagc ccgccagggg ctgtgctacg cgttcccgta
     3241 cgccgacgtg atcgaccagg tgctccaggg cttcgccaag gagcaggggc cgatcgccga
     3301 taccgtgatc ggctttgacc cgtcgatccc gctttactcc accgatctcg ccaaggccgc
     3361 agagctgctg gaggccggcg gagtcagccc gtccgacgag ttggagtaca tgtacgccga
     3421 cggagacacc gacgacgagg cgattgccca gctcttccag gccagcctcg ccgaagccgg
     3481 ctatacgctc aagctgacca aggtcgaccg ctcggcgctg atcgacatga actacggcga
     3541 ttcgccgccg gaagagcgcc cccacatgac atcgagcggt tggtggcctg actacaatga
     3601 ctcctacaac cagctcttcc cgaacttcca tccggattca gcgggcagcg ccggatcaaa
     3661 ctcaatgttc tacggcaacg aagaagtcgg tgcgttgctg gatcaggccc gcgacgccgc
     3721 gaccgaggaa gagctggtcg aagcgaccgg caagatcctc cagatcatga tcaacgacga
     3781 tccggctgcc gtcttctatg cgcagatcac ccgcgtccag ctgctacgcc acgacatccg
     3841 cgacttcaag cccaacggca tctacatcgc ctcctacaac ttccacgaga tgtggcggga
     3901 ggcgacgtag gacggcggga ttcgggactc gggattcggg ggtattgaac ggggcgattt
     3961 ccttcgacgc tcccggttcc cgagtcccgt gtcccgccgg tctaatccgc cgtcgcccga
     4021 gccggcgtgg cgacttcaag cgcctgctcc aggtcggcga tcaggtcatc gacgtgctcg
     4081 atgccgactg agaggcggat caggtcctcg ccgacctcgg ccttcgatcc ggctgtgctg
     4141 aggtgcgtca tctggccggg gtgctcgatc aacgactcga caccgcccag gctctcggcc
     4201 agcgtgaaga tcctggttgc cgcgacgacc cggctggccg actcccagcc gccatggacg
     4261 gtgaacgaga ccatccccga gaaggcgcgc atctgacggg cggcaacgtc gtggttcggg
     4321 tggctggcca ggccgggata gtagacgtcc ttgacgccgg ggtcctgctg gagccactcg
     4381 gcgatccgca tcgcgttggc ggaatgctcg cgcatccgaa tccccagcgt cttcagcccg
     4441 cgcagaatca gccagcagtc cagcggaccc ggcacggctc cggccgcgtt ctgatggaac
     4501 ctcaggaagc gcagcacgct ctcgtcgttg ccggccacca gccccagcac gacgtccgag
     4561 tgaccgccga ggtacttcgt cgccgagtgg acgacgagat ccgcgccgtg tgccagcggg
     4621 ttctgcaaat acggcgaggc gaacgtgttg tcgaccagca gcagcggccg ggtcagatcg
     4681 aagccgcgcg taccgctctc ccagctgatc gcctcgccgc tcggcgcacc gtagcgctcg
     4741 cgcagcagct cgctgatccc gccgatgtcg acgatcttca ggtacgggtt ggtcggcgtc
     4801 tccgagatga ccatccgcgt gtcgtcgcgc agtccctcgc gcacctgctc cagatccgac
     4861 agatcgacca tcgagtactc gatgccgtag cgggcgaaga tcttgtcgat cagccggaag
     4921 gtgccgccgt aggcatcgtc cgacatcagg acgtggtcgc ccggcaccaa catgtagagc
     4981 agattctgga tggccgccat ccccgagccg tacgccagcc cccacttgcc gccctccagc
     5041 gaagcgatcg ccgtctgcaa ggcagtccgg gtcgggttat cggttcgcgc gtactcaaac
     5101 cccttgtgct ggccgatccc ggtctgagcg aacgtgctcg tctgatagat cggcacgatc
     5161 accgccccgg tcgtctcctc cggctcctgc ccgtcatgga tcgcgcgtgt ttcgaattgc
     5221 gttttggacc agtcgatgga tgccacagac gtcgttcctc tctctacagg ctgaatcgtc
     5281 gggatttggg attcgggatc agggggtatc gagaggaatc tgtcacttca atactcccga
     5341 atcccgagtc ccgaatcccg atcacccatc gcccttcata ttcgccacaa agtccagcag
     5401 atccgcccgc gtgatgatcc cgaccagcgc gccggtgcgc tggaccagga cggccgaggc
     5461 gccggcggtc gagagcagcg ggaagacgcg ctcgatctct tcggcggcgt cgacgagcgg
     5521 gaacggggcg tccatcacgg tcgagatctt ggcccgcaat gtctccgggc tgcggaagac
     5581 cttgtccagc agcgtccgtt cctggatcga gccggcgatg ttgtggacct cgatcgcgcc
     5641 attgctctgg ccgccggctg tgcggacaac cgggagctgc gagatgttga actcctgcag
     5701 gagcgcgatg gcccgctcga ccgtgtcgtc gacgtggaca gcgacgattt tcggcacgtc
     5761 gtcatcgtgg tagctgctga ccaggtcgcc cgctcgcggc gcctgcgaga agcgcgacag
     5821 gaagccgttc tcgcgcatcc agtcgtcgtt gtagagcttc gagaggtagc cccgcccgga
     5881 gtccggcagc aggacgacga ccagcgcctc cggcccctcg cgcttcgcga cctccagcgc
     5941 cgcccagacc gccagcccgc ccgagccacc gatcaggatc ccctcctcgc gggtgacccg
     6001 tcgcgctgcc gcgaacgagt cgcggtcggt gaccatgatg aactcatcga cggtcgcgcg
     6061 gtcgaacgtg cccggccaga agtcctcgcc gacgccttcg accttgtacg gtcgcgggac
     6121 cggcccggtg tagatcgacc cctccgggtc ggcgccgatc acgcggatgt cggggttctg
     6181 ctccttgagg aacttcgaga tgcccgagat cgtcccgccg gtcccgacgc cggccacgaa
     6241 gtgggtgacc ttgccgtcgg tgccctgcca gatctccggt ccggtcgatt cgtagtgggt
     6301 gcgtgggttc atcgggttga aatactggtt cggctggaag gcgttgggta gctcgcgcgt
     6361 cagccggtcg gcgaccgagt agtacgactc aggcgactcc cgctcgacgt tggtcggcgt
     6421 cgtcacgacc tcggccccgt aggcgcgcag gagcgcgatc ttctcgccgg agaccttgtc
     6481 gggcatcacg aacacgcagc ggtagccctt gatcgccgcc gccatcgcca gcccgacgcc
     6541 ggtgttgccg gaggtcggct cgacgatcgt cccgcccggc ttgatcc
//
LOCUS       DASUGJ010000659         4017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1080208, whole genome shotgun sequence.
ACCESSION   DASUGJ010000659 DASUGJ010000000
VERSION     DASUGJ010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080208"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VFV93_15015"
     CDS             complement(<1..834)
                     /locus_tag="VFV93_15015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5166713.1"
                     /translation="MATETAPRSFDALGLSGETLKALAEIGFEEPTPIQIQAIPPMLA
                     GRDMMAQAQTGTGKTAAFGLPIVERLSGQTRGPEALILAPTRELAVQVAEAIHRFGKY
                     RDTRVVAVYGGQPIERQLRALSRPVDVVVGTPGRLMDHMRRETLKLDNVRFVILDEAD
                     EMLDMGFIEDIETILSALPADRQTALFSATLPKRITDLATQYLTDPVTIRIEPEQVSV
                     PQITQTYYEVVQRAKLEALTRILDLAEPTSAMIFCRTKIETDEVTQALQGRGYAAEAI
                     HG"
     gene            990..2171
                     /locus_tag="VFV93_15020"
     CDS             990..2171
                     /locus_tag="VFV93_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5166714.1"
                     /translation="MPTFEPSENLKRMAAASERPVIGPAPQGMIGLGSGDPDFRTPEH
                     IRQAMIDAINEGYSNYPPGGGDPELLEALAAKMERLSGQPWSPDEVIVTSGGSGALFA
                     SISAWINPGDTVLIPEPTFSQYADVTRLLGGEVVWVRQQEDFHLDLDALRAAAQQHKP
                     KIVVICSPNNPTGVIYSRAELEGLAAIAEEFNMLVLADEAYDHLIHDGHTYVSALEIP
                     ELADRLLYANTFSKTYAMTGMRLGWAAAKGGKINYISRIARMAGGGINWPLQRAGIAA
                     ITGTMEPTEVMRAEYAARRGLIDELLAGVDGVSWNAPQGAFYAYIKYDAPQIKAREMA
                     AIMRERGVALRSGTEYGPSGEGYIRVAFATDRASLTEGMLRVRETLTEAVEGRLKPSA
                     A"
     gene            2296..3207
                     /locus_tag="VFV93_15025"
     CDS             2296..3207
                     /locus_tag="VFV93_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166715.1"
                     /translation="MTVIVRRITRLSVAALALLLAALLAGCVEVQMDSEFNEDGSARH
                     AFEATIDRDGLDQLGSFGGEAVDTFDPEDGREQAEAAGFDYTAIDTDDEVGSRIAKTF
                     DDGEQVGAAFDEMFSAGADEGAEVPVGAVTGTFVKDGDEYRLDLTIDSDLLFSSSGVD
                     DTEDAEDLGFGDLSSFIDITYTATMPGEISETNGTDLGDGKVKWDLPLTGKTEISAVS
                     KAGSNGSGATIAIVGLVALLLVGGLVAGYLFTRRRTPAPATSVGSFTASQTAAPAGSF
                     GTNPPPFEPVDASDERPSHEQDTTKLP"
     gene            complement(3235..>4017)
                     /locus_tag="VFV93_15030"
     CDS             complement(3235..>4017)
                     /locus_tag="VFV93_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX5166716.1"
                     /translation="PWLFGWEPFGLAFDVALHMGTLLAVLIYFRNDLLAMLFGVLRSW
                     RGLLRGQVPDDQMGRLGLYIVAGTIPAVVIGLLVESAIDEYFHQDPVSRTALALSAVT
                     LIAMGVLLGAADRFGRRYGVPESGGRQLRELGLRDAVIVGLAQSFALLPGVSRSGSTI
                     TAGLFAGMSRPVAARFSFLLGVPIILGAGMKESLDVVRDGIPAGERGVFIAGIISAFV
                     VGYLAIAGLMQYLNRHSVDIFVIYRVVVGVGILLLLAAGVRA"
BASE COUNT          723 a         1331 c         1336 g          627 t
ORIGIN      
        1 gccgtggatc gcctcggcgg cgtagccacg tccctggagc gcctgggtga cctcgtcggt
       61 ctcgatcttc gtccggcaga agatcatcgc tgaggttggt tcagccaggt cgaggatgcg
      121 cgtcagcgcc tccagcttgg cacgctggac gacctcgtag taggtctggg taatctgcgg
      181 gacgctgacc tgttccggct cgatccggat cgtgaccgga tcggtgaggt attgggtggc
      241 aaggtcggtg atccgcttcg gcagcgtcgc cgagaagagg gccgtctgcc gatcggccgg
      301 cagggcgctg aggatcgtct cgatatcctc gatgaagccc atgtccagca tctcgtcggc
      361 ctcgtcgagg atcacgaaac ggacgttgtc gagcttgagc gtctcgcggc gcatgtggtc
      421 catcagccgg cccggtgtcc cgacgacgac atcgactggt cgcgagagtg cgcgcagttg
      481 gcgctcgatc ggctggccgc catagacggc gacgacgcgc gtgtcgcggt acttgccgaa
      541 acggtgaatc gcctcggcga cctggacggc tagctcgcgg gtcggcgcga ggatcagcgc
      601 ctccggcccg cgcgtctgac cggagagccg ctcgacgatc ggcagaccga aggcagccgt
      661 cttaccagtc ccggtctgtg cctgggccat catgtcccgc ccggccagca tcggcgggat
      721 cgcctgaatc tggatcggag tcggctcctc gaagccgatc tcggccagcg ccttcagcgt
      781 ctcaccgctc aggcccagtg catcgaacga ccgtggggca gtctctgtcg ccatctaacc
      841 tgcttctctc atcttctgta aggccgcgcg gtaccgacca tgatctgacc cggtacccgc
      901 aagcccgtcg ccaccggaga cgataccgcc ttcgggccga atcgggtagg atggccctca
      961 acgaaccagg agtgtagagg agagacgacg tgccaacgtt tgaaccgtcc gagaacctga
     1021 agcgcatggc agcggcctcg gagcggccgg tgatcgggcc ggccccgcag gggatgatcg
     1081 gcctcggcag cggcgatccg gacttccgga cgccggagca tatccgccag gcgatgatcg
     1141 atgccatcaa cgagggctac tccaactatc cgccgggcgg gggcgacccg gaattgctgg
     1201 aggcgctggc ggccaagatg gaacgcctca gcggccagcc gtggagcccc gatgaggtga
     1261 tcgtcaccag cggcggttcc ggggcgctgt tcgcctcgat ctcggcgtgg atcaatccgg
     1321 gcgacacggt gctgatcccc gagccgacct tctcgcagta cgccgatgtc acccggttgc
     1381 tgggcggtga agtcgtctgg gtcaggcagc aggaggattt ccacctcgat ctggacgcgc
     1441 tacgggcagc cgcccagcag cacaagccga agatcgtcgt catctgcagc ccgaataacc
     1501 cgaccggcgt tatctacagc cgggccgagc tggaggggct ggcagcgatc gccgaagagt
     1561 tcaacatgct cgtcctcgct gacgaggcgt acgaccatct gatccacgac gggcacacgt
     1621 acgtctcggc gcttgagatc cccgagctgg ccgaccggct actgtacgcc aacaccttct
     1681 cgaagaccta cgcgatgacc ggcatgcggc tcggctgggc ggcagccaag ggcggcaaga
     1741 tcaactacat cagccggatc gcccggatgg ccggcggtgg catcaactgg ccgctgcagc
     1801 gggccgggat cgcggccatc accggcacga tggagccgac cgaggtgatg cgggccgagt
     1861 atgccgcccg gcgtggcctg atcgacgagc tgctggccgg tgtcgacggc gtctcgtgga
     1921 atgcgccgca gggcgccttc tacgcctaca tcaagtacga cgcgccgcag atcaaggcgc
     1981 gcgagatggc ggccatcatg cgcgagcggg gtgtcgcgct gcgctccggc accgagtacg
     2041 gcccgagcgg cgagggctac atccgcgtcg ccttcgcgac cgaccgcgcc tcgctgaccg
     2101 agggcatgct gcgcgtccgc gagacgctga ccgaggcggt cgaagggcga ctcaagccca
     2161 gcgccgcctg agacgcgcgt atcagtcaaa acgtcctgtt ctctgccagg aacagggcgt
     2221 tttgtatgcc tgcgaacgtg ccgcatgcta ttgtttcgat acgatgacat tttccgccag
     2281 aggagcattg attcaatgac tgtgatcgtc cgtcgaatca cgcgcctgtc cgttgctgcg
     2341 ctcgcgttgt tgctggctgc gctgctggcc ggttgcgtcg aagtccagat ggatagcgaa
     2401 ttcaacgagg atggcagcgc ccggcacgcc ttcgaggcga cgatcgaccg ggatgggctc
     2461 gaccagctcg gctcgttcgg cggcgaggcg gtcgatacgt tcgaccccga ggacggccgc
     2521 gagcaggccg aagcggccgg cttcgactac acggcgatcg acaccgacga cgaagtcggc
     2581 tcgcggatcg ccaagacgtt tgacgacggc gagcaggtcg gcgctgcctt cgacgagatg
     2641 ttcagtgccg gcgcggacga gggtgctgag gtgccggtcg gcgccgtcac cggcacgttc
     2701 gttaaggacg gcgacgagta tcgcctggac ctgacgatcg actccgacct gctcttctcc
     2761 agcagcggcg tcgacgacac ggaggacgcc gaagacctcg gcttcggcga tctgtcgagc
     2821 ttcatcgaca tcacctacac cgccacgatg ccgggtgaga tcagcgagac caacggcacc
     2881 gacctcggag acggcaaggt caaatgggac ctgccgctga ctggcaagac agagatcagc
     2941 gccgtctcga aggccggcag caatggtagc ggcgcaacga tcgcgattgt cggcctcgtc
     3001 gcgctgctgc tcgtcggcgg tctcgtcgcc ggctacctgt tcacgcgccg ccgaacgccg
     3061 gccccggcca ccagcgtcgg ttctttcacc gcttctcaga ccgctgcacc agccggctcg
     3121 tttggcacga acccgccgcc gttcgagccg gttgacgcat cggacgagcg accatcgcac
     3181 gagcaggaca cgacgaagct gccgtagaac tccccttgtc gtcctgagcc gtagctatgc
     3241 ccggacccct gccgccagca gcagcaaaat gcccacgccg acgacgacgc ggtagatgac
     3301 gaagatatcg accgagtggc ggttgaggta ttgcatcagg ccggcgatcg ccaggtagcc
     3361 gacgacgaag gcgctgatga tgccggcgat gaagacgccg cgctcgccgg ctgggatgcc
     3421 gtcgcgaacg acatccagcg actctttcat gcccgcgccg agaatgatcg ggacaccgag
     3481 cagaaacgag aagcgggccg ccaccggacg gctcattcca gcgaagagac cggccgtgat
     3541 cgtgctgccg gagcgcgaga caccgggtag cagcgcgaag gattgcgcca gcccgacgat
     3601 cacagcgtcg cgcaggccga gctcgcgcag ttgacggcca cccgattccg ggacgccgta
     3661 gcgccggccg aagcggtcgg cggcgccgag cagcacgccc atggcgatca gcgtcacggc
     3721 tgagagcgcc agtgctgtcc gcgaaaccgg gtcctggtgg aagtactcgt caatcgcgct
     3781 ctcgaccagc agcccgatca cgacggccgg gatcgttcca gccacgatgt agagccccag
     3841 ccgccccatc tggtcatccg gcacctgccc gcgcagcaac ccgcgccagc tgcgcaggac
     3901 gccgaacagc atcgccagca ggtcgttgcg gaagtagatc agcaccgcca gcaacgtccc
     3961 catgtgcaac gcgacatcga acgccagccc gaacggctcc caaccaaaca gccacgg
//
LOCUS       DASUGJ010000660         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2053624, whole genome shotgun sequence.
ACCESSION   DASUGJ010000660 DASUGJ010000000
VERSION     DASUGJ010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053624"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFV93_15035"
     CDS             <1..234
                     /locus_tag="VFV93_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5166717.1"
                     /translation="GGAVDTVIDGVTGVHVRQQTVESLIDALTRVDQLRFEPGRLVEH
                     ARSFSRERFEREMIDLIEETMASARDGERRRWN"
     gene            225..911
                     /locus_tag="VFV93_15040"
     CDS             225..911
                     /locus_tag="VFV93_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166718.1"
                     /translation="MELKFYLRVLWRRWPIIVAVPLLVGLLAVGQEVTRDTSYATHAK
                     LRIIAHQLDGDFTDYPADDNFIASEYAIDDMVEAVRGNVFAAAVADRVRATGLEVAHH
                     EVIASISASREHRVLTVTVRSGDPIKVEAIARAATAELEASAFSYIGMATPDSDAVVQ
                     VIDQPGQPGPDNSRARLLLLLEVIAAAGAGVLLAFLIDYLDDTFHDGEAAAATLGLPH
                     LASVPAERST"
     gene            908..1627
                     /locus_tag="VFV93_15045"
     CDS             908..1627
                     /locus_tag="VFV93_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166719.1"
                     /translation="MMRPEEIALALFRRWWIVALAAVAAGLVAYVATSSQPKTYSVST
                     RVMAIAEPPDYWMDLYAKNRLASYKDLIDNWDFVHWALEQAGSDLDPGLVQSKLALGH
                     NPDANIVQIVANDTDPERAATIVNVLADGFVLRNEQENEQLLRRPRPADAPLPGTVTM
                     MKLETPGPPSMASGPRVKVNTLAAGVLGAVAGLMLSFGLLYLDDTLKKTADLERYLEL
                     PVLARIPDRGAAGAGSEGRTG"
     gene            1624..2241
                     /locus_tag="VFV93_15050"
     CDS             1624..2241
                     /locus_tag="VFV93_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166720.1"
                     /translation="MSARQSQAATTRVNAGINEAFRSLRSTVKFAAGEQPIRSVLVVD
                     IDRDTRSSVAEQLATAFAESGDRCLLIDTDARSTADQATGFFDLLMDDAAVDAVTPPP
                     SAENLMRIGPGHAGSPDSLASDQFDATLERLIESHGVAILSSAPLPRYADALAIAPRV
                     DAVILVVTSGGTRRQRAVDARDALERVGARILGVVMVEPKTRRFW"
     gene            2381..>3484
                     /locus_tag="VFV93_15055"
     CDS             2381..>3484
                     /locus_tag="VFV93_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5166721.1"
                     /translation="MLAKVLSCATVGLDGALVEVEVFANSPGGQPGIVVVGLPDAAVQ
                     ESRERVRAAIRSSGGRVPLGRIIVNLAPADLRKEGPAYDLPIALGLLIAGGSLRADLD
                     DAVVIGELSLDGTVRHTTGVLPMVALAARAGLRRAFVPFDDADEAALVDGIAIYPIDT
                     LSGLMKHLNESAPIPPYAGKRDDAFEFEPLATDFSVIRGQEQVKRGLEVAAAGGHNCL
                     MTGPPGTGKTLLARALPSILPPLARDDAIEVSKIYSVAGMLPSGQPLIRERPFRAPHH
                     TVSYAGLVGGGRFPRPGEISLAHRGVLFLDELPEFGQNVLEVLRQPLEDRIVTISRAS
                     GAITYPANFILVAAMNPCPCGYSGDPVRECTCSA"
BASE COUNT          553 a         1139 c         1179 g          613 t
ORIGIN      
        1 ggtggagcag tcgacacggt catcgacggc gtgacagggg tgcacgtccg tcaacaaacc
       61 gtcgagagcc tgatcgacgc gctgacccgc gtcgaccagt tgcggttcga gccggggaga
      121 ttggtcgaac atgcacggtc gttctcacgc gagcgctttg agcgcgagat gatcgacctg
      181 atcgaggaga cgatggcgag cgcacgggac ggagagcggc gtcgatggaa ctgaagttct
      241 acctgcgggt gctctggcgg cgctggccga tcatcgttgc cgtcccgttg ctggttggcc
      301 tgctggcggt cggtcaggag gtcacgcgcg ataccagcta cgccacccac gccaagttgc
      361 gcatcatcgc ccaccagctc gacggcgact tcaccgacta tccggccgac gacaacttca
      421 tcgccagcga gtacgcaatc gacgacatgg tcgaggcggt gcgcggcaac gtcttcgcgg
      481 cggctgtcgc ggatcgtgtg cgcgcgactg gtttggaggt tgcccatcac gaggtgattg
      541 cctccatctc ggcgtcccgt gagcatcgcg tcctgaccgt cacggtccgt tcgggtgatc
      601 cgatcaaggt agaagcgatc gcgagggcag cgacggcgga actcgaagcg tcggccttca
      661 gctacattgg gatggcgaca cccgattccg acgcggttgt ccaggtgatc gaccagcccg
      721 gccagccggg acccgacaac tcccgcgcca ggctgttgct gttgcttgag gtgatcgcgg
      781 ccgccggagc aggcgttctt ctggcattcc tgatcgacta cctcgacgac acctttcacg
      841 acggcgaggc ggctgccgcg acgctcggcc tgccgcacct cgccagcgtt ccggcggagc
      901 gctcgacgtg atgcgtccgg aggagatcgc gctcgcgctg tttcggcgct ggtggatcgt
      961 cgcgctggca gcggtcgctg ccgggctggt tgcctatgtc gccaccagca gccagccgaa
     1021 gacatactcc gtcagcaccc gcgtgatggc aattgccgaa ccgcccgact actggatgga
     1081 cctctacgcc aagaaccggc tggcgtcgta caaggatctg atcgacaact gggacttcgt
     1141 ccactgggcg cttgagcagg ccgggtccga tctcgatccg gggctcgtcc agtccaagct
     1201 ggcgctcggc cacaatccgg acgcgaacat cgttcagatc gtcgccaatg acaccgatcc
     1261 ggagcgggcg gccacgatcg tcaacgtcct cgccgacggc ttcgtcctgc gcaacgagca
     1321 ggagaacgag cagctgctcc ggcggccccg gccagcggac gcaccgctgc ccggcactgt
     1381 cacgatgatg aagcttgaaa cgcccggccc gccatcgatg gccagcggtc cgcgtgtcaa
     1441 ggtcaacacg ctggcggccg gtgtgttggg cgctgtcgcc ggactgatgc tatcgttcgg
     1501 actgctctat ctggatgaca cgttgaagaa gacggccgat ctcgagcggt atctcgaatt
     1561 gccggtgttg gcgcggatcc ccgatcgcgg cgcggcgggc gcgggaagtg aaggacgaac
     1621 aggatgagcg cgcgccagtc acaggcggca acgactcggg tgaacgctgg catcaacgaa
     1681 gcgtttcgct cgcttcgctc gacggtgaag ttcgcggccg gggagcaacc gattcgctcc
     1741 gtcctcgtcg tcgatatcga ccgcgatacg cgcagcagcg ttgccgagca gcttgcgacc
     1801 gcgtttgccg agtccggcga ccgctgcctg ctgatcgaca ccgacgcacg cagtactgcc
     1861 gatcaagcga ctggcttctt cgacctgctc atggatgatg cggcagtcga tgccgttact
     1921 ccgccgccga gcgccgagaa cctcatgcga atcggaccag ggcacgccgg gtcgccagat
     1981 tcgctggcga gcgatcagtt cgatgcgacg ctcgagcggc tgatcgaatc gcacggcgtc
     2041 gcgatcctgt cgtcagcgcc gctgccgcgc tacgccgatg cgctggcgat cgcgccgcgc
     2101 gtcgatgccg tcattctggt tgtgacatcc ggcggaacgc gccggcagcg cgcggtggac
     2161 gcccgcgatg cgctggagcg ggttggggcg cggattctcg gcgtcgtgat ggtcgaaccg
     2221 aagacgcgcc gcttctggta gcccggcgct catttccgca acaggtcgct tcttcgccaa
     2281 tcgtgtgtcg tgctacactg cgccggtccg aacacggtcc gggtccgtcc ctgctgccgg
     2341 ctggcgcggg tggatacctg cgtgaggtgg aggaggcgga gtgctggcga aggtcctcag
     2401 ttgcgcgaca gttgggctcg atggcgcgct ggtcgaggtc gaggtcttcg ccaactcgcc
     2461 gggcggccaa ccggggattg tcgtcgtcgg cttaccggac gccgccgtcc aggaatcgcg
     2521 cgagcgcgtc cgggccgcca ttcgcagtag tggcggtcgc gttccgctgg ggcggatcat
     2581 cgtcaacctg gctccggccg acctgcgcaa ggaggggccc gcctacgatc taccgattgc
     2641 gctgggtctg ctgatcgccg gtggctcgtt gcgagccgac ctcgacgatg ccgtcgtcat
     2701 tggtgagctg tcgctcgacg gtaccgtccg ccacacgacc ggcgtgctgc cgatggtcgc
     2761 gctggccgca cgggcggggt tacgacgcgc attcgtcccg ttcgacgacg ccgatgaggc
     2821 ggcgttggtc gacggcatcg ccatttaccc gatcgacacg ctctcggggc tgatgaagca
     2881 tctcaacgag agtgcgccaa tcccgccgta tgccggcaag cgggacgatg cgttcgagtt
     2941 cgagccgctg gcgaccgact tctcggtcat tcgcggccag gagcaagtca agcgcgggct
     3001 ggaggtcgcg gcggcagggg gccacaactg tctcatgaca ggcccacccg gtaccggcaa
     3061 gacgttgctg gcccgcgcac tgccgtcgat cctgccgccg cttgcgcgcg acgacgcgat
     3121 cgaggtctcg aagatctact cggtcgccgg catgctcccc tccggccagc cgctgatccg
     3181 cgagcggccc ttccgcgcac cgcatcacac cgtctcatac gccgggctgg tcggtggtgg
     3241 gcgttttccc cgtccgggag agatcagcct cgcccatcga ggcgtgctct tcctcgatga
     3301 actgcccgag ttcggccaga acgtgctgga ggtgctgcgc cagccgctgg aagaccggat
     3361 cgtaacgatc tcccgcgcca gcggggcgat cacctatccg gccaatttca ttctggtagc
     3421 ggccatgaac ccgtgtccat gcgggtattc aggcgacccg gtccgcgagt gtacctgctc
     3481 ggcg
//
LOCUS       DASUGJ010000661         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1913550, whole genome shotgun sequence.
ACCESSION   DASUGJ010000661 DASUGJ010000000
VERSION     DASUGJ010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913550"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VFV93_15060"
     CDS             complement(<1..771)
                     /locus_tag="VFV93_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEX5166722.1"
                     /translation="MVDTAAAPQPAQANQRKLTLGVPREIADNERRVALIPDTVKRLV
                     AQGLQVLVETDAGTNAAFVDSAYSDAGATIVPDARSVYSGADMVIKIQRPSDEELDLL
                     RSGQILIAMLSPLVNTELAVDLARRGVTSFSMDAIPRITRAQSMDVLSSQATVAGYKA
                     ALLAAEHATKFFPMLTTAAGTIPPAKVLVLGAGVAGLQAIATARRLGAVVSAFDTRPV
                     VKEQVESLGASFLEIEVSGGETSGGYATQLSEDQHRLEQ"
     gene            1101..2669
                     /locus_tag="VFV93_15065"
     CDS             1101..2669
                     /locus_tag="VFV93_15065"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5166723.1"
                     /translation="MIARSIRAVLIVAVLLPLFGTSGDAAGPWNRSFARTWARTDRPV
                     ADLLADRTWMWGPEAFTPGLLEPYAGAPNGQRLVQYYDKSRMEITTDPAITPASAWYV
                     SNGLLAKELITGLMQLGDEVFEPHLSAEINVAGDPDDPTGPTYWTFASVLNVAPPPDG
                     APIVQRITRAGLLPDDPGLAARGVTAAHRVQVAGIDHQIASPFWAFMNGSALVYQDAA
                     FRTAPLFENPFYATGFPITEAYWSRIKLKGVYSDVLIQCFERRCLTYTPDNPEGWKVE
                     AGNVGRHYYEWRYAASQPAPAPPDDAPVIVLDAGHDSTTGGANGIEYQDTLRTALATR
                     DALEAAGYTVFLTRPGDDTVLYGDPALMPANAGSMPLDYNQGYAHTTRALQFEPDLYI
                     SLHYNGSSSPDAGGMTIYYCDYGGTQNAGLAVIIRDELLAALRSQGYEPPYANATEDG
                     AIGKSYGHLATLGNVYSAPFVFEGDRLAGVPSVLVEPLFETNPTERALIQDDATIAAF
                     AQAYVRAANRYFGR"
     gene            complement(2776..>3298)
                     /locus_tag="VFV93_15070"
     CDS             complement(2776..>3298)
                     /locus_tag="VFV93_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166724.1"
                     /translation="AVAALLFVFLISADVIGNIDTGTGDDDDSAPIAMDAPAPAGAEL
                     GATATTSAASVMGAPEDEAADTERSSNAEATPASGGAEAATGPTGESDDASTSENAET
                     MLATETPVPDQFELAQRDDTTVAGYGSANDITSDDSAESNTLYLLELALAITIAWLIV
                     AMIALPRLRRPQG"
BASE COUNT          504 a         1194 c         1057 g          543 t
ORIGIN      
        1 ctgctcgagc cggtgctggt cttcagagag ctgggtcgcg taccccccgg aggtctcgcc
       61 tcccgacacc tcgatctcga ggaaggacgc accgaggctc tcgacctgct ccttgacgac
      121 aggccgcgta tcgaacgccg acacgacagc gccgagccgc cgcgctgtcg cgatcgcctg
      181 gagaccggcc acaccggccc cgaggacgag caccttggca gggggtatcg tccccgcggc
      241 cgtcgtcagc atcggaaaga acttcgtcgc gtgctccgcc gcgagcagcg cggccttgta
      301 gcccgccacc gtcgcctgcg aggagaggac gtccatcgac tgcgcgcggg tgatgcgcgg
      361 aatggcatcc atgctgaagc tggtcacgcc gcgccgcgcc agatcgacgg ccagctcggt
      421 gttgaccagt ggcgagagca tcgcgatcag gatctggccg ctgcgcagca gatcgagctc
      481 ttcgtcggac ggccgctgga tcttgatgac catatcggcg ccgctgtaga cgctcctggc
      541 gtccggcacg atcgtcgcgc cggcgtcgct gtaggcgcta tcgacgaagg ccgcgttcgt
      601 gccggcgtcc gtctcgacca gcacctgcaa gccctgcgcg acgagacgct tgacggtgtc
      661 gggaatcagc gcgacgcgcc gctcattgtc cgcgatctcg cgcggcacgc cgagcgtcag
      721 cttgcgctgg ttcgcctgcg ccggttgggg cgctgctgcg gtgtcgacca tgctgggtct
      781 gcctccccgg tgtttacgtg accacggcca gccggcgcgc atgccaggct gcccgaaccc
      841 cctgccacaa caggccggat agaccagtac gccgccggcc tctcgaccgg ggcgacacca
      901 tccaactccg cctgcgtgcg agcagccgcc cgggaccagc cattggctgc tccctcggtc
      961 atcatcgtct gtcaggatgg ctgactggca gactgccctc agattgtgaa gtttacgaca
     1021 atttctgtcg cgggacgagt cacgatagaa tcgatcccgt cgcgtcatgc cgaagcgccg
     1081 tcctcgcgga aggcccgccc atgatcgccc gttccatccg cgccgtcctg attgtcgccg
     1141 tgctgctgcc gctgttcggc acctccggtg acgcggccgg gccgtggaat cggtccttcg
     1201 cccggacctg ggcgcgaacc gaccgtcccg tcgccgatct gttagccgac cggacctgga
     1261 tgtgggggcc ggaagccttc actcccggcc tgctggagcc gtacgccggg gcgcccaacg
     1321 gccagcggct cgtccagtat tacgacaagt cgcggatgga gatcacgacc gatcccgcca
     1381 tcactcccgc cagcgcctgg tacgtctcca acgggctgct ggcgaaggag ctcatcaccg
     1441 gcctgatgca actgggcgac gaggtcttcg agccgcacct gtcggccgag atcaacgtcg
     1501 ccggcgaccc cgatgacccg accgggccga cctactggac cttcgccagc gtcctcaacg
     1561 tcgccccgcc acccgacggc gcgccgatcg tccagcgcat cacccgcgcg ggattgctgc
     1621 cggacgaccc cggcctggcc gcccgtggtg tcacggcggc tcaccgagtc caggtcgccg
     1681 gtatcgacca tcagatcgcc tcgccgttct gggcgttcat gaacggcagc gcgctggtct
     1741 atcaggacgc cgccttcagg accgcgccgc tcttcgagaa cccgttctac gcgaccggct
     1801 tcccgatcac cgaggcctac tggtcgcgga tcaagctcaa aggtgtctac agcgacgtcc
     1861 tgatccagtg cttcgagcgt cgctgcctga cctacacgcc cgacaacccg gagggctgga
     1921 aggtcgaagc gggcaatgtc ggccgccact actacgagtg gcgctacgcc gccagccagc
     1981 ccgccccggc accgcccgac gacgcaccgg tgatcgtcct cgacgccggc cacgattcca
     2041 ccaccggcgg agcgaacggc attgagtacc aggacacgct gcggaccgct ctggcgaccc
     2101 gcgacgcgct cgaagcggcc ggctacaccg tcttcctgac tcgccccggc gatgacacgg
     2161 tcctctacgg cgatccggcg ctgatgccgg ccaatgccgg tagcatgccg ctcgactaca
     2221 accagggcta cgcgcacacc acccgcgcgc tccaattcga gccggacctc tacatctcgc
     2281 tccactacaa cggctcttca agccccgacg ccggcgggat gaccatctac tactgcgact
     2341 acggcggcac ccagaacgcc ggcctggccg tcatcatccg cgacgagctg ctggccgcac
     2401 tgcgctcgca gggctacgag ccaccctatg ccaacgcgac cgaggacggc gcgatcggca
     2461 agtcgtacgg ccacctcgcg acgctcggca acgtctacag cgccccgttt gtcttcgagg
     2521 gcgaccgcct ggccggtgtc ccatccgtcc tcgtcgaacc gctcttcgaa actaatccga
     2581 ccgaacgcgc cctcatccag gacgacgcga cgatcgccgc cttcgcccag gcgtacgtgc
     2641 gcgctgcgaa ccggtatttt gggcggtgat gtgggtcgaa acccctcccc cacgggggga
     2701 gggggctctt gattcgtgcg gcctgaggcc ccctctctta ttaggggagg gggttcgact
     2761 cacaacgacg acccgctacc cctgcggtcg gcgcagccgc ggcagggcga tcatcgcgac
     2821 gatcagccag gcgatcgtga tcgcgagcgc aagttccaac aggtagaggg tgttgctctc
     2881 ggcgctgtcg tcggaggtga tgtcgttggc gctgccgtag ccggcgacgg ttgtgtcgtc
     2941 ccgttgggcc agctcgaact ggtccgggac cggcgtctcg gtcgccagca tcgtctcggc
     3001 gttctcggat gtcgaggcgt cgtcgctctc cccggtcggg ccggttgcgg cttctgcgcc
     3061 gccggatgca ggcgttgcct cggcattgga gctgcgttcg gtgtcggcgg cctcatcttc
     3121 gggcgcgccc atcaccgacg cagcggatgt cgtcgccgtt gcgccgagtt cggcgccggc
     3181 tggggctggc gcatccatcg cgatcggggc ggaatcgtca tcgtcgccgg tgccggtgtc
     3241 gatgttgccg atcacgtcgg cgctgatcag gaagacgaac agcagcgctg ccacggcc
//
LOCUS       DASUGJ010000662         3983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_663339, whole genome shotgun sequence.
ACCESSION   DASUGJ010000662 DASUGJ010000000
VERSION     DASUGJ010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663339"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..777
                     /locus_tag="VFV93_15075"
     CDS             394..777
                     /locus_tag="VFV93_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5166725.1"
                     /translation="MNLTLWIVAGLMATVFLFAGSTKLFIPREKLAKAPGGDWVMDFS
                     SVFVKGLGAIEILGAIGLILPAALDIAPVLVPLAAVGLATIMVGAAVVTYRRHEVKHV
                     LVNLTYLALIVFVAVGRVAFESFSG"
     gene            874..1815
                     /locus_tag="VFV93_15080"
     CDS             874..1815
                     /locus_tag="VFV93_15080"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5166726.1"
                     /translation="MRVFVAGATGAIGQQLVPRLVAAGHEVHGMTRSESKQAMLLDMG
                     AIPVVADALNADQVAEAVGRAMPDVIVHQLTAFGSLGSANPRHFDRDLAPTNRLRTEG
                     TDHLLSAGHAVGVQRFVAQGFGPISYARTGGPVKTEDDPLDPAPVPVMRETLAAIRHL
                     EQAVTGARWTEGIVLRYGGFYGPGTAIAPGGEMYEMIRKRRFPLVGDGGGVWSFVHIA
                     DAAEATLAAVEHGTRGIYNVVDDDPATVAEWLPELARTIGAGKPRHVPTFLVRLLAGE
                     AGVVMMTDLRGASNAKAKRELGWRPAHPSWREGFAAL"
     gene            1812..2729
                     /locus_tag="VFV93_15085"
     CDS             1812..2729
                     /locus_tag="VFV93_15085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX5166727.1"
                     /translation="MTDRERLLDELRPASFAVAYRMLGSVSEAEDVVQEALLRVHQAL
                     DAGEQIASPRAFIVTVTTRLAINELRSARVRRETYVGEWLPEPIITNSHDDPAWHVET
                     AESLSLAMLALMERLSPEQRAVLLLHDVFDYGYPEVTEIVGKSEDNVRQLATRARRRV
                     RQRRRRYTTTREERDELARRFFAAAEGGDLAGLEALLADDVVLTGDGGGKAPAMARSL
                     RGCTRVASVLINGFRLFTRLPGVTLRLSEVNGGPGALLYDGQQRLLGVMALDITGGQI
                     TSVNSVVNPDKLSHLGPVGDVKALLRSAT"
     gene            2862..>3983
                     /locus_tag="VFV93_15090"
     CDS             2862..>3983
                     /locus_tag="VFV93_15090"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5166728.1"
                     /translation="MIVTDRQQEAFRSETSEAARLYRRAKQVMPGGNSRTTVYQSPYP
                     PYAARGVGAVVVDADGQERYDFLNNYTSLIHGHADPDINAAVIEQLQYGTAFAMPTEH
                     EVVLAELLVERIPSLERMRFTNSGSEAVMMAIKAARAYTGRPKIVKFEGCYHGSYDFA
                     EVSLSSNPSNWGEGDPVSIAYSYGTPQSVLDEVIVLRYNDTEMAERRITEHAHELAAV
                     LIDPMPLRAGLIPAEPEFLQRLRDLCTAHGIVLIFDEVISLRTAYGGAQETLGVTPDL
                     TTMAKIIGGGFAVGAFGGSAEVMSVFDPSQGYPRAPHGGTFNANPITMVAGLVAMQKL
                     TREEFARLDQLGAMLREGLAEAMDGARVKGQVTGMGSLAQ"
BASE COUNT          632 a         1289 c         1382 g          680 t
ORIGIN      
        1 ggtcgcgccg agcttcaccg tgcacacctg ccaaccggca ctgtgactct tgcgcacgtt
       61 gctcgttgcg cgatcatgga cctccgcgtc gagccgcgcc gtgacgactg gcagcgcatc
      121 ctcgatgacg caaactagct cacgccgctg gcggtagctt cagccgaatt tggacgatct
      181 gctgacggcg ccggtccgag cgactcgacg cgagcgcctt atctccaaca cagcacggtc
      241 gaagccaatc gagtgagacc gtgcctcgcc tcagcacgac tcccttgtct tcgcgcgccg
      301 caattccgtg ccggtgtgcc cggaggtgtc acagcctggg gcgccatctt gtcttatggg
      361 gtgagagtcg ttctcgtatc gaaggagctc atgatgaatc tcactctgtg gatcgtggcc
      421 ggcctgatgg cgacggtctt tctgtttgcc ggctcgacca agctgttcat cccgcgcgag
      481 aagctggcca aggcgcccgg tggcgactgg gtcatggact tcagttccgt cttcgtcaag
      541 ggactcggtg ccatcgagat cctcggcgct atcggcctga tcttgccggc cgcgctcgac
      601 atcgcgccgg tgctggtgcc gctggcagcc gtcggtctgg cgacgatcat ggtcggcgcg
      661 gcggtcgtga cctaccgccg tcacgaggtc aagcacgtgc tggtgaacct gacctatctc
      721 gctctgatcg tcttcgtcgc agttgggcgc gtcgccttcg agtccttcag cggctgaccg
      781 ggccacggcg tccgactgcc tatccgatag ccgagcgccg gtagcgccgg acgctcggct
      841 cccaagaggt acacgttacg aaaggaactg atgatgcgag tgtttgtagc aggtgcgact
      901 ggcgcgattg gacagcagtt ggtgccgcgt ctggttgccg ccggtcatga ggtgcatggc
      961 atgacgcgca gcgagtcgaa gcaggcgatg ctcctcgaca tgggcgcgat accggtcgtt
     1021 gcggatgcgc tcaatgctga ccaggtcgcc gaggccgtcg gccgggcgat gccggacgtg
     1081 atcgtccacc agttgaccgc gttcgggtcg ctggggtcgg cgaacccgcg ccactttgac
     1141 cgcgatctcg cgccgaccaa ccggctgcgg accgagggga cggaccacct gctctcggcc
     1201 gggcacgcag tcggtgtgca gcgcttcgtg gcgcagggtt ttggcccgat ctcctacgcg
     1261 cgtaccggtg ggccggtcaa gaccgaggat gacccactcg atcccgcgcc tgtcccggtc
     1321 atgcgcgaga cgctggcggc gatccgccat ctggagcaag cggtcaccgg cgcgcggtgg
     1381 acggaaggga tcgtgctgcg ttacggtggc ttctacgggc cgggaaccgc gatagcgccg
     1441 ggcggggaga tgtacgagat gatccgcaag cgaaggttcc cgttggtcgg tgacggtggc
     1501 ggcgtctggt cgttcgtgca catcgccgac gcggccgagg ccacgctggc agcggtcgag
     1561 catggcacac gcggtatcta caacgtcgtc gatgacgacc ccgcgaccgt ggccgagtgg
     1621 ctgccggaac tggcccggac gatcggcgcc ggaaagccgc gccacgtacc gacgttcctc
     1681 gtccgattgc tggccggcga ggccggcgtc gtgatgatga ccgatctgcg cggtgcgtca
     1741 aatgcgaagg cgaagcgcga gttggggtgg cgtccggcac acccgagctg gcgggaagga
     1801 ttcgcagcgc tatgactgat cgtgaacgtt tgctcgatga gttgcgaccg gcctcgttcg
     1861 cggtcgccta ccggatgctc ggcagcgtct ccgaggccga agatgtggtc caggaggcgt
     1921 tgctgcgggt gcatcaggcg ctcgatgcgg gcgagcagat cgcctcgccg cgggcgttca
     1981 tcgtgacggt gacgacccgg ctggcgatca acgagttgcg ctcggcccgc gttcgccgcg
     2041 agacctatgt cggcgagtgg ctgccggagc cgatcatcac caacagtcac gacgatccgg
     2101 cctggcacgt tgagacggcc gagtccctgt cgctggcgat gctggcgctg atggagcgcc
     2161 tctcgcccga gcagcgcgcc gtcctgctgc tgcacgatgt gttcgactac ggctatccgg
     2221 aggtgaccga gatcgtcggc aagagtgagg acaacgtgcg ccagctggct acccgcgcca
     2281 ggcggcgcgt gcggcagcgc cgccgtcgct acaccacgac gcgcgaggag cgcgacgaac
     2341 tggcccggcg gttcttcgcg gccgccgaag gcggcgatct ggccgggctg gaggcgctgc
     2401 tggcggacga cgtcgtgctg accggggatg gcggcggcaa ggctccggcg atggcccgct
     2461 cgctgcgcgg ctgtacccgg gtcgcgtcgg tgctgatcaa cgggttccgg ctgttcaccc
     2521 gacttccggg cgtgacattg cgcctcagcg aggtcaacgg tggcccggga gcgctcctgt
     2581 acgatgggca gcagaggctg ctcggtgtga tggcgctcga catcaccggc gggcaaatca
     2641 cgagcgtcaa ctcggtcgtc aaccccgaca agctgtcgca cctcgggccg gtcggcgatg
     2701 tcaaagcgct gctgcgctcc gcgacgtgat gtcctggtag cccccgattg ccccgtggga
     2761 gttgtgtcgc gccggtgatg cctctactct atgagggacg acaccgactg acgtgagcag
     2821 atgctggcga acgcgggagt gagcgaaagg actggcgcgg gatgatcgtc actgacaggc
     2881 agcaggaagc gtttcggtcc gagacatctg aggcagcgcg gctgtatcga cgggcaaagc
     2941 aggtcatgcc gggcggcaac tcgcgcacga ccgtctacca gtcgccctac ccgccttatg
     3001 ccgcccgtgg ggttggcgcc gtcgtcgtcg atgcggacgg ccaggagcgc tacgatttcc
     3061 tcaacaacta cacctcactg atccacggcc acgccgaccc cgacatcaac gccgccgtca
     3121 tcgagcagct gcagtacggc accgccttcg cgatgccgac cgagcacgaa gtcgtgctgg
     3181 cggagctgct ggtcgagcgg attccctcgc tggagcggat gcgcttcacc aactccggct
     3241 cggaagcggt catgatggcg atcaaggccg cccgcgccta caccggccgg ccgaagatcg
     3301 tcaagttcga gggctgctac cacggctcgt acgacttcgc cgaagtcagc ctcagctcga
     3361 atcccagcaa ctggggcgag ggcgacccgg tctcgatcgc ctactcctac ggcacaccgc
     3421 agtcggtcct cgacgaggtg atcgtcctgc gctacaacga caccgagatg gccgaacggc
     3481 gcatcacgga gcacgcgcac gaactggcgg ccgtcctgat cgacccgatg ccgctgcggg
     3541 ccggcctgat cccggccgag ccggagttcc tgcaacggtt gcgcgatctc tgcacggcgc
     3601 acgggatcgt cctgatcttc gacgaggtca tctcgctacg gacggcctac ggcggcgcac
     3661 aggagacact cggcgtgacg ccggacctga cgacaatggc caagatcatc ggcggcggct
     3721 tcgcggtcgg cgccttcggc ggctcggccg aggtgatgag cgtctttgat ccgtcgcagg
     3781 gctacccgcg cgctccgcac ggcggcacct ttaacgccaa cccgatcacg atggtcgccg
     3841 gcctggtcgc gatgcagaag ctgacgcgcg aggagttcgc gcggctcgac cagcttggtg
     3901 cgatgctgcg cgagggactg gccgaggcga tggacggtgc ccgcgtcaag ggtcaggtga
     3961 ccgggatggg ctcgctggcc cag
//
LOCUS       DASUGJ010000663         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_942702, whole genome shotgun sequence.
ACCESSION   DASUGJ010000663 DASUGJ010000000
VERSION     DASUGJ010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942702"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..2158)
                     /locus_tag="VFV93_15095"
     CDS             complement(194..2158)
                     /locus_tag="VFV93_15095"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX5166729.1"
                     /translation="MSAEPIAHLSAQPVVWRPGPAQIERSRALAFARQHGLDGWDALV
                     ERAAEDPNWFWGAVSDELGLVWTRQYDQVLDLRDGPEWPRWYSGGRMNYVTSAVDRWL
                     PERAGETAVIWEGDDGATGSLTYAELAEAVNRMANGLRSLGIGKGDRVGIYLPMLLET
                     AVATLACGKIGAIFIPIFSGYGPEAAATRLNDGGAKLLITADGFYRRGRIIPMKETAD
                     AAVALAPSVEQVLVVHRTGADVPWDDARDRWWDNVAQASSGECEPEDTAADDPYMLIY
                     TSGTTGKPKGTVHVHAGFPVKAAQDMAFCFDVQRDDTLFWLTDLGWMMGPWAIAGALI
                     AGSNVLLYEGTPDFPAPDRLWHVVERHGATVLGISPTVVRALMGQGDDWVERHELASL
                     RVLGSTGEPWNPGPWQWTFEKVGKGRCPIVNYSGGTEIGGGIIAAVPVKPQKPCAFSG
                     PVPGVAADVVDDNGRPVRGVVGELVIRGPWVGMTSGFWGDRERYIEAYWSRIPGLWVH
                     GDWAMIDDDGFWYILGRSDDTLKVAGKRVGPAEIESAVVAHPAVQEAAAIGIPHEVKG
                     EAIVVFAILKPGQTGDSATEDAIRDTVARQLGRPLRPDRVVFVSDLPKTRNAKIMRRV
                     IRAKYLGRTDFGDLSSLENPAAVEEIKAGG"
BASE COUNT          382 a          863 c          752 g          394 t
ORIGIN      
        1 ccctgttatc caccgcccag agggcacctg gcagcgaagg atcgctcaac gcttgaagga
       61 tggtcgcgcc aatcggaatg atggcgctgg ctcgtccgat ccccatttca tcaatgacca
      121 gcgatccttc gctgcggatc agggtgacag gggtgcgggg ctcggtcctc gtttacgccg
      181 tccggctggc tagtcaaccc ccagccttga tctcctccac agccgcaggg ttctccagcg
      241 acgagagatc gccgaagtcg gttcggccca gatacttggc gcggatgacg cggcgcatga
      301 tcttggcgtt gcgcgtcttc ggcagatcgc tgacgaagac gacccgatcc ggccgtagcg
      361 gccggccgag ttggcgagca accgtgtccc ggatcgcgtc ctcagtcgcg ctgtcccccg
      421 tttgccccgg ctttaggatc gcgaagacga cgatcgcctc gcccttgacc tcgtgcggaa
      481 tgccgatcgc ggccgcttcc tgaacggccg gatgggcgac gacggccgac tcgatctcgg
      541 ccggcccgac ccgcttgccg gccaccttga gcgtgtcatc cgaccggccg aggatgtacc
      601 agaagccgtc gtcgtcgatc atcgcccagt cgccgtgcac ccaaaggccc ggtatccgcg
      661 accagtacgc ctcaatgtac cgctcgcggt cgccccagaa gccgctcgtc atcccgaccc
      721 atggaccgcg aatcaccagc tcgccgacga cgccgcgtac cggccggccg ttgtcatcga
      781 cgacatcagc ggcgacgccc ggcaccggcc cgctgaaggc gcatggcttc tgcggcttga
      841 ccggcacggc cgcgatgatc cccccgccga tctcggtccc gcctgagtag ttgacaatcg
      901 ggcagcggcc tttgccgacc ttctcgaacg tccactgcca cggcccggga ttccacggct
      961 caccagtcga gccaagcacc cgcagcgagg ctagctcgtg ccgctcgacc cagtcgtcgc
     1021 cctgccccat caacgcccgg acaaccgttg gcgagatacc gaggacggtc gcgccatgcc
     1081 gttcgacgac gtgccagagc cggtccggag ctgggaagtc gggcgttccc tcgtagagca
     1141 ggacgttact acccgcgatc agcgcaccgg cgatcgccca gggccccatc atccagccca
     1201 ggtcggtcag ccagaagagc gtgtcgtccc gctggacatc gaagcagaac gccatatcct
     1261 gggcggcttt gactgggaac ccggcgtgga cgtggaccgt ccccttcggc ttgccggtcg
     1321 tgccggaggt gtagatcagc atgtacggat cgtcagcggc cgtatcctcc ggctcgcact
     1381 cgccgctcga cgcctgggcg acgttgtccc accagcggtc acgcgcgtcg tcccatggca
     1441 cgtccgcgcc ggtccggtgg acgaccagca cctgctcgac cgacggggcc agcgcgacgg
     1501 cggcgtcggc cgtctccttc atcgggatga ttcgcccgcg ccggtagaag ccgtcggcgg
     1561 tgatcagcag cttcgcgccg ccgtcgttca accgggtcgc cgccgcctcc ggcccgtaac
     1621 cggagaagat cgggatgaag atcgcgccga tcttgccgca ggccagtgtc gcaaccgccg
     1681 tctccagcag catcggcagg tagatgccga cccggtcgcc cttgccgatc cccagcgagc
     1741 gcagcccgtt ggccatccgg ttgactgcct cggccagttc cgcgtaggtc aggctgccgg
     1801 tcgcgccatc gtcgccctcc cagatgacgg ccgtctcccc ggcccgctcc ggcagccagc
     1861 ggtcgaccgc gctggtgacg tagttcatcc gcccgccgct gtaccagcgc ggccactccg
     1921 gcccgtcgcg caggtcgagc acctggtcat actggcgtgt ccagaccagc ccaagctcgt
     1981 cggagaccgc accccagaac cagttggggt cctccgccgc ccgctcgacc agcgcgtccc
     2041 agccatccag cccatgctgc cgtgcgaatg ccagcgcccg gctgcgttcg atctgagccg
     2101 gccccggccg ccagacgacc ggctgggcag acaaatgggc aatcggttcg gcgctcatgg
     2161 cattgctccc tgaatggtca cggcacgtat catctcgttc tggattgtgg tctttgcggg
     2221 acggaacggt caacacccaa ccggtgttgg ccagcacggc aacgatggcg ttagactggc
     2281 aggtgtggaa ccagcagtgt gggctatccc gccacctttc gcgccggtgg cgggcgacac
     2341 ggctgcgtga cgaatctggt cagagcgaac aagggaacct ggtatgagtg t
//
LOCUS       DASUGJ010000664         2849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_663559, whole genome shotgun sequence.
ACCESSION   DASUGJ010000664 DASUGJ010000000
VERSION     DASUGJ010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663559"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFV93_15100"
     CDS             <1..592
                     /locus_tag="VFV93_15100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5166730.1"
                     /translation="VAGTVLDVTERKQLETRLSHQAYHDPLTGLSNRAHFLERLEEHQ
                     RQDTVAVLFIDLDNFKEVNDRLGHNAGDRVLCIIAERLRLCLRDGDLAARLGGDEFTV
                     LLQGLTAPHSVDSVARRILQVLSEPIAIDGESVLITPSIGVAITTPDLDDTSEGLLRA
                     ADTAMYRAKRDGKARYAVFSRDGSDDDDTLQLVKAS"
     gene            complement(1061..>2849)
                     /locus_tag="VFV93_15105"
     CDS             complement(1061..>2849)
                     /locus_tag="VFV93_15105"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX5166731.1"
                     /translation="GEPVAVDALWFASDGQSVQGGTSRVVVSVNSKDDQDLRVGFFES
                     EVGGSGPQWRAAGWMAVITASLLIGNDPSHYEFSFDVAGRIDGPSAGALMTIAVLAGY
                     LGDEIDPTVTMTGTINPDGTIGPVGGIPHKIEGAAAAGKTTVLVPGGQRYDYDYALGQ
                     SVDLVQVGQQNGVEVKLVSDIYDAYRELTGSELPAPANTGQAAFPSSAFDKYRAGATN
                     WYATYQSEVNDFYALPADVQEYRLETIQAADYYAGMADQYLGEGQIAAAYENAFTAAA
                     LARVAREAAELDNIYYQQGIGPLVDRLNSSSSAQTRMSAVSQRLEAETPRTAADSIAI
                     MDAFSNLSVAQGLIFQADAALNDYANNPDATEDDALTAIYAANYNYVYADMYLDLAED
                     GLGIGLGFGNSPAPSLEVLTAISETLRRGAEANITYFESLIIDPWAQQYGIHPDLAKY
                     YFQQSDNSYLTAVAAVAGASSLAGTMLKPEAQASVNLGSALTAYSQSASLIAKYYSLS
                     AQVDESGSVVGYDRQSSLVDMLDLADQRAQQWLGAVAGEDPVQSLYYYDNARLLRSGT
                     PDDQLSALNYFWQSAILSETLAVFATPAE"
BASE COUNT          481 a          869 c         1010 g          489 t
ORIGIN      
        1 ggtcgccggg accgtcctcg acgtgaccga gcgcaagcag cttgagacac ggctgtcgca
       61 tcaggcctat catgacccgc tgaccggcct ctcgaaccgt gcccacttcc tggagcggct
      121 ggaggaacac cagcggcagg acacggtcgc cgtcctgttc atcgacctcg acaacttcaa
      181 ggaagtcaac gatcggctgg gacacaacgc cggcgatcgg gtgctctgca tcattgccga
      241 gcggctccgc ttgtgcctgc gcgacggtga cctggcggcg cggctgggtg gcgacgagtt
      301 cacggtcctg ttgcaggggc tgacggcgcc gcacagcgtc gacagtgtcg cccgccgcat
      361 cctgcaggtg ctctccgagc cgatcgcgat cgacggcgag tcggtcctga ttacgccgag
      421 catcggcgtc gcgatcacca cacccgacct cgacgatacg tccgaagggc tgctgcgggc
      481 tgccgacacc gccatgtatc gcgccaagcg cgacggcaag gcgcgctacg ccgtcttcag
      541 tcgggatggc agcgatgatg acgacacgct ccagctggtc aaggccagct gaacactggc
      601 ctgcggcaac acagcaacga cccggagcgc aacgctccgg gtcgtttgga tcagtgcttc
      661 gatacgtcag gcgatgaaga acacgatcgc gagcacgatc agaaccacca tcaccgggag
      721 gagaactgag cgccgaagaa gtgaggacat tgtcgtctcc ttactggttg tacgtcgctg
      781 gtcaacatcg accatggctg tagtacaccc gatttggagg cgctgtgtag acgcgtgcga
      841 tacggcgtgg gtgtaatcca cgattcgctg acccaatccc aaatcaatac ctcaatccgg
      901 tacgggcgat atcgcaatat cgccctgctc gacgcccggc gctttgagaa gggcgatatt
      961 gcgatatcgc ccgtaccgga ttgacgattg aggattgacg cgacagggcc gggtgcatgc
     1021 acccggccct gtcggctgtg atgggttgtc gcgatccggc ttactcggct ggtgtcgcga
     1081 agacggccag cgtttcggag aggatcgcgg actgccagaa gtagttgagc gcgctgagct
     1141 gatcgtccgg ggttccggag cgcagcaggc gggcgttgtc gtaatagtag agcgactgga
     1201 ccggatcctc gccggcgacg gcgccgagcc actgctgggc gcgctggtcg gccaggtcga
     1261 gcatgtcgac cagcgacgac tgacggtcgt agccgacgac cgagccggac tcgtcaacct
     1321 gggccgagag cgagtagtac ttcgcgatca gcgaggccga ctgggagtag gcggtcagcg
     1381 ccgagccgag gttgacggac gcttgtgcct cgggcttgag cattgtgccg gccagcgacg
     1441 aggcgccggc gacggcggcg acggccgtca ggtagctgtt gtcggactgc tggaagtagt
     1501 acttggccag gtccgggtgg ataccgtact gctgcgccca tgggtcgatg atgagcgact
     1561 cgaagtaggt gatgttggcc tcggcgccac gccgcagcgt ctccgagatg gccgtcagca
     1621 cttccaggct cggggccggc gagttgccga agccgagacc gatcccgagg ccgtcttcgg
     1681 ccaggtcgag atacatgtcg gcgtagacgt agttgtagtt ggcggcgtag atggccgtca
     1741 gcgcgtcgtc ctcggtcgcg tccgggttgt tggcgtagtc gttcagggcg gcgtcggcct
     1801 ggaagatgag gccctgggcg accgagaggt tcgagaaggc gtccatgatc gcgatcgagt
     1861 cggcggccgt gcgcggggtc tcggcttcga gccgctgcga gacggccgac atgcgcgttt
     1921 gggctgatga gctggagttc aggcgatcga cgagcgggcc gatgccctgc tggtagtaga
     1981 tgttgtccag ctcggcggcc tcgcgggcga cccgcgccag ggcggcggcc gtgaaggcgt
     2041 tctcgtaggc agcggcgatc tgaccctcgc cgaggtactg gtcggccatg ccggcgtaat
     2101 agtcggcggc ctggatcgtc tcgaggcggt actcctggac gtcagccggg agggcgtaga
     2161 agtcgttgac ctcggactga taggtcgcgt accagttggt cgcgccggcg cggtacttgt
     2221 cgaacgccga cgacgggaag gctgcctggc cggtgttggc cggggccggc agctcgctgc
     2281 cggtcagctc gcggtaggca tcgtagatat ccgagacgag cttgacctcg acgccgttct
     2341 gctggccgac ctggaccagg tcaaccgact ggccgagggc gtagtcgtag tcgtagcgct
     2401 ggccgcccgg aacgaggacg gtcgtcttgc cggcggctgc cgcaccttcg atcttgtgcg
     2461 ggatgccgcc aaccggaccg atcgtgccgt cggggttgat cgtgccggtc atcgtcacgg
     2521 tcgggtcgat ctcgtcgccg aggtagccgg ccaggacggc gatcgtcatc agcgcgccag
     2581 ccgacgggcc gtcgattcgt ccggcgacgt cgaacgagaa ctcatagtgc gacgggtcgt
     2641 tgccgatcag cagcgaggcg gtgatgacgg ccatccagcc ggcggcgcgc cactgcgggc
     2701 cggagccacc gacttcggac tcgaagaatc caacgcgcag gtcctggtca tccttcgagt
     2761 tgaccgagac gacgacccgg ctggtgccgc cctggacgga ctggccgtcg ctggcgaacc
     2821 agagcgcgtc aaccgcgacc ggctcgccg
//
LOCUS       DASUGJ010000665         8690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1081314, whole genome shotgun sequence.
ACCESSION   DASUGJ010000665 DASUGJ010000000
VERSION     DASUGJ010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081314"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..751)
                     /locus_tag="VFV93_15110"
     CDS             complement(248..751)
                     /locus_tag="VFV93_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166732.1"
                     /translation="MTTPDDRADNGVAPDQSPAVDREIANILIVEQDADCADALEAIL
                     ADLPGIATVQTAETTGDALSLLNDAEDDVVGFGIACVIPPAPDVIFIGADQPDSNDVA
                     FELLQTLTTLRRRVPDASIVLLCVYPSQYRDVLGDLIDGCIRKDTSARELHMLIDDLR
                     AAHVYER"
     gene            complement(1161..1895)
                     /locus_tag="VFV93_15115"
     CDS             complement(1161..1895)
                     /locus_tag="VFV93_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166733.1"
                     /translation="MKTRTDGEQAVPARQGIQRQLRRARGARLLIALLMLAALVLGAC
                     ADDNAERASTGPTSSAAEPIVTASPRQQAQSATATATGKAATSTRVATQKAATPAATR
                     TPARTPTPIPPPEPAIGDTIETDGWKITITGFDLYQTVGDSNASGVFLYLRMSIENTA
                     DQPRAFPFDGLVVLDVNDQSYFLAEAPTRETLTYDFGLEIDGQINAHEGRNVAAVFDI
                     APDATGLVLTTPSRVFQIRIEYDEPK"
     gene            2466..3677
                     /locus_tag="VFV93_15120"
     CDS             2466..3677
                     /locus_tag="VFV93_15120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166734.1"
                     /translation="MVVARPAAGARWQFGLGRENGLLFWAVTFLEAAFGSYWALWPIW
                     IEELNAPITLVGLLLGLGGILRLFVLLPSAWLSRRYGLKRVMIVARSIATIGIFSAAL
                     APSWPWLLPALLGMAVGDLAFPLASTFVARNAGERRVRAFAIIFTTGPSTSLLITPLL
                     SGALVATLGLRAPFVAAAIFSLISIALMSRLRTVPPPDDDDSSTGGYRGLLRVPGLPL
                     LLGVQFLTFFAIGIGTSLLSIYLHEAMGYGEAVIPPMTALTAIGSITFSTIVARSSRL
                     SGSPLRAVAIMVGVTASAFLLFLRADLLPFALLAMTLRGGFFSAWPLYSAALGEMTPD
                     RLRPHVFAMCEILAGTGFVLAPVVAGPLYEIQPELPLLLAAAMVTPLALLIGARANTS
                     TEPAVDAGVNQ"
     gene            complement(3760..5436)
                     /locus_tag="VFV93_15125"
     CDS             complement(3760..5436)
                     /locus_tag="VFV93_15125"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5166735.1"
                     /translation="MSEPDPESDIETEIERLRRENELLRRELAARDGGLLQAGGMALL
                     AEGSRIFAETLDREQTLEAVARHLATTLGDACLIWLLTDDGEWRRLVAIDHRDPERLA
                     RMRSHLPDDPRHPARGWSLLDGQTDIYPVVPAEDLPLRIWEPLLPYAREHPVHSIIAA
                     PLRVRGRVIGAIRLSRDITPRSYTHDDLLLVEELADHAALAIDNARLYGQARDAQAMY
                     RGLFEGVPDAIVVVDDTGQYRDVNAAAETLFGYSRQELLSFRAGGMTEAAGVPEARSH
                     DFFASLRHDGYWRGESEVRRGDGSIIPVEGHATAVELPEGRMYIAVSRDVSERRALER
                     MQQEFISMVTHELKIPLTTMRGFVQLMLRRKQLSEPALRTMLAQADRLERLINDLLDS
                     ARLEAGRLEIVPERVDLIALARETAQAEVQLSPTHTIRVLAPETRVVGRWDAGRLEQV
                     FENLLTNAIKYSPDGSEVIVRIEVSGAEAIVTVTDQGSGIPPEQLPHLFSRFTRLHAA
                     AASGTPGLGLGLYIARSLVEAHRGRIWVESEVGSGSTFGFALPLEYPLSS"
     gene            complement(5649..6776)
                     /locus_tag="VFV93_15130"
     CDS             complement(5649..6776)
                     /locus_tag="VFV93_15130"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5166736.1"
                     /translation="MGIYETLGVRRVINADARLTRLGGSLMPEPVLRAMEAAATSYVD
                     MIELQRAVGRRLAELTRNEAAYVSTGAATGLFLTTLACMTGPDPAAIERLPKLDGLKN
                     EVLFQRVQHFAYLPAVPLSGATLVEIGSEPEMHPAELEAAISERTAAILYVAGEHMAA
                     GALPLPEVIEIAHRRGVPVIVDAAAQLPPAANLWYFTRELGADLVIFSGGKDLRGPQA
                     SGLIVGRPDLIEACLLNGAPNPRLGRPMKVGKEEMLGLLAAVELYLAEDEPARIERFE
                     AIVRDWISTFDGRTGVRAWRQFPNEAGQPMPRCVVEVDAAITGLTGAELVRRLWESDP
                     RIAVKQQGETGISMTPDTLSEGDEHIITERLLEIICSPLSV"
     gene            complement(7130..8140)
                     /locus_tag="VFV93_15135"
     CDS             complement(7130..8140)
                     /locus_tag="VFV93_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166737.1"
                     /translation="MFVDVETFLIAVYTLVDDLYQAHLASGKPTRRGCPPAVSDSEVL
                     TLMLLGHWLGNSERGLLRHAHAYWRAYFPRLLSQRAFNRRARRLGSTCAALLQLVAHE
                     LQAETSAYQVADTVPVPLAQLCRGKHHRLFANDAAIGRGGSDKHFYYGQALLLAVSAE
                     GVITGFVAGPASTEGRWLLDALLTWRANPAASPWTVADVPTERKRRGGYVGPTGPRWW
                     PGTAGAAATVPYIADDGFCGAAWVRHWHDDLAATVFTGRGYGPTAPAHLTRSHHRWRQ
                     VIETVNESLNAALHLPCPRAKTPWGVITRLTAKCCAFNVGIWVNRLLGRAPLAIDTLF
                     PA"
BASE COUNT         1424 a         2949 c         2844 g         1473 t
ORIGIN      
        1 gcgcgcacca gccaacgcgg caacggcatg caggtggtca cctagcgggt gccactgacg
       61 cgtagttcct ggcgtgtgag cataaagttg ccgcgtgtcg cttccgtcaa ctcccatgca
      121 atctcctgcc atgcctatac cgcaggcatc gacacgatac acgaattgtc ttatgcatgg
      181 gagtatagca gccggaaact acatcgggga gacctaccgg gtacgctccg caggcaacgc
      241 gtctcgacta gcgctcgtat acgtgggcgg cgcgcaggtc gtcgatcagc atgtgcagtt
      301 cgcgggcgct ggtgtccttg cggatgcagc cgtcgatcag gtcgccgagc acgtcgcgat
      361 actggctcgg gtagacgcag agcagcacga tcgacgcatc cgggaccctc cggcgcaacg
      421 tcgtcagggt ctgcagcaac tcgaaggcga cgtcattcga gtccggctgg tcagcgccga
      481 tgaagatcac atccggcgcc gggggaatga cacaggcgat cccaaagccg acgacatcgt
      541 cctcggcgtc gttgagcaac gagagcgcat cgcccgtcgt ctccgctgtc tggacggtcg
      601 cgataccggg caggtcagcc aggatcgcct ccagcgcgtc ggcgcagtcg gcgtcctgct
      661 cgacgatcag gatgttggcg atctcgcggt cgaccgccgg cgactgatcc ggcgcgacac
      721 cgttgtcggc ccgatcgtca ggtgtggtca tcaagttcaa cagcttgcgc atcggtctgc
      781 tcatctcgtc actggtcccg ggcgacagca tcgtcgccgt gggtattgcg tcggtacgcc
      841 gcacgtcatt gtgcgtacca ccagcatagg ctggggcagc cgaggcaacc accgttgaac
      901 gttcggactc acggcagcac taccggccaa ttcccgactg gccaggtgga cagggtgagg
      961 atgagagcaa ggactgaggg agtggcaaaa cgcgcgagtg gaagctgaaa ggggaacgtg
     1021 aaagcggacc gtgaggcggg gggaacacac cgcacccatc gataccctga gaacgcgacc
     1081 gccgcgccct cccctcactt tcccctttca gcttccaccc acaccatcta cccggggcgc
     1141 cgcgccggcg ctacccggcc ctacttcggc tcgtcgtact cgatccggat ctggaacacg
     1201 cgcgacggtg tcgtcagcac caggccggtc gcatccggcg cgatgtcgaa gactgctgcg
     1261 acgttgcggc cttcgtgcgc gttgatctgc ccatcgatct ccagaccgaa atcgtaggtc
     1321 agcgtctcac gcgtcggcgc ctcggccagg aagtagctct ggtcgttgac gtcgagaaca
     1381 accagaccgt cgaacgggaa agcgcgcggc tggtcggccg tgttctcgat cgacatgcgc
     1441 aggtagagga agacgccgct ggcgttgctg tcaccgaccg tctgatacag gtcgaacccg
     1501 gtgatcgtga tcttccagcc gtccgtctcg atcgtgtcgc cgatggccgg ctccggtggc
     1561 gggatcggcg ttggcgtccg ggccggggtg cgggtcgcgg ccggcgttgc ggccttctgg
     1621 gtcgcgacgc gggtggacgt tgcggccttg ccggtggcag tggccgttgc cgattgcgcc
     1681 tgctgccgtg gtgaggccgt cacgatcggt tccgcggcag acgatgttgg gccggtgctg
     1741 gcccgctcgg cattgtcgtc ggcgcaggcg cccagcacca gtgcggccag catcaacagc
     1801 gcaatcagga gccgcgcccc tcgcgctctg cgcaactgcc gttgtatccc ctgcctggct
     1861 ggcaccgcct gctcgccatc agttcgtgtc ttcatcggac catcccatcc ctgcacgagc
     1921 gagattcggc gtcccggcaa tgaagttgag cgtggtgtac gtccataatg ctcgtacgcc
     1981 gcccaaaact cgctgtgaca acatgacaac tcttgcagac ttgcgagaga tatcgttcat
     2041 gtcactcaga caatacactg ccggcgatga cgcgtcaacc gccagcaacg cgtctggacg
     2101 cggaaatccg gccgcagacg cagcctgatc gtgctcgatt gttggacctc gggatctgtc
     2161 tgaatgcacc gcgcagcgaa ggggatcgtg gcacgatcgc cacgatcgaa ctcgcggcgc
     2221 ggtgcgaacg cgcgagtgta cggacatctt accgggtcgc tggcccagcg cgacccggca
     2281 agactcgccg cctgctccag tcctgcccga acaggatcgg tcctcaagcg acggcccagg
     2341 cgcgttcgcg gcggtatgcc cagccattac caatcgcgca aatgcagcct gcccaccgca
     2401 ccagcatgga catccggctc cggcgcggca atgccataat ggcgggcgac gggaagggaa
     2461 caggaatggt cgttgcgagg ccggcggctg gcgcacgctg gcaatttggg ctgggccgcg
     2521 aaaatgggtt gctcttctgg gccgtgacgt tcctcgaagc ggccttcggc tcgtactggg
     2581 cgctctggcc gatctggatc gaggagctta acgcgccgat cacgctggtc gggctgctgc
     2641 tcgggctggg cggcatcctg cggctcttcg tgctgctgcc gtcggcctgg ctctcgcgcc
     2701 gctacggcct caagcgcgtc atgatcgtcg cccgctcgat cgcgaccatc ggcatcttca
     2761 gcgccgcgct ggcgccgagc tggccgtggc tgctgccggc gctgctgggg atggcagtcg
     2821 gcgatctggc attcccgctg gcatcgacct tcgttgcccg caacgccggc gagcggcggg
     2881 tgcgcgcctt cgccatcatc ttcacgaccg ggccgtcgac ctcgctgctc atcacgccgc
     2941 tgctctctgg cgcgctagtc gcgacgctgg ggttacgggc gccgttcgtg gcagccgcca
     3001 tcttctcgct gatctcgatt gcgctgatgt cccgcctgcg aaccgtgccg ccgcctgacg
     3061 acgatgacag cagcacgggc ggctatcgcg ggctgctgcg tgtgccgggg ctgccgctgc
     3121 tgctcggggt ccagttcctg accttcttcg cgatcgggat cggcacctcg ctgctctcga
     3181 tctacctgca cgaggccatg ggctacggcg aagccgtcat cccaccgatg acggccctaa
     3241 ccgcgatcgg ctcgatcacg ttctcaacga tcgtcgcccg cagcagccgg ctcagcggca
     3301 gcccgctgcg cgccgtcgcg atcatggttg gcgtgacggc cagcgccttc ctgctcttcc
     3361 tgcgcgcgga tctgctgccg ttcgcgctgc tggcgatgac gctgcgtggc ggcttcttct
     3421 ccgcctggcc gctctattcg gctgcgctgg gcgagatgac gccggatcgc ctgcgcccgc
     3481 acgtcttcgc gatgtgcgag atcctggccg gcaccggatt cgtcctcgcc ccggtcgtcg
     3541 ccggcccgct ctacgagatc cagccggaac tcccactcct gctggcggcg gccatggtca
     3601 ccccgctggc gctcctgatc ggtgcacggg ccaacacatc gacagagccg gccgtcgatg
     3661 cgggagtgaa tcagtaaccc tgtcgtcctg agccgcagcg aaggacctct ccgccttaac
     3721 agcgcgcgag gctcacgcgg agaggtcctt cgctgcggct caggacgaca gggggtactc
     3781 cagtggcaag gcgaagccga aggtgctgcc ggaaccgacc tcgctctcga cccagattcg
     3841 gccgcgatgg gcttccacca gcgagcgggc gatgtagagg cccaggccga ggccgggtgt
     3901 gccgctggcc gctgcggcgt gcaggcgggt gaagcggctg aagagatgtg gcaactgctc
     3961 gggtgggatg ccgctgccct gatcggtgac ggtgacgatt gcttcggcgc cgctgacctc
     4021 gatcctgacg atgacttcgg agccgtccgg cgaatacttg atcgcgttgg tcagcaagtt
     4081 ctcgaagacc tgctccagcc ggcccgcgtc ccagcgtcca acgacgcgcg tctccggtgc
     4141 gaggacccgg atcgtatggg tcgggctgag ctggacctcg gcctgagccg tttcccgggc
     4201 cagcgcgatc aggtccaccc gctccggaac gatctcgagc cggcccgctt cgagccgggc
     4261 gctgtcgagc aggtcgttga tcagccgttc cagccggtca gcctgggcga gcatcgttcg
     4321 cagcgccggc tcgctcagtt gcttgcggcg cagcatcagc tggacgaagc cacgcatcgt
     4381 cgtcagcggg atcttcagct cgtgggtcac catcgagatg aattcctgct gcatccgttc
     4441 cagcgcgcgc cgctccgaga catcgcgcga gacggcgatg tacatccggc cttcgggcag
     4501 ttcgacggcc gtcgcgtgtc cttcgaccgg aatgatgctg ccatcgccac ggcggacctc
     4561 ggactcgcca cgccagtagc catcgtgccg caagctggcg aagaaatcgt gcgaacgggc
     4621 ttccggcact ccggccgcct cggtcatccc accggcccga aacgagagca gctcctggcg
     4681 cgagtaaccg aacaacgtct cggcggcggc gttgacatcg cgatactgac cggtatcgtc
     4741 aacgacgacg atcgcgtccg gaacaccttc gaacagcccc cggtacatgg cctgcgcgtc
     4801 gcgcgcctgc ccgtagaggc gcgcgttgtc gatcgccagc gcggcgtggt cggccagctc
     4861 ctcgaccagc agcagatcgt cgtgcgtgta cgaccggggc gtgatgtcgc gcgagaggcg
     4921 gatcgcgcca atcacccggc ctcgcacccg caacggagcg gcgatgatgc tgtggaccgg
     4981 atgctcccgc gcatagggca gtagcggttc ccagattcgt agcggcagat cctcggccgg
     5041 aacgaccgga tagatatccg tctgcccgtc cagcagcgac cagccacggg cggggtgacg
     5101 tgggtcatcg ggcagatgcg agcgcatccg cgccagccgc tccgggtcgc ggtgatcgat
     5161 cgctaccagc ctgcgccact cgccatcatc cgtcagcagc cagatcaggc aggcatcgcc
     5221 caaagtcgtc gcgaggtgcc gggcgaccgc ttccagcgtc tgctcgcggt cgagcgtctc
     5281 cgcgaagatc cgcgatcctt cggccagtag cgccattcca ccggcctgaa gcagaccgcc
     5341 gtcgcgcgcg gccagttcac ggcgcagcag ctcattctcg cgccgcagcc gctcgatctc
     5401 ggtttcgatg tcactctcgg ggtcaggctc ggacatcggc gtgggccatt cacggcaaac
     5461 agatcaacca acagacagcg cccggtcagt cagcgtcacg gtgagagagg ctaccggtcg
     5521 cgccggcagc cggcacaccg cgatggtgtc ggcccaacgc cgaacgacta cactccacaa
     5581 ccgcagatcg tgccgctatc gtagcccgat cgttccgcgc ccgtcaacgc accaaccaag
     5641 ggcggtgcct acacggatag gggagaacag atgatctcca gcaagcgttc ggtgatgatg
     5701 tgttcgtcgc cctctgacag cgtgtcgggt gtcatcgaga tgccggtctc accctgctgc
     5761 ttgacggcga tgcgggggtc gctctcccag agccggcgga ccagttcggc gccggtcagg
     5821 ccggtgatgg ccgcgtcgac ctcgaccacg cagcgcggca tcggctggcc ggcctcgttg
     5881 gggaactgcc gccaggcgcg aacgccagtc cggccgtcga aggtactgat ccagtcgcga
     5941 acgatcgcct cgaagcgctc gatgcgggct ggctcgtcct cggccaggta gagctcgact
     6001 gccgccagca ggccaagcat ctcctccttg ccgaccttca tcggccggcc aagccgtggg
     6061 tttggcgcgc cattgagcag gcaggcctcg atcaggtcgg ggcggccgac gatcagcccg
     6121 ctcgcctgcg ggccgcgcag gtccttgccg ccgctgaaga tgaccaggtc cgcgccaagc
     6181 tcgcgggtga agtaccacag gttcgcggcc ggcgggagtt gcgccgccgc atcgacgatg
     6241 accggcacac cccgtcggtg cgcgatctcg attacctccg gcagcggcag cgccccggcc
     6301 gccatgtgct cgccggcgac gtagaggatc gccgccgtcc gctcgctgat ggccgcttcc
     6361 agctcggccg ggtgcatctc cggctcggag ccgatttcga ccagcgtcgc acctgacagc
     6421 gggacagccg gcagatacgc gaaatgctgg acccgctgga acagcacctc gttcttcagc
     6481 ccatccagct tcggcaagcg ctcgatcgcg gccgggtccg gcccggtcat gcaggccagt
     6541 gtcgtcagga acagcccggt cgccgcgccg gttgagacgt aggccgcctc gttgcgcgtc
     6601 agttccgcca gccgtcgccc aacggcccgc tggagctcga tcatatcgac ataactggtg
     6661 gccgcggcct ccatcgcccg cagcaccggc tccggcatca acgacccacc cagccgcgtc
     6721 aagcgcgcgt ccgcgttgat cacccgccgc acaccaagcg tctcgtagat acccatcgtg
     6781 agccgctgtc cttccctcgc tgctggttgc catgagtcgt cggcgcactc taccggttat
     6841 gcggtgtcgt gtcacctggc acacggtcgg ctcacatcga cggctcgcat ttggtgacgg
     6901 ggtggggtga ggagcgaagc gtggtgcgtg tcgtggcgag ggtgagctac gaggtgggga
     6961 gtcaggcggg gtacatcgtg cggcgagggg gcaggtacgg aggggcagcc gggcggggtg
     7021 cgcgtcgtgg cgagggagac ggagtggggt ggggagccgg gcgggtcttt cgacgtgtca
     7081 ggtgtcaagc tgaccataac gacccgcccc tactgacggc tgatgcagat tacgccggga
     7141 agagggtatc gatcgccagc ggcgcgcgac cgagcaatcg gttgacccag atgccaacgt
     7201 tgaaggcgca gcacttggcc gtcaggcgcg tgatgacccc ccacggtgtt ttggcgcgtg
     7261 ggcagggcag atggagtgca gcattcaggc tctcgttgac cgtctcgatc acctgtcgcc
     7321 agcgatgatg cgaccgtgtc aggtgggctg gcgcggtcgg gccatagccg cggccggtga
     7381 agacggtcgc cgcgaggtcg tcatgccagt ggcgcaccca ggcagcgcca cagaagccat
     7441 catcggcgat gtacgggacc gtggcagccg cgccagccgt gcccggccac cagcgcggac
     7501 cggttgggcc gacatagcca ccgcgacgtt tgcgctccgt cggcacatcg gccaccgtcc
     7561 acgggctggc ggcgggattg gctcgccagg tgagcaatgc atccagcagc cagcgtcctt
     7621 cggtgctggc tggcccggcc acaaagccgg tgatgacccc ctcggcgctg actgccagca
     7681 gcagcgcctg tccatagtag aagtgtttgt cactcccacc acgcccgatg gcggcatcgt
     7741 tggcaaacag ccgatggtgt ttgccacgac acaactgggc cagtggtacc ggcacggtgt
     7801 cggcgacctg atacgcgctg gtctcagcct gcagttcgtg ggcaaccagc tgcagtaggg
     7861 cggcacaggt gctgcccaac cgtcgcgctc gccgattgaa ggcacgctga ctgagcaggc
     7921 gcgggaagta cgcgcgccaa tacgcatgcg cgtgacgcaa caggccacgt tcactgttgc
     7981 cgagccagtg tcccagcagc atcagtgtca gcacctcgct gtcggacacc gctggcggac
     8041 acccacgacg ggtcggtttc ccactggcca gatgcgcctg atacagatca tcgaccagcg
     8101 tgtagactgc gatcaggaag gtttcgacat cgacgaacat ggctggctcc ttgcacgatc
     8161 acgccatttc ccaatgtcgg aaccttcatc gacttccgtg ccagcaccac gatttctgca
     8221 tcagccgtta ctgagccgcc tcggcgtcgc gtaccgttgc gagaggccaa ccgccgttgt
     8281 cgaggagcac gcgcgacaag taggggcggg tcgtcgcgac catctcgacc cttgacccat
     8341 cgaaagaccc gcccggctcc cgcacctcgc acgttcgctc gcgtcgacac gcatcccacc
     8401 cggctccccc atccaacccg gtcaccctcg ccacactggc gcacatccgc ccggctcccc
     8461 cacccaaacc ccgtcagcct cgtcatctga ccggtagctg acgattgcgc cgatagccgc
     8521 gttgcacaca gcacagacca gctctggccc gcgctacgga taggcatggc cgggcgagag
     8581 tagcctcagg acgtgctcgg cttcttcgaa gtggtgagcg acatcgacga ccgtcatgac
     8641 atcaatccag acgcgcggtg tcgtccagcg caggaacgct tcgcgcagca
//
LOCUS       DASUGJ010000666         4910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_248688, whole genome shotgun sequence.
ACCESSION   DASUGJ010000666 DASUGJ010000000
VERSION     DASUGJ010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248688"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..667
                     /locus_tag="VFV93_15140"
     CDS             314..667
                     /locus_tag="VFV93_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5166738.1"
                     /translation="MSMTSWNPTTHDEYDTLHGYDVYSSDNEKLGTIKEVCHPAAAMP
                     AARGQHYFRVEPGTLKKLFTDQDEVFVAETMIREVRPEEDKVILSVPKDRVRDEEWGR
                     PHDFDTYRMGKPSAV"
     gene            896..2269
                     /gene="glnA"
                     /locus_tag="VFV93_15145"
     CDS             896..2269
                     /gene="glnA"
                     /locus_tag="VFV93_15145"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX5166739.1"
                     /translation="MAVEIAPAGRQSEAPSVEQVLETCQREAVQFVNLQFTDVMGIPK
                     LVTIPIDLFEDVIEHGQWFDGSSIAGFARIAESDMFLMPDLATFNIIPWERGRNTTAR
                     VICWVHRPNGDLFEGDPRVVLLRQLERLARLGYAFNTGPELEFFLFRKDGGDISVLPH
                     DRAGYFDLSTDLAAEVRKDMIRALQEMGIKVEAGHHEVAIGQHEIDFEYADALTTADH
                     ATTFKYTLKAIAQQHGLHATFMPKPVEGIAGSGMHTHQSLSWLDGGGNAFIDADDAYG
                     LSTLAKHFIAGQLAHARGMTAILNPLVNSYRRLVPGFEAPVYVSWARQNRSALIRVPS
                     VRSGRTAATRIELRCPDPSCNPYLAYAAMLATGIDGIERGLPLPEPVEENLYHFTEED
                     LVRRNVVTLPSTLGEAIDALRADDVIRDALGEHLYDHLTEVQVTEWRAFCQHVTAWER
                     DRYLEVY"
     gene            2436..3110
                     /locus_tag="VFV93_15150"
     CDS             2436..3110
                     /locus_tag="VFV93_15150"
                     /inference="COORDINATES: protein motif:HMM:NF023737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar dehydrogenase complex small subunit"
                     /protein_id="HEX5166740.1"
                     /translation="MPDADDPRISLLTTPLTRRRFVVMSGAVFASSLLTACMGDDDDD
                     SGGGTPTLVGATRTPESAIETPQAGASPTTAASPAASPPASPTGAAGNADLDRFMALS
                     RALTGFDDLDDTELGQVYYDAIANSDTYGDQLDSLYDKAGLDDSATAVTLDQLESAGA
                     FDDDTRPVADAITTAWYSGTYDTGGGDVVVATYINALAWQATGYRLTGPSTCSGATGN
                     WSAAVA"
     gene            3244..4860
                     /locus_tag="VFV93_15155"
     CDS             3244..4860
                     /locus_tag="VFV93_15155"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5166741.1"
                     /translation="MADRLEADVVIVGAGVAGSLIAWTLAQAGAKVLVLESGPWVDRA
                     KAVETYRAAVAKTPEAPYPPLEYAPRPTVAAIDEYYQQQGPDMFQSTYERRVGGTTWH
                     WQGTAMRHLPNDFHIKTLYGVGEDWPISYDDLEPWYVQAEQALGVAGDTDMGSPRSAP
                     YVMPAVPPSYLDLHINAALQPLGLQVLTTPQAKNSQAFDGRPPCCGNNLCVPICPIGA
                     KYDGAVHAKKAQDAGAQIESDSVAHKIEVDGEGRVTRVLFKRPDGSEHEAVGQTFVVA
                     AHAIETPKLLLMSRTDALPNGVANSSDQVGRNLMDHVTQVSLCLSRDPIWPFRAPVET
                     SGIEHLRDGDHRRDWAAFRTPIGNDGWSFGGENPVALSSTLVKDEGLRGTALRDRIQQ
                     MAERQVRLAALVEQLPNPDNRVTLSEDKQDALGNPYPQIAYQVDDYSKRGMEQARALA
                     DQVFDAIGGTDRMHAPTFFGAGHIIGTYRMGNDPATSVVNPQGRTHDHPNLYLAGDGL
                     FPTEATANPTLTLAALALWCADTIKGELGR"
BASE COUNT          875 a         1713 c         1545 g          777 t
ORIGIN      
        1 atggtctccg gctatgaagc gatctacgct ggcgtcctcc accaggacga actgccgtat
       61 accggaccga gcgacgacct catactcgcc gattcgctgt tgcaggaact accagccggc
      121 gaggtggggc tggattgaac cgtgcaggtt cacatctgca cggtcaatcg cgatgccgat
      181 gtcgcgatcc ttggtgcgtg ccggctgaag cagacaggtc ggagttgtca taacggcgtt
      241 tcttggcacg ctacctgcaa tcgatttggc gagggaattc gccctctgga acagctcgga
      301 aaggacaacc tcgatgagca tgaccagttg gaacccgact acccacgacg aatacgacac
      361 gctccacgga tacgatgtct actccagcga taatgagaag ctgggtacga tcaaggaagt
      421 ctgtcatcct gccgccgcga tgccggccgc gcggggccag cattacttcc gtgtcgagcc
      481 gggcacgctc aagaagctct tcactgatca ggacgaagtc ttcgttgccg agacgatgat
      541 ccgcgaggtc cgtccggagg aggacaaggt tatcctcagc gttccgaagg accgcgtccg
      601 ggacgaggag tggggccgcc cgcacgactt cgacacctat cggatgggca agccgtcagc
      661 cgtctagatc accagccgtt tcgccgggcc ggggagcttc cccggccctt gtcgtgtccg
      721 cgattgggca gccggcacaa cgattcccga tcgagttggt acgcgctgcc gtttcaggtc
      781 gcgggccagc ggcgtacgct aacgacgata gcgatgcggg acgttgcccc cgtagggaca
      841 ggcttgaggc ctgtccctac ggatgtcgga tggaactggg acgggagggt cggaggtggc
      901 agttgagatc gcgcctgccg gaaggcagag cgaggcgccg agcgtcgagc aggtgctgga
      961 gacctgccag cgcgaggcgg ttcagttcgt caatctccag ttcaccgacg tgatgggcat
     1021 tcccaagctg gtgacgatcc cgatcgacct cttcgaggat gtcatcgagc acggccagtg
     1081 gttcgacggc tcgtcgatcg ccggcttcgc ccggatcgcc gagagcgaca tgttcctgat
     1141 gccggacctg gcgaccttca acatcatccc ctgggaacgg ggccgcaaca cgaccgcccg
     1201 cgtcatctgc tgggtccacc gcccgaacgg cgatctcttc gagggtgacc cgcgcgtcgt
     1261 cctgctgcgc cagctcgaac ggctggcccg gctcggctac gccttcaaca ctggcccgga
     1321 gctggagttc ttcctcttcc gcaaggacgg cggcgacatc tccgtcctgc cgcacgaccg
     1381 ggccggctac ttcgacctct cgaccgacct ggccgccgaa gtccgcaagg acatgatccg
     1441 cgcccttcag gagatgggca tcaaggtcga agctggccac cacgaagtcg cgatcggcca
     1501 gcacgagatc gacttcgagt atgccgacgc gctgacgact gccgaccacg cgacgacatt
     1561 caagtacacc ctcaaggcga tcgcccagca gcacgggctg cacgcgacct ttatgcccaa
     1621 gccggtcgaa ggcatcgccg gatccggcat gcacacgcat cagtcgctct cgtggctgga
     1681 cggcggcggc aacgcgttca tcgacgccga cgacgcctac ggcctctcga cgctggccaa
     1741 gcacttcatc gccggccagc tcgcccacgc gcgcgggatg acggccatcc tgaacccgct
     1801 cgtcaactcc taccggcggc tcgtgccggg cttcgaagcg cccgtctacg tctcctgggc
     1861 gcgccagaac cgctcggcgc tgatccgcgt cccatcggtc cgttcgggcc ggacggccgc
     1921 gacccgcatc gagctacgct gccccgaccc gagctgcaac ccgtacctgg cgtacgcggc
     1981 catgctggcg accggcatcg acggcatcga gcgcgggctg ccgctgccgg agccggttga
     2041 ggagaacctc taccacttca ccgaggaaga cctcgtccgc cgcaacgtcg tcaccctccc
     2101 cagcacgctc ggcgaggcaa tcgacgccct gcgcgccgat gatgtcatcc gcgacgcgct
     2161 cggcgagcac ctctacgacc atctgaccga ggtccaggtc accgaatggc gcgccttctg
     2221 ccagcacgtc accgcctggg agcgggatcg gtatcttgag gtgtactgaa cgtaggtcgt
     2281 aggtgatagg tgttaggggc tagtatgtgc ggaccagcgt gctgcgctcc tgctgcacga
     2341 accagcccct aacacctatc acctaccccg aggcacgcat tctgcacctc caccggcgag
     2401 acgcgctttg cctcgtactc gtggaggtca cgcctgtgcc tgacgctgac gatccccgta
     2461 tttcgctcct cacgacaccc ctgacgcggc ggcgcttcgt cgtcatgagt ggcgcggtgt
     2521 tcgccagcag tctgctgaca gcctgcatgg gcgacgatga cgatgactcc ggtggcggga
     2581 cgccaacgct ggtcggcgcg acgcgcacgc ccgaaagcgc gatcgagacg ccgcaggccg
     2641 gggcgtcacc aacgacggct gcttcaccag ctgcttcgcc gccggcgtcg ccaaccggtg
     2701 ccgcgggcaa cgccgacctc gatcgcttca tggcgctctc gcgggcgctg accggcttcg
     2761 acgatctgga tgacaccgag cttgggcagg tctactacga cgcgatcgcc aattcggaca
     2821 cctacggcga ccagctcgac agcctctacg acaaggccgg gctggacgat agcgcgaccg
     2881 ccgtcaccct cgaccagctc gaaagcgctg gcgcctttga cgacgacacc cgcccggtgg
     2941 ccgacgcgat cacgaccgcc tggtacagcg gcacctacga taccggaggc ggcgatgtcg
     3001 tcgtcgccac ctacatcaac gcgctcgcct ggcaggcgac cggctaccga ctcaccggcc
     3061 ccagcacctg tagcggcgca accggcaact ggagcgcggc agtagcgtga gaggcgccag
     3121 gtgccaggtg ccaggtgcca ggagcttgtt gctgggattc gaccggatgc gttttccctg
     3181 cgctaaccaa cccctaacac ctatcaccca atacccatca cctcgcaacg aacggagacc
     3241 agcatggcgg acaggctcga agcggatgtt gtcattgtcg gggcgggggt ggccgggtcg
     3301 ttgatcgcct ggacgctggc gcaggccggg gcgaaagtgc tggtcctcga atcaggtccg
     3361 tgggtcgatc gggccaaggc cgtcgagacg taccgcgctg ccgtcgccaa gacgcctgag
     3421 gcgccctacc cgccgctcga gtacgcgccg cgtccgaccg tcgccgctat cgacgagtac
     3481 taccagcagc aggggccgga catgttccag agcacctacg agcggcgcgt cggtgggacg
     3541 acctggcact ggcagggaac cgccatgcgt cacctgccca acgacttcca tatcaagacg
     3601 ctctacggcg tcggtgagga ctggccgatc agctacgacg acctcgagcc gtggtatgtc
     3661 caggccgagc aggcgctggg cgtcgccgga gacaccgaca tgggctcgcc ccgctccgcg
     3721 ccgtacgtca tgccggccgt cccacccagc taccttgatc tgcatatcaa cgccgccctc
     3781 cagccgctcg gactccaggt gctgacgacg ccgcaggcca agaactcgca ggcgttcgac
     3841 ggccgcccgc cctgttgcgg caacaacctc tgtgtcccga tctgcccgat cggcgcgaag
     3901 tatgacggcg ccgtccacgc caagaaggcg caggacgccg gtgcccagat tgagtcggac
     3961 tcggtcgctc acaagatcga ggttgatggc gagggtcgcg tcacccgcgt cctcttcaag
     4021 cggccggacg gcagcgagca cgaggccgtc ggccagacgt ttgtcgtcgc cgcccacgcg
     4081 atcgaaacgc cgaaactctt gctgatgtcg cgcaccgacg cgctcccgaa cggcgtcgcc
     4141 aactccagcg accaggtcgg ccgcaacctg atggaccacg tcacccaggt cagtctctgc
     4201 ctctcgcgcg acccgatctg gcccttccgc gcgccggtcg aaacctccgg catcgagcac
     4261 ctgcgcgacg gcgaccaccg ccgcgactgg gccgccttcc ggacgccgat cggcaacgac
     4321 ggctggtcgt tcggcggtga gaacccggtc gcgctctcct cgacactcgt caaggacgaa
     4381 gggttgcgcg ggacagccct gcgcgatcgc atccagcaga tggccgagcg gcaggttcgg
     4441 ctggcggcgc tcgtcgagca gcttcccaat cccgacaacc gggtcactct ctcggaagac
     4501 aagcaggatg cgctcggcaa cccgtacccg cagatcgcct atcaggtcga cgactacagt
     4561 aaacgcggca tggagcaggc gcgggcgctg gccgatcagg tcttcgacgc gattggcggc
     4621 accgaccgca tgcacgcgcc aaccttcttt ggcgccggcc acatcatcgg cacctatcgg
     4681 atgggcaacg acccggccac ctcggtcgtc aacccccagg gccgcacgca tgaccatcct
     4741 aacctctacc tggccggcga cggcctcttc ccgaccgaag ccaccgccaa cccaaccctg
     4801 accctcgccg cccttgccct ctggtgcgcc gacacgatca agggtgagtt ggggcggtag
     4861 tgtgggggac gcggggcgcg gggcgcggga cgtgggacgc gatccagacg
//
LOCUS       DASUGJ010000667         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_943179, whole genome shotgun sequence.
ACCESSION   DASUGJ010000667 DASUGJ010000000
VERSION     DASUGJ010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943179"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFV93_15160"
     CDS             complement(<1..309)
                     /locus_tag="VFV93_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166742.1"
                     /translation="MLDSLPAVSRSPVVDAGRPGSAPDVLVIKTSSLGDVIHQMPALT
                     EAQRRLPQARFSWLVEEAFAPLVRLHPAVAEVIPVATRRWRRSIYIPETWREMANAAR
                     A"
     gene            complement(309..770)
                     /locus_tag="VFV93_15165"
     CDS             complement(309..770)
                     /locus_tag="VFV93_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166743.1"
                     /translation="MHTILSLVALLAGLVVVAGLLGTRTLPQWTGLFLVTAIATSVTG
                     FGFPFEKFEASHWIGVISLVALAVAILARYMFHLDGAWRWIYVVTITVAAYFLVFVTI
                     AQAFKKVPRLHAMAPTLSEAPFTVSQLLVLVVFAGIIAAAVIKFRPPATRA"
     gene            complement(953..1318)
                     /locus_tag="VFV93_15170"
     CDS             complement(953..1318)
                     /locus_tag="VFV93_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5166744.1"
                     /translation="MSRLILVVEDQEDNRRILRDLLTSANYELVEASTGEEALAALAK
                     RPPDLILMDIQLPVMDGYEATRRIKADPATAAIPIIAVTSYALSGDEEKARAAGCDDF
                     VPKPYSPRQLLEKIQQYLP"
     gene            complement(1336..>2399)
                     /locus_tag="VFV93_15175"
     CDS             complement(1336..>2399)
                     /locus_tag="VFV93_15175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5166745.1"
                     /translation="VAGNQVEADHIRYLADRPVAVDRGSVTGRVVLERRTVHVPDVLA
                     DPEFDQFEWQRVGKQRTVLGVPLVREDALIGVIILARTQVEPFDERQIDLVTTFADQA
                     VIAIENVRLFDEIQEKSRQLAEASQHKSQFLANMSHELRTPLNAILGYTELIMDGIYG
                     DTPDRMRDVLDRVQKNGRHLLGLINDVLDLSKIEAGQLTLALSSYSLKDVVHGVYSAV
                     EPLASEKKIALRIELPQELPQGRGDERRLTQVLLNLVGNAIKFTDQGEVVVKASATNG
                     SFNLSVRDTGPGIGEKDQARLFQEFQQADNSITKKKGGTGLGLAISKRIIELHGGKIW
                     VESQLGQGSTFSFTLPVTV"
BASE COUNT          459 a          780 c          735 g          425 t
ORIGIN      
        1 cgctcgcgcg gcattcgcca tctcgcgcca agtctccgga atgtagatcg agcggcgcca
       61 acgcctggtc gcgaccggga tcacctccgc gaccgccggg tgaagccgca ccagcggcgc
      121 gaacgcctcc tcgaccagcc aggagaatcg cgcctgcggc agccggcgtt gcgcttcggt
      181 gagcgccggc atctggtgga tcacgtcgcc cagcgaagat gtcttgatga ccagaacgtc
      241 cggtgcggaa cccggacgcc ccgcgtcgac aacgggtgag cgcgacacgg cggggaggga
      301 gtcgagcatc aagcccgcgt cgccggtggg cgaaacttga tcacggcggc ggcgataatg
      361 ccggcaaaga ccacgagaac gagcaattgc gacaccgtaa acggcgcctc cgacaaggtc
      421 ggcgccatcg catgcaagcg cggcaccttc ttgaacgcct gcgcgatcgt gacgaagacg
      481 aggaaatacg ccgccaccgt gatcgtcacg acatagatcc agcgccaggc gccatcaaga
      541 tgaaacatgt agcgcgcgag gatcgcgacg gcgagcgcga ccagcgaaat cacgccgatc
      601 caatgcgagg cctcgaattt ctcgaacgga aatccaaagc cggtgacgct tgtcgcgatc
      661 gcagtcacga ggaagagccc ggtccactgt ggcagcgtcc gggtgccgag cagaccggcc
      721 accacgacca accccgccag cagcgctacg agactcagga tcgtgtgcac ccaggtaaac
      781 gtcgagaacc caaggatcat ggcggccgcc tcattcgttt cgatcagact cgccgtgtgc
      841 gcgtttaccg gtgagccgct ctgcgccgag agatagtgtg caccgaactg gttgcgccgt
      901 ccttaagcca agcggcgcgc tcctgtcgag ggctcggcgc gcaatccggc tatcacggca
      961 aatactgctg gatcttctcc agcaactggc gcgggctgta cggcttcgga acgaagtcgt
     1021 cgcagccggc ggcgcgcgcc ttttcctcgt cgccgctgag cgcgtacgat gtcaccgcga
     1081 taatcggaat ggcagcggtt gccggatcag ccttgatgcg tcgcgtcgcc tcatagccat
     1141 ccatgacagg tagctggata tccatgagga tcaggtccgg cggcctcttc gccagcgccg
     1201 ccagcgcttc ctcgccggtg ctcgcctcga ccagttcgta gttggcgctg gtcaacagat
     1261 cgcggaggat acggcgatta tcctcctgat cctcgactac cagaatgagc ctgctcatcg
     1321 tcgtcgtgtc cgtactcaaa cggtgaccgg cagcgtgaag gagaatgtcg agccctgccc
     1381 tagctgcgac tcgacccaga tcttcccgcc gtgcagttcg atgatgcgct tggaaatcgc
     1441 gagcccaagg ccggtgccgc ccttcttctt ggtgatggaa ttgtcagcct gctggaattc
     1501 ctggaacagc ctggcctgat ccttctcgcc gatgccgggt ccagtgtcgc gcaccgagag
     1561 attgaaagag ccgttggtcg cgctcgcctt gaccaccacc tcgccctggt cggtgaactt
     1621 gatggcatta cccacaagat tgagcagcac ctgggtcagt cgccgctcgt cgccgcggcc
     1681 ctgcggcaac tcctgcggca gttcgatcct gagggcgatc ttcttctccg aggcgagcgg
     1741 ctcgaccgcg ctgtagacgc cgtgcaccac gtccttgagc gaatagctcg acagcgcaag
     1801 cgtcaactgg ccggcctcga tcttggaaag gtccagcacg tcgttgatca ggccgagcag
     1861 atgccggccg ttcttctgga cgcggtcgag cacgtctcgc atcctgtccg gcgtgtcgcc
     1921 gtagatcccg tccatgatca gctcggtgta gcccagaatg gcattgagcg gggtgcgcaa
     1981 ctcgtgactc atgttggcca ggaactgcga cttgtgctgg ctcgcttcgg cgagctggcg
     2041 gctcttctcc tggatctcat cgaacagccg cacgttctcg atcgcgatga ccgcctggtc
     2101 ggcgaaggtc gtgactaaat cgatctgccg ctcgtcgaac ggctcgacct gggtgcgagc
     2161 aagaatgatg acgccaatca aggcatcttc acgcactaac ggtacgccca ataccgtccg
     2221 ctgcttgcct acgcgctgcc actcgaattg atcgaattcg ggatcggcca gcacatccgg
     2281 aacgtggacg gtacgccgtt cgagcacgac gcgtccagtc accgaaccac ggtcaacagc
     2341 caccggacgg tcagcgaggt acctgatgtg atccgcctcg acctggttgc cggcaacga
//
LOCUS       DASUGJ010000668         4621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_108219, whole genome shotgun sequence.
ACCESSION   DASUGJ010000668 DASUGJ010000000
VERSION     DASUGJ010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108219"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFV93_15180"
     CDS             complement(<1..733)
                     /locus_tag="VFV93_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166746.1"
                     /translation="MTLRRWMGLVMLVALVVVTSGKIVSTAANQVPKSNAGEEKVRIH
                     IPIPAETTPQDSTAVDTGDTNGSDSQESTSGAETSNTADSTTGDETDAGDQTADESSE
                     TGDAGQPATTDGAGAPAVQGAVVIPRPTATASSVPTVVPTSTPAPVAPTATSTPAPTA
                     TPAPPVPGNVQSAGVPPGPTSGVSWSVDGCSGSAGATGWAFMPSARAGVLTVAITNGQ
                     PGAILDCVVYLANTGTAPIAISGVVA"
     gene            complement(730..2340)
                     /locus_tag="VFV93_15185"
     CDS             complement(730..2340)
                     /locus_tag="VFV93_15185"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF036452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5166747.1"
                     /translation="MRSSRSRNASVFGFLGAAMLVVVIVFFLPTSLGGRTDYVITSGI
                     SMEPDIHQGDLIILRKTDDYSIGDAVAYRDPDIGRVFHRIIARDRHTFVMQGDNNSWT
                     DNYQPSEGEIVGERWIHLPGVGTWLERVRQPIGMALLATLSGVLLLAPRRVAKRLRRA
                     HRPPTHGRDERPRRSGSAAGILSQDGQMIMSAVGVLALASVLLAAIAYTRPTQHSTTN
                     VVGYDQRMSFSYTAEPRGDVYEGGLVQSGQPIYLALVDDLSLSFDYSITSDLPVATSG
                     NYQLDAIVAFDEGLTRTIPLIGTTAFDGPEVAFSTTLTLSDIQRVIDQIAQQIGANGK
                     HTVSIVPTINLVGMVGDRAVDESFAPALLFELDSVALRPATKAATPDNANATDPFSPT
                     KAGTVEEPATIPATISMLMLDVKVVTARQIAIVGLGLSVLGGLVVAIMLFSALRAAEP
                     HRVKARYGHLLIQVSDSDLGMIGRVVEVISIDDLVRVAEREGRMILHQQHGAVHDYFV
                     QDVDVTYRYWTRDASAPAANAMTEAARG"
     gene            complement(2463..2912)
                     /locus_tag="VFV93_15190"
     CDS             complement(2463..2912)
                     /locus_tag="VFV93_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166748.1"
                     /translation="MRRMIRVSIVLAIMAVLATSAYAFTAANNVPASSAGFGGAEISG
                     YTVSNIHYTLGEGADCESVTLPNDENNYGNCVTQVAFTLAGTNAPTNVSVHFEVPDFG
                     NLPPHQYSVTGNECLISNGASAGTWDVSCNIREFAASIEFLRIAAAQ"
     gene            complement(3584..4237)
                     /locus_tag="VFV93_15195"
     CDS             complement(3584..4237)
                     /locus_tag="VFV93_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166749.1"
                     /translation="MTITALTASAAGLDVDGGVIQVFEPEVNIQLPGEGGASVKITPQ
                     NLNAASNGPPLEAQLRLTGACSQDDIVVSSITLGVAGEHRTIPRTGQVSRNPNKLIVR
                     FDRQAVINLIGDYQGNTVTLVVRGRDATGCEFAGADTIGYKSATKGVDSGAGDGSQSN
                     SDAGGGDEAPPPVEGDGSEPTTDDTTTTDTTTTDQSGTDGTSGSEPSGSEPGPTDAQ"
     gene            complement(4270..>4621)
                     /locus_tag="VFV93_15200"
     CDS             complement(4270..>4621)
                     /locus_tag="VFV93_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166750.1"
                     /translation="GVKPLRIFGGTATLIGLLVSGAIAATVFLGLGRGEAAKVQARFK
                     TVFIEASGMNAPRPASIIDVASLQDLARLAQRDGGIVFHQQEDSTRKRYFVPDGATIY
                     QYLSQPLARDGQAR"
BASE COUNT          843 a         1441 c         1473 g          864 t
ORIGIN      
        1 cggcgacaac gccgctgatc gcgatcgggg ccgtgccggt gttagcgagg tagacgacac
       61 aatccagaat cgcccccggc tgcccgttgg tgatcgcgac cgtcaggacg ccggcccggg
      121 cggagggcat gaaggcccag ccggtcgcac ccgccgaacc actgcaacca tcgacggacc
      181 aactaacgcc cgatgtcggt ccgggcggga cgccggcgct ctggacgttg ccgggcacgg
      241 gtggtgctgg tgtcgcggtc ggtgcaggcg tgctggtggc ggttggagcg accggcgccg
      301 gtgtcgatgt cggcaccacc gtcggaacgg acgacgccgt cgcggtcggc ctggggatga
      361 caacggcacc ctggacggcg ggagcacccg caccatctgt cgtcgcgggt tgcccggcgt
      421 cgccggtctc actcgattcg tccgccgtct ggtctcccgc atcggtttca tcgccggtcg
      481 tgctgtcagc cgtgttggac gtctcggctc cgctggtcga ttcctggctg tcgctgccat
      541 tggtgtcgcc ggtgtcgacg gcggttgagt cctgcggcgt cgtctcggcg gggatcggga
      601 tgtggatccg aaccttctct tcgccggcgt tggacttcgg gacctggttg gctgccgtcg
      661 acacgatctt cccgctggtg acaaccacca gcgccacgag catgacgagc cccatccacc
      721 tgcgcagcgt catcctcggg cggcctccgt catcgcgtta gcggccggcg cgctcgcgtc
      781 acgcgtccag tagcggtagg tgacatcaac atcctggacg aaatagtcgt ggacggcgcc
      841 atgctgctga tggaggatca tccgcccctc gcgttcggca acgcgcacca ggtcgtcgat
      901 gctgatgacc tcgacgacgc gcccgatcat accgaggtcg gagtcgctca cctgtatcag
      961 caggtggccg tagcgtgctt tgacacgatg cggctcggca gcacgcaacg ccgagaacaa
     1021 catgatggcg acgacgagac caccaagcac agacagcccg agaccgacga tcgcgatctg
     1081 gcgcgccgtc acgaccttga catccagcat cagcatcgag atcgtcgctg ggatggtggc
     1141 aggttcctcg acggtgcctg ccttggtcgg gctgaacgga tcggtcgcgt tcgcgttgtc
     1201 cggcgtcgct gctttggtag cgggccgcaa ggcgacagaa tcgagctcaa agaggagggc
     1261 cggcgcaaac gactcatcaa ccgcacggtc gccgaccatg cccaccagat taatggttgg
     1321 aacgatgctg actgtgtgct tgccgttggc gccgatctgc tgcgcgatct gatcgatgac
     1381 ccgctggatg tcgctcaacg tcagtgtcgt cgaaaaggca acctcgggtc cgtcaaatgc
     1441 ggtcgtacca atcagcggga tcgtgcgagt gagtccctca tcaaatgcga cgatggcgtc
     1501 aagctggtag ttgccggacg tcgcgaccgg aagatctgac gtgattgagt aatcgaacga
     1561 gagactcaga tcgtcgacga gcgccagata gatcggctgt ccggactgca ccagaccgcc
     1621 ctcgtagacg tcgccgcgcg gttcggctgt gtaggagaac gacatgcgct ggtcgtatcc
     1681 gacgacgttg gtcgtcgaat gctgcgtcgg tcgtgtatag gcaatcgcgg ccagcagcac
     1741 cgaggcgagc gccaggacac cgaccgccga catgatcatc tggccgtcct ggctgaggat
     1801 gccggccgcc gatccggaac gtcgcggtcg ctcgtctcgc ccgtgagtcg gcggtcgatg
     1861 cgcgcgtcgg aggcgcttcg ccacacggcg cggcgccagc agcagcacgc ctgaaagcgt
     1921 tgccagcagc gccatcccga ttggctggcg tacccgctca agccacgtgc caaccccggg
     1981 aaggtgaatc cagcgctcgc ccacaatctc cccctctgac ggttgatagt tgtcagtcca
     2041 cgagttgttg tcaccctgca tcacaaacgt gtgtcgatcc cgagcgatga tacggtggaa
     2101 cacgcgtccg atatccggat cgcggtatgc gacagcgtca ccaatgctgt agtcgtccgt
     2161 cttgcgcaga atgatgagat ctccttgatg gatgtccggt tccatgctga ttccggatgt
     2221 gatcacatag tcggtccggc cgcccaggct ggtcggcagg aagaagacga tgaccacgac
     2281 cagcatggcc gcacccagga aaccgaagac cgacgcattc cgggatcggg aacttctcac
     2341 cctcgaacgt cctcagcgtt gacgtcaggc tccggcctga ccatcacacc tgacagggat
     2401 cgggagcgga acgacatcgc cccgcccccg gcccgtcaaa ccgacaagct cagaactcga
     2461 cgctactgag cggcggcgat tcgaaggaac tcgatgctgg ctgcgaactc acggatgttg
     2521 cagctgacgt cccacgtgcc cgcgctggcg ccgttggaga tcaagcactc gttaccggtg
     2581 accgagtact ggtgcggcgg caggttgccg aaatcaggaa cctcaaagtg gaccgagacg
     2641 ttggtcggag cgttggtccc ggccagcgtg aacgccacct gggtaacaca gttgccatag
     2701 ttgttctcat cgtttggcaa cgtgaccgac tcgcagtcgg cgccttcacc gagggtgtag
     2761 tgaatgttgg acaccgtata gccactgatc tccgcgccgc cgaagccggc cgacgatgca
     2821 ggaacgttgt tcgcagcggt aaacgcgtag gcgctggtcg caaggacggc catgatggcc
     2881 aagacgatcg agacacggat catgcgtcgc atgaatgggg gtcctttccc tgtcgtccct
     2941 gttcgggacg atccaccgat tcaggtggcc cccgccgttg aagcatcctc gatgcacgca
     3001 gaggacaggc gggcggccgg tgttcgtctg tcgcaagccg gcccgcggga gcttccacct
     3061 ccctgggacc ttggtcatcg acatggcgtg atcaggtcag gcaccacctc ccccgcacga
     3121 gacgccccca cacccccgat gtgctcgcct gcaatagcgc cgggcatgtg tcaaccggcg
     3181 aattcaggca acgggcgcta cgttgttcgc gtggttccac cgtatcacgc caagaattgc
     3241 gccaatacga ccgttgggtg caatgattcg gtgacaatct tgtaagatga atccttgcaa
     3301 cgaggcgtcg cggtgaatgg cgaaattcat ggtgcgttca gagttggctg aacgatcgac
     3361 gccatgttat gtctctcgca tcgagcgttg gattgctgcg gcactttcga ttgtgtcggg
     3421 cggcaacaat gttcgctgga ttcggggacg ggattcggga aacggggttt tcggccaggg
     3481 ctgctgccct cgcctgacag gcagcagccc cgccaaactg cacgcccggc ggggctgata
     3541 caaggaatcg agacgatacg ttcaggggag cgacggacaa gggctattga gcgtcggtcg
     3601 ggcccggctc cgatccgctc ggctcggaac cgctggttcc atccgttccg gactgatctg
     3661 tcgtcgtggt atcggtcgtt gtcgtgtcat cagttgtagg ttcgctccca tcgccctcga
     3721 cagggggcgg tgcttcgtca ccaccgcctg cgtccgaatt ggactgcgaa ccgtccccgg
     3781 caccgctatc gacgcctttc gtcgctgact tgtacccgat tgtgtcggcg cctgcgaact
     3841 cacagccagt cgcatcccgc ccgcgtacaa cgagcgtgac ggtgttgccc tggtagtcgc
     3901 cgatcaggtt gatgacggcc tgccgatcga aacggacgat cagcttgttc ggatttctgg
     3961 aaacctggcc ggttcgcggg atggtccggt gttcgcctgc gaccccgagc gtgatgctgg
     4021 agacgacgat atcgtcctga gagcaggcac cggtaagtcg gagctgggcc tccagcggcg
     4081 ggccattgct ggctgcgttc agattctgcg gggtgatctt gaccgaagcc ccgccttcac
     4141 ccggcaactg aatattcact tccggttcga aaacctggat caccccaccg tcaacatcga
     4201 gtcccgcggc tgacgccgtc agggcagtga tcgtcatgac cagaattacg agtaaggcga
     4261 accgcctgat catcgcgcct gcccatcacg agcgagcggc tgagacaagt attgatagat
     4321 ggttgcgcca tcaggaacga agtagcgctt gcgggtcgag tcctcctgct ggtggaatac
     4381 gatgccgccg tcacgctgag cgagcctcgc gagatcctgc aaagacgcga catcaataat
     4441 cgacgccggc cgcggtgcgt tcatgccgga cgcctcgatg aagacggttt tgaatcgcgc
     4501 ctggactttc gcggcttcgc ctcgccccag tccaagaaac accgttgcgg caatcgctcc
     4561 cgagacgagc agaccgatca gtgttgcagt tccgccgaag attcggagtg gcttgacgcc
     4621 g
//
LOCUS       DASUGJ010000669         8457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1497803, whole genome shotgun sequence.
ACCESSION   DASUGJ010000669 DASUGJ010000000
VERSION     DASUGJ010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497803"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..756)
                     /locus_tag="VFV93_15205"
     CDS             complement(259..756)
                     /locus_tag="VFV93_15205"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX5166751.1"
                     /translation="MPASRAASGMLRARPLLVGIMLLGIIGIGIAGYLTYVHYNEDAL
                     VCTTGGCETVQQSEYSTIGGIPIAIFGLLMFTTVTLLAAARLTDRLPISFETATMLAW
                     TMLLTSLLYYAYLTYIEVFELEAICQWCVSSSIVALLMFVLESILLFRDTSAEAESID
                     PTSNR"
     gene            complement(746..1561)
                     /locus_tag="VFV93_15210"
     CDS             complement(746..1561)
                     /locus_tag="VFV93_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5166752.1"
                     /translation="MSQRRNDKNRSASRKAARASGEASAQSETISRRAQRRIDEERDR
                     KRQRFLMIGGAVVVALIIVGALITFNRSDDGDGASDLAITVPEARDASVPQDGRTLGS
                     PDAPVTIIEYADFQCPFCGDWAKTIEPRLVQDYISTGQVRLEFHAFPFLDDRADGSES
                     DNATEAALCAQDQGQFWAYHDLLFNNQSGENEGAFSRDRLIEMARNVNGLDVDQFTSC
                     IDGDEHEDEVQALYQGAIDAGVNSTPTFDVNGTKVTGINYPQLQQAIEAALAS"
     gene            complement(1706..1837)
                     /locus_tag="VFV93_15215"
     CDS             complement(1706..1837)
                     /locus_tag="VFV93_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166753.1"
                     /translation="MPAFRAIRDDLVADGQTAWMMAARLWDELTMDCLAAEFDAATD"
     gene            1924..3165
                     /locus_tag="VFV93_15220"
     CDS             1924..3165
                     /locus_tag="VFV93_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166754.1"
                     /translation="MRGMIVIALIAAVTMVAGGVIASHIGDDAGGNGPTGSVETATTT
                     PMTGSQPTTSGTQGGAPADQTAQTLAGTTGQQPSGSTDSVSTTPAGTGQIANVLQTKA
                     PVRGAPINGIQRIEDYYSEQYTFDNFTIGWRPGAFPKERADQVAEQARRALDEVNQKL
                     GTNDHGEIEILLADQMYLEEDCHGCQGYAFSDFRQVYILQDGSMAEDEFEALLTHEIG
                     HVIAGSNIALPHVATLFMAEGLATWLMTPALVEQGYIEPRQIAAWALKAGILPPIWQL
                     RKADFEGRTGARLEYDPAASFTQFLIETYGMDDYKRIYAAELPDDVVGKTWEQLEQEW
                     HDWLAEWADNSVGDATATSWWNASALVKQGFDRLYADETTVTQEQYGELATARVVLNK
                     GDVEQAVALMQASGLVPGTAN"
     gene            3321..4091
                     /locus_tag="VFV93_15225"
     CDS             3321..4091
                     /locus_tag="VFV93_15225"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX5166755.1"
                     /translation="MVTSIDTDAYLARIGYSGPRSATIETLRDVHRAHLLAVPFENLD
                     IHLGREIVLDETRLVTKIVGERRGGFCYELNGAFAALLRSLGFSVRLLSAGVRRDDGT
                     FGPEFDHMLLHVDLDEPWLADVSTFMEPIRLSDDMQNDSRDVYQLDRSGEQFTLLRQE
                     PDNTWSARFRFSLTPWHLSDFAGMSHYHQTSPDSDFTQKRICTRLTPDGRVSLTDGRL
                     IATRDGLRTETPIANEAQFADALLEHFGIILSSHERGS"
     gene            4159..5520
                     /locus_tag="VFV93_15230"
     CDS             4159..5520
                     /locus_tag="VFV93_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX5166756.1"
                     /translation="MANGTHTKYLLDESRMPAAWYNLVADLPVPPPPVLHPGTGQPIG
                     PADLAPLFPMEIIRQEVTGEQYVEIPEAIQAVYRLWRPTPLYRAHRLEAHLQTPAKIF
                     YKYEGTSPAGSHKPNTAVAQAYYNKQEGVTRLTTETGAGQWGSALAMACAFFGIELKV
                     YMVRVSYEQKPYRRALMEAWGARCIASPSVETQAGLAVLEHDPESNGSLGIAISEAVE
                     DAAGRDDTKYSLGSVLNHVLMHQTIIGQEAQMQMEDAGAYPDVIYGCAGGGSNFAGLT
                     FPFVKDKLAGRDVRIVAVEPSACPTLTRGIYAYDFGDTGMMTPLVKMHTLGHSFIPAG
                     IHAGGLRYHGMAPLVSLLKEQGLIDAIAYPQNPVFDAAITFGRTEGIVPAPESAHAIM
                     ATIQEALRCRESGEAKTILFGLSGHGNFDLSAYQQYLAGNLTDYDLPETEIERALAEL
                     PAV"
     gene            complement(5573..6154)
                     /locus_tag="VFV93_15235"
     CDS             complement(5573..6154)
                     /locus_tag="VFV93_15235"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5166757.1"
                     /translation="MATVTATPTTTGPTPYKFTAEAYHRLAEVGILKPDDRVELIEGQ
                     IIVMAPVGDPHVNAVDRLNEVFVIAVAGKARVSVQNPVRLSQRSEPEPDLLILRRESF
                     GRAGRTEDVLALVEVSDTTLRYDRDTKLPLYARHAIPHVLNVDMTGGAIVHYFEPEDG
                     HYRQRTRYVRGDTFTLEILPSVSVSINVDDLFT"
     gene            complement(6178..6783)
                     /locus_tag="VFV93_15240"
     CDS             complement(6178..6783)
                     /locus_tag="VFV93_15240"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5166758.1"
                     /translation="MLPDTSSRPTLSKAGATMAVELIHYKLTADEYQRMLDTGILNED
                     SPVELIEGELVWMAAHGGQHIRCVTLLAQSLTVAFVGTFYVSTQNSIRLDLRSEPEPD
                     IAILRSLPEGQTPPPTEDVMLVAEVSDSSLMYDLNVKAPLYARAGIPEYWVVDLTARR
                     VLRHSAPRDGRYKVIEEFADTDQVVSMTEPSITIAVSDIFA"
     gene            complement(6809..7639)
                     /locus_tag="VFV93_15245"
     CDS             complement(6809..7639)
                     /locus_tag="VFV93_15245"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX5166759.1"
                     /translation="MRANRLRELLNDGKPSLGTRLHNIWPGVTELVGNAGMFDYVEFV
                     AEYAPYDLHGLDNLGRAIAIFPHMTGMMKIEQQPRTYLAVRALGAGIQNLLFADCRTA
                     ADVEECVRSVRAETPETGGIVGCGLRRDAGYVIDVATPAYVQALEDAVVAIMIEKRGA
                     VENLDSILAVKGVDMVQFGPGDYSMSIGAPGQFTHPRVREAEKYVIETALAAGVQPRA
                     EISAPEEAERYLEMGVRHFNIGSDMSILYRWFLTNGKAMSDLLGTSANAGESAQEYPA
                     "
     gene            7819..8184
                     /locus_tag="VFV93_15250"
     CDS             7819..8184
                     /locus_tag="VFV93_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5166760.1"
                     /translation="MMPLTWIGNSLQTMVYGSLVLDITTEQASRNGDAVALQQSDWAI
                     LNQLAAANNTFVRGSDLLESIWGDDMRDDALFLRSWVQRLNDRLSCGSDATIIDTLLG
                     GYRLVPPDEWTSVPAQFAG"
     gene            complement(8181..>8457)
                     /locus_tag="VFV93_15255"
     CDS             complement(8181..>8457)
                     /locus_tag="VFV93_15255"
                     /inference="COORDINATES: protein motif:HMM:NF015656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166761.1"
                     /translation="ARSTGDLLFSRLAELTPRQAFTAPAEVVPVAQAAGRVSAESITP
                     YPPGIPLVAPGERLTPDVIDYLRAGIEEGMYVSGLADASFATVRVVR"
BASE COUNT         1622 a         2680 c         2661 g         1494 t
ORIGIN      
        1 tcgcccagca acgcggcgtc ggaatcggga tggggacgat gttctacgcc aacggcggcg
       61 gcgagaacaa ggtccgcctg tcctactcct tcaacgacga cgaccagatc cgcgaaggca
      121 tcggaattct ggctgacgtc atccgcgggg cgtaggtaac gtaacgcacc ccaaccggta
      181 ggggcgaggc actcactcgc ccggcttccc gctcgaaccg attggcgggg acacgaaggg
      241 cgagtcagtg cctcgcccct accggttgga ggttgggtcg attgactcag cttccgctga
      301 agtatcccga aacagcagga tcgactccag cacgaacatc agcagcgcga cgatcgacga
      361 ggacacacac cactggcaga ttgcctcaag ctcgaagacc tcgatgtagg tgaggtaggc
      421 atagtagagc aggctggtca gcagcatcgt ccaggccagc atcgtcgccg tctcgaaaga
      481 gatcggcagt cgatcggtaa gccgggctgc ggccagtaac gtgaccgtcg tgaacatcag
      541 cagaccgaag atggcgatcg ggatgccacc aatcgtgctg tattcactct gctggactgt
      601 ctcgcagccg ccagtcgtgc agaccagcgc gtcctcgttg tagtggacgt aggtgagata
      661 gccggcgatc ccgatcccga tgatcccgag cagcatgatg ccgaccagaa gcgggcgagc
      721 gcgcagcatg ccggacgcgg ctcgactagc tggcaagtgc cgcctcgatc gcctgctgca
      781 actgcgggta gttgataccc gtcactttgg ttccgttgac atcgaacgtc ggcgtcgagt
      841 taacgcccgc gtcgatcgcg ccctgataga gtgcctgaac ctcgtcctcg tgctcatcgc
      901 catcgatgca ggacgtgaac tgatcgacat cgaggccgtt gacgttgcgg gccatctcaa
      961 tcagccggtc acgggagaac gcgccctcgt tctctccgga ctgattgttg aacaggagat
     1021 cgtgataggc ccagaactgc ccctgatcct gcgcgcacag ggcggcctcg gtagcgttat
     1081 ccgactcgct cccgtcggcg cgatcgtcca ggaacgggaa ggcgtggaac tccagcctga
     1141 cctgtccggt cgagatgtaa tcctgtacca ggcggggctc gatcgtcttg gcccagtcgc
     1201 cgcagaaggg gcactggaaa tcggcgtact cgatgatcgt gaccggtgcg tccgggctgc
     1261 cgagtgtccg gccatcctgc ggtactgagg catcacgggc ttccggaacg gtgatcgcga
     1321 ggtctgaggc gccatctcca tcatctgagc ggttgaaggt gatcagcgcg ccgacgatga
     1381 tgagcgcgac gacgacggcg ccgccgatca tcagaaagcg ctggcgctta cggtcgcgtt
     1441 cttcgtcgat ccgtcgttgc gcgcgccggc tgatcgtctc ggattgcgcc gaggcttctc
     1501 cgctcgcacg tgccgcctta cgcgaggcag atcgattctt gtcattccgg cgttgagaca
     1561 cagcttccgg ctccctgttc agacatgaag ggcagatgac caaccacggt cgatccgatc
     1621 cccagccgga tcgacactgg ccaattgtac tgaggcagga ttgacggtgc gccacgaacg
     1681 aatcgtctga tgtgaaaaaa gcgtgtcaat ccgtcgcggc gtcgaactcg gcggcgaggc
     1741 aatccatcgt gagctcgtcc cagaggcggg cggccatcat ccaggccgtc tgcccatcgg
     1801 caactaaatc gtcgcggatc gcccggaagg ctggcatcca gtggccggcc tatggtacac
     1861 ccgttcggca cactaggttg ccgcacggtc acagcctgcc aggtctgaga ggagtcccgc
     1921 cgcgtgcgcg gaatgattgt tattgcgctg atcgccgccg tgacgatggt tgccggcggc
     1981 gttatcgcct cgcacatcgg agacgacgcc ggaggaaatg gtccgaccgg cagcgtcgaa
     2041 acagccacca cgacaccgat gaccggctcg caaccgacca cctcaggcac gcagggcgga
     2101 gcacctgccg atcagacagc gcagaccctg gccggcacga caggccagca gccatccggc
     2161 agcactgaca gtgtatcaac caccccagcc gggaccgggc aaatcgccaa tgtcttgcag
     2221 acgaaagcgc cggttcgcgg cgcgccgatc aatggcattc agcgtatcga agactactac
     2281 tcggagcagt acacgttcga caacttcacg atcggctggc ggcccggcgc gttcccgaaa
     2341 gagcgtgccg accaggtcgc agagcaggca cgtcgcgcgc tcgacgaggt caaccagaag
     2401 ctggggacga acgaccacgg cgagatcgag atcctgctgg ccgaccagat gtacctggag
     2461 gaggactgcc acggttgcca ggggtacgcc ttctccgact tccgtcaggt ctacatcctg
     2521 caggacggca gcatggccga ggacgagttc gaggcactac tgacacacga gatcggccac
     2581 gtcatcgccg gtagcaacat cgcgctgccg cacgtcgcga ccctcttcat ggccgagggg
     2641 ctggcgacct ggctgatgac gccggcgctg gtcgagcagg gctatatcga gccgcgccag
     2701 atcgcggcct gggcgctgaa ggccggcatc ctgccgccga tctggcaact gcgtaaggcg
     2761 gatttcgagg gccgaaccgg cgcacggctg gaatacgacc cggccgcttc attcacgcag
     2821 ttcctgatcg agacctacgg gatggacgac tacaagcgca tctacgccgc cgagctacca
     2881 gacgacgtcg tcggcaagac ctgggagcaa ctggagcagg agtggcacga ctggctggcc
     2941 gagtgggccg acaacagcgt cggagacgcg actgcaacat cctggtggaa cgcctcggct
     3001 ctggtcaagc agggcttcga ccggctctac gccgacgaga cgaccgtcac ccaggagcaa
     3061 tacggcgagc tcgcgaccgc ccgcgtcgtg ctcaacaagg gcgatgtcga acaggccgtc
     3121 gcgttgatgc aggccagcgg gctggtaccc ggcacggcga actgatgcat tgcgagtgct
     3181 gagacctgac tgctgagtgc tggctggtga atctacatca ttgtttcgat tgctgtgacc
     3241 tcacgcctcg gtatggttga agcctgtcgg ccgcgacggt gctgtcgatc cgggcacgca
     3301 acgagagcgg ggcaagattc atggtcacat cgatcgatac tgacgcctat ctggcgcgga
     3361 ttggctacag cgggccccgg tcggcgacga tcgagacgtt gcgcgatgtg caccgggcac
     3421 acctgctggc cgtgccattc gagaatctgg atatccacct cggccgcgag atcgtgctgg
     3481 acgagacacg actggtcacg aagattgtcg gcgagcggcg cggcgggttc tgctacgagc
     3541 tcaatggcgc gttcgcggcg ctgctacgca gcctgggatt ctcggtgagg cttctctcag
     3601 ccggtgtccg tcgcgacgat ggcacgtttg ggccggaatt cgaccacatg ctgctgcacg
     3661 tcgatctgga cgagccgtgg ctggccgatg tcagcacctt catggaacca atccgtctca
     3721 gcgacgacat gcagaatgac agccgcgacg tctatcaact ggatcggagc ggtgagcagt
     3781 ttacgctact acggcaggag ccggacaata cctggtccgc tcgctttcga ttctcgctga
     3841 ctccgtggca cctgagcgac ttcgccggta tgtcccacta ccaccagact tcgcccgatt
     3901 cagactttac tcaaaagcgc atctgcacgc gcctgacacc ggacggccgg gtctcattga
     3961 ccgatggcag gttgatcgcc acccgcgacg gcctgcgaac cgagacgccg atcgctaacg
     4021 aagctcaatt cgcggacgcc ctgctggaac acttcgggat catcctgtcc agtcacgaac
     4081 gcggctcctg acggccgttc cattcgctac aatcactcgt ccgaggaagc gatccagtca
     4141 gcggagggag cggtaccgat ggcgaacggg acacatacca agtatctgct cgatgaaagc
     4201 cggatgccgg cggcctggta caacctggtt gccgacctac ccgttccgcc accgccggtg
     4261 ctgcaccccg gcacgggcca gccgatcggg ccagccgacc tcgcgccact cttcccgatg
     4321 gagatcattc gccaggaagt gaccggcgag cagtatgttg agattcccga agcgatccag
     4381 gccgtctacc ggctctggcg accgacaccg ctttaccgcg cccatcggct ggaagcgcac
     4441 ctccagacac cggcgaagat cttctacaag tacgagggca ccagcccggc cggtagccac
     4501 aagccgaaca cggccgtcgc ccaggcgtat tacaacaagc aggagggcgt gacgcggctg
     4561 acgaccgaga cgggagccgg ccagtggggc agcgcgctag cgatggcctg tgccttcttc
     4621 gggatcgagc tcaaggtcta catggtgcgc gtctcctacg agcagaagcc ctaccggcga
     4681 gccttgatgg aggcatgggg tgcccgctgc atcgccagcc cctcggtcga gacgcaggcc
     4741 gggctggccg tcctggagca cgacccggag tcgaacggca gcctcgggat cgccatctcc
     4801 gaagccgtcg aggacgccgc cggccgcgac gacacgaagt actcgctcgg ctcggtcctc
     4861 aaccatgtcc tgatgcacca gacgatcatc ggccaggaag cgcagatgca gatggaggac
     4921 gccggcgcct acccggatgt catctacggc tgcgccggtg gtggcagcaa cttcgccggg
     4981 ttgaccttcc cgttcgtcaa ggacaagctg gccggtaggg atgtgcggat cgtcgctgtc
     5041 gagccgtcgg cctgcccaac gctgacacgc ggcatctacg cctacgactt cggcgacacc
     5101 ggcatgatga caccgctggt caagatgcat acgctcggcc actcgttcat cccggccggc
     5161 atccacgccg gaggcctgcg ctaccacggg atggcgccgc tggtgagcct gctcaaggag
     5221 caggggctga tcgacgcgat cgcctacccg cagaacccgg tcttcgacgc cgcgatcacc
     5281 ttcggccgga cggaagggat cgtcccggcg ccggaatcgg cccacgcgat catggcgacg
     5341 atccaggagg cgctgcgttg ccgcgaatcg ggcgaggcga agacgatcct cttcggcctc
     5401 tccggccacg gcaacttcga cctgtccgcc taccagcagt acctggccgg caacctgacc
     5461 gactacgacc tgcccgagac ggagatcgag cgcgcgctgg cagagctgcc ggcagtgtga
     5521 cgtgaccagg acagaacgtt atcgtgcgac acgtgatcga acatgtacgt cactacgtga
     5581 acaggtcatc gacattgatt gagaccgaaa ccgacggcag gatctcgagc gtgaatgtat
     5641 cgccccggac gtatcgcgta cgttgacgat agtggccatc ttccggctcg aaatagtgga
     5701 cgatggcgcc acccgtcatg tcaacgttca gcacgtgcgg gatcgcgtgc cgtgcataca
     5761 gcggcagctt cgtgtcccgg tcgtagcgta gggtcgtgtc cgacacttct accagcgcca
     5821 gtacatcttc cgtccgaccg gccctgccga agctctcacg ccgcaggatc agcagatccg
     5881 gctccggctc cgagcgctga ctcagccgaa ccggattctg gacgctcacg cgtgccttcc
     5941 cggcgacggc gataacgaac acttcgttca gccgatcgac ggcattgaca tgtggatcac
     6001 cgacaggcgc catgacgatg atctgtcctt cgatcagctc aacgcgatcg tccggtttca
     6061 gaatgccaac ctcggccagc cggtggtatg cctcagcggt gaacttataa ggggtggggc
     6121 cggtcgttgt cggagtggcg gtgacggttg ccatgctcgc tcctctactg gtctgggtca
     6181 ggcgaagatg tcggaaacgg cgatcgtgat cgatggttca gtcatcgaca caacctgatc
     6241 cgtgtctgcg aactcttcaa tgaccttgta gcggccgtca cgcggcgcgc tatgccggag
     6301 gacgcgccgc gcggtgagat cgacgaccca gtactccggg atgcccgccc gcgcgtagag
     6361 cggagccttc acgttgaggt cgtacatcag cgacgaatca gagacttctg cgacgagcat
     6421 gacatcttca gtcggaggcg gcgtttgccc ttcagggagg gatctaagaa tggcaatgtc
     6481 tggctcaggt tccgaccgta gatcgaggcg gatggagttc tgcgtgctta cgtagaaggt
     6541 accaacgaat gcgacggtta acgactgggc cagcaacgtt acacaccgaa tgtgctgccc
     6601 accatgcgca gccatccaga caagttctcc ctcgatcaac tcgactggtg aatcttcgtt
     6661 caggataccc gtatcgagca ttcgctgata ttcatccgca gtgagtttgt agtggatcaa
     6721 ttcgactgcc atggttgccc ccgctttcga aagggtcggt ctgctgctgg tatctggaag
     6781 catacataat ccactgcaca gctcggggct acgccgggta ctcctgcgct gactcaccgg
     6841 cgttcgcgga cgtgccgagc aggtcgctca tcgccttgcc gttggtgagg aaccagcggt
     6901 agaggatcga catgtcgctg ccgatattga agtggcgcac gcccatctcc aggtagcgtt
     6961 ccgcctcctc gggcgcgctg atctcggccc gtggctggac gccggccgcc agcgccgtct
     7021 cgataacgta cttctcggcc tcgcggacgc gcgggtgggt gaactgaccg ggagcgccga
     7081 tgctcatcga gtagtcgccg gggccgaact ggaccatgtc gacgcccttg acggccagga
     7141 tgctgtcgag gttctcgacg gcgccgcgct tctcgatcat gatcgcgacg accgcatctt
     7201 ccagagcctg gacgtaggcc ggggtggcaa catcgatgac gtagccggca tcacgccgca
     7261 ggccgcagcc aacgatgccg cctgtctccg gcgtttcggc gcggaccgag cgaacgcact
     7321 cctcgacatc ggcagccgtc cggcagtcgg cgaagagcag gttctggatg ccggcgccga
     7381 gcgcacgaac ggcgaggtag gtgcgcggct gctgctcgat cttcatcatg ccggtcatgt
     7441 gcgggaagat cgcgatcgcc cggccgaggt tgtcgaggcc gtgcagatcg taaggtgcgt
     7501 actcggcaac gaactcgacg tagtcgaaca tgccggcgtt gccgaccagt tcggtcactc
     7561 ccggccagat gttgtgtagc cgcgttccga gcgacggctt accgtcgttg agcagttccc
     7621 gtagtcgatt cgcgcgcatg ctgcttctcc cactctcgtt aaccccaatt cggccggggg
     7681 catcctagca tggagcgccc gtcacaattg tgttacagac cgccgcaacc ccctttcgat
     7741 ctgtgaaatt tctcactagg atgtggatgg agatgggccg cgacagccgc gacgctgcgg
     7801 atggatggga agcgttcgat gatgccgctg acttggatcg gaaactcctt gcaaacgatg
     7861 gtctacggtt cgctcgtcct cgatatcacg accgaacagg catcccggaa tggggatgca
     7921 gtcgcgctac agcaatcaga ctgggcaatc ctcaatcagc ttgcggcggc gaacaacacc
     7981 ttcgttcgcg gatcggatct gctggaatcg atctggggcg acgacatgcg tgatgatgcg
     8041 ctgtttctcc gctcctgggt tcagcgactg aacgaccggc tcagttgcgg ttccgacgcg
     8101 acgatcatcg acacccttct cggcggctac cggttggtcc cgccggatga gtggacctcc
     8161 gtccccgccc agttcgcggg ctaacgcacg acgcgaacgg tcgcgaacga cgcatccgcc
     8221 aggccggaga cgtacattcc ctcctcgatt cctgcgcgca gatagtcgat gacatccggc
     8281 gtcaagcgct ctccgggcgc gacgagcgga atgccgggcg ggtagggtgt gatgctctcg
     8341 gcactcaccc ggccggctgc ctgcgcgacc gggacaacct cagcgggcgc cgtgaacgcc
     8401 tgtcgcgggg tcagctccgc cagccggcta aatagcagat cgccagtcga tcgagcc
//
LOCUS       DASUGJ010000670         3327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1082458, whole genome shotgun sequence.
ACCESSION   DASUGJ010000670 DASUGJ010000000
VERSION     DASUGJ010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082458"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2183)
                     /locus_tag="VFV93_15260"
     CDS             complement(<1..2183)
                     /locus_tag="VFV93_15260"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5166762.1"
                     /translation="MRSKSRQFRSILVATIVLSIVAQFAIPVRARTGGDISGADRSSV
                     VTPQADAVTPVTLDLDVVASGFMQPTDLVEPHDGTGRLFVVERLGLVRVIKSGQVLDE
                     PFLDLTGMVSNGTWEQGLLGIAFHPDFAHNGIFFVTSSPAPDEWRLERYVVSSDPNRA
                     DPASAQTVLSFDHPGGNHYGGKIVFGDDGYLWISVGDGGGGYDQYGNAQNLDSLLGKI
                     LRIDVDRGDPYAIPADNPFVGTSGARPEIWAYGLRNPWRFSFDRLTGDLYIGDVGETT
                     WEEVNFEPAGSPGGRNYGWPQMEGMSCSQVAPDCDPSLYVLPISQHGRDDGCAVTGGY
                     VYRGDETSPLWGTYVFADYCNGTIWTAERSGDGWVSGIQLRTPLNISAFGESDDGSIY
                     VVDFTRGGIYQLMSTEDAPAPKIIRTGPGSLLAGGVSCGLNVRGWNFAEGARVRIADE
                     ARETRYIDSTWLQVRLTSDEIAQPALLSVVVDNGVGTQSAQSTLPINDRGGSNGAIYE
                     RWAHDDLPVLDGSASRTWLWGSSGFSCPMAEEYAEGPNGQRMVQYFDKSRMEITQPEG
                     DPSSTWYITNGLLATELMTGNIQLGDTLFEQREPAEINVAGDPDDPSGPTYRTFNSVS
                     DHAPLTVGTTLTQRINRDGRVWVDGTFAARRVTAAYLDTVTNHTIASPFWDYMHLSGL
                     VFIDGKYLTSPIFENPYYATGRPLTEAYWTSVEVGNVKHDVLAQC"
     gene            complement(2298..>3327)
                     /locus_tag="VFV93_15265"
     CDS             complement(2298..>3327)
                     /locus_tag="VFV93_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166763.1"
                     /translation="PASNFAFGWNVGLRGDEGGAEHNQRTADLVKDSGFGWVRFQIEW
                     QSFERQPEQWDPLPTDRKIDELSSQGLKILICVAKAPEWALSAEDGQFLRDYEEFGRF
                     MAFVGNRYKGKVQAWEIWNEQNLAIEVNGYVSVGEYVKLIKAGYTSLKAVDPDAMVLF
                     GGLTPNGVNDPTIAVDDAQYLRDAYNYNGGEIANYFDILGVHLNGTHNSPDNTWPDNV
                     GENEGWNDHPSFFYRRAEELRDIMISVGDSAKPMWITEVGWSTANMAPGREYGVNNTE
                     EEVAQYLTRALDIAINEWDFVTGAFIWNLNWATLVEPEHHMYPSSAIREDWSPRPAYD
                     AMKAFPKS"
BASE COUNT          570 a         1078 c         1043 g          636 t
ORIGIN      
        1 aagcactggg cgaggacatc gtgcttcacg ttgccgacct caaccgaggt ccagtacgcc
       61 tccgtcagcg ggcgtccggt cgcgtagtac gggttctcga agatcgggga tgtcagatac
      121 ttcccgtcga tgaagacgag accggacaag tgcatgtagt cccagaacgg cgacgcaatc
      181 gtgtggttgg tgaccgtatc caggtaggcc gccgtcacgc ggcgcgccgc gaacgtgccg
      241 tcgacccaga cgcgtccgtc gcggttgata cgctgcgtca gcgtcgtccc gaccgttagc
      301 ggcgcatgat cgctgacgct gttgaaggtt cggtaggtcg ggccactggg gtcgtccgga
      361 tcgccggcga cattgatctc tgccggttct cgctgctcga acaatgtgtc accgagctgg
      421 atgttgccgg tcatcaactc cgtcgccagc agaccgttgg tgatgtacca cgttgatgag
      481 ggatcgccct ccggctgggt gatctccatc cgcgacttgt cgaagtactg aaccatccgc
      541 tggccattcg ggccttcggc atactcctcc gccatcgggc agctgaatcc cgacgatccc
      601 cagagccacg tgcgacttgc agagccatcc aggaccggca ggtcgtcgtg tgcccagcgc
      661 tcgtagatcg cgccgttgct gccgccccgg tcgttgatcg gcagcgtgga ctgggcggac
      721 tgcgttccga cgccgttgtc gacgaccacc gacaacagag ccggttgggc aatctcgtcg
      781 gaggtcaggc gcacctgtag ccaggtgctg tcgatgtagc gggtctcgcg cgcttcgtca
      841 gcgatacgca cccgagcgcc ctcagcgaag ttccaaccac gcacgttcag accgcaactt
      901 actccacctg ccagcaggct gccggggccg gttcggatga tcttcggagc cggcgcgtct
      961 tccgtggaca tcaactgata gatgccgcca cgagtgaagt cgaccacgta gatcgagccg
     1021 tcgtcgctct caccgaaggc gctgatgttc aatggcgttc gcagttggat gccggacacc
     1081 cagccgtcgc ccgaccgctc ggcggtccag atcgtcccgt tgcagtagtc tgcgaagacg
     1141 tacgtgcccc agagcggcga tgtttcgtca ccccgataga cgtagccgcc cgtcacggcg
     1201 cagccgtcgt cgcggccatg ctggctgatc ggcaggacgt agagcgaggg gtcacagtcg
     1261 ggagcgacct ggctgcagct catcccctcc atctgcggcc agccgtagtt gcgcccgcct
     1321 gggctgccgg ccggctcgaa gttgacttcc tcccaggtgg tctcgccgac atcgccgatg
     1381 tagagatcac cggttagccg gtcgaacgag aagcgccacg ggttgcgcag cccataggcc
     1441 cagatctcgg gacgggcgcc gctggtgccg acgaacgggt tgtcggccgg tatcgcgtag
     1501 ggatcgccac gatcgacatc gatccgcagg atcttgccca gcagcgagtc gaggttctgg
     1561 gcattgccgt actggtcgta gcctccgccg ccatcgccga cgctgatcca caggtagccg
     1621 tcatcgccga agacgatctt gccaccgtag tggttgccac ccggatggtc gaacgacagt
     1681 accgtctgcg cgctggccgg atcagcccga ttgggatcgg aggagacgac atagcgttcc
     1741 agccgccact cgtcgggcgc cggtgaggat gtgacaaaga agataccgtt atgggcaaag
     1801 tctgggtgga aggcgatccc cagcaatccc tgctcccagg tgccgttcga aaccatgccg
     1861 gtcaggtcca ggaacggctc gtccagcacc tgaccgctct tgatgacacg gacgagtccg
     1921 agccgctcga caacgaacag ccgtccggtt ccgtcgtgcg gctcgacaag atcagtcggt
     1981 tgcatgaacc cgctggcgac gacatccagg tcgagcgtga cgggcgtgac agcatcagcc
     2041 tgtggcgtca cgaccgacga ccgatccgcg ccggagatgt caccacccgt tcgcgcccgg
     2101 accggtattg cgaactgggc cacgatcgac aatacgatcg tagcgaccag aatcgaccgg
     2161 aattgacggc tcttcgatct catgcgcgct tctccacccc ccgcgagttg cgttctcgcc
     2221 tttcacctgt tcggcggaca tgcgacctca gacccgcttc gtggcgagcc gccaggtgct
     2281 gcacgtcatc cggaaactca gctcttcgga aacgccttca tcgcatcgta tgccggtcgc
     2341 ggcgaccagt cctcgcggat ggcggacgac gggtacatgt gatgctccgg ttcgaccagc
     2401 gtggcccagt tcaggttcca gatgaacgcg ccagtaacga agtcccactc gttgatcgcg
     2461 atatccagcg cgcgggtcag gtactgggcg acttcctctt cggtgttgtt gacgccatac
     2521 tcgcggcccg gagccatgtt cgcggttgac cagccgactt ccgtgatcca catcggcttg
     2581 gccgagtccc caacgctgat catgatgtcg cgcagctcct ctgcacgccg gtagaagaag
     2641 ctggggtggt cgttccagcc ctcgttctca ccaacgttat ccggccaggt gttgtcgggc
     2701 gagttgtggg tgccgttgag gtggacaccg aggatgtcga agtagttggc gatctcgccg
     2761 ccgttgtagt tgtaggcgtc gcgcaggtac tgcgcgtcgt cgacggcgat cgtcgggtcg
     2821 ttgacgccgt tgggcgtcaa gccgccgaac agcaccatcg cgtcgggatc gacggccttc
     2881 aggctggtgt agccggcctt gatcagcttg acgtattcac cgacgctgac gtagccgttg
     2941 acttcgatcg cgaggttctg ctcgttccag atctcccatg cctgcacctt acccttgtag
     3001 cggttgccga cgaaggccat gaagcgcccg aactcttcgt agtctcgcag gaactggccg
     3061 tcctcggcgc tgagcgccca ttccggggcc ttcgcgacgc agatcaggat cttgagtccc
     3121 tgcgagctca gctcgtcgat cttgcgatcg gtcggcaggg gatcccactg ctccggctgg
     3181 cgctcgaacg actgccactc gatctggaaa cgcacccagc cgaagccact gtccttgacg
     3241 aggtcggccg tccgctggtt gtgctctgcc ccgccctcgt cgccacgcag gccgacgttc
     3301 cagccgaacg cgaagttcga ggcaggc
//
LOCUS       DASUGJ010000671         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1639807, whole genome shotgun sequence.
ACCESSION   DASUGJ010000671 DASUGJ010000000
VERSION     DASUGJ010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639807"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VFV93_15270"
     CDS             complement(<1..851)
                     /locus_tag="VFV93_15270"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5166764.1"
                     /translation="MRGLVAELREKTAAVSERGAAGDERSIARHRERGKLPVRERIDK
                     LLDPGAAFLELSPLAADGMYDGDAPGAGIVTGIGKVSGTDCVIVANDATVKGGTYYPM
                     TVKKHLRAQEIALQNRLPCIYLVDSGGAFLPLQDDVFPDREHFGRIFYNQAQMSSKGI
                     AQVALVMGSSTAGGAYVPAMSDETVIVRGTGTIFLGGPPLVKAATGEDVTAEELGGAS
                     VHARVSGVADHEALDDEHALAIGREIIASLAWKKPDLPWERLPSRPPAVDPTDLYSVI
                     SADLRKG"
     gene            complement(1024..2841)
                     /locus_tag="VFV93_15275"
     CDS             complement(1024..2841)
                     /locus_tag="VFV93_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166765.1"
                     /translation="MLKATYEQTRKSKQQMGWRRFVAGLRLSTILPTLASAAVVLVLA
                     ALAVGLIVNRPGLIGLGPTALPAPTSTHTPTPDPWAAFIGTFVADPGDFDNGLQTMTV
                     VRSDTSTLDIVVTDTIASVCELRPSTMTGIGHVQEQARLVIPTPDYRCDDGTTPHLIN
                     GDPTPLNDALRNLTYRLDWTTGNLIAGQTEVWVRQPDSTQAPTESPSPTLAPTAPPSA
                     TPVATLAPTPGTTPEVSLEPGPVVVSTLPRQDGEFITFDLQGAGSDWNLAAQDPATGN
                     VRVIVDTTDLVDCADSDTCANIIRKAEWSADGRWVAFEVSNADLGENAIGPCAPALGL
                     WVRDPNGALTQLSTPCDSPPAPADRPVRELWEWSPVANELAYVRVDGSDDDLFVIDAT
                     TGARTLVATGEMRLPYPVESDSIAWSPDGSRIAYVEGNAVYAVEVATGQRSLLSDSFD
                     QIIDLAWSPDGTQIFVQDQERNRIQVMNADGSDLHALVEGTDACCETAWSPNGDRIVY
                     MLSLVDGEGWHSEVWTNTPDGLNPIQVASLSGCAVATDSLPVWANETQVAYLGCDGWV
                     VENADGTGDPQPTDQLHWRSWYGGGLVGGDLALLGEANR"
BASE COUNT          470 a          944 c          969 g          534 t
ORIGIN      
        1 tagcccttgc gcagatcggc cgaaatgacc gagtagaggt cagtcgggtc gacggccggt
       61 ggtcgactgg gcaggcgttc ccacggaagg tcgggtttct tccacgccag gctggcgatg
      121 atctcgcgtc cgatagccag cgcgtgctcg tcatccagcg cttcgtgatc tgccacgccg
      181 gagacgcggg cgtggacgct ggcgccgcct agctcctccg ccgtgacgtc ctcaccggtc
      241 gcggccttaa cgaggggagg gccgcccaag aagatcgtgc ccgtgccgcg aacgatgacc
      301 gtctcgtcgc tcatcgccgg cacgtaggcg cccccggcgg tgctgctgcc catcacgagc
      361 gcgacctggg cgatgccttt ggacgacatc tgggcctggt tgtagaagat gcggccgaag
      421 tgctccctgt ccgggaagac gtcgtcctgg agcggcagga acgcgccacc actgtcgacc
      481 agatagatgc acggcagccg attttggagc gcgatctcct gggcgcggag gtgcttcttg
      541 acggtcatcg ggtagtaggt gccgcccttg accgtagcgt cattggcgac gatcacgcag
      601 tccgtgccgc tgaccttgcc gatgccggtg acgatgcccg cgcccggggc atcgccgtcg
      661 tacatgccgt ctgccgcgag tgggctcagc tcgagaaacg cggcacccgg gtcgagcagc
      721 ttgtcgattc gttcgcggac gggcagcttg ccgcgctcac ggtgccgggc gatggagcgt
      781 tcgtcgcccg ccgcgccgcg ctcactgacc gctgctgtct tctcgcgcaa ctccgccacg
      841 agcccgcgca tggctgcgct gttcgcgcga gcctcatcgc tatttgggtc gacccgcgtc
      901 gtcagtaccg ccacgccgcg atgttacggg cacaaaagag cgccggcctt tcgacaccgc
      961 gccactcttg cggaggcttc cgtggaagtt aaggcccggc cggctgtccg gcgattgact
     1021 gcgtcagcgg ttggcctcgc cgagtagcgc gaggtcgccg cccaccaggc cgcccccgta
     1081 ccagctccgc caatgcagct ggtcagtcgg ttgcgggtcg ccagtgccat ccgcgttctc
     1141 aactacccag ccgtcgcatc cgagataggc gacctgtgtt tcgttagccc acacgggaag
     1201 cgaatcggtc gccaccgcgc agccgctgag gctcgcgacc tgaatcgggt tcaaaccgtc
     1261 cggcgtgttc gtccacacct ctgaatgcca gccttcgccg tcgacaagcg aaagcatgta
     1321 gacgatgcga tcgccgttgg gtgaccacgc cgtctcgcag caagcgtcgg ttccctcgac
     1381 caaggcatgc agatccgatc cgtcggcgtt catcacttgg atccgattgc gctcttggtc
     1441 ctggacgaaa atctgcgtac cgtcgggcga ccaagccagg tcgatgatct ggtcgaacga
     1501 gtcagacaac agggaccgct gcccagtcgc cacttcgacg gcgtacaccg cgttgccctc
     1561 cacgtacgca atccgggaac cgtccggcga ccaggcaatg gagtcggact ccacggggta
     1621 cgggagacgc atctctccag tggcgacaag cgtccgagca ccggtcgttg cgtcgatcac
     1681 gaagaggtcg tcgtccgagc catcgacacg gacgtatgcg agttcattgg caacgggcga
     1741 ccactcccag agctcacgaa ctggccggtc agcaggggcc ggcggtgagt cgcacggcgt
     1801 cgacagctgt gtgagcgcgc cgtttggatc gcgtacccag agcccgagcg cgggcgcgca
     1861 cggaccgatc gcgttctccc cgagatctgc gttgctgacc tcgaacgcga cccagcggcc
     1921 gtcggcggac cattcggcct ttctgatgat gttcgcgcag gtgtcggagt ccgcacagtc
     1981 gaccaggtcc gtcgtgtcca cgatgacgcg gacattgccc gtcgccggat cttgtgccgc
     2041 caggttccaa tcggacccgg ccccctgaag atcgaaggtg atgaactctc catcctgacg
     2101 cggcagcgtt gagacgacga ccggaccagg ctccaggctc acttcaggcg tcgttcctgg
     2161 cgtgggcgcc agagtcgcaa ccggagtcgc gctcggaggt gccgtaggcg caagggttgg
     2221 gctcggcgac tctgttggcg cctgggtgct gtccggctgg cgtacccaca cctcggtctg
     2281 gccggcgatc aggttgccgg tggtccaatc gagtctgtat gtcaggttgc gcagcgcgtc
     2341 gttgagcggc gtcggatcgc cattgatcag gtggggcgtg gtgccgtcgt cgcagcggta
     2401 atcgggcgtc gggattacca accgggcctg ctcctggaca tgaccaatgc cggtcatggt
     2461 cgacggtctg agttcgcaga ccgatgcgat cgtgtcagtg acgacgatgt cgagtgtgct
     2521 ggtgtctgag cggacgacgg tcatcgtctg gagcccgtta tcgaagtcgc ctgggtcggc
     2581 cacgaacgtt ccaataaacg cggcccatgg atcgggcgtg ggcgtatgtg tcgatgtcgg
     2641 cgctggtaaa gccgttgggc caagtccgat caggcccggt cgattgacga tcaggccgac
     2701 ggccagggct gccaatacaa gcacgactgc cgcgctcgcc agggttggga ggatcgtcga
     2761 cagccgtaga cccgcgacga agcgacgcca gcccatctgc tgcttgctct tgcgcgtctg
     2821 ctcgtaggtt gccttcagca agtggtcggg gaagtggcgc actgcgtcct cctcgagcca
     2881 gagcgagatc cggtcttcta cgttctgtgg ctcagtc
//
LOCUS       DASUGJ010000672         5231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_388177, whole genome shotgun sequence.
ACCESSION   DASUGJ010000672 DASUGJ010000000
VERSION     DASUGJ010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388177"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFV93_15280"
     CDS             <1..216
                     /locus_tag="VFV93_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5166766.1"
                     /translation="LPRMLILSAAIGAFSGVGGLYLSYYRDVASGAAVVLVATAIFFV
                     VWTATNLAQPIRRASRRHAITQTSDAV"
     gene            358..732
                     /locus_tag="VFV93_15285"
     CDS             358..732
                     /locus_tag="VFV93_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5166767.1"
                     /translation="MSAQTATNTDLELLAQLNDDYIQSVQNGDVARFDEILAPEFYCS
                     NPDGTLIDRAAFLRQTAQPVTISDLQAHDVLIRLFGDVAIIHARTTYTLPSGATATGR
                     YTDCWARIDGRWLAVSAHVTRG"
     gene            756..1238
                     /locus_tag="VFV93_15290"
     CDS             756..1238
                     /locus_tag="VFV93_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5166768.1"
                     /translation="MSPRSDRLVTEFTAVNDDLISCVSECSDEQWQRQSVEEGWTVAA
                     VAHHVAVSNGAIAGMLSSQVAGNPDLPNISTADLNANNAAHARDYATVGKQETLDLLR
                     SNGDAVVQTLQSIDDDTFFDRPAGIFGGNELSVGQVAQFVLVGHTAGHTASIKASLAD
                     "
     gene            1353..1835
                     /locus_tag="VFV93_15295"
     CDS             1353..1835
                     /locus_tag="VFV93_15295"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5166769.1"
                     /translation="MSARIDQYIAQFQAASRDLIALVTDCSEEDWQRVSPGEGWTVAA
                     IAHHVALRNGVIAGMIDNVLAGSADVPHISRADLLTENATHAEEYAAVSKEEVLALLR
                     DNGDAFVRTLGRIEDDAILDRAGEVFAGTDLCVDYQIEEVVIAHAITHAASIRATLAA
                     "
     gene            complement(1915..3345)
                     /locus_tag="VFV93_15300"
     CDS             complement(1915..3345)
                     /locus_tag="VFV93_15300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5166770.1"
                     /translation="MAGEETAIGYLNENYKRFSKLARDIWEHPEVGLEETFASKAIAD
                     ELDKAGFAVEFGAGGMETAFVASWGEGSPIIGILGEYDALPSLSQDATPERNELVPGG
                     PGHGCGHNLYGVGALGAALAMQEAMKEQGITGTVRYYGCPAEETLTGKTYMARDGVFD
                     DLDASITWHPGYANTVPINSSSLAMNSFKVHFHGVASHAAAVPHMGRSALDGAMLMDI
                     GVNYLREHIIQDARIHSVIKDGGEAPNVVPPTATIWYYVRAPKRDQVESIYQRMLDCA
                     KGAALMSGTTYEVEFLTGCYEMLPNATVSNIMLDKMEQLGGIEFSHDDMEFARKLQES
                     ISPEIIEMSREQTLKNSKAGTTADDIGDVLCENVIRPSDEFHIGHGSTEVADVSQITP
                     TANMWACCHPLGSPGHSWQITASSGAEIGFKGMDFAAKSMALTGLELMVNKEAVETAR
                     REFTEATGGRKYVTPLPEGATPQKGG"
     gene            complement(4865..>5231)
                     /locus_tag="VFV93_15305"
     CDS             complement(4865..>5231)
                     /locus_tag="VFV93_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166771.1"
                     /translation="RREAVMRMANAANDARDIEQLNRLLEIWSRPQDTTDIRDLGTLR
                     ARVAERDVKHDELGRQLEALQNNALGKMIRRPKAELTKYIKREEERLRRELATLRLKR
                     RRLLTAVEDRRRELAEASD"
BASE COUNT          993 a         1613 c         1585 g         1040 t
ORIGIN      
        1 ctgcccagga tgctgatcct ctcggccgcg atcggtgcct tctccggcgt cggtggcctc
       61 tacctttcct attatcgtga cgtcgcctcc ggcgccgccg ttgtcctcgt cgcgaccgcc
      121 atcttctttg tcgtctggac agccacgaac ctcgcccaac ccatccgacg tgcttcgcgc
      181 cgccacgcga tcacccagac tagcgacgcc gtctgacatc ggcgggctct cggtgtcgtc
      241 ccgaggcgac gagggattgg gtgccggttt gcgagcccaa ctggacctgt ctgattcgtc
      301 gggagcgtcg tatcctgccc agaccaacgc tgtccgaacg cgcgaaaggg gaattgcgtg
      361 tccgcccaga ccgccaccaa tactgacctc gaactgttgg cgcaactcaa tgacgactac
      421 atccagtcgg tccagaacgg cgatgtcgcg cgtttcgatg agatcctggc cccggagttc
      481 tattgcagca atccggatgg caccttgatc gatcgtgcag cgttcctgcg tcagacggcg
      541 cagccggtca cgatctccga cctccaggcg cacgatgttc tgatccggct atttggtgac
      601 gtcgccatca tccacgcgcg cacgacttac acactcccca gcggtgcgac ggccaccggt
      661 cgatatactg actgctgggc gcgcatcgac ggccgctggc tggctgtctc cgcacatgtc
      721 acccgcggtt gaacgatttt gactcaggag gccggatgtc accacgaagc gatcgactcg
      781 taaccgaatt cacggctgtc aacgacgatc tgatctcatg cgtttccgaa tgcagtgacg
      841 agcagtggca gcgacagagc gtggaggaag gctggaccgt cgcagccgtt gcgcatcacg
      901 tcgcggtctc caatggtgcg atcgccggaa tgctcagcag ccaggttgcc ggaaatcccg
      961 atctgccaaa catttcgacg gcggatctca acgcgaacaa tgccgcgcac gcgcgggact
     1021 acgcgaccgt cggaaagcag gagacgctcg acctgttacg gtcaaacgga gatgcggttg
     1081 tgcagacgct ccagagcatc gatgacgaca cgttcttcga tcgaccggcc ggcatctttg
     1141 gcggcaacga gctcagcgtc ggacaggtcg cccaattcgt tctcgtcggc cacacggccg
     1201 gccacaccgc gagtatcaaa gcgtcgctgg ctgattgagt cgtccgcatc cccggccctt
     1261 ccgacaggcg tagaccgggg agcgactcat ccaggccctt gaccacggcg cgtcgctcac
     1321 gctcgaccca atccaggatg ggaggatgtg tcatgtccgc acgcatcgat cagtacatcg
     1381 cgcaattcca ggcagccagc cgtgatctca tcgcgctcgt caccgactgc agcgaagagg
     1441 attggcaacg cgtcagtccc ggcgaaggct ggacggttgc cgcgatcgca catcatgtcg
     1501 cattgagaaa cggcgtcatc gccgggatga tcgacaacgt gctggctgga agtgccgatg
     1561 ttccacacat ctcacgtgcg gacctcttga cagagaacgc gacccacgcg gaggagtatg
     1621 cggccgtctc gaaggaagag gtgcttgcgt tgctccgcga caatggcgac gccttcgtcc
     1681 gaaccctcgg gcggattgag gacgatgcga ttctcgatag agcaggcgaa gtgttcgccg
     1741 gcaccgatct ctgcgttgat taccagatcg aagaggtcgt gatcgcccac gcgatcaccc
     1801 acgccgccag catccgcgcg acgctggcgg cgtgacgctc aatacattct gacccctctc
     1861 ccaggcatgg gagaggggtc agctcgaagc gtcgcgcaga cgggaagggg ccgatcagcc
     1921 gcccttctgc ggggtcgcgc cttccggaag cggcgtcacg tacttgcggc caccggtggc
     1981 ttcggtgaac tctcgccggg cggtctcgac cgcctccttg ttgaccatca gctccagacc
     2041 ggtcagcgcc atcgacttgg cggcgaagtc catgcccttg aagccgatct ccgcgccgga
     2101 cgaggccgtg atctgccagc tgtggcccgg cgagccgagc gggtggcagc aggcccacat
     2161 gttggcggtc ggtgtgatct ggctgacatc ggccacctcg gtcgaaccgt gcccaatgtg
     2221 gaactcgtcc gatgggcgga tgacgttctc gcagagcaca tcgccgatgt cgtcggccgt
     2281 cgtgcctgcc ttcgaattct tcagcgtctg ctcgcggctc atctcgatga tctcaggaga
     2341 gatcgactcc tgcagcttgc gggcgaactc catgtcgtcg tggctgaact cgatcccgcc
     2401 cagctgttcc atcttgtcca gcatgatgtt gctgacggtc gcgttcggca gcatctcata
     2461 gcagccggtc aggaactcga cctcgtaggt cgtgccagac atcagcgccg cgcccttggc
     2521 acagtcgagc atgcgctggt agatcgactc gacctggtcc cgcttcggcg cgcgcacgta
     2581 ataccagatc gtcgccgtcg gcgggacaac gttcggcgct tcgccgccgt ccttgataac
     2641 gctgtggatg cgggcgtcct ggatgatgtg ctcgcgcagg tagttcacgc caatgtccat
     2701 cagcatggcg ccgtcgagcg cgctgcgccc catgtgcggg acggctgccg cgtgggacgc
     2761 gaccccgtgg aagtggactt tgaacgagtt catcgccagg ctggagctgt tgatcggcac
     2821 cgtgttcgcg tagcccggat gccaggtgat cgacgcgtcc agatcgtcga agacgccgtc
     2881 acgggccatg taggtcttgc cggtcagcgt ctcctcggcc gggcagccgt agtagcggac
     2941 cgtgccggtg atgccctgct ctttcattgc ctcctgcatc gccagcgccg caccgagcgc
     3001 gccgacgccg tacaggttgt ggccgcagcc gtggcccggc ccgcccggca ccagctcgtt
     3061 gcgctccggc gtcgcgtcct gcgacaggct cggcagtgcg tcgtactcgc cgaggatgcc
     3121 gatgatcggg ctaccttcac cccagctggc gacgaaggcc gtctccatcc cgcctgcgcc
     3181 gaactcgacg gcgaagcccg ccttgtcaag ctcatcagcg atcgccttcg aggcgaacgt
     3241 ctcctccagc ccgacttccg gatgctccca gatatcgcgc gccagcttcg agaagcgctt
     3301 gtagttctcg ttcagatacc cgatcgccgt ttcttcgcca gccataacct tccctccctg
     3361 ctacgcacga gccacgcact cccgcgcagt tcgcctccgg ccacgtgtcg gcgcatggta
     3421 gcaggaatct cgaagattcg tatcaattgg ggtgcacaat gccgggagtt agtcgctggc
     3481 gtgggttagc tgacggcgac ttctgccctt cagcacagca ggttcgggtt atagtgagag
     3541 caaacgataa catcgagcca agattgggac atcagatggg gttcgacgca gtagcgtttg
     3601 gtagattctt gcgcaatttg cgcggagcgc ggtcgcaagg tgaggttgcg agggactctg
     3661 gagtgcctca acatcgaatt tcagcatttg agcgaggtga ggtacaagat ccaggcatcc
     3721 taacgttgct gcggctcact tcatactatg gactcgctcc caatcaggta gcgattgaaa
     3781 tgggtgtttg ggcgacagga cacaccgaac cagatcagtt ccccgccacg atggcgaacc
     3841 atcacacggt gtcgaacggt gggtcaacag gcggtgataa gtcccaaccg gggaactggt
     3901 cagtatttca acggggaaat caaaggtggt gcgcggagcg ccgtcacccg agcgggaagc
     3961 gggaatatct ttacggtcaa agtgaagcgg aagttcgatc caagcttcga gagaatgatc
     4021 caggtgatcg tcgcgagaac gatacgctgg cgacgtatct ggatcaatgg atcgtgcgat
     4081 ttgccgaaag taagccgcga gagtccaagg tcgcccgtta tcgcgtggcg ataagagacc
     4141 acatcatccc gctactgggc aatcggttgc tgtcacagct gacgggactt gagattgcga
     4201 atctcatcag cgaggtacag aactcctcaa gtataggcaa cgctcgactc gttcgaacgg
     4261 tgttgcacac cgcacttgag accgcagcta atactggaat cattcaaacg aatccggctc
     4321 taccaatccg cagtaagagg tctcatccca gcgaatcaca agtttctaga cgagtgaacc
     4381 aagagtcaca gcctgctgct gcagataggc gacggcgacc cctcatttgg tatctagggt
     4441 caatactaga actctgtcca ggcgagatac aaagcctgcg ctggcgtgac attgatttag
     4501 atgaggaaac tctgcatgtc cagtctacat cacgacggaa tcgtagccag caagagtcga
     4561 ctgcctcgga gggtctactg actagtcggg tggtgcagat cccctctcag gtgatagagg
     4621 cgctgctcca gcatttcgaa gatgaatgtc taaaaggtcg tgggatgcca gatgaccatc
     4681 tattttatag cgactctggt gcgagactgg ctgttaatca atttcgacgt atctacaaca
     4741 agttaatgaa ttgacactgc tgtcgagaat tcaacacggg agccgtgcgc ttcgcgtatg
     4801 caagcacagt actgtagggc catgaacaaa gttttccgat gctgctcaca acgacacgta
     4861 aggttcagtc gctcgcttcg gccagctcgc gacggcggtc ctcgacggcg gtgagtagcc
     4921 ggcggcgttt gaggcggagg gtcgccagtt cgcgccggag gcgttcttcc tcgcgcttga
     4981 tgtatttggt cagctccgcc ttcggccggc ggatcatctt gccgagcgcg ttgttctgta
     5041 gcgcctcaag ctgacggccc agctcgtcgt gcttgacgtc ccgctcggcg acccgcgcgc
     5101 ggagcgtgcc gaggtcgcgg atgtcggtcg tgtcctgtgg cctggaccag atctccagca
     5161 gccggttgag ctgctcgatg tcgcgcgcgt cgttggcggc gttcgccatc cgcatgacgg
     5221 cttcgcggcg g
//
LOCUS       DASUGJ010000673         5236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1083060, whole genome shotgun sequence.
ACCESSION   DASUGJ010000673 DASUGJ010000000
VERSION     DASUGJ010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083060"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VFV93_15310"
     CDS             <1..1054
                     /locus_tag="VFV93_15310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX5166772.1"
                     /translation="VQASSGTRGKLTVVSYTAHDVEIWAEVCARSLALGGTRPGDVVQ
                     NAYGYGLFTGGLGIHYGAERMGATIIPMSGGNTIRQVMILKDLRAKTLTCTPSYALII
                     GEIMASEGVVPADLDLRFGILGAEPWTEEMRLEIERRLGIHAVDIYGLSEVIGPGVAC
                     ECVEARDGLHVQEDHFLIEVVNPNTGETLPDGHYGELVFTTLTKEAMPVIRYRTGDIA
                     AIISEPCSCGRTTRRMSRLRGRFDDMIILHGVNVYPSEIEAALLEFDEVAPFYQIILD
                     WNGRTETIEVQAEMTPRYAEETSGDDRSVLSQQITRILKSRFDLTMQVSLFAPGELGR
                     LEVGKAVRVVDRRRGG"
     gene            complement(1074..2444)
                     /locus_tag="VFV93_15315"
     CDS             complement(1074..2444)
                     /locus_tag="VFV93_15315"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX5166773.1"
                     /translation="MTAPILIASRLRLDPPPSERAAEGQLLRQQTPRGAHAVWTPARS
                     RPSIIDLLEASNQGRLRSLVPIRHGRMLASPFAFLRGSASIMAHDLVNTPVTGILAQI
                     CGDAHLGNFGGFATPERNLIFDLNDFDETLRGPWEWDVKRLAASVFVCGRVNGLSDEQ
                     CRDAVMAAARSYRHRTRRYAEMRYLDIWYERIDADQAVDHGDSGLEPLEKALAKGRRK
                     TNVETLPKLAEQVDGTWLIGNDPPLLTHQAAALTRRLPGLVASYKESLPADRRVVLER
                     YRLVDLARKVVGIGSVGTRCYVALLFGSHDDDPLFLQIKEAGRSVIEQALGERQHGQH
                     GERVVSGQRIMQAASDLFLGWAQDGRRHYYVRQLRDMKSSVDLNDLDAGELIDYASLC
                     GWALAHAHACSGDAAQIGGYLGKSHCFDEAIATFASVYADQTERDFNDLVTAVKHGQI
                     RAEMGV"
     gene            2670..2984
                     /locus_tag="VFV93_15320"
     CDS             2670..2984
                     /locus_tag="VFV93_15320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEX5166774.1"
                     /translation="MAAKVIAMYKRPADEAAFNARYFTAHLDLVRKIPGLQQIEVTRG
                     LGKEAPYWLITEMTYADRDALMAATGSPESAAARADVQEFAADIITVVMVDELETWEP
                     GT"
     gene            3008..3805
                     /locus_tag="VFV93_15325"
     CDS             3008..3805
                     /locus_tag="VFV93_15325"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5166775.1"
                     /translation="MTTSDVLVAERDGGVLMLTMDRPDSLNAFNRELLVGLREQFEQA
                     AGDDAVRCIVLTGAGRGFSSGADLADLREMYGKPEPPSLGPYVRDLYAPLVLAIRRIE
                     KPVIAAINGVAAGAGASIALACDLRIASDQASLIEAFVRIGLIPDTAGTIILPLLVGL
                     AKASELAFTGSRVGADEALRLGLVNRVVPADDLVPQTLAWAHELAALPTRAIGLTKRG
                     FNRALLPELEAILEHEAQLMEEAGRTHDHQEGVMAFLEKRKPEFTGT"
     gene            3906..4175
                     /locus_tag="VFV93_15330"
     CDS             3906..4175
                     /locus_tag="VFV93_15330"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5166776.1"
                     /translation="MTDQDLSDVAGKPVVTYDDEQLGMAEGVVTEAGGGRRFLVVAGT
                     LYGTGKYYVPEEEIRRVGEHRILLGTTRDDLRELDWFAPPDGIAP"
     gene            complement(4172..>5236)
                     /locus_tag="VFV93_15335"
     CDS             complement(4172..>5236)
                     /locus_tag="VFV93_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166777.1"
                     /translation="LATVDLALEFDLAAEVLALFGPSGAGKSMSLKMIAGIETPDRGI
                     IRSGERVLFDSATGIDLPPQRRRAGYVPQQYALFPHLTALENVAYPLRKGLRWPTERA
                     NRRARELLESFGLGELLDVYPGGLSGGQQQRVALARALSSDPEILLLDEPFAALDGPI
                     RAELRNELRALQQRLRVPAVFVTHDLEEAATLASRIAVMLDGSLRQIGSTRDVLDRPV
                     DRQVAELVQARNIISGTIEQTEGVTHVTTSLGRLAIGKRAFADRAAVDLIIRPEVIRI
                     VRENRPLDRLRNQILLDGSVTQIVDHGTRVVIYAAIRATMLEISLSPSAAARLDIEIG
                     GPIRIAIAETDIHIVPALGS"
BASE COUNT          863 a         1691 c         1819 g          863 t
ORIGIN      
        1 cgtccaggca tcgtcgggga cgcgcggcaa gctgaccgtt gtctcctata ccgcccacga
       61 cgtcgagatc tgggccgagg tttgcgcgcg ctcgctggcg ctgggtggca cgcggccagg
      121 cgatgtcgtc cagaacgcct acggctacgg cctcttcacc ggcggcctcg gcatccacta
      181 cggcgccgag cggatgggcg cgaccatcat cccgatgtcc ggtggcaaca cgatccggca
      241 ggtgatgatc ctcaaggacc tgcgcgcgaa gacgctgacc tgcacgccct cgtacgcgct
      301 gatcatcggc gagatcatgg ccagcgaggg cgtcgtcccg gccgatctcg atctgcgctt
      361 cggcatcctc ggcgccgagc cgtggaccga ggagatgcgg ctggagatcg agcgccggct
      421 cggcattcac gccgtcgaca tctacggcct gagcgaggtg atcggccccg gcgtcgcctg
      481 cgagtgcgtc gaggcgcgcg acggtttgca tgtacaggag gaccacttcc tgatcgaggt
      541 cgtcaacccc aataccggcg agacgctgcc ggacggccac tacggcgaac tggtcttcac
      601 gacgctgacc aaggaggcga tgccggtcat tcgctaccgg accggcgaca tcgccgccat
      661 catcagcgaa ccgtgcagtt gcggccggac gacgcggcgg atgtcgcgcc tgcggggccg
      721 cttcgacgac atgatcatcc tgcacggcgt taacgtctac ccctccgaaa tcgaagcggc
      781 cttgctggag ttcgacgagg tcgcgccgtt ctaccagatc atcctcgact ggaacggccg
      841 gaccgagacg atcgaggtcc aggccgagat gacgccgcgc tatgccgagg aaacgagcgg
      901 cgacgaccgg tctgtcctgt cccagcagat cacgcggatt ctcaagagcc gcttcgacct
      961 gacgatgcag gtcagcctgt tcgcgcctgg cgagttgggc cggctggagg ttggtaaggc
     1021 cgtccgggtg gtcgaccgcc gtcgtggcgg gtgaccggcg cgttcgcgtt cggtcagacg
     1081 cccatctcgg cccggatctg cccatgcttc acggcggtga cgaggtcgtt gaagtcgcgt
     1141 tcggtctgat cggcatagac gctcgcgaag gtcgcaatcg cctcgtcgaa gcagtggctc
     1201 ttgccgaggt agccgccgat ctgggccgcg tcgccggagc aggcgtgcgc gtgggccagc
     1261 gcccagccgc agagcgacgc gtagtcgatc agctcgccgg cgtcgaggtc gttgagatcg
     1321 acgctggact tcatgtcgcg taactggcgg acatagtagt gccgtcgccc gtcctgcgcc
     1381 cagccgagaa acaggtcgct ggctgcctgc atgatgcgtt gccccgaaac gacccgctcg
     1441 ccgtgctgtc cgtgctgccg ctcgccgagc gcctgctcga tgacggagcg tcctgcctcc
     1501 ttgatctgca ggaagagcgg gtcgtcatcg tgcgagccga acagaagcgc gacgtagcag
     1561 cgcgtgccga cgctgccaat cccaacgacc ttgcgcgcta gatcgaccag ccggtagcgt
     1621 tcgagaacga cgcggcgatc ggccggcaac gactccttgt agctcgccac cagccccggc
     1681 aggcgacgcg taagcgccgc cgcctgatgc gtcagcaggg gaggatcgtt gccgatcagc
     1741 caggtcccat caacctgctc cgcgagcttc ggcagggtct caacattcgt cttgcggcgg
     1801 cccttggcga gcgccttctc aagcggctcc aggcccgagt ctccgtgatc gacggcctga
     1861 tcggcgtcga tccgctcata ccagatgtcc aggtagcgca tctcggcgta gcgtctcgtt
     1921 cgatggcggt agctgcgcgc ggccgccatt accgcgtctc ggcactgctc gtctgacagg
     1981 ccgttgacgc gtccgcagac gaagacgctc gccgccagcc gcttgacgtc ccactcccac
     2041 gggccgcgca gcgtctcgtc gaagtcgttg aggtcgaaga tcaggttgcg ctcgggcgtg
     2101 gcgaatcccc cgaagttgcc cagatgggcg tcgccgcaga tctgcgccag aataccggtg
     2161 acgggcgtgt tgaccagatc gtgggccatg atcgacgcag agccgcgcag gaaggcgaac
     2221 ggcgacgcca gcatccggcc atgccggatc gggacaagcg accgcaatcg cccctgattc
     2281 gacgcttcga gcagatcgat gatactcggc cgcgagcgcg caggcgtcca gacggcgtga
     2341 gcgccgcgcg gcgtctgctg tcgcaacaac tgtccctcgg cggcgcgctc gctgggcggg
     2401 ggatcgagcc ggagccgtga cgcgatcagg atcggggccg tcatgacgtg ggatgtccct
     2461 ctctggaacg tgggttgaac ggctggacgc tgcaagtggc cccttgacgg cacgcggcgg
     2521 ctgacgcgca tgtgtccggg ccatggtgaa gcaagcgcga tgccagccgt gtttcaggcg
     2581 aacccggctt gaccctacgt gccctgtgcg ggaaagtaat caacgataga cccggcagat
     2641 gtcgcagaca gagatgtcag gaggcacact tggcagccaa ggttatcgcg atgtacaagc
     2701 ggcctgccga cgaggcggcc ttcaatgccc gctattttac cgcacacctc gacctggtca
     2761 ggaagattcc cggcttgcag cagatcgagg tcacgcgagg gttgggcaag gaggctccgt
     2821 actggctgat caccgagatg acgtatgcgg accgcgacgc gttgatggct gcgaccggct
     2881 cgccggagtc ggcggcggcg agggcagacg ttcaggagtt cgccgccgac atcatcacgg
     2941 tcgtgatggt ggatgagctg gaaacctggg agccgggcac ctgatcgacg accgaaagga
     3001 cacgacgatg accacgtccg atgtgctggt agccgaacgg gacggcggcg tcctgatgct
     3061 gacgatggac cggcccgatt ccctcaacgc cttcaatcgc gagttgctgg ttggcctgcg
     3121 cgagcagttc gagcaggcgg ctggtgacga cgccgtgcgc tgcatcgttc tgaccggcgc
     3181 cggccggggc ttctcgtccg gcgccgatct ggccgatctg cgcgagatgt acggcaagcc
     3241 agagccgccc tcgctcggtc cgtatgtgcg cgacctctat gcgccgctcg tgctggcgat
     3301 ccggcggatc gagaagccgg tcatcgccgc catcaacggc gtcgcggccg gggctggagc
     3361 cagcatcgcg ctggcctgcg acctgcggat tgcttcggat caggccagcc tcattgaggc
     3421 gttcgtccgg atcgggctga tccccgacac ggccgggacg atcatcctgc cgctgctggt
     3481 cgggttggcg aaggcgtcgg agctggcctt caccggtagc cgtgtcggcg ccgacgaggc
     3541 gctgaggctc ggcttggtca accgcgtcgt cccggccgac gatcttgttc cgcagacgct
     3601 cgcctgggcg cacgagctgg cggcgctgcc aacccgcgcc atcggcctca ccaagcgcgg
     3661 cttcaaccgc gcgctgctcc ccgagctgga agcgatcctc gaacacgaag cgcaactgat
     3721 ggaggaagcc ggccgcaccc acgaccacca ggaaggcgtc atggcgtttc ttgagaagcg
     3781 caagccggag tttacgggga cgtaacgaga ttgttgggac gtgggacgcg aagtgaatca
     3841 gtccacgtcc catgtcccac gtcccgcgtc ccgcgtcccg aactctcaca ccgaaaggac
     3901 caccgatgac cgatcaggat ctgagcgatg tcgcggggaa gccggtcgtg acctacgatg
     3961 acgagcagtt ggggatggcc gagggggttg tcaccgaggc gggtggcggg cggcgcttcc
     4021 tggtcgtggc cgggacgctg tacggcaccg gcaagtacta cgtgcccgag gaggagattc
     4081 gccgggttgg tgagcatcgc atcctgctcg ggacgacccg cgacgacctg cgcgagctcg
     4141 actggttcgc accgccggac ggtatcgcgc cttaacttcc gagcgccggg acgatgtgga
     4201 tgtcggtttc ggcgatggcg attcggatcg ggccgccgat ctcgatgtcc agtcgggcgg
     4261 ccgccgatgg cgagaggctg atctccagca tggtcgcgcg gatggctgcg tagataacga
     4321 cgcgggtgcc gtggtcgacg atctgggtga cgctgccgtc gagcaggatc tgattgcgga
     4381 gccggtcgag cgggcggttc tcgcggacga tccggatcac ctcgggccgg atgatgaggt
     4441 cgacagcggc gcgatccgcg aaagcgcgct tgccgatcgc cagccgaccc agcgacgtcg
     4501 tgacatgtgt gacgccttca gtctgctcga tcgtgccgga gatgatgttg cgcgcctgga
     4561 cgagttccgc cacctgccgg tcgacgggcc ggtcgagcac atcgcgggtg ctgccgatct
     4621 ggcgtaggct gccgtccagc atgaccgcga tccggctggc aagcgtcgcg gcttcctcaa
     4681 ggtcgtgggt gacgaagacg gccgggaccc tcagccgctg ctggagtgcg cgtagctcgt
     4741 tgcgcagctc ggctcggatc gggccatcga gggcagcgaa gggctcgtcc agcaacagga
     4801 tctccgggtc gctggagagc gccctcgcca gcgcgactcg ttgctgctgg ccgccggaaa
     4861 gtccacccgg gtagacatcc agcagctcgc cgaggccgaa agactccagc aactcgcggg
     4921 cgcgccggtt tgcgcgctcg gtgggccagc gcagcccctt gcgcagcggg taggcgacgt
     4981 tctccagtgc tgtcaagtga ggaaagagcg cgtactgctg tggcacgtag ccggcccggc
     5041 ggcgctgtgg cgggaggtcg atgccggtgg cgctgtcgaa cagcacccgc tcgccggagc
     5101 ggatgatccc gcgatccggc gtctcgatcc cggcgatcat cttcagtgac atgctcttgc
     5161 cggcgccgga ggggccgaag agtgccagca cctcggcggc gagatcgaac tcgagcgcca
     5221 aatcgacggt cgccag
//
LOCUS       DASUGJ010000674         4579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1360650, whole genome shotgun sequence.
ACCESSION   DASUGJ010000674 DASUGJ010000000
VERSION     DASUGJ010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360650"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /gene="gatB"
                     /locus_tag="VFV93_15340"
     CDS             complement(<1..1125)
                     /gene="gatB"
                     /locus_tag="VFV93_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5166778.1"
                     /translation="MEFETIIGLEVHAQVLTNSKMYCGCSSEYFSAPPNTHVCPVCLG
                     LPGVLPVINRVAVDKTIATGLALGSEIPPYNKFDRKNYIYPDLMKGYQISQYDLPMAV
                     GGELEFDVDGERKRVGITRVHLEEDTARLVHRVDADGERSLVDVNRGGVPLMEIVSDP
                     DMRSPQEAREYLIALRRILRYIGASAANMEEGSFRCDANVSQRTTDGKIVGPKVEIKN
                     MNSFRAVERALQYEVERQRAALERGETLIQETRGWVEDQGVTVGQRTKEQAHDYRYFP
                     EPDLPPIELVAVSVDAIRSSMAELPEARLSRLMSAFGLGEAEAALITDEPAIADYYEA
                     AVAAAGGHHRDVANWLVGDLFALARSRGGFNEIRLQPAQLA"
     gene            1519..3897
                     /locus_tag="VFV93_15345"
     CDS             1519..3897
                     /locus_tag="VFV93_15345"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5166779.1"
                     /translation="MVATRSETRLVTLTIDGQQVTVPAGTTILQAAEANGIEVPNLCF
                     QPLLRPWGSCRLCTVEILGSRGGLIESCATPVREGQEISTISQAVVEARQFILQMYLI
                     DHALDCPTCDKSGECYLQDNTYLHNVSANPFRRPKIAMPYKHLRETIDYKWDRCIICS
                     RCTRVCEEMIGVTAITIAGRGLEAEVTTSYGVDLRDTSCTNCGMCIAVCPVGALTDRH
                     FGHHPWELDAVETVCGFCDVGCTLNIEHNRGIVRRVTHLWDRGVNLGYTCERGKWGHE
                     HVQDPLRLRYARVTESGEQHEVELDEALDLAAERLRHYQGAAFAALASPDNTNEDNYA
                     LQQFTRAVMGSNNIDRLISRAQSGVDDALLQSFGVSANPASMQEMRTDSHCALIVGPD
                     IGYYEPIASYWLYWALRYRETRMIVVTSDHQFLSDRSDSWINAAPGTEADVLNAMAKI
                     IIAEGLAQTSAELARLTKAVSTVDIDAVAAATGISTAELRESAIAYATGGKGVHEGVT
                     EYPPSTIWHTVAAGSPNADATALAANNLAIICGHVGKPGGGVLAMRQNANAQGSIDVG
                     CHPAFLPGGLRASDETSRAMFAELWSQRWNEAAVPQNGFKPLRALPSEPGISAGNIAA
                     AIEAGTIKAMYITSQSHQWASPVDDALLAALTKLEFLIVEDCFDSELTRIADVVLPSA
                     MYLEKDGSFTNLDRTVQRVRYAVSSPGDARPSMWFVAEIAGRLGYSLPSDNVSAIMDE
                     ISTVVPAYAGVNFPRLERGGMQWPVSNFGAEQTVYLSVGNGLAPDDIRIVAD"
     gene            3930..4382
                     /locus_tag="VFV93_15350"
     CDS             3930..4382
                     /locus_tag="VFV93_15350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX5166780.1"
                     /translation="MLDEFKGFAVTLRRMTKKPVTFEYPDEKRQFAPRFRGLPSLRAD
                     PETGEALCVACGLCARICPTSCLEMHVVPSEEGDRELGEFILRSGRCLFCGFCAQVCP
                     VDAITMSGAFERSVLERDGLIYTKTELAAFGAQNDTWWGADSPRVTTP"
BASE COUNT          825 a         1591 c         1388 g          775 t
ORIGIN      
        1 ggcaagctgg gctggctgta gccggatctc gttgaacccg ccacgcgagc gcgccaacgc
       61 gaagaggtcg ccgaccagcc agttggcgac atcgcggtga tggccaccgg ccgccgccac
      121 cgccgcctcg taatagtcag cgattgcggg ctcatcggtg atcagcgccg cctcggcctc
      181 tcccagcccg aaggccgaca tcaaccgcga caatcgtgcc tcgggcagct ccgccatcga
      241 tgagcggatc gcatcgaccg agacggctac cagctcgatt ggcggcaggt ccggctccgg
      301 aaagtagcgg tagtcgtggg cctgctcctt ggtgcgctgg ccgacggtga cgccctggtc
      361 ctcgacccag ccgcgcgtct cctggatcag cgtctcgccc cgctcaaggg cggctcgctg
      421 ccgttcgact tcgtactgca gcgcccgctc gacggcccgg aacgagttca tgttcttgat
      481 ctcgaccttc ggaccgacga tcttcccgtc cgtcgtccgc tgcgagacgt tcgcgtcgca
      541 acggaatgat ccctcttcca tattggcggc cgacgcgcca atgtagcgca ggatgcggcg
      601 cagggcgatc aggtactcgc gtgcctcctg tggcgagcgc atgtccgggt cggagacgat
      661 ctccatcagc ggcacgccgc cccgattgac atcgacgagg ctgcgctcac catcggcatc
      721 gacgcggtgg acgagacggg ccgtgtcctc ttcgagatgg acgcgggtaa tgccaacccg
      781 cttgcgctcg ccatcgacgt cgaactccag ctcgccgcca accgccatcg gcaggtcgta
      841 ctgcgagatc tggtagccct tcatcaagtc cgggtagatg tagttcttgc ggtcgaactt
      901 gttataggga gggatctcgc tgccgagcgc cagcccggtc gcgatcgtct tgtcgaccgc
      961 aacgcgattg atgacgggca gcacccccgg caggcccagg cagaccgggc agacgtgcgt
     1021 gttcggcggc gcgctgaaat actccgacga gcagccgcag tacatcttgc tgttggtgag
     1081 cacctgcgcg tgtacttcca ggccgatgat ggtttcgaac tccacgcaac gctctccatc
     1141 aggtattggt gcggcccaaa ttggccgcga gagtatagca cgacgcccgg cgattgttct
     1201 gcaacgccgc cgtgtccagc ctaccactcc atcgtgcaac acgactggtg gcatctaccg
     1261 gtgtcagcac ccgatacgta actgaaaacc gcgtcagatt gtgaaagaac tggcgaagca
     1321 tgctaccatt ccaggcgtcg tcagtgcgtc tgtgacgcgg ctttcgacac tattcccgcc
     1381 attgttgcca gcatcttcac gggatcggac ttcgatacgg gcggccggca cggcttccgg
     1441 acacgagcat cagccccacg cgctcggtca ctccggggca gaactggtca ctgagcaccg
     1501 ggaggcgaat cgccagcgat ggtcgcaaca cgaagcgaaa ccaggctcgt cacactcaca
     1561 atcgacggcc agcaggtcac cgttccggcc ggaacgacga tcctccaggc cgccgaagcc
     1621 aacggcatcg aggtccccaa cctgtgcttc cagccgctgc tccgcccgtg gggctcgtgc
     1681 cgcctctgca ccgtcgagat actcggctca cgcggcggct tgatcgagtc gtgcgccaca
     1741 ccagtccgtg aggggcagga gatctcgacc atctctcagg cagtcgtcga ggcacgccag
     1801 ttcattctcc agatgtacct gatcgaccac gccctcgact gcccgacctg cgacaagtcc
     1861 ggcgaatgct acctgcagga caacacctac ctgcacaacg tcagcgccaa tccgttccgc
     1921 cggccgaaga tcgcgatgcc ctacaagcac ctgcgcgaga cgatcgacta caagtgggac
     1981 cgctgcatca tctgctcgcg ctgcacccgt gtctgcgagg agatgatcgg tgtcaccgcc
     2041 atcacgatcg ccggtcgcgg gcttgaggcg gaggtcacga cctcctacgg cgtcgatttg
     2101 cgcgatacca gttgcaccaa ctgcggcatg tgcatcgccg tctgcccggt cggcgcgctg
     2161 accgaccgcc atttcggaca ccacccgtgg gagctcgatg cggttgagac cgtctgcggc
     2221 ttctgcgatg tcggctgcac cctgaacatc gagcacaacc gcggcatcgt ccgccgtgtg
     2281 acccatctct gggatcgcgg cgtcaacctc ggctacacct gcgagcgggg caagtggggc
     2341 cacgagcacg tccaggaccc gctgcggctg cgctacgcac gcgtcaccga gagcggcgag
     2401 cagcacgaag tcgaacttga cgaagcactc gacctggccg ccgagaggct gcgccactac
     2461 cagggcgctg ccttcgctgc cctcgcctcc cctgacaaca ccaacgagga caactacgcg
     2521 ctccagcagt tcacccgtgc cgtgatgggc tcgaacaaca tcgaccggct gatctcgcga
     2581 gcgcaatccg gcgtcgacga cgcgctcctg cagtcgttcg gtgtctccgc caacccggcc
     2641 agcatgcagg agatgcgcac cgattcgcat tgcgcgctga tcgttggtcc tgacatcggc
     2701 tactacgagc cgatcgcctc ctactggctc tactgggcgc tgcgctaccg cgagacacgc
     2761 atgatcgttg tcaccagcga tcaccagttc ctgagcgacc gcagcgacag ctggattaac
     2821 gccgctcccg gtaccgaggc ggatgtcctc aacgcgatgg cgaagatcat catcgccgag
     2881 ggactggccc agaccagcgc cgagctcgcg cggttgacga aggccgtctc gaccgtcgat
     2941 atcgacgccg ttgcggcagc aaccgggatc agcacggccg agctacgcga atcggccatc
     3001 gcctacgcga ccggcggcaa gggcgtccac gagggcgtta ccgagtaccc gcccagcacg
     3061 atctggcaca cggttgccgc cggctcaccg aacgcagacg cgacggcgct ggctgccaac
     3121 aacctggcaa tcatctgcgg ccacgtcggc aagccgggcg gcggggtgct cgccatgcgg
     3181 cagaacgcca acgcccaggg cagcatcgac gtcggctgcc accccgcctt cctgcccggc
     3241 ggactccgcg cctcggacga gacctcgcgg gcgatgttcg ccgaactctg gtcacagcgc
     3301 tggaacgagg cggccgtccc gcagaacggc ttcaagccgc tccgggcgct gccatccgag
     3361 ccgggcatca gtgccggcaa tatcgcggct gcgatcgaag ccggcacgat caaggcgatg
     3421 tacatcacct cgcagtcaca ccagtgggcc tcgccggtcg atgacgcgtt gctcgcagcg
     3481 ttgacgaagc tggagttcct gatcgttgag gactgcttcg acagcgagtt gaccaggatc
     3541 gccgacgtcg tcctaccgtc agccatgtat ctcgaaaagg acggctcgtt cacgaatctc
     3601 gaccgaaccg tccagcgcgt ccgctatgcc gtctcgtcgc ccggcgacgc ccgcccgtcg
     3661 atgtggttcg tcgccgaaat cgcgggccgg ctgggctact cgctgccgtc cgacaatgtc
     3721 tcggccatca tggacgagat ctcaaccgtc gtccctgcct atgcgggcgt caacttcccg
     3781 cgcctggagc ggggcggcat gcagtggccg gtttccaatt tcggcgccga gcaaaccgtc
     3841 tacctgtcgg tcggcaacgg cctcgcgccg gacgatatcc gaatcgtcgc cgactgatcg
     3901 aaccggcgac ctgaaaggcg attgatccga tgctggacga attcaagggc tttgccgtca
     3961 cgctgcgacg gatgacgaag aagccggtca ccttcgaata tcccgacgag aagcgccagt
     4021 tcgcgccgcg cttccgcggt ctgccgtcgc tgcgggccga tccggaaacc ggcgaagcgc
     4081 tctgcgtcgc ctgcggactc tgcgcccgca tctgcccgac ctcatgcctg gagatgcacg
     4141 tcgtcccatc cgaagagggc gaccgcgagc tgggtgagtt catcctccgc tcgggccgct
     4201 gcctcttctg cggcttctgc gcgcaggtct gcccggtcga cgccattacg atgagcggcg
     4261 ccttcgaacg ctccgtcctc gaacgcgacg gcctcatcta caccaagacc gaactggccg
     4321 ccttcggtgc ccagaacgac acctggtggg gcgccgacag cccgcgggta acaacgccgt
     4381 aaggtcaaat tcaatgacgc aacgcaaggg acgcaacgcg catgcggcgt cccttgtgct
     4441 ttttgacaac atcggtggcc gacgagacca acccccatca atccattccg gtacgggcga
     4501 tatcgcaata tcgccctgtc tccccaccgt ggcgttcggg cgggacacga agcgcgatat
     4561 tgcgatatcg cccgtaccg
//
LOCUS       DASUGJ010000675         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1221863, whole genome shotgun sequence.
ACCESSION   DASUGJ010000675 DASUGJ010000000
VERSION     DASUGJ010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221863"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFV93_15355"
     CDS             <1..264
                     /locus_tag="VFV93_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166781.1"
                     /translation="YQSLVSVEEFLGMVGNFVAIAASLHYAQLYVGDIRIALHGSAIV
                     ERESTHLVDAQPATVGAAQHSGVEQVTEVVHGSAAGASHRANR"
     gene            428..781
                     /locus_tag="VFV93_15360"
     CDS             428..781
                     /locus_tag="VFV93_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166782.1"
                     /translation="MPLQLALLVLIDAGSLVTWRFKAAGATLTTVGAVLLGALAAMEH
                     QPLQGLAVAIAFMVPGVLYWLDWQREQSVRAVTLLAVGFVMLLIVGGIAAFAIHGSLP
                     GSSQFQMLWSNRFLR"
     gene            965..>2496
                     /locus_tag="VFV93_15365"
     CDS             965..>2496
                     /locus_tag="VFV93_15365"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5166783.1"
                     /translation="MRALTRDAHRPPTTLADLFAPLKLSDRMVNQIRWIARIWVIVAA
                     IVFLAFGLSRGIPTNPDLGTWQEPVQLALLVLVSLGSLIAWRWEAIGATVISFGAIFL
                     GVLATVENDPQVSFLAALVFLIPGVLFWFVWQRNRSFKAVVILAVTMLLLLASGGYAS
                     ASVYNYLFGPTHPQSATNAPRIDLVEWVWSGGATPTSITVNTKLADDYDSVRLAVSQS
                     ADMSNAVYSGTDVARDQLNQRVVSFTVDGLTPNTHYFYAIEADGRLDLVRQGQFTSFP
                     SDAASFSFAFASCARTGSNGQVFDAIREAAPLFYMNVGDIHYTYLDTDNPDRFRSQLD
                     EVLTRPAQAALYRSLPIGYVWDDHDYGPNDGDRNSPTRDAARQVYQQYVPHYPLAAGE
                     GDVPIYQAFTAGRVRFILTDTRSERDPDASVDDASKSMLGDAQTEWFKQQLLDARGRY
                     PVIVWVNSGPWIDAAGAGKDTWGGFTTERRELANFIADNQIQGLLMVSGDAHSLAIDD
                     GS"
BASE COUNT          427 a          801 c          755 g          513 t
ORIGIN      
        1 taccagtcct tggtgagtgt cgaggagttc ttggggatgg tcggcaactt cgtcgcaatt
       61 gcggcgtcgc tgcactacgc ccagttgtat gttggcgaca tccgcatcgc gctccatggc
      121 agcgcgatcg tcgagcggga atcgacgcat ctggtcgatg ctcagccggc aaccgtgggc
      181 gccgctcaac actccggagt agaacaagta acggaggtcg tccatggaag cgcagcgggc
      241 gcttcccacc gtgcgaacag gtgaacggcg gcgcccgttt ccactgaacc tgctcgcgtt
      301 ctcagataca gcgtcccaac ggctccgatg gggagcgcga ggctggacgt ccgtccgaac
      361 tccagccttc ctcgccctcg ccgtcagcga gggagtcccc cactccgccg acgaggccat
      421 ctgggagatg ccgctacagc tcgcgctgct ggtactcatc gacgcaggct cgctcgtgac
      481 ctggcggttc aaagcggccg gcgcgacgct caccaccgtc ggcgcagtcc tgctgggagc
      541 gctggccgcg atggagcatc agcctcttca gggtctcgcc gtcgcgatcg cgttcatggt
      601 cccgggcgtt ctctactggc tcgactggca gcgcgagcaa tcggttcgag ccgtcaccct
      661 gctcgccgtc ggcttcgtca tgctgctcat cgtcggtggg attgcagcgt tcgcgattca
      721 tggctcgttg cctggtagtt cgcagttcca gatgctgtgg agcaaccgtt tcctgagata
      781 acggaatggc tccccttcct gtttcggcag gcggctgtag cggaattgtc acacggtcat
      841 cgcgagtgca gcgtgcctgt cacgccgccg gtcatatgct cgacatggcg tcgcccgtgc
      901 ttcgatcggt actcgtgaac caccagacga agcaacgacg catcgacgcc ggaaggaact
      961 tccgatgaga gcccttacac gtgatgcaca tcggcctcca accacactgg ctgatctgtt
     1021 tgctcctctc aagttgtctg accggatggt caaccagatt cgctggattg cgcggatctg
     1081 ggttatcgtc gccgcgatcg tgttcctggc ctttggtctc agccgtggca tccctaccaa
     1141 ccctgatctc gggacgtggc aagagcctgt ccagttggca cttctcgttc tggtgtcgct
     1201 tgggagcctg atcgcctggc gctgggaagc gatcggagcg acggttatct cgttcggggc
     1261 gatcttcctt ggtgtgctcg ccacggtcga gaacgatccc caggtatcgt tcctggccgc
     1321 gctggtcttc ctgattccag gtgttctctt ctggttcgtc tggcagcgca accggtcctt
     1381 caaggcggtc gtgatcctcg cggttacgat gctgctcctc ctggcctctg gcggctacgc
     1441 gtcggcgtct gtctacaact atctgttcgg tccgacccac ccccagtcgg cgacgaacgc
     1501 cccgcggatc gacctcgtcg agtgggtctg gtcgggcggc gcaacgccga cctcaatcac
     1561 cgttaacacg aagctggccg acgactacga tagcgtccgg ttggccgtta gtcagagcgc
     1621 cgacatgtca aacgcggtct attccgggac ggatgtcgcg cgcgatcaac tgaaccagcg
     1681 agtggtctcg ttcaccgttg atggcctgac gccgaatacc cactacttct atgcgatcga
     1741 agcggatggc cgcctggacc tcgttcggca gggacagttc acgagtttcc caagtgacgc
     1801 ggcatcattc agtttcgcct tcgcctcctg cgcgcgaact ggctccaatg gacaggtgtt
     1861 cgacgcgatt cgcgaagccg ctccgctgtt ctacatgaac gtgggtgaca tccactatac
     1921 ctatctcgat accgacaacc cggatcgttt ccgcagtcag ctcgatgaag tgctgacgcg
     1981 accggcccag gcggccctct atcgcagcct gccgatcggc tacgtctggg acgaccacga
     2041 ctacggcccc aacgacggcg accgcaactc accgacccgt gacgcggccc gccaagtcta
     2101 tcagcagtat gtcccacact acccgttggc cgccggtgag ggtgatgtgc cgatctacca
     2161 ggcattcacg gccggtcgag ttcggttcat cctgacggat accagatcgg aacgcgaccc
     2221 ggacgcgtcc gtcgacgatg cttccaagag catgctcggc gatgcgcaga cggagtggtt
     2281 caagcagcaa cttctcgacg caaggggacg ctaccccgtc atcgtctggg tgaactccgg
     2341 accgtggatc gatgcggccg gcgccggcaa ggacacgtgg ggcggcttca cgaccgagcg
     2401 tcgcgaactg gccaatttca ttgcggacaa ccagattcag ggcttgctga tggtcagcgg
     2461 cgatgcgcac tcacttgcga tcgacgacgg atcgaa
//
LOCUS       DASUGJ010000676         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_388453, whole genome shotgun sequence.
ACCESSION   DASUGJ010000676 DASUGJ010000000
VERSION     DASUGJ010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388453"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..881)
                     /locus_tag="VFV93_15370"
     CDS             complement(33..881)
                     /locus_tag="VFV93_15370"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166784.1"
                     /translation="MEVGAGIRRYEVVEGWPKLPEGMRWGQVVQVVVDSQDRVFFFQR
                     IAPGVSIFDKSGEYLGAWPEGGRFTDIHSAYVGKDGEGEYLLIVDRDRNAIARTTLDG
                     EVVWERQEDRYNRPTDAAVASNGDIYVTDGYGNALVHPLSSTGEHIRTWGGPGVAPNE
                     FRLPHGVWMANWNGQETVYVCDRENSRIQRFSLDGEYRGTVPGVRRPTDIVVDADGVR
                     YVSELSSRVTILDEDDHAITRIGGQELREPGQFVAPHSLWQDSEGSLYVSEVLEGQRV
                     QKFRRV"
     gene            complement(987..>2022)
                     /locus_tag="VFV93_15375"
     CDS             complement(987..>2022)
                     /locus_tag="VFV93_15375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5166785.1"
                     /translation="TTIWMLAPDGDGGPEKLVGGDLGARGTAGYGWEAAGIANVLDRS
                     GERFAFNWSDATHPVELWTGVVGGEARQLTHFNEELTSRKIGRTELVRWQSDELEIEG
                     LLIYPVGYQEGTRYPTILHIHGGPSWAWDDHFYCSWHDWGQFLAGQGYAVLMPNPRGS
                     TGRGWEFQIANYADWMGGDYLDSQRGLDMLIERGIADPDRLGIGGWSYGGLTTAWTLT
                     QTDRFKAAVLGAGVTNRVSMQGTTDIVTWHGSWQTAEFAEATDEYWHRSAMRYIGQVK
                     TPTLIPHGEKDDRVPVSQGWEYYNALRMMGVPTRMVVYPREPHGLTERQHQRDLMERV
                     LAWFDEYVKA"
BASE COUNT          315 a          710 c          636 g          361 t
ORIGIN      
        1 accaacgcgt cccgcgtccc acgtcccgac tctcacaccc gcctgaactt ctgcacccgc
       61 tggccctcca gcacctcgga gacgtagagg gagccttcgg agtcctgcca gagcgagtgg
      121 ggcgcgacga actggcccgg ctcgcgcagt tcctggccgc cgatgcgggt aatggcgtgg
      181 tcgtcctcgt cgaggatcgt cacccgtgag ctgagctccg agacgtagcg cacgccatcg
      241 gcatcgacga cgatgtcggt cgggcggcgg acgccaggga ccgtcccgcg atactcgccg
      301 tcgagcgaga agcgctggat gcgtgagttc tcgcggtcgc agacgtagac cgtctcctgc
      361 ccgttccagt tcgccatcca gacgccgtgc ggcagccgga actcgttcgg cgcgacgccg
      421 gggccgcccc aggtcctgat gtgctcgccg gtcgatgaga gaggatggac gagcgcgttg
      481 ccgtagccgt cggtgacgta gatgtcgcca ttcgacgcga cggctgcgtc ggtcgggcga
      541 ttgtagcggt cttcctgccg ttcccagacg acctcgccgt cgagcgtcgt ccgggcaatg
      601 gcgttgcggt cgcggtcaac gatcagcaga tactcgcctt cgccgtcctt gccgacgtag
      661 gccgagtgga tgtccgtgaa gcgaccgccc tccggccagg cgccgaggta ctcgccactc
      721 ttgtcgaaga tgctcactcc cggcgcgatc cgctggaaga agaagactcg gtcctgcgaa
      781 tcgacgacga cctggacaac ctgcccccag cgcattccct ccggcagctt cggccagccc
      841 tcaacaacct cgtacctgcg aattccggcg ccaacttcca tgggttgctc ccttcggtcg
      901 tcgggattcg ggatttggga ttcgggagca ttcaacggaa catgtccctt cgatgccccc
      961 gaatcccgaa tcccaaatcc cgcgcgtcac gccttcacat actcgtcaaa ccaggccagc
     1021 acgcgctcca tcagatcgcg ctggtgctgg cgttcggtca ggccgtgcgg ttcgcggggg
     1081 tagacgacca tcctggttgg tacgcccatc atccgcagcg cgttgtaata ctcccagccc
     1141 tggctgaccg ggacgcgatc gtccttctcg ccgtgcggga tcagcgttgg tgtcttgacc
     1201 tgaccgatgt agcgcatggc cgagcggtgc cagtactcgt cggtcgcctc ggcgaactcg
     1261 gccgtctgcc agctgccatg ccaggtgacg atgtcggttg tgccctgcat gctgacgcgg
     1321 ttggtgacgc cggcgccgag gacggccgcc ttgaagcggt cggtctgtgt cagcgtccag
     1381 gccgtcgtca gcccgccgta gctccagccg ccgatgccga gccggtccgg gtcagcgatg
     1441 ccgcgctcga tcagcatgtc cagcccacgc tgcgagtcaa ggtagtcgcc gcccatccag
     1501 tcggcgtagt tggcgatctg gaactcccag ccacggccgg tgctgccgcg cggattcggc
     1561 atcaggaccg cgtagccctg cccagccagg aactggcccc agtcgtgcca gctgcagtaa
     1621 aagtgatcgt cccaggccca gctcggtccg ccgtggatgt ggaggatcgt cgggtagcgc
     1681 gtgccttcct gatagccgac cgggtagatc agcaacccct cgatctccag ttcatcgctc
     1741 tgccagcgca ccagctcggt ccgcccgatc ttgcgcgacg tcagttcctc gttgaagtgc
     1801 gtcaactgac gcgcctcgcc acccaccacg ccggtccaga gctcgaccgg atgggtcgcg
     1861 tcgctccagt tgaacgcaaa ccgctcaccg gagcgatcga ggacgttggc aatgccggcc
     1921 gcttcccagc cgtagccggc cgttccgcgc gctccaagat ccccaccgac gagcttctcc
     1981 gggccgccgt cgccgtcggg cgcgagcatc cagatcgtcg tg
//
LOCUS       DASUGJ010000677         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1916215, whole genome shotgun sequence.
ACCESSION   DASUGJ010000677 DASUGJ010000000
VERSION     DASUGJ010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916215"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFV93_15380"
     CDS             complement(<1..245)
                     /locus_tag="VFV93_15380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166786.1"
                     /translation="MTGPATEEIVATVDAPVGRSNTTLWLLIGAIFVVSIDSRVITPI
                     LPAIADDLDVSVGRAGLIVTAYLLPYGLFQLIYGPVA"
     gene            complement(242..754)
                     /gene="sixA"
                     /locus_tag="VFV93_15385"
     CDS             complement(242..754)
                     /gene="sixA"
                     /locus_tag="VFV93_15385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX5166787.1"
                     /translation="MEIYIVRHGDAIDRNDPQITSDAMRWLTDEGRDEVAVSARLLQK
                     LGVRPDLVMTSPMVRARQTAEIISDRIGPAGGPVTSDDLVYGGSLAGVLEDILQHGKP
                     KSVVLAGHMPSVGALVGYLAWARPEAGVPFRTAEVARVDLPDDALEAGFGDLRWLIPP
                     KIARKLLGGG"
     gene            complement(785..2038)
                     /locus_tag="VFV93_15390"
     CDS             complement(785..2038)
                     /locus_tag="VFV93_15390"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX5166788.1"
                     /translation="MYRVVIVATIFATVFAIIGATVVSAAPVATNYFYQTWARTDKPV
                     ADRAADRTWMWGPEAYSDAFLEPYAESPEGERLVQYYDKSRMEITHPGDDGSSWYVTN
                     GLLVNELITGRMQVGDNQFEQRAPADINVIGDPDQDHPITYAMLGDFLDDPPREEGDR
                     LWERLAFTEDGELTWYVDHEYGSQGMRAGHFVPETNHTVAEVFWEFMNSTGPRYYYSI
                     LFGSGGYDDGQLFENPFYATGFPITEAYWAFAEVGGVGRDVLLQCFERRCLTYTPENP
                     VGWQVEAGNVGQHYFRWRYGDDASPTVAKDDIEITELWPGETFSKPGPVEEHITLRNG
                     EEYSVDLTNWVLTDQQDNHRYTFSDISIGPGEKLTIYSCGGAGSGEPGTLDLGFCDGW
                     WDWGDYATLYDPAGTLVAYRYQTSQ"
     gene            complement(2057..>3798)
                     /locus_tag="VFV93_15395"
     CDS             complement(2057..>3798)
                     /locus_tag="VFV93_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5166789.1"
                     /translation="WDDGAGWEVWVAGAAGQGVRQLTTDALLSAIDWTGDGAEIVFAR
                     RVEGGSGIAAVNLAGEVRQITPGPRDRSPQISPDGMTVAFLSGRAGAFDVWKVPLAGG
                     AATQLTEQTNPLDEPRWTPRWSPDGSWLSYVSSRSGERNNDDIWIVSSDGRENRQLTT
                     GLIVDTDPIWSPDGQRLAIVANSEVEHWYGDDLDLWLVDLDDVRPRRVTTGGGMTRKL
                     DGGAVSWSADGRFVYCLTHLNGNTNLAAVRVEDGLLTRVTNHDGVVSDFTIAPAGDAF
                     AIVIAGQTAPPDLAIITIDGGMPVPLTSSASRVTAPLTAPIRMPFQSYDGIYCDAYLY
                     LPVGFSAGRRFPALVQVHGGGTNAFGNGWHPIEQFLAQRGFIVFAVEYRGSSGYGRQF
                     AGLSYADWGGGQTLDAVAAAGFLRSKSYVSGVGIYGGSYGGYLSLHAVVAAPDAFGAA
                     ADMYGITNRFDYFDRSDRVGRVFVSRDYGGRGPVDAPELYLRASTHHRLGEIRTPLLV
                     LHGSEDTRVPPVQSEAVVEQLRAHGIEHEYVVYPGEGHGFRKREHRIDAYERLASWFE
                     RHLLRGSHRDSGD"
BASE COUNT          641 a         1262 c         1241 g          654 t
ORIGIN      
        1 tcggcgaccg ggccgtagat cagctgaaac aggccgtagg gcagcaggta ggcggtcacg
       61 atcagaccgg cccggccgac gctgacgtcc aggtcgtcgg cgatggccgg caggatcggc
      121 gtgatgacgc gcgagtcgat cgagacgacg aagatcgccc cgatcaacag ccagagcgtc
      181 gtgttcgacc ggccaaccgg cgcgtcgact gtcgcgacga tctcctcggt cgccggcccc
      241 gtcacccgcc gcccagcagc ttccgggcga tctttggcgg gatcagccag cgcaaatcgc
      301 cgaatcccgc ttccagcgcg tcgtcgggta gatcgacccg ggcgacctcg gccgtccgga
      361 agggcacacc tgcctccggc ctcgcccagg ccaggtagcc gaccagcgcg ccgacgctcg
      421 gcatgtgccc ggccaggacg accgacttcg gcttgccgtg ctggaggata tcttccagca
      481 ccccggccag cgagccaccg tagacgaggt cgtcgctggt gactggcccg ccggccggac
      541 cgatccggtc gctgatgatc tcagccgtct gccgggcgcg aaccatcggg ctggtcatga
      601 ccagatccgg acggactccc agcttctgca gcagccgcgc gctgacggcc acctcgtcgc
      661 gcccctcgtc ggtcagccag cgcatggcgt cggaggtgat ctgtggatcg ttgcggtcga
      721 ttgcgtcgcc gtggcgaacg atgtagatct ccattgttcc ccgtcgcctc tcactctgcc
      781 ggcgtcattg cgatgtctga tagcgatagg caaccagcgt cccggcagga tcgtagagcg
      841 tcgcgtagtc gccccaatcc caccagccgt cacaaaagcc caggtcgagc gttcccggtt
      901 cacccgatcc agcgccaccg caactgtaga tggtcagctt ctcgccgggg ccgatactga
      961 tgtcgctgaa cgtgtagcgg tggttgtcct gctggtctgt aaggacccag ttggtgagat
     1021 cgacgctgta ctcctcgccg ttgcggagcg tgatgtgctc ctcaaccggt cccggcttgc
     1081 tgaacgtctc acccggccag agttcggtga tttcgatatc gtccttcgcg accgttggcg
     1141 acgcatcgtc gccgtagcgc caacggaagt agtgctggcc gacgttaccg gcctcgactt
     1201 gccagccaac cgggttttct ggcgtgtagg tcagacagcg acgctcgaag cattgcagca
     1261 gaacgtcacg tccaaccccg ccgacctcgg cgaaggccca atacgcctcg gtgatcggaa
     1321 agcccgtcgc gtagaacggg ttctcgaaca attgtccatc gtcgtaacca ccactaccga
     1381 ataggatgct gtagtagtac ctcggaccgg tagaattcat gaactcccag aacacctctg
     1441 cgacggtgtg gttcgtttcc gggacaaaat gccccgccct catcccctgt gaaccgtatt
     1501 catgatcgac ataccaggtc agctcgccgt cttcggtgaa tgcgaggcgc tcccagaggc
     1561 gatcgccctc ttcccgtggc gggtcgtcga gaaaatcacc gagcatcgca tacgtgatcg
     1621 ggtggtcctg gtcggggtca ccgatgacgt tgatatcggc gggtgcgcgt tgctcgaatt
     1681 gattgtcacc aacctgcatc cggcccgtga tcagctcgtt gacgagcaga ccgttcgtga
     1741 cgtaccagga actgccgtca tcgccgggat gcgtgatctc catccgggac ttgtcgtaat
     1801 actggaccag ccgctcccct tcgggcgact ccgcgtacgg ttcgagaaac gcgtcgctat
     1861 acgcttcggg tccccacatc caggttcggt cggcggctcg gtctgcgacc ggcttgtcgg
     1921 tccgggccca ggtctggtag aagtagttgg tggcgactgg cgcggcggaa acgacggtgg
     1981 cgccgataat cgcaaagact gtcgcgaaga tcgttgccac gataacgacc cgatacatct
     2041 gccggactac ccttcttcaa tcaccgctgt cgcggtggct accacgtaat agatgccgct
     2101 cgaaccagga cgccaggcgc tcataggcgt cgatccgatg ttcgcgcttg cgaaagccgt
     2161 gcccctcgcc ggggtagacg acgtactcgt gctcgatgcc gtgtgcccgc agctgctcga
     2221 caaccgcttc ggactggacc ggtggaacgc gagtgtcctc gctgccgtgc aggacgagca
     2281 gcggagttcg gatctcgccg agccggtggt gggtcgaggc gcgcaggtaa agctccggtg
     2341 cgtcaaccgg gccgcgaccg ccgtagtcgc gcgagacgaa gacacgcccg acccggtcgc
     2401 tgcggtcgaa gtagtcgaag cggttggtga tgccgtacat gtcggcggcc gcgccgaagg
     2461 cgtccggcgc agcgacgacc gcgtgcagcg acaggtagcc gccgtacgag ccaccgtaga
     2521 tgccgacgcc tgagacgtac gatttcgagc gcaggaaccc ggcagcggcg acggcatcca
     2581 gcgtctgacc gccgccccag tcggcgtagg agaggccggc gaattgccgt ccgtagccgc
     2641 tcgatccacg gtattcgacg gcgaagacga tgaatccgcg ctgggccagg aactgctcga
     2701 tcggatgcca gccgttgccg aacgcgttcg tcccgccgcc gtggacctgg acgagcgccg
     2761 gaaaccggcg gccggcgctg aacccgaccg gcaggtagag ataggcgtca caatagatgc
     2821 cgtcgtagct ctggaacggc attcggattg gtgccgtcag cggggccgtc acccggcttg
     2881 cgctgctggt cagcggcacc ggcatgccgc cgtcgatcgt gatgatcgcc aggtcaggtg
     2941 gcgcggtctg gccggcgatg acgatcgcga aggcatcacc tgccggcgcg atcgtgaagt
     3001 cgctgacgac gccgtcgtga ttcgtgacgc gtgtcagcag gccgtcctcg acgcggacgg
     3061 cggccaggtt cgtattgccg ttcaggtgcg tcaggcaata gacgaagcgg ccatccgccg
     3121 accaactgac ggcgccgcca tccagcttac gtgtcatgcc gccaccggtc gttacccggc
     3181 gcgggcgaac gtcgtccagg tcgaccagcc agaggtcgag gtcgtcgccg taccagtgct
     3241 cgacctcgct gttggcgacg atcgccagcc gttgtccatc cggcgaccag atcgggtcgg
     3301 tgtcgacgat caggccggtc gtcaactgcc ggttctcgcg tccgtcggac gagacgatcc
     3361 agatgtcgtc gttgttgcgc tcgccggagc gggacgagac gtacgagagc cagcttccgt
     3421 ccggtgacca gcgcggtgtc cagcgcggct cgtcgagcgg gttggtttgc tccgtcagct
     3481 gcgtcgcagc cccgccggcc agaggaacct tccagacatc gaacgcgcca gccctgccgg
     3541 agaggaacgc gactgtcatg ccgtccggcg agatctgcgg cgagcggtcg cgcgggccgg
     3601 gcgtgatctg gcggacctcg ccggccagat tgacggcggc gatgccgctg ccgccctcga
     3661 cgcggcgggc gaagacgatc tcggccccgt cgccggtcca gtcgatcgcc gacaggagcg
     3721 cgtcggtcgt tagctgtcgc acgccctgac cggcagcccc ggcgacccag acctcccagc
     3781 cggccccgtc gtcccaga
//
LOCUS       DASUGJ010000678         5531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1083402, whole genome shotgun sequence.
ACCESSION   DASUGJ010000678 DASUGJ010000000
VERSION     DASUGJ010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083402"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1135
                     /locus_tag="VFV93_15400"
     CDS             212..1135
                     /locus_tag="VFV93_15400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5166790.1"
                     /translation="MNPSQETPRDLAIVVVHWNVADLLVGCLRSIAAEVQRSGLSVSV
                     TVVDCASPDERFREVVGAFDDVALLELAENRGYAAGCNAGIRSTGGEAVLLLNPDTEM
                     LPGSLLTLWETLHVAPHVAMVAPLLLNPDRSVQSAGYRFPGVANVFFDLFPVHPRLVE
                     SPLNGRVPVGDGVQPIRIDYPLGAAMLVRRAALDAVGLLDESYGMYSEEIDLARRLAE
                     DDWTTLLAPAAQVVHYGGQSTGQRPDAMHEALWNSRAIYYERYASPRQRAAIAALIRF
                     GLGRGDRQAPAGQREANARIRERFRQIARRR"
     gene            1132..1896
                     /locus_tag="VFV93_15405"
     CDS             1132..1896
                     /locus_tag="VFV93_15405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5166791.1"
                     /translation="MTTGGVVAIVLTLNEAEHLPDCLASLRLLTDRVIVLDSGSTDGT
                     QAVALAAGAEWHTRPFDGYASQRNAALELACDAEWVLFLDADERLTEAGASEIHAALA
                     SAGTEIAAFWLPRRNIFFGRELRGGGWWPDAQARLLRPGRAHYDPTRQVHEVLVVDGG
                     SRRLRVPMIHLNYASRREFVAKQRDYTRQRVDGSDDDDVPRLRALAGAPVREFWRRFV
                     RLKGYRDGLTGLFLAGVLAIEEARAVVLLRARVRQS"
     gene            1893..2846
                     /locus_tag="VFV93_15410"
     CDS             1893..2846
                     /locus_tag="VFV93_15410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5166792.1"
                     /translation="MSGRVAAVVVSYNVRDLLLECVASLDEARQRGELQTIVVVDNAS
                     HDGSAEAVRGRFPDLEVIEASNRGYGAGANLGIAATSEEYVLILNPDTVIPPGTVAVL
                     SALLGNRPDVALAGPRLRYPDGSIQPTIRRFPERLTPLFESTIFAAWWPDNGWSRRFH
                     MRDVAGGLDGAREVDWVVGAAMLVRRAAIVEAGGFDESFLMYSEEVEWCWRLRRHGWA
                     IVYEPSVEIIHHEGASTSQDVPSRQVAFDTSRIRLARRMYGPGTAAVVRAGLLAGYGL
                     QLVREAIKWLLGHRRDLRRQRILLYLGALRTGLRERDGRAP"
     gene            2843..3925
                     /locus_tag="VFV93_15415"
     CDS             2843..3925
                     /locus_tag="VFV93_15415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5166793.1"
                     /translation="MSRPKVLFVSGEYPPMPGGVGDYTANLRMALAAMGVRSAVLASG
                     RATGEDVWTVRRWAWSSIASVLRRARREQVDIVHIQYQTGAFDMHPALNVLPSIVARR
                     LPVVTTFHDLRVPYLFPKAGRLRKSAIVRTARGSDAVIVTNPADGRALGNEGVAVTQI
                     PIGPNLPPPGPVAVGDEQTVAFFGFPARSKGILDLVEALAQLPEPRPGLTLIGAQGEP
                     GAGNDVVPAGEFDTLAARAGIAIQRTGYLTPAAASAALAQATVIALPFADGASLRSGS
                     LLAALQSGRPVVTTAPRTASDLAELATMPQLLLVPRGDRIGLATAIARGLNWTGQPAS
                     LPASFRWQTIAEAHVALYRQLLDARR"
     gene            3922..5463
                     /locus_tag="VFV93_15420"
     CDS             3922..5463
                     /locus_tag="VFV93_15420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5166794.1"
                     /translation="MMTTRTVRPAARTAAIGDLTVIAHYLALAAIVIAGAALRLTALN
                     RQSLWFDEIDVVVRAQQPLGTVMRTFVAEGENGPLYNILLAIWIRLAGISEIAVRFPS
                     AVAGTLAIPLIYLLARRLAGSTAGLLAAGLLAISPYHVWYSQEAKMYALVVLLALASS
                     VCLVEALERNRALWWAAYAIVTTLMFYTHVATVLVFVAQSAYVLLTRRGWRGRERGWL
                     VAAGVLTLPYLPIALWALRVVGGGVQTWQPDVGLWDAARILGIKFAVNRTDAVFETRG
                     ALLYAALAVGGAVVLAMRRERQRWWLLLVGLSAGPVVGLYLVSLRQSVFSDRYAIVAL
                     PAYLILVAAAVAWLVRRRMLWPLGLLAAFLLLAFAWGPLRDVNRSSAAEKEDWRSAYA
                     WVANRAQPGDVLLLHPGYIITTYDYYSQREPRLQQYPIATIPSFKVMWLDEPLMVQMI
                     REQVGEARRYWLIESPVRAEGEDPNARLAGWLQRRGTLLDQHEVNGVRIRLYELDEPP
                     APERP"
BASE COUNT          782 a         1807 c         1960 g          982 t
ORIGIN      
        1 gcttgatgta tccaacgttt cgcacgacgc gcatggcgga tcaactgctc ctctcggcag
       61 aggtgaatgg cgatgtcatc gacatgctga ttcagggttc cgacgacgtt cgtccgaact
      121 gcatgcagtc tagcatggca gggacggcac accaacgtac gcggcggcca acgcggcgca
      181 atcgtcgatc caccccgcgt cggggccgat cgtgaatcct tctcaggaaa cgccgcgtga
      241 tctggcgatc gttgtcgtcc actggaatgt cgccgatctg ttggttggct gcctgcgctc
      301 tatcgcggcg gaagtacaac gttcggggct gtccgtcagc gtcacggtcg tcgattgcgc
      361 gtctccggat gagcgctttc gggaggtcgt gggagcgttc gacgatgttg cgttgctgga
      421 gctggccgag aaccggggct atgcggcggg gtgcaacgcg gggatccgga gcaccggtgg
      481 cgaggcggtg ctgctgctga atccggatac ggagatgctg ccgggctcgt tgttgacgct
      541 ctgggagacg ctgcacgtcg cgccgcatgt cgcgatggtt gcgccgctgc tgctgaaccc
      601 ggatcgatcg gtgcagtcgg cggggtaccg gtttcccggt gttgccaacg tcttcttcga
      661 tctgttcccg gttcatccgc ggctggtcga atcgccgttg aacggccggg tgccggtcgg
      721 tgatggggtg cagccaatcc ggatcgacta tccactgggg gcggcgatgc tggtgcggcg
      781 ggcggcgctc gacgcggttg ggttgctgga cgagtcgtac ggcatgtaca gcgaggagat
      841 cgatctggcg cggcggctcg ccgaggatga ctggacgacg ttgctggcgc ccgccgcaca
      901 ggttgttcac tacggtgggc aatcgaccgg ccagcggccg gacgccatgc acgaggcgct
      961 ctggaacagc cgcgcgatct actatgagcg ctacgcatcg ccgcgccaac gggccgccat
     1021 cgccgcgctg attcgcttcg gcctcgggcg gggtgaccgg caggcaccgg ccgggcagcg
     1081 cgaggcgaac gcgcgcatcc gcgagcgctt ccggcagatc gcgagacggc gatgacgact
     1141 ggcggagtag ttgcaatcgt cctgacgctc aacgaggcgg agcatctacc ggattgcctg
     1201 gcatcgctgc ggttgctgac cgaccgggtc atcgtcctcg attctggttc gacggatggc
     1261 acgcaggccg tcgcgcttgc agctggcgcc gagtggcaca cccgcccgtt cgacggctac
     1321 gccagtcagc gcaacgccgc acttgagctg gcttgtgatg cggagtgggt gctgtttctc
     1381 gacgccgacg aacggttgac cgaagcggga gcgtctgaga ttcatgccgc gctcgcatcg
     1441 gccgggacgg agattgccgc gttctggctg ccacgccgca acatcttctt cgggcgcgag
     1501 ctacgtggcg gcggctggtg gccggacgcc caggcgcggc tgctgcggcc aggacgggcg
     1561 cactacgacc ccacgcggca ggtccacgaa gtgctcgtcg tcgacggcgg gagccgccgg
     1621 ttgcgcgtgc cgatgatcca cctcaactac gccagccgcc gcgagtttgt cgccaagcag
     1681 cgtgactaca cccggcagcg ggtcgacggt tccgatgatg acgacgtacc ccggctgcgt
     1741 gcgctggccg gcgctcccgt gcgcgagttc tggcggcgat tcgtgcggct gaagggctat
     1801 cgtgatgggc tgaccggcct gttcctggcc ggtgtgctgg cgatcgaaga ggcgcgcgcg
     1861 gttgtgttgc tgcgtgcgag agtgcggcag tcatgagcgg acgtgttgcc gccgtcgttg
     1921 tctcatacaa cgtgcgcgac ttgctgctgg aatgtgtcgc gtcgctggac gaggcgcggc
     1981 agcggggcga gttgcagacg atcgtcgtcg tcgacaacgc cagccacgac gggagcgccg
     2041 aggccgtgcg cggtcgcttt cccgatctgg aagtcatcga ggcgtcgaac cggggctacg
     2101 gcgcgggcgc gaatctcggc atcgccgcga caagcgagga gtacgtgctg atcctgaacc
     2161 cggacaccgt catcccaccc ggcacggtcg ctgtgctgtc ggcgctgctg ggcaaccggc
     2221 ccgacgtcgc actggccggc ccgcgcctgc gctacccgga cggcagcatc cagccgacga
     2281 tccggcgctt cccggagcgg ctgactccgc tctttgagag tacgatcttc gcagcgtggt
     2341 ggcctgacaa cggctggtcg cgtcgctttc acatgcgcga tgtggccggt ggactggacg
     2401 gagcgcggga ggtcgactgg gtggttgggg cggcgatgct ggtgcgacgg gcggcgatcg
     2461 tggaggccgg cgggttcgac gagtcgttcc tgatgtactc ggaagaggtc gagtggtgct
     2521 ggcgcctgcg ccggcacggc tgggcgatcg tctacgagcc atcggtcgag atcatccacc
     2581 acgaaggcgc cagcaccagt caggacgtgc cgtcacgaca ggtcgcgttc gacacgagcc
     2641 gaatccggct ggcacggcgg atgtacggtc ccgggacggc ggcggtcgtt cgggccgggt
     2701 tgctggccgg ctacggcctg cagctcgttc gtgaggcgat caagtggctg ctgggccatc
     2761 gccgtgatct gcgccggcaa cggattctgc tgtatctcgg tgcgctgcga accgggttgc
     2821 gcgagcgcga cgggcgggcg ccatgagcag gccgaaggtg ttgttcgtca gcggtgagta
     2881 cccacctatg ccgggcggcg tcggtgacta caccgccaac ctgcggatgg cgctggcggc
     2941 gatgggcgtg cgctcggcgg tcctcgcctc aggtcgcgcg accggcgaag atgtctggac
     3001 tgtccgacgc tgggcttggt cgagtatcgc cagcgtcctg cgccgtgcgc ggcgggagca
     3061 ggtggatatc gtccacatcc agtatcagac cggcgcattt gacatgcacc cggcgctgaa
     3121 cgtcctgccc tcgatcgtcg cgcgacggct gccggtcgtg acaacgttcc acgatctgcg
     3181 ggtgccgtat ttgttcccga aagcaggtcg gctgcgcaag tcggccatcg ttcggacggc
     3241 gcggggttcc gatgcggtga tcgtcaccaa tccggccgac ggccgcgcgc tcggcaacga
     3301 aggcgtcgcc gtcacgcaga tcccgatcgg gccgaacctg ccgccgcccg gaccagtcgc
     3361 ggttggcgat gaacagacgg tcgccttctt cggcttcccg gcgcgatcga agggcattct
     3421 cgacctggtc gaggcgctcg cgcagttgcc cgaaccgcgt ccgggactaa cgctgatcgg
     3481 cgcacagggc gaacccggcg cggggaacga cgtcgttccg gcaggcgagt ttgacacgct
     3541 ggcggctcgg gccgggatcg cgattcagcg aaccggctat ctcacgccgg cagccgccag
     3601 cgccgccctc gcccaggcaa ccgtcatcgc cctgccgttc gcggacggcg ccagcctgcg
     3661 cagtggctca ctactggccg cgctccagtc cggtcgcccg gtcgtgacga cagccccacg
     3721 gacggcctcg gatctcgccg aactggcgac gatgccgcaa ctgctgctcg tcccgcgtgg
     3781 ggatcggatc ggcctggcaa ctgccatcgc tcgcgggctg aactggacag ggcagcccgc
     3841 gtcgctgccg gcctccttcc gctggcaaac gatcgcagag gcgcatgttg cgctctaccg
     3901 tcaactgctg gacgcccggc gatgatgacg actcgaacgg ttcggccggc cgcgcggacg
     3961 gctgccatcg gcgatctgac ggtgatcgcc cactatctcg cgctggccgc tatcgtcatc
     4021 gccggggcgg cgttgcggct gacggcgctc aaccgccaga gcctctggtt cgacgagatc
     4081 gatgtcgtcg ttcgcgccca gcagccgctg gggactgtca tgcgcacctt cgtcgccgag
     4141 ggcgagaatg gcccgctcta caacatcctg ctcgccatct ggatcaggct ggccgggatc
     4201 tcggagatcg cggtccgttt cccgtcagcc gtggccggga cgctggcgat tccgctcatc
     4261 tacctgctgg cgcggcggct ggccggctcg acggccgggt tgctggcggc cgggttgctg
     4321 gcgatctccc cgtaccacgt ctggtactcg caggaagcca agatgtacgc gctggtcgtc
     4381 ctgctggcgc tggcatccag cgtctgcctg gtcgaggcgc tggagcgcaa ccgcgcgctc
     4441 tggtgggccg cctacgcgat cgtgacgacg ttgatgttct acacccacgt cgcgaccgtc
     4501 ctcgtcttcg tcgcgcagtc ggcctacgtg ctgctgacgc gtcgtggctg gcgtggccgg
     4561 gagcggggct ggctggtcgc cgctggcgtg ctgacgctgc cctacctgcc gatcgcgctc
     4621 tgggcgttgc gggtggttgg cggcggtgtc cagacctggc agccggacgt cggtctctgg
     4681 gatgcggccc ggattctcgg gatcaagttc gccgtcaatc ggaccgacgc cgtcttcgag
     4741 acacgtgggg cactgctcta cgccgcgctg gcggtcggtg gggcggtcgt gctggcgatg
     4801 cggcgggagc ggcaacgctg gtggttgctg ctggttggcc tcagcgccgg cccggtcgtc
     4861 ggcctgtatc tggtctcgct gcgtcaatca gtcttctccg accgctacgc gatcgtcgcc
     4921 ctgccggcct acctgatcct ggtcgcggca gccgtcgcct ggctcgttcg ccggcgcatg
     4981 ctctggccgc ttggcctgct ggcggcattc ctgctgctgg ccttcgcctg gggaccgctc
     5041 cgcgatgtca accgctcgtc ggccgccgag aaagaggact ggcgtagcgc ctacgcctgg
     5101 gtggcaaatc gcgcccagcc gggcgatgtc ctgctgctgc atcccggcta catcatcacg
     5161 acctacgact actacagcca gcgcgaaccc cgcctgcagc agtacccgat cgcgacgatc
     5221 cccagcttca aggtcatgtg gctcgatgag ccgctgatgg tccagatgat ccgcgagcag
     5281 gtcggcgaag cccgccgcta ctggctgatc gagtcgccgg tccgcgccga aggcgaagac
     5341 cccaacgccc gcctggccgg ctggcttcag cgtcgcggca cgctgctcga ccaacacgag
     5401 gtcaacggcg tccgcatccg cctctacgaa ctggacgaac ctccggcgcc ggagcggccg
     5461 tagccaatca atccaatccc caatccggta cgggcgatat cgcaatatcg cccttgtcga
     5521 cgcgccgggt g
//
LOCUS       DASUGJ010000679         4076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_109928, whole genome shotgun sequence.
ACCESSION   DASUGJ010000679 DASUGJ010000000
VERSION     DASUGJ010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109928"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFV93_15425"
     CDS             complement(<1..319)
                     /locus_tag="VFV93_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166795.1"
                     /translation="MNVRRLVTAGSLGLLALMLLVMPAAAGVSWCRADPIVELNGTSV
                     QVWVAVGEEHEHLVSGPIQVTVYTPDRVRTRTIFLDEGFNGFGEVVDFRNRGWLDRNG
                     RFDV"
     gene            complement(350..832)
                     /locus_tag="VFV93_15430"
     CDS             complement(350..832)
                     /locus_tag="VFV93_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166796.1"
                     /translation="MRHLIVSFCVALVAALTVVAPASAGRTWCSRDPVVTLAGHDVQV
                     LVAIPAEYVHLVDGPIDVVFKTPSGMTRSVAFTDDGFNGFGETVSFVDNPRSPVNAKG
                     NFTLDVRVTVPIDESQTTAELKTDKIPTWITVTEGNTSVVLHGWSDGSWVTARIGNGR
                     "
     regulatory      complement(893..977)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1177..1839
                     /locus_tag="VFV93_15435"
     CDS             1177..1839
                     /locus_tag="VFV93_15435"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEX5166797.1"
                     /translation="MASSIDMDLSRSDLPAIDHAGLSDEQLAELIVSRAYDDESRRER
                     KPLPRWLGPAMRALPKVLAIVTVANGLIGLLAVLAPWLRMAAGDAATQPLYSAYSLIC
                     PQRDSHTWFIGDVPMAMEQRMVAMYLAFGVAGAAYLFWERFRRPLPTWVMLLSVAPAL
                     IDVAISTAGIRHSTEISRLWTGTLASLGIVFWVYPRFDAQLRVVQAKVAELRAAVAQR
                     PG"
     gene            2024..>4076
                     /locus_tag="VFV93_15440"
     CDS             2024..>4076
                     /locus_tag="VFV93_15440"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX5166798.1"
                     /translation="MVERIAHDAIAGDVAPDGLTVTLLGGFSVAVGSRVIAPSSWRLR
                     KAGQLVKLLALAPQRRMHREQALDTLWPHLDPTAAGNNLRVTLHFARKLLDPEPEAGS
                     GALQVVGELLVLFPGREVVVDVDRFEELARTALRSDDPVAYTAALDLYGGELLPEDRF
                     EEWAIERRTALQSLQLTLQRNLARLYEARGETDHAIETLRAALAIDPVAEALHLALMR
                     LYTKAGRRAQALRQYQRLRETLQRELDIEPDPETTRLYEAILTGTATDFLQPIGGVLR
                     ETAAGHDSVVRRTAPQEPRTNLPAQLTSFVGREHELDRLSQALDTARLVTLTGPGGAG
                     KTRLAIEVAGPLLEAYAGGIWLVDLTSLSDAALVPRTVAVALGVQEAEGRQPIDTLAD
                     ALGGKRLLLVLDNCEHVIAAAAELADQLLRRCPQLRMLATSREALRVPGEVVWPVPAL
                     AVPDAEHLPPIEVLARVEAVQLFVERARLSAPDLRISTTNAVAIAAICERLDGLPLAI
                     ELAAARVNSLPVGQLAARIDQALPLLTGGSRPGPARHRSLQATISWSYDLLSPPEQRL
                     LARLAVFAGSFSIEAAEAICSDQQVPAGRVLDLLFQLVDKSLVVVDEHDGEVRYRLLE
                     TIRQFARELLERSDERDTAQRAFRDWYRALVERAEPELLGPGNVPWMRRIWSEIDNIR
                     VV"
BASE COUNT          662 a         1370 c         1347 g          697 t
ORIGIN      
        1 cgacgtcgaa gcgcccattg cggtccagcc agccccggtt gcggaaatcg accacctcgc
       61 caaagccgtt gaagccttcg tcgaggaaga tcgtgcgcgt ccggacccgg tccggcgtgt
      121 agaccgtgac ctggatcggg ccggagacga gatgctcgtg ctcttcgccc accgccaccc
      181 agacctggac cgaggtgccg ttcagctcca cgatcggatc ggcccggcac cagctcacac
      241 cggcagccgc cggcatgacc agcagcatca gcgccagcaa cccgaggcta ccggctgtca
      301 ccaatctgcg aacgttcatc gtctgaatgc ctcttcccat cctgaccgac tagcggccgt
      361 tgccgatgcg ggccgtaacc cacgatccat cgctccagcc gtggagcacg actgacgtgt
      421 ttccctctgt cacggtgatc caggttggga tcttgtcagt cttcaattca gccgtcgtct
      481 gcgactcatc aatcgggacc gtcacccgga catcgagcgt aaagttgccc ttcgcgttca
      541 ccgggctgcg tgggttgtca acaaaggaga cggtctcgcc aaatccgtta aacccgtcat
      601 cggtgaaggc gaccgaccgg gtcatgccgg acggcgtctt gaagacgaca tcaattggtc
      661 cgtcgacgag gtggacatat tcggcgggga tcgcaaccag cacctggaca tcatgtccag
      721 cgagggtcac gaccgggtcg cgagagcacc aggtgcggcc cgccgacgcc ggagcgacga
      781 cagtcagggc ggccaccaga gcaacacaga aactgacaat gaggtgtctc atcagcccac
      841 aatccctcac ccgccacaac gacgggtgac atgaaacagg cgatgagaag tcttcggtag
      901 cgcagccgtc atggcgaaat cgccgaagac ctgtcgcttt gcggcctcac gtttccgcga
      961 gtgtgccttt gtcgtgacgt cccatccgaa catcacagca tggatccgat cttagacagg
     1021 acttgctccg gtcaacagag tcattggtcc ggatcgggga cgtactgagg caccgatccg
     1081 gcaaacagga ccgaggtcat ctgtcgcgtc ttccaaacaa ttcctagact tgcaggtact
     1141 gacgttggct cgtagcagag actggtgctg tcacaggtgg cttcttcgat agatatggac
     1201 ctgagccgtt ccgatctccc ggcgatcgat cacgccgggc tctccgacga gcagctggcc
     1261 gaactgatcg tgtcacgcgc ctacgacgac gaatcccggc gcgagcgcaa gccgctcccg
     1321 cgctggctcg gcccggcgat gcgtgccctg ccgaaagtgc tggcgatcgt gacggttgcc
     1381 aacgggctga tcgggctgct cgccgtcctc gccccctggc tccggatggc cgccggcgat
     1441 gctgccacac agccgctcta cagcgcctac tcgctgatct gtccgcagcg cgactcgcac
     1501 acctggttca tcggcgacgt gccgatggcg atggagcagc ggatggtggc gatgtacctc
     1561 gccttcggcg tggccggagc cgcctacctg ttctgggagc ggtttcgccg cccgctgccg
     1621 acctgggtga tgctcctgtc ggtcgcgccg gccctgatcg acgtcgccat ctcgacggcc
     1681 ggcatccgcc acagcaccga gatctcccgc ctctggactg gcacgctcgc ctcgctcggc
     1741 atcgtcttct gggtctaccc acgcttcgac gcgcaactgc gcgtggtcca ggccaaagtc
     1801 gccgagttgc gcgccgcagt tgcccaacgc cctggatagg gccgcgtcct gcgccggcct
     1861 ctaccggatt cgtgatgcag caccggggtt gtcggcgctg cctcagcgcc gctggattga
     1921 cagccgggcc ggcacgcccg tatcctgccg ccacattccc attatcgtga tgagccgccg
     1981 ttgccggtct gacgcagttg cctgctggaa cattcggtga acgttggtcg agcggattgc
     2041 ccacgacgcg attgccggcg atgtcgctcc tgatgggttg accgtcacgc tgctcggcgg
     2101 gttcagcgtc gccgtcggtt cgcgcgtaat cgccccgtcg agttggcggt tgcgcaaggc
     2161 tggtcagctg gtcaagctgc tggcgctggc gccgcaacgc cgaatgcacc gcgaacaggc
     2221 gctcgacacg ctatggccgc acctcgatcc gaccgccgcc ggcaacaacc tgcgggtgac
     2281 gcttcacttc gcccgcaagc tgctggaccc ggagccggaa gcgggctccg gagccttgca
     2341 ggtcgtcggc gagttgctgg tgctctttcc cggccgcgag gtcgttgtcg atgtcgaccg
     2401 cttcgaagaa ctggcacgga ccgcgctacg ttcggacgac ccggtcgcct acacggcagc
     2461 gctcgatctg tacggcggcg agttgctgcc ggaggaccgc ttcgaggagt gggccatcga
     2521 gcggcggacg gcgttacagt ctctccagct gacgttgcag cgcaatctgg cgcgcctcta
     2581 cgaggcacgc ggcgagacgg accacgcgat cgagacgctg cgggcggcgc tggccattga
     2641 tcccgttgct gaagcgctcc atctcgcgct gatgcggctc tacaccaagg ctggaagacg
     2701 tgcccaggcg ctgcgccagt accagcggct gcgcgagacg ttgcagcgcg agctggacat
     2761 cgagccagat ccggagacga cgcgcctcta cgaggcgatc ctgacgggca ccgccaccga
     2821 cttcctgcaa ccgattggtg gtgtcctgcg tgagaccgca gccgggcatg attcggttgt
     2881 gcgacgaacg gcgccgcagg agccacgcac caacctgccg gcgcaactga ccagcttcgt
     2941 cgggcgcgag catgagctgg acaggctcag tcaggcgttg gatacggcga ggctcgtcac
     3001 gctgaccggc cccggcgggg cgggcaagac gcgactcgcg atcgaggttg ccggcccgct
     3061 gctggaggcg tacgccggcg gcatctggct cgtcgatctg accagcctca gcgacgccgc
     3121 gctcgtgccg cggacagttg ccgtcgcgct gggtgtccag gaagcggagg ggcggcaacc
     3181 gatcgacacg ctggccgatg cgttgggcgg caagcggctg ctgctggtcc tcgacaactg
     3241 cgaacacgtc atcgcggcgg cggccgagtt ggccgaccag ctcctgcgac gttgcccgca
     3301 actgcggatg ctggcgacca gccgcgaggc gctccgcgtg cccggcgagg tcgtctggcc
     3361 ggtcccggcc ctggcggttc cggatgccga gcacctgccg ccgatcgagg tgctggcgcg
     3421 ggtcgaagct gtccagctat tcgtcgagcg cgcacggctc agcgccccgg atctgcggat
     3481 cagcaccacg aacgccgtcg cgatcgccgc catctgcgaa cggctggacg ggctgccgct
     3541 ggcgatcgag ctggcggctg cgcgggtgaa ctcactgccg gtcggccagc tggctgcccg
     3601 gatcgatcag gcgttgccgt tgctgacggg cggcagtcgg cccggtcccg cccgtcaccg
     3661 gagcctgcaa gcgacgatca gttggagcta cgacctgctc agcccgccgg agcagcggct
     3721 gctggcgcgg ctggccgtct tcgccggcag cttctcgatc gaggcggccg aggcgatctg
     3781 ttcggatcag caggtgccgg ccggccgggt ccttgacctg ctctttcagc tggtggacaa
     3841 gtcactggtc gtcgtcgatg agcacgacgg cgaggtgcgc tatcgactgc tggagacgat
     3901 tcgccagttt gcgcgggagc tgctggaacg cagcgacgag cgggacacgg cccagcgtgc
     3961 cttccgcgac tggtaccggg cgctggtcga gcgggccgaa ccggagctgc tcgggccggg
     4021 caatgtgccc tggatgcgac ggatctggtc ggagatcgac aacatccgcg tcgttc
//
LOCUS       DASUGJ010000680         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_666363, whole genome shotgun sequence.
ACCESSION   DASUGJ010000680 DASUGJ010000000
VERSION     DASUGJ010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666363"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..513)
                     /locus_tag="VFV93_15445"
     CDS             complement(4..513)
                     /locus_tag="VFV93_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX5166799.1"
                     /translation="MSQSVEEHRASAPESVGCVVITVSDTRTVETDRSGQIMRERLER
                     AGHRVVGYEIVKDEPDQINRVLDQYTSTDGCQAILFNGGTGIARRDTTFDVISRRLEK
                     TLPGFGELFRMLSFDEIGAAAMLSRATAGVLNGRLVFSTPGSSNAVALAMDKLIAGEL
                     AHLVFEGTK"
     gene            complement(563..931)
                     /locus_tag="VFV93_15450"
     CDS             complement(563..931)
                     /locus_tag="VFV93_15450"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX5166800.1"
                     /translation="MARTTDAGNAGEAFVAGRLVRAGYRIVDRNWRIRGGEIDIIALD
                     GETLVFVEVKVRTGERIGYAEDTLDARKLSRLMVAAEQYVNLHSAHQDRLWRVDFVAV
                     TLSPTGVVRRYSHVENVTLE"
     gene            complement(915..1625)
                     /locus_tag="VFV93_15455"
     CDS             complement(915..1625)
                     /locus_tag="VFV93_15455"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX5166801.1"
                     /translation="MDAVATANGRRTRTPIDRAPTLRWEQQAWRDGKLLVAGVDEAGR
                     GAWAGPLVAAAVILPRDPQRRAKITRAINRAETAVRDSKQLSADQRQRVMAVLLNLEI
                     PFAVAVIDVAELDAIGLGRANKLAMCNAIGALSPEPEHVLVDAFRLDALPCSHDPIVH
                     GDNLSQTIAMASIVAKLHRDAIMEQLDLEFPQYSFAAHKGYGTVAHRVALERHGISPQ
                     HRRSFAPIAELIARGADD"
     gene            complement(1702..>2070)
                     /locus_tag="VFV93_15460"
     CDS             complement(1702..>2070)
                     /locus_tag="VFV93_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX5166802.1"
                     /translation="VLLDLEIPFAVAVIDVAELDSIGLGRANKLAMCNAIGALSPEPE
                     HVLVDAFRLDTLPCSHDPIVHGDNLSQTIAMASIVAKLHRDAIMEQMDLEYPQYAFAA
                     HKGYGTAEHRTALEWLRTLD"
BASE COUNT          377 a          714 c          617 g          362 t
ORIGIN      
        1 ccgctacttc gtgccctcga agaccaggtg cgccaactca ccggcaatca gcttgtccat
       61 tgccagcgcg accgcgttcg acgaaccggg tgtggagaag acgagccggc cgttcaggac
      121 gccggcagtt gcccgcgaga gcatcgccgc cgcgccgatc tcgtcaaacg acagcattcg
      181 gaacagttca ccgaaacctg gcaaggtctt ctccaggcgg cgtgagatga catcaaacgt
      241 ggtgtcgcgc cgcgcgatac cggtcccacc gttgaagaga atcgcctgac agccgtcggt
      301 ggaggtgtac tggtccagca cacggttgat ctggtccggc tcgtccttga cgatctcgta
      361 tccaacgacg cggtggccgg cacgttctag ccgttcgcgc atgatctgac cgctgcggtc
      421 cgtctcgacc gtacgcgtgt cactgacggt gatgaccaca cagccgaccg actccggcgc
      481 gcttgcccga tgctcctcga cagactgact catccgccgc tcgtcccttc ctgaatcagg
      541 atcgctcaat cgaccacgcc gatcattcga gcgtcacatt ttcgacgtgg ctgtagcgtc
      601 gaacgacgcc ggtcggcgac aatgtcaccg cgacgaaatc gacccgccag agccgatcct
      661 gatgcgcaga atgcagattc acgtactgct cggcagccac catcagccgc gacagcttgc
      721 gcgcgtcgag cgtatcctcg gcgtaaccaa tccgctcgcc ggtccggacc ttgacctcga
      781 cgaacacgag cgtctcgccg tccagcgcga tgatgtcgat ctcgccgccc cgaatgcgcc
      841 aattgcgatc gacgatccga taccctgccc gtaccagccg gcctgccacg aacgcctcac
      901 cggcgttgcc ggcatcagtc gtccgcgcca cgagcgatca gttccgcgat cggagcgaac
      961 gacctgcggt gctgcgggga tatgccgtgc cgctccagcg cgacccggtg tgcaaccgtc
     1021 ccgtatccct tgtgggcggc aaacgagtac tgcgggaatt cgaggtccaa ctgctccatg
     1081 atcgcgtcgc ggtgcagttt cgcgacgatc gacgccatcg cgatcgtttg cgacagattg
     1141 tcgccgtgga cgatcggatc gtgactacac ggaagtgcat ccagccgaaa cgcgtcgacc
     1201 aggacgtgct ccggttctgg cgacagtgcg ccaatcgcgt tgcacatcgc cagcttgttg
     1261 gcgcgcccca gaccgatcgc atcgagttcc gcgacatcga tcaccgcaac ggcaaacgga
     1321 atctcaagat tcagcagcac agccatgacg cgctgccgct ggtcggccga gagctgcttc
     1381 gaatcgcgaa cggccgtctc ggcccggttg atggcgcgcg taatcttcgc ccggcgttgt
     1441 ggatcgcgag gaaggatgac cgcagccgcc accaatgggc cggcccatgc gccccgcccc
     1501 gcctcgtcga cgcccgccac cagcagcttc ccgtcacgcc aggcctgctg ctcccagcgc
     1561 agcgtcggag cccggtcgat gggtgttcgc gtccggcgtc cgttggcggt cgcaaccgcg
     1621 tccaatcgtg cagcgctcca atcgtcattc ggcgtcgctc gattctagcg cacgacgacc
     1681 cgggaagcgg ccggcgaccg gtcagtcgag cgtgcgcagc cattcgagcg cggtccggtg
     1741 ttcggccgtc ccgtatccct tgtgagcggc aaacgcgtac tgcgggtatt cgaggtccat
     1801 ctgctccatg atcgcgtcac gatgcagctt ggcgacgatc gacgccatcg cgatcgtctg
     1861 cgacaggttg tcgccgtgaa cgatcggatc atgactgcag ggcagggtat ccagccgaaa
     1921 cgcgtcgacc agcacatgct ccggctccgg cgacagtgcg ccaatcgcgt tgcacatcgc
     1981 cagcttgttg gcgcgcccca ggccgatcga atcgagttcc gcgacgtcga tcaccgcaac
     2041 ggcaaacgga atctcaagat ccagcaatac
//
LOCUS       DASUGJ010000681        11584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_251534, whole genome shotgun sequence.
ACCESSION   DASUGJ010000681 DASUGJ010000000
VERSION     DASUGJ010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11584
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251534"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="VFV93_15465"
     CDS             <1..1657
                     /locus_tag="VFV93_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX5166803.1"
                     /translation="LFLNPGTDTFPVQEALRKLEVLGRKTPRTVLCLFEIVALAAAHG
                     YYAATGRPQAVLVHVDVGTQNLGSMLHNAQRGRAAVVIVAGRAPYTTDAEQRGSRSSY
                     IHWLQEQRDQHGIVRNYTKWDYELRRADQVGEVVSRAFQVAASDPPGPVYMTLPRELL
                     METVGEVSIHQPGRFPPARLGAGDPDALREVARRLVASERPLVLTGNSGRTEAGYHGL
                     IRLAELLALPVVEWRNRANFPGDHPLHLGYAVRPLLDDADVVLALDHDYPYIPTTAQP
                     AESAFIVQLDLDPVKEQVPLWTFPVDLPIRGDTSRALDLIADEAERLLTGAQRERIEA
                     RRNALTIQHEELRETWRAEALAGEATLPITIPWLARCLRELQDDAPDLIVVDEMVTSN
                     ETVWKQFPVRTPGSWYGSGGSGLGWGLGASLGVKLAHPDRPVLALVGDGSFMFGTPTA
                     ALWAQQTQQAPVLTVILNNTCYNATKRPLVANYPAGYSVREDSFIGVDLTPAPRYDKL
                     ADVVGAYGERVESPADVLPALQRALERTRSGQSVVLDVQLAHP"
     gene            complement(1723..3141)
                     /locus_tag="VFV93_15470"
     CDS             complement(1723..3141)
                     /locus_tag="VFV93_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5166804.1"
                     /translation="MATIETTRPGLTAQVIESLSASHRGELLQPGDPAYDDAREVHNG
                     LIDRRPALIARCTGVADVISAVNVARDNNLVLSIRGGGHNVAGNAVNDGGIVIDLSRM
                     RGVRVDLKAGTVRAEGGATWGDVDHETQMFGLAVPGGVVSSTGIGGLTLHGGLGWVRR
                     KYGLSIDSLISVDIVTADGQLRTANSEENRDLFWAVRGAGSNFGVVTSFEFAMHPVGP
                     MVALAATAYAPEDGPRVLAGWRGFMDAAPEEISGSAVFWCLPPADFIPAEMHHRPVLL
                     VAALHTGAVDEGEAALVPLRHLAEPVLDMSGTVPYTELQSSFDPLFPKGWFYYWKSLY
                     LNKLEEPEIAALTQIGANRPTPITAVALWHLGGQMTRTAASETAFGSREAPYLLSLDT
                     TWNNEADTDRCIQWTRDTWRAAHAFSQGGLYLNFAGFGEEKEALVRASYGTNYDGLVD
                     IKTRYDPTNLFRMNNNISPALK"
     gene            complement(3349..6276)
                     /locus_tag="VFV93_15475"
     CDS             complement(3349..6276)
                     /locus_tag="VFV93_15475"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5166805.1"
                     /translation="MGVVNQSLLPQLPPSSIVGRNDDQELLRARLDCMVRGRGSLVLI
                     SGEAGIGKTALVASMSLEVQARGIHVTVGHCYDRSATPPYGPWVELANPQHAELLPQL
                     HDEARNDAALEDERVLFNRVVESISNAAHARPLVVVLEDIHWSDPASQDLLRHVARHV
                     DSIPLLLIATYRSDEITEAHPLYALLPLLVRESEATRIDLQRLNTEALASLIAGRYQL
                     LQADQQRLVEHLAARSQGNPLFAHELLRMLEQRGVLRRDGCRWRLDPIQHMQVPPLVR
                     QVIDGRLIHLDPETRSALEVAAVIGQSVPLDLWGRVANLSNEQLDAVVERAVQAHLLE
                     ETLDGRGWHFSHALVHDTFYERVMLPRRRHWHRRIAEELAASDDPDAAVLAYHFDHAG
                     DPCATAWHLRAGERSQQLYATKSAVRHFTAVLDANGATAQQQLIAHRSRGRAHETLGE
                     FDCAEQDFEAAIVLARQVSDRSAEIQILLDAGMLWASRNYDRAGDHYRRALDLARAYA
                     DPSMLARTLNYLGNWYVNGDNSPDAMRHHREALALFEELDDRRGVAETLDLLGMTAMF
                     MGDMSLAVRYYQRAITLAREIGDRKLLSSGIATLTLAPVPGEVTSVIPALAAGIDWRR
                     SANEALAIAREMGWRAGEAYALVLLGSAQTSVGQFEQALRTLSEVISISTEIGHMFWL
                     MCAHESTGVIQYLMLDLEQAQASIEQAVQLAHDLRAGSKIRSTTALLALVMIELGDLE
                     RADTLLRPLRGPVEILASSTVQRDCWLAQSALMLARSQPARALEILDRLIEATPSMTS
                     EHVAPCVARLRARALLALGRPRDAETQLLDGCRSGVTFGTPSQLWRAQLDLGKLYLSQ
                     HRYDEADRELSAARATIDALARNTPEGQRRDTFLQRAHDVFPTGARAPSRATGSPALR
                     LSPREIEVLALLVQGSTDQEIADALAIRHRTVTTHVTNIFNKLGVNTRTAAASLAIRN
                     GLV"
     gene            6493..6678
                     /locus_tag="VFV93_15480"
     CDS             6493..6678
                     /locus_tag="VFV93_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166806.1"
                     /translation="MAMVIDRKPRIRDHGHFWVWFAIAVLAALVLVGVAFVAAGVTAA
                     AAFVAVEAAIAMALGIR"
     gene            7231..9099
                     /locus_tag="VFV93_15485"
     CDS             7231..9099
                     /locus_tag="VFV93_15485"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5166807.1"
                     /translation="MADFPTSLTGSSLSRRDFVRRAAFLGMAFPALGTLLAACGGDDD
                     DDDDDGGDATATTTGAQPEATATIQIIAPVPTSTQASAGSPEATEAAEPTAAATGAAE
                     PTAAATEPSTGAGVKGGSVVFLREGDADNGTYDPVLSDDNDVIWVLFSVYETLVKANP
                     EGSDLDPGLAESWEITEDALTATFNLRAGLKFSDGTDVTTDDIIFSLERARDTETSPW
                     SFSLAQAQQITAPDDATIVINLSEPYMPLFAALAMFNSGIISKAFFEANATESDAEVF
                     GGLAITTGVSCGAGPYAITKWEPTVQTVLTRNEYYWDEGKPYLDEIILRTVPDSNSMI
                     LQLQGGDVDGVIGQNAIPYNRVEELQGDDNLQVIISPASQSSQVTINTNWHGIGNPLE
                     PRMPFADPNFRMALAHAIDHQTLIDTVQFGIAVPSGGLMPVGSMFWNPDVVVPEFNLD
                     KAREFLAASESPDGGSGEIQITTGNAQQEALATALQAMWKEINFEATIGPYPAAVGTQ
                     RRTDGDYDMRIASWTNDMIDPDQILSYYVVPDASANARTGYLDQQAVGMVIQARSETD
                     ENKRRDLYYQVQERWIGGPQFFLYNVPYTVALNKRIKGYHQNPLGPWYFRDIYIEE"
     gene            9346..10287
                     /locus_tag="VFV93_15490"
     CDS             9346..10287
                     /locus_tag="VFV93_15490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166808.1"
                     /translation="MSRGQYILRRLIQMIPVVFGITVITFVLIRAIPGDPAQTMLGEK
                     ATDAQIALYREKMGLNDPVYVQYFYYMRDLVRFDLGTSTQYRVPVKTILWDRLKVSLS
                     VMLMTLVLTTVISIPLGMMAALKKDSFIDNVVRSTLMVSLLMPTFYTGILIIILLSIK
                     VDLFPTAGYGEGFIGHVHHLFLPGLTLALGISPILIRTLRTGILESMTSDYVKTARSK
                     GLAEQTVVTRHVLRNALIPTVTLLGISVGAIIGGTVVTEKVFALPGAGALLVDAINAR
                     DYSTVQAAVMIVAILVVLVNLLTDLVYSFIDPRVRLG"
     gene            10316..11206
                     /locus_tag="VFV93_15495"
     CDS             10316..11206
                     /locus_tag="VFV93_15495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166809.1"
                     /translation="MAPADVVAVDALAAVEHRQRRRRGVGWKLLASRNLVIGGIILTI
                     MVASALLAPWIAPYDPIEQEPRNALQAPSLEHPFGTDNFGRDVYSRVLHGGRIDLKFG
                     FLAVIIPLVAGVVLGSISGYYGSWTDSVLMRVVDIVQAFPFLVLVIFFVAVLGPGLWN
                     MVFAVGVVAWIVYARLIRGSILVEKHKEYVIAARTIGGNDWRVMRQHLFPNVVTPCII
                     YAMADIGAYIGVLATLSFLGLGERPPAPEWGAMIASGRDFMVTDWWICAIPGAAIVIT
                     GIGLSLIGDGLADVLRPGGR"
     gene            11229..>11584
                     /locus_tag="VFV93_15500"
     CDS             11229..>11584
                     /locus_tag="VFV93_15500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5166810.1"
                     /translation="MSVSFDFTGRAVIVTGAARGIGRVMVARFAASGASVLAADRDEE
                     GLAATCRDLDPARVATLVADVSSEAGAESIVAAAVRRFGRLDVCVNNAAVAPHASLFD
                     ERVEVWDTIYAVNTRGT"
BASE COUNT         1992 a         3757 c         3708 g         2127 t
ORIGIN      
        1 cctgttcctg aaccccggca ccgacacgtt ccccgtccag gaggcactcc ggaagctgga
       61 ggtgctgggg cgcaagacgc cgcggactgt cctctgcctg ttcgagatcg tcgcgctggc
      121 ggccgcccac ggctactacg cggcgaccgg ccggccgcag gcggtcctcg tccacgtcga
      181 tgtcggcacc cagaacctcg gcagcatgct ccacaacgcc cagcggggcc gcgcggcggt
      241 cgtcatcgtg gcaggccgcg cgccctacac gaccgacgcg gagcagcgcg gaagccgctc
      301 gtcgtacatc cactggctcc aggagcagcg cgaccagcat gggatcgtcc gcaactacac
      361 caagtgggac tacgagttgc gccgggccga ccaggtcggt gaggtcgtta gccgcgcctt
      421 tcaggtggcc gccagcgacc cacccggccc ggtctacatg acgctgccgc gcgagctgct
      481 gatggagacg gtcggcgagg tctcgatcca ccagcccgga cgcttcccgc cggcccggct
      541 cggcgccggc gatcccgacg cgctgcgcga ggtcgcccgc cggctggtgg ccagcgagcg
      601 cccgctcgtg ctgaccggca acagcggccg gactgaagcg ggctaccacg gcctgatccg
      661 gttggccgag ctgctggcgc tgccggtcgt cgagtggcgc aaccgggcga acttccccgg
      721 cgatcacccg ctccacctcg gctacgcggt tcgcccgctg ctggacgatg ctgacgtcgt
      781 cctggcgctc gaccacgatt acccctacat cccgacgacg gcccagccgg ccgagagcgc
      841 cttcatcgtc cagctcgatc tcgacccggt caaggagcag gtgccactct ggaccttccc
      901 ggtcgatctg ccgatccggg gtgatacatc ccgcgcgctc gacctgatcg ccgacgaggc
      961 cgaacggttg ctgaccggcg cccagcgcga gcggatcgaa gcccgccgca acgcgctgac
     1021 gatccagcac gaggagcttc gcgagacatg gcgggccgag gcgttggccg gtgaggcgac
     1081 gctgccgatc acgattccct ggctggcccg ctgcctgcgc gagctccagg acgacgctcc
     1141 cgacctgatc gtggtcgatg agatggtgac cagtaacgaa acggtctgga agcaattccc
     1201 cgttcgcacg cctggtagct ggtacggcag tggcggctcc ggcctcggct ggggacttgg
     1261 cgcatcgctg ggcgtcaagc tggcgcaccc cgaccggccc gtcctcgcgc tggttggcga
     1321 cggctcgttc atgttcggga caccaacagc cgccctctgg gcgcagcaga cgcagcaggc
     1381 gccggtcctg accgtcatcc tcaacaacac ctgctacaac gcgaccaagc gcccgctggt
     1441 cgccaactac ccggccggct actcggtccg cgaggacagc ttcatcggcg tcgacctgac
     1501 cccggcccct cgctacgaca agctggccga cgtcgtcggc gcctacggcg aacgggtcga
     1561 atcccccgct gatgtcctgc ccgccctcca gcgcgccctc gaacgcacgc gctcgggcca
     1621 gtccgtcgtg ctggacgtgc agttggcgca tccgtgatgc ctggcgtgtg atgcgtcgtc
     1681 gcggtttcgc cgtcggacac gacgcatcac acgccctgcg ggtcacttca acgccgggga
     1741 gatgttgttg ttcatccgga acaggttggt tggatcatag cgcgtcttga tgtcgacgag
     1801 gccatcgtag ttagtgccat agctggcgcg aacgagcgcc tctttctcct cgccgaagcc
     1861 ggcgaagttc aggtacagcc cgccttgcga gaacgcgtga gcagctctcc atgtgtctct
     1921 ggtccactgg atacagcggt ccgtatcagc ctcgttgttc caggtagtat ccaggctaag
     1981 caggtagggg gcttcacggc tgccgaaggc ggtttctgag gcggccgtcc gtgtcatctg
     2041 tccgccgagg tgccagagtg ccacggctgt gatcggcgtt ggccggttgg ctccaatctg
     2101 tgtgagcgca gcgatctccg gctcctccag cttgttcagg tagagcgatt tccagtagta
     2161 gaaccagccc ttggggaaga gcgggtcgaa actgctctgg agctccgtgt agggtacggt
     2221 gccgctcatg tccagtactg gctctgccag gtgtcgcagc ggcacgagtg ccgcctcgcc
     2281 ctcgtccacc gcgccggtat ggagcgccgc cactagcagc acgggtcggt ggtgcatttc
     2341 ggcagggatg aagtcggcag ggggaaggca ccagaagacg gccgacccgc tgatctcttc
     2401 tggcgcagcg tccatgaagc cgcgccagcc tgccagcacc cgcggcccgt cctctggtgc
     2461 atatgctgtt gccgccagcg cgaccatcgg accaaccggg tgcatcgcga actcgaacga
     2521 cgtgacgaca ccgaagttgc tgccggcccc gcgcactgcc cagaacaggt ctcggttctc
     2581 ctcggagttg gctgtccgga gttggccatc ggcggtgacg atgtcgaccg agatcaggct
     2641 gtcgatcgac aggccgtact tgcgccgcac ccagcccaac ccgccgtgca gtgtcagacc
     2701 gccgatgccg gtcgatgaca ccacgcctcc gggaacggcg aggccgaaca tctgcgtctc
     2761 gtggtcgaca tcaccccagg tcgcgccgcc ttccgcgcgg accgtaccgg ctttgagatc
     2821 gacacgaacc ccacgcatcc gcgagagatc gatcacgatc ccgccatcgt tgacggcgtt
     2881 gccggcgacg ttgtggccac caccgcgaat cgacagcacc aggttgttgt cgcgagcgac
     2941 gttgaccgcc gagatcacgt cggccacgcc cgtgcagcgg gcgatcaacg ccgggcggcg
     3001 gtcgatcagt ccattgtgga cctcacgtgc gtcgtcgtag gctgggtcgc ccggctggag
     3061 cagctcgcct cgatgactcg ccgagagtga ttcgatgacc tgcgcggtca ggccaggacg
     3121 ggtcgtctcg atcgttgcca tgcgcgtgtc ctctcatcgt ctgcgctccg ccacgaggag
     3181 cgcatcgtcg gtgacggcat gcgggtcgaa taatggtccg gtttcgatga ttggcatcct
     3241 gagggattgc ggattatctg acatcgtcaa gattacggag ggcaactggc gatgttcggc
     3301 tacgtattcg gctacgtatc ggcgtatcac ggacgcccgt cacccggatc agaccaggcc
     3361 gttgcggatg gccaggctgg cagcggcggt gcgggtgttg acgccgagct tgttgaagat
     3421 gttggtcacg tgggtcgtta ccgtccggtg acggatcgcc agcgcgtcgg cgatctcctg
     3481 gtcggtcgat ccctggacca gcagcgcgag tacctcgatc tctcgagggc tgagccggag
     3541 tgccggcgaa cccgtcgccc gggatggagc ccgcgcgcct gtcggaaata cgtcgtgcgc
     3601 tcgctggagg aacgtgtcgc gtcgttggcc ttccggcgtg tttcgagcga gtgcgtcgat
     3661 cgtcgcgcgg gccgcgctca gttcgcggtc cgcttcgtcg tagcggtgct gggagaggta
     3721 gagcttgcct agatcaagtt gtgctcgcca gagctggctg ggagtcccaa acgtgacacc
     3781 ggagcgacag ccgtccagca gttgtgtctc ggcatcacgt ggcctgccta gcgccagcag
     3841 cgccctcgcc cgcagccggg cgacgcaggg ggcgacatgc tcactcgtca tcgacggcgt
     3901 cgcctcgatg agccggtcga ggatctcgag cgccctggcc ggctgcgacc gcgccagcat
     3961 cagcgccgac tgagccagcc agcagtcgcg ctggaccgtc gacgacgcaa ggatctcaac
     4021 cggcccacgc agcggtcgca ggagggtgtc agcacgctca agatcgccga gctcaatcat
     4081 gaccaacgcc agcaacgccg tcgtcgacct gatcttggat cccgcgcgca gatcgtgggc
     4141 gagctgaacc gcttgctcga tcgatgcctg tgcctgttcc aggtccagca tgagatactg
     4201 gatgactccg gtcgactcat gggcgcacat cagccagaac atgtgcccga tctcggtcga
     4261 gatcgagatc acttcgctga gggtgcgcaa cgcctgctcg aactggccga cactggtctg
     4321 agcgctaccg agcaacacga gggcatacgc ttcaccggca cgccagccca tctcacgcgc
     4381 gatcgcgagc gcctcgttgg cgctccggcg ccagtcgatc cctgcagcga gcgcgggtat
     4441 gacgctcgtg acctcgcctg gaaccggtgc cagtgtgagc gtcgcgatgc cggaactgag
     4501 aagcttccgg tctccaatct cccgtgcgag cgtgatggca cgctggtaat agcgcaccgc
     4561 cagtgacatg tcgcccatga acatcgccgt catgccgagc aggtcgagtg tctcagcgac
     4621 gccacgccga tcatccagtt cctcaaagag tgccaacgcc tcgcgatggt gacgcatggc
     4681 gtccgggctg ttgtcgccgt tgacgtacca gttgccgaga tagttcagcg tccgtgccag
     4741 catcgacggg tccgcgtacg ctcgtgccag gtcgagtgcc cgccggtagt gatctccggc
     4801 ccggtcgtag ttgcggcttg cccagagcat tcccgcgtcg aggagaatct ggatctcggc
     4861 tgagcggtcg gacacctgac gtgccaggac gatggccgcc tcgaaatcct gctcggcgca
     4921 gtcgaactcg ccaagcgttt cgtgtgcgcg gccccgagag cgatgtgcga tcagttgttg
     4981 ctgggctgtt gccccgtttg catcgaggac ggcggtgaaa tgcctcacgg ccgacttcgt
     5041 tgcgtagagt tgctgggagc gctcgccggc gcgcagatgc cacgcggtcg cgcagggatc
     5101 gccggcgtga tcgaagtgat aggccagtac cgcggcgtca gggtcatcac tggcggcaag
     5161 ctcctcagca atgcgccggt gccagtggcg gcggcgtggc agcatcaccc gctcgtagaa
     5221 cgtgtcgtgg acgagggcgt ggctgaaatg ccagccgcgt ccgtccaacg tctcctctag
     5281 cagatgagcc tggacagccc gttccaccac agcgtcgagt tgctcgttgg agagattggc
     5341 gacgcgaccc cagaggtcga gcgggactga ctgtccgatt acggcagcaa cctccaatgc
     5401 cgaacgagtc tctgggtcga ggtggatcag ccgcccatcg atgacctgac ggacgagcgg
     5461 tggcacctgc atatgctgga taggatcaag ccgccagcga caaccatctc gacgcaggac
     5521 acctcgctgc tccagcattc gcaggagctc gtgtgcgaag agcggattcc cctgcgaacg
     5581 tgccgcgaga tgctcgacca gtcgctgctg gtcagcctgt agaagctggt atctcccggc
     5641 gatcagactc gccagcgctt ccgtgttgag tcgctgcagg tcgatgcgag tcgcttcgct
     5701 ctcccgcacg agcagaggca ggagtgcata tagcggatgc gcttcggtga tctcatcgct
     5761 ccggtaggtc gcgatgagga gcagtgggat gctgtccacg tgccgcgcaa cgtgacgcag
     5821 cagatcctga ctggctggat ccgaccaatg gatgtcttcg agcacgacga cgagcggacg
     5881 ggcgtgagcg gcgtttgaga tggattcgac gacccggttg aagaggacac gttcgtcttc
     5941 gagagcagca tcgttccgcg cttcgtcgtg gagttgggga agcagctctg cgtgctgcgg
     6001 gttggcgagt tccacccatg ggccgtaagg tggtgtcgcc gagcggtcgt aacagtgacc
     6061 gaccgtgacg tggatcccgc gtgcctggac ttccaagctc atggacgcta cgagagccgt
     6121 cttgccgatg ccggcttcgc cactgatcag gacgagactg ccccggcccc ttaccatgca
     6181 gtcgagtcgt gcgcgcaaga gttcctggtc gtcgttgcgg ccaacgatcg atgaaggagg
     6241 caactgcggc agcagggatt ggttcacgac gcccatgaca gcgacccctc gccccgacca
     6301 gtgagtattt cgatggcaac aggatactcg atgattgcag ccctgtcccc tcgtacaggt
     6361 ccaaattcgg ccttgcagcc gatgggctga ggggtggtcg ctcctacaat tggggtcatg
     6421 cggtcgaggc tgaccgcgac gccgcgatga tgcggaaacg agattgcagc gccgcaagag
     6481 aggagcacga cgatggccat ggtaatcgat cgcaaaccac ggattcgcga tcacggacac
     6541 ttctgggtct ggttcgctat cgccgtcctc gccgcgctcg ttctggttgg cgttgccttt
     6601 gttgcggccg gcgtcaccgc tgccgccgcc ttcgtcgccg tcgaagccgc gatcgcgatg
     6661 gctctcggca tccgctgagc catcccccca gattagacgc tgacgcgccg gcccagccgg
     6721 cgcgttcgtg cttcccgccc gctatggcgt cgagttgtgg ctttcgcact cgctacgcat
     6781 acaacaaatc caagtccaat atactgcatc aagtcgcaaa ggcactgcgt caccccgtca
     6841 gattgttgac atttcggcgg ttcaatcgta ctctgcgatc tacagtggtt ccctgaacac
     6901 aggttcgcgt ggttgaggac ggcgccgatg ggttgtccta atgcacaccg ttagcgtcga
     6961 tggtgccggc ctccggcttg ccgatgtgcc gcggccaggt ctccgtcgcc atgccatcac
     7021 gttcacttga ggggccaacg catcccgggc catcgcccag cgatggcttg ccgccacgat
     7081 cgattcggtc acaactccgt gcttccctga tgtcgttgct ggtctccttg cctgcaaggg
     7141 acatcgtcga ggttgctcgg aggaagtgat cgaccggtgg gatgcgtctg tacgtgtagc
     7201 gatggtcgag aggtagagag gacgctcaac gtggctgact tcccaacatc cctgaccggt
     7261 agttcgctca gccggcgtga cttcgtcagg cgcgccgcct tcctggggat ggccttcccg
     7321 gcgctcggca cgctcctggc tgcctgcggc ggcgatgatg acgatgacga tgatgacggc
     7381 ggcgacgcaa ccgcgacgac caccggagcc caaccggaag caaccgcaac catccagatt
     7441 atcgctccgg tcccaacgtc gacccaggcg agcgccggct cgccggaggc aactgaggct
     7501 gcggagccga cggctgccgc aaccggggcc gcagaaccaa cggccgccgc aaccgagccg
     7561 tcgaccgggg ccggcgtaaa gggaggcagc gtcgtcttcc tgcgcgaggg tgacgccgac
     7621 aacggcacct acgacccggt tctcagcgac gacaacgacg tgatctgggt tctcttcagt
     7681 gtctacgaga cgctggtgaa ggccaacccc gaaggctccg acctcgatcc gggtctggcc
     7741 gagtcgtggg agatcaccga ggatgcgttg accgcgacgt tcaaccttcg cgcgggcctc
     7801 aagttttccg acggcaccga cgtaaccacc gacgacatca tcttctcgct cgagcgcgcc
     7861 cgcgacaccg agacgagtcc ctggtcgttc tccctggccc aggctcagca gatcaccgcg
     7921 cccgacgacg cgacgatcgt gatcaacctg tctgagccgt acatgcccct cttcgccgcg
     7981 ctggcgatgt tcaactccgg catcatctcg aaggcgttct tcgaggccaa cgcgaccgag
     8041 tccgacgcgg aggtgtttgg ggggttagct atcaccaccg gagtctcttg cggcgccggt
     8101 ccatacgcaa tcaccaagtg ggagccgacc gtccagacgg tcctcacgcg caacgagtac
     8161 tactgggatg agggcaagcc ctacctcgac gagatcattc tgcggaccgt gccggactcc
     8221 aacagcatga tcctgcaact gcagggcggc gatgttgacg gcgtgatcgg ccagaatgct
     8281 atcccgtaca accgcgtcga agagctgcaa ggggatgaca acctgcaggt gatcatctcc
     8341 ccggcttctc aaagctccca ggtcacgatc aacaccaatt ggcacggaat cggcaatccg
     8401 ctcgagccgc gcatgccgtt cgctgacccc aactttcgca tggcgttggc gcacgcgatc
     8461 gaccaccaga cgttgatcga cacggtccag ttcggcatcg ccgtgccgtc cggcggcctg
     8521 atgccggtgg ggtcaatgtt ctggaacccc gatgtcgtgg tgccagagtt caacctcgac
     8581 aaggcgcgcg agttcctggc ggcgtctgaa tctccggacg gtggctcagg cgagatccag
     8641 atcaccaccg gcaacgcgca gcaggaggcg ctcgcgactg cgctgcaggc gatgtggaaa
     8701 gagatcaact tcgaagcgac catcgggccg tatccggccg cggtggggac ccagcgccga
     8761 accgatggtg actacgacat gcgcatcgcc agctggacga acgacatgat cgacccggac
     8821 cagatcctgt cgtactacgt ggttccggat gcgtcggcca acgcccgcac cggctacctc
     8881 gaccagcaag cggtgggcat ggtcatccag gctcgctccg aaaccgacga gaacaagcga
     8941 cgcgatctct attaccaggt ccaggagcgc tggataggcg ggccacagtt cttcctgtac
     9001 aacgtcccgt ataccgtcgc gctcaacaag cgcatcaagg gctaccacca gaatccactc
     9061 ggcccgtggt acttccggga catctacatc gaggagtagc gcggtcagtc cggcagtcag
     9121 caactaaatg aacagcgtca acaaggagtg tccacccaac ttcctgtcat cctgagccgc
     9181 agcgaaggat ctctcctgta caagccggtc atttcaacgc ggagagatcc ttcgctgcgg
     9241 ctcaggatga caggggggtg ctggtgaagc cctcacaaac gccgtccatc tggatactga
     9301 ccgcgtgacg acctgactgg ctgaccggct acaaaggaac tgcacatgtc acgagggcaa
     9361 tacatcctac gacggctcat ccagatgatc cccgtcgtct tcggcattac ggtcatcacc
     9421 ttcgtgttga tccgcgctat cccaggtgat ccggcccaga ccatgctcgg cgagaaggcg
     9481 accgacgcac agatcgcgtt gtaccgcgag aagatggggc tcaacgaccc agtctatgtg
     9541 cagtacttct actacatgcg cgacctcgta cggtttgatc tgggcacgtc gacccagtac
     9601 cgcgtgccgg tcaaaacgat cctctgggac cgcttgaaag tgtcgctatc ggtgatgctg
     9661 atgacgctgg tgctgacgac cgtgatctcg attccgctcg gcatgatggc ggcgctgaag
     9721 aaggacagtt tcatcgacaa tgtcgtgcgc tcgaccctga tggtgtcgct gctgatgccg
     9781 accttctaca ccggcatcct gatcatcatt ctcctgagca tcaaggtgga tctcttcccg
     9841 actgccggct atggcgaggg gttcatcggc cacgtgcatc atctgttcct gccggggctg
     9901 acgctcgcgc tcggcatctc gccgatcctg atccggaccc tgcgcacggg tatcctggag
     9961 tcgatgacct ctgactacgt caagacggcg cgctccaagg gactggcgga acagacggtg
    10021 gtcacgaggc acgtgctccg caacgcgctg atcccgaccg tgacgctgct tggtatcagt
    10081 gtcggcgcga tcatcggcgg gacggtcgtc actgagaagg tgtttgcctt gcctggcgcg
    10141 ggcgcgctgc tggtcgacgc gatcaatgcg cgcgactact ccaccgttca ggcggcggtg
    10201 atgatcgtcg caatacttgt ggtgctggtc aatctgctga ccgaccttgt ctactcgttc
    10261 atcgatccgc gcgtgcgatt ggggtagcag gatggccgta cgaagagcca cggacgtggc
    10321 gcccgccgat gtcgttgctg tagatgcgct ggcggccgtt gagcaccgac aacgacggcg
    10381 gcgaggtgtc ggctggaagc tgctggcaag tcgtaatctg gtcatcggcg ggatcatcct
    10441 gaccataatg gtggcctccg cattactggc gccctggata gccccctacg atccgatcga
    10501 acaggaaccc cgcaacgcgc ttcaggcgcc gagtctcgag catccattcg gcaccgacaa
    10561 cttcggccgc gatgtctact cgcgggtcct gcatggcggg cggatcgacc tgaagttcgg
    10621 cttcctcgcc gtgatcatcc cactggtcgc cggcgtcgtg ctcggctcca tctccggcta
    10681 ctacggcagc tggacggatt ccgtcctgat gcgcgtcgtt gatatcgtcc aggcttttcc
    10741 gttcctggtg ctggtgatct tcttcgtggc cgtgctgggt ccgggcttgt ggaacatggt
    10801 ctttgcggtc ggagtggtcg catggatcgt ctatgcccgc ctgatccgcg gatcaatcct
    10861 ggtcgagaaa cataaggaat atgtcatcgc cgcccggacg atcggcggca acgactggcg
    10921 tgtgatgcgc cagcacctgt tcccaaacgt cgtcacgccc tgcatcatct acgcgatggc
    10981 cgacatcggt gcctacatcg gcgtgctggc aacactcagc ttccttggcc tcggcgagcg
    11041 tccaccggct cccgagtggg gcgcgatgat cgcctcggga cgggatttca tggtcaccga
    11101 ctggtggatc tgtgccatcc ccggagcggc gatcgtgatc accggcatcg gcctcagcct
    11161 gatcggcgac ggccttgccg atgtgctgcg gccaggcgga cgctaacgcc tttgaattgg
    11221 agcattgcat gagtgtgtcg tttgacttca ccggccgggc tgtcatcgtc accggcgcgg
    11281 cgcgggggat cgggcgggtg atggtcgcgc gttttgcggc gtccggtgcg tcggtcctcg
    11341 ctgccgaccg cgacgaggag ggattggcag cgacgtgccg cgacctcgat ccggcccgtg
    11401 tcgccacact cgttgctgat gtcagttccg aggccggtgc ggagagcatc gtcgcggcgg
    11461 ctgtccggcg ctttggccgg ctcgatgtct gcgtcaacaa cgcggccgtt gcgccgcacg
    11521 cgagtctgtt cgacgaacgg gttgaggtct gggacacgat ctacgccgtc aacacgcgcg
    11581 gcac
//
LOCUS       DASUGJ010000682         8220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1361609, whole genome shotgun sequence.
ACCESSION   DASUGJ010000682 DASUGJ010000000
VERSION     DASUGJ010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361609"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2019)
                     /locus_tag="VFV93_15505"
     CDS             complement(<1..2019)
                     /locus_tag="VFV93_15505"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEX5166811.1"
                     /translation="MYAILVVIMLLGSAVVPQFGLRPSNVSFQASQSGTSTTLTAVAD
                     SYVSAKEPDRNYGKDTLLHADRSPEVRTLLRFDVPADSGNIQRATLRVYVEKNHPTGF
                     NVQTAGSNWDESSVTAKSAPAVNAQTIATSPRLTKGSWVTVDVTTAVTAAGPLSLVLS
                     STNDADVAVTSREKAARAPQLLLETGAATIPAPDPTPSPTPSPTPSPTPGAQPSPTPA
                     PDVPSAPEAPTAPEPVAPKPGSGPNAVAPATMNLTSTFNSIGVELFFSGDDNNNATAN
                     LDFRRTGESSWRQGLPLWGTYANPRAFYGSALLLDAGKTYEIRVSFSDPDGVNGNFTV
                     TGSIATRAEDIAGANQLTPTHFVSAQGNDANTGTSPNSAWKTLEKAFKSAPAGAVVRV
                     AAGSYAPPTTNRKLPITLVAENPAVDDGQNIINAGKHSVIEPQTITERNSGTWTKVNL
                     KGPATGQTYSVWKWTGSPVNNATVLAWSNSRTDEPQRVAYWNRRDGKFDGYTMQTPEG
                     WAEVLYRNETYNFGFASFGNDVYLRLPGDKNPNDVVMTLHNTWMSGDTGRVVLSGSNV
                     RMSGFELRLVDAMMRSPSVNGVIDHNLFYMSTVYYKGEKGTPSSYPTNQVVQYNRMID
                     TNLWSRDGGDNVIPWLFVKQSLKLNGKMTDWRRTGEGAELHAIHGRGGA"
     regulatory      complement(2111..2186)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2508..2780)
                     /locus_tag="VFV93_15510"
     CDS             complement(2508..2780)
                     /locus_tag="VFV93_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166812.1"
                     /translation="MLTDQIVHQPMQQTAPQEPSSATIVAAAYCEDGWEIEVEDATGI
                     RLTYWVGGQSAAPAVGATVLFYHGSADHRLCGMEIDGHRLFDTCTT"
     gene            complement(2915..3802)
                     /locus_tag="VFV93_15515"
     CDS             complement(2915..3802)
                     /locus_tag="VFV93_15515"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5166813.1"
                     /translation="MQRFLLLGLFGLVAQFIDGSLGMAYGVTATTMLLAGGVAPAMAS
                     ASVHLAEVGTTLASGLSHWKLGNTEWRAVGWMAVPGGIGAFTGAILLTSISAEVASRW
                     IAAFLICLGVYILLRFTFGAYRLRSGRRIRGRYLAPLGLGAGFLDAAGGGGWGPIATP
                     TLLASGRMEPRKVVGTVDTSEFLVSLGASAGFLFGLNRAEIPFTTVAALLLGGVIAAP
                     FAAWLVRIMHPRLLGAGVGGLIIFTNSRTLLKAAGYDADARYLLLTCLALLWLGAVAL
                     AAQAVLGERAQRQASATGD"
     gene            complement(4109..4819)
                     /gene="queC"
                     /locus_tag="VFV93_15520"
     CDS             complement(4109..4819)
                     /gene="queC"
                     /locus_tag="VFV93_15520"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX5166814.1"
                     /translation="MPAELDPTSPAVVLLSGGVDSTTCLAIAKSEGFVPHALSFRYGQ
                     RHLVELEAARRVALALGAREHVIAEIDLRVFGGSALTAEIDVPKDRPLGEMDTGVPIT
                     YVPARNTIFLSFALAWAEVLGAQDIYIGVTAIDYSGYPDCRPEYIHAYERMANLATAA
                     GVDGRQRLTIHTPLIDLSKAQIIQRGLALGVDYGLTLSCYDPRPDGTPCRHCDSCLLR
                     AKGFAEAGLADPALSPTV"
     gene            complement(5018..7387)
                     /locus_tag="VFV93_15525"
     CDS             complement(5018..7387)
                     /locus_tag="VFV93_15525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5166815.1"
                     /translation="MTTVTIGIHVHAEARRLAETLASVRANTGPPHEVVLLPDGPEPD
                     VVDYLATLPDLHQLGTQAALGPPACFNRLALAGDADVLVLLESGAVVGPGWLELLLAA
                     FEADPTTGIVGPSTNRAWNEQAVVRQSGSTLVEVAAIVGEVRKRFGAGSRTLEPLHSL
                     ADFCYVVRREVVERIGAADEGYGLGPCWEMDYNIRAVRAGFRVAWVGGAYVQRAPFTP
                     RRRRAERLLLDASRRRYQDAFCGLRLTGSASGYQPHCRGEACEHFAPPDLIRRAIPLR
                     PSALPEPPVAPASPPPVVAPPEPATHLVSCIMPTGNRARFALQAIALFQRQDYADREL
                     IVVDDGDDDLEQQLPADPRIRYLRVPRGLTIGAKRNRACDLARGTFIAHWDDDDWYAP
                     DRLRRQLAPLLAGEAEISGLRGGVILDVPRWAFWTTTPELHRRLFVGDVHGGTLVYQR
                     RLWEQVAKYPNRSLAEDAIFLRRCRQRRARLAVLENNGQFVYLRHASNSWSFACGSYL
                     DPAGWLPAEEPDYLRADRAFLTSLSPAMSAPVPAGPIIVVTPDTTPLVSCIMPTADRR
                     EFVRRSIADFQRQDYPNRELIVVDDGEDAVADLLDGDPRIRYLRLSGKRTLGEKRNIA
                     CREAGGDYLLHWDDDDWMAARRIRVQIGALRESQADVCGLDRLLYHDPAAGLAWEYVY
                     PGRRGRWVAGNTLCYTRALWLRNPFINAQVGEDTRFLRSRVPKKIVPLADTTICIGRV
                     HPGNTSPKHTTGSPWRPVPLSTIEELLRAESVECSPSSVEAVAPSRPAP"
     gene            complement(7384..>8220)
                     /locus_tag="VFV93_15530"
     CDS             complement(7384..>8220)
                     /locus_tag="VFV93_15530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5166816.1"
                     /translation="ADLTRSAGRLTARWHGHDANVLHFNGLGRHKYPEWRGLYGGVAQ
                     PLAGAGSGDGYGQFLDALRAWLGEQGQEALRWSFYGTANAASASVHDPATFPLLALLH
                     YLVRSNGCVRVLETGTARGISAACLASAVAHRAGGRVVSFDPFDQPGRCELWDMLPPA
                     MSACLEQRPLDSVAGMAAALDDGERYDAALLDSLHEAEHVWNEFELATKLVCPGGLIL
                     IHDVCLATGSVDRAVARIEAAGYGVARLWAAECGEPEGDRLGLAVIENRRRSRSNCER
                     FA"
BASE COUNT         1371 a         2752 c         2782 g         1315 t
ORIGIN      
        1 cgcgccgccg cggccgtgaa tcgcgtgcag ctcggcgcct tcgccggtcc gccgccagtc
       61 ggtcatcttg ccgttgagct tcaggctctg cttgacgaac agccacggaa tgacgttgtc
      121 gccgccatcg cgcgaccaga ggttggtgtc gatcatccgg ttgtactgga cgacctggtt
      181 ggtgggatag ctggatggcg tgcccttctc gcccttgtag tagacggtgc tcatgtagaa
      241 caggttgtgg tcgatgacgc cgttgacgga cggcgagcgc atcatcgcgt caaccagccg
      301 cagctcgaag cccgacatac gcacgttcga tcctgacagc accaccctgc cggtgtcgcc
      361 ggacatccac gtgttgtgca gcgtcatgac gacatcgttc gggttcttgt cgcccggcag
      421 ccgcaggtag acatcgttac cgaatgaggc gaagccgaag ttgtaggtct cgttgcgata
      481 gagcacctca gcccagcctt cgggcgtctg catcgtgtag ccatcgaact tgccgtcgcg
      541 gcggttccag taggcgacgc gctgcggctc gtcggtccgc gaattcgacc aggccaggac
      601 ggtcgcgttg ttgaccgggc tgccggtcca cttccagacg gagtaggtct ggccggtggc
      661 cggacccttc aaattgactt tcgtccaggt gccgctgttg cgctcggtga tcgtctgcgg
      721 ctcgatgacg ctgtgcttgc cggcgttgat gatgttctgg ccatcatcga cggccggatt
      781 ctcggccacc agcgtgatcg gcagcttgcg gttcgtggtc ggcggcgcgt acgaccccgc
      841 cgcgacgcgg acaaccgccc cggccggcgc cgacttgaac gccttctcaa gcgtcttcca
      901 ggcgctgttc gggctggtgc cggtgttggc gtcgttgccc tgcgcgctga cgaagtgcgt
      961 cggcgtcaac tggttcgcgc cggcgatgtc ctcggcccgc gtcgcgatgc tgccggtcac
     1021 ggtgaagttg ccgttgacgc cgtcaggatc gctgaacgag acgcggatct cgtaggtctt
     1081 gccggcatcc agcaacaacg ccgagccgta gaaggcacgc gggttcgcgt aggtgcccca
     1141 gagcggcagc ccctgtcgcc agctcgattc gcccgtgcgg cggaagtcca ggttggccgt
     1201 cgcgttgttg ttgtcgtcgc cgctgaagaa cagctcgacg ccgatgctgt tgaaggtgct
     1261 ggtcaggttc atcgtcgccg gagcaacggc gttcgggccg gagccgggct tcggcgcgac
     1321 cggttccgga gcagtcggcg cttccggagc agatggcaca tccggtgccg gcgtcggcga
     1381 tggttgcgcg ccgggtgtcg gtgacggagt tggcgaaggt gtcggcgacg gcgtcggatc
     1441 gggcgccggt atggtcgccg cgccggtctc gagaagcagc tgcggggcac gggccgcctt
     1501 ctcgcggctc gtgacggcca cgtcggcgtc gttggtgctc gacagcacca ggctgagcgg
     1561 gccggcagcg gtgacggccg tggtcacatc gaccgtgacc cacgaaccct tcgtcagccg
     1621 tggcgaggtc gcgatcgtct gcgcgttgac cgccggagcg ctctttgccg ttacggacga
     1681 ctcatcccag ttcgagccgg ctgtctggac gttgaagccg gtcggatggt tcttctcaac
     1741 gtagacgcgt aatgtcgcgc gctgaatgtt cccgctatcg gccggaacat cgaagcgcag
     1801 gagggtgcga acctccggcg agcggtcggc gtgcagcaac gtgtccttgc cgtagttcct
     1861 gtccggttcc ttggcggaga catagctgtc cgcgacggcg gtcagcgtcg ttgacgttcc
     1921 tgattgcgat gcttgaaatg agacgtttga cggccgcaaa ccaaactggg gaacgacggc
     1981 gctacccaac aacattatga caacgagtat cgcgtacatg ggtccctttc ttcgaacata
     2041 ccagggaccc gattcacgcc acgtttcgcc acaatgcctg accatgagga tgtgtcacaa
     2101 atctcgtgac ttcggcaacc tggctgtctc cggagagatc catggctttg cgtccccacc
     2161 tcgcgacggg tttgccgttt cgggtccacc gactatcgac ttgcgaagag ggtaacaaac
     2221 catgtcgagg tcgcgcatcc cacgaaagtc cggacacgga gcgccgaagg tcatcccaaa
     2281 ttgtggcata tggtaacgcg aagcgtgaat tgcctgccgc caagttgcgc ggcacttggt
     2341 caacattcgc gggtggttga ggggtttgcc gaatgtgaat cgcgcctgta cgacctgatt
     2401 cgggtatgtg cgtctgtgtg cgtcagccat tcaacctctg caatggccgg gccgcaaggg
     2461 tgacagacag gcaccgatca ggtcgatcgc ggagcgtggc gcgtcaatca ggtcgtgcag
     2521 gtatcgaaga ggcgatgccc gtctatctcc atgccgcaca gcctgtgatc ggcactgcca
     2581 tggtaaaaga ggacggtcgc gccgaccgcc ggcgccgccg attggccgcc gacccagtag
     2641 gtcagccgga tgccggttgc gtcctcgacc tcgatctccc agccatcctc acagtaggct
     2701 gccgccacga tcgtcgcgct ggacggctcc tgcggcgctg tctgctgcat cggctggtgg
     2761 acgatctgat cggtcaacat ctgggcgctc ccattcgagc aggtgagaca acagacagaa
     2821 ctaccggcct tcggagccta gcccgagcgg gtcgtggtcc ggttgatcgt gcgttgagat
     2881 tggatcacgc tgagttgatg ggtacggggg cacgtcaatc accggtcgcg ctcgcctggc
     2941 gctgtgcacg ctcgccgagg acggcctgcg ccgccagcgc aaccgcgccg agccagagca
     3001 gcgccaggca ggtcagcaac aggtagcggg cgtcagcgtc gtagccggcg gctttcagca
     3061 gcgtcctgct gttggtgaag atgatcagcc cgccgacgcc ggccccgagc aggcgcgggt
     3121 gcatgatccg aacgagccag gccgcgaacg gggccgcgat cacaccaccg agcagcagcg
     3181 cggcgacggt cgtgaacggg atctcggcgc ggttcagccc gaacagaaac ccggcactgg
     3241 cgccaagcga caccaggaac tcgctggtgt cgaccgtccc gaccacctta cgcggctcca
     3301 tccggccgga tgcaagcagc gtcggcgttg caatcggccc ccagccgccc ccaccggctg
     3361 cgtccagaaa gcccgcgccg aggccgagcg gcgccaggta gcgaccgcga atgcgccgcc
     3421 cgctgcggag gcggtaggca ccgaacgtga agcgcagcag aatgtagacg ccgaggcaga
     3481 tcaggaaagc ggcgatccag cgcgacgcga cctcagccga gatcgatgtc agcagaatcg
     3541 cacccgtgaa cgctccgatc ccgcccggaa ctgccatcca gccaaccgcg cgccattcgg
     3601 tgttgccaag cttccagtgc gacagaccag aggccagcgt tgtgccgacc tcggccaggt
     3661 ggacgctggc ggaggccatc gccggcgcga caccaccggc cagcagcatc gtcgtcgccg
     3721 tcacgccgta ggccatcccc agtgaaccgt cgataaactg cgcgaccagg ccgaacagac
     3781 ccagcagcag aaaccgctgc atgaggcaga acccctttcg ccgcagcgac cgtcacggcc
     3841 gtgcagtctg aacgcacgtc atatctttcc tacaggaagt ctggacaacc ccggatgagt
     3901 tcaggcacgg atcgcgttga attcgcgttg agggctgccc tgtcgccgcc cgcctcgtgt
     3961 aggggccgat cctgtatcgg cccgcgccgt gaggcgcccg cgtaatgccg tgcatggttt
     4021 cccaacgggc gcaggcgttg gggatcctcg tcacacacgc acgaccggcg aaggcgcctc
     4081 acggcgcggg ccgatacggg atcggcccct acacagtggg ggacagggcg ggatcggcga
     4141 gtcccgcctc agcgaaccct ttcgcgcgca gcaggcatga atcacaatgc cggcaggggg
     4201 tgccgtccgg tctgggatcg tagcaggaga gcgtcagccc gtagtcaacg cccagcgcaa
     4261 gcccacgctg gataatctgc gctttcgaga ggtcgatcag cggtgtgtgg atcgtcaatc
     4321 gctggcggcc atcgacgccg gccgccgtcg ccaggttggc catcctctcg taggcgtgga
     4381 tgtactccgg ccggcagtcg gggtagccgg agtagtcgat cgccgtcacg ccgatgtaga
     4441 tgtcctgcgc gcccagcacc tcggcccagg ccagcgcgaa cgagaggaag atcgtgttgc
     4501 gcgccggcac gtaggtgatc gggacgccgg tgtccatctc gcccagtggc cggtccttcg
     4561 ggacatcgat ctcggccgtc agcgccgacc cgccgaacac ccgcaggtcg atctcggcaa
     4621 tgacatgctc ccgtgcgccg agcgccagcg ctacccgccg ggctgcctcc agctcgacca
     4681 ggtgccgctg gccgtagcgg aacgacagcg catggggcac gaacccttcc gacttcgcaa
     4741 tcgcgaggca ggtcgtcgag tcaacaccgc cggaaagcag cacgacggcg ggcgacgttg
     4801 gatccagttc ggcaggcatc ggcgactcca gtaagcagcg actgatagca ggccagtagt
     4861 ctacgaacgg accagactca gtgctagacg accgtcctcg gtaggggccg tggctccgtc
     4921 ccggcccgcg ccgtgaggcg cccggtcaac gacaaggggc ttccccccac gtgtccgagg
     4981 cgctagggtg cctcggcgca cggatcaaga aggtgcctca cggcgcgggc cgggacggag
     5041 ccacggcctc taccgaggac ggtgaacact ctacgctctc cgcgcgcagc agttcctcga
     5101 tcgtcgagag cggaaccggc cgccagggtg atcctgtcgt gtgcttgggg ctggtgttgc
     5161 ctgggtggac cctgccgatg cagatggtcg tgtcggccag cgggacgatc ttctttggca
     5221 cgcgggagcg cagaaagcgc gtgtcttcgc cgacctgcgc gttgatgaac gggttgcgta
     5281 accagagcgc gcgggtgtag cagagcgtgt tgccggccac ccagcggccc cgccgccccg
     5341 gatagacata ctcccaggcc agtcccgccg cgggatcgtg atacagcagc cggtccagcc
     5401 cgcagacatc cgcctgcgat tcccgtagcg cgccgatctg gacccggatg cgacgcgcgg
     5461 ccatccagtc gtcgtcatcc cagtgcagca ggtagtcgcc gcctgcctcg cggcaggcga
     5521 tgttgcgctt ctcacccagc gtccgcttgc cgctgagtcg caggtagcgg atgcgcggat
     5581 cgccgtccag caggtcagcg accgcatcct cgccgtcgtc aacgacgatc agctcgcggt
     5641 tcgggtagtc ctggcgctga aagtcggcga tcgagcgcct gacgaactcg cgccgatcgg
     5701 cggtcggcat gatgcagctg acgagcggtg tcgtgtccgg cgtgacgacg atgatgggac
     5761 cggcaggtac gggcgcggac atggccggcg agagtgacgt cagaaatgcg cggtcggctc
     5821 gcaggtagtc aggctcttcg gcaggcagcc agccggccgg gtcaaggtag ctgccacacg
     5881 cgaacgacca gctgttgctc gcatggcgca ggtagacgaa ctggccgttg ttctccagca
     5941 cggccagccg cgcccgccgc tgccggcagc gccgcaggaa gatggcgtcc tcggccagcg
     6001 agcggttcgg gtacttggcg acctgctccc agaggcggcg ctgatagacg agcgtgccac
     6061 cgtggacatc gccgacgaac agccgccggt gcagctccgg cgtcgtcgtc cagaacgccc
     6121 agcggggcac gtcgaggatg acgccgccac gcagcccgct gatctcggcc tcgccggcca
     6181 gcagcggcgc aagctggcgc cgcagccggt ccggcgcgta ccagtcgtcg tcgtcccagt
     6241 gggcgatgaa cgtcccacgt gccagatcgc aggcccggtt gcgcttcgcg ccgatcgtca
     6301 gcccgcgcgg cacccgcagg tagcggatgc gtggatcggc gggtagctgc tgttccagat
     6361 cgtcgtcgcc gtcgtcaacg acgatcagtt cgcggtcggc gtagtcctgc cgctggaaga
     6421 gcgcgatcgc ctggagcgca aaccgggcac ggttgccggt cggcatgatg caactgacga
     6481 gatgtgtcgc cggctcaggc ggagcgacaa ccggcggcgg gctggccggt gcaacaggcg
     6541 gctcaggcag cgcagagggc cgcaacggga tcgcacgccg gatcaggtct ggcggcgcaa
     6601 aatgctcgca cgcctcgccc cggcaatgtg gctggtagcc gctcgctgaa ccggtcagcc
     6661 gcaagccgca gaacgcgtcc tgatagcgac ggcggctggc gtcgagcaac agacgctcgg
     6721 cccgtcgccg gcgcggcgtg aacggcgccc gctgcacgta ggcgccgcca acccaggcaa
     6781 cccggaagcc ggcccggaca gcgcggatgt tgtagtccat ctcccagcag gggccgaggc
     6841 cgtagccctc gtcggccgcg ccgatccgct cgacgacctc gcgccggacg acgtagcaga
     6901 agtcggccag gctgtgcagc ggctccaacg ttctgctgcc ggcgccgaag cgtttccgga
     6961 cctcgcccac gatcgccgcc acctcgacca gcgtgctccc cgactggcga acgacggcct
     7021 gctcgttcca ggcacggttt gtcgatggcc cgacaatacc cgtcgttgga tcggcctcga
     7081 acgcggccag cagcaactcc agccagcccg gtccaacgac cgccccgctc tccagcagca
     7141 ccagcacatc ggcgtcaccg gccagcgcca gccggttgaa acaggccggc gggccgagcg
     7201 cggcctgtgt cccaagctgg tggaggtccg gcagcgttgc gaggtagtcc acgacatccg
     7261 gctcgggacc atccggcagc aggacgacct catgcggcgg accggtgttg gcccggacgc
     7321 tggccagcgt ctccgccagt cgcctcgctt cagcgtggac gtggatgccg atcgtgacag
     7381 tcgtcatgcg aaccgctcgc agttggaacg ggagcggcgc cggttctcga tgacggccag
     7441 gccgagcctg tcgccttccg gctcgccaca ctcggccgcc cagagccggg cgacgccgta
     7501 accggccgct tcgatccgtg cgaccgcgcg gtccacgctg ccggtggcga gacagacatc
     7561 gtgaatcagg atcagcccgc ccggacagac cagcttcgtc gccagctcga actcgttcca
     7621 gacgtgttcc gcttcgtgca gcgaatcgag cagggccgcg tcgtagcgtt cgccgtcgtc
     7681 cagcgcggca gccatccccg ccaccgaatc gaggggccgc tgctccaggc aggcggacat
     7741 cgccggtggc agcatgtccc acagctcgca gcggccgggc tggtcgaacg gatcgaagct
     7801 gacgacccgc ccaccggccc gatgggccac cgccgacgcc aggcaggcgg ccgagatgcc
     7861 gcgcgccgtc ccggtctcga ggacgcggac gcagccgttc gagcgcacca ggtaatgcag
     7921 cagcgccagc agcggaaagg tcgccggatc gtgcaccgac gccgaggcgg cgtttgcagt
     7981 gccgtagaac gaccagcgca gcgcctcctg cccctgctcg ccgagccagg cgcgcaaggc
     8041 gtcaagaaac tgcccgtagc cgtcacccga tcccgctcca gccagcggct gggcgacacc
     8101 gccgtagagg ccgcgccact cgggatactt gtgccgcccg aggccgttga agtggagcac
     8161 gttcgcgtcg tgcccatgcc agcgggcagt caggcgtccg gcgctgcgag tgagatcggc
//
LOCUS       DASUGJ010000683         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_110442, whole genome shotgun sequence.
ACCESSION   DASUGJ010000683 DASUGJ010000000
VERSION     DASUGJ010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110442"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1640)
                     /locus_tag="VFV93_15535"
     CDS             complement(246..1640)
                     /locus_tag="VFV93_15535"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5166817.1"
                     /translation="MLVMKFGGTSVGSATAIANTADILAQYAANDRVVAIVSAMSGIT
                     NTLLAKAAAAAAGDSEGVDAARVALLAPHRAVLLDLLPSGDRRRETEAELVALADEAS
                     RLLYSVYVLRELSPRASDRIVSFGERMSSVLVAAVLNERGTPALAVAADRLIVTDGVF
                     GSASPLLDRTRQKAGETLAPVFAAGLLPVVTGFFGADERGITTTLGRGGSDYSAAILG
                     NALDADEIWIWTDVDGVMTADPRIIPEAHTLAKITYAEATELAYFGAKVIHPKTMHPA
                     VERDIPIWIKNSFNPAAPGTMIGPEAANGSLAKAITSISGLAAITVEGRGQIGVTDVT
                     ARTFTALARISANVYMISQASSQHSVTFVVSGDQIDQTLAALETEFEIDLSRGRVLSI
                     ASVRDLAIVAAVGGGMRGTPGVAGRVFTAMGNEQINIVAIAQGSSELNITFVIAAEEV
                     GRAVSVLHQVFMLE"
BASE COUNT          384 a          760 c          778 g          371 t
ORIGIN      
        1 gctcaacatc accttcgtga tcgccgccga ggaagtcggc cgggccgtca gcgtcctcca
       61 tcaggtgttc atgctggagg gcgagcgaca ctgtcgctgc accccaatca atccccaatc
      121 cggtaggggc gatatcgcaa tatcgccctt tggtcccacc cagacacatc gggcggggag
      181 cagggcgata tcgcaatatc gcccctacgc gattggacga ttggggtgca gcgacagagt
      241 cgcgatcact ccagcatgaa cacctgatgg aggacgctga cggcccggcc gacttcttcg
      301 gcggcgatca cgaacgtgat gttgagctcg ctggagccct gggcgatcgc gacgatgttg
      361 atctgctcgt tgcccattgc cgtgaaaacg cggcccgcga cgcccggcgt gccgcgcatg
      421 ccgccgccga cggccgcgac gatcgccaga tcgcgcaccg aggcgatcga gagcacccgg
      481 ccacggctga ggtcgatctc gaactcggtc tccagcgccg ccagcgtctg gtcgatctgg
      541 tcgccgctga cgacgaacgt caccgagtgc tgcgacgacg cctgcgagat catgtagacg
      601 ttggcggaga tgcgcgccag cgccgtgaag gtgcgggcgg tcacgtcggt gacgccgatc
      661 tggccgcgcc cttcgacggt gatggcggcc aggccggaga ttgaggtgat cgccttcgcc
      721 agcgagccgt tggcggcctc ggggccgatc atcgtgccgg gcgcagcagg gttgaagctg
      781 ttcttgatcc agatcgggat atcgcgctcg acggccgggt gcatcgtctt cgggtggatc
      841 accttcgcgc cgaagtaggc cagctcggtc gcctcggcat aggtgatctt cgccagcgtg
      901 tgtgcctcgg ggatgatgcg cggatcggct gtcatgacgc cgtcgacgtc ggtccagatc
      961 cagatctcgt cggcgtcgag cgcgttgccg aggatggcgg ctgagtaatc cgacccgccc
     1021 cggccgagcg tcgtcgtgat gccccgctcg tcggcgccga agaagccggt gacgaccggc
     1081 aacagcccgg ctgcgaagac cggcgccagc gtctcgcccg ccttctggcg ggtgcggtcc
     1141 agcagcggcg aggcactgcc gaagacaccg tcggtcacga tcagccggtc ggcggcgacg
     1201 gcaagggccg gtgtgccgcg ctcgttgagg acggccgcta cgaggacgct gctcatccgc
     1261 tcgccgaacg agacgatccg gtcggacgcc ctcggcgaca gctcgcgcag gacgtagacg
     1321 ctgtagagca gccgcgaggc ctcatcggcc agcgcgacca gttccgcctc ggtctcgcgg
     1381 cgccggtcgc cggatggcag cagatccagc aggacggccc ggtgcggcgc cagcagcgcg
     1441 accctggcgg catcaacgcc ctcgctatct ccggcagcgg cagctgctgc cttcgccagc
     1501 agcgtattgg tgatgccgct catggccgag acgatcgcga cgacgcggtc gttggcggcg
     1561 tactgcgcca gaatgtcggc cgtattggcg atcgccgtcg cgctgccgac cgaggttccc
     1621 ccaaacttca tgaccagcat ggactactgc tccccagcgt gatggattgc gaatatcaac
     1681 actgccgttt accacgccgg cagggtgttg cgctacccac gcccgccgca acaccgctcg
     1741 acgactcgaa cgttcgaccc tatctcactc tgtgacactt ttgtgatttc tgccgtccgg
     1801 ttacgtttcg ggctgccgcg cggcgatcca gtcgtcgcaa cgccgcgtac ggacgctgtt
     1861 ctgccgctcc acacgcgtga cagccagtcc taggtcatcg ccggatattg cgaatgatgc
     1921 ccgaacgtgc gcaaaactgg cctcggcagg gcttgacgga cgacagcggc tgggggtacg
     1981 aatagaagcg gtgacacaac gcatcggcgc ggcactggtg atgccgccgg ggctggagag
     2041 cgacgggcgt cgcgcagccg agccgggtcg ttgtgagtcg cggtcgtcca gccgggttgg
     2101 ctgtggctgc cgcgcggggg tgaacagcgc tggagggggg attcgaggga tcagtccggg
     2161 tgacaaaccg acagttggcg gcgtagcacg cgccccggca gtcaaccgca agcggcgttc
     2221 aaaccgccgg cgatcctcgg acgacgccaa agcaccacca gcacctccaa ccagagcgac
     2281 ctcgtcagac gcc
//
LOCUS       DASUGJ010000684         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1779628, whole genome shotgun sequence.
ACCESSION   DASUGJ010000684 DASUGJ010000000
VERSION     DASUGJ010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779628"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1720)
                     /locus_tag="VFV93_15540"
     CDS             complement(95..1720)
                     /locus_tag="VFV93_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5166818.1"
                     /translation="MSANPLTDHDQFILRSPYPDVTYPDSALTPFILRNAAQLGDKPA
                     IIDGPTGRVMTYSQLAENIRARAAGLAGAGLAKGDVLTIYAPNDPEYAVAFHAIISIG
                     AIVSTANPLLTAGELGAQLIDANARYLLTVPGLLDAARSAAATAGIPDDRIFVFGEAD
                     GVRSLASLSIPGTRPPQVAIDPEDIAVLPYSSGTTGLPKGVMLTHRNLVAQDAQLASE
                     GEVIQHDDVVIAVLPFFHIYGMSVIMNHGISQGATLVTMPRFDLPQFLQLIQDHRVTR
                     AFLVPPIVLALAKHPIVENYDLSSLQVIFCGAAPLGADLSQAAASRLDCIIKQGYGLT
                     ETSPVTHLTPDAQVKDGKIGLLVASTEALIVDIETLLPLGRDQEGEVWVRGPQIMRGY
                     LNRPEATAVTIVDGGWLRTGDIGSVDADGYFTIVDRLKELIKYKGYQVAPAELEAVLL
                     SHPSVADAAVIGSPDEECGEVPKAFVVCKEEINATDLLEYVAAHVAPYKKIRLLDFVE
                     SVPKSASGKILRRMLIEQERERVRAAAGAASPV"
     gene            1891..2298
                     /locus_tag="VFV93_15545"
     CDS             1891..2298
                     /locus_tag="VFV93_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166819.1"
                     /translation="MDEQLDGSGVEQDFYHFRDLVEDVLSPGAVGTLEQLTPRILEIY
                     GLAELLDRQLPADMREEHAERLVTRVKRVVRLLPTDVSPMPNEVFTAIEFLLYEIDGE
                     PVKIGEAILRLEILADEIRARPLLHNLWMGRAN"
BASE COUNT          424 a          693 c          918 g          426 t
ORIGIN      
        1 gatccacggg cgcaggcgtt ggggattctc gattcacaca cgcgcatcaa gcgggcgccg
       61 aggcccagag ggcaccccgc gcgggccggg acccctacac gggtgaggcg gcacctgcgg
      121 cggcgcgaac gcgctcgcgc tcctgttcga tgagcatccg gcggaggatc ttgccggagg
      181 cagatttggg gacggactcg acgaagtcga gcaggcggat cttcttgtag ggtgcgacgt
      241 gggcggcgac gtactccagc aggtcggtcg cgttgatctc ctccttgcag acgacaaatg
      301 ccttggggac ttcaccacac tcctcgtccg ggctgccgat gacggcggcg tcggcgacgg
      361 acgggtgcga gagcaggacg gcctccagtt cggccggggc aacctggtag cccttgtact
      421 tgatcagctc cttgaggcga tcgacgatcg tgaagtagcc gtccgcatcg acggagccga
      481 tgtcgccggt ccgcagccag ccgccgtcga cgatcgtgac ggccgttgcc tcggggcggt
      541 tgaggtagcc gcgcatgatc tgcgggccac gcacccagac ttcgccctcc tggtcgcggc
      601 cgagcggcag caatgtctcg atatcgacga tcagcgcctc ggtgctggcg accagcaggc
      661 cgatcttgcc gtccttgact tgcgcgtcgg gggtgagatg cgtgacaggg ctggtctcgg
      721 tcagcccgta gccctgcttg atgatgcagt cgagccggga ggctgctgcc tgcgagaggt
      781 cggccccgag cggcgcggcg ccgcagaaga tgacctgcag gctgctgagg tcgtagttct
      841 cgacaatcgg atgcttggcg agtgccagca cgatcggcgg gacgaggaac gcgcgcgtga
      901 cgcgatggtc ctggatgagt tgcaggaact gaggcaggtc gaagcgcggc atcgtgacga
      961 gcgtcgcgcc ctgggagatg ccgtggttca tgatgacgct catgccgtag atgtggaaga
     1021 agggcaggac ggcgatcacg acgtcgtcgt gctggatgac ctcgccctcg gaagcgagct
     1081 gggcatcctg ggcgacgagg ttgcgatggg tcagcatgac gcccttcggc aggccggtcg
     1141 tcccgctgga gtacggcagg acggcgatgt cttcgggatc gatcgcgacc tgcggcggcc
     1201 tggtgccggg tatcgagagc gaggcgagcg acctaacacc gtccgcctcg ccgaagacga
     1261 agatgcggtc gtcggggata ccagccgtgg cggcggcgct gcgggcagcg tcgagcaggc
     1321 cggggacggt cagcaggtag cgcgcgttgg cgtcgatcag ttgcgcgcca agctcgccgg
     1381 ctgtgaggag cgggttggca gtcgagacga tggcgccgat cgagatgatg gcgtggaagg
     1441 cgacggcgta ctccggatcg ttcggcgcat agatcgtcag aacatcgcct ttggctagcc
     1501 cggcgcccgc caggccggcc gcgcgggcgc ggatgttctc agcgagctgg ctgtacgtca
     1561 tgacacggcc ggtcgggccg tcgatgatgg ccggcttgtc gcccagctgg gcggcgttgc
     1621 gcaggatgaa cggcgtcagt gcgctgtcgg ggtaggttac atccggatac gggctgcgaa
     1681 gaatgaactg gtcatggtcg gtcaacgggt tggcggacat gaatggttcc tttcctgcgt
     1741 aggggtgccg tggcgacaga gaacgatcgc aagagattgc tggcaaacgg tggcgggatc
     1801 gtaggagcag tgcaacgacg ctgtcaagac catcaggccg ggccggtatg attcggtgga
     1861 atgggataga cagggaacgc aggaggcacg atggacgagc agcttgatgg ttcaggtgtc
     1921 gagcaggatt tctatcactt tcgcgacctt gtcgaagatg tcctctcgcc cggagcggtc
     1981 ggcacgctgg agcaattgac gccgcgcatt ctggaaatct acggactcgc agagttgctg
     2041 gaccggcagt tgccggccga tatgcgcgaa gaacatgccg agcggctggt gacacgagtc
     2101 aagcgggtgg tacggctcct gccgactgat gtctcgccga tgccaaacga ggtctttacg
     2161 gcgattgagt tcctgctcta cgagatcgac ggcgaaccgg tcaagatcgg cgaggcgatc
     2221 ctgcggctgg agatcctggc cgacgagatt cgcgcacgac cgctattgca caatctgtgg
     2281 atggggcggg cgaattagcg cgatgcgtca cgcatcgtcc acaaacgaca acgtcatcgc
     2341 gaggccgggg gtgccggcgg tggcggttgg gaactcctgc tgggcgacgg tggtgatcgc
     2401 gttcaggtct tcgacgctgg ggctgaagtg ggtcagccag aggtggcgga cgttggcccg
     2461 g
//
LOCUS       DASUGJ010000685         4566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_666964, whole genome shotgun sequence.
ACCESSION   DASUGJ010000685 DASUGJ010000000
VERSION     DASUGJ010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666964"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFV93_15550"
     CDS             complement(<1..594)
                     /locus_tag="VFV93_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166820.1"
                     /translation="MSVATRSETASTTLHDADSHDVIRVHGARENNLKDVSIAIPKRR
                     LTVFTGVSGSGKSSLVFGTIAAESQRLINETYSAFVQGFMPTLARPEVDVLDGLTTAI
                     IVDQERLGANPRSTVGTVTDANAMLRILFSRLGQPQIGPPNAFSFNVASVTASGAITV
                     DRGGKTKTEKATFSRLGGMCPRCEGRGSVTDFDLSQLY"
     gene            complement(636..1034)
                     /locus_tag="VFV93_15555"
     CDS             complement(636..1034)
                     /locus_tag="VFV93_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166821.1"
                     /translation="MSALDRLLGTWDISMHHSAMSEPVSGRQRYERVLDGAFVLLTWT
                     YDHPDFPDAMALLSDDRCHYFDVRGVVRVFDFAIDEAGWSMIRLDDEFSQRYTTQFRG
                     PDVMESAGEASFDRGASWRPDFTMTYQRVP"
     gene            complement(1151..1582)
                     /locus_tag="VFV93_15560"
     CDS             complement(1151..1582)
                     /locus_tag="VFV93_15560"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5166822.1"
                     /translation="MADSTTANDLTIQTTFLPLDDPDASLAFYRDILGFEVRADVGQG
                     AMRWITVGPADQPGVSIVLNPPFADPSISDEERSAVSAMMARGSYAMIILATKDLDGT
                     FERLKANGAEVIQEPTEQPWGARDCAFRDPAGNVVRINEQR"
     gene            complement(1723..2721)
                     /locus_tag="VFV93_15565"
     CDS             complement(1723..2721)
                     /locus_tag="VFV93_15565"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5166823.1"
                     /translation="MEYRGLGATGLNVSRLGIGLAALGRPGYITLGHASDFAGGREVE
                     AMSWQTHKVLDAAWELGIRYVDAARSYGRAEEFLAGWISSRGVGSAPLVVASKWGYTY
                     AAGWRTTAEQHEVKDHSLATLNRQYGESRAILGAWLRLYQIHSATLASGVLNKPDVLD
                     RLAALKRQTGLLIGLTLSGPESWSVLVRATGVERDGRPLFDVVQATWNLLEPSLAALL
                     FQAHQSGMGVVVKEALANGRLTDRNDDPAFAGKRFILEREASRLGCTIDQLALAAVLE
                     RPWADIVLSGASTPVQLRSNVAALNVRFDAQAREALSWLAEPVEQYWETRSRLPWN"
     gene            complement(2823..4271)
                     /locus_tag="VFV93_15570"
     CDS             complement(2823..4271)
                     /locus_tag="VFV93_15570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5166824.1"
                     /translation="MQRPNPAPHHSSKHFRTLLVEPDAQTRQDIQFELSVRGQAVVDC
                     PDTAAAMALFHDNVFALVLIGSRLPDDDGLALCRDIRALPGGDETVIIVVSQAPSDND
                     LAAAIEAGADDCISWTADGDQLADQIETIMTAARERMLPDGAAGRGACERFLVINTNG
                     TIREAAPVAERLLGFPPEAIVGVNAFSFFHPDDAPQLLSMITESFTGTGRTRAIEARV
                     RRDGDSWQTVTISATNQTTDPRVQGVAFELRGPDANVGSDDQVTRAAMHDRLTDLPNR
                     NLFIDRIDHAVVRASRSQQPIVVMVIDFADYVAADGQTQADDDDGLVIAVAQRLRSCL
                     RSSDTAARLGHDQFGILLEEILAVDNIEIVSDRIIRAMSVPFYTGGTELTLTAHIGVA
                     VNTPNRNRAVDLLRDANIARAWARVQGSGSHVLFDPSMLPPDDESTTSEFAVGVATPV
                     APAPGLDQRLDELNQRIASLEQTLARFAPVVE"
BASE COUNT          762 a         1458 c         1516 g          830 t
ORIGIN      
        1 gtacagctgc gacagatcga aatcggtcac cgagccgcgg ccctcgcagc gcgggcacat
       61 gccgccgagg cgggagaatg tggccttttc ggtcttggtc ttgccgccgc gatcgaccgt
      121 gatcgccccg ctggctgtga cggaggcgac gttgaaggag aatgcgttgg gcgggccgat
      181 ctgcggctgc ccgagccggc tgaagaggat cctcagcatc gcgttggcgt cggtgacggt
      241 gccgactgtc gagcgcgggt tggcgcccag ccgctcctga tcgacgatga tcgccgtcgt
      301 cagcccatcg aggacatcga cctccggacg cgccagcgtc ggcatgaagc cctggacgaa
      361 agcgctgtag gtctcgttga tcagccgctg agactcagcc gcgatcgtcc cgaacaccag
      421 cgagctcttg cccgagccgg agaccccggt gaagaccgtc aaccggcgct tcgggatcgc
      481 gatgctgacg tccttgaggt tgttctcgcg cgcaccatgg acgcggatca catcatggct
      541 gtcggcatcg tgcagcgtag ttgacgccgt ctctgatctc gtggctacgc tcatcatctc
      601 tccatctgtt catcggccag gtggtcttcg tcggtttacg gcacgcgttg gtacgtcatc
      661 gtgaagtcgg gccgccagct ggcgcctcgg tcgaacgatg cctcgccagc gctctccatc
      721 acatccggtc cacggaattg tgtcgtgtag cgttgggaga actcatcgtc caggcggatc
      781 attgaccagc cagcctcgtc gatcgcgaag tcgaaaacgc ggacgacccc acggacgtca
      841 aagtaatggc agcggtcatc cgacagcagc gccatcgcgt ccgggaagtc gggatggtcg
      901 tacgtccagg tcagcaggac gaacgcaccg tccagcaccc gctcgtagcg ctggcggcca
      961 ctgaccggtt cggacatggc tgagtgatgc atgctgatat cccacgttcc gagcagccga
     1021 tcgagtgcgc tcatctctgc tccattcgtt tgattgccgg tcaacccgtt gccaggtcag
     1081 ccggcgcgga cgagttccaa cgaatgatag aacatgtccg tacaggtcat cccgacgctg
     1141 gccggtcgga tcagcgctgc tcgttgatgc ggacgacgtt gccggccgga tcgcggaagg
     1201 cgcagtcgcg agcgccccac ggctgctcgg ttggctcctg gatgacctca gcaccgttcg
     1261 ccttcagccg ctcgaaggtg ccgtcgaggt ccttcgtcgc caggatgatc atcgcgtacg
     1321 agcctctggc catcatcgcg ctgacggcgc tgcgctcctc gtcgctgatg ctggggtccg
     1381 cgaacggcgg gttcaggacg atcgagacgc cgggctgatc ggccgggcca actgtgatcc
     1441 agcgcatggc gccctgcccg acatcggcgc gcacctcgaa gccgaggatg tcgcgataga
     1501 aggccagcga ggcgtccgga tcgtcgagcg gaaggaaggt cgtctgaatc gtgaggtcgt
     1561 tggctgtggt cgagtctgcc atctagcgct cctgatactg tctacgctcg tgacgcgcga
     1621 actgcccgtc atctctctga cggatcgcac ggcagaaacg tgacaaatgc cgttggaaac
     1681 gcacccgcgc gagatcgcgg tgcgtgttcc tcgcggccga actcagttcc agggcagcct
     1741 tgagcgcgtc tcccagtatt gctcgaccgg ttcggcgagc cagctgagag cctcgcgggc
     1801 ctgtgcatcg aagcgaacgt tgagggcggc gacgttggag cgcaactgca ccggtgtcga
     1861 ggcgccggag aggacgatgt cggcccacgg gcgttcgagg acggcggcca gcgcgagctg
     1921 atcgatcgtg cagccgagcc gagatgcctc acgctccagg atgaagcgct tcccggcgaa
     1981 ggcggggtcg tcgttgcggt cggtcaaccg gccgttcgcg agcgcctctt tgacgacgac
     2041 gcccatcccg gattggtgcg cttgaaacag caacgcggcc agcgacggct ccagcaggtt
     2101 ccaggtcgcc tggacgacat cgaacagcgg ccggccatcg cgctcgacgc cggttgcccg
     2161 caccaggacg ctccacgact caggtccgct cagcgtcagc ccgatcagga ggcccgtctg
     2221 gcgcttcaac gccgcgagtc ggtccagcac atcgggcttg ttgaggacgc cggaggccag
     2281 tgttgccgaa tggatctgat agaggcgcag ccacgctccg aggatcgccc gcgattcgcc
     2341 gtactgacgg ttgagtgtcg ccagcgagtg gtccttgact tcgtgttgtt cggcggtcgt
     2401 ccgccagccg gcggcgtagg tgtagcccca cttgctcgcg acgaccagcg gggccgaacc
     2461 gacgccgcga cttgagatcc aaccggccag gaactcctcg gcccggccat aggaacgggc
     2521 cgcatccacg tagcggatgc cgagctccca ggccgcgtcg aggaccttgt gggtctgcca
     2581 gctcattgcc tcgacctcgc gcccaccggc gaagtcagaa gcatggccga gcgtgatgta
     2641 gccgggccgg ccgagtgccg ccagcccgat tcccaaccgt gagacgttca gcccggttgc
     2701 gccaagcccg cgatactcca tgccccatcc tcccacggaa tcagggactt attatccgcg
     2761 aaccggcggt cttctgcctg ccgattcgcg gatgattgga tgggattgga gcaggagcgc
     2821 tgttattcga cgactggtgc gaagcgcgcc agcgtttgtt cgagcgacgc aatccgctgg
     2881 ttgagctcat cgagccgttg gtcgaggcct ggtgctggcg ccaccggcgt ggctacaccg
     2941 acggcgaact cgctggtggt tgactcgtcg tcgggcggca gcatactcgg atcgaacagg
     3001 acatggctgc cggaaccctg gacacgggcc caggccctgg cgatgttcgc gtcgcgcagc
     3061 aggtcgactg cacggttccg attcggcgtg ttgacggcga cgccgatgtg cgccgtcagc
     3121 gtcagttccg tgccaccggt gtagaacggc accgacatcg cgcggatgat ccggtcggac
     3181 acgatctcga tgttgtcgac cgccaggatc tcttccagca ggataccgaa ttggtcgtgg
     3241 ccgagccggg cggccgtgtc gctggagcgc aggcacgaac gcagccgctg ggcaacggcg
     3301 attacgagac cgtcgtcatc gtccgcctgg gtctgtccgt cggccgccac gtaatcggcg
     3361 aagtcgatca ccatcacgac gatcggctgc tggctgcgcg acgcgcgaac gactgcgtga
     3421 tcgatccggt cgatgaacag gttgcggttg ggaagatcgg tgagtcggtc gtgcatggcc
     3481 gcccgggtga cctgatcatc gctgccgaca ttcgcgtccg gaccgcgcaa ctcgaaggct
     3541 actccctgga cgcgtggatc ggtcgtctgg tttgtggccg agattgtcac ggtttgccac
     3601 gagtcgccgt cacggcgcac gcgggcttcg atggcgcgcg tccggcccgt accggtgaac
     3661 gactcggtaa tcatgctcaa caactgcggc gcgtcatccg ggtggaagaa gctgaacgcg
     3721 ttgacgccaa cgatcgcctc gggcgggaac ccgagcaggc gctcggcgac cggcgcggcc
     3781 tcgcggatcg tgccgttcgt gttgatgacc aggaaccgct cgcaggcgcc tcggccggct
     3841 gccccgtccg gcaacattcg ttcgcgggcg gccgtcatga tcgtctcaat ctggtccgca
     3901 agctggtcgc cgtcggccgt ccagctgatg cagtcgtcgg cgccggcttc gatcgcggcc
     3961 gcgaggtcgt tgtccgaggg cgcctggctg acgacgatga tgaccgtctc gtcgccgccc
     4021 ggcaaggccc gaatgtcgcg gcagagcgcc agtccatcgt cgtccggcag ccggctgccg
     4081 atcaagacca acgcgaagac attgtcatga aagagcgcca tggccgcggc ggtgtcggga
     4141 cagtcaacaa cggcctggcc ccgcactgag agctcgaact ggatgtcctg gcgtgtctgg
     4201 gcatccggct caaccagcag cgtcctgaaa tgcttcgaac tgtgatgtgg cgccgggttg
     4261 ggtcgttgca tcaggcagcc cttcgttggt gtcgtcgttc catgtgacat tgcatcacgg
     4321 ggcggccggg tccgctcgcc aatccggccc gcgctgccgt ctctatgacg taacgatacg
     4381 gggattttgt tgatactgga aaggggaaga acgtcccgct tcaacgggta ctcaacgtac
     4441 ctgatttcgg cacatcctct gtcgtcctga atagtcaacc acggtgttcg tgacgggttg
     4501 acggcgatgc gaggatgcac gagttcccct gtcgtcctga gccgcagcga aggacctctc
     4561 cgcctt
//
LOCUS       DASUGJ010000686         5296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1361985, whole genome shotgun sequence.
ACCESSION   DASUGJ010000686 DASUGJ010000000
VERSION     DASUGJ010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361985"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFV93_15575"
     CDS             complement(<1..235)
                     /locus_tag="VFV93_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166825.1"
                     /translation="MIDQFVHNRRPRWQQLEGLLARGRGRQGARLSATELEDLGQLYR
                     QASSDLAIVRRDFPRDRVTRYLESLVSRAHPIVY"
     gene            283..1137
                     /locus_tag="VFV93_15580"
     CDS             283..1137
                     /locus_tag="VFV93_15580"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5166826.1"
                     /translation="MDPEDTRYRVETPEQIGLEYDIAGLGTRLMAAIVDVIFLGLIAG
                     FALCLGVFGMTAVFATFSDSNTASAIAIAIVGLIFFFVVWGYYVLFETIWHGQSPGKR
                     WTGLRVIQEGGYPIGFSQAAIRNIVRLADFLPFLYIIGAIVMLVDSRSRRLGDLVAGT
                     IVVKEQQEVSLATLGHDQPPPAPWPASAMGQSFPGLQTQAVHASQFPNLARISSADYS
                     LLREFLQRRATLDPAARGALALQLAQGFARRLDYTPAGDVPEQFLQRLAADLANRQVV
                     PQSQGPAF"
     gene            complement(1256..2461)
                     /locus_tag="VFV93_15585"
     CDS             complement(1256..2461)
                     /locus_tag="VFV93_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEX5166827.1"
                     /translation="MAYETVINGGRVIDPATGVDGTLDVAIADGKIAAVAANIDAAGA
                     KVIDASGQIVTPGLIDLHTHIYWGVTYWGIEPDPVAAKTGVTTWLDVGSSGGYTFPGF
                     RRYVAEPSKVRVFALLNLSVIGLVAPTWELSNLDYCNVDLGAMMIDANRDLILGIKAR
                     IDRNTTRGTGIRPLELARELADRVGLPLMVHIGAGPPTIDEVAHLLRPGDILTHCFTG
                     GDMKIMTDDGKINPAIKDLQERGLVLDIGHGTGSFSFQTTDAMLAQGVLPDVISSDIH
                     QMARQGPMFDMPTTLSKFLNLGLSLPDVIDRATARPAAAMRRPDLGTLATGSLADVAI
                     WRIDEGEFTFYDVDLNPRSGTMQIVNTLTMVGGEVLDEQPELPMHPWTKLPDKQQPIL
                     GHQPGERVK"
     gene            complement(2580..3422)
                     /locus_tag="VFV93_15590"
     CDS             complement(2580..3422)
                     /locus_tag="VFV93_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5166828.1"
                     /translation="MAINDPCIISVAITGSGTTLAQNPNLPCTPEQIANSAIESWSAG
                     AAIAHIHVREADGTPTHNMDYYREIIERVTAESDIVLNLTTGGFTGLEPHKRLETLTL
                     EPELGSFDAGSMNFGEGVFLNPPSFLREMAASFQEYNVRPELECFDVGMIYNCLGLIQ
                     EGAIDGPLWWQFVLGVKGGAPATPKVLLHMVELLPPGSQWSAIGLGRGQLPMNTMAII
                     MGGHVRTGMEDNVYYSYGELATGNAQLVERCARLTRELGREVATPADARRLLELKGGI
                     GVRG"
     gene            complement(3526..>5296)
                     /locus_tag="VFV93_15595"
     CDS             complement(3526..>5296)
                     /locus_tag="VFV93_15595"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HEX5166829.1"
                     /translation="GKQAVVVGDDKQLPPTSFFDTMASADEIDETTVSDIPSILGLFN
                     SQSAPKRMLRWHYRSRHESLIAVSNHEFYENKLVIFPSPDHARTEAGLVFHHLPDTSY
                     DRGGSRTNRDEARIVAEAVMQHARRSPELTLGVAAFSNPQAEAILAEVERLRREDPTC
                     EAFFSAHPHEPFFVKNLENVQGDERDVIYISVGYGRTSEGYVSMNFGPLNADGGERRL
                     NVLITRSRLRCEVFTNLTADDIDLRRSQARGVAAFKHFLKYAQTGIMDVPEETGRASG
                     SPFEDAVAEALRGSGYTVEGQVGSAGFFIDLAVVDPRRPGRYLLGIECDGATYHSARS
                     ARDRDRLRQQVLEGLGWTIHRIWSTDWFRNAERELTRLIAAIEAAAAQGETTEIPIPT
                     TTETTIERAETDDAPPDSGIPAYVLAEPNVRTNGLELHAVPAERLADWLVKVVTVESP
                     IHSDDAIRRIADAAGVSRIGSRIRAVLEAAIVKASLDGRIEQRGPFLWSAGMVLAPVR
                     DRSNISNRSLTSIAPEEIAAAIRLAIANAYGMEQSQVAAAACRLLGFPRLTDEMRGTV
                     DPLVEALLASGQLQRQGNHLTVT"
BASE COUNT          811 a         1805 c         1737 g          943 t
ORIGIN      
        1 ggtagacgat cggatgggcg cggctgacga ggctctccag gtagcgcgtg acacggtcgc
       61 gggggaagtc gcgccggaca atcgcgaggt cggaggacgc ctgacggtac aattgcccca
      121 ggtcctcaag ctccgtcgcg ctcagccgag ctccctggcg gccacgaccg cgcgccagca
      181 acccttcgag ctgctgccag cgcgggcgac ggttgtggac gaactggtcg atcatcagga
      241 aggcacgctc tctccgtggt cagcccacag tcaccgcgac agatggatcc tgaggatact
      301 cgctaccggg tcgaaacgcc cgagcagatc ggcctagaat atgacatcgc cgggctcggc
      361 acgcggctga tggctgccat cgtcgacgtg atcttcctcg gactgatcgc cgggttcgcg
      421 ctctgcctcg gtgtcttcgg gatgacggcc gtcttcgcca ccttcagcga ctccaacacc
      481 gccagcgcga tcgcgatcgc gatcgtcggc ctgatcttct tcttcgtcgt ctggggctac
      541 tacgtcctct tcgagacgat ctggcatggt caatcgcccg gcaagcgctg gacgggacta
      601 cgcgtcattc aggagggtgg ctatccgatc ggcttctcgc aggccgcgat ccgcaacatc
      661 gtccggctgg ccgacttcct gccgttcctc tacatcatcg gcgcgatcgt gatgctggtc
      721 gattcccgct cgcgccgcct cggcgatctc gtcgccggca cgatcgtcgt caaggagcaa
      781 caggaggtct cgctcgcgac actcggccac gaccaacccc cacccgcacc gtggcccgcc
      841 tcggcgatgg gacagtcgtt tccgggcctc caaacccagg ccgtccatgc gtcgcagttc
      901 cccaacctcg cccggatcag ttctgccgac tacagcctgc tgcgtgagtt cctgcaacgc
      961 cgcgcgacac tcgaccccgc cgcccgcggt gccctcgcgc tccaactcgc tcagggcttc
     1021 gcacggcgcc tcgactacac cccggccggc gacgtccccg agcagtttct ccagcgactc
     1081 gcagccgacc tcgccaaccg ccaggtcgtg ccgcaatctc aaggaccagc gttctgagga
     1141 accgaccgca cgcaaagaag taggggccgt gcctcgggcc ggccctgctc cccacccgat
     1201 gcattcgggc aaccgagcag ggccggcccg agaccggccc ctacttcatt cagcgttact
     1261 tgacccgttc gcctggttgg tgaccgagga tcggctgctg cttgtcgggc agcttcgtcc
     1321 acgggtgcat cgggagttcc ggctgctcgt caagaacctc gccgccaacc atcgtcagcg
     1381 tgttgacgat ctgcatcgtg ccgctgcggg gattgaggtc gacgtcgtag aaggtgaact
     1441 cgccctcatc gatgcgccag atcgcgacat cggccaggct gccggtcgcc agcgtgccga
     1501 ggtcagggcg gcgcatcgcg gcggccggtc gggcggtcgc acggtcgatg acatctggca
     1561 ggctcaggcc gagattgagg aacttcgaga gcgtcgtcgg catgtcgaac atcggtccct
     1621 ggcgtgccat ctgatgaatg tcgctggaga tgacgtccgg caggacaccc tgagccagca
     1681 tcgcgtcggt tgtctggaac gagaacgagc cggtgccgtg gccgatgtcg aggacgagtc
     1741 cccgctcctg cagatccttg atggccgggt tgatcttgcc gtcgtcggtc atgatcttca
     1801 tgtcgccgcc ggtgaagcag tgcgtcagga tatcgccggg ccgtagcagg tgggcgacct
     1861 catcgatcgt cggcggcccg gcgccgatat ggaccatcag cggcaatcca acccgatcgg
     1921 ccagttcacg ggccagctcc agcggccgga tgccggtgcc gcgcgtcgtg ttgcggtcga
     1981 ttcgcgcctt gatacccagg atcaggtcgc ggttggcgtc gatcatcatc gcgcccagat
     2041 cgacgttgca gtagtcgagg ttggatagct cccaggtcgg ggcgaccagc ccgatcaccg
     2101 acaggttcag caacgcgaaa acacgcacct tgctcggctc ggcgacgtag cgccggaagc
     2161 ccgggaacgt gtagccgcca gagctgccca catccagcca ggtcgtcaca cccgttttgg
     2221 cagcgaccgg gtccggctcg atgccccagt aggtcacgcc ccagtagatg tgcgtgtgga
     2281 ggtcaatcag acccggtgtc acgatctgcc ccgacgcgtc gatcaccttc gcgccggctg
     2341 cgtcgatatt cgccgcgacc gccgcgatct tgccatccgc aatcgcgaca tcgagcgtcc
     2401 cgtcgacacc cgtcgccggg tcgatcacac gaccgccgtt gatcactgtc tcgtatgcca
     2461 tcacccactc ctctcacgaa ttcccctgtg ccgcagtatc gcacggttcg ggacggccgc
     2521 gctcagaacg acgtgacctg tgtaggggcc catacaggat cggcccctgc acggggaggc
     2581 tatccccgaa cgccgatgcc gcccttcaac tccagcaacc ggcgggcgtc ggccggcgtg
     2641 gcgacctcgc ggccgagctc gcgagtcagg cgggcgcagc gttcgaccag ttgcgcgttg
     2701 ccggtcgcca gctcgccgta gctgtagtaa acgttgtcct ccatgccggt ccggacgtgg
     2761 ccgcccatga tgatcgccat cgtgttcatc ggcagctggc cgcgcccgag gccgatggcc
     2821 gaccactgcg agccgggtgg cagcagctcg accatgtgca gcagcacttt cggcgtggct
     2881 ggcgcgccgc ccttgacgcc cagcacgaac tgccaccaga gcggcccgtc gatcgcaccc
     2941 tcctggatca gcccgaggca gttgtagatc atgccgacgt cgaagcactc cagctccggc
     3001 cggacgttgt actcctggaa cgaggccgcc atctcgcgca gaaagctggg cgggttgagg
     3061 aagacgccct cgccgaagtt catgctgccg gcgtcgaacg agccaagctc cggctcgagt
     3121 gtcaacgtct ccagccgctt gtgcggctcc agcccggtga agccgccggt cgtcaggttg
     3181 aggacgatgt ccgactcggc tgtcacgcgc tcgatgatct cgcggtagta gtccatgttg
     3241 tgggtcggcg tgccatccgc ctcgcggacg tggatgtggg cgatagccgc gccggcgctc
     3301 cacgactcga tcgccgaatt ggcgatctgc tccggcgtgc agggcaggtt gggattctgc
     3361 gccagcgtcg tccccgaccc ggtaatcgca acactgatga tgcacggatc attgatggcc
     3421 acggcccctc gcccttctct ctgtctgctg ctcgatgcgg gctatcttcg cacaggtcgt
     3481 ggcctcgttg gcgccgtccg tccggggtgc gtgggaactg cgcgtttacg tcaccgtcag
     3541 gtggttcccc tgccgctgga gttggccgct ggcgagcagc gcttcgacca gcgggtcgac
     3601 cgtgccgcgc atctcgtcgg tcaggcgggg gaagccgagc aggcggcagg cggcggcggc
     3661 gacctgggat tgctccatcc cgtaggcgtt ggcaatcgcc agccggatcg cggcggcgat
     3721 ctcctccggc gcgatgctgg taagcgagcg gttgctgatg ttgctgcggt cgcggaccgg
     3781 cgcgaggacc atcccggccg accagaggaa tggcccacgc tgctcgatgc ggccgtccag
     3841 cgacgccttg acgatggcgg cttccagtac cgcccggatc cggctgccga tccggctcac
     3901 gccggccgcg tccgcgatcc ggcggatggc gtcgtcgctg tggatcggac tctcgaccgt
     3961 cacgaccttg acgagccagt ccgccagccg ctcggccggg acggcatgca gctccaggcc
     4021 gttagttcga acgtttggct cggccagcac gtaggccggg ataccactat ccggcggagc
     4081 gtcatcggtt tcggcccgtt cgatcgtcgt ttcggtcgtc gtcgggatcg ggatctcggt
     4141 cgtctcgccc tgggcggctg ccgcttcgat cgcggcgatc aggcgcgtca gctcccgttc
     4201 ggcgttgcgg aaccagtcgg tgctccagat gcggtggatt gtccagccaa gtccctcaag
     4261 cacctgctgg cgcagccggt cgcggtcgcg ggccgagcgg gccgagtggt aggtcgcgcc
     4321 gtcgcactcg atgcccagca ggtagcgccc cggccggcgc ggatcgacga cggccaggtc
     4381 gatgaagaag ccggccgagc cgacctgccc ctcgacggtg tagcccgacc cacgcagcgc
     4441 ctcggcgacg gcatcctcga acggcgagcc ggatgcgcgt cctgtctcct ccggcacgtc
     4501 catgatcccg gtctgggcgt acttcaggaa gtgcttgaag gcagccacgc cgcgtgcctg
     4561 gctgcgccgc aggtcgatgt cgtcggccgt cagattcgtg aatacctcac agcgcaggcg
     4621 cgagcgggtg atcagcacgt tgagccgccg ctcgccgcca tcggcattca gcggcccgaa
     4681 gttcatcgag acgtagccct cgctcgttcg tccgtagccg accgagatgt agatcacgtc
     4741 gcgctcgtcg ccctggacgt tctccaggtt cttgacgaag aacggctcat gcgggtgggc
     4801 gctgaaaaat gcctcacagg tcgggtcctc gcggcgcaag cgctcgacct cggccaggat
     4861 cgcctcggcc tggggattcg agaaggcggc gacgccgagc gtcagctccg gcgatctccg
     4921 cgcgtgctgc atgactgcct cggcgacgat ccgcgcttcg tcgcggttgg tgcggctgcc
     4981 accccggtcg tagctggtgt ccggcaggtg gtggaagacg agcccggcct cggtccgagc
     5041 gtggtcgggg ctggggaaga tcaccagctt gttctcatag aactcgtggt tggagacggc
     5101 gatcagcgac tcgtgacggc tgcggtagtg ccagcgcagc attcgcttcg gcgcgctctg
     5161 tgagttgaac agcccgagga tgcttgggat gtcactgacg gtcgtctcat cgatctcgtc
     5221 cgcgctcgcc atcgtgtcga agaagctggt cggcggcagt tgcttgtcgt caccgacgac
     5281 gacggcctgc ttgccc
//
LOCUS       DASUGJ010000687         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_530296, whole genome shotgun sequence.
ACCESSION   DASUGJ010000687 DASUGJ010000000
VERSION     DASUGJ010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530296"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..760)
                     /locus_tag="VFV93_15600"
     CDS             complement(11..760)
                     /locus_tag="VFV93_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166830.1"
                     /translation="MSSTRDTIMIVDDHPLFRQGLRRLIEDGSRFTVVAEASTGFEAI
                     RLADIHQPAIILSDIQLPGVTGLKIARILKKQHPSTHIIIVSMHIDDDRLFDAVRAGA
                     SAFLSKDIEAQQLLDAIARVSNGEQLINQTVLQRPHLAWKVLSEFRSLADRGDGSAIP
                     KPPQSSIPLSVREIEVLDCVAQGLSNKEIADALYITEQTVKNHMTAVLRKLEVTDRVQ
                     AVLFAVKHGWVEIGSLQPGDIPDDTRNLEVE"
     gene            complement(916..>2922)
                     /locus_tag="VFV93_15605"
     CDS             complement(916..>2922)
                     /locus_tag="VFV93_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166831.1"
                     /translation="EGSDVSPSPTAAPAIADEGGNWEALPALTPAASLAIPISGPCNL
                     DALVLRAASDVPTSATIHLLDATGRLLWQETASLDGHIIQLQPPLDRATCDEHFVGAI
                     TIAADADVIAELIDSASGLRILPAQPAATNWLLGPLPRDRFATLEATARLTLSNPTSN
                     PVDASIRLLTESDTLLRATSVSLQPGATVALDVDLQNGVAVSVTASAPIVAGAVGWLP
                     GGSYAIPLMRETAEWQFGVQPASDRDAGVWLALANDSSDPRHVTLRDADTRRELTICA
                     GCAAMLHLSSDDAWRTASLTSDGGPIAVAAVAYQERTGSLHFDLGLPLLAALPVRIAG
                     PDGAPIVSSIAVLVLAVGLFELLRRIGVAEPLAARSAGAVALLAPLAPYAVRLYTEPL
                     AACLIVWSLVCWDTARRRPLFAIASLTLAGALPLVHGRYTPLAAVLAALAVSAGLSHA
                     RASRRQVLALAAVAVVVIAAVAVSPLAVALRDRAGLGYFSTEWVSRNLPGILLDRGSG
                     LLPFAPWILLSFAAPRSLKPLQWVAILLFLVQLSVVMLRAGGWQTFGAPARYMLPVVP
                     LLALLAAPGAARLWRSLAGRLTISALLIWSAAVTFLLLWVPLSGYISAGRYFIDDAVD
                     GLLPLEPLSAFPAIAPAAGSNLVGLLLLLLVWGCATSAVLRARR"
BASE COUNT          547 a          869 c         1072 g          434 t
ORIGIN      
        1 gttgacgacg ctattcgact tcgaggttgc gcgtgtcgtc gggaatatcg cccggctgta
       61 gcgagccgat ctcgacccat ccatgcttga cggcgaacag tacggcctgg acgcgatcgg
      121 tgacttcgag cttgcgcagg acggccgtca tgtggttctt gacggtctgc tccgtgatgt
      181 agagggcgtc ggcgatctcc ttgttcgaga gtccctgcgc gacgcagtcg agcacctcga
      241 tctcgcgaac cgagaggggg atcgacgatt gaggtggctt ggggatcgct gagccatcgc
      301 cgcgatcggc caggctgcgg aactccgaga gtaccttcca ggccaggtgt ggccgctgga
      361 ggacggtctg gttgatgagc tgctcgccgt tggagacacg cgctatcgcg tcgagcaact
      421 gctgggcttc gatgtccttc gacagaaacg cagacgcgcc ggcacggacg gcgtcgaaca
      481 gccggtcgtc gtcgatgtgc atcgagacaa tgatgatgtg ggtggatggg tgctgcttct
      541 tgagaatgcg ggcgatcttg aggccggtga cgcccggaag ctggatgtcc gagaggatga
      601 ttgccggctg gtggatgtcg gcgaggcgga tggcttcgaa tccggtcgag gcttcggcaa
      661 cgacggtgaa ccgtgatccg tcttcgatca gtcgccgcaa cccctggcga aagaggggat
      721 ggtcgtcgac gatcatgatc gtgtcgcggg tgctggacat cggctgcacg cacacaatct
      781 acggaccggt ctgtccgtga ggtcgtccga cacgccggag gcattgttcg attggcggtc
      841 aacggccatc gtcgcatttg aaccactaga ctaccagaaa cgaccgcgat ctggcgagag
      901 ttttgacgtc gggcgtcatc ggcgggcgcg caaaacggca ctcgtcgcgc atccccagac
      961 cagcagcaac agaagcaacc caaccagatt cgagcccgct gctggagcaa tggcaggaaa
     1021 ggcgctgagc ggctccagcg gcagcaaccc atcgacggcg tcgtcgatga agtagcgtcc
     1081 tgcagagatg tagccactca gtggcaccca gagcagcagg aacgtgacgg ccgcgctcca
     1141 gatgaggagc gcagagatgg tcaggcgacc ggcgagtgag cgccagaggc gggccgcgcc
     1201 gggagcggcc agcagggcga gcagcggcac gaccggcagc atatagcgtg ccggagcgcc
     1261 gaatgtctgc cagccacctg cccgcaacat cacgaccgag agctgaacga gaaacagcag
     1321 gatcgcgacc cattggagcg gtttcaggct ccgtggcgcc gcgaacgaca agaggatcca
     1381 gggagcgaac ggcaacagcc cgctgccacg gtcgaggagg atgcccggca ggttgcgcga
     1441 cacccactcg gtcgagaaat agccaagtcc ggcgcgatcg cgaagggcga cggcgagcgg
     1501 gctgaccgcg acggccgcga tgacgacgac ggcgacggcc gccagcgcga gtacttgtcg
     1561 ccgcgacgct cgagcatggc tgagtccggc gctgacggca agcgcggcga gcacggccgc
     1621 aagcggtgtg tagcggccgt ggacgagcgg gagggcgccg gcgagcgtca gcgaggcgat
     1681 tgcgaagagg ggtcgacgcc gggcagtgtc ccaacagacg agcgaccaga cgatcaggca
     1741 ggcggccagc ggttcggtgt agagccgaac cgcgtacggt gcgagcggcg ccaacagtgc
     1801 aacggcgccc gcgctccggg cagcaagcgg ctccgcgacg ccgatccggc gcaacagttc
     1861 aaagaggccg accgccagga caaggacggc gatggaggaa acgatcggag cgccgtccgg
     1921 accggcaatg cggaccggca acgctgccag cagcggcagg ccgaggtcga agtgcaacga
     1981 cccggtacgt tcctggtagg ccacggcggc gaccgcgatc ggtccgccgt cgcttgtcag
     2041 gctggccgtg cgccacgcat cgtcgctgga caggtgtagc atcgcggcgc agccggcgca
     2101 gatcgtcagt tcgcggcgcg tgtcggcgtc gcggagcgtg acatggcggg gatcggacga
     2161 gtcgtttgcc agcgccaacc agacgccggc gtcacgatcg ctggctggct gaacgccgaa
     2221 ctgccactcg gcggtctcgc gcatgagcgg tatcgcgtac gacccacccg gcagccagcc
     2281 caccgcgccg gcaacgatcg gcgctgacgc ggtgacagag acggcgacac cattctgaag
     2341 atcgacatcg agcgcaacgg tcgcgccagg ttggaggctg acactggtcg cgcgcaggag
     2401 cgtgtcgctc tcagtgagca ggcgaatact ggcgtcaacc ggattggagg ttgggttgga
     2461 gagcgtgagg cgagctgtcg cttccagggt ggcgaagcga tcgcgcggga gtgggccgag
     2521 cagccagttc gtcgcggccg gctgtgcggg caggatgcgg agtccggacg cgctgtcgat
     2581 caactcggcg atgacgtcag cgtctgcggc gatcgtgatc gcgccaacga agtgttcgtc
     2641 gcatgtggcg cggtcgagcg gtggttgcaa ctggatgatg tgaccgtcga gcgaggcagt
     2701 ttcctgccag agcaggcggc cggtggcgtc cagcaggtgg atagtggcgc tggttgggac
     2761 atccgatgca gcccgaagca ccagcgcatc caggttgcag gggccgctga tcgggatcgc
     2821 gagtgatgcc gccggggtca acgcaggcag cgcttcccag ttaccgccct cgtcggcgat
     2881 cgccggcgct gcggtgggcg atggggaaac gtcgctgccc tc
//
LOCUS       DASUGJ010000688         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1780308, whole genome shotgun sequence.
ACCESSION   DASUGJ010000688 DASUGJ010000000
VERSION     DASUGJ010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780308"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..537
                     /locus_tag="VFV93_15610"
     CDS             25..537
                     /locus_tag="VFV93_15610"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5166832.1"
                     /translation="MAMPDDGYRYELVRGELVRLPMSAHQSSRIAIRIANRIGPFVEA
                     GNLGNIAGADGAYIFARDPYTVRIPDLSFVRAERLPPPDAWVKFLELAPDLAVEVVSP
                     SDSANEIQDKVNEYLDVGVRLIWVVYPIQHTITVYSADRIGRVLTDADTLDGGDVLPG
                     FSLPVAEIFA"
     gene            complement(548..790)
                     /locus_tag="VFV93_15615"
     CDS             complement(548..790)
                     /locus_tag="VFV93_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166833.1"
                     /translation="MVAQPSDPANALLALDVTVQIRGAFGERQVPPADLFAVPAAERV
                     LNGSAPDAATFVRAADAVLADPRGAGVAGGSTSSAG"
     gene            921..>2764
                     /locus_tag="VFV93_15620"
     CDS             921..>2764
                     /locus_tag="VFV93_15620"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5166834.1"
                     /translation="MDETEHSRLADSGAHEAESTSRSTPFMLETREQLRAILQGVTDG
                     VVVQDSDGHIRYVNEAAAQLFSTSDPGGLLGRTVSDAFAQFDILDGSGLPITINDLPA
                     REVFGGAPTARRSIVRRDRLTGEERWWIITASPVSSDTDTVPYVVCIIRDATDRVRAI
                     RANARLATIVESSADAIVGKTLDAIVTSWNPAAERLYGYTAREMVGRSVALLIPPDRP
                     DELAGIMARVRAGEQLEPHETIRVRKDGRRVDVALSISPIRDQDGRITGASTIARDIT
                     GLRRSQELMQLLAEAGDVLGTSLDYRATLDRLATLLVPRLADSCSINMVDASGIPTRL
                     AHAHADPAKIEVLRQLEDLVPPEPAEAEALMQAVSNGEPLVFRELADDFLLANARSSE
                     HLELLRQLRYRSVIVVPMMARGSVVGTISLVYAESDRRYQDDEIELAREIARRAALAV
                     DNAILYADAQQAARAREDFLLTASHELRTPLTSVKASAQLIARYLDQPQPDHGRIETM
                     VARLQTEIGRLEALSVDLLDAARIQRGRFEMHLEPVDLVTIADEVLASLERSSFRRSS
                     HRLVLDASEPVSGIWDAQRLQHVVTNLVSNALKYSPDGGEVCVRIRQDG"
BASE COUNT          495 a          955 c          866 g          448 t
ORIGIN      
        1 cggctcctca cggccgacga cttgatggcg atgccggacg atggctatcg ctacgaactc
       61 gtgcgtggag agctggtacg actgccgatg tccgctcatc aaagtagccg aatcgcgatt
      121 cggatcgcaa atcggatcgg acctttcgtt gaagcgggca atctcggcaa tatcgcaggg
      181 gcggacgggg cctacatctt tgcgcgtgac ccctataccg tccgcattcc tgatctgtcg
      241 ttcgttcgag cggaacgcct gccacctcca gacgcctggg tgaagttcct tgaactcgca
      301 cccgatctcg cagttgaggt cgtctcaccg tccgatagtg caaacgagat acaggataag
      361 gtgaacgaat acctcgacgt cggtgtccgc ctgatctggg ttgtctatcc gattcagcac
      421 accataacgg tctactccgc cgacaggata ggccgcgtcc tcactgacgc agacaccctc
      481 gacggcggcg atgttctgcc aggcttcagc ctcccggtcg cggagatctt cgcgtagcgc
      541 aatcgatcta cccggccgac gatgtgctac cgccagcgac tccagcgccc cgcggatcag
      601 cgaggacagc gtcggcagcg cggacgaagg ttgcggcgtc cggagcggag ccgttcagca
      661 cccgctcggc ggccggcact gcgaagagat cggccggcgg aacctgacgc tccccgaacg
      721 cgccgcgaat ttggaccgtc acgtccagcg ccagcagcgc gttggccggg tcggacggtt
      781 gagccaccac gcagggggaa tcgtcgaaga gagccggaat gggtaccagc gtcgtcttgc
      841 gcggcctcag ctgacgccgc atcatgatga ctgggtagac ttggtggacg actgccggat
      901 gacgaaccaa aggcaggaac gtggacgaaa ctgagcacag ccggttggcc gacagcgggg
      961 cacatgaggc ggagtcgacc agccggagca ctccgttcat gctcgaaaca cgagaacagc
     1021 tcagggcgat tctgcaaggc gtcaccgacg gcgtcgtcgt tcaggacagc gacggccaca
     1081 tccgctacgt caacgaagca gctgcccaac tgttctcaac gtcggaccca ggcggcctgc
     1141 tgggccggac cgtctctgac gcgttcgccc agttcgacat cctcgacggc agcggcctgc
     1201 cgatcacgat caacgacctg cccgcacgcg aggtgttcgg cggcgccccg acagcgcgcc
     1261 gttcgatcgt ccgccgcgat cggctaacgg gcgaggagcg ctggtggatc atcaccgcct
     1321 cgccggtttc gtccgacacg gacacggttc cctacgtcgt ctgcatcatc cgcgacgcga
     1381 ccgaccgggt tcgtgccatc cgcgccaacg cccggctggc gacgattgtc gaatcctcgg
     1441 ccgatgccat cgtcggcaaa acgctcgacg cgatcgtcac cagctggaac ccggccgccg
     1501 aacggctcta cggctacacc gcgcgggaga tggtcggccg ctcggtcgcg ctcctgatcc
     1561 cgccggaccg gccagacgaa ctggccggca tcatggcgcg ggtacgggcc ggcgagcaac
     1621 ttgagccgca cgagacgatc cgcgttcgca aggacggccg gcgggtcgat gtcgccctct
     1681 cgatctcgcc gatccgcgac caggacggcc gcatcaccgg cgcatcgacg atcgcccgcg
     1741 acatcacggg cctgcgccgc agccaggagc tgatgcagct gctggccgaa gcgggtgacg
     1801 ttctcggcac gtcgctcgac taccgggcca cgctggatcg tcttgcaacg ctgctcgtgc
     1861 cacgcctcgc cgattcatgc tcgatcaaca tggttgatgc atccggtatc ccaacccggc
     1921 tggcgcacgc gcatgccgac ccggccaaaa tcgaggtgct gcgccagctg gaggatctgg
     1981 tcccgccgga accggccgag gcggaagcgc tgatgcaggc cgtcagcaat ggcgagccgc
     2041 tcgtctttcg agaactggcc gacgacttcc tgctcgccaa cgccagatca tccgagcatc
     2101 tcgaactcct gcgccaactc aggtaccgct cggtgatcgt cgttccgatg atggcgcgtg
     2161 gcagcgtggt cggcacgatc tcgctcgtct atgccgaatc ggatcgccgc taccaggacg
     2221 acgagatcga gctggccagg gagatcgccc gccgggccgc gctcgccgtc gacaacgcga
     2281 tcctctacgc cgacgcccag caggccgccc gggcgcgcga ggacttcctg ctgaccgcct
     2341 cccacgaact gcggacccca ctcacgtcgg tcaaggcctc tgcccagctg attgcccgct
     2401 atctcgatca gccccagccc gaccatggcc ggatcgaaac gatggtcgcc cgcctgcaaa
     2461 cggagattgg ccggctggaa gccctctcgg tcgatctgct cgatgccgcc cggatccagc
     2521 gcggtcgctt cgagatgcat ctggagccgg tcgatctcgt cacgatcgcc gacgaggtgc
     2581 tcgcgtcgct ggagcgctcc agcttccggc gctccagcca tcgactcgtg ctcgacgcca
     2641 gcgaaccggt cagcggcatc tgggacgcgc aacgcctgca acacgtcgtg accaacctcg
     2701 tctcgaacgc cctcaagtac tcgcccgacg gcggcgaggt ctgcgttcgg attcggcagg
     2761 atgg
//
LOCUS       DASUGJ010000689         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1084935, whole genome shotgun sequence.
ACCESSION   DASUGJ010000689 DASUGJ010000000
VERSION     DASUGJ010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084935"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VFV93_15625"
     CDS             <1..1193
                     /locus_tag="VFV93_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX5166835.1"
                     /translation="LPAERQTALFSATLPKRITDLATRYLDAPVTIRIEPEQVSVPQI
                     TQTYYEVVQRAKLEALTRILDLAEPTSAMIFCRTKIETDEVTQALQGRGYAAEAIHGD
                     LSQMMRDRVMNRFRAGTATLLVATDVAARGLDVEHVSHVINYSLPGDPESYVHRIGRT
                     GRAGRTGEAISLVTPRERRLLRWIERAVGQRMEPRRVPTASDVAQAQRERLAATIGEI
                     IEMQELEHPMSLVDELAAYYDPSQIAAAAISLLIEPEPEEPDIPVTAFGAERGMLRLF
                     LNIGSKDGLRPSDVVGAIANEAQIPGKSIGVIEIKDTYSFVEIPDHLAEKVVNAIQGS
                     RIRNREVRVEIARPRDGSTPSGNRQAGDRRDGQSRDGRPPRDRDSRPRREGSRPRRER
                     DRSR"
     gene            complement(1219..1443)
                     /locus_tag="VFV93_15630"
     CDS             complement(1219..1443)
                     /locus_tag="VFV93_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166836.1"
                     /translation="MPRLPRNRGRSRRRGRRDPFDRSIIPIYPSLRPIGFFAGVMTRW
                     LMRPSRLGISRGLLGLIVVVVLIWLVVLWS"
     gene            complement(1536..1796)
                     /locus_tag="VFV93_15635"
     CDS             complement(1536..1796)
                     /locus_tag="VFV93_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166837.1"
                     /translation="MHEVFPVVAGILLGFAAFRIESMRLRAIVIAVMSIALGVAATYI
                     SGEALISWAFVLIDIPLVLVSAIGALVASYAWQRRGATQAPM"
     gene            1980..2210
                     /locus_tag="VFV93_15640"
     CDS             1980..2210
                     /locus_tag="VFV93_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166838.1"
                     /translation="MSHETVALLLWSMLAIAIALTIVGVGIHSSRVLFVAAALSLVFG
                     LAAIFSIGIFILALALIQLSLGIAYRRQSSAA"
BASE COUNT          429 a          804 c          738 g          373 t
ORIGIN      
        1 ccctgccggc cgagcggcag acggccctct tctcggcaac gctgccgaag cggatcaccg
       61 acctggccac gcggtatctg gatgccccgg tcacgatccg gatcgagccg gagcaggtca
      121 gcgtcccgca gatcacgcag acctactacg aggtcgtcca gcgcgccaag ctggaggcgc
      181 tgacacgcat cctcgacctg gccgagccga cctcagcgat gatcttctgc cggacgaaga
      241 tcgagaccga cgaggtcacc caggcgctcc agggacgtgg ctacgccgcc gaggcgatcc
      301 acggcgacct ctcacagatg atgcgcgacc gcgtcatgaa ccgcttccgg gccgggacgg
      361 cgacgctact ggtcgcgacc gacgtggccg cgcgagggct ggatgtcgag catgtctcgc
      421 acgtcatcaa ctactcgctg ccaggcgatc cggagagtta tgtccaccgc atcggtcgga
      481 ccggccgcgc cggccggacc ggcgaggcca tctcgctggt gacgccccgc gaacggcggc
      541 tgctgcgctg gatcgagcgg gccgtcggcc agcggatgga gccacggcga gtcccgactg
      601 cctcggacgt tgcccaggcc caacgcgagc ggctggccgc cacgatcggc gagatcatcg
      661 agatgcagga gctggagcac ccgatgtcgt tggtcgatga gctggccgcc tactacgacc
      721 cgtcgcagat cgcggcggct gccatctcgc tgctgatcga gccggagccg gaggagccgg
      781 acatcccggt caccgccttc ggcgccgagc ggggcatgtt gcggctgttc ctcaacatcg
      841 gcagcaagga cgggctacgc cccagcgatg tcgtcggcgc gatcgccaac gaggcgcaga
      901 tccccggcaa gtcgatcggc gtgatcgaga tcaaggacac ctactcgttc gtcgaaatcc
      961 cggatcacct cgccgagaag gtcgtcaacg ccatccaggg ctcgcgcatt cgcaatcgcg
     1021 aggtgcgcgt cgagatcgcc cgcccgcgcg acggctcgac cccgtccggc aaccgccagg
     1081 ccggcgaccg ccgcgacggc cagtcccgcg atggccgccc gccccgcgac cgcgactccc
     1141 gcccccgccg cgaaggcagc cgtccccgcc gcgaacggga ccggagccgc tagtttcgaa
     1201 cgccaggtga agtgcgcatc atgaccagag cacgaccagc cagatcagga cgacgaccac
     1261 gatcaggccg agcaacccgc gactgatacc caaccggctt ggccgcatca gccagcgtgt
     1321 catgacgccg gcgaagaagc cgatcggacg cagggacgga tagatcggaa tgatgctccg
     1381 gtcgaacggg tctcgccgtc cccgccgacg gctccggcct cgattcctgg gcagcctcgg
     1441 catacgtcaa tgtctcccca tcatgttgac tctttctcga cacagcaacg cccgccccgg
     1501 ggtcggtggg cgggcgtttg cgggcagtgt gcgcgttaca tgggtgcctg ggtcgcgccg
     1561 cggcgctgcc acgcgtagct ggcgaccagc gcgccgatgg ccgagacgag caccagcgga
     1621 atatcgatca ggacgaacgc ccaactgatc agcgcctcgc cactgatgta ggtggcggcg
     1681 acgccgagcg cgatgctcat gacagcgatc acaatcgctc gaaggcgcat cgactcgatt
     1741 cggaaggctg cgaaccccaa cagaatcccc gcaacgaccg ggaagacctc gtgcatgatg
     1801 tgctcccttc gatcctctcg atgaacatct ggccccgaag catggaatag caccacttca
     1861 cgaatctgtc tacggttggg tgagacgcgt gacgagtaat gagaaatcgg tgacacacag
     1921 aatccgtcgt cggtgttcat ttattgagcg ctgggttcgg tcagacagga gggttgtcga
     1981 tgtcgcacga aacagtggcg ctgctactct ggtcgatgct cgccatagca atcgcgctca
     2041 cgatcgtcgg cgtcggtatc cattcctcgc gcgtgctgtt tgttgcagcg gcgctctcgc
     2101 tcgtgttcgg cctggccgcc atcttcagta tcggcatctt catccttgcg ctggcgctga
     2161 tccagctttc gcttggcatc gcctatcgac gacaatcatc ggcggcgtaa cacgtaatgc
     2221 gccccagatg aacggcctcc acgagtaccc cgtcgccctt gccctgtcat cctgagccgc
     2281 agcgaaggat cgcctggcgt aaatgacacg aggacgtctt tcggcagacg aacgttcaca
     2341 tcct
//
LOCUS       DASUGJ010000690         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1917921, whole genome shotgun sequence.
ACCESSION   DASUGJ010000690 DASUGJ010000000
VERSION     DASUGJ010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917921"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VFV93_15645"
     CDS             complement(<1..1200)
                     /locus_tag="VFV93_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5166839.1"
                     /translation="MTDNRLLRNGFVWIILIIAVIAVWFMITQGGNDPRQVDLGEVAT
                     AIDAGSVERLEQREDSRQVIVHFKGDESPAAFRLPPDVDIYQALEQYGVNASQVTIEI
                     TRSSQWGAWIGALSIFLPFLLLIGIVVFMMRQAQGSNNQAMSFGKSRARMFTGNKPTV
                     TFADVAGVNEAKEELAEVVEFLKYPEKFSALGARIPRGVLLVGPPGTGKTLLSRAVAG
                     EAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNAPCIVFVDEIDAVGRQRGAGLG
                     GSHDEREQTLNQILVEMDGFDTSTNVIVIAATNRPDVLDPALLRPGRFDRQVVLDRPD
                     ISGRLAVLEVHSKGKPFEDDVKLDELARQTPGFSGADLENLVNEAAILAARRNKKTVG
                     RLELAEAI"
     gene            complement(1330..1878)
                     /gene="hpt"
                     /locus_tag="VFV93_15650"
     CDS             complement(1330..1878)
                     /gene="hpt"
                     /locus_tag="VFV93_15650"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX5166840.1"
                     /translation="MTEAANVELPAGVERILLTEQQVQQRIRELGAELAEHYDDRKPL
                     LVGVLTGAYVFMADLSRAMNIELDVEFMAVSSYGLATQTSGVVRILKDLDMPVEGKEV
                     LIVEDIIDSGLTLNYLLDVLRRRNPKDLRVVSLLRKKRDDAHAVQVDYLGFEIPDEFV
                     VGCGLDAGRRFRNLPYVAVVRE"
     gene            complement(1880..2893)
                     /gene="tilS"
                     /locus_tag="VFV93_15655"
     CDS             complement(1880..2893)
                     /gene="tilS"
                     /locus_tag="VFV93_15655"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5166841.1"
                     /translation="MASADRSIRRHPAYRAVVRRWPEKGLLACSGGVDSSALLALAGT
                     AVQRGDLPPFVVVHVDHLTRDEGAAEGEVVEALAMRFGLQTVRTTVDAADAEPSSSSP
                     EDRLRRQRYAVLARVAVERGSPAVVTAHTRDDQIETILMRLLTGAGGIATAGMQERSV
                     LATAAGEITIHRPLLDTSRADLLGVLDRLGIEPLHDPTNADRGYRRNALRLDIIPAIR
                     LAYPGFEAALARSVGLAAQDSQALDGWAERVAKSTLTSDDASIRVERAALREVPAAVA
                     SRIIRMAASRMMPDNPRELTFERVESIRLATSGRTGAMIELPYGVLARIERHEIVFER
                     RNH"
     gene            complement(2986..3057)
                     /locus_tag="VFV93_15660"
     tRNA            complement(2986..3057)
                     /locus_tag="VFV93_15660"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3023..3025),aa:Met,seq:cat)
     gene            3203..>3597
                     /locus_tag="VFV93_15665"
     CDS             3203..>3597
                     /locus_tag="VFV93_15665"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5166842.1"
                     /translation="MVSAPPDTIESPLLTGLNPEQRQAVTTTDGPVLIVAGPGSGKTR
                     VLTVRIAYLIQERGVAPWNIMAVTFTNKAAREMRERVERLVGERAKWISLGTFHAFCA
                     RVLRQYGSAIEIDPRFVIYDDGDQMSAVK"
BASE COUNT          670 a         1220 c         1077 g          630 t
ORIGIN      
        1 gatcgcctca gcgagctcga gtcgcccgac cgtcttcttg ttgcgccggg cggcaaggat
       61 cgccgcctcg ttgacgagat tttcaaggtc tgcgccggag aagcccggtg tctgtcgcgc
      121 caactcgtcc agcttgacat cgtcctcgaa cggcttgccc ttcgagtgga cctccagaac
      181 cgccagccgg ccgctgatgt cgggccggtc gaggacgacc tgccggtcga agcgccccgg
      241 tcgcagcagg gccggatcga gcacgtcggg acggttagtg gcagcaatga cgatcacgtt
      301 cgtgctggtg tcgaagccat ccatctcgac caggatctgg ttgagggtct gctcacgctc
      361 gtcgtggctg ccgccgaggc ccgcgccacg ttggcgaccg acggcatcga tctcgtcaac
      421 gaagacgatg cagggcgcat tgcgcttggc ctgatcgaag aggtcacgaa cgcggctcgc
      481 gccgacgccg acgaacatct caacaaactc cgaaccggag atgctgaaaa acggcacgcc
      541 agcctcgccg gccaccgccc gcgacagcag cgtcttacct gttcccggag ggccgaccag
      601 caggacgcca cgcggaatgc gcgcaccgag ggcagaaaac ttctctggat acttgaggaa
      661 ctcgacgact tcggccagtt cttccttggc ctcgttgacg cctgcgacat cggcgaacgt
      721 caccgtcggc ttgttgccgg tgaacatccg cgcccggctc ttgccgaacg acatcgcctg
      781 gttgttgctg ccctgcgcct gccgcatcat gaagacgacg atgccgatca gtagcaggaa
      841 cggcaggaaa atgctcaacg cgccgatcca ggcgccccac tgactcgaac gcgtgatttc
      901 gatcgtgacc tggctggcat tcacgccata ctgttcaagc gcctggtaga tgtccacatc
      961 cggcgggagc cggaacgcgg ccgggctctc gtcgcccttg aagtgaacga tgacctggcg
     1021 cgaatcctcg cgctgctcaa gtcgctcgac gctaccggcg tcgatggccg tggccacctc
     1081 acccagatca acctggcgcg ggtcgttgcc gccctgggtg atcatgaacc agaccgcaat
     1141 gacggcaatg atcaatatga tccagacgaa gccattgcgg agtaatctat tgtcggtcat
     1201 ctggtgtcct ccgtgtgcgc ttcgcgaccg tctccggggc acaaatcggg agcgtcggcc
     1261 atgtggaagc gacgttccac gattatagca ggtgagtcat atcctcccgc gcacgttgcg
     1321 cgaccatcgt tattctcgga caaccgccac atacggcagg ttacggaagc gccgtcccgc
     1381 gtccagcccg caaccaacca cgaactcatc cgggatctcg aaaccgaggt agtcgacctg
     1441 gacggcgtgt gcgtcgtcgc gcttcttccg caacagtgaa acgacccgca agtccttcgg
     1501 gttccgtcgt cgcagaacat ccagcaggta gttcaacgtc agaccgctgt cgatgatgtc
     1561 ctcgacgatc agcacttctt tgccctctac cggcatgtcc aggtccttga gaatgcgcac
     1621 gacaccgctc gtctgcgtcg ccagtccgta gctggagacg gccatgaact cgacatccag
     1681 ctcgatgttc atcgcccggc tcaggtcggc catgaagacg taggcgccgg tcaggacacc
     1741 gaccagcaac ggcttgcggt cgtcgtagtg ctccgccagt tcagccccca gctcccgaat
     1801 tcgttgctgc acctgttgct cggtcagcag gatccgctcg acaccggcag gtagttccac
     1861 gttcgcagct tcggtcacgc taatggttcc ttcgttcaaa cacgatctcg tggcgctcga
     1921 tccgggccag cacgccatat ggcaactcga tcatcgcgcc ggtcctgccc gatgtcgcca
     1981 gccgaatgct ctcaacccgc tcgaacgtca actcacgggg attgtcgggc atcatccggc
     2041 tggctgccat ccgaatgatc cgcgatgcca ccgccgccgg tacctcccgc agcgccgcgc
     2101 gctcgacgcg gatcgaagcg tcgtcgctcg tcagcgtcga tttcgccact cgctccgccc
     2161 agccatcgag cgcctgggag tcctgtgccg ccaacccgac cgaacgcgcc aatgccgctt
     2221 caaagcccgg atacgccagc cggatcgcgg ggatgatgtc cagccgcagc gcattgcgac
     2281 ggtagccgcg atcggcgttg gtcggatcgt gcagcggctc gatcccgagc cggtcgagaa
     2341 cgcccagcaa gtcggctcgt gacgtatcga gcagcggacg atggatggtg atctctccgg
     2401 ccgcagtcgc cagaaccgat cgttcctgca tcccggcggt tgcgattcca cctgcgccgg
     2461 tcagcagccg catcaggatc gtctcgatct gatcgtcacg cgtgtgagcc gtcacgacag
     2521 cgggcgaacc tcgttcgaca gcgacccgag caagcacggc gtagcgctgc ctgcgcagtc
     2581 gatcctccgg cgacgaactt gatggctcgg catcagcagc atccaccgtt gttcgcacgg
     2641 tttgcagtcc gaagcgcatc gccagcgcct cgaccacctc gccttcggcg gcgccctcgt
     2701 cccgtgtcag atgatcgaca tggacgacga cgaacggcgg gaggtcgccg cgctggacag
     2761 ccgtcccggc cagcgccagc aacgccgacg agtcgacgcc gccggagcag gccagcagcc
     2821 ctttctccgg ccaacgtctg acgactgcac gataggcagg gtgacgccgg atcgaacggt
     2881 cagcagatgc cacgactggc gcctctccgc ggagcagcgg catgaatcga aggacaaaaa
     2941 atgagaccgc gtggccgatc tggcccagcg gtctcatcac gtcggtgccg gtggacggat
     3001 ttgaaccatc gaccaagggc ttatgagtcc cctgctctac cactgagcta caccggcatt
     3061 tgtagcctgg taatgatagc gaaacgtggc ctggctggca acttctgtcc cgccagtcgg
     3121 ggcttgagta caatcagcgt acgtacttgc cagcacccct cagtccgctc cgcgacgccc
     3181 gccgacgtgt aacgaggtga ccatggtttc cgctccgcca gacacgatcg aatcgccgct
     3241 cctgaccgga ctgaaccccg aacagcgaca ggccgtcaca acaaccgacg ggccggtctt
     3301 gatcgtcgcc ggccccggca gcggcaagac ccgtgtcctg acggtgcgga tcgcctatct
     3361 gattcaggag cggggcgtcg caccctggaa catcatggcc gtcacattca ccaacaaggc
     3421 cgcacgcgag atgcgcgaac gggtcgagcg gctggtcggt gagcgggcga agtggatctc
     3481 gctcggcacg ttccacgcct tctgcgcccg cgtcctgcgc cagtacggca gcgcgatcga
     3541 gatcgacccg cgcttcgtga tctacgacga cggcgatcag atgagcgccg tcaagca
//
LOCUS       DASUGJ010000691         7923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_806855, whole genome shotgun sequence.
ACCESSION   DASUGJ010000691 DASUGJ010000000
VERSION     DASUGJ010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806855"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFV93_15670"
     CDS             <1..315
                     /locus_tag="VFV93_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166843.1"
                     /translation="EGGRLLWTDHELANGVREGARLAMVRGSESSSPITTTTPIKTHI
                     LDKTAGLNSGDLTVSAVGLGGDPGESVTVTGNYQYDVLFGIIPGLSDFTMTESSTVII
                     QH"
     gene            complement(387..920)
                     /locus_tag="VFV93_15675"
     CDS             complement(387..920)
                     /locus_tag="VFV93_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166844.1"
                     /translation="MLDASGLYRIRYGDIFRAVGHFLDTSLFRDLTLVETQDGFIVKG
                     GTIRTGDTGTGFVPQSYLFTDQDLDTLLDQAIERRGGEQSVESMLPSITVDGERVRYE
                     DALRSIGLLVDRIGWHEVVLIQTSLGFHLKGVSKEAPIDRLIDAPALKGLLEEMRNSR
                     EVQAQAQESPRRRLWPR"
     gene            1233..2516
                     /locus_tag="VFV93_15680"
     CDS             1233..2516
                     /locus_tag="VFV93_15680"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HEX5166845.1"
                     /translation="MHWGRLRFTLTRWRNRSAADGQVFVIFAVAALVLIGFVALSVDA
                     GYLMAERRQAQNAADAGALAGAKSVQRSQQLQAETSAIDYVEANGFTGSGDSIDAVYP
                     ADPAPPSPYLPAECVFVEVTHDVQKFFVGALYTGDWKVGADAWACTKLKPEPYALITL
                     DPDGDGIKSGGNSDLTIVGGGAMSNSDADICGTASWISADGPLDAYSNVTICSNASVD
                     ANPIRSNSPAIDDPLSAIPEPTMAQCGANQPDPNIGNSSPASTTISPGKYTNGITVNA
                     KDKELILSPGLYCFGQDFKANAGSTGVTIKGSNVTLYFYGSSMLNIDGGGVKMLIDSP
                     PGGTCSLAACNAEIVVFYSRTNCAEMRLVGGNGTDMEGIFYAPCSLLHLGGGSGSSYT
                     GQIIVAEAEILGGSAITLIYDNKVATEVPRVFLVE"
     gene            2770..4011
                     /locus_tag="VFV93_15685"
     CDS             2770..4011
                     /locus_tag="VFV93_15685"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX5166846.1"
                     /translation="MKKRIVEMRVRPDIPLRLPGPVRHAGQGQVMILFALASLVLIGF
                     VALSIDTGFLMSERRQTQNAADAAALAAAKSLFDGKSGQMVAAAKAYGADNADVSQDN
                     VTVSWPPASGEHAGDNQYVQVTVTKDVEKFFVGALYTGDWKVSASAVAGIEPQGANYA
                     LITLDRTHNPGIYMNGTTTIRLIGDHASAYGSTDVDGNGKLYVDGAVDSHGSVSGVGS
                     APDGIHTNMPYIPDPLDGVPAPSKPATVRTGCTGDCDLQPGYYKNQTILCKWVCNFAP
                     GLYYFENTSVKSQSTKSEMNGPGGVMFYFTGSSEFDSKNGGVNLRAPAASPYAGGQEG
                     VVFWSASCNEINFQGNGDFYMHGIVYAPCADVSMNGTPGGDGVKGQWIVNTFKVVGTS
                     DFRLNYHSYVDTKRPKIFLVG"
     gene            4178..4957
                     /locus_tag="VFV93_15690"
     CDS             4178..4957
                     /locus_tag="VFV93_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5166847.1"
                     /translation="MQITRYRRDGQDRVGVIENNIVRETRGDMFGKLSIGPEIAPVGE
                     MELLPPVVPSKLLAIGLNYQDHISQDAPGFETPENPIVFLKPPSALIGHGGQIVLPPG
                     LEKIDAEAELAVVIGRECRHVKAARAWDVVLGLCPSNDVSARDYQFKDGQWSRAKGFD
                     TFAPLGPAIVTGVTAEDLAITCRINGEIRQQSSTKHLIFDIPYLIEFLSRVMTLLPGD
                     VIMTGTPAGPPKLAPGDVCEVEIAQLGVLRNTCVAEPDLPA"
     gene            4954..5481
                     /locus_tag="VFV93_15695"
     CDS             4954..5481
                     /locus_tag="VFV93_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5166848.1"
                     /translation="MTMPAAGLHLRPATLDDVPRLKELGVLGWETTYHDYVSPENRAA
                     YLAGPFWSLERLAAVVDDPASLTLVATTGAEPAAGFLTIEPVETSVVELTRLYVDPAL
                     RGLGAGAALFDAARGWSRDHGARTILVNVFADNTGGRRFYERAGCTLIRLEPTTVGDQ
                     TVADAWYECEVLGSR"
     gene            5686..6633
                     /locus_tag="VFV93_15700"
     CDS             5686..6633
                     /locus_tag="VFV93_15700"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5166849.1"
                     /translation="MQPVTTGRKHGRQLRLLLLATLVVSLVTLSLPATAVEAPSPPEV
                     FAENAVVLSDFNEVLYDHNAHQRTAMASTTKIMTAILAVRYGNLDQDVLIDESDIVGE
                     ASMGLYAGETVKLRDLLYGLLLPSGNDAAHAIARTVGWKPGTTTADEAVNNFVGLMNQ
                     TASEYQLRDTHFMNPHGLDAWGHYSTPFDLAIMLRAALNYDVIRETMQTLSINAGGHD
                     LWNHNQLYGMRDDMIGGKTGYTEAANFCLAAAAGRNGRFVIAVVTRDDGDNWVYDVSQ
                     LLDWGLIAVDRQGIPPGVNPNLIGTQLNQPDPVIQQGSP"
     gene            6678..7247
                     /locus_tag="VFV93_15705"
     CDS             6678..7247
                     /locus_tag="VFV93_15705"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5166850.1"
                     /translation="MNSVAKRTPLLSVEDYLELEASSSIRHEYIDGHIYALAGVTNRH
                     DDIVLNIMARFHAAARGTECRVRSGEVQLRVSASRYYYPDIHVVCHPISSEARSVSDP
                     CVIVEVLSPSTEQVDQREKLFAYTTIQTLRAYYIVHQDRMTVENHWCDDEGTWWHGTL
                     HGGGALKVPCLNVDLPLEQIYEGVTFEQQ"
     gene            7299..7865
                     /locus_tag="VFV93_15710"
     CDS             7299..7865
                     /locus_tag="VFV93_15710"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5166851.1"
                     /translation="MSSMAHRREPLTTAEYLELESRSPVRHEFIDGFLYALAGVSNRH
                     DRIVVNLIVSFGSRARGTDCQVHTGEMMVRVSPTRYYYPDVHVTCQPETDDSRTITAP
                     CVVVEVLSPSTTGTDRREKLCAYRQIETLRSYLIVHADEKVIEQHWIDNGVWRHDILE
                     DNASVHIPCLDFDLDLADIYEGVTFEES"
BASE COUNT         1592 a         2606 c         2300 g         1425 t
ORIGIN      
        1 gaaggcggcc ggctgctctg gaccgatcac gaactcgcca acggagtccg cgaaggcgcg
       61 cggctggcga tggtccgagg ctcggagtcg tcctcaccaa ttaccacgac cacgccgatc
      121 aagacgcaca ttctcgacaa gacagcggga ctgaacagtg gtgacctgac cgttagcgct
      181 gtcggcctcg gtggcgatcc cggcgagagc gtgaccgtca cgggcaacta ccagtatgat
      241 gttctcttcg gcattattcc cggactgtcg gacttcacga tgaccgaatc cagtaccgtc
      301 atcatccagc actgacacca caagcaggca aacgtgaagg gcggccggca actggccgcc
      361 cttcacgttt gccgtatagc cctgaactac cgcggccaga gccgtcgacg gggtgactcc
      421 tgagcctgtg cctggacctc gcggctattg cgcatctcct ccaacagacc cttcagggcg
      481 ggagcgtcga tcaacctgtc gatcggtgct tccttcgaga cgcccttgag atggaagccg
      541 agcgaggtct ggatcagcac gacctcgtgc cagccgatcc ggtcgaccag caggccgatg
      601 ctgcgcagcg cgtcctcgta acggacacgc tcgccgtcga ccgtgattga gggcagcatc
      661 gactcgaccg attgctcgcc accgcgccgc tcgatcgcct ggtcgagcag cgtatcgaga
      721 tcctgatcgg tgaacagata tgactgcggg acaaagcccg tcccggtatc gccggtccga
      781 atcgtcccac ccttgacgat gaagccatcc tgtgtctcaa ccagcgtcag gtcgcggaac
      841 agcgatgtat ccagaaaatg accgacggca cggaagatgt ctccgtaccg aatgcgatag
      901 aggccactcg cgtcaagcac atgtaccttc tttcggcgct acgtcatcct gttcgtcgga
      961 cgcgttgata ccaaactgct tcgcgccagt cgtgatcctt gagccgcaag tctagcaaag
     1021 cacgatgaag cttcggtcac gttatgggct agcctgaacc tacctgttcg ggaccgggta
     1081 ccggcagaaa acatgacttt cgcgtcagtg aggtatcccg ccaacgaacc atacaatcga
     1141 ccgtattgat gacctgcaga ctaacgcgcc gcatggccgg atcgcggcat gcggcatctg
     1201 gattgttcac gtgtacgcta ggacggcaca tcatgcactg gggtcgcctt cgattcacac
     1261 tcacccgctg gcgaaaccga tccgcagcag acggccaggt ctttgtgatc ttcgccgtcg
     1321 ccgcgctggt cctgatcggc ttcgtcgccc tctcggtcga cgccggatac ctgatggccg
     1381 aacgacgaca ggcgcagaac gcggccgacg ccggggcact ggccggcgcg aaatctgtcc
     1441 agcgcagcca gcagctacag gcggagacga gcgccattga ttatgtcgag gcaaacggct
     1501 tcaccggcag cggcgactcg atcgacgccg tctatccggc cgatcccgct ccaccaagcc
     1561 catacctgcc cgctgaatgc gtcttcgtcg aagtcaccca cgatgttcag aagttcttcg
     1621 ttggcgcgct ctacaccggc gattggaaag tcggggccga cgcctgggcg tgtaccaagc
     1681 tgaagccgga accatatgcg ctgattacgc tcgatcccga cggcgatggc atcaaatccg
     1741 gtggcaattc ggacctgacg atcgtcggcg gcggcgcgat gtctaactcg gacgccgaca
     1801 tctgcggcac ggccagctgg atctcggccg acggcccgct ggacgcctac agcaacgtca
     1861 cgatctgctc gaacgcgtcc gtcgatgcga acccgatccg ctccaattcc cccgcgatcg
     1921 acgatccgct gagcgccatt ccggaaccaa cgatggcgca gtgcggcgct aaccagcccg
     1981 atccgaacat cgggaactcg tcgccggcct caacgacgat ctcaccgggc aagtacacca
     2041 atgggatcac tgtcaacgcc aaggacaagg agttgattct gtccccgggc ctctactgct
     2101 tcgggcagga cttcaaggcc aacgcgggct cgactggcgt gacgatcaag ggctcgaacg
     2161 tcacactcta tttctacggc agctcaatgc tcaatatcga cggcggcggc gtcaagatgc
     2221 tcatcgattc accgccaggc ggcacctgct cgctggcagc ctgcaacgcg gagattgtcg
     2281 tcttctattc gcggacgaac tgtgccgaga tgcggctggt cggtggcaac ggcaccgata
     2341 tggagggcat cttctacgct ccgtgttcac tcctccattt gggcggcggt tccggcagca
     2401 gctacaccgg ccagatcatc gtcgccgagg cagagatcct cggtggctcg gccatcacgc
     2461 tcatctacga caacaaagtc gcaaccgaag ttccgcgggt cttcctcgtc gagtaggcca
     2521 atgcaccggc gcggagcaaa tcccctggcg ggggcgagtc aactactcgc ccccgccggt
     2581 caacctgtgc aatctgcgct acggtaacct gtggcatccc ccaaagttcg cacaacgtac
     2641 caatcgtttc atgactgatg taccttcagg aatagcccgt catctgacta tattcatcac
     2701 aggcaatctc atattgccca accgttgtaa ttccacgcgg aacgctgatt cgctccgggt
     2761 ggacggcctg tgaagaaaag gatcgtcgag atgcgggtgc gccctgatat tccgctccgc
     2821 ctgcccggac cggtcagaca tgcggggcaa ggtcaagtca tgatcttgtt cgcgttggca
     2881 tcactggtac tgatcgggtt tgtcgcgctg tctatcgata ccgggtttct catgtccgag
     2941 cggcgacaaa cgcagaatgc cgccgacgcc gccgcactgg ctgcggccaa gtcgctgttc
     3001 gatggcaagt ccggccagat ggtggctgcc gccaaggcct acggagccga caacgccgat
     3061 gtgtcccagg acaatgtcac tgtcagttgg ccacccgcca gcggcgagca cgccggcgac
     3121 aaccagtacg ttcaggtcac ggtcaccaag gatgttgaaa agttctttgt cggcgctctc
     3181 tacacgggag attggaaggt cagcgcctca gccgtcgcag gtatcgagcc gcaaggcgcc
     3241 aactacgcgc tcattacact cgataggacc cacaatcctg ggatctacat gaacggcacc
     3301 acgacgatcc ggctgatagg cgatcacgcc agcgcctatg gcagcactga tgtcgacggc
     3361 aacggcaagc tctacgtcga cggcgccgtc gacagccacg gctcggtttc cggcgtcggc
     3421 tcggccccag acggcatcca taccaacatg ccgtatatcc ccgacccact ggacggcgtt
     3481 ccggcgccaa gcaagccggc aaccgtgcga acgggctgca ccggagactg cgatctccag
     3541 cccggttact acaagaacca gacgatcctg tgcaagtggg tctgcaactt cgcacctggt
     3601 ctctattact tcgagaacac gtcggtcaag agccaatcca cgaagtctga gatgaatggc
     3661 cctggaggag tgatgttcta cttcactggc tcaagcgaat tcgactcaaa gaacgggggg
     3721 gtaaacctac gagcgccggc agcatctccg tacgcgggag gacaggaagg tgtcgtattc
     3781 tggtcggcat cgtgcaatga gatcaatttc caaggtaacg gagacttcta catgcacggg
     3841 atcgtttacg ccccctgtgc ggacgtatcg atgaacggaa ctcccggcgg cgacggtgtg
     3901 aagggtcagt ggatcgtcaa tacattcaaa gtcgtcggta caagtgactt cagattgaat
     3961 taccacagtt acgtcgatac gaagcgcccg aagatcttcc tggtcggcta gcggccagct
     4021 tccaaatccc gaactgcgac ggcggccggt acccatcggt cgccgtcgca gcattccccc
     4081 ggcgacacac gacatgcgac aatagcgggt ggcacagtca cgcgaacgac gacgcgtctg
     4141 ggatacaggc agcctgacgg ctgaaaaggg gtgggacatg cagatcacac gctaccggcg
     4201 ggacgggcag gatcgggtag gcgtcatcga gaacaacatc gttcgcgaaa cgcgcggcga
     4261 catgttcggc aagctctcga tcgggccgga gatcgcgccg gtcggcgaga tggagctgct
     4321 gccgccggtt gtccccagca agctgctggc gatcggcctc aactaccagg accacatcag
     4381 ccaggacgca cccggcttcg agacgccgga gaacccgatc gtcttcctca agccgccctc
     4441 cgcgctgatc ggccacggcg ggcagatcgt tctgccaccc gggctggaga agatcgacgc
     4501 cgaagcggaa ctggccgtcg tcatcggccg cgaatgccgc cacgtcaagg ccgcgcgggc
     4561 ctgggatgtc gtgctgggcc tctgcccctc gaacgacgtc agcgcccgcg actatcagtt
     4621 caaggacggc cagtggtcgc gcgctaaggg gttcgacacg ttcgcgccgc tcggcccggc
     4681 gatcgtgacc ggcgtgacgg ccgaagacct cgccatcact tgccggatca acggcgagat
     4741 ccgccagcag tcgtcaacga agcacctcat cttcgacatc ccatacctga tcgagttcct
     4801 gtcgcgggtg atgacgctgc tgcccggcga cgtcatcatg accggcacac cggccggccc
     4861 gccgaagctg gcccccggcg atgtttgcga ggtcgagatc gcccagctcg gcgttctccg
     4921 caacacctgc gtcgccgagc ctgacctgcc ggcatgacga tgcctgccgc cgggctgcac
     4981 ctcagaccgg cgacgctcga tgacgtgccg cgcctgaaag agctcggtgt tctcggctgg
     5041 gagacgacct accacgacta cgtctccccc gagaaccgcg ccgcctacct ggccggtccg
     5101 ttctggtcgc tggagcggct ggcggctgtc gtcgacgacc cggccagcct cacgctggtc
     5161 gccacgaccg gcgcggaacc agccgccggt ttcctgacga tcgaaccggt cgaaaccagc
     5221 gtcgtcgagc tcacgcgcct gtacgtcgat cccgccctgc gtggcctcgg cgccggcgcc
     5281 gcccttttcg acgccgccag gggttggtcc cgcgatcacg gcgcccgaac gatcctcgtc
     5341 aacgtcttcg ccgacaacac cggcggccgc cgcttctacg aacgcgccgg ctgcaccctc
     5401 atccgcctcg agccaaccac cgtcggggac cagaccgtcg cggatgcctg gtacgagtgc
     5461 gaggttttgg gtagtaggtg ataggagttg ggagttgggt cgctgggtgg agcgtggcga
     5521 attcgtctcg cgctgacaag cctcgagcac ctatcaccta ctacccaaga cctcgacacc
     5581 gcccgccgtc ctgcgtacac tccgaaccgt tatttcggtg gggatggatg gtccggacgg
     5641 ggaacgcaac gggattcggg gtgttccctg gtggggttca gcgacatgca gcctgtgacg
     5701 acagggcgta aacacggacg acagctccgc ctgctgctgc tggcgacgct ggtcgtgtcg
     5761 ctggtgacac tgagcctacc ggcaaccgct gttgaggcgc cgtcgccgcc ggaggtcttc
     5821 gccgagaacg cggtcgtcct ctccgacttc aacgaagtcc tctacgacca caacgcccat
     5881 cagcgcacag cgatggcctc gacgaccaag atcatgacgg cgatcctggc cgtccgctac
     5941 ggtaacctcg atcaggacgt gctgattgac gagtccgaca tcgtcggaga ggcgtcgatg
     6001 gggctctatg ccggtgagac cgtcaaactg cgtgacctgc tctacgggct gctgctgccg
     6061 tccggcaacg acgccgccca cgcaatcgcc cggaccgtcg gctggaagcc cggcacgacg
     6121 accgccgatg aagccgtcaa caacttcgtc ggcctgatga accagaccgc cagtgagtac
     6181 cagttgcgcg atacccactt catgaacccg cacgggctcg acgcctgggg ccactactcg
     6241 accccgttcg acctcgccat catgctgcga gccgccctca actacgacgt gatccgcgag
     6301 acgatgcaga cgctctccat caacgccggt ggacacgacc tctggaacca caaccagctg
     6361 tacggcatgc gtgacgacat gatcggcggt aagaccggct acaccgaggc cgccaacttc
     6421 tgcctggccg ccgccgcagg ccgcaacggc cgcttcgtca tcgccgtcgt cacccgtgat
     6481 gacggcgaca actgggtcta cgacgtcagc cagctgctcg actggggtct gatcgctgtc
     6541 gaccgccagg gcatcccccc gggcgtcaat cccaacctga tcggcaccca gcttaaccag
     6601 ccagacccgg tcatccagca gggatcgccc tgacatagcc ggtagtcacc ctttttccgt
     6661 gacgctatac tgaatccatg aacagtgtcg ccaaacggac cccacttctc agcgtcgagg
     6721 actacctgga gcttgaagca tccagctcga tccggcacga gtacattgac ggtcacattt
     6781 atgcccttgc tggcgtcacc aaccgccacg acgacatcgt cctcaacatc atggctcgat
     6841 ttcacgcggc agcgcgcggt accgagtgcc gcgttcgaag cggggaagtg caactcaggg
     6901 tttcggcttc gcgatattac taccccgata ttcacgtcgt ctgtcatccg atttcgtccg
     6961 aggcaagatc tgtaagcgac ccatgcgtca tcgtcgaggt gctttctccg tcaaccgagc
     7021 aggttgacca gcgggagaag ctcttcgcct acacaactat ccagacattg cgcgcctatt
     7081 acatcgtcca ccaggaccgc atgaccgtcg aaaaccactg gtgcgacgac gaaggcacct
     7141 ggtggcacgg cacgctccat ggcggcggcg cgctcaaggt tccctgcctc aacgtcgatc
     7201 taccgctcga acagatctac gagggcgtca ccttcgaaca gcagtagttg cttcagcttg
     7261 ccgatctcac acgcagcgga acggatggtc caacgccaat gagcagcatg gcccaccggc
     7321 gggagcccct caccacagcc gaatacctcg agcttgaatc ccgcagccct gtgcggcacg
     7381 aatttatcga cggcttcctt tacgcgttgg caggcgtctc aaaccgacac gatcgaatcg
     7441 tcgtcaacct catcgtctcg tttggatccc gtgctcgcgg tactgattgc caggtccata
     7501 ccggcgaaat gatggtgcgt gtctcaccga ctcgctacta ctatcctgat gtccatgtca
     7561 cctgccaacc agaaacggac gactcccgaa ccatcactgc accgtgcgtt gtcgtggaag
     7621 tcctctcgcc atcgacgacc ggtactgatc gcagagaaaa gctctgcgcg tatcggcaga
     7681 tcgaaacgct gcgcagctac ctgattgtcc atgcggatga aaaggtgatc gaacagcatt
     7741 ggatcgacaa cggcgtctgg cgacatgaca tcctggaaga caacgcttcc gtccatattc
     7801 catgtctcga cttcgatctg gatcttgccg atatctacga gggcgtgacc ttcgaagaaa
     7861 gctgacgcct cgcgtcgggg cgatggccgc agcctcgccc tgcccagatc gcacgaacaa
     7921 ggg
//
LOCUS       DASUGJ010000692         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1918028, whole genome shotgun sequence.
ACCESSION   DASUGJ010000692 DASUGJ010000000
VERSION     DASUGJ010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918028"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1162)
                     /locus_tag="VFV93_15715"
     CDS             complement(173..1162)
                     /locus_tag="VFV93_15715"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEX5166852.1"
                     /translation="MNASSGRIAVPLLVTPSYDPKTWGGRRLEQLGKVLPGGAIGESL
                     ESGPAAVVNGGPFDGVTLGELSRTHAAALLGSRGQADSGAFADFPLLVKLIDAHHDLS
                     VQVHPDDARAPTGKRGKTEAWYILDAEPGARIITGVSGPIQIERIGEQLVESVVRPGE
                     VYFVPAGTVHAIGAGVLLYEIQQASDVTWRLFDWGRPREIHVEAALAAADPARQAVRI
                     ESCRITEWCEVLVACPYFALERWMVDKSASLPAFPSAFRVVTVLEGELVAGDVRVPHG
                     ATVVLPADLPESELTGRGIALVGYIPDLDVDVRAPLLAAGHDVQAIERLGLRS"
     gene            complement(1159..1725)
                     /locus_tag="VFV93_15720"
     CDS             complement(1159..1725)
                     /locus_tag="VFV93_15720"
                     /inference="COORDINATES: protein motif:HMM:NF037640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5166853.1"
                     /translation="MILAFGRQPDAELALQAQAALAYSPIDGVFVPWRDETLATRLAG
                     VYIAGDAGGIGTVARSYAEGLVAGVAASGGDGLPDALERLRAIASDHVPDSSVPAIAD
                     ATLICRCEEVSAASIRTAINTGSNSLNDIKRRTRAGMGICQGIYCHRTVARMLSAETG
                     IPLDTVVPMTARPPARLLSMAAMADLEP"
     gene            complement(1762..2424)
                     /locus_tag="VFV93_15725"
     CDS             complement(1762..2424)
                     /locus_tag="VFV93_15725"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5166854.1"
                     /translation="MREADAALQAGRLMRETDLASAAGRPMRETDVAVVGAGPAGIGA
                     ALAAARNGASVVLIDENSAVGGHLRWTLAAQTGFDASIDGRSGIEVASWAAGALADAG
                     VEVLTRSVAWGLFEENVLGVATPEASYQLQARAVIVTTGATDIVWPFPGWQLPGVMTA
                     TAALRAMHLDRVLPGLHCVVLGAGLLAEAVASDITSCGADVVLRAGNVNGLVAGGDDA
                     VG"
     gene            complement(2424..>2645)
                     /locus_tag="VFV93_15730"
     CDS             complement(2424..>2645)
                     /locus_tag="VFV93_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5166855.1"
                     /translation="EPIAAALLAHGVVAGRTMPETDAPRGYFCGVGRCPDCSMIVDGE
                     LNVRTCVTPVRDGMAIETQHGLGSWKVGG"
BASE COUNT          475 a          919 c          838 g          413 t
ORIGIN      
        1 gagagcatac cgggtggcat gctcacccgt tcaagattcg gggtgagcgc gtcgatcgcc
       61 gcttgacgcg catcgagatc acgcaggagc gtcaccgctg cgtctctcgt gaagcgatcg
      121 aagagcagct ccagcaacgt cgccatccag cgatagtccg atcgtccggc cgtcatgatc
      181 gaagaccgag ccgctcgatt gcctgaacat cgtgaccggc ggcgagcagc ggcgcgcgga
      241 catcgacatc cagatcgggg atgtagccga cgagtgcaat accccgtccg gtcagttcgc
      301 tctccggaag atctgcgggc agaacgacgg ttgctccgtg tggcacgcga acgtcgccgg
      361 cgaccagttc cccctccaga acggttacga ctctgaacgc cgatgggaag gccggcaacg
      421 acgcgctctt atcgaccatc cagcgttcaa gcgcgaagta cggacaggcg accagtacct
      481 cgcaccactc agtgatgcga cacgattcga tacgcaccgc ctgtcgggca ggatcggcgg
      541 ccgcgagcgc ggcctcgacg tggatctcgc gcggccgtcc ccagtcgaac agccgccagg
      601 tgacgtcgct ggcctgctga atctcgtaga gcagcacccc ggcgccaatc gcgtgaaccg
      661 ttccagccgg cacgaaatag acctcgccgg gacggaccac actctcaacg agttgctcgc
      721 cgatccgctc gatctggatc ggcccgctaa cgccggtgat gatccgagcg cccggctcgg
      781 cgtcgaggat gtaccatgct tcggtcttgc cccgcttgcc ggtcggggcg cgggcgtcgt
      841 ccgggtgaac ctggacggag aggtcgtggt gggcgtcgat cagcttgacc agcagcggga
      901 agtcggcaaa cgcaccggag tccgcctggc cacggctgcc gagcagtgcg gcggcatgtg
      961 tccgggacag ctcgccgaga gtcacgccat cgaacggtcc gccgttcacg acggcagccg
     1021 ggccactttc gagcgattca ccgatcgcgc cacccggcag gaccttgccg agttgctcca
     1081 gccgccgccc accccaggtt ttcgggtcgt agctgggcgt tacgagcagc ggtacggcga
     1141 tcctgccgga actcgcgttc atggttccag atcggccatt gccgccatcg agagcagccg
     1201 ggccggggga cgtgcagtca tcggcacaac cgtgtccagc ggaatcccgg tctccgccga
     1261 gagcattctg gcgacggtcc ggtggcaata gatcccctgg cagatgccca tcccggcacg
     1321 cgtccggcgc ttgatgtcgt tcaggctgtt tgatccggtg ttgatcgctg tccggatcga
     1381 tgcggcgctg acttcctcgc agcggcaaat cagcgttgca tcggcgatgg ccggcaccga
     1441 cgaatccggc acatggtcgg atgcgatcgc gcgaagccgt tcgagcgcgt caggtagacc
     1501 atcgcctcct gaggcagcca cacccgcaac cagcccttcg gcatacgacc gagccacggt
     1561 acctattccg ccagcatcac cggcaatgta gacacctgcg agccgggtgg caagcgtctc
     1621 gtcacgccac ggaacgaaga cgccgtcgat cggcgagtat gcaagcgctg cctgcgcctg
     1681 gagcgcgagt tccgcgtccg gctggcggcc gaaggcaaga atcaccgcgt cgcaggcagt
     1741 gcgtagcccg tccgactcga cctatccgac ggcatcgtcc ccaccggcaa ctagaccgtt
     1801 gacgttgccg gctcgcagga cgacgtctgc gccacaactc gtgatatcgg acgcaacagc
     1861 ttcagccagc aacccagcgc cgagcacgac gcagtgcaag cccggcagca cccgatctag
     1921 gtgcatcgct cggagtgctg ccgtcgcggt catcacgcca ggcagttgcc agccggggaa
     1981 cggccagaca atgtcggtcg caccggtcgt gacgatcacg gcgcgggcct gtaactggta
     2041 cgacgcctcc ggagtcgcga cgccgaggac gttctcctcg aagagccccc aggcaaccga
     2101 gcgcgtcagc acctcgacgc cggcgtccgc aagcgctcct gcggcccaac tggccacctc
     2161 gatgcccgac cggccatcaa tcgatgcatc gaatccggtc tgggccgcca aggtccagcg
     2221 cagatggcca ccaacggcgc tgttctcgtc gatcagaacg acactggcgc cgttgcgggc
     2281 ggccgccagt gccgcgccga ttccggccgg accggctccg acgacggcga cgtctgtttc
     2341 gcgcatcggt cgcccagctg ccgaggcaag gtctgtctca cgcataagcc gaccagcctg
     2401 gagggcggca tcagcctcgc gcatcagccg cccaccttcc agctaccgag gccgtgctgg
     2461 gtctcgatcg ccattccgtc gcgtactggc gtgacgcagg ttcggacatt tagctcaccg
     2521 tcgacgatca tcgagcagtc cgggcaacgg ccaacgccac agaaatagcc gcgcggcgcg
     2581 tccgtctcag gcatcgttcg gcctgccacg acgccgtgtg ccagcagcgc cgccgcaatc
     2641 ggttc
//
LOCUS       DASUGJ010000693        13032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1500812, whole genome shotgun sequence.
ACCESSION   DASUGJ010000693 DASUGJ010000000
VERSION     DASUGJ010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13032
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500812"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1216
                     /locus_tag="VFV93_15735"
     CDS             266..1216
                     /locus_tag="VFV93_15735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5166856.1"
                     /translation="MISVTANVKRGTHRVIAHAVARLLRAGAVYDPVNFRIWEQRGYH
                     VTPIHFYYPIPDTSELRRRYPTGMAGAGYDLQWDAQREFLRQVIAPYADECADLYRRP
                     PDDQRFYLDNDAFSGIDPYVYYAMIRHLKPESVLEVGSGFSTMLAAEAVERNGTGTVK
                     CIDPWPRAFIKNGSIPRIPQRVEDVDPAVFQTLQAGDILFVDSSHVVRTGGDVCHIVL
                     NVLPVLQPGVIVHFHDIYLPFEYPPDLVLDRLQFWTEQYLLQAYITENPSVSVLFACH
                     AMLREHPNDMREYLPLATPFGASFWIQKMETDASSAGKSS"
     gene            complement(1287..1778)
                     /locus_tag="VFV93_15740"
     CDS             complement(1287..1778)
                     /locus_tag="VFV93_15740"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5166857.1"
                     /translation="MRRWLPVLMALMLVMAMTVACGDDDDSDDDGGAAPTATTATTAT
                     QAATTPSGGAATATTGDEDDGDDEGDAAAGQQIAQAQCFTCHTIDGSTLVGPSWKGLY
                     DHDVKLDDGSTVKADEDYIRESILEPNAKIVEGFPAAMPSFDGILDDTQIANIIAYIK
                     TLE"
     gene            complement(1874..2344)
                     /locus_tag="VFV93_15745"
     CDS             complement(1874..2344)
                     /locus_tag="VFV93_15745"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEX5166858.1"
                     /translation="MIARGFLLLAMLLAALTMTACGDEAKASDPPDLTVGESTCSRCH
                     MIISDERFASGLTFEDGDPLLYDDLGEMITVIQTEDPHADYIWVHDYDSKEWIDATMA
                     WYVDSAEIMAPMGSGLAVFETREAAEAFAATNDGTVRDWPAILADWRMPMRMQH"
     gene            complement(2341..3171)
                     /locus_tag="VFV93_15750"
     CDS             complement(2341..3171)
                     /locus_tag="VFV93_15750"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166859.1"
                     /translation="MNWSAVATIAGRDARAGLRNRWFLLYSALFLVMSVGFSWVALAG
                     SDVTGQAGFGRTSAGLLNLMLLMVPLIGLTVGAQSIVGDRQDRSLDYLLAQPVSALEV
                     YAGKYLGAAFSIVLLLVFGFGWSGIVMAVRGSASSASDFILLVVLTTLLALGMLSVGF
                     LISSVSPQTAAALGIAVSLWLGFVVIGDLGIMGSALVMNLDPSALLGLTLANPLDTYK
                     LLSVDLLGASLDVLGPAGVYATDRFGGNLAAYLLGVEALWVLLPLPLGYWLFQRMDVR
                     "
     gene            complement(3168..5540)
                     /gene="nosD"
                     /locus_tag="VFV93_15755"
     CDS             complement(3168..5540)
                     /gene="nosD"
                     /locus_tag="VFV93_15755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HEX5166860.1"
                     /translation="MSPRANLLLIAFVLLALLPLGEFRAAGAQETQVDIVVCPTCEVS
                     DLAEAVSNAAPGSRIEVRGGTYATQLLIERDLELIGVDNPIIDGGGQGTVIRVTGAAL
                     TIEGFMIRNSGTSLDHEDSAVLVEKGQATVIDNLIEGTLFGIYLKESPGSIVRDNVII
                     GEPLPPALRGDGIRVWYCDDVLIEGNVAEDGRDAILWYSNRGVVRNNRFDRGRYGLHL
                     MFSDAARIEGNSLNENSIGLYIMYSRDPHIVGNSMSNNRGPSGGGLGLKDVDRAIVEA
                     NRFVGNQIAAQVDTSPREFGIENYFTDNVFAYNEVGIGFMPSVRRNTLSGNSFIDNIV
                     HVSVLGGGKLQEITWSPDGRGNYWSDYAGYDANGDGVGDVPYRSQKLFESLMDAHPSF
                     RLFIFSPAAMAIDFAARAVPSVRPETRFEDTAPLMEPPAVAGLPPVEQAGMATRLGWA
                     GAGLAVVLGAATAASAIRRRPARHTIQQTEEATVRGMQTPHAPAAVTGPEGESAAIVA
                     RGLTKRYGKVAAVDDMTFAIRPGDAVALWGPNGAGKTTILRCLLGLARYSGHVMVEGL
                     DPARQGRETRQAIGYVPQDLAPSAMTVAEMTAFIAKLKGVALDDARERLVLLGIEDAA
                     DKPVSALSGGMKQRLALALALIGAPSILLLDEPTANLDAAGRADLLNLLRRLKADGMT
                     LVFSSHRPDDVLTLADRILLIERGVLQEELTPAAFATSLDAGSRLVVALKNGHMNEAL
                     ETLTRLGYEPVRGGDRIVTVGIHPTQKAEVLMALAREGVDIADFELERMS"
     gene            complement(5524..6186)
                     /locus_tag="VFV93_15760"
     CDS             complement(5524..6186)
                     /locus_tag="VFV93_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX5166861.1"
                     /translation="MVTVDRAPAMPATAQKAASQRARPDMVGLVRRSPLRFAALVVAA
                     GLLVASILLPYWSITLHAPQYPGGLTVDAYTYKLTGDVSEVDGLNHYIGMMQLGEAAK
                     LERAISRIAIPVIALLAVASFFVVSRWRWLLAAPVIAYPIIFMVDLFIWLYYAGHSLD
                     PNAALSSSIREFTPRILGKGVIGQFSTQATLDTGFYFILVAAVLVIVATYRKRANDVT
                     AS"
     gene            complement(6259..8304)
                     /gene="nosZ"
                     /locus_tag="VFV93_15765"
     CDS             complement(6259..8304)
                     /gene="nosZ"
                     /locus_tag="VFV93_15765"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HEX5166862.1"
                     /translation="MADALARARFNRRTLLKGAAGAAVLGPAGGVLLASCMGGDDDDN
                     GNSGGSAGSLPASAQEVAEARGLAPEDITRALKTYMPGGQYDEFFIMASGGHSGQVLV
                     IGVPSMRLLKVIGVFTPEPWQGYGFSDDTKKILEEGAVGDVVHTWADTHHPGLSETNG
                     EYDGEFLFINDKANARMAVIDLRDFETKQIVKNPHIISNHGGAFVTPNTEYILDGSQY
                     AAPYGWEYAPLSEYKEKYRGAMTFWKFDRDKGRIDPEQSFSIELPPYWQDLADSGKGP
                     SGDWMFSNSFNVEMSIGGNRADGSKMVPPVEAGASQRDMDYLTMINWRKAEELFKSGA
                     GEDIHGMQLIRIPDAVEAGILFQIPEPKSPHGVDVTPDGKFIVVAGKLDPHVTVYSFE
                     KIQAAIAAGDVEMDEFGVPILPYEACMEAQVELGLGPLHTQYDDKGYAYTSLFLDSAI
                     ARWKIGEAGKPETWTMIDKIQVHYNVGHLSVCEGDTVAPKGKYLVALNKWAIDRFANV
                     GPLHPQNFQLIDISGETMDLLYDMPIGIGEPHYVQTISADKIKAWPIYPEVGWDPIAQ
                     APSEVATQAGSESVERDGNVVTINMTSIRSHFTPDTVEVHEGNEITWHITNIEKAEDA
                     THGFALPGHNINLSIEPGETTTMTFTADKPGVYPFYCTEFCSALHLEMMGYFIVEPK"
     gene            complement(8571..10523)
                     /locus_tag="VFV93_15770"
     CDS             complement(8571..10523)
                     /locus_tag="VFV93_15770"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5166863.1"
                     /translation="MEAGRTSRLLSRLDPARFEASGERATLIAVGMTLLALAGTVIVD
                     IWVIDPRLAGFYVAVAIAAWYGGWRGGLLAAVLSGLVSSWAFLDPKYTLALKLPSSLV
                     SIVAMLLVGLVMSALSEASRQAIRRAEAAEQRVRILADASALLVSSLDYERTLAHVTE
                     LAVPQFADWCTVNIVEEDGSIARVALTHRDPAGAAALAEIQRRYPVQPDSQHPLALAI
                     RSGEPGLYPVITEHSQHAWTENDEHRRLVLQLGTTSVLVVPMQARGRALGAVIYARTG
                     GPAYTEDDLRTAGVLTRRAALAIDNARLFRTAQAAEARYRSLFEGSQDAIIVIGADRQ
                     YLAISPAIEKLLGYTPQEMLQMRAGDLLHRRDLWLANAQPLLEQEGTWRGEVELRHKD
                     GHLVPIESRMSRIELPDGPVTFSVWRDISERRAVERLQQEFIVMVTHDLKTPLTSIKG
                     LAQLMLRRQAFSGPSLETIVGQSNHLERLINDLLDVARMEAGRLDLQLAPVDLGQLAQ
                     LAADQAQATTSRHQIRVEIAEQPMIHDWDGDRVRQVLLNLLTNATKYSPAGGEVVIRV
                     EDDPRRQQATVSVTDNGVGIAPDVLPRLFSRFYRAEASLTAGEGLGLGLYISRSLVVA
                     HGGRIWVDSTPGIGSTFTFTLPYSPS"
     gene            complement(10645..10896)
                     /locus_tag="VFV93_15775"
     CDS             complement(10645..10896)
                     /locus_tag="VFV93_15775"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX5166864.1"
                     /translation="MADATIDTIRLDVREIAPRERHPMIFNGFDSLKPGQTLHLVNDH
                     DPKPLYYQLMAERTGQAGWEYVESGPEVWQVRITKLALA"
     gene            complement(10896..12233)
                     /locus_tag="VFV93_15780"
     CDS             complement(10896..12233)
                     /locus_tag="VFV93_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166865.1"
                     /translation="MSAGISSAHAPALDLPARFMALGMLGLAVVAAVSPWAYSLLLDG
                     FYAPRLLAFVHLNTLGVIAAVILGASYQLVPVVLQTELASVRLGRVSFWLYLSGLVLF
                     LPGLLTTWRPALGLGATLVFSGLIVYLVVIAWTLKRAPRMDVVAWHIVAALAGLGGGV
                     ALGLLLALNKGTGFLGALTLRLLAAHATLMLGGWVAVMLSGVAYRLVGMFTLSEDRLW
                     HRVAWVALALSGLGPWTLAIGLIAEAPRMILLAGALLLAGGQLLFGVQLVHLYQVRRR
                     RGVDVHIPFALVSAGCGVGTGALLVWGIASGVGPDSLLWVAAGWLAIAGLTLTAIQGF
                     FYKIATFLVWLHRYAPLAGRQRVPRLEQLYSSRLARAGWALWSLALAGSMLALALDDR
                     LLSRLAGLAATLGLACFLGNVARVGAHWQLASAGRPRLISRLRKPGTAPKGHA"
     gene            complement(12233..>13032)
                     /locus_tag="VFV93_15785"
     CDS             complement(12233..>13032)
                     /locus_tag="VFV93_15785"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX5166866.1"
                     /translation="VLQRYPDLLDVFIATDPAFSKLRNPVLRRVQSRLVTVEQAARIA
                     GLEPPELVRTLNQHLGLEPDAEETVANPADAPVASPPPWVGAAPVAVELDVREHQRRG
                     EEPFGPIMEAVRQVPPGQVLVLRNTFDPIPLYDVLGMRGFEHWAERHAADDWEISFYN
                     TGRSRQRQPEPVATHADDQPLDWDAAFATITIDVSELVPPEPMIRILEALEDLPAGGS
                     LHVHHLRRPMHLYPRLDELGYRHETRELTPGHVEILIQKPAAGDGGS"
BASE COUNT         2360 a         4246 c         4181 g         2245 t
ORIGIN      
        1 accgtcgcaa ctgagggcac gcaaccatcc gaagcattgc tataagccag gaacgtgaat
       61 tcgcgtgtcg ttcgctgatc caagcaaccg cgggctcacc tacgatggct gaatgacgac
      121 tcagtcattg actgctcaaa agatgaacaa attgttcggg ttcatggtac agctgaccgt
      181 ggagacactg gatgcctgct ggtatgaata caccatctag tcaggaagag gaggttgtgc
      241 gtttggctta ctgggaggat tagcaatgat atccgtaacc gccaacgtaa agcgtggcac
      301 gcatcgagtc atcgctcatg cggtcgcccg gctattacgt gctggtgccg tctacgatcc
      361 cgtgaacttt cgaatttggg agcaacgtgg gtaccacgtc acaccgattc acttctatta
      421 cccaatcccg gacaccagcg aactgcgtcg acgatacccg actggcatgg ctggagccgg
      481 atatgacctg caatgggacg cgcaacggga attcctgcgc caggtaattg cgccgtacgc
      541 agatgagtgc gctgacctct atcggcggcc accagatgat cagcggttct atttagacaa
      601 tgacgccttt agcggcatag acccgtatgt gtactatgca atgatccgtc atctcaagcc
      661 cgagtccgtg ctcgaagttg ggtcagggtt ctcgacaatg ctggcagcgg aagctgtcga
      721 acggaatgga acagggacag tcaagtgcat tgatccctgg ccaagagcgt tcatcaagaa
      781 tggttcaata cctcgcatcc cgcaacgggt tgaggatgtc gatcctgcgg tattccaaac
      841 gctacaggcc ggtgacattc tcttcgttga tagttcgcac gtcgtccgga caggtggcga
      901 tgtctgccat attgttctca acgtcctccc agtgctgcag cccggagtca tcgttcactt
      961 ccatgacatc tacctcccgt tcgagtatcc tcctgacctg gtccttgatc gtcttcaatt
     1021 ctggacagag cagtatcttc tccaggcgta tatcaccgag aatccgtccg tatcggtgtt
     1081 gtttgcgtgt catgcgatgc ttcgcgagca tcccaacgac atgcgcgaat accttccatt
     1141 ggcgacgccg tttggagcaa gtttctggat ccagaagatg gagaccgacg catccagcgc
     1201 aggcaaatcg tcgtagtgga ttgctcaaca aacgacggga gcggggtggc gaccccgctc
     1261 ccgtcccaac cccagcgtcc tggcggctac tccaacgtct tgatgtaggc gatgatattg
     1321 gcgatctgcg tgtcgtcgag aatgccgtcg aagctcggca ttgcggccgg gaagccctcg
     1381 acgatcttgg cgttcggctc aaggatcgac tcgcggatgt aatcctcgtc agccttgacc
     1441 gtactgccgt cgtcgagctt cacgtcatga tcgtagagcc ccttccagct cggtccaacc
     1501 aacgtgctgc cgtcgatcgt gtggcaggtg aagcactgcg cctgggcgat ctgctggccg
     1561 gccgcggcat cgccctcgtc atcgccgtcg tcttcgtcgc cggttgtagc cgttgcggct
     1621 cctccggacg gggtcgttgc ggcctgggtc gcggttgtcg cggtcgtcgc ggttggagca
     1681 gcgccgccat cgtcgtcact gtcatcgtcg tcaccgcatg cgaccgtcat cgccatgaca
     1741 agcatcaggg ccatcaggac aggcagccat cgtctcattc ggagccactc cttcattcgt
     1801 gccactccag catggagcca tcggtgtcat tgtgccgctg atcgggccgc aaggccagtg
     1861 ccgcgatgag aattcagtgc tgcatcctca tcggcatccg ccaatcggcg aggatcgccg
     1921 gccagtcgcg aacggttcca tcgttggtcg cggcgaaggc ttcggctgct tctctcgtct
     1981 cgaagacagc caggccagag cccatcggcg ccatgatctc ggcgctatcg acgtaccagg
     2041 ccatggtcgc gtcgatccac tccttcgagt cgtagtcgtg gacccagatg tagtcggcat
     2101 gcgggtcttc ggtctgaatc accgtgatca tctcgccaag gtcgtcgtac aggagcggat
     2161 cgccgtcctc gaaggtcagc ccgctcgcga agcgctcgtc ggaaatgatc atatgacacc
     2221 gggagcaggt cgattcaccg accgtcaggt cgggtgggtc agaggctttc gcctcatcgc
     2281 cgcaggccgt catcgtcagc gccgccagca gcatcgccag cagcaggaat ccacgcgcga
     2341 tcatcgaaca tccatccgtt ggaagagcca gtagccgagc ggcagcggca gcagcaccca
     2401 gagtgcctcg acccccagca ggtaggccgc caggttgccg ccgaaacgat cggtggcata
     2461 gacgccggct ggaccgagga catcgagcga ggcgccgagc aggtcgacgc tcagcagctt
     2521 gtaggtatcg agcgggttgg ccagtgtcag acccaacagc gcagaaggat cgaggttcat
     2581 caccagcgca ctacccatga tgccgagatc gccgatcacg acaaagccca gccagagcga
     2641 gacggcgatg ccgagcgccg ctgccgtctg cggcgagaca ctggagatca ggaagccgac
     2701 cgagagcatc cccaacgcca gcagcgtcgt caggacgacc agcaggatga agtcagaagc
     2761 gctgctggcg ctgccgcgaa cggccatcac gatacccgac cagccaaatc cgaagaccag
     2821 cagcaggaca atcgagaagg cagcgccgag atacttgccg gcgtagacct cgagcgccga
     2881 aaccggctga gccagcaggt aatccaggct gcggtcctgc cggtcaccga cgatcgactg
     2941 ggcgccaacg gtcagcccga tcagcggaac catcagcagc atcaggttga gcagaccagc
     3001 cgacgtacgg ccgaatccgg cctgaccggt gacatccgaa ccggccagcg cgacccagga
     3061 gaagccgacg ctcatgacga ggaagagcgc cgagtagagc aggaaccagc gattccgcag
     3121 gccagcccgg gcgtcacggc cggcgatggt ggcgacagca ctccagttca tgacatgcgc
     3181 tccagttcaa agtcagcgat atcgacaccc tcgcgggcca gtgccatcag gacttcagcc
     3241 ttctgcgtcg gatggatacc aaccgtcacg atccgatcac cgccccgtac cggttcgtag
     3301 ccgagccgcg tcagcgtctc gagcgcttcg ttcatgtgcc cattcttgag cgcgacgacc
     3361 agccgtgagc cggcgtccag gctggtggcg aaggcggctg gagtgagttc ctcctgaagg
     3421 acaccccgct cgatcagtag tatccgatcg gccagcgtca ggacatcgtc aggccggtgc
     3481 gacgagaaga cgagcgtcat cccgtccgcc ttcaaccggc gcagcaggtt gagcaggtcg
     3541 gcccggccgg cggcatcgag gttggcggtc ggctcgtcga gtagcaggat cgagggcgcc
     3601 ccgatcagtg ccaacgccag cgccagccgc tgcttcattc cacctgaaag tgcgctgacc
     3661 ggcttatcag cggcgtcctc gatccccagc aacaccagcc gctcacgggc gtcgtccagc
     3721 gcgacgcctt tcagcttggc gatgaaggcc gtcatctcgg caacggtcat cgccgatggc
     3781 gcgaggtcct gcggcacgta gccgatcgcc tggcgggtct cacggccttg acgggccgga
     3841 tccagccctt caaccatcac gtggccgctg taacgggcca gcccgagcag gcaacgcagg
     3901 atcgtcgtct tcccagcccc gttcggtccc cagagcgcca cggcgtcacc gggccggatg
     3961 gcgaaggtca tgtcgtcgac ggccgcgacc ttgccgtagc gcttggtcag tccgcgcgcc
     4021 acaatcgcag cggattcgcc ctccggccct gtcaccgcag ctggcgcgtg cggcgtctgc
     4081 attcccctca cggtagcctc ctcggtctgc tggatggtgt gccgggcagg acgccggcgg
     4141 atagcgctgg cggccgttgc ggccccgagc acgaccgcca gaccggcgcc ggcccagccg
     4201 agccgggttg ccattcctgc ctgctcgacg ggcggcagtc cggcgacagc cggtggctcc
     4261 atcaacggag cggtgtcctc gaagcgcgtc tcgggccgga cagagggcac ggcgcgggca
     4321 gcaaagtcga tcgccatcgc ggccgggctg aagatgaaca gccggaacga cgggtgcgcg
     4381 tccatcagcg attcgaacag cttctgcgaa cgatacggca catcgccgac cccatcgccg
     4441 ttcgcgtcgt agccggcgta gtcgctccag tagttgccgc gtccatcggg cgaccaggtg
     4501 atctcctgca gcttgccgcc gccgaggacc gagacatgga cgatgttgtc gataaagctg
     4561 ttgccgctca gcgtgttgcg ccggacggac ggcatgaagc cgattccgac ctcgttgtag
     4621 gcgaagacgt tgtcggtgaa atagttctcg atcccgaact cacgcggcga ggtatcgacc
     4681 tgagctgcga tctgattccc gacaaagcgg ttcgcctcga cgatcgcacg atcgacatcc
     4741 ttcagtccaa gcccgccgcc gctgggccca cggttgttgg acatcgagtt gccaacgatg
     4801 tgcggatcgc ggctgtacat gatgtacagc ccgattgagt tctcgttgag cgagttgcct
     4861 tcgatccgcg ccgcatcgga gaacatcagg tggaggccgt agcgtccacg gtcgaagcga
     4921 ttgttacgga cgacgccccg gttcgagtac cagaggatcg cgtcgcgccc gtcctcggca
     4981 acgtttcctt cgatcaggac gtcatcgcag taccagacgc ggatgccatc gccacgcagc
     5041 gcgggcggta acggctcgcc gatgatgacg ttgtcacgaa cgatactgcc gggcgattcc
     5101 ttgagataga tgccgaacag cgttccctcg atcaggttgt cgatgaccgt cgcctgaccc
     5161 ttctcgacga ggactgccga gtcctcgtga tcgaggctcg tccccgagtt gcggatcatg
     5221 aacccctcga tcgtcaatgc cgcgccagta acgcggatga cggttccttg cccgccgcca
     5281 tcgattatcg ggttgtcgac tccgatcagt tcgagatcgc gctcgatcag gagctgcgtg
     5341 gcatacgtcc cgccgcgcac ctcgatccgg gaacctgggg cggcgttcga gaccgcctca
     5401 gccagatcgg acacctcgca cgtagggcag acgacgatgt cgacctgcgt ctcttgcgcg
     5461 cctgccgccc ggaactcgcc gagcggcagc aacgcaagca gcacgaacgc tatcagcagg
     5521 aggttagctc gcggtgacat cgttcgctcg cttccgatag gtcgcaacga tgacaagcac
     5581 tgccgccacg aggatgaagt agaagccggt gtcaagggtc gcctgagttg agaattggcc
     5641 gatgacgccc ttgccgagga tgcgcggcgt gaactcgcgg atcgacgacg agagtgcagc
     5701 attgggatcg agggagtggc ctgcgtagta gagccagatg aacaggtcga ccatgaagat
     5761 gatcgggtag gcaatgaccg gcgcggccag cagccagcgc cagcggctca cgacgaagaa
     5821 ggacgcaacc gccagcagcg cgatgaccgg gatcgcgatt cgcgagatgg cccgctccag
     5881 cttggccgcc tcgccaagct gcatcatccc aatgtagtgg ttcaggccat ccacctcgga
     5941 gacatcgccg gtcaacttgt aggtataggc atcgaccgtt agcccacctg gatattgcgg
     6001 cgcgtggagt gtgatcgacc agtagggcag caggattgaa gcgaccagca gaccggcagc
     6061 tacgacgagc gccgcgaagc ggagcgggct gcgccggacg agccccacca tgtcgggtct
     6121 ggcacgctgg gaggcggcct tctgggctgt tgcgggcatt gctggcgccc gatcgacggt
     6181 gaccatggtg ggtgtctcct ctcgtggcgg gttggccggc ggtcaccgct cgcggatgac
     6241 cgccggccgg atccgctgct acttcggctc gacgatgaag tagcccatca tctccaggtg
     6301 cagcgccgaa cagaactcgg tgcagtagaa cgggtagacg ccgggcttgt cggctgtgaa
     6361 ggtcatcgtc gtcgtctcac ccggttcgat actcaggttg atgttgtgcc ctggcagcgc
     6421 aaacccgtgg gtggcgtcct ccgccttctc gatgttggtg atgtgccagg tgatctcgtt
     6481 accctcatgc acctcgaccg tatccggcgt gaagtggctg cggatcgacg tcatgttgat
     6541 cgtgaccacg ttgccgtccc gctcgacgct ctcgctgccg gcctgggtcg caacctcgga
     6601 cggcgcctgg gcaatcgggt cccagcccac ctcgggatag atcggccagg ccttgatctt
     6661 gtcggcgctg atcgtctgga cgtaatgcgg ctcgccgatg ccgatcggca tatcgtagag
     6721 caagtccatc gtctcgccgg agatgtcgat caactggaag ttctgcgggt ggagcgggcc
     6781 gacgttggcg aagcggtcga tcgcccactt gttgagggcg accaggtact tgcccttcgg
     6841 tgcgaccgtg tcgccctcgc agacgctcag gtgaccgacg ttgtaatgga cctgaatctt
     6901 gtcgatcatc gtccaggtct cgggcttgcc cgcctcgccg atcttccagc gcgcgatggc
     6961 gctgtcaagg aagagcgagg tgtaggcgta gcccttgtca tcgtactgcg tatggagcgg
     7021 gccaaggccg agttcgacct gggcttccat gcaggcctca tagggcagga tcgggacgcc
     7081 gaactcgtcc atctcgacat cgccggccgc gatcgccgcc tggatcttct cgaagctgta
     7141 gaccgtcaca tgcgggtcga gcttgccggc cacaacgatg aacttgccgt ccggcgtgac
     7201 atcgacaccg tgcgggctct tcggctccgg gatctggaac aggatgcctg cctcaaccgc
     7261 gtcggggatc cggatcaact gcatgccgtg gatgtcctcg ccggcgccgg acttgaagag
     7321 ttcctcggcc ttgcgccagt tgatcatcgt caggtagtcc atgtcacgct gcgacgcacc
     7381 ggcctcgacc ggcgggacca tcttcgagcc gtcggcgcgg ttaccgccga tcgacatctc
     7441 gacgttgaac gagttggaga acatccagtc cccggacggc cccttgccgg agtcggccag
     7501 gtcctgccag tacggcggca gctcgatcga gaacgactgc tccggatcga tgcggccctt
     7561 gtcgcggtcg aacttccaga aggtcatcgc gccgcggtac ttctccttgt attccgagag
     7621 cggtgcgtac tcccagccat acggcgccgc gtactgcgag ccgtcgagga tgtactcagt
     7681 gttcggcgtc acgaaggcgc cgccgtggtt ggagatgatg tgcgggttct tgacgatctg
     7741 cttggtttcg aagtcgcgta ggtcgatgac ggccatccgg gcattggcct tgtcgttgat
     7801 gaacaggaac tcgccgtcgt attcgccgtt cgtttccgac aggccggggt ggtgcgtgtc
     7861 ggcccaggtg tgaacgacat cgccgacggc gccctcttcg aggatcttct tcgtgtcgtc
     7921 tgagaagccg tatccctgcc acggctcagg tgtgaagacg ccgatcacct tcagcaggcg
     7981 catcgacggt acgccgatca ccagcacctg gcccgagtgg ccgcccgagg ccatgatgaa
     8041 aaactcatcg tactggccgc ctggcatgta ggtcttcagt gcgcgggtga tgtcctcagg
     8101 cgcgaggcca cgggcctcgg caacttcctg ggccgaggcc ggaagcgatc cggcagaacc
     8161 gccactgttg ccgttgtcgt catcgtcacc gcccatacac gaggcgagca agacgccgcc
     8221 ggccgggccg aggaccgccg cgccggccgc ccccttgagg agtgtgcgcc gattgaaccg
     8281 ggcgcgagcc aatgcatcag ccatcgatct gcccccttct cgggtggtgg cgcgttgtgc
     8341 gccgccaatt ttccgctcct ggatggtggt cggggctagc ctcagcagcg ttcgtcgtcc
     8401 accagtggag cgtcatccat cgcctgccct gagtccaaag cagtgcgagc ctgtcgttgg
     8461 cctcaacgat agttggacag ccgttccggc ctcgtgtcta aattcacggg tgcgttacag
     8521 ttccgtgaca gtcaggtctg gcgacccgga gagcgtcgtt gaagtcgtga ctagctgggg
     8581 gaatacggca gcgtgaaggt aaacgtgctg ccgatgccgg gtgtcgaatc gacccagatc
     8641 cggccaccat gcgcgacaac cagcgagcgc gagatataca gacccagccc gagcccctcg
     8701 ccggctgtca gcgacgcttc ggcgcgatag aagcgactga agagcctcgg caggacgtcc
     8761 ggggcgatgc cgactccgtt gtcggtgaca ctgacggttg cctgttgccg gcgggggtcg
     8821 tcctcaacgc ggatgacgac ctcgccgccg gccggcgagt acttggtcgc attcgtcagc
     8881 aggttgagca gcacttggcg aactcggtcg ccgtcccagt cgtggatcat cggctgctcg
     8941 gcgatctcga cccggatctg atgccggctg gtggtcgcct gcgcctggtc agcagccaat
     9001 tgggcaagct gaccgagatc gaccggcgcg agttgcaggt cgagccggcc ggcctccatc
     9061 cgcgcgacat ccagcagatc gttgatcagc cgctccaggt ggttcgactg accgacgatc
     9121 gtctccagcg aggggccgct gaatgcctgc cgcctgagca tcagttgcgc caaccctttg
     9181 atcgaggtca gcggcgtctt gagatcgtgc gtcaccatca cgatgaactc ctgctggagc
     9241 cgctcaacgg cccgccgctc ggagatatcg cgccagactg agaaggtgac cgggccgtcg
     9301 ggtagctcga tccgcgacat ccgcgattcg atcggcacca ggtgaccgtc cttgtggcgc
     9361 aactcgacct cgccgcgcca ggtcccttcc tgctcgagca gcggctgggc attcgccagc
     9421 cagaggtcac ggcgatggag cagatcgccg gcccgcatct ggagcatctc ctgaggggtg
     9481 tagcccagca gcttctcgat cgcggggcta atagcgagat actgacggtc ggcgccgatg
     9541 acgatgatcg cgtcctgcga gccttcgaag aggctgcgat agcgcgcctc ggccgcctgg
     9601 gccgtccgga acagccgggc attgtcgatc gccagcgccg ctcgccgagt caggacgccg
     9661 gctgtccgca gatcgtcctc cgtgtaggcc ggcccgcccg tacgcgcgta gatcaccgcc
     9721 cccagcgccc gcccacgcgc ctgcatcggc acgaccagca ccgatgtcgt gccgagttgc
     9781 agtaccagcc ggcggtgctc gtcgttctcg gtccaggcat gctgggagtg ctcggtgatg
     9841 accgggtaga gccccggctc gccgctgcgg atggccagcg cgagcgggtg ctggctgtcg
     9901 ggctgcaccg gatagcggcg ctggatctcg gccagcgccg ccgcgccggc cggatcgcgg
     9961 tgcgtcagcg caacccgagc gatgctaccg tcctcctcga cgatgttgac ggtgcaccag
    10021 tcggcaaact gcgggacggc caactcggtg acgtgcgcca gcgtccgttc gtagtccagc
    10081 gaggaaacca gtagcgcgct cgcgtcggcc agaatcctga cgcgctgctc ggccgcctcc
    10141 gcccgccgga tcgcctggcg cgatgcctcc gagagggcac tcatgaccag cccgaccagc
    10201 agcatcgcca cgatcgagac gagcgacgag ggaagcttga gagcaagtgt gtatttcggg
    10261 tcgagaaagg cccagctgct gacgagtccc gagagaacgg cggccagcag cccgccgcgc
    10321 cagccaccgt accaggccgc gattgcgacc gcgacataga acccggccag ccggggatcg
    10381 ataacccaga tatcgacgat aaccgttccg gccagcgcca gcagcgtcat cccgacggcg
    10441 atcagcgtag cgcgctcacc actggcctcg aaccttgccg gatcgagacg gctgagcagt
    10501 cgggatgttc tgccagcctc catgtccgcc caatcccaac acgcgcccgc gccgccggtc
    10561 cggaaggttg tcactgcctc acatacagta tcacctaccg atacgcaggc gagttgagat
    10621 tgggcggcgg atcaagccag atgttcaggc gagagcgagc ttggtgatgc gcacctgcca
    10681 gacctcgggg ccgctctcga cgtactccca gccggcttgg ccagttcgct cggccatcaa
    10741 ctggtagtag agcggcttgg ggtcatggtc gttgaccagg tgcagcgtct ggccgggctt
    10801 gagcgagtcg aagccgttga agatcatcgg gtgccgctcc cgcggggcga tctcgcgcac
    10861 atcgagccgg atcgtgtcga tcgttgcgtc agccatcatg cgtgtccttt cggtgccgtc
    10921 cccggcttgc gcagccggga tatcagtcgc ggtcgtcccg cgcttgccag ttgccagtgc
    10981 gcgccaacgc gcgccacgtt gccgaggaaa caggccagcc ccagcgtcgc ggccaggccg
    11041 gccagccggc tcagcagccg gtcgtcgagt gcgagcgcca gcatcgagcc ggccagcgcc
    11101 agcgaccaga gcgcccagcc cgcgcgggcc agccgcgagc tatacagctg ctccagccgt
    11161 ggcacccgct gccggccggc cagcggcgcg tagcggtgga gccagacgag gaaggtagcg
    11221 atcttataga agaagccctg gatcgccgtc agtgtcagac cggcgatcgc cagccagccg
    11281 gccgcgaccc agagcagcga gtccggcccg acgccgcttg cgatccccca gaccagcagc
    11341 gctcctgtac caaccccaca gccggccgag acgagcgcga acgggatatg gacatcaacg
    11401 ccccgtcgcc ggcggacctg gtagagatgg acgagctgga cgccgaagag gagttgccca
    11461 cctgccagca gcagcgcgcc ggccagcagg atcatgcgcg gagcctccgc gatcagcccg
    11521 attgccagcg tccacggccc gaggccggag agcgccagcg cgacccaggc gacacggtgc
    11581 cagagccggt cttcagagag ggtgaacatc ccgaccagcc ggtaggcgac gccactgagc
    11641 atgacggcca cccagccgcc cagcatcagc gtcgcgtggg cggccagtag tcgtagcgtc
    11701 agcgcgccga ggaagccggt tcccttgttg agtgccagta gcagcccgag ggcgacacct
    11761 ccgcccagcc cggccagcgc cgccacgatg tgccaggcga cgacatccat ccggggcgcc
    11821 cgcttgagcg tccaggcgat gacgacgaga tagacgatta gcccgctgaa gacgagcgtc
    11881 gcccccagtc ccagcgccgg ccgccaggtc gtcagcagcc caggcaggaa gagcaccaat
    11941 cctgacaagt acagccagaa cgagacgcgc cccagccgga ccgaggccag ctcggtctgc
    12001 aagacgaccg gcacgagctg atagctggcg ccgaggatga cggccgcgat gacgccgagc
    12061 gtgttcagat ggacgaacgc cagcagccgc ggggcataga acccatccag cagcagcgag
    12121 tacgcccagg gcgagaccgc ggcaacaacc gccagcccaa gcatccccag cgccatgaag
    12181 cgggccggca ggtcgagggc cggggcgtgg gcggagctga tgccggccga catcacgacc
    12241 cgccgtcgcc ggcagccggc ttctggatca gaatctcgac atgacccggt gtcagttcgc
    12301 gcgtctcgtg ccggtagcca agctcatcga gccggggata gaggtgcatc ggccgtcgca
    12361 ggtgatggac gtgcaggctg ccaccggccg gcaggtcttc aagcgcctcc aggatgcgga
    12421 tcatcggttc cggcggcacc agctcgctga cgtcgatcgt gatcgtggcg aaggccgcgt
    12481 cccagtcgag cggctggtcg tccgcgtgcg ttgccaccgg ttccggctgc cgctggcgcg
    12541 agcgcccggt gttgtagaag ctgatctccc agtcgtccgc tgcgtgccgc tcggcccagt
    12601 gctcgaatcc gcgcatgccc agcacgtcat agagcgggat cggatcgaac gtgttgcgca
    12661 gcaccagcac ctgccccggc ggcacctgcc ggacggcctc catgatcggc ccgaacggct
    12721 cctccccacg ccgttggtgc tcacggacat ccagctcgac ggcgaccggc gcggctccga
    12781 cccacggcgg cggggacgcc accggcgcat cggcagggtt cgcgaccgtc tcttcggcgt
    12841 ccggctccag cccgagatgc tggttgagtg tccgaaccag ttccggcggc tcgagcccgg
    12901 cgatccgggc ggcctgctcg accgtcacga ggcggctctg gacccgtcgc aggaccgggt
    12961 tacgcagctt gctgaaggcc gggtcggtcg caatgaatac gtccagcagg tccgggtaac
    13021 gctgcaggac ct
//
LOCUS       DASUGJ010000694         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1918157, whole genome shotgun sequence.
ACCESSION   DASUGJ010000694 DASUGJ010000000
VERSION     DASUGJ010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918157"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2039)
                     /locus_tag="VFV93_15790"
     CDS             complement(<1..>2039)
                     /locus_tag="VFV93_15790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX5166867.1"
                     /translation="LVLRGDFDGDPTFVDLLTRTRETALDADSHQDLPFERLVNELKL
                     ERDLSVNPLVQVFFIMQNLPRQDSELIGLKIEPVELQVATTKVDLTLVLHETPEGLEG
                     GFEYDTALFDQSAIERLAEHLTVLLKGIVADPDQRLSELPFLTDAERQLVVEDWNRTD
                     QPYETVATVSALFARQVAASPDAVALIDGERVLTYHALNVRANQLAAHLRELGVGPGA
                     LVGVCLDRSADLVITLLGILKAGAAYVPLDPSYPTERLALMLADADALALVTTRKLAD
                     HVPTETAAVIRLDADAAAIANRAPTSLPPLAAAEDLAYVIYTSGSTGRPKGVMLEHRN
                     VAAFFAGMDVVLEHDPPGVWLAVTSVSFDISVLELLWTLCRGFRVILQPELRDIVGQH
                     PDRSLARQVARHGVTHLQCTPSLARVLAEDPEGLVALRAVRCLLLGGEALPPELAARL
                     VGDGQGPTLLNMYGPTETTVWSTVHRVAPPVAGPIPIGRPIANTRVYVLDANGRPQPI
                     GVPGELYVGGDGVARGYLGRPELAAERFVTDPFGPEPDGRLYRTGDLARWRPDGVLEF
                     LGRADQQVKLHGFRIELGEVEAALREHPSVADVVAVVREDRPGDPRLVAYLVGADGTS
                     PPAVETLRASLGDRLPEYMAPAMYVMLDALPLTPNGKIDRRALPAPDLARVAGGA"
BASE COUNT          337 a          710 c          697 g          295 t
ORIGIN      
        1 tccgccccac cggccacccg ggccagatcc ggcgccggca aggcccgccg gtcgatcttg
       61 ccgtttggtg tcagcggcag ggcatcaagc atgacgtaca tggccggcgc catgtactcc
      121 ggcagccggt cccccagcga agcccgcaac gtctcaacgg ctggcgggga cgtgccatcc
      181 gccccgacga ggtacgccac caggcgcgga tcacccggcc gatcctcccg caccactgcg
      241 acgacgtcag ccacgctcgg gtgctcgcgc agggcggcct cgacctcgcc aagctcgatg
      301 cggaagccgt gcagtttcac ctgctggtcg gcgcgtccca ggaattccag gacgccgtcc
      361 ggccgccagc gggcgaggtc gccggtccga tacaggcggc cgtcgggctc cgggccgaac
      421 gggtctgtca cgaaccgttc ggcggccagc tccgggcggc cgaggtagcc acgggccacg
      481 ccgtccccgc ccacgtagag ctcgccgggg acgccgatcg gctggggacg gccgttcgcg
      541 tccaggacgt acacccgggt gttggcgatc ggccggccga tcgggatcgg cccggcgacc
      601 ggcggcgcca cgcggtggac cgttgaccag accgtcgtct cagtcgggcc atacatgttc
      661 aggagcgtgg ggccttggcc gtcgccgaca aggcgagcgg ccagctcggg cggcagcgcc
      721 tccccgccga gcaacaggca gcggaccgcc cgtaaggcca ccagaccttc gggatcctcg
      781 gccagcacgc gcgcgagcga gggagtgcac tgcaggtggg tcaccccgtg ccgtgcgacc
      841 tgccgggcca gcgagcggtc agggtgttga cccacgatgt cacgcagctc cggctggagg
      901 atcacccgga agccgcgaca cagcgtccac agcagctcga gcaccgagat gtcgaatgag
      961 acgctggtga cggccagcca gacacccggc gggtcgtgct ccaacaccac gtccatgccg
     1021 gcgaagaatg ctgccacgtt gcggtgctcg agcatcaccc ccttgggccg gccggtcgag
     1081 ccggacgtgt agatcacata cgccagatcc tcggcagcgg ccagtggtgg tagagaggtc
     1141 ggcgcccggt tggcgatggc cgcagcgtcg gcgtccagcc gtatgactgc ggccgtctcg
     1201 gtcgggacat gatcagccag tttgcgcgtg gtcacgagcg caagcgcgtc cgcatccgcc
     1261 agcatcaagg ccagtcgctc ggtcgggtag ctcgggtcga gcggcacgta ggcggcgccg
     1321 gccttgagga tgcccagcag cgtgatcacc agatcggccg agcggtccag gcagaccccg
     1381 accagtgcgc cgggcccgac accaagctcg cgcaggtggg cggccagttg gttcgcgcgt
     1441 acgttcagcg cgtgataggt cagcacgcgc tcgccgtcga tcagcgcgac cgcatccgga
     1501 gacgcggcca cctgtcgtgc gaacagcgcc gacacggtcg caacagtttc gtacggctga
     1561 tcggtccggt tccagtcctc gaccaccaac tgccgctcgg cgtcggtcag gaacggcagc
     1621 tctgacagcc gttggtcggg atcggccacg atccccttca gcagcaccgt caagtgctcc
     1681 gccagccgct cgatggcgct ctgatcgaac agcgccgtat cgtactcaaa gccaccttcg
     1741 agcccctccg gcgtttcgtg gagcacgagg gtgagatcga ccttcgtcgt ggcaacctgg
     1801 agctcaaccg gctcgatctt caacccgatg agctcagagt cctgccgtgg caggttctgc
     1861 atgatgaaga acacttgaac cagcgggttg acgctgaggt cgcgctcgag cttcaactcg
     1921 ttgaccaggc gctcaaaggg cagatcctgg tgggagtccg cgtcgagggc agtctcacgg
     1981 gtcctggtca gcaggtcgac gaaggtcggg tcgccgtcga agtcgcctcg caagaccag
//
LOCUS       DASUGJ010000695         7609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1363003, whole genome shotgun sequence.
ACCESSION   DASUGJ010000695 DASUGJ010000000
VERSION     DASUGJ010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363003"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VFV93_15795"
     CDS             <1..1221
                     /locus_tag="VFV93_15795"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166868.1"
                     /translation="DMPRFRPQMFASLRHRNYRLYWIGSLVANTGDWMDQIAIGWLIW
                     ELTGSSAYLGIMAFCRAFPILLFTLFGGAIADRFERRRVMQGTQSFAMVLALLLALLV
                     YTDVVQVWMVLAIAALRGVMLSVNLPTRQALLSDIVDRDHLSNAIALNSATLNLTRVF
                     GGSLGGLGITLVGVSGVFLLNGLSFIILIVALAMMTIPPTTAPKASKNVIRSIGEGLS
                     YVRHHTTLRSLTMLALVPMIFGMPYMSLMPVFADEVLGIGNEGYGLLVSLTGVGALCG
                     ALSVAALNDYRKRGRLMLFIMVGFGAMLLLFSRSSWPLVSGALLLGVGACQTGYMAFN
                     TTLLQTHASDEMRGRVMSVFFLNRGLVPLGTIGAGFAAEFIGAQWTVTIMASVVILLA
                     LLTIARVPQVREVE"
     gene            complement(1298..2785)
                     /locus_tag="VFV93_15800"
     CDS             complement(1298..2785)
                     /locus_tag="VFV93_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166869.1"
                     /translation="MRRVVQIAAMLAIVASVVAIPWVGAQTGSADAFQRTWARTDKPV
                     NDHQVDRTWMWGPQETAIETTEPYAEAPGGQRTVVYYDKSRMEDNSYRGSDPWDVTNG
                     LLVVELITGQMQTGDDTFVPRDPAGVNVAGDPNQTNPITYALLADLLDAPAATAGSAI
                     TARGTVTDSGIAIADDPSLAGKGVTAAWYVPDTDHTVASPFWTFMNSSGIVYEGDAFT
                     TEALFENPFYATGFPITEAFWANVTVNGTAKDVLLQCFERRCLTYTPDNAPGWQVEAG
                     NVGLHYYEWRHGEEGGGQTPSPTPTPDPNAIYQSNLTNWPVVTDNSGNSAYPTYEDYR
                     IRTVRGADMYAISDVALGEGSYRVRVSNVSGGDTTACLAIRAERHPTARTFPKRSTNF
                     CLIYENGQAREATAFIQTINSDNTTSTSTLGSWGLPTPIAAGDWTQLRIDAFGNDMTY
                     LVNGAQLGTSGGQSATGLLALASYSDDASHPVTVAFRDMLVRRTP"
     gene            complement(2948..3283)
                     /locus_tag="VFV93_15805"
     CDS             complement(2948..3283)
                     /locus_tag="VFV93_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166870.1"
                     /translation="MHGPPMYFTPNWQAAHRSVETLAALNPQVAVTGHGLPMAGDLLH
                     SDLNRLARDFEQLAVPVSGRYVDEPAVTNRTGVVSVPPAPPHAGPDGLVAFGVAALAT
                     MFLASRLRR"
     gene            complement(3320..3907)
                     /locus_tag="VFV93_15810"
     CDS             complement(3320..3907)
                     /locus_tag="VFV93_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5166871.1"
                     /translation="MNLDQTLNLPGRPSPTGNVTDDLSYLRTGIVNVCFYGQPGVGDR
                     GWVLIDTGMYGFSGRIMAEAARRFGENARPAAIILTHGHFDHVGALKTLANAWDVPVY
                     AHPLELPYLTDESPYPPPDPSVGGGAMSALSPLYPKGPFRIQPPPVPLPSDGSVPGMP
                     GWRWMQTPGHAPGHVALFREADRTLIAGDAFVTTQ"
     gene            complement(4106..7051)
                     /locus_tag="VFV93_15815"
     CDS             complement(4106..7051)
                     /locus_tag="VFV93_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5166872.1"
                     /translation="MDFAADPAEVMTTEDDLEAPQHYGLDPAEREEILSQFAALYPFP
                     LDQFQLEAGRAFLDGESVMVAAPTGTGKTILAELGVYEAFRRRGRVIYTTPIKALSNQ
                     KFRDLRALYGDQVGLLTGDVTENPRAPITVMTTEVLRNMLLQTPWEVDQVDCVIFDEV
                     HYIADQERGTTWEEAIILCPEHIQLICLSATVSNAEEIAAWISRTHRPIKLITHFERA
                     IPLALYYYLDKKLRPVIDHNGNQVGNYPNAGGEVRRKMGRGGITADQRRQAEMDEPQP
                     WEILLAMSAADMLPAIYFLFSRRDCEDYAQRFAMMKPNFIRDEATTTRINAVIDAHIG
                     SMREEDRQLEQVRLITSLASQGIGFHHAGLLPVLKQLVEVMFTRGLLQVVFATDTLAL
                     GVNMPARSVVIGRMTKWDGRSRRQLIPNEFQQMAGRAGRRGMDLKGNVVVPHSPWIKF
                     TEMMAIATGELEPVTSAFSIRYNTVLNLWDPPHGNRVRTMLQQSLAQFQSARRVRELE
                     EDILDLGARIHEIPQGCLIGLDAGDELLDDYRGLNHSVDALRSRERNVQSEVRLLNVR
                     ADARPWPEPGRQAIRRVFRAAEPGLVAHVRNEGWGVYLGKARAGGVGLFLFGTAVKLV
                     PEYRLVDYLPANNLSVPVPEALTMIDDPLDDATALIDPALVAATNDALHALPLPDLDA
                     QIAAYRTELEKRNESARQQQLEDLEAVRTQTEELIALRANHPCHTCPRRKEHQTNLKR
                     VAALERERGRVEQQLREEQQAEEERIRGIIGGIRNVLHRFNYLHRGYPTAKADLLADV
                     FDTNGLVICEMIDRGMVDTLQPADLAEVFSWFAFDRDSRFSNTYNLPNQLVLLRRRLE
                     DMEHQVLATERGNDLYISNGHNAGFYGAMRAWCNGATMSQITEQIDLSEGDLVLTFNK
                     TIDLMRQVREMIANVAPDHPLRETLTIAERFVKRDIVEQSLALGFLPIASEESDPAPD
                     DTADALDDE"
     gene            complement(7112..7516)
                     /locus_tag="VFV93_15820"
     CDS             complement(7112..7516)
                     /locus_tag="VFV93_15820"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEX5166873.1"
                     /translation="MLDAISRIQSHTTHGREDFDSDEMIRVWVIHHLQIIGEAARSLP
                     DDLRQRYPEVPWPQIIGMRHILVHHYFGLKWDEVWDTAERDLPVLKRAFEEIALELAK
                     KPKSNAADEPIGILRPNNRPSRRGFCAGHRQQ"
BASE COUNT         1121 a         2354 c         2707 g         1427 t
ORIGIN      
        1 gacatgcccc gcttccggcc gcagatgttc gcctcgctgc gccaccgcaa ctaccgcctc
       61 tactggatcg gctcgctggt cgccaacacc ggcgactgga tggaccagat cgcgatcggc
      121 tggctgatct gggagctgac cggctccagc gcctacctcg gcatcatggc cttctgccgc
      181 gccttcccga tcctgctctt cacgctgttt ggcggcgcga tcgccgaccg tttcgagcgc
      241 cgccgcgtca tgcagggcac gcagagcttc gcgatggttc tggcgctgtt gctggccctg
      301 ctggtctaca ccgatgtcgt ccaggtctgg atggtgctgg cgatcgctgc cctgcgcggg
      361 gtcatgctct cggtcaatct gccgacccgc caggcgctgc tctccgatat cgtcgaccgt
      421 gaccacctct caaacgcgat cgccctcaac tcggccacct tgaacctgac gcgggtcttc
      481 ggcggctcgc tgggcgggct gggcatcacg ctggtgggtg tctccggcgt cttcctgctc
      541 aacgggctgt cgttcatcat tctgatcgtc gcgctggcga tgatgacgat cccgccgacg
      601 acggcgccga aggccagcaa gaacgtcatc cgctcgatcg gcgaggggct gtcgtacgtc
      661 cgccatcaca cgacgctgcg ctcgctgacg atgctggcgc tggtgccgat gatcttcggg
      721 atgccgtaca tgtcgctgat gccggtcttc gccgacgagg tgctggggat cggcaacgag
      781 ggctacggcc tgctggtctc gctgacaggc gttggcgcgc tgtgtggggc gctgagcgtc
      841 gcggcgttga acgactaccg caagcgcggg cggctgatgc tgttcatcat ggttggcttc
      901 ggtgcgatgc tgctcctctt ctcgcgctcc tcgtggccac tggtctccgg cgcgttgctg
      961 ctgggtgtcg gcgcctgcca gaccggctac atggccttca acacgacact gttgcagacg
     1021 cacgcctccg acgagatgcg cgggcgggtg atgagcgtct tcttcctgaa tcgcggcctc
     1081 gtcccgctcg gcacgatcgg cgccggcttc gcggccgagt tcatcggcgc ccagtggacc
     1141 gtcacgatca tggcctcagt cgtgatcctg ctggccctgc tgaccatcgc ccgcgtcccc
     1201 caggtgcgcg aggtcgagta ggggcgtcga cgtatgcgcg gtcgacgtgc cttatctccc
     1261 cggactgcgg ctggaacttc ttcccgattc cagccgccta cggcgtcctc cgcaccagca
     1321 tatcgcggaa ggcgacggtg acggggtggc tggcgtcgtc gctgtagctg gcgagggcga
     1381 gcaggccggt ggcgctctgg ccgccggagg tgccgagctg ggcgccgttc accaggtagg
     1441 tcatgtcatt gccgaaggcg tcgatgcgca gctgggtcca gtcgccggcg gcgattgggg
     1501 tggggagccc ccagctgccg agcgtgctgg ttgatgtcgt gttgtcggag ttgatcgtct
     1561 ggatgaaggc ggtcgcctcg cgggcctggc cgttctcgta gatcaggcag aagttggtcg
     1621 agcgcttcgg gaaggtgcgc gccgtcgggt gccgctctgc gcggatcgcc aggcaggcgg
     1681 tcgtgtcgcc gccgctgaca ttgctgaccc gaacgcggta gctgccctcg ccgagcgcga
     1741 cgtcgctgat cgcgtacatg tcggcgccgc gaacagtccg gatgcggtag tcctcatagg
     1801 tcgggtaggc ggagttgccg gagttgtcgg tgacgaccgg ccagttggtc aggttcgact
     1861 ggtagatcgc gttcgggtcg ggcgtcggcg tcggcgatgg cgtctgaccg ccgccctcct
     1921 cgccgtggcg ccactcgtag taatggaggc cgacgttgcc ggcctcgacc tgccagccgg
     1981 gggcgttgtc gggcgtgtag gtcaggcagc ggcgctcgaa gcattgcagg aggacgtcct
     2041 tggcggtgcc gttgacggtg acgtttgccc agaaggcttc ggtgatcggg aagccggtcg
     2101 cgtagaacgg attctcgaag agcgcctcgg tcgtgaacgc gtcgccctcg tagacgatcc
     2161 cggacgagtt catgaacgtc cagaacggcg aggcgacggt gtggtcggtg tcgggcacgt
     2221 accaggcagc cgtcacgccc ttgccggcca gcgacggatc gtccgcgatg gcgatgccgg
     2281 agtcggtgac ggtcccacgg gcggtgatgg cgctgccggc cgtcgcggcg ggggcgtcga
     2341 gcagatctgc cagcagcgcg taggtgatcg ggttggtctg gttcgggtca ccggcgacgt
     2401 tgacgccggc cgggtcgcgc gggacgaacg tgtcatcacc ggtctgcatc tggccggtga
     2461 tcagttcgac gaccagcagg ccgttggtaa catcccacgg atcgctgccg cggtagctgt
     2521 tgtcttccat ccgtgacttg tcgtagtaga cgacggtccg ctggccgccc ggcgcttcgg
     2581 cgtatggctc ggtcgtctcg atcgccgtct cctgcggccc ccacatccag gtccggtcga
     2641 cctggtggtc gttgactggc ttgtcggttc gcgcccaggt gcgctggaag gcgtcggcgc
     2701 taccagtctg cgcgccgacc cacggtatcg ccaccacact cgccacgata gccagcatgg
     2761 cggcgatttg aacgacacgt ctcatagata gtgctcccat tgtcccgcgc ggcgattgtt
     2821 gccacgtctc agtctgcggg gatcaagggt acatgacggg gtgcgggttc gtgacgcgtg
     2881 acgcgtgacg cgtcgatgtg gttacggtat cgttccgtcc cgcgtcccgc gtcacgcgtc
     2941 acgtccctca tctccgaagg cgcgaggcga ggaacatcgt ggcgagtgcc gcgacgccga
     3001 aggcgaccag gccgtccggg ccggcgtggg gtggggccgg gggaaccgag acgacgccgg
     3061 tgcggttagt gacggccggc tcgtccacgt agcggccgga gacgggaacg gcgagctgct
     3121 cgaaatcgcg ggcaaggcga ttgagatcgc tgtgcaggag gtcgccagcc atcggcaggc
     3181 cgtggccggt gacggcgacc tgtgggttga gcgcggccag cgtctcgacc gatcggtggg
     3241 cggcctgcca gttgggcgtg aagtacatcg gcgggccgtg catctcaagc cgctgggtgt
     3301 aggcgctgat gagcgattcc tactgggtcg tgacgaaggc gtcaccggcg atcagtgttc
     3361 ggtcggcctc gcggaagagg gcgacatggc cgggcgcgtg gccgggggtc tgcatccagc
     3421 gccagcccgg cattcctggc accgagccgt cggagggcag cgggactggc ggcggttgaa
     3481 tccggaacgg tcccttcggg tagagcggcg agagcgccga catcgcgccg ccaccgaccg
     3541 agggatcggg cggtgggtac ggggattcgt cggtcaggta gggcagttcc agcgggtggg
     3601 cgtagaccgg cacgtcccat gcgttcgcca gcgtcttgag cgcaccgacg tggtcgaagt
     3661 ggccgtgcgt gaggatgatc gcggccggtc gggcgttctc cccgaagcgc cgggctgcct
     3721 cggccatgat ccggccgctg aagccgtaca tgccggtgtc gatcagtacc cagccgcgat
     3781 cgcccacacc cggctgcccg tagaagcaca cgttgacgat gccggtccgc aggtagctga
     3841 ggtcgtcggt gacgttcccg gtcgggctgg gccggccagg cagattgagc gtttgatcga
     3901 ggttcatctg ccggtctccg tccttgtgcg tctggtgctg ggatcgctga acggtcatcc
     3961 tgtgccgcag ggtgacgcgt gcaagggatg tgccagatga ttggaaatca ggcacgggaa
     4021 cgcaggatgt tcgttgtcag atacaggtct caattgagcg ggaggaagta ttcgccacgc
     4081 ccgaatcccg aatccggttc ccgccctact cgtcgtccag cgcgtcggcc gtgtcgtccg
     4141 gggccgggtc ggattcttcg ctggcgatcg ggaggaagcc gagggcgagc gattgttcga
     4201 cgatgtcgcg cttgacgaag cgttcggcga tcgtgagcgt ctcgcgcaat gggtggtcgg
     4261 gggcgacgtt ggcaatcatc tcacgcacct ggcgcatcag atcgatcgtc ttgttgaagg
     4321 tcaggacgag gtcgccctcg gagaggtcga tctgctcggt gatctgcgac atcgtcgcgc
     4381 cgttgcacca ggcccgcatc gcgccgtaga agccggcgtt gtggccgtta gagatgtaga
     4441 ggtcattgcc tcgctcggtc gccagcacct gatgctccat gtcctccagc cggcgacgca
     4501 gcaggacgag ctggttaggc aggttgtagg tgttggagaa gcgcgagtcg cggtcgaacg
     4561 cgaaccagga gaagacctca gccaggtcgg ccggttgcag ggtgtcgacc atgccgcgat
     4621 cgatcatctc gcagatgacg aggccgttgg tgtcgaagac atcggccagc aggtcggcct
     4681 tggcggtcgg gtagccgcgg tgcaggtagt tgaagcggtg cagcacgttg cggatgccgc
     4741 cgatgatgcc gcgaatgcgc tcctcttcgg cctgctgctc ctcgcggagc tgctgctcga
     4801 cccggccccg ctcccgttcg agggccgcca cccgcttgag gttggtctgg tgctccttgc
     4861 gacgcgggca agtgtggcag gggtggttgg cgcgcagcgc aatcagttct tcggtctggg
     4921 tccggacggc ttccaggtcc tccagctgct gctggcgggc ggattcgttg cgcttctcca
     4981 gttcggtccg gtaggcggcg atctgggcgt cgaggtcggg cagcggtagt gcatggaggg
     5041 cgtcgttggt ggccgcgacc agggccggat cgatcagcgc cgtcgcgtcg tccagcggat
     5101 cgtcgatcat cgtcagcgcc tcggggaccg gaacgctgag gttgttggcc ggcaggtaat
     5161 cgaccagccg gtactcagga acgagcttga cggccgtgcc gaacaggaag aggccgacgc
     5221 caccggcacg ggccttgccc aaatagacgc cccagccttc gttgcggacg tgggcgacca
     5281 gccccggttc ggcggcgcgg aagacgcggc ggatcgcctg ccggcccggc tccggccagg
     5341 ggcgggcatc ggcgcgcaca ttcagcagcc ggacctcgct ctggacgttg cgctcgcggc
     5401 tgcgcagggc gtcgaccgag tggttgagcc cccggtagtc gtccagcagc tcgtcgcccg
     5461 cgtcgaggcc gatcaggcag ccctgcggga tctcgtggat gcgggcgccg aggtcgagga
     5521 tgtcctcctc cagctcgcga acgcggcggg ccgactggaa ctgcgccaat gactgttgca
     5581 gcatcgtccg aacgcggttg ccgtggggag ggtcccagag gttgaggacg gtgttgtagc
     5641 ggatcgagaa agcactggtg accggctcca gctcgccggt cgcgatcgcc atcatctcgg
     5701 tgaacttgat ccacggcgag tgcgggacga cgacgttgcc cttcagatcc atcccgcgcc
     5761 gtccggcccg gccggccatc tgctggaact cgttggggat cagctggcgg cgcgagcgac
     5821 cgtcccactt cgtcatccgg ccgatgacga ccgagcgggc cggcatgttg acgcccagcg
     5881 cgagcgtgtc ggtcgcgaag acgacctgca gcagcccgcg ggtgaacatg acctcgacga
     5941 gttgcttcag caccggcagc agcccggcgt gatggaagcc gatcccctgg ctggcgagtg
     6001 acgtgatcag gcgcacctgc tcgagctggc gatcctcctc gcgcatcgat ccgatgtggg
     6061 cgtcgatgac ggcgttgatg cgggtcgtcg tcgcctcgtc gcggatgaag ttgggcttca
     6121 tcatcgcgaa gcgctgcgcg tagtcctcgc agtcgcggcg ggagaagagg aagtagatcg
     6181 caggcaacat gtcggcggct gacatcgcca gcaggatctc ccacggctgc ggctcgtcca
     6241 tctcggcctg gcggcgctgg tcggcggtga tgccgccgcg tcccatcttg cggcgcacct
     6301 cgccaccggc gttcgggtag ttgccaacct ggttgccgtt gtggtcgatg acgggccgca
     6361 gcttcttgtc gaggtagtag tagagcgcca gcggaatcgc ccgctcgaaa tgcgtgatga
     6421 gcttgatcgg acgatgcgtg cggctgatcc aggcggcgat ctcttcggcg ttcgagacgg
     6481 tcgccgagag gcagatgagc tggatatgct cggggcagag gatgatcgcc tcttcccagg
     6541 tcgtgccgcg ctcctgatcg gcgatgtaat gcacctcatc gaagatgacg cagtcgacct
     6601 gatcgacctc ccacggcgtc tgcaacagca tgttgcgcag cacctcggtc gtcatcaccg
     6661 tgatcggcgc gcgcgggttc tcggtgacat cgccggtcag cagcccgacc tggtcgccgt
     6721 agagcgcgcg caggtcgcgg aacttctggt tcgagagcgc cttgatcgga gtcgtgtaga
     6781 tgacgcggcc ccgccgccgg aatgcctcgt agacgccaag ctcggccagg atcgtcttac
     6841 cggtcccggt cggggcagca accatgaccg actcgccgtc gagaaaggcg cggccggcct
     6901 ccagctgaaa ctgatcgagc gggaatgggt agagcgcggc gaactggctg aggatctcct
     6961 cgcgttcagc ggggtcgagt ccgtagtgct gtggcgcttc gagatcatcc tcggtcgtca
     7021 tgacctcggc ggggtcggct gcaaagtcca ttgctgttcc aactgtactg gacgctcgcg
     7081 gccgtgggtc cgaactggtg ggccggcggc gtcactgctg acgatgtccc gcgcagaaac
     7141 cgcgccggga cgggcgattg ttgggccgga gtataccaat gggctcatcg gccgcgttcg
     7201 actttggctt cttagccaac tccagtgcga tctcctcgaa tgctcgcttg agcacgggta
     7261 aatctcgctc ggcggtgtcc caaacctcgt cccatttcag gccgaagtaa tggtggacga
     7321 gaatgtgacg cataccgatg atctgcggcc acggtacttc ggggtaacgt tgtcgaagat
     7381 cgtcgggcag cgaacgagcc gcctcgccga tgatctggag gtgatggatg acccagactc
     7441 ggatcatctc gtcgctgtcg aagtcctcgc gtccatgcgt ggtgtgcgac tggatccgtg
     7501 agatcgcgtc gagcacgtct gtaacgcgct cgtgatcgcc tctcacagtg gaaccgcttc
     7561 tgacaacacg cgttcgcgga tgcgctcttt taggccacca tcgacagcg
//
LOCUS       DASUGJ010000696         3719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_668343, whole genome shotgun sequence.
ACCESSION   DASUGJ010000696 DASUGJ010000000
VERSION     DASUGJ010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668343"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2757)
                     /locus_tag="VFV93_15825"
     CDS             complement(<1..2757)
                     /locus_tag="VFV93_15825"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5166874.1"
                     /translation="MSIHHLQTNVTPAGQVLGSAALHLDLLGEFAVWLDGRLVPDTAF
                     PRRKAISLLKLLALQPGYQLHREQVLDLLWPDLEPGSAGSQLYKAIHHARQAFTTVAP
                     SVAAETVLSLQGERLSLAAPGGVSTDVSAFEDEARSAIAERDADLLQRVAARYSGDLL
                     PGDLYEEWTIQRRQSLRDTAIDVLIELGEIYHSIGALGDSADALRRVIAMDPSREAAH
                     RLLMLVYARQGNRALALRQFRICSEAVDLELGAPPSRETLELYHDILDERIQRVVAQR
                     HAPAGELAVLPPLAGRQPELRLISDLLDQLAQGHGTVLAIEGDAGIGKTRLVQELLRH
                     ARRRHAHVFLGNAYEHEDQIPYQPFVEALRHALWSSPNDAELIPAELARAIPELPATA
                     ETVGIADQVASQGALFAGVLRFLAARATSAPVVVILEDLHAADDGSLKLFHYLARETA
                     QLPLLLIGSWRSNEPGAPQALPRVVGELDRGKRLQRISLTPLSVDEHRLLLDRALDAG
                     RVEPILADDVYRRSEGNPLFASELLHQLIRDGRLVQRGDVWRMRDAPGEPDSASTLTI
                     PHSLRALAQRRLELLSSGSRRLVQLAAVIGRDVQYPLLESGLHRDPIGDGSFLDAIDE
                     VLSAGLLVESGVELRFPHPLLREAIYEQLPRPRRTMWHDTVAGALEDLHADQPEQLPV
                     DAIAHHYRLAGNVDRAVQYLIRAVDQAERVYDHDGALRRLTDAQSLLAEPRSPDREAT
                     LSNVLERTGDVERAIGYVDRSLAAYQDALSALDSAGMQADRAHRFALHRKIGLAAILN
                     VNMPVAAEHLALARDLVGDAPLERARLLIAESLSDWHALQFEAAITSAARALALAEQA
                     DARIEVSQACEMLAIAHMPIGDWEASLQYELRRQSDDWSPEIVVAFDAHLCLYQYKLH
                     TEAAL"
     gene            complement(2965..3312)
                     /locus_tag="VFV93_15830"
     CDS             complement(2965..3312)
                     /locus_tag="VFV93_15830"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEX5166875.1"
                     /translation="MQPKLWTFRIRHILQSIEKIQRYTAGTSYEAFVVNDMAVDAVIR
                     NFEIIGIATQQIPDEVTSRYQDIPWAKMRGMRNVLAHQYEDVRLDVVWDTIQREFPDL
                     VPRLTAIYEQETS"
     gene            complement(3302..>3719)
                     /locus_tag="VFV93_15835"
     CDS             complement(3302..>3719)
                     /locus_tag="VFV93_15835"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5166876.1"
                     /translation="PDQRIPATPHGDHLRVNRRDVRSSCNLTNSEQGQAALEGSLMDL
                     NDVLSIIAAHEDELRRRHVATLSVFGSVARGEARPDSDIDLLIEFDGSPVTLFDLGGL
                     QVYLSEILGAEVDLVMRSSVIAELRDRIYNEAVRAA"
BASE COUNT          704 a         1173 c         1209 g          633 t
ORIGIN      
        1 aagcgccgct tcggtatgga gcttgtactg gtagaggcag aggtgagcgt cgaaggcgac
       61 gacgatctcc ggcgaccagt cgtccgactg gcggcgcagc tcgtactgga gcgacgcctc
      121 ccagtcgcca atcggcatgt gggcgatcgc cagcatctca cacgcctggc tcacttcgat
      181 gcgcgcgtcg gcttgctcgg cgagtgccag cgcgcgagcg gcactggtga tcgccgcctc
      241 gaattgcagc gcgtgccagt cgctcaacga ctcggcgatc agcagccgcg cgcgctcaag
      301 cggcgcatcc ccgaccagat cacgcgccaa tgccagatgc tcggcggcga ccggcatgtt
      361 gacgttcagg atcgccgcaa gtccgatctt gcgatgcaac gcgaagcgat gggcgcgatc
      421 tgcctgcatg ccggcgctgt cgagcgctga gagcgcgtcc tgatacgcag ccaggctccg
      481 atcgacgtag ccgatcgcgc gctcgacgtc gccggtgcgc tccagcacgt tcgacagcgt
      541 tgcctcgcgg tccggcgagc gcggttcggc cagcagcgac tgggcgtcgg tgagccggcg
      601 cagcgcgcca tcgtgatcgt agacgcgctc ggcctgatcc acggcccgga tcaggtattg
      661 aacggcgcgg tcgacgttcc cggccaggcg atagtgatgc gcaatcgcat cgaccggcaa
      721 ctgctccggc tggtccgcat gcaggtcttc gagtgctccc gcgacggtat cgtgccacat
      781 ggttcgtcgt gggcgtggga gctgctcgta gatcgcctca cgcaacagtg gatgtggaaa
      841 gcgcagctcg acgccggact cgaccagcag ccctgccgag agcacctcat cgatggcgtc
      901 cagaaacgat ccgtcaccga ttggatcgcg atgcagccct gactccagca gcggatactg
      961 aacgtcgcga ccgatcacag ccgcgagttg caccagccgg cgcgagccag acgagagtag
     1021 ttccaaccgg cgttgagcca gcgcccgaag agagtggggg atcgtcagcg tgctggccga
     1081 atcgggttca cccggcgcat cgcgcatgcg ccagacatcg ccacgctgga ccagccggcc
     1141 gtcacggata agctggtgca acagctcgct ggcgaacaac gggttgccct cggatcggcg
     1201 atagacatcg tcagccagta tcggttccac ccggccggcg tcgagcgccc gatcgagcag
     1261 gagacgatgc tcgtcgaccg agagcggtgt caggctgata cgctgaagcc gcttcccacg
     1321 gtcgagttcg ccgacgacac gcgggagtgc ctgcggtgcg ccgggttcgt tcgatcgcca
     1381 gctaccgatc agcagcaacg gtagctgcgc ggtctcgcgt gccagatagt ggaacagctt
     1441 gagcgagccg tcgtcggccg cgtgcaggtc ttcgaggatg acgacgaccg gtgcggacgt
     1501 cgcccgcgcg gcgaggaagc gcagcacgcc ggcgaagagc gcaccctgcg aggccacttg
     1561 gtccgcgatc ccaaccgtct cggccgtcgc tgggagctcg gggatcgcac gagccagttc
     1621 agccgggatc aactctgcgt cgttcgggct gctccagagc gcatgacgca acgcctcgac
     1681 gaagggctga tacgggatct ggtcttcgtg ctcgtaggcg ttgccgagga agacgtgtgc
     1741 gtgacggcgt cgggcatgcc gaagcagctc ctgcaccaag cgcgtcttgc cgatccccgc
     1801 gtcaccttca atggccagga cggtgccgtg tccctgcgcc agttgatcga ggagatcgct
     1861 gatgaggcgc agctccggct gccggcccgc cagcggcggg aggacggcca attccccggc
     1921 aggcgcgtgg cgctgggcca cgacgcgctg gatgcgttca tcgagaatat cgtgatacag
     1981 ctccagtgtc tctcgcgacg gaggtgcgcc cagctcgaga tcgaccgcct ccgagcagat
     2041 ccggaattga cggagcgcga gcgcacggtt gccctgtcgc gcatagacga gcatcagcag
     2101 ccggtgggcc gcctcgcgac tcgggtccat cgcgatcact cgccgcagcg cgtcggcgga
     2161 gtccccgagc gcgccgatcg aatggtagat ctcgcccagt tcgatcagga catcgatggc
     2221 tgtgtcacga agcgactggc gtcgctgaat cgtccactcc tcgtagaggt cgcccggcaa
     2281 cagatcgccg ctgtatcgcg ctgcgacccg ctgaagtaga tcggcgtcgc gctcggcgat
     2341 cgcgctccga gcctcgtctt cgaacgccga cacatcggtc gagacgccac cgggcgccgc
     2401 gagcgagagg cgctcaccct ggagtgacag gaccgtctcg gccgcgaccg acggcgcgac
     2461 agtcgtgaac gcctgtcgcg catgatggat cgccttgtag agctgcgagc cagcagaccc
     2521 cggctccagg tccggccaga gcagatcaag cacctgttca cgatgcaact ggtagcccgg
     2581 ctgcaacgcc agcagcttga gcaggctgat cgccttgcgg cgtggaaacg cagtatccgg
     2641 aaccagccgc ccgtcgagcc agacagcgaa ctcgccgagc agatccagat gcagcgcggc
     2701 tgaaccgaga acctgccccg cgggcgtgac gttcgtctgg aggtgatgga tagacatccc
     2761 tcccccaaat ctgacagcga atgtaccccc agcataccga agatcgatcg aatgtgcagg
     2821 tttaccggaa gcgacaacgg attgctgccg atccagaggg aaggcagcgc gttcaatgga
     2881 gtacggacgc gccgctcatg gccgacgcat ggaatcgcct gcgccgggat atctgtgagg
     2941 cgtcaaacgg cccagatgat tgaactacga cgtttcctgt tcgtagattg ccgtcaagcg
     3001 aggaaccaga tcaggaaact ctcgttggat cgtatcccac accacatcga ggcgaacgtc
     3061 ttcatactga tgcgcgagca cattgcgcat ccctcgcatc ttcgcccaag gaatatcttg
     3121 ataacgtgag gtgacttcgt ctgggatctg ttgcgttgca attccgatga tctcaaagtt
     3181 gcggatgaca gcgtcaaccg ccatgtcatt gacgacaaac gcttcgtacg acgtgccggc
     3241 agtgtatcgc tgaatcttct cgattgattg caggatatgg cgaatccgga acgtccagag
     3301 tttaggctgc acggactgct tcgttataga tgcgatcacg caattcagcg atgacggagc
     3361 ttctcatgac gaggtcgacc tcggcgccca ggatttcgct caggtacacc tgtaaaccgc
     3421 caagatcgaa cagcgtgacg ggcgaaccgt caaactcgat cagcaggtcg atgtcgctgt
     3481 ccgggcgagc ttcgccgcgc gcgactgagc cgaagacgga aagagtcgcg acgtgacggc
     3541 gacggagttc gtcctcgtgg gcggcgatga tcgacaagac gtcattgagg tccatgaggc
     3601 tgccttccag tgccgcttga ccctgttcgc tgttcgtcag gttacatgat gatcgaacgt
     3661 cgcgtcgatt cacacgcaaa tgatccccgt gaggtgtggc agggatgcgc tggtcgggc
//
LOCUS       DASUGJ010000697         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_807658, whole genome shotgun sequence.
ACCESSION   DASUGJ010000697 DASUGJ010000000
VERSION     DASUGJ010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807658"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1566
                     /locus_tag="VFV93_15840"
     CDS             307..1566
                     /locus_tag="VFV93_15840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166877.1"
                     /translation="MHQRLGAYAVYLIYSAAFALFASVTWTVNLVYQVDRVGLSPFQI
                     VLIGTALELSYFLFEVPTGIVADVYSRRLSVIIGTFMLGAGFVLMGAVPEFGVMLLSQ
                     VICGIGYTLLSGAQEAWIADEIGEERARPAFLRGSQAATVGTITGIPIAIGLGSLDLA
                     LPIIVGGAGIVVLGGFLLLAMQETGFHGAAREDFTGWQSMTNTFHAGTRMIRSRPVLL
                     TIIGIAVVFGSFNEGFDRLSVPHLLENFSFPAIGGFSDVVWVGGIRFVVELLGIVVLE
                     VVRRRVDLGSHRAVSGTLFAINGGLVVSVLVFGLAGSSALAIVMMIVASLFRRSIYPL
                     ETVWVNQSLDPKVRATVISMTSQGDSFGQIFGGPAVGAVGSLFGLRAAIVTAGGLLTP
                     ILLLYARALGQGSEAVITVDDAAIVAE"
     gene            1864..>2051
                     /locus_tag="VFV93_15845"
     CDS             1864..>2051
                     /locus_tag="VFV93_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX5166878.1"
                     /translation="MVINASLAGVIEAARGSTEIGGILGARRGVEGILTGDFVDLSEL
                     DDDRLERLRRTPSAALGT"
BASE COUNT          294 a          623 c          719 g          415 t
ORIGIN      
        1 gaccggctgg gtcgcccgtg ccggctggcg cacgacgacg gaagacggcc agtcggtctg
       61 gcatctgcgc tttgagacga ttgacgacag cgctgagcgc tgagtgctga gtcgatcgcc
      121 gggcgagacc acggccgtcg ccccagcaag ggaacaaccc aacactcggc actcagcact
      181 cagcactttc aactcgtaag ggaggctccc cgtgctacat tgcaagttca tcgtcaaccc
      241 cgacaccctg gatgctgtcg cctggcctgg gcggcggcgt atcgaatgtg gagttgatgg
      301 aacttggtgc atcaacggct gggagcatat gcggtctacc tgatctacag cgcggcgttc
      361 gcgctcttcg catcggttac ctggacggtc aacctcgtct atcaggttga tcgggtcggg
      421 ctgagtccat ttcagatcgt cctgatcggg acagcgctgg agctgtccta ttttctgttc
      481 gaagtgccaa ccgggatcgt tgccgatgtc tacagccggc ggctgtcggt catcatcggc
      541 acgttcatgc ttggcgcggg cttcgtcctg atgggggcgg tgccagagtt cggcgtgatg
      601 ctgctctcgc aggtcatctg cgggatcggc tacacgctgc tgagcggggc tcaggaggcc
      661 tggatcgccg acgagatcgg cgaggaacgg gccaggccgg ccttcctgcg tggctcacaa
      721 gcggcgacgg tagggaccat caccggtatc ccgatcgcga tcgggctcgg cagcctcgat
      781 ctggcgctgc cgatcatcgt cggtggcgca gggatcgtcg tgctcggtgg cttcctgctg
      841 ctggcgatgc aggagaccgg ctttcacggc gcggcccgtg aggacttcac cggctggcag
      901 tcgatgacga acacgttcca cgccggaaca cggatgattc gcagccggcc ggtcctgctg
      961 acgatcatcg gtatcgccgt cgtattcggc agcttcaacg aaggtttcga ccgcctctcg
     1021 gtgccgcacc tgctggagaa cttctcgttt ccggccatcg gcggcttctc ggacgtggtc
     1081 tgggtcggcg ggattcgctt cgtcgtcgag ttgctgggta tcgtcgtgct ggaggttgtc
     1141 cggcggcggg tcgacctggg tagtcatcgg gccgtgtcgg ggacgctgtt cgcgatcaac
     1201 ggcggtctgg tcgtctcggt cctggtcttc gggctggccg gtagctcggc gctggcgatc
     1261 gtgatgatga tcgtcgcttc gctgttccgt cgcagcatct acccactgga gacggtctgg
     1321 gtcaatcaga gtctcgatcc gaaagtccgg gcgaccgtca tctcgatgac gagtcagggc
     1381 gactcgttcg gccagatctt cggcggtccc gccgtcggcg ctgtcggctc gctgttcggc
     1441 ctgcgggcgg cgatcgtgac ggccggcggg ttgctgacac cgattctgct tctgtatgcc
     1501 cgcgcgctcg gtcagggcag cgaagcggtc atcacggtcg acgacgcggc gattgtggcg
     1561 gagtgacctc accccccaac ccgccaccca gagtcccctc tccgcgtcgg ccgggtaccc
     1621 tctgggtcgc gggttggggg gtgaggtccg ttttcctcac cttgcgtcta caccaccaac
     1681 ctgcttagaa tcgtcgggcg cgtgctggcc gtggggaggc cgctcgcatt tggttgggga
     1741 tgggtggacg cccgctgtgg gcgcgaacct cggctgcttc gccctcactg cggtgggggc
     1801 ggaccgagga gcggaaagat cggagccacg acgctgctgg tcgcccagtc gggcgggccg
     1861 acggtggtca tcaatgccag cctggctggc gtcattgagg ccgcacgagg ctcgactgag
     1921 atcggcggga tccttggggc gcggaggggc gtcgagggga tcctgaccgg cgatttcgtt
     1981 gatctttccg aactggatga cgaccgtctc gaacgactgc ggcggacgcc gtcggcggcg
     2041 ctcgggacga g
//
LOCUS       DASUGJ010000698         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1363514, whole genome shotgun sequence.
ACCESSION   DASUGJ010000698 DASUGJ010000000
VERSION     DASUGJ010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363514"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..756)
                     /locus_tag="VFV93_15850"
     CDS             complement(250..756)
                     /locus_tag="VFV93_15850"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5166879.1"
                     /translation="MNDGLIDGFRHHIWATRQVMALCRDLTDDQLRSNATGTFGSIID
                     TFWHTISSDAGYYARLAGEEPAWDRRADDPPSLEQMSHYLDDLDGRWNRFLETPFDAE
                     RTFAINWEDDSVRDVPAGVVLMQALHHGTEHRAHICTILTSLGIAIPDLGLWDWAEAT
                     NRATLRVA"
     gene            complement(877..1794)
                     /locus_tag="VFV93_15855"
     CDS             complement(877..1794)
                     /locus_tag="VFV93_15855"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX5166880.1"
                     /translation="MKVHLDTDFGGDIDDLAALALLLNWPDVEITGITTVADEDGRRA
                     GYARYVLGLAGRGEIPVAAGADVASGSFRWRPTYPPEDAFWPEPVVPAPGPLDAALEL
                     LRQSIDAGAIVIGIGPYTNLALLDRRSPGILRQTDLYLVGGFIYPPRPTRSHWGNDVD
                     YNVQMDIDAAQHLLTHARPTLVPLHVTVETTLRHAQIPRLASANPVSALIARQALAFE
                     AAGEITAIDRDRCVDIPADFINHLYDPLGCAVALGWSGAVIEELPLALTVEDGWLHER
                     VSDTGTPTRVVSRANGAAFDALWLDLVCT"
     gene            complement(1802..2191)
                     /locus_tag="VFV93_15860"
     CDS             complement(1802..2191)
                     /locus_tag="VFV93_15860"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5166881.1"
                     /translation="MTDGAIQLVREWHDAVNNGDTEGLAGLVTDDVEVGGPRGSAHGR
                     DVLIDWVARTGIRMEPTDWYQRGETVIVCQRAAWPGEDGMPGVPQKVSSVFTFRDGRI
                     AGVARYGDLVTAFREAGLAETDRVDVE"
     gene            2279..2833
                     /locus_tag="VFV93_15865"
     CDS             2279..2833
                     /locus_tag="VFV93_15865"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5166882.1"
                     /translation="MIETTVTGNGAGEHCSEGTMHQQPFDVQAEESGRVNAPAATAYR
                     LIADYTTHHPAILPPAITNLTVEQGGYGEGTVISFDVRLGGRSRRMRARISEPSEGVL
                     LETDLDTGGVTTFIVTPDGDASRVTIRTEFTSAAGLTGWIERRFAPGMLRKLYRQELA
                     NLDTYARQQGDAAAPLNDPAPVTG"
BASE COUNT          544 a         1007 c          994 g          494 t
ORIGIN      
        1 gttcctgagg accaagctcg cctgatccgg tttcgcaatc aatcccaaat gcccaatccg
       61 gtacgggcga tatcgcaata tcgccccctg catccccacc ggagcgttcg ggcgggacac
      121 gaagggcgat tttgcgaaat cgcccgtacc ggattgggga ttgaatatgg gggattgtcc
      181 ccaagaacat ggtggcatgt tgcctcccca tggagacgac ctgccgcaag agagttcgaa
      241 tgctccgagc tacgccactc gcaacgtcgc gcggttcgtc gcttcggccc agtcccagag
      301 gcccaggtcg gggatcgcga tgccgagcga tgtcaggatc gtgcagatgt gggcgcggtg
      361 ttcggtgccg tggtgcagcg cctgcatcag caccacacca gccgggacat cgcgaacgga
      421 gtcgtcttcc cagttgatcg cgaaggtgcg ctcggcgtcg aatggcgtct ccagaaagcg
      481 attccagcgc ccgtcgaggt cgtcgaggta gtgcgacatc tgctccaggc tgggcgggtc
      541 gtcggcgcgg cgatcccagg ccggttcttc gccggccagc cgcgcgtagt agcccgcgtc
      601 ggacgagatc gtgtgccaga acgtgtcgat gatgctgccg aacgtcccgg tcgcgttcga
      661 gcgaagctgg tcgtcggtca ggtcgcggca gagcgccatc acctgccgcg tcgcccagat
      721 gtgatgtcgg aagccgtcga tcaagccatc gttcatctgt cttcctctca cgtgttgcgt
      781 tctccgggaa agcgcacgcg ttgatcaggc aacagcgtga tgccgatcac cttcgctcca
      841 atgtggcgat accagtacgc tggcaatgat aggcgatcag gtacagacca ggtcaagcca
      901 caacgcatcg aacgcagcac cattggccct gctgacgacc cgcgttggcg tgccggtgtc
      961 gctgacccgc tcgtggagcc agccgtcctc aacggtcagg gccagtggta gctcctcaat
     1021 gacggccccg gaccagccga gcgcgacggc gcagccaagg gggtcgtaga ggtggttgat
     1081 gaaatcggca gggatatcaa cgcagcggtc gcggtcgatc gcggtgatct cgccggccgc
     1141 ctcgaaagcg agcgcctggc gagcgatcag cgcgctgacc ggattcgcgg acgccagccg
     1201 tggaatctgc gcgtgccgca acgtcgtctc gaccgtcaca tgcagcggca cgagcgttgg
     1261 tcgcgcgtgg gtcagcagat gctgcgcggc gtcgatgtcc atctggacgt tgtagtcgac
     1321 gtcgttgccc cagtgcgacc gggtcggcct cggtggatag atgaagccgc caaccagata
     1381 gaggtccgtc tgccgcagga tgccggggct gcgccggtcg agcagcgcca ggttcgtgta
     1441 tgggccgatg ccgatcacga tcgcgccggc gtcgatgctt tgccgaagca gctccagcgc
     1501 cgcgtccagc ggaccgggag caggcacgac cggctccggc cagaatgcat cctccggggg
     1561 ataggtcgga cgccagcgga aagacccgct cgcgacatcg gcgccggccg cgaccgggat
     1621 ctcgccacga ccggccagcc ccagcacgta gcgcgcatag ccggcccgac ggccgtcctc
     1681 gtcggcgacg gtcgtgatgc cggtgatctc aacgtccggc cagttgagca gcagcgccag
     1741 cgcggccagg tcgtcgatgt cgccgccgaa gtcggtgtcg aggtggacct tcaccggtgc
     1801 ctcactcgac atcaacgcgg tcggtctcgg ccagcccggc ctcccggaac gcagtgacga
     1861 gatcgccgta gcgcgcgacg ccggcgattc gaccgtcccg gaaggtgaag acggaactga
     1921 ccttctgtgg cactccaggc ataccgtcct cgcccggcca ggcggcccgc tggcagacga
     1981 tgacggtttc gccgcgctgg taccagtcgg tcggctccat cctgatcccg gtccgcgcga
     2041 cccagtcgat cagaacatcc cgaccgtggg ccgagccacg cggaccaccg acctcgacat
     2101 cgtcggtgac gagacccgct agcccctcag tgtcgccgtt gttgaccgcg tcatgccatt
     2161 cgcggaccag ttggatcgca ccgtccgtca tcgcttcgat ctccttcgcc ggtgaccgta
     2221 tcaaatggac agtcgccgcc ccaacggccg gacagactcc tggacggcgg agaggcgtat
     2281 gatcgagacg accgtcaccg gcaacggcgc cggtgagcat tgttcggagg ggaccatgca
     2341 ccaacaaccg tttgatgtcc aggccgagga atcgggtcgc gtcaacgcac cggccgcaac
     2401 ggcctaccgg ctgatcgcgg actacaccac ccaccatccc gccatcctgc ctccggcgat
     2461 caccaacctg accgtcgaac agggtggcta tggcgagggc actgtcatca gcttcgatgt
     2521 ccggctcggt gggagatcgc gccggatgcg ggcgcgcatc agcgagccga gcgaaggcgt
     2581 cctgctggag accgatctcg ataccggcgg cgtcaccacc ttcatcgtca cgccagacgg
     2641 tgatgccagc cgcgtcacga tccggaccga gttcacctcg gcagccgggc tcactggctg
     2701 gatcgagcgc cgctttgcgc ccggcatgct ccgcaagctc taccggcagg aactggcgaa
     2761 tctcgacaca tacgcccgac agcaggggga cgccgcagcg cccctgaacg acccggctcc
     2821 agtcaccggt taacacacgg atgccgcgtg ccttgtgcgc gatgcacaag gcacgcggcc
     2881 gaatcgtgct gccgcgcaca taccatgtcc ggacactggc gcgcacaatt ggcgacgtac
     2941 gcgaaacgcc gccgcaggtg agtcatcaga acctcatggg cgcggtgttc gacgacgtgg
     3001 tttgtgcgca gggcagcaag gagtgaatgt gagcggaag
//
LOCUS       DASUGJ010000699         4345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1363770, whole genome shotgun sequence.
ACCESSION   DASUGJ010000699 DASUGJ010000000
VERSION     DASUGJ010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363770"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VFV93_15870"
     CDS             <1..1307
                     /locus_tag="VFV93_15870"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5166883.1"
                     /translation="ARGASVKMTASAEPGDGRSADETIDHAALNELSSELVQVASVNG
                     LLRYVNSAWRRALGYGDQDISRILMLDVIHPNHQNSFAETMRSVIQQQAAHNVSTVLL
                     TRTGEPLSVSGTMDCQVAARRPVLLRAVLRDAQSDANLDLLSGQWTSIFESPAIGMSV
                     QSLDGRWLAVNDAMAEILGYSPNELIAMTFHDITHPDDIAENAERLQRLVIGAERRFQ
                     MEKRYIHRDGSIVTAILTVSIVTDRAGNPLRFVSQIQDITARTQLETQLLHKASHDVL
                     TGLPNRALFIDRLEHALQRLQRDNGQIAVMFLDLDGFKAVNDQYGHDQGDRLLVEIGR
                     RLRACARAGDTVSRLGGDEFTILLEQVRHPSIATEVAQRVRETLAYPIMLGEHVTTIS
                     PSIGIVISSGAGDTAEAILHDADTAMYQAKRAGKDRAVLAER"
     gene            1408..2586
                     /locus_tag="VFV93_15875"
     CDS             1408..2586
                     /locus_tag="VFV93_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEX5166884.1"
                     /translation="MAGSSNAGERVRREDLQGTYAMEAERQMEGADNTERQEMLLAYG
                     LPSAESIRDRKISLFTRGRWRFNAGSFMNTEYLEDMRKLDGQDVVFLGAPYDGGTTYR
                     PGTRFGPQAMRKISPQASGYNPGMGVDLHESLTMVDAGDITIIPSNIEKSFDQIAKAI
                     SYVAEREVFPIVLGGDHAIGYPDVRGLAPFIDGNIGIIHFDRHSDLSEYSLDERMHGS
                     PFFHATNIPNAPAANLVQIGIGGWQGSKDGIKIARERSATVITIDDVDRFGPQRIVEY
                     ALEIAWKNAKAVWISFDVDSLDPAYTPGTGTPVPGGLTPREALKMIRDIAAEGLVGME
                     VVEVSPPYDVQDHTALMGVHAILECLGSLVKHNKLGKQRVPPAERTDQVSPMDMPPKG
                     "
     gene            3084..4262
                     /locus_tag="VFV93_15880"
     CDS             3084..4262
                     /locus_tag="VFV93_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5166885.1"
                     /translation="MPASAQTPSSASDPNPKPMGIETDQTQPLGGATWRVYGYQEGLV
                     GHTTANGHVIQPEDFFVALPCFCVLSSNGGNEFQVRLDYKGKSITVPVWDVGPWNVSD
                     NYWDPPEQREWQGLEQGMPMATAAYYQNYNNGNDGWGRKVTSPGGIDIGDGAFHALGM
                     TGSDWVDVTFLWLEPKRWELAALPAPYENITTVWWAEQPPLGRAEVNTSGNFGYIWET
                     GHNVPNPMINLYWANGGWRRYGMPISEFFREVSVDGTVRFVQYFERAVLTLDLTDPNA
                     PAVHQEDIGYGTYIDPNAAKPVEVYEPHPNGRFFPETGHTLQNGFKAYWEQNGGLDVF
                     GMPLSEEWSETNAEGRKVVMQVFERARLEWWPDRVGTDEEITRGLLGVELLQRRGLLP
                     "
BASE COUNT          863 a         1374 c         1378 g          730 t
ORIGIN      
        1 cggcaagagg tgcgagcgtg aagatgacgg cgagcgctga accaggtgat ggcaggtcgg
       61 cagacgagac gatcgaccac gccgcgctca acgagctctc ctcggaactg gttcaggtcg
      121 cgtcggtcaa tggcctcctc cgctacgtca acagtgcctg gcgtcgcgcg cttgggtacg
      181 gcgatcagga catctctcgc atcctgatgc tcgacgtgat ccacccgaat caccagaact
      241 ccttcgctga aaccatgcgc tccgtcattc agcaacaggc cgctcacaac gtcagtaccg
      301 tcctgctgac tcgaaccggc gagccgctgt cggttagcgg gacgatggat tgccaggtcg
      361 cagcccgccg gccggtgctg ctgcgggccg tactgcgcga tgcccaatca gacgcgaatc
      421 tggacttgct cagcgggcaa tggacgagca tcttcgagag tccggcgatc gggatgtcgg
      481 tccagtcgct cgatggccgc tggctggccg tcaacgacgc gatggcagag atcctgggct
      541 attcgcccaa cgagctgatc gcgatgacgt tccacgacat cacccatccg gacgacatcg
      601 ccgagaacgc tgagcggttg cagcggctcg tcatcggcgc cgagcgccgc ttccagatgg
      661 agaaacgcta catccatcgg gacggcagca tcgtcaccgc catcctgacc gtctcgatcg
      721 taaccgaccg tgccggcaac ccattgcgtt tcgtctcgca gattcaggac atcactgccc
      781 gaacacaact cgaaacacag ctgctacaca aggcatcgca cgacgtgctg actgggctgc
      841 cgaaccgggc gctgtttatc gaccggctcg aacacgcgct gcaacgcctg cagcgcgaca
      901 acggccagat cgccgtcatg ttcctcgatc tggacggctt caaggctgtc aacgaccagt
      961 acggccacga ccagggagac cggctgctgg ttgagatcgg ccggcgtctg cgcgcctgcg
     1021 cgcgggccgg cgacaccgtc tcccggctcg gtggcgacga gttcacgatc ctgctcgaac
     1081 aggtccgcca cccatcgatc gcgactgagg tcgcccaacg tgtgcgtgag acactggcct
     1141 acccaattat gctcggcgag catgtcacga cgatctcgcc gagcatcggg attgtcatct
     1201 cgtccggtgc cggcgacacg gccgaagcga tcctgcacga cgccgacacc gccatgtacc
     1261 aggccaagcg cgccggcaag gaccgcgccg tcctggccga gcggtaggct gtgggccacc
     1321 gcagatggcc tatcacccca tcaccgatcg tagtaccctg cacgtagcgg caatctgtgg
     1381 gtgatttcgg acgcgaggga gcgggagatg gcaggtagca gcaacgccgg ggagcgggtc
     1441 aggcgcgaag acctccaggg aacctacgcg atggaggcag agcgccagat ggagggggcc
     1501 gacaacaccg agcggcagga gatgctgctg gcctacggcc tgccctcggc cgagagtatc
     1561 cgcgaccgga agatcagcct gttcactcgt ggccgctggc gcttcaacgc cggcagcttc
     1621 atgaacaccg agtacctgga ggacatgcgc aagctcgacg ggcaggatgt cgtcttcctc
     1681 ggtgcgccct acgacggcgg cacgacctac cggcccggta cccgcttcgg cccacaggcg
     1741 atgcgcaaga tctcgccgca ggctagcggc tacaaccccg gcatgggtgt cgacctgcat
     1801 gagtcgctga cgatggtcga cgccggcgat atcaccatca tcccatcgaa tattgagaag
     1861 agcttcgacc agatcgcgaa ggccatctca tacgtcgccg agcgcgaggt cttcccgatc
     1921 gtgctcggcg gcgatcacgc gatcggctac cccgacgtgc gcgggctggc gccgttcatc
     1981 gacggcaaca tcggcatcat ccacttcgac cggcactcag acctctccga atacagcctc
     2041 gacgagcgca tgcacggctc gccgttcttc cacgctacca acatccccaa cgcaccggct
     2101 gccaacctgg tccagatcgg catcggcggc tggcagggat cgaaggacgg catcaagatc
     2161 gcccgcgagc gcagcgcgac cgtcatcacg atcgacgatg tcgaccgctt cggcccgcag
     2221 cgaatcgtcg agtacgcgct ggagatcgcc tggaagaatg ccaaggccgt ctggatctcg
     2281 ttcgacgtcg actcgctcga tccagcctat acgcccggaa ccggcacacc ggtccccggc
     2341 gggctgacgc cgcgcgaggc gctgaagatg atccgcgata tcgccgccga gggcctggtc
     2401 gggatggaag tcgtcgaagt ctccccgccc tatgacgtcc aggaccacac ggcgctgatg
     2461 ggggtccacg cgatcctcga gtgcctcggt tcgctggtca agcacaacaa gctcggcaag
     2521 cagcgcgtcc cacccgccga acggaccgac caagtctcac cgatggacat gccgccgaaa
     2581 ggttagatcc tacgaactca gttcccgagg gacgccccga ccagattgag actgggatct
     2641 caatctgatc ggggcatatt gcgtactcaa atgatcgtcg tcgaaggatg cacgtgacgt
     2701 cgggagggta attcgtcccg gtgttcaccg tgctcccagt acgcactgat gagacgcgtc
     2761 caatcggccg tggttcgtga cagtctgtca cgggcgatga tcccaatgtg tacggacaga
     2821 gtgcatcgag cagtgccgaa atggcgcaca agatcgtcaa aatcgctgac atggcgtcaa
     2881 agttgctcga aattggtcac catgatattc tgcgtcgtac ccggaggttc actgacgctt
     2941 atgtcgcggt ggggcgcgag cgagtgttcg gtgactgatc ggttgtgtga aggtggggat
     3001 aattggcgaa gtacgatgtg gggcgccgcc ggtcccggat tgtcgcaaca ctgctgacaa
     3061 ttgcgagtat ggtgttgctg gttatgccgg cgagtgcaca gacaccatca tcggcgagcg
     3121 atccgaatcc aaagccgatg gggatagaga cggaccagac gcaacctctc ggtggagcga
     3181 cctggcgtgt ttatggttac caggaagggc tggtcggcca tacaaccgcc aatgggcacg
     3241 tcattcagcc ggaagacttc ttcgtcgcac tgccatgctt ctgcgtcctg tccagcaacg
     3301 gtggcaacga gttccaggtt cggctcgatt acaagggtaa gagcatcacc gtcccggtct
     3361 gggatgtcgg tccgtggaac gtcagcgaca attactggga cccgcccgaa cagcgcgagt
     3421 ggcaggggct cgaacagggc atgccgatgg cgacggcggc ctactatcag aactacaaca
     3481 acggcaacga cggctggggc cgcaaggtca cgtcgccagg cgggatcgac atcggggacg
     3541 gcgcgttcca cgcgctcggg atgaccggct ccgactgggt tgacgtgacg ttcctctggc
     3601 ttgagccgaa gcgctgggag ttggccgcgc tgccggcgcc gtacgaaaac atcaccaccg
     3661 tctggtgggc cgagcaaccg ccactcggtc gcgccgaggt gaacaccagc ggcaacttcg
     3721 gctatatctg ggagacgggc cacaacgtcc cgaacccgat gatcaacctc tactgggcta
     3781 acggcggctg gcggcgctac ggcatgccga tcagcgagtt cttccgtgag gtgagcgtcg
     3841 atgggaccgt gcgcttcgtg cagtacttcg aacgcgccgt gctgaccctt gacctgaccg
     3901 acccgaacgc gccggccgtc catcaggaag atatcggcta cggaacgtac atcgatccga
     3961 acgcggccaa gccggtcgag gtctatgagc cacatccgaa cgggcggttc ttccctgaga
     4021 ccggccacac actccagaac ggcttcaagg cctactggga gcagaacggc gggctggatg
     4081 tgttcgggat gccgctctcc gaggagtggt cagagacgaa cgccgagggc cgcaaggtcg
     4141 tcatgcaggt cttcgagcgg gcgcggctgg agtggtggcc ggaccgtgtc ggcaccgacg
     4201 aggagatcac ccgcggcctg ctcggagtcg agctgctcca gcggcgcggg ctgcttccgt
     4261 aaccaacaca ccagggcaga agcgagtaac gcgggtgaca gaaacggtcg cccgcgttgt
     4321 gtgttgtatt caggaacagg ttgct
//
LOCUS       DASUGJ010000700         4273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_669115, whole genome shotgun sequence.
ACCESSION   DASUGJ010000700 DASUGJ010000000
VERSION     DASUGJ010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669115"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..918
                     /locus_tag="VFV93_15885"
     CDS             22..918
                     /locus_tag="VFV93_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5166886.1"
                     /translation="MASNQPPAATPAQPYGEIYDLGYKHYDGKRLGRTHAIQALIVYS
                     IKRGLGIKKKWTAKIIPFVLYMIAFVPAFIVAGIKAFVPSDDFTYNYHDLNQFISLTL
                     FIFAAALGPEMLSDDRRENVLGLYFSRALTRLDYLLSKIAAMGILLGTIAFGPPLLLY
                     IANVLLADNPISYFFDHLDDIGRIVAFGTLVSTYLGAISLAIAAYTNRKGVAGAIFIG
                     GLLMITGIANALFQALDTGLRDYIILLSPVDMIPALSGWVWSEDVNIDNIQLDGIWYG
                     LAVVATVTLSALIMYRRYLADA"
     gene            943..1944
                     /locus_tag="VFV93_15890"
     CDS             943..1944
                     /locus_tag="VFV93_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166887.1"
                     /translation="MSVPALPTTSEAPPQASISPPISEQSTIVVQGLSKWYGDVVAVS
                     DVTFGIEPGVTALLGPNGAGKSTTLEMLTGLLAPSAGTIRILGKPARGDVTLYQRIGL
                     VPEQETLYPFLTAREFVRLNAVLQKLPNPDAAAERALGIVEMLPDADRDLRGFSKGMR
                     QRTKVAAALVHDPDVLLMDEPLNGTDPLQRARLINLITRLGQQGKTVVVSSHILHEVE
                     RFADRILVINRGKLAAAGDFHAIRDKIDEHARAVRVRCSDPRRLASELIRDASLVSMR
                     FEPSLDSDLPSLVAETADVRAFYRSVPNVARQLDIHLYEIAALDDSLASVFAYVVGR"
     gene            1948..2703
                     /locus_tag="VFV93_15895"
     CDS             1948..2703
                     /locus_tag="VFV93_15895"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5166888.1"
                     /translation="MATARSMLDPTPILGLTLRQFIGGKSIRIVFLFSLVPALFSLIF
                     MLDSGDLTSREFFAGIFQEFIAPTLLPIATLILATNALGNEVEDRTMVYLVLKPISRM
                     RIVLEKFAAVALTATLLLWEGTLLAYLLAMRGDAGNNIDQLLAICFAQLIGVLGYGAL
                     FMAISLIVPRALLAGLIYALLWESLFGRFIPGIRLVSVRHYVQSIYVRAIDNPAIDLD
                     EAMRFVPALIVIALLVVASLAITTWRLQTMNLE"
     gene            2825..3229
                     /locus_tag="VFV93_15900"
     CDS             2825..3229
                     /locus_tag="VFV93_15900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX5166889.1"
                     /translation="MSAQLSDAVRAFLEEGPRFCVLATINADGTPQQSVMWYELRGDT
                     IMMNTEASRLKARNLARDARASICVEEAYKYVTISGTVSLDATHETSQADIYALARRY
                     NPDFKEGDYADFANQQRVTINLSIDKVVSNHL"
     gene            3278..4033
                     /gene="cofE"
                     /locus_tag="VFV93_15905"
     CDS             3278..4033
                     /gene="cofE"
                     /locus_tag="VFV93_15905"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX5166890.1"
                     /translation="MPSEIRVVGLDGIPEVTPGDDLVEVIAGALTASGETLQAGDILV
                     VTHKIVSKSEGRLVDLATIEPSPFASDYAERYGKDARQVEVVLRESDRIVRMGHGVMI
                     CETRHGFICANAAVDASNVPGDHHVCLLPIDPDASARKIRDGLKARFGVAPAVIISDS
                     FGRPWRKGIVNVAVGVAGMSPFADYRGQTDPYGYDLRVSVMAVADELAATAELLAGKT
                     DARPVALIRGYHYTEGDGTAADLVMDPERDLFR"
BASE COUNT          737 a         1433 c         1314 g          789 t
ORIGIN      
        1 ccgaagagat ggtggccgcc aatggcgagt aaccagccgc cggccgcgac gccggcccag
       61 ccatacggcg agatctacga tctcggctac aagcactacg acggcaagcg tctcggccgg
      121 actcacgcga ttcaggcgct gatcgtctac tcgatcaagc gcgggcttgg catcaagaag
      181 aagtggacgg cgaagattat cccgttcgtc ctctacatga ttgccttcgt gccggccttc
      241 atcgtcgccg gaatcaaggc attcgtgccg tcagacgact ttacgtacaa ctaccacgac
      301 ttgaaccagt tcatcagcct gacgttgttc atcttcgccg ccgcactcgg cccggagatg
      361 ctctccgacg accggcgcga gaacgtgctc ggtctctact tctcgcgggc gctgacgcgg
      421 ctggactatc tgctctctaa gatcgcggcg atgggcatcc tgctcgggac gatcgcgttc
      481 ggtccgccgc tgctgctcta catcgccaac gtcctactgg cggacaaccc gatcagttac
      541 ttcttcgacc atctcgacga catcggcagg atcgttgcat tcgggacact ggtctccacc
      601 tacctcggcg cgatctcgct ggcgatcgcc gcctacacga accgcaaggg cgtcgccggc
      661 gcgatcttca tcggcggtct cctgatgatt accggcatcg ccaatgccct ctttcaggcg
      721 ctcgacaccg gcctgcgcga ttacatcatc ctattgtcgc cagttgacat gatcccagcc
      781 ctcagcggct gggtctggag cgaagacgtc aacatcgaca acatccagct tgacggcatc
      841 tggtacgggc tggcagtcgt cgcaaccgtc acgctgtccg ccctcatcat gtaccggcgc
      901 tatctggccg atgcgtaatc gagtggaaca ggggttctgt ctgtgagcgt cccggccctg
      961 ccaacgacca gcgaggcgcc tcctcaggcc tcgatctctc cgcccatctc cgagcagtcg
     1021 acgatcgtcg tccaggggct gtcgaagtgg tacggtgatg tcgtggctgt ctcggatgtc
     1081 acgttcggga ttgagcctgg cgtaaccgcg ctgctcgggc ccaacggtgc cggcaagtcg
     1141 acgacgctgg agatgctgac cggcctgctg gcgccatcgg cgggcacgat ccgcatcctc
     1201 ggcaagccgg cacgcggcga tgtgacgctc taccagcgga tcggcctcgt gccggagcag
     1261 gaaacgctct acccgttcct gacggcccgc gagttcgtcc ggctcaacgc cgtcctccag
     1321 aagctgccga atcccgacgc ggcggccgag cgggcgctcg gcatcgtcga gatgctgccg
     1381 gacgccgacc gcgatctgcg cggcttctcg aagggcatgc gccagcggac gaaggtcgcg
     1441 gcggcgctcg tccacgatcc cgatgtgctg ctgatggacg agccgctaaa cggcaccgat
     1501 ccgctccagc gcgcccggct gatcaacctg atcacccggt tgggccagca gggcaagacg
     1561 gtcgtcgtct cgtcccatat cctgcacgag gtcgagcgct tcgcggaccg catcctcgtc
     1621 atcaaccgag gcaagctggc cgccgccggc gacttccacg ccatccgcga caagatcgac
     1681 gagcacgccc gggctgtccg cgttcgctgc agcgatccgc gccggctggc gagtgagctg
     1741 atccgcgacg ccagcctcgt ctcgatgcgc ttcgagccgt cgctggacag cgatctgccg
     1801 tcgctcgtcg ccgaaacggc tgacgtccgc gcgttctacc ggagtgtccc gaacgtcgcg
     1861 cgccaactcg atatccacct gtatgagatc gcggcgctgg acgactcgct ggcgagtgtc
     1921 ttcgcctatg ttgtggggcg ctgattgatg gcaactgctc gctcgatgct cgatccgaca
     1981 ccgatcctcg gcctgaccct gcggcagttc atcggtggca agtcgatccg gatcgtcttt
     2041 ctcttctcac tcgtcccggc gctcttctcg ctgatcttca tgctcgactc gggcgacttg
     2101 acgtcgcgcg agttcttcgc cggcatcttc caggagttca tcgcgccgac gctgctgccg
     2161 atcgcgacgc tgatcctggc gaccaacgcg ctcggcaacg aggtcgagga ccggacaatg
     2221 gtttatctcg tccttaagcc aatcagccgc atgcggatcg tccttgagaa gttcgcggcc
     2281 gttgccctca cagccacact gctgctctgg gagggaacgc tgctggcgta tctgctggcg
     2341 atgcggggcg acgccggcaa caacatcgac cagctgctgg cgatctgctt tgcgcagttg
     2401 atcggcgtcc tcggctacgg cgcgctcttc atggcgatca gcctgatcgt cccacgcgcg
     2461 ctgctggccg gcctgatcta cgcgctgctc tgggagagcc tgttcggccg cttcattccc
     2521 ggcatccggc tcgtcagcgt ccgccactac gtccagtcga tctacgtccg tgccatcgac
     2581 aaccctgcca tcgacctcga cgaggcgatg cgcttcgtac cggccctgat cgtgatcgcg
     2641 ctgctggtcg tcgcgtcgct ggccatcaca acctggcggc tacaaacgat gaacctggag
     2701 tagagcgcca accccgaccg gaataccgcg ctcgaacaat ggttcaatac gctgagcggg
     2761 caaccggccg gggggcggtt gccgcatctc acccgcgaag atggattgtg gacagggaga
     2821 ctggatgtca gcacagctca gcgatgccgt acgcgcgttc cttgaagaag ggccgcgctt
     2881 ctgcgtgctc gcgacgatca acgccgatgg cacgccgcag cagtcggtta tgtggtacga
     2941 actacgcggc gacacgatca tgatgaacac cgaggccagc cgcctgaaag cccgcaacct
     3001 cgcgcgcgac gcccgtgcct cgatctgcgt cgaggaagcg tacaagtacg tcacgatcag
     3061 cggcaccgtc agcctcgacg ccacccacga aacctcccag gccgacatct atgccctggc
     3121 ccgccgctat aatcccgact tcaaggaagg cgactacgcc gacttcgcca accagcagcg
     3181 tgtcaccatc aacctctcga tcgacaaggt cgtttcgaat catctctaac gatcggccgt
     3241 tagcggccga tcgccgagtg catgtggagc ttccaggatg ccgagtgaga ttcgtgtcgt
     3301 cgggctggac ggtatccctg aggtgacgcc cggggatgat ctggtcgagg tcatcgccgg
     3361 ggcgctgacc gccagcggcg agacgctgca agcgggcgac atcctcgtcg tcacccacaa
     3421 gatcgtctcg aaatccgaag ggcggctggt tgatctggca acgattgagc cgtcgccgtt
     3481 cgcaagcgat tacgccgaac gctacggcaa ggacgcgcgt caggtcgagg tcgtcctgcg
     3541 cgagagtgac cgtatcgttc ggatgggcca tggcgtcatg atctgcgaga cgcggcacgg
     3601 cttcatctgc gccaacgccg ctgtcgacgc gtcgaacgtg ccgggcgatc accacgtctg
     3661 cctgctgccg atcgatccgg acgcgtccgc gcggaagatt cgcgacgggc tgaaggcacg
     3721 cttcggcgtc gcgccggccg tcatcatcag cgactcgttc ggccggccgt ggaggaaggg
     3781 gatcgtcaac gtcgccgtcg gcgtggccgg catgagtccg ttcgccgact accgcggcca
     3841 gaccgacccc tacggctacg acctgcgcgt cagcgtcatg gccgtcgccg acgaactggc
     3901 cgcgaccgcc gagctgctgg ccggcaagac cgacgcccgc ccggtcgccc tgatccgcgg
     3961 ctaccactac actgaaggcg acggcaccgc cgccgacctg gtgatggacc cggagcgcga
     4021 cctgttcagg tagagccgga ggagatccaa tccaataatc aatccggtac gggcgatatc
     4081 gcaatatcgc ccttcttgat gcccggcgcg tggaaaaggg cgatattgca atattgcccg
     4141 taccggattg attattggat tgggtgctga tggtgggtca gtggacatcg acacgaacgc
     4201 ggtggtagcc ggtcgcgccg tcgggcaggg tgccgcgttc ctcggatgtc tggagctcgc
     4261 cggtgccgtc ggt
//
LOCUS       DASUGJ010000701         7476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1502167, whole genome shotgun sequence.
ACCESSION   DASUGJ010000701 DASUGJ010000000
VERSION     DASUGJ010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502167"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..531
                     /locus_tag="VFV93_15910"
     CDS             310..531
                     /locus_tag="VFV93_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166891.1"
                     /translation="MTTMTTTPTPSQETIDQFVTFCHGHLAGVQEMLAADPTLINTRS
                     SLDETCQVSSWPRRSTTLRSTCQSFLRLE"
     gene            589..2721
                     /locus_tag="VFV93_15915"
     CDS             589..2721
                     /locus_tag="VFV93_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166892.1"
                     /translation="MNEQDRPHQPGGIGRHLPALAIFVAATVLLTWPLASRMDDTFIS
                     WGDPVFQSWTIAWDWHALKTDPLGIFDANVFYPWGNTLAYSDHLFGQALTVLPVIGLT
                     ENGILADNIATLLAFFFSALAMYLLVYDMTGNRLAGILAGVAYAFAPSRVAHLEHLHL
                     LSAQWPPLMLLCLRRAVCRDAGRGTRDAVEEDRSPGPPYPALSTQHSARNPRPASRVP
                     RPGPWFLGLGACFFAQGLFGVYFLYFSIVMLIIAGAVYAAFALYDRDWQVMRALGLAG
                     LTCAFAGALLIPTLLPYWQVNQELDVERTTAEVNAWSAEPSDYLAVWPRAKLWNDLLQ
                     RNHRDVERDLFPGLFIIALALIGLTHREAGRQRWVLLAVAVGGVVFSFGLSATIAGRD
                     VPLPYRIFYDWMPGFRAIRVPARLGLLALVGAGGLAGLGIDRLWRLGRDELPLFRWYP
                     HPLAQRPRLVGSAVATLALVFILLETLPRMELPDPLPAPPDNPPPAYAWIRDNPAPTI
                     ELPMGEGLVGSAWPNFWSMFHWNETVNGYSGIVPPSYYPFREEMRAFPSEKTIRLLQG
                     IGVENVIIHSDFPDRDRATLDAELAAHPELTLALAGPDAVYRLAPDPWMWDLADAIPS
                     DAIVDLPNAGADPPTYGLLMAILQRTDHDVAGQGQIDYYTFTPPDSTTCYAILADSDD
                     PAAYGYPAARPVLQEGGLTLYVRPDCGG"
     gene            2782..3000
                     /locus_tag="VFV93_15920"
     CDS             2782..3000
                     /locus_tag="VFV93_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166893.1"
                     /translation="MAVQRSADHDPADDIQVVEISREEGIAMFDRQARERLGLSGEEF
                     LRKWNAGEIEDPDRNDVMMLVMMIPFTK"
     gene            complement(3550..4446)
                     /locus_tag="VFV93_15925"
     CDS             complement(3550..4446)
                     /locus_tag="VFV93_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166894.1"
                     /translation="MGRQVGGRPGVAAALACCVLMLFSMVAVAQEDGDPLLENWQRTD
                     KPVMDGVINRTWMWGPQSSAHMTWEAYADSPDGQRKVIYFDKSRMEITDPSADRSDPW
                     FVSNGLLVVELVSGQLQTGDAQFENRGPANINVAGDADDPNGVTYATMANLRVPYPGP
                     EPGPLTWTVDRAGIVGTDARFAAYDVGVTWFAPETGHWVAEPFWAFMTGTGTVWDDER
                     YQTEPLFPDPFYATGYPITEAYWVRVLLKGQPKDVLLQCFERRCLTWTPDNPAGWEVE
                     AGNVGQHYYLWRYGTPPPRPVG"
     gene            complement(4644..5438)
                     /locus_tag="VFV93_15930"
     CDS             complement(4644..5438)
                     /locus_tag="VFV93_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166895.1"
                     /translation="MARIFAGEFSMIDQEVLDTLKQLPDSFWIFAEFDIGRNVDWFVI
                     RPDAPSTLILTELKRIAKPIRGAVNGIWEKLNGGGDWVELPTNGSDTNPYWQAVNSAN
                     AMADWLWNNQQLYRSTADIRPATEFRVWPDLLLLSPPGTTHRLPLAPPSRYGRWWFNT
                     DDWLRHVRGWTPRTGVTLSPSEIGNLAEALRLTEIWSGGDIAPPALPPIQTTPTGDTA
                     ALFTNWLVGIDQRITRLENELAWYREREREREQCQGQLSEILGDRS"
     gene            5812..6810
                     /gene="fgd"
                     /locus_tag="VFV93_15935"
     CDS             5812..6810
                     /gene="fgd"
                     /locus_tag="VFV93_15935"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HEX5166896.1"
                     /translation="MLTLGYKASAEQFGPRELLEFSVIAEEAGFDSVVVSDHFQPWRH
                     THGHSPFSFAWLAALGERTSRVRLGTSVVTPTFRYHPSIVAQAMGTLGCLAPGRVFLG
                     VGTGESLNEIPSIGIEWPDFKERFARLRESITLMQRLWTEDRVTFDGTYYQTRNATVY
                     DRPEEGIPLYIAASGATAARLAGRVADGFICTSGKAPELYSETLVPAVQEGAGKAGRD
                     ASKIEMTIEMKVSYDHDKQRAMEDTKHWAALALSPDEKTGVEDPLEMERLADALPVER
                     AASRWIVSNDPDEQVEKIKTYIDLGFTHLIFHAPGPDQERFIRLYGKEVLPRLRAL"
     gene            complement(6864..7358)
                     /locus_tag="VFV93_15940"
     CDS             complement(6864..7358)
                     /locus_tag="VFV93_15940"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5166897.1"
                     /translation="MAVAVDQLLYLLDEAFTGNDWHSLLGNLRDITDDEWCWTPPRGS
                     RSLRDMVEHIGSCKIMYEDYAFGDASLGWEDPVVTGGVALESVATALAWLREAHERLR
                     YRVSRLDDVDLDQRRLTNWGEQRETRWIIAVMIQHDLYHAGEINHLRALYRGDDRWEH
                     ERDA"
BASE COUNT         1277 a         2482 c         2332 g         1385 t
ORIGIN      
        1 ctctccggcc cagagcagcc ggaagtcgtg gtgtcgcagc gcaacggtcg cccgttcgcc
       61 accttgccat tcatcctgtc tgtcgtccat ccgcgagcgc ccgcactcct tccagcagcg
      121 gtgtggggtc cctcgctcac ggttcggtgt cgaatgacac caacacaggt agtcaaggtg
      181 aacagctggg tccacggttg ttctgcagtg ttttgtacca caggagtcag ggccggtgcg
      241 tgctggcgac gttgacggga ggtgcctttg cgcgcatgag cggcacagac aggatatcga
      301 cgaacactga tgacgacgat gacgacgaca cccacgccca gccaggagac gatcgaccag
      361 ttcgtcacct tctgccacgg tcacctggcc ggtgtccagg agatgctggc cgccgatccg
      421 accttgatca acacccgctc gtcgctggac gaaacttgtc aggtgtcaag ctggccacgc
      481 cgatccacca cgctccgcag cacatgtcag tcctttctgc gattggaatg agatcggccc
      541 ctacgaggta gcacacccat cgcccaccac tcggtacact tcccaaccgt gaacgagcag
      601 gaccggcccc accaacctgg cggcatcgga cggcatctcc cggcgctggc gatctttgtt
      661 gccgctaccg tgctgctgac gtggccgctt gcctcgcgga tggacgatac cttcatctcc
      721 tggggcgatc ccgtcttcca gtcatggacg atcgcctggg attggcacgc gctcaagacc
      781 gacccgctcg ggatcttcga cgccaacgtc ttctatccct ggggtaacac gctggcctac
      841 tccgaccacc ttttcggcca ggcgctgacc gtcctgcctg tcatcgggct gaccgagaac
      901 ggcatcctgg ccgacaacat cgcgaccttg ctggcgttct tcttctcagc cctcgcgatg
      961 tatctgctcg tctacgacat gacgggcaac cggctggccg gcatcctggc cggcgtcgcc
     1021 tacgccttcg cgccatcccg cgtcgcccac ctggaacacc tgcacctgct ctcggcccag
     1081 tggccgccgt tgatgttgtt gtgtttgaga cgagctgttt gtcgggacgc gggacgcggg
     1141 acgcgggacg cggtcgaaga agatcgatct cccggtccgc cgtatccagc actcagtact
     1201 cagcactcgg ctcgtaatcc gcgtcccgcg tctcgcgtcc cgcgtcccgg tccgtggttc
     1261 ctcggcctcg gcgcctgctt cttcgcgcag gggttgttcg gcgtctactt cctgtacttc
     1321 tcgatcgtga tgctgatcat cgccggggcg gtctatgcgg ccttcgcgct ctatgaccgg
     1381 gactggcagg tcatgcgtgc gctggggctg gccgggctga cgtgtgcttt cgcgggggcg
     1441 ctactgattc caacgctgct gccctactgg caggtcaatc aggaactcga tgtcgagcgg
     1501 acaacggccg aggtgaacgc ctggagcgcc gagccgagcg actacctggc cgtctggccg
     1561 cgcgcgaagc tgtggaacga tctgctccag cgtaaccacc gcgatgtcga gcgggatctg
     1621 ttccccggcc tgttcattat cgcgctggcc ctgatcggcc tgacccaccg ggaggcaggc
     1681 cgccagcgct gggtgctgct ggccgttgcg gtcggtgggg tcgtcttctc gttcggtctt
     1741 tcagccacga ttgccggccg cgatgtcccg ctgccgtatc gaatcttcta cgactggatg
     1801 ccggggttcc gggcaattcg cgtcccggcc cggctggggc tgctggcgct ggtcggcgcc
     1861 ggcgggctgg ccgggctggg catcgaccgg ctctggcggc tcggccgcga cgagttgccg
     1921 ctcttccgct ggtatccgca cccgctggcg caacggccgc gcctggtcgg cagcgccgtc
     1981 gcgacgctgg cgctggtctt catcctgctc gaaacgctgc ccaggatgga gctgcccgac
     2041 ccactcccgg ccccgccgga caacccgccg ccggcctacg cctggattcg cgacaacccg
     2101 gcgccgacga tcgagctgcc aatgggcgag gggctggtcg gctcggcctg gccgaacttc
     2161 tggtcgatgt tccactggaa cgagaccgtc aacggctact cagggatcgt cccgccgagc
     2221 tactacccgt tccgcgagga gatgcgcgcc tttccgtccg agaagacgat ccggctgctg
     2281 caagggatcg gcgtcgagaa tgtcatcatc cactccgact tccccgaccg cgaccgcgcc
     2341 acgctggacg ccgaactcgc ggcccacccg gagctgacgc tggcactggc cggccccgac
     2401 gccgtctacc ggctggcgcc cgacccgtgg atgtgggatc tcgcggacgc catccccagc
     2461 gacgccatcg tcgaccttcc caacgccgga gccgacccgc ccacctacgg cctgctgatg
     2521 gcgatcctcc agcggaccga ccacgatgtc gccggccagg gtcagatcga ctactacacc
     2581 ttcaccccac ctgacagcac cacctgctac gcgatcctcg cggacagcga cgaccctgcc
     2641 gcgtacggct accccgccgc ccgcccagtg ctccaggaag gcgggctgac gctgtatgtc
     2701 cggcccgatt gcgggggata agcgatcggg agtaacggta caattgccga gacgcttgcc
     2761 gtaggtagaa agagaaccgt catggccgtt cagcgatccg ccgatcacga tcctgccgat
     2821 gacattcagg tcgttgaaat ctctcgcgaa gaaggcatcg cgatgttcga tcgacaggcg
     2881 cgcgagcggc tgggcctgag tggcgaagaa ttcctgcgga agtggaatgc cggcgagatc
     2941 gaggatccag atcgtaatga tgtgatgatg ctggtcatga tgatcccgtt cacaaagtag
     3001 gcccgatacg gtgtcggcac gccttcggcg agacagcatc catgcctatc acgccacgat
     3061 caagcggctc atcagctgtg tcgttcggac gcacatcacc acacgagcgc ctctgccaga
     3121 aatcgccaac gttgtgccgg atgccatggc gcttgtcctg cacaatggtc agcctgccca
     3181 gcttcacgga caagttccgc ttctcttgga tattgctcag catttccgta ccgtagcgat
     3241 tgacgctcca cgtagcctgc gcatcgaagt cgtcagctac gtgtacacgg taatgactaa
     3301 cgagcgaatt gaacatgtgt catagcaatg gcatccccac ggcgaaggtg acattgatat
     3361 tccacatgtc cacatcgggc cagcgatgtc gcgacatgac tcgattgtcc ggcccggcga
     3421 ggcacacaag atccacctgc ctaccggaga ggtttccctt gcgaatgttg tgcggctcac
     3481 gatcaccgaa tttggcgtcc agccgaggcg cgatgattgg caattgatcc tcacccagtc
     3541 cagccggccc tagccaaccg gcctcggtgg tggggtgccg tagcgccaga ggtagtagtg
     3601 ctggccgacg ttgccggctt cgacctccca gccggccggg ttgtcgggtg tccaggtcag
     3661 gcagcggcgc tcgaagcatt gcagcaggac gtccttcggt tgccctttca gcaggacacg
     3721 cacccagtag gcttcggtga tcgggtagcc ggtcgcgtag aacgggtctg ggaagagcgg
     3781 ctcggtctgg taacgctcat cgtcccagac ggtgccggtt ccggtcatga acgcccagaa
     3841 cggctcggcg acccagtggc cggtctccgg cgcgaaccag gtcacgccaa cgtcgtacgc
     3901 ggcgaaacgg gcgtcggtcc cgacgatgcc ggcccggtcg accgtccagg tcagcggtcc
     3961 cggttcgggg ccaggatacg gaacccgcag gttcgccatc gtcgcgtagg tgacgccgtt
     4021 cgggtcgtcg gcatcgccgg cgacgttgat gttggccggg ccgcggttct cgaactgtgc
     4081 gtcgccggtc tggagctggc cgctgaccag ctcgacgacc agcagcccgt tgctgacgaa
     4141 ccacgggtcg ctgcggtcgg cggatggatc ggtgatctcc atccgtgact tgtcgaagta
     4201 gatgaccttg cgctggccat ccggcgagtc ggcgtacgct tcccaggtca tgtgggccga
     4261 cgattgcggt ccccacatcc aggtccggtt gatgacacca tccatcaccg gcttatcagt
     4321 tcgctgccag ttctccagca gcgggtcacc atcctcctgg gcgacggcaa ccatgctgaa
     4381 tagcatcagc acgcagcacg cgagcgcggc agccactccc ggccgcccgc caacctgcct
     4441 tcccattgtc agctctcttc cgccccggaa tttcagctat cccatgttgg cattccgacc
     4501 ctgccgtgcc cggacacccc accaccgtga ctcggaggca ccgtcggatt tgtcagccta
     4561 tcacacggag gttgccggca ggcatgcgcc tgtcgagtgg aggggatgta tctacgccaa
     4621 cgctccgcgc cggatgcggc cgcttacgat ctgtcgccca ggatctcgct caactggccc
     4681 tggcattgct cgcgctcccg ttcacgttcg cgataccagg caagctcgtt ttcgagccgg
     4741 gtgatgcgct gatcgatgcc aaccagccag ttcgtaaaga gcgcggccgt gtcgccggtt
     4801 ggcgtcgtct ggatcggcgg gagcgccggt ggcgcaatgt caccaccact ccagatctcg
     4861 gtcagccgca acgcttcagc caggttaccg atctccgatg gagagagcgt cacaccggtg
     4921 cgcggtgtcc agccgcgaac atgccgcagc cagtcgtcgg tgttgaacca ccagcgcccg
     4981 taccggctgg gcggcgccag cggcagccgg tgggtcgttc ccggcggaga cagcagcagc
     5041 aggtccggcc agacgcggaa ctcggtggcg gggcggatgt cagccgtcga gcgatagagc
     5101 tgctggttgt tccagagcca gtcggccatc gcgttggcgg aattgaccgc ctgccagtag
     5161 gggttcgtgt ccgacccatt cgtcggtagc tcgacccagt caccgccgcc gttcagcttc
     5221 tcccagatac cgttgacggc gccgcgaatc ggcttcgcga tgcgcttcaa ctcggtcaga
     5281 atcagcgtcg atggcgcgtc gggccggatg acgaaccagt cgacgttgcg gccgatatcg
     5341 aattcagcga agatccagaa gctgtcaggc agctgcttga gcgtgtcgag aacctcctgg
     5401 tcgatcatgc tgaactcgcc ggcgaagatt cgtgccatgc agcgatccct ctccccaatt
     5461 gcgttacgac attcccccat tgcgatccgt gacgactgcc gcacgttatg tgaaatagcc
     5521 ctaacgggag caccggtgac atgtccccag tgcgacatcg gtgcgtcgga ttctcgttcg
     5581 gctatctatg cagacattac agcgtaatcg cgaatcaggc aaaccctatt cagagggtga
     5641 ggcgggcagg aacgcgaggg ccagccctgt tcgtctatca tgctcccgat tgcgatctgc
     5701 cgggctggca gcaatctggc ggagaccggc atgagattcg gtcttggcgc gttatcaacg
     5761 gcgacgataa agcgatctgg cacacggatg tgccggttga tgggaggaac ggtgctcaca
     5821 ctcggttaca aggcatcggc ggagcagttt ggcccgcgcg aattgctgga gttctcggtt
     5881 atcgccgagg aggcaggctt cgattccgtt gtcgtcagcg accacttcca gccctggcgg
     5941 catacgcacg gccactcgcc gttctcgttc gcctggctgg cagcgctcgg tgagcgaacc
     6001 agccgcgtcc ggctcggcac cagcgtcgtg acgccgacct tccgctacca cccctcgatc
     6061 gtcgcccagg caatggggac gctcggctgt ctcgcaccgg gacgggtctt cctcggtgtc
     6121 ggcaccggcg aatcgctcaa tgagatcccc tcgatcggga tcgagtggcc ggatttcaag
     6181 gagcgcttcg cccgcctgcg cgagtcgatt acgctgatgc agcggctctg gaccgaagac
     6241 cgcgtgacct tcgatggcac ctactaccag acgcgcaacg cgaccgtcta cgaccggcct
     6301 gaagagggca tcccgctcta catcgccgcc tcgggcgcga ccgccgcccg gctggccggt
     6361 cgagtcgccg acggcttcat ctgcaccagc ggcaaggcgc cggagctgta tagcgagacg
     6421 ctcgtcccgg ccgtccagga gggcgctggc aaggccggcc gcgacgcgag caagatcgag
     6481 atgacgatcg agatgaaggt ctcgtacgac catgacaagc agcgcgcgat ggaggacacc
     6541 aagcactggg cggcgttggc cctctccccc gacgagaaga ccggcgtcga ggacccgctg
     6601 gagatggagc gcctcgccga cgcgctgccg gtcgagcggg ccgccagccg ctggatcgtc
     6661 tccaatgacc cggacgagca ggtcgagaag atcaagacct acatcgatct cggcttcacc
     6721 cacctcatct tccacgcccc cggccccgac caggaacgct tcatccgcct gtatgggaaa
     6781 gaggtgcttc cgagattgcg cgcgctgtga acgacgggac gcgaggcgcg aggaatcggc
     6841 ggtccgcgtc tgacgtcccg ccgctacgcg tccctctcgt gctcccagcg atcgtcaccc
     6901 ctgtacaacg ctcggagatg gttgatctcc ccagcatggt agaggtcgtg ctggatcatc
     6961 acggcgatga tccagcgcgt ctcccgctgc tcgccccagt tcgtcaggcg acgttggtcg
     7021 aggtcgacgt cgtcgagtct ggagacacgg tagcgaaggc gttcgtgagc ctcacgcagc
     7081 caggcgagag ccgtcgcgac tgattcaagg gcgacgccgc cagtgacgac gggatcctcc
     7141 cagcccaggg atgcatcacc gaaggcgtag tcttcataca tgatcttgca gctaccgatg
     7201 tgttcgacca tgtcgcgcag cgaccggctt ccgcgtggtg gtgtccagca ccactcgtcg
     7261 tccgtgatat cgcgaagatt gccgaggagc gagtgccagt cgttgccggt gaacgcctca
     7321 tcgaggagat acaggagttg atcgacagcc accgccatcc tcgctcctca gtatcgttgg
     7381 cggatgatgt aacaacagcg gtggtcgcca ctcatcagat ggcaggagcg ttcgatgtcg
     7441 gcatcgagca ggcggcggta ggtttcgagc tcgtag
//
LOCUS       DASUGJ010000702         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_392108, whole genome shotgun sequence.
ACCESSION   DASUGJ010000702 DASUGJ010000000
VERSION     DASUGJ010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392108"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFV93_15945"
     CDS             <1..373
                     /locus_tag="VFV93_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5166898.1"
                     /translation="DAAERARNGAGPTLLETKTYRFAGHSKSDTGGKYRPKEEIAAWQ
                     ARDPLTSWRGFLLEHGVPDTLIAGLEAEIEQEVEAASQFALDSAYAEDEALSGAYAAT
                     SSSPAGRHAGTLAAAGIGGFA"
     gene            370..1341
                     /locus_tag="VFV93_15950"
     CDS             370..1341
                     /locus_tag="VFV93_15950"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5166899.1"
                     /translation="MTLAIATRTISFRDALYEALREEMERDETVILLGEDFANGGAFG
                     VAGDLPDCFGPERVLATPISENGFVGVAVGAAMTGLRPVVEIMFMDFIALAMDQITNH
                     ASKIHYMYAGQYNVPLVIRTPFGAGRGYGASHSQSLEGWLIQVPGLQVVAPSDPADAK
                     GLLQSAIREDNPVVVIENKLLYPKQGEIEDTGMPVPLGVARVRRNGGDITIASYGRML
                     DMALEAADLLATEGVGCEVIDLRTLKPLDLRTLADSVSRTGRFVFVEEGTGGVGAEAC
                     TRVVEACPGARVVRVAARDVPIPSSGPLEQHVIPQVTDIIQGCLNSL"
     gene            1376..2524
                     /locus_tag="VFV93_15955"
     CDS             1376..2524
                     /locus_tag="VFV93_15955"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5166900.1"
                     /translation="MASDGVASMEIPFVDLKAQYTTIREQIVTGFDEALAGMNLLLGP
                     NVQAFEVEFAEYCQARYALGVANGTDALYLAMKACGVGAGDEVITVSHSFIATAEAVV
                     NLGAVPVYVDVDADTYTLDPDKLKAAITSKTRAIVPVHLYGQMADMDPIMEIARQYGL
                     VVIEDACQAHGAEYRGRRAGSIGDAAAFSFYMGKNLGAYGDAGAITTNSKAVADHIRL
                     LRDHGSRVKYEHDEMGINSRLDELQAVVLRVKLPYLDGWNAARREHANTYRRLLEEAY
                     VTLPVEAGWNKHNYHLFVIQADERDHIRDILAKQGIATGIHYPIPIHDQIASRSIGRV
                     AGSMQVTDRLAPRILSLPMYAELELAQQEFVAVALRSATTAARAIIAD"
     gene            2536..>3183
                     /locus_tag="VFV93_15960"
     CDS             2536..>3183
                     /locus_tag="VFV93_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166901.1"
                     /translation="MYQQPSTGYANAGEPQEHDPLMQFAKRWWWLLVAGAIVGIGLAI
                     VYSKVGPVPYESTSLIQVVPPSGATTTEKADQAQTASANFAAEVSSPRIYTLTSQALA
                     GEIQITTSDLEAMERDGSLTVSPMRGSNFIKVVVTDSDRGRAKLIAGTLASVFVDDIT
                     QRASAANAARQSQVNQQIEFTRQQLATSALRQREIDLTDELRDRQSALLDLQTNYQ"
BASE COUNT          581 a         1060 c         1055 g          487 t
ORIGIN      
        1 agacgcagcc gagcgcgctc gcaacggcgc ggggccgacg ctgctggaga cgaagaccta
       61 ccgcttcgcc ggccactcca agagcgacac cggcgggaag taccggccca aagaggagat
      121 cgccgcctgg caggcgcgcg acccgctcac cagttggcgc gggttcctgc tggagcatgg
      181 cgttcccgac acgctgattg ccgggctaga ggccgagatc gagcaggagg tcgaagcagc
      241 cagccagttc gcgctcgaca gcgcctatgc cgaggacgag gcgctctccg gcgcctacgc
      301 cgcgacatcg tcgtcgccgg ccggacggca cgcgggcacg ctggcggcag ccgggatcgg
      361 agggttcgca tgacactggc gatcgcgacc cggacgatca gtttccgcga cgcgctctac
      421 gaagccctgc gtgaggagat ggagcgcgac gagacggtca tcctgctcgg cgaggatttc
      481 gccaacggcg gcgcgttcgg tgtcgctggc gacctgccgg attgcttcgg ccctgagcgg
      541 gtgctggcga caccgatctc cgagaacggc ttcgtcggcg tcgccgtcgg cgcggcgatg
      601 accgggctcc ggccggtcgt cgaaatcatg ttcatggact tcatcgcgct ggcgatggac
      661 cagatcacca accacgcctc gaaaatccac tacatgtacg ccggacagta caacgtcccg
      721 ctcgtgatcc gaacaccgtt cggagccggc cggggctacg gggccagcca ctcgcagagc
      781 cttgaaggct ggctgatcca ggtgccgggg ctccaggtcg tcgcgccgag cgaccctgcc
      841 gacgccaagg ggctgctgca gagcgccatc cgcgaggata accccgtcgt cgtcatcgag
      901 aacaagctgc tctatcccaa gcagggtgag atcgaggaca ctggaatgcc cgtcccgctc
      961 ggcgtcgcac gggtccgtcg caacgggggc gacatcacga tcgcgtcgta tggccggatg
     1021 ctcgacatgg cgctcgaagc ggccgacctg ctggcgactg aaggcgtcgg ctgcgaggtg
     1081 atcgacctgc ggacgctcaa gccgctcgac ctgcggacgc tggctgactc ggtctcgcgg
     1141 accgggcgct tcgtcttcgt cgaagagggc accggcggcg tcggcgccga ggcctgcacc
     1201 cgcgttgtcg aagcctgccc cggcgcacgg gtcgtccgcg tcgccgcccg cgatgttccg
     1261 atcccatcct ccgggccgtt ggaacagcac gtcatccccc aggttaccga catcatccag
     1321 gggtgtctca acagtctgta gagggcatcg tgcccgcacg tggaaggaca gcggcgtggc
     1381 atcggacgga gtggccagca tggagatccc gttcgtcgat ctgaaggcac agtacacaac
     1441 gatccgcgag cagatcgtga ccggctttga cgaagcactg gccgggatga acctgttact
     1501 tggtccgaac gtgcaggcgt ttgaggtgga gttcgccgag tactgccagg cgcgctatgc
     1561 gttgggcgtc gccaacggca ccgatgcact ctacctggcg atgaaagcct gcggtgtcgg
     1621 cgccggtgac gaggtcatca ccgtctcgca cagcttcatc gcgacggccg aggcggtcgt
     1681 caatctcggc gccgtcccgg tctacgtcga tgtcgacgct gacacctaca cgctcgatcc
     1741 ggacaagctg aaggcggcga tcacctcgaa gacgcgggca atcgtcccgg tccacctgta
     1801 cgggcagatg gccgacatgg acccgatcat ggagatcgcc cgccagtacg ggctggtcgt
     1861 gatcgaggat gcctgccagg cgcacggggc cgagtaccgg ggccggcggg ccggcagcat
     1921 cggcgatgcc gccgcgttca gcttctacat gggcaagaac ctcggcgcct acggcgacgc
     1981 cggggcgatc acgaccaact cgaaggccgt cgccgaccat atccggctgc tgcgagacca
     2041 cggctcgcgg gtcaagtacg agcacgacga gatgggcatc aactcacgcc tcgacgagct
     2101 ccaggccgtc gtcctgcgcg tcaagctgcc ctatctcgac ggctggaacg ccgcccgtcg
     2161 ggagcacgcc aacacctacc ggcggctgct tgaagaagcg tatgtgacgc tgccggtcga
     2221 agcgggctgg aacaagcaca actatcacct gttcgtcatc caggccgacg aacgcgacca
     2281 cattcgcgac atccttgcca agcagggaat cgcgaccggc atccactatc cgatcccgat
     2341 tcacgaccag atcgcctcgc ggtcgatcgg ccgggtcgcc ggcagcatgc aggtcaccga
     2401 ccggctcgcg ccgcgcatcc tgtcgcttcc gatgtacgcc gaattggaac tcgcccagca
     2461 ggagttcgtg gccgtcgcgc tgcgcagcgc gacgacggcg gcccgcgcca ttatcgcgga
     2521 ttgatggaga gcgtgatgta tcaacagccg tcgacaggat atgcgaatgc gggggagcca
     2581 caggagcacg acccgctgat gcagttcgcc aaacgctggt ggtggttgct ggtggccggc
     2641 gcgatcgtcg ggatcggact ggcgatcgtc tactcgaagg tcgggccggt cccgtacgag
     2701 agcacgtcac tgatccaggt cgtgcctcca tccggagcaa caacgaccga gaaggccgac
     2761 caggcgcaga ccgccagtgc caacttcgcg gccgaggtgt cgtcaccaag gatctacacg
     2821 ctcacgagtc aggcgctggc gggcgagatc cagatcacga ccagcgatct ggaagcgatg
     2881 gagcgcgatg gctcgctgac ggtctcgccg atgcgcggct cgaacttcat caaggtcgtc
     2941 gtgacagatt ctgaccgtgg tcgtgccaag ctgatcgccg gcacgctcgc cagtgtcttc
     3001 gtcgacgaca tcacccaacg cgcgtcggcc gccaacgccg cgcgccagtc acaggtgaac
     3061 cagcagatcg agttcacccg ccagcagctg gcaacgtccg ccctgcgcca gcgggaaatc
     3121 gacctcaccg acgagctgcg tgatcggcag tcggcgttgc tggacctgca gaccaactac
     3181 cag
//
LOCUS       DASUGJ010000703         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2059946, whole genome shotgun sequence.
ACCESSION   DASUGJ010000703 DASUGJ010000000
VERSION     DASUGJ010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2059946"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="VFV93_15965"
     CDS             <1..1566
                     /locus_tag="VFV93_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166902.1"
                     /translation="NAQVVAGVCRRVDGLPLAIELVAARVRLLPPAALLDRLERRLPL
                     LTGAPNDAPPRQQTLRGTIAWSYDLLNPATQRLFRALGVFRDGWTLDALESVSGQTDA
                     GSVVLDGLETLIAHSLVRMHEQADGQPRYSMLETIAEFAREKLDASDEALEARRRHVH
                     HYLRLARKAEPHLKGAEQARWMATLEQEFANLLGALRWLREQAKQGDAAAVVTSFQIA
                     DPIWWFLHIHGHMREGRQQFDTIMRLLQEQQAAVTALTGPAEWSRMRARTLFYVAALD
                     TWQADHHDEHAWRMMQESLAIYRRLDQRADIASTLMFLGYGAQRIGNFAEAEGYLMEG
                     LTVARDAGDDGVAALLSQGLGMIGLRRGDYERGSGWVKESLALFTCLGDERGIAASRA
                     TLGALLLRQDDLAQANALLRESLTARHQVGDKGGIAWCLEWLAEAAMLGGSPQDSLLR
                     AARLLGAATALRAAIDSPIDPVDMPEHERVMASVQAQLSDPAFVAAWEAGRAMALDDV
                     VGYALATPSFINR"
     gene            complement(1549..1977)
                     /locus_tag="VFV93_15970"
     CDS             complement(1549..1977)
                     /locus_tag="VFV93_15970"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5166903.1"
                     /translation="MSTQANKELVLRAYKELVNEGSYDNLEELCHDDYYDNTQPPGWP
                     TDRAGLQRQLLYFRSAFPDINVTFEDIIAEGDIVMLRQTMRGTHLGEFFGIPPTGKQV
                     AMTGSHLFRIKEGKLVEHQANNDDLGMLRQLGVIPSPVDE"
     gene            2265..>2746
                     /locus_tag="VFV93_15975"
     CDS             2265..>2746
                     /locus_tag="VFV93_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166904.1"
                     /translation="MHPLRLFAATGEQVVRLDSHDGERFETTVMLPESKAQCVAVDPS
                     NPDRVYVGTFDDGVYRTLDGGETWTHVSEGIPHKRVLSIAISPSDRVNGLNAVFAGTE
                     PSNLYRSEDDGATWQEFSELPKLPSSPGWSFPPRPWTSHVRWIAPHHADPNLLYVGIE
                     L"
BASE COUNT          483 a          873 c          887 g          503 t
ORIGIN      
        1 aacgctcagg tcgtcgccgg cgtctgcagg cgagtagatg gcttgccgct ggcgatcgag
       61 ctggtggcgg ctcgtgtccg gctgctccca ccggcggcgc tcctcgatcg cctcgaacga
      121 cggttgccgc tgctgaccgg cgcgcccaac gacgccccgc cccggcaaca gaccctccgc
      181 ggtacgatcg cctggagcta tgacctgctg aacccggcaa cgcagcgcct gtttcgcgcg
      241 cttggcgtct tccgggatgg ttggacactc gacgcactcg aatccgtctc gggacagacc
      301 gacgcaggct cagtcgtgct ggacggactc gaaacgctga ttgcccacag cctcgtgcgc
      361 atgcacgaac aggcggatgg ccagccacgc tattcgatgc tggagacgat cgccgagttc
      421 gcgcgcgaga agctcgacgc gagcgacgag gcgctggagg cgcgtcggcg acatgtccat
      481 cactacttgc ggctcgcgcg gaaggccgag ccacacttga aaggcgccga acaggcgcgc
      541 tggatggcaa cgctggaaca ggagttcgcc aacctgcttg gcgcgcttcg atggctgcga
      601 gaacaagcga agcagggtga cgcggcagcg gtcgttacca gctttcagat tgctgatcct
      661 atctggtggt tcctgcacat tcacggacac atgcgtgaag gccggcaaca gttcgacacc
      721 atcatgagac tgcttcaaga gcaacaggcg gcggtcacgg ctttgactgg cccggcggag
      781 tggtcacgca tgcgggcgag aacgctcttc tatgttgccg cgcttgacac ctggcaggca
      841 gaccaccatg acgagcacgc ctggcgcatg atgcaggaga gcctcgctat ctaccggcga
      901 ctggatcagc gggccgatat cgcctcgacc ctcatgttcc tgggctacgg ggcgcaacgc
      961 atcggcaact tcgctgaggc cgaaggctac ctgatggagg gtctcaccgt cgcccgcgac
     1021 gcgggcgacg acggcgtcgc tgcgctcctg tcgcaggggt tggggatgat cgggctacgt
     1081 cgtggcgact atgaacgcgg tagcggctgg gtaaaggaga gtctggcact gtttacctgc
     1141 ctcggcgacg agcgcgggat tgccgcctca cgcgcgacgc tcggcgcgct cctgctgcgt
     1201 caggacgatc tcgcgcaggc caacgcgctc cttcgtgaga gcttgacggc acgccatcag
     1261 gtcggcgaca agggcgggat cgcctggtgc ctggagtggt tggccgaagc ggcaatgctc
     1321 ggcggttcac cgcaggacag tctcctgcgg gcggcccggc tgcttggggc agcgacggcg
     1381 ctgcgggccg cgatcgactc tccaatcgac ccagtcgata tgccggagca cgagcgtgtc
     1441 atggcgagcg tccaggccca actgagcgat ccggccttcg tggcggcctg ggaagccggg
     1501 cgagcgatgg cgctcgatga cgtggtcggc tatgcgctcg cgaccccgtc attcatcaac
     1561 aggtgatgga atcacgccga gctgtcgcaa catccccagg tcgtcgttgt tcgcctgatg
     1621 ctcaaccagc ttaccttctt tgatcctgaa cagatgtgat cccgtcattg cgacctgctt
     1681 accagttggt ggaatgccaa agaactcgcc caggtgggta ccgcgcatcg tctgccgcag
     1741 catcacgatg tcgccctcgg cgatgatgtc ctcgaacgtg acgttgatgt cggggaaggc
     1801 gctgcggaag tagagcaact gtcgctgaag accggcccgg tcggtcggcc agcccggcgg
     1861 ctgggtatta tcgtagtagt cgtcgtggca gagctcctcc agattatcgt agctgccctc
     1921 attcaccaac tctttgtagg ctcgcaggac gagttccttg ttggcttgcg tggacatcgc
     1981 gatctccttc attcatctgc ggctactcga cggctggccg gtctgcgccg cctcgccctg
     2041 atcgcagtat cttctgaagg gtccgcgaac tccatgacga atccacaggc tgacccacag
     2101 aaatgtgatc ggctggatct gaggaacgcc gatcggccgt cccggttggc gctcggccac
     2161 cccgtcggat ggctacgaca ttcttccgat tcgcgggaat cggcttcgtg ctacaacacc
     2221 gccagagcag acgttctcga cgagcgcaat ccagggaggc gcacatgcat ccacttcgac
     2281 tctttgcagc caccggcgaa caggttgtcc ggctcgatag tcacgatgga gaacggttcg
     2341 aaacgaccgt catgctgcct gagagcaagg cgcagtgcgt tgccgtcgat ccatccaatc
     2401 cggaccgtgt ctacgtcggg acgttcgacg acggggtcta ccggacgctg gatggcggtg
     2461 agacgtggac gcacgtgagc gagggtattc cgcacaagcg cgtcctctcg atcgccatct
     2521 cgccgtcaga ccgggtgaac ggcctcaacg ccgtctttgc cgggaccgag ccgagcaacc
     2581 tctaccgctc tgaggacgat ggcgcgacct ggcaggagtt ctccgagctg ccgaagctgc
     2641 cgagctcgcc aggttggtcg ttcccgcccc gcccatggac gagtcatgtc cgctggatcg
     2701 cgccgcacca tgccgacccg aacctgctgt atgtcggcat cgagct
//
LOCUS       DASUGJ010000704         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1503123, whole genome shotgun sequence.
ACCESSION   DASUGJ010000704 DASUGJ010000000
VERSION     DASUGJ010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503123"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1099
                     /locus_tag="VFV93_15980"
     CDS             191..1099
                     /locus_tag="VFV93_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5166905.1"
                     /translation="MPQQRTEYLSTRRIGNAEVTLILEGSGPYPVELNVAEGDWRPAV
                     PEADADGNVMLCSGGAYVRIGDASVVIDPGLDDPGTATSAATELVFRGWSFTPGFQAA
                     LDELGVDNDAVTHVIITHAHFDHCLGVAVERDGRLVPRYPNARYLLDRADWDLHFTPE
                     GEQRPLAASPFIEVNRALRDRLQVIMRTGLFDLLDGEAEVVPGLTTIPTPGETPGHRA
                     VRIESNGAICWHLGDIAHYAVEFEHLDWLTPTRRDAAAMTASRQRILPRIVAEDALVT
                     WSHARFPGWGRIVAVDGGYRWQPEFS"
     gene            complement(1123..1584)
                     /locus_tag="VFV93_15985"
     CDS             complement(1123..1584)
                     /locus_tag="VFV93_15985"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX5166906.1"
                     /translation="MDDTTAMERCIELARAAAARGDQPYGSVVVLDGRIVAEADNSIA
                     TDLDPTAHAELVAIRRACQALGRTDLAGATIYASGEPCWMCSTLIRQARLSRVVFAMP
                     SSSGTGGATSAFNILRAEDVAGLVPPPEVVGGFLEERVRALHAQLGWPPRR"
     gene            1757..2788
                     /locus_tag="VFV93_15990"
     CDS             1757..2788
                     /locus_tag="VFV93_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX5166907.1"
                     /translation="MPTPPEGQISKSDAYYHVVLERIPPRAEPPFEDAAMQERVWGRQ
                     WGVGDDVGRLRLVALHRPGDEMRTIDPDKYDETIEALIDDREQWYWRDSTGPDIDLMQ
                     RQHDALRAALEAEGVEIVEVGGSDGDVKAVFTRDQAIAVDGGAVICRMGPVGADPGYG
                     RRGEEGYITKVIANLGMPILRTIHGTGLIEGGSFCFLKPGVAALGMSFRQNEEGARQV
                     EEVLRATGTRLIRIPLTGHSLHLDGCILMVSHDQALINVTRLPYWFLDELTALGIEPI
                     HCWPNEGHAVNCLAIRPGKVLISEGCPWTVERLNQRGIETVELDYSEIRKNGGGIHCS
                     TLPLIRDRM"
BASE COUNT          554 a         1041 c         1011 g          489 t
ORIGIN      
        1 atagcagcaa cctctcccag attcgattgt gcgcctcacg cgatcgatac acccagcata
       61 cgccccgcga gcaccgacgt atagagggag atcgtcaccg gcaggcagtg acgatgccgg
      121 ttggcattca gtgtcatcac ccatgcgata ctgcgggcca gatccacatc gacgggaaga
      181 ggagagagcc gtgcctcaac aacgaaccga gtacctttca acgcggcgga tcggtaacgc
      241 cgaggtgacg ctgattctcg aaggttccgg gccgtatccg gttgagctga acgtcgctga
      301 aggcgactgg cgaccggctg ttcccgaggc agatgcggac gggaacgtca tgctctgcag
      361 tggcggcgcg tacgtccgaa tcggcgacgc ctcggtcgtc atcgacccgg ggctggacga
      421 tcccggcacg gcaacctcgg ccgccactga gctggtcttc aggggctggt cgttcacacc
      481 ggggttccag gcggcgctgg acgagctcgg ggtcgacaac gacgctgtca cccacgtcat
      541 catcacacat gcccatttcg accactgtct cggcgttgcc gtcgagcgag acggcaggct
      601 ggtgccgcgc tacccgaacg cccgctacct gctcgaccga gccgattggg atctgcattt
      661 cacacccgaa ggtgagcagc gcccgctggc cgccagcccg ttcatcgagg tgaaccgcgc
      721 actgcgcgac cgcctgcagg tgatcatgcg gacgggactc ttcgatctac tcgacggcga
      781 ggccgaggtc gttcccggcc tgacgacgat cccgacgccc ggcgagacgc ccggccaccg
      841 cgccgtccgg atcgagtcga acggcgcgat ctgctggcat ctcggcgaca tcgcccacta
      901 cgccgtcgag ttcgagcatc tcgactggct cacgcccacc cgccgcgacg ccgccgcgat
      961 gaccgcctca cgccaacgca tcctgccccg gatcgttgcc gaggacgcgc tcgtcacctg
     1021 gtcgcacgcc cgcttcccag gctggggcag gatcgtcgct gtcgatggcg gctaccgctg
     1081 gcagccggag ttcagctgat cgtgttcccc aaacgagcct ggctaccggc gcggcggcca
     1141 cccgagttgt gcgtgtagcg ccctgacacg ctcttcgagg aatccaccga cgacctcggg
     1201 cggtgggacg agaccggcga catcctctgc ccgcaggatg ttgaacgccg aggtcgcgcc
     1261 gccggtgcct gagcttgacg gcatcgcgaa gacgacgcgg gacaggcgcg cctggcgaat
     1321 cagcgtcgag cacatccagc acggctcgcc gctcgcgtag atcgtcgcgc cggccagatc
     1381 agttcgccca agcgcctgac aggccctgcg gatggcaaca agctcggcgt gggcagtcgg
     1441 gtcgaggtcg gtcgcgatgc tgttgtctgc ctcggcgacg atccgaccgt ccagcacgac
     1501 caccgaaccg tacggctggt ccccgcgcgc ggctgctgcc cgcgccaatt cgatgcaacg
     1561 ctccatcgcc gtcgtgtcat ccacggttca ggctcctccc ggatatccgc gccatcacga
     1621 gtcaggcaac cataccatcg gttccccgcg actccagcgc ccactggaca ggttctgcct
     1681 gttggacgac gatgttgact gccgaacacc ggcaacgacg agccaaacaa tcgagacctg
     1741 gaaatggagc ggcgagatgc caacaccacc agaggggcag atcagcaaga gcgacgcgta
     1801 ttaccacgtc gtgctggagc gcattccacc acgggccgag ccgccgttcg aggacgccgc
     1861 catgcaggag cgcgtctggg ggcggcagtg gggcgtcggc gacgatgtcg gccgcctgcg
     1921 actggtggcg ctgcaccggc ccggcgacga gatgcggacg atcgaccccg acaagtacga
     1981 tgagacgatc gaggcgctga tcgacgaccg cgagcagtgg tactggcgcg actcgaccgg
     2041 accggacatc gacctgatgc agcggcagca cgacgcgctg cgggcggcgc tggaagccga
     2101 aggcgtcgag atcgtcgagg tcgggggctc cgatggcgat gtcaaggccg tcttcacccg
     2161 cgaccaggcg atcgccgtcg atggtggagc cgtcatctgc cggatggggc cggtcggcgc
     2221 cgatcccggc tacgggcggc gtggcgagga aggctacatc acgaaggtga tcgccaacct
     2281 cgggatgccg atcctgcgga cgatccacgg cactggcctg atcgagggcg gctcgttctg
     2341 cttcctgaag ccgggcgtgg ccgcgctggg catgtcgttc cgccagaacg aggagggcgc
     2401 ccgccaggtg gaagaggtcc tgcgggcgac cggcacccgg ctgatccgca tcccgctgac
     2461 cggccactcg ctccacctcg acggctgcat cctgatggtg agccacgacc aggccctcat
     2521 caacgtcacc cgcctgccct actggttcct cgacgagctg actgcgctcg ggatcgagcc
     2581 gatccactgc tggccgaacg agggtcacgc cgtcaactgc ctcgcgatcc ggccggggaa
     2641 ggtgctgatc tccgagggct gtccctggac cgtcgagcgg ctcaaccagc gcggcatcga
     2701 aacggttgaa ctcgactaca gcgagattcg caagaatggc ggcgggattc attgcagcac
     2761 gttgccgctc attcgcgacc ggatgtaacc cggactgtca ccaaaaagac gccaacgatc
     2821 gttcccagga accgccagaa ggagggggcg gtcttgtacc ggcctgttcc ctaccaactc
     2881 ggccggagtg gaaacagggt cggcccctcc atgtgcaggt cgtagcccag gtgtctttcg
     2941 ttatgcaagg tggtattcct tgctatactt tcgagactgc gcgggcaaaa cctcgtgctg
     3001 gtggcggagc ctgtttcctt cggagtcggt caccagatca gcgcgtgcag acattgtcgg
     3061 gtcgcagaca ccccgcggga gcaaggacgg ccatg
//
LOCUS       DASUGJ010000705         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_809668, whole genome shotgun sequence.
ACCESSION   DASUGJ010000705 DASUGJ010000000
VERSION     DASUGJ010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809668"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VFV93_15995"
     CDS             <1..1004
                     /locus_tag="VFV93_15995"
                     /inference="COORDINATES: protein motif:HMM:NF018829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HEX5166908.1"
                     /translation="EYPHVDGYERGYEAVELAAKTVRGEVSPVMHSTVLPMMIAPITS
                     FHGPAREINELCWAWEAKPGIIDVAFIHGYPHSDVPVVSTSVMATADGDRELARQATE
                     AVARRIWEMRDQFLSQLPQPEEAIRQALAVEGGPVVIAEVSDNPGGGSPGDGTHLLRA
                     LLDARLAGSAFGFIYDPETAEQAHAAGPGATIRVRLGGKTDPPILGAPVEADAYVKSV
                     TDGQFITQSPMGRGGQRNLGKMARLVVDTVDVIVGSESSQTIDAELFLLHGIDISRYK
                     IIALKSQNHFRAAFEPLAAAIIRTDPPGWTTSNLNQLEFERVRRPIWPLNRDVEYDG"
     gene            complement(1026..1418)
                     /locus_tag="VFV93_16000"
     CDS             complement(1026..1418)
                     /locus_tag="VFV93_16000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5166909.1"
                     /translation="MSNARPRILVVDDDRPIRDVISTVLKEAGYDILQAIHGGEAIAL
                     VDDAPPDLIISDLMMPIVDGIELCRYAKARGDIAVILMSSAPPTVVDADHDAFLPKPF
                     ALDTLESLVAGVLARHDDPTTGPAPQPT"
     gene            complement(1411..2874)
                     /locus_tag="VFV93_16005"
     CDS             complement(1411..2874)
                     /locus_tag="VFV93_16005"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX5166910.1"
                     /translation="MVAVDQGLLSTGVPGLDLVLGGGIHQGSIVLFVGVPGSGKTILA
                     QQIAHHRASQGDRVLFLIGFSETQDKLIAHGRSMDFFVGSHLGREIQFGNLADLLRAG
                     IEETEDTIVAMARQQRARLVILDGFSSMRRSLPDEPTPGALLYSLGNKLSLLGATTIV
                     TTEGDLRRIDYEPEYTVADAIIGLGRMASGFRDRRVLDVNKVRGAAPLLGRHPFTIDT
                     RGITIYPRLETVARRRLDAWSPEVVPFGIPGLDTILGGGITAGSSTVVAGSPGTGKTL
                     LSLHFLAEGARRNEPSLLVEFIEDVVQIRAQARAFGLDGPIAGDGSVVELLGLPAYEM
                     EPDEIAHQICERLDTNGVRRLVIDSANELARAIDPIRQADFFAALIDILRSRGVTSLF
                     TFDVPSIVGTDLTFANSPLAGLAENLLLLRQLERHGSPQRALAVLKRRFGAADHVFYP
                     YSIQVPIGFEVVTIPAFSDDRSAALRADLCPLGANGE"
     gene            complement(3065..>3371)
                     /locus_tag="VFV93_16010"
     CDS             complement(3065..>3371)
                     /locus_tag="VFV93_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166911.1"
                     /translation="GPVALPAGDDDGDDALDFSLAREVGVLRLLLDRLEVLILTEADP
                     ERLVLPLTRLVESIARTIRIHSGVTGGPEDDLAGFIARAFDTLNLPGNLPSGRDDRN"
BASE COUNT          578 a         1117 c         1178 g          498 t
ORIGIN      
        1 atgagtaccc gcacgtcgat ggctacgagc ggggctacga ggcggtcgag ctggcggcga
       61 agacggtccg gggcgaggtg tcgccggtga tgcactcgac cgtcctgccg atgatgatcg
      121 cgccgatcac cagcttccat gggccggccc gcgagatcaa cgagctgtgc tgggcgtggg
      181 aggccaagcc aggcatcatc gacgtcgcct tcatccacgg ctacccgcac tcggacgtgc
      241 cggtcgtctc aaccagcgtg atggcgacgg ctgatggcga ccgcgagctg gcccggcagg
      301 cgaccgaggc ggtcgcgcgg cggatctggg agatgcgcga tcagttcctc tcgcagttgc
      361 cacagccgga ggaggcgatc cgccaggcac tcgctgtcga gggcggtccg gtcgtgatcg
      421 ctgaggtgtc ggacaaccca ggcggagggt cgccgggcga cggcacccac ctgctgcggg
      481 cgctgctgga cgcgcggctg gcgggctcgg ccttcggctt catctacgac ccggagacgg
      541 ccgagcaggc ccacgcagcc gggccgggcg cgacgatccg cgtcaggctc ggcgggaaga
      601 ccgacccgcc aatcctcggc gcgccggtcg aagccgacgc ctacgtgaag tcggtgacgg
      661 acggtcagtt catcacgcag tcgccgatgg ggcggggcgg tcagcgcaac ctgggcaaga
      721 tggcgcggct ggtggtcgac acggtcgatg tgatcgtcgg ctccgagagc agccagacga
      781 tcgacgccga gctgttcctg ctgcatggga tcgacatatc acgctacaag atcatcgcgc
      841 tcaagtcgca gaaccacttc cgggctgcgt ttgagccgct ggcggccgcg atcatccgca
      901 ccgacccacc cggctggacg acctccaacc tcaaccagct cgaattcgaa cgcgtccgcc
      961 gtccgatctg gcccctgaac cgggatgtgg agtacgacgg gtagacgatg gccggccccg
     1021 cagcgtcagg tcggctgggg tgcggggccg gttgtgggat cgtcgtggcg ggcgaggacg
     1081 ccggcgacca gcgactccag cgtgtcgagc gcgaacggct tcggcaggaa ggcgtcgtga
     1141 tcggcgtcga cgacggtggg tggcgccgag ctcatcagga tcacggcgat gtcgccacgc
     1201 gccttggcgt agcggcagag ttcgatgcca tcgacgatcg gcatcatcag gtcgctgatg
     1261 atcaggtcgg gcggcgcatc atcgaccagc gcgatcgcct cgccgccgtg gatcgcctgc
     1321 aggatgtcgt aaccggcttc cttcagcacc gtcgagatca cgtcgcgaat tgggcggtcg
     1381 tcgtcgacga ccagaatccg tggccgtgca ttactcaccg ttggccccta gcggacacag
     1441 gtccgccctc agtgcggccg accgatcgtc ggagaatgcc ggtatcgtca ccacctcgaa
     1501 cccgatcggc acctgaatcg aatacggata gaagacgtga tcagcggccc cgaagcgccg
     1561 cttcaggacg gccagcgcgc gttgcggcga tccgtgccgc tcgagctgcc gcagcaacag
     1621 caggttctcg gccaggccgg ccagtggtga gttggcgaag gtgaggtcgg tcccgacgat
     1681 ggacgggacg tcgaacgtga acaggctggt gacgccgcgc gagcggagga tgtcgatcag
     1741 cgcggcgaag aagtcggcct gccggatcgg atcgatcgcc cgcgccagct cgttggccga
     1801 gtcgatgacg agccggcgga cgccgttggt gtcgagccgt tcgcagatct ggtgggcgat
     1861 ctcgtccggc tccatctcat aggcaggcag ccccagcagc tcgacgacgc tcccgtcgcc
     1921 cgcgatcggg ccgtccagcc cgaaggcacg cgcctgggcg cggatctgaa cgacatcctc
     1981 gatgaactcg accagcagcg acggctcgtt gcgccgcgcc ccttcggcga ggaaatggag
     2041 cgagaggagc gtcttgcccg tcccggggct gccggcgacg acggtgctgg acccggccgt
     2101 gatgccgcca ccgaggatcg tatccagccc aggaatgccg aacgggacga cctccggcga
     2161 ccaggcatcc agccggcgac gggcgaccgt ctccagccgt gggtagatcg tgatgccgcg
     2221 ggtgtcaatc gtgaacgggt ggcggccgag cagcggggcc gcgccgcgca ccttgttgac
     2281 gtccagcaca cgccggtcgc ggaagccgga cgccatccgc ccgaggccga tgatcgcgtc
     2341 ggcgacagtg tactccggct cgtagtcgat gcgcctgagg tcgccctcgg tcgtgacgat
     2401 cgtcgtcgcg cccagcagcg agagcttgtt gccgagcgag tagagcaacg caccgggagt
     2461 cggctcgtcg ggcaggctcc ggcgcatgct ggagaagccg tcgaggatga ccagccgggc
     2521 acgctgctgg cgagccatcg cgacgatcgt gtcctcggtc tcctcgatcc cggcccgcag
     2581 gaggtcggcc aggttgccga actggatctc gcggccgagg tgggagccga cgaagaagtc
     2641 catgctgcgt ccatgcgcga tgagcttgtc ctgtgtctcg gagaagccga tcaggaagag
     2701 gacgcggtcc ccctggctgg cgcgatgatg ggcgatctgc tgggcgagga tcgtcttccc
     2761 gctacccggt accccaacaa agaggacgat tgatccctga tgaatcccgc caccgaggac
     2821 gaggtcgagc cccggcaccc cggtcgacaa tagcccctga tcaacagcga ccaccgcttc
     2881 gtccaaccct tcgctacccc acaactgtgc cgccgtgcac catactgcca gccgctctgt
     2941 agggcaggaa aacgcacgga acatgccaag attctgccgc ccggaggcag cccaccgcac
     3001 cggcacagct acgccgtcac gatcataacg acgatgcacg tccaccgtcg gacctgggcc
     3061 tcgatcagtt tcgatcgtcc cgaccggatg gcaggttgcc gggcaggttg agggtgtcga
     3121 aggcgcgggc gatgaacccg gccaggtcgt cctcggggcc gccggtgacg ccgctgtgga
     3181 tgcggatcgt gcgggcgatc gactcgacca gccgggtgag gggcaagacc agccgctcgg
     3241 ggtcggcctc ggtcaggatc agcacctcca gccggtccag cagcagccgc aggacgccaa
     3301 cctcccgtgc cagcgagaaa tccaatgcgt cgtcaccatc gtcatcaccg gccggcagcg
     3361 caaccgggcc g
//
LOCUS       DASUGJ010000706         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_256556, whole genome shotgun sequence.
ACCESSION   DASUGJ010000706 DASUGJ010000000
VERSION     DASUGJ010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256556"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFV93_16015"
     CDS             complement(<1..467)
                     /locus_tag="VFV93_16015"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEX5166912.1"
                     /translation="MAAAGDAIPLEDQIGQWRNYLRRRQAIHAVDVAELEDHLREQVA
                     VLGEAGLDSDEAFLVAVKRMGNLDALSREFAREHSERLWKQLVVAPAESGETRTWVRT
                     DAVVAFCLAIVAAFLIKIPALFGMQIDEDASFYARNASLFVLPLLTGYFVWKR"
     gene            complement(470..847)
                     /locus_tag="VFV93_16020"
     CDS             complement(470..847)
                     /locus_tag="VFV93_16020"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5166913.1"
                     /translation="MEINKDLIAASSTPIVLAILAEADSYGYAILQRVRELSGGQMEW
                     TDGMLYPVLHRLERLGHVAARWETAESGRRRKYYRITGQGRSQLAEDRRQWQAVDATL
                     RGIWQVLSFPAPAGQPATQPQGA"
     gene            988..2589
                     /locus_tag="VFV93_16025"
     CDS             988..2589
                     /locus_tag="VFV93_16025"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5166914.1"
                     /translation="MASEHRWWQTGIVYQIYPRSFQDSNGDGIGDLAGITSRLDYVRS
                     LGVDAIWISPIYPSPMADFGYDVSEYIGIEPVFGTLDDFDRLVAEAHARGLRVLLDWV
                     PNHSSVRHPWFIEARSSRDNPRRDWYYWRDAGSDGSPPNNWQSEFGGPAWTWDEATGQ
                     YYYHAYLPQQPDLNWRNPDVQAAMLDTLRFWLDRGVDGFRIDALRQLMKDPDWRDNPP
                     NPDATPEMGLDNQLLPLHSADHADNDQIVRMIRAVLDEYPDRLLIGELYLPYERLMRY
                     YGTGGAGLHLPTNMHLIRTAWDAAHLAALIDRYERALPDHGWPNWVLGNHDRPRIASR
                     IGAAQARVAAMLLLTLRGTPTLYYGDEIGMRDVPIPPQRVRDPWGIRTGNPRLGRDPE
                     RTPMQWDASPNAGFCPPGVEPWLPIAGDAARNNVAVQRADSGSMLRLYQQLIALRRRE
                     PALQIGRYRPRGVDGNVLAYEREHDGRCLLVLLNLGARPHAWSSTDAPPAGHVLLSTN
                     PNRPDNHVAGTVALAADEGAVIDLT"
BASE COUNT          435 a          891 c          851 g          418 t
ORIGIN      
        1 tgacgcttcc agacgaagta gccggtcagc agcggcagga cgaacaggct ggcgttgcgg
       61 gcatagaagc tggcgtcctc gtcgatctgc atcccgaaca gggccgggat cttgatgagg
      121 aaggccgcaa cgatcgccag gcaaaaggcg acgacggcgt cggtccggac ccatgtccgg
      181 gtctcgccgg actcggccgg agcgacgacg agctgcttcc agaggcgctc cgagtgctcg
      241 cgggcgaact cgcgcgagag ggcgtcgagg ttgcccatcc gcttgacggc caccaggaac
      301 gcttcgtcgc tgtcgagtcc cgcctcgccg aggacggcga cctgctcgcg caggtggtct
      361 tccagctccg caacatcgac ggcgtggatc gcctggcggc ggcggaggta gttgcgccac
      421 tggccaatct ggtcttcgag cgggatggcg tcaccggcgg ctgccatcgt caggccccct
      481 gtggctgcgt cgccggctgg cccgccggcg ccggaaagga gagcacctgc cagatacccc
      541 gcagcgtcgc atcaaccgcc tgccactggc ggcgatcctc ggcgagctgc gaccggccct
      601 gaccggtgat ccggtaatac ttgcgccgcc ggccactctc ggccgtttcc cagcgtgccg
      661 caacatggcc gagccgctcc agccggtgga ggaccgggta gagcatcccg tccgtccact
      721 ccatctggcc gccggacagc tcgcgcacgc gctggaggat cgcgtagcca tagctgtcgg
      781 cttcggccag aatcgccagg acaatcgggg ttgaggacgc ggcgatcaga tccttgttga
      841 tctccatact gtcacccatc atcgatcgcg aagaagctcg cgctggagta catagcagca
      901 ttatacatcg cagtgtcggg tatacgaagg atcgcaagtt cgttgaagat ggatgcagga
      961 ccattctcgg gaacgggggg acagcagatg gcgagcgagc atcgctggtg gcagaccggt
     1021 atcgtctacc agatctatcc gcgctcgttc caggacagca acggcgacgg gatcggcgat
     1081 ctggcaggga tcaccagccg gcttgactac gtgcggtcgc tgggcgttga tgcgatctgg
     1141 atctcgccga tctacccgtc gccgatggcc gacttcggtt acgacgtcag cgaatacatc
     1201 ggcattgagc cggtcttcgg gacgctggac gacttcgacc ggctggtcgc cgaggcgcat
     1261 gcccgaggct tgcgggtgct gctggactgg gtgccgaacc attcgtcggt tcgacatccg
     1321 tggttcatcg aagcgcgctc ctcgcgcgac aaccccaggc gcgactggta ctactggcgg
     1381 gacgccggat cagacggcag cccgcccaac aactggcaga gcgagttcgg cggcccagcc
     1441 tggacctggg acgaggcgac tggtcagtat tactaccacg cctacctgcc gcaacagccg
     1501 gatctcaact ggcgcaatcc cgacgtgcag gcagccatgc tcgacacctt gcgtttctgg
     1561 ctcgatcgtg gcgtcgacgg tttccggatc gacgcgctcc gccagttgat gaaggacccg
     1621 gactggcgcg acaacccgcc caacccggac gccacgcccg agatgggcct cgacaaccag
     1681 ctcctgccgc tccattcggc ggaccatgct gacaacgacc agatcgttcg catgatccgg
     1741 gccgtgctgg acgagtatcc cgaccggctg ctgatcggcg aactgtacct gccctacgag
     1801 cggctgatgc gctactacgg cacgggaggc gcggggctgc atctgccgac taacatgcac
     1861 ctgatccgga cagcgtggga cgccgctcac ctcgccgcgc tgatcgatcg ctacgagcgc
     1921 gcattgcccg accatggctg gcccaactgg gtgcttggca accacgaccg cccccggatc
     1981 gcttcacgga ttggcgcggc ccaggcccgt gtcgcggcga tgctgttgct gaccctgcgc
     2041 gggacgccga cgctgtacta cggcgacgag atcggcatgc gcgacgtgcc gatcccgccg
     2101 cagcgggtgc gggacccgtg gggcatccgg accggtaatc cacggcttgg ccgcgatccc
     2161 gagcggacgc cgatgcaatg ggacgcgtcg ccgaacgccg gtttctgccc gccgggtgtc
     2221 gagccctggc tgccgatcgc cggcgacgcc gcgcgcaaca acgtcgctgt tcagcgggct
     2281 gacagcggct cgatgctccg gctctaccag cagttaatcg ccctgcgccg ccgcgagccg
     2341 gcgctccaga tcggccgcta ccgcccgcgc ggcgtcgacg gcaacgtcct cgcttacgag
     2401 cgcgagcatg atggccgctg cctgctggtc ctcctcaacc tcggtgcccg cccgcacgcc
     2461 tggtcgtcaa ccgacgcccc gccagccggt cacgtgctgc tctcgaccaa tcccaaccgt
     2521 cccgacaacc atgtcgccgg gacggtcgca ctggcagcgg acgagggcgc ggtgatcgac
     2581 ctcacctgat cgcgt
//
LOCUS       DASUGJ010000707         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1504966, whole genome shotgun sequence.
ACCESSION   DASUGJ010000707 DASUGJ010000000
VERSION     DASUGJ010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504966"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFV93_16030"
     CDS             complement(<1..1175)
                     /locus_tag="VFV93_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166915.1"
                     /translation="MKNRSHWLLGAAVAVALIGSACGDNDNNNSAGNATPASSAPAAA
                     VATMTPQSSMAPTSNTKAAELRTVLNGLLSEHVALASSATAGALGGRQAQFDGAAAAL
                     DANSVDLSKAIGSVYGAQAEQAFLPLWRKHIGFFVDYTQGVATKDQAKQQKAVADLTQ
                     YTQDFGAFLAAANPNLPKDTVAELVKEHVLTLKAVVDAQASGDQAQQFSAIREAYGHM
                     HMIADPLAGAIATQFPDKFPGDAGSKASTLRSQLNTIEREHVYLAARATGAALGGREA
                     EFKAAADALDANSVDLSKMIGSAYGTDAERAFLPSWRKHIGFVVDYTQGLAAKDQAKQ
                     QKAVADLTSYSQDLGAFLNSANGLPKDAVAALVMDHILTLKTVIDAQAAGDQPKVYD"
     gene            complement(1293..1679)
                     /locus_tag="VFV93_16035"
     CDS             complement(1293..1679)
                     /locus_tag="VFV93_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX5166916.1"
                     /translation="MTVRGARWQWFVGLIALLGLLALSGCSGSDSPPASAATADTGNS
                     PVAIRLFQFQPSPLEVTAGTTVTWTNGDDILHTVTSGAPGAKDGRFDGTLDGSGTSYM
                     VTFSEPGTYAYFCSRHESMRGEVRVN"
     gene            1780..>2331
                     /locus_tag="VFV93_16040"
     CDS             1780..>2331
                     /locus_tag="VFV93_16040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HEX5166917.1"
                     /translation="MTTDAQAPEGIALVSSDGLCFYARMRIGLITDTHLPALIRSLDD
                     LGPEAGAFFRTVDLILHAGDVTAPSVLDWLDHFAPVVVAQGNNDDFRDPRMKPIQMLD
                     VHGWRIGMVHNLAPETRPIDAMRSRHFNDESVDIMIGGHTHLERLEVRDGVLIVNSGS
                     PTLPHHKETRLGTLGLLEFDGDHL"
BASE COUNT          403 a          740 c          738 g          450 t
ORIGIN      
        1 ctatcgtaga ccttcggctg atcgccggcc gcctgggcgt cgatcacggt cttcagcgtg
       61 aggatgtggt ccatcaccag cgcggcgacg gcatcctttg gcaggccatt cgccgaattg
      121 aggaacgcgc ccaagtcctg gctgtatgac gtgagatcgg cgaccgcctt ctgctgcttc
      181 gcctggtcct tcgcggccaa cccctgagta tagtcgacga cgaagccgat gtgcttccgc
      241 cacgacggca agaaagctcg ctcggcgtcg gtgccgtagg ccgaaccgat catcttcgac
      301 aggtcaaccg agttggcatc gagcgcgtcg gccgctgcct tgaactcagc ttctcgcccg
      361 ccgagcgcgg cgccggtggc acgggcggcg agatagacat gctctcgctc gatcgtgttc
      421 agttgcgaac gcagggttga ggccttcgat cccgcgtcgc cggggaactt gtcggggaac
      481 tgcgttgcaa tcgcccccgc cagcgggtca gcgatcatgt gcatgtgccc gtaggcctca
      541 cggatcgcgg agaactgctg cgcctggtcg ccggaggcct gggcatcgac gaccgccttc
      601 agcgtaagga catgctcttt caccagttcg gcaacggtat ccttcggcag gttcggattg
      661 gcggcggcaa ggaacgcgcc gaagtcctgg gtgtactgcg tcaggtcagc cacggccttc
      721 tgctgctttg cctggtcttt cgtggctaca ccttgggtgt agtcgacaaa gaagccgatg
      781 tgcttccgcc agagcggcag gaacgcctgc tccgcctgcg cgccgtagac cgatccgatc
      841 gccttggaga gatcgaccga gttggcgtcg agcgcggcgg ccgcgccatc gaactgcgcc
      901 tgacggccac ccagagcgcc ggctgtggcg ctggaggcca gtgccacgtg ctcgctgagc
      961 aggccgttga gtaccgtccg cagctccgcc gccttcgtat tggacgtggg cgccatcgac
     1021 gactgtggcg tcatcgtcgc caccgccgcg gccggtgcac tggatgccgg cgtagcattg
     1081 ccggcgctat tgttgttgtc gttgtcgccg caggccgagc cgatcagcgc gactgcgacc
     1141 gcggccccga ggagccagtg tgatcgattc ttcattgtgt tcccttctca tgtgtcctgg
     1201 gcggacctgc atcgtgctct gaggtgcggc agacgccgag tgctcgtcgt ggaccgaacg
     1261 ccctcctttc ccctcgggag agggtggttt cgttagttga cgcggacttc accgcgcatc
     1321 gattcgtgcc gggaacagaa gtatgcgtag gtgcccggct cagagaacgt gaccatgtag
     1381 ctcgtaccag aaccgtccaa cgtgccatcg aagcgtccgt ccttggcgcc aggtgcgccg
     1441 gacgtaacgg tgtgcaggat gtcgtcaccg ttggtccacg tgacggtcgt tccagcggtc
     1501 acctcgagtg gcgaaggctg aaactgaaac aggcgaatcg ctaccggcga attgccggta
     1561 tctgccgttg ctgctgaggc gggaggggaa tccgaaccac tgcaaccaga caaagcgagc
     1621 agaccgagca gagcgatcaa accaacgaac cattgccatc gagcgccgcg tactgtcatg
     1681 ccaaacctcc gttgtccgct actacacctg gtgctacgga cgctggtcgg ccggcggatc
     1741 acggcggttg acggccggat cagggattcc acgtgagatg tgaccacgga tgcacaggcc
     1801 ccggagggca tcgcattggt gtcttcggat ggattgtgct tctatgcacg catgcgcatc
     1861 ggcctgatca ccgacactca cctacctgcc ttgattcgct cgctcgatga ccttggcccc
     1921 gaggccgggg ccttctttcg cacggtggac ctgatcctcc acgccggcga tgtgaccgca
     1981 cccagtgtgc tcgactggtt ggaccacttc gcgcccgtcg tcgttgcgca ggggaacaac
     2041 gacgactttc gcgacccgcg gatgaagccc atccaaatgc tcgatgtgca tggctggcgg
     2101 atcggaatgg tgcataacct tgcgcccgaa acacggccga tcgacgcgat gcgaagccgg
     2161 cacttcaatg acgagtcggt cgatatcatg atcggcggtc acacacacct ggaacggctc
     2221 gaagtgcgcg acggcgtcct catcgttaac tccggtagcc caaccttgcc ccaccacaag
     2281 gagacacggc tcggcaccct cggcctcttg gagtttgatg gcgaccatct a
//
LOCUS       DASUGJ010000708         4620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1646872, whole genome shotgun sequence.
ACCESSION   DASUGJ010000708 DASUGJ010000000
VERSION     DASUGJ010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646872"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFV93_16045"
     CDS             complement(<1..486)
                     /locus_tag="VFV93_16045"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5166918.1"
                     /translation="MRRLARTCYQHRRLVLTGWIVLLIGLFALSSVAGGESKTEFSLP
                     GSESQEAFDLLEQHGFANRAGEQAQIVFEAEQGVNDPAVKQAMEALFATVGEEVADAQ
                     VNSPYAPGGERQISPDGTIAYAEVNFTDRSQEEYREAGDQIREHAEEIDVSGLRIEFG
                     GY"
     gene            603..1064
                     /locus_tag="VFV93_16050"
     CDS             603..1064
                     /locus_tag="VFV93_16050"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5166919.1"
                     /translation="MQAALRGVHNAVDTLSQIIADQLGVNRTDLRCIQMLGQFGPLTA
                     GQLAEASGLTTGALTSVIDRLERAGFARRVRDEVDRRRVYVELTPSAGQRMGAIYRDL
                     VIASAASMAGYSDAELILIRDFMLQGREVTLAHVERVKAMSSKLDAAAGSG"
     gene            1155..2420
                     /gene="purD"
                     /locus_tag="VFV93_16055"
     CDS             1155..2420
                     /gene="purD"
                     /locus_tag="VFV93_16055"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX5166920.1"
                     /translation="MATNVLVVGGGAREHALVWKLAASNRVGQLYCAPGNAGTLSLAE
                     NLPLTDFDTLANEIEARGIDLTVVGPEAPLADGLADVLSARGHVVFGPSAAAAKIESS
                     KAWAKEIMASAGVPTAQAPTCDTLRAALDMVYDTPLPLVVKADGLAAGKGVIICETRA
                     EAEAAVREMLEARMLGDAASTVLIEEFLTGQEVSFLALTDGESVLPLLPACDYKKIGD
                     GETGPNTGGMGAYTPPGSVDAAMQQRFLDEVITPVVRELANRGIQYQGVLYAGMILTP
                     DGPKVLEFNCRFGDPETQVILPMLNADFVDLCEATARGELAALPPIDWFPGACAGVVL
                     ASGGYPGPYTSGHPIDGLDAIPDGGIVFHAGTIRRGSETVTAGGRVLTCVGRGPDMAA
                     ARALAYATADAITFDGVYRRGDIALREMM"
     gene            complement(2567..3283)
                     /locus_tag="VFV93_16060"
     CDS             complement(2567..3283)
                     /locus_tag="VFV93_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166921.1"
                     /translation="MSNQRAYKTGTVMIIDDHPLFRQGIRRLIEESGRYTVVAEATNG
                     YEAIREADIHEPQIIVSDIQLPGVTGLKISRILKKQHPKSQIIFLSMHMDDERLFDAV
                     RAGAAAFLTKDVNAEDLIGTIDAVCRGEQPINTTLLTRPQLAWKVLSEFRTMTDDPAA
                     RYKSAMPLSVREVEVLDCVSQGLSNKEVADALYITEQTVKNHMTSVLRKLNVEDRVQA
                     VLFAVKNGWVEIGPGIYSAA"
     gene            complement(3578..3850)
                     /locus_tag="VFV93_16065"
     CDS             complement(3578..3850)
                     /locus_tag="VFV93_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166922.1"
                     /translation="MSDRCYLQPQLVAFACGLALVVPQHVAFVDGSQQFACFAAEQHD
                     ESGAACVPLTAVAPAGTAWMSVVPYSLAIEVFLSRSTHDLYRLRYK"
     gene            complement(3966..4400)
                     /locus_tag="VFV93_16070"
     CDS             complement(3966..4400)
                     /locus_tag="VFV93_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166923.1"
                     /translation="MQLPSPPLDATLQPRYRNLLHRRRLSLSQPFELGQRQCVSIRRG
                     ELVEQRCEAGNKFLDGSRVISVKVRCLDGVELCIGPEPFPSSSRRSVVVEDAVARYLV
                     DPSMDTVLVLDSVDSFMDTNEDILHDVVDIGRVLDPSSNECP"
BASE COUNT          798 a         1487 c         1515 g          820 t
ORIGIN      
        1 gtagccgccg aactcgatcc gcaggccgga gacatcgatc tcctcggcgt gctcgcgaat
       61 ctggtcgccg gcctcgcgat actcctcctg gctgcgatcg gtgaagttca cttcggcgta
      121 ggcgatcgtg ccgtccggcg agatctgccg ctcgccgccg ggcgcgtagg ggctgttgac
      181 ctgtgcgtcg gcgacctctt cgccgacggt cgcgaagagc gcctccatcg cctgtttgac
      241 ggccggatcg ttgacgccct gctcggcctc gaagacgatc tgggcctgct cgccggcccg
      301 gttggcgaag ccgtgctgct ccagcaggtc gaaggcctcc tgactctcgg agccgggcag
      361 cgagaactcg gtcttcgact cgccgccggc caccgaactg agggcgaaca gcccgatcag
      421 caggacgatc cagcccgtca ggacgagccg tcggtgctgg tagcaggtac gtgcgagacg
      481 tctcatcgtt gaggttccct ccattggacg tgtatactgc tgtacagact agttatctta
      541 aagatcgaga gattagattg tcaaggcacc cggaggattc acgccagcaa ctcattggcg
      601 acgtgcaagc cgcgctccgc ggcgttcaca acgcggtcga taccctgagc cagatcattg
      661 ccgaccagct cggcgtcaac cggaccgacc tgcgctgtat ccagatgctc ggccagttcg
      721 gcccgctgac ggccgggcag ttggctgagg cgtccgggct gacgaccggg gcactgacga
      781 gcgtcatcga tcggctggag cgagccggct tcgcgcggcg ggtccgcgat gaggttgacc
      841 gccgccgcgt ctacgtcgaa ctgacaccgt ccgccggcca gcggatgggc gccatctacc
      901 gcgatttggt catcgcctcg gccgcctcga tggcgggcta cagcgacgcc gaactgatcc
      961 tcattcgtga cttcatgctc cagggccggg aggtgacgct cgcgcacgtc gagcgcgtca
     1021 aggcgatgtc gagcaagctc gatgccgccg ccggctccgg ctgacctacc cttcagcacg
     1081 cgccatacga tagattcatg ccagcaggcg ccggttgggt tgctcctgcg tcggtagcgt
     1141 aggagggcgc gggaatggca accaatgtcc tggtcgtcgg gggtggggcg cgggagcacg
     1201 cgctggtctg gaagctggcg gccagcaacc gcgtcggcca gctctactgc gcgccgggca
     1261 acgccgggac gctctcgctg gccgagaatc tgccgctgac cgacttcgac accctcgcca
     1321 acgagatcga agcgcgcggg atcgacctga ccgtcgtcgg gccggaggcg ccgctggccg
     1381 acggcctggc cgacgtgctc tcggcgcgtg gccacgtcgt cttcggcccg agcgccgccg
     1441 ccgcgaagat cgagagcagc aaggcctggg cgaaggagat catggcctcc gccggcgtcc
     1501 cgaccgcgca ggcgccgacc tgcgacaccc tccgggcagc gctcgacatg gtctacgaca
     1561 caccgctgcc gctggtcgtc aaggccgacg gtctggcagc cggcaagggt gtcatcatct
     1621 gcgagacgcg cgccgaggcc gaggcagccg ttcgcgagat gctggaggca cgaatgctcg
     1681 gcgacgcggc ctcgaccgtc ctgatcgagg agttcctgac cggtcaggag gtctcattcc
     1741 tggcgctgac cgacggcgag tcggtcctgc cgctgctgcc ggcctgcgac tacaagaaga
     1801 tcggcgacgg cgagaccggc ccgaacacgg gtgggatggg cgcctacacg ccgccggggt
     1861 ctgtcgacgc cgccatgcag cagcgtttcc tcgacgaggt catcacgccg gtcgtccgcg
     1921 agctggcaaa ccgcggcatt cagtaccagg gcgtcctcta cgccgggatg atcctgacgc
     1981 cggacggccc gaaggtgctc gaattcaact gccgcttcgg cgacccggag acgcaggtga
     2041 tcctgccgat gctcaacgct gacttcgttg acctctgcga ggcgaccgcc cgtggcgagc
     2101 ttgccgcgct accgccgatc gactggttcc ccggcgcctg cgccggcgtc gtcctcgcct
     2161 ccggcggcta ccccggcccc tacacctcag gccacccgat cgacggcctc gacgccatcc
     2221 ccgacggtgg catcgtcttc catgccggca cgatccgccg tggcagcgag accgtcacgg
     2281 caggtggccg cgtcctcacc tgcgtcggcc gcggcccaga catggccgcc gcccgcgccc
     2341 tcgcctacgc aaccgccgac gcgatcacct tcgacggcgt ctaccgccgc ggagatatcg
     2401 cgttgcggga gatgatgtag ttcgggattc gggattcggg ggcggtgaac gggaactgtc
     2461 ccgcgacacc cccgaatccc gcgtcccgaa tcccgaacac agcgacgccg gcccgaacat
     2521 tcgggccggc gtcgcatggg tgggaggagg ggaggattat tcggaattac gcggcagaat
     2581 agatgccggg accaatctcg acccaaccgt tcttgacggc gaagaggacg gcctggacgc
     2641 gatcctcgac attgagcttg cgcaggacac tggtcatgtg gttcttgacg gtctgctcgg
     2701 tgatgtagag cgcgtcggcg acttccttgt tcgagagtcc ctgtgagacg cagtccagca
     2761 cctcgacctc gcggaccgag agcggcatgg ccgacttgta gcgcgcggcc gggtcgtcgg
     2821 tcatcgtccg gaactcgctg agcaccttcc aggcgagctg cgggcgggtc agcagcgtcg
     2881 tattgatcgg ctgctcacca cggcagacgg cgtcgatcgt gccgatcagg tcctcggcgt
     2941 tgacatcctt ggtcaggaag gcagccgccc cggcccggac ggcgtcgaac agccgctcgt
     3001 cgtccatgtg catcgagagg aagatgatct ggctcttcgg gtgctgcttc ttgagaatgc
     3061 gtgagatctt gaggccggtc acgcctggaa gctggatgtc ggagacgatg atctgcggct
     3121 cgtggatgtc ggcctcgcgg atcgcctcgt agccattggt ggcctcagca acgaccgtgt
     3181 agcgcccgct ctcttcgatc aggcggcgaa tgccctgcct gaacagcgga tggtcatcga
     3241 tgatcatgac ggttccggtc ttgtacgcgc gctggttgct catgaccgct ctccttcgtg
     3301 tcgagtgacg ccgtccctgg cccggcgttc cgtcactcgc ccgtatcgcc cccggtggga
     3361 tgtcccacat cccgctccca aatatccgac ttgcgtactt cagatgatag ggtcgggcta
     3421 tcaattgggg tatggggcaa agcgcacgat cgatcggtga gggagtacta gtaccgaaaa
     3481 gtactcaggt catgggtgct tcaagcactc gacatatgga ggcttatcac gctctgattg
     3541 cgtccttttg ccggtcacac cgtctctaca gatagcgcta cttgtagcgc aatctgtaga
     3601 gatcgtgagt cgatcgagaa aggaacacct caatggcgag tgagtatggt acgacggaca
     3661 tccaggccgt cccggcgggc gcgacggcag tgagcggtac gcaagcagcg ccgctctcgt
     3721 cgtgttgctc cgcagcgaag caggcgaact gctgcgagcc gtcgacgaag gcgacgtgct
     3781 gcggaacgac gagtgccagc ccgcaggcaa aagccacgag ctgcggctgc aagtagcatc
     3841 ggtctgacac cgcgccgtat cctttccagg cgcggtgtca cccgttctca tgcgcgagtg
     3901 agggaggatc ctccagcgag atggtagcga cgactgaggt cctctgggac gcgtttcacc
     3961 aacgactacg gacattcgtt gctcgacgga tcgagaaccc tgccgatgtc gacgacatcg
     4021 tgcaggatat cttcgttcgt atccatgaac gaatcgacgc tgtcgagcac gaggaccgtg
     4081 tccatgcttg gatctaccag atagcgcgca acagcatctt cgactactac agagcgccga
     4141 gacgacgacg ggaacggctc gggtccgatg cagagttcga cgccgtccag gcagcggacc
     4201 ttgacgctga tgacccggct gccgtcgagg aacttgttcc ctgcctcaca ccgctgttcg
     4261 accaactcac cccttcgtat cgagacgcac tggcgttgac cgagttcaaa gggttgactc
     4321 agcgagaggc ggcgtcggtg cagcagattt cggtatcggg gctgaagagt cgcgtccagc
     4381 gggggcgacg gcagctgcac gatctgctgc gtgcgtgctg tgagattgag cttgaccggc
     4441 gtggcggagt gatcaactac agaacacggg aatcaacctg cgcttgttgc cccgcatgtg
     4501 atgacagcag ccctcagtag gttgtgcccg gctccaagat cacaatttgc tggcattcgg
     4561 tgccaatgct agccgtcgac cggatacgta gcgaggtatc cgcaacgtga ggcaatcggg
//
LOCUS       DASUGJ010000709         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1089367, whole genome shotgun sequence.
ACCESSION   DASUGJ010000709 DASUGJ010000000
VERSION     DASUGJ010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089367"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VFV93_16075"
     CDS             <1..984
                     /locus_tag="VFV93_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5166924.1"
                     /translation="LLGVPHNAPDAAVAARDKFKMRSMLAAGGVRCPGFRTFPAGADP
                     RTYANEVTYPSVVKPTRLSGSRGVIRANNADELIAAVNRLRAILTSDGTDLTAAEILI
                     EDYMPGEEVAVEGLLTDGELHVLTIFDKPDPLVGPFFEETIYTTPSRLPVEVQEAIAR
                     ETAACAAALGLRTGPVHAELRVNEQGPWILEIAGRSIGGLCSTVLSFGAGMCLEELIL
                     RHAVGMEIPSYDRTGDAAGVMMIPIPKSGILRSVDGVAEASAVPHIDGIEITAKLNYP
                     IKPLPDGASYLGFIFARADTPQEVEQALRDAHAKLRIRIDPMMVMQMTAAG"
     gene            complement(1002..1307)
                     /locus_tag="VFV93_16080"
     CDS             complement(1002..1307)
                     /locus_tag="VFV93_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX5166925.1"
                     /translation="MVNCVKLGRELPGLEAPPFPGELGLRIYENVSAEAYQLWGQHAT
                     IMINHYGLHPADPDTRKLLRKEMEEFFFGENARMPDDWVAPGAAPSGKGGGAPARKK"
     gene            1579..2295
                     /locus_tag="VFV93_16085"
     CDS             1579..2295
                     /locus_tag="VFV93_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX5166926.1"
                     /translation="MASTAPASVFHDLPTYQVGRLTSSGTLELIDQLDPDLIVVACFP
                     RLIPEAIHGRARLGGINIHPSLLPQHRGPDPLFWIMRDGGRGCGVTVHALADRYDSGD
                     ILAQRPVPYPAGVRESELERRLAGEGAELALSVIEGLVVGRQSRTAQDEHQASYESWP
                     AERDYHLDTRRSALDSWNFVRGVAERGVPIRIDAGDLLIREAIAFGDDTEPPEAGDGE
                     LIIRCNPGWLRAVIDDETDR"
     gene            2454..>2992
                     /locus_tag="VFV93_16090"
     CDS             2454..>2992
                     /locus_tag="VFV93_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166927.1"
                     /translation="MTISLDRAYAEALDRDDPLASFRDRFYVQPGTIYLDGNSLGLLS
                     KDAEQRLQSVLNDWKQLGIDGWLRADPAWFYLGEQLGARLAPMFGADVDEVIVTGTIT
                     VNLHQLIATFYNPEGTRRKIIATSLDFPSDIYAMQAHIGRHGGDPDGDLIKVPSPDGR
                     IIEEDDIIAAMTDEVALILL"
BASE COUNT          534 a         1022 c          918 g          518 t
ORIGIN      
        1 ctgctcggcg tcccgcacaa cgcgccggac gccgccgtcg ccgcccgcga caaattcaag
       61 atgcgctcga tgctggctgc cggtggcgtc cgctgtcccg gcttccgaac cttcccggcc
      121 ggcgccgatc cgcgcaccta cgcgaatgag gtcacctacc catcggtcgt gaagccaacg
      181 cggttgtctg gcagtcgcgg cgtcatccgc gccaacaacg cggacgaact catcgcggcc
      241 gtcaatcgac tccgagccat cctgaccagc gacggcaccg acctgaccgc ggccgagatc
      301 ctcatcgagg actacatgcc gggagaagag gtcgccgtcg aagggctgct gaccgatggc
      361 gagctgcacg tcctgacgat cttcgacaag ccggatccgc tggtcggccc gttctttgaa
      421 gagacgatct acacgacacc ctcacggctg ccggtcgagg tccaggaggc gatcgcccgc
      481 gagacggccg cctgcgcagc cgcgctcggc ctgcggaccg ggccggttca cgccgagtta
      541 cgggtcaacg agcaaggacc gtggatcctt gagattgccg gtcggtcgat cggcggcctc
      601 tgctcgaccg tcctctcatt cggcgccggg atgtgtctgg aggagttgat cctgcggcac
      661 gcggtcggga tggagatccc gtcctacgac cggaccggcg acgcagccgg cgtgatgatg
      721 atcccgattc cgaagagtgg catcctgcgc tcggtcgacg gcgtggctga ggcaagcgcg
      781 gtcccgcaca tcgacgggat cgagatcacc gccaagctga attacccgat caagccgctg
      841 ccggatggcg ccagctatct tggcttcatc ttcgcccgcg ccgacacgcc gcaggaggtg
      901 gaacaggccc tgcgcgacgc acacgccaaa cttcggatcc gaatcgaccc gatgatggtg
      961 atgcagatga cggcggctgg gtgaccgtcg gtcgtgagac gctacttctt gcgggctggt
     1021 gcgccgccac ccttgccgga gggagccgcg ccgggggcga cccagtcgtc cggcatccgc
     1081 gcgttctcgc cgaagaagaa ctcttccatc tccttgcgca gcagcttgcg ggtgtccgga
     1141 tcagccggat ggagaccata gtggttgatc atgatcgtcg cgtgctggcc ccagagctgg
     1201 taggcctcgg ccgagacgtt ctcataaatg cgcaggccca gctcaccggg gaacggaggc
     1261 gcttcaagac ccggcaactc gcgtcccagc ttgacacaat tcaccatccg agccacgccg
     1321 gcctccctca tcgactagct cgcgatcccg caccggcgct tcgccgatcg tcgggtcgcg
     1381 agtcctacga ctatgaaaac tgtatcacct gacgtgccac cagcgcgcgt tctgcttttc
     1441 gggatccgct gccagttcac tgagacggtc gccgcacggc tttccggcag taagtaccag
     1501 ccagtcggcc tgattctccc cggtccgccg ggaatcgagc aacccattcg cctctcgcca
     1561 cgtcaaaacg tgctcccgat ggcgtccacc gcaccggcca gcgtttttca cgacctcccg
     1621 acctaccagg ttggacgatt gacgtcttca ggaacactcg aactcatcga tcagttagac
     1681 cccgatctga tcgtcgtcgc ctgcttcccg aggctgatcc ctgaggcgat tcacggccgc
     1741 gcccggctcg gtggtatcaa catccaccca tcgctgctcc cccaacatcg cggccccgac
     1801 ccactcttct ggatcatgcg cgacggtggc cgtggctgcg gtgtcactgt ccacgccttg
     1861 gcggatcgat acgacagcgg cgatatcctc gcgcaacggc ccgtgccgta tccagccggg
     1921 gtccgcgaga gtgaactcga gcgacggctc gctggtgagg gagccgaact ggcactcagc
     1981 gtcatcgagg gactcgtcgt gggccgtcag tcgcgaactg cgcaggacga gcaccaggcg
     2041 agctacgaaa gctggcccgc tgaaagggac tatcacctgg atacgcgccg ttcggccctc
     2101 gacagctgga acttcgtacg gggcgttgcc gagcggggcg taccgatccg gatcgatgcc
     2161 ggcgacctgc ttatccgcga agccatcgcc ttcggcgacg atactgaacc tcctgaggca
     2221 ggcgacgggg aactcatcat ccgatgtaat cccggctggc tacgggctgt catcgatgac
     2281 gagaccgatc gttgacgagg accagaccga gtgacgacga gctcacgtgc tggcacggcc
     2341 aacgatcatc gtgatgcgcg accctccgac cttccagcac gacttcggag agcatcacgc
     2401 tcgatgatgg tattctcccc tcatcgtgtc gcacgcatcg ggagggacgc agcatgacca
     2461 tcagcctcga tcgcgcctac gcggaggcac tcgaccgtga cgatccgctc gcgtcgttcc
     2521 gcgatcgctt ctacgtccag ccggggacga tctacctgga cggcaactcg ctcggcctgc
     2581 tctcgaagga cgccgagcag cgccttcagt ccgtcctcaa tgactggaag cagttgggca
     2641 tcgacggctg gctgcgcgcg gatccggcct ggttctatct gggtgagcag ctcggagcgc
     2701 gactggcgcc gatgttcggg gcggatgtcg acgaagtcat tgtcaccggc acgatcaccg
     2761 tcaacctgca tcagctcatc gcgaccttct acaacccgga aggtacccgt cgcaagatca
     2821 tcgcgacctc gctcgacttc ccctccgata tctacgcgat gcaggcccac atcgggcggc
     2881 acggaggcga ccctgacggc gatctgatca aggtgcccag cccggacggt cggatcatcg
     2941 aggaggacga catcatcgcc gcgatgaccg acgaggtcgc gctgattctg ct
//
LOCUS       DASUGJ010000710         7171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_811747, whole genome shotgun sequence.
ACCESSION   DASUGJ010000710 DASUGJ010000000
VERSION     DASUGJ010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811747"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VFV93_16095"
     CDS             <1..699
                     /locus_tag="VFV93_16095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5166928.1"
                     /translation="HRLAREIHDGPAQALANAIIALEFVERAIRAGGEGSAERAQEEV
                     ERIKSTLREGLTEIRRFIFDLRPTMLQDRGLVATVEHYIATYQSLFPMAIEFHAPDAL
                     ARLTVDQELTAFRVIQEAIQNARKHARASRVNVDVRLREAVLAVTIEDNGRGFSPDRV
                     TTHIMGGAGLRGMRERAALVGGDIEIESAPGEGTKIVLSLPISGTEAPPQAFDDQVRP
                     VDNSDQANAGMKAD"
     gene            802..1494
                     /locus_tag="VFV93_16100"
     CDS             802..1494
                     /locus_tag="VFV93_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5166929.1"
                     /translation="MIVDDHPLFRRGIRWSLESESDLVVGAEAADGHEALAMADRISP
                     DVVLLDINLPGMSGIEVARLLKRRHPRLGIVILTMHEDDEQLFNAIRVGAAAYCTKDV
                     DPSLIVGIIRRVARGEYLINENVLSHPFVASRVLDQFRELARMDQESDSVFSPLTPRE
                     VEILDCVARGNSNKEIAVFLGISDQTVKNHITSILRKLQVNDRTQAVIYALRHGWIKL
                     EGDEAPPGTPKA"
     gene            complement(1603..3342)
                     /gene="mutL"
                     /locus_tag="VFV93_16105"
     CDS             complement(1603..3342)
                     /gene="mutL"
                     /locus_tag="VFV93_16105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX5166930.1"
                     /translation="MSIQRLTLETVGKIAAGEVIERPAAAVKELLENAIDAGGRRIDI
                     AIAGGGIDELEVRDDGRGIAFDDLPLAIERHATSKIASIDDLKALTTLGFRGEALASL
                     SAISDLRIQSLDEGETAGGMLRVEFGHDRPPQRVAWGGGTSIVARDLFANLPARRKFL
                     RQPQTEANYVARIVSAYALAYPQIAFSLEIDGRRSFVTDGSGDTIGAAVAVWGPEIAG
                     SLVPLVNPDETPDGFEVSGIVSLPDLDRATRQQQYLFAQGRLITSRQLGTAFEQAYHT
                     LLMVGRHPIGCIQIRVPADRIDVNVHPTKAEVRFADERLAFALVQRATREAIVGHAAP
                     AVIPTLVSSPLDEWTVQRRFSLAHPERHLEPTGDRPTQPVSEQDFEFGAAPVGGRRLP
                     VLRVLGQVASMFIIAEGPDGMYLIDQHAAHERILFEKLMPGFELRASDRQLMLQPVQM
                     QLAARDWETFDACRDELAAVGFEIEEFGGGSVLIRSVPAVLKIREPAKVLAKMLDEMG
                     GGGRGTSRLESLAISAACHTSIRAGQALSLLEMRELVSQLEACSSPLACGHGRPTMLR
                     MTAEELEKQFSRR"
     gene            complement(3339..3878)
                     /locus_tag="VFV93_16110"
     CDS             complement(3339..3878)
                     /locus_tag="VFV93_16110"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX5166931.1"
                     /translation="MVSSRYLRCDAFAGEVTGIAHDIIGRSLVVRRGAVEIAGLIVET
                     EAYGGVEDPASHAAFRPGGRAAAMVGPPGMVYVYSAYGVYPCFNIVTGPEGEPSAVLL
                     RAVQIEGEQRVTSGPGRLTRAMGIDSGDHGALVCGERFGITAARVALTIVATPRIGIT
                     RGVDLPWRFLGTAIEATRA"
     gene            complement(3871..6549)
                     /locus_tag="VFV93_16115"
     CDS             complement(3871..6549)
                     /locus_tag="VFV93_16115"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX5166932.1"
                     /translation="MAARQKSSNRARELGKAYEPAAAEQHWYQFWQSGGYFKPVDRGR
                     GPFVIVMPPPNVTGELHMGHALFSAVEDILTRYKRMQGHAALWLPGADHAGIAGQWVV
                     ERELAREGKSRHDLGREEFVARIWDWMETYQERIKGQLRLLGASADWSRYVFTMDPGP
                     ARAVRVAFKHLYDKGLIYRGERIISWCPRCMTALSDLEVVHEDEQSFLWHLRYPLADG
                     SGAVEVATTRPETMLGDTGVAVHPDDERYKDLVGKELILPIVGRRIPIVADAIVDPEF
                     GSGAVKVTPAHDPNDFEIGRRAGLPAINIMNLDGTMNAEAGPFAGQTIAEARKNVVKR
                     LEEDGALVRIEPHTHSVGHCDRCGSVVEPMISKQWFVSMEPLAKPAIEVVKDGTVRFI
                     PDRFSSVYLNWMENIHDWCISRQLWWGHRIPIWYCDTCGEVTATDQEVLTACGHCGSD
                     QIRQDPDVLDTWFSSGLWTFSTLGWPDQTPDLEYFYPGHVMETGYDIIFHWVARMIFF
                     GIEFMGEAPFRDVYLHGTVRDADGQRMSKTKGNVLDPTLATAEYGSDALRFALITASG
                     PGNDLKMSNERVESMRNFANKLFNATKFALRAIEGAEIERDADGAPLMPDADALAVAD
                     AWIIDRLHHTIAEVTRQVDEYQLHEAGRSLYEFIWSEFCDWYIEAAKVRLYADTPDPA
                     VPQTLAYVLERTLRLLHPFMPFVTEELWQHLPHPGEALIVAPWPEAGQRFASEAEAFA
                     AIAEAVRLIRNARAEQGVDTARRIPAIVYPGPATEAFDALRGELISLARLDADGLEYR
                     AGEPEAQEGSVAIVSGVSSIFLPLKGMIDVEAERARITREIEQVEGEISRATAMLANE
                     QFVSRAPANVVEGHRAKLAGAEERLALLQARLADLG"
     gene            complement(6975..>7171)
                     /locus_tag="VFV93_16120"
     CDS             complement(6975..>7171)
                     /locus_tag="VFV93_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166933.1"
                     /translation="QLVAWDLDINVLEVVLAGATDDDSIDGHLVSGLCKRGPSAGLRL
                     VSVRRTSIRMQIVAVKRRLR"
BASE COUNT         1273 a         2530 c         2125 g         1243 t
ORIGIN      
        1 caccggctcg cccgcgagat ccacgacggc cccgcccagg cgctggccaa cgcgatcatc
       61 gcgctggaat tcgtcgaacg ggccatccgg gccggcggcg aaggttccgc cgaacgggcc
      121 caggaggaag tcgaacggat caagtccacc cttcgcgagg ggctgaccga gatccggcgc
      181 ttcatcttcg atctgcggcc gacgatgctt caggatcgtg ggctggttgc gaccgtcgag
      241 cactacatcg caacctatca gtctctcttt ccgatggcga tcgagttcca cgcacccgat
      301 gccctcgcgc ggctgacggt tgaccaggaa ctgacggctt tccgggtcat ccaggaagcg
      361 atccagaacg cacgcaagca cgcccgtgcc agtcgtgtca acgtcgatgt ccgcctgcgc
      421 gaagcggtgc tggccgtgac aatcgaggac aacggccgcg gattttcgcc cgaccgcgtg
      481 acgacccaca tcatgggcgg cgccggtcta cgcggcatgc gcgagcgggc tgccctcgtc
      541 ggcggcgaca tcgagatcga gagcgcgccg ggcgaaggca ccaaaatcgt gctctctctc
      601 cccatttccg gtactgaagc acccccgcaa gcgtttgacg atcaggtcag accagttgac
      661 aatagtgatc aagccaacgc cggcatgaag gccgattgat agttcattcg cgacgttttt
      721 cgtcgtcgag acgaccggta gtgtagacgt ccgctcgaca gacaggtgta gcaaatgcat
      781 ccattgcaac gagtccagat attgatcgta gacgaccatc cactcttccg gcgcggtatc
      841 cgttggagcc tggagagcga atccgatctc gtcgttggcg ccgaggccgc agacggccac
      901 gaggctctgg cgatggccga tcgcatcagc ccggatgtcg tcttgctgga catcaacctg
      961 cctggcatga gcgggatcga ggtcgcccgt ctgctcaagc ggcgccatcc gcgcctgggg
     1021 atcgtgatcc tgacgatgca cgaggacgat gagcagctct tcaacgcgat ccgtgttggc
     1081 gccgccgcct actgcacgaa agacgttgac ccatccctga tcgtcggcat catccgccgg
     1141 gtcgcgcggg gcgaatatct catcaacgag aacgtcctct cccacccgtt cgtcgcctcg
     1201 cgcgttctcg atcagttccg tgagctggcc cgcatggacc aggaatccga ctcggtcttc
     1261 tccccgctta ccccgcgcga ggtggagatc ctcgactgtg tcgcgcgcgg aaacagcaac
     1321 aaggagatcg ccgtcttcct cggcatctcc gatcagaccg tcaagaatca catcacgtcg
     1381 atcctgcgca agctacaggt caacgaccgc acgcaagccg tcatctacgc cctgcgccac
     1441 ggctggatca agctcgaagg cgacgaagca ccgccgggga cgccgaaggc gtaacgatct
     1501 gtcgtcctga gccgcagcga aggaccgccc agcgttagcc ttgtgtggcg ctcaaacgga
     1561 gaggtccctc gctgcggctc aggacgacag atcgttacgc cttcaccgcc gcgaaaattg
     1621 cttctccaac tcttccgccg tcatccgcag catcgtcggc cggccgtggc cgcaggccag
     1681 cggcgacgag caggcttcca gctggctgac cagctcgcgc atctccagca atgagagcgc
     1741 ctggccggcc cggattgacg tgtggcaggc cgcgctgatc gccagtgact ccagcctgct
     1801 cgtcccgcgt ccgccaccac ccatctcgtc cagcatcttc gccagcacct tcgccggttc
     1861 gcggatcttg aggacggccg gcaccgaacg gatcagaacg ctgcctcccc cgaactcctc
     1921 aatctcgaaa ccgaccgctg ccaactcgtc gcggcaggcg tcgaacgtct cccagtcgcg
     1981 cgccgccagt tgcatctgga ccggctgaag catcagctgc cggtccgacg cacgtagttc
     2041 gaagcctggc atcagcttct cgaacagaat ccgctcgtgg gccgcatgct ggtcgatcag
     2101 atacatgccg tccggaccct cggcgatgat gaacatcgac gcgacctgcc cgagtacccg
     2161 caggaccggc agccgcctgc cgccaaccgg cgcggctccg aactcgaagt cctgttcgga
     2221 tactggttgt gtcggtcggt cgccggtcgg ttcgaggtgc cgctccggat gggcaagcga
     2281 gaatcggcgc tggaccgtcc actcgtccag cggcgacgat accagcgtcg ggatgactgc
     2341 cggtgccgca tggccgacga tcgcctcccg ggttgcgcgc tgcacgagag cgaacgctaa
     2401 tcgctcgtcg gcgaagcgca cttcagcttt cgtcggatgg acgttgacat cgatccggtc
     2461 ggcggggacg cggatctgaa tgcagccgat cgggtgtcgc ccgaccatca gcaacgtgtg
     2521 atacgcctgc tcgaaggccg tccccaactg gcgcgatgtg atcagcctcc cctgggcgaa
     2581 gagatactgc tgctgtcgcg tcgcccggtc gaggtctgga agcgagacga taccactgac
     2641 ctcgaacccg tccggtgtct cgtcgggatt caccagtggc accaacgacc cagcgatctc
     2701 cggcccccag acggcgaccg ccgctccgat cgtgtcaccg ctgccgtccg tcacgaagct
     2761 gcgacggccg tcgatctcca gcgagaacgc gatctgcgga taggcgagcg cgtaggcaga
     2821 cacgatccgc gcaacgtaat ttgcctcggt ctgcggttgg cgcaggaact tgcgtcgcgc
     2881 cggcaggttc gcgaacaggt cgcgggcgac gatcgacgtt ccgccgcccc aggcaacccg
     2941 ctgcggtggt ctgtcgtgac cgaactccac ccgcagcatc ccgcccgccg tctcgccctc
     3001 gtccagcgac tggatccgca gatcggagat cgccgacagc gatgccagcg cctccccacg
     3061 gaagccgagc gtcgtcagcg ccttcaggtc gtcgatggaa gcgatcttgg atgtcgcgtg
     3121 gcgctcgatc gccagcggca gatcgtcgaa cgcgatccca cgtccgtcgt cgcgtacctc
     3181 cagctcgtcg ataccgccac cggcaatcgc gatgtcgatt cgccgtcccc cggcgtcgat
     3241 tgcgttctcg agcagctcct tgacggcagc cgccggccgt tcgatcacct cgccggcggc
     3301 gatcttccca accgtctcca gtgtcagccg ctggatgctc acgccctcgt cgcctcgatc
     3361 gctgttccta gaaaccgcca gggcagatca acgccacgcg tgatgccaat cctcggcgtc
     3421 gccacgatcg tcagcgctac gcgcgccgcc gtgatcccaa accgctcacc gcagaccaac
     3481 gcgccgtggt cgcccgagtc gattcccatc gcgcgcgtca atcgacccgg cccactcgtg
     3541 acgcgctgtt cgccttcgat ctgcaccgcg cgaagcagaa ctgccgacgg ctcaccctcc
     3601 ggtccggtca cgatattgaa gcagggatag accccgtatg ccgaatagac gtagaccatc
     3661 cctggtggtc caaccatcgc cgccgcccgc ccaccgggcc ggaaggcagc atgcgacgct
     3721 ggatcctcaa cgccgccgta cgcttcggtc tcgacgatca gcccagctat ctccaccgcg
     3781 ccgcgccgaa cgaccagcga acggccgatg atgtcgtggg cgatacccgt gacctcacca
     3841 gcgaacgcat cgcaacgcag ataccgactg ctaaccaaga tcggcaagcc gcgcctgaag
     3901 caacgccagc cgctcctcgg cgccggccag cttggcgcgg tggccctcga cgacgttggc
     3961 gggcgcacgg ctgacgaact gttcgttggc cagcatcgcc gtcgcccggc tgatctcacc
     4021 ctcgacctgt tcgatctcgc gcgtgatccg cgcccgctcc gcttccacgt cgatcatgcc
     4081 cttgagtggc aggaaaatcg acgacacacc ggacacgatc gcgacgctcc cttcctgcgc
     4141 ctccggctca ccggcccgat actccagtcc atcggcgtcc agccgcgcca acgagatgag
     4201 ctcgccccgc agcgcatcga acgcctcggt tgccgggccc ggatagacga tggccggaat
     4261 ccggcgagcg gtgtcgacac cctgctcggc ccgggcgttt cggatcagcc gcacggcctc
     4321 cgcgatggcg gcgaacgcct ccgcttcgga cgcgaagcgc tgccccgcct ccggccaggg
     4381 cgcgacgatc agcgcttcac ccggatgcgg cagatgctgc cacagttcct ccgtcacgaa
     4441 cggcatgaac gggtgcagca gccgcagcgt ccgctccagc acatacgcca gcgtctgcgg
     4501 cacggccggg tccggcgtat cggcatacag ccgcaccttg gctgcctcga tgtaccagtc
     4561 gcagaactcc gaccagatga actcgtagag cgaccgcccc gcctcgtgga gctggtactc
     4621 gtcaacctgc cgcgtcacct cggcgatcgt gtgatgcagc cggtcgatga tccaggcgtc
     4681 cgcgaccgcc agcgcgtcag cgtctggcat caacggcgcc ccatcggcgt cccgctcgat
     4741 ctccgccccc tcgatcgccc gcagcgcgaa cttggtcgcg ttgaagagct tgttggcgaa
     4801 gttgcgcatc gactcgaccc gctcattcga catcttcagg tcgttgccgg ggccggaagc
     4861 cgtgatcagc gcgaagcgca gcgcgtcact gccgtactcg gccgtcgcca gcgtcgggtc
     4921 gagcacattg cccttcgtct tgctcatccg ctggccgtct gcgtcacgga ccgtgccatg
     4981 caggtagaca tccctgaacg gcgcttcgcc catgaactcg atcccgaaga agatcatccg
     5041 cgcaacccag tggaagatga tgtcgtagcc cgtctccatc acgtggccgg ggtagaagta
     5101 ctccaggtcc ggcgtctgat ccggccagcc gagcgtcgag aacgtccaga ggcccgacga
     5161 gaaccaggtg tcgagcacat ccgggtcttg gcggatctgg tccgagccgc agtgaccgca
     5221 ggccgtcagc acttcctgat cggtcgccgt cacttccccg caggtatcgc agtaccagat
     5281 cggaatccgg tggccccacc agagctggcg cgagatgcac cagtcgtgga tgttctccat
     5341 ccagttcagg tagacactgc tgaaccggtc ggggatgaag cggaccgtgc catccttgac
     5401 gacctcgatg gcgggctttg ccagcggctc catgctgaca aaccactgct tcgagatcat
     5461 cggctcaacg accgagccgc aacggtcaca atgaccaacc gagtgggtgt gcggctcgat
     5521 ccgcaccagt gcgccgtctt cctcgaggcg cttgaccacg ttcttacgcg cttcggcgat
     5581 cgtctgcccg gcgaacggcc ccgcctcggc gttcatcgtc ccgtcgagat tcatgatgtt
     5641 gatggccggt agtccagctc tgcgtccaat ctcgaaatcg ttcgggtcat gcgccggcgt
     5701 cactttgacg gcaccgctcc cgaactcggg atcgacgatt gcgtcggcca cgatcggaat
     5761 ccggcgaccg acgatcggca ggatcagctc cttgccgacg aggtccttgt agcgctcgtc
     5821 gtccggatga accgccacac cggtgtcgcc cagcatcgtc tccggccgtg tcgtcgccac
     5881 ctcgacggcg ccgctgccgt ccgccagcgg atagcgcagg tgccagagga acgactgctc
     5941 gtcctcatgc acgacttcga ggtccgacag cgccgtcatg cagcgcgggc accatgagat
     6001 gatccgctcg ccgcgataga tcaatccctt gtcatacagg tgcttgaacg ccacgcgaac
     6061 ggcacgcgct ggaccggggt ccatcgtgaa gacgtagcgt gaccagtcgg cggacgcgcc
     6121 cagcaggcgc aactgtccct tgatccgctc ctggtacgtc tccatccagt cccaaatccg
     6181 cgccacgaac tcctcgcggc ccaggtcgtg gcgcgacttg ccctcgcggg ccagctcacg
     6241 ctcgaccacc cactgcccgg caatcccagc gtggtcggcc cccggtagcc agagcgcggc
     6301 atgcccctgc atccgcttgt agcgcgtcag gatatcctcg acggcgctga agagggcatg
     6361 ccccatatgg agctcgccgg tcacattcgg cggcggcatg acgatcacga acggaccccg
     6421 gccccggtca acgggcttga agtagccccc cgattgccag aactgatacc aatgctgctc
     6481 ggccgccgcc ggttcgtagg ccttgccaag ctcgcgtgcc cggttggatg atttctgcct
     6541 ggctgccatt gcttgctcct tcgacgaatg ctcgttagtt attgcgttcg ttgctgcaac
     6601 gggtaaaacc aaaagcccgt cctgacaagg acgggctcac ccgcggtacc acctcgattc
     6661 ggcagcagac gctgccgcac tcttccgcga taacgggcga accccgcggg cgcttactcg
     6721 ctcgttcagc acccggccct ccggacgact tcagccgcgc ctgcgtgccg gggctttcag
     6781 tcagcgaccc cgactccctg tccgccgcga gcggcctact ccttccggag attggcggtc
     6841 tcagcgatgt ttcaatcctc agcgcaacca ccatcgtggt gcgactctgg cgtataattg
     6901 taggctgcgt ccggccctgc gtcaatcagt ggctggcacc tgtcatgcat gtgacaatca
     6961 gtcacggatt ggtactatcg caacctccgt ttgactgcta caatctgcat gcgaatagat
     7021 gttctacgta cggacaccag ccgtaacccc gctgatggac cacgtttgca aagaccggag
     7081 acgagatgcc catcgatcga atcgtcgtcc gtggcgcccg cgagcacaac ctcaagaaca
     7141 ttgatgtcga gatcccacgc gacaagctgg t
//
LOCUS       DASUGJ010000711         5818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_811793, whole genome shotgun sequence.
ACCESSION   DASUGJ010000711 DASUGJ010000000
VERSION     DASUGJ010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811793"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFV93_16125"
     CDS             complement(<1..253)
                     /locus_tag="VFV93_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166934.1"
                     /translation="MTGAAAPHWHGYYDADEIDEICRALNSTTVRSNDIDLRVRVYEQ
                     SGDAPTVMVAHGLLGYGLTFARFHLPFWRRGWRVVQFDFP"
     gene            complement(250..852)
                     /locus_tag="VFV93_16130"
     CDS             complement(250..852)
                     /locus_tag="VFV93_16130"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5166935.1"
                     /translation="MAQTQTIPETHDRTESNRQLAYRFYDQLWNKGNYAAAPELLHPD
                     HFDHSLPPIPDLQQGREGVLQLMQTFREGFPDLVMSVEMVICEGDLVGEVLMLRGTHT
                     GTFAGIPPTGKPIEIRSVNICRIEDGLVRERWGASDDLGLLRQIGVLPSPGTRAWNAS
                     IAAAGAQIRLRTHGRKLAGATAAGVAAIAALALRRRGSRG"
     gene            complement(1007..1234)
                     /locus_tag="VFV93_16135"
     CDS             complement(1007..1234)
                     /locus_tag="VFV93_16135"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX5166936.1"
                     /translation="MATGIIKSMLRDKGFGFIAPDNAQGDGSDLFFHQSSVADYGFDM
                     LEEGQRVEFTEEPDPRNASRRRAANVSLAAE"
     gene            1535..2275
                     /locus_tag="VFV93_16140"
     CDS             1535..2275
                     /locus_tag="VFV93_16140"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX5166937.1"
                     /translation="MYYDQLGFDARLEWGIEGVLRLGPRSDVLVIVDVLSFSTAVDIA
                     VGRGARVLPFRWRDERAIGYARDAGALLAGSMRQPTVDEPYTLSPSSLTTIPPGTLLV
                     LPSPNGSSLSKTAADLGTAVFAGCLRNATAVATAARSVGSVVTVIAAGERWDDGESLR
                     PAVEDLLGAGAILSALDATAPSPEAQAAMAAFAAAVPDLARQLSESASGRELIERGFA
                     ADVALAAELGVSRSAPFLRDHAFIDLAV"
     gene            complement(2289..5057)
                     /locus_tag="VFV93_16145"
     CDS             complement(2289..5057)
                     /locus_tag="VFV93_16145"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX5166938.1"
                     /translation="MPEQAGQDATRVFVSYASVDRDRVMPIVRALQDIGVDVWIDQND
                     IAGGTLYAAEIADGIQGCAALVLMCSVASLASHNVRQELALAWKYRRPYLPLLLEPVT
                     IPRELEYWLEGSQWIELLDQPADVWLPKLRQAIERLELGMSTPGATPQPAVERTTSTL
                     FDVPVPLAPLVGRRETIDAVIRLIDSTRLVTLTGAGGSGKTRLALAAAEAIRDRFAGD
                     IVFVDLAPIRDPELVLPTIATRLGIQTMGDQPILTAMTQALARRELLLILDNLEQVID
                     AAPQIAGLLAVATKLRVLATSRSPLQLAGEQEYPVVPLSVPDLANESDLEEMARADAV
                     QLFALRAQLIRPEFQVSEDNVAAIAEICVRLDGLPLAIELAASRLRMMSPQALLDRLD
                     RRLPLLAGGRRDLPDRQQTLRSTIDWSHELLQPDEQALLRRLSVFAGGWTLEAADAVA
                     GGAIDVFSGLEALVSQSLVRVNDAADPRYTMLETIREYGLDQLVGAGEAETVHERHAT
                     WVLQMVETIAPQTRGPRLVDAMNQLEAEHANILVAIDWFIDRRNATSGWRIAGPLWLF
                     WWMRNHLRVAHQHIQRLLALPREGVPVETLARGLTCAGIVNEWLNLADVAARLQTDAL
                     EIWERVPFDFRTDEYWSHACLSNLAMRSGDLERAREQNDRTVEIARERNDEFLLAMAS
                     VNPGVHASLAGKFAEAASIYREALTHARRSGDPWAISLILGNLISAVLELDGQADIGA
                     DLDELITLNTALGHTYHLILSFGLRGTVALREGRPQDALADIQEARVLARRFGNAYTE
                     AAELNDIGRIYLLLDRVDEARETYRDSLSLGREYSMPATCPAALEGIARILHISGRPA
                     SAIRIVAAADAIRATHGPVLAGTDREAIEQLLPALRSALGDSVYAAEWVAGAAMTMDE
                     AIAYALAP"
     gene            5188..>5818
                     /locus_tag="VFV93_16150"
     CDS             5188..>5818
                     /locus_tag="VFV93_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5166939.1"
                     /translation="MALGQHQPLWWQAGVVYQIYPRSFRDSNRDGVGDLAGVIEKLDY
                     VSETLGVDAIWLSPFYPSPMADFGYDVSDYCAVDPLFGDLATFDRLVAEAHARGLRVI
                     IDYGPNHTSDQHPWFIESRSSRDSPKRDWYVWADAKPDGTPPNNWFSIFGGSAWQWDD
                     ATGQYYLHSFLVEQPDLNWHNPDVRAAMLDAMRFWLARGVDGFRLDAIHC"
BASE COUNT          973 a         1864 c         1885 g         1096 t
ORIGIN      
        1 cggggaagtc gaactggacg acacgccagc cgcgccgcca gaacggcagg tgaaagcgcg
       61 cgaaggtcag gccgtagccc agcaggccgt gggcgaccat cacggttggc gcgtcgccgg
      121 actgctcgta gacacgcacg cgcaggtcga tgtcgttgct gcgtaccgtc gtgctgttca
      181 gggcgcggca gatctcgtcg atctcatccg cgtcgtagta gccgtgccag tgcggggccg
      241 ccgctccagt cacccccggc tgccccggcg gcgcagcgcg agcgcggcga tcgcggcgac
      301 gccggccgct gtcgccccgg cgagcttcct gccatgcgtg cgcagccgga tctgggcgcc
      361 ggccgctgcg atcgacgcgt tccaggcgcg cgtgccgggc gatggcagca ccccgatctg
      421 gcgcagcagg ccgaggtcgt cggatgcgcc ccagcgctcg cgaaccagcc cgtcctcaat
      481 ccggcagatg ttgacgctcc tgatctcgat cggcttgccg gtcggcggga tgccggcaaa
      541 cgtgccggtg tgggtcccgc gtagcatcag cacctcgccg accaggtcgc cctcgcagat
      601 gaccatctcg accgacatca ccaggtccgg gaatccttcg cggaaggtct gcatcagttg
      661 caggacaccc tctcggccct gttgcaggtc agggatcggc ggcaggctgt ggtcgaagtg
      721 atccgggtgc aggagttccg gcgcggcggc gtagttgccc ttgttccaga gctgatcgta
      781 aaaccggtag gccagttgcc ggttggactc tgttcggtcg tgagtttcgg gtattgtctg
      841 ggtctgtgcc atgcatgcct ccttgagtgc aggtgtcgga tcgttccgag gcttcgcgat
      901 gaccgttctc cgaggtaatc ggcagtatag cagtggcaac ggcaagcgcc ccggaacgtg
      961 ttccggggcg ctcgtttgcg tcttgtggtg tcagactggc cggatgttac tccgctgcca
     1021 gcgagacgtt cgccgcgcgc cggcggctgg cgttgcgcgg atccggctct tcggtgaact
     1081 caacgcgctg accctcttcg agcatgtcga agccgtagtc agccacgctg gactggtgga
     1141 agaagagatc cgagccatcg ccctgcgcgt tgtcaggcgc gatgaagccg aagcccttgt
     1201 cgcgcagcat tgacttgatt attccggttg ccatctggca attcctttcc ttcgtcggtg
     1261 acgtccggtc cggaagcaca gataccccgg ccaggccaca acaacggccc cagccgagct
     1321 tgcttactca ctctgcctgc cacgttgagc gtgcaggatc gcgatcccgc tgcgcacgac
     1381 aggcattgtc atacctgagg tacgtcaagc atatcaaaca ggttagctcg acgtgattct
     1441 tctcaattgg catgcgttcc atgtcgggat gacgcttgta gcgggcagta tcgtttcatg
     1501 acgattggaa gcgattcagc ataggacggt gcggatgtat tacgaccagc ttggttttga
     1561 cgcgcgcctt gagtggggca tcgaaggtgt tctgcgattg ggaccgcgct ctgacgtgct
     1621 ggtgatcgtc gatgtcctct ccttctcgac ggcggtcgac atcgcagtcg ggcgcggcgc
     1681 gcgggtgctg ccctttcgct ggcgtgacga gcgcgctatc ggctacgcgc gggacgccgg
     1741 agcgctgctg gcgggcagca tgcggcagcc gactgtcgac gagccgtata cactctcgcc
     1801 gtcgtcgctg acgacgatcc cgcccggcac gctgctggtc ctgccgtcgc cgaacggctc
     1861 ttcgctctcg aagaccgccg ccgacctggg cacggccgtc ttcgccggtt gcttacggaa
     1921 tgccacggcg gtcgcgacgg cggcgcgctc ggttgggagc gtcgtgacgg tgatcgccgc
     1981 cggtgaacgc tgggatgacg gcgagtcgtt gcgcccggcc gtcgaagacc tgctcggcgc
     2041 cggcgcgatt ctcagcgcgc tcgacgccac tgccccgtca ccagaggcgc aggccgcgat
     2101 ggcagcgttc gcggctgcgg ttcccgatct cgctcggcaa ctatccgaat cagcgtccgg
     2161 ccgggaactg atcgagcgcg gcttcgccgc cgatgtcgct ctcgccgctg aactgggtgt
     2221 cagccggtct gcgcctttcc tgcgagacca tgcgttcatt gatctggcag tttgaaatgg
     2281 caacgaggtc atggcgcaag cgcgtatgcg atcgcttcat ccatcgtcat tgccgcacct
     2341 gccacccact cggctgcgta cactgagtca cccaacgctg aacgaagtgc cgggagaagt
     2401 tgctcgatgg cttcgcggtc tgtgcccgca agcacgggtc cgtgggtggc acgaatcgca
     2461 tctgctgccg caacgatgcg gatagcggaa gccggacgcc cggagatgtg caggatccgc
     2521 gcgatgcctt cgagcgccgc tgggcaggtc gccggcatgc tgtattcacg tcccaggctg
     2581 aggctgtccc gataggtttc gcgagcctca tcaacccggt cgagaagcag gtaaatgcgg
     2641 ccgatgtcgt tgagttcggc ggcttcggta taggcattcc cgaaccgcct ggcgaggacg
     2701 cgtgcttcct gaatgtccgc gagcgcgtcc tgtggccggc cctcgcgtaa cgcgaccgtc
     2761 cctcgcagcc cgaacgacaa gatcaggtga tacgtgtgtc ccaaggcagt gttcagcgtg
     2821 atgagctcgt caaggtccgc gccgatatcg gcctggccgt ccagctccag caccgccgag
     2881 atgagattgc cgaggatgag cgagatcgcc cacggatctc cggatcgccg ggcgtgtgtc
     2941 aatgcctccc ggtagatcga tgctgcttca gcgaatttac ctgcaagtga agcgtggact
     3001 ccgggattaa ccgatgccat cgccagcagg aattcatcgt ttctctcgcg ggcgatttct
     3061 accgtgcggt cattctgttc cctcgctcgt tcaagatctc cactgcgcat ggccaggttg
     3121 gacaggcagg catgcgacca gtattcgtct gtcctgaagt cgaacgggac gcgctcccag
     3181 atctccaacg cgtccgtttg caggcgggcc gcgacatcag caaggttgag ccactcgttg
     3241 acaataccgg cgcaggtgag cccgcgcgcg agtgtttcca caggcacacc ttctcgtgga
     3301 agggcaagca accgctgaat gtgctggtgt gcgactcgca ggtggtttcg catccaccag
     3361 aatagccaga gcggccccgc gattcgccag ccggacgtgg cgttccgccg atcgatgaac
     3421 cagtcgatgg cgaccagaat gttggcatgc tcggcttcaa gttgattcat cgcatcgacg
     3481 agccgtggac ccctcgtctg tggcgcgatc gtctccacca tctgcaggac ccaggtcgcg
     3541 tgtcgctcat gcaccgtctc ggcttccccc gcaccgacaa gctggtccag gccgtactcg
     3601 cggatcgtct ccagcatcgt gtagcgtgga tccgcggcgt cgttgacgcg aacgaggctc
     3661 tggctgacca gtgcttccag cccgctgaac acatcgatcg cgccgccggc gaccgcgtcg
     3721 gcggcttcca acgtccagcc tccagcgaac acgctgaggc ggcggaggag tgcctgttca
     3781 tcgggctgca gcagctcatg gctccagtcg atcgtgctgc gcagcgtctg ctgccggtcc
     3841 ggcaggtctc gccggccacc ggccagcagc ggcaggcgtc gatcgaggcg gtccagcagc
     3901 gcctgtggcg acatcatccg caggcgtgag gcggccaact cgatcgccaa cggcagtcca
     3961 tccagccgga cacagatctc agcgatcgcg gcgacgttgt cctccgagac ctgaaactcc
     4021 ggtcggatca attgcgcccg tagagcgaac agttggacgg cgtcggcccg ggccatttcc
     4081 tccagatcgg attcgttcgc cagatcaggc acagacagcg gtacgacagg atattcctgc
     4141 tcaccggcaa gttgcagcgg ggagcggctc gtggccagta cacgcagctt ggtggcgacc
     4201 gccagtagtc ctgctatctg tggcgcagcg tcgatgacct gctccagatt gtcgaggatc
     4261 agtaacaact cgcggcgagc cagcgcctgg gtcatcgccg tcaggatcgg ctggtcaccc
     4321 atcgtctgaa tgccgagccg ggtggcgatc gttggtaaaa cgagttcggg gtctcgaatc
     4381 ggcgcgagat cgacgaagac aatatcgccg gcgaaccggt cacggatcgc ctcggcggca
     4441 gccagcgcca ggcgggtctt ccccgagcca cctgctcccg tcagcgtgac gagccgcgtc
     4501 gaatcaatga gcctgataac cgcatcgatc gtctctcgcc ggccgacgag cggcgcgagt
     4561 ggaacgggga catcgaacag cgtcgatgtc gttcgctcaa cggcaggttg tggcgtggct
     4621 ccgggagtcg acatgccgag ttccagccgc tcgatcgcct gccgcagctt cggcaaccag
     4681 acgtcggccg gctggtcgag tagctcgatc cattgcgagc cttcgagcca gtattcgagc
     4741 tcgcgtggga ttgtgaccgg ctccagcagc agcggcaggt acggacggcg atacttccag
     4801 gcaagcgcta gttcctgccg aacattgtgg gatgcgagcg acgcgactga gcacatcagt
     4861 accagggcag cgcagccttg gatgccatcc gcaatctcag ccgcgtagag cgttccgccg
     4921 gcgatgtcgt tctgatcgat ccagacatcg acgccgatgt cctggagagc tcggacaatc
     4981 ggcatcacac gatcgcgatc gacgctcgcg taggagacga agacacgggt tgcgtcctgc
     5041 ccagcctgtt cgggcaccgg atgactcctc tgtcgcggac aaatctggtg ctgttagcat
     5101 agtctcgata tcgttcgcac acaacccatc gcgatacact cacgaatcgt gcgggtcgca
     5161 agcgccggcg agagagggtg gtgcgcggtg gcgttgggac aacatcagcc gctctggtgg
     5221 caggcggggg tcgtctatca gatctatccg cgcagctttc gcgacagcaa tcgtgatggt
     5281 gtcggcgatc tggccggggt gatcgagaag ctggattacg tcagcgagac gcttggcgtt
     5341 gacgcgatct ggctctcacc cttctacccc tcgccgatgg ccgactttgg ctacgatgtc
     5401 tccgactact gcgcggtcga tccgctcttc ggcgatctgg cgacgttcga ccggctcgtt
     5461 gccgaagcgc acgcccgcgg cctgcgggtc atcatcgact acggtccgaa ccacacctcc
     5521 gaccagcacc catggttcat cgagtcgcgc tcctcgcgcg acagcccgaa gcgcgactgg
     5581 tacgtctggg ccgatgcgaa gccggacggg acgccgccga acaactggtt ctcgatcttc
     5641 ggcggctcgg cctggcagtg ggacgacgcc accggccagt attacctgca ctcgttcctg
     5701 gtcgagcagc ccgatctgaa ctggcacaac cccgacgttc gggccgcaat gctggacgcg
     5761 atgcgcttct ggctggcgcg cggtgtcgat ggcttccggc tcgacgcgat ccactgca
//
LOCUS       DASUGJ010000712         3760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1505729, whole genome shotgun sequence.
ACCESSION   DASUGJ010000712 DASUGJ010000000
VERSION     DASUGJ010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505729"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="VFV93_16155"
     CDS             <1..1767
                     /locus_tag="VFV93_16155"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX5166940.1"
                     /translation="ISAQPLTELVPVQPAAMEGRYICQWDKDSCDDASFVKIDFLALG
                     MLSMVEECLELIVRQGKPPPDLSRIDFEDRDVYDMIDRGDTIGVFQIESRAQIQMLPR
                     TKPKNLEDLTVQVAIVRPGPITGGAVTPYVEARRELREGRNVQPGYDHPLLEPVLRET
                     HGAILFQEQVLEVAVQLAGFTHGQADSFRRSMSRKRSLEAMDGFWQQFREGASQRGVD
                     EETARIVFDKLLGFAQYGFPKSHAAAFAVLAYQSCWLRYYHPAPFYCALFNNQPMGFY
                     PPHAFANDAQRHGTPIYPPDVNLSGAKCTVEGHGVRIGFNYVDGLGEDVAEALVAERE
                     RAGPYRSLIDLARRSRLRRDAIDHLIVVGACDGFGLRRREMLWQLGLFMPDKRLGRKN
                     QPADRQLALDLPTEQDMVTLREMTDWERMIADYDILGLSSRYHPLALLRPILPRGLAR
                     AADLEQLRNGSVVQVGGLVVCRQRPGTAKGMLFMLLEDETGLANVIVHPPLYEKRRNV
                     IRGNPFLLVVGRLQLREETVNIIASDIRAIERPSASGPQPTQSISDLLDQIDRLESQP
                     DEINEEQLTSLRLAAPAGHDFR"
     gene            1821..2054
                     /locus_tag="VFV93_16160"
     CDS             1821..2054
                     /locus_tag="VFV93_16160"
                     /inference="COORDINATES: protein motif:HMM:NF021558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HEX5166941.1"
                     /translation="MIRTTSLEAKTKLDDLIDAALRGEDVLITAVDERGERTVRIVAI
                     SDREPRRKPGSLKGKIWMSDDFDEPLDDFKDYQ"
     gene            2057..2443
                     /locus_tag="VFV93_16165"
     CDS             2057..2443
                     /locus_tag="VFV93_16165"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5166942.1"
                     /translation="MRALLDTHTLLWFFEDSPRLSARVTQLLAAASTDAVISIASLWE
                     IAIKMSVGKLTINYAFDDLIATQLPQNDIVLLSVSAQHVAGVVDLPLHHRDPFDRMLV
                     AQSVAEYLPIISADPIFDVYRVQRIW"
     gene            complement(2456..2788)
                     /locus_tag="VFV93_16170"
     CDS             complement(2456..2788)
                     /locus_tag="VFV93_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166943.1"
                     /translation="MRKFPIVVAISGGPLFSLQKTCRYCPPCDLLILHKDELEAQLAI
                     AVPEHGPHAAGNPYTVVGTMTRADWKRMRDGVTDPREFTNAVAIFEQIYNLKFRPAHW
                     APDNEQDR"
     gene            complement(2896..3483)
                     /locus_tag="VFV93_16175"
     CDS             complement(2896..3483)
                     /locus_tag="VFV93_16175"
                     /inference="COORDINATES: protein motif:HMM:NF015629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HEX5166944.1"
                     /translation="MGDEHSGVDLRTLPVDLSELLLAAESHDAEMRTFIDRETGELVL
                     VFETSFQIADEYVTSQSEAIEVSSELAEQLVDDNQLPAWQIDELADALKVALDSSGRY
                     VPVEPRWSSEGFRDMQDFAWSLDDPTHRQHLERALGGKGSFRRFKNALDDLPKELRGE
                     WFAFRDARAREDIVDWLRSIGIEPIDTPRHQKESK"
     gene            3559..>3760
                     /locus_tag="VFV93_16180"
     CDS             3559..>3760
                     /locus_tag="VFV93_16180"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5166945.1"
                     /translation="MQIRGITEIVLNVHDKQAALAFYRDLLGLEVISRPEMPNVFLKA
                     GPGQAGVPQMIVLVPLPDGADTF"
BASE COUNT          717 a         1137 c         1181 g          725 t
ORIGIN      
        1 atctccgcgc aaccgctgac ggagctcgtg ccggtccagc cggccgcgat ggaggggcgc
       61 tacatctgcc agtgggacaa ggattcctgc gacgacgcga gcttcgtcaa gatcgacttc
      121 ctggcgttgg ggatgctctc gatggtggag gagtgtctgg agctgatcgt ccgccagggc
      181 aagccgccac ccgacctgtc gcggattgac ttcgaggacc gcgatgtcta cgacatgatc
      241 gaccggggcg atacgatcgg tgtcttccag atcgagagcc gggcgcagat tcagatgttg
      301 ccgcggacga agccgaagaa cctggaggac ctgaccgttc aggtagcgat cgtccgaccg
      361 ggaccgatca ccggtggggc cgtgactccc tacgtcgagg cgcggcgcga actccgcgag
      421 ggcagaaacg tccagcccgg ctacgaccat ccgctgctgg agccggtgct gcgcgagacc
      481 catggcgcga tcctgttcca ggagcaggtg cttgaggtgg ccgttcagtt ggcgggtttc
      541 acgcatggtc aggccgattc cttccgtcgc tcgatgagcc gcaagcgctc gttggaggcg
      601 atggacggct tctggcagca gttccgcgaa ggcgccagcc agcgcggtgt cgatgaagag
      661 acagcccgga tcgtcttcga caagctgctc gggttcgcgc agtacggctt cccgaagtcg
      721 cacgcggccg ccttcgccgt tctcgcctac cagtcgtgct ggctgaggta ttaccacccg
      781 gccccgttct actgtgcgct attcaacaac cagccgatgg gcttctaccc gccgcatgcc
      841 ttcgccaacg acgcccagcg gcacggcacg ccgatctacc cgccggatgt caacctgagc
      901 ggcgcgaagt gcaccgtcga ggggcatggc gtgcggatcg gctttaacta cgtcgatggc
      961 ctcggcgagg acgtcgccga agcgctcgtt gccgagcggg agcgggctgg gccgtatcgc
     1021 tcgctgatcg acctcgcccg gcggtcacgg ctgcggcgtg atgcgatcga tcacctgatc
     1081 gtcgttggcg cctgcgatgg gttcgggctg cggcgtcgcg agatgctctg gcaactcggt
     1141 ctgttcatgc cggacaagcg gcttggcagg aagaaccagc ccgctgaccg gcagctcgcg
     1201 cttgacctgc cgacggagca ggacatggtc acgctgcgcg agatgaccga ctgggaacgg
     1261 atgatcgccg actacgacat tcttggcctc tcgtcgcgct atcacccgct ggcgctgctg
     1321 cggccgatcc tgccacgcgg attggcgcgg gccgccgatc tggagcagtt acgtaatggc
     1381 agtgtcgtcc aggtcggcgg gctggttgtc tgccgccagc ggccagggac ggccaagggc
     1441 atgctcttca tgctgcttga ggacgagacc gggctggcga acgtcatcgt ccacccgcca
     1501 ctctacgaga agcgccgcaa tgtcatccgt ggcaacccgt tcctgctggt ggttggccgg
     1561 ctgcaactgc gcgaggagac ggtcaacatc atcgcgtccg acatccgcgc catcgagcga
     1621 ccgagtgcgt ccggtccgca accgacgcag tcgatctccg acctgctgga ccagatcgac
     1681 cgcctcgaat cgcagcccga cgagatcaac gaggaacagc tcaccagcct gcgcctcgcc
     1741 gccccggccg gccacgactt ccgctgatgt gatgctacta ttgatactga acgtgccaga
     1801 cgagcaacag gagccttgtg atgattcgca cgaccagtct ggaagcaaag acgaagctcg
     1861 atgacctgat cgatgccgct ctacgtggcg aagatgtgct catcacggct gtcgacgaac
     1921 gtggagaaag aaccgttcgg atcgtcgcga tttccgaccg ggagccacgc cggaagccag
     1981 ggagtctcaa gggaaaaatc tggatgtcgg atgacttcga tgaaccactt gatgatttca
     2041 aggactatca atagcgatgc gagcgcttct cgatacgcac acgcttctct ggttcttcga
     2101 agacagtcca cggttgagcg cacgcgtgac gcagctactg gcagccgcaa gtaccgacgc
     2161 ggttatcagc attgccagcc tgtgggaaat cgcgatcaag atgagcgttg gaaagctgac
     2221 gatcaactat gcctttgatg atctgatcgc gacgcaactc ccacagaatg acatcgtgct
     2281 tctcagcgtc agtgctcaac atgttgccgg cgtcgttgat cttcccctcc atcaccgcga
     2341 tccgtttgac cgcatgctgg tagcgcagtc cgtcgcagaa tacctgccga tcatcagcgc
     2401 cgatccgatc ttcgatgtat atcgcgtcca gcgcatctgg tgacgtgcga gccactcaac
     2461 gatcctgctc gttgtctggc gcccaatgag cggggcggaa cttcaggttg tagatctgtt
     2521 caaagatcgc aacggcgtta gtgaactcgc gcgggtcagt cacgccatcg cgcatccgct
     2581 tccagtcggc tcgcgtcatc gtcccgacga ccgtataagg gttgccggcg gcgtgcggtc
     2641 cgtgctccgg taccgcgatg gccagttgcg cttccagttc gtccttatga aggatcagca
     2701 gatcgcatgg cgggcagtac cggcaggtct tttgaagcga gaacagcggc cccccagaga
     2761 ttgcaaccac gatcgggaac ttgcgcacca gcgtcctctg cttgcacagc ggacacttcg
     2821 tcaggcgtgt gtcggtatac gggttcagga tgaacttgta gcggcgcgct ggcggattct
     2881 cccttcgggg caacgtcatt tactctcttt ctggtgacgt ggcgtgtcga tcggctcaat
     2941 cccgatgcta cgcaaccagt caacgatgtc ctcgcgggcg cgagcgtcac ggaaggcgaa
     3001 ccactcaccg cggagttcct tgggcaggtc atccaacgca ttcttgaagc ggcgaaacga
     3061 tcccttacct ccgagcgcac gttccaggtg ttgccggtga gttggatcgt caagcgacca
     3121 ggcgaaatcc tgcatatccc tgaagccctc cgacgaccag cgtggctcga ctggaacata
     3181 acgaccactt gagtcgagcg cgaccttgag tgcatcggcg agttcatcga tctgccaggc
     3241 aggcaactgg ttgtcatcca cgagctgctc ggcaagttcg gaactgacct cgatcgcttc
     3301 ggactgtgac gtaacgtact cgtcggcgat ctggaatgac gtctcaaaaa ccagaacgag
     3361 ttcgcctgtc tcgcgatcga tgaacgtgcg catttcagca tcgtgagact cagcggcgag
     3421 caagagttcc gaaagatcga ctggcagagt gcgaaggtct acgccactgt gttcatcccc
     3481 cacgatcgac tccattcagc tagcatggtg actccgccag aactgggagc ggtgatgatg
     3541 acatcgagga gaacggcaat gcagatacgg ggcatcacgg agatcgtgct gaacgtccat
     3601 gacaagcagg cggcgttggc attctatcgt gatctgctgg ggctggaggt tatctcgcgg
     3661 ccggagatgc cgaatgtgtt cctgaaggcg gggccggggc aggcgggggt gccgcagatg
     3721 attgtgctgg tgccgctgcc ggatggcgca gatacgtttg
//
LOCUS       DASUGJ010000713         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1367890, whole genome shotgun sequence.
ACCESSION   DASUGJ010000713 DASUGJ010000000
VERSION     DASUGJ010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367890"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..2714
                     /gene="mrdA"
                     /locus_tag="VFV93_16185"
     CDS             306..2714
                     /gene="mrdA"
                     /locus_tag="VFV93_16185"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5166946.1"
                     /translation="MSRPKQPRGSAPLSSRPSRGSTGSNRRVNRPSGNRRSYGPGGTW
                     SGSSKWSPYDRRERLWPKVLLIVSVVGVLIFGSILVASRLTGLNGDDDDNQAALASPV
                     GDETPTAEQTAGGLADETPVIEAPDPNATVGASPTAVVDVGEVKAAKDVAEAYAKVWE
                     AGNYDQLYDLISRDSQAFISREDFVARYEGIAVEVGIVTLKAEITGGADDDELFPMKV
                     TIDSSRVGTIEDENLLPVINDGDKPAIDWSPSLIFSQLGDGYVQWTSQIPQRGRILDR
                     KGRPLAQMGQITRVGVVPGKITNEADLLQKLSQLLDMPQDVIKNRYEGGQADWFMAVK
                     DLPGDVDQSLIDQLNAIEGVAIQKPPARVYPQGELAAHVVGYMSEVTAEELPELSKQG
                     YEAGDLVGRTGIEASAEKWLAGKRGGTLALIGPDGGTIRVLGEVPMEDAHDVVLTLDI
                     DLQRAAFDALGDQQGATVVLDPNNGEVLAMVSKPSFDPNQFILGVTDEQWTQLMDPQR
                     QPLLNRAVAFATSTGSIFKVITASAGMVHMGLTPDSDTPCPGEFRLEGVDQVWHDWVP
                     GGQGDLNLHNGIVRSCNTVFYRMGAELDAMDEHFLPDMAKAYGLGKLTGLPELYEIPG
                     IVPDPEWKMENVGDFWARGDAINLAIGQGYLTATPLQMANVYAAIANGGTLYQPHLLL
                     DVVKLDGSIVQSGEVKENGKLPLSADQISWLQDALYDVVNADNGTAVEPFIGIQHPVS
                     GKTGTEETGTEAQGTNAWFASYTPSDDPKMANIVFVEKGVAGSKAAAPVSREIIDAWY
                     TLYP"
BASE COUNT          510 a          938 c          882 g          452 t
ORIGIN      
        1 cccacgtccc gcgtcccgcg tcccgtataa cgccctcccc accccctgac ttgtcatcac
       61 catcgccgct ggcggcatac cgcgccttga ctcgtgagca ccggcgtggg acagtacgta
      121 cagtggtgac gaggccgtgg cacggtgctt ggtgaagacg tgttgtggtc ctcggattcg
      181 cgcgagcagt cattctggcc agcgttggcg tcgtggtcgc gcggtatgcc gatgtccggc
      241 tttcatctgc atgcaacatc gccgtccgcg ctcgtcgcga cggtgccgga aggtagaacg
      301 ccctcgtgtc ccgtccgaaa caaccccgtg gctcagcgcc gttatcatcc cggccatcac
      361 gcggctcgac cggcagcaac cggcgcgtca accgtccctc cggcaatcgc cggagctacg
      421 gccccggcgg gacgtggtcc ggctcgtcga agtggtcgcc gtacgatcgt cgcgagcggc
      481 tctggccgaa agtgctgctg atcgtctcgg tcgtcggcgt tctgatcttc ggctcgattc
      541 tggtcgccag ccggctgacc gggctgaacg gcgatgatga cgacaaccag gccgcgctcg
      601 cctcgccggt cggcgacgag acgccaaccg ccgagcaaac agccggtggg ctagccgacg
      661 agacaccggt catcgaagca ccagatccga acgcaacagt cggcgccagc ccgacggcgg
      721 ttgtcgatgt cggtgaggtc aaggcggcta aggacgtcgc cgaggcgtac gcgaaggtct
      781 gggaggcggg taactacgac cagctctacg acctgatctc gcgtgactcg caggcgttca
      841 tctcgcggga ggatttcgtc gcccgctatg aggggattgc cgtcgaggtc gggatcgtca
      901 ccctcaaggc cgagatcacc ggtggcgctg acgatgacga actcttccca atgaaggtca
      961 ccattgattc atcccgcgtc ggcacgatcg aggacgagaa cctgctgccg gtcatcaacg
     1021 atggcgacaa gcccgctatc gactggtcgc cgtcgttgat cttctcgcaa ctcggcgatg
     1081 gatacgtcca gtggaccagc cagatcccgc aacgcggacg gattctggac cgcaagggac
     1141 ggccactggc gcagatgggc cagatcacgc gggttggcgt cgtgccgggc aagatcacca
     1201 acgaggcaga cctgctccag aagctctcgc aactgctcga catgccgcag gacgtcatca
     1261 agaaccgcta cgagggcggc caggccgatt ggttcatggc tgtcaaggac ctgcccggtg
     1321 atgtcgatca gagcctgatc gaccagctca acgccatcga gggcgtcgcc atccagaagc
     1381 caccggcacg cgtctacccg cagggcgaat tggccgccca tgttgtcggc tacatgagcg
     1441 aggtgacggc tgaggagctg ccagagctct ccaagcaggg ctacgaggcc ggcgacctcg
     1501 tcggccggac cgggatcgaa gcgtcggccg agaagtggct ggccggcaag cgcggcggca
     1561 cgctggcgct gatcggcccc gacggcggca cgatccgcgt cctcggcgag gtgccgatgg
     1621 aggacgccca cgatgtcgtc ctgacgctgg atatcgacct ccagcgggcc gcctttgacg
     1681 cgctcggcga ccagcagggg gcgacggtcg tcctcgaccc gaacaacggc gaagtgctgg
     1741 cgatggtctc gaagccgtcg ttcgatccca atcaattcat cctcggcgtc accgacgagc
     1801 aatggacgca actgatggac ccacagcgcc aaccgctgct caaccgcgcc gtcgcctttg
     1861 ccacgtcgac cggctcgatc ttcaaggtca tcaccgcctc ggccgggatg gttcacatgg
     1921 gactgacacc ggacagcgat acgccctgcc ccggcgagtt ccggctggaa ggtgtcgatc
     1981 aggtctggca cgactgggtg ccgggcggtc agggcgatct gaacctgcac aacgggatcg
     2041 tccgctcctg caacaccgtc ttctaccgga tgggcgccga actggacgcg atggacgagc
     2101 acttcctgcc ggacatggcc aaggcctacg gacttggcaa gctgaccggt ctgccggagc
     2161 tgtacgagat tcccggtatc gtccccgacc ccgagtggaa gatggagaac gtcggcgact
     2221 tctgggcgcg cggcgacgcg atcaacctcg cgatcggcca gggctatctc acggcgaccc
     2281 cgctccagat ggccaacgtc tacgccgcga tcgccaacgg cggcacgctc taccagccgc
     2341 acctgctgct cgatgtcgtc aagctcgacg gatcgatcgt ccagtccggt gaggtcaagg
     2401 agaacggcaa gctaccactc tccgccgacc agatcagttg gctgcaggac gccctctacg
     2461 acgtcgtcaa cgctgacaac ggaacagccg tcgaaccgtt catcggcatc cagcaccccg
     2521 tttccggcaa gaccggcact gaggagaccg gcaccgaagc ccaaggcacc aacgcctggt
     2581 tcgcgtccta cacccccagc gacgacccga agatggccaa catcgtcttc gtcgaaaagg
     2641 gcgtcgccgg ctcgaaagcc gccgcccccg tctcccgcga gatcatcgac gcctggtaca
     2701 cgttgtatcc gtagtgcctc ttcgcgtagg ggcgatggcc gcagcctcgc cctgctccgt
     2761 gcgatcgatg aagggcgagg ct
//
LOCUS       DASUGJ010000714         7101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_952263, whole genome shotgun sequence.
ACCESSION   DASUGJ010000714 DASUGJ010000000
VERSION     DASUGJ010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952263"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..829)
                     /locus_tag="VFV93_16190"
     CDS             complement(23..829)
                     /locus_tag="VFV93_16190"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5166947.1"
                     /translation="MRTLALLRAFLRRDWVNETSYRSMLLIETLGVGSSLILFYAIGL
                     LVGGESEPLQRYGGDYFPFALIGLAVSGYFSISLISASNRLRQAQTTGTLEAMFVTAT
                     PPSALLALSTAWDYLLATVRLAVFVTVGVIAMGVKFDINVWAALPVIVLSLASFASLG
                     LIAASAILVVKRGDSIAILGNIVTTVFGGVLFPISLLPAWARPISYLVPLSWALDGLR
                     RATLVGAGPRDIAGSLLALAISTALLVPLAWLSLRWATDRVRRDGSIGHY"
     gene            complement(826..1815)
                     /locus_tag="VFV93_16195"
     CDS             complement(826..1815)
                     /locus_tag="VFV93_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166948.1"
                     /translation="MTGASIARRPATTRYNSDAVPPAVEGPPMTPAIETNQLTRDFRE
                     PGRLFRRASLEGNRALDGMSFSVEPGECVALVGPNGAGKSTLLRILATVILPTSGTAR
                     VCGYDVASQPKGVRRLAGVMTGDDRSFFWRLTGRENLLFFAELQGLSTTIARQRVNEL
                     LDTLSLSFAADRRFSGYSTGMRQRLGLARALLHHPRVLLLDEPTANLDLEHRGHVMSL
                     LRSVLATGETTAMIASHDAGLAVSLANRVIRLDHGQITTASDEREPQRYLLQVRGLTT
                     EQRQALMMSDDTDTLAIDDLGDGHALAAAVAAIVSGGGEIISVEPAGQFRAVR"
     gene            1844..3031
                     /locus_tag="VFV93_16200"
     CDS             1844..3031
                     /locus_tag="VFV93_16200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5166949.1"
                     /translation="MADYTADAVVIGGGVNGTSAAFQLAKMGMTNVILVERRQLGAGA
                     TGKSGALVRMHYTNTPESQLAFESFKIFKAFQDEVGGDPGWEGPGFVQIVAPGYEEAL
                     ARNVERQQGLGIDTRMVSKEELKEIQPGIYVDDIGAAAYEPQSGFADPNATAYSFAQA
                     AEKLGVKIWTQCEATAIVRDGDKVVGVETERGRIATNTVILAPGAFADRLLKPLGLDY
                     GLYPHRSMVAIFHWPVGWSDRHVVVIDNIHHTWLRPEGTRGTLIGLEQPRQEWDPENF
                     EEGVEGSFVPAGREKLAARFPAFEHATMRGGWSGIYMMSPDSRPIIDQVPQVPGLFVM
                     LGDSGTSFKTAPAIGKCLAEWVVNGEPQTVDLTPFRSTRFAEGKPWKDEDAYSPKRQT
                     ISR"
     gene            complement(3028..4116)
                     /locus_tag="VFV93_16205"
     CDS             complement(3028..4116)
                     /locus_tag="VFV93_16205"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX5166950.1"
                     /translation="MYQRLYKHGIARVDAETAHHLALRGLARASTLPGGLSALTRSLP
                     EPDERLRLRVWDLPFANPLGVAAGLDKDGAAVEALIALGFGHVEVGTITLRPQPGNDR
                     PRIWRVPEEHAVINAMGFPSSGAAAVRAHLIGLRAKGVVGINIGKNRDTPLEAAAEDY
                     AGLVATLFPIANYFTINISSPNTPGLRSLQMADELAHLLATVRDANQHAADLARRDPK
                     PLLVKIAPDLTDAEIEGVAEAAVANGARGIVATNTTTSRSGLPPTYADLPGGLSGPPL
                     REQADHVVRVLYRRVGNQVPIIGVGGISSGADAVARIRSGATLVQLYTAFTWAGPSLP
                     AQILRDLSADADRTGWRSILDIVGIDGG"
     gene            complement(4200..4784)
                     /locus_tag="VFV93_16210"
     CDS             complement(4200..4784)
                     /locus_tag="VFV93_16210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5166951.1"
                     /translation="MPRDFGAWLDPFAGLLVADRAALDIGCGNGHDTRVLLDAGLTVA
                     AFDLEPGNIQRASRRAPAASFVVADLRTGLPFLPCTFDLIVASLSLHYFDRATTNRII
                     CDIQRVTRPGATLLARVNAVGDTASLWGVGTEHEPDVFEVRPGHIKRFFTEESLSEAL
                     EPCFSVLTIDRQETIVTGQRPKQTLVVSATRHDP"
     gene            4867..6243
                     /locus_tag="VFV93_16215"
     CDS             4867..6243
                     /locus_tag="VFV93_16215"
                     /inference="COORDINATES: protein motif:HMM:NF005034.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5166952.1"
                     /translation="MSEDLAGRIDQHIDENLAVYIEDLRALCQLPSVAAQGQAIDDTA
                     DLTRTLLEKYGISAKIMPTARFPVVYGEASGASGQTVLCYNHYDVQPAEPLELWDSEP
                     FDVVEQDGKLYGRGVGDDKGHIVCRLAAIDALRAVTGGLPCGVKFLIEGEEEIGSGSL
                     DAFIAANAGMLKADGCLWEFGGVDYDGKAQIFAGMRGDLYVELRAKTATKDAHSGLGG
                     SQFPNAAWRLTWALATLKGQDERILIPGWYDDVREASARDMELLEALPDDEDLIRKTY
                     GLDSFLLGVTGLERKRRSLFEPTCTISGLSSGYQGAGSKTVLPAEAMAKVDFRLVPNQ
                     RATDLLDKLKRHLAEQGFGDIEVIQHGGYDAARVDPDAPFVRMVADTAWDVYDRPAIV
                     SPTSGGSGPMASFVQHLGMPIANVGIGYPDSGPHAPNEHIRIEDFVRGIKQTARVFLR
                     MADLEVQG"
     gene            6240..7058
                     /locus_tag="VFV93_16220"
     CDS             6240..7058
                     /locus_tag="VFV93_16220"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5166953.1"
                     /translation="MSDQPRSNRRWLDRVRGLRDRATREREGVCYVEGIRQVLSAQEG
                     GHRLEAVLVDPVRLKSEVAWQAVTAARAAGAEYVALTTREFERISSRDNPVGIAAIVS
                     WAVGDLDSLTAVPDGLYLIADDVRDPGNLGTLIRTLDAAGGQAVVVLAGTDPSHPNAL
                     RASLGTTFRVPVYRAASHEELFVWTAQHGVTTIATSAKSDHGLWQMPVRFPAAVLVGN
                     EGDGLAAETVARCDLPVAIPMLGTATSLNVSVAAGVVLYEVRRKALGSSAISSS"
BASE COUNT         1235 a         2044 c         2704 g         1118 t
ORIGIN      
        1 cgaacatccg ttgcttcatc gttcagtagt gaccgatgct gccgtcgcgg cgaacgcggt
       61 cggtagccca gcggagcgag agccaggcga gcgggaccag caacgccgtc gagatcgcca
      121 gcgccaacag gctgccggcg atgtcgcgcg ggccggcgcc gaccagtgtg gcacggcgca
      181 ggccgtccag cgcccacgag agcgggacga ggtaggagat cgggcgggcc caggccggaa
      241 ggagcgagat gggaaagagg acgccgccga agacggtcgt gacgatgttg ccgaggatcg
      301 cgatcgagtc gccgcgcttg acgacgagga tggcggaggc ggcgatcagg ccgagtgagg
      361 cgaacgacgc caggctgagg acgatgaccg gcagggccgc ccagacgttg atatcaaact
      421 tgacacccat cgcgatgacg ccgacggtga cgaagacggc cagccggacg gtggcgagca
      481 gatagtccca ggcggtcgag agggccagca gggcagacgg cggcgtcgcc gtcacgaaca
      541 tcgcttcaag ggtaccggtt gtctgggctt gtcgcaggcg gttggaagcc gagatgagcg
      601 agatcgagaa gtagccggag acggccaggc caatcagggc aaacgggaag tagtcaccgc
      661 cgtatcgttg cagcggttcg gattcgccgc cgacgagaag accgattgcg tagaagagga
      721 tcagcgatga gccgacgccg agcgtctcga tcagtagcat gctgcgatag ctggtctcgt
      781 tgacccagtc tcgccgcagg aaggcgcgga ggagcgccag cgttctcatc ggacggccct
      841 gaactgcccg gccggctcga ccgagatgat ctcgccgccg ccggagacaa tcgccgcgac
      901 ggcagcggcg agggcgtgac cgtcgccgag gtcatcgatc gccagcgtat cggtatcatc
      961 ggacatcatg agagcttgtc gctgctcggt ggtcaggccg cgaacctgga gcaggtagcg
     1021 ttgtggttca cgctcgtcgg aggcggtcgt gatctgaccg tggtcgaggc ggatgacgcg
     1081 gttggcgagg ctgacggcca ggccggcgtc gtgggacgcg atcatggcgg ttgtctcgcc
     1141 ggtggcgaga actgaccgga gcaggctcat gacgtggccg cgatgctcga gatcgaggtt
     1201 ggcggtcggt tcgtcgagca gaagcacgcg cgggtggtgc agtagggcac gggcgaggcc
     1261 gagccgctgg cgcatgccgg tcgagtagcc ggagaagcgc cggtcggcgg cgaacgacag
     1321 gctgagcgtg tcgaggagct cgttcacacg ctggcgggca atggtggtcg agagaccctg
     1381 caactctgcg aagaagagca gattttcccg gccggtgaga cgccagaaga acgagcggtc
     1441 gtcgccggtc atgacaccgg ccagccggcg cacacctttc ggctggctgg cgacatcgta
     1501 gccacagaca cgggcggttc ccgacgtcgg caggatgacg gtagccagaa tgcgcagcag
     1561 cgtggacttg cctgccccgt tgggtccgac gagggcgacg cactcgccgg gctcgacgct
     1621 gaacgacatg ccatcgaggg cgcggttacc ctccagcgag gcacgccgga agagccggcc
     1681 cggctcccgg aaatcgcgcg tgagttgatt ggtctcgatc gccggagtca tcggcggccc
     1741 ttcgacggcg ggcgggacgg cgtcagaatt gtagcgggtg gttgccgggc ggcgtgcgat
     1801 actcgcgcca gtcaatcgct acctgggaag gcaggagttg aggatggcgg attacacagc
     1861 cgacgcggtc gtcatcggcg gcggcgtcaa tgggacgagc gcggcctttc agctggcaaa
     1921 gatggggatg acgaacgtta ttctggtcga gcggcgacag cttggcgccg gggcgaccgg
     1981 gaagtccggc gcgctggtgc ggatgcacta caccaacacg ccggaatcgc agctggcgtt
     2041 cgagagcttc aagatattca aagcgtttca ggacgaggtt ggcggcgatc cgggatggga
     2101 ggggccgggc ttcgtccaga tcgtcgctcc ggggtacgag gaggcactgg cccgcaacgt
     2161 cgaacgacag cagggtctgg gcatcgacac gcggatggtg tcgaaggagg agttgaagga
     2221 aatccagccg ggcatctacg tcgatgacat cggcgcggcc gcctacgagc cgcagtcggg
     2281 ctttgccgac cccaacgcga cggcatattc gttcgcgcag gcggccgaga agctgggcgt
     2341 caagatctgg acacagtgcg aagcgaccgc gatcgtgcgc gacggggaca aggtcgtcgg
     2401 ggttgagacg gagcggggac gaatcgcgac caacaccgtg attctggcac cgggcgcgtt
     2461 tgccgaccgg ctgctcaagc cgttggggct cgactacgga ctctacccgc atcgctcgat
     2521 ggtggcgatc ttccactggc cggttggctg gtcggaccgg catgtggttg tgatcgacaa
     2581 catccaccac acctggctgc ggccggaagg cacacggggg acgctgatcg gtctggaaca
     2641 gccccggcag gagtgggacc ccgagaactt cgaggaaggg gtcgaaggaa gcttcgtgcc
     2701 ggccggccgc gagaagctgg cggcgcgttt cccggccttc gagcacgcga cgatgcgcgg
     2761 tggctggtcg ggcatctaca tgatgagtcc ggactcgcgg ccgatcatcg atcaggtgcc
     2821 gcaggtgccg gggctgttcg tgatgctggg cgattccggc acctcgttca agacggcgcc
     2881 ggcgatcggc aagtgcctgg ccgagtgggt cgtcaacggt gagccgcaga cggtcgacct
     2941 gacgccgttc cgctcgaccc gcttcgccga gggcaagccg tggaaggacg aggacgccta
     3001 cagtccgaag cggcagacga tctcgcgcta acccccgtcg atgccgacga tgtccaggat
     3061 ggagcgccag ccggtgcggt cggcgtcggc gctgaggtcg cgcaggatct gggcgggaag
     3121 cgacgggccg gcccaggtga aggctgtgta gagctggacg agggttgcgc cggagcggat
     3181 gcgggcgaca gcgtccgcgc cgctgctgat gccgccgacg ccgatgatgg gaacctggtt
     3241 tcctacgcga cggtaaagga cgcgaacgac gtgatcagcc tgctcgcgaa ggggcgggcc
     3301 gctcagaccg ccaggcaggt ccgcgtaagt gggcgggagg ccgctgcggc tggtggtggt
     3361 gttagtggcg acgatgccgc gtgcgccgtt ggcgacggcc gcctcggcga cgccttcgat
     3421 ctcggcgtcg gtgaggtcag gcgcgatctt gaccagcagc ggcttcggat cgcgacgggc
     3481 gagatcggcg gcgtgctggt tggcgtcgcg aacggtggcg agcaggtgag ccagctcgtc
     3541 ggccatctgg agcgagcgca ggccgggcgt gttcggcgac gagatgttga tcgtgaagta
     3601 gttggcgatc gggaagaggg tcgcgaccag cccggcgtag tcttcggcgg cggcttccaa
     3661 cggtgtgtcg cgattcttgc cgatgttgat gccgacgaca cctttcgcac gcaaaccgat
     3721 caggtgggcg cgaacggcag cggcaccgga actcggaaag cccatcgcgt tgatgaccgc
     3781 atgttcctcg ggaacgcgcc agatgcgggg ccgatcgttg ccgggctgag gccggagcgt
     3841 gatcgtgccg acctcgacat ggccgaagcc gagtgcgatc agggcttcga cggcagcgcc
     3901 gtccttgtcg agtccggcgg cgacgccgag cgggttggcg aatggcagat cccagacacg
     3961 caggcgaagc cgttcgtccg gttcgggcag cgagcgcgtc agggcgctga ggccgccggg
     4021 gagcgtcgaa gcgcgggcga ggccccgcag tgcgagatga tgcgccgttt cggcgtcgac
     4081 gcgagcaatg ccgtgtttgt agagccgctg gtacatcgga cctcacgttg ttcgatcgtc
     4141 agcgatgagg ccgcggccgc ggccgtcgcc cctatgcgga tatcgtgcca cgtttgatgt
     4201 tatggatcat gccgtgttgc gctgacgacc agcgtctgct tgggccgctg accagtgacg
     4261 atcgtttcct gccggtcgat tgtcaggacg gaaaagcagg gttccagggc ttcggagagc
     4321 gattcttcgg tgaagaagcg cttaatgtga ccgggtctga cctcgaagac atcgggttcg
     4381 tgctcggtac cgacgcccca gagggaggct gtatcgccga cggcgttgac gcgggcgagg
     4441 agcgtcgcac cgggtctcgt gacgcgctgt atgtcgcaga tgatccggtt ggtcgtggcg
     4501 cggtcgaagt aatggagcga gaggctggcg acgatcaggt cgaaggtaca gggcaggaat
     4561 ggcaggccag tccgcaggtc ggcgacgacg aacgatgctg cgggcgcgcg gcgcgaggcg
     4621 cgctggatgt taccgggctc aagatcgaac gcggcgaccg tgaggccggc atcgaggagc
     4681 acccgcgtgt cgtggccatt accgcagccg atgtctagag ctgcccgatc cgcgaccagc
     4741 agaccggcga agggatcgag ccaggcgccg aagtcgcgcg gcataggtgc tccttccggt
     4801 ggcggagatg tcgtggggtc atgttagcat ctggcgcgac gggtcatcag acgagggagg
     4861 gacttgatga gtgaggatct ggccggacga atcgaccagc acattgacga gaatctggcg
     4921 gtgtacatcg aggacttgcg ggcgctgtgc cagttgccga gcgtcgcggc ccaggggcag
     4981 gcgatcgatg acactgccga cctgacacgg acactgctgg agaaatacgg catctcggcg
     5041 aagatcatgc cgacggcgcg ctttccggtc gtttacggcg aagcgagcgg ggcgtcgggg
     5101 cagacggtgc tctgctacaa ccactacgat gtccagcccg ccgaaccgct ggagctgtgg
     5161 gactcggagc cgttcgatgt cgtcgagcag gacggcaagc tctacgggcg cggcgtcggc
     5221 gacgacaagg ggcatatcgt ctgccggctg gcggcgattg acgcgctccg ggcggtgacc
     5281 ggcgggttac cctgcggcgt caagttcctg atcgagggcg aagaagagat cggttccggc
     5341 agcctggatg cattcatcgc ggccaacgcg gggatgctga aggcggacgg ctgcctctgg
     5401 gagttcggcg gcgtcgacta cgacggaaag gcacagatct tcgccggcat gcgcggcgac
     5461 ctgtacgtcg agctgcgggc gaagacggcg acgaaggacg cgcactccgg gctgggtggc
     5521 tcgcagttcc ccaacgcggc ctggaggctg acgtgggcgc tggcgacgct gaagggtcag
     5581 gacgagcgga ttctgattcc cggctggtat gacgatgtgc gtgaggccag cgcgcgggat
     5641 atggagttgc tggaggcgct gccggatgac gaggatctga tccgcaagac gtacgggctg
     5701 gacagcttcc tgctgggcgt gaccgggctg gagcgcaagc gtcgctcgct gttcgagccg
     5761 acctgcacga tttcggggct gtcaagcggc taccaggggg ccggctcgaa gacggtcctg
     5821 ccggccgagg cgatggcgaa ggtcgatttc aggctggtgc cgaaccaacg tgcgaccgat
     5881 ctgctggaca agctgaagcg gcatctggcc gagcaggggt tcggcgacat cgaggtgatc
     5941 cagcacggcg gctacgacgc tgcgcgggtc gatccggatg cgccgttcgt gcggatggtg
     6001 gccgacacgg cctgggatgt ctacgaccgg ccggcgatcg tctcgccaac cagcggcgga
     6061 tcgggaccga tggcgtcatt cgtccagcat ctggggatgc cgatcgcgaa cgttgggatc
     6121 ggctacccgg actccgggcc gcacgcgccg aacgagcaca tccgcattga ggacttcgtg
     6181 cgcgggatca agcagacggc gcgggtcttc ctgcggatgg ccgatctcga ggttcagggg
     6241 tgagcgacca gccccgctcg aaccgacgtt ggctcgaccg cgtgcggggt ctgcgcgacc
     6301 gggcgacccg cgagcgcgag ggtgtctgct atgtcgaggg catccggcag gtgctctcgg
     6361 cccaggaggg cggccaccgg ctggaggccg tcctcgtcga tccggtgcgg ttgaagagcg
     6421 aggtcgcctg gcaggcggta acggcagcgc gagcggccgg cgcggagtat gtggcgctga
     6481 cgacgcgtga attcgagcgg atctcgtcgc gcgacaaccc ggtcgggatt gcggcgatcg
     6541 tcagctgggc cgtcggagat cttgactcgt taacggcggt cccagatggg ctgtacctga
     6601 tcgctgacga cgtgcgtgat ccggggaact tgggaacctt gatccggacg ctggacgcgg
     6661 ccggcgggca ggcggtcgtt gtgctggccg ggacggaccc gagccatccg aacgcgttgc
     6721 gggcgagcct cgggacgacg ttccgggtgc ccgtctaccg ggcagcgtcg cacgaggagt
     6781 tgttcgtctg gactgcccag catggcgtga cgacgatcgc gacctcggcc aagtcggacc
     6841 acgggctgtg gcagatgcca gtgcggtttc cggcggcagt actggtcggg aacgagggtg
     6901 acgggttggc ggccgagaca gtcgcccgtt gcgacctgcc agtggcgatc ccgatgcttg
     6961 ggacggcgac ctcgctgaac gtgagcgtcg cggcgggggt ggtgctgtac gaagtgcggc
     7021 gaaaagcgct tggttcgtct gctatctcat ccagttgacg gaggacctcg tggctgcagg
     7081 aaccatcgtc atcatcaacg g
//
LOCUS       DASUGJ010000715         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_535996, whole genome shotgun sequence.
ACCESSION   DASUGJ010000715 DASUGJ010000000
VERSION     DASUGJ010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535996"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1010)
                     /locus_tag="VFV93_16225"
     CDS             complement(192..1010)
                     /locus_tag="VFV93_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166954.1"
                     /translation="MTRTKTYILTAVSALLFLFLSWAFADGLFALDNLFSGHYYSEYT
                     IISYLMLAAIIAAGIFQARRLPEGGVETPVVRETSTPGQTDDPTIWKLLMGNAYWAVI
                     WMPIRFFVGLEWLQAGEHKLRDDAWMSGGSALQGFWERAVAVPEPPGRAAITYDWYRD
                     FLQYMLDNEWYTWFAKVVAVGEFMIGLGLIVGALVGIAAFFGTLLNFSFMLAGTTSSN
                     PVLFGLTVFLVLAWKVAGYVGLDRWLLPALGAPWSFGTLFKGGAPVRHNDRATA"
     gene            complement(1291..2913)
                     /locus_tag="VFV93_16230"
     CDS             complement(1291..2913)
                     /locus_tag="VFV93_16230"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5166955.1"
                     /translation="MKTESPGEAQLPDHIAGLLKPDAYPHPVEHVELIQTHISYVLMA
                     GDMVYKLKKPLDLGFLNFTTLRRRHYYCRQEVELNRRLCGDTYLGVVPVTRQGRRYRL
                     DGRGEVIDYAVQMRRLPSDRMMDRVLADGRLTPEMLERLADKIARFHASSATGPQVER
                     YGSPRTIERNWRENIDQTRPFVGRTLSSWQADFIEAAALAFLTRHRHAFEERVRAGRI
                     RDCHGDLRASAVCFTDDICVYDCIEFNRRFRYADVASEVAFLAMDLDRRGRPDLADRF
                     VARYQQTTGDDDLLRVLNFYKCYRAYVRGKVNSFQLDEPEIAEAAKATARTSARHYFE
                     LACRYAASDRLPIFIIMTGLSGTGKSAVASALAAKRDLAIISSDIVRKELAGIAEVDH
                     QYEAYERGVYSREFTQQTYAALLDRARQLLAEGRSVIVDATFQRREWREQAFDLARQA
                     GALVFCLETVADESIVRQRLVAREHDQAAISDARWETYLSQASRFEPVMEVGDWQHIC
                     LDTGQPFGDVVASAMNALEARLHPVALSGDRF"
BASE COUNT          593 a          948 c         1026 g          587 t
ORIGIN      
        1 gttctgcgtc tcatcgttga gacaatgatc ggctccggca gggaaatcgg acacgaccgg
       61 cagggtggtt ggctgtgggc agggcggggt agtttccagc ctacccgttg gggtagggcg
      121 gcgggaacag gcgagacccg gtctgcgaga gaccgggcct cgtgggtgct atagcgctag
      181 ctgtctggcc gtcaggcggt cgcgcggtcg ttgtgccgga cgggtgcgcc acccttgaag
      241 agggttccga aggaccacgg ggcgccgagg gccggcagca gccagcggtc gagtccgacg
      301 tagcccgcca ccttccaggc caggaccagg aagaccgtca gtccgaacag aaccgggttg
      361 ctgctcgtcg tcccggccag catgaagctg aagttcagca gcgtcccgaa gaaggccgcg
      421 atcccaacca gggcgccgac gatcaggccc agaccgatca tgaactcgcc gaccgcaacc
      481 accttggcga accatgtgta ccactcgttg tcgagcatgt actgcaggaa gtcgcggtac
      541 cagtcgtagg tgatggcggc ccggcccggt ggctccggaa cggccacagc gcgctcccag
      601 aagccctgca aggcagatcc gccactcatc caggcgtcgt cacgcagctt gtgctcgccg
      661 gcctggagcc attcgagacc gacgaagaag cggatcggca tccagatgac cgcccagtag
      721 gcgttcccca tcagcagctt ccagatggtg gggtcatcgg tctgccccgg tgtggatgtc
      781 tcgcggacga ccggcgtttc cacgccaccc tccggcaagc gtcgtgcctg gaagatcccg
      841 gcagcgatga tcgcggccag catcaggtag gagatgatgg tgtactccga gtagtagtgc
      901 cccgagaaca ggttgtccag tgcgaacagg ccatcggcaa aggcccagct caggaacagg
      961 aacagcagcg ctgagactgc tgtgaggatg tacgtcttcg tgcgtgtcat ttcggtctcc
     1021 aatcttggtc tgagatgtca tcaatgcgcg atcaatggat gtgcttcgtc agttcccctc
     1081 gtcaatctca ccctccgttc gttattgact cgctgatcca tgagcgtcca gttcgttgct
     1141 ggcatcaaca atagacggcg gatgtatgca ttcctgtgaa tgaaatgagc gagacgttac
     1201 gattccgtgt caggaggctg aggagccgca agctgggaca gcttcaggcg atcgctcatg
     1261 gatcggcgcg atgagtgagc atgaccgcag ttagaagcga tcgccactca gtgcgacggg
     1321 atgaagcctc gcttcaagtg cgttcatcgc gctggcgacg acgtcgccga agggctgccc
     1381 agtatccagg caaatgtgct gccaatcgcc gacctccatc actggttcga accgcgaggc
     1441 ctggctgaga tacgtctccc agcgcgcatc agatatggcc gcctggtcgt gttcgcgagc
     1501 gacgagccgc tgccgcacaa tgctctcatc ggcgacggtt tcgaggcaaa agacgagcgc
     1561 gccagcttgc cgcgccaaat cgaaggcctg ctcgcgccac tctcgccgtt ggaatgtggc
     1621 atcaacaatg actgatcgtc cctcggcaag caactggcgt gctcggtcga ggagggctgc
     1681 atacgtctgt tgcgtaaact ccctggaata gacgccgcgc tcgtacgcct cgtactgatg
     1741 gtctacctcg gcgatgcctg caagttcctt tcggacaatg tcggacgaga tgatcgctag
     1801 atcgcgtttg gccgccaagg cgcttgcgac ggctgacttc cccgttccgg acagtccggt
     1861 catgatgatg aagatcggca gtcggtcgct ggccgcatag cggcaggcga gttcaaaata
     1921 gtggcgtgcg gatgttcgtg ccgtggcttt cgccgcctcg gcgatctcgg gttcgtcaag
     1981 ttggaagctg ttgaccttgc cgcgcacata cgcccggtag cacttgtaga agttgaggac
     2041 gcgcagcaga tcgtcgtccc ctgtagtttg ctggtaccgc gcgacgaatc ggtcggccag
     2101 atccggtcga ccacgccggt cgagatccat ggccagaaac gcaacctccg acgcgacatc
     2161 ggcgtagcgg aagcggcgat taaactcgat gcagtcgtag acgcagatgt cgtcggtgaa
     2221 gcagacggct gacgcgcgca gatcgccatg gcagtcacgg atacgtccag cgcgaacccg
     2281 ctcttcgaag gcgtggcgat ggcgcgtcag aaaggcgagg gcagctgcct cgatgaagtc
     2341 ggcctgccag gaggacagcg tgcggccaac gaatgggcgc gtctggtcga tgttctcgcg
     2401 ccagttgcgc tcgatcgtgc gcggggagcc gtagcgctcg acctgtgggc cggtcgccga
     2461 cgaggcgtgg aagcgggcaa tcttgtccgc cagccgttca agcatctcag gcgtcagtcg
     2521 tccgtcggcg aggactcgat ccatcatgcg gtcgctgggc agccggcgca tctggaccgc
     2581 gtagtcgatg acctcacctc gaccgtcgag ccgatatcgc cttccctgcc gcgtcaccgg
     2641 cacgacgccg agataggtgt cgccgcacag gcgccggttg agttccacct cctgacggca
     2701 gtagtagtgt cgccgccgga gcgtcgtgaa gttgaggaag ccgaggtcga gcggcttctt
     2761 aagcttgtag accatgtcgc cggccatcag cacgtaggag atgtgcgtct ggatcaactc
     2821 gacatgctcg accggatgcg ggtacgcgtc cggcttcagc aagcctgcga tgtgatcagg
     2881 tagttgtgct tcacctggcg actcggtttt cacctggcgc tcctccgcaa tcgactgggg
     2941 cgcggaccga ggctccgcgt ccttcgtcct cgagatggcc cgaacccgcg tcaggttaca
     3001 gccgatgcaa cccacgcgcg aatgcggcca gttcatcgcg gaaggcggga tgagcgatgg
     3061 cgatcagggc ttcggcgcgc tggcggaggc tcttgccgta cagatctgca ataccgtatt
     3121 cggtcacgac gtaatgcagg ttgccctgga caag
//
LOCUS       DASUGJ010000716         4062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1923110, whole genome shotgun sequence.
ACCESSION   DASUGJ010000716 DASUGJ010000000
VERSION     DASUGJ010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923110"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VFV93_16235"
     CDS             <1..1021
                     /locus_tag="VFV93_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166956.1"
                     /translation="LAVVKAEPLNGPDEKDAASYIYLKKALIHLFNSETDAAQEPQAM
                     FIQLQTEVADYYRERSGHNQTLCADMREVERGDTLTASGSYSQATDAYRQALSLNADN
                     ALASDGLSFALYRQSDTTGAIEAARQSTEIYPDNPRPWARLGLYALVNNDTSTRDQAY
                     GRFLSLIAERPPQERLALAKEAITDLQDLVRDQPQLSATALELLPQLREFADGISDAG
                     DAYQYPQLYSELGKFALLAGDTNAAQTLLQRGIALDAHQPIAKVWLAMAALIDGDDAG
                     NEIQAVVDELNDPLWAETEGVEAFGRDDLTELAQTEIEEFVAARPDDGNTFDSLVQQL
                     NRSVD"
     gene            1089..1394
                     /locus_tag="VFV93_16240"
     CDS             1089..1394
                     /locus_tag="VFV93_16240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5166957.1"
                     /translation="MAWQIQRADGELNDVVARSIEDGPQTIVREGTEVAVVMSIGDFR
                     KLTSEHEATANLECAGSKQDLRDFLLSRDGPFFDDLAIELERIEQERIDNLFRDVEL"
     gene            1685..3967
                     /locus_tag="VFV93_16245"
     CDS             1685..3967
                     /locus_tag="VFV93_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5166958.1"
                     /translation="MSATVEEGTIIGQPMKRIEGDKKVTGATRYVDDLQLPGMLHGRL
                     VTSIYAHAEINGIDASAALAVPGVVAVYTGKDVLPAGSEPADRNHHMLARDKALYYGQ
                     PVAVVVATSESIAVEAAGLVEVDYTPLEAVVDPLRAMERDAPVVRKKATDGSEWAEGQ
                     MHATVSGGDDLDISKLPDNITNAASFSRGDVAAGFKAADQIVERRYTTNWVHQSYIEP
                     HGSVVVPDPLGNLTIYTTTQGQFYTRNATAEILGLPQNQVKVVGMEVGGGFGGKAVML
                     EPLAGWIALRHKLPVKINMTRSEELSAGNPAPGSVIDIKIGGKQDGTITAIQARLVFD
                     SGCYPGTPVTVAALMSGGYYRTDNLELVGYEVLTNKPGNGAYRAPGAPQATFAIEQAV
                     DELAGKVGWDPVEFRLHNCSVEGDPQPNGVPWPRIGLKEILEVVRDHPAYKNRAAGPH
                     KGVGVAIGGWPGGVEPCAANIRVNTDGSLVVQLGSTDITGTNTVMSQLAAEAFGTTPD
                     NVTIESADTGAAPYAGMSGGSKVTYTVGPAVIRAAQAAREQVLAIAAAEMEVSPDDLE
                     LARGKVTVKGAPDKEMSIAAIAGKSMTFGGKYEPVYGIGKSAQTDRAPGFSGQIADVS
                     IDPDTGAVTIDRYVTVQDVGKALNPAQVEGQMIGGTVQSIGFAMYEAIQYGEDGQLLS
                     STLMDYVIPKSFQVPPIETVIVEVPARSGPYGAKGIGEPPIIPGPAALANAIAAATGS
                     RVLEAPFTPERVRKAVSNGA"
BASE COUNT          805 a         1282 c         1330 g          645 t
ORIGIN      
        1 actggcggtt gtcaaagccg agccactgaa tggcccggac gagaaggacg cggcctccta
       61 catctacctc aagaaggcgt tgatccatct cttcaacagc gagaccgatg cggcccagga
      121 gccgcaggcg atgttcatcc agctccagac agaggtggct gactactacc gcgagcgctc
      181 aggccacaac cagacccttt gcgccgacat gcgcgaggtc gagcgcggcg atacgctgac
      241 agcatccggc agctacagcc aggcaaccga cgcctaccgg caggcgttga gcctgaatgc
      301 cgacaatgcg ctggcgtcgg atggactctc gttcgcactc taccgacaga gtgacactac
      361 cggcgcgatc gaggccgccc gtcagtcgac cgagatctat ccagacaatc cccgcccgtg
      421 ggcacggctg ggactctatg cgctggtcaa caacgacacg tcgacccgcg accaggccta
      481 tgggcgattt ctctcgttga tcgccgaacg tccgccgcaa gagcggctgg cgctggcgaa
      541 ggaggcgatc accgatctac aggacctggt gcgcgaccag ccgcaactga gcgcaaccgc
      601 gctggaactg ctgccgcaac tgcgggaatt tgccgacgga atctcggacg ccggcgacgc
      661 gtaccagtat ccgcagctgt acagcgaact cggcaaattc gcgctgctgg ccggcgacac
      721 gaacgctgca cagacactcc tgcagcgtgg gatcgcactg gatgcccacc agccgattgc
      781 aaaggtctgg ctggcgatgg cggcgttgat cgacggtgat gacgcgggca acgagataca
      841 ggcagtggtt gacgaactga atgacccact ttgggcggag acggaaggcg ttgaggcgtt
      901 cggacgggat gatctgacgg aactggctca gacggagatt gaggagttcg tggcagcgcg
      961 tccagacgac ggcaacacgt tcgactccct cgttcagcag ttgaatcgct cggtggacta
     1021 ggctcaacac atgttgttgc ccatgacgcc cgttgctatg atcgatgcag actggacagg
     1081 aggctgctgt ggcatggcag atccagcgcg cggatggaga actcaacgac gttgttgctc
     1141 gttcgataga ggatgggccg caaaccatcg ttcgtgaagg aaccgaggtc gcggtggtca
     1201 tgtcgatcgg cgatttccgg aaactcacga gcgagcatga agcaaccgct aacctggaat
     1261 gtgccggcag caagcaggac ctgcgtgact tcttactttc acgtgatggg ccattcttcg
     1321 atgacctcgc catcgaactg gaacgcatcg agcaagaacg tatcgataac ctgtttcgag
     1381 atgtcgaact gtgaagttcc tgctcgaact gtgatgttcc tgctcgatat gaacgtcatc
     1441 tctgagacac gcacgcgacg acccggtcca atgcaccgcc cggctcgacc ggcacggtat
     1501 tgcgatcctg aacgccttcg agccgagtcg ctgacaaacc acacttgacg tgcaagctga
     1561 ttccggcgaa gatgccggtc tgtcaccgtg cgttccgggg ccggttctgc cgccagatgt
     1621 tgtcaacggt cgatgtgtaa cgcgcggggt ttcgcaggcc atccataggg actaagggag
     1681 gaccatgtca gccacggttg aagaagggac aatcatcggc cagccgatga agcggatcga
     1741 gggggacaag aaggtaaccg gcgcgacacg ctacgtcgat gacctgcagt tgccggggat
     1801 gctgcatggg cggctggtga cgagcatcta cgcccacgcc gagatcaacg ggatcgacgc
     1861 cagcgcagcg ctggccgtgc cgggggttgt ggccgtctac accggcaagg atgttctacc
     1921 agcgggcagc gagccggccg accgcaacca ccacatgctg gcgcgcgaca aggcgctcta
     1981 ctacgggcag ccggtcgccg tcgtcgtcgc gaccagcgag tcgatcgcgg tcgaggcggc
     2041 tgggctggtg gaagtcgatt acacgccgct ggaggccgtc gtcgatccgc tgcgggcgat
     2101 ggagcgggat gcgccggtcg tgcgcaagaa ggcgaccgac ggctctgagt gggccgaagg
     2161 gcagatgcac gcgaccgtct ccggcggcga cgatctggat atctcgaagc tgccggacaa
     2221 catcaccaat gcggccagct tctcacgcgg cgatgtcgcg gccgggttca aggctgccga
     2281 ccagatcgtc gagcgacgct acaccaccaa ctgggtccac cagtcgtaca tcgagccaca
     2341 cggctcggtc gttgtccccg acccgctcgg caacctgacg atctacacaa ccacccaggg
     2401 ccagttctac acacgtaacg cgacggctga gattctggga ttgccgcaga atcaggtgaa
     2461 ggtagtcggg atggaggtcg gcggcggctt cggcggcaag gccgtcatgc ttgagccgct
     2521 ggccggctgg atcgcgctgc gccacaagct gccggtcaag atcaacatga cccgctcgga
     2581 agagctctcg gccggtaatc ccgcccccgg ctcggtgatc gacatcaaga tcggcggcaa
     2641 gcaggacggc acgatcacgg ccatccaggc gcgactggtg ttcgactccg gctgctaccc
     2701 gggtacgccg gtgacagtcg cggcgttgat gtcgggcggc tactaccgga ccgacaacct
     2761 ggagctggtc ggctacgagg tgctgaccaa caagcccggt aacggcgcct accgtgcgcc
     2821 gggcgccccg caggcgacct tcgcgatcga gcaggccgtc gatgaactgg ccggcaaggt
     2881 cggctgggat ccggtcgagt tccggctgca caactgctcg gtcgaaggcg atccacagcc
     2941 gaacggcgtt ccctggccgc ggatcggact gaaggagatt cttgaagtcg tccgcgacca
     3001 tccggcatac aagaaccggg ccgccggccc gcacaagggc gtcggcgtcg cgatcggcgg
     3061 ctggccgggc ggcgtcgagc cgtgtgcggc caacatccgc gtcaacaccg acggctcgct
     3121 tgttgtccag ctcggctcga ccgacatcac cggcaccaac acggtcatgt cccaactggc
     3181 cgccgaggcg ttcgggacga cccccgacaa cgtgacgatc gaatcggccg acaccggcgc
     3241 tgccccgtac gcggggatga gcggcggctc caaggtcacc tacacggtcg gcccggccgt
     3301 catccgcgcc gcccaggcag cacgcgagca ggtgctggcg atcgcggcgg ccgagatgga
     3361 agtctcgccg gacgacctcg agctggcgcg cggcaaggtg acggtcaagg gcgcgcccga
     3421 caaagagatg tcgatcgcgg ccatcgccgg caagagcatg acgttcggcg gcaagtatga
     3481 gccggtctac ggcatcggca agtcggccca gacagaccgc gcgccgggct tctccggcca
     3541 gatcgccgac gtttcgatcg acccggatac cggcgctgtg acgatcgacc gctacgtcac
     3601 cgttcaggac gtcggcaagg cgctcaatcc ggcccaggtc gaggggcaga tgatcggcgg
     3661 aacagtccag tcgatcggct ttgcgatgta cgaggcgatc cagtacggcg aggacggcca
     3721 gctgctgtcg tcgacgctga tggactacgt gattccgaag tcgttccagg tgccaccgat
     3781 cgaaacggtg atcgtcgaag tgccagcccg ctccggcccc tacggcgcga agggcatcgg
     3841 cgaacctccg atcatccccg gcccagccgc gctggccaac gccatcgccg ccgcaacggg
     3901 ctcgcgggtg ctggaagccc cgttcacacc ggagcgggta cgcaaggcgg tcagcaacgg
     3961 ggcatagaca cgccttccac taattcgaga cgaaccacgg cgggccgatc ggcccgccgt
     4021 ggttcgtctc gaattagtgg aaggcgtgtc tatgccccgt tg
//
LOCUS       DASUGJ010000717         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1506180, whole genome shotgun sequence.
ACCESSION   DASUGJ010000717 DASUGJ010000000
VERSION     DASUGJ010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506180"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VFV93_16250"
     CDS             complement(<1..720)
                     /locus_tag="VFV93_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5166959.1"
                     /translation="MSAKNRLTRHTAVSGSWLLLAVLLLLVPLLAACGGDDDDATPIP
                     TATKPAGSASPTGAASTSEPAGDGASPTPGASGEPAATTEPGDTLMGVEIEPAAHEGG
                     TFIEGSSADLQTVNPIIANDSPTLNFLALIYEPLVELHPETLEPVGVLADSWEVSSDG
                     LTWTFRLRDGVTWQDGEPFTAKDVKFTYELHMNEASNSSYGGDLLAKIGAIEVVDPLT
                     VAFTLPQPYADFAVDVATYGII"
     gene            1002..2387
                     /locus_tag="VFV93_16255"
     CDS             1002..2387
                     /locus_tag="VFV93_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166960.1"
                     /translation="MLKWLSSVMMAMLLVLALAGCGGGDDDDDPTATATTQATTPAAT
                     ATATSSVSGGAAASPTALTATREPEASPTARATAPSRPIATQPPTATATETTGEGIPS
                     LDAQLMELLLQPEDFTSEWSQDAFGLMESDTDDSDEICGQPPFPDRDQKLAGVEAQYS
                     IGGDQPAFLLHNIVVFPDDTAVDALNYARDISGCGEWTDADGQVYTILPLDGPDRGDD
                     SFMASMSFESNGTPLYGEYTFVRVGGAIATVAFITVDGADTSAHRALVDLAVTRLEDA
                     SIGAGTGALDRMLLTAEDLALVDEVNVWQVGDEIDPSEADRFAVCDSESFPDVLGSAD
                     ESGHEMSANDDAGPYAMHSVVQMLAGDGTTAMDWIRSELSCSSWTDEDGDEYTVSDSG
                     DLEVGDDTFWLIVSLTDEESDTSAQVGFGFSQVGDYISVVGLAAEDTIDPELFGAMMA
                     LAAEKVSVNAP"
BASE COUNT          424 a          782 c          831 g          442 t
ORIGIN      
        1 gatgatgccg taggtggcga cgtcgacggc gaagtccgcg tacggctgcg gcagcgtgaa
       61 ggcgaccgtc agcgggtcaa ccacctcgat cgcgccgatc ttggccagca ggtcgccgcc
      121 gtacgacgag ttgctggcct cgttcatgtg cagctcgtag gtgaacttga cgtccttggc
      181 cgtgaacggt tcgccgtcct gccaggtcac gccatcgcgc aggcggaagg tccacgtcag
      241 gccatcgctg gagacttccc acgaatcggc cagaacacca accggttcga gtgtctcagg
      301 atgcaactcg accagtggct cgtagatcaa cgcgaggaag ttcagcgtcg ggctgtcgtt
      361 ggcgatgatc gggttgactg tttgcaggtc ggcagacgag ccttcgatga aggtgccgcc
      421 ctcgtgagcg gcgggttcga tctcgacgcc catcagggtg tcgcctggct cggtcgttgc
      481 cgcaggctca ccggacgcgc ccggcgtcgg cgaggcgccg tcgcctgccg gctcggatgt
      541 ggaggccgca cccgtcgggc ttgcgctgcc ggccggcttc gtggccgttg gtattggcgt
      601 tgcgtcgtcg tcatcgcccc cacaggcggc cagcagcggc accagcagga gcagcaccgc
      661 cagaagcagc cagcttccgg agacagcggt gtgtcgtgtc agacggttct tggcactcat
      721 cgatgaggtt ccccgattcc aggacgcgtg acgtggcccg cgtcacgcgt acgcatattc
      781 gacctggcaa tcatgacgaa ccctcgggct cctgcacgtc ccccatacgt actggctaac
      841 gcacgaccta cgattgatgg acgcatgcga cgcaaactgg cgtcggtcgc cctggtttgg
      901 aatgcttcgc agccgtactt tcgatcatgg aagaattgac gtgtggtgtg tcggccagac
      961 gctgcgattc gctggtagca gcaatgagga gaatatcgac ggtgttgaag tggctttcga
     1021 gcgtcatgat ggcgatgctg ctggtgctgg cgttggccgg ctgcggcggt ggcgatgacg
     1081 atgatgaccc gaccgcaacc gccacgaccc aggcgacgac gcctgccgca acggcgaccg
     1141 caacctcctc ggtcagcggt ggagctgctg cgtcgccaac ggcactgacg gcgacgcggg
     1201 agccggaggc gtcgcccacg gcgcgcgcta cagcaccctc ccgcccaatc gcgacccagc
     1261 ctccaacggc gacagcgacc gagacgacag gcgagggcat tccgtcgctc gacgcgcagt
     1321 tgatggagtt gctactgcaa cccgaagatt tcacctccga gtggtcgcag gacgccttcg
     1381 ggctgatgga gtcggatact gacgattctg atgagatctg cggccagccg ccgttcccag
     1441 accgcgatca gaagctggct ggcgtcgagg cgcagtactc gatcggtggc gatcagccgg
     1501 ccttcctcct gcacaacatc gtcgtcttcc cggacgatac cgccgttgac gcgctcaact
     1561 atgcgcgcga catctcgggt tgcggggagt ggaccgacgc tgacggtcag gtctacacga
     1621 tcttgccgct cgacggaccg gatcgcggtg acgacagttt catggcgtcg atgtcgttcg
     1681 agtcgaacgg gacgccgctc tacggtgaat acacgttcgt ccgcgtcggc ggcgcgatcg
     1741 cgaccgttgc gttcatcacc gttgacggcg cagacacctc cgcgcaccgg gcgctcgtcg
     1801 acctggcagt cacgcgcctg gaagacgcat caatcggtgc cgggaccggc gcgctggacc
     1861 ggatgctgct gacggccgaa gacctggccc tggtcgatga ggtcaatgtc tggcaggttg
     1921 gcgacgagat tgaccccagc gaagcggacc ggttcgcggt ctgtgattcg gaatccttcc
     1981 cggacgtgct tggctctgca gacgagtccg gtcacgagat gagcgccaac gacgacgccg
     2041 gaccgtacgc gatgcacagc gtcgtccaga tgctcgccgg cgacggcacg accgcgatgg
     2101 actggatcag gagtgaactc tcctgctcct catggaccga tgaggacggc gacgagtaca
     2161 ccgtctccga ttccggcgat ctcgaagtcg gcgacgacac cttctggctg atcgtctcgc
     2221 tgacggacga ggagagcgac acgagcgccc aggtcggctt cggcttcagc caggttggcg
     2281 actacatctc ggtcgttggt ctcgccgctg aggacacgat cgatcccgaa ctgttcggcg
     2341 ccatgatggc gctggcggcc gagaaggtgt cggtgaatgc gccgtaacgc gcgtgccgtt
     2401 attcgcgtag gggcgatggc cgcagcctcg ccctgcccca acgctcggat ggtcagggca
     2461 gggcgaggct gcggccatc
//
LOCUS       DASUGJ010000718         4901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_952889, whole genome shotgun sequence.
ACCESSION   DASUGJ010000718 DASUGJ010000000
VERSION     DASUGJ010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952889"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1013)
                     /locus_tag="VFV93_16260"
     CDS             complement(57..1013)
                     /locus_tag="VFV93_16260"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEX5166961.1"
                     /translation="MRESIERFLSALEAGNDFSTNTVSAYRNDLNQFVGFICDEQGLG
                     SWAELQDSHLTSYVLQMRERKYASSTVARKTAAIKSFFGYMVRSGELRADPSESLVSP
                     RVEKYVPKAMTEQQIQSLLHEPTREAKPENARDCAMLQTLYATGMRVSELVSLDLADV
                     DLERGTVCCTGKQNRRRDVELSPEAVASLTQYLETGRPAIAKVADEPALFLNHRGHRL
                     TRQGFWLILKQYAQAAGIDEITPHTLRHSFAAHQITRGRDLGDVQRILGHVSISTTQI
                     YQKIASDMQEPERLGIVSPCDDEAQEITLEPEAELAGVGVER"
     gene            complement(1093..2205)
                     /gene="ald"
                     /locus_tag="VFV93_16265"
     CDS             complement(1093..2205)
                     /gene="ald"
                     /locus_tag="VFV93_16265"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX5166962.1"
                     /translation="MIVGIPKEIKDNENRVSTTPSGVAEYVSRGHKVLVERSAGVGSG
                     FADSEYEAAGATMIDSHTDVFAQADMIVKVKEPIASEYDLLREDQILYTYLHLAAEEA
                     LTKALIERKVASIAYETVELPNRALPLLTPMSEVAGRMSVQVGAHYLEKVNGGRGLLL
                     GGVAGVPAANVVIVGGGVVGTNAAQIALGMGAHVTILDISIDRLRFLDQVLHGRFQTL
                     ASNRGNIAEAVKHCDLLIGGVLIPGAKAPKLVTAEMVSSMRPGSVIVDVAIDQGGCIE
                     TVRPTSHSNPTFEVDGVVHYCVTNMPGAVPRTSTFALSNVTLPYGLQLAASGMAAVAK
                     DSALAKGVNVYKGHVTYPAVAEAFGLEYTPVDKLLA"
     gene            2554..3882
                     /gene="rseP"
                     /locus_tag="VFV93_16270"
     CDS             2554..3882
                     /gene="rseP"
                     /locus_tag="VFV93_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX5166963.1"
                     /translation="MGFLNALYIIPVLGVLIFLHELGHFVMARRAGVLVEEFGFGLPP
                     RLWGVKRGDTVYSINALPIGGFVRVKGEDGKSFDQDSMQAKTPRQRAGFLAAGSVMNF
                     LTAFVLVVLMVAFQGRTTSNVYIEEVVADSPAAAAGWQPGDRVIAINGQAVDNVDDFS
                     PYTVDFAGQNLNVTILRNGEEIQTTVVPRENPPPDQGRTGIILANYRLASIEITNVPD
                     DSVAAAAGLQEGDQIIAVGDMPATDYMAYAGVIRNHAGQTVDVTVQRDGERVVVPVAV
                     PVNVPANDPPLGEDVVQNLKFERIPIAEVPGESVDLFFGSIKQMGQGLVSLIRGETPL
                     DDLAGPIGMGQLTSEVVSESALPLWVTLANLMFILSLNLGLLNLLPLPALDGGRLLFV
                     LIEVLRGGRRVSPEKEGMVHFVGMVLLLTVMLGIAFLDVERLISGNTFLE"
     gene            3991..>4901
                     /gene="ispG"
                     /locus_tag="VFV93_16275"
                     /gene_synonym="gcpE"
     CDS             3991..>4901
                     /gene="ispG"
                     /locus_tag="VFV93_16275"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX5166964.1"
                     /translation="MSLLAPRRLTKPVYVGDVRIGGGAPIVVQSMATADTRDPKATLR
                     QISELADAGCEIVRIAVPDRKAAAALPDIVPHSPVPLIADIHFEHTLALKALDAGIHG
                     LRLNPGNIRKHEDVREVVKKATERNTPIRIGVNFGSVAPMTQEFVDEMAQQHASQVEL
                     VAEHMARSALGHIQILEDLDYDNIKISLKAFEVPVMLEAYKRMATRNAYPLHLGVTEA
                     GTPRAGSVRSAVGLGTLLALGIGDTIRVSLTTDPVEEVFVAYEILKSLELRQKGATMV
                     ACPTCGRVEVDLFSIANRVEAFLAEIN"
BASE COUNT          874 a         1639 c         1474 g          914 t
ORIGIN      
        1 actctcgtcc cgcccgcccg tcgccaacgt cagatgcggt ctaccatgtt gggtgtctag
       61 cgctcgacgc ccaccccggc cagttccgcc tcaggctcca gcgtgatctc ctgcgcttcg
      121 tcatcgcatg ggctgacgat ccccagccgc tccggctcct gcatgtcgct ggcgatcttc
      181 tggtagatct gcgtcgtcga gatgctgaca tgcccgagga tgcgctgaac gtcacccaga
      241 tcacgcccac gcgtgatctg gtgggcggcg aacgagtgcc gtagcgtatg cggcgtgatc
      301 tcatcgatgc cggcagcctg agcgtattgc ttcaggatca gccaaaaccc ctgccgcgtc
      361 agccggtgtc cgcgatggtt caggaacagg gccggctcgt cggcgacctt ggcaatcgca
      421 ggccggccag tctccagata ctgcgtcagg gacgcgacgg cttccgggct gagctcaaca
      481 tcgcgacgtc ggttctgctt gccggtgcag cagacggttc cccgctccag atcgacgtcg
      541 gccaggtcga ggctaaccag ttcagagacg cgcatgccag tcgcgtagag cgtctggagc
      601 atcgcgcagt cgcgtgcgtt ctccggcttg gcctcacgcg tcggctcgtg cagcagcgac
      661 tggatctgct gttcggtcat tgccttcggg acgtacttct cgacgcgcgg cgagacgagt
      721 gactcagacg gatcggcacg caactcgcca ctacggacca tatagccgaa gaacgacttg
      781 atcgcggccg tcttgcgtgc gacggtcgag ctcgcgtatt ttcgctcgcg catctggagg
      841 acgtacgagg tcagatgcga atcctgcaac tcagcccagg aacccaggcc ctgctcgtcg
      901 caaatgaacc cgacaaactg gttgaggtcg ttgcggtagg cagagacggt gttcgtcgag
      961 aagtcatttc ccgcttccag agcgctcagg aagcgctcga tgctttctcg catgggccag
     1021 ttcctctcgg ttcgagacag ggcaggcgtc cggggccggc gacacattcg agcgccgacc
     1081 ccggcgtgtt tcctatgcca gcaacttatc gacgggcgtg tactcaaggc caaacgcctc
     1141 ggcaaccgcc ggataggtga catggccctt gtagacattg acacccttcg ccagcgcgga
     1201 gtccttcgcg acggcggcca tcccgctggc agccaattgg aggccatacg gcaacgtcac
     1261 gttcgacaat gcgaacgtgc tggttcgcgg cacagcaccc ggcatgtttg tcacgcaata
     1321 gtggacgaca ccgtccacct caaacgtcgg attgctatga ctggtcggtc ggaccgtctc
     1381 gatgcaaccg ccctggtcga tcgcgacatc gacgatcacc gacccgggcc gcatcgaaga
     1441 caccatctcg gccgtcacca gcttgggcgc cttcgcgccg gggatcagga cgccgccgat
     1501 cagcagatcg cagtgcttga cggcctcagc aatgttgccg cgattcgacg ccaacgtctg
     1561 gaaccggcca tgtagcacct gatcgaggaa gcgtaaccgg tcgatcgaga tatccaggat
     1621 cgtcacgtgc gcgcccatcc caagcgctat ctgagcggcg ttcgtcccga cgacgccacc
     1681 gccgacgatg acgacgttgg cggccggtac cccggcgaca ccgccgagca gcaacccacg
     1741 accgccgttg accttctcca ggtagtgcgc gccaacctgg acggacatcc ggccggcaac
     1801 ttcactcatc ggcgtcagca gcggcaaggc acggtttggc agttcgaccg tttcgtaggc
     1861 gattgaggcg accttgcgct cgatcagcgc tttggtcaag gcttcctcgg cggcgaggtg
     1921 aaggtaggtg tagagaatct ggtcttcgcg caggaggtcg tactcggagg cgatcggttc
     1981 cttgaccttc acgatcatgt cggcctgggc gaagacatcc gtgtgggagt cgatcatggt
     2041 cgcgccggct gcctcgtact cgctgtcggc gaacccgctg ccgactccgg ccgagcgttc
     2101 taccagcacc ttgtgcccgc gtgagacgta ttctgcgacg ccggatggag ttgtggacac
     2161 ccgattctcg ttgtccttga tctcctttgg aatcccgacg atcatcctgg atctgttccc
     2221 ttcaccacaa cgccctccgg aggagatcac cggacccaag actccggact gcgcgtctgc
     2281 acgcgcggga aacgcgacat gcgacggcac atgccccatc gggcgcgccc caccacggct
     2341 gtcagactcc ccggcagccg cgtcattcta acatacataa cttcatctcc cgcgcctcga
     2401 caccgcctcc agcatcgccc gaccgacgcc gtaactgctt tcgttgacgc agtcttttca
     2461 gcacaggtac gattcattca ggcgcgaagc catagccggc attctccgcc gccatcgacc
     2521 cggccggcgt catcttcatg agagcagtga acagtgggat tcctcaacgc cctctacatt
     2581 atcccggtcc tcggtgtgct gatcttcctg cacgagctgg gtcacttcgt gatggcccgc
     2641 cgggccggcg tgctggtcga agagttcggt ttcgggctgc cgccccggct ctggggggtc
     2701 aagcgcggcg acacggtcta ctcgatcaac gcattgccga tcggcggctt cgtccgtgtc
     2761 aagggcgaag acggaaagtc gttcgaccag gacagcatgc aggccaagac cccacgccag
     2821 cgcgccggat tcctggcggc cggctctgtc atgaacttcc tgacggcgtt cgtcctcgtc
     2881 gtcctgatgg tcgcctttca gggccggacg acgtcgaacg tctacattga ggaagtcgtc
     2941 gccgactcac cggccgccgc tgccggctgg caacctggag accgcgtcat cgcaatcaac
     3001 ggtcaggcgg tcgacaacgt cgatgatttc agcccgtaca cggtcgattt cgccggccag
     3061 aatctgaacg tgactatcct tcgaaacggc gaagagatcc agacgacggt cgtcccgcgc
     3121 gagaacccac cgcccgacca ggggcgtacc gggatcatcc tggccaacta ccgccttgcc
     3181 agcatcgaaa tcacgaacgt ccctgacgac agcgtcgctg ccgcggccgg gctgcaagag
     3241 ggcgaccaga tcattgccgt cggagacatg ccggcaactg actacatggc ctatgccggc
     3301 gtcattcgca accatgccgg ccagacggtt gacgtgacgg tccagcgcga cggcgaacgg
     3361 gtcgtcgttc cagttgccgt cccggttaat gtgcccgcca acgatccacc gctgggcgaa
     3421 gacgtcgtcc agaacctcaa gttcgagcga attcccatcg cggaggttcc gggcgaatcc
     3481 gtcgatctgt ttttcggctc gatcaagcag atgggccagg gactggtcag cctcattcgc
     3541 ggtgagacgc cgctcgatga tctggccgga ccgatcggga tgggacagct cacgtcggaa
     3601 gtcgtcagcg agagcgccct gccgctttgg gttacgctgg ccaacctgat gttcattctg
     3661 tcgcttaacc tcggcctgct gaacctgttg ccgctgccag cgctcgacgg tggccggctc
     3721 ctctttgtgc tgatcgaggt tctacgcggt ggccgccgtg tctcgcccga aaaggaaggc
     3781 atggtccact tcgtcggcat ggtgctgctg ctgacggtca tgctcggtat cgcgttcctc
     3841 gatgtcgagc ggctgatcag cggtaacacg ttcctggagt agtgttgtcc tcgtatgctt
     3901 gaccagacgc gccgcgcctg ccacgctgat tgtcagctgg tccaacgcgc cgcggttcac
     3961 cggcgtccgc ccgccatcag gaggtagttc gtgtctctgc tcgctccgcg ccgcctcacc
     4021 aagccggtct atgtcggcga tgtccgtatc ggcggcggcg ctccaatcgt cgtccaatcg
     4081 atggcgacgg ccgatacccg cgatccgaaa gccaccctgc gccagatcag cgagctcgcc
     4141 gacgccggtt gcgagatcgt ccgcatcgcc gtcccggacc gcaaggcggc cgcggccctg
     4201 ccggatatcg tcccgcactc tcccgtgcca ctgatcgccg acatccattt cgagcacaca
     4261 ctggcgctca aggcacttga cgccggcatc cacggcctgc gcctcaaccc cggcaacatc
     4321 cgcaagcacg aggacgtccg cgaggtcgtc aagaaggcca cggagcgcaa cacgccgatc
     4381 cggatcggcg tcaacttcgg ctcggtcgca ccgatgacgc aggagttcgt tgacgagatg
     4441 gcgcagcagc acgccagcca ggtcgagctg gtcgcggagc acatggcccg cagcgcgctg
     4501 ggccacatcc agattctcga agacctcgac tacgacaaca tcaagatctc actcaaggca
     4561 tttgaggtcc cggtcatgct ggaggcatat aagcggatgg cgacccgcaa cgcttacccg
     4621 ctccacctcg gcgtcaccga agccggcaca ccacgcgccg gtagcgtccg ttcggcagta
     4681 ggcctcggca ccttgctggc gctcggcatc ggcgacacga tccgtgtctc attgacgacc
     4741 gatccggtcg aggaagtctt cgtcgcctac gagattctca agtcgctgga gctacgccag
     4801 aagggcgcga cgatggtcgc ctgcccgacc tgtggccggg tcgaggtcga tctgttcagc
     4861 atcgccaacc gcgtcgaagc cttcctggcc gagatcaacg a
//
LOCUS       DASUGJ010000719         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_673983, whole genome shotgun sequence.
ACCESSION   DASUGJ010000719 DASUGJ010000000
VERSION     DASUGJ010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673983"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..653)
                     /locus_tag="VFV93_16280"
     CDS             complement(267..653)
                     /locus_tag="VFV93_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166965.1"
                     /translation="MSGTQTAPVIDELSQEKAPDEFKRAIVSDPFANTFRFDFDAMRG
                     GPIDPATLYAGDTVVPLSGFYFDIDGEVVNWYNKGDRFPHLSPVAGRGGRFLFITDRR
                     NATTSDLRWIILRRGWEGDVKEVHVR"
     gene            741..2048
                     /locus_tag="VFV93_16285"
     CDS             741..2048
                     /locus_tag="VFV93_16285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166966.1"
                     /translation="MADVARVKRGRKSGGLYYGWVILFVSYMLMFFTAGLTQAFGVFL
                     VPMTDDFGWDRSSFALALSIFAVVSAIVPPFAGRMADRYGPRLVLTAGAALNALGMVL
                     MAFTQSLWYAYIVYGLVIGLGFGIAGQSANAALLSRWFVKRRGMALSISSTGLGMGQL
                     ILVPLATFITIHFSWRTAFLVMGVMAAVMVPVCLLLLRRDEPPENSHETQEANRPREL
                     PATGCLPSELIRQQTHAAFRSRSFWLLGGGFMGCGFTIYLLMTHVVALATDRGISAGQ
                     AGTALGLVGGTGIVSGVIVGSLSDRIGRKHLLAGLYLLRAASVLVLMGANSATELYIF
                     AVMFGLSRANGALVSAAVIDLYGRNAVGSILGYTTTFHQLFAALGAWTGGLAYDMTGS
                     YTLALAPCIALLLGGAAASLLIDEKRPQRQPAVSYQPSAAAGG"
BASE COUNT          334 a          741 c          733 g          443 t
ORIGIN      
        1 gtgatgcccg aggcgcgcgc caggcaccag gagttcacga cctgttacgc caatgacacg
       61 gcgactcgcc cgaagactgg cgaaaccgcg acgttggttg ccaccgtcca ggcgatcatc
      121 actccacggc gctagccgtt attcgacccg ttgatgctgg ctcgtctcgg cacaatcgcg
      181 ctgaacgagc gagcccggag cgcaaccggg cgctccgggc tcgcgaatga ttgaggcaag
      241 acgatcagtc cgcgctgcat cacgaatcag cggacgtgca cctctttcac atccccctcc
      301 cagccgcgac gcaggatgat ccagcgcagg tcgctggtgg tggcgttgcg gcggtcggtg
      361 atgaagagga agcgaccacc acggccggca accgggctga ggtgcgggaa gcggtcgccc
      421 ttgttgtacc agttgacgac ttcgccatcg atatcgaaat agaagccgct cagcggaacg
      481 acggtgtctc cggcatagag cgtggcgggg tcgatcgggc cgccccgcat cgcgtcgaag
      541 tcgaagcgaa acgtgttcgc gaacgggtcg ctgacgatcg cacgcttgaa ctcgtccggc
      601 gctttctcct gcgagagctc atcgatgact ggcgctgtct gtgtgccgct catggtcccc
      661 ctgcttttcc cgttcaccct cgacaatccg gccaagctcg tgttacatcc tctgcgtagt
      721 gtatccggcg gggaggattt gtggcagatg tggcgcgggt gaagcggggc aggaagtctg
      781 gcgggctgta ttacggctgg gtgatcctgt tcgtttccta tatgttgatg ttcttcacgg
      841 ccggcctgac ccaggcgttc ggcgtcttcc tggtgccgat gaccgacgac ttcggctggg
      901 accgctcgtc gttcgcgctg gcgctgtcga tcttcgccgt cgtctcggcc atcgtcccgc
      961 cattcgccgg ccggatggcc gaccgctacg gcccacggct ggtgctgacg gccggcgcgg
     1021 ccttgaacgc gctcggcatg gtcctgatgg cctttaccca gagcctctgg tacgcctaca
     1081 tcgtctacgg gctggtgatc ggcctcggct tcgggatcgc cggtcagtcg gccaacgcgg
     1141 cgctcctctc gcgctggttc gtcaagcggc gcgggatggc gctctcgatc tcctcgaccg
     1201 gactggggat gggccagctt atactcgtcc cgctggcgac cttcatcacg atccacttct
     1261 cgtggcggac ggcgttcctc gtcatgggcg tgatggcggc tgtgatggtc ccggtctgcc
     1321 tgctgctgct gcgccgggat gagccaccgg agaacagcca cgagacgcag gaggccaacc
     1381 ggccccgcga gctgccggcg accggctgcc tgccgtccga attgatccgc cagcagaccc
     1441 acgccgcctt ccgcagccgc tcgttctggc tgctcggcgg cggctttatg ggctgcggtt
     1501 ttaccattta tttactaatg acacatgttg tcgcactggc gaccgatcgg ggtatctcgg
     1561 ccgggcaggc cgggacggcg ctcgggctcg ttggcgggac cgggatcgtc tcgggcgtga
     1621 tcgtcggctc gctctccgac cggatcggtc gcaagcacct gctggccggg ctctacctgc
     1681 tgcgggcggc gtcggtgctg gtcctgatgg gcgccaacag cgcgaccgag ctgtacatct
     1741 tcgccgtcat gttcggactc tcgcgcgcaa acggggcgct ggtgtcggcg gccgtcatcg
     1801 acctctacgg gcgcaacgcg gttggctcga tcctcggcta cacgacgacc ttccaccagc
     1861 tcttcgccgc ccttggcgcc tggaccggtg gcctggccta cgacatgacc ggctcctaca
     1921 cgttggcgct ggctccctgc attgccctcc tgctgggtgg cgccgccgcc agcttgctga
     1981 tcgacgagaa gcgcccccag cggcaaccgg ctgtcagtta ccagccatcg gctgcggctg
     2041 ggggttaggc ttgcgccttc gggacgcgtc ccacgtcccg cgtcccgcgt cccgaccgct
     2101 atacttattc ccgcaaatcg catcaccgat ccaggttggg ggagagcatt atggccggac
     2161 attccaagtg ggcacagatc aagcgcgata aggcgtccaa cgattcgaag cggggagcga
     2221 tgttctccaa gctcgcccgt gaggtctatg t
//
LOCUS       DASUGJ010000720         6817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1786216, whole genome shotgun sequence.
ACCESSION   DASUGJ010000720 DASUGJ010000000
VERSION     DASUGJ010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786216"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VFV93_16290"
     CDS             <1..839
                     /locus_tag="VFV93_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5166967.1"
                     /translation="ERFRPPSRSGASPFVTQERDDRPGQTLWGHRQEALDTLIKPIVE
                     TQAVTREYPMGETVVRALRGIDMRVQRGTLVAVKGRSGSGKTTLLNLIGGLDRPTAGH
                     VYFDGREVSNMDEADLIDLRRNKIGFIFQAFGLIPILSAAENVEIPLRLARVNVAQRE
                     ERVRVLLELVGLGERAGHRPHELSGGEQQRVAIARALANAPELLLADEPTGQLDSQTG
                     RTIMTLIRALVHSEGVTAIVATHDPVLIDLADRMIELRDGSVIYDSASVADESERYSV
                     HR"
     gene            complement(821..967)
                     /locus_tag="VFV93_16295"
     CDS             complement(821..967)
                     /locus_tag="VFV93_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166968.1"
                     /translation="MRRTANPTRRGDGGSVASHDTTLNAALGCAAAVALVVVVILIVY
                     LWTL"
     gene            complement(964..2058)
                     /locus_tag="VFV93_16300"
     CDS             complement(964..2058)
                     /locus_tag="VFV93_16300"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX5166969.1"
                     /translation="MDQHEIERVYRQCDRFLGHHYPCSPKQTLLDLAATVDDDALPDT
                     YGAGELIESLEARIAELLGKEAAVFLPSGTMAQQIALRIWTTRSGIPTVAMHPRNHLD
                     AFEHHAYEILHGMRGIRTGSPNRLLTLDDLKAIPEPIGALLLELPQRELGSILPDWDD
                     LVAIAEWARSRAIPLHMDGARLWESQPFYGRSYAEICANFDSVYVSFYKTLGALSGAA
                     LAGPDDLIAEARLWQHRHGGRLIRAYPAVVSAALALDQRLDRMAAYHDKTIGIAVALR
                     DHPDIEVTPDPPHTNMLHIYLRGDRDRLIDAAIATAADTGVFLTQRLGESPRPAWQKL
                     ELTVGDAALDLPDDTIRSLFDQWLARAKQP"
     gene            complement(2142..2987)
                     /locus_tag="VFV93_16305"
     CDS             complement(2142..2987)
                     /locus_tag="VFV93_16305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5166970.1"
                     /translation="MIDNLRQRIGPFGVWHGGTPPADLAPAIERLGYGAFWVGGSPSG
                     DLQVVEDLIAATTSLIVATGIVNIWKSDADEVARSFARIQTRYPDRFVLGIGVGHPEA
                     TQEYRSPYASLEGYVDTLLAAGVPAGSLVLAALGPRVLRLAAERTAGAHPYLVPSAHS
                     RVAREVMGPAALLAPEHKVVLDQDPDHARSLARKTVSRYLGLTNYTDNLRRMGFGDDD
                     LTGSGSDALIDALVAHGSTEQVVGQLRKHLSAGADHVAIQLLRHDDEDPIESFTALAQ
                     ALRLP"
     gene            3279..5048
                     /locus_tag="VFV93_16310"
     CDS             3279..5048
                     /locus_tag="VFV93_16310"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5166971.1"
                     /translation="MPLADILEQLSEERTWRHLTHVTEQIPSRLAGSANARRMAEYTA
                     AQLGDAGLDAGMDDFLGLVSFPKPAEVQVIGPEAWTIEASTLAQSLSTPRLEGELVYI
                     GAGAEADYDGRDLHGRITLTDLPYAPARHEKAFIAARHGVIAQVMMNWGHPESEAIPL
                     GSVKSAWGNPTPETLETEMPRIPCAGISLRDGLRLKALCEAGPVRIRLRAEAENGWHP
                     MSMTKGQIGVEPGGPFLLVGGHMDSWYGPQATDNASGNACILELARVFAQHSSELRRG
                     LVTAFWMGHETGTMISSTRFADVNWDWLRRSCVAYLQIDQPAISGTSTWHLASTEDTQ
                     GWATRAAQEMLGDMDIHWRRQRKNGDSSFFGVGLSTVAGLMSFRDDEIQATGLANLGW
                     WHHSIHNTLDKVDRGLLVPHLRVYARWMWDLLTAPILPYEFGSFAGRIGERLEGLASL
                     NVPDIDMDGAVERARTFQRLANRLDALAGAWRGRADLDQHEAEAETINRALQALARTL
                     IPVTSTTAGAYGQDRYGHSWQPELIPSLAPYTRLAGDDRDSEQFQSWWVSQIRARNRV
                     ADALERAAEIAGTALSELGERHV"
     gene            6407..>6817
                     /locus_tag="VFV93_16315"
     CDS             6407..>6817
                     /locus_tag="VFV93_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166972.1"
                     /translation="MIRSFTRVARGETHQRLVVAVILVTFLFGLVGIMQMASADTETV
                     TLTVNTSAGDARSDGGFRGTERETLVGAGDGVNANVVGLYFGSTGIPKGATITSVKVS
                     MMKKKTEWSRMIFDCAFEAVDSSQPFSSSSAPIDR"
BASE COUNT         1274 a         2076 c         2281 g         1186 t
ORIGIN      
        1 acgagcgctt ccggccaccg tcccgctccg gggcatcgcc gttcgtgacc caggagcggg
       61 atgaccggcc ggggcagacg ctctggggtc accgccagga agccctggac acgctgatca
      121 agccgatcgt cgagacgcag gcggtcaccc gcgagtatcc gatgggcgag acggtcgtcc
      181 gggcgctgcg cggaatcgac atgcgcgtcc agcgcgggac gctggtggcg gtcaaaggcc
      241 gctccgggtc cggcaagacg acgctgctga acctgatcgg cggtctggac cggccgacgg
      301 cgggccatgt ctacttcgac ggccgcgagg tgtcgaacat ggacgaggcc gatctgatcg
      361 acctgcgccg caacaagatc ggcttcatct ttcaggcgtt cgggctgatc ccgatcctct
      421 cggcggccga gaacgtcgaa atccccttgc ggctggcgcg ggtgaacgtc gcccagcgcg
      481 aggagcgggt gcgggtactg ctggagctgg tcgggctggg cgagcgggcc ggtcaccggc
      541 cgcacgagct ctccggcggc gagcagcagc gcgtggcgat cgcgcgggcg ctggccaacg
      601 cgccggagct gctgctggcc gacgagccga cgggccagct cgactcgcag accgggcgaa
      661 cgatcatgac gctgattcgg gcgctggtcc acagcgaggg cgtgacggcc atcgttgcga
      721 cccacgaccc ggtcctgatc gacctggccg accgaatgat cgagctacgc gacggcagcg
      781 tcatctacga tagcgcgagc gtcgctgacg agagcgaacg ctacagcgtc cacaggtaga
      841 cgatcaggat cacgacaaca accagcgcga ctgcggcggc gcagccgagc gcagcgttga
      901 gggttgtatc gtgagaggcg acgctgccgc catcgccccg acgtgtcggg ttggccgtgc
      961 gcctcatggc tgcttggcgc gagcgagcca ttggtcgaag aggcttctga tcgtgtcgtc
     1021 gggcagatcg agcgcggcgt cgccgacggt cagttcgagc ttctgccagg ctggacgggg
     1081 gctttcgccg aggcgttggg tgaggaagac gccggtgtct gccgcggtcg cgatcgcggc
     1141 gtcgatgagc cggtcgcggt ccccgcgcag gtagatatgc agcatgttgg tgtggggcgg
     1201 atccggcgta acttcgatgt caggatgatc gcgcagggca acagcgatgc cgatcgtctt
     1261 gtcatggtag gcggccatcc ggtcgagacg ctgatcgaga gcgagcgcgg ccgagacgac
     1321 ggccggatag gcgcggatga gccgtccacc gtggcggtgc tgccagagac gggcttcggc
     1381 gatcagatcg tccgggccgg ccagggcggc accactcagc gcaccgagcg tcttatagaa
     1441 cgagacgtag acgctgtcga agttggcgca gatttcggcg tagctccgtc cgtagaacgg
     1501 ttgcgattcc caaaggcggg cgccgtccat gtggagcggg atcgcgcggg agcgcgccca
     1561 ctcggcgatc gcaaccagat cgtcccaatc gggcaggatg ctgcccagct cgcgttgcgg
     1621 cagttccagc aggagcgcgc cgatcggttc ggggatggcc ttaagatcgt cgagcgtcag
     1681 cagacgattg ggcgagccgg tacgaatgcc gcgcatgcca tgcaggatct cgtaggcgtg
     1741 gtgctcgaag gcgtcgaggt ggttgcgcgg gtgcatcgcg acggtcggga taccggagcg
     1801 ggtcgtccag atccgtagcg cgatctgctg cgccatcgtg ccgctgggca ggaagacggc
     1861 cgcctccttg ccgagcagct ccgcgatacg tgcttcgagc gactcgatca gttccccggc
     1921 gccgtaggta tccggcaagg cgtcatcgtc aacggtggct gccaggtcga gcagggtctg
     1981 cttcggcgag cacgggtagt ggtggccgag gaatcggtcg cactggcggt agacgcgttc
     2041 gatctcgtgc tggtccatct gtcctccgcg tgatgcgtga tgcgtggggg tagcgtatca
     2101 cgcattcggg ttgatcatgc tccatcggtg cgctccgcga ttcacgggag tcgcagagcc
     2161 tgcgcgaggg ccgtgaagct ctcgatcgga tcctcgtcgt catggcgcaa aagctggatg
     2221 gcgacatgat cggcgccggc tgacaggtgc ttgcggagct gcccgacaac ctgctccgtg
     2281 ctgccgtggg cgacgagcgc gtcaatcagg gcatcgctgc cgctgccggt gaggtcgtcg
     2341 tcgccaaagc ccatgcgacg caggttgtcg gtgtaattcg tcaaacccag gtaccgggag
     2401 acggtcttgc gcgccagcga ccgggcatgg tcggggtcct ggtccagcac gaccttgtgt
     2461 tccggcgcga gcagggcggc cggccccatc acctcgcggg cgaccctgga gtgcgctgac
     2521 gggacgaggt aggggtgtgc gccggctgtc cgttcggccg ccagccgcag gacgcgcggg
     2581 ccgagcgcgg ccaggaccag gctgccagca ggcacgcccg cagcgaggag cgtatcgaca
     2641 taaccctcca gcgaggcgta cggtgagcgg tactcctggg tcgcctcggg atgtccgacc
     2701 cctataccga ggacaaaccg gtcggggtaa cgagtctgaa tacgggcgaa cgagcgtgcg
     2761 acctcgtctg cgtcactctt ccagatattg acgatgccgg tggcgacgat cagtgaggtc
     2821 gtcgcggcga tcagatcctc caccacctgc agatcaccgc tgggcgaccc tcccacccag
     2881 aaggcgccgt agccgagccg ctcgatggca ggcgcgaggt cagccggcgg cgtaccaccg
     2941 tgccagactc cgaacggacc aatgcgctga cgaagattgt cgatcatgcg ttgtccttat
     3001 tccattgttt gtcatcctga ggcgtgagcc gaaggatctc tccacgggac acggatcggc
     3061 tggtgacgga gagatccttc ggctcacgcc tcaggatgac acagcggggg cggcaggaga
     3121 tggctcctga cgacagcgta tgcctgtccc gcgcgcatcg tcaagttgga cacgccaacg
     3181 gaccatcgct ctcgattggc tgcgattcgt gttacagatc ccgccgtgtt tgctagcatg
     3241 gcgatatcgg agagacgaac aggcgggagg atgattcaat gccacttgcc gacattctcg
     3301 agcaactgtc cgaagagcgc acctggcgtc atctgacgca cgtcaccgaa cagatcccgt
     3361 cgcggctggc cgggtcggca aacgcccgcc ggatggccga gtacacggcc gcacaactcg
     3421 gggatgccgg cctcgacgca gggatggacg actttctcgg gctggtgtcg tttccaaagc
     3481 cggccgaagt gcaggttatc ggtcccgaag catggacgat cgaggcaagc acgttggccc
     3541 agtccctatc gaccccgagg ctggaagggg aattggtcta tatcggcgcc ggggccgagg
     3601 cggactacga cggtcgcgac ctgcacggca ggatcacgct gaccgatctg ccctacgccc
     3661 cggctcggca cgagaaggcg ttcatcgccg cgcggcacgg cgtcatcgct caggtgatga
     3721 tgaactgggg gcatccggag agcgaggcga ttccgctcgg atcggtgaaa tcggcctggg
     3781 gcaatccgac gcccgaaacg ctcgagacgg agatgccgcg tatcccctgc gccggcatct
     3841 cgctgcggga cggcctacgg ctgaaggccc tctgcgaggc cggcccagtg cgtatccggc
     3901 tgcgggccga ggcagagaac ggctggcatc caatgtcgat gacgaagggc cagatcggcg
     3961 tcgaaccggg cggtccattc ctactggtcg gtggtcacat ggactcctgg tacggtccgc
     4021 aggcgaccga caacgcgtcg ggcaacgcct gcatcctcga actggcacgc gtcttcgccc
     4081 agcacagctc cgaactacga cgcgggctgg tgaccgcctt ctggatgggc cacgagacgg
     4141 ggacgatgat ctcctcaacc cgcttcgccg acgtgaactg ggactggttg cgccgctcat
     4201 gcgtcgccta cctgcagatc gaccagccgg ccatcagcgg cacctcgacc tggcacctgg
     4261 cgtcgaccga ggacacgcag ggttgggcaa cccgcgccgc ccaggagatg ctcggcgaca
     4321 tggacatcca ctggcgccgg cagcgcaaga acggcgattc gtcgttcttc ggtgtcgggc
     4381 tgtcaacagt cgccggactg atgtccttcc gcgacgacga gatacaggcg accggactcg
     4441 ccaatctcgg ctggtggcat cactcgattc acaacacgct cgacaaggtc gatcgagggc
     4501 tgctcgtgcc gcacctgcgg gtctacgcgc gctggatgtg ggacctcctg acggcgccga
     4561 tcctgccgta cgagttcggg agcttcgccg gcaggatcgg cgagcgtctt gaaggactgg
     4621 cgtcgctcaa tgtcccggat atcgacatgg acggggctgt cgagcgggcg agaacgttcc
     4681 agcgcctcgc caaccggctg gacgcactcg cgggagcatg gcgcggtcgc gccgatctcg
     4741 accaacacga ggccgaagcc gagacgatca accgcgcgtt gcaggcgctg gcacggacgc
     4801 tcatcccggt cacctcgacg accgccggcg cgtacgggca ggatcgttac ggtcactcat
     4861 ggcagcccga gctgatccca tcactcgcgc cctacacgcg cctggccggc gacgatcgtg
     4921 actccgagca gttccagtcc tggtgggtct cgcagatccg cgcccgcaac cgcgtcgctg
     4981 acgcgctcga gcgggctgct gagatcgcag gcactgcact cagcgaactg ggagagcgac
     5041 acgtctgagg ggcaataccg agcagcgact atggtacccg gcgcagcatc gtaaccgcct
     5101 gcaccggtga tccacgcgtg acgtcgatgc gggttgtaga ccgtttcgcc gttgtcagac
     5161 atcactaccc gggcatccgc atgggtccgg aagtttagcc ggcgcagcga atactgagtc
     5221 tcgccggctg gcggaacggg ctgattgata ctcagatccg caatgaacac gccgcgcttc
     5281 tcggcttcag ggatcacgaa agagggcgcc ttgagccatg gtacaacgtt gggatgaacc
     5341 acctggtata ccgatcaaat cgtcctattg cacctgcttc catccaaacc gatagacacg
     5401 actagcattt ttgcgatgtg aagagagaca atttgagtat tgagcgatag aacgaatcga
     5461 tttgacacga tgtcaacaca cgcatatgtg ccatgggtga cagacataga gaagcgcatc
     5521 gatataggtt caatcaatgg aagcaatact ccgctcattg gcggccattt gatgttaaat
     5581 attgcaatct gatggtgaca aactattcac aacgtgatat cgtacggcga tcacggagaa
     5641 tgtccgtgtt ggatgaggac gcaggagaga cattccaaac aggtatggcc acgtcgcgag
     5701 aaactaactg cagtggggtg cacgttggtc ggacgtgaat ccgcatcctc ctcatccatg
     5761 aaggaaacgg gtgaacggtg acgttcctct ccgtaaggga cggagagttg tgcaacgttc
     5821 aacgagacgc ctgactgctt cgccttcggg agggcgggga acccttgcgg ggcagcagcg
     5881 cgcgggaaag ggaatgtcgt ggtacaacgt cgttgcgtgc atcacccggc atgtcgatgc
     5941 acactacgcc aagttgtcgt acgtctcgag tacacacatc ttccattgaa gataccagat
     6001 tcctcccgac accgatcgtc cggggatggc tcaaccaaca cttcatctcc ttcgggtaca
     6061 tcacagatga aagacacgcc tgcctgacgc gcttcgcatc gttccacgat ggcaggaagt
     6121 agcgtcgtgc ctctgtttct gacttcggac atgaagcagc caggcccgac ccgttcagag
     6181 cacggacaga gtcgacacgg tatgcgctgg tgaaggcacc ggggcgtgct tcgttgcgct
     6241 tcgatgcatg gctcatgcag ccgacgcagc atcccgctgg tcctatgtgg catcaccccg
     6301 aacgggctag atcgccccgt actgataggt gaatgaacct accacaggtt gatgcaacta
     6361 tacgaccgct gtttcgtgct ttcatcgaca ggaaggaccg tacgaagtga ttcggtcatt
     6421 cacacgtgtt gcacggggag agacccacca acgtctggtc gttgcggtca ttctcgttac
     6481 atttctcttc gggttggtcg ggatcatgca aatggcctcg gccgacacgg agaccgttac
     6541 cttgacggtc aacacgtccg caggtgacgc ccgttcggat ggcggattca gaggcactga
     6601 gcgcgaaacg ctcgtcggcg cgggcgatgg ggtcaacgcg aacgtcgttg gcctgtactt
     6661 cggctcaacc ggcatcccca agggggccac gatcacctca gtcaaggtct cgatgatgaa
     6721 gaagaagact gagtggagcc gaatgatctt cgactgtgcg ttcgaggcag tcgatagttc
     6781 gcagccgttc tcgtcgagtt ccgcgccgat cgatcgc
//
LOCUS       DASUGJ010000721         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_953128, whole genome shotgun sequence.
ACCESSION   DASUGJ010000721 DASUGJ010000000
VERSION     DASUGJ010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953128"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFV93_16320"
     CDS             complement(<1..703)
                     /locus_tag="VFV93_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5166973.1"
                     /translation="MTTTTAATVLVESDSASRIATVTLNRPEKRNALSLALMRELTAT
                     LREIGARRDIAVVILTGNGPAFSAGHDISEMTGREIDDYREIFDVCCELMETVQSIPQ
                     PVIAMVRGIATAAGCQLAATCDLVLCEESSRFGTPGVKIGLFCSTPMVAVSRVIGPKR
                     AMQMLLTGVPIDARTAADWGLVNEVVPAEQLETRTREVAAQIANASSTVIGIGKEAFY
                     AQIDRDQHAAYDYTKT"
     gene            825..>2057
                     /locus_tag="VFV93_16325"
     CDS             825..>2057
                     /locus_tag="VFV93_16325"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5166974.1"
                     /translation="MMTTPLLQPVISPILVGRDEYLAALDSLASGVKAGQGVTLLVSG
                     EAGIGKSRLVAEARTRFRREPAGRVLQGHCFEHDAGLPYAPFVDLFHNMLAEQPDELS
                     RLLAVAPELAVLLPELADQPVPSTTADAEQDKRRMQQALVRMVATLSGEQPVLLIIED
                     IHWADGLTLELLQRLAQRVAALRLLLIVTWRDEEESEPLRHFLAAVDRGRTGRELQLA
                     RLEPAAVERMLRAMFGQPQPIRPDFLETIVGLTDGNPFFIEEVVTALIASGDIYRENG
                     NWSRKELTELRIPRSVVDAVQQRARGLSGTARQLLDLAAVAGQRFDVGIVQEVLVLDD
                     AALLGAIRELVEARLIVDVSDEQAAFRHALTREAVYAALLARERRAHHLALAEAIERR
                     HAASPGPYAVDLSYHYHAA"
BASE COUNT          331 a          665 c          712 g          349 t
ORIGIN      
        1 ccgtcttggt gtagtcgtag gcggcgtgct ggtcgcggtc gatctgggcg tagaaggctt
       61 ccttgccgat gccgatgacg gtgctgctgg cgttcgcgat ctgggcagcc acctcgcggg
      121 tgcgggtttc gagctgttcg gccgggacaa cctcgttgac aagaccccaa tcggcggccg
      181 tccgggcgtc gatcgggaca ccggtcagca gcatctgcat ggctcgcttc ggaccgatga
      241 cgcggctgac ggcgaccatc ggcgtcgagc agaacaggcc gatcttgaca ccgggtgttc
      301 cgaatcggga ggactcctcg cagaggacga ggtcgcaggt cgcggccagc tggcagccgg
      361 cggcagtcgc gatcccgcgc accatcgcga tgaccggctg gggaatcgac tggaccgtct
      421 ccatcaactc gcagcaaaca tcgaagatct cgcggtagtc gtcgatctcg cggccggtca
      481 tctccgagat gtcgtggccg gccgagaagg ctgggccgtt gccggtcagg atcacgacgg
      541 cgatgtcacg tcgcgcgccg atctcccgca aggtcgcggt cagttcgcgc atcagcgcca
      601 gcgagagggc gttgcgcttt tcgggccggt tgagggtgac ggtcgcgatc cgcgaggcgc
      661 tatcagactc gacgaggacg gttgcggcgg ttgtcgttgt cattggggaa cccttccacc
      721 ggcgccggag ccggagcgtg atctgcgggc tgggccgtat cgtaacaccg gcggcatgct
      781 aggatgggcg cgcgcaacgc cgcctcagca cagccggaac gcgaatgatg acgacaccgc
      841 tgttgcaacc ggtcatctca ccaatcctcg tcggtcggga cgagtacctc gccgcgctcg
      901 attcgttggc atccggcgtc aaggccggcc agggtgtgac gctgctcgtt agcggcgagg
      961 caggcatcgg caagtcgcgg ctggttgccg aggcacgaac ccgcttcagg cgggagcccg
     1021 ccggtcgagt tctccaggga cactgcttcg agcacgacgc cgggcttccc tacgcgccgt
     1081 tcgtcgatct cttccacaac atgctggcgg aacagccgga cgagctgtcg cggctgctgg
     1141 cagtcgcacc agagctggcc gtgctgctgc cggaactggc ggaccagccc gtgccttcaa
     1201 ccacggccga cgccgagcag gataagcgcc gcatgcagca ggcactggtg cgcatggtcg
     1261 cgacactgtc cggtgagcag cctgtgctgc tgatcatcga ggacatccac tgggcggacg
     1321 gtctcacact cgagctgctc cagcggctgg cgcagcgagt cgccgctctg cggctgctac
     1381 tgatcgtcac ctggcgcgat gaagaagagt cggagccgtt gcgccacttc ctggcggcag
     1441 tcgatcgggg tcgaaccggc cgcgagctgc agttggcgcg gttggaaccg gccgccgtcg
     1501 aacggatgct gcgagcgatg ttcggccagc cacaaccgat ccggcctgat ttcctggaga
     1561 cgatcgtcgg cctcaccgac ggcaatccgt tcttcatcga ggaggtcgtc accgcgctga
     1621 tcgccagcgg cgacatctac cgcgaaaacg gcaactggag ccgcaaggag ctgaccgagc
     1681 tgcgtattcc gcgcagcgtc gtcgatgccg tccagcagcg cgcacgtggc ttgagcggca
     1741 cagcccgcca gctgctcgac ctggctgccg tggcaggaca gcgattcgat gtcggcatcg
     1801 ttcaggaggt gctggtgctc gacgatgcgg cgttgcttgg cgcgatccgg gaactggtgg
     1861 aggcgcggct gatcgtcgac gtgtcggacg agcaggctgc cttccgccac gccttgacgc
     1921 gcgaggccgt ctatgcggcg ttgctggcgc gcgaacggcg cgcgcaccac ctcgcactgg
     1981 ccgaagcgat cgagcgcaga catgcagcgt cgccgggacc ctacgctgtc gatctctcct
     2041 accactacca cgctgcc
//
LOCUS       DASUGJ010000722         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1230062, whole genome shotgun sequence.
ACCESSION   DASUGJ010000722 DASUGJ010000000
VERSION     DASUGJ010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230062"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFV93_16330"
     CDS             complement(<1..614)
                     /locus_tag="VFV93_16330"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166975.1"
                     /translation="MTLPQIETRKGDVVEDYHGTPIADPYRWLEDPADPEVVAWTEAQ
                     NRRTRAFLDAIPARKRIEGRLTRLWDYPRWSIPEKEAGRAFFFKNDGLQNQAVLYVTL
                     APDADPVELLDPNALSEDGTVAVTAVSASDDGRLLGYALAEAGSDWQRLRVRDVDERD
                     DLPDLIRWCKFSPIAWTRDSAGFFYSRFPEPGTVAAEDANNYNT"
     gene            complement(631..1161)
                     /locus_tag="VFV93_16335"
     CDS             complement(631..1161)
                     /locus_tag="VFV93_16335"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX5166976.1"
                     /translation="MPVTKIGAIVASSGGDAPAATTTPGRTQMTRRNISSGSPLEGPI
                     GFSRAVRLGQYIAVAGTAPIALDGGTTAPGDVEGQTRRCIEIAAEALAEAGASLADVI
                     RTRIMLTNIDDWQAAARAHGAAFGDIRPACTFVQVSRFIDPEWLVEVEMDAVVERDSG
                     IVNAARRSRIPCPESR"
     gene            1263..2708
                     /locus_tag="VFV93_16340"
     CDS             1263..2708
                     /locus_tag="VFV93_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166977.1"
                     /translation="MTDTDRSGGWLGDNAGSVFAWLGAATLLAYAVFWLRPLWLGRFE
                     PFAGGALYIGTIFGYSLSGFNQFALAALVPSLLYLAALLLLSKVPLREAMPVIAGVTV
                     IAPIVLLTTYPALAADVFGYLMYGRMISAHGGNPYIDTANMFPHDIYFPPVGGDWRDY
                     PAPYGPVWVWLSALVTWLAGGHQLAALLALKLIAIAAHLGSAALVWQLTREISGSERR
                     AAWALLAYGWNPLTITHFALDAHNDALMVLFLLAAIWLMRRCRPDLAFPALVLAVLVK
                     FVPVLLAPLFLLAARHQPRRALLGIVAAFGLAVLAYAPLWDGIDTFDAVRKQSDLRTS
                     SPLTLLHFYVNGDWLRPLSLAVFGLGYLLILWRVRSLEGRSYAVIVLYYFALSSWTKG
                     WYFTWALALGAVLGGPAFAAAGLASFGVFLQNMTAWGWEMDTFHWASRYGVHFWEWWL
                     TWTLYLPWLLVAAWVLVKHFVCGRYRVIRDA"
     gene            complement(2807..3403)
                     /locus_tag="VFV93_16345"
     CDS             complement(2807..3403)
                     /locus_tag="VFV93_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166978.1"
                     /translation="MRTIPMVAGGLAHAIEILRDDELPVMRQAAGFHGMIVLASLSDS
                     AAGERVEIVSLWESQQHLDTFDAADQRRQTVQTMPQLSAPAQLRVYEVAQFAGIPGGT
                     VSRFISVQLNPGYIDTVVGLFENVVMHAATEQHGFRRGLLLVDRTANRAISIGLWHSE
                     ADLIASERTGYLSQQIGNFTQVVAAPIVPETLMVAVEC"
BASE COUNT          488 a         1169 c         1212 g          680 t
ORIGIN      
        1 atcgtgttgt agttgttggc gtcctcggcg gcgaccgtgc ccggctccgg gaagcgcgag
       61 tagaagaaac cggcgctgtc gcgggtccag gcgattggcg agaacttgca ccagcggatc
      121 aggtccggca ggtcgtcgcg ctcgtcgaca tcgcgcaccc gcagccgctg ccagtcgctg
      181 ccggcctccg ccagcgcgta gcccagcagc cgcccgtcgt cggaggctga gaccgccgtc
      241 accgcgaccg tgccgtcctc ggagagcgcg ttcgggtcga gcaactcgac cgggtcggcg
      301 tccggtgcaa gcgtgacgta gaggacggcc tggttctgga ggccgtcgtt cttgaagaag
      361 aaggcgcggc cggcctcctt ctccgggatc gaccagcgcg gatagtccca cagccgcgtc
      421 agccgcccct cgatccgctt gcgggccggg atcgcgtcca gaaacgcgcg tgtccgcctg
      481 ttctgcgcct cggtccaggc gaccacctcc gggtcagccg ggtcctcaag ccaccggtac
      541 ggatcagcga tcggcgtccc gtggtagtcc tcgacgacat cgcctttacg tgtctcgatc
      601 tgggggagcg tcatggattt gtcctttcta tcaccgggat tcgggacatg ggattcggga
      661 tcggcgggct gcgttcacta ttcccgaatc ccgttctacg accgcatcca tctccacctc
      721 aaccagccac tctggatcga tgaagcggga gacctggacg aaggtgcagg cggggcggat
      781 gtcgccgaaa gcggcgccgt gggcgcgggc ggctgcctgc cagtcgtcga tattggtcag
      841 catgatgcgg gtgcggatga catcggcgag actggctccg gcctcggcca gcgcctcggc
      901 ggcgatctcg atgcagcggc gggtctgccc ttcgacatcg ccaggtgcgg tcgtgccgcc
      961 gtccagcgcg attggcgcgg ttcccgctac cgcaatgtat tgcccgagcc ggacggcccg
     1021 tgagaagcca atcggcccct caagtggcga gccggatgag atattgcgtc gcgtcatctg
     1081 cgtccttccc ggcgtggtgg ttgcggctgg cgcgtcgccg ccggagcttg cgacgatagc
     1141 gccaatcttc gttaccggca agtccgtacc tgcttatgac agcggcggtt ggcgtcgtcg
     1201 cggatgagca gcgcgtggca cactccgcgc cggtgcgtgc ggccgtcagg ctgggggcat
     1261 gtgtgacgga taccgaccgg tcggggggct ggctcggcga caacgcgggg agtgtcttcg
     1321 cgtggcttgg ggccgcgacg ctgctggcgt atgcggtctt ctggctccgg ccgctctggc
     1381 tgggccgctt cgagccgttc gcgggcggcg cgctctacat cggcacgatc ttcggctaca
     1441 gcctctcagg cttcaaccag ttcgcgctgg ccgcgctcgt gccgtcgctg ctctacctgg
     1501 cggcgttgct gctgctgtcg aaggtgccgc tacgcgaggc gatgccggtc atcgccggcg
     1561 tgaccgtcat cgcgccgatc gtgctgctga cgacctaccc ggccctggcg gccgacgtct
     1621 tcggctacct gatgtacggg cggatgatca gcgcgcacgg cggcaacccc tacatcgaca
     1681 ccgccaacat gtttccgcac gatatctact ttccgccggt tggcggcgac tggcgcgatt
     1741 acccggcgcc gtatgggccg gtctgggtct ggctctcggc gctggtcacc tggctggccg
     1801 gtggccatca gctggcggca ctgctggcgc tgaagctaat cgcgatcgct gcccatctcg
     1861 gatcggccgc cctcgtctgg caactgacgc gggagatcag cggctccgag cgccgcgcgg
     1921 cctgggcgct gctggcctat ggctggaacc cgctcacgat cacccacttc gcgctggatg
     1981 cccacaacga cgcgctgatg gtgctgtttc tgctggccgc gatctggctg atgcgccgct
     2041 gccggccgga tctggcgttc ccggcgctgg tgttggcggt gctggtcaag ttcgtgccag
     2101 tgctgctggc gccgctcttc ttgctggccg ctcgccatca gccgcgccgg gcgctgcttg
     2161 ggatcgtcgc cgcgttcggg ctggccgtgc tggcctacgc gccgctctgg gatggcatcg
     2221 acaccttcga cgccgttcgc aagcagtccg acctgcggac ctcgtcgccg ttgacactgc
     2281 tgcactttta cgtcaacggc gactggctgc ggccactctc gctggccgtc ttcgggctgg
     2341 gctatctgct gatcctctgg cgcgtgcgct cgctcgaagg acgctcgtat gccgtgatcg
     2401 tcctctacta cttcgcgctc tcgtcgtgga cgaagggctg gtacttcacc tgggcgctgg
     2461 cgcttggcgc cgtcctcggc ggccccgcct tcgcggcggc cgggctggcg agcttcggcg
     2521 tcttcctcca gaacatgact gcctggggct gggagatgga caccttccac tgggccagcc
     2581 gctacggcgt ccacttctgg gaatggtggc tgacctggac gctctacctg ccctggctgc
     2641 tggtcgcggc ctgggttctc gtcaagcact tcgtgtgtgg caggtatcga gtaattcgtg
     2701 acgcgtgacg cgtgacgcgg gacgcgggac gcgggatggg gcatgaggga tgagggatga
     2761 gaaggacgct tgaccatccc cgcgtcacgc gtcacgcgtc acgcgctcag cactccaccg
     2821 cgaccatcag cgtctctggg acgattgggg cggcgacgac ctgggtgaag ttgccgatct
     2881 gctggctgag gtagccggtt cgctcgctgg cgatcaggtc ggcttccgag tgccagagac
     2941 cgatcgaaat ggcccggttc gcggtgcgat cgaccagcag cagcccgcgc cggaagccgt
     3001 gctgttccgt tgcggcgtgc atgacgacat tctcgaacag gccgacaacc gtatcgatgt
     3061 agcctggatt cagttgaacc gagatgaagc gcgagacggt cccgcccggt atcccggcaa
     3121 actgggcaac ctcgtagaca cgcaactggg ccggagccga cagttgcggc atcgtctgga
     3181 cggtctggcg gcgctggtcg gcggcgtcga acgtgtcgag atgctgctgg ctctcccaga
     3241 gcgagacgat ctcgacccgc tctccggcgg cggagtcgga gaggctggcc agcacgatca
     3301 tcccgtggaa gccggctgcc tgtcgcatga cgggtagctc gtcgtcgcgc aggatctcga
     3361 tcgcgtgcgc cagcccgccg gcgaccatcg gaatcgttct cactcgtgca atcatcgcag
     3421 gttgctgctc cctggctcgc cgaacgttcg ctgattttac tcgcgttaat gcttgtcgcg
     3481 aagcatactc gctgtcggca agagacatag cctgtcgtcc tgagccgcag cgaaggacct
     3541 cccctgcct
//
LOCUS       DASUGJ010000723         4440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_117705, whole genome shotgun sequence.
ACCESSION   DASUGJ010000723 DASUGJ010000000
VERSION     DASUGJ010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117705"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..577
                     /locus_tag="VFV93_16350"
     CDS             56..577
                     /locus_tag="VFV93_16350"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX5166979.1"
                     /translation="MRRFFWLLVLVALLTGGCDVEMASESSTARSGNGSDENVRVVRV
                     VDGDTISVEIDGREERLRYIGIDTPESVQPNTPVECFGIEASEENKRLVEGKRVVLER
                     DVSNRDRYDRLLRYVYVVDGSTRTFVNQALVEGGFAFASSFPPDVKYQDQLRAAQRKA
                     RDEGRGLWGACTP"
     gene            complement(805..1053)
                     /locus_tag="VFV93_16355"
     CDS             complement(805..1053)
                     /locus_tag="VFV93_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166980.1"
                     /translation="MAVRTRVSSVRAGALGLDADEAFRRLGVPTREAPLLGRALRNDE
                     AREVQASARRITAILLLEPALDANYTAVTDATYAWPGR"
     gene            1137..2984
                     /gene="sulP"
                     /locus_tag="VFV93_16360"
     CDS             1137..2984
                     /gene="sulP"
                     /locus_tag="VFV93_16360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEX5166981.1"
                     /translation="MLYTPRAISHSGCSGGRYRRHDAVPAGWAMPSARTKVPEQIIER
                     WMPGVAVIRQYRRSWLRGDLVAGLVLAALLVPQGMAYAKLAGLPAVYGLYATMVPLLV
                     YAIFGPSRILVLGPDSAVAPVVATAIIPLAGNDTSERVALAGFLAMLVGLVCIVAGIA
                     NLGFITDLISKPVRIGYLAGIAVSVVASQLPALLGIDVSGSGLADDLTGLARNLDSID
                     LPSLVVGGGSLAFILAMRRIEPRVPAALIVVVVTILAVQWLDLSVETVGSIPSGLPEF
                     SIPSPNSTSDLGRLALTALALALLSFADTSVLSRSYASRTGDHVDPDQELVALGAANL
                     ATGLFQGFPISSSSSRTPVAESAGAKTQLTGVVGAASLAVVLVFANGLFADLPSTVLA
                     AIVIAAMVSLVDLAGLRRLSLVHRSDFLLALTCFGSVAIVGVLWGIGIAIGLSVLAFL
                     WRAWHPYDAVLGRADSVKGYHDLTRYPEARQIPGLLLYRFDAPLFFANANIFRERLVA
                     AVQSSPQPIKRVVIAAEPITDLDSTAADVLAALDRELEMQGIDLAFAEMKDPVKDRLR
                     RFGLVDRIGRERFYPTIGVAVRRFVEEYEVDWVDWEDTVDQASSVSASN"
     gene            3125..4417
                     /locus_tag="VFV93_16365"
     CDS             3125..4417
                     /locus_tag="VFV93_16365"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5166982.1"
                     /translation="MSEHAVRVNRRGPVARMNRFYGNNGPLVWVCILIAVNQLGFGSI
                     VPILPLYAESFGVSKAAIGLTIAIYGLARFLMNVPTGYVSDRYGRRVALAIGGAITVF
                     GNFVCAVAGSYWLFLGGRFVAGAGAAMVITGTQIIVADISKPANRGRLMSIYMGTFMF
                     AVGFGPLPGGLLGEHVSLAAPFWVYCFLGGILTILAWTKMPETRGWREDGSLQAQAPS
                     LPLGQQLRLLTSQLGFTLIAIVSFCTFFARTGGLFNLIPTLAEDKIGLTSGQIGAGLA
                     MISLMGLLLAYPSGVMVDRYGRKWVIVLSTVISGFSLIAFAQAPNFLWFIVACSMWAT
                     SSGIASAAPSAYAADMAPPGMNAPAMSMYRMLADFGYVAGPLILGSAADVFATETALY
                     ATSAMVIVAGLAFAWLAPESFRREPAATPAIAGGSGRG"
BASE COUNT          673 a         1484 c         1443 g          840 t
ORIGIN      
        1 ttcaaatccc acgtcccgcg tcccgcgtcc cgaatccgaa acaacaggga gaccgatgcg
       61 acgatttttc tggctcctgg tgctggttgc gctgctgacc ggtggttgcg atgttgagat
      121 ggcgtcggag tccagcaccg ccaggagcgg caacggcagc gacgagaacg tgcgggtcgt
      181 gcgggtggtc gatggcgaca cgatcagcgt cgagatcgac gggcgcgagg agcgcttgcg
      241 ctatatcggg atcgacacgc ccgagtcggt ccagccgaat acgccggtcg agtgcttcgg
      301 gattgaggcc agcgaagaga acaagcggct ggtcgagggc aagcgcgtcg tcctggagcg
      361 cgatgtcagc aaccgcgacc gctacgaccg gctgctgcgc tacgtctacg tcgtcgatgg
      421 cagcacgcgc acgttcgtca accaggcgct ggtcgaaggc ggcttcgcct ttgcctcctc
      481 cttcccgccc gacgtgaaat accaggacca gctccgcgcc gcccagcgca aagcccgcga
      541 cgagggacgc ggcctgtggg gcgcgtgtac cccgtgacat gaagacccgc agtgtggcga
      601 gttcaaaggg aagcaggtta aaggggatga acccatccat acacaggtca ccccctttac
      661 ttctttggcc ggcgagggag agcagaatca agttcgtcgc ctgcgaatac tcagatatat
      721 atagatttac tggtatcgtg ggcgaaagaa gtaaaggggg tgacctgtgt atggatgggt
      781 tcatcccctt tgaaatgatc ccctttaccg gcctggccag gcgtacgtcg cgtccgtcac
      841 ggcggtgtag ttggcgtcga gagctggttc gaggagcagg atggccgtga tccggcgtgc
      901 gctagcctgg acctcgcggg cttcgtcgtt cctgagtgcg cggcccagca gcggcgcctc
      961 acgggtcggc acgcccagcc tccggaaggc ttcgtcggcg tcgaggccca acgcaccggc
     1021 ccgcaccgac gacacgcgcg tcctgacggc cacgatcgac ctggttggcg acagcgactg
     1081 gccgggactc gcgcacgtgt tccgggcgga atacgaccgt cgcttgaacc cccttggtgc
     1141 tttacacgcc gagggcaatt tctcattctg gttgttccgg tggccggtat agacgccacg
     1201 acgcggtgcc ggcggggtgg gcgatgccga gcgcacggac gaaagtgcca gagcagatca
     1261 tcgagcgctg gatgcccggc gtcgcggtca tccggcagta ccggcgatcg tggctgcgcg
     1321 gcgatctcgt cgccgggctc gtgctggctg cgctgctggt tccccagggc atggcctacg
     1381 cgaaactcgc gggccttccc gccgtttacg gtctgtatgc cacgatggtg ccattgctcg
     1441 tctacgcgat ctttggccca tcgcgcatcc tggtgctcgg gccggattcc gccgtcgccc
     1501 cggtcgtcgc caccgcgatc atccccctcg ccggcaatga caccagtgag cgcgtcgccc
     1561 tggctgggtt cctcgcgatg ctcgtcggtc tggtctgcat cgtcgccggc atcgcaaacc
     1621 tgggattcat caccgacctc atttccaagc cggtgcggat cggctacctt gccggcatcg
     1681 ccgtcagcgt cgtcgccagc caactcccgg ccctgctcgg catcgatgtc tcagggagtg
     1741 gtctcgcgga cgacctgacc ggactggcgc gcaacctgga cagcatcgat ctcccatccc
     1801 tcgtcgtcgg gggaggctcg ctcgccttca tcctggccat gcgacggatc gagccgcgcg
     1861 ttccggctgc tctgatcgtc gtggtggtca ccatcctcgc ggtgcagtgg ctcgatctct
     1921 cggtcgaaac ggtgggctcg atccctagcg gcttgccgga gttttcgatt cccagcccga
     1981 actcgacgag cgacctcggc aggctggcgc tcactgccct cgcgctggcg ctgctctcct
     2041 tcgccgacac gagcgtgctg tctcgcagct acgcctcccg gaccggcgac catgtcgacc
     2101 ctgaccagga gctcgtggcg ctaggcgcgg cgaacctggc aacggggctg ttccagggat
     2161 tcccaatctc cagtagctcg tcgcgcacgc cggtcgccga atcggccggg gcgaaaaccc
     2221 aactcaccgg cgtcgtcggc gccgccagcc tcgcggtggt cctcgtcttc gccaacggcc
     2281 tcttcgccga tctgccctcc accgttctgg cagccatcgt gatcgccgcg atggtcagcc
     2341 tggtcgatct agccggtctt cgcaggctgt cgctcgtcca ccgctccgac ttcctcctcg
     2401 cgttgacatg cttcgggagc gttgccatcg tcggcgtcct gtggggcatc gggatcgcga
     2461 ttggcctctc agtgctggcc ttcctgtggc gcgcgtggca tccctacgac gccgtgcttg
     2521 gccgcgcaga cagcgtcaag ggctaccacg atctcacccg atatccggag gccagacaga
     2581 taccggggct gctgctctat cgcttcgatg ccccgttgtt cttcgcgaat gccaacatct
     2641 tccgggagcg gctggtggct gccgtgcaga gcagccccca accaatcaag cgggtcgtta
     2701 tcgcggccga accgatcacc gacctggact cgactgctgc cgacgtgctc gcagccctcg
     2761 accgcgaact cgagatgcag ggcatcgatc tggccttcgc cgagatgaaa gatcccgtca
     2821 aggatcgcct gcgtcgcttc ggcctggtcg accgcatcgg acgcgagcgt ttctatccaa
     2881 ccatcggggt cgcggtgcgc cgctttgtcg aagagtacga ggtcgattgg gtggactggg
     2941 aggacacggt ggatcaagcg tcctcagtca gcgcgtcgaa ctaaccagtc gcccggcgac
     3001 aatcaaacag cgccgcgcag agccaccatt ggtatcgttc aggcggtcac cgagcgtcgt
     3061 ttcctgttcc ctttcgcccc ggcacgcgat atgctcccgc gcgtggtgag gagacaccga
     3121 tcgtgtgagt gaacatgctg tacgggtgaa ccggcggggg ccggtcgccc ggatgaaccg
     3181 gttctacggc aacaacgggc cactggtctg ggtctgcatt ctgatcgcag tcaaccagct
     3241 tgggttcggc agtatcgtgc cgatcctgcc gctgtatgcc gagtcgttcg gcgtctcgaa
     3301 ggcggccatc gggctgacga tcgccatcta cgggctggcg cgcttcctga tgaatgtgcc
     3361 gaccggctac gtctccgacc gctacgggcg gcgggtggcg ctggcgatcg gcggcgcgat
     3421 aaccgtcttc ggcaatttcg tctgcgcggt ggccggcagc tactggctct tcctcggcgg
     3481 gcgcttcgtg gccggcgccg gcgcggcgat ggtgattacc gggacgcaga tcatcgtcgc
     3541 cgatatctcg aagccggcca accggggccg cctgatgtcg atctacatgg gcaccttcat
     3601 gttcgcggtc ggcttcgggc cactgccggg cggcctgctc ggcgagcatg tctcgctggc
     3661 ggcgccgttc tgggtctact gcttcctcgg cgggatcttg acgatcctgg cctggacgaa
     3721 gatgccggag acgcgcggct ggcgcgagga tggcagcctt caggcgcagg cgccgagcct
     3781 gccactcggc cagcagttgc ggctgctgac ctcccagctg ggcttcacgc tgatcgcgat
     3841 cgtctccttc tgcaccttct tcgcgcgaac cggagggctg ttcaacctga ttccgacgct
     3901 ggccgaagac aagatcggcc tgacctccgg ccagatcggc gccgggctgg cgatgatcag
     3961 cctgatgggg ctgctgctgg cctatccgag cggcgtgatg gttgaccgct acggccgcaa
     4021 gtgggtgatc gtcctcagta ccgtcatcag cggcttctcg ctgatcgcgt ttgcccaggc
     4081 gccgaacttc ctctggttca tcgtcgcctg ctcgatgtgg gcaacctcct cgggcatcgc
     4141 cagcgccgcg ccgagcgcct acgcggccga catggccccg ccaggcatga acgccccggc
     4201 catgagcatg tacaggatgc tggccgactt cggctacgtg gccggcccgc tgatcctcgg
     4261 cagcgcggcc gatgtcttcg caaccgagac ggccctctac gcgacctccg cgatggtcat
     4321 cgtcgccggc ctggccttcg cctggctcgc cccggagagc ttccgccgcg aaccggccgc
     4381 gaccccggcc atcgccggcg gttctgggcg gggttgaccc aacagaacgt cgtctgcgtg
//
LOCUS       DASUGJ010000724         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1649285, whole genome shotgun sequence.
ACCESSION   DASUGJ010000724 DASUGJ010000000
VERSION     DASUGJ010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649285"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2088
                     /locus_tag="VFV93_16370"
     CDS             <1..2088
                     /locus_tag="VFV93_16370"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5166983.1"
                     /translation="IVTLALPAIASARSSIEGQGGRSRARTRGIWQRGAIDITLLAVA
                     GLAFYQLRRYGLPLTESVRGRLEVDPLLVAAPAIGLLAGALIALRLVPLLAAFAERLA
                     TRRGRAVTVLSAWQVARRPLRYARSALLLILALAIGLFTVSYSSTWRVSQGDQADFQI
                     GADVRVQPNQRPNAISTPYLAGAYRSIDGVSAVMPYWRSFRSVGRSRETADVVQIDAD
                     QAAEVVRIRDDLTTEPIDRLLAPLIEERPEIVTIPIPGEPRRLAIDLKASAAQGASIN
                     LSVVIRDADGAFQRLDAGSLEANEQPERVSVPLQGTLPDGSPASVAYPIELVAIEFVA
                     RTVPEPRTGTFELDGIATSASDEGDDWQPVDAQLESGAWQFDGDATRDGISQPPQIER
                     MSQPEIPDGSVGLRYNTGTSWQDFVNVPYRLRPAGSRVPNEIPALVSRHLLDELAFDI
                     GDTFTMDLVGGGQKLKIAGVLDAFPTLDAEASLFVIVDSETMSAYDLERPGTPFQAPA
                     EYWLAVDDDHEAAVAEALREIPFRSTAVAPQHERARILRTDPVALGMIGALSIGFVAA
                     IVFALIGFVTSAAVSVRERLTEFALLRAIGLSPRQLAGWLLLEHGFLLVVSLVFGTLL
                     GLLLSWLVLPQISVTQAATRAFPSVEIVIPWGTVTLLELAAIATLVAVSGLLALLLRR
                     IGLGTLLRIGEDA"
     gene            2085..>3233
                     /locus_tag="VFV93_16375"
     CDS             2085..>3233
                     /locus_tag="VFV93_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166984.1"
                     /translation="MIGRYASQIAILLRHLRAERALIATVVVVVCLTSLVMAAIPRLY
                     TAMLDDELTYRMEQAPDQARNIQVSIPYRFAQDPGEDRLREKGDDYQAGLPDSIQPII
                     SGNEYVIETPNLVALPGTEADPDFQRSLQLRILSNVNDRIQITSGRLPKPSGTIRLSE
                     WRNIPGARGEPERQIFEAVISDPAAAEMAMHINELTRIIVTAGNAQALVNIVGTFNVI
                     DPQADYWNGDTWLTEPVIFGFASDFNQILAGAVLISPDAYSTLFESYEGDFTYRWNYY
                     VDPDALTTGNYRTVIAEARQLQFTTGPTDAILTGGHNRITLSNDLPRLLNDFALQTNV
                     ALTVIALSAVGLLVTGLAALALLAALIADRRRATIALIRGRGASAAQLF"
BASE COUNT          517 a         1160 c         1032 g          524 t
ORIGIN      
        1 atcgtgacgc tggcgctgcc ggccatcgcc tcggcccgat cgagcatcga gggacagggc
       61 gggcgcagcc gggcgcgaac gcgtggcatc tggcagcggg gagcgatcga catcaccttg
      121 ctggccgtcg ccgggctggc cttctaccag ctgcgccgct acggcctgcc actgaccgag
      181 tcggttcgcg ggcgcctgga ggtcgatccg ctactggtcg ccgcgccggc catcggcctg
      241 ctggccggcg cgctgatcgc gttgcggctc gtgccgctgc tggcggcgtt cgcggaacga
      301 ttagcgaccc ggcggggacg ggccgtcact gtcctgagcg cctggcaggt cgcacggcgc
      361 ccgctgcgct atgcgcgctc ggcgctgctc ttgatcctgg cgctggcaat cgggctcttc
      421 accgtctcct actcgtcaac ctggcgcgtc tcgcagggcg atcaggccga tttccagatc
      481 ggtgccgatg tccgcgtcca gccgaaccag cgaccaaacg caatcagcac gccctatctg
      541 gccggagcct accgcagtat cgacggggtc agcgcggtca tgccctactg gcgctcgttc
      601 cgctcggttg gccgctcacg cgagacggcc gatgtcgtcc agatcgacgc cgaccaggcg
      661 gccgaggtcg tgcgcatccg cgacgatctg acgaccgagc caatcgaccg gctgctggcg
      721 ccgctgatcg aggagcggcc ggagatcgtg acgatcccaa tccccggcga gccgcgcagg
      781 ctggccatcg acctgaaggc gagcgccgcg cagggcgcca gcatcaacct ctcggtcgtg
      841 atccgcgatg cggacggagc gttccagcgg ctggacgccg gcagtctgga ggccaacgag
      901 caaccggaac gcgtcagcgt tccgttgcag ggcacgctac ccgacggcag tccggccagc
      961 gttgcctatc cgatcgaact ggtcgcgatc gagttcgtcg cccggacggt acccgaaccg
     1021 cgcaccggca ccttcgaact ggacggcatc gccaccagcg cgagcgacga aggcgacgac
     1081 tggcagccgg tcgatgcgca actcgaatcg ggcgcgtggc agttcgacgg tgacgccacc
     1141 cgcgacggga tctcacagcc gccacagatc gagcgcatga gccagccgga gatccccgac
     1201 ggctccgtcg gcttgcgcta caacaccggc acctcctggc aggatttcgt caatgtcccc
     1261 taccggctgc ggccggccgg ctcccgcgta ccgaacgaga tccccgccct cgtctcccgg
     1321 cacctgctcg acgagctggc cttcgacatc ggcgacacgt tcacgatgga cctcgtcggc
     1381 ggcggtcaga agctgaagat cgccggtgtc ctcgacgcct tcccgaccct cgacgccgag
     1441 gcgagcctgt tcgtcatcgt cgattccgag acgatgtctg cctacgatct ggagaggccc
     1501 ggaacgccat tccaggcgcc ggccgagtac tggctggccg tcgatgatga ccatgaagcg
     1561 gctgtcgccg aagcgctgcg cgagatcccg ttccgctcga cggcggtcgc gccgcagcac
     1621 gaacgcgcac gcatcctgcg gaccgacccg gtcgcgctgg ggatgatcgg cgcgctctcg
     1681 atcggcttcg tcgcggcaat cgtcttcgcg ctgatcggct tcgtcaccag cgcggccgtc
     1741 tcggtccgcg agcggctgac cgagttcgcg ctgctgcgtg caattggcct ctcgccccgc
     1801 cagctggccg gctggctcct gctggaacac ggcttcctgc tggtcgtctc gctcgtcttc
     1861 gggacgctgc tcggcctgct gctctcgtgg ctggttctgc cgcagatctc ggtgacacag
     1921 gcagccaccc gcgccttccc atcggtcgag atcgtcatcc cgtgggggac ggtcacgctg
     1981 ctggaactgg ccgctatcgc aacgctggtc gccgtcagcg ggttgctggc gctgctgctg
     2041 cgccggatcg gcctcgggac gttgctgcga attggcgagg acgcatgatc ggtcgctacg
     2101 ccagccagat cgccatcctg ctacgtcacc tgcgggccga gcgggcgctg atcgcgaccg
     2161 tcgtggtcgt cgtctgcctg accagcctcg tgatggcggc catcccgcgc ctctacacgg
     2221 ccatgctaga cgacgagctg acgtatcgga tggagcaggc gccagatcag gcacgcaaca
     2281 tccaggtcag catcccctac cgcttcgcac aggaccccgg cgaggaccgc ctgcgcgaga
     2341 aaggcgacga ctatcaggca ggcttgccgg actcgattca gccgatcatt agcggcaacg
     2401 aatatgtcat cgagacgccc aacctggtcg ccctgccggg aaccgaagct gatccggact
     2461 tccagcgctc gctgcagcta cgtatcctga gcaatgtcaa cgaccgcatc cagatcacca
     2521 gcgggcggct gccaaagcca agcggcacga ttcgcctctc ggagtggcgc aacatccccg
     2581 gcgcacgcgg cgaaccggaa cgacagatct tcgaggccgt catcagcgac ccggccgccg
     2641 ccgagatggc gatgcacatc aacgagctga cgcggatcat cgtcacggcc ggcaacgccc
     2701 aggcgctggt caacatcgtc ggcacattca acgtcatcga cccgcaggcc gactactgga
     2761 acggcgacac ctggctgacg gagccggtca tcttcggctt cgccagcgac ttcaatcaga
     2821 tcctcgctgg cgccgtcctg atctcacccg acgcatattc cacgctcttt gagtcgtacg
     2881 aaggcgactt cacctaccgt tggaactatt atgtcgatcc cgacgcgctg acgaccggca
     2941 actaccggac cgtcatcgcc gaagcgcgcc agctccagtt cacgacgggg ccgaccgacg
     3001 cgatcctgac cggcggccac aatcgcatca ccctcagcaa cgacctgccc cggttgctca
     3061 acgacttcgc gctccagacc aacgtcgccc taaccgttat cgccctctcg gcggtcggcc
     3121 tgctggtgac cggactggcc gcgctggcgc tgctggcggc gctgatcgcc gaccggcgac
     3181 gggcgacgat cgcgctgatt cgtgggcggg gcgcgtccgc ggcccaactc ttc
//
LOCUS       DASUGJ010000725        10446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1092014, whole genome shotgun sequence.
ACCESSION   DASUGJ010000725 DASUGJ010000000
VERSION     DASUGJ010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092014"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFV93_16380"
     CDS             <1..513
                     /locus_tag="VFV93_16380"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5166985.1"
                     /translation="RAGIGRRMAALMIDSVIVAIVVVPIGFLIQQSLENLTNEGVGWL
                     SASSVAMLLYIAYFTLLEGQSGATFGKRLLSLRVQRLHGGQCTMRESLIRNALRVIDG
                     FPFFFAPGVPPLYLAGGVFIMYSPHRQRLGDWAADTVVVLAHATEPVQRLATTASEPA
                     GVSGRKRRRR"
     gene            626..1354
                     /locus_tag="VFV93_16385"
     CDS             626..1354
                     /locus_tag="VFV93_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166986.1"
                     /translation="MQAVQDLATKILAASIEGNWGAVYDELHPAQQAVVNRDSFVQCR
                     SQITAPPYTVVVTQVGHDTMTAPEIYDRNSWAVLYNLQLEGYETISSRSHVYDEDGQL
                     SWYMDEPSMDAFRAGGCDVDPKLASSFALTIANAEIAGDWATAYDGLHPYQQAVVPRE
                     LFLECRSADHVQVTQVSESGVIMERFAAPEVPETESYAVTLRGLLADQTATYQTYHIF
                     DVGDRFGWVLGDLSIAAFQAGRCD"
     gene            complement(1472..1780)
                     /locus_tag="VFV93_16390"
     CDS             complement(1472..1780)
                     /locus_tag="VFV93_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5166987.1"
                     /translation="MRYRLVARPPSWMDVTLVLVEDAVGDLYLFNQLDRSLVQTFTDE
                     MQSLIHWFEFSQAVTWYTRSELIQKLSAVSIRIRLTRRRDSTGLQHALLAVACLRLLP
                     "
     gene            complement(1811..2953)
                     /locus_tag="VFV93_16395"
     CDS             complement(1811..2953)
                     /locus_tag="VFV93_16395"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5166988.1"
                     /translation="MPAFSDNDGNTTIRTARAMWLRYGAVLVAIGVVASLLTSSTGAQ
                     SGDPLRPQQWGHVQLHLDEAYDTSTGDGVVIAILDSGVDLDHPDLVDKLVPGWDFIEN
                     DDQPQDENGHGTHVAGIAAAATNNGIGIAGAAPDARIMPVRVMNALGVGDPEVISQAI
                     TWAADHGADIINLSLGGSTDLLSRIFKSGPMNAAIVDANSKGVLVVAAAGNDDTFIQA
                     YRDDVPVLVVNATNEAGLPAQFSNWGDPRAVAAPGTRILSTAPTEPTWIWKSGTDGYE
                     ELNGTSMAAPYVAGVAALLVATHRGDDPNTIRQILLDTSENPSGDPRLGAGIVNAEAA
                     VVRSDRNLWLIALAGTGIIIGIVALAWHYLLRPRRRARRTMVQSEA"
     gene            complement(3170..4135)
                     /locus_tag="VFV93_16400"
     CDS             complement(3170..4135)
                     /locus_tag="VFV93_16400"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5166989.1"
                     /translation="MKPLEAGAPRQDSASFERVVIPVDRSALSESAIPVGIMLAQELG
                     SSIDFLHVVDRNTGSKLNDAEAYLADITQRFPPPNDHRELVHVGEPEDAITNAAGDAE
                     AMVVMATHGYTGLRRMILGSVADAVIRQARVPIVLVRADREFRFPPEGFRRLLVPIDG
                     SERSARAAPLAAEIAQRSGGRLDLLHVVVPVAMSDLGSSMDPGYIPPEVYASMMDDLE
                     AVAREDLEAARRVCEQAGVDAALHRPIGTPVDSICRLAEEAGADAIVLSTHGRGGASR
                     VMMGSVATSVIHRCHIPVIIIPPQYGQSGTDETAVEAAGERCAEN"
     gene            4484..5182
                     /locus_tag="VFV93_16405"
     CDS             4484..5182
                     /locus_tag="VFV93_16405"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5166990.1"
                     /translation="MELRLNPVECSLADAICLSPLGGLPLVLRDRLLAGAIRVDVPAS
                     GMIYREDDVPRCALVVSGLVRVYMTSPTGREVTVRYGRRGDLLGIAALVGGPAPVNVQ
                     MLTDVTVLFLNTSTLAATARTDPAVAWLFTEDITQRLYETLGVLAHQTFGSLRQRIAG
                     HLLDLAAGHSNGCQLFAPVTQQAIADAVGSVRPAVARIVAQLRSQGLIETSPGGVIVL
                     DAAQLYEEVFPRVR"
     gene            5891..6376
                     /locus_tag="VFV93_16410"
     CDS             5891..6376
                     /locus_tag="VFV93_16410"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX5166991.1"
                     /translation="MSEWEAIVRLALAVALGGLIGLERETLKKDAGIRTHMLVTLGSA
                     GFMVASVLMVGVANPDGQVGRGDITRIGSTIVTGIGFIAGGLIFRSESQVQGLTTAAG
                     LWVTAAVGVLVGAGFYITAVGLTGLALFVLIVVRWGESRVEWTEGRSPRLRNPDTPDD
                     G"
     gene            complement(6652..7098)
                     /locus_tag="VFV93_16415"
     CDS             complement(6652..7098)
                     /locus_tag="VFV93_16415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5166992.1"
                     /translation="MSDDTGEAARMDRKHIFVVNGSPMFLDLMRMVLQDEHYNVTTTN
                     FVPRTFEQIIGLQPDLLIVDLAVKEMAGWTLLERLKQEAQTHEIPVIVVSTTPGHLEK
                     AEAESERYSGQLYVAKPFNLDDILDGVRSLIGGARRTEDNAVPASP"
     gene            7302..7820
                     /locus_tag="VFV93_16420"
     CDS             7302..7820
                     /locus_tag="VFV93_16420"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX5166993.1"
                     /translation="MADQALVIAWLNDAYSMEQSITQVLENHAKDAKDVPDAQARIEQ
                     HLEATRRHAELVKGLLDEFGETPSTTRSAMSTVMGKVQGMSTGLAKDELSDRGTRRPP
                     RARHPSRPDRSASDGLGSRLSRLGCADDRGRLVAHAARYAAGAGHADQAWSSIETRQS
                     ARTGVTRVGRLN"
     gene            complement(7922..8938)
                     /locus_tag="VFV93_16425"
     CDS             complement(7922..8938)
                     /locus_tag="VFV93_16425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5166994.1"
                     /translation="MVTATPTALPAYLQIEPVGQCNLRCQMCSIQFRQDGPPNGPLAF
                     MAWDTFTRLIDEFTGLEHLHLQGLGEPMMHPRFFEMVTYAAERGIRVTTNSNLTLLNE
                     RRAAKLVACGMDTLHVSIDGATAETYERIRVRSHYDKVVRNLGLLRAEQERTGSRSPH
                     IRMVMVIMRQNLHELPDLVRFAHEWRMEGLFVQHLCHDFGESSLPAHYRPMRDFVQEQ
                     TLFEEDLDLVEQYFAMAREVAADLDVDLRLPRTRMRIHNPGTPGRARCSWPWTGGYVS
                     YQGLAMPCCMVSTPDRINFGSMAQHGVPSIWNSAPFQTFRTQLDSDEPPGICRSCSIY
                     TGTF"
     gene            complement(8971..9948)
                     /locus_tag="VFV93_16430"
     CDS             complement(8971..9948)
                     /locus_tag="VFV93_16430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5166995.1"
                     /translation="MTLRVSVVVPTFNRPDLLDRCLNALVNQEMEPWSFEIVVADDAA
                     SEATCHRVEHWAGAACERGTFVCYVRTCGRRGPAAARNTGWRAARGEIIAFTDDDCVP
                     LPGWLAAGLSAFTGTVAAVDGRVVMPLPAEPTDYEKNAAGLEQAEFVTANCFYRRDVL
                     DAVGGFDEQFRAAWREDSDLFFRVLDLKQPVGHAPDAIVIHPIRPGRWGVSIGQQQKT
                     IYNALLYRKHPERYRERIQAAPPWRYYRIVGALATAFGAGLLGRRRTSLVAAGVWAVM
                     TGRFCVERIAETSRRPAHVAEMIVTSAIIPPLAVYWRIRGAIRYRVLFV"
     gene            complement(9945..>10446)
                     /locus_tag="VFV93_16435"
     CDS             complement(9945..>10446)
                     /locus_tag="VFV93_16435"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5166996.1"
                     /translation="IIGWYQHRIEFGPRALGARSILASPIHAEMQGRLNEIKDREDFR
                     PVAPVVLEEEAPHWFRGAASSPFMLFVYDVLPDKADQIPAVRHVDGTARIQTINRDQH
                     PLYYDLLKAFQARTGVPVLVNTSFNTRGEPIVCTPRDAIECFWTSPLDALVIGSFLLE
                     KPESPR"
BASE COUNT         1903 a         3151 c         3400 g         1992 t
ORIGIN      
        1 cgcgcgggaa ttggccggcg gatggccgcg ctcatgatcg acagcgtgat cgtcgcgatt
       61 gtggttgtcc cgatcggatt cctgatccag cagagcctgg agaacctcac gaacgagggc
      121 gttggctggc tgtccgcgag cagtgtcgcg atgctgctct acatcgcgta ctttacgctt
      181 cttgaaggcc aaagcggcgc tacgttcgga aaacgactgc tctcactgcg cgtacaacgc
      241 ctgcacggcg gtcaatgcac gatgcgggaa tcgctgatac gcaacgccct acgcgtcatt
      301 gacggttttc ccttcttctt tgctcccggt gtaccaccgc tctacctcgc cggtggagtc
      361 ttcatcatgt actcgccaca tcgccagcgg ctgggcgatt gggctgccga caccgttgtc
      421 gttctggcac acgcgactga gcctgttcag cgactggcga ccactgccag cgagccggcc
      481 ggcgtatcgg gacgcaagcg gcgacgacgg tgatcgtcag tcacgaggag gaattatgcg
      541 ctggttgttg atcggtgcca ttgtactgac ggtcgcgtgc ggaaatgagg gcgaagccgg
      601 agaatcggac tctacagctg acacgatgca ggccgtccag gatctcgcca cgaagatcct
      661 tgccgcgtcg atcgagggga actggggcgc ggtctacgac gagcttcacc cagcacagca
      721 ggccgttgtc aaccgtgact cgtttgtgca gtgccgatcg cagatcactg caccaccata
      781 cacggtcgtg gtgacgcagg ttggacacga cacgatgacc gcgcccgaga tctacgatcg
      841 caactcatgg gccgtgctct acaacctgca actggagggc tacgagacga tctcatcgcg
      901 gtcacatgtg tacgacgagg acgggcagct gtcgtggtac atggacgagc caagcatgga
      961 tgccttccgg gccggcggct gtgatgtcga tcccaagctg gcatccagct tcgcactgac
     1021 gatcgccaac gccgagattg ccggcgactg ggcgaccgcc tacgacggcc tgcatccgta
     1081 ccagcaggcg gtcgtcccgc gtgagctgtt cctcgaatgc cgttccgcgg atcatgtcca
     1141 ggtgactcag gtctccgagt cgggtgtcat catggagcgc tttgctgcac cggaggtgcc
     1201 ggagacggaa tcgtacgccg tgacgctgag gggcctgctg gccgaccaga cagcaaccta
     1261 tcagacgtac cacatctttg atgtcggcga ccggttcggt tgggtgctgg gcgatttgtc
     1321 gatcgcggca ttccaggcag gccgctgcga ttgataccac cgttccccac agtcgttcgc
     1381 cagcgtgaat atgacgccct gcccctacac gggacagggc gtctaccgct ctcgcgtcct
     1441 gcgtgaggac gcatgttcga tgcaaccgtc gtcacggcag caggcgcaag caagccaccg
     1501 cgagcaacgc gtgctgaagt ccggtactgt ctcgccgccg ggtgaggcga atgcggatgg
     1561 ataccgctga cagcttctgg atcagttcgg agcgggtgta ccaggtaacc gcttgcgaga
     1621 actcgaacca gtgtatcaac gattgcatct cgtcggtaaa cgtctggacg agcgatctgt
     1681 ctagctggtt gaacaggtag agatcgccaa cggcatcttc cacaaggacg agcgtcacat
     1741 ccatccagga gggcggacga gcgacgagtc ggtagcgcat gcgcccctct ctgtgacgtc
     1801 cgtgatcgcc tcaggcttcg gactgtacca tcgtccgtcg ggcccgacgt ctcggccgca
     1861 gcaagtagtg ccatgcgagt gcgacgatcc cgatgatgat ccctgttcca gccagtgcaa
     1921 tcaaccagag gttgcgatcg ctgcggacga cggcagcctc ggcgttcacg atgccggcgc
     1981 cgagtctcgg atcgccactt gggttctcac tggtgtcaag caggatctga cgaatcgtgt
     2041 tcgggtcgtc tccacgatgg gtagcgacga gcagcgcggc cacgccggca acatatggcg
     2101 ctgccatcga ggtgccattg agctcctcat agccatcggt gccggatttc cagatccacg
     2161 tcggttcggt tggagcggtc gagaggatcc gagttcccgg cgcggcgacc gcgcgcggat
     2221 cgccccagtt ggagaattgt gctggtagac cagcctcgtt ggttgcgttg acgacgagga
     2281 ctgggacatc gtcccggtac gcctgaatga acgtgtcgtc gttcccggca gcagcgacga
     2341 ccaggacgcc tttgctgttt gcgtcgacaa tcgccgcatt catcggacca ctcttgaaga
     2401 tccggctgag cagatcggtg ctgccgccaa gtgagagatt gatgatgtct gccccgtgat
     2461 cggcagccca ggtgatcgcc tgggagatga cttcaggatc accgacgccc aatgcgttca
     2521 tgacgcgtac cggcatgatg cgcgcgtccg gcgcggcacc ggcgataccg attccgttgt
     2581 tggtcgctgc cgcagcgatg cctgcgacgt gggtcccgtg gccgttctcg tcctgaggct
     2641 ggtcgtcgtt ctcgatgaaa tcccagcctg gaacgagctt gtcgaccagg tcaggatgat
     2701 cgaggtcaac gccactgtcg aggatcgcga tgacaacgcc atcgccggtt gaggtgtcgt
     2761 acgcctcgtc gaggtgcagt tgcacatgcc cccactgctg cggccgtagc gggtctccac
     2821 tctgagcgcc ggttgaggat gtcagcaacg aagccacgac gccgatcgcg accagtacag
     2881 cgccatagcg aagccacatt gcccgcgccg ttcgaatcgt cgtgttgccg tcgttatcgc
     2941 tgaacgctgg cacgtcgtta tgctcctctg cttgcggacc gcccggcgac gttaccgccg
     3001 gcaacggcgc atgcctgacc acatgtcatc gcgaacgtga ggacgcgatg acatgtgact
     3061 ggatgccatg ctgagagggt acgttgcgct ccggaggcag tgatcggtcg tccgggtgta
     3121 agtcaggggc gtattgcgcc gagcgcggat ggctggcagc ctctgaaatc tagttctcgg
     3181 cacaacgctc gccggcagct tcaactgctg tctcgtcggt cccggactga ccatactgtg
     3241 gcgggatgat gatgacgggg atgtggcagc gatggatcac gctggttgcg acacttccca
     3301 tcatcacgcg ggacgcgcct ccgcgtccgt gggtcgacag gacgatggca tcggcaccgg
     3361 cctcctccgc gagacgacag atgctgtcaa ccggagtgcc gattgggcgg tggagtgccg
     3421 catcaacgcc ggcctgctcg cagactcgcc tcgctgcctc cagatcctcg cgggccaccg
     3481 cctcgaggtc atccatcatc gacgcataga cctcgggcgg aatatagcct gggtccatcg
     3541 atgatccgag atccgacatg gcaaccggca cgacaacgtg cagcagatca agccggccac
     3601 ccgagcgctg ggcgatctcg gcggcgagtg gtgctgctcg ggccgatcgc tctgagccgt
     3661 cgatgggaac gagcagacgc cgaaatcctt ccgggggaaa tcggaactca cggtctgcac
     3721 gaaccagcac gatcggcaca cgcgcctgcc tgatgacggc gtcggcaaca ctaccgagaa
     3781 tcatccgccg caaaccggtg tagccatgtg tagccatcac gaccatcgcc tcagcgtctc
     3841 cggccgcgtt ggtgatcgca tcctccggct cgccgacgtg aaccagctcg cgatggtcgt
     3901 tcggaggagg gaaccgctgg gtgatatccg ccagatacgc ttcagcgtca ttgagtttgg
     3961 aaccggtgtt tcggtcaacg acgtgcagga aatcgatgct gctcccgagt tcctgcgcga
     4021 gcatgatccc taccggaatc gccgactctg acaacgccga tcgatcgacc gggatgacca
     4081 cgcgctcgaa agatgcgcta tcctgtcgcg gagcacctgc ttccaacggt ttcatcccgg
     4141 cctccgtatg cgaatgactg ttctgtccac cggtacccag aatagcgcca ggtgcggttg
     4201 acgggcatca accatcggac tacgaccttc tggccctccg tgacggtcct cgggcgaaaa
     4261 ggaggtaacc atggacgtga tgctgtgttg tcaagatggc cctttcgagg gctgggccgt
     4321 atgggctggt tgaagatggc cacctggaat gtcccgattg cggagaacca gggtatttca
     4381 tacgtgcatc gagtcggagg cgcggaagta ttttgcgctt gcgcggactt cgcttgagat
     4441 ttcggcaaac cgaaggcgct cggaatgcag ggtctgcaaa tcaatggagt tacgattgaa
     4501 tcctgttgag tgctcattgg ctgatgccat ttgtctgagt ccgctggggg gacttcccct
     4561 ggtactgcgg gatcgtctcc tggctggagc gattcgtgtt gatgtccccg caagcgggat
     4621 gatctaccgt gaggacgacg ttccgcgctg tgcgctggtc gtgtctggtc ttgtccgggt
     4681 atacatgaca tcaccgacgg gacgggaggt aaccgtccgc tacgggcgcc gtggcgatct
     4741 gcttggcatc gcagcgctcg tcggtggtcc ggctcctgtc aatgtccaga tgctgactga
     4801 tgtgaccgtc ctcttcctca acacctcgac gctcgccgcg acagcccgca ctgatccggc
     4861 cgttgcgtgg ctatttactg aagacatcac gcagcgactc tacgaaacgt tgggcgtgct
     4921 tgcccatcaa acgtttgggt cactccgtca gcgcattgcc gggcatctgc ttgacctcgc
     4981 tgccggccac tcgaatggtt gccagctgtt cgcgccggtc acgcagcagg cgattgccga
     5041 tgcggtcggc tcggtacgtc cggctgtcgc ccggattgtc gcgcaactgc gtagccaggg
     5101 attgatcgaa acgagtccgg gtggcgtcat cgtactcgac gcagcgcagt tgtatgaaga
     5161 ggtgttcccg cgcgttcggt agccctggca gcatcggtcg gtgacccagc gatccggccg
     5221 gatcgctggg cttcccccac cccgacgagt gcattggcat ggagaacaac gcactccgct
     5281 tggtagcaac gcggtatgga tcgagtcgat acggatccgc gtcgatcgta cccattacga
     5341 agtgtgctgg ctgtcacatt tgtgacatcg aggcgtgctc tctgtaacgg cccgccgcgc
     5401 agcgacatgc aaccattctg tagcaaggca cctgctccga gattctcagt cgaggtgagt
     5461 tcgctctcgt cgatcgatcg atggggcggt gccttgcctc ttccgctcct tgttcgcccc
     5521 gtcccgctcc gattcacgcc gtcgctgctc gcggctggtg gtgagataaa cgtcatcgaa
     5581 ccgtcgtctc ggccagtcga tgcgtcgtgg ctggacacac ctgcgaccca gctttggacc
     5641 atgcaggacg atgatggcgc ggtacgattc tcctttaatt gtcaccacat gccgcctggc
     5701 gcggcggctg aacgtgcgac aatgacctaa ccccttctcg agtgtgcggc tcggcagatg
     5761 ggaaggccgt cggtgcgtcg cacgatcgtg ctcccatccg cgaagaccgc gatggaagca
     5821 gacaacccac ccgagcgagg aacgctcaaa actggattgt gatcctggac gtcatcagaa
     5881 ggatctgagg atgagcgagt gggaagcgat cgtgcgtctg gcgttggcgg ttgcgttggg
     5941 cgggttgatt ggcctcgagc gagagacgct caagaaggat gccggtattc ggacccatat
     6001 gctggtgacg ctgggttcag cgggattcat ggtggcatcc gtgctgatgg ttggggtggc
     6061 caaccccgac ggccaggtcg gtcgcggtga tatcacgcgt atcggttcca cgatcgtgac
     6121 cggcattggc ttcatcgccg gcggccttat cttcaggagt gagtcgcagg tccagggcct
     6181 gacaacggcg gcagggctgt gggttacggc cgccgttgga gtactggttg gtgcaggctt
     6241 ctacatcacc gcagtcgggt tgacagggtt ggcgctcttc gtcttgatcg tcgtgcgttg
     6301 gggggaatcg cgagtcgagt ggacggaggg tcgttcgccc cgcttgcgta acccggacac
     6361 tccggacgat ggctgaagtc gccgctctca cccaagaggt gcacccttgc cggcggttga
     6421 acgtgaaagc tagcgggcag gcgacctgaa ccggcgatgt tctgcgacgc gaagccggct
     6481 agaactgcaa tgcccgcgcg aactcctgcg cggtgtcggc gtcatttcca cgcaaatgcg
     6541 ctcagcgacc gaccgcgccg cccggccaga caccatcagc taggcgagcg tgctcgccga
     6601 tgcactgagg acggagactc gtctccgtca tcgagtcgta ctggcggact gctacgggct
     6661 agcgggtacc gcgttgtcct cggtgcgccg tgcgccgcca attaacgaac gcacaccgtc
     6721 gaggatgtca tcaaggttga atggctttgc tacgtagagt tggccgctat agcgctcgga
     6781 ctcggcctcc gccttttcca ggtggcccgg tgtggttgag acgacgatga ccgggatctc
     6841 gtgggtctgc gcttcctgct tgagccgctc cagcagcgtc cagccggcca tctctttgac
     6901 cgcgagatcg acgatcagga ggtcgggttg caggccgatg atctgctcga aggtgcgcgg
     6961 gacgaagttc gtggtggtga cgttgtagtg ttcgtcttgc agaaccatgc gcatcaggtc
     7021 cagaaacatc ggcgacccgt tgacgacgaa gatgtgtttg cggtccatgc gcgccgcttc
     7081 cccggtgtca tctgacatcg accgaccccc gttctccagc gcgtggcccc aacggtcaga
     7141 gtatatctga gatcgtgtgc ctgctgattg tgtgtgcctg ctgatggagg gggcgaccgc
     7201 gattcggcag ttgcggactc gatggcacgt gacctgcatc gccgggcggc ctggggccca
     7261 acacgaccga tctctatgcc cttggggaga aggaggcgca catggccgat caggctctcg
     7321 tcatcgcctg gctgaacgac gcctatagca tggagcagtc gatcacgcag gtcttggaga
     7381 atcacgccaa ggatgccaag gacgtgccgg acgcgcaggc tcggatcgag cagcatctgg
     7441 aagcgaccag gcggcatgcc gaactggtca agggcttgct ggacgagttc ggtgagacgc
     7501 catccacgac caggagcgcc atgtcgacgg tgatgggcaa ggttcagggt atgagcaccg
     7561 gcctggcaaa ggacgaactg agtgatcgag ggactcgtcg accaccacgt gctcggcatc
     7621 catcacgtcc ggacaggagc gcatcagacg gtctgggatc tcggctttct cgcctggggt
     7681 gcgctgatga tcgcgggcgg ttggtcgctc acgcggcgag gtacgctgcc ggcgcaggcc
     7741 acgcggacca agcctggtcg agcatcgaaa cgcggcaatc ggcgcgaact ggtgtaaccc
     7801 gagtgggacg actgaactga ggcgccgcat gccagctgag tatcatgcca agcgcctgac
     7861 gacacacacg cctgagcgcg cctcagaatc gcctatcgcc acaggtagca ccaccgaaca
     7921 ttcagaatgt gccggtgtag atggagcagg agcgacagat cccgggtggc tcgtcggagt
     7981 cgagctgggt gcggaaggtc tggaacggag cgctattcca gatcgacggt actccgtgct
     8041 gtgccatcga gccgaagttg atccggtcag gggtggagac catgcagcac ggcatcgcca
     8101 gaccttgata gctgacgtac ccgcctgtcc atggccacga gcaacgcgct cgccccggcg
     8161 tgccggggtt gtggatgcgc atccgtgtcc gtggcaggcg gagatcaaca tccagatcgg
     8221 cagcgacctc acgcgccatc gcgaagtact gttcgaccag gtcgagatcc tcttcgaaga
     8281 gcgtctgctc ctggacgaag tcacgcatcg ggcgatagtg ggccggcagg ctcgactcgc
     8341 cgaagtcgtg gcagagatgc tggacgaaga gcccctccat ccgccactcg tgcgcgaacc
     8401 ggacgaggtc gggtagttcg tgcaggttct gccgcatgat gaccatcacc atccgaatgt
     8461 gtggcgagcg cgacccggtc cgctcttgct cggcccgcag taagcccaga ttccggacga
     8521 ccttgtcgta gtgggagcgg acccggatgc gctcgtaggt ctcggccgtc gcgccgtcga
     8581 tcgagacgtg gagcgtgtcc atcccacaag cgacgagctt cgccgcccgg cgctcgttga
     8641 gaagcgtcag gttggagttg gtcgtgacgc ggataccgcg ctcggcagcg tacgtcacca
     8701 tctcgaagaa gcgcgggtgc atcatcggct cgccgagccc ctgcagatgg aggtgctcca
     8761 ggccggtgaa ttcgtcgatc agccgagtga atgtgtccca ggccatgaag gcgagcgggc
     8821 cattcggcgg gccatcctgt cggaactgga tcgagcacat ctggcagcgc agattacatt
     8881 gcccgaccgg ctcgatctgc aggtacgccg gcaacgcggt cggtgtcgcg gtcaccatca
     8941 acggtctcca gtctgattcg atggccccgg ctagacgaac agcacccggt agcggatcgc
     9001 gccacgaatg cgccagtaga cagcgagtgg cggaatgatc gctgaggtga cgatcatctc
     9061 ggccacgtgt gcgggcctgc gggatgtctc agcgatccgc tcgacgcaga acctgccggt
     9121 catgacggcc cagacaccgg ccgcgaccag cgaggttcgc cggcggccaa gcagccccgc
     9181 gccgaatgcc gtcgccagcg cgccgacgat gcggtagtag cgccacggtg gcgcggcctg
     9241 aattcgctcg cggtagcgct ccgggtgctt gcgatagagc agcgcgttat agatcgtctt
     9301 ctgctgctgg ccgatgctga caccccagcg gcccggccgg atcgggtgga tgacaattgc
     9361 gtctggcgcg tgtccaaccg gttgtttcag atcgaggaca cggaagaaga gatcgctatc
     9421 ctcgcgccag gctgcccgga attgctcatc gaagccgcca accgcgtcaa ggacatcgcg
     9481 gcgataaaag cagttggcgg tcacgaactc ggcctgttca aggccggcgg cattcttctc
     9541 gtagtcagtc ggttcggcag gtagcggcat gacaacgcgg ccatcgacag cagcaaccgt
     9601 tccggtgaag gccgacaggc cggctgcgag ccagcccggt agcggcacac agtcgtcgtc
     9661 ggtgaaggcg atgatctcgc ctctggcggc acgccagccg gtgttgcggg cggcagcagg
     9721 tccgcgtctt ccgcaggttc ggacgtagca gacgaatgtg ccccgctcgc acgccgcgcc
     9781 cgcccaatgc tcgacccgat gacaggtcgc ctcactagcg gcgtcgtcgg ccacgacgat
     9841 ctcgaagctc cacggctcca tctcctggtt gaccagtgca ttcaggcagc ggtcgaggag
     9901 gtcagggcgg ttgaaggtcg ggacgacgac cgacactcgc agcgtcatcg tggagattcc
     9961 ggcttttcga ggaggaacga gccgatgaca agcgcgtcga gcggcgatgt ccagaagcac
    10021 tcgatcgcgt cgcgcggggt gcagacgatc ggctcgccac gggtgttgaa cgaggtgttg
    10081 acgaggaccg gtaccccggt acgcgcctga aacgccttca gcagatcgta gtagagcggg
    10141 tgctggtcgc gattgatggt ctggatgcgg gccgttccgt caacgtgacg gacggcgggg
    10201 atctgatcgg ccttgtcggg gaggacatcg tagacaaaga gcatgaaggg ggaggacgcc
    10261 gcgccacgaa accagtgcgg cgcttcctct tccaggacga ccggtgcgac cggtcggaag
    10321 tcctcgcgat ccttgatctc gttcaaccgc ccttgcatct cagcgtggat cggcgaggcc
    10381 aggattgagc gggcaccaag tgcccgcggg ccgaactcga tccggtgctg gtaccagccg
    10441 atgatg
//
LOCUS       DASUGJ010000726         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2064977, whole genome shotgun sequence.
ACCESSION   DASUGJ010000726 DASUGJ010000000
VERSION     DASUGJ010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064977"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFV93_16440"
     CDS             complement(<1..355)
                     /locus_tag="VFV93_16440"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX5166997.1"
                     /translation="MESKAKFLGHPLHQQLIVFPLGLLGMAVVFDLISRFADWPEIGR
                     AGWYMIAAGIISGLLAALFGLIDWLAIPGGTRAKRIGAIHGIGNVIIVVLFAIAWLLR
                     RDDPTAVETLPLVLEV"
     gene            complement(775..1992)
                     /locus_tag="VFV93_16445"
     CDS             complement(775..1992)
                     /locus_tag="VFV93_16445"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5166998.1"
                     /translation="MSQTIVIAGASVAGAQAAATLRSEGFAGRIVLAGAEPEPPYERP
                     PLSKGFLQGSVSEDEFALHETGWYAEQAIDMRLGTRATSLDITDRTITLDSGETFAYD
                     QLLIATGATPRRLAAPGGDLPGVHYLRTLADARLIRERLAAAQRVVVVGMGFIGAEIA
                     AVARQAGKDVVALEAGELPMLAALGKEAASRMAWVHRQHGVDLRLDDGVIAFEGSGRV
                     ERVVTASESTIESDLVIVGIGVTPATGWLDGSGVTIERGVLVDEFCRTNIPDIYAAGD
                     VARSWHPAYGEHLLVEHFDNAGNQGALAAKNMLGANEPYAPLPYFWSDQYDLSLQFAG
                     HNAGHDQVVTRGAVESGSWSAFYLREGRFVAALSVNRFKDFSAARRLLRAGKEVSAEQ
                     LADEAVELKGLLR"
     gene            complement(2011..2442)
                     /locus_tag="VFV93_16450"
     CDS             complement(2011..2442)
                     /locus_tag="VFV93_16450"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5166999.1"
                     /translation="MHPHITLITLGVQDLQRSVAFYRDGLGLPTQGIIGEEFEDGAVA
                     FFKLDGGLILALYPRHALNKDADLHTAAPAGIEPSVSFSLAHNVGSKEEVDDVMAQAT
                     AAGATIAKAAYDRVWGGYTGYFRDPDGHLWEIAWNPGGFGD"
BASE COUNT          437 a          872 c          848 g          435 t
ORIGIN      
        1 tgacttcaag gacgagtggt agcgtctcga ccgccgtcgg gtcgtcgcgg cgcagcagcc
       61 aggcgatcgc gaacaacacg acgatgatga cgttgccgat gccgtggatc gcgccgatcc
      121 gcttggcgcg tgtgccgccc ggtatcgcca gccagtcgat caggccaaac aacgcggcaa
      181 gcaggccgct gatgatgccg gccgcgatca tgtaccagcc ggcccgcccg atctccggcc
      241 agtcggcaaa gcgtgagatc agatcaaaga cgaccgccat ccccagcaac ccaagcggga
      301 agacgatcaa ctgctgatgc aatggatggc caaggaactt cgccttgctc tccatgggac
      361 aacctcctca ttgaggatga aaatggtggc ggccctgaca atggataagc aagggcggtg
      421 ccggggattt cgggattcgg ggcacgggat tcgggattcg ggacgcggga cgtgggacgc
      481 agaccagacg aacggcgttg gtgcggagcc gcgtcccttg gactgtcatc ctgagccgca
      541 gcgaaggatc gctcaacgtg agacgccggg gcgcgctaat cggtagagcg ctcttgaccc
      601 ttccgaacag ctcctgtcat caacgccagg cgatccttcg ctgcggctca ggatgacagt
      661 ctaaggggtt cgggttcctc accgacgccg ttcatctgaa acggtggctg cgtcccgcgt
      721 cccgaatccc gaatcccggc tcccgaatcc cgtgccccga atcccgaacc ccgatcagcg
      781 cagcaacccc ttcagctcca cagcctcatc cgcgagctgc tcggcgctga cttccttacc
      841 ggcgcgcagc aggcggcggg cagctgagaa gtccttgaag cggttgacgg agagggcggc
      901 gacgaagcgg ccctcgcgca ggtagaaggc ggaccacgag ccgctctcga cagccccacg
      961 cgtgacgacc tggtcgtggc cggcgttgtg gccggcgaac tggagcgaga ggtcgtactg
     1021 gtcggaccag aagtacggca gcggtgcgta cggctcgttc gccccgagca tgttcttcgc
     1081 cgccagcgcg ccctggttgc cggcgttgtc gaaatgctcg accagcaggt gctcgccgta
     1141 ggctgggtgc caggaacggg cgacatcgcc tgccgcgtag atgtcgggga tgttcgtccg
     1201 gcagaactcg tcgaccagca cgccgcgctc gattgtgacg ccggagccgt cgagccagcc
     1261 ggtggccggt gtgacgccaa ttccaacgat gacaaggtcg ctctcgatcg tcgactcgga
     1321 cgcggtcacc actcgctcga ctcgcccgct gccctcgaag gcgatgacac cgtcgtccag
     1381 ccgcagatcg acgccgtgct gccggtggac ccaggccatc cgcgatgcgg cctctttgcc
     1441 aagcgcggcc agcatcggca gctcccccgc ttcgagcgcg acgacatcct tccccgcctg
     1501 ccgggcgacg gccgcgatct cggccccgat gaagcccatg ccaaccacga cgacccgctg
     1561 ggccgctgcc agccgctccc gaatcagacg ggcatcggcc agcgtcctca gatagtgaac
     1621 gcccggcagg tccccaccag gggctgccaa ccgtcgtggt gtcgcgccgg tcgcgatcag
     1681 gagctggtcg taggcgaacg tctcgccgct gtccagcgtg atcgtccggt ctgttatgtc
     1741 gagagaagtg gctcgcgtgc cgagccgcat gtcaatcgcc tgctccgcgt accagcctgt
     1801 ctcatgcagc gcgaactcgt cctcgctgac gctgccttgc aggaagccct tcgagagtgg
     1861 tggtcgttcg tagggcggct ccggctcggc gccggccagg acgatccggc cagcgaagcc
     1921 ctcgctccgc agcgttgcgg cggcctgcgc tccggcgacg ctggcgccgg cgatgacgat
     1981 cgtctgactc atctgtactc gctctctcgg ttagtcgcca aagccgcccg ggttccaggc
     2041 gatctcccag aggtggccgt ccgggtcgcg gaagtagccg gtgtagccgc cccagacgcg
     2101 gtcgtaggcg gctttcgcga ttgtggctcc ggctgccgtc gcctgcgcca tcacgtcgtc
     2161 gacttcctct ttcgagccga cattgtgggc gaggctgaag ctgaccgacg gctcgatccc
     2221 ggccggtgcg gccgtgtgga ggtccgcgtc cttgttcagc gcgtggcgcg gatagagggc
     2281 caggatcagc ccaccgtcca gcttgaagaa ggcgacggcg ccgtcctcga actcctcgcc
     2341 gatgatgcct tgcgtcggca agcccagccc gtcgcggtag aaggccactg agcgttgcag
     2401 gtcctggacg ccgagcgtga tcaaggtgat gtgtggatgc atctggcgct taacctctct
     2461 gctgttccgt cacaatccgg cgcgcctcga ccggaatgat accgacgctc gaccggtgtc
     2521 cagcacgatc gtccgtgtta cgatgcccca caacgggacg acacaatcga gcgacgctgc
     2581 cggggatcgt tg
//
LOCUS       DASUGJ010000727         3080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_954107, whole genome shotgun sequence.
ACCESSION   DASUGJ010000727 DASUGJ010000000
VERSION     DASUGJ010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954107"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFV93_16455"
     CDS             complement(<1..480)
                     /locus_tag="VFV93_16455"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5167000.1"
                     /translation="MDATLLVAMLIVAVSGVIFGMTGFGFAMISVPPLLLLYGPETVV
                     ALTIGASMLTSVIVVLGNRQELDRRLVLTMLPGAFVGLLLGAWILEIVDPTVLKIGAG
                     AFVTAYSVLLLRGYQPTGMGSTRAATIAGTFSGALGTSTGLSGPPVVILFTARQLAVN
                     "
     gene            complement(551..847)
                     /locus_tag="VFV93_16460"
     CDS             complement(551..847)
                     /locus_tag="VFV93_16460"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX5167001.1"
                     /translation="MERLWFILPVALLTYLTRIAGFRLGTRQLPPTLDRFLVYVPVAA
                     FAALAIPGVAGGAGSLLARLIGAALLAVVIYRFSYLWAGLLAGMVGFWLVGMLG"
     gene            complement(847..1587)
                     /locus_tag="VFV93_16465"
     CDS             complement(847..1587)
                     /locus_tag="VFV93_16465"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX5167002.1"
                     /translation="MIARSRSASQRRTNRANPAFGEQLRLGFLAMIPLWPGVASFSVA
                     FAIMARAAGYSAVETQLMSMLVYAGSAQLAMVTLYAGGAGALSIVLTALILNLRHVLY
                     GLSVNGQVEPHERPRRSLLAFFLTDESYGIATREWLAGRGSAAFLLGTGFSLYAVFAL
                     MTLAGILFGSLLPDVERSGLDFIFPLSFLALLLPLLTSRLQILVAVVAGGIALTAGQV
                     FDGGVTILLATIAAASCGVLLERGREAG"
     gene            1765..>3080
                     /locus_tag="VFV93_16470"
     CDS             1765..>3080
                     /locus_tag="VFV93_16470"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5167003.1"
                     /translation="MAGMEATLDGLTIERDGRTAIYLQLAETLAWRIETGELLSGVRL
                     PSERDLAQQLGISRTTAVSAYRELESRGLVRGHVGRGTFVCAGPAESGAPFAWRGKVA
                     LGAQRTLDGSMRTLAKHGSNRAVISLATGAGALDLVPMDIFEELAGRVLRRGAHAALG
                     LGPTEGQPKLRRAIAERQGIPPEQVLIVNGAQQGLDLIARCLLDPGDTVIMDRPGYLG
                     AIQVFRAAGANIVGWDIARADPEELEDLLLRYRPKLLYTNPTFQNPTGRTLDLHERSD
                     LLELAVRYRLPVIEDDLYRDTWFSVPPPLSLQQLDRHQLVIQVSTYSKSIAGGLRLGW
                     LSAAEPIVDQLSLVKQRIDVASPSFEQLLIAEFLSGRRYDDHLAMMRTAHRERHDALI
                     AAIRRRLPPGTLNVAPVDGGLYLWCRISHEIDTRLLLDSATEAGVV"
BASE COUNT          569 a         1051 c         1014 g          446 t
ORIGIN      
        1 attgacggcc agctgccggg ccgtgaagag gatgacgacg ggcgggccgg agaggcccgt
       61 cgatgtcccc agcgcgccgc tgaacgttcc ggcgatcgtc gctgcccggg tgctccccat
      121 cccggtcggc tgatagccgc gcaacagcag gaccgagtag gccgtcacga acgctccggc
      181 gccgatcttg aggacggtcg gatcgacgat ctccagtatc caggcgccaa gcagtagccc
      241 gacgaaggcg cccggcagca tcgtcagtac cagccgcctg tccagctcct gccggttgcc
      301 gaggacgacg ataacgctgg tcagcatcga ggcgccgatc gtcagcgcca caactgtctc
      361 cgggccgtag agcagcagca gcggcgggac gctgatcatc gcgaagccaa agccggtcat
      421 cccgaagatg acgcccgaga ccgcaacaat cagcatcgcc accagcaacg tggcgtccac
      481 cagccgccca ttctgttccg aagcggatca ccgggggatg gtacatgacg ggcgggcgga
      541 gtgaccgtcg ctagccgagc atcccaacca gccagaagcc gaccatcccg gccagcagtc
      601 cggcccagag gtagctgaag cgatagatga cgaccgccag cagcgcggcg ccgatcaacc
      661 gggcgagcag cgacccggct cctcccgcga cgccagggat ggccagcgcc gcgaaggccg
      721 cgaccgggac gtagacgagg aagcggtcca gcgtgggcgg cagttgccgt gtgccgagcc
      781 ggaagcccgc gatccgcgtg aggtaggtga ggagcgcgac cgggaggatg aaccagagcc
      841 gctccatcaa ccggcctccc gcccgcgctc cagcagcacc ccgcagctgg ccgcggcgat
      901 cgtcgccagc aggatcgtca caccgccgtc gaacacctgc ccggctgtca gtgcgatgcc
      961 gcccgccacg accgcgacca ggatctggag ccgcgaggtc agcaacggca gcagcaacgc
     1021 caggaacgag agcgggaaga tgaagtcgag tccgctccgc tcgacatccg gcagcagcga
     1081 gccgaagaga atgccggcca gcgtcatgag tgcgaagacg gcgtagaggc tgaagccggt
     1141 tccgagcagg aaggcggcgc tgcctcgacc tgccagccac tcgcgggtcg cgatgccgta
     1201 cgactcgtcg gtcagaaaga aggccagtag cgagcggcgt ggcctttcgt gcggctcaac
     1261 ctggccgttg accgacaggc cgtagagaac atggcgcagg ttgagaatca gcgccgtcag
     1321 gacgatcgac agcgcgccgg cgccaccggc gtacagcgtt accatcgcca gttgggcgct
     1381 gccggcgtag acaagcatcg acatcaactg cgtctcgacg gcgctgtagc ctgccgcccg
     1441 cgccatgatt gcaaacgcga ccgagaacga cgccacaccg ggccagagcg gaatcatcgc
     1501 cagaaacccg agccggagct gctcaccgaa cgcggggttg gcgcgattcg tcctgcgctg
     1561 gctggccgac cgggatcgag cgatcatatg atcctaaccg tctatcgtcc gtataccggg
     1621 tagatgatcg cacatcaaac gtaaccagtc aaactggcta cagtggtgat gcgcttcgat
     1681 tgtcaggcca ttatctggac gggaccacga ttcgcgatat ccattcgccg agtggacctt
     1741 tcagtaatcc gccaagcgcc gataatggcc ggcatggagg cgacgctgga cggattgaca
     1801 atcgagcgcg atggccggac cgccatctac ctgcaactgg cggagacgct tgcctggcga
     1861 atcgagacgg gtgagttgtt gtcgggcgtg cggctgccct ccgaacgcga cctcgcgcag
     1921 cagttgggga tcagccggac gacagccgtc agcgcctacc gcgagctgga atcgcgcggg
     1981 ttggtacgcg gccacgtcgg gcgcggcacg ttcgtctgcg ctgggccggc cgagagtggc
     2041 gccccgtttg cctggcgcgg caaggtcgcg ctcggcgccc agcggacgct ggacggctcg
     2101 atgcggacat tggcgaagca cggctccaac cgggccgtga tatcgttggc gaccggcgcc
     2161 ggcgcgctcg acctcgtccc gatggacatc ttcgaggaac tggcaggacg cgtcctgcgt
     2221 cgtggcgcgc acgcggcgct gggactgggt ccgaccgagg gacagccgaa gctgcggcga
     2281 gccatcgccg agcggcaggg catcccgccg gaacaggtgc tgatcgtcaa tggcgcccag
     2341 caggggctgg acctgatcgc acgctgcctg ctcgatcccg gcgacaccgt catcatggac
     2401 cgccccggct atctgggcgc gattcaggtc ttccgagcgg ccggcgccaa catcgttggc
     2461 tgggatatcg cgcgggccga cccggaagag cttgaagacc tgctgctgcg ctaccggccg
     2521 aagctgctct acaccaaccc gaccttccag aacccgaccg gccggacgct cgatctccac
     2581 gagcgtagtg atctgcttga gctggccgtc cgttaccggc tgccggtcat cgaggacgat
     2641 ctctaccgcg acacctggtt cagcgtgccg ccaccgctct cactgcaaca gctcgaccgg
     2701 caccagctcg tcatccaggt cagcacctac tcgaagtcga tcgccggcgg gctgcggctg
     2761 ggctggctct cagccgccga gccgatcgtc gatcagctct cgttggtcaa gcagcgcatc
     2821 gatgtcgcct cacccagctt cgagcagttg ctgatcgccg agttcctgtc cggccgccgc
     2881 tacgatgacc acctggcgat gatgcgtacc gcccatcgcg agcgccacga tgcgctgatc
     2941 gccgccatcc gtcgtcgcct gccacccggc acgctcaacg tcgcgccggt cgatggcggt
     3001 ctctacctct ggtgccgcat cagccacgag atcgacaccc gtctgctgct cgacagcgcg
     3061 accgaagccg gcgttgtctt
//
LOCUS       DASUGJ010000728         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1507661, whole genome shotgun sequence.
ACCESSION   DASUGJ010000728 DASUGJ010000000
VERSION     DASUGJ010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507661"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..430
                     /locus_tag="VFV93_16475"
     CDS             107..430
                     /locus_tag="VFV93_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167004.1"
                     /translation="MSAIDKRVSDQDIESLARKLAEFTESLSPGERMAFEVIEQHLIT
                     QIDADEMDVSGFEFTGLDTMGADAHREELLRQARPSRAAQTGEGFWKTILSALGGSDD
                     RRRAR"
     gene            complement(657..1352)
                     /locus_tag="VFV93_16480"
     CDS             complement(657..1352)
                     /locus_tag="VFV93_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5167005.1"
                     /translation="MSQRPRPSAPLVVEPPEPSERTPLDPTSQDAFDRVIRGRRSVRR
                     FRDEPIEDVIVQRVLEAGLWAPSPHGTQPWRFAVITQRANRERLADAMAGSWRDNLEM
                     DNEPAEVIEGRLAGSRRRLLEAPVLVVISLYTSDSDRYPDPTRAEAERTMAIQSLGAC
                     VENMLLAAFASGIDAGWMCAPLFCPEIVAETLDLDRTLTPHGLIAMGHAAADPKRRSR
                     RSLDTLIVWDDRK"
     gene            complement(1386..1967)
                     /locus_tag="VFV93_16485"
     CDS             complement(1386..1967)
                     /locus_tag="VFV93_16485"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX5167006.1"
                     /translation="MSSGSDAPVIAFETAGEWQHWLEEHQSEQAGVWLKLARAGTGLA
                     SVSRGDALDVALCYGWIDGQARSLDEHYWLQRFTPRRPRSKWSQVNTRKVEELIAAGR
                     MQPSGLREIDRAKADGRWDAAYAPPSTATVPDDLQAALDANDEARAFFTTLDSRNRYA
                     ILHRIHDAKRPETRAKRIDDFVTMLADGRKIHP"
     gene            complement(1974..2972)
                     /locus_tag="VFV93_16490"
     CDS             complement(1974..2972)
                     /locus_tag="VFV93_16490"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5167007.1"
                     /translation="MRLKTIGVLLAVLLVLPVLAGCGEDEGSGDLDEVTIGLTFVPNI
                     QFAPFYVAIEKGYYEDAGLKVSLNHHAVGADQFGALVAGNEDMLMSAGDETMQARAQG
                     VDVVYVAEIFRKYPVGLIVPDNSDITDIHSLAGKKVGIPGEYGATYTGLLALLADAGM
                     TKDDIDIESVGFTQAQALLAGQVDAIMGYVNNEPIQLKKAGMETRTFPVSDAIPLVSN
                     GLVAMEEYLDDNGDQARAIIEATLKGVQFAIDNPDETVEIAKNFVPTLDDEQQAADAL
                     EVLNASIPLWRAEGAPLGSSSPEAWQQTADFLQSNGLLANPVPVDDVYRSEYLPED"
     gene            complement(3037..>3969)
                     /locus_tag="VFV93_16495"
     CDS             complement(3037..>3969)
                     /locus_tag="VFV93_16495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5167008.1"
                     /translation="GNSMTRRLVPRRRRLSQMLEGTRERMSASDAIESRPATKPVITP
                     AGAVVAGPRARRRRRESPIMRVLPPLVVGLLMLGFWQLLTATGAVREYFLPPPADVAR
                     AFQRGVESGDFWSYGRTTLQESLAGFALGVSIAVPLGYGIARSRLLARAVEPFVAASQ
                     ALPAVAIAPLLVLWLGYGLKAIAVLCALIVFFPMVVNTAVGIRTLDRDTLDAARVDGA
                     GFWPLLLHFELPLALPVILAGVRTALTLSITGAVVGEFVIGDRGLGGLLTIARGNFDT
                     PLAFATILMLMVLASIMYGIAWALERWAAHKFQE"
BASE COUNT          645 a         1288 c         1358 g          678 t
ORIGIN      
        1 tggttgtgtg tgatcctgat actgggaggc ggcggtagtg gccgccgaga gcgaccgtgc
       61 cgtggaacgt gactgcggca tcctcaacag gcgcgggagg cgcaggatga gcgcgattga
      121 caagcgggtt tccgatcagg acatcgaatc gctggcgcgt aagctggccg agttcaccga
      181 atcactctca ccgggcgagc ggatggcgtt cgaagtcatc gagcagcacc tgattaccca
      241 gatcgacgcc gacgagatgg atgtctccgg ctttgagttc accggtctcg atacgatggg
      301 cgccgacgcg caccgcgagg agttgctgcg ccaggcgcgt ccgtcgcggg ccgcccagac
      361 tggcgagggg ttctggaaga cgatcctctc ggcgctgggt ggtagcgacg atcggcggcg
      421 ggcgcgctag ccgctcaggt cagaaagacc ggcagttcgg caaaggccag aaccagcagg
      481 atgagcaacg cggcggcaat cgcgagactg cgttgtggat gctgtgcgat cgggtgcatg
      541 gaccagacct gtcattgatc gacataaggc attgaaccgc cgacggcggc gtgccgttga
      601 catatcacgt cctgtgccac atcgcattgt cgttcaccgc ccagagggca cccgctctac
      661 ttccggtcgt cccagacgat gagggtgtcc agcgagcgcc gggagcggcg cttcgggtcg
      721 gcggcggcat ggcccatcgc gatcagtccg tgcggggtca gcgttcggtc gaggtcgagc
      781 gtctcggcga cgatctcggg gcagaagagg ggcgcgcaca tccagccggc gtcgatcccg
      841 ctggcgaagg cggccagcag catgttctcg acgcaggcgc ccaggctctg gatggccatc
      901 gtccgttccg cttcggcgcg ggtcggatcg gggtagcggt cggaatcgct ggtgtagagg
      961 ctgatgacga ccaggaccgg cgcttcgagc aggcggcgac gcgagccggc cagccggccc
     1021 tcgatgacct cggccggttc attgtccatc tcaaggttgt cacgccagct gccggccatt
     1081 gcgtccgcca gccgctcgcg gttggcccgt tgggtgatga cggcgaagcg ccatggctgg
     1141 gtgccgtgtg gcgagggagc ccagaggccg gcctccagca cgcgctggac gatcacgtcc
     1201 tcgatcggct cgtcgcgaaa gcggcggacc gagcgccggc cacgaatgac gcgatcgaac
     1261 gcgtcctgcg aggtagggtc aagcggtgtg cgttccgacg gctccggtgg ctcgacgaca
     1321 agcggcgcgc tagggcgggg gcgttgggac atctgctcag gtctccggtt ccgtgaggga
     1381 tctcgtcatg ggtggatctt ccgcccgtca gccagcatcg tcacgaagtc gtcgatgcgt
     1441 ttggcgcgtg tctcgggccg tttggcgtcg tgaatgcggt gcaggatcgc gtagcggttg
     1501 cggctgtcga gcgtcgtgaa gaacgcccgc gcctcgtcat tggcgtccag cgccgcttgc
     1561 aggtcgtccg gcacggtcgc cgtgctgggc ggggcgtagg ctgcgtccca gcggccatcc
     1621 gccttggccc gatcgatctc ccgcagaccg gacggttgca tccggccggc ggcgatcagc
     1681 tcttcgacct tgcgggtatt gacctgcgac cacttgctgc gcggtcgccg gggtgtgaag
     1741 cgctggagcc agtagtgttc gtccagcgag cgggcctggc cgtcgatcca gccatagcag
     1801 agggcgacgt ccagcgcgtc gccccgcgag acgctggcga gccccgtgcc ggctctggcg
     1861 agcttgagcc agacgccggc ttgttcagac tggtgttcct caagccagtg ctgccactcg
     1921 ccggccgtct cgaaggcgat gaccggcgcg tccgatccgg aactcatgct gcgttagtcc
     1981 tccggcaggt attcggaacg gtagacatcg tcgacaggga ccgggttcgc cagcaggccg
     2041 ttcgattgca ggaagtcggc ggtctgctgc caggcttccg ggctgctgct gccgagcgga
     2101 gcgccctcgg ctcgccagag cgggatcgag gcgttgagca cttccagcgc gtcggccgcc
     2161 tgctgctcgt cgtccagcgt cgggacgaag ttcttggcaa tctcgaccgt ctcatcgggg
     2221 ttgtcgatcg cgaactggac ccctttcagc gtcgcctcaa tgattgcacg cgcctgatcg
     2281 ccgttgtcgt cgaggtactc ctccatcgcg accagcccgt tcgagacgag cggtatcgcg
     2341 tccgagacgg ggaaggtgcg cgtctccatc ccggccttct tgagctggat cggctcatta
     2401 ttgacgtagc ccatgatcgc gtcgacctgt ccggccagca gcgcctgggc ctgggtgaag
     2461 ccgaccgact cgatatcgat atcgtccttc gtcatcccgg catccgccag cagcgccagc
     2521 aggccggtgt aggtcgcgcc atactcgccg ggaatcccga ccttcttgcc ggccaggctg
     2581 tggatgtccg tgatgtcgga gttgtccggg acgatcaatc cgaccgggta cttgcggaag
     2641 atttcggcca cgtagacgac atcgacgccc tgcgcgcggg cctgcatcgt ctcgtcgccg
     2701 gccgacatca gcatgtcttc gttaccggcg accagtgcgc cgaactggtc cgctccaacg
     2761 gcgtgatggt tcagtgagac tttgagaccg gcgtcctcgt agtagccctt ctcgatcgcg
     2821 acgtagaacg gcgcgaactg gatattaggg acgaacgtca gcccgatcgt gacctcgtcc
     2881 agatcgccgc tgccctcatc ctccccgcag ccagccagca ccggcagcac cagcagcacc
     2941 gccagcagta ctccaatcgt cttcagccgc atcgtcttcc atgtcctctc agcactcgcc
     3001 aaacagctca atcacccgat ccgtgacgtg tggaactcat tcctggaact tgtgcgccgc
     3061 ccatcgctcc agcgcccagg cgatgccgta catgatggag gcaagcacca tcagcatcag
     3121 gatcgtcgcg aaggcgagtg gcgtgtcgaa gttgccgcgt gcgatcgtca gcagcccgcc
     3181 gaggccgcga tcgccgatca cgaattcgcc aacgacggcg ccggtgatcg acagcgtcag
     3241 cgccgttctg acgccggcca ggatgaccgg cagcgccagc ggcagctcga agtgcagcaa
     3301 cagcggccag aacccggctc cgtcgacacg ggcggcgtcg agcgtgtcgc ggtcgagcgt
     3361 ccggatgccg acggccgtgt tgacgaccat cgggaagaag acgatcagcg cgcagaggac
     3421 ggcgatcgct ttcagcccgt agccgagcca gaggaccagc agcggcgcga ttgcgacggc
     3481 cggcagtgcc tggctggcgg cgacgaacgg ctcaacggcc cgcgccagca accggctgcg
     3541 ggcgatcccg tagccgagcg ggacggcgat gctgacacct agcgcgaagc cggccaggct
     3601 ctcctgtagc gtcgtccggc cgtagctcca gaagtcaccg ctctcaaccc cgcgctggaa
     3661 ggctcgcgcg acatcggccg gtggtggcag aaagtactcc cggacggcgc cggtcgccgt
     3721 cagcagttgc cagaagccga gcatgagcag cccgacgacc agcggcggca ggacgcgcat
     3781 gatggggctc tcgcgccggc gacggcgtgc ccgtggtccg gcgacgaccg ccccggcagg
     3841 agtgataacc ggcttcgtgg ccggtctgga ttcgattgcg tcggacgccg acatacgttc
     3901 cctcgtgccc tccagcatct gcgacagtct ccgtcgtcga ggaaccagcc ggcgggtcat
     3961 gctattccc
//
LOCUS       DASUGJ010000729         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1649638, whole genome shotgun sequence.
ACCESSION   DASUGJ010000729 DASUGJ010000000
VERSION     DASUGJ010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649638"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFV93_16500"
     CDS             complement(<1..507)
                     /locus_tag="VFV93_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5167009.1"
                     /translation="MSETIRVLIVDDHSLVRRGMRAVLGTAPDIDVVGEAATGREAIA
                     AAANLAPDVIVMDLQMPDLSGIEATRAILAEQPDVKILVVTLFQDDNSVFLALRAGAR
                     GFVLKDADDDDLLGGIRAVAGGSAIFSPSVATRILTLFSDPRPAPVAVFPMLTDRERE
                     ILERIAQGY"
     gene            complement(500..2590)
                     /locus_tag="VFV93_16505"
     CDS             complement(500..2590)
                     /locus_tag="VFV93_16505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX5167010.1"
                     /translation="MHIAWIDRSAHSPSPITGAASRSWVLSLPWAICVVVIALLVPIA
                     ILDILNRHAEDVWIIADPYQVVAAVAYSAVGALITTRTRGNRLGWLFLLVGLVAALGS
                     FAAAWAVYSLVTRPGLPGGGVALWLNVVVGVIAFFPMFPLLLFPSGRIADRRTGAVTA
                     LTILTTVALVSALMTGEVVPPGFPALYERTPSPFSLPLTFIDPGLCVLVLGICGMLSL
                     ALLIGRFRAARGAIRKQYTWVVVAMVQLVVAFIADFLARASGSSAYMVTGPLLSLSFV
                     LIPVMMGIAILRHHIWDIDVFINRALVYLALSACVVATYIFIVGWLSTVFRSDSNLLF
                     SLAATGVVAVLFHPLRVYIQRLVSRMLFGQRDEPYAVIARLGQRLESTLAPDAVLPAI
                     VGTVREALKLPYAAIAVTGGGGPSYAVASGEPIADPVTLPLVYQHVPVGELHLAPRSP
                     GESFGMADLRLLDDLARQAGIAVHAVQLTHELQQARERLVETREEERRRLRRDLHDGL
                     GSQLAALNLQWGALPALIDSDPAAAKDEVAELRTQLRKAITSIRTLVHGLRPPAIDEL
                     GLLVAIEERLRQYDTGELVVESDLPARLPELPAAVEVAVYRIIEEALANISNHAQARR
                     CSVRMAIADDLLLEIEDDGVGIDAGYRAGVGLQSMRERAAELGGSFTIAPRPSGGTSL
                     SVRLPLPRETGPDE"
BASE COUNT          527 a          950 c         1038 g          410 t
ORIGIN      
        1 gtagccctgc gcgatccgct ccaggatctc gcgctcgcgg tcggtcagca tcgggaagac
       61 cgccaccggg gccgggcgtg ggtcggagaa gagcgtcagg atgcgggtcg cgaccgatgg
      121 gctgaagatg gccgacccac ccgcgacggc acgaatgccg ccgagcagat cgtcgtcgtc
      181 ggcgtccttg aggacgaagc cgcgcgcccc ggcccgcagc gccagaaaga cggagttgtc
      241 gtcctggaag agcgtcacga ccaggatctt cacgtcgggc tgctccgcga ggatcgcgcg
      301 ggttgcctcg atgccgctca ggtcgggcat ctggaggtcc atcacgatca cgtcgggagc
      361 gaggttcgcc gccgctgcga tcgcctcgcg gccggtcgct gcctcgccaa caacatcaat
      421 gtccggcgcc gtgccgagca cggcccgcat cccgcggcgg acgagcgagt gatcgtcgac
      481 gatcagcacc cgaatggtct cactcatccg gcccggtctc tcgcggcaat ggcagccgga
      541 cgctgagcga ggtgccgccc gagggacgcg gcgcgatcgt gaacgacccg ccgagttccg
      601 ccgcacgctc ccgcatcgac tgcaagccaa caccggcccg gtagccggcg tcgatgccta
      661 caccgtcgtc ctcgatctcc agcaggagat cgtccgcgat cgccatccgg acgctgcacc
      721 gccgcgcctg agcgtggtta ctgatgttgg ccagcgcttc ctcgatgatc cggtagacgg
      781 cgacttcgac agcggccggc agttccggca gacgggccgg aagatctgac tcgacgacga
      841 gctcgccggt gtcgtactgg cgcagccgct cctcgatcgc gaccagcagc ccgagttcgt
      901 cgatggcggg tggccggaga ccatgcacca gcgtccggat cgaggtgatc gccttgcgga
      961 gctgggtccg cagctcggcg acctcgtcct tggcggcggc ggggtcggag tcgatcaggg
     1021 ccggcagcgc gccccactgg aggttgagtg cggctagttg cgagccgagg ccgtcgtgca
     1081 ggtcgcgccg caatcgccgc cgttcttcct cgcgggtttc gaccagccgt tcgcgggcct
     1141 gctgcaactc gtgggttagc tggacggcgt gaacggcgat cccggcctgc cgcgccaggt
     1201 cgtcgagcag ccgcaggtcg gccatcccga acgactcgcc cggcgagcgt ggcgccaggt
     1261 gcaactcccc gaccggaacg tgctggtaga cgagtggcag cgtcaccgga tcggcaatcg
     1321 gctcgccgct ggcgacggca tacgaagggc caccgccacc ggtgacggcg atcgcggcgt
     1381 agggcagctt gagcgcctcg cgaacggtgc cgacgatggc gggcaggacg gcgtcgggcg
     1441 ccagcgtgct ctccagccgc tgccccagcc gggcgatgac ggcgtacggc tcgtcgcgct
     1501 ggccgaacag cattcggctg accagccgct ggatgtagac gcgcagcggg tggaagagga
     1561 cggcgacgac accggtcgcg gccagcgaga agagcaggtt gctgtccgag cggaagacgg
     1621 tgctgagcca gccgacgatg aagatgtagg tcgcgacgac gcaggccgac agcgccaggt
     1681 agaccagcgc gcggttgatg aagacgtcga tgtcccagat gtggtggcgc aggatcgcga
     1741 tccccatcat gaccgggatc agcacgaacg acagcgacag cagcgggccg gtgaccatgt
     1801 acgcggatga tccggacgcc cgcgcgagaa agtcggcgat gaaggcgacg acgagctgga
     1861 ccatcgcgac gacgacccag gtgtattgct tgcggatcgc accacgcgcc gcccgaaagc
     1921 gcccgatcag cagcgcgagc gagagcatgc cgcagatgcc gagtaccagc acacagagac
     1981 ccgggtcaat gaaggtcagc ggcaggctga atgggctggg tgtccgctcg tagagagccg
     2041 ggaagccagg cgggacgact tcgcccgtca ttagcgctga gaccagcgcg actgtcgtca
     2101 ggatcgtcag cgctgtcaca gcaccggtcc gccggtcggc gatgcggccg cttgggaaga
     2161 gcaagagcgg gaacatcggg aagaacgcga tcacgccaac gacgacgttc agccagagcg
     2221 ccaccccgcc gcccggcagc cccggccgcg tcaccagact gtaaacggcc caggccgcag
     2281 cgaaggaacc aagcgccgcg acaaggccaa ccaacaggaa gagccagccg agtcgattgc
     2341 cccgtgtgcg ggtcgtgatc agcgcgccga ccgcgctgta ggcaacagcc gcaacgacct
     2401 gatacgggtc ggcgattatc cagacatctt cagcatggcg gttgaggatg tccagaatcg
     2461 cgatcggcac cagcagcgcg atgacgacga cgcagatggc ccacggcaac gacaacaccc
     2521 acgaccggga cgccgcaccg gtgatggggg atggggaatg cgcgctacgg tcgatccagg
     2581 cgatgtgcat ggcgctgatc cagacgatcg cgatgacgag aaccggtgat gactgcctgc
     2641 gcgacctgtg ccgatcggtg cgcgctcagt gtatcggtcg ccgttcccgc tgggaagggg
     2701 gatccttccc gactggccgg gacatggttc cctgctgaac gtcccttcgt ggttgacacc
     2761 gtgaacctgc cgcgtagggg cgatggccgc agcccacctg ttccaactta atcggatgaa
     2821 cggcgttggc acttgcgttt ggcatagcgt ggggtcctga ggacgggtcg agcggcgatg
     2881 ctgacggcat acgccagcgg tggatcgggg gcggtgagcg tgatg
//
LOCUS       DASUGJ010000730         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1924965, whole genome shotgun sequence.
ACCESSION   DASUGJ010000730 DASUGJ010000000
VERSION     DASUGJ010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924965"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VFV93_16510"
     CDS             <1..842
                     /locus_tag="VFV93_16510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5167011.1"
                     /translation="PTLSKREAEDQTVVGPVVEVENVSLAFDDKVVLKDVSFTLMPGH
                     TKVILGASGSGKSTILKIILGLWKPDGGTVKVNGHRVDTMTEAELMTMRADVGMVFQE
                     GALFDSLTVGENVGYKLYEDSEMPQDEIDARIEEVLGFVGLREFLDRMPSELSGGQRR
                     RVAIARAMAAKPRILLYDEATTGLDPITAISVDEEMIKLRDLENVSSIIVTHQLRDAF
                     FVATHEAVRRQGNDGYEIVPAGAQKCDEAEFIMLKEGVISFEGNAAELRAATDPYLKS
                     FLS"
     gene            855..1895
                     /locus_tag="VFV93_16515"
     CDS             855..1895
                     /locus_tag="VFV93_16515"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5167012.1"
                     /translation="MPRTRSLAWAELKIGLMSLFALLMATLLIFLLSGSGGFFWQRYS
                     LKTMFSNIAGLKEGAPVRVAGVEVGSVSDLNFIGDQVEVVMEVNRDHQSRITSTSVAS
                     LGSVSLLGEAAVDITASSGGTPVPEWGYVKSGRATGTIGDVATQASTAIEQATALVND
                     LRAGRGTMGLLLTDEQLYRELTSLMVSVQAVTDGIAKGNGTLGRLMNDPAAAKSLEAS
                     LDNLQAVTARIRAGEGSIGKLLNDDALSKSLTSMTANFDTISGRINKGEGTAGKLITD
                     EQLYNRLNSLSDRIDKVMAGLQQGEGTAGQLLRDRQLYENMNGTMAELRSLVSDIRKD
                     PKKYLNVKVSLF"
     gene            1913..2179
                     /locus_tag="VFV93_16520"
     CDS             1913..2179
                     /locus_tag="VFV93_16520"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX5167013.1"
                     /translation="MSQQGNGGGMFVAFIAGAIAGAAVALLFAPATGEEAREYLGQRA
                     REGKDRASEAARQGREILNKQRENLTTAFDRARDQFQRETGQDA"
BASE COUNT          484 a          657 c          803 g          405 t
ORIGIN      
        1 cgccgacgct gtcgaaacgg gaggcggaag accagacggt cgtggggccg gtggtcgagg
       61 tcgagaacgt gtcgctcgcc ttcgacgaca aggtggtgtt gaaagacgtg agcttcacgc
      121 tcatgccagg gcacacgaag gtgatcctcg gcgcgagcgg ttcgggcaag tcgacgatcc
      181 tgaagatcat cctcggcctc tggaagcccg acggcggtac ggtaaaagtg aacggccatc
      241 gcgtcgacac gatgaccgaa gcggaactga tgacgatgcg cgccgacgtc gggatggtgt
      301 tccaggaagg cgcgctattc gactccctga cggtgggcga gaacgtcggc tacaagctgt
      361 acgaagactc cgagatgccg caggacgaga tcgacgcgcg catcgaagaa gtgctcggat
      421 tcgtcggatt gagggagttt ctcgatcgaa tgccatcgga gctctcgggt gggcagcggc
      481 gtcgcgtggc gattgcgcgt gccatggccg ccaaaccacg catcctgctg tacgacgagg
      541 cgacgaccgg gctcgacccg atcaccgcca tttcggtcga cgaggaaatg atcaagctgc
      601 gcgacctcga gaacgtgagc tcgatcatcg tcacgcatca gctgcgcgac gcatttttcg
      661 tcgcgacgca cgaggccgtg cggcgccagg gcaatgacgg atacgagatc gtgccggccg
      721 gcgcgcagaa gtgcgacgag gcggagttca tcatgctgaa agagggcgtg atctccttcg
      781 agggcaacgc ggccgaattg cgggccgcca ccgatccgta tctgaagagt tttctttcct
      841 aaaggtagga ctacatgcca cgcacgcgtt cactcgcctg ggcggagctg aagattgggt
      901 tgatgtcgct cttcgctctt ctgatggcga cgctcctgat cttcttgctc agcggatcgg
      961 gcggcttctt ctggcagcgc tactcgctga agacgatgtt ctcgaacatc gccggcctga
     1021 aagaaggcgc gcccgtgcgc gtggctggtg tggaggtggg ctcggtcagc gatctcaact
     1081 tcatcggcga tcaggttgaa gtggtgatgg aggtcaacag ggatcaccaa tcgcggatca
     1141 cgtccacgtc ggtggcgtcg ctcggatccg tgtcgctgct cggcgaggca gccgtcgaca
     1201 tcacggcgtc gagcggcgga acgccggtac ctgaatgggg ctacgtgaag tcaggccgtg
     1261 caacgggaac gatcggcgac gtcgcgacac aggcgagcac ggcgatcgaa caggctaccg
     1321 cgctcgtgaa cgaccttcgc gcagggcgcg gcacgatggg gctgctgctc accgacgaac
     1381 agttgtatcg cgaactgacc tcgctcatgg tttctgtcca ggcggtgacg gatgggatcg
     1441 ccaaggggaa tggcacgctt ggacgcctga tgaacgaccc ggctgcggcg aagtcgctcg
     1501 aggcgtcgct ggacaacctg caagccgtga ccgcccgcat ccgggcgggg gaaggcagca
     1561 tcggcaaatt gctcaacgac gacgcgctga gcaagtcgct gacatcgatg accgcgaact
     1621 tcgacaccat cagcggccgc atcaacaagg gcgaggggac agcggggaag ttgattacgg
     1681 acgagcagct ctacaacagg ctcaattcgt tgtccgatcg catcgacaag gtgatggccg
     1741 gcctgcagca aggcgaaggg accgcgggac aactgcttcg ggatcgacag ttgtatgaga
     1801 atatgaacgg gacgatggcg gagttgagaa gtctcgtctc cgacatccgc aaggatccga
     1861 agaagtatct gaacgttaaa gtgagcttgt tctgaaccga aaagagggcg tcatgtcgca
     1921 gcagggcaat ggtgggggca tgttcgtggc gttcattgcc ggcgcgattg ccggcgcagc
     1981 agtggcgttg ttgttcgctc cggccaccgg cgaagaggct cgcgaatacc tggggcagcg
     2041 cgcgcgtgaa gggaaggatc gcgccagtga agccgcccgc caggggcgcg agattctcaa
     2101 caaacagcgt gagaacctga cgaccgcatt cgatcgcgca cgcgatcaat tccagcgcga
     2161 gacagggcag gacgcgtgac accactgtcg gaaacgttcc tcgggttgat cgcgctcgcg
     2221 ttgctcgtca tggcaggcgg ccaggtggtc gtggtcatga tggccttgaa agcgacgcgc
     2281 ggtctgaacg acacgctcaa ccggatcgaa cgtgacctgc ggccgatcgt cagcaacgtg
     2341 caggcgatt
//
LOCUS       DASUGJ010000731         4901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1650023, whole genome shotgun sequence.
ACCESSION   DASUGJ010000731 DASUGJ010000000
VERSION     DASUGJ010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650023"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /gene="ruvB"
                     /locus_tag="VFV93_16525"
     CDS             complement(<1..960)
                     /gene="ruvB"
                     /locus_tag="VFV93_16525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5167014.1"
                     /translation="MTSRVVAGKHLDEDVAIETSLRPRRISEYIGQEKVKQTLSIFIE
                     AALARGEPLDHMLLYGPPGLGKTTLANIIGAEMSVGVRITSGPAIERAGDLVSILTNV
                     KAGDVLFIDEIHRLNRAVEEVLYPAMEDFAVDIILGKGPGARPMRLNLPKFTLVGATT
                     RLALLTSPLRDRFGGVFRLEFYDDESMRAIIMRSARILGVEIEEAGALEIARRSRGTP
                     RVANRLLKRVRDFAQVRADGVISDEVANSALSLLDVDELGLDDVDRRILLAIVEKFDG
                     GPVGIDTLAAATSEETDTIMDVYEPYLIQLGFLQRTPRGRVATR"
     gene            complement(1031..1198)
                     /locus_tag="VFV93_16530"
     CDS             complement(1031..1198)
                     /locus_tag="VFV93_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX5167015.1"
                     /translation="MSQSLPIRKELLDILACPVCKTAVRLEGDELVCDTCKRRYQVED
                     GIPIMLVEEVE"
     gene            complement(1173..2558)
                     /locus_tag="VFV93_16535"
     CDS             complement(1173..2558)
                     /locus_tag="VFV93_16535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5167016.1"
                     /translation="MTAVHVQFVTPLPPLPTGIAQYSRDLLRALDDRWPLDVVPEAGS
                     DPTILRSFRAGVPTVFQIGNSGFHRLAYKHALREPGVLVLHDVSLHHGRAGELLRSGG
                     SGYLKIMRERYGDDGARAVRDILAGRQVDDIGAFPLSEDYIERAKVTVVHSQYARRLV
                     QRFVPEADVVVVPMGVPLPALVEQAAARRALGLPASAFVVASITHVNPYKRIPVVLRA
                     MRRLIVHEPDAILVIAGSVAPGIDLKRQIALLNLDRNVRMLGYVSDDQARLVARAADV
                     CINLRYPSAGETSASLLRLLGAGRPVLVTDDEPMAEFPRDAVLPVPVDRFEDEMVSEL
                     LLLLARDETLREEAGAAARAFIAGEHSLAVMVDGYRAAIHRAFGIEMPSIALMTVDEE
                     APNLDVSETVPTTVSWLETRVVDGIAGLRLAGHDATIVVAARAMTQLGLNRLPAQQNG
                     RTDDEPVAPDS"
     gene            complement(2565..3593)
                     /locus_tag="VFV93_16540"
     CDS             complement(2565..3593)
                     /locus_tag="VFV93_16540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5167017.1"
                     /translation="MNILVLHTQVPFVRGGAEVLVDGLVKALRERDHTVDVVALPLAW
                     NPPEGLLTTALAWRMLDLARFNDRAVDRVICTKFPTWAVEHECKSLWLIHQHRQAYDL
                     YGTKLSEFTPDPASRETRDRVVDIDSLGIADCAPRFGISRNVCDRLRRYNGLDATPLY
                     PPVPRAGLRAEAYEPFILSAARLDDAKRVEPAIRAMAHVPRGLRLEIVGDGPNRHDLE
                     LLARSLGVDDRVTFRGRVSDDALQALYNTCSGVYYAPVDEDYGYSTVEALQASKPVVT
                     APDSGGVLEFVTDSETGIVTSLDGEALGAAFGVLADPVVARRLGEHGPALTANLTWDI
                     VVESLLDG"
     gene            complement(3590..3964)
                     /locus_tag="VFV93_16545"
     CDS             complement(3590..3964)
                     /locus_tag="VFV93_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167018.1"
                     /translation="MSAARDALQMQVERIRTALLERHGFAPGMELPRDIARSPLDDVQ
                     RLATVSAHWGIASDLPIVGRFLVLFRRVQRILLRWYINPIVDQQNAFNDAVVRALHEL
                     ETENEKLRTQLADRSRLDVPTQ"
     gene            complement(3961..>4901)
                     /locus_tag="VFV93_16550"
     CDS             complement(3961..>4901)
                     /locus_tag="VFV93_16550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5167019.1"
                     /translation="MPGLEAAIGRQPTPDVVMATNIGLEGLAITGLRVARRIGAAFVL
                     MPFIHLGRDDDAAARRYVSMPHQQALLRAADAVVAMTEIEARFLASVGVDRSRVVIAG
                     AGMTPVDVAGGDGARFRERHGLNGPLVAALGALAPDKGTRELVRAVARLNRAGRCVTL
                     ALAGPSLSTFERWYTALSDDQRRGTRLLGVISPEEKRDLLDAADIVALPSRTESFGIV
                     FVEAWANRKPVIAADAGAVPELVRDGENGLLVPFGDVPALSSAIDRLLDDKELARMLG
                     GRGYRLATERYTWAAVHERVKDAVEIALRVRKEGVR"
BASE COUNT          888 a         1724 c         1524 g          765 t
ORIGIN      
        1 gcgggtcgca acgcggccac ggggtgtccg ctgcagaaag ccgagctgga tcaggtacgg
       61 ctcgtagacg tccatgatcg tgtcggtttc ctcgctggtg gcagctgcca gcgtgtcgat
      121 cccgacaggg ccgccgtcaa acttctcgac gattgccagc agaatccgcc ggtcaacatc
      181 gtccagcccc aactcgtcaa catcgagcag cgagagcgcc gagttggcaa cctcgtcgga
      241 gatcaccccg tcagcccgaa cctgggcgaa gtcgcgcacc cgcttgagca gccggttggc
      301 gacgcgtggc gtgcctcgcg agcggcgcgc gatttcgagc gcgccagcct cttcgatttc
      361 gacgcccagg atccgagccg agcgcatgat gatggcccgc atcgactcgt cgtcgtagaa
      421 ctccagccgg aagacgccgc cgaaccggtc gcgcagcggc gacgtcagca gcgccagccg
      481 cgtcgtcgcg ccaaccagtg tgaacttggg caggttcagc cgcatcgggc gggcgcccgg
      541 gcccttgccg aggatgatgt cgactgcgaa gtcctccatc gccggataga gcacctcttc
      601 gacggcgcgg ttcaaacggt ggatctcgtc gatgaagagc acatcgccgg ccttgacgtt
      661 cgtcaggatc gagacgaggt cgcccgcccg ctcgatggct ggcccggatg taatccgcac
      721 gccaacgctc atctcagcgc cgatgatgtt cgccagtgtc gtcttgccga gccccggtgg
      781 cccgtacagc agcatgtgat cgagcggctc gccacgcgcg agcgccgcct caatgaagat
      841 cgagagcgtc tgcttgacct tctcctggcc gatgtactcg ctgatgcgac ggggccgcag
      901 gctggtctca attgctacgt cttcgtcaag atgcttgccg gctacgacgc gactcgtcat
      961 caaagggcca cctgtccgag ctggcgacta acaacgtgtc aagtatagca ccaatacacg
     1021 aacagatgtt ctactccacc tcctcgacca gcatgatcgg gatgccgtct tctacctggt
     1081 agcgccgctt gcaggtgtcg cagaccagct cgtcgccctc aaggcgaacg gccgtcttgc
     1141 agaccgggca agcgaggata tcaagcagtt ccttacgaat cgggagcgac tggctcatcg
     1201 tctgtccgtc cattctgctg cgccggcagc ctgttcaatc caagctgcgt catcgcccgc
     1261 gcagccacaa cgatcgtagc atcgtggccg gccaatcgca ggcctgcgat cccgtcaacc
     1321 acgcgcgtct caagccacga cacggtcgtt ggcaccgtct cgctcacatc gaggttcgga
     1381 gcctcctcat cgacagtcat caacgcgatc gagggcatct cgatgccgaa cgcgcggtgg
     1441 atggcagcgc gatacccgtc aaccatcacc gcaaggctgt gttcgcccgc gatgaaagcg
     1501 cgggcggccg ccccggcctc ttcccgaagc gtctcgtcgc gggcgagcag caacagcagc
     1561 tcagacacca tctcgtcttc aaaccgatcg acaggcaccg gcaggacggc atcgcgcggg
     1621 aactccgcca tcggctcatc atcggtcacc agcaccggcc gccctgcacc gagcagcctg
     1681 agcagactgg cggatgtctc tcccgcactt ggataacgca ggttgatgca gacatcagcc
     1741 gcccgtgcca ccagccgcgc ctggtcgtca ctgacgtagc ccagcatccg gacgttgcga
     1801 tcgaggttga ggagagcgat ctggcgcttc aggtcgatcc ctggcgcgac gcttccggcg
     1861 atcacgagga tggcatccgg ctcgtgaacg atcaaccgcc gcatcgcccg cagcacaacc
     1921 gggatgcgct tgtacgggtt gacatgcgtg atcgacgcga cgacgaacgc cgacgccggc
     1981 agcccgagcg ccctgcgcgc agccgcctgc tcgaccagcg ccggcaacgg cacgcccatc
     2041 gggacgacga cgacatccgc ttccggcacg aaacgctgga cgagacgacg cgcgtactgg
     2101 ctatggacga cggtcacctt cgcgcgctca atgtagtcct ccgagagcgg aaacgctccg
     2161 atgtcgtcaa cctgccggcc ggcgaggatg tcacggacgg cccgagcgcc gtcgtcaccg
     2221 taacgctcgc gcatgatctt caggtagccg gagccgccgg agcgcaacag ttctccggcc
     2281 cgaccgtggt gcagcgacac atcgtgcagg acgagcacgc caggctcgcg cagcgcgtgc
     2341 ttgtaggcca gccggtgaaa gccgctgttg ccgatctgaa agacagtcgg gacaccggcg
     2401 cggaacgagc gcaggatcgt cggatcggat ccggcctcgg gaaccacatc gagcggccag
     2461 cggtcgtcca gtgcgcgcag caggtcgcgg ctgtactggg cgatcccggt cggcagcggc
     2521 ggcaacggcg tgacaaactg cacgtgtaca gcggtcatca aacgtcaccc gtccagcaac
     2581 gattcgacga cgatatccca cgtcagattc gctgtcaggg ccggcccgtg ttcacccagt
     2641 cgccgcgcaa cgaccggatc agcgaggacg ccgaacgccg caccgagcgc ttctccatcg
     2701 agcgacgtca cgatccccgt ctcgctgtcg gtgacgaact ccaggacacc accactgtcg
     2761 ggagccgtca caaccggctt cgacgcctgc aacgcctcaa ccgtcgagta gccgtaatcc
     2821 tcgtcaaccg gcgcgtagta gacgccgctg caggtgttgt acagcgcctg cagggcatcg
     2881 tcgctcaccc gcccgcgaaa ggtgacgcga tcgtccactc ccagtgagcg ggccaggagt
     2941 tccaggtcgt gacggttcgg cccgtcgccg acgatctcca gccgcaggcc gcggggcaca
     3001 tgcgccatcg cccggatggc gggttcgacg cgcttggcgt cgtccagccg ggccgcgctg
     3061 aggataaacg gctcgtaggc ctcggcccgc agcccggcgc gtgggaccgg tgggtagagc
     3121 ggcgtcgcgt cgaggccgtt gtagcgacgg agccggtcgc aaacgttgcg gctgatgccg
     3181 aagcgtggcg cgcagtcggc gatgccgagc gagtcgatat ccaccacccg gtcccgcgtc
     3241 tcgcgcgagg cggggtcggg cgtaaactcg ctgagcttcg tcccatacag gtcgtatgcc
     3301 tgccggtgct ggtggatcag ccagagcgac ttgcactcgt gctcgacggc ccaggtcggg
     3361 aacttggtgc agatcacgcg atcgaccgcg cggtcgttga accgagccag atcgagcatc
     3421 cgccacgcga gcgccgtcgt cagcagcccc tccggcgggt tccaggccag cggcagcgcc
     3481 acgacatcga cggtatggtc ccgctcacgc agggccttca ccagcccatc gacaagcact
     3541 tccgcgccgc cgcgcacaaa cggcacctgc gtatgcaaga cgaggatgtt cattgagtcg
     3601 gcacgtccaa ccgcgaccgg tccgccagtt gcgtgcgaag cttctcgttc tccgtctcca
     3661 actcgtgcaa cgcacggacg acggcgtcgt tgaacgcatt ctgctgatcg acgatagggt
     3721 tgatatacca gcgcagtagg atgcgctgca ctcgtctgaa gagcaccagg aacctgccga
     3781 cgatcggcaa atccgaggcg atgccccaat gggcagagac ggtcgccagc cgctgaacat
     3841 catccagcgg cgaacgggcg atgtcgcgtg gcagctccat gcccggcgcg aaaccgtggc
     3901 gctccagcaa tgcagtccga atacgctcga cctgcatttg cagcgcatct cgcgcagcgc
     3961 tcatcgcacc ccctcctttc gcacccgcaa cgcgatctcg actgcgtcct tcacccgctc
     4021 gtgaaccgcc gcccaggtgt accgctcagt tgccagtcgg tagccgcggc cacccaacat
     4081 gcgggcaagt tccttgtcgt caagtagcct gtcgatcgcg gaagacagcg caggcacatc
     4141 gccgaacggc acgaggagcc cgttctcgcc atcgcggacc agttccggaa cggcgccggc
     4201 gtcggcagcg atgaccggct tccgattcgc ccaggcctcg acgaagacga tgccgaacga
     4261 ctccgtccgc gacggtagcg cgacgatgtc ggccgcgtcc agcagatcgc gcttctcctc
     4321 cgggctgatt acgcccagca gccgcgtccc gcgccgctga tcgtcactta gggcagtgta
     4381 ccaacgctca aaggtgctca acgacggccc cgccagcgcc agcgtgacac accggccagc
     4441 gcggttgagg cgcgcgacgg cccggaccag ttcgcgggtg cccttgtccg gcgccaacgc
     4501 gccaagcgca gcgacaagcg gcccgttgag cccatgccgc tccctgaatc gcgcgccatc
     4561 gccaccggcg acatctaccg gcgtcattcc ggctccggca atcaccactc gcgaccgatc
     4621 gacgccgacc gacgcgagga agcgcgcctc gatctccgtc atcgccacga cggcgtcggc
     4681 cgcccgcagc aacgcctgct ggtgcggcat tgacacgtac ctgcgggcag ccgcgtcgtc
     4741 gtcgcggccg agatggatga acggcatcag cacaaaggct gcgccgatcc gccgggcgac
     4801 ccgcaggccg gtgatcgcca gcccctccag cccgatgttg gtcgccatca ccacgtccgg
     4861 tgtcggttgc cgcccgatcg cagcctccag tccgggcatc c
//
LOCUS       DASUGJ010000732         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1788297, whole genome shotgun sequence.
ACCESSION   DASUGJ010000732 DASUGJ010000000
VERSION     DASUGJ010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788297"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFV93_16555"
     CDS             <1..367
                     /locus_tag="VFV93_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX5167020.1"
                     /translation="WSGPSSEPAPVQLDAAIIFAPTGALVPVALRATAKGGVVVCAGI
                     HMSDIPAFPYDILWGERQIRSVANLTRRDGEEFLRLAPQIPIHTDVLRYRLEDANRAL
                     DDLRHGRFHGAAVLDIGER"
     gene            complement(406..1257)
                     /locus_tag="VFV93_16560"
     CDS             complement(406..1257)
                     /locus_tag="VFV93_16560"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX5167021.1"
                     /translation="MNLASVTVGLKRQMNAVSGATRLPNTESSDGLIGIPSPRARRRG
                     SPAQRAPELHSGPTGTLIGPIVYGGLDGIVTTFAVVSGVAGADLGSGVILILGLANLF
                     ADGVSMAVGSYLSSKSEHEYYLRERRTERWEIEQFPEAERAELIGLYRERGYPDADAH
                     GLTEIQTRRPESWINAMMAEEHGLLDVEVNPLHRGLATFGAFVVAGSVPLLAFLFGLL
                     FPFVADIAFPLSAILSALALFGLGAAKVFVTERQLVRSGLEMLLVGGLAAGVAYLVGF
                     FLQGLGA"
     gene            complement(1428..2798)
                     /locus_tag="VFV93_16565"
     CDS             complement(1428..2798)
                     /locus_tag="VFV93_16565"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX5167022.1"
                     /translation="MSVRRTLRAPSRSTRSAPKRRWPGSRLVHSVDFQLIRASREARK
                     GVHGLGPGLITGVADDDPSGISTYTVTGATWGYQFLWTSVITLPMNIAVQSVCARVGL
                     VTGRGLAGTIRSEYGRRWLYPIVALLFVANTINIGADIVAIAAVIDMLTGLPATVLVL
                     PIGLGIALLEIIIPYPRFARYLKLLTLVIFAYVIGAFFASPDWGAAAAATVIPKVSLT
                     PDAIQTFVAILGTTISPYLFFWQVSEEVEEEESRGITPAAAPPGQMRSLLHAARLDVA
                     TGMVLANVGFYFIVLTSAATLHAAHQNDIQTAAQAAEALRPLAGSASSLLFALGIIGT
                     GLLAIPTLAGSSAYAASELFDWPEGLNKTFRRAPQFYIVIALATLLGGLMTFTGISEI
                     SALILAATVNGVIAPILLVFIMSIAGNRHVLGEYVFGRTTRIVGWATTVSMALAAILL
                     FATLRS"
     gene            complement(2801..3259)
                     /locus_tag="VFV93_16570"
     CDS             complement(2801..3259)
                     /locus_tag="VFV93_16570"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX5167023.1"
                     /translation="MNALVIYDSKFGNTRQIAEVIGSTLAADFNVSVQPVTDQPEVTD
                     IISLLVIGGPTHAHGISQPMRQFLDNLPRDAVQDMAIATFDTRFHKPKLLVGAASDGI
                     AKRLRRQGAHIVSEPESFWVESGEGPLSPGEAERARAWARTLVPSAVPVG"
     gene            complement(3314..>3569)
                     /locus_tag="VFV93_16575"
     CDS             complement(3314..>3569)
                     /locus_tag="VFV93_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167024.1"
                     /translation="YLAVLFVPPLQQFYEIEPLPLVAYPLIAAFVFAWLLSVRWIWRH
                     DVMAQLHARLAKAGQRINFQDRLRSRQVPTHRHADVPSRH"
BASE COUNT          737 a         1088 c         1111 g          633 t
ORIGIN      
        1 ctggtccggg ccgagcagcg aacccgcgcc ggtccagctc gacgcagcca ttatcttcgc
       61 gccgaccggc gcgctcgtcc ccgtcgcgct tcgggcaaca gccaagggtg gcgtcgttgt
      121 ctgtgccggc atccacatgt ccgatatccc ggcattcccg tatgacattc tgtggggcga
      181 gcgacagatc cggtcagtcg ccaatctgac gcgccgggac ggcgaagagt tcctgcggct
      241 tgcaccgcag attccgatcc atactgacgt tttgcgctac cggctggagg atgccaatcg
      301 ggccctggat gatctgcggc atggtcgatt ccacggtgcc gcggtccttg acatcggcga
      361 acgatgacag cacgaggaag cgcgctaatt catcaaggcg agcctctatg ctccaagacc
      421 ttgtaggaag aatccaacca ggtaggccac gccggcagcc agcccaccca ccaggagcat
      481 ctccagcccg ctgcggacca actgccgctc cgtgacaaac accttcgctg caccgagacc
      541 gaagagcgcc agcgcagaca ggattgcgga caggggaaag gcgatgtcgg cgacgaatgg
      601 gaacaagagt ccgaacagaa aggcgagtag cggcaccgac ccagctacaa cgaaggcacc
      661 gaaggttgcc agcccgcggt gcagtgggtt cacttcgacg tcaagcaacc cgtgctcctc
      721 ggccatcatg gcgttaatcc acgactccgg acgcctggtc tggatctcgg tcagcccatg
      781 cgcatcagca tctggatagc cgcgctcacg ataaagtccg atcagttcag ctcgttccgc
      841 ttcggggaat tgctcgatct cccagcgttc agtgcgccgt tcccgcaggt aatactcatg
      901 ttcgctcttt gacgacagat aggagccaac agccatcgag acgccatcag cgaacaaatt
      961 tgcaaggccg agaatcagga tcacaccgga accgagatcc gcaccggcga cgccgctcac
     1021 gacggcgaag gtggtaacga tgccgtccag cccgccatag acgatcggac cgatcaacgt
     1081 accagtcggc ccggagtgca gctctggcgc ccgctgtgcg ggcgaaccac ggcgacgtgc
     1141 gcgtggtgac gggataccga tcagcccgtc ggagctctca gtgttcggta atctggtagc
     1201 accactgaca gcgttcatct gtctcttcag tcctacggtc accgatgcca gattcaatcg
     1261 atcggcatgg cacgatcagt ctggacttcg tgccgttgca gtgttgtcga tgagatcctc
     1321 gacttcctga caatctcgtg gcgaaacctg tgaaccggtt ggtaaccagc catgaaccga
     1381 agcgtgcaac gatcgatcac aatgttgtcg aggggtgtct ccgtaattca ggatcggagc
     1441 gttgcgaata gcaggatggc agcgagtgcc atcgagacgg tcgtcgccca gcccacgata
     1501 cgggtcgttc ggccaaacac atactcgcca aggacatgac gattccccgc gatcgacata
     1561 atgaagacaa gcaggatcgg ggcaatgacg ccgttcacgg tcgcggccag gatgagcgcc
     1621 gagatctcgc tgatgccagt aaacgtcatc aagccgccga gcagcgtggc gagcgcgata
     1681 acgatgtaga actgtggcgc gcgccggaag gtcttgttca gcccctctgg ccagtcgaag
     1741 agttcggatg ccgcgtaggc ggatgatcca gccagcgtgg ggatcgccag caggccagtt
     1801 ccgatgatgc caagggcgaa tagtaacgag gatgcgctgc ccgcgagcgg tcgcaacgct
     1861 tcggctgcct gtgccgccgt ctggatatca ttctggtgcg cagcgtggag cgtggccgct
     1921 gaggtcaaca cgatgaagta gaacccgacg ttggcaagca ccatcccggt cgcgacatcc
     1981 agccgcgccg catgcagcag cgatcgcatc tggccgggtg gcgcggcggc tggagtgata
     2041 ccgcgcgatt cctcctcttc gacctcctcc gatacctgcc agaagaagag atacggcgag
     2101 atcgtcgtgc cgagaatcgc gacaaaggtc tgaatcgcat caggtgttaa cgagaccttc
     2161 gggatgaccg tcgcggcggc ggccgctccc caatccggac tggcgaagaa tgcgccgata
     2221 acataggcga agatgacgag ggtgagcagc ttgagatagc gtgcgaagcg tggatagggg
     2281 atgatgatct ccaacaacgc aatgcccagg ccgatcggca gcacgagaac ggttgccggc
     2341 aagccggtca acatgtcgat cacggcggcg atcgcgacga tgtctgctcc gatgttgatc
     2401 gtgttcgcga cgaacagcag cgcgacgatc ggatatagcc agcgacgacc gtattcagaa
     2461 cggatcgtcc cggccagtcc tctgccggtg acaaggccga cccgcgcgca gacgctctgg
     2521 acggcgatgt tcatcggcaa tgtgatgacg ctcgtccaaa gaaactggta gccccaggtt
     2581 gctccagtga cggtataggt tgagataccc gacggatcgt catccgcgac accggtgatg
     2641 aggccaggtc cgagtccgtg gacgcccttc cgggcctctc gcgaggcccg gatgagctgg
     2701 aagtcgacgc tgtggaccag acggcttccc ggccagcgac gcttcggcgc ggagcgtgtt
     2761 gaacgactgg gagcgcgaag cgtgcgtcgt accgacatcg tcagccgacc ggcacggcgg
     2821 acggtacgag cgtccgtgcc catgctcgtg cgcgttcggc ttcacctggc gacagcggtc
     2881 cctcaccgga ctcgacccag aagctttctg gttcgctcac aatgtgcgca ccctgcctcc
     2941 gaaggcgctt ggcgattccg tcggatgctg ccccgacgag cagcttcggc ttgtggaagc
     3001 gcgtgtcgaa ggttgcgatg gccatgtcct ggacggcgtc cctcgggagg ttgtccagaa
     3061 actgccgcat cggctggctg atgccgtggg catgcgttgg cccaccgatc accagcaggg
     3121 agatgatgtc cgttacctcc ggttggtcag tcacaggctg aacgctcaca ttgaagtcag
     3181 cagccagcgt gctgccgatc acctctgcaa tctgccgggt gttcccgaat ttcgaatcgt
     3241 agatcaccag cgcattcatg gttcccgtct cccttctgtg ggtcaccatc cagatgatcc
     3301 gcacaattgc ggatcaatgt cgcgacggta cgtccgcgtg gcggtgcgtc ggaacctgcc
     3361 gcgagcgaag ccgatcctgg aagttgattc gctgccctgc cttcgcgaga cgggcgtgga
     3421 gttgcgccat gacgtcatgt cgccagatcc atcgcaccga tagcagccag gcgaagacga
     3481 aggccgcgat caatggatac gccacgagcg gaagtggctc gatctcgtag aactgctgga
     3541 gcggaggtac aaacaggact gccaggtag
//
LOCUS       DASUGJ010000733         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_538433, whole genome shotgun sequence.
ACCESSION   DASUGJ010000733 DASUGJ010000000
VERSION     DASUGJ010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538433"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VFV93_16580"
     CDS             <1..1353
                     /locus_tag="VFV93_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5167025.1"
                     /translation="ALCLRTSVDLDLGDTAISAEFCSAEGEQTSFHLAWFPSHTGVPD
                     QPDAAAELSRTEGWWRDWSDRCTYQGPHREAVLRSLITLKSLTYAPTGGIVAAATTSL
                     PEEIGGVRNWDYRFCWLRDSAFTIAALARLGYTEEAVALGAWLRRAVAGDPAQMQIMY
                     GIGGEHRLTEFELPHLAGYEGSRPVRVGNAASDQVQLDIYGEVMTAVYRAVEAGVRPR
                     EDAAQSPGVNVEAVIDAVERRWRDPDEGIWEVRGQRQHFTYSKFAAWYAVDRALKLAE
                     ATGREAPVDRWQQLRDEIHEDICRNGYDAERNTFVQFYGARALDASLLLIPGSGFLPD
                     DDPRIAGTVDAIQRELASGPFVSRYSTDEAVDGLPGSEGAFLICSFWLVNALATVGRR
                     EEARRHLDALLALQNDVGLLSEEYDPSAKRMLGNFPQAFSHIGLLSSVLALHGGFEER
                     "
     gene            1453..>2340
                     /locus_tag="VFV93_16585"
     CDS             1453..>2340
                     /locus_tag="VFV93_16585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5167026.1"
                     /translation="MEESTSPAPATRNRGLAIRLSMAMFVLVVDTSLMNVSISAIVHD
                     LNTTVSGVQSAIAIEALVSAAFILIGSKIGDLWGRKRAYVLGLLGYAAGALAMMFAQG
                     LTTIIVFWALIGGLGASLLLPAMQSLVHGNFEGEERVRAYAQVGAAAAIATAVGPLLG
                     GFITTYLSWRVGFLLELILIAVVLSGIRQVRDVPYTGPRQIDVLGAALSVLGMGGIVL
                     GILVWQEGGESVAALLAVGAISLVGLVFWLLRRMRQSKPTLLDPRLFESNIFRLGISQ
                     QMLQQIALGGMIIALPIYLQ"
BASE COUNT          364 a          724 c          792 g          460 t
ORIGIN      
        1 gcgctctgcc ttcgcacatc cgtcgacctt gacctcggcg acaccgcgat ttcggctgag
       61 ttctgcagcg ccgagggaga gcagacgtcg tttcatctcg cctggttccc ctcccatacg
      121 ggcgtgcccg atcagccgga cgcggcggcg gaactctccc ggaccgaagg ctggtggcgg
      181 gactggtcgg atcggtgtac gtatcagggg cctcaccgcg aagccgtgtt gcgttccctt
      241 atcacgctga aatcgctcac ctatgcgccc accggtggca tcgttgccgc cgccacgacc
      301 tcgttacccg aggagatcgg cggcgtgcgg aactgggact atcggttttg ctggctgcgg
      361 gacagcgcgt tcacgatcgc ggcgctggcg cggctgggct atacggagga ggcggtcgcg
      421 ctgggcgcct ggcttcggcg agcggttgcc ggcgacccgg cgcagatgca gatcatgtac
      481 ggcatcggcg gcgaacaccg gctgacggag ttcgagctgc cgcacctggc cggctacgaa
      541 ggctcgcggc cggtccgggt cggcaacgcc gccagcgacc aggttcagct tgacatctac
      601 ggcgaagtga tgacggcggt gtatcgcgca gtagaggccg gcgtccgccc ccgggaggac
      661 gcggcacaat cgccgggcgt caacgtcgaa gcggtcattg acgcggtcga acgtcgttgg
      721 cgcgatcccg atgaggggat ctgggaggtc cgtggccagc gccagcattt cacctattcc
      781 aagttcgccg cctggtacgc cgtcgatcgg gcgctcaagc tcgcggaagc gaccggtcgg
      841 gaggcgcccg tcgatcgctg gcagcagctt cgggatgaga ttcacgaaga catctgccgg
      901 aatgggtacg acgccgagcg aaatacgttc gtccagttct atggcgccag ggcactggac
      961 gcgagcctgc tgctcatccc gggcagcggc ttccttcccg atgatgaccc gcgcatcgcc
     1021 ggcacggttg atgcaatcca gcgcgagctc gcatccgggc cattcgtatc gcgctactcg
     1081 acggacgagg ctgtggacgg cctccccgga agcgagggcg cgttcctcat ctgctcattc
     1141 tggctcgtca atgcgctggc aaccgtggga cgcagggagg aagcgcgccg ccatctcgac
     1201 gcgctgctcg cgctgcagaa cgacgtgggc cttctgagcg aggagtacga cccatcggcg
     1261 aagcggatgc tgggcaactt tccccaggct ttctctcaca tcgggcttct gtcttccgtc
     1321 ctcgcgcttc acggaggttt cgaagaacgc tagggccggt tcgttccacg gcgacagcag
     1381 ccgcgtctgc ctatctgtga acgggccggc tggacggcca tggcctcctc cttgtctcag
     1441 gagccgattg aaatggaaga gagcaccagc cctgcaccgg cgactcgcaa tcgagggctc
     1501 gcgatccggc tgtcgatggc gatgttcgtt cttgtcgtcg acacgtcgct gatgaacgtc
     1561 tccatttccg caatcgttca cgatctcaac acaacggtga gcggtgtcca gtccgcgatc
     1621 gcgatcgaag cgctcgtgtc tgctgccttt atcctgatcg gcagcaagat tggcgacctc
     1681 tggggacgca aacgggcgta tgtgttgggt ctgctcggct atgctgccgg cgcactcgcg
     1741 atgatgttcg cccagggcct gacgacgatc atcgtgttct gggcactgat cggtggcctg
     1801 ggcgcgtcgc tcctgctgcc ggccatgcaa tcgctcgtcc acggcaactt tgagggtgag
     1861 gagcgcgtga gagcctacgc ccaggtggga gcggcggccg caatcgccac cgccgtcggg
     1921 ccactgctgg gaggattcat caccacctat ctttcctggc gggtgggatt cctgctcgaa
     1981 ctgatcctga ttgccgtcgt gttgtcgggc atccggcagg ttcgggatgt gccgtacacg
     2041 gggccgcggc agatcgatgt gctcggtgcg gcgctctcag tgctgggtat gggcgggatc
     2101 gtgctgggca tcctggtgtg gcaggagggc ggggaatctg tcgctgcgct cctggcggtc
     2161 ggggcgatct cgctggtggg gctggtcttt tggctgttgc gtcgcatgcg tcaaagcaaa
     2221 ccgacactgc tcgatccgcg gctgtttgag tcgaacatct tcaggttggg catttcgcaa
     2281 cagatgctcc agcagatcgc tctgggcggc atgatcatcg cgctgccgat ctatttgcag
//
LOCUS       DASUGJ010000734         4316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1788421, whole genome shotgun sequence.
ACCESSION   DASUGJ010000734 DASUGJ010000000
VERSION     DASUGJ010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788421"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFV93_16590"
     CDS             <1..616
                     /locus_tag="VFV93_16590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5167027.1"
                     /translation="YLEGIESGVASTPEQQVRYLGVAREKADALELLISDLFDFSRLE
                     YLDQAPNLEPLDLTALLRKLVDGIRPRATAKDVTVAVEFEAEPCRVDADSHLLTRVIE
                     NLLDNALRFTPNGGTVRVTCEATPAGVAFSIADSGPGIAPADLPNLFAPLYRGESSRN
                     RRTGGAGLGLTIARRILLAHGGDLTAANRPEGGAIFTGTIPTAR"
     gene            696..1163
                     /locus_tag="VFV93_16595"
     CDS             696..1163
                     /locus_tag="VFV93_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167028.1"
                     /translation="MASLASLRSEQELIAPERSESQVLHELSTLLAGDAQASLRLVTS
                     DGSEIELPESVMRVLRRAADALANERAVAVEPFGRLISIVTAAQLLNLTPGAVEAVID
                     RHELPAVLTAGQQMLDVRDVIVWGAQHHERLLKGLDELTQMSQKMGLYDLDAS"
     gene            1163..1744
                     /locus_tag="VFV93_16600"
     CDS             1163..1744
                     /locus_tag="VFV93_16600"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5167029.1"
                     /translation="MSASGGVAALLDASVLVPAALRDTLLRVAGAGLYRVAFSELILD
                     EVRRALVANSLASVTSADRLIENIHAYFWFALVAGDEPPDLELSTDPGDRHVLAAAIE
                     AKVDIIVTANLRHVPNSELGPYGMTSMSPDQFLLMLAQRDANALLQIVTQQAAALKSP
                     PLSPADVLGRLRLHAPAFVEHLASLSDALRDDG"
     gene            complement(1749..3617)
                     /locus_tag="VFV93_16605"
     CDS             complement(1749..3617)
                     /locus_tag="VFV93_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX5167030.1"
                     /translation="MQHATTDPDEPAASSNGSAGLAAAQRHAGHELGDFTTTVRVVVI
                     ALLAIAIGAVSAVVALALLRLISFFTNVFFFGTFSFDDASPADNNLGVFIIIVPVIGA
                     LIIGLMARYGSEKIRGHGIPEAIEAILINGSRVEPRVAVLKPVSSAISIGSGGPFGAE
                     GPIIMTGGAVGSMLAQLLHLTSSERKTLLVAGAAGGMSATFAAPVAAVLLAVELLLFE
                     WKPRSLVPVALASATAAILRRYLIGEGPLFPTPPHAEFIGPEGFAGCILIGFLAAGLS
                     MLLTTGVYAAEDAFQLLPIHWMWWPAIGGLAIGVGGLIFPEALGVGYGVIGELLQGDA
                     TNRLIIGILVVKSLIWIIALGSGTSGGVLAPLLMMGGALGAAAANFLPNEGTGFWPLI
                     AMAAILGGTMRSPFTAAIFALELTRDTNSLLPLLIAVTIAHGVTSVALKRSILTEKIS
                     RRGFHVSREYAIDPLEILFVREVMRTNVAAVPFSTPVAEFDLSQDEINGHHAQRLYPI
                     VDDTKRLIGVVPRHALEVDHPDGLVVGDVMLPEPVVAYPDEILRVVVNRMAETGLTRF
                     PVVEGNPTGEVLGMISLEDLLKARVRNLEAERRRERVLPASFFLPRAVRRGARVPQ"
     gene            complement(3598..4074)
                     /locus_tag="VFV93_16610"
     CDS             complement(3598..4074)
                     /locus_tag="VFV93_16610"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX5167031.1"
                     /translation="MDQSPDITNADLQALAEFRYQIRHFLSFSERAARAAGIEPRQHQ
                     LLLALAGLPEGVQPTIRALADRMHLQHHSAVELVSRLEQRGMVVRDRDRHDRRQVLVR
                     LTPEGEAILRQLSTTHWAELRSRGPILARALNAIVTADNSFRPSPIPEDSLAARDN"
BASE COUNT          772 a         1352 c         1464 g          728 t
ORIGIN      
        1 ctacctggag gggatcgagt ccggcgtcgc cagcacaccc gagcagcagg tccgctacct
       61 cggtgtcgcc cgcgagaagg ccgacgcgct cgaactgctg atcagcgacc tgttcgactt
      121 cagccggctg gaatatctcg atcaggcgcc gaatctggag ccgctcgacc tgactgcgct
      181 gctacgcaag ctggtcgatg gcatccgacc ccgtgcgacc gccaaggatg tcacggtcgc
      241 ggtcgaattc gaggccgagc cgtgcagggt cgacgccgat agccacctgc tgacgcgcgt
      301 gatcgagaac ctgctcgata atgcactgcg cttcacgccg aacggtggga ctgtccgtgt
      361 cacctgtgag gcaacaccag ccggtgtcgc gttctcaatc gccgacagcg gccccggcat
      421 cgccccggcc gacctgccga acctgttcgc tccactctac cgcggcgaaa gctcgcgcaa
      481 ccgtcgcacc ggcggcgccg gcctcggcct gacgatcgcc cgccgcatcc tgctcgccca
      541 cggcggcgac ctcaccgccg ccaaccgccc cgaaggcggc gcgatcttca ccgggacgat
      601 cccgaccgcg cgataggtca tcggaggttg tgcgaggcgc tggaattcgc catcatgggg
      661 atcgtgttgt aggaacctgt tctggaggcg tcacaatggc atcgttggct tcgcttcggt
      721 ccgaacagga gctgatagcg cctgaacgga gtgaatctca ggtactccac gagctgagta
      781 cgctgctcgc tggtgatgct caggcgtcgt tgcggcttgt gacaagtgat ggctcggaga
      841 tcgagctccc cgaatcagtg atgcgggtgc tgcgacgtgc agcggatgcg ctggcgaatg
      901 agcgggcggt tgcggtcgaa ccatttggtc ggctgatttc gatcgtgacc gcggcacaac
      961 tgctcaatct gacaccggga gcagttgaag cagtgattga caggcacgag cttcctgctg
     1021 tgctcaccgc gggacagcaa atgctggatg tccgggacgt tattgtctgg ggtgctcagc
     1081 accatgagcg gctactcaag ggactcgacg agctcacgca aatgagtcag aagatgggcc
     1141 tgtacgatct cgacgcctct tgatgagcgc ttctggaggc gtcgctgcgc tgctcgatgc
     1201 atcagttctc gttccggcgg cgttgcgtga cacgttgtta cgcgtcgcgg gtgccggact
     1261 gtatcgcgtg gccttcagtg aattgatcct cgacgaggtc cggcgtgcgt tggtcgccaa
     1321 cagcttggca tctgtgacga gcgccgatcg cttgattgag aacatccatg catatttctg
     1381 gtttgcactc gttgctggcg atgaaccacc cgacctcgag ttgtcgaccg acccgggtga
     1441 tcggcacgtg ctggcggcag cgattgaggc aaaggtcgat atcatcgtca ccgccaacct
     1501 gaggcatgtt cccaacagcg agttgggacc ctacgggatg accagcatgt cgccggatca
     1561 attcctgctg atgctggcac agagggatgc caatgcgctg ctccagatcg tgacgcaaca
     1621 ggcagcggcg ctcaaatccc caccgctctc gcccgccgac gtgctggggc ggctgcgcct
     1681 tcacgcgccg gcgttcgtcg agcaccttgc atctctctcc gacgcgctgc gagacgacgg
     1741 ctgacccact actgcggcac tcgcgcgcca cggcggacgg cacggggcag gaagaagctg
     1801 gccggcagga cgcgctcgcg gcggcgttcg gcttcgaggt tgcggacgcg ggccttgagc
     1861 aggtcttcga gcgagatcat gccgagcacc tcgccggtcg ggttgccctc gacgaccggg
     1921 aagcgggtca gacccgtctc ggccatccga ttgacgacga cgcgcaggat ctcgtcgggg
     1981 taggcgacga ccggttccgg cagcatcacg tcgccgacga cgaggccgtc cggatggtcg
     2041 acctccaggg cgtggcgggg gacgacgccg atcaaccgct tcgtgtcgtc aacgatcggg
     2101 tagagtcgct gggcgtggtg gccgttgatc tcgtcctggc tgaggtcgaa ctcggcgacc
     2161 ggcgtcgaga atgggacggc ggcgacgttg gtgcgcatca cctcgcggac gaagaggatc
     2221 tccagcggat cgatcgcgta ctcgcggctg acgtggaagc cgcgccggct gatcttctcg
     2281 gtcaggatcg agcgcttgag ggcgaccgac gtgacgccgt gcgcgatcgt gacggcgatc
     2341 aggagcggta gcagcgagtt ggtgtcgcgg gtgagctcca gcgcgaagat ggcggccgtg
     2401 aatggtgagc gcatcgttcc gccgaggatg gcggccatcg cgatcagcgg ccagaagccg
     2461 gtgccctcgt tgggcaggaa attcgccgcc gccgccccga gcgcgccgcc catcatcaac
     2521 aatggtgcaa ggacaccgcc ggaggtaccg gagccgagcg cgatgatcca gatcagggat
     2581 ttgaccacca gaatgccgat gatcagccgg ttggtggcgt cgccctggag gagttcgccg
     2641 atcacgccgt agccgacgcc gagcgcttcc gggaagatca ggccgccaac gccgatcgcg
     2701 agaccgccga tggccggcca ccacatccaa tggatcggca acagctggaa cgcgtcttcg
     2761 gcggcgtaga cgccggtcgt cagcagcatc gagagtccgg ccgccaggaa cccgatcagg
     2821 atgcagccgg cgaacccttc cgggccaatg aattccgcat ggggcggagt cggaaagagc
     2881 ggtccttcgc cgatcaggta gcgacgcagg atcgcagccg ttgcgctggc cagtgcgacc
     2941 gggacgaggc tgcgcggctt ccactcgaac agcagcagtt cgaccgccag caggacggcc
     3001 gcaaccggcg cggcaaacgt cgccgacatg ccaccggccg cgccggcgac cagcagcgtc
     3061 ttacgctcgg aactggtcag gtgcagcagc tgcgccagca tcgagccgac ggcgccgccg
     3121 gtcatgatga tcggtccctc agcgccgaac gggccaccgg agccgattga gatggcggag
     3181 gagactggct tgaggacagc cacgcgcggc tcgacccggc tgccgttgat gagaatcgcc
     3241 tcgattgctt cggggatgcc gtggccgcgg atcttctccg agccgtagcg ggccatcagg
     3301 ccgatgatca gcgcgccgat gacggggacg atgatgatga agacgccgag gttgttgtcg
     3361 gccggcgagg cgtcatcgaa gctgaacgtg ccgaagaaga atacgttggt gaagaagctg
     3421 atcaaccgta gcagcgccag cgcgacgacg gcgctgaccg cgccgatcgc gatcgccagc
     3481 agggcaatga cgacgacccg aacggtcgtc gtgaagtcac ccaactcatg gccagcatgt
     3541 cgctgcgcgg cggccaggcc tgctgagccg ttggacgacg cggccggttc gtcggggtca
     3601 gttgtcgcgt gctgcaagtg aatcctccgg aataggtgac ggtcggaagc tgttgtcggc
     3661 ggtgacgatc gcgttgagcg cacgcgccag aatcgggccc cttgagcgca actcggccca
     3721 gtgtgttgtc gagagctggc gcaggatcgc ttccccctcg ggtgtcagcc ggaccagcac
     3781 ctgtcgccgg tcgtggcggt cacggtcgcg gacgaccatc ccgcgctgtt cgagccggct
     3841 gaccagctcg acggcgctgt ggtgttgcag gtgcatccgg tcggccagtg cccggatcgt
     3901 cggctgaacc ccctccggca accctgccag cgccagcagg agctgatgct ggcggggctc
     3961 gatgccggcc gcacgggcgg cccgctcgct gaatgacagg aaatgccgga tctgataccg
     4021 aaactcggcc agcgcctgca agtctgcgtt ggtaatgtca ggtgactggt ccaaacgtac
     4081 gccattccac gaatatatcg tactacgata caagtgtatc gtgccagtgt atcgggtgtc
     4141 aacgagcttg tgacgcgaga tgcgtgacgc gtaatgcgac ccaggtggac ggcgcccacg
     4201 aggcccgcac ccgccacccc tgacgctcct atgtcaaccg gagtcgtctg ccgggatgac
     4261 gaagggtgcc agatcattga gggttgggat gggacaatcc tgccaggcgc gccaga
//
LOCUS       DASUGJ010000735         4653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_399039, whole genome shotgun sequence.
ACCESSION   DASUGJ010000735 DASUGJ010000000
VERSION     DASUGJ010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399039"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..860
                     /locus_tag="VFV93_16615"
     CDS             108..860
                     /locus_tag="VFV93_16615"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX5167032.1"
                     /translation="MRVVRNVGYIKRRKRAAQIMTGLGLLMLLLSWAVYLLQPDIFLF
                     AMVGLALGFILFNGGMQQMSRWARRPRSDIVIDHELKSLNDRFTLIHYPDLPGRRPDH
                     VLIGPSGVLVMTTREAHGQISVKGTRWRSRRSPLLSLFSMGGPQLGNPSIENEEQVKT
                     VREFLTSQDVNVEPEGAVVFVADNVEVDIEDTPITILHATEIRHHLQETDAAVTVGGR
                     ERDQIAELLSRGEDLERSADKVTRPKKRVRAA"
     gene            857..1909
                     /locus_tag="VFV93_16620"
     CDS             857..1909
                     /locus_tag="VFV93_16620"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEX5167033.1"
                     /translation="MTMDADALARWNVAGHDAAVEGLATAVAAGRVSHAYLITGLEGV
                     GRTTLALALARALNCEAAPGQRPCNECDACRRIVRHVHPDIVLADMQWQETVVGKSRG
                     DQSRSRQRFSIDAIKWLREDIVTRPILGRWKMQIIDDAGKFSTDAPEAFLKTLEEPPP
                     YAVIVLIAESADQVAETIRSRCRHIALGAVPASAIRAELIRRGVEGATAERLSRGALG
                     RVAWAVRMAEDPRALEKWREQVETAFEHLTTPLGRITITGSLAREHTRKRDNTFELLD
                     IWTGLWRDALLLHSGLPDQVSFPHLADRLEPFAQRQDISGLYRALWATQRAGSDLDHN
                     VQARIALHAMVMQWPD"
     gene            1897..2949
                     /locus_tag="VFV93_16625"
     CDS             1897..2949
                     /locus_tag="VFV93_16625"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEX5167034.1"
                     /translation="MARLTLLPARSTAHEDHAAARERFRDRIRLLGAANAFRAGQIAC
                     GCDGCECALPAVEGGAVEIAQTDVAGVRFRDSGKTYYVDPSGRQLEIGQHVVVDSSRG
                     HELAQVVIAPQQVIASSLDGELRPVVRVASNEDLRLAESKRAEQGEALRTFAAKVRDH
                     KLPMKPVSAEYSFDGSRLTLNFSANGRVDFRELVRDLARHFGCRIELRQIGPRDEARL
                     LGGLGRCGRPLCCSTWLPQFADVSMTMAKTQDLSLNPDKVSGVCGKLLCCLSYENSQY
                     AEARTRLPRLGQDVTTEQGPGYVHALHILKEQVTIRLENGENVTLSPGEFTTLSTTSD
                     AAPRRGRRRVRRDSAG"
     gene            2957..4072
                     /locus_tag="VFV93_16630"
     CDS             2957..4072
                     /locus_tag="VFV93_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HEX5167035.1"
                     /translation="MPGWIVTADALESIAIGAGILGTGGGGNPYYGKLQARRLLDEGA
                     RIEVVSLDSIADDATVVSVGGMGAPTVSVERIPRGDESLIALRALEEHTGRRANYLIP
                     GEIGGSNSTRPMVVGAMAGLPVLDGDGMGRAFPELQMDTFSIYGVPIAPATLADPRHN
                     TVIFPTVSDAHTLERYARAVTIQMGGSAGYAFPIMSGAQAKATAVPGTMTLAREIGDA
                     VRAARRTHADPVAAVLSFGDGVLLFSGKIVDIERRMEGGFARGSVTIEGLADDRGERL
                     RIEFQNEYLVAERGETVLAVVPDLICIVDSETAEPVTTEILRYGLRVAVIGMPAPLKL
                     KTPEALAVIGPAAFGYDVPYDPLPGLYGGDVPGPPTG"
     gene            complement(4062..4319)
                     /locus_tag="VFV93_16635"
     CDS             complement(4062..4319)
                     /locus_tag="VFV93_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX5167036.1"
                     /translation="MTLVFDILSLFLRIMMWAIIIRAVLSWFDPRGQNPISRALIEFT
                     EPIVAPIRSIMPRMGMIDLSPMVAIFVIYMLQAMISNAVNP"
BASE COUNT          822 a         1592 c         1496 g          743 t
ORIGIN      
        1 ctgccatgct agactgcatg cagttcggac gaacgtcgtc ggaaccctga atcagcatgt
       61 cgatgacatc gccattcacc tctgccgaga ggagcagttg atccgccatg cgcgtcgtgc
      121 gaaacgttgg atacatcaag cgacgcaagc gtgccgcgca gatcatgacc ggcctcggcc
      181 tgctgatgct gctgctctcc tgggccgtct acctgctcca gccggatatc ttcctcttcg
      241 cgatggtcgg gctggcactc ggcttcatcc tcttcaatgg cggcatgcag cagatgagcc
      301 gctgggcccg ccgcccccgt agcgacatcg tcatcgacca cgaactgaag tcgctcaacg
      361 accgctttac attgatacat tatccggacc tgccgggtcg tcgtcccgac catgtcctga
      421 tcgggccatc cggtgtcctg gtgatgacga cgcgtgaagc gcacggccag atcagcgtca
      481 agggcactcg ctggcggtcg cgccggtcgc cgctgctctc gctcttttcg atgggcggcc
      541 cgcagctcgg caatccctcg atcgagaacg aggagcaggt caagaccgtt cgtgagtttt
      601 tgaccagcca ggacgtcaat gttgaaccgg agggcgcggt cgtcttcgtc gccgacaatg
      661 tcgaagtcga tattgaggac acgcccatca ccattctcca cgcgaccgag atccgtcatc
      721 atcttcaaga aacagacgcc gcggtaacgg tcggcgggcg cgagcgcgac cagatcgccg
      781 aactcctctc gcgcggcgag gatctcgagc gcagcgccga caaggtcacc cgtccgaaga
      841 aacgcgtgcg tgccgcatga cgatggacgc ggacgccctc gcacgctgga acgtcgccgg
      901 tcatgacgcg gccgtcgagg ggctggcgac cgctgtcgcg gccgggcgtg tctcgcacgc
      961 ctacctgatc accgggctgg aaggtgtcgg ccggacgacg ctggcgctgg cactggcccg
     1021 cgcactgaac tgcgaagccg cgcccgggca gcgtccctgt aacgagtgcg acgcctgccg
     1081 gcgcatcgta cgccacgtcc accccgacat cgtccttgcc gacatgcagt ggcaggagac
     1141 ggtcgtcggc aagtcgcgag gagatcagtc gcgctcacgg caacgcttct caatcgacgc
     1201 gatcaaatgg ctgcgtgagg acatcgtcac ccggccgatc ctcggccgct ggaagatgca
     1261 gatcattgac gacgcaggca agttctcgac cgatgcgcca gaggcgttcc tcaagaccct
     1321 cgaagagccg cccccgtatg ccgtgatcgt cctgatcgcc gagagcgccg accaggtcgc
     1381 cgagacgatc cgctcacgtt gccggcacat cgcgctcggg gcggtaccgg ccagcgcgat
     1441 tcgggccgaa ctgatccggc gcggcgtcga gggcgcgacc gccgagcgac tctcgcgcgg
     1501 cgcgctgggg cgcgttgcct gggccgtccg gatggctgaa gacccacggg cgctggagaa
     1561 gtggcgcgag caggtcgaga ccgccttcga gcatctgacg acgccgctgg gtcggatcac
     1621 gatcaccggc tcgctggcgc gcgagcacac ccgcaagcgc gacaacacgt tcgagctgct
     1681 cgatatctgg accggcctct ggcgtgatgc gctgctgctg cacagcggac tgccggacca
     1741 ggtctccttt ccgcacctcg ctgaccggct ggagccgttc gcccagcggc aggacatctc
     1801 cggcctctat cgcgcgctct gggcgaccca gcgggcaggc agcgatctcg accacaacgt
     1861 ccaggcccgg atcgcgctgc atgcgatggt gatgcaatgg ccagactgac gctgctgccg
     1921 gccagatcga cggcccacga agaccacgcc gccgcccgcg agcggttccg cgaccgcatc
     1981 cggctgctgg gcgctgccaa cgccttccgt gccggccaga tcgcttgcgg ctgcgatggt
     2041 tgcgagtgtg cgctgccggc cgtcgaaggt ggcgcggtcg agatagcgca gacggatgtc
     2101 gctggtgtcc gcttccgcga ctccggcaag acctactacg tcgatccatc cggccggcaa
     2161 ctggagattg gccagcatgt cgtcgtcgat tcgtcgcgcg gccacgaact ggcccaggtc
     2221 gtcatcgccc cccagcaggt gatcgccagc tcgctcgacg gcgagctacg gccggtcgtg
     2281 cgcgtcgcca gcaacgaaga cctgcgactg gcggagtcaa agcgggccga gcagggcgaa
     2341 gcgctacgga ccttcgccgc caaggtccgc gatcacaagc tgccgatgaa gcccgtctcc
     2401 gccgaatact cgttcgacgg ctcgcgcctc acactgaact tctcggccaa cgggcgggtc
     2461 gatttccgcg aactcgtccg tgacctggcc cggcacttcg gctgccggat cgagctgcgt
     2521 cagatcggcc cgcgcgacga ggcccgcctg cttggtggcc tgggccgctg tggccgcccg
     2581 ctctgctgct cgacctggct gccgcagttc gccgacgtct cgatgacgat ggccaagacc
     2641 caggatctgt cgctcaaccc cgacaaggtc tccggcgtct gcggcaagct gctctgctgc
     2701 ctctcctacg agaacagcca gtacgccgaa gcccgcacgc gcctcccccg cctcggccag
     2761 gatgtcacga ccgagcaggg accgggctac gtgcatgccc tgcatatcct caaggagcag
     2821 gtcacgatcc ggctcgaaaa cggcgagaac gtcaccctct cgccggggga gttcaccacc
     2881 ctcagcacga ccagcgacgc cgccccacgc cgtggccgcc gtcgcgtccg ccgcgattcg
     2941 gccgggtgag gtcgacgtgc ccggctggat tgtcactgcc gacgccctgg agagcatcgc
     3001 gatcggcgcc ggtatcctcg gcaccggcgg gggcggcaat ccctactacg gcaagctgca
     3061 agcgcgccgg ttgctcgatg aaggcgcccg aatcgaggtc gtcagcctcg actcgatcgc
     3121 cgatgacgca acggtcgtgt cagtcggcgg aatgggcgcg ccaaccgtca gcgtcgagcg
     3181 gattccacgc ggcgacgagt cgctaattgc gctgcgggcg ctggaagagc ataccggccg
     3241 gcgcgccaac tacctgattc ccggcgagat cggcggttcg aactcgaccc ggccgatggt
     3301 cgtcggcgcg atggccggcc tgccagtcct tgacggcgac gggatggggc gcgcgtttcc
     3361 ggaactccag atggacacct tctcgatcta cggcgtccca atcgcgccag ccacgctggc
     3421 cgacccgcgc cacaacacgg tcatcttccc gaccgttagc gacgcccaca cgctcgaacg
     3481 ctacgcgcgg gccgtcacga tccagatggg cggctcggca ggctacgcgt tcccgatcat
     3541 gtccggcgca caggcgaagg cgaccgccgt gcccggcacg atgacgctgg cacgggagat
     3601 cggcgacgcc gtccgtgcag cgcgccgaac gcacgccgac ccggtcgccg ccgtcctgtc
     3661 gttcggcgat ggcgtgctgc tgttcagcgg caaaatcgtc gacatcgagc gccggatgga
     3721 gggtggcttc gcgcgcggct cggtcacgat cgaagggttg gcagacgacc gtggcgaacg
     3781 gctgcggatc gagtttcaga acgagtacct ggtcgctgag cgcggcgaaa ccgtgctggc
     3841 cgtcgtgccc gacctgatct gcatcgttga cagcgagact gccgagccgg tcacgaccga
     3901 gatcctgcgc tacggcctcc gcgtcgccgt catcggcatg ccggcgccgc tcaagctgaa
     3961 gacgcccgag gcgctggccg tcatcgggcc ggcagccttc ggctacgacg tcccgtacga
     4021 cccattacca ggcttgtacg gcggcgacgt tcccggtccg cctacgggtt gacggcgtta
     4081 gagatcatcg cctgcagcat gtagatcacg aagatcgcga ccatcggcga caggtcgatc
     4141 atccccattc gcggcatgat cgagcggatc ggtgcgacga tcggctcggt gaactcgatc
     4201 aacgcccgtg agatcggatt ctgcccgcgt ggatcgaacc acgacaggac agcgcgaata
     4261 atgatcgccc acatcatgat acgcaggaac aggctgagaa tatcgaagac tagcgtcacg
     4321 ttttcacctt gataacgccg gatatcgacg agaggaccgg ctgaaaaccg gcaggcacca
     4381 cgacccacca ggaatgatag caaatcgcaa tttagcgttc cggcggactc gaacatgctc
     4441 ggcgctctcg gaactgccag aagcgtcgtc tgattccact tgccggaggt catgacacgt
     4501 cgtggtgcgg tggctgggcg gggagccagg cggatgtccg cgtaccaata cttgcagcaa
     4561 cctggacgag agacgaccca gacgaacggc gccaaggagg accgcacacg ccacccctgt
     4621 catcctgagc cgcagcgaag gatcgctcaa cgt
//
LOCUS       DASUGJ010000736         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_956074, whole genome shotgun sequence.
ACCESSION   DASUGJ010000736 DASUGJ010000000
VERSION     DASUGJ010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956074"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFV93_16640"
     CDS             complement(<1..394)
                     /locus_tag="VFV93_16640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5167037.1"
                     /translation="MPAEDCCGHGADEALLVRAAQADPAAFAPLYHAYYPRVYRYLRA
                     RSGSTEDAADLTQTVFLRALAALPDYRERGLPFAAWLFRIARNIVTDAQRRQRPTVDL
                     DSVPQERYATGANPPEQAAIDRERLDRLG"
     gene            535..1155
                     /locus_tag="VFV93_16645"
     CDS             535..1155
                     /locus_tag="VFV93_16645"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HEX5167038.1"
                     /translation="MFVFLDESGDPGFRMDKTSTRFFVIATVIVSDPFILDDAIDRFR
                     AEIGYTRNPEFKAASTPPAIRERFLRAICQWDFVVRALVVDKQRVTEPSLQSSETLYD
                     YFVAQLFDDDDDLIRDATIVLDESMKHRSAQKRAAGRLRRHLNRSSPQRVRQVRYRDS
                     KGSNALQAADMVAYSIYHRYRHSDDRYYRIIQPRTRIWELGGMDDP"
     gene            1328..1990
                     /locus_tag="VFV93_16650"
     CDS             1328..1990
                     /locus_tag="VFV93_16650"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5167039.1"
                     /translation="MRIERPGVRTGYDLWAETYDITPNPVVAIDARVTAAAVAAQAGE
                     RVLDAGCGTGRNLPGLLASGAAVVGMDFSSGMLAVARRNHPDVPLVLADMQVGWPFRD
                     GTFDVVLCALVGEHLDRLAAVTREMRRVLAPAGRAVFSVYHPAMAEAGKEANFQHAGT
                     EYRLGAVRHSLADYITAFEQAGFTTIRVDEYSGDDDLARVEPVAAAFIGFPLLVVITG
                     KA"
     gene            complement(2042..>2411)
                     /locus_tag="VFV93_16655"
     CDS             complement(2042..>2411)
                     /locus_tag="VFV93_16655"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX5167040.1"
                     /translation="DNDFPLGAGGELLQLGHDIESARSTNREHCHDEDQLLYAATTNR
                     ILLTHNRRDLRLLHDAWHRWGRAWSVQHQHAGILVLPQGRTYREHVTRVLALLGQNES
                     VTNQLHICGGSGDWRRYPDP"
BASE COUNT          441 a          729 c          782 g          459 t
ORIGIN      
        1 cgccgagccg gtcgagccgt tcgcggtcga tcgcggcctg ctcgggcggg ttcgcgccgg
       61 tggcgtagcg ctcctgcggc acgctgtcga gatcgacggt tggccgctgc ctgcgctggg
      121 cgtcggtgac gatgttgcgg gcgatccgga acagccatgc cgcgaagggc agcccccgct
      181 cgcggtagtc cggcagggcc gccagcgccc gcaggaagac ggtctgggtc aggtcggcgg
      241 cgtcctcggt cgagccgctt cgggcgcgca ggtagcgata gacgcgcggg taataggcgt
      301 ggtagagcgg cgcgaaggcg gctggatcgg cctgggcggc ccgaacgagc agcgcctcgt
      361 ccgcgccatg cccgcaacag tcctccgccg gcaactcggc cctcacctga cgcgcgccct
      421 tcctgctgtg ctgtctcgcc ctgtcatgag tgataacgtg tgacgcgcaa gatggggaca
      481 ggctgacgcg ctcacgttgg ctgtatcgcg aatgcagcgt gctatgatcc gccgatgttc
      541 gtgtttctcg atgaatcagg cgatccaggg ttccggatgg acaaaacctc aacgaggttc
      601 ttcgtcatcg ccaccgtgat cgtttccgat ccattcattc tcgatgatgc cattgatcgc
      661 ttccgagcag aaattgggta cacccgcaat cctgagttca aagcggcgtc gacgccacca
      721 gcgatccgag agcgatttct gcgcgcgatc tgccaatggg atttcgttgt ccgggcgctt
      781 gttgtcgata agcagcgtgt aaccgagcca tcgctccagt cgagcgagac tctctacgac
      841 tacttcgttg ctcagttgtt tgatgatgac gacgacctga ttcgcgatgc gaccatcgtt
      901 ctcgacgagt cgatgaaaca tcgatccgcg caaaagcggg ctgccgggcg attgcgacga
      961 cacctcaacc gaagctcgcc gcaacgagtt cgtcaggttc gatatcgaga ctccaaggga
     1021 agtaatgcgc ttcaggcagc cgacatggtc gcgtattcga tctaccatcg ctatcgccac
     1081 agcgacgatc gctactaccg catcattcaa ccgcgaacca gaatctggga actgggaggt
     1141 atggatgacc cctgagctag cctgatgttg tcatcaggca cgccaccagt cggcaacggt
     1201 tcgctcaagg gtcagaggat cgattcaatg tcatctgtcg cactgcgacc gctaatattg
     1261 tactccaaac gccttgcctt aactatggca gcgacaccaa tatcacagca catcatggag
     1321 aaagatcatg cggatcgagc gcccgggggt gcgaacgggg tatgacctgt gggcggagac
     1381 gtatgacatc acacccaatc cggttgtcgc gatcgacgcg cgggtgacgg ccgcggccgt
     1441 ggcggcgcag gccggcgagc gggtgctgga tgccggctgc gggacagggc gcaacctgcc
     1501 ggggctgctg gcgagcgggg ctgcggtcgt tgggatggat ttctcgtcgg gcatgctggc
     1561 ggtcgcgcgg cggaaccacc cggatgtgcc gctggtgctg gccgacatgc aggttggctg
     1621 gccgtttcgg gacggcacgt tcgatgtcgt cctctgcgcg ctggtcggtg agcacctgga
     1681 ccggctggcc gccgtaacgc gggagatgcg acgcgtgctg gcgccggccg gtcgggcggt
     1741 cttctccgtc taccacccgg cgatggccga agccggcaaa gaggccaact tccagcatgc
     1801 gggaaccgag taccggctcg gcgctgtccg ccacagcctg gccgactaca tcaccgcctt
     1861 cgagcaggca ggtttcacca cgatccgcgt cgatgagtac agcggcgacg acgatctggc
     1921 gcgcgtcgag ccggtcgcag ccgcgttcat tggcttcccg ctgctggtgg tgatcacagg
     1981 aaaagcctga agacgtcaag atttgtagcc cgagatggcg agccactcgt cgtgtcctgc
     2041 atcacggatc cggatatcgt cgccagtcgc cgctgccgcc gcaaatgtgc aattgattgg
     2101 ttaccgattc gttctggccg agcagcgcga gaacgcgcgt gacatgctcc cgatatgtcc
     2161 gtccctgtgg aagcaccaga atgccagcat gctgatgctg gacggaccac gcacgacccc
     2221 agcggtgcca cgcatcgtgc aacaagcgca aatcacgtcg gttgtgagtc agaaggatgc
     2281 gattggtggt cgctgcgtag agcagctgat cctcatcatg acaatgttct cgattcgtcg
     2341 accgtgcaga ttcgatgtca tgaccaagtt gtagaagttc gccgcccgcg ccgagcggga
     2401 agtcattgtc g
//
LOCUS       DASUGJ010000737         4237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1789689, whole genome shotgun sequence.
ACCESSION   DASUGJ010000737 DASUGJ010000000
VERSION     DASUGJ010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789689"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VFV93_16660"
     CDS             complement(<1..1033)
                     /locus_tag="VFV93_16660"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5167041.1"
                     /translation="MMNPNGAVLRAHAEQQFAEELAALAQVDNRQRPPSWRLSPWAVK
                     TYLLGGTLPNGAAISPKYIGNPRLVEIAVATLATDRALLLYGLPGTAKSWVSEHLAAA
                     ISGDSTLLVQGTAGTDESTIRYGWNYARLIAEGPSREALVSSPVLRAMEDGKIARVEE
                     LTRIPSETQDTLITILSEKTLPVPELGSEVQARKGFNLIATANNRDRGVNELSSALTR
                     RFNTVVLPTPATIEEEVGIVTRRAADLGRALELPAEPPALDEIRRVVTIFRELRDGVT
                     LDGQTKLKSPSGTLSPAEAISVVTGGLASAAYFGDGVLRAGDLASGLVGAIVKDPVQD
                     RVVWLEYLQT"
     gene            complement(1030..2580)
                     /locus_tag="VFV93_16665"
     CDS             complement(1030..2580)
                     /locus_tag="VFV93_16665"
                     /inference="COORDINATES: protein motif:HMM:NF039502.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HEX5167042.1"
                     /translation="MNDLLAVAVAGTARAGDALGSPAAPIDRAVAQLDAAGAERRVLL
                     AAGAHAIRRRAGVLPLAGAGTIDAADDETWQVAPQRIALLIAELLSGQAADVLPEALD
                     RLQRAQILLPPALLPAALDAGTRSRVVRPGLLAVIGQRGRWLARHNAAWAWATTRTDD
                     ELAARTDAIWQEGKQAERLAVLRLVRAGDPARAREMLETTWARERATFRAEAIDILSA
                     GLTAADQPFLEAALDDRAQPVRERAEALLICIPGTAPARDAVERAAPLIGKRLGFQVV
                     VRPPDGTDGTERATLMTEAISRVPPSHWVTQIGKQPADLVQSVARDQDWGFAILNGWT
                     QAALTFSDADWAAALLPAWFTAPPLGMAAKTYAGSYDATIHQHLIALIQIARPADAER
                     TVAEALSSAFNPIRITAVLPQLARPWSAGFSDRYLRSYRDLAEKAIKQSQVNWQASGI
                     WMTSFNTAALAICSGNIQSAIALLNHLSDITPSQGIDYRWLHMKQMLPLFIDALQMRQ
                     RIIEEIPG"
     gene            complement(2580..3908)
                     /locus_tag="VFV93_16670"
     CDS             complement(2580..3908)
                     /locus_tag="VFV93_16670"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEX5167043.1"
                     /translation="MELTVEQALALAPDASSASAGRKLARPGDWRNLGRNEVALWGEC
                     QGSALYQTRVDLRDYATRCSCPSRKFPCKHALGLLVLAAATPDLVPSAGAPEWVESWL
                     IQREKRAARTDGDTETKPATTTKTPPRQREDRRLARVQAGVDALNLWLDDLIRNGLAQ
                     VGMQPARFWEQQAARLVDAQASGLASRVRRLAAIPNASADWPSRLLDELGKLALLTHA
                     FGRIDELDPALRDDVRTATGWTIERDEVIERGETVTDDWLVIGQRVVAEERLRAQRTW
                     LLGTRSGRSALVLQFAHGTAPFTEAFAPGTRFGADVAFFPSALPLRAIVRERHGSPVA
                     IRQDDACELGGSIDAVLASWAAALARQPWIERIPVVLRSVVPVIEEAGDWVVRDDAGR
                     ALALEGAPHWSLLAHSGGRPLDLTAEWDGQALAPLGVFVAGAWQSLPEAG"
     gene            complement(4018..>4237)
                     /locus_tag="VFV93_16675"
     CDS             complement(4018..>4237)
                     /locus_tag="VFV93_16675"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5167044.1"
                     /translation="IGVRLPVRSDGLTAREIEVLRLIATAMSNRQIAETLFISPRTVE
                     RHIANIYLKLDIHSKADATAYAQCHHLV"
BASE COUNT          608 a         1463 c         1504 g          662 t
ORIGIN      
        1 ctgtctggag gtattcgagc cagacgacgc ggtcctggac cgggtccttg acgatcgcgc
       61 cgacgaggcc ggaggcgaga tccccggccc gcaggacgcc gtcgccaaag taagcggccg
      121 aggccagacc gccggtgacg accgagatgg cctcggccgg tgagagcgtg ccgctcgggc
      181 tcttcagctt ggtctggccg tcgagcgtga cgccgtcgcg cagctcgcgg aagatcgtca
      241 cgacccggcg gatctcgtcc agcgcaggcg gctcggcggg tagttccagc gcccggccga
      301 ggtcggccgc acggcgggtg acgattccga cctcctcctc gatcgtggcc ggtgtcggca
      361 ggacgaccgt gttgaagcgg cgcgtcagcg cactcgacag ttcgttgacg ccccggtcgc
      421 ggttgttggc ggtcgcgatc aggttgaagc ccttcctggc ctggacctcg ctgccaagct
      481 cgggcaccgg cagcgtcttc tcggagagga tcgtgatgag cgtgtcctgt gtctcggacg
      541 ggatgcgggt caactcttcg actcgcgcga tcttgccgtc ttccatcgcc cgcaggaccg
      601 ggctggagac gagcgcctcg cgcgacggcc cctcggcgat cagccgggcg tagttccagc
      661 cgtagcggat cgtgctctcg tcggttccgg ccgtgccctg gaccagcagc gtcgagtcgc
      721 cggagatggc tgccgccagg tgctcggaga cccacgactt ggccgtgccg ggcaggccgt
      781 agagcagcaa ggcccggtcg gtcgccagcg tcgcgacggc gatctcgacc agccgcgggt
      841 tgccgatgta cttcgggctg atcgccgcac cgtttggcag cgttccgccc agtagatagg
      901 tcttcacggc ccacggcgag agacgccagc tggggggccg ctgccggttg tcgacctgcg
      961 ccagcgccgc cagctcctcg gcgaactgct gctcggcgtg cgcccgcagg acggcgccgt
     1021 tcgggttcat catcctggta tctcctcaat gatccgttgt cgcatctgca gcgcgtcgat
     1081 gaatagcggt agcatctgct tcatgtgcag ccagcgatag tcgatcccct gagatggggt
     1141 gatgtcgctc agatggttca gcagcgcgat cgcgctctgg atgttgccgg agcagatcgc
     1201 gagcgcggct gtgttgaacg acgtcatcca gatgccggat gcctgccagt tcacctgcga
     1261 ctgcttgatc gccttctcgg ccaggtcacg gtacgagcgc aggtagcggt cgctgaaccc
     1321 ggcgctccag ggacgcgcta gctggggcag gacggcggtg atccggatcg ggttgaacgc
     1381 gctggagagt gcctccgcga cggtccgctc ggcgtcggcc ggccgcgcga tctggatcag
     1441 ggcgatcagg tgctgatgga tggttgcgtc gtacgagccg gcgtaggttt ttgccgccat
     1501 cccaagcggc ggggctgtga accaggccgg cagcagcgcg gcggcccagt ccgcatcgct
     1561 gaaggtgagc gcagcctgcg tccagccgtt gaggatggcg aagccccagt cctgatcgcg
     1621 cgcgacagac tggacgaggt cggccggctg tttcccgatc tgcgttaccc aatgcgatgg
     1681 cggtacgcgg ctgatcgcct cggtcatgag cgtggcacgc tcggtaccat cagtaccgtc
     1741 cggtgggcgg acgacaacct ggaaccccag ccgcttgccg atcagcggcg cggcccgttc
     1801 gacagcgtca cgggcaggcg ccgtgccggg gatacagatc agcaacgcct cggcgcgctc
     1861 gcgaaccggc tgggcccggt cgtcgagggc ggcttcgagg aacggctggt cagcggcggt
     1921 caggccagcg ctgaggatgt cgatcgcttc ggcgcggaag gtcgcccgct cgcgcgccca
     1981 ggtggtctcg agcatctccc gcgcgcgggc cggatcaccg gcacggacga gccgcaggac
     2041 ggccagccgc tcggcctgct tgccctcctg ccagatagcg tcggttcgcg cggcaagctc
     2101 atcgtcggtc cgcgtggtcg cccaggccca ggccgcgttg tggcgcgcca gccagcgccc
     2161 ccgctggccg atgaccgcca gtagccccgg ccggacaacc cggctgcgcg tccctgcgtc
     2221 gagggcggcc ggcagcaggg cagggggcag cagtatctgc gcccgctgga gccggtcaag
     2281 cgcctccggc aggacatcgg cggcctgtcc agacagcaat tcggcaatca gcaacgcgat
     2341 ccgctgcggt gcgacctgcc aggtctcgtc atcggcagcg tcgatcgttc ccgcgcccgc
     2401 cagcggcagg actccggcac ggcggcggat ggcgtgcgcc ccggccgcca gcagcacgcg
     2461 ccgttcggcg ccggccgcgt cgagctgcgc gacagctcga tcgatcggcg cggcgggcga
     2521 tccgagggca tcgccggcgc gggccgttcc ggcgacggca accgccagca agtcgttcat
     2581 cagccggcct ccggcagcga ctgccaggcg ccggcgacga agacacccag cggcgccagt
     2641 gcttgcccgt cccactcagc cgtcagatcg agcggccggc cgcccgagtg cgccagcagc
     2701 gaccagtgcg gcgccccttc gagcgccagc gcccgccccg catcgtcgcg gacgacccag
     2761 tcgcccgcct cttcgatgac gggcacgacg ctgcgcagga cgaccggaat ccgctcgatc
     2821 cacggctgcc gggcgagcgc cgctgcccag cttgccagca ccgcgtcgat tgatccgcca
     2881 agttcgcacg cgtcgtcctg ccggatcgcg acgggactgc cgtgccgctc gcggacgatg
     2941 gcgcgcagcg ggagggcact ggggaagaag gcgacgtcgg ccccaaaccg cgtgccgggc
     3001 gcgaacgcct ccgtgaacgg cgccgtgccg tgcgcgaact ggaggacaag cgcggatcgg
     3061 ccgctgcgtg tgccgagcag ccaggtccgt tgcgcgcgca agcgctcctc agcgacgacg
     3121 cgctggccga tgacgagcca gtcgtcagtg acggtttcgc cccgctcaat cacctcgtcc
     3181 cgctcgatcg tccagccggt ggccgttcgg acgtcgtcgc gcagggccgg atcgagctcg
     3241 tcgatccgcc cgaaggcgtg cgtcagcagc gccagcttgc cgagctcatc gagcagccgg
     3301 gagggccagt cggccgaggc gttcgggatc gccgccaacc ggcgcacgcg tgaggccagc
     3361 ccggaggcct gggcgtcgac cagccgggcc gcctgctgct cccagaacct ggccggctgc
     3421 atcccgacct gcgccagccc gttgcggatc aggtcgtcca gccagaggtt gagggcgtcg
     3481 acgccggcct ggacacgggc cagccgtctg tcctcgcgct ggcgcggcgg tgtcttcgtt
     3541 gtagtggcgg gtttcgtctc agtatcgcca tccgtgcgag ccgcccgctt ctcgcgctgg
     3601 atcaaccagc tctcaaccca ctcgggcgcg ccggcgctgg gaacgaggtc gggcgttgcg
     3661 gcggccagca ccagcaggcc gagcgcgtgc ttacagggga acttgcggct cggacaggag
     3721 cagcgcgtgg cgtagtcgcg cagatcgacg cgcgtctggt agagcgcgct cccctggcat
     3781 tcgccccaga gtgcgacctc gttgcgtccc agattgcgcc agtctcccgg cctggccagc
     3841 ttccggccgg cgctggcgga actcgcgtcg ggcgccagcg ccaacgcctg ttcgactgtc
     3901 aactccacga cggcgcctca cccatcgtcc ggacaacgct cgctcgtgcc gtccattcaa
     3961 tcagcaggcg ggggcgggtg gtagtcacca ttggtaccgg tgtcggggct gtcgctttca
     4021 gacgaggtgg tgacactggg cgtaggcggt cgcatcggct ttgctgtgga tatcgagctt
     4081 cagatagatg ttggcgatgt ggcgctcgac ggtgcgcgga ctgatgaaga gcgtctcagc
     4141 gatctggcga ttgctcatag ctgtcgcgat cagcctcaac acctcaatct cgcgcgcggt
     4201 aagtccgtcc gaacgcacgg gcaggcgaac tccgatc
//
LOCUS       DASUGJ010000738         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1790191, whole genome shotgun sequence.
ACCESSION   DASUGJ010000738 DASUGJ010000000
VERSION     DASUGJ010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790191"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VFV93_16680"
     CDS             <1..1143
                     /locus_tag="VFV93_16680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5167045.1"
                     /translation="RSPFLPFYIEDLGASSLESQALWAGIINAGGALMMAVSAPIWGI
                     VADRYGRKPMVLRAMFAGSVTIGLMSLATSPWHLLILRFIEGGVTGTVTASMTLVAST
                     TPKERMGFGLGMMQMAVFSGGSVGPLAGGILADQIGYRATFVVAGSMLFLGGLIVLTQ
                     VREQFTRTARGGTIDEDPAYRLRALLMGSSMLAMIAVMFTLRVASGAVQPIMPLYVET
                     LAGSASGVATLSGLTLGVAGLTSALASITLGRLADRIGQRPVLIVASFAVGLLYLPQA
                     FAQSALQLIVLQGLFGIAAGGILPSANAIVAHLTPPARRGAVYGFTAAATSVGGSLGA
                     LGGSGMTAAVDIRYVFIVSGLLMLGAAGWVMHAVRDVDEQPSAAPT"
     gene            complement(1957..>4179)
                     /locus_tag="VFV93_16685"
     CDS             complement(1957..>4179)
                     /locus_tag="VFV93_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167046.1"
                     /translation="ESPHRGYAFPSQQSEADFYGEDRSELITINKILVALDLIEIRKE
                     MLTRVDERGRRWRVPHNLYRVKDRPDGVDLRAVDVLRVAQLAQRDDAVFRYVRRVFSE
                     RFRPIDGDNVWHAILTELRNDATWRELQDRTRAIEKRASARTRAGHQSRAGGARTTQA
                     TPRPAPETVEASETDDALLSHTQHDGSSASFPATSVDVSNRGSNKPTETDVEPTNTGS
                     VVDVAASNTGSGRSGPSTAAAGNDGPAGVVGPSNTTYDQGLSTTTTTTTRLTDAGWDG
                     LRSSAAQPSHVPTSQERDSNTPASTLPRNRGRLALLRDDPIDEGDIGDGSIDPDSGQL
                     PVPAEMRGNGQARVATRMAGQAAACGQRETDEGVASGRDVAMVANGAAMVRPVADWNE
                     GDDDGQRAASARTGGGTGERRLADPAAGGPLVDPGPLVISTFEAANDRRATPLERHLL
                     AELECDADPPARAAGSTGADWVVAAMREAVASGSAFVAPKRIREIIARWASDARNAPS
                     SPSPGLSPAVDGASTGAGEAVADVRLPGGASGNAVWATVVGDLARVLDRDAHDRLLAG
                     SRIARYWRGTVEIRVTSLAAAGKLSNEYRGLVERHLNSRLRRPVAVRFEADADAEPDR
                     TPAAPSTDDEDAAGESPQPLRVAVAEAEIGRQVWQSLLGDLARRIPQTDLDRLAGVIV
                     LGQDAGGAIVLGAPSPLARRLLEGRYRAAVETSLAALLGQAAPLRILDAAGWCVDAAE
                     "
BASE COUNT          655 a         1370 c         1431 g          723 t
ORIGIN      
        1 cgttcgccct ttttgccttt ctatatagaa gacctcggcg catcatcgct ggaatcacaa
       61 gcgctctggg ccggcatcat caatgccggc ggggcactga tgatggcggt ctcggcgccg
      121 atctggggca tcgtcgccga ccgctacggg cgcaagccga tggtgctgcg ggcgatgttc
      181 gccggctcag tcacgatcgg gctgatgagc ctggcgacga gtccctggca tctgctgatc
      241 ctgcgcttca tcgagggtgg agtgacgggg acggtaacgg cctcgatgac gctggttgcc
      301 agcacgacgc caaaggagcg gatgggcttc gggttgggga tgatgcagat ggccgtcttc
      361 tccggcgggt cggtcgggcc gctggccggc ggcatcctgg ccgaccagat cgggtaccgg
      421 gcgacgttcg tcgtggccgg ctcgatgctg tttctgggcg ggctcatcgt cctgacgcag
      481 gtgcgcgagc agttcacacg gacggcgcgg ggcggcacga tcgacgagga tccggcctac
      541 cgactgcggg cactgctgat gggcagctcg atgctggcga tgatcgcggt catgttcacg
      601 ctgcgggtag cgtcgggcgc ggtccagccg atcatgccgc tctacgttga aacgctggcc
      661 ggctcggcgt ccggcgtcgc gacgctctcg gggctgacgc tgggtgtggc cgggctgacg
      721 agcgcgctgg cctcgatcac gcttggacgg ctggcggacc ggatcggcca gcggccagtg
      781 ctgatcgtcg cctcgttcgc ggtcgggctg ctctacctgc cgcaggcgtt cgcgcaatcg
      841 gcactgcagc tgatcgtcct gcaagggctg tttgggatcg cagccggcgg aatcctgccg
      901 agcgccaacg cgatcgtcgc ccacctgaca cctcccgcac ggcgcggcgc cgtctacgga
      961 ttcacggccg ccgcaacctc ggtcggcggc tcgctcggcg cgctgggcgg gtcggggatg
     1021 acggccgccg tcgatatccg ctacgtgttc atcgtcagcg gcctgctgat gctcggcgcg
     1081 gcgggatggg tgatgcacgc cgttcgcgac gtcgacgagc aaccttcggc agccccgacg
     1141 taacgacgct ggcaactgtt gccattgttc catgttcgta gggaccggtc tgatacccat
     1201 taccgaaaac tatcgatatc tggtgaagga tcggggagcc aggcagatca ggtgtgcgat
     1261 cggcggcacg ggtaacggcg tgaagcgggt actgcggacg gatcggggag catgccggga
     1321 cgggcagacg ctgggttggg gagggagcca ggcaacagcg cattcagatg cgaggtcgcc
     1381 gttgcggagg taggccgggc agtgtgcccg ttcggtcgtg agcgcgacgg gcgaagaggg
     1441 gagcccggcg gatctgccga ggtgttgggt gtcgagttgg gcgtgacgac ccgcccctgg
     1501 tgagacgcca cgacctcgct gactgctgcg agaggccaac cgaggttgtc gaggagcgtt
     1561 ccgcgacaag tagggcgggt cgtcgcgacc aactcgacgc accgaacgtt aagagacccg
     1621 ccgactcccc acctcgcacc ttgccctcgc cacaccgtgc gtcccgaccg gcttacccac
     1681 acggccaacc tgacgtgaca ctcgttccgc aaccccacgg ctccctcctc gcatgtcaca
     1741 ctcgccacac cgtgtatccc gccggctccc cacccaaccc cttcatcctc gtcacccacg
     1801 cacatccgct cagatcccct cggcaacggt ggcaacggca gcccacggat cgacacacat
     1861 ccatccggca accctgatcg acgccgatac gacgcgcaac tggcatgtgc aacaggagcg
     1921 gggatatgac gcatggtagg cgggatgggt gatgtgctat tcggcggcgt cgacgcacca
     1981 gccggcagcg tcgaggatgc ggaggggggc agcctgaccg agcagggcgg ccagcgaggt
     2041 ttcgacagcc gcgcggtagc gtccctccag gagccggcgt gccagtggcg acggcgcgcc
     2101 cagcacgatc gccccgccgg cgtcctgacc gaggacgatg accccggcca gccggtcaag
     2161 atcggtttgg ggaatgcggc gcgcgagatc gccgagcagc gactgccaga cctgccggcc
     2221 aatctcggct tcggccacgg caacccggag gggctgtggt gactcgccgg cggcgtcctc
     2281 gtcatccgtg gatggagccg ccggagttcg atccggttca gcatcagcat ctgcctcgaa
     2341 gcggacggca accgggcgac gcaggcgact gttcaggtgg cgctcgacca gcccgcgata
     2401 ctcgttggag agcttgccgg ccgcggcgag gctggtgacg cggatctcga ccgtgccgcg
     2461 ccagtagcgc gcgatccggc tgccggccag cagccggtcg tgggcgtcac gatcgaggac
     2521 gcgggcgaga tcgccaacga cggtcgccca gacagcgttg ccactggcgc cgcccggcag
     2581 ccgcacgtcg gcaaccgctt cgccagcacc ggtcgatgcc ccatcaacgg ccggactcaa
     2641 ccccggcgac ggggacgacg gcgcgttccg ggcatcgctc gcccagcgcg cgatgatctc
     2701 gcggatccgc ttcggcgcga caaaggctga tccagaggcg accgcttcac gcatcgcggc
     2761 gacgacccag tcggcgccgg tcgatccggc ggcacgagcc ggaggatcgg catcgcactc
     2821 cagttcggcg agcaggtgtc gttcgagggg ggtggcgcgc cggtcgttgg cggcttcgaa
     2881 ggtggaaatg accagtggac ctggatcgac cagcggccct ccggcagcgg ggtcagccag
     2941 acgccgctct cctgtgccgc cgcctgttcg cgcgcttgcc gctcgttgtc catcatcgtc
     3001 cccctcattc caatcggcca ccggccggac catcgccgcc ccgtttgcaa ccatcgccac
     3061 gtcacgaccg cttgccaccc cttcatctgt ctcacgctga ccgcaggctg ctgcctgtcc
     3121 cgccattcgc gtcgccactc gtgcctgtcc gttgccgcgc atctcggcag gcacaggcaa
     3181 ctggcccgaa tccggatcga tcgagccatc gccgatatcc ccctcgtcaa tcggatcgtc
     3241 tctgagcagt gcgagccggc cacgattgcg cggcagggtg ctggccggcg tgttggaatc
     3301 tcgctcctgg gacgttggca cgtgtgacgg ttgggcagcg ctgctgcgga gtccgtccca
     3361 gcccgcgtcc gtcaggcgag tagtggttgt cgttgttgta gatagacctt ggtcatacgt
     3421 agtgttgctg ggaccaacaa cgcccgccgg accatcgttg ccggcagcag cagtgctagg
     3481 cccggaccgc cccgaaccag tgttggacgc ggcaacatcg actaccgaac cagtgttggt
     3541 tggttcaaca tcggtctccg ttggcttgtt cgaacctctg ttgctgacat caacactggt
     3601 tgctgggaac gacgctgacg agccatcgtg ttgtgtgtgg gacaacaacg cgtcatccgt
     3661 ttcgctcgcc tcgaccgtct ccggcgctgg gcgtggcgtt gcctgagtcg ttcgagcgcc
     3721 gcctgcccgg ctctggtgtc cggcgcgggt gcgagcactg gcacgcttct cgatggcgcg
     3781 agtacgatcc tgcaactcgc gccaggtcgc gtcgttgcgc agttcggtca ggatggcgtg
     3841 ccagacgttg tcgccgtcga tcgggcggaa ccgttctgag aagacacggc gcacatagcg
     3901 gaagaccgca tcgtcgcgtt gggcgagttg tgccacgcgg agaacgtcta ccgcacgtaa
     3961 gtccacgcca tcgggacgat ccttcacgcg atagagatta tgtggcacgc gccagcggcg
     4021 tccacgctca tcgacgcggg tcagcatctc cttgcggatc tcgatcaggt cgagcgcgac
     4081 gaggatcttg ttgattgtga tcagttcgga tcgatcttct ccatagaagt cggcctccga
     4141 ctgctggctg gggaaggcat agccgcggtg gggtgattc
//
LOCUS       DASUGJ010000739         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1510291, whole genome shotgun sequence.
ACCESSION   DASUGJ010000739 DASUGJ010000000
VERSION     DASUGJ010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510291"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFV93_16690"
     CDS             complement(<1..662)
                     /locus_tag="VFV93_16690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HEX5167047.1"
                     /translation="MATETTPTTSLTTSEVSFPVEGMTCASCVRRVERALEKTPGVVS
                     ASVNLATERATVAYDPANASLDDLRGAVEKAGYGVPAEEQTFDIDGMFCASCVRRVER
                     ALEKTAGVQSASVNLATEQATVRYLPGVASRDDLVKAVEKAGYAVREQVAETSAGDEE
                     DREAARVRRELLVMKVKIVASLTVSAIMMLLMFWPVWLLGDRPVDEMEDLYKPLFLMA
                     TP"
     gene            complement(827..1696)
                     /locus_tag="VFV93_16695"
     CDS             complement(827..1696)
                     /locus_tag="VFV93_16695"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5167048.1"
                     /translation="MSTETNEFPEDDEIEHDGHPVALLGRYALGALDPDEVELVARHV
                     QSCPACRAELAGYDEIVSLLPYAAPVQQVPLRARAGLLARLDEIGTSNEQQMVVLPAI
                     PREPRHPLAWLRSSASPRRLAFAAVPLIVILAIVGIMADVINDQQRQIATIESEQAAQ
                     QEAVARVMLDGSENAEPAEATFISSTTAPEARAKLIVNYETNSALILARNLPLVENGA
                     QYIAWLRLSTPDEYARAGVLTVAEDGRASLSVEPHDAIQNYSEVIVTLEANPESAIPT
                     GPQVMTASVVPAN"
     gene            complement(1725..2366)
                     /locus_tag="VFV93_16700"
     CDS             complement(1725..2366)
                     /locus_tag="VFV93_16700"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5167049.1"
                     /translation="MSEKPRRISPAPRTLEPPVAVDSTDEELIRRVRHGDTAALETLY
                     GRYGRVVYSFAVRIVGDGQTAEEILQEAFVRTWRQAETFRTMRGNYASWLLSITHNLA
                     IDELRKRQRRPQRVDGVDISETLYGLVDEATNVEEAAEAGALRERVAAALATLPDAQR
                     IAVELAYFNGLSQREIAAHLREPLGTIKTRLRLGMQKLKDALADERDEFEPRT"
BASE COUNT          383 a          801 c          818 g          441 t
ORIGIN      
        1 atcggcgtcg ccatcaggaa gagtggcttg tagagatctt ccatctcatc gaccggtcgg
       61 tcgcccagca gccagaccgg ccagaacatc agcagcatca tgatcgcgct gacggtcagc
      121 gaggcgacga tcttgacctt catgaccagc agctcgcgcc ggacgcgggc ggcctcacga
      181 tcttcctcgt cgccggcgct ggtctctgct acctgctcgc ggacagcgta accggccttc
      241 tcgacggcct tgaccagatc gtcgcggctg gcgacgcccg gcagataccg cacggtcgcc
      301 tgctcggtcg ccaggttcac cgaggccgac tggacaccgg cggtcttctc caacgcccgc
      361 tcgacacgcc ggacgcagga ggcgcagaac ataccgtcga tgtcgaaggt ctgctcctcg
      421 gccggcacgc cgtagccagc cttctcgacg gcgccccgca ggtcatcgag gctcgcgttg
      481 gccggatcgt aggcgacggt cgcccgttcg gtcgccaggt tgaccgaggc gctgacgacg
      541 cctggcgtct tctcgagcgc gcgctcgacg cggcgcacgc atgaggcgca ggtcatcccc
      601 tcgacgggaa aggagacctc gcttgttgtc aggctggtcg tcggtgtcgt ctcggttgcc
      661 atcccctggt cctctcggcg ggcgtcatca gcccatcgcg gcgacgatgc cgcgtctgta
      721 gctcaatgat accccctggg ggtatggatg gcaaggtgcg gtccaatgca atccggtagg
      781 ggcgatatcg caatatcgcc cctaccggat tggggtcgat cgagacctag ttggctggga
      841 cgacggaggc cgtcatgacc tgcgggccgg ttgggatggc cgactcgggg ttggcttcga
      901 gggtgacgat cacctccgag tagttctgga tcgcgtcgtg cggctcgacc gagaggctgg
      961 cgcggccgtc ttcggcaacc gtcaggacgc cggcgcgggc gtactcgtcg ggcgttgaga
     1021 ggcgcagcca ggcgatgtac tgagcgccat tctcgacgag cggcaggttg cgggccagga
     1081 tcagggcgct gttcgtctcg tagttgacga tcagcttggc gcgcgcttcc ggcgccgttg
     1141 tcgagctgat aaaggtcgcc tcggccggtt ccgcgttctc gctgccgtcg agcatgaccc
     1201 tggcgacggc ctcctgctga gcggcctgct ccgactcgat cgtcgcgatc tgccgctgct
     1261 ggtcgttgat cacgtcggcc atgatgccga cgatggccag gatgacgatc agcggaacgg
     1321 cggcgaaggc cagcctgcgt ggcgacgccg acgaccgcaa ccaggccagc gggtggcgcg
     1381 gctcacgtgg gatcgccggc aggacgacca tctgttgctc gttgctggtg ccgatctcgt
     1441 ccagccgggc cagcaggccg gcgcgcgccc gcagtgggac ctgctggacc ggcgccgcgt
     1501 acggtagcag gctgacgatc tcgtcgtagc cggccagttc cgcgcgacag gccgggcagc
     1561 tttgaacgtg ccgcgcaacc agttcgacct catcgggatc cagcgcgccg agagcgtagc
     1621 ggccgagcaa tgcgacgggg tgtccgtcgt gctcgatctc gtcgtcttcc gggaactcgt
     1681 ttgtctccgt gctcacgagt acccaccacc ttccagggga gcgtttacgt ccgcggttcg
     1741 aattcgtcgc gctcgtcagc aagcgcgtcc ttcagcttct gcatacccag ccggagccgg
     1801 gtcttgatcg ttcccaatgg ctcacgcagg tgggcggcga tttctcgctg cgacaagcca
     1861 ttgaagtagg ccagctcgac ggcgatccgt tgcgcgtcgg gcaatgttgc cagcgccgct
     1921 gcgacccgtt cgcggagcgc gccggcttcg gccgcttcct cgacgttcgt ggcctcatcg
     1981 acgaggccat agagcgtctc gctgatatcg acgccgtcga cccgttgcgg gcggcgctgc
     2041 cgcttgcgca actcgtcgat ggcgaggtta tgcgtgatgc ttagcaacca gctcgcgtag
     2101 ttgccgcgca tcgtccgaaa tgtctcggcc tgtcgccagg tgcgcacaaa ggcttcctga
     2161 aggatctctt cggccgtctg gccgtcgccg acgatgcgaa ccgcaaacga atagacgacg
     2221 cggccatagc gaccgtagag cgtttcgagc gcggcggtat cgccgtgacg cacacggcgg
     2281 atcagctcct catcagtcga gtcgaccgcg acgggcggct cgagcgtccg gggagccgga
     2341 ctgatccggc gaggcttctc gctcatgagt cccctgccag tggccggcca aagcacactg
     2401 accaacccaa tatacgagcg agggaaccaa ttaggatgta cga
//
LOCUS       DASUGJ010000740         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_262669, whole genome shotgun sequence.
ACCESSION   DASUGJ010000740 DASUGJ010000000
VERSION     DASUGJ010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262669"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /locus_tag="VFV93_16705"
     CDS             <1..1574
                     /locus_tag="VFV93_16705"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5167050.1"
                     /translation="TLLILAAITMGLALAGQLVAIAETWAAEHVSWTATNALRADLVI
                     HLLRLDPAFHSAHTPGELIERVDGDVSALARFFSRFVVYVLGNAILVLAIVALLFVVD
                     WRVGLGLSVFVLLGLLAMLRIRAGATPAWAAERQSSADFYGFLGEYLAGTEDIRASGA
                     GPFVLRRFAEMMRPWFAATFRAGMWGYALMATSQGLVVLGTATAFALSAMLYRDGTLT
                     IGTVYLIVQYTVVLRQPTEQLRNEIQDLQQADASLGRVEALLQTVPRLTDGPGEALPP
                     GQLAVELEQVSFAYTDDLPALRDVSLRLEPGRVLGVVGRTGSGKTTLTRLLPRFYDPD
                     SGVVRLGGVDLRTVRIDAVRTRIGLVTQGIHLFNASVRDNLTLFDPSVPDVHLDAALD
                     AVGLSDWRRALPDGLDTMLGAGGVGLSAGQAQVLACARIFLRDPDIVILDEASSRLDP
                     ATERLVQSALARLLDGRTGVIVAHRLATLDLADDILVMEDGQVCEYGSRLALAADPTS
                     HFAGLLRTAGEEVLA"
     gene            1571..>2895
                     /locus_tag="VFV93_16710"
     CDS             1571..>2895
                     /locus_tag="VFV93_16710"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5167051.1"
                     /translation="MTEFTTWRITLKLARARPWLSLSHALLWTIIHASPLLPGLIALT
                     FFDTLTGEADPPGGTTGLILLLVAIGIVQVLLIFAAGYAETVMRFVLSGLLRRNLLEQ
                     VLRRLGADALPFSIGETISRFRDDAHEVEDNLDWVADVIGSGLFAVVAFLILLSVDAR
                     ITLVVILPLLAVVVVARRASDALGRRRAASSQATSEVTGAIGDILGAVQTLQAAGAEE
                     RAVAHFRRLNERRRVTMLADRILTQVLGAITANTVAIGTSLIMLLAASSLRDGSMTIG
                     EFVLFVTYLVYIADFTDGLGRFLAHYQQATVSVHRMSALLGGSPPDVLVEPNPLHLRG
                     TLPELPPAPTTTVRLALLEARGLTYHYPESGRGIDAVDLRLPRGTLTVVTGRVGAGKT
                     TLLRTLLGLLLAEAGEVCWNGERVSDPAAVLVPPRAAYTAQAPRLFSDTL"
BASE COUNT          396 a         1004 c          996 g          499 t
ORIGIN      
        1 ggacgctgct gattctggcg gccatcacga tggggctggc gctggccggt caactggtgg
       61 cgatcgctga gacgtgggcg gccgagcacg tcagctggac agcgaccaac gcgctgcgtg
      121 cagatctcgt catccacctg ctgcggctgg acccggcctt ccattctgcc cacacgcccg
      181 gagaactgat cgagcgggtg gacggcgatg tctcggcgct ggcgcgcttc ttctcgcgct
      241 tcgtcgtcta cgtgctgggc aacgcgatcc tggtcctggc catcgtcgcg ctgctgtttg
      301 tcgtcgactg gcgggtcggg ctggggttga gcgtcttcgt cctgctcggc ctgcttgcga
      361 tgctacgcat tcgcgccggc gcgacaccgg cctgggcggc cgaacgacag tccagcgccg
      421 acttctacgg cttcctcggc gaatacctgg ccggcaccga ggacattcgc gccagcgggg
      481 ccggcccgtt cgtcctgcgc cgcttcgccg agatgatgcg cccgtggttc gcggccacgt
      541 tccgggccgg tatgtggggt tacgcgctga tggcgaccag ccaggggctg gtcgtgctcg
      601 gcacggcgac cgcgttcgcg ctgagcgcga tgctgtatcg cgacggcaca ctgacgatcg
      661 gcactgtcta cctgatcgtc cagtacaccg tcgtcctgcg ccaaccgacc gagcagctgc
      721 gcaacgagat ccaggatctg caacaggccg acgccagcct gggccgagtc gaggcgctgc
      781 tccagacagt tccgcgactg actgatggcc ccggcgaggc gctgccgccc ggccagctgg
      841 cggtcgagct ggagcaggtc tcgttcgcct ataccgacga cctgcccgcc ctgcgcgacg
      901 tcagcctgcg gctggagccg ggacgcgtgc tcggcgtcgt tggacggacc ggaagtggca
      961 agacgacctt gacgcggcta ctgccccgct tctacgaccc cgacagcggc gtcgtccggc
     1021 tgggcggcgt cgacctgcgg acggtgcgaa tcgatgccgt gcgcacccgg atcggcctcg
     1081 tgacgcaggg catccacctc ttcaacgcca gcgtccgtga caatctgacg ctcttcgacc
     1141 cttcggtccc ggacgttcac ctcgacgcgg ccctcgacgc ggtcgggctg agcgactggc
     1201 gacgagcgct gccggatggc ctggacacga tgcttggtgc cggtggcgtc ggcctctcgg
     1261 ccggacaggc gcaggtgctg gcctgcgcgc ggattttcct gcgcgatccc gacatcgtga
     1321 tcctcgacga ggcgtcctcg cgactcgacc cagccaccga gcggctggtc cagtcggcac
     1381 tggcgaggct gctcgatggc cggaccggcg tcatcgtcgc gcaccggctg gcgacgctcg
     1441 atctggccga cgacatcctg gtcatggagg acggccaggt ctgcgagtac gggtcccgac
     1501 tcgcgctggc cgccgatccg acctcgcact tcgccgggtt actgcgaacc gccggagagg
     1561 aggtgctggc atgaccgagt tcacgacctg gcgaatcacg ctcaagctgg cccgcgcgcg
     1621 gccgtggctc tcactgtccc acgcgctgct gtggacgatc atccacgcct cgccgctact
     1681 gccggggctg atcgcgctga ccttcttcga cacgctgacg ggcgaggctg atcctcccgg
     1741 cggcacgacc gggttgatcc tgctgctggt cgcgatcggg atcgtgcagg tgctgctgat
     1801 ctttgcggcc ggctacgccg aaacggtcat gcgcttcgtg ctgtctggcc tgctgcgtcg
     1861 caacctgctg gagcaagttc tacggcggct gggcgcagac gcgctgccgt tctcgatcgg
     1921 cgagacgatc agccgcttcc gcgacgatgc ccacgaggtc gaggacaacc tggactgggt
     1981 cgcggacgtc atcggcagcg ggctgtttgc cgtcgttgcc ttcctgatcc tgctctcggt
     2041 ggatgcgcgg atcacgctgg tcgtcatcct gccgctgctg gccgtcgtcg tcgtcgcgcg
     2101 gcgggccagc gacgcgctgg gacgacgacg cgctgccagc agccaggcaa ccagcgaggt
     2161 caccggcgcg attggtgaca tcctcggcgc ggtccagacg ttgcaagccg ccggggcgga
     2221 ggagcgcgcc gtcgcccact tccgccgcct gaacgaacgt cgccgggtca cgatgctggc
     2281 cgaccggatc ctgacgcagg tactgggagc gatcacggcc aacacggtcg cgatcggcac
     2341 gagcctgatc atgctgctgg cggcaagtag cctgcgggat ggcagtatga cgatcggcga
     2401 gttcgtgctg ttcgtcacct acctcgtcta catcgccgac ttcaccgacg gcctcggccg
     2461 cttcctggcc cactaccagc aggcgaccgt ctcggtccac cgcatgtctg cgctgctggg
     2521 cggttcaccc ccggatgttc tcgtcgagcc gaacccgctc cacctgcgcg gcacgctgcc
     2581 ggagttgccg ccggcaccca cgacgactgt ccggctggcg ctgctggagg cgcgcgggct
     2641 gacctaccac tatccggagt cgggacgtgg gatcgacgct gtcgacctgc gcctaccgcg
     2701 cggcacgctg acggtcgtaa ccgggcgggt tggcgccggc aagacgacgc tgctacggac
     2761 gctgctggga ctgctgctgg ccgaagctgg cgaggtctgc tggaacggcg agcgcgtaag
     2821 cgatccagcc gctgtcctcg tgccgccccg cgcggcgtat acggcgcagg cgccgaggct
     2881 gttcagcgac acgct
//
LOCUS       DASUGJ010000741         5689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_121689, whole genome shotgun sequence.
ACCESSION   DASUGJ010000741 DASUGJ010000000
VERSION     DASUGJ010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121689"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..533)
                     /locus_tag="VFV93_16715"
     CDS             complement(6..533)
                     /locus_tag="VFV93_16715"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5167052.1"
                     /translation="MDKANVLARVESARAAFDRQLVEVGETRMEQPGVMGDWSVKDLL
                     AHIMAYDRWTAAQLNAAREGREATSLEAFGVAELPPGADSDDLDTRNAALRDYYRDAP
                     LADIIAGAEHAFGLLVAAIQELDQSQLDDPGFLGWGEDLTTTDCIAIQSWDHYEDHEP
                     QLRAWILRDEGLPNV"
     gene            complement(575..1804)
                     /locus_tag="VFV93_16720"
     CDS             complement(575..1804)
                     /locus_tag="VFV93_16720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5167053.1"
                     /translation="MTPPETASEQTGLSRRLMTALCLPASLAVLNALALSPFLSEIAK
                     DLDTSVAVLGQALTVTGLLGATFGLVIGPAADRIGYRRLMLCGLAALIICDLMTALAP
                     SFPAILLAQIVGGFSAATITPMAYSIIGTRVAGEGRRRAIATIYAASSAGNIIGFPVL
                     STIGDSFSWRWSFVAVAVAASFGLLLAAIELPRDQPVERSPLRLATVLATYRPLVTHE
                     RMRLIYAAQFLRGVAWTGMLTYIGAFATDELGASVRLTGFLWLMISPGFMAGSLLVGG
                     RLRRVNPRAAFALATTIMGALIGLMFIIQPTIEAAFGLLLVIALFAGIAEVAAAGIVA
                     SETPAPQGATMSLHSSVLRFGTATGALIGGGLIALGSFSLLGAGLPFIAALGGTVGWL
                     SGRPAHVPLPRGEPVRE"
     gene            complement(1801..2346)
                     /locus_tag="VFV93_16725"
     CDS             complement(1801..2346)
                     /locus_tag="VFV93_16725"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5167054.1"
                     /translation="MTPVEAPTIETARLRLRPLTEADVPEWSRLLYADPEVTRYLPGP
                     DVTPLERTERLFQHFSVHWQRHGYGEWAVTDRETGAFLGQCGLNHIDDLSETELDYAL
                     ARRAWGRGLATEAARAAVSYAFESAGLTRLIGFVVPEHVVSRRVLERLGFVYKCDMHY
                     WGVDLVRYGLQRDDPTAAEQR"
     gene            complement(2475..3026)
                     /locus_tag="VFV93_16730"
     CDS             complement(2475..3026)
                     /locus_tag="VFV93_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167055.1"
                     /translation="MESQQQWHTDDGLIRLWDRVGMPFIGDVTNPFTGSGAAERERVV
                     VVEPPVSLEDYLVERQLRPAAATIDELTIRQALMTLGLSERAHRYPEWVRAEAWLFMA
                     LRIERQVLAVDLLARAHDLGLSQSSIEQARVRLRVVTFRQPGIRHGGWRWQLPPSIGE
                     HSTPGDIDPRWRRYQQYRSPAQA"
     gene            complement(3162..4100)
                     /locus_tag="VFV93_16735"
     CDS             complement(3162..4100)
                     /locus_tag="VFV93_16735"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5167056.1"
                     /translation="MAGERARPLKVGMMLPQAEGRHGGGTAGWAEMLAMARRAEEIGF
                     DSIWTIDHFILAYPNQPQQGVWENWSLLAALAAATSRVELGPLVTPTSFHNPAMLAKI
                     VDTIEEISGGRLTLGLGAGWVEREYQAMGLPYDHRVSRFEEAVQIIHGLLRTGAVDFE
                     GRYYSARECELRPRGPRAGGPPILIGSSGERMLRIMARYGDAWNAFFSKTGNSPAGVA
                     PLRDAVDVACRAEGRDPTTLARTITAFVAFDLPNTTGHPDVTTPLRGTPDEIAAELRA
                     YAAEGISHIQIFLDPMTLAGVEAFAPVLEALDRRDG"
     gene            complement(4163..4627)
                     /locus_tag="VFV93_16740"
     CDS             complement(4163..4627)
                     /locus_tag="VFV93_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5167057.1"
                     /translation="MALYFLDTSALAKRYISEIGSGWVRTLCVSDSIVISAIALTEMS
                     SVFARRHREGAISISDRNALIRLFQAHVRQYALVDVDRTILNSAGALIIQAPATTAMR
                     SLDAIQLACGQAFVTRSAASNLGPLTFVSADLRLLAAAQWAGFATDNPDIQP"
     gene            complement(4634..4981)
                     /locus_tag="VFV93_16745"
     CDS             complement(4634..4981)
                     /locus_tag="VFV93_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167058.1"
                     /translation="MATLTIPDPLYTQLQAIAARRQRPVEDWLLEAIEKLAADVDEPA
                     GAPLTREEVLRKLREADPELIWSEDDVESLFDGLDLPPMSDEEAERIVAAIPPLDPPL
                     SQTIIDMREEERY"
     gene            5123..>5689
                     /locus_tag="VFV93_16750"
     CDS             5123..>5689
                     /locus_tag="VFV93_16750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5167059.1"
                     /translation="MADLIGTTIGNYRIESLLGTGGMGAVYRGVHLHLNRPAAVKVMH
                     AHLAGEESFKRRFRQEARAVAALSHPHIVQTWDFGVQDGNFYLVMELLNDGSLRSLLG
                     SDEARQPGWAIHVGLDLVRQAADGLAYAHRRGMVHRDIKPDNLLLERRPDDLDGDERN
                     DLAVKITDFGLARMTEDASATGPSLVMGT"
BASE COUNT          945 a         1798 c         1992 g          954 t
ORIGIN      
        1 ggcccctaca catttggaag cccctcgtcg cgcaatatcc acgcacgcag ctgcggctcg
       61 tggtcttcgt agtggtccca tgactggatc gcgatgcagt cggtcgtcgt cagatcttcg
      121 ccccagccga ggaagccggg atcgtccagc tgtgactggt ccagttcctg aatcgcggcg
      181 accagcagac cgaaggcgtg ctcggcgccg gcgatgatgt cggccagcgg tgcgtcgcgg
      241 tagtagtcgc gcagggcggc gttgcgggtg tcgaggtcgt ctgagtcggc gccgggcggc
      301 agttcggcta ccccgaatgc ttccagcgat gtcgcctcgc gaccctcgcg ggcggcgttg
      361 agctgcgcgg ccgtccagcg gtcgtaggcc atgatgtgcg ccagcagatc cttgaccgac
      421 cagtcgccca tgacaccggg ctgctccatc cgcgtctcgc caacctcgac cagctgccgg
      481 tcgaacgcgg ctcgcgccga ttcgacgcgg gcgagcacat tggccttgtc catcgtctgc
      541 ttccttgtcc ctcacgcgcg aagcggatca gccgctattc tcgcacgggt tcgccgcgtg
      601 ggagtggaac gtgggcaggg cgacctgaga gccagccgac ggttccaccg agtgcggcga
      661 tgaagggcag gccggcgcca agcaggctga agctgccgag cgcgatcagg ccgccgccga
      721 tgagtgcgcc ggtggctgtt ccgaagcgca ggaccgagga gtgcagcgac atcgttgcgc
      781 cctgcggggc cggtgtctcg ctggcgacga tcccggcggc ggctacttcg gcgatcccgg
      841 cgaagagggc gatgaccagc agcagcccga aagcggcttc gatggttggc tggatgatga
      901 acatgagccc gatcagcgcg cccatgatcg tcgtcgccag tgcgaaggcg gcgcgggggt
      961 tgacgcggcg cagccggccg ccgaccagca ggctgccggc catgaagccg gggctgatca
     1021 tcagccagag gaagcctgtc agccggaccg acgcgccaag ctcgtcggtg gcgaaggcgc
     1081 cgatgtaggt cagcatcccg gtccaggcga cgccgcgcag gaactgggcg gcgtagatca
     1141 ggcgcatccg ctcgtgcgtg acgagcgggc ggtaggtcgc gaggacggtc gcgaggcgta
     1201 gcggagatcg ctcgaccggc tggtcgcggg gcagttcgat cgcggccagc agcaagccga
     1261 acgatgccgc gacggcgacg gccacgaacg accagcgcca cgagaacgag tcgccgatcg
     1321 tcgagaggac cgggaagccg atgatgttcc cggccgacga ggctgcatag atcgtcgcga
     1381 tcgcacggcg acggccttcg ccggcgaccc gcgtcccgat gatggagtag gccatcggcg
     1441 tgatcgtcgc cgcgctgaag ccgccgacga tctgggccag caggatggcc gggaacgacg
     1501 gcgcgagggc ggtcatcagg tcgcagatga tgagggctgc cagcccgcag agcatcagcc
     1561 gccggtagcc gatccggtcg gcggccgggc cgatcaccag tccgaaggtc gcgccgagca
     1621 ggccggttac ggtcagtgcc tggccgagga cggcgaccga ggtgtcgagg tccttggcga
     1681 tctccgacag gaacggcgag agtgccagcg cgttgaggac ggccagcgag gccggcaggc
     1741 agagcgccgt catcaatcga cggctgagac ccgtctgctc ggaagcggtt tccggaggcg
     1801 tcaccgctgc tccgctgcgg tgggatcgtc tcgttggagt ccgtagcgca ccagatcgac
     1861 gccccagtag tgcatgtcgc acttgtagac gaagccaagc cgctccagca cccgccgcga
     1921 gacgacgtgc tcggggacga cgaagccgat cagccgcgtt agcccagccg actcgaaggc
     1981 gtagctgacg gccgcccgtg ctgcctcggt tgcgagaccg cggccccagg cgcgccgcgc
     2041 cagcgcgtaa tccagttccg tttcgctgag gtcgtcgatg tggttcaggc cgcactggcc
     2101 gaggaaagca ccggtctcgc ggtcggtgac ggcccactcg ccgtagccgt gacgttgcca
     2161 gtggacactg aagtgctgga agagtcgctc cgtgcgttct agtggagtga cgtccggtcc
     2221 cggcaggtag cgcgttacct ccggatcggc gtagagcagc cgcgaccatt cgggaacatc
     2281 cgcctccgtc agcgggcgta gccgcagccg cgccgtctcg atcgttggag cctcgaccgg
     2341 cgtcatccgt ctgtccctca accagcgccg gttcacggcg tcgttcgcac ggtgtctacg
     2401 gcgcagtatg gtaaccgacg ctggtggtgt tttgcggagg aagcatggat tgacgcggag
     2461 cgatacccga tcgcctaggc ctgtgcaggc gatcggtatt gctgatagcg tcgccagcgc
     2521 gggtcgatgt cgcccggcgt gctgtgttcg ccgatcgacg gcgggagttg ccagcgccag
     2581 ccgccgtggc ggatgcccgg ttgccggaac gtcacgaccc tgagccggac acgcgcctgc
     2641 tcgatgctcg actgtgacag cccgagatcg tgcgcccggg ccagcaggtc gactgccagc
     2701 acctgccgct cgatccgtag tgccatgaag agccacgctt cggctcgaac ccactcgggg
     2761 tagcggtgcg cccgctcgct gagcccgagc gtcatcagtg cctgacggat cgtgagctca
     2821 tcgatcgtgg ctgccgccgg tcgcagttgg cgctcgacca ggtagtcctc cagcgagacc
     2881 ggcggctcga cgactacgac gcgctcacgc tcggccgcgc cgctaccggt aaagggattg
     2941 gtgacatccc cgataaacgg catcccaacc cggtcccaca gccgaatcaa cccgtcgtcc
     3001 gtatgccact gctgctggct ctccatggta tcgcctctca ggtggaatag aacataggtt
     3061 ctactcagtg ggtatgctac gggtcaggct agcgaaagtc aagggtgttt tcgtgacggg
     3121 tgacgcgtga tgcggaggtt cgtcgcgtca cgcgtcaccc gtcacccgtc acgacgatcc
     3181 aacgcttcca gaaccggcgc gaacgcttcg acgccggcaa gggtcatcgg gtcgaggaag
     3241 atctggatgt ggctgatgcc ttcggcggcg taggcgcgca gttcggcggc gatctcgtcg
     3301 ggcgtgccgc gcaggggtgt tgtcacgtcc gggtggccgg tcgtgttggg caggtcgaag
     3361 gcgacgaacg ccgtgatggt gcgggcgagc gtggtagggt cgcggccctc ggcgcggcag
     3421 gcgacatcga ccgcgtcgcg gagcggggcg acgccggccg ggctgttgcc ggtcttactg
     3481 aagaaggcgt tccaggcgtc gccgtagcgc gccatgatcc gcagcatccg ctcgccactg
     3541 gagccgatca ggatcggcgg gccgccggcg cgcgggccgc gtggacgcag ctcgcactcg
     3601 cgggcggagt agtagcggcc ttcgaaatcg accgcgccgg tccggagcag tccgtggatg
     3661 atctggacgg cctcctcgaa acgcgagacg cggtggtcgt agggtagccc catcgcctgg
     3721 tactcgcgtt cgacccagcc ggcgccgaga ccgagggtca gccggccgcc cgagatctct
     3781 tcgatggtat cgacgatctt ggccagcatg gccgggttgt ggaacgaggt cggcgtcacc
     3841 agcggcccca gctcgacccg cgacgtggcg gcggccagcg ctgccagcag cgaccagttc
     3901 tcccagacgc cctgctgtgg ctggttgggg taggcgagga tgaagtggtc gatcgtccag
     3961 atcgagtcga agccgatctc ttccgctcgg cgggccatcg ccagcatctc ggcccagccg
     4021 gccgtcccgc cgccatgccg cccctcggcc tgcggcagca tcatgccgac cttcagcgga
     4081 cgtgcccgtt ccccagccac ggcagacccc tctcctgcat gcccagcgac atcgctcggg
     4141 agaggatact cgcgattggc tgttacggtt ggatgtcggg gttgtcggtc gcgaatccgg
     4201 cccactgagc tgccgcgagg agacgtaaat cggcggaaac gaatgtcaac ggaccgaggt
     4261 tgcttgcagc actgcgtgtg acgaacgctt gcccacaggc gagctggatt gcgtccaacg
     4321 atcgcatggc cgtcgtcgcg ggtgcctgaa tgatgagcgc gccagcgctg ttgaggatcg
     4381 tgcgatcgac atccacgagc gcgtactgcc tgacgtgggc ttgaaacagg cgaatgagcg
     4441 cattcctgtc ggatatcgaa atcgcacctt cacggtgacg acgagcaaag accgaactca
     4501 tttcggtgag tgcgatggcg gagatgacaa tggagtcgga gacgcagagt gtgcgcaccc
     4561 atccggaacc gatctccgag atgtaccgct ttgcaagcgc actcgtgtcg agaaagtaca
     4621 acgccatcga cggtcagtac cgttcctctt cgcgcatgtc gatgattgtc tgcgatagcg
     4681 gtgggtcgag tggtggaatc gccgcgacga tccgttcagc ctcctcatcc gacatcgggg
     4741 gcaggtcgag gccgtcgaat aaggactcga catcatcttc ggaccagatg agttccggat
     4801 cggcctctcg gagcttacgt agcacttctt cgcgtgtcag gggggcgccg gctggttcgt
     4861 caacgtcagc agcaagtttc tcgatcgctt caagcagcca gtcttccacc ggtcgttgtc
     4921 gacgagcggc gatggcttgg agttgcgtgt agaggggatc ggggatcgtc aatgttgcca
     4981 tcgtgattgt ccttcatcag cctgtcgtgt tcacgcatat tgaacacgcc tgtcgtgtcc
     5041 cggcactgat attgcctgat cccggccgtg ggtgactgcc ggtcacccga tcgtggcaat
     5101 tggagtgcgt gagagcgacg gcgtggcaga tctgatcggg acgacaatcg gcaactaccg
     5161 gatcgagtcg ctgctcggca cgggggggat gggggcggtc tatcgtggcg tgcacctgca
     5221 tctcaaccgg ccggcggccg tcaaggtcat gcacgcccac ctcgccggcg aggagtcgtt
     5281 caagcggcgc ttccggcagg aggcgcgggc ggttgcagcg ctgagtcatc cgcacatcgt
     5341 ccagacctgg gacttcggcg tccaggacgg caacttctac ctggtgatgg aactgctcaa
     5401 cgatggctcg ttgcgctcgc tgctggggtc cgacgaggcg cgccagccag gttgggcgat
     5461 ccatgtcgga ctcgacctgg tccggcaggc agccgatggg ctggcctacg cgcaccggcg
     5521 cgggatggtc catcgcgata tcaagccgga caacctcctg ctcgaacgcc gtccggatga
     5581 tctcgacggc gatgagcgca acgacctggc ggtcaagatc accgacttcg ggctggcacg
     5641 gatgaccgag gacgcctcgg cgaccgggcc gagcctggtg atggggacg
//
LOCUS       DASUGJ010000742        11735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_678633, whole genome shotgun sequence.
ACCESSION   DASUGJ010000742 DASUGJ010000000
VERSION     DASUGJ010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678633"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFV93_16755"
     CDS             <1..510
                     /locus_tag="VFV93_16755"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HEX5167060.1"
                     /translation="RGEFDNLAGQGKPQDLTTDHHAGDRESGFRVLKNNGPAPAWIEA
                     DKAIRAEQAALAELAARTASFITEHLDDETTNAPAPAAPTFSARLRRLWSGSRRPAQP
                     VNHFTRADLRNERDRVRREYLRRSDELNQTINEYNSALPFDLLWRERQKITPEQAAAT
                     FNSACPPVG"
     gene            725..1063
                     /locus_tag="VFV93_16760"
     CDS             725..1063
                     /locus_tag="VFV93_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167061.1"
                     /translation="MPDTWETLAQAIEEQRQVLATYNGRPRAFCPHALGMKGDKRHVL
                     VYQFDAARRIGERPTTGWRCLEVDRLEDVSLHIGEWHTAPNVFNPQTCLDQIDVVVSP
                     FQQRRQPAAR"
     gene            complement(1071..1397)
                     /locus_tag="VFV93_16765"
     CDS             complement(1071..1397)
                     /locus_tag="VFV93_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167062.1"
                     /translation="MHKWEYKVLHRVRGVGVNRIGGWDEPIVSELPALGDEGWELVTV
                     ITRSSEPGSATSGVTSDEMWVFKRLKSSARSETAVITTQAVPQPAPAIEDMDVITRAE
                     AIEHEE"
     gene            1640..2764
                     /locus_tag="VFV93_16770"
     CDS             1640..2764
                     /locus_tag="VFV93_16770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HEX5167063.1"
                     /translation="MGAGTAVLPRKPVLAESDFSRQPFVLAWELTRACNLACVHCRAE
                     ALLRRDPRELSTAEALAVIDDIAGFDVPPVLILTGGDPMRRPDLNDLIRHATERNVRC
                     TLTPAGTALTSERRLAEAKEAGLSRIAVSLDGPTAEEHDSFRRVKGSFGWSLDILSAA
                     ERLQIPVQIHTTLCKRTLHLMPAMADLAEQLGAVVWAIFCLVPTGRGVDLDELTADEY
                     EMIFEWLVERSATASWNLKLTEGYHYRRVLIQHRQAPVAGPGFQSSDGIGRATRAVNA
                     GNGFCFISHTGDICPSGFLPVVAGNVRNDSVVDVYRDHRVFRELRDPSLLAGKCGHCE
                     FRDICGGSRSRAFARTGDYLESDTACAHMPGDGAALSFRN"
     gene            2884..4794
                     /locus_tag="VFV93_16775"
     CDS             2884..4794
                     /locus_tag="VFV93_16775"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEX5167064.1"
                     /translation="MVQTQVRNVLEPLSAEEVRAAVRILKSETTLPEKHRFIQVALRE
                     PDKEQALAALAGSNGHLPDREAAVVLLDHDRRSTREGVVSISAGTVVSWNELPDVQSP
                     IAMEEFFECEKACKADPAFREALAKRGVTDMDLVMVDPWSAGHYEDNEGRRLSRALVW
                     VRADPEDNGYAHPVENLIVLVDLHEMAVYRIEDFGVVPIPWADGNFAPKYTGEPRTDL
                     KPIEITQPEGVSFTVEGHEVAWQNWRFRVGFTPREGLVLHTVGWDDGDSLRPILYRAS
                     LAEMVVPYGDTNITQARKNAFDVGEYNIGLLANSLELGCDCLGEIYYFDAVVCDNRGE
                     PMTITNAICMHEEDASLLWKHSDFRIGTSEVRRSRKLVVSFISTVGNYDYGFYWSFFQ
                     DGTIHFEIKQTGIVTTAVTESSEPLKYGQLLNTGGLYAPIHQHFYNIRLDMDVDGTDN
                     SLYEVHAERVPAGADNPYNNAFYSKSTLLRRESEAQQDIDPMTGRVWKIVSNSRTNAV
                     GEPTAYRLVPHTNVAAFAAPEASVTKRAGFITKHLWATPWQRNENFPAGDYPNQHPGG
                     AGLPEWTSADREIENTDLVLWYTLGAHHPPRLEDWPIMPVTGASFVLQPAGFFDRNPT
                     LDVAPPKGHCHP"
     gene            complement(4858..5745)
                     /locus_tag="VFV93_16780"
     CDS             complement(4858..5745)
                     /locus_tag="VFV93_16780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5167065.1"
                     /translation="MSAGKASITSAGNVTVRPLRPGPSLGTRRIVASPLALASLAFIV
                     VLALAAVLSPLIVPHDPRLIAPAIRLQPPSADHLLGTDELGRDVFSRLIDGGRVSLLV
                     GAVVTAVSAALGTFFGVVSGYYRRLDPLIMRVMDGFMAFPGVLLAIAVVISFGARTSS
                     VILALSLVYIPTIARLMRSTTLLIRELPYIESARAIGQTDLRILWRYVLANSLSPLIV
                     QCTFIAAYAILAEASLAFLGAGGDPQAPSWGNMLRDGQRLLGPAWWIAVAPGTLLFLT
                     VLALTVIGDRLRDALDPRR"
     gene            complement(5732..6688)
                     /locus_tag="VFV93_16785"
     CDS             complement(5732..6688)
                     /locus_tag="VFV93_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5167066.1"
                     /translation="MIGLVVRRLLALVPVALIVGTITFTLLHVAPGDPAAVMLGSEAT
                     PEQVARARERLGLDDPLLVQYPRWLLGILRLDFGDSLFLDRPVATAIAGRIVPTLQLT
                     LYAMVIALVIGVPAGVIAAVRQNSLIDRLLMLLAVAGTAIADFFLAILLVLLFAVTLR
                     WLPSGGYVPLLDSPIDHARSMVLPAFALGLSIAGLPARLIRASLLDVLQEDYVRAAIA
                     RGLSPATVVVRHALRNALLPAVTVLGYTLGDLLGGAVVVETVFNLPGMGQLVVNSIAR
                     RDFPVIQGVVMAIAGCYLLANLLVDVLYMVIDPRIRHGHVSR"
     gene            complement(6685..8331)
                     /locus_tag="VFV93_16790"
     CDS             complement(6685..8331)
                     /locus_tag="VFV93_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5167067.1"
                     /translation="MVRDDLLHRRLSRRTLLRAAAGIGLAIPATGLLAACGGDDDDDD
                     SSGSALEPSPTVTVEGGSVRGGTLKVALTGDPPNLDLMQTTDSIVMLVTSHFYETLFT
                     WDADYRAVPLLAASHEVSEDGLVNTVKLREDVPFHNGEAMQAADVIASVNRWGKLSGL
                     GESLLEATDEIVEVDPTTIEFRMNQPFGTFAMSLARQLQGCAIYPKSILDKSDESSLA
                     EYIGTGPYRFVEWEPDVRVRVERFDDYAPAEGEPSGYAGAKAQHLDAIEFIPVRNEAS
                     RIAGIQAGDYHYLETASPDQAGTLAGSANVAVDTLPADAWLNIVLNLRSVLLSDLNVR
                     RAVQTALDHAAIMQAAFGEGYYELTPELIPGAPTWYSEAGIEYFNRNDPDEARKLLDE
                     AGYDSTPLRIMTTQEVQQEYNGTLTMSQQLEAVGFKVDLQVYDGATLSDRRDDEVVWE
                     MYTAWASFRPDPVMRNLTASAAGWWEDDEKDQLLADLQSEADYDKRFAIWEQVQARFY
                     EDVPRLKIGNSLRILVRAASLQGIGPTEMQPEFSNAWLEG"
     gene            complement(8494..9252)
                     /gene="map"
                     /locus_tag="VFV93_16795"
     CDS             complement(8494..9252)
                     /gene="map"
                     /locus_tag="VFV93_16795"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX5167068.1"
                     /translation="MSRGKINLKNDKQIALMRDAGRLVAETHAMIQEHIRPGVTTAEL
                     DRITEAYIRLHGGVPSFKGFHGFPASICVAVNDQVVHGIPGRQTLEEGDVIALDIGII
                     LNGWHGDSCWTYAVGAIDSESRRLMDVCHDSLFIGIEQARAGKRLTDISFAIEQYVNA
                     NGFAVVRDLFGHGIGRNMHEAPSLPHYGPAGDGPVLKPGMVLTIEPMIVAGSPEVLTL
                     RDGWTIITKDGSRSAQYEHTIAITNNGPEILTLP"
     gene            complement(9398..11347)
                     /gene="acs"
                     /locus_tag="VFV93_16800"
     CDS             complement(9398..11347)
                     /gene="acs"
                     /locus_tag="VFV93_16800"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX5167069.1"
                     /translation="MTTTTTKAPNLDWETTSRVLSEDRLFQPSQEVVENANITAYMRD
                     KGFSTYDELHDWSVEHNEEFWAEMASELDWFKPWDQVLDWQPPYAKWFVGGETNIVYN
                     ALDRHMSTPTRNKVAFYWEGEDGTSRTISYSDLYYDVCRFASVLQNMGIEAGDRIVIY
                     MPRIPEQIVAMMAAARIGVVHSVVYSGFSAHALRDRVQDAEAKAVITTDGYFYRGKVV
                     GLKGIVDDAVRECPSVKRVITVKRAGNDINWHRGRDAWYHELMELTDGAAEVAHLDSE
                     APLYMLYTSGTTGKPKGIVHVHGGYMVGIYATMKFVFDMKPDDVYWCSADPGWVTGHS
                     YIVYGPLIAGATGIFYEGAIDYPDPGRIWRMIEKYGVTIFYTSPTAIRSLMRFGEGWP
                     GAADLSTLRLLGTVGEPINPEAWVWYRKIAGGDQPVIDTWWQTETGMQMITPTPITPL
                     KPGSATKEFLGVQASIVDRAGNAVGANEGGYLVIHKPWPSMMRTIFGDPERYEQYWNT
                     IPGVYFAGDAAHQDADGYFWVQGRVDDVIKKSGYRLGSSEIESALVSHPAVAEAAVIG
                     KPDDVKGEAIKAFVILKTGYDSSEQMLQTLRLHVRENVGPIAVPEEIEFVPSLPKTRS
                     GKIMRRVLKARELGQEVGDLSTLEE"
     gene            complement(11469..>11735)
                     /locus_tag="VFV93_16805"
     CDS             complement(11469..>11735)
                     /locus_tag="VFV93_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167070.1"
                     /translation="LLSALNPGNGLILMSCEPGWSGDWHPGPRRQWMLWLAGATTIEV
                     SDGETRTIAAGTILLSEDTTGKGHRSWDAGPERVLIAVVPIPDA"
BASE COUNT         2074 a         3641 c         4019 g         2001 t
ORIGIN      
        1 cgcggcgagt tcgacaacct ggccggccag ggcaagccgc aggacctgac gaccgaccac
       61 cacgccggcg accgcgagag tggcttccgc gtcctcaaga acaacgggcc tgcgccggcc
      121 tggatcgagg ccgacaaagc catccgggcc gaacaggcgg cactggccga actggcagct
      181 cgaacagcca gcttcatcac cgaacacctc gacgacgaaa cgacgaacgc cccagcgcca
      241 gccgcgccga cgttctcggc acgactgcgc cgcctctggt ccggcagccg gcgacccgcc
      301 caaccagtca accacttcac ccgagccgat ctgcgcaacg aacgcgaccg cgtacgccgg
      361 gagtatctgc gtcgctcgga cgaactcaac cagacgatca acgagtacaa cagcgccctg
      421 ccgttcgacc tcctctggcg cgagcgacag aagatcaccc ccgaacaggc ggcggcgacg
      481 ttcaacagcg cctgcccgcc ggtgggctga gtgctgagtg ccagagccgg atggcactca
      541 caagggccgc acacgctccc ctgtcatcct gagcggtcct cgctgacgcc gttcgcctgg
      601 atcgcatgcg cagtcacccg tcccacgtcc cgcatcccgc gtcccgcatt ccgcgtcgcg
      661 gtcagattct tgcgtcccgt gcggtagcgg ttcgcgttgc catcgctgga aggagcgagc
      721 aggcatgccg gatacatggg aaacgctcgc gcaggcgatc gaggagcagc ggcaggtgct
      781 ggccacctat aacggacgac cacgtgcgtt ctgtccccat gcgctgggga tgaagggcga
      841 caaacggcat gtgctggtct accagttcga tgcagccaga cgaatcggcg agcggccgac
      901 gaccggctgg cgctgtctgg aagtcgaccg gcttgaagac gtgagcttgc atatcggcga
      961 gtggcatacc gcgccgaacg tcttcaatcc gcagacatgc ctcgaccaga ttgacgttgt
     1021 cgtcagtccg tttcagcagc gacggcagcc ggcggcacgg tagctcgtgc ctattcctcg
     1081 tgttcgattg cttcagcgcg ggtaatcacg tccatatctt caatcgcggg cgctggttgc
     1141 gggacggcct gagtcgtgat gacggccgtc tcgctccggg ccgacgactt caggcgcttg
     1201 aacacccaca tctcgtcgga cgtcactccg ctggtggcgc tgcccggctc gctggagcgg
     1261 gtgatgaccg tcacgagctc ccagccttca tcgccgagcg ccggtagctc ggagacgatc
     1321 ggctcatccc agccgccgat gcggttgacg ccgacgccac gaacccgatg taagaccttg
     1381 tattcccact tgtgcatcgt cgttttcgac ccttctcgcg ctttccgatc ccgcgatcgg
     1441 tctcttgtcg cgcgccagca tgatactgaa aactggccca actttatctg cacaccagaa
     1501 ggggagtgac catcggcaaa ccaccctgtt gggtggggcg atgccggttt gcgaacgcct
     1561 atgctccctg cgacgtgcgg cctgggcgaa tggccgttgt cgtcgtgtgt ctcggtacat
     1621 cgagcgggga ggggaaacga tgggcgctgg aacggcggtc ctgccgcgca agccggtgct
     1681 ggcggagagc gacttcagcc gtcagccgtt cgtgctggcc tgggaactga cccgcgcctg
     1741 taacctggcg tgtgtccatt gcagagcaga ggcgctacta cggcgtgatc cgcgcgaact
     1801 ctcgacggca gaggcgctgg ctgtaatcga cgacatcgca gggttcgacg tgccgccggt
     1861 gctgatcctg accggcggtg acccgatgcg gcggccggat ctgaacgacc tgatccggca
     1921 tgcgactgag cgcaacgtac gctgcacgct gacaccggca ggaacggcgc tgacgagcga
     1981 gcggcggctg gccgaggcga aggaggcggg actttcgagg atcgccgtca gtctggatgg
     2041 cccgacggct gaggaacatg acagctttcg tcgggtcaaa ggatcgttcg gctggtcgct
     2101 ggacattctc agcgcagcgg agcgattgca gattccggtc cagatccaca cgacgctgtg
     2161 caagcggaca ttgcacctga tgccggcgat ggccgatttg gcagaacagc tcggagccgt
     2221 cgtctgggcc atcttctgcc tcgtgccgac tggccgcggt gtcgatctgg acgaactgac
     2281 tgccgacgag tacgagatga tcttcgagtg gctggtcgaa cgcagcgcga cggcgtcgtg
     2341 gaatctgaag ctgaccgagg ggtatcacta ccgccgtgtg ctgatccagc accggcaggc
     2401 gccggtcgct ggtccgggct ttcaatcgag cgatgggatc ggcagggcga cacgggcggt
     2461 caacgccggc aacgggttct gcttcatctc gcatactggg gatatctgtc cgagtggctt
     2521 tctgccagtt gtcgccggta atgtccgcaa cgactcggtc gttgacgtgt atcgcgatca
     2581 cagagtcttc cgcgagttgc gcgatccgtc actgctggcc ggcaagtgtg ggcactgcga
     2641 gtttcgcgac atctgtggcg gctcgcggtc acgtgccttc gcccggacgg gagactacct
     2701 cgagagcgac acggcctgcg cccacatgcc gggtgacggc gcagcactgt cattcaggaa
     2761 ctgacacgtc cgccacgttg cgcgcgtagg ggtatgcggt tagcatccgc cgcagtactg
     2821 gtcagtggat cgagcgtccg acccattctc gccaactcgc gcgcgtaatc tgaggagggt
     2881 gtcatggtcc agacacaggt tcgcaacgtc ctcgaacctc tttcggcaga agaggttcgg
     2941 gcagcagtcc ggatactgaa gtcagaaacg acgttaccgg agaaacaccg cttcatccag
     3001 gttgcgctgc gcgagccgga caaggaacaa gcgctggcgg cgctggccgg ctcgaatggg
     3061 cacctgccgg atcgcgaggc ggcggtcgtc ctgctggacc acgaccggcg ttcgacccgg
     3121 gagggcgtcg tctcgatctc agccggaacg gtcgtcagct ggaacgaact gcccgatgtc
     3181 cagtcgccga ttgcgatgga agagttcttc gagtgcgaga aagcctgcaa ggctgacccc
     3241 gccttccgcg aggcgctggc gaagcgcggc gtgaccgaca tggacctggt gatggtcgat
     3301 ccctggtcgg cagggcacta cgaggacaac gagggccgtc ggctgtcgcg ggcgctggtc
     3361 tgggtacggg cagatccgga ggacaacggc tacgcgcacc cggttgagaa cctgatcgtg
     3421 ctggtcgacc tgcacgagat ggcggtctac cggatcgagg atttcggcgt cgtcccgatt
     3481 ccttgggcgg acggcaactt cgcgccgaaa tacaccggcg agccgaggac ggacctgaag
     3541 ccgatcgaga tcacccagcc ggaaggggtc tcgttcaccg tcgaaggaca cgaggtcgcc
     3601 tggcagaact ggcgctttcg ggtcgggttc acgccgcgcg aggggttggt gctgcacaca
     3661 gtcggttggg atgacggcga cagcctgcgg ccgattctct atcgcgcgtc gctggcggag
     3721 atggtcgtcc cgtacggcga tacgaacatc acccaggcgc gcaagaacgc gtttgacgtt
     3781 ggcgagtaca acatcggcct gctggcgaac tcgctggagc tcggttgcga ttgcctcggc
     3841 gagatctact acttcgacgc cgttgtctgc gacaaccggg gcgaaccgat gacgatcacc
     3901 aatgcgatct gcatgcacga agaggacgcc agcctgctct ggaagcattc cgactttcgg
     3961 attggaacca gcgaggtgcg ccgctcgcgc aagctggtcg tctcgttcat ctcgacggtc
     4021 ggcaactacg actacggctt ctactggagt ttcttccagg acggcacgat ccacttcgag
     4081 atcaagcaga cgggaatcgt gacgacggcc gtgacggaga gcagcgagcc gctgaagtat
     4141 gggcagttgc tcaacaccgg cgggctctac gcgccgattc accagcactt ctacaacatc
     4201 cggctcgata tggacgtcga tgggaccgac aactcgctct acgaggtcca cgccgagcgt
     4261 gtcccggctg gagccgacaa cccgtacaac aacgcgttct actcgaagtc gacgttgctg
     4321 cggcgtgagt cggaagcgca gcaggacatc gatccaatga ccggacgggt ctggaagatc
     4381 gtctccaaca gccggacgaa cgctgtcggc gagccaaccg cctaccggct ggtgccgcac
     4441 accaacgtgg cagccttcgc ggcccccgaa gccagcgtca cgaaacgggc cggattcatc
     4501 acgaagcatc tctgggcaac gccgtggcag cgcaatgaga acttcccggc cggcgactac
     4561 ccgaatcagc atcccggcgg cgccggcctg cccgagtgga cgtcggccga ccgcgagatc
     4621 gagaataccg atctggtgct ctggtacacc ctcggagccc accacccgcc tcgcctcgaa
     4681 gactggccga ttatgccggt gaccggcgcc agcttcgtgc tgcaaccagc cggcttcttc
     4741 gaccgcaacc cgacgctgga tgtcgcgccg cccaaggggc attgtcatcc gtgatgggtg
     4801 atgcgttcca ggcggccagg tcctcgtaaa cgtcggccgc ctggaacgca tcacgctcta
     4861 ccgccgtggg tccagcgcat cgcgcaacct atcgccgatg acggtcaggg cgaggacggt
     4921 caggaagagc agcgtgccgg gggcgacggc gatccaccag gcggggccga gcaggcgctg
     4981 gccgtcgcgg agcatgttgc cccagctggg ggcttgtggg tcaccgccgg caccgaggaa
     5041 ggcgagcgag gcttcggcga ggatcgcgta ggcggcgatg aaggtgcatt gcacgatcag
     5101 cggcgagagt gaattggcga gtacgtagcg ccagaggatg cgcaggtcgg tctggccgat
     5161 ggcgcgggcg gattcgatgt agggtagttc tcggatcagc agtgtcgtcg agcgcatcag
     5221 ccgggcgatg gtcgggatgt agaccagcga gagagccagg atgacgctgc tggtccgtgc
     5281 gccgaagctg atgacgacgg cgatcgccag caggacgccg gggaaggcca tgaagccgtc
     5341 catgacgcgc atgatcagcg ggtcgaggcg gcggtagtaa ccggagacaa caccaaagaa
     5401 cgttccgagc gcggcgctga cggccgtgac gactgcgccg accagcaacg agacgcgacc
     5461 accgtcgatc aggcggctga agacatcgcg gccaagctcg tcggtgccga gcaggtggtc
     5521 ggccgatggc ggctggaggc gaatggccgg ggcgatcagg cgcgggtcgt ggggaacgat
     5581 cagcggcgag aggactgccg ccagcgccag cacgacgatg aaggcgaggc tcgccagtgc
     5641 cagcggagag gcaacgatcc gccgtgtccc gagggacggg ccggggcgca gcgggcggac
     5701 ggtgacgttg ccggcactgg tgatgctggc cttaccggct gacatggccg tgccggatgc
     5761 gtggatcgat gaccatgtag agcacgtcaa ccagcaggtt ggcgagcagg tagcagccgg
     5821 cgattgccat cacgacaccc tggataacgg ggaagtcgcg gcgggcgatg ctgttgacga
     5881 ccagttggcc catgcccggc aggttgaaga cggtctcaac gacgacggcg ccgccgagca
     5941 ggtcgccaag cgtgtagccg aggacagtca cggccggcaa cagcgcattg cgaagggcgt
     6001 ggcgcacgac gacagtggcc ggcgagagac cacgggcgat cgcggcgcgg acgtagtctt
     6061 cctgaaggac atccagcagc gaggcgcgga tcagccgggc cggcaaaccc gcgatcgaga
     6121 gtccgagcgc gaaggccggc agcaccatcg agcgggcgtg gtcaatcgga gagtccaaca
     6181 acgggacgta gccacccgaa ggtagccaac gaagggtcac tgcaaagagc aggacgagca
     6241 ggatcgcgag gaagaagtcg gcgatcgccg tcccggcgac ggccagcagc atcagcagcc
     6301 ggtcgatcag cgagttctgg cgaacggcgg cgatgacgcc ggccggaacg ccgatcacca
     6361 gcgcgatgac catcgcgtag agcgtgagtt gcagggtcgg gacaatccgg ccagcgatcg
     6421 cggtcgcgac gggccggtcg aggaagagcg agtcgccaaa gtcgagccgg aggatgccca
     6481 gcagccagcg cgggtattgc actagcagcg gatcgtccag cccgagccgt tcacgggcgc
     6541 gggcgacctg ttcgggcgtc gcctcggagc cgagcatgac ggcggccgga tcgccgggcg
     6601 cgacgtgcag cagcgtgaag gtgatcgtgc cgacgatcag cgccaccgga accagcgcca
     6661 gtagccgccg gacgacgagc ccgatcatcc ctccagccag gcgttcgaga actccggctg
     6721 catctcggtc gggccgatgc cttgcaggct ggcggcccgg acgaggatcc ggaggctgtt
     6781 gccgatcttg agacggggca catcctcgta gaaacgagcc tggacctgct cccagatcgc
     6841 gaagcgcttg tcgtaatccg cttcggattg gagatcggcg agcaactggt ccttctcgtc
     6901 gtcctcccac caaccggcag ccgaggcggt caggttgcgc atgacagggt cgggccggaa
     6961 gctggcccag gcggtgtaca tctcccagac aacctcgtcg tcacgccggt cgctcagcgt
     7021 ggcgccgtcg taaacctgga ggtcgacctt gaagccgact gcctcaagct gctgggacat
     7081 cgtcagcgtg ccgttgtact cctgctggac ttcctgggtc gtcatgatgc gcagcggggt
     7141 gctgtcgtag ccggcctcgt cgagcagctt ccgggcttcg tcgggatcgt tgcggttgaa
     7201 gtactcgatg ccggcctcgc tgtaccaggt cggcgcgccg gggatgagct ccggcgtcag
     7261 ttcgtagtat ccctcgccga aggcggcctg catgatggcg gcgtggtcca gcgccgtctg
     7321 gaccgcccgg cgaacgttga gatcgctcag tagtaccgag cgcaggttga ggacgatatt
     7381 cagccaggcg tcggccggca gtgtgtcgac ggcgacgttg gcgctgcctg ccagcgtgcc
     7441 agcctggtcg gggctggctg tttcgaggta gtggtagtcg cccgcctgaa tgccggcgat
     7501 ccgcgacgcc tcgttgcgga ccgggatgaa ctcgatcgcg tcaaggtgtt gggctttcgc
     7561 cccggcgtag ccactcggtt cgccctcggc cggcgcgtag tcatcgaagc gctcgactcg
     7621 gacgcggaca tccggctccc actcgacgaa gcggtacggg ccggtgccga tgtattcggc
     7681 aagactcgac tcgtcggact tgtcgaggat cgatttcggg tagatcgcgc agccctggag
     7741 ctggcgggcc agcgacattg cgaaggtgcc gaacggttgg ttcatccgga actcgatcgt
     7801 cgtcggatcc acctcgacga tctcgtcggt cgcctccagc aggctctcgc ccaatccgga
     7861 gagcttgccc cagcggttga ccgaggcaat cacgtcggcg gcctgcatcg cctcgccgtt
     7921 gtggaacggg acatcctcac gcagcttcac cgtgttgacg agtccatctt cgctgacctc
     7981 gtggctggcg gccagcagcg ggaccgcgcg gtagtcggcg tcccaggtaa agagcgtctc
     8041 gtagaagtgt gacgttacca gcatgacgat cgaatcggtc gtctgcatca ggtcgaggtt
     8101 cggaggatca ccggtcagcg cgaccttcaa cgtaccgccg cgaacggaac cgccctcgac
     8161 ggtcacggtc gggctcggtt ctagcgcgga ccccgacgag tcgtcatcgt catcgtcgcc
     8221 cccgcaggcg gcgagtaacc cggtggccgg gattgccagt ccaataccag cggcggcccg
     8281 caagagcgtc cggcggctca gccgtcgatg cagcaagtcg tcgcgaacca acgtgaatcc
     8341 tcatctcgtt cgtttcatgc ttctcatctg cccggaagca gcgaacgtca attgtcacgg
     8401 acggagcggg gtaggtcaaa ttgggatgac gcaggacgcg taatgcgtga cgcgtgacgt
     8461 ggcagagggc gacgccgcgt catgcgtcac gcgtcacggc aacgtcaaaa tctccgggcc
     8521 gttgttcgta atcgcgatgg tgtgctcgta ttgggcggag cgggagccgt ctttggtgat
     8581 gatcgtccag ccgtcgcgga gggtgaggac ctcgggagag ccggcgacga tcatcggctc
     8641 gatcgtcagg accatgccgg gcttgaggac cggaccgtcg ccggccggac cgtagtgggg
     8701 tagcgacggg gcctcgtgca tgttgcggcc gatgccatgg ccgaagaggt cgcgaacgac
     8761 ggcgaagccg ttggcgttga catactgctc gatcgcgaag gagatgtcgg tcagccgctt
     8821 gccggccctg gcctgctcga tgccgatgaa gagcgagtca tggcagacat ccatcaggcg
     8881 tcgcgactcg ctgtcgatcg cgccaacggc gtaggtccag caggaatcgc cgtgccagcc
     8941 gttgaggatg atgccgatgt cgagcgcgat cacgtcgcct tcttcgaggg tctgccggcc
     9001 gggaatgccg tggacgacct ggtcgttgac ggcaacgcag atgctggccg ggaagccgtg
     9061 aaagcctttg aaggacggca cgccgccgtg cagccggatg taggcctcgg tgatccggtc
     9121 gagctcggca gtcgtgacgc cggggcggat gtgctcctgg atcatcgcgt gcgtctcggc
     9181 gacgaggcgg ccggcgtcgc gcatgagggc gatctgcttg tcgttcttga gattgatctt
     9241 gcctcgggac atggttgagc ggtcagcttt cagctagcag ccgtcagcgt gacacggatg
     9301 acggctcggg atgaatggca tgggggcgtc acagatacgc ccccatcgaa acgcgactca
     9361 attgtaccgc cagcgggcaa tctcgcccgg ctgactccta ctcctccagc gtgctcaggt
     9421 cgccaacctc ctggcccagc tcgcgggcct tgaggacgcg gcgcatgatc ttgccggagc
     9481 gggtcttcgg cagcgacggg acgaactcga tctcttcggg gacagcgatc gggccgacgt
     9541 tctcgcggac atgcagccgg agcgtctgga gcatctgctc gctggagtcg tagccagtct
     9601 tgaggatgac gaaggccttg atcgcctcgc ccttaacgtc atcgggcttg ccgatgacgg
     9661 ccgcctcggc gacagcgggg tgcgaaacga gcgcggactc gatctccgac gagccgagcc
     9721 ggtagccgga cttcttgatg acgtcgtcga cgcggccctg gacccagaag tagccgtcgg
     9781 catcctggtg ggcggcatca ccggcgaagt agacgccggg gatcgtgttc cagtactgct
     9841 cgtagcgttc cgggtcgccg aagatggtgc gcatcatgct tggccacggc ttgtggatga
     9901 cgaggtagcc gccctcgtta gcgccgacgg cgttgccggc ccggtcaacg atgctggcct
     9961 ggacgccgag gaactccttg gtggcagatc ccggcttgag cggcgtgatc ggcgtcggtg
    10021 tgatcatctg catgccggtc tcggtctgcc accaggtgtc gatcaccggc tggtcgcctc
    10081 cggcgatctt gcggtaccag acccaggcct cggggttgat cggctcaccg accgtgccga
    10141 gtagccgcag cgtcgagagg tcggcggcgc caggccagcc ctcgccgaag cgcatcagcg
    10201 agcggatggc ggtcggcgac gtgtagaaga tggtgacgcc gtacttctcg atcatcctcc
    10261 agatccggcc cggatcgggg tagtcgattg ccccctcgta gaagatgccg gtcgcgccgg
    10321 cgatcagcgg gccgtagacg atgtatgagt ggccggtgac ccagccggga tcggccgagc
    10381 accagtagac gtcgtccggc ttcatgtcga agacgaactt catcgtggcg tagatgccga
    10441 ccatgtagcc gccgtggacg tggacgatgc cctttggctt gccggtcgtt cccgaggtat
    10501 agagcatgta gagcggcgct tccgagtcga ggtgggcaac ctcggcggct ccgtcggtca
    10561 gctccatcag ctcgtggtac caggcgtcgc gtccacggtg ccagttgatg tcgttgccgg
    10621 cccgcttgac ggtgatgacg cgcttgacgc tcgggcactc gcgaactgcg tcgtcgacga
    10681 tgcccttcag gccaacgacc ttgccgcgat agaagtagcc atcggtggtg atgacggcct
    10741 tggcctcggc gtcctgaacg cggtcacgca gcgcatgggc cgagaagccc gagtagacga
    10801 ccgagtggac gacgccgatc cgggcagccg ccatcatcgc gacgatctgc tctgggatgc
    10861 gcggcatgta gatgacgatc cggtcgccgg cctcgatgcc catgttctgg aggacgctcg
    10921 cgaagcggca gacatcgtag tagaggtcgc tgtaggagat ggttcggctg gtgccgtcct
    10981 cgccctccca gtagaaggcg accttgttgc gggttggcgt ggacatgtgg cggtcgaggg
    11041 cgttgtagac gatgttggtc tcaccgccga cgaaccactt ggcgtagggt ggctgccagt
    11101 caaggacctg gtcccacggc ttgaaccagt ccagctcgga cgccatctcg gcccagaact
    11161 cttcgttgtg ctcgaccgac cagtcgtgca gctcgtcgta ggtcgagaag cccttgtcgc
    11221 gcatgtaagc ggtgatgttg gcgttctcga cgacctcctg gctgggttgg aagagccggt
    11281 cctcgctgag gacgcgactg gtcgtttccc agtcaagatt cggcgccttg gtggtcgtgg
    11341 tcgtcatccg cccctcccct gttcactgtt gcgggtattg cctctgcaca cgcctgacgg
    11401 cgcaagcggt tgtgcattgc gtcgctggtc catcgattct agcaccggtt tcgcgattgg
    11461 cgcgcggatc aggcgtccgg gatcggcacg accgcgatca aaacgcgttc cgggccggcg
    11521 tcccaggagc ggtggccctt gccggtcgtg tcctcggaga gcaggatcgt gccggccgcg
    11581 atcgttcggg tttcgccgtc gctgacctcg atcgtggttg cgccggccag ccagagcatc
    11641 cactggcgac gcgggccggg gtgccagtcg ccggaccagc ccggttcaca gctcatgagg
    11701 atcaggccgt taccagggtt gagcgccgag agcag
//
LOCUS       DASUGJ010000743         5911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_263258, whole genome shotgun sequence.
ACCESSION   DASUGJ010000743 DASUGJ010000000
VERSION     DASUGJ010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263258"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="VFV93_16810"
     CDS             <1..1473
                     /locus_tag="VFV93_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167071.1"
                     /translation="ALAFLEHAARNRRQWLRNYAGVMRRTVEPQADEKPYAFLIPAGQ
                     DDPGTAFELLETLSLGDVEIEETTAGFSASGIDWPAGTRVIRLAQPAGKYVKTLLELQ
                     EYPNLRRWPDGPPSPPYDIAGHTLPLQMGVRAVQVDEPFEAELTRTEIQRPAGSVEGA
                     GRFGWAISSRTNWSLLAANRLQKGGFTIQRLTAEHGQLPAGTFVLPAQDGLDEAIRSV
                     ASDVGVDVIGIDTELEAEAIEFGAARIGVYQSWRPTIDEGWVRWIFEEYELPYTTLHD
                     NDIRQGNLRERFDTLLLPQQKAEDILKGNPEKNQYREPFPPEYVGGLGQLGVDALREF
                     VESGGTLVAIDSATEFAIKQLYLPVRNVVEGLSEEEFYCPGSLLRLIVDSQHPLGYGL
                     RRNEVALFMKSPAFELGSGSNGSVVASYPFYEPNLSGWILGSDKLAGKGALVEVSLGA
                     GRVVLIGFRAQFRAQARGTYKVLFNAVLRTGRQPELLRLG"
     gene            complement(1500..1970)
                     /locus_tag="VFV93_16815"
     CDS             complement(1500..1970)
                     /locus_tag="VFV93_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167072.1"
                     /translation="MSIDSGRLTEGMTVVGPRGEQIGRVKDVRTADFLMDMSMRRDLF
                     VPFSAISEIGGSVTLNVRREDIDEQGWEPSPLLGAPAASSNESPSETPPREDRVVGTG
                     DPAERGTWSASSDSYDLDAIDTTEGRNAIEGTDDGERPLPGDTTEMDKLRDLSG"
     gene            complement(2309..2683)
                     /locus_tag="VFV93_16820"
     CDS             complement(2309..2683)
                     /locus_tag="VFV93_16820"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5167073.1"
                     /translation="MTQREVLQSDKLPVPAAPYSVATIWGDLLFISGQVPADTSQDIA
                     GQTEQVMEKLKAIMTEAGTGFEHALKATCFLTDISEFATFNEVYSRYVGDAKPARSTI
                     ECKLAGPAFKVEVDMVVGIPNK"
     gene            2827..3168
                     /locus_tag="VFV93_16825"
     CDS             2827..3168
                     /locus_tag="VFV93_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX5167074.1"
                     /translation="MSVKLVPEPGENVPAVDADQLSTGASYEHDKAKILARLRRMEGQ
                     VRGVQRMVEEDKYCVDVLTQISSIIAAARAVGLLVLEDHIRGCVVNATDDEVMVTELT
                     DAIERFTRSVG"
     gene            complement(3179..4531)
                     /locus_tag="VFV93_16830"
     CDS             complement(3179..4531)
                     /locus_tag="VFV93_16830"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX5167075.1"
                     /translation="MAAVDEGALNSLQQRTEADDLDGLWITFHDYSARACAKWIPRAS
                     IPAALRRGGVFARANLNFTIDDHQVPQPHFAADSGDMFAVPDPDTYAPIPYRPGFGRV
                     LSYLCTEEGDLWEGCPRGRLQAALDALAERGFSAQAAFEPEFTFYERRADDGYQPVDH
                     FAMYSVDRIDANASLLGRIDKALTAQGVRVIQLGTEYGAGQLEINLHHETPMKAADDL
                     VTFRETVKALARDAGIVASFMPKPFADAAGNGVHVHLSLWDAEGQTSRSEGEGPLGLS
                     PDLAHFMAGVLAHAPALCGVAAPTVNSYKRLLPGSWAPGHIAWGSGNRAVLVRVPGSS
                     RRRIEFRAGDHTGNPHLFLAALLAAGIDGLDRQLDPGAPVEGDIGHMSIADVERMGGR
                     FLPRTAHDALNAIEADETVMAALGPVCGPELLRIKRYELERYDTQVSDWEREVYLERA
                     "
     gene            4663..5841
                     /locus_tag="VFV93_16835"
     CDS             4663..5841
                     /locus_tag="VFV93_16835"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5167076.1"
                     /translation="MIDLSGLPVVDIHCHPFLNRGPLPADEFTNLASFGGGSVAYMEQ
                     GGIEVDDAVLAELQRVKRDTVYFRRLVRDLAAFFGTEPTLDAVLVARNRAVSDDYRGY
                     VQRLYADVGLETLVFDFGYPVPNLPVEPVRAELPVEVVPILRIEPLIVDLLAADIGWA
                     EFKRRYDDTIAEALSTGGYRGLKSIIAYRTGLDVSPLSRSPDQGMQALDAIRRGLGGG
                     SMKKLRDHLLCRALELAMEHDVPMQIHTGMGDFEVNLPLARPSLLLDLLRFPTFRACR
                     VILVHTGYPWHREAGYIANVLPRVYCDVSEGIPFAGHAARSIISEVLEMAPLSKVVYG
                     SDGFNVPEINYSSAKLGKQALAQVLTGLVDDRMLTESDAQEAGAMILSGTARRLYGLQ
                     "
BASE COUNT          959 a         1911 c         1976 g         1065 t
ORIGIN      
        1 gccctcgcgt ttcttgagca tgccgcccgc aaccgccgcc agtggctgcg caactatgcc
       61 ggcgtcatgc gccgtaccgt cgagccgcag gcggatgaga agccatatgc cttcctgatc
      121 ccggccggac aggacgatcc tggtacggcc ttcgagctgc tggagacgct gtcgctcggc
      181 gatgttgaga ttgaagagac aaccgctgga ttcagcgcca gcggcatcga ctggccggcc
      241 gggacgcggg tgatcagact ggcgcaaccg gccgggaaat atgtcaagac gctgctggag
      301 cttcaggagt atcccaacct gcgccgttgg ccggatggcc cgccatcccc accgtacgac
      361 atcgcgggcc acacgctgcc gttgcagatg ggcgttcgcg ccgttcaggt cgatgagccg
      421 ttcgaggccg aactgacccg cactgaaatt cagcgcccgg ccgggagtgt ggagggcgcg
      481 gggcggttcg ggtgggcgat ctcctcgcgc acgaactggt cgctgctcgc tgccaaccgg
      541 ctgcagaaag gtggcttcac gatccagcgg ctgactgcgg agcacggcca gttgccggcc
      601 ggaacgttcg tgctgccggc tcaggatgga ctcgacgagg cgatccggag cgtcgcaagc
      661 gacgtaggcg tcgatgtcat cgggatcgat accgaacttg aggcggaggc aatcgagttc
      721 ggggcagcac ggattggcgt ctaccagtcg tggcggccga cgatcgacga gggctgggtg
      781 cgctggatct tcgaggagta cgagctaccc tacacgacgc tccacgacaa cgacattcgc
      841 cagggcaacc tgcgcgagcg cttcgacacg ctcctgctgc cgcagcagaa ggctgaggac
      901 atcctcaagg gcaatccgga gaagaatcag tacagggagc cgttcccgcc ggaatacgtc
      961 ggcggtctcg gccagttggg cgtcgatgcg ctgcgcgagt tcgtcgaatc cggcgggacg
     1021 ctggtggcga tcgattcggc gaccgagttc gcgatcaagc agctgtatct gccggtgcgc
     1081 aacgtcgtcg aggggctgtc cgaggaggaa ttctactgtc ccggctcgct gttgcggctg
     1141 atcgtcgatt cccagcaccc gctcggctac ggcttgcgac gcaacgaggt cgcgctgttc
     1201 atgaagagtc cggcatttga gcttggttcc ggttcgaacg gcagcgtcgt cgcctcatac
     1261 ccattctacg agccgaacct gagcggctgg atcctcggca gcgacaagct ggccggcaaa
     1321 ggcgcgctgg tcgaggtgtc gctcggcgcg ggccgtgtcg tgctgatcgg cttccgggcg
     1381 cagttccgcg ctcaggcgcg cggcacctat aaggtgctct tcaacgcggt cctgcgaacc
     1441 ggaaggcagc cggaactgct gcgactcggc taatccgttg tcgggcggcg cggaccgcgc
     1501 tagccggaca agtcgcgcag cttgtccatc tcggtcgtgt cgcccggtag cggccgctca
     1561 ccgtcgtcgg tgccttcgat cgcgttgcgg ccctcggtcg tgtcgatcgc gtcgaggtcg
     1621 tacgagtcgg acgacgcgct ccacgtcccc cgctcggccg gatcgccggt tccgacgacg
     1681 cggtcttctc ggggcggtgt ctcgctgggt gactcgttag aggacgcagc cggggcgccc
     1741 agcagcggcg agggttccca gccctgctcg tcgatgtctt cccggcgaac gttgagggtg
     1801 acgctgccgc cgatctcgct gatggcgctg aacggcacga acagatcgcg acgcatcgac
     1861 atatccatca ggaagtcggc cgtccgtacg tccttgacgc ggccgatctg ctcgccccgc
     1921 ggcccgacga cggtcatccc ctcggtcagc cgccctgaat caatgctcat cgtactgtcc
     1981 cttcacctgt gaacgatctg cccactgcgg gccatgcaag cgacatgccg gtggtaatgg
     2041 gacagcagga tgcgaactct ttgtgcaacc agccgaacgg cttaatcggg gatcagcacc
     2101 cctggccctg tcatcctgag ccgcagcgaa ggatcgctca acgtctgacg ctggagcgcc
     2161 ccgatcggtc gagcgcgctc ggcgttttcg aaccggtcct gtcgtcagcg ccaggcgatc
     2221 cttcgctgcg gctcaggatg acagggcaag gggtgctgat ccccgataaa gccgttcggc
     2281 gggggagtgg cgtctggccg cttttcagct acttattcgg aatgcccacg accatgtcga
     2341 cctcgacttt gaaggctggg ccggcgagct tgcattcgat cgtcgagcgg gccggcttgg
     2401 catcgccgac gtagcggctg tagacctcgt tgaaggtggc gaactcgctg atgtcggtca
     2461 ggaagcaggt cgccttgagc gcgtgctcga aaccggttcc agcctcggtc atgatcgcct
     2521 tgagcttctc catcacctgc tcggtctgcc cggcgatgtc ctgcgaggtg tcggccggca
     2581 cctggccgga gatgaagagc agatcgcccc agatggtcgc caccgaatac ggcgcagccg
     2641 gcaccggcaa cttgtcggac tgaagtacct cgcgctgtgt catcgtcggg ttccttccat
     2701 ctcgtcagac tgattcgccg ggcgatgata cggccattcc cccgtccggg ccagcccgag
     2761 gcctgccgtt ggttgccgaa gataccccct tggggtatcc ttctgacaag cgaaaggatg
     2821 acgacgatgt cagtcaagct cgtacctgaa cctggtgaga acgttccagc cgtcgatgcc
     2881 gaccagctgt caacgggtgc gtcctacgaa cacgacaagg ccaagatcct cgcccgcctg
     2941 cgccggatgg agggtcaggt gcgcggcgtc cagcgcatgg tcgaagagga taagtactgc
     3001 gtcgatgtcc tgacgcagat ctcgtcgatc atcgccgccg cccgcgccgt cggcctgctc
     3061 gtcctcgaag accacatccg cggctgcgtc gtcaacgcca ccgacgacga agtgatggtc
     3121 accgagctca ccgacgcgat cgagcgcttc acccgcagtg ttggatagtg cctgtcacct
     3181 acgctcgctc cagatacacc tcgcgctccc agtcactgac ctgggtgtcg tagcgttcca
     3241 gttcgtagcg tttgatgcgc agcagttccg ggccgcagac cgggccgagg gcggccatga
     3301 cggtctcgtc ggcctcgata gcgttcaacg catcgtgggc ggtgcgaggc aggaacctgc
     3361 cacccatccg ctcgacatcg gcgatcgaca tgtggccgat gtcgccctcg accggcgcgc
     3421 ccgggtcgag ctggcggtcg agtccgtcaa tgccggccgc gagcagtgcg gccaggaaca
     3481 ggtgcgggtt gccggtgtgg tcgccggccc ggaactcgat ccggcggcgc gaggagccgg
     3541 gcacgcgtac cagtactgcc cggttgccgc tgccccaggc gatgtggccc ggcgcccacg
     3601 agccgggtag cagccgcttg tacgagttga ccgtcggcgc cgcgaccccg cagagggctg
     3661 gggcatgggc gagtacgccg gccatgaagt gcgccaggtc tggtgagagc ccgagcggcc
     3721 cttccccttc ggatcgactg gtctgcccct ccgcgtccca gagcgagaga tggacgtgga
     3781 cgccgttccc ggcggcatcg gcgaagggct tcggcatgaa gctggccaca atgccggcgt
     3841 cacgggccag tgccttgacc gtctcgcgaa aggtcaccag gtcgtcggcg gctttcatcg
     3901 gcgtctcatg gtgcaggttg atctcgagct ggccggcgcc atattcggtg ccgagctgaa
     3961 tcacgcggac accctgcgcc gtcagcgcct tgtcgatcct gccgagcagg ctggcgttgg
     4021 cgtcgatccg gtcgaccgag tacatggcga agtgatcgac cggctggtag ccatcgtcgg
     4081 cacgtcgctc gtagaaggtg aactccggct cgaaggcggc ctgggcgctg aagccgcgtt
     4141 cggcgagcgc gtccagcgcg gcctgcagtc gcccgcgcgg acaaccttcc cagagatcgc
     4201 cctcctcggt acagaggtag ctgagcaccc ggccgaagcc gggccggtag ggaatcggcg
     4261 cgtaggtgtc ggggtcgggg accgcgaaca tgtcgccgga gtcggccgcg aaatgcggct
     4321 gcgggacctg gtggtcatcg atcgtgaagt tcagattggc gcgagcgaag acgccgccac
     4381 gtcgcagcgc ggccgggatg ctggctcgtg gaatccactt ggcacaggcg cgcgcggagt
     4441 agtcgtggaa cgttatccag agcccgtcga gatcatcggc ctcggttcgc tgctgcagcg
     4501 agtttaacgc gccctcgtcg acggctgcca tgccactccc tcccacttgc aatcaccgga
     4561 cggatcatcg tcggggacaa taacataccg ccggtcgtgc gccagcgtcg gaatccggcg
     4621 ataatcgtcc gggtattcgt gtccacgaag ggagtcgctc gcgtgatcga tctgtcgggg
     4681 ttgccggtcg tggacatcca ctgccatccg ttcctcaacc gtggtccgtt gccggccgac
     4741 gagttcacca atctggcgtc gttcggtggt ggctcggtcg cctacatgga gcagggtggg
     4801 atcgaggtcg atgacgcggt gctggccgag ttgcagcgcg tgaagcgcga caccgtctac
     4861 ttccggcggc tggtgcgcga cctggctgcc ttcttcggca cggagccgac gctggatgcc
     4921 gtcctcgttg cccgtaatcg agcagtcagc gacgattatc gcggctatgt gcagcgactc
     4981 tatgccgacg tcggactgga aacccttgtc ttcgacttcg gctacccggt cccgaacctg
     5041 ccggtcgagc ctgttcgtgc cgaactgccg gttgaggtcg tcccgatcct gcggatcgag
     5101 ccgctgatcg tcgatttgct ggcagccgac atcggctggg ccgagttcaa gcgtcgctac
     5161 gacgacacga tcgccgaggc gctgtcgacc ggcggctatc gaggcttgaa gtcgatcatc
     5221 gcctaccgga ccggccttga tgtctcgccg ctctctcggt cgcccgatca agggatgcag
     5281 gctctggacg cgatccggcg cgggctgggc ggcggcagca tgaagaagct gcgcgaccat
     5341 ctcctttgcc gggcattgga actggcgatg gagcacgacg ttccgatgca gatccacacc
     5401 gggatggggg acttcgaggt caacctgccg ctggcccggc cgtcactgct gctcgatctg
     5461 ctgcgcttcc cgaccttccg tgcctgccgg gtgatactgg tccacaccgg ctacccgtgg
     5521 caccgcgagg ccggctacat cgccaacgtc ctgccgcgcg tctactgcga tgtctctgag
     5581 ggcatcccgt tcgccggcca cgccgcccgc tcgatcatca gcgaggtgct ggagatggcg
     5641 ccgctctcca aggtcgtcta cggttccgac gggttcaatg tgccggagat caactacagc
     5701 agcgccaagc tcggcaagca ggcgctggcg caggtcctga ccggcctcgt tgacgacaga
     5761 atgctgaccg aatccgatgc ccaggaggcc ggcgcgatga tcctgtccgg cactgcccgc
     5821 cggctgtatg ggttgcagta aacccccgtc gtcctgagcc gcagcgaagg acctctctgc
     5881 gctaacgccg tgctgggtca cagcggagag g
//
LOCUS       DASUGJ010000744         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_957510, whole genome shotgun sequence.
ACCESSION   DASUGJ010000744 DASUGJ010000000
VERSION     DASUGJ010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957510"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1078)
                     /locus_tag="VFV93_16840"
     CDS             complement(56..1078)
                     /locus_tag="VFV93_16840"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX5167077.1"
                     /translation="MSANQTHRVSSEHIHTYWSNAVPPVITVQPGDTVVFETLEASYG
                     HVCRNVRELAQPGLDPELIEIIAAAAYPARSHPERDPSITGGHALTGPVAIEGAEPGD
                     TLVVEIIDIAPAQWGYNACSPRAERLGLLNDDLGDLDHDMHWYWDLRNGDYADFKPGI
                     RVPAGKHPGVMGVAQAEPGQLSTIPPRTNGGNMDIRHLQPGATLYLPVLAPGALFSTG
                     DVHFAMGDGEVTGTGIEINSDVTLRFDLQKGATIPGPRLRTNGQPLSIPGAAFATTGF
                     DPDVREAARIALRAMLDHMQREYDLTRPEAYMLASVVVDLKISQIVDRPNWTVTAYLP
                     LSIFGE"
     gene            complement(1552..2502)
                     /locus_tag="VFV93_16845"
     CDS             complement(1552..2502)
                     /locus_tag="VFV93_16845"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX5167078.1"
                     /translation="MPDSPTILVHSGVAHENAIVAARIAEAQPGLTILTAHGEAQVRE
                     RLPQAEILFAWEFPMALLPQAERLRWFQVMGAGLERLAGASVPDGVIVTNMKGIFSTA
                     MAEYALAHMLAHTQRIQQILRQQQERRWEPFEPVLLRGQTAGIIGLGSIGREIARRCA
                     AFGMRVIGVNRGGAPVAEVERTYAVADIDRFLPECDFVISVVPHTAESTNLLNADRLA
                     LLRPSCYYINIGRGNVVDLDALGVALAAGELAGAVLDVFPTEPLPAEHPIWAAPNVVI
                     TPHISGVNRPEDVTNVFLDNLARYLAGEALVNVVDLKRGY"
BASE COUNT          438 a          831 c          888 g          431 t
ORIGIN      
        1 agaggacagg tcctctcgat gtctccgaat cccgaatccc ggttcccgaa tcccgctatt
       61 ccccgaagat cgacaacggt aaatacgctg tcaccgtcca gttcgggcgg tcgacgatct
      121 ggctgatctt gaggtcgacg acgacgctgg cgagcatgta ggcctcgggg cgggtcaggt
      181 cgtactcgcg ttgcatgtgg tcgagcatcg cgcgcagagc gattcgggct gcctcgcgga
      241 catccgggtc gaagccagtc gttgcgaagg cggcgccggg gatcgagagc ggctggccgt
      301 tggtgcgcag ccgtgggccg gggatcgtcg cgcccttctg caggtcaaag cgcagcgtga
      361 catcggagtt gatctcgata ccggtgccag tcacctcgcc gtcacccatc gcgaagtgga
      421 catcgccggt cgagaagagc gcgcccggtg ccagcaccgg caggtagagg gtcgcgcccg
      481 gctggaggtg gcggatatcc atgttgccgc cgttggtgcg cggcgggatc gtcgagagct
      541 ggcccggttc ggcctgggcg acgcccatca cccccggatg cttgccggcc gggacgcgga
      601 tgcccggctt gaagtcggcg tagtcgccgt tgcgcagatc ccagtaccag tgcatatcgt
      661 gatcgagatc gccgaggtcg tcgttgagca gcccaagccg ttcggcgcgc ggcgagcagg
      721 cgttgtagcc ccactgcgct ggcgcgatgt cgatgatctc gacgaccagc gtatcgcccg
      781 gctcggcgcc ttcgatcgcg accggcccgg tcagcgcatg gccgcccgtg atgctcgggt
      841 cacgctccgg atggctgcgg gccgggtagg cggcagcggc gatgatctcg atcagctcgg
      901 ggtcaagtcc cggctgggcc agctcgcgga cgttgcggca gacatgcccg tacgacgctt
      961 caagcgtctc gaagacgacc gtgtcacccg gctgaacggt gatgaccggc gggacggcat
     1021 tgctccagta ggtgtgaata tgctcgctgc tgacgcggtg cgtctggttt gcgctcatcg
     1081 tcgtgggttc cctctccgct catcggcccg gttggcgcgg gcattatcac cgattgcggc
     1141 acaagggagg agcaattgaa aaaagcagca gggaaggcag cgctaccttc cccaaccgcg
     1201 ccaccagacc gggtggaccc cacaatatcc acacccgggg tggaatggtt caggtatagc
     1261 atgcccagcg gcgaaacggc gtaacaaagg cgtcacagac tgggacgggt gacgcgtgac
     1321 gcgtgatgcg aaccagtcgg acgctgttca caaagtcctg caccgcagcc ctgtcatccc
     1381 gatgaaggag ggatctcgtc ctcggtcggt ccatgcccgc gtcaacgagc acgagatcct
     1441 tcgctgtcgc tcaggatgac aggggaagcg gcgcgggtcc tcatgaacgc cgttcgcctg
     1501 gctcgcctcc accatcccgc gtcacgcatc acgcgttacg cgtcacgcag atcaataccc
     1561 ccgcttcaga tcgacgacat tgaccagcgc ttcgccggcc agatagcggg cgaggttgtc
     1621 gaggaagacg ttggtgacgt cctcggggcg gttgacgccg gagatgtgtg gggtgatgac
     1681 gacgttcggc gcggcccaga tcgggtgctc ggcggggagc ggctcggttg ggaagacgtc
     1741 cagcacggcg ccggccagct caccggccgc cagcgctacc ccaagcgcgt cgagatcaac
     1801 gacgtttcca cggccgatgt tgatgtagta gcacgacggt ctgagcagcg ccagccgatc
     1861 cgcattgagc aggttggtcg attcggcggt atgcgggacg acggagatga cgaagtcgca
     1921 ctcaggcagg aagcggtcga tgtcggcgac ggcgtaggtc cgttcgacct cggcaaccgg
     1981 cgcgccgccg cggttgacgc cgatgacacg catcccgaac gcggcgcagc ggcgggcgat
     2041 ctcgcggccg atcgagccga ggccgatgat gccggccgtc tgcccacgta gtagcaccgg
     2101 ctcgaacggc tcccagcggc gctcctgctg ttgccgcagg atctgctgga tgcgctgggt
     2161 atgcgccagc atgtgggcca gcgcgtactc ggccatcgcc gtcgagaaga tgcccttcat
     2221 gttggtgacg atcacgccgt cgggaacgct cgcgccggcc agccgctcca ggccggcgcc
     2281 catcacctga aaccagcgca gccgctcggc ctgcggcagc agcgccatcg gaaactccca
     2341 ggcgaagagg atctccgcct gcggcagccg ctcgcggacc tgcgcctcgc cgtgagcggt
     2401 caggatcgtg agtcccggct gcgcctcagc gatccgagcc gcgacgatcg cgttctcgtg
     2461 ggcaacaccg ctgtggacca ggatagttgg agagtccggc atggctggac gcctttcgct
     2521 ctcttcgacc tgtcgtcctg aggccgcagc cgaaggacct ctccgcgtca acgacgcatg
     2581 gcgtcaaa
//
LOCUS       DASUGJ010000745         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_541228, whole genome shotgun sequence.
ACCESSION   DASUGJ010000745 DASUGJ010000000
VERSION     DASUGJ010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541228"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFV93_16850"
     CDS             complement(<1..203)
                     /locus_tag="VFV93_16850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5167079.1"
                     /translation="MVTTTLWLVRHGETSSNADGVFQGHLDVGLNQRGEEQAAAVGER
                     LACIDFDAVYASDLERAARTAEI"
     gene            complement(271..975)
                     /locus_tag="VFV93_16855"
     CDS             complement(271..975)
                     /locus_tag="VFV93_16855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5167080.1"
                     /translation="MRRGILVTVVLVVLLALLAACGEDDGVAGSSASADNPAATCSTA
                     TPLEGGSASYEGDPKEMQWNQPPAMALEEGRDYCATIKTSKGDIVVDLFEKDAPRTVN
                     NFVFLAQQGYYKGVPFHRIISGFMIQTGDPTGTGAGSPGYRFQDELPTNLNYTRGTLA
                     MANAGPDTNGSQFFICHADLTNSLQKNYSIFGTVTDGIDVVDAIASVPVGPSRSGEMS
                     SPKEPVSIESVEITVK"
     gene            complement(977..1567)
                     /locus_tag="VFV93_16860"
     CDS             complement(977..1567)
                     /locus_tag="VFV93_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX5167081.1"
                     /translation="MDITRENAWALLTEYVKGEPLLKHCLGVEASMREYARVFGEDEE
                     LWGFIGLLHDFDFEIHPTLDQHPQDGAPILRERGVPEHVIQSILSHADHLDVPRATRL
                     ELTLAAVDEMSGFVTAVALVRPTKSIDEVKPSSVKKKMKDKAFARAVNRDEMRHNAEA
                     LGVDFDEHVQHVIDAIATIAPRLGLNRTAETVEAGN"
     gene            complement(1623..2681)
                     /gene="queA"
                     /locus_tag="VFV93_16865"
     CDS             complement(1623..2681)
                     /gene="queA"
                     /locus_tag="VFV93_16865"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX5167082.1"
                     /translation="MTDSRPEPLPVDRLDYDLPEELIAQQPAEPRDSSRLLVVSRSDG
                     SLQDHIFRELPTLLQPGDLIVVNDTRVLPARLLARRSSGGRVELLLLARQADGSWQSM
                     ARPARRLRPGETLTLLDHSDALSGDHVEVIGREDDSVIVRFHDEADVQRHGRVPLPPY
                     IHESAPDPERYQTVYSREPGSAAAPTAGLHFTDAVMDACRERGVEFASVTLHVGLDTF
                     QPIKTADARDHQIHSEWFAVSAATLNAVAAAKREGRRVVAVGTTSVRTLESVADVVLA
                     NELPADAISGPTRLYITPGYQYRVVDVMLTNFHLPRTTLLLLVSAFAGEELMRDAYAH
                     AIRERYRFYSFGDAMLIV"
     gene            complement(2678..>2983)
                     /locus_tag="VFV93_16870"
     CDS             complement(2678..>2983)
                     /locus_tag="VFV93_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEX5167083.1"
                     /translation="ALSLLDVDELGLDDVDRRILLAIVEKFDGGPVGIDTLAAATSEE
                     TDTIMDVYEPYLIQLGFLQRTPRGRVATRRAYSHLGLPAPAMADETRQALLFGEQPE"
BASE COUNT          506 a          981 c          957 g          539 t
ORIGIN      
        1 atgatctcgg ctgtccgggc ggcccgttcc aggtcgctgg catagacagc gtcgaagtcg
       61 atacaggcca gccgttcgcc gacggccgca gcctgctctt cgccgcgctg gttcagccca
      121 acgtcgagat gtccctgaaa gaccccgtcc gcgttcgagc tcgtctcgcc atgccggacg
      181 agccagagtg ttgtcgtcac cacgacctgc catttccaga cgaaagggcg gccaaccggc
      241 cgccccgttg cgatcgacga aggtacggac ctacttgacc gtgatctcga cgctctcgat
      301 cgagaccggc tccttcgggc tggacatctc accgctgcgg ctggggccaa ccggcacgct
      361 cgcgatcgca tcgacgacat cgatgccgtc ggtcaccgtc ccgaagatcg agtagttctt
      421 ctggaggctg ttggtaaggt cagcatggca gatgaagaac tgcgagccgt tcgtgtccgg
      481 accggcgttg gccatcgcca gcgtgccgcg cgtgtagttg aggttggtgg gcagctcgtc
      541 ctggaagcgg tagccgggac taccggcgcc ggttccggtc gggtcaccgg tctggatcat
      601 gaaaccggag atgatccggt ggaacggaac gcccttgtag tagccctgct gggcgaggaa
      661 gacgaagttg ttgacggtgc gcggggcgtc cttctcgaac aggtcgacga cgatatcacc
      721 cttgctggtc ttgatcgtcg cgcagtagtc tctaccctct tcgagcgcca tcgccggtgg
      781 ctggttccat tgcatctctt tggggtcccc ttcgtatgaa gccgagccgc cttccagcgg
      841 cgtcgccgtc gagcaggtcg ccgcaggatt gtcggcggac gcggacgacc ctgcgactcc
      901 gtcatcctcg ccgcaggctg ccagcaacgc cagcaggacg accagaacaa ccgttacgag
      961 tattccacgc cgcatctcag ttccccgctt caaccgtctc ggccgtgcgg ttcaggccta
     1021 gacgcggcgc aatggtcgca atcgcgtcga tgacgtgctg gacgtgctcg tcgaagtcaa
     1081 ccccgagcgc ctcggcgttg tggcgcatct cgtcgcggtt gacggcgcgt gcgaaggcct
     1141 tgtccttcat cttcttcttg acggacgagg gcttgacctc atcgattgac ttggtcggtc
     1201 gcaccagcgc gacggcggtc acgaacccgc tcatctcatc gacggccgcc agcgtcagct
     1261 ccagccgtgt cgcgcgaggc acgtccaggt ggtcggcgtg actgaggatc gactgaatga
     1321 cgtgttccgg cactccccgc tcgcgcagga ttggcgcgcc gtcctgcgga tgctggtcca
     1381 gcgtcgggtg aatctcgaag tcgaagtcat gcaggaggcc gatgaaaccc cacagctctt
     1441 cgtcctcgcc gaagacacgg gcgtattcgc gcatgctcgc ctcgacaccg aggcagtgct
     1501 tgagcagcgg ctcccccttg acgtattcgg tgagcagcgc ccaggcgttc tctctggtga
     1561 tgtccatcgc ggtctgacgt ccctactttc ctctgcaaca gcgacgattt cgacgttcca
     1621 tcctacacga tcagcatcgc atcaccgaac gagtagaagc gatagcgctc gcgaatcgcg
     1681 tgcgcgtacg catctcgcat caactcttca ccggcaaagg ccgagaccag caacagcaac
     1741 gtcgttcgcg gcaggtgaaa gttcgtcagc atcacatcaa cgacccggta ctgatatcca
     1801 ggcgttatgt acagtcgggt ggggccggag atcgcatcgg ccggtagctc gttcgcaagc
     1861 accacgtccg cgaccgactc aagcgttcgc accgacgttg tgccgaccgc gacgacgcgc
     1921 cggccttcgc gcttcgcggc tgcaaccgcg ttcagtgtcg ccgccgacac agcgaaccac
     1981 tcggagtgga tctgatgatc gcgggcgtca gccgtcttga tcggctggaa cgtgtccagc
     2041 ccaacgtgca gcgtgaccga cgcgaactcg accccgcgct cgcgacacgc gtccatgacg
     2101 gcgtcggtga agtgcaatcc ggccgtcggg gctgccgccg agccaggttc gcggctgtag
     2161 acggtctggt agcgttccgg gtcgggtgct gactcgtgga tgtacggcgg caatgggact
     2221 cgcccgtgac gctgaacgtc ggcctcgtcg tggaaccgga cgatgaccga gtcgtcttcg
     2281 cgaccgatga cctcaacgtg atcgccgctc agcgcgtcac tatgatccag cagcgtcagc
     2341 gtctcaccgg gacgcagccg gcgggcgggc cgcgccatcg actgccagct cccgtcggcc
     2401 tgccgcgcga gcagcagcaa ctcgacccgc ccgccgctcg accgccgtgc caacagccgg
     2461 gcaggtagca cgcgggtgtc gttgacgacg atcaggtcgc cgggctggag cagtgtcgga
     2521 agctcgcgaa agatgtgatc ctgaagtgac ccatcggaac gcgacacgac cagcagtctg
     2581 gaggaatcgc gcggttcggc cggctgctgg gcgatcagtt cctctggcag atcgtagtcg
     2641 aggcgatcga ccggcaacgg ctcggggcgg gagtccgtca ctccggctgc tcgccgaaca
     2701 gcagcgcctg gcgcgtctcg tcggccatcg ccggcgccgg cagccccagg tggctgtagg
     2761 cgcggcgggt cgcgacgcgg ccacggggag tccgctgcag gaagccgagc tggatcaggt
     2821 acggctcgta gacatccatg atcgtatcgg tctcttcgct ggtggcggct gccagggtgt
     2881 cgatcccgac cgggccgccg tcgaacttct cgacgattgc cagcagaatc cgccgatcaa
     2941 cgtcgtccag ccccagttca tcgacatcga gcagcgagag cgc
//
LOCUS       DASUGJ010000746         4009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2068851, whole genome shotgun sequence.
ACCESSION   DASUGJ010000746 DASUGJ010000000
VERSION     DASUGJ010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068851"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFV93_16875"
     CDS             <1..560
                     /locus_tag="VFV93_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167084.1"
                     /translation="TWRVEVELGAISKRGGMDRLVEQQHSDAGKSILLLIPDVFFAVN
                     VRNSVRRVGYDPHVLKTADDLIVTAGEEQPALAVVDLAAVDGEAEWADIGDLAKAGLP
                     VLVFGSHKDVDGLRAAKAAGVTRVVSNGQFHREMDDLIQRYALASSCAIADEELDIDE
                     PLGSLPPGMAEHLPGSEPVRSAIEK"
     gene            612..1433
                     /locus_tag="VFV93_16880"
     CDS             612..1433
                     /locus_tag="VFV93_16880"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX5167085.1"
                     /translation="MRVFISVDIEGIAGVAGREEGSQGNPEYERARRLMTAEACAAVR
                     GIYNADPDAEVTVADAHGTFRNIIPELLDPRARLVRGKPRPLAMIEGVQNGVDAAVFV
                     GYHGRAGTGDSVLSHTFTGTLADVRVNGTSFGEIGLNAAVAGVYGVPLVLVTGDASVD
                     AEVEALLPGTRTAVVKRGIGALAADGLHPERACAIIEAAAAEVPERVASAKPFVVDGP
                     VALEVDVTIPAYADQALVVPGIKRLAGRTLGYDAPDYLTAYRITRLIGALSGLPL"
     gene            complement(1442..2722)
                     /locus_tag="VFV93_16885"
     CDS             complement(1442..2722)
                     /locus_tag="VFV93_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167086.1"
                     /translation="MAPDAGRPARRALLILALLLLTAGCTTPEIIRETPQPDPTETAT
                     LVPVETAIPPTGTPIVETPPRTIERIALATRVDDDGTPLDERSAIPQAAELFYLCVQV
                     RHVEQGTKFQAIWFEGDDILGRSEKLALEDASGPVWMAMQYRPIGELNPARPHAVELA
                     VDGQPIDRYIFRVGVGNPADAIAAAAFTSGFDALGKAISPQTVFPVDSPQLVFQVRIS
                     NQVDPAGMLFTTLWFRGDTQIGQRAPDPGGQDPRRLTFTFAPDSPFAAGNYRVALLLN
                     GNEVRSVPFVITTGPIPTPTVEPSQTAQPSVPAAQISDIVVASRIRRSTQEPLGGSLD
                     EFSVPAGEVAGLWVAIEVSDVIDDDTIEVDVLKDDEFYETVSLDTQDLESGWLAGRVE
                     LEAPSDSGEYYVYTFQASVNGDEPVETTLRLNAE"
     gene            complement(2712..3704)
                     /locus_tag="VFV93_16890"
     CDS             complement(2712..3704)
                     /locus_tag="VFV93_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5167087.1"
                     /translation="MTVQDAHRRFIDDDLRLSPRSRRTYDYSLRCFLEFVERDFGLDA
                     TTAPVTDLRIEHVTAFVSAMMPRDLRQPEDVSRMRTVQTQLAAIRKWYSYLAAYDFHP
                     DLPTDKLRTRVGAMLPRFSPPPPDIQLSDLERIVRHVRDLPHETKPQLELRRLKVRAM
                     VLFLFRTGVRVSELCALRRRDVDIENGTAGIYRAKGGKSRTVLFDNETAEALVAYWTA
                     RGDGGRGIGAHPAFSGRDKLGQPGTAISPRTVEHIVSQLCIGAGVEAEITPHSFRHGL
                     ASELVRRRVRESTVQTLLGHASPATTRIYVHKTAQEVSDEYQEAFGTYRKPDGA"
BASE COUNT          687 a         1230 c         1361 g          731 t
ORIGIN      
        1 aaacctggcg ggtcgaggtg gagttaggcg cgatctcgaa gcggggcggg atggacagat
       61 tggttgagca acagcatagc gacgcgggaa aatccatcct cttgctgatc ccggacgtgt
      121 tcttcgcggt caacgtccgt aactcagtcc ggcgtgtcgg ctacgatcca cacgttctca
      181 aaaccgcaga cgatctgatt gtcactgctg gtgaagagca gccggcgttg gcggtcgtcg
      241 acctggcggc cgttgacggg gaggctgagt gggccgacat cggcgatctc gcgaaggccg
      301 gcctgccggt gctcgtgttc gggtcgcaca aggacgttga cggattgcgc gccgccaagg
      361 cggccggtgt cacacgcgtc gtctccaacg gccagttcca ccgcgagatg gacgacctga
      421 ttcagcgcta cgcgttggcg tcgtcatgtg cgattgccga cgaagagctt gacatcgacg
      481 agccactggg tagcctccct cctggcatgg ctgaacacct tcccggcagt gagccggtac
      541 ggtccgccat cgaaaagtag ccagacagtt gccggagagt gtctgccgtt gtaacggacg
      601 gagaatgttg cgtgcgtgtc ttcatttcgg tcgatatcga agggatcgcc ggggttgccg
      661 gtcgcgagga aggcagccag ggcaatcctg agtacgagcg agcacgccgg ctgatgaccg
      721 ccgaagcctg cgcggccgtc cgcgggatct acaacgccga tcccgacgcc gaagtcaccg
      781 tcgcggacgc gcatggcacc ttccgcaaca tcattcccga gctactcgat ccccgcgcgc
      841 ggctcgtacg tggcaagccg cgtccgctgg cgatgatcga gggcgttcag aacggcgtcg
      901 acgctgccgt cttcgtcggc tatcacggcc gggccggaac cggcgattcc gtcctcagcc
      961 acaccttcac cggcacgctg gccgatgtcc gcgtcaatgg cacgtcgttt ggcgagatcg
     1021 ggctcaacgc ggctgttgcc ggcgtctacg gcgttccgct ggtgctcgtt accggtgacg
     1081 cgtcggttga cgctgaagtc gaggcgctgc tgcccggtac gcggaccgcc gtcgtcaagc
     1141 gtggcatcgg cgcgctggct gccgacgggc tgcacccgga gcgcgcctgc gcgatcatcg
     1201 aggcggcggc tgccgaggtg ccggagcggg tcgcgagcgc gaagccgttc gttgttgacg
     1261 ggccggtagc gcttgaggtc gatgtcacga tccctgccta cgcggaccag gcactggtcg
     1321 tccctggcat caagcggctc gccgggcgga cacttggcta tgatgcgccc gattacctga
     1381 ccgcctaccg catcacgcga ctgattggcg cgctctcagg tctaccgttg taggttgatg
     1441 gtcattcggc gttgagccgc agcgtcgtct caactggctc gtcgccgttg acgctggcct
     1501 ggaacgtgta gacgtagtat tcgccgctgt cggatggtgc ctccagttcg acgcgtcccg
     1561 cgagccagcc ggattcgaga tcctgcgtgt cgagcgacac ggtttcgtag aactcgtcgt
     1621 ccttgaggac gtcgacctcg atcgtgtcgt cgtcgatcac gtcactcacc tcaatcgcga
     1681 cccacagacc ggcgacctca ccggctggca ccgagaactc gtccagtgat ccacctagcg
     1741 gctcctgcgt cgatctgcga atacggctgg caacgacgat gtcactgatc tgtgccgccg
     1801 gcacgctcgg ttgcgcggtc tgagaaggtt cgaccgtcgg tgtcggtatc ggaccggtcg
     1861 tgatgacgaa cgggaccgag cgcacctcgt tgccgttcaa caacaacgcc acacggtagt
     1921 tgccggccgc aaatggacta tcgggtgcaa aggtgaaagt gagccggcgt gggtcctgac
     1981 caccgggatc tggtgcgcgc tggccgatct gggtgtcgcc ccgaaaccag agcgtcgtga
     2041 agagcatccc ggccggatcg acctggtttg agatgcggac ttgaaagacc agctgcggtg
     2101 aatcgaccgg aaagacagtc tgcgggctga tcgccttgcc gagggcatcg aagccggaag
     2161 taaacgcagc cgcagcgatc gcgtcggccg ggttgccgac gccgacccgg aagatgtagc
     2221 ggtcgatcgg ctggccatcg acggcgagtt cgaccgcgtg cggtcgggcc ggattcagtt
     2281 ccccgatcgg ccggtactgc atcgccatcc agaccggccc actggcgtct tcaagcgcga
     2341 gcttctcgct gcgcccgagg atgtcgtcac cttcgaacca gatcgcctgg aacttggtgc
     2401 cttgttcaac gtgccgaacc tggacgcaga ggtagaagag ttcggcggcc tggggaatcg
     2461 ctgagcgttc gtcgagcggc gtgccatcgt cgtcgacgcg ggtggcgagc gcgatccgtt
     2521 cgatcgtgcg tggcggggtt tcaacaatcg gcgtgccggt cggggggatc gcggtttcaa
     2581 ccggaactaa cgtggctgtc tcggtcggat caggttgcgg tgtctcgcgg atgatctccg
     2641 gcgttgtgca gccggcggtc agcaggagca gggcgaggat cagcagggca cgccgagcgg
     2701 gccggccggc gtcaggcgcc atccggcttt cgatacgtcc caaacgcctc ctgatactcg
     2761 tccgaaacct cctgggccgt cttgtggaca tagatgcgcg tcgtcgccgg agaagcgtgc
     2821 cccagcagcg tctgcaccgt cgactcccgc acccgtctcc ggaccagctc cgaagccagc
     2881 ccgtgccgga acgagtgtgg cgtgatctcg gcctcgacgc cggcgccgat gcagagctgg
     2941 ctgacgatgt gctcgaccgt tcgtggcgag atcgccgttc ccggctgacc aagtttgtca
     3001 cgtccggaga aggcaggatg ggcgccgatc ccgcgcccgc cgtcgccgcg tgccgtccag
     3061 taggcaacaa gcgcttcggc cgtttcgttg tcgaacaaga cggttcggct cttgccgccc
     3121 tttgcacggt aaataccggc cgtgccattt tcgatatcca cgtcgcggcg gcgtagagcg
     3181 cacagctccg atactcggac accggtccgg aacaggaaca ggaccatcgc gcgcaccttc
     3241 aggcggcgca actccagttg cggcttcgtc tcgtgcggta gatcgcggac gtggcgaacg
     3301 atccgctcca ggtcggagag ctggatatcc ggcggcggcg gcgagaagcg cggcagcatc
     3361 gcgccgactc gcgttcgcag cttgtcggtc ggaaggtcgg gatggaagtc gtaggcggcc
     3421 aggtaggagt accacttgcg gatcgccgcc agctgggtct ggacggtccg cattcgcgag
     3481 acgtcttcag gctggcgcag gtcgcgcggc atcatcgccg agacgaaggc cgtcacgtgc
     3541 tcgattcgga gatcggtcac cggcgccgtc gtcgcgtcga ggccgaaatc ccgctcaacg
     3601 aactccagaa agcagcgcag cgaatagtca taggtacgcc gagagcgcgg tgagagccgt
     3661 agatcatcgt cgatgaagcg acgatgggcg tcctggacgg tcatcgtact tgcagcatca
     3721 tcggcgatgt cgagtgttgg tggttgttca ctatgtacgt acatgatgct agtataggcg
     3781 tcgagtcagc ccggcgcaat cgggctgtac cgaaggacag gttcggcatc ggccggttgg
     3841 acgcccggaa ccagccagtc ggctgacgct gacgccgcaa ggggggcgta tgctggtgag
     3901 caaaacggag aacatccggc gcgcaatgag gcgcgacgga ggaggaggac actgatgttc
     3961 ctgatcattg gctacattgc gctcgtcgcc tatgccgccg ggctgtttg
//
LOCUS       DASUGJ010000747         7171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_957852, whole genome shotgun sequence.
ACCESSION   DASUGJ010000747 DASUGJ010000000
VERSION     DASUGJ010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957852"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /gene="eno"
                     /locus_tag="VFV93_16895"
     CDS             <1..261
                     /gene="eno"
                     /locus_tag="VFV93_16895"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5167088.1"
                     /translation="LTETFEAVNLAHRSGFTAVISHRSGETSDSFVADLVVATNAGQI
                     KTGAPSRMDRVEKYNQLLRIEEQLGEQAIFAGKEAFGPRRVS"
     gene            558..1805
                     /locus_tag="VFV93_16900"
     CDS             558..1805
                     /locus_tag="VFV93_16900"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX5167089.1"
                     /translation="MPWGQLSDGRGRRLALALPVLFNLGLLFGRPYLSTDLFTYLAHG
                     KLSLLDAGNPYVHQPTEVKQLPFGLDLLEYSGRSVAAVLAPYGPLWIHIVTAVVRLTE
                     DIAMAVLLLKTVVVAASLGTAALIWNLRRRVRPADQLLGTLIYLWNPVVIIEFAGEGH
                     NDALMIFFVVAALTFTVLARPMPGVVGIALGAMLKYVPLIFLPAQLKYVWRTQTNRLR
                     AAIQVALGGLAGLALAVLLFQPYWIGLDTLQGLRDQGETHTNPSTSGVTLWFLKRMLS
                     EDAAALVTRGLLSLSFAVFVSVMSWRVRNTESFLRASGFIALAYVLVAIPTYWPWYVS
                     LPIALMALTPHGVFRWMIILVSKFSRLTAPIEAMSVNDMLAWPTNVAMQSTAVLLPLI
                     VFLLLVARQWTDRRHLRTATTGA"
     gene            complement(1828..2040)
                     /locus_tag="VFV93_16905"
     CDS             complement(1828..2040)
                     /locus_tag="VFV93_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167090.1"
                     /translation="MNDDRERAEREDGDTPRASRDTAGKMALGMAIGVAMGSALGVAI
                     DNIAIGIAIGIAMGAALGVAFDVTKL"
     gene            complement(2059..2910)
                     /locus_tag="VFV93_16910"
     CDS             complement(2059..2910)
                     /locus_tag="VFV93_16910"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5167091.1"
                     /translation="MHQRLPGLELKSVGYRSGQFNDVLILNEDLVARFPMTADAERSL
                     AIEVAVLRRVHRVLPLPIPRPIVTEAEQTLQPTACMIYRMLPGTPLHQSTLNALRRTD
                     PDAFEYLGRQAGAFLRALHNIPLDDLDGIVPVADDAPYWQRMLAAFQDELFGYMRPDA
                     RQAVATTFESYLREPDALRWPPVLRHGDFGGANLLFDSSRRQLSGVIDFGSVAIGDPA
                     TDLAAISAFDERLALAMRPVYRELFSPAAHRRAAFYRSTFALQQALWALRAGDADEFQ
                     DGIAAYI"
     gene            complement(2961..4541)
                     /gene="gpmI"
                     /locus_tag="VFV93_16915"
     CDS             complement(2961..4541)
                     /gene="gpmI"
                     /locus_tag="VFV93_16915"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX5167092.1"
                     /translation="MSASHPGGPVVLVIMDGWGLEPAGPGNAIARANTPTFDRLWSAY
                     PHATLRTCGVDVGLPRGQMGNSEVGHLNLGAGFIVYQSITRIDLAIENGEFFRNPVLV
                     AAAQRASEGGRTLHLMGLLSDGGVHSHIHHLDALLDLAARTGVGRVAVHAFLDGRDTS
                     PTGGAGYLAETGMMIEAHGTGRIASVVGRYYAMDRDRRWQRTREAFDLLVNGEGIQTK
                     DAIAAVKQQYDEGVTDEFMKPIAVLHESGQPTTIRSGDVVIFFNFRADRARQLTQALV
                     GPPIEDHPFDAPDDLTFVMMTEYADYLPAQTAFPAIDVHFPLARVISEAGLRQFHTAE
                     TEKYAHVTYFLNGGREEPFEGEERELVPSPKVATYDLQPEMSAAGVGQATCEAIASGN
                     YAFVIVNFANCDMVGHTGVFAAAVAATEAVDTQLGKVINATLAAGGKAIVTADHGNAE
                     RMLVPGTDQPMTAHTTNPVPVILVAPDDASERRATLRPEGRLADVAPTVLQLLGIEPP
                     REMTGVSLLAHDTSGDAR"
     gene            complement(4565..5776)
                     /locus_tag="VFV93_16920"
     CDS             complement(4565..5776)
                     /locus_tag="VFV93_16920"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX5167093.1"
                     /translation="MSTRSFRDRYAATLGTAAGQPSEPADAPVWPLPRGARSSRNAEG
                     ECVVHDRVYGGPEIAETVEGLHACLRAISGRYVAARDMIFLDTETTGLSGGTGTHVFL
                     VGVGRFTGEALHVRQFFMRHPGDEKALLSALAAEIGEIGALVTYNGRAFDVPLLETRY
                     RMHGQSFVSPEQHIDLLSPARAIWKHRLPSCSLGTIERIVLGVERELDAPGWMIPQLY
                     FDYLRSQNVTMLEPVFEHNRTDIVTLARLTALVQMYEAGIMTPANDIDRLAVALHRLR
                     RHGEDEALEAIRQLWRLATVPADLRLRALREVSVALKRRRRHADAVDEWMAAQRDPSR
                     PVRLYAAEELAKYLEHGARDHAQALEVARRGADGAALARDTAALAAFERRLRRLERKM
                     QAAGQEDTHPA"
     gene            5976..7058
                     /locus_tag="VFV93_16925"
     CDS             5976..7058
                     /locus_tag="VFV93_16925"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX5167094.1"
                     /translation="MAYQVTLIPGDGIGPEVSDATRRVLEATGIGFEWDTQEAGVGAL
                     EKTGEVLPEVVLDSIRRNNIALKGPLTTPLGTGFRSVNVALRHELQLYANLRPARTFA
                     GVRSVFSNIDLVVVRENMEDLYAGVEFDTGADDTKEVLAVINRYSAKPVPASAAISIK
                     SITPENSERIVRFAFDYAIKNERKLVTSVHKANIMKFSDGLFLKVSQEVAKDFPQVDF
                     NDRIVDNMCMQLMQRPEDYDVLVMPNLYGDVLSDLTAGMVGGLGVAPSANIGEKAAIF
                     EPIHGSAPSHAGKNEANPSAMILSGAMMLRHLGELVAARAVEDAVAGVIAEGQQVTYD
                     LRPDRDRSKAAGTSDMASAIIARLGA"
BASE COUNT         1257 a         2358 c         2247 g         1309 t
ORIGIN      
        1 ctgacggaga cgttcgaggc cgtcaatctg gcgcatcgtt ccggcttcac ggccgtcatc
       61 tcgcaccggt cgggtgagac gtcagattcg ttcgtggccg acctggtcgt cgccaccaat
      121 gcgggacaaa tcaagaccgg agcaccatcg cgaatggacc gggtcgagaa gtataatcaa
      181 ctcctgcgaa ttgaagagca actgggcgaa caggcaatct tcgctgggaa ggaagcattc
      241 ggcccgcgac gggtgtcctg agaacgcaac cacctgggca cccatccgga gggagtccag
      301 gttgtggccg agccaccgtc aaaggagtca catgtcacgt ggcgtgacag cagtgggatc
      361 ctgcgggtcc tcctgatcgg gctctgtaac ggggcgctct acctcgcggt ttaccttctc
      421 caacgcggaa tctacctgaa cgggctggcc tctgaattgg gccagcccgc cgatcgtggc
      481 cggctggcgc tacaggccgc actctacatc ggctgcacga tcgcgctgtt cgcgctctac
      541 ggctgggtgt tgatgctgtg ccatgggggc aactcagcga tggtcggggg cggcggctgg
      601 cgctcgcgtt gcccgtcctg tttaacctgg gtttgttgtt tggccggcca tacctatcga
      661 ccgacctttt cacctatctc gcccacggca agctcagctt gctggatgcc ggcaacccct
      721 acgtgcatca accgaccgag gttaaacagc ttccgtttgg cctcgacctg ctcgaataca
      781 gtggacgctc cgtcgctgcc gtgctggcgc cgtacggccc gctctggatt catatcgtca
      841 cggccgtcgt ccggctgacc gaggacatcg cgatggcagt cctgctgctg aagaccgtcg
      901 tcgtcgcggc gagcctcggg acggctgcgc tgatctggaa cctgcgcagg cgagtacggc
      961 ccgccgatca gttgctcgga accctcatct atctgtggaa cccggtcgtc atcatcgagt
     1021 ttgccggcga agggcacaac gacgcgctga tgatcttctt cgtcgtcgcc gcgctgacct
     1081 tcacggttct cgctcgtccc atgcccggag tcgttggcat cgcgctcggt gcgatgctga
     1141 aatacgtgcc gttgatcttc ctgccggcgc agctgaaata cgtctggcgg acgcaaacga
     1201 atcgcctccg ggcggcgatt caggtcgcgc tcggtggcct ggccggactg gccctcgcgg
     1261 tactcctgtt tcaaccgtac tggatcggcc tcgacacgct ccagggcttg cgcgatcaag
     1321 gcgaaacgca taccaacccg tcgacgtcgg gtgtcacgct ctggtttctc aaacgtatgc
     1381 tctcggaaga cgctgcggca ctggtgactc gtggcctgct gtcgctgtcc tttgcggtct
     1441 tcgtcagcgt catgagttgg cgcgtccgca ataccgagag cttcctgcgc gcatccggct
     1501 tcatcgccct cgcctacgtg ctggtggcaa tcccaaccta ctggccctgg tacgtgtcct
     1561 tgccaatcgc gctgatggcg ctcacgccgc acggcgtctt ccgctggatg atcatcctcg
     1621 tctcgaagtt ctcgcggtta acggcgccga tcgaagccat gagcgtcaac gacatgctcg
     1681 cctggcccac caacgtcgcg atgcagagta ccgcggtcct gctaccgctg atcgtcttcc
     1741 tgttgttggt cgcccggcag tggaccgacc ggcggcatct caggaccgcc acgaccggtg
     1801 cgtgatacgc gctctgtagc ccgtcgatca cagcttggtg acatcgaatg ccacgccgag
     1861 tgcagcgccc atcgcgatac cgattgcgat gccgatggcg atgttgtcaa tcgcaacgcc
     1921 gagagcggag cccatcgcga caccgatcgc catcccaagc gccatcttgc cggcggtgtc
     1981 tctcgacgct cggggcgtgt cgccgtcttc tcgttcggct cgctctcgat cgtcattcat
     2041 tgtcagactc cattcgtctc agatgtaggc agcgatccca tcctgaaatt cgtctgcatc
     2101 gccagctcgc agtgcccaca gtgcttgctg cagcgcgaag gtgctgcgat agaacgctgc
     2161 ccgccgatgg gccgctggcg agaacagttc ccggtagaca ggccgcatag cgagtgccag
     2221 ccgctcatcg aacgccgaaa tcgccgccag atcggtcgcc ggatcgccga tggcgacact
     2281 gccgaagtcg atcactccag acagctgccg acggctcgaa tcgaagagca ggttcgcacc
     2341 gccaaagtcg ccgtggcgca gaaccggagg ccatcgtaat gcgtcaggtt cccgcagata
     2401 gctctcgaac gttgtcgcga ccgcctgcct cgcgtcgggt cgcatatagc cgaatagctc
     2461 gtcctgaaat gcagccagca tccgctgcca gtacggcgcg tcgtcggcga ctggcacgat
     2521 cccgtccaga tcgtcgagtg gaatgttgtg taaagcgcgc agaaatgcgc cagcctgacg
     2581 accaaggtat tcaaacgcgt cagggtctgt tcgccggagc gcattgaggg ttgactgatg
     2641 caacggtgta cccggcagca ttcgatagat catgcatgcc gtaggttgaa gcgtttgttc
     2701 ggcctctgtg acgataggcc gggggatcgg aagcggcaat acgcggtgca cgcgccgcag
     2761 aacggcaacc tcgatcgcaa gcgagcgctc tgcgtccgcc gtcattggaa aacgggcaac
     2821 cagatcctcg ttcaggatca gcacgtcgtt gaactgaccc gatctgtatc cgaccgattt
     2881 gagctcaagt cccggaaggc gctggtgcac gacctgcaac aggtcgcgct gccgatcgtc
     2941 ctcccgctca gtgacgagct tcaacgcgcg tcgccgctgg tatcgtgcgc gagcagcgag
     3001 acgccggtca tctccctcgg cggctcgatc ccgagcaatt gcaggacggt tggcgcgacg
     3061 tccgccagcc gcccctcggg gcgcagcgtc gctcttcgct ccgacgcgtc atcgggcgcg
     3121 acgaggatga cgggaaccgg gttggtcgtg tgggccgtca tcggctggtc agtgccaggc
     3181 acaagcatcc gttcggcgtt gccgtggtcg gccgtcacga tcgccttgcc gccagccgcc
     3241 agcgttgcgt tgatgacctt gccgagttgg gtatcgaccg cctcggtcgc ggcgacggct
     3301 gctgcgaaga cgcctgtatg gccgaccatg tcgcagttgg cgaaattgac gatgacgaac
     3361 gcgtagttgc cgctcgcgat cgcctcacag gtcgcctgac caacgccggc tgcgctcatc
     3421 tccggctgca agtcataggt cgccaccttc ggggaaggaa ccagttcgcg ctcctcgccc
     3481 tcgaacggct cctcacgccc gccgttgagg aaataggtga cgtgcgcgta cttctcggtc
     3541 tcggctgtgt gaaactggcg caaccctgcc tcggagatga cgcgagccag cgggaagtgc
     3601 acgtcgatgg cggggaaggc ggtctgagcc ggcaggtagt ccgcgtactc cgtcatcatc
     3661 acgaacgtca ggtcgtccgg cgcgtcgaac ggatgatcct cgatcggtgg cccgaccagc
     3721 gcctgcgtca gctgtcgcgc ccgatctgcc cgaaagttga agaagatgac gacatccccg
     3781 ctccggatgg tcgttggctg gccggactcg tgaagcaccg cgatcggctt catgaactcg
     3841 tcggtgacgc cctcgtcgta ctgctgcttg acggcggcga tcgcgtcctt cgtctgaatg
     3901 ccctcgccgt tgacaagcag gtcgaacgct tcccgcgtcc gctgccagcg tcgatcgcgg
     3961 tccatcgcgt agtaacggcc gacgaccgaa gcgatccggc ctgtgccatg cgcctcgatc
     4021 atcatgccgg tttccgccag atagcccgcg ccgccagtcg gcgacgtgtc gcgcccgtcc
     4081 agaaatgcat gcaccgccac ccgtccgacg ccggtccggg cggccaggtc aagcagcgcg
     4141 tcgaggtgat gaatgtgcga gtggacgcca ccatctgaga ggagtcccat cagatgcagc
     4201 gtccgaccgc cttcgctggc acgctgggca gcggcgacca gaaccggatt gcggaagaat
     4261 tcgccgttct cgatcgccag gtcgatgcgc gtgatcgact gatagacaat gaatcccgct
     4321 ccaaggttaa gatggccaac ctccgagttg cccatctggc cgcgcggaag cccgacgtct
     4381 accccacagg tgcgcagcgt cgcatgtgga taggccgacc acagccggtc aaacgtcggc
     4441 gtgttggcac gagcgatggc gtttccggga ccggcaggtt caagccccca gccgtccatg
     4501 atcaccagca cgaccggacc acctggatgc gatgcgctca ctgtctcctc atctcgttcg
     4561 tgtgttatgc ggggtgcgtg tcttcctgac ctgccgcctg catctttcgt tccagccgcc
     4621 ggagccgccg ttcgaaggcg gccagcgcgg ccgtgtcccg cgccagtgcc gcgccgtcgg
     4681 caccacgccg tgcgacttcc agtgcctgcg cgtgatcgcg tgcgccatgt tcgaggtact
     4741 tcgccagctc ctcggccgcg tacagtcgca ccggccggct cgggtcgcgc tgggctgcca
     4801 tccactcgtc aacggcgtcc gcgtgtcggc gccgacgctt gagcgcgacc gacacctcac
     4861 gcagggcgcg caggcgcaga tcggccggaa cggtcgccag ccgccagagc tgccggatcg
     4921 cctccagcgc ctcgtcctca ccgtggcgtc ggagccggtg aagggcgacc gccagccggt
     4981 cgatgtcgtt ggccggcgtc atgatcccgg cctcgtacat ctgtaccaac gccgtcagcc
     5041 gcgccagcgt cacgatgtcg gtccggttgt gctcgaagac cggttccagc atcgtaacgt
     5101 tctgcgaacg caggtagtcg aagtagagct gcgggatcat ccagccgggg gcgtccagct
     5161 cccgctcgac acccagcacg atccgctcga tcgtgccgag tgagcaggac ggcagccggt
     5221 gcttccagat cgcgcgggcc ggcgaaagca ggtcgatatg ctgctccggc gagacgaacg
     5281 actgcccgtg catgcggtag cgggtctcca gcagcggcac gtcaaacgcg cgcccgttgt
     5341 aggtgaccag cgcgccgatc tccccgatct cggccgcgag cgccgacaac agtgccttct
     5401 catcgcccgg atgacgcatg aagaactgcc ggacatgcaa cgcttcaccg gtgaagcgtc
     5461 caacgccaac caggaagaca tgcgtaccgg ttccacccga cagcccggtc gtctcggtgt
     5521 cgagaaagat catatccctc gcggcgacgt agcgtcccga gatagcgcgc aggcaggcgt
     5581 gcagaccctc taccgtttcc gcgatctcgg ggccgccata gacgcggtca tggacgacgc
     5641 attcaccctc agcgttgcgc gacgatcgtg ctcctcgcgg aagcggccag accggcgcat
     5701 cggcaggctc gctcggctgc ccggcagccg ttccgagcgt cgccgcgtac cgatcgcgaa
     5761 agctacgtgt gctcatggcg cgccatccga gttcatacca gcattgtaat ccgcgcttgc
     5821 gcgacggaca gcctgcggga gcccggctgg tttgacactc cttttcaacc acgcatagaa
     5881 tcgcgtcgtg aggttgcagg aacgtacatt gcggcaccag cctcgcacgg attggtcgcg
     5941 gccagcggca gggtacagaa tcaggagcgc ggagcatggc gtatcaggtc acgttgatcc
     6001 ccggagacgg tatcggccca gaggtatcgg acgcgacacg acgcgtgctt gaggcaacag
     6061 gaatcggctt cgagtgggac acgcaggagg caggtgtcgg tgcgctggag aagactggcg
     6121 aggtcctgcc agaggtagtc ctcgattcga tccgcaggaa caacatcgcc ctcaagggac
     6181 cgctgacaac gccgctcgga accgggttcc gcagcgtcaa tgtcgccctg cgtcatgagc
     6241 tccagctgta tgccaatctg cgtccggcgc gaaccttcgc cggcgtgcgc tcggtcttct
     6301 ccaacatcga cctcgtcgtc gtccgcgaga atatggaaga cctctacgca ggcgtcgagt
     6361 tcgacactgg cgccgacgat acgaaggaag tgctcgccgt catcaatcgc tactcggcga
     6421 agccggtgcc ggccagcgcc gcgatctcga tcaagtcgat cacgcccgag aactcggagc
     6481 ggatcgtccg cttcgccttc gactacgcca tcaagaacga gcgcaagctg gtcacgtcgg
     6541 tccacaaggc caacatcatg aagttcagcg acgggctgtt cctcaaagtc tcgcaggagg
     6601 tcgccaagga tttcccgcag gtcgatttca acgaccggat cgtcgacaac atgtgcatgc
     6661 agctcatgca gcggcctgag gactacgacg tgctcgtgat gccgaatctg tacggcgatg
     6721 ttctctccga cctgacggcc gggatggtcg gcgggctcgg tgtggcgccg agcgccaaca
     6781 tcggcgagaa ggcggccatc ttcgagccga tccacggcag cgcgccgtcg cacgcgggca
     6841 agaacgaggc caacccctcg gcgatgatcc tctccggcgc gatgatgctg cggcacctgg
     6901 gcgaactggt cgccgcgcgt gctgtagaag acgcggttgc cggggtgatc gcggagggcc
     6961 aacaggtgac ctacgatctg cgtcccgacc gggaccgcag caaggcggcc ggcaccagcg
     7021 acatggcctc ggcgatcatc gcccgtctgg gcgcctgatc acccctcgcc ccggtacgaa
     7081 gctcgactgg gctgcgctcc cagccgcacg gttgctgtcg gggaatggga tcagatcgaa
     7141 cgacgcgatc gaaaacgtgt tgatctaaca t
//
LOCUS       DASUGJ010000748         7699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_957874, whole genome shotgun sequence.
ACCESSION   DASUGJ010000748 DASUGJ010000000
VERSION     DASUGJ010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957874"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1514)
                     /locus_tag="VFV93_16930"
     CDS             complement(153..1514)
                     /locus_tag="VFV93_16930"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX5167095.1"
                     /translation="MTTTPPPASLTDVPPAALLGEIETILAEVVTGLTPVVAPDRAPH
                     SPGRPPILPAFLVWGTLLVGVVRGLRSQADCFRLLGIGLWGHAPIEVSEQALRHRWAR
                     AGTGPLEQCFAAVSALVDARLAPWRARWGGPTLAPFASDVVVVDETTLDQVARSIPAL
                     RGVPPGDDRLLPGKLAGVFDVRRQSWRVLRWVAAIHQNEKVIARELIAALAPGSLILT
                     DLGYFGFQWFDDLTDAGHWWLSRLRAKTSTVVLHTFLTTDTYRDQLVWLGAYRADRAK
                     HAVRLVEVKVGGHWHRYLTNQLDPVVFPAADVVALYARRWDIELAIRLVKDHLGLGLL
                     WSANPLLIQQQVWAVLCIAQILQGLRLEIAARAEVEPFEVSIELLVRWAPRLAAEGRD
                     WLTIFVEDGRRLGFIRPSRRLQLVLPAVAPDALTPLPPDCIRTRIPRYAHRNAGPRST
                     AVA"
     gene            complement(1721..3073)
                     /locus_tag="VFV93_16935"
     CDS             complement(1721..3073)
                     /locus_tag="VFV93_16935"
                     /inference="COORDINATES: protein motif:HMM:NF025273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHL domain-containing thioredoxin family
                     protein"
                     /protein_id="HEX5167096.1"
                     /translation="MNCVHILPQLRELEARYADRLVVIGVHSAKYPAEEESRHLAAAV
                     QRLEVEHPVINDVGRRIWDAYAVRAWPSVMLVDPAGNVVAKHEGEFTVDMFEPFLEGG
                     FAEWERAGLLTPGPLAGLTHPASPTTPLAFPGKVVVAGERLYIADTNHNRIVVTTLDG
                     KVTQVMGSGLAGLADGVRQAAAFNHPQGLAVDGDTLYVADTDNHAIRRVDLSSGVVTT
                     IAGTGEQAHSFMSGGPALRTSLNSPWDIVLLDSVLWITMAGFHQLWWHRPGSGEIRRF
                     AGTGHEGLRDGSTSSAWLAQPGGLAAYGPQALVFTDSETSSVRTADLPGHGDGRVVTL
                     VGEDLFEFGDIDGGPEVARLQHPLGVAYDEPNGVLYVADTYNNRIKRLDPRTRRIESW
                     LGDGQAGDADGIGPEARFFEPAGLSVGAGALWIADTNNHAIRRADLATGEVTTVPIDG
                     "
     gene            complement(3074..3211)
                     /locus_tag="VFV93_16940"
     CDS             complement(3074..3211)
                     /locus_tag="VFV93_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167097.1"
                     /translation="MSERYLGSVRAPEFPVGAEWFNVEQPLTLAELRGRLVLLDFWTY
                     C"
     gene            3516..4643
                     /locus_tag="VFV93_16945"
     CDS             3516..4643
                     /locus_tag="VFV93_16945"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5167098.1"
                     /translation="MKYRFGLLPAIILLLGVGLLLPATAGAEEEEVGPSQVYFPTTGH
                     TVADEFLDYWRHNGGMEIYGLPLSEVVTDPVTKLPTQYFERAVFEWHENNPPEWKVLL
                     RRLGAEMTTGRAKEQPFQRIPDYQTPGCNYFPETGHRVCAGFKAYWDSYGAIPAFGYP
                     LSEEFTERNADTGQTYIVQYFERARFEYHPENKGTIYEVLLGRLGDVEAKQDKVNTAP
                     VPRPDGVEDYDPDLWYIPAPPPPPPPAGAPVWEAKWIEIDISDQYMQAWEYDVVVNGT
                     YVSTGIPGYETPIGTFTIFSKLRYDDMTSGLAVPPGEYYYVEDVPHVMYFADGGYAIH
                     GAYWHNMFGTPFSHGCVSTPLGQAEWFYNWAPYGTTVWIHY"
     gene            5124..6338
                     /locus_tag="VFV93_16950"
     CDS             5124..6338
                     /locus_tag="VFV93_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167099.1"
                     /translation="MAEIVHNVDLAPRHKGFEPCAVRGYDVALFTGNWFAAYSTDSGN
                     TFRSLDPNGLLRNEGESLCCDQRVEFVPSINTFAWVLLSNEGPFALAVASPDEIRNSG
                     GRNWTYYHVTPELFGFPGSTFDYPQISFGYDYLYLTVDVVGGDGDGRAVIMRMPLGQV
                     AERQTLDVQYITPQQWYICPSHNTRDVGVFANFVSDSEIRVYTWTEDPSAWVFHFDVP
                     VATVPTLDFSSLTPDNDDWLPPTSKIDWNVTGAARSSRYLYLAWSAGKLYADGTPSPI
                     TQPHVEWAQIDIAARSLVTQRYIWNREFAFAWPSLAANWERDTAIAISLCWGGGEHYP
                     QHAVGTIYPGGNRLLSTTSGRTAGAGGHYNDIRMDYPDSQQFVAAGFITAKDNASPPK
                     LVNHPHFVIVEP"
     gene            complement(6442..>7699)
                     /locus_tag="VFV93_16955"
     CDS             complement(6442..>7699)
                     /locus_tag="VFV93_16955"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX5167100.1"
                     /translation="MAGWWTRVRTTGNRQAGALLLLLALIAAACSGPDDARSPTPTAT
                     SSAVVSPTVAAPAPETSTQVATQPGSATPTATTAPVVATPSPTAAPEGAVCDTGVELL
                     AVSDALDKVTYENQEVGGLSALAWSGEGSTYDTLSDRGGRVYRLDFPAPGDPSLAATI
                     QLLDEDGNPDTDIDGEGLAILPNGDLLVSSEAGPSIREYTPDGALVAELPVPERFLNA
                     PAGRATQNLTFESLALSPSGQRLFTAVEDRLEGDGRTDEGNSRIRVLRYDLIDGAFQP
                     AAEYVYLTEPDQAVSEMVAISDTELLVLERSLSLFMGFTARVFHADLDGADDVTAVES
                     LEDADAQPATKELLVDIADCPSGDAAVIDGFNPLLENFENMTLGPPQPDGRSTLIVGS
                     DDNYQSLQTTRFLIFAIDADRLPPSS"
BASE COUNT         1363 a         2659 c         2430 g         1247 t
ORIGIN      
        1 gcaacactcg atatccagac ctttgaggcc ggcgattgcc cgctctgccg gcaaggaacg
       61 ccactgacga agccaggctc gaccgccgtt tccacgtctc ccgcgtaggg gcgatggccg
      121 cagcccatgc gtgccaccat aacataacgt tgtcatgcga cggccgtgct gcgtggccca
      181 gcgttgcggt gggcgtagcg cgggatccgc gtccggatgc agtcaggcgg cagcggggtc
      241 agggcgtccg gtgccaccgc gggcagcacc agctgcagcc gtcgcgacgg ccggatgaag
      301 ccgagccgac gaccatcctc gacgaagatc gtcagccagt cgcgaccctc cgcggccagc
      361 cgtggcgccc agcgtaccag cagctcgatc gagacctcga acggctcgac ctcggcgcgg
      421 gcggcaatct ccagccgcag gccctgcagg atctgggcaa tgcacaagac cgcccagacc
      481 tgctgctgga tcagcagcgg gttggcactc cacagcagcc ccagcccgag atggtccttg
      541 accagtcgga tggccagttc tatgtcccag cggcgcgcgt agagggcgac gacatcggcg
      601 gccggaaaga cgaccggatc gagctggttg gtgaggtagc ggtgccagtg accgcccacc
      661 ttgacctcaa cgagtcggac ggcatgcttg gcccgatcag cccggtacgc ccccagccag
      721 acgagctggt cccgataggt gtcggtcgtg aggaacgtgt gcagcacgac cgtgctggtt
      781 ttggcgcgca gccggctgag ccaccagtgc ccggcatcgg tcaggtcatc gaaccactgg
      841 aagccgaaat agcccagatc ggtcaggatc aggctgcccg gcgccagcgc agcgatcagt
      901 tcgcgggcga tgaccttctc attctggtgg atggccgcca cccagcgcag cacccgccag
      961 gactggcgac gcacgtcaaa cacgccggcc agcttgcccg gcagcagccg gtcatcgccg
     1021 ggcgggacac cgcgcaaggc cgggatggag cgcgccacct gatcgagggt ggtctcatcc
     1081 acaacgacga catcgctggc aaacggcgcc agcgtcgggc cgccccagcg ggcacgccac
     1141 ggcgccagtc gtgcgtcgac cagcgcactg acggccgcga agcactgctc caacggtccc
     1201 gtcccggccc gtgcccagcg atggcgcagc gcctgctcgc tgacctcgat tggcgcgtgg
     1261 ccccacagcc cgatccccag cagccggaag cagtcggcct ggctgcgcag cccgcgcacg
     1321 accccgacca gcagcgtccc ccagaccagg aaggccggca ggatcggcgg acgaccgggc
     1381 gagtgcggtg cgcggtccgg cgcgacgacc ggcgtcaggc cggtcacgac ctccgccagg
     1441 atcgtctcga tctcgccgag caacgcggcc ggggggacat ccgtcaacga cgctgggggc
     1501 ggcgtggtcg tcatcgtggt ctccgttcct gccagacaca tccgtggcac acagcctacc
     1561 gcaccccggc cggccgccgc cgcgtgtcgt tatgttatgg tggcacgcat gcagccgcag
     1621 cctcgccctg cctggatcgc gcccaaccag ggcgaggctg cggccgtcgc ccctacgcga
     1681 tacacccctc ctgtcgtcct gagccacagt gcgtgactcg tcacccatcg atcgggacgg
     1741 tcgtcacctc accggtcgcc aggtccgcgc ggcggatcgc gtggttgttc gtgtcggcga
     1801 tccagagtgc cccggccccg acgctgaggc cggcaggctc gaagaagcgc gcttccggcc
     1861 cgatgccgtc ggcatcaccg gcctgcccgt cgccaagcca gctctcgatc cttcgcgtgc
     1921 gcgggtcaag tcgcttgatc cggttgttgt aggtgtccgc gacatacagc accccgttcg
     1981 gctcgtcata cgcgacgccg agcgggtgtt gcagccgggc gacctccggg ccgccgtcga
     2041 tatcaccgaa ctcgaacagg tcctcgccaa ccagcgtcac gacccgaccg tcgccgtgac
     2101 ccggcaagtc ggccgtccgg accgaactgg tctccgaatc ggtgaacacc agcgcctgcg
     2161 ggccgtaggc ggccaacccg cccggctgcg ccagccacgc gctggatgtc gagccgtcgc
     2221 gcagcccctc atgtcccgtc ccggcgaagc ggcgaatctc accgctacca ggccggtgcc
     2281 accagagctg gtggaagccc gccatcgtga tccagaggac actgtcgagc aggacgatgt
     2341 cccacggcga gttgaggctc gtccgcaacg ccgggccgcc ggacatgaag ctgtgggcct
     2401 gctcaccggt cccggcgatc gtcgtcacga cgccgctcga aagatccacc cgccggatgg
     2461 cgtggttgtc ggtgtcggcg acgtagagcg tgtcgccatc aaccgccagt ccctgtgggt
     2521 ggttgaaggc ggccgcctgc cgcacgccat ccgccagccc tgccagaccg gagcccatca
     2581 cctgcgtcac cttgccatcg agcgtcgtca cgacgatccg gttgtggttc gtgtcggcga
     2641 tgtagagccg ctcgccggca acgacgacct tgccaggaaa cgccagcggt gtcgttggcg
     2701 acgccgggtg cgtcagcccc gccagcggcc caggcgtcag cagcccagcc cgctcccact
     2761 cagcgaagcc accctccagg aacggttcga acatatcgac cgtgaactcg ccctcgtgct
     2821 tggcgacgac gttcccagcc ggatcgacca gcatcaccga tggccaggcc cggacggcgt
     2881 aggcgtccca gatccgtcga ccgacatcgt tgatgaccgg gtgttcgacc tccagccgct
     2941 ggacggccgc cgcgaggtgg cggctctcct cctcggccgg gtacttggcg ctgtggacgc
     3001 cgatgacgac cagccggtcc gcgtagcgcg cttcaagctc ccgcaactgc gggagaatat
     3061 ggacacagtt gattcagcaa taggtccaga agtcgagcag caccagccgc ccgcgcaact
     3121 cagccagtgt caacggctgc tcgacgttga accactccgc cccgaccggg aactccggcg
     3181 cccgcacgct ccccagatac cgctccgaca tgctgtcctc cctgcctgcc gatacgccgg
     3241 gcataacgat actgctgatt gcgggattcg ggactcggga ctcggggctg agcgacatgg
     3301 catgttcttc atggacatcc aatgttggat acccccgaat cccgaatccc gaatcccgcc
     3361 catagacacc cgccccccga caccctacaa tcgggacgga atgccatgta ggcattgatt
     3421 gtctgtttgg cacgcgattt gcgtgtcgcg atgccgcgtt cgtgattaca atgacaggtc
     3481 taccgggcga gatcacaatc agatgggggt tggcaatgaa gtatagattc gggttactgc
     3541 ccgcaatcat cctgttgctc ggtgtcggtc tgctgttgcc ggcgaccgcc ggcgccgagg
     3601 aggaggaagt cgggccgtcg caggtctatt tcccgacgac cggccacacc gtcgcggacg
     3661 agttcctcga ctactggcga cacaacggcg ggatggagat ctacggcctg ccgctaagcg
     3721 aggtcgtcac cgacccggtc acgaagctgc cgacgcaata ctttgagcgc gccgtcttcg
     3781 aatggcacga gaacaacccg ccggagtgga aggtgctcct gcgccggctc ggtgccgaga
     3841 tgacgaccgg tcgcgcgaag gagcagccct tccagcgcat ccccgactac cagacgcccg
     3901 gctgcaacta cttccccgag accggccacc gcgtctgcgc cggtttcaag gcctactggg
     3961 attcgtacgg cgcgataccc gccttcggct atccgctctc ggaggagttc accgagcgca
     4021 acgccgacac cggccagacc tacatcgtcc agtacttcga gcgcgcccgc ttcgaatatc
     4081 accctgagaa caagggcacg atctacgaag tgctgctcgg ccggctcggc gatgtcgagg
     4141 cgaagcagga caaggtcaat accgcccccg ttccgcgccc cgatggcgtc gaagactacg
     4201 acccggatct ctggtacatc cccgctccgc ccccaccacc accgccggcc ggcgcaccgg
     4261 tctgggaggc gaagtggatc gagatcgaca tctccgacca gtacatgcag gcctgggagt
     4321 acgacgtcgt cgtcaacggc acctacgtct cgaccggcat ccccggctac gagacgccga
     4381 tcggcacctt caccatcttc tccaagctac gctacgacga catgaccagc ggcctggccg
     4441 tcccgcccgg cgagtactac tacgtcgagg acgtcccgca cgtcatgtac ttcgcggacg
     4501 gcggctacgc gatccacggc gcctactggc acaacatgtt cggcacaccg ttcagccacg
     4561 gctgcgtcag caccccgctc ggccaggccg aatggttcta caactgggcg ccgtacggca
     4621 cgaccgtctg gattcactac tgatcgcgtc cggacgcaat ttgcgggacg cgtgtagcac
     4681 gcgtcccgcg ctgtaaatcc tctgtttgtg cagtctccgg atgggttcgg gttgcgttcc
     4741 gctagccccg cggtggactt gcggtcatcc aatgccgcga gttggtgcat ctcgacggtg
     4801 gccggctaac gacggctgct agaatgcccc tatcgttcag tgccacagag gtcgtcctgc
     4861 cgactattga tctcgacgcc gtcacccgcc tgcatgatga gggcgagagg cgttagaagg
     4921 cgagagcatc atgaccgtcg aagaggaacg aatcgaacca caaccggcgc aagtcgaacc
     4981 cacccaagtc gatatccctg acaagcccat tgacggaggt cggggaggcg aagacgagcg
     5041 aagccgtcgt cataccccgc cgcgcggccc atttcgaccg atgaaggatc cgggcgagcc
     5101 gacgaccgtc gaggaggttc caggtggcag aaatcgtcca caacgttgac ctcgcgccac
     5161 gacacaaagg cttcgaaccc tgcgcggttc ggggctacga tgtcgcattg ttcaccggca
     5221 actggttcgc cgcctattcg acagacagcg gcaatacgtt tcgatccctc gaccctaacg
     5281 gcctcctgcg caatgagggc gagtcactct gctgcgatca gcgggtagag ttcgtgccca
     5341 gcatcaacac attcgcgtgg gttctcctga gcaacgaagg tccgtttgcc ctcgcagtcg
     5401 ccagccccga cgagatccgg aactcaggag ggcgcaattg gacgtattat cacgtcactc
     5461 cggagctatt tgggttcccc ggtagcacgt tcgactatcc acagatctcg ttcggctacg
     5521 actacctgta cctcaccgtc gacgtggtgg gaggcgacgg cgacggccgg gccgtgatca
     5581 tgcggatgcc gctggggcag gtcgctgaac gtcagacact cgacgtccag tacatcacgc
     5641 cccagcagtg gtacatctgt cccagtcaca atacccgcga cgtcggcgta ttcgcgaact
     5701 ttgtgtcgga cagtgagatc cgcgtctaca cctggaccga agatccgagc gcctgggtct
     5761 tccatttcga tgttcctgtc gccacggtgc caacgctgga cttctcgtcg ctgacgccgg
     5821 acaacgacga ttggctcccg ccgacgtcca agatcgactg gaacgtcacc ggtgccgcgc
     5881 gcagtagtcg ctatctgtat ctggcatgga gcgccggcaa gctctacgcg gatggcacgc
     5941 cgtctccgat cacccaaccg cacgtcgagt gggcacagat cgacatcgcg gcacggtcgc
     6001 tggttacgca gcgctacatc tggaaccgtg agttcgcgtt cgcctggcca tcgctggcgg
     6061 cgaactggga gcgtgacacg gcaattgcca tttcgttgtg ctggggcggg ggcgagcact
     6121 atccgcagca cgcggttggg acgatctatc ccggcgggaa tcgtctgctc agcacgacga
     6181 gcgggcgaac ggctggggcc ggaggtcact acaacgacat ccgcatggac tacccagatt
     6241 cgcaacaatt cgtggcagcc gggttcatca cagccaaaga caacgcgtcg ccacccaagc
     6301 tggtgaacca tccccacttc gtcatcgtgg agccataggc tcgatccatc acgcggtcat
     6361 cgttgtggac gctgtgaagg cgagggacct ctccagttcg acgccgaacg aggctgacgc
     6421 ggagaagtcc ttcgctgtgg ctcaggacga cgggggcaac ctgtcagcat caatcgcgaa
     6481 gatcaggaat cgggtggtct ggagcgactg gtagttgtcg tcgctgccga cgatcagggt
     6541 ggagcggccg tcgggctgtg gcgggccgag cgtcatgttc tcgaagttct ccagcagcgg
     6601 gttgaagccg tcgatcacgg ccgcgtcgcc gctcgggcag tcggcgatat cgaccagcag
     6661 ctccttcgtc gccggctggg cgtcggcatc ctccagggat tcgaccgccg tcacgtcgtc
     6721 ggccccgtct agatcggcat ggaagacgcg ggccgtgaac cccatgaaca gcgacagcga
     6781 gcgttccagc accagcagct cagtatccga gatcgcgacc atctccgaga cagcctggtc
     6841 cggctcggtc aggtagacgt actccgccgc cggctggaac gcgccgtcga tcaggtcgta
     6901 gcgcaggaca cgaatccggc tgttgccctc atctgttctg ccatcccctt cgagccggtc
     6961 ctcgacggcg gtgaagagcc gctgccctga cggcgagagc gccagcgact cgaaggtcag
     7021 gttctgcgtc gccctccccg ccggcgcgtt gaggaagcgc tccggcaccg gcagctccgc
     7081 gacgagcgcg ccgtccggcg tgtactcacg aatcgacggg ccagcctcgg atgacaccag
     7141 cagatcgcca ttcggcagga tcgccagccc ctcgccgtcg atgtcggtgt ccgggttgcc
     7201 gtcctcgtcg agcagttgga tcgtcgccgc caggctcgga tcgcccggcg cgggaaagtc
     7261 cagccggtag actcgcccac cgcgatccga gagcgtgtcg taggtactgc cctcgccgct
     7321 ccaggccagc gcagacaggc cacccacctc ctgattctcg taggtcacct tgtcgagcgc
     7381 atccgagacc gccagcagct caacacccgt gtcgcaaacg gcgccttccg gcgcggcagt
     7441 tggtgatggc gtcgcaacga cgggtgcggt cgtcgcagtc ggtgtggcgg agcctggttg
     7501 cgtcgccacc tgggtagaag tttcgggggc gggcgccgcg acggtcggcg acacgacagc
     7561 gctggacgtg gcagtcggcg tagggctgcg cgcgtcgtcc ggcccgctac aggcggcggc
     7621 gatgagcgcc agcagcagca gcagcgcacc tgcctgccga ttgcctgtcg ttcgcacgcg
     7681 cgtccaccat ccagccatg
//
LOCUS       DASUGJ010000749         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_679367, whole genome shotgun sequence.
ACCESSION   DASUGJ010000749 DASUGJ010000000
VERSION     DASUGJ010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679367"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="VFV93_16960"
     CDS             <1..1979
                     /locus_tag="VFV93_16960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5167101.1"
                     /translation="EGEGTTMAKRPIEAADLFRITLVSDPQVSPDGSQVAYTVTELDE
                     KENRYRAAIWLAPLAGGEPRRLTSGKQRDGQPRWSPDGIRLAFTSDRKQDDDLKGQIW
                     TIPVAGGEPTRLSSLAEGVEEFCWSPDGTALAAVSKVRIGEHNPDSDVRVIRTPRYRF
                     DGEGYLDDKYRQIFGINATSGEARQLTDGAFEHQHPAWSPTGHEIAFDSNRDPGWEML
                     PYRDIYAVRVAGRSIRRVTDSSGEWSTPSWSPDGATLVFYGTRRIMSDAARNELFSVL
                     AGGGAPVALTDHIDRNLRDGATADLVGYPGRAPLWSADGEAVSIVYSEHGQVQLARVS
                     IPDGEMQELTSGPRRVGALSLIPGGGYVYMANTATTPPEIFTCDNDGRNERQLTQHNA
                     AWLGEVEVREPESFWATSEDGRKVHGWIIKPVGFEEGTKYPMVLEIHGGPFGMYGETM
                     MHEFQLLAARGYVVLYTNPRGSTGYGDDFAGALHRGWGKHDMPDLMAAVDWAIGQGYV
                     DEARLGVTGGSYGGFMTNWVIGQTTRFSAAVTGRCISNMYSMFGTDDIFFSSSDQMLG
                     STPWEEPDIYWELSPITYVDRIETPLLIEHQENDHRCPIEQAEQLYTALKRQGKTVEM
                     VRFPDESHGMSRTGQPKHRIERLTYITEWFDRYL"
BASE COUNT          375 a          715 c          697 g          308 t
ORIGIN      
        1 aagagggaga gggaaccacg atggcgaaac gcccgattga agcggcggat ctgtttcgga
       61 tcacgctcgt cagcgacccg caggtgtcgc cggatggatc acaggtcgcc tacacggtga
      121 cggagctgga cgaaaaggag aaccgctacc gggccgcgat ctggctcgcg ccgctggcag
      181 gtggcgagcc gcgccggctg acctccggca agcagcgcga cggccagccg cgctggtcac
      241 ccgacggcat ccggctggcc ttcacctccg accgcaagca ggacgacgac ctgaaggggc
      301 agatctggac gatcccggtc gcgggcggcg agccaacacg cctctcgtcg ctggccgaag
      361 gcgtcgagga gttctgctgg tcgccggatg ggacggcgct cgcggccgtc tcgaaggtgc
      421 ggatcggcga gcacaacccc gactcggacg tgcgcgtcat ccggacaccg cgctaccgct
      481 tcgatggcga aggctacctc gacgacaaat accgccagat cttcggcatc aacgccacct
      541 ctggtgaggc ccgccagctc accgacggcg cgttcgagca ccagcatccc gcctggtcgc
      601 cgaccggcca cgagatcgcc ttcgacagca accgcgatcc gggctgggag atgctgccgt
      661 accgcgacat ctacgccgtt cgcgtcgccg gccggagtat ccgccgcgtc accgacagca
      721 gcggcgagtg gagcacgccc tcctggtcgc cggacggcgc gacgctcgtc ttctatggga
      781 cgcgtcggat catgagcgac gcggcccgca acgagctgtt cagcgtcctg gccggcggcg
      841 gcgcgccggt cgcgctgacc gaccacatcg accgcaacct gcgcgatggc gcgaccgccg
      901 atctggttgg ctaccccggt cgcgcgccgc tctggagtgc agacggtgag gcggtctcca
      961 tcgtctacag cgagcatggt caggtccagc tggcgcgcgt ctcgatcccg gacggcgaga
     1021 tgcaggagct gaccagcggg ccgcgccggg tcggcgcgct ctcgctgatc cccggcggcg
     1081 gctacgtcta catggccaac acggcgacga ccccgcccga gatcttcacc tgcgacaacg
     1141 acggtcgcaa cgagcggcaa ctgacgcagc acaacgccgc ctggctgggt gaggtcgagg
     1201 ttcgcgagcc ggagtcgttc tgggccacca gcgaagacgg ccgcaaggtg catggctgga
     1261 tcatcaagcc ggtcgggttc gaggaaggga cgaagtaccc gatggtcctc gagatccacg
     1321 gcgggccgtt cggcatgtac ggcgagacga tgatgcacga gttccagctg ctggcggccc
     1381 gtggctacgt cgtcctttac accaacccgc gcggcagcac cggctacggc gacgacttcg
     1441 ccggcgcgct ccatcgcggc tggggcaagc acgacatgcc cgacctgatg gcggccgtcg
     1501 attgggccat cggccagggt tacgtcgatg aggcacggct gggcgtcacc ggcggctcct
     1561 acggcggctt catgaccaac tgggtgatcg gacaaacgac ccgcttcagc gccgccgtga
     1621 ccggccgctg catctccaac atgtactcga tgttcggcac cgacgacatc ttcttctcgt
     1681 cgtcggacca gatgctcggc tcaaccccgt gggaggaacc ggacatctac tgggagctct
     1741 cgccgatcac ctacgttgac cggatcgaga cgccgctgct gatcgagcac caggagaacg
     1801 accaccgctg cccgatcgag caggccgaac agctctacac ggccctcaag cgccagggca
     1861 agacggtcga gatggtgcgc ttccccgacg agagccacgg catgtcccgc accggccagc
     1921 cgaagcaccg catcgaacgc ctgacctaca tcaccgagtg gttcgatcgg tacctttgat
     1981 ggtggtgata ggtgttagga gacacacgct ctcgcatctt aagcagcgga ccgatccggt
     2041 aggggcgagg cactgactcg cccttcgtat tccgcccaag cggcgtcggt gggaa
//
LOCUS       DASUGJ010000750         5884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_401761, whole genome shotgun sequence.
ACCESSION   DASUGJ010000750 DASUGJ010000000
VERSION     DASUGJ010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401761"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1018)
                     /locus_tag="VFV93_16965"
     CDS             complement(41..1018)
                     /locus_tag="VFV93_16965"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5167102.1"
                     /translation="MRTDAGLDSDIWRQFYTAEEIDEIVSNSTTTAFVSGTFPIHVRV
                     YAQPGPAPTIVMAHGIICYGLILQRMALPFFRAGYNVVHFDLPGTGQSGGDRGGCTVG
                     EFIRAWGDAVAFARRTFDGPIYAMGNAEDGVTCYYALANHPHVRGISIHNLFHYGDPA
                     GASPIGPSLKVRALTMFSYLGLFLRPSRSVKGTETFPWEHVFTGPGDEKYLKLLEEDP
                     LGLKVVKYRMAHTLLRRRVPRVQFEDCRTPIQVIASKLNAIWPYESTVYYYNRLGGPK
                     ELITLEDKGQWELNPEFHAMYCGHVMHWFAQHGAATGPVDADAHAPVVS"
     gene            complement(1061..4633)
                     /locus_tag="VFV93_16970"
     CDS             complement(1061..4633)
                     /locus_tag="VFV93_16970"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5167103.1"
                     /translation="MTDPFARLGAWMARYRALVLVAWAVIAIVCAVLFAPKTAGVLKA
                     GGIGAPGSESDQADRAAESEFGASVVNNISIVFQSATLTVDDPNYANEVKTVSDRVAA
                     LDGVRSVTSFYSIGDPSLVSADRKTTFVVVALEGDEGATQELVPDVRETLEDTTLDWY
                     VTGLPAINEDFQHTSEQDLQRSEIVTIPLVLILLLVVFRTVISAAIPLFIGAASVLLA
                     LSAIYFIASNADVSVFALNVASMIGLGLGIDFSLIVVSRYREEMARGHDRISATAVTM
                     ATAGRSITYSGVTVVLGMLIMTLMFDLIIIRSIAMGVTIVAATSLLAGLTLLPALMSA
                     LHHRLEWLRVMPKPKPSATEGGFWYRLSHAIMGRPWAWLTVSVILLLVLASPARAMKM
                     IGADPGNLPEDIESVRGARIMEHEYGEARLTPVQVVMRGDGTNSAWTPEFISGLAKLE
                     EAVEADERVQSTTSFPQFMRSPAGGDKNNLPPGTLHELVLYKHGEIRQGPAFMGLATM
                     TLQPGFELPRNATGAIHVIHVESGSLTFQTDGVATISEKPSDPYPPSSPAPIAVPFTL
                     QADQNLIVNEGTPTTIRNDSGQPVELVVGVIYKLQPDEQASATDWTSASNSDLFANVP
                     KNVLSAGIVTNFPEGSVDFLIDRLTFAPHFATPVHEHVGPEIGGVVSGQVEAYGGSAD
                     TSLIIANGQPFPTPFSYATLNPGDSWIHQSGGMHGARNVTDQPTTFIEFRVMASDKAR
                     GPAVMDPPEEGLPVLNIFTPEFFTSNPLSDLVISQFVNLHGNNDTAIINITMKTGQYD
                     DDHEQFIQDLRDEIIPGIPALDAYTVQVGGNSASFQDFRDHLYGRFPWLVGAVLLLTF
                     FILMMFFQSVFLPMKAVFMNLMSILATYGALVLIFQHGYGANLLGFESLNSLSAITPA
                     VLFVILFSLSTDYEVFMLSRVKERYHETHDNEESVAYGLEHTAGIITAAGLILIGTFG
                     SFAVADILTVKEIGIGLAIGVLIDSTIVRVIMVPATMRLAGAANWWMPEWLKRIVPEI
                     KEGPAGEIVPIPRPVPVPVPVAAPALAMSGGGASVASPAMSAAHVPVQVIRRSPSLPV
                     GRLHSTGGSVGAKVITLPRSQAFRIGRDPSSELQVYDVRISRNHAQIEFMDDEFVVRD
                     LGSTNGVVINGQRISSPTVLRNGDRIEFGNMGTVRFTFELTRPS"
     gene            complement(4690..4971)
                     /locus_tag="VFV93_16975"
     CDS             complement(4690..4971)
                     /locus_tag="VFV93_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167104.1"
                     /translation="MSRDEDTGELPVVGNAGDARAPGEPVGRVNLPDETDLQRDPSGL
                     ADDRPAGASAAVAPDSMREKVLFGLMAVVMMILAMIVILQGVFEQKMPI"
     gene            5466..>5884
                     /locus_tag="VFV93_16980"
     CDS             5466..>5884
                     /locus_tag="VFV93_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167105.1"
                     /translation="MNRSRTLMLGIAFVVLLALLAACGGDDTSDATTSTGDQPVAATA
                     TSAEAEATATEPEHAETEDDPTATVADDAASATATEASDDSDDDETATSEPTGDRLVG
                     SASVDDDEDDVVNLLMQEPDDPMPGIDLVKVQIEGDGT"
BASE COUNT         1103 a         1867 c         1903 g         1011 t
ORIGIN      
        1 tgcgatatcg cccctaccgg attggccatt gtgattggtg tcaagagacg accggcgcat
       61 gcgcatccgc gtcaaccggc ccggtcgcgg cgccgtgctg ggcgaaccag tgcatgacgt
      121 ggccgcagta catcgcgtgg aactccggat tgagttccca ttggcccttg tcctccagcg
      181 tgatcaactc cttcgggccg ccgagccggt tgtaatagta gacggtcgat tcgtacggcc
      241 agattgcgtt gagcttcgag gcgatgacct ggattggcgt ccggcaatct tcgaactgga
      301 ccctcggtac ccgccggcgc aacaacgtat gcgccatccg gtacttgacg accttcagcc
      361 ccaacggatc ttcctccagc agcttgaggt acttctcgtc gccggggccg gtgaagacat
      421 gctcccaggg aaaggtctca gttcccttga ctgagcgcga cggccgcagg aacaggccga
      481 ggtagctgaa catcgtcagc gcgcggacct tcagcgaggg gccaattggg ctcgcgccgg
      541 ccgggtcgcc gtagtggaac agattgtgga tcgagatccc gcggacgtgc gggtggttgg
      601 ccagtgcgta gtagcaggtg acgccgtctt cggcattccc catcgcgtag atcggtccat
      661 cgaaggtacg gcgggcgaag gcaactgcat cgccccaggc acggatgaac tcaccgaccg
      721 tgcagccgcc gcgatcgccg ccgctctggc cggtgcccgg caagtcgaag tggacgacgt
      781 tatagccggc gcggaagaac ggcagcgcca tccgttgcag gatcagccca tagcagatga
      841 tcccatgcgc catcacgatc gttggcgccg gccccggctg ggcgtagacg cggacgtgga
      901 tcgggaacgt cccggagacg aacgccgtcg tcgttgagtt ggagacgatc tcgtcaatct
      961 cctcggctgt gtagaactgt cgccagatgt cactgtcgag accggcatcg gtgcgcattt
     1021 ggtggcctcc agctttttcc atcagactca tccacgaacg ctaggacggg cgcgtcaact
     1081 cgaacgtgaa ccggacggtg cccatgttgc cgaactcgat cctgtcccca ttgcggagaa
     1141 cggtcgggct gctgatgcgc tgcccgttga tgacgacgcc gttcgtgctg ccgaggtcgc
     1201 ggacaacgaa ctcgtcgtcc atgaactcga tctgggcgtg attgcgcgag atgcgtacat
     1261 cgtagacctg cagctcggaa ctgggatcgc ggccgattcg gaacgcctgc gagcgcggca
     1321 gcgtgatgac cttcgcgccg accgagccgc cggtcgagtg cagccgtcca accggcagtg
     1381 acggcgagcg ccggatgacc tggaccggta catgcgcggc gctcatggcc gggcttgcaa
     1441 cggacgcccc gccacctgac atcgccagcg ccggtgcggc tacaggaacc gggacaggaa
     1501 ccggacgcgg gatcgggacg atctcaccag caggtccctc cttgatctcc gggacgatcc
     1561 gcttcagcca ctccggcatc caccagttcg cggcgcccgc cagccgcatc gtggcaggaa
     1621 ccatgatgac acgcacaatc gtcgaatcga tcaggacacc gatcgccagc ccgatcccga
     1681 tctccttgac agtcaggatg tcggcgacgg caaacgaccc gaaggtgccg atcaggatta
     1741 gtccggcggc cgtgatgatc ccggccgtgt gctccagccc gtaggcgacc gactcctcat
     1801 tgtcgtgcgt ctcgtggtag cgctccttga cccgcgagag catgaagacc tcgtagtcgg
     1861 tcgagaggct gaagaggatc acgaacagga cggccggagt gatcgcgctg agactgttca
     1921 gcgactcgaa gccgagcagg ttcgcgccgt agccatgctg gaagatcagc acaagtgccc
     1981 cataggtcgc caggatcgac atcaggttca tgaagacggc cttcatcggc aggaagaccg
     2041 actggaagaa catcatcagg atgaagaacg tcagcagcag gacggcgccg accagccacg
     2101 ggaatcggcc atacaggtgg tcgcggaagt cctggaagct ggccgagttc ccgccgacct
     2161 ggaccgtgta ggcatcgagg gccgggatgc cggggatgat ctcgtcgcgt agatcctgga
     2221 tgaactgctc gtgatcgtcg tcgtactgac ccgtcttcat cgtgatattg atgatggccg
     2281 tgtcgttgtt gccgtgcagg ttgacgaact gcgagatgac gaggtccgaa agtgggttgg
     2341 acgtgaagaa ctcgggcgtg aagatgttca gaaccggcag gccctcctcc ggcgggtcca
     2401 tcacggccgg tccacgtgcc ttgtccgacg ccatcacgcg gaactcgatg aaggtcgtcg
     2461 gctgatcggt gacgttgcgt gcgccgtgca tcccgccgct ttggtggatc cacgagtctc
     2521 ctggattgag cgtcgcatac gagaacggcg tcgggaatgg ctggccgttg gcgatgatca
     2581 ggctcgtgtc ggctgagccg ccgtacgctt ctacctgacc ggagacgacc ccgccgatct
     2641 ccggaccgac gtgctcgtgg accggtgtcg cgaagtgcgg cgcgaacgtc agccggtcga
     2701 tcaggaagtc gaccgagcct tcggggaagt tggtgacgat accggccgag agcacgttct
     2761 tcggcacgtt ggcaaacagg tcgctgttcg acgcgctcgt ccagtcagtt gcggacgcct
     2821 gttcgtcggg ttgcagctta tagatcacgc caaccacaag ctccactggc tggccgctgt
     2881 cattgcggat cgtcgtcggc gtcccctcgt tgacgatcag gttctggtct gcctgaagcg
     2941 tgaacggcac ggcgatcggc gccggtgacg acggcggata cgggtcggac ggcttctcgg
     3001 agatggtcgc aacgccgtcg gtctgaaacg tcagcgagcc ggattcaaca tggataacat
     3061 ggatggcgcc ggttgcgttg cgcggcagct cgaagccggg ctggagcgtc atcgtggcga
     3121 ggcccatgaa ggccggtccc tgccggatct cgccgtgctt gtagagcacc agctcgtgca
     3181 gcgtgccggg cgggaggttg ttcttgtcac caccggccgg gctgcgcatg aactgtggga
     3241 acgatgtcgt cgactggacc cgctcgtcgg cctcaaccgc ctcctccagc ttcgccaggc
     3301 cggagatgaa ttcgggtgtc caggcgctgt tggtgccatc gccgcgcatg acgacctgga
     3361 ccggcgtcag ccgcgcttcg ccgtactcgt gctccatgat ccgggcgccg cgcaccgatt
     3421 cgatgtcctc cggcaggttg ccgggatcgg cgccgatcat cttcatcgcg cgggccggcg
     3481 acgccagcac cagcagcagg atgaccgaca ccgtcagcca ggcccacggc cggcccatga
     3541 tcgcgtggct gagccggtac cagaagccgc cttcggtcgc gctgggcttc ggcttcggca
     3601 tcacccgcaa ccactccagc cggtgatgga gggccgacat cagcgccggc agcagcgtca
     3661 ggccggccag cagcgaggtt gcggccacga tcgtgacgcc catcgcgatc gagcggatga
     3721 tgatcagatc gaacatcagc gtcatgatca gcatgccgag tacgaccgtg acgccggaat
     3781 aggtaatcga gcgtccggcc gtcgccatcg tgacggccgt cgccgagatg cggtcgtggc
     3841 ccctggccat ctcctcgcgg tagcggctga cgacgatcag cgagaagtcg atcccgaggc
     3901 cgaggccgat catcgaggcc acgttcagcg cgaagaccga aacgtcggca ttcgaggcga
     3961 tgaagtagat cgcgctgagc gccagcagga cgctggccgc gccgatgaag agcggtatcg
     4021 cggccgagat gaccgtccgg aagacgacca gcagcaggat cagcacgagc gggatcgtca
     4081 cgatctccga gcgttgcagg tcctgctcgc tcgtgtgctg gaagtcctcg ttgatggccg
     4141 gcaggccggt cacgtaccag tcgagggtcg tgtcctccag cgtctcgcga acgtcgggaa
     4201 ccagctcctg ggtcgcgccc tcgtctccct ccagcgcaac aacgacgaag gtcgtcttcc
     4261 ggtcggcgct gaccagcgag ggatcgccga tcgagtagaa ggacgtgacg ctacggacgc
     4321 cgtcgagcgc cgcgacgcgg tcgctgaccg tcttaacttc gttggcgtag ttggggtcgt
     4381 caaccgtcaa cgtcgccgac tggaagacga tcgaaatgtt gttgacgacc gatgcgccga
     4441 actcactctc ggcggcacga tcggcctggt ccgactcgga gccgggcgcg ccgattccgc
     4501 cggccttcag gacaccggcc gtcttgggcg cgaagagcac tgcgcagacg atggcgatca
     4561 ccgcccaggc aaccagcacc aacgctcggt agcgcgccat ccatgcgcca agccgggcaa
     4621 atgggtcagt catccacgat gctcctcacg ccgccacggg accacgttcc cgcaccggct
     4681 ctgacatgct cagatgggca tcttctgctc gaacactccc tggaggatca cgatcatcgc
     4741 caggatcatc atcacgaccg ccatcaggcc gaacagcact ttctcgcgca tggagtctgg
     4801 agccacggcg gcgctcgccc cggcaggccg gtcatcagcc agtccggagg gatcacgttg
     4861 cagatcggtc tcatcaggca ggttgacacg cccaacgggt tcgccaggcg cccgcgcgtc
     4921 tccagcgttg ccgacaacag gcagctcgcc cgtgtcctca tccctgctca cagcaaccac
     4981 caatcctgag gcgacgtcgg gtgtcctgac cgtggcattg tggaccggtt tccggcatgc
     5041 tcccccgctc gcgcacgagc agcgaaacat gtccggttca gggatcgtct tctattctga
     5101 gagccggcgg cgctactgcg tcatctgccg tcagactcac gtactccggc aattactgaa
     5161 gagcgccctc cacaagggca ggagggcgtt acgccggcga ggcagcgtgc tcgaccatcg
     5221 accgtcaacg gcagatcgcg gtcgttcctg cgtccctcgt ggcgtgggcg catcctacgc
     5281 cagtcgttgc cggcggtcaa ctcgcaaccg gcactaacta cgctatcggg aagtgccgga
     5341 tggggctatc gcattcgcaa ccaaacgact gtaaagcttg atacattctc aactcccgcg
     5401 atcatcgata ctggcattgc acaagcgagc atgatgggaa tgacatcaat ctgaaaggcc
     5461 ggggcatgaa ccgatcacga acgctcatgc tggggatcgc cttcgtcgtc ctgctggcac
     5521 tgctggcagc ctgcggcgga gacgacacgt ccgatgcgac gacatcgacc ggcgaccagc
     5581 cggtagccgc gaccgctacg tcggctgagg cagaagcaac agcgaccgag ccggagcacg
     5641 ccgagaccga agacgacccg accgcgacgg tggccgatga cgccgccagc gcgacagcga
     5701 ctgaggcttc cgacgacagt gacgacgacg aaacagccac gtcggaacca acaggtgacc
     5761 ggcttgtcgg cagcgcgtcc gtcgatgacg atgaagacga tgttgtcaat ctgctgatgc
     5821 aggagccgga cgatccgatg ccgggtatcg atctcgtcaa ggtccagatc gaaggcgatg
     5881 gcac
//
LOCUS       DASUGJ010000751         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_401781, whole genome shotgun sequence.
ACCESSION   DASUGJ010000751 DASUGJ010000000
VERSION     DASUGJ010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401781"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..454
                     /locus_tag="VFV93_16985"
     CDS             134..454
                     /locus_tag="VFV93_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167106.1"
                     /translation="MTPTLWVGIIVIVCILEFVRIGMTTYVLVHLLESHSLRRQGEES
                     GAARRSLQSASARARREFLLDPTLTGLRAAMDEVAVGRAGPGVQRAIEALDRYALAVV
                     DERR"
     gene            459..752
                     /locus_tag="VFV93_16990"
     CDS             459..752
                     /locus_tag="VFV93_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167107.1"
                     /translation="MSAFFVLYALTISIELVLSLVMAIFLFLILAPVRSHQPDQPQAT
                     PQARPLARTVEGALGDAFATVSEGRDDLATLTNARTLLQQYQRERFDAARHAS"
     gene            1610..>2853
                     /locus_tag="VFV93_16995"
     CDS             1610..>2853
                     /locus_tag="VFV93_16995"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX5167108.1"
                     /translation="MSEIERLVEAGATLDGNGDDESEITISTFIRVRPGREEDFEDYL
                     RGFNATAAEFPGYIGARLFRPARGDNRYRIVLRFDSERNLTHWNESEERQIWAERGEE
                     FTEAPPWSTNITGTAQASPLALARTPLNQYVQSNISGIGLLLLGTVLALIFANSPLSD
                     TYANFWNTKLTIGTDRFAIEETLRHWVNDGLMALFFFIVGLEIKREVLVGELRRPRQA
                     ALPIAAAVGGAIGPALVYTLIVFGGHGSQGWGIPIGTDTAFSIGIIALLGSRVHPQLL
                     VFLTAFAIIDDIIAVMVIAIFYTDQIVWSGVVAAIVLFLILIVVNRAGVHRWPVYAFL
                     GVALWLAVFESGIHGTLAGVLVAMVVPSRAWINPSEFLARGRRLMTDFERASYSTLNM
                     LSNEPQQQATQSLARLTEQVEAP"
BASE COUNT          487 a          971 c          908 g          487 t
ORIGIN      
        1 gctcctcgtt cgtggaccgg acgatgttca gcccgctcat gttgacgcag aatcgctcga
       61 cgacgacatc tgggttctca ccgcagtccc ggaagctgcc ggggcgtccc caccaatgga
      121 ctgaacctgc tccatgacac ctacactttg ggtcggaatc atcgttatcg tctgcatcct
      181 ggagttcgtg cggatcggga tgacgaccta cgtcctcgtg cacctgctgg agagccactc
      241 actgcggcga cagggcgagg agtcgggcgc ggcgcggcgc tcgttgcagt ccgcatctgc
      301 cagggcgcga cgcgagttcc tgctcgaccc gaccctgacg gggttgcgag ccgcgatgga
      361 cgaggtcgcc gttggccggg ccgggccggg tgtccagcgg gcgatcgagg cactggatcg
      421 ctacgcgctg gcggtggtcg atgaacggcg atagcgccat gagcgccttc ttcgtcctct
      481 atgcgctgac gatcagcatc gagctggtcc tcagcctcgt catggcgatc tttctgttcc
      541 tgatcctcgc gccggtccgc agccaccagc ccgatcagcc gcaagccacg ccacaggcgc
      601 gaccgctggc ccgcaccgtc gagggcgccc tcggcgacgc cttcgcgacc gtctccgagg
      661 gccgcgacga cctcgcgacc ctgaccaacg cgcgcacgct cctccagcag taccagcgcg
      721 aacggttcga cgcagcccgc cacgcgtcgt aaccgacgca cacgaacact cacgggagat
      781 cccacccgca gtagtgttcg gcgcttgggc cgcctgtcac gtaaaataac gattggaacg
      841 atggtgctgt tgccatcccg ccctgctccc ggacgatgcc gatcagtggc atgaacatca
      901 gcaacgaacc cggtcatcgt ccggccggat tccgtaacgc cgtgggcggc ggttccctcg
      961 ggcagcgggt gcgccatcct cgttggtggt cccggcacgc gtcacccgcc ggctgtgttc
     1021 ggccggcaac cagttccatg accagcaggc cgttggcaat cggccacggg cgctgctggc
     1081 cgttatcagg gaacgtgact tcaatccgcg ctgtgtcgat ggaccggacg gtgcctcaac
     1141 cgccaggggt ttcggcacag gcaacggaca ggccggccgt cagcgccggc ccgacccgcg
     1201 cggctggggc aataccaggc agggatgcca gcgatgaacg caccccgacg acaaccagac
     1261 cccggcccat ccactccccg attgtcgacc cgcccggatc aatgcccgac cggcagcact
     1321 gcgagctggg cgaaccggcg agcggccgga taccactcgt acgagaagac tcggaaaagc
     1381 cggctggacg gagcggagca gtctccaccg caatcggcac aactgccacg gcggcgttcg
     1441 cggcacgcgt cttgtcgtat cggtcgcgtc gtggacgacg agccgccatc cgaccggcga
     1501 gtgaccgttt gtgcgcgaca agggatatgg aaagagagac ccgttgacac gcgaaaacga
     1561 gcgagggagt ccatcggatt cggttgacac cggccctgcc gggcgcgaga tgagcgaaat
     1621 cgaacggctg gtcgaagccg gcgcgacgct ggacggcaac ggcgacgacg agtcggaaat
     1681 cacgatcagc acgttcatcc gcgtgcggcc cggccgggaa gaagacttcg aggattacct
     1741 gcgtggcttc aacgccacgg ccgccgagtt ccccggctac attggagcgc gtctcttccg
     1801 gcccgcccgt ggagacaatc gctaccggat cgtgttgcgc ttcgacagcg aacgcaatct
     1861 cacccactgg aacgagtccg aggaacgtca gatctgggcc gagcgcggtg aggagtttac
     1921 cgaagcaccg ccgtggtcga ccaacatcac cgggaccgcc caggcaagcc cgctcgcgct
     1981 ggcgcggacg ccgctcaacc agtacgtcca gtccaacatc tccggtatcg gcctgcttct
     2041 gctcggcacg gtgctggcgc tcatcttcgc caactcgccg ctctccgaca cctatgctaa
     2101 tttctggaat accaagctga cgatcggcac cgatcgtttc gcgatcgaag agaccctgcg
     2161 gcactgggtc aacgatggcc tgatggcgct cttcttcttc atcgtcggcc tggagatcaa
     2221 gcgcgaggtg ctggtcggcg agctacgccg cccccggcag gcggcactcc cgatcgcggc
     2281 ggccgtcggt ggcgcgatcg gcccggcgct ggtctatacg ctgatcgtct ttggcgggca
     2341 cggcagtcag ggctggggca tcccaatcgg gaccgacacg gcgttctcga tcggcatcat
     2401 cgccctgctc ggcagccggg ttcacccgca gctcctcgtc ttcctgacgg ccttcgcgat
     2461 catcgacgac atcatcgcgg tgatggtaat cgctatcttc tacaccgacc agatcgtctg
     2521 gagcggggtt gttgccgcga ttgtgttgtt tctgatcctg atcgtcgtca accgggccgg
     2581 cgttcatcgc tggccggtct acgcattcct cggcgtcgcg ctctggctgg cggtcttcga
     2641 atccggcatt cacgggacac tggccggtgt cctggtggcg atggtcgtcc cgagccgggc
     2701 ctggatcaat cccagcgaat tcctggcccg tggccggcgg ctgatgaccg acttcgagcg
     2761 cgcctcgtat tcgacgctca acatgctctc caatgagccg cagcagcagg cgacccagtc
     2821 gctggcgcgg ctgaccgagc aggtggaagc gcc
//
LOCUS       DASUGJ010000752         5054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_679778, whole genome shotgun sequence.
ACCESSION   DASUGJ010000752 DASUGJ010000000
VERSION     DASUGJ010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679778"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1357)
                     /gene="glp"
                     /locus_tag="VFV93_17000"
     CDS             complement(95..1357)
                     /gene="glp"
                     /locus_tag="VFV93_17000"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX5167109.1"
                     /translation="MGNERAWDTPEAMLSVDDALARILDRFSPLPAVETAILDALGMV
                     LSADVVSTVDVPPFRNSAMDGYAIRAEDTASAPVALSVVGEVAAGNVADRPVGRGEAI
                     RIMTGAPVPDGADSVVRFEETDESARSGRSETPAIGISRQIAVGENVRLAGEDLRSGQ
                     IALQAGLRVGPAEIGVLASLNLAAVAVHRRPRVAILSTGDELVEPGSDLRPGQIRDSN
                     GVMLAAYVRQAGGEPILAGIAGDSTEDLRSKLRAFSAPDLFITSGGVSVGDYDVVKDV
                     LRAEGEIDIWQVRMKPGKPLAFGTIADVPLLGLPGNPVAALVSFLQFGRPAIRKMLGH
                     RNLCLPEVDATLAAELRNRGRRRHFVRGVVATTPSGFEAHPVAATGSAILSAASRANC
                     FIVVPEMCDVVEAGETVRVQLIDDVGLV"
     gene            complement(1392..2321)
                     /gene="mdh"
                     /locus_tag="VFV93_17005"
     CDS             complement(1392..2321)
                     /gene="mdh"
                     /locus_tag="VFV93_17005"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX5167110.1"
                     /translation="MRGKVTVVGAGNVGSVVAQKLAERDYADVVLLDIVEGMPQGKSL
                     DLMQSGPVVGYDTNLTGANAYEDTAGSDVVVITSGIARKPGMSRDDLLRTNMGIVKSV
                     TEQVVAASPDTVIIVVSNPLDAMCHAAFDAAGFPKERVIGMAGVLDSARLRTFVAMEL
                     DVSVEDVQAMVLGGHGDTMVPMPRYTTVGGIPITELLSAERIAAISERTANGGAEIVG
                     LLKTGSAYYAPGNSATQMVDAIMLDKNRVVPASVLLEGEYGINGLYVGVPVKLGAGGV
                     KQIIEVLLTDAEAAALHKSAGAVQGLVDAMKAL"
     gene            2535..2621
                     /locus_tag="VFV93_17010"
     tRNA            2535..2621
                     /locus_tag="VFV93_17010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2569..2571,aa:Leu,seq:gag)
     gene            2636..2711
                     /locus_tag="VFV93_17015"
     tRNA            2636..2711
                     /locus_tag="VFV93_17015"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2669..2671,aa:Val,seq:tac)
     gene            2741..2816
                     /locus_tag="VFV93_17020"
     tRNA            2741..2816
                     /locus_tag="VFV93_17020"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2774..2776,aa:Phe,seq:gaa)
     gene            2962..3396
                     /locus_tag="VFV93_17025"
     CDS             2962..3396
                     /locus_tag="VFV93_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167111.1"
                     /translation="MLDRRTVMLVNNEPTFRWTVSRLLFDSGYAVDAVERSPAAVEHA
                     RDIRPGAVVIEASYGSALSATSIVERLRTTPETRYLPIIVCSPDGRFLHSYGEYLRGQ
                     GCVVIGRPFNSERFVELVQRAVAPSPALASADFPASTSAATW"
     gene            3525..>5054
                     /locus_tag="VFV93_17030"
     CDS             3525..>5054
                     /locus_tag="VFV93_17030"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX5167112.1"
                     /translation="MSDVGLGLTAALVAAGAAILFGLFLARSVLSRPAGNERMQDIAR
                     AIQEGAEAYMKRQYTVVAMVAVVIAVLLAVALNVETAIGFLVGATASAAAGFIGMSVA
                     VRANVRTAEAGRSGLGPALTTAFQGGTVTGLFVVGLGLASVAGFYAITGNVDSLIGLG
                     FGGSLISVFARIGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDCA
                     GMAADLFETYAVTAIAAMLLGDLVFPGNEKAIVFPLVLGAVSIIASIIGTFFVRTGSG
                     GSIMGALYKGLFGAAALSVIAFYPVTQWLMDDNGIIGDQDVSLFGIEFGMGPTLKLFL
                     CALLGIVVTMAIVFITEYFTSTSFKPVRNIARASETGHGTNIISGLAVSMQATAPVIG
                     VIALAIYLAYNIATGSDPHVALYGIAVAAMAMLSMAGIVVAIDSFGPITDNAGGIAEM
                     SEMPDEVRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFASFFLELEEQIPFDLTN
                     PN"
BASE COUNT          854 a         1696 c         1614 g          890 t
ORIGIN      
        1 cctcaatgca atgaaccaat acgcggattg gacgattgag attgagggcg agtcaccgac
       61 atcgccccta cgcattgatt gtgcgattgg ttcttcagac gagtccaacg tcatcgatca
      121 gttgcacgcg aacggtctcg ccggcctcaa ccacgtcgca catctcgggg acgacgatga
      181 agcagttggc gcgcgacgcc gccgacagga ttgcggagcc ggttgccgcg acggggtgtg
      241 cttcgaatcc cgatggcgtc gtcgcgacga caccgcgaac gaagtgccgc cgccggccgc
      301 gattgcgaag ctctgcggcg agcgtggcgt cgacctccgg caggcagaga ttgcgatggc
      361 ccagcatctt gcggatggct ggccggccga actggaggaa cgagaccagc gcggcgaccg
      421 ggttgccggg caggccgagc agcgggacgt cggcgatcgt gccgaacgcg agcggcttgc
      481 ccggtttcat ccgcacctgc cagatgtcga tctccccttc ggcccgcagg acgtccttga
      541 cgacatcgta gtcaccgacc gagacgccgc cggaggtgat gaacaggtcc ggcgcggaga
      601 atgcgcgtag cttcgaacgc aggtcctcgg tcgaatcacc ggcgatcccg gccaggatcg
      661 gttctccgcc tgcctgtcgg acgtaggcgg ccagcatgac accattgctg tcgcggatct
      721 ggcccggtcg caggtctgaa cccggttcga ccagttcatc cccggtcgag aggatcgcga
      781 cgcggggacg gcgatggacg gcgaccgccg caaggttcag cgaggcgagg acaccgattt
      841 cggccggccc cactctcaaa ccggcctgca acgcgatctg gccggatcgg agatcctcac
      901 cggccaggcg tacgttctcg ccaaccgcga tctggcggct gatgccgatc gcaggagtct
      961 ccgaccgtcc cgatcgcgca gattcgtcgg tctcctcgaa acgaacgacc gagtcggctc
     1021 catcgggtac gggcgcgcct gtcatgatcc ggatcgcctc gccgcgaccg accggccgat
     1081 ccgcgacgtt gccggccgcg acttcgccga cgactgacag cgccaccggc gcggaggccg
     1141 tgtcttcggc gcggatcgcg tagccgtcca tcgcggagtt gcgaaacggc gggacatcga
     1201 cagtcgagac gacatctgcg ctgagaacca tcccgagcgc gtcgagaatc gccgtctcga
     1261 cagccggaag cggcgagaac cggtcgagga tccgcgccag cgcgtcatcg acgctcagca
     1321 tcgcttccgg agtgtcccag gcgcgttcgt tacccatgtc tcctgacagt cgttgacgcg
     1381 aacaggcggc gctagagcgc cttcatcgca tcaaccaggc cctgaacggc gccggccgac
     1441 ttgtgcaacg cagccgcctc ggcgtcagtc agcaagacct cgatgatctg cttgacgcca
     1501 ccggcgccga gcttaaccgg cacgccgacg tagaggccat tgatgccgta ctcgccctcc
     1561 agcaggacgc tggccggcac aacccggttc ttgtcgagca tgatcgcgtc aaccatctgc
     1621 gttgccgagt tgcccggcgc gtagtaggcc gagccggtct tcagcaggcc gacgatctcg
     1681 gcgccgccgt tggcggtccg ctcggagatg gccgcgatcc gctcggctga gagcagttcg
     1741 gtgatcggaa tcccgccaac cgtcgtgtag cgcggcatcg ggaccatcgt gtcgccatgg
     1801 ccgccgagaa ccatcgcctg gacgtcttcg accgagacat ccagctccat cgccacgaac
     1861 gtccgcaagc gggccgagtc gaggacaccg gccatcccaa tcacgcgctc cttcgggaag
     1921 ccggccgcgt caaaggcggc gtggcacatc gcatccagcg ggttcgagac gacgatgatg
     1981 accgtgtccg gcgaggcggc gacgacctgc tcggtaacgc tcttgacgat ccccatgttg
     2041 gtgcgcagca ggtcgtcgcg gctcatacct ggcttgcggg cgatgccgct ggtgatgacg
     2101 acgacgtccg aaccggccgt gtcctcgtag gcgttcgcgc cggtcaggtt cgtgtcgtag
     2161 ccgacaaccg ggccagattg catcaggtcg agcgacttgc cctgcggcat cccctcgacg
     2221 atgtcgagca ggacgacatc tgcatagtcc cgttcggcga gcttctgcgc gacgacacta
     2281 ccgacatttc ccgcgccgac gacggttact ttgcctcgca ttgttggtcg ttcctccctc
     2341 gtccccactg tgccgcgagc ggcttcgggc cgcccattcg agcggcaggt gttcgattca
     2401 tgatagcatc gggacagcgg caggcaggca gtgaagacca cgccggctgc cggaccaagc
     2461 catcgccgga gtgcttgatt ccggtcccga gccgggatat acttcattgc gctcaagcgg
     2521 aggctttgag gcttgcccag gtggcggaat aggcagacgc gctagcttga ggggctagtg
     2581 cccgcaaggg cgtgggggtt caagtccctt cctgggcacc aaacggcacg tgcaagggcg
     2641 attaactcag ctggtagagt gccacgttta caccgtggat gtcagaggtt cgaaccctct
     2701 atcgcccacc atcgcacggt gctgataccc accacctcat gccgaggtag ctcagtcggt
     2761 agagcatacg actgaaaatc gtagtgtcgg cggttcgatc ccgcccctcg gcaccaccag
     2821 acgggacgga gcagccaggc tccgtcccgt tcgcgtttcg gccaatccgc acgccagcac
     2881 tgattctctg tgactgaatg gcacgctccc tgcgtagtta ccgtacgtac gcgggatcgc
     2941 gcgttggatt gtgaggaatc gatgctggat agacggacag tgatgctggt caacaacgag
     3001 ccgaccttcc gctggaccgt gtcgcggctg ttgttcgaca gtggctacgc cgtcgatgcc
     3061 gtcgagcgca gcccggcggc ggtcgagcat gcccgcgaca tccgccccgg cgcggtcgtg
     3121 atcgaggcga gctacgggag cgccctatcg gcgacgagca tcgtcgaacg cctgcgaact
     3181 acgccggaga cgcgctacct gccgatcatc gtctgctcgc cagatggcag gttcctgcac
     3241 tcgtatggag aatatctgcg cgggcagggc tgcgtcgtca tcggccggcc cttcaacagc
     3301 gagcgcttcg tcgagctggt acagcgcgca gttgccccgt cgccagcgct tgcctccgct
     3361 gatttcccgg cttctacatc cgccgcaacc tggtaacgaa ccgacacctg acggcccact
     3421 ccgcgagtca aaactgacgg acccacgctc gaatcggtat actgccgact gcccgcacgc
     3481 aggggggcgg gtgtttctgt gcacaatcag ggggatggga atctgtgtca gacgtagggc
     3541 tgggtctcac cgcggcgctc gttgccgcag gggcagcgat cctgttcggt ctgttcctgg
     3601 cacgctccgt cctcagccgt ccggctggga acgagcgcat gcaggacatc gcgcgcgcca
     3661 ttcaggaggg cgcggaagcc tacatgaagc ggcagtacac ggtcgtcgcg atggtggcgg
     3721 tcgtgatcgc tgtcctgctg gccgtcgcgc tcaacgttga gacggccatt ggcttcctgg
     3781 tcggcgcgac cgcgtcggca gcggccggct tcatcggcat gagcgtggcg gttcgcgcca
     3841 acgtccgaac ggctgaagcg ggacgttccg gcctcggtcc ggcgctgacg accgccttcc
     3901 agggcggcac cgtcaccggc ctgttcgtcg tcgggctcgg cctcgcctcc gtcgccggct
     3961 tctatgcgat caccggcaat gtcgactcgc tgatcggtct cggcttcggt ggctcgctga
     4021 tctcggtctt cgcccggatc ggcggcggca tctacaccaa ggccgccgat gtcggcgccg
     4081 acctggtcgg caaggtcgaa gccggcattc cggaagacga cccgcgcaac ccggccgtca
     4141 tcgccgacaa cgtcggtgac aacgtcggcg actgcgccgg catggcggcc gacctgttcg
     4201 agacctacgc cgtcaccgcc atcgccgcga tgttgctcgg cgatctcgtc ttcccaggca
     4261 acgagaaggc aattgtcttc ccgctcgtgc tcggcgccgt ctcgatcatc gcgtcaatca
     4321 tcggcacgtt cttcgtccga accggttccg gcggctcgat catgggcgcg ctctacaagg
     4381 ggctcttcgg cgcggccgcc ctctcggtca tcgccttcta ccccgtcacg cagtggctga
     4441 tggacgacaa cggcatcatc ggtgaccagg atgtcagcct gttcggcatc gagttcggaa
     4501 tgggcccgac cctgaagctc ttcctctgcg ccctgctggg aatcgtcgtg acgatggcga
     4561 tcgtcttcat caccgagtac ttcacctcaa ccagcttcaa gccggtacgg aacatcgctc
     4621 gtgcgtcgga gacgggccac ggcaccaaca tcatctccgg ccttgctgtc tcaatgcagg
     4681 cgaccgcgcc ggtcatcggc gtgatcgcgc tggcgatcta cctcgcctac aacatcgcca
     4741 ccggaagtga cccgcacgtc gccctctacg ggatcgcggt tgcggcgatg gcgatgctct
     4801 cgatggccgg gatcgtcgtc gcgatcgact cgttcggccc gatcaccgac aacgccggcg
     4861 gcatcgccga gatgtcggag atgccggacg aggtgcgcgc gatcaccgat ccactcgacg
     4921 ccgtcggcaa cacgaccaag gccgtcacca agggctacgc gatcggctcg gccggtctgg
     4981 ccgcgctggt tctcttcgcg tcgttcttcc tggagctgga agagcagatc ccgttcgacc
     5041 tcaccaaccc gaat
//
LOCUS       DASUGJ010000753         6459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1929362, whole genome shotgun sequence.
ACCESSION   DASUGJ010000753 DASUGJ010000000
VERSION     DASUGJ010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929362"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /gene="menA"
                     /locus_tag="VFV93_17035"
     CDS             <1..1758
                     /gene="menA"
                     /locus_tag="VFV93_17035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HEX5167113.1"
                     /translation="GVRVALPADLVAAARRGGSCRAGAGLMASNRRWLALLLALSGPI
                     VVLAIWEALSRAEAINPLFFPAPTSLLPTTRDLIESGQLWRDTRISMGRVLTGFAVAA
                     VPGVLIGIMMALWWPLRAMLTPIAAAFFAIPKIAVLPLVIIIFGIGETSKVAMVAVSV
                     LFLVVLSTMSAVLEVERAYFDVARNAGANWWQQLRTVALPGALPGIFSGLRIALGFSL
                     LVIVGTEMLAAKDGIGYLIWNSYQTFAIEKMYVGLVVTALLGWLLNLVMDEIERVVIP
                     WRAPESRGISLPVPEKMRNWFMATRPFSFTASAVPVLLGTLLAAHDGEFDLLLFALVI
                     VASVIVHAASNLVNDYFDWKNRADKPAALGRGGYIQRGIIRPEGILWFGLALFGVATA
                     IGLVIVYLVGWPVLLFALPSLAAAYFYTGGPKPLGYVALGEATVFLFMGPVIVVGSYY
                     VQTGAIAWAPVLLSVPVGLLVTAILQANNIRDIADDTEAGKRTLATYIGRRWANREYV
                     LLLFGSYLVIGLIALGGVIPAGALIVFLTLPRAIEVTRIVLHRDEARFLNAALRQTAG
                     IHLQFGTILSAVLLATVVF"
     gene            complement(1755..2588)
                     /locus_tag="VFV93_17040"
     CDS             complement(1755..2588)
                     /locus_tag="VFV93_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5167114.1"
                     /translation="MERLDWFEVVRFPQGVTMIAEPGHSEDVKSYLVEGERDVAILDT
                     GMGVGDFAGLVATLSDRAPIVLHSHAHFDHVGASNRFDRVLVHASEADDLREGVPNER
                     FRRWFEPQHLVGDRLPVDFDPEQARIEPAEPTGAIEPGQRFDLGGRVLEAFHTPGHSP
                     GGITLLDRENRLLFPGDAVYTGPMFAWRPYSDPIAYRESLRLLAELADLVDVVYPSHN
                     RVPLMPDEVRSMHYAYEEIWSGRVPDQRDADKDTFDFGDFSFWLRRDGYGPWLAGESQ
                     G"
     gene            2754..3845
                     /locus_tag="VFV93_17045"
     CDS             2754..3845
                     /locus_tag="VFV93_17045"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HEX5167115.1"
                     /translation="MSETMTTPTPMFDLIKTLTEIPGPTGQEDLVHEWCADHWSSFAE
                     ETLITRVGNVAARVGGDGPAIVVLAHGDELGLMIKSITDNGLLHVWPIGRDPRGRPSH
                     SYAPVNQPVIVLADSGQVEGQLVYASGHVIGGGNQKDHFEWNDWFVDLGYTSREKVES
                     FGIHPGTRIALNPPTRRLGDTIVGKAMDNRAALAIATSVGERADTAKLKYQLWIASSV
                     QEENGLLGASSIPEIADFSYGIALDVGLCGDVPGTSSENHPSKLGNGPILVHQDSSVH
                     YSHRMTMALAETAATAGIPVQHAIFQNYGSDGAELIRRGIESVLLTMPTRYTHSPNEM
                     ITETECVQCADLILAWLEQEPLPPRWTKA"
     gene            complement(3861..4190)
                     /locus_tag="VFV93_17050"
     CDS             complement(3861..4190)
                     /locus_tag="VFV93_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167116.1"
                     /translation="MPDRPRHKRRRRLRGGYPETMFSRYLRSPYDPDNSRNMWGYIVG
                     RTLANPPRRGAGRWFAWIQALVLCASGIAGITFGLDGDLLPLAFGILFGIAGIVVLSR
                     LIGSRRH"
     gene            complement(4276..6213)
                     /locus_tag="VFV93_17055"
     CDS             complement(4276..6213)
                     /locus_tag="VFV93_17055"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5167117.1"
                     /translation="MGGRGGGRIGGPAPDDRPKRTRAEDGRTLSRVARIFRPYRKHVF
                     FVLLAIVATSIIGIANPILLKLIIDDAIPSGDMSLLTLYVGLMILAPIVGGLIGVGQT
                     YFSNLIGQRVMRDIRMRLYGHLQGMSMRFFTTTRTGEIQSRLSNDVGGVQGVVTNTAG
                     SVVSNLTTVISTVVAMVVISVELTALSFALMPIALYLTYKVGQKRRIIANETQRSMAD
                     MTSMVEETLSVSGVLLTKTFGRQSYEIGRFDDENERLAALQIRQQMIGRWFFMIVGTF
                     FSIAPALAYWLAGRSIIQADNAGQTAALTIGDLVAFTTLQSRLFFPLGQLLNVQVEIQ
                     SALALFDRIFEYLDKPHEITDQPGALVLDPETVRGDVRYDQVAFKYGSGEAGEEGEDS
                     WSLQGIDFEARPGQLVALVGPSGAGKTTMTYLLPRLYDVDAGAIRIDGHDVRDVTLES
                     LGAVIGVVTQETHLFHSTIRENLRYGRPDATQEELEAAAKAAAIHDRIMELEDGYDTI
                     VGERGYRLSGGEKQRLAIARVVLKDPRILILDEATSALDTHSERLIQSALEPLMQGRT
                     TIAIAHRLSTILSADLILVIERGRIVERGTHGELLAEGGLYAHLYTEQFGGGEYESRC
                     EDGVIARDGHVIELAVAGDDD"
BASE COUNT         1118 a         2151 c         2042 g         1148 t
ORIGIN      
        1 ggagttcgcg tcgctcttcc tgcggatctg gtcgctgctg cgcgacgagg cggcagttgc
       61 cgcgcgggtg ccggcctgat ggccagtaat cgccgctggc tggcgctgct gctggcgttg
      121 tcgggcccga tcgtcgtcct cgcgatctgg gaggcgctct cgcgggccga ggcgatcaac
      181 ccgctcttct tcccggcccc gaccagcctc ttgccgacga cccgcgacct gatcgagtcc
      241 ggccagctct ggcgcgacac gcgcatcagc atgggccgcg tcctgaccgg tttcgccgtc
      301 gccgccgtcc cgggtgtcct gattgggatc atgatggcgc tctggtggcc actgcgggcg
      361 atgctgacgc cgatcgcggc agcgttcttc gcgattccga agatcgccgt cctgccgctc
      421 gtcatcatca tcttcggcat cggcgagacg tccaaggtcg caatggtcgc cgtcagtgtc
      481 ctcttcctgg tcgtgctatc gacgatgagc gccgtcctgg aggtcgagcg cgcctacttc
      541 gacgtcgccc gcaacgccgg cgccaactgg tggcagcagt tgcgaaccgt cgcactgccc
      601 ggcgcattac cgggcatctt ctccggcctg cgaatcgcgc tcggcttctc gctgctggtc
      661 atcgtcggga ccgagatgct ggccgccaag gacggcatcg gctacctgat ctggaactcg
      721 tatcaaacgt tcgccatcga gaagatgtac gtcgggctgg tcgtgacggc gctgctcggc
      781 tggctgctga acctggtcat ggacgagatc gaacgggtcg tcattccatg gcgagcgccc
      841 gaatcgcgcg gcatctcgct tccggttccg gagaagatgc gcaactggtt catggcgaca
      901 cggccgttct cgttcaccgc ctcggccgtc ccggtcctgc tcgggacgct actggccgcc
      961 cacgacggcg agttcgacct gctgctgttc gcgctcgtga tcgtcgcgtc agtcatcgtc
     1021 cacgccgcca gcaacctcgt caacgactac ttcgactgga agaaccgcgc cgacaagccg
     1081 gccgctctcg gacgcggcgg ctacatccag cgcggcatca tccggcctga agggatcctc
     1141 tggtttggtc tggcgctctt cggcgtcgcg acggcgatcg ggctggtcat cgtctacctg
     1201 gttggctggc cggtgctgct cttcgcgctg ccgagcctgg cagccgccta cttctacaca
     1261 ggcggaccga agccgctggg ctatgtcgcg ctcggcgagg cgaccgtctt cctcttcatg
     1321 gggccagtca tcgtcgtcgg cagctactat gtccagacgg gtgcgatcgc ctgggcgccg
     1381 gtcctcctct cggtcccggt cgggctactg gtgacggcca tcctgcaagc caacaacatc
     1441 cgcgacatcg ccgacgacac cgaggccggc aagcgaacgc tggccaccta catcggccgc
     1501 cgctgggcca accgcgagta tgtcctgctg ctgttcggct cgtaccttgt catcggcctg
     1561 atcgcgctcg gtggtgtcat tccggccggc gcgctgattg tcttcctgac cctcccgcgc
     1621 gccatcgaag tgacgcggat cgtcctccat cgcgacgaag cccgcttcct caatgccgcg
     1681 ttgcgccaga cggcaggtat ccacctccag tttgggacga tcctctccgc cgtcctgctt
     1741 gcgacggtcg tcttctagcc ctgcgactca ccggccagcc acgggccgta cccatcacgc
     1801 cgaagccaga acgagaagtc accgaaatcg aacgtgtcct tgtcggcgtc gcgctgatcc
     1861 ggcacccgtc cggaccagat ctcctcgtag gcgtagtgca ttgagcgcac ttcgtccggc
     1921 atcagcggaa cccggttgtg cgatggatac acgacatcca cgagatcggc gagttccgcc
     1981 agcagccgca gcgattcgcg ataggcgatc gggtcgctat acggtcgcca ggcgaacatc
     2041 ggaccggtgt agacggcatc acccggaaag agaagccggt tctcgcggtc gagcagcgta
     2101 atcccgcccg gtgagtgtcc tggagtgtga aacgcttcca acacccgccc gcccagatcg
     2161 aaacgctggc cgggctcaat cgcgccggtc ggctccgctg gctcgatccg tgcctgctcc
     2221 ggatcgaaat cgactggcag ccggtcgccg accagatgct gcggttcgaa ccatctgcga
     2281 aaacgctcat tcggaacacc ttcgcggagg tcgtcagcct cgctggcatg gacgaggaca
     2341 cgatcgaagc ggttgctggc gccgacgtgg tcaaaatggg cgtgtgaatg gaggacgatc
     2401 ggggcgcgat cgctcaacgt cgcgaccagc ccggcgaaat caccgacgcc catcccggtg
     2461 tcaaggatgg cgacatcccg ctcaccctcg acgagatagg acttcacatc ctccgagtgt
     2521 ccgggttcgg cgatcatcgt gacgccttgc ggaaaccgca ccacctcgaa ccagtcaagt
     2581 cgttccatct accgcctccg cccggtcagc acatatctca gccgcgagag actaccggcg
     2641 gctgaccgcc ccgtcaacag cgttgcagcg gcggctgttg acacgccgcc agccgcctcc
     2701 ctatcctgtt gctccggccc gcctggatcg gccggccgcc tggaatgagt ggagtgagcg
     2761 agacgatgac gacgccgacg ccgatgttcg acctgatcaa gaccctgaca gagataccgg
     2821 ggccaacagg gcaggaggat ctggtccacg agtggtgcgc ggaccactgg tcatcgttcg
     2881 ccgaagagac gttgatcacg cgcgtcggta acgtcgccgc ccgtgtcggt ggcgacggcc
     2941 cggcgatcgt cgtcctcgcg cacggcgatg aactcgggct gatgatcaag tcgatcaccg
     3001 acaacggatt gctgcacgtc tggccgatcg gccgtgaccc gcgcggtcgc ccgtcgcact
     3061 cgtacgcgcc cgtcaaccag ccggtcattg tcctcgctga cagtggtcag gtcgagggcc
     3121 aactcgtcta cgccagcggc cacgtcatcg gtggcggcaa ccagaaggac cacttcgagt
     3181 ggaacgactg gttcgtcgac ctcggctaca cgtcgcgcga gaaggtcgag tcgttcggta
     3241 tccaccccgg cacgcggatc gcactcaacc cgccgacccg gcggctgggc gacacgatcg
     3301 tcggcaaggc gatggacaac cgcgccgcgc tcgcgatcgc cacctcagtt ggagagcggg
     3361 ccgacacagc gaagttgaag taccagctct ggatcgccag ctcggttcag gaagaaaacg
     3421 gcctgctggg tgcatccagt attcccgaga tcgccgactt ctcgtacggg atcgcgcttg
     3481 atgtcgggct gtgcggcgac gtgccaggga ccagctccga gaaccatccg tcgaagctcg
     3541 gcaacgggcc gatcctcgtc catcaggact cgtcagtcca ctactcacac cggatgacga
     3601 tggcgctggc cgaaaccgcc gcgacggccg gaatcccggt ccagcacgcg atcttccaga
     3661 actacggctc ggacggggcc gagctgatcc ggcgcgggat cgaatcggtc ctgctgacga
     3721 tgccgacacg ctacacgcac tcgccgaacg agatgatcac cgaaaccgaa tgcgtccagt
     3781 gcgccgactt gatcctcgcc tggctggagc aggaaccgct gccgccgcgc tggacgaagg
     3841 cgtaacagcg gcacaatcgc tcagtgccgc cggctaccga tcagccgcga caggacaacg
     3901 attccggcga tgccgaagag gattccgaag gccagcggca acaggtcgcc gtccagacca
     3961 aaggtgatac ccgcgatgcc ggatgcacaa aggaccagcg cctggatcca ggcgaaccag
     4021 cggccggcac ctctgcgcgg tgggttggcg agtgtccgac cgacgatgta gccccacatg
     4081 tttcggctgt tgtctggatc atagggtgag cgcagatagc gggagaacat cgtctctgga
     4141 tagccgcctc gcaagcggcg tctgcgcttg tgtctgggcc ggtccggcac gtttcgccct
     4201 cgttccgatc gagcgttggg acctcatcgt acaccgaagg gcctcaacgc gtgatcggaa
     4261 gtaggcgatg cctgctcagt cgtcatcgcc ggcgacggcc agttcgatga cgtggccgtc
     4321 gcgggcgatg acgccgtctt cgcagcgcga ttcatactcg ccgccgccga actgctcggt
     4381 gtagaggtgg gcgtagagac caccctctgc cagcagttcc ccgtgcgtgc cgcgctccac
     4441 gatccggcca cgctcgatga cgaggatcag gtcggccgag agaatggtgg acagccggtg
     4501 ggcgatcgcg atcgtcgttc tgccctgcat cagcggttcc agcgccgatt ggatcagccg
     4561 ctcggagtgc gtgtcaagcg ccgacgttgc ctcgtcgagg atcagaatgc gcggatcctt
     4621 gaggacgaca cgggcaatcg ccagccgctg cttctcgccg ccggagagtc gatagccgcg
     4681 ctcgccgacg atcgtgtcgt agccgtcttc gagctccatg atccggtcgt ggatcgcggc
     4741 cgccttcgcg gcagcctcca gctcctcctg ggtcgcatcc ggccggccgt agcgcaggtt
     4801 ctcgcgaatc gtcgagtgga acaggtgcgt ctcctgcgtc accaccccga tgacggcgcc
     4861 gagcgattca agcgtcacat cgcgcacgtc gtgtccgtcg atgcggatcg cgccggcgtc
     4921 gacgtcgtag aggcgtggca gcaggtaggt catcgtcgtc ttgccggcgc cggacgggcc
     4981 gaccagcgcg acgagctgac ccgggcgagc ctcgaagtcg atgccctgca acgaccagct
     5041 atcctcgccc tcttcgcccg cctcaccgct tccgtacttg aacgctacct gatcgtagcg
     5101 aacatcgccc cgcacggtct ccgggtcgag aacaagcgct cccggctggt cggtgatctc
     5161 atgcggcttg tcgagatact cgaaaatgcg atcgaagagc gccagcgcgc tctggatctc
     5221 gacctggacg ttgagcagtt gaccgagcgg gaagaacagc cgcgactgaa gcgtcgtgaa
     5281 ggcgaccagg tcaccaatcg tcagtgccgc cgtctggccg gcgttgtcgg cctggatgat
     5341 cgagcgtccg gccagccagt aggctagcgc cggcgcgatc gagaagaagg tgccgacgat
     5401 catgaagaac cagcggccga tcatctgctg ccggatctgg agggcggcga gccgctcgtt
     5461 ctcgtcgtcg aagcggccga tctcgtacga ctggcggccg aaggtcttcg tcagcaggac
     5521 accgctgacc gagagcgtct cctcgaccat cgacgtcatg tcggccatcg agcgctgcgt
     5581 ctcgttcgcg atgatgcgcc gcttctggcc gaccttgtag gtcaggtaga gcgcaatcgg
     5641 catcagcgca aacgacagcg ccgtcagctc gaccgagatg acgaccatcg cgacgacggt
     5701 tgaaatcact gtcgtcaggt tcgagacgac actaccagcc gtgttggtga cgacgccctg
     5761 gacaccaccg acgtcgttag acaaacgcga ctgaatctca ccggtgcgcg tcgtcgtgaa
     5821 gaagcgcatt gacataccct gcaagtggcc atacagccgc atgcggatgt cgcgcatgac
     5881 gcgctggccg atcaggttgc tgaagtaggt ctggccaacc ccgatcagcc cgccgacgat
     5941 tggcgccagg atcatcaggc caacatagag ggtcagcagc gacatgtcgc cgcttgggat
     6001 ggcatcgtcg atgatcagct tgagcagaat cgggttggcg atgccgatga tcgaggtggc
     6061 gacgatcgcc agcagcacga agaagacatg cttccggtac ggccggaaga tgcgagcgac
     6121 gcggctcagt gtcctgccgt cctcggcgcg cgttcgcttt ggccggtcat ctggcgcggg
     6181 gccgccgata cgcccgccgc cacggcctcc catcgagtga tctctcaata gactgttgct
     6241 cctcatcgtg tgaagtcgta ttccgtcaag tcaattgtat gtcgaagcga accgcctgaa
     6301 acctccctgt tcgggcagga acgcatccgg cgttccggca ggttgactca tgcgtggtcg
     6361 tgcctcgaag ggagataatg cgtctcatgt cgtcctgagc cgcagcgaag gacctcccca
     6421 gccctgacgc cgcacgaagc tgacgcggag aggtccttc
//
LOCUS       DASUGJ010000754         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2069978, whole genome shotgun sequence.
ACCESSION   DASUGJ010000754 DASUGJ010000000
VERSION     DASUGJ010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069978"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFV93_17060"
     CDS             complement(<1..483)
                     /locus_tag="VFV93_17060"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEX5167118.1"
                     /translation="MIILVRNWWSLVLRGVLAVLFGVVAILWPDITLGALIILFGAFA
                     LVDGVFAIAGAINQAGDVPRWWVLLLQGLAGVVSGILAFVWPDLTALALLYIIAAWAV
                     VTGVLEIVAAIRLRQEIEGEWLMGLAGLMTFVFGLLLIVFPGSGAVALVWLIGAYAIA
                     T"
     gene            complement(480..3044)
                     /gene="lon"
                     /locus_tag="VFV93_17065"
     CDS             complement(480..3044)
                     /gene="lon"
                     /locus_tag="VFV93_17065"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5167119.1"
                     /translation="MVTEVQPTTPADVQIPDALPILPLGGSIVVFPFAVVPLVVAQPT
                     SVRLVDDAMSTNRLIALIAQRDEAHEPLGRDDLHSVGVVSIVHQLLRAPDGTLRLVVQ
                     GLERIRLEEIIQTEPYLIGRVEQAPDTYVPDVEVEGLRRALLDLFRRVVELTPNVSGD
                     IVAVVEQLADPRQIAYYVASTVPLSGAVRQEILEINPVDAKLRRLIEALQHEVTVREL
                     GERITSETQQRMNKAQREYFLREQLRSIQQELGEGEEGSELADLHRRVDEAGLPAEAR
                     READRELGRLASIPQASPEYGIIRTYLDWMLSLPWNTLTGGTIEIERARHVLNEDHYD
                     LEKIKDRILEYLAVRKLRQERQGRLHLASSDTSDEPQLPSVQTETLADGTAHEPILCF
                     VGPPGVGKTSLGQSIARAMGRKFVRMSLGGVRDEAEIRGHRRTYIGALPGRIIQGIRQ
                     AETRDPVFMLDEIDKVGTDWRGDPSSALLEVLDPAQNHSFVDNYLGVPFDLSQVLFIA
                     TANTLDSIPGPLQDRMEVLPLSGYTDEEKLQIARQYLVPKQVAAHGLEPGEIMLEDDA
                     IRRIIRGYTREAGVRNLGREIASICRKVAREIAEGRSESVTVDVSDVERYLGRARFQD
                     DVAERTDRPGVATGLAWTPAGGDVLFVEAAMIPSREERLILTGMLGEVMRESAQAAVT
                     WVRSNAERLGIDPALFEGMSIHLHVPAGAIPKDGPSAGVTMMTALVSLASGRPVHSDV
                     AMTGEITLRGKVLPVGGIKEKVLAANRAGIETVILPRQNERDLDDVPDELRRELEFVL
                     VESAEEVIERALPLEALSGRGAPRVMGEAYGREDPLAASARSDNRENPHQRGGWSA"
BASE COUNT          573 a         1073 c          984 g          571 t
ORIGIN      
        1 agtggcgatg gcgtaagcgc cgatgagcca gacgagcgcc acggcgccac tgccgggaaa
       61 cacaatcagc agcagcccga agacgaatgt catcaggccg gccagcccca tcagccactc
      121 gccttcaatt tcctgccgca accgaattgc agccacgatc tccagtacgc cggtgaccac
      181 cgcccaggcg gcgatgatgt agagcagcgc gagtgcggtc aaatccggcc agacgaaggc
      241 caggatgccg gagacgactc ccgccagtcc ttgcaggagg agcacccacc agcgtggaac
      301 gtcaccggct tggttaatgg cgccggcgat cgcaaagact ccgtctacca gcgcgaaagc
      361 gccgaagagg atgatcagcg cgcccagggt gatgtccggc cagagaatgg caacgacgcc
      421 gaagagcacg gcgagcacac cacgtaacac aagcgaccac cagtttcgga ccaggattat
      481 catgcgctcc aacctccacg ttgatgagga ttctctcgat tgtcagaccg tgcgcttgcg
      541 gcgagtggat cttcgcggcc gtatgcttct cccatcactc tgggcgcccc acgaccggat
      601 aacgcttcta gcggtaacgc gcgctcgatc acctcctcgg cgctctcgac cagcacgaac
      661 tccagttccc ggcgcagttc atccggcacg tcatcgaggt cgcgctcgtt ctgacgcggc
      721 aggatgaccg tctcgatacc ggcccggtta gcagcgagta ccttctcctt gatgccgcca
      781 accggcagaa ccttgccgcg cagtgtgatc tcgccggtca tggccacgtc gctgtgcact
      841 ggtcggccgc tggcgagcga caccagcgcc gtcatcatgg tcaccccggc ggaagggccg
      901 tccttcggga tggcgccggc cggaacgtgc aggtggatgc tcatgccctc gaacagcgcc
      961 gggtcgattc ctaatcgctc ggcgttcgag cgcacccacg tcaccgccgc ctgggcgctc
     1021 tcgcgcatca cttcgcccag catgccggtc aggatcagcc gttcttcgcg gctcgggatc
     1081 atcgccgcct cgacgaagag cacatcgccg ccggccggcg tccaggccag gccagtcgcg
     1141 acaccggggc gatcggtgcg ctcggccacg tcgtcctgga atcgcgcccg ccccagatag
     1201 cgctccacgt cactcacgtc gacagttacc gattcggatc gaccttcagc tatctcgcgc
     1261 gccaccttgc ggcagatgct ggcgatctca cgtccgaggt tgcgcacacc cgcctcgcgt
     1321 gtgtagccgc ggatgatgcg ccggatcgcg tcgtcttcga gcatgatctc gccgggttcg
     1381 agcccatgag cggcaacctg ctttgggacg aggtactggc gagcaatctg gagcttctct
     1441 tcgtcggtgt agcccgagag cggcaacacc tccatgcggt cctgcaatgg acctggaatg
     1501 gaatcgagcg tgttcgccgt cgcgatgaaa agtacttgcg agaggtcgaa cggcacaccg
     1561 agatagttgt ccacgaagct atggttctgg gccgggtcga ggacctcgag tagtgccgac
     1621 gacgggtcac cacgccagtc ggtgccgacc ttatcgatct catccagcat gaagaccggg
     1681 tcgcgcgtct ccgcctgccg gataccctgg atgatccggc ccggcagcgc gccaatgtag
     1741 gtacgccggt gcccgcggat ctcggcctcg tcgcgcacgc cgcccagcga catgcgtacg
     1801 aacttgcgcc ccatcgcgcg cgcgatcgac tgaccaagac ttgtcttgcc gacgcccggg
     1861 ggaccgacga agcataggat cggctcgtgc gccgtgccgt cggcaagcgt ctcggtctgc
     1921 acgctcggca gctgcggctc gtcgctcgtg tcgctgctcg ccaggtggag tcgcccctgt
     1981 cgctcctggc gcagtttccg cacggccagg tactcaagga tgcggtcctt gatcttctcc
     2041 aggtcgtagt ggtcctcgtt cagcacgtgc cgcgcccgct cgatctcgat cgtgccgccg
     2101 gtgagcgtgt tccacggcag gctcaacatc caatcaaggt aggtgcggat aattccgtac
     2161 tcaggcgacg cctgcggaat actcgccagc cggcccagtt cccgatccgc ctcgcggcgc
     2221 gcctcggccg gcagtccggc ctcgtcgacg cgacggtgta gatcggccag ctcggagcct
     2281 tcttcgccct cgcccagctc ctgctggatc gagcgcagtt gctcgcgcag gaaatactcg
     2341 cgttgtgcct tgttcattcg ttgctgcgtc tcggatgtga tccgttcccc caattcgcga
     2401 acggtcacct catgctgcag cgcttcgatg agacgacgca gcttggcatc cactggattg
     2461 atctcgagaa tctcctggcg caccgcgccg gagagaggca cggtcgacgc aacgtagtag
     2521 gcgatctgtc gaggatcggc gagttgttcg acgaccgcga cgatgtcgcc tgagacgttg
     2581 ggagtgagct caaccacgcg acggaagaga tcgagcaacg cccgccgtag cccttcgacc
     2641 tcgacatcgg gcacgtatgt gtccggcgcc tgctcgaccc gtccgatcag gtacggctca
     2701 gtctggatga tctcctcaag ccggatgcgc tctaagccct gaacgaccag ccgcaatgtg
     2761 ccgtcgggcg cacgcaagag ctgatggacg atgctcacaa cacccaccga gtgcaggtcg
     2821 tcccgcccaa gtggttcgtg agcctcgtcg cgctgggcga tcaaggcgat cagccggttc
     2881 gtgctcatcg cgtcatctac cagccggacc gacgtcggct gagcaacgac aagcggcacg
     2941 accgcgaatg gaaacacgac gatgctccca cctagcggaa gaatcggcag ggcatccggg
     3001 atctggacat cggccggtgt cgtcggttgg acttcagtca ccatattgtt tacctcacgt
     3061 cggtctttgg aaacgtgatc tgcagcaggc cgcgatcgta ggtcgcgtcg atccgctcca
     3121 gatcacgcgt cgccggtagt ggcagctcca gccggaaggc cccttgtcgg atctcgacgc
     3181 gatggtagag gccgggtgtt g
//
LOCUS       DASUGJ010000755         3192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1375252, whole genome shotgun sequence.
ACCESSION   DASUGJ010000755 DASUGJ010000000
VERSION     DASUGJ010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375252"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..604)
                     /locus_tag="VFV93_17070"
     CDS             complement(47..604)
                     /locus_tag="VFV93_17070"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEX5167120.1"
                     /translation="MSLEQRTVVWRGIAYPSMEFFNVHQHAGGWLLTGSVVAVIDDLP
                     LQVNYEVGCDAGWQTSHVDLTLVTAAGERRRRLEAPGDGRWLQTGQPIAEVAGCIDID
                     LGITPATNLLPIRRLNLAVGQSADVVATWVRFPTLASQPLSQRYTRLTEDSYRYESDT
                     GFSADLTVDADGLVTTYPGGWERVV"
     gene            complement(604..1146)
                     /locus_tag="VFV93_17075"
     CDS             complement(604..1146)
                     /locus_tag="VFV93_17075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5167121.1"
                     /translation="MEHQDHVALLRDGVDGGGVWADLGSGGGAFTLALADLLGPGSTI
                     YSVDRDGRGLRRQREAMQASFPDVAVHYIEADYTGRLDLPPLDGVVMANALHFQRKRR
                     KGEVLDLIHGYLKPGGRLVIVEYNTDRGNLWVPHAFSYDTWTQLASAHGFSDTRQLAT
                     RPSRHLREIYSAITFKGGEQ"
     gene            1469..>3192
                     /locus_tag="VFV93_17080"
     CDS             1469..>3192
                     /locus_tag="VFV93_17080"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5167122.1"
                     /translation="MIAIFVAAWRVMLKRVRASGLVLTTAFTTILLAITLLAAGPIYA
                     NSVYLAALQRTIRDAPIREANLEVSATLQPAALAPADEIVSTEIERTFATGSVAIYRT
                     GVSESYALPHADDTDVTDLTVFRYYEDLEDHTALIGGEWPATGTSTDTVEVAISEGTA
                     LELQLAIGDDVPVVNRLDETKTVTAIVSGIFAVDDSLDPYWYANELEVAGVEPGSSFT
                     RYGPFVVDEAAFMRVFNQRPATVSWRVVPLIDKMTVDDLPRLRSALSNLEQRIDSGAQ
                     IDSRITGSIELAIMLGKVERSLLATRSGVLILTVQLAILAGYALVLSAGLLVDQRRSE
                     TVLLRSRGAGVRQMLALALMEGAVLTVPAVVVGPFLAAWLLRLLNVIGPLAGIGLTLH
                     PQVTRTSFVLGGLAGLAAIVTLALPAIASARSSIEGQGGRSRARTRGIWQRGAIDITL
                     LAVAGLAFYQLRRYGLPLTESVRGRLEVDPLLVAAPAIGLLAGALIALRLVPLLAAFA
                     ERLTTRRGRAVTVLSAWQVARRPLRYARSALLLILALAIGLFTVSYSSTWRVSQGDQA
                     DFQIGADVR"
BASE COUNT          494 a         1063 c         1083 g          552 t
ORIGIN      
        1 gttcctcacc gatgccgttc ggccgacagg cgcctggata gaggcatcag acgacacgct
       61 cccagccgcc ggggtaggtg gtgacgaggc cgtcggcgtc gacggtcagg tcggcgctga
      121 agccggtgtc gctctcgtag cggtagctgt cctcggtcag acgggtgtag cgctggctga
      181 ggggctggct agccagcgtt ggaaagcgga cccaggtcgc cacgacatcg gcgctctgac
      241 ccactgccag attcaaccga cggatcggga gcaggttggt ggccggagtg atgccgaggt
      301 cgatatcgat gcagcccgcg acctcggcaa tcggttggcc agtctgtagc catcgtccgt
      361 cgccgggcgc ttccagccgg cggcgacgtt caccggcggc ggtgacgagc gtcagatcga
      421 cgtggctggt ctgccagccg gcgtcgcagc cgacctcgta gttgacctgc aacggaagat
      481 cgtcgatgac agcgacgacg ctaccggtca acagccagcc gccggcgtgc tggtggacgt
      541 tgaagaattc catcgacggg tacgcgatcc cacgccagac gacggtccgt tgctccaggc
      601 tcatcactgc tcgcctccct tgaaggtgat ggccgaatag atctcgcgca ggtggcggct
      661 gggccgggtc gccagttggc gcgtgtcact gaaaccgtgg gcggaagcga gctgtgtcca
      721 ggtatcgtag gagaacgcgt gcggcaccca gagattgcca cggtcggtgt tgtactcgac
      781 aatcaccagc cgcccacccg gtttcaggta gccgtggatc aggtcgagta cctcaccctt
      841 ccggcgcttg cgctggaagt ggagcgcgtt ggccatcacg acgccgtcga gcggcggcag
      901 gtccagccgg ccggtgtagt cggcctcgat gtagtggacc gcaacatccg ggaagctcgc
      961 ctgcatcgcc tcgcgctggc gacggagccc gcggccgtcg cggtcgaccg agtagatcgt
     1021 actgcccggc cccagcagat cggccagcgc cagcgtaaac gcgccaccgc cggaccccag
     1081 atcagcccag actccaccac cgtcgacgcc atcccgcagc agcgccacat gatcctgatg
     1141 ttccaaccag cctcccgtcg ctgtcgaccc gggctgcatg gtacttgacg tgggcgggta
     1201 tcgggaatcg ggattcgggg ttgggttacg gcacacggcc cctggtcatg cagggtggtg
     1261 gtggaagatc acgacgtaac ccaaccccga atcccgattc ccgatacccg cccaccacac
     1321 acccaaaagc cctctccgga cgtacactgg agtacacgct gatccgtgtg ggggatccac
     1381 gggtaatcgc tgacaccagt ctcaccggga gccgtatgct gtctgcctgg cgccggaatg
     1441 cgcgtgggtc gagcgagcag atacgccgat gatcgcgatc ttcgtcgcgg cctggcgggt
     1501 gatgctcaag cgagtgcgcg cctccgggct ggtgctgaca accgccttca cgacgattct
     1561 gctggcgatc acgctgctgg cggccgggcc gatctacgcc aactcggtct acctggcggc
     1621 gttgcagcgc acgatccgcg acgcgccgat tcgcgaagcc aacctggaag tctcggcgac
     1681 cctacagccg gccgccctcg cgccggccga cgagatcgtc tcgaccgaga tcgagcggac
     1741 gttcgcgacc ggcagcgtcg cgatctaccg aaccggcgtg tccgagagct acgccctgcc
     1801 acatgctgat gacacggacg tcaccgacct gaccgtcttc cgctactacg aggaccttga
     1861 ggaccacacg gcgctgatcg ggggcgagtg gccggcgact gggaccagca ccgacaccgt
     1921 tgaggtcgcc atctccgaag gcacggcgct cgagctccag ctcgcgatcg gcgacgatgt
     1981 gccggtcgtc aaccggctgg acgagacgaa gaccgtgacc gccatcgtca gcggcatctt
     2041 tgccgtcgac gactcgcttg acccgtactg gtacgccaac gagcttgagg ttgccggcgt
     2101 cgagccgggc tcgtcgttca cccgctatgg tccgttcgtc gtcgacgaag ctgccttcat
     2161 gcgcgtgttc aaccagcgac cggcgacggt gtcgtggcgg gtcgtcccat tgatcgacaa
     2221 gatgacggtt gacgacctgc cgcgcctgcg ctccgcgctc tcgaacctcg agcagcgaat
     2281 cgacagtggc gcgcagatcg actcacgcat caccggcagc atcgagcttg cgatcatgct
     2341 cggtaaggtc gagcgttcgc tgctggcaac ccgttccggc gtcctgattc tgaccgtcca
     2401 gttggcgatc ctggccggct acgcgttggt gctgtcggcc gggctgctgg tcgatcagcg
     2461 ccgcagcgag acagtccttt tgcgctcgcg gggggccggt gtgcggcaga tgctggcgct
     2521 ggcgctgatg gagggtgccg tcctgacggt gccggccgtc gtcgtcggtc cgttcctggc
     2581 ggcctggctg ctgcggttgc tcaacgtcat cggaccgttg gcggggatcg ggctgacgct
     2641 gcacccacag gtcacgcgaa cgtcgttcgt gctcgggggt ctggccgggc tggccgcgat
     2701 cgtgacgctg gcgctgccgg ccatcgcctc ggcccgatcg agcatcgagg gacagggcgg
     2761 gcgcagccgg gcgcggacac ggggtatctg gcagcggggc gcaatcgata tcacgctgct
     2821 ggcagtcgca gggctggcct tctaccagct ccgccgctac ggcctgccgc tgaccgagtc
     2881 ggttcgcggg cgcctggagg tcgatccgct actggtcgcc gcgccggcca tcggcctgct
     2941 ggccggcgca ctgatcgcgt tgcgactcgt cccgctgctg gcggcgttcg ccgagcgatt
     3001 gacgacccgg cgggggcggg ccgtcaccgt cctgagtgcc tggcaggttg cacgacgccc
     3061 gctgcgctat gcgcgctcgg cgctgctctt gatcctggcg ctggcaatcg ggctcttcac
     3121 cgtctcctac tcgtcaacct ggcgcgtctc gcagggcgat caggccgatt tccagatcgg
     3181 tgccgatgtc cg
//
LOCUS       DASUGJ010000756         5268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_264993, whole genome shotgun sequence.
ACCESSION   DASUGJ010000756 DASUGJ010000000
VERSION     DASUGJ010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264993"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFV93_17085"
     CDS             complement(<1..199)
                     /locus_tag="VFV93_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167123.1"
                     /translation="MAERDEWSRNDPDDLAPFVHPAEADFASFLDFYRIRWQYEPRSF
                     PLRWRDGRIAEMFTPDFYLPER"
     gene            complement(256..1350)
                     /locus_tag="VFV93_17090"
     CDS             complement(256..1350)
                     /locus_tag="VFV93_17090"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5167124.1"
                     /translation="MGQATDPDNLPDVGEDEQLDDSRLPPGERRARARRLVEFARLRR
                     SLLIRFAIAIAVVTGIYLVNSAFLGWGTFVTAAILAVPTSRFRAYTAAFLPYGLVWLV
                     FSFLRSLADETSVPLRTVQVTAVERALFFGTTPTIWLQEHLFVPGVIHWYDYATTFVH
                     WSYFFVPHIAAILIWRKSPALYNRYLLATMITLFVGLLVYFLSPAAPPWLTADAAPQT
                     DIFRVMVSVGRNINSSLFDRTYGVLGDPNPVAAMPSLHEAITFMLFWFARHHSRWLAS
                     LAFLYALAMAFALVYTGEHYVLDVLVGAAIATYAYFFAGRWLNVTAPIFGGQTALLIG
                     SSGGHDGLPPPRSAVREPALRDDRKREEPA"
     gene            complement(1363..1893)
                     /locus_tag="VFV93_17095"
     CDS             complement(1363..1893)
                     /locus_tag="VFV93_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX5167125.1"
                     /translation="MYWNRKHVLVCTASHCMQKGANNVAGRLRLEMKRSGLDKDVLVN
                     TCDSIDLCDIGPNIVVYPEQVIYSHVQVSDLKEIMEHLRGGEPVQRLMLTPETPEEAQ
                     RESFYRSVVANSDTVPSERFIEQAAEVGYDEAWINEQARRGFIARKDADGIPTITVTT
                     KALQRYRITVPTNERA"
     gene            complement(1944..2258)
                     /locus_tag="VFV93_17100"
     CDS             complement(1944..2258)
                     /locus_tag="VFV93_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX5167126.1"
                     /translation="MSAVYDGIKDRVGLERKLSINSYSVDPTRAHIRIIDHMVCLQCE
                     RQQCINCCPAACYAPQPDGTVLFSYEGCVECGTCRIVCHEFDNIEWTYPRGGFGIQYR
                     FG"
     gene            complement(2342..3184)
                     /locus_tag="VFV93_17105"
     CDS             complement(2342..3184)
                     /locus_tag="VFV93_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX5167127.1"
                     /translation="MGRWKDGAQCAVMLTFDVDAETLWLSGDMANMNRPGMLSQGTYG
                     ARVAVPLILDLLGERDLTGTFFVPGWTAENHRDIITVVHKMGHELGHHGWIHEWPTNL
                     NRQEEEEVLNKGIAALSAITGESPRGYRSPAWEFSANTLDLLAEHGFSYSSNLMSHFI
                     PWHHEAHDIVELPVQWLLDDAPFFLYGGGRPRPISSAENVYQAWTEEFRGIYRYGGLF
                     NLTMHPQIIGRPGRLMMLERMIDFINGFPDIWFATGSEIADYWRAHSRIHHPQTQNPP
                     EPAG"
     gene            complement(3220..3906)
                     /locus_tag="VFV93_17110"
     CDS             complement(3220..3906)
                     /locus_tag="VFV93_17110"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX5167128.1"
                     /translation="MSEQGTTTTYANFWIYRVDPAWRRQDSAEKSAARAEFATVFEKA
                     AAEGVSLRGAYSTVGLRADADLIFWVVTNDLDAMQRLAIAINGSALGAWLEPRYTFLG
                     VSLGSRYTADHAPAFVKGVPPKRYLSVYPFVKTHEWYQVPFEERRAAMAEHGQMGREY
                     PGILTNTISSFGIADHEFVVAFESDDVGEMVKMVEYLRPAASRPYTKLDTPIVLGVMK
                     DLPDVLADLG"
     gene            complement(3982..>5268)
                     /locus_tag="VFV93_17115"
     CDS             complement(3982..>5268)
                     /locus_tag="VFV93_17115"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5167129.1"
                     /translation="DEDDVALDTSMPALAETRLANGLRVMTLEATDRDPNGPVVVRLR
                     IPGGGALDGDRPGVARFTAEMLTRGSGGRTLDDLAEELDGLGASISAGAGRENTDLSL
                     TSMREDVERVMQLLATALLSPDFPAEQLEIVRAQLLSGLRQAEQDTRATAELELRRML
                     YPEGHPYRERVSGDAESVTAMTREDLLAFAERAYKPSDAIVAVAGGLSHGEAVDLVQR
                     HLGAWEGQAPSLVIPDVPVADATERVTRALPGKSQADIAIGGITIPRLNPDFYALNVA
                     NLILGRLGLMGRLGESVRERQGLAYYAFSGLEVGLGTGFWSARAGVNPENVERAIETI
                     LEEVRAFLKDGPTSDEFSDAIGNLTGSLPLGLETVGSIASIIADIGFFDLGLDYLQRY
                     RATIRALTPEQLVETMRRYVDPDRLAIAVVEPERPS"
BASE COUNT          969 a         1715 c         1690 g          894 t
ORIGIN      
        1 cgcgctcggg caggtagaag tcgggcgtga acatctcggc gatccggccg tcccgccagc
       61 gcaacggaaa tgagcgcggc tcgtactgcc agcgaatgcg gtagaaatcc aggaacgagg
      121 cgaagtcagc ctcggccgga tggacgaacg gcgcgaggtc gtccgggtcg ttgcgggacc
      181 actcgtcccg ttcagccatc acatcatccg tttggagagc gtcctcccct ggcggcggcg
      241 actcggctgc ctcggtcacg ccggctcctc tcgcttcctg tcgtcgcgta aggccggctc
      301 gcgcacggcc gaacgcggtg gcggcaggcc atcatgcccg ccggacgaac caatcagcag
      361 cgccgtctgc ccgccaaaga tcggcgcggt gacgttcagc cagcgaccgg cgaagaagta
      421 ggcgtaggtc gcgatcgcag cgccgaccag cacatccaga acgtagtgct cgccggtgta
      481 gacgagtgcg aacgccatcg ccagtgcata gaggaacgcc agcgacgcca gccagcggct
      541 gtgatgccgc gcgaaccaga acagcatgaa cgtgatcgcc tcgtgcagcg acggcatcgc
      601 cgcgacggga ttgggatcgc cgagcacgcc gtaggtacgg tcgaacaggg acgagttgat
      661 gttgcgaccg acgctgacca tcacccggaa gatgtcggtc tgtggcgcgg catcggctgt
      721 cagccacggc ggcgcggccg gcgagaggaa gtagactagc agtccgacga acagcgtgat
      781 catcgtcgcc agcaggtagc ggttgtagag ggccgggctc ttacgccaga tcagaatcgc
      841 ggcgatgtgc gggacgaaga agtacgacca gtggacgaag gttgtcgcgt aatcgtacca
      901 gtggatgacg cccggcacga acaagtgctc ctggagccag atcgtcggag tcgtgccgaa
      961 aaacagcgcc cgctcgacag cagtgacctg gaccgtccgg agcgggactg acgtctcgtc
     1021 agccagcgaa cgcaggaacg agaagacgag ccagacgagg ccgtacggca ggaacgcggc
     1081 tgtgtacgcg cgaaagcgcg aggttgggac ggcgagaatc gccgccgtca cgaatgttcc
     1141 ccagccgagg aacgcagagt ttacgaggta gatgccggtg acaactgcga tcgcgatcgc
     1201 gaagcggatg agcagcgatc gtcggagccg ggcgaactcc accaggcgcc gggcacgcgc
     1261 gcggcgttcg ccgggcggca gtctggaatc gtccagctgc tcgtcttcgc ctacgtcagg
     1321 caggttatcg ggatctgtcg cttggcccac gatgtcaatc aatcacgcac gttcgttggt
     1381 cgggaccgtg atgcgatagc gctgcagcgc cttggtcgtt actgtgatag ttgggatacc
     1441 gtccgcgtcc ttgcgggcga tgaagcctcg ccgtgcctgc tcattgatcc aggcctcgtc
     1501 gtagcccacc tcggccgcct gttcaatgaa ccgttcagat ggcaccgtgt cactgttcgc
     1561 aacgaccgat cgatagaacg actcgcgctg ggcctcttcg ggcgtctcgg gcgtcagcat
     1621 cagccgctga accggctcac cgccgcgcag gtgctccatg atctccttca gatcggagac
     1681 ctgaacgtgg ctgtagatga cctgctcggg atagacgacg atgttcggac cgatgtcgca
     1741 gagatcgatc gaatcgcagg tattaaccag cacatccttg tcgagtccgc tgcgcttcat
     1801 ctccagccgc aggcgtcctg caacgttgtt ggcgcccttc tgcatgcagt gcgatgccgt
     1861 gcagacgagc acgtgcttcc gattccagta catcctcttc acccctcatc cagcgtcgcg
     1921 gcctggcggg ttgccgcgcc aggctacccg aagcggtact ggatgccgaa gccgccacgc
     1981 ggataggtcc actcgatatt atcgaactcg tgacagacga tccggcacgt cccgcattcg
     2041 acgcagccct cgtacgagaa cagcaccgtc ccatccggct gtggcgcgta gcaggccgcc
     2101 gggcagcagt tgatgcactg ctgtcgctcg cattgcaggc agaccatgtg gtcgatgatg
     2161 cgaatatggg cgcgcgtcgg atcgactgaa tacgaattga tgctgagctt gcgttcgagc
     2221 ccaactcgat ccttgattcc gtcgtaaacg gcactcacca gttacaccag accctctgtt
     2281 gcagcccctg tcagtcaacc tagtctatca tcgcggaacg ccggttccgg acgacgagac
     2341 gtcaaccggc aggctcggga gggttctggg tctgcggatg atggatgcgg ctgtgcgcgc
     2401 gccagtagtc ggcaatctcg gagccagtcg cgaaccagat atccgggaag ccgttgatga
     2461 agtcgatcat tcgctccagc atcatcaacc ggcccggccg gccgataatc tgcgggtgca
     2521 tcgtcaggtt gaacagcccg ccgtagcggt agatgccacg gaactcctcc gtccaggcct
     2581 ggtagacgtt ctcggcgctg ctgatcgggc gtggccggcc gccaccgtag aggaagaacg
     2641 gagcgtcgtc caacagccac tggaccggca actcgacaat gtcgtgcgcc tcgtgatgcc
     2701 aggggatgaa gtggctcatc agattagagg aataggagaa accgtgctcc gccaacagat
     2761 cgagcgtgtt ggccgagaac tcccaggccg gcgagcggta gcctcgcggt gattcgccgg
     2821 tgatggccga gagggcggcg attcccttgt tcaggacctc ttcctcttcc tgacggttca
     2881 ggttagtcgg ccactcgtga atccaaccgt gatgccccag ttcgtggccc atcttgtgga
     2941 cgacggtaat gatgtcgcgg tggttctcgg ccgtccagcc cggcacgaag aaggtccctg
     3001 tcaggtcgcg ctccccgagc agatcgagga tcagcggaac ggcaacccgc gcgccgtagg
     3061 tgccctgcga caacatgccc ggccggttca tgttcgccat atcgccggag agccagagcg
     3121 tctccgcgtc gacatcgaac gtgagcatga cggcgcactg cgcgccatcc ttccagcgtc
     3181 ccactctggc cctcccgact ctccgacggc ctgcgacgat cagccaagat cggcgaggac
     3241 gtccggcaga tccttcatca cgcccagcac aatcggcgtg tccagcttgg tatacgggcg
     3301 cgacgcggcc ggtcgcaggt actcgaccat cttgaccatc tcacccacgt cgtcggactc
     3361 gaaagccacg acgaactcgt ggtcggcgat gccgaacgac gagatcgtgt tggtcaggat
     3421 gccgggatac tcgcggccca tctggccatg ctcggccatc gccgcccggc gctcctcgaa
     3481 cggcacctga taccactcgt gcgtcttgac gaacgggtag acggagagat agcgcttcgg
     3541 cggaacgccc ttgacgaacg ccggcgcatg atcggcggta taacgagagc caagcgagac
     3601 acccagaaag gtgtagcgcg gttccagcca ggcgccgagc gcggagccgt tgatggcgat
     3661 cgccagccgc tgcatggcgt cgagatcgtt ggtgacgacc cagaagatca ggtcggcgtc
     3721 ggcgcgcagg ccaaccgtcg agtacgcgcc gcgcagcgag acaccttcgg cggcagcctt
     3781 ctcgaagacg gtcgcgaact ccgccctggc ggcggacttc tcggcgctgt cctgccggcg
     3841 ccaggccgga tcgacgcggt aaatccagaa gttggcgtag gtcgtggtcg tgccctgttc
     3901 gctcatcacc cgtgtccctg tatccgttcg cgttggcatc ccgacgagcc ggtcgatacc
     3961 ggtctgatta tgccaccagc gttacgacgg tcgttccggc tcgacgacgg cgatcgccag
     4021 ccggtcggga tcgacatagc gtcgcatcgt ctcgaccagt tgctcgggcg tcagcgcccg
     4081 aatcgtcgct cggtaacgct gcaggtagtc caggccgagg tcgaagaagc cgatgtcggc
     4141 gatgatcgag gcgatgctgc caaccgtttc gaggccgagc ggcaacgagc cggtcaggtt
     4201 cccgatcgcg tcgctgaact cgtctgaggt tggcccgtcc ttcaggaagg cacggacttc
     4261 ttcgaggatc gtctcgatcg ctcgctcaac gttctccgga ttgacgccgg cccgcgcgct
     4321 ccagaagccc gtcccaagtc cgacctccag gccggagaag gcgtaatacg ccaatccctg
     4381 ccgctcgcga acgctctcgc cgagccggcc catcagcccg agccgaccga gaatcaggtt
     4441 ggcgacgttg agcgcgtaga agtcgggatt caggcgcggg atcgtgatcc caccgatcgc
     4501 gatatcggcc tgcgacttgc ccggcaacgc gcgtgtcacc cgttcggtcg cgtctgccac
     4561 gggaacatcg ggaatgacca gcgacggcgc ttgcccttcc caggcgccga ggtggcgctg
     4621 aacgagatcg acggcttccc cgtggctcaa cccaccagcg acggcgacga tcgcatccga
     4681 cggcttatac gcgcgctcgg cgaacgccag cagatcctcg cgggtcatcg ctgtgacaga
     4741 ctcggcatcg ccgctgacgc gctcgcgata cggatgtcct tccggataga gcatccgccg
     4801 cagttccaat tcggccgtcg cccgcgtgtc ctgctccgcc tggcgcaggc ccgagagcag
     4861 ttgcgcgcga acgatctcca gttgctcggc cgggaagtcg ggcgacaata gcgccgtcgc
     4921 cagcaactgc atgacccgct cgacatcctc gcgcatcgac gtcaacgata ggtcggtgtt
     4981 ctcacggccg gcgccggccg agatcgatgc accaagccca tccagctctt cggccaggtc
     5041 gtccagcgtc cgcccgccgc tgccgcgcgt cagcatctcg gccgtgaagc gtgcgacgcc
     5101 aggccggtcg ccgtcgagcg cgccgccacc agggatgcgc aggcggacga cgaccggacc
     5161 gttcgggtcg cgatcggttg cctccagcgt catcacccgc aggccgttcg cgagccgcgt
     5221 ctcggccagt gccggcatcg acgtgtcgag cgcgacatcg tcctcgtc
//
LOCUS       DASUGJ010000757         4263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_123436, whole genome shotgun sequence.
ACCESSION   DASUGJ010000757 DASUGJ010000000
VERSION     DASUGJ010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123436"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..2528
                     /locus_tag="VFV93_17120"
     CDS             228..2528
                     /locus_tag="VFV93_17120"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5167130.1"
                     /translation="MSLAAFLDRLNREERWASCIAAWKTVPAQEAITAPIPGALDPRL
                     RRALIARGVDELYVHQADAFDAVITGNNTVVVTPTASGKTLCYNLPVLNHLLSEPTAR
                     AMYLFPTKALAQDQYAGLQALLDAAGADIKTWPYDGDTPAAERRLIRQAGHIIITNPD
                     MLHSAILPHHTKWHALFENLKYVVIDEMHGYRGVFGSHVANVIRRLKRICRHYGSSPR
                     FILASATIANPGELAEKLIELPVEVITRNGAPRGRKDFILYNPPVVNRALGIRRSAIL
                     EASGLASELIRNDIHTIVFARARVTAEVLLTYLREALPPKIGQSNAIRGYRGGYLPNQ
                     RREIEAGLRDGRIKGVVSTNALELGVDIGSLDAAVMTGYPGTIASAWQQAGRAGRRES
                     MSAAIMVASSSPIDQFIVNHPEYFFEGSPENGLINPDNLLIVVNHIKCAAFELPFKRG
                     EMFGTYEPTDALDYLAEIDILHYVNNTWHWMSDSYPAEDLSLRTAARENVVIIEKGDG
                     RPRVIGEVDAFSAPMLVHTDAIYIHDGQQYHVDELDWEEKKAYVKPVDVEHYTDASLS
                     VRVEVIEQFSDGDPAAERHHGEVLVGAIVTQFKKIRMHTHENLGWGKVFLPESQMHTS
                     AYWTSLPAYLSERMAEPVLHGALSGLANLLGNIAPVYLMCDPRDLGVYAQVRAPFTGA
                     ATVFIYDKVPGGIGFSEKLYEAHHLLLKAALDHVTRCPCQDGCPSCVGVRPSDESLPF
                     AKKTTRQLLEVLHMRTPRSYGQHYPE"
     gene            2584..3060
                     /locus_tag="VFV93_17125"
     CDS             2584..3060
                     /locus_tag="VFV93_17125"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5167131.1"
                     /translation="MTVEQVLPLLAATPQRIVAATDGLAPMQLRTAPEHDDWSANDVL
                     SHLRSCADVWGDCIATILAEERPTIRAIDPRTWMKQTNYPLLEFQPSLQEFLAQRADL
                     LAVLERLTPDDWSRGATVTGAGAPLERTVLSYAQRLARHERSHIKQIQRIVSTFQT"
     gene            3165..3836
                     /locus_tag="VFV93_17130"
     CDS             3165..3836
                     /locus_tag="VFV93_17130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5167132.1"
                     /translation="MGNWVAIGPAGTRGYLAVPESGSGPGLLVLHAWWGLTSAFTDIC
                     DQLAAEGYVALAPSLYASGATAETIAEAEALMSSHDEGTESKAIALAATDQLLSLPAV
                     SGSKIGVIGYSMGAYWALQLSQLRPENVSAAVTVYGTSDGDFGAAKAAYLGHFAEHDD
                     YEPLEGVRALEEQIRDAGCEVAFFIYPGTSHWFVEPNQPNAYNAEAAQLAWDRTLAFL
                     RAKLA"
     gene            complement(4049..>4263)
                     /locus_tag="VFV93_17135"
     CDS             complement(4049..>4263)
                     /locus_tag="VFV93_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167133.1"
                     /translation="FDAERTFAINWEDGAVRDVPAGVVLMQALHHGTEHRAHICTILT
                     SLGIAIPDLGLWDWAEATNRATLRSA"
BASE COUNT          781 a         1442 c         1331 g          709 t
ORIGIN      
        1 aacgcacgcg actgacgcgg agaggtcctt cgctgcggct caggacgaca gggggagctt
       61 cctcgctccg tttcgtccag attctcgacg cgggagaata gcaccggaac gtcactgacg
      121 gctgctacaa tgttcgtaca gaacatccgt gcgccttgtc gtgcgtgcaa ccttctcccc
      181 tccccgcgtc ggggaggggc cgggggaggg gtttgggggt tagcagcatg tccctcgcag
      241 cattcctcga tcggctgaac cgcgaagagc gctgggcgtc gtgtatcgcg gcctggaaga
      301 ccgtcccagc tcaggaggcg atcacggccc cgatcccggg ggcgctcgat ccgcgcctgc
      361 gccgggcgtt gatcgcacgc ggtgtcgatg agctctacgt ccaccaggcc gacgcgttcg
      421 atgcggtcat caccggcaac aacacggtcg tcgtcacgcc gacggcatcc ggcaagacgc
      481 tctgctacaa cctgccggtc ctcaatcatc tactcagtga gccgacggcg cgggcgatgt
      541 acctctttcc aaccaaggcg ctggcacagg accaatacgc cgggttgcag gcgctactgg
      601 atgccgccgg ggccgacatc aagacctggc cgtacgacgg cgacaccccc gccgccgagc
      661 ggcgactcat ccgtcaggcc ggccacatca tcatcaccaa tcccgacatg ctgcattcgg
      721 cgatcctgcc gcaccacacc aagtggcacg cgctgttcga gaatctcaag tacgtcgtca
      781 tcgatgagat gcacggctac cggggtgtct tcggctcgca cgtcgccaat gtcatccggc
      841 ggctgaagcg gatctgccgt cactacggat cgtcgccgcg cttcattctc gcctcggcga
      901 cgatcgccaa ccccggtgag ctggccgaga agctgatcga gttaccggtc gaggtcatca
      961 cccgcaacgg cgcgccgcgc ggtcggaagg acttcatcct ctacaacccg cccgtcgtca
     1021 accgcgcgct cggcatccgc cgctcggcca ttctggaggc cagcggtctc gccagcgagc
     1081 tgatccgcaa cgacatccac acgatcgtct ttgcccgcgc ccgcgtgacg gccgaggtgc
     1141 tgctgaccta cctgcgcgag gcgctgccgc ccaagatcgg acaaagcaac gcgattcgcg
     1201 gctatcgcgg cggctacctg cccaaccagc gccgcgagat cgaggctggc ctgcgcgatg
     1261 gccggatcaa gggcgttgtc tcgaccaacg cgctcgaatt gggcgtcgat atcggctcgc
     1321 tggatgcggc tgtcatgacc ggctaccccg gcacgatcgc cagcgcctgg cagcaggccg
     1381 gccgggccgg acgccgagag agcatgtcgg ccgcgatcat ggtcgccagc tcgtcgccga
     1441 tcgaccagtt catcgtcaac caccccgagt acttcttcga gggcagcccc gagaacggcc
     1501 tgatcaaccc cgacaacctg ctgatcgtcg tcaaccacat caagtgcgcc gccttcgagc
     1561 tgcccttcaa gcgcggcgag atgttcggca cctacgagcc gaccgacgcg ctcgactacc
     1621 tggccgagat cgacatcctt cattacgtca acaacacctg gcactggatg tccgactcct
     1681 accccgccga ggacctcagc ctgcggacgg ccgcccgcga gaacgtcgtc atcatcgaga
     1741 agggagacgg acgcccgcgc gtgatcggcg aggtggacgc gttctcggcc ccgatgctgg
     1801 tccacaccga cgcgatctac atccacgatg gccagcagta ccacgtcgac gagctggact
     1861 gggaggagaa gaaggcctac gtcaagccgg tcgatgtcga gcactacacc gacgccagcc
     1921 tttcggtgcg ggtcgaagtg atcgagcagt tctccgacgg cgacccggcg gccgagcgcc
     1981 accacggcga ggtgctggtc ggcgcgatcg tgacccagtt caagaagatc cgcatgcaca
     2041 cccacgagaa cctgggctgg ggcaaggtct tcctgcccga gtcgcagatg cataccagcg
     2101 cctactggac cagcctgccg gcctacctct ccgaacgaat ggccgagccg gtcctccacg
     2161 gcgccctttc cggcctggcg aacctgctcg gcaacatcgc cccggtctac ctgatgtgcg
     2221 acccgcgcga cctcggcgtc tacgcccagg ttcgcgcccc gttcaccggc gccgcaaccg
     2281 tcttcatcta cgacaaggtc cccggcggca tcggcttctc cgagaaactc tacgaggccc
     2341 accacctgct gctgaaagcc gccctcgacc acgtcacccg ctgtccctgc caggacggct
     2401 gccccagctg cgtgggagta cgccccagcg acgaatcact cccgttcgcc aagaagacga
     2461 cccgacagct gctggaggta ctgcacatgc gaactccacg ctcgtacggt caacactatc
     2521 cagagtagca tccggtgcgc tatcctccga tttgggaggc tgatggcaat ggcgagaacg
     2581 tccatgacag ttgaacaggt cttgccgctg cttgccgcaa caccgcagcg gatcgtcgcg
     2641 gcgacggacg ggctggcgcc gatgcagttg cggaccgcgc cggagcacga cgactggtcg
     2701 gcgaacgatg ttctctccca tctgcggtcg tgtgccgatg tctggggcga ctgcatcgca
     2761 accatcctcg ccgaggagcg gccaacgata cgggccatcg atcccagaac ctggatgaag
     2821 cagacgaatt atcctctgct ggagtttcag ccgtcgttgc aggaatttct cgcccagcgc
     2881 gccgacctgc tggcggtgct ggaacggttg acgcctgacg actggtcacg cggtgcgacc
     2941 gtgaccggtg cgggagcgcc gcttgagcga acggtgctgt cgtacgcgca gcggctggca
     3001 cggcacgaac ggtcgcacat caagcagatc caacgcatcg tcagcacgtt ccagacgtag
     3061 tagcgtcagc cggctcgcgg cgccactcgt gatcgcttgg caaatccaga cgcctccgct
     3121 atgctgcaag cgaacagatg ttcgatgtac gtacggaggt gaacgtgggc aactgggtag
     3181 cgattggtcc ggccggaacg cgcggatatc tggcagtgcc ggagagcggc tccggtcccg
     3241 gcctgctggt gctgcatgcg tggtgggggc tgacatccgc ctttaccgac atctgcgacc
     3301 aactggcagc ggaggggtat gtcgcgctgg cgccgagcct gtacgccagc ggggcgacgg
     3361 ccgagacgat cgccgaagcc gaggcgctga tgagctccca cgacgagggg accgaatcga
     3421 aggcgatcgc cctggccgcg acggaccagc tcctcagctt gccggccgtg tctggatcca
     3481 agatcggcgt catcggttac tcgatgggcg cctactgggc gctgcaactc tcgcaactgc
     3541 gaccggagaa cgtcagcgcg gccgtgacgg tctacggcac gagcgacggc gatttcggcg
     3601 ccgcgaaggc cgcctacctc ggtcacttcg ccgagcatga cgactacgag ccgctggagg
     3661 gagtccgggc gctggaggag cagatccgcg acgcaggctg cgaggtcgcg ttcttcatct
     3721 atcccggcac cagccactgg ttcgtcgaac ccaaccagcc gaacgcctac aacgccgagg
     3781 cagcgcaatt ggcgtgggat cgcacgctcg cgttcctgag ggccaagctc gcctgatccg
     3841 gtttcgcaat caattccaca tccccaatcc ggtaggggtg attttgcaaa atcgcccttc
     3901 tccgagcgcc ggggatctga aagggtgatt ttgcgaaatc acccctaccg gattggggat
     3961 tgaatatgag ggattgtccc caggaacatg gcggcatgtt gcctccccat agagacgacc
     4021 tgccgcaaaa gcgttcgaat gctccgagct atgccgatcg caacgtcgct cgattcgtcg
     4081 cttcggccca gtcccagagg cccaggtcgg ggatcgcgat gccgagcgat gtcaggatcg
     4141 tgcagatgtg ggcgcggtgt tcggtgccgt ggtgcagtgc ctgcatcagc accacaccag
     4201 ccgggacatc gcggacggcg ccgtcttccc agttgatcgc gaaggtgcgc tcggcgtcaa
     4261 agg
//
LOCUS       DASUGJ010000758         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1654939, whole genome shotgun sequence.
ACCESSION   DASUGJ010000758 DASUGJ010000000
VERSION     DASUGJ010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654939"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VFV93_17140"
     CDS             complement(<1..1127)
                     /locus_tag="VFV93_17140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5167134.1"
                     /translation="MTLLVQAAEVQYAHGGNQIFDGVSFEVRAGDRLALIGENGAGKS
                     TLFRLMAREILPQGGAVTHKRGLTLEYLSQHSTLDPALTVHEAIALAAGDPAALEGQL
                     RDLEAKMAEPLDDDDLSAVLEEYSAVLERFEGAPGQDLGARIAQILTGLGLPQERWDT
                     QVGNLSGGEKKLVALAGFLIEEPELLLLDEPDNHLDESARGWLEGYLRERRGAVAVIS
                     HDRYFIDRVANRIFELEDGRITGYPGNYSAFLTLKRERLERDEQLYDMRQRELKKLKA
                     SAEQLTQWAKQNPKFAARAGNRWRNFEIEKERLENTPTPILDRKRIEVDFSAGRGSSL
                     VLEIKGLAKAYGEREIFKPFDFTMMHGERVGIVGGNGAGKTT"
     gene            1949..3736
                     /gene="recN"
                     /locus_tag="VFV93_17145"
     CDS             1949..3736
                     /gene="recN"
                     /locus_tag="VFV93_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX5167135.1"
                     /translation="MLIELTIRNFAVIQETRIELARGLNALTGETGAGKSIVIDALSA
                     VLGARTSAEFVRSGERGAYVEAVFDISDLPDPAPVQAMLAELGVEVQPDEPLVLSRDV
                     SASGRSTARVGGRTVTAAALAQLGELLVDIHGQSDHLSLLRPASQLDLLDRFAGTLPQ
                     RDEVATAYRDWQALRRRVHDFDVEQRTLAQRLDLLRFQSEELGNAGIRPGEDEQLQQE
                     RTVLANAERLIRSAAEAESALNGDATGATLDAGALDRVRAATRSLDDAIQLDPTLEPT
                     LTKLNDALFALEDVVVELRDYAERTELDPLRLEQVDERLDLLRRLKRKYGPELSDVIE
                     HAEQIQQELESIEAGEHDIDALRSEEASQRSQLARLAWQLSEQRKAAATTLAKRVEQA
                     IAELNMGRASFEVRFDTTPSPDGVPGPDAGDPVAIDATGYDRIAFYLAANAGEELRPL
                     ARVASGGETARLMLALKSILSDADDTPILVFDEVDVGVGGRSGQVVGEKLWSLTDKHQ
                     AIVISHLSQIAAFADRHLVLVKDEVDARTVTTARPVEGDERVEELAAMLDGLPPTPES
                     RANAGALLQRVNAWKDAARGGQSRPKKRR"
     gene            3834..4124
                     /locus_tag="VFV93_17150"
     CDS             3834..4124
                     /locus_tag="VFV93_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX5167136.1"
                     /translation="MRMIQQMQNRIQKMQEELGQTVVEGTAGGGMVTVQVTGLKEVQS
                     VKIAPEAVDPDDLEMLEDMIVAAINDAMQNAETISADKMSAITGGIKIPGLT"
BASE COUNT          742 a         1390 c         1360 g          708 t
ORIGIN      
        1 gtcgtcttgc cggcgccgtt gccaccgacg atgccgaccc gctcgccgtg catcatcgtg
       61 aagtcgaacg gcttgaagat ctcccgctcg ccatacgctt tcgccagccc cttgatctcc
      121 agcaccagcg acgatccacg cccggccgag aagtcgacct cgatccgctt gcggtcgaga
      181 atcggcgtcg gcgtgttctc cagccgctcc ttctcgatct cgaagttgcg ccagcggttg
      241 ccggcccggg ctgcgaactt cgggttctgc ttcgcccatt gagtcaactg ctcggcgctg
      301 gccttgagct tcttcagctc gcgctggcgc atgtcgtaga gctgctcgtc ccgctccagc
      361 cgctcgcgct tgagcgtcag aaaggccgag tagttgccgg gatagccggt gatccggccg
      421 tcttcgagct cgaagatgcg gttggcgacc cggtcgatga agtagcggtc atgcgagatg
      481 acagcaaccg ccccgcgccg ctcgcgcagg tagccttcga gccagcctct ggccgattcg
      541 tcgaggtggt tgtccggctc atcgagcagc agcagttccg gctcctcgat caggaatccg
      601 gccagcgcga ccaacttctt ctcgccgccg ctgaggttgc cgacctgcgt gtcccagcgc
      661 tcttggggca ggccgagtcc ggtcaggatc tgggcgatcc gggctccgag atcctgcccg
      721 ggtgcgccct cgaatcgttc gaggacagcg ctgtactcct ccaggaccgc gctcaggtcg
      781 tcgtcgtcga gcggttccgc catcttggct tcgaggtcgc gcaactgccc ttcgagcgcg
      841 gccgggtcgc cggcggccag cgcaatcgcc tcatggacgg tcagtgccgg gtcgagcgtc
      901 gagtgctggc tcaggtattc gagcgtcaga ccgcgcttgt gcgtcacggc gccgccctgc
      961 ggcaggatct cgcgtgccat cagccggaaa agcgtcgact tgccggcgcc gttctcgccg
     1021 atcagcgcca aacgatcgcc ggcgcggacc tcgaagctga cgccgtcgaa gatctgattg
     1081 ccgccgtggg cgtattgcac ctcagccgcc tgcaccagca gcgtcattcg gatcgtcctc
     1141 ttcctcaacc acagcccatc gaccgtcgcg gcgaataccg acagcctaac acgccggagc
     1201 ccagcggtcc ctgctcgtct gcgctgttgc cggacggcga ttggttcagg gaaggagtgg
     1261 agagacgact cagggcgacg ccggctggcg tcgccctgag cacaggtggg gtgaggtggg
     1321 aatgggagga agggagtagt caggggcgaa gcgcgctgtg cagggccacg cgtcgccgcc
     1381 actcctactt cttgaagagc gtcttccagt tgcgctgccc gcgcgacttg gccaggttct
     1441 ggtgggtgta ctcaaccatc ttcgacatcg tgtacggatc catcagagcg ttcccttcgt
     1501 gtatgcccaa acggcgaaat aacgtgcgat cagcgacggg tgccaccggt ggcggacgcc
     1561 agttggttga tccgaaccgg catctgccag cgatttatgt actgttctgc tttgatctga
     1621 ccaactcggc tcatgtctgc ctccttctga ttgagagatt agcatgacat ctcgctgttg
     1681 tcaactgttt tgtgtcgatt gttcagaatc agtgcagttt caataagaat caggcgaaga
     1741 cgctgctgac gccgtccaac tgggacgcgg cccaggggca gcaccggatc cgcagaacgt
     1801 tcgtggtatc gcattgcgac ggccccgagc atcgagtgag gagccgcgcc gcaccgccac
     1861 gcaggagccg accaacgcgc tactattagt gtcgtcatcc gggcggcaac caactgcgat
     1921 acgtgctgat gttgaagagt agaacgctgt gctgatcgaa ctgacgattc gcaacttcgc
     1981 agtgattcag gagacgcgga tcgagctggc gcgcggtctg aacgcgttga ccggcgagac
     2041 cggcgccggc aagtcgatcg tcatcgacgc gctcagcgcg gtgctgggcg cacgcacctc
     2101 ggccgaattc gtgcgcagcg gcgagcgtgg cgcctatgtc gaggctgtct tcgacatcag
     2161 cgacctgccc gaccccgcgc ccgtccaggc catgctggcg gagctcggtg tcgaggtcca
     2221 accggacgag ccgctcgtcc tctcgcggga tgtcagcgcc tcgggccgtt cgacggcccg
     2281 ggtcggtggc cggaccgtga cggccgccgc gctcgcccag cttggggagc tgctggtcga
     2341 tatccatggc cagagcgacc atctctcgct cctgcggccg gcctcccagc tcgatctgct
     2401 cgatcgcttc gccgggacac tcccgcaacg cgacgaggtc gcaacggcat accgcgactg
     2461 gcaggcactg cgcaggcgtg tccacgactt cgacgtcgag cagcgcacgc tcgcgcaacg
     2521 gctcgatctg ctgcgcttcc agtccgaaga gcttggcaac gccggcatcc gacccggcga
     2581 agacgagcag ctccagcagg aacggaccgt tctcgccaac gctgagcgtc tgatccggtc
     2641 ggccgccgaa gcggaatcgg cgctgaatgg cgacgcgacc ggcgcgacgc tcgatgccgg
     2701 cgcgcttgac cgggttcggg ctgcgacccg ctcgctcgac gacgccatcc agctcgatcc
     2761 gacgctggaa ccgacgctga cgaagctcaa cgacgcgctc ttcgcgctcg aagatgtcgt
     2821 cgtcgagcta cgcgactacg ccgaacggac agaactcgat ccgctccggt tggaacaggt
     2881 cgacgaacgg ctcgacctgc tgcgccggct aaagcgcaag tatgggccgg aactcagcga
     2941 cgtgatcgag cacgccgagc agattcagca ggaactggag tcaatcgaag ccggcgagca
     3001 cgacatcgac gcactgcgct ccgaggaagc gtcgcagagg agtcaactcg cccggctggc
     3061 atggcaattg tctgagcagc gcaaggcggc cgcgaccacg ctcgccaagc gggtcgagca
     3121 ggcgattgcg gagctgaaca tgggccgcgc cagcttcgag gtccggttcg acacaacacc
     3181 gtcccctgac ggggtaccgg ggccggacgc tggcgatcca gtcgcaatcg acgcgaccgg
     3241 ctacgaccgg atcgcgttct atctcgccgc caacgccggc gaggagctcc gcccgctggc
     3301 gcgggtcgcc tccggcggcg agacggcgcg gctgatgctg gcgctcaagt cgattctctc
     3361 cgacgccgac gacacgccaa ttctcgtctt cgacgaggtc gatgtcggcg tcggtggccg
     3421 cagcggtcag gtcgttggcg agaagctctg gagcctgacc gacaagcacc aggcgatcgt
     3481 catctcgcat ctctcgcaga tcgccgcctt cgccgaccgg cacctggtgc tcgtcaagga
     3541 cgaggtcgat gcccggacgg taacgaccgc ccgtcctgtc gagggcgacg agcgcgtcga
     3601 ggagctggcc gcgatgctcg acggcctgcc accgacgccg gagtcgcggg ccaacgccgg
     3661 cgcccttctc cagcgcgtca acgcctggaa ggacgctgcc agaggcggcc agagccggcc
     3721 gaaaaagcgc cgctgatata atcgccatca gcccggcaga acgccggttg ccgtacgcac
     3781 atcgaatcct gaattcatgt ctccggaaag gaccgccaac cgtgcagccg aacatgcgca
     3841 tgatccagca gatgcagaac cggatccaga agatgcagga agagctcggc cagacggttg
     3901 tcgagggaac ggccggcggt gggatggtga cagtgcaggt aaccgggcta aaggaagtcc
     3961 agagcgtgaa gatcgccccg gaagccgtcg accccgacga cctggagatg ctcgaagaca
     4021 tgatcgtcgc cgccatcaac gacgccatgc agaacgccga gacgatctcg gccgacaaga
     4081 tgtccgccat caccggcggg atcaagatcc ccggcctgac gtagaacaat caccctggat
     4141 gtgtcgcgta ggggcgatgg ccgcagcctc gcccttgttc ggcacgcgtc aggcagggcg
//
LOCUS       DASUGJ010000759         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_123837, whole genome shotgun sequence.
ACCESSION   DASUGJ010000759 DASUGJ010000000
VERSION     DASUGJ010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123837"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..873
                     /locus_tag="VFV93_17155"
     CDS             46..873
                     /locus_tag="VFV93_17155"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5167137.1"
                     /translation="MDTGDIAPKPAHLGPQYGAQFGDDSIVRAYRHRPPYPIELFGIL
                     ASLLPSVPGAILDAGCGNGDLTLGMLANLPGIERLDAIDLSAGMIEAGRQRPGGDEPR
                     LRWQVAPLETATLSPPYGLVTAGESMHWMDWEVVLPRFRDALLPGGVLAIVGRGPRPS
                     PWTDDLIRIVQRHSTNRDFQPYDLIDELASRRLFRVLGRKTTAPVICTQTIDDHIEFW
                     HSRNGLSRDRMTTAGAFDREMRALLAGYSHRETVTFDVVGSVTWGLPAPDPGQRENA"
     gene            complement(835..1500)
                     /locus_tag="VFV93_17160"
     CDS             complement(835..1500)
                     /locus_tag="VFV93_17160"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX5167138.1"
                     /translation="MIRAVMFDMDGVLVDSEPAHLEMTRETLRHFDLPLPDDDDWEAI
                     FFGRPDRDGLAEWFRRHHLTADIRAIMADKLRRFEARFTELVAPFDDGQWLARELHRH
                     GLPLALVTGARRDEANLVLSRFNLADVFSVSISSDESIAGKPDPRPYLMGAEALGVDA
                     SACVVIEDAIPGLRAAEAAGATPIVVDRLGQPQRFAPLTPVTTLNEQVLSRILALAGI
                     GCR"
     gene            complement(1906..2331)
                     /locus_tag="VFV93_17165"
     CDS             complement(1906..2331)
                     /locus_tag="VFV93_17165"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5167139.1"
                     /translation="MAERVRVTAGGVSSLVRKGGRLLAVLALGPALLVGCSIEDIRGG
                     EEAATPTTQAVAAATPTGATPLKIVTPTPFVAGATTTAGLTPTVPGEHPDVYVVQEND
                     TLYGIAVRFNVELDDLIALNGLSDPNDIRIGQELQIPKE"
BASE COUNT          416 a          830 c          785 g          395 t
ORIGIN      
        1 ggccacgaac gagcctgata taccgatcgg acggaagaat gcacaatgga cactggagat
       61 atcgccccga aaccagccca ccttggcccg cagtacggcg cgcaattcgg cgatgacagc
      121 atcgttcgtg cgtatcgcca ccggccgccg tacccgatcg agttgttcgg catcctcgcg
      181 agcctgctcc catcagtccc aggagcgatc ctcgatgccg gttgcggcaa tggcgatctg
      241 acgctgggga tgctcgcgaa cctaccgggc atcgagcggc tggatgccat agacctctcg
      301 gccgggatga tcgaggcggg ccggcagcgg cccggcggcg acgaaccacg cctgcgctgg
      361 caggtcgcgc cgttggagac ggcgaccctc tcgccgcctt acgggctcgt gacggctggc
      421 gagagcatgc actggatgga ctgggaggtt gtcctgccac gcttccgcga cgcgctgttg
      481 cccggtggcg tgctggcgat cgtcggccgt ggcccgcggc cctcgccgtg gaccgatgac
      541 ctgatccgga tcgtccagcg ccactcgacc aaccgcgact tccagcccta cgacctgatc
      601 gatgagctgg ccagccgtcg cctcttccgc gtcctcggcc ggaagacgac cgcgccggtc
      661 atctgcaccc agacgatcga cgaccacatc gagttctggc actcgcgcaa tggtctctcc
      721 cgtgaccgga tgacgaccgc cggcgcgttc gaccgcgaga tgcgtgcgtt gctggccggc
      781 tacagccatc gggaaacggt gacgttcgac gtcgttggca gcgtgacctg gggactaccg
      841 gcacccgatc ccggccagcg cgagaatgcg tgacagaacc tgctcgttga gcgtggtcac
      901 tggcgtcagc ggcgcgaagc gctgcggctg gcccagccga tcgacgacga ttggtgtcgc
      961 gccggccgct tcagccgccc gcaagccagg gatagcgtcc tcgatcacga cgcaggcgct
     1021 ggcatcgaca cccagcgcct cggcgcccat caggtacgga cgtgggtcgg gcttgccggc
     1081 gatcgactcg tcggagctga tcgagacgct gaatacgtcg gcgagattga agcgtgacag
     1141 gaccagattc gcctcgtccc gccgcgcgcc ggtcaccagc gccagcggca ggccgtgccg
     1201 gtgcagctcc cgcgccagcc actgcccgtc atcgaagggg gcgaccagct ccgtgaagcg
     1261 cgcctcgaag cgccgcagct tgtcggccat gattgccctg atgtcggccg tgaggtgatg
     1321 ccgccggaac cactcggcca gcccgtcccg atccggccgg ccgaagaaga tcgcctccca
     1381 gtcgtcgtca tcgggcagcg gcaggtcgaa gtgacgcagc gtctcacgcg tcatctcaag
     1441 gtgggccggc tctgaatcga ccagcacccc atccatgtcg aacatcaccg ctctgatcat
     1501 ctgctccacg tccccaacgc cggattccgg gcaaaacgaa agccggccac tgtgggccgg
     1561 cttcctggtg tttcgggcac tcaccccgaa ggggacagac tagctggccg cctccgaggc
     1621 gatgcccgta agaccatgac tactcatgat ttggcatcac ctccttcgcc ctgaacggtg
     1681 tcttcactaa gggcggaccc gtgaagcaat tcacgggacg ggtcaacgcg agaagcatac
     1741 cagcggccag agacagtgtc aacgaccgtg agtcaccgct gattgttagc cagctgctat
     1801 ccgtcaccac tgcattcgcg taggggcgac ggccgccgtc tcgcccgatc ttggtcgcac
     1861 ggggcagggc gaggctgtgg ccgtcgcccc tacgcgaggg tggaattact ccttcggaat
     1921 ctgcagttcc tggccgattc ggatgtcgtt gggatcggag aggccgttga gggcaatcag
     1981 atcatccagc tcgacgttga agcggacggc gatcccgtag agcgtgtcat tctcctggac
     2041 gacgtagacg tccggatgct ctccggggac ggtcggcgtc agcccggcgg tcgtcgtcgc
     2101 accggcgacg aatggggtcg gcgtgacgat cttcagcggt gtcgcgccgg tcggggttgc
     2161 agcggcaaca gcttgcgtcg tcggcgtcgc ggcctcttcg ccaccacgaa tgtcctcgat
     2221 cgagcaacca accagtagcg ccggacccaa cgccagaacg gccagcaggc gcccaccctt
     2281 ccgcaccagc gacgatactc cgccggccgt cacccgcacc cgctctgcca cgtcagatcc
     2341 cctccccaga ctcactcctg cgtggctcga tgatacagga ttgcggcggg cgggacgcgg
     2401 gacgcgggat tcggggcggg gtcaca
//
LOCUS       DASUGJ010000760         3342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1513471, whole genome shotgun sequence.
ACCESSION   DASUGJ010000760 DASUGJ010000000
VERSION     DASUGJ010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513471"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="VFV93_17170"
     CDS             complement(<1..1404)
                     /locus_tag="VFV93_17170"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/3-dehydroquinate
                     synthase"
                     /protein_id="HEX5167140.1"
                     /translation="MTRLPERVILIGPSGSGKSSIAVKLAASLGYAAIDCDDMIVERI
                     GMPIGAFFARFGEPAFRAIEADVVAEACRRSQVVIATGGGAILSASNRTTMRPNSLIV
                     SLTARPETLVERVRRHAEASGNLAERPLLAGDAEDRMRAMLAKRGPLYATADVQIDTD
                     GLVAEQVLKRVLTAIQIDDAVAIPCLSLATGGDRSDIYVGEGLSGAAGELAARRWPKA
                     RRAWLVADDNVGPRWGDWVAASMRGAGIDVSVLTIAAGEGSKDLRVVERLCTEMTAAG
                     VTRRDLVVALGGGVTGDLAGFVASICLRGLSLMQVPSSLLAMVDSSVGGKTGVNMPAG
                     KNLVGAFYQPGMVLVDPRLLDTLPQAEYRSGMAEVIKHALIQPSTPLGGTDLLESLSA
                     LDLDPLPSETIAGILERNVAIKHSVVQADERESGLRMILNFGHTAGHAIEADGYRYRH
                     GEAVGLGLIVASRIAASL"
     gene            1649..3034
                     /locus_tag="VFV93_17175"
     CDS             1649..3034
                     /locus_tag="VFV93_17175"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5167141.1"
                     /translation="MPRILVADDDAGIRTLLRELFEDEGYDVDEAVNGSAVVDAFQKA
                     DSLHELTIMDVKMPEMSGLDVLRKLSDKGDGPLPVIVMTAFGSASIAIEAMQLGAYDY
                     VTKPFDLDDVLLRVQRYFEHQELAREVQKLRRLAERDPNEYIIGNSPAMQDVFKTIGR
                     VARTEATVLITGETGTGKELVATTLHRNSGYAHGPLIKVNCAALPETLLESELFGHEK
                     GAFTGAIAQKKGHFERANRGTIFLDEIGEMTLATQKKLLRVLQEREFERVGGTVPVKV
                     DTRVVTATNKNLAQEVREGQFREDLYYRLNVIVVHLPALRERREDIPLLIEHFLDKHR
                     VSSSAQPSRISTGAMRMLQDHDWPGNVRELENTVERAIVLAGGGVITEDHITFSSTDQ
                     GHFVDISAKVRNSTPIREVLQEVERQMIREALRQANGDRVTAASLLGYDMAELSEKLS
                     DYGISGDASEV"
     gene            complement(3001..>3342)
                     /locus_tag="VFV93_17180"
     CDS             complement(3001..>3342)
                     /locus_tag="VFV93_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167142.1"
                     /translation="EMIPVVGAGLFWRTVARDLAAMMPFAAGAVPKVAIAFAGTFAAG
                     MAAHLYYQEGKRVDRQRMRQYYRDALQQLRDRPDLLKSLPIPNRLRRDDDADGVIIDV
                     DYTSDASPEMP"
BASE COUNT          632 a         1118 c         1043 g          549 t
ORIGIN      
        1 gagcgaggcc gcgatccggc ttgcgacgat caggccgagc ccgacggcct ccccgtggcg
       61 gtagcggtag ccatccgcct cgatcgcgtg tccggcagta tggccgaagt tgaggatcat
      121 ccgcagcccg ctctcacgct cgtcggcctg gacgaccgag tgcttgatag cgacgttgcg
      181 ttcgagaatg cccgcgatcg tctcactcgg taacggatcg agatcgagcg cggagagcga
      241 ttcgagcaga tccgtcccac cgagcggcgt cgatggctgg atcagcgcat gcttgatgac
      301 ttcggccatc cccgagcgat actcagcttg tggcagcgtg tcgagcagac gcggatcaac
      361 cagaaccatc cctggctggt agaacgcgcc aaccagattc ttgccggccg gcatgttgac
      421 gcccgtcttg ccaccgacac tcgaatcgac catcgccagg agcgacgacg gcacctgcat
      481 cagcgacagc ccgcgcagac aaatgctggc gacgaatccc gccagatcac cggtcacgcc
      541 tccgccgagc gccacaacga gatcacgtcg ggtcacgccc gctgccgtca tctcggtaca
      601 cagccgttcg acgacgcgca gatccttgct gccctcgccg gccgcgatcg tcagcaccga
      661 gacgtcgatg cctgccccac gcatcgacgc agcgacccag tcgccccagc gcgggccgac
      721 gttgtcgtca gcaaccagcc aggcgcgccg cgccttcggc cagcgccggg ccgcaagctc
      781 gccggcagcg cctgacaatc cctcaccaac atagatatct gatcggtcgc cgccggtcgc
      841 cagcgacagg cacggaatcg ccactgcatc atcgatctgg atcgcggtaa gtacgcgttt
      901 caggacctgc tcggcgacga gcccatcagt atcaatctgt acgtcggcgg ttgcatacag
      961 tggaccgcgt ttggcgagca tcgcccgcat gcggtcctcg gcatcacctg ccagcagcgg
     1021 gcgttctgcc agattgccac tcgcttcagc atggcggcgc acgcgttcga ccagcgtctc
     1081 cggccgggcc gtcagcgaga cgatcaggct gttcggacgc atcgtcgtcc ggttggatgc
     1141 ggagagaatc gcgccgccgc cagtcgcgat taccacctgc gatcggcgac aggcttcggc
     1201 gacgacatcg gcctcgatcg cgcggaacgc cggctcgccg aaacgggcga agaacgcgcc
     1261 gatcggcatc ccgatccgct cgacgatcat gtcgtcgcag tcgatcgcag cgtagccgag
     1321 actggctgcc agcttgacgg cgatactcga cttcccggac ccgcttggtc cgatcagaat
     1381 gacgcgttcg ggcaggcggg tcacactcgt ttgtctccat tgctcggcgt ttagcggtag
     1441 ccgttgtcgt atgggagtat agtcgcaccg gcacccaaac ggcccgatca agagccgctg
     1501 gaatcggcgc tgggccgccg tgtcgcttat acttgccggc acgagtgtca atctgacacc
     1561 gatcccgttc gcatccggtc gcacacaggt gcgatagcgg cgtccgcacg aatccggcgc
     1621 cgtttcctct gagaggttgg ttcaatctat gccgcgcatt ctggtcgcgg atgacgatgc
     1681 ggggatccgc acactcctgc gcgaactctt cgaagacgaa ggctacgacg ttgacgaagc
     1741 cgtcaacggc agtgccgtcg tcgacgcgtt tcagaaggca gactcgctcc acgagctcac
     1801 catcatggac gtcaagatgc cggagatgtc cggcctcgat gtcctgcgca agctgtccga
     1861 caagggcgac ggcccgttgc cggtcatcgt gatgacggcc ttcggcagcg cctcgatcgc
     1921 gatcgaggcg atgcagctcg gcgcatacga ctacgtcacg aaaccgttcg atctcgacga
     1981 tgtcctgctg cgcgtgcaac gctacttcga gcatcaggaa ctggctcgcg aggtccagaa
     2041 gctgcgacga ctggccgagc gcgacccgaa cgaatacatc atcggtaaca gcccggccat
     2101 gcaggatgtc ttcaagacga tcggtcgcgt cgcccgaact gaagcgaccg tcctgatcac
     2161 cggcgaaacg ggaaccggca aggaactggt cgcgacgaca ctccaccgca actcaggcta
     2221 tgcgcatgga ccactcatca aggtcaactg cgccgcgctg ccggagacgt tgctcgagag
     2281 cgagctgttc ggccatgaga agggcgcctt caccggcgcg atcgcccaga agaaggggca
     2341 tttcgagcgc gccaaccgcg ggacgatctt cctcgatgag atcggcgaga tgacactggc
     2401 gacccagaag aagctgctgc gcgtccttca ggaacgcgag ttcgagcgcg tcggcggtac
     2461 ggtgccggtc aaggtcgata cgcgggtcgt gacggcgacc aacaagaacc tggcccagga
     2521 ggttcgcgag ggacagttcc gcgaggacct ctactacagg ttgaacgtga tcgtggtcca
     2581 cctgccggcg ttgcgcgagc gccgcgagga tatccctctg ctgatcgagc acttcctcga
     2641 caagcaccgc gtctcgtcgt cggcacaacc gtcgcggatc tcgaccgggg cgatgcggat
     2701 gctgcaggac cacgactggc ccggcaacgt ccgcgaactg gagaacaccg tcgagcgggc
     2761 gatcgtcctg gccggcgggg gagtcatcac cgaggaccac atcaccttct cgtccaccga
     2821 tcagggtcat ttcgtcgata tctcggccaa agtccgcaac tcgacgccga tacgcgaggt
     2881 gttgcaagag gtcgagcggc agatgatccg cgaggcgctg cggcaggcca acggcgaccg
     2941 cgtaacggcc gcctcgctgc ttggctatga catggcagag ctgagcgaga agctgagcga
     3001 ctacggcatc tccggcgacg cgtctgaggt gtaatcgacg tcgatgatga ccccatcggc
     3061 gtcatcatcg cgtcgcaggc ggttcgggat cggcagggac ttgagcaggt ccggccggtc
     3121 ccgcaactgc tggagcgcgt cccggtagta ctgccgcatc cgctgcctgt cgacccgctt
     3181 gccctcctgg taatacaaat gcgccgccat cccggccgca aacgtaccgg cgaatgcgat
     3241 cgccactttc gggaccgcgc cggccgcgaa cggcatcatc gccgccaggt cgcgggcaac
     3301 cgtccgccag aacagcccgg cgccgacgac cgggatcatc tc
//
LOCUS       DASUGJ010000761         5118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_959984, whole genome shotgun sequence.
ACCESSION   DASUGJ010000761 DASUGJ010000000
VERSION     DASUGJ010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959984"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFV93_17185"
     CDS             <1..246
                     /locus_tag="VFV93_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEX5167143.1"
                     /translation="VIIVAAGMDGALPSVVSGLVDIPVIGLPTSVGYGVAAGGQAALM
                     TMLSTCAPGMAVVNIDNGVGAGATAALIARRAHQPRD"
     gene            complement(231..1199)
                     /gene="meaB"
                     /locus_tag="VFV93_17190"
     CDS             complement(231..1199)
                     /gene="meaB"
                     /locus_tag="VFV93_17190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX5167144.1"
                     /translation="MTATGTDRLIERFRNRERPALARLVSYVENERPAGTRAMAELYR
                     DTGNAYVVGITGPPGAGKSTLTNAVIREYRKRGKTVGVLAIDPSSSLTGGATLGDRIR
                     MLENYDDDGVYIRSMATRGEMGGLSMAVGAVTHLLDAYGFDIIIVETVGVGQDEVDVA
                     ELADTVLLLQVPGLGDSIQTIKAGVLEIADILVVNKADIKGAENVVRDLRNMLRLGEH
                     GPWLPPIVRTVAVYEEHIDELLNTIDKHRAHLEASGEGERRRERRERREVLRLAQNWL
                     GARLSETLSEPSMEPVLADLRHRDVNPFEVAEALARQTATRIQSRG"
     gene            complement(1196..1621)
                     /locus_tag="VFV93_17195"
     CDS             complement(1196..1621)
                     /locus_tag="VFV93_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX5167145.1"
                     /translation="MIETQARPRPIRVLIAKPGLDGHDRGAKVMARALRDAGMEVIYT
                     GLFQTPEMIARAAVDEDVDVIGLSILSGAHRALFPKIFSELEGAGLTDVLVVAGGTIP
                     NEDVAALKELGVAEVFGPGTPLESAVRFIREHAPQRGDE"
     gene            complement(1673..3322)
                     /locus_tag="VFV93_17200"
     CDS             complement(1673..3322)
                     /locus_tag="VFV93_17200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX5167146.1"
                     /translation="MSQRYSTDFHDPRYQGAPPEEREFTTLSGEPVQPLYTRDDLDRV
                     GFDEAKDLGRPGQPPYTRGIHESMYRGKLWTIRQFAGFGSAEETNARFRYLLEHGETG
                     LSIAFDLPTLYGRDTDDPMSHGEFGKCGVAVSSLADMEILLDGIPLDAVTTSMTINGP
                     AAVIWAMYIAAAEKQGVPRAKLRGPLQNDILKEFTAQNEYIFPPKPSMKLVTDTIEFS
                     THQMPLWNSISISGYHIREAGATATEELAFTLADGIAYVEAALERGLDIDAFAPRLSF
                     FFNCHNDFFEEIAKFRAARRIWYRVMRERFGAKNERSCWMRFHTQTAGCSLTAQEPEN
                     NVIRTTIQALAAVLGGTQSLHTNSLDEALALPSDWAVRIAVRTQQIIAHESGVANSVD
                     PLGGSYMVETMTSEMEDRVQTIFDEIDALGGVVPAIEGGYFQRRISDSSYAYEQALAE
                     QRKIVVGVNAFRSEEEPSIPILEMDPQGEGRHLERLARVRADRNPVAWRAALDNLEQT
                     ARRDENVMDALIDAVRAYATIGEITNCLRGVYGEHRALLVV"
     gene            complement(3383..4528)
                     /locus_tag="VFV93_17205"
     CDS             complement(3383..4528)
                     /locus_tag="VFV93_17205"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5167147.1"
                     /translation="MELLFNDEEQTLVEAVREYVARDVAPWAADRDESGSFPTEELLT
                     AAAMGFMGLAVPDDLGGSSLSAASTAIVYEEIARASASVAVILSVHNSLTCNAINQYG
                     TLEIKERLLPRLASGELIGAYCLTEPQAGSDAGAIRTRAVRDGDSWRISGQKAFITSG
                     AHAGVYIVFAVTDPEARTSRRITAFVVERDTPGIEVAPKEKKLGLLASEINGIVFDSV
                     VVPDRQILGEVGGGFGVAMSLLATGRIGIAAQATGIAQAALDAGLAYAKERQQFGQPI
                     AQFQAIQWKLADMATEIDAARLLVRQAALMKDAGRDYIRQASEAKLFASEVASRAAGN
                     AIQIHGGYGYIRDFGVERLYRDAKATELYEGTSEIQRLVIARHLLGQ"
     gene            complement(4570..>5118)
                     /locus_tag="VFV93_17210"
     CDS             complement(4570..>5118)
                     /locus_tag="VFV93_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5167148.1"
                     /translation="VDELAPERTIIASNTSSIPITTLAATTRRPDKVIGMHFFNPVPV
                     MQLVEVVRGLSTGDETVDATIALAKKAGKTPVEVNDFPGFISNRVLMPMINEAIFCLM
                     EGVATRENIDEVMKLGMNHPMGPLALADLIGLDVCLDILEVLQRDLGDPKYRPSPLLR
                     KMVAAGKLGRKTGEGFYDYRQS"
BASE COUNT          805 a         1664 c         1679 g          970 t
ORIGIN      
        1 gtcatcatcg tcgcggccgg gatggatggg gcgttgccgt cggtcgtctc agggctggtg
       61 gacattccgg tcatcggcct accaacgtcc gtcggctatg gcgtggcggc cggtgggcag
      121 gcggcgttga tgacgatgct gtcgacctgt gcgccgggga tggccgtcgt caatatcgac
      181 aatggcgtcg gtgccggcgc gacggcggcg ctgatcgccc gccgcgccca tcagccgcgc
      241 gactgaatcc gcgtcgctgt ctgcctggca agcgcctcgg caacctcaaa cgggttgaca
      301 tctcgatgcc gcagatcggc cagtaccggt tccatgctgg gctcactgag cgtctcgctg
      361 agtcgcgcgc cgagccagtt ctgcgccagc cggaggactt cgcgccgttc acgtcgttcg
      421 cggcggcgct cgccctctcc ggacgcctct aaatgtgcac ggtgtttgtc gatcgtgttc
      481 agcagctcgt cgatatgttc ctcatagacg gcaaccgttc gcacgatcgg tggcagccac
      541 gggccgtgct cgccgagccg cagcatattg cgcaggtcgc ggacgacgtt ctcggcgccc
      601 ttgatgtcgg ccttgttgac gacgaggatg tcggcgatct ccagcacgcc ggccttgatc
      661 gtctggatgc tgtcacccaa ccccggcacc tgaagcagga ggacggtgtc ggccagttcg
      721 gcgacatcga cctcgtcctg accgacgcca accgtctcga cgatgatgat gtcgaatccg
      781 taggcgtcga gcaggtgcgt cacggcgccg accgccatcg agaggccgcc catctcgccg
      841 cgtgtcgcca tcgagcggat gtagacgccg tcgtcgtcgt agttctccag catccgaatc
      901 cggtcgccca gtgtcgcccc gcctgtcagc gagctggacg gatcgatcgc caggacgccg
      961 acggtcttgc cacgcttgcg atactcgcgg atgacggcat tggtcaatgt gctcttgccg
     1021 gcccccggcg ggccggtgat gcccacgaca tacgcattgc cggtgtcgcg atacagttcg
     1081 gccatcgccc gtgtgccggc tggccgctcg ttctcgacgt agctgacgag tcgcgccagc
     1141 gccggccgct cgcgattgcg gaagcgctcg atcaacctgt cggttccagt tgccgtcact
     1201 cgtcaccccg ctgtggcgcg tgttcgcgga tgaagcgaac ggcagattcc agtggcgtgc
     1261 cggggccgaa gacctcggcg acgccaagct ccttaagggc agcgacatcc tcgttcggga
     1321 tcgtgccgcc agcgacgacc agcacgtcgg tgagtcctgc cccttcgagc tcgctgaaga
     1381 tcttcgggaa gagtgcccgg tgcgcgccgg agaggatcga gaggccgatc acgtcgacat
     1441 cctcatcgac ggcggcgcgg gcgatcatct ccggcgtctg gaagaggccg gtgtagatca
     1501 cctccatgcc ggcgtcgcgc agcgcccgcg ccatgacttt ggctccccgg tcgtggccgt
     1561 ccagccccgg tttcgcgatc agcacccgaa tcggcctcgg ccgggcctgc gtttcgatca
     1621 tctcaacgtt cctttcgacc ggcccgcgag ccggcagcct cgcaccctcc gattagacaa
     1681 ccagcagcgc tcgatgctcg ccatacacgc cgcgcaggca gttggtgatc tcaccaatcg
     1741 tcgcgtaggc gcggacggcg tcgatcaacg catccatcac gttctcgtcg cgacgggcag
     1801 tctgttcgag attgtcgagc gccgcccgcc acgcgaccgg gttgcgatcg gcgcgaacgc
     1861 gagccagccg ctccaggtgc cggccctcgc cctgcgggtc catctccaga atgggaatgc
     1921 tcggctcttc ctccgagcgg aaggcattaa cgccgacgac gatcttgcgc tgctcggcca
     1981 gcgcctgctc gtaggcgtag gagctgtcgg agatgcgtcg ctggaagtag ccgccttcaa
     2041 tggccgggac gacgccaccc agcgcgtcga tctcgtcgaa gatcgtttga acccgatctt
     2101 ccatctcgct cgtcatcgtc tcgaccatgt acgagccgcc gagagggtcg acgctgttcg
     2161 cgacgccgct ctcgtgggcg atgatctgct gggtgcgaac ggcgatccgg acggcccagt
     2221 cgctcggcag cgccagggcc tcgtcgagcg agttggtgtg caggctctgg gtgccgccga
     2281 ggacggccgc caatgcctgg atcgtcgtcc ggatgacgtt gttctccggc tcctgagccg
     2341 tcagcgagca gccggcagtc tgggtgtgga agcgcatcca gcaggagcgt tcgttcttcg
     2401 ccccgaaccg ctcgcgcatg acgcggtacc agatgcgacg ggccgcccgg aacttggcga
     2461 tctcctcgaa gaagtcgttg tggcagttga agaagaacga cagccgtggc gcgaaggcgt
     2521 cgatatccag tccccgctca agggccgctt cgacgtaggc gatcccatcg gcgagcgtga
     2581 acgccagctc ttcggtcgcc gtcgcccctg cctcgcggat gtggtagccg gagatcgaga
     2641 tcgagttcca gagcggcatc tggtgggtcg agaactcgat cgtgtcggtc accagcttca
     2701 tcgacggctt cggcgggaag atgtattcgt tctgggcggt gaactccttg aggatgtcgt
     2761 tctggagcgg tccgcgcaac ttggcgcgtg ggacgccctg cttctcggcg gcggcgatgt
     2821 acattgccca gatgacggca gccggtccat tgatcgtcat cgacgtcgtc acggcgtcga
     2881 gcgggatacc gtcgagcagg atctccatgt cggccagcga cgagacggcg acgccgcact
     2941 tgccgaactc gccgtgcgac atcgggtcgt cggtgtcgcg cccgtagagc gtcggcaggt
     3001 cgaaggcgat tgagaggccg gtttcgccgt gctccagcag gtagcggaag cgggcgttgg
     3061 tctcctcggc tgagccgaag ccggcgaact ggcggatcgt ccagagcttg ccgcgataca
     3121 tcgattcgtg gatgcctcgt gtgtacggcg gctggccggg acggcccagg tctttggctt
     3181 catcgaagcc aacccggtcg aggtcatcgc gggtgtagag cggctggacc ggctcgccgg
     3241 agagggttgt gaactcgcgt tcctcaggcg gtgcgccctg atagcgcggg tcgtggaaat
     3301 cggtgctgta acgttgtgac actgccgcct cccttgacgt ttctccgtcg cctcgttgcg
     3361 acgtctgttc agtccgttcc ggtcattgtc ccagcaagtg ccgtgcgatg accagccgtt
     3421 gaatctcgga cgttccttcg tatagttcgg tcgccttggc atcgcgatag agccgttcga
     3481 cgccgaagtc gcggatgtag ccgtagccac cgtggatctg gatcgcgttg ccggccgccc
     3541 ggctggccac ctcgctggcg aacagcttgg cctccgacgc ctggcggatg tagtcgcgac
     3601 cagcgtcctt catcagcgcc gcctggcgga ccaacaagcg ggccgcgtca atctcggtcg
     3661 ccatgtcggc cagcttccac tggatcgcct ggaactgtgc gatcggctgg ccgaactgct
     3721 ggcgctcctt ggcgtaggcg agcccggcat ccagcgcagc ctgcgcgatg cctgtcgcct
     3781 gggcggcgat accgatcctg ccggtcgcca gcagcgacat cgccacgcca aatccgccgc
     3841 caacctcgcc gagaatctga cggtcgggaa cgacaacgct gtcaaagacg atgccgttga
     3901 tctcggacgc cagcaggccg agcttcttct cctttggagc aacctcgatg ccgggagtgt
     3961 cgcgctcgac gacgaaggcc gtgattcggc gcgatgtgcg cgcctcggga tcggtgacgg
     4021 cgaacacgat gtagacgccc gcgtgcgcgc cggaggtgat gaacgccttc tgcccgctga
     4081 tgcgccagct gtcaccgtcg cgaaccgctc gcgtccggat ggctcctgca tctgaaccgg
     4141 cctgtggttc ggtcaggcaa taggcgccga tcaactcccc cgacgcgagc cggggaagca
     4201 atcgttcctt gatctccaga gttccgtact ggttgatggc gttgcaggtc aacgagttgt
     4261 ggacggagag aataacggcg actgacgctg acgctcgcgc gatctcctcg tagacgatgg
     4321 ccgtactggc tgcggagagt gacgagccgc ccaggtcatc cggtacagcc agtcccatga
     4381 agcccatcgc tgcggcggtg aggagctcct cggtcgggaa gctgccactc tcgtcgcggt
     4441 cggcggccca cggcgcgacg tcccgtgcca cgtattcgcg aaccgcctcg accagcgtct
     4501 gctcttcgtc gttgaagaga agctccatct cgcctccgtc gctcaggttg tgcgttgccg
     4561 gtcagccgtt tacgactgcc ggtagtcgta gaagccctcg cccgtcttgc ggcccagctt
     4621 gccggccgcg accatcttgc gtagcagtgg tgaaggccgg tacttcgggt cgcccagatc
     4681 acgttgcagg acttcgagga tgtcgaggca gacatccagc ccgatcaggt ccgccagcgc
     4741 cagcggcccc atcgggtgat tcatgcccag cttcatgacc tcgtcaatgt tctcgcgggt
     4801 ggcgacgcct tccatcagac agaagatcgc ctcgttaatc atcggcatca ggacgcggtt
     4861 ggagatgaag ccggggaagt cattgacctc gaccggcgtc ttgccggctt tcttcgccag
     4921 cgcgattgtc gcgtcaaccg tctcgtcgcc ggtcgagagt ccacggacga cctcgacaag
     4981 ctgcatgacg ggaaccgggt tgaagaagtg catgccgatc actttgtccg gccggcgggt
     5041 cgtcgcggcg agcgtagtga tcggaatcga tgaggtattt gaggcgatga tcgttcgctc
     5101 aggcgccagc tcgtcaac
//
LOCUS       DASUGJ010000762         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_960165, whole genome shotgun sequence.
ACCESSION   DASUGJ010000762 DASUGJ010000000
VERSION     DASUGJ010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960165"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFV93_17215"
     CDS             complement(<1..220)
                     /locus_tag="VFV93_17215"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX5167149.1"
                     /translation="MIGILLDEHVSGPVIGRALADDGRDVRAADSDETLRESEDRILF
                     AIARAERRLLVTNNTKDFAPMIRKLADRG"
     gene            complement(217..642)
                     /locus_tag="VFV93_17220"
     CDS             complement(217..642)
                     /locus_tag="VFV93_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167150.1"
                     /translation="MATVTTRQLTIQIPEEAYAWLEQEARDAGQPIEVFAGLLVEEAR
                     RVEQFSGIFFRRGGGDGRRAVIIGGMDVWEMIMLYQAHGREGMLEAFDSIQEFEIDQA
                     LAYYEAHPEDIDERLWENSQPMEYWLEKYPELRAQVREI"
     gene            797..2050
                     /locus_tag="VFV93_17225"
     CDS             797..2050
                     /locus_tag="VFV93_17225"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX5167151.1"
                     /translation="MTATTTVPLNPTQLRTDFPILAQTHNGHPLAYLDSAASSQKPQV
                     VIDAVRNFYEQDNANVHRGVYALSERATDAYEQARRKVARFFNAPHARQIIFTRNTTE
                     ALNLVANSWGRWALRPGDTVLISELEHHSNIVPWQLVCQATGATLDYIAITDDGRLQL
                     DDLDRHLATGRVRMVSLCHVSNVTGTINPVEEIIARAHAAGVLVTLDGAQAAPHLPVD
                     LTALDVDFYACSGHKMCAPMGAGVLYGRRELLEAMPPFLGGGSMIRQVGLQSSTWADL
                     PEKFEAGTPNVADAIGLGVACDYLSAIGLDAIHAHEQALTRYALDRLSDIPGLTLFGP
                     PAEHRCGVAAFTLDGVHPHDIGSILDETGVCVRAGHHCTQPLHARFAIDASARASFYL
                     YNTQEDIDRLIDGLARVQSIFGIGG"
BASE COUNT          406 a          746 c          566 g          417 t
ORIGIN      
        1 cgcctcgatc cgcgagttta cgaatcattg gtgcaaagtc cttggtattg tttgttacga
       61 gtagccttcg ttcagcgcgc gcgatagcga acaggatgcg gtcttcgctt tctctcagcg
      121 tctcgtccga gtcggcggca cgaacgtcac ggccgtcatc ggccaacgcg cgaccgatga
      181 cgggtccaga gacatgctcg tcaagcagga tgccgatcaa atttcgcgaa cctgtgcgcg
      241 aagttctggg tacttctcga gccagtattc catcggctgg ctgttttccc aaagtcgttc
      301 gtcgatgtct tcaggatgcg cctcatagta ggcgagggct tgatcgatct cgaactcctg
      361 aatactatcg aacgcttcga gcatgccctc tcgaccgtga gcctggtaca gcatgatcat
      421 ttcccagacg tccatcccgc cgatgatcac ggcccgccga ccgtctccgc cgcctctgcg
      481 aaagaagatg ccactgaatt gctcgactcg ccgtgcttcc tcgacaagga gacctgcgaa
      541 cacttcgata ggttggcccg cgtcccgcgc ctcttgctcc agccatgcat aggcttcttc
      601 agggatttga atcgttagct gtcttgtcgt cacagtagcc atgtggtgct ccaggaggta
      661 gtacgtcagc gccgataaca gattttactc gtacgcgacg tggctaagtt caaagccgtt
      721 tgtaccattc aaggttcgca tcactccaga ggtcggcaga agatgcacga ttccagcgaa
      781 cgaaggattg acaacgatga ctgctacaac gaccgtaccg ctcaacccca cacaactccg
      841 caccgacttc ccgatcctcg cgcagactca caacggccat ccgctcgcct atctggacag
      901 cgccgcgtca tcccagaagc cacaggtcgt catcgacgcc gtccgcaact tctacgagca
      961 ggacaacgcc aatgtccacc gcggcgtcta cgcgctctcc gagcgagcca ccgatgccta
     1021 cgagcaggcc cggcggaaag tcgcccgttt cttcaacgcc ccccacgcgc gtcagatcat
     1081 cttcacccgc aacacgactg aggcgctcaa cctcgttgcc aacagctggg gccgctgggc
     1141 actccgtcct ggtgacaccg tcctgatcag cgaacttgag catcactcca acatcgttcc
     1201 ctggcagctc gtctgccagg cgaccggcgc gacgctcgat tacatcgcca tcaccgacga
     1261 cggacgcctt cagttggacg accttgaccg ccacctcgcg accggccggg tccggatggt
     1321 cagcctctgt cacgtctcca atgtgaccgg cacgatcaat cccgtcgagg agatcatcgc
     1381 ccgtgctcac gctgccggcg tgctcgtcac gctcgacggc gcccaggccg cgccgcacct
     1441 cccggttgat ttgaccgcgc tcgacgtcga cttctacgcc tgctccggtc acaagatgtg
     1501 cgccccgatg ggggccggtg tcctctacgg ccgtcgcgaa ctgctcgaag cgatgccgcc
     1561 gtttctcggt gggggatcga tgatccgcca ggtcggcctc cagtcatcga cctgggccga
     1621 cctgcccgag aagttcgaag ccggcacgcc caatgtcgcc gacgcgatcg gcctcggcgt
     1681 cgcctgcgat tacctcagtg ccatcggcct cgatgccatc cacgctcacg agcaggcact
     1741 gacccgctat gccctcgacc ggttgtcaga tatccccgga ctgaccctct ttggccctcc
     1801 ggccgagcat cgctgtggcg tcgccgcctt cacgctcgac ggcgtccacc cgcacgacat
     1861 cggcagcatt ctcgatgaaa cgggcgtctg cgttcgggcc ggccaccact gcacccagcc
     1921 gctacatgcc cgcttcgcga tcgatgcctc agcccgcgcc agcttctatc tctacaatac
     1981 ccaggaagat atcgatcgcc tcatcgatgg acttgcgcgg gtccagtcca tctttggcat
     2041 cggcggctga cacaatgacc gacgcccagg ccgatatgca acaatccacg cagccgccag
     2101 caccatgctg aacgtcgcac gaacggaaac caggc
//
LOCUS       DASUGJ010000763         5453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1236741, whole genome shotgun sequence.
ACCESSION   DASUGJ010000763 DASUGJ010000000
VERSION     DASUGJ010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236741"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..528
                     /locus_tag="VFV93_17230"
     CDS             46..528
                     /locus_tag="VFV93_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010174760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5167152.1"
                     /translation="MSDTTAERVVTTVDELKELIGQEIGVSDWLEITQERVNQFADAT
                     GDHQYIHVDPERAAKTFFGGTIAHGYLTLSLIPALSGTRQGVKINLGGKMGVNYGLNK
                     VRFTAPVPVGKRIRSRTTLLAVEEIGDRAVQTTNQVIIEVEGGDKPACIAETLGRTYF
                     "
     gene            537..1031
                     /locus_tag="VFV93_17235"
     CDS             537..1031
                     /locus_tag="VFV93_17235"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5167153.1"
                     /translation="MDLIDRLVGHDLWTTNEILRRCQELTDAQIDQEFDIDHRTVRET
                     LIHMIANMETWAALMEPPPASVTQRIWGESVDSLTERHRAAGEKFRSVARRIAHEGRL
                     EERFLDFLDDPPREKPNGAGIAHIITHDMHHRAQLLYMLDKLGLQDLPEGDLMGWEMR
                     YRFQ"
     gene            1086..3281
                     /locus_tag="VFV93_17240"
     CDS             1086..3281
                     /locus_tag="VFV93_17240"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX5167154.1"
                     /translation="MATTERRSDVDEQQAFTRAMDALFHPKRIAIVGATPRLGFANGI
                     HRLILQGGYEGEVYGVNPNYQEVLGSPCVPTLEDIPGGADKAVVVVPSQHVMNVLGQA
                     ERSGVKAVNIITSGFGEQADDEAHERQDEIRRFAQRTGIRVVGPNCLGIISTPAKMIA
                     KSGPYTTVKRGPIGIVFQSGLMAYSVVLPPQDRGFGYSYVVTTGNEADLDATDFMRYF
                     IEDDETKVVGCFIEQFRDADKLIAVAERALELRKPLVVLKIGRSEGGQRAARAHTGSL
                     VGSDDVIDTVMRQHGIIRVYNMDEMIETLAVFHSKRLPKSTGVGTIFVSGGAGGLISD
                     LSQDLGINLPELSPATATRLKGIIPAYGTVGNPLDTTGQVAQQPEIMQGVLESMAEDP
                     NVATVVYGQAYPSMLDLASPVGEVMRVMPDKYPDKVFLIMSLVHGKMQEGYRAGMAPQ
                     EPVNEWNGTPFLQGTENGLRAIRSLNRYAQFLRDREAEGVQASATDTAIAEQAREIVH
                     AAGGRPLVEREAKQLLALYDIPVTEERLVTNADDAVSAANEIGYPVVLKIESPDIAHK
                     TEAGGVLLNLAGAEAVRAGFQQIIDSARAYNAAAEISGVLVQEMVQGGRELILGMTQD
                     PTFGPAVAVGLGGIFVEILKDVQLGVPPLTGTDARGMLDRLRGKAILEGARGQQPADI
                     DAVTGILSRFSRLCLDLRDEVSEIDINPLLVFEQGKGAKVVDCLIVPSR"
     gene            3400..4002
                     /locus_tag="VFV93_17245"
     CDS             3400..4002
                     /locus_tag="VFV93_17245"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5167155.1"
                     /translation="MTLGTRVGVFADVHGSTRTLERALEVCRKEGVETIALLGDLFDR
                     HEQADPCALVLAGWDVVGVYGNHEREVAIEAALGVYPLLEETVRLLSALEEEVVIGDV
                     RLTHEVEHWGHIDPIARLFGRNQHANGHHGSVTERLTFTGHTHFRQARDERGTLDIGR
                     GALSLDSSRRYLINPGALLIGQYAIWDREEDIVFFRNVEH"
     gene            4175..5221
                     /locus_tag="VFV93_17250"
     CDS             4175..5221
                     /locus_tag="VFV93_17250"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5167156.1"
                     /translation="MGKLDGKVALVTGAGRGIGRGIALLMAQEGAAVVVNDLGVSLDG
                     EGQDTGPAASVVREIEAMGGQGMANTDSITDYDAVGAMVEGAIDRFGKLDIVVNVAGI
                     LRDRMLFNMNDAEWDAVIDVHLRGTFNTCRHASAHFRERREGGRIVNFSSSSAWGSPG
                     QPNYAAAKYGILGLTATLANSLNRYGVTANAILPYAATRMIDSTPRAEKVREETGKLP
                     SELAIGSEGDPANVAPMVCYLATDDASMINGHFFGVGGYTVSLYSHWEIAGVLAAERR
                     WTVEELIEQFPATIGKRVAVPEPVQVTGSDRPLKGSAAMQADEASWTEISDGIKLWTR
                     KIYDEAGSTLVRDA"
     gene            5317..>5453
                     /locus_tag="VFV93_17255"
     CDS             5317..>5453
                     /locus_tag="VFV93_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5167157.1"
                     /translation="MSILEGKVALVTGAGRGIGRGIAHLLAEHGAAVVVNDLGATLDG
                     EG"
BASE COUNT         1023 a         1769 c         1813 g          848 t
ORIGIN      
        1 agataccggt cctcgaacag gaatcggaaa ggcgaagggg caaccatgag cgacacgact
       61 gccgagcgag ttgtcacgac cgtagacgag ctcaaggaac tgatcggcca ggagatcggc
      121 gtcagcgact ggctggagat cacccaggag cgggtcaacc agttcgccga cgcgaccggc
      181 gaccaccagt acatccacgt cgacccggag cgggcggcga agaccttctt cggtgggacg
      241 atcgcccacg gctatctgac gctctcgctg atcccggccc tgtccggcac gcgtcagggc
      301 gtcaagatca acctgggtgg caagatgggt gtcaactacg ggctgaataa ggtccgcttc
      361 acggcaccgg tcccggtcgg caagcgcatt cgctcgcgga cgacgctgct ggctgtcgag
      421 gagatcggcg accgggccgt ccagacgacc aaccaggtca tcatcgaggt cgagggcggc
      481 gacaagccgg cctgtatcgc cgagacactc ggccgcacct acttctaggg cggccgatgg
      541 atctgatcga ccggctcgtg ggccacgacc tctggacgac caacgagata ttgcgccgtt
      601 gtcaggaact gacggacgcg cagatcgacc aggagttcga catcgaccat cggacagttc
      661 gggagacgct gatccacatg atcgcgaaca tggagacctg ggccgcgctg atggagccgc
      721 cgcctgcatc ggtgacacag cggatctggg gggagtcggt cgattcgctg actgaacggc
      781 accgggccgc aggtgagaag tttcgcagcg tggccaggcg gatcgctcat gaagggcgtc
      841 ttgaagagcg attcctcgac ttcctggacg atccgccacg tgagaagccc aacggagccg
      901 ggatcgcgca catcatcacc cacgatatgc accatcgcgc gcaactgctc tacatgctcg
      961 acaagctcgg cctacaggat ctgccggaag gcgacctgat gggctgggag atgagatatc
     1021 ggtttcagtg acgaacgttc cgctcgggcg aggggcgagt gaccgaggag cgagggaggg
     1081 gttcggtggc gacgacagag cgacgatcag acgttgacga acaacaggcg tttacccgcg
     1141 cgatggacgc gctctttcat cccaaacgga tcgcgatcgt cggggcgacg ccccggctcg
     1201 gcttcgccaa cggcatccac cgcctgatcc tgcaaggcgg ctacgagggc gaggtctacg
     1261 gcgtcaaccc caactaccag gaagtgctcg gctcgccctg cgtcccgacg ctggaagaca
     1321 ttcccggcgg ggccgacaag gcagtcgtcg tcgtcccgag ccagcacgtg atgaacgtcc
     1381 tcggccaggc cgagcggtcg ggcgtcaagg ccgtcaacat catcacctcc ggcttcggcg
     1441 agcaggccga tgacgaggcc catgagcggc aggacgagat ccgccgcttc gcccagcgaa
     1501 cggggattcg cgtcgttggg ccgaactgcc tcggcatcat cagcacgccg gccaagatga
     1561 tcgccaagtc ggggccatac acgaccgtca agcgcggccc gatcgggatc gtcttccaga
     1621 gcggactgat ggcctactcg gtcgtcctgc caccgcagga ccgtggcttc ggctactcgt
     1681 atgtcgtcac gaccggcaac gaggccgacc tcgacgcgac cgacttcatg cgctacttca
     1741 tcgaggacga cgagaccaag gtcgtcggct gcttcatcga gcagttccgc gacgccgaca
     1801 agctgatcgc tgtcgccgag cgggcgctgg agctgcgcaa gccgctggtc gtcctcaaga
     1861 tcggccgctc cgagggcggc cagcgggcgg ccagagcgca caccggctcg ctggtcggct
     1921 ccgacgacgt gatcgacacc gtcatgcgcc agcacggcat catccgcgtc tacaacatgg
     1981 acgagatgat cgagacgctg gccgtcttcc acagcaagcg gctgccgaag tccaccggtg
     2041 tcgggacgat cttcgtctct ggcggcgccg gcggcctgat ctccgacctg agccaggacc
     2101 tcggcatcaa cctgccggag ctgtcgccag cgaccgcgac gcggctgaaa ggcatcatcc
     2161 cggcctatgg gacagtcggc aacccgctcg acacgaccgg ccaggtcgcc cagcagccgg
     2221 agatcatgca gggcgtgctt gagtcgatgg ccgaagaccc aaacgtcgcc actgtcgtct
     2281 acggtcaggc gtatccgtcg atgctcgacc ttgcctcgcc ggttggcgaa gtcatgcgcg
     2341 tcatgccgga caagtacccc gacaaggtct tcctgatcat gtcgctcgtc cacggcaaga
     2401 tgcaggaggg ctaccgggcc gggatggcgc cacaagagcc ggtcaacgag tggaatggca
     2461 ccccgttcct gcaagggacc gagaacggct tgcgcgcgat tcgctcgctc aaccgctacg
     2521 cgcagttcct gcgcgaccgc gaggcggagg gcgtgcaagc gtccgccact gatacggcca
     2581 tcgccgagca ggcgcgcgag atcgtccacg ccgctggtgg ccggccgctg gtcgagcgtg
     2641 aggcaaagca actgctggcg ctctacgaca ttccggttac cgaggagcgg ctggtgacga
     2701 acgccgacga cgccgtctca gccgccaacg agatcggtta cccggttgtt ctcaagatcg
     2761 agtctcccga catcgcccac aagaccgaag ctggcggtgt cctgctcaac ctggccggcg
     2821 ctgaagccgt ccgggccggc ttccagcaga tcatcgacag tgcccgcgcc tacaacgccg
     2881 ccgccgagat cagcggcgtt ctcgttcagg agatggtcca gggtggccga gaactcatcc
     2941 tcggcatgac gcaggacccg accttcggcc cggccgttgc ggtcggcctc ggcggcatct
     3001 tcgtcgagat cctcaaggac gtccaacttg gcgtcccgcc gctgaccggc accgacgcgc
     3061 gcggcatgct cgaccggctg cgcggcaagg cgatcctcga aggcgcacgg ggccagcagc
     3121 cggccgacat cgacgccgtc accggcatcc tgagccgctt ctcgcgtctc tgcctcgacc
     3181 tgcgcgacga ggtttccgag atcgacatca acccgctgct cgtcttcgag cagggcaagg
     3241 gtgcgaaggt cgttgactgt ctgattgtgc cgagtcgttg atcgggacgc ggaaaatgac
     3301 acgagggagt atgacaacgc acggcagaac cggattcaaa cgttcgaccc gtaatgcgcc
     3361 cacgcgtccc gtgtcccgcg tcccgcgtcc cgtccgccca tgacattggg gactcgcgtc
     3421 ggcgtcttcg ccgacgtaca tggatcgacg cgaacgctgg agcgggcgtt ggaagtctgc
     3481 cgcaaggagg gcgtcgagac gatcgcgctc ctcggcgatc tgttcgaccg gcacgagcag
     3541 gccgacccgt gcgcgctcgt cctggccggc tgggatgtcg tcggcgtcta cggcaaccac
     3601 gaacgggagg ttgcgatcga ggcggcactc ggtgtctacc cgctgctgga ggagactgtg
     3661 cggttgttga gcgcgctgga ggaggaagtc gtcatcggcg acgtccggct gacgcatgag
     3721 gtcgagcact ggggccacat cgatccaatt gcgcggctct tcggccgcaa ccagcacgcc
     3781 aatggccacc acggctccgt caccgagcgc ctcaccttca ccggccatac ccacttccgc
     3841 caggcccgcg acgaacgcgg caccctcgac atcggccgcg gcgcgctctc gctcgattcc
     3901 tcaagacggt atctgatcaa tccgggggcg ctgctgatcg gacagtacgc gatctgggac
     3961 cgggaggagg acatcgtgtt cttcaggaac gtagagcatt gaccgcgcgt cagaagtagg
     4021 ggccggtctt gtgccggccc tgttcctcca cccgacacgc tcgggaaccg aagcagggcc
     4081 ggcacacgac cggcccctac ttctgacgcg gatttgcgtg gattcggtta cagtgagcag
     4141 cacatgaaac caacggcatg gactgggagg gtacatgggc aaactggatg gaaaggtcgc
     4201 tctcgtaaca ggggcgggac gcgggatcgg gcgcgggatt gcgctgttga tggcgcagga
     4261 gggggcggcg gtcgtcgtca acgacctggg tgtctcgctg gacggcgagg gacaggatac
     4321 cggaccggcg gcatccgtcg tccgtgagat cgaggcgatg ggcggccagg ggatggccaa
     4381 caccgactcg atcaccgact acgacgcggt cggcgcgatg gtcgagggcg cgatcgacag
     4441 gttcggcaag ctcgacatcg tcgtcaacgt cgccggcatc ctgcgcgacc ggatgctgtt
     4501 caacatgaac gacgccgagt gggacgccgt tatcgatgtc catctgcgcg ggacgttcaa
     4561 cacctgccgg catgcctcag cccacttccg cgagcgacga gagggcggac ggatcgtcaa
     4621 cttctcgtcg tcttcggcct ggggcagtcc cggccagccc aactacgccg ccgccaagta
     4681 cggcatcctc ggcctgacgg cgaccctggc aaactcgctc aaccgctacg gcgtgaccgc
     4741 caatgcgatc ctgccctacg ccgcgacgcg gatgatcgac tcgacgccgc gagccgaaaa
     4801 ggttcgcgag gagaccggca agctgccgag tgagctggcg atcggctccg agggcgatcc
     4861 ggccaacgtc gcgccgatgg tctgctatct ggcaaccgac gacgcctcga tgatcaacgg
     4921 ccacttcttc ggcgtcggcg gttacaccgt ctcgctctac tcccactggg agatcgccgg
     4981 cgtcctggct gccgaacggc gctggaccgt cgaggaactg atcgagcagt tcccggccac
     5041 gatcggcaag cgggtcgccg tccctgagcc ggtccaggtg accggcagcg accgcccgct
     5101 caagggatcc gccgcgatgc aggccgacga ggcaagctgg accgagatca gcgacggcat
     5161 caagctctgg acgcgcaaga tctacgacga ggcggggtcg acgcttgttc gtgacgcgtg
     5221 atgcgtgacg cgtgacgcga caatgtgaga cttcgaagca gtccatcccg ttcgacgcgt
     5281 cacgcgtcac gcgtcacgcg ttacgaaagg aacaagatga gcattctgga aggtaaagtc
     5341 gcgctggtga ccggcgcggg acgcgggatc ggcaggggca tcgcgcatct gctggcggaa
     5401 catggggcgg cggtcgtcgt caacgacctg ggggccacac tggacggcga ggg
//
LOCUS       DASUGJ010000764         4993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1794260, whole genome shotgun sequence.
ACCESSION   DASUGJ010000764 DASUGJ010000000
VERSION     DASUGJ010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794260"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFV93_17260"
     CDS             <1..181
                     /locus_tag="VFV93_17260"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5167158.1"
                     /translation="TLPEYRRQGIGAAMTRRCIDDGWRSGCTVSALQSSSSGFPVYQR
                     MGYRHVFDIQGWRFA"
     gene            complement(275..634)
                     /locus_tag="VFV93_17265"
     CDS             complement(275..634)
                     /locus_tag="VFV93_17265"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX5167159.1"
                     /translation="MSTRRLRMLALIAVAALGALLLAGCGDSSSASEPVETNAVNLPP
                     SYKFEPKVIQVTAGTTVTWTNNDHFTHSVEFDGQPDRQLKPGESVEVTFDTPGTYDYK
                     CTLHPHDMSGTVIVVSN"
     gene            complement(631..939)
                     /locus_tag="VFV93_17270"
     CDS             complement(631..939)
                     /locus_tag="VFV93_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167160.1"
                     /translation="MPYGTFVNELPVAFFMIVHLSAFLIGAFFAQRALTLGKTFAGWG
                     FALYAAAEIVYMTYHLDWTIFLFAHTVAEVLNLVAYALIFASVVEWAGGSLRHQVSES
                     "
     gene            complement(969..1610)
                     /locus_tag="VFV93_17275"
     CDS             complement(969..1610)
                     /locus_tag="VFV93_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167161.1"
                     /translation="MSRRSLLAAAAGAGTGAVAVAALGPILTTEAASRDDRERLRTTM
                     RKLWEDHVTWTRMVIVSAIAGSPDLNAATQRLLSNQDDIGDAIVPYLGFDAGAGLAEL
                     LREHIMVAATILGAATAGDNGTFNAALTDWYANGDKIAVFLHLANPRMWPLGQMKAMM
                     REHLDLTLAEATARLTADWEGDVVAYDRVHDAILKMSDMITDGLLKIPGHGGH"
     gene            complement(1702..1929)
                     /locus_tag="VFV93_17280"
     CDS             complement(1702..1929)
                     /locus_tag="VFV93_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167162.1"
                     /translation="MRWPARLFWIGLLIVAAGAAGDILHHALPAHLSSNLNLFLGADG
                     GRAHLLTLVGMVLTMLGLGSFATYRHQPPAD"
     gene            2148..2783
                     /locus_tag="VFV93_17285"
     CDS             2148..2783
                     /locus_tag="VFV93_17285"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX5167163.1"
                     /translation="MSMQRWDQRFLATTRGRVVMLLRRASRTVNDLAGELELTDNAVR
                     SHLATLERDGLVRQEGVRREGHKPSYAYGLTPEAETLFPKAYGPVLGSVVEVVCNRLG
                     PDETRAILEEVGRRLATTLDQDAGIDERVDRAAATIGDLGGLAEVEQAGDHYLLRGFA
                     CPLAEAVRSAPATCQIAESLLAQATGLDVRECCDRSGTPRCRFELRTPPSS"
     gene            2952..3473
                     /locus_tag="VFV93_17290"
     CDS             2952..3473
                     /locus_tag="VFV93_17290"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX5167164.1"
                     /translation="MAHHVAIAYPDHARATEVIAALKRLEAEGVVELKKAVAIIHERE
                     RQLGAGSVIAQSIVGALAGSVVGVLLGLAFSDLWLGTLIGAVVGALSGLMIAFARSEE
                     PHSYGFGSFGQQAAGRLPEGGAAVLMLVRKNDPEKAIATLKEYGGRVLHTTLPADVDS
                     QLRAAVSNLAPAA"
     gene            4048..>4993
                     /gene="argF"
                     /locus_tag="VFV93_17295"
     CDS             4048..>4993
                     /gene="argF"
                     /locus_tag="VFV93_17295"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5167165.1"
                     /translation="MKDLLKTSDLTIEDMHLLLDLAAEFKANPMKEYGLLTGKTVVLY
                     FAKPSTRTRLSFETAVARLGGVPSTVGPQELQIGRGESLEDTAKVVSRYAEAFVIRTY
                     ADDDVQLVAEAATIPVINALTDGHHPCQCLADILTLKEAWGTFSGKQLAYVGSDNNVL
                     TSLMQASAMAGLHLTIATPPGYEPDETLFAKSWGIAKKMGGSVNIMHEPADAVKGADA
                     VYTDAWLSMGDSELTRAERFAALTPYRVTPQMMSQANSDAVFMHCLPAHRGEEVTAEV
                     IDGPQSIVFDQAENRLHTGQAVLYALITGKLTGTSTTIP"
BASE COUNT          836 a         1614 c         1639 g          904 t
ORIGIN      
        1 gacgctgcct gagtaccgtc gccagggcat cggggccgca atgacccggc gctgcatcga
       61 tgatggctgg cgcagcggct gcaccgtctc ggcgctgcaa tcgagcagca gcggctttcc
      121 ggtctatcaa cggatgggct atcggcatgt gttcgacatc cagggttgga gatttgcctg
      181 agcggaccaa ctgaccaacc agggatggta cagacagcgt gacgcgggcc ggtcccctgg
      241 ccggcccgcg gcgatggtcg acgtcggtga gtgactagtt cgatacgacg atgacggttc
      301 cgctcatatc gtgcggatgc agcgtgcact tgtagtcgta ggttcccggt gtgtcgaagg
      361 tgacctcgac gctctcacca ggcttgagct ggcgatccgg ttggccgtca aactcgacgc
      421 tgtgggtgaa gtggtcgttg ttcgtccagg tcacggtcgt gccggccgtc acctgaatga
      481 ccttcggctc gaacttgtag ctgggcggca ggttgaccgc attggtctcg actggctcgc
      541 tggcgctgct actgtcgccg cagcccgcca gcagtagcgc ccccagcgcc gcgacagcga
      601 tcaacgcaag cattcgcaac cgtcgcgtgc tcatgattcc gacacctgat ggcgcaggct
      661 gccgccggcc cactcgacga cgctggcgaa gatcagcgcg taggcgacca gattcagcac
      721 ctcggcgacg gtatgggcga acaggaagat cgtccagtcc aggtgatagg tcatgtagac
      781 gatctcggcg gcggcgtaga gggcgaatcc ccagccggcg aaggtcttgc ccagcgtcaa
      841 cgcgcgctgg gcaaagaagg caccgatcag gaaggcgctc agatgaacga tcatgaagaa
      901 ggcgaccggt agctcgttaa cgaatgttcc gtatggcatg tctcatcctc tcatctgtgc
      961 gaggcggctc aatgaccgcc gtggccgggg atcttcagca gaccgtcggt gatcatgtcg
     1021 gacatcttga gaattgcgtc gtggacccgg tcgtaggcca cgacatcgcc ttcccagtcc
     1081 gccgtgagcc gggcggttgc ttcggcaagt gtgagatcga ggtgttcgcg catcatcgcc
     1141 ttcatctgcc ccagcggcca catgcgtggg ttcgccaggt gcaggaagac ggcgatcttg
     1201 tcgccgttgg cgtaccagtc ggtcagcgcc gcgttgaacg tgccgttgtc gccggctgtg
     1261 gcggcgccga ggatcgtcgc cgcgaccatg atgtgctcgc gcagcagctc agcgaggcca
     1321 gcgccggcgt cgaagccgag gtacggcacg atcgcgtcgc cgatgtcatc ctgattcgag
     1381 agcagacgct gggtggcggc gttgaggtcc ggcgatccgg cgatcgcgct gacgatcacc
     1441 atccgcgtcc aggtgacgtg gtcctcccag agcttgcgca tcgttgtccg cagccgctcg
     1501 cggtcgtcgc ggctggccgc ttcggtcgtc aggatcggcc cgagggcggc gacggcgacc
     1561 gccccggtac cggcaccagc cgcggccgcc agcagggatc ggcggctcaa cggcctgcgt
     1621 gtcaacggtg gtcggccgga agactggtcc atggttcttc ctttcagcgt tgatgggtac
     1681 tcacgttggt ccgacgcggc gtcaatccgc cggcggctgg tggcgatagg tggcgaagct
     1741 gcccaggccg agcatggtga ggaccatccc caccagtgtc agcagatggg ctcgcccgcc
     1801 atccgctccg agaaacaggt tgagattgct gctcaggtgg gcaggcagcg catgatggag
     1861 gatgtcgccg gcagcgccgg cggcgacaat cagcagcccg atccagaaga gccgcgccgg
     1921 ccatcgcatc ggccagcccc ctcggtcctg actacccacc tggtcacccc tttctggtcg
     1981 tggacccggc tgaaacttcc aggcccgctc gattgatcgt gttgtgacca gcataggagt
     2041 ttcagactat aagtcaagat atgatttgac tttcttgaca aattacgaca ttcttcgtat
     2101 accgagagcg cgcgaccgag attcggccgg aacggagggt gtcgccaatg tcgatgcagc
     2161 gttgggacca gcgctttctt gccaccaccc gtggtcgggt cgtcatgctg ctgcggcgcg
     2221 ccagccggac cgtcaacgat ctcgccgggg agctggagct gaccgacaac gccgtccggt
     2281 cccacctcgc caccctcgaa cgagatggcc tggtccgcca ggagggcgtt cgccgcgagg
     2341 gacacaagcc gtcgtacgcc tacgggctga cgccggaagc cgagacgctg ttcccgaagg
     2401 cgtacgggcc ggtgctcggc tcggttgtcg aagtcgtctg caaccgcctc ggcccggacg
     2461 agacgcgcgc gatcctggag gaggtcggcc ggcgactggc gacgacgctt gatcaggatg
     2521 ccggcatcga cgagcgcgtc gatcgggcgg ccgcgacgat cggcgacctg ggcggcctgg
     2581 ccgaggtcga gcaggcaggc gaccactacc tgctgcgcgg cttcgcctgt ccgctggcgg
     2641 aggcggtccg cagcgcgccg gccacctgcc agatcgccga gtcactgctg gcccaggcga
     2701 ccggcctgga tgtccgcgag tgctgcgatc gctccggcac cccccgctgc cgcttcgagc
     2761 tgcgcacccc accgtcgtcc tgaggccgcg ttgtttcttt cctgacgatg gactatggtt
     2821 gagggagatg acgcaacatc ggtgcgctca tcgatattgg ccacgacgga gttcatgtcg
     2881 tggcagaacg atgatcgacg gtcggcggcc cgattgggta gccggccgtt cgtgcagcaa
     2941 ggaggagtgt cgtggcccat cacgttgcga ttgcctaccc ggaccatgcc cgggcgaccg
     3001 aggtaatcgc cgccctcaag cggctcgaag ctgagggtgt tgtcgagttg aagaaggcag
     3061 tggcgattat ccacgagcgc gagcgccagc ttggcgccgg ctcggtcatc gcccagtcga
     3121 tcgtcggtgc gctggccggg tccgtcgtcg gcgtcctgct cggcctggcc ttcagtgatc
     3181 tgtggctggg aactttgatt ggcgccgtcg tcggcgcgct ctccggcctg atgatcgcct
     3241 tcgcccgctc cgaggagccg cactcgtacg gtttcggctc ctttggccag caggccgccg
     3301 gccgcctgcc cgaaggcggc gcggccgtcc tgatgctggt ccgcaagaac gacccggaga
     3361 aggcgatcgc caccctcaag gagtacggcg gccgcgtcct ccatacgaca ctgccggccg
     3421 atgtcgattc gcagttgcgg gctgccgtca gcaacctcgc gccggctgct tagccaattc
     3481 cagtagccca atccggtacg ggcgatatcg caatatcgcc ctgctctccc ccccgatcgt
     3541 tgtggtagga acccagggcg atattgcgat atcacccgta ccggattgat tcattgaggg
     3601 ttgattgtcc agtacggtac tttcggaatg cttcaaaata ctggacttcc ggttacgggc
     3661 gtgctagagt gtccgaatcg cgcccgcgat gggtgcattc attggagtgg tcgccgctat
     3721 ggcagcacag ttcggacagt tgaggcagag gtcgatcagg cgcaggtaat cctgccgtcg
     3781 cgttcgtccg ttgtgtctcg attcgtctgg agtgttcgat gaccgctcaa gctctttccg
     3841 ccgacaacct gttcacgatc gccactggtg ctggattcct gcaccacggt tcctccaccg
     3901 cgctcaggcg cgtcaggcgc tgcgcctgac gacgctgctg ccgatccccg tccgtgatgg
     3961 cagcgactcg acgtcaggcg ttccggcacc tgtcgtttgt gttgcctgac catctgcgca
     4021 gactgcgcac ccgtggagga aagacccgtg aaggacctgc tgaagacatc cgacctgacg
     4081 attgaggaca tgcacctgct gctcgacctg gcagctgagt tcaaggccaa cccgatgaag
     4141 gagtatggcc tgctgaccgg caagacggtc gtcctctact ttgccaagcc gagcacccgg
     4201 acgcgcctgt cgttcgagac ggctgttgcg cgcctcggcg gcgttccgtc gacggtcggc
     4261 ccgcaggagc tccagatcgg gcgcggcgag tcgctggagg acaccgccaa ggtcgtctcc
     4321 cgctacgcgg aagcgttcgt gatccgcacc tacgccgacg acgatgttca gctggtcgcc
     4381 gaggcggcca cgatcccggt catcaacgcg ctgaccgacg gccaccaccc gtgtcagtgc
     4441 ctggccgaca tcctgacgct caaggaagcc tgggggacgt tctccggcaa gcagctcgcc
     4501 tatgtcggca gcgacaacaa cgtgctgacg tcgctgatgc aggccagcgc gatggccggc
     4561 ctgcacctga cgatcgcgac gcctcccggc tacgaaccgg acgagacgct gttcgccaag
     4621 tcgtggggca ttgccaagaa gatgggcggc tcggtcaaca tcatgcatga gccggctgac
     4681 gccgtgaagg gcgccgatgc cgtctacacc gacgcctggc tctcgatggg tgactccgag
     4741 ctgactcgcg ccgagcgctt cgccgcgctg acgccatacc gcgtcacgcc gcagatgatg
     4801 tcccaggcca actccgacgc cgtcttcatg cactgcctgc cagcccaccg tggcgaggaa
     4861 gtgacggcgg aagtcatcga cggcccgcag tcgatcgtct tcgaccaggc cgagaaccgg
     4921 ctccacaccg gccaggccgt cctctacgcc ctcatcaccg gcaagctgac cggcaccagc
     4981 acgacgatcc cgg
//
LOCUS       DASUGJ010000765         5239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1098813, whole genome shotgun sequence.
ACCESSION   DASUGJ010000765 DASUGJ010000000
VERSION     DASUGJ010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098813"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="VFV93_17300"
     CDS             <1..1308
                     /locus_tag="VFV93_17300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEX5167166.1"
                     /translation="DPLQEGSGNFPPKLEDETLNPRLVTWLRLQLPTSALESDANAAE
                     AKVSWVGINAACIYQAVAVVNEVLGAGNGEPEQAFTLANLPVLPASIAIVAQKLDSGE
                     AEQWRLTDDLLAARVDERVFTLDPESGVVQFGDGLHGARPQPGWRVLASYEYGGGVRG
                     NVGIGAVKASRDTRLQGGFTISNPLPTSGGDLGESVDEAERSIPGYLRNRERLVTRED
                     FRDVTMRTPGDDMARVEVLTLFRPGNPPADDVPGVVTVMVVPRFDPVRPLWPTPDRLF
                     LQRVCDHLRPRRLVTTEVYVRGPIYVPVYISAGIQVRAGYFEDEVRATVRTVLNLYLS
                     ANPPGGPEQLGWPLNKRLMRKDLEAVVTRVPGVEFVESLELGVGSPVDIPDYDMTGLQ
                     LPVVVGLNVVTGVAEPLATVFGSPPPSDPGIRVVPIPVSKATC"
     gene            1331..3841
                     /locus_tag="VFV93_17305"
     CDS             1331..3841
                     /locus_tag="VFV93_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167167.1"
                     /translation="MDVNGTRFQLIKGQADWQAVLDETRRQGYEANLRWNATPEWLTL
                     NAPLSRFPLSRNEQGRLSSSRRGAAADQYGNWYWIADDERRIYWQPSGSRRPAAYWQQ
                     PSQPATPPGAFGPASTEAAQPATLRGLTVTTGHYLIVGNASRPGLLRFDLHAGGEPLL
                     VLIPPEIPFAPFDLAPSPDGGVWVLDRDHCRYWGFDRDMRAIGSETTPNEAVPPASLF
                     QPVGGMPLTPAPVAFPEGFPIAAQRPLAIEGLPDGTVLILDEAPDEQPTSVSTVYHYR
                     CDQQVSAPLPLRFEVEQIGSDGESTTVELPIAGHDLAYAACAERLYIADRGGKQVFAL
                     AAPLDAPLSVTTVERDYLPLHYFGSRALVAHDGAVYYDVVGGDVRNDANVRWIALQPL
                     DQPRFDRSATLVTPVLDGHARDCVWDRLYLDACIPAGATVAVWTRAHNDLDLLGDVPF
                     TREPDLYLRGMDAEVPYYDPFADLTTRSPATGTWELLFQRANGRFLQIQLDLSGNGRV
                     SPQLRALRAWYPRFSLSRRYLPAIYQEDAESGAFLERLLANPHGFFTLIEGQMRDVSV
                     LFDARSAPSDALDWLAGWVGLMLDPLWEQIQQRRQAGGAAVVAHDRRRLFIRFAMRLY
                     QWRGTAEGIQLALQLLLDPCLEITLQRFKDGSVLPNPGLAAELARLGLPYPTPVMSEE
                     AFEDLLRDYILSPRRPSKVRIVERFLTRGGRALLAGDASAEGQAAQQETVAATAHRFS
                     VLVPEDLSAEEAAMVERVVELEKPAHTLFDVRRYWDAFRVGEARLEIDTLLGESDRFR
                     QMILGRDYLAEGYLTPAYPMDIAERMISDRDGIQTPRL"
     gene            3869..>5239
                     /locus_tag="VFV93_17310"
     CDS             3869..>5239
                     /locus_tag="VFV93_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167168.1"
                     /translation="MGTTAEIAPLDPNKRVNYTFGMVLGVNDFRQEQEHFEWKQRTSN
                     LLLHGSGTVCGLHAAAELAAGGSDVQIRVTGGTAVSPRGNWISVNGDQCAQLNQWLQG
                     QQQQTGQPLPAGRQRIFVTLCYVECPTDLVPVAGQPCATDEDTRMPSRTLETFRLSFS
                     WEPPEQPREDIIRAFGDLLAQVEIDASLGSPPGADDSEVLLAAVRDLATLPLDASPPA
                     SPVEEGPFLLDAGSACETIQAALAIWTTEVCPSIPIPNAGDDCLLLACIEFDVDGSGS
                     LIFTTDTAGQLAPDSVVVSNCDRPILVPTRLQQELFCLLGGGSQQGPPGPPGRDGRDG
                     IDGRDGVDGRDGVDGQDGRDGVDGQDGQDGQDGQDGQDANVNLKSDRVDLIPGERTSL
                     DRFGSTDYTYKSNPIQHGLGQRPMISLAVDEIKFERLNFEGPEDPDSVDVALTVFQDP
                     GNDGFRI"
BASE COUNT          814 a         1780 c         1822 g          823 t
ORIGIN      
        1 gacccgctcc aggaaggctc cggcaacttc ccgccgaagc ttgaggacga gacgctgaat
       61 ccacggctgg tgacctggct gcggctgcag ctgccgacgt cggcgctgga gagcgacgcg
      121 aacgcggccg aggcgaaggt cagctgggtc ggcatcaacg cggcgtgcat ctaccaggcc
      181 gtcgccgtcg tgaacgaggt gctgggagcc ggcaacggcg aaccggagca ggcgttcacg
      241 ctcgccaacc tgccggtcct gccggcctcc atcgcgatcg tcgcccagaa gctcgacagc
      301 ggcgaggccg agcagtggcg cttgaccgac gacctgctgg cggctcgcgt agacgagcgc
      361 gtcttcacgc tcgatccgga gtcgggcgtc gtccagtttg gcgacgggct gcacggcgcc
      421 cgtccacaac cgggctggcg ggtgctggcc agctacgaat acggcggcgg cgtacgcggc
      481 aacgtcggca tcggcgccgt caaggccagc cgcgacacgc gcttgcaggg tggcttcacg
      541 atctccaacc cgctgccgac cagcggcggc gacctgggcg aatcggtcga cgaggcggaa
      601 cgctcgatcc ccggctacct gcgcaaccgc gagcggctgg tgacccgcga ggatttccgt
      661 gatgtcacga tgcggacgcc gggtgacgac atggcgcggg tcgaggtgct gacgctcttc
      721 cggcccggca acccgccggc cgacgatgtg ccgggggtcg taacggtgat ggtcgtgccg
      781 aggttcgacc cggttcgccc gctctggccg acgccggatc gactcttcct gcaacgtgtc
      841 tgcgaccacc tgcgcccgcg tcggctggta acgaccgagg tctacgtgcg cgggccgatc
      901 tacgtcccgg tctacatctc ggccggcatc caggttcggg ccggctattt cgaggacgag
      961 gtgcgggcaa ccgttcggac cgtcctcaac ctctacctgt cggccaatcc gccgggcggg
     1021 ccggagcagc tcggctggcc gctcaacaag cggctgatgc ggaaggacct cgaagcggtc
     1081 gtcacccgcg tgccgggtgt cgagttcgtc gagtcgctgg agctgggtgt cggcagtccg
     1141 gtcgatatcc ccgactacga catgacgggg ctgcaactac cggtcgtcgt tgggctgaac
     1201 gtcgtcaccg gcgtggccga gccgctggcg accgtcttcg gatcaccgcc gccgtccgac
     1261 cctggtattc gcgtcgtgcc gatcccggtc agcaaggcga cctgctgagg cgaagtgagg
     1321 gcaagctggc gtggacgtca acgggacgcg cttccaactg atcaaggggc aggctgactg
     1381 gcaggcggtg ctggatgaga cgcgccgtca gggctacgag gccaacctgc gctggaacgc
     1441 cacgccggag tggctgacgc tcaacgcccc gctgtcgcga ttccccctca gccgcaacga
     1501 gcagggacgg ttgtcgtcgt cccggcgtgg ggctgccgcc gaccagtacg gcaactggta
     1561 ctggatcgcc gacgatgagc ggcgcatcta ctggcaaccg tccggctcgc ggcgtccggc
     1621 cgcctactgg cagcagccgt cgcaaccggc gacgccgcct ggcgcgtttg gccccgcctc
     1681 gaccgaggcg gcacaaccgg ccaccttgcg cgggctgacg gtcacgaccg gccactacct
     1741 gatcgtcggc aacgccagcc ggccgggatt gttgcgcttc gacctgcacg cgggtggcga
     1801 gccgctgctg gtgctgatcc cacccgagat cccgttcgcg ccgttcgatc tggcgccctc
     1861 gccggacggc ggtgtctggg tgctcgaccg cgaccattgc cgctactggg gcttcgaccg
     1921 cgacatgcgc gcgatcggca gcgagacaac gccgaacgag gcggtgccac cagcctctct
     1981 gttccagccg gttggtggga tgccgctcac gcccgcgcca gtcgccttcc ccgagggatt
     2041 cccgatcgcc gcgcagcgtc cgctggcgat cgagggcttg ccggacggaa cggtcctgat
     2101 cctggacgaa gcgccggatg agcagccaac ctcagtctcg accgtctacc actatcgctg
     2161 cgaccagcag gtatcggcac cgttaccgct gcgattcgag gttgagcaga tcggcagcga
     2221 cggcgagtcg acgacggtcg agctgccgat cgccgggcac gacctggcct atgcggcctg
     2281 cgcggagcgg ctctacattg ccgatcgggg cgggaagcag gtcttcgcgc tggccgcacc
     2341 gctcgacgcg ccgttgagcg tgacgacggt tgagcgcgac tacctgccgc tgcactactt
     2401 cggatcgcgg gcgctggtcg cccacgacgg ggcggtctac tacgacgtgg ttggcggcga
     2461 tgtccgcaac gacgccaacg tgcgctggat cgcgctccag ccgctcgacc agccgcgctt
     2521 cgaccgctcg gcgacgctgg tgacgcccgt cctcgacggc cacgcgcgcg actgcgtctg
     2581 ggatcggctc tacctcgacg cctgcatccc ggccggcgca accgtcgcgg tctggacacg
     2641 ggcgcacaac gacctcgacc tgctcggtga cgtgccgttc acccgcgagc cggacctcta
     2701 cctgcgcggg atggacgcgg aggtgccgta ctacgatccg ttcgcggacc tgacgacgcg
     2761 ctcgcccgcg accggcacct gggaactgct cttccagcgg gcgaatggtc gcttcctgca
     2821 aatccagctc gatctgagcg gcaacgggcg ggtctcgccg cagttgcggg cgctgcgagc
     2881 ctggtatccg cgtttctcac tctcgcggcg ctacttgccg gctatctatc aagaggacgc
     2941 cgagtccggc gccttcctcg aacggttgct ggccaatccg cacggcttct tcacgctgat
     3001 cgagggccag atgcgcgatg tcagcgtcct gttcgacgcc cgcagtgcgc cgtccgacgc
     3061 gctcgactgg ctggccggct gggtcggact gatgctcgat ccgctctggg agcagatcca
     3121 gcagcgccgc caggcaggtg gcgctgccgt cgtcgcccac gaccgacgga ggctgttcat
     3181 ccgcttcgcg atgcgcctct accagtggcg cgggacggcc gaggggattc agcttgcgtt
     3241 gcaactgctg ctcgatccct gcctcgagat cacgcttcag cgtttcaagg atggctcggt
     3301 gctcccgaat cccggcctgg ccgccgagct ggcgcggctg gggctgccgt acccgacgcc
     3361 ggtcatgagc gaggaggcgt tcgaggacct gctgcgcgac tacatcctct cgccgcgccg
     3421 gcccagcaag gtccggatcg ttgagcgctt cctgacgcgc ggcggtcggg cgctgttggc
     3481 cggcgacgcc agcgcagaag ggcaggcagc gcagcaggag acggtcgccg caacggccca
     3541 ccgcttctcg gtcctcgtgc cggaagacct gtcggccgag gaagcggcca tggtcgagcg
     3601 ggtcgtcgag ctggagaagc cggcgcacac gctgttcgac gtgcggcgct actgggatgc
     3661 cttccgcgtc ggcgaggcac ggctggagat cgacacgttg ctgggcgaat ccgacaggtt
     3721 caggcagatg attctcgggc gcgactatct ggcggagggc tacctgactc ccgcctatcc
     3781 gatggatatc gcggagcgca tgatctcgga tcgcgacggc attcagacgc cgcggctgtg
     3841 accgttggag gcatcgatga gagactgcat gggcacgaca gcagagatcg ctccgctcga
     3901 cccaaacaag cgggtgaact acacgttcgg gatggtcctt ggcgtgaacg acttccgcca
     3961 ggagcaggag catttcgagt ggaagcagcg caccagcaac ctgctgctgc acgggtccgg
     4021 gacagtctgt ggtctgcacg ccgccgccga actggctgcc ggtgggagcg atgtccagat
     4081 ccgtgtgacg ggcgggacgg ccgtcagccc gcgcggcaac tggatctcgg tcaacggcga
     4141 ccagtgcgcg caactgaacc agtggctgca ggggcaacag cagcagaccg gccaaccatt
     4201 gccggccggc cgccagcgca tcttcgtcac gctctgctac gtcgagtgcc cgaccgatct
     4261 ggtcccggtc gccgggcagc cctgcgcgac cgacgaggac acgcggatgc cgtcgcgaac
     4321 gctcgaaacc ttccggctga gcttcagttg ggagccgccc gagcagccgc gcgaggacat
     4381 catccgcgcc ttcggcgacc tgctggcgca ggtcgagatc gacgcctcgc ttggctcacc
     4441 gccgggcgcg gacgacagcg aggtgctgct ggcggcagtc cgcgatctgg cgacgttgcc
     4501 actcgacgcc tcgccgccgg cctcgccggt cgaggagggc ccgttcctgc tcgacgccgg
     4561 cagcgcctgc gagacgatcc aggccgcgct ggcgatctgg acgaccgagg tctgcccgag
     4621 catcccgatc ccgaacgctg gcgacgactg cctgctgctg gcctgcatcg agttcgatgt
     4681 cgacggctcc ggcagcctga tcttcacgac cgacacggcc ggccaactcg cgccggacag
     4741 tgtcgtcgtc agcaactgcg accggccgat cctcgtcccg acccggttgc agcaggaact
     4801 gttctgcctg ctgggtggcg gcagtcagca ggggccacca gggccgccgg ggcgcgatgg
     4861 acgcgatggc attgacggac gggatggcgt tgatgggcga gacggtgttg atgggcagga
     4921 cgggcgcgac ggcgttgacg gtcaggatgg gcaggacggg caggacggac aagatgggca
     4981 ggacgcgaac gtcaacctga agtccgaccg agttgacctg attcctggcg agcgaacctc
     5041 gctggaccgc ttcgggtcga ccgactacac gtacaagtcg aacccgatcc agcacggtct
     5101 cgggcagcgg ccgatgatct cgctggccgt cgatgagatc aagttcgagc ggctgaactt
     5161 tgaaggcccg gaggaccccg actccgtcga tgtcgcgctg accgtcttcc aggaccccgg
     5221 gaacgacggg ttccggatc
//
LOCUS       DASUGJ010000766         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1099265, whole genome shotgun sequence.
ACCESSION   DASUGJ010000766 DASUGJ010000000
VERSION     DASUGJ010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099265"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFV93_17315"
     CDS             <1..192
                     /locus_tag="VFV93_17315"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5167169.1"
                     /translation="HPLTTRELEVVKGLVDGLSNAEIASVLFISPNTVSNHVSNIMNK
                     LGLDSRTAIATWAVRHGVG"
     gene            348..779
                     /locus_tag="VFV93_17320"
     CDS             348..779
                     /locus_tag="VFV93_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167170.1"
                     /translation="MQRTETARRHKHGRITKRTLNIAAIGMLVLGLAAVALTVELPAR
                     SDTESREIRAPQLTSDELYATEAAAAVAQVDGWQPTVGQLTSDEAWAIEASLVDAAEA
                     HRRAEAMQLTSDEAWALEQAQLLSRDSEQRSDHDFNPETAR"
     gene            complement(893..2488)
                     /locus_tag="VFV93_17325"
     CDS             complement(893..2488)
                     /locus_tag="VFV93_17325"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX5167171.1"
                     /translation="MATTLVGGTIVDGTGSPGREGNVVIDGDRIAGVGDVAPAGERID
                     VRGLVVSPGFIDTHSHCDLVCIADRQVAPKLRQGITTDLLGQDGLSEAPIKAEDVDRW
                     RTHLAGLNDDPQLDWTWRTYGEYFDRVDGAAINMAGMVGHGTVRLHVMGMDDRPPTAQ
                     ELTAIQALVDSAIQDGACGFSTGLIYSPCVYSDTEELIEIGKVVRRHNSFMVYHMRFE
                     GARVLDGMGEVFRIARESGAACHISHFKARGKLAWGKAPAMSASVNRARAEGLDITAD
                     QYPYTAGSTMLGALLPPWCHARGIEGLNAYLRDETIEGRIRAEIEGGRDDWESSIASS
                     GWDAIMISGVKTPANQWVVGKRIPEIAAGWALDNYQTMVRLLLDEQHAVSMILFMMDE
                     DDVRHLLVQPWLIHCTDGLMGGEPHPRTYGTYPKILGRYVREDGLMPLEEAIRKMTSL
                     PAWRLGMPDRGVLRTGAFADITIFNPETVIDTSTFEEPRQFPIGIEHVYVNGVPAVRD
                     GHETGNLAGRALRRQVGAPGTRR"
BASE COUNT          473 a          895 c          842 g          446 t
ORIGIN      
        1 cacccgctca cgacgcgtga gttggaggtc gtcaaaggcc tcgtcgatgg tctgtccaac
       61 gccgagatcg ccagcgtgct gttcatcagc ccgaacacgg tcagcaacca tgtctccaac
      121 atcatgaaca agctggggct ggattcgcga acggccatcg cgacctgggc ggtccggcac
      181 ggcgtcggct gacggcaggc cggcggcata gtaagcaatt actagtcaat cgcgagacgc
      241 gaatagtgaa gccgtagggt gtgcccgcgc cttcgcaagc acatgctgtg gatgtggttg
      301 acagtcggag atcagccatc agacagcaga ctcgaaggag cgcggatatg caacggactg
      361 agacggctcg acgacataag cacgggcgga tcacgaagcg gacgctgaac atcgcagcca
      421 tcggcatgct ggttctcggg ctggctgcgg tcgcgctgac cgtcgagctg cctgcgcgca
      481 gcgacacgga atcgcgagag atcagagccc cgcagcttac ctcggatgag ctgtacgcga
      541 cggaagctgc cgctgcagtc gcgcaggtag atggctggca accgacggtt gggcaattga
      601 cctcggacga ggcctgggcc atcgaagcgt cactcgtgga tgccgccgaa gcgcatcggc
      661 gtgctgaggc gatgcaactg acatcagacg aggcatgggc tctcgaacag gcgcagttgc
      721 tgagccgcga ttcggagcag cggtcggatc acgacttcaa cccggaaacg gcccggtaga
      781 cccaatcgct acgacgaaga acgcgctgct ggcactcgac gtcggcggcg cgttcgcgtt
      841 cgtcccgaca catcgagaag cagtgtggat acccaactgc cccgttggtc gatcagcgcc
      901 gcgtccccgg cgcgccgacc tgccgccgca gtgcccgccc agccaggttg ccggtttcgt
      961 ggccgtcgcg gacggccgga acgccgttga cgtagacgtg ctcgatcccg atcgggaact
     1021 ggcgcggctc ctcgaaggtc gaggtgtcga tcaccgtctc cgggttgaag atggtgatat
     1081 cggcgaacgc gccggttcgc aggacgccgc gatccggcat ccccagccgc caggccggca
     1141 gcgacgtcat cttgcggatc gcctcctcca gcggcatcag gccgtcctca cggacgtagc
     1201 gaccgaggat cttcggatag gtgccgtagg tgcgtgggtg cggttcgccg cccatcagcc
     1261 cgtcggtgca atggatcagc cacggctgga ccagcaggtg gcgaacgtcg tcttcgtcca
     1321 tcatgaatag aatcatcgag acggcgtgct gctcgtccag cagcagccgc accatcgtct
     1381 ggtaattgtc cagcgcccag ccggccgcga tctccgggat gcgcttgccg acgacccact
     1441 ggttcgcggg cgtcttgaca ccggagatca tgatcgcatc ccagccggac gaggcgatgc
     1501 tcgactccca gtcgtcgcgg ccaccttcga tctctgcccg aattcgcccc tcgatcgtct
     1561 cgtcgcgcag gtaggcgttc aagccctcga tccctcgtgc atggcaccac ggcggcagca
     1621 gcgcaccgag catcgtggat ccggccgtgt atgggtactg gtcggcggtg atgtccagcc
     1681 cctcggcccg cgcccggttg acggacgcgc tcatcgccgg cgccttgccc catgccagct
     1741 tgccgcgtgc cttgaagtgg gagatgtggc aggccgcgcc tgactcgcgg gcgatccgga
     1801 agacctcgcc catcccgtcc agcacgcgcg ccccctcgaa gcgcatgtga tagaccatga
     1861 acgagttgtg ccgccgaacg accttgccga tctcgatcag ctcctcggtg tccgagtaga
     1921 cgcacggcga gtagatcagc ccggtcgaga acccgcaggc gccgtcctga atcgcggagt
     1981 ccaccagcgc ctgaatggcg gtcagttcct gcgcagttgg cgggcggtcg tccatcccca
     2041 tcacgtgcaa tcggacggtc ccgtggccga ccatcccggc catgtttatc gccgcgccgt
     2101 cgacccgatc aaagtactcg ccgtacgtcc gccaggtcca gtcgagctgt ggatcgtcgt
     2161 tcagcccggc caggtgcgtt cgccagcggt cgacgtcctc ggccttgatc ggggcctcgc
     2221 tcaacccgtc ctgcccgagc aggtcggtcg tgatcccctg ccgcagcttt ggcgcgacct
     2281 gccgatcggc gatgcagacc aggtcacaat gcgagtgggt gtcgatgaac cccggcgaga
     2341 cgaccagccc gcgaacgtcg atccgctccc cggccggcgc gacatcgcca acgccggcaa
     2401 tccggtcgcc gtcgatgacg acgttccctt ctcgccccgg cgagccggtc ccgtcaacga
     2461 tcgtcccgcc aaccagcgtc gtagccatga aactgttctc ccctgtgtcc ctgaacacga
     2521 cactcaatgc tagcgtacgc agctgccgac cattcgacga ggacaacgtc cgttgccctg
     2581 tcatcctgag ccgcagcgaa ggatcgctta acgtgactga caggagcgcg ccgattggca
     2641 gaacgcgtcg tcgcta
//
LOCUS       DASUGJ010000767         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_544748, whole genome shotgun sequence.
ACCESSION   DASUGJ010000767 DASUGJ010000000
VERSION     DASUGJ010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544748"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..703)
                     /locus_tag="VFV93_17330"
     CDS             complement(80..703)
                     /locus_tag="VFV93_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167172.1"
                     /translation="MLAAIRSSINWPEKMDDAVRLYNRWATQAERQEVGFDGLIALGD
                     RDTGQGIAIALYDSAESLELSGQIAEEVLTATASLFAVPPIREIYDVIIHALPKRSGR
                     LDDDLEYARVTTIRVRPETIDQVFETVRTTVLANAQRQRGHRGFLGIGNRTTGKTMHF
                     TFWESESALYASETSGNYQNQVARIAHFLTEPPTREIYDVIVRRLPA"
     gene            complement(806..1192)
                     /locus_tag="VFV93_17335"
     CDS             complement(806..1192)
                     /locus_tag="VFV93_17335"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX5167173.1"
                     /translation="MARWRGILLLVCMLSVLLAACGSDDDDDDDAASNGAAASPAANT
                     IRIVTNDNVFEPEAVTAPAGEPVTVTFVNEGDNVHEVEIEGLVDETMLQPGESKDFTF
                     TPEKKEYRMYCEIHEDEGMEGTFTGE"
     gene            complement(1216..2058)
                     /locus_tag="VFV93_17340"
     CDS             complement(1216..2058)
                     /locus_tag="VFV93_17340"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX5167174.1"
                     /translation="MNVGAAVLTFREGLEAALIVAIMLTYLRKVGRPDAGRFVWIGAG
                     SAVALTIGFVGLLQIIGAEFEYPEKGVFEGVTGVLAVAMVTYMTFWMSRQGRQMKGHL
                     ESGVRESLAEGGRWGLFTLVFLAVIREGIETGLFLSAATFASSGTQTLVGGIVGLAIA
                     IVVAVAIYAGGVRLNLGRFFQIAGILLVVFGAAMLRYSVHEFEEVGLIPPMIERIWYT
                     GRELPEGTGVGAVLQALVGYTSKPSLSQLLAFVGYYLVVGLALWRPWRRWTASGTVAQ
                     TTVA"
     gene            complement(2263..>3001)
                     /locus_tag="VFV93_17345"
     CDS             complement(2263..>3001)
                     /locus_tag="VFV93_17345"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5167175.1"
                     /translation="ANAAQLWASIPDLRFEIVSVDQGSGGMVSAEWMMLGTNHGSMFG
                     LPPTGNPIELPGADFIRIEDERILSVTGYFDSGLLPRQLGMQVLVQPHTAGPFTFGRA
                     LAVHGERTDQPGAFGITILSSADVADQERISEYSRQIAVEMLGMDGFLGWFGAVVGDR
                     MATITAWESPEHAAQLMKGGAHREAMKFFWADNSVSSGGWTSVWKPERINTVWARCTA
                     CGKMVNSERLNGVCECGETLPSFGPWW"
BASE COUNT          570 a          957 c          931 g          543 t
ORIGIN      
        1 gcagcctcgc cctgtgtgga tcgcaacgga caagggcgag gctgcggcca tcgcccctac
       61 gcgagggaat ggctgatccc tacgccggca ggcgccgcac aatcacatcg tagatctcgc
      121 gggtgggggg ttcggtgagg aagtgggcga tgcgggcgac ctggttctgg tagttgccgc
      181 tggtttcgct ggcgtagagg gctgattcgg attcccagaa ggtgaagtgc atcgtcttgc
      241 cggtcgtgcg gttgccgatg ccgaggaatc cacggtggcc gcgttggcgc tgggcgttgg
      301 cgaggacggt cgtccgaacg gtttcgaaga cctggtcgat cgtctccggt cggacacgaa
      361 tggtcgtcac ccgcgcgtat tcaaggtcgt catcgagccg gcccgagcgc ttcggcagcg
      421 cgtggatgat gacgtcgtag atctcacgga tcggcgggac ggcgaacagg ctggctgtcg
      481 ccgtcaggac ctcttcggca atctgccccg atagttccag cgactcggcc gaatcgtaca
      541 gggcaatcgc gatcccctgg ccagtgtcgc ggtccccgag ggcgatcagc ccgtcgaagc
      601 cgacttcctg ccgttcggcc tgtgtcgccc agcggttgta gagacgaacg gcatcgtcca
      661 tcttttctgg ccagttgatc gaggagcgaa tggctgccag catatcgacc tccgatgagc
      721 gcgcggcgtg cctcgattgc gtcgatggat aatgccagat tgcgaaggca accgcgctgc
      781 ggaggatatg agatgaccgg taccgctact cgccggtaaa tgttccttcc atgccctcgt
      841 cctcatgaat ctcgcaatac atgcgatact ctttcttctc cggcgtgaaa gtgaagtctt
      901 tgctctcacc cggctgcagc atggtttcat cgacgaggcc ctcaatctcg acctcgtgga
      961 cgttgtcgcc ttcgttgacg aatgtcaccg ttaccggttc gccggccggc gccgtcacgg
     1021 cctccggctc gaagacgttg tcgttggtaa cgatgcgaat cgtgtttgcg gccgggctgg
     1081 ctgcggcacc gttggacgct gcatcgtcat cgtcgtcgtc atcgctcccg caggcggcaa
     1141 gcagcacgct caacatacac acgagcagca gaattccgcg ccatcgagcc atctatcggt
     1201 tcctttcggg catacctagg ccacggttgt ctgtgcgacg gtgccggagg cagtccagcg
     1261 tcgccatggt cgccagagcg caaggccgac gaccaggtag tagccgacaa acgcgagcag
     1321 ctggctaagt gacggcttcg acgtgtagcc gaccagtgcc tgcaggacgg cacctacacc
     1381 ggtcccttca ggcaactcgc gccccgtgta ccagatgcgc tcgatcatcg gcgggatcag
     1441 cccaacttct tcaaactcat ggacgctgta gcgcaacatt gccgcgccga agacgacaag
     1501 cagaatgccg gcaatctgga agaagcggcc taaattcagg cgaactccac cggcgtagat
     1561 cgcgaccgcc acgacgatcg cgatggccag gccgacgata ccgccaacga gtgtctgggt
     1621 gccggatgag gcgaatgtcg ccgctgagag gaagaggccg gtctcaattc cctcacggat
     1681 gacggccagg aaaacgagcg tgaacagtcc ccaacgcccg ccttcggcca gtgattcgcg
     1741 aacgccggac tctagatggc ccttcatctg gcgcccttgt cgcgacatcc agaaggtcat
     1801 gtaggtgacc atcgcgacgg ccaggacgcc ggtgacacct tcaaagacgc ccttctcggg
     1861 atactcgaac tcggcgccga tgatctgcag caggccgacg aaaccgattg tcagcgctac
     1921 agcgcttcca gctccgatcc agacgaaacg cccagcatcg ggacggccaa ccttgcgtaa
     1981 gtaggtcagc atgatcgcga caatcagtgc cgcttccaat ccctcgcgga acgtcaacac
     2041 ggccgcgcct acgttcatcc gctacctttc cctcacgatg cccaatccgt agacggtggg
     2101 ccactattcc cgactcattg agtcggaatt atcaaccaga ggatagggag cgggtgagaa
     2161 agtgtcaagg tgtcggtcag cagagcacag tcatcacccg gctgactgtc gccgattgag
     2221 caacaacggc gccggcgacc gcctgtcacc tcatcgttcg cgctaccacc atggcccgaa
     2281 cgacggcagc gtttcgccgc actcgcagac gccattcagc cgttcgctgt tgaccatctt
     2341 gccgcaggcc gtgcagcgcg cccacaccgt gttgatccgc tccggcttcc agaccgaggt
     2401 ccagccgccc gaagaaacgc tgttgtcggc ccagaagaac ttcatcgcct cgcggtgggc
     2461 gccgcccttc atgagctggg cggcgtgttc gggcgactcc caggcggtga tcgtcgccat
     2521 ccggtcgcca acgacggccc cgaaccagcc gaggaagcca tccatcccga gcatctcgac
     2581 ggcgatctgg cggctgtact cggagatccg ctcctggtcg gcgacgtcgg cactggagag
     2641 gatcgtgatg ccgaacgcgc cgggttggtc ggtgcgctcg ccatgcaccg caagcgcgcg
     2701 tccgaatgta aacggcccgg ccgtgtgcgg ctgaaccagc acctgcatcc cgagctggcg
     2761 cgggagcaga ccggaatcga agtagccggt gactgacagg atcctctcgt cctcgatgcg
     2821 aatgaaatcc gcgccgggta gctcaatcgg gttgccggtc ggcggcaggc cgaacatcga
     2881 gccatggttc gtgccgagca tcatccactc ggcggagacc atcccgccgg agccctgatc
     2941 gacgctgacg atctcgaagc gcagatcggg aatcgacgcc cagagttgag cggcattggc
     3001 g
//
LOCUS       DASUGJ010000768         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2072460, whole genome shotgun sequence.
ACCESSION   DASUGJ010000768 DASUGJ010000000
VERSION     DASUGJ010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072460"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /locus_tag="VFV93_17350"
     CDS             <1..1759
                     /locus_tag="VFV93_17350"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5167176.1"
                     /translation="TAASRLEAAARMLQQRGADPREYYLLPFEIGSILRYASSPNVTS
                     YLADASAAARQAGDAAVAAIADAELGYNHLLQGDVQRGIGEIERALAECRRLLRADPT
                     VFDRAAVEFSLSAEATTLADRLAARTAILLLAGAFVGSLDQTLTAVEQFGTTVDEVFQ
                     RDATVPYGLTANGIPYQLADLTWAIAMTDALLGRVADAQRRFSYAEQIYTNEAEHYLV
                     MGVNFWSLTRVELPYLADNPAATHARADAARAALSRSLNVAPMDTLDAWPDIPLMLID
                     GRWDACRAMATNIRGRTGFALNEHVIAGILATIACHQGDADDAWREILTVLPDGPATA
                     PGNTAFLSALTLQRVAVWLSIGADDFETTEGWLEAQDRWLSWSGAVLGHAENQLLWAD
                     YHHRRGRSDQARTYATRALHAASDPRQPLELVTIHRFIGRLERESRHLIQAEEHLLES
                     LRLAQACAAPFERALTLLELAQLRVVQQRGDDARRLLAEARALSEPLHARPTLERIAA
                     LEANLAASEKPDYPAGLSAREVEVLRLVSEGLTDAEVAERLFLSRRTVSTHLTSIYTK
                     LGVNSRTAATRFAVDHGLT"
     gene            2001..2861
                     /locus_tag="VFV93_17355"
     CDS             2001..2861
                     /locus_tag="VFV93_17355"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5167177.1"
                     /translation="MTIDAGLATRSANTGSADWQPDAVESSHSSRRAYLETVFETPAI
                     RLMLERTIELLQLRPGQSILEVGCGPGVILPRWAELVGPGGRVVAIDHAESFVADARD
                     RVAGLDLGDIVTVDLGDAYALPYDDQCFDIAHCERVLMHLADPQAALQEMRRVVRPGG
                     RVVAVEPDWQMIPFDHSDPEAMMALYAQHLKHIRQPDMGRTLYRRFGECGLTDRQVVP
                     VVYPITDFSVLRTYGLYPEHHLDAVAANGGISRDRAIAAIDQLETASRDELFFTVAIG
                     FAVAGIVPEA"
BASE COUNT          545 a          997 c          964 g          543 t
ORIGIN      
        1 gacggcggcc agtcgcctgg aagcggccgc caggatgctt caacagcggg gcgctgatcc
       61 ccgggaatac tacctgctcc ccttcgagat cggatcgatc ctgcgttacg cgtcctcgcc
      121 gaatgtcacg tcctacctgg cggacgcgtc ggcggcagcg cggcaagcag gagacgcggc
      181 cgtcgcagcg atcgcggacg cagagctggg ctacaaccat ctgttgcagg gcgacgttca
      241 acgcggcatc ggagagatcg agcgggcact tgcggaatgc cggcgactcc tgcgtgccga
      301 cccgaccgtc ttcgatcgcg ccgcggttga gttcagtctg agcgccgagg cgaccacctt
      361 agcggacagg ctggctgccc ggacggcgat cctcctgctc gcgggcgcct tcgtgggttc
      421 cctggaccag actctgactg cggtagagca gtttggcaca acggttgacg aggtctttca
      481 gcgcgatgcc accgtaccct atgggctgac ggcgaacggg ataccgtacc aactggccga
      541 tctgacctgg gccatcgcga tgaccgacgc actgctcggc cgggttgccg acgcccagcg
      601 ccgcttcagc tatgcggagc agatctatac caacgaggcg gagcactacc tggtcatggg
      661 cgtcaatttc tggtcactga ccagagtcga actcccctat cttgccgaca atccggcggc
      721 aacccacgca cgcgccgacg cggccagggc cgcgcttagc cggtcgctca acgtcgcacc
      781 aatggacacg ctcgacgcct ggccggacat tccattgatg ctcatcgacg ggcgctggga
      841 cgcctgccgc gcgatggcca cgaacatccg gggcagaacg gggtttgcct tgaatgagca
      901 tgtgatcgcc ggaatcctgg cgacgatcgc ctgtcatcag ggggacgccg acgacgcgtg
      961 gcgtgagatt ctgaccgtgc tcccggatgg accagcgacc gcgccgggca atacggcatt
     1021 cctctcagca cttacgctgc aacgagttgc cgtctggttg tccatcgggg ccgatgattt
     1081 cgagaccaca gaagggtggc tggaggcgca ggatcgctgg ctcagttggt ccggcgcggt
     1141 gctggggcac gccgagaacc agttgctctg ggctgactat caccatcgac gtggccgctc
     1201 agatcaggca cgcacatatg cgacccgtgc actccacgct gcctcagacc cacgtcagcc
     1261 gttggagctg gtaacgattc accgcttcat cggccggctc gaacgcgagt cccgtcacct
     1321 catccaggcc gaggagcatc tgctggaatc gttgcgactt gctcaggcgt gcgctgcccc
     1381 gttcgagcgt gcgctcaccc tgctggaact cgcgcaactg cgagttgtgc agcagcgggg
     1441 tgacgacgca cggcggttgc tggcggaagc cagggcgttg tctgagccac tgcacgctcg
     1501 tccaacgctg gagcgaatcg cagcgttgga agccaatctc gccgccagcg agaagccaga
     1561 ctacccggcc ggcctgagcg cccgcgaggt cgaggtgctg cggctggtgt ccgaaggact
     1621 gaccgacgcc gaggtcgccg agcggctctt cctcagccgg cggaccgtca gcacccacct
     1681 gacttcgatc tacaccaagc tcggcgtcaa ttcgcgaacg gccgccaccc gcttcgccgt
     1741 tgaccacggc ctgacctgac atcgaaccgg cttcatcccc ggccagtcgt cgcggtttct
     1801 gccggccacg atcgcgtacg tatcttgctg ggggaaacgc ctcgcggcga ttacgtactc
     1861 ctctggatgt ttcaacacca cccactcact atcctcgtct catcacaact gaaacgcggg
     1921 tcactcgcac gagcaggcac ggaacgacgg acgatcggcg ttcctggttc ctgccggcct
     1981 gcatcacgaa aggcaccgag atgacgattg acgcaggatt agctacccga tcggcaaaca
     2041 ccggcagcgc ggactggcaa cccgatgccg ttgaatccag ccacagcagt cgtcgcgcgt
     2101 acctcgagac ggtcttcgag acgccggcca tccgtctgat gctggaaagg acgatcgaac
     2161 tccttcagtt gcgccccggt cagtcgatcc tggaagttgg atgcgggccg ggcgtgatcc
     2221 tgccgcgctg ggcagaactc gtcggcccgg gcgggcgcgt cgtggcgatc gaccacgccg
     2281 agtcgttcgt cgccgatgcc cgggatcgcg tggcaggtct cgacctgggc gacatcgtca
     2341 ccgtcgatct gggcgacgcg tatgcattgc cctacgacga ccagtgcttc gacattgctc
     2401 actgcgaacg ggtgttgatg catctggctg acccgcaagc ggcgttgcag gagatgcgcc
     2461 gggtcgtccg gccgggcggc cgggtcgtcg ccgtcgagcc ggactggcag atgattccgt
     2521 ttgaccactc cgatccggaa gcgatgatgg cgctctatgc gcagcacctg aagcatattc
     2581 gtcaaccgga tatgggacgc acgctctatc gacgcttcgg tgagtgcggg cttaccgacc
     2641 ggcaggtcgt gccggtcgtc tatccgatca ccgatttttc tgtcctgcgg acatatggct
     2701 tgtacccgga acaccatctg gacgcggtag ccgccaacgg aggcatctcc cgggaccgag
     2761 cgatcgcggc aatcgaccaa ctcgagaccg caagtcgaga cgagctgttc ttcacggttg
     2821 cgatcggttt tgcggtggca ggcattgtcc cggaagcctg accacgtact cacgagtcat
     2881 tcttcaacat catccgacgt agcacgacga agtcgttgct caacgtcgct atcgacaccg
     2941 aagacggacg cgatcaggtg aggtcgatca ctacgccctc gtccgccgcc agtgcgaccg
     3001 tcccggcgac gtggttgtcg agccggttgg gattggtcga gagcagcac
//
LOCUS       DASUGJ010000769         5863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_961485, whole genome shotgun sequence.
ACCESSION   DASUGJ010000769 DASUGJ010000000
VERSION     DASUGJ010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961485"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..892)
                     /locus_tag="VFV93_17360"
     CDS             complement(167..892)
                     /locus_tag="VFV93_17360"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5167178.1"
                     /translation="MSVAAWSVRELAEQAGTTVRTIHYYISEGLLPPPAGATRSATYS
                     EAHLARLRLIGALRDEGLALASIRQRLAPLSDEQALDVVADLDEHLAEPEATPLTTLG
                     LIEAALASRAMEEEVTSQMTTVSRVMPSPATAPYSAEVAPSDRADSASEYLKRVLRKP
                     APRPEQPVPMPRPMPRPAPPQRPQLDTSRPETWHYYQIEDGIELRVREDRYRESKGRL
                     LAVIDTLKTSLRRYGLADPRNDV"
     gene            1085..1576
                     /locus_tag="VFV93_17365"
     CDS             1085..1576
                     /locus_tag="VFV93_17365"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5167179.1"
                     /translation="MERELDDATAGVGAVDVTGSRIAPVLTRWRHGFGWTQEELATRA
                     GISRGYLSRLERGLPGRPGLDILSRVCGALGRTWTELFSEAGLSLPGDVTLADIGDGL
                     HDPELMLYLRRLPELDGRDRAVLRAVLRAFFEREAADRTALPANALQLALPETTRLIT
                     DGS"
     gene            1664..2068
                     /locus_tag="VFV93_17370"
     CDS             1664..2068
                     /locus_tag="VFV93_17370"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HEX5167180.1"
                     /translation="MPTRIDSCPSCNGPFEIRELYCPECDVQIRGHFAPPEPSTFARL
                     NDEQEAFLRLFVLSRGNLSDVERSLGVSYPTVRAKLDDLIAAVSESQDEPATQAAPEP
                     PLTRSEILARVSDGRMSADEGMTMLQQLASEA"
     gene            2073..3338
                     /locus_tag="VFV93_17375"
     CDS             2073..3338
                     /locus_tag="VFV93_17375"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5167181.1"
                     /translation="MAAELSRAIPVTTNRAPIYALLLASAISNVGNMLTMIALPWFVL
                     ETTGSAAMTGVVGFFVALPNFLAGMFGGTLVDRLGYKRVSIIADLVSGFGVLMVPLLY
                     LTTGIAFWQLLVLVFIGALLDIPGITSRRAMLPELTRLGGLRLEQVNSAFESVQYLAL
                     LLGPPLAGVLIGWLGAQNVLWLDAATFAVSALLVAVAVPAPVQAARAAGGRYLDELKA
                     GLRFLRADQVLFSMAVAVGLSNFLGASTLSVIYPVFVKEEFGRASVLGILAAAFGVGG
                     LLGAVIYGLYGHQIPRRAMWITSFLAFPFTLLAPLLTSSLPVLLTILFLTSIVAGPIN
                     AMMVTVRHERIPQELRGRVFSTFSAITAVATPLGILLSGYLVEAVGLDATLLTIVVPE
                     FALAAATVVLPAFHLMDATRGRSVHGEAA"
     gene            3335..4621
                     /locus_tag="VFV93_17380"
     CDS             3335..4621
                     /locus_tag="VFV93_17380"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5167182.1"
                     /translation="MSSINRRRLPLYGLLTANLISRVGNNLTNIAIPWFVLATTGSAA
                     KTGLVAAAGILPIIVTGVLGGPLIDRLGYKRTAVISDVASGVNVALIPLLYSTVGLPF
                     WALLTLVLLGAVLDSPGNAARVSLMPDLAGLAAMPLERANSIIMIAGRTSAMLGAPLA
                     GLLIATIGSSNLLWIDAISFGISAVVLVTTIPNRVAAAVSETAVQAGISLRAYLAEII
                     DGFRFMRRDGLILWLTVGFSLGSLLAEPVYSVIMPVYAKEVYGSALDLGLMYSALGAG
                     SILGATLFAVFGHRMPRRATMLTGFTVRALSFWVLVAMPPIGIVVAAIVINATALEPT
                     NPMIQTIYQERIPDGMRGRVFGTVMAIGVATAPVGMIGYGLLIGHVGLQTSLYIFAAI
                     NLTLPLMLALMPAFRLLGSPAGRPGATSPGQPALPV"
     gene            4644..5423
                     /locus_tag="VFV93_17385"
     CDS             4644..5423
                     /locus_tag="VFV93_17385"
                     /inference="COORDINATES: protein motif:HMM:NF028425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HEX5167183.1"
                     /translation="MIFGRNSELSREDQIDLQDATTARVKLDMGVGSLKLRGGAGNLM
                     DARFEYNEGMEPRVDYVVHNGRGELRVDQPSRPKSFNITRNRWDVRLSESLPIDLEID
                     NGAGEADVDASALALTSFELDQAAGEGGVKLNGDQSRLTYVDADISAGKLELEMRGNY
                     PAMRELRAETAAGQLKLDLTGQWSSDVDVKIEVVAGEATLRLPGNVNIIATATTSVGR
                     VKARGLVQDGERYTREVPGAAGTLRIKANASVGQIVLEVAN"
     gene            5427..>5863
                     /locus_tag="VFV93_17390"
     CDS             5427..>5863
                     /locus_tag="VFV93_17390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5167184.1"
                     /translation="MTAGRSLSTSRIPLLALLAANAVSMTGNVFTLIAIPWFVLQTTG
                     SASQTGLTSAVAAIPFVIAGLFGGTIVDRVGFRRMSIIADLASSVTTALIPLLYVTVG
                     VEFWQLLVLVLLGGLLDTPGTTARAALLPDLAQRAGMRFERVNA"
BASE COUNT          869 a         2068 c         1815 g         1111 t
ORIGIN      
        1 aatcgttggt tcagccatcg cggggctccc ttcaaccgtc gccgttttcg gaggcgacat
       61 catccaggcc gcgcgccacc gcgcgacatg acgatcagcg aatgctcgat tccgtgtgag
      121 cgcgatggaa acgggaaccg atcccattcc cccggtggag cgcccgctag acgtcgttgc
      181 gagggtccgc cagcccgtat cggcgcagcg aggttttcaa tgtgtcgatc accgccagca
      241 gccggccttt cgactcccgg tagcgatcct cgcgtacgcg caactcgatc ccgtcttcaa
      301 tctgatagta gtgccaggtc tccggccggc tcgtgtcaag ctgtggccgt tgcggcgggg
      361 ccggtcgcgg catcggcctc ggcatcggaa ccggctgctc cggccgaggt gccggcttgc
      421 gcaggacgcg cttcagatac tcgctggctg aatcggcccg gtccgatggc gcaacctcag
      481 cagaatacgg cgcggttgcg gggctgggca tcactctcga cacggtcgtc atctgcgacg
      541 tcacttcctc ttccatggcc cggctcgcca gtgccgcctc gatcagtccg agcgtggtca
      601 acggcgtcgc ctccggttcg gccagatgct cgtcgaggtc ggcgacgacg tccagcgcct
      661 gctcgtccga gagcggcgcc agtcgctgcc tgatactggc cagcgccagc ccctcgtcac
      721 gcagggcgcc aatcaaccgc aagcgcgcca gatgggcctc tgaataggtc gcgctccgcg
      781 tcgccccggc cggcggcggc aacagcccct cactgatgta gtagtgaatc gtccgcacgg
      841 tcgttccagc ctgctcggcc agttcgcgaa cactccaggc tgctaccgac atacgctcat
      901 ccatcaatcg ctcccacaat tcggagcaga taatatcagt accacgcgct actgtcaata
      961 atgcgtttgc aactggtgaa cccaagccgc catgtacccc catcgctcac cccaggtcac
     1021 gcgctacgac cacccctgcg gtatcctctc cagcatcgct tcgacgtacg ttgggagggc
     1081 tgccgtggag cgtgaactgg acgatgcgac ggccggcgtc ggcgccgttg atgtcaccgg
     1141 cagccggatc gcgccggtac tgacgcgctg gcgacatggc ttcggctgga cccaggagga
     1201 gctggcgacg cgggcgggga tctcgcgcgg ctacctctcg cgcctcgaac ggggtctgcc
     1261 gggccggccg ggactcgaca tcctctcgcg cgtttgtggc gcgctcggcc ggacctggac
     1321 ggagctgttc agcgaggccg gtctctcgct gcccggcgat gtcacgctgg ccgacatcgg
     1381 cgacgggctc catgatcctg agctgatgct ctacctacgc cgcctgccgg aactcgacgg
     1441 ccgcgatcgc gccgttctgc gtgccgttct gcgtgccttc ttcgagcgcg aggccgccga
     1501 ccgcaccgcg ctgcctgcca acgccctcca actggccctg ccggagacga cgcggttgat
     1561 cactgatggt tcatgaaagg gtctgtttct aaactgattt tgaaattctg ttgacaaacg
     1621 gtacagcacc acgtatcatc ctccacgtta ggagggtgaa tacgtgccaa cgcgaatcga
     1681 ttcctgccca tcgtgtaacg gcccattcga gattcgggaa ctctactgcc ccgaatgtga
     1741 tgtccagatc cgcggccact tcgcgccgcc cgagccgtcg acgtttgcgc gactcaacga
     1801 cgagcaggaa gccttcctgc gcctcttcgt cctctcccgc ggcaatctct cggatgtcga
     1861 gcgctcgctc ggcgtctcct acccgaccgt ccgcgccaag ctggacgatc tgatcgccgc
     1921 cgtctccgag tcgcaggacg agccggccac ccaggccgcg cccgagccgc cgctgacccg
     1981 ttccgagatt ctcgcccgcg tctccgacgg acgcatgagc gctgacgagg gtatgacgat
     2041 gctccagcag ctggcgtcgg aggcctgagt cgatggctgc cgaactgtcc cgcgcgatcc
     2101 cagtgaccac caaccgggcg ccgatatacg ccctgttgct tgccagtgcg atctcgaacg
     2161 tcggcaatat gctgacgatg atcgcgctgc cctggttcgt gctggagacg actggcagcg
     2221 cggcgatgac cggtgtcgtc ggattcttcg ttgccctgcc caatttcctg gccggtatgt
     2281 tcggcggcac gctcgtcgat cgcctcggct acaaacgtgt cagcatcatc gccgacctcg
     2341 tcagcggctt cggcgtcctg atggtccctc tgctctacct gacgaccggg atcgccttct
     2401 ggcagttgct tgttctcgtc ttcatcggcg cgctactcga catccccggc atcacttccc
     2461 ggcgcgcgat gctgccggag ctgacccgtc tgggcggctt gcggctggag caggtcaact
     2521 ctgctttcga gtcggtccag tacctcgcgt tgctgctggg gccgccgctg gccggtgtcc
     2581 tgatcggctg gctcggtgcc cagaacgtgc tctggctcga tgccgcgacc ttcgccgttt
     2641 cggcgctgct cgtggcggtc gcggtgccgg ctccggttca agctgcccgc gccgccgggg
     2701 gtcgctacct tgacgagctg aaagccgggc tgcgcttcct gcgcgccgac caggtgctgt
     2761 tctcgatggc ggtcgcagtc gggctgagca acttcctcgg cgcgtcgacg ctctcggtca
     2821 tttacccggt cttcgtcaag gaagagttcg gccgggcttc ggtgctcggc atcctggcgg
     2881 cggcctttgg cgtcggcggc ctgctcggcg ccgtcatcta cggcctctac ggccaccaga
     2941 ttccgcgccg cgcgatgtgg atcaccagct tcctggcctt cccgttcacg ctgcttgcgc
     3001 cgctgctgac atcgtcgttg ccggtgctgc tgaccatcct gttcctgacc tcaatcgtcg
     3061 ccggcccgat caacgcgatg atggtcaccg ttcgccatga gcgcatccca caggaactgc
     3121 gcgggcgcgt cttcagcacc ttctcagcta tcacggctgt cgcgacaccg ctcggaatcc
     3181 tgctctccgg ctacttggtc gaagcggtcg gtctcgacgc cacgctcttg acgatcgtcg
     3241 tccctgaatt cgcgctcgct gctgccaccg tcgtcctgcc cgccttccat ctgatggatg
     3301 caaccagggg acgttccgtc cacggggagg cagcgtgagc agcatcaacc ggcgccgcct
     3361 gccgctctac gggctgctga ccgccaatct catctcgcgg gtcggcaaca acctgaccaa
     3421 catcgccatt ccctggttcg tgctggcgac gaccggtagc gcggccaaga ccggcctcgt
     3481 cgcggccgcc ggcatcctgc cgattatcgt caccggcgtc ctcggcggcc cgctgatcga
     3541 ccggcttggt tacaagcgga cggccgtcat ctcggacgtc gccagcggcg tcaacgtcgc
     3601 cctgatcccg ctgctctact cgaccgtcgg cctgccgttc tgggcgctgc tgacgctcgt
     3661 cctgctgggc gccgtcctcg actcgcccgg caacgctgcc cgcgtcagtc tgatgccgga
     3721 cctggccggt ctcgccgcga tgccgctcga acgggccaac tcgatcatca tgatcgccgg
     3781 ccggacctcg gcgatgctgg gtgcgccgct ggccggtctg ctgatcgcga cgatcggctc
     3841 cagcaacctg ctctggatcg acgccatcag cttcggtatc tcggccgtgg tcctcgtgac
     3901 aacgatcccg aaccgcgtgg cggccgccgt ttccgaaacg gctgtccagg ccggcatctc
     3961 gctgcgcgcc tatctcgcgg agattatcga cggcttccgc ttcatgcgcc gcgacggcct
     4021 gatcctctgg ctgacggtcg gcttctcgct cggcagcctg ctggccgagc cggtctactc
     4081 agtcatcatg ccggtctacg ccaaggaggt ctacggcagc gcgcttgacc tcggcctcat
     4141 gtactcggcg ctcggtgccg gctcgatcct cggcgcgacg ctgttcgccg tcttcggcca
     4201 ccggatgccg cgccgcgcga cgatgctgac cggcttcacg gttcgcgcgc tcagcttctg
     4261 ggttcttgtt gccatgccgc cgatcggcat cgtcgtcgct gcgatcgtca tcaacgcgac
     4321 cgcgctcgaa ccgaccaacc cgatgatcca gaccatttac caggagcgca tccccgacgg
     4381 catgcgcggc cgcgtcttcg ggacggtgat ggcgattggt gtcgcgaccg ccccggtcgg
     4441 gatgatcgga tacggactgt tgatcggcca tgtcggcctg caaaccagcc tctacatctt
     4501 cgcggcgatc aacctgacgt taccgctgat gcttgccctg atgcctgctt tccgcttgct
     4561 tggatcgcct gccggacgtc ccggcgcgac ctcacccggc cagccggccc tgccggtctg
     4621 agccaacgcg aaaggaacga ccgatgatct ttggacgcaa ttccgaactc tcccgcgagg
     4681 accagatcga tctgcaggac gcgacgacgg cccgcgtcaa gctcgacatg ggtgttggct
     4741 cgctcaagct gcgcggtggc gccggcaacc tgatggatgc ccgcttcgag tacaacgagg
     4801 gcatggagcc gcgcgtcgac tacgtcgtcc ataacggacg tggcgagctg cgcgttgacc
     4861 agccgagccg cccgaagtcg ttcaacatca cccgcaaccg ctgggatgtt cgcctcagcg
     4921 aaagcctgcc gattgacctg gagatcgaca acggggccgg cgaggccgac gtcgatgcct
     4981 cggcgctcgc gttgaccagc ttcgagctgg atcaggcggc cggcgagggt ggggtcaagc
     5041 tgaacggcga ccagtcccgg ctgacctatg tcgatgccga catctcggcc ggcaagctcg
     5101 agcttgagat gcgcggcaac tatcccgcca tgcgtgagct gcgcgccgag accgctgccg
     5161 gtcagctcaa gctcgacctg accggccagt ggagctccga cgtcgatgtc aagatcgagg
     5221 tcgtcgccgg cgaggccacg ctgcggctac cgggtaatgt caacatcatc gccaccgcca
     5281 cgaccagcgt cggccgtgtc aaggcacgcg gcctcgtcca ggatggcgaa cgctatacgc
     5341 gtgaggtgcc cggcgcagcc ggcacactgc gcatcaaggc caacgccagc gtcgggcaga
     5401 ttgtcctgga agtggcgaac tgattcatga ctgccggccg gtcgctcagc accagtcgaa
     5461 taccgctctt ggccctgctg gccgccaacg cggtctcgat gacgggcaac gtcttcacac
     5521 tgatcgcgat cccgtggttc gtgttgcaga cgaccggcag cgcctcgcag accggcctga
     5581 ccagcgccgt cgccgccatc ccgttcgtga tcgccggtct gttcggcggc acaatcgtcg
     5641 atcgcgtcgg attccggcgg atgagcatca tcgccgacct ggccagcagt gtcacgacgg
     5701 cgctgatccc gctgctctac gtgacggtcg gggtcgagtt ctggcaactg ctggtgctcg
     5761 tcctcctcgg cggcctgctt gatacgcccg gcacgaccgc ccgcgccgcg ctgctgcccg
     5821 atctcgccca gcgggccggg atgcgcttcg agcgggtcaa cgc
//
LOCUS       DASUGJ010000770         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_822008, whole genome shotgun sequence.
ACCESSION   DASUGJ010000770 DASUGJ010000000
VERSION     DASUGJ010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822008"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="VFV93_17395"
     CDS             complement(<1..1686)
                     /locus_tag="VFV93_17395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5167185.1"
                     /translation="MPETTERTAPRLLLDQRVTMRDGVELSADVYLPPTGDGPWPAIL
                     QRTPYDNTAALWVNIAVYFAQRGYAFVSQDVRGRCDSGGEWEPFVNEGPDGHDTIEWI
                     AEQEWCDGKVGMMGGSYGGFVQWMAAREQPPHLTALVSTAGAGRWMEELPYNNGIFAA
                     YWMFWLNLVGGRTLQQPIHGTVSSPDWPRIFTHMPLRDTDLALGRTNTAWRMWLEHNT
                     FDDYWRQLSLTGHFDKIDLPVLHITGWFDGDQWGELFYWHGMLKDSPAADRQWLLSGP
                     WVHAGTRTPKQHLGGRDFTAAAVSDMNAIHLRFFDRWLKGIENGQEQDKRVRIFAMGD
                     NQWRDEDQWPPAGTVETPFYLHSAGAANTLAGNGSISQKAPESDEPSDSYIYNPDNPT
                     PSVQDIASLPFGDVPLDNRWRLRRDDVLVYTSEPLMADLEITGHPFIVLHAESDCPDT
                     DWHVALCDVHPDGRSEQLTDGAVRAAYRGGIEAQPTAIEPGRVYEYRIELMATSNVWK
                     AGHRLRVTVASANWPGSARNPNTNAPTGDDDEVRIATNTVHHTKAYPSRLLAPV"
     gene            1874..>2265
                     /locus_tag="VFV93_17400"
     CDS             1874..>2265
                     /locus_tag="VFV93_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167186.1"
                     /translation="MSSEQPVIADLHCHIFPVEAAIRAGTSVPIEERDGSFWVGGVDL
                     DAGLLDLGIQIEDMQRQGVARRALAIPPFTLRYDLPADEGTRWARAINEGIAEAIAPH
                     GEAFVGFATVPLQDVAAAVAELDYAVGEL"
BASE COUNT          346 a          677 c          822 g          420 t
ORIGIN      
        1 gaccggcgcg agtagtcgcg acgggtacgc cttcgtatgg tggacggtgt tggtcgcgat
       61 ccggacctcg tcgtcgtcgc cggttggggc gttggtgttg gggttacggg ccgagccggg
      121 ccagttggcc gaggcgacgg tcacgcgcag acggtggccg gccttccaga cgttcgaggt
      181 cgccatcagc tcgatgcggt actcgtagac acgacccggt tcgatcgccg ttggctgggc
      241 ttcgatcccc ccgcggtaag cggcgcggac ggcgccgtcg gtgagctgct cggagcggcc
      301 atccgggtgg acatcgcaga gggcgacgtg ccagtcggtg tcggggcagt ctgattcggc
      361 atgcaggacg atgaacgggt ggccggtgat ctccaggtcg gccatcagcg gctcggaggt
      421 gtagacgagg acatcgtcgc gccgtagccg ccagcggttg tcgagcggga catcgccgaa
      481 cggcaggctg gcgatgtcct ggacgctggg ggttgggtta tcggggttgt agatgtacga
      541 gtcggacggc tcgtcgcttt ccggcgcttt ctggctgatg ctgccgttgc cggccagcgt
      601 gttggcggcg ccggcagagt gcaggtagaa cggcgtctcg actgtcccgg ctgggggcca
      661 ctggtcctcg tcgcgccact ggttgtcgcc catcgcgaag atgcggacgc gcttgtcctg
      721 ttcctggccg ttttcgatcc ctttcagcca gcggtcgaag aagcgcaggt ggatcgcgtt
      781 catgtcgctg acggcggcgg ccgtgaagtc gcgcccgccc aggtgttgct tgggggtgcg
      841 ggttccggcg tggacccatg ggccggagag gagccactgg cggtcggccg ctggtgaatc
      901 cttcagcatc ccgtgccagt agaacagctc gccccactgg tcgccgtcga accagccggt
      961 gatgtggagg acgggcaggt cgatcttgtc gaagtggccg gtcagcgaga gctgccgcca
     1021 gtagtcgtcg aacgtattgt gctccagcca catgcgccag gcggtgttgg tccggccgag
     1081 ggcgaggtcg gtgtcgcgca gcggcatgtg ggtgaagatc ctcggccagt cggggctgga
     1141 aaccgtgccg tggatcggct gctgcaaggt ccgaccgccg accaggttga gccagaacat
     1201 ccagtaggcg gcgaagatgc cgttgttgta gggcagctcc tccatccagc ggccggcgcc
     1261 ggccgtgctg acgagagccg tcagatgcgg cggctgctcg cgggccgcca tccactggac
     1321 gaagccgccg taggagccgc ccatcatgcc gaccttgccg tcgcaccact cctgttccgc
     1381 gatccactcg atcgtgtcgt ggccgtccgg tccctcattg acgaacggct cccattcgcc
     1441 gccggaatca cagcgcccac gaacgtcctg cgagacgaag gcgtagccac gctgggcaaa
     1501 gtagacggcg atgttgaccc agagcgcggc cgtgttgtcg tacggcgtcc gctggaggat
     1561 ggccggccat ggcccgtcac ccgttggcgg caggtagaca tcggccgata gctcgacccc
     1621 atcacgcatc gtcacccgct ggtccagcag cagccgtggc gcggtccgtt cggtggtctc
     1681 cggcatctca gatttcctcc ccggtcagca ttcagcaacc gcgcgagggt atggggatcg
     1741 gggcagggtg tcaacaatcg catcaatgta ggggcgaatc accgattcgc ccctacattg
     1801 atgcgattga tcggtgttct cgacagtttc cgcgcatcac gcgacaatca accattcagg
     1861 acaggagggt gtcatgtcca gcgaacagcc ggttatcgcg gatctgcatt gtcatatctt
     1921 cccggttgag gcggcgatcc gggccgggac atcggtgccg atcgaggagc gggatggcag
     1981 cttttgggta ggtggcgttg atctggacgc cgggttgctt gatctgggca ttcaaatcga
     2041 ggacatgcag cggcaagggg tggcgcggcg ggcactggcg atcccgccgt tcacgttgcg
     2101 ctacgacctg ccggcggacg agggaacgcg ctgggcgcgg gcgatcaacg aggggatcgc
     2161 cgaggcaatc gccccgcatg gtgaggcgtt cgttggcttc gcgacggtgc cattgcagga
     2221 tgtcgcggcg gccgtcgcag aactcgacta cgctgttggc gagct
//
LOCUS       DASUGJ010000771         4684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1238230, whole genome shotgun sequence.
ACCESSION   DASUGJ010000771 DASUGJ010000000
VERSION     DASUGJ010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238230"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..901)
                     /locus_tag="VFV93_17405"
     CDS             complement(158..901)
                     /locus_tag="VFV93_17405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5167187.1"
                     /translation="MLFNDHYGMVDLDSVQTAGDASVETGRGRAGEESIRVLVVDDNF
                     VVRRGLQSSLELDSSIVVVGEGANGDEAIELARELVPDVILMDIRMPLRDGIAATEII
                     SDDAPSSKVLMLTWSEDPEHLRSAVLAGAKGYLVHGRFSPERLAEAVRVVHDGGALIT
                     PMLAPALLEFVRASAQGQRGRSAAGSILTPREIEVLEYIVAGKSNREIAEALYIEEKT
                     VKNHVSNIYSKLHLKNRFEAIARGRDHLR"
     gene            complement(888..2186)
                     /locus_tag="VFV93_17410"
     CDS             complement(888..2186)
                     /locus_tag="VFV93_17410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5167188.1"
                     /translation="MASRGTAAESGDINQSPSIPARAGSTLRVWLAAQLDQLARLQRV
                     LRRRARRLHPLTAYRIIALAIAILITASNLHHESTVISDAQMLLLAAFHVGLTLALAP
                     RMERRSDALNLVVIAIDIVACVGLMSLAHGWRGPLWLYSLSAVFWPAWIFSMRGALLS
                     VTAFDALVLLMNRDQIQDAVSDGFGGDVVARLLMIYLIAGAISLTSQAFATVRRMAAE
                     SERNRIARDLHDGVGKTMGGISMEALTLAHWIDRDPAEASRRARYVARISEKAAIEVR
                     DVIRGLRRTEATELLFPAVREIVGDWALAHPSVDTQLRLNGNDAPVPVLIHGEIVRML
                     SELLRNVDRHAAAGHVWVRVTLSSAGVTLAVRDDGVGFDALSLDPWSDEGHFGLLGAR
                     ERALMLAGHVRVVSKPGSGAEITIDLPLSAREDRVVHVIQ"
     gene            complement(2610..4136)
                     /locus_tag="VFV93_17415"
     CDS             complement(2610..4136)
                     /locus_tag="VFV93_17415"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX5167189.1"
                     /translation="MPQIFEQVNPDLSLAIPQLIVLGLALLLLVADIAIPRARHGILT
                     WITLAGFAAALVATLYYVYDNTNDSTFTTPGYGDMFLTDGFSIFISLVVLTGGILSVM
                     ISGSYLQGRSEDPGSMPLAEYYILLSLSVLGTMITGSAGDLLMVFLGIEMSSLAVYAL
                     TAFARHRTTSLEGALKYFLLGLFASAFLVYGMAWIYGATGTTNLDGIAVALSGQVTDG
                     DIEASLLLALLLLVVGFGFKVAAVPFHMWTPDAYQGAPTPVSAYMSVIPKVAGFAAMI
                     RVLVQGLAPLSDEWITIIAILAVLTMAFGNIVAVSQRDVKRMLAYSSIGHTGYILAGV
                     AAFSTITQDGENAISSVLYYLFAYAFMNIGAFAVVTWLQHQGYGTTIDDFNGLGTRSP
                     LAALAMTIFMLSLMGLPPLVGFYGKYYVILATIEADMIWLAIAIVLASAVSAFYYLRV
                     VAAMYFSEAPEGAEEPSRTPMLGFGVMTMAVGTIAFGLFSGQIADIANRWVQAFSISM
                     "
     gene            complement(4219..>4684)
                     /locus_tag="VFV93_17420"
     CDS             complement(4219..>4684)
                     /locus_tag="VFV93_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167190.1"
                     /translation="GEFLSVLGAFREYRWAAIISFSVVIFSAWYMLWLFQRVVFGRAP
                     GELPDPGDNSLTDEERAMLAAHGDHGHGHAVPAVSGGSGLDEDNHGHAAHADVDKDGR
                     ALHMPDLNTNELWTLVPLLVLTIALGVYPGPVMDYVKVTFEAILVPFGGTGV"
BASE COUNT          921 a         1524 c         1484 g          755 t
ORIGIN      
        1 acgtctcgaa catctactcc aaactgcacc tcaagaaccg ctacgaagcg atcacgcggg
       61 gccgcgatca tctgcgctga cgatgaaacc gatgctactt cccagatgaa cggcgtcgaa
      121 ccggtcccga cccctcgccc ctgcgggttg cgtgcggcta gcgcagatga tcgcggcccc
      181 gcgcgatcgc ttcgaagcgg ttcttgaggt gcagcttcga gtaaatgttc gagacgtggt
      241 tcttgaccgt cttctcttcg atgtagagtg cctccgcgat ctcccggttc gacttgccgg
      301 cgacgatgta ttcgagcacc tcgatctcgc gaggggtcag gatcgagccg gcggccgagc
      361 gtccacgttg cccctgggcg ctggctcgga cgaactcgag tagcgcagga gccagcatcg
      421 gcgtgatcag cgcgccgcca tcgtggacga cgcggacggc ctcggccagc cgctccggcg
      481 agaagcgacc gtggacgagg tagcccttgg cgccggccag gacggcactg cgcaggtgct
      541 cggggtcttc cgaccaggtc agcatcaaca cctttgagct gggcgcgtcg tcgctgatga
      601 tctcggtcgc ggcgatcccg tcgcgcagcg gcatccggat gtccatcagg atcacgtcgg
      661 gaaccagctc gcgggccagc tcgatcgcct cgtcgccgtt ggccccctcg ccgacgacga
      721 cgattgatga gtccagctcg aggctggatt gcagcccgcg ccggacaacg aagttgtcgt
      781 cgacgaccag cacgcggatc gactcctcgc cggcccgccc gcggccggtc tcgaccgagg
      841 cgtctccagc ggtctgaacg ctgtcgagat cgaccatgcc gtagtggtca ttgaataaca
      901 tggacaaccc gatcttcgcg tgcgctcaat ggcaggtcga tcgtgatctc cgcgccggaa
      961 ccgggcttcg agacgacccg cacgtgacca gccagcatca gcgcacgctc gcgggcgccg
     1021 agcagcccga agtgcccctc gtccgaccac ggatcgagcg acagcgcgtc gaagccgacc
     1081 ccgtcgtcgc ggaccgcaag cgtcacgccg gccgacgaca gcgtgacccg gacccagacg
     1141 tgccccgcag cggcatgccg atcgacgttg cgcagcagct cggagagcat ccgcacgatc
     1201 tcgccgtgaa tgagaaccgg aacgggcgcg tcgttgccgt tgagacggag ctgggtatcg
     1261 actgacgggt gcgccagcgc ccagtcgccg acgatctccc tgacggccgg gaacagcagc
     1321 tctgtcgctt cggttcggcg caacccgcgg atgacatcgc ggacctcgat ggccgccttc
     1381 tcggagatgc gcgcaacata gcgcgcgcga cggctggcct ccgccgggtc gcggtcgatc
     1441 cagtgcgcca gcgtcagcgc ctccatgctg atgccgccca tcgtcttgcc gacgccatcg
     1501 tgcaggtccc gagcgatgcg gttgcgctca ctctcggccg ccatccgccg gactgtcgcg
     1561 aacgcctgcg aggtcagcga gatcgccccg gcaatcaggt agatcatcag cagccgggcg
     1621 acgacgtcgc cgccgaagcc gtcggacaca gcatcctgga tctgatcccg gttcatcagc
     1681 aggaccagcg cgtcgaacgc cgtcacgctc agcagcgcgc ctcgcatcga gaatatccag
     1741 gccggccaga agaccgccga caggctgtaa agccagagcg gcccgcgcca gccgtgtgcg
     1801 agcgacatca accccacgca ggccacgatg tcgatcgcga tgacgaccag gttgagcgcg
     1861 tcggaccggc gttccatccg tggcgccagc gccagcgtta gcccaacgtg aaacgcagcc
     1921 agcagcagca tctgtgcgtc ggagatgacg gtactctcgt ggtgcaggtt cgaagccgtg
     1981 atgagaatcg cgatcgccag cgcgatgatg cggtaggcgg tcagtggatg cagtcgtctg
     2041 gcgcgacggc ggagcacacg ctgcaggcgg gcaagctggt cgagctgagc cgcgagccag
     2101 acacgcagcg tcgagcccgc gcgggcgggt atcgagggag actgattgat gtcgccgctc
     2161 tcggcagccg ttcctcgcga ggccacgatc tgtacccctg ctcaggcacg atccaacgct
     2221 gctctaccag tctagcattc cgctggaaca gccggtccgg cgtgatcatc tgggataggg
     2281 ggcccagatg aaatctgggt ccatggtccg atgccgccat tttgacgctg attatcctaa
     2341 ggacagatcg ctgttgtgac aggacacacc agtggtcagt ctcactctct cctcccctct
     2401 tttggccgca ggaaggactg gagcgaaacc cgctccagtc cttcttgcgt ctgccgtcag
     2461 acaggaatct tgacggacac aatccacaat caatccggta cgggcgatat cgcaatatcg
     2521 ccctttcaaa cgcccagcgc tcggggaagg gcgatattgc gatatcgccc gtaccggatt
     2581 gtgacattag attgtgtggc agcgctcgcc tacatcgaga tcgagaacgc ctggacccag
     2641 cgattcgcga tgtcggcgat ctggccggag aagaggccga aggcgattgt gccaacggcc
     2701 atcgtcatca ctccgaagcc gagcatcggc gttcgcgacg gctcttccgc gccttcgggc
     2761 gcttccgaga agtacatcgc cgccacgacc cgcaggtaat agaaggccga gactgccgag
     2821 gccagcacga tcgcgatcgc cagccagatc atgtcggctt cgatcgtcgc caggatgacg
     2881 tagtacttcc cgtagaagcc caccagcggt gggagtccca tcagcgagag catgaagatc
     2941 gtcatcgcca gcgcagccag cggcgagcgg gtgccgaggc cgttgaaatc gtcgatggtc
     3001 gtgccgtatc cctggtgttg taaccaggtc acgaccgcga atgcgccgat gttcatgaat
     3061 gcgtaggcaa acagatagta cagcactgac gaaatcgcgt tctcgccgtc ctgggtgatc
     3121 gtcgagaagg ccgccacacc ggccaggatg tagccggtat ggccgatcga tgagtaggcc
     3181 agcatacgtt tcacatcgcg ttgggaaact gcgacgatat tgccgaacgc cattgtcagg
     3241 accgccagaa tcgcgatgat cgtgatccat tcatcgctga gcggcgccaa cccctggacg
     3301 agcacccgga tcatcgccgc gaagcccgcc accttcggga tgaccgacat gtaggccgag
     3361 acgggtgtcg gtgcgccttg gtacgcgtcc ggcgtccaca tgtggaacgg cacggctgcg
     3421 accttgaagc cgaagcctac gaccagcagc agcagcgcca gcaagagcga agcctcaatg
     3481 tcgccatcgg tgacctgacc ggagagggca accgcgatcc catccagatt cgtcgtcccg
     3541 gtcgcgccgt agatccaggc catgccgtag acgaggaagg ccgaggcaaa cagacccagc
     3601 aggaagtact tcaatgcgcc ttcgagcgag gtcgtccggt ggcgcgcgaa ggctgtcagc
     3661 gcgtagacgg ccagcgagga catctcgata ccgaggaaga ccatcagcag gtcgccggcc
     3721 gagccggtga tcatcgtccc gaggaccgac agcgagagca ggatgtagta ctccgccagc
     3781 ggcatcgagc cgggatcttc cgaacggccc tgcagatagc taccggagat catgaccgag
     3841 aggatgccgc cggtcaggac gacgagactg atgaagatgc tgaagccgtc agtcagaaac
     3901 atgtcgccgt agccgggcgt cgtgaaggtc gagtcgttgg tgttgtcgta gacgtagtag
     3961 agggtcgcca ccagcgcggc agcgaagccg gccagcgtga tccacgtcag aataccgtgg
     4021 cgcgcgcgcg gtatcgcgat atcggccacc agcagcagca gtgccagccc gagcacgatc
     4081 agctgcggga tcgcgagtga caggtctgga tttacctgct caaagatctg cggcaccgaa
     4141 cttccccccg ttagctatca gccagacagg ttgtcagcct gtcagcgttg tgccaccgtc
     4201 ggtggcgcgt caacgtccct aaacgccggt cccgccgaac ggaacgagaa tcgcctcgaa
     4261 cgtcaccttc acgtagtcca tgaccggccc ggggtagaca cccagcgcga tcgtcaggac
     4321 cagcagcggc accagcgtcc acagctcatt tgtgttcaga tcgggcatgt gcaacgcccg
     4381 gccgtcctta tcaacatcag cgtgcgccgc gtgcccgtga ttgtcctcgt cgaggccgga
     4441 gccgccagag acggccggaa cggcatggcc gtgaccgtgg tcgccatggg ccgccagcat
     4501 cgcccgctcc tcgtcagtga gcgagttgtc acccggatca ggtagctcgc cgggtgcgcg
     4561 accgaagacg acgcgctgga agagccagag catgtaccac gccgagaaga tcacgacgct
     4621 gaaggagatg atcgctgccc agcgatactc ccggaacgcg ccgaggaccg acagaaactc
     4681 accg
//
LOCUS       DASUGJ010000772         7036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_683403, whole genome shotgun sequence.
ACCESSION   DASUGJ010000772 DASUGJ010000000
VERSION     DASUGJ010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683403"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..947)
                     /locus_tag="VFV93_17425"
     CDS             complement(87..947)
                     /locus_tag="VFV93_17425"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5167191.1"
                     /translation="MSASETETRGKAKRQLEEMARDLAAEGRWAEAIEVNQQFIERSP
                     KDVNAFNRLGKAYFELHRYRSAYEAYSTAATLDPANVIAQRNLSRIEPLKAADEDGDI
                     EPLHYVIRPGIFIEEVGKTYVDDLVHVAGQEVLTELSSGIQLTLTVEGDDVSVVDQAG
                     RYIGQVEPRLAKRLIELLEIGNRYEVYVTSNSGQSVRVIIREAYKHPDMGNRLSFPRQ
                     GKVAIPRAYLRDTRLFRDEPDMLLGDDEDEDLSDEEAEDYEASDGDEDESEYVEESGG
                     PADEDEESAI"
     gene            complement(1000..2367)
                     /gene="miaB"
                     /locus_tag="VFV93_17430"
     CDS             complement(1000..2367)
                     /gene="miaB"
                     /locus_tag="VFV93_17430"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX5167192.1"
                     /translation="MEPLHLIPRVTRAQAGSSPAGDEKRYCIWTIGCQMNEAESAKVE
                     AMLSQVGYRRTETEDDADVIVLNSCVVRQAAEDKVVGKIGSLARVKRSKPDVRIALTG
                     CMVTGQETKLRERFPYVDLFFEPSEFDRLVEIVPELAEADAELAELPHYYQPVATNPG
                     VSAFVPIIYGCNFLCSYCIVPYRRGKEISRPFDEIVGEVERLASQGVREVTLLGQTVN
                     AYGHDLPDGRDLADLLTAVDAVPGIDRLRFLTSHPKYFSDKLIETMAALSSVCEHVNL
                     PVQAGDDEVLRRMRRTYTADHYRDRIAKIRATIPGVTVSTDIIVGFPGETDEQFDNTM
                     RLLEEIEFDKVHVAMYSPRPGTLSARWEDDIPHDVKHARHTRVEKLMERVSAAHYAAM
                     VGETREILVDGFAKGRWRGRTRGNQLVFFEATGDWLGQMVDVRITTASTWYLLGEPVS
                     VLAIA"
     gene            2823..4151
                     /locus_tag="VFV93_17435"
     CDS             2823..4151
                     /locus_tag="VFV93_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167193.1"
                     /translation="MVLVRKTELACRNSWDQDLWELADQPMDIPTSAHVPLPLSGSEP
                     QDFPIVRAEVADDDAARAGEMFTDRITSISMPTRSQLRPGRQDADQIEATDGTDTEAD
                     LLNARSSVREARKRRQEQRHQERRKHQETVLQRADDLLDTTEPANENDRDLARIRAVD
                     GPMTTGRERKADRQPETREQHTGGDRPVQPRRPVGGEQTPVRREPVPAIEFSAVDPVF
                     ETGPTAPPAPQPDSKAAPAKKVNGAGSSAFKRGETEPLPSPAEILDAARQRNAQLTGE
                     AEAPAKREASARVSSARPAEQRPARSMPGDLPPAVESRPTRWFTGAGHLPGERKNEIA
                     APFPVDPIDLASDLKTVRRCCATCRDFKQIGDGRTGWCNNPYAFKERRMVQSDELACR
                     SSLGVWWLPHDDLWLEYADTTHHGRPTPLLDDLVGAVADERQRMGQRSSR"
     gene            4229..5134
                     /locus_tag="VFV93_17440"
     CDS             4229..5134
                     /locus_tag="VFV93_17440"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX5167194.1"
                     /translation="MTEFGQTLRQAREAKSVSLAQAERATRISRTYLGALEQHEFSEL
                     PAATYARGIVRNYAAYLGLEPAEMLAAYEALTDSKPASFEVMPATKPLDMSTHWAPNF
                     AIIAFMIVMSLVVFTWMYSAYFREADSLATGTVGVATVTPVSESILAAIATQPTVATQ
                     GGGVATVTPSPTTPATATVQPTATTAVVPTETPVPSTDAAAVEDTEEDVVVEPTEAVV
                     DTSDDAATTGAHTFVIWVTDEVWVGVSVNGESVADDVQPAGAELIFQGDSLSVTSGNS
                     AYVVVYVDGVEYALGETWDATFIYP"
     gene            5163..5579
                     /locus_tag="VFV93_17445"
     CDS             5163..5579
                     /locus_tag="VFV93_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167195.1"
                     /translation="MSGPAVFGKNPYVAKSDVTGRVVAVLRGVTDQRGLYLTAFRSRA
                     VPAGEIHELMITDEGCDVETTVNRVALIAFFEVSQGGVILLDDTVTVGDLSVGTVAGF
                     DETHMPNHQNICLRGELRDGEGLGIQVGDPVRIARD"
     gene            complement(5560..6471)
                     /locus_tag="VFV93_17450"
     CDS             complement(5560..6471)
                     /locus_tag="VFV93_17450"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5167196.1"
                     /translation="MPPQRVLAIINPATGRYPVGSIEADLRYEAYRRNIELELAQTTR
                     PGHAIELAAAAVDDVDTIIAVGGDGTVSDVVTGSIGTSVTVGIVPAGSTNIIARELSI
                     PKDINLAAAIALGDGHDAMFDVARAGETTFMHMAGAGFDAAIMGDTPSRWKRRVGWLA
                     YLPAAARHLNAPRFTARLTIDGTPQETSARLVLCAIGGAIVHPRFRVGEQIDRTDGIL
                     DILIYDPPGLGGTLSSLAWIVAGHPGRSRWLTQRRGRSVLLDADQKVPFEVDGDFVGY
                     VPVEIAMLEHQARIRVPVSARVSPEQS"
     gene            complement(6700..7023)
                     /locus_tag="VFV93_17455"
     CDS             complement(6700..7023)
                     /locus_tag="VFV93_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167197.1"
                     /translation="MAIRPPRATRPPRRTRLVRDLVIIALVSLAVLLFGLRCLLAILG
                     IETWTATWKLVDVPTGLLVAPLEKIDALTQTPIGDLTLATIIVSAVCFLAALVVLGSL
                     ANQRD"
BASE COUNT         1256 a         2219 c         2330 g         1231 t
ORIGIN      
        1 aggacctctc ctcattatcc gcgcgtggcg tttagtcggg gaggtccttc gctgccgggt
       61 gccctctggg cggtggtcga caaccgttag atcgcgcttt cttcgtcctc gtcggccggg
      121 ccgccggact cttcgacgta ctccgactcg tcctcgtccc cgtcgctggc ttcgtagtcc
      181 tcggcttctt cgtcgctgag gtcttcgtcc tcgtcgtctc ccagtagcat gtccggctcg
      241 tcgcggaaga ggcgcgtgtc acgcaggtag gcgcgcggga tcgcgacctt gccctgacgc
      301 gggaaggata gccggttgcc catgtcgggg tgcttgtagg cttcgcggat gatgacgcgg
      361 acgctctgtc cgctattgga cgtgacgtag acttcgtagc ggttgccgat ctccagcaac
      421 tcgatcaggc gcttggccag tcgcggctcg acctggccga tatagcgccc ggcctggtcg
      481 acgacgctga cgtcgtcgcc ttcgaccgtc agggtcaact ggatgccgct cgacagttcg
      541 gtcagcacct cctggccggc gacatggacg aggtcgtcaa cataggtctt gccgacttct
      601 tcgatgaaga tgcccggccg gatgacgtag tgcagcggct cgatgtcgcc gtcctcgtcg
      661 gcggccttaa gcggctcgat ccggctcagg ttgcgctggg cgatgacgtt ggccgggtcc
      721 aacgtcgcgg ctgttgaata cgcttcatag gccgagcgat agcgatgcag ctcgaaatag
      781 gccttaccga gccggttgaa ggcgttgaca tccttcggcg accgctcgat gaactgctgg
      841 ttgacctcga tcgcctcggc ccagcggcct tcagctgcca gatcgcgcgc catctcttcg
      901 agttggcgct tggctttgcc gcgtgtttcg gtttcggatg ctgacacgtt ctaccctccc
      961 tgatggttgg ttgctctccg cgctcaatga tgtcggtggt tatgcgatcg ccaggactga
     1021 gaccggctcg ccgagcagat accaggtcga ggcagttgtg atgcgaacgt cgaccatctg
     1081 gccgagccaa tcgccagtcg cctcgaagaa gacgagctgg ttgccacgcg ttctgccccg
     1141 ccagcgtccc ttcgcgaagc cgtcgacgag aatctcgcgc gtctcgccga ccatcgccgc
     1201 gtagtgagcc gcgctgaccc gctccatcag cttctcgacc cgcgtgtgcc gggcgtgctt
     1261 gacgtcgtga gggatatcgt cttcccagcg cgccgagagc gtgccgggcc gtggcgagta
     1321 catcgcgacg tggaccttgt cgaactcgat ctcctccagc agccgcatcg tgttgtcgaa
     1381 ttgttcgtcg gtctcgcctg ggaagccgac gatgatatcg gtcgagacgg tgacgcccgg
     1441 aatcgtggcg cggatcttcg cgatccgatc gcggtagtga tcggccgtgt aggtccggcg
     1501 catgcgccgc agcacttcat catcaccggc ctgaaccggc aggttgacgt gctcgcatac
     1561 cgacgacagc gccgccatcg tctcaatcag cttgtcggag aagtacttgg gatgcgatgt
     1621 caggaagcgc agacggtcga tcccgggaac ggcgtcgact gccgtcagca gatcggccag
     1681 atcgcggcca tccggcaggt cgtggccgta ggcgttgacc gtctgtccaa gcagcgtcac
     1741 ctcgcgaact ccctggctgg cgagacgctc gacctcgccg acgatctcgt cgaacgggcg
     1801 gctgatctcc ttgccacgcc ggtagggcac gatgcagtac gagcagagga aattgcagcc
     1861 gtagatgatc gggacgaagg cggagacgcc cgggttggtt gccaccggct gatagtagtg
     1921 cggcagctct gccagttccg cgtcggcctc ggccagctca ggaacgatct cgaccagccg
     1981 gtcgaactcg gacggctcga agaagagatc gacgtacggg aagcgctcgc gcagcttcgt
     2041 ctcctggccg gtgaccatgc agccggtcag cgcgatccgg acgtccggct tcgagcgctt
     2101 gacgcgggcc aacgagccaa tcttgccgac aaccttgtcc tcggctgcct ggcgaacgac
     2161 gcatgagttc aggacgatga catcggcgtc gtcctcggtc tcggtgcgcc ggtagccaac
     2221 ctggctgagc atcgcctcga ccttggccga ctcggcctcg ttcatctgac agccgatcgt
     2281 ccagatacag tagcgcttct cgtcgccggc gggagacgag ccggcctgcg cgcgcgtcac
     2341 tcgcgggatg agatgcagtg gctccaaagc aacctccagg aatacagata tggcgccgct
     2401 ggcgcccaac tgacgaagta tacgcgtcac gcacgtacgg cacgagttgc gacggccgga
     2461 cggccatcag tgaggctggc caatggtgac cgtcagtccc cggttgcggc ttgacagaac
     2521 ccgtagaaca ggcgttcgga tggaaaaatt gctgctgaat tggggtgtac gtacatctgt
     2581 tggcgactcg gggccatgct acaatcgcaa gcgacgcaac ggacggtgac caggtggaac
     2641 agggcaccag cggcaaggtc ggtgggtcct tgccgcgtac gaacgcgcct tatttcgctc
     2701 tccggggatg caggggtggt ctgatggtga agcgaaagtg cggaacgtgc cgtcatttca
     2761 aggacggtgg gatcgctgga tccgggtggt gccagcatca tgctcgcaag gacatccagc
     2821 acatggtcct ggttcgcaaa accgaattgg catgtcgaaa tagttgggat caggatctct
     2881 gggaactcgc cgatcaaccg atggacattc ccacatcggc gcacgtaccg ctgccactca
     2941 gtgggtccga gccgcaggat tttccgatcg tccgggccga ggtcgccgac gacgacgcgg
     3001 ctcgtgccgg ggagatgttc accgatcgca ttacctccat cagcatgccg acccggtcgc
     3061 aactacgacc gggccggcag gacgccgatc agatcgaagc gactgacggc actgacactg
     3121 aggcggatct gctcaacgcc cgatcgagcg tccgcgaggc gcgcaaacgg cggcaggagc
     3181 agcgccacca ggaacggcgc aagcatcagg agacggtctt gcagcgggcc gacgacctgc
     3241 tcgacacgac cgagccggcc aacgagaatg accgcgatct cgcccgtatc cgggccgttg
     3301 acgggccgat gacgaccggt cgtgagcgga aagcggatcg acagccggag acgagagagc
     3361 agcacaccgg tggcgatcga ccggttcagc cgcgccggcc ggtggggggc gaacagactc
     3421 ccgtccgtcg ggaaccggtc ccggcgattg agttcagcgc cgtcgatccg gtcttcgaga
     3481 ccggccccac agcaccgccc gcgccgcaac cggattccaa agccgctccg gcgaagaagg
     3541 tgaacggggc gggcagcagc gccttcaaac gcggcgagac agaaccgctg ccgtcaccgg
     3601 ccgagatcct tgacgcagcc cggcagcgca acgcgcagct gaccggcgag gccgaagcgc
     3661 ccgcgaaacg tgaggcgagc gccagggtca gcagcgcgcg tccggccgag cagcggccag
     3721 cccggagcat gccgggtgat ctgccgcccg ccgtcgagtc gcggccgacg cgctggttca
     3781 ccggcgccgg gcacctgccg ggtgagcgga agaacgagat cgccgcgccg tttccggtcg
     3841 atccgatcga cctcgcgagc gacctgaaga cggtgcgccg ctgttgcgcg acctgccgcg
     3901 atttcaagca gattggcgac ggtcgaacag gctggtgcaa caacccgtat gccttcaaag
     3961 agagacgtat ggtacagtcg gacgaactag cgtgccgttc aagtctcggc gtctggtggt
     4021 tgccacatga tgatctgtgg cttgagtacg ccgacacaac tcatcatgga cgtccgacgc
     4081 cgttgctcga tgatctggtc ggcgcggtgg cggacgaacg acaacggatg gggcagcgat
     4141 cctcacggtg aggcgcgggt agaggggttc gttctggcaa tcggtaatgg gggccggcca
     4201 gggcaaccag ggggcaaccg ttggcactat gacagagttt gggcaaacgc tccggcaagc
     4261 ccgtgaagcg aagagtgtct cgctggcgca ggccgagcgg gcgacgcgca tcagcagaac
     4321 ctaccttggt gcacttgagc aacacgagtt ctcggaactc cccgcagcga cctacgctcg
     4381 cgggatcgtc cgcaactacg ccgcctacct tgggctggag ccggccgaga tgctggctgc
     4441 ctacgaggca ctgaccgatt cgaagccggc ctcgttcgag gtcatgccag ctaccaaacc
     4501 gctcgacatg tcgacccact gggcgccgaa cttcgccatc atcgcgttca tgatcgtcat
     4561 gtcgctggtc gtcttcacct ggatgtattc agcctacttc cgcgaggccg actcgctcgc
     4621 gaccgggaca gttggagtcg cgaccgtgac gcccgtctcc gagtcgattc tggcggcgat
     4681 tgcgacccag ccaacggtgg cgacccaggg cggaggcgtc gcaacggtga cgccgagccc
     4741 gacgacgccc gcgacggcca ctgtccagcc gacggcgacg acggcggtcg taccgaccga
     4801 gacgcccgtg ccttcaaccg atgcagctgc cgtcgaggat accgaggagg atgtcgtggt
     4861 agagccgacc gaagcggtcg ttgacacttc ggacgacgcc gccacgaccg gcgcgcacac
     4921 cttcgtcatc tgggtgaccg atgaggtctg ggtcggtgtg tctgtcaatg gggaatcggt
     4981 cgcggacgat gtccagccgg cgggcgccga actgatcttc cagggcgact ccctaagtgt
     5041 cacgtcgggc aactcagcct atgtcgtcgt ctacgtcgac ggcgtcgagt acgcgctcgg
     5101 cgagacctgg gatgctacgt tcatctatcc atagctatgt cggccacggg agcgtgcgcg
     5161 caatgtcggg accagccgtc tttgggaaga atccgtatgt cgccaagtcg gacgtgacag
     5221 gacgggtcgt cgccgtcctg cgcggtgtca ccgaccagcg cgggctgtat ctgacggcct
     5281 tccggtcgcg ggctgtcccg gccggtgaga tccacgagct gatgatcacc gacgagggct
     5341 gtgacgtcga aacgacggtc aaccgcgtcg cgttgattgc cttcttcgaa gtcagtcaag
     5401 ggggcgttat cctgctggac gataccgtca cggttggcga cctgtcggtt gggacggtgg
     5461 cgggtttcga cgagacccac atgccgaatc accagaacat ctgcctacgc ggggaactgc
     5521 gcgatggcga ggggctcggc atccaggtcg gcgatcccgt caggattgct cgggactgac
     5581 gcgggccgac accggtaccc gaatgcgtgc ctgatgctcc aacatcgcga tctcgaccgg
     5641 cacatagccg acaaaatccc catcgacctc aaatggcacc ttctgatcgg cgtcgagcag
     5701 gacgctgcgg ccccgccgtt gcgtgagcca gcgcgagcgt cccggatggc cggccacgat
     5761 ccaagccagg cttgagagtg ttccacccag tccgggtgga tcgtaaatca ggatgtcgag
     5821 gatgccgtca gtccgatcga tctgctcgcc gacgcgaaag cgcgggtgga cgatcgcccc
     5881 gccgatcgcg cagagcacca gccgtgccga cgtctcctgc ggcgtgccgt cgatcgtcag
     5941 cctggcagtg aaacgcggcg cattcaggtg gcgggcggcg gccggcaggt aggccagcca
     6001 gccgacgcgc cgcttccaac gcgacggtgt gtctcccatg atggcggcat cgaatccggc
     6061 gccggccatg tgcatgaagg tcgtctcgcc ggcccgcgcg acgtcgaaca tcgcgtcgtg
     6121 gccgtcgccg agcgcgatcg cggcggccag gttaatgtcc ttgggaatgc tcagctcgcg
     6181 ggcgatgatg ttggtcgaac cagccgggac gatgccgacc gtgacgctgg tcccgattga
     6241 cccggtcacg acatcggaga cggtgccatc gccgccgacg gcgatgatcg tgtcgacatc
     6301 gtcaaccgcg gcggctgcca gttcgatggc gtgaccgggc cgcgtcgtct gcgccagttc
     6361 aagctcgatg ttgcgccggt aggcttcgta gcgcaggtca gcctcgatcg atccgacagg
     6421 gtatctgccg gtggccgggt tgatgatggc gagtacacgt tgcggcggca tccggtcagc
     6481 ggctcaggcg tcctcgaaga aatcttcagg accgaagacg cgttcagtga tccagttggc
     6541 gagccaggtc gccgcagcgg cgaggaacaa acccaggatt ccacggacca cgtttcgcat
     6601 catcgctcgt actccatctg cattccaacc aacgacgccc ccagttgagc gactggggac
     6661 attggcagtc taccatcggt ggcgggacgg attgcagcct cagtcgcgct ggttcgccag
     6721 tgagccgagg actaccagtg ccgccagaaa gcagacggca gagacaatga tcgtcgccag
     6781 cgtcagatcg ccgatcggcg tttgtgtcag cgcatcgatt ttctcaagcg gcgcaaccag
     6841 caagccggtc ggcacatcaa ccagcttcca ggttgccgtc caggtctcga ttcccaggat
     6901 cgccagcagg cagcgtaggc cgaagagcag gacagccagc gacacgagcg cgatgatgac
     6961 cagatcgcgc accagccggg tgcggcgtgg cggtcgggtc gcgcgtggtg gacgaatcgc
     7021 catcgatcag gaggtc
//
LOCUS       DASUGJ010000773         9085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_545695, whole genome shotgun sequence.
ACCESSION   DASUGJ010000773 DASUGJ010000000
VERSION     DASUGJ010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545695"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFV93_17460"
     CDS             <1..278
                     /locus_tag="VFV93_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167198.1"
                     /translation="RLLPFHQYARDGYTHSGPLVVRSKSGFMADASGAVRVDAGIVEI
                     ADGPRWVIALMTEGNPDRRFHPEHPGMVLNGRISRLVYDAWGTEVES"
     gene            275..826
                     /gene="tpx"
                     /locus_tag="VFV93_17465"
     CDS             275..826
                     /gene="tpx"
                     /locus_tag="VFV93_17465"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX5167199.1"
                     /translation="MTVERTGEAHELGEQLTVVGAQLQPGDAAPDFTLEYMDPSDNAL
                     RQVRLADSAGQVRLLNVINSIDTPVCHVETRRWERRRRELPESVLVLTVSMDLPFAQA
                     RWHSAEQVSHKSLSAHKSEQFGIDYGVLIKEWRLLQRSVFVIDGNGRIVHCEYVADQM
                     LEPDYDAAIAAALATAKADATRS"
     gene            1048..2370
                     /locus_tag="VFV93_17470"
     CDS             1048..2370
                     /locus_tag="VFV93_17470"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX5167200.1"
                     /translation="MSELAIATTIFVVTYAVIISERLDRSVVALAGGALMIALGILDQ
                     EEATAAIDFNTLALLIGMMIIINVMKRTGVFRYVGWRTASAVGGNPWKLMLGFACFTA
                     FASAFLDNVTTILLMVPVTIAICDDLGLDAKPFLITQVVASNIGGTATLIGDPPNILI
                     GSATGYDFMAFLENLAPLVVLLLGLTFVGYWLMYGRGDQLGVVTAAAKETLEKSDARV
                     HISDDGLLVRSLVVLGLTIVGFIFHGVLHFEAGSIAMFGAIALLLVSRADLHGILGEV
                     EWGTIFFFVGLFVLVGGLVAVGLLDKIARQVISLTDGNVTLTVLLILWFAAVASAVVD
                     NIPAVATMIPLTFSIARLLFPDLAGMDNVALAAHPDVAPLWWALALGACLGGNGTLVG
                     ASANVVAVGIAERRGEEIGFWGFTRIGLPFTIMTLIVSSIYLWLRYLA"
     gene            complement(2392..3921)
                     /locus_tag="VFV93_17475"
     CDS             complement(2392..3921)
                     /locus_tag="VFV93_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167201.1"
                     /translation="MPEGTPPIRRDAGSDRTERLNAAIDRMLAGGPSPVLNDPDLFEL
                     LQLAARLRDELPGDLPDPAFRLDLKQQLTANGPRAVPRQPATPVSRFPWVSAVSAIAA
                     VLLAAVSVGALGIWIDTDKQPSGQSNLAGISADQLTATTFGIATFTAETIAATSDAAT
                     TPGGDRPSSMTALQPTSPATSAPDSTEAVATLSAATETPGSDTTRTTATVAPTGTSDA
                     PSLASVPPVDSETVEQGPQPASDGGGGEPAAGISYVLDTALPDLGNDAAIYRLAPPAV
                     EPELFVADVAKSLGLKGEIISDAPQGRAVYHIFDDSAGSFHWTPETGAFTLSTAEADN
                     GDPVPLDQVVHATRQWLSKIGYPVDQLATDMSAEPMGATSWRLEARYASMPDIGLGHP
                     LGVTIFVNDDGAITEASGYWLRIKDADSALLLPADDVWQEVSSGHGLWTGGGIVTGGG
                     EFRADQIQITYILTRDPSGDLVLQPVVQVTGDFTTPDGLSTARISCFIQAARTTDDNS
                     P"
     gene            complement(3914..4513)
                     /locus_tag="VFV93_17480"
     CDS             complement(3914..4513)
                     /locus_tag="VFV93_17480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5167202.1"
                     /translation="MTGAGDRYNIEPVPGSSDEVDEGALVAAAKRDPREFGPLYELYV
                     DRIYRFAYRRVGTHHEAEDITAQTFQQALQALPAYEWRGLPFGAWLFRIAGNIINRRG
                     RTSGREVAVEDVTVFSGYEEMDDDPADQVWRSEEAGELAEKIKRLPPDQQRVLVLKFS
                     HGMKNREIGDLMGRSEGAIKQLVHRALVSLRASMERTYA"
     gene            4800..5327
                     /locus_tag="VFV93_17485"
     CDS             4800..5327
                     /locus_tag="VFV93_17485"
                     /inference="COORDINATES: protein motif:HMM:NF040503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HEX5167203.1"
                     /translation="MRQQLDATQVTVRPAREADAASIAEIYNQGILGRQATFETRLRT
                     PDDILETLRAGAGRFPYLVAEIDGVVAGWASVSTYRPRDCYAGIGEFSIYVHEDWRHA
                     GVGRVLLPALIAAAEQAGFWKLLSRVFPENTGSLRLAAACGFREVGTYEKHGQLDGVW
                     KDTVIIERLIAANQG"
     gene            complement(5538..5891)
                     /locus_tag="VFV93_17490"
     CDS             complement(5538..5891)
                     /locus_tag="VFV93_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167204.1"
                     /translation="MHYFRIYADEHGETHFEDLEIATDQPSNGSALSALIPANGVIFR
                     RSPADQYIDWHPAPRRQFVITLAGHAEVEASDGEVRQIGPGTIMLADDTTGKGHITRG
                     TGTEERLSVFIPLAD"
     gene            complement(5958..7325)
                     /locus_tag="VFV93_17495"
     CDS             complement(5958..7325)
                     /locus_tag="VFV93_17495"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX5167205.1"
                     /translation="MSGAHVTIRPADWRPDRRQLLILLALVTIALAVRLPFVHESGLP
                     SDIKLFVAWSETAANQGFAQLYARDDFNYPPVASYIFVLVGHLQRDMLEQPFTSESRS
                     MRAALKLPAILADLATTAVLYLFLARRTSLVTALAGALAYALNPAIIYDSLIWGQWDS
                     LVALPMLMATICLIAASPVLATIFLTIAILIKFQAVVLIPIGIVVIQRQFGWRGLGRS
                     ALTSVVTATILLLPVIVTGQVRQTMHIYAGLTHSQPWLSSNAFNVWWLVNWLQSGSPE
                     TRLKSHDLLLGPVDYKLVALCLYALAVVGIAAALTRARLTVETISLAAAASVLAFFAL
                     APEMHERYLLPALPLLILGIDTRLNRWLLAILSLTVFLNLSWVLRAASGDPSSALQLA
                     ATGLLAFVNLAALIWIYVALYRRVVDRPACAHRANVVGASAVLAVAALAGVTILRWYF
                     RDIRG"
     gene            complement(7322..8539)
                     /locus_tag="VFV93_17500"
     CDS             complement(7322..8539)
                     /locus_tag="VFV93_17500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5167206.1"
                     /translation="MDFTFSDNDQQLLERAKALATEFATRARAYDQDAAFPEEDFRRL
                     REEGFLTLAVPEELGGHGMWQGNRFVIYYTILETLAAGNASTAQLVQIQSHATGIIAN
                     LASEPLKRRVLTEVVERGTLVASCGSETDPNQMASKDGRAEMQAVDGGFRLTCTKHFA
                     SLAPEADYFVVYVMAPGTPTAAAGLTTVLVSKDDPGVSLQNNWDTLGMRATISWGLVL
                     DNVFVPWERVMGQPGDWVQHDPRTFTLGYVANHLGTAQGVYDFVLDYLKQRPFLTNDD
                     TIMYTVGEMDAALQATRTSMWYAAWLWEQARYTDAELASMRALHTSKQAALMITTKAF
                     DVVGARAAFRHLPVERAFRDVRTFSLHFRESALLKLLTQANLGADFHSKQRYGPNIGR
                     QSWETLEAVERDD"
     gene            complement(8762..>9085)
                     /locus_tag="VFV93_17505"
     CDS             complement(8762..>9085)
                     /locus_tag="VFV93_17505"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5167207.1"
                     /translation="FETRLPHQLSLGQRKRISLATVLAMDPRILVLDEPSAGLDPRAR
                     RGLITLLDSLPQTLIASTHDMRLVRDLFTRAVIMQEGRIVIDAPTSDIIDDESLLEAY
                     GLELP"
BASE COUNT         1553 a         2610 c         3310 g         1612 t
ORIGIN      
        1 cgcggctgct gccgttccac cagtacgcgc gggatggata cacgcacagc gggccactgg
       61 tggtgcgctc gaagagcggc ttcatggccg acgcgagcgg ggcagtgcgg gtcgatgccg
      121 gcatcgtcga gatcgccgat ggaccgcgct gggtgatcgc gctgatgacg gaaggcaatc
      181 ccgatcggcg cttccaccct gagcaccccg gtatggtgct caacggtcgg atttcccggc
      241 ttgtctacga tgcgtgggga acagaggttg agtcatgacg gtcgagcgta caggcgaggc
      301 gcacgagctc ggcgagcagc tgacggtcgt cggcgcgcag ttgcagccgg gggatgcagc
      361 gccggatttc acgctggaat acatggaccc aagcgacaac gcgctgcgac aggtccggct
      421 ggccgactcg gccggtcagg tccggttgct gaacgtcatc aactcgatcg atacgccggt
      481 ctgccacgtc gagacacgcc gctgggagcg ccgccgccgc gaattgccgg agtcggtact
      541 cgttctgacc gtcagcatgg acctgccgtt tgcgcaggcg cgctggcaca gcgcggagca
      601 ggtgagccac aaatcgctct cggcgcacaa gagcgagcag ttcgggatcg actacggcgt
      661 gctgatcaag gaatggcggt tactgcagcg ctcggtcttc gttatcgatg gcaatggccg
      721 gatcgtccac tgcgaatacg tcgcggatca aatgctggag ccggactacg acgcagcgat
      781 cgcggcggca ctggcaacgg ccaaggcgga tgccacgaga tcatgaggga aggggcctgt
      841 tgccggactc cgccgagatc gcgtgccggg ggatcgtggg gccggtgatg aggggagaac
      901 gcccgccaag gcgctgacgg cggcgtggta ggcttgcggt ggcgcgtttg cgccgggggg
      961 tgtcatggac cctgtatcca catgactcgg tggggactgt cacgagacag tctctttttt
     1021 gtgcctgcga tccggggaag aggcgcgatg tcggaactgg cgatcgcgac aacgatcttt
     1081 gtcgtgacgt atgcagtcat catcagtgag cggctggatc ggtcggtcgt cgcgttggcg
     1141 ggcggggcgc tgatgatcgc tttgggcatc ctcgatcagg aggaggcaac ggcggcgatc
     1201 gacttcaata cgctggcgct gctgatcgga atgatgatca tcatcaacgt catgaagcgg
     1261 acgggcgtct tccgttacgt cggctggcgg acggcgagcg cggtcggcgg aaacccgtgg
     1321 aagctgatgc tggggttcgc ctgcttcacg gcattcgctt cggcgttcct cgacaacgtg
     1381 acgacgatcc tgctgatggt gccggtgacg atcgcgatct gcgatgacct ggggctggac
     1441 gccaagccgt tcctgatcac ccaggtggtc gccagcaata tcggtgggac agcgacgctg
     1501 atcggtgatc cgccgaacat cctgatcggg agcgcgaccg gctacgactt catggcgttt
     1561 ctggagaacc tggcgccgtt ggtcgtgctg ttgctggggc tgacgttcgt gggctactgg
     1621 ctcatgtacg ggcgcggcga ccagctcggg gtcgtgacgg cagcggcgaa agagacgctg
     1681 gagaagtcag acgcacgggt ccatatcagt gacgacgggt tgctggtgcg ctcgctggtc
     1741 gtgctggggc tgacgattgt cgggttcatc ttccatggcg tgttgcactt cgaggccggg
     1801 tcgatcgcga tgtttggggc aatcgcgcta ctgctggtga gtcgggccga cctgcatggc
     1861 attctgggtg aggttgagtg gggcacgatc ttcttcttcg tggggttgtt cgttctggtc
     1921 ggcgggctgg tagccgttgg gctgctggac aagatcgcgc ggcaggtcat ctcgctgacg
     1981 gatgggaatg tgacgctgac ggtgttgctg atcctctggt tcgcggcggt ggcatctgcg
     2041 gtcgtggaca acatcccggc tgtcgcgacg atgatcccgc tgacgttttc gatcgcccgg
     2101 ttgctgtttc cggatctggc agggatggac aatgtcgcgc tggcggccca tccggatgtt
     2161 gcgccgttgt ggtgggcgct ggcgctcggc gcgtgtctgg gcgggaacgg gacgctggtg
     2221 ggagcctcgg cgaatgtcgt tgcggttggg atcgcggagc gacgcgggga ggagatcggg
     2281 ttctggggct tcacccggat tggactaccg ttcacgatca tgaccctgat cgtgtcgagc
     2341 atctacctct ggctgcggta tctggcgtaa cctggacaac cattgggttc gttacggcga
     2401 gttgtcgtca gtggtacggg ctgcctggat gaagcaggag atgcgggcgg ttgagaggcc
     2461 gtcgggagtg gtgaagtcgc cggtgacctg aacgaccggc tggaggacga ggtcgccgga
     2521 gggatcgcgg gtcaggatgt acgtgatctg gatctggtca gcgcggaact cgccaccgcc
     2581 ggtgacgatg ccgccgcccg tccagaggcc atggccgctg ctgacctcct gccagacgtc
     2641 gtcggctgga aggagcagcg ccgaatcggc gtccttgatg cgcagccagt agccgctggc
     2701 ctcggtaatc gccccgtcgt cgttgacgaa gatcgtcaca ccgagcggat ggccgaggcc
     2761 gatgtcgggc atcgacgcat agcgggcttc gaggcgccag ctggtggcac ccatcggctc
     2821 ggcgctcatg tcagtcgcga gctggtcaac cgggtagccg atctttgaga gccactggcg
     2881 agttgcatgc acgacctgat cgagcgggac cggatcaccg ttgtccgctt cggcggtcga
     2941 gagcgtgaag gcgccggttt caggcgtcca gtggaatgag ccggcgctgt cgtcgaagat
     3001 gtggtagacg gcgcggccct gcggcgcgtc gctgatgatc tcgcccttga gccccagcga
     3061 cttggcgacg tcggcgacga agagttccgg ctcgaccgcc ggcggggcga gccggtagat
     3121 ggcggcgtcg ttgcccaggt caggcagggc tgtgtccagc acataggaga taccggccgc
     3181 cggctcgcca ccgccgccgt cggaggcggg ctgtggtccc tgctcgaccg tctcggaatc
     3241 gaccggcggg accgaggcca gcgatggggc atcactcgtt cctgtcggag cgacggtcgc
     3301 cgtcgtgcgg gtcgtgtctg aaccgggcgt ctcggtcgca gcggagaggg tcgcgaccgc
     3361 ctccgtcgaa tccggagccg acgttgccgg gctggtgggc tgcagggcgg tcatgctcga
     3421 cggacgatcg ccgcctggcg tcgttgccgc gtcggaggtc gccgcgatcg tctcggccgt
     3481 gaaggtagcg atgccgaagg tggttgccgt caactggtcg gcagagattc cggccagatt
     3541 cgattggcct gatggttgct tgtcggtgtc gatccagatc ccgagcgcac cgacgctgac
     3601 ggcggccagc aggacggctg cgatggcgct gacggcagaa acccatggga accgggaaac
     3661 cggagtcgca ggttgacgtg ggaccgcgcg cgggccgttg gcggtgagct gctgcttgag
     3721 atcgagccgg aaggccgggt cgggcagatc tccgggcaat tcgtcgcgca ggcgggcggc
     3781 aagctggagg agctcgaaca ggtcggggtc gttgaggacg ggagaggggc cgccggccag
     3841 catccggtcg atggctgcgt tgagacgctc ggtgcggtcg ctaccggcgt cgcgacgaat
     3901 cggcggtgtg ccctcaggca tacgtgcgct ccatcgaggc acgcaggctg acgagtgcgc
     3961 ggtggacgag ttgcttgatg gcaccctctg agcggcccat cagatcacca atctcgcggt
     4021 ttttcatgcc gtgcgagaac ttcaacacca ataccctctg ctggtcaggc ggtagccgct
     4081 tgatcttctc ggccagttcg cccgcctcct cggatcgcca gacctgatcg gccggatcgt
     4141 cgtccatctc ctcgtagccg ctgaacaccg tgacatcctc gaccgcaacc tcgcgcccac
     4201 tcgtccggcc gcgccggttg atgatgttgc cggcgatccg gaacagccag gccccgaagg
     4261 ggagcccgcg ccactcgtag gccggcagcg cctgcagcgc ctgctgaaac gtctgggccg
     4321 tgatgtcctc cgcctcgtgg tgcgtcccga cgcgccggta ggcgaaacgg tagatccggt
     4381 ccacgtacag ttcgtagagg ggaccgaact cgcgcgggtc acgcttggcg gcagcaacca
     4441 gcgcgccttc gtcgacctcg tcggacgagc cgggaacagg ttcaatgttg taccggtctc
     4501 cggcgcccgt cacgcgggtt caccccttta cgtcagcaca atccgaatga tattgcaggg
     4561 ttacgcggga accaccctgc cggccggaat cgctcgcccg gcgccactgg ccaatcgcga
     4621 atagcccact gccggaatcc gcgacaccat caatggctag acgtgccaca ccccacttgt
     4681 agttcccgca cgagcaacgc gggtcacgtt tgggcaggcc gacgatggcg cagcataccc
     4741 ccgaacgtat gatgcatgat gaacctgagg agaagatgaa tgaacgcgaa gggggagcga
     4801 tgaggcagca gctcgatgcc acgcaagtaa cggtccggcc ggcacgggaa gcggacgcgg
     4861 cgtcgatcgc cgagatctac aaccagggca tcctcggccg gcaggcgacc ttcgagacgc
     4921 ggctgcgcac gccggacgac atcctcgaga cgttgcgagc tggtgcgggt cgattcccct
     4981 acctggtcgc ggaaatcgac ggcgtcgtgg ccggctgggc gagcgtctcg acctaccggc
     5041 cgcgcgactg ctatgcgggg atcggcgagt tctcgatcta tgtccacgag gactggcgac
     5101 acgcgggcgt cggtcgggtg ctcctgccgg cactgatcgc cgccgccgag caggccgggt
     5161 tctggaagct gttgagccgg gtcttccctg agaacaccgg cagcctgcga ctggcagccg
     5221 cttgcggctt ccgcgaggtc ggaacctacg agaagcacgg ccagctcgac ggagtctgga
     5281 aagacacggt catcatcgaa cgcctgattg cggcgaatca agggtagggc ttcgggacgc
     5341 gggacgtgtg acgcgatcca gttcaacgct gttcgcctgg atcgttttct tggaagtggg
     5401 aatagagcgg caagctcgag tgtttgagtg aatgaccgag tccgggggtg tctggaagga
     5461 cacatgtgtg gcggccgtgt tgccggatag caccctcggg cagaggtctc tctttttgcc
     5521 actcttcctg cgctgtttca atctgccaac ggaatgaaga ccgagaggcg ctcttcggtg
     5581 ccagtgccgc gcgtgatgtg gcctttgccg gtcgtgtcat cggcgagcat gatcgtgccg
     5641 gggccgatct ggcgaacttc gccgtcgctg gcctcgactt cggcgtggcc ggccagagtg
     5701 atgacgaact ggcgacgggg ggccgggtgc cagtcgatgt actggtcggc gggactgcgc
     5761 cggaagataa cgccgttggc ggggatcagt gccgagaggg ccgagccgtt gctgggctgg
     5821 tcagtggcga tctcgagatc ctcgaagtgg gtttcaccgt gctcgtcggc gtagatgcga
     5881 aagtagtgca tggtccctcc tggtgcgcgg gtggcgatcg agtgcgcatt cgcggacggt
     5941 atcgtacccg ctttgggtca gccgcgaatg tcacggaaat accagcgcag gatggtgacg
     6001 ccggccaggg ccgcaaccgc cagaaccgca gatgcgccaa ccacattagc gcggtgggcg
     6061 catgcggggc gatcgacaac tcgtcggtac aacgcgacgt agatccagat cagggctgcc
     6121 aggttcacga acgcgagcag tccggtcgcg gcgagctgca acgccgagga tgggtcaccg
     6181 ctagcggcgc gcaggaccca actgaggttg aggaagaccg tcaggctcag gatcgcgagc
     6241 agccagcggt tgagccgggt gtcgatcccc aggatcagga gtggcagggc gggcagcagg
     6301 tagcgttcgt gcatctcggg agcgagcgcg aagaaggcaa gcaccgacgc tgccgcggcc
     6361 aggctgatcg tctccaccgt cagccgggcg cgggtgagcg cggcggcgat tccaacgacg
     6421 gccaacgcat acaggcacag tgccacaagc ttgtagtcga cagggccgag cagcagatcg
     6481 tgactcttga gacgtgtctc cgggctaccg ctctggagcc agttgaccag ccaccagacg
     6541 ttgaaggcgt tgctggataa ccacggctgg ctatgggtca gcccggcgta gatgtgcatg
     6601 gtctggcgaa cctggccggt gacaatcacc ggcaggagca ggatcgtggc ggtcacgaca
     6661 ctggtcaatg ctgacctgcc caggccgcgc cagccgaatt ggcgttggat gacgacgatg
     6721 ccaatcggga tgaggacgac cgcctgaaac ttgatcagga tcgcgatcgt caggaagatg
     6781 gtcgcgagga cgggactggc cgcgatcagg caaatcgtcg ccatcagcat cggcagcgca
     6841 accagcgaat cccactgccc ccagattagc gaatcgtaga tgatcgctgg attcagcgcg
     6901 tacgccagcg caccggcaag ggcggtgaca agcgacgtgc ggcgcgccag aaagaggtac
     6961 aggacggcag tcgttgccag gtccgcgagt atcgctggga gcttgagggc ggcccgcatg
     7021 gacctgctct cagaagtgaa cggctgttcc agcatgtcgc gctggagatg accgacgagg
     7081 acgaagatgt agctggcgac tggcggatag ttgaagtcgt cgcgggcgta gagttgggcg
     7141 aatccctgat tggcggccgt ttcggaccag gcgacgaaga gtttgatatc gctgggtaag
     7201 cctgactcgt ggacgaatgg cagccggacc gccagcgcga tcgtgacgag cgccagcagg
     7261 atcagcagtt gacgccgatc aggtcgccaa tccgctggac ggatggttac gtgggcgccg
     7321 ctcagtcgtc tcgttcaact gcctccagcg tctcccacga ctggcggcca atgttcgggc
     7381 cgtagcgctg cttggagtgg aagtcggcgc cgaggttggc ctgcgtgagc agcttgagaa
     7441 gtgcggactc gcggaagtgg agcgagaagg tgcggacgtc gcggaaggcg cgctcgacgg
     7501 gcagatggcg gaaggcggcg cgggcaccga cgacatcgaa ggctttggtg gtaatcatca
     7561 gcgccgcctg cttcgatgtg tgcagggcgc gcatcgaggc cagctccgcg tctgtgtagc
     7621 gggcctgctc ccagagccag gcggcgtacc acatcgacgt tcgagtcgcc tggagggcgg
     7681 cgtccatctc gccgacggtg tacatgatcg tgtcgtcatt ggtgaggaac gggcgctgct
     7741 tgagatagtc gaggacgaag tcgtagacgc cctgggcggt gccgaggtga ttggcgacgt
     7801 agccgagggt gaaggtgcgc gggtcgtgct ggacccagtc acccggctgg cccatgaccc
     7861 gttcccaggg gacgaagacg ttgtcgagga cgagccccca actgatggtc gcgcgcatgc
     7921 caagcgtgtc ccagttgttc tgcagcgaga cgccggggtc gtcctttgag acgagaacgg
     7981 tcgtcaggcc ggctgcggcc gtcggcgttc cgggggccat cacgtagacg acgaagtagt
     8041 cggcctccgg cgcgagtgag gcaaagtgct tggtgcaggt cagccggaag ccgccgtcaa
     8101 cggcctgcat ctcggcgcgg ccatccttgg aggccatctg gttcgggtcg gtctcgctgc
     8161 cgcaggaggc gaccagcgtt ccacgctcga caacctcggt caggacgcgg cgcttgagcg
     8221 gctcggaggc gaggttggcg atgatgccgg tcgcgtggga ctggatctgg acgagctgcg
     8281 cggtcgaggc gttgccggcg gcgagcgttt cgaggatcgt gtaatagatg acgaagcggt
     8341 tgccctgcca catcccgtgg ccgccgagtt cttcaggtac agcaagcgtg aggaagccct
     8401 cctcgcgcag gcggcgaaag tcctcctcag ggaaggcggc atcctgatcg taggcacgcg
     8461 cgcgggtggc gaactccgtg gcaagcgcct tggcgcgttc cagcaactgc tggtcgttgt
     8521 cggagaaggt gaagtccacc cgtgtgcctc ctgtcgcact gccgcatggg aatcacgtga
     8581 tggtgtgctg tatacggcag ggttagttca gagtcaacaa gggcgggatt cgggattcgg
     8641 gacgcgggac gtgggacgcg ggatgtgtcg caaccacgcg acgcaccgag gatcgtcacg
     8701 ccttgcccgg tcatcctgag cgccagcgaa ggatctcgtt gctcgttggc gcgacgagtg
     8761 gtcaggggag ctcgaggccg tatgcttcga ggagagattc gtcgtcgatg atgtcggaag
     8821 tgggtgcgtc gatgacgatt cggccttcct gcatgatcac ggcgcgggtg aagaggtcac
     8881 ggacgaggcg catgtcgtgg gtggaggcga tcagcgtctg cggaaggcta tcgagcagcg
     8941 tgatcaagcc acgacgggcg cggggatcga gaccggcgga cggctcgtcg agaacgagga
     9001 tgcgggggtc catcgccagg acggtcgcga ggctgatgcg cttgcgctgg ccgagcgaga
     9061 gctggtgggg caggcgcgtc tcgaa
//
LOCUS       DASUGJ010000774         8030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_822931, whole genome shotgun sequence.
ACCESSION   DASUGJ010000774 DASUGJ010000000
VERSION     DASUGJ010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822931"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1743)
                     /locus_tag="VFV93_17510"
     CDS             complement(181..1743)
                     /locus_tag="VFV93_17510"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX5167208.1"
                     /translation="MSLKSALLPSESGNAALQTEVPQPTRAANRTVISLAIFLGVSLL
                     YVAISRGVFLYGDDILMFQVTESIVEPGDVAVTSPWHEGDIARAIPGRGGKGYSKYGV
                     GQSLVAIPAYVASDLILVKLLPIHVFIDGYGNQRMGAIVYGTALTNTVIGGATVALVY
                     LLAVACGFSRRTALVVAGLLAFGTLLAHYSATFLSEPLTGLALTGTVYGLVRSDSDGA
                     TGPTRYGYRWLAFSGFMAGLALGSRVATGLMLIAPGLWLLWLAWRWSQRSRRDAILAL
                     GVWGVPVVLWLIGIAWYNWVRFGSVSETGYGSEWQQMTTPFLTGLSGLLISPGKGLLW
                     YNPPLLLALAGGIWFARRRRDLTLVIAGMLVGALLLYSRYYVWYGGGVWGTRFLVPML
                     PLLVLPAGEIVERAWHGRRAFAVATGVIAAVGLFVTALGIMVPFDRYVGEYNVTPEQH
                     DAAIWEIADSPLVVHAERLDDTWRDPDIAADRYASKSLVAISLVTGFAGLGSLVLATR
                     GVMRGRFPKAPG"
     gene            1940..4399
                     /locus_tag="VFV93_17515"
     CDS             1940..4399
                     /locus_tag="VFV93_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167209.1"
                     /translation="MNRLLANKYQPWIVAVLFVVVTIWMTWPLTPHATRAVQDPGDPL
                     FEIWVMRTVQHRLVHNPLDLYDSNAFYPFDDSLAYSEEAISTALLAWPVYLLTGNDVL
                     AYNVVLLSAFWLVAFAVYLLARELGASPGAAFVAGILASFAPARYAHLSHLHMLVIGW
                     LPLALWALTRYVRGGRDRYLVLVAVALAIEFLASLHMAVFSTLVLALYLPFLLWFERR
                     ERGWERPDLLRLAAALIVPYLVLAPTLLPHVQAGHEYKMSRPRWEVEALSSEPSAYIS
                     TYVTNGFWNGPLRTASEPFFPGLVALAGALLALLVWRRWWVWFAAALTVAAAVISFGF
                     DIELAGRTITMPYALVYKLVPPIRDVRGVGRFGLLTAIGLPLLAAFGYSALWRRIRDR
                     AGEYVVPVGVGLTALLAVVACIELRAPARIDAPPDARTMAVYDWLADQPDGPVAEFPA
                     NGLLVPTTQPPGGLFQPIQYMYGSTRHWKPILSGYSGFIPAPHIFLMNHFDNRDDPGR
                     QSMVTERNVGLLQELDIRWVVFHRLPTYDIDTALALADTLPELRRVTEVGNSVVYELT
                     PETREPLPAEATTVEIRGDASAGGLLPVHFNVTNPHDNLSILHFDALPDLTSRWRRDD
                     GSVAKTERFEVPIPTVVNPGSTSIEALLTAPDEPGIYTVEISLDRTDVADASRQVEVD
                     AAVIGDNQILRLESVDWDRSASPQPGDHVQVEVTWAVLERPSANFSATLQLLNAAGER
                     VAGSDLLPGAGMPPTSEWTPGQRVTLSFDLVIDAGLPPGDYQLLTAVYAYRPDFPRLR
                     IERTDGTTATEAILPGFTIGR"
     gene            4460..7465
                     /locus_tag="VFV93_17520"
     CDS             4460..7465
                     /locus_tag="VFV93_17520"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5167210.1"
                     /translation="MWTTSAGIRRATTSERVEMHVLIVADDLKSRTAVAEAAAVDGVT
                     VETAETLEAATRALTRGSFGLVIVRERLGDAAPADISSAVRATAGGVESRLVAMAASR
                     KPARQVLSAAGFDDVLDLPVNPDQVRSQVVRAGRSLRQRETLRKRDEQLEILNTLAYY
                     LSRSNSVERQVQHALQAVSQLYPYGRAAIWLRNDDGCVSLGRSIGLSHTYQAAAEPVY
                     GRMTAVQWSALGSRPRFITNPEAVGDELRDLAASEGFAASLDIALIATSGVMGTLVLY
                     FDEPAAPADDEVTLLETIAATTAMAIVTGRLAGEMRQPGDADETYSEIFEHLPDPVFM
                     HDADGHFVLSNPAMLALSGYPESQLEQHTIYELFSGDDADLIGDQVSYLLHLARGDEP
                     TFSVIGPLPVDLMRADRQPVHADLYLRSLRLPHLGNDVYIQGIVRDVSENERVRQELN
                     ANQTIVRIMAEAPDLDTAMIQVWDIMTLQLGYTRGGIWTVSPDGSELVTRAAAEGFSL
                     STLSIKRGIIGRVARERKAVFIRDVRDDPDYISIDPAVVSEICVPILRDDRIIGVIDV
                     EADETQPLEERDLSFLRSLATQLAGLIERAELHDNLERQASTDLVTGLPNRQVFQERL
                     DNAVAAARHGKVSMLMIGVDNFKGINELYGHISADDVLRQIARALDARISDSQVLARY
                     TSDTFTVLLPGINRDQAVTIAENLRIGVAMQLFMAAEQVEQVTVSIGAATYPDDATSA
                     GELLQAADHAMYLAKRAGRNQTFQSNKAFATLAPHHGRITDLLRQSPKETLSMLVRAM
                     DQRLPERKGHSERVTRYTMAIARQLNMPDTELPRLRLAAYIHDIGMATLPDSLLRKPG
                     RLSPEEREQLRNVPLEARDLLAQLDISDSTRQAVVHQRENWDGTGHPDQLRGQQIPLG
                     ARIIAVADAIDAMTSARAHREPMTIEEALEQVNLQAGTQFDPDVAAAAQVLTSIIKPP
                     APAAEPQPTDDDEADELAETLEPVTSSDE"
     gene            complement(7597..>8030)
                     /locus_tag="VFV93_17525"
     CDS             complement(7597..>8030)
                     /locus_tag="VFV93_17525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5167211.1"
                     /translation="VPGFDAPDLADLSRQVWTAGRTDGVEAAKALWLACPFFAQANRR
                     SDAKAALQQMVTDYSGWGWTERDPGTWGEPNMAARLGEISAPALIVVGERDIEGMRRA
                     ADALAREIPNAQKVVLPGLGHLPNMEDPAAFNAAVLKFLAG"
BASE COUNT         1412 a         2813 c         2530 g         1275 t
ORIGIN      
        1 acccccaccg ggacccgctc cgtaatcgcg tcctctacac tgatcgtcgt tgtctcgtct
       61 gccaaagccg cctccctcac taccgccatc gttgggcgca gtatcccacg agagccaccg
      121 gccgtcagcg gatggtttgg gggctttgat gtgagaggag gcagaacgta cgaggagcga
      181 ctatccggga gccttcggaa agcgtccacg catgacgcca cgggtcgcca ggaccagcga
      241 gcccagccca gcgaaccccg tgaccaatga gatcgcgacc aggctcttcg acgcgtatcg
      301 atcggcggcg atatccggat cgcgccaggt atcgtcaagt cgctccgcat ggacgacgag
      361 cggggagtcg gcgatctccc agatcgccgc gtcgtgctgc tcgggcgtca cgttgtactc
      421 accaacgtag cggtcgaacg gcaccatgat cccgagcgcg gtaacgaaca ggccaacagc
      481 cgcgatgacg ccggtcgcca cggcgaaggc gcgccgtccg tgccaggccc gctccacgat
      541 ctcgccggcc ggcaacacca gcaacggcag catcgggacg aggaaccgcg tcccccagac
      601 gcccccgccg taccagacgt agtagcggct gtagagcagc aacgcaccga ccagcatgcc
      661 ggcgatcacc agcgtcagat cgcgccgccg ccgtgcgaac cagatgccac cggccagcgc
      721 cagcaggagc ggcgggttgt accagagaag ccccttcccc ggagaaatca gcagccccga
      781 caggccggtc agaaacggcg tcgtcatctg ctgccactcg gagccatagc ccgtctcgga
      841 cacgctgccg aagcgcaccc agttgtacca ggcgattccg atcagccaga gcacgaccgg
      901 cacgccccag acgccaaggg ccaggatggc gtcacgccgc gaacgctgcg accagcgcca
      961 cgcaagccac agcagccaga gccccggcgc aatcagcatc aagcccgtcg cgacccgcga
     1021 tccgagcgcc agccccgcca tgaaccccga gaatgccagc cagcggtagc cataacgcgt
     1081 cgggcccgtt gcgccatcgg agtccgagcg gacgaggccg tagaccgtgc ccgtcagcgc
     1141 caggccggtc agcggctcgg agaggaaggt cgccgaatag tgggccagca acgtcccgaa
     1201 cgcgagcaaa ccggccacaa cgagcgcggt ccggcgcgag aagccgcacg ccaccgccag
     1261 caggtacacc agcgcgaccg tcgccccacc gatgacggtg ttcgtgagtg ccgttccata
     1321 gacgatcgcg cccatccgct gattaccgta gccatcgatg aacacatgaa tcggcagcag
     1381 cttcacgaga atcaggtcgc tcgcaacgta cgccgggatg gcgactaacg actgaccgac
     1441 accgtacttg gagtagccct tccctccacg tcctggaatc gcgcgggcga tatctccttc
     1501 gtgccagggc gatgtgacag ctacatcgcc aggttcgacg atgctctcag tgacctgaaa
     1561 catgaggatg tcgtcgccgt agaggaagac gccgcgtgag atggcaacgt acagcaggct
     1621 gacgcccagg aagatcgcga gagagatcac ggtgcggttg gcggcgcgtg tgggctgggg
     1681 aacctctgtt tggagtgcgg cgttgccgga ttcagatggc agaagggcac tcttgaggct
     1741 caatgattct ccgggtccac gaagacgctg ggtgcgtgag acactccgca accgagcgtt
     1801 ctgaatccat gcaagcgtaa ccatacacgc tgagcggtcc acctctgtca tcgtgtgcgg
     1861 tgggccactg agtcgtgagc cgaagtgact gaccgaccgg ctgggagcca tctgtgcggt
     1921 tgattatgct tatggtgtgt tgaaccgtct gctggccaat aagtatcagc cgtggatcgt
     1981 cgcggtgttg ttcgtcgtgg tgacgatctg gatgacgtgg ccgttgacgc cgcacgcgac
     2041 ccgcgccgtc caggaccccg gcgatccgct gttcgagatc tgggtgatgc ggaccgtcca
     2101 gcaccggctc gtccacaacc cgctcgatct ctacgactcg aacgcctttt atccattcga
     2161 tgactcgctg gcctactccg aagaagccat ctcgacggca ctgctggcct ggccggtcta
     2221 cctgctgacc ggcaacgatg tgctggccta caacgtggtg ctgctgtcgg ctttctggct
     2281 ggtcgctttc gccgtctact tgctggcgcg cgagcttggc gcgagtccgg gcgcggcctt
     2341 cgtcgccgga atactggcct cgttcgcgcc ggcccgctac gcgcatctca gccacctgca
     2401 tatgctcgtc atcggctggc tgccgctggc gctctgggcg ctgacccgct acgtccgtgg
     2461 cgggcgcgat cgctatctcg tgctggtcgc cgtcgcgctg gcgatcgagt ttctcgcctc
     2521 gctgcatatg gccgtcttct cgacgctggt gctggcgctc tacctgccgt tcctgctctg
     2581 gttcgaacgt cgagaacggg gctgggagcg accagatctg ctccgcctcg ccgccgcgct
     2641 gatcgtgccg tacctggttc tcgcgccgac gctactgccg cacgttcagg ccggacacga
     2701 atacaagatg agccgcccgc gctgggaagt tgaggcgctg tcgtcggagc cgtcggcgta
     2761 tatctcaacg tatgtcacca acggcttctg gaacggaccg ctgaggacgg cgtcggagcc
     2821 gttcttcccc ggcctggttg cgctcgccgg cgcgctgctg gcgctgctgg tctggcggcg
     2881 ctggtgggtc tggttcgcgg cggcgctgac ggtcgccgcg gcggtcatct cattcggctt
     2941 cgacatcgaa ttggccgggc gaacgatcac gatgccctac gcgcttgtct ataagctcgt
     3001 cccgccgatc cgcgacgttc gcggcgtcgg ccgcttcggg ctgctgacgg cgattggtct
     3061 cccgctactg gccgctttcg ggtacagtgc cctctggcgg cgcattcgcg atcgggctgg
     3121 tgagtatgtc gtaccggtcg gcgtcgggct gacggcgcta ctggcggtcg tcgcctgcat
     3181 cgagctgcgg gcgccggcgc ggatcgacgc tccgcccgac gcgcggacga tggccgtcta
     3241 cgactggctg gccgatcagc cggatggccc ggtcgccgag ttcccggcca acggcctgct
     3301 cgtcccgacg actcagccac ctggcggcct ctttcagccg attcagtaca tgtacggctc
     3361 gacccgccac tggaaaccga tcctctccgg ctacagcggc ttcatcccgg cgcctcacat
     3421 cttcctgatg aaccacttcg ataaccgcga cgatccaggc cggcaatcga tggtgaccga
     3481 gcgcaacgtc ggcctgttgc aggaactcga catccgctgg gtcgtctttc accggctgcc
     3541 cacctacgat atcgacaccg cactcgcgct cgccgacaca ctaccggaac tacggcgcgt
     3601 gaccgaggtc ggcaacagcg tcgtctacga gctgacgccg gaaacgcgtg agccgttgcc
     3661 agccgaggcg acgacggtcg agattcgcgg cgacgcgtcg gccggtgggc tgctgccagt
     3721 ccatttcaac gtcacgaatc cgcacgacaa cctctcgatt ctccatttcg acgcgctgcc
     3781 cgatctcacc tcccgctggc gacgcgacga cggaagcgtc gcgaagaccg agcggttcga
     3841 agtcccgatc ccgaccgtcg tcaaccccgg ttcaacatca atcgaggcat tgctgaccgc
     3901 accggacgaa cccggtatct acaccgtcga gatcagcctc gaccggaccg acgtcgctga
     3961 cgccagccgg caggttgagg tcgacgccgc cgtcatcggc gacaatcaga tcctgcggct
     4021 ggagtcggtc gactgggatc ggtcggcatc gccacaaccg ggcgatcacg ttcaggttga
     4081 ggtcacctgg gcggttctcg aacggccgtc ggccaacttc tcggccacgc tgcaactgct
     4141 caacgcggcc ggcgaacggg tcgccggctc ggacctgttg cccggagccg gtatgccgcc
     4201 gaccagtgaa tggacgcccg gccagcgggt gacgctcagc ttcgacctcg tcatcgacgc
     4261 cggcctgccg cccggcgact atcaactgct gacggccgtc tacgcctacc ggccggactt
     4321 cccgcggctg cggatcgagc ggacggacgg tacgaccgcg accgaggcga tcctgccagg
     4381 attcacgatt ggacggtagc ggttcgaaaa tcaggacgaa acggacgatt gacgatcact
     4441 gaccggcaat gcacgcttca tgtggacgac cagcgccgga atccgccgag cgacgacgag
     4501 tgagcgagtt gaaatgcatg tcctgatcgt tgccgatgac ctgaaatcgc gaaccgccgt
     4561 cgccgaagcg gccgctgtcg acggtgtcac cgttgaaacc gccgagacgc tggaggcggc
     4621 aacgcgggcg ctcacacgcg gatcgttcgg gctggtcatc gtccgcgagc ggctgggtga
     4681 cgccgcgccg gccgacatca gcagcgccgt ccgtgcgacg gccggcgggg tcgagagccg
     4741 gctggtagcg atggccgcct ccaggaagcc cgcccggcag gtactctcgg cggcaggctt
     4801 cgatgatgtc ctcgatctgc cggtgaatcc cgatcaggta cggtcacagg tcgttcgcgc
     4861 cggccgcagc ctgcgccagc gcgagacgct ccgcaagcgc gacgagcagc tggagatcct
     4921 caacacgctc gcctactacc tctcgcgcag caactcggtg gagcggcagg tgcagcacgc
     4981 gttgcaggcc gtcagccagc tctacccgta cggccgggcc gcgatctggc tgcgcaacga
     5041 cgacggttgc gtcagtctcg gccgcagcat cggtctctca cacacctatc aggcggctgc
     5101 cgagccggtc tacggtcgga tgactgctgt ccagtggtcg gcgctcggca gccggccccg
     5161 cttcatcacc aacccggagg ccgtcggcga cgaactgcgc gatctggccg cctcggaagg
     5221 gttcgcggcg tcgctcgaca tcgccctgat cgccacctcc ggcgtcatgg gcacgctcgt
     5281 cctctatttc gacgagccag ccgctccggc ggacgacgag gtgacgctgc tcgaaacgat
     5341 cgcggcaacg acggcgatgg cgatcgtcac cggccggctg gccggcgaga tgcgccagcc
     5401 aggcgatgcc gacgagacct acagcgagat cttcgagcac ctgcctgatc cggtcttcat
     5461 gcacgacgcg gacggccact tcgtcctcag caacccagcc atgctggcac tctccggcta
     5521 tccggagagc cagctggagc agcacaccat ctacgagctg ttcagcggcg acgacgccga
     5581 cctgatcggc gaccaggtct cctacctgct acacctcgcg cgtggcgacg agccgacctt
     5641 cagcgtcatc ggcccgttgc cggtcgatct gatgcgcgcc gaccggcagc cagtccacgc
     5701 cgacctctac ctgcgctcgc tgcggctgcc acacctcggc aacgatgtct acatccaggg
     5761 gatcgtccgc gacgtcagcg agaacgagcg cgtccgccag gagctgaacg ccaaccagac
     5821 gatcgttcgc atcatggccg aagcgcccga cctcgacaca gccatgatcc aggtctggga
     5881 catcatgacc ctgcaactcg gctacacgcg cggcgggatc tggaccgtca gcccggacgg
     5941 cagtgaactg gtcacccgcg ccgccgccga aggcttctcg ctctcgaccc tctcgatcaa
     6001 gcgcggcatc atcggccgcg ttgcccgcga gcgcaaggcg gttttcatcc gcgatgtcag
     6061 ggacgacccc gactacatca gcatcgaccc ggctgtcgtc agcgagatct gcgtcccgat
     6121 cctgcgcgac gaccgcatca tcggcgtcat cgatgtcgaa gccgacgaga ctcagccgct
     6181 ggaggagcgc gacctgagct tcctgcgctc actcgccacg cagctggccg gtctgatcga
     6241 gcgcgccgag ctgcacgaca acctcgaacg gcaggcgtcg accgatctcg tgaccggcct
     6301 gccgaaccgg caggtctttc aggagcggct ggacaacgcc gtcgctgccg cccgccacgg
     6361 caaggtctcg atgctgatga tcggcgtcga caacttcaag ggcatcaacg aactctacgg
     6421 ccacatcagc gccgacgatg tactgcgcca gattgcgcga gcgctcgatg cccgcatcag
     6481 cgacagtcag gtgctggccc gctacaccag cgacacgttc actgtcctgc tgcccggcat
     6541 caaccgcgat caggccgtca cgatcgctga gaacctgcgc atcggcgtcg cgatgcaact
     6601 cttcatggcg gccgagcaag tcgagcaagt caccgtctcg atcggcgcgg ccacctatcc
     6661 cgatgacgcc accagcgccg gcgagctgct ccaggccgcc gaccacgcca tgtacctggc
     6721 caagcgcgcc ggccgaaacc agacgttcca gtcgaacaaa gcgttcgcca cgctcgcgcc
     6781 gcaccacggc cggatcaccg acctgctgcg acagtcgccg aaagagacgc tctcgatgct
     6841 cgtccgcgca atggaccagc gcctgccgga gcgcaagggc cactccgagc gcgtcacccg
     6901 ctacacgatg gcgatcgctc gtcagctcaa catgccggac accgagctgc cccggctgcg
     6961 gctggccgct tacatccatg acatcgggat ggcgacgttg cccgactcgt tgctgcgcaa
     7021 gcccggccgg ctctcacctg aagaacgcga gcagttgcgt aatgtcccgc tcgaagcgcg
     7081 tgacctgctg gcccagctcg acatctccga ctcgacgcgg caggccgtcg tccaccagcg
     7141 cgagaactgg gacggcaccg gccaccccga ccagctacgc gggcaacaga tcccgctcgg
     7201 cgcgcggatc atcgccgtcg ccgacgcgat cgacgcgatg accagcgccc gcgcccaccg
     7261 cgagccgatg acgatcgagg aagcgctgga acaggtcaac ctccaggccg gcacccagtt
     7321 cgaccccgac gttgcggcag ccgcccaggt gctgacctcg atcatcaagc cgccggcccc
     7381 agccgccgaa ccgcagccga cagacgacga cgaagcagac gagttggcgg aaacgctgga
     7441 gccggtcacc agcagcgacg agtagctcgc aacgtcaggc cgtcaagaag taggggccgg
     7501 tcttgtgcgc ggccctgctc ggttgcccaa atgcgtcggg tggggaacag ggccggcaca
     7561 agaccagccc ctacttcttg acgcgtatgc gctcggttag ccggcgagaa atttgaggac
     7621 agccgcgttg aatgccgccg ggtcctccat gttcggcagg tggccgaggc cgggcaggac
     7681 aaccttctgc gcgttgggaa tctcgcgcgc cagtgcgtcg gcggcccggc gcatgccctc
     7741 gatgtcgcgt tcaccgacga cgatcagcgc cggcgccgag atctcgccca ggcgggcggc
     7801 catgttcggc tcgccccagg ttccggggtc ccgctcggtc cagccccagc cggagtagtc
     7861 tgtgaccatc tgctgtagcg ccgctttcgc atcggaacgc cggttcgcct gcgcgaagaa
     7921 cggacacgcg agccagagtg ccttggcggc ctcaacgccg tccgtccggc ccgccgtcca
     7981 gacctggcgg ctcagatcgg cgaggtccgg cgcgtcgaag cccggcacga
//
LOCUS       DASUGJ010000775         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_962396, whole genome shotgun sequence.
ACCESSION   DASUGJ010000775 DASUGJ010000000
VERSION     DASUGJ010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962396"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..2596)
                     /locus_tag="VFV93_17530"
     CDS             complement(80..2596)
                     /locus_tag="VFV93_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167212.1"
                     /translation="MTSFRPSEHARLAKPRITRRQLLASAALSAAGVAASILVRNDPE
                     SDLDTLGRLTEPVTSAMTGDKPSEPAKTAPSSAPNAVRLAAQPDAFQNEIGWAYTCDI
                     SIPPEQMLADMQRMRDLGCTSLYIGHDNPARAGWGSAEPGLSFAVWYEIEMGGPEMAA
                     AEETLGVIIQGIEIARRVGLQVVLPIDYQMMQGRLWSQNNPDELAIGPDGLPLESHGD
                     NLASPYSEVFRRDKRSYYAWVQARLLSVYPHIAAINLGDEPSGSDYSPHAMAVFEERY
                     GVPWEDASSWQRGEFQAGVVADYAIWSAWQWNLINPDIRAMFTLHIERSRPFFPCYER
                     IYRQAPPGFIISADTHLHDAPEWVPLTWQDGNLLHNLARQLGVWSLSTGKDTMPWHSV
                     NHWGLNSGGIPEALENHDIIVNQTMDAGGRIAMTLAWAWNVRWQGLFRDEGKFAHVDK
                     EAMLSAISAAMTAAQPTLSEQREPEVHTVLYFPSKQLYQLVDEDRPAFTFEQAPWFDI
                     TEYDFTAKRYVTMTDGPALEEASRRGWPIVQVGGGDEAEPKESINYLPPDVREIHQGT
                     LWLGQTTWAATQAINRLMTEYEEARLHWSSEVGLYKGSIFANGQQGRIYEYVVFEREV
                     FHAVGDRFESTLIFRDLANGQFELGEGNRRFLAFPEPVIERLATTPIIGDAGSLAPSF
                     TTLRPLLTLNGELLDDDRTGEVADESITADGTRGTYADSTVAQITYAMFDNGHNIPNV
                     LFNEIVRRYGVETSDWWPRIGRPIGQALWVQTMIGAEGLRWVLTQPFERGILTYHPHY
                     VDRPEFVIQRALIGNLFLRLLTDSDEPYRPEYHDRFASSS"
     gene            2830..>3755
                     /locus_tag="VFV93_17535"
     CDS             2830..>3755
                     /locus_tag="VFV93_17535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5167213.1"
                     /translation="MIAAVRVRERARGMFIGWWIVAAAAGIQVLQTALLMQGYGAYIA
                     VLRDEFGWNKTSLSAAASLQRVESGLLGPIQGWLLARFGPRTIMRIGLVILAGGFFFF
                     SQVNSLPVFYLAFFTMAVGASLSGFMSVTTVVVQWFERRRATAISLMQTGMSIGGILV
                     PLVAWSLVALGWRTTAMLSGVIVLVVGLPLVQLMRPDPESYGLLPDGERPAERVSDDV
                     RAAPLVERVNFTPRQALRTRAFWCVGFGHALAVLVVSAIQIHLVVYLNEDQGFSVTGA
                     AVIVSIMTAITMVGQIGGGFLGDRFEKRKIAM"
BASE COUNT          569 a         1219 c         1278 g          689 t
ORIGIN      
        1 agcctcgccc ttgtccgttg cgatccacac agggcgaggc tgcggccatc gcccctacgc
       61 gagatgattc ccgccgatgt cagctcgatg acgcgaacct gtcgtggtat tccggccgat
      121 acggttcgtc gctgtcggtc aggaggcgga ggaagaggtt gccgatcagg gcgcgctgga
      181 tgacgaattc ggggcggtcg acgtagtgcg ggtggtaggt caggatgccg cgctcgaagg
      241 gctgcgtcag cacccagcgc aggccttcgg cgccgatcat cgtctggacc cagagcgcct
      301 ggccaattgg ccggccgatg cggggccacc agtcgctggt ctcgacgccg tagcgacgga
      361 cgatctcgtt aaagagcacg tttgggatgt tgtgcccgtt gtcgaacatc gcgtaggtga
      421 tctgggcgac cgtggagtcg gcgtaggtgc ctcgcgtgcc gtcggcggtg atggattcgt
      481 cggcgacctc gccggtcctg tcgtcgtcca gcaactcgcc gttcagcgtc agcagcgggc
      541 ggagcgtcgt gaacgatggc gccagcgatc cggcgtcgcc gatgatcggc gtcgttgcca
      601 gccgctcgat gaccggctcg gggaaagcga gaaaccggcg gttcccttcg cccagctcaa
      661 actgaccgtt ggccaggtcg cggaagatca gcgtcgactc gaagcgatcg ccgacggcgt
      721 ggaagacctc ccgctcgaag acgacatact cgtagatgcg cccctgctgg ccgttcgcga
      781 agatcgaccc cttgtagagc ccaacctcgc tgctccagtg cagcctcgcc tcctcgtatt
      841 cggtcatcaa gcggttgatg gcctgagtcg ccgcccaggt cgtctggccg agccagagcg
      901 tcccctggtg gatctcgcgg acatcgggcg gcaggtaatt gatgctctcc ttcggctcgg
      961 cttcatcgcc gccgccgacc tggacgatcg gccagccgcg ccggctggct tcctccagcg
     1021 ccgggccgtc cgtcatcgtc acgtagcgtt tggccgtgaa gtcgtactcg gtgatgtcga
     1081 accacggcgc ctgctcgaag gtgaaggcgg gccgatcctc gtcaacgagc tggtacagct
     1141 gcttcgacgg gaagtagagg acggtgtgca cctccggctc gcgctgttct gagagcgtcg
     1201 gctgggcggc ggtcatcgcg gccgagatcg cggacagcat cgcttccttg tcgacgtggg
     1261 cgaacttgcc ttcgtcgcgg aagagcccct gccagcggac gttccaggcc caggccagcg
     1321 tcatcgcgat ccgcccgccg gcgtccatcg tctggttgac gatgatgtcg tggttctcca
     1381 gcgcctccgg gatgccgccg ctgttcagcc cccagtggtt gacgctgtgc cagggcatcg
     1441 tgtccttgcc ggtgctcagc gaccagacgc cgagctggcg ggcgaggttg tggagcaggt
     1501 tgccatcctg ccaggtgagc ggcacccact ccggcgcatc gtgcaggtgc gtgtcggccg
     1561 agatgatgaa gccgggcggc gcctgccggt agatgcgctc atagcagggg aagaacggtc
     1621 gcgagcgctc gatgtgcagc gtgaacatgg cccggatatc ggggttgatc aggttccact
     1681 gccaggcgct ccagatcgcg tagtccgcga cgacgccggc ctgaaactca ccccgctgcc
     1741 aactgctggc gtcctcccac gggacaccgt aacgctcctc gaagaccgcc atcgcgtgcg
     1801 gcgagtagtc ggagccggac ggctcgtcgc cgagattgat ggccgcgata tgcgggtaga
     1861 cgctcagcag ccgtgcctgg acccaggcgt agtagctgcg cttgtcgcgc cggaagactt
     1921 ccgagtacgg cgaggcgaga ttgtcgccgt gtgactccag cggcaggcca tccggtccga
     1981 tcgccagctc atcagggttg ttctgcgacc agagccgccc ctgcatcatc tggtagtcga
     2041 tcggcaggac aacctgcaac ccgacccggc gggcgatctc gatcccctgg atgatgacgc
     2101 cgagcgtctc ctcggcggcg gccatctccg ggccgcccat ctcgatctca taccagaccg
     2161 cgaacgacag ccctggctcg gccgaccccc agccggcgcg ggccgggttg tcgtggccga
     2221 tgtagagcga tgtgcagccc aggtcgcgca tgcgctgcat gtcggccagc atctgctcgg
     2281 gcgggatcga gatgtcgcag gtgtaggccc agccgatctc gttctggaac gcgtccggct
     2341 gggccgcgag ccggacggcg ttgggcgcgg agctcggcgc tgtcttcgcc ggctcgctcg
     2401 gcttgtcgcc ggtcattgcg ctcgtgacgg gttcggtcag ccggcccagc gtgtccaggt
     2461 cggattccgg gtcgttgcgg accaggatcg acgctgccac gccagcggcc gagagggcgg
     2521 ccgaggcgag caactggcgg cgggtgatgc gcggcttggc cagccgcgca tgctccgatg
     2581 gacggaaact cgtcatgctc gcgtaaacat tcccagacag gcactcccaa ccggagctac
     2641 cgctcgaccg tagctgacga tgcgacacaa gatgaaagca cagattgcat cacgggtgat
     2701 cacacccagt ttgcacggac tctacacgat tcaacgcaaa cagttccaat ccggtagtcg
     2761 cggccggttg gcgtccgcgt gctaccatga tccgcgagag aacgcgccgg gggagtgagc
     2821 aggagcgttg tgatagcggc tgtgcgtgtc cgggagcggg cgcgcggcat gttcattggc
     2881 tggtggatcg tcgcggcggc ggccgggatc caggtgctac agacggccct gttgatgcag
     2941 ggctacgggg cctacattgc cgtcctgcgg gacgagttcg gctggaacaa gacatcgctg
     3001 tcggcggctg cctcgctgca acgcgtcgag agcggcctgc tcggccccat ccagggctgg
     3061 ctgttggccc gcttcggacc gcgaaccatc atgcggatcg gactcgtcat cctcgcgggc
     3121 ggtttcttct tcttctcgca ggtcaactcg ctgccggtct tctacctggc cttcttcacg
     3181 atggcagtcg gcgccagcct gagcggcttc atgtcggtca cgaccgtcgt cgtccagtgg
     3241 ttcgagcgtc gccgggccac ggcgatctca ttgatgcaga ccgggatgag catcggcggt
     3301 atcctcgtcc cgctggtcgc ctggtcgctg gtcgcgctcg gctggcggac gacggccatg
     3361 ctctcgggcg tgatcgtcct cgtcgtcggc ctgccgctcg tccagttgat gcgccccgat
     3421 ccggaatcgt acggtctgct gccggacggc gagcgcccgg ccgaacgcgt cagcgacgat
     3481 gttcgggccg cgccgctcgt cgagcgcgtc aacttcaccc cgcgccaggc gctgcgaacg
     3541 cgcgccttct ggtgcgtcgg cttcggccat gcgctggccg tcctggtcgt ctcggccatc
     3601 cagattcacc tggtcgttta cctcaatgaa gaccagggct tctcagtgac cggcgcggcc
     3661 gtcatcgtct cgattatgac ggccatcacg atggttggcc agatcggcgg cggctttctc
     3721 ggcgaccgct tcgagaagcg caagatcgcg atgat
//
LOCUS       DASUGJ010000776         4973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1796149, whole genome shotgun sequence.
ACCESSION   DASUGJ010000776 DASUGJ010000000
VERSION     DASUGJ010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796149"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /gene="serS"
                     /locus_tag="VFV93_17540"
     CDS             complement(<1..971)
                     /gene="serS"
                     /locus_tag="VFV93_17540"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX5167214.1"
                     /translation="MLDLRLIREKPDFVKAEIAKLNTTAPIDEILALDERRRTMLTEV
                     ESLKALLNAGSRETGRKAAGAERDAHIAAMRALGDEIAQLDAAAGEVDRELHAVLLTV
                     PNLPSPDAPVGKDESDNVVTASWGEQRRFDFMPKPHWEIMEGLGIIDFERGVKVSGSR
                     FYFLKGLGARLQRALITWMLDLRIEQGYEEVYPPYIVREEALVGTGNLPKFADTLFKD
                     REEGMWMIPTAEVPVTNMYRDEIIDGATLPINHCAYSACFRNEKASAGRDVRGIKRGF
                     QFDKIEMVKFVAPEESASEHEKLIADAAETLKRLEIPFRHLEICTGDL"
     gene            complement(1192..1779)
                     /locus_tag="VFV93_17545"
     CDS             complement(1192..1779)
                     /locus_tag="VFV93_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5167215.1"
                     /translation="MTEPLEPHQRTTIHRPVLVLNQNYEPLNVCNMRRAMGLVFGEKA
                     EVLEVYPAPLASASRSYDAPSVIRLIYMIRRPRPRVKLTRREIFIRDDFTCQYCGIRG
                     GDLTIDHITPRSRGGQHTWENLVSACRSCNHRKGGKTVIEARMRLRRDPYEPKAGLYY
                     TISRRLNAAAHENWSKFIPGFEGLQPTTSISIASD"
     gene            complement(2039..4918)
                     /gene="uvrA"
                     /locus_tag="VFV93_17550"
     CDS             complement(2039..4918)
                     /gene="uvrA"
                     /locus_tag="VFV93_17550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5167216.1"
                     /translation="MPIDRIVVRGAREHNLKNIDVEIPRDKLVVLTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQVLGQMEKPDVDQIDGLSPAISIDQKGSSRNPRSTVGT
                     VTEIYDHLRLLYARIGHPHCPVCGREISRQTPQQMADIVLGFEDGSRILILGPVIRDR
                     KGEHQGVFEQIRKSGFVRVRVDGELRELEEEIKLAKTKKHTIEVVVDRLVVRHETPED
                     IDALRIRLIDSLETALKLGGGYVSVQVVDGPETVFSENYACPLGHGSIGEIEPRSFSF
                     NSPHGACPACTGLGIAMEFDPEMVVSDSSLTLAGGAITPWSRPGREGNVWYEAMLAAV
                     AERFEIPMDIPFRNLTQEQRDIILHGTGSPTVTVRYTSRSGRRRQYTVKYEGVIPHLT
                     RRLEQTASDYIREEIQQYMSSRPCPVCKGARLKPEILAITVADRSIVEITTMSVVEAL
                     RFAEELQDGSLSDREFLIARQVLKEIRERLGFLVDVGLEYLTLDRAAATLSGGEAQRI
                     RLATQIGSRLMGVMYVLDEPSIGLHPRDNARLIHTLVSLRDIGNTLLVVEHDEETMRA
                     ADWIIDIGPGAGEHGGYVVAAGTFDDITDHPDSITGQYLSGRRVIPVPDTRRTGNGKQ
                     LVLKGASENNLKNLTVEFPLGTFICVTGVSGSGKSTLITDTLYKRLAQILYRAHDKPG
                     KFSALEGVEHIDKVIDIDQSPIGRTPRSNPATYTGAFTHIRELYAAMPEAKARGYKAG
                     RFSFNVKGGRCEACSGEGIVKIEMNFLPDVYVPCEVCHGKRYNREALEILYKGKSIAD
                     VLDMTVAEGLEVFENIPKIANKLRTLNDVGLGYIHLGQPATQLSGGEAQRVKLATELS
                     RRATGKTMYILDEPTTGLHFADIERLLGVLQRLTDAGNTILVIEHNLDVIKSADWIID
                     LGPEGGHRGGEIIATGTPEEIADNDRSYTGHALRALSRELALAAD"
BASE COUNT          800 a         1500 c         1709 g          964 t
ORIGIN      
        1 aggtcgccgg tgcagatctc cagatgccgg aaggggatct ccagccgctt gagcgtctcg
       61 gcggcgtcgg cgatcagctt ctcgtgctcg gaggccgatt cctcgggggc gacgaacttg
      121 accatctcga tcttgtcgaa ctggaagccg cgcttgatgc cgcgcacgtc gcgaccggcg
      181 ctggctttct cgttgcggaa acaggccgag tacgcgcagt ggttaatcgg gagtgtcgcg
      241 ccgtcgatga tctcatcgcg gtacatgttg gtgacgggca cctcggcggt cgggatcatc
      301 cacatgccct cctcgcgatc cttgaagagc gtgtcggcga acttgggcag gttgccggta
      361 ccgaccagcg cctcctcgcg gacgatgtag ggtgggtaga cctcctcgta gccctgctcg
      421 atgcgcaggt cgagcatcca ggtgatcagt gcccgttgga ggcgggcgcc gagtcccttc
      481 aggaagtaga agcgcgagcc ggagaccttg acgccccgct cgaagtcgat gatgccgagg
      541 ccctccatga tctcccagtg cggcttcggc atgaagtcga agcggcgttg ctcgccccag
      601 ctggcggtaa cgacgttgtc cgactcgtcc ttaccgaccg gtgcatccgg agagggcagg
      661 tttgggacag ttagaaggac ggcgtgcagt tcgcggtcga cctcgccggc ggcggcgtcg
      721 agttgggcga tctcgtcacc gagcgcccgc atcgcggcga tatgggcgtc gcgttctgcg
      781 ccggccgcct tcctgccggt ctctcgggaa ccggcgttga gcagggcctt gagcgattcg
      841 acctcggtca gcatcgtccg gcggcgctcg tcgagcgcca ggatctcgtc gatcggcgcg
      901 gttgtgttca gcttggcgat ttctgccttc acgaaatcgg gcttctcgcg gatcaggcgt
      961 aagtccagca ttgttcgtct ccttcgtctg tgtcccgttc agtgtacctt cgtgctgcac
     1021 aggcagcgcc cggtactggc ggagacgcca ccacaggttc ggagaagcgg gtcgtcgtgt
     1081 tgacattcga gtcgcataca ctgatgtctt tcttgatcgt gcgtgcatga aaaacgccct
     1141 accggtgtag ggcgtcgcga tcgactgcgg gcgggcagcg gcagccgatc gctagtcact
     1201 cgcgatactg atggaggtgg tcggttgtag cccttcgaag ccggggatga acttggacca
     1261 gttctcgtgg gcggccgcgt tgagccggcg tgagatcgtg tagtagagcc cggccttcgg
     1321 ttcgtaggga tcgcgacgca ggcgcatccg cgcctcgatc accgtcttgc cgcccttgcg
     1381 gtggttacag ctgcgacagg ccgagacgag gttctcccag gtgtgctggc cgccgcgaga
     1441 tcgtggcgtg atgtggtcga tcgtcaagtc gccaccccgg atgccgcagt actggcaggt
     1501 gaaatcgtcg cggatgaaga tctcgcgacg ggtcagcttc acgcgcgggc gcggccggcg
     1561 gatcatgtag atcagccgga tcaccgatgg cgcgtcatac gaacgggacg cgctcgccag
     1621 tggcgcggga tagacttcca gcacctcggc cttctcgcca aacacgagac ccattgcgcg
     1681 tcgcatgttg cagacattga gcggctcgta gttctgattc aggaccagaa ccgggcgatg
     1741 gatggtggta cgttggtgag gctccaatgg ctcagtcatg gtgcgtttcc aggccgctct
     1801 cgcactgagc gtgcatagct ctgacggcgg aatcctgacg cctgactcat catactaagc
     1861 gcgcggccac cttcgcaagc acgggcatgg gatatcggac actgtgttcg tacgaggggt
     1921 ccagccgctc ggaagcagag gccggtcttg taccggcctc gctctccccc ccaacgcggc
     1981 ctgaccgaag catggccggc acaggaccgg cctctgcttt tgggcgttga gagggttctc
     2041 agtcggcggc cagcgccagt tcgcgactga gtgcgcgaag ggcgtggccg gtgtaggagc
     2101 ggtcgttgtc ggcaatctct tcaggagtgc ccgtcgcgat gatctcgccg ccgcgatggc
     2161 cgccttccgg cccgaggtcg atgatccagt cggccgattt gatgacgtcc aggttgtgct
     2221 cgatgacgag gatcgtgttg ccggcgtcgg ttagccgctg gaggacgccg agcagccgct
     2281 cgatatcggc gaagtggaga ccggtcgtcg gctcgtcgag gatgtacatc gtcttgccgg
     2341 tcgcccgtcg actcagttcg gtcgccagct tgacgcgctg ggcttcgccg ccggagagct
     2401 gggtggccgg ctgaccgagg tggatgtagc cgagcccgac gtcgttgagc gtgcgcagct
     2461 tgttggcgat cttggggatg ttctcgaaga cctccagccc ctcggcgacg gtcatgtcga
     2521 gcacgtcggc gatcgacttg cccttgtaga ggatctccag cgcctcacgg ttgtaacgct
     2581 tgccgtggca gacctcgcag gggacgtaga catccggcag gaagttcatc tcgatcttga
     2641 cgataccctc gccggagcag gcctcgcagc ggccaccctt gacgttgaac gagaagcggc
     2701 cggccttgta gccacgggcc ttcgcctccg gcatcgccgc gtacaattcg cggatgtggg
     2761 tgaacgcgcc ggtgtaggtg gccgggttgg aacgcggcgt tctgccgatc gggctctggt
     2821 cgatgtcgat gaccttgtcg atgtgctcga cgccttcgag tgcgctgaac ttgcccggct
     2881 tgtcgtgagc gcgatagagg atctgggcca ggcgcttgta aagcgtgtcg gtgatgagcg
     2941 tgctcttgcc ggagccggag acaccggtga cgcagatgaa cgtgccaagc gggaactcga
     3001 ccgtcaggtt cttgaggttg ttctcgctgg cgcccttcag gacgagctgt ttgccgtttc
     3061 cggtccgacg cgtgtccggg actggaatga cccgccgtcc gctcagatac tggccggtga
     3121 tcgaatcggg atggtctgtg atgtcatcga aggtgccggc cgcgacgacg tagccgccgt
     3181 gttcaccggc gccggggccg atgtcgatga tccagtctgc cgcccgcatt gtctcctcat
     3241 cgtgctcgac gaccagcaac gtattgccga tgtcgcgcag cgagacgagc gtatggatca
     3301 ggcgggcgtt gtcgcgcggg tgaagcccga tcgacggctc gtcgaggacg tacatgacgc
     3361 ccatcagccg gctgccgatc tgggtcgcca ggcggatgcg ctgggcttcg ccgccagaga
     3421 gcgtcgccgc cgcccggtcg agcgtcagat actcaaggcc gacatcgacg aggaagccga
     3481 gccgctcgcg aatctccttg agcacctggc gggcgatcag gaactcgcgg tccgagagac
     3541 tgccgtcctg aagctcctcg gcaaaacgca gcgcctcgac gaccgacatc gtcgtgatct
     3601 cgacgatcga tcgatcggcg accgtgatcg ccaggatctc cggcttgagc cgcgctccct
     3661 tgcagaccgg gcaggggcgc gacgacatgt actgctggat ctcctcgcgg atgtagtcgg
     3721 acgcggtctg ctccagccgg cgggtgagat gcgggatgac gccctcgtac ttgaccgtat
     3781 actgccgtcg ccggccgctg cgtgacgtgt agcgaacggt gacggtcggc gatccggtgc
     3841 cgtgcagaat gatgtcgcgc tgctcctggg tcagattgcg gaacgggatg tccatcggga
     3901 tctcgaagcg ctcggcgacg gcagccagca tcgcctcgta ccagacgttg ccctcgcgac
     3961 ccggacgcga ccagggcgtg atcgcgccgc cggccagcgt cagcgacgag tcggagacga
     4021 ccatctcggg gtcgaactcc atcgcgatcc cgaggccggt gcaggctggg caggcgccat
     4081 gcgggctgtt gaacgagaag gagcgtggct cgatctcgcc gatcgagccg tggccaagcg
     4141 ggcaggcgta gttctcggag aagacggtct cgggaccgtc gacgacctgg accgagacat
     4201 agccgccgcc gagcttgagt gctgtctcga gcgagtcgat caggcgaata cgaagtgcgt
     4261 cgatgtcctc cggcgtctcg tgacggacga cgagccggtc gacgacgacc tcgatcgtgt
     4321 gcttcttcgt tttggccagc ttgatctctt cctcaagctc gcgcaactcg ccatcgacgc
     4381 ggacgcggac gaagcctgac ttgcggatct gctcgaagac gccctgatgc tcgcccttgc
     4441 ggtcgcggat gaccggcccg aggatgagga ttcgcgagcc gtcttcgaac cccaggacga
     4501 tgtcggccat ctgctgcggg gtctggcgcg agatctcgcg accgcagacc gggcaatgcg
     4561 gatggccaat ccgggcgtag agtagccgca aatggtcgta gatctcggtg acggtgccga
     4621 cggtcgatcg cgggttgcgg ctggagccct tctggtcgat cgagatggcc ggcgagagtc
     4681 cgtcgatctg gtcgacgtcg ggcttttcca tctggccgag gacctggcgg gcgtaagcgg
     4741 atagcgattc gacgtagcgg cgctggccct cggcgtagat cgtgtcgaac gcaagcgacg
     4801 acttacctga gccggagaga ccggtcagga caaccagctt gtcgcgtggg atctcgacat
     4861 caatgttctt gaggttgtgc tcgcgggcgc cacggacgac gattcgatcg atgggcatct
     4921 cgtctccggt ctttgcaaac gtggtccatc agcggggtta cggctggtgt ccg
//
LOCUS       DASUGJ010000777         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_683960, whole genome shotgun sequence.
ACCESSION   DASUGJ010000777 DASUGJ010000000
VERSION     DASUGJ010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683960"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFV93_17555"
     CDS             <1..481
                     /locus_tag="VFV93_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167217.1"
                     /translation="TVAGLTGGKKALTIFPEATIEIAPSGQGYRVRWTITLDHRGDPN
                     GNPVYNGYEYAWLSAYLPEGARLVSTSRTPSSGDLAGDPRSRSFGIGIMPGTTVQTTL
                     DFEMPGTDQILLRRQPGFNDLAVRITGTTASCPIDWSLTLTEDMLVDLDTCVAVPAE"
     gene            complement(696..1757)
                     /locus_tag="VFV93_17560"
     CDS             complement(696..1757)
                     /locus_tag="VFV93_17560"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEX5167218.1"
                     /translation="MPIETVELLRAVTSAFGPSGFEDEVRDLIREMVEPLVDEVRVDT
                     LGNLLVTRTGRGETTFMLDAHMDEIGFIISWIEEGGFLRFQPIGGWDARIIPSHALTI
                     LTDNGTKIKGAIGTPPPHVLRPEDREKPFKIEDLFVDIGVDSADEARALGVRIGSPAV
                     VSYPFEQLGEQRVMAKALDDRAGCAVMIRTLEALKDVDLDVTFVAAFTVQEEVGLRGA
                     GTAAWQIDPDIAIALEGTIAADVPGVPAPRQPTRQGQGPSIRIMDSGMIGMPRMIRAL
                     AETAESADIPFQYQVPAPGGTDAGAIHRSRGGVLSGAVSVPCRYIHAPCTVMRIDDFE
                     NAARLVTEFVRRCPGVVGM"
     gene            1961..3148
                     /locus_tag="VFV93_17565"
     CDS             1961..3148
                     /locus_tag="VFV93_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5167219.1"
                     /translation="MADINQVRDMLPGVTDTVYLNTGTCGPLPTIAYEAMEAELKTDL
                     TKARIDQSHFPKIGESRMAVREAVAAVVKASPSEIAITSSTTDGMYVAIMGYRWQPGD
                     ELLLTNIEHPGGMIPSFLAKRRFGVRVKVVDIGLGGGYPASIVSAFERAITPRTRMIV
                     LSHVSYTTGAKLPLAEIIEMAHAHDVLVVADAAQSYGPLDLDLPAIGVDAYACSGQKW
                     MCGPDGTGALYVRSASIGEFEQTFVSGGLIRETMDYYSATYAPAFGAARFDTAGRNVA
                     LLIGQATSTRWIGQELGMTWVAERVSQVAGYTYDQLSNLKGVTIVTPREALAGLIAFN
                     VDGITGPDLTKRLAEEHNVTIRFVTKYINNPDAARVSLGFYNTEEDVDRFIAGVTAIQ
                     GTL"
     gene            3150..>3427
                     /locus_tag="VFV93_17570"
     CDS             3150..>3427
                     /locus_tag="VFV93_17570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5167220.1"
                     /translation="MEKTPRTVDDLNAWSPGTLPGLLGIRITELVAGGVTGEMPVRPD
                     LLAPNGYLHAASVVGLADTVSGYGCRANLPDGAIGFTTIELKSNFLGTA"
BASE COUNT          574 a         1122 c         1139 g          592 t
ORIGIN      
        1 gacggtcgcc ggcctgaccg gcggcaagaa agcgctgacg atcttcccgg aggcgacgat
       61 cgagattgcg ccgtcgggcc agggctatcg cgtccgttgg acgatcacgc tcgatcaccg
      121 tggcgacccc aacggcaacc cggtctacaa tggttatgag tacgcgtggc tctcggcgta
      181 cctcccggag ggagcacggc tcgtctcgac cagccgcaca ccctccagcg gcgatctggc
      241 cggcgaccca cggtcaagga gcttcggcat cggcatcatg ccggggacaa ccgtgcagac
      301 gacgctcgac ttcgagatgc ctgggaccga ccagatcctg ttgcgccgcc agcccggctt
      361 caacgacctc gccgttcgca tcactggcac gaccgcgtcg tgcccgatcg actggtcgct
      421 gacgctcacc gaggacatgc tcgtcgatct ggacacgtgc gttgcagttc ccgccgaatg
      481 acgattgcta ctccgtgcag gggccgatcc tgtatcggcc cgcgccgtga ggcgcccgtt
      541 cagcgatatg cgtggttgat ccaagggcgc aggcgttagg gatctgcgta aaacgcggac
      601 gtcgtcaagc gtgcgcctca cggcgcgggc cgatacagga tcggcccctc cacgggattg
      661 ggatcgcgtg ggcatgctcc agcgacatca gttccttaca tgccaacgac gcctgggcag
      721 cggcggacga actcggtgac gaggcgggcg gcgttctcga agtcgtcgat ccgcatgacg
      781 gtgcagggcg cgtggatgta gcggcaggga accgagaccg cgccggagag gacgccgccg
      841 cgcgagcgat ggatcgctcc ggcgtcggtt ccacccgggg ccggcacctg atactggaac
      901 gggatatcgg ctgattcggc ggtttcggcc aacgcccgga tcatgcgtgg catgccgatc
      961 atgccgctgt ccatgatgcg aatcgatgga ccctgcccct gtcgcgttgg ctggcgcggg
     1021 gcggggacgc cgggcacatc ggcggcgatc gtcccttcga gggcgatggc gatgtccggg
     1081 tcgatctgcc aggcagcggt tccggcgcca cgcagcccga cctcctcctg gacggtgaag
     1141 gctgcgacga aggtgacgtc gaggtcgacg tccttgaggg cttcgagcgt gcggatcatg
     1201 acggcacagc cggcccggtc gtcgagcgcc ttggccatca cgcgttgctc gccgagctgc
     1261 tcgaacgggt agctgacgac ggctggcgag ccgatccgga caccgagcgc gcgggcctcg
     1321 tcggcgctgt cgacgccgat gtcgacgaac agatcctcga ttttgaacgg cttctcgcgg
     1381 tcttcgggcc gcagcacgtg cggcgggggc gtcccgatcg cgcctttgat cttcgtgccg
     1441 ttgtcagtca ggatcgtcag cgcgtgcgag gggatgattc gcgcatccca gccgccgatc
     1501 ggctggaagc gtaggaagcc gccctcctcg atccagctga tgatgaagcc gatctcgtcc
     1561 atgtgtgcgt cgagcatgaa ggtcgtctcg ccgcgccccg tgcgggtgac gagcaggttg
     1621 ccgagcgtat cgacccgcac ttcgtcgacc agcggctcga ccatctcgcg aatgagatcg
     1681 cggacctcgt cctcgaagcc ggacggcccg aacgcggatg tcaccgctcg caacagctcg
     1741 acagtctcaa tcggcatcgg tttctgcccc cttcgtcgtt gcgtgttcat tgagtggtct
     1801 tgtaccacga tcgtgcactt gccgcgccga tcgcccttga cgaatcatct ccgacgcgac
     1861 atgctgcccg gcgtctaccg ttcgccgaag cagacgacgt tcgcctgtct ggcggacttt
     1921 cgatattgtt gcgtcgaggg tatcaggagg agtgcaccgg atggccgata tcaaccaggt
     1981 acgcgacatg ttgccgggcg tcaccgacac ggtctatttg aacaccggca catgcggacc
     2041 gctgccgacg atcgcctacg aggcgatgga ggcggaactg aagaccgacc tgaccaaggc
     2101 gcggatcgac cagagccatt tcccgaagat cggtgaatcg aggatggccg tgcgtgaggc
     2161 cgtcgctgcc gtcgtcaagg ccagtccgag tgagatcgcg attacctcca gcacgactga
     2221 cgggatgtac gtcgcgatca tgggctaccg ctggcagccg ggcgacgagc tactgctgac
     2281 caacatcgag catccgggcg ggatgattcc gtcgttcctc gccaagcggc gcttcggggt
     2341 gcgggtcaag gtcgtcgata ttggcctcgg cggtggctat ccggcctcaa tcgtcagcgc
     2401 cttcgagcgg gcgatcacgc cacggacgcg gatgatcgtc ctctcgcacg tctcctacac
     2461 gaccggcgcg aagctgccgc tggcggagat catcgagatg gctcacgcac acgatgtcct
     2521 cgtcgtcgcc gacgccgccc agtcgtacgg gccgctcgac ctcgacctgc ctgccatcgg
     2581 cgtcgacgcc tacgcctgct ccggccagaa gtggatgtgc gggccggatg gcaccggcgc
     2641 gctctacgtc cgctcggcca gcatcggcga gttcgagcag acgtttgtct cgggcggcct
     2701 gatccgcgag acgatggact actacagcgc cacctacgcg ccggcgttcg gcgcagcccg
     2761 cttcgacacg gccggtcgca acgtcgcgct gctgatcggg caggcaacct cgacgcgctg
     2821 gatcggccag gaactgggca tgacctgggt cgcggagcgg gtcagccagg tcgccggcta
     2881 cacctatgac cagctatcga acctgaaggg ggttacgatc gtgacgcccc gcgaggcgct
     2941 ggccggcctg atcgcgttca acgtcgacgg gatcaccggt cctgacctga cgaagcggct
     3001 ggccgaggag cacaacgtca cgatccgctt cgtgaccaag tacatcaaca acccggacgc
     3061 cgcccgtgtc tcgctcggct tctacaacac cgaagaggat gtcgatcgct tcatcgccgg
     3121 tgtgaccgcc atccagggca cactctagga tggagaagac gccgcggacg gttgacgacc
     3181 tcaatgcctg gtcacccggt acgctaccgg gactgctcgg catccgcatc acggagcttg
     3241 ttgcaggggg tgtgacgggc gagatgccgg ttcgccccga tctgctggcg ccgaacggtt
     3301 acctgcacgc cgcctcggtc gtcgggctgg ccgataccgt ctccggctac ggttgccgcg
     3361 cgaacctgcc ggacggggcg atcgggttca cgacgatcga gctgaagtcc aacttccttg
     3421 ggacggc
//
LOCUS       DASUGJ010000778         7098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_126917, whole genome shotgun sequence.
ACCESSION   DASUGJ010000778 DASUGJ010000000
VERSION     DASUGJ010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126917"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VFV93_17575"
     CDS             <1..1156
                     /locus_tag="VFV93_17575"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5167221.1"
                     /translation="ERRRGAAVGQLIVGRPAPKQLFTVSAPLEMTSAISLTFRAASAA
                     ESDIADPGFDRWLVDAWRSLDAGMRHDLELLLGFSGRLLYYIEEMLFAFDALSPDRLD
                     ASFDDYLAFLTSLPPARFQEMAANALVRIYRDRGLNETPPASDSPNEWRMFLRPGITR
                     ANIDEAAALVTSPDQLRLRTLSLLEGFWRQCYQPEFERNQADLQRSVRFAQALSHPSV
                     QVTFQELTGHRLPAEIEGWFDEVERVVFCPSSHVGSFIQYILYPPALILYFNAAAVTR
                     DRPAQARAARKDDAPLETPEVLDGLRAMSDPSRMRIIEMLRDGELYAQEIVAQLGISQ
                     SAVSRHLSTLESADLVTVRPANGMKYYAIDRARLRALSSHIESLADIGSR"
     gene            1211..2140
                     /locus_tag="VFV93_17580"
     CDS             1211..2140
                     /locus_tag="VFV93_17580"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5167222.1"
                     /translation="MAATETRWLTNDTAIIDLSFQSVPKVFAAYLLETSDGLALIETG
                     PTSTVAALEAGVEALGREMDDIRHLLVTHIHLDHAGAAGLLMQRNPQTRLFVHEIGAP
                     HVIDPTNLVLSATRIYGDRMDQLWGEVLPVPADRVVAVSDGGTIDLGDRRVSVIYTPG
                     HASHHVAFHDLRTNMTVVGDVAGIRIPPSPDVLPPTPPPDINVEHWHESVERLRKLAP
                     LRMLLSHFGVVNDVQDHLALLDRRLDEWTALIESFVADGLDRDTMADRLMAHVMGQMV
                     ADGTTSLAQQTALASPYGMSVDGLLRYLRKRGG"
     gene            complement(2244..3620)
                     /locus_tag="VFV93_17585"
     CDS             complement(2244..3620)
                     /locus_tag="VFV93_17585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX5167223.1"
                     /translation="MASALEDYRRAERYVNSLIMGPPSPPPGTPPEEIRARAVARLDR
                     LRAFLGYLGDPQRQYRTIHVAGTSGKGSTTTFTASILTAAGFKTGAHVSPYLQVATEK
                     LQIDGRIIPAGRYAKLVDDMRASLEAWVASRHERPNYGEFWVAMTYRYFAEEQVEVAV
                     IEVGAGGRFDVTNVIEPDVAAITSIGYDHIVTLGSTLPEIAWHKAGIIKPGSIAVSAV
                     GEPEAVPVIEAEARQQGAELRRVRPGQEYEDVTSNEHGTSFRDTATGKRFHVTLPGTF
                     QAANAATAMTITRAFNPERVSDDAITSGLSAARFPGRMETVQHDPLVLLDGAHNPEKV
                     ASLVRNLDILFPNRSRIFVFGALESKNYQEMLTSLLPQASRLIATAPYVLAKPSTTAA
                     EIAALAPAGLPTEAIDDALAAADRAIALAGPDDLVIITGSLYLVGNVRERWYPTERIL
                     EQGTCWPK"
     gene            3752..5167
                     /locus_tag="VFV93_17590"
     CDS             3752..5167
                     /locus_tag="VFV93_17590"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX5167224.1"
                     /translation="MTSLTHLARPPSINQHHILWRLLGTALVLAGLVLTGIAGAFAQG
                     SDAQPHVGLIDIDGTITPVMATYVGRGLDRSADRGDSAVILRMDTPGGLSSAMDDIVS
                     DILASPIPVIVYVAPEGARAASAGVYITYSAHVAAMAPATNIGSATPVNLGDESNDDN
                     QSTMDRKVINDAVAKIRGLAERRGRNADWAEAAVRDADNITASVAVEKNVVDFIAGDL
                     DSVLAQADGRSVVVRGQPVTVETAGASVSEIDMSFFEKLLQVVTDPNIAFVLVSLGTL
                     AIVFEIANPGGIGPGAVGGLMLVTGFYALGTLDTNWAGLALMGLAFMLFVIDVFVPTH
                     GILTITGIISFLFGGLLLSNTRNDDVLEISRVVIFTITALMGVFFLFLAGSAWRSHGR
                     PPSTGESTLIGAIAVVRQPLEPDGMVFIQGELWQATSTDGPVERGHFVRILGIDGLRL
                     TVTPVTVEAGVSPEALESAPG"
     gene            5231..6070
                     /locus_tag="VFV93_17595"
     CDS             5231..6070
                     /locus_tag="VFV93_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5167225.1"
                     /translation="MDNGVLIALGAIVLFILIVLSASIKIVQEYERGVIFRLGRLMGA
                     RGPGLILLIPFIERMMKVDLRTLTMDVPRQEVITRDNVTVNVNAVVYFRVVDPNAATV
                     NIADHVRATSQIAQTTLRSVLGQVELDQLLSERENINQRLQAIIDEQTEPWGVKVSVV
                     EVKDVELPQSMQRAMARQAEAEREKRAKVIHAEGEFQASQQLQEAAAIIAEEPIAIQL
                     RYLQTLTEISAEKNSTIIFPLPMDLIAAVVPGFQTSSQASAHETTTTPPAEPTPPQHE
                     EPA"
     gene            6070..6951
                     /locus_tag="VFV93_17600"
     CDS             6070..6951
                     /locus_tag="VFV93_17600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5167226.1"
                     /translation="MGRLPDAVRDWQAQLTPVTVDGVRSFVLDTGMGDPVVFLHGIPT
                     QAYLWRDVAQVVSHRWRVIAPDLLGFGFSDRPEDADYSPGGQASFIQRVLSELGVERY
                     ALVVHDYGALVGAELLTRDPDSVTNMVVLNTSFWEDDWSGSQLNPFSLLSLPGVGEVA
                     FRVARKFMLREAFGLYVSERQRLTSDVMDVYWHPFTDGFADVLLRLFRDRAGRMDEQA
                     FCRWRDAIEAFDRPSLVIWGDRDPTFRIWTGRRIAEILPAGNLELFEHSNHFVPEDRP
                     EALGRLIKVLLSGRYKG"
BASE COUNT         1171 a         2294 c         2326 g         1307 t
ORIGIN      
        1 agaacgacga aggggagcgg ctgtggggca gttgattgtc ggcaggcccg cgccaaaaca
       61 gctcttcaca gtctcggctc cgctcgaaat gacgtcggcc atctcgctga cgttccgagc
      121 cgccagtgct gctgagagcg acatcgctga tccggggttc gaccgctggc tggtcgatgc
      181 ctggcgttcg ctcgacgccg gtatgcgaca tgacctcgaa ctgctgctgg gtttctcagg
      241 ccggttgctc tactacatcg aggagatgct gttcgccttc gacgcgctca gccccgaccg
      301 gctcgacgcc agcttcgacg actaccttgc gttcctgaca tcgctgccgc cggccaggtt
      361 ccaggagatg gcagccaacg cgctggttcg catctaccgc gatcgtgggc tgaacgagac
      421 gccaccggcc agcgacagtc ccaacgagtg gcgcatgttt ctccggccgg gcatcacccg
      481 cgccaacatc gacgaggcgg cggcgctcgt gacctcgccc gatcaactca ggctgcgaac
      541 gctgtcgctg ctggagggct tctggcggca gtgctaccag ccggagttcg aacggaatca
      601 ggcagatctg caacgctcgg tgcgcttcgc ccaggcactc tcgcacccgt cggttcaagt
      661 gacgtttcag gagctgaccg gccaccggct gccggcggag atcgagggct ggttcgacga
      721 ggtcgaacgg gtcgtcttct gcccgtcgtc gcacgtcggc agcttcatcc agtatatcct
      781 ctacccgccg gcgctgattc tctatttcaa cgcagcagcc gtcacgcgcg atcgtccggc
      841 ccaggcgcgc gccgcccgca aggacgacgc gccgcttgag acgcccgagg tgctggacgg
      901 tctgcgggcg atgtcggacc cgtcgcgaat gcggatcatc gagatgctgc gcgacggtga
      961 actgtacgcg caggagatcg tcgcccagct tggcatcagc cagtcggccg tctcgcgcca
     1021 tctcagcacg ttggagtcgg ccgatctggt caccgtccgt cccgccaacg gcatgaagta
     1081 ctacgcgatc gaccgcgccc ggctgcgtgc gctgtcgagt cacatcgaat cgctggctga
     1141 catcggttcc cgctaggcac gttgcctctg tctcctcggc gccttcccag gaactcagaa
     1201 aggatggcac ttggctgcca ccgaaacccg ctggctgaca aacgacaccg ccatcatcga
     1261 cctgagcttt cagagcgttc caaaggtatt tgccgcgtat ctgctcgaaa cgagcgacgg
     1321 tctggccctg atcgagacag ggccgacgag caccgtcgcc gccctcgaag cgggcgtcga
     1381 ggcgctcgga cgtgagatgg acgatatccg gcacctgctc gtgacgcaca tccacctcga
     1441 tcacgccggc gcggccgggc tgctgatgca acgaaatccc cagacgcgcc tcttcgtcca
     1501 tgagatcggc gcaccccatg tgatcgatcc gaccaacctc gttctcagcg cgacccgtat
     1561 ctatggcgat cggatggacc agctctgggg ggaagtcctg ccggtcccgg ccgaccgcgt
     1621 tgtggccgtc tcggacggcg gcacgatcga cctcggcgat cgcagagttt ccgtcattta
     1681 cacgcccggc cacgcctccc atcacgtcgc atttcacgat ctgcgcacga acatgacagt
     1741 tgttggtgac gtggcgggca tccgcatacc accgtcgccg gatgtgctgc caccgacgcc
     1801 gccaccggac atcaacgtcg agcactggca cgagagcgtc gaacgcctgc gcaagctcgc
     1861 tccgctgcgg atgctgctgt cgcacttcgg cgtcgtcaac gatgtccagg accacctcgc
     1921 cctgctcgat cgtcgcctcg acgaatggac ggcgctgatc gagtcattcg tagccgatgg
     1981 tctcgaccgc gacacgatgg ccgaccgcct gatggcccac gtcatgggcc agatggtagc
     2041 cgacggcacg acctccctcg cccaacagac tgccctcgcc agcccatacg gaatgtccgt
     2101 cgacggcctg ctgcgctatc tgcggaagcg tggtgggtga tgcgggacgg gaaccagatg
     2161 aacggcgtca gcgagggccg acaccccttg ccctgtcatc attcgcttgc gccgttcatc
     2221 tggttcccgt cccgcgtccc gtgtcacttc ggccaacacg ttccctgttc gaggatgcgt
     2281 tcggtgggat accagcgttc gcggacgttg ccgaccaggt agagcgaacc ggtgatgatg
     2341 acgaggtcgt cggggccggc gagcgcgatg gcgcggtcgg cggcggccag cgcgtcgtcg
     2401 atggcttcgg tcggcaggcc ggctggggcg agagcggcga tctcggccgc cgtcgtggac
     2461 ggcttggcga gcacgtaggg cgcggtcgcg atcaggcggc tcgcctgcgg cagtagcgag
     2521 gtgagcatct cctggtagtt cttggattcc agcgcgccga agacgaagat gcgcgagcgg
     2581 ttcgggaaga ggatgtccag attgcggacc agcgaggcaa ccttttccgg gttgtgcgcc
     2641 ccgtcaagca ggacgagcgg gtcatgctga accgtctcca tccggccggg gaagcgtgcg
     2701 gcgctgaggc cggatgtgat ggcgtcatcg ctgacacgct cggggttgaa cgcgcgggtg
     2761 atggtcatgg ccgtcgcagc gttggcggcc tggaacgtgc ccggcagcgt cacgtgaaag
     2821 cgcttgccgg tggcggtgtc acggaacgac gtcccgtgct cgttgctggt gacgtcttcg
     2881 tattcctggc cgggccggac gcggcgtagc tccgcgccct gctggcgcgc ttctgcctcg
     2941 atgaccggca cagcctccgg ctcgccaacc gccgagacgg cgatcgagcc gggcttgatg
     3001 atgcccgcct tgtgccaggc gatctccggc aatgtcgagc cgagcgtgac gatgtggtcg
     3061 tagccgatgc tggtgatggc tgccacgtcc ggttcgatga cgttggtgac gtcgaagcgc
     3121 ccaccagcgc cgacctcgat gacggcgact tcgacctgtt cctcggcgaa gtagcgatac
     3181 gtcatcgcga cccagaactc gccgtagttc ggccgttcgt ggcggctggc gacccacgct
     3241 tccagactgg cgcgcatgtc gtcgaccagc ttcgcgtagc ggccggccgg gatgatgcgg
     3301 ccgtcgatct ggagcttctc ggtcgcgact tgcaggtatg gagagacatg ggcgccggtc
     3361 ttgaacccgg ctgccgtcag gatgctggcc gtgaacgtcg tcgtcgagcc cttgccggat
     3421 gtcccggcga cgtggatcgt cctgtattgc cgctgcgggt cgccgagata gcccaggaag
     3481 gcgcgcaagc ggtcgagacg ggcgacggcg cgggcgcgga tctcctcggg cggcgtcccg
     3541 ggcggcggcg acggcggccc catgatgaga ctgttcacat atcgctctgc gcgccgatag
     3601 tcttccagtg ccgaagccat gctgtccctg ccttgcccgt cgatcaccgt gccgccccaa
     3661 cggcgtcgaa attgtaccgg gagtgcttcc cggcgccgcg cgttgtcagg ctggctatac
     3721 ttgaaccggg taggggttaa ggagtgaagt catgacctcg ctgacccatt tagcgcgtcc
     3781 tcccagtatc aaccagcatc atatcctctg gcgactgctc gggacggccc tcgtgctggc
     3841 tggtttggta ctgaccggta tcgcgggcgc tttcgcgcag gggtccgatg cccaaccgca
     3901 cgtcggcctg atcgacatcg acggaacgat cacaccggtg atggccacct acgtcgggcg
     3961 ggggctcgat cgctcggccg accgcggcga tagcgccgtg atcctgcgga tggacacgcc
     4021 gggtgggctg tcgtcggcga tggacgatat cgtcagcgac atcctggcct cgccaatccc
     4081 cgttattgtc tatgtcgcgc cggagggcgc gcgggcggcc tcggccggcg tttacatcac
     4141 gtattccgca cacgtcgccg cgatggcgcc ggcgaccaac atcggctcgg cgacgccggt
     4201 caatctcggc gacgagtcga atgacgacaa tcagtcgacg atggatcgca aggtcatcaa
     4261 cgacgccgtc gccaagatcc gagggctggc ggagcggcgc gggcgcaacg ccgactgggc
     4321 cgaggcggct gtccgtgacg ccgacaacat caccgccagc gtggctgtcg aaaagaatgt
     4381 cgtcgatttt attgccggag accttgactc ggtcctcgcc caggcggacg gccggtccgt
     4441 cgtcgtccgt gggcagccgg tcacggtcga gacggccggt gcatcggtgt ccgagatcga
     4501 catgtcgttc ttcgagaagt tgctgcaggt cgtcaccgac ccgaacatcg ccttcgtgct
     4561 cgtcagcctc ggcacgctgg cgatcgtctt cgagatcgcc aatccgggcg ggatcggtcc
     4621 cggcgccgtc ggcggtctca tgctggtgac gggcttctac gcgctcggga cgctggatac
     4681 caactgggcc ggcctggcgc tgatggggct ggcgttcatg ctgttcgtga tcgatgtctt
     4741 tgtcccaact cacggcatcc tgacgatcac cggcatcatc tcgttcctgt tcggcgggct
     4801 gctgctgtcg aacacgcgca acgacgacgt gcttgagatc tcgcgggtgg tgatcttcac
     4861 catcacggcc ttgatgggtg tcttcttcct cttcctggcc ggtagtgcct ggcgctcgca
     4921 cggccgaccg ccgtcgaccg gcgagagtac gctgatcggt gcgatcgcgg tcgtccggca
     4981 gccgctggaa ccggatggga tggtgttcat ccagggcgag ctttggcagg cgacatcgac
     5041 ggacggcccc gtcgaacggg gacacttcgt gcggattctt gggattgacg gcctgcgcct
     5101 gaccgtgaca ccggtgacgg tcgaggccgg agtatcgccg gaggcgctgg agagcgcccc
     5161 gggataatgc tgctgcgcgg cggtggcgtc gcgccggata cccgcgtgcg ggtcatgacg
     5221 gaggactctg ttggataacg gcgtgttgat cgccctcggt gcgatcgtgc tattcatcct
     5281 gatcgtgctc tcagcatcga tcaagatcgt gcaggaatac gagcggggcg tcatcttccg
     5341 gctcggccgg ctgatgggcg cgcgtggccc gggcctgatc ctgctcatcc ccttcatcga
     5401 gcggatgatg aaggtcgatt tgcgaacgct gacgatggac gttccccgcc aggaggtcat
     5461 cacccgtgac aacgtgacgg tgaacgtcaa cgccgtcgtg tatttccgcg tcgtcgatcc
     5521 gaatgcggcc accgtcaata tcgccgacca cgttcgcgcc acgtcgcaga tcgcgcagac
     5581 gacgcttcgc tcggtgctcg ggcaggtcga attggaccag ctcctctccg agcgtgagaa
     5641 catcaaccag cgcttgcaag ccatcatcga cgagcaaacc gaaccgtggg gtgtcaaggt
     5701 cagcgtcgtc gaggtgaagg acgtcgagct gccgcagtcg atgcagcggg cgatggcgcg
     5761 ccaggctgag gccgagcgcg agaagcgcgc caaggtcatt catgccgaag gtgagttcca
     5821 ggcgtcacag cagctgcagg aagcagccgc gatcattgcc gaagagccaa tcgccatcca
     5881 gctgcgctac ctccagacgc tgaccgagat ctcggccgag aagaactcga ctatcatttt
     5941 cccgttgccg atggacctga tcgccgccgt cgttccaggc ttccagacat cgagtcaggc
     6001 atcggcgcat gagacgacaa ccacgccgcc ggcagaaccg actccaccac agcacgagga
     6061 gccggcatag tggggcggct acctgacgcg gttcgcgact ggcaggcgca actcacaccg
     6121 gtcaccgtcg acggcgtccg gagcttcgtg ctcgataccg gcatgggcga tccggtcgta
     6181 ttcctgcacg gcattccgac ccaggcgtat ctctggcgtg atgtcgcaca ggtcgtcagc
     6241 catcgctggc gggtgatcgc gccggatctg ctcggcttcg ggttctccga ccggcccgag
     6301 gatgcagact actcaccggg cgggcaggcg agcttcattc agcgtgtgct gagcgaactt
     6361 ggcgtcgagc gctacgcgct tgtcgtccac gattacggcg cgctggtcgg cgccgagcta
     6421 ctgactcgcg acccggacag cgtcaccaat atggtcgtcc tcaacacgag cttctgggag
     6481 gacgactgga gcggctcgca gctcaacccg ttcagcctgc tcagtctgcc cggtgttggc
     6541 gaggtcgctt ttcgggttgc gcgcaaattc atgctgcgcg aggccttcgg tctctacgtc
     6601 agcgagcggc aacgactgac ctctgacgta atggatgtct actggcatcc gttcactgat
     6661 ggcttcgctg atgtcctgct acggctgttc cgcgaccggg ccggccggat ggacgagcag
     6721 gccttctgcc gctggcgcga tgcgatcgag gcgttcgatc gccccagcct ggtcatctgg
     6781 ggcgaccgcg acccgacctt ccgcatctgg accggccgcc ggattgccga aatcctcccg
     6841 gccggcaacc tggagctgtt cgagcacagc aaccacttcg tccccgagga ccgccccgaa
     6901 gcgcttggcc gcctgatcaa ggtcctgctg agcgggcgtt acaaggggta acgcccctag
     6961 agggtgttat gaacttcggg taattctcga tacccttgga ggatggagac tacagcaacc
     7021 cgaaaacact atccgagcga catcagtgac gaagagtggg acctggtcgc cccctatctg
     7081 acgttgatga cggaggat
//
LOCUS       DASUGJ010000779         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_406011, whole genome shotgun sequence.
ACCESSION   DASUGJ010000779 DASUGJ010000000
VERSION     DASUGJ010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406011"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFV93_17605"
     CDS             <1..387
                     /locus_tag="VFV93_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167227.1"
                     /translation="DDLDGSLAACTEARDIMARSGDQRGVALADISHGWLELRRGDID
                     SAEHQFRAALATLQQIEAHVESAEAIEGLAAVADSRGERSAAIRQFHEAQALREATGI
                     HPDFMSRIGHQADRRALRARLSTTNP"
     gene            477..1859
                     /locus_tag="VFV93_17610"
     CDS             477..1859
                     /locus_tag="VFV93_17610"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5167228.1"
                     /translation="MLTMTTVDDERLDHALALNAKLLCSGVFVVGREPEEFIANDLQE
                     TNWFDWDAVDLTIDHENPSVTLAARGISRTALYNGRQGCTIIPRGEGGLHFTPLAFEP
                     NLPDPATTAWPLGNPEPNGPPPGVDHAALEAAFDRALDDAALPVPQMTRALVVVHGGR
                     VVGERYADGMGPDTRHVSWSSGKSITAALVGILAGEGVFNVDDPAPVAEWQAPDDPRR
                     AIKIIDLLRMSSGLKFVRHLQESPLYLSAANDHNMVYHGAIDVFRHSIDRPLEYPPNT
                     IWRYHNCDTLTLGALVRRTVEARGEDYLSYPQRALFDKIGIRNHILEVDPWGNFVMTG
                     FDYGTGRDWARFGLLHLWDGVWQPTGERILPEGWVDVVRTPAPADDTRSYGGQFWLNV
                     GGRHPSIPRDAFWAAGAWGQLTMVIPSRDTVIVRLGHTRDSESLDAYIDETFGSILAA
                     LPGLGMGDEA"
     gene            1924..>2294
                     /locus_tag="VFV93_17615"
     CDS             1924..>2294
                     /locus_tag="VFV93_17615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5167229.1"
                     /translation="MTVAESAPATTASVIRNRPLITLALGHFTIDLYAGLLPVLFPLL
                     EREYDLDLATVGLIALAYSGMGSISQPLFGWLADRHGTRLIGAALVWTAVTFSGIAFA
                     PSFPLILVLAGLAGLGSGAFHP"
BASE COUNT          374 a          829 c          732 g          359 t
ORIGIN      
        1 gacgacctgg atggttcact ggcggcctgc accgaggccc gggacatcat ggcgcgatcc
       61 ggtgaccagc gcggcgtcgc cctggccgac atcagccacg gctggctgga actgcgccgc
      121 ggcgacatcg acagtgcaga gcaccagttc cgcgctgcgc tggcgacgct gcaacagatc
      181 gaggcgcacg tcgaatctgc cgaagcgatc gagggcctgg ccgccgtcgc cgacagccgc
      241 ggggaacgtt ccgcagccat ccgccagttc cacgaggccc aggcgcttcg cgaggccacc
      301 ggcatccacc ccgacttcat gtcccgcatc ggccaccagg ccgaccgccg cgccctgcga
      361 gcacggttat caacaaccaa tccgtagggg cgcgcccacg gattgctgga ttgccatcat
      421 gcgccactgg cgcgacaatc gccccggctg atggtgttga ggggaaggcg gcggccatgc
      481 tgacgatgac gacggttgac gacgaacggc tggatcacgc gctggcgctg aacgcgaagc
      541 tgctctgttc cggcgtcttc gtcgtcggcc gagagccgga agagttcatc gccaacgacc
      601 tgcaggagac caactggttc gactgggacg ccgttgacct gacgatcgac cacgagaacc
      661 cgagcgtcac gctcgcggcg cgcggcatca gccggaccgc cctctacaac ggccgccagg
      721 gctgcacgat catcccgcgc ggcgagggcg gcctgcactt caccccgctc gccttcgagc
      781 cgaacctgcc cgatccggcc acgacagcct ggccgctggg caatccggag ccgaacggcc
      841 cgccacctgg agtcgaccat gctgccctcg aagccgcctt cgaccgcgcc ctcgacgacg
      901 cggccttacc cgtcccgcag atgacccgcg cgctggtcgt cgtccacggc gggcgagttg
      961 tcggcgaacg ctacgccgat gggatgggac cggacacccg ccacgtcagc tggtcgagcg
     1021 gcaagagcat cacggcggcg ctggtcggca tcctggctgg cgaaggcgtc ttcaacgtcg
     1081 atgacccggc gccggtcgcc gagtggcagg cgcccgacga cccgcgccgc gcgatcaaga
     1141 tcatcgacct gctgcggatg agcagcggcc tcaagttcgt ccgccatctg caggagagcc
     1201 cgctctatct ctcagccgcc aacgatcaca acatggtcta ccacggcgca atcgatgtct
     1261 tccgccactc gatcgaccgc ccactggagt acccgcccaa cacgatctgg cgctaccaca
     1321 actgcgacac gctgacactc ggtgcgctcg tccgccgaac ggtcgaggcg cgtggtgagg
     1381 actacctcag ctacccgcag cgggcgctgt tcgacaagat cggcatccgc aaccacatcc
     1441 tcgaagtcga tccctggggc aacttcgtga tgaccggctt cgactacggt accggccgcg
     1501 actgggcacg cttcggcctg ctccacctct gggacggcgt ctggcagccg accggcgagc
     1561 gcatcctgcc ggagggctgg gtcgacgtcg ttcgtacccc cgccccggcc gacgacacca
     1621 ggagctacgg tggtcagttc tggctcaatg tcggtggccg gcaccccagc atcccccgcg
     1681 acgccttctg ggccgccggc gcctggggcc agctgacgat ggtcatcccc tcccgcgaca
     1741 ccgtcatcgt ccgcctcggc cacacccgcg acagcgagag cctggacgcg tacatcgacg
     1801 agacgttcgg aagcattctg gcggcgttgc cgggactggg aatgggggat gaggcatgag
     1861 aaatgagtca agattgctcc tcatgcctca tcccccattc cccatgccca ggaacgcgcg
     1921 cccgtgaccg tcgctgagtc cgcaccggcc acaacggcga gcgtcattcg caaccgcccg
     1981 ctgattacgc ttgcgctcgg gcatttcacg atcgacctgt atgccgggtt gctgccggtt
     2041 ctgtttccgc tactggagcg cgagtacgac ctcgacctgg caacggtcgg gttgatcgcg
     2101 ctggcgtaca gcgggatggg gtcgatttcg cagccgctgt ttggctggct ggccgaccgg
     2161 cacggcacac ggctgatcgg cgccgcgttg gtctggacgg cggtaacgtt ctccggcatc
     2221 gccttcgcgc cgtcgttccc gctgattctg gtcctggccg ggctggccgg gctcggctcc
     2281 ggcgccttcc accc
//
LOCUS       DASUGJ010000780         3175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1516577, whole genome shotgun sequence.
ACCESSION   DASUGJ010000780 DASUGJ010000000
VERSION     DASUGJ010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516577"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..973
                     /locus_tag="VFV93_17620"
     CDS             110..973
                     /locus_tag="VFV93_17620"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5167230.1"
                     /translation="MSQDTGRGDRQPSNITEFRASRAGAARWPAGQRSALIVSIDVDG
                     TYGEANHHGVDDFYWRSQTEYDLQAGVWRLLDLLADRGVPATFCWVGRCVEDRPDAVL
                     RAHEDGHENAIHSWDHRYYTPMSFDEQLQDATRTIEALERVTGVRPLGHKTPAWRYND
                     DTSRVMQQLGLLWQMDKARDDLPWVERPDPDGSPLVQLPPSRFYDDYTYFVDWTVNPR
                     HAAEFWRDDLDVLRDEGKLLCLTLHPWVSGRPGPSRALANLLDYAIDAADIWIARADH
                     VAQWWRDQHGE"
     gene            complement(983..1624)
                     /locus_tag="VFV93_17625"
     CDS             complement(983..1624)
                     /locus_tag="VFV93_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167231.1"
                     /translation="MSLTFQTFEDASRAEALLASMGIRTESAPGWSTEHSHGDTASRS
                     VAGQFGIGCAVLLGLCMLFGVISALIGNAPGTSFSSVNAVVESESSSNGSNSDYRTAI
                     MSQSDELGRSMTRFTILSQDPQLFSDSWRIDVAAELAIWRSTYRDAIELSAPNEFRAF
                     HTTYLAALAEFDAAADKVERGLESMDPAFLDAAADDIRQGTALIDDALAVIPD"
     gene            complement(2164..>3175)
                     /locus_tag="VFV93_17630"
     CDS             complement(2164..>3175)
                     /locus_tag="VFV93_17630"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX5167232.1"
                     /translation="WSWVYTVWMTNWLDPDTRHVRIALIVLMLIGLLMSTSLTHAFDG
                     RALLFATAYVAGNVGRSLFILSAFRETPHQWRNFQRIISWQITGGVFWLAGGFADGST
                     RDLLWIIALTVEMAGPAAGFFVPRLGRSYTSEWTVAGDHFAERCELFLILSLGESILV
                     TGATVSELEFAFWTMVAFTVAFIGSVAFWWVYFDRGAEASSEIISHSDDSGRLARSAY
                     TYFHLPMVAGIILAAVGDELSIAHPRGETDTATAAVILGGPALFLAGHTLYKLAIFGY
                     FSRPRLAGIAALILLIPVASVTTPVALAALATTVIIGISIADVLHYRAMDVGEQPAPA
                     HD"
BASE COUNT          576 a          962 c         1096 g          541 t
ORIGIN      
        1 atatcgcaat atcgcccttt caaacatccg gcgcgtcgag aagggcgata ttgcgatatc
       61 gcccgtaccg gattgagcat tgattggtgt gtcgtgggaa ggtaggccga tgagtcagga
      121 cacgggccgg ggtgatcgcc agcccagcaa catcaccgag ttcagggcat cacgggcggg
      181 cgccgcgcgc tggccggccg ggcagcgttc ggcgttgatc gtcagcatcg acgtcgatgg
      241 gacgtacggc gaggccaacc atcatggggt agacgatttc tactggcgct cgcagaccga
      301 gtacgacctg caggcgggtg tctggcggct gctggatctg ctggccgacc gcggtgtacc
      361 ggcgacgttc tgctgggtcg ggcgctgcgt cgaggaccgg cccgatgcag tgctgcgcgc
      421 ccacgaggac gggcacgaga acgcgatcca ctcctgggac caccgctact acacgccgat
      481 gtcgttcgat gaacagctac aggacgcgac caggacgatc gaggcgctcg agcgggtgac
      541 gggcgtccgg ccgctgggcc acaagacgcc ggcctggcgc tacaacgacg acacgagccg
      601 ggtgatgcag caacttggcc tgctctggca gatggacaag gcgcgtgatg acctgccctg
      661 ggtcgagcgg ccagatccgg acggatcacc gctggtccag ctgccgccat cgcgcttcta
      721 cgacgactac acctacttcg tcgactggac cgtcaacccg cgccacgcgg ccgagttctg
      781 gcgcgacgat ctggatgtcc tgcgcgacga gggcaagctg ctctgcctga cgctgcaccc
      841 gtgggtcagc ggccggccgg ggccgtcccg cgcgctggcc aacctgctcg actacgcgat
      901 cgacgccgcc gacatctgga tcgcacgcgc cgaccacgtc gcccagtggt ggcgtgatca
      961 gcacggggag tgaccaggcg actcagtcgg gaatgacggc cagggcatcg tcgatcagcg
     1021 ccgttccctg tctgatgtca tctgcagcag cgtccaggaa ggctgggtcc atcgattcga
     1081 ggccgcgctc gaccttgtct gcggcggcat cgaactcggc cagggcggcg agatatgtcg
     1141 tgtggaaggc cctgaactca ttgggcgcgg agagttcgat cgcgtcgcgg tacgtcgacc
     1201 tccagatggc tagttcagcg gcaacatcga ttctccagct gtcgctgaag agttgtggat
     1261 cctgtgagag gatcgtgaag cgcgtcatgc tgcggccgag ttcgtcactc tgcgacatga
     1321 tcgctgtgcg atagtccgag tttgagccgt tcgaagacga ctccgactct acgacagcgt
     1381 tgactgaaga gaacgacgtg ccgggggcgt tgccgatgag cgcgctgata actccaaaca
     1441 gcatgcagag gccgaggagg acagcgcagc cgattccgaa ctggccggca actgaccgtg
     1501 acgctgtgtc gccgtggctg tgctccgttg accagccggg cgcagattcg gtgcggattc
     1561 ccatcgaagc gagcagcgct tctgcccggc ttgcatcttc aaacgtctga aacgtcagcg
     1621 acacgacttc gccacttgat cgtgtgacga gcaattctga gacgatgcgt ctcgtcgatg
     1681 tgatcagcat cgtctcgatg ttcaggagcg gtacgccgtt cgggttctcg atgcgaagcg
     1741 ttggcggagc gatttcaact cggttgccgc tcgccagaat gaacgaggtg gggctggttg
     1801 ccgggcctgg caggggagcc ggcggccgcg ccgtgtcgct gacctgcgca tcaggactga
     1861 gagaactccc acaggccggg cagtatctgg cgtccgcgct atgttgtgct ccgcaccagc
     1921 tgcagtatct aacctgccgc tgtggcgcgg taccttcgct tgtggtcaat gtgcgccccg
     1981 tccccgtgaa acgaccgcgt gacgcgtggt agatcaggga caatccgcgc tcattcagtg
     2041 acgacctcgt gagcgagcgt gcgttctgga tcgcttgcgg atggtcacgc caatattgtt
     2101 tcccgtgtgg gcggagttca ataggagcat cgtcccgtgt tggcgaaggc gtcgcgaatc
     2161 gcgtcagtcg tgcgcggggg ctggttgctc accaacgtcc atcgcgcggt agtgcaggac
     2221 gtcggcgatc gagatgccga taatgacggt cgtcgccagg gcggccagcg caactggcgt
     2281 ggtaacggac gcgaccggga tcagcaggat gagggcggcg atgccggcca gtcgtggacg
     2341 cgagaaatag ccgaagatcg ccagcttgta gagcgtgtgg ccggccagga agagggctgg
     2401 gccgccgagg atgacggcgg cggtagccgt gtcggtttcg ccgcgtgggt gggcgatgct
     2461 cagttcgtcg ccgacggcgg ccaggatgat gccggccacc atcggcaggt ggaagtaggt
     2521 ataggccgag cgcgccagcc ggccggagtc gtccgagtgg ctgatgatct cgctgctggc
     2581 ctccgcgccg cggtcgaagt agacccacca gaaggcgacg ctgccgatga aggcgaccgt
     2641 gaaggcgacc atcgtccaga aggcgaactc cagctcgctg acggtcgcgc cggtgaccag
     2701 gatcgattcg ccgagcgaga gaatcaggaa cagctcgcag cgttcggcga agtggtcgcc
     2761 ggcgacggtc cattcgctcg tgtacgacct gcccagacgg gggacgaaga agccggccgc
     2821 cggcccggcc atctcgaccg tcagtgcgat gatccagagc agatcgcgcg tcgatccatc
     2881 cgcgaacccg ccggccagcc agaagacgcc accggtgatc tgccaggaga tgatgcgctg
     2941 gaagttgcgc cactgatgcg gcgtctcgcg gaatgcagag aggatgaaca ggctccgccc
     3001 gacgtttccg gccacgtagg ccgtcgcgaa gagcagcgcc cggccgtcga aggcgtgcgt
     3061 cagcgacgtc gacatcagca ggccgatcag catcagcacg atcagcgcga tcctgacgtg
     3121 gcgcgtgtcc ggatcgagcc agttcgtcat ccagaccgtg tagacccagg accac
//
LOCUS       DASUGJ010000781         5064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1101216, whole genome shotgun sequence.
ACCESSION   DASUGJ010000781 DASUGJ010000000
VERSION     DASUGJ010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101216"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFV93_17635"
     CDS             complement(<1..202)
                     /locus_tag="VFV93_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167233.1"
                     /translation="MEQVTQESNDVTTLREQAMRGLLSRRAILKRGTALALSAPAIAA
                     LLAACGDDGDDDDGGNSSTNTSG"
     gene            403..978
                     /locus_tag="VFV93_17640"
     CDS             403..978
                     /locus_tag="VFV93_17640"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5167234.1"
                     /translation="MATVRQPATVLTAEDLLALPDDGKRYELVEGELQEIAPASGPHG
                     RDGGRIFGRLQQFTEQHDLGVMYLSDTGFQPGHDPVTVRCPDISFVRKSRLPETDEYG
                     VVPFAPDFVVEVVSPSNTVNEMTRKVQEYLEAGTEIVWVVEQMRRQVTVCTTGPFAQI
                     RRDGDTLDGGEVIPGFALPVTYIFDGTQATS"
     gene            complement(1002..1592)
                     /locus_tag="VFV93_17645"
     CDS             complement(1002..1592)
                     /locus_tag="VFV93_17645"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5167235.1"
                     /translation="MLRVECLTSIYPKIDREGVAVVTIMGAVAAELQAIGHRPNFFYL
                     QHAMGLASSMGLGIALGRPELQVVVFDGDGSVLMNLGGLTTLARYRPKNLVHVVFDNE
                     SLLSVGGFPTATSTGSDIEGMARAAGVLRTITVRECDDFIAAFDEALAADELTTIVAK
                     VEATGPKGFVTDLPLLENRFQFARHLRELRAKTASG"
     gene            complement(1663..2214)
                     /locus_tag="VFV93_17650"
     CDS             complement(1663..2214)
                     /locus_tag="VFV93_17650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5167236.1"
                     /translation="MGGHEPVGIFYGWWRGDELPDITPPAGLLIERLDDPPSSPTLDS
                     AEAATLRAEGHRLYVARIDTAITGHGWAATRTASIGEVGARMQLARNERYLWGFVTSP
                     EWRGQNIYPALIQAILRREADADRFWIGHDADNHPSARGVLKAGFTPVGAAYVGDDGV
                     LHYARYGEDERERAAQALLGMPP"
     gene            complement(2218..2805)
                     /locus_tag="VFV93_17655"
     CDS             complement(2218..2805)
                     /locus_tag="VFV93_17655"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5167237.1"
                     /translation="MEFQDVVRKRRLYREFDPERPVPRELLERIVKNGRRAPSAGHSQ
                     GWGFLVLETPEDRATFWDVTNRPGQEEATGSPSMHTATAIIVPFANKQAYLDRYAQPD
                     KGFPPNYEARWRIPHWYVDTAFASLTMLLSVVDAELGAIFFSVRDPAPLREAFGVPDG
                     FDPIGAIALGYPVPSGPPARHGRRPVDEVIHWGRW"
     gene            complement(2843..4180)
                     /locus_tag="VFV93_17660"
     CDS             complement(2843..4180)
                     /locus_tag="VFV93_17660"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5167238.1"
                     /translation="MSEAELRQVVETTMKELSVPGVAVGVIHDGKEYTAGFGVTNVNH
                     PQDVDADTLFQIGSISKTFLGTAVMRLVEQGKLTLDTPVSEYIPDFTMQDADAASRVT
                     VRQLLNHTAGWTGDYFDDTGMGDDAIDKMVRQMAEIPQLTPLGQLFSYNNAGFYVAGR
                     VIEIVTGQTFEAALKELVLDPLGMEMTFFFPDDVMVHRFAVGHSGQGDDIKVAQPWAL
                     ARCANPAGGLASTVNDMLKYARVQLGEGPQILSAESIATMRTPSVPIDSFGNHVGVTW
                     MLRNIGDVRIITHSGGTNGQISLFLTVPERNFAMVILTNNGSGGQLTQQVMKWALKHY
                     LDAEDPKPALQERTYEQLQEYAGAYQAAMSKLELSVEDGRLKVQVTPMGGFPKKDSPA
                     GPTPPPVRAGVIGKDQLITLDPPFADTRIDILRGPEGTIEWLRFGSRIARKQS"
     gene            4450..>5064
                     /locus_tag="VFV93_17665"
     CDS             4450..>5064
                     /locus_tag="VFV93_17665"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5167239.1"
                     /translation="MSTRTPDVADAVLGDLNGLLPGLEDLYKDIHAHPELSLQETRTA
                     GLAADRLRAAGFEVTTGVGKTGVVGLLRNGDGPVVMLRADMDALPIKEATGLPYASIV
                     TATDAEGATVPVAHSCGHDMHVTWLAGATTLLAQHREAWHGTLLAIFQPAEELGIGAQ
                     GMIDDGLFERFPRPAVVFGQHVMVGPAGVLGSRSGVITSAADSLQ"
BASE COUNT          822 a         1624 c         1698 g          920 t
ORIGIN      
        1 tgccggacgt gttggtcgag ctgttgccgc cgtcgtcatc gtcgccgtca tcgccacagg
       61 ctgccagcag cgcggcaatg gccggtgcgc tgagggccag ggcagtcccg cgcttgagga
      121 tggcgcggcg gctgagcagc ccgcgcatgg cctgctcgcg cagggtcgtc acgtcgttcg
      181 attcctgtgt aacctgctcc atgcagggga gctccttgcg tgtgtacccg tatcgaaccc
      241 atcgagaatc cgatatatcg gattcgtcaa aagcgaatgt tatcgttcac aatcaccaga
      301 tgtcaatgac gctccgcagg tccgatgagt ccgctgcgcc cgaacggcgg ctggtcgcca
      361 tcgaatgacg ctgtcatagc cagagcgtga ctcgagggat caatggcgac agtgcgacaa
      421 ccggccaccg tgctgacggc ggaggatctg ctggcgctgc cggacgacgg caagcggtat
      481 gagcttgttg aaggagaact gcaagagata gcccctgcga gtggacctca tggccgcgat
      541 ggcgggcgca ttttcgggcg gctccagcag ttcactgagc agcatgatct tggcgtgatg
      601 tacctgtcgg acaccggctt ccaaccaggt cacgacccgg tcactgtccg ctgcccggat
      661 atctcgttcg tccggaaatc gcgcctgccc gagactgacg agtacggcgt tgtgcccttt
      721 gcgcctgatt tcgtggtcga ggtcgtgtcg ccgtcgaaca ccgtcaacga gatgacacgt
      781 aaggtccagg aataccttga ggcagggacg gagattgtct gggttgtcga acagatgcgg
      841 cgacaagtga ccgtctgcac gaccggccca tttgcccaga ttcgacgcga tggcgatacc
      901 ctcgacggtg gtgaggtcat ccccggcttc gcgctacccg tgacctacat cttcgacggc
      961 acacaagcga cgagctgact ggcaggctgg atctcccttt gtcatcctga ggccgtcttc
     1021 gcgcgtagtt cgcgcaggtg gcgggcgaac tggaagcggt tctccagcag cggcaggtcg
     1081 gtgacgaagc ccttcgggcc ggtcgcttcg accttggcga cgatcgtcgt cagctcatcg
     1141 gccgccagtg cctcatcgaa ggcggcgatg aagtcgtcgc actcgcggac ggtaatcgtc
     1201 cgtaggacac ctgccgcccg cgccattccc tcaatgtcgg agccggtcga ggtggcggtc
     1261 gggaagccgc cgaccgagag caggctctcg ttgtcgaaga cgacgtggac gaggttcttc
     1321 ggccggtagc gggccagcgt tgtcagcccg ccgaggttca tcaggaccga gccgtcaccg
     1381 tcgaagacga cgacttgcag ttccggccgc ccgagcgcga ttcccagccc catcgacgag
     1441 gccagcccca tcgcgtgttg caggtagaag aagttgggtc ggtggccgat cgcctgcaac
     1501 tcggccgcga ccgcccccat gatcgtgacg acggccaccc cctcgcggtc gatcttcggg
     1561 tagatcgatg tcaggcactc gacacgcaac atggctgatc ccttcctgaa cctcgcgatg
     1621 gttgtatcag agtatcgcat ggtcccacga cttgaggcgt cgttacggcg gcattccgag
     1681 cagggcctgg gcggctcgct cgcgctcgtc ttcgccgtag cgcgcgtagt gcagcacccc
     1741 gtcgtcgcct acataggccg cgccgaccgg cgtgaagcct gccttgagga cgccgcgtgc
     1801 agatgggtga ttgtcggcgt cgtggccgat ccagaagcgg tcggcgtctg cttcccggcg
     1861 caggatggcc tggatcaacg ctggatagat gttctgccct cgccactccg gcgacgtcac
     1921 gaatccccac aggtagcgct cgttgcgagc cagctgcatc ctggcgccaa cctctccgat
     1981 cgaggctgtt ctcgtcgctg cccagccgtg gccggtgatc gcagtgtcga tccgggcgac
     2041 gtagagccgg tgaccttccg cgcggagggt tgctgcctcg gctgaatcga gcgttggact
     2101 agacggtggg tcatccaggc gctcgatcag caggccggct ggtggcgtga tgtccggtag
     2161 ctcgtcaccg cgccaccagc cgtagaagat gccgaccggc tcgtggccgc ccatacgcta
     2221 ccagcgaccc cagtggatca cctcgtcaac cggtcggcgg ccgtggcggg ccggtgggcc
     2281 gctggggacc gggtagccga gcgcgatcgc gccgatcggg tcgaagccgt ccgggacgcc
     2341 gaacgcctcg cgcagcggcg ccgggtcgcg gacgctgaag aagatcgccc caagctcggc
     2401 atcgacgacc gacagcagca ttgtcagcga ggcgaaggcg gtatcgacgt accaatgtgg
     2461 gatgcgccag cgcgcctcgt agttgggcgg gaagcctttg tcgggctggg cgtagcggtc
     2521 gagatacgcc tgcttgttgg cgaatggaac gatgatggcc gtcgccgtgt gcatcgatgg
     2581 cgagccggtc gcctcctcct ggccgggacg gttggtgaca tcccagaagg tcgcgcggtc
     2641 ctccggcgtt tcgagaacga ggaatcccca gccctggctg tggccggccg acggcgcccg
     2701 ccgtccgttc ttgacgatcc gctccagcag ctcgcgcggg acaggccgct cggggtcgaa
     2761 ctcgcggtac agccgccgct tgcggacgac atcctgaaac tccattggac gctccttcgg
     2821 tgataggcgg cctgttacct ggctacgatt gcttgcgcgc gatccggcta ccgaagcgca
     2881 gccactcgat cgtgccttcg gggccgcgca ggatgtcgat gcgggtatcg gcgaacggcg
     2941 ggtcgagggt gatgagctga tccttgccga tgacgccggc gcggaccggc ggcggcgtcg
     3001 ggccggccgg cgagtccttc ttcgggaagc cgcccatcgg ggtgacctgc accttcaggc
     3061 gaccgtcctc gaccgagagc tccagcttcg acatcgcggc ctggtaggcg ccggcgtact
     3121 cctgaagctg ctcgtacgtg cgctcctgga gcgccggctt cgggtcttcg gcgtcgaggt
     3181 agtgcttgag cgcccatttc atgacctgct gtgtcagttg gccgccggag ccgttgttgg
     3241 tcaggattac catcgcgaaa ttgcgctcgg ggacggtcag gaagagcgag atctggccgt
     3301 tcgttccgcc cgagtgggtg atgatgcgga catcgccgat gttgcgcagc atccaggtga
     3361 cgccgacatg gttgccgaag ctgtcgatcg gcacgctcgg cgttcgcatc gtcgcgatcg
     3421 actcggccga gaggatctgc ggcccctctc cgagctggac gcgcgcgtat ttcagcatgt
     3481 cgttgacggt cgaggcgagg ccgccggccg ggttggcgca gcgcgccagt gcccacggct
     3541 gcgcgacctt gatgtcgtca ccctgaccgc tgtgaccgac cgcgaagcgg tgcaccatca
     3601 catcgtcggg gaagaagaac gtcatctcca tcccgagcgg gtcgagcacc agctccttga
     3661 gcgccgcctc gaaggtctgg ccggtgacga tctcgatcac ccggccggcg acatagaagc
     3721 cggcgttgtt gtaggagaac agctgcccga gcggcgtcag ttgcggaatc tccgccatct
     3781 ggcgcaccat cttgtcgatg gcatcgtcgc ccatgccggt gtcgtcgaag tagtcgccgg
     3841 tccagccggc ggtgtggttg agcaactggc ggacggtgac gcggctggcg gcgtcggcgt
     3901 cctgcatcgt gaagtcgggg atgtattccg agacgggcgt atccagcgtc agcttgccct
     3961 gctcgaccag ccgcatgaca gccgtcccga ggaaggtctt cgagatcgag ccgatctgga
     4021 agagcgtgtc cgcatcgacg tcctgcgggt ggttcacgtt cgtgacgccg aacccggccg
     4081 tgtattcctt accatcgtgg atgacgccca ccgccacgcc ggggactgac aactccttca
     4141 tcgtcgtctc gacgacctgc cgcaattccg cctcgctcac gtcgctttcc ctctcctcgt
     4201 cctcgccgac cctgccggcc aacggcttga cagggtacta cgaggagtcg gaacaggaaa
     4261 cagaagtccg gggctcggca cgtatgggat ccggcgaaat acctctgttt ccctcatcga
     4321 tggtgcgatt tatgcaacca cgctaccggt acgtggcgct gtctgacgcg gggctctgag
     4381 actgacggac gccatgtctc acgatcagtg caggagacgt tcattcgcca acgagcgagg
     4441 aatactgcca tgagcacacg caccccggac gttgctgacg ccgttctcgg cgatctgaat
     4501 ggcctgctgc caggcctcga agacctctat aaggacattc acgcgcaccc cgaactatcg
     4561 ctccaggaaa cccgcacggc cgggctggcg gcggaccggc tgcgtgcggc cggcttcgag
     4621 gtgacgaccg gcgtcggcaa gaccggcgtc gtcgggctgt tgcgcaatgg ggatggcccg
     4681 gtcgtcatgc tgcgggcgga tatggacgcg ctcccgatca aggaggcgac cggcctgccc
     4741 tacgccagta tcgtcacggc gaccgacgct gagggggcga cggtgccggt cgcgcactcc
     4801 tgcggccacg atatgcatgt gacctggttg gccggcgcga cgacgctgct tgcccagcac
     4861 agggaggctt ggcacggcac gctgctggcg atcttccagc cggccgagga gcttgggatc
     4921 ggcgcgcagg ggatgatcga cgacggactc ttcgagcgct tccccagacc ggccgtcgtc
     4981 ttcgggcagc acgtcatggt cggccccgct ggcgtcctcg gctcgcgcag cggcgtcatc
     5041 acctcggccg ccgatagcct tcag
//
LOCUS       DASUGJ010000782         4440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1516638, whole genome shotgun sequence.
ACCESSION   DASUGJ010000782 DASUGJ010000000
VERSION     DASUGJ010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516638"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VFV93_17670"
     CDS             complement(<1..1289)
                     /locus_tag="VFV93_17670"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5167240.1"
                     /translation="MERDILDIIVKALFALIFVNALIELIRHRDRPRLESAALLGLLA
                     ISVLIEDIQQAADAEFDAGKIIALLLFSQPFLFLRLIGQFQPVPRRPIWISFACMVAS
                     WVIVLLSDETPPPIVTVVVVGLFMLVEAYAVAVLVQVTRRSSGIARLRLSAILAGSAL
                     VALYILLAGIVLAVPDTEDALRPSALPITIAVAVCLYIGFLPPRRLRRAVQMDELWRY
                     LSVMSGNAGHSQLATTLELASTSATRLLGGKGTVIALGTADGGRFQLRSTPEIEAQLA
                     TAGIETIERGPDSPAIERSLRERAPVACSTERPWDDGLTRLAAAFGGARSCLVAPLLF
                     QSAQAGVVIVFLDRLTLFLEDDLEMLRLLAGQAALAIDSSRRFDEARQESASRRALLD
                     LSQALADETDAEAVANRLATDLDTILPSASSGVLLPLP"
     gene            complement(1465..3069)
                     /locus_tag="VFV93_17675"
     CDS             complement(1465..3069)
                     /locus_tag="VFV93_17675"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX5167241.1"
                     /translation="MRSWHRRIVVAVVFALLAGLPVFSVSALAPQTSAFQRTWERTDH
                     DTNARPYRTWIWGPAAFSALMWEAYDEAPDGMRLVQYFDKSRMEDNSYREPNAPWDVS
                     NGLLVKELISGRMQVGDNHFEDRQPAGVNVAGDGDDTAGPTYSTFTDMLDLPPYDAGA
                     TITQRLARDGTISNDPSLAQYGATAAELVDLPGIRHRVASPFWTFMTSRGQIYENGSF
                     VDANLFENAYYATGLPITEAYWATVKVGNVQRDVLIQCFERRCLTYTPSNSAEWQVEM
                     GNVGQHYYVWRHGVPVPGEPPAITDTDYKPLDPAPLAGWPFDLDQQAWVLLQNDSPYT
                     MQVTLDGPISQTVTVDPKPGGIVYPNDGAFTGCDEDVPVGDVMLPPGNYRVKIDYLSG
                     NVIPGRDYWTLIPNALYSGCYYVVSDSGGSLSTATTGDVDIVDIFWDSEVPDEVSGEY
                     VVLQNIDDHAIQMQGWKIVDLQSNTYTFPDFVLQSGATVTVHNCTGPTDTSNLYTGAC
                     EATWNNGGDTATLLDAAGEVVDTFSY"
     gene            complement(3099..3884)
                     /locus_tag="VFV93_17680"
     CDS             complement(3099..3884)
                     /locus_tag="VFV93_17680"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX5167242.1"
                     /translation="MGLMEGKKCLIMGVANQRSIAWGIAQALHREGAQLSFTYAMERL
                     KDNVVKLISTLDGYEQMPLLECDVQDSGQVEALFKDLGSQWGSLDVFVHSVAFARQED
                     LSGNFMDLTWEGYALAHHVSAYSLIESSKHAQPLLEAAGGGSIMTLSYLAAERVVPKY
                     NVMATAKAALECNVRYLASELGPSNIRVNAISAGPLKTLAASAVKGISTARDIMEERA
                     PLRRNVTQDEVGNVGLFLASPLSSCVTGETIYADCGFNIMAIA"
     gene            4048..>4440
                     /locus_tag="VFV93_17685"
     CDS             4048..>4440
                     /locus_tag="VFV93_17685"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEX5167243.1"
                     /translation="MPIRRDGDQVRTIGTYENWLEQSIREAQRRGEFDNLAGQGKPQD
                     LTTDHHAGDRESGFRVLKNNGLAPAWIEADKAIRAEQAALAELAARTASFITEHLDDE
                     TANAPAPATPTFSTRLRRLWSGSQRPAQP"
BASE COUNT          824 a         1371 c         1484 g          761 t
ORIGIN      
        1 gggagtggca gcagaacgcc ggatgatgcg gacggaagga tcgtgtcgag gtcggtcgcg
       61 aggcggttgg cgacggcctc cgcgtcggtc tcatcggcca gcgcctgact cagatcgagc
      121 aacgcgcggc ggctggccga ctcctggcgg gcttcgtcaa agcgccggct gctgtcgatt
      181 gccagcgccg cctgaccggc cagtagcctc agcatctcaa ggtcatcttc gagaaagagc
      241 gtcagccggt ccaggaacac gatgacgacg cccgcctgcg cgctctgaaa cagtaagggc
      301 gcgaccagac acgagcgggc accgccgaac gcagcggcta accgggtcag accatcgtcc
      361 cacgggcgct cggtggagca ggcgaccggg gcccgctccc gcaacgaccg ttcgatcgcg
      421 ggcgaatccg ggccacgctc gatcgtctct atgccggccg ttgccaactg cgcctcgatt
      481 tccggcgtcg agcgcagctg gaaccggccg ccatcggccg ttccaagcgc gatcacggtt
      541 cccttgccac cgagcagccg cgtcgcggag gtcgacgcga gttcgagcgt ggtcgccagt
      601 tgggagtgcc cggcgttgcc gctcatgacg gacaggtagc gccagagctc atccatctgg
      661 acggcgcgcc gcaggcgacg gggcggcaga aagccgatgt agaggcagac agcgacggca
      721 atcgtgatcg gcaatgcgct gggccgcaac gcgtcttcgg tgtccggaac agccaggacg
      781 atcccggcca gcaggatata gagggcgacc agtgcggaac cagccaggat cgccgacagg
      841 cgcagccggg cgatgccgga cgagcgacgc gtcacctgca ccaggactgc aacggcgtag
      901 gcttcgacca gcatgaagag tccgacgacg acgacggtca ctatcggcgg aggcgtttcg
      961 tcgctcagca ggacgatgac ccaggatgcg accatgcagg cgaagctgat ccagatcggc
     1021 cgccggggga ccggctgaaa ctgaccgatc agacgcagga agagaaacgg ttggctgaag
     1081 aggagcagcg cgatgatctt gccggcatcg aactcggcat cggcggcttg ttggatgtct
     1141 tcgatcagga cactgatggc aagcagcccc agcagagcgg cggattcgag ccggggccgg
     1201 tcgcgatggc gaatgagctc gatgagcgca ttgacgaaga tcagcgcaaa cagcgcctta
     1261 acgatgatgt cgaggatgtc tcgttccaca ctcggcccat caatgcagcg gtcgttcgct
     1321 cggcagcggg ttccggagag cctaccacca ttatccgccg ccagccgggt ttcgtctgta
     1381 actgccgggc cggcatgaag cgtacgttcg tactaattct ccgttgtcat ccatcggctg
     1441 gcgcctcggg atgacaacgg agaattagta cgagaacgtg tccaccacct cgccggcagc
     1501 atcgagcagc gtcgctgtgt cgccgccgtt gttccaggtg gcttcgcaag cgccggtgta
     1561 gaggttgctc gtgtcggtcg ggccggtgca gttgtggaca gttacggttg caccggactg
     1621 tagaacgaag tccgggaatg tgtatgtgtt ggattgcagg tcgacgatct tccaaccctg
     1681 catctggatc gcgtgatcgt cgatattctg gaggacgaca tactcgcctg aaacttcgtc
     1741 aggtacctcg ctatcccaga agatgtcgac gatatccaca tcaccggtcg tcgccgtcga
     1801 taagctgccg cctgaatcag agacgacgta gtagcagccg ctgtagagcg cgttgggaat
     1861 gagtgtccag tagtctcggc cgggaatgac gttgccgctc aggtagtcga tcttgacccg
     1921 gtaattaccc ggcggcagca tgacatcgcc gaccggcacg tcctcgtcac agccagtgaa
     1981 ggcgccgtcg ttcgggtaga cgatgccgcc tggcttcgga tcgacggtca ccgtctggct
     2041 gatcgggccg tcgagggtga cctgcatcgt gtacggcgag tcgttctgca acagaaccca
     2101 cgcctgctga tcgagatcga acggccagcc cgccagtggc gccggatcga ggggcttgta
     2161 atcggtgtca gtgatggcgg gcggctcgcc gggaaccggg acaccgtgcc gccagacgta
     2221 gtaatgctgg ccgacgttgc ccatctcaac ctgccactcg gcgctgttgg agggcgtgta
     2281 tgtcaggcag cggcgctcga agcactggat caggacatcg cgctggacgt tgccgacctt
     2341 gacggtggcc cagtaggcct cggtgatcgg cagcccggtc gcgtagtagg cgttctcgaa
     2401 gaggttcgcg tcgacgaagc tgccattctc gtagatctgg ccgcgcgacg tcatgaaggt
     2461 ccagaacgga gacgcgaccc ggtggcgaat gccgggtaga tcgaccagtt ctgccgccgt
     2521 cgcgccgtac tgcgccagcg aagggtcgtt gctgatcgtt ccgtcgcggg ccagccgttg
     2581 ggtgatggtt gcgccggcgt cgtacggcgg gaggtcgagc atgtcggtga aggtgctgta
     2641 ggtcgggcca gccgtgtcgt cgccatcgcc agcgacgttg acgcctgccg gctggcgatc
     2701 ctcgaagtgg ttgtcgccaa cctgcatccg gccactgatc aactctttga cgagcaggcc
     2761 gttggagaca tcccacggag cgttcggctc gcggtagctg ttgtcttcca tccggctctt
     2821 gtcgaagtac tggaccaatc gcatgccatc aggcgcttcg tcgtatgcct cccacatcag
     2881 ggcgctgaac gctgccggcc cccagatcca ggtgcgatag gggcgggcgt tcgtgtcgtg
     2941 gtcggtccgt tcccaggtgc gctggaacgc ggaggtctgc ggcgcgagtg ccgagacgct
     3001 gaacaccggc aggccggcca gcaatgcaaa gacgaccgct acgactattc ggcgatgcca
     3061 ggaacgcatc tccaccccct ttgcgtcgat ccgttgcctc aggcgatcgc catgatgttg
     3121 aagccgcagt cggcgtagat cgtctcgccg gtgacgcagg acgagagcgg cgaggcgagg
     3181 aagaggccga cgttgccgac ttcgtcctgg gtgacgttgc ggcgcagcgg cgccctctcc
     3241 tccatgatgt cgcgggcggt gctgatgccc ttgacggcgc tcgcggccag cgtcttgagc
     3301 gggccggctg agatcgcgtt gacgcggatg ttgctcgggc cgagctcgga ggccaggtaa
     3361 cggacgttgc actccagcgc ggccttggcg gttgccatga cgttgtactt cggaacgact
     3421 cgctcggcgg ccagatagga gagggtcatg atgctgccgc caccggccgc ttcgagcaac
     3481 ggctgggcat gcttgctgga ttcgatcagc gagtaggccg agacgtggtg ggccagcgcg
     3541 taaccctccc aggtgaggtc catgaagttg ccggagaggt cttcctgacg ggcgaaggcg
     3601 accgagtgga cgaagacatc caggctgccc cactggctgc cgaggtcctt gaagagcgcc
     3661 tcgacctggc cggagtcctg aacgtcgcac tcaagcagcg gcatctgctc gtaaccgtcg
     3721 agcgtactta tcagcttgac gacgttgtcc ttcaaccgct ccatcgcgta ggtaaaggag
     3781 agctgggcgc cttcgcggtg gagcgcctgg gcgatgcccc acgcgatcga gcgctggttc
     3841 gcgactccca taatcaggca cttcttgcct tccatcagcc ccattgctgt gtcctgccct
     3901 tctttcgcgc gtatggcaat cggcaacgcc agtgtggggc aatgctaact gacccggcga
     3961 cgaagcgcac gtatcacaga aaccacgact cgatgacatt ggaagtaggg attgtataat
     4021 ccttcacaaa gtcgagagga ggcccgcatg ccaattcgac gtgacggtga ccaggttcgg
     4081 acgatcggca cgtacgagaa ctggctggag cagtcgatcc gcgaggcgca gcggcgcggc
     4141 gagttcgaca acctggccgg ccagggcaag ccgcaggacc tgacgaccga ccaccacgcc
     4201 ggcgaccgcg agagtggctt ccgcgtcctc aagaacaacg ggcttgcgcc ggcctggatc
     4261 gaggccgaca aagccatccg ggccgaacag gcggcactgg ccgaactggc agctcgaaca
     4321 gccagcttca tcaccgaaca cctcgacgac gaaacggcga acgcgcccgc gccagccacg
     4381 ccgacgttct cgacacgact gcgccgcctc tggtccggca gccagcgacc cgcccaacca
//
LOCUS       DASUGJ010000783         7863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_823629, whole genome shotgun sequence.
ACCESSION   DASUGJ010000783 DASUGJ010000000
VERSION     DASUGJ010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823629"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFV93_17690"
     CDS             complement(<1..678)
                     /locus_tag="VFV93_17690"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5167244.1"
                     /translation="MAERVIGVGVLGAGNIGGSVARAIVENADAIEQSSGLRLRLVGA
                     VNRSGTTKAGELGIDARLISTDAAALVDHPDVQVIVETMGGEQPAATLMARALANGKH
                     VVTANKEAVAKQMPALLAAARDGEAGFLCEASVGGGIPLIVSLRQIMATNRVERLRGI
                     VNGTTNFILSKMDAEGAGYAETLKEAQELGYAEPDPTADVEGFDASYKLAILASMMLG
                     RWVSPDEI"
     gene            873..1793
                     /locus_tag="VFV93_17695"
     CDS             873..1793
                     /locus_tag="VFV93_17695"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5167245.1"
                     /translation="MVSTPDIAPTVTSTNADFATFGAIHLDVTDGERSLGFWRDLVGL
                     DLLSVNGDVLHLGVAGRDLIVLHPGAGGPVVREAAGLYHLSFHLPSLEDFACAFARLE
                     AADYFQYPTDHGTHLANYVDDPDGIGLEFAFETTSEQRVAWNGLPGFEHNDDGDNWSA
                     RDPRYVAWLRFHASNGDTRIELPAGTVVGHIHLRAADLDAALAFYRDAIGFTVNSDGR
                     PKGFFDMSAGGTFPHRLACNNWESAGHPQRPAGTAGMRAFTLQFRSADALERAIAYLE
                     AAGNGIDRQPGDALVTDPSGTRLLLTTTRG"
     gene            2169..3230
                     /gene="asd"
                     /locus_tag="VFV93_17700"
     CDS             2169..3230
                     /gene="asd"
                     /locus_tag="VFV93_17700"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5167246.1"
                     /translation="MANGKIDVAVLGATGSVGQRYIELLDGHPQFQLAELFGSERSAG
                     RRYADIADWRTSADPPAAAANMVIKHHEEPVQSPIVFCSLPGGIAKEVESRLAAEGHA
                     VFSNARDNRLDPDVPLIIPEVNPEHARAIDTQRERRGWDNGFIVTNPNCSTIHLVLAL
                     KPIWDAFGIESCMVTTMQAVSGAGYPGVPSLDMIDNVVPYIGGEEEKMEIESKKLLGA
                     WSGGGFDYAGLAISAQCNRVPVRHGHTETVNLKLGKKAAPDEVIEVLRAFRGRPQELG
                     LHSAPESPIIVRDQPDRPQPIFDRDVASGMASVVGRVRECSIFDIKFVVLGHNTIRGA
                     AGASILNAELMLADGYIRA"
     gene            complement(3301..4215)
                     /locus_tag="VFV93_17705"
     CDS             complement(3301..4215)
                     /locus_tag="VFV93_17705"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5167247.1"
                     /translation="MKPTIVVPLDGSENAEQALPYARALAQRSEGSLLLVSVVDIPVE
                     FGAWSMASAGMYGIELDKWQQESHTYLQRIAGEQTGLTVTTMVRIGSAAAEILAATEG
                     AENPVIVTTSHGRTGLSRIFMGSVASKLVRESKCPVLVIRMREQPAAATPAFDRVLVP
                     LDGSPFSEEGLERAVDVFGGGLTLHLLRVVEVPTIPAAGMADGGLALNYGLIDEYMDA
                     TRDVAAEYLKEMTEKLTAEGHTVTADVRQGIVADEVLKAAAEHQADVIAMATHGRGGL
                     GRIFLGSVAERVLQEAERPLLLTKPQKR"
     gene            4426..6225
                     /locus_tag="VFV93_17710"
     CDS             4426..6225
                     /locus_tag="VFV93_17710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX5167248.1"
                     /translation="MSRRRFVAALASAGAGILAACSGPSEQRPASRYDRDVPTRAPNS
                     PDSFPTPTGQIGGTSSQIGQPIGPSSTPDSSPTSGNQPGTATTITGATVTTPTGFAVT
                     IDPDLPAHLQPQADRLRDQLAADPSLKAAGQLVISTIDNPADGTAVYPVDFVPVVSRR
                     LLVRNITIGELEGLWKGDITDWSALGSPVPHGVVRVTLESSAGPFAKDKAAGDFPTVD
                     ALADYFIQERGALALIPLDQLDFRFRALNIDDVNVLRPGDKAWPLRSFLTVTPQQTLD
                     TATANALTTALAPTLPAPVSMTWAGDIIIARQVQRRILETGDWAAPFRSIYPELTWAD
                     ITISNLETSLSDSFESITDPTTFTFKTDTPAIEGLQLAQIDVLSRANNHSFNYGDIGM
                     NDTTAVLDAAGIKHFGMGNNLDEARRAVVVEQNGVTFAFLGYNGISDDWDAAGPNYAG
                     TAPLVDWMVVEDIKREVAAGHIVIPYFHWGIEYQYDPTEEQRYFAQTAIDTGAAVVMG
                     SHPHWVQAVETYKGRPILYSLGNFVFDQEWSLETKQGMIAHLWMQGDKPLKIDIVPVL
                     IEDYHRPRVMTIEEQWLVLEHVWAASDWIIQNG"
     gene            6320..7519
                     /locus_tag="VFV93_17715"
     CDS             6320..7519
                     /locus_tag="VFV93_17715"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5167249.1"
                     /translation="MQTTYDIFSDLVRDDPYPLYRQMRDEAPVHFDGHAWNLTRHADV
                     LTAFTDPRFSSDRFGTPDWFEPDYALQIKPLYDITNLFILFMDPPDHTRLRGLVAQAF
                     SAKMVESVRPKIQAAVDELLDRVAPTGQMDITADLANPLPGIVIAEMLGVPKEDQPRF
                     KEWANAYARFLGTLGDNPELRDDANQAVIELSDYIREVAGQRRVEPRDDLITALVQAE
                     EQGERLTHDELVATCFLLLFAGNETTTNLIGNGVLTLLRHPVALRQLREQPAMIRTAI
                     EEFLRWESPVQFTDRAVIEDITIDGQRIEAGQYVRTILGAANRDPAQFDNPESLDLSR
                     RPNRHIAFAHGIHFCLGAPLARAEGQIAINTLVQRFPALAPASDRVEWQRNHVFRALK
                     SLPVTLQ"
BASE COUNT         1395 a         2828 c         2248 g         1392 t
ORIGIN      
        1 gatctcgtcc gggctgaccc agcggcccag catcatcgac gccaggatcg ccagcttgta
       61 gctggcgtcg aagccctcga cgtcggccgt cgggtcaggc tcggcgtaac cgagctcctg
      121 cgcctccttg agcgtctcgg cgtagccggc cccctcggcg tccatcttcg agaggatgaa
      181 gttcgtcgtc ccgttgacga tcccacgcag tcgctcgacc cggttagtcg ccatgatctg
      241 ccgcagcgag acgatcaacg gtatcccgcc accgacgctc gcctcgcaga ggaagccggc
      301 ctcgccatcg cgcgccgccg ccagcagggc cggcatctgc ttcgcgaccg cctccttgtt
      361 ggccgtcaca acatgcttcc cgttcgccag cgccctcgcc atcagcgtcg ccgccggttg
      421 ctcgccgccc atcgtctcga cgatcacctg cacatccgga tgatcgacca gcgctgccgc
      481 gtcggtcgag atcagcctgg cgtcgatgcc gagttcaccg gccttcgtcg tcccgctccg
      541 gttgacggcg ccaaccagcc gcagccgcaa tccgctcgac tgctcgatcg cgtccgcgtt
      601 ctcgacgatc gcccgcgcaa ccgaccctcc gatgttgccc gcgccgagaa ccccgacgcc
      661 aatgacacgc tccgccatgc tggttccctc ccaattcgca cacgacaacc gcggttcttc
      721 ctaccatcct ggcgtgaggg ttggcaactg gacggtcagt tcgttgggag atggggaata
      781 gccgttggac gtcgacgttt acgaaccgtg aacaggccgc atctgtctgt cgaatgttgc
      841 gatcgatgtt cgcatccgca cgggaggcac cgatggtgag cacgccggat atcgccccca
      901 ccgttacaag cacgaacgct gacttcgcaa ccttcggcgc gattcacctc gatgtcaccg
      961 acggggaacg ctcgctcggc ttctggcgcg atctggtcgg cctggacctg ctctcggtca
     1021 atggcgatgt cctccatctt ggcgtcgccg gccgggatct gatcgttctc catccgggcg
     1081 cgggcggtcc ggtcgtccgc gaggccgctg gcctctatca cctgtcgttc cacctcccgt
     1141 cgctggagga tttcgcctgc gcattcgccc gtcttgaggc agcggactac ttccagtatc
     1201 caactgacca cggcacccac ctcgccaact acgtcgacga tcccgacgga atcgggctcg
     1261 aattcgcctt cgagacgacc tcggagcaac gcgtcgcctg gaacgggctg cccggattcg
     1321 agcataacga cgacggcgac aactggagcg cgcgcgatcc gcgctatgtc gcgtggctca
     1381 ggtttcacgc ctcgaatgga gatacacgaa tcgagctacc ggctgggacg gtcgttggac
     1441 acatccactt gcgcgcggcc gacctcgacg cggcgctggc gttctaccgt gacgcgatcg
     1501 gcttcaccgt caactccgat ggccgcccga agggattctt tgacatgtcg gccggtggga
     1561 cgtttccgca ccggctcgcc tgcaacaact gggagagtgc cggccaccca cagcgacctg
     1621 ccggtacggc cgggatgcgc gcgttcacac tccagtttcg ctctgctgat gcgctggaaa
     1681 gagcgatcgc gtacctggag gcagccggga acgggatcga tcgccaaccc ggcgatgccc
     1741 tcgtaaccga tccgtccggt acacgtctgc tcctgacgac caccagaggg taggtaaacg
     1801 agggctgccg cgaccgcgtt gctgactgcc ggcaactgcg atcacggcgg tcttgacgac
     1861 cggccgtctg actgctatcg tcgcaattga gcaaagtcga ttgtgttgtc ggcagcgcgc
     1921 tcgttttgag ttcgccgcga caactggccc gggccatgtg gcccggacgg tgggatccag
     1981 aggtttcggc catgttcggg tctgtatcaa tctgggctga ggagactatg accgcgcgcg
     2041 ccggtacggc gacgcgtggg gctactgcct gacagtatcc cggcagcgcg aagacgtatc
     2101 ggcaggagta gcgactcctg cttttttgtt gcccgcattc tctgcggcgc tcacgaaagg
     2161 tgaatccaat ggcgaatggc aagatcgacg tcgccgtcct cggcgcgacc ggcagtgtcg
     2221 gccagcgcta catcgagctg ctcgacggtc atccgcagtt tcagctcgcg gaactgttcg
     2281 gcagcgagcg cagcgccggc aggcgctacg ccgacatcgc tgactggcgc accagcgccg
     2341 atccgcccgc tgcagctgcc aacatggtca tcaagcacca tgaggagccg gtccagtcgc
     2401 cgattgtctt ctgctcgctc cccggcggca tcgccaagga agtcgagtcg cgcctggccg
     2461 ccgagggtca tgccgtcttc tccaatgccc gcgacaaccg gctcgatccg gacgtaccgc
     2521 tcatcatccc cgaggtcaac cccgagcacg ccagggcgat cgacacccag cgcgagcgtc
     2581 gcggctggga caacggcttc atcgtcacca accccaactg ttcgacgatc cacctcgtcc
     2641 tggccctcaa gccgatctgg gacgccttcg gaattgagtc gtgcatggtc acgaccatgc
     2701 aggccgtctc cggcgccggc taccccggcg ttccgtcgct ggatatgatc gacaacgtcg
     2761 ttccctatat cggcggcgaa gaagagaaga tggagatcga atcgaagaag ctgctcggtg
     2821 cctggtctgg cggtggcttc gactatgccg gtctggccat ctcggcccag tgcaaccgcg
     2881 tcccggttcg ccacggccac accgagaccg tcaatctcaa gctgggcaag aaggcagcgc
     2941 cggacgaagt catcgaagtc ctgcgcgcct tccgcggccg ccctcaggag ttgggcctgc
     3001 actcggcgcc cgaatccccg atcatcgtcc gcgatcagcc cgaccgcccg cagccgatct
     3061 ttgaccgcga tgtcgccagt ggcatggctt ccgtcgtggg ccgagtccgc gaatgctcga
     3121 tcttcgacat caagttcgtc gtcctcggcc acaacaccat ccgcggcgcc gccggagcca
     3181 gcatcctgaa cgccgagctg atgcttgcgg atgggtacat ccgggcgtag gggaggagag
     3241 atggggcacg agggatgaga tcagggtcta cgtcgttcct catccctcat gccccatctc
     3301 tcatcgcttc tgaggcttcg tcaacagcaa cggccgctcc gcctcctgca atacccgttc
     3361 ggcaaccgag ccgaggaaga tccgccccag cccaccacgg ccgtgggttg ccatcgcgat
     3421 gacatctgcc tgatgttcgg ctgccgcctt caggacctcg tcagcgacga tcccctgccg
     3481 cacgtcggcc gtgacagtgt gaccctccgc cgtcagcttc tcggtcatct ccttgagata
     3541 ctcggcggcg acatcgcggg tggcgtccat gtactcgtcg atcaaaccgt agttcagcgc
     3601 cagcccgccg tcggccattc cggccgccgg aatcgtcggc acctcaacca cccgcagcag
     3661 gtgcagcgtc agcccgccgc cgaacacatc gacggcgcgc tccagccctt cctcgctgaa
     3721 cggcgagccg tccagcggca ccagcacgcg gtcgaacgcc ggagttgccg ctgccggctg
     3781 ctcacgcatg cgaataacca gaactgggca cttagactct cgaaccagct tggacgccac
     3841 gctgcccatg aagatccgcg ataagccggt ccggccgtgg ctcgtcgtca cgatcacggg
     3901 gttttcggcg ccttccgtcg cagccaggat ctcggccgcc gccgatccaa tccgaaccat
     3961 cgtcgtgacg gtgagccccg tctgttcgcc ggcgatgcgc tgcaggtagg tgtggctctc
     4021 ctgctgccac ttgtcgagct cgataccgta catgccggcg ctcgccatcg accacgcgcc
     4081 aaactcgacc gggatgtcga cgaccgacac cagcaacagc gatccctctg aacgctgcgc
     4141 cagagcccgt gcgtatggca acgcctgttc ggcattctcc gaaccgtcca acggcactac
     4201 aatcgtcggt ttcatcagcg ctccttctgc atcactccgg ctcctcagcc ggagtatagt
     4261 ctcccatggt cacaatcatg tcgtgtttcc gtcagatacc ttgcaaattc gtgtcgcgat
     4321 tcccccggcc tgtgtcagtc gcgatcgagg gcgtacactt gtgcgctgat tcaccggcag
     4381 accgttcgca tcgcacgggg gtagttgcgt atgcgccgca ttctgatgtc acgtcgccgt
     4441 ttcgtcgccg cgctcgccag cgccggagcc ggtattctag ccgcctgtag cggtccgagc
     4501 gaacagcgac ccgccagccg ctacgaccgc gacgttccaa cccgcgcgcc taactcccca
     4561 gacagctttc caaccccgac cggccagatc ggcggcacat ccagccagat tggtcaaccg
     4621 atcggcccat cctccacccc ggacagttca ccgaccagcg ggaaccaacc cggaacggcg
     4681 acgacaatca ctggcgcgac ggtcacgacg ccgaccggct tcgccgtcac gatcgatccc
     4741 gacctgccgg cgcacctgca accacaagcc gacaggctcc gcgatcagct cgccgctgat
     4801 cccagcctga aggccgccgg ccaactcgtc atcagcacga tcgacaatcc tgctgacggc
     4861 accgctgttt acccggttga cttcgtcccg gtcgtctcgc gccgcctgct ggttcgcaac
     4921 atcaccatcg gtgagctgga ggggctctgg aagggtgaca tcaccgactg gtctgcgctc
     4981 ggctcgcctg tcccacacgg tgtcgttcgt gtcacgctcg aaagctcggc cggcccgttc
     5041 gcgaaggaca aggccgccgg cgatttcccg accgtcgacg ccctggctga ctacttcatt
     5101 caggagcgcg gcgcgctggc gctgatcccg ctcgaccagc tcgatttccg cttccgcgcc
     5161 ctcaacatcg acgacgtcaa cgtcctccgc cccggcgaca aggcctggcc gctgcggagc
     5221 tttctcacgg tcacgcccca gcagacgctc gacaccgcaa cggcgaacgc cctgacgacg
     5281 gcgctcgcgc caaccctgcc tgcgcccgtc tcaatgacct gggccggtga catcatcatt
     5341 gcccgtcagg tccagcgccg cattcttgaa accggtgact gggccgcccc cttccgctcg
     5401 atctacccgg agctgacctg ggccgacatc acgatctcca acctcgaaac gtctctctca
     5461 gacagcttcg agtcgatcac tgacccgacg accttcacct tcaagaccga cacgccagcc
     5521 atcgaggggc tgcaactggc ccagatcgac gtcctctcac gcgccaacaa ccactcgttc
     5581 aactacggcg acatcgggat gaatgacacc accgccgtcc tcgacgctgc cggtatcaag
     5641 cacttcggga tgggcaacaa cctcgacgaa gcccgtcgcg ccgtcgtcgt cgagcagaac
     5701 ggcgtcacct tcgccttcct cggctacaac ggcatctccg acgactggga cgccgctggc
     5761 cccaactacg ccggtaccgc cccgctcgtc gactggatgg tcgtcgaaga catcaagcgc
     5821 gaggtcgccg ccggccacat cgtcatcccc tactttcact ggggcatcga gtaccagtac
     5881 gacccgaccg aggaacagcg ctacttcgcg cagaccgcga tcgacaccgg cgccgccgtc
     5941 gtcatgggct cacatccgca ctgggtccag gccgtcgaga cctacaaggg ccgtccgatc
     6001 ctctactcgc tcggcaactt cgtcttcgac caggagtggt cgctcgaaac caagcagggc
     6061 atgatcgccc acctctggat gcagggcgac aagcccctca agatcgatat cgtcccggtc
     6121 ctgatcgagg actaccaccg cccccgcgtc atgacgatcg aagagcaatg gctcgtcctc
     6181 gaacacgtct gggccgcctc cgactggatc atccagaacg gctgagtctg gtgaggttgc
     6241 gtcatcccgt cgaacgtgag acgctagagt cacgctgaaa accaacgctc catcctctca
     6301 tcgaccgagg agatctctga tgcagaccac ctacgacatc ttcagcgacc tcgttcgcga
     6361 tgacccgtat ccgctctacc gccagatgcg cgacgaagct ccggtccact tcgacggcca
     6421 cgcctggaat ctgacccgcc acgcggatgt tctgaccgcc ttcaccgacc cgcgcttctc
     6481 gtccgaccgc ttcggcacgc ctgactggtt cgaaccggac tatgcgctcc agatcaagcc
     6541 gctctatgac atcaccaacc tgttcatcct cttcatggac ccacccgacc acacccggct
     6601 gcgtggcctc gtcgcccagg ccttctcggc caagatggtc gagtccgttc gtccgaagat
     6661 tcaggctgct gtggatgagt tgctcgacag ggtggcaccg actggccaga tggatatcac
     6721 cgccgacctc gccaacccgc tgcccggcat cgtcatcgcc gagatgctgg gtgtgccgaa
     6781 ggaggaccag ccgcgcttca aggagtgggc caacgcctac gcccgcttcc tcggcacgct
     6841 cggggacaac ccggagctac gcgacgacgc caaccaggcc gtcatcgagc tgagcgacta
     6901 cattcgcgag gttgcagggc aacggcgtgt cgagccgcgc gacgacctca tcaccgccct
     6961 cgtccaggcc gaggagcagg gcgagcgcct tacccatgac gagctggtcg cgacctgctt
     7021 cctgctgctc ttcgccggca acgagacaac gaccaatctg atcggcaacg gcgtcctgac
     7081 tctcctgcgt catcccgtcg ctctccggca gctccgcgag caaccggcca tgatccggac
     7141 cgccatcgag gagttcctgc gctgggagtc gccggtccag ttcaccgatc gggccgtcat
     7201 cgaggacatc acgatcgacg gccagcggat cgaggccggt cagtacgtgc ggacgatcct
     7261 cggcgccgcc aaccgcgacc cggcccaatt cgacaacccc gaatcgctcg acctcagccg
     7321 ccggccgaac cgccacatcg cgttcgccca cggcatccac ttctgcctcg gcgccccact
     7381 cgcccgcgcc gagggccaga tcgcgatcaa caccctcgtc cagcgcttcc cggccctcgc
     7441 tccagccagc gaccgcgtcg aatggcaacg caaccatgtc ttccgcgccc tcaaatccct
     7501 gccggtgaca ctgcaatagt gaaccggcaa ttcgtagcgg ccgggtgccc gctgggcgtg
     7561 gtgactcgcc tcaatatcca atgaccaatg cgccgaatga tctattggta ttgggcgagt
     7621 caccgactcg ccgcaactgc acaaggggac gattgtcacg tcccagcatc ggcgtcccgt
     7681 tgtatactgc gcttcaggta tgcagatcag gatcgtcggg caagacccgg acgaccgcga
     7741 tgaatagagc gaattcgcga caactttccg agaaaggcga tctctggatg acgtatgtga
     7801 tcagcgaacc ctgtatcggc gtcaaggacg cctcatgtgt cgaagtctgc ccggtcgact
     7861 gca
//
LOCUS       DASUGJ010000784         6831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_963171, whole genome shotgun sequence.
ACCESSION   DASUGJ010000784 DASUGJ010000000
VERSION     DASUGJ010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963171"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1097)
                     /locus_tag="VFV93_17720"
     CDS             complement(15..1097)
                     /locus_tag="VFV93_17720"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5167250.1"
                     /translation="MLTIGVDAHKQVHAAVAVDALGREQAHWRGPNTPTGWQELAAWG
                     HALDAERQWGIEGAWHYGRGLAQTLVAAGDRVVEVNPRLTAGERRGGRERGKSDRLDA
                     RSVARVVNRDAVELPVVPAEDGTSVLALWSQERDQLQREATRLGNEAHQVLALLDPTY
                     QTTLGDLTTATAIAALVTCTVSDRDAVLAQARAAAVRRLGARLQLVHEQLAQVTASLE
                     AVGRQSLQPLDDLYGIGPLTASYLAGQLGPGKRFPTDACLANHSGAAPLEASSGEVVR
                     HRLNRGGNRQLNAILERIALTQGRGDPGARTYLARRRSEGRTDREARRALKRFICRAI
                     WRAWQDCPIPTLNDLAPFVIPADDSG"
     gene            1561..2001
                     /locus_tag="VFV93_17725"
     CDS             1561..2001
                     /locus_tag="VFV93_17725"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5167251.1"
                     /translation="MQSTDHLVVNEQLIERYDGISCLRNGAGQDHWNGLNYKLGINKK
                     TVGSQVLSMNVATVPPGGIAAAHIHVGFEVGLFIVQGRVLHKFGKGLRQSLENSAGDF
                     IFIEPGLPHEVYNLSESEPIVAVVARTSPNQWDEIIPYDPASDE"
     gene            complement(2029..2769)
                     /locus_tag="VFV93_17730"
     CDS             complement(2029..2769)
                     /locus_tag="VFV93_17730"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5167252.1"
                     /translation="MRESERPYDATYRSWYGKGAAQYDERFQGDFARLQAEFIVDLLQ
                     PQAGMRILDVGAGTGRASLPLAAAAPEAVVVAADLTPAMLQRAVEKRDAEGMAFPAPV
                     CANGRSLPFADGTFDAVVSIRVLHLFPTPHLGAFVDEMRRVLKPGGTLLVEFNSPFAG
                     FGWALGREVWRRVRFGRKPRHYLWPQHVDALFGGMQERTVYGFWVPGIGRLARANPRL
                     NGALRLARLRPPFSYLGDKILVRARKAE"
     gene            2862..4163
                     /locus_tag="VFV93_17735"
     CDS             2862..4163
                     /locus_tag="VFV93_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5167253.1"
                     /translation="MPEPHSRTLPEPAVAPAWEPTGDTPAIRITDVRAICTAPDGIRL
                     VVVKVETSEPGLTGLGCATFTQRPLAVVTAVEEYLRPFLIGRDPDDIEDIWQSAYVSS
                     YWRSGPVLLNALAGVDMALWDIKGKRAGMPIYQLFGGKCRSSATLYAHATGRDFREVE
                     EDARRWIEQGYRHIRCQVEIPRMATYGVHGPAGSEERPQPLGLDGGRWEPSAYCRTVP
                     KLFEHLRTTLGDEIELLHDIHERIPPIQAIGLAKALEPYNLFFLEDPFAPEDSGYFPL
                     LRQQTAIPIAMGELFVSQAEYVPLVRDRLIDFMRVHISAIGGLTPARKLAAYCELFGV
                     RTAWHGPGDVSPAGHAANLALDLSCWNFGIQEQHVFGEQTRAVFPGTPEIRDGALWPN
                     ERPGLGVEIDEALAVEFPFPDHPLNGGWPAVRRLDGTVVRP"
     gene            4188..4922
                     /locus_tag="VFV93_17740"
     CDS             4188..4922
                     /locus_tag="VFV93_17740"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5167254.1"
                     /translation="MRVAVISDMHGNAVAFDAVLADIRAQGADQIVCLGDTLQGGAQP
                     RQVLERLQEIDCPVVLGNADDFLRSADHGGEPSTELQLATRRWSLEQLGDDGLAAIGS
                     FLATVRIGLPGGRTLLCFHGSPTSFNDILLPETPEDEVQRLLGPSLPAIQCGGHTHLQ
                     QLRRVSDSFFFNPGSVGLAWNRNQPANPPLADPWAEYAILTADDTTVAVEFRRVPYDV
                     AAFIQIIRSSGIPFADELVERYSRQM"
     gene            5054..5662
                     /locus_tag="VFV93_17745"
     CDS             5054..5662
                     /locus_tag="VFV93_17745"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX5167255.1"
                     /translation="MSGIISRGFRGRRRNAETEGRLPPGQYLTTDFPVLLAGPTPRTS
                     LEQWDFRLTGEVETEQRWTWQEFQALPRETFTVDIHCVTRWSKLDTTWTGVSLDTLLD
                     AVEYDAEYITAFCDGGYTTNLPLEEVTGGRAWVVDTFDGQPLEPEHGGPARLLVPHLY
                     FWKSAKWVRGIQLTDHNEPGFWESLGYNNHGDPWREERYWED"
     gene            5760..6512
                     /locus_tag="VFV93_17750"
     CDS             5760..6512
                     /locus_tag="VFV93_17750"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX5167256.1"
                     /translation="MVATAARTRLGWHLATVVETRPESADAATVVFEIPGWPGHRAGQ
                     HVDVRLTAEDGYSATRSYSIASPPEEELVAITVESLPDGEVSPYLVEDVLAGDQLELR
                     GPVGGYFVWEAALGGPLQLIGGGSGLVPLMAMLRHHQATGSDELVRLLYSVRTPEDAL
                     FGAELDALAAASNNIEVVRTYTRRAPEGWTGHTRRIDADLLREVAYPASEQPLVYVCG
                     PTALVEGVAERLVEQGHDPARVRTERFGPTGR"
     gene            6525..6800
                     /locus_tag="VFV93_17755"
     CDS             6525..6800
                     /locus_tag="VFV93_17755"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEX5167257.1"
                     /translation="MSGDLTDTRLDGNAAAGLLGAIFPFEMTLAVAVCDGCGATAQVA
                     ALTVYAQAPGLIARCPGCQAVLLRVVEGPDRYWLDLQGLRSLELVGT"
BASE COUNT         1116 a         2352 c         2294 g         1069 t
ORIGIN      
        1 ctgacgctcc tatgtcaacc ggagtcgtct gccgggatga cgaagggtgc cagatcattg
       61 agggttggga tgggacaatc ctgccaggcg cgccagatcg cgcggcagat gaagcgtttc
      121 agtgcgcgtc gcgcctcgcg atcggtcctg ccctcgctac gccgccgggc gagataggtc
      181 cgtgcgcccg gatcgccgcg tccctgggtc agcgcgatcc gctccaggat ggcgttgagt
      241 tggcggttgc cgccgcggtt gagccgatgg cgcacgacct cgcccgagga ggcttccagg
      301 ggcgcggcac cgctatggtt ggccagacag gcatcggtcg gaaagcgctt gccgggaccg
      361 agctgaccgg ccagatagct ggccgtcagc ggcccgatcc cgtacagatc gtcgagtggt
      421 tgcagggact gtcgtccgac cgcctcaagc gaggccgtca cctgtgccag ttgctcgtgc
      481 accagctgga gccgcgcgcc cagccgccgg acggccgccg cgcgtgcctg ggccaggacc
      541 gcgtcccggt cgctgaccgt gcaggtcacc aacgccgcga tcgcggtcgc cgtcgtcagg
      601 tcgcccagcg tcgtctggta ggtcggatca agcagcgcca gcacctgatg ggcttcgttg
      661 cccagccggg tcgcctcacg ctggagctgg tcccgttcct ggctccacag cgccaggaca
      721 ctggtgccat cctcggccgg cacgaccggc agttcgaccg cgtcgcggtt gacgacacgg
      781 gccaccgagc gggcatccag ccggtcgctc ttgccccgtt cccgcccgcc ccgccgctcg
      841 ccagccgtca gccggggatt gacctcgacg acccgatcac cggccgcgac cagcgtttgg
      901 gccaggccgc ggccatagtg ccaggcacct tcgatccccc actgccgctc cgcatccagg
      961 gcgtgccccc acgcggcgag ctcttgccag ccggtgggcg tattcggccc gcgccagtgg
     1021 gcctgttcgc ggcccagcgc gtcaaccgcg acggcggcat ggacctgctt gtgcgcatca
     1081 acgccgatag tcagcatgct atcctcctgt ctgggagacc acttgccacg gcgccgggtt
     1141 ggacaggact ctctcgggtc acgaccggac gggctcctat caggtcacag cccggcgcac
     1201 cgcaggagcg ggcacacgac aagtcagatg cagggcacgc agccagtcat cctgtgggtc
     1261 agtgctgccc cgctccaccc tcattctcac agagagtcat cctgagccgc cgcgaaggat
     1321 cgcctggcca tggcgacgcg actggtgccc cagaatgtgc acgctcggct gccgaaccac
     1381 ccgcaagcgt ccctgacgcc aggcgatcct tcgctgcggc tcaggatgac agaggtgagc
     1441 ggtgctggtc ctcgtgcacg ccgtccggct ggtcgcacgt cccacgtccc gcgtcccgaa
     1501 tcccgagtac aatgaccggc atccggtcag gacgcgatca gagcacgagg gagtagcggt
     1561 atgcaatcga ccgaccatct cgtcgtcaac gagcagctta tcgagcgata cgacggcatc
     1621 tcctgcctgc ggaacggcgc ggggcaggac cactggaacg gcctcaacta caagctcggc
     1681 atcaacaaga agacggtcgg ctcgcaggtc ctctcgatga acgtcgcgac cgttccgccc
     1741 ggcgggatcg ccgcagccca catccatgtc ggcttcgagg tgggactctt catcgttcag
     1801 gggcgggtgc tgcacaagtt cggtaaggga ctgcgccaga gcctcgagaa cagcgccggc
     1861 gacttcatct tcatcgagcc gggcctgccg cacgaggtct acaacctcag cgagagcgaa
     1921 ccgatcgtcg ccgtcgtcgc ccgcaccagc ccgaaccagt gggacgagat catcccctac
     1981 gacccggcca gcgacgagta agaccgctgg gatggtccag ccgcagcgct attcggcctt
     2041 cctggcgcgg acgaggatct tgtcgccgag gtagctgaat ggtggccgga ggcgggcaag
     2101 ccggagggcg ccgttgaggc gcgggttggc gcgggcgagg cggccgatgc cggggaccca
     2161 gaagccgtag acggtgcgct cctgcatgcc gccgaagagg gcgtcgacgt gttgtggcca
     2221 gaggtagtgg cgtggcttgc ggccgaagcg aacccgccgc cagacctcgc ggccgagcgc
     2281 ccagccgaag ccggcgaagg gcgagttgaa ctccaccagc agcgttccac cgggcttgag
     2341 gacgcgccgc atctcatcga cgaacgcgcc gaggtgcggg gtcgggaaca gatgcagcac
     2401 ccggattgag acgacggcgt cgaacgttcc atcagcgaac ggcaggctgc gtccgttggc
     2461 gcagaccgga gccggaaagg ccatcccctc ggcatcgcgc ttctcgacgg cacgctggag
     2521 catcgccggg gtcagatcgg cggccacgac gaccgcctcc ggtgcggcgg cagccagcgg
     2581 cagtgacgct ctgcccgtcc cggcgccgac atcgaggatg cgcatgccgg cctgcggctg
     2641 aagcaggtcg acgatgaact ccgcctgcaa cctggcgaag tcgccctgaa agcgctcgtc
     2701 gtactgcgcg gcgcccttgc cgtaccaact ccgataggtc gcgtcatagg gtcgttcaga
     2761 ttcccgcacc gaaccggctc ctcaactgcg ccagactccg acaccggtgg tagcatggcg
     2821 ctcgatctca acagtcgcat cgtcgcgtcg agaggatacc aatgcctgag cctcactcgc
     2881 gaacgctgcc ggagccggcc gtcgcgccgg cctgggagcc aaccggcgac acgccggcga
     2941 tccgcatcac cgacgtccgc gcgatctgca cggcgccgga cgggatcagg ctggtcgtcg
     3001 tcaaggtcga gacgagcgag ccgggactga ccggcctcgg ttgcgcgacc ttcacccagc
     3061 gcccgctggc ggtcgtgacg gcggtcgagg agtacctcag gccgttcctg atcgggcgcg
     3121 atccggacga catcgaggac atctggcagt cggcttacgt ctcctcatac tggcgtagcg
     3181 ggccagttct cctcaacgcg ctggccggtg tcgacatggc gctctgggat atcaagggca
     3241 agcgggccgg gatgccgatc taccagttgt tcggcggcaa gtgccgctcg tcggccacgc
     3301 tctacgccca cgcgaccgga cgcgacttcc gcgaggttga ggaggacgcg cgccgctgga
     3361 tcgagcaggg ctaccggcac atccgctgtc aggtcgagat tccccggatg gcgacctacg
     3421 gcgtccacgg cccggctggc agcgaggagc ggccccagcc actcgggctg gacggcggcc
     3481 gctgggagcc atccgcctac tgccggacgg tgccaaagct gttcgagcac ctgcggacaa
     3541 cgctgggcga cgagatcgaa ctgctgcacg acatccacga gcgcattccg ccgattcagg
     3601 cgatcggact ggcgaaggcg ctggagccgt acaacctgtt cttcctcgaa gacccgttcg
     3661 cgccggagga cagcggttac ttccctctcc tgcgccagca gacggcgatt ccaatcgcga
     3721 tgggcgagct gttcgtcagc caggcggagt acgtcccgct cgtccgcgat cggctgatcg
     3781 acttcatgcg cgtccacatc tctgccatcg gcgggttgac accagcccgc aagctcgcgg
     3841 cctactgcga gctgtttggc gtccggaccg cctggcacgg ccccggcgat gtctcgccgg
     3901 ccggtcacgc cgccaatctg gcgctcgacc tcagctgctg gaacttcggc atccaggagc
     3961 agcacgtctt cggcgagcag acgcgggccg tcttccctgg tacgccggag atccgcgacg
     4021 gcgcgctctg gcccaacgag cggcccggcc tgggcgtcga gatcgacgaa gcactggcgg
     4081 tcgagttccc attccccgat catccgctca acggcggctg gccggccgtc cgccggctgg
     4141 atggcaccgt cgtcaggccg tagtctgttg ctggagagga acacgcaatg cgcgtcgcgg
     4201 tgatttcgga catgcacggg aacgcggtcg cgttcgacgc ggtcctggcg gacatccgcg
     4261 ctcagggggc cgaccagatc gtctgcctcg gtgacacgct ccagggtggg gcgcagccgc
     4321 gccaggtgct ggagcgcctc caggagatcg actgcccggt cgtgctcggc aatgctgacg
     4381 acttcctgcg cagcgcagac cacggcggcg aaccatcgac ggagctgcaa ctcgcgaccc
     4441 gccggtggag cctggagcag cttggcgacg acggcctcgc ggcgattgga tcgtttctcg
     4501 caaccgtcag gatcggtctg ccgggcgggc ggacgctgct gtgcttccac ggctcgccaa
     4561 cctccttcaa cgacatcctg ctgcccgaga cgcccgaaga cgaggtccag cggctgctgg
     4621 ggccatccct gccggcgatc cagtgtggcg gccacaccca cctgcagcag ttgcgccgag
     4681 ttagcgactc gttcttcttc aatccaggca gcgtcggcct ggcctggaac cggaaccagc
     4741 cggccaaccc gccactggcc gacccatggg ccgagtacgc gatcctgacc gccgacgaca
     4801 cgaccgtcgc agttgagttc cgccgcgttc cctacgacgt cgccgcgttc atccagatca
     4861 tcagatcgag cggcatcccg ttcgcggacg agttggtgga gcgctatagc cggcaaatgt
     4921 aactgctcgt ccggaacacc gccgccgggc gaatgtcacg tcgggaacga atgcgggacg
     4981 atactggtta gaaacgtcac gacacgatcg cgcgtgtcgc gccgcccgac atgccggaaa
     5041 gcaggtgagc acgatgagtg gaatcatctc acgcggattt cgaggccgtc gccgcaacgc
     5101 cgagaccgag gggcggctgc caccaggcca gtatctgacg accgacttcc cagtcctctt
     5161 ggccggcccg acgccacgca cgtcgctcga gcagtgggac ttccgcctga ccggcgaggt
     5221 cgagaccgag caacgctgga cctggcagga gttccaggcc ctgccacgcg agacgtttac
     5281 cgtcgacatc cactgcgtca cgcgctggtc gaagctggac acgacctgga ccggcgtctc
     5341 gctcgacacc ctgctcgacg cggtcgagta cgacgccgag tacatcactg ccttctgcga
     5401 cggcggctac acaaccaacc tgcccctgga ggaggtcacc ggcggccgtg cctgggtcgt
     5461 cgacacgttc gacggtcagc cgctggagcc ggaacacggc ggacccgccc ggctgctggt
     5521 gccgcatctc tacttctgga agagcgccaa gtgggtgcgc ggcatccagt tgacagacca
     5581 caatgaaccg ggcttctggg aatcgctcgg ctacaacaac cacggcgacc cgtggcgcga
     5641 agagcggtat tgggaggact agcgcggttg tgtgatgcgt catgcgtgat gcgggccgag
     5701 cgtgagaaga tcccttccgc atcacccatc acctatcatg ccccagatcg gaggtcgata
     5761 tggtcgcaac ggcggcccgt acccggctcg gctggcacct ggcaacggtc gtagagacgc
     5821 ggcctgagtc ggctgatgca gcgacggtcg tctttgagat ccccggctgg cccgggcatc
     5881 gggcgggaca gcatgtcgac gtccggctga cggccgaaga tggctactcg gcgacgcgct
     5941 cctactcgat tgcctcgccg cccgaggagg aactggtcgc gatcaccgtc gagtccttgc
     6001 cggatggcga agtctctcca tacctggtcg aggatgtcct ggccggtgac cagcttgagc
     6061 tacgcggccc ggtcggtggc tacttcgtct gggaggcagc tttgggcgga ccgctgcagc
     6121 tgatcggcgg cgggtcgggg ctggtcccgc tgatggcgat gctgcggcat caccaggcga
     6181 ccggcagcga cgagctggtc cggttgctgt actcggttcg aacgccggag gacgcgctgt
     6241 tcggtgccga gcttgatgcg ctggctgcgg cgagcaacaa catcgaggtc gtccgcacct
     6301 acacccggcg agcgccggag ggctggaccg gccacacccg ccggatcgac gccgatctgc
     6361 tgcgtgaggt cgcttacccc gcgtcggagc agccgctggt ctacgtctgc ggcccgaccg
     6421 cgctggtcga gggtgtcgcg gaacggctcg tcgagcaggg acatgacccg gcccgcgtcc
     6481 gcacggaacg ctttggccca accggcagat agaggaggaa ggccatgtct ggcgatctga
     6541 ctgatacccg gctggacggc aacgccgccg ccgggctgct cggcgcgatc ttcccgttcg
     6601 agatgacgct ggcggtcgcc gtctgcgatg gctgcggcgc gaccgcccag gtcgcagccc
     6661 tcaccgtcta cgcgcaggcg ccgggcctga tcgcccgttg ccccggctgc caggccgtcc
     6721 tgctgcgcgt cgtcgagggg ccggaccgtt actggctcga cctgcaaggt ctgcgctcgc
     6781 tggaactggt tggcacctag ccgccctgca acgcacatgt gccaaccctg t
//
LOCUS       DASUGJ010000785         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2074426, whole genome shotgun sequence.
ACCESSION   DASUGJ010000785 DASUGJ010000000
VERSION     DASUGJ010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2074426"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFV93_17760"
     CDS             complement(<1..595)
                     /locus_tag="VFV93_17760"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX5167258.1"
                     /translation="MFYLDPLYFVFMLPAIGFVMFAQWRVTSTFKKFSNVPTNHRMTG
                     AQVAQEILRTNGIYDVPVEPVPGQLSDHYDPRTKTLRLSEPVYGSTSVAAVGVAAHEV
                     GHALQHAQAYAPLKIRSAIVPVANAGNLLGPIMVIAGVLIGLTGLAWLGVIFFAAGTL
                     FALVTLPVEFNASSRAMVQLTNIGAVDRTEYGQARKVL"
     gene            810..1853
                     /locus_tag="VFV93_17765"
     CDS             810..1853
                     /locus_tag="VFV93_17765"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5167259.1"
                     /translation="MPLETFDRQLDNAILTAITNAETDAAPSGHGDLPIYDVLRYHLG
                     FVNERFEPERVNAGKRVRPRLCALACEAAGGDPSRAVFAAAAIELLHNFTLIHDDIQD
                     QSVYRRHRRTVWDLWGSAQAINAGDAMFAIAHHALNHSTEAGVDSATVLELSNRLHDA
                     TLRIVEGQVLDLGFEQREDVSTANYLTMIGGKTGALTRFACWTGARIAAAKASQVEQL
                     AEFGNVLGVGFQLRDDILGVWGETGDTGKAEADDIRRRKKSLPILLLRERATGPELAT
                     LDGIYEHDDVTDDEIQSVLELMRFHEVEFDVQTQVRAWHDRARDLLSSAIAPSPAADE
                     LFALVESLERRAG"
     gene            complement(1850..>2528)
                     /locus_tag="VFV93_17770"
     CDS             complement(1850..>2528)
                     /locus_tag="VFV93_17770"
                     /inference="COORDINATES: protein motif:HMM:NF020318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HEX5167260.1"
                     /translation="EIPSLYAIAVQDHGYLPGSGGREFRYEFLQSLLEGGGTVERMVY
                     HDPPEYMIRMRSVIDSLPGAVVMDTGAAAVAGVLGDPVVSQAADGEGAILVNIGNMHT
                     FGVAMRGRRVFGLFEHHTGGITPDLLDHLVGSLQAGTLTHEEVTANGGHGAAFDPAYR
                     QAGRFPFVAITGPNRRVAAPLNYHAAVPHGDMMLAGPFGLVEGMLQRLSSAGYELTVT
                     TLVGATS"
BASE COUNT          462 a          841 c          821 g          404 t
ORIGIN      
        1 tgagcacctt gcgggcctgc ccgtactcgg tccggtcgac ggcgccgatg ttagtcaact
       61 gcaccatcgc tcgcgacgag gcgttgaact cgaccggcag cgtcaccagc gcgaacagcg
      121 taccggctgc gaagaagatc acgcccagcc aggcgagacc agtcaggccg atcaggacac
      181 cggcgatgac catgatcggg ccgagcaggt tgccggcgtt agcgaccggc acgatcgccg
      241 agcgaatctt gagcggcgca tacgcctgcg cgtgctggag tgcgtgaccc acctcgtggg
      301 ccgcgacgcc gacggccgcg actgatgtcg aaccgtagac aggctcagag agccgcagcg
      361 tcttcgtccg tgggtcgtag tggtcgctga gctggccggg gaccggctcg accggcacgt
      421 cgtagatgcc gtttgtgcgc aggatctcct gagcgacctg ggcgccggtc atccggtggt
      481 ttgtgggcac gttcgagaac ttcttgaacg tgctcgtcac ccgccactgg gcgaacatga
      541 cgaacccgat cgccggcagc atgaacacga agtagagcgg atcaaggtaa aacatggctt
      601 ctcactttcc gaatcgtcgc aatcacggcg attcacaccg ggtcacccag aaccatgctg
      661 tcacgtttcc agaatcgcga ggtccgggct ccggtgacgg cgggcgaaca tctgttcccg
      721 ccatgctata gtatgccgca gcgtgaaccg cgcttcaatc ggcgggcgga tgcctggctg
      781 accgcgaaag gaccaggcga acacgccaga tgccgctcga aaccttcgac cgccagctcg
      841 acaacgccat cctgactgcc atcaccaacg ccgagaccga cgccgctccc agcggccacg
      901 gtgacctgcc gatctacgac gttctgcgct atcacctcgg cttcgtcaac gaacgcttcg
      961 agcctgaacg ggtcaacgcc ggtaagcgcg tccggccgcg tctctgcgcg ctggcctgtg
     1021 aggcagccgg tggtgatccg tcgcgggcgg tcttcgcggc agcggccatc gagctgctgc
     1081 acaacttcac gctaattcac gacgacatcc aggatcagag cgtctatcgc cgccaccgcc
     1141 gaacggtctg ggatctgtgg ggatcggccc aggcgatcaa cgccggtgac gcgatgttcg
     1201 ccatcgccca ccacgcgctc aaccactcga ccgaagcggg agtcgattct gcgacggtgc
     1261 tggaactttc gaaccgtctc cacgacgcga ccctgcggat cgtcgaaggt caggtgctcg
     1321 acctgggctt cgagcagcgc gaggatgtgt cgaccgcgaa ctatctgacg atgatcggcg
     1381 gcaagaccgg cgcgctgacc cgcttcgcct gctggacggg cgcgcggatc gccgcagcga
     1441 aggcatcgca ggtcgagcag ctggccgagt tcggaaacgt gctgggcgtc ggattccaac
     1501 tgcgcgacga catcctcggc gtctggggcg agaccggcga caccggcaag gccgaggccg
     1561 atgacatccg ccggcgcaag aagtcgctgc cgatcctgct gctgcgcgag cgggcgaccg
     1621 gcccagaatt ggcgacgctg gatggcatct acgaacacga cgatgtcacc gacgatgaaa
     1681 tccagtccgt cctggagttg atgcgctttc acgaggtcga attcgatgtc cagacccaag
     1741 ttcgcgcctg gcacgaccgc gcccgcgacc tgctctccag cgccatcgcg ccatcgccgg
     1801 ccgcagacga actgttcgcg ctggtcgaat cgcttgaacg ccgggcaggc tagctggtcg
     1861 cgccgaccag cgtcgtgacc gtcagttcgt agccggctga cgacaggcgc tggagcattc
     1921 cctcgacaag gccgaatggg ccggccagca tcatgtcacc gtgtgggacg gccgcatggt
     1981 agttcaacgg cgcggcgaca cgccggtttg gcccggtgat cgccacgaac ggaaaccgcc
     2041 ccgcctgccg gtaggctggg tcgaacgccg cgccatgacc gccgttggcc gtgacctctt
     2101 cgtgcgtcaa cgtcccagcc tgcaagctcc ccacgagatg gtcgagcagg tccggggtga
     2161 tgccgccggt gtggtgctcg aagaggccga agacgcgccg gccgcgcatc gcgacgccga
     2221 aggtgtgcat gttgccgatg ttgacgagaa tggccccctc accgtccgct gcctggctga
     2281 cgaccggatc gccaaggacg ccggcaaccg ccgccgcgcc ggtgtccatc acgaccgcgc
     2341 cgggcaggct gtcgatgacg ctgcgcatgc ggatcatata ttcgggcggg tcgtgataga
     2401 ccatccgttc aacggtacca ccaccttcca gcagcgactg taggaactcg tagcggaact
     2461 cgcgcccgcc ggagccgggc aggtagccgt ggtcctgaac ggcgatcgcg tagagcgacg
     2521 gaatctcg
//
LOCUS       DASUGJ010000786         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_546878, whole genome shotgun sequence.
ACCESSION   DASUGJ010000786 DASUGJ010000000
VERSION     DASUGJ010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546878"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..920)
                     /locus_tag="VFV93_17775"
     CDS             complement(387..920)
                     /locus_tag="VFV93_17775"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEX5167261.1"
                     /translation="MQEEQSQPIEGDMNELNRRISVAWQALEAAIAGLDEQQLSDVRD
                     ANGWAIKDHLMNVVLWERSIAQLLLNQPRHETLGVSEDDYLNLGHDGINAIIFEQHRD
                     LSAGEVLARLLQQHEETLEILNRFTWDDLLRPYSHYLPDEPGKDNGDPILYWIMGNTA
                     GHYDEHRQWIEALRDGR"
     gene            complement(957..1493)
                     /locus_tag="VFV93_17780"
     CDS             complement(957..1493)
                     /locus_tag="VFV93_17780"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX5167262.1"
                     /translation="MNAYLETVDSPAGPLTFAVNEDGALLFLSFLEGHYPTTIEEDLI
                     NEGYTCLSDPERTAHVRQQLVEYASGERTTFDLPVVLQGTEWQKAVWRELMQVPFGET
                     RTYGELARSLGRPQASRAVGRANGTNRIPLVIPCHRIIGADGTLTGFGGGLHLKTALL
                     THEGVEIAKPAKQLALAT"
     gene            complement(1513..>2556)
                     /locus_tag="VFV93_17785"
     CDS             complement(1513..>2556)
                     /locus_tag="VFV93_17785"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5167263.1"
                     /translation="LRVAAARRMLLHGQRPVAEIAFEVGFESLSAFNENFRKYTAMSP
                     ARYRQLPDEAAFDLRLPDDYPLGRILAYLGRDKNSLTERVEGQTCLIAYRAGEQPALV
                     RIVFEDGLARCTVLLPEQPNPDLMEALHSRVLATLGLTVDPARFEALVTGSPELAPLI
                     DGQRGLRVPLLADPFDGLVWAIVGQQINLAFAYTLRHRLIEHVSQPLGEGLYAPPSVA
                     AVAAIEPEDLLPLSFSRAKASYLVGAARSVIDGRLPLDALAGSSATRIERTLLAVRGI
                     GPWSAHYLMMRHYGFLDCVPLGDTGLTSGLKQFFALDERPGKLETLALMDRFSPYRSL
                     ATFHLWQRHGAVA"
BASE COUNT          422 a          821 c          881 g          432 t
ORIGIN      
        1 tcacatcgac ggctcgcgtt tggtgatggg ggtgggtggg gagacggact ggtgagtcat
       61 gcggcgagag gaacgtttac ggaaggggcg ccaagcgtgg tgcgtgtcgt ggcgagggtg
      121 acggggttga gtggttgcag cgatgaacga tgtcgcagcg tccaagtagg ggagggtcgt
      181 tgcacccaat tcgaaactta agacgtcgaa agacccgccc ggctccccac cccacaccgt
      241 caccctcgcc gcgacacgca ccacgccacg ctcccaccca accccgtcac cctcgcgtca
      301 actgctggag aatcgaatga gtatggcgcg ccacagttcg ctgcactgat acgcggggct
      361 cggtcatagg cagtcgtgac cggctctcaa cgaccgtccc gcagcgcctc gatccactgg
      421 cggtgctcgt cgtagtggcc ggcggtgttg cccatgatcc agtagaggat cgggtcgccg
      481 ttgtctttgc cgggctcgtc gggcaggtag tgggagtacg ggcggagcag atcatcccag
      541 gtgaaacggt tgaggatctc cagcgtctct tcgtgctgct gcagaagccg cgccagcacc
      601 tcgccagcgg agaggtcgcg gtgctgctcg aagatgatgg cgttgatccc gtcgtggccc
      661 aggttgagat agtcgtcctc actgacgccg agtgtctcgt gtcgcggctg gttcaacagc
      721 agttgtgcga tcgagcgttc ccagaggacg acgttcatca ggtgatcctt gatcgcccag
      781 ccgttcgcgt cgcgcacgtc gctgagttgc tgctcgtcga ggccggcaat ggcagcttca
      841 agcgcctgcc aggcgacgct gatgcgtcgg ttcagctcgt tcatgtcgcc ttcgataggc
      901 tgggactgct cctcctgcat gacaggctcc tcactcgtag agaaatgacg ggacgatcag
      961 gtcgccagcg cgagctgttt ggccggcttg gcgatctcga cgccctcgtg ggtcagcagg
     1021 gcggtcttga ggtgcaggcc gccgccgaat ccggtcagcg taccgtcggc gccgatgatc
     1081 cggtggcagg ggatgacgag cgggatgcgg ttcgtgccgt tcgcgcggcc aaccgcccgc
     1141 gacgcctgcg gccggccgag cgagcgtgcc agctcgccgt aggtgcgcgt ttcgccgaac
     1201 ggaacctgca tcagttcccg ccagaccgct ttctgccact cggtcccttg caggacaacc
     1261 ggcaggtcga acgtcgtccg ctcgccgctg gcgtactcga ccagttgctg gcgaacgtgg
     1321 gcggtccgct cagggtcgct gaggcaggtg tagccttcgt tgatcagatc ctcttcgatg
     1381 gttgtcgggt agtgcccttc caggaacgac aggaagagca gcgcgccgtc ctcgttgacg
     1441 gcgaaggtca gtggtccggc cggagaatcg acggtctcca ggtaggcgtt catcgtcgtt
     1501 cctttcgtga gatcaggcaa cggccccatg ccgctgccag agatgaaacg tcgccaggct
     1561 gcggtagggg ctgaagcggt ccatcagcgc cagcgtctcc agcttgccgg ggcgctcgtc
     1621 gagggcgaag aactgcttga ggccgctcgt cagcccggtg tcgcccagcg gcacgcagtc
     1681 gaggaagccg tagtggcgca tcatcaggta atgtgccgac cacgggccga tgccccggac
     1741 ggccagcagc gtccgctcga tccgcgtcgc ggaactgccg gccagcgcat cgagcggcag
     1801 ccgcccgtcg atgaccgagc gggctgcgcc gaccaggtag ctggccttgg cgcgcgagaa
     1861 gctgagcggc agcagatcct ccggctcaat cgcggcgacg gccgctaccg acggcggcgc
     1921 gtagagaccc tcaccgagcg gctggctgac gtgctcgatc aggcgatgcc gcagggtgta
     1981 ggcgaaggcg aggttgatct gctggccaac gatcgcccag accagcccgt cgaacgggtc
     2041 cgccagcagc ggcactcgca gcccgcgctg gccgtcaatc agcggcgcca gttccggcga
     2101 gccggtcacc agcgcctcga agcgggccgg gtcaaccgtc agccccagcg tcgccagcac
     2161 ccggctgtgc agcgcctcca tcagatcagg gttgggctgc tcgggaagaa gaaccgtgca
     2221 gcgcgccaag ccatcctcga agacgatccg caccagcgcg ggctgctcgc cggcccggta
     2281 ggcaatcagg caggtttggc cctcgacgcg ctcggtcagg ctgttcttgt cgcgcccgag
     2341 gtaggcgaga atccggccga gcgggtagtc atccggcagg cgcagatcga acgcggcctc
     2401 gtccggcagt tgccgatagc gggccggact catcgcggtg tacttgcgga aattctcgtt
     2461 gaaggccgac aggctctcga agccgacctc gaaggcgatc tcggcaaccg gccgctggcc
     2521 gtgcagcagc atccgccgcg ccgccgcgac ccgcag
//
LOCUS       DASUGJ010000787         3169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_127840, whole genome shotgun sequence.
ACCESSION   DASUGJ010000787 DASUGJ010000000
VERSION     DASUGJ010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127840"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1420
                     /locus_tag="VFV93_17790"
     CDS             209..1420
                     /locus_tag="VFV93_17790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5167264.1"
                     /translation="MRDIYERVLRRRPALVPVLLIFALSRLAFYAAAIPGAYLLPDAP
                     NQPAAVDVNARLALALHWRWDAVHYYTVSIDGYSEGGTTAFFPLLPLLMHVASFVLGG
                     FQAPAHLPIQQAETTTLLAGVLVAHAISLLAFWLLYQLASEELGDRDAAIRTVLYAAF
                     FPLAFHYGVPYTEGLLLATAAGTFLAARRRQWVRAGIWAGLGAASRSSGILLTPVLLL
                     EIGLAWRRGGLTRAEQPRALLGLLLAPLGLLLFMAELWNRTGDPFAFIEAQKAWGRES
                     TWPWTTLLRGIDYALHPAMTDRADVYMRGVMHLLITLAFLAILIGYARRWRPSYTLFG
                     GLLFVLILASPIEGQWTMHHLGRFLMPFFPIYFVLAGWGRHPWVDRAILATFLPLFGL
                     LTLLYVNWYAV"
     gene            complement(1425..2009)
                     /locus_tag="VFV93_17795"
     CDS             complement(1425..2009)
                     /locus_tag="VFV93_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5167265.1"
                     /translation="MAATYNSVEKNVLTTTSDYLFSWARRSSLWWLQFGLACCGMEMI
                     AAQMPRYDMGERFGMLYRASPRQADLMIVAGTVTRLMAPVVRTLYEQMAEPKWVLSMG
                     SCANVGGPFDTYAVVQGVDQIVPVDVYIPGCPPGPEALMQGVLDVQNRIIKYQKISKS
                     SGREVAEHVRNEDRLVAREQIAGTDATFLSSHFG"
     gene            complement(2225..3031)
                     /locus_tag="VFV93_17800"
     CDS             complement(2225..3031)
                     /locus_tag="VFV93_17800"
                     /inference="COORDINATES: protein motif:HMM:NF003969.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEX5167266.1"
                     /translation="MSTDLTSRQSLYAADPASVRAAIRAGEWTTQTAGLAPGYTQANL
                     VILPRDLAYDFLLFCVRNPKPCPIIDVTDVGSPVPAQAAPDADLRTDLPHYRVYEHGE
                     LVEEPTDILHRWQDDMVAFLLGCSFTFEHALVAGGVPLRHLDCGDGVPMYVTNRACFP
                     AGGFSGPLVVSMRPIRAEQVDLASDISSRYARAHGAPVHAGDPAALGIADLATPAWGD
                     PTEIRPGEVPVFWACGVTPQAVALHSKPPLVITHSPGHMFVTDIEIGALV"
BASE COUNT          513 a         1024 c         1090 g          542 t
ORIGIN      
        1 tccaccaatt gcgctcgtcg tcccacgccg ggttgcccca ttccccaatc ccaaagagcg
       61 gccgcgcacc atcatacggg attgtggggt ggagtgggaa tgcgggacgc gtcctgcgtc
      121 ccgccgttgc ctcacatgca cgggacaggc atccttccca acactgcgga acgagtagac
      181 gaacgaacgg cggctcgcaa cggaacggat gcgcgacatc tatgagcggg tgcttcgccg
      241 gcggccggcg ttggtgccgg tgctgctgat cttcgcgctc agccggctgg cgttttacgc
      301 ggcggcgatt cctggtgcgt acctgctgcc ggacgcgccg aaccagccgg cggcggtcga
      361 cgtcaacgcg cggctggcgc tggcgctgca ctggcgctgg gacgcggtcc actactacac
      421 cgtctcgatc gacggctaca gcgagggtgg gacgacggcc ttcttcccgc tcctgccgct
      481 gctgatgcac gtcgcctcgt tcgtcctcgg cgggttccag gcgccggctc atttaccgat
      541 ccagcaggcg gagacgacga ccctgctggc cggcgtactg gtggcgcacg cgatcagcct
      601 cctggcgttc tggctgctct accagttggc gagcgaggaa ctgggggacc gcgatgcggc
      661 catccgaacc gtgctgtatg ccgcgttctt cccactagcg ttccactacg gcgtgcccta
      721 cacggagggg ctgctgctgg cgacggccgc cgggacattc ctggctgccc ggcggcggca
      781 gtgggtacgg gccggcatct gggccgggct gggagcggcg tcacggtcaa gcggcatcct
      841 gcttacgccg gtgctgctgc tggagatcgg gctggcctgg cggcggggcg ggctgacgcg
      901 cgccgagcaa cctcgggcgc tgctgggact gctgctggcg ccgttggggt tgctgctctt
      961 catggcggag ctctggaacc ggaccggcga tccgttcgcg ttcatcgagg cgcagaaggc
     1021 atggggccgc gaatcgacct ggccgtggac gacgctgctg cgcggaatcg actacgcgct
     1081 ccacccggcg atgaccgacc gggccgacgt ctacatgcgt ggcgtcatgc acctgctgat
     1141 cacgctggca ttcctggcga tcctgatcgg ctacgcgcgg cgctggcggc cctcgtacac
     1201 gttgttcggc gggttgctgt tcgtcctgat cctggcctcg ccgatcgagg gccagtggac
     1261 gatgcaccac ttgggccgct tcctgatgcc gttcttcccg atctacttcg tcctggccgg
     1321 ctggggacga cacccgtggg tggatcgcgc gatcctggcg acgttcctgc cgctgttcgg
     1381 cctgctgacg ttgctctacg tcaactggta cgccgtctga cgcgctagcc gaagtggctg
     1441 ctcaggaagg tcgcgtcggt accggcgatc tgctcgcggg cgacgagccg gtcctcgttt
     1501 cgcacgtgct cggccacctc acggccggaa ctcttgctga tcttctggta cttgatgatc
     1561 cggttctgga catccagcac gccctgcatc aacgcctccg gcccgggcgg gcaacccggg
     1621 atgtagacat cgaccgggac gatctgatcg acgccctgga cgacggcgta ggtgtcgaat
     1681 gggccgccga cgttggcgca ggagcccatc gagaggaccc acttcggttc ggccatctgc
     1741 tcgtagaggg tgcgaacgac cggcgccatc aggcgggtga cggtaccggc gacgatcatc
     1801 agatccgcct ggcgcgggga ggcgcggtag agcatgccga agcgctcgcc catgtcgtag
     1861 cgcggcatct gggcggcgat catctccatc ccgcagcagg cgaggccgaa ctggagccac
     1921 cagaggctcg accgtctcgc ccagctgaac aggtagtctg atgttgtcgt gaggacgttc
     1981 ttctcgacac tgttgtaggt cgcggccata acgcagtccc ttcattcagg accggaacag
     2041 gccgacgacg tgccgggccg cgcggcccgt ccagtcactc gtctcaaccc caacctacgc
     2101 cccatccggc catccggcat cgtccgaaga cctagttccc tacctgtgcg atgggacagg
     2161 gcgcgtacaa caattgcgcg gatcgaacac atgtgttcca gacgaacagc ctcagcacgg
     2221 agcgctacac gagcgcgccg atctcgatgt cggtcacgaa catgtggccg ggagagtggg
     2281 taatgacgag cgggggcttc gagtggagcg cgacggcttg tggtgtcacg ccgcaggccc
     2341 agaagaccgg cacctcgccg ggacgaatct cggtcgggtc gccccaggcg ggagtggcga
     2401 ggtcggcgat gcccagcgca gccgggtcgc cggcatggac cggcgcaccg tgggcgcggg
     2461 cgtagcggga ggagatgtcg ctggcgagat caacctgctc ggcacggatc ggccgcatcg
     2521 agacgaccag cgggccgctg aacccgccgg ccgggaagca ggcgcggttg gtgacgtaca
     2581 tcgggacgcc gtcgccgcag tccaggtggc gcagcgggac accaccggcc accagcgcat
     2641 gctcgaaggt gaagctgcag cccagcagga aggcgaccat gtcgtcctgc cagcggtgga
     2701 gaatgtcggt cggctcctcg accagctcgc cgtgctcgta gacgcggtag tgcggtaaat
     2761 cggttcgcag gtcagcatcc ggggcagcct gagccggaac cggcgagcca acatcggtca
     2821 cgtcgatgat cggacagggc ttggggttgc gaacgcagaa gagcaggaag tcgtaggcaa
     2881 gatcacgcgg caggatgacc agattcgcct gcgtgtagcc cggcgccagc ccggccgtct
     2941 gtgtcgtcca ctccccagcc cggatcgcgg cgcggacgga cgctgggtca gcggcgtaaa
     3001 gcgactgtcg cgatgtcagg tcagtgctca tgtcagtctc cttcgcaatg aacgagcgac
     3061 gatctgccga tcagtgctct atcatccgac ccatatcttg gcgcagcggt cacaaccgac
     3121 ggatcttcgt gaaagagcat cgcaatgtct ggtgaagagc ataaggcaa
//
LOCUS       DASUGJ010000788         5456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1934713, whole genome shotgun sequence.
ACCESSION   DASUGJ010000788 DASUGJ010000000
VERSION     DASUGJ010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934713"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1305)
                     /gene="fabF"
                     /locus_tag="VFV93_17805"
     CDS             complement(58..1305)
                     /gene="fabF"
                     /locus_tag="VFV93_17805"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5167267.1"
                     /translation="MANRSADRVVVVSGIGCVSAVGVGREENWSAILDGCGGVGPIDR
                     FDAGEFPVRIAAQVGEFEPARYMDAKDARRADRFIQFAVAAAREAVEHAALTIDDDNA
                     ERVGVIIGTAMGGMATFEDGVRTLHTRGPGRISPFFVPMLLPDMATGIVSIQLGAKGP
                     NLATVSACASAAHAIGEAAATIQRGDADVMLAGGSEAAVTPAGVAGFAAAGALSTRND
                     DPAHASRPFDRQRDGFVLGEGSAVLVLESLDHAIARGATPLAVVSGYATTADASHIVQ
                     PSPGGEGAARAMTLAIARAGLRPSDISYINAHGTSTRLNDAYETMSVKTVFGAAPPPI
                     SSTKGATGHLLGAAGAVEAAYSVLTLRDQVLPPTINYEEPDPDCDLAYVPNTARRADV
                     EHVVSNSLGFGGHNVSLVFSRVR"
     gene            complement(1298..2257)
                     /locus_tag="VFV93_17810"
     CDS             complement(1298..2257)
                     /locus_tag="VFV93_17810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX5167268.1"
                     /translation="MLVAVTRRIDLGALAQLESIAELRYWDNDLPPSPEELRDLLAGV
                     DGALTLLTDRIDGGLLDELPNLKVVSNLAVGFDNIDVAACTARGVAACTTPNVLTQTT
                     AEFALALIFAVARQMVPGARAAREGEWKTWYPFRFLGRDLAGATLGVVGLGRIGRRLS
                     EMAAAIGMRVIYTDEYSDVPEFRRVSLEDLYRTADVISLHVPLTPETHGMIGADALAM
                     MKPDALLINTARGPVIDTEALVAALNDGLLGGVGLDVTDPEPLPPDHALYGFDRVTIV
                     PHIASATSVTRHKMSSLAAENIIAVLTGGEPPNCLNPEVLRRG"
     gene            2532..3593
                     /gene="holA"
                     /locus_tag="VFV93_17815"
     CDS             2532..3593
                     /gene="holA"
                     /locus_tag="VFV93_17815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX5167269.1"
                     /translation="MSYLLYGQNGLLLDERIAALRAELDPQNLSTTTIDVQTSSAGEI
                     ASACQAMPFFGGRRVVILRNPIQIPKRGDTTELEEDPEEAGGRVKWADLHTVLKATPD
                     TTDIIMRHDGSLAQGHYLVKATKALDWKLESFAARFGQELLGWIQQRVEREGMRIEPN
                     AVTRLAERLYPTSWQGGRWVNETPDMRLIASEVDKLCCAAIGGVITPATVDEMVADRS
                     GFTAFKLNDHLFSGRPTEALTELEHVLATGEPAERVLGQIGSEAISIATMKRSGGVPR
                     DALTAASGISANRIGGLQQKAAGISDPAIIAIAEEVRYSDASVKEGHQSSTSATIVPL
                     VAEVAEVVRRGSSAGRRRS"
     gene            complement(3590..3862)
                     /gene="rpsT"
                     /locus_tag="VFV93_17820"
     CDS             complement(3590..3862)
                     /gene="rpsT"
                     /locus_tag="VFV93_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX5167270.1"
                     /translation="MANSKSAEKRARTNERRRIRNRRFRSASRTLVRSAEQVIAQADE
                     EAAAGAVKNAISMLDRAARKGIIHPNNAARRKSRLMAKYNKLSAAS"
     gene            4059..>5456
                     /gene="lepA"
                     /locus_tag="VFV93_17825"
     CDS             4059..>5456
                     /gene="lepA"
                     /locus_tag="VFV93_17825"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX5167271.1"
                     /translation="MTDQKHIRNFSIIAHVDHGKSTLADRLLEHTGTLTSRQMMEQVL
                     DTMDLEREKGITIKARAVRMDFRAADGELYELNLIDTPGHVDFSYEVSRSLAACEGAL
                     LVVDAAQGIEAQTIANVYLALENDLALIAVINKIDLPNAMPEKVSNEVGDLIGLLPDE
                     IIPASAKEGKGVPEILQALVDRIPPPAGTEKAPLRALIFDSHYDAYKGVIAYVKIVDG
                     VLDTDSRIRIMATGKEAELLEIGVFRPNMTPVKQLGTGEVGYVATGLKVVRDCQVGDT
                     ITLAASPASEPVPGYRPVKPMVFAGFFPVNNEDYIELRDALDRLQLNDASLVFEPESS
                     DALGFGFRCGFLGLLHMEIVQERLEREYNLDLLATAPSVEYEVLKMTGETILVDNPSD
                     LPDPGVIEEIREPWMDITIISPARYIGTVMDLITNRRGNFDSMDYLDEDRVSLKFGMP
                     LGELIVDFYDQLKSRT"
BASE COUNT         1004 a         1761 c         1747 g          944 t
ORIGIN      
        1 tatcgcccgt accggattga ggtattggga ttggaggaac gacgttgtgg caactggtta
       61 ccgaacccgc gagaagacca acgacacgtt atgtccaccg aagccgagcg agttgctgac
      121 gacgtgctcg acgtcggctc gtcgggccgt gttgggcaca taggcgagat cgcagtctgg
      181 gtcgggttct tcgtagttga tggttggcgg gaggacctga tcgcggagcg taagcaccga
      241 gtaggccgct tcaactgcgc ccgccgcgcc gagcaggtgg ccggttgcgc ctttggttga
      301 tgagattggg ggcggagccg ctccgaagac ggtcttgacc gacatcgtct cgtaggcgtc
      361 gttcaggcgc gtcgaggtgc cgtgggcgtt gatgtacgag atatcgctcg gcctcaaccc
      421 ggccctggcg attgccagcg tcatcgcccg ggctgcgccc tccccgcccg gcgagggctg
      481 gacgatgtgc gaggcgtctg ctgtcgtcgc gtagccgctg acgaccgcca gcggagtagc
      541 accgcgtgcg atggcgtggt caagcgattc caggacgagg acggccgaac cctcgccgag
      601 gacgaagccg tcgcgttgcc ggtcgaacgg gcggctggcg tgggcaggat cgtcgttacg
      661 ggtcgagagg gcgccggcag ccgcgaagcc tgctacgccg gccggcgtga cagccgcttc
      721 ggacccgcca gccagcatga cgtcagcgtc accgcgctgg atcgtcgcgg cggcctcacc
      781 gatcgcgtgg gccgcgctgg cgcaggcgct gacggttgcc agattgggcc ccttcgcgcc
      841 aagctgaatc gagacgatgc cggtcgccat gtccggcagc agcatcggaa cgaagaacgg
      901 gctgatgcgt ccggggccgc gcgtgtgcag cgtgcggacg ccgtcctcga aggtcgccat
      961 cccgcccatc gccgtgccga tgatgacgcc gacccgctcg gcgttgtcat cgtcgatcgt
     1021 gagagcggcg tgctcgaccg cctcgcgggc agcagcgacg gcgaactgga tgaaccggtc
     1081 cgcccgacgg gcgtctttgg cgtccatgta gcgggccggc tcgaactcac cgacctgggc
     1141 ggcgatccgg acgggaaact cgcctgcgtc gaaccgatcg atcgggccga cgccaccgca
     1201 gccatcgaga atcgcggacc agttctcctc gcggccgacg ccgacggctg agacgcaacc
     1261 gatgccgctg acgacaacca cgcgatccgc cgatcgatta gccacgacgc aacacctccg
     1321 gattcaggca attgggcggc tctccgccgg tcaggacggc gatgatgttc tcggcggcca
     1381 gtgacgacat cttatgtctc gtcaccgacg ttgcgctggc gatatgcggc acgatcgtga
     1441 cgcgatcgaa gccgtagagg gcgtgatccg gcggtagcgg ctccggatca gtcacatcca
     1501 gcccaacgcc accgagcaat ccgtcgttga gggccgcgac cagcgcctcg gtgtcgataa
     1561 caggcccgcg cgccgtgttg atcagcagtg catccggctt catcatcgcc agcgcgtcgg
     1621 ctccgatcat gccgtgcgtt tcgggcgtca gcgggacgtg cagcgagatc acgtcagccg
     1681 ttcgatagag gtcttcgaga ctcacacggc gaaactcagg aacgtcgctg tattcgtcag
     1741 tgtaaatcac ccgcatgccg atcgctgccg ccatctcgga aagtcgccga ccaatgcgcc
     1801 ccagcccgac gacgccgagt gtcgcgccgg ccaggtcgcg tccgagaaac cggaatgggt
     1861 accaggtctt ccactcgccc tcgcgtgccg ctcgcgcgcc cggaaccatt tgtcgcgcga
     1921 cggcgaagat caacgccagc gcgaattcgg cggtcgtctg ggtcagaacg ttcggcgtcg
     1981 tgcaggccgc gacgccccgc gccgtgcagg ctgcgacgtc gatgttgtcg aagccgacag
     2041 ccagattcga aacgaccttc aggttcggca gctcgtcgag caagcctccg tcgatgcgat
     2101 cggtcagcag cgtcagcgcg ccgtcgacac ccgccagcag gtcacgcaac tcttccgggg
     2161 acggcggcag gtcgttgtcc caatagcgaa gctctgcgat cgattcgagt tgcgcgagcg
     2221 cccccaggtc gattctgcgt gtcacggcca ccagcacagt gttacccctc cctcgttggt
     2281 gcgagtgtac gtgtccgtgc gggtgtacgg ccagtctggt agacttggat tgggtgagac
     2341 gagaaacgca gccgtgtgtt cctaatacgt ttcgagccaa cgtcactagt tttcgggtgc
     2401 ctttggacga acccaagggt tcgctcctcc gagtacccac ctgcgaacgc aggcttgaaa
     2461 ctagttcaca cggtgtcagc gcgtctcgcc cactcttttt tcctcccaca tgcggatgca
     2521 gggctggtga catgagctac ctgctctacg gacagaatgg cctgctgctc gacgagcgca
     2581 tcgctgctct gcgcgcggaa ctggacccgc agaatctgag cacaaccacc atcgacgtcc
     2641 agacatcgag cgccggcgag atcgcgtccg cctgccaggc gatgccgttc ttcggcggtc
     2701 gacgcgtcgt catcctccgc aacccgatcc agattccgaa acggggagac accactgagt
     2761 tggaagaaga ccctgaagag gccggcggcc gcgtcaagtg ggcggatctc cacaccgtcc
     2821 tgaaagcgac gccggacacg accgacatca tcatgcggca cgatggctca ctggcgcagg
     2881 ggcactatct cgtcaaggcg acgaaggcgc tcgactggaa actcgagtcg ttcgccgcac
     2941 gcttcggcca ggagttgctg ggctggattc agcagcgtgt cgagcgtgag gggatgcgaa
     3001 tcgaaccgaa cgccgttacc aggctcgctg aacgcctgta tccaacgtcg tggcagggtg
     3061 gccgttgggt caacgagacg cctgacatgc gcctgatcgc ttcagaggtc gacaagctct
     3121 gctgcgcggc catcggcgga gtgatcacgc ccgcaacggt cgacgagatg gttgcggacc
     3181 ggagcggatt caccgccttc aagctcaacg accacctctt cagtggccgt ccaaccgagg
     3241 cgctgaccga gcttgaacac gtcctggcga ccggcgaacc agccgaacgt gtgctggggc
     3301 aaatcggcag cgaggcgatc tcgattgcga cgatgaagcg gtcgggcggc gtgccacgcg
     3361 acgccttgac ggccgcatct ggcatctccg cgaaccgcat cggcggattg caacagaaag
     3421 ccgccggcat cagcgatcca gcgatcatcg caatcgctga ggaggtcaga tattcggacg
     3481 cgtccgtgaa agaggggcac cagagcagca cctcggcaac gatcgtcccg ctggtcgcgg
     3541 aagttgccga ggtcgtgcgg cgtgggagca gcgccgggcg gcgacgctcc tagctggcgg
     3601 cggagagctt gttgtacttc gccatcagcc gcgacttacg gcgcgcggcg ttgttcgggt
     3661 ggatgatgcc cttccgggcg gcccgatcga gcatgctgat ggcgttcttg acggcgccgg
     3721 cagcggcctc ttcgtcggcc tgcgcgatca cttgctcagc cgaccgcacc agcgtccggc
     3781 tggccgaccg aaaacggcgg tttcgaatgc ggcgccgctc gttcgtcctg gcgcgcttct
     3841 cggcagactt ggaattggcc aacgcttgtt tctccctcga cctgcgtgaa catgacaaaa
     3901 gcgcccgacc atcgcgggcg tggcaacctt gctattctac gcgaatccgc gccacaatcg
     3961 caacatcgcc tggcacaagt cgggatgccg gctccagtat acttactcac ttcgggcagg
     4021 cgacgatgcc cgctcgcttc agcatgcatt gagaacagat gactgatcag aaacacattc
     4081 gcaatttcag catcatcgcc catgtcgatc atggcaagtc cacgctggcc gatcgcctgc
     4141 tcgaacacac cggtaccctt accagccggc agatgatgga acaggtgctc gacacgatgg
     4201 acctcgaacg tgagaagggc atcacaatca aggcccgtgc cgtccggatg gacttccgcg
     4261 cggccgacgg cgagctctac gagctgaacc tgatcgacac ccccggccac gtcgacttct
     4321 cctacgaggt cagccgctcg ctggccgcct gcgagggcgc cttactggtc gtcgacgccg
     4381 cccaggggat cgaggcgcag acgatcgcta acgtctacct cgcgctggag aacgacctgg
     4441 cgctgatcgc cgtcatcaac aagatcgacc tgccgaacgc gatgccggaa aaagtctcca
     4501 atgaagtcgg cgacctgatc ggcctcctgc cggacgagat catcccggcc tccgccaagg
     4561 agggcaaggg cgtcccggag atcctgcagg cactggtcga tcggattccg ccgccggccg
     4621 gcacggagaa ggctccgctg cgggcgctga tcttcgattc gcactacgac gcctacaagg
     4681 gcgtcatcgc ctacgtgaag atcgtcgatg gcgtcctcga caccgattcg cgaattcgga
     4741 tcatggcgac cggcaaggaa gctgagctgc tcgagatcgg tgtcttccgg ccgaacatga
     4801 cgcccgtcaa gcaactcggc accggcgagg tcggctacgt cgcgaccgga ttgaaggtcg
     4861 ttcgtgactg ccaggtcggc gacacgatca cactggcagc cagtccggcc agcgaaccag
     4921 tgccaggcta ccggccggtc aagccgatgg tctttgccgg gttcttcccc gtcaacaacg
     4981 aggactacat cgaactgcgt gacgctctcg atcggctgca actcaacgat gcctcgctcg
     5041 tctttgagcc ggaatcgtcg gacgcgctcg gcttcggctt ccgctgcgga ttcctcgggc
     5101 tgctgcacat ggagatcgtg caggaacggc tggaacgcga atacaacctc gacttgctgg
     5161 cgacggcccc gagcgttgaa tacgaagtcc tcaagatgac gggcgaaacc attctggtgg
     5221 acaatccctc cgatctgccc gatcccggcg tcatcgagga gattcgcgag ccgtggatgg
     5281 acatcacgat catctcaccg gctcgctata tcgggacggt catggacctg atcacgaacc
     5341 ggcgcggtaa cttcgactcg atggactacc tcgatgagga tcgcgtctcg ctcaagttcg
     5401 ggatgccgct cggcgagctg atcgtcgact tctacgacca gctcaagtcg cgcacg
//
LOCUS       DASUGJ010000789         9246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_824462, whole genome shotgun sequence.
ACCESSION   DASUGJ010000789 DASUGJ010000000
VERSION     DASUGJ010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824462"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFV93_17830"
     CDS             complement(<1..452)
                     /locus_tag="VFV93_17830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5167272.1"
                     /translation="MQIVVIGAGVLGSSLAFRLAQAGQRVTLVERAWVAHGTTGSTFA
                     WINSNQKIPEDYYALNLAGVRAHRELRDELGAAPWLNEGGNLFWFAGGEGANELEGRV
                     ARLQNWGYPAEWIGREAARELEPKLTPEPEVEQIAYFPEEAWINGPGLA"
     gene            complement(565..1206)
                     /locus_tag="VFV93_17835"
     CDS             complement(565..1206)
                     /locus_tag="VFV93_17835"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5167273.1"
                     /translation="MSSYPTHDWLSPLAVESLGDFHLTRQIDADLSQRALDAAGDPAA
                     RDALFNLLAFKINRFCSRFRRWNLRPWEFEDVRQEAYLAFVDVLNGWQPVPGREVPAG
                     FGYYFMRVYPLRLTDRVRRILRTKRNRPATTSLTDADVDRRDPAEMERDIETLASIIQ
                     LSEGLDAADARILLLRTTDDLSPDEIAAQAGVSRRTYFRRWNGIAATIRREAG"
     gene            complement(1369..1668)
                     /locus_tag="VFV93_17840"
     CDS             complement(1369..1668)
                     /locus_tag="VFV93_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167274.1"
                     /translation="MTERTTAAGRKRATKQRWECRCADPPILLAMYEPGGQVEIKLRD
                     RFYLASGCVHATCPRCGTQHILDLRPSSGPDDGCPDPEQALPFPPTGGSSNGGWF"
     gene            complement(1711..2910)
                     /gene="dinB"
                     /locus_tag="VFV93_17845"
     CDS             complement(1711..2910)
                     /gene="dinB"
                     /locus_tag="VFV93_17845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX5167275.1"
                     /translation="MEVRGRQRWVIHADLDAFFAAAEVLRRPELAGLPVVVGGAPEGR
                     GVVAAASYPARAFGVRSAMPMAEALRLCPDLVIVRGDHHYYRELSHQFRTILDAFSPL
                     VEVVSIDEAYLDASDSDRLFGGAEALAVALKRRVRDEIGLTVSLGVAGNKLVAKIASD
                     LDKPDGLRVVRPGQEAATLAPLPIERLPGVGPKSTARLREGGIQTLGQLAVAPDVLLR
                     AVAGNDAERLRARARGEDDRPVVPAWEREPRKSIGHEETFGRDLRGLPELDAPLYHLS
                     AATGSELRREGLTGTTVTLKLRYSDFTTITRQASLLKASDAHQEIYQLARTLLSRALA
                     ERNSPVRLLGVRMTGLASARQLALFDDADERTRKLNAAIDQLAAKGGERLVVPARFAA
                     PRRRPSL"
     gene            3148..5718
                     /locus_tag="VFV93_17850"
     CDS             3148..5718
                     /locus_tag="VFV93_17850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5167276.1"
                     /translation="MSSIAINNRPVTERVWLSLILTDRATQSLILFGAMLTALTVLGP
                     PSGDLGKITSNALFIITSGGATLFCVRTARRLGNAGLPWLAFAAGSGCWFLGQLVWSW
                     YDVILWTAPPYPSLADVGYSLFYPFMFGGLTLLIRQRIRDLPLGEVLLDSLIVVVAFA
                     LVAYELFVAPLTGSSGLAAPELVITLGWEVVTFGLVLLAGIALFIRADLLMRGPLSIL
                     LLGFIAFTASNVAYGQQALDDTYTGGSLTDLGWQLGFIAIASAAQVALRSAMPSRQID
                     HARDRRASLARIVMMVGSVLIVTAMAAYAAAQPRAEPAVIVLVLLAGTLLAVRLGYAA
                     LQTEHLIRRTRERDRLAGVVSASSAISSTLDLDELLPLLATAAKDAVGRARGEVYVFS
                     EDMSVVEAGAHCGFSESDAARLASLLEQPVGAYVAERRVIETLKPTLQVIDEPGIPDA
                     DVAMFAELGKLHSLITPLIAHDRVVGVVDTWTPLDTTPFEPADIAAAAAIGQQAGLAI
                     HNARLLAGTRQHAAEQEALLRVTQAAVSSLDLDAVLAEIAQASLGVANAEACAIEIWD
                     RERGDSVLAAEATIDAWPGTSAVGKRLSFDDWPISPRVLHEQVTLNLLATDDILSPRE
                     QEHLRADDTNSMLMVPLVVNGVSLGILNLFSRNPRRFTRDEIRLASDLAAQISIAIDR
                     ARLHEALRERADTDGLTGLLNHRAILEQLDAETARARREAPPLSILMIDLDGFKQVND
                     TYGHLTGDQLLRETAAFLRAGIRDIDQAGRYGGDEFLLLLPNTDNDDARQLASRLLDL
                     AEDASLTIHGKHFPIQISIGIATTPDDGLTRQELIAVADTRMYEAKSARRWIVAGAQG
                     "
     gene            complement(6102..7859)
                     /locus_tag="VFV93_17855"
     CDS             complement(6102..7859)
                     /locus_tag="VFV93_17855"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX5167277.1"
                     /translation="MIPVWLAAALRGDNRWVRVALHLVAIAGLVGTLLLVPVAEGFYT
                     RGVATGRAWHPIPHTDVHRMGINTFLNEEPDPAVVERSLDMIRDGGYGYIRQIFGWYE
                     IEPQPGVYVDAEGRSTWAKYDRIVDEATARGLQIIARLEKPPPWAKAGQPHPEIDGPP
                     DRLSDYANFVEQVVTRYRGRLTYVQLWNEPNLEGEWGANPIDPRGYVELLAAGAEAAR
                     KANPDVVILLAGLAPTDQTGPANLSDLLFLDQVYELGGAAYFDIVSVMVYGYGYPPWD
                     RRVSFERNNFSRPIQTREIMVRHGDEDTPVWAVEYGWVSLPDDWSGDPSPWGEPVSEA
                     QQAVYLVDGYLRAQREWPWMGVMAVWAFRFPHAPDAPDQLGNPTQGFALVAHDFTPRP
                     AYLALQQHADRIRASGTGSHQLTAAQQAQIAAGAPVDLVVAGERVDLLVSGAGQVERI
                     VDGRLRETLAFSAEDGERTRVTVARGMRETAHTISLRISGQPDGPPPQVIGYVVSRIW
                     FHSWIYPWLTGVLLLVLAGNVASLGWAVWDWWRASTDERSIVAEQKAHQCETDIVEID
                     SEGSDDEHDSARLTRSNHG"
     gene            8183..>9246
                     /gene="apbC"
                     /locus_tag="VFV93_17860"
     CDS             8183..>9246
                     /gene="apbC"
                     /locus_tag="VFV93_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEX5167278.1"
                     /translation="MATFTEDQIIEAIRPVQDPDLGRSLVELNMVKGVEIDGGRIKIN
                     VELTTPACPLRGKIEDDVRAAAAAVPGVEDVAVAFTSRVRPSGKGMPDKTPLTGVKNV
                     IAVASGKGGVGKSTVAVNLAASLARDGASVGLLDADVYGPSIPLMMGTSAKPIMQNNK
                     IVPLEAFGVKIMSIGFLLEADRALIWRGPLVAQLINQFLNEVAWGELDYLVIDLPPGT
                     GDVQLTLVQKIPLSGAVIVTTPQDVALADAIKGIQMFQEVKTPILGIIENMSFFICPS
                     CGEQTEIFGFGGGERTAERYNVPLLGRIPLDTAVRQGGDIGHPITVSEPESPPGEAFR
                     DAARQCARQLAIDAVEKPRRA"
BASE COUNT         1519 a         3225 c         2997 g         1505 t
ORIGIN      
        1 gccaggccgg ggccgttgat ccaggcttcc tcggggaagt aggcgatctg ctcgacctcc
       61 ggctcgggcg tcagcttcgg ctccagttcg cgggccgcct cgcggccgat ccactcggcc
      121 gggtagcccc agttctggag acgggcaacg cggccctcca gctcgttggc gccctcgccg
      181 ccggcgaacc agaacaggtt gccgccctcg ttgagccacg gcgccgcacc cagctcgtcg
      241 cgtagctccc gatgggcgcg cacgccggcc aggttcagtg cgtagtagtc ctcgggaatc
      301 ttctggttgc tgttgatcca ggcaaaggtc gagccggtcg tcccatgcgc cacccaggcc
      361 cgctcaacca gcgtcacccg ctgccccgcc tgcgccaacc ggaacgccag cgacgatccc
      421 agcacaccgg cgccgatgac cacgatctgc atctgaaatc actcctcgct acccagccaa
      481 tgccgcaatc catgcgtcgg ggtgattcac cgatatcgtc ccgacgcatg gattgcggca
      541 gtggctgggc gcatcactcg cgcctcaccc cgcttcgcgc ctgatcgtag cagcgattcc
      601 gttccagcgg cgaaagtagg tgcggcggct gacgccggcc tgggcggcga tctcgtccgg
      661 ggaaaggtcg tcggtcgtgc gtagcagcag gatacgcgcg tcggcggcgt cgagcccttc
      721 tgaaagctgg atgatcgacg ccagcgtctc gatgtcccgc tccatctcgg ccgggtcgcg
      781 ccggtcgacg tcggcatcag tcaggctggt cgtcgccggg cggttgcgct tggtgcgcag
      841 gatgcgccgg acgcggtcag tcagccgcag cgggtagacc cgcatgaagt agtagccgaa
      901 gccggccggt acctcccggc cagggaccgg ttgccaaccg ttgagaacgt cgacgaaggc
      961 caggtaggcc tcctggcgga catcctcgaa ctcccaagga cgcaggttcc agcgccggaa
     1021 gcgggagcag aagcggttga tcttgaaggc gagcaggttg aacagcgcat cgcgggcggc
     1081 aggatcgccg gcggcgtcga gcgcccgttg tgagagatcg gcgtcgatct gccgcgtgag
     1141 atggaagtcc cccagcgact cgacggcaag cggcgagagc caatcgtgcg tgggatagct
     1201 ggacatggcg cgctccacgg accagccggt ccgggcgaca gcggggtcac gacgcagaaa
     1261 cgcgcggggc cacgcatgcc agccggaccg accggcggtg cggcgcttcg gcgctctgca
     1321 ggatgaggca gcatcgcgtg ctgtgggagg agggcatgcc ggttccggct agaaccagcc
     1381 gccgttgctg gagccgcccg ttggcgggaa tggtagcgcc tgctccgggt ccggacagcc
     1441 atcatccggg ccggaggacg gtcgtagatc gaggatatgc tgggtgccgc agcgcgggca
     1501 ggtcgcgtgg acacagcccg acgcgaggta gaagcggtca cgcagcttga tctccacctg
     1561 cccgcccggc tcgtacatcg ccagcaggat cggcggatcg gcacagcggc actcccagcg
     1621 ttgcttggtc gcgcgtttgc gcccggctgc ggtcgttcgt tccgtcattc gtctcacccc
     1681 ctttctcgtt gtctggtcat cggccgcgca tcacaggctc ggccgccggc gcggtgcggc
     1741 aaagcgggcc gggacaacca gccgctcgcc gcccttcgcc gccagttggt cgatcgcggc
     1801 attcagcttc cgcgtccgtt cgtcggcatc gtcgaacagc gccagttgcc gcgccgaggc
     1861 cagccccgtc atccggacgc caagcagccg gaccggacta ttgcgctcgg ccagcgcccg
     1921 actcagcagc gtccgcgcga gctggtagat ctcctggtgc gcgtcactcg ccttcagcag
     1981 cgatgcctgg cgggtgatcg tcgtgaagtc gctgtagcgc agcttgaggg tgacggtcgt
     2041 gccggtcagt ccttcgcggc gcaactcgga gcctgtcgcc gctgacaggt gatagagcgg
     2101 agcgtccagc tcaggcaggc cacgcaggtc ccggccgaac gtctcctcat ggccgatcga
     2161 cttcctcggc tcccgttccc atgcgggcac gaccggacgg tcgtcctcgc cgcgcgctct
     2221 ggcccgcagt cgctcggcat cgttgccggc gaccgcccgt agcagcacat ccggcgcgac
     2281 ggccagctgg cccaacgtct ggatgccacc ctcgcgcagg cgtgcggtcg acttcgggcc
     2341 gacgcccggt agccgctcga tcggcaaggg cgccagcgtc gccgcctcct gtccgggccg
     2401 gaccacccgc aggccgtccg gcttgtcgag gtcggaggcg atcttcgcca ccagcttgtt
     2461 gccggccacg ccgagcgaga cggtcagccc gatctcgtcg cggacacgcc gcttcagcgc
     2521 gaccgccagc gcctcggccc cgccgaagag ccggtcggag tcgctggcat ccagataggc
     2581 ctcgtcgatg ctgacgacct cgaccagcgg cgagaacgcg tccaggatgg tccggaactg
     2641 gtgcgacagc tcgcggtagt agtggtgatc accgcgaacg atcaccagat cgggacacag
     2701 ccgcagcgcc tcggccatcg gcatcgccga gcgaacgcca aacgcccgcg ccgggtagct
     2761 ggcggcagcg acgacgccac gcccctccgg cgcaccaccg acgacgaccg gcaggccggc
     2821 cagctccggc cggcgcaaca cctcggcagc cgcaaagaag gcatccaggt ccgcatggat
     2881 gacccaccgt tgtcgcccgc gaacctccac tggcgccccc ttcagccaac cgctcgaatg
     2941 aaatagaaca tatgttctat ttctgtgtcg atcttagaac gctgttcgtt cgctgtcaat
     3001 cctgttcccg atagtcacac cggcccaacc aaccggatag ctccggcaat cgactgacta
     3061 ccagagattg ggatgtcttt cccattggga actgctgtgc cagtcggtag gatgactcgt
     3121 cagcagctgt ggatcgagga tacacgcgtg tcgtcaatcg caatcaacaa tcgaccggtg
     3181 acagagcgcg tctggctatc gctgatcctg acggaccgcg cgacccagag cctcatcctg
     3241 ttcggcgcga tgctgacggc cctgacagtg cttggcccgc cgtccggcga cctcgggaag
     3301 atcacctcca acgcactctt tatcatcacc tccggtggag ccacgctgtt ctgcgtgcgg
     3361 accgcgcgcc ggttgggcaa cgcggggctg ccgtggctgg cgttcgcggc cggcagcggc
     3421 tgctggttcc tcggccaact ggtctggagc tggtacgacg tcattctctg gacagccccg
     3481 ccgtatccgt cgctggcgga tgtcggctac tcgctgttct acccattcat gttcggcggt
     3541 ctgacgctgc tgatccgcca gcggatccgc gatctcccgc tcggtgaggt gctgctcgac
     3601 agcctgatcg tcgtggttgc cttcgcgctg gtcgcctacg agctgttcgt tgcgccgctg
     3661 accggctcca gcggcctggc agcgcccgag ctggtcatca cgctcggctg ggaggtggtg
     3721 acgttcggcc tcgtcctgct ggccggcatc gcgttgttca tccgcgccga cctgctgatg
     3781 cgtgggccgc tcagcatcct cctgctgggc ttcatcgcct tcaccgcatc caacgtcgcc
     3841 tatggccaac aggcgctcga tgacacctac accggcggca gcctgaccga tctgggctgg
     3901 cagcttgggt tcatcgcgat cgcgagcgca gctcaggtcg cgctgcgctc ggcaatgccg
     3961 agtcgccaga ttgatcacgc cagagaccga cgcgcgtccc tcgcgcggat cgtgatgatg
     4021 gtcggcagtg tcctgatcgt tacggctatg gcagcctacg ccgcggccca gccacgcgcg
     4081 gaaccggccg tcatcgtcct cgtcctgctg gccgggacac tgctggcggt ccggctcggc
     4141 tacgcggcgc tccagaccga acacctcatc cgtcgcacac gcgagcgcga ccggctggcc
     4201 ggcgtcgtct cggcgtccag cgcgatcagt tcgacgctcg acctggatga gcttctgcca
     4261 ctcctggcaa ctgccgctaa ggatgcggtc ggtcgggcgc gtggcgaggt ctatgtgttc
     4321 tctgaggaca tgtcggtggt cgaagccggt gcgcactgcg gcttcagcga gagcgacgcc
     4381 gctcgccttg cctcgcttct cgaacaaccc gtcggtgcct acgtcgccga gcggcgcgtc
     4441 atcgagacgc tcaagccgac cctgcaagtg atcgacgaac ccggcatccc ggatgccgat
     4501 gtggcaatgt tcgcggaact cgggaagctc cattcgctga tcacgccgtt gatcgcccac
     4561 gaccgggtcg tgggcgtcgt cgatacctgg acgccgctcg acacaacgcc gttcgaaccg
     4621 gccgatatcg ccgctgccgc cgcgatcggc cagcaggccg ggctggcgat ccacaacgcc
     4681 cggctgctgg ccggcacccg gcaacatgcc gccgaacagg aagcgctact gcgcgtcacc
     4741 caggcggccg tatccagtct cgacctcgat gccgttctgg ccgagatcgc ccaggctagc
     4801 ctcggtgtcg ccaacgccga agcctgcgcg attgagatct gggaccgcga gcggggcgat
     4861 agcgtcctgg ctgctgaggc cacgatcgac gcctggcccg gcacgtccgc cgtcggcaag
     4921 cgcctgtcgt tcgacgactg gccgatctcg ccgcgtgtgc tgcacgagca ggtcacgctg
     4981 aacctgctgg cgaccgatga catcctcagc ccacgcgagc aggaacatct gcgcgccgac
     5041 gataccaaca gcatgctgat ggtgccgctg gtcgtcaacg gcgtctcgct cgggattctg
     5101 aatctcttct cgcgtaatcc acgccgcttc acccgcgacg agatccggct ggcgagtgac
     5161 ctggccgcac agatctcgat cgcgatcgac cgcgcccggc tgcacgaagc gctccgcgag
     5221 cgggccgaca cggacggact gactgggctc ctcaaccacc gcgcgattct cgagcagctg
     5281 gacgccgaaa cggcgcgcgc ccggcgggag gctccacctc tctcgattct gatgatcgac
     5341 ctcgacgggt tcaagcaggt caacgacacc tacggccatc tgaccggcga ccagctgctg
     5401 cgcgagacgg ccgccttcct gcgcgccggc atccgcgaca tcgaccaggc aggccgctac
     5461 ggcggcgacg agttcctgct gctgctgccc aacaccgaca acgacgacgc tcgccagctc
     5521 gccagccgcc tgctcgacct ggccgaggac gcgagtctca cgatccacgg caagcacttc
     5581 ccgatccaga tctcgatcgg catcgcgacg acgcccgacg acggcctcac ccgccaggag
     5641 ctgatcgccg tcgccgacac gcgcatgtac gaggccaaat ccgcccgccg ctggatcgtc
     5701 gccggtgctc aggggtagcg cgtgatgcgt gatgcggtgt tgtccccccg tagaggccga
     5761 tcctgtatcg gccagtgccg tgaggcaccc gttggccacc acgcacgttt cggacagttc
     5821 ccccaacgcc tgcgcccata gatcaacccc gcctacgggt aaccacgcgc ctcacggcgc
     5881 tggccgatac gggatcggcc cctacgcggg gtgggcgatc tcctcgccag cgacgacgga
     5941 tcgagcgtat tccagacaca ctcagaacat catcaggcga gagatgcggg tacgcttcaa
     6001 gcaaatcgtc agtgtccaac gtcgatacga ggtgttcaag caccagttca actgagatcc
     6061 gtgtcccctt gacgattggc ttcccggcaa gaacggccgg atcaaccgtg attcgatctc
     6121 gtaagtcgtg cggaatcgtg ttcatcgtca gagccttccg aatcgatctc cacgatgtca
     6181 gtctcacatt gatgcgcttt ctgctcggca acgatagagc gttcatcggt gcttgccctc
     6241 caccaatccc agaccgccca cccaagcgag gcgacgttgc cggccagcac gagcagcagg
     6301 acgccggtca gccaggggta gatccagcta tggaaccaga tccgggaaac gacatagccg
     6361 atcacctgcg gcggcgggcc atccggctgg ccgctgatgc ggagcgagat ggtgtgagct
     6421 gtctcgcgca tgccgcgcgc gaccgtgacg cgcgtgcgct cgccgtcttc ggccgagaat
     6481 gccagcgtct cgcgcagccg gccgtcgacg attcgttcga cctggccggc gccggacacg
     6541 agcagatcga cccgttcgcc ggccacgacc aggtcaaccg gcgcgccggc tgcgatctgg
     6601 gcctgttggg cggccgtcaa ctggtgggag cccgtgccgc tggcgcggat gcggtccgcg
     6661 tgctgctgga gagcgaggta ggcgggacgc ggcgtgaaat cgtgtgccac cagcgcgaat
     6721 ccctgggtcg ggttgccgag ttggtcgggc gcgtcgggag cgtgtggaaa ccggaaggcc
     6781 cagacggcca tcacccccat ccagggccac tcgcgctgcg cccgcagata gccgtcgacc
     6841 agatacaccg cctgctgcgc ttcgctgacc ggctcgcccc acggcgacgg gtcaccgctc
     6901 cagtcgtccg gcagcgacac ccagccatac tcaaccgccc agaccggcgt gtcctcgtcg
     6961 ccatgccgga ccatgatctc gcgcgtctgg attggccgcg agaagttgtt gcgctcgaaa
     7021 ctcacgcgcc ggtcccaggg cgggtagccg tagccgtaga ccatcaccga gacgatgtcg
     7081 aagtaagcgg ccccgccgag ctcgtagact tgatccagaa agagcaggtc gctgaggttc
     7141 gccggtccgg tctggtcggt gggcgccagt ccggccagca ggatcaccac gtctggattc
     7201 gccttgcgcg cagcctcggc accggccgcc agcagctcga cgtagccgcg cgggtcgatc
     7261 gggttcgcgc cccactcccc ctccaggttc ggctcgttcc agagctggac gtaggtcagc
     7321 cgcccccggt agcgagtgac aacctgctcg acgaagttgg cgtagtcgga gagccggtcg
     7381 ggcgggccgt cgatctccgg gtgcggctgg ccggccttcg cccagggcgg cggcttctcc
     7441 agccgggcga tgatctgaag gccgcgcgcg gtcgcttcat cgacgatgcg gtcgtacttc
     7501 gcccaggtcg agcgcccctc ggcgtcgacg tagacgcccg gctgcggctc gatctcgtac
     7561 cagccgaaga tctggcggat gtagccgtag ccaccgtcgc ggatcatgtc cagcgagcgc
     7621 tcgacgacgg ccggatccgg ctcctcgttc agaaaggtgt tgatccccat ccggtggacg
     7681 tcggtatgcg gaatcgggtg ccaggcccgg ccggttgcga cgccccgtgt gtagaacccc
     7741 tcggcgaccg gcaccagcag caacgtcccg accaacccgg cgatcgcgac cagatggagc
     7801 gcgacccgga cccatcgatt gtcgccacgc agggcagcag cgagccagac aggaatcaca
     7861 ggcgagactc agcagggatc gtgctcccgg ctatccgaac ggacgcgcgg gcacgacgcg
     7921 cggcatagag ctggttcgcg gcatcgtcga tcatggccct gagtgtatcg cgtgcatcgt
     7981 tggtcatccc gaatggagcc agccatccag gttgctgagc aaccattcgg ttcttcgcac
     8041 gccaggcaga accgtcacac ggcggcggtt acacaacgta aaggttgaga aacctgacgg
     8101 cctgcggcaa aatacagtcg tgccggggca cgtccgccag cgatcagcgt gccgccggcc
     8161 tgcatgaggg ggaaacattc agatggcgac attcaccgaa gaccagatca tcgaggcgat
     8221 tcgccccgtc caggatcccg accttggccg ctcactggtc gagctcaaca tggtcaaggg
     8281 agttgagatc gacggcggcc ggatcaagat caacgtcgaa ctgacgacgc cggcctgccc
     8341 gctgcgcggc aagatcgagg acgacgttcg cgccgccgcc gccgccgtcc cgggtgtcga
     8401 ggatgttgcg gtcgccttca cgtcccgcgt ccggcccagc ggcaagggca tgcccgacaa
     8461 gaccccactg accggcgtca agaacgtgat cgccgtcgcc tccggcaagg gcggcgtcgg
     8521 caagtcgacg gttgccgtca acctggccgc ctcactggcc cgcgacggcg ccagcgtcgg
     8581 cctgctggat gccgatgtct acggccccag catcccgctg atgatgggca ccagcgccaa
     8641 gccgatcatg cagaacaaca agatcgtccc gctggaagcc ttcggagtca agattatgtc
     8701 gatcggcttc ctgctggagg ccgaccgggc gctgatctgg cgcgggccgc tggtggcaca
     8761 gctcatcaac cagttcctca acgaggtcgc ctggggcgag ctcgactacc tcgtcatcga
     8821 cctgccgccc ggcaccggcg atgtccagtt gacgctggtt cagaagatcc cgctctccgg
     8881 cgcggtgatc gtcacgacgc cccaggatgt cgcgctggcc gacgcgatca agggcatcca
     8941 gatgttccag gaggtgaaga cgccgatcct cggcatcatc gagaacatgt cgttcttcat
     9001 ctgcccgtcg tgtggtgagc agaccgagat cttcggcttc ggtggcggcg agcggacagc
     9061 cgagcgctac aacgtcccgc tgctgggacg gatcccgctc gacacggccg ttcgccaggg
     9121 cggcgacatc ggccacccga tcaccgtttc tgaaccggaa tcgcctcccg gcgaagcctt
     9181 ccgcgacgcg gcccgccagt gcgcccgcca gctcgcgatc gacgccgtcg agaagccccg
     9241 ccgcgc
//
LOCUS       DASUGJ010000790         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_407217, whole genome shotgun sequence.
ACCESSION   DASUGJ010000790 DASUGJ010000000
VERSION     DASUGJ010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407217"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFV93_17865"
     CDS             <1..314
                     /locus_tag="VFV93_17865"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5167279.1"
                     /translation="TMALEWAHYGIRVNCIAPGYIKVAESEAEQERYRHEEQHATPRI
                     PLRRTGRPSEIASAVAFLVSDDASYITGRTLFVDGGLLVPPLTTSDYLAGDREGRGFS
                     G"
     gene            547..1692
                     /locus_tag="VFV93_17870"
     CDS             547..1692
                     /locus_tag="VFV93_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167280.1"
                     /translation="MQPVRSADYPAHPAMSALLALPSETPRRDADGEQALAAWSAYLG
                     ADLPLLLVRNPDRARKLLMRAFGIAGKEPVGIPVNCRRPLSEAVKRSGGKPLFVELDA
                     DLNLDPASPGLDGVRLIWAEPVAGMSPPPPLPDAELFVDYGFTLPAPLADGAAGLTGA
                     ATLWGLHLAGGEAGALIAFADAALYEASLAAMTDDDSFSDDSLAIAQAERLSGPDGLA
                     ARLLAVDAATREGMEAGAGMPMAPLAGRCALPFGLTVRVPEEADLATFISYIRNENVD
                     LDWLPEIQPMFYVAYQVTTDRARTLQSAANLARWVVAPLGPDFVDDEITHAVLGILKA
                     AEYTGVRWYTQPERAQWYGDLMFEWYGPDHDAYRVQFETGALVAAGD"
     gene            complement(1768..>2052)
                     /locus_tag="VFV93_17875"
     CDS             complement(1768..>2052)
                     /locus_tag="VFV93_17875"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEX5167281.1"
                     /translation="LGDFDLGVGEETATTSPAPGEILFYPAGISETEILFPYGQASFA
                     SKFGPLAGNHFLTVVDGAERLEALGELVVWHGAQEIVFDVDRESGSGIRD"
BASE COUNT          317 a          710 c          663 g          362 t
ORIGIN      
        1 tgacgatggc gctcgaatgg gcgcactatg gcattcgcgt caactgcatc gcgcctggct
       61 acatcaaggt cgcggagtcc gaggccgagc aggagcgcta ccgccacgag gagcagcacg
      121 ccacgccgcg catcccgctg cgccgcaccg gccgcccgtc cgagatcgcc agtgccgtcg
      181 ccttcctcgt ctccgacgac gcctcctaca tcaccggccg caccctcttc gtcgacggcg
      241 gcctgctcgt cccgcccctc accacctccg actacctggc cggcgaccgc gaaggccgcg
      301 ggttttcggg gtgataggga tgtcgtgacg cgtgatgcgt gatgcggtag tgttttcggc
      361 cttcgtgtca cgtttcacgc gtcacgcgtc acgacatccc ttgacacctc tccaccctca
      421 ctgagataat cccgactatt atcatcggga ttacccgcag cggggaggga ctgtcgcgtg
      481 ccgttcgatc gtgatacctg ggccaaccag tggctgggcg gcttctgaac gctgggtgtt
      541 gaagaaatgc agccggtccg gtctgcagac taccccgccc atccagccat gagcgccctg
      601 ctggcgctgc cgtccgagac accacgtcgt gacgccgatg gcgagcaggc gctggccgcc
      661 tggtctgcct acctcggcgc ggatctcccg ctgctgctgg ttcgcaaccc ggaccgcgcc
      721 cggaagctgc tgatgcgcgc gttcgggatc gccggcaagg agccggtcgg cattcccgtc
      781 aactgccgac gaccgctttc ggaagccgtc aagcgctctg gtggcaagcc gctgttcgtc
      841 gagcttgatg cggatcttaa cctcgatccg gccagtcccg gcctggatgg tgtccgtctg
      901 atctgggccg agccggttgc ggggatgtcg ccaccgccgc cgttgcccga cgcggaactg
      961 ttcgttgact acggcttcac gctgccggct ccgctggcgg acggcgccgc gggcctgacc
     1021 ggcgccgcga cgttgtgggg cttgcatctc gctggtggtg aagccggcgc gctgatcgcc
     1081 ttcgccgatg ccgcgctcta tgaggcgtcg ctggcggcga tgaccgacga cgacagtttc
     1141 agcgacgact cactggcgat cgcacaggct gagcggctgt ccgggcccga cggtctcgcc
     1201 gcccggctgc tggccgtcga tgccgccacc cgcgagggga tggaggccgg cgccgggatg
     1261 ccgatggcgc ctctggccgg ccgctgtgcg ctgccgttcg ggctgacagt tcgcgtgccg
     1321 gaggaagccg atctggcgac gttcatctcc tacatccgca acgagaacgt cgacctggac
     1381 tggctgccgg agattcagcc gatgttctac gtcgcctacc aggtaacgac agatcgcgcg
     1441 cgaaccttgc agagcgccgc caatctcgcc cgctgggtcg tcgccccgct cggcccggac
     1501 ttcgtcgacg acgagatcac ccacgccgtg ctcggcattc tcaaggccgc cgagtacacc
     1561 ggcgtccgct ggtacaccca gccggaacgt gcccagtggt acggcgacct gatgttcgag
     1621 tggtacggcc cggaccacga cgcctaccgc gtccagttcg agacgggcgc gctggtcgcg
     1681 gctggggatt aactcgggac acgggaaccg ggatcacgga ttcgggactg gtgtggcaaa
     1741 ccggttcagc tggatatacg aattggctca gtcccgaatc ccggatcccg attcccgatc
     1801 cacgtcaaac acaatctcct gcgccccgtg ccagacgacc agttcaccga gtgcttcgag
     1861 tcgctcggcg ccgtcaacaa ccgtcaggaa gtggttaccg gccagcgggc cgaacttgct
     1921 ggcgaatgat gcctgcccgt atgggaacag aatctccgtc tcggagatcc cggccgggta
     1981 gaagaggatc tctcccggtg cgggcgacgt cgtcgcggtc tcctcgccga cgccgagatc
     2041 gaagtcgccc ag
//
LOCUS       DASUGJ010000791         4929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1240639, whole genome shotgun sequence.
ACCESSION   DASUGJ010000791 DASUGJ010000000
VERSION     DASUGJ010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240639"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFV93_17880"
     CDS             <1..197
                     /locus_tag="VFV93_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167282.1"
                     /translation="VSDDLAPGRPVRLVGGRSLGTVGTCQSPAYQRVTDAGVVTTVVD
                     VALPGGDARVVAAANIEVLH"
     gene            complement(424..2295)
                     /gene="dnaK"
                     /locus_tag="VFV93_17885"
     CDS             complement(424..2295)
                     /gene="dnaK"
                     /locus_tag="VFV93_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX5167283.1"
                     /translation="MARTIGIDLGTTNSVVAVIEGGEPTVITNTEGERLTPSVVALSS
                     SNERLVGRFAKRQAVTNAKNTIFSVKRFIGRRFDDPVVQRDIKLVPYEVRQGAHGGID
                     IKMGDKWSTPAEISAMVLQKLKADAEAYLGDKVDKAVITVPAYFDDSQRQATKDAGQI
                     AGLEVLRIINEPTASALAYGLDKKTDEKVAVYDLGGGTYDISILDLSEGVFQVLATNG
                     DTHLGGDDFDQRVIDFLADEFKKTEGIDLRSDRMALQRLKEAAEKAKIELSTTQQTEV
                     NLPFITADASGPKHMNITLTRSKLEQLVDDLIKRSIGPMEAALRDAGLSKDQLDEILL
                     VGGQTRMPAVQKAVTDFFGKEPHKGINPDEVVAIGAAIQAGVLGGEVKDILLLDVTPL
                     TLAIETLGGVATALIERNTTIPTRKSQVFTTASDNQNQVEIHVLQGERPMANDNKTLA
                     RFILDGLPPAPRGIPKIEVTFDIDANGILNVSAKDQATGREQKVTITASSGLSQDEID
                     KMMKEAEAHAAEDKQRREAIEFRNQAEAMTYQAERTLSDYGDKIPSETKLDLETKVAT
                     VRDILANDPENIDRLRTAYDDMTQTLSQAGASMYEQAGAPEDMGPDGATAEEQPAED"
     gene            complement(2346..2906)
                     /locus_tag="VFV93_17890"
     CDS             complement(2346..2906)
                     /locus_tag="VFV93_17890"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5167284.1"
                     /translation="MDKTLTDNHPNAATDGQPSMEEVLAELEQFKADAADMLDRLQRS
                     QAEFANFRRRNEQEREIQRARATEDIIRKLLPVADDFDRALRSVPAEAEGNPWIEGIR
                     LVERKLWKVLEGEGISLMESVGQPFDPSRHEAIMVDEGAGAADTVIEEFQRGYFVHDT
                     VLRPAMVKVGSAESLSATAGVADSKH"
     gene            complement(2975..4009)
                     /gene="hrcA"
                     /locus_tag="VFV93_17895"
     CDS             complement(2975..4009)
                     /gene="hrcA"
                     /locus_tag="VFV93_17895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX5167285.1"
                     /translation="MSDDNELSVRQREILRLIIQEYVIGGRAIGSQSLVERYPLNISS
                     ATVRNEMSALERLGFIEQMHTSAGRVPTDRGYRFYVEQFASDSSLPANDQIMIRHQFR
                     QVETQLESWLELAASVLAGFARNVSVVTSPRTTVSRLRHFELLSLQERIVLLIMVTQE
                     STVHQAMLHVPEPVTQEDLSACADRLNAVFQNLTVDELRSRVIGLADLDKQVGEHLLA
                     TLESTQASERTEVHYQGIEHVLHQPEFGGSPLAQQLLEMLRGGSLLAELLPQISDRDG
                     LQIFIGEENASEELRPFGVVVATYGVDEEVTGLLGILGPRRMPYERSISSVRYMARLM
                     SDLMRDLYSA"
     gene            complement(4303..>4929)
                     /locus_tag="VFV93_17900"
     CDS             complement(4303..>4929)
                     /locus_tag="VFV93_17900"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HEX5167286.1"
                     /translation="PGGGAAREIRRMIPHEVSHLILHQATENPWNSPPNWLDEGLAVY
                     NQETPDADLPGILDDAVDDGELIPVSALGASFPLDPDQAYLSYAESLSIVTYIVEELG
                     ESEMAELIAVFRDEVSYDEAVERSLGMSTEELDTAWKDWLGYQGDSQPASDAGEPPRP
                     QIDTDEGDDLSHNEWIGFLACTGIFALGGLALGIVSIRKVRAIGKTRT"
BASE COUNT          764 a         1627 c         1555 g          983 t
ORIGIN      
        1 gcgtctccga cgacctggcc ccgggccgtc ccgtccgcct cgtcggcggg cgctcgctcg
       61 gcacggtcgg cacctgccag tcccccgcct accagcgtgt cactgacgcc ggcgtcgtca
      121 caaccgtcgt tgatgtcgcg ctccccggcg gcgacgcccg cgtcgtcgcg gcggcgaata
      181 tcgaggtgtt gcactaggaa cgattcacaa ttcaatgccg atccggtagg ggcgatatcg
      241 caatatcgcc ctttttgacg cccggcgcat ggagcagggc gattttgcga tatcgcccct
      301 accggattgg cattgaattg tcatggatac agaacgaccg ccccccatcg gagggcggtc
      361 gtttcgtttg cgttgtctga tttagttgtc gccgacctca cggaacacgc cttcgacggt
      421 cgtttagtct tcggcgggct gttcctcagc ggtggcgccg tcggggccca tgtcctcggg
      481 cgcgccggcc tgctcgtaca tcgacgcgcc ggcctgcgag agcgtctgtg tcatgtcatc
      541 gtaggccgtc cgcaaccggt cgatgttctc cgggtcgttg gcgaggatat cgcggacggt
      601 cgcgaccttc gtttcgaggt cgagcttcgt ctcgctcggg atcttgtcgc catagtcgga
      661 gagcgtccgc tcggcctgat aggtcatcgc ctcggcctga ttgcggaact cgatcgcctc
      721 gcgccgctgc ttgtcctcgg cggcgtgggc ctcggcctcc ttcatcatct tgtcgatctc
      781 gtcctggctc aggccgctcg atgcggtgat cgtcaccttc tgctcccggc cggtcgcctg
      841 gtccttggcc gagacgttca ggatgccgtt ggcgtcgatg tcgaacgtca cctcgatctt
      901 cggaataccg cgcggggccg gcgggagacc gtcgaggatg aagcgcgcca gcgtcttgtt
      961 gtcgttcgcc atcggccgct cgccctgcag gacgtggatc tcgacctggt tctggttgtc
     1021 cgacgcggtc gtgaagacct ggctcttgcg ggtcgggatc gtcgtgttgc gctcgatcag
     1081 cgccgtcgcg acgccaccca gcgtctcgat cgccagcgtc aatggagtga cgtccagcag
     1141 caggatgtcc ttgacctcgc cgcccagtac accggcctgg atcgcggcgc cgatcgcgac
     1201 gacttcgtcg gggttgatgc ccttgtgcgg ctccttgccg aagaagtcgg tgaccgcctt
     1261 ctggacggcc ggcatccggg tctgaccgcc gaccagcagg atctcgtcga gctggtcctt
     1321 tgacagaccc gcgtcacgca gggccgcctc catcgggcca atcgagcgct tgatcaggtc
     1381 atcaaccagc tgctccagct tcgaacgggt cagcgtgatg ttcatgtgct tcggaccact
     1441 ggcgtcggcc gtgatgaacg gcagattgac ctcggtctgc tgcgtcgtcg agagctcgat
     1501 cttcgccttc tcggccgctt ccttgagccg ctggagggcc atccggtccg agcgcaggtc
     1561 gatcccttcg gtcttcttga actcgtcggc caggaagtcg atcacgcgct ggtcgaagtc
     1621 gtcaccaccc aggtgggtgt cgccgttcgt cgccagcacc tggaagacgc cctcggagag
     1681 gtcgaggata gagatgtcgt aggtaccgcc accgaggtcg tagacggcga ccttctcgtc
     1741 ggtcttcttg tcgagaccgt aggcgagcgc cgaggccgtc ggctcgttga tgatgcgcag
     1801 cacttcgagg ccggcgatct ggccggcgtc cttggttgcc tggcgctggc tgtcgtcgaa
     1861 gtaggccggg acggtgatga cggccttgtc gaccttgtcg cccagatacg cctcggcgtc
     1921 cgccttcagc ttctggagca ccatcgccga gatctcggcc ggggtcgacc acttgtcgcc
     1981 catcttgatg tcgatcccgc cgtgcgcgcc ctggcgcacc tcgtacggga cgagcttgat
     2041 gtcgcgctgg acgaccgggt cgtcgaagcg acgtccgatg aagcgcttga ccgagaagat
     2101 cgtgttcttc gcgttcgtga cggcctgccg cttggcaaag cggcccacca gccgttcgtt
     2161 gctggacgag agcgcgacga ccgacggcgt cagccgctcg ccctccgtgt tggtgatgac
     2221 ggtcggctcg ccgccctcaa taacggcgac gactgagttc gtcgtcccca ggtcgatacc
     2281 aattgttcgt gccatacctg tgttcctcct gcccatcccc gcttgcagcc gggggacggt
     2341 cacatctagt gcttcgagtc cgccacaccg gctgtcgcgc tgagagactc ggccgatcca
     2401 accttgacca tcgccgggcg caggacggtg tcgtgtacga agtagccacg ctgaaattcc
     2461 tcgatgaccg tgtcggccgc gcccgcgcct tcatcgacca tgatcgcttc gtgccggctg
     2521 gggtcgaacg gctgcccgac gctctccatc agcgagatgc cctcgccttc gagcaccttc
     2581 cacagcttgc gctccaccag ccgaatgcct tcgatccacg gattgccttc ggcctcggct
     2641 ggaaccgagc gcagggcgcg gtcgaagtcg tctgccaccg gcagcagctt acgaatgatg
     2701 tcctcagtcg cgcgtgcgcg ctggatctct cgctcctgct cgttgcggcg tcggaagttg
     2761 gcgaattccg cctgcgagcg ctggagccgg tcgagcatgt cggccgcgtc ggccttgaac
     2821 tgctccagct cggccagcac ctcttccatc gacggctggc cgtcggtcgc ggcgttgggg
     2881 tgattgtctg tcagcgtctt gtccatgtca tccatttcct gcgtcacaag tggtggtccc
     2941 tccattcgtt cccgctccaa acgctcgctg catgtcaagc gctgtacagg tcgcgcatca
     3001 ggtcgctcat cagccgtgcc atgtagcgca ccgatgaaat cgagcgttcg tacggcatcc
     3061 ggcgcgggcc gagaatgccg agcaggccgg tgacctcttc gtcaacgcca tacgtcgcaa
     3121 caacgacgcc aaacggccgc aactcctcgg aggcgttttc ctcaccgatg aagatctgca
     3181 acccgtcgcg gtcggagatc tgcggcaaca gctccgccag cagtgatccg ccgcgcaaca
     3241 tctccagcag ttgctgggcc agcggcgatc cgccgaattc cggctgatgc aggacgtgct
     3301 cgattccctg gtaatgcacc tcggtccgct cgctggcctg ggtcgattcc agcgtcgcca
     3361 gcagatgctc gccgacctgc ttgtcgaggt cggccagccc gatcacccgc gagcgcaact
     3421 cgtcgaccgt cagattctgg aagacggcgt tgagccggtc ggcgcaggcg ctcagatcct
     3481 cctgggtcac tggttcaggc acgtgcagca tcgcctggtg gacggtgctc tcctgggtga
     3541 ccatgatcag caggacgatc cgctcctgga gggacagcag ctcgaagtgg cgcaggcgcg
     3601 agacggtagt ccggggcgat gtcacgaccg agacgttgcg cgcgaagccg gccagcacgc
     3661 tggcggccag ctccagccac gactcgagct gcgtctcaac ctgccggaac tggtgccgga
     3721 tcatgatctg gtcgttggcc ggcagcgacg agtcggacgc gaactgctcg acatagaagc
     3781 ggtagccccg gtcggtcggc acccggccgg ccgacgtgtg catctgctcg atgaagccga
     3841 gccgctccag cgccgacatc tcattgcgaa ccgtcgcaga cgagatgttg agcgggtacc
     3901 gctcgaccag cgactgcgag ccaatcgccc gcccgccgat gacatattcc tggataatca
     3961 ggcgcaggat ctcccgctgc ctgactgaca gttcgttgtc gtcactcatg ttcatccgcc
     4021 ttttagcact cttttcacct gagtgctaac cacagaatat catagcaggt tgatacgggt
     4081 agtgtcaagg ttgcaggcga gtacgtacag gcgcgaccat caacaagacc gccgaatcgc
     4141 acaatcaatc gcgtaggggc gatatcgcaa tatcgcatgg ctctcccgcc cgaatgcctc
     4201 ggtggggaag caaggcgata tcgcccctac gcgattgatt gcgggatgag gaatgcggca
     4261 tccgcgatcg ccccgagtcc cgactcccga ctcccgaatc ctctacgtcc gcgtcttccc
     4321 aatcgcccgg accttgcgga tcgagacgat gccgagcgcc aggccgccga gcgcgaagat
     4381 gccggtgcag gcgaggaagc caatccactc gttgtgggag agatcatcgc cctcgtcggt
     4441 gtcgatctgg ggccggggtg gctcgccggc gtcgctggcg ggctggctgt cgccctgata
     4501 gccgagccag tctttccaag cggtgtccag ctcctcggtg ctcatcccga gcgatcgttc
     4561 aaccgcttcg tcgtaggaga cctcatcgcg aaagacggcg atcagctccg ccatctccga
     4621 ttcgcccagt tcctcgacga tgtaggtcac gatcgagagg ctctctgcgt aggagaggta
     4681 ggcctgatcc ggatcaagcg ggaagcttgc gccgagcgcg ctgaccggga tcagctcgcc
     4741 gtcgtcgacg gcgtcgtcaa ggatgcccgg cagatcggcg tctggcgtct cctggttgta
     4801 gacggccagt ccctcgtcaa gccagttggg gggcgaattc cacgggttct cggtcgcctg
     4861 atgcaggatc aggtggctga cctcgtgcgg gatcatccgg cggatctcgc gcgccgcgcc
     4921 gccgcccgg
//
LOCUS       DASUGJ010000792         6583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1102566, whole genome shotgun sequence.
ACCESSION   DASUGJ010000792 DASUGJ010000000
VERSION     DASUGJ010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102566"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..641
                     /locus_tag="VFV93_17905"
     CDS             297..641
                     /locus_tag="VFV93_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167287.1"
                     /translation="MAIGKLTRTTVLLLLLILTAAQTGCISDDQDSDNRDQAINEAQR
                     AYAAKLAEGTDFSSGPCIAEEVIDDWSVDIAHDPRQAIDDDPANQCTAYRNGTTHHFV
                     ELDPEGNLIRAK"
     gene            complement(732..1625)
                     /locus_tag="VFV93_17910"
     CDS             complement(732..1625)
                     /locus_tag="VFV93_17910"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5167288.1"
                     /translation="MRLDEIQRVTVVGAGLMGHGIAQEFAVAGYEVRLHDTSDANLER
                     AMAGIRANLALMANAGMVDQEQANAAPDRLHPTTSLAEAGSDADVVVEAVFENLELKQ
                     QIFAELDEVCPPHAILASNSSSYMPGKLAASTARPAQVLVAHYFNPPHLMPLVELVRH
                     PETSDETVETMFALYMRIGKKPVIIQHEAPGFVANRLQMAIWREAANIVARGICAPQD
                     VDTVVRYGFGRRYAAGGPFELGALISADVRRAVAAEIMPDLANSVPDLDRGAYSLTAE
                     EAEQVRLRVAHALLEIAKWDG"
     gene            1983..2612
                     /locus_tag="VFV93_17915"
     CDS             1983..2612
                     /locus_tag="VFV93_17915"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5167289.1"
                     /translation="MDIYEALYTTRSMRKVTQEPIPHDVQERIMDAAIRAPSGSNRQP
                     WRFLLVDDADLRTQLAALYLDGWQQLNLSRYGTTTPDFDSVRRSADYLARHFAEVPLL
                     LFAFGQGSSESSVYPALWSAMLAARAEGVGTTLTTVLSYHRDEVFELLGVPADEDWRM
                     YGCVTMGYPTGRWGVAARRPLAEVTARNHWNTAAGFTTGEPLWSAADKR"
     gene            complement(2621..4795)
                     /locus_tag="VFV93_17920"
     CDS             complement(2621..4795)
                     /locus_tag="VFV93_17920"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEX5167290.1"
                     /translation="MDEAKSRWLGSGTISPDEPVEAGASGTWTIVYTAGVAGIDDGGR
                     IRIAWRSVSDWPAPQFSAPEQANYATVSTDAPVTLTPVYGSDGVRPWNRTLTIRVSDG
                     ALAAGETVTVVLGDTSGGSPGMRAQTFPERRFRFKLQADPFATGVYEDVTDLGFPIVG
                     GEAVRFEIVVPSDVVAGEPFRLQVRALDRWGNPDPAYRGTCRFIGEKPGGLPDSYTMT
                     AQDSGIHWFERLTLEQPGVSSIAVEDGDGRTGRSNPLRCHAEQPEWRLYWGDPHGQTE
                     ETVGTGSIDDYLAYARDVAAINMTGHQGNDFQITAEVYERIQSALAGANEPGRFVTFF
                     GYEWSGNTPAGGDHNVHYRDGGPIHRSSHTLIPYRSDIDSDRYPVDALYATFAGREDV
                     LLVPHIGGRHASLAYHDPALEPVIEIASQWGRFEWFARDALERGLKVGFVGGSDDHSG
                     RPGWSAATVAHHGVRGGLMAILAPDLTRESLWDALRARRCYGTSGPRIILDLSVSGHP
                     MGSEPTLTEPPLVRCHVIGATDLDTIEIRRGLETVHVEHLLPQPRPGDRQRIRLAWRG
                     ARNRGRGRALDWSGRLDLRGGRITGAENYAIDHPLDGITGWDATHVAWRSHTVGDWDG
                     IILDLEGDDSSVIDVSTPTMSLCLALDDIPRDGYRQTGDLLEQQLVVRRIGTGPGRRE
                     AQIEWQDTTPTPGVNPYWIWVTQADGELAWSSPVYATVGKAQ"
     gene            complement(4857..5516)
                     /locus_tag="VFV93_17925"
     CDS             complement(4857..5516)
                     /locus_tag="VFV93_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167291.1"
                     /translation="MRRMFVVWVALVSVAGFVAVARRRGATRPDRSIRFDPVVVGTIE
                     ADGWRAYYDRDFVTGFRLLLRLMREQFGLGPLGAIRAAHAAVRAQRAFAPVANDLDAT
                     LRWLTRFYELSPRRDGVAAVDLARAELDYWIVHRRIVDQADKRELVDAFTRLHVLLFG
                     GDEVTMRPSAEQRTLACNTVDRITGKRSLDLEGDWRSAREHLIAAYQLAVAASPVQAS
                     N"
     gene            5910..>6583
                     /locus_tag="VFV93_17930"
     CDS             5910..>6583
                     /locus_tag="VFV93_17930"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5167292.1"
                     /translation="MAEAGQQRWQLALTLVAFAIPLVVALVQPDVGGLSPWGVRVLAV
                     VAASLLLWLSEVLPIGVTSLAVVAMLALVAPGESSEGLRLSLAGFESPAPYFMLGALT
                     LGTATVRTGLAQRFARFLVRGAKGSGDRLYGQMIAMMPLMAILVPSALTRSAMLIPAY
                     EAIFESHRIERGHRLPRLIMLATATVQIQASTAVLTGGAVPVVAGALLGDLSWARWFM
                     YMAVPN"
BASE COUNT         1052 a         2241 c         2180 g         1110 t
ORIGIN      
        1 agcgcggcgg agcgatcgat cccccggatg gtcgcgctgg cctggctgga gaggacgaaa
       61 caggcgtcca gcatgcccgg caggcggatg tcggcggcgt agcgttcacg gccggtcagc
      121 ttcgggccgg aatcgggccg cttcatgcgc cggccaagca ctcggaactg ctcgctcatc
      181 tccggctctc accctcccct ggcacgttga cgctcgcggc tgccaccgtc agaatcgcgg
      241 cgttcactat accgtgcctt gtcagccacg gcgccttggc tggatcggag tcgaagatgg
      301 cgatcgggaa gctcacgcgg acgacggtgc tgttgctgct gctcatcctg actgccgccc
      361 agaccggctg catcagcgat gaccaggaca gcgacaaccg cgaccaggcc atcaacgaag
      421 cccagcgcgc ctacgcggcc aagctggccg aaggaaccga cttttcaagc ggcccctgca
      481 tcgccgaaga agtcatcgac gactggtccg tggacatcgc ccacgacccc cgtcaggcca
      541 tcgacgacga ccccgctaac caatgcaccg cctaccgcaa cggcaccacc caccacttcg
      601 tcgaactcga tccagaggga aacctgattc gggcgaagtg aaggcgtcgg gattcgggaa
      661 ccgggattgg gggcgctgat acggccccgc ttcaccccca atcccggttc ccgaatcccg
      721 aatcccgacc gtcatccatc ccacttcgcg atctcaagca gcgcgtgggc gacccgcagg
      781 cgaacctgtt cggcctcttc tgcggtgagg ctgtaggctc cgcggtcgag gtcggggacg
      841 ctgttggcca ggtcgggcat gatctcggcg gcgacggcgc ggcgaacatc ggcgctgatc
      901 agcgcaccca gttcgaacgg cccaccggcg gcgtagcggc ggccgaagcc gtagcggacg
      961 accgtgtcaa cgtcctgcgg cgcgcagatg ccgcgcgcga cgatgttggc cgcctcacgc
     1021 cagatcgcca tctggagccg gttcgcgaca aagcccggcg cttcgtgctg gatgatgacc
     1081 ggcttcttcc caatccgcat gtagagcgcg aacatcgtct cgaccgtctc atcggatgtc
     1141 tccggatggc gcaccagctc gaccagcggc atcaggtggg gcgggttgaa gtagtgggcg
     1201 accaacacct gggccggtcg agccgtgctc gccgccagct tgcccggcat gtacgacgac
     1261 gagttgctgg cgaggatcgc gtgcggcggg cagacctcat ccagctcggc gaaaatctgc
     1321 tgcttcaatt ccaggttctc gaagaccgct tcgaccacga catcggcatc cgacccggcc
     1381 tcggcaagcg acgtcgtcgg atgcagccgg tccggcgcgg cgttggcttg ctcctgatcg
     1441 accatcccag cgttcgccat cagcgccagg ttggcgcgga tgccggccat cgcccgctcc
     1501 agattcgcgt cgctcgtgtc atgcagccgt acctcgtagc cggcgacggc gaactcctgg
     1561 gcgatgccgt ggcccatcag cccggccccg acgaccgtca cccgctggat ctcatcaagt
     1621 cgcatcccgt ccgctccctt cgcccacatc catttgccgt tgattccaac cccgtggata
     1681 ctacccgcaa caggcggctc ggcaataccc agcagcgatg ctggccgcgt cggacagcaa
     1741 gacgaatagg ggccgaacgc tcgacgagga accatccatc gcccccctgt catcccgacg
     1801 caggagggat ctcgttctcg ctgaaacggg tacggaccga acgagcgacg agatccttcg
     1861 ctgtcgctca ggatgacaga ggggaggggt gcggttcctc gcagagcaat cggccctgga
     1921 ctaccccgaa cccgaatccc ggttcccgaa tcccgaatcc cgcaacagaa aggcacctac
     1981 gcatggacat ctacgaagcg ctctacacga cacgctcgat gcggaaggta acgcaggagc
     2041 cgattccgca cgatgtccag gagcgaatca tggacgcggc gatccgtgcg ccgagcggca
     2101 gcaaccggca gccgtggcgc ttcctgctgg tcgatgatgc cgacctccgg acgcaactcg
     2161 ccgcgctcta tctggatggc tggcaacagc ttaacctgtc gcgctacggg acgacgacgc
     2221 cggatttcga cagtgtccga cgctcggctg actacctcgc ccgccacttc gcggaggtgc
     2281 cgctgctgct cttcgccttc gggcagggat cgtctgagag ttccgtctac ccggcgctct
     2341 ggagtgcgat gctggccgcc cgcgccgagg gcgtcggcac gacgctgacg acggtcctca
     2401 gctaccaccg cgacgaggtc ttcgagctgc tcggcgttcc tgccgacgag gactggcgca
     2461 tgtacggttg cgtcacgatg ggctacccga ccggccgctg gggcgtcgcc gcccgccggc
     2521 cactggccga ggtaacggcc cgtaaccatt ggaacaccgc tgccggattc acgaccggcg
     2581 agccgctctg gagcgcagcc gacaagcgct gatcgggctg ctactgcgct ttcccgacgg
     2641 tggcgtagac cgggctgctc caggccagct cgccgtcggc ctgggtgacc cagatccagt
     2701 acgggttgac acccggcgtc ggcgtggtgt cctgccactc gatctgcgct tcacggcgac
     2761 ccggcccggt gccgatccgg cggacgacga gctgttgttc gaggagatcg ccggtctgcc
     2821 ggtagccgtc acgcgggatg tcgtccagcg caaggcagag cgacatcgtc ggcgtgctga
     2881 cgtcaatgac gctgctgtcg tcgccttcga gatcgaggat gatgccgtcc cagtcgccga
     2941 cggtatgact gcgccatgcg acgtgggtcg cgtcccagcc ggtgatcccg tccagcggat
     3001 gatcgatggc gtagttttcc gcgccggtaa tccggccgcc gcgcaggtcg agccgcccgc
     3061 tccagtcgag cgcccggccc cggccccgat tgcgtgcgcc gcgccaggcg aggcgaattc
     3121 gctgacgatc tcccggcctg ggttgcggta gcaggtgctc gacatggacc gtctccagcc
     3181 cgcgccggat ctcgatcgtg tcgaggtcgg tcgcaccgat cacgtggcaa cggaccagtg
     3241 gcggttccgt caaggtcggc tccgatccca tcgggtgccc gctgacggac aggtcgagga
     3301 tgatgcgcgg gccgctcgtt ccgtagcagc gacgggcgcg gagtgcgtcc cagaggctct
     3361 cgcgggtgag atcgggcgcg aggatcgcca tcagcccacc ccggacgccg tggtgggcga
     3421 cagttgccgc gctccagccg ggccggccgc tgtggtcgtc cgagccgccg acgaagccga
     3481 ccttcagtcc ccgttcgagg gcgtcgcgcg cgaaccactc gaagcgcccc cattgcgagg
     3541 cgatctcgat caccggctcc agggcggggt cgtggtaggc gaggctggca tgccgcccgc
     3601 cgatgtgcgg cacgagcagc acgtcttcgc ggccggcgaa ggtcgcgtag agcgcatcga
     3661 ccgggtagcg gtcgctgtcg atatccgagc gatacgggat caacgtgtgg ctcgagcggt
     3721 ggatcggccc gccgtcgcgg tagtggacgt tgtggtcgcc gccggccggc gtgttgccgg
     3781 accactcgta gccgaagaag gtgacgaagc ggcccggttc gttggcgccg gccagtgctg
     3841 actgaatccg ctcgtagacc tcggccgtga tctggaagtc gttcccctga tgaccggtca
     3901 tgttgatggc ggccacatcg cgtgcgtagg cgaggtaatc gtcgatcgag ccggtaccga
     3961 ccgtctcctc ggtctgcccg tgcggatcgc cccagtagag ccgccactcc ggttgctcgg
     4021 catggcagcg cagcgggttg ctccgtccgg ttcgcccgtc gccgtcctca acggcgatgg
     4081 aggagacgcc tggctgctcg agcgtcagtc gctcgaacca gtggatgccg ctgtcctgtg
     4141 ccgtcatcgt gtatgaatcc ggcagcccgc cgggcttttc accgatgaag cggcacgtgc
     4201 ctcgataggc gggatcagga ttgccccagc gatcgagcgc gcggacctgc aaccggaacg
     4261 gctcgccggc cactacgtcg ctcgggacga cgatctcgaa ccggaccgcc tcgccgccga
     4321 cgatcgggaa acccaggtcg gtgacgtcct cgtacactcc cgttgcgaac ggatcggcct
     4381 gcagtttgaa gcggaaccgt cgttcgggaa acgtttgcgc gcgcatgccc gggctgccgc
     4441 cggacgtgtc gcccagcacg accgtgacgg tttcgccggc cgccagcgcc ccatcggaga
     4501 cgcggatggt cagcgtgcgg ttccagggcc gaacgccgtc cgagccatag acgggtgtga
     4561 gcgtcaccgg cgcatcagtg ctgacggtcg cgtagttggc ctgctctggc gcgctgaact
     4621 gcggtgccgg ccagtcggag acgctccgcc aggcgattcg aatccgcccg ccatcgtcga
     4681 tcccggccac gccggccgtg tagacgatcg tccacgttcc tgacgctccg gcctcgaccg
     4741 gctcatcggg tgagatcgtc ccgctcccca gccagcggct cttcgcctcg tccatccgcc
     4801 cagcccttcc gctacgctgc tgtgtcttgg ggcagtgtaa ccggatggcc ctggcgtcag
     4861 ttcgaagctt ggacaggtga cgccgcgacg gcgagctggt aggcggcgat cagatgctcg
     4921 cgggcgctcc gccagtcacc ttcgaggtcg agcgatcgct tgccggtgat ccggtcgacc
     4981 gtgttgcagg ccagcgtgcg ctgctcggcc gaggggcgca tggtgacctc gtccccgccg
     5041 aacagcaaca catgcagccg ggtaaacgca tcgacgagct cacgcttgtc agcctgatcg
     5101 acgatccgac ggtggacgat ccagtaatcc agttcagcac gagcaagatc gaccgccgcg
     5161 acaccgtctc ggcgcggcga gagttcgtag aaccgggtca gccagcgcag cgtcgcgtcc
     5221 aggtcattgg caaccggcgc gaaggctcgc tgagcgcgga cggctgcgtg tgccgcccga
     5281 atggcgccga gtggaccaag cccgaactgc tcgcgcatca gccgcagcaa caacctgaag
     5341 ccggtgacga agtcccgatc gtagtacgct cgccagccgt ccgcttcgat cgtcccaacg
     5401 acaactgggt cgaaccgtat gcttctatcc gggcgcgtag ccccgcgacg gcgtgcgacg
     5461 gccacgaatc ccgcgacgct gacgagagct acccagacca cgaacatccg gcgcacggca
     5521 acctccagtc acgtatatcc agtcaggcat ggatacgcac gtaatcgcgg cgtggatcac
     5581 gccgcagttg attctggtcc ttttctctaa atgtggagaa gcgtggtgat gcgatggttg
     5641 ggcgggggtg catgttcggg agcgagtgac aggcgaggtg gggaagtcgg gcgaatgtgc
     5701 gtcatgcgtt gacgtctccc tcgccatatg gcacgccttc gcccagctct cccacccggc
     5761 ctgtcaccct cgccaccgca ccatcacgtg tcaattctcc cggcaacgag aatgagccac
     5821 agtcgccggc cctttcatcg ctcgtccgac tggccgaatc cggcgcatcc ggctacagta
     5881 gcggcggagg gtggggttgg cgcgcgagga tggctgaggc agggcaacaa cgctggcaac
     5941 tggcgttgac gctggtcgcg ttcgcgatcc cgctggttgt tgcgctggtc cagcccgacg
     6001 tcggcgggtt gagcccttgg ggcgtccgtg tcctggcggt tgtcgccgcc tcgttgctgc
     6061 tctggctctc ggaagtgctg ccgatcggcg tcacgtcgct ggcggtcgtc gcgatgctgg
     6121 cgctggttgc gcccggtgag agtagcgagg gattgcggct ctcgctggcc ggcttcgagt
     6181 cgccggcgcc atatttcatg ctcggcgcgc tgacgctggg gacggcaacg gtccgcaccg
     6241 gcctggcgca gcgcttcgcc cgcttcctcg tccgcggcgc gaaaggcagc ggcgaccgtc
     6301 tctacgggca gatgatcgcg atgatgccgc tgatggcgat cctcgttccg tcggcgctga
     6361 cccgttcggc gatgctgatt ccggcctacg aggcgatctt cgagagccat cgcatcgagc
     6421 gcggccatcg cctgccacga ttgatcatgc tggcgacggc gaccgtccag attcaggcat
     6481 cgacagccgt cctgaccggc ggggccgtcc cggtcgtggc cggggcgctg ctgggcgacc
     6541 tctcctgggc gcgctggttc atgtatatgg cggttcccaa cta
//
LOCUS       DASUGJ010000793         5290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1797670, whole genome shotgun sequence.
ACCESSION   DASUGJ010000793 DASUGJ010000000
VERSION     DASUGJ010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797670"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VFV93_17935"
     CDS             complement(<1..1355)
                     /locus_tag="VFV93_17935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5167293.1"
                     /translation="MVETKNGVTTETIPASPKVTIPLTPAGGNSVTVEKQMLKVVPKV
                     GKVDLMQISQTSVKDFAPDLKRLVDVLFVNPPSPDGEVFIRDIHRVGRRSREKMIWPQ
                     TELAGLAAVVEEAGYTTEIIDCIAEDMDWPTFQAKLEYWRPKFLVTNVTAPTIKNDLT
                     TTYIAKSIGTVTIAIGSHVTPMAFETLADYPSLDMVIRHEPEFTLREIIQKLEAGETL
                     HGVQGCVFRHEGEVVANPDRPFIANLDDLPIPLYHKLPLQKYRIPMIRGPYCFVVTSR
                     GCPAGCRFCIKHVMWQNTVRSHSAERILEEMKVLASLGVTNIHFYADLFTVDRQVVID
                     LCKLIVEDGTKFRLTSNSRVDFVDEEMLTWMAKAGFWMLSWGLESGSIDVLKRMRKGI
                     NFDKTRRALETSHKLGIKNWAYFIIGMPGETVASIDETIAFAKSLPLTLALFHIAVPY
                     PG"
     gene            complement(1418..2845)
                     /locus_tag="VFV93_17940"
     CDS             complement(1418..2845)
                     /locus_tag="VFV93_17940"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5167294.1"
                     /translation="MEQLTHHQRVAVLGGGVAGLSAAWKLAEAGMGVDLYEAAPALGG
                     MAATMRRDGYLWDFGPHRFHSSNPAIIETVKDLVGDDLEGRRRTTRVYFMGDFYDYPL
                     NATNLLSNLPKTLAVTSMLDFMYTRARQRIRPTPDDSFETWVVNRFGRRLYDVYFGPY
                     TAKVWGRDPRQLSASWAAQRVAVVDLWDLLLRVMKLRRGDNGFHHSEFKNDFWYPRNG
                     IGQIAEAMAERATNAGANLLTGARISAVEHDGQAIRAVEYQHNGETIRQECDACVSSL
                     PLPLLVQLLRPAPPEEVLQAARSLQYRAMIFLFLEIDKPKVTDDHWTYFPGPMIFNRI
                     SEMGNFTHDAAPEGKTSVTLEITCDIGDEIWTMPEDEMYTRAVRELAATGLIEERQVL
                     GHFFRRLSHAYPTYDVDFEAKIGHMAYHLADYDNLINAGRQALFRYVNTDHVMEMGFC
                     AAQELLENSLGSRVERVGKEPVWFG"
     gene            complement(2880..3647)
                     /locus_tag="VFV93_17945"
     CDS             complement(2880..3647)
                     /locus_tag="VFV93_17945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5167295.1"
                     /translation="MLAQVQAYIGTQASGVGAYLKQETLTSLLSWIPSLPGIAARGIG
                     YRLMLQADGAPAIEQSVRLRYAENIRLGRGVYLDHSTYLHACPGGITIGDDTLVMHGA
                     ILHVYNFRGLPNAGITIGKRCIVGEQTVIRGQGGVQIGDDVLIAPGVQILAVDHVIET
                     SRVPIMQQGLKTRGITIESGAWLGAGAVVTDGVRVGRNAVVGAGAVVTRDIPDNTVAL
                     GVPARLIRTIEDVEPPADLAPPILHSIRGGAVAARFG"
     gene            complement(3948..4613)
                     /gene="gmhB"
                     /locus_tag="VFV93_17950"
     CDS             complement(3948..4613)
                     /gene="gmhB"
                     /locus_tag="VFV93_17950"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEX5167296.1"
                     /translation="MGRPAVFLDRDGVLNENRDQYVRSWEEFSFLPGTFAALRGLNTL
                     DLPVVVITNQAGVGRGLIPEERIDEIHQRMAAEIARQGARIEQIYWCPHTTQDDCDCR
                     KPKPGLLLRAAEQLGIDLAASVFVGDAETDVEAGKRAGCRTVLVLTGRGRDAVRKMAG
                     RSDRKTVPDAIAADLEAALPYLKQILPSKPAREFWWPDWSQLQSAFSADGLSSPVLQV
                     GKG"
     gene            4905..>5290
                     /locus_tag="VFV93_17955"
     CDS             4905..>5290
                     /locus_tag="VFV93_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167297.1"
                     /translation="MSSTGSVEGGMRGQSGDFAGQRRLSVAVAGLNLDLAVAGVVLLM
                     LLLGFIRLDRYPAPWFDEGIHLQVAKNLVADGTYAARSADGTLDYAPAIGVGPTLLLP
                     VAAAQQLFGTSLEASRTVPVLYLLLAT"
BASE COUNT          944 a         1706 c         1674 g          966 t
ORIGIN      
        1 ccgggatacg ggacggcgat atggaagagt gcgagcgtca gcggcaggct cttggcgaag
       61 gcgatcgtct cgtcgatcga cgcgaccgtt tcgcccggca tgccgatgat gaagtaagcc
      121 cagttcttga tgcccagctt atggctggtc tccagcgctc ggcgggtctt gtcgaagttg
      181 atccccttgc gcatccgctt gaggacgtcg atcgagcccg actcaagccc ccaggagagc
      241 atccagaagc cggccttcgc catccaggtc agcatctcct cgtcgacgaa atcgacgcgg
      301 ctgttgctgg tcagccggaa cttggtgcca tcctcgacga tcagcttgca gaggtcgatg
      361 acgacctggc gatcgacggt gaacaggtcg gcgtagaagt ggatgttggt gacgcccagc
      421 gaggcgagca ccttcatctc ttccagaatg cgctcggccg agtgggagcg gaccgtgttc
      481 tgccacatca catgcttgat gcagaagcgg cagccggccg ggcaaccacg gctggtgacg
      541 acgaagcagt acgggccgcg aatcatcggg atgcggtact tctgcaacgg cagcttgtgg
      601 tacagcggga tcggcaggtc atcgaggttg gcgatgaacg gccggtccgg gttggcgacg
      661 acctcgccct cgtggcggaa gacgcagccc tggacgccat gcagtgtctc acccgcctca
      721 agcttctgga tgatctcgcg cagcgtgaac tccggctcgt ggcggatgac catatcaagc
      781 gaggggtaat cagccagtgt ctcaaaggcc atcggcgtca catgcgagcc gatcgcgatc
      841 gtgaccgtcc cgatcgactt agcaatgtag gtcgtcgtca aatcgttctt gatcgtcggc
      901 gccgttacat tggtgacgag gaacttcggc cgccagtact ccagcttggc ctggaacgtc
      961 ggccagtcca tatcctcggc gatgcagtca atgatctcgg tcgtgtagcc ggcctcctcg
     1021 acgacggcgg ccagcccggc cagctccgtc tgcggccaga tcatcttctc gcgcgagcgc
     1081 cgcccgacgc ggtggatatc gcggatgaac acctcgccat ccggcgacgg cgggttgacg
     1141 aacaggacat cgaccagccg cttgaggtcc ggcgcgaagt ccttgacgct cgtctggctg
     1201 atctgcatca ggtcaacctt gccgaccttc ggcacgacct tcagcatctg cttctcaacc
     1261 gtgacgctgt tcccgccagc cggtgtcaac gggatcgtca ccttcgggct ggctgggatt
     1321 gtctcggttg tgacgccgtt cttggtctct accatctgct tctccactat ctcttcgtac
     1381 agccctcccg gcacatggga aggttgggga agggtttcta cccaaaccag accggttcct
     1441 tgccgacccg ctcgacacgc gagccgagcg agttctccag gagctcctgc gcggcgcaga
     1501 agcccatctc catgacgtga tcggtgttga cgtagcggaa cagcgcctga cggccggcgt
     1561 tgatcaggtt gtcgtagtcg gccaggtggt aggccatgtg gccgatcttg gcttcgaaat
     1621 cgacatcgta ggtcgggtag gcgtgcgaca gtcgccggaa gaaatgtccc agcacctgtc
     1681 gctcctcgat caggccggtt gccgccagct cgcggacggc acgcgtgtac atctcgtcct
     1741 ccggcatcgt ccagatctcg tcgccgatgt cgcaggtgat ctcaagcgtc accgaggtct
     1801 tgccctccgg cgccgcgtcg tgggtgaagt tgcccatctc cgagatgcga ttgaagatca
     1861 tcgggccggg gaaataggtc cagtggtcgt cggtgacctt cggcttgtcg atctccagga
     1921 agaggaagat catcgcccgg tattgcagcg accgcgcggc ctgcagcact tcctcaggcg
     1981 gggccgggcg cagcagctgg accagcagcg gcagcggcag cgaggacacg caggcgtcac
     2041 actcctgccg gatcgtctcg ccgttgtgct ggtactcgac ggcgcggatc gcctggccgt
     2101 catgctcgac ggcgctgatc cgcgcgccgg tcagcagatt cgcgccggcg ttcgtggccc
     2161 gctcggccat cgcctcggcg atctggccga ttccattgcg cggataccag aagtcattct
     2221 tgaactcgga gtgatgaaag ccattgtcgc cacgacgcag cttcatcacc cgcagcaaca
     2281 ggtcccaaag atcgacgaca gcgacccgct gggcggccca gctggccgag agctggcgcg
     2341 gatcgcgccc ccagaccttg gccgtgtacg ggccgaaata gacatcgtag agccgccgcc
     2401 cgaagcgatt gacgacccag gtctcgaacg agtcgtccgg cgtcggccgg atgcgctgcc
     2461 gggcgcgggt gtacatgaag tccagcatcg acgtgacggc cagcgtcttc ggcaagttgc
     2521 tcagcaggtt cgtcgcgttg agcggataat cgtagaagtc acccatgaag tagacacgcg
     2581 tcgtccgccg ccgcccttcg aggtcgtcgc cgacgagatc cttgacggtc tcgatgatgg
     2641 cggggttcga gctgtggaag cgatgcgggc cgaaatccca gagatagcca tcgcggcgca
     2701 tcgtggcggc cattccgcca agcgccgggg cggcttcgta gagatcgacg cccatgccgg
     2761 cttcggccag cttccaggcc gcgctcagtc cggcgacacc gccgcccagg accgccacgc
     2821 gctggtgatg tgtgagctgc tccatgacgc ttcctttcag cgctttcgag ctggtcggat
     2881 caaccaaacc gggcggcaac tgctccgccc cggatgctgt gcagaatcgg tggtgcgagg
     2941 tcggccggcg gctcgacgtc ctcgatcgtc cggatcaacc gggccggaac gccgagggca
     3001 accgtgttgt cggggatgtc gcgggtgacg accgccccgg cgccgacgac ggcattgcgg
     3061 ccaacgcgga caccgtcggt caccacggca ccggcaccaa gccaggcgcc cgactcaatc
     3121 gtgatgccgc gtgtcttcaa cccctgctgc atgatcggga cacggctggt ctcgataacg
     3181 tggtcgacgg ccaggatctg aacgccaggt gcgatcagaa cgtcatcgcc aatctggact
     3241 ccaccctggc cgcgaatcac cgtctgctcg ccgacgatgc agcgcttgcc aatcgtgatc
     3301 ccggcgttgg gcaggccgcg gaagttgtag acgtgcagaa tcgcgccatg catcacgagc
     3361 gtgtcgtcgc cgatcgtgat cccgccgggg caggcgtgca ggtaggtgct gtggtcgagg
     3421 tagacaccac gtcccagccg gatgttctcc gcgtaacgca gccgaactga ctgctcgatg
     3481 gccggcgcac catccgcctg cagcatcaac cggtagccaa tgccacgcgc ggcgatgccg
     3541 ggcaacgacg gaatccagct gagcagtgag gtcagagttt cctgcttgag ataggcgcca
     3601 acccccgacg cctgcgtccc gatatacgct tgcacctgcg ccagcacaga tcgacattcc
     3661 ctcccgggcg ggccgccaga agcggcacga caaccagctg atggcagttc tcgactgctt
     3721 ctcagcatcc ccggcagggg tgacaggcga ggggacggtt catggcagga ggttgacaag
     3781 ttagacagtg gagggaaaaa gatgcccccg tgccgattcg acacaggggg cgacggtccc
     3841 gctatggcac gtacatcact gtgacgttca tctatatcaa tactaagagt gaaacaaggc
     3901 gggtaaatcg tgcggctcgc acggctccac cggtgcgttt cctgtcccta gcccttgcca
     3961 acctgcaaga cgggtgagga cagaccatcg gcggaaaacg ccgactgcag ttgagaccag
     4021 tcgggccacc agaactcacg cgcgggcttc gatggaagga tctgctttag atacggaagc
     4081 gccgcttcca gatcggcggc gatcgcgtcc ggcacggtct tgcggtcaga ccgcccggcc
     4141 atcttccgga ccgcatcgcg cccgcgcccg gtcaggacaa gcacggtccg gcagccggcc
     4201 cgcttgccgg cttcgacatc ggtctcggca tcgccaacga agacggaggc ggcgagatcg
     4261 atccctagct gctcggcggc gcgcaacagc aagcccggtt tgggcttgcg gcagtcacag
     4321 tcgtcctggg tcgtgtgcgg gcaccagtag atttgctcga tccgcgcgcc ctgccgcgcg
     4381 atctcggccg ccatgcgctg gtggatctcg tcgatgcgct cttcgggaat cagcccgcga
     4441 ccgacgcctg cctggttcgt gatgacgacg accggcagat cgagcgtgtt caggccccgt
     4501 aaggcagcaa acgtccccgg caggaagctg aactcctccc agctgcggac gtattgatcg
     4561 cggttttcgt tcagaacgcc gtcgcgatcg aggaacacgg caggacggcc cacgtttgcc
     4621 cccagaactg tgcgcgccac actcgccgag cagcgcctgt tcggtcccac cagcctacga
     4681 accgctacgt tcagactcgt gtcaggttcc gccgatccca ctgacattct gttcaggtca
     4741 ggatttgtct catgcagaaa tcatgcatct gccgagcgcg tgacgggttt gtcagcctcg
     4801 attcagccgc ccgggatgtt ccattcacca gcgccaacga tcatcaacgc cagacagtca
     4861 gcccgccgtc agctatcagc acgagttgga aacgagcagt ggacgtgtca tcgacaggtt
     4921 cagtcgaagg gggcatgcgc ggccagtccg gcgatttcgc cggccagcgg cggctatccg
     4981 tcgccgtcgc tggcctcaat ctcgatctgg ctgtcgcagg cgttgtcctg ctgatgctgc
     5041 tgctcggctt catccggttg gaccgctacc cagcaccctg gttcgatgag ggcatccatc
     5101 ttcaggttgc aaagaatctg gtcgcggacg gcacctacgc ggcccgttcg gcggatggaa
     5161 cgctcgacta cgcaccagcg atcggcgtcg ggccaacgct gctgctgccg gtcgccgctg
     5221 cccagcagct cttcggcacg agccttgagg ccagccgtac tgtcccggtt ctctatctcc
     5281 tgctggcgac
//
LOCUS       DASUGJ010000794         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1517961, whole genome shotgun sequence.
ACCESSION   DASUGJ010000794 DASUGJ010000000
VERSION     DASUGJ010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517961"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFV93_17960"
     CDS             <1..191
                     /locus_tag="VFV93_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5167298.1"
                     /translation="RKHGGLAVLGFPISEEFKEVNAQDGKTYTVQYFERARFEWHPEN
                     QPPWNILGGHLGRTVYEG"
     gene            complement(268..393)
                     /locus_tag="VFV93_17965"
     CDS             complement(268..393)
                     /locus_tag="VFV93_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167299.1"
                     /translation="MLAIVRVLGPHLFSVVGRFGFFIAAIGFSRYRRRLWRWFWE"
     gene            717..>2747
                     /locus_tag="VFV93_17970"
     CDS             717..>2747
                     /locus_tag="VFV93_17970"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5167300.1"
                     /translation="MSTNATSTTSSQPGSVSIVGRSREQRILHERLMAAIGGHGSLVL
                     VAGEAGIGKTALTEWLADLARRHGADVLRGNCYDLTLTPPYGPWLDALADLASDSPQR
                     TRGQPLALDVAAGSVTSQAVLFEQAEASLADAAAVRPLVLVLEDLHWSDPASLDLLRF
                     LARSIDRSGILLIATYRANEIRARDPLFRLLPLLIREGHAERLDLRALGEGDVQAFVR
                     QRYILTARDESRLISYLQRRTEGNPFYLSEVLRTLEVERLLQPAEDRWLVGELNRTPL
                     PPLIRRIVEGRLAQLDEDTRWLLEVAAVIGQEVPLDIWMATSGADRERIGTAVERGIE
                     ASLLVELAAVGVGFAHALVRETLYQQLVLPRRQRLHSRVAGLLLQRSDPPFSAVAAHF
                     GLAGDPRAIDWLVRAGQHALKMYAADDVVTAINRAQEMAGRFTCELPATAYRTRAAAL
                     VMLGKFDEARRDYSTVLERARASKDRHAEWQALIDLGMLWAERDFEQTGTFYRAALAL
                     ARATGDDATVAYSLNRIANLHVNQDDPDAAIPLHQEALAIFEASGDRQGIAGALDFLG
                     MASFLTGDWSASMQYFERAIGRLRELGDQQRLASSLISIIGCGGTLGRKISTPVFREP
                     AFWLRSGEEGLALSRKIGWSAIESFGLISLSGIAGVRGDLGRALRDAEAGLAI"
BASE COUNT          473 a          900 c          943 g          431 t
ORIGIN      
        1 ggcgcaagca cggcgggctg gccgtcctcg gattcccgat ctcagaggag ttcaaggagg
       61 tcaacgccca ggatggcaag acctacaccg tccagtactt cgaacgcgcc cgcttcgaat
      121 ggcacccgga gaaccagccc ccctggaaca tcctcggcgg acatctggga aggacggtgt
      181 acgagggata gggagggatt cgggacacgg gattcggggg cgggtgaatc cattcacgtt
      241 ccccgaatcc catgtcccga atcccgatca ctcccagaac cagcgccaca atcgtcggcg
      301 gtagcgggag aagccgatag cggcgataaa gaagccgaag cgtccgacca cgctgaagag
      361 gtgtgggcca agaacgcgca cgatcgcaag cacaacggac ctccggttgc gcggctcgtg
      421 ccgcgggcta gaacatcagg agcaacaggc cggcaccaca ggccaatgcc gaaccggcga
      481 cagccagcag gattgggagt gtgcgcgtct tcggctgctg cacgcgccgg cgatggacaa
      541 ggatcgggag cattgtcgcc tctcagcctg catcgctcgc agaggtcatg actgtatcgc
      601 accggcagac cggcaatcca gccacgcatc agataggagc gatttgacag gttccggtgc
      661 ttggagcatg ctggcagcat ccagacggtg acacatcgca gactcgggca gcccgcatgt
      721 cgaccaacgc cacgtcaacc accagttcac agccaggctc cgtttcgatc gtcgggcggt
      781 cgcgggagca acgtatcctg catgagcggt tgatggcggc gatcggcgga cacggcagcc
      841 tggtgctggt cgctggcgag gccgggatcg gcaagaccgc gctgaccgag tggcttgccg
      901 atctggcacg gcgccacgga gcggacgtgt tgcggggcaa ctgctatgac ctgacgctga
      961 ccccgcccta cggcccgtgg ctggacgccc tcgccgatct cgcgtccgac tcgccgcaga
     1021 gaacgcgcgg gcagccgctc gcgctcgatg tcgcggccgg atcggtcacg agtcaggcgg
     1081 tgctgttcga gcaggccgaa gcatccctcg cggacgcggc ggccgtgcgt ccgctggtgc
     1141 tcgtgctgga agatctgcac tggtccgacc cggccagcct cgacctgttg cgctttctgg
     1201 cgcgctccat cgaccgttcg gggattctgc tgattgcgac ctaccgggcg aacgagatca
     1261 gagcgcgtga cccgctcttc cggttgctgc cgctgctgat acgtgaagga cacgccgaac
     1321 ggctcgacct gcgggcactc ggcgagggcg acgtgcaggc gtttgtccgg caacgctaca
     1381 tcctcaccgc gcgcgacgag tctcgcctta tcagctacct tcaacgccgc accgaaggca
     1441 acccgttcta cctctcggaa gtcctgcgga cgctggaggt cgaacgcctg ctccagccgg
     1501 cggaagatcg ctggctggtg ggcgagctca atcggacacc gctgccgccg ctgatccgcc
     1561 ggatcgtcga aggccggctg gcacagttgg acgaggatac ccgctggctg ctcgaagtcg
     1621 cggccgtgat cggtcaggaa gttccgctcg acatctggat ggcgacgagc ggggccgatc
     1681 gcgagcggat cggcacggcc gtcgagcgcg ggatcgaagc gagcctgctg gtcgaactgg
     1741 cggccgtcgg cgtcggcttc gcgcacgcgc tggtacgcga gacgctctac cagcaactgg
     1801 tcctgccacg tcggcaacgg ctccactccc gtgtcgccgg cctgctgctg caacggtccg
     1861 atccgccgtt cagcgctgtc gccgcccact tcgggctggc cggtgatccc cgcgcgatcg
     1921 actggctcgt tcgggccggc cagcacgcgc tgaagatgta cgcggcggac gatgtcgtca
     1981 cggcgatcaa ccgcgcccag gagatggccg gccggttcac gtgcgagctg ccggcgacag
     2041 cgtaccggac gcgggctgcc gcccttgtga tgctgggcaa gttcgacgag gcacgccgcg
     2101 attactcgac ggtactggaa cgggcgcggg caagcaagga tcgccacgcc gaatggcagg
     2161 cactgatcga tctgggcatg ctgtgggccg agcgggactt tgagcagacc ggcacgttct
     2221 accgggccgc gctggcgctg gcccgtgcaa ccggcgacga tgcgacggtc gcctatagcc
     2281 tgaaccggat cgccaacctg catgtcaacc aggacgaccc ggacgcggct atcccgctcc
     2341 atcaggaagc gctggcaatc ttcgaagcgt ccggagaccg gcaggggatc gccggagcac
     2401 ttgacttcct cgggatggcg agcttcctga ccggtgactg gtccgccagc atgcagtact
     2461 tcgagcgcgc catcggacgg ctgcgcgaac tcggagacca gcagcggctg gcgtccagcc
     2521 tgatctcgat catcggttgc ggtggaacgc tcggccgcaa gatctccacg cccgtcttcc
     2581 gcgagccggc cttctggctg cgctccggcg aggaggggct ggccctgtcg cgcaagatcg
     2641 gctggtcggc gatcgagtcg ttcgggctga tcagcctcag cgggatcgct ggcgtgcgtg
     2701 gcgacttggg gcgggcgctg cgcgacgcgg aagccgggct ggcgatc
//
LOCUS       DASUGJ010000795         3139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1518102, whole genome shotgun sequence.
ACCESSION   DASUGJ010000795 DASUGJ010000000
VERSION     DASUGJ010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518102"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VFV93_17975"
     CDS             complement(<1..967)
                     /locus_tag="VFV93_17975"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5167301.1"
                     /translation="MAAGGSAAGTASPRGSIFTAIQIYPSFRLLWIGTVATYLGQWMQ
                     NIALGWQMLVLTDSAFWVGMIAFASGIPYLIVTLPAGALIDRVDERRLLLTAQWCAMI
                     VASGLAALILSGHAEPWHLIVAAALNGTIMAVIQMVRQTLVPALVSREHMANAVSLNS
                     AGSNAMRIIGPSIAGLIIGGFGAGVCFVLQALALVGALMTTTRIEPLENQRTGIAAGG
                     ILDGWYEVRKRPALGSLILITAIPSLLVFPYIQMMPVFARDVFDIGAGGLGLLMAASG
                     VGALTGALTAATMDRVARKGRMVMLLTVVYCLAVCGFAISPHPALA"
     gene            complement(967..>3139)
                     /locus_tag="VFV93_17980"
     CDS             complement(967..>3139)
                     /locus_tag="VFV93_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5167302.1"
                     /translation="RPDSGPKLTGRERYAADIRLPGMLDACFVLSSQASATIRGIDRS
                     AALAVEGVVAVITAEDLPEFARDDAPQDRGMFFLAHQRVVFVGQPVAVVLAESAAAAE
                     IAADLVEVEYDPLPIVAGPREARAADATAVRPTHPDNVSNQVRHTRGDVERAFREAAI
                     VVEREFHSHAVHQSYLEPRAVAAAADPTGGVTVYTPTQGQFAIRTAVAKALRLAETDV
                     RVQPLTVGGGFGGKFVLLEPLAALLALTCGRPVRLELTRSHDFVATTPAPEAILRVAL
                     AASADGILTGLRADITFDTGFFAHAPYQQAGLMVAANYRIANLDITSTEAFSNRTGAG
                     AYRAPGRTQTAFALETLLDEIAEQLDITPIELRLRNVVVTGDELPDKTFWPGIELVKL
                     LEAARAHPLWTTPCEDGEGVGIGLGSMVGTTESASATMRLNADGTLSVIVGSVDLTGT
                     TSGLTQIAAEVFGTDPARIRVMTAPAEVAPHAGGTGGSKILYTVGNATIGAARDAREQ
                     VLAIAADTLEAGPGDVEIDDDRVVVRGAPDRALTLAQVHARTVTMGSPYAPVLGRGTV
                     ANAAKAPPTTVHLARVRVDRETGRTQVTGYAAFQDVGRAINPAEIDGQIHGAVAQGIG
                     WALGEALIFDDDGQLVTGSFMDYALPHAADVPPIDTDIHEYPSEHGPFGAKGVGEIPV
                     VPPAAAIANAIANATGARPTELPMTPERVWRALAEREGRRR"
BASE COUNT          482 a         1106 c         1080 g          471 t
ORIGIN      
        1 tcgccagggc cgggtgtggg ctgatcgcga agccgcagac ggccaggcag tagacgaccg
       61 tcagcagcat caccatccgg cccttgcgcg cgacgcggtc catcgtcgcc gccgtcagcg
      121 cgccggtcag cgcgccgacc ccgctggccg ccatcagcaa cccgagcccg ccggcgccga
      181 tgtcgaacac atcgcgggcg aagaccggca tcatctggat atagggaaag acgagcagcg
      241 aggggatcgc cgtgatcagg atcagcgagc cgagcgcggg ccgcttgcga acctcgtacc
      301 agccgtcgag gatgccgccg gccgcgatcc cggtccgctg gttctcgagc ggctcgatcc
      361 gcgtcgtcgt catcagcgcg ccgaccagcg cgagtgcctg gaggacgaag cagacgccgg
      421 cgccgaagcc accgatgatc agaccggcga tcgaggggcc gatgatgcgc atcgcgttgc
      481 tgccggccga gttgagcgag acggcattcg ccatgtgctc acgcgagacg agcgccggga
      541 cgagcgtttg ccgcaccatc tggatgacgg ccatgatcgt cccgttcagc gccgccgcca
      601 cgatcaggtg ccagggttcg gcatgtccgg acaggatcag ggctgccagc ccgctcgcga
      661 cgatcatcgc gcaccactgg gccgtcagca gcaaccgccg ctcatcgacc cggtcgatca
      721 atgccccggc cggtagtgtc acgatcaggt acgggatacc ggacgcgaac gcgatcatcc
      781 ccacccagaa ggccgagtcg gtcaggacca gcatctgcca gccgagcgcg atgttctgca
      841 tccactggcc gaggtaggtc gcgaccgtcc cgatccagag cagccggaag gatgggtaga
      901 tctgaatcgc cgtgaagatc gacccgcgcg ggctggccgt cccggcggcg ctccctccgg
      961 ctgccatcag cggcgccgcc cctcgcgttc ggccagcgcc cgccataccc gctctggcgt
     1021 catcggcagc tctgttggcc gggcaccggt cgcgttcgcg atcgcgttgg cgatggctgc
     1081 tgccggtggg acgaccggga tctcgccgac gcccttcgcg ccgaacggac cgtgctcgga
     1141 tggatattcg tggatgtcgg tgtcgatcgg cggcacgtca gcggcgtgcg gcagcgcgta
     1201 gtccatgaac gagccggtga cgagctgtcc atcgtcgtca aagatgagcg cctcgccgag
     1261 tgcccagccg atcccctggg cgacggcgcc gtggatctgg ccgtcgatct cggccggatt
     1321 gatcgcccgg ccaacgtcct ggaaggccgc gtagccggtg acctgcgtcc gacccgtctc
     1381 gcgatcgacc cgcacccgcg ccagatggac ggtcgtcggc ggcgccttgg cggcgttggc
     1441 gaccgtcccg cgtccgagaa ccggcgcata ggggctgccc atcgtaaccg tccgggcgtg
     1501 gacctgggcc agcgtcagcg cccgatccgg cgcgccacgg acgacgaccc ggtcatcgtc
     1561 gatctcaacg tcaccggggc cggcttccag cgtgtcggcc gcgatcgcca gcacctgctc
     1621 gcgggcgtcg cgggcggccc cgatcgtcgc gttgccgacc gtatagagga tcttgctgcc
     1681 accggtgccg ccggcgtgcg gggcgacctc ggccggcgcc gtcatgacgc ggattctggc
     1741 cggatcggtc ccgaacacct cggcggcgat ctgggtcaga ccgctcgtcg tgccggtcag
     1801 gtcgaccgag ccgacgatga cgctcagggt gccgtccgcg ttgagccgca tcgtcgcgct
     1861 ggccgattct gtcgtaccga ccatcgaccc gagtccgatg ccgacgccct caccatcctc
     1921 acagggtgtc gtccagagcg gatgagctct agcggcctcc agcagcttga ccagctcgat
     1981 ccccggccag aacgtcttgt caggcaactc gtcgcccgtg acgacgacgt ttcgcagccg
     2041 cagttcgatc ggcgtgatgt ccagctgttc cgcgatctcg tcaagtaacg tctccagcgc
     2101 gaaggccgtc tgggtgcgtc ccggcgcccg gtaggctccg gcaccggtcc ggttgctgaa
     2161 cgcttcggtc gaggtgatgt cgagattcgc gatccggtaa ttggcggcga ccatcagccc
     2221 ggcctgctgg tacggcgcgt gggcgaagaa gccggtgtcg aacgtgatat ccgcccgcaa
     2281 tccggtcagg atgccgtcgg cgctggccgc aagtgccacc cgcaggatcg cctccggcgc
     2341 cggtgtcgtc gcgacgaaat cgtggctgcg ggtcagctcc agccggacgg gccgtccaca
     2401 ggtcagcgcc agcagcgcgg ccagcggctc cagtagcacg aacttgccgc cgaacccgcc
     2461 gccgaccgtc agcggctgga cgcgaacatc ggtctcggcc agccgcaacg ccttcgcgac
     2521 ggccgttcgg atcgcgaatt gcccctgggt cggcgtgtag accgtcacac cgccggttgg
     2581 gtcggcggcg gctgccacgg ctcgcggttc gaggtagctc tggtggacgg cgtgcgagtg
     2641 gaactcccgt tcgacaacga tcgcagcctc gcggaaggcg cgctcgacat cgccgcgtgt
     2701 gtggcgcacc tgattgctga cgttgtccgg atgggtcggc cgaacggccg tcgcgtccgc
     2761 cgcccgcgcc tcgcgtggcc cggcgacaat cggcagcggg tcgtattcaa cctccaccag
     2821 atcggcggcg atctcagccg ctgccgccga ctcggccagg acgaccgcca ccggctggcc
     2881 gacgaagacg acccgctggt gggccagaaa gaacatccca cggtcttgcg gtgcgtcatc
     2941 acgcgcgaac tccggcagat cctcggcggt gatgaccgcg acaacgccct cgaccgccag
     3001 cgcggcggag cgatcgatcc cccggatggt cgcgctggcc tggctggaga ggacgaaaca
     3061 ggcgtccagc atgcccggca ggcggatgtc ggcggcgtag cgttcacggc cggtcagctt
     3121 cgggccggaa tcgggccgc
//
LOCUS       DASUGJ010000796         3846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_270362, whole genome shotgun sequence.
ACCESSION   DASUGJ010000796 DASUGJ010000000
VERSION     DASUGJ010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270362"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFV93_17985"
     CDS             complement(<1..574)
                     /locus_tag="VFV93_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167303.1"
                     /translation="MSLIICGVFLVDLVAQNLAALDVFNPDAMGVRQVMRMLDLGAEA
                     SFGTWYQVIALSMLSMILGTIAIVKRQVDDRYTRHWFFLALLALGFSLDEQVRFHDPQ
                     GGTEVYRERLGLSGPIFYGWVLAGTLSVVVVWLIYRRFLADLPRTTRHLYMLAAGLFV
                     SGEIVMESLSGWYADISGSETDLIYQTLTSV"
     gene            complement(754..1971)
                     /locus_tag="VFV93_17990"
     CDS             complement(754..1971)
                     /locus_tag="VFV93_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167304.1"
                     /translation="MNAIAAWESEERLTAEQAIRLREELAQPQFVAVLPHFGVHLAIG
                     AVLRFPIGSMVRVSYVLANLLLATLRLLVRNIDRHVWHQAIRIHSPLVLLISGMPGIG
                     TFAYLASKPMRSNHLLVRVGVDAVLLKLPRHVYERIGLRWVIALPSRTESQTLAATEK
                     PLQITVWPPNVVLILAMIVGALSAADALVVIADTMLHPSIPGWEYINRILDLNAESSL
                     GTWYAGASLILLSGLLFSIAIDKLHSGDRYGRHWLVLAFLPLGFSIDEQAKLHDPGGL
                     ANSVRDRLPLSGPLYTGWVIFGIISVIVVAMLFKGFLLDLPQSTRRLYMLAAALFIAG
                     ELVFEMMSGWYLDRSGSEDFVYQILTTFEESLSMAGIFVAIAATLHYIQVHLGEVRIA
                     LHGIRPAVEGVQR"
     gene            complement(2085..3059)
                     /locus_tag="VFV93_17995"
     CDS             complement(2085..3059)
                     /locus_tag="VFV93_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167305.1"
                     /translation="MATVDTGLQLGDPESASADERRHGPPGHRPSRLLRVSHDIYWRI
                     HHVLVTVPLVHAAYVPHDESKPHAHALTSTILFNGNTVSKIYAPGLVPRAIYWLAFQA
                     PFPYMRNEAALRAAILRRNLAGMLTEYWYGSNRVARAIAIDQIDGRSAITSEYVPGGP
                     PTDRHAAHAFLDDLARRFDAVGLPTWQVDPRQPRAMDNLLETPDGVYHIIDLESGLVS
                     PLASPRAWWRAFQRGLVPIYDDVYFDLTRAYVDREAPSIREAKGDHWLEELRRLLDEA
                     EAETRAWHAGEPRIWSRFVQRVIGLLHRPGRTATRSLPTTDETNGGTP"
     gene            complement(3324..>3846)
                     /locus_tag="VFV93_18000"
     CDS             complement(3324..>3846)
                     /locus_tag="VFV93_18000"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167306.1"
                     /translation="LGLAAGLLLGIVAFPLPGNLTLKLGLAGGPLIVALVLGTLERTG
                     PFVWGIPYSANLTIRQLGLILFLAGVGTRSGRAFVETAFSRDGLLLLLTGAAVTTTVA
                     VLTLWIGYRLLRVPMGLLIGILAGIQTQPAVLGFALEEAGDELPNIGYAIVYPVATIG
                     KILLAQLLLSIWL"
BASE COUNT          779 a         1148 c         1247 g          672 t
ORIGIN      
        1 cgacgctcgt caaggtctgg tagatcaggt cagtctcgct gcccgagatg tcggcgtacc
       61 agccagacaa gctctccatg acgatctcgc cactcacgaa tagcccggcc gccagcatgt
      121 acagatgccg cgttgtgcgc ggaaggtcgg cgaggaaccg gcggtagatc agccagacga
      181 ccaccaccga gagcgttccc gccagtaccc aaccgtagaa gatcggtccc gagaggccga
      241 gtcgctcgcg gtacacttcg gtgccaccct gcgggtcatg gaagcggacc tgctcgtcga
      301 gcgaaaagcc gagtgcaagc aacgccagga agaaccagtg gcgtgtgtac cggtcgtcga
      361 cctgccgctt cacaatcgcg atcgtgccca ggatcatcga gagcatcgac aaagcgataa
      421 cctgatacca ggtgccgaac gatgcctcgg ctcccaggtc aagcattcgc atgacctggc
      481 gcacgcccat cgcgtcggga ttgaagacat cgagcgcggc cagattctgt gcgacaaggt
      541 caacgagaaa cactccgcag atgatcaggc tcaacgtgcg ggcgattcct ggtgtccaga
      601 ctgacagtct gtgccgtgat tggtcacgtc ccgacgtctg aatcgtttcc gccggctgac
      661 cgaacaccgc gttcaggccc gcatgctcag cgatgttgag atgccagatc tttagcgtgg
      721 gatcgcgccc ctcatgaagt cgccaatgcg ttctcatcgc tgcactcctt caacggccgg
      781 acgaatcccg tgcaacgcga tacgcacttc gccaaggtgg acctggatgt aatgcaacgt
      841 cgcggcgatc gcgacgaaga tgccggccat cgagaggctt tcctcgaagg ttgtcaggat
      901 ctggtacacg aagtcttcgg agccggaccg gtcgagatac cagcccgaca tcatctcaaa
      961 gacgagttct cctgcgatga agagcgcggc cgcaagcatg tacaaccggc gagtcgattg
     1021 aggcaaatcc aacagaaatc ccttgaacag catcgcgacg acgatgactg agatgatccc
     1081 gaagatcacc catccggtat agagcggccc gctcaaggga agtcgatcgc gaacgctgtt
     1141 cgcgagtcct ccaggatcgt gcaacttggc ctgctcatcg atcgagaatc ccaatggcag
     1201 gaacgcgaga acgagccagt gcctgccgta tcggtcgccc gagtgaagtt tgtcgattgc
     1261 gatgctgaag aggagtccgg acaagaggat gagcgacgca ccggcgtacc atgtccccaa
     1321 cgacgattcg gcgttcaggt ccagaatacg attgatgtat tcccagccgg gaatgctcgg
     1381 atgcagcatc gtatccgcga tgacgaccag cgcgtcggct gcgctcagcg cgccgacgat
     1441 catcgccaga atcaggacaa cgttcggcgg ccagaccgtg atctgcaacg gcttttcagt
     1501 cgccgcaagt gtctgcgatt cggtgcgaga tgggagggcg ataacccacc gtaacccgat
     1561 ccgctcgtag acgtgcctgg gcagtttcaa caggacggcg tcgaccccga cacggaccag
     1621 aaggtgattg gagcgcatcg gcttcgaggc caggtaggca aacgttccga taccgggcat
     1681 tccggagatc agcaacacca gtggtgagtg aatgcgaatc gcctgatgcc agacgtgccg
     1741 gtcgatgttg cgcaccagga gccgcagtgt cgcgagcaat aggttcgcga gcacgtagct
     1801 cacgcgcacc atgctgccga tcggaaatcg aagcactgct ccgatcgcca ggtggacgcc
     1861 aaagtgaggc agcactgcga cgaactgcgg ttgtgccagc tcctcgcgaa gccggatggc
     1921 ttgttcagct gtcaggcgct cttcggattc ccacgccgcg atggcgttca tgaaccagcg
     1981 cgttgcccgg ctctgacgct gttcctggga ctcgcgctct ctgtgaaccg gcatgtggac
     2041 gcggccgagg cgacgatggc ggtgttccct ctggtccagg gtggtcatgg tgtccccccg
     2101 ttcgtctcat ctgtcgtggg tagggaccgg gtcgcggtcc tgcccggccg atgcagtagt
     2161 ccgatcacgc gctgaacgaa ccggctccag atacgcggct caccagcgtg ccaggcacgc
     2221 gtctccgcct cggcctcgtc gagcagacgg cgaagctcct ccagccagtg atcgcctttg
     2281 gcttcacgga tcgacggtgc ctcgcgatcg acatacgcgc gggtgaggtc gaagtagacg
     2341 tcgtcgtaga tcggcaccag cccgcgctgg aacgccctcc accaggcacg cggtgaggca
     2401 agtggcgaca cgaggcccga ctcgaggtcg atgatgtggt agacgccgtc gggcgtctca
     2461 agcaggttgt ccatcgctcg cggctggcgc ggatcgactt gccaggtcgg caggccgacg
     2521 gcgtcgaagc ggcgggcgag gtcgtcgagg aaggcgtgcg cggcatgccg gtccgtcggc
     2581 ggaccaccgg ggacgtactc actcgtgatc gcggagcggc catcgatctg gtcgatagcg
     2641 atcgcccgtg cgacgcggtt gctgccgtac cagtattcgg tcagcatgcc ggccaggttg
     2701 cggcgcagaa tggccgcgcg cagggcggcc tcattgcgca tataggggaa cggagcctgg
     2761 aacgccaacc agtaaatcgc cctcggcacg agcccaggag cgtagatctt cgagacggtg
     2821 ttgccgttga agaggatcgt gctcgtcagc gcgtgcgcgt gtggtttgga ctcgtcgtgc
     2881 ggaacgtagg cggcatggac gagcggaacg gtgacaagaa cgtggtggat tcgccaatag
     2941 atgtcgtgcg agacacgcaa gagccgggac ggtcggtgac cgggagggcc gtgacgccgt
     3001 tcgtcggcag atgctgattc aggatcaccc agttgaagtc ccgtatcgac ggttgccatc
     3061 gcgtcgactc ttctcttatc tggatggcga gcgattcgcg accgcgacgc catcgcgcct
     3121 catcatcgac gtgatggtat gccgcgagcc attgcaggca cgtggatgcg tcgcgtccag
     3181 tgtgtcaagt ccgtgacagg cggtgatcag ggttctgcaa tgtgtcgagg ggccacgccg
     3241 attgatgggc agctgatgtg tatggctgga acgtctcgca ggcgggtagt cgagcggact
     3301 aaccctttca gcggtaatcc cggctatagc cagattgaca ggagcagttg tgcaaggaga
     3361 atcttgccga ttgttgcgac cggatagacg attgcgtagc cgatgttcgg aagttcatcg
     3421 ccggcctctt caagtgcgaa gccgagcacg gctggctgcg tttggatacc ggccaggatg
     3481 ccgatcagga ggcccatcgg cacgcggagc agccggtagc cgatccagag ggtcagaact
     3541 gcgaccgtcg tggtgaccgc cgcgccggtg aggagcagca gcagcccatc gcgactgaat
     3601 gcggtctcga cgaacgcgcg acctgaacgc gtgcccaccc cagccagaaa caggatcaaa
     3661 ccgagttggc ggatggtcag gttggcgctg tagggaatgc cccaaacgaa cggaccagtg
     3721 cgctccagcg ttccgaggac gagtgccacg atgagtggac cgcctgccag gccgagcttc
     3781 agcgtcaggt ttcccggcaa cgggaatgcc acgatcccga gcagcagacc ggctgccagc
     3841 ccgagg
//
LOCUS       DASUGJ010000797         5680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1935657, whole genome shotgun sequence.
ACCESSION   DASUGJ010000797 DASUGJ010000000
VERSION     DASUGJ010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935657"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFV93_18005"
     CDS             <1..190
                     /locus_tag="VFV93_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HEX5167307.1"
                     /translation="AETARPIFAALRELRESMQARYFESDLANLSMGMTNDYVAAIEE
                     GATIVRVGRAIFAETASS"
     gene            190..972
                     /locus_tag="VFV93_18010"
     CDS             190..972
                     /locus_tag="VFV93_18010"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5167308.1"
                     /translation="MRVVSLGSGSSGNGFLLDTGEVALLIDCGVAVRACQSAIRDLGV
                     ADRLAGIVVSHEHIDHVRSIPSITRKLDIPLITTIGTHGALRTTLRLDRRAAGERYSN
                     GSLEVTFFGVSHDAAEPCGFVAEHDGTRVAVFTDLGHVSQAVLEAIASADIVVLEANY
                     DRQMLMQGPYPAHLKRRIQGPAGHLGNDDCATALAATVSDRTQAIWLAHLSDKNNLPD
                     IAIGTVREAIALMGYDAPVNALPRFERAEITRVPARQLQFGL"
     gene            complement(1039..2853)
                     /locus_tag="VFV93_18015"
     CDS             complement(1039..2853)
                     /locus_tag="VFV93_18015"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5167309.1"
                     /translation="MRTAADPDDLLGKAYDSRIAGRLVGHVRPYRRDAWYVIFLVIVT
                     TIMDLAVPYLFGQAVDIVNREEGHTFLGQTGSSALNLIALAFLAVILLRFLGRYRELF
                     VMSRLGQKIVYDLRSEMFRHIQKVGVRYIDRRGVGSIMSRLQNDVAVIDQLFTDGLID
                     IIAQLAILIGIIILMLVTNLKLALLAFAVLPFMVVLMIFWRRRAIMTYRQTRITIARV
                     NAFLAENIAGVRVIQSFTREPINRNQFRVINDDNLDANIGAARLSALLFPFVTFVEAL
                     ATALVLFVGGRMVLGADAFTIGELVFFVALIGRFYEPINQLSQGYNTMQAAMAAGERI
                     FDILDVDVEIEDKPNATVLPRIRGRVEFDHVRFGYADKEVLHDISLDVAPGESIAFVG
                     ETGAGKTSMINLISRFYDVWDGAIRIDGYDVRDVTQQSLRSQLGVVLQDTFLFAGTIG
                     ENIRFARPSATDEEIIAAAKAVGAHEFIMNLPGGYDAEVHERGATLSVGQRQLLSFAR
                     ALIADPRIIILDEATSSVDTETELVIQSALRRLLAGRTSFIIAHRLSTIREASRVVVM
                     DQGRIIEIGSHEELLDRRGAYYNLYTMQFRSEDAEAAD"
     gene            complement(2872..4578)
                     /locus_tag="VFV93_18020"
     CDS             complement(2872..4578)
                     /locus_tag="VFV93_18020"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5167310.1"
                     /translation="MTLVVAYVSLFIAISAQLTVPKLIGRVIDDGIAVNDRSVILSGA
                     ALVIGATAIQAIFTYVRTFLFQSMAEHVATDTRRQLYEHMLTLSFSFFDTSQSGQLMS
                     RATEDVNAIRRFMMFSLRMAIYSVSMLVIITVLLLRAHLTLALISLAAMPFLAWTALH
                     FSTTIRPMFARTQQQFGEMSSVLQENLAGTRVVRVFAREDDEVTKFDNSIRTLMSRQM
                     DAIRYYSFYFPFMQLISAVSLLAILWYGGRQVMNGSISVGTLVTFNIYLTLLAMPIRQ
                     LGWIMNSVARAIASGERIFEVIDSRPVIRDSPNAIELAEPRGEIRLEDVTFTYPHAPR
                     PALTNVSVDAGPGEVIGLIGTTGSGKSSLTALVARFYDVSSGRITFDGVDIRDLKLSF
                     LRRNVGFVMQETFLFSTTIRDNISFGNPDVSDEQIQTAARMARAHDFIMALPDGYETR
                     LGERGTSLSGGQRQRVAIARALCSDPRVLILDDATSSVDTETEWEIQQALRNAMEGRT
                     TFIIAQRVSTLKDASEILVLEDGAVVERGTHLSLIEADGVYARLYELQLKNQEELAQA
                     AD"
     gene            4834..5367
                     /locus_tag="VFV93_18025"
     CDS             4834..5367
                     /locus_tag="VFV93_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEX5167311.1"
                     /translation="MEAERNEYQETIDEALRIVYSHHHRLVTKLFPDAERPLNMESLR
                     SGPLGRDLRKLAALARGEIREQKELVLERIETVLQLLFWPPMADDYTVPRSFWETPLG
                     RMMSMAKYRAYEPSELVSIGTAAQKLGVTRPTIYRWMDERHLGYVRDEMSGRTFVVQR
                     DVDSLLNDSQAAGFPSA"
     gene            complement(5432..>5680)
                     /locus_tag="VFV93_18030"
     CDS             complement(5432..>5680)
                     /locus_tag="VFV93_18030"
                     /inference="COORDINATES: protein motif:HMM:NF013038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5167312.1"
                     /translation="QTVVSVPEDTAEGDIVRVIGDGSLGEMTFDDIASMIGTVNYEVA
                     TRVMARVPRIYLRGGKPVAWELVLTGERDSIEVAAVSR"
BASE COUNT         1104 a         1760 c         1821 g          995 t
ORIGIN      
        1 cgccgagacc gcccggccca tcttcgccgc gttgcgcgag ttgcgcgagt ctatgcaggc
       61 acgctatttc gaaagcgacc tcgccaacct ctcgatgggc atgactaacg actatgtcgc
      121 cgcgatcgaa gaaggcgcga cgatcgttcg ggtgggccgc gcgatcttcg ccgagacggc
      181 cagcagctga tgcgggtcgt ctccctcggc tcaggcagca gcggcaacgg ctttctgctc
      241 gacaccggtg aggtcgcgct gctgatcgac tgcggcgtgg ccgttcgtgc ctgccagtcc
      301 gcaatccgcg acctcggcgt ggccgaccgg ttggctggca tcgtcgtcag ccacgagcac
      361 atcgaccacg ttcgctcgat cccgtcgatc acccgcaagc tggatatccc gctgattacg
      421 acaatcggca cccacggcgc actacgaacc acgctgcgcc tcgaccggcg agctgccggc
      481 gagcgctaca gcaacgggtc gctggaggtt acgttcttcg gcgtctcgca cgacgccgcc
      541 gagccgtgcg gcttcgtcgc cgagcacgac ggcacccgcg tcgcagtctt caccgacctc
      601 ggccacgtca gccaggccgt gctggaagcg atcgcaagcg ccgatatcgt cgtcctggag
      661 gcgaactacg accgccagat gctgatgcag ggaccatacc cggctcacct caagcgacgc
      721 atccaggggc cagccggcca cctcggcaac gacgactgcg cgaccgcgct ggcggcaacc
      781 gtctcagatc ggacccaggc gatctggcta gcacacctct cggacaagaa caacctgccc
      841 gacatcgcta tcggcaccgt tcgtgaggca atcgcgctga tgggatatga cgccccggtc
      901 aacgcccttc cccgcttcga acgtgccgag atcacgcgcg ttcccgcgag gcagcttcag
      961 ttcggtctgt aacgccatca caatccccga tccggtaggg gattggtgtt tcgattgagt
     1021 tcgttcacgg cgttgccgtc agtcggcggc ctcggcgtcc tcggagcgga attgcatcgt
     1081 gtagaggttg tagtaggcgc cgcgacggtc gagcagctct tcgtggctgc cgatctcgat
     1141 gatccggccc tggtccatca cgacgacgcg gctggcttcg cggatcgtcg agagccggtg
     1201 ggcgatgatg aacgacgttc ggccagccag caagcggcgg agagcagact ggatcaccag
     1261 ttccgtctcg gtgtcgacgc tggaggttgc ctcatcgagg atgatgatgc gcgggtcggc
     1321 aatcagcgcg cgggcgaacg aaagcagttg ccgctgaccg actgagaggg tcgctccccg
     1381 ttcgtggacc tccgcgtcgt atccaccagg gagattcatg atgaactcgt gcgcgccgac
     1441 cgccttggca gccgcgatga tctcttcgtc ggtggcagac gggcgagcga agcggatgtt
     1501 ctctccgatc gtcccggcaa acaggaacgt gtcctggagg acaacgccga gctgcgagcg
     1561 gagcgactgc tgcgtgacgt cacgaacgtc gtagccgtca atgcggattg cgccgtccca
     1621 gacgtcatag aagcgcgaga tgagattgat catggaggtc ttgccggcgc cggtctcgcc
     1681 gacgaaggca atcgactcgc cgggcgcgac gtcgagcgag atgtcgtgga ggacctcttt
     1741 gtcggcatag ccgaaacgga cgtggtcgaa ttcgacgcgg ccccgaatgc gcggcaggac
     1801 agtggcgttc ggcttgtcct cgatctcgac gtcgacatcg aggatgtcga agatgcgctc
     1861 gccagccgcc atcgcagcct gcatcgtgtt gtagccctgc gagagctggt tgatcggctc
     1921 gtagaagcgg ccgatcaggg cgacgaagaa gaccagctcg ccgatcgtga aggcatcggc
     1981 gcccaacacc atccggccac cgacgaacag caccagcgcc gttgccagcg cctcgacgaa
     2041 ggtaacgaat gggaacagca gcgccgatag acgcgccgct ccgatgttgg cgtccaggtt
     2101 gtcgtcgttg atgacccgga actggttgcg gttgatcggc tcgcgggtga acgactggat
     2161 cacccgaacg ccggcgatgt tctcggccag gaaggcgttg acgcgggcga tcgtgatgcg
     2221 tgtctgccgg taggtcatga tcgcacgccg ccgccagaag atcatcagga cgaccatgaa
     2281 cggtaggacg gcgaacgcca gcagcgccag cttgagattg gtcaccagca tcaggatgat
     2341 gatgccgatc aggatcgcga gttgagcgat gatgtcgatc agaccgtcgg tgaagagttg
     2401 gtcgatgacg gcgacatcgt tttgcaggcg cgacatgatc gaaccgacgc cgcgccggtc
     2461 gatgtagcga acgccaacct tctggatgtg acggaacatc tcggagcgca agtcgtagac
     2521 gatcttctgg cccagccggg acatgacgaa gagttcccgg tagcggccga ggaagcgcaa
     2581 caggatgacg gccaggaagg ccagcgcgat caggttgagc gcagacgaac cggtttgacc
     2641 gaggaaggta tgaccctctt cgcgattgac gatgtcaacc gcctggccga acaggtaggg
     2701 cactgccagg tccatgatcg tcgtgacgat gacgaggaag atgacgtacc aggcgtctct
     2761 gcggtagggt cgaacgtggc cgacgaggcg gccggcaatt cggctgtcgt aggctttgcc
     2821 taacagatcg tctgggtcag ctgccgttcg catcggtgtt cgccgccgga gctagtctgc
     2881 cgcctgggcg agttcctcct ggttcttcag ttgcaactcg tagagccggg cgtagacgcc
     2941 atcggcttca atcagcgaca ggtgcgtccc gcgctcaacg acggcgccat cttcgaggac
     3001 cagaatctcg ctggcgtcct tgagcgtcga gacacgctgg gcgatgatga acgtcgttcg
     3061 gccctccatc gcattgcgca gtgcctgctg aatttcccac tcggtctcgg tgtcgacact
     3121 cgatgtcgca tcgtcgagga tcagcacgcg tggatcgctg cagagcgcgc gagcgattgc
     3181 cacacgctgc cgctgtccac ccgaaagcga cgtgccgcgc tcgcccagcc gcgtctcata
     3241 accgtccggc agcgccatga tgaagtcgtg ggcgcgagcc atccgggccg cagtttgaat
     3301 ctgctcgtcg ctgacatcgg gattgccgaa ggagatgtta tcgcgaatcg tcgttgagaa
     3361 caggaacgtc tcctgcatga cgaacccgac gttgcgccgc aggaacgaga gcttcaggtc
     3421 gcggatgtcg acaccgtcga acgttatacg gcccgaactg acgtcgtaaa agcgggcgac
     3481 aagcgccgtc agcgagctct tgccactgcc ggttgtcccg atcagcccga tgacctcacc
     3541 ggggccggcg tcgacgctga cgttcgtaag ggccggtcgc ggtgcgtgag gataggtgaa
     3601 ggtgacatcc tccagccgga tctcgccgcg cggctcggcc agctcgatcg cgttcggcga
     3661 gtcgcggatg accggccggc tgtcgatgac ctcgaagatg cgctcgcccg aggcgatcgc
     3721 gcgggcgacc gagttcatga tccagccgag ctggcggatc ggcattgcca gcagcgtcag
     3781 gtagatattg aaggtcacca gcgtaccgac tgagatcgac ccgttcatga cctggcgacc
     3841 gccgtaccag aggatcgcaa gcagcgagac agcggagatg agctgcatga acgggaagta
     3901 aaacgagtag taccggatcg cgtccatctg acggctcatc agcgtccgaa tcgagttgtc
     3961 aaacttcgtg acctcgtcgt cctcgcgggc aaagacacgg acgacgcgcg tgccggccag
     4021 attctcctgc aacacactcg acatctcgcc gaactgctgc tgggtgcggg cgaacatcgg
     4081 ccggatcgtc gtcgagaagt ggagggccgt ccaggccagg aacggcatcg ccgccagtga
     4141 gatcagcgcc agcgtcaggt gcgcccggag caacagaacg gtgatgatga ccagcatcga
     4201 gacgctgtag atcgccatcc gcaacgagaa catcatgaag cggcgaatcg cgttgacatc
     4261 ctcggtcgcg cgtgacatca actggccgga ctgcgacgtg tcaaagaacg agaacgacag
     4321 cgtcagcatg tgctcgtaga gctggcgacg ggtgtcggtc gctacgtgct cggccatcga
     4381 ttgaaacagg aacgttcgaa cgtaggtgaa gatcgcctgg atagcagtcg cgccgataac
     4441 cagcgcggca ccactgagga tgacgctgcg gtcgttgacg gcgatgccgt cgtcgatgac
     4501 gcggccaatc agcttcggaa cggtcagttg ggcgctgatt gcgatgaaca ggctgacgta
     4561 ggcgacgacg agcgtcaccc tgtaaggtcg aagataaccc aggattcgcc agatgattcg
     4621 cacgagtcgc ccccctgccg aacctggcca ggcccggcgc atccgctcag tatagcacca
     4681 cttgtgttct gtgcctcatc ataccgctac tggttgtacg ccgcgtacga atacgggtaa
     4741 ttctgttgaa aacgattgac ggaattcgag aagtgcagat acaatgtgat cgtacagggg
     4801 gaacacaccg tgcgccgcgc gaaaggagct ggcgtggagg ccgaacgcaa cgagtaccag
     4861 gaaaccatcg acgaggcctt acgtatcgtc tatagccatc atcaccggct ggtcacgaag
     4921 ctgttcccgg acgcggaacg gcctctgaat atggagtcgc tccgttcagg gccgcttggc
     4981 cgtgacctgc gcaagctcgc cgctctggct cgcggagaga tccgcgaaca gaaggagctc
     5041 gtcctcgaac ggatcgagac cgttctacag ttgctcttct ggccgccaat ggccgacgac
     5101 tacaccgtgc cccgctcgtt ctgggagacg ccgctgggcc ggatgatgtc gatggccaag
     5161 taccgcgcct atgagccgtc ggaactcgtc tccatcggga cggccgcgca gaagctgggc
     5221 gttacccggc cgacgatcta ccgctggatg gatgagcgcc acctcggcta tgtgcgcgac
     5281 gagatgtcgg gcaggacgtt cgtcgtccag cgtgatgttg actcgctgct gaacgacagc
     5341 caggccgccg gcttcccatc cgcctgacgc ggcgatcgcg cggcatcctt cctgaatccc
     5401 gtcggggcac ctttgggtgc cccgaagcgt gttacctcga aacagcagcg acctcgatgc
     5461 tatcccgttc gcccgtcaac acgagttccc aggcaaccgg tttgccgccg cgaagataga
     5521 tgcgcgggac gcgcgccatc accctcgtcg caacctcgta gttgacggtc ccgatcatcg
     5581 acgcgatgtc gtcgaaggtc atctccccca gcgacccgtc gccgatgacg cgcacgatat
     5641 cgccttcagc cgtgtcctcg ggaaccgaga cgaccgtctg
//
LOCUS       DASUGJ010000798         5056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_407936, whole genome shotgun sequence.
ACCESSION   DASUGJ010000798 DASUGJ010000000
VERSION     DASUGJ010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407936"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..376)
                     /locus_tag="VFV93_18035"
     CDS             complement(137..376)
                     /locus_tag="VFV93_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167313.1"
                     /translation="MKAYEARRSAEVNREQAVVASEATAARPAAAARTAPPNRRVYAL
                     SRAEEYAIIRSDLRRLLWILAVLTATLLVLTFFLR"
     gene            complement(493..1308)
                     /locus_tag="VFV93_18040"
     CDS             complement(493..1308)
                     /locus_tag="VFV93_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX5167314.1"
                     /translation="MAAANGIVTLMTDFGTVDGYVAAMKGSLLGVNRNVTIVDVTHHI
                     PPQDITEAAFQIATVWSAFPPGTIHVIVVDPGVGGERRAVVVEAGGQFFVTPDNGVLT
                     LLTANVVPGEVVVLDRPEYFLADVSSTFHGRDIFAPVAGHLSTGSVALAELGTPAPTD
                     SLVALPWAPIQDTPSRIHAPVVSIDRFGNCRTLIARRHLTADADLIYVRCGDVTVKGI
                     HRTYGDVPVGKTMALFGSHGGLEIAVRGGSAAQSWEIRRGDPVVVFTGDSFEP"
     gene            complement(1351..1743)
                     /locus_tag="VFV93_18045"
     CDS             complement(1351..1743)
                     /locus_tag="VFV93_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167315.1"
                     /translation="MTERDYSKFIRDSDEGPRLNVKLIPRAVYVEMVKRWRSRKDVIE
                     VVAEEGYWLWDLKELEQQALAEGREVSWAELAEEVAGDMDDREWWKTTAAFEEHLITH
                     FPEDPLAWSDLLDAVYDMQEQEGWIRRQ"
     gene            complement(1740..2411)
                     /locus_tag="VFV93_18050"
     CDS             complement(1740..2411)
                     /locus_tag="VFV93_18050"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX5167316.1"
                     /translation="MSTTLTHQTTRAELSSAILTGGASRRMGSDKALLSVAGTTLLER
                     AIRSVERVAGDIFIVGDRQPYRKFGVPVVADAYPGSGPLGGIATALRQARYEHVLVVA
                     CDMPFLSVALLEAMANERRDYDVLVPVRGQQADVQGGMRTYETLHAIYRRVCLPHLKQ
                     RLAQSELKVADALAGLRVRELPERWLRQHDPELLSFVNANRPEEWAAARRRLEEQVST
                     VEDQE"
     gene            2448..2984
                     /locus_tag="VFV93_18055"
     CDS             2448..2984
                     /locus_tag="VFV93_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167317.1"
                     /translation="MSIGCCSLPKQSATLSGINRVARWKVARDERRSKSRDGDCLSTR
                     LTPWCFLLSITSPDPASPQPLPSRTPIRGARVAQMTARGELPVNSTMISKIEKARRYA
                     EEPERVKFERFEVRFQGTHDEYTVKMDGSEFTCTCHFFEVQQMGTCCHIMALQRMFET
                     MMTEEQHTAGAPFSFATA"
     gene            complement(3032..3283)
                     /locus_tag="VFV93_18060"
     CDS             complement(3032..3283)
                     /locus_tag="VFV93_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167318.1"
                     /translation="MRSRVSRCLVQYYTALRLSAGAAMLLLPFEAPEPRTPVVRASDM
                     TGPSGFDRREMIVQAGRVSIVTMPHETERAPAQPAFRLN"
     gene            3407..4216
                     /locus_tag="VFV93_18065"
     CDS             3407..4216
                     /locus_tag="VFV93_18065"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5167319.1"
                     /translation="MSTAHRGRNLSDLTWIEASEVLVRRPIGLLPIGAIEAHGPHLPL
                     DTDVIIATATASRALDRLEAAGTPALILPPITYSVSFVGTSFAGTTPVEPDPFEAYLT
                     SILIQHLRQGYRAIVCCNAHLEPASVERVATASRSVTQRTGTPVLFPDQRLEPYVNLL
                     SDEFRAGARHAGGYETSIVMATRPDAVRMDRLAGLEPVWIDLPARLKEGAQTFAEAGA
                     TLGYFGEPATSTIEEGERMLDALAEIVMIALRDAGVDLSPDSRANVHPLSS"
     gene            complement(4285..>5056)
                     /locus_tag="VFV93_18070"
     CDS             complement(4285..>5056)
                     /locus_tag="VFV93_18070"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5167320.1"
                     /translation="DVIFHLAAQSYVPQAFAAPSDTISNNVTAQINLLEGCRAAGLDP
                     TILIVGSSEEYGQIAPEDVPLTEDQPFRPVNPYAVSKIAQDMLGLQYALSFGMRIVRV
                     RPFNHFGPGQSDRFVLATFARQVAEAELGRIEPVVLTGNLDARRDFLDVRDVVRAYRM
                     LIEFGTPGEVYNIASGVAHRVGDLLDRLIDQATIAVDVRLDPARTRPSDTPIVAGDAG
                     KLRSATGWEPLYSTDQSLRDTLDYWRTALASYVEPSIE"
BASE COUNT          938 a         1680 c         1568 g          870 t
ORIGIN      
        1 ctgccaggtg ccctctgggc ggtggtcgac cggcgaaaga tatcttgggc cgaaacgacc
       61 ggcaatcacc atccagagac gcgacgaggg cgacggttct accgtcgccc tcgttcgttt
      121 tccaggtctg cgccgcctac cgcaagaaga acgtcaggac gaggagtgtt gcggtcagga
      181 ccgccaggat ccagagcagg cgccgcagat cggagcggat gatcgcgtac tcttcggcgc
      241 ggctcaacgc atagacacgc cggttcggcg gagcggttcg agcggcggcg gccgggcggg
      301 cagcagttgc ctcgctggcg acgacagcct gctcgcgatt cacctcggcg gatcggcgcg
      361 cctcgtacgc tttcatctcc gctcgtgtca gttgccgtgg tgggcgggac ctggttggct
      421 tgctcaacga tagaccatcc tgcaacgata gaaactggat accattcaac agccgagtat
      481 atcagtttgc ggctacggct cgaacgaatc gccggtgaac acgacgaccg gatcaccgcg
      541 ccggatctcc cacgattgcg cggcgctacc acctcggaca gcgatctcca gcccgccgtg
      601 gctgccgaag agggccatcg tctttccgac cggaacatcg ccgtacgtgc gatgaatccc
      661 cttgacggtg acatcgccgc agcgcacgta gatcaagtca gcatcggcgg tgaggtggcg
      721 cctggcgatc agtgtccggc agttgccaaa gcgatcgatc gagacgaccg gcgcatggat
      781 ccggctgggc gtgtcctgga tcggtgccca cggtagcgcc acgagagagt cggtcggagc
      841 cggtgtaccg agctcggcca gcgcgacact cccggtcgag agatgcccgg caacgggggc
      901 gaagatatcg cgtccgtgga acgtcgacga gacgtccgcc aggaagtact cgggccggtc
      961 gaggacgacg acctcgcccg ggacgacatt ggccgtcagc agcgtcagca cgccgttgtc
     1021 cggcgtgacg aagaactgac cgcccgcctc gacaacgacc gctcggcgct caccgccaac
     1081 acctgggtcg acgacgatga cgtggatcgt gcctggagga aaggctgacc agaccgttgc
     1141 aatctggaag gccgcttcgg tgatgtcctg tggcgggata tggtgcgtca catcgacgat
     1201 cgtcacgttc cggttgaccc caagtaacga tcccttcatg gcggcgacgt agccgtcgac
     1261 cgtcccgaag tccgtcatca gcgtcacaat cccattggcg gcggccatcg gcttcgtcct
     1321 ccccaaacgt cacaccccgg ctggcaccga ctattgtcgc cgtatccagc cctcttgctc
     1381 ctgcatgtca tagacggcat cgagcagatc cgaccatgcc agcggatctt ccggaaaatg
     1441 cgtgatcaaa tgctcctcga aggcggccgt cgtcttccac cactcacggt cgtccatgtc
     1501 gccggccacc tcttcagcga gttctgccca gctgacctcg cgtccttcgg ccagcgcctg
     1561 ctgttccagc tccttcagat cccagagcca gtagccttct tcggcgacga cctcgatcac
     1621 atcctttcgg ctgcgccagc gcttgaccat ctcgacatag acagcccgcg ggatgagctt
     1681 gacgttcagt cgtggccctt cgtcggagtc gcggatgaac ttcgaataat cgcgctcggt
     1741 cactcctgat cctccacggt cgatacctgt tcctccagcc gccttctcgc agccgcccat
     1801 tcctctgggc ggttcgcatt gacaaacgac agcagttccg ggtcgtgctg gcggagccat
     1861 cgttcgggca attcccggac ccgcaaacca gccagcgcat cggcaacctt tagttcgctc
     1921 tgcgcgagtc gctgcttcag gtgcggcaga catacccgcc ggtagatcgc gtgcagtgtc
     1981 tcataggttc gcattcctcc ctggacatcg gcctgctggc cccgaaccgg caccagcacg
     2041 tcatagtcac gccgctcgtt cgccatcgcc tccagcaacg ccaccgacag gaacggcatg
     2101 tcgcaggcga cgaccagcac atgctcatac cgtgcctgcc ggagcgcggt cgcgatccct
     2161 cccaacgggc cgctacccgg ataggcgtcc gccacgaccg gaacgccgaa cttacggtat
     2221 ggctgacggt caccgacgat gaagatatcg ccggcgacgc gctcaacgga ccggattgcg
     2281 cgctccaaca gggtcgtacc tgcgacggag agcagcgcct tgtcactacc catccgacgg
     2341 cttgcgccgc ccgtcagaat cgcactactc aactcggcgc gggtcgtctg atgtgtcaat
     2401 gttgtgctca cgggccaccg cccgggccga ttgtaccgga gtttgacatg tccatcggct
     2461 gctgttccct gccgaagcaa tctgctacac tgagtggcat caaccgcgtc gcgcgctgga
     2521 aggtggcacg agatgagcgg cgatccaaaa gtcgagatgg tgactgcctg agcacccggc
     2581 taacgccgtg gtgctttttg ttatccatca cctcgccgga ccccgccagc ccgcagccgc
     2641 ttccgagccg gacgccaatc cgcggagcgc gggttgcaca gatgacggcg agaggagagc
     2701 tgccagtgaa ctccacgatg atcagcaaga tcgaaaaggc tcgtcgctat gccgaagagc
     2761 cggaacgtgt gaagttcgag cggttcgagg ttcgtttcca gggtacccac gacgaataca
     2821 ccgtcaaaat ggatggatcc gagtttacct gtacctgcca cttcttcgag gttcagcaga
     2881 tgggcacctg ctgccacatc atggcgctgc agcgcatgtt cgagacgatg atgaccgaag
     2941 aacagcacac cgccggagcg ccattcagct tcgcgacggc gtgacgacag gccgacgacc
     3001 tacacaagac agagctggat gacgacgagc gtcagttcaa ccggaacgcc ggttgggctg
     3061 gcgcgcgttc cgtctcatgc ggcatcgtca cgatgctgac gcggcccgcc tggacgatca
     3121 tctcccgtcg atcaaatccg gatggaccgg tcatgtccga cgcgcgcacg actggcgtgc
     3181 gcggctcggg cgcctcgaac ggcagcagca gcatcgccgc gcccgcgctc agccggagcg
     3241 ccgtatagta ctggacgaga caacgtgaca cccgcgagcg catctgatcg agtacctgtt
     3301 cttcagtgat tcgccggcat gccagcctgc aactctctcc gaccggccat ctataaagca
     3361 tactcgacgg catcgcggtt tccgacggct ggccgggagg catgacgtga gcacagcgca
     3421 ccggggacga aacctgtccg atctgacctg gatcgaagcg agcgaggtac tggtccgccg
     3481 cccgattggc ctgctgccga ttggagcgat cgaggcacac ggcccgcacc tgccactcga
     3541 cacggacgtc atcatcgcga ccgcgacagc ctcgcgagcg ctggaccggc tcgaagcggc
     3601 tggaactccg gcactgatcc tgccgccgat cacctactcc gtctcgttcg tcggcacctc
     3661 gttcgccggg acgacaccgg tcgagccgga cccgttcgag gcctacctga ccagcatcct
     3721 catccagcac ctgcgccagg gctaccgggc gatcgtctgc tgtaacgcgc acctcgaacc
     3781 ggcgagtgtc gagcgcgtcg cgactgccag ccgctccgtg acccagcgga ccggcacgcc
     3841 ggtcctgttt ccggaccagc gccttgaacc ctacgtcaat cttctcagcg acgagttccg
     3901 ggcaggtgcc cgccacgccg gtggctacga aacgtcgatc gtcatggcta cccggcccga
     3961 tgcggtccgg atggaccgtc tcgccggtct ggaaccggtc tggatcgacc tcccggcccg
     4021 cctgaaagag ggagcacaga cgttcgccga ggccggcgca acactcggct acttcggcga
     4081 accggcgaca tccacgatcg aggaaggcga gcgcatgctg gacgcgctgg cggagatcgt
     4141 catgatcgcc ctgcgcgacg ccggcgtcga tctgtcccct gacagtcgag ccaacgtgca
     4201 ccccctgtcg tcctgagccg cagcgaaggg cctctcggtg ttagccgagc atggcgtcaa
     4261 cgcagggggt gcacgttggc tcgactattc gattgaaggt tcgacgtagc tggcgagagc
     4321 ggtgcgccag tagtcaagcg tgtcgcgtag cgattgatcg gtcgaataga ggggctccca
     4381 gccggttgcg gagcgcagct tgccggcgtc tccggccacg atcggtgtgt cggatggacg
     4441 cgttcgggcc gggtccagcc ggacatcgac ggcgatcgtc gcctgatcga tgagccgatc
     4501 aagcaggtcg ccgacgcgat gggcgacacc cgaagcgatg ttgtagacct cgccgggagt
     4561 gccgaactca atcagcatcc ggtaggcgcg aacgacgtcg cgaacgtcga ggaagtcgcg
     4621 ccgcgcgtcg agattaccgg tcagaacgac cggctcgatc cggcccagtt ccgcctccgc
     4681 gacctggcgg gcgaaggtcg ccagcacgaa gcggtcgctc tgtccaggcc cgaagtggtt
     4741 gaacgggcga acgcgaacga tccgcatccc gaacgagagc gcatactgca agccgagcat
     4801 gtcctgcgcg atcttcgaga cggcatacgg gttgaccggc cggaacggct ggtcctcagt
     4861 caacggcacg tcttccggcg cgatctggcc gtactcctcg ctcgatccaa cgatcaggat
     4921 cgtcgggtcg agtccggctg cccggcagcc ctcaagcaag ttgatctggg cagtgacgtt
     4981 gttggagatc gtgtctgacg gcgcggcgaa ggcctgcggc acgtagctct gcgcagccag
     5041 gtggaagatc acatcc
//
LOCUS       DASUGJ010000799         3453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_270841, whole genome shotgun sequence.
ACCESSION   DASUGJ010000799 DASUGJ010000000
VERSION     DASUGJ010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270841"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFV93_18075"
     CDS             complement(<1..520)
                     /locus_tag="VFV93_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167321.1"
                     /translation="MGDPIQGLDRLRSEVLEGRLSRRDVLRRGALLGLSAPVIAGLLA
                     ACGSDDDDDDDTPAATNTAASGGTEPTNTAASGAEETKAPEDEATEASSPEATESGGS
                     EPTATTAAEPVGERGGGGNLRLLYWQAPVIMNPHLANGTKDFHASRVCLEPLVDFDKD
                     INPILVLAEEWPS"
     gene            complement(1103..3019)
                     /locus_tag="VFV93_18080"
     CDS             complement(1103..3019)
                     /locus_tag="VFV93_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5167322.1"
                     /translation="MSDELKGLTRLREDVLLGTLNRRDVLKRGAMLGLSAPVIAGLLA
                     ACGSDDDDDDNGDTAEPTNTTASGGAESTEAADEGEGTATEASTEASDEGAATETEGT
                     GGDEPAGERGGGGHLRLLYWQAPVIMNPHLANGTKDFHASRVCLEPLVDFDKDINPIL
                     VLAEEWPSVDNGLLAEDGKSVTWKLRQGVKWHDGEDFNADDVIFTYNYVTDVDTATTT
                     IGRYLTIESVEKIDDYTVKINFLEPNPAWYDPFSGENGLILAEHVFKDFMGKESRNAP
                     ANLAPVGTGPFKVTEFRPGDVILYDIFMDYWDPGKPHLDSIELKGGGDVTSAARAVLQ
                     SDEADWAWNLQIEPNVLAEIEEGGLGVVDALPGGGTERIMINQANPREEVNGAFSEPT
                     TKHPIFSDVRARNALALAIQRDTIAEQLYGKGGVAASNQYDEPAKFTNPDITWEFDLD
                     KAKALLDEMGFEGGDILYQTSINSVRQKTQEIVKQDLAKLGFNVEIKSVDSAVYFSTD
                     QGSPDTSGKFYADLEMHTNGPTVYPATWFERYRTDEIAEQANGWSGSNYFRYSNPEFD
                     KMHDQVRVEMDEDTQVQLFQDMLELVTVIDYVEVPVVNRTNLAAKSNKIQGYVGSPWS
                     SNPVYELKNWTMSE"
BASE COUNT          572 a         1116 c         1081 g          684 t
ORIGIN      
        1 gcgacggcca ttcctctgcc aggacgagga tcgggttgat gtccttgtcg aagtcgacca
       61 gcggctccag gcagacgcgc gaggcgtgga agtccttggt gccgttggcc aggtgcgggt
      121 tcatgatgac cggcgcctgc cagtagagca gccgcaggtt gccgccgcca ccgcgctcgc
      181 cgaccggctc ggccgccgtc gttgcggtcg gctctgagcc acctgactcc gtcgcctcgg
      241 gggacgacgc ctcggtggcc tcgtcttctg gtgctttggt ctcctcggca ccggaagcag
      301 ccgtgttggt tggctcagtg ccaccactgg cagccgtgtt cgttgccgcc ggagtgtcgt
      361 cgtcgtcatc gtcgtccgag ccgcaggccg ccagcaggcc ggcgataacc ggcgcgctca
      421 ggcccagcag cgcaccgcgc ctgaggacat cgcggcgact cagtcgccct tcgagtacct
      481 cggaccgaag tcgatcgagt ccctgaatgg gatcgcccaa accggatctc ctttccctca
      541 accatccgcg gcgcagaacg actccgccac cgctcgaccg cgtccgggac catcccgatt
      601 cgcggtgccc gtgtccagcc ggtacaggaa gtcgtcacgg caactgctgc cgttagacgc
      661 aatactccag gccaacacga cacgggcgcg aagggtaccg gcctgttcgc aacggactcg
      721 acgccgcccc cggcgtcggc ccatcttcga acgttgtccg cgagggaacc ccacatgtgc
      781 ctcgtccgga cagccgatcg atggaagcgt ctgcgatcgg atcgtatgtt gatgcacgga
      841 tgccgcgcat ctccaccgat gttcgctcca catcgtcctc gcttcccata ttaacgcacg
      901 aatgccgcga actggatgcc tcccgcgttt gcaatgctcg atttgggaag tgttctgccg
      961 cgtggtgcag atatgcgcat atgcggtaac ggtgatcgtg atccggctgt cgcaagtccg
     1021 atccggagac aggacacaac aacgccggcc gcgacatctg tcgcggccgg cgtctgcgct
     1081 gaggcgctgt ggggcggagt tgttactccg acatcgtcca gttcttcagc tcgtagaccg
     1141 ggttggacga ccacggcgag ccgacgtacc cctggatctt gttggacttg gccgccaggt
     1201 tcgtgcggtt gacaaccgga acctcgacgt agtcgatgac cgtcaccagc tccagcatgt
     1261 cctggaacag ctgaacctgg gtgtcctcgt ccatctcgac gcgcacctgg tcgtgcatct
     1321 tgtcgaactc ggggttcgaa tagcggaagt agttggagcc ggaccagccg ttggcctgct
     1381 cggcgatctc atcggtccga tagcgctcga accaggtcgc cggataaacg gtcgggccat
     1441 tcgtgtgcat ctcaagatcg gcgtagaact tgcccgacgt gtcaggcgaa ccctgatcgg
     1501 tcgagaagta gacggccgag tcgacgctct tgatctcgac gttgaagccg agcttggcca
     1561 gatcctgctt gacgatctcc tgcgtcttct ggcggacgga gttgatcgag gtctggtaga
     1621 ggatgtcgcc accctcgaag cccatctcgt ccagcagggc cttggccttg tcgaggtcga
     1681 actcccaggt gatatcggga ttggtgaact tggccggctc gtcgtactgg ttcgaggcgg
     1741 cgacgccgcc tttgccgtag agctgctcgg cgatcgtgtc gcgctggatc gcgagcgcca
     1801 acgcgttgcg ggcgcgcacg tccgagaaga tggggtgctt ggtcgtcggc tccgagaagg
     1861 cgccgttgac ctcttcgcgg gggttggcct ggttgatcat gatgcgctcg gtgccgccac
     1921 ccggcagcgc gtcgacgacg cccaaaccgc cttcctcgat ctcggccagc acgttcggct
     1981 cgatctgcag gttccaggcc cagtcggcct cgtccgactg caagacggcc cgggctgcgc
     2041 tggtgacatc accgccaccc ttgagttcga tcgagtccag atgcggctta cccggatccc
     2101 agtagtccat gaagatgtcg tagaggatca cgtcgccggg gcggaactcg gtgactttga
     2161 acgggccagt tccgaccggc gccaggttgg ccggcgcgtt gcgcgactcc ttgcccatga
     2221 agtccttgaa gacgtgctcg gcaaggatca ggccgttctc accggagaac ggatcgtacc
     2281 aggctgggtt cggctccagg aagttgatct tgaccgtgta gtcgtcgatc ttctcgaccg
     2341 actcgatcgt caggtaacgc ccgatcgtcg tcgtcgccgt atcgacgtca gttacatagt
     2401 tgtaggtgaa gatgacatcg tcggcgttga agtcctcgcc gtcatgccac ttgacgccct
     2461 ggcgcagctt ccaggtgact gacttgccat cctcagccag cagaccgttg tcaaccgagg
     2521 gccattcttc ggcaaggacg aggatcgggt tgatgtcctt gtcgaagtcg accagtggct
     2581 ccaggcagac gcgggacgcg tggaagtcct tggtgccgtt ggcgagatgc gggttcatga
     2641 taaccggcgc ctgccagtag agcagccgca ggtggccgcc gccaccgcgc tcacctgccg
     2701 gctcatcgcc gccggtgcct tcggtctcgg tcgccgcgcc ctcatcagag gcttcggtag
     2761 aggcttcggt cgcggtgcct tcgccctcgt ccgccgcctc ggtgctctcg gctccgccgc
     2821 tggcagtcgt gttggttggc tcggcggtgt cgccgttgtc atcgtcatca tcgtcggagc
     2881 cgcaggccgc cagcagtccg gcgatcaccg gcgcgctcag gccgagcatc gcgccccgct
     2941 tgagcacatc acggcggttc agcgtcccca gcaggacatc ctcacgcagc cgtgtcaaac
     3001 ccttcagttc gtcactcact cgttggggtt cctttcctcc accgaaacag gttgcggcct
     3061 cgctgccgcc gtcaccgacg atcgctgctg acgctcgtcg ctgcgcatca atggaggaat
     3121 cgacgccgaa ccgcgcgcca tcggccccgg agtcggctgc gaacaggatg cagctaaccg
     3181 gtagacgctg gtcacgcgct cagaacttcc gaaacctgcc gacccatgca caggagcagc
     3241 ggtcggacag acgcaaacac tcccaaaccg gcggtgatgt ttggcccatc atatgcgtat
     3301 acgcgcaagt cgctattgcc ggatgtatct ggagtcacgg gatttctatt tgccataacg
     3361 acgcagaggg acttcttgtt gcgatatact ggctccacgc catcacttta gattgtgcac
     3421 cacgatggac gtatcgcgta ggggcgatgg ccg
//
LOCUS       DASUGJ010000800         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1936092, whole genome shotgun sequence.
ACCESSION   DASUGJ010000800 DASUGJ010000000
VERSION     DASUGJ010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936092"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VFV93_18085"
     CDS             complement(<1..808)
                     /locus_tag="VFV93_18085"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5167323.1"
                     /translation="MAEPTIRIRHQSRVRPVESGTYHVLLRVMAPKVMRQERTRPPLD
                     LAFVVDRSGSMSGGAFVLARQGVEHALRLLDERDSVSVVVYDDRIDTLLTQRLLERDA
                     RDKAVRRLRRVGPRGSTDLAGGWLTGCDQLAPIADGSRPLRRDDARSIVRTLLLTDGL
                     ANVGMTNHDEIAMHAGELARRGISTSTFGTGAGYDEDLLARMADAGRGHFHHIPNAAA
                     IPQVFAGELGEMLELALRDVTLSLRFPAGWQGSLMNDLPLERHGDWIRLSL"
     gene            complement(955..1665)
                     /locus_tag="VFV93_18090"
     CDS             complement(955..1665)
                     /locus_tag="VFV93_18090"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5167324.1"
                     /translation="MSVAGWSVRELAEQAGTTVRTIHYYISEGLLPPPAGATRSATYS
                     EAHLARLRLIGALRDEGLALASIRQRLAPLSDDQALEVVADLDEHLADPEATPLTTLG
                     LIEAALASRTLDDTPPIASPMEVRPAMTTSYQSIEPTRPDSASDYLKRVLRKPERRPE
                     QPVPMPRPMPRPAPPPRSQLDTNRPETWHHYQIEDGIELRVREDRYRESKGRLLAVID
                     TLKTSLRRFGLTDHRDDV"
     gene            1923..>2360
                     /locus_tag="VFV93_18095"
     CDS             1923..>2360
                     /locus_tag="VFV93_18095"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5167325.1"
                     /translation="MERELDDATAGVGAVDVTGSRIAPVLTRWRHGFGWTQEELATRA
                     GISRGYLSRLERGLPGRPGLDILSRVCGALGRTWTELFSEAGLSLPGDVTLADIGDGL
                     HDPELILYLRRLPELDGRDRAVLRAVLRAFFEREAADRAALPAS"
BASE COUNT          358 a          837 c          762 g          403 t
ORIGIN      
        1 ccagcgacaa gcggatccag tcgccatgtc gctccagcgg caggtcgttc atcagcgatc
       61 cctgccagcc ggccggaaac cgcagcgaga gcgtgacgtc gcgcagcgcc agctccagca
      121 tctcgccgag ctcaccggca aacacctgcg ggatcgctgc cgcgttcggg atgtggtgga
      181 agtgaccccg gccggcgtcc gccatccgcg ccagcaggtc ttcgtcgtac cccgcgccgg
      241 tgccgaaggt cgaggtgctg atgccacgtc gtgccagctc gccggcgtgc atcgcgatct
      301 catcgtggtt ggtcatcccg acgttggcga gcccgtccgt cagcagcagg gttcggacga
      361 tcgagcgggc gtcgtcgcgt cgcaggggcc ggctgccatc cgcgatcggc gcgagctgat
      421 cgcagccggt cagccagccg ccggccaggt cggtcgagcc gcgcggcccg actcgccgca
      481 gccgccgaac ggccttgtcg cgcgcgtcgc gttccagcaa ccgctgcgtg agaagcgtgt
      541 cgatccggtc gtcgtagacc acgaccgaga cgctgtcgcg ctcgtccagc agccgcagcg
      601 cgtgttcgac gccctgccgc gccagaacga acgccccacc cgacatcgag ccggaacggt
      661 cgacgacgaa cgccagatcc agcggcggcc gggtccgttc ctggcgcatg accttcggcg
      721 ccatcacccg cagcaggaca tggtacgtac cggactcaac cggccgaacg cgcgactgat
      781 gccggattcg aatcgttggt tcagccatcg cgggactccc ttcaaccgtc gccgctacag
      841 aggcgacatc acccaggccg cgcgccaccg cgcgacatga cgatcagcac atgttcgatg
      901 tcctgtgagc gctttggaaa cggggaccga tttcagttcc ccgtgtatcg gcccctacac
      961 gtcgtcgcga tggtccgtca gcccgaatcg gcgtagcgag gttttcaatg tgtcgatcac
     1021 cgccagcaac cggcccttcg actcccggta gcgatcctcg cgcacgcgca gctcgatccc
     1081 gtcttcaatc tgatagtggt gccaggtctc cggccggttc gtgtcaagct gtgaccgtgg
     1141 cggcggggct ggacgcggca tcggcctcgg cattgggacc ggctgttccg gccggcgttc
     1201 gggcttgcgc aacacccgct tcagatagtc gctggccgaa tcgggccggg ttggctcgat
     1261 agattggtac gaggttgtca tcgcaggccg cacttccatt gggctggcga tcggtggcgt
     1321 gtcatccagt gtccggctgg ccagcgccgc ctcgatcagc ccgagcgtcg tcaacggcgt
     1381 cgcctccggg tcagccagat gctcgtcaag gtcggcgacg acctccagcg cctgatcgtc
     1441 cgagagcggc gccagccgct gcctgatact ggccagcgcc agaccctcgt cccgcagcgc
     1501 gccgatcaac cgcaagcgcg ccagatgcgc ctctgaatag gtcgcactcc gcgttgctcc
     1561 ggccggcggc ggcaacagtc cctcactaat gtagtagtgg atcgtccgca cggtcgtccc
     1621 ggcctgctcg gccagttcgc gaacactcca gccagctacc gacatacgct cttccatcaa
     1681 tcgctccaag agtacgatcg agataatagc agtatcacgc actactgtca ataatgttga
     1741 gcggctagct cacgctcgtt cgccatcgct tggcgcgcag cttacgctcg tacgccgttc
     1801 ggccggggag cgtaagctgc caggccgccc ccatcacgcg tcacgcatca cgcgtcacgc
     1861 gtcacgccgc agcctgcggt atcctctcca gcatcgcttc gacgtacgtt gggagggctg
     1921 ccgtggagcg tgaactggac gatgctacgg ccggtgtcgg cgccgttgat gtcaccggca
     1981 gccggatcgc gccggtgctg acgcgctggc ggcatggctt cggctggacc caggaggaac
     2041 tggccacgcg cgccggtatc tcgcgcggct acctctcgcg gctcgaacgg gggctgccgg
     2101 gccggcccgg tctcgacatc ctctcgcggg tctgtggcgc gctcggccgg acctggacgg
     2161 agctgttcag cgaagcaggt ctctcgctgc ccggcgatgt cacgctggcc gacatcggcg
     2221 acgggctcca cgatcctgag ttgattctct acctgcgtcg cctgccggag ctcgacggcc
     2281 gcgatcgcgc cgttttgcgg gccgtcctgc gtgccttttt cgagcgcgag gccgccgacc
     2341 gcgccgcgct gccggccagc
//
LOCUS       DASUGJ010000801         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_965045, whole genome shotgun sequence.
ACCESSION   DASUGJ010000801 DASUGJ010000000
VERSION     DASUGJ010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965045"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..168)
                     /locus_tag="VFV93_18100"
     CDS             complement(31..168)
                     /locus_tag="VFV93_18100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HEX5167326.1"
                     /translation="MDDNLGYLFAAFGVTWLAIAGYLLYIGQQVKGLRDEVRLLDEGD
                     E"
     gene            complement(174..953)
                     /gene="ccsA"
                     /locus_tag="VFV93_18105"
     CDS             complement(174..953)
                     /gene="ccsA"
                     /locus_tag="VFV93_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5167327.1"
                     /translation="MIQSKSTNRSDLLVTGLGLLAVAVMTVGIFMAFVYAPTDADQGD
                     AQRIFYVHVPMAWLAYLAMGVVLVGSVGYMWKRSDRMDRLARSSAELGFLFTTLVLIT
                     GSLWGRPIWNAWWQWEARLTTTLILWFIFIGYFMVRAYAGDRERGARYAAVLGIIGAI
                     DVPIIHQSVKWWRTLHPEPIVLDSSGPNLPDSMLMTLLVCLVGFTLLYAFMMVLKMRI
                     EWARDVLAERELTALLAPEPEPRDAGQMAPVSRNVSGAAED"
     gene            complement(968..1627)
                     /locus_tag="VFV93_18110"
     CDS             complement(968..1627)
                     /locus_tag="VFV93_18110"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5167328.1"
                     /translation="MRRVWALVWKDLLVEFRGREFLGAMVIFALITIVTLNFAFDLNR
                     GEREAPGAGGLWIAFLFAGMLGLGRSLAVERDRGTLEGLTLCPVDAGTIFLGKFVSNV
                     LFIVIVQAVSLPVFYGLYNLPALRPMVWLIALTGAIGFAGLGTLFSAVAASSRAREIL
                     MPLLLFPLAIPLVIGTVRATTLIFQGEGGDAWPWFNLLAAFDVIFVTLSYVVFDLVLE
                     E"
     gene            complement(1634..2356)
                     /gene="ccmA"
                     /locus_tag="VFV93_18115"
     CDS             complement(1634..2356)
                     /gene="ccmA"
                     /locus_tag="VFV93_18115"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX5167329.1"
                     /translation="MTGEVPLFQVEHVYKRFGTKVALRDVTFRLATGEAVALLGANGA
                     GKTTLLRTLATLARPTRGRVSAFGVDAWSARPDVRARIGVVAHQPYVYPELSCAENLI
                     FFATMFNLDAASDRAAAALARVGLARRAEDRAATLSRGLLQRLNLARAILHEPAALVL
                     DEPDTGLDAAGREVLSEIVQAQVAGGGSVVLTTHALEYALELATRVVVLAGGRVVLDA
                     PRDEVSGAAVTQAIGGVVAAGR"
     gene            complement(2353..2607)
                     /locus_tag="VFV93_18120"
     CDS             complement(2353..2607)
                     /locus_tag="VFV93_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167330.1"
                     /translation="MTLALVALAVLAVVVFAFVLEPVVRARGDRTVIDTVALPEHEAP
                     LEDVEALEPDADEPTAAIDGDERLSGRRLPIDRPAGSDAS"
BASE COUNT          516 a          968 c          772 g          374 t
ORIGIN      
        1 ccctgccgga ttgtgggatt ggattattgg ttattcgtcg ccctcgtcga ggagccgcac
       61 ctcgtcgcgc agccccttga cctgctggcc gatgtagagc aggtagccgg cgatcgccag
      121 ccaggtgacg ccgaaggccg cgaacagata gccgagattg tcgtccatga ttcctaatcc
      181 tccgccgccc cggatacgtt gcgagacacc ggagccatct ggccggcgtc gcgcggctca
      241 ggttccggcg cgagcaacgc cgtcaactcc cgttcggcca gcacgtcgcg cgcccattcg
      301 atgcgcatct tcaacaccat catgaaggcg tacagcagcg tgaacccgac caggcagacc
      361 agcaaggtca tcagcatcga gtccggcagg ttcggcccgc tcgaatccag cacgatcggt
      421 tccggatgca acgtccgcca ccacttgacc gattggtgga tgatcggcac gtcgatcgcg
      481 ccgatgatcc ccaggacagc cgcgtagcgc gcgccccgct cgcgatctcc cgcgtaggcg
      541 cggaccatga agtagccgat gaagatgaac cagaggatca gcgtcgtcgt cagccgcgct
      601 tcccattgcc accatgcgtt ccagatcggc cgcccccaga gcgagccggt aatcagcacc
      661 agcgtcgtga acaggaagcc caattctgcc gacgagcgcg ccagccggtc catccgatcc
      721 gagcgcttcc acatgtagcc aacgctgccg accagaacga cgcccatcgc caggtacgcc
      781 agccacgcca tcggcacgtg cacgtagaag atgcgctgcg cgtccccctg atcggcatcg
      841 gtcggcgcgt agacgaacgc catgaagatg ccaaccgtca tgaccgctac cgcaagcagg
      901 ccgaggcccg tcacgagcag gtcagaccga ttggtcgatt tcgactgtat catctgttgg
      961 ttccggatca ttcttcgagg accaggtcaa acacgacgta ggacaatgtg acgaaaatca
     1021 catcgaacgc cgcaagcaga ttaaaccacg gccaggcatc cccgccctca ccctggaaga
     1081 tcagcgttgt tgcccgcacc gtcccgatca cgagcgggat cgccagcggg aagagcagca
     1141 gtggcatcag gatctcacgc gcccggctgc tggcggcgac agccgagaag agcgttccca
     1201 gcccggcgaa gccgatcgcg cccgtcagcg cgatcagcca gaccatcggg cgaagcgccg
     1261 gcaggttgta caatccgtag aacaccggca gcgagacggc ctgaacgatg acgataaaga
     1321 gcacgttcga gacgaacttg ccgaggaaga tcgtcccggc atcgaccggg cagagcgtca
     1381 gcccctccaa cgtcccgcgg tcccgctcga cggccagcga gcgccccaac cccagcatcc
     1441 cggcgaacag gaacgcgatc cagagccctc ccgcccccgg cgcttcccgc tcgccccgat
     1501 tgagatcgaa ggcgaagtta agcgtcacaa tcgtgatcag cgcgaagatg accatcgcac
     1561 cgaggaactc gcgcccccgg aactcgacca gcagatcttt ccagaccagc gcccagaccc
     1621 gccgcatcgc ccgctaccgt cccgccgcaa cgacgccgcc gatcgcctga gtgacggcgg
     1681 ccccgctgac ctcgtcacgc ggcgcatcca gcacaacccg cccgccggcc agcacgacca
     1741 cccgcgtcgc cagctccagc gcgtactcca gcgcgtgcgt tgtcaggacg acgctgccgc
     1801 caccagccac ctgagcctgg acgatttccg atagtacctc gcgaccagcc gcgtccagcc
     1861 cggtgtccgg ttcatccagc accagcgccg ctggttcgtg caggatcgcc cgggccaggt
     1921 tcagccgctg caacagcccg cgcgagaggg tcgccgcccg gtcctcggcg cgccgtgcca
     1981 gcccgaccct cgccagcgcc gctgccgccc gatcgctcgc ggcatcgaga ttgaacatcg
     2041 tcgcgaagaa gatcaggttc tcagcgcacg agagttccgg gtagacgtag ggctgatgcg
     2101 caacgacgcc aatccgcgcg cgaacatccg gccgcgcgct ccaggcatcg acgccgaatg
     2161 ccgaaacacg cccgcgcgtt ggccgcgcga gtgtcgccag cgtccgtaat aaggtcgtct
     2221 tgccggcgcc gttggcaccc agcagggcga ccgcctcgcc ggtagccagg cgaaacgtga
     2281 catctcgaag ggcgaccttc gtgccaaacc gcttgtagac gtgctccacc tgaaaaagag
     2341 gaacctcgcc ggtcacgacg cgtcgctgcc ggccggccgg tcaatcggca gccgccgtcc
     2401 gctcagccgc tcgtcaccgt cgatcgctgc cgtcggctcg tctgcgtccg gctccaatgc
     2461 ctcgacgtct tccaacggcg cttcatgttc gggcagcgcg acggtgtcga tcaccgtccg
     2521 atccccccgc gcccggacaa ccggctcgag cacgaacgcg aaaacgacga cggccagcac
     2581 cgccagagcg accagcgcca gcgtcacgct cctgacatcc tctcagcgcc
//
LOCUS       DASUGJ010000802         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_408603, whole genome shotgun sequence.
ACCESSION   DASUGJ010000802 DASUGJ010000000
VERSION     DASUGJ010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408603"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..2541)
                     /locus_tag="VFV93_18125"
     CDS             complement(187..2541)
                     /locus_tag="VFV93_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167331.1"
                     /translation="MTTISDHDIRVALTDASLDAPRVDLWERLEAPVAGLSRTRHPLR
                     RGLRALAAMLAILLAGAGMLTIFSLADDAPDQRARQIDAVPDLLLMTTWDDQTQSSNL
                     RAYLPESSDVVNILDDVSVSDRPVISHDGQQIVYTGWQREGETGTLRLWSLDSATLAM
                     QWSVVITSIPVAADGTSTNAGYSTAIAGDRVYVSWNAWDTPFPVPIVAIDRATGEQVG
                     RWEITADAAAGETPLHVGSSWLTASPDGSQLDVLMQVWDVDLPSSNQVRLAYVAFDLP
                     EMRETQRVVQAPDSSPEERFYPRDPGRTPDGKTLYAVNGGYYDDPLRVEFFDLDSGTL
                     TDKIELGFGTGFEISQQTAVSADGRLLYIFDSTTGKLATVDLVKRRLVGTATVDMSIV
                     QGSRGSLLGRAWDAVKRVFVDDAAAKIPFSGSMQLSPDGSRLYAIGISGRDEVPDGVL
                     VIDTSSWQVVDHWLAGEHPIQLISGSGGRYLYALTISWANQGESGMRIIDTTSGEEVV
                     VNDELREIASTYGTYSVAEIYRDQWGVSPAIAGVDPDDLAVPKHVDPYASMSVRVSTN
                     ATMAGDPVTVELRYLDPKSDEPISEDTDSVRYDEPQHVRALMSKNNSNTTEQTIVLAR
                     SGYGVYRGAAVLPSPGTWTVQVIAEREGEPSRYASVRDAVVVQPTQLGSDGRRYKLAV
                     ELVEPPAHVEQESPVRVSIVDAETGAPLPEDVELAGGMPETMAGSATLEARAVTSADL
                     HPTGHGVYEGTFTFFASGSWSIAVNFPQDGLRSGGVAVGVVEVE"
BASE COUNT          405 a          869 c          959 g          472 t
ORIGIN      
        1 ttcccccagg acggcctgcg gtcaggtggc gttgcggtgg gggtggtgga ggtggagtga
       61 cggccctcac cccccaaccc cctctcccaa ttctgggtga gggggagcct gtggcgggcg
      121 tacggctgcc ggacttacac tccccctctc ccagaattgg gagagggggt tggggggtga
      181 gggccgtcac tccacctcca ccacccccac cgcaacgcca cctgaccgca ggccgtcctg
      241 ggggaagttg acggcgatgc tccagctgcc ggaggcgaag aaggtgaagg taccttcgta
      301 gacgccgtgg ccggttgggt gcaggtcggc cgaggtgacg gcgcgggctt cgagggttgc
      361 cgagccggcc attgtttccg gcatcccgcc ggccagttcg acatcctcgg gcagcggcgc
      421 gccggtttcg gcgtcgacga tgctgacgcg gaccggtgac tcctgctcga cgtgcgcggg
      481 tggctcgacc agctcgacgg ccagcttgta gcggcgaccg tcgctgccaa gctgggttgg
      541 ctggacgacg acggcgtcgc ggacgctggc gtagcggctc ggctcgccct cgcgctcggc
      601 gatgacctgg accgtccagg tgcccggcga tggcaggacg gccgcgccac ggtagacgcc
      661 gtacccggag cgggccagca cgatcgtctg ctcggtcgtg ttcgagttgt tcttcgacat
      721 cagcgcgcgg acatgctgtg gctcgtcgta gcggacgctg tcggtgtcct cgctgatcgg
      781 ctcgtcggat ttcgggtcga ggtagcgcag ctcgaccgtg acggggtcgc cggccatcgt
      841 cgcgttcgtg ctgacgcgga ccgacatcga cgcgtagggg tcgacatgct tcgggacggc
      901 caggtcgtcg ggatcgacgc cggcgatggc ggggctgaca ccccactggt cgcggtagat
      961 ctcggcaacg gagtaggtgc cgtaggtgct ggcgatctca cgcagttcat cattgacgac
     1021 gacttcctcg ccgctggtcg tgtcgatgat gcgcatgccg gattcgccct ggttggccca
     1081 gctgatcgtc agcgcgtaga ggtagcgccc gccgctgccg gagatgagct ggatcggatg
     1141 ttcgccggcc agccagtgat cgaccacctg ccagcttgat gtgtcgatga cgaggacacc
     1201 atccggcacc tcatcgcgcc cgctgatgcc gatcgcgtag aggcggctgc catccggcga
     1261 gagttgcatc gagccgctga acggaatctt ggcggcggca tcgtcgacga agacacgctt
     1321 gacggcgtcc caggcccggc ccagcagcga gccgcgtgac ccctggacga tgctcatatc
     1381 gaccgtcgcc gtcccgacca gccgccgctt gacgaggtca acggtcgcca gcttgccggt
     1441 ggtggagtcg aagatgtaga gcaggcgacc atcggccgag acggccgttt gctgcgagat
     1501 ttcgaagccg gtcccgaagc ccagctcgat cttgtcagtc agcgtgccgc tgtcgaggtc
     1561 gaagaactcg acccgcagcg ggtcgtcgta gtagccgcca ttgacggcgt agagcgtctt
     1621 cccgtccggc gtgcgtccgg gatcgcgtgg gtagaagcgc tcttcgggac tgctgtcggg
     1681 cgcctgaacg acgcgttgtg tctcgcgcat ctccggcaga tcgaacgcga cgtaggccag
     1741 ccgaacctgg ttcgaggatg gcagatcgac atcccagacc tgcatcagaa catccagttg
     1801 gctaccgtcc ggcgaggctg tcagccagga cgagccaacg tgcagcgggg tctcaccggc
     1861 ggcggcgtcg gcggtgatct cccaacgccc gacctgctcg ccggtggcac ggtcgatggc
     1921 cacgatcgga accgggaacg gggtatccca ggcgttccac gacacgtaga cgcgatcacc
     1981 ggcgatcgcc gtgctgtagc cggcgttcgt ggacgtgccg tccgctgcga cggggatgga
     2041 ggtgataaca accgaccact gcatcgccag cgtcgcacta tcgagcgacc agagccgcag
     2101 tgtccccgtc tcaccttccc gctgccagcc ggtgtagacg atctgctgtc cgtcgtgcga
     2161 gataaccggc cggtcgctga cgctgacatc gtcgaggata ttcacgacat cgctggactc
     2221 cggaagatac gcgcgcaggt tactgctctg ggtctgatcg tcccaggtcg tcatcagcag
     2281 caggtcgggg acggcgtcga tctggcgggc gcgctggtcg ggggcgtcgt cagccagcga
     2341 gaagatcgtc agcatcccgg cgccggccag caggatcgcc agcatggccg ccagcgcgcg
     2401 cagcccgcgc cggagcgggt ggcgggtacg gctgaggccg gccaccggcg cttccagccg
     2461 ctcccagaga tcgacgcgcg gcgcgtcgag gctggcgtcg gtcagcgcaa cgcggatgtc
     2521 gtggtcgctg atagttgtca tcgtgatgcc ctccgttcgg gctgagtggt tgtcgtttcg
     2581 atctcgccgc cctgctcgtc acccagcagc ctccggagct gctcgcgtcc gcgatggagc
     2641 cgggatttga cggtgccggg ctggctgtcg gtcgcgctgg cgatctcgtc tgtcgagagg
     2701 tcgtt
//
LOCUS       DASUGJ010000803         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2077063, whole genome shotgun sequence.
ACCESSION   DASUGJ010000803 DASUGJ010000000
VERSION     DASUGJ010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077063"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..927)
                     /locus_tag="VFV93_18130"
     CDS             complement(67..927)
                     /locus_tag="VFV93_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX5167332.1"
                     /translation="MADATPERVTIYEVGPRDGLQNEATPIPTEAKIRFVHALAGAGL
                     HWIETTSFVNPKAVPQLADATEVMQGIERSAGVRYPVLVPNERGMERALEANVDAIAL
                     FAAASEAFSQANIRVSIDESFERFKPVAEIAKRDKIWTRGYVSTAFHCPYSGPVAPAQ
                     AIEVAERLFALGCDEVAIADTIGRATEDDTSRLLDLATSRLPIARLAFHLHDTTGLAL
                     ANIRLALAAGITVFDGSAGGLGGCPFAPGAPGNLSTEKLVRMLDEMGIETGVNADAVA
                     QAALEMRALL"
     gene            complement(920..>2495)
                     /locus_tag="VFV93_18135"
     CDS             complement(920..>2495)
                     /locus_tag="VFV93_18135"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5167333.1"
                     /translation="VLCGDKIATREAAERANVPTLPASQPLTLLDESQWADAAEWVGY
                     PLIAKVASGGGGRGLRVAESAGAVEGAIRSALREAGASAAGTTLYLERYLEGARHVEV
                     QVAGDATDAVALGDRDCSIQRRHQKVVEEAPAFGLSADLRRELHSHAVAMAREVGLLG
                     VGTIEFLLSRDHELFFIEINPRLQVEHTVTEEVTGMDLVELQLQLAEGGRLPEPVEPQ
                     GHAIQARLYAEDPLHDFAPSPGEIRLLDWPSQPGLRIDAGYESGDVVPSFYDSMIGKL
                     IVHAHDREAAITALADACNNLLIGGIATNRAWLLALTQLDAFRSTTHSLATAARVEVP
                     RRPVSALSQLAVSLLPDDPGGSAWATVGPFRIVAPACLALHDPDGRWEGRVIYASQES
                     GWAPILEDDADATDLPAVVIDQGEGFEVSDPTGRWLVMLGPLPRQQAGAETADGSLRA
                     PMPGTVVAVNVTPGQRVTKGEVLAVMTAMKMEMTLAAPFDGIVTGVGGAPGDLVSSRQ
                     VLVTVAKDAESEANDG"
BASE COUNT          376 a          845 c          864 g          410 t
ORIGIN      
        1 gacgcgaccc agacggacgg ttcttcacac gcgccgtgcg tccgggccag ttcgctgaat
       61 ggcggcctac agcagggccc gcatctccag cgccgcctgc gcgacggcgt cggcgttgac
      121 gccggtctcg atgcccatct cgtccagcat cctgacgagc ttctcggtgg acaggttgcc
      181 gggggcgccg ggcgcgaacg ggcagccgcc gaggccgccg gcggagccat cgaagacagt
      241 gatgccggcc gcgagcgcca gccggatgtt ggccagtgcc agcccggtcg tgtcgtgcag
      301 gtggaaggcc agtctcgcga tcggcaggcg gctggtagcg agatccagca gccggctggt
      361 gtcatcttcc gtcgcgcggc cgatcgtgtc ggcgatggcg acctcgtcgc aaccgagcgc
      421 gaagagccgc tcggcgacct cgatggcctg ggcgggcgca accggtccgg aataggggca
      481 atggaacgcg gtcgagacgt agccgcgcgt ccagatcttg tcgcgcttcg cgatctcggc
      541 gaccggcttg aagcgttcga acgactcgtc gatcgatacc cggatgttgg cctgcgagaa
      601 ggcctccgag gctgccgcaa agagggcgat cgcgtcgacg ttcgcctcca gcgcccgctc
      661 catcccgcgc tcgttcggaa cgaggacggg gtagcgcacg cctgcgctgc gttcgatgcc
      721 ctgcatgact tcggtggcgt cggccagttg cgggacggcc ttcgggttga cgaacgaggt
      781 cgtctcgatc cagtgcaggc cggcaccagc cagtgcgtgg acgaagcgga tcttggcctc
      841 ggtcgggatc ggcgttgcct cgttctggag gccgtcgcgt gggccgacct cgtagatcgt
      901 gacgcgttct ggcgtcgcat cagccatcgt tcgcctcgct ttcggcgtcc ttcgcgaccg
      961 tcaccagcac ctgccgcgaa ctgaccaggt cgccgggagc gccgccgaca ccggtcacga
     1021 tgccgtcgaa cggggcggcc agcgtcatct ccatcttcat cgccgtcatg acagccagta
     1081 cctcgccttt cgtgacacgc tggcctggcg tgacgttgac ggccacgacc gtgcccggca
     1141 tcggcgcccg caacgacccg tcggctgttt ccgcgccggc ctgctgtcgc ggtagtggac
     1201 cgagcatgac cagccagcgc ccggtcgggt cggagacctc gaacccctcg ccctggtcga
     1261 tgacgacagc cgggagatcg gtcgcgtcgg cgtcgtcttc caggatcgga gcccagcccg
     1321 attcctggga agcatagatg acgcggcctt cccatctgcc gtccggatcg tgcagtgcca
     1381 ggcacgccgg cgcgacgatt cggaacggcc cgaccgtcgc ccaggccgag ccgccgggat
     1441 cgtccggcag gagcgatacg gccaactggc tcagcgccga tacgggacgg cgaggcacct
     1501 cgaccctagc ggcggtcgcg agcgaatgag tcgtgctccg aaacgcgtcg agctgggtca
     1561 gggccagcaa ccacgcgcga ttggtcgcga tcccgccaat cagcaggttg ttgcaggcgt
     1621 ccgccagtgc ggtgatggcg gcttctcggt cgtgagcgtg gacgatcagc ttgccgatca
     1681 tcgagtcgta gaagctgggg acgacatcac cggactcata gccggcgtcg atgcggaggc
     1741 caggctggct tggccagtcg agcagtcgaa tctcgcctgg gcttggtgcg aagtcgtgga
     1801 gcgggtcctc ggcgtagagc cgggcctgga tcgcatgtcc ctgcggctca accggctccg
     1861 gcaagcggcc gccttcggca agctggagct ggagttcgac cagatccatg ccggtaactt
     1921 cttcggtgac ggtgtgctcg acctgcaggc gcgggttgat ctcgatgaag aacaactcat
     1981 ggtctcgcga gagcaggaat tcgatcgtcc cgacgccgag caggccgact tcgcgtgcca
     2041 tcgcgacggc gtggctgtgc agctcgcggc gcaggtcagc ggataggccg aaggccggtg
     2101 cttcctcgac gaccttttga tgccggcgct ggatcgagca gtcacggtct ccgagcgcga
     2161 cggcatcggt tgcgtcgccc gccacctgga cctcgacatg cctggcgccc tccaggtagc
     2221 gttcgagata gagcgtcgtc ccggccgccg acgccccggc ctcgcgcaga gcagatcgga
     2281 tggcaccttc gaccgccccg gcgctctccg cgacccgcag cccgcgcccg ccaccgccgg
     2341 acgcgacctt ggcgatcagg ggataaccga cccactcggc tgcgtctgcc cattgcgatt
     2401 cgtcgagtaa cgtcagcggc tggctggccg gtaacgtcgg gacgttcgcg cgttccgccg
     2461 cctcgcgggt cgcgatcttg tcgccgcaga gcacg
//
LOCUS       DASUGJ010000804         6332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1937123, whole genome shotgun sequence.
ACCESSION   DASUGJ010000804 DASUGJ010000000
VERSION     DASUGJ010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937123"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFV93_18140"
     CDS             complement(<1..906)
                     /locus_tag="VFV93_18140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX5167334.1"
                     /translation="MKRRTRRQNPWKKGYARPNRRERVAVYPLARHIETHPSRDSHHR
                     FDATTEFHVPAHNQPLAGSFRDQPRGVRFVAWMILIAVPLVTLAWVGSVVIPIAWEAQ
                     QAAGKVFVTPVARVSYVTPVATQPAAAGETPTSAAAVDPTQAPAMTATAGRQATTEPT
                     VVPTALPEWSGRDPINILLLGVDTRIGDTGPPRSDTIIIVHVDPVAKRVDMLSIPRDL
                     LVEIPGYYATKINAAYPFGELDASIPGGGPTLVAQTIELNFQIPIHYFAEVDIAGMEK
                     VVDTIGGVMIDVPGIVKDDQDPTENS"
     gene            complement(1028..1633)
                     /locus_tag="VFV93_18145"
     CDS             complement(1028..1633)
                     /locus_tag="VFV93_18145"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5167335.1"
                     /translation="MARSAADYWDEAMDAGAYIQQMTQNRELFERRIAATTIGASERA
                     AFSGRPLRVLALTEDFCGDSAQFIPPVIRLAQELDNVEIRLLLRDRYRELASGYVRKD
                     GYQAIPVLILLAEDGEELGYLVERPARANDALASETRRFARENPQLEGVQRTYANMPE
                     ETKAAVRANSNRFRDGQQAEWTGWLFEDLAAIVAQPAGVAD"
     gene            1724..2068
                     /locus_tag="VFV93_18150"
     CDS             1724..2068
                     /locus_tag="VFV93_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167336.1"
                     /translation="MSAPIAVSVLTRDVIAQVREIAQALPYRSSVGVDPERVVWLDVA
                     GEERIGVAYTPDDPDGPHWMIAFMRPDGRRVGKSAIGRVVDLIAGNGSGWEKAPPLDS
                     APHLTLVRVRAR"
     gene            2247..2924
                     /locus_tag="VFV93_18155"
     CDS             2247..2924
                     /locus_tag="VFV93_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167337.1"
                     /translation="MTTRPDAQILADCDDTDTLVAALAPLTGAIWLATDLTTLVHWTF
                     MAGSVDVVPEPAVGRICRAFGLVTVCAPSGEPVFLINQGDALEWIEPGQVLAVREMDD
                     PALEIRIERLDDLDARLISAHHIGSDCDTTTFRTISAGSGQYVQIYDEQLPTGDAGTD
                     AFLLMIESTIDARRRLIGPTAGTLEVMHQLACDLEQTLRMHPIRSYIWAAVLREQIAS
                     MRQLLAA"
     gene            3049..4962
                     /locus_tag="VFV93_18160"
     CDS             3049..4962
                     /locus_tag="VFV93_18160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5167338.1"
                     /translation="MTHRRRLTAEILADLTMPSEPQVSPDWSWLTASVTTTAKPEQDR
                     NAAIWLADTSGQAAARQITTGLTSDTHPRWSPDSRQLAFLSDRRKRGKSQLQLLSLDG
                     GEASELTDVRGGVAGFAWLPGDERRIAYLALDARDEEEEKRRDERDDANVYGAFWPLA
                     RIVVRDLDSGDEWRIDTGNFHVREIEPSPDGNLLGATISDTPEIDSGLATGRLVTIDL
                     RNGHVREIVPAGIGPSSVVWSLDGRRLFFIGAVGPTWVSSHQLFVVDADGGGEPHCVT
                     LDAPYCFSSLTRAEDGDDILALVATGVESEVYRIDPESFERTLVTHVAGDVSGLTASH
                     DGSVLGMIASTPDRPDDLYAMHEGGAPQRVSDLHAVLDDIAFGPQEVLTWERSGVGLD
                     GILIWPPDTSRADGPLPCVVSIHGGPYGRWPNEFITRFGRWIAQHGYLVFLPNPRGGA
                     GHGVEFAGSVRDTVGNDDYLDIMSGVDLLIEQGLADPVRIGCGGWSQGGFMTAWIVGH
                     TDRFKAGVMGAGVSDWGMMIATSDIPAYEQHMGGGNPYEGVGPHSFDAQSPASFVSKA
                     TTPVLIVHGENDERVPVTQGIHFFRGLRRYGVPTELVTYPREPHGIQERNHQIDLNNR
                     IIDWFQRWIPITA"
     gene            5124..>6332
                     /locus_tag="VFV93_18165"
     CDS             5124..>6332
                     /locus_tag="VFV93_18165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5167339.1"
                     /translation="MADVSIRDNGLSAPPTPLTRRERVRAMADTYPRAVWMMALCNLI
                     LWTGRGIVIPFTVIYFSQIVGLSASVVGAGIAAAGLGGIAFVVVVAGQIDRRGGHPVL
                     LACMSVLALATIGYPWATNVGLFLVVTIVLTFASQLYWPSSDATIASITDPARVAEAM
                     SILRVANAVGIGAGGFIGGVLAAGGGMSEYRVVFLTSAAFIITAALLVWRTVPAVILP
                     SKSNDGEHGTWADILPDRTFLYALLILFTLVFGFTQITMSVPAFLRAEAGIGEGTIGL
                     LFTMNTIVVVLTQIPITARLSKRHISKPLAFGAILWAHSFFWLLLTPTIGVPAAIVAF
                     LSFTAGELLFMPITALIPVRLAPVHLRGRYFSLSSVVWGGSYALASLVGGVALDMSNP
                     ALLWPVMIGLM"
BASE COUNT         1008 a         2141 c         2043 g         1140 t
ORIGIN      
        1 ggagttctcg gtcggatcct ggtcgtcctt gacgatgccc ggcacgtcga tcatgacgcc
       61 gccgatcgtg tcaacgacct tctccatccc ggcgatgtcc acctcggcga agtagtggat
      121 cgggatctgg aagttcagct cgatcgtctg ggcaaccagc gtcggcccgc cgccgggaat
      181 cgaggcgtcc aactcgccga acggataggc ggcgttgatc ttggtcgcgt agtagccggg
      241 aatctcaacc agcaggtcgc gcgggatcga gagcatatcg acccgcttcg cgaccggatc
      301 aacgtgaacg atgatgatcg tgtcgctgcg cggcggccct gtgtcgccga tccgcgtatc
      361 gacgccgagc agcaggatgt tgatcggatc gcggccggac cactccggca gcgccgttgg
      421 cacgaccgtc ggttccgtcg tcgcctgcct gcctgccgtc gccgtcatcg caggcgcctg
      481 ggtcgggtcg accgcggctg cgcttgtcgg cgtctcgcct gccgctgcgg gttgggtcgc
      541 taccggcgtc acgtacgaga cgcgcgcgac cggcgtgacg aagaccttgc cggccgcctg
      601 ttgcgcttcc cacgcgatcg ggatcacgac cgagccgacc caggccagcg tcacgagcgg
      661 gacggcgatc aggatcatcc aggcgacgaa acgcacaccg cgcggctgat cccggaatga
      721 gcctgccagc ggctgattgt gagccgggac gtggaactcg gttgtcgcat cgaagcggtg
      781 gtggctgtcc ctggatgggt gtgtctcgat atgtcgcgca agcggataga ccgccacgcg
      841 ctcacgccgg tttggccgcg cgtagccctt tttccatgga ttctggcgtc tggttcgtcg
      901 cttcatcagc tccggccgcg tacgaacggt tcaacatgct attgtaaacc atgttcgtac
      961 agatccgtca cgatctggcc acgacgaagc agtgtcccgt cacgcaataa aggtgttacg
     1021 cgtcctgtca gtcagcgacg ccggccggtt gggcgacgat cgcggccaga tcctcgaaga
     1081 gccagccggt ccactccgcc tgctggccgt cacggaatcg gttcgaattg gcgcgcacgg
     1141 cagccttcgt ctcctccggc atattggcat acgtgcgctg gacgccttcg agctgcgggt
     1201 tctctctggc gaatcggcgt gtctcggagg ccagtgcgtc gttggcccgc gccggccgct
     1261 cgacgaggta gccaagttcc tcgccatcct cggcgagcag gatcaggacc ggaatcgcct
     1321 ggtagccatc cttgcgcacg tagccggaag ccagctcccg gtaacggtcg cgcaacagca
     1381 gccgaatctc gacgttgtcc agctcctgcg cgagccggat caccggcggg atgaactggg
     1441 ctgagtcacc gcagaagtct tccgtcagcg ccagaacccg tagcggccta ccggagaacg
     1501 ccgcccgctc gctggcgccg atcgttgtgg ccgcgatccg gcgctcgaag agttcacggt
     1561 tctgcgtcat ctgctggatg tacgcgccgg cgtccatcgc ctcatcccag tagtcagcgg
     1621 ccgatctcgc catgttccct cctccatgtg gcatcgttcc tgttgtctgt aacctctggt
     1681 ccgggctgct attccggatc gatggctccg gaaggaacgc cagatgtccg cgccgatcgc
     1741 cgtctcagtt ctgacccgcg acgtcatcgc gcaggttcgc gagatcgcac aggcgctgcc
     1801 gtaccggtcg agcgtcggtg tcgatccgga gcgcgtcgtc tggctggacg tcgccggcga
     1861 ggaacggatc ggcgtcgcct acacgcccga cgaccccgac ggcccgcact ggatgatcgc
     1921 cttcatgcgg ccggacggtc gccgcgtcgg caagagcgcg atcggccggg tcgtcgatct
     1981 aatcgccggc aacggcagcg gctgggagaa ggcgccaccg ctcgacagcg cgccgcatct
     2041 caccctcgtt cgggtacgcg cacgctgatc ccaccttgcc ttctgatgtc tctgtgacac
     2101 tcttgtgacg ctgaaagccc gaatgttgct tccatgcccc ggctcgcttc ctcacaatta
     2161 ggaagccagg cggcaggacc ggctggggcg ctggaccgaa gcggatcgat ccagcactgg
     2221 acgcagccgt cggagaccag agccagatga cgacccggcc tgacgcacag atcctcgcag
     2281 actgcgacga caccgacaca ttggtcgctg ccctcgcgcc gttgacaggg gcgatctggc
     2341 tggcaacgga cctgacgacc ctcgtccact ggaccttcat ggccggcagc gtcgacgtcg
     2401 tgccggaacc ggcagtcggc cgtatctgcc gcgcgttcgg cctcgtcacc gtctgcgcac
     2461 ccagtggtga accggtcttc ctcatcaatc agggtgatgc attggagtgg atcgagccgg
     2521 ggcaggtgct ggctgtccgc gagatggacg atcctgcgct ggaaatccgc atcgagcggc
     2581 tggacgacct cgatgcgcgg ctgatctccg ctcaccatat cggcagcgat tgcgacacaa
     2641 caaccttcag aaccatctcc gccggctctg gccagtacgt acagatctat gacgagcagc
     2701 tacccaccgg cgacgccggc accgacgcct tcctgctcat gatcgagtcg acgatcgacg
     2761 ctcgccggcg gctgatcggc ccaactgccg gaacgctgga ggtcatgcac cagctcgcct
     2821 gtgatctgga acagacgctg cggatgcacc ccatccgctc ctacatctgg gcagccgtcc
     2881 ttcgcgagca gatcgccagc atgcgccaac tcctcgccgc ctgaaaccgg ctgcgtctta
     2941 tctggcacgg aaccccttcg gggccgtgcc tcggtgccgg ccgcgaaggg tacatggtgg
     3001 gcatcctgct acactcagcc ggctggaccg atgacgagag gaaaagagat gacgcatcgc
     3061 aggcgattga ccgccgaaat ccttgctgat ctaacgatgc cgtctgaacc gcaggtcagc
     3121 cccgattgga gctggctgac ggcgtcggtg acgacgactg ccaagccgga gcaggaccgg
     3181 aacgctgcga tctggctggc tgacacgagc ggccaggcag cagcacgcca gatcacgacc
     3241 ggattgacga gtgacaccca tccccgctgg tcgcctgata gccgccagct cgcgttcctc
     3301 tccgaccggc gcaagcgtgg caagagccag ctacagctgc tgtcactgga tggcggcgag
     3361 gcatccgaac tgaccgacgt gcgcgggggc gtagccggtt tcgcctggct gccgggcgac
     3421 gagaggcgca tcgcctactt ggcactagac gcccgcgatg aggaagagga gaagcgccgc
     3481 gacgaacggg acgacgcgaa tgtctacggc gcgttctggc cgctggcccg gatcgtcgtc
     3541 cgcgacctcg attccggcga cgagtggcgg atcgacacgg gcaacttcca cgtccgggag
     3601 atcgagccat cgccggacgg caatctgctg ggtgcgacca tctccgacac gccggagatc
     3661 gacagcgggc tggcgaccgg ccggctggtc acaatcgatc tgcgtaacgg gcacgttcgc
     3721 gagatcgtcc cggccggcat cggaccgtca tccgtcgtct ggagcctcga cggccggcga
     3781 ctcttcttca tcggcgcggt cggcccaacc tgggtcagct cccatcaact ctttgttgta
     3841 gacgcggatg gcggcggaga gccgcattgc gtcaccctcg acgcgccgta ctgcttctcg
     3901 tcgctgacca gggcggagga cggcgacgac attctggcgc tggtcgcaac cggcgtcgaa
     3961 tctgaggtct accgcatcga ccctgaatcc ttcgagcgaa cgctcgtcac tcatgtcgcc
     4021 ggcgacgtca gcggcctgac ggccagccac gacgggtcgg tcctcggcat gatcgccagc
     4081 acgcctgacc gacccgacga tctctacgca atgcacgagg gcggggcacc gcagcgcgtc
     4141 tcggacctgc acgccgtcct ggatgacatc gcgttcggtc cacaggaggt actgacctgg
     4201 gaacgctccg gcgtcggtct ggacggcatc ctgatctggc cgcccgacac gagccgcgcc
     4261 gacgggccgc tgccgtgcgt cgtctcgatc cacggcgggc cgtatggccg ctggccgaac
     4321 gagttcatca cccgcttcgg ccgctggatc gcccagcacg gctatctggt cttcctgccc
     4381 aatccccgcg gcggggccgg tcacggcgtc gagttcgccg gttcggtccg cgacacggtc
     4441 ggcaacgacg actacctcga catcatgtcg ggtgtcgatc tgctgatcga gcaggggttg
     4501 gccgatccgg tccgcatcgg ctgtggcggc tggagccagg gcggcttcat gacggcctgg
     4561 atcgtcggcc acaccgaccg cttcaaggcc ggcgtgatgg gcgccggtgt ctccgactgg
     4621 ggcatgatga tcgccaccag cgatatcccc gcctacgagc agcacatggg cggcggcaat
     4681 ccctacgagg gcgtcggccc gcactcgttc gacgcacaga gcccggcgtc attcgtctcg
     4741 aaggccacga cgccggtcct gatcgtccac ggcgagaacg atgagcgcgt cccggtgacg
     4801 caaggcatcc acttcttccg cggcctgcgc cgctacggcg tcccaaccga gttggtcacc
     4861 tatccccgcg agccacacgg catccaggag cgcaaccacc agatcgacct caacaaccgg
     4921 atcatcgatt ggttccagcg gtggattccg ataaccgcgt gacgcgggac gcggggcgcg
     4981 acggcgatcg tcccgcgtcc cgtcgctacg acattcttcc gatgcgccac tgaccccggt
     5041 ctgcaatgct taggcgatgc cggtgcgatg tcgcgccggc atcgcgggca catggtcggt
     5101 gcattgagaa gcagaggatt cggatggcag acgtttcgat ccgcgacaat gggttgagcg
     5161 ccccgccaac gccgcttacc cggcgcgagc gggtgcgggc gatggcggat acctacccgc
     5221 gcgcggtctg gatgatggcg ctctgcaacc tcatcctctg gaccggacgt gggatcgtca
     5281 tcccgttcac cgtcatctac ttcagccaga tcgtcgggct ctcggccagt gttgtcgggg
     5341 ccggtatcgc ggcggccggg ctgggcggga tcgcgtttgt cgtcgtcgtg gccggccaga
     5401 tcgaccggcg tggcggccat ccggttctgc tggcctgtat gtcggtcctc gcgctggcga
     5461 cgatcggcta tccgtgggcg acgaacgtcg ggctgttcct ggtcgtgacg atcgtcctga
     5521 ccttcgccag ccagctctac tggccgtcgt ccgacgcgac gatcgcgtca atcaccgacc
     5581 cggcccgtgt cgccgaggcc atgtcgattc tgcgggttgc caatgccgtc gggatcggcg
     5641 ccggcggctt catcggcggt gtgctggcag ccggcggggg gatgtccgaa taccgcgtcg
     5701 tctttctgac cagcgccgcc ttcatcatca cggccgcgct gttggtctgg cggaccgtcc
     5761 cggccgtgat cctgccgtcg aagtcgaacg acggagagca cggcacctgg gcggacattc
     5821 tgccggatcg aacgttcctc tacgcgctgc tgatcctctt cacgctcgtc ttcggtttca
     5881 cccagatcac gatgagcgtg ccggccttcc tgcgcgccga ggcggggatt ggcgagggca
     5941 cgatcggctt gctgttcacg atgaacacga tcgtggttgt cctgacccag atcccgatca
     6001 cagctcgcct cagcaagcgc cacatcagca agccactggc gttcggtgcc attctctggg
     6061 cacattcatt cttctggctg ttactgacgc cgacgatcgg ggtccccgcc gccatcgtcg
     6121 cgttcctctc gttcacggcc ggtgagctgc tgttcatgcc gatcacggcg ctgatccctg
     6181 tccggctggc gccggtccac ctgcgcgggc gctatttctc gctgtccagc gtcgtctggg
     6241 gcggctcgta cgcgctggcc tcgctggttg gcggcgtcgc gctcgacatg tccaacccgg
     6301 ccctgctctg gccggtcatg atcggactga tg
//
LOCUS       DASUGJ010000805         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_409553, whole genome shotgun sequence.
ACCESSION   DASUGJ010000805 DASUGJ010000000
VERSION     DASUGJ010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409553"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFV93_18170"
     CDS             complement(<1..358)
                     /locus_tag="VFV93_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167340.1"
                     /translation="MKSANEFDARRRPTPLFETEVAPVSVQIQLLGGFRVVVDGRVVP
                     ETAWRLGNARRLIQVLALAPNHGLHREQIMDLFWPDLTPSAAASNLYQVVHAARRALN
                     ACYAPRGKPAAIIHLRQ"
     gene            667..1293
                     /locus_tag="VFV93_18175"
     CDS             667..1293
                     /locus_tag="VFV93_18175"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5167341.1"
                     /translation="MESSRFIEPERAALVLIDVQPLFLDLMAGEQEPLLARLEYLLLL
                     AQTFAMPVIATFEQPVERNGWLPERLERIFPHDGRRYIKQTFNLCSEPEIRAAVEATG
                     REQMIVAGAETDVCVLQSVLGLLDLGQQVFMVEDGLFTAEPNDAPALRRMEQAGAIPL
                     TCKTLYYELKRSVAAPAFHLEWNRHYGDGDSAYVDPYDLPPSRRLRSD"
     gene            complement(1478..2407)
                     /locus_tag="VFV93_18180"
     CDS             complement(1478..2407)
                     /locus_tag="VFV93_18180"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5167342.1"
                     /translation="MSWLARKETPLTDGAVWPERLREAIERHTGGRFGVERLSGMSFS
                     GVWRVSGAAGSTIVKASQSGYEASFYEGVASSLRRAGVPIPDLLLALDDGTRHWLLLE
                     DIPEPLPVASGPDWQPDGQIVEILARLHATTRARPPRLPNPPARNWTDAMTQSALGCL
                     TDQGTDLEPLLATMQHEAQELSQPWCWISGDPNPRNWGLRGGRQPVLFDWELFGPGLP
                     AIDLAIIVPGLGDAAAYRRVADAYDAVWPGPDPLPWDTDQLGHQIAVAKVATVVQLLD
                     SHATGRAEVGDELLDWLADSVPTWVGQIARSNH"
     gene            complement(2404..>2817)
                     /locus_tag="VFV93_18185"
     CDS             complement(2404..>2817)
                     /locus_tag="VFV93_18185"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5167343.1"
                     /translation="EGFTRYAGYPDFRGFLACVDGEPVGVGYGARSFPGVPWHDLVAP
                     RLGAEHPALQDAFRIVELAVIDSRRGQGLGGLLHDRLLKSQLCPRALISTNVTNQLAR
                     GLYERRGWRYLHTAFDVPDDPHRYVIMARELRAGA"
BASE COUNT          467 a          915 c          967 g          468 t
ORIGIN      
        1 gctggcgcag gtggatgatc gcggccggct tgccgcgcgg cgcgtaacag gcgttgagtg
       61 cacgccgggc ggcatgcacg acctggtaga gattactggc cgctgcgctc ggcgtgaggt
      121 cgggccagaa caggtccatg atctgctcgc gatgcaggcc gtggttcggc gccagcgcca
      181 ggacctggat caggcgacgt gcgttgccga gccgccaggc ggtctccggc accacccggc
      241 catcgacgac gacacgaaag ccgccgagca actgaatctg gaccgagacc ggagcaacct
      301 cagtctcgaa cagtggtgtc ggcctgcgac gcgcgtcgaa ctcgttcgcg gacttcatgc
      361 ctggtctgtc accactcgtc atgcgctggc gaccgaaatc gccctgggaa tgccagcgga
      421 ttcgtgtagt tggcgtcccg catattgtac gtgccaggcg tgctgatggt gcgctctgcg
      481 gaggtactct ggtgggaaaa tttcctgccg cgtagccggc tccgccgtcg gaaacagggt
      541 cgcctgatac agccaggctg catcgagatg gcgcaacatg ttgggatgta ccctggtggt
      601 gctgcctcgt tgcctcttgc acaccctgca cggtacagtg cgcttgatgt caaggggagt
      661 gtgaaagtgg aatccagtcg attcatagag ccggagcggg cagcgttggt gctgatcgat
      721 gttcagccgc tgttcctcga cctgatggcc ggtgaacagg agccgctact ggcgcggctg
      781 gagtacctgc tattgctggc gcagacgttc gcgatgccgg tgatcgcgac tttcgagcaa
      841 cccgtcgagc ggaacggctg gctgccggag cggctcgaac gaatcttccc gcacgacggc
      901 agacgctaca tcaagcagac cttcaacctc tgctcggagc cggagatccg ggcggccgtc
      961 gaggccaccg gccgcgagca gatgatcgtc gccggcgcgg agacggatgt ctgtgtcctg
     1021 caatcggtgc tcgggctgct ggatctgggc cagcaggtgt tcatggtcga agatggcctc
     1081 ttcacggccg agccgaacga cgcgccggcc ctgcggcgga tggagcaggc tggggcgatt
     1141 ccgctgacct gcaaaacgct ctactacgag ctgaagcggt cggtcgccgc cccggcgttt
     1201 catctggagt ggaaccgcca ctacggcgac ggcgacagcg cgtatgtcga tccctacgat
     1261 ctgccaccgt cgcgtcgatt gcgctcggat tgattggcga gcgtggcgtc tagccgggcg
     1321 tgaagatcgc gtgggcggga tagatctcga cctccggctg gcccggcaga ccagcctgtg
     1381 tgacaccggg catcagccgc tggtcgacga acgcctggaa ctgctcgccc gattcccaga
     1441 tatccgtgac gcgcagtgcc ttaccatgcg cgttggttca gtgattcgag cgagcaatct
     1501 gcccgaccca ggtcgggacg gaatcggcca gccagtccag cagttcatcg ccaacctcgg
     1561 cgcgtccggt ggcgtggctg tcgagcagtt ggacgacggt ggcgaccttc gcgacggcga
     1621 tctggtggcc gagctgatcg gtgtcccacg ggagtgggtc ggggccgggc cagacggcgt
     1681 cgtaggcgtc agcgacgcga cggtaagcgg cggcatcacc gaggccgggg acgatgatcg
     1741 ccaggtcgat ggccggaagg ccagggccga acagctccca gtcgaacagg accggctgcc
     1801 tgcctccccg cagcccccag ttgcgcgggt tgggatcgcc ggagatccag caccatggtt
     1861 gagacagctc ctgcgcctca tgttgcatcg tcgccagcag cggttccagg tccgtcccct
     1921 gatcggtgag acaaccgagc gcggattgag tcatcgcgtc ggtccagttg cgcgccggcg
     1981 gattgggcag ccggggcggc cgggcgcggg ttgtggcgtg gagccgggcg aggatctcca
     2041 cgatctgccc gtcaggctgc cagtccggcc cactcgcgac cggcagcggc tcggggatgt
     2101 cttcgagcag cagccagtgg cgcgttccgt cgtccagcgc cagcagcaga tcggggatcg
     2161 gaacccccgc ccgccgcaac gacgatgcga caccttcgta gaagctcgcc tcatagccgg
     2221 attggctcgc cttgacaatc gtggagccgg ccgcaccgct cacgcgccag acgccgctga
     2281 aactcatccc gctcagccgc tcgacaccga agcggccacc cgtgtggcgc tcgattgctt
     2341 cgcgaagcct ctcgggccag actgctccgt ccgttagcgg tgtctccttc cgggccaacc
     2401 agctcaagcg ccggcccgca gctccctggc catgatgacg tagcggtgcg ggtcgtcggg
     2461 tacatcgaac gcggtgtgca gatagcgcca gccgcgccgc tcgtagagcc cgcgcgccag
     2521 ctggttggtg acgttagtcg aaatcagtgc cctcgggcat agctggctct tcagcagccg
     2581 atcgtgcagc aacccgccga gcccctgacc ccgccggcta tcgatgaccg ccagctcgac
     2641 gatgcggaac gcgtcctgca aggccggatg ctccgccccc agccggggcg cgacgagatc
     2701 gtgccagggc acgccgggaa acgagcgtgc cccgtagccg acgccgaccg gctcgccatc
     2761 gacgcaggcg aggaagcccc ggaagtccgg atagccggcg tagcgggtga acccctc
//
LOCUS       DASUGJ010000806         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1243192, whole genome shotgun sequence.
ACCESSION   DASUGJ010000806 DASUGJ010000000
VERSION     DASUGJ010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243192"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..580
                     /locus_tag="VFV93_18190"
     CDS             233..580
                     /locus_tag="VFV93_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167344.1"
                     /translation="MAELLRCVDRWGREVVLYEATWYDKIVVDHIDMVDRFDAIIAVL
                     AHPERVHFDATYANGENFYGRDYPDHPFSPDYLKVVVRYELIGEVEVGTIVSAYPAPH
                     YRKHGERFKWQRR"
     gene            565..966
                     /locus_tag="VFV93_18195"
     CDS             565..966
                     /locus_tag="VFV93_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEX5167345.1"
                     /translation="MATTVASIADILGFEPIFPRPEEIDSARLVISYNDAADTLYVHF
                     FERGEPATSIDVDECLYLRVNRMSRRVVGLQIEGFLIRAVHEHPDWLSLAELAGIPAR
                     MIDEARASIDAGERRRAVVLPILRELNLLAT"
     gene            complement(987..2189)
                     /locus_tag="VFV93_18200"
     CDS             complement(987..2189)
                     /locus_tag="VFV93_18200"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5167346.1"
                     /translation="MTVAESAPATTASVIRNRPLITLALGHFTIDLYAGLLPVLFPLL
                     EREYDLDLATVGLIALAYSGMGSISQPLFGWLADRHGTRLIGAALVWTAVTFSGIAFA
                     PSFPVILVLAGLAGLGSGAFHPFGALNANAVISPAKRNAAMSVYASGGTLGFALGPLI
                     GVALLAILGQHGIVLMLIPGTAIAAWLLYEMRTIAVHGRGRGKTIDATLPPIPKLAMA
                     AVIIVMMSRSWMMSSIQAFVPSWYDDLGYSTAFYGALATTITLSSAAGTIGSGSLADR
                     YGRRALIIGSLVLTMPTVLLFAQFTGPIAFVSGALVGLLAASTAPLLLVMAQQLMRGR
                     AGVASGLILGLGFVTGALGVPITGAVADAIGIQDAMRLQTIVVLIAIPVALWLPTEAK
                     IEAMHARQ"
BASE COUNT          425 a          696 c          737 g          389 t
ORIGIN      
        1 ctcggtcatc agcaaccgcc ccaattcgta gtctcttccg ccgtcgacca tcccctgact
       61 ccgatcgaca acgccggcaa ccccctcaat atcgaccgag atgtagacct tcatgatcgt
      121 gcctccctct tgcgtgagcc atcgtggcag gcgcagacga tagcacggtt cccacttcgc
      181 tatactgcga tatcgagcga ggatcgaagc gtacaatgga ggccaactcg gcgtggctga
      241 gcttctgcgg tgtgtggacc gatggggccg tgaggtcgtt ctgtatgaag ccacctggta
      301 tgacaagatc gtcgttgatc atatcgacat ggtggaccgg tttgacgcca tcatcgctgt
      361 tctcgctcat ccggaacgag tgcatttcga cgcgacgtat gcaaacggcg agaacttcta
      421 cggacgcgac tatccagatc atccgttcag tccggactac ctgaaggtcg ttgtgcggta
      481 cgaacttatt ggcgaagtcg aggtcgggac gatcgtttcc gcctatcctg ctccgcatta
      541 ccgaaagcat ggtgaacgat tcaaatggca acgacggtag cgtccatcgc tgacatcctg
      601 gggtttgagc cgatcttccc gcgaccggaa gagatcgact ctgcgcgcct ggttatctca
      661 tacaacgatg cggccgacac gctctacgtt cacttctttg agagaggtga gccagctacc
      721 agcatcgacg ttgatgagtg tctctatctg cgggttaacc gaatgagtcg gcgcgttgtc
      781 ggtttgcaga tcgaaggctt cctgattcga gctgtccatg agcatcccga ctggctctcg
      841 cttgcagagt tggcgggcat tccggcgcgc atgatcgacg aggctcgtgc gtcgatcgat
      901 gccggtgagc gtcgtcgggc cgtcgtcctc cctattctcc gggaactcaa tctgctcgcg
      961 acctgatccg cggcgccgct gcatcactac tgccgagcgt gcatcgcctc gatcttggcc
     1021 tcggtcggga gccagagggc aaccgggatg gcgatcagga cgacgatggt ctggaggcgc
     1081 atggcgtcct ggatgccgat tgcgtcggcg acggcgccgg taatcgggac gcccagcgcc
     1141 ccggtgacga agccgaggcc gaggatcagg ccgctggcga cgccggcccg gccgcgcatc
     1201 agttgctggg ccatcacgag cagcagcggc gccgtcgacg cggccagcag gccgaccagc
     1261 gcgccgctga cgaaggcgat cggcccggtg aactgggcga agagcaggac agtcggcatc
     1321 gtcaggacga gtgagccgat gatcagtgcc cgacggccat agcggtcggc cagtgagccg
     1381 gagccgatcg tgccggccgc gctggagagg gtgatcgtcg tcgccagtgc gccgtagaag
     1441 gcggtcgagt agccgaggtc gtcgtaccag gacggaacga acgcctggat gctcgacatc
     1501 atccacgagc gcgacatcat gacgatgatg acggccgcca tcgccagctt cgggatcggc
     1561 ggcagcgtcg cgtcgatcgt cttgccgcgc ccgcgtccgt ggacggcgat cgtccgcatc
     1621 tcgtacagca gccaggcggc gattgccgtg ccgggaatca gcatcaggac gatgccgtgc
     1681 tggcccagga tcgccagcag cgcaacgccg atcagcggcc cgagcgcgaa accgagcgtc
     1741 ccgcccgagg cgtagaccga catcgccgcg ttgcgcttgg ccggcgagat gacggcgttg
     1801 gcattcaagg cgccgaaggg gtggaaggcg ccggagccga gccctgccag cccggccagg
     1861 accaggatca ctggaaacga cggcgcgaag gcgatgcccg agaacgtcac cgccgtccag
     1921 accagggcgg cgccaatcag tcgcgtcccg tgccggtcgg ccagccagcc gaacagcggc
     1981 tgcgagatcg agcccatccc gctataggcc agcgcgatca gcccgaccgt cgccagatcg
     2041 aggtcgtact cgcgctccag caacggaaag agcaccggca gcaagccagc atacaggtcg
     2101 attgtgaagt gtcccagcgc caacgtaatc agcgggcggt tgcggatgac gctcgccgtt
     2161 gtggccggtg cggactcagc gacggtcacg ggcgcgcgtt cctgggcatg gggaatgggg
     2221 gatgaggcat gaggagcaat cttgact
//
LOCUS       DASUGJ010000807         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1243197, whole genome shotgun sequence.
ACCESSION   DASUGJ010000807 DASUGJ010000000
VERSION     DASUGJ010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243197"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1230
                     /locus_tag="VFV93_18205"
     CDS             232..1230
                     /locus_tag="VFV93_18205"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX5167347.1"
                     /translation="MTGIAIHGFGRIGRSTLKAALRDERFVPVSVSDIRDTPTFAALF
                     KADTNYGLWPEEVKARDNGFVIGGREIRYVNSMDQLPDWSALGVGLVIDCTGRATTRE
                     GAQAHLDRGAKRVLVSAPSKSLEDCDAVLLPGVNLDQFDPDAHRIISMASCTTNSLAT
                     VIKVLQEQFALERGFFTTVHAYTNSQALTDQPMKDRRDSWAAAENIIPSSSGAAKALQ
                     FIWPNLNVTGKAYRVPARTGSIAELNAVLGRATTADALRDAFRKAAAGPMQGVMDVLE
                     EEWTSARIIGDPHAALVDLPLIQVLDGTLITVASWYDNEMGYSTQLAATAANLAGR"
     gene            1453..1821
                     /locus_tag="VFV93_18210"
     CDS             1453..1821
                     /locus_tag="VFV93_18210"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5167348.1"
                     /translation="MFDERIPIYRQIEDQIKADILSGALAEDQQVMSTNQYAAFYRIN
                     PATAAKAFQHLEAEGVLYKRRGIGMFVSPNARERLLAQHRASFFSDVVDPMLATARTI
                     EIPVDDIIDHIRQQAGKDAR"
     gene            1818..>2402
                     /locus_tag="VFV93_18215"
     CDS             1818..>2402
                     /locus_tag="VFV93_18215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5167349.1"
                     /translation="MTMAVETRGLTLRYGEKTALDNLSFALDGGKIYGLLGRNGSGKS
                     SLLSVLAAFRKADAGEVLVGGEPVFENPAITEQICLIRESVDVFERSATIESVLGFTS
                     AIRPGWDAEYAGRLLDAFELDPRQKVKDLSRGKQSALGAILGLASQAPLTMFDETYLG
                     MDAPSRYCFYEELLADYIAHPRTMIVSTHLIEEVA"
BASE COUNT          453 a          772 c          772 g          405 t
ORIGIN      
        1 acctcgccga caaccgggtc cggagcgggg gtggtagctg cgggtgcgac agacatcgcg
       61 gcacactcct cggctaacaa ccagtcgaac cacagagcct accgcgattt agttggaacc
      121 ggtgtggccg cagcctcgcc cctacgcgag aacaggaacg gccaacgcgg cacacttcat
      181 gatgacgcat cgccaggatc gatcatcgca acactgaggg agggcgacat catgacagga
      241 atcgcgattc atggttttgg acgaatcggg cgctcgacgc tgaaggcggc cttgcgggat
      301 gagcgtttcg tgccggtctc cgtttcggac attcgcgaca cgccgacctt cgcggccctc
      361 ttcaaggccg atacgaacta cggcctctgg ccggaggagg tcaaggcacg cgacaacggc
      421 ttcgtcatcg gcgggcgcga gattcgctac gtgaactcga tggaccagtt gccagactgg
      481 tcggcgctgg gcgttggtct ggtcatcgac tgcaccggcc gggcgacgac ccgcgagggt
      541 gcccaggccc atctcgaccg gggcgcgaag cgggtgctgg tgtcggcgcc gagcaagtcg
      601 ctcgaagatt gcgatgcggt cctgttgccg ggtgtcaacc tcgaccagtt tgaccccgac
      661 gcgcatcgga tcattagcat ggccagctgc acgaccaact cgctggcgac cgtcatcaag
      721 gtcctgcagg agcagttcgc gctggagcgc ggcttcttca cgaccgtcca cgcctacacg
      781 aactcgcagg cgctgaccga ccagccgatg aaggaccggc gcgactcgtg ggcggctgcc
      841 gagaacatca tcccgtcctc ctcaggcgcg gccaaagccc tccagttcat ctggccgaac
      901 ctcaacgtca ccggcaaggc ctaccgtgta ccggcccgca ccggcagcat cgccgagctg
      961 aacgccgtcc tcggccgggc gacgaccgcc gacgcgttgc gcgacgcctt ccgcaaggcg
     1021 gccgccggcc cgatgcaggg cgtcatggac gtcctcgaag aggagtggac ctcggcccgc
     1081 attatcggcg acccacatgc ggcgctggtc gatctgccgc tgatccaggt cctcgacggc
     1141 acgctgatca ccgtcgcgag ctggtacgac aacgagatgg gctactcgac ccagctggcc
     1201 gcgacggcag caaatctggc gggccgctag gccgggaatc caaatccaat cgtcaatccc
     1261 aatccggtac gggcgatatt gcgatatcgc cctactcacc cgcgcgaacg tctcggtggg
     1321 gatgcagggc gatattgcga tatcgcccct accggattgc ccattggtca ttggtgctcg
     1381 ctttgacaac aggaacctcc ggtgctatgg ttcactggta atgtaatgaa ccgatgatgc
     1441 aacgaggttc atgtgtttga cgagcgaatc ccgatctatc gccagatcga agatcagatc
     1501 aaggcagaca tcctgagcgg agcgctcgcg gaggatcagc aggtgatgtc aaccaaccag
     1561 tacgcggcct tctatcgcat caacccggcg acggcggcca aggcctttca acatctggag
     1621 gccgagggag tgctctacaa gcggcgcggg atcggcatgt tcgtcagtcc gaacgcccgc
     1681 gagcggttgc tggcgcagca ccgcgccagc ttcttcagcg atgtcgtcga tccgatgctg
     1741 gcgacggcaa ggacgatcga gatcccggtt gacgacatca tcgaccatat ccggcaacag
     1801 gcgggcaagg acgcacgatg acgatggcag tggagacgcg cggcctgacg ttgcgctacg
     1861 gcgagaagac cgcgctggac aacctctcgt tcgcgctgga tggcggcaag atctacggtc
     1921 tgctgggccg caacggctcg ggcaagagca gcctgctgtc ggtcctggcc gccttccgca
     1981 aggcggacgc gggcgaggtg ctggtcggcg gcgagccggt cttcgagaat ccggccatca
     2041 ccgagcagat ctgcctcatc cgcgagagcg tcgatgtctt cgagcggtcg gcgacgatcg
     2101 aatccgtcct cggctttacc tcggcgatcc gccccggctg ggacgcggag tacgccggcc
     2161 gcctgctgga cgccttcgag ctcgatccgc gtcagaaggt caaggatctc tcacggggca
     2221 agcaatcggc gctgggggcg atcctcgggt tggcctcgca ggcgccgctg acgatgttcg
     2281 acgagaccta cctcgggatg gacgcgccct cgcgctactg cttctacgag gagctgctgg
     2341 ccgactacat tgcccatccg cgaacgatga tcgtctcaac tcatctgatc gaggaggtcg
     2401 cg
//
LOCUS       DASUGJ010000808         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1383395, whole genome shotgun sequence.
ACCESSION   DASUGJ010000808 DASUGJ010000000
VERSION     DASUGJ010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383395"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..446)
                     /locus_tag="VFV93_18220"
     CDS             complement(123..446)
                     /locus_tag="VFV93_18220"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5167350.1"
                     /translation="MGPRIRELRIERGMTLDELARAANISASHLSRLERGQTAPSFKV
                     AADIAAHIGVKPSELATIQREQSDVDQALIEALTARGLSDEIAQHICDKISTVARAAL
                     LEIVR"
     gene            868..1374
                     /gene="rpsP"
                     /locus_tag="VFV93_18225"
     CDS             868..1374
                     /gene="rpsP"
                     /locus_tag="VFV93_18225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX5167351.1"
                     /translation="MLKLRLRRMGAKRQPSYRIVVAESRSPRDGRFIETVGIYNPKTQ
                     PMTLRVDSERAKYWLERGAQPTDTVRSLLVRVGAVPGRISKEGLAEGYVTEVPSRGST
                     PEVMQAHGGTPEAAAATAAPAKKAKAATATAEPAAEVAAEAPAAVEEPAAEVAEETEE
                     TAEPETQA"
     gene            1546..1965
                     /locus_tag="VFV93_18230"
     CDS             1546..1965
                     /locus_tag="VFV93_18230"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX5167352.1"
                     /translation="MSSEDADNLTDERDDLDDVDDVDEYDDDYDEYDDDEDVDDEDDE
                     DGQPAGTGVALTGDAPIDELATFVEFMVSNLVEEPDDFRVVPERLGTNVHIKVIVPEE
                     EMGRVIGRQGRIARSMRTLLTIAASRKGLRASLDIDS"
     gene            2004..2690
                     /gene="rimM"
                     /locus_tag="VFV93_18235"
     CDS             2004..2690
                     /gene="rimM"
                     /locus_tag="VFV93_18235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX5167353.1"
                     /translation="MTDTPDAGTPTRPRTPWREARPPGPTNPDPNTPPAEVFFAVGRI
                     VGAHGIRGEAKMEIFTDDPDHLLDLRRVYFDDDPTPRRLSGVRFHARQALLTFPDITD
                     RDAADALRGTIVRISGSQAKPPNEGEFYHYQLIGLAVQDEAGNPLGTLTEILEAGEVD
                     TYVVRNEDGREQLFPALKDVVLDINPAENRVVVRPLIWEADTPRKPHNPDRPPRPRKL
                     RRPKAGPSTT"
BASE COUNT          564 a          948 c          873 g          442 t
ORIGIN      
        1 aaagtgctga atgcgtgacg cgtgacgcgt gatgcgtgac gcgtgtaatg gcgcaggtcg
       61 cgttgcaggt tccctctcaa cgcatcacgc gtcacgcgtc acgcatcacg cgtcacgaac
      121 acctaccgca caatctccag caacgccgcg cgggcgacgg ttgagatctt gtcgcagatg
      181 tgctgggcga tctcatcgct caggccgcgg gccgtcagcg cctcgatcag cgcctgatcg
      241 acatcggatt gctcgcgctg gatcgtggca agctcgctgg gcttgacgcc gatgtgggcg
      301 gcgatgtcgg ccgcaacctt gaacgagggg gctgtctgcc cccgttcgag ccgcgagaga
      361 tggctggcgg agatgttggc agcgcgagca agctcgtcga gcgtcattcc ccgctcgatc
      421 ctcagctcgc ggatccgtgg gcccattgaa ttccgcgttt tggcgggcac tcggtttctc
      481 cttactggcc cggccccgca ccctcgttgc ggatttgacc ggtttgcgta tcacagacac
      541 atgcacgttg aggcaaacgt caacgcaccg gaatcaccga ccctctggaa ccgtgctcag
      601 atgcgagaag tataaggcat gaaaggttga ccgcatagta gtaggaacat gcacggatgc
      661 ctttcatgtt tgtaagacag acgataccgc actctggcac atgcgagata tggacacggt
      721 agagaatgga tggcaagatc gttgacgcat tggcaccaac tggggtagca ttgagtgttc
      781 cggtcgcgaa ccggtcgtat tactgtgtgc tgtaaactgc gatcatgata ccgtgagaca
      841 gacgggtaca gaaggggaac tgccagcgtg ctgaaactgc gactgcggcg aatgggagcc
      901 aagcgccaac caagctatcg aatcgtcgtc gccgagtccc ggtcaccgcg cgatggccgc
      961 ttcatcgaga cggtcggcat ctacaacccc aagacccagc cgatgacgtt gcgcgtcgat
     1021 tccgagcgcg ccaagtactg gctcgagcgt ggggcccagc caaccgacac ggttcgctcg
     1081 ctgctcgtcc gcgtcggggc cgtccccggc cggatcagca aggaagggct tgccgagggc
     1141 tatgtgaccg aagtgccgag ccgcggctcg acaccggagg tgatgcaggc gcacggcgga
     1201 acgcccgagg ccgccgccgc gacagcagcg cccgcgaaga aggcgaaggc cgcaaccgcg
     1261 acggcagagc cggcagcaga ggtcgccgcc gaggcgccgg ccgctgttga ggagccggct
     1321 gccgaggttg ccgaagagac cgaggaaacc gccgagccag agacccaggc ctagcatccg
     1381 acgagcggtc tgttacgatt cagatcaggg cacgtctact ggctaccggc ctgttcggta
     1441 gccagtctgc tatccgcgac atccgggtag tcgatccggc agccagccgc gacagcgctg
     1501 ctaccgcccg cagactccgc accgagatga ggagcccggc acatcatgag ttctgaagac
     1561 gcagacaacc tcaccgacga gcgcgacgac ctcgacgatg tcgacgacgt cgatgagtat
     1621 gacgatgact acgacgaata cgatgacgac gaagacgtgg acgacgagga cgacgaggac
     1681 ggccagccgg ccgggaccgg cgtcgcgctg accggcgacg cgccgatcga tgaactggcg
     1741 actttcgtcg agttcatggt cagcaacctg gtcgaggagc cggacgactt ccgcgtcgtt
     1801 cccgaacggc tcggcaccaa cgtccacatc aaggtcatcg tgccggaaga ggagatgggg
     1861 cgtgtcatcg ggcgccaggg ccggatcgcc cgctcgatgc gcacgctgct gacaatcgcc
     1921 gcctcccgca aggggctgcg cgccagtctc gacattgact cgtagccgat tcgccgtcgt
     1981 ctcgtgaccg gcatcgagga tccatgaccg acaccccaga cgccggcacg cccactcgcc
     2041 cccgtacccc ctggcgtgag gccaggccgc ccggcccaac caatcccgac ccgaacaccc
     2101 cgccggcaga ggtcttcttc gccgtcgggc ggatcgtcgg cgcccacggc attcgcggcg
     2161 aggcgaagat ggagatcttc accgacgacc ccgaccacct gctcgacctg cgccgcgtct
     2221 acttcgacga tgacccaacc ccgcgcaggc tcagcggcgt ccgtttccac gcccgtcagg
     2281 cgctgctgac cttccccgac atcaccgacc gcgacgccgc cgatgccttg cgcggcacga
     2341 tcgtccgcat ctccggcagc caggcgaagc cgccgaacga gggcgagttc taccattacc
     2401 agctgatcgg tctcgccgtc caggacgagg ccggcaaccc gctcggcacg ctgaccgaga
     2461 tcctcgaagc cggcgaggtc gacacctacg tcgtccgcaa tgaagacggc cgcgaacaac
     2521 tcttcccggc cctcaaggac gtggtgctcg atatcaaccc agccgagaac cgcgtcgtcg
     2581 ttcgcccgct catctgggag gcagatactc cccgcaagcc acacaacccc gaccgaccgc
     2641 cacgaccacg caagctgcga cgcccgaaag ccgggccgtc tacgacctga accgcatctc
     2701 gaataacaat ctgagtagac aaccactgcc gtcttgacgc catgacgagg tgaggatacc
     2761 cgtgtaccct ctgtcgtcct gagccgcagc gaaggacctc cccgtcttgt ccaagcatgt
     2821 cgcagac
//
LOCUS       DASUGJ010000809         4629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1520447, whole genome shotgun sequence.
ACCESSION   DASUGJ010000809 DASUGJ010000000
VERSION     DASUGJ010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520447"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="VFV93_18240"
     CDS             complement(<1..1367)
                     /locus_tag="VFV93_18240"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5167354.1"
                     /translation="MKTVVIGGGLLGLGTGYELAKAGHHVTVLEAGDRLGGLATDVEV
                     AGITVDRFYHCILNSDTHLMNLIDEVGLSDEMRMHPVKAGFLHDGRVHSISSPLDLLR
                     FPPLRPLDRLRLVRSLFACKRVKDWEQLEEVDVETWLRGLSGDRVWDTVWRPLLSAKF
                     DGNFSSTPATYIWSRTVRMTETRSSGGAKELAGHLVGGYRTLVERLAEKIREHGGEVR
                     TSTPVRRLVTRAGAIEQVVTDDATFDCDAAILTVPLPLAARLLADREINGIEDSDRTA
                     AIDGYRKQVEAIEGYLGVVCVLLLLDRPLSEYYTLYLADSSLPFTAVIESTNLIDPVH
                     VGGKHLVYLPKYVDSDSEIFAMSDLDIRGWFLRELRRIYPDLRDEEIVAAPVFRAPHV
                     EPLHPIGSFGTVPTTATPIDGLVLGSTKHFYPKLNNGDAVLRLATRLATEATSRAPQP
                     AAQIAR"
     gene            complement(1397..1765)
                     /locus_tag="VFV93_18245"
     CDS             complement(1397..1765)
                     /locus_tag="VFV93_18245"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5167355.1"
                     /translation="MTMTLICILLSTLLGVGGQILLKQGMSKMGEQSLAVGGLADIGL
                     RIMTSPWVILGLMVYASGTFFWLIALSRVELSFAYPFASLSYVFILASAWALLGETVS
                     ILRLAGVVTICLGVLIVSQS"
     gene            complement(1777..2985)
                     /gene="wecB"
                     /locus_tag="VFV93_18250"
     CDS             complement(1777..2985)
                     /gene="wecB"
                     /locus_tag="VFV93_18250"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX5167356.1"
                     /translation="MDSRPTVITVLGTRPEIIKCSTLLPRFDAAYEHVLIHTGQHYDE
                     QMDAVFFRELGLRAPDVTLGVGSGSHAGQLAKMLLGIEAVLIGRKPDLVFVQGDTNST
                     MAGGLAAAKLNVPVAHLEAGCRSFNRAMPEEVNRVVVDHLAELLLTPDEIAAGHLGDE
                     GIAPERIVNVGSTAIDACLRIAGMPASDGALPGLDTGEREYLVATIHRAENTTPERLR
                     GLFAALADLSTTWPVIVPVHPRTARAIDGLAVPPSVRLIEPVGYATMMRLVGGCRALL
                     TDSGGLQEEAAVLGAPTFILRRETEWRAFVDAGHHQLVGTDPATIVEAVRSTLTGGER
                     ERRMRQPIGHERAGATERVMTALDRYFATGGAPDQPGLEGNSTWNQYTATLSNGHVAV
                     ASAGAQLSQE"
     gene            complement(2978..3766)
                     /locus_tag="VFV93_18255"
     CDS             complement(2978..3766)
                     /locus_tag="VFV93_18255"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5167357.1"
                     /translation="MTQRQASGLSVIVPCCNEEDAVRLLPEKLVPALDQLALDRPVEL
                     VLVDDGSRDQTWSELTRLERTRQAFTIALVRHETNKGLGAALGSGFLASSHPIVVTLD
                     ADGTYPFSIVELLVAKIDAGADIATASPYHPRGGVEGVSALRLTFSRGASVCYRILVD
                     RHVHTWTAMVRAYRAEVLSKAISPETGFLHVAMTLVEARRRGASITEVPAILSTRQVG
                     QSKAKVARITRAHLRYMSGLLWLKLTRRFWIVPRMTQTTVVSHG"
     gene            complement(3767..>4629)
                     /locus_tag="VFV93_18260"
     CDS             complement(3767..>4629)
                     /locus_tag="VFV93_18260"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5167358.1"
                     /translation="IDRAADVTERFGGAIFRQMTDLLEAVDAVSIVSPTTTHAAVAHR
                     ALDFGLHLLVEKPMTASVAEAHCLVDRANEIGRVLLVGHVERFNPTVLELQRLIADKR
                     VRRVALRRIAPFNNRCLDTDVVSDLMIHDIDLVRSLFGSHIRSIAAQGEAVCTEHIDQ
                     ALVELTLTDGPQVSLIASRVGELHTREIDVTMEGSCVLADLLHRTITITSVESPAGRS
                     VTGQYTVPPAEPLRLELQHFLDCIDGRETPRIDADAGLQAIEWVERIDNLIRHRNATQ
                     LAEVQVGGLH"
BASE COUNT          748 a         1461 c         1648 g          772 t
ORIGIN      
        1 cgggcgatct gcgcggctgg ttgaggtgcc cggctggtgg cttcggtggc cagccgggtg
       61 gcgaggcgga ggacggcgtc gccgttgttg agcttgggat agaagtgctt ggttgagccg
      121 aggacgaggc cgtcgattgg cgttgcggtg gttgggacgg tgccgaacga gccgatcggg
      181 tggagtggct cgacgtgcgg ggcgcggaag accggcgcgg cgacgatctc ctcgtcgcgc
      241 aggtcggggt agatccggcg cagctcgcgc aggaaccagc cgcgaatgtc gaggtcactc
      301 atcgcgaaga tttcgctgtc cgaatcgacg tacttcggca ggtagacgag gtgcttgccg
      361 ccgacgtgga ccgggtcgat caggttggtc gattcgatga cggccgtgaa gggcaggctg
      421 ctgtcggcca ggtagagcgt gtagtactcg ctcagcgggc gatccagcag cagcaggacg
      481 cagacgacac ccaggtagcc ctcgatcgct tcgacctgct tacggtagcc gtcgatggcg
      541 gccgtccggt cgctgtcctc gatgccgttg atctcacggt ctgccaggag tcgtgctgcc
      601 agcggtagcg ggacggtcag gatcgccgcg tcgcagtcga aggtcgcgtc gtcggtgacg
      661 acctgctcga tcgcgccggc gcgggtgacg agccggcgga ccggtgtgct ggtgcggacc
      721 tcgccgccgt gctcgcggat cttctcggcc agccgctcga cgagcgtccg gtagccgccg
      781 accaggtggc cggcaagctc cttggcgccg ccggaggagc gcgtttcagt catccggacg
      841 gtgcgggacc agatgtaggt ggcgggagtg ctggagaagt tgccgtcgaa cttggcagag
      901 agcagcgggc gccagacggt gtcccagaca cggtcgccac tcaggccgcg cagccaggtc
      961 tcgacatcga cctcctccag ctgctcccag tccttgaccc gcttgcaggc gaacagcgag
     1021 cggacgaggc gcagccggtc gagtggccgc agcggcggga aacgcagcag atcgagaggt
     1081 gaggagatcg agtggacgcg gccgtcgtgc aggaatccgg ccttgaccgg gtgcatccgc
     1141 atctcgtcgc tgaggccgac ttcatcgatc aggttcatca aatgggtgtc gctgttgagg
     1201 atgcagtggt agaagcggtc gacggtgatg ccggccactt ccacatcggt cgccagcccg
     1261 ccgaggcggt ctccggcttc gaggacggtg acgtggtggc ccgctttcgc cagttcgtag
     1321 ccggtaccga ggccgagcag gccaccgccg atgacgaccg tcttcatcct ggaccttcct
     1381 gtttctattg agccagtcac gattgcgaca cgatcaggac gccgagacag atcgtcacga
     1441 caccggccag ccggaggatg ctgacggtct caccgagcag cgcccaggcg gaggcgagga
     1501 tgaagacgta gctgaggctg gcgaacggat aggcgaacga cagctcgact cgcgagaggg
     1561 cgatcagcca gaagaaggtg ccggaggcgt agaccatcag gccaaggatg acccaggggg
     1621 aggtcatgat ccgcaggccg atatcggcca gcccgccgac cgccagcgat tgctctccca
     1681 tcttcgacat gccctgcttg agcaggatct ggccgccgac gcccagcagc gtgctgagca
     1741 gaatacagat cagggtcatt gtcattggtt tactctctac tcctgcgaca gctgagcgcc
     1801 ggccgaagcg acggccacgt gtccgttgct caacgttgct gtgtactgat tccacgtgct
     1861 gtttccttcc agccccggct gatccggcgc gccgccggtc gcaaaatagc ggtccagcgc
     1921 ggtcatgacg cgctcggtcg cgccggctcg ctcgtggccg atcggttggc gcatgcggcg
     1981 ctcgcgctcg ccgccggtca gcgtcgagcg gacggcttcg acgatcgtgg ccgggtcggt
     2041 cccgaccagc tggtggtggc cggcgtcgac gaacgcgcgc cactcggtct cccggcggag
     2101 gatgaaggtc ggcgcgccga ggacggccgc ctcctcctgg agcccgcccg aatcggtcag
     2161 cagcgcccgg cagccgccta ccagccgcat catcgtggcg tagccgacag gctcgatcag
     2221 ccggacgctg ggcgggacgg ccagcccatc gatcgcccgc gccgttcgcg ggtggaccgg
     2281 cacgatcacc ggccaggtcg tcgagaggtc tgccagcgcg gcgaagagac cccgcagccg
     2341 ctccggcgtc gtgttttcgg cccggtggat cgtcgcgacc aggtactcgc gctcgccggt
     2401 gtcgagaccg ggcaacgcgc catccgacgc cggcatgccc gcaatccgca ggcacgcgtc
     2461 gatagccgtt gagccgacgt tgacgatccg ttcaggggcg atgccctcgt cgccgagatg
     2521 accggcggcg atctcgtccg gcgtcagcag cagctctgcc agatgatcga cgacgacgcg
     2581 gttgacctcc tcgggcatcg cgcggttgaa cgagcgacag ccggcttcga ggtgggcgac
     2641 cgggacgttc agcttggcgg cagccaggcc gccggccatg gtcgagttgg tgtcgccctg
     2701 gacgaagacg agatccggct tgcggccgat caggacggcc tcgatcccta gcagcatctt
     2761 cgccaactga ccggcgtggc tgccggagcc gacgccgagc gtgacatccg gcgcccgtag
     2821 gccaagctcg cggaagaaga cggcgtccat ctgctcgtcg tagtgctgac cggtgtggat
     2881 caggacgtgc tcgtaggccg cgtcgaagcg gggcagcagt gtcgagcact tgatgatctc
     2941 cggccgggtg ccgaggacgg tgatgacggt tgggcggcta tccatggctg accaccgtcg
     3001 tctgcgtcat gcgcggcacg atccagaacc ggcgcgtgag cttgagccag agcaggccgg
     3061 acatgtagcg caggtgggcg cgggtgatgc gggccacctt cgccttcgac tggccgacct
     3121 ggcgcgtcga gaggattgcc ggaacttcgg tgatgctcgc gccccgccgc cgcgcttcga
     3181 ccagcgtcat cgcgacatgc aggaagcccg tctctggcga gatcgccttc gagagcacct
     3241 ccgcgcgata ggcgcggacc atcgccgtcc aggtatggac gtggcggtcg accaggatcc
     3301 ggtagcagac cgaggctccc cggctgaagg tcagccgcag ggccgagacg ccctcgacgc
     3361 cgccgcgcgg atgatagggc gaggcggttg cgatgtcggc gccggcgtcg atcttcgcga
     3421 ccagcaactc gacgatcgag aagggataag tgccatcggc gtcgagtgtg acgacgatcg
     3481 gatggctgga ggcgagaaac ccgctcccca gtgcggcgcc gaggcccttg ttcgtctcgt
     3541 ggcggacgag cgcgatcgtg aacgcctgtc tggtccgttc cagtcgggtc agctccgacc
     3601 aggtctggtc gcggctgccg tcatcgacga ggaccagctc gaccggacgg tcgagcgcga
     3661 gctggtcgag cgccgggacg agcttctccg gcaggagccg gacggcgtct tcctcgttgc
     3721 agcagggtac gatgacagag aggccgcttg cctgccgttg tgtcatctag tgcaatcctc
     3781 ctacctggac ttccgccagt tgcgtggcgt tgcggtgcct gatgaggttg tcgatccgct
     3841 cgacccactc gatcgcttgc agcccggcgt cggcatcgat ccggggtgtt tcccggccgt
     3901 cgatgcaatc caggaagtgc tggagctcca gccgcaacgg ctccgcgggc gggacggtgt
     3961 actgaccggt cacactgcga ccagccggag attccacgct cgtgatcgtg atcgtgcggt
     4021 ggagcaggtc ggccagcacg caactgcctt ccatcgtgac gtcgatctcg cgtgtgtgca
     4081 gttcgccaac ccgcgaggcg atcagcgaca cctgcggccc atcggtcagt gtcagttcga
     4141 ccagcgcctg gtcgatgtgc tcggtgcaga cggcttcgcc ctgggctgcg atcgaccgga
     4201 tgtggctgcc gaacaggctg cggacgaggt cgatgtcgtg gatcatcagg tcgctgacga
     4261 catccgtgtc gaggcagcgg ttattgaacg gcgcgatgcg acgcagcgcg acccggcgaa
     4321 cgcgtttatc ggcgatgagc cgttgcaact ctaggacggt tgggttgaac cgttcgacat
     4381 ggccgaccag caggacgcgg ccgatctcgt tcgcgcgatc gacaagacaa tgtgcctccg
     4441 ccacgctggc tgtcatcggc ttctcgacca gcaggtgcag gccgaagtcc agcgcgcgat
     4501 gggcgacggc ggcgtgggtt gtcgttggcg agacgatgct gacggcgtcg accgcctcca
     4561 gcaagtccgt catctgccgg aaaatagcgc cgccgaagcg ctcggtcaca tccgccgccc
     4621 ggtcgatat
//
LOCUS       DASUGJ010000810         6663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1520537, whole genome shotgun sequence.
ACCESSION   DASUGJ010000810 DASUGJ010000000
VERSION     DASUGJ010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520537"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3094
                     /locus_tag="VFV93_18265"
     CDS             <1..3094
                     /locus_tag="VFV93_18265"
                     /inference="COORDINATES: protein motif:HMM:NF037085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX5167359.1"
                     /translation="QQNVVDWLAAASKRQSVLAADYAGVEYAKWAGLDVTGFENLLAT
                     NPSKDAVSAYLGSNVNNPTGYCGYRSPAPHESDYTGYLDQTCFTPCTNVLGCPAPTPT
                     YDQLVKWGAAIANYEFYNRPEFQQASRDIAIGVGFGVGALVTGLALSGGLIGFVGSST
                     TWLALFPFSLAGSLAASIAGAVLFVVVAIVIAVIATINLFNALALPDQLATLISETRA
                     ATPDLAAMLDDPEQAKGLYSLFVGATLPKPRLATCDNAIGGGGFVPCLNRTPIPDANN
                     TDPVFAIQESGTTATTHSNTITWKDTVEQTTRTARVSNTWFIGSESGSSAGTQSLRIH
                     YTDWNGVGQTAWLLRDPIAGYQFLGVADATASSSAMNPDTCIEDGSCSVGSTIKFIGA
                     DDKQYSASLILAAPTVAPTTSADPVVGIPVTLSAIGASPLGLPVTYQWQFEVPGSFCI
                     VSPTCPDGTVYGGDVSGAEVSHTWESSGANKVKLTATDSLGRVTIHTFTVTVASAGAP
                     VLALSSTDVRTGGADQPISLTGVVTHLGATDSVEVAVDWGDGSPSNFARQGSFPSTDI
                     CACISLVSVAGSDPHQYALSATHRYQQPGTYTVTVRVQDPGGQDTEQAIYVIQNQPVI
                     EWPTPDPITYGTPLSAAQLNASVSAPGAIEITGTFKYWYSYPAFPATIGDVPGVRDAP
                     MMVEFFPDDSSYSIPTPVFAQVVVTPADLTITAPSASVRAGATSVPDLTAVAYDGFVN
                     SEGPDVLGGSLTCSLTDDEGNSVTLAPELPRGSYTISCRGLTSSNYEITFVDGTLTVV
                     DSSAPIITPSVVGELGSNGWYTSDVNITWAVSDPESGVTTSTGCEPTLVTEDTTGTSY
                     TCQADSEGGRQTWTVTIHRDVTNPIVSFTGNQGTYGILDTVTITCTATDATSGIATDT
                     CTDVAGAAMFFTPGQHTVAAVATDLAGHTATGETTFVVTATHSDLATLTTQKITHRAT
                     ARLAAGLLQTAERLADLGLNIQSRAVLITYRAAVNVAQRFGYVSSNDATMLINWSRTL
                     "
     gene            3360..5468
                     /locus_tag="VFV93_18270"
     CDS             3360..5468
                     /locus_tag="VFV93_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5167360.1"
                     /translation="MSIDFSYTPRNRYSRWDGTQKIEGFSADDIMDAISDDMFADGDL
                     QRALQRLFRWGMDKPDGDQMPGIRNLMDRLKARRQQELNRYDLGSVLEDLKQRLDEIQ
                     QLERDGIQRRIDEGREKLSQQQSQQAQQQDGQDGESQPSGGEEGDADHTQGLQDMLEK
                     MAARKFQALNQLPDDAAGQIKALTEYEFMDPEARQKFQELLEQLQQQVLQQTFQGMQQ
                     SLQNMTPEDMQDMQNMLSELNEMLEEKARGGEPDFDRFMHKYGHYFGKGINSLEELMD
                     RMAQQMAAMQSLMDSMTPEQRAELWGAMQAVMNDPGVQQEMNRLAENLGQMMPDNPYA
                     QQFRFGGNEDLSLDEALRLMDRLHEIDDLEDQLRGVRDWRDLEHVDDQKIRDLLGDDF
                     QHDLDQLRQLTKILEDAGYIQKTRRGYEMTPRGVRKIGQKALTDIFQHLSRDRFGNHA
                     IDTQGAGGERTDVSKPYEFGDPFLLDLPKTLMNSVQREGVGSPVTLHASDFEVYRTEM
                     ITQSSTVLMVDMSRSMLYNGCFSAAKKVALALDSLIRTQFPRDNLYILGFSYVATRLE
                     PAQLPSISWDEYNYGTNMQHGFMLSRQLLARHKGGNKQVIVITDGEPTAHFDDGQVRF
                     SYPPTYQTLQETLKEVMRCTRDGVTINTFMLERSPYMAGFVSEMAKINKGRAFFATPD
                     RLGEYILVDYVSNKRKLEKA"
     gene            5560..6663
                     /locus_tag="VFV93_18275"
     CDS             5560..6663
                     /locus_tag="VFV93_18275"
                     /inference="COORDINATES: protein motif:HMM:NF024626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEX5167361.1"
                     /translation="MSPPFTPGLVLSRRFYEEAVRPILNEHVPDLAYAAALIGPGSEV
                     LGFDTPISTDHHWGPRVMLFLSERDAPLAASIDDALSRQLPPSFLGYSTNFGPPDEVG
                     VRLLQVTESGPIAHRVEIRTVAGWFTDYLGWDTAARPSIVQWLTFPQHRLCAATAGEV
                     FRDDTGELTSAREALVWYPHDLWLYLLAAQWNRIAEEEAFMARTGDVGDELGSKLVAA
                     RIVKDIMRLCFLMERRYPPYAKWFGRAFRLLKFGTELEPFLLATIDGATWRERERALS
                     IVLERLAAAHNELGITNALDHRVQQYYGRPYRVIGADRFASAIEAHIVDDAVRSLPAR
                     FGSLNQLIDSTDTIESATTRVRLASIYHEPAGR"
BASE COUNT         1333 a         2192 c         1962 g         1176 t
ORIGIN      
        1 ccagcagaat gtggtcgact ggctggcggc cgcgtccaag cgacagtccg tccttgccgc
       61 cgactatgcc ggggttgagt acgccaagtg ggcgggcctg gacgtgacag ggttcgagaa
      121 tctcctggca accaatccca gcaaggacgc agtgtcggca tacctgggca gcaacgtcaa
      181 caacccgacc ggctactgcg ggtaccggtc gccagcaccg catgagtcag actacaccgg
      241 ctaccttgat cagacgtgct tcactccgtg cacgaacgtc cttggttgtc cagcaccgac
      301 gccgacgtac gatcaactcg tcaagtgggg agcagccatt gctaactacg agttctacaa
      361 ccgacctgaa ttccagcagg cctcgcgtga catcgcgatc ggcgtgggct tcggcgtcgg
      421 ggcgttggtt accgggttgg cattgagcgg tggcctgatc ggatttgtcg gaagttccac
      481 cacatggctg gcgctcttcc ccttctcgct ggccggctcc ctcgccgcgt cgatcgccgg
      541 cgcggtgctg ttcgtcgttg tcgccatcgt tatcgccgtc atcgcgacca tcaacctgtt
      601 caatgctctg gcgcttccgg accaactcgc caccctgatc tccgagacac gcgctgcgac
      661 tcctgacctt gcagcaatgc ttgatgatcc ggagcaggcg aagggtctct actcgctgtt
      721 cgtcggtgcg acgctgccca agccgcgcct ggcaacatgc gataacgcga tcggcggcgg
      781 gggcttcgtt ccgtgtctta accggacgcc gataccggac gccaacaaca ccgatccagt
      841 gttcgcgatc caggagagcg gcacgacggc gacgacgcat tccaacacga tcacctggaa
      901 agacacggta gagcagacga ccagaacggc ccgcgtgagc aacacgtggt tcatcggcag
      961 tgagagcggg tcctccgctg gaacgcagtc gctgcgcatc cactacacag actggaatgg
     1021 cgtcgggcag accgcatggc tgctcaggga cccaattgcc ggctaccagt tcctgggggt
     1081 cgcggatgct accgcgtcga gttcagcgat gaatccggat acctgcatcg aagacgggag
     1141 ttgctccgtc ggatccacca tcaagttcat cggcgccgac gacaagcagt actcagcctc
     1201 cttgatcctc gccgccccga cggttgcacc gaccaccagc gccgacccgg ttgtcggtat
     1261 cccggtgacc ctcagcgcca ttggcgcttc gccgctcggg cttccggtca cctaccagtg
     1321 gcagttcgag gttccaggca gcttctgcat cgttagcccg acctgccccg acggcactgt
     1381 ctacggcgga gatgtctcgg gcgccgaagt gagtcacact tgggagagca gcggcgcaaa
     1441 caaggtgaag ctcacggcca ccgactccct gggacgagtg acgatccata ccttcacggt
     1501 caccgtcgcc agtgccggag cgccggtcct cgcactctcc tcaaccgatg ttcggactgg
     1561 tggcgctgat caaccgatat ccctgaccgg tgtcgttacg catctgggcg ccactgactc
     1621 ggtcgaggtc gcggttgact ggggagacgg ctcaccatcc aacttcgcaa ggcaaggatc
     1681 cttcccgagc accgatatct gcgcatgcat cagcctcgtc agcgtcgccg ggtctgatcc
     1741 gcatcaatac gcgctcagtg cgacacatcg ataccagcaa cccggcacct acacggtgac
     1801 ggtacgggtt caggatccgg gtgggcaaga tacggagcag gctatctacg tcatccagaa
     1861 ccaaccagtc atcgaatggc caacgcccga tcccattacc tatgggacac cgttgagtgc
     1921 cgcccaactc aatgcctccg tctcggcacc tggagccatc gaaatcaccg gcacattcaa
     1981 gtactggtac tcctacccgg cgtttcccgc caccattggc gatgtcccgg gagtcagaga
     2041 tgctccgatg atggtggagt tctttcccga tgacagcagc tacagcattc caacacccgt
     2101 tttcgctcag gtggtggtca caccggcgga cctgaccatc accgcaccaa gcgcgtcggt
     2161 gcgggccgga gcgacgtctg tgcctgacct cacggccgtc gcatacgacg ggttcgtgaa
     2221 cagtgagggc ccagacgtcc tcggcggcag cctgacgtgc tcgctgacgg acgacgaagg
     2281 caattcggtc acgctggctc ccgaactacc tcggggcagc tacaccatct cctgccgggg
     2341 attgacgtcg agtaactacg agatcacgtt cgtggacggc acactgacgg tcgttgacag
     2401 cagcgcgccg atcatcaccc cgtccgtcgt gggtgagctc ggatcgaacg gctggtacac
     2461 cagcgatgtc aacatcacct gggccgtctc cgacccggag tcaggcgtca ccacatctac
     2521 tggctgcgag ccgacgctcg tgaccgagga cacgaccggc acgagctata cctgccaggc
     2581 ggacagcgaa ggcggccgcc agacctggac ggtcaccatc catcgcgacg tgacgaaccc
     2641 aatcgtctcg ttcaccggca accagggcac gtacggaatc ctcgatacgg tcacgattac
     2701 ctgcacagcc accgacgcga cttccggtat cgccaccgac acgtgcacgg atgtcgccgg
     2761 agcggccatg ttcttcacgc cgggccagca caccgtcgcg gcggtggcca ccgacctggc
     2821 cggccacacc gcaacaggcg aaacgacctt cgtggtcacc gcgacacata gcgatttggc
     2881 cacgttgacc acacagaaga tcacgcatcg ggcgacggcc cggctggcag ccggcctgct
     2941 ccaaaccgcc gaacgcctgg ccgacctggg gttgaacatc cagtctcgtg cggtcctgat
     3001 cacctatcgg gcagccgtga atgtcgcgca acggttcggc tatgtgtcga gcaatgacgc
     3061 aacgatgctg atcaactggt cacgcacgct ctgacacttg ggggacggtc gagggatccc
     3121 tgataacaac agcgcagcag ccttctggct gctgcgctgt tttgtcgaat ctcacggtcg
     3181 ttgcacctct gtcgcaacgg gactatgctt atctggctgc caagctcatc gatcgatcgc
     3241 aacgacacag cactgcgtct cagccacctc cgatcggtct caccaaccaa cgaccatgtc
     3301 atctcgatcg tgatgtggca cgagcgacct cagtccgata acgaattgag cgcgacacaa
     3361 tgagcatcga cttttcctac acaccacgca accgctactc ccgctgggac ggcacccaaa
     3421 agatcgaggg cttctcggcc gacgacatta tggatgccat ctccgacgat atgttcgcgg
     3481 atggcgacct tcagcgcgcc ctccagcgtc tgttccgctg gggtatggac aagccggatg
     3541 gcgaccagat gccgggcatc cgcaacctga tggaccgtct caaggcgcgt cgccagcagg
     3601 aactgaaccg ctacgacctc ggctcggtcc tcgaagatct caagcagcgg ctggacgaga
     3661 tccagcaact cgaacgggac ggcattcagc gtcggatcga tgagggccgc gagaagctgt
     3721 cccaacaaca gtcccagcaa gcgcagcagc aggatggcca ggacggcgaa tcccagccgt
     3781 cgggcggcga ggaaggtgat gccgaccata cccagggctt gcaggacatg ctggagaaga
     3841 tggcagcccg caagttccag gcgctcaacc agctacccga cgacgcggcc ggccagatca
     3901 aggcgctgac cgaatacgag ttcatggacc cagaggcacg ccagaagttc caggaactgc
     3961 tcgaacagct ccagcagcag gtcctgcagc agaccttcca gggtatgcag caatcgctcc
     4021 agaacatgac tcccgaagac atgcaggaca tgcagaacat gctttcggag ctgaacgaga
     4081 tgctcgaaga gaaggcgcgc ggcggcgagc cggacttcga ccgctttatg cacaagtatg
     4141 ggcactactt cggcaagggg atcaactcac tcgaagagct gatggaccgg atggcccagc
     4201 agatggccgc catgcagtcg ctgatggact cgatgacgcc cgagcaacgc gccgagctgt
     4261 ggggcgcgat gcaggccgtc atgaacgacc ccggcgtcca gcaggagatg aaccggctgg
     4321 cagagaacct cggccagatg atgccggaca acccctacgc ccagcagttc cgcttcggcg
     4381 gtaacgagga tctctcgctc gacgaggccc tccggctgat ggatcggctg catgagatcg
     4441 acgacctcga ggaccagttg cgtggcgtcc gtgactggcg cgatctggaa catgttgacg
     4501 accagaagat ccgcgacctg ctcggcgacg acttccagca cgacctcgac cagctccgcc
     4561 agttgacgaa gattctcgaa gacgccggct acatccagaa gacccgccgg ggctacgaga
     4621 tgactccgcg tggcgttcgc aagatcggtc agaaggcgct gaccgacatc ttccagcacc
     4681 tcagccgcga ccgcttcggc aaccacgcga tcgacacgca aggtgccggt ggcgagcgga
     4741 ctgacgtctc gaagccgtac gagttcggcg acccgttcct gctcgatctg ccgaagacgc
     4801 tgatgaactc ggtccagcgc gagggtgtcg gctcaccggt gacactgcat gccagcgact
     4861 ttgaggtcta ccggacagag atgatcacgc agtcgtcgac ggtcctgatg gtcgacatga
     4921 gccgctcgat gctctacaac ggctgcttct cggccgccaa gaaggtcgcg ctggcgctgg
     4981 acagcctgat ccggacccag ttcccgcgcg acaacctcta catcctcggc ttctcctacg
     5041 tcgcgaccag gctggagccc gcgcaactgc cctcgatctc gtgggacgaa tacaactacg
     5101 gcaccaacat gcagcacggc ttcatgctct ctcggcaact gctggcgcgc cacaagggcg
     5161 gcaacaagca ggtgatcgtc atcaccgacg gagagccgac ggcccacttc gacgacggcc
     5221 aggtgcgttt ctcgtacccg cctacctacc agacgttgca ggagacgctc aaggaggtca
     5281 tgcgctgcac ccgcgatggc gtgacgatca acacgttcat gctggagcgc agcccatata
     5341 tggccggctt cgtctcggag atggccaaga tcaacaaggg tcgcgccttc ttcgccacgc
     5401 cggaccgcct cggtgagtac atcctcgtcg attacgtctc gaacaaacgc aagctggaga
     5461 aagcgtaggc gtcctgatac gtcagtctga agcgaaccaa cgtaccagag aggacacgtc
     5521 ctgcgtgtcc tctctggcgt cagagacttg gatgaagtgt tgtcaccacc gttcacgccc
     5581 ggcctcgtcc tcagccgccg gttctacgag gaagcggttc gtcccatcct caacgagcac
     5641 gttcccgacc tggcatacgc agcggcgctg atcggccccg gttccgaggt actcggcttt
     5701 gacacgccga tctcgaccga tcatcactgg ggaccgcgcg tcatgctctt cctgagcgag
     5761 cgcgacgcgc cgctggccgc atcgattgac gacgcactga gccggcagct tccgccctcg
     5821 tttctcggct actctaccaa ctttgggccg ccagacgagg tcggcgtccg gctcctccag
     5881 gtaactgaat cgggtcctat tgcacaccgt gtcgagatcc gaacggtggc gggatggttc
     5941 actgactacc tcggctggga cacggcggct cggccctcga tcgtccaatg gctcacgttc
     6001 ccgcagcacc gcctctgcgc cgccaccgct ggcgaggtct tccgcgacga taccggtgag
     6061 ttgacgtcag cgcgcgaagc gctggtgtgg tatccgcacg acctctggct ctacctgctg
     6121 gcggcgcaat ggaaccgcat cgccgaagag gaagcattca tggcccgcac cggcgacgtc
     6181 ggcgacgagc ttggttcgaa actggtggcg gctcggatcg tcaaggacat catgcggctc
     6241 tgcttcctga tggaacggcg ctacccgccg tacgccaagt ggtttggtcg cgcatttcga
     6301 ttgctgaaat tcggcaccga actggagcca tttctccttg ccactattga tggggctacc
     6361 tggcgcgagc gcgagcgcgc tctgtcaatc gtgctcgaac ggctcgctgc cgctcacaac
     6421 gagcttggca tcactaacgc gctcgatcat cgagtccagc agtattacgg tcgtccctac
     6481 cgcgtcatcg gcgcggaccg attcgcgtcc gcgatcgaag cgcacatcgt tgacgacgct
     6541 gtccgtagcc tgccagccag gtttggctcg ctcaaccagt tgattgactc gactgacacg
     6601 atcgagtcag cgacgacccg ggtacggttg gcgtccatct atcacgagcc agccggtcga
     6661 tag
//
LOCUS       DASUGJ010000811         4299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1243547, whole genome shotgun sequence.
ACCESSION   DASUGJ010000811 DASUGJ010000000
VERSION     DASUGJ010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243547"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1869)
                     /locus_tag="VFV93_18280"
     CDS             complement(67..1869)
                     /locus_tag="VFV93_18280"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5167362.1"
                     /translation="MSDAVTTSTTDNRREFTVEGALVSDRRSPVRWIISHVRRNRLYL
                     SAFIGFTLLASVMTSFTSVLTGQAFDEVLDGDGDRLGIIALSILAVVLVRGVVDLTGR
                     LAVEILGKRLARDARDELYVSLLGKSQTFHNRQRVGDIMARASNDIQQLSMMMTPGVD
                     LILDSTLTIIVPVIFIATINLQLLLAPAIFIVGFAVTLRAYVRRLRPVSGAMRANFGA
                     MNAGLAETVTGIEVVKSTAQEAQEERKFANWATRYRDSFVRQGEVQSLYLPPLVLGLA
                     MTVAFLHGILLVDNGTITIGNLVTFMGLMGMLFFASHISVFTFALVQLGIAGAQRILA
                     IMNETTELDENDGGYRERIRGEIVFENVSFSYGDAPVLRDVSFRVEPGQTVAIVGQTG
                     SGKSTLTKLVNRIYDIDSGRITIDGIDTRDWQLDALRSQVSTIEQDVFLFSKTIAENI
                     GFSLGGRATPEEIERAARDAQAHDFIMSFKDGYETIIGERGVTLSGGQRQRLAIARAL
                     LTDPHILILDDSTSAIDSATEDEIQQAIRRLQEGRTTLLITHRLSQIRWADSILVVHQ
                     GRVIDQGTHDELLTRCDLYRRIFAHYDVAVTADA"
     gene            complement(2119..3000)
                     /locus_tag="VFV93_18285"
     CDS             complement(2119..3000)
                     /locus_tag="VFV93_18285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5167363.1"
                     /translation="MDERDLLAGQFEAHREHLRAVAYRMLGSLSEADDAVQEAWLRLG
                     RADAGEIGNLGGWLTTVVARISLDMLRSRKSRREEPLDSGVSEPVVTTRRGTDPEYEA
                     VMADSVGLAMLVVLETLEPAERLAFVLHDMFALPYDEIAPIVGRTPVATRQLASRARR
                     RVQGADSPSQAGLSGKRKVIDAFLAAARGGDFAAMIAVLDPNIVLRTDRSVSPADTRR
                     EIRGAEAVAGQAMRGGARAARPALINGDVGVVVAPRGRLLMVLNFTVGDNGKVIAIDA
                     IADPDHLSELTLAVIPD"
     gene            3160..3615
                     /locus_tag="VFV93_18290"
     CDS             3160..3615
                     /locus_tag="VFV93_18290"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5167364.1"
                     /translation="MQARMTTPVMVLPDAMKAMMALHASTNGHGISEELHEFVNLRAS
                     QINGCGVCVDMHAKALRKAGVSDERLFGVAAWRDTPYFTDAERAALALTEAVTRIADR
                     PEPVPDEIWDEAARYYDEEALSALIVSIAAINVWNRLNVATRQVAGAWS"
     gene            complement(3575..>4299)
                     /locus_tag="VFV93_18295"
     CDS             complement(3575..>4299)
                     /locus_tag="VFV93_18295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5167365.1"
                     /translation="IREGVTVTGLLRDGERVIGVETDDGPIQAGTTILAAGVWSPLLS
                     ATVGLDLPVVPRRRAIYLTEQTGGLPKRMPFVADFDRGEYIRRDGDRIRISGGFAGND
                     PWDDSFDLASGASDTAWVGDLFPALAGAPLVGGWAGLVELTPDHHPLLGAVPGYDGLV
                     VATGFSGHGVMHAPVTGTLIAELILDGRATTLDIAPLSPLRFAEGRPVATSRLERQTE
                     QGDIVLRPEPSSRRPPPDGSPR"
BASE COUNT          751 a         1406 c         1482 g          660 t
ORIGIN      
        1 gcctcgccct tcgtcggtcg catggagcag ggcgaggctg cggccgtcgc ccccacgcgg
       61 ggggtattac gcatcagctg tcaccgccac gtcgtagtgg gcgaagatgc ggcggtagag
      121 gtcgcagcgg gtcagcagct cgtcgtgggt gccctggtcg atgacgcggc cctgatggac
      181 gacgaggatg ctgtcggccc agcggatctg cgagagccgg tgggtgatga gcagcgtcgt
      241 ccggccctcc tggaggcggc gaatggcctg ctggatctcg tcttcggtgg cggagtcgat
      301 cgcgctggtc gagtcgtcca gaatcaggat gtggggatca gtcagcaacg ccctcgcgat
      361 cgccagccgc tggcgctgac cgccggagag ggtcacgcca cgctcgccga tgatcgtctc
      421 gtagccgtcc ttgaacgaca tgatgaagtc gtgcgcctgg gcgtcgcggg cagcccgctc
      481 gatctcctcc ggcgtcgccc ggccgccgag cgagaagccg atgttctcag cgatcgtctt
      541 cgagaagagg aagacatcct gctcgatcgt cgagacctgc gagcggagcg cgtccagctg
      601 ccagtcacgg gtgtcaatgc cgtcgatcgt gatccggccg ctgtcgatgt cgtagatgcg
      661 gttgacgagc ttggtcagcg tgctcttgcc ggaaccggtc tggccgacga tggcgaccgt
      721 ctggcccggc tcgacccgga aggagacatc gcgcaggaca ggcgcgtcac cgtagctgaa
      781 cgagacgttc tcgaagacga tctcgccgcg aatgcgttcc cggtacccgc cgtcattctc
      841 gtccagctcg gtcgtctcgt tcatgatcgc caggatgcgt tgtgcgccgg cgatgccgag
      901 ctggacgagg gcgaaggtga agacggagat gtgcgacgcg aagaagagca tccccatcag
      961 ccccatgaag gtgacgaggt tgccgatcgt gatcgtgccg ttgtccacca gcaggatgcc
     1021 gtgcaggaag gcgaccgtca tcgccagccc gaggacgagc ggcggcaggt agagcgactg
     1081 gacctcgccc tggcggacga acgagtcgcg gtagcgggtg gcccagttgg cgaacttgcg
     1141 ctcctcctgc gcctcctggg cggtcgactt gacgacctcg attccggtga ccgtctcggc
     1201 cagaccggca ttcatcgcac cgaagttggc ccgcatcgcc ccggagaccg gccgcagccg
     1261 gcggacgtag gcgcgcaatg tgacggcgaa gccgacgatg aagatagccg gcgccagcag
     1321 cagctgcagg ttgatcgtcg cgatgaagat gaccgggacg atgatcgtca gcgtcgagtc
     1381 gaggatcagg tcgacgcccg gcgtcatcat catcgagagc tgctggatgt cgttgctggc
     1441 gcgggccatg atgtcgccaa cgcgctggcg attgtggaac gtctgactct tgccgagcag
     1501 cgagacgtaa agctcgtcgc gggcgtcgcg ggccagccgc ttgccgagga tctcgacggc
     1561 gagccgcccg gtcaggtcga cgacgccgcg cacgaggacg accgccagaa tcgagagcgc
     1621 gatgatgccg agccggtcgc catcaccgtc cagcacctcg tcgaacgcct gcccggtcag
     1681 gaccgacgtg aacgaggtca tgacgctggc cagcagcgtg aagccgatga acgcgctcag
     1741 gtagagcctg ttgcgacgca cgtgggagat gatccagcgt acgggcgagc gccggtcgga
     1801 aaccaaggcg ccttcgacgg tgaactcgcg tcggttgtcg gtcgtcgagg ttgtgaccgc
     1861 gtctgacacg gcgaaccctt ccctgcccac cggcggctgg ccgatgtgga tcaagctggc
     1921 gctcagttgg ccgttgggac ctcggcagca gccgggcgaa cgccgggccg ccaatgcgtc
     1981 gggatcgaag aggccgatcc accgtccttc caaagacgca aggggtatcc tagccggatt
     2041 tcacgccaat ttcaatgggg aaacgacgtc gtcggtacgg cttcttgcgc tggtccgcaa
     2101 cgccggctgt cggcgccatc aatcaggaat gacggccagc gtcagttcgc tgaggtgatc
     2161 ggggtcggcg atcgcgtcga ttgcgatgac tttgccgttg tccccgactg tgaagttcag
     2221 gaccatcagc aaccgaccgc ggggggcgac gacgacgccg acatcgccgt tgatgagggc
     2281 cggccgcgcc gcccgggcac caccccgcat ggcctgaccg gcgacagcct cagcgccgcg
     2341 aatctcccgc ctggtgtcgg ctggtgagac ggagcggtcg gtgcgcagga cgatgttcgg
     2401 gtcgagcacg gcgatcatag cagcgaagtc gccgccacgc gctgccgcga ggaaggcgtc
     2461 gatgaccttc cgcttgccgg agagcccggc ctgtgaggga ctgtccgcgc cctgaacccg
     2521 gcggcgggca cggctggcga gctgccgggt agcgaccggc gttcgcccga cgattggggc
     2581 gatctcgtcg tacggcagcg cgaacatgtc gtgcaggacg aaggccagcc gctcggccgg
     2641 ctccagcgtt tcgaggacga ccagcatcgc cagcccgacc gagtcggcca tgacggcctc
     2701 gtactcggga tcggtgccgc gcctggtcgt gacaaccggc tcggacacgc cagagtcgag
     2761 cggctcctcg cggcgcgact tgcgcgagcg gagcatgtcg aggctgatcc gggcgacgac
     2821 ggtcgtcagc cagccgccga ggttcccgat ctcgccggcg tcggcgcggc ccagccggag
     2881 ccacgcctcc tgaacggcgt cgtccgcctc gctgagcgag ccgagcatcc ggtaggcgac
     2941 ggctcgcaga tgctcgcgat gggcctcgaa ctggccggcc agcagatcac gttcgtccat
     3001 ctgtcacatt cctccactct cacgcgtcat tgtagtaacg ggcggcaaat ggctgacgtg
     3061 acagggagtg aacgatgtaa cggatagccc cactcaacga ctgaggaatg attgttgcag
     3121 cgacgcaaca ggaacacgac ccaacggagg aaacgaccca tgcaggcacg aatgacgacc
     3181 ccggtgatgg tactgcccga cgcgatgaag gcgatgatgg ccttgcacgc atcgacgaac
     3241 gggcacggca tctctgagga gttgcacgag ttcgtcaatc tgcgcgccag ccagatcaac
     3301 ggctgcggcg tctgcgtcga tatgcacgcg aaggcgctgc gcaaggcggg tgtctcggac
     3361 gagcggctgt tcggcgtcgc cgcctggcgc gacacgccct acttcaccga cgccgagcgg
     3421 gcggcgctgg cgctgaccga ggcggtgact cgcatcgccg accggccgga gccggtcccg
     3481 gacgagatct gggacgaggc ggcccgctac tacgatgagg aagcgctctc ggcgctgatc
     3541 gtctcgatcg cggccatcaa cgtctggaac cgcctcaacg tggcgacccg tcaggtggcg
     3601 ggcgcctgga gctaggctct ggccgcagga cgatatcgcc ctgctccgtc tgccgctcca
     3661 gccgcgaggt ggcaaccggc cgcccctcag cgaagcgcaa cggcgagagc ggggcgatat
     3721 cgagcgtggt tgcccggccg tcgagtatca gttcggcgat caacgtcccg gtcaccggcg
     3781 catgcatgac gccgtggccg gagaagccgg tcgcgacgac aagcccgtcg taaccgggga
     3841 ccgcgccgag cagcgggtga tggtcgggcg tcagctcgac cagccccgcc cagccgccaa
     3901 ccaacggcgc accagccagc gccggaaaca ggtcaccaac ccaggccgtg tccgacgccc
     3961 cgctggccag atcgaacgag tcgtcccatg gatcgttccc ggcgaagccg ccggagatgc
     4021 ggattcgatc gccgtcgcgg cggatgtact caccacggtc gaaatcggcc acgaacggca
     4081 tccgcttcgg caagccacca gtctgctcgg tcaggtagat agctcgccgt cgcggcacga
     4141 ccggcaggtc cagcccaacc gtcgcgctca gcagcggcga ccagacaccg gcggccagga
     4201 tcgtcgttcc ggcctggatc ggtccgtcgt ccgtctcaac gccaatgacg cgctcgccat
     4261 ccctcaacaa ccccgtgaca gtgacaccct cacgaatcg
//
LOCUS       DASUGJ010000812         7471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_410532, whole genome shotgun sequence.
ACCESSION   DASUGJ010000812 DASUGJ010000000
VERSION     DASUGJ010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410532"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFV93_18300"
     CDS             <1..360
                     /locus_tag="VFV93_18300"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5167366.1"
                     /translation="VRPEIGDVFTQEAFFRVCSQWGAARISPSVKTPVVSAIPTLILA
                     GQYDPSTPPEFGRIASRTLRNSFFFEFPGVGHYTGNGSPCAHAIMLQFLDDPTQRPDA
                     TCIDQMGPPSWIIPPAP"
     gene            451..2094
                     /locus_tag="VFV93_18305"
     CDS             451..2094
                     /locus_tag="VFV93_18305"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEX5167367.1"
                     /translation="MHRRPLPVVVGLALVFAMVLPVVALAQTGSVSSSLFSLSAPLSG
                     GAEVPTGDPDGFGFARITILRDAGQLCFQTSVARIEPATASHIHAGAGGATGPVVIPL
                     GTPDADGLVRGCVDADAALLTDILTNPWNYYVNVHNAAYPAGAIRGQLSMAGTQPPAP
                     PAPPSWEMETIIEGLNNPRGVDVAADGALHVAEAGSGGDECHDIGTPEEPAEICLGMT
                     GAVTTVADGVVERVGDSLPSLSDRTGTFSGGPHDTATDADGNVYVVTGLGATAADREG
                     LGSDIVSMLGKLYMLAPDGSWSEVADLAAYEFENNTAGDFYVNEETGEIDPSAPNLES
                     NPYSVVAVGDGVLVADAGANAVLHVSAGGTISPFAIFPPEMIESPEFLGLPPGTLIPM
                     DAVPTAITVGPDGAVYVGQLTGFPFPVGDAKIWRLEDLNGDGDAIDEGEMTVYAEGLT
                     AVVDIEFGPDGTLYAVEIARNGLLAAEEAQPDDVEAITGAVVAVAADGSLSEVVSTGL
                     VLPGGVAVAADGSLYVTNGSVFPPGSEMSGSLVHVTHAE"
     gene            2276..2827
                     /locus_tag="VFV93_18310"
     CDS             2276..2827
                     /locus_tag="VFV93_18310"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5167368.1"
                     /translation="MPKERISSRQVFDGRLIRTRVDTVRLPSGREATREVVEHPGAVA
                     ILPVTDAGKLILVRQFRYAVGRSLLEVPAGTREPGEPDEETARRELHEEVGLEAGTIE
                     LLARFFVSPGWCNEELIAYRATELREIGAQPEQDEDLEIVEVDPNDIPGLIAGGEIAD
                     AKTITAVLAHLRDVDERSQPGRS"
     gene            complement(2782..3270)
                     /locus_tag="VFV93_18315"
     CDS             complement(2782..3270)
                     /locus_tag="VFV93_18315"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX5167369.1"
                     /translation="MAADPIRVLFLCTHNSARSQMAEALLARIGGDRFEVFSAGTEAT
                     RVHPLAIRAMAEIGIDISRARSKHLDEFVGKDFDAVITVCDSANESCPVFPWSPQRIH
                     WSFPDPSAVDGAETERYRTFQRVRDAIARHLRTWIASLDDSASISRHERPGCDRSSTS
                     RR"
     gene            complement(3260..4414)
                     /gene="arsB"
                     /locus_tag="VFV93_18320"
     CDS             complement(3260..4414)
                     /gene="arsB"
                     /locus_tag="VFV93_18320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX5167370.1"
                     /translation="MAVTAPSIPFATGSVARRLSVLDRLLPVWIFLAMAIGIGLGKLF
                     PGLSDALDRLKIDTVSLPIAIGLLWMMYPVLAKVKYERIPAAAANRRLLGTSLVLNWV
                     IGPALMFAAAWLMLPDHPGYRTGLILVGLARCIAMVLIWNQLACGDNEYAAVLVALNS
                     VFQIAMYTALGYFYLTVVPGWLGSEGTGLDISVWSIARSVLIFLGVPLLAGFLTRHFL
                     ARAKGDAWYEQRFLPRLGPTALIGLLFTIVMMFALKGEVILDAPLEVGRIALPLLAYF
                     AIMFAVAFGISHLLGFRYGETATLSFTAASNNFELAIAVAIGVFGLASDEALAAVVGP
                     LVEVPVLIGLVYVSLWLGRRLYPDAPELHPTSSPAGVVRSEPEMEVAGGR"
     gene            complement(4512..4877)
                     /locus_tag="VFV93_18325"
     CDS             complement(4512..4877)
                     /locus_tag="VFV93_18325"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5167371.1"
                     /translation="MDEVSVPSAPANEMLDRLGSAFKLLADPTRLRVFDLLMQGVQCN
                     CEIGDALDLSPNLISHHLGALRRAGLVDVERDPNDARWLYYSVNRGALRDVRESIAAF
                     LDADRIHDRHPQCGPASQR"
     gene            5129..6553
                     /locus_tag="VFV93_18330"
     CDS             5129..6553
                     /locus_tag="VFV93_18330"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5167372.1"
                     /translation="MDRSTLPVAVIGAGPVGLAAAAHLVQGGETPVVLEAGSAIGASM
                     RQWSHVQVFSPWRYNVDPVAHGLLTAHGWTMPDEDAYPTGAEILADYLEPFAALPEIR
                     PHVQLNARVVAVTRKGFDKMKSAGRDEAPFVLRIETADGTEREILAKAVIDASGTWET
                     PNPLGVNGVPAIGERALADHIYYGIPDVRVTHRSRYAGKRVLVVGSGHSAFNALNDLI
                     ALRRDEPATEIIWAIRRQAPGQMFGGGENDQLAARGELGRSIERLVSGGQITLVMDFR
                     VAWIGYSDDGIVVASEEQALPTVDEIIATTGFRPNLAMLSELRLDLDPAVESPSILAP
                     LIDPNLHSCGTVRPHGAEELKHPETDFYIAGMKSYGRAPTFLMLTGYEQVRSIVAAIR
                     SDWESARRVELALPETGVCTLGNELATATAISDGCCTTVSPEAALSTTAAGSSCGSPS
                     GCGWPESALATVGAAHSIEGGCCA"
     gene            6611..7459
                     /gene="arsM"
                     /locus_tag="VFV93_18335"
     CDS             6611..7459
                     /gene="arsM"
                     /locus_tag="VFV93_18335"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX5167373.1"
                     /translation="MATTTSQAPEQLRDTVREKYGEVARSVLTLQHTSATSCCSPAET
                     TAGASSCCSGEAQAWGAVNYDATAMADLPEEAVLASLGCGNPIALARLEPGQTVLDLG
                     SGGGIDVLLSATRVGPEGFVYGLDMTDEMLELAERNKAAAGVQNVTFLKGQIENIPLP
                     DNSVDVIISNCVINLSVDKPAVLREAYRVLKPGGQFAVSDVVIQGELASEIRSSVEAW
                     AGCVAGAMEEHEYIDSLTQAGFEGASIEVTAVYNPSDVVSCCANVDSPHDGQIVSGFI
                     RARKPE"
BASE COUNT         1450 a         2440 c         2338 g         1243 t
ORIGIN      
        1 gtgcggccgg agatcggcga cgtgttcacc caggaggcat tcttccgcgt ctgctcgcag
       61 tggggagccg cccgcatctc accaagtgtc aagacgccgg tcgtcagcgc gatcccgacc
      121 ctgatcctgg ccggccaata cgatccgtcc acgccaccgg aattcggacg aatcgccagc
      181 cggacgctgc gcaatagctt cttcttcgaa ttcccaggcg ttggacacta caccggcaac
      241 ggctctccgt gcgcccacgc catcatgttg cagttcctgg acgatcccac acaacgtccc
      301 gacgccacct gcattgacca gatgggaccg ccatcctgga tcatccctcc ggccccctga
      361 ccccgcacca cgctggagaa tctgtcgtta tgaaccgcgt tgtcgggaat gtctcccggc
      421 tagcgagtca atcactgagg gaggtcccag atgcatcgtc gcccccttcc cgtcgtcgtc
      481 ggactagcgc tcgtgttcgc gatggtgctt ccggtcgtgg cactcgcgca gaccggcagc
      541 gtgtccagca gcctcttctc gttgtcggcg ccattgagtg gtggcgccga agttccgacc
      601 ggcgacccgg atggcttcgg cttcgccagg atcacgatcc tgcgcgatgc cggccagctc
      661 tgcttccaga ccagcgtcgc tcggatcgag ccggccacgg cgtcgcacat ccacgccggt
      721 gccggcggcg cgaccggccc ggtcgtcatc ccgctcggga cgcccgatgc cgacggactc
      781 gtgcgcggct gtgtcgatgc cgatgctgcc ctgctgaccg atatcctcac caacccgtgg
      841 aactattacg tcaacgtcca caacgccgcg tatccggccg gcgccattcg cggccaactc
      901 agcatggccg gtactcagcc accagcgccg ccggccccac caagttggga gatggagacc
      961 atcatcgagg gactgaacaa cccacgcggt gtggacgtgg ccgcggacgg cgcgctgcac
     1021 gtcgccgaag ctgggagcgg cggtgacgaa tgtcacgaca ttggtacacc ggaggagcca
     1081 gcggaaatct gcctcgggat gaccggcgcg gtcacgacgg ttgccgatgg tgtcgtcgag
     1141 cgggtcggcg acagcctgcc gtcgttgtcc gaccggaccg gcacgttctc gggcgggccg
     1201 catgacactg cgaccgacgc tgacggcaac gtctatgtcg ttaccgggct tggagcgaca
     1261 gccgcggatc gggaagggct tggcagcgac attgtcagca tgctgggtaa gctctacatg
     1321 ctcgctcccg atggcagctg gagcgaggtt gccgacctcg ccgcctacga gttcgagaac
     1381 aacaccgctg gagatttcta tgtgaacgaa gagacgggcg agattgatcc gtcggcgccg
     1441 aatctcgaga gcaaccccta ctcggtcgtc gcggtcggcg acggcgtcct cgtcgcggat
     1501 gcgggagcga acgccgtcct gcacgtgtcc gccggtggga cgatcagccc ctttgcgatc
     1561 ttcccgcccg agatgatcga gtctcccgag ttcctcggcc tgccgccagg cacgctgata
     1621 ccgatggatg ccgtaccgac cgccatcacg gttggcccgg acggcgctgt ctacgtcgga
     1681 cagctcaccg gattcccgtt cccggtcggc gacgcaaaga tctggcgtct ggaagacctc
     1741 aatggcgatg gtgacgctat cgacgagggt gaaatgacag tctatgccga gggattgacg
     1801 gccgtcgtcg acatcgagtt cggcccggac ggcaccctct acgcggtcga gatcgcccga
     1861 aacgggctgc tggcggctga ggaagcgcag ccggatgatg tcgaggccat caccggcgcg
     1921 gtcgtcgctg ttgccgccga tggctcgcta tcagaagtcg tcagtacggg actcgtcttg
     1981 ccgggcggcg tagctgtcgc tgcggatggg tcgctgtatg tgacgaacgg cagcgtcttc
     2041 cctcccggct cggagatgtc cggctcgctc gtccacgtga cacacgccga ataggcctgc
     2101 acatacacca gcgcaacaga acacggtcgt gatacgccac gaccgtgttc tgttgtcgat
     2161 tcaggaacca tcctcctcca cggtaccttg cccaacacgc ctgtcgcgcc cggccctgtt
     2221 cgcgctaccc ttcgcggcga gagagttacg cgttcgacgt gaagggaacc ctcatatgcc
     2281 gaaagagcgc atctcgtcca gacaggtctt cgatggccgg ttgatcagga cacgggtgga
     2341 tacggtccga ttgccatcgg ggcgggaggc aacgcgcgag gtcgtcgagc atccgggggc
     2401 ggtcgcgatt cttccggtga ccgatgcagg caagctgatc ctcgtccgcc agttccgcta
     2461 cgcggtcggc cgcagcctgc tggaggtgcc ggccggcacc cgcgagccgg gcgagccgga
     2521 cgaggagacc gcccgccgag agctgcatga ggaagtcggc ctcgaagccg gtacgatcga
     2581 gctgctggcg cgcttcttcg tctctcccgg ctggtgcaac gaggagctaa tcgcctaccg
     2641 cgcgacggaa ctgcgggaga tcggcgccca gcccgagcag gacgaggatc ttgagattgt
     2701 cgaggtcgat cccaacgaca tccccggcct gatcgccggc ggcgagattg cagacgccaa
     2761 gacgatcacg gcagtcctcg ctcatctccg tgatgtcgac gagcggtcgc aacctggccg
     2821 ttcatgacga ctgatggagg cggagtcatc cagtgacgcg atccacgttc gcaaatgtct
     2881 cgcgatcgca tcacgaaccc gttggaacgt acgatagcgc tcggtttcgg ccccgtcgac
     2941 ggcgctcgga tcagggaacg accagtggat ccgctgcgga ctccaaggga agaccggaca
     3001 ggactcattc gcgctgtcgc agacagtgat cacggcgtcg aaatccttgc caacgaactc
     3061 gtccagatgc ttcgagcgcg ctcttgagat gtcgatgccg atctcagcca tcgcgcgaat
     3121 cgccagcggg tggacgcggg tcgcctcggt tccggctgag aagacctcga agcgatcacc
     3181 gccgatccgt gcaagcaacg cttccgccat ctgcgatcgt gcggagttgt gagtgcagag
     3241 aaacaggacg cgaatcgggt cagcggccac cagccacctc catctcaggt tccgaccgaa
     3301 cgacgccagc aggagaggac gtgggatgca gctccggcgc atccgggtag agtcgtcgtc
     3361 cgagccagag tgagacgtag acgagcccga tcaggaccgg cacctcgacc aacggcccga
     3421 cgacggcagc cagcgcctca tccgaggcga ggccgaagac accgatcgcc accgcgatcg
     3481 ccaactcgaa gttgttgctg gcggccgtga aggacaacgt cgccgtctcg ccatagcgga
     3541 acccgagcaa gtgcgagatg ccgaacgcga ccgcgaacat gatcgcgaag taggccagca
     3601 gtggcagcgc gatccggccg acttcgagtg gcgcgtcgag aatgacctcg cccttcagcg
     3661 cgaacatcat gacgatcgtg aagagcagcc cgatcagcgc cgtcggaccg agccgtggca
     3721 ggaagcgctg ctcgtaccag gcgtccccct tcgcgcgtgc gaggaagtgc cgcgtcagga
     3781 agccagccag cagcggcaca ccgaggaaga tcaggacgct cctggcgatc gaccagaccg
     3841 agatgtcgag ccccgtcccc tccgagccga gccagcccgg cacgaccgtc aggtagaagt
     3901 agccgagcgc ggtatacatc gcgatctgga agaccgagtt gagcgcgacc agcacggctg
     3961 catattcatt gtcaccgcag gcgagttggt tccagatcag caccatcgcg atacagcgcg
     4021 ccagtccgac gaggatcagg cccgtcctgt agccggggtg atccggcagc atcagccacg
     4081 cggcagcgaa catcagggcc gggccgatca cccagttgag caccagcgat gtcccgagca
     4141 gcctgcggtt cgcagccgcg gccgggatcc gctcgtattt caccttcgcc aggaccgggt
     4201 acatcatcca gagcagcccg atcgcgatcg gcaaggacac ggtatctatc ttgagccgat
     4261 cgagcgcgtc ggagaggccg ggaaagagct tgcccagccc gattccgatc gccatcgcaa
     4321 ggaagatcca gaccggcaac agccgatcaa gaaccgagag tcgcctggcc acactcccag
     4381 tcgcgaaggg tattgacggt gctgttaccg ccatctacgt ctcctccgat catgagtcac
     4441 gagacagata tatcaacact cgttgaacta tcagccgtca gattcgcccc acctgcgaag
     4501 atgcccacct cctatcgctg agaggctgga ccgcactgcg ggtgtcggtc atggatccga
     4561 tcagcgtcca ggaacgctgc aatcgactcg cgcacgtcac gcaatgcgcc acggttaacc
     4621 gagtagtaca gccagcgggc atcgttggga tcgcgctcga catcgaccag gccggcccgc
     4681 cgcaaggcgc cgagatggtg ggagatcaga ttcgggctca gatcgagcgc gtcaccaatc
     4741 tcgcagttgc actgcactcc ctgcatcagc aggtcaaaga cgcgcagccg cgtcggatcg
     4801 gccagcagct tgaaggcgct ccccaaccga tcgagcatct cattcgccgg agccgatggt
     4861 actgagacct cgtccatgac gcgaaactcc agttctttca ggaatagttc aaccgtgatt
     4921 gaaggatact agaataatcc acggcacgtg acaatgtcga cggcgatcag cacccaaccg
     4981 tgtcaacagc gtgccaacca gccacactgg tccaatgggg gcttgacaac gaagtgatcg
     5041 tttcgtaaat tagcgaagcc accgacacgc cggtggatta tctcagccga ttggttcgta
     5101 aaaatacgaa catgattggg gaactcgaat ggatcgttca acactcccgg ttgcggtcat
     5161 cggtgccggt cccgtcggac tggccgctgc tgcccatctg gttcaggggg gcgagacgcc
     5221 ggtcgtcctc gaagcgggct cggcgatcgg cgccagcatg cgccaatgga gccacgtaca
     5281 agtcttctcg ccatggaggt acaacgtcga tccggtcgcg cacggactgc tgacagctca
     5341 cggctggacg atgcctgacg aggatgccta cccgaccggc gctgagatcc tcgcggacta
     5401 cctcgaacca ttcgcggcac tgccagagat tcgcccgcat gtccagttga acgcccgtgt
     5461 cgtcgccgtc acccgcaaag gcttcgacaa gatgaagtcg gccggccggg acgaggcgcc
     5521 cttcgtgctg cggatcgaga ctgctgacgg gaccgagcgc gagattctcg ccaaggcggt
     5581 catcgacgcg tctggaacgt gggaaacccc taatccactc ggcgtcaacg gcgtgccggc
     5641 catcggcgag cgagcgctgg ccgaccacat ctactacggc atccccgatg tccgcgtcac
     5701 acatcgctcc cgctacgcag ggaagcgcgt cctcgtcgtc ggcagcggcc actcggcctt
     5761 caacgcgctc aacgacctca tcgccctccg gcgtgatgaa ccggcaaccg agatcatctg
     5821 ggccatcaga cgccaggcgc cagggcagat gttcggcggc ggcgagaacg accaactcgc
     5881 ggctcgtggt gagcttggtc gcagcatcga gcggctggtc tcaggcggcc agatcactct
     5941 cgtgatggac ttccgcgtcg cgtggatcgg atacagcgac gacggtatcg tcgtcgcgag
     6001 tgaggagcag gctttgccaa ccgtcgacga gatcatcgcg acgaccggct tccggccaaa
     6061 cctggcgatg ctgtccgaac ttcgactcga cctcgacccg gccgtcgaga gtccgagcat
     6121 cctcgcgccg ctgatcgacc cgaacctgca ctcctgcggc accgtccggc cccacggcgc
     6181 cgaggaatta aagcaccccg agaccgactt ctacatcgcc ggcatgaagt cctacggccg
     6241 ggcgccgacc ttcctgatgc tgaccggcta cgagcaggtc cgctcgatcg ttgccgccat
     6301 ccgcagcgac tgggagagcg cccgtcgcgt cgagttggcc ctgccggaga cgggcgtctg
     6361 tacgctgggc aacgagctgg cgacagccac cgcgatcagt gacggatgct gcacgacggt
     6421 atcgcctgaa gcagcactgt ccaccacggc cgccggttcc agttgtggtt cgccgtcagg
     6481 ctgcggatgg ccagagtcag cactggcaac tgttggagcg gcacattcaa tcgaaggagg
     6541 atgctgcgca tagctctcga cgaatcggtt acgatcatca caggcaacct gagacgaaag
     6601 ggatcacgag atggcgacga caacatccca ggcaccagaa caactccgcg acacggttcg
     6661 agagaagtac ggcgaggtcg cccgatcggt actgacgcta cagcacactt ccgccacaag
     6721 ctgctgttcc ccggccgaaa ccacagccgg cgcaagctca tgctgcagcg gcgaagccca
     6781 ggcgtggggc gccgtcaact acgacgccac ggcgatggcg gatcttccgg aagaggcggt
     6841 gcttgcctcg ctcggatgcg gcaatccaat cgcgttggcg cggctcgagc cggggcagac
     6901 ggtgctcgac ctcggatcgg gcggcgggat cgatgtgctg ctttcggcaa cccgcgtcgg
     6961 tccagaaggc ttcgtctacg gcctcgacat gaccgacgag atgttggaac tcgccgaacg
     7021 caacaaagcg gctgccggag ttcagaacgt cacgttcctg aagggccaga tcgagaacat
     7081 tccgttgccg gacaacagcg tcgatgtcat catctccaac tgcgtgatca acctgtcggt
     7141 tgacaagccg gccgtcctgc gagaggcata tcgcgtgctc aagccgggcg gccagttcgc
     7201 ggtctccgat gtcgtcattc agggtgagct ggcgtccgaa atccgctcga gtgtcgaagc
     7261 ctgggccgga tgtgttgccg gagcgatgga ggaacacgag tacatcgaca gcctgacgca
     7321 ggccgggttt gaaggtgctt cgatcgaggt cacggccgtc tacaacccga gtgacgtcgt
     7381 ctcctgctgc gccaacgttg attcacccca cgatggtcaa atcgtctccg gcttcatccg
     7441 ggctcgtaag cccgaatagc agcgggcgga c
//
LOCUS       DASUGJ010000813         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_273116, whole genome shotgun sequence.
ACCESSION   DASUGJ010000813 DASUGJ010000000
VERSION     DASUGJ010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273116"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VFV93_18340"
     CDS             complement(<1..951)
                     /locus_tag="VFV93_18340"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX5167374.1"
                     /translation="MKLEHSTMDPTPTAGSYEAARERAAWLDRRSRGRIVVSGRDRAT
                     YLHSLLTNDIVALKAGAGCYSAYLTPQGRMITDLWVYELGDVILIELGQDTKDTLLAK
                     LDQFVFSEDVQLGDVTEAFGAVAVVGPQASQVVSELLETVTKPALDALPDHGNLRATF
                     GGQPAIVLRVSDTGEPGYEVVIDRAAYDECSARLIAAGIPQLDGATAAAIRIEDGVPL
                     FGQDMDEETIPLEAGIEGRAISLTKGCYVGQEVIIRVLHRGHGRVARHLVGLSIDSDR
                     VPAAGATLTSADKEIGRVTSAAWSPALGRPVGLGYVHRDYA"
     gene            1016..>2307
                     /locus_tag="VFV93_18345"
     CDS             1016..>2307
                     /locus_tag="VFV93_18345"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5167375.1"
                     /translation="MLSTTALVVVLGAQSAPETASSSTFTIYLRSAPIGSETISVART
                     PDGWTISSSGRTGLPIDLATRQLQLHYTPDWKPIDLSIDATIRGVQLSGRTTVSGTTA
                     QSSFLQNGETVNRTDTIAPDAVLLINPLWAPFEALARRVRNAPPGTTVPGHVLGTAFS
                     IEVGPSKDETLQMGTQTFSARRTAVTLPSGGAMLQAEIWSDDAGRLLRVSIPAQNLEV
                     VREDIASVAVRVVKVSRPNDEDIRIPANGFRLAATLSKPMNAAPKDKLPLVVLVGGSG
                     PTDRDETVYGIPIFGQLAGALADAGFMVVRYDKRAVGQSGGRAESATLNDYADDLIAV
                     LTAVGKRDDVDDDRVAVLGHSEGGSVAMIAASREGDIKALALVATMGTTGAELNMWQV
                     MHAMERSKQPPEETATTIELQRRIQEAVITGKGWDRIPA"
BASE COUNT          428 a          752 c          717 g          410 t
ORIGIN      
        1 tgcgtagtcc cgatgcacgt agccgaggcc gacgggacgg ccgagcgcgg gagaccaagc
       61 ggcgctggtg acccggccta tctccttgtc cgccgatgtc aacgtcgctc ctgcagcggg
      121 cacgcgatcg ctgtcaattg acaagccgac caggtggcgc gcaacgcggc cgtgcccgcg
      181 atgcaacact cggataatca cttcctgacc aacgtagcaa cccttggtca ggctaatcgc
      241 ccgtccttcg attcctgcct cgagcggaat cgtttcctcg tccatgtcct ggccgaagag
      301 aggcacgccg tcttctattc ggatcgctgc cgccgtcgca ccgtccagct gcgggatgcc
      361 tgccgcaatc agccgtgcgc tgcattcgtc gtaggcggcg cgatcgatca cgacttcgta
      421 tcccggctcg ccggtgtcgc taactctcag cacgatcgct ggttgaccgc cgaacgtcgc
      481 gcgcagattt ccatgatccg gaagcgcatc caacgccggc ttggtgacgg tctcgagcag
      541 ttccgatacg acctgcgatg cctgagggcc gacaacggca acggcgccga aggcctcggt
      601 gacatcgccc agctgaacgt cttcagaaaa aacgaattga tcgagctttg cgagcaacgt
      661 gtccttggtg tcttgcccga gctcgatgag gatcacatcg ccaagctcat agacccaaag
      721 gtcagtgatc atgcggccct gcggcgtgag gtaggcgctg taacagcccg ccccggcctt
      781 caaggcgacg atgtcgttgg tcagcaggct gtgcagatag gttgcgcggt cgcgcccgga
      841 cacgacgatg cgtcctcgac tccgtcgatc cagccacgcc gctcgctcgc gagccgcctc
      901 atagctgcct gctgtgggcg tcggatccat ggtgctatgc tctaacttca tgcgctcgca
      961 tcattgtacg cgccgcagcg gctgggctct tggagcctcc gccacgctgg cggccgtgct
     1021 gtcgaccaca gcgcttgtcg tcgtccttgg cgcccagtcg gcgccggaaa ccgccagcag
     1081 ctcgacgttc acgatctatc ttcgctcggc gccaatcggc agcgagacga tatctgtcgc
     1141 acgcacgccg gacggctgga ccatcagcag ctctggacgg acaggtcttc ccatcgacct
     1201 tgcaacgcgt cagctgcagc tccactacac accggattgg aagccaatcg atctctcgat
     1261 tgacgccacg attcgtggtg ttcagttgtc gggacggacg accgtgtccg gcaccaccgc
     1321 ccagagctcg tttctgcaaa acggcgaaac agtcaaccgg acggacacga tcgcccccga
     1381 cgcagtcctc ttgatcaacc cgctgtgggc gccgttcgag gcgctggccc gacgagttcg
     1441 caatgcgccg ccgggcacga ccgttcccgg ccacgttctg ggcaccgcat tttccatcga
     1501 ggtcggaccg tcgaaggacg agaccctgca gatgggcacc cagacgtttt cggcacgccg
     1561 aaccgcagtc acgctgccgt cgggcggcgc gatgctccag gcagaaatct ggtctgacga
     1621 tgcgggtcgg ctgctgcgag tcagcattcc tgcgcagaac ctggaggtgg tgcgcgaaga
     1681 catcgcatca gtggccgtac gcgtcgtcaa ggtgtcgcgg ccgaacgacg aagacatcag
     1741 aattccggcg aacggattcc gtctcgcggc gacgctctcg aagccgatga acgcggcgcc
     1801 gaaagacaaa ctgccgctcg tcgtgctggt cggcggatcc gggccgacgg atcgagatga
     1861 aacggtttac ggcattccga tttttggaca gcttgcgggt gcgctcgccg atgcgggatt
     1921 catggtggtg cgctacgaca aacgtgcggt cggccagagc ggcggccgag ccgaatcggc
     1981 aacgctgaac gactacgccg acgatctcat cgcggtcctc acggccgtcg gcaagcgaga
     2041 cgacgtggac gacgaccgtg tggccgtcct cggtcacagc gagggtggct ccgtcgcgat
     2101 gattgccgcc tcgcgcgaag gggacatcaa ggcccttgcg ctcgttgcca cgatgggcac
     2161 gactggagcc gagctcaata tgtggcaggt gatgcacgcc atggagcgat cgaaacagcc
     2221 tccggaagaa actgccacca ccatcgagct gcagcgccgc attcaagaag cggtcataac
     2281 gggtaagggc tgggatcgaa tccctgc
//
LOCUS       DASUGJ010000814         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_688500, whole genome shotgun sequence.
ACCESSION   DASUGJ010000814 DASUGJ010000000
VERSION     DASUGJ010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688500"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFV93_18350"
     CDS             <1..624
                     /locus_tag="VFV93_18350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5167376.1"
                     /translation="CNICKGSHRKLARKMALLSQGPTPPAGFVVEDLVAAGRAPHQSF
                     FHQWRAEDETAIDCALERCGICDLRYREIETLSGGQRQRAWMALALAQETPVLLLDEP
                     TTFLDIAAQIDLLDLAWTLNRDEGRTVVVILHDLNMAARYADLIVALKDGQVIASGTP
                     AEVMNGEVLRTVFGIEATVTDDPLTGAPLVMPIQAAVAETEDALLAG"
     gene            655..1479
                     /locus_tag="VFV93_18355"
     CDS             655..1479
                     /locus_tag="VFV93_18355"
                     /inference="COORDINATES: protein motif:HMM:NF023007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5167377.1"
                     /translation="MELTLTATSTPIAATLARAAGQIEYFSGSYGERPDDWLLASEVI
                     DDPDALSTLLDQVLALYQTDNRQIGAAFLVLGYFWSPMLAMMACFTLDGRVPDLTPDS
                     VAFDLRGGVRFTSPAFHALPSDPATGQPEVTLLPDHDALRDQLVAQLEAHANPLFTTL
                     RAVAPYGLNGMRANYIDRLVSAIIWISEALGDQEIARREVPAFVSRLTTKHRAGIIEV
                     CHEGRVGIYQQRSGCCLNYRLPGKEKCDTCSLHPLEKRLQIFRGLMSDQTSAGPSD"
     gene            1672..2673
                     /locus_tag="VFV93_18360"
     CDS             1672..2673
                     /locus_tag="VFV93_18360"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5167378.1"
                     /translation="MRSWRVWLGFGISLVFLYFAVRGQDFERIGEALREASYIWLVPA
                     LVAYFLGVAVRSIRWHFLLRSVQDIAPRNLFPIVTIGFMANNVLPFRAGEVVRAYALS
                     ARYGVRKSAALATIAIERIFDGMTMLIFMLVASLSIALTSDLRAVAIIAAILFSLLSA
                     MLVLFVFLPGLRDRAIDVSVHFLPERAGSRVQEMAHSFFEGLGILKRRQDLISVGLTS
                     LLAWLLEGSMYLLIIEGFKLHVSPLAILMVTAVANLATLIPSSPGYVGPFETGVLLVL
                     NGALGIERELALSFAIVVHAALYFPITIFGLVLWWRESLSWREVRQSEGVNGVVGDG"
BASE COUNT          418 a          980 c          858 g          494 t
ORIGIN      
        1 tgcaacatct gcaagggctc gcaccgcaag ctggcccgca agatggcgct cctgtcgcag
       61 gggccgacgc caccggccgg cttcgtcgtc gaagacctcg tcgcggccgg gcgggcgccg
      121 caccagtcgt tcttccacca gtggcgcgcc gaagatgaga cggccatcga ctgtgccctg
      181 gagcgctgcg gcatctgcga cctgcgctac cgtgagatcg agaccctttc cggcggacaa
      241 cgccagcggg cctggatggc gctggcgctg gcgcaggaga cgccggtcct gctgctggac
      301 gagccgacga cgttcctcga catcgcggcc cagatcgacc tgctcgacct ggcctggacg
      361 ctcaaccgcg acgaaggacg aacggtcgtc gtcatcctcc acgatctcaa catggctgcc
      421 cgctacgccg acctgatcgt cgccctcaag gacggccagg tgatcgcctc cggcacaccg
      481 gccgaggtga tgaacggcga ggtgctgcgg acggtcttcg ggatcgaggc gaccgtcacc
      541 gacgacccgc tgaccggcgc gccgctcgtg atgccgatcc aggcagccgt cgccgaaaca
      601 gaagacgcgc tgctggccgg ctgaggtccg atcaacaggg gaaacgggga acgtgtggaa
      661 ctgacactga ctgccaccag cacgccgatc gccgcgacgc tggcccgcgc ggccggccag
      721 atcgaatact tctccggcag ctatggcgag cggcccgacg actggttgct ggcgtcggag
      781 gtcatcgacg atcccgacgc gctcagcacg ctgctcgacc aggtgctggc gctctaccag
      841 acggacaacc gccagatcgg cgcggccttc ctcgtcctcg gctacttctg gagtccgatg
      901 ctggcgatga tggcctgctt cacgctcgac gggcgcgtgc cggacctcac gccggattcg
      961 gtcgccttcg acctgcgcgg cggcgtccgc ttcacctcgc cggccttcca cgcgctgccc
     1021 agcgacccgg ccaccggcca gcccgaggtc acgcttctgc ccgaccacga cgccctccgc
     1081 gaccagctcg tcgcgcagct tgaggcgcac gccaacccac tcttcacgac gctgcgggcc
     1141 gtcgccccgt atggcctcaa cggcatgcgc gccaactaca ttgaccggct cgtcagcgcg
     1201 atcatctgga tctccgaagc cctcggcgat caggagatcg cccgccggga ggtccccgcc
     1261 ttcgtcagcc ggctgacgac caagcaccgc gccggcatca tcgaggtctg ccacgaaggt
     1321 cgcgtcggca tctaccagca gcgcagcggc tgctgtctca actaccgcct gcccggcaag
     1381 gagaagtgcg acacctgctc actccacccg ctcgaaaaac ggctgcaaat ttttcgcggg
     1441 ctgatgagcg accaaaccag cgcaggtccc agcgactgat cggtggcaca atcgtcaatc
     1501 cccaatggtc aatccggtcg tccaaccatg agtggtatcc ttcccggctg tgcctgtacc
     1561 gtcccatgac cgacgcgcgg gacgaaatgc agcgtcctga cttatcgttt ccggtggccg
     1621 gccggtggct gtccgccgtg gtctgatcaa tcgtcattgc tccggaggat catgcgctcc
     1681 tggcgcgtct ggctcgggtt cggtatcagc ctcgtcttcc tctacttcgc cgtccgcggc
     1741 caggatttcg agcgcatcgg tgaggcattg cgcgaggcga gctacatctg gctcgtgccg
     1801 gcgctggtcg cctacttcct tggtgtggcg gtccggtcga tccgctggca cttcctgctt
     1861 cgctccgtcc aggacatcgc cccccgcaac ctcttcccga tcgttacgat cggcttcatg
     1921 gccaacaacg tcctgccctt ccgggccggc gaggtcgtcc gcgcctacgc gctctcggcc
     1981 cgctatggtg ttcgcaagag cgccgcgctg gcgacgatcg cgatcgagcg catcttcgac
     2041 ggcatgacga tgctgatctt catgctggtc gcctcgctct cgatcgcgct gaccagcgac
     2101 ctgcgggcgg tcgccatcat cgcggcgatc ctcttctcgc tgctctcggc gatgctggtg
     2161 ctgttcgtct tcctgccggg cctgcgcgac cgggcgatcg acgtctcggt tcacttcctg
     2221 ccggagcggg ccggctcgcg ggtccaggag atggcccact cgttcttcga ggggctcggc
     2281 atcctgaaac gccgccagga cctcatcagc gtcgggctga cctcgctgct ggcctggctg
     2341 ctggaagggt cgatgtatct gctgatcatc gagggcttca agctgcacgt ctcgccgctg
     2401 gcaatcctga tggtgacggc cgttgccaat ctggcgacgc tgatccccag ctcacccggc
     2461 tacgtcggcc cgttcgagac gggcgtcctg ctcgtcctca acggcgcgct cgggatcgag
     2521 cgcgaactgg ccctctcgtt cgccatcgtc gtccacgccg cactctactt ccccatcacc
     2581 atcttcggcc tcgtcctctg gtggcgcgaa agcctctcct ggcgagaggt ccggcagtcg
     2641 gaaggtgtga atggggttgt gggagatgga taaagatcta gatctgaacc ggcccccgtg
     2701 taggggcgat ggccgcagcc tcgccctgca tcgtgcgcct ggcaaggggc
//
LOCUS       DASUGJ010000815         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_827838, whole genome shotgun sequence.
ACCESSION   DASUGJ010000815 DASUGJ010000000
VERSION     DASUGJ010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827838"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1185
                     /locus_tag="VFV93_18365"
     CDS             175..1185
                     /locus_tag="VFV93_18365"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5167379.1"
                     /translation="MRAGRSLRRRLLLACTLVIVAVVTIPVAVGLAVNLGGASVVNTD
                     GDGLNLRDSPSLSGAILTGMPEGAQVEVLETGFTGDGLDWAYLRYDGVEGYAAVTYLS
                     NGNEPVAVPIVQPPSDQPPADTPAPAAGLAVGDRAIVSGTNGDGVNVRSGPGTEYRVV
                     SGLAEGSNVPILGGPSPDADGADWFQVEAWGVSGWVHSDYLAEVGGAPVSGDAVGEAI
                     AAEALKYLGAPYVWAGTNPNGFDCSGFTYFIMNWVLGNDFPRAIEEQLASGEYVHPND
                     LQPGDLVFFTDTYKPGLSHVGIYIGKGEFVNAGSEDDVVAISYMWDDYWGPRYLESRR
                     IR"
     gene            1282..1941
                     /locus_tag="VFV93_18370"
     CDS             1282..1941
                     /locus_tag="VFV93_18370"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167380.1"
                     /translation="MTVVVHERVETIPDEIVEGFKSLGVGDIGHVQVKGFMDTAIRPV
                     WSDIRLVGRAVTVWMPSMDIGVSRAAIRACKPGDVLVVDRGGDTEFACWGGGAGLLAM
                     VQGVAGLIVDGAVTDAMEITDLKWPVYSRTISGLVGHPQREFGEVNTTISCGGVPVSP
                     GDLIVADDDGIVVVRPHEAEALMKEVQDRFGSTPSIRQWLRDGKDIADHPIAQKFFNQ
                     Q"
BASE COUNT          339 a          699 c          691 g          370 t
ORIGIN      
        1 cggcctcacg ccggcccctt gacagtgtat gtactatgtg tacgtacacc tagcgtggcg
       61 cgaacctttc tgcgtcctgc tgggcgtacc gcacctcgga tgcgctgttt tgcggagaaa
      121 tcgcgtctca gcgcccgtcg tgcgcggttt gccggttccc ggaaaggatc gccgatgcgc
      181 gctggacgct cgctccgtcg ccgtctgctg cttgcatgta cgctcgtcat cgttgctgtc
      241 gtcacgatac ccgtcgccgt cggtctggcc gtcaacctgg gcggcgcgtc cgttgtcaac
      301 accgatggcg acggcctcaa cctgcgcgac agcccctcac tgagcggcgc gatcctgacc
      361 ggcatgcccg aaggcgctca ggtcgaggtg ctggagaccg gctttaccgg cgacgggctc
      421 gactgggcct acctgcgcta cgacggggtc gagggctacg ccgccgtcac gtacctcagc
      481 aacggcaacg agccggtcgc cgtcccgatc gtccagccac ccagtgacca gccaccggcc
      541 gacacgccgg caccggccgc cgggctggcc gtcggcgatc gagcaattgt cagtggcacc
      601 aacggcgatg gcgtcaacgt ccgtagcggt cccggcacgg agtacagggt cgtgtccggc
      661 ctggccgaag gctccaacgt cccgatcctt ggcggtccct cgcctgacgc ggacggcgcc
      721 gactggttcc aggtcgaagc ctggggtgtc agcggatggg tgcacagcga ttacctggcc
      781 gaggtcggcg gcgcgccggt ctccggcgac gccgttggcg aggcgatcgc cgccgaggcg
      841 ctcaagtacc tcggcgcgcc ctacgtctgg gccggcacca acccgaacgg cttcgactgc
      901 tccggcttca cctatttcat tatgaactgg gtgctcggaa acgacttccc ccgtgcgatc
      961 gaggaacagc tcgccagcgg cgagtacgtg catcccaacg atcttcaacc cggcgacctc
     1021 gtcttcttca cggacaccta caagcccggc ctctcgcacg tcggcatcta catcggcaag
     1081 ggtgagttcg tcaacgccgg cagcgaggac gatgtcgtcg ccatcagcta catgtgggac
     1141 gactactggg gaccgcgcta cctggaatcg cgccggatcc gctgattgct ggcgggcatg
     1201 atgccgctgg cggcgtctcg tggcatcatg cccgccagga tgtttgcggt tccgagggga
     1261 acgggatgag agggagagac aatgacagta gtcgtgcatg agcgggtcga gacgatcccg
     1321 gacgagatcg tcgagggctt caagtcgctc ggcgtcggcg atatcggcca cgtccaggtc
     1381 aaaggcttca tggacacggc catccggccg gtctggagcg acatccgcct cgtcgggcgg
     1441 gccgtcacgg tctggatgcc gtcgatggat atcggcgtct cgcgggcggc gatccgtgcc
     1501 tgcaagcccg gcgatgtcct ggtcgtcgac cgtggcggcg acaccgagtt cgcctgctgg
     1561 ggcggcgggg ccgggctgct ggcgatggtc cagggcgtgg caggactgat cgtcgatggc
     1621 gccgtcaccg acgcgatgga gatcaccgac ctgaagtggc cggtctactc acggacgatc
     1681 tccggcctgg tcggtcaccc gcagcgggag ttcggcgagg tcaacacgac catcagctgt
     1741 ggcggcgtcc cggtctcgcc cggtgacctg atcgtcgccg acgatgacgg gatcgtcgtc
     1801 gtgcgccccc acgaggccga ggcgctgatg aaggaagtcc aggaccgctt cggcagcacg
     1861 ccgagcatcc gccagtggct gcgcgacggc aaggacatcg ccgaccaccc gatcgcccag
     1921 aagttcttca atcaacaata gagctccgat gacatcccaa ttcgtcccgg ctggaccgct
     1981 tgcctgagcg gttcagcctc gttatgctgt cggtatacga tccgtatgac cgttctccac
     2041 cggaacacat ctcgcattcc gtcgcatctc gtacggactg gcggacaacg ttggcatcg
//
LOCUS       DASUGJ010000816         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1801368, whole genome shotgun sequence.
ACCESSION   DASUGJ010000816 DASUGJ010000000
VERSION     DASUGJ010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801368"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..955
                     /locus_tag="VFV93_18375"
     CDS             62..955
                     /locus_tag="VFV93_18375"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5167381.1"
                     /translation="MMIKKILCAIDRSPSSLQAFGYAIALARWQSARLNLLEVVEEAP
                     PPGVTRAPKSDGVPNETRTALDRDLRRALTARRASDVKVEISMRKGNAVQEILAQAKT
                     SRADLVVIGSHGRGGVQRLVLGSVAEKVLRLATCPVLTVRRGVRLVRRSRSPFETILC
                     PTDFSAAANKAVAYAKRLAQEADAKLILMAAVEWPFGDEVASGAVAELQKSIASNAND
                     ALMRLLPRPASNGPRAQAIVAVGKASAAIVKVARARSADLIVMGVSGRGALDVAILGS
                     TTHHVIRDGACPVLTVPTGRR"
     gene            complement(1025..1408)
                     /locus_tag="VFV93_18380"
     CDS             complement(1025..1408)
                     /locus_tag="VFV93_18380"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HEX5167382.1"
                     /translation="MDKDEREDLNRNGIDDEIEPPMHDVEAGTKQLADRFQNNQGTDP
                     TLSGGDIDARWEDAESGGDETVGGSTATPGQNDLDKMGKAVGVTYADTEELRIGEKRG
                     SRDKNRWELDPASSEDYVERTNNQE"
     gene            complement(1500..1802)
                     /locus_tag="VFV93_18385"
     CDS             complement(1500..1802)
                     /locus_tag="VFV93_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167383.1"
                     /translation="MGTTIAIVLVSAARLGSLIGSRGQRIPLRFMGLIHCVDRNSDSV
                     GQSVRDFPHAAGYAHRQTTHRRRVMPLIKSWRAVDLEQLRDEAVDLRSLHAGPSAN"
BASE COUNT          366 a          622 c          634 g          404 t
ORIGIN      
        1 agttcggttt cacgcctgag cacgtcatcg agaccgcgaa aagagttctt ggaaagaacc
       61 gatgatgatc aagaagatcc tgtgcgcgat cgatcgttcg ccgagctccc ttcaggcctt
      121 cggctatgcg attgcactcg caagatggca gagcgcccga ctgaacttgc tggaagtcgt
      181 cgaggaggcg ccgcccccgg gcgtcacccg agcgccgaag agcgacggcg tgccaaatga
      241 aacccggaca gccctcgacc gcgatctcag gcgagctctg acggcccggc gcgcgtctga
      301 cgtgaaggtc gagatctcta tgcggaaggg caacgccgtt caggaaattc tcgcgcaggc
      361 gaaaacgagt cgggcggacc ttgtcgtgat cggaagccac ggccgcggag gcgtacagcg
      421 cctggtgctg ggatcggtcg ccgagaaggt tcttcggttg gccacatgcc cggtgctgac
      481 tgttcgtcgt ggtgtgaggc tggttcgccg aagccggtca cctttcgaaa cgatcctgtg
      541 tcccacggat ttctctgcgg ctgcgaacaa ggccgtggcg tacgcaaagc gtttagcgca
      601 agaagcagat gccaaactga tcttgatggc tgctgtcgag tggccgttcg gtgacgaggt
      661 ggcgtccgga gcggtcgcag aacttcagaa gagcatcgca tcgaatgcga acgacgccct
      721 catgcgtctg ttgccgcgtc cggcttctaa cggaccacgc gctcaggcga tcgtggccgt
      781 cggcaaagcg agcgctgcga ttgtcaaggt cgcccgtgcg cgatcagcgg acctcattgt
      841 gatgggcgtc tcgggacgag gcgcattgga tgtggccatc cttggatcga ccacgcatca
      901 cgtcattcgc gacggggcgt gcccggtgtt gaccgtgccg acagggaggc ggtgacactc
      961 gcgtgcgcga ctcgaatgtg gcgctcacga tcccgatccg gcgccgccca ctccgcgcct
     1021 cgggttactc ctgattgttc gtgcgctcta cgtagtcttc agaagaggcc ggatcgagct
     1081 cccatcggtt cttgtcacgg cttcctcgct tttctccaat cctcaactcc tcggtatcgg
     1141 cgtaggtaac gcccaccgcc tttcccatct tgtcgaggtc gttctggccg ggagtggccg
     1201 tgctgcctcc aacagtctcg tctcctcccg attcagcatc ctcccatcgt gcatcgatat
     1261 cgccgcctga gagcgtcgga tctgtcccct gattgttctg gaagcgatcg gcgagctgct
     1321 tcgtgccggc ctccacgtcg tgcattggcg gctcgatctc atcgtcgatc ccgttccggt
     1381 tgagatcctc tcgttcgtct ttgtccattt tgaacactcc tctcgaaggc agacgagaga
     1441 gcaacggcca tgccgcggcg ctgccagcga ggccgtcgat cgacgcgtgt cccgagggtt
     1501 cagttggctg acggacccgc gtgcagtgag cgcaggtcaa cggcttcgtc ccgaagttgc
     1561 tcgagatcga ccgcgcgcca ggacttgatg agcggcatga cgcggcgacg atgtgtcgtc
     1621 tgtcgatgcg cgtaaccagc ggcatggggg aagtcacgta cactttggcc aacgctgtcc
     1681 gaattccggt cgacgcagtg gatcaatccc atgaaccgca gcgggattcg ctgccctcga
     1741 gagccgatca ggcttccaag ccgcgccgcc gagacgagaa cgatagcgat cgtcgtgccc
     1801 acgatgacga ccgcgagtaa caacatcatg gtgtcggccc acctttcgac tcgggaggac
     1861 aacgtcagga ctgtgatgat ggcgccagac cctcgctgag gctgcatcga tcgccacgtc
     1921 gcacgtcgtg gctctcgcct tctcgtcgcc tccaggtcga gcgcgtgctg taccagccga
     1981 agctgcagtc aacaaggagg tgtagatggt caactaaaag cggtag
//
LOCUS       DASUGJ010000817         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1663569, whole genome shotgun sequence.
ACCESSION   DASUGJ010000817 DASUGJ010000000
VERSION     DASUGJ010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663569"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFV93_18390"
     CDS             <1..208
                     /locus_tag="VFV93_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167384.1"
                     /translation="HAFDPTHGRRADLSYITIATGRDYADVAPTSGSFIAPYGGVLSA
                     SKHAEITSLEWRVDDEGDENRGAA"
     gene            complement(205..>2277)
                     /locus_tag="VFV93_18395"
     CDS             complement(205..>2277)
                     /locus_tag="VFV93_18395"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5167385.1"
                     /translation="YAVLTPIYEYLALILASWGIIRSALRSGRSTSLALLAGAGLVLL
                     GVILGDGNTHGAPFAMLAMLTLVLALRRDLFRSFVAFWTAAVFFGLSVAGEKMPWLEV
                     HLAVPLALLAALTVNDLIAGFRSSHAQTDESGAAALSAQRKAPTAAIAGVAIVAGLVA
                     AALLIWPGDTSARMPFVLTLLGIAIVAIIAGGVRSRAFGAVTLAAALIALLLPLTVRD
                     GIRATFINSDTPHDLLIYTQTSPRLDQIAGKIDELARVSGEGDNLPIMIDSSDAFTWP
                     WAWYLRDYKNVTYIDLTTYPRQDLPNLRRPSVLVTLANNAPVASNWPGAFGPPERFPH
                     RWWFPEEAYRSTTGPRFWEWLRQPKMWSAWLDFYLHRTVPFPIGSIDAVAFFPPDFVS
                     QSDLDQQAAAGPRREGERLIVGGLGARAGMFNGPTSVAIDPADNLLVADTSNSRIQKL
                     SPTGEFLAASPPNVGLLEPWGVAADSLGNVFVADTWNHRIVQLDSDLRFVRAWGGPSA
                     SGQADDPSPLELYGPRGIAVDSDGNIWVTDTGHNRVLKFSPDGEPLGQFGGPGSEPGQ
                     FSEPVGIATTSGGDFLVVDTWNGRVQRFDSDFQPAGSYQVAGWEGGNTENKPFVGELA
                     DGSVVVSVPDGGRIESIGADGRGRTSWDNFGEDGKVIRPVGIAVDGRGRIWVADSAGS
                     TLIRLPAP"
BASE COUNT          437 a          707 c          791 g          342 t
ORIGIN      
        1 gcatgcgttc gacccgaccc acggtcgtcg agccgaccta agctacatca ctatcgcgac
       61 cggtcgggac tacgccgatg tggcgcccac gagtggcagc ttcatcgcgc cgtacggcgg
      121 cgtcctcagc gcgtctaagc atgcagagat tacgtcgctg gagtggcgcg tggatgatga
      181 aggggacgag aaccggggag cagcctaggg cgccggcagt cggatcagcg tcgaaccagc
      241 cgaatcggca acccagatcc ggccacgtcc gtccacggcg attccaaccg gtcgaatgac
      301 cttgccgtcc tcgccaaagt tgtcccagga ggtccgtccg cgaccgtctg cgccgatcga
      361 ctcgatccgg ccgccgtcgg ggacagagac gaccacgctc ccatcggcaa gttcgccgac
      421 gaacggtttg ttctcggtgt tgccaccttc ccaaccggcg acctgatagc tgccggccgg
      481 ttgaaagtcg ctgtcgaacc gttgcacgcg gccgttccag gtgtcgacga cgaggaagtc
      541 accgccggat gtggtcgcga tcccgacggg ttcgctgaac tgccccggct cgcttcccgg
      601 tccgccgaac tgcccaagcg gctcaccgtc ggggctgaac ttgagcacgc ggttatgtcc
      661 agtatcggtg acccagatgt tgccgtcgct gtcgacggcg atcccgcgcg gtccatagag
      721 ttcgagcggc gaaggatcgt cggcctgacc ggaggcggac ggaccgcccc acgcgcggac
      781 gaagcgcagg tcactgtcca actgaacgat gcgatggttc caggtatcgg cgacgaagac
      841 gttgcccaag ctgtcggcgg caacacccca cggttcgagc aatccgacgt tcggcgggga
      901 ggcggcgagg aattccccgg tcggcgagag cttctgaatg cggctgttgc tcgtatcggc
      961 gacgagcagg ttgtctgccg gatcgatcgc gaccgatgtc ggtccattga acataccggc
     1021 ccgcgcgccg agtccgccga cgatcagccg ttctccctcg cgtcgcggcc cggccgctgc
     1081 ctgctggtcg aggtccgact gtgagacgaa gtccggcgga aagaaggcaa ccgcatcgat
     1141 cgaaccaatc gggaacggaa ccgtgcgatg caggtagaag tcgagccagg ccgaccacat
     1201 cttcggctga cgcagccact cccagaagcg cggtcccgtc gtcgagcgat acgcctcctc
     1261 ggggaaccac cagcggtggg ggaaccgctc cggcgggccg aaggcgcccg gccagttcga
     1321 ggcgacgggc gcgttgttgg ctaaggtcac gagcaccgac gggcgtcgga ggttgggcag
     1381 atcctggcgc gggtaggtgg tgaggtcgat ataggtcaca ttcttgtagt cgcgcaggta
     1441 ccaggcccag ggccaggtga aggcgtcgga cgagtcgatc atgatcggca ggttgtcgcc
     1501 ctcaccgctc acccgcgcga gctcgtcgat cttcccggcg atctggtcga gccgggggga
     1561 ggtctgagta tagatcagca gatcgtgagg agtgtcagag ttgatgaaag ttgcacgaat
     1621 gccgtcgcgg acggtgagcg gcaacagcag tgcgatcaga gcggcggcca gtgtgaccgc
     1681 gccgaacgca cgcgaacgaa ccccgccggc gatgatcgcc acaatcgcga tgccaaggag
     1741 ggtcaggacg aacggcatcc gggcgctggt gtcgcccggc cagatcaaga gagcagcagc
     1801 gaccaatccg gcgacgatag cgacgcccgc gatggcagct gtgggagcct tcctttgggc
     1861 tgacagagcg gcggcgccgc tttcatcggt ctgtgcatga ctgctccgga agccggcgat
     1921 gaggtcgttc acggtgagcg ctgccagcag ggccagaggg acggcaaggt gaacctccag
     1981 ccacggcatc ttctctccgg cgacagagag gccgaagaag actgcggcgg tccagaaggc
     2041 gacgaacgaa cggaacagat cgcggcgaag ggcgaggacc agagtgagca tcgcgagcat
     2101 ggcaaagggc gcgccgtgcg tattgccatc gccgaggatc acgccgagca acacgaggcc
     2161 ggccccggcc agcagcgcca gcgacgtcga tcgaccggag cggagggcgg atcgaatgat
     2221 gccccaggac gcgaggatca gggcgaggta ctcgtagatc ggcgtgagca cggcgta
//
LOCUS       DASUGJ010000818         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_968213, whole genome shotgun sequence.
ACCESSION   DASUGJ010000818 DASUGJ010000000
VERSION     DASUGJ010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968213"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1626
                     /locus_tag="VFV93_18400"
     CDS             34..1626
                     /locus_tag="VFV93_18400"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX5167386.1"
                     /translation="MSATDQHADEFPLHPGSLEGITRLRIDCTELDFSVEADPSLVGT
                     IHLVVAGSSNNAPSLIREGEELIVYQRGRFRDSGNPATLLVPVENCPPISGSHEKGEL
                     LFDQVVASIALKHGSGDIRIAEGAGDISIDTGKGDVTISGREGAINVRSGMGDMRIAR
                     ARGAMNVALGKGDVDLDTCDGEIDVKLGSGDINASDCSGGLAVKGGHGDVSITRPRAA
                     LLTINSGNGDITIRGGTLAGLDARTAKGDISCGSHLLFIPDAAATSSQATATVTIEPD
                     DPNPVSRLLRSRGIEFMAGDKGLRIASGPFEFEASDAGLRIAKGKFTFEASDQGVRII
                     NGEGEDLGELGAFQLVSANGDVSVDVPSGTPLRVEALVSGGEVRSDVPLVSVGRPGPR
                     GSTQRFVGVTEPHAGQRLNLRVRSDRGDVRIRSVAGLPAQPNPPLPPSSPNAPTSRIP
                     RTPRTPRVPVTPPIPPRPARPARPANPVSGITDAIAMPAPVSQPQPSREVRMRAILNA
                     LANGAITVSEADRRLAELERDA"
     gene            1949..3241
                     /locus_tag="VFV93_18405"
     CDS             1949..3241
                     /locus_tag="VFV93_18405"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5167387.1"
                     /translation="MVASAIDSELTCGTEPVEPATRRDRLAIPALIAAAAISSIGNNL
                     TSIAIPWFVYVTTGSATRTGLVAVAGLLPVAIGGLLSGPIVDRLGFKQASVISDIASG
                     ITVAIVPTLYLLDLLEFWHLLVLSFLGALLDSPGWTARSAMIPRLARKVAMPLERANS
                     AIQLSQFGGQTIGPAIAGTLIGFVGAASVLYFDAATFALSALLIGLLVSYPMRQTGEP
                     AGEEPRASVMDDIREGLRYIVHEPFIKALLSISIFANFLFAPMFSVILPIYVKQRFDS
                     ATALGLLAAAFGVGSVAGTIFYGVFGERLPRYPFFAGGALFLTTGIWILPLSNSLIVS
                     IAGGLIAGLALGPFNIIATVALQERVPEAMLGRVIGALSMVSLASPLGVLLAGVGIDA
                     IGIRPLMAIIASGVSLIAIVIFIHPDLRKVERLTVGDA"
BASE COUNT          518 a         1160 c         1029 g          582 t
ORIGIN      
        1 cgcgacggtg gccgggggaa gggattaacg atcatgtccg ctaccgatca gcacgcagac
       61 gagtttccac tccaccccgg atcgctggag ggcatcacgc ggctgcgtat cgactgtacc
      121 gagctcgact tctcggtcga ggccgatccg tcgctggttg gcaccatcca cctggttgtg
      181 gccggctcca gcaacaacgc tccgtcgctc attcgtgagg gtgaagaact gatcgtctac
      241 cagcgcgggc gattccgcga cagcggcaat ccggcgacgt tgcttgtacc ggttgagaat
      301 tgtccgccga tttccggctc gcacgaaaaa ggtgagctgc tgtttgacca ggtcgtcgcc
      361 tccatcgctc tcaagcacgg ctcgggcgac atccgtatcg ccgaaggggc aggcgacatc
      421 agcatcgaca ccggcaaggg cgacgtgacg atctccggcc gcgagggtgc catcaacgtc
      481 cgttccggga tgggcgacat gcggattgcc cgcgcccgtg gggctatgaa tgtggcactc
      541 ggcaagggcg atgtcgacct cgatacctgc gacggcgaga tcgacgtcaa gctcggctcc
      601 ggcgacatca acgccagcga ttgcagtgga ggcctggcag tcaagggcgg ccacggcgat
      661 gtctccatca cccgcccgcg cgcggcgctg ctgacgatca acagcgggaa cggcgacatc
      721 acgattcgcg gcggcacgct ggcgggtctc gacgcgcgga cggcaaaagg cgacatctcc
      781 tgcgggtcgc acctgctgtt cattcccgac gccgccgcga cgtcgtctca agcgacagcg
      841 accgtcacga ttgagccgga cgatcccaat cccgtttcgc gactgctccg ttcccgtggg
      901 atcgagttca tggccggcga caaggggctg cggatcgcct ccggcccgtt cgagttcgag
      961 gccagcgacg ccggacttcg catcgccaag ggcaagttca ccttcgaggc ctcggaccag
     1021 ggcgtccgca tcatcaacgg cgagggcgaa gatctcggtg agctgggcgc gttccagctc
     1081 gtcagcgcca atggcgacgt ctcggtcgac gtcccgtccg ggacgccgct gcgggtcgag
     1141 gcactcgtca gcggcggtga agtgcgctcc gatgtgccac tcgtcagcgt cggccgtccc
     1201 ggcccgcgtg gctcgaccca gcgcttcgtc ggcgtcaccg agccacacgc cggccagcgt
     1261 ctcaacctgc gcgtccgttc agaccgggga gacgtccgta tccgcagcgt cgccggcctg
     1321 cccgcccagc cgaacccgcc gttgccgcca tcctcgccga acgctccgac atcgcgaatc
     1381 ccacgcacgc cgcgcacgcc acgagtgcct gttacgccgc cgatcccgcc gcgacccgcc
     1441 cgcccggccc ggcccgccaa cccggtctcc ggtatcaccg acgcgatcgc gatgccggcg
     1501 ccggtttccc agccacaacc cagccgcgag gtccgcatgc gcgccatcct caacgccctc
     1561 gccaacggcg cgatcaccgt cagtgaagcc gaccgccgcc tcgccgaact ggagcgtgac
     1621 gcatgagcgc ggtgcgatct gagaactggg acgtcgatcg tgctctggga tgtgggatgg
     1681 aggtcatgct gtcgctcccg gccagttccg ccagactgga tgacgcagac ggacgcagcc
     1741 acaaccactg tggagccggc ttggctgtgt cgccctgttc ggaaggcagc gcgagccacc
     1801 ggctcggttg ccgagaacga ctgatacgtt gataaccggc tcgtaggtcg agcctgatcg
     1861 cagtcacatc aaccgtcgcg aaagcccggc gcaggccggg aggggggtcg tattgcgtcc
     1921 ggatggacgc ctggaaggga cagggggcat ggtggccagc gctatcgaca gtgagctcac
     1981 ctgcgggacc gaaccggtcg agccggccac acgccgcgat cgactcgcga tcccggcgtt
     2041 gatcgctgcc gccgcgatct cgtcgatcgg taacaacctg acctcgatcg cgatcccctg
     2101 gttcgtctac gtcacgaccg gcagcgcgac acggaccgga ctcgtcgccg tcgccggcct
     2161 gctgccggtc gccatcggcg gactcctcag cggcccgatc gtcgaccggc tcggcttcaa
     2221 gcaggcgagc gtcatcagcg atatcgccag cggcatcacg gttgcgatcg ttccaacgct
     2281 ctatctcctc gacctgcttg agttctggca cttgctggtg ctgtcattcc tcggcgcgct
     2341 gctcgactcg cccggctgga cggcccgctc ggcgatgatc ccgcgcctgg cacgcaaggt
     2401 cgcgatgccg ctggagcgcg ccaattccgc catccagctc tcgcagttcg gcggccagac
     2461 gatcggcccg gccatcgccg gaacgctgat cggcttcgtc ggtgccgcca gcgtcctcta
     2521 cttcgatgcc gccacgttcg cactctcggc cctgctgatc ggcctgctcg tttcataccc
     2581 aatgcgccag accggagagc cagcagggga ggaaccgcgg gcgtccgtca tggatgatat
     2641 tcgcgagggc ttgcgctaca tcgtccacga gccgttcatc aaggcgctgc tcagcatctc
     2701 gatcttcgcc aatttcctgt tcgcgccgat gttctccgtc atcctcccga tttacgtcaa
     2761 gcagcgcttt gacagcgcga ccgcactcgg cctgctggcc gctgccttcg gtgttggctc
     2821 ggtcgccgga accatcttct acggcgtctt cggcgaacgc ttgccgcgct acccgttctt
     2881 cgccggtggt gcgctcttcc tgacgaccgg tatctggatt ctgccgttgt cgaattcgtt
     2941 gatcgtcagc atcgctggcg gcctgatcgc cggcctcgcc ctcggaccct tcaacattat
     3001 cgcgaccgtc gccctccagg agcgcgtccc ggaggcgatg ctcggccgcg tcatcggcgc
     3061 gctctcgatg gtctcgctgg cctcaccgct cggcgtgctg ctggccggcg tcggcatcga
     3121 cgcaatcggc atccgtccac tcatggccat catcgcctca ggtgtctccc tgatcgcgat
     3181 cgtcatcttc atccaccccg acctgcgcaa ggtcgaaaga ctgacggtag gtgacgcgtg
     3241 acgcgtgatg cgtgacgcga gggattctcg tccgactcta tcgcgtcac
//
LOCUS       DASUGJ010000819         6965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_689599, whole genome shotgun sequence.
ACCESSION   DASUGJ010000819 DASUGJ010000000
VERSION     DASUGJ010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689599"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFV93_18410"
     CDS             complement(<1..755)
                     /locus_tag="VFV93_18410"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEX5167388.1"
                     /translation="MTLEPATVAVVCAMDSEAVHLRRRLEGPREEPLARWRRTRGTIG
                     TVPVDVIVCGIGLINAAAATSALCTLAPPAAIINYGCSGAHRDDIDPGDVVIASHVVH
                     FSSLIVLPDGARRYEGFRYFVDYEEVLADRLAPAPALLERAIDVAKRVELPEWDGVGH
                     QPQVLVGTVGSADIWTQHGESIRDLHTIHGSLCEEMEAAAIAQVAATWGVPFLAIKDI
                     SNNELRQYTDMEIEGSSILAHVVDEVGRRAALIV"
     gene            complement(752..1117)
                     /locus_tag="VFV93_18415"
     CDS             complement(752..1117)
                     /locus_tag="VFV93_18415"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEX5167389.1"
                     /translation="MDERQPAGIMRRQTGLYRRIVRVMNGGFLLAIGLMLAGIVFGLI
                     TGDHISRKTDGLRDVVPGVFRLDAQDIVELGILALLATPAAYIIVALLTFLRERDLLF
                     VMICLALLGILALSVGLSL"
     gene            complement(1117..1941)
                     /locus_tag="VFV93_18420"
     CDS             complement(1117..1941)
                     /locus_tag="VFV93_18420"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5167390.1"
                     /translation="MGIGLVALGAGTLGTMLGLGGGVILVPILTLVFGISLETAVAAS
                     AIAVVANSISGSGFYLQARMTNVRLALLLLVSMTAGALAGGLLVVSLPDSITKSLFAL
                     LLIYVAFVMARRRAPALPASSVKITLPPDPHGLAGRYQDTAVNQIVDYVPHGLRVGVP
                     VASLGGLASGMFGIGGGPITVPLLTLVMGLPVKAAASTSSFMVGLTASTSGVIYYTHG
                     TVNPRVTVAAVFGIVIGARVGTRISSRIQPARLTQVFVVVLVVLGVSMALQAAGVL"
     gene            complement(2256..2729)
                     /locus_tag="VFV93_18425"
     CDS             complement(2256..2729)
                     /locus_tag="VFV93_18425"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5167391.1"
                     /translation="MEINQAGRDADVTEFGLGTLLRMPYQVMITETLEPALAAAGFGE
                     IRSAHLPVIRALSSNPDGLRATELAMYARITKQSAGYLVDNLSAGGYVERVPDPTDQR
                     AKMVRLTPRGWTAGRTILATVRGVEADWAQRIGAARITCLRQILQELVISLNAER"
     gene            2817..3161
                     /locus_tag="VFV93_18430"
     CDS             2817..3161
                     /locus_tag="VFV93_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167392.1"
                     /translation="MANRQPAHDSFLKKDGGGQVAVAMMLDLPGVTEAQYVTAREALG
                     TALPLGNLVHVAGPTATGWRVVEVWKSSTLMASYFQSSTTLKAFQTAGIRTVQPSIFP
                     DSVIAAAACASR"
     gene            3253..3729
                     /locus_tag="VFV93_18435"
     CDS             3253..3729
                     /locus_tag="VFV93_18435"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5167393.1"
                     /translation="MTHSTVDIVVNPSDETFQLGPLVIRFLVTGDDTRESASVFEFMV
                     PAGEMLRAPAHSHDAFEETVYGLEGVLTWTVDGRPIEVAPGQALCIPRGAVHRFDNLG
                     QEDARVLVIASPALFGPAYFRDMAAAVAEAASGHPDQARMADIMLRHGLTPASPAA"
     gene            complement(3982..5358)
                     /locus_tag="VFV93_18440"
     CDS             complement(3982..5358)
                     /locus_tag="VFV93_18440"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5167394.1"
                     /translation="MVELAYEPMYVVAERIARREVSPVELVETALARVATDGERLNPF
                     ITLTADLAREMAREAEREIANGGYRGPLHGIPVVLKDLLQTRGVRTTGGSQILGDSVP
                     DEDATVVRLLREAGAIFVGKTGMPEFAVLPSSGNPFYGPVRNPWDVTRDTGGSSSGTA
                     AAIASGMAWCGPGSDTGGSIRIPAAACGIVGLKPTWGRVSLRGVLPLCAMMDHVGPMA
                     RTVRDTALLMQVLAGYDADDRYSRDAPVDDYVAQLEDGIAGLRVAWLVDDGHGPVERE
                     IVEAVAGGVETLRAAGVEVTEVTMPFLNALLWEVLPVLEQVEAAADYEEWLRDRPEQF
                     SEEFRSFAEAGLRTSATEIVRARRRMQHGLHEVERTLAGFDLLVSPTLSLFPPEAGVD
                     DIELVRLTCLWDNNGWPAISVPVGLSAGGLPIGFQIVGRPWHEARVLRAARVIEHSHA
                     LTFPPANR"
     gene            5489..6484
                     /locus_tag="VFV93_18445"
     CDS             5489..6484
                     /locus_tag="VFV93_18445"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX5167395.1"
                     /translation="MSVWTEEQFEVVHHHCHGEPEPSFESEAMQQLVWGRRWGVFNDV
                     GRLRTVLVHRPGDELRTVDPAMRHPDAPALVHPEAKWYFWGEDAPDIEQMQREHDALT
                     AALAANDVEIVTLDDPRPDDINAMFTRDMLIAVPGGAIIGRMGPLMRRGQELNVLRTL
                     ARIGMPVLATVHGSGTFEGGGFAMLDETHAVCAIGHRGNEEGARQVEQVLNTLGIELL
                     RVHCRGWSCHIDGELVMVDHKTALIAPHKLPWTFLETLKTLDIRTIDVDPSEANFATN
                     CLTLAPGRVLIAADCPRTTERLIAAGIEATPIPYSECHKNGGGLHCSTMPLVRDA"
     gene            6552..>6965
                     /locus_tag="VFV93_18450"
     CDS             6552..>6965
                     /locus_tag="VFV93_18450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5167396.1"
                     /translation="MVDSSSPEILIVGAGAIGCAIALELGRRGQRDVLVVDRGRAGGG
                     SSGKAAGGLRAQYSTEFTIRSTQLSQQMFRNAEEALGGSIGYDEAGYIILARTAEQAE
                     AFRENVALQQSLGVEVSLLSPDDMERGWPWIQTDGI"
BASE COUNT         1212 a         2406 c         2223 g         1124 t
ORIGIN      
        1 acaatcagcg cggcccggcg gccaacttca tcaacgacgt gggcgaggat gctgctgccc
       61 tcgatctcca tgtccgtgta ctggcgtagc tcgttgttgg agatgtcctt gatcgccaga
      121 aacggcacgc cccaggtcgc ggcaacctgc gcgatggcag ccgcctccat ctcctcgcag
      181 agcgagccgt ggatggtgtg caggtcgcgg atgctctcgc cgtgctgggt ccagatgtcg
      241 gccgacccga ccgtcccgac cagcacctgc ggctggtggc cgacgccatc ccactcgggt
      301 agctcaaccc tcttcgcgac gtcgatggcc ctctccagca aggccggggc cggcgctagc
      361 cggtcggcca gcacctcctc gtagtcgacg aagtagcgga acccctcgta gcgccgggca
      421 ccgtccggca ggacgatcag cgacgagaag tggacgacgt ggctggcgat gacgacatcg
      481 ccggggtcga tgtcgtcgcg gtgggcgccg gagcagccgt agttgatgat ggccgccggt
      541 ggcgccagcg tacagagtgc cgaggtggcg gctgccgcgt tgatcaggcc gatgccgcag
      601 acgatgacgt caaccggcac ggtgccgatt gtcccacgcg tccgtcgcca gcgcgccagc
      661 ggctcctcgc gtggcccctc cagcctccgg cggaggtgga ccgcttccga gtccatggca
      721 cagacgacgg ccaccgtcgc aggttcaagc gtcataacga caaccccaca ctcaacgcca
      781 ggatgccgag cagcgccagg cagatcatca cgaacagcag atcgcgctcg cgcaggaagg
      841 tcagcagagc gacgatgata taggcggccg gcgtcgccag caacgccagg atgcccagtt
      901 cgacaatatc ctgcgcatcc agccggaaga cgcccggcac gacatcgcgc aagccgtccg
      961 tcttccgcga gatgtggtcg ccggtgatga gcccgaatac gattccggcc agcatcagcc
     1021 cgatcgccag caggaagccg ccgttcatca cccgcacgat ccggcgatag aggccggtct
     1081 gccgccgcat gatcccggcc ggctgtcgct cgtccatcac aggactcctg ctgcctggag
     1141 cgccatcgag acgcccagca cgaccagcac gacgacgaac acctgcgtca gccgggccgg
     1201 ctggatgcgc gacgagatac gggtgccgac ccgtgccccg atcacgatcc cgaagacggc
     1261 cgcgaccgtc acgcgcgggt tgaccgtccc gtgcgtgtaa tagatcacgc cggaggtcga
     1321 ggccgtcagc ccgaccatga acgaactggt gctggccgcc gccttgaccg gcagtcccat
     1381 caccagcgtc agcagcggga cggtgatcgg tccgccgcca atgccgaaca tgcctgacgc
     1441 tagcccgccc agcgaggcga ccggcacccc cacccgcagc ccgtgcggga cgtagtcgac
     1501 gatctggttg acggcggtgt cctgatagcg gccggccagc ccgtgcggat cgggcggcag
     1561 cgtaatcttg acactggagg ccggcagagc cggtgcgcga cggcgggcca tcacgaaggc
     1621 gacgtagatg agtagcagcg cgaagaggct cttcgtgatc gagtccggca gcgagacgac
     1681 cagcagccca cccgccagcg cgccggccgt catcgagacg agcagcagca gcgccagccg
     1741 cacgttcgtc atgcgcgcct gcaggtagaa ccccgaaccg gagatcgagt tggcgacgac
     1801 ggcgatcgcg ctggcggcga cggccgtttc cagtgaaatg ccaaacacca gcgtcaggat
     1861 cggcacgagg ataacgccgc cgcccagccc gagcatcgtc ccgagtgtcc ccgccccaag
     1921 tgcaaccagc ccgatcccca ccagctcggc cagctctcgc cctcctcatc gtgagatgtc
     1981 cgctggatag atgatagccc tgtacggacg cggaggcgaa tcgttgcgcc atgccgattc
     2041 atcgtcgtga ccgaccgacg ggcgcgactg tgcccttgga tacacgacaa acactccggt
     2101 tgccccctac gctcacgagc acgtcagtag agccgccggc ctgcaagcgg cggcactgac
     2161 tctgtcctca taccccttga gacccctacg ggagccagcc tgggggtctc atgcgcgagg
     2221 gatctggacg tcattgttgg tcgtgtcgca tggccctacc gttcagcgtt caaactgatg
     2281 accaactcct gcaagatctg ccgcaggcac gtgattcgcg ccgcaccgat gcgctgcgcc
     2341 caatcggctt cgacccctcg taccgtcgcg aggatcgtcc tgccagccgt ccagcctcgc
     2401 ggcgtcaggc gcaccatctt cgcgcgctgg tcggtgggat ctgggacccg ctcgacatag
     2461 ccgcccgcgc tgaggttgtc gacgaggtag cccgccgact gtttcgtaat ccgagcgtac
     2521 atcgccaact ctgtggcccg tagtccgtct gggttgctgg acagcgcacg gatcaccggc
     2581 agatgggcgc tccggatttc gccgaagccg gcggccgcga gcgccggctc caatgtctca
     2641 gtgatcatca cctggtatgg cattctgagg agcgttccca acccaaactc tgtcacgtcc
     2701 gcgtcgcgtc ctgcctggtt gatctccatg ttggttcacc ccttgacaat atagtcagac
     2761 gctcttatct tatagtcaga tagtcttact ataagactat cacagttgat tgcgacgtgg
     2821 ccaacaggca gccggcgcat gactcgtttt tgaagaagga tggaggaggc caagtggccg
     2881 tggcgatgat gttggacttg cccggtgtaa cggaggcgca gtacgtgacc gcgcgggagg
     2941 cgcttggcac ggccctgcca ctggggaacc tggttcatgt ggctggacca acagccaccg
     3001 gttggcgcgt tgtcgaggtc tggaaatcct cgacgctgat ggcttcctat ttccagtctt
     3061 cgacgacgct gaaggcattc cagaccgctg gaatccggac cgtacagccg tcgattttcc
     3121 ccgattccgt gatcgctgct gccgcctgtg ccagcaggtg acgacctgac gaccctggta
     3181 gaccgagcgc aacgatggaa gacagggacc cggccatgcg gccggtgatc atccagtgca
     3241 aaggagacga ccatgaccca ttcgaccgtt gacatcgtcg tcaatccgtc ggacgaaacg
     3301 ttccaactcg gcccgctcgt gatccggttc ctcgtgaccg gagacgatac ccgtgagagt
     3361 gcctcggtgt tcgagttcat ggtgccggcc ggggagatgc ttcgggcacc cgcgcatagt
     3421 catgacgcct tcgaggagac tgtctacgga ctggagggcg tgctcacctg gaccgtcgat
     3481 gggcggccga tcgaggttgc tcccgggcaa gcgctgtgta ttcctcgcgg cgcggtccat
     3541 cgcttcgaca acctgggtca ggaggatgcg agagtgctcg tcattgcttc gccggcgctc
     3601 ttcggtcccg catatttccg cgacatggct gcggccgttg ccgaagctgc gagcggccat
     3661 ccggaccagg cgcggatggc tgacatcatg ctgcgccatg gcttgactcc agcctcgccc
     3721 gccgcttgag cggatcgcgg tcgtccctgt tcccgtatca gcgaatgtat ctttggatac
     3781 atgccgcgtc gcccggttga ggcgcagact ggaacgtgcg ctgccgtggc ggcaacccgg
     3841 cagcgtcgac acgacctccg gcctgctcgg gaagggcggg agggacctcg ggtaacctcc
     3901 tcaggtacac cccgtagcgc gacgagacct ccgggctggc tcctcggagg tctcgtcgcg
     3961 ttcttcatgt ttgcgggacg gtcagcggtt ggccggcggg aacgtcagcg catgcgagtg
     4021 ctcgatcacg cgggcagcgc gcaggacgcg cgcctcgtgc cagggccggc caacgatctg
     4081 gaatccgatc ggcagcccgc ccgcactcaa cccgaccggg acggagatcg ccggccagcc
     4141 gttgttgtcc cagaggcagg tcagtcgcac cagctcgatg tcatccacgc ctgcctcggg
     4201 cgggaagagg gacagcgttg gcgagacgag caggtcgaag ccggccagcg tccgttcgac
     4261 ctcgtgcagg ccgtgctgca tccgccgccg tgcccggacg atctccgtcg cgctcgtccg
     4321 caggcccgcc tcggcgaacg accggaactc ttcggagaac tgctccggcc ggtcgcgcag
     4381 ccactcctcg tagtcggctg ccgcctcgac ctgttccagc accggcagaa cctcccagag
     4441 caacgcgttc aggaacggca tcgtgacctc ggtcacctca acgccggccg cccgcaacgt
     4501 ctcgacgccg cctgcgacag cctcgacgat ctcccgctcg accggcccat gtccgtcgtc
     4561 gacgagccat gcgacccgca ggccggcgat gccgtcctca agctgggcga cgtagtcatc
     4621 aaccggcgca tcgcgcgagt aacgatcgtc cgcgtcgtag ccggccagca cctgcatcag
     4681 caacgcagtg tcgcgaacgg tgcgtgccat cggtccgacg tggtccatca tcgcgcagag
     4741 cggcagcacg ccacgcagcg agacccgccc ccaggttggt ttcagcccga cgataccgca
     4801 ggccgccgcc ggaatgcgga tcgatccgcc cgtatccgaa ccggggccgc accacgccat
     4861 cccggacgcg atcgccgctg ccgtgccgct gctcgatcct ccagtgtcgc gcgtcacatc
     4921 ccacgggttg cggaccgggc cgtagaacgg attcccggag cttggcagaa ccgcgaactc
     4981 gggcatcccg gtcttcccga cgaagatcgc cccggcctcg cgcagcagcc ggacgaccgt
     5041 cgcatcctcg tccggcaccg agtcgccaag gatctgcgag ccgccggtcg tccggactcc
     5101 acgcgtctgc agtaggtcct tcaggacgac cggtatcccg tgcaacggcc cgcgataccc
     5161 tccgtttgca atctcccgct ccgcctcgcg cgccatctcg cgtgccagat cggccgtcag
     5221 cgtgatgaac gggttcagtc gctcgccgtc cgtcgccacc cgcgccagcg ccgtctcaac
     5281 cagctcgacc ggcgacacct cccgccgtgc gatgcgctcc gcgaccacat acatcggctc
     5341 gtacgccaac tccaccatcg tcgcctcctg tccaatgcag tgtcgattgt cgtcgcactg
     5401 cacgcggcta gcaaccatga accgttgccc aaccacccac caccccgtac catccacgcg
     5461 tcacgcgtca caaacaagga gcacacagat gagcgtctgg accgaagaac agttcgaagt
     5521 cgtccaccac cactgtcacg gtgagccgga gccgtcattc gagtcggagg cgatgcagca
     5581 gctggtctgg ggccgacgct ggggtgtttt caatgacgta gggcgactac gaaccgtcct
     5641 cgttcaccgg cccggcgacg agctgcggac ggttgacccg gccatgcggc acccggacgc
     5701 tccggcgctc gtccaccccg aggcgaagtg gtacttctgg ggcgaggacg cgcccgacat
     5761 cgagcagatg cagcgcgagc acgacgcgct gacggccgcg ctggcagcca acgacgtcga
     5821 gatcgtcacg cttgacgacc cgcgcccgga cgacatcaac gccatgttca cccgcgacat
     5881 gctgatcgcc gtccctggcg gggcgatcat cggccggatg ggcccgctga tgcggcgcgg
     5941 tcaggagctc aacgtgctgc ggacgctggc gcggatcggg atgccggttc tcgcgaccgt
     6001 ccacggctcc ggcaccttcg agggcggcgg cttcgcgatg ctggacgaga cccacgccgt
     6061 ctgcgcgatc ggccatcgcg gcaacgagga gggcgcccgt caggtcgagc aggtcctcaa
     6121 cacgctcggc atcgagctgc tgcgcgtcca ctgccgcggc tggtcgtgcc acatcgacgg
     6181 cgaactggtg atggtcgatc acaagacagc cctgatcgcc ccgcacaagc tgccctggac
     6241 gtttctcgaa acgctcaaga cgctcgacat ccggacgatc gacgttgacc cgagcgaggc
     6301 caacttcgcc accaactgcc tcaccctcgc ccccggccgc gtcctgatcg ccgccgactg
     6361 tccccggacg accgaacgcc tcatcgccgc cggcatcgaa gccaccccga tcccctacag
     6421 cgaatgccac aagaacggcg gcggcctgca ctgctcgacg atgccgctgg tgcgtgacgc
     6481 gtagtgctca gcgcagcgcg gttatcatct tggatggaat gaaggttggt cggggttaaa
     6541 ggggaatacg attggttgac agttcatcac cggagatatt gatcgtcggc gccggcgcga
     6601 tcggttgtgc gatcgcgctg gaactggggc ggcgcgggca gcgggacgtg ctggttgttg
     6661 accgggggcg cgcgggcggc gggtcgagcg ggaaagcggc cggtgggttg cgggcgcagt
     6721 actcgaccga gttcacgatc cgctcgaccc agctgagcca gcagatgttc cgcaacgccg
     6781 aggaggcgct tggcggctcg atcggttacg acgaggccgg ttacatcatt ctggcgcgca
     6841 ccgcggagca ggctgaggcg ttccgcgaaa acgtcgcctt gcagcagtcg ctgggcgtcg
     6901 aggtcagcct gctctcgccc gacgacatgg agcggggctg gccgtggatc cagaccgacg
     6961 gcatt
//
LOCUS       DASUGJ010000820         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1106902, whole genome shotgun sequence.
ACCESSION   DASUGJ010000820 DASUGJ010000000
VERSION     DASUGJ010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106902"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VFV93_18455"
     CDS             complement(<1..403)
                     /locus_tag="VFV93_18455"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5167397.1"
                     /translation="MSATSTAISPLRVRSSAYLDFIEVWLANLPDLSLDTVIADAGSN
                     AQVALVSVDLIVGFCHSGPLSSPRVAGILPAVRELFQRADRAGITNIALTQDTHRPDA
                     EEFASYPPHCIAGTVESHTAPELANLPFADSF"
     gene            complement(407..1090)
                     /locus_tag="VFV93_18460"
     CDS             complement(407..1090)
                     /locus_tag="VFV93_18460"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167398.1"
                     /translation="MQQRPPPTDEAVEIALSVRVIPFAVGRDGLEVVHYRVPSPQSQS
                     SLPSGQLQRGESLSDCASRVAQETLSIPPDYLEQLYTFSVAGPVVEAIVAYSAILSAD
                     TRAHVRLNAGAHFQHVDALDGLSDVDCGILDYAKMRLRAKLGYSNIGFYFLPEQFTLS
                     ELQDVYESVIGRPLDKRNFRRRVLAAGIVKAVGVKRPTSHRPASLYRFAGGDPATDAL
                     TPGDTDWSL"
     gene            1201..2004
                     /gene="recO"
                     /locus_tag="VFV93_18465"
     CDS             1201..2004
                     /gene="recO"
                     /locus_tag="VFV93_18465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX5167399.1"
                     /translation="MDARDPAIDEHDDQPVERRSRLYRVRGIVLRRRDLGEADRIITI
                     FTAERGKLRIVAKGARRPSSKLAGHLEPFCATRLLVARARNLDIVSQAETIEHFSTLR
                     LNETAIATAGYLAELIDALLPEDQQQEAIYELSFASLRLLNDGHDPRLVTHVFEMGLL
                     RQLGYRPELLRCVSCGAEIAPEPNGFAYDGGVLCPNCLRVRPDACSLSVNALKLLRTI
                     DRGDIERMFALRVPSQIWAEVGDVLSGYIARVTGRESSARRVLESLRLE"
     gene            2062..2766
                     /locus_tag="VFV93_18470"
     CDS             2062..2766
                     /locus_tag="VFV93_18470"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5167400.1"
                     /translation="MSYLSELLAQDEQIKVVAHRHILFLFVHTALYILGAIALWTLAG
                     IAYAQLSTGKTILTLILLAASLIPIMIAIYRFMSWRLEQYAITSYRILQVEGIINKRT
                     FDSSLEKVNDIQMTQGMFGRLFGYGNIDIITGSDVGINHLHGIAEPFVFKRTLLEAKL
                     QLSDDDRPRRVVTGVSMASQSAAQQTTPPAAPQATATSGNEAARLMAALVDLRDSGVI
                     SAAEYEERRNQLLQQL"
     gene            complement(2795..>3523)
                     /locus_tag="VFV93_18475"
     CDS             complement(2795..>3523)
                     /locus_tag="VFV93_18475"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167401.1"
                     /translation="FSERERETIAFHEAGHAIVAHYLPQGDPVSKVTIVSRGQAGGFT
                     MFVPEEDRGLWTRQQLTDRLASMLGGFAAEEIIFGEVTTGSSNDLERASSMTLSMVSR
                     YGMGQAFGLLSTGDDGAFSFSQRTTYAAESEARELVSTARDIARRILIEHRAELDLLA
                     ERLLEVETVEAAELIEMLGPAVTAGPRQKRPTPPVSVVPVSAVRPAKQSTRRRPAERL
                     LAIPAAIITSARPARRNRMTDTAN"
BASE COUNT          632 a         1119 c         1167 g          605 t
ORIGIN      
        1 tgaacgagtc cgcgaagggc aggtttgcca gttccggcgc ggtatgtgac tccacggtgc
       61 cggcgatgca gtgcggcggg tagctggcga actcctcagc gtcaggtcga tgggtgtcct
      121 gcgtgagcgc gatattggtg atgcctgccc ggtccgcgcg ctgaaagagc tcccgaacag
      181 ccggcagtat cccggcgaca cgcgggctgg agagtggccc gctgtgacag aagccgacga
      241 tcagatcgac cgacaccaac gcgacctgtg cgttcgatcc ggcgtccgcg atgacggtat
      301 cgagcgacag gtccggcagg ttcgccagcc agacctcgat gaagtcgaga tacgcagacg
      361 accgcacgcg cagtgggctg atcgccgttg atgtggcgct cattggtcag agactccaat
      421 ccgtgtcacc gggggtcagc gcgtcggtgg cagggtcgcc acctgcgaag cggtagagcg
      481 acgccgggcg gtggctggtc ggacgcttga cgccgaccgc tttcacgatc ccggccgcca
      541 gaacgcgccg gcgaaagttg cgcttgtcga gtggccggcc aatgaccgat tcgtagacat
      601 cctgcaattc ggagagcgtg aactgttcgg gcaggaaata gaagccgatg ttgctgtagc
      661 ccagcttggc tcgcagccgc atcttggcgt agtcgaggat gccgcaatcg acgtcgctga
      721 gaccgtcgag cgcgtcgacg tgctggaagt gcgcgccggc attcaacctg acatgcgcgc
      781 gggtatctgc cgacaggatg gcggagtagg cgacgatcgc ttcaacgact gggccggcga
      841 cgctgaaggt gtacaactgt tcgagatagt caggcgggat cgagagcgtc tcctgtgcca
      901 ctctggacgc gcaatcggac agcgactctc cacgttgcag ctggccggat ggcagcgacg
      961 attgcgactg tggggacggg acacgatagt ggacgacctc aaggccgtcg cgaccaacgg
     1021 cgaacgggat gacccgtacg ctcaacgcga tctcgacggc ttcgtccgtt ggcggcggtc
     1081 gctgctgcat ctcccctgcc atcacgtcgc ccgcgcttcg ggcacactta gcggagtata
     1141 gagaaccgga ccggggcacg ccattcggca gcccggcccg ggacgacagg caaggcgagg
     1201 atggacgctc gcgatcctgc aatcgatgaa cacgacgatc agcccgtaga acgccgttca
     1261 cggctctacc gcgtgcgtgg catcgtcctg cgccggcgcg acctgggcga ggcggacagg
     1321 atcatcacga tctttacggc cgagcggggc aagctgcgca tcgtcgccaa gggcgcacgc
     1381 cggccctcca gcaaactggc cggccatctg gagccgttct gcgcgacgcg gctgctggtt
     1441 gcccgggcgc gcaacctcga catcgtctcg caggccgaga cgatcgagca tttctcgacg
     1501 ctgcgtctca acgagacggc gatcgcgact gccggctacc tggcagaact gatcgacgcg
     1561 ctcctgccgg aggaccagca gcaggaagcg atctacgagc tgtccttcgc atcgctgcgt
     1621 ctcctcaatg acggccacga tccccggctg gtgacacacg ttttcgagat gggcctgctg
     1681 cgccagctgg gttaccggcc ggagctgttg cgctgcgtca gttgtggtgc ggagattgcg
     1741 ccggaaccga atggattcgc ctacgatggc ggcgtgctct gcccgaattg cctgcgggta
     1801 cggcccgacg cctgtagcct gtccgtcaac gccttgaagc tcttgcggac gatcgatcgc
     1861 ggcgacatcg agcggatgtt cgcgctgcgc gtcccatccc agatctgggc agaggtcggt
     1921 gatgtgctgt ccggatacat cgcgagggtg acaggacgtg agtcgtccgc ccgccgtgtt
     1981 ctggagtcgc tcaggttaga atgacacaaa caccaacgat gagtcagtcc ggccccgatt
     2041 gccggtgagc ggaggaagac ggtgtcgtat ctcagtgaac tgctggcgca ggatgagcag
     2101 atcaaggtcg tcgcccaccg ccacatcctg tttctctttg ttcacaccgc gctgtacatc
     2161 ctcggtgcaa tcgcgctctg gactctggcc ggcatcgcct acgcgcaact atcgaccggc
     2221 aagacgatac tgacgctgat cctgctggcg gcgtcgctga tcccgatcat gatcgccatc
     2281 tatcggttca tgagctggcg actggagcag tacgcgatca ccagctaccg catcctccag
     2341 gtggaaggaa tcatcaacaa gcgaacgttc gactcgtcgc tggagaaggt caacgacatc
     2401 cagatgaccc aggggatgtt cgggcggctg ttcggctacg gaaacatcga catcatcacc
     2461 ggctcggacg tcggcatcaa ccatctgcac ggcatcgccg agccgttcgt gttcaagcgg
     2521 acactgctcg aagccaagct ccaactctcc gatgatgacc gcccacgccg ggttgtcacg
     2581 ggagtctcga tggcgtcgca atcggcggcc cagcagacga caccgccggc tgcgccacag
     2641 gcgaccgcaa cgtccggcaa cgaggccgcc cggctgatgg ccgcgctcgt cgatctgcgc
     2701 gactccggcg tcatcagcgc cgccgaatac gaagaacgtc ggaatcagct cttgcagcag
     2761 ctgtaagtat cgctcctgcg cggggctgtg acgatcagtt ggcggtgtcg gtcatccgat
     2821 tacggcgggc tggtctggcc gacgtgatga tcgcggccgg gatcgccagc aggcgctcgg
     2881 ctggtcggcg gcgggttgac tgcttcgccg gccgaacggc gctgaccggc acgaccgaga
     2941 ccggcggggt cgggcgcttc tggcgcgggc cggccgtgac ggctggtccg agcatctcga
     3001 tcagctcggc ggcctcgacc gtctccacct caagcaaccg ctcggccagc aggtccaatt
     3061 cggcgcgatg ctcgatcagg atacggcggg cgatgtcgcg ggcggtgctg accagttcgc
     3121 gcgcctcgga ttcggccgcg taggtcgtgc gctgcgagaa gctgaacgcg ccgtcgtcgc
     3181 cggttgagag cagcccgaac gcctggccca taccatagcg cgacaccatc gagagcgtca
     3241 tgctcgaggc ccgttcgaga tcgttggacg agccagtcgt cacctcgccg aagatgatct
     3301 cctcggcggc gaagccaccc agcatcgacg ccagccggtc ggtcagctgc tggcgcgtcc
     3361 agaggccacg gtcctcctca ggcacgaaca tcgtgaatcc gccggcctga ccgcgtgaga
     3421 cgatcgtcac cttcgagacc ggatcgccct gcggcaggta gtgggcaacg atcgcgtgac
     3481 cggcctcatg gaaggcgatc gtctcccgtt ctcgctcgct gaa
//
LOCUS       DASUGJ010000821         6619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_968481, whole genome shotgun sequence.
ACCESSION   DASUGJ010000821 DASUGJ010000000
VERSION     DASUGJ010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968481"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..533)
                     /locus_tag="VFV93_18480"
     CDS             complement(186..533)
                     /locus_tag="VFV93_18480"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5167402.1"
                     /translation="MSYKQLSPQLMASIAERFRVLAEPLRLQILSELYEREQTVTELM
                     DTLGQSHANISKHLQLLYKYRFVDRRKAGLRAYYRLANEDVFELCDIMCDRLKADATQ
                     QLEIIDGILNSTT"
     gene            769..1131
                     /locus_tag="VFV93_18485"
     CDS             769..1131
                     /locus_tag="VFV93_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX5167403.1"
                     /translation="MPFEAAIEAIKAALKVEGFGVLSEIDVKATLNEKIGVDFEPYVI
                     LGACNPQLAHRALQAEHEIGLLLPCNVIVHAHADQETAVSIMDPQAALGIVANDAVGP
                     IATEARERLERVIAALAD"
     gene            1349..1693
                     /locus_tag="VFV93_18490"
     CDS             1349..1693
                     /locus_tag="VFV93_18490"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5167404.1"
                     /translation="MVLRKWLGGGIQIPQRSVTAVADSMRQRAVQVIDVREHDEWRAG
                     HIEGAIHIPLGALASRMATLDRDIPVITVCRSGRRSMAAATALKRAGFSDVANMEGGM
                     LAWARLGFYVNR"
     gene            1983..3059
                     /gene="msrA"
                     /locus_tag="VFV93_18495"
     CDS             1983..3059
                     /gene="msrA"
                     /locus_tag="VFV93_18495"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5167405.1"
                     /translation="MFFNRSKSRMISADEALPGRGQPAYEIPERHTVLGTPIQPPFPE
                     GMEVAYFGLGCFWGAERLFWKLDGVYTTASGYQGGFTPNPTYQEVCSGRTGHAEAVMV
                     VFDPAKISYEELLRVFFEAHDPTQGMRQGNDVGSQYRSAIYTTSEAQSHIAAATKAMY
                     NSELQKARHGEITTEIAPAGPFYYAEEYHQQYLDKVPNGYCGLAGTGVTCPIPAAVSG
                     GSGTETDTGLKQIPTSEDEWKRILSREEYAVLRQAGTERPFTGEYVDTEDDGIYRCRA
                     CGNILFESDTKYHSGCGWPSFTETVSPDAVELLEDRSHGMVRTEVRCARCHSHLGHVF
                     NDGPIDRGGQRYCMNSVSLTLEEQ"
     gene            complement(3266..4879)
                     /locus_tag="VFV93_18500"
     CDS             complement(3266..4879)
                     /locus_tag="VFV93_18500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX5167406.1"
                     /translation="MSTTSQMEPGADLTHRQIMVVLGGVMIGMLLAALDQSIVGVALP
                     RIVSDLGGLNHLAWVVTAYLLTSTATTPLWGKISDLYGRRKIFQATIVIFLIGSALCG
                     LAQNMPQLIAFRAIQGIGGGGLMAIAFAIIGDVIPARERGRYQGYFGAVWGLSSVAGP
                     LIGGYLTDAISWRWIFYINVPIGAAALVVTSVALRMPVIKRNHKIDYLGATMIVTAVT
                     ALLLYLNWAVEEYGWGGTTSLALVGVAFVFTIAFIFVELRAEEPILPMRLFRNQIFTV
                     GNAFLFLIGFAIFGGAIYLPLYLQTVLGMSPTESGLGMLPMVVGLFTMSVASGLLITK
                     TGKYKIYPIVGSVILIVALYLLHTIEYDTPYWTVAIYAYLFGTGLGLGMATIVTPVQN
                     AVDMRDMGVATSATTFGRSLGGAIGAALFGAILTSRLGVYLVDTLAGSGTAPSEIDTN
                     NIQAMRALPEPLRTDVLIAYTHAITDIFLMAIPVIVVALIVVCFLKEIPLRTHNYLSS
                     RHEPSDANAGEEAEAESEAEMNVPAFTGH"
     gene            complement(4983..5732)
                     /locus_tag="VFV93_18505"
     CDS             complement(4983..5732)
                     /locus_tag="VFV93_18505"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5167407.1"
                     /translation="MTGTFSSFRRLLAVVDDVRTDELVLPWAATLATDLKLPLTLLYV
                     SAPGEAYVAPSALAILRSDRRLTDLPVETRHEAGRLDNVLPTIAAEEPGTLVLLPQPV
                     DASQTQRITQWSTYDMLVSMTAPFALIPAGNVAPNHIRSVVVGNDGSELAEQVLAAVR
                     SLGVDVVEVLVYEPEDTDTYGTDPVITPNLIRANGLASRVLLRSARARDAGLIILGSH
                     GLGRASMPLAGRTTEWLLANSDRPVVVVPRS"
     gene            complement(5729..5938)
                     /locus_tag="VFV93_18510"
     CDS             complement(5729..5938)
                     /locus_tag="VFV93_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167408.1"
                     /translation="MSRLVSWLTEQAYRRAAIVIIAVVYNLADGMRNRVARRFGRGVA
                     ATPATALRPVAGSSTRMIQESGEGT"
     gene            complement(5935..6468)
                     /locus_tag="VFV93_18515"
     CDS             complement(5935..6468)
                     /locus_tag="VFV93_18515"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5167409.1"
                     /translation="MKSVESPGQDTDRRQRRRQQLEELSETLTLRMRALWNPTEWSEV
                     ELTMPQFRALDLLVKEPLRMSEIAAALGTSLQATTSLIDRLVDKGLVERGHDVADRRV
                     VICHLTPAGQTEMHRFFRMGQARLDLLIDLLTDEELDTVISAFVTLAEAALRRQEAAA
                     ATGSTPDRPNEKEKHES"
BASE COUNT         1253 a         1988 c         2177 g         1201 t
ORIGIN      
        1 gaacggagct tggcggcgat gctgcgcgtc gcggatgcgg ccggtcgttc aacccgcgaa
       61 tagagcgctt gcctccgagc aagaggccat aagtgcgccc tgtcgaccga aaagagtgac
      121 cttcctgctg actgttctga gcgacacgat cgcggatcag cgaggaagct gttccggtgg
      181 cggtcctatg tcgtggaatt gaggataccg tcaatgatct ccagttgctg tgtcgcgtcg
      241 gccttcagcc tgtcacacat gatgtcacag agctcgaaca cgtcctcatt cgcaagccgg
      301 tagtacgctc gtagccccgc tttgcggcga tcgacgaatc tgtatttgta gagaagctgc
      361 agatgcttcg agatgttggc gtgactttgc ccgagtgtgt ccatcagttc ggtgaccgtc
      421 tgctcgcgtt catatagttc gctcaggatc tgcaatctga gtggctcggc cagcacgcgg
      481 aatcgctcgg cgatggacgc cataagttgc ggcgatagtt gcttgtagga catcaaccct
      541 ccagtgtcgc ccaagtccat tccggaccct gaaccactac cgtcgcccgg tgatgccatc
      601 ggcttccttg acgacgtgca gagtatacaa ctatactacc aaatagttgt agatccaatg
      661 ccgttgatcg atgcgaacgg cgtattgaac gaaccgagca atacagagga gtgacagatg
      721 gcgagcgaga tcacgcggac ggcgtatggc ttcggaacga cggtcgcgtt gccgttcgag
      781 gcggcgatcg aagcgatcaa ggcggcactc aaggtggaag gatttggtgt gctaagcgaa
      841 atcgacgtca aggcgaccct caacgaaaag atcggcgtcg actttgaacc ctacgtcatc
      901 ctcggcgcct gcaacccgca gctcgcgcat cgtgcgttac aggccgaaca cgagattggc
      961 ctgttgctgc cgtgcaacgt gatcgttcac gcccatgctg atcaggagac ggccgtctca
     1021 atcatggatc ctcaggcggc gctgggcatc gtcgcgaacg acgccgttgg cccgattgcc
     1081 actgaggccc gcgagcgcct ggagcgagtg atcgcggcat tggctgacta gatcgcttcc
     1141 ggcgattgcg ttcgatggga tacgtagatg gcagaagcga tcatcgtcgt cgggatcatc
     1201 gttggctggc tcatgctcca gttggtgatc ctgcctcgtc tgggtgtctc cacctgaatg
     1261 gttccggtcc cagtgcatcg gtcggatgca ggtgaagaga cggcattgaa cagaagtgaa
     1321 tcggatacgg aaagggatag acgagtcaat ggtgctcagg aagtggctgg gcggagggat
     1381 tcagatcccg cagcggagcg tcacggcggt tgctgacagc atgcgtcagc gcgctgtgca
     1441 ggtcattgac gttcgtgaac acgacgagtg gcgcgcgggc catatcgagg gtgccatcca
     1501 cattccgctc ggcgcgctcg catcccggat ggcgacactg gaccgcgaca ttcctgtgat
     1561 cactgtctgc cgcagcgggc ggcgaagcat ggccgcggca acggcgctga aacgtgctgg
     1621 attcagtgat gtcgccaaca tggagggcgg catgctcgcg tgggcacgtc tgggatttta
     1681 cgtcaaccgc tgatcagcct gctcgcatca atatcctgat cgctgacatc tgcagtacct
     1741 gctcgtggtg atgggtatct gggcgcttgg ggagtgttcg caatatcgct gtaatgttga
     1801 cactgtacta tagtgttgtg gcttcggcgt ggggagcgta acccgtgcat ggcgcggaac
     1861 ttgccgcctt gggaaacgtg cggagccacc cgcgtgacga tatagacggt gttgatcggg
     1921 atacaaccgc gccgcgacga gaacccggca cctgacaccc gtcacccacg aaagggatcc
     1981 agatgttctt caatcgatcc aagtcgagaa tgatttcggc cgatgaggcg ctgccgggtc
     2041 gtggccagcc tgcctacgag attccggagc gccatacggt cctcgggacg ccgatccagc
     2101 cgccattccc ggaggggatg gaagtggcct acttcggtct cggctgcttc tggggcgccg
     2161 agcggctctt ctggaagctc gacggtgtct acacgacggc ctccggctac cagggcggct
     2221 tcacgccgaa cccgacctat caggaggtct gctccggccg gaccggccat gccgaggcgg
     2281 tgatggtcgt cttcgatccg gcgaagatct cctacgagga gttgctgcgg gtcttcttcg
     2341 aggcgcatga cccgactcag gggatgcgcc agggcaacga tgtcggtagc cagtatcgct
     2401 cggcgatcta cacgaccagc gaggcgcagt cccacatcgc cgccgccacc aaggcgatgt
     2461 acaacagcga gttgcagaag gcgcgccacg gcgagatcac gaccgagatc gcgccggccg
     2521 gaccgttcta ctacgccgag gagtatcacc agcagtacct cgacaaggtg ccgaacggct
     2581 actgcggcct ggccggcacg ggcgtgacct gcccgatccc ggcggctgtt agcggcggtt
     2641 ccgggacgga aaccgacact ggcctcaagc agatcccaac cagcgaggac gagtggaagc
     2701 gcattctcag ccgtgaggag tacgccgtcc tgcgccaggc cgggaccgag cgccccttca
     2761 ccggcgagta cgtcgacacc gaggatgacg gcatctaccg ctgccgggca tgcggcaaca
     2821 tcctcttcga gagcgacacc aagtaccact ccggctgtgg ctggccgagc ttcaccgaga
     2881 cggtctctcc cgacgcggtc gagctgctgg aagaccgctc gcacggcatg gtccggaccg
     2941 aggtgcgctg cgcccgctgc cactctcacc tcggccacgt cttcaacgat ggcccgatcg
     3001 accgcggcgg ccagcgctac tgcatgaaca gcgtctcgct gacgcttgag gagcagtagg
     3061 tctccagttc gcgggaattg cgattagacg acggggccgg gcaatttgcc cggtcccgtt
     3121 tgttcgtgat tcggccatca aggacaattc ccaatccggt aggggcgatt tcgcaaaatc
     3181 gcccttcttg atgcccgcga tgccaggaag ggcgatttcg caaaatcgcc cctaccggat
     3241 tggggattgg attgtgtgct gcgttctaat gcccggtgaa ggctgggacg ttcatctccg
     3301 cttctgattc ggcctctgct tcctcgcccg cgttggcgtc gctgggttcg tggcggctgg
     3361 agaggtagtt gtgcgtgcgt agcgggatct ccttcaggaa gcagacgacg atcagggcga
     3421 cgacgatgac cgggatcgcc atcaggaaga tgtcggtgat cgcgtgcgtg taggcgatca
     3481 ggacgtcggt ccgtagcggc tccggaaggg cgcgcatcgc ctggatgttg ttggtgtcga
     3541 tctcggacgg agctgtcccc gagccggcca gtgtatcgac caggtagacg ccgagccgtg
     3601 aggtgaggat cgcgccgaag agtgccgcgc cgatcgcgcc gccgagcgag cgaccgaagg
     3661 tcgtcgcgct ggttgccacg cccatgtcgc gcatgtcgac cgcgttctgg accggcgtga
     3721 cgatcgtcgc catcccgagg ccgaggccgg tgccgaacag gtaggcgtag atggcaaccg
     3781 tccagtatgg cgtgtcgtac tcgatcgtgt gcagcagata gagcgcgacg atcaggatga
     3841 ccgagccgac gatcgggtag atcttgtact tgccggtctt ggtgatgagc agaccggagg
     3901 cgaccgacat cgtgaagagg ccgacgacca tcggcagcat accgaggccg gattcggtcg
     3961 gcgacatgcc cagcacggtc tggaggtaga gcggcaggta gatcgcgcca ccgaagatcg
     4021 cgaagccgat caggaacagg aaggcgttgc cgactgtgaa gatctggttg cggaacagcc
     4081 gcatcggcag gatcggttcc tcggcgcgca gttcgacgaa gatgaaggcg atcgtgaaga
     4141 cgaacgcaac gccgaccagc gccagcgagg tcgttccgcc ccagccgtac tcctcgacgg
     4201 cccagttgag atacagcagc agcgccgtca cggccgtgac gatcatcgtc gcgccgaggt
     4261 agtcgatctt gtgattgcgc ttgatgaccg gcatccgcag cgcgaccgag gtgaccacca
     4321 gcgcggcagc gccgatcggg acgttgatgt agaagatcca gcgccagctg atggcgtcgg
     4381 tcaggtagcc gccgataaga gggccggcga cgctggagag cccccagaca gccccgaagt
     4441 agccctgata gcggccgcgc tcgcgggccg ggatgacgtc gccgatgatc gcgaaggcga
     4501 tcgccatcag gccgccgccg ccgatcccct ggatggcgcg gaaggcgatc agctgcggca
     4561 tgttctgggc cagcccgcag agggccgagc cgatcaggaa gatgacaatc gttgcctgga
     4621 agatcttgcg gcggccgtag aggtcggaga tcttgcccca gagaggtgtc gtcgccgtcg
     4681 aggtcagcag gtaggcggtc acgacccagg cgaggtggtt gaggccgccg aggtcgctga
     4741 cgatgcgagg cagggcgacg ccgacgatgc tctggtcgag ggcggccagc agcatgccga
     4801 tcatgacgcc accgaggacg accatgatct gacggtgggt caggtcagcg ccgggttcca
     4861 tctggcttgt cgtggacaat cgcagttcca atccatgacg gcggctcgct ggccgccgtc
     4921 agcgcgtatc aatttccttg ccccgggacg cttcccaacg ggcgaacgtc aatcgttctg
     4981 gttcaactcc tcggaacaac gacgaccggg cggtcgctgt tggccagcaa ccactccgtt
     5041 gtccggcccg cgagcggcat gctggcgcga ccgagaccgt gcgagccaag gatgatcaac
     5101 ccggcgtcgc gggcacgggc cgagcgcagc aggacgcggc tggccagacc gttggcacgg
     5161 atcaggttcg gggtgatgac tggatcggtc ccataggtgt cggtgtcctc tggctcgtag
     5221 accagaacct cgaccacatc gacgccgagc gagcgaacgg cggccagcac ctgctcggcc
     5281 aactccgagc cgtcgttgcc gacgacaacg ctgcgaatat ggttcggcgc gacgttgccg
     5341 gccggaatca gcgcgaacgg cgccgtcatc gagacgagca tgtcataggt cgaccactgg
     5401 gtgatgcgtt gtgtctgact ggcgtcaacc ggctgtggca gcaggacgag cgtgcctggt
     5461 tcttccgcgg cgatcgttgg cagaacatta tccaaccggc cggcctcgtg acgcgtctcg
     5521 accggcaaat ccgtcaggcg cctgtcgctg cgcaggatcg ccagtgccga aggggcgaca
     5581 taggcttcgc ccggcgcact gacgtaaagc agcgtcagtg gcagcttcag atcagttgcc
     5641 agcgttgctg cccagggcag gacaagctcg tcggtccgaa cgtcgtcgac aacggcgagc
     5701 agccggcgga aggagctgaa tgttcctgtc acgtaccttc tcctgattct tgaatcatgc
     5761 gagtagacga accggctacc ggcctgaggg ccgtcgctgg cgtggcggcc actccacgcc
     5821 caaagcggcg ggcaacgcgg ttgcgcatcc cgtcggccag gttgtagacg accgcgatga
     5881 tgacgatcgc ggcccgtcga taggcctgtt cggttaacca ggaaacgagg cgactcaaga
     5941 ttcgtgtttc tctttctcat tcgggcgatc tggggtcgat ccggtcgcgg ctgccgcctc
     6001 ttgccggcgc aaggcggcct cggcaagcgt cacgaaggca ctgatgaccg tgtccagctc
     6061 ctcatcggtc agcaggtcga tcagcagatc gagccgggcc tgccccatcc ggaagaagcg
     6121 gtgcatctcg gtctgaccgg ccggcgtcag gtgacagatg acgacgcggc ggtcggctac
     6181 gtcgtgcccc cgctcgacga gccccttgtc caccagccgg tcgatcaggc tggtggttgc
     6241 ctggaggctg gtgccgaggg cggcggcgat ctcactcatc cgcaacggct ccttgaccag
     6301 cagatcgagc gcacggaact gcggcatcgt caactcgacc tcggaccact cggtcgggtt
     6361 ccacaacgcg cgcatgcgca acgtcagcgt ctcggacagc tcctccaact gctgtctgcg
     6421 ccgctggcgt cgatcggtat cctgaccggg actctcaaca ctcttcacgc ttgacgacac
     6481 tcccaggttg acatatccaa caaccggaat agttccaaag gatggaacta taacacgcga
     6541 tgggagaatc aaccaggggt cgtgacgcgt gacgcgtgac gcgtgacgcg gagtaatgtg
     6601 ccgtcgtctc tcgcgtcac
//
LOCUS       DASUGJ010000822         4750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_132649, whole genome shotgun sequence.
ACCESSION   DASUGJ010000822 DASUGJ010000000
VERSION     DASUGJ010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132649"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1851)
                     /locus_tag="VFV93_18520"
     CDS             complement(256..1851)
                     /locus_tag="VFV93_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167410.1"
                     /translation="MRRWAAAITAAVFVVSSIPYLYGLGRQSATARFSGIVFDVVDTA
                     QYFAWMRAFSHSPLIANPLTPEPGAERFFNLQWWLLGLLAYDTPLGPNLVYQGLRIVA
                     LAGFALALWGFCRRVAPNQTLLSFSLVMLSSGFGWALIVAKQWTGELRHPLDVQVGEA
                     NTFFSAMAFPHLLVAAALMLAIYNAFLDGGITPRTRPMVLAALLTLALGFSHGYDLIP
                     TVLIPAATAAILTLRERRLSSFCWPAIVIGAAGVAPGLYALSLTRLDPDWEGVLSQYG
                     NAGVYTPAPHHLIVLLGLPLLLALPQLRPACWRATSTAQLFVRVWAVLGFGLLYIPTD
                     YQIKMLTGYQVPLCILAAQTIAGWRLPETIGRWQLPARRLALPVALIAVVLLTNVYLT
                     AWRVVDLRRDNYPYYLSSDDVRALESLDDVAGRRDIVMSSPNLGLFVPVYSDARPFVA
                     HWAQTLRYFERRDQARWFFAATTSDAERETFLRDSGIDYVISGPAEAADSAVSAPPRL
                     PLDAIGGQSTVLYRVRMSSGSSR"
     gene            complement(1848..3380)
                     /locus_tag="VFV93_18525"
     CDS             complement(1848..3380)
                     /locus_tag="VFV93_18525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5167411.1"
                     /translation="MQTSAARPATSTRSIPLRIRTGVFLSPYVLLFITLLVGYSYTPP
                     RWQDWNQNSRFDLTVAIVDRGTFRIDDYVANTGDYAIVDGHAYSDKAPGLSLLAVPVY
                     GLTRLAQPLGLRAVSERLAESGALSRTLTPGGEDLSSRRVDQMLALYLATIVCVAIPA
                     AVMGVLLAKMVERTCGCRTAAMLSALIIGLASPVFTYAQAFYGHVPAAACLVAALACI
                     VLREDATLSDRRLLAIGLLLGMGVLIEYPAALAGLPIAFWALVLARRRAVIWGTIGAI
                     PPLVALGICNWIAFGTPRPVGYEHSALWQQEHDTGYLSITYPHWDAIWGISFSPFRGL
                     FFFAPVLLLALAGVWFALRDSRQRTLAIVALASFAAMFLFVGSSAMWWGGFAVGPRYL
                     VPAVPMLAMPLGALIARINAAAPLPRLAGLAGTGLLMSVSAALVLATTVARQNYPPDT
                     IRRTLTGYVWPALEDGDIARNVAMAISLDGVVSLVPLLVVLALGVALIGHGLLRQQAA
                     TA"
     gene            complement(3467..4387)
                     /locus_tag="VFV93_18530"
     CDS             complement(3467..4387)
                     /locus_tag="VFV93_18530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5167412.1"
                     /translation="MAELSVAIVSYNTRDLLRRCLDSLLAATAGLQVEAIVVDNGSTD
                     GSQAMLAGYSPAVTLIENGANLGFAAANNVALAHATAPATLLLNSDAFVTTADVEQAL
                     ALLRDHPQIGMVGVRLLNIDGTVQAEAGTFPTFWEDVKTSVGIDQLGRRRRQASAVPG
                     AVDWVQGACMFVRTEAVKNVGGLDERFFMYSEEVDWCRRFWLAGWEVWYLPDANVVHI
                     GGASSRSDTRRRLALYTSRLGFRRRASGPGSSLLLWLLMLAGLAARVVIRPLAQLAFR
                     RRVGNQTAAADTALIAGLLRVDPLARWASS"
     gene            complement(4380..>4750)
                     /locus_tag="VFV93_18535"
     CDS             complement(4380..>4750)
                     /locus_tag="VFV93_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167413.1"
                     /translation="LYYVTFLPDKAMSTHSNYVDVLAQTGVFGLLSLLALFVALFALN
                     RRVLRVTRHAEDRATAAAIAGGIPAVAVSLWLGDWLIPFVYNQTLAGFDHAVYSWLMF
                     AVLCGLATQLRLDREVARDG"
BASE COUNT          984 a         1607 c         1516 g          643 t
ORIGIN      
        1 cgtgacagcg ccatgttcgt ggaaacgaca ccgacgataa caccaataat gatgatccag
       61 taccagcgac ggatgaactc gacgtagacg ttggtcatca ccggcgtgcg ctaccttcct
      121 gcatatcgac atcgcgcgtc aagtgtcgtc ccaagcaccc gcaacagcgc ttgcaacacg
      181 ctgacaaatt cgtgacgaga atgtcaggct aaatcgcctt gaggaggcac aggacaaccg
      241 agccggccag gcggctcatc ggctactgcc agatgacatc cgaacccggt agaggaccgt
      301 cgattggcca ccgatcgcgt caagcggcag gcgcggtggc gctgaaacgg cgctatcggc
      361 cgcctcagcc ggaccagaga taacgtagtc gatgccgctg tcgcgcagga acgtctcgcg
      421 ctcggcgtcc gacgtcgtgg ccgcgaagaa ccaacgcgcc tggtcacggc gttcgaaata
      481 tcgcagcgtc tgagcccagt gggcgacaaa cgggcgcgcg tcggagtaga ccggcacgaa
      541 aaggccgagg ttgggtgatg acatgacgat gtcacgccgc cccgcgacgt cgtcgagcga
      601 ttcgagcgcc cggacatcgt cgctggacag gtagtaggga taattgtctc gtcgaagatc
      661 gaccacgcgc caggcagtga gatagacatt cgtcagcagc acgacggcaa tcagcgcgac
      721 cggcagcgcc agccgcctgg ccggcaactg ccaccgacca atcgtctccg gcagccgcca
      781 gccggctatc gtctgcgcgg cgaggatgca gagcggcacc tgatagccgg tcagcatctt
      841 gatctggtaa tcggtcggga tgtagagcaa cccgaacccc agcacggccc agacgcgcac
      901 gaacagctgc gctgtcgagg tggcccgcca gcaggcaggc cggagctgcg gcagcgctag
      961 cagcagcggc aggcccagta gcacgatcag gtggtgcggg gccggcgtgt aaacaccagc
     1021 gttgccgtac tggctgagga cgccctccca gtccgggtcg agccgagtca acgagagcgc
     1081 gtagagccca ggtgcaacgc cagccgcgcc gatgacgatc gctggccagc agaagctgga
     1141 caaacgtcgt tcgcgcaatg tcagaatcgc cgccgtcgcg gcggggatca ggacggtcgg
     1201 gatcaggtcg tacccgtgcg aaaaacccaa cgccagcgtc agaagcgccg ccagcaccat
     1261 cggccgcgtc cggggcgtta tcccgccatc cagaaacgcg ttgtagatcg ccagcatcag
     1321 cgccgccgcg accagcaggt gagggaaggc catcgccgag aagaacgtgt tggcctcgcc
     1381 gacctgaaca tcgagcggat gccgcaattc gccggtccac tgcttggcaa cgattagcgc
     1441 ccagccgaac cccgacgaga gcatgacgag gctgaacgac aagagcgtct ggttcggcgc
     1501 gacccgccgg cagaaccccc agagggccag cgcaaaaccg gccagcgcga cgatcctcaa
     1561 cccctggtag accaggttcg gccccagcgg cgtgtcgtag gccaacagac ccagcaacca
     1621 ccactggagg ttgaagaagc gctcggcgcc cggctccggc gtcagcgggt tcgcaatcag
     1681 cggcgaatgc gagaaggctc gcatccaggc gaaatactgg gccgtatcaa ccacatcgaa
     1741 cacaatcccg ctgaaacgcg ccgtcgccga ctggcgaccc agcccgtaca ggtacgggat
     1801 cgagctgacg acgaagacgg cggcggtgat cgcggcagcc cagcgtctca tgctgtcgcc
     1861 gcctgctgcc ggagaaggcc gtggccaatc agcgcgacac caagcgccag cacaaccagc
     1921 aaggggacga ggctcacaac gccgtccagc gagatcgcca tcgcgacatt gcgcgcgatg
     1981 tcgccgtcct caagcgctgg ccagacgtag ccggtcagcg tcctgcggat cgtatcgggc
     2041 gggtagttct ggcgcgcgac cgtcgttgcc agcaccagtg ccgcagagac actcatcagc
     2101 aacccggtcc ccgccagccc ggccagccgg ggcagcggcg cggcggcgtt gatccgggcg
     2161 atcagcgcgc cgagcggcat cgccagcatc gggacggccg ggacgaggta gcgcggcccg
     2221 accgcgaacc caccccacca catcgccgac gagccgacga acaggaacat cgctgcaaac
     2281 gaggccagcg cgacaatcgc cagcgtccgc tgccggctat cgcgcaacgc gaaccataca
     2341 cccgccagcg ccagcaacag caccggcgcg aagaagaaca gcccccggaa cggcgaaaac
     2401 gagatgcccc agatcgcgtc ccagtgtggg taggtgatgc tcaggtagcc ggtgtcgtgc
     2461 tcctgctgcc agagcgcaga gtgctcgtag ccgaccggcc ggggcgtccc gaacgcgatc
     2521 cagttgcaga tccccagtgc aaccagcggc gggatagcgc caatcgttcc ccagatgacg
     2581 gcccgccggc gggccagcac cagcgcccag aacgcgatcg gaagaccagc cagcgccgcc
     2641 ggatactcga tcaggacacc catcccgagc agcagcccga tcgcaagcaa acggcgatcg
     2701 ctgagcgtcg cgtcctcgcg caggacgatg caggccagcg cggcaaccag gcaggcagcc
     2761 gccggaacat ggccgtagaa cgcctgggca taggtgaaga ctgggctggc aagaccgatg
     2821 atcagcgccg acagcatggc cgccgttcgg cagccgcagg tgcgctcgac catcttcgcc
     2881 agcaggacgc ccatgaccgc agcaggaatc gcgacgcaga caatcgtcgc caggtagagc
     2941 gccagcatct ggtcaacacg ccggctggac agatcctcgc cacccggcgt cagcgtccgt
     3001 gagagcgcac cactctcggc cagtcgctcc gagacggccc gcaggccaag cggctgggcc
     3061 agccgcgtga gtccgtagac gggcacagcc agcagcgaga gccccggggc cttgtcgctg
     3121 taggcgtggc catcgacgat ggcgtaatcg ccggtgttcg cgacgtagtc gtcgattcgg
     3181 aacgttcccc gatcgacgat tgccaccgtc aagtcaaagc gcgaattctg gttccagtcc
     3241 tgccagcgcg gcggcgtgta gctgtaaccc accaacaacg tgatgaagag caacacatat
     3301 gggctgagga acacgccagt tcggattctg agcggtatcg atcgtgtcga tgtcgcgggc
     3361 cgtgccgccg atgtctgcat cacaatcctc ctgcctgctc aggatgcaag cagcggctgg
     3421 cgaagcggcg aagcctgctt cgcaaaaggg taacagtctg ttcagatcag gaactcgccc
     3481 agcgagccag cggatcgacc cgcagcaacc cggcgatcag tgccgtgtcg gctgcggcgg
     3541 tctggttccc cacccgacgc cggaaggcca gttgcgccag cggcctgatg acaacccgcg
     3601 ccgccaatcc ggccagcatc agcagccaga gcaacaggct ggaacccggc ccgctggcgc
     3661 gacgacgaaa tcccagccgg ctcgtgtaga gagcgagccg ccggcgcgtg tcgctccggc
     3721 tcgatgcgcc cccgatgtgg acgacattgg cgtccggcag gtaccagacc tcccagccgg
     3781 ccagccagaa gcggcggcac cagtccactt cctcgctgta catgaagaag cgctcatcca
     3841 gcccgccgac gtttttgacc gcctcggtgc ggacgaacat gcaggcaccc tggacccagt
     3901 ccaccgcacc gggcacggca ctcgcttgtc ggcgacgccg cccgagctgg tcgatgccga
     3961 ccgaggtctt cacatcctcc caaaaggtcg ggaacgtccc ggcctcggcc tgaacggtgc
     4021 cgtcgatgtt gaggagccga acgccgacca tcccgatctg cggatggtca cgcagtagcg
     4081 ccaacgcctg ctcgacatct gctgtcgtta cgaacgcatc gctgttgagc agcagcgtgg
     4141 ccggcgctgt ggcgtgcgcc agcgccacgt tgttcgcggc cgcaaagccc aggttagcgc
     4201 cattctcgat cagcgttacg gccgggctat agccggccag cattgcctgc gagccgtcag
     4261 tcgagccgtt gtcaacgacg atcgcctcga cctgaagtcc ggcagtcgcg gccaacaggc
     4321 tgtcgaggca ccgccgcagc aggtcgcgcg tgttgtacga gacgatcgcg acgctcagtt
     4381 cagccatccc gcgctacttc ccggtccagt cgcagctgtg tcgccagccc acagaggacg
     4441 gcgaacatca gccagctgta aacggcgtgg tcgaatcccg ccagcgtctg gttgtaaacg
     4501 aacggaatca gccagtcacc gagccagagc gagacggcga cagccgggat tccgccagcg
     4561 atggcagctg ccgtcgcccg gtcctcggca tgccgcgtga cgcgcaggac ccgccggttg
     4621 agtgcaaaga gcgcgacgaa cagcgccagc aggctcagca ggccaaacac tcctgtctgc
     4681 gccagcacat cgacatagtt actgtgggtc gacatcgcct tgtcgggcag gaaggtcaca
     4741 tagtagagtg
//
LOCUS       DASUGJ010000823         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1107055, whole genome shotgun sequence.
ACCESSION   DASUGJ010000823 DASUGJ010000000
VERSION     DASUGJ010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107055"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFV93_18540"
     CDS             complement(<1..359)
                     /locus_tag="VFV93_18540"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5167414.1"
                     /translation="MSDEPVQLVGQVPAGLVQRFVPVGTGITLNVVERPGAGTPVICL
                     HGVWDWWRYWLPLVPEGSGSFAGRPLLMLDLRGHGDSSKPESGYSWVDYAADIVAFIT
                     ERGYERVMLAGHSLGSLT"
     gene            complement(375..1145)
                     /locus_tag="VFV93_18545"
     CDS             complement(375..1145)
                     /locus_tag="VFV93_18545"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5167415.1"
                     /translation="MADSSPNEVNVYTDPELYDIENTIVDELPILLPLAQETGGPILD
                     LACGTGRTTLPMAEAGFAITGVDISAPMLEHARRKARERGLKIEFYQQDCSRLALPLT
                     ARMATMSGHAFQEFVSNEAQDGLLRSVSRHLEPNGVFVFDTRFPSFANLHKPEGEQPW
                     TTVTDPDGRTIVATVIWSYDPVAQVQDYTMIERISDGTGLIDEKRSYGRLRYTWPQEM
                     ARLLRAQGFDLETIYGEWDGSPLSADSYEMIVVARKWR"
     gene            1318..>2001
                     /gene="pdxS"
                     /locus_tag="VFV93_18550"
     CDS             1318..>2001
                     /gene="pdxS"
                     /locus_tag="VFV93_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX5167416.1"
                     /translation="MQQSTWRTKVGLAQMLKGGVIMDVVTPEQAKIAEDAGACAVMAL
                     ERVPADIRRDGGVARMSDPALIRAIKETVSIPVMAKARIGHFVEAQVIEACDIDYIDE
                     SEVLTPADEMFHIDKHAFQIPFVCGARDLGEALRRLGEGAAMIRTKGEAGTGNVVEAV
                     RHMRTMSAQMRRATSMTRDELPAFAKEIGAPLELLREVADLGRLPVVNFAAGGIATPA
                     DAALMMQLGC"
BASE COUNT          350 a          691 c          637 g          323 t
ORIGIN      
        1 gtcagcgagc caagcgagtg accagccagc atcacccgct cgtagcccct ttcggtaatg
       61 aaggcgacga tatctgcagc gtagtcgacc cagctgtagc cggattccgg cttgctggaa
      121 tcgccatgcc cgcgcaggtc gagcatcagc agcggccggc ccgcgaatga ccccgaacct
      181 tccggcacga gcggcagcca gtagcgccac cagtcccaga cgccgtgcag gcagatgacc
      241 ggcgtccccg cgccaggccg ctcgacgacg ttcagcgtga tcccggtccc aaccggcacg
      301 aagcgctgca ccagcccggc cggtacctgc ccaacgagct gcactggttc gtcactcatc
      361 gcggatcact cccctcaacg ccacttccgg gcaacgacga tcatctcata cgaatctgcc
      421 gaaagtggcg agccgtccca ctcgccatag atcgtctcca gatcgaagcc ctgcgcccgg
      481 agcagccgcg ccatctcctg cggccaggtg tagcgcaggc ggccatacga gcgcttctcg
      541 tcgatcagtc ctgtcccgtc gctgatgcgc tcgatcatcg tgtagtcctg gacctgggcg
      601 accgggtcgt agctccagat gaccgtcgca acgatcgttc tgccgtccgg gtcggtgacg
      661 gtcgtccagg gctgctcgcc ctccggcttg tgcaggttgg caaacgaggg aaagcgcgtg
      721 tcgaagacga agacaccgtt cggctcaaga tggcggctga ccgagcgcag cagtccgtcc
      781 tgcgcctcgt tgctcacgaa ctcctgaaag gcgtggccgc tcatcgtcgc catccgagcc
      841 gtcagcggca gcgccagccg gctgcagtcc tgctggtaga actcgatctt caacccccgc
      901 tcgcgtgcct tccggcgagc atgctccagc atcggcgcgc tgatgtcgac gccggtgatc
      961 gcgaagccgg cctcggccat cggcagcgtc gtccggcccg tgccgcaggc caggtcgagg
     1021 atcggcccgc ccgtctcctg tgccagcggc agcaggatcg gcaactcatc gacgatcgtg
     1081 ttctcgatgt cgtagagctc cgggtccgtg tagacattca cctcgttggg agaactgtcc
     1141 gccaagcgcg acatcctttc cattccgcga cagtgtaacg cgaccgggcc tgacggttct
     1201 ccgtcctcga acgatcgtgt tggatgatgc caattggctg ttttgtgcca tccaagatgg
     1261 tggcatgctc gaagcaccga acatatcagg catgagcgag gaatggagac catccaaatg
     1321 cagcagtcca cttggcgaac gaaggtcggg cttgcccaga tgctcaaggg cggcgtcatc
     1381 atggacgtcg tcacgccgga gcaggccaag atcgccgagg acgccggcgc ctgtgcagtc
     1441 atggcgctgg agcgggtgcc ggccgatatc cgccgcgacg gcggcgtcgc gcggatgagc
     1501 gacccagccc tgatccgcgc gatcaaggag accgtctcga tcccggtcat ggccaaggcg
     1561 cgcatcggcc acttcgtcga ggcgcaggtg atcgaagcct gcgacatcga ctacatcgac
     1621 gagagcgaag tcctgacgcc ggccgacgag atgttccaca tcgacaagca cgccttccag
     1681 attccgttcg tctgcggcgc gcgcgacctc ggcgaggcgc tgcgccggct cggcgagggc
     1741 gccgcgatga tccgcaccaa gggcgaggca ggcaccggca acgtcgtcga ggccgtccgc
     1801 cacatgcgga cgatgtcagc ccagatgcgc cgtgccacgt cgatgacccg cgacgagctg
     1861 ccggccttcg ccaaggagat cggcgcgccg ctggagctgc tgcgcgaggt ggccgacctc
     1921 ggccggctgc cggtcgtcaa cttcgcggcc ggcgggatcg cgaccccggc cgacgcggcc
     1981 ctgatgatgc agctcggctg c
//
LOCUS       DASUGJ010000824         3716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_968650, whole genome shotgun sequence.
ACCESSION   DASUGJ010000824 DASUGJ010000000
VERSION     DASUGJ010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968650"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFV93_18555"
     CDS             <1..990
                     /locus_tag="VFV93_18555"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5167417.1"
                     /translation="LPSVVIPDLGLMIDHAAYAGVAALAIVMIGFSQSTGDARAFATK
                     HRYRVDINQESLAQGAANIGAGLFQGIPVSTSLSASSLNDNSGAKSQLASLVTGGVVI
                     LTMLAFAPLFSELPTPVLGAVIIDAVIMGMIDVPELQRMFHVKQSDFWISIATIVGVL
                     LAGVLAGIVIGVILSVGWLVRTVTSPAMPVLGREPGTHVFREVELYPDDEQLPGLLVL
                     GLDGDLFFATAEAVGDRLRKLALASQPPPTTIVLDCRSVFQIDSQGSAQVREMLDFAR
                     VNGIALHLARVKPQVLDVLMRDALVDEIGAANIHESLNDAVQTHLTLSRLPAA"
     gene            complement(1038..1556)
                     /locus_tag="VFV93_18560"
     CDS             complement(1038..1556)
                     /locus_tag="VFV93_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5167418.1"
                     /translation="MATVLICGNVFDGVADTLRDRTEILIQDSWIVAMAESVIRPSLV
                     EVIDLTRLTIMPRPIMRGLLPGREESRELYLLVQSGIAAVRVLQAATSSAAVLMGRPD
                     LGVLAPGKRANMIGVFGDPFEDISVVGKPGFVMVDGRIERRPSRRSVPGVNGQPERRS
                     LDRLVLKMQDLG"
     gene            2024..2797
                     /locus_tag="VFV93_18565"
     CDS             2024..2797
                     /locus_tag="VFV93_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5167419.1"
                     /translation="MAVRIERDGPVAVVTMSRPEAFNAFNTEQLRALRDAVAEVSADR
                     AIRAVVLTGEGRRAFASGADIKEMSTMRPSEGLAFGQLGQSVTAAIEAAPQPWIAAIN
                     GYALGGGCEMALACDIRLASENAAIGQPEVTLGILPGWGATQRLSRLVGAGLASELIL
                     TGRRLTAGEAREMGLVNAVYTLDELLPKATDMARAIAANAPLAVSAAKRAILLSRDVD
                     LTTGLAYEAQTFGLLFDSEDQKAGMTAFLEKRTAEFEGN"
     gene            2897..3652
                     /locus_tag="VFV93_18570"
     CDS             2897..3652
                     /locus_tag="VFV93_18570"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX5167420.1"
                     /translation="MNILVAIKQVPDPNAIRFDLSGAFAASTPRVVNEYDGYALEEAI
                     KTKEAGKADEVVVLCVGTANARDAIQRGLAMGADRGILVQASDSDLTSMTVAAIIASE
                     ARNGGYDLIFAGQESSDGGTGNTGPQLAALLDIPLVSNVVRLEIDGGTLTLQREVEDG
                     RHIVEVTPPAVICALTGLNEPRSPSLKGIMAARKKPIEEKSIGDYDTPDSQVEWGRLY
                     SEERTVQATIIQGDDPEAAAKQVVELLREKKLI"
BASE COUNT          670 a         1196 c         1241 g          609 t
ORIGIN      
        1 cttccgtccg tcgtcatccc cgatctgggc ctgatgatcg atcacgcagc ctatgccggg
       61 gtggccgcgt tggcgatcgt catgatcggg ttctcgcaga gcacgggcga cgcgcgcgcc
      121 ttcgccacca aacatcgcta ccgcgtcgat atcaaccagg agtcgctggc gcagggcgcg
      181 gccaacatcg gagccgggct gttccagggg attcccgtct cgaccagcct ctcggctagc
      241 tcgctgaacg acaactctgg cgcgaagagc cagctcgcgt cgctggtcac gggcggggtt
      301 gtcattctga cgatgctggc cttcgcgccg ctgttctccg aactgccgac gccggttctc
      361 ggcgcagtca tcatcgacgc ggtcatcatg gggatgatcg atgtgcccga gctgcagcgg
      421 atgttccatg tcaagcaaag cgacttctgg atctcgatcg cgacgatcgt cggtgtcctc
      481 ctcgcgggcg tgctggcggg aatcgtgatt ggcgtcatcc tgtcggtcgg ctggctggtc
      541 cggaccgtta cctcgccggc aatgccggtc ctcggccgcg agcctgggac gcacgtgttc
      601 cgggaggtcg agctctaccc cgacgacgag caacttcctg ggctgctggt gctggggctc
      661 gacggcgatc tgttcttcgc gacggccgaa gcagtaggag atcgcctccg caagctggcg
      721 ttggcgagtc agcctccccc aacgacgatc gtgctcgact gccgcagcgt cttccagatc
      781 gactcacagg ggtccgcaca ggtacgggag atgctggact tcgcacgtgt gaacgggatc
      841 gccctccacc tggcgcgagt caagccgcag gtgctggatg tcctgatgcg cgatgccctc
      901 gtcgatgaga tcggggctgc caacatccac gagagcctga acgacgccgt ccagacacac
      961 ctgacgttga gccggctacc ggccgcgtga tgcgggcgga cgttcgtgcg cgccgagagc
     1021 gcagccacta ccagccgtca accgaggtcc tgcatcttca agacgaggcg atcgagcgat
     1081 cgccgctcgg gctgaccgtt cacccccggc acagatctgc gcgacgggcg ccgctcgatg
     1141 cgaccatcca ccatcacgaa gccgggcttg ccgacgacgc tgatgtcctc gaagggatcg
     1201 ccaaagacgc cgatcatgtt ggcgcgcttg cctggcgcga gcaccccgag atcgggtcgg
     1261 cccatcaata cagccgccga gctggtcgct gcctgtaaga cgcgcacggc ggcgataccc
     1321 gactggacca gcaggtacag ctcacgcgat tcctcccggc cagggagcag cccgcgcatg
     1381 atcggccggg gcatgatcgt gagccgcgtg aggtcgatga cctcgacaag ggatggccgg
     1441 atcactgact ccgccatcgc cacgatccag ctgtcctgga tcagaatctc ggttctgtca
     1501 cgtagcgtat ctgcaacacc gtcgaagacg ttgccacaga tcagtaccgt tgccatgaga
     1561 tgaccccttc gccgctcagc cagcggcagt tctcctccgc gtgtaccctc agcctgacga
     1621 tcgcgccggg ccgtaactcg gcgcgagacg caggactgct ccctcgctcg aaggaatgac
     1681 gacggtgtga accggcccga gccggaccgc atccagtcat cggccgtcgt catctcacga
     1741 tccttcccag cgttctaaca tcgacaatac tcacttgccg ctgctgttgc tgcggtcagg
     1801 tggtggtaga tgggggtggc agtttccgaa acggatcagg atcgccccgc gaatggtgag
     1861 ccgggcggtg cgacaccggg catgcctgcc tgactcgtcc gcatcaacta tcatttccct
     1921 gacgatcgac gctgggcctg ccggaccgcg gaggaggagc aggagcggcg gcgcatctgc
     1981 gcagggtgtg ccgcgttcgt cgaggtggga aggagtggag acgatggcag tgcgaatcga
     2041 gcgcgacggg ccggtcgcag tggtgacgat gagcaggccg gaggcgttca acgccttcaa
     2101 caccgagcag ttgcgcgcgc tgcgggacgc ggtcgctgag gtttcagcgg atcgcgcgat
     2161 tcgggcggtc gtcctgaccg gcgaggggcg gcgggcgttc gcgtcgggcg cggacatcaa
     2221 ggagatgtcg acgatgcgac ccagcgaggg tctcgccttc ggccagctcg ggcagtcggt
     2281 gacggccgcg atcgaggcag cgccgcagcc gtggatcgcg gccatcaacg gctacgcact
     2341 tgggggaggc tgcgagatgg cgctggcatg cgacattcgc ctcgccagcg agaacgccgc
     2401 catcggccag ccggaggtga cgctcggcat cctgccaggc tggggagcaa cccagcggct
     2461 gtcgcggctg gtcggcgccg gactcgccag cgagctgatc ctgaccggcc ggcgcctgac
     2521 agccggcgaa gcacgcgaga tgggcctcgt caacgccgtc tacacactgg acgagctgct
     2581 gccgaaagcc accgacatgg cgcgggccat cgccgccaac gccccgctcg ccgtcagcgc
     2641 cgccaagcgc gccatcctgc tctcgcgcga cgtcgacctg acgaccggcc tggcctacga
     2701 agcacaaacc tttggactat tgtttgattc agaagaccag aaagccggca tgacggcgtt
     2761 tcttgagaag cgaacggcgg agtttgaggg gaattgaggt cgggaatcgg gattcgggaa
     2821 ccgtgggtat caagcggaca tgttccgttc agcactcccg aaccccgaat cccgattccc
     2881 gaacagggag ggaacattga acatactcgt cgctatcaaa caagtgccgg atccgaacgc
     2941 gatccggttc gacttatcgg gggcgtttgc ggccagcacg ccgcgagtcg tcaacgagta
     3001 cgacggctac gcgctggagg aggcgatcaa gaccaaggaa gccggcaaag ccgatgaggt
     3061 cgtcgtcctc tgtgttggga cagccaatgc gcgcgacgcg atccagcgcg ggctggcgat
     3121 gggggccgac cggggcatac tcgtgcaggc cagcgattcg gacctgacct cgatgacggt
     3181 cgccgcgatc atcgcaagcg aggcccgcaa cggcggctac gacctgatct tcgccggtca
     3241 ggagtcgagc gacggcggca ccggcaacac cggcccacag cttgccgcgc tgctggatat
     3301 cccgctcgtc tcgaacgtcg tccggcttga aatcgacggt ggcacgctca cgctccagcg
     3361 agaggttgag gacggccgcc atattgtcga agtaacgccg ccggccgtca tctgcgcgct
     3421 gacgggcctg aatgagccgc gctcgccgtc gctgaagggg atcatggccg cccgcaagaa
     3481 gccgatcgag gagaagagca tcggcgacta cgacacgccc gactcccagg tcgaatgggg
     3541 ccgcctctac agcgaggagc gcaccgtcca ggccaccatc atccagggcg acgacccgga
     3601 ggccgccgcg aagcaggtgg tcgagctgtt gcgggagaag aagttgattt gaggggtcgt
     3661 gacgcgtgac gcgtgatgcg tgacgcgaga aacgaacacc tgttacgtcg cgtcac
//
LOCUS       DASUGJ010000825         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_133262, whole genome shotgun sequence.
ACCESSION   DASUGJ010000825 DASUGJ010000000
VERSION     DASUGJ010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133262"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFV93_18575"
     CDS             <1..708
                     /locus_tag="VFV93_18575"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEX5167421.1"
                     /translation="WVVPFLNTVEEVDTRVQPHNFQQLTAATAENLQVTVTGTMNYHL
                     DAAYAGVLIQNVGTDFASKIIDPAFSDYIKQVVPAYSADSNSPVFILNRRDEIRQLTK
                     DQLNENLSRYHILVDDIYIVDIGYPEAYNASITAKQVEQQNVQREQQILEQKRIQAEQ
                     VVVTAKGQADANRETAAGQADANRSINESITDDLIQWQAIQKLNPNVNVMIVPDDGNF
                     IFSLPSAAPTTAPTPTQ"
     gene            714..1343
                     /locus_tag="VFV93_18580"
     CDS             714..1343
                     /locus_tag="VFV93_18580"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5167422.1"
                     /translation="MQRVIGPVSTNLYVLGDEASREAIAIDTATPCLEWLTATLAERG
                     WTLKLIVSTHRHWDHIGDNAAVQAATGADIAAHIADRHGLESPSGRNAPFDIPPSVPA
                     VDLAEGSRIRFGDIDLEVLHTPGHTEGSVCLLAGGERLLFTGDTLFAGSWGRIDLPGG
                     DDRLMIDSLTRLSTMADDLRVLPGHGGQTTIEREKPWLDEVARTRMLPL"
     gene            complement(1340..>2361)
                     /gene="rsgA"
                     /locus_tag="VFV93_18585"
     CDS             complement(1340..>2361)
                     /gene="rsgA"
                     /locus_tag="VFV93_18585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX5167423.1"
                     /translation="PPGTVAGRVTTEKRGGYGVAAESGDLAATVSGRLRHEAQTVEDF
                     PAVGDWVALEIAEGSQSGVIQTVLPRLTRFLRPQRGDIAQAQVVAANVDTVLLVTGLD
                     HDFNLRRLERYLALAWSSGADPVVVLNKADLAEDLGERMADVAAIAPGVPVRAVSARE
                     GTGFDGLSPLLEAGKTVALIGSSGVGKSTIVNHLLGFERQATGAVRDDDQRGRHTTTS
                     RELVTTPSGVVLLDSPGMRSVGMWEIDEGLADAFADIEALAQNCRFSDCSHGTEPGCA
                     VQAAIRDGTLPSQRLESQQKLARESAAMAMRVDPQMRAAERAKWKAIHKSVRNHMKIK
                     YGAES"
BASE COUNT          435 a          809 c          726 g          391 t
ORIGIN      
        1 tgggtcgtgc cgttcctgaa cacggttgaa gaggtcgaca cgcgagtcca gccgcacaat
       61 ttccagcagc tcactgccgc gaccgcagag aacctgcagg tgactgtaac cggcaccatg
      121 aactatcacc tcgacgctgc gtacgcgggc gtgctcatcc agaacgtcgg cacggacttc
      181 gccagcaaga tcatcgaccc ggcgttctcc gactacatca agcaggttgt tcccgcctac
      241 tcagcggaca gcaacagccc ggtcttcatc ctcaaccgac gcgatgagat ccggcagctg
      301 accaaggacc agctcaacga gaacctcagc cgctaccaca tcctcgtcga cgacatctac
      361 atcgtggata tcggctatcc agaggcatac aacgcctcca tcacggccaa acaggttgag
      421 cagcagaacg tccagcgcga gcagcagatc ctggagcaga agcgcatcca ggcggaacag
      481 gtcgttgtca cagcgaaggg tcaggcggat gccaaccgcg agacggccgc cggccaggct
      541 gatgccaacc gatcgatcaa cgagtcgata accgacgacc tgatccagtg gcaggcgatc
      601 cagaagctca atccgaacgt caacgtgatg atcgttccag atgacggcaa cttcatcttc
      661 agtctgccgt cagcggcgcc gaccaccgca ccgacgccta ctcagtgaac ctcttgcagc
      721 gggtcattgg ccccgtctcg accaatcttt acgtcctggg tgacgaggcc agtcgcgagg
      781 cgatcgcgat cgacaccgct accccgtgcc tggagtggct gaccgccacc ctggccgagc
      841 gcggttggac gctcaagctc atcgtgagca cgcaccgaca ttgggatcac atcggtgaca
      901 acgcggcagt gcaggctgct accggggccg atatagccgc gcacatcgcc gatcggcacg
      961 gcctcgagag cccgtcgggc cgaaacgcgc cgttcgacat cccaccgtcc gtgccggccg
     1021 tggatctcgc tgagggttcg cgcatcaggt tcggtgacat cgacctcgaa gtgctgcaca
     1081 cccccggtca caccgagggc tcggtgtgcc tcctcgcagg cggggagcgc ctgctgttca
     1141 ccggagacac gctgtttgcc ggcagctggg gccgcatcga cctaccgggt ggcgatgatc
     1201 gactgatgat cgattcgctc acccgtctca gcaccatggc ggacgatctg cgcgtcctgc
     1261 ccggacatgg cggccagacg accatcgagc gcgagaagcc gtggctggac gaggtcgcac
     1321 gaacgcggat gttgcctctc tagctctccg cgccgtactt gatcttcatg tggttgcgta
     1381 ccgacttgtg gattgccttc cacttggccc gctcagccgc acgcatctgc ggatcgactc
     1441 tcattgccat ggccgctgac tcgcgggcga gcttctgctg actctcgagg cgctggctcg
     1501 ggagagttcc gtcgcggatt gcggcctgga ccgcgcaacc tggctcggtg ccgtggctgc
     1561 agtctgagaa gcggcaattt tgggcaagcg cctcgatgtc tgcgaaggcg tcggccagac
     1621 cctcgtcgat ctcccacatt cccaccgagc gcataccggg gctgtcgagc agcaccacgc
     1681 cactcggcgt cgttaccagc tctcgcgacg tggtcgtgtg gcgaccacgc tggtcgtcgt
     1741 cgcggaccgc gcccgtcgcc tgccgctcga agccgagcaa gtgattcacg atggtcgact
     1801 tgcccacgcc ggacgacccg atcagagcca cggtcttacc cgcctcgagc aggggagaca
     1861 ggccgtcaaa gccggttccc tcgcgtgcgc tgaccgcacg tacgggcacg cccggcgcaa
     1921 tcgcggcgac gtccgccatg cgctcaccca ggtcctccgc gagatccgcc ttgttgagta
     1981 cgacgaccgg gtccgctccg ctggaccacg cgagggccag gtagcgctcc aggcgacgaa
     2041 ggttgaagtc atggtcgagc ccggtgacga gcaggacggt gtcgacgttg gccgcaacga
     2101 cctgtgcctg cgcgatgtcg ccacgctgcg gccgaaggaa gcgcgtcagc cgcggcagga
     2161 cggtctggat gacgcccgat tggctgccct cggcgatctc cagcgccacc cagtccccaa
     2221 ccgccgggaa gtcttcaacc gtctgtgcct cgtgccgcaa ccggccggag acggtcgctg
     2281 ccaaatcgcc ggactcggcc gcaacgccgt agccgccgcg tttctcggtg gtgacacggc
     2341 cggcaactgt gccgggagga g
//
LOCUS       DASUGJ010000826         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1664799, whole genome shotgun sequence.
ACCESSION   DASUGJ010000826 DASUGJ010000000
VERSION     DASUGJ010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664799"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFV93_18590"
     CDS             complement(<1..264)
                     /locus_tag="VFV93_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167424.1"
                     /translation="MKIEQFALERWMTTWETQVEYDIAESGIYPMSVNELLDMLPDGE
                     REATLARLLDMRLGYSEAPGSLELRSLLAATYKETGPDSILVTT"
     gene            complement(306..848)
                     /locus_tag="VFV93_18595"
     CDS             complement(306..848)
                     /locus_tag="VFV93_18595"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX5167425.1"
                     /translation="MSGLVTAVSRSATHTMTKPNEESIRLLEGLGVEGDAHLGVTVKH
                     RSRVRRDPTQPNLRQVHLIHEELIDELNGSGFDLVPGQMGENVTTRGVDLLGLPTGTR
                     LHLGETAIIEVTGLRNPCDQLDGIQPGLMKATLDRDADGNLIRKAGVMAIVILGGDVH
                     ANDPIRIELPAEPHTPLVPV"
     gene            985..>2143
                     /locus_tag="VFV93_18600"
     CDS             985..>2143
                     /locus_tag="VFV93_18600"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5167426.1"
                     /translation="MADSVREIERQILGEIWTSGDMMESLRYLCDDLGSRFGGTESEQ
                     MAAQYLQAKMREYGLQNVHLEEFPVYTWERGACELTLTAPVEREISAIAMPFCPAGDF
                     SGEVIDVGEGETADFERVGDAVRGKIVLSAAETNKPGEVSLHRTDKYRLAHEAGAIGY
                     IMANKNPGLLHITGGLYARKPNGPDDIDHDGPIPAIGISHEAAELLRRLAERGTLQVK
                     IHQESRTYRSRSANVVGDIPGQSADEIIVFGGHYDGHDVAQGAADDGAGTLVGLEVGR
                     RLAPFAGQFKRTIRIVCFGYEELGLGGSWKHADRYDNGNEKLLFAMNLDGSGRGQGGQ
                     ERITVTGDPQLRDWFATLPDELLYDVDVNNRISAHSDHFPFFLKGYPTASLS"
BASE COUNT          367 a          706 c          712 g          358 t
ORIGIN      
        1 ggttgtgacg agaatgctgt ccggaccggt ctccttgtag gtcgccgcca gcagcgagcg
       61 cagctccagc gagccgggcg cttccgagta gcccagccgc atatccagca gtcgcgccag
      121 cgtcgcctcg cgttcgccat ccggcagcat gtccagcagc tcgttgaccg acatcgggta
      181 gatgccactc tcagcgatgt cgtactcgac ctgcgtctcc caggtcgtca tccagcgctc
      241 aagcgcgaac tgttcgatct tcatggtgca tttcctatcg atgaacctgc atccgcatcc
      301 agcgatcaga ccggcacgag cggcgtgtgt ggttcggcgg gcagctcgat gcggatcggg
      361 tcgttggcgt ggacatcgcc gccgaggatg acgatcgcca tcacgccggc cttgcgaatc
      421 aagttgccgt cggcgtcgcg gtcgagcgtg gccttcatca ggccgggctg gatgccgtcg
      481 agctggtcac acggattgcg caggcccgtc acctcgatga tggctgtctc gcccagatgg
      541 agtcgggtgc cggtcggcag gccgagcaga tcgacaccac gcgtcgtcac gttctcgccc
      601 atctgaccgg ggacgagatc gaagccggac ccgttcagtt cgtcgatcag ctcctcgtgg
      661 atcagatgca cctggcgcag gttcggctga gtcggatcgc ggcgtacccg cgagcggtgc
      721 ttgacggtca cgccgaggtg cgcgtcgccc tcgaccccga gtccctccag cagccggatc
      781 gattcttcgt tcggcttcgt catcgtatgc gtcgcgctgc ggctcacggc cgtcacgagt
      841 ccagacatcg tcccgcgttc cctcccacat agccctcagg ttgtgccgtg tagtctagcg
      901 cagtctgcca ttgacacatc gcgcgtctcg tccgatcctc gcgcctcaac gaccacgcga
      961 ggaccgcagc gagaggggag cgacatggcc gattcggtac gtgagatcga gcggcagatc
     1021 ctgggcgaga tctggacctc cggcgacatg atggagagtt tgcgctatct ctgtgatgac
     1081 ctcggctcgc gcttcggcgg gaccgagagc gagcagatgg cggcgcagta cctgcaagcc
     1141 aagatgcgtg agtatgggct gcagaacgtc cacctggagg agttcccggt ctacacctgg
     1201 gagcggggcg cctgcgagct gacgctgacg gcgccggtcg agcgtgagat ctcggcgatc
     1261 gcgatgccgt tctgcccggc cggcgacttc agtggcgaag tgatcgacgt tggcgaggga
     1321 gagacggccg acttcgagcg ggtgggcgac gcggtgcgcg gcaagatcgt cctctcggcg
     1381 gccgagacga acaagcctgg cgaagtctcg ctgcaccgga ccgacaagta ccggctggcg
     1441 cacgaagccg gcgcaatcgg ctacatcatg gccaacaaga accccggcct gctccacatc
     1501 accggcgggc tctacgcgag gaagccgaac ggcccggacg acatcgacca cgacggcccg
     1561 atcccggcca tcggcatcag ccatgaagcg gccgagctgc tgcgccggct ggccgagcgg
     1621 ggcacgttgc aggtcaagat ccaccaggag agccgcacct accgctcccg ctcggcgaat
     1681 gtcgtcggtg atatcccagg ccaaagcgcg gatgagatca tcgtcttcgg tggccactac
     1741 gacgggcacg acgtcgccca gggcgcggcc gacgacgggg ccgggacgct ggtcgggctg
     1801 gaagtcgggc ggcggctggc gccgttcgcg ggccagttca agcgcacgat ccggatcgtc
     1861 tgcttcggct acgaggagct gggtctcggc gggagctgga agcacgccga ccgctacgac
     1921 aacggcaacg aaaagctgct gttcgcgatg aacctcgacg gctccggccg cggccagggc
     1981 ggccaggagc ggatcaccgt caccggcgat ccgcagttgc gcgactggtt cgcgacgctg
     2041 ccggacgagt tgctgtacga cgtcgatgtc aacaaccgca tcagcgccca ctcggaccac
     2101 ttcccgttct tcctgaaggg ctacccaacc gcatcgctct cca
//
LOCUS       DASUGJ010000827         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2080727, whole genome shotgun sequence.
ACCESSION   DASUGJ010000827 DASUGJ010000000
VERSION     DASUGJ010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080727"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1787)
                     /gene="ggt"
                     /locus_tag="VFV93_18605"
     CDS             complement(153..1787)
                     /gene="ggt"
                     /locus_tag="VFV93_18605"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5167427.1"
                     /translation="MTWPNRGLAHRPAVTGRRGAVASAHPHASMAGLRMLQQGGNAVD
                     AVVAVAAALNVVEPYMSGAGGVGYMTIAVPGRQTPIILEYIGRAPAAATLDQFSEPGS
                     KDDGIRSPLVPGALGGWLAALERFGTMDRATVFAPAIEYARDGYPVTIKNHEFMDGNR
                     ERLLRWPTSTGAYLHNGATPQPGAILKQPDLARTFEAIVEGGAEVLYKGPIGAEIARF
                     SQENGGLLTIEDLAGYTPSWQEPISTTYRGYTTFCPPLPCSGMQYLITLNIVEGFDMA
                     EMGQNSADYLHHLAEAMKLAVADRTTYAPNPSSPIEALLSKDYAATRRDLIDPARAGY
                     SGGERFTSHKLPEEILPGSFESIMRESTTHFATADADGWAVSCTQTLGGGFGSGVVHG
                     NTGLSLNNFAHWFDDDPDSPNVIAPSKQVEMCLAPSQTWRDGRLFVTVGTPGSFGIME
                     TTPQMLLNVLDHGYSIQAAIEAPRFRTITGRELIIEGRVPYAVLDDLRRRGHEVEVLD
                     PWTPLVGGGQGIMVDPDSGAFMAGADPRRDGYALAL"
     gene            2049..>2240
                     /locus_tag="VFV93_18610"
     CDS             2049..>2240
                     /locus_tag="VFV93_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167428.1"
                     /translation="MDRVRNLLQHRALLVFAMGHFTVDMFGGLMPVLYALLSDEFQLS
                     NADIGLIALAYTSASSLSQP"
BASE COUNT          337 a          750 c          736 g          417 t
ORIGIN      
        1 tgtgcgaagc ggacacgcgg aagttgcccg acccgctccg ccagcgcctc gcggctgagc
       61 gatcgactcg tcggcgttgc cgtcattcca tccccctcgt ccgttgtcta ttctcgaccg
      121 gttcgattcg gcagtggatt gtcagcgaat ggttagagcg cgagggcgta cccgtcgcgg
      181 cgcggatcgg cgccggccat gaacgcgccg ctgtcgggat cgaccatgat cccctgcccg
      241 ccgccgacca gcggcgtcca cgggtcgagc acctcgacct catgaccgcg ccggcgcaga
      301 tcgtccagca cagcataagg gacgcggccc tcaatgatca gttcgcggcc ggtgatcgtc
      361 cggaagcggg gcgcctcgat cgccgcctgg atgctgtagc cgtggtcgag gacgttaagc
      421 agcatctgtg gtgtcgtctc catgatcccg aacgaccccg gtgtgccgac cgtcacgaac
      481 agccgcccat cgcgccacgt ctgcgacggc gccagacaca tctcgacctg cttgctcggt
      541 gcgatcacgt tcgggctgtc cggatcgtcg tcgaaccagt gggcgaagtt gttgagcgac
      601 agcccggtgt tgccgtggac gacacccgag ccgaagcctc cgcccagcgt ctgcgtgcag
      661 gagacggccc agccgtcggc atcggccgtc gcgaagtggg tcgtgctctc gcgcatgatg
      721 ctctcgaaac tgcccggcag gatctcctcc ggcagcttgt gcgaggtaaa ccgctcgccg
      781 ccgctgtagc ccgcccgcgc ggggtcgatc agatcgcgcc gcgtcgccgc gtagtccttc
      841 gagagcagcg cctcgatcgg cgaggacgga ttcggtgcgt aggtcgtccg gtccgcgacc
      901 gccagcttca tcgcctcggc caggtggtgc aggtagtcgg cgctgttctg gcccatctcg
      961 gccatgtcga agccctcaac gatgttgagc gtgatcagat actgcatccc actgcacggc
     1021 agcggtgggc agaaggtcgt gtatccccga taggtcgtcg agatcggctc ctgccagctt
     1081 ggtgtgtagc cggccagatc ttcgatcgtc agtagcccgc cgttctcctg cgagaagcgg
     1141 gcgatctcgg ccccgatcgg tcccttgtag agaacctccg cgccgccctc gacgatcgct
     1201 tcgaacgtcc gcgccaggtc cggctgcttg agaatcgcgc cgggctgcgg cgtcgcgccg
     1261 ttgtggaggt aggcacccgt cgatgtcggc cagcgcagca gccgctcgcg gttgccgtcc
     1321 atgaactcgt ggttcttgat cgtgaccggg tagccgtcgc gggcgtactc gatagccggc
     1381 gcgaagacgg ttgcgcggtc catcgtcccg aagcgctcca gggcagccag ccagccgccc
     1441 agcgcgcccg gcacgagtgg cgagcggata ccgtcgtcct tgctgcccgg ctcgctgaac
     1501 tggtcgagcg ttgccgctgc cggcgcccgg ccgatgtact caaggatgat cggcgtttgc
     1561 ctgcctggca cggcgatggt catgtagccg acgccgccgg ccccggacat gtacggctcg
     1621 acgacgttca gcgccgccgc gaccgcaaca acggcgtcga cggcgttgcc gccctgctgg
     1681 agcatgcgca gcccggccat cgacgcgtgc ggatgcgccg aggcaactgc ccccctgcgt
     1741 ccggttacgg cgggacgatg cgcgagtccg cggttcggcc aggtcatagc ccctcctctt
     1801 tcgcctcgct ctgctgcatt gtggtgaatt ggattgtgcg atgtatggca gttccgcggc
     1861 ggcatcccga aaactgcgac tgacttcagg tgtgttcgca tactaacggc aaagaattga
     1921 caggtcaagg accaagcgaa cggcatcagc catcgttccg tcacgagttg cgggtgcgca
     1981 gcctcgaatc acctcgtatc atggtggatc gttgcgctgg cccggacggg aagcgatagg
     2041 gttcgtcggt ggatcgagtt cggaatctgc tccagcatcg cgccctgctg gtgttcgcga
     2101 tgggccactt caccgtcgat atgttcggcg ggctgatgcc cgttctctac gcgttgctgt
     2161 ctgacgagtt ccagctcagc aacgccgaca tcgggctgat cgcgctggcc tacacctcgg
     2221 cgtcgtcgtt gtcgcagccg
//
LOCUS       DASUGJ010000828         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_690750, whole genome shotgun sequence.
ACCESSION   DASUGJ010000828 DASUGJ010000000
VERSION     DASUGJ010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690750"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFV93_18615"
     CDS             complement(<1..382)
                     /locus_tag="VFV93_18615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX5167429.1"
                     /translation="MSSRRGGLGRGLESLIPGIGTDTERGTTSSVDIDALEPNPLQPR
                     VRWDDDQLNALAASIREHGIIQPILVTRRGTTRPFQIIAGERRWRAAQRAGLTSVPIL
                     VREATPSQVLELALVENIQRADLNA"
     gene            535..1560
                     /locus_tag="VFV93_18620"
     CDS             535..1560
                     /locus_tag="VFV93_18620"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX5167430.1"
                     /translation="MGEDIASAVSLIVSAVMLVGITAFLSLWVRRANEDELEGKRATP
                     MGLYIVFGAFGFFLFLYGLGSSYRDWREGVSPNDASYLALAVGIIAAFTLFPPLRKLL
                     AKVIPFDPNSWADTIGLFLIASVVALSSVALFVEADEQEPATYLILVVTAVTEVALAF
                     VIVGAPFYRTVKQAVKRLGLEVPTLRQVGIAMGLVFVAFAISAASSLLVRYLQPDLYD
                     RINENLGTMTSGIDRWWGAVILGLCAGIGEEILFRGAIQPKYGIIVTSIVFAVLHQQY
                     GASFVTAGVFGIGILFGLERKYVNTTACIITHVVYNVIAVLAAQAADDEREAIVHFIR
                     LAIDWLS"
     gene            complement(1587..3131)
                     /locus_tag="VFV93_18625"
     CDS             complement(1587..3131)
                     /locus_tag="VFV93_18625"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5167431.1"
                     /translation="MADHNCREAREIISAFIDGEASREESARLRQHLASCADCREVLD
                     SYRQIGGAIRGMPAVHPPYHLTESIFAQTIDAEPRRLFLITSRLGYSLAAIAAILLIF
                     VVAGYLIIGGYERGVQPTVASSEPKSSPTGADTLIWPLQDPIRIEFNKEMNHESVIAA
                     LGMQPQAEKDQLSRSWDGNTLVIGGNPPFKPNTHYEIKISTDALDKWGNPLKEPFRLA
                     FTTSSSQTMVQTPTPVILPSATPESALPTSTTGVIPPASTPTPDGSAPETATTPPGNN
                     PAPGSGNGTAVVPTNPNPPLDPTETATPPRPTPTDAPVEPTTADEPTATLTPPPTATA
                     VPPTATPTPLPPTATATIPAPTATETPDTIPVVGAIGDVYWRDQTVQDRLGKAVAPAT
                     SFVTDQLDFQRGVMMLDETRANIYVLKFGSGWDTWGVPTGEMPSAEPGPEQDIYVPGL
                     TFGLLWRSNVDLQDDLGFALSEFAISFSADVQIFENGRIISTPTTVYVIYDDTTWDWF
                     THAADS"
BASE COUNT          505 a          939 c         1080 g          660 t
ORIGIN      
        1 tcgcgttcag gtcggcgcgc tggatattct cgaccagcgc gagctcaagc acctgcgatg
       61 gtgtggcttc gcgcaccagg atcggcacgg aggtcagacc agcgcgctgg gcggcccgcc
      121 agcgacgttc accagcgatg atctggaaag ggcgcgtggt tccacggcgg gtaacgagaa
      181 tcggctggat gatgccgtgc tcgcggatcg aggcggccag cgcgttgagc tggtcatcgt
      241 cccaacggac acgaggctgg agagggttcg gctcgagcgc gtcgatgtcg acgctgctgg
      301 ttgtgccgcg ctcggtgtcg gttccgatgc cggggatcag cgattccagt ccgcgaccca
      361 gcccacctcg tctgctcgac accagccccc accactttct gtcacgcggt tccgcgtgaa
      421 gtataccgat ggccgggacc gactctgtgc aaagcgtcac aatcaggcgg aggctggcta
      481 tacttcgcgt cgggccggcc atcgccggag tgctgacgaa cggagctttc cgaattgggt
      541 gaagacatcg catcggcggt atcgctgatc gtctcggcgg tcatgctggt gggcatcacg
      601 gcgtttctca gcctctgggt gcggcgcgca aacgaggacg agcttgaagg caagcgcgcg
      661 acgccgatgg gcctctacat cgtcttcggc gcgttcggct tcttcctgtt cctgtacggc
      721 ctgggttcct cgtatcgtga ctggcgggag ggtgttagcc cgaacgatgc gtcgtacctg
      781 gcactggccg tcggcatcat cgccgcattt acgctcttcc cgccgttgcg aaaactgctc
      841 gcgaaggtca ttccgttcga cccgaattcg tgggcggaca cgatcgggct gttcctgatc
      901 gcgtcagtcg ttgcgttgtc atcggtcgcg ctcttcgtcg aggcggatga gcaggaaccg
      961 gcgacctacc tgatccttgt cgtgacagcc gtgaccgagg tcgcattggc atttgtcatc
     1021 gtcggcgcac ccttttaccg gaccgtcaag caggcggtga agcggcttgg gctggaagtt
     1081 ccgacgctgc ggcaggtcgg tatcgcgatg gggttggtct tcgtcgcatt cgccatttca
     1141 gcggcgtcat cgctactggt gcgctacctc caaccggacc tgtatgaccg gatcaacgag
     1201 aatctgggga cgatgacgtc cggcatcgat cgctggtggg gcgcggtgat cctcggactg
     1261 tgtgccggaa tcggcgagga gatcctcttt cgtggagcga tccagccgaa gtacggaatc
     1321 atcgtcacgt cgatcgtgtt cgccgtcctg catcagcagt atggtgccag cttcgtgact
     1381 gccggggtgt tcgggatcgg cattttgttc gggctggagc gcaagtacgt caacacgacg
     1441 gcctgcatca tcacccacgt cgtctacaac gtcatcgccg tcctggctgc gcaagccgca
     1501 gacgatgagc gcgaagcgat cgtccacttt atcaggctgg caatcgattg gctttcctga
     1561 tcgatgcggg tgcccggccg gattgattac gagtcggctg cgtgcgtgaa ccagtcccag
     1621 gtcgtgtcat cgtagatgac gtagaccgtc gtcggggtcg agatgatccg accgttctcg
     1681 aagatctgga cgtcggctga gaagctgatc gcaaactcgc tcaacgcgaa tcccaggtca
     1741 tcctgcaggt cgacgtttga acgccagagc agcccaaacg tcagacccgg gacgtagatg
     1801 tcctgttccg ggcccggttc cgcggatggc atctcgccgg tcgggacgcc ccacgtgtcc
     1861 cagcccgagc cgaacttgag gacgtagatg ttcgcgcgtg tctcgtccag catcatgacg
     1921 ccgcgttgga agtcgagttg atcggtgacg aacgacgttg ccggggctac tgccttcccg
     1981 agccggtcct gaacggtctg atcgcgccag tagacatcac caattgctcc gacgaccggg
     2041 atcgtgtcag gcgtttcggt tgcggtcggt gccgggatcg tcgctgtcgc tgtcggaggt
     2101 aacggagtcg gagttgctgt cggcgggaca gccgttgcgg tcggtggcgg cgttagtgtc
     2161 gccgtcggct cgtctgccgt cgttggctca actggtgcgt cggtcggcgt cggacgcgga
     2221 ggcgtcgccg tctcggtcgg atccagcggc ggattcgggt tagttggcac gacggccgtt
     2281 ccgttgccgg aacctggtgc ggggttattg ccgggcggtg tggtcgccgt ctcgggggcg
     2341 cttccgtcgg gtgtcggcgt ggaggcaggc gggataacgc cggtcgtgct ggttggcagc
     2401 gccgattcag gcgtcgcgct cggcaggatg actggtgtcg gcgtctggac cattgtctga
     2461 ctggacgatg tcgtgaatgc cagccggaat ggctccttga gcgggttgcc ccacttgtcg
     2521 agcgcgtcgg tcgagatctt gatctcgtag tgcgtgttcg gcttgaacgg cgggttgccg
     2581 ccgatgacga gcgtgttgcc gtcccacgac cgcgaaagct gatctttctc cgcctgcggc
     2641 tgcatgccga gcgcggcgat gaccgactcg tggttcatct ccttattgaa ctcaatcctg
     2701 atcggatctt gaagcggcca gatcaacgta tcggcaccgg ttggcgaact cttaggctcc
     2761 gacgatgcga cggtgggttg aacgccacgc tcgtagccgc cgatgatgag gtagccggcg
     2821 acgacgaaaa tcagcaggat cgcggcaatc gccgccagcg agtagccgag gcggctggtg
     2881 atcaggaaga gccggcgcgg ttccgcgtcg atcgtctggg cgaatattga ctcagtgagg
     2941 tggtacggcg gatggacggc cggcatgcca cggattgcgc cgccgatctg cctgtacgag
     3001 tcgagcacct cgcggcaatc ggcacaactg gccaggtgct ggcggagacg tgcgctctcc
     3061 tcgcggctcg cttcgccgtc gatgaacgcc gagatgatct cgcgtgcctc gcggcagttg
     3121 tggtcggcca tcaggaggcc tccccgtcta ccgcgacata cagcttgcgg aactcttcac
     3181 ggcc
//
LOCUS       DASUGJ010000829         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1108074, whole genome shotgun sequence.
ACCESSION   DASUGJ010000829 DASUGJ010000000
VERSION     DASUGJ010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108074"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..963)
                     /gene="proC"
                     /locus_tag="VFV93_18630"
     CDS             complement(85..963)
                     /gene="proC"
                     /locus_tag="VFV93_18630"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX5167432.1"
                     /translation="MNATTTHLDALRRKRVTFIGTGVMGEAIIAALLDGGLLEAGQIT
                     GSEPYPERREELAGRYGIRMVASNAEATDGADVVMLTIKPQSLNAIFSDLGGQLAPEQ
                     VVVSIIAGATVERLRHGLDHQRIVRSMPNTPAQIGQGVTVWMPTSAVSDEQNLVVSAL
                     LSAMGHAVRVESEDAVDMATALSGTGPAYIFLVMEALIDAGVHMGFPRRIAEELVLQT
                     MIGSVLFARESGKHPAELRNMVTSPGGTTAAALYQMEKGSLRTVISRAVWAAYQRTVE
                     LGQDNPAPVPPRERDA"
     gene            complement(1032..1907)
                     /locus_tag="VFV93_18635"
     CDS             complement(1032..1907)
                     /locus_tag="VFV93_18635"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5167433.1"
                     /translation="MKIFDTHVHFPWGEDRDVDEVIEELVERAQAAGVVHLCLLGSRW
                     GDYNARVTRAIEKYPDLFIGLYGIDLDNEGPEAVHEAAARGFRGLKVILPLKRYDDPG
                     YFPIYEAAEEHGFAALFHTGVVGGGADYRVQDPFDPELIERVRPWEQRSRGSGVSSAH
                     MEPIYLDTIAFTFPELKVIGAHLGIGYYDVAAHIARWRRNVFFDVSGGEMVRRHILER
                     KLIPSEISHFKLLYGSDNNERFEDEIRDWQTMFDMLRLDEDQRERIMYGNAARLFGMI
                     PTEVFPEPTAVGHEG"
     gene            complement(2231..2707)
                     /locus_tag="VFV93_18640"
     CDS             complement(2231..2707)
                     /locus_tag="VFV93_18640"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5167434.1"
                     /translation="MSDLNAGPELLDDPVAQELLQSRIPARLAYTWHDGTPRVVPIWF
                     HWNGEEIVVAGPPDAPKVAAIEAQPQVALTIDGDTWPYKALLLRGTARVDTTEGLPPE
                     YVAAAERYFGPEQGPQWVGMVGPMFDTTARITIRPEHVTILDFETRFPSKIASRMG"
     gene            complement(2781..>4160)
                     /locus_tag="VFV93_18645"
     CDS             complement(2781..>4160)
                     /locus_tag="VFV93_18645"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5167435.1"
                     /translation="AEYFTRMAESIYPRLYQYPIDISLYDQLHADHDNIRSALAWAIE
                     RRDAELGQRLAGSLARFWNASSDRHEALSWLERVIELGETTPAIYVRVLDAIGVMAFQ
                     VGDYERAVRVYTEAASLYRTLGNHGRLARVLDDLGSIALEQGRYDEAESLFAEALGAA
                     SAVESPDPIIAELIGPGNLALARMDLGRAGELLEGALAIRRSAGAEATVCVSYESLGY
                     LAIARGKLADARSSFEGALRIGQDLDSQELSARGHGGLGWLALVERDGQSACQQFAEA
                     LRLNHGIGLLVESVNVIEGLASAAAQLGSDREAARLFGAVERLRESAALLASPFRRCL
                     VGPFGDAARRRLSSVEWEAAWQRGRALSLDDTCQAALDIARETITARHEPPADPPARL
                     TSREMDVLRLLAVGRSNREIGAELFLSVRTVERHVANLYRKIDAHNRAEATTFAIRVN
                     LVQTESVVW"
BASE COUNT          738 a         1382 c         1310 g          730 t
ORIGIN      
        1 tcgtggaagg gcgatattgc gatatcgccc gtaccggatt gatgattgtg aattgaggaa
       61 cgggtatgac acccgcatca cacatcacgc atcccgctcc cgcggcggca ccggcgcggg
      121 gttgtcctgg ccgagctcga cggtccgctg gtaggcggcc cagacggcgc gtgagatgac
      181 ggtgcgcagg ctgcctttct ccatctggta gagcgccgcc gccgtcgtcc cgcccggcga
      241 ggtgaccatg ttgcgcagct cggccgggtg cttgcccgac tcgcgggcga acaggaccga
      301 accgatcatc gtttgcaaga ccagttcctc ggcgatccgg cgcgggaagc ccatgtgaac
      361 accggcgtcg atcagcgcct ccatgacgag gaagatgtag gccggtccgg tcccggagag
      421 cgccgtcgcc atatcgacgg cgtcctcgct ctcgacgcgg acggcgtgcc ccatcgcgct
      481 cagcagggcc gagacgacca ggttctgctc gtcgctgacg gcgctggtcg gcatccagac
      541 ggtgacaccc tggccgatct gggcgggggt gttgggcatc gagcgcacga ttcgctggtg
      601 gtcgagccca tgccgcagcc gctcgacggt cgccccggcg atgatcgaga cgacgacctg
      661 ctccggtgcg agttgcccgc ccaggtcgct gaagatcgcg ttgagcgact gcggcttgat
      721 cgtcagcatg acgacatcgg cgccatcggt cgcctcagcg ttgctggcga ccatccggat
      781 gccgtagcgc cctgccagct cttcacgccg ctccggatac ggctcgctgc cggtgatctg
      841 gccggcctcc agcaacccgc cgtccagcag cgccgcgatg atcgcctcgc ccatcacgcc
      901 ggtcccgata aaggtcacgc gcttgcggcg cagggcgtcg aggtgagtgg ttgttgcgtt
      961 catgtgttct ccgatgggga tgtgggatga ggcatggggc atgagatcag cccaacctca
     1021 tcccccatgc ctcatccctc atgcccaact gcggtcggtt ccgggaagac ctcggtcgga
     1081 atcatcccga acagccgggc cgcgttgccg tacatgatcc gttcgcgctg gtcctcgtcg
     1141 aggcgcagca tgtcgaacat cgtctgccag tcgcggatct cgtcctcgaa gcgttcgttg
     1201 ttgtcggagc cgtagagcag cttgaagtgg gagatctcgc tggggatcag cttgcgctcc
     1261 aggatgtgtc gccggaccat ctcgccgccg gagacgtcga agaagacgtt gcgccgccag
     1321 cgggcgatgt gggcggcgac gtcgtagtag ccgatcccga ggtgggcgcc gatgaccttc
     1381 agttccggga aggtgaaggc gatcgtgtcg aggtagatcg gctccatgtg ggccgaggag
     1441 acgccggatc cccgcgagcg ctgctcccag ggccggacgc gctcgatcag ctcagggtcg
     1501 aacgggtcct gaacgcggta gtcggccccg ccgccaacga cgccggtgtg aaacagggcc
     1561 gcgaagccat gctcctcggc cgcctcgtag atcgggaagt agccgggatc gtcgtagcgc
     1621 ttgagcggca ggatgacctt gaggccccgg aagccccgcg cggcggcctc gtggaccgcc
     1681 tccggcccct cgttgtcgag gtcgatgcca tacagcccga tgaacagatc cggatacttc
     1741 tcgatcgccc gcgttacccg ggcgttgtag tcgccccagc gcgaccccag caggcagaga
     1801 tggacgacgc ccgccgcctg ggcgcgctcg accagctcct cgatcacctc atcgacgtcg
     1861 cggtcttccc cccacgggaa gtggacatgc gtatcaaaga tcttcacgat tcgtggtgct
     1921 ccaaaccaac tcacgaacga cagtcacgca ggtatctcgc gtgtagtaaa gcacgataca
     1981 gcccaaagac acggaccaat cgtagtctgc cagccgcgag ccatcggcgg ctcgtgggca
     2041 gggccgccca ttcgctttgg ggaagtggtt tccatgcaac tgcaacgcgc gcgctggctg
     2101 gccgcgatgc tgctgacgat agcgctgctc ttcgtcatca acggcctgtc tggatgatga
     2161 gcacaagccc tgtgtcgcgg agggacgatc gttcctccgc gacacagggc taccgtatca
     2221 gcagatgcga tcagcccatc cgtgaggcga tcttgctggg gaagcgggtt tcgaagtcga
     2281 ggatcgtgac gtgttcaggc cggatcgtga tccgcgcggt tgtgtcgaac atcggtccga
     2341 ccatgccaac ccactgcggt ccctgctccg gcccgaagta gcgttcggca gcggcaacgt
     2401 actccggtgg gaggccctcg gtcgtgtcaa cgcgtgcggt cccgcgcaac agcaacgcct
     2461 tgtacggcca cgtatcgccg tcgatcgtca gtgccacctg cggctgggcc tcgatcgcgg
     2521 ccaccttcgg cgcgtcgggc ggccctgcaa cgacgatctc ctcaccattc cagtggaacc
     2581 agatcggcac gacacgcggc gtcccgtcgt gccaggtgta ggccagccgc gccgggatgc
     2641 gcgactgcaa cagctcctgc gcgaccggat cgtcgagaag ctctggtcca gcgttgagat
     2701 cggacatgga accctccctt gcgactagca tgcgtcgacg cagcgtttac cgtgctttcg
     2761 tcaatcgttc attaccgtaa ctaccagacc acgctttcgg tctgaaccag gttgacccgg
     2821 atcgcgaagg tcgtcgcttc agcgcggttg tgcgcgtcga tcttgcggta gaggttggcg
     2881 acatgccgct cgaccgtccg aaccgacagg aacagctccg cgccaatctc gcggttactg
     2941 cgcccgacgg ccagcaggcg aagaacatcc atttcacgcg atgtcaaacg agccggtgga
     3001 tctgcaggag gctcatgtcg agcagtgatc gtctcgcgcg cgatgtccag cgccgcctga
     3061 caagtgtcgt cgagcgacag cgcccgtcct cgctgccatg ctgcctccca ctctacggac
     3121 gagaggcgcc ggcgcgctgc atcgccgaac gggccgacga ggcaacgccg gaacggcgaa
     3181 gccagcaagg ctgccgactc ccgcagccgc tcgactgctc caaagagcct agctgcctcg
     3241 cggtcgctgc ccaactgggc ggcagcggac gccagtcctt cgatcacgtt gaccgactcg
     3301 accaacagac caatgccgtg gttcagcctg agcgcttcgg cgaactgttg acatgccgac
     3361 tgaccatcgc gttcaaccaa cgccagccag ccgaggccgc catgtcctcg agcgctgagt
     3421 tcctgggaat cgaggtcctg tccaattcgc aaagcgcctt cgaacgacga tcgagcatca
     3481 gcgagttttc cgcgagcgat ggccagatat cccagactct cgtacgacac gcacactgtc
     3541 gcctcggccc ctgccgagcg ccggatagcg agggcccctt caagcaactc acccgctcgg
     3601 ccaagatcca tgcgggccag cgcgagattg ccggggccga tcaactccgc gatgatcgga
     3661 tctggcgatt ccaccgcact ggctgctccc agcgcctcgg caaacagcga ctcggcctca
     3721 tcgtatcggc cctgctcaag cgcgatcgag ccaaggtcat ccagcactct cgcaaggcgg
     3781 ccatgatttc cgagcgtccg atagagtgag gccgcttccg tgtagacacg gactgcacgc
     3841 tcatagtcgc caacctggaa cgccatcact ccgatcgcgt ccagcacccg cacgtaaatt
     3901 gctggcgtcg tctccccgag ctcgatcaca cgttccaacc aggagagtgc ctcgtgacga
     3961 tcgctactgg cgttccagaa tcgcgcgagc gaaccggcca gtcgctgccc gagctcagca
     4021 tcacggcgct cgatcgccca ggccagcgca gaacgaatgt tgtcatgatc cgcgtgaagc
     4081 tgatcgtaga ggctgatgtc tattgggtac tgatagagtc gtggatagat ggactctgcc
     4141 atccgcgtga agtactcggc
//
LOCUS       DASUGJ010000830         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1940754, whole genome shotgun sequence.
ACCESSION   DASUGJ010000830 DASUGJ010000000
VERSION     DASUGJ010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940754"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFV93_18650"
     CDS             complement(<1..181)
                     /locus_tag="VFV93_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167436.1"
                     /translation="MKTIFLDTIAASETTVGYGTLGCGGKLGYERKRVTVGGASCPHA
                     LSTHPPARLVFPLDGQ"
     gene            complement(162..1217)
                     /locus_tag="VFV93_18655"
     CDS             complement(162..1217)
                     /locus_tag="VFV93_18655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5167437.1"
                     /translation="METPRDALLAGSAGMLPPDAKELNNRGALLHLDGNLAVARAAYE
                     QALALDPDNATALNNLGFLLAQAGELGQAIDLYERAIALDPARSVAYVNLGNALALAG
                     DADGAYEALCRAVEIDPTNGLAWENLGRLLMAAGELGASEQAFRTALALQPAQASLVT
                     ALGIVVAAQERSGEAVELLQHALALDPEDAHAWAQLGIVLFSRQDLGSAEDALSRALE
                     LAPEDTDARHHLALTLLARGRHDEAAAALVRVLEIDPANAPARVDLAVLALSDGQVDV
                     TLDQLALVLEQGPASERARFYHAVALDQAGEREQAIDELEALAGEPDGKYPVEARRYL
                     EMIGHPAEDGTDEDHLS"
     gene            complement(1240..>3334)
                     /locus_tag="VFV93_18660"
     CDS             complement(1240..>3334)
                     /locus_tag="VFV93_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167438.1"
                     /translation="WEIKDIRDTALQFDELRVLNKKQIDDKGLQGSPSRLAIFTNEAG
                     GGVRFSDGWAEGDKEKDVTFDFGKLLRVHKVSYDRSGKTGSLHGTAFKNNKIIEEASF
                     KFPLQESAAVDYGGFVPSGGVTREIGSLLKAKGLSPIEIQVAELTDRGLVARGQILPT
                     ISVLRNLKINLIIDGDDIYLSRVFSAGDFKFPGPIQVTSASLEVFAGTPGIGARGDVF
                     FEIKKLGKGKLSGKASTGSGFSVAGEFEFDTKLFDPAMVRVEYSNGKFSGSGEIGVPP
                     GKLRGIRSANIKAAFEDERITATGSVKPEIPGIEQGDLSMSYDPVGGLMIAGSLQLKK
                     DIPGISGGTVTAQVIKKPDADNYIVKASGQATSNIPGISASLSVSYDDGQFDVFGTVG
                     YEKGMLKGSVSVGATNRPIGDDGKPVPGPVDKASKIILYGGGSVTIKLAPWLQATAAI
                     KLLPNGEIEVTGSIGLPQALDLFPEKKLDRNIFKIGIDIPIVGLSVAGQRVGIFANIS
                     GGADLSAGIGPGQLRELGLSITYNPSRESETHVTGGATLHIPAHAGLRLFVRGGLGVG
                     IPLVSATAGLEIGGAIGLEGALDAGVQVDWTPTQGLKLDAFGEISVEPKLRFDITGFV
                     LVEFDYLIDTATLYEKRWQLAAFEYGSGLKFGVKFPVHYEEGKAFDISLSDVQFQTPD
                     INPKELLGDLIKQIA"
BASE COUNT          532 a         1144 c         1064 g          594 t
ORIGIN      
        1 cctggccatc gagcgggaag accagccggg ccggtgggtg ggtgctgagg gcgtgcgggc
       61 agcttgcgcc gccgaccgtg actcgcttgc gctcgtagcc gagcttgccg ccacagccga
      121 gcgtcccgta cccgaccgtc gtctccgagg cggcgatcgt gtcaagaaag atggtcttca
      181 tccgtgccat cctccgccgg gtgaccgatc atctccagat agcggcgcgc ctcgaccgga
      241 tatttgccgt ccggctcgcc ggccagcgct tcgagctcgt caatcgcctg ctcccgctca
      301 ccagcctgat cgagggcgac ggcgtgatag aagcgcgccc gctcgctggc cggtccctgc
      361 tccagcacga gcgccagctg gtcgagcgtg acatcgacct gaccgtccga cagtgccagg
      421 acagccagat cgacgcgggc cggcgcattg gcagggtcaa tctccaggac gcggacgagg
      481 gctgccgccg cctcgtcgtg cctgcctcgc gccagcaacg tcaaagcgag gtggtgacgg
      541 gcgtcggtat cctccggcgc cagctccagc gcgcggctga gcgcgtcttc ggccgaaccc
      601 agatcctgac ggctgaagag gacgatcccc agttgcgccc aggcgtgcgc atcctcgggg
      661 tcgagcgcga gcgcatgttg cagaagctca accgcctcgc cgctgcgctc ctgggcggca
      721 acaacgatgc cgagggccgt caccagcgac gcctgcgcgg gctggagcgc cagcgccgtc
      781 cgaaacgcct gctcggacgc cccaagctcc ccggcagcca tcagcagccg gccgagattc
      841 tcccaggcca gcccgtttgt cgggtcgatc tcgaccgccc ggcagagcgc ctcgtaggcg
      901 ccgtcggcgt cgccggccag cgccagcgcg ttgccgagat tcacgtaggc gacactcctg
      961 gccgggtcga gcgcgatggc ccgctcgtag aggtcgatcg cctgccccag ctcgcccgcc
     1021 tgtgccagca ggaagccgag gttgttgagc gccgtcgcgt tgtctggatc gagcgccagc
     1081 gcctgctcgt aggcggcgcg agctacggcc aaattgccgt cgaggtgcag caacgctcct
     1141 cggttgttga gctccttcgc gtccggcgga agcattcccg ctgaccccgc caacaacgcg
     1201 tcgcgcggcg tctccatcgg ttcgtatcct tcccggccgt caggcgatct gcttgatcag
     1261 gtcgcccagc agctccttgg ggttaatatc cggcgtctgg aactggacat cactgagtga
     1321 gatgtcgaac gctttacctt cctcgtagtg gaccgggaac ttaacgccga acttcaggcc
     1381 cgagccatac tcgaacgcgg ccagctgcca gcgcttctca tacagcgtcg cggtatcgat
     1441 caggtaatcg aattcgacca ggacgaagcc ggtgatgtcg aagcgcagct tcggctcgac
     1501 cgagatctcg ccgaaggcgt ccagcttgag cccctgggtc ggcgtccagt cgacctggac
     1561 tccggcatcc agcgccccct caagcccgat cgcgccgcca atctccagtc ctgccgttgc
     1621 gctgacgagc ggaatcccga cgccaaggcc accacgaacg aacagccgca gcccggcgtg
     1681 ggccgggatg tgcagcgtgg ccccaccggt gacatgcgtc tccgactcgc gggaggggtt
     1741 gtaggttatc gacagcccca gctcgcgcag ctgccccggc ccgatcccgg cgctcaggtc
     1801 ggcgccgccg ctgatgttgg cgaagatccc gacccgctgg ccggccaccg acaggccgac
     1861 gatcgggatg tcgatcccga tcttgaagat gttgcgatcg agcttcttct ccgggaagag
     1921 gtccagcgcc tgcggcaggc cgatcgagcc ggtgacctcg atctcaccgt tcggcagcag
     1981 tttgatcgcc gccgtcgcct gcaaccaggg cgcgagcttg atcgtgacgc tgccgccgcc
     2041 gtagaggatg attttgctgg ccttgtcgac cggccccgga accggcttgc cgtcgtcgcc
     2101 gatcgggcga ttggtcgcgc cgaccgagac cgaccccttc agcatgccct tctcgtagcc
     2161 gaccgtcccg aagacgtcga actgaccgtc gtcataagag acggaaagcg aggcactgat
     2221 accggggatg ttcgacgtgg cctgaccgct ggccttgacg atgtagttgt cggcgtccgg
     2281 cttcttgatg acctgggcgg taaccgtccc gccggagatg cctgggatgt ccttcttgag
     2341 ttgcagcgac ccggcgatca tcagcccgcc gaccgggtcg tagctcatgc tcaggtcgcc
     2401 ctgctcgatg ccgggaatct ccggcttgac cgagccggtc gccgtgatgc gctcgtcctc
     2461 gaacgcggcc ttgatgttgg ccgagcggat gccgcgcaac ttgccgggcg gaacgccgat
     2521 ctcgccgctg ccgctgaact tgccgttgct gtattcgaca cggaccatag ccgggtcgaa
     2581 cagcttggtg tcgaactcga actcgccggc aacgctgaac ccgctgccgg tcgacgcctt
     2641 accgctcagc ttgcccttgc cgagcttctt gatctcgaag aagacgtcac cccgtgcgcc
     2701 gatgcccggc gtcccggcga agacctccag cgaggcgctc gtgacctgga tcgggccggg
     2761 gaacttgaag tcaccggcgc tgaagacgcg gctgaggtag atgtcgtcgc cgtcgatgat
     2821 caggttgatc ttcaggttgc gcaggaccga gatggtcggc aggatctggc cgcgcgcgac
     2881 cagccctcga tcggtcagct cggcaacctg aatctcgatc gggctgagcc ccttcgcctt
     2941 cagcagcgaa ccgatctccc gcgtcacacc gccggatggc acgaaaccgc cgtagtcgac
     3001 ggcggcagat tcctgcaatg ggaacttgaa actggcttcc tcgatgatct tgttgttctt
     3061 gaacgcggtt ccgtggagtg atccggtctt tccggagcgg tcatagctga ccttgtgtac
     3121 cctcagcagc ttgccgaagt caaatgtgac gtccttctcc ttgtcgcctt cggcccagcc
     3181 gtcgctgaag cggaccccgc cgccggcctc gttcgtgaag atcgccagcc gggagggcga
     3241 gccctgcaac cccttgtcgt cgatctgctt cttgttgagg acgcgcaact cgtcgaactg
     3301 aagggcggtg tcgcggatat ccttgatctc ccag
//
LOCUS       DASUGJ010000831         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1523463, whole genome shotgun sequence.
ACCESSION   DASUGJ010000831 DASUGJ010000000
VERSION     DASUGJ010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523463"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFV93_18665"
     CDS             complement(<1..334)
                     /locus_tag="VFV93_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167439.1"
                     /translation="MTTIECVPAWVVTQREAATMTETGLHATAGPPERRGTGRGTQRF
                     LADAGAVLAASLDHDETLISITRVIVPALADWCAVTLSPANAADRTWEVAHADAALET
                     ILREQDWTL"
     gene            complement(404..1489)
                     /locus_tag="VFV93_18670"
     CDS             complement(404..1489)
                     /locus_tag="VFV93_18670"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167440.1"
                     /translation="MPATTSTSSTQSARSSTNGSSQDDSTEAAHAAGLRYVNDSEPGF
                     RRVRRGKGFSYYDPSGERLTDPATLDRIASLAIPPAWTDVWICISPRGHLQATGRDAR
                     NRKQYRYHPRWRDVRDEAKFDRMIEFGETLPAIRATVDSDLSRPGLPREKVLAIVVRL
                     LGSTHIRVGNTEYARDNHSFGLTTMRNRHVEVDGADIIFEFNGKGSKKHTVGIHDRRL
                     ARLIARLRELPGQQLFQYLDHEGTRHTISSDDVNAYLKQISGRDLTAKDFRTWAGTVL
                     AAGALADAGPSDDERQAEATVVAAIKAVAAQLGNTPTVCRACYVHPAIIEAYLDGQTI
                     ATTRSRKRLDPEEAAVLHMLKGRRDTG"
     gene            1786..2913
                     /locus_tag="VFV93_18675"
     CDS             1786..2913
                     /locus_tag="VFV93_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5167441.1"
                     /translation="MGQVIFDHVTKAFDTFVAVKDFSLDIHDGEFLVLVGPSGCGKST
                     ALRCLAGLEEITSGRIFIGERDVTYLDPKDRDIAMVFQNYALYPHMSVRDNMAFGLKL
                     RRTSKEVIAQRVNNAAEILGISQLLDRKPRALSGGQRQRVALGRAIVREPQCFLMDEP
                     LSNLDAKLRVQTRAEIIKLQKRLGTTTIYVTHDQVEAMTMGDRIAVMNAGELQQLDTP
                     QNLYNSPANRFVATFIGSPAMNVFPAMVSNGAGAPMIAAGDFKVQVPATRADRVRERA
                     GQTVLVGIRPEHIQVSEFAEDVTDGNTITMPVELVEHLGAETLLHLRGPEGDSIVARV
                     DPRTTLKAGDVAKLVVNTGQLHVFDPTTEQALTAEGAASSS"
     gene            complement(2983..3903)
                     /locus_tag="VFV93_18680"
     CDS             complement(2983..3903)
                     /locus_tag="VFV93_18680"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX5167442.1"
                     /translation="MSTVRALVVGSLVMDLSFQVPKRPEPGEVILADSFGTYRGGKGY
                     NQAVALARLGASVTMIGALGADAYGDEFIASLDREGIDTLRMVRLPQSFTSIAVPLVT
                     PDGDVGFVHAQGANAELRPDHCRDLPDADVLLLQGEIPLDTSLAVAQQASRQGTIVIL
                     NPAPVHHVTAGLIATADIIVANEVEALALLETPATSRADPLDLAQKLATDGRIAVVTL
                     GARGAVWSDGTSSGRATPPNVTAVDATAAGDSFCAALALAFVEGQRLADAVRFACAAG
                     AHAATVRGAEPGLPTRKQVDALLNEPKRSS"
     gene            complement(3900..>4769)
                     /locus_tag="VFV93_18685"
     CDS             complement(3900..>4769)
                     /locus_tag="VFV93_18685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5167443.1"
                     /translation="NTMMGDSYHIDDPAKIQGRDEIIPGTRNLGEIRLERALDALGDT
                     GGRLLLLGAGAGRYARALANARPDLDVYAGDLSRRAIAEAVSIGGTPLYLVMDAEKIP
                     FASDTFDAVIFFDLIEHVPSPVTLLAECKRVLRPGCVLHFFVPLEDQPKTLYRALRRN
                     SPIPIHRWKHKHVGHIQRFDEQRIVKLTWDAGFTVTGLSHSFHLAGQVHDIVDYWQRE
                     RAAGGAGRLPLSAVKLISRGIFVVTWRLAYLEDRAYSGAMLASGLHLTARKPPATTEL
                     VARHKSLQPAVAQ"
BASE COUNT          841 a         1460 c         1585 g          883 t
ORIGIN      
        1 tgagcgtcca gtcctgctcg cgcaagatgg tttcgagtgc ggcgtcggcg tgcgcgactt
       61 cccaggtgcg gtctgccgcg ttcgcggggc tgagcgtgac tgcgcaccag tcggcaagcg
      121 ccggaacaat cacgcgggtg atcgagatca acgtctcatc gtggtccagc gatgccgcca
      181 ggaccgcacc ggcgtcagcc aggaatcgct gcgtgccacg gcctgtgccg cgccgctcgg
      241 gagggcccgc agttgcgtga agacctgttt ctgtcatcgt cgctgcttcc cgctgcgtca
      301 caacccatgc aggcacacac tcgatcgtcg tcaacgcgat gcgatgatcg taccgcaggg
      361 tatcaagcgc tgggtaggca tcgagaccac gggcaggctg aagctagccg gtgtcgcgac
      421 ggcccttgag catgtggagc acggctgctt cctctgggtc cagtcgcttg cgcgatcggg
      481 tggtggcgat tgtctgccca tcgagatatg cctcgatgat ggccggatgg acgtagcacg
      541 cgcggcagac tgtgggagtg ttgccaagct gtgcggcgac tgccttgatc gcggcgacga
      601 cggtggcctc tgcctggcgc tcgtcgtccg aaggaccagc atcggccagt gcgccggccg
      661 ccaggaccgt tccggcccag gtgcggaagt ctttggcggt caagtcgcga cccgagatct
      721 gcttgagata ggcattgacg tcgtcggaac tgatcgtgtg gcgggttcct tcgtgatcga
      781 ggtactggaa tagttgctga cccggcaact cgcgcagcct cgcgatgaga cgcgctagcc
      841 ggcggtcgtg aataccgacg gtatgcttct tcgatccctt gccgttgaac tcgaagatga
      901 tgtcggcgcc atcgacctcg acatgacggt tgcgcatcgt cgtcagcccg aacgagtgat
      961 tgtcacgggc gtactccgtg ttaccgacgc gaatgtgggt gcttccgagg agcctgacaa
     1021 cgatcgccag cactttctcc ctcggcaagc ccggccgaga caggtcggaa tctacggtcg
     1081 cccggatcgc cggtaacgtc tcgccaaact cgatcatgcg gtcgaacttc gcttcatcgc
     1141 gaacgtcgcg ccagcgggga tggtagcggt actgcttgcg gttgcgggca tcccggcccg
     1201 tcgcctgaag atgtccgcgc ggcgagatac agatccagac gtctgtccag gcaggtggaa
     1261 tagccagtga cgcgatccga tcgagtgtcg ctggatcggt caggcgctcg cccgaagggt
     1321 cgtagtacga aaagcctttc ccacgccgaa cgcggcggaa acccggttcg gagtcattga
     1381 cgtaccgtag tccagcagca tgggcagctt cggtcgaatc atcctgactc gatccgttgg
     1441 tggatgatct ggctgattgc gtgctgctgg ttgaggttgt ggcaggcatt ctgacttccc
     1501 ggccgctaca tccggccagt cgattaggca cgtcgttccg gctgtcacag aatgcaatca
     1561 gagtgccagc gcacgtctag agtagactgg cactggagat gcgatgagat cggcgtggaa
     1621 ccagtcgtag cctgcgttga cggactggtc ggtttgcgat tagccttgtc aacggtacac
     1681 agacgccgcc ggatgatgcg ccacgcagcg atttcctgat gacacagggc gggtggttga
     1741 gtccccagcg atgagcgcag ctttgctggg cgaaggaggg tctggatggg gcaagtcatc
     1801 ttcgatcacg tcaccaaggc gttcgacacg ttcgtcgccg tcaaggactt cagcctggat
     1861 atccacgacg gtgagttcct tgtactggtc gggccgtccg gttgcggcaa gtcgacggcc
     1921 ctgcgctgcc tggccgggct cgaagagatc accagcggac gaatcttcat tggtgagcgg
     1981 gacgtgacct acctcgaccc caaggaccgc gatatcgcga tggtcttcca gaattacgcg
     2041 ctctacccac atatgtccgt ccgcgacaac atggcgttcg ggctcaagct gcgacggacg
     2101 tcgaaagagg tgatcgccca gcgggtgaac aacgccgccg agattctggg aatctcgcag
     2161 ttgctggatc gtaagccgcg ggcgctctcg ggcggccagc ggcagcgtgt cgcgctcggc
     2221 cgggcgatcg tccgtgagcc gcagtgcttc ctgatggatg aaccgctctc caaccttgac
     2281 gccaagcttc gtgttcagac ccgcgccgag atcatcaagc tccagaaacg gctcgggacg
     2341 acaacgatct acgtcaccca cgaccaggtc gaggcgatga cgatgggcga tcgcatcgcg
     2401 gtcatgaacg ccggcgagct gcagcagctc gacacgccgc agaatctcta caattcgccg
     2461 gctaaccgct tcgtcgcgac attcatcggc agtccggcca tgaatgtctt cccagcgatg
     2521 gtgtccaacg gcgccggcgc gccgatgatt gcggccggtg atttcaaggt ccaggtgccc
     2581 gccacgcggg cagatcgcgt gcgtgagcgc gccggccaga ctgtactggt tgggattcgc
     2641 cccgagcaca ttcaggtcag tgaatttgcc gaggacgtta ccgacggcaa caccatcacg
     2701 atgcccgtcg aactggtcga gcatcttggt gcggagacat tgctccacct gcgcgggccg
     2761 gaaggcgact cgatcgtcgc gagggtcgat cctcggacga cgctcaaggc aggcgacgtt
     2821 gccaaactag tggttaacac cggccagctg catgtctttg acccgacgac cgagcaggcc
     2881 cttactgccg agggcgcggc atcctcgtcc tgagctgcag cgaagcacct ctcctcatta
     2941 gccaaacagg cggttgaagc gggggaggcc cttcgctgcg gttcaggacg accgtttcgg
     3001 ctcattcagc aacgcatcga cctgtttgcg agtcggcaag ccaggttcgg cgccgcggac
     3061 ggtggctgcg tgcgctcctg ctgcacaggc gaagcgaacg gcatccgcca gccgttgccc
     3121 ctcgacgaag gccagcgcca gcgcggcgca gaagctgtcg ccagcggctg tcgcgtcgac
     3181 cgccgttacg ttgggcggtg ttgctcggcc gctcgacgtc ccgtccgacc agactgcacc
     3241 acgcgcgcca agcgtgacga cggcgatccg cccgtcggtc gcgagctttt gggcgaggtc
     3301 tagcggatcc gctctggacg ttgcgggtgt ctccagaagt gccaacgcct ctacctcgtt
     3361 cgcgacgatg atgtctgctg tcgcgatcaa cccggccgtg acgtgatgga cgggggctgg
     3421 attgagtatg acgatcgtgc cctggcgact ggcctgctgg gcgacggcga gcgacgtgtc
     3481 gagtgggatc tctccctgta gcagcaagac atcggcgtcg gggagatcgc ggcaatggtc
     3541 gggccgtaac tctgcgttcg ctccctgggc atggacgaat ccgacgtctc cgtcgggtgt
     3601 gacgagtggg actgcgatcg acgtgaagct ctgcggcaag cgaaccatcc gcaacgtgtc
     3661 gatgccctca cggtcgagcg acgcaatgaa ctcatcgccg taggcgtcgg cgccaagtgc
     3721 gccgatcatc gttacgctgg cgccgagccg ggcgagcgcg acggcctggt tgtagccctt
     3781 gccaccccga taagtgccga acgagtcggc caggatcact tcgccgggtt cggggcgttt
     3841 cggcacctga aagctcaggt ccatcaccag cgaaccgacg acgagcgccc ggaccgtgct
     3901 cattgtgcga cggccggttg taaggatttg tggcgagcca ccagctcggt tgtcgctggc
     3961 ggcttgcggg ctgtcaggtg caggccagat gccagcatcg cgccggagta ggcgcggtct
     4021 tccagatacg ccagccgcca ggtaacgacg aagatgccac gactgatgag cttgacggcc
     4081 gagaggggca gccggccggc tccacctgct gcgcgttcac gctgccaata gtcaacgatg
     4141 tcgtggacct ggccggccag gtggaacgaa tggctcaggc cggtgacggt gaagccggcg
     4201 tcccaggtca gcttgacgat gcgttgctcg tcaaagcgct ggatgtgacc gacgtgcttg
     4261 tgcttccagc ggtggatcgg gatcggcgag ttgcgccgca gcgctcggta gagcgtcttc
     4321 ggctggtctt cgagcgggac gaagaaatgg agcacgcaac ccggccgcag cacgcgcttg
     4381 cattcggcca gcagcgtcac cggcgacggg acgtgctcga tcaggtcgaa gaagatcacc
     4441 gcatcaaatg tgtcgctggc gaatggaatc ttctccgcat ccatgacgag gtaaagcggc
     4501 gtaccgccga tcgagacggc ttcggcaatc gcccggcggc taaggtctcc ggcgtagacg
     4561 tccagatcgg gccgagcgtt ggcgagcgca cgcgcatagc gacccgcgcc ggctccgagc
     4621 agcagcaggc ggccgccggt atcgccaagc gcgtcgagtg cccgctccag ccggatctcg
     4681 ccaaggttgc gggtgccagg gatgatctcg tctcggccct gaatcttggc cgggtcgtcg
     4741 atgtgatagc tgtcgcccat catcgtgtt
//
LOCUS       DASUGJ010000832         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2081719, whole genome shotgun sequence.
ACCESSION   DASUGJ010000832 DASUGJ010000000
VERSION     DASUGJ010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081719"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VFV93_18690"
     CDS             <1..1101
                     /locus_tag="VFV93_18690"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5167444.1"
                     /translation="DAGWDITVDASPSNPIDDLRGQLTGSDVDLDQIAAIGRSEGITY
                     ANSQVRNTGEQEWLNTTIRSLDSDFITNAEVPLEARAPGYNTDAAVWDALKAGENVAV
                     IDSTMLEGDPFGDSGNRYIVPDNVEVEDGSMPAFEVELYSARTGQTRTFTVIGLIDSQ
                     VSTMTGLYVPQPAFTELYDQPDLTRLYVRLRDHQAGDAGVVAKQIEAAMLNKGVQAES
                     IRDAIDEDASFANGFFQLLEGFMGLGLLVGIAALGVISFRSVVERRQQIGMLRAIGYQ
                     RRMVAASFLIESIVVAMLGVLSGTFLAIVLTYNLINGDALGEGQNFETFVIPWTTILF
                     FIAASLLAAAVMTWIPARKASSAPIAEALRYE"
     gene            1282..2196
                     /locus_tag="VFV93_18695"
     CDS             1282..2196
                     /locus_tag="VFV93_18695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5167445.1"
                     /translation="MDTNTFVIETRGLTRHYGEKIVAVDRLSLKVRRGEVYGFLGPNG
                     SGKTTTLRMLLGLTRPTSGTAEVLGEQPGTPTGLARIGAMIETPAFYPFLSGRDNLWV
                     MARHAGAPESRIADALEEVDLADRAGDRFSTYSLGMKQRLGVAAAILKDPELLILDEP
                     TNGLDPAGMAEMREFIRDLGQGRRTVLLSSHLMNEVEQVCDRVGVIRRGKLVAEGTVS
                     DLRGKPELRVRAQPLEAAHQVLSALPEIDEVEIVDGALRVAADPAVAPAVTRALVGAG
                     IDVNELVIRRSSLEDVFLHLTQDGEQQA"
     gene            2193..>3014
                     /locus_tag="VFV93_18700"
     CDS             2193..>3014
                     /locus_tag="VFV93_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167446.1"
                     /translation="MSHTSTRQRSRSIELPRTGAVAGGGFLSSLSAELFLLRKRSAYW
                     ILLALWTAVGTTFGFVVPYLTWRNNPETELSTFDALLPHSLVGNVAGGFPFYGGAFVL
                     ILGVLSIGSDYGWGTLKTTLTQRPGRMSYFTTKMVALALAIVPFVLSMYAVGGVASAL
                     NASRENAASNWPSLVSIVEGVATGWFILAVWGALGVMLAVLSRGTSLAIGIGILYGLA
                     LEGLLSALASQVSFLDPLVKFFLRANAYSLIQGFQGITQSDGPGAYRGPLVSTEQS"
BASE COUNT          505 a         1029 c          969 g          511 t
ORIGIN      
        1 gacgccggct gggacatcac cgtcgatgcc agcccgtcca acccgatcga cgatctgcgc
       61 ggccagctga ccggcagcga cgttgatctc gaccagatcg ccgccatcgg ccgcagcgaa
      121 ggcatcacct acgcgaactc gcaggtccgc aacaccggcg agcaggagtg gctgaatacc
      181 acgatccgct cactggattc ggacttcatc accaacgccg aggttccgct ggaagcgcgc
      241 gcgcccggct acaacaccga cgcagccgtc tgggacgcgc tcaaggcggg cgagaacgtc
      301 gccgtcatcg acagcacgat gctggaaggc gacccgttcg gcgacagcgg caaccgctac
      361 atcgtgccgg acaacgtcga ggtcgaggac ggctcgatgc cggccttcga ggtcgagctc
      421 tacagcgccc ggaccggcca gacgcgcacc ttcaccgtca ttggcctgat cgactcacag
      481 gtcagcacga tgaccggtct ctacgtcccg cagccggcct tcaccgagct gtacgaccag
      541 cccgacctga cccgcctcta cgtccggctg cgcgaccacc aggccggcga cgccggcgtg
      601 gtcgccaagc agatcgaggc ggcgatgctg aacaagggtg ttcaggccga gtcgatccga
      661 gacgcgatcg atgaggacgc ctcgttcgcc aatggcttct tccagctgct ggaaggcttc
      721 atgggcttgg ggctgctggt cggcatcgcc gcactgggtg tcatctcgtt ccgctcggtc
      781 gtcgaacgcc gccagcagat cggcatgctg cgggcgatcg gctaccagcg ccggatggtc
      841 gcggcctcgt tcctgatcga gtcgatcgtc gtcgcgatgc tgggcgtcct cagcggcacc
      901 ttcctggcga tcgtcctcac ctacaacctg atcaacggcg acgcgctcgg cgaaggtcag
      961 aacttcgaga cgttcgtcat tccctggacc acgatcctct tcttcatcgc cgcctcgctg
     1021 ctggcagcgg ccgtgatgac ctggatcccc gcgcgcaagg catcctcggc gccaatcgcc
     1081 gaagcgctgc gctacgagta gccagcgcca gcgatgctgc tgacacgggc cggtgggcaa
     1141 caacgcccgc cggcctgttt catgccgccg caagttgtaa cgcgctgttg accttctgtt
     1201 gagatctgcc cgaccggccg ttgatctcca tatctcacaa tcaaagggtg gtcgaacgcg
     1261 atcacaacga aaggagcctc gatggacacc aacacgttcg tgatcgaaac ccgcggtctg
     1321 acccgccact acggcgagaa gatcgtggcg gtcgatcgtc tctcattgaa ggtgcgccgg
     1381 ggcgaggtct atggctttct cggcccgaac ggctccggca agacgacgac gttgcggatg
     1441 ctgctgggcc tgacccggcc gacctccgga acggccgaag tactcggcga gcagcctggc
     1501 acccccaccg gcctggcccg gatcggggcg atgatcgaga cacccgcctt ctacccgttc
     1561 ctctcgggtc gagacaacct ctgggtgatg gctcgtcatg ccggcgcgcc ggagtcgcgg
     1621 atcgccgacg cgctcgaaga ggtcgatctg gctgaccggg ccggggaccg cttctcgacc
     1681 tactcgctgg gcatgaagca gcggctgggc gtcgcggcgg cgatcctgaa ggacccggag
     1741 ctgctgatcc tcgacgagcc gaccaatggc ctcgacccgg ccggaatggc cgagatgcgc
     1801 gagttcattc gcgatctggg gcagggacgc cggaccgtcc tgctctccag ccatctgatg
     1861 aacgaggtcg agcaggtctg cgaccgggtc ggcgtcatcc gacgcggcaa gctggtcgcc
     1921 gaaggcaccg tcagtgacct gcgcggcaag ccggaactgc gcgtgcgcgc ccagccgctg
     1981 gaggcagccc accaggtcct atcggccctg ccggagatcg acgaggtcga gatcgtcgat
     2041 ggtgccctgc gggtcgcggc cgatccggcg gtcgcgccgg ccgtcacccg cgcactcgtc
     2101 ggcgccggga tcgatgtcaa cgagctggta atccggagat cctcgctcga agatgtcttc
     2161 ctgcatctga cccaggacgg agagcaacag gcatgagtca tacatcgaca cgacagcgtt
     2221 cgcgatcgat cgagctgcca cggaccggcg ccgtcgcggg cggcgggttc ctcagcagcc
     2281 tctcggccga gctgttcctg ctgcgcaagc ggtcagccta ctggatcctg ctcgcgctct
     2341 ggacggcagt tggcacaacg ttcgggtttg tcgtcccgta cctgacctgg cgcaacaacc
     2401 cggagaccga attgtcaacg ttcgacgcgc tgttgccaca cagcctggtt ggcaacgtcg
     2461 ccggagggtt cccgttctac ggcggcgcgt tcgtcctgat tctcggtgtc ctcagcatcg
     2521 gcagcgacta cggctggggc acgctcaaga cgacgctgac ccaacggccg ggccggatgt
     2581 catacttcac gaccaagatg gtcgcgctgg cgctggcgat cgttccgttc gtcctctcga
     2641 tgtacgccgt cggcggggtc gccagcgcgc tgaacgcctc gcgcgagaac gcagcgtcca
     2701 actggccgtc cctcgtcagt atcgtcgaag gggtggcgac cggctggttc atcctggcgg
     2761 tctggggcgc actcggcgtg atgctggccg ttctctcgcg cgggacatcg ctggcgatcg
     2821 ggatcggcat cctctacggc ctcgcactcg aagggttgct gagcgcgctg gccagccagg
     2881 tcagcttcct cgatccgctg gtgaagttct tcctgcgcgc caacgcctac tcgctgatcc
     2941 aggggttcca gggcatcacc caaagcgacg gaccgggcgc ctatcgcggc ccgctcgtca
     3001 gtaccgagca gtcg
//
LOCUS       DASUGJ010000833         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1803859, whole genome shotgun sequence.
ACCESSION   DASUGJ010000833 DASUGJ010000000
VERSION     DASUGJ010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803859"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="VFV93_18705"
     CDS             complement(<1..1634)
                     /locus_tag="VFV93_18705"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX5167447.1"
                     /translation="MAGRRFFAGDRPIDGLARLALFAIALTVLSVSSVAVDWGSGDIV
                     VREGDAADQTIKAPETVTFDSELRTKEKRQAAYDDDANIALVNDESVRPEQVAAIDSF
                     LGRIDDLRADGRMTDEAKVAAIRDMLDGLSDNDAMDILSLSDTSWQRVRAEARRLVDV
                     TLANRLKPEDVARTKEELSRRVAALLSVDEQPIAAAIARPFIRANVFVDEEQTLRNRE
                     AAAEAVEPVQVQLLAGQAIVRDGDIVTVYDIEKLEHLGLLTQGTEPVVRLGKSGLMVL
                     LSLALVIYLYRFHRPIWQGRQLLLISIVVLGPILMARLVLPHDEIQYMFPVAASAMLL
                     AVLLEFPIALAISALLSLYLGVIANLSFELVVVYFIASAAGAFVIWRADRTMTFVWAG
                     LAVGVATFAAAICFAAANDALESASGAELLVEAVVNGALSASLTFLSFSLLGSLFGIT
                     THLQLLELAHPNQPLLYRLAREAPGTYHHSIVVSNLAESGVEIVGGDPLFARVAVLYH
                     DIGKIVRPTFFIENQANLENPHDLLDPRVSARVIID"
     gene            complement(1747..2208)
                     /locus_tag="VFV93_18710"
     CDS             complement(1747..2208)
                     /locus_tag="VFV93_18710"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX5167448.1"
                     /translation="MALTERLTEDMKSAMRSGDNRRRDVIRYLRAAITNAAIEKRTDL
                     DDSEIEQVIRTQVKQRRDSIEMFRNGGRDELADEEEAQVAILLEYLPAQLGHDELVEL
                     VRTTAESLGVSTQRDMSRLMPALVEAAGGRAEGRTLSQLAKEELARRSAAN"
     gene            complement(2362..2475)
                     /locus_tag="VFV93_18715"
     CDS             complement(2362..2475)
                     /locus_tag="VFV93_18715"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX5167449.1"
                     /translation="MVQDGYRVATNTGGWGGQSVDHLHLHLLGGRQLGTMG"
BASE COUNT          453 a          841 c          759 g          432 t
ORIGIN      
        1 tggtcgatga tgacccgcgc gctgacacgc ggatcgagca ggtcgtgcgg gttttccagg
       61 ttcgcctgat tctcgatgaa gaacgtcggc cggacgatct tgccgatatc gtggtagagg
      121 acggcaaccc tggcgaagag cggatcgccg ccgacgatct cgacaccact ctcggccagg
      181 ttgctgacga cgatcgagtg gtggtacgtc ccaggtgcct cacgcgccaa ccggtagagc
      241 agcggctggt tgggatgcgc cagttccagc agttgcaggt gcgtcgtaat gccgaacaac
      301 gagccaagca ggctgaacga caggaacgtc agcgaggccg acagcgcgcc gttgacgacc
      361 gcttcgacca gcagctcggc gcccgatgcc gattccagcg catcgttggc agccgcgaag
      421 cagatcgctg ccgcgaaggt cgcaacgccg acggccagcc cagcccagac gaacgtcatc
      481 gtcctgtcgg cgcgccaaat gacgaatgca ccggcggccg atgcaatgaa gtagacgacg
      541 accagctcga acgacagatt cgcgatgaca ccgaggtaga gcgacagcaa cgccgagatc
      601 gccagcgcaa tcgggaactc cagcaggaca gccaacagca tcgccgaggc cgccaccggg
      661 aacatgtact ggatctcatc gtgcggcagc accagccgcg ccatcaggat cggcccgagg
      721 acgacgatcg agatcagcag caactgccgt ccctgccaga tcgggcggtg gaatcgatac
      781 agatagatca ccagcgcgag gctgagcagc accatcagcc cggacttgcc cagccgaacg
      841 actggctcgg taccttgcgt cagcaagccg aggtgctcaa gtttctcgat gtcgtagacg
      901 gtgacgatgt cgccgtcgcg aacgatcgcc tgccccgcca gcagttgcac ctggaccggt
      961 tcgacagcct cagcagccgc ctcgcggttg cgcagcgtct gctcttcgtc gacgaagacg
     1021 ttggcgcgga tgaacggccg ggcgatcgcg gccgcgatcg gctgctcgtc gaccgacagc
     1081 aacgccgcga cgcgccgcga gagctcttcc ttcgtacgcg ccacatcttc cggcttgagc
     1141 cggttcgcga gcgtcacgtc gaccagccgg cgggcttccg cgcgaacccg ctgccaggac
     1201 gtatctgaca acgacaggat gtccatcgcg tcgttgtcgg agagaccgtc gagcatgtcg
     1261 cgaatggcgg ccaccttcgc ctcgtcggtc atacggccgt cggccctcag atcgtcgatc
     1321 cggccgagga aggagtcgat cgcggcgacc tgctccggcc tgacgctctc gtcgttgacg
     1381 agtgcgatgt tggcgtcatc gtcgtaggcc gcctgccgct tttccttggt gcgtagttca
     1441 ctgtcgaagg tgaccgtctc aggggccttg atcgtctggt cggcggcgtc accctcgcgg
     1501 acgacgatgt cgccggaacc ccagtcgacg gcgacgctgg agacgctcag cacggtcaac
     1561 gcgattgcaa acagcgcgag ccgggccagc ccgtcaatgg gccggtcgcc cgcaaaaaac
     1621 cgtcgaccgg ccaatcggct ccgccacgta ggctggatcc gctgtccgtt ggctactgcg
     1681 ctgtttcgct cgtcgctcat gttcgtggac ccgtcatatc cagacggttg ccgcccgccg
     1741 gtgcgctcag ttggcggccg atcgacgcgc cagctcttct ttcgcgagct ggctcagcgt
     1801 gcgtccttcg gcccggccac cggccgcttc gacgagcgcc ggcatcaacc gcgacatgtc
     1861 ccgctgggta gagacgccaa gcgactcggc cgtcgtgcgc acgagttcca ccaactcgtc
     1921 gtgaccgagc tgcgccggaa gatactcaag caggattgca acctgcgctt cctcttcgtc
     1981 tgcaagctcg tcacgaccac cgttgcggaa catctcgatc gagtcccggc gctgcttgac
     2041 ctgcgtccgg atcacctgct cgatctcgga atcgtccaga tcggtgcgct tttcaattgc
     2101 cgcgttggtg atcgccgccc gcaggtaacg gatcacgtcg cgccggcgat tgtcccccga
     2161 gcgcattgcc gatttcatgt cttcggtcag acgttctgtc aacgccaatg gttccgaccc
     2221 cttgctagcc gtcggtatca caacggcatc taaccattgt acgcgcgcgg caggctgtcg
     2281 gttccggcta tctaaacaca gccgccacca cgattcaacg cagacgcctg gccagacccg
     2341 cgtacgtgcg cgtctttcgg gctagcccat cgtccccaat tgccgtccgc cgagcaaatg
     2401 caggtgcaga tgatcgaccg actggccgcc ccagccaccc gtgtttgtcg caactcggta
     2461 gccatcctga accagcccct cactg
//
LOCUS       DASUGJ010000834         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_691526, whole genome shotgun sequence.
ACCESSION   DASUGJ010000834 DASUGJ010000000
VERSION     DASUGJ010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691526"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="hpt"
                     /locus_tag="VFV93_18720"
     CDS             <1..786
                     /gene="hpt"
                     /locus_tag="VFV93_18720"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX5167450.1"
                     /translation="TPDFYLPERDIFVELTTMKQSLVTRKNRKIRRLRELYPNVNVML
                     VDRKAFHEILGRFGYGSVDITSLRQEDIDRILLSSSEIQQRVVDLGKEISDDYAGQSL
                     VLVGLLKGVTFFLADLARAVTRPLAIDYLSVKAPLHSPDGITVERHLDIDIHDKHVLL
                     VEDIVNTGLTMDFVLDYLRDQRPASVAVCALLDRVERRIAPVDVRYVGFSIPNDYIVG
                     YGLDHHELYRNLPFICVLKREAYEQHNIADESASQYLESQAHQ"
     gene            783..2096
                     /locus_tag="VFV93_18725"
     CDS             783..2096
                     /locus_tag="VFV93_18725"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5167451.1"
                     /translation="MSRHLRLLVMLMAVVSVLAGACSSSGDDEPDETATSPTGPAATP
                     AQGPATATQSSAAATHPVEISPTSEASPTTSGNATVPAATEPPVPEGTATVSVGDQTL
                     TVPAGYSISIYAEDLGAARFMALDEDGTVYVADRRGRVIRLPDNDDDGVADDVETVLD
                     GLDTPHGITFHDGALYVAEQTRVIRATDEDSDGTFESVTTIIDGLPEGGHSTRTIRFG
                     PDGMLYVSIGSSCNVCDEEDSRRAAIMRYNADGSGGEPFAIGLRNAVGITFDNETGLL
                     WATNNGRDGMGDNVPPETINQPNQGDDFGWPRCHAGDVIDPQFGSEDACDGVAPPAVK
                     MQAHSAPLGLTFVSGDQLAQYAGDLLVAFHGSWNRSEPTGFKVVRVPFESGVPAGAVV
                     DFISGWLLPDGERWGRPVDVLIAPDDSLLISDDEGGRIFRLHRST"
     gene            2224..3033
                     /locus_tag="VFV93_18730"
     CDS             2224..3033
                     /locus_tag="VFV93_18730"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX5167452.1"
                     /translation="MAKRVEDDFELTRQLIVEFIGTFALMFFGGGAIIVTAAEGLGAN
                     AGLLVVALAHGLAIGLMVAAAGHISGGLYNPALTVGLMVTGRMPSVRGVAYIVTQCVG
                     SLIAALALKAIFDASQIDAVKLGVPLVGARYEVTAALLAEIIATFFLMYAVFGTAVDP
                     RGAKSIAGLVIGLTITIGVLAIGPVSGAALNPARAIGPEIAQGEFGDFWIYWVGPIGG
                     AALAALLYNYILMPGAITPEAGRVESEPTEAHNIERESRSQPRPQQRRRKR"
BASE COUNT          539 a          979 c          975 g          565 t
ORIGIN      
        1 acgcccgact tctacctgcc cgagcgcgac atcttcgtcg agctgacgac gatgaagcag
       61 tcgctcgtca cccgcaaaaa ccgcaagatc cgtcgcctgc gcgagctgta cccgaacgtc
      121 aacgtcatgc tggtcgatcg caaggcgttc cacgagatcc tcggccgctt cggctacggc
      181 tcggtcgaca tcacctcgct gcgtcaggag gacatcgacc gcattctgct ctcgtcgtcg
      241 gaaattcagc agcgcgtcgt cgacctcggc aaggagatct cagacgacta cgctggtcaa
      301 tcgctggtgc tggtcggtct gttgaagggc gtcacgttct ttctggcaga cctcgcccgc
      361 gcggtaaccc gcccgctggc gatcgactat ctctcggtca aggcgccgct ccactcgccg
      421 gacggaatca cggtcgagcg acatctcgat atcgacattc acgataagca cgtcctgctc
      481 gttgaggata tcgtcaacac cggtctgacg atggacttcg tgctggacta cctgcgggac
      541 caacggccgg ccagcgtcgc cgtctgtgcg ctgctggacc gcgtcgagcg ccggatcgcg
      601 ccggtcgatg tacgctacgt cggcttcagc atcccgaacg actacatcgt tggctatggc
      661 ctcgaccacc acgagctgta tcgcaacctg ccattcatct gcgtcctcaa gcgcgaagcc
      721 tacgagcagc acaatatcgc cgacgaatcg gcctcgcagt atctcgagag ccaggcgcac
      781 caatgagccg acacctgcgg ctgcttgtca tgttgatggc ggtcgtctcg gttctggctg
      841 gtgcatgcag ctcgtccggc gacgacgagc cggatgaaac tgcgacatcg ccgaccggcc
      901 cagccgcgac cccggcgcag ggacctgcga ctgcgacaca atccagcgcc gcggcgacgc
      961 atcctgtcga aatcagcccg acctccgagg caagtccaac cacgtcaggc aatgccaccg
     1021 tgccggctgc taccgagcca cctgtacccg aaggcacagc aaccgtctcg gtcggcgacc
     1081 agacgctgac agtgccggcc ggctactcga tctcgatcta tgccgaggat ctcggcgcgg
     1141 cccgcttcat ggccctcgat gaagacggta ctgtctacgt tgccgatcgg aggggccgtg
     1201 tcatacggct gcccgataac gatgacgatg gtgtcgccga cgatgtcgag acggtactgg
     1261 acggactcga taccccgcat gggattacct tccacgacgg agccctctac gtcgccgagc
     1321 agacgcgtgt tattcgcgca accgacgaag atagcgacgg gacgttcgag tctgtcacga
     1381 cgatcatcga cggcctgccc gagggcggtc attcgactcg caccattcgt ttcggcccgg
     1441 acgggatgct gtatgtctcg atcggctcct cctgcaacgt ctgcgatgag gaggattccc
     1501 ggcgcgcggc aatcatgcgc tacaacgcgg acggcagcgg cggagagccg ttcgcgatcg
     1561 gtctccgtaa tgcggtcggg atcacgttcg acaatgagac agggttgctc tgggcgacca
     1621 acaacggccg cgacggcatg ggtgacaacg tcccgcccga gacgatcaac cagcccaacc
     1681 agggtgacga cttcggctgg ccgcgctgcc acgccggcga cgtgatcgat ccgcagttcg
     1741 gcagcgagga tgcctgcgac ggcgtcgcgc cacccgccgt caagatgcag gcgcattcgg
     1801 cgccgctcgg cctgacgttc gtcagcggcg accagctcgc gcagtacgcc ggcgatctgc
     1861 tggtcgcgtt ccacgggtca tggaaccgtt cggagccgac tggcttcaag gttgtccggg
     1921 tgccattcga aagcggcgtg ccggcgggcg cggtcgtcga tttcatttcc ggctggctgt
     1981 tgccggatgg tgagcgctgg gggcgccctg tggatgtcct gatcgctccc gacgatagcc
     2041 tgctgatctc agacgatgag ggtggccgga tcttccggct ccatcgctcg acataacaag
     2101 ctaaacaacc agcatcggcg cgttcgtgtc gcttgatcca tcgcggatgc ggcactacac
     2161 tgccctacgc gggagcatca gacgtgggtg gccgcgatcg cggcgagaag gggatgggga
     2221 ctcgtggcta aacgggttga agacgatttt gagttgacgc gccaactcat cgtcgagttc
     2281 atcgggacgt tcgcgctgat gttcttcggc ggcggcgcga tcatcgtgac ggcggcggaa
     2341 gggttggggg caaacgcggg tctgctcgtg gttgcgttgg cgcacggcct ggcgattggg
     2401 ctgatggtgg ctgctgccgg gcacatctcc ggtggtctct acaacccggc cctgacggtc
     2461 ggcctgatgg tgacgggcag gatgccgtcg gttcgtgggg tcgcatatat cgtcacccag
     2521 tgcgtgggat cgcttattgc cgcgctggcg ttgaaggcga tcttcgacgc atcacagatc
     2581 gacgccgtca agctcggcgt gccgctggtc ggtgcgcgct atgaagtcac cgccgctctg
     2641 ctggcggaga tcatcgcgac cttcttcctg atgtacgccg tcttcgggac agccgtggat
     2701 ccgcgcggcg cgaagtcgat cgcgggactg gtgatcggac tgacgatcac gattggcgtt
     2761 ctggcgatcg gcccggtcag cggtgccgct ctcaatccgg cccgtgcgat cggaccggaa
     2821 attgcccagg gcgagttcgg tgacttctgg atctactggg ttggaccaat tggcggtgcc
     2881 gcgctggccg cactgctcta caactacatc ctgatgccgg gggcaatcac gccagaggca
     2941 ggccgagtcg aatccgagcc gaccgaagca cacaacatcg agcgtgagtc tcgcagccag
     3001 ccccgccccc aacagcgccg tcgcaagcgg taacggcagt ggctcggtag ttcatcac
//
LOCUS       DASUGJ010000835         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1666019, whole genome shotgun sequence.
ACCESSION   DASUGJ010000835 DASUGJ010000000
VERSION     DASUGJ010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666019"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /locus_tag="VFV93_18735"
     CDS             complement(<1..1791)
                     /locus_tag="VFV93_18735"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5167453.1"
                     /translation="MLILPVLAACGGDDDDDAEPTNTAAAAAATNTTSSGAGEATATE
                     GEGEASPTEGEAASPTTGGTDASPSTGTGSTGGGQTFGPFTPEDLMFGYENEEPESEG
                     GVLVEGNALDVSTLLPIVTQDVPSSDVQGMIFESLVTVNPDTLEAVGLLAESWDVNAD
                     ASVYTFHLRDGVNWSDGEPFTAEDVEFTYNTYMNPDTGSPRVSDMTIQIDSIEVLDPM
                     TVKFNLKGAFPDFPIDLGGYNLIAKHIWGDIDAAAMQADPGATGTDPERVVGTGPFLF
                     KEWITGDHVTLIPNPNYWNGVAILDEYIFKILPDDSAAVNQLKTGEIDWAALTNSAVS
                     EFDGNQDVSVVDFPQLSFSFYGLNLNADTTTLFQDVNVRQALFYALDREAMVDSIYFG
                     FAEVAIGTIPSMSWAYNPDEIEMKYPYDPEMAKQLLDEAGWTDTNGNGTRDKDGQELA
                     FTMYGISGNAVAEASLVAMQEFWSEIGVDMTPAPEPFPALVERITETFDFEAFLVGFS
                     WGPAVDQSTMFTCDAAGGNGFNITSYCNPEVDAIMKEALSEPDQAKRIELYTEFQNIV
                     SVDLPETVMFFARGINGLANRVHNVHPNQVN"
     gene            complement(1998..>2757)
                     /locus_tag="VFV93_18740"
     CDS             complement(1998..>2757)
                     /locus_tag="VFV93_18740"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5167454.1"
                     /translation="LEQLVAGWGSGAVRVVDLTQPLDTATPVIGLPEIFAPSPGLSVE
                     EISRYDERGPGWYWNTIRLGEHTGTHFDAPIHWVTGKDLPGNACDTIPPGRFVGPACV
                     LDFSAESAADPDFLLLPEHISAWEERFGRIPAGSWALFRTDWSKRTDPAAFFNADENG
                     PHTPGPHQSAVELLIERDVLGFGVETVGTDAGQAGMFDPPFPCHTFMHGAGKFGLASL
                     ANLDQLPPVGAVVIAAPLKLVGGSGSPVRVLALV"
BASE COUNT          500 a          916 c          838 g          503 t
ORIGIN      
        1 gttaacctgg ttcgggtgca cattgtgcac ccgattggcc aggccgttga taccgcgggc
       61 gaagaacatc accgtctctg gcaagtctac agacacgatg ttctggaact cggtatagag
      121 ttcgatgcgc ttcgcctgat ctggctcgct gagcgcctcc ttcatgatcg catcgacctc
      181 cggattgcag tagctggtga tgttgaagcc gttgccgcct gcggcgtcgc aggtgaacat
      241 cgtcgactga tcgacggccg gaccccaact gaatccaaca aggaacgcct cgaagtcgaa
      301 tgtctcggtg atccgctcga ccagcgccgg gaatggctct ggcgccggcg tcatatcgac
      361 gccgatctcc gaccagaatt cctgcatcgc gaccagcgag gcttctgcga cggcgtttcc
      421 ggagatgccg tacatcgtga aggccaactc ctggccgtcc ttgtcacgag tgccgttgcc
      481 gttcgtgtcg gtccagccgg cttcatccag cagctgcttg gccatctcag gatcgtacgg
      541 atacttcatc tcgatctcat cagggttgta cgcccatgac attgacggga tcgttccgat
      601 agcgacctcg gcgaatccga agtagatcga atcgaccatg gcctcacggt cgagtgcgta
      661 gaacaacgcc tggcggacgt tgacatcctg gaagagcgtc gtcgtgtcag cgttcaggtt
      721 gaggccgtag aacgagaagg agagctgtgg gaagtcgacg accgatacat cctggttgcc
      781 gtcgaactca gaaacggcag agttcgtcag cgcggcccag tcgatctcgc cggtcttgag
      841 ctggttcacg gccgcagagt cgtcgggcag gatcttgaag atgtactcat caaggatcgc
      901 aacgccattc cagtaattgg ggttcgggat aagcgtcacg tggtcaccgg tgatccattc
      961 cttgaagagg aacggaccgg tgccaacgac gcgctccgga tcggttccgg tcgcgcctgg
     1021 atctgcctgc atcgccgccg catcgatatc gccccagatg tgcttcgcga tcaggttgta
     1081 gccgccgagg tcgatcggga agtccgggaa ggcgcccttc aggttgaact tcaccgtcat
     1141 cgggtccagc acttcgatgc tgtcgatctg gatggtcatg tcgctgacac gcggcgaacc
     1201 ggtgtccggg ttcatgtacg tgttgtaggt gaactcaacg tcctcggccg tgaacggttc
     1261 gccgtctgac cagttgacgc cgtctcggag atggaacgtg tagaccgagg cgtctgcatt
     1321 cacatcccac gactcggcca gcagaccgac tgcctccagc gtgtccggat tgaccgtgac
     1381 gagcgattcg aagatcatgc cctggacgtc gctagacggt acgtcctggg tcacgatcgg
     1441 cagcagcgtc gagacgtcca gcgcgttgcc ttcgacaagg acgccgccct cgctctccgg
     1501 ctcttcgttc tcgtagccga acatgaggtc ttccggcgtg aacggaccga aggtctgccc
     1561 accaccggtc gagccggtgc cggtcgatgg cgatgcgtcg gttccgccag tcgtcggcga
     1621 ggcggcttcg ccctcagtcg gcgatgcttc accctcgccc tcggtcgccg tcgcctcgcc
     1681 tgcgccggac gaggttgtgt tggttgccgc cgcggcggcc gccgtgttgg tcggctcagc
     1741 gtcgtcgtca tcgtcaccgc cgcaggccgc gagcactggc aggatcagca ccagtaccat
     1801 caacgcggcg atgagtctcc acctccgtaa atgattaccc ctgagcattc tccatccttc
     1861 caaccagccg gtaaccgctg cgactacatc cccctgatgc gcagcgccag ccatcagcgc
     1921 actgacgcaa ccgccagtgc cgcgatagta tccagcctat cgatcaagcg cgcaatgcaa
     1981 caggtgaaac tcccggccta gaccagcgcc aggacgcgta cagggctgcc actgccaccg
     2041 accagcttga gcggcgccgc gatcacgacc gcgccaaccg gcggcagctg gtcgagattc
     2101 gccagcgagg cgaggccgaa cttgccggcc ccgtgcatga acgtgtgaca cgggaacggc
     2161 ggatcgaaca tccccgcctg accggcgtcg gtcccgaccg tctcgacgcc aaacccgagc
     2221 acatcgcgct cgatcagcaa ctcaaccgcc gactgatgcg gccccggcgt gtgcgggccg
     2281 ttctcgtcgg cgttgaagaa ggcagccggg tcggtccgct tgctccagtc agtccggaat
     2341 agcgcccagc tcccggccgg aatccgccca aagcgctcct cccaggcgct gatgtgctcc
     2401 ggcagcagca gaaagtcggg atcggccgcc gactctgccg agaagtccag cacgcaggcg
     2461 ggcccgacga agcggccggg cgggatcgtg tcgcacgcgt tgccaggcag gtccttgccg
     2521 gtcacccagt ggatcggcgc gtcgaagtgc gtcccggtgt gctcgccgag ccggatcgtg
     2581 ttccagtacc agcccggccc gcgctcgtca tagcgcgaga tctcctcgac cgacaacccc
     2641 ggcgacggcg cgaagatctc cggcaagcca atcaccggcg tcgccgtatc cagcggctgc
     2701 gtcaggtcaa cgacccgcac cgcccccgac ccccaccccg ccaccaactg ctccagc
//
LOCUS       DASUGJ010000836         4707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_2081868, whole genome shotgun sequence.
ACCESSION   DASUGJ010000836 DASUGJ010000000
VERSION     DASUGJ010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081868"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFV93_18745"
     CDS             complement(<1..322)
                     /locus_tag="VFV93_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167455.1"
                     /translation="MDDRTQRIVDALVACGQRVREVVVRAEYHRLAEVVGLGADGADT
                     THIDQVAEDVALACLEASEPRMNILSEEIGFLDRGSSLTAIVDPIDSTNNAIAVPNFV
                     RQPGA"
     gene            496..1464
                     /locus_tag="VFV93_18750"
     CDS             496..1464
                     /locus_tag="VFV93_18750"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5167456.1"
                     /translation="MARHDAGLATDYWKRYFTPEEVAEIVDSSTTTTLVSDGLPIHVR
                     LYERSRDAPTVIMSHGLITYGLALARLQLPFYRAGYNVVQWDMPGWGQSGGPRGGCTI
                     DQATDTWSVVLDWVASEFDGPLYTTGFGEDGTTCYYALANDPRIRAMAFHNLWDYTDP
                     ELMRWQGSAPVIRAKRGILFFARYLLPTRTVDMRIAVPWDDLFGKEEDTEYRRIFEGD
                     PLRNKGYQYPLAYSMLKNHRPRAPFEACRTPIMLIVSELNELWPLSMNQRTFDRLGGE
                     KEMIVLEGKPHWEFKREFDETFCVHAMGWFARHGAVEASTPSAAAS"
     gene            1477..3003
                     /locus_tag="VFV93_18755"
     CDS             1477..3003
                     /locus_tag="VFV93_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167457.1"
                     /translation="MSRTTTIALLIAFCICGTLTTLAFYWDEAWHTDKGRDSFWIPAH
                     QMLYGSVAGLIVIVAIWSAILSYNQVGWRDIVRHWPLALAFTGAAVTLMDAPIDGIWH
                     NTFGRDAVFWSPPHVLAVAGLTMTASGMLLKASGLGGRAGDILTVMTAALVMSASVVL
                     VMEYDSDVPQFSTLWYLPVIAMTATFTLTLAGSVSPLRWIGTLTSLTYAGIMTAVFFY
                     LSAVLDSTPIIPPLVIPGLVFDLARRYKLPLPVRGTLYSVALYATYTPYLNRMLNGVS
                     LSTSDVLIGLPLAAIISTLVLYAFGASLPRPAIRLSPSSAAVTAGLLLALLPALAFAH
                     DPGQGPKVGNAELTATQTDGQLSVTGQIVDPDPCDVFTARGLVARRARQEVRGTLQQT
                     GDCTFTGQLTLPERGRWFVYLELNQSGKGVEVWLPVMSRDGTRTFEKESWIYIPPTRK
                     VNAISITAGILIYALSIALITTVGIGFRRTRARRDAGILDPADSPPLRVRQEQPAPAT
                     "
     gene            3110..3301
                     /locus_tag="VFV93_18760"
     CDS             3110..3301
                     /locus_tag="VFV93_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167458.1"
                     /translation="MPRHVLTMIGIVGAFFFLLWFGVVVLGQSAGEVFVASVATLFLV
                     SLGVTFSVSSLRSERRDQE"
     gene            complement(3317..3976)
                     /gene="phoU"
                     /locus_tag="VFV93_18765"
     CDS             complement(3317..3976)
                     /gene="phoU"
                     /locus_tag="VFV93_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX5167459.1"
                     /translation="MTRTVFTQSLQDLQDGVLTLGSMVEKAIGRAVESLRNQDVVLAQ
                     QVVDEDANINDVRWELEEAAINLMATQAPMARDLRRIAAVMHIVTELERMADHAEGIA
                     VLTLRTADEPHLKPLVDIPKMADIAREILIEALDAYTEMDAERARQIARRDDEVDRLY
                     EQVYRELLTFMISDPRTITRATHLLWVAHNVERIADRVTNICERVVFVVTGQIEEMNR
                     K"
     gene            complement(4074..>4707)
                     /locus_tag="VFV93_18770"
     CDS             complement(4074..>4707)
                     /locus_tag="VFV93_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX5167460.1"
                     /translation="TLLRCFNRMNDLVPSARVDGRILFHGENLYDSDIDPVAVRRLSG
                     MVFQKPNPFPKSIYDNVAWGARINGFKGNMDELVERSLRSAALWDEVKDKLKSSGLSL
                     SGGQQQRLCIARCIAIQPEVILMDEPCSALDPIATLRIEDLMRELCENYTILIVTHNM
                     QQAARVSDMTAFMTMGEGRAGELVEVGPTRELFTSPKNPRTEDYITGRVG"
BASE COUNT          790 a         1586 c         1454 g          877 t
ORIGIN      
        1 tggctccggg ctggcggacg aagttgggga cggcgatcgc gttgttggtc gagtcgatcg
       61 ggtcgacaat cgccgtcagc gacgagccac gatcgaggaa gccgatttct tccgagagga
      121 tgttcatacg tggctcgctc gcttcgaggc aggccagcgc cacgtcctcg gcgacctggt
      181 cgatgtgggt cgtgtctgcg ccatcggcgc ccagcccgac gacctcggcc agccggtggt
      241 attcggcgcg aacgacgact tcgcgaaccc gctggccgca cgccaccaac gcatccacga
      301 tccgctgcgt cctgtcatcc atctgcatcg agttcgtcaa cctttgcgat tcggccggtt
      361 aggtggtcgg tagcattccc cgcctgccgc tcacgttgac gaacgacggg atcagcacta
      421 cagtgaggat acgcgaacca accacttgaa tccgcatcca gtccttgcca atttgcaata
      481 gcctggagaa tgccaatggc gcgccacgat gcagggctcg caaccgacta ctggaaacgc
      541 tacttcacgc ccgaagaagt cgctgagatc gtcgactcca gcacgacgac gacgctcgtg
      601 tcagatgggc tgccgatcca cgttcggctc tatgaacgct cgcgggatgc cccgaccgtc
      661 atcatgtcgc atggcctgat cacctacggg ctggcactgg ctcggctcca gttgccgttc
      721 taccgcgccg gatacaacgt cgtccagtgg gacatgccag gctgggggca gagcggcggg
      781 ccacgcggcg gctgcacgat cgaccaggcc accgacacct ggagcgtcgt gctcgactgg
      841 gtcgcatccg agttcgacgg accgctctac acgaccggct tcggcgagga tgggacgacc
      901 tgctactacg cgctcgccaa cgacccacgc atccgcgcga tggccttcca caatctctgg
      961 gactataccg accccgaact gatgcgctgg cagggctcgg cgcccgtcat ccgcgccaaa
     1021 cgcggcatcc tcttcttcgc ccgctacctg ctgccgacgc ggacggtcga tatgcgtatc
     1081 gccgtcccgt gggacgatct gttcggcaag gaggaagaca ccgagtaccg cagaatcttc
     1141 gagggcgatc cgctgcgcaa caagggttac cagtacccgc tggcctactc gatgctcaag
     1201 aaccaccggc cacgggcgcc gttcgaagcc tgccggacgc cgatcatgct gatcgtctcc
     1261 gagctgaacg agctctggcc gctcagcatg aaccagcgca ccttcgaccg gctcggcggt
     1321 gagaaagaga tgatcgtgct cgaaggcaag ccgcactggg aattcaagcg cgaattcgac
     1381 gagaccttct gtgtccatgc catgggctgg ttcgcgcggc acggcgccgt cgaggcgagc
     1441 acgccgagcg cggcagccag ctaggaggtt gcgcccatgt cgcgaacgac gacgatcgcc
     1501 ctcctgatcg cgttctgcat ctgcggcaca ctgacgaccc tggcgtttta ctgggacgaa
     1561 gcctggcata ccgacaaggg ccgcgacagc ttctggatcc ccgcccatca gatgctgtat
     1621 ggctcggtcg ccggcctgat cgtcatcgtc gccatctggt ccgcaattct gtcgtacaac
     1681 caggtcggct ggcgtgacat cgtgcgccac tggccgctgg cgctggcgtt caccggcgcg
     1741 gccgtgacgc tgatggatgc accgatagac ggcatctggc acaacacctt cggccgggac
     1801 gccgtcttct ggagcccgcc gcatgtcctg gcggtggccg ggctgacgat gacagcctcc
     1861 gggatgctct tgaaagcgag cggcctcggc gggcgggccg gcgacatcct gaccgtcatg
     1921 actgccgcgc tggtgatgtc cgcctcggtc gtactcgtca tggaatacga ctccgacgtg
     1981 ccgcagttct caacactctg gtacctgccg gtcatcgcga tgacggcgac cttcacgctg
     2041 acactggccg gcagcgtctc gccgttgcgc tggatcggga cactgacctc gctgacctac
     2101 gccgggatca tgacggccgt cttcttctac ctgagcgccg tcctcgactc gacgccgatc
     2161 atcccgccac tcgtcatccc cggcctcgtc ttcgacctcg cgcgccgcta caagctgccg
     2221 ctgccggtac gcggcacgct ctacagcgtc gcgctgtatg cgacctacac gccctacctc
     2281 aaccggatgc tcaacggcgt cagcctctcc accagcgatg tcctgatcgg attgccactg
     2341 gccgccatca tcagcacgct ggtgctgtac gcgttcggcg ctagcttgcc ccggccagcc
     2401 atcagactct cgccgagcag tgcggcggtc actgccgggt tgctgctggc cctgctcccg
     2461 gcgctcgcct tcgctcacga tccgggtcag gggccaaaag tcggcaacgc cgagctgacg
     2521 gcaacccaga ccgacgggca gctcagcgtg accggtcaga tcgtcgatcc cgacccgtgc
     2581 gatgtcttta cggcgcgcgg tctcgtcgcc cggcgagcgc gccaggaagt gcgcggcacg
     2641 ttgcagcaga ccggcgattg cacgttcacc ggtcagctga cgctacccga acgcgggcgc
     2701 tggttcgtct acctggagct taaccagtcc ggcaaggggg ttgaggtctg gctgccggtc
     2761 atgtctcgcg atggtacccg cacgttcgag aaggagagct ggatctacat cccaccgacg
     2821 cgcaaggtca acgccatctc gatcacggct ggcattctca tctacgcgct ctcgatcgcg
     2881 ctcatcacga ccgtcggcat cggctttcgc cgcacccggg cgcgccggga cgcgggcatc
     2941 ctcgatccgg ccgacagccc gccactgaga gtcagacagg aacagccggc tcccgccacc
     3001 tgatcgcgcc agatcgcggc gttccaccac cagcatcggg actcctccga ccagggcgct
     3061 tgccgacgtc gatttgttat cgtaacgacg ggaggaggaa ccgacgttta tgccgcgaca
     3121 tgttctcacg atgatcggga tcgtcggcgc gttcttcttc ctgctctggt tcggcgtcgt
     3181 cgtcctgggt cagtcggccg gcgaagtatt cgtcgccagc gtcgccaccc tcttcctcgt
     3241 cagcctcggc gtcaccttca gcgtctcgtc gctgcgtagc gaacgccgcg accaggagta
     3301 gccgcctgcc gggcagctac ttgcggttca tctcctcgat ttggccggtg acgacgaaga
     3361 cgacccgctc gcagatattg gtgacgcgat cggcgatgcg ctcgacgttg tgggccaccc
     3421 agagcagatg ggtcgcgcgg gtgatcgtgc gcgggtcgga gatcatgaac gtcagcagct
     3481 cgcggtagac ctgctcgtag agacggtcga cctcgtcgtc acggcgggcg atctggcggg
     3541 cgcgttcggc gtccatctcg gtgtaggcgt ccagtgcctc gatcaggatc tcgcgggcga
     3601 tatcggccat cttgggaatg tcgaccagcg gcttgaggtg cggctcgtcg gccgttcgca
     3661 gcgtcaggac agcgatcccc tcggcgtgat cggccatccg ctccagctcg gtgacgatgt
     3721 gcatgacggc cgcgatccgg cgcagatcac gcgccatcgg cgcctgggtc gccatcaggt
     3781 tgatggccgc ctcctccagc tcccagcgca cgtcgttgat gttggcgtcc tcatcgacca
     3841 cctgctgggc cagcacgaca tcctgattgc gcagcgactc aaccgcccgg ccaatcgcct
     3901 tctcaaccat gctgccgagc gtcagcacgc cgtcctgaag atcctggagg ctttgggtga
     3961 aaacggtgcg ggtcatggcg tctccttcgg tcgggattcg ggactcggga ttcgggagtg
     4021 ttgaacagcg tcttttccct tgacactccc ggttcccgaa tcccgaatcc cgttcatccc
     4081 actcgcccag taatgtaatc ctcagtccgt gggttcttcg ggctggtgaa cagttcgcgg
     4141 gttgggccga cctcgaccag ttcgccggcg cggccctcgc ccatcgtcat gaaggcggtc
     4201 atgtcggaga cacgcgcggc ctgctgcatg ttgtgcgtca caatcagtat cgtgtaattc
     4261 tcacacagtt cgcgcatcag gtcttcaatt cgcaacgtcg caatcgggtc gagggcggag
     4321 caaggctcgt ccatcaggat cacctccggc tggatcgcaa tgcagcgcgc gatgcagagg
     4381 cgctgctgct ggccgccgga gagagacagc ccgctcgact tgagcttgtc cttgacctca
     4441 tcccagaggg cggccgagcg caggctgcgc tcgaccagct cgtccatgtt gcccttgaag
     4501 ccgttgatcc gcgcgcccca ggcgacgttg tcgtagatcg acttcgggaa cgggttcggc
     4561 ttctgaaaga ccattccgct caagcggcgg acggcgaccg gatcgatgtc ggaatcgtac
     4621 agattctcgc cgtggaacag aatgcggcca tcgacccgcg cgctcggcac gaggtcgttc
     4681 atccggttga aacagcgcag cagcgtg
//
LOCUS       DASUGJ010000837         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_414073, whole genome shotgun sequence.
ACCESSION   DASUGJ010000837 DASUGJ010000000
VERSION     DASUGJ010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414073"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VFV93_18775"
     CDS             <1..1054
                     /locus_tag="VFV93_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167461.1"
                     /translation="DDDQTPAIGPSGPTPTVEVTETPAAVVPAPTEPATATTEPAATA
                     TATAEPSPTPSLPSFGTIAATIPVGAGVRNMAAGEGALWVTNASDGTVSRIDLATNEV
                     VATIEVAPPGDQSVDAIAVGGGAVWVTTQGSIQLARIDPATNQVVTTISLNTAVHELV
                     FGGGALWGRGSFSNTIVRVDPATNTVVASIDVPPPTAIVYLDDALWVMDVSDVSKIDP
                     ATNSIVATVHYNDDPSGPTGTGIVAAGGSLWLSDDSLGPELVQIDPASATILARVALP
                     VEVDRIATAGSADAVYICGCTGSPDTMIWQFDVATHELTAALNVPDAEWLISDADSLW
                     TYNGPLQEVTRVDVTS"
     gene            1089..1655
                     /locus_tag="VFV93_18780"
     CDS             1089..1655
                     /locus_tag="VFV93_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167462.1"
                     /translation="MTRILRRLILRLVALQAVLLLVLTPTALAANPTPGLTPPPPSDA
                     ICSTSPHGTICHVDGAFTREDPELTVCDNVVVDGSSSGTASRKLTYDATGALVKEQRH
                     VRFTGTLTNRSSGESIGYAGTFSLTFDFVAQTVTITGSQVRLLLPDGGVDVVQAGRVV
                     VDLSQDPPVEVFLAGPKEADARICANLA"
     gene            1776..2420
                     /locus_tag="VFV93_18785"
     CDS             1776..2420
                     /locus_tag="VFV93_18785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5167463.1"
                     /translation="MPWPRTRSAVEAKSIAARPDPPDAATDDAALVAAARANPEAFTL
                     LYERHARQIYRYCNLKLGSREAAEDATSQVFVEALSDLGSFRGSGSGAFGGWLFRIAQ
                     HTVTDMQRRNQRVRGLEPLAESELIDPDHRPEEIVAGRSGLDTIRAAVGTLPTDQRQV
                     IELQLAGLTTQEIARTLGRSANAVRIARFRAFGRLRTIMAQVYPELRGAEGDAP"
     gene            2417..>2701
                     /locus_tag="VFV93_18790"
     CDS             2417..>2701
                     /locus_tag="VFV93_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167464.1"
                     /translation="MIGRTPRQPRLLDRYWSALRFGRGQPSPPPGLDPDLARMAEVLA
                     RESAQLEPGDAFLNDLRQRLASQATSRTGRGWATPALEPRSRSLPASDDAK"
BASE COUNT          483 a          910 c          873 g          435 t
ORIGIN      
        1 cgacgatgac cagaccccgg cgatcgggcc gagtggtccg acaccgacgg ttgaggtaac
       61 cgaaacacct gcggccgtcg tgccggcccc gacggagccg gcgacggcga caaccgaacc
      121 ggccgcgacc gcgacagcga cggctgaacc gagcccgact ccgtcgctgc cgtcgttcgg
      181 gacaattgcg gcgacgatcc cggtcggcgc cggcgtgcga aacatggccg ccggcgaggg
      241 cgcgctctgg gtcaccaacg cgtcggacgg caccgtctca cggatcgatc tggcaacgaa
      301 cgaggtggtc gcgacgatcg aggttgcgcc gccaggggac cagtccgtcg acgcaatcgc
      361 tgtcggcggt ggtgcggttt gggtgacgac ccagggcagc atccagctcg cgcggatcga
      421 cccagcgacc aaccaggtcg tcacgaccat cagcctgaac acggccgtcc atgagctggt
      481 attcgggggc ggtgcgctct ggggcagagg ctcgttctcg aacacgatcg tgcgggttga
      541 tccagcgacc aacaccgtcg tcgcctcgat cgacgtgccg ccaccaaccg cgatcgtcta
      601 cctggacgac gcgctctggg tgatggatgt gagcgatgtc tcgaagattg acccggcaac
      661 gaactcgatc gttgcaaccg ttcactacaa cgacgatcca tccggaccaa ccggaacggg
      721 aatcgtcgca gccggtggca gcctctggct gagcgacgac agccttggcc ccgaactcgt
      781 ccagatcgat ccagcgagcg ccacgatcct cgcgcgagtc gccctgccgg ttgaagtcga
      841 caggatcgcg acggccggta gcgccgatgc cgtctacatc tgcgggtgta ccggcagccc
      901 ggacacgatg atctggcagt tcgatgtcgc gacacatgaa ctgaccgccg cgctcaatgt
      961 ccccgacgcg gaatggctca tctccgatgc agactctctc tggacctaca acggtccgtt
     1021 gcaggaggtc acacgcgtcg acgtgacctc atgacctcgt cttctcaacc atcgacagga
     1081 ggacttccat gactcgtatc ctcaggaggc tgatcctccg cctcgtcgcg ctgcaggcag
     1141 tgctgctcct agtgctgacc ccgacagccc tggcggcgaa tccgacacca ggcctgaccc
     1201 cgcccccgcc atcagatgcg atctgctcga ccagtccaca cgggacgatc tgccacgttg
     1261 atggagcgtt caccagggag gatccggagc tgacggtctg tgacaacgtc gtcgtggatg
     1321 ggtcttctag cggtactgcc agtcgcaagt tgacctacga cgcgacgggc gcgctggtca
     1381 aggagcagcg gcacgttcgc ttcaccggca cactcaccaa tcgctcctcc ggagagtcga
     1441 taggctacgc gggcaccttc agcctgacct ttgacttcgt tgctcagaca gtgacgatta
     1501 ccggcagcca ggtccggtta ctgctgccgg acggtggcgt cgatgtcgtc caggccggtc
     1561 gggtggtcgt tgatctcagc caagacccgc cagtggaggt cttcctggcc ggccccaagg
     1621 aggcagacgc ccggatctgc gccaacctcg cgtaggcagg aggctcctga caagcaccgt
     1681 cccggcgttt gcgtgtcgtc tgaaatactt tcggctcgaa ctgcgccaat agaacagaga
     1741 tctgctgcag gacgccagaa ggagagcgcc gacaggtgcc gtggcctcgt actcgttccg
     1801 cggttgaggc gaagtccatt gcggctcgtc cggatccacc ggacgccgcg acggacgacg
     1861 ccgcgctcgt tgcggccgca cgagccaacc cggaggcgtt cacgttgctc tacgagcggc
     1921 atgccaggca gatctaccgc tactgcaatc tcaagctggg cagccgggaa gcggctgagg
     1981 acgcgaccag tcaggtcttc gtcgaagcac tgtccgacct cggatcattc cgtgggagcg
     2041 gcagcggcgc gttcggcggc tggctcttcc ggattgccca gcacacggtg accgacatgc
     2101 aacgccggaa tcagcgggta cgcgggttgg agccactcgc cgagagcgag ttgatcgacc
     2161 cggatcatcg accggaggag atcgtcgccg gccgcagcgg gctcgacacc atccgtgccg
     2221 ccgtcggcac cttgccgacg gatcaacgac aggtgatcga gttgcagttg gccggtctca
     2281 cgacacagga gatcgcccgg acgcttggac gcagcgcgaa cgctgtccgg atcgcccggt
     2341 tccgagcctt cgggcggctg cgtacgatca tggcgcaggt ctatcccgag ttacgcgggg
     2401 cggaaggaga cgccccatga tcggccgcac accacgtcag cctcggctgc tggatcgcta
     2461 ctggagcgcg ctgcgcttcg gtcgcggcca gccgtcgccg ccgcccgggc tggatcccga
     2521 tctggcccgg atggcggagg tgctggcgcg tgagagcgcg caactcgagc caggcgacgc
     2581 attcctgaac gacctgcgcc agcggctcgc atcacaggcg acctcccgaa ccggccgtgg
     2641 ctgggccaca ccggcactcg aaccccgttc ccgctcgctt ccggcgtctg acgacgctaa
     2701 g
//
LOCUS       DASUGJ010000838         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1109074, whole genome shotgun sequence.
ACCESSION   DASUGJ010000838 DASUGJ010000000
VERSION     DASUGJ010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109074"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..892
                     /gene="purN"
                     /locus_tag="VFV93_18795"
     CDS             248..892
                     /gene="purN"
                     /locus_tag="VFV93_18795"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX5167465.1"
                     /translation="MAGTRMAGRLQVAVFLSGTGRTLENLLALRDVGELEIDIPVVIS
                     SRAGVRGVEIAMAAGIETHVVRRRDYPTPAAMSARIRELLAPHRIDLILLAGFLSQLE
                     ILPEWEGRMLNIHPSLLPLFGGRGLYGSRVHEAVLASGMQVTGCTVHLVNADYDAGPI
                     VLQRSLPILADDTPETLGARVFALECELYPEAIRLFAEGRLMPRDAGRGTQDAG"
     gene            complement(951..1370)
                     /locus_tag="VFV93_18800"
     CDS             complement(951..1370)
                     /locus_tag="VFV93_18800"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX5167466.1"
                     /translation="MDEQRPQDIQPEWQPLEQAVAPVNTPPTPPPPGPKRLYRSSDQK
                     LFLGVCGGLGEYFDIDPTIIRAIFVVMTLLVGTSIAVYAVLALIMPSEASLDADPRTA
                     AQNTINEASDEVRRGIDLAVSRVKEMTGRGTQGTGHE"
     gene            1604..2731
                     /locus_tag="VFV93_18805"
     CDS             1604..2731
                     /locus_tag="VFV93_18805"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5167467.1"
                     /translation="MATDGKQIVRPPVERETIRIGVIGAGFGANVHVPALRRVPGIQV
                     TAICGTNRDRLNDLAAGLEIPASFDDYREMLTSGRVDAVTIATPPHLHHPITLAAIES
                     GLHVLCEKPMARNVAEARDMLRMAREAGVCHAVGHQMRHDPARTQMKKLLDEDFIGRL
                     HSVSVTVYRSSLADPTRRTNDWLIDASRAGGILAAVGSHYIDTLRWWFGEIHWAAGAV
                     STSVDERLVTGRSDMRRVDADDNTAFVIRFASGALGTVHISYTSSAEIGEEIIATGSE
                     GTLAIHDDGRLYGSRHGERIRSLYKPTSTDIHERGKGGIGRHIRSFSILAGEWVWAMR
                     SGTDASPSFEDGAKVQEVVDAVTRSQQLSRWIDLSGNKWPV"
     gene            2811..>3577
                     /locus_tag="VFV93_18810"
     CDS             2811..>3577
                     /locus_tag="VFV93_18810"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5167468.1"
                     /translation="MSYRDELDTPALVVHEEILDRNLTDMAEYAKSCGITLRPHFKTH
                     KTGKVAERQKALGSSGITVAKIGEAEALVDAGVYDDFFIANEIVGPIKHKRLLALMDR
                     AKVRVAVDTLDSARGLDGAMGAAGKTLDVVIELNTGQNRAGVYPNEALGLAETIRNEM
                     PNLRVVGLMTHEGHAGATIAPDGIVSTAIAAGKAITETAEQLREHGFDISVVSVGSTP
                     AAKHTTTIEGITEMRPGTYVFNDNSGFRFGTKPEDCAL"
BASE COUNT          596 a         1172 c         1219 g          590 t
ORIGIN      
        1 aagcttgcca gccagtcgtc gtcaatcgcg ccctgcgaac ggcgttcctg gatgacgacg
       61 ccggtgtcgc ggtggaaggc tctggcggcc ttcgagagga cgcgccgctt gccggccgtg
      121 acgtactggt tgttgacgcc gggtggcagc ggcagcctga gtcggagcgt cttcatcgcg
      181 atggcggcct gcccgcgcgt gtatcatcgg cgctggttcg ggaactgttc tggcgcgggg
      241 agtgatgatg gcgggaacgc ggatggccgg tcgattgcag gtggcggtct ttctgtcggg
      301 aacgggcagg acgctggaga acctgctggc gctgcgggac gtcggggaac tggagatcga
      361 catcccggtc gtcatctcgt cgcgggctgg cgtgcggggc gttgagatcg ccatggcggc
      421 cgggatcgag acgcatgtcg tccgccgccg cgactacccg acgccggcgg caatgtccgc
      481 ccggatacgc gagctgcttg cgccgcatcg gatcgacttg atcctgctgg ccgggttcct
      541 ctcgcaactg gagatcctgc ctgagtggga ggggcggatg ctgaacatcc acccgtcgct
      601 gctgccgctc ttcggcgggc gcgggctgta tggcagccgc gttcacgagg ccgtcctggc
      661 gtcggggatg caggtcaccg gctgcaccgt ccacctcgtc aacgccgact acgacgccgg
      721 cccgatcgtc ctgcaacgca gcctgccgat cctcgctgac gacacgcccg aaacgctcgg
      781 cgcccgcgtc ttcgccctcg aatgcgaact ctacccggag gcgatccgct tgttcgcgga
      841 ggggcggttg atgccgcggg acgcgggacg cgggacgcag gacgcgggtt gattgccgct
      901 tgtcccgtta ttcgtttccc ctgtcccgcg tcctgcgtcc cgcgtactcg ctattcgtgt
      961 cccgtgccct gagttccgcg ccccgtcatc tccttgacgc gcgagacggc caggtcgatc
     1021 cccctgcgaa cctcgtcaga tgcctcgttg atcgtgttct gggctgccgt gcgcgggtcg
     1081 gcgtccagcg acgcctcgga gggcataatc agcgccagga cggcgtagac ggcgatcgac
     1141 gtgccgacca gcagcgtcat gacgacgaag atggcccgga tgatggtcgg atcgatatcg
     1201 aaatactcac caagcccgcc gcagacgccg aggaacaact tctggtcgct gctacggtag
     1261 agccgcttcg ggccaggcgg cggtggcgtc ggcggcgtgt tcaccggcgc gaccgcctgc
     1321 tccaacggct gccactcagg ctggatatcc tgcgggcgtt gctcgtccat gcgtctatcc
     1381 cttccgctgg aacccctctg ccagtggcga tgatacaccg gggcgggaat cgtgacgcgt
     1441 gacgcgtgac gtgagttggg gtatgagggg tgagaggtca cagatcccgc gtcacgcatc
     1501 acgcatcaca cgtcacgaca acaacgttgc cgcaacgtcc ccaaccatgt atacgttctg
     1561 gttggatcgg gaagggaagc ggggcaactg ggtgacggca tcgttggcaa ctgatgggaa
     1621 gcagatcgtt cggccaccgg tcgagcggga gacgatccgg atcggcgtga tcggggccgg
     1681 tttcggcgcg aatgtccacg tcccggcgct acggcgggtg ccgggtatcc aggtcacggc
     1741 catctgcggc accaaccgcg accggctgaa cgacctcgcg gctgggctgg agattccggc
     1801 ctcgttcgac gactaccgcg agatgctgac ctccggccgc gttgatgccg tcacgatcgc
     1861 gaccccgcca cacctgcacc atccgatcac gctggcggcg atcgagtccg gcctgcatgt
     1921 cctctgcgag aagccgatgg cgcgcaacgt cgccgaagcg cgggacatgc tgcggatggc
     1981 ccgtgaggcc ggggtctgcc acgccgtcgg ccaccagatg cggcacgacc cggcccggac
     2041 gcagatgaag aagctgctgg acgaggattt catcggccgg ctgcattcgg tcagcgtcac
     2101 ggtctatcgc tcgtcgctgg cagacccgac ccggcgcacg aacgactggc tgatcgacgc
     2161 gtcacgggcc ggcggcatcc tggcggcggt cggctcccac tacatcgaca cgctgcgctg
     2221 gtggttcggt gagatccact gggcggccgg cgccgtctcg acgtccgtcg acgagcggct
     2281 ggtgacgggc cgcagtgaca tgcgccgcgt cgatgccgac gacaacaccg ccttcgtcat
     2341 ccgcttcgcc tccggcgcgc tcggcaccgt ccacatctcc tacacctcat cggccgagat
     2401 tggcgaggag atcatcgcga ccggcagcga gggcacgctg gcgatccacg acgacggccg
     2461 tctctacggc tcacggcatg gcgagcgcat ccgctcgctc tacaagccga ccagtaccga
     2521 catccacgag cggggcaagg gcggcatcgg ccgccacatc cgctccttct cgatcctggc
     2581 cggcgaatgg gtctgggcca tgcgcagcgg caccgacgcc tcaccctcct tcgaggacgg
     2641 cgccaaggtc caggaagtcg tcgacgccgt cacccgctcg caacagctct cccgctggat
     2701 cgacctcagc ggcaacaagt ggcccgtcta gaacccgtca cgaacatatg gtggtggagt
     2761 aacgtgctac attgcacgca gaatggcagg ctgatcctgg ggagggatag atgagctatc
     2821 gcgatgagct ggacacaccg gcgctggtcg ttcatgagga gattctggac cgtaacctga
     2881 ccgatatggc ggagtacgcc aaatcgtgtg ggatcacgct gcggccccac ttcaagacgc
     2941 acaagaccgg taaggttgcc gagcggcaga aggcgctggg gtcgagcggg atcaccgtcg
     3001 ccaagatcgg cgaggccgag gcgctggtcg atgccggcgt ctacgatgac ttcttcatcg
     3061 ccaacgagat cgtcggcccg atcaagcaca agcggctgct ggcgctgatg gatcgggcga
     3121 aggtgcgcgt cgccgtcgat acgctcgact ccgcgcgcgg gctggacggc gcgatgggcg
     3181 cggccggtaa gacgctggat gtcgtcatcg agctcaacac cggccagaat cgggcgggag
     3241 tctatccgaa cgaggcgctg gggctggccg agacgatccg caacgagatg ccgaacctgc
     3301 gcgtcgtcgg gctgatgacc cacgaagggc acgccggggc gacgattgcg ccggacggca
     3361 tcgtctcaac cgccatcgcg gccggcaagg cgatcaccga gacggccgag cagttgcgcg
     3421 agcatggctt cgacatcagc gtcgtctcgg tcggctcgac accggccgcc aagcacacga
     3481 cgacgatcga gggcatcacc gagatgcgcc ccggcaccta cgtcttcaac gacaatagcg
     3541 gcttccgctt cggcacaaag ccggaggact gcgccct
//
LOCUS       DASUGJ010000839         6054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1666142, whole genome shotgun sequence.
ACCESSION   DASUGJ010000839 DASUGJ010000000
VERSION     DASUGJ010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666142"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1394)
                     /locus_tag="VFV93_18815"
     CDS             complement(42..1394)
                     /locus_tag="VFV93_18815"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX5167469.1"
                     /translation="MGQEATRLDDDQRALGATSGEAAIPDDPDAIEQDIEQTRAEMSG
                     TIDAIQERLNPEALKAQAKEAAHDATIGRVIDAKDQVVEKVQDLTQQATDKVHELTGG
                     NGGDQYGGQSSTSQLTSTARETGGTIVDTIRANPIPAAIAGAGLGWFLTHRSDHQHHQ
                     GRSTYMQNTTMRGGYRGRQMNYRTGYTNWDQRQPDPGHLRAYEGDTLMSQMSSTGGDI
                     VGVIQRHPIPAALAGVGIGWFLMRRSNGGDEGEQPYYYRPAQHTTMRGAYDTRVGYDP
                     RGYGYQSYGSQGNWQQGREDDGGRMSEMAGTAQEAAGDVMHRAQEMGSDAMERVSEYG
                     SMAQEQMSQLPQQAQDQFQHLRYSARQRMQDSPLMMGIVAMGLGLAAGMLLPETEKEN
                     EVFGERRDELMGRAQEMAGQAVERVQDVAQDAAEAAKETVQDAAQQQGQSSQSSATTA
                     "
     gene            complement(1370..1762)
                     /locus_tag="VFV93_18820"
     CDS             complement(1370..1762)
                     /locus_tag="VFV93_18820"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5167470.1"
                     /translation="MQQSRDDRSLGELFSELSRQTSTLIRQEVALAKAEMKQKGSEAG
                     KDIGMMAAGGALAYAGLLALIAMIIIVLANVMPWWLSALIVGSVVVAIGGLMIQRGMS
                     SLKESGVAPEQTIETLKEDKEWAKKQLG"
     gene            complement(1791..2684)
                     /locus_tag="VFV93_18825"
     CDS             complement(1791..2684)
                     /locus_tag="VFV93_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167471.1"
                     /translation="MVSHGAGTSSSSDDQTRGMPGNMGKSDDKAEQVKEAAGQVKEQA
                     SKVTDKAKDQAATRADQQKDRAAEGIHGFADALTQVSDSMRNQNPTVANFADTAASRL
                     ESFASSIQNKDINELVSEVEDLARRQPALFIGGAFLAGVVAARFLKSSSGMSTGSRYD
                     GGYGSYQGRYSQGYGDYDTRGFSSQYSSQYGRPTTGTGYGSTYGSTTSSTYGSTGTTN
                     RSGYGSTYSSTTDTRTTGNRSGMSTGTTGGQDTPGAVRSGTGDVDSRGRGLGTTGSTV
                     SNTDTSSSYDRTRGTSGSSSS"
     gene            complement(2933..3787)
                     /locus_tag="VFV93_18830"
     CDS             complement(2933..3787)
                     /locus_tag="VFV93_18830"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5167472.1"
                     /translation="MPDRTLTTAVLNVASALARISDDALVADWPEPSEPGGFWYGYDD
                     TAREVAFCAYQELRNLASVIAVQRPASTEAQRILAQHQVAYRDLTGALAGVGDDHFDL
                     PPAENEWPLRTVLYHIGLTERGFHALIHWAVSRQGGGGSLPIEMADEYRDEVSDRIDE
                     SGTLDEVLARFDALHLRVLADFSGLDDTDLAAPNIWWEGYEVPVRFRLHRFDTHLREH
                     TIQVDKTLAGIGHSPTEPERLARLLHRALGEVEGALLGAPDVALDRQRSVVAMVDQLT
                     TTLRSLPS"
     gene            complement(3903..4931)
                     /gene="moaA"
                     /locus_tag="VFV93_18835"
     CDS             complement(3903..4931)
                     /gene="moaA"
                     /locus_tag="VFV93_18835"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5167473.1"
                     /translation="MNTRAVDHLTDQFNRPLRDLRISVTDRCNFRCVYCMPKEIFGAG
                     YPFLSREETLSFEEIARLGRIFVNHGVRKFRLTGGEPLVRRNLEELVGMLSEIDGLED
                     IALTTNGSLLSLKKAKALKAAGLRRISISLDSLDNDIFQSMNDVGFPVEKVLQAIDNA
                     LAAGLEPVKIDMVVKRGVNENSIVAMAERFRGTGVILRFIEFMDVGSTNGWRMEDVVP
                     AQEIIETVSAVWPLMPVDPNYPGEVANRWQYLDGQGEIGVIASVTMPFCGDCNRARLS
                     SDGQLYTCLFGIKGHDFRALLRNGASDEEISELLQTVWGNRVDRYSEIRSSNTIHLKK
                     IEMSRIGG"
     gene            5105..6022
                     /locus_tag="VFV93_18840"
     CDS             5105..6022
                     /locus_tag="VFV93_18840"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5167474.1"
                     /translation="MKILVTGGAGFIGSNVVDAYIEAGHQVLVADDLSAGKRENVDPA
                     ATLHEVDIATPAFAELVAAERPDIINHHAAQTSVRHSVGNPLDDCRRNILGSLNVIEA
                     ARQAGVGKLIYISSGGAIYGEPSTLPCPEDHPVVPDSPYGASKHAPEHYLDIYRRVHG
                     LNYTVLRYGNVYGPRQDPYGEAGVIAIFTAQMLAGQQVTINGSGEQERDFVFVGDVVR
                     ANLRALETGDGEAFNIGNGEGTTINQIFGLLRDATGSPHDAAYGPAKAGETFRIYLDI
                     SKAAQGLGWRPEVNLRDGLIQTVASLRTD"
BASE COUNT         1014 a         2009 c         1885 g         1146 t
ORIGIN      
        1 cggagagatc cttcgcggcg gctcaggacg actcggagct gctaggcagt cgttgccgat
       61 gactgcgacg actggccctg ctgctgggcg gcgtcctgga ccgtttcctt ggcggcttcg
      121 gccgcgtcct gggcgacatc ctggacgcgc tcgacggcct gacccgccat ctcctgagcg
      181 cggcccatca gctcatcacg tcgctcgccg aagacttcgt tctccttctc ggtctccggg
      241 agaagcatgc cggccgcgag gccgagaccc atcgcgacga tgcccatcat cagcgggctg
      301 tcctgcatgc gctgccgcgc cgaatagcgc agatgctgga actggtcctg ggcctgctgc
      361 gggagctggc tcatctgttc ctgtgccatc gaaccgtatt cgctgacgcg ctccatcgcg
      421 tccgaaccca tctcctgggc acggtgcatc acgtcgccgg cagcctcctg ggcggtgccg
      481 gccatctcgc tcatccggcc accgtcgtcc tcgcggccct gctgccagtt gccctggctg
      541 ccgtagctct ggtagccgta gccgcggggg tcatagccga cgcgggtgtc gtaggcgccc
      601 ctcatcgtcg tgtgctgggc cggacgatag tagtatggct gctcaccctc gtcgccgccg
      661 tttgacctgc gcatcaggaa ccagccgatg ccaacacccg ccagcgcggc cgggatcgga
      721 tggcgctgga tcacgccaac gatgtcgccg ccggtgctgc tcatctggct catcagcgta
      781 tcgccctcat aggctcgcag gtggccgggg tccggctggc gttgatccca gttcgtgtag
      841 ccggtgcggt agttcatctg gcggccacga tagccgcctc gcatcgtcgt gttctgcatg
      901 taggtactcc gtccttggtg gtgctgatga tccgaacgat gggtcaggaa ccagccaagt
      961 cccgccccgg cgatcgcggc cggtatcgga ttggcgcgga tcgtgtcgac gatcgtgccg
     1021 ccggtctcgc gcgcggtcga cgtcagctgg ctcgtgctgg actgcccacc gtattgatcg
     1081 ccgccgttgc cgccggtcag ttcgtggacc ttgtccgtcg cctgctgggt caaatcctga
     1141 accttctcaa ccacctggtc cttggcgtcg atcacgcggc cgatcgtcgc gtcgtgggcc
     1201 gcttccttgg cctgcgcctt gagcgcttcc ggattcagcc gctcctgaat cgcgtcgatc
     1261 gtcccactca tctcagcgcg cgtctgctcg atatcctgtt cgatggcgtc cggatcgtcg
     1321 gggatcgccg cttctccgga ggtagctccg agcgctcgct gatcgtcatc tagccgagtt
     1381 gcttcttggc ccattctttg tcctctttca acgtctcgat ggtctgctcg ggcgcgacac
     1441 cgctctcctt cagcgaactc ataccgcgct ggatcatcag cccaccgatg gcgacgacga
     1501 cgctgccgac gatcaatgcc gacaaccacc acggcatgac gttggcgagg acgatgatga
     1561 tcatcgcgat cagtgccagc aggccggcgt aagcgagcgc gccgccggcc gccatcatgc
     1621 cgatgtcctt gccggcttcg ctgcccttct gcttcatctc ggctttcgcc agcgcaactt
     1681 cctggcggat caaggtgctc gtctgccgcg aaagttcgga gaacaattcg cccagcgagc
     1741 ggtcatccct gctttgttgc atggtgtacc tctcagtacg ctacagactg ctagctggac
     1801 gacgagccgc tcgtcccacg ggtgcggtca tagctgctgc tcgtgtcggt gttgctgacg
     1861 gtgctgcctg ttgtgccgag tccacgtccg cgtgaatcga catcgccggt cccgctgcgg
     1921 accgcgccgg gcgtgtcctg cccgccagtc gtgccggtcg acatgccgct gcgattgccg
     1981 gtcgttcgcg tatcggtcgt gctgctgtag gtcgagccgt agccgctacg gtttgtcgtg
     2041 ccggtcgagc catacgtcga gctcgtcgtc gatccatagg tcgagccgta gccagtcccg
     2101 gtcgtcggcc tgccatactg cgagctgtac tgggatgaga agccgcgcgt gtcatagtcg
     2161 ccgtagccct gactgtagcg gccctgataa ctgccgtagc cgccgtcata gcggctgccg
     2221 gtgctcatgc cgctcgaact cttgaggaag cgggcggcga cgacgccggc caggaatgcg
     2281 ccaccgatga agagcgccgg ctggcggcgg gcgagatcct cgacctcgct gaccaactcg
     2341 ttgatgtcct tgttctgaat tgaggaggcg aacgactcca gcctgctggc ggcggtgtcg
     2401 gcgaaattag caacggtcgg gttctgatta cgcatcgagt cgctgacttg cgtcagcgca
     2461 tcggcgaacc cgtggattcc ctcagccgca cgatccttct gctgatcggc gcgcgtcgcg
     2521 gcctggtctt tcgccttgtc agtgactttg ctggcctgct ccttgacctg cccggccgcc
     2581 tccttgacct gctccgcctt gtcgtcggac ttgcccatat tgccgggcat gccacgggtc
     2641 tggtcgtcgc tgctggagga tgtacctgca ccgtgagata ccatactgtg gttcctttcg
     2701 atcgatgtcg cgctcgtttc tccacccgtc gcgctcatcc gtcacgacct gtgatgacga
     2761 cggtcatctg gacgggacag atgcaatttg catgcccgac caggccattt gtctatttcg
     2821 acaatggatg atatctaaca catgatgtgt gagttatggc aaacgaaacg cgaattggaa
     2881 tgcgcaaacg cggcgcgaca gccattcgag tgggagagag gtccaacagc ggctagctgg
     2941 gcagcgaccg taatgtcgtc gtcaactgat cgaccattgc gacaactgat cgctggcggt
     3001 caagcgcgac atccggcgcg ccgagcagcg cgccctcgac ttcgcccagc gcgcggtgga
     3061 gcagccgcgc cagccgctcc ggctctgtcg ggctgtggcc gatgccggcc agcgtcttgt
     3121 cgacctgaat ggtgtgctca cgcaggtgcg tatcgaagcg gtgcaggcgg aaccgcaccg
     3181 gaacctcata gccctcccac cagatattcg gggccgccag atcggtgtca tcgagaccgc
     3241 tgaaatccgc gagcacgcgc aagtgcaacg catcgaaacg ggccaggact tcgtcgagtg
     3301 ttcccgattc atcaatccga tcgctgacct cgtcgcggta ctcgtccgcc atctcgatcg
     3361 gcaggctgcc cccgccaccc tggcggctga cggcccagtg aatcagcgcg tggaaacccc
     3421 gctcggtcag cccgatatga tagagaaccg tccgcaacgg ccactcgttc tcggccggcg
     3481 gcaggtcgaa gtgatcgtcc ccaacgcccg ccagcgcccc ggtcaggtcg cggtaggcaa
     3541 cctgatgttg ggcgaggata cgttgcgcct cagtcgaggc gggccgttgg actgcaatca
     3601 ccgacgccag attgcgcagc tcctgatacg cacagaaagc aacctcgcgt gccgtgtcgt
     3661 cgtagccgta ccagaagccg cccggctccg acggctcggg ccagtcagca acgagcgcgt
     3721 cgtcggagat gcgcgccagc gcactggcga cattcagaac agcggttgtc agcgttcgat
     3781 caggcaactc cacggcaact cccatcgcgt gtgtgacagg cgacgaaggt agagccacct
     3841 gcgacggccg tcaagtgcag cgatcgcttg gcccgggctc agtccggacg gatacgggag
     3901 gatcagccgc cgatgcgcga catctcgatc ttcttgaggt ggatcgtgtt ggaggagcgg
     3961 atctccgagt agcggtcgac ccggttgccc cagaccgttt gcaacagctc gctgatctcc
     4021 tcgtcggagg cgccgttgcg cagcagtgcg cggaagtcgt ggcccttgat gccgaacagg
     4081 caggtgtaga gctggccgtc cgacgacagc cgcgcgcggt tgcagtcgcc acagaacggc
     4141 atcgtcactg acgcgatcac gccgatctcg ccctggccgt ccaggtactg ccagcggttg
     4201 gcgacctcgc cggggtagtt cggatcgacc ggcatcagcg gccagacggc gctgaccgtc
     4261 tcgatgatct cctgagccgg gacgacatct tccatgcgcc agccgttggt cgagccgaca
     4321 tccatgaact cgatgaagcg cagaatgacg ccggtgccac ggaagcgttc ggccatcgcg
     4381 acgatgctgt tctcgttgac accgcgcttg acgaccatgt cgatcttgac cggctcaagc
     4441 ccggccgcca gtgcgttgtc gatcgcctgc aagaccttct cgaccgggaa gccgacatcg
     4501 ttcatcgact gaaagatgtc gttgtcgagc gagtcgaggc tgatcgagat ccggcgcaac
     4561 ccggccgctt tcaatgcttt cgccttcttc agcgagagca gcgatccgtt cgtggtcagc
     4621 gcgatatctt cgagaccatc gatctccgac agcatcccga ccagttcttc gaggttgcgg
     4681 cgcacgagcg gctcgccacc cgtcagccgg aacttgcgga caccgtgatt gacgaagatt
     4741 cggcccagcc gggcaatctc ctcgaacgac agcgtttctt cccgggacag gaacggatac
     4801 ccggccccga agatctcctt cggcatgcag tagacgcagc ggaaattaca gcggtcggtg
     4861 accgagatgc gcaggtcgcg aaggggacga ttgaactggt cagtcagatg gtcgacggcc
     4921 cgagtattca tacaggtgag tatacatgcg ttgaagaggt ccggacactg tcccctggcc
     4981 gaatggctga ccaaatcgtg actttcggtt cgtccggatc gaacccggat atccggcata
     5041 atcgagacga cgaggtaccg gtccggcagc gaccgccggc cgaagacaga tagcaggagg
     5101 cacgatgaag attctcgtaa ccggcggggc cgggtttatc ggctcgaacg tggtcgacgc
     5161 ctacattgag gcaggccatc aggtgctggt ggccgacgac ctgtctgccg ggaagcgcga
     5221 gaacgtcgat ccggccgcga cgttgcacga ggtcgacatc gcgacgccgg cgttcgccga
     5281 gctggtcgcc gccgaacgcc ccgacatcat caaccatcac gcggcccaga ccagcgttcg
     5341 ccactcggtt ggcaacccgc tcgacgactg ccgccgcaac atcctcggct cgctgaacgt
     5401 catcgaggcc gcgcgccagg ccggcgtcgg caagctgatc tacatctcca gcggcggcgc
     5461 gatctacggc gagccgtcga cgctgccctg cccggaggat cacccggtcg tgccggactc
     5521 gccatacggc gcgtccaagc acgcgccgga gcactacctc gatatctacc ggcgcgtcca
     5581 cggcctgaac tacaccgtcc tgcgctacgg caacgtctac ggcccccgtc aggacccgta
     5641 cggcgaggct ggtgtcatcg cgatcttcac agcccagatg ctggccggcc agcaggtgac
     5701 gatcaacggt agcggcgagc aggagcgcga cttcgtcttc gtcggcgacg tcgtccgcgc
     5761 caacctccgc gcgctggaga ccggcgacgg cgaagccttc aacatcggca acggagaagg
     5821 gacgacgatc aaccagatct ttggcctact tagggacgca accggctcac cgcacgacgc
     5881 cgcctatggc cccgccaagg ccggcgagac cttccgcatc tacctcgaca tcagtaaggc
     5941 cgcccaaggc ctcggctggc gcccggaagt caacctgcgc gacggcctga tccagacagt
     6001 ggcgtcgttg cggactgact aggaaggcag cgctctccct gtcgtcctga gccg
//
LOCUS       DASUGJ010000840         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1247409, whole genome shotgun sequence.
ACCESSION   DASUGJ010000840 DASUGJ010000000
VERSION     DASUGJ010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247409"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFV93_18845"
     CDS             complement(<1..336)
                     /locus_tag="VFV93_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167475.1"
                     /translation="MTRLTVSLLGPFQVTHETEPVAGFAYDKVRALLAYLVIEADRPW
                     RRETLASLFWPEQDERAARHSLSQALWSLRRALDTVAEDAPLLLVSNTTVQLNPAADL
                     SCDLRRFTEL"
     gene            573..1967
                     /locus_tag="VFV93_18850"
     CDS             573..1967
                     /locus_tag="VFV93_18850"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5167476.1"
                     /translation="MVTATRPTADQVDSLRGQIHGEIIQPGDAGYDTARTIYNAMIDK
                     HPAIVVRCADAADVIAAVGFARAGGYDVAIRGGGHNVSGASMADGGLTIDLSGMNGVR
                     VDPVKRTARVDGGALWRDVDHATHAFGLATPCGIISTTGVAGLTLGGGFGHLSRSLGL
                     SCDNLISADVVTASGELVTASASEHPELFWALRGGGGNFGVVTSFEFQLHPVSTVLGG
                     PIFYDVRHTEHLLRTFRDFMRDVPDEMSAFFSFHQGPPAPFIPEHLHFVPMVAIVVCY
                     NGPIEQGQALLRPLREAAPALVDLAGPIPYPALNSMFDAIYAPGLQHYWKADYLAELS
                     DPMIPIHAKYGPNVPSLQSTMHLYPQSGAIQRVGKDETAYSFRDAEYCHNIVAIDANP
                     ANMPHNMDWMRAYWEELHPYTSGGGYINFMMADETQDRVRATYRGNYDRLARVKRQYD
                     PDNLFHLNQNIRPA"
     gene            2185..2406
                     /locus_tag="VFV93_18855"
     CDS             2185..2406
                     /locus_tag="VFV93_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167477.1"
                     /translation="MIRLAGIVAIASVMAIPGSVAAAQPLVGLTECSHDGCEVVTQRD
                     LNRPTRSVESSTPARITNPTDRTSPHFVP"
     gene            complement(2434..>2943)
                     /locus_tag="VFV93_18860"
     CDS             complement(2434..>2943)
                     /locus_tag="VFV93_18860"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5167478.1"
                     /translation="DMGGADWSDIMAGIDHCIAEGIADPDRLGVGGWSYGGYLTAWGV
                     TQTTRFKAAVAGASITNWISFHGVSTIPGFDASFYAVDPFNWDGHYGQFSPMAHVRNV
                     TTPTLFLHGERDPICPLGQAHEMWRALKELGTDTQLTVYPREPHGIREREHARDVLER
                     ALGWWTRYV"
BASE COUNT          521 a         1049 c          915 g          458 t
ORIGIN      
        1 cagctccgtg aagcggcgca gatcacacga cagatcggcg gccgggttga gctggacagt
       61 cgtgttgctg acgagcagga gcggggcatc ttccgcgacg gtgtccagcg cgcgtcgcag
      121 actccagagc gcctggctca ggctgtggcg ggcggcgcgc tcgtcctgct ccggccagaa
      181 caggctggcc agcgtctcac gtcgccaggg ccggtcggcc tcgatcacca ggtaggccag
      241 cagcgcacgc accttgtcat aggcgaagcc cgcgacaggt tccgtctcat gtgtgacctg
      301 gaacggcccc aggagtgaca ccgtcagccg tgtcatctcc acctcgatcc gtttcgaagg
      361 aacgcttcgt aatcggaaac gcgtccatga tagcgcccgg cggcaaggct aaccaaccgc
      421 cgttgagatg ctgttgatag cccgttgatg ggcgggacgt agggtgtgat gcgtgacgcg
      481 tgacgcgggt tgcgtgacgc gaagggccga accaacccgc gtcacgcgtc acgcatcacg
      541 cgtcacggtc cgaacgtaca aggaggtaga ccatggtcac cgcaacccga ccgaccgccg
      601 atcaggtcga cagcctgcgc ggccagatcc acggcgagat catccagccc ggcgacgccg
      661 gctacgacac ggcccggacg atctacaacg cgatgatcga caagcacccg gcgatcgtcg
      721 tgcgctgcgc cgatgctgcc gacgtcatcg cggccgttgg attcgccagg gccggcggct
      781 acgacgtcgc catccgtggc ggcggacaca acgtctcggg cgcctcgatg gccgatggtg
      841 gcctgacgat cgacctctcc ggcatgaacg gcgtccgcgt cgatcctgtc aagcgaacgg
      901 cgcgagtcga tggcggcgcg ctctggcgcg atgtcgacca cgccacccac gccttcggcc
      961 tggcgacacc ctgcggcatc atctcgacga ccggcgtcgc cgggctgacg ctcggtggcg
     1021 gcttcggcca cctctcccgt agcctgggcc tgtcctgcga caacctgatc tcggccgatg
     1081 tcgtgacggc ctccggtgag ctggtcaccg ccagcgccag cgagcacccc gagctgttct
     1141 gggcgctgcg cggcggcggc ggcaacttcg gcgtcgtcac ctcgttcgag ttccagcttc
     1201 atcccgtcag caccgtcctc ggcggcccga tcttctacga tgtccgccac accgagcacc
     1261 tgctgcgcac cttccgcgac ttcatgcgcg acgtgccgga tgagatgagc gccttcttca
     1321 gcttccacca ggggccaccg gcgccgttca ttcccgagca cctgcacttc gtgccgatgg
     1381 tcgcgatcgt cgtctgctac aacggcccca tcgagcaggg gcaggcgctg cttcggccac
     1441 tgcgcgaggc ggctccggcg ctggtcgatc tggccgggcc gatcccgtat ccggcgctca
     1501 actcgatgtt cgacgcgatc tacgcgccgg gcctccagca ctactggaag gccgactatc
     1561 tcgccgagct gtccgatccg atgatcccga tccacgcgaa gtacgggcca aacgtgccgt
     1621 cactgcaatc gacgatgcac ctctacccgc agagcggcgc gatccagcgc gtcggcaagg
     1681 acgagacagc ctacagcttc cgcgacgcag agtactgcca caacatcgtc gccatcgacg
     1741 ccaacccggc caacatgccg cataacatgg actggatgcg cgcctactgg gaggagctgc
     1801 acccctacac ctccggcggc ggctacatca acttcatgat ggccgacgaa acgcaggacc
     1861 gcgtccgcgc cacctaccgg ggcaactacg accggctcgc gcgcgtcaag cgccagtacg
     1921 atccggacaa tctgttccac ctcaaccaga acatccgccc cgcctgatgg catcgatccg
     1981 ggagcgggaa ccaccccccg ctcccgcgac gatgaccggg taacgctcca tcagcaggcg
     2041 atcggccaca ttcccagcac cgaccggagc cgaccggcat cccaccccgc ctgaacggca
     2101 tcctgaacag catcccgacc gcggctacag gccatcccac cagaaaggca atcgacaatg
     2161 acgtcaatgc acctgcgaca atcaatgatc cgtctggcag gaatcgtggc gatcgccagc
     2221 gtgatggcga tacccggcag cgtcgccgcc gcgcagccgc tcgttgggtt gacggaatgc
     2281 tcccacgacg gctgcgaggt cgtgacgcaa cgcgacctca accgcccaac ccgcagcgtc
     2341 gagtcctcga caccagcccg catcaccaac cccaccgacc gaaccagccc ccacttcgtt
     2401 ccctgagtcc ttggtcgcat gcgatgcccc gaactacacg taccgcgtcc accacccaag
     2461 cgcccgttcg aggacgtcgc gggcgtgttc gcgttcgcgg atgccgtggg gttcgcgggg
     2521 gtagacggtc aactgtgtgt cggtgcccag ttccttgagg gcgcgccaca tctcgtgggc
     2581 ctggccgagc gggcagatcg gatcgcgctc gccgtgcagg aagagggtcg gtgtcgtcac
     2641 attgcggacg tgcgccatcg gcgagaactg accgtagtga ccgtcccagt tgaacggatc
     2701 gacggcgtag aagctggcat cgaagccggg gatcgtgctg acgccgtgga aggagatcca
     2761 gttcgtgatc gacgcgccgg cgacagccgc cttgaagcgc gtcgtctgag tcacgcccca
     2821 ggcggtcagg tagccgccgt agctccagcc gccgaccccg agccggtcgg ggtcggcgat
     2881 cccttcggcg atacagtggt cgatgccggc catgatgtcg ctccagtcgg ccccacccat
     2941 gtc
//
LOCUS       DASUGJ010000841         3923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_692148, whole genome shotgun sequence.
ACCESSION   DASUGJ010000841 DASUGJ010000000
VERSION     DASUGJ010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692148"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1295
                     /locus_tag="VFV93_18865"
     CDS             27..1295
                     /locus_tag="VFV93_18865"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5167479.1"
                     /translation="MAEPTIRIRHQSRFRPVAAGTFHVLLRVTAPPAMRQARTRPPLD
                     VAFVVDRSGSMSGGAFDLARQGVEHALRLLDERDSVSVVVYDDRIDTLLTQRLLERDA
                     RNKAIRRLRRVGPRGSTDLAGGWLTGCDQLAPIADDSRPLRRDGARSIVRTLLLTDGL
                     ANVGMTNHDEIAMHAGELARRGISTSTFGTGAGYDEDLLARMADAGRGHFHHIPNAAA
                     IPEVFAGELGEMLELALRDVTLSLRLPAGWQGSLMNDLPLERHGDWIRLSLGEMASLE
                     SRSLLWELSLPASVDGRIDEIEIQLDGVDAISDLPFVVKAPHPIETSTVPGRPDREVL
                     DELAQMIGARARAEAVRYNKLGQFDRAGEVVLQAARSMPQTETGQAAARELIDVVLPG
                     TGHAASPAQLKQHYARSRNLQRSRKDYSER"
     gene            1299..1736
                     /locus_tag="VFV93_18870"
     CDS             1299..1736
                     /locus_tag="VFV93_18870"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5167480.1"
                     /translation="MATDNGTSIRYTDSLDEIDAGQLDGGFFAGWPNPPSPENHLRML
                     RGSGHVWLAIDTGSGKVVGFVSAISDGVLAAYIPLLEVLRNYQHDGIGGELMHRMLET
                     LSGIYMIDLLCDEELQPWYERLGMRRATGMLVRNYAAQSGSDS"
     gene            complement(1744..2253)
                     /locus_tag="VFV93_18875"
     CDS             complement(1744..2253)
                     /locus_tag="VFV93_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX5167481.1"
                     /translation="MSDLHKQIVIETFATEDDAIRAIQGLKLAGFDTDAITVAGRNHK
                     DARRLAEMVDVSATDEWPDNTRDPRTDLFRIVVKAGDQAEFARRVMIDGLVDATGLPV
                     EFEDESFLDSSVGSGAGVATSTGTATGITPGAGYEGNTGSSVETTPGVLGQFAPGADT
                     PDDEDDFPG"
     gene            2458..>3923
                     /locus_tag="VFV93_18880"
     CDS             2458..>3923
                     /locus_tag="VFV93_18880"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX5167482.1"
                     /translation="MRVALGSIMHETNSFSPIQTPFEAFHEGMPDILDGQRVLDAMRG
                     TKTGIGGFMDVGMEQGWEMIGTVSAHATPSANVQADAHDRLKGRLVQHIREAGKLDGV
                     LLYLHGAMLAENAPDAEGDICKAVRELVGPDVPIITALDLHGNMTEEFCKQVDGVFVY
                     DTNPHIDSWERGVEAAECMAKVLNGEMPRPKVLFVKPPMLPPTINMRTAEGPMTQLLD
                     RGQEWEAGDGIVNVAVFPGFPYADFDQAGTSIIVTATDEEIGRRAVRDMGQFAWSIRD
                     QFLKEIPDVPTAVEQALQQIGEPGGLPVILADVADNPGGGGTGDTTELLRELIRRGAR
                     GAAACVWDPETVQQALQVGIGNEAAFRIAGKAAPEAYGAPLEVRGRVTHLSDGHFTGW
                     GPVMRGRAGNAGPTACIDVNGLKLAVTTFRHAANDRGFFQVAGVQPDREPLLVIKSRG
                     HFRADFQPIARTIIEVDAPGAANPGLSRYEFKNVRRPL"
BASE COUNT          670 a         1231 c         1386 g          636 t
ORIGIN      
        1 cggcgacggt tgaagggagt cccgcggtgg ctgaaccaac gattcgaatc cggcatcaat
       61 cgcgctttcg gccggttgcg gccggcacat tccatgtcct gctgcgggtg acggcgccgc
      121 ctgccatgcg gcaggcgcgg acccggccac cgctggatgt ggcattcgtc gtcgatcgct
      181 ccggatcaat gtccggcggg gcgttcgatc tggcgcgaca gggtgttgaa cacgcgctgc
      241 ggctgctgga cgagcgcgac agcgtctcgg tcgttgtcta cgacgaccgg atcgacacgc
      301 ttctcacgca gcggttgctg gagcgtgacg cgcgcaacaa ggcgattcgg cggctgcggc
      361 gggttgggcc gcgcggctcg accgacctgg ccggtggttg gctgaccggc tgcgatcagc
      421 tcgcgccgat cgcggatgac agccggccgc tgcgccgtga cggcgcccga tcgattgttc
      481 ggacgctgct gctgacggac gggctcgcca atgtcgggat gaccaatcac gacgagatcg
      541 cgatgcacgc cggcgaactg gctcgacgcg gcatcagcac ctcgaccttc ggcaccggtg
      601 ccggctacga cgaagatctg ctggcgcgga tggcggacgc cggccggggg catttccacc
      661 acatcccgaa cgcggccgcg attccggagg tgtttgcagg cgagctcggc gagatgctgg
      721 agcttgcgct acgcgatgtc acgctctcgc tacggctacc ggccggctgg cagggatcgt
      781 tgatgaacga cctgccgctg gagcgacacg gcgactggat ccgcttgtcg ttgggcgaga
      841 tggcgtcgct ggagtcgcgg tcactgctct gggaattgag cctgcccgcg tcggtggacg
      901 ggcggatcga cgagattgag atccaattgg atggcgtcga tgccatcagc gacctgccct
      961 tcgtcgtgaa agccccccac ccgatcgaga cgagcacggt tcctggccgc cccgaccggg
     1021 aggtgctgga cgagctcgcg cagatgatcg gcgcgcgggc gcgggccgag gcggtgcggt
     1081 acaacaagct gggtcagttc gatcgggccg gcgaggtcgt cctccaggcg gcgaggtcga
     1141 tgccacagac tgaaaccggc caggcggcgg cccgcgaact gatcgatgtg gtcctgcccg
     1201 gcactggtca tgcggccagt ccggcccagc tcaagcaaca ctacgcccgt tcgcgcaacc
     1261 tccagcggtc gcgcaaggac tattcggaac ggtagcggat ggcgacggac aacggaacat
     1321 cgattcgata cacggattcg ctcgacgaga tcgacgccgg ccagctcgac ggaggattct
     1381 tcgccggctg gccgaacccg ccatcgccgg agaaccacct gcggatgttg cgcggcagcg
     1441 gccacgtctg gctggcgatt gataccggaa gcggtaaggt cgtcggattc gtcagcgcga
     1501 tcagcgacgg ggtgctggcg gcctacatcc cactgctgga ggtgctgcgc aactaccagc
     1561 atgacgggat cggtggcgag ctgatgcaca ggatgctgga gacgttgtcc ggcatctaca
     1621 tgatcgacct gctctgcgac gaggagctgc agccgtggta cgagcggttg gggatgcgcc
     1681 gcgcgacagg gatgctggtg cgcaactacg cggcgcagtc gggcagcgat tcgtgacgga
     1741 cgatcagccg gggaagtcgt cctcgtcgtc gggcgtgtcg gcgccgggcg cgaactgacc
     1801 gaggacaccg ggcgtcgtct cgacgctgga gccggtgttg ccttcgtagc ctgcgccggg
     1861 cgtgatgccg gtagccgttc cggtgctggt cgcgacgccc gcgcccgagc caacgcttga
     1921 atccagaaag ctctcgtcct cgaactcgac cggcaagcca gtggcatcaa ccagaccgtc
     1981 gatcatcacg cggcgggcaa attcggcctg atcgccggcc ttgacgacga ttctgaacag
     2041 gtcggtcctg ggatcacgcg tgttgtcggg ccactcgtcg gtggcggaga catcgaccat
     2101 ctcggccaga cggcgcgcgt ctttgtgatt gcgccccgca accgtgatcg cgtcggtatc
     2161 gaagccggcc aacttcagcc cctggatggc ccggatcgcg tcgtcctcgg tcgcaaaagt
     2221 ctcaatcacg atctgtttat gcaggtcgct catcgtcgct gtcctccaga tgctgtcgcg
     2281 atatcgatca cgcgacgctc ggctggaaca ggcgcagcct gcatgccaga gcgacgcggt
     2341 gggttgacgg gaaccgcagc gatgatgaga ctggaggccg tcgccgtcaa gcggcgatct
     2401 gcctgagtga ggcgtggagg gccacgcaag cggggaagag gggaaggatc tgcacagatg
     2461 agggtagcgc tcggatcgat catgcacgag acgaattcgt tctcgccaat tcagacgccg
     2521 ttcgaggcgt ttcacgaggg gatgcccgac atcctcgacg gccagcgggt gctggacgcg
     2581 atgcgcggga cgaagaccgg catcggcggc ttcatggacg tcgggatgga gcagggctgg
     2641 gagatgatcg gcaccgtctc ggcccacgcg acgccgtcgg ccaatgttca ggccgatgcg
     2701 catgaccggc ttaaggggcg gctggtccag cacatccgcg aggccggcaa gctggatggc
     2761 gttctgctct acctgcacgg cgcgatgctg gccgagaacg cgccggacgc cgaaggggac
     2821 atctgcaaag cggtccgcga gctcgtcggg ccggacgtgc cgattatcac cgcgctcgac
     2881 ctgcacggga acatgaccga ggagttctgc aagcaggtcg acggcgtctt cgtctacgac
     2941 acgaacccgc acatcgattc ctgggagcgg ggtgtcgagg cggccgagtg catggcgaag
     3001 gtgctgaacg gcgagatgcc ccgtccgaag gtgctgttcg tcaagccacc gatgctgccg
     3061 ccgacgatca acatgcgaac ggctgaggga ccgatgacgc agctgctcga tcgcggacag
     3121 gagtgggaag cgggcgacgg gatcgtcaat gtggccgtct tcccgggctt cccgtatgcc
     3181 gatttcgacc aggccgggac gtcgatcatc gtgacggcga ccgacgagga gatcggtagg
     3241 cgggccgttc gcgacatggg ccagttcgcc tggagcatcc gcgaccagtt cctgaaggag
     3301 attccggacg ttccaaccgc cgtcgagcag gcgctgcaac agatcggcga accgggcggg
     3361 ctgccggtca tcctggccga cgtcgccgac aaccccggcg gcggcggaac cggcgacaca
     3421 accgaactgc tacgggaact gatccggcgt ggtgcgcggg gagcggccgc ctgcgtctgg
     3481 gaccccgaga cggtccaaca agcgttgcag gtggggatcg gcaacgaggc agcgttccgg
     3541 atcgccggca aggccgcgcc ggaagcgtac ggcgcgccgc tggaagttcg cggccgggtg
     3601 acccatctgt cggacggcca cttcaccggc tggggtcctg tcatgcgtgg ccgggctggg
     3661 aacgcgggac caaccgcctg tatagacgtc aacggcctca agctggccgt gaccaccttc
     3721 cgccacgccg ccaacgaccg tggcttcttc caggtggccg gcgtccagcc ggatcgcgag
     3781 ccgctgctgg tcatcaagtc ccgcggccac ttccgcgccg acttccagcc gatcgccagg
     3841 acgatcatcg aggttgacgc tccgggagca gccaatccgg gcttgtctcg ctacgagttc
     3901 aagaacgtcc ggcgtccctt gtg
//
LOCUS       DASUGJ010000842         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1942142, whole genome shotgun sequence.
ACCESSION   DASUGJ010000842 DASUGJ010000000
VERSION     DASUGJ010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942142"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFV93_18885"
     CDS             <1..184
                     /locus_tag="VFV93_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167483.1"
                     /translation="GVLAFLLALAYRTYQTVGSDDLDDMQSTDLLTSPADEVEPDLVA
                     TDRRGDPSPATPAIGN"
     gene            198..1724
                     /locus_tag="VFV93_18890"
     CDS             198..1724
                     /locus_tag="VFV93_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HEX5167484.1"
                     /translation="MNLLLIFPIVIPLGVAALAFLLRQSRTAQRVLGLTGAVLLLVSG
                     IGLLASVWRDGIQAAHIGNWPAPFDITLVADLFSAVMVVLAGATGVAVVVYSLVTIDR
                     PRESFGYYPLLLILLMGVCGAFLTGDLFNLYVWFEVMLIASFVLLALGGERAQMEGAI
                     KYVTLNLIASAFFLVAVGVLYAIAGTLNMADLAVILRGEVGRSGLVTTVAMLFLVAFG
                     IKAAVFPLYFWLPASYHTPPAAVSAIFAGLLTKVGVYALIRVFTLMFVQDVGYTHTLI
                     LYVAALTMISGVLGALAQIDIRRVLSFQVISTIGYMLMGLGILTPLALAGSVFYIIQD
                     VAVKTNLFLISGVIQRLRRTESLPRLGGLYESVPMLSVLFFIPAMSLAGIPPLSGFFA
                     KLALVQAGLSTEHYALVAVALIVSTLTLFSMARVWAAAFWKADPAVKGQGESARSAAR
                     PLLSPIRRWGMFAPIIVLAAITVLVGIAAEPVFTMSRRAADQLLDPTGYIEAVLGVRL
                     "
     gene            1721..2194
                     /locus_tag="VFV93_18895"
     CDS             1721..2194
                     /locus_tag="VFV93_18895"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX5167485.1"
                     /translation="MTGFLWNILLAVVWAAVTGNFSPQNLAFGFAVGFLVMFFGRRVI
                     GSANYGPRLVQALSLAVYFLWQLILSNVRVAYDVLRPNLTLRPGVIAIPLDAYTDAEI
                     VLLANLITVTPGSLSLDVSADRQTLYLHVMHFDDPEEVRRSVKEGYERRILTVTR"
     gene            2245..2481
                     /locus_tag="VFV93_18900"
     CDS             2245..2481
                     /locus_tag="VFV93_18900"
                     /inference="COORDINATES: protein motif:HMM:NF009243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5167486.1"
                     /translation="MLTIALFLAFARLVRGPSLPDRVVALDLITVITVGFIAAYAIDV
                     GQRVFLDAAIVVALIAFLGTVAFARYVERGERNE"
BASE COUNT          386 a          833 c          786 g          596 t
ORIGIN      
        1 cggcgttctt gccttcttac tcgcgctggc ctatcgcacc tatcaaaccg ttggaagcga
       61 tgacctcgac gacatgcagt cgaccgacct gctgacgtct ccggccgatg aagtcgagcc
      121 ggatctcgtc gccaccgacc gtcgcggtga cccgtcgccg gcgacgcccg ccatcgggaa
      181 ctaggggagg agcggtggtg aacctcctgc tgatattccc gatcgtgatc cccctcggag
      241 tcgcggcgct cgccttcctg ctgaggcagt cgcgaacagc ccaacgagtc ctgggactca
      301 ccggagccgt gctcctgctc gtctcgggta tcggtctgct cgcgtccgtc tggcgcgatg
      361 gcatccaggc cgcccacatc ggtaactggc cggcgccgtt cgacatcacc ctggttgccg
      421 atctcttcag cgcagtgatg gtcgtgctcg ccggtgcgac cggcgtcgcc gtggtcgtct
      481 actcgctcgt cacgatcgat cggccccggg agtcattcgg ctactatcca ctcctcctga
      541 tcctgttgat gggcgtctgt ggcgcgttct tgaccggcga tctcttcaac ctctacgtct
      601 ggttcgaggt gatgctgatc gcgtcctttg tgctgctggc gctcggcggc gaacgagccc
      661 agatggaagg cgcgatcaag tacgtgacgc tcaatctgat tgcctcggca ttctttctgg
      721 tcgccgtggg cgtcctctat gcgattgcgg gcaccctgaa catggctgac ctcgccgtca
      781 tactccgcgg tgaggtcgga cgcagcggtc tcgtaacaac cgtcgcgatg ttgtttctgg
      841 ttgcgttcgg catcaaagcg gcggtgtttc ccctctactt ctggctgccg gcttcctacc
      901 acacgccgcc ggcggcagta tcggcgatct tcgccggcct gctcaccaag gttggtgtct
      961 acgcgctcat tcgtgtcttt accctgatgt tcgtgcagga tgtcggctac acccacacgc
     1021 tgatcctcta cgtcgccgcg ctgacgatga tcagcggcgt gctcggcgcg cttgctcaga
     1081 tcgatatccg gcgcgtgctc tcgtttcaag tgatcagcac gatcggctac atgctcatgg
     1141 gcttgggtat cctcacaccg ctggcgttgg ccggctcggt gttctacatc attcaagacg
     1201 ttgccgtgaa gacgaacctc ttcctgatca gcggcgtgat ccagcggctg cgccggacag
     1261 agtcattacc gcgattgggt ggtctctacg agagcgtgcc gatgctgtcc gtgctcttct
     1321 tcatcccggc aatgtcgctt gccggcattc cgccgctctc cggtttcttc gcaaagctgg
     1381 ctctggtgca ggcaggcctg tcgacggagc actatgccct ggtcgcggtg gcgctgatcg
     1441 taagcacgct taccctgttc tcgatggcaa gggtctgggc cgctgcgttc tggaaggcag
     1501 accctgccgt caagggtcaa ggcgagtccg cccggtctgc cgcgcgtccg ctgctctcac
     1561 cgatccgacg ctgggggatg ttcgcgccga tcatcgtcct ggcggcgata acggtcctcg
     1621 tcggtatcgc cgccgaaccc gtgttcacga tgtcgaggcg ggccgccgat caattgttgg
     1681 accccacagg ctatatcgaa gcggtgttgg gagtgcgcct atgaccggat tcctctggaa
     1741 cattctgctg gccgtcgtct gggcggcggt caccggcaat ttttcaccgc agaacctggc
     1801 gttcgggttt gccgttggct tcctggtcat gtttttcgga cgtcgcgtga tcggttccgc
     1861 caactacggg ccgcggttgg tccaggctct gagcctggcc gtctacttcc tctggcagct
     1921 gatcctgtcc aacgtgcggg ttgcatacga tgtgttgcga cccaatctca cactgcggcc
     1981 gggtgtgatc gcgatccccc tcgacgccta caccgatgca gagatcgtgc tgcttgccaa
     2041 cctaatcacg gttacgccag gttcgctcag cctcgatgtt tcggcggatc ggcagaccct
     2101 gtacctgcac gtcatgcact tcgatgaccc ggaagaagtc cggcgaagcg tgaaagaggg
     2161 ctacgagcgg cggatcctga cggtgacgcg atgaactggg atggaatcct caacgtcatt
     2221 caaccgctga acatcgtcta tacgatgctg accatcgccc tgttcctcgc cttcgcccgg
     2281 ctcgtccgcg gccccagcct gcctgaccgc gtggtcgcgc tcgatctgat cactgtgatc
     2341 accgttggat tcatcgccgc gtatgcaatc gacgtgggtc agcgcgtgtt tcttgacgcg
     2401 gcgattgtcg tggcgctgat cgcctttctc ggcaccgtcg ccttcgctcg atacgtggag
     2461 cggggagaac gcaatgaatg agattgtcag cacggccctg ttgctgatcg gcgcgtcgtt
     2521 cctgttgctg gcggcgatcg ggctcctgcg tatgccggac ctattcacgc ggatgtcggc
     2581 cgcaaccaag gggacgaccc t
//
LOCUS       DASUGJ010000843         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1942180, whole genome shotgun sequence.
ACCESSION   DASUGJ010000843 DASUGJ010000000
VERSION     DASUGJ010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942180"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFV93_18905"
     CDS             complement(<1..356)
                     /locus_tag="VFV93_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167487.1"
                     /translation="MANAAVFRQSVDGRIERRRDMEGEQRMRHQGRAIRLLLRGLLIV
                     GVLAAFGASTAAAAPDNKNTFVFHVTCPDAGGALTLTEIDANGNARWVQGSTSVFVVT
                     SVVGEITSEGATFPVN"
     gene            466..>2184
                     /locus_tag="VFV93_18910"
     CDS             466..>2184
                     /locus_tag="VFV93_18910"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5167488.1"
                     /translation="MPMHVGQSFAELLRRARRDAGLTQEELAARAGISTRAVSDLERA
                     INRAPQLETLRLLADALDRPADERQRWTMARRRVASHAHDGGASQPSNGQLPTPLTSF
                     VGRDRELAELVALLGQPDTRLVTITGTGGVGKTRLAIAVGTAVEGALDRFPDGVRFVP
                     LAPLNNPDLVIRAIATALDVEGSSRTSQLTTLIAALRNRRLSLVLDNVEHVAASAPQL
                     ADLLRACPGLTILATSRVPLRVRGEREYKIRPFALPADIAALDLDHLRRLEAIDLFRQ
                     RAQDVATDFQITERNAQVVAGVCRRVDGLPLAIELVAARVRLLPPAALLERLDRRLPL
                     LTGAPHDAPARQQTLRDTIAWSYDLLNPAHQRLFRALGVFQDGWTLDALEAVTGQTEA
                     GSIVLDGLETLIAHSLVRMHEQVNGQPRYSMLETIAEFAREKLDASDEALEARRRHVR
                     HYLRVARQAEPHLKGAEQARWMARLEQEFANLRGALQWLQERAEQGDAAAVVTSFQLA
                     DPIWWFWHIHGHMREGRQQFDTIMRLLQEQQVVVTALTGPEEWSRMWARTLFYVAALD
                     TWQADYH"
BASE COUNT          409 a          711 c          699 g          365 t
ORIGIN      
        1 aagttgaccg ggaacgttgc cccctcactc gtgatctcgc cgacaaccga ggtcacgacg
       61 aacacacttg ttgaaccttg aacccagcgg gcgttcccat tagcatcgat ctccgtcaag
      121 gtgagtgcgc caccagcgtc aggacaggtg acatggaaca cgaacgtgtt cttgttgtct
      181 ggcgcggcgg cagctgtact ggcgccgaac gccgccagga caccaacgat cagcagtccc
      241 cggagcaaca ggcggatggc gcgtccttga tgtctcatac gttgttctcc ttccatatcg
      301 cgccgtcgct caattcgtcc atcgacagat tggcggaaca ccgccgcatt cgccatgacg
      361 aaaccacaga atcactcacc gaattctctg ttcgggcggt ccgatttgca tcttgccgga
      421 cgtgctatgc tgggtggaca aggtcaccaa caggtaggtc cactcatgcc tatgcatgtc
      481 gggcaatcgt tcgccgagtt gctgcgtcgc gctcgacggg acgcaggact gacccaggaa
      541 gagctagccg cccgggcggg catcagcacg cgcgcggtca gcgacctgga gcgcgcgatc
      601 aatcgtgccc cgcaactgga gacgctgcgg ctgctagccg acgcgctcga ccgtccggca
      661 gacgagcgtc aacggtggac gatggcgcga cgccgagtcg ccagccacgc tcacgacggc
      721 ggggcgtcac aaccatccaa cgggcagctt ccaacaccgc tgacctcgtt cgtcggtcga
      781 gatcgagagt tggccgagct tgtcgccctg ctggggcaac cagacacacg gctcgtgacg
      841 attacgggaa cgggtggcgt cgggaagacg cggctggcga tcgctgtcgg cacggccgtc
      901 gaaggagcgc tggatcgatt ccccgacggc gtccggtttg tgccgctcgc cccgctcaac
      961 aacccggacc ttgtcatccg cgcgatcgcg acagcgcttg atgtcgaagg ctccagccgc
     1021 acatcgcagc tgacgacact catcgccgcc ttgcgaaaca ggcgcctgtc gctcgtcctg
     1081 gacaacgtcg agcatgtcgc tgccagcgcg ccacagctcg cggacctctt acgggcctgt
     1141 ccggggctga cgatcctggc aacgagccgg gtgccgctgc gcgtgcgggg tgagcgggag
     1201 tacaagatca gaccattcgc gctgccggcc gacatcgccg cgttggatct cgatcacctg
     1261 cgacgattgg aggcgatcga cctgttccgg cagcgggcac aagacgttgc caccgatttc
     1321 cagatcacag aacggaacgc tcaggtcgtc gccggcgtct gcaggcgagt agatggcttg
     1381 ccgctggcga tcgagctggt ggcggctcgt gtccggctgc tcccaccggc ggcgctcctc
     1441 gaacgcctcg accggcggct gccgctgctg accggcgcgc cgcacgacgc cccggcccgg
     1501 caacagacgc tccgcgacac gatcgcctgg agctatgacc tgctcaatcc ggcacatcag
     1561 cgcctgtttc gcgcgctcgg cgtcttccag gacggctgga cactcgacgc gctcgaagcc
     1621 gtcacgggac agaccgaggc aggctcaatc gtgctcgatg gactggaaac gctgatcgct
     1681 cacagtctcg tacgcatgca cgaacaggtg aatggccagc cgcgctactc gatgctggag
     1741 acgatcgccg agttcgcgcg cgagaagctc gacgcgagcg acgaggcgct ggaggcgcgc
     1801 cggcgacatg tccgtcacta cctgcgggtc gcgcggcagg ccgagccaca cctgaaaggc
     1861 gccgagcagg cgcgctggat ggcgaggctg gaacaggagt tcgccaacct gcggggtgcg
     1921 cttcagtggc tgcaggaacg agcggagcag ggtgatgcgg cagcggtcgt taccagcttt
     1981 cagcttgccg atcccatctg gtggttctgg cacattcatg gacacatgcg tgaaggacgc
     2041 caacagttcg acaccatcat gagactgctt caagaacaac aggtggtggt cacggcatta
     2101 actggcccgg aagagtggtc gcgcatgtgg gccaggacgc tcttctatgt agccgcgctt
     2161 gacacctggc aggcagacta ccac
//
LOCUS       DASUGJ010000844         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1942264, whole genome shotgun sequence.
ACCESSION   DASUGJ010000844 DASUGJ010000000
VERSION     DASUGJ010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942264"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="VFV93_18915"
     CDS             complement(<1..1385)
                     /locus_tag="VFV93_18915"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5167489.1"
                     /translation="MRQSPAQVVVECLRRNGVETIFGLDGDHVIYLYDALSDARDIDV
                     VTVMHENNASIAAELYARVSGRPGVVMVTAGPGATNSLSGIAGAYAAGVPIVHISGGV
                     PAGAAQEAFHGVDDPYVLQKVFEPVTKLSLRIEDAAEIPATIDRAFVVATQGRPGPVH
                     VEIAVSALTSGEMELPDAVERPATQPPDTNIAVIAERIDASAKIALVAGKNAFWPEVS
                     EALVSLAEHLQAPVVHVWDSHGAMPTVHPLALGLWRAGNSNEFVDATFDSADLVLGVG
                     VRRGNESGVTLAKQLGDRFVIIDAVDEPSDNGHVETASIPALATALRAIARETRERPA
                     DASVLDHCAIAQRLFKAGMTVEIDRYRDANPWPISVALESLAEKMTPETIVVSDVSNV
                     KIWAPVQLPTYNSLSHLQSGSWGAMGYVVPGVLGAALARPDRKIVGVVGDAAFLMGSN
                     DFATICALGLPV"
     gene            1542..1928
                     /locus_tag="VFV93_18920"
     CDS             1542..1928
                     /locus_tag="VFV93_18920"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5167490.1"
                     /translation="MGSWVKGIPAITLFAEDLETTKQFYRDVFDVPLVFEDDNSAAFN
                     FGNAIINLLKASEAPELIGPADVASPDTGSRVQFTISVEDVDATCAELASRGVELLNG
                     PIDRPWGQRTACFSDPAGHVWEITRW"
     gene            complement(1971..>2886)
                     /locus_tag="VFV93_18925"
     CDS             complement(1971..>2886)
                     /locus_tag="VFV93_18925"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5167491.1"
                     /translation="VGVILPIGEDLDTGEPLPYSAVRALALQAEAAGFDSVWVPDHLL
                     FRLPEPEPFGIWESWSILAGLAEATERVELGPLVMCVPFRNPALLAKMAITIDEISGG
                     RFILGLGAGWHQPEFEAFGLPFSHLADQFEEGLKIICPLLREGRVDFSGDYASAPDCE
                     MRPRGPRPGGPPILIASRGPRMMRLAAQYADQWNAAWSGRPALFHPRHAALVEACHAV
                     GRDPATIAVTVGLYILYPEHLSQQEAETMPESYYDPDKALRGTPDEIAAGLREYIDLN
                     VDHLICEMIPNTAETLAEFAKALRLLRG"
BASE COUNT          472 a          950 c          990 g          474 t
ORIGIN      
        1 actggcagtc ccagcgcgca gatcgtcgcg aagtcgttcg agcccatcag gaaggccgcg
       61 tcaccgacga caccgacgat cttgcggtcc ggccgggcca atgccgcacc gaggacaccg
      121 gggacgacgt agcccatcgc gccccaactg cccgattgca ggtgcgagag cgagttgtag
      181 gttgggagct ggaccggcgc ccagatcttg acgttgctga catcgctgac gacgatcgtc
      241 tccggcgtca tcttctccgc cagactctcc agcgcgacgc tgattggcca ggggttggcg
      301 tcccgatagc gatcgatctc gaccgtcatc ccggccttga acaatcgctg ggcgatggcg
      361 cagtggtcga ggacggaggc gtcggccggc cgctcgcggg tctcgcgggc gatggcgcgg
      421 agcgccgtcg ccagcgccgg gatcgaggcg gtttcgacgt ggccgttgtc ggacggctcg
      481 tcaacggcgt cgatgatgac gaaccgatcg cccagctgtt tggccagcgt gacaccggac
      541 tcgttgccgc gccggacgcc aacgcccaac accaggtcgg cgctgtcgaa ggtcgcgtcg
      601 acgaactcgt tgctgttacc ggcccgccag agaccgagcg cgagcgggtg gacagtcggc
      661 atcgcgccgt gcgagtccca gacatggacg accggcgcct gcaggtgctc ggcgaggctg
      721 accaacgcct cgctgacttc tggccagaac gcgttcttgc cggcaaccag cgcgatcttt
      781 gcgctcgcgt cgattcgctc ggcgatgacg gcgatgttcg tgtcgggcgg ctgcgtggca
      841 ggccgctcga cggcgtcggg cagctccatc tcgcccgagg tcagcgcgct gacggcgatc
      901 tcgacgtgga ccgggccggg ccgtccctgg gtcgcgacga cgaaggcgcg gtcgatggtg
      961 gccgggatct cggcggcgtc ctcgatccgc aatgagagct tcgtgacggg ttcgaagacc
     1021 ttctgcagca cgtatggatc gtcaacaccg tggaacgcct cctgggctgc gccggccggg
     1081 acgccgccgg agatgtggac gatcgggaca ccggccgcgt acgccccggc gatgccagag
     1141 aggctgttgg tcgcgcccgg cccggctgtg accatcacga cgcccggccg gcccgagacg
     1201 cgcgcgtaca gctcggccgc gatcgaggcg ttgttctcgt gcatgacggt cacgacgtcg
     1261 atgtcgcgtg cgtcggagag cgcgtcatac agatagatga catggtcccc gtcgagccca
     1321 aagatcgtct cgaccccgtt ccggcgaaga cattcgacga ccacctgtgc cggtgactgg
     1381 cgcatgtgct gacgcccctt cccctcgtga accgatgcct cactcctgaa cgcgcccagt
     1441 atatgcgcag tacgatcgtg tcgaactcgg ctgtgaccga acgacaatct acgggatgat
     1501 gtgcatcccc tggttgatgc tgcaccggaa agggtggaaa cgtgggttcg tgggtgaagg
     1561 gaatcccggc aatcacgttg tttgccgagg atctggagac gacgaagcag ttctatcgcg
     1621 acgtcttcga cgtgccgctg gtgttcgagg atgacaattc ggccgcgttc aactttggca
     1681 acgcgatcat caacctgctg aaggcgagcg aggcgccgga gctgatcggg ccggcagatg
     1741 tcgcgtcgcc ggataccggg tcacgggtgc agttcacgat cagtgtcgag gatgtcgacg
     1801 cgacctgcgc ggagctggcg tcgcggggcg tcgagctgct gaacggcccg atcgaccggc
     1861 cctggggcca gcggaccgcc tgcttcagcg acccggccgg tcatgtctgg gagatcacca
     1921 ggtggtgaac cggccgtgcg agtactgccg cgctcgcgtc gagcggtacg ctagccgcgc
     1981 agcagccgca gcgccttcgc gaactcggcc agcgtctcgg ccgtgttcgg gatcatctcg
     2041 cagatcaggt gatcgacgtt caggtcgatg tactcccgca gtccggccgc gatctcgtcc
     2101 ggggtgccac gcagggcctt gtccgggtcg tagtacgact ccggcatcgt ttcggcctct
     2161 tgttgggaga ggtgctcggg atacaggatg tagagcccga ccgtgaccgc gatcgtcgcc
     2221 ggatcgcggc cgaccgcgtg gcaggcctcg acaagggcgg catgtcgcgg gtgaaagagt
     2281 gccggcctgc ctgaccaggc ggcgttccac tggtcggcgt actgcgccgc cagccgcatc
     2341 atgcgtggtc cgcgcgacgc gatcaggatc ggcggcccgc cgggacgtgg accgcgcggc
     2401 cgcatctcac aatccggcgc gctggcgtag tcgccgctga aatcgactcg gccctcgcgt
     2461 agcagcgggc agataatctt cagtccctcc tcgaactgat cggcgaggtg gctgaacgga
     2521 aggccgaacg cctcgaactc cggttggtgc cagccggcgc cgaggccgag gatgaagcgc
     2581 ccgcccgaga tttcgtcgat cgtgatcgcc atcttggcta gcagagccgg gttgcggaac
     2641 ggcacgcaca tcaccagcgg gccaagctcc acccgctcgg tcgcctcggc gagtccggcc
     2701 aggatcgacc agctctccca gatcccgaac ggctcgggct ccggcaggcg gaacagcagg
     2761 tggtccggta cccagaccga gtcgaacccg gccgcctccg cctgcaacgc cagcgcccgc
     2821 accgccgaat acggcagcgg ctcgcctgta tcgagatctt cgccgatcgg cagaatcacc
     2881 ccaact
//
LOCUS       DASUGJ010000845         4152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1248305, whole genome shotgun sequence.
ACCESSION   DASUGJ010000845 DASUGJ010000000
VERSION     DASUGJ010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248305"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFV93_18930"
     CDS             complement(<1..1002)
                     /locus_tag="VFV93_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167492.1"
                     /translation="MLRDSSGTRCHLRRFRRPGGLDSIGSRAWFAGPIVVVALALAAA
                     GWLAVRNTPPVVSWLDVGSPGDAAHITGAFSPERNQTETYRWTTSSAVVTVPAPVGAR
                     YRIDLAIASIGDQQRTLTLTCPAGETTAQLATGQTPPVVTIECDSDGEIPIQISVPEI
                     RVDGDGRPLGVALRDVRVTSLDSNLTAVWRLRAGLVAAAAIVGLWLAVGALAGTRIAS
                     AATATSALVAVAISTRHPEWTLSMIAGPGGLVALSAGYAAWLLQQGRRTGPLVLGIVS
                     IVAWLLVWPPNPSILLPALWTPHDHAGYVLLPWLAALAIIAAALVDGPRATTMAAAGA
                     "
     gene            1034..1549
                     /locus_tag="VFV93_18935"
     CDS             1034..1549
                     /locus_tag="VFV93_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167493.1"
                     /translation="MAVVGVLLVGTLVALPAGGQAPTSPLAPINEVRDREELPPIASS
                     DALDALAASYLSEMLVSRCLCPTLDGDRGAERLLAEVRPALDNDAVIVEAGLIAGYDT
                     TAERAIRTTALDPANASAVAGQWMALAGIASAVVEAGTNWLAPPPGGIGPEIELGGYT
                     VVVIVLAGSAN"
     gene            1546..2610
                     /locus_tag="VFV93_18940"
     CDS             1546..2610
                     /locus_tag="VFV93_18940"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX5167494.1"
                     /translation="MSRLRVLLDATWAAGRGGTARYVRSLADALSADPTIDIIVVRSP
                     RLTRGPRAVRLPLNGSVHLLWTQLCLPLLARHHGADVVQSTMTAPLRCPRPVVLTLHD
                     ALDFVPELRPSRIWSWYMQTVGVLAARRAAVVLTGTRASAAEIVRCYRLAPEKARVTP
                     YGSAMVRLTVDCHDTTGANPCPGCTYALLVGTADKRKDFETALRAIALVRATGSDLAA
                     VVVGSAPAAYADAAWVRVVVDIDDGQLASLYRRAALVLVSSRHEGYGLPVLEALAFGT
                     PVVASDIAALREVGGEAVRYAAPGDAEAFARQIVPLLEDPAGERERVRRSTSAGSVAR
                     WGATARATTDVYREVLGART"
     gene            2616..3770
                     /locus_tag="VFV93_18945"
     CDS             2616..3770
                     /locus_tag="VFV93_18945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5167495.1"
                     /translation="MNGKTILFVHPSDELYGADRCLLELVRGLPPTTRLIAVLPRDLE
                     YTGELSRELTAAGVAVRDLDFAVLRRDGLRLRYWPALARRLLAGTWELAKLARDERAD
                     VVHTNTLAAVSGPLAACMARRAHVWHVHEDIRDEAWPIKLAFRMLALLPGRVIANSRA
                     TATALAGPFGVLHRKTTVVYPGIDTRGRPEPKAASEHAGDALRVAFVGRLSPRKGVTE
                     LLEAVAHVRDQGIALQLEIFGTAPPGQDWREGQYRRRAEGLGIGDVVRFAGFVADVRR
                     KLTSVDVLVVPSQRPEPFGRVILEGMAAGCAVVACRTGGGSDEIIEDGVAGLYCGSEP
                     EEIANAIGRLARDFGLRARLGAQATASVQARFSLDAYCNGIMRVYREMLR"
     gene            3868..>4152
                     /locus_tag="VFV93_18950"
     CDS             3868..>4152
                     /locus_tag="VFV93_18950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5167496.1"
                     /translation="MSNAGSPLRIALLGTRGIPANYGGFETCAEELATRLAERGHRVT
                     VYCRKPQIQFPGSHYRGVRLIKLPTIRNKYLDTIVHSTIASVDAIVRRYDV"
BASE COUNT          658 a         1237 c         1530 g          727 t
ORIGIN      
        1 cgcaccggcg gcagccatcg tcgtggcgcg tggcccgtcc acgagggcgg ctgcgatgat
       61 ggccagcgcg gcgagccagg gcagcagtac gtagccggca tggtcgtgcg gggtccagag
      121 cgccggtagc aatatcgacg ggtttggcgg ccagacgagg agccaggcga caatggagac
      181 gatgccgagt accagcggtc ctgttcttcg gccctgctgt aggagccagg cagcgtatcc
      241 tgcggagagc gccacgagac cgcccggccc ggcgatcatc gacagggtcc actccggatg
      301 gcgggtgctg atcgcgacag cgacaagcgc cgaggtagcg gttgcggccg atgcgattcg
      361 cgttccagca agggcgccaa cagccagcca caatccgacg atggcagcgg cggcgacaag
      421 gccggcacga aggcgccaga ccgcagtcag gttgctgtcg aggctggtga cacgaacatc
      481 gcgcagggcg acgccgagcg ggcgtccgtc gccgtcgaca cggatttcgg gtacgctgat
      541 ctgaatcgga atctcgccgt ccgagtcgca ttcgattgtg acgaccggcg gagtctggcc
      601 ggtcgcgagt tgcgccgtcg tttcgccggc cggacaggtg agtgtcagtg tgcgttgctg
      661 atcgccgatc gaggcgatgg cgagatcgat ccggtagcga gcgccaaccg gcgctgggac
      721 ggtgacgaca gctgatgagg ttgtccagcg ataggtctcg gtctgattgc gttcgggtga
      781 gaaggcgcca gtgatgtgtg cagcgtcgcc gggtgagcca acgtccagcc aactgacgac
      841 gggcggcgtg ttgcgaacag ccagccaacc ggccgccgcg agcgcgagcg cgacgacgac
      901 gatcggcccg gcgaaccagg ctcgtgagcc gatcgaatcc agtcctcccg ggcggcgaaa
      961 tcgtcgcagg tggcacctcg tcccgctgct gtcgcgtaac acgcgtcgtc tgagtcgggc
     1021 ggattgtatc cggatggcgg tggtcggcgt gctgctggtg ggtacgctgg ttgcgctccc
     1081 ggccggcggg caggcgccga catccccgct ggcgccgatc aacgaggttc gcgatcggga
     1141 ggaattgcca ccgattgcat caagcgacgc cctggatgca ctcgcggcga gctacctgag
     1201 cgagatgctc gtttcacggt gtctgtgtcc aactctggac ggggatcggg gggccgagcg
     1261 gttgctggca gaggtccggc cggcgctcga caacgatgcc gtcatcgtcg aggcggggtt
     1321 gatcgccggc tacgacacca cagccgagcg cgcgatccgg acgacggctt tggatccggc
     1381 aaacgccagc gcggttgccg gtcagtggat ggcgctggcg gggatcgcgt cggcggtggt
     1441 ggaggcaggc acgaattggc tggctccgcc gcccggtgga atcggacctg agatcgagct
     1501 tggcggctac acggtcgtcg tgatcgtcct ggcaggatcg gcgaattgag cagactacgg
     1561 gtgttgctcg acgcgacctg ggcggccggt cgaggtggaa ccgcgcgcta cgttcgttcg
     1621 ctggcggacg cgctttcggc ggacccgacg atcgacatca tcgtcgtgcg ttcgccgcgc
     1681 ctgacgcgag gtccgcgcgc agtgcggcta ccgctgaacg gcagcgtgca cctgctctgg
     1741 acgcaactct gcttgccgct gctggcacga caccacggcg ctgatgtcgt ccagtcgacg
     1801 atgacggccc cgctgcgctg cccgcgacca gtcgtgctga cactccacga cgcgctcgat
     1861 ttcgtgccgg agctacgacc gtcgcggatc tggagctggt acatgcagac ggtcggagtg
     1921 ctggccgccc gccgcgcggc agttgtgctg accggaacga gggccagcgc cgccgaaatc
     1981 gtgcgctgtt accggctggc acctgaaaag gcgcgagtaa cgccgtacgg ttcggcgatg
     2041 gtcagactca ccgtcgattg tcacgacacg acgggcgcga atccctgtcc tggatgcacg
     2101 tacgcgctgc tggtcgggac cgcggacaag cggaaagatt tcgagacggc gctgcgagca
     2161 atcgcgctgg tacgtgcgac cgggagcgat ctggcggcag ttgtggttgg cagcgccccg
     2221 gcggcttatg ccgatgccgc gtgggtacgc gttgtggtgg acatcgacga tgggcaattg
     2281 gcatcgttgt atcgacgggc cgcgttggtg cttgtgtctt cgcgtcacga ggggtacggg
     2341 ctgccggtgc ttgaggcgct cgcgttcggg acgccggtcg tggcctcgga tattgcagcg
     2401 ctgcgcgaag ttggcggcga ggctgtccgc tacgccgctc ccggcgacgc agaggccttc
     2461 gctcggcaga tcgtgccgtt gttggaggat ccggccggcg agcgagagcg cgttcgccga
     2521 tcgacgtccg ctggttcggt cgccaggtgg ggagcgacgg cgcgagccac aaccgatgtc
     2581 tatcgagaag tcctcggtgc gcgcacgtga ccgggttgaa tgggaaaacg attctcttcg
     2641 ttcatccaag cgacgagctg tacggcgccg accgctgtct gctggaactg gtacgtggcc
     2701 tgcctccgac gacgcggctg atcgctgtcc ttcctcgcga tcttgaatat accggagaac
     2761 tatcgcgcga gttgacggca gccggtgtcg ctgttcgcga tctggatttc gctgtccttc
     2821 gacgcgatgg gctacgcctg aggtactggc cggcgctggc tcggcggctg cttgcgggaa
     2881 cgtgggaact cgcgaagctg gcgcgagacg aacgagcaga cgtcgtccat accaatacgc
     2941 tggcggccgt ctccgggccg ttggcagcgt gtatggcacg gcgcgcgcac gtctggcacg
     3001 tgcacgagga cattcgggac gaagcgtggc cgatcaagct ggccttcagg atgctggcac
     3061 tcctgccggg acgggtgatc gcgaactcgc gggcgacggc gacggcgctg gcaggaccat
     3121 tcggtgtcct tcatcgcaag acgactgtcg tctatcccgg tatcgacaca cgtggacgac
     3181 ccgaaccgaa ggcagcgagc gaacacgctg gcgatgctct gcgggtagcg ttcgtcggtc
     3241 gcctgtcgcc tcgaaaaggg gtcaccgagc tgctcgaggc ggtcgcccac gttcgtgacc
     3301 agggtattgc gcttcaactg gagatctttg gaactgcgcc gcctggccag gactggcgcg
     3361 aggggcagta tcggcggcgg gctgaggggc tggggatcgg tgatgtcgtg cggttcgccg
     3421 ggttcgttgc ggacgttcgc cggaagctga cgagcgtcga tgtgctggtg gtgccgtcgc
     3481 agcggccgga accgttcggt cgggtcatcc tggaggggat ggcagccggc tgcgccgttg
     3541 tcgcttgccg gactggcggt gggagcgatg agatcatcga ggatggtgtg gccgggctgt
     3601 attgcggcag cgagccggag gagatcgcga acgcgatcgg gcggctggca cgcgactttg
     3661 ggctgcgtgc acggctcggg gcgcaggcga cggcgagcgt tcaagcgcgg ttctcactcg
     3721 acgcctactg caatgggatc atgcgcgtgt atcgtgagat gctacgctga tcgttgccgg
     3781 ccaaccggca gtgcggcaca atccgcgttg ctggtcctgt ccgtagcgaa tgacgtgtct
     3841 gccggtcgcg ggttgggagt cgcatccgtg tcgaatgctg ggtcgccgct gcgcatcgcg
     3901 ctgctgggaa cgcggggcat tccggccaac tacgggggat tcgagacctg tgccgaggag
     3961 ctggcgacca ggttagcgga gcgcgggcat cgggtgacgg tctactgccg caaaccacag
     4021 atccagtttc caggatcgca ctaccggggc gttcggctca tcaagctgcc gacgatccgc
     4081 aacaagtatc tcgacacgat cgtccattcg acgattgcgt cggtcgatgc gattgtccgg
     4141 cgctacgacg tg
//
LOCUS       DASUGJ010000846         4347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_136059, whole genome shotgun sequence.
ACCESSION   DASUGJ010000846 DASUGJ010000000
VERSION     DASUGJ010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136059"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFV93_18955"
     CDS             <1..207
                     /locus_tag="VFV93_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167497.1"
                     /translation="AREESDRQRRLLDLAWVKPSCRCCRLHCWSPSGLLFSGDDRFVN
                     HTHSMREEQAGHIPHDNESRLETG"
     gene            complement(268..2589)
                     /locus_tag="VFV93_18960"
     CDS             complement(268..2589)
                     /locus_tag="VFV93_18960"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5167498.1"
                     /translation="MPDAARPDEVTTLPRIRTRLIGRERELATLTGLLQQEDVALVTL
                     IGPGGVGKTQLALAVAAALNDRFPDAIRFVQLAPLNDAALVVPAIASTLGISEQPGQI
                     LLNQVISAIGNRRLLLVLDNVEHVIEAATAVGELIAACRELIVLATSRTPLRLHDERE
                     YPLRPLDLPASDSPSSCVDLAAYGATALFVERAARVNPEIATTNDTPAIITEICRRLD
                     GLPLAIELAAARTKMLSPPALLDRLEQRLPLLSGGPRDLPARQRTMRDTIAWSYDLLA
                     SDEQRMFRALSAFLGGWTLEAAEAVCGQEELFERLAALVDHSLIRQIEQPDGAIRFGV
                     LETIREFALDQLQAHGEQEEIRERHARYFLALAIEAGQHLMRAGGMEWLRRLDADYSN
                     LRLALDWALDHDVETAVRAAAALWNFWLTHAHFTDGRQVLEAALAADVIVSDPVRASG
                     LDTAASLASWQGDFKRADELFEQSLTIFRTLGDDWNIANTLRGMCRSAMAEGDFERAA
                     RIGEESLAIFRTLNDPIGIQGAVGNLGWNALGTGDLERGRAMLEEALALARDLQSTPG
                     IANYTAGLAYNALDTGNLDGATSLFTETVRLCLEIDDTRFIALSFEGFGRIAARRGDL
                     ERAARLFGAADGLRTAIGMPVVEAFLGEMLAHDIASVQSRLEADAYQAAWHAGQAMPV
                     ADAIAFALDSARPTNVSNDSASSTQTASLGVSARELDVLRLLVNGASNQEIAEALYIS
                     PHTVATHVANIMNKLGVESRTAAATYAIRHGLV"
     gene            2955..3494
                     /locus_tag="VFV93_18965"
     CDS             2955..3494
                     /locus_tag="VFV93_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167499.1"
                     /translation="MCCLVAILAGVAWGIGWQSGSAASPKLAIDAPEEALAGEPIEIR
                     LVIDDANGIAAFDATIGFDTSIASLQSFDQRANDLVDLGRDVKALGPVELPDGVAVGA
                     YACPAESCADLAGKSRNTAQGTGSFRLAAFTIVGWQPGPLTIDLTTAQFTDGSGRPVE
                     VDLSGATVTVEVMPNGEVQ"
     gene            3496..>4347
                     /locus_tag="VFV93_18970"
     CDS             3496..>4347
                     /locus_tag="VFV93_18970"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HEX5167500.1"
                     /translation="MTHLFSRTTRLLLAALTVVALLLSLLPAGQLAAQPANVDPSLAY
                     LDLDSDGQITEIDLMSVTTAWESRKLSGDACSGPAAADVTGDGCVTIADVQTLAANLG
                     VSVLGPSAMAERSRAMVSSDPTFVVTTTSDAPDSNPGDGVCQIAGGGCSLRAAIDESN
                     RMQGANTINFNIPGSGVHVINVTDDDGDGRVFNVLDTTGPTIINGYSQPGASPNTSPT
                     VSNAVIKIYIKGPRVANTSVRNYLSIDGFHVFSGGNEFRGLAFTHLRRSIWLSGHNAH
                     DNIVAGCF"
BASE COUNT          724 a         1405 c         1451 g          767 t
ORIGIN      
        1 gctcgcgagg agtccgatcg ccagcgccgc cttcttgatc tggcctgggt caaaccgtcg
       61 tgtcgttgtt gtcgcctgca ttgctggtct ccttctgggc tgctgttcag tggtgacgac
      121 cgcttcgtta accacaccca cagcatgcgt gaggagcagg caggccacat cccgcacgat
      181 aatgaatcgc gcttggagac cggctagtta tttctaatga gtcatccggt tcggtatctc
      241 taccggtggg ggcgatgggc gagacggtca gacgaggcca tgccggatgg cataggtcgc
      301 ggcagcggtc cgcgactcga caccgagctt attcatgatg ttcgcgacgt gggtcgcgac
      361 ggtgtgcggc gagatgtaga gcgcttcagc gatctcctgg ttggacgcac cattgaccag
      421 cagccgtagc acgtcgagct cgcgagcgct gacgcccagg ctggcggttt gcgtcgaaga
      481 cgccgaatcg ttggacacgt tcgttggacg cgcggaatcg agggcaaatg cgatcgcgtc
      541 cgccaccggc atcgcttggc cggcatgcca ggcggcctga tacgcgtccg cttccagtcg
      601 cgactggacc gacgcgatgt cgtgggcgag catctcaccc agaaaggctt cgacgaccgg
      661 catgccgatc gcggttcgca atccatccgc cgcgccgaag agacgcgcgg cccgctcgag
      721 gtcgccccgg cgagcggcga tgcgtccgaa gccttcgaag gagagcgcga tgaaacgggt
      781 atcgtcgatc tccaggcaga gtctgaccgt ctcggtgaag aggctggttg ctccgtcgag
      841 gttcccggtg tcgagcgcgt tgtacgccag ccccgctgtg tagttggcga tcccgggcgt
      901 actttgcagg tcgcgggcga gagcgagggc ctcttcaagc atggcgcggc cgcgttccag
      961 gtcgccagtg ccgagtgcgt tccagcccag attgccgacg gcaccttgta ttccaatcgg
     1021 atcgtttagc gtccggaaga tggcgaggct ctcttcgccg atccgggctg ctcgttcaaa
     1081 gtcgccctcc gccatcgccg accggcacat gccgcggagg gtgttggcga tgttccagtc
     1141 gtcgccgagc gtgcggaaga tggtgaggct ctgctcaaac agctcatccg cccgcttgaa
     1201 gtcaccctgc cacgacgcga ggctggcggc tgtgtcgagg ccgctggcac gcaccggatc
     1261 ggagacgatg acatccgccg cgagggcggc ttcgagcacc tggcgaccgt cggtgaagtg
     1321 cgcgtgggtg agccagaagt tccagagagc ggcggcggcg cggacagcgg tctcgacatc
     1381 atggtcgagc gcccagtcga gcgcgaggcg caggttgctg tagtcggcgt cgagccggcg
     1441 cagccattcc atcccaccgg cgcgcatcaa gtgctgtcca gcctcgatgg caagtgcaag
     1501 gaagtagcgg gcatgccgtt cgcggatctc ctcctgctcg ccgtgtgcct gaagctggtc
     1561 gagcgcgaac tcgcgaatcg tctccagcac gccgaagcgg atcgcgccgt cgggttgctc
     1621 aatctggcgg atcaggctgt ggtcgacgag agccgccagc cgctcgaaca gctcctcctg
     1681 gccgcagacc gcttcggcgg cctccagcgt ccagccgccg aggaacgccg agagcgcgcg
     1741 gaacatgcgt tgctcgtcgg acgccagcag gtcgtagctc caggcgatcg tgtcgcgcat
     1801 cgtgcgttgc cgggcgggaa ggtcgcgcgg cccgccgctg agcagcggca gccgctgttc
     1861 gagccggtcg agtagcgccg gcggtgacag catcttcgtt cgggcggcag ccagttcgat
     1921 cgcgagcggc agcccatcga gccgccggca gatctcagtg atgatcgcgg gggtgtcgtt
     1981 cgtcgtcgcg atctcggggt tgacgcgcgc ggcacgctcg acgaagagcg ctgtcgcgcc
     2041 gtacgccgcc agatcgacgc aggatgaggg actgtcggaa gcgggcaggt ccagcggacg
     2101 gagcgggtac tcgcgctcgt cgtggaggcg tagcggcgtc cggctggtgg cgaggacgat
     2161 cagctcccgg caggccgcga tcagctcgcc gacggccgtc gctgcctcga tgacgtgctc
     2221 gacattgtcc agcacgagca gcagtcgccg gttcccgatg gcggagatga cctggttgag
     2281 caggatctga ccgggctgct cgctgatccc aagtgtgctg gcgatggctg ggacgacaag
     2341 agcggcatcg ttgagtggcg cgagctggac gaaccggatc gcatccggga acctatcgtt
     2401 gagcgcggcg gctaccgcca gcgcgagttg cgtcttgcca accccgcccg gaccaatcag
     2461 cgtgaccagc gccacgtcct cttgctgcaa cagcccagtg agcgtcgcca gctccctctc
     2521 acggccgatg agccgcgtgc ggatgcgcgg cagcgtcgta acctcatccg gccgtgcggc
     2581 atctggcatg aacgggactc aattccaggt tggcagcagc gttgtcccta tcatcgtgct
     2641 gagatgcaac gtcgtcaagc actcctgtca tgtgtgttca aatctggcgc gattgtggag
     2701 gcggccgtac ctggttgcat ccgactcgct gtagaggtcg atgttcagac tgaacgattt
     2761 gctcaaagcg aactgtattg attctggtga ggatcatata gcccaaatgg gctatacgca
     2821 tggtacgata cggacaacca caacgtgtct tgcgcaatgt cttggaatgt tgttggagat
     2881 gtccggtcgc ggaagggtat cgcgtgggcg cgaaatatat acatcgcctc tcaatgaggc
     2941 gatcgatcgc tatgatgtgt tgtctcgtcg cgattctggc gggagttgcc tggggaatcg
     3001 gctggcagtc cggtagcgcg gccagcccga agctcgcgat agacgcgcct gaggaggcgc
     3061 tggccggcga gccgatcgag atccggctcg tgatcgacga cgccaacggc atcgccgcct
     3121 ttgacgcgac gatcggcttc gatacctcga tcgccagcct gcaatcgttc gatcagcgcg
     3181 cgaatgacct ggtcgacctc ggccgcgacg tcaaggcgct ggggccggtc gaactgccgg
     3241 atggcgtggc ggtcggcgcc tacgcctgtc ctgctgaaag ctgcgctgac ctggccggca
     3301 agagccgcaa caccgcccag ggcacgggta gcttccggct ggcggccttc acgatcgtcg
     3361 gctggcagcc cggcccgctg acgatcgacc tgaccacggc ccagttcacc gacggcagcg
     3421 gccggccggt tgaggtcgat ctctccggcg cgaccgtgac cgtcgaggtc atgccgaatg
     3481 gcgaggtgca gtagcatgac gcacctcttc tcccgcacca cccgcctgct gctggctgcc
     3541 ttgaccgtcg ttgccttgct gctctcgctg ctgccggccg gccagctggc cgcccagccc
     3601 gccaacgtcg atccatcgct cgcgtacctc gacctggact ccgacggcca gatcaccgag
     3661 atcgacctga tgtcggtcac gaccgcctgg gagtcgcgca agctcagcgg cgacgcctgt
     3721 tccggcccgg ccgccgccga tgtcaccggc gacggctgcg tgacgatcgc cgatgtccag
     3781 acgctggccg ccaatctggg cgtcagcgtc ctcgggccgt ccgcgatggc cgagcggtcg
     3841 cgggcgatgg tctccagcga cccgaccttc gtcgtcacga cgaccagcga cgcgccggac
     3901 agcaaccccg gcgacggcgt ctgccagatc gccggcggcg gctgctcgct gcgggcggcc
     3961 atcgacgagt ccaatcggat gcagggcgcg aacaccatca atttcaatat ccccggctcc
     4021 ggcgtgcacg tcatcaacgt caccgacgac gacggtgatg gacgcgtctt caacgtgctg
     4081 gacacgaccg gcccgacgat catcaatggc tactcgcagc cgggcgcatc gccgaatacc
     4141 agcccgaccg tcagcaatgc cgtcatcaag atctatatca agggaccgcg tgtcgcgaac
     4201 acgtcggtcc gcaactacct cagtatcgac ggcttccacg tcttctcggg cggcaacgag
     4261 ttccgcgggc tggcgttcac ccacctgcgc cgctcgatct ggctcagcgg ccacaacgcg
     4321 cacgacaaca tcgtcgccgg ctgcttc
//
LOCUS       DASUGJ010000847         6777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1805819, whole genome shotgun sequence.
ACCESSION   DASUGJ010000847 DASUGJ010000000
VERSION     DASUGJ010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805819"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VFV93_18975"
     CDS             complement(<1..800)
                     /locus_tag="VFV93_18975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5167501.1"
                     /translation="MTLATRIHGNEDPTYWLNRNQAETNRLLLQSRLINPITRAILVE
                     AGLASGMTVLDIGSGPGDVALLAAKIVGPTGKVVGVDKDPAILEVARRRACAAGLTNV
                     HFIDGDVRSVEPGASFDAITGRLVLLYLGDPVATLAALTERLRPGGIAAIQEANIRPE
                     TVYAHPQLGIWEKVSSWVGAAAGAAGLNQTIGFDLRRIFLSAGLPEPSMRFDSPLGGG
                     MDWAGYDYIAATVRNMLPLILKNRIDSADEIDISTLADRLRIATTAAD"
     gene            complement(1019..1690)
                     /locus_tag="VFV93_18980"
     CDS             complement(1019..1690)
                     /locus_tag="VFV93_18980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5167502.1"
                     /translation="MSDEPIRILLIDDHASTREPLAFMLNQEPDLTVVAQAGSLAEAR
                     VAIDDTGLALDLAIIDLGLPDGDGEELIVDLRSANRETTALVLTYFSEQDRLARAVAA
                     GAAGVLHKSAPVQEVIDAVRRLHAGEQLVTVTDVIDAIQRLDLERQRDRERHLTYEQL
                     TQRELDVLQALAEGLSDKDISERFQISAATVRTHVNNILTKLYATSRLQALVTAVRNG
                     IVEIA"
     gene            complement(1696..3258)
                     /locus_tag="VFV93_18985"
     CDS             complement(1696..3258)
                     /locus_tag="VFV93_18985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5167503.1"
                     /translation="MLTEGGTIAVALALAAAVVGWALINLALGWVGFATIVPLGFRGG
                     MEAAISLARLFAALVLILIIADGLGNRLRWLACGFVVLGLGQLIFGYIEPIVVNSTEI
                     NTALYEMILVRSLAGALFVAGLVPRVPPRFTTRTAVTVLAVALACAAGYRLLGAADRI
                     PELVQVESLEEAARLKIAPMSWMTSWHWAFAAIPLGLSIVAAIGALRRNRARDVGAWL
                     PFSIVLLAGSELHDALWPSAYGNSVLMNTADVLRLTMAAVVVVGGALELRRLANERAT
                     LLTAVRDRAHQLEELATLKADFTTMIAHELGHPLSAIRRQAELLGRDGLDPTMRQATI
                     TTIVHETDALDALVADVQATATVERPDFAVALRPVLIGDVLRDAAGSAEAHSRERHLA
                     TELVGVQPHERLLADPERIGQVLRNLLCNAAKYTPDGTRITLRFSDAGPARVGVDVAD
                     DGPGIAPDDLNRIFEKYGRGRMRENGQIAGAGIGLYVSRRIVRAHGSDLTVVSQPGNG
                     AVFSFELSRTES"
     gene            3609..6752
                     /locus_tag="VFV93_18990"
     CDS             3609..6752
                     /locus_tag="VFV93_18990"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX5167504.1"
                     /translation="MDQKLLNSLEWRLVGPHRGGRVVAVAGHPAKREVFYFGACAGGV
                     WKTTSAGALWENVSDGYFKTSAIGALTVSDADPNVIYAGTGETSIRSNVSHGDGVYRS
                     IDGGHTWQNLGLADTRHIGDILVHPKNPDHVFVAALGHAYGRNEERGVFRTTDGGKHW
                     EKVLYISDKAGVHDIAWDPNNPRILYAAAWETQRHPHALWSGGEQSGIWRSTDGGDTW
                     SEITRNPGMTEDAVLGKIGVAASPARPGRVWAIVEAENGGGLYRSDDYGDSWTLLNGN
                     PDLRRRPWYYMHVFADPVDENTVWVLNVNCWRSHDGGKTFDQIPTPHGDNHGLWIDPK
                     DSNRVIEGNDGGACITYDGGYTWSSILNQPTAQFYHVTADNETPYNIYGSQQDNWAMR
                     LPSIGFEGAISQKDYMEPGGGESGHIAISRKEPHTVFGGGIGTGLGHGRLIAWNPQTR
                     HARNVTVWPEVFGFGAGADQLKYRFQWTFPVEFSPHSDDVLYACSNVVHKSTDEGSSW
                     QVISPDLTRNDPDRTKSSGGPITADNSGAEIYCTIFAFRESPLEEGLLWAGSDDGLIH
                     VSRDGGKNWENVTPKDYPEWTRISIIEPSPFDAGTVYVAANNYQQDDLKPYLLRTNDY
                     GKTWKLITNGIPDDEFTRTIRADPARQGLLYAGTEVGLHISFDDGDSWFPFQSNLPVT
                     PIHDVIVKGTDLVAATHGRSFWILDDLSPVHQMKPSTAEEPVVLFQPRDTIRYRHYGR
                     AFGATPDITNYKMTGPTTVAYRLEKTAMGTNKEVFLDAGQNPPNGVVIHYWLKEKPEG
                     ELTVTILDADGNEVRTYSSTSEDPPKAPAAVGANRLVWDYRYASPTKLIDPPKIDRFG
                     QMMEAGAAPRGVPGTYQVRLKVGDTELTQPFTILDDPRLSVTQKELAEQFDLKLTIRN
                     DISTVHEALNQLSAIKKQVEAWEGLLKDDPNREDVAKVAAEVKEALVEVEGELVNLDS
                     DKPQPGSSRLKEKLIALSMMIDESDHAPTAGAVEVQAMLGDQLETVQARLRQVISSDV
                     QKLVKMLESAKIPRIVA"
BASE COUNT         1230 a         2097 c         2353 g         1097 t
ORIGIN      
        1 ctgtcggcgg cggtcgtcgc gatcctcagc cggtcggcga gcgtcgagat gtcgatctcg
       61 tctgcgctgt cgatgcggtt cttgaggatc agtggcagca tgtttcgaac ggtcgcggcg
      121 atgtagtcgt acccggccca atccatccca ccgccgagcg gcgagtcgaa gcgcatcgat
      181 ggctccggca gcccggctga gaggaagatg cggcgcaggt cgaagccgat cgtctggttg
      241 agtccagccg cgccggctgc cgcaccgacc caactggaga ctttctccca gatgcccagt
      301 tgcgggtggg cgtagaccgt ttccggccgg atattggcct cctggatggc ggcgatgccg
      361 cccggcctga gccgctcggt cagcgcggcc agcgtcgcaa ccgggtcgcc gaggtagagc
      421 aggacgagcc tgccggtgat cgcatcgaag ctggcaccag gctcgaccga gcgtacgtcg
      481 ccgtcgatga agtggacgtt cgtcagaccg gctgcgcagg cacgccggcg ggcgacctcc
      541 aggatggccg gatccttgtc gacaccgacg accttgccgg tcgggccgac gatcttggcc
      601 gcgagcaggg caacgtcgcc cggtccggaa ccgatgtcga gcaccgtcat cccgctagcg
      661 agtcctgcct cgaccaggat ggcccgcgtg atcggattga tcagacggga ctgcaggagc
      721 agccggttgg tttcagcctg gttgcggttc aaccagtagg tcggatcttc gttcccgtgg
      781 attcgtgtgg cgagggtcat ggaagttctc cagttcacgt catcgggctg gacgttgcat
      841 tcatgatcgc ggctgttgtg tggatatgcg ccagcaaatt cgatgagtgc gaaggtcgtc
      901 agaatcacgg atgagttgcg gggtggtcag tgactgacga cagtgggagg gcggctctgc
      961 cctgcccttt gtcatccttc ggctcacgcc tcaggatgac aaggacgggg gcggcttgtc
     1021 aggcgatctc gacgatgccg ttgcggacgg ctgtgacgag cgcctggagg cgtgaggtgg
     1081 cgtagagctt ggtgaggatg ttgttgacgt gggtccggac ggtggcggcg ctgatctgga
     1141 accgctcgga gatgtccttg tcgctgagac cttcggccaa cgcctggagg acgtcgagtt
     1201 cccgttgggt cagctgctcg taggtgaggt ggcgttcgcg gtcgcgctgt cgttcgaggt
     1261 cgaggcgctg aatggcgtcg atgacatcgg tgacggtgac gagctgttct ccggcgtgga
     1321 ggcgccgaac ggcgtcgatc acctcctgga cgggtgctga tttgtggagg actccggctg
     1381 cgccggctgc aacggcccgt gcgagccgat cctgctcgga gaagtaggtc agcaccaggg
     1441 ccgtggtttc cctgttcgcg ctgcgcaggt cgacgatcag ctcctcgccg tcgccgtcgg
     1501 gcaggccgag gtcgatgatc gccagatcga gggccaggcc ggtatcgtcg attgccacgc
     1561 gggcctcggc cagtgacccg gcctgggcga cgacggtcag atccggctcc tgattgagca
     1621 tgaaggccag cggctcgcgg gtggaggcgt gatcgtcgat gagcaggatc ctgattggct
     1681 cgtccgacat ggcgactacg attctgtccg tgaaagctcg aacgagaaga ccgcgccgtt
     1741 gcccggttgg gacacgacgg tcaggtccga accgtgcgca cggacgatac ggcggctgac
     1801 atagagaccg atcccggcgc cggcgatctg cccgttctcg cgcatccggc cgcgtccgta
     1861 cttctcgaag atacggttga gatcgtcggg tgcgattccc gggccatcgt cggcgacatc
     1921 taccccgacg cgggcgggac cggcatcgga gaaccgtagc gtgatccggg tgccatccgg
     1981 cgtgtacttc gccgcattgc agagcaggtt acgcagcacc tgaccgattc gttcggggtc
     2041 ggccaggagg cgttcgtggg gctgaacgcc gaccagctcg gtcgccaggt ggcgctcgcg
     2101 ggagtgggct tcggcggacc cggctgcatc gcgtaacacg tcgccgatca ggacaggacg
     2161 cagggcgacg gcgaagtcgg gacgctcgac tgtcgccgtc gcctggacat cggcgacgag
     2221 cgcatcgagg gcatcggtct cgtggacgat cgtcgtgatg gtcgcctgcc gcatggtcgg
     2281 atcgagaccg tcacgtccga gcagttccgc ctgacggcgg atggccgaga gcggatggcc
     2341 cagctcgtgg gcgatcatcg tcgtgaagtc ggctttgaga gtggcgagct cttcgagctg
     2401 gtgggcacgg tcgcggacag cggtgagcag cgttgcgcgc tcgtttgcca gccgccgtag
     2461 ttccagcgca ccaccaacga cgacgacggc tgccatcgtc agccgaagga catcggccgt
     2521 gttcatcagg accgagttgc cgtaggccga tggccagagt gcgtcgtgca gctcggaacc
     2581 ggccagcagg acgatcgaaa acggtagcca ggcgcccacg tcgcgggcgc gattgcgcct
     2641 gagtgccccg atagcagcga cgattgataa tccaagcgga atcgcggcga aggcccagtg
     2701 ccagctggtc atccaggaca tcggtgcgat cttgagccgg gcggcctctt cgagactctc
     2761 cacctgcacg agttccggga tgcgatcggc cgcgccgagc agccgataac cggccgcgca
     2821 cgccagcgcg acggccagaa cggtcactgc cgtgcgggtc gtgaagcgcg gcgggacacg
     2881 cgggaccagc ccggcgacaa agagcgcacc ggccagcgac cggaccagga tcatctcgta
     2941 gagggctgtg ttgatctcgg tcgaattgac gacgatcggt tcgatgtagc cgaagatgag
     3001 ctggccgagg ccgagcacga cgaacccgca ggccagccag cgtagccggt tgcctagccc
     3061 gtcggcgatg atgaggatca ggacgagggc ggcgaacagg cgggcgagcg agatcgcggc
     3121 ctccatcccg ccccgaaagc cgagcggaac gatcgtcgcg aagccgaccc agccgagcgc
     3181 gaggttgatg agcgcccagc cgacgacggc agctgccagc gccagcgcga cggcgatcgt
     3241 cccgccttcg gtcagcaatg ccggtcgggt cgttgaggac tctgacgcga ctggtttcgt
     3301 ggcaggggta gataccggac cggcaggttc gcgatacaga cctggaattc gtgtcacgac
     3361 tttcgatgtc atgttgcacc gccgcacgac gtcgagtccg acaatccccg aggatagcac
     3421 gtgagtcaaa cggtgaccgg aagcagacag gacgtcgacg gcagccgttg tgaacgttca
     3481 cggtattgac agttgtccgg ttcgcgacaa gaatcccgag cagaactgcc cgccgggctg
     3541 gtgacgcagg gcgcgtcggc cggctggtta tgacgggcag ggacaaccca tgggaggagc
     3601 aaggaaacgt ggaccagaag ctgttgaact cgctggagtg gcggctcgtt ggcccgcacc
     3661 gcggcggtcg tgtcgtcgct gttgccggcc atccggccaa gcgtgaggtg ttctacttcg
     3721 gcgcctgtgc cggcggggtc tggaagacga ccagcgccgg cgcgctctgg gagaatgtct
     3781 cggacgggta cttcaagacg tcggcgatcg gcgcgctgac cgtctccgat gcggacccga
     3841 acgtgatcta cgccgggacg ggtgagacga gcatccgcag caacgtctcg catggtgacg
     3901 gcgtctaccg ctcgatcgac ggtggccaca cctggcagaa cctcgggctg gccgacacgc
     3961 gccacatcgg tgacatcctg gtccatccga agaaccccga ccacgtcttt gtcgcggcgc
     4021 tcggccatgc ctacggccgc aacgaggagc gcggcgtctt ccggaccacc gacggcggca
     4081 agcactggga gaaggtgctg tacatcagcg acaaggctgg cgtccatgac atcgcctggg
     4141 acccgaacaa tccccgcatt ctctacgcgg cggcctggga aacgcagcgc cacccgcacg
     4201 cgctctggag cggcggcgag cagtccggca tctggcgctc gaccgatggc ggcgatacct
     4261 ggagtgagat tacgcgcaac cctggcatga ccgaggatgc cgtcctcggc aagatcggcg
     4321 tcgccgcctc tccggcacgg ccgggccggg tctgggcgat cgtcgaggcc gaaaatggtg
     4381 gtgggctcta ccgctcggac gactacggcg atagctggac gctgctgaac ggcaacccgg
     4441 acctgcgccg gcggccgtgg tactacatgc acgtctttgc cgatccggtg gatgagaaca
     4501 cggtctgggt gctgaacgtc aactgctggc gctcgcacga cggcggcaag acgttcgatc
     4561 agattccgac gccgcacggt gacaaccacg gcctctggat cgacccgaag gactccaacc
     4621 gggtcatcga gggcaacgac ggcggcgcct gcatcaccta cgatggcggc tacacctggt
     4681 cgtcgattct gaatcagccg acggcgcagt tctatcatgt gaccgccgac aacgagacgc
     4741 cctacaacat ctatggttcg cagcaggaca actgggcgat gcggctgccg agcatcggct
     4801 tcgagggcgc gatctcccag aaggactaca tggagccggg tggcggcgag tcgggccaca
     4861 tcgcgatcag ccgcaaggag ccgcacaccg tcttcggcgg cgggatcggt accggcctgg
     4921 gccacggccg cctgatcgcc tggaacccgc agacgcgaca tgcccgcaac gtgacggtct
     4981 ggccggaagt gttcggcttc ggcgccggcg cggatcaact caagtaccgc ttccagtgga
     5041 ccttcccggt tgagttctcg ccgcacagcg acgatgtcct ctacgcctgc tcgaacgtcg
     5101 tccacaaatc gacggacgag gggtccagtt ggcaagtgat cagcccggat ctgacgcgca
     5161 acgatcccga ccgcaccaag tcgtccggcg ggccgatcac ggccgacaac tcgggcgcag
     5221 agatctattg cacgatcttc gcgttccgcg agtcgccgct ggaagagggg ctgctctggg
     5281 ctgggtcgga cgatgggctg atccacgtct cgcgcgacgg cggcaagaac tgggagaacg
     5341 tcacgccgaa ggactatccg gagtggacgc ggatcagcat catcgagccg tcgccgttcg
     5401 acgccgggac ggtctacgtc gcggccaaca actaccagca ggacgacctg aagccgtacc
     5461 tgctgcgcac gaacgactac ggcaagacct ggaagctgat cacgaacggg attccagacg
     5521 acgagttcac gcggacgatc cgcgccgatc cggcccgcca ggggctgctc tacgcgggca
     5581 cggaagtcgg gctgcacatc tcgttcgacg acggcgactc ctggttccca ttccagtcga
     5641 acctgccggt gacgccgatc cacgacgtga tcgtcaaggg caccgacctg gtggcggcga
     5701 cgcatggccg ctcgttctgg atcctggatg acctctcgcc cgtccatcag atgaagccat
     5761 cgacggccga ggaaccggtc gtcctgttcc agccgcgcga cacgatccgc taccggcact
     5821 acggccgggc gttcggcgcg acgccggata tcaccaacta caagatgacc gggccgacga
     5881 cggtggccta ccggctggag aagacggcga tgggcaccaa caaggaggtg ttcctcgacg
     5941 ccggccagaa tccgccgaac ggtgtcgtca tccactactg gctgaaagag aagccggagg
     6001 gtgagctgac cgtgaccatc ctcgacgcgg atggcaacga ggtgcgcacg tattccagca
     6061 cgagcgagga cccgccgaag gcaccggcag cggtgggtgc caaccggctg gtctgggact
     6121 accgctacgc cagcccgacc aagctgattg acccgccgaa gatcgatcgc ttcgggcaga
     6181 tgatggaggc cggggcggcg cctcgaggcg tgccgggtac gtatcaggtg cggctgaagg
     6241 tcggcgatac tgaactgacg cagccgttca cgatcctgga cgatccgcgc ctgtcggtca
     6301 ctcagaagga actggcggag cagttcgatc tgaagctgac gatccgcaac gacatctcaa
     6361 cggtgcacga ggcgctcaat cagctttccg cgatcaagaa gcaggtcgag gcctgggagg
     6421 gactgctgaa ggacgatccg aaccgcgagg atgtcgcgaa ggtagcggcc gaagtcaagg
     6481 aggcgctggt cgaggtcgag ggcgaactgg tgaatctcga ctccgacaag ccgcagcccg
     6541 gctcgtcacg gctgaaggag aagctgatcg cgctctcgat gatgatcgac gagtcggacc
     6601 acgcgcccac ggccggagcg gtcgaggtgc aggcgatgct cggcgaccag cttgagacgg
     6661 tccaggcgcg gctgcgccag gtcatctcca gcgatgtcca gaagctggtg aagatgctcg
     6721 aatccgcgaa gattccgcgc atcgtcgcct gacgaatgca gccgcagcga cgcggcg
//
LOCUS       DASUGJ010000848         4471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1526070, whole genome shotgun sequence.
ACCESSION   DASUGJ010000848 DASUGJ010000000
VERSION     DASUGJ010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526070"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VFV93_18995"
     CDS             complement(<1..1077)
                     /locus_tag="VFV93_18995"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5167505.1"
                     /translation="MSATGRQAAAPVPLIPRFAWSPKYADFPLPFPGSEAGSRPVGVS
                     ASPATTYPGAGQPFAWQKILPIAALALAVLAFGYFVLADGGGDDDDGQQGTGGNVTTT
                     ATSATGNATQRPGEGGAAATTTHAAGATATTGGVGSAGETEEPEATTGTGESPDDSEA
                     TEPSDEGIVDPADLGIIQVEAEEGETLIDIANEWGLEVSTLVWANGIEDPGMPLPAGT
                     LVTVPPFDGVIHEVAEGETLESIAALYGVAPVDIINITQNKVQSDADLHPGMLITVPY
                     ATPQTRGGLAEYTVKEGDDLWKIADYYGIDALTIAYANEIPQSFLIFPGQVLIIPPAD
                     GILYWVVEGDTVESIASAFSVDPSL"
     gene            1738..2967
                     /locus_tag="VFV93_19000"
     CDS             1738..2967
                     /locus_tag="VFV93_19000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX5167506.1"
                     /translation="MAVDTRTEIGVLRERVKEAINNERDHLVEVAETIRTNPELGYEE
                     RMASQLLADQLREAGFEVEKPYKGIETAFRATRKGGAGDGPTVAILAEYDALAGIGHG
                     CGHNLIGGSGLAAAIGLGAVIDEVGGTVVIMGTPAEEGGAGKVRIAEQGGFDDIDAAL
                     MIHHAGDRTGAPLQWPQGTCLALTDIAFEFFGKPAHAAADPYNGVNALNAVIETFNGI
                     NALRQHIVMDARIHGIITHGGEASNVTPKYARAAFLVRAGTQAYVAELAEKVRNIARG
                     AALMTGCELKIEEGTLHADMRPSYVLGERYHANMIDVGLDISHGREGRGMHSTDFGHV
                     SYKLPSVTGSFAISKTPIPGHSQQVVDASGSEFGYDQYIKVSTAMALTALDILTDPDL
                     SKQAWEKHKRWSEEYEG"
     gene            3031..4257
                     /locus_tag="VFV93_19005"
     CDS             3031..4257
                     /locus_tag="VFV93_19005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX5167507.1"
                     /translation="MTATTDVATLRDRVLEAIENEREHIVEVSETIRVNPEYQYEEFM
                     ASQLLADQLREAGFEVEKPYKGLETAFCATRKGAAGGGPTVAILAEYDALRGIGHGCG
                     HNLIGASGLAVAIGIGSVIDETGGTVIIMGTPAEEGGGGKVKLLEQGAFEGVDAALMI
                     HHAGNRAGAASNYPDGTNLATTGFTFEFFGKPSHAAADPENGVNALNAVIETFNGINA
                     LRQHVTMDARIHGIVTHGGDAVNVTPKYARCQFGVRAARTDYLKELADKVVKIAEAAA
                     QMTGCEIKVTERDTPYLERRPSYVLGRKYEDNMRAVGQDMTNPARGRTWASTDFGNVS
                     HHVPSVTGSFPISTEPIPGHSQQVVDASGSEYGYDQFIKVSKAMALTTLDILTDPELG
                     KEAWDEQKNWLKLYGG"
BASE COUNT          838 a         1491 c         1439 g          703 t
ORIGIN      
        1 cagcgacgga tcgactgaga aggcagacgc aatcgactcg accgtatcgc cctcgacgac
       61 ccagtagagg atgccgtcgg ccggcgggat gatcaggacc tggccgggga agatcaggaa
      121 gctctgcgga atctcattgg cgtaagcgat cgtcagcgcg tcgatgccgt agtaatcggc
      181 gatcttccag aggtcgtcac cctccttgac cgtatactcc gccaacccgc cacgcgtctg
      241 cggcgtcgca tacggaaccg tgatcagcat gccggggtgc aggtcggcgt cgctctgaac
      301 cttgttctgg gtgatgttga tgatgtcgac cggcgcgacg ccgtacaggg ccgcgatcga
      361 ctccagcgtc tcgccctcgg cgacttcgtg gatgacgccg tcgaacggag gaaccgttac
      421 aagcgttccg gccggcagcg gcatgccggg gtcttcgatg ccattcgccc agacgagcgt
      481 cgagacttcc agcccccact cgttcgcgat gtcgatcagt gtctcgccct cttcggcctc
      541 gacctgaatg ataccgagat cggcgggatc gacgatgcct tcgtcagacg gctcggtcgc
      601 ttcggaatcg tccggcgatt caccagtgcc ggtcgtcgct tccggctcct cggtctcgcc
      661 agcactgccg acgccgccgg tcgtcgccgt cgcgccggcc gcatgagtcg tcgtcgcggc
      721 cgcgccacct tcgccggggc gctgggtggc gttgccggtc gcgctggtgg ccgtcgtcgt
      781 gacgttcccg ccggtgcctt gctggccgtc gtcatcgtcg ccaccgccat cggccagcac
      841 gaagtagccg aacgcgagaa ccgccagcgc cagcgcggcg atcggcagga tcttctgcca
      901 tgcgaacggc tggccggcgc cagggtaggt cgtggccggc gaggcgctga cgccaactgg
      961 ccggctgcct gcctctgagc cgggaaatgg caacgggaaa tcggcatact ttggtgacca
     1021 tgcaaagcgc gggatgagtg gaacgggagc agccgcttgt cgcccggtcg cgctcaccgg
     1081 cggggtccgt ccggcaggcg gactaccacc agaaagctgt cctgatcgtt gatttcgagc
     1141 gtccacacga ctactccgtc ccaggttctg cggccctcgc gcgtggcgcc ggccccctgt
     1201 ctcaaccccc accggatcgc cgaggaaccc gcgcgcaggg tgtccgtaca cctgtgacca
     1261 gcatagcacc tcgcttgcgc ttggtcaatt tttcctcgct gtcacaggtc ggggcgacgg
     1321 gctgacgaga ttccgcatgt gtacgtacat tcggtcgttt gggacggctg cgatggcgac
     1381 agcctgtcac agtgttgcag aatccgcgat ccgacaggcg ctgtcaaccc cgccatgtca
     1441 atcccacaag tcgtcgtcac acattccgtc acacggcagt ggcaatccac tactgtacgt
     1501 acactcggct ggcgactgtg cgttgacaac acgaccggcg tgcctcaatt tgcgtgccgc
     1561 gggtgttggg gattcgggac acgggaatcg gggatggtga gacacctcgt cgaacatctc
     1621 cgaatcccgt gtcccgaatc ccgattcccc aacacccgca ccgtagcgtc cgccgtcgct
     1681 ccgtgctagt ctcccgccag cacgataccc gcccagcaga gagggaggat ggacaggatg
     1741 gctgtcgata cacggacgga gatcggcgtc ctgcgcgagc gcgtcaagga ggcgatcaac
     1801 aacgagcgcg atcatctggt cgaggtggcc gagacgatcc gcaccaaccc cgagcttggc
     1861 tacgaggagc ggatggcctc acaactgctg gccgaccagc tccgcgaagc cggcttcgag
     1921 gtcgagaagc catataaagg aatcgagacg gcctttcggg ccacccgtaa gggtggagcc
     1981 ggcgacggcc caaccgtcgc gatcctggct gaatacgacg cgctggccgg catcggccac
     2041 ggttgcggcc acaacctgat cggcggctcc ggcctggcgg cagcgatcgg cctcggggcc
     2101 gtcatcgacg aggtcggcgg gacggtcgtc atcatgggca cgccggccga agagggcggc
     2161 gccggcaagg tccggatcgc cgaacagggc ggattcgatg atatcgacgc ggcgctgatg
     2221 atccaccacg ccggcgacag gaccggcgcg ccgctccagt ggccgcaggg cacctgcctg
     2281 gcgctgaccg acatcgcctt cgagttcttc ggcaagccgg cccacgccgc cgccgacccc
     2341 tacaacggcg tcaacgcgct gaacgcggtc atcgagacgt tcaacggcat caacgccctc
     2401 cgccagcaca tcgtcatgga tgcccgtatc cacggcatca tcacgcatgg cggcgaggcc
     2461 agcaacgtca cgccgaagta cgcgcgggcc gccttcctcg tccgggccgg cactcaggcg
     2521 tacgtcgccg agctggccga gaaggtgcgc aacatcgccc gcggcgcggc actgatgacc
     2581 ggctgcgagc tgaagatcga agagggcact ctccacgccg acatgcggcc atcgtatgtg
     2641 ctcggcgagc gctaccatgc caacatgatc gatgtcgggc tggacatcag ccacggccgc
     2701 gagggacgcg ggatgcactc gaccgacttc ggccacgtct cctacaagct gccatcggtc
     2761 accggcagct tcgccatctc caagacgccg atccccggtc actcgcagca ggtcgtcgac
     2821 gcctccggca gcgagttcgg ctacgaccag tacatcaagg tctcgacggc gatggcactg
     2881 acggcgctcg acattctgac cgacccggat ctgtcgaagc aggcatggga gaagcacaag
     2941 cgctggtctg aggagtatga ggggtaatcc ctccccccag ccccctcccc tacagggagg
     3001 gggagtacga atagcgaaga ggagattgag atgacggcga caaccgacgt ggcgacgttg
     3061 cgggatcggg tgctggaggc gattgagaac gagcgggagc acatcgtcga ggtcagcgag
     3121 acgatccgcg tcaaccctga gtatcaatat gaagagttca tggcctcgca actgctggcg
     3181 gaccagctcc gcgaggcggg cttcgaggtc gaaaagccgt acaaggggct ggagacggcc
     3241 ttctgcgcca cccgcaaggg cgcggccgga ggcggcccga cggttgcgat cctggccgag
     3301 tacgacgccc tgcgcgggat cgggcacggc tgcgggcaca acctgatcgg cgcctcgggg
     3361 ctggcggtcg cgatcggaat cggctcggtc atcgatgaga cgggtgggac cgtcatcatc
     3421 atgggtacgc cggcagagga gggcggcggt ggcaaggtca agctgctgga gcagggcgcc
     3481 ttcgagggcg tcgatgccgc gctgatgatt caccacgccg gcaaccgcgc cggtgccgcc
     3541 tcgaactacc ccgacggcac caacctggcg acgaccggct tcacgttcga gttcttcggc
     3601 aagccgtccc acgcggcggc cgaccccgag aacggcgtca acgccctcaa cgccgttatc
     3661 gagacgttca acggcatcaa cgcgctgcgc cagcacgtca cgatggatgc ccgcatccac
     3721 gggatcgtga cgcacggcgg cgatgccgtg aacgtcacgc cgaagtacgc ccgctgccag
     3781 ttcggcgtcc gcgcagcccg aaccgactac ctcaaggaac tggccgacaa ggtcgtcaag
     3841 atcgccgagg cggctgcgca gatgaccggc tgcgagatca aggtcaccga acgggacaca
     3901 ccctaccttg aacgccgccc cagctacgtc ctcggccgca agtacgagga caacatgcgc
     3961 gccgtcggtc aggacatgac caacccggcc cgtggccgga cctgggcctc gaccgacttc
     4021 ggcaacgtct cccaccacgt cccgtcggtc accggcagct tcccgatcag caccgagccg
     4081 atccccggcc actcccagca ggtggttgac gcctccggct cggagtacgg ctacgatcag
     4141 ttcatcaagg tctcgaaggc gatggcgctg accacgctcg acatcctgac cgacccggag
     4201 cttggcaagg aagcctggga cgagcagaag aactggctga agctgtacgg cgggtaaggc
     4261 agactcacct ccagcccctc ccctacaggg aggggaagca cgctacttgc aacgaaaagc
     4321 cccctccccg agtcggggag ggggaaccac aagctgtcgt cattaggaaa atggaatcgg
     4381 taatggcagt tgtggcttga ttttcggcca ccacacgccg atgactaccc aacttttcgg
     4441 atggtatcca agcgtaccaa caaacaagtg c
//
LOCUS       DASUGJ010000849         5689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_694036, whole genome shotgun sequence.
ACCESSION   DASUGJ010000849 DASUGJ010000000
VERSION     DASUGJ010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694036"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFV93_19010"
     CDS             <1..235
                     /locus_tag="VFV93_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167508.1"
                     /translation="VAVRDGTVIMQAGGGIVYDSTADFEYQETLNKMGAAMRALEVAE
                     EIEEAVRDAGRGMRDAERSDEAVAGALVPEVVA"
     gene            232..834
                     /locus_tag="VFV93_19015"
     CDS             232..834
                     /locus_tag="VFV93_19015"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX5167509.1"
                     /translation="MILLIDNYDSFTYNLYQYLCELGAEVQVARNDVLSVHDVRTLQP
                     ERIVISPGPCTPDEAGISVDLIQELAGQVPILGVCLGHQAIGAAFGAEIVRAPQVMHG
                     KTSSISHDGRGIFSGLPNPFTATRYHSLIVEEASLPDCLEVTARSEDGIIMGLRHREC
                     DISGVQFHPESILTTGGHRLLANFIGAGSHHAAAASPIGY"
     gene            865..1914
                     /gene="trpD"
                     /locus_tag="VFV93_19020"
     CDS             865..1914
                     /gene="trpD"
                     /locus_tag="VFV93_19020"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5167510.1"
                     /translation="MLKPHIQAVVEGETLSREEARRAMEIIMNGEASAAQIGSFLMGL
                     RMRGETDDELAGFAEVMRAKAVRVPLGEIGPVIDTCGTGGDGAHTFNISTTAAFVVAG
                     AGVKVAKHGNRAATSRCGSADVLEGLGVKIELAPEQVAEAIERVGIGFMYAPAFHPAM
                     RFAGPVRREIGVRTVFNLLGPITNPAGAQHQVIGVPTAEAAARLARVLGLLGSRHALV
                     VHAHEGLDEIGVAGGTTVAEMLMRESAWVTTYDVTPEQYGFERRGRDAVRGGSVADNV
                     EIVNAVLAGERGAHRDIVLLNAGAALYAADIVPNIATGIQVASESIDSGAARERLDAL
                     VALTMRMGVAEKAAA"
     gene            1914..2714
                     /gene="trpC"
                     /locus_tag="VFV93_19025"
     CDS             1914..2714
                     /gene="trpC"
                     /locus_tag="VFV93_19025"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX5167511.1"
                     /translation="MVGATTGTYLDRILENTAAELARRKPLRTLAEIEQAAAAQPAAI
                     PFAAALRGSGVSVIAEVKRASPSKGAIAPDIIATDVAREYIAGGAAAISVLTDERFFR
                     GTIEDLEAVAALGRETRTPALRKDFVIDSYQIAEAKASGSAAVLLIVAALELARLREL
                     LSATAGYGLEALVEVHDEQELETALSVGATLIGINNRDLRAFTVDLATTERLAAQVPE
                     GNTLVGESGVRTRADVERLGRAGVDAVLVGETLMLAPDRAAAVRELLR"
     gene            2711..3385
                     /locus_tag="VFV93_19030"
     CDS             2711..3385
                     /locus_tag="VFV93_19030"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX5167512.1"
                     /translation="MTLIKICGIREAEHALVAIEAGADMLGLVFYPPSPRFVTIEQAR
                     AVRDAVREADPDRRVRLVGLFVNEPPGRMNAVAAEVGLDLIQLSGDEPPSVIAELDRP
                     AIASIRIDSSGKQDEERRFRELTAAGPLAVHVDAHVPGMYGGTGTVADWFVATDFAQR
                     YPVILAGGLTPETAVDAVLRVKPFAVDVSSGVETNGVKDSSKIRVFIAAARSADRSTA
                     NAGTNR"
     gene            3439..4623
                     /gene="trpB"
                     /locus_tag="VFV93_19035"
     CDS             3439..4623
                     /gene="trpB"
                     /locus_tag="VFV93_19035"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX5167513.1"
                     /translation="MPDEFGRFGEFGGVYAPETLMPAIRELADAYATTKSDLAFQAEL
                     ETLQRNYIGRPTPLTLAANLSDHWGGARVYLKREDLAHTGAHKINNAIGQALLARRMG
                     KRRIIAETGAGQHGVATATACALLGLDCIVYMGEVDIRRQSLNVFRMKLLGAEVRSVT
                     SGSRTLKDAINEAIRDWVTNVDETYYLLGTAAGMHPYPTIVRDFQAVIGREAREQIIA
                     QTGGLPKAVVACIGGGSNAMGAFYAFIPDDGVELVGVEAAGYGLETGQHSAALTAGSV
                     GVLHGSKSYVLQDEHGQILEAHSISAGLDYPGVGPELSYLKSIDRLRTGGVTDTEALE
                     GFKLLCETEGIIPALESSHAIYYAASVAQSYARDDTIVINLSGRGDKDMHTVAEALGV
                     TL"
     gene            4620..5459
                     /gene="trpA"
                     /locus_tag="VFV93_19040"
     CDS             4620..5459
                     /gene="trpA"
                     /locus_tag="VFV93_19040"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX5167514.1"
                     /translation="MSTTETTRTQHSTLSTQHSRIADTFARAKAENRAALMPFVTVGW
                     PKLGDTERLVPALIEGGGDMIELGFPFSDPIADGPTIQQTNQQALANGVTPAYALEVA
                     GKLRANGVEAPLLFMGYYNPIFSYGLQAFAAACAGAGIDGLIIPDLPPEESDPLLDAC
                     REHGLNLIYLIAPTSTAERVDAVIERANGFIYIVSLTGVTGARSQLATNLADYIARVR
                     GKTDLPLAIGFGISTREHVEQVEPLVDGVICASALLNRLNGVASEELPRVAREFVEGL
                     RGT"
BASE COUNT         1007 a         1821 c         1977 g          884 t
ORIGIN      
        1 ggtcgccgtc cgcgacggga ccgtcatcat gcaggccggc ggcggcatcg tctacgactc
       61 gacggccgac ttcgagtacc aggagacgct gaacaagatg ggcgccgcga tgcgggcgct
      121 ggaggtcgcc gaggagatcg aggaggcggt gcgggacgcg ggacgcggga tgcgggacgc
      181 ggaacgtagc gacgaggccg tagcgggcgc actggtgccg gaggtggtgg cgtgattctg
      241 ctgatagaca actacgactc cttcacctac aacctctacc agtatctctg cgagctgggc
      301 gccgaagtgc aggtggcccg caacgatgtg ctcagcgtcc atgatgtgcg gacgttgcag
      361 ccggagcgga tcgtcatctc gccggggccg tgtacgccgg acgaagcagg tatctccgtc
      421 gacctgatcc aggaactggc cggccaggta ccgatcctcg gcgtctgcct cgggcatcag
      481 gcgatcgggg ccgcgttcgg tgcggagatc gtccgcgcgc cgcaggtgat gcacggcaag
      541 acaagcagca tcagccacga cgggcgcggc attttcagcg gcctgcccaa cccgttcacc
      601 gccacccgct accactcact gatcgtcgag gaggctagcc tgccggactg cctggaagta
      661 accgcccgct cggaggacgg catcatcatg ggtctacgtc accgcgaatg cgatatcagc
      721 ggcgtccagt tccaccccga gtcgatcctg acgacgggcg gccaccggct gctggccaac
      781 ttcatcggcg ccggcagcca ccacgcggcg gccgccagcc cgattgggta ttgagatgta
      841 ccgggagata gaaaggaccg cgacatgctg aagccgcaca tccaggcggt cgttgagggg
      901 gagacactgt cgcgcgagga ggcgcgacgg gcgatggaga tcatcatgaa cggcgaggcg
      961 tcggcggcgc agatcggctc gttcctgatg ggactgcgca tgcgcggcga gacggacgac
     1021 gagctggccg ggttcgcgga agtgatgcgg gccaaggccg tgcgcgtccc gctcggcgag
     1081 atcgggccgg tcatcgacac ctgcgggacc ggaggcgatg gcgcccatac gttcaacatc
     1141 tcgacgacag cggccttcgt cgtcgccggg gctggagtca aggtcgccaa gcacggcaac
     1201 cgtgcggcca cgagccgctg cggttcggcc gacgtgctgg aggggcttgg cgtcaagatc
     1261 gagctggcgc cggagcaggt ggccgaggcg atcgagcggg tcgggatcgg cttcatgtat
     1321 gcgccggcct tccacccggc gatgcgcttc gccgggccgg tccgacgcga gatcggcgtt
     1381 cggaccgtct tcaacctgct cgggccgatc acgaacccgg ccggcgcgca gcatcaggtg
     1441 atcggtgtcc cgacggccga ggcagcggct cgcctggcgc gggtgctggg gctgctcggc
     1501 tcgcgccacg cgctggtcgt ccacgcccat gaggggctgg acgagatcgg cgtcgccggc
     1561 gggacaaccg tcgccgagat gctcatgcgc gagagcgcct gggtgacgac ctacgacgtg
     1621 acgccggagc agtacggctt cgagcggcga gggcgagacg cggtccgggg tggcagcgtg
     1681 gccgacaacg ttgagatcgt caacgccgtt ctggccggtg agcggggcgc gcaccgcgac
     1741 atcgtcctgc tgaatgccgg ggcagcgctt tacgcggccg atatcgtccc gaacatcgcg
     1801 accggcatcc aggtcgcgtc ggagtcaatc gacagcggcg cggcccgcga gcggctggac
     1861 gcgctggtcg cgctgacgat gcggatgggc gtcgccgaga aagcagctgc ctgatggttg
     1921 gggcgacgac cggaacctat ctggaccgga ttctggagaa cacggctgct gaactggctc
     1981 gacggaagcc gctccgcacg ctggccgaaa ttgagcaagc agcagccgcg cagccggcag
     2041 ccatcccgtt cgcggcggcg ctgcggggta gcggagtcag cgtcatcgcc gaggtcaagc
     2101 gcgcgtcacc gtcaaaaggc gcgattgcac cggacatcat cgcgaccgat gtcgcccgcg
     2161 agtacatcgc cggtggcgct gcggcgatct cggtcctgac cgacgagagg ttctttcgtg
     2221 ggacgatcga ggacctggag gcggtcgccg cgctgggacg cgagacgcga acaccagctc
     2281 tccgcaagga cttcgtgatc gacagctacc agatcgccga ggcgaaggca tccgggtcag
     2341 ccgctgttct gctgatcgtg gcggccctgg aactggcccg gttgcgcgaa ctgctgtcgg
     2401 cgacggccgg ctacggactc gaagcgctcg tcgaagtcca tgatgagcag gaacttgaaa
     2461 ccgcgctgag cgtcggcgcg acgctgatcg gaatcaacaa ccgcgacctg cgtgccttta
     2521 ccgtcgatct ggcaacgacc gaacggctgg cggcccaggt ccccgagggc aacacgctgg
     2581 ttggcgagag tggcgtccgg acgcgggccg atgtcgagcg gctgggccgt gccggcgtcg
     2641 atgccgtcct cgtcggcgag acgctgatgc tggcgccgga tcgggcggcg gctgtacggg
     2701 agctattgag atgacgctga tcaagatctg tggcatccgc gaggccgagc atgcgctggt
     2761 cgcaatcgaa gccggggccg acatgctcgg cctggtcttc tacccgccca gcccccgctt
     2821 tgtgacgatc gagcaggcgc gggccgtccg tgacgcagtc cgtgaggccg acccggatcg
     2881 acgcgtccgg ctggtcggac tgttcgtcaa cgagccgccg gggcggatga acgccgtcgc
     2941 cgccgaggtc gggctggatc tcatccagct ctcaggcgac gagccgccgt cggtgatcgc
     3001 ggagctggac cgtccggcga tcgccagcat caggatcgat tcgtccggca agcaggacga
     3061 agaacggcgc tttcgcgaac tgacagcagc cggtccgctg gccgtccacg tcgacgcgca
     3121 tgtacctggc atgtacggcg ggaccgggac ggtcgccgac tggttcgtgg cgaccgattt
     3181 cgcgcagcgc tacccggtca ttctggcagg cgggctgaca ccagagacgg ccgtcgacgc
     3241 ggttctgcgc gtcaagccgt tcgccgtcga tgtatcgagt ggcgtcgaga cgaacggcgt
     3301 caaggactcg tccaagattc gcgtatttat cgcggccgca cgttccgccg accgttcgac
     3361 agccaacgca ggaacgaatc gatgattggg gcaaccggga tgacatcgac agcaacgaaa
     3421 ccgattgaga caggttctat gccggacgaa ttcgggcgct tcggcgagtt cggcggggtc
     3481 tacgcgcctg agacgctgat gccggccatt cgcgaactgg ccgacgccta cgcaacgacc
     3541 aagagcgacc tggcctttca ggccgagctt gaaacgttgc agcgcaacta cataggaaga
     3601 ccgacaccgc tgacgttggc cgccaacctg tccgaccact ggggcggcgc gcgggtctac
     3661 ctcaagcgcg aggatctggc gcacaccggc gcgcacaaga tcaacaacgc gatcggtcag
     3721 gcgctgctgg cgcggcggat gggcaagcgg cgcatcatcg ccgagaccgg cgccggccag
     3781 cacggcgtcg ctacggcgac ggcctgtgcg ctgctggggc tggactgcat cgtctacatg
     3841 ggcgaggtcg atatccggcg ccagtcgctc aacgtcttcc ggatgaagct gctgggggcc
     3901 gaagttcgtt ccgtcaccag cggctcgcgg acgctgaagg acgcgatcaa cgaggcgatt
     3961 cgcgactggg tgaccaacgt tgacgaaacc tactacctgc tcgggaccgc cgcggggatg
     4021 cacccctacc cgacgatcgt gcgcgacttc caggccgtca tcggccgcga ggcgcgcgag
     4081 cagatcatcg cgcagaccgg cgggctgccg aaggcggtcg tcgcctgcat cggcggcggc
     4141 tcgaacgcga tgggtgcctt ctacgccttc atccccgacg acggcgtcga gctggtcggc
     4201 gtcgaagccg caggttacgg actggagacc ggccagcact cagccgccct gacggccggg
     4261 agcgtcggcg tcctccacgg ctcgaagtcg tacgtccttc aggatgagca cggccagatc
     4321 ctcgaagcgc actcgatctc agccgggctc gactatcccg gcgtcggccc tgaactctcg
     4381 tacctcaaga gcatcgaccg gctccgtacc ggcggcgtga ccgacaccga ggcactggaa
     4441 ggcttcaagc tgctctgtga gacggagggc atcattcccg ccctcgaatc gtcgcacgcg
     4501 atctactacg ctgcatcggt cgcgcagtcg tatgcgcggg acgacacgat cgtcatcaat
     4561 ctctccggcc ggggcgacaa ggatatgcac accgttgcgg aagcgttggg ggtgacactg
     4621 tgagcacgac cgagacgaca cgaactcagc actcaacact cagcactcag cactcccgca
     4681 tcgcagacac gttcgcccgc gccaaggccg aaaaccgtgc cgcgctgatg ccgttcgtga
     4741 cggttggctg gccgaagctg ggggataccg agcggctggt gccggccctg atcgagggcg
     4801 gcggcgatat gatcgagttg gggtttccgt tctccgatcc gatcgcggat ggtccgacga
     4861 tccagcagac gaaccagcag gcgctggcca acggtgtcac gcccgcctac gcgctggagg
     4921 tcgccgggaa gctgcgggcc aacggcgttg aagcgccgct gctgttcatg ggatactaca
     4981 atccgatttt cagctacggg ctccaggcgt ttgccgctgc ctgtgccggt gccggcatcg
     5041 acgggctgat catccctgac ctgccgcctg aggaaagcga cccacttctc gatgcctgcc
     5101 gggaacacgg cctcaacctc atctacctca tcgctccaac cagcacagcg gagcgggtcg
     5161 acgccgtcat cgagcgcgcc aacggcttca tctacatcgt ctcgctcacc ggcgtcaccg
     5221 gcgcgcgcag ccagctggcg accaaccttg ccgactacat cgcgcgggtg cgggggaaaa
     5281 ccgacctacc gctggccatc ggcttcggta tctccacccg cgagcacgtc gaacaggtgg
     5341 agccgctggt cgatggcgtc atctgcgcga gcgcgctcct caaccgtctt aacggagtcg
     5401 cgtctgagga gctgccacgg gttgcgaggg agtttgtcga aggattacga gggacgtgag
     5461 tcgggattcg ggaactggga ttttggagag ggagtacgac gtacaccatc ccgaatcccg
     5521 agtcccgaat cccgataacg agagggagac catgaccgtt tgccgggcca ttcggggggc
     5581 gacgaccgcc gagcagaata ctgccgagga catcatcgag gcgaccgagg agctgttgcg
     5641 ctcgatcctg cactacaacg agctgcaggt tgaggatctg gtcagcatg
//
LOCUS       DASUGJ010000850         3502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1526983, whole genome shotgun sequence.
ACCESSION   DASUGJ010000850 DASUGJ010000000
VERSION     DASUGJ010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526983"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFV93_19045"
     CDS             complement(<1..186)
                     /locus_tag="VFV93_19045"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5167515.1"
                     /translation="MTEDVRSGWRRSAARILPVFDWAPGYDRASLRADLIAGLTVAAL
                     VVPKSLGYAGIANVPIEH"
     gene            complement(470..2923)
                     /locus_tag="VFV93_19050"
     CDS             complement(470..2923)
                     /locus_tag="VFV93_19050"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5167516.1"
                     /translation="MAQDRFPDFTDALRRARRAAGISQEELAERSGLSARAISDLERG
                     VNRAPRRDTLEMLAEALDLSPDERKTWERLRHTVVARAKQERVATYAERSSSATAHSH
                     RESRRSNLPRPLTPLIGRERELSTIAEWLLDDTRRIVTLIGSGDTGKTRLAIEAGIQQ
                     HDAFHGSVYFADLAPITDPDLAMRTIAAATGIRSLGRDSPAVTLREVIRHQPVLLIID
                     NMEQVLDIASELAGVVTDCPNLRVLATSRAPLRVRGEHEFPVSPLKLPELGETSDLAI
                     MLQNDAVRLFTERAQAVYPGFAISNDNVAAVAQICVHLDGLPLAIELAAARVKLLSPQ
                     AMLPLMGHRLKLLAGGHRDLPARQQTLRATIDWSYELLKPEQQRLFRQLATFTGGWTL
                     EAADAISEASPATRTTESAQVLHVIDGHSALIDQSLVRRIERPEGGTRFAMFESLREY
                     GRDKLQDHHESDDLRERHAAYFLAMAEHAARALDADDPVPVLNQLASEHDNLRAALEW
                     FHLSRDAQRGLCLVGYLREFWYMRGHIAEGRAHTAEFLSLPNAAEPSPCRAMALTTAA
                     WLALRQNDAEAVQHATEALAIRRAAGDDFHVSFLLTIMGVAVDNLHRDLEQSRRYHEQ
                     SLAIARRTANVKGASMALACLGALACRQGDHNRAQILLDESIAIATAVGVTQPLALAL
                     GVAALMALDAGNPDRALALHQERLRLYVRLDDAWGILRTLHNLALVALARDQPTTATR
                     LMAVVASLREMTGLMASPWDQPAIESSRAELIARLGAAAFEAAWAAGSTLPKDDAIAE
                     ALAADANESRASYGRTVPA"
     gene            3130..3483
                     /locus_tag="VFV93_19055"
     CDS             3130..3483
                     /locus_tag="VFV93_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5167517.1"
                     /translation="MHMRPVIQRIGIAAVLIGIVLLNAVGTDEINAQEIDGAFAPAVM
                     PGSVPQGVVSASAEPSGPAKSTLRRLDLAVMHLDYLFLDEVVAGRGGVMTPEVEMAIA
                     LVDYLLLPEVAVSAS"
BASE COUNT          631 a         1081 c         1134 g          656 t
ORIGIN      
        1 gtgctcgatc ggcacgttcg caattcccgc gtatcccaga ctcttgggga cgacgagcgc
       61 agcgacggtc agtcccgcga tcaggtcggc gcgcagcgag gcgcgatcgt accccggcgc
      121 ccagtcgaaa acgggcagga tacgggctgc cgatcgtctc caaccggacc gaacgtcctc
      181 agtcatcgcg atgccctttc gaccgagtgt cccctgccga cgcacgacgg ttcggcgcgc
      241 aggtgaatcg gccgccgggt cggggccagt gtcgtgttgc taccgtcgct atcggagggg
      301 tcggccggct ggccgcgaat catctagcgg gtcgtagacc gggggtgtac gacccgccgc
      361 ccgaagaact cgcggcactt catcgcgagt cggcgctggt gcgtcctggt gagccatgtt
      421 cagcgtgacc acgacctgtc gccgtcggca tcatcgagag ggtgcaggat caggctggaa
      481 cggtgcgtcc atagcttgca cgcgactcgt tcgcatcagc tgcgagtgcc tcagcgatcg
      541 cgtcatcctt cggcagcgtc gagcctgctg cccaggcagc ttcgaacgcc gcagccccaa
      601 gcctggcgat taattcggcg cggctggatt caatggcggg ctgatcccac ggtgaggcca
      661 tgagtccggt catctccctc agcgacgcga cgacggccat caatcgggtc gcggttgttg
      721 gctggtctct ggcgagggca accagcgcga gattgtgcag cgttctcagg atgccccacg
      781 catcgtccag ccggacatag agtcgtaacc gctcctgatg cagcgccagt gctcgatcag
      841 ggtttccggc atccagcgcc atgagtgcgg cgacacccaa cgcaagcgcg aggggctgcg
      901 tgacgccgac tgcagtggcg attgcaatgc tctcatcgag caggatctgc gcccgattgt
      961 ggtcaccctg acggcacgcc aaggcgccga ggcatgccag tgccatcgac gcgcccttca
     1021 cgttagccgt tctgcgggca atagcgaggc tctgctcgtg gtagcggcga gactgctcaa
     1081 ggtctcggtg aaggttgtca accgctacgc ccatgatcgt gagcagaaac gatacatgaa
     1141 aatcgtctcc ggccgctcgc ctgatcgcga gtgcttccgt cgcgtgttgc acggcttcgg
     1201 cgtcgttctg gcggagtgca agccacgccg ctgtcgtcag cgccatcgcc cggcaaggcg
     1261 acggctctgc tgcgttcggc aagctgagga attcggctgt atgtgctcgg ccctcggcga
     1321 tgtggccgcg catgtaccag aactcgcgca ggtagccgac cagacacaga ccacgctgtg
     1381 cgtcacgcga cagatggaac cactccagcg cagctcgcag attgtcgtgc tcggatgcga
     1441 gttgattcag aaccggaacc ggatcatccg cgtcgagcgc ccgagccgca tgttcggcca
     1501 tcgccaggaa gtaggctgca tggcgttcgc gcaggtcgtc ggattcatga tggtcctgga
     1561 gcttgtcacg accgtactct cgaagcgact cgaacatggc gaatcgcgtt cctccttcag
     1621 gtcgctctat ccgtcgcacg aggctctggt cgatcagcgc gctgtgcccg tcgatgacgt
     1681 gtagaacctg ggccgattct gtggtgcgtg tcgctgggct ggcctcggag atggcgtcgg
     1741 ctgcttccag cgtccacccg ccggtaaacg tcgccaactg gcggaacagc cgttgttgct
     1801 ccggcttgag cagttcgtaa ctccagtcga ttgtggcgcg cagcgtctgt tgacgcgctg
     1861 gaagatcgcg atggccgccg gccagaagct tcagccggtg acccatgagc gggagcatcg
     1921 cttgtggaga caggagcttg acccgcgccg ccgccagctc gatcgccaac ggcaggccgt
     1981 caagatggac acagatctgg gcaaccgcag cgacattgtc gttcgagatc gcgaaccccg
     2041 gataaacggc ctgcgcacgc tcggtgaaca accgaacggc gtcgttttgc agcatgatgg
     2101 ccaggtcgga tgtctcgccg agttcaggca gtttcagcgg ggagaccggg aattcgtgct
     2161 caccacgtac cctcagcgga gcgcgggacg ttgcaagcac gcgcagattc gggcaatcag
     2221 taacgacgcc ggcgagctcg ctcgcgatgt cgagtacctg ctccatattg tcgatgatga
     2281 gaagcactgg ttgatgccgt atcacctcgc gtagcgtcac tgcgggggaa tctcgtccaa
     2341 gcgaccggat gcctgttgcc gcggcaattg tccgcatggc cagatcaggg tcggtgatcg
     2401 gagccagatc ggcgaagtag acgcttccgt gaaacgcgtc atgttgctgg atgcccgctt
     2461 ctatcgccag gcgggtcttg ccggtgtcac ccgatccgat gagagtgacg atccggcgag
     2521 tgtcgtccag aagccactcc gcaatggtgg agagttctcg ttcgcgaccg atcaacggcg
     2581 tcagcggccg tggcagattt gagcgccggg actcccgatg cgaatgtgcg gttgcggagg
     2641 acgaacgctc agcgtaagtc gcaacgcgct cctgcttggc acgggccaca acggtgtgcc
     2701 gcaaccgctc ccacgttttg cgctcgtctg gtgacagatc gagtgcctct gccagcatct
     2761 ccagcgtgtc acggcgtggg gcgcggttga caccccgctc cagatcgcta atggcacggg
     2821 cactcagacc ggaccgctcg gccagttcct cctgggagat tccggcagct cggcgagcgc
     2881 ggcgcagggc gtcagtgaag tcggggaacc gatcctgagc catttggacc accgatccgt
     2941 ggtgctgacc tgccaccagc atagcatcac gagtggttga caaagcccac atctcgactg
     3001 cacatgtgag tcaccctgtg gatttttcgt ggcgtgttgg cggagatcag cgcaatctgg
     3061 ggttggatcg aatgcttacc aggcagcacc ggaaaagaca accgatggga cacgacgatg
     3121 cagccccaga tgcatatgcg acctgtgatt cagcgtatcg gcatagcagc agtcctgatc
     3181 ggtatcgtcc tgctcaacgc cgtcggcacg gacgagatca acgctcagga gatcgacggc
     3241 gcgttcgcac cagcagtaat gcctggcagc gtccctcagg gtgtagtgag cgccagcgcc
     3301 gagccgagcg gtccagcgaa gtcgacgcta agacgcctgg acctggcagt catgcatctc
     3361 gactacctct tcctcgatga ggtagtcgcc ggacgtggtg gtgtcatgac tccggaggtt
     3421 gagatggcga tcgccctggt tgactacctg ctcttaccgg aagttgcggt ctccgcgtcg
     3481 tgaccgcaac gtatgctggc gg
//
LOCUS       DASUGJ010000851         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1527215, whole genome shotgun sequence.
ACCESSION   DASUGJ010000851 DASUGJ010000000
VERSION     DASUGJ010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527215"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFV93_19060"
     CDS             <1..261
                     /locus_tag="VFV93_19060"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5167518.1"
                     /translation="RAALLQVGQSLRPGGTIYVVGLILDDSRLTPPASARINVICLSF
                     YDGGQAYTEREYRAWLTEAGFTDITRDASVAGSSVLRARKAG"
     gene            complement(336..884)
                     /locus_tag="VFV93_19065"
     CDS             complement(336..884)
                     /locus_tag="VFV93_19065"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX5167519.1"
                     /translation="MTATTTSQSAALKTLAGAIRAIVQSESDPALVGEAVAVALKPNL
                     GDASLLTDDQLVPDADHYRQHILHVEPDGSFSIVALVWLPGQETPIHDHVCWCVVGVH
                     LGEEHETTYTLAGTDDDPHLVVSGQPVNLNGTVAALVPPGDIHKVANDGDSLAVSLHI
                     YGADIGTLGTSIRRRYDLPVRA"
     gene            1087..>2636
                     /locus_tag="VFV93_19070"
     CDS             1087..>2636
                     /locus_tag="VFV93_19070"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEX5167520.1"
                     /translation="MSLAWRSKHPGAVERETSATRTFSRRRLVVLAGVLVVLVLLCAG
                     YLLAVEQRYRAARESAHLIQDRYGPAGSEAGVDLKDLPTLKEDLARLEEDLGDLQGLV
                     DAPLVGRFARHAPVVGGNVATSQRFIAMGRELTAIARDATAIADETRLTFETSGMATS
                     DGASGPTWLDVVDAHRAEIEELERRFDAVLTERDAIDERQLPGSAQAMLPEIDRMLAK
                     AEGIRDEYYSLLPLFDLAFGANDKRNYVILLQNREEIRPGGGFPGTFAAVTLRDGQLA
                     EYAIYDIRTLDGDYVAKRQAPITAPGPIRKVLGQEEFLPHDAFWSPDFTESAATFESM
                     YAKAGWPELTGVVAVDDSVVREILAILGPYEVVFDGESQTVTADNFMELIEERRDLTW
                     QDLAAHKQVVAVLGSSLIDQIKAADFGTKKQIYFALREAADRRQVQVHMVEEALQAEV
                     VARGWDGALLPEPSVPTLATTVAGLTGGKKALTIFPEATIEIAPSGQGYRVRWTITLD
                     HRGDPNGNP"
BASE COUNT          448 a          795 c          914 g          479 t
ORIGIN      
        1 cgagctgcgt tgctccaagt cggccagtcg ctgcgtcctg gcgggacgat ctatgtcgtc
       61 ggactgatcc ttgatgattc gcgccttacc ccaccggcct ctgcccggat caacgtcatc
      121 tgcctcagct tctatgacgg cgggcaggcg tacaccgagc gcgagtatcg tgcctggctc
      181 acggaagcgg gctttaccga cattacgcga gacgcgtcgg tggcaggctc cagcgtgctg
      241 cgtgcgcgca aggcaggata accgtcccag atgcgcctcg ttctcgttgg tcgccgctct
      301 gtcacggatg tgcggcgtgg gaatgttgcc gagaactacg cacggaccgg caggtcgtag
      361 cggcggcgga tgctggtgcc gagcgtgccg atgtcggcgc cgtagatgtg gagtgagacg
      421 gccagtgagt cgccgtcgtt ggcgacctta tggatgtcgc ccggtgggac gagcgcggcg
      481 acagtgccgt tgaggttgac cggctgaccg ctgacgacca ggtgggggtc gtcgtccgtg
      541 ccggccagtg tgtaggtcgt ctcgtgctcc tcgccgaggt gcacgccgac gacgcaccag
      601 cagacgtggt cgtggatcgg tgtttcttgc cccggcagcc agaccagcgc aacgatcgag
      661 aagctgccgt ctggctcgac atgcaggatg tgctgacggt agtgatcggc atctgggacg
      721 agctgatcgt cagtgaggag cgatgcgtcg ccgagattgg gcttgagcgc gacagcgacc
      781 gcctctccaa ccagcgcagg atcggattcg gactggacga tcgcgcggat cgcgccggcc
      841 agcgttttga gggcagcgct ctgcgaggtg gttgtggcgg tcatcgttgc tcctgacagg
      901 tgtggcgaca agtcgatgcg gcaatcgtac tccaactgct atcgcagcat gcctgaatga
      961 atcgtcatga cactgacagg atttgtaacg gcgccgcgaa tcagctcaac gacgatgatt
     1021 gctcctgaca tcgtcccgcc tgtcgccact gtggctgcgg gctgatatgg gagcgtgagt
     1081 gccctgatga gcctggcctg gcgatcgaag catcccggcg ctgtcgagcg tgaaacgtct
     1141 gccactcgaa cattctcacg acgacggctg gtagtgctgg ctggtgtcct tgtcgtcctt
     1201 gtcctgcttt gcgcgggcta tctgctggcg gtcgagcagc gctaccgggc ggcgcgcgag
     1261 agtgcacacc tgattcagga tcgctacgga ccggccgggt ccgaggcggg tgtcgatctc
     1321 aaggatctcc cgacactcaa agaagatctg gcgcgtcttg aagaagacct cggcgacctc
     1381 cagggcttgg tcgacgcccc gctcgtcggc cgcttcgccc gccatgcgcc ggtcgtcggc
     1441 ggaaacgtcg cgacatcgca gcggttcatc gcgatggggc gcgagttgac cgcgatcgcc
     1501 cgcgacgcaa cggcgatcgc cgacgagacg cgcctgacat ttgaaacgag cggaatggcg
     1561 acgtcggacg gcgcatcggg cccgacctgg ctcgatgtcg ttgatgcgca tcgggcggag
     1621 atcgaggaac tggaacgccg gttcgacgcc gtcctgaccg agcgtgatgc gatcgacgag
     1681 cggcaactgc cgggcagcgc acaggcgatg ctgccggaga tcgaccggat gctggcgaag
     1741 gccgagggca tccgcgacga gtactacagc ctgcttcccc ttttcgacct ggccttcggc
     1801 gcgaatgaca aacgcaatta tgtcattctg ctccagaacc gcgaggagat tcgaccgggc
     1861 ggaggctttc cggggacctt tgcggccgtg acgctgcgcg acggccagtt ggccgagtac
     1921 gcgatctacg atatccgcac gctcgatggt gactacgttg ccaagcgaca ggcgccgatc
     1981 accgcgccgg ggccgattcg caaggtgctg gggcaggagg agttcctgcc gcacgatgcg
     2041 ttctggtcgc cggatttcac cgagtctgcc gcaacgttcg agtcaatgta cgcgaaggcc
     2101 ggctggccgg agctgacggg cgtcgtcgcc gtcgacgaca gcgtcgtccg tgagatcctc
     2161 gcgatccttg gcccgtacga ggtggtgttt gacggcgaga gccagacggt cacggcggac
     2221 aatttcatgg agctgatcga agagcggcgt gacctgacct ggcaggatct ggcggcgcac
     2281 aagcaggtcg ttgcggtgct cggctcatcg ctgatcgatc agatcaaggc ggccgatttc
     2341 gggacgaaga agcagatcta cttcgcactg cgcgaggcgg ccgatcgtcg ccaggtccag
     2401 gttcacatgg tcgaagaggc gctgcaggcc gaggtcgtcg cgcgtggctg ggatggcgcg
     2461 ctcctgccgg aaccatcggt gccgacgctg gcgacgacgg tcgccggcct gaccggcggc
     2521 aagaaagcgc tgacgatctt cccggaggcg acgatcgaga ttgcgccgtc gggccagggc
     2581 tatcgcgtcc gttggacgat cacgctcgat caccgtggcg accccaacgg caaccc
//
LOCUS       DASUGJ010000852         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1402
            k141_1250159, whole genome shotgun sequence.
ACCESSION   DASUGJ010000852 DASUGJ010000000
VERSION     DASUGJ010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961554
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,816
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,775
            CDSs (with protein)               :: 3,775
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250159"
                     /isolate="SMAG_U1402"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="VFV93_19075"
     CDS             <1..2083
                     /locus_tag="VFV93_19075"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX5167521.1"
                     /translation="YPRSVAVVGASPKGGYGLSVVTALTSFGFDGPIYPVNPNYDTIA
                     DLPAYPEIGAIPEPVDAVAIAVPSRAVPGVVQQAIAAGVKGGVAFGSGFAEAGEDGQS
                     LQVELRDLCGERFPLIGPNCLGVMSYLGGAALWSIPIKDRRRDGTVGLVAQSGNMALT
                     LMTNSRGLRLAHAVSAGNQAVVDAVDVMSFFLAEPGIRVIAAVIEGLSDVDKFRRVAA
                     RAAERDVPIVALKLGRSEKASRAAVAHTGSLTGSDQLYDALFRQYGVLRVDDLDELME
                     TAKLLSSDRRPAGSGLGVFASSGGECGLISDLAAASGVALPDLQPETREQLLGLLPPF
                     ANPLNPLDITASGWGNREIYGNVATLLSATPGVDIVAAIGDTTRNSGPLSATGWDRMI
                     AGLADARDRTDTPIALINTMIDAAYEMTDALAEQGIIHFSGARNAARAIGHAGHYARW
                     RREQRPKVDVSIDAARRTAALALLPKAGSGGLSETTSKQLLRHYGIPVPAGGLAENLD
                     EALALANEAGYPVVLKIEADDIHHKTEVGGVALGITSDEQLKEDFAALLERVAANAPG
                     ARVRGVRVERMASGLVELIVGGRNDPLFGPIVVAGLGGVMAEALQDAATRLAPIDATE
                     AASMLASLRGAALLGPFRGRPAVDVGAVAGIIARVSQLLAELPEVLELDLNPVLVGVE
                     GCVAVDGLVVV"
     gene            complement(2377..3597)
                     /locus_tag="VFV93_19080"
     CDS             complement(2377..3597)
                     /locus_tag="VFV93_19080"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5167522.1"
                     /translation="MDLRYTAEDEAFRTQVRAWLAEHAPREPLRTTVDKKAWHRKLYE
                     AGYLGMGWPKAYGGREARPVEQAIVIEEMARANAPGSINWAGLGLVGPTLIHHGSDAQ
                     RQRYLRNILLGDEVWCQLYSEPNAGSDLASLQCRADIDGDEFVVNGQKIWTSTAPEAD
                     MGILLVRTDRDAPKHQGISYLIVDMHQPGIEVRPLRQVTGIADEFAEVFFTEVRVPAD
                     NLIGELNAGWHIAQTTLSYERGGDTMGIVARLQQSFERLLEISDTPRHDGSRQIDDPL
                     VRQKLGQIKAEIEVLRYASLRILSAAEKGNPLGPESAISKLHYSEMDKRVSTLMLEIL
                     GPYGLLTNDAPAEYAFAPDGHGDAHDTWPYQYMHPFAETIYAGSSQIQKNIIGERVLG
                     LPKEVRADRLARTT"
BASE COUNT          560 a         1300 c         1318 g          676 t
ORIGIN      
        1 ctatccccgc tcggttgctg tcgtcggggc gtcaccgaag ggcggctacg ggctgagcgt
       61 cgtcactgcg ctcacgtcct ttggctttga tggtccgatt tacccggtca acccgaacta
      121 cgacacgatc gccgatctgc cggcctaccc ggagattggc gcgattccgg agccggtcga
      181 cgccgtcgcg atcgcggtcc cgtcgcgggc cgtgccgggc gttgtccagc aggcgatcgc
      241 cgccggtgtg aagggcgggg tcgccttcgg cagcggcttc gccgaagccg gcgaggacgg
      301 ccagtcgttg caggtggagt tgcgcgacct ctgcggcgag cgcttcccgc tgatcgggcc
      361 gaactgcctc ggggtgatga gctacctcgg cggcgcggcg ctctggtcga tcccgatcaa
      421 ggatcgtcgc cgggacggca cggtcgggct ggtcgctcag agcggcaaca tggcgctgac
      481 gctgatgacc aacagccgtg gactccggct ggcgcatgcc gtctcggccg gtaaccaggc
      541 cgtcgtcgac gccgtcgatg tgatgagctt cttcctggcc gagccgggca tccgcgtcat
      601 cgccgctgtc atcgaggggc tctccgacgt cgacaagttt cgccgcgtcg ctgcccgggc
      661 ggccgagcgg gatgtcccga tcgtcgctct caagctggga cgctcggaga aggccagccg
      721 cgcggccgtt gcccacaccg gctcgctgac cggttcggat cagctctacg acgcgctctt
      781 ccggcagtac ggcgtcctgc gcgtcgatga cctcgacgaa ctgatggaga cggccaagct
      841 gctctcctcc gaccggcgtc cggctggttc cggcctgggt gtcttcgcca gctccggcgg
      901 cgagtgcggg ctgatctccg acctggcggc ggccagcggc gtcgcgctgc ccgacctgca
      961 accggaaacg cgcgagcaac tgctcgggct gcttccgccg ttcgcgaacc cgctcaaccc
     1021 gctcgacatc accgcctccg gctggggtaa ccgcgagatc tacggcaacg tcgcgacctt
     1081 gctgtcggcg acaccgggcg tcgacatcgt cgccgcgatt ggcgacacaa cccgcaactc
     1141 cggcccgctc tccgcgaccg gctgggacag gatgatcgcc gggctggccg acgcccgcga
     1201 ccggaccgat accccgatcg ccctcatcaa cacgatgatc gacgccgcct acgagatgac
     1261 cgatgcgctg gccgagcagg gcatcatcca cttctccggc gcccgcaacg ccgcccgcgc
     1321 gatcggccac gccggccact acgcccgctg gcggcgggaa cagcggccga aggttgatgt
     1381 gagcatcgac gccgcgcgcc ggactgccgc gctcgccctc ttgccgaagg ctggcagcgg
     1441 cggcctctcc gagacgacca gcaagcaatt gctgcggcac tacggtatcc cggtcccggc
     1501 cggcgggttg gctgaaaacc ttgacgaggc gctggcgcta gccaacgagg ccggttatcc
     1561 agtcgtcctg aagatcgagg ccgacgacat ccaccacaag accgaggttg gcggcgtcgc
     1621 gctcggcatc acgtcagacg aacaactcaa ggaggacttt gccgcgctgc tggagcgcgt
     1681 cgctgccaac gcgccgggcg cacgggtacg cggcgttcgc gtcgagcgga tggcgtccgg
     1741 cctggtcgag ctgatcgtcg gcgggcgcaa tgacccactc ttcggcccga tcgtcgtcgc
     1801 cggtctcggc ggcgtcatgg ccgaggcgct ccaggacgcc gcaacccgcc ttgcgccgat
     1861 cgacgctacc gaagccgcct ccatgctcgc cagcctgcgc ggcgcggccc tgctcggccc
     1921 cttccgcggc cgcccggcag tcgatgtcgg cgccgttgcc ggcatcatcg cccgtgtctc
     1981 gcaactgctg gctgagctac ccgaggtgct ggaactggac ctcaacccgg tgctggttgg
     2041 ggtggaagga tgtgttgcgg tggatgggtt ggtggtggtg tgaagacgtg acgcgtgacg
     2101 cgtgatgcgt gacgcgaacc agacgaatgg cgctggcgag gaccgcacca ctagccctgt
     2161 catcctgagc cgcagcgaag gatcgcctaa cgtacgctac aggagcgcgt atgctatagg
     2221 agcggttcgg gagagccacg aacgttctgc cggtcggcgc gctcgtgtcc ccaacgccaa
     2281 gcgatccttc gctgcggctc aggatgacag ggcaaggggc cgtgttcctc cggtgagccg
     2341 ttcgtctggt tcgcgtcacg catcacgcgt cacgcgtcac gtcgtccgcg caagccgatc
     2401 cgcccgcact tccttcggca acccgagcac acgttcgccg atgatgttct tctggatttg
     2461 cgaggaaccc gcgtagatcg tctcggcgaa ggggtgcatg tactggtagg gccaggtgtc
     2521 gtgtgcgtcg ccgtggccgt cgggtgcgaa ggcgtactcg gccggtgcgt cgttggtgag
     2581 caggccgtag gggccgagga tctccagcat cagcgtcgag acgcgcttgt ccatctccga
     2641 gtagtgcagc ttggagatgg ccgactccgg cccgagcggg ttgcccttct cggcagccga
     2701 gaggatgcgc agcgacgcgt agcgcagcac ctcgatctcg gccttgattt gccccagctt
     2761 ctgacgcacg agcggatcgt cgatctggcg gctgccgtcg tggcgcggtg tgtcgctgat
     2821 ctccagcagt cgctcgaacg actgctgcag gcgggcaacg attcccatcg tgtcgccgcc
     2881 ccgctcgtag gagagcgtcg tctgggcgat gtgccaaccg gcgttcagct cgccgatcag
     2941 gttgtcggcc gggacgcgca cctccgtgaa gaacacctcg gcgaactcgt cggcgatgcc
     3001 ggtcacctgc cgcagcgggc gcacttcgat gccgggctgg tgcatatcga cgatcagata
     3061 ggagatgccc tggtgcttcg gcgcgtcgcg gtcggtgcgg accagcagga tgcccatgtc
     3121 cgcttccggc gcggtgctgg tccagatctt ctggccgttg acgacgaact cgtcgccgtc
     3181 gatgtcggcg cggcattgta gcgacgccag gtcggagccg gcgttcggct ccgagtagag
     3241 ctggcaccag acctcgtcgc cgagcaggat gttgcgcaga tagcgctggc gctgggcgtc
     3301 gctgccgtgg tggatcagcg tcggcccgac tagccccagc ccggcccagt tgatcgagcc
     3361 gggcgcgttg gcccgcgcca tctcctcgat gacgatcgcc tgctcgaccg gccgcgcctc
     3421 gcgcccgccg tacgccttcg gccagcccat gccgaggtag ccggcttcgt agagcttgcg
     3481 gtgccatgcc ttcttgtcga cggtcgtccg caacggctcg cgcggggcat gttccgccag
     3541 ccaggccctc acctgcgtcc ggaaggcttc atcctcggct gtgtaccgta gatccatcgt
     3601 tgtcgctctc catgttgccc tgctggttgg ggggtcggga cgaattggat gacgtggttg
     3661 gcagtatacc ggggtcgtcg cgggggtgcc gtagtccgtc acggatcgta gtgatagcgt
     3721 gcctggagaa aggtgatccg atcgtccgtc acgtgataga ccagccggtg ttcctgggta
     3781 atccggcgcg accacacgtc aggatcaaga tacttcaggc gttccggctt gccgatccct
     3841 gcgaacggat cgcg
//
